LOCUS       DAEMAE010000001        62024 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEMAE010000001 DAEMAE010000000
VERSION     DAEMAE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.001"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..373
                     /locus_tag="KPH93_000001"
     CDS             230..373
                     /locus_tag="KPH93_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686849.1"
                     /translation="MDDDINMIAIRPEDIKVEFNRDFKEVDIKVREILDIEFRRFNYI
                     VTV"
     gene            711..1778
                     /locus_tag="KPH93_000002"
     CDS             711..1778
                     /locus_tag="KPH93_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1686850.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTI
                     YTAIEEDSIDTYLTTFKEKYPDIKLNILRLSVGNITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELKAKNIST
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1970..2500
                     /gene="rnmV"
                     /locus_tag="KPH93_000003"
     CDS             1970..2500
                     /gene="rnmV"
                     /locus_tag="KPH93_000003"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG1686851.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPKSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVERDEFIDALKQI"
     gene            2671..3540
                     /gene="rsmA"
                     /locus_tag="KPH93_000004"
     CDS             2671..3540
                     /gene="rsmA"
                     /locus_tag="KPH93_000004"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG1686852.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCETEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EEIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3606..4865
                     /locus_tag="KPH93_000005"
     CDS             3606..4865
                     /locus_tag="KPH93_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686853.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKEDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEELEEEDEEIEDNEESDRDEDSEEEEY
                     IIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKVQE
                     EYIDIEYDEEEEDEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQGE
                     SGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLGY
                     LNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFLR
                     YQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            5016..6131
                     /locus_tag="KPH93_000006"
     CDS             5016..6131
                     /locus_tag="KPH93_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG1686854.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVLI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6317..7213
                     /locus_tag="KPH93_000007"
     CDS             6317..7213
                     /locus_tag="KPH93_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1686855.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7283..8080
                     /locus_tag="KPH93_000008"
     CDS             7283..8080
                     /locus_tag="KPH93_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686856.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(8135..9487)
                     /locus_tag="KPH93_000009"
     CDS             complement(8135..9487)
                     /locus_tag="KPH93_000009"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG1686857.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9706..10584
                     /gene="purR"
                     /locus_tag="KPH93_000010"
     CDS             9706..10584
                     /gene="purR"
                     /locus_tag="KPH93_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG1686858.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10740..11018
                     /gene="spoVG"
                     /locus_tag="KPH93_000011"
     CDS             10740..11018
                     /gene="spoVG"
                     /locus_tag="KPH93_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG1686859.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11231..12610
                     /gene="glmU"
                     /locus_tag="KPH93_000012"
     CDS             11231..12610
                     /gene="glmU"
                     /locus_tag="KPH93_000012"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG1686860.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12705..13655
                     /locus_tag="KPH93_000013"
     CDS             12705..13655
                     /locus_tag="KPH93_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG1686861.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14496..14581
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14743..15264
                     /locus_tag="KPH93_000014"
     CDS             14743..15264
                     /locus_tag="KPH93_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1686862.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLNVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGKDGEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDQLKDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            15416..17092
                     /locus_tag="KPH93_000015"
     CDS             15416..17092
                     /locus_tag="KPH93_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG1686863.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIGVNESITIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            17104..18312
                     /locus_tag="KPH93_000016"
     CDS             17104..18312
                     /locus_tag="KPH93_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG1686864.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTPVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILVSAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18327..20039
                     /locus_tag="KPH93_000017"
     CDS             18327..20039
                     /locus_tag="KPH93_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686865.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSEIPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLILDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQLKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYNMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTADK
                     SSNNEIKSNTKSDSNGDKTNNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFEDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            20032..20940
                     /gene="pilO"
                     /locus_tag="KPH93_000018"
     CDS             20032..20940
                     /gene="pilO"
                     /locus_tag="KPH93_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG1686866.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVREYEHFYGVSTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTSYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20967..21533
                     /locus_tag="KPH93_000019"
     CDS             20967..21533
                     /locus_tag="KPH93_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1686867.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKRQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKH
                     TEVYSVTRIQKNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNINLNNKTYTFDVMSRIG"
     gene            21570..22097
                     /locus_tag="KPH93_000020"
     CDS             21570..22097
                     /locus_tag="KPH93_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1686868.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     SDVSLYDIPYEKITTIKDEDGNLIDKENITNKIKTIVEDKSGQIYKVAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            22118..23656
                     /locus_tag="KPH93_000021"
     CDS             22118..23656
                     /locus_tag="KPH93_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686869.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSELEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEHILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPNSQEYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEVDKTEGISIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDSKKNIIGLGVEENSNSKYKVEAIVNFNLNIDTNVVGNYNIKDKILINHDISSY
                     KKVN"
     gene            23756..24838
                     /locus_tag="KPH93_000022"
     CDS             23756..24838
                     /locus_tag="KPH93_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG1686870.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPENIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKSGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24819..25601
                     /locus_tag="KPH93_000023"
     CDS             24819..25601
                     /locus_tag="KPH93_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG1686871.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSIIFNLLFKEIPVKDSIIGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSIYVGAVYGICVII
                     TSRIKGKEYNSVIPYGPFISIGALITILCGKQLLEFYFSMVI"
     gene            25703..26353
                     /locus_tag="KPH93_000024"
     CDS             25703..26353
                     /locus_tag="KPH93_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG1686872.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYSLLYKFI"
     gene            26367..26927
                     /locus_tag="KPH93_000025"
     CDS             26367..26927
                     /locus_tag="KPH93_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG1686873.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKIDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEVGQDLADFVLSRFRKEESDNINE
                     ALEKAAYAIDSIIRENIDMSMNKYNG"
     gene            26944..30330
                     /gene="mfd"
                     /locus_tag="KPH93_000026"
     CDS             26944..30330
                     /gene="mfd"
                     /locus_tag="KPH93_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG1686874.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30361..31356
                     /locus_tag="KPH93_000027"
     CDS             30361..31356
                     /locus_tag="KPH93_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1686875.1"
                     /translation="MKKVITLVITMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDASASDGGKLGFFSRGQMVAEFEDAAFSMKKGEISDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKEQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31480..32019
                     /gene="spoVT"
                     /locus_tag="KPH93_000028"
     CDS             31480..32019
                     /gene="spoVT"
                     /locus_tag="KPH93_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG1686876.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            32139..33752
                     /locus_tag="KPH93_000029"
     CDS             32139..33752
                     /locus_tag="KPH93_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG1686877.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33859..35307
                     /gene="mazG"
                     /locus_tag="KPH93_000030"
     CDS             33859..35307
                     /gene="mazG"
                     /locus_tag="KPH93_000030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG1686878.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIEVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKIEDMTLEEMDEFWNQAKKQ"
     gene            35396..35674
                     /locus_tag="KPH93_000031"
     CDS             35396..35674
                     /locus_tag="KPH93_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG1686879.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35746..35988
                     /locus_tag="KPH93_000032"
     CDS             35746..35988
                     /locus_tag="KPH93_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG1686880.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            36050..36304
                     /locus_tag="KPH93_000033"
     CDS             36050..36304
                     /locus_tag="KPH93_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG1686881.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36305..36838
                     /locus_tag="KPH93_000034"
     CDS             36305..36838
                     /locus_tag="KPH93_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686882.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36844..37128
                     /locus_tag="KPH93_000035"
     CDS             36844..37128
                     /locus_tag="KPH93_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG1686883.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINNLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37241..38161
                     /locus_tag="KPH93_000036"
     CDS             37241..38161
                     /locus_tag="KPH93_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG1686884.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKLVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCSDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEVYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38251..40623
                     /gene="spoIIE"
                     /locus_tag="KPH93_000037"
     CDS             38251..40623
                     /gene="spoIIE"
                     /locus_tag="KPH93_000037"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG1686885.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAIYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40974..42866
                     /gene="pepF"
                     /locus_tag="KPH93_000038"
     CDS             40974..42866
                     /gene="pepF"
                     /locus_tag="KPH93_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG1686886.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDAWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42980..43945
                     /gene="galU"
                     /locus_tag="KPH93_000039"
     CDS             42980..43945
                     /gene="galU"
                     /locus_tag="KPH93_000039"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG1686887.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEDMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEANGARELEVQK"
     gene            44445..46049
                     /gene="rho"
                     /locus_tag="KPH93_000040"
     CDS             44445..46049
                     /gene="rho"
                     /locus_tag="KPH93_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG1686888.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIRNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46202..46402
                     /gene="rpmE"
                     /locus_tag="KPH93_000041"
     CDS             46202..46402
                     /gene="rpmE"
                     /locus_tag="KPH93_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG1686889.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46547..47440
                     /locus_tag="KPH93_000042"
     CDS             46547..47440
                     /locus_tag="KPH93_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG1686890.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFIRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47458..48306
                     /gene="prmC"
                     /locus_tag="KPH93_000043"
     CDS             47458..48306
                     /gene="prmC"
                     /locus_tag="KPH93_000043"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG1686891.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFIGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDVSEIALEIAKKNSIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKRGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48350..49414
                     /gene="prfA"
                     /locus_tag="KPH93_000044"
     CDS             48350..49414
                     /gene="prfA"
                     /locus_tag="KPH93_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG1686892.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49543..50223
                     /locus_tag="KPH93_000045"
     CDS             49543..50223
                     /locus_tag="KPH93_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG1686893.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50252..51292
                     /locus_tag="KPH93_000046"
     CDS             50252..51292
                     /locus_tag="KPH93_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG1686894.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKKEVYDLAKNISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNIIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51309..51758
                     /locus_tag="KPH93_000047"
     CDS             51309..51758
                     /locus_tag="KPH93_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG1686895.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMSASENSIEALKEIGIDLSNHKSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRNTMREIMYSVNEIVKKI"
     gene            51775..52227
                     /gene="rpiB"
                     /locus_tag="KPH93_000048"
     CDS             51775..52227
                     /gene="rpiB"
                     /locus_tag="KPH93_000048"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG1686896.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLEGKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52263..52892
                     /gene="upp"
                     /locus_tag="KPH93_000049"
     CDS             52263..52892
                     /gene="upp"
                     /locus_tag="KPH93_000049"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG1686897.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIEEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            53191..54006
                     /locus_tag="KPH93_000050"
     CDS             53191..54006
                     /locus_tag="KPH93_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG1686898.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKEVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54232..54669
                     /locus_tag="KPH93_000051"
     CDS             54232..54669
                     /locus_tag="KPH93_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG1686899.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54854..55081
                     /locus_tag="KPH93_000052"
     CDS             54854..55081
                     /locus_tag="KPH93_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG1686900.1"
                     /translation="MSKKNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            55086..55472
                     /locus_tag="KPH93_000053"
     CDS             55086..55472
                     /locus_tag="KPH93_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG1686901.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55475..56179
                     /gene="atpB"
                     /locus_tag="KPH93_000054"
     CDS             55475..56179
                     /gene="atpB"
                     /locus_tag="KPH93_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG1686902.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGQILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56248..56508
                     /gene="atpE"
                     /locus_tag="KPH93_000055"
     CDS             56248..56508
                     /gene="atpE"
                     /locus_tag="KPH93_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG1686903.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56631..57128
                     /gene="atpF"
                     /locus_tag="KPH93_000056"
     CDS             56631..57128
                     /gene="atpF"
                     /locus_tag="KPH93_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG1686904.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            57125..57670
                     /locus_tag="KPH93_000057"
     CDS             57125..57670
                     /locus_tag="KPH93_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG1686905.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57687..59189
                     /locus_tag="KPH93_000058"
     CDS             57687..59189
                     /locus_tag="KPH93_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG1686906.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59217..60095
                     /gene="atpG"
                     /locus_tag="KPH93_000059"
     CDS             59217..60095
                     /gene="atpG"
                     /locus_tag="KPH93_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG1686907.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIVKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            60108..61502
                     /gene="atpD"
                     /locus_tag="KPH93_000060"
     CDS             60108..61502
                     /gene="atpD"
                     /locus_tag="KPH93_000060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG1686908.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61505..61765
                     /gene="atpC"
                     /locus_tag="KPH93_000061"
     CDS             61505..61765
                     /gene="atpC"
                     /locus_tag="KPH93_000061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG1686909.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25109 a         6614 c        11492 g        18809 t
ORIGIN      
        1 taaataatta taaactaaag taaataaatt attgagcgat tattaaaaag atatgtataa
       61 attttaattt gggaacttgt attatatatc atgaaaaaag agaaaaaata aaaagtaaat
      121 tgtttcttga taacttgaaa ttggaagtga gaatatttaa ctagagaaga attattataa
      181 gttcaggtta gctctaggag tagctgattt tataggaaca atagatttta tggatgatga
      241 tataaacatg atagcaatta gaccagaaga tataaaggta gaatttaata gagatttcaa
      301 agaagttgat ataaaagtac gagagatttt ggatattgaa tttagaagat tcaactatat
      361 agttacagtt taatatcgtt ctaagcaaat aaagttgaat gtagtttcaa atgtagtgga
      421 acaaatggaa ctatgtgtag gttataaaat aaacttcaag atacctaaag aaagaatcat
      481 ataatataag taatccatta atttttttat agacctcaaa ctcagaccgc atcgatataa
      541 aaactaaagc tagatgatat agtgttgtat ataggaccat cagctgttat ggcagtatta
      601 gtgcttttaa caaatataat agtaaaactt ttgtatgaat tgataataaa aaaattaaaa
      661 accaagacat aaaaatggca acaaaaagaa gttgaataag ggaggtaaaa atgaaattta
      721 agaagttagt ctctttggga atattatcaa cacttttatt gagtgtaata actggatgta
      781 gtaatgtttc atcaggttca aacttaaagt ctaataataa gacaaaagga gagttaacaa
      841 tttatacagc aattgaagaa gatagtatag atacatattt aactacattt aaagagaaat
      901 atccagatat taaattgaat atacttagat tatcagtagg aaatataaca gcaaggttat
      961 tagcagaaaa agataatcct aaagcagatg tgttatgggg ggttgcagct acaagtttga
     1021 taattttaga tgacaaagaa atgctagagc catacgctcc gaaaggcttt gataaggtaa
     1081 atcctgtatt taaagatgat aagaaagttc cttcttgggt tggaatagat gcttggatga
     1141 ctggtataac agtaaatact aaagaattaa aagctaaaaa tatatcaact cctagttctt
     1201 acgaagattt aataaagcca gaatataaag atcttatatc tatgcctagt ccggcttctt
     1261 caggaacagg atatttaaca gtatcggaac tcatacaaat tatgggtgaa gaaaaagcat
     1321 ggcagtacat ggataaatta cataaaaata ttggtgtata cactcagtct ggttcagctc
     1381 cagctatatt agcagcttca ggagaatatc ctattggtat atcttttgga aatagaggta
     1441 taaagttaaa aggtgaaggt tatccagtag aagtggtatt tccaaaagaa ggttctggat
     1501 gggagataga atcaaatgca cttgtgaaaa aagataatat aaaagaagca tcaaaagtat
     1561 ttttagattg ggctataagt aaagatgcaa tgaatgagta ttctaagaat tttgcagtta
     1621 ctacaattga cacgggaaat ccagtgccag aaggatttcc aaagaatccc ttagaacaga
     1681 tgatagataa tgatttaaaa tcagcagcaa gaaatagaga agctatacta aataaatgga
     1741 tagaaaaata tgatggaaag acagaaaaag agagctaagc tttacaataa aatttagtag
     1801 aaaaagcgcc tagaatataa attttatatc taggctcttt tgatatattt atttaaaatg
     1861 catatttcta tataaaaata aatagtttag taccttgatt aaattaaata aaaaccttaa
     1921 aataaagaga tgtaaagaaa aactaaaaac gaataaaagg tgaatttata tgataaaaga
     1981 aataatagta gtagagggta gagatgacgt cacagcagtt aagagagcaa tagatgcaga
     2041 acttataact acaggtggat ttggatttcc aaaaggtgta atggagcgta taaaagctgc
     2101 taatgaaaga cgtggtgtta taatatttac agacccagat tttgcaggtg aaaaaataag
     2161 aaaaaaaata gcatcagaag ttccaggatg taaacatgca tttctaccaa gagaggaagc
     2221 taaaaaggat ggagatatag gtattgagaa tgctactcct aaaagtataa tagcagcttt
     2281 gaataaagtt agaactgaaa atacagaaaa aagagatgaa tttaagcaag ttgacttaat
     2341 tagaaatggt cttataggta atgaagaagc atcacttaga agagatgcac ttggaaaaat
     2401 acttggaatt ggatatggaa atgcaaaaca atttttaaat agattgaata attatggagt
     2461 tgaaagagat gaatttatag atgcattaaa acaaatctaa acgtaatgtt taatgtatga
     2521 aaaatatgta tataaaataa attgtggaat tacggttaat ttaagataaa ttatttataa
     2581 tatatacctt attttaaatc aatgtaaata catataaata agtataatta ataaaattaa
     2641 atatcaaaat aagttaaaaa ggagtaaata atggatagac tatcttcaca taatgcaaca
     2701 aaggaagtag tacaaaaata taattttaaa ttttcaaagt ctttaggtca aaatttcttg
     2761 atagatagta atataattga taaaatttta tcgggagcta gaatcacaag aggagataat
     2821 atcatagaag taggacctgg tataggaact cttactcgtg agatgggtaa aatagcagaa
     2881 aaagtagtgg ctatagaaat cgatagaaat ttaattccta tattgaaaga taccttgtca
     2941 gatttagata atacagaggt tgtaaatcaa gatatactaa aagtagacat acaggaatta
     3001 gtaaaagata aattaaatgg tggaccagtt aagcttgtag caaatcttcc atattatata
     3061 actactccaa tagttatgaa atttttagag gaagatatac ctgtaacaga tatagtagtc
     3121 atggttcaaa aagaggttgc agatagaatg aatgctattc caggtactaa agattatggg
     3181 gcattatcta tagctgttca atactattgc gagacagaga ttgtggcaaa agcgccaaga
     3241 catatgttta taccacaacc aaatgtagat tccacagtta taggtctaca tgtaagagat
     3301 aaaagaaaat atgatgttca taatgaagaa atatttttta aaacagtaaa agcatccttt
     3361 ggacaaagaa gaaaaactct tcttaattcg ttgggaggtc ttggttttct taataaagat
     3421 gaaataagag aaatattaaa agaagctaat atagacgaaa aaagaagggg tgaaactctt
     3481 agtatagaag agttttcagt tctttcaaat ataataaata caaaagtatc ttctaaatag
     3541 aaggtacttt tttttatttt gttaacatat attgtttaga gaaactgtgt taagggggga
     3601 ttgcagtggg ttctaaaaga aagtttaaaa gagactatat aatactagaa gctaaagaca
     3661 taaactttag atacaaagaa cgagtactgc caaaagcttt tgcaaaagtt gaagtaaatg
     3721 atgaaaaagc aattattgca ttatatgtag aaaatctaaa atatgttaaa gatgggtata
     3781 aagcagtagc aataaaagaa gaccatggat taatagactt aggaaatata attttaaatg
     3841 aacaaggaag aggtgaattt gttttagatt tagaagatta tgatgatgag ataaaaggta
     3901 ttgcattatt gtatgaaaga aatatacctt taatcggatt taagggaaat aagcttgaaa
     3961 attatgagga catattattt caagaggtag aggaagaaga atatgaagag ttggaagaag
     4021 aagacgaaga gatagaggat aatgaggaaa gtgatagaga cgaagatagt gaagaagaag
     4081 aatatattat agaagaatat gatgaagatg agtatgaaga ctattgggaa gatgatgaag
     4141 atattgaaga aggagagtta aaggcaagaa cggaagtaga agattctggt gatgaggaaa
     4201 tagaagagtt caataattat aaggaaatag aaaattataa tagggttaaa gtacaggaag
     4261 aatatataga catagaatat gatgaggaag aagaggatga agaagaagtt tttgaagtaa
     4321 atagagaaga agataggttt gtttcctcaa aacctactaa atcaaagaaa gttcaaaaag
     4381 atcagaataa tattgaaaaa ataaaaagag ctaatggagt aagtcaagga gagagtggaa
     4441 ataaacaaac aagtacatta cttatgccaa gacaaataaa aaaaggtttg aaattattta
     4501 aagaagtaaa accttttgta acagattata tagataagac aagatggtgg aaaatagaga
     4561 taaatcctac aactttgtgt ggatatacaa tgccttattt aggatattta aacgtattga
     4621 attacaccat gtatagtgat gcagttttac attcatataa atacagacac tatttatttg
     4681 gagttcagta tgatgaatat aataagagaa aacattatat ttatgctatc ccaggaaata
     4741 gaaatgaaca gccagacaat ggaagcacag gatttttaag atatcaagct tgtgataata
     4801 gaacaaatag tttaggatat tggttatgtt ttatagattc tagacaaaga aatatagtta
     4861 gataaaaata ataaaaatga aaaaatttag actcaattct tattttactg aaaaaattaa
     4921 tgaaatgtag tacaaaactt taaaaactat tgataatatt taaatattac actacaatat
     4981 atcttgaaat acctattata ttatggagga aaattatgat aaacgagcaa agaattttaa
     5041 atgagtttct tgaattagta cagatagata gtttatcttt aaaagagggg aatgttgcta
     5101 aagtattagt taaaaaatta gaagaaatag gttgttcagt attaatagac aatgctggtg
     5161 aaaaagcaaa tggagaaact ggtaatataa tagctacatt aaagggaaat aaagaaggaa
     5221 agaaaatatt atttagttca catatggata cagtaactcc aggtataggg gtaaaaccta
     5281 tagtggatga agcaaatgga ataataaaaa gtgatggtac tacaatatta ggttctgatg
     5341 ataaagcagg tatagcagct atattagaag gcttaagata tataaatgaa aataacatag
     5401 aacatactga tatacaagtt gtattctcta tatgtgagga atgtggatta gttggagcta
     5461 aaaatttaga ctatggaaaa atagattctg aatatgcgtt tatattagat agtggtggtt
     5521 ctccaggtga aataatagta aaagctcctg cacaagatgt aataaatgtt aagatattag
     5581 gaaagactgc tcatgcaggt cttgaacctg aagctggaat aagtgctata atggtagctg
     5641 ccagagcaat agaaaatatg aatttactta gaatagacga agaaacaact gctaacatag
     5701 gaattataaa cggtggaaca gctacaaata tagtaacagg agaagttaat atagttgcag
     5761 aagccagaag tttaaaagaa aataaattag atgttcaaac taaacacatg gtagagactt
     5821 ttgaaaaagc tgcaaaagat tttggagctc aaattgagat agatgtcaat agagcatata
     5881 caccaataga tgttagtgaa gattctgaaa taataaaact tgcaaagaag gctttttcaa
     5941 acttaggtat agaaggtcat actgaatcta caggaggagg tagtgataca aatatattaa
     6001 gtaagaatgg tatcgaagct ataactttag gaatcggtat gaaaaatgct catacactaa
     6061 gtgaacatat agctataaaa gacttatacg attctgctaa aatggtagtg gagataataa
     6121 aggaagcata aatttaaata atagtgtata atatatatat cgcctagctt aagctaggtg
     6181 atttttcatt ttatagaaga ttgataatct ctattgaaag taaagagtat acatttgcat
     6241 ataagagaga gtttaattta tgttcagatg taggttctta ataatattta gattgtttca
     6301 taagggagga ataaatatgg aaacaaggta tgaagaagca aataaaataa caatacaatc
     6361 aatactttgg aatgtagtat taacaataat taaggttata gctggtgtta ttggtaattc
     6421 tagcgctatg attgcggatg gattacattc agcatcagat attataagtt ctataggagt
     6481 actaataggc aattatgtat cttctagacc tggagataga gagcataatt atggtcatga
     6541 gaaagcagag acattagtat catttgtgtt atcaatattg cttattttcg tttctataac
     6601 aataggtata gaggcaatta aatctttatt taacttagat gcattaagtg ttccatcaat
     6661 attaccatta gtagtatcag ttatttcaat cctgataaaa gagtatcaat atagaataac
     6721 aataaaagtt gctaaaaaga taaattcacc agccctaaaa gctgatgctt ggcatcatag
     6781 gtcagatgct ttatcttcag tagctgcttt tataggtata ggaggttcta ttttaggatt
     6841 taagccatta gacccaattg catcagttgt agtggctata tttgtagcta aagtaggaat
     6901 tagtatactt ataagttctg ttaatgaatt gatggatgtt tcagttgatg aagaagagat
     6961 aaaagaatta aaatttattg tagcagatac agaaggtgtt aaaaatcttg gtgatataaa
     7021 gactagaaaa catggagcta tggcatatgt agatttgact atatgtgtgg atgaaaattt
     7081 gacagttaag caaggtcatg atattgctac taagcttgaa aaacatataa taaagcatat
     7141 ggaatttgta aaaggcataa cagttcacgt tgaaccatgt actaattgcc aaggaaataa
     7201 gtgtaataat taattaaaac ttaaaaaaaa gtgtaaaaag ttatataatt gttttatgat
     7261 tataaactct aggggtggtt aaatgagcaa gaaaagaaaa ctaaagaaaa aagctgcaat
     7321 attaatagga gtagttgttt tctttataat atactttata tcctttagaa taggaataaa
     7381 tatggaaaag gataaaagtg aagtgccaaa gaaggtagaa aaagaagaag aagataatag
     7441 gtctttaagg actcaaataa aagaaaagaa gaaggtttat atatcagaca aaaatataga
     7501 aaacataaaa attgaagaag atatgtggga tgaaataaag ttatttttct cagattttaa
     7561 gaaagtaagg aatactactt cttcttacga agcagtttat gaaggttatt ctgatgatgg
     7621 agttaaattt tctactgacc ttaacttttt tagagtatat actgtaaata aagaagaata
     7681 ttataaagta ccagttagtt ctaaggatgc ttttaagaaa cttttagatg agagtatata
     7741 tacttcgttt gaccttttaa gtcattataa agattggaaa gaggttacta taactcatgg
     7801 aaaagaaatt aaaaaagttc acagatggaa atttgatgat ttagcacata agatgatttc
     7861 aaaaagggtt gtaggaaaag ttcaacctga gaaaagtaag gaaagaagtg actataattt
     7921 tactataaat ataaaaggtg ataattttga tgtaaaagtt gatacaatgg gaaaagatta
     7981 tgtaaaagtt acttctaaga aaaatgtatc ttactatgag gttcatacaa gtttatttga
     8041 ttaccttaaa aatgagatat ttgaaattgg aaagaaataa gactttaaga aggctatcaa
     8101 attagatatt tgatagccta aattatttta tatattattt ttcaaccaac aagtctgcaa
     8161 ccttataaat tggaccagca ccagcagtta ttactaaatc attgtcctta acattttcac
     8221 gtaagtattt tactatatct tcaaattctt ttatatagat agcatctaca ttgttttgat
     8281 ataatttctc aaccaagtcc ttagaatgta tatctcctgg gtctttttcc ctagcagcat
     8341 atatatccgt tatgataact ttatcagcag catagaatgc ctctgaaaat tcacctaaaa
     8401 gagattttgt tctagtataa gtatgtggct ggaagataca ccataaagtg gattttttta
     8461 attttttggc agcagctaaa gtagctttta actcagttgg atgatgtgca taatcatcta
     8521 ctactaaagc attcttataa taccctttag tttcaaatct tcttccgaca cctttataaa
     8581 tttttatatt ttttcttata gtctctaagt caataccaga aacaagagct gccattatag
     8641 cagaagaagc attgtatatg ttatgaagac catatacaga aagttcaaat tctcctaaat
     8701 ctcttccttc atattctatt ctaaatattc catgaccact attgtcaaag tgaatatctt
     8761 ttattacagc atcattgtca gaatctctac catattttat aatagtagct ttaacatcat
     8821 acaatatgtc atcaatattt tcatcatcgc catttattat gaaatagcct tctgggggta
     8881 ataattttcc aaatttgtta aaagaagcct tgatttcatc tattccagag aaataatcaa
     8941 gatggtcttc ttcaacattt aaaactatag atatttttgg gttaaaatta aggaaactat
     9001 ctacatattc gcatgcttca gttataaagt ggttggaatt acctatctta acattaccac
     9061 ctatcatact taagttacca ccaactagta tagttgggtc taagtctgca tattcaaaga
     9121 ttgtagataa catagatgtt gtagaagttt ttccatgtgt tccagaaact gctatagaat
     9181 ttttatattc cctcatgatt tggcctaaaa aagtcgctct gttcattacc aatttatttt
     9241 tttctttagc agccacaagt tcttcattgt ctggatggac tgctgctgta taaacaacca
     9301 tatttacatc atctgatata ttatcttttt tctgaccaat ataaatagtt gccccttggt
     9361 ccctaagttt gtctaatagg tatgattcgt ttgaatccga acctgaaact tgataacctt
     9421 tattgataca tatttcagct aatgcactca tgctgatacc gccaattcct ataaagtgta
     9481 tattcataat aaaaccacct ttctttttaa ttaagtccta caatatgaac tatttctgtg
     9541 gtataataat ataatgttca atattttata attaatatgc atataatatt atatgtattt
     9601 tatttttgaa tataataaaa cataagtaat attataccac aataagaatt gttatgcatt
     9661 agttagttta acgttatata aaggtacttg taggaggaaa taataatgaa atttaaaaga
     9721 acagagagaa ttggagcaat agttaaaata ctgtctgata atccaaataa aatatatact
     9781 ttaagttatt ttacaaatca atttaatgca gcaaaatcaa caataagtga agatttatta
     9841 gttgtgaaaa atgtatttga gaagctacat ttaggtaaag ttataactat ctcaggagca
     9901 gcaggaggag taaaatatat acctaaaact tctatagcag aaaatgcaga gttcttaatg
     9961 gaattatgtg agagaatatg tgacaagtct agaatactat ctggtggttt tttatacttg
    10021 attgacctaa tttacgaccc tacaatagct gctaagatag gaaagatatt cgcatcaaat
    10081 attgagtatg tagatgcaga ttatgttgta acaatggaaa caaaaggtat acctatggca
    10141 cttatgactg caaaagctat gaatttacct ctagttataa taagaaaaga tataaaggta
    10201 tcagaaggtc ctacacttag catgacatat gtgagtggaa acagctctaa agttgaaagt
    10261 atgagtttgc caagaaaagc tcttaaacca gatagtaaag ttataattat agatgatttc
    10321 atgaggggtg gaggtactat aaaaggaatg gtagacctta tgaatgaatt tggtgcagag
    10381 gttataggta ctggagtatt catatctaca acgaatcctt cagaaaaaat ggtaaaagat
    10441 tatatttcat tgattcaatt ggatgttaat ggagataaaa tagtagtaga accaaactta
    10501 aaaactttta aagatgaata tagaaatgaa gatttagacg aagaagaaga ttcagaattt
    10561 gaatttgaga tagatgaaga ttaataaaaa aaatatttag aaaaatatgt aaaaataaaa
    10621 agaggatatc cctagttgtc catagaatta tttaaaaaac agtttatatt aatttattcc
    10681 agcgataata attttaatat aaattaattt ctgtcaaaat caaaggggga tattttggta
    10741 tgaagataac tgacgtaaga gtaagaaaat taacagaaga aggtaagatg aaatgtatag
    10801 tttcaataac tttcgataat ttgtttgtag tgcatgatat aaaagttata gaaggccata
    10861 atgggctatt tatagcaatg cctagcagaa aagtaggcga aggaaatttc agagatatag
    10921 ctcatccaat aaatgcagaa atgagacaag ttttagaaga tgcagtttta caagcatatc
    10981 atgaagcatt agttcaatgg gaagtagctg ctgaataata ataaatatat aaaatggaga
    11041 gagcccgaac atttaggctc tttttaaatg tagaatttct attaaaatat gtacttttta
    11101 gcaaaaaagt gctatattat tagtgataat gtatatgttg tatacaaatt gttagataaa
    11161 tgtattataa tgtatattga ttaagagaat aatacgaata aaattaaatt agattgaatg
    11221 gagtgtatca atgaacttta aagctataat ccttgctgct ggcaaaggaa caagaatgaa
    11281 gtctaaatac ccaaaagtta tacataaagt atgtggaaaa gaaatggtta accatattat
    11341 agatgtttca aaaaaatcag gggtaaaaga tactgtcgtg attttggggc atgaagctga
    11401 tgtagttaaa gaaaagcttg cagaagaaat tataatagcc atgcaaacag aacaacttgg
    11461 gacaggacat gctgtaaaga tggctaaaga atatattaat gatgaagata caatagttgt
    11521 actttgtgga gatactccac ttataaaaga agagacacta aagagattat ttgaatatca
    11581 tatagaaaat aaatatcatg ctacagttct tacaacaaga gttggaaatc caacaggtta
    11641 tggtagaata ataagagata agaagggtga ccttcttaaa atagtggaac aaaaagatgc
    11701 aaattcagaa gaaaagatga taagtgagat aaactctgga atttattgtt ttaatggaaa
    11761 gagtcttaga gaagcactag atttgctaaa taataacaat tcacaaggtg aatattattt
    11821 aactgatact gctaaaataa tgagagataa aggattaaaa gttggtgcat ttgcaggttc
    11881 aactattgaa gaacttatgg gcgttaattc aagagtagaa ttatcaaagg cagaagaaat
    11941 tatgagacga agaataaatg aatctcatat ggtaaatgga gtaactataa tagacactaa
    12001 ctctacctat atagaatctg atgttatgat aggcaatgat actataatat atccaggtgt
    12061 tatgttacaa ggaaaaacaa ggataggctc agattgtata attggaatga attcatctat
    12121 aacaaattca gaaataggtg atggtacaga aataaaaaat tcaactataa ttgatagtaa
    12181 ggttggagaa aattctactg ttgggccata tgcatattta agaccaaaga gtgacttagg
    12241 aaataatgta aaaataggtg attttgtaga agtaaaaaat gctattatag aagatggctc
    12301 aaaagcatct cacctttctt atataggaga tgcacatgta ggtaaaaatg taaatattgg
    12361 atgtggagta gtatttgtaa actatgatgg taaaaataaa tttaaatcag tagtaaaaga
    12421 caatgcgttt ataggttcaa attctaattt agttgcacca gtagttgtag aagaaaaagg
    12481 atatatagca acaggttcaa caataacaca tgatgtccca gatggtgctt tggcaatagc
    12541 aagagaaaga caagttatca aagaaggatg ggtagaaaaa aagaatcaaa aagacgacca
    12601 aagtaagtaa ttatgtagtt tattattttg gctaaaaaat ttagctcgaa attattataa
    12661 attatattca ttagtatttt aaaattttca ggagggttac ataaatgaat actagcggaa
    12721 gcgagattaa aattattgca ggtaattcat cgaaagagtt agcacaaaaa atagctgact
    12781 atataggtgt atcagtacta gattgtgaag taggtacatt tagtgacggt gaaatatgtg
    12841 taaacatgaa tgagacagta agaggttgtg atgtgtttgt agtgcaatct actaacagcc
    12901 cagtaaatga taacttaatg gagttattaa tcttaattga tgcgttaaag agagcatcag
    12961 caggtagaat aacagcagtt attccttact atggatatgc aagacaagac agaaaggcta
    13021 aggcaagaga tccaatcact gctaaattag ttgcaaattt aataactgct gctggagcag
    13081 atagagtatt aacaatggat ttacatgcag ctcaaataca aggatatttt gatataccat
    13141 tagaccactt actaggtgga acaatattag caaattattt taatgaaaag aaaatagaag
    13201 atttagtagt agtatctcct gacttaggaa gtgttactag atcaagaaaa tttgctaata
    13261 ctttaaatgg agaagttcca atagctataa tagataaaag aagaccgaaa gctaatgtat
    13321 gcgaagttat gaacttaata ggtgatgtta aaggtaaaaa tgttatacta ttagacgata
    13381 tgatagatac agcaggaaca atagttaatg ctgcaaatgc acttaaagag tttggagcaa
    13441 aagatgttta tgcttgttgt actcatggtg tgttatcagg tccagctatt gaaagaattg
    13501 ctaattcaga aataagtgag ttaatagttc ttgatactat acaacttcct gaagagaaaa
    13561 gaatagataa gataaagata aaaacagtag ctcctttatt tggagatgct ataagaatga
    13621 tattctctaa tgaatcagta agtaaattat tctaatttat tgtatttagg agagttgttt
    13681 aaaaggatgt aaaccttttg agcaactctt ttttgatttt atacttatga aatgctattt
    13741 ttctcttgtt agaaaggtat atattattgt agaaagaaat tatagtttaa aattatgtat
    13801 atttaagcaa aataaactta ataaagggca tattattcat aatattgtga atataaaaaa
    13861 tattacaaaa aagtattgaa aaaattcata ttatgtagta ttatgtaaat ataaataaag
    13921 aaagaggtgt ttccaatggg aaggttagaa gatgaacccc taataattaa atgattcagt
    13981 cacaatctaa agtgaatata aaaatctatt gaaagtttat ttataagaat tgaaaatagt
    14041 atctattcta tctttagtgt ttgtgagagg gttctggatt atataagatt actgtaaaga
    14101 agtaattttg aacaataagc atatctgtaa atgaagagtt tcgtttatat aatgattgtg
    14161 gctgaatcat aactataata gatagaatgt ttataataga tgattttata aaatactata
    14221 ttgtaatagc ttttagcttt caaataaata ttagcaaagg atggaaatga caatgataaa
    14281 actaaatgat aatttataaa tatctcctta cgatatataa taattgtagg gagatgtttc
    14341 tttttttgta tagtataatt ttttctagaa attctgtttc aatatgtaaa aagttggtat
    14401 atataaatag tataaactgt gttgctaaaa tatggtagat attatataat ataagtggat
    14461 atgtatatgc attatgagtt atataatttt gaatttaata aaatagaaac gttgatttat
    14521 gttctgcaat gtgggcacct tggagcatat tgagttagtg gtgcaaccgg ctatgaaatt
    14581 gtatttattt atagatacta ttattttcat agcttttttt atttaaaaaa tggttatatg
    14641 ttagaaataa atttggttag tacgtttaaa taattttact tagattttta accagagata
    14701 ttataagtaa gtattaatta aaaaatttaa gggggaataa aaatgaagtt aaaaaagaat
    14761 aaaaaaggtt tcactttagt ggaattattg gtagtaattg caattatagg tatattagca
    14821 gtagtggcag ttccagcttt atttagtaat ataaacaagg ctaaggtagc aagtgttgag
    14881 tctgattata gttcagttaa gagtgcagca ttatcttatt attcagatac taataaaata
    14941 ccagttacac cagatggtca aactggttta aatgttttag agacttatat ggaatctctt
    15001 cctgataaag ctgatatagg tggaaaatat aaattgatta aagttggtaa taaattagta
    15061 ttacagatag gtaaagatgg tgaaggagtt accttaacag aagcgcaatc agcaaaatta
    15121 ttgagtgata taggtaaaga taaaatatat acaggtgtta caggagataa ttttggagac
    15181 caattaaaag atactacaaa aatagataat aaagctctat atatagtact tatagataat
    15241 actgtgatgg actcaacaaa ataattaaaa ctatattttt ataaagaata ttgtaaattt
    15301 aatgaaaaat ataatataaa agacaggaag aaaatcttcc tgttttttta tactattttc
    15361 tttaatgttc aattatgaat gatgatataa ttgagatagt aaaggagtga aacctgtggc
    15421 taaaaaagtt agaataggag acaaactggt cgaaaaggga tatataacag aagaccagct
    15481 taaatgggca ctatcagaac aaaaaaacag tggaaaaaga ctaggagaat ttctagtaca
    15541 agaagggctt atagatagta acttactaat aagtgtatta aaagaactct tggatataga
    15601 aagtatattt ttagaaggga cagaaataga cactttagct acaaagatgg ttccagaaaa
    15661 tatatgcaaa agatatactg tatttccatt taaaattgat ggaaataaaa tatgtctagc
    15721 catgtcagac ccacaagata gagaagcagt tcaagatgta aggcgtatgt ctggtaaaga
    15781 cgtagagata tttatatcaa gtacagaaga tataaacaaa gcaataggtc atgcttatgc
    15841 acattcagaa ataaataagg caatgactga gtacaacaaa aatagaactg gtggagtaag
    15901 agaaacagta atacttgaag aagatgtaaa tgctgctcca atagtacgat tagtaaataa
    15961 catactagaa aatgctgtta gaatggaagc aagtgatata catatagaac aaagtgaaaa
    16021 ttacatgcgt gttaggttta ggatagatgg aatgttaaga gaatatatga ggatgaactc
    16081 agcaccatat aaggcagtaa tcagccgtat caagataatg tcagatataa atatatctga
    16141 aaagagaata ccacaggatg gtagaatcta tttaaaagta gataataaac ccatagattt
    16201 tagggtttct acaatgccaa ctaacaggga tgaaaagata gctatgaggg ttcttgataa
    16261 gagtaatttt atggttagta aagaagtact aggaatagac gaacatggct ctaagatata
    16321 tgatgaattg ataaacactc cttatggtct tatattggtt gttggcccta ctggaagtgg
    16381 gaaaaccact acattgtatt caatgttaaa tcaactaaac acagaaaata gaaatctatt
    16441 aacaatagaa gaccctatag agtatgaatt acctggtgta aatcaatctc aaataaatga
    16501 aaaggcaggg cttactttcg ccagcgggct tagagctttt atgcgtcaag acccagatat
    16561 aataatggtt ggagagataa gagatactga gacagcagaa atagcaatta gagcttctct
    16621 tacaggacat ttagttttaa gtacattaca tgcaaataca gcagttggag caatttcacg
    16681 tcttttagat atggatgtgg aaagtttttt aataacttca tctgttttag gtgtaatatc
    16741 tcaaagactt acacgaaaaa tatgtgagca ctgtaaggtt tcatatgaag cagatattgg
    16801 agaaaaaaag gctcttggta ttggtgtaaa tgaatcaata actatttata gaggaaaagg
    16861 ttgtgaaagg tgtaataata ctggatataa aggacgtcta ggtatatttg aaatgcttga
    16921 aattactcca gaaataaaag agttaataga ttcatctgca aatcaaagag aaattttaaa
    16981 gatggctaga aaacaaggta tggttagctt aaaagaggat atagttaaaa aagttttaaa
    17041 tggaaagaca acagtagaag aaatgattag aataattttg atgactgatt aaaggatgat
    17101 attatgaaaa gttttaaata cacagtaata aaagaggacg gaaaaaagca aactgatgtg
    17161 attgaagcca acgattttaa tgaagctaga aatcttctta gaaagagaaa tcttagagtt
    17221 attgaaatca aagaaagcaa aaataaaaat ataagattat ctagtagaaa gagaaaaaaa
    17281 gacttgggag ctgaccagat aagccacttt tgcagacagt ttgctattat agtatcttct
    17341 ggtataaata gtatttctgg attagaaaca ttagctagaa ggtcaaccaa cattacatta
    17401 agagaagaaa ttaatcgtat agtggcagaa ataaaagtag gttctactat tgcagattca
    17461 atgctttcat caaaatcaaa gtttccaaag ttactcggtg ctatggtagc tacaggtgaa
    17521 gctacaggta aattagatga agtattaaaa agtatggctt cattttatgc aagtgaacac
    17581 agagttaaac aaaagcttag aaatgctgca acatatccat taatagtttt gatttcttca
    17641 tttataatga tttttatatt tacaacattt atggtaccta agatgattaa ttcaattaca
    17701 acagtaggag caactcttcc acttataaca agaattgtta tggggtttgg tatgtttatg
    17761 aagaagtttt ggataatagt acttttagtt attatattat ttatatatca gtttaaaaaa
    17821 tatctaaaaa cgccagttgg aagggctcat aaagatagag ttataaacaa gatacctgtt
    17881 ttaggaaaag gaataaattg tatggtagct acacgatttt caagagcatt gtatctattt
    17941 gtatcaacag gatacccatt agttcaaggg cttgattata ttatagatag tgtaaataac
    18001 accatggcag aaaagatttt agtatcagct aaagatggaa taacaagagg agaaggttta
    18061 gcagaaaatt tagaaagata tacgtatttt gactctgtat tggtacagat gatagctata
    18121 ggggagcaga caggggaatt agagaatata agtagacaga tggccgaatt ttatgagtat
    18181 gaatccgaga tttatttaaa taggatggcc tcaatgatag agcctgtttt aataattgca
    18241 gttggtatta tagttgcgat actggttgta tcaatattta tgccaatgct tagtatatat
    18301 gatgctatgt aggagggaaa ggacaaatga acaaggttta tgtatcatat tatggcgatt
    18361 ctatttccat agttgagggc agatataaac acgataagga taagttttat gtaaaaaatt
    18421 ttaatgtgtt ttcaatagag gatattgcac aagatttttc taaggaaaag tatgctctat
    18481 tgagatacgc attagaaaat gttaagttaa aatcaagaca tgtagttttt tgtttaaaca
    18541 caagagatgt aattttaaaa ccccaaaaac ttcctaatat ggataaaaag gatttagacg
    18601 gatttatgaa gctagagatg gacgaaatga tggctttgga gtcagatgaa tacgtatttt
    18661 cgtatgaagt atctagtgaa atacctgata attcagatgg cgatgaatca agcttaaatc
    18721 ttattattgg agctatacaa aaggaagagg taaatgcaat tgtagagata atgcatgaat
    18781 ttgatttaat attagataga atagatacat tatcaacagc ttacttaaga atgcttaaaa
    18841 atttagatta caatgatata atggtaacca atataagtga caatagcaca attataaata
    18901 tatataagaa agatacttta tttattagtg ataatatacc tataaagata actgctaaag
    18961 atatggacat gcaattatta aatgtagtag aagaatcaaa aggtcttatg aattactatt
    19021 cgtcaagaaa ctttggtaag gttacggata ctatattaat attaggaaaa aggtattcca
    19081 ataaattaat ttatgaaact tttagctctg cattttcaag ctatgtttct caaggtctta
    19141 ctgagataat ggatatatct gatatgataa ctggagatat agatgaagat gatataaatt
    19201 caattgtaga gcttttagga agtatgatag aacctaaagg gaaaagagat tttgaaaata
    19261 taaacttttt accaatggat atacttagaa aacaactaaa agctaaacag atgaaaaaca
    19321 tactgaaaat tgctccaata gttattttga ttcttgctat accttattta tgtttaattt
    19381 tttcaaataa tttagcaaat aaagaacttg aaaatgttaa tggtgaaata agaaatattg
    19441 aatctgcata ttatcagatt gggaatatag aaaaaatgat acagagtaaa agcgaagaaa
    19501 taaaggtata taatatgtta attggaaaaa aggtaaagtg ggaaaattta ttggatgata
    19561 tagataaaaa tactccaagt aaggttgaac taacaagttt atcaacgact tatcaaaatc
    19621 ttggaactaa taataaagct gataattcaa aaagtaataa gactacagca gataaatcaa
    19681 gtaacaatga aataaaatca aatacgaaaa gtgatagtaa tggagataaa acaaataatg
    19741 atttaaatga agataagact acaaatgtgt cagaagcatt aactggaaat agtgagacta
    19801 atagtaataa cacagataaa gatgataaaa ctccattgta tgataagata ccaaatgtta
    19861 taaatataga aggaaatgct aaagactcct cttatgtagg tcaattttta tataaattaa
    19921 agggattgta ctatttcgag gatgttaaat tacatagcat aaaaagtaat gcagaagatt
    19981 ctaactatac atttagtatg acattatacc tgaaagaagg tgtattaact aatgaataaa
    20041 gatatattta aaaaagatat aaaagatata tttaaaatgg aagttagtct aaaagggatt
    20101 cttataaagg ttataatttt attagctatt ttatcatatg gttattttgg actttaccct
    20161 caatatcaga aatatattgc tacaaaaaat tctgtaaaac aagctactga aaaattaaat
    20221 atatataaag agaaaactgc acttatgcca aaatatgaaa aacaacttga agaagttgaa
    20281 agtgaattaa aagaaaaaag caaaaatctt ttatatgata tgcaagatgg tcttttttta
    20341 ataggtttag ataaaaaaat gaaggcttat aatattgact taactagtta tgacgttgga
    20401 aatgttagag aatacgaaca tttttatggt gtatcaacaa caataagtgt tagaggtgat
    20461 tatagaaatg tcaaaaaatt aatggcttat ttagaagaac aaaaaaatgt tactcaaata
    20521 ctagattata caatgacttc atatatagaa ccagaaaaag aagaggaaga aacaacaaac
    20581 aataaggatt taattaaaaa tgtgaacata aataaagata caaaaatata ctgggtggaa
    20641 ggcgaaaata agtggcattt atataaggat tgtccattga taaaagaggg agagattcta
    20701 aaaaatgaca aacttttaga taggtctact agttatctat gtaatacttg tgtacttaga
    20761 gctgcaacag aaaaagaagc aaaaaaagaa gtcgtagctc caaaggcaga aggtgtagta
    20821 gatgctacat ttaaatttat aatgtacact aaagagaata ctacaatgat acttgataca
    20881 gataatccat cttcatggag accgggaaaa tataatccat ttaagtctag tgcgaaataa
    20941 agctaagtaa gctttaaaga taggtgatga gtttgtacaa aaataatgaa aaaggtctta
    21001 ccttattaga agtaataata gctgttttta tactgacaat agttttaagc atttcttata
    21061 aagtgttcaa tggtataaca tcagcagtaa aaaggcaaca gattattaca gatgctcaag
    21121 taaatattaa tctaattaat aagtatctaa atagagattt ggaaaactgt aaagagctaa
    21181 ctaaaactgg ttcaggtaat aattatgagt acaatataga gatgccagat aatgttgtaa
    21241 aatatgaagt ttctatagaa actaaaaaac atacagaggt atattctgta acgagaatcc
    21301 aaaaaaatac aattgataca gaaaatgaag ttagagaaga gataatctat aatcaaccac
    21361 tagttcaaaa caataaagaa atgaaggaaa caccatttaa aatagaaaag caaactggta
    21421 aatctattta tactgtaagt atatactata atgaatcagt gcaagaaagt cataaaaata
    21481 tcaatttaaa taacaaaact tatacatttg atgttatgtc aagaataggg taataagaat
    21541 ttattaaaaa ataagtaggt tgataaaata tgagtaaaaa gaatagtgaa agaggatttt
    21601 ccttaattga agtgttagta gctatggcta ttatgggaat tgtactattt gcatttttta
    21661 atattatcaa tactaataac aaagcaaaca ctaaaaatga cacagatata acttccttaa
    21721 actatgtcca aagtgaaata gaaaatctaa gagaaaagat aaaaagtgga gaatttgatt
    21781 ttgatagttt agataaacta gaagatggaa ctgtcgtata tgaaaaatta atagataaat
    21841 caaaaaaagt tgtatatgat aaggttctta gtgagagtga cgtgagctta tatgatattc
    21901 catatgaaaa gattacaaca ataaaagatg aagatggtaa tttaatcgac aaagaaaata
    21961 ttacaaataa gataaagact attgtagaag ataaaagtgg tcagatatat aaagtagctg
    22021 taactgggaa aagcatgaat gactattcaa gtaaaaaaga ggttaaaatt gtaactgaaa
    22081 tatttaaaga taaataattt tacttggggg tgagattttg aggaagtgga ataaatttaa
    22141 aagtgaaaga ggggcagctt tagttttagt tttggttgta gtagcattgc tttctattgt
    22201 tggattgata ttttcaaatc agatagccaa tagaataaaa tctacaaaga ctactaatga
    22261 gggtatacaa gcaaagtatt tagcagagac atgtgtggaa aatagtattg ataaagcata
    22321 tgaaaaattg tatgatgagc ttgagaaaat ggataatgag tttaaaagtg aaaatcaaga
    22381 aaaatccata tctaggtcaa aattaagaaa tatatcagat gaagatttta ataatcaaga
    22441 tgaggaaaat attgaagcag aaagactagg atatatgaat aatataaatt tctatttaaa
    22501 taaagcttct tcagaattgg aaaaagctag tatggaattg aaaaagttat atgatttaga
    22561 tatgttggac tatagagaca ttgaatatgt agatgcaaat attatttcac atagagattc
    22621 tatcttagaa atttgcaaga attatacttc tggagatatt agtaaaataa atgaacatat
    22681 tctgaaagaa gatatagact caactacatt gattgaagca aagttagtaa ataatgatat
    22741 tcttttaaaa atgtttttag aagaaaataa aattgaaaat gaacatttaa attcagcctt
    22801 ttcacatact tataaggcgt tagataacat ttctttggca atgcaaaata tgattgagta
    22861 tagacataca tttcatatag atgagccaaa agtagaagta tctaatggga ttccaaattc
    22921 tcaagaatat tatgaactaa tacaaaatcc tataataaat agtatggagt atagctggaa
    22981 tagtaaatgg gatacattag aaaacctatt ggaaatttta ccaaatcaaa ctcaaggttt
    23041 taattcttta agagttcatt tgagaaataa tgtaagaaaa tttgagaagt taagcgataa
    23101 tatatctagt ggtaaaaaaa atacagcaaa aaatttctta aagtataaag agttattgta
    23161 tgaaatatca gaccaatgta atcaactaaa aagtatgtct tatgagaaaa tacctgttaa
    23221 atatgataac atggctttga taacaacttt tgactatata caaaatgagc ttttagcaga
    23281 gataaaatgt agattaaaag aattaaaacc ccaagaagtt gataaaacag aaggtattag
    23341 tataaaaata cctttttata aggctgacta cgacatgaca aaagaaggat ggcctaaatt
    23401 aaaagaaaat ggttcagggg cggagttaag cttaatggta actggtgata aagatggaat
    23461 aaaagaagta gaagttacag atagtaaaaa aaatattatt ggtttaggtg ttgaagaaaa
    23521 ttcaaattca aagtataagg ttgaggccat tgtaaacttt aatctaaata tagatacaaa
    23581 tgtagttgga aattataata taaaagataa aattttgatt aatcatgata taagttcata
    23641 caaaaaagta aactaaatat aaggagtaac ttagtataaa taaataataa aataatttta
    23701 ttatttacta ctaaaaaaat aagtaaacta atagaagata tggagagtaa atattatgta
    23761 tatttatgat ttattagagc aaggtataag actaaacgca tcagatatac acataacagt
    23821 tggaacaaat cctgtagcta gagtaaaagg tggatttgta aaactaagtg aacaaatatt
    23881 gacatcagaa gtaacaatgc aaatggcaaa agacatagca ggggaatcta tgtttaaagt
    23941 aatagaagaa catggagaag ctgatttttc agcttctcta aaaacaggag aaagatttag
    24001 ggtaaatgca tataggcaaa aaggaaatta cgcaatagca ataaggacta taacagcaga
    24061 aattccaaca tttgaaaaat taggtcttcc agaaaatata aaatctttta ctgaaaagca
    24121 taaaggatta gtcttagtta caggtccaac aggaagtggt aagagtacaa cacttgcaag
    24181 tatgataaac ataataaatg aaaaacaaca aaaacatata attacacttg aagacccgat
    24241 agaatatgta catcaccata aacaaagttt agtaaatcaa agagaagtag gaactgatac
    24301 agaaagtttt cattcagccc ttagagctat tttgcgtcag gacccagatg taatactaat
    24361 aggagagatg agagaccctg aaactgtttc aatagcatta acagcagcag aaacaggtca
    24421 tttagtattt tcaacactac atacagtagg ggcagctaag acaattgata gaatagtaga
    24481 catgtttcaa ccatcccaac aacaacaaat aaaaacacag ttatctacag tttgtgaagg
    24541 tgtggtatca cagcagttgc ttcctacagc agatggaaaa ggcagaatag cagcaattga
    24601 attgatgttt gctactccag caataaaaaa cctgataagg gaaggcaaaa catatcaaat
    24661 tccaaacatg atacaaactg gagttaaatc aggtatgaaa actatggacc aagatttaat
    24721 ggaactttat aaaaatggaa agattacaaa agatatggca cttagtaggt gtactgacca
    24781 agagtttatg acaagaatga taggtggagt aaactacaat ggatattata atcgttaatc
    24841 tatatgtatt tatagttggt atagtgtttg gaagtttctt taatgtctgt atttttagga
    24901 ttccagaagg tatatctatt tctagacctc cctcccattg tatgagttgt aatactagac
    24961 taaagccaat agacttaata ccagtgctaa gctatttaat gagtggcaga aaatgtagat
    25021 attgttatga gaaaatttct agtagatatg ctatagtgga attaattaca ggaattttat
    25081 ttttaacagt atttatgtta tatggaatat ctacatcaac tatttattat ttggttcttg
    25141 tgtctttact gattataatt acatttatag atattgatca ctttataata ccagataaaa
    25201 tactgatttt tggagctata ttttctataa tttttaacct tttatttaaa gaaattccag
    25261 ttaaggattc aattataggt gtttttattt gtggaggaag tgtctggatt atagttcttt
    25321 tgattgaatt tgtaataaaa aaagagtgta tgggtggagg cgatatcaaa ctatttgcaa
    25381 tgctaggtct ttacatgggt gttaaaaatg gtcttttgac agctttacta agcatttatg
    25441 tgggagcagt ctatggaatc tgtgtaataa ttacaagcag aataaaagga aaggaatata
    25501 attctgttat accatatgga ccttttatat ctattggagc attaataaca atcttatgtg
    25561 gaaagcaatt acttgaattt tatttttcta tggttatata gatgatagtc aagttaaata
    25621 aagttttaat ttaaaaaaat gaattaaagt atttaataag gacaatatta ttattaaccc
    25681 tattactaaa gtgctacaaa ctatggagta tataataata atttccttat tttttgcaca
    25741 tttgataaat aaatctctat gtgttttttg tgatagagaa aagaaaaatt tatataatac
    25801 gttttttatt tacataataa ctgtagtttt atctgtatta atatatctaa aatttgggat
    25861 tagtgtagaa tgtgtacaat atatggtttt aattccattt ttaataatta tatccattgt
    25921 agattactac acaatatatg tatatgatat aacaatagtt agtggtataa tagttcaagg
    25981 tataattttt ttggtctttt tcaatatagt tcaatttgat tatataaatc atatatttgg
    26041 actattgata ggctttatta taccttatat tattgctaaa ctaacaaaag gattaggcta
    26101 tggagatgta ggtctctata gcttatgttg ctttgcattg ggaaataatt atgggtttta
    26161 tgtgatagtt ttatcattta ttttagcgtt tttgtttttt attattttat acataactaa
    26221 aatatttaaa gatgtagata taagaaagca aattcctttt acacctttta tttccttgtc
    26281 aacaatttta ataatattta cagagtataa tatatttaat ttttattata gtttgttata
    26341 caaatttata taacaggagg taagaaatgt acgtagtagt agggttaggt aatccaggaa
    26401 agaagtatga gaagactaga cataacgttg gatttgatgt tatagatatt cttgctaaag
    26461 aatacaatat tagtgttact aaaataaagc ataaagcttt aattggtgaa gggagagtag
    26521 gtactgaaaa agtattatta gttaaaccac aaacttatat gaatttaagt ggagaaacat
    26581 taatagatat atataaatat tataaaattg atttaagtaa tatagtagta gtatatgatg
    26641 atatagattt agaagttgga aagatacgta taagaaaaaa aggaagtggg ggaactcata
    26701 atggcatgaa atctataact aagtgcctag gttcaaatga ctttccaaga gtgagagtag
    26761 gtgtatctaa accagaagtt ggtcaagact tagcagactt tgtactttct agatttagaa
    26821 aagaagaatc tgataacata aatgaagcat tagaaaaagc ggcatatgct attgactcaa
    26881 taataagaga aaatatagat atgtctatga acaaatacaa tggatagtga gagggagatt
    26941 taaatgaatg atgttttttt atatcctttg caaaattcaa aagagtataa agatataata
    27001 aactgtataa aaaatacaaa aggctcatta ttggttaatg gacttttacc agtacaaaaa
    27061 cctcatatat cgtattctat atttaatgat ttatcacgac aaatgatttt tataacgagt
    27121 agtgatttag aagcaaagaa agtatatgaa gatttaagct tttatatgga agataaagtt
    27181 gaatatttag gttttcaaga tatttatttt tatcatttgg atgcaaagga cagaaacgaa
    27241 gaagcaaaga aattaaaggt attgttaaaa ctagcaaata aagaaaaaat aattttagta
    27301 acttctattg aggcagtttt aagaaaatac ataccaaaac aagttttgct cgatagtgta
    27361 tctcattata aggtgggaga tagcttagac ttagaaaagt taactgagaa attagtttca
    27421 ttgggatatg aaagagtttc aaaaatagag gggtttggtc aatttagtat aagaggtgga
    27481 attattgatg tattttcact tgaatataca aatccaattc gtatggaatt atttgatgat
    27541 gagatagatt ccatacgaac ttttgacgta tattcacaaa aatcaattga taaattgcaa
    27601 caattctcta taactccatc tagggaattt atttatcctg aaaaaactac agatgcatta
    27661 gttaaattaa aaaaagaaac aactaaaaat actgatgaag gtacatttca aaatatagat
    27721 tatatatcga gtaagactta ttttgaaggt gtagagaact atatagatta tatttatcca
    27781 gaagaaaaca aaagtatatt tacttattta gcagatgatg caatagtatt tatcaatgat
    27841 ataacgagac ttaaagaaag atgcgaaaat tatataaatg agtttagaga aaattataaa
    27901 ttaaatcttg aaagaggtct ggctattaaa aaccaaggta aattgttata tcactataca
    27961 gatttagagt acttagtaaa agacaagtct gttgtattaa actcgctatt gccaaagtct
    28021 ataaataact tttctataaa atctataata aattttgaaa gtagagaagt tcctaccttt
    28081 aatggaaagg tagatttgct tgtagaagaa ttaaatcggc tgaaatataa tggatacaaa
    28141 ataattttag caacaaatac cttagaaagg gcaaataaat taggaaaaga tttattggac
    28201 aaaggcttag aaacgaccat ttctaaagat agagatattg aaattaaatc ttcacaagtt
    28261 ataattgtac ctgcacacat aaacagtggt tttcagtata aatctataaa gtttgttgtt
    28321 ataacagaca atgaaatgat aggtgtttat aaaagagcct ctaagactag caataaaaaa
    28381 gttaaaaagg gaaagaaaat agaatccttt ttagatttaa gcgttggaga ctatgtagtt
    28441 catgaaaata gtggtgtagg tagatacaca ggaattgaac aaattacagt caatgctatt
    28501 aaaaaagatt atatgaaaat agtatatcaa ggtggagata atctatatgt gccaatagac
    28561 cagatggaca aagtacaaaa atatattggt gcagaagtag aaaaagttaa gttaaataag
    28621 cttggaacaa atgagtggac gaaagcaaaa gcaaaagtta aaaaagaaat tgaagatatg
    28681 acaaaagact tgattgagct ttatgcaaaa cgtgaaaaaa tacaagggta taagttttca
    28741 aaagatacac cttggcaggc agagtttgaa tctttgtttc catatcaaga aacagaagac
    28801 caattaaagg ccatagaaga aactaaaaaa gatatggaat caagtaaggt aatggatagg
    28861 ctagtatgtg gagatgttgg ttatggaaaa acagaggtag ctattagaag tatttttaaa
    28921 gcttgtatgg accaaaagca agttgctgta ttggttccaa caacaatatt agcccaacaa
    28981 cattataata cattcaaaga gaggtttgaa aattatcctt tgagagtaga agttttaagt
    29041 agatttaaaa caccaaaaca acaaaaacag attattgaag atgctaaaaa aggattagta
    29101 gatattttaa ttggaacaca tagaataata tccaaagata taaatttacc aaacttaggt
    29161 cttgtagtca ttgatgaaga acaacgattt ggagtaaaac ataaagaagc tttaaagaaa
    29221 attaaatcta cagtagatgt acttacactt tcagcaactc ctataccaag aacattacat
    29281 atgtctttaa gtggaataag agacatgagt gttatagaag aacctccaca agaaagacat
    29341 cctgtaataa catatgttac agagagtaag gaaagcgtaa ttcaagatga gatagagaga
    29401 gagttaagta gaggtggaca ggtatttttt gtatacaata gagttgaaca cattgaagag
    29461 atggcaagta tgattcaaaa actcgtacca gatgctagag tggctgttgc acatggaaga
    29521 atgacttcta aatcattaga aaatataatc ctaggatttt taaataaaga ttatgatgta
    29581 ctagtctgta ctacaataat tgaaactggt atggacatat caaatgcaaa tacaatgatt
    29641 atatatgatg cagataaaat gggattggct caactttacc aattgagagg tagggttgga
    29701 agaagttcaa gacaaggata tgcatatctt ttgtatgaaa aagataaaac tttaagtgag
    29761 atagcagaga agagattaaa agctatcaga gaatttacag agtttggttc tggttttaaa
    29821 atagcaatga gagaccttga aataagagga gcaggaaata ttttaggttc tcaacaacat
    29881 ggtcatatgg cagttatagg ttatgatttg tacgttaaaa tgcttaatga tgcaattaaa
    29941 aaagtcaaag gtgaacctat tgtggaagaa attgatgttg aaatagattt aagtgtaaat
    30001 gcatacattc cagataatta tatcaaagat gagttgatta aaattgaaat gtataagaaa
    30061 atagcttcta ttgaaaataa agaagacatg ttggatatac aagaagaact tgaggataga
    30121 ttctctgata tacctaaacc agtccagaca cttcttacaa tagcttatat aaaatcgtta
    30181 tgtaagatat taaaaataga aaagataaga caattaaaag atgaaatatt attagttcca
    30241 ataacaaagt atagaactaa acagaaaata ggttataata tagttacaga attagaagaa
    30301 ttattagaaa agatgtgcaa ggtaaaataa cttgtaaatt taagaaggga tgttttaaat
    30361 ttgaaaaaag taataacatt ggtaataact atgattttgg tagtatctgt gactgcttgc
    30421 agttctagta aaggagaaac tgtggcaaca gttgaaggta caaagatatc atcagatgaa
    30481 tttaaaaaaa ctatagctct ttataaagat tctatggaac aaacttatgg aaaagatata
    30541 tgggataagg aagttgaaaa gggtgtaaaa tacaaggaca agtttaaaga tttaattctt
    30601 gaccaactta taactacaga agtaatatat tctcaagcta aaaaagataa cttacttcct
    30661 aaaaaagagg atgttgaaaa aagctttaaa gaattaaaag atgctatggg taaagatgaa
    30721 aaatataaag aacagcttaa gaaattaggt atagatgatg agtttttaag agaccaacaa
    30781 gagaaagatt tagcaatgca aaactatcaa agtaactttg ctaaaaagac taaaatatca
    30841 gatgaagaaa tgaaaaaata ctatgataca cataaggatg aatttaaaaa agatgaggta
    30901 gaagcttctc atattttatt gaaaacagta gatgataata acaagccact ttcagataaa
    30961 gaaaaagctg aagctaagaa aaaggctgaa gaggctctta aagaagttaa atcaggtgag
    31021 gattttgcaa aagtagcaaa aaaatattca caagatgctt cagcaagtga tggcggtaaa
    31081 ttaggattct tttcaagggg tcaaatggtt gctgaatttg aagatgctgc tttttctatg
    31141 aaaaagggtg aaatatctga tttagttgaa actcaatatg gataccacat aattaaagtg
    31201 acagacagaa taaatgagca aacatcattt gaagatgcta aagaaactat aaaagagcaa
    31261 ttgttgaaaa ataaatatca agagcaaata gaaaaattaa ctaaagaagc taaggtagaa
    31321 aaagatgaaa aagtaataaa taaaatcaca atctaagact caaaaaaaga gtaatatgct
    31381 taaaaaagct atatctggat aagatatagc tttttttgta tatttttctt aacaatggta
    31441 aaaataaact taaagtaatt tatggaggga ttaaaaaata tgaaagcaac aggtatagtt
    31501 agaagaatag atgatcttgg aagggtagtt atcccaaaag agataagaaa gacattaaga
    31561 attagagaag gagacccttt agagatattc acagcaaaag atggagaagt catacttaaa
    31621 aaatactctc caataggtga attaaatgaa ttttcgcaag aatatgctga aacattagct
    31681 gaagtactag gatatggagt gttgataaca gacttagatt ctataatagc tgtatctaag
    31741 cttcctaaaa aagattataa agaaaaaagt ataagtgatg agttagaagc aataataagt
    31801 gaaagaacag taaaatattc aaaagataaa aacattttac cattattcaa ggaagaccaa
    31861 aaagaatata ctagtcaagt tatcatgcct ataattagtg catctggtga ctgtataggt
    31921 tcaattgtag ttgtttcaaa ggataaagat acaattgcag aaagtgaaga aaaatcttta
    31981 aaaattgctg ctaacttttt aggaaaacaa gttcaataat tttatcataa agacctttta
    32041 catgaaggtc ttttgaagtt tttgtgtaaa atattgaata tagcattaaa aatatggtat
    32101 attaaagttt gttatataaa cagtatggag gttatgttat gaacaataac aaaaataaag
    32161 actcctttct aaaaggagcc cttatacttg ggctagcagg tgtaatcgta aaaataatgg
    32221 gtggattttt taggattccc cttggaaaca tgattggttc agaaggaatg ggatactatc
    32281 aggctgctta tcctgtgtat actctatttt taacattagc tactgctgga tttccaacag
    32341 ctgtggcaaa attggtttcg gaaaaagttg ccattggaaa ttttaaaggt gcaaataaga
    32401 tatttaaggt atcacataca gttttattca ttactggtat aatatcattt tgtatattat
    32461 tttttggagc cgactacata gtaacaaacg taatgaaaaa tccaggtgca ttatactcta
    32521 tgaaagccat tgctccagca ttactatttg ttcctgcaat gtctgcatat agagggtatt
    32581 ttcaagggag acaagatatg actaagattg ctgtgtctca agttgcagag caatttttta
    32641 gagttgtatt aggtcttact ttggcttact tgcttatgaa gagtctaggg cagacttttg
    32701 gtgcagcagg tgctatatca ggggctacaa taggttcaat agcatctatg ctttatttgg
    32761 tgtttgctta tatgctaggg agaaaagagc gtagagcaga gattgatgca agccaaagat
    32821 ttaaagatga aagagtgtct tatattttta aaaagctttt aactgttgcc attcctataa
    32881 caataggagc atcagtaatg ccacttgtaa atatgataga taatgttatt gtaataagaa
    32941 gacttatgga agcaggcttt acatataaag tggcaaattc aatgtttggt caattaacag
    33001 gtatggcaat ggccacaata aatttaccag cagtcataac aactgcaatg agtatgagct
    33061 tagttccagc aatttctaaa gcatatgcat taggaaataa gtcaaaagct agaaaagata
    33121 ctaagagtgc agttaaggtg actttactta tagtattgcc ttgtgcgttt ggtatggctt
    33181 ctttagcaat accaataatg ggtcttttat ttccacatga accatcttct gtaggaacta
    33241 ttttgtttac tcttactcca tgtgtattat ttttaggatt aatacagaca ttgactggta
    33301 taattcaagg aatgggaaaa ccaatagtac ctgtaattgc tttatgtgtt ggtatgctat
    33361 gtaaaattgt aataagttat actttaacag gtataccaga tattaatgta ctgggttcag
    33421 catttggtac agttacagca tactttgtgg cagctatgat taacttacta tacgttaaaa
    33481 aacacatgaa tgtaaacttt tctaaaaaag aatttataat aaagccattt ataacagtta
    33541 tgacaatgtt tataatggtt aagttgagtt atggtgcttt agttggattc ttaggaaaca
    33601 gcatatctac aataattgca atatgtgttg gtggaattgt atatgtagta gtaatacttg
    33661 gaataggtgg aattaaaaaa gaggagattt taactatgcc aaaaggtgat aaattatata
    33721 aattgttaaa aaaagttaaa cttataagat aatataggaa tatatatgca aataaaggaa
    33781 atactataca aatagtaaat ctccttattt gtataataat ataaaaataa agtgaaattt
    33841 gctaatagga gtgatgttat gggtaagata agtattgtag gtcttggacc aggagactat
    33901 tctcttataa gccaaggtgt gttagaatct ttaataaata gccctaaagt tttttttaga
    33961 acagagaaac atccaataat tgataaactt aaagaaacta tacaatacac ttctttagac
    34021 tacttttatg aaaaagagga aaattttgat aatgtttatt tgaaaatatc tgaatttatc
    34081 atagaagaat ccgcaaaggg tgacctagtg tatgctgttc ctggtcatcc aagagttgca
    34141 gagaagactg taagtataat agagagatta gctaatgaaa agcacataga agtagagata
    34201 ttgccctcta tgagctttgt agacgctatg tttaactatt tggctataga cccatctgaa
    34261 ggatttaaac ttgtagatgc ttttgaaata gaaaattcat atatagactt ggatacaagc
    34321 atgataataa ctcaggtata tgataaattt atagcatcta atgtaaagtt gaaacttatg
    34381 gaacattatg actataatca agaagtatgt atagtaaatg gagcaggtgt caaagattta
    34441 gaaagtaaaa agtttataaa gctttgtgaa ttagacagaa atgaaaactt ttttgactat
    34501 ttaactagct tatatatacc caaaagttct aaaaagatgt ataatacagt acatgactta
    34561 gaaataatag ttaatacact tagaagtcca tctggatgtg aatgggataa aaaacaaaca
    34621 catcaatcat taaaaaattc tgttatagag gaagcatatg aactttgtaa tgcaattgat
    34681 aataatgata tagatgaaat ggtagaagag ttaggtgatg ttcttttaca ggttatattc
    34741 cattgtcaga taggaaatga ggagggctac tttgatttaa aagaagtagt aaatggcata
    34801 tgtaaaaaat taattcatag acatcctcat atatttagtg gtgcaaaact agatatgtct
    34861 aagtttgaaa aaacttggga agaattaaag aaagaagaga aaggcgaaag tactataaca
    34921 gatggcctta gaaggatacc aaaacattta ccagctctta tgaaggctag taaagttcaa
    34981 cataaagcag cacttgtcgg ctttgattgg gacaatgtag aagatgtatt taaaaaaatt
    35041 gaagaagaat atcaggaatt acttgacgaa cacagggtcg ggaatataaa atacataaag
    35101 gaagaactag gcgatttatt attttcgata gtaaatctag ctagattttt agacatagac
    35161 tcaggagaag ccttgaactg tactacagaa aaattcataa atagatttga ctttatagaa
    35221 aaaagtgcaa tgaacttgaa taaaaaaata gaagacatga cacttgaaga gatggatgag
    35281 ttttggaatc aggctaaaaa acaataaaat tgtggaagga aatttgtcaa aaatctagaa
    35341 cttttatcac aaagatagaa gtctatatta taaacatttt taggaggtaa atatcgtgaa
    35401 taaagctgaa ttagtatcaa agatggcaga aaaaagtgga ttaacaaaga aggaagcaga
    35461 agctgcatta aacgcattta tgagttctgt tcaagatgca ctagtaaata atgaaaaagt
    35521 tcaattagtt ggatttggaa catttgagac aagagaaaga gctgctagac aaggaagaaa
    35581 tccaagagat ccagagcaag ttatagatat accagcttct aaagcaccag ttttcaaagc
    35641 tggaaaagga ttaaaggata taataaatgg ataattagaa atcttgtgaa aaagtgtgga
    35701 taaatttgcc acactttttc tatagagcca aggaaggtga taattatgag attagataaa
    35761 tttttaaaag tatctaggat tataaaaaga agaactgttg caaaagatgc atgtgataaa
    35821 ggaattgtca ctataaatgg aaaagttgct aaatcttctt cagaagtgaa tattggagac
    35881 acattagaaa tacaatttgg agagaagaag atgaagttta agataaatga agtaaaagag
    35941 catgtattaa aaaatgatgc aaaagaaatg tacgaaattt tagaataaaa ttaatagtcc
    36001 tctagtatat taatagtagt attattgaaa tatttactgg ggggatatta tggaacagaa
    36061 cataactttg aaagatagat caaagctggt tatttcggga gttgagcaca tatactcttt
    36121 taatgacaaa agagtagagc ttaaaacttc tgtaggtgag atggtaattg aaggcgaaaa
    36181 cctggatatg agcaagctaa gtatagacga aaatataatt agcatagatg gaactataaa
    36241 ttctatggta tatgcaaagc ctaaaaagcc acaagagagt ttcttgaaaa aggtatttag
    36301 atagatgata ccttttactc aagatgtaag tattttttat gctactattt atggtggaat
    36361 actcataggg gtgttgtttg atttttatag agggctaaga ggaaatttta aattcatcaa
    36421 ttattttgct ataatctttg atgtattgtt ttggtttcta gctacggtta taatattcgt
    36481 aacaataaac cttacagaat tttttgattt aagatattat cattttgtgg ctttgtttat
    36541 tggttttatt ttatactata acaccataag taaaattgtt ttatcaatta tcaataaaat
    36601 aattcgtttt gtgagaaatt cttttaaaaa agttacacat tacatagtca gttttttaaa
    36661 taatttgtat tacgttataa tctattcact acacctacta tttgatatta tattttatat
    36721 accaaacata tttatagcaa caagaaaaag tataaaaaga aaatcaaata aaaaattaaa
    36781 gaacaagaaa aaatcaaaac ctaagaagaa aaaaaataaa actaaaaaaa gggtgtagga
    36841 aaaatgaact taagaaaaaa attttcagga cagattattg ttatcagttt atttttagga
    36901 atatcaattt ttagtatgat gacaggattt gtatttgagt atacaaaagc taaggaatat
    36961 aaaaaagaaa tagcaagtct aaataaacaa ttaaaaaaga ctgaaataca aataaacaac
    37021 ttaaagaaag atgaaaaatc atatgaagga gacttagagg atatagcaag aaaaagattg
    37081 aatatggtaa aaccaaatga aacagtttat gtagacataa atcgttgatt aaaacttgct
    37141 aataagttta cagatgtatt gattttaaaa tttaagattt aataaaaaag tcattcagtt
    37201 aaggatggct ttttgttttt attgaggagg gaatttcata atgaaagtag gagctataga
    37261 tattgggaca aactcaatga gattactgat agcagattat agagatggga attttataaa
    37321 cagaaaaaaa cttgttaata caactagaat tggttatggt gtggatagta aaggatatat
    37381 aaaagaagat gctatcaaaa gaaatataaa agcattaaaa gaattttctg ataagtgtag
    37441 tgatgaaaag tgtgaaaaaa tttattgtat ggggacatct gctcttagag acagtaaaaa
    37501 tggagattac tttgttaaat tagctaagga agaaactgga atagatgttg atatagttac
    37561 aggaatagaa gaatcaaatt tgggctttaa gggtgtttta gaaggaatac aaggtgatga
    37621 tactattatt gttatagata ttggaggagg ttcaacagaa tttattgtag gtgattcaga
    37681 aggaattaaa tttaataaaa gtgagaatgt aggtgctctt aggatgacag agagattttt
    37741 gaagcacgat attattgaag atgaagagtt tgaagctatg agttcattta tatatgatga
    37801 gattgacaaa acgttaaatc atataaaaga cagaggatta aaaactctta taggtatagg
    37861 aggtacgatt acatcattat cagcaatgaa tcaagaatta gaggtttatt ctatggaaaa
    37921 aatacataat agtaaaatac acaaaaaaga aatagaagaa attctacaaa aattaataaa
    37981 gatgacatta agtgataaaa agaacttaaa agggctacaa gaaaaacgtg cggatattat
    38041 aactgctggt gtaaaaatat tgaatataat aatggaaaaa ttagaaatag aagaaataac
    38101 cgtaagtgaa tacgataatt tggaaggctt aatatgtcaa aagtcaaaaa aaatgtatta
    38161 atattttttg attttaaaca ccgattatga caaaaaaaat tactcctctt tgttacaatt
    38221 cttaatataa acaaaagagg aggttttttt atgcaaagaa gtgtagcagt tgtgaagaat
    38281 aagactgtaa aaccaaatgt cataaaatta ataagcggat atattgattt tactacggcg
    38341 gcattcgctg tgatgggatt tttacttagt aggtctatta tagtagactc tgtagctcct
    38401 ttaggaattg ccttttttat atgtgcatca aagattgata agtacaagat acctgtattc
    38461 atatcaacac tattaggaat attgttatca tctaatgatt ttggaaatgt agcaaaatat
    38521 gctgtgtgcc ttacaatatt tatggctatg aataaaaaat taaaattatc agattcaatt
    38581 gctaaggttg catttatagg tgcaataata ctacttccaa tctccatggg acaagctttc
    38641 ttttcaaata ggtatgtgta tggtcttctt atagcaggag tagaagcaat aataatgttt
    38701 atatctatat atgtgttttc ttatggaata ggtttaatgg ccaatgtcaa caataggatg
    38761 tctgtacgga cagaagaagc tatatcaata agtttactgc taacctttag cataatggga
    38821 ataggagata tatccctgtt tggaatatca ataaggacca ttttatcgac cgtattgata
    38881 ttgctgacct caatagtagg tggagctacc atgggagcat ctagtggtgt aattattggt
    38941 gtagcatcca ttttaaataa tatgacatca gctatttata tggggatata ttctttttca
    39001 ggattaataa gtggagcatt taataaaata aataaatatt tctgtatact aggatatatt
    39061 ctaagttgga caataatata cctatatact tctggtatta cttctaatat gatgcaatta
    39121 agagatatac tattaggttg tttgatagta ctagtattgc cagagagatt atttaataag
    39181 attgaaaaat taataaaaag taatgtagct tcaaatgaaa tcgtatatga ttatattatg
    39241 agaagcaaaa acctaactaa tagcagattg aacagtattt ataaaactta tgatgacctt
    39301 gcagatacat ttgataagat aagagaaaaa gacaaagtgc tagaccaaag agatatagct
    39361 aatgtaatag atatgattca taatgatgaa tgtaaaagtt gtagtatgag aagaatgtgc
    39421 tgggagtcaa gatttaatca tacatacact atgatataca acattcttga aaaaattgag
    39481 gagaaagggg agttaagcct aaacgatata cctaaaaatt ttagaaagga atgtatgaaa
    39541 ccagagtcaa tagtgaaaat atcaaatcac tattacaaaa tgtttgtatt agattatgat
    39601 tggagtgtga agttttcaga aagtagaaag ttaatagcaa atcagataag aagtatttca
    39661 aaatccatta aatcgttatc tcaagattta gaaggcgaca taatgctaga tatagaaaag
    39721 gaaaaaaata tatatgaaca gttagaaaga tatgatataa cagttgataa agtaagttac
    39781 ttaacaaaaa gtaacagtga gtttgaaata tcaatagaaa agaaaacttg ccatgatgga
    39841 tgtatgtgtg aagataaaat tgtaaatata atttcagacc tagtaggtga aaatatgtct
    39901 gttagaaaaa tagggtgcca ttgtttagga ggtaaatgta aggcaacatt cgttaaatcc
    39961 caaaaatata aggctgttac agaagtttca gcaatgtcaa gggatgggca tattctttgt
    40021 ggagacaatt acacatacat ggagataaat gatggtaaat acatgatggc tataagtgat
    40081 ggtatgggaa aaggtaaaaa agcatatgaa gaatcttctg ccactattga tatactggaa
    40141 aaaatgatag atgcaaaaat taaagatgag atagttattg atactataaa taatatgttg
    40201 ttgttaaagt cttcagaaga gatgttttca actctggatt tgggaatttt agatttaaaa
    40261 cgaggttgtt tagaaactat aaagatggga gcgtgttcta cttatataaa aagagaggat
    40321 ggagaagttg acttaatatc gtcatcatca ttacctgtgg gtatattatc agatgtaaaa
    40381 attgatagaa aaaatgtaaa agttaaggaa ggagactatg ttataatggt ttctgatgga
    40441 attgtagatg caggaagaaa taacaattta ggagacaatt ggttaatata cttcttaaag
    40501 aatatagaga ctacaaatcc aaaggaaatt tcaaatttaa tattggatag agcattagag
    40561 ttacaagcgt tacaaatcga agatgacatg actgttttag ttacgaaaat atgtacaaaa
    40621 taagggttac aaaattatgt gtataagatg tggataactt gtgtataata tgttaataga
    40681 cttttgaaaa aagtatagtt attaacaaaa ataacatata aatcaacaaa atttgttgat
    40741 aagtatggca gaaatgttaa taaaatgtaa aaaaagaaaa aacaaaagct attatcaaaa
    40801 gagattcatg ttgaaataac taaatttcta agtaaagaaa acctgtgaat atgttgatta
    40861 ttatgtggat aactattaat tcgatattta tttatatagt atgaataatt gaataatata
    40921 tctaaaaaaa gccatcctat tattaagtaa acttattgaa aggatggctt tttatgaata
    40981 gaaaattgtg caaagcaata atacctttat ttatttctat tttttgcatg ttaataataa
    41041 gtataaaagc tgaccaagga tatcaaaaga ataatgatag agcaattact gtcatgaata
    41101 ataatgatac tgaagatgat gctgaaaaaa aacaagaaat agactatgaa aaaacatgtt
    41161 ggaatttgca atctttgttt aaaagtgatg accaatggaa aaaagaactt aagagttttg
    41221 ataaagatac taaagagttg aaaaattaca taggtaaagt aacaaaatcg ccaacacatt
    41281 tgtcttttgc cttaaacatt aaagaaaaac ttgatataag actaaataaa ctttctgcct
    41341 atgtaaaatt aaaacaagat actaataaaa attcatataa gtacttagat atgaatgata
    41401 gtatgagtaa atcctatggg aattatatgg gaatttgttc agatttagag ttagaaatac
    41461 taaaattatc aaacaaagat tacaagaaaa ttatttcaaa taaaaatata aataggaaat
    41521 atggtgcata tttaagagat ataagaagga ataaagtaca ttatttggat gataaatcag
    41581 aagatattct aagtaaagta tcttcaataa gtagtcttcc aagccaagta tatgaattat
    41641 ttagaaatat ggatagaaaa actaatttga caccagctca atatgcaagt gaattagagt
    41701 catcagatag agaaaataga aaaaaagctt atcaagatga acttataact tacaatgata
    41761 atataaatac aattgcaggt ttaattactg gtcaagttaa aaagaatatt ttttattctc
    41821 aggaaagagg ctatgagtct tctttagaaa tgtacttgga atctgataat gtagatgaaa
    41881 aagtatacaa taatttgata aggactgtaa atagtaatat ggacagtcta cacaaatata
    41941 tagatttaag aaaaaaagta ttaaaattag ataaggttca ttcatatgat atgtttgtac
    42001 ctatggttaa ggctgttgat aataatatta attatgaaaa ggcacaaagt atagtatact
    42061 cagcgttgtc acctttagga aaagaataca atgatgtagt atataaagct ttcaatgaaa
    42121 aatggataga tgtttattca catgaaaata aagtaagtgg tggatactgt ttatctgttt
    42181 atgaaaatca tccatatata cttttaaatt ataataattc attaggttca gtttcgactc
    42241 tatcacatga gcttggtcat gctgtatacg aatacttaag ttctaagaat cagaattatt
    42301 ttaattcaaa tccgtctata tttactcacg aagttgcatc tacaacgaat gaagcattac
    42361 tttatgagat gcttataaaa gaagccaaag gaaatgatga aaaagcttat tatataacac
    42421 agtatttaga cttaataaaa gatactcttt atacacaaac tatgtatgct gaatttgaaa
    42481 aaactataca tgaattggta gaaaaaggaa aaagcgtaaa cgcattagta ttaaatgatg
    42541 cttggggaca acttcttaaa aaatattatg gacaaagcta tgaattagac caattatcta
    42601 aggtcggatg ggctagaatc ccgcattttt ataatagttt ctatgtctat aaatatgcta
    42661 caggttgtag tgcaggagta agttttgccc aagacatcct aaagaatggt tcagataact
    42721 atatgagctt tcttaaaaag ggaagttcag attatccaat aaacttgtta aaatcaagtg
    42781 gtataaattt aactaataca aaacccatag aaaatacaat caaaaaattt gatagtttag
    42841 tagcagaact tgaaaagcta ttataaacat tattgtaatc cacatggtaa atatatcgaa
    42901 ttaatgataa aatatactaa aagtttaaag tcctatcaag ataggacttt ttatttatta
    42961 attagtggga gatgattcta tgcaagttaa agttaaaaag gctgtaatac cagcagcagg
    43021 tttaggaact aggtttttac cagctacaaa ggcacaacca aaagagatgt taccaatagt
    43081 tgataagcca actctacaat acataataga agaagctgtg gcctcaggaa tagaggaaat
    43141 actaataata acaggtagaa ataaaaaatc tattgaagac cactttgata aatctgtaga
    43201 attggaactt gatttagaga aaaaaggcaa gaaagaattg ttagaggtag tacaaaacat
    43261 atctaacatg attaacatac attacataag acaaaaagag ccaaaagggt tgggagatgc
    43321 aatttattgt gcgaggcatt ttataggtga tgagccgttt gcagtaatgt taggagatga
    43381 tattgtagat aatgatgtgc cttgtctaaa acaactaaca gatgcatatg aggaatatag
    43441 aactacaata ttgggtgttc aaaaggtaaa tcaagaagat actaataaat atggtataat
    43501 agaagcaaaa aatatagagg gtagagttta taaggtaaaa gatatggtag aaaaaccaga
    43561 gtcaggaaaa gctccttcaa atattgctat tttgggtaga tatataataa ctcctgaaat
    43621 ctttgatatt cttaaggatt taccaccagg taaaggtgga gaggtccaac ttacagatgc
    43681 tttaaaaata ttatctaaaa aagaagatat gtatgcatac aattttgaag gtaagagata
    43741 tgatgttggg gacaagctag gatttttaga agctactgtt gactttgctc taaagaaaga
    43801 agaccttaag gaagatttta taaaatattt gaaacatgta tgtagtgaat ttgataaaaa
    43861 taacaatgtg ttatacaata atacagatga aaaaatagat ttaattgaag ccaatggagc
    43921 tagagaactt gaagttcaaa aataattaat gaaaggtgat gtatggtggc aaaatcaagt
    43981 cagaaaagaa ttaatagaat aatagctgtt gggattgtgg gaatcctcgt attaacgagt
    44041 ataacagctg ttttgagtat gattgcagct atgtaataag ataaatgaca gcagttgata
    44101 tacataaata taaaaaccat atataacttg atgtagttaa aaacttacaa tattttagtt
    44161 atatatggtt tttagaattt ataaatgtaa aaaaaaattt ttataatagt tgcatatttt
    44221 aaaggaaaat attattataa gatataaata tagtgataaa tttataaaaa cgtgctaaga
    44281 ttggacttga aatacagtaa tatagtaggt aataatgttt taaattttat ttattgacca
    44341 taatatataa ataatgatat tattagactt aactgtttaa ataaaacttt tctttttcat
    44401 gccttcaatt tccaaaataa gtatgtatat aaatgaatat atagatgagg aggatttttt
    44461 tgattttaga tgataaagta ttagaaggat taggtagtaa gaccttagtt gagctacgag
    44521 agatagctaa agaattaaaa ataaaatcaa ttactacata taagaaaaat gagttaattg
    44581 agattataaa ttccaaaagt aaaaatgagg aaaaagtaaa cgaagagact aaagaaaaaa
    44641 acgaagatat taaagaagac ataaggaata gaaaagaaaa tatagagcgt gaaagtatag
    44701 aaaaagaagt tgaatataag agccctacca aatcatacaa caagaaagaa atagataccc
    44761 aaaatatttc ccaaaaccaa gaaaatagaa atcaaagaaa tgactataat aagacaacga
    44821 gtgtaaatga tagtaataag tcatcaaata tatctaatag aatggtcaga aataataata
    44881 aaaactatta tatgcctaaa caagttgatg aatctaagat agtagatgaa tttaacacat
    44941 caaaagagga tgaagtagta ggtgtacttg aaattctgcc agatggattt ggttttttaa
    45001 gaggttcaaa ttatttgtct actgaaggag atgtttacgt ttcaccatct caaataagaa
    45061 gatttaatat gaaaacagga gacaagatta agggaataac tagacatcca aaaagtggag
    45121 agaaatttag agcactttta tatgttcaaa aaataaatga tgaaaaccct gacacagcta
    45181 tacaaagaaa tgcgtttgag acattaacac caatatttcc agaagaacga cttacactgg
    45241 aaactaatag aaatgaaata gctacaagaa ttatagattt aatttcacct ataggaaaag
    45301 gacaaagagg attgatagta gcacctccaa aagctgggaa aacagttctt ttaaaaagcg
    45361 ttgcaaatag catagctaaa aatcatccaa atgttgaact tatagtactc cttatagatg
    45421 aaagaccaga ggaagttact gatatgaaag aatctattga aggagacgtt atatattcca
    45481 cttttgacca agtatcaagt catcatgtca aggttgcaga gatggtgtta aatagagctc
    45541 aaagattagt tgaacatggt aaagatgtag taattttact agatagtata actagattgg
    45601 caagggcata taaccttaca atatctccta caggtagaac tctatctgga ggtattgacc
    45661 ctggtgcact tcatggacct aaaaagttct ttggtgcagc aaggaatatt agacaaggtg
    45721 gttctttgac aatattagga acagcattgg ttgaaaccgg ttctaggatg gatgatgtaa
    45781 tatttgagga atttaaaggt acaggaaata tggaactaca tttagataga aaattagcag
    45841 aaaaaagaat attccctgcg atagatattt acaagtctgg tacaagaaga gatgatttat
    45901 tgcttgatga tgaagaaaag actgctcttt ggagattaag aagagaaatg agtaataatt
    45961 cagtaatgga aattacagat aaagtcatcg aacttataaa gagaactaag gacaataaag
    46021 agtttgtaaa atcaataaag aatttgtaaa atcaataata caaatgcaaa tgtatgttat
    46081 ataaactaat agaaaaatca cttgaatggc ataataagtt gtgctataat aatgtagttg
    46141 ataatgtaaa aagaaagtta atataattaa tttttttaaa taaagagaag aggtgactat
    46201 aatgcaaaag gaaatacaac caaaatacaa tccagttgaa gtgcgttgtg cttgtgggaa
    46261 cacatttgtt gctggttcaa ctaaggacga aataaaagtt gaaatatgtt cagagtgcca
    46321 tccattctat acaggaaaac aaaagaacat agaaaaaggt ggaagaatcg acaaattcaa
    46381 gaaaagattc aaaatggact aatttttata aaagtggagt tggaggaagc caactcctat
    46441 tttattacaa aaaaacattc atattctact ttaaattgtc tttttgtatt ataataataa
    46501 atatgtaaaa ttgagtattc taaatacaat aaaatgaggt gagtggatgg caaaacaagc
    46561 tgtaggtggg caagctgtta ttgaaggtgt aatgatgcag tcaaaagaca aaagagcggt
    46621 agctgttaga aaaagcgacg gagaaatagc actaaaagaa gatagaataa aaagttgggt
    46681 aagagataag aatatagata agataccttt tattagaggt tcttttgtta tgatagatac
    46741 aatgattcaa ggcataaaaa gcttaaactt ttcttcagag ttttttatgg aagaagctga
    46801 agaggataaa tttgatttat ttataaagaa aatttttaaa gataaggcaa atgatataat
    46861 tataatattt tcattggtca tagcaatgtt actttctgct ggattattca tttttatacc
    46921 tacattggta ggaggagctt tttctaaagt aatgcctaat gattttatgc ttaatttaat
    46981 agaaggaatc ataaggatag ctatattatt tgcatatata gtattgattt cacgaagtaa
    47041 ggatatagaa agagtctttc aatatcatgg agcagaacat aaatctatat attgctatga
    47101 aaatgattta gaacttacag tagagaatgc tagaaaattt aaaaggttac atcctagatg
    47161 tggtacaaat ttcctattta tagttatggc tgtatcaata atattgtttg cattttttgg
    47221 ttggccaaat ccaatcctta gaatatttat gagaattata tgtgtgccta tagtagcagg
    47281 tttatcttat gaagtaatta gagttcttgg gaaatatgat aatggattta ctaaaattat
    47341 tgcatatcca ggaatgatgt tgcaatattt tacaacaaaa gagcctgatg atgagcaatt
    47401 agaggttgct ttggaagcat tgaaggcagt agtagattaa agaaaaggat atttgttatg
    47461 acaataaaag atataattat taaatattca gataaactaa aagatataag tgatactcca
    47521 agattagata cagagttatt gttacaaaaa acattgggtg ttgataggtt atatattcac
    47581 ttaaatctga ataaagagct aactgaagaa caaaaaacaa aatttatagg ttttgcagaa
    47641 gaaagattaa atggaagacc aatagcatat atagttgaaa atagggagtt tatgggatta
    47701 gatttttttg ttaaggaagg cgtattgatt ccaaggccag atacagaaac tttagtagaa
    47761 gagataattg aaatatgtag agaaaaaaaa gatgtaagta tattagatat aggaactggt
    47821 tctggagcta ttacaataag tttagcaaag tacattgaaa attctaaaat catgtctttt
    47881 gatgtatcag agatagcatt agaaatagct aaaaaaaatt ctattataaa tgaagttggt
    47941 gaaaaaataa agtatattaa ttctgattta tttacagcaa taagtgatag taacataaaa
    48001 ttcgatatca tagtttcaaa tccaccatat attaaaaaac aagatataga aactctgcac
    48061 aaacaagtta aagattatga gccatataat gctcttgaag gtggagaaga tggattagac
    48121 ttttatagaa gaataacaga acaaggtaag aaatatttaa ataagcgtgg aatattagca
    48181 tatgaagttg gtcataatca agctgaagat gtaataaata ttatgaaaag taatggatat
    48241 aaaaaaatat acacaaagaa ggatattcaa ggcattgata gagttgttat aggttacaat
    48301 atatgatata atcaacaatt gatatgttac atttaatcga ggtgaaagta tgttaaaaaa
    48361 attagaggta ttggaagata catataaaga cttaagtgaa aaaataggtg atccggatgt
    48421 aataaatgat caaaaagttt ggcagaaata tataaaagaa catgctgact tagaacctat
    48481 tgttatgaaa tacagagagt ataaaagcgt tttagatagt ataaaagaat caaaagaaat
    48541 tttacaagaa gagtcagatg aagaattaag agaattagct aaaatggagt tagctgagat
    48601 ggaagaaaaa gtagcacctc tggaggaaga aataaaaata ctattattgc ctaaagaccc
    48661 taatgacgat aagaacgtta tagttgaaat tagaggtgga gctggaggag atgaagcagc
    48721 tctatttgcg ggtgatttat ttagaatgta ctcaagatat gctgaaagaa gaagatggaa
    48781 aatagaacta ttgagtgcaa gtgatactgg agttggtgga tataaagaag tatcttttat
    48841 gataaaaggt aaaggtgctt attcaagatt aaagtatgaa tctggagttc atagagttca
    48901 aagaatacca tcaactgaat ctggtggaag aatacacaca tcaacttcta ctgttgcagt
    48961 attaccagaa gttgaagatg tagaggttga aataaatcca aatgatttaa gaatagacgt
    49021 tttccgttct tcaggtaatg gaggacaaag tgtcaatact actgactctg ctgttagggt
    49081 aacacatata cctacaggtg aagtagtatc ttgtcaagat ggaaaatcac aattaaaaaa
    49141 taaagagcaa gctttaaaaa tattaaaagc tagactttac gataaagcat tggcagaaca
    49201 acataaagat atagcagcag aaagaaaaag ccaagtcgga acaggagaca ggtcagaaag
    49261 aataagaaca tataatttcc ctcaaggtag aataagtgac catagaataa atttaacttt
    49321 atataagcta gatgcattct tagatggaga tatagatgaa atgatagatg ctttaattac
    49381 tgttgaccaa acagaaaaaa tgactgcaat ataagtaatg ttttaaagaa gctgtttctt
    49441 tctagaaatt attttctatt ttgaaacagc tattttgcat tttaggtcat gttatcatat
    49501 tctactgtat ataattgtta tatacaagta taggagggtt atatgatact aaaggtaaca
    49561 ataattggac ttttggcagg tgttataggg acaggattag gaggagtaat atcagctatt
    49621 tttaaaagag aggtcgataa atatctaagt ttttttatgg gattatctgg tggaataatg
    49681 ttggctgtag tggtgtttga tttgatgaaa gaatccatgg ataaaatggg aataatcaat
    49741 acagttatat ttacatttgt tggagctctc ataactatgt acataaaaac aaaattagat
    49801 gtgtctggaa atatggcatc tggatatctt atattcataa gtatattact acataattta
    49861 ccagaaggac tagccattgg ttcttctttc atgtcaactg aaagtttagg tattacactt
    49921 gctatagtta taggtctaca taatatacca gaagggttag ctatggcatt aggcttagtt
    49981 tgcaataaaa tgaaactaag taaagttatt ttgtttactg taatagctgg attacctatg
    50041 ggtctaggaa gttttttagg tgtttatttt ggaggagtat ttacttctct tataggagta
    50101 tttcttgcta cagcaggagg tactatgatg tatgttgtat tggaagaaat ttttccacat
    50161 tcaaaaagtg tgtattgtat aattggtttc ttgttaggta caatgattgt aaattatata
    50221 taaaattaaa atgaaagcag gcatacaaaa gatgaaaact attataagta atattgatat
    50281 aaataatatt gattatgagg aaataaaaat acaagcaaaa cttcttagag aaggtaaaac
    50341 agttattttt ccaactgaga cagtatatgg gttgggtgca aatgcattag atgaaaatgc
    50401 agtaaagaag atttatgaag ctaaaggaag acctagtgac aatccattga tagtccatat
    50461 atatgaaaaa aaagaagtat atgacttagc caaaaatata agtgataaag ctaaacttgt
    50521 tatagaaaag ctttggccag gacctataac aattatatta aataaaaaag atataattcc
    50581 atacaaaact agtggagggc ttgatacagt tgcgataagg atgccttcta atattattgc
    50641 cagggcaata ataaaagaag ctggtatacc tattgcagct ccttctgcaa atatctcagg
    50701 tcgcccatct ccaacaaaag caaaacatgt atatgaagag atgaatggaa gagtagatgg
    50761 aatagtttta ggaggagaca gtaattttgg attggagtca actgtattgg atttaacaga
    50821 agaaactcct atgatactga gaccaggaag tataactaaa gaagttttag aggaacttct
    50881 tggagaagta aagcttgacc catcattaag taaaaaagaa gataatcaaa aagctaaagc
    50941 tcctggaatg aaatataaac attattctcc aaatgcagat gtatatataa tatctggaaa
    51001 aagagaaaat gtagcaacca agattaatga tatgataaaa tctaatagag acaaaggtct
    51061 aaagacaggt gtaatgtgta ttagtagaaa ccgagagttt tatgatggag aagtaatcga
    51121 tttaggaaac tctcttgaag aagttgcaag taatctgttt gatgctctta tagaaatgga
    51181 caaaaagggt gttgatgtaa tatattcaga agagttccct aagactggag ttggacaagc
    51241 aattatgaat agactactga agtcagcagg atacaaagta ataaaggctt aagagggagt
    51301 tgtaaaatat gaatatactt attgtgtgta caggtaatac ttgtagaagc cctatggctg
    51361 aggctatact aaggaaagca attaaagaat ctggaagaag tattgaagaa tattctatat
    51421 catcagcagg tatatctaca gcaaatggaa tgagtgcttc tgaaaattct atagaagcac
    51481 taaaggaaat cggtatagac ttatcaaatc ataaaagtaa agtaattact aaaaaattaa
    51541 ttgatgaatc agatataata ctaacaatga ctaaatctca taaagaaatt ttagtacaag
    51601 cagttccaaa atgcaaagaa aaagtgtata cttttaaagg gtttgcaaat aaaaatgagg
    51661 aagatatatc tgacccattt ggtggaaatt tggatattta tagaaacact atgagagaaa
    51721 taatgtattc tgtcaacgaa atagtaaaaa aaatatgaaa cggaggataa acaaatgaag
    51781 ataggattag gctgtgacca tggaggatat aatttaaaaa aagaaataat aagctattta
    51841 gaaggtaaag gtatagaatg tgtagattat ggaactaata atgcgacaga ttctgttgat
    51901 tatcctgttt atggagaaat agttgcaaat tcagttataa ataaagaagt agattatgga
    51961 atattatgtt gtggtacagg aataggaata tctttagctg ctaataaggt ccctgggata
    52021 agatgtgcag ttgtatcaga tgtattttct gcaaaaatgt caaaagcaca caatgatgca
    52081 aatatgttat cattaggcga aagagtttta ggaaaaggtc tagcacttga gatagttgaa
    52141 gcatggatta atacagattt tgaaggagat agacatgcta gaagagtaaa catgattaaa
    52201 tcaatagaag aaaaacataa taaataataa aaattacagt tttatcagga ggaattatca
    52261 aaatgagcaa agtggtagag acaaaccatc cattaataca gcataaatta actttaatga
    52321 gagataaaaa tactggttct aaagacttca gagagctttt aactgaaatt gcaatgttaa
    52381 tggggtatga aataacaaaa gatataccat taaaagatgt agaaatagaa actcctattc
    52441 aaaaaacatc atctaaagta gttgcaggta aaaaattagc aataattcca attttaagag
    52501 caggtcttgg aatggtagat gggttagtaa gcttaatgcc agcagcgaaa gttggacatg
    52561 taggactata tagagaccca gaaacattaa aaccagtaga atattattgt aagcttcctc
    52621 aagatatagg agaaagagat ataatagtag ttgaccctat gcttgcaaca ggaggttctg
    52681 ctgtagcagc tatagattta cttaagtcta aaggagctaa aagtataaaa ttggctaatt
    52741 tagtagctgc accagaaggt atagaagaag tacaaaaata tcatgatgat gttgatatat
    52801 atgttgcatc agttgacgaa agattaaatg aacatggata tataattcct ggtcttggtg
    52861 atgctggaga tagattgttt ggaactaaat aaaaataaat aactataaat atttattagg
    52921 gactttctta tatagtgtat ataattttat gtaagaaggt tcttttttat atattttctc
    52981 aaaataggaa ttattgaatt gataaatttc tcgaattgag aataaaaagt ttttaccaat
    53041 taaaaaagat tgcttatttt acaggttttt atagaatagt agtctatttt aatatggcat
    53101 gttagttgct tacgattaag tttgtaggta ttaagtgtat tagttaaatt gagaatagaa
    53161 tattaataat attattggga gtggtaatac atgaagaatt gtatagtagt aagaggggct
    53221 ggagatttag caacagcagt tattcataaa ttacacaaca gtggttttaa agtagttgcg
    53281 ttagaaatag aagagccatt agctataaga aggcaaattt ctttttgcga agctgtttat
    53341 gaaaaagaag taactgttga aggtgtagaa tgcatatttt gcaatactga aaaagaaata
    53401 gaagaagttt tatctcaaca taaagtagcc ttgattatag acccaaaagg agaaaaatta
    53461 tctaagttga atccaatcgt tttggttgat ggaatacttg ctaagaaaaa cttaggtaca
    53521 actaaagata tggcaccact tacaatagct ttaggtcctg gattttgtgc aggagaagat
    53581 gtagatgttg ttatagagac aatgagaggt cataatctag gaagaataat aactaatggt
    53641 agagcagaaa agaatacagg agtaccagga gcaataaaag gtttttctaa agagagagta
    53701 atgcattcgc agtatgatgg tataatggaa aatgtatgta agataggcga tatagttgaa
    53761 aagggagacc tactttgcta tattaataat tatgaaaaaa aacatgaagt tagagcaaca
    53821 atatctggag tccttagagg tttattaaag gatggagcaa aggtaaacaa aaatttaaaa
    53881 atactagaca tagaccctag aaaagaagaa gttaagaatg cttttacaat atcagataaa
    53941 gctagatgta tagctggagg ggttttagaa gccatactaa ataatgggat ttatccccaa
    54001 aattaattta tattagataa agctattaat attttattaa gatgatataa taaatttaat
    54061 agattaaaga gagcatatga cgggtattta aaatttaaat ggtaaaaatg ctcttttttt
    54121 attggacatt caattaacat gtgtatataa tataaaaata agtatttatt taggttgaaa
    54181 tctaataaat atataatacc agtacataag caaagaggag aaaagtaaat tatgagacca
    54241 tcatgggatg aatacttcat ggaaattgca gaagtagtga aaaagcgttc aacttgtatt
    54301 agaagacaag taggagctgt aattgtaaga gacaaacaaa tattaacaac tggatataat
    54361 ggttctccga gaaatcttga acactgtgaa aatattggat gtaagaggca ggaattaaat
    54421 attccctcag gagaaagaca tgaattatgc agagcattac atgctgaaca aaatgcgata
    54481 atacaagctg ctcataatgg tattagtgta gatggggcaa ctttgtatgt tactacaaga
    54541 ccttgtgttt tgtgtgcaaa aatgtgcata aatgcaggta tattgaaaat tgtttatgaa
    54601 ggtgattatc cagatgacat gtctacagaa ttattaaaag aagcaggaat agaactcata
    54661 aaattttagt ggataaaatt tatatgacaa taaatgtcaa attatgacac tgaaatacaa
    54721 tggtatcaaa tgattaaaag aaagtatttg aaaaatcatc agattagtag taagatatat
    54781 tttataatta aataaattta tgagatagat taattacatg ttatttattt tataataatg
    54841 agggatacta tttatgagta aaaaaaatac acatacagaa attgcaaagt tgtttagttt
    54901 aataagtcaa ataggattga tgattgtaat atcaatatta ggatgtactt tcttaggaaa
    54961 atttttagat agtaaattga atactactcc agttctgaca ataatatttt tattgttggg
    55021 tgttggaggt gcttttagtg gtgtttataa aactctaata gtatatacac gaaggaagtg
    55081 atatattgga tataaaatta cttaaccagg taaggtcagt aacaaaaggt atagtaatat
    55141 ttgatgtagt tttaattgtg ctaatgttga taacatcaac attaagtaaa ccagtattat
    55201 taggacttat atttggctct ataatagcta tgttaaattt tagattattg gcgatatcac
    55261 tggaaaaatc agtgacattt cctgctggaa aggcacaagc ttatgcatca gcacaatatg
    55321 ctattagatt atttataatg gcagttgttc taattgcatc agctacggta ccacatttaa
    55381 atataatcgg aacagcattg ggattattga gcacaaagtt tgtcatatta tcaaaaaact
    55441 ttataaataa attaaagagg aaggaggcat aagtatgaat caagcgcatt attatctcat
    55501 aggaaacttt aagattagtg aaacaatact agtaagttgg ggaataatgg cctttttagc
    55561 gatagcatct tattttttga ctagaaactt aaaaaaagtt ccaactagta aagttcaaat
    55621 attcttagaa tttgcagtag gtggtcttgc taaaatggtt acagatgtta tgggtaaaaa
    55681 tacagtaaaa agaatgccat atatgatacc atacatagga agcttgtttt tatttttcgc
    55741 atgttcaaat ttaagcccat tacttggatt aaggtcacca acaaccgact tagacacaac
    55801 tgctgcatgg gcaatgatta cattttttat gatttatttt gcaggagtta aatttaaggg
    55861 gttatcatac tttaaagaat tggctcaacc acttgccata atgttaccaa tcaatattat
    55921 aggtgagtta gctagaccaa tatcattaag tttccgtcca tttgggaata ttttaggtgg
    55981 aacaataata atgggtctgt tttatcaact tttaggtttt atatctagtt taataccagg
    56041 attatcaata ccaattggac agatattaat accagtccca ttacacttgt actttgactt
    56101 atttgcagga ttgttacaag catatatatt tataatgtta acaatggtat ttatatcaag
    56161 tgcaggagag ttagattaag aaatcaactt atattaagat aaaaataaaa taatttaaaa
    56221 aattaaagtt ttaaggagga aataattatg gaaacagcaa tagtagcggc agcatcagca
    56281 ataggagcag ggatagcagt tgcaacaggt ataggtgcag gtataggtca aggtatagca
    56341 gcagctaaag ctgcagaagc agtaggaaac caaccagaag ctaaaggtga tataacatca
    56401 actttattac taggagttgc tatagcggag tcttcagcaa tatatggttt ggttatatca
    56461 ataatacttc tattcgtaaa tccattcttt aaatacttag gaatgtagat ttagtcctta
    56521 aatattttga gataggttta taggtaaatc atctcaaact tataatcatg agatgaattt
    56581 tatcatctcg tgattataag tagttatgat ttatggaaag gaggataatt atggagaaag
    56641 cactagtagg tataacgtgg gaatttgtgt tccaaattgt taatactttt ataatatttt
    56701 tacttctgag aaagctttta ttcaagccag tattaaatat aattgaatca agagaaaatg
    56761 atattaaaag tgatttggca gaaggcgaaa aagcaaaaaa tgaaggttta gctctgaaaa
    56821 aagaatatga gtcaaaaatc aattttgcca aggatgaagg tcaagaaatc ataaaacaag
    56881 caactataag agcagagcag aaatcagatg atatagtaaa tactgcaaag aaagatgctc
    56941 ttgatataaa agaaaaagca aacaaagata tagaacaaga aagacaaaag gttataaatg
    57001 aaatcaaaaa tgatatatct aacatagcac tgcttgcagc atctaaagtt attgaaaaag
    57061 atttagataa atctaaacat gaagaattga tagaaaactt tataaaagag gtaggggaag
    57121 ctaaatgata aatgtaatag caaatagata tgccgaagcc ctatttcaat taggagaaga
    57181 agaaaattca actgatgtct tattcaaaga actagaaaaa gtagtagata tgatgactaa
    57241 agttagtaaa gatttctaca aagttttaaa atcaccatta gtttcaaaat cagaaaagaa
    57301 aaacttggta gaaattattt ttagtaagga agtaagttct aacataaaaa actttttaaa
    57361 agttttagta gataaagata gaatatcata tttagaagat atcgaattag cttataaaga
    57421 attattaaat aagaaaaaca atgtaattga tggagtagca ataagtgcta ttccaatgag
    57481 tgaaacagat attaaagaac ttgaagtcaa actttcaaat aaatacaaca aaaatgttac
    57541 tatagaaaat gttgttgaca agactatact agggggagtt ctagttagaa ttggaaatga
    57601 acaaatagat ggcactgtga aaactcgtct tgataagatg aaagaaaagc tatctgaagt
    57661 aatatcttaa gaatggcggt gaacccatga acttaaaacc tgaagaaata agttctataa
    57721 ttaaacagca aataaaaaat tatgagaata aagttgagtt gacagataca ggtagtgttt
    57781 taacagttgg ggatggtata gcgagtgtat atggattaga aaaagctatg tctggtgagt
    57841 tgctagagtt cccaggtgaa atatatggta tggcacttaa cttggaagaa gaagtagttg
    57901 gagctgttat ccttggagat gacagtgaaa taaaagaagg cgatatagtt aagagaactg
    57961 gaagaatagt tgaagttcca gttggagaag ctttaatagg tagagttgta aactcactag
    58021 gtcagccaat agatggaaaa ggacctatag cgtacacaaa aactagacca gttgaatctg
    58081 aggcacctgg tataatagat agaaggtctg tttatgaacc attacaaaca ggaatcaaat
    58141 caatagattc aatgatacca attggtagag gtcaaagaga gttaataata ggtgacagac
    58201 aaactggtaa aacatctata gttattgaca ctatactaaa tcaaaaggga aaagatgtta
    58261 tttgtatata tgtagcaatt gggcaaaaac gttctactat agctcaatta gttagctctt
    58321 tagaaaaagg tggagctttg gattatacaa tagtagtttc agcaacagca tctgaatcag
    58381 caccacttca atatatagca ccatatgctg gagcagctat gggtgaagaa tttatgtaca
    58441 atggaaaaca tgtacttata gtgtatgatg atttaagtaa acaagctgtt gcatatagag
    58501 aaatgtcatt gttacttaga agaccaccag gacgtgaggc atatcctgga gatgtattct
    58561 atttacattc aagattactt gaaagagcag ctaagttatc tgatgaattg ggtggaggtt
    58621 caatgactgc cttgcctata atagaaacac aagctggtga cgtttctgca tatataccaa
    58681 caaatgttat atctataaca gatggtcaaa tatacttaca accagagtta ttctattcag
    58741 gtgtaagacc agcagttgac cctggtatat cagtatcaag ggttggtggt tctgcgcaaa
    58801 ttaaagcaat gaaaaaagtt gcaggaacat taaaacttgc atattcacaa tatagagaac
    58861 ttgcagcatt ctcacaattt ggttctgact tagatgaaga tactaaaaag agacttgctc
    58921 aaggtgaaag aatcgttgag atattaaaac aaggtgagca tcaaccaata aaagttgaaa
    58981 atcaagttat gataatatat gctgttataa ataatcatct agaagatatt ccgatagata
    59041 atatagctag atttgaatca gaattgtatg catttgtaga taataattat ccagaaatat
    59101 caagaaaaat attaggtgga gaagacttca ctcatgattt aacagatgca ataaatgaat
    59161 ttaaagaaaa gtttgttgtt gaagtataat cctttttaaa aaaggaggta aattaattgg
    59221 caggagctgg aatcaaagca atcaaaacac gtatgggaac tgttgaaagt acaaagcaaa
    59281 taactaaagc tatggaatta gttgcatctt ctaaacttag aaaagcaaag gaaaaggcag
    59341 aagattcaag agaatatttt aatactttat acaatacagt aaaagatatt gcccaaacaa
    59401 cacgtggaat tagaaatgaa tttttaaaag aaagagaagt aaaaaataag tgctatgtag
    59461 tagtagctgg agacagagga cttgcaggcg gatacaattc caatgtatta aaggaatctg
    59521 tctctcatat gaaaggtaaa aaggagacag tgattactgt tggaaagaaa gcgcatgaat
    59581 ttttttctaa aagaaactac aatatagtaa aagatataaa tagtgttgaa gcttgtggat
    59641 atgaagatgc gacagaaatt tcacaaacta ttattgacct gtataaaaaa ggtgaagtag
    59701 atgaggttta tattgcttat acaaaattta aatctgcttt atcacaagaa gttgaaattt
    59761 tgaaattgct tccattagtc tttgaaaaag atacagacga acaaggaatg gcagtaaaag
    59821 aagaaaaaca aaatagatca aaagtacaat atataccatc agcagaagca gttttaagtt
    59881 atattgtacc tcagtttata tcaggtacag tatatggtgg aattttggaa tcttttgcat
    59941 cagagcaagg tgctagaaga actgcgatgg aatcagccac agacaatgcg aatgaaatgc
    60001 tatctaattt agagttaagt tataacagag caagacagtc agctgtaact caagaaataa
    60061 cagagatagt tggtggagtt gaagctctaa aatagggagg ttaggaaatg gcaaatgtag
    60121 gtaaagtagt acagattgta ggagctgtac tagatgttaa gtttgatagt gaacaaagcc
    60181 tacctaactt attaaatgcg ttagtaataa agttaggaga taaagaaata gttgcagaag
    60241 ttgctcaaca tataggtgat gatactgtaa gatgtatagc tatgagtgca acagacggac
    60301 ttgtaagagg tatggaagtt gtagatacag gaggaccaat aagtgtccct gttggagatg
    60361 agactttagg gagaatattc aatgttcttg gaaaaccagt tgatgggaaa ccagctccaa
    60421 aatctgcacc taaattacct atacatagac cagcaccggc atatgatgaa ctagaaacta
    60481 cagcagagat actagaaact ggtattaagg tagttgactt actagctcca tatctaaaag
    60541 gtggaaaaat aggtctattt ggaggagcag gagtaggaaa gacagttcta atacaagagc
    60601 ttataaacaa tattgctaag caacatggtg gtatttctgt attttcaggc gtaggagaaa
    60661 gaacaagaga aggtaacgac ctttatggcg aaatgagtga gtctggagtt ataaataaaa
    60721 cagctctagt atttggtcaa atgaatgaac cacctggagc gagaatgaga gttgctttaa
    60781 ctggacttac aatggcagag cattttagag atgagcaagg acaagacgtt ttacttttcg
    60841 ttgataatat attccgtttc acacaagctg gttcagaagt ttcagcactt ctaggacgta
    60901 tgccatcagc tgttggttat cagccaacat tagctactga aatgggtgca cttcaagaga
    60961 gaataacatc aactaagaaa ggttcaataa catctgttca agcagtatat gtaccagcag
    61021 atgacttaac tgacccagca ccagctacaa cattctctca cttagatgca aaaacagttt
    61081 tatcaagaca aatatcatca ctaggtatat atccagcagt tgaccctctt gagtctacat
    61141 caaggattct tgaccctagt atagtaggaa aagaacatta tgaagtagct agaggtgtac
    61201 aatcaatact tcaaagatat aaagaacttc aagatataat tgctatactt ggtatggatg
    61261 aactatctga tgaagataag ttaatagttg cacgtgctag aaagatacaa agattcttat
    61321 ctcaatcatt cacagttgct gagcaattta caggaaatcc aggtcaatat gttcctgtta
    61381 aagagactgt aagaggattt aaagaaattc ttgaagggaa acatgatgac ttaccagagt
    61441 cagcattctt atttgtagga acaatagaag acgctgttag aaaagcgaag ggaagtatgt
    61501 agtcatggcc agtgaatttt cattgaaagt tgtaactcca agtaatactt tttatgatgg
    61561 agatgttgaa atgatagtag ttagaactac aaaaggagac aaaggggtgt tgaaaggtca
    61621 tataccattt gtttcaggta tacctgtagg aactttaaag ataaaaaaag agggaagttt
    61681 taaagaggcg aaaatagcag aaggattcat caatgtaact caagataaaa ctgtcatatt
    61741 gacagaatct gctgagtgga tttaataatt taatttagaa tataaaaagt cattttgacc
    61801 aaaaaaactt ttgggtaaaa tggctttttt gtattttgat gttaataaaa taacgataaa
    61861 attgtaaatt tttagtgata ataaattcac aaaatgttta aatttactgc aaaattatgt
    61921 tgtaattaat aagataaaga agatttagat agcttttatg taagtgaaaa agtcttccta
    61981 taagaaatac atataaaatt tttataaaat ttaaaaaatt tgct
//
LOCUS       DAEMAE010000002        61691 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEMAE010000002 DAEMAE010000000
VERSION     DAEMAE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.002"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..1280
                     /locus_tag="KPH93_000062"
     CDS             84..1280
                     /locus_tag="KPH93_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG1686910.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1373..1897
                     /locus_tag="KPH93_000063"
     CDS             1373..1897
                     /locus_tag="KPH93_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG1686911.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1922..2098
                     /gene="rpmF"
                     /locus_tag="KPH93_000064"
     CDS             1922..2098
                     /gene="rpmF"
                     /locus_tag="KPH93_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG1686912.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2305..2862
                     /gene="fapR"
                     /locus_tag="KPH93_000065"
     CDS             2305..2862
                     /gene="fapR"
                     /locus_tag="KPH93_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG1686913.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2873..3895
                     /gene="plsX"
                     /locus_tag="KPH93_000066"
     CDS             2873..3895
                     /gene="plsX"
                     /locus_tag="KPH93_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG1686914.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3921..4907
                     /locus_tag="KPH93_000067"
     CDS             3921..4907
                     /locus_tag="KPH93_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG1686915.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVSEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5016..5945
                     /gene="fabK"
                     /locus_tag="KPH93_000068"
     CDS             5016..5945
                     /gene="fabK"
                     /locus_tag="KPH93_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG1686916.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5961..6911
                     /gene="fabD"
                     /locus_tag="KPH93_000069"
     CDS             5961..6911
                     /gene="fabD"
                     /locus_tag="KPH93_000069"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG1686917.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     NAIDFKDAVCLVKKRGKFMQETVPTGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6911..7660
                     /gene="fabG"
                     /locus_tag="KPH93_000070"
     CDS             6911..7660
                     /gene="fabG"
                     /locus_tag="KPH93_000070"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG1686918.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7714..7938
                     /gene="acpP"
                     /locus_tag="KPH93_000071"
     CDS             7714..7938
                     /gene="acpP"
                     /locus_tag="KPH93_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG1686919.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7967..9205
                     /gene="fabF"
                     /locus_tag="KPH93_000072"
     CDS             7967..9205
                     /gene="fabF"
                     /locus_tag="KPH93_000072"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG1686920.1"
                     /translation="MNKRVVITGLGCVTPLGTGKDEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKTDRERVGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9431..11149
                     /locus_tag="KPH93_000073"
     CDS             9431..11149
                     /locus_tag="KPH93_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1686921.1"
                     /translation="MNFRLPKKRLPKKIIFISIILISIILFIKYDQNGLREREIINRG
                     KYLSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASILLKVGSYETAEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEVGEY
                     DKAIEISSRIKDYKKFVNTDDYNDFEIMASIMQSNAYIGKNNIQKAKTFLEKADYLIK
                     SDRTVHILDKDMYYYMAMGNLEYKSKNYNSAIDNYKKSLEISTKRKLIQEKIKNLNKI
                     LNIYEKQGNVKSLNEYQKILINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYIEKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDVVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVVDIIDNIRVAIANKKWSEDVTVTISAGVAHISGSKNVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11180..13180
                     /locus_tag="KPH93_000074"
     CDS             11180..13180
                     /locus_tag="KPH93_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1686922.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKED
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDVISEDLNILLGDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCIELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLPKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIKVECANNYEDGFGYTRKEFEEILKILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13532..13777
                     /locus_tag="KPH93_000075"
     CDS             13532..13777
                     /locus_tag="KPH93_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG1686923.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13812..14537
                     /locus_tag="KPH93_000076"
     CDS             13812..14537
                     /locus_tag="KPH93_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG1686924.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDVNPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCNKMEKLFKYFINICSNL"
     gene            14559..15872
                     /locus_tag="KPH93_000077"
     CDS             14559..15872
                     /locus_tag="KPH93_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1686925.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKAFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            16078..16617
                     /locus_tag="KPH93_000078"
     CDS             16078..16617
                     /locus_tag="KPH93_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1686926.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16706..18691
                     /locus_tag="KPH93_000079"
     CDS             16706..18691
                     /locus_tag="KPH93_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBG1686927.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTIILKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18912..19880
                     /gene="spoIIIAA"
                     /locus_tag="KPH93_000080"
     CDS             18912..19880
                     /gene="spoIIIAA"
                     /locus_tag="KPH93_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG1686928.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKISNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGIPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19870..20391
                     /locus_tag="KPH93_000081"
     CDS             19870..20391
                     /locus_tag="KPH93_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG1686929.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20408..20605
                     /gene="spoIIIAC"
                     /locus_tag="KPH93_000082"
     CDS             20408..20605
                     /gene="spoIIIAC"
                     /locus_tag="KPH93_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG1686930.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20621..21007
                     /gene="spoIIIAD"
                     /locus_tag="KPH93_000083"
     CDS             20621..21007
                     /gene="spoIIIAD"
                     /locus_tag="KPH93_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG1686931.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21092..22297
                     /gene="spoIIIAE"
                     /locus_tag="KPH93_000084"
     CDS             21092..22297
                     /gene="spoIIIAE"
                     /locus_tag="KPH93_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG1686932.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLVGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22357..22974
                     /locus_tag="KPH93_000085"
     CDS             22357..22974
                     /locus_tag="KPH93_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG1686933.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGSYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNSSSNEENKNNSTKREEKNIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22986..23540
                     /locus_tag="KPH93_000086"
     CDS             22986..23540
                     /locus_tag="KPH93_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG1686934.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23567..24256
                     /locus_tag="KPH93_000087"
     CDS             23567..24256
                     /locus_tag="KPH93_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG1686935.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDGDSSKTEDSSNVDKQDSKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKT
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24366..24728
                     /locus_tag="KPH93_000088"
     CDS             24366..24728
                     /locus_tag="KPH93_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG1686936.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24813..25319
                     /gene="nusB"
                     /locus_tag="KPH93_000089"
     CDS             24813..25319
                     /gene="nusB"
                     /locus_tag="KPH93_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG1686937.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25303..26373
                     /locus_tag="KPH93_000090"
     CDS             25303..26373
                     /locus_tag="KPH93_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG1686938.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVKDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNRFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26370..27575
                     /locus_tag="KPH93_000091"
     CDS             26370..27575
                     /locus_tag="KPH93_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG1686939.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKVIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27577..27792
                     /gene="xseB"
                     /locus_tag="KPH93_000092"
     CDS             27577..27792
                     /gene="xseB"
                     /locus_tag="KPH93_000092"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG1686940.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27795..28682
                     /locus_tag="KPH93_000093"
     CDS             27795..28682
                     /locus_tag="KPH93_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG1686941.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANFEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28712..29149
                     /locus_tag="KPH93_000094"
     CDS             28712..29149
                     /locus_tag="KPH93_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG1686942.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29183..31048
                     /locus_tag="KPH93_000095"
     CDS             29183..31048
                     /locus_tag="KPH93_000095"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG1686943.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEDMLKVLLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNADELYDFVGLSPKSIADKIRKLVIE"
     gene            31181..31993
                     /locus_tag="KPH93_000096"
     CDS             31181..31993
                     /locus_tag="KPH93_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBG1686944.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEIVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGEIYNNKIVEVVEASHNLDK"
     gene            32013..33692
                     /gene="recN"
                     /locus_tag="KPH93_000097"
     CDS             32013..33692
                     /gene="recN"
                     /locus_tag="KPH93_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG1686945.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNIYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEGFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGLFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33908..34774
                     /locus_tag="KPH93_000098"
     CDS             33908..34774
                     /locus_tag="KPH93_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686946.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDIGNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLMYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34867..35358)
                     /locus_tag="KPH93_000099"
     CDS             complement(34867..35358)
                     /locus_tag="KPH93_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1686947.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEYKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35419..35859)
                     /locus_tag="KPH93_000100"
     CDS             complement(35419..35859)
                     /locus_tag="KPH93_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1686948.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WIPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36020..37078
                     /locus_tag="KPH93_000101"
     CDS             36020..37078
                     /locus_tag="KPH93_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG1686949.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDIDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKNKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNIIENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37220..38044
                     /gene="spo0A"
                     /locus_tag="KPH93_000102"
     CDS             37220..38044
                     /gene="spo0A"
                     /locus_tag="KPH93_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG1686950.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38457..39578
                     /locus_tag="KPH93_000103"
     CDS             38457..39578
                     /locus_tag="KPH93_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686951.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39596..40471
                     /locus_tag="KPH93_000104"
     CDS             39596..40471
                     /locus_tag="KPH93_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG1686952.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKNIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDNTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40495..41187
                     /locus_tag="KPH93_000105"
     CDS             40495..41187
                     /locus_tag="KPH93_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1686953.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSKLENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41195..42019
                     /locus_tag="KPH93_000106"
     CDS             41195..42019
                     /locus_tag="KPH93_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG1686954.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYTFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42024..43091
                     /locus_tag="KPH93_000107"
     CDS             42024..43091
                     /locus_tag="KPH93_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG1686955.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDILKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43093..43629
                     /locus_tag="KPH93_000108"
     CDS             43093..43629
                     /locus_tag="KPH93_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1686956.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43697..44302
                     /locus_tag="KPH93_000109"
     CDS             43697..44302
                     /locus_tag="KPH93_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG1686957.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAVFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44314..45228
                     /locus_tag="KPH93_000110"
     CDS             44314..45228
                     /locus_tag="KPH93_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1686958.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45270..46442
                     /locus_tag="KPH93_000111"
     CDS             45270..46442
                     /locus_tag="KPH93_000111"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG1686959.1"
                     /translation="MSRVIWIVIDSVGIGALPDAENFGDSKDVSTLGNIFKKHPDIQI
                     PNMRKLGIGNIDGVDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EDGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIETGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMRKDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46489..47301
                     /locus_tag="KPH93_000112"
     CDS             46489..47301
                     /locus_tag="KPH93_000112"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG1686960.1"
                     /translation="MFEKIAQSSEFINSKINVKPKIGLILGSGLGDLANDIEDSVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47505..48830
                     /locus_tag="KPH93_000113"
     CDS             47505..48830
                     /locus_tag="KPH93_000113"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG1686961.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVYLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSAEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLIASSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKRRFIDSYSIVDSKIEKNKLIYGIVTKEDIKKF"
     gene            49043..50122
                     /gene="mltG"
                     /locus_tag="KPH93_000114"
     CDS             49043..50122
                     /gene="mltG"
                     /locus_tag="KPH93_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG1686962.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIESIEAALYPDKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKR"
     gene            50294..50968
                     /locus_tag="KPH93_000115"
     CDS             50294..50968
                     /locus_tag="KPH93_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG1686963.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKERKRG
                     DVDGIK"
     gene            50955..52202
                     /locus_tag="KPH93_000116"
     CDS             50955..52202
                     /locus_tag="KPH93_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG1686964.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEDIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52274..53938
                     /locus_tag="KPH93_000117"
     CDS             52274..53938
                     /locus_tag="KPH93_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG1686965.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSALIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLVVEKEKLNNSYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEVSTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNDMNT
                     IKTYKSSKKEDLISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54035..54319)
                     /locus_tag="KPH93_000118"
     CDS             complement(54035..54319)
                     /locus_tag="KPH93_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1686966.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54342..55859)
                     /locus_tag="KPH93_000119"
     CDS             complement(54342..55859)
                     /locus_tag="KPH93_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1686967.1"
                     /translation="MSVAIYLRKSRADEDAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKSSDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEEQ"
     gene            complement(55984..56805)
                     /locus_tag="KPH93_000120"
     CDS             complement(55984..56805)
                     /locus_tag="KPH93_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1686968.1"
                     /translation="MIISKKITAILVSSLIMISMVGCVPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTDL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDNDYNKAAIDIKKAIEIDDKNPPAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            56996..58423
                     /locus_tag="KPH93_000121"
     CDS             56996..58423
                     /locus_tag="KPH93_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG1686969.1"
                     /translation="MKIKKLLTIGLSLSMFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            complement(59752..59976)
                     /locus_tag="KPH93_000122"
     CDS             complement(59752..59976)
                     /locus_tag="KPH93_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1686970.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSIMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            60666..60821
                     /locus_tag="KPH93_000123"
     CDS             60666..60821
                     /locus_tag="KPH93_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG1686971.1"
                     /translation="MTLEVKIKVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            61067..61318
                     /locus_tag="KPH93_000124"
     CDS             61067..61318
                     /locus_tag="KPH93_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686972.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
BASE COUNT        24764 a         6374 c        11194 g        19359 t
ORIGIN      
        1 aaggtagagt tttatttaga catttattac aaatcaaaat ttattacata ttatttataa
       61 aaaaatctaa ggaggaaatg ataatgaaaa tattagtatt aaactgtggg agttcttcat
      121 taaaatatca attaatagat atgaataatg aagaagtttt atgtatagga ttggttgaaa
      181 gaataggaat agaaggttct atattaaaac atgaaaaagc aggtagagat gataaatacg
      241 ttgttgaaca accaatgaag gaccacaaag atgcaatagc attagtttta gaagccgttg
      301 ctcatccaga atttggtgca gttaaagaaa tgaaagaaat agatgcagta ggacatagag
      361 ttgtacatgc tggagaaaaa tttgcaactt cagtagtaat aactccagaa gtagaagaag
      421 ctttaaaaga atgtatagat ttagctccac ttcataatcc agcaaacata atgggaatag
      481 atgcttgtaa agctatactt ccagatgtac caatggtagg tgtatttgat actgctttcc
      541 atcaaacaat gcctaaatct tcatacttat atggtttacc tcatgagtta tatactaaat
      601 atggggtaag aagatatgga ttccacggaa cttctcataa ttatgtatct caaagagcag
      661 cagagatatt aggaaaagat ataaaagatt taaaaatagt aacttgtcac ttaggaaatg
      721 gtgcttctat agctgctgtt gatggtggaa aatgtgtaga tacatctatg ggattcactc
      781 cattagaagg attaataatg ggaactagat gtggagatat agacccagct atattaccat
      841 tcttaatgag aaaagaaggt ttagatgctg atggattgga taaattaatg aataaagaat
      901 ctggagtata tggaatgact ggaatttcta gtgacttcag agatatagaa gatgcagcta
      961 aaaatggaga cgaaagagct caagctacat tagaagcgta tgttaaaaaa gtacaaaaat
     1021 atataggtgc ttatgctgct gaaatgaatg gattggatgt tgtagtattt actgcaggcg
     1081 ttggtgaaaa tggaaaggct ataagagcag atattgcttc taacatggag tttttaggaa
     1141 tgaaattaga taaagaagct aatgatgtta gaggaaaaga aacagtaata tctactgctg
     1201 actctaaagt aaaaatgctt ttaataccaa ctaatgaaga attaatgata gctagagata
     1261 cattaagatt ggtaaaataa ttaaattaaa cctatgccta tcaagtaaaa atacttgaca
     1321 aacaaatgct agatttgtat aataaatttg gtgatatgtt gaggtgaact taatgaaagt
     1381 tagcatagaa aaaataaata gaaaagaaac tgacaaaata gacttgaatt tttgtgaaaa
     1441 aattgatact attagttatt gtgatgagat ttacaaatta gtatcacctg taaatttaaa
     1501 agggaaagtg tcaaagacta ataaaggttt gtatctagat atagatgtca attttacaat
     1561 tgttgacaat tgttcaagat gtcttaaaga agtagaaata ccactagagt attctataca
     1621 aggtttttta gtaaaagaag aagattatga tgaagatgag tttgaggaat ttgatccttt
     1681 tatattcgat ggtgaagaaa tcgaccttat tgatataata gaacagacat tagattttaa
     1741 tgtacctcat aaagttcttt gtagtgaaaa ctgtaaaggt ctttgtcaag tatgcggggc
     1801 aaacttaaat gaagaagagt gttcttgcag tgaaattaca aacgatgagg aatacataga
     1861 tcctcgtttt gctaaattaa aagatttatt taattaaatt ttgactaagg aggtggcgta
     1921 aatggcagta ccaaagcgta aaacgtctaa atcaaacaca aaaatgagaa gagcagctaa
     1981 ctcaaagatg gaagcaacag gatttgtaag ttgcccacaa tgtcatgagc caaaattacc
     2041 acatagagtg tgtccagact gtggatatta taaaggtaaa gaagttgtgt ctaagtaata
     2101 gcttctaaaa agcatgtaga gtatactcta catgtttttt ttgtacatat aaaaaaataa
     2161 gatgatttac tagagaaaat aagtggattt tatgcatatt aaaaaataga aaaataataa
     2221 tataagtatt gaaaattttt gtgtgttact atatactact attagtagct ggtactaatc
     2281 acagctataa aatttggagg gaacatgaaa aagaaaagta aagcccaaag acaaaaagag
     2341 cttatagata tgctaaagac agatcctttt tatactgatg aagaattatc aagtctgttt
     2401 gatgtaagta tacagaccat aagattagat agaatgagtc taaacatacc agagctaaga
     2461 gaaagagtca aatcaatagc agaaactcag agttcaaaag taaagacttt aggagtaaag
     2521 gaaattacag gagaaatcat tgatttatct gtagggcgat taggtatatc tatgttagaa
     2581 gttacacaag acatgattta ctctaagact aatactttaa aagatacgta tatattttct
     2641 ctagcagatt cattggcaat ggctataata gatgcaccta aggttattat gagagttgca
     2701 aatgtaaaaa gttttaagtt gatagaacaa caagataggc ttatagcaaa agcagaagtg
     2761 tatagaaata tagataaaaa gcattatgta aaagttgtta tcaacaataa agcccaagag
     2821 caaatattta gagggaagtt tatctttgaa gaattagatt aggagggaaa ttatgaaaat
     2881 agtaattgat ggaatgggtg gagataatgc acctaaatct aatgtagaag gcgcagtaaa
     2941 tgctataaaa gaatatcagg ttgatttaat aatcacagga gataaagatt tacttgaaaa
     3001 ggaattttca aattatgaat ttgatagaaa taaattagag atagttcata ctactgagat
     3061 tatagaaaac gaagataaac cagtaaaggc tataagaagt aaaaaagatt catctatggt
     3121 agtagcatta aatcttgtaa aagaaggaaa ggcagatgct ataatatcag ctggaaatac
     3181 aggagcctta cttgctggtg gattatttgt tgttggtaga ataaaaggaa tcgatagacc
     3241 ttgcttatgt tcagcaatac caaatgtaaa gcgaggaatg acacttatag cagactgtgg
     3301 cgcaaatgca gattgtaaac ctaagaatct agttgaattt gcagctatga gtaatattta
     3361 ttcaagaaaa gttttaggtc ttgaaaatcc taaggtagca ttagcaaatg ttggactaga
     3421 agaaggcaaa ggcaatgatt tagtcaaaag gtcatatgaa gaaattaaaa aattagattt
     3481 aaattttata gggaatgtag aagctagaga agttataaat gcatacacag atataatcat
     3541 atgtgatgga tttacaggta atatattact taaatctgct gaaggtgtag cactttcagt
     3601 tatgagtctt ataaaagaaa cttttatggc cagtacaaaa agtaaaatag gagcattact
     3661 tataaaggat gatttaagaa aattaaaaag ttttatagat tattctgagt atgggggagc
     3721 tccactttta ggtctaaatg gaggagttat aaaagctcat ggtagttctg atgctaaagc
     3781 tataaaaaat gctataaatc aaggaataaa attttcaaag ggaaaagttg ttgaagatat
     3841 taaccaattt ataagcaaat acaatgaaga aaataaaaat aacgaagatg aataaactcg
     3901 ttttggaggt ttaatatata atgaatacaa aggctggaat attaggagta ggaagttatc
     3961 tacctgagca atcatatgat aattttcact tcgaaaaaat aatggataca tcagatgagt
     4021 ggataagtac tagaactggt attaaggaaa gaagatttgc caaagagtct gaagcaactt
     4081 ctgacttagc atctaaagct gccttaaaag ctatagaatg tgcaaagtta aacgtagagg
     4141 atatagagct gattatatta gctacaatta ctcctgatat gtctttgcct tcaactgcat
     4201 gtatagtaca agatgcaata ggagcagtga atgctactgc atttgatata tcagcagctt
     4261 gttctggatt tgtatatgga gtaactattg caaagcaatt tgtggaaaca ggttgctata
     4321 aaaatgtgct tgtaatagga gcagaaactt gttctaagtt tttaaattat gatgatagaa
     4381 caacagcagt attgtttgga gatggagctg gagcagctgt tattggtcct gtaagtgaag
     4441 gtggaatact gtctacacat atgggctctg atggtaaagg aaaagattgt ttaaaagtac
     4501 ctgctggagg ttcaagactt aaggcaagta aagagactgt agaagctaat ctacatacaa
     4561 tagaaatggc aggtagtgat gtgtttaaat ttgcagttag aaagatggca gaaacttctt
     4621 taagagctct tgagaaagca aatctaaata cgactgatat agactactta gtgccacatc
     4681 aagctaatat aagaatcata caagcttcct ctaagagatt agagcttgat atgaaaaaag
     4741 tttatgtaaa tatagacaaa tatggaaata tgtctgctgc atctatacca gtagcattag
     4801 atgaagctta tagagaaggt aaaattaaaa agggtgataa tgtagtttta gttggctttg
     4861 gtggaggtct aacttggggg gcttcagttg tcaagtggac tttatagtat aaaatatcta
     4921 aaatgcctta attaacaaaa ttaaacttat acacaaaata aagttgatta ataataaaaa
     4981 gtttactttt atgatttcaa gtgaaggaga attgtatgaa taaaatttgc aaaatattaa
     5041 atataaaata cccagttatc caagggggaa tggcatgggt agctactgca tcattagcaa
     5101 gtgctgtatc taatgcagga gggcttggta taatagcagc aggaaacgca ccaaaagaag
     5161 ctataaagaa agaaattgtt gagtgtaaaa aattaacaga taaacctttt ggagtaaatg
     5221 taatgcttat gtcgccattt gttgatgata taattgattt gattatagaa gaaaaagttc
     5281 aagttattac tactggtgct ggaaatcctg caaagtatat ggatagatta aaggaagctg
     5341 gaacaaaggt tattcctgta gtacctacaa tagctttggc gcaaagaatg gaaaagctag
     5401 gagctacagc agtaatagca gagggtactg aaggtggagg acatatagga gaacttacta
     5461 ctatggtctt agttccacaa gttgctgatg ctgtaaacat acctgtaata gctgctggag
     5521 gaattgtaga tggtagagga attgcggcat catttgcatt aggtgccagt gcagttcaag
     5581 taggcactag atttatttgc agtgaagagt gctctgtcca ttcaaactat aaaaacttag
     5641 tactaaaagc aaaagataga gatgcaattg taacaggaag aagtactggt catccagtaa
     5701 gaacattaaa aaataaatta tcaaaagaat ttttaaagat ggaacaaaat ggagctactc
     5761 ctgaagaact ggataaaaaa ggtacaggag ctttaagatt tgcaacagta gatggagaca
     5821 tagaaaaagg ttcatttatg gcaggtcaaa gtgctgctat ggtaaaagaa ataacacctt
     5881 gtaaggaaat tatagaggct atggtaaatc aagcaagaga gattatgcca gcaatagaac
     5941 tgtaaatcag gaggagtaag atgggaaaag ttgcactagt attccctggt caaggagctc
     6001 aatatgtagg tatggccaag gatttatatg aaaataatgc tgttgctaaa agtgtaatag
     6061 atgaggctag tgatgcctta aacatggatt taaaaaacct tatgtttaat ggaaatgaag
     6121 aagaactttc taaaactgaa aacactcaac ctgctatggt tacacatagt gtagcagtat
     6181 taaaagcagt acaagctcaa atagatttaa aatataatgc atgcttagga ttatccttag
     6241 gagagtatag tgcgttggtt gcagcaaatg caattgactt taaagatgct gtatgtttag
     6301 ttaaaaaaag aggtaagttt atgcaggaaa cagttccaac aggtgttgga gctatggcag
     6361 caattttagg tttagataga aacgtacttg aaaatgtggt taaagatata caaggaggta
     6421 ttgtagaagt agcaaattac aattctcctg gacaaatagt tatatctggt gaaaatgaaa
     6481 agatagaaga agcaatggta aaatgcaaag aagcaggagc aaaaagggca gttaaattaa
     6541 atgtaagtgg accttttcac tcatcaatgt taaaagaagc tggtgtaaaa ttggctagtg
     6601 aattagaaaa agttaacata actaaaccta aagttgatgt ggtggcaaat gtaaatgcag
     6661 attactataa aaacgaaggt aaagacctgc ttataaaaca agtaagttca tctgttcttt
     6721 gggaagattc tatagaaaga cttttaaatg atggatatga tacttttata gaaatgggac
     6781 ctggaaaaac tttaaaagca tttattaaga agatagcttc caataaaaaa gcaaatgtca
     6841 atatttataa tatagatggt atagattctc ttaatgaatt tgtacaaaat tatagaaatg
     6901 gagagatata atgataaatc ttacaggaca agtagcagtt gttactggtg gctctagggg
     6961 tataggaaaa gaaatagcaa aaaaactagc atcttttgga gctgatgtag taatcaatta
     7021 tacttctaaa gaagatgaag cactaaaaac taaaaatgaa atagaaagta tgggggtaaa
     7081 gtgtacctct ataaaatgtg atgtgtctaa atttgatgaa gtgaatcaaa tgatagattc
     7141 tgttgtaagc gaatttggaa aaattgatat attggttaat aatgcaggca taactaaaga
     7201 tggtctgctt atgagaatga aagaagaaga ttttgataga gttatagata taaacttaaa
     7261 aggtgtcttt aattgtacaa aagcagttac caaacctatg atgaaaaaga agtatggaag
     7321 aataataaat atgacttcag tagttggaat tatgggtaat gcagggcaaa ctaattattg
     7381 cgcatcaaaa gcaggtgtaa ttggatttac aaaagcttct gcaagagagt tagcatcaag
     7441 aaacataaat ataaatgcag tagcacctgg atttatagaa acagatatga ctaaagtact
     7501 aagtgatgat gtaaaagagt caacactagc aaatatacca aagaaatctt atggtaaacc
     7561 agaagatgta gccaatgccg tagcattttt agttagtgac atgtcaagtt atataacagg
     7621 acaagtaata aatgtagatg gtggaatggt aatgcaataa taatttaaaa agtagagaaa
     7681 tctattaaaa taaaaaattt taggaggtac acaatgttta ataaagttgt agaaataata
     7741 atagagcaat taggagtaga agataaagaa ataactatgg agacatcatt aatgaaggat
     7801 ttagaagcag attcattaga tgcagtagaa ataataatgg cacttgaaga tgaatttggt
     7861 atagaaatac cagatacaga agcagaaaac ttcaaatcta taggagatat agttaagtat
     7921 atagaagcta ataaatagtc gagctttaaa aaagtggagg tatccgatga ataaaagagt
     7981 agtaataaca gggcttggat gtgttacgcc gttgggaacg ggaaaagatg agttctggag
     8041 caatataaaa tctggagtat ctggtataga taaaataact aatttcgatg ctagcacgta
     8101 tcaaactcag atagcaggag aagttaagaa ttttcatcca gaagaatata taagcaaaaa
     8161 agaattgaag agattagata agtttgctca atttgcaata gtgtctgcaa aattagcagt
     8221 tgaagatgca aatcttgatt tagataaaac agatagagag cgtgttggtg ttataatagg
     8281 ttctggaata ggtggagttg aagctataga aactcaacat aaaattcttt tggaaaaagg
     8341 aaataaaaga gtaagttcac tttttgtgcc tatgatgata ggaaatatgg cagctggtca
     8401 agtttcaata tttttaggag caaaagggcc taatactaat gtatgtacag cttgtgcatc
     8461 aggaactcat tcaataggtg atgcttttaa ggtaattcaa agaggtgatg cagacattat
     8521 ggtagcaggt ggctctgaag ctgctgtaac aggtcttgct tttgctggat tctgtaacat
     8581 gaaggctatg tcaactagaa atgatgaccc taaaactgca tcaagaccat ttgataaaga
     8641 cagagatggt tttgtaatgg gagaaggagc aggtatcgtt atcttagaag acttagaaca
     8701 tgctttagca agaggggcta aaatatatgc agaagtggtt ggatatggtt taactgctga
     8761 tgcataccat atgacaactc cagctgaaaa tggagaggga gcagctaggt ctatgaatat
     8821 ggctttgaaa gatggaaatg ttccattaga agaagtagat tatataaatg ctcatggaac
     8881 atcaacttat tacaatgatt tatatgaaac tatggctata aaaactgtat ttggagaaaa
     8941 ggcatatgac ttatgtgtgt catcaacaaa atctatgact ggtcacttat taggagcatc
     9001 aggtgctata gaggctgttg tttgtgctat gagtatagaa gatagttttg tgcctccaac
     9061 tataaacata caagaagttg gtgaagattt ggatttagat tatgttccaa atcaaggtaa
     9121 agaaaaaaat attagatatg cactatcaaa ttctttagga tttggtgggc ataatgctac
     9181 aatagtacta aaaaaatatg tataaataaa tattaaatat aaaattaaaa aacaagtact
     9241 cgttttaact gtataaattt atattggctt aaatttaaag tctcctaaat tattttagga
     9301 gactttttta ttaagattta agtctagcat atgttgtaaa tcattttgat atattgtatt
     9361 attaattatt aagaggttat ttatatatgc aagttttcat tctacaatat acaaaaggag
     9421 gactaaaatt ttgaatttta gactaccaaa gaaaaggtta cccaaaaaga taatttttat
     9481 aagtataata cttattagta ttattttgtt tataaagtat gaccaaaatg gattgagaga
     9541 aagagaaatt ataaataggg gaaaatatct gtctgaaagt cttgtggcag ataaagagta
     9601 tgatgaagca aaaaaagtgg ttaagacttc ttttgaatct atcccagaaa aaaaatacaa
     9661 taaagtatat aatgaagttt gggatatgtt aaagactatt tcgtatgttc cagatggaac
     9721 gaagatagtc attgattctt tagaaaagat gagagaatca gatgtaaagt tatctgatga
     9781 gtgtagattt aatatagaaa aaagattagc atcagtgtat ataatggata ataattattc
     9841 taaagcagta gatttaactg taaagtctat aaaattggct gagaaattgg ggaataatta
     9901 tgaaaaagca agactagatg ttgatttggc gagcatactt ttaaaggtag gtagctatga
     9961 aacagcagag aaattaataa aagattcttt taagatagat attgacgaag gtgaaaaaaa
    10021 tggaatggtt agattatatg ctattataaa tctagcagaa atatatgtag aggtgggaga
    10081 atatgataaa gccattgaaa taagtagcag gataaaagat tataaaaagt ttgtaaatac
    10141 agacgattac aatgattttg agataatggc ctctataatg cagtcaaatg cgtatatagg
    10201 aaaaaataat atacaaaagg caaagacttt tttagaaaaa gctgattatc ttataaaatc
    10261 agatagaaca gttcatatat tagataaaga tatgtactat tatatggcaa tgggaaactt
    10321 agaatataaa agcaaaaatt ataattctgc aatagataac tataaaaaaa gtttagagat
    10381 atctaccaaa agaaagttaa ttcaggagaa aataaaaaat ttaaataaga tattaaatat
    10441 atatgaaaaa caaggaaatg taaaatcttt aaatgagtat caaaagatat taataaatga
    10501 atataaagag aacaaaagta taagagattc agagactagt ttctatataa ttgataaagt
    10561 atcaaatgaa agtaagctat ttgaaaaaac taaaaaagaa ataaaatatt ataaaatatt
    10621 atttacagta attttaattg ctatattagg gtctactttt ttatacaaca gattgaagta
    10681 ttttaaagca caaaatttac atgatggtct tacaaatgtt tataatagaa aaagttttga
    10741 tattatgtat gaaaagtaca tagagaaaga tgataatttt gcacttgtga tgatagatat
    10801 tgataatttc aagctcataa atgataatta tggacataaa tttggtgacg ttgttataaa
    10861 gggaataaca aacaccattt gtgttatgtt agaacaagat gataaagtct ttaggtacgg
    10921 aggagaagaa ttttctgtat taataagaaa taaatctggt gaggaagtag tagatataat
    10981 tgacaatata agggttgcaa ttgcaaataa aaagtggagt gaagatgtga ctgtgactat
    11041 aagtgcaggt gtagctcata tttcgggtag taaaaatgta ctagaagaag ctgataaaaa
    11101 tctatataaa gcaaaacagc ttggtagaaa taaagtagta tataagtaat cgtatgctat
    11161 ataaataaga ggtataaata tgaaacaatc aatagctata gtaactgata aaataaccaa
    11221 attatcatct ttcttagaag aaaatatacg cttggtttta ggtaattata ttgatataaa
    11281 ccactattat ctggaagttt taaaagagga tgataaaata caaggtgatt ttgtactagt
    11341 aatgaatgat gatagattaa acagtatgag aaagcattta gtacaagata gtaagattat
    11401 agtgataagg agaacactta aagaaaatga aatatatgga ttattttcaa taccaaatgg
    11461 aactgaagta ttggttgtaa atgatacaaa agaaactacc ttagaaacga taagcttatt
    11521 ttacaaaata ggagtaaaaa atttaaagtt tactccatat attgaaggaa aaatttataa
    11581 aaatatagac atagctataa cgcctggagt aaaggaaaaa gtaccaagtt acataaataa
    11641 agtaatcgac cttggtaata gatatattga tatatctact tttatagaaa taataaatgg
    11701 tcttagaata gatgttaaag aaatacgtaa aaatttaatg gcatattctg agagtttaat
    11761 aagtttagat aatggagtta aagataatta taggcaactg tttttaaaaa tagaagaaca
    11821 agatgttata ttgaatctat caaaagatgg aataatattt acttcagcag atggtataat
    11881 aaacacattt aataaagaag ctttaaaaat tttaggtata aatgaaaatc cacaaggcaa
    11941 aaacatagaa gatgtcattt ctgaagattt aaatatttta cttggagaca aagaaataat
    12001 tgatgaagtg gtatttacaa ataaaaaata tataaatgta aataaaaaaa gtattttttc
    12061 tatggggaat aaagctggga tatattataa cttacaagaa ataacgtata taaaaaaatt
    12121 agaacaaaat cttactaata aattaagaga acaaggtcag atagcaaggt atacttttaa
    12181 agatataaaa actaaaaata aaaatatggt aaagtgtata gaattggcta aaaaaatatc
    12241 taaatcagac ctttctgtgt taataatagg agaaagtggt actggtaaag aattattatc
    12301 gcaatcaatt cataatgctt ctaatagaag taatcaaccg tttatagcag ttaattgtgc
    12361 agcagttcca gatagtctat tagaaagtca actgtttgga tatgaaaaag gctcttttac
    12421 aggagcttta agagaaggaa aaaagggtct atttgaactt gctaacaatg gaactatatt
    12481 tctggatgaa attggagata tgcctccatt gctacagaca aaattactaa gggtattaca
    12541 agaaaagcag gttatgccta taggttccca taatgttata aatatagatg ttagggtcat
    12601 agcagcaaca aacaaaaact taccaaagat gattagagaa ggaaaattta gagaagacct
    12661 gtattataga ctaaatgtat tgccaataga tgtaccatca ttaaggaata gaaaagaaga
    12721 tataataaca cttatgaatt tctttttgaa aaataatata agactttcgc cagaagttat
    12781 aactatttta gaaaaatata attggccagg aaatataaga gagttacaaa atgttgcttc
    12841 ttatgtaagt ctaatgtgtg atagtatggt ttttagagat gacttaccac catatataaa
    12901 ggtagaatgt gctaacaatt atgaagatgg atttggttat acaagaaaag aatttgagga
    12961 aatactgaag attctatatg aaagaagaga gtcatcctct ggagttggaa gagggtttat
    13021 aatcgaaaag cttatacagg aagggattga gatttctgaa agcaaagtta gaaaggtgct
    13081 ctcttattta agtgaatgtg gatatatatc atctaaatct ggtagaggtg gaagtcaaat
    13141 aacctcagag gggataaaac tttataataa cataaattaa gttgagtgta ttgatattga
    13201 aatcacatat tttgaattta gatatttaaa taataataaa cttttatatt tatagaaatt
    13261 aatttaaatt atgtcagaaa gaatattgta atttataatt aaatattaat aggtttttaa
    13321 aataggatta gataggtaat aggttaagat aaaataaccc attacctatt tttatttgta
    13381 agaaaagtgt atatagaatt aaaagtaaaa aatacaacta ataaaactat tgaaaacact
    13441 agataaataa taaataaaaa taattataaa ttttggcata tatattgcta atcatagaga
    13501 taagacgaca aaataattgg gaggtttagt gatgaaatac aaaacaacag ggacttgtgc
    13561 tacagaaatt gaatttgaag taaaagaaaa taaagttaca aatgtaaact ttataggtgg
    13621 atgtgatgga aatttaaaag ggctaaaagt attagttgaa ggtatgaata tagaagatgt
    13681 aatacaaaag ttaaagggaa tagagtgtaa aacaaagcca acatcttgtc cagaccaact
    13741 gtctctagca ttggaaaatt atatgaatat aaaataatac ataaaacaat cgattagtta
    13801 ggagcttaat tatgaaacca ataataggga ttttaggaaa tttaataata atggaaaatg
    13861 gtatgtttcc aggtcttgaa aggtcatatg taaataatga ttatataaat gcagtactaa
    13921 aaggtggagg tagtccagta ataattcctg taaatacaga taaagaagtt ataaaaaaac
    13981 agattgaaat ggttgatgga gtcttaatat ctggaggatg ggatgtcaat cctcaactgt
    14041 atggagaaga aacaagagaa gaaacgacat ttatatatcc agaagtggat gaatttgatt
    14101 tgattgcaat aagtatagca ttagagctta aaaagcctat attaggtgta tgtaggggac
    14161 tgcaaatttt aaatgtaagt ttaggaggaa ctttatatca agataataat ctgatagaag
    14221 gaagttatat aaaacataca caatcttcta aaagacatgt agctactcat aaagttgatg
    14281 tcaaagaagg aagtatttta gagggaattt taggaaaaca attacttaca aacagttatc
    14341 atcatcaaag tgtcaatcag cttggaaaag gattgaaagc catagcatac tcaaaagatg
    14401 gtataataga agctattgag aaagaggatg aaaattttgt agtaggagta cagtggcatc
    14461 cagaaatgat ggtagattac tgtaataaaa tggaaaaact atttaaatat tttataaata
    14521 tttgttcaaa tttataatat accttggggg gattgaacat gaacgaaaag aacaaaatgg
    14581 gattgataag tattatactt ttaggtatta atgcagtagt aggagctgga gtttttctat
    14641 taccaggaga tgcaatgaaa gcatttggtg tagcaagtat atttgtatat atatttgata
    14701 tgttgttagt tttatctatg gcattctgtt ttgctgaagt tgcaggtaaa ttcaataaaa
    14761 atggtgctgc ctatgtatat actaaagaag cttttggaga tttttgtggt tttgaagtag
    14821 gtcttatgaa atgggtaata ggatgtatat cttggggagc tttgatagta ggttttccta
    14881 catcattatc agcagtatgg gctccagcag gtgaaccaca tatacaaaaa attataattg
    14941 tagccatgat agttgggctt acaattataa acttattggg tgtatctcta tcaaaaattg
    15001 tacaaaatgt tataactgta ggtaaattaa ttccattaat actatttata ggaataggta
    15061 tattttttat aaaaggagtt aactttactt cttcaacaat ggtacctcca ggagctggag
    15121 caacagagtt tggagcagca gcacttttaa tgttttattc atttacagga tttgaatcta
    15181 tagcagttgc agcagaagat atggaaaatc cacaaaaaaa tataccaata gcaattataa
    15241 gtgtaatagt aattgcatca ataatatata tattaaatca agttgtatgt gttggtatac
    15301 taggagattc gttatctagt acatctacac cagtggcaga tgctgcaaga atatgctttg
    15361 gaaatatggg agctggattg gttacctttg gaacattggt ttctgttggt ggaatttgta
    15421 tgtgcggtgc ttttgtaaat ccaagaagct gtgtagcttt agctgatgat aaaatgttac
    15481 cgagaatatt tgcaagaaaa gataaaaaag gtacaccata tgtagcgata atagctacta
    15541 tgttaatcac tataccaata gctctatctg gaagttttgc tgaattagca gcaataagtg
    15601 cagttgctag atttatacaa tacataccaa catctttatc cgtattagtg tttagaaaga
    15661 aaagaccaga attggtaggt acttttaaaa ctccttttgg agcagtgatt cctctaattg
    15721 ctgtatgtgt tggaacatgg ttattattcc aagcatctat gcatcaatta attatgggat
    15781 taggagcttt ggcaattgga gtaccattat actttataat gaagtcttat aatagaaaag
    15841 tttatggtga aaatttaaag aaaatagttt aattatgtta taacaattta ttgatttaaa
    15901 attagatatt ggcagaaaat taaatacaaa taagtatcta taaggctata ttaaaatatt
    15961 attaattttt tattttaata tagccttcta ttgttatcaa taaatgaaaa atattgtaac
    16021 agtagaagaa caatagtatt atgtttatag acaaaaaact tattaggagg taaatttatg
    16081 ccaacaataa cattaaaaaa tggtgtagac gtacttatta gagaaggagt aagagaagat
    16141 gctcaaagca ttatagattt ttataatgaa gtgggtggag aaactcattt tctgtcattt
    16201 ggaaaggatg aatataaaat atctttggag gaacaagaaa atgctataga atcagcaaaa
    16261 gcatctgata actcggttaa actgattgca tttatagatg gtgaaatagt tgggattgca
    16321 acgattgatt ctaatcaaaa agcaaaagga aagcatgtcg gagtattagg aattgttgta
    16381 aaagaaaaat attggggaat tggattgggt aaaaggctta tgttagacct tatagaatgg
    16441 tgtaaatcaa atggaataac caagaagata actcttgtca ctaatgaaga aaattataat
    16501 gctataggat tatacaaaaa agttggattt gaagtagaaa gtatactaaa gaaagaatgt
    16561 tattataatg gtgtttacac tgatttaata gggatgtcac ttttattagg aatataatta
    16621 gtgtacattt tatctcgaaa aaagtatatt aagtatggta taattaaaaa agaatataac
    16681 ttaatataat tttggaggac gaattatgaa aaatctgtgt aaaatatcag atgattattt
    16741 aaacagcaag cttaagtatt taaagttatt atcaaaacaa tatccaagta tatcaaaagc
    16801 aagtacagaa ataataaatt tagaggctat attaaatctt ccaaagggaa cagagcattt
    16861 tataacggat gtacatggtg aatatgaacc atttgtacat gtattaaaaa atggttctgg
    16921 cgtaataaag agaaaaatag aagaattgtt ttcaaataca ataagagaca gcgaaaaaaa
    16981 gatgttagca actcttgtat attatcctga acaaaagtta gacctaataa ttaagcaaga
    17041 ggaaaatata gatgattttt atagaataaa tatttatagg cttatagaac tttgtaaata
    17101 tgcgtctagc aaatatacta ggtctaaagt tagaaaactg ttaccagaaa attttaaata
    17161 cataatagaa gaacttttgc atgagcatgt taaaagtgaa cataaagaag agtattataa
    17221 aagtatagtt gaaacaatag ttgacatagg aatagctaag gagtttataa ttgctatttc
    17281 tacagttata caaaaattgg ttgtagatag acttcatgta ataggagata tttatgatag
    17341 aggtccaaga ccagatataa ttgtcgataa gcttattgaa catcattgtg tagatattca
    17401 atggggaaat catgatatat tgtggatggg tgcagcctca ggtgaaaaaa cttgtatagc
    17461 aaatgcactt aggatatcag ctagatatgc gaatttagac attgtagaag acatatatgg
    17521 aataaatcta ttgcctttag ccacttttgc tatagaaatg tataaagatg acccttgtaa
    17581 agaatttatt ccaaaggtta atgaccaaag tgtaacaaca acagaaaagt ctttaatggc
    17641 aaaaatgcac aaagcaataa gtataattca atttaagctt gaaggtgagg ttataagacg
    17701 aagacctgaa tttgaaatgg aacatagact tttgcttaat atgataaact atgatgaagg
    17761 tacgattatt ttaaaaggta aaacatataa attaaaagat acttatttac ctacaataga
    17821 caaaaaagac ccatataaat taactatgga agaaagaaat gttattgata aattagtatc
    17881 ttcatttaga ggtagtgaaa aattacaaaa acatgtttca tttttgtttt ctaaaggaag
    17941 tatatattta aaggctaact caaatttact tattcatgga tgtgtaccat taaatgaaga
    18001 tggaagcttt atgtctatga atataatggg aaaagagtat aaaggtaagg ctcttatgga
    18061 taagatggag tctctagcaa gagaaggatt tttctttaaa gataaggcag aagaaaaact
    18121 gtatggcatg gatattatgt ggtatttatg gactgggaaa tgttcatcat tatttggtaa
    18181 agatgatatg actacatttg aaagatactt tatagcagaa aaagagactc ataaagaaaa
    18241 taagaatcca tattttaaac ttagagaaaa tgagatggct tgtaaaagat tatttgaaga
    18301 atttgattta gaacttgatg agtctcatat aataaatggt catgttcctg tggagagtaa
    18361 aaatggagag agcccaataa aagctaatgg taaaattcta gttatagatg gtggtttttc
    18421 tagggcttac caaaaaacta ctggtatagc tggatatact ttgatataca attctagaac
    18481 tttgcaatta gtatcgcatg aaccatttaa ttctgctgaa gaagccattg caaatgaaag
    18541 cgatatctta tcgacaactg ttgtagtaga gcataaggca aagagaaaaa tggtaagaga
    18601 tactgatgaa ggagtaaaga tacaagagga aatagaagat ttaaaactac ttttaatggc
    18661 ttataagaaa gggttaataa aagaaatgta gatattatag aggcacttca taattgaagt
    18721 gtctttttgt taatacttaa atatattata aagattaata atagtagttt atattatact
    18781 tactaggtga atccaagata aatgggttca ctttttttat ttataaacat tattaaaaaa
    18841 atatagttgt tttggttata ttccttgtcc tttataaata gaattatata aacaaaggag
    18901 gaggacaaga gatgaataaa ctttctgatg aaataataaa ctctctttct acaactataa
    18961 gagaaaaggt agaaaaaata tcaaacaata atctaaacat agaagaaatt aggttacgct
    19021 cacaaaaacc attgatacta aatgcaaact ctaaagatta tttttacaat caaaaaacta
    19081 tgacacttga tttaaatcaa caaaactcat atgtagtaac aagagaagat gtagagcaaa
    19141 cttttcaaat aatatgtaaa tattcaatac attcatttat ggatgacata aaaaaaggat
    19201 tcataacttt gagaggaggt catagagtag ggttagttgg aaaagcaata gtagaagatg
    19261 gacaagtcaa aaatataaag catatatcat ctttaaatat aagggtatca agggaaatta
    19321 ttggatgctc ggataagatt ttaagtcata ttataaaggg aaaaaatcag attaataata
    19381 ctttgataat atcaccacct caatgtggaa aaacaacttt aataagagat atagtaagaa
    19441 atttaagtaa tggcaatgaa gattatggat ttaaaggttt aaaagtagct ttggtagatg
    19501 agcgtaatga gatagcagga gcttatctag gtattccaca aatggatgtg ggaataagga
    19561 ctgatataat agaaacttgc ccaaaagact tgggtattac gatgctttta aggtctatgt
    19621 caccaaatgt tattgtgaca gatgaaattg gaagtgaaaa agaaataaag gcattataca
    19681 cagcattaaa tggaggtata ggattaataa ctactgtgca tggagattca atagatgata
    19741 tacaaaatag aaaagaatta aatagacttc tggataaaga gttatttaaa aaggttataa
    19801 ttctttctgc aaaaagagga gcaggaacga ttgaaaaaat ttatgattta gaagaaaaga
    19861 gatggtattt tgcaaattaa aataattatt atagctttct tgataggaag tagttatttg
    19921 ataggtgaac aaatatacaa aacttatact cgtagacata aacaattaaa cgatttaata
    19981 agagttttag aaattttgag gatggattta tcttttggac tttatacatt agaagaaatc
    20041 tttaatagaa taggtggaaa taaagagttt tgtttttgga aatttttcta tcaaatatca
    20101 gaaggacttc ataatgaaca aagtaaaact ttggaaataa taatatcaga aaatattgat
    20161 gtattaagta aagaaactta tctaggcaat aaagaaattg aggaactgaa aaatctaata
    20221 cttactttag ggaaaagcga tatagagtca caacaaagaa tgatagattt atctatagaa
    20281 aatttgaaaa aacaaaccta tgagacaaag gaagatataa acaaaaaagg cgtattgtat
    20341 aaaaaattag taacattcat aggtataggt atatgtatta tattgattta atatggggag
    20401 gtttattatg gaaatatcat tgatattaaa ggttgcaggt gtcggtattc taatatcagt
    20461 attgaatatg atattggaaa aaacagatag aaaagattgg gcaggtctta ctacactagc
    20521 aggtgtaata atcgtattgg gtatggtaat aacagaaata agtgacttgt ttaatactgt
    20581 aagaacaatg tttcaacttt actaaagagg agggagtaat ttggaaataa tgcaattaat
    20641 aggaattgca atcatttcaa ctacactctg tttagtaatt aaaaaagata gaccagagat
    20701 agcaaatttc atagcaataa taactggagt aataatacta ttgtcagtta tgtttaagct
    20761 aaattttata gtagatagta tacaagattt ggctaacaag gctaacatcc cgactatgta
    20821 tatatcatta ataataaaat taattgggat agcatactta atggaatttg cgatacaact
    20881 ttgtaaagat tgtggggaag gaaacattgc ttcaaaacta gaatttggag gtaaaataat
    20941 agttatgtca atgtctttcc ctatactttt gtccattgta gaaatggtgg tgaatataat
    21001 tccatagatg tgtatcttgg aatgactata agtatattag agagctttcg ttgtattgag
    21061 gtttatagag tgaagagagg catggttgct tatgaaaaaa agatttttac caatgattat
    21121 aggtttttta tttactttct tttttgtaaa tatctttgct attatgatat ttgctaatga
    21181 agtaccaagt agtgaagata aagaatatgg tgaaacaaaa aatagcatag ataagtatat
    21241 agatggtcaa ttagacaagc tagatataaa tgagattcaa gactatataa ataaagaaat
    21301 agttattaat gacgttaatt taaaatcatt tgtaaaagat ttaattagtg gagaaaaaaa
    21361 tatcttagat ttattcaata aagatggatt gaagatattg atgtttgatg aatttaaagc
    21421 aagtttaaag gtagtagcag taattttggt actggctctg ttatcatcta ttttaaagag
    21481 tctggaaaac tctttttcat caggtgcagt aagtcaaata gcaacatata taatatttat
    21541 cacaatggta tcacttacac ttgttggttt taaagatgta ttacagattt gctatgatgc
    21601 aatagaccat actgtaggtc ttatgcaagt tataatgcca atattaataa catttttact
    21661 tttaattgga ttcccaataa catcaactac tttgaatcct atcttcatag gaggagtaac
    21721 attcataaat gtatttttta aaaattttct atttgtatct attactgttg catttgggat
    21781 tttaataata aacaatttat caaaaaatat aaggctaaag agatttttct cctttgtaaa
    21841 acaaataaat tatgtttcta ttggagctat gtttactgtt tatttaggtc ttgtatccat
    21901 acaaggattg tatgtaacca gttttgataa attcagtgta aagactgcta aatttgctat
    21961 aggtaatttt attcctgttg taggtggatt tgtatctgac tctgtggata ttttattatc
    22021 ttcatctcag cttataaaaa atatatttgg aggaataggg cttatcttac ttgttggaat
    22081 ttgtttactt ccagtaataa aaattttgtc agtaatagta gtatacaaat tagcagcaat
    22141 aattgtagag cctgttggag aggatggaat atctaatttt ttaaatgaag tggcaaattt
    22201 aatgataata atgttagcat cagtgattgc tataacagta atgttctttg taactgtagc
    22261 tatcttgacc tcgataagtg tcgtaagtca agggtaattt atatatcagt gaaaaataat
    22321 tacatttaat ctaatgtttt tagagaggag gaagctatgt tagagggcat aaaggcatgg
    22381 atagttagtg ttttgatagg agcatttata gtaaatatag tagatatgat tttaccaagt
    22441 tctaagataa aaccttatgt caacctagtt ttaaacttta tgtttgtatt tattgtaata
    22501 actccagtag taggattttt ttcaaaagat atgagtttag aagatagaat attaaaatct
    22561 atgggaagtt acaataaaca gtatgtagat agtacaaatg ctttggcaaa agagactgga
    22621 aataatagtc tatccaaggg atatgaggat gggctaaaag aagtacttaa attaaaactt
    22681 gatgaatatg gttatgattt agaagatata gagcttaatg gagcaaatat aaataatata
    22741 aaaataaaag aaaagaataa tagtgcaaaa agtaatagta gttccaatga agaaaataaa
    22801 aataatagta ctaaaagaga ggaaaaaaac ataaactcta atgacaaaga aaattctaaa
    22861 caagtcttca aaaaaggtac agagtatggg ttgaacctaa atgaagaaaa attaaaaaat
    22921 gacttaataa aagtgcttga tgtttctata gaagatatac aaattgataa ataggagttg
    22981 ggagaatgtt gaaaaactta aatgacaaag ataagagaaa ggtatattca ctaataacta
    23041 tagcaggaat atgtgtggtt tcattagtat tattatcttg ttttcctagt agcaaaagcg
    23101 ataaacaagt tggaaaaaca gagactaata aaaatgctga aaagcaagtc actaaagaac
    23161 aagaaaaaga tgatttagag tcaaaattaa cagcaatact atctaaaatt gatggtgcag
    23221 gagacgtcga tgtaatggtt acatttgaat caagtgaaga aatacaacct gcttttaatt
    23281 caaataatac aacagaaaca acagaggaaa aagatgcaca aggtggagaa agaacagtta
    23341 cgacttctag tgaaaataaa acaatgataa cttcaaattc aagtgaccct gtagttataa
    23401 aaactacaga gccaaagata aagggagtga tagtggtagc aagtggagca agtgacccaa
    23461 gtgttaaaga gacactttat agtgcagtac aaacatcatt acaagtagct ggtcatcaag
    23521 tagaaatata ttctaaataa atacatataa ccggggggat aaatgaatga agtttaatta
    23581 taagggaaga ggatttgtaa taataacttt aactgcaatg ttagtggtag taggaacagt
    23641 gaattatcaa ttaagtaaga aatctttatt agaaacatca aaagagttta aggcatatga
    23701 agaagcacag ttacaaaaaa atactgatga tggtgatagt agtaagacag aggattctag
    23761 taatgtagat aaacaagata gtaaggaaag tgctgatatt gatatagttg atagcaaagc
    23821 tagtaaagta aaagaaaagg caactgagac aagtaaggaa ataaaagctc aattatcatc
    23881 agagaaaaat atgaaaaagg catcttatat tttagatatg aaaatgaata gagaaaaaca
    23941 aagaaatgaa ttagttcaag atttaaatga aatgataaat aatccatcta caacagaaga
    24001 atctagaaaa gaagcttcta atatgaaatt aaatatagtt aaaactcaag aaaaagaact
    24061 tcaaatagaa aatcttttga gtacaaaagg atatgaagaa gcattagttt atataagtga
    24121 taacaaagtt aatgtagttg taaatgaagc taaattagaa aagaaagatg cagcaaaaat
    24181 atttgattta gttgcagaac aagccaatgt aaagtatgaa aatattaaac ttacaaataa
    24241 taatagtaat aagtaaagta taaacctcag aattaactga ggttattttc ttgcaaagta
    24301 gacttgtatt tggtattatt atacttataa aaatagttcc ttaaaatcag gaggtaagtt
    24361 ttaatatgga agataataaa tttggacaag taaaaatatc taatgatgta atagctacaa
    24421 tagctggttt agcagcatta gaagttgaag gcatagaaac tacagcaaca ttaacagata
    24481 agttgttaaa aaataatgga gtaaaaatac aaatcgaaga agaggatgtt aatttagatg
    24541 taatggttac gataaagtat ggaatgtcaa taccagatac agcttttaaa gttcaagaaa
    24601 atgtaaaaaa tactgttgag acaatgacag gactgaaggt ttctcaagta aatatacaca
    24661 ttcaaggaat cagctttaag aaggataaag ttgataaaga agaagcaaaa gcaactaaga
    24721 aaaattaata ttagccctct gaaaagtcag agggtttttt gctggtaatt gattttatat
    24781 ggataattaa acttaaagga gaattttgat tgatgaaaaa agatagggca caaaagagta
    24841 caacaagaga atatataatg aaacttatat accaaataaa tattaataaa gaagattttg
    24901 aaactttaga agataaagta gataattttt taaaagataa ttcagaacat attataaata
    24961 gatataaaga gcttgcactt caatactcaa aaaatacaaa tttaaagtta gaagatactg
    25021 aaattgaaga tgttatagac aaaaaatata taaatacagt atgcaaagca ttgaaagaaa
    25081 atcatgacaa aatagatgag ttaataaata agcatgctaa aaactggaca gtagatagaa
    25141 tgccaaaggt agatgtttct atacttagat tgtctgtgtg tgaaatactt tatttagaca
    25201 ctcctaataa agtatcaata aatgaagcag tagaacttgc aaaaatatat tgtgatgata
    25261 agtctcctaa atttataaat ggtatattgg gaagtgttgt tgatgaaatt gggaaataat
    25321 ataataattg gaattgatac tagctgctat actacttcta tagcagctat ctctttagat
    25381 aagaaagtta tattcaatga aaaaataatg ttagaagtaa aggataattc aaaaggttta
    25441 aggcaaagtg aagctgtatt ccaacatatc aataatttag ggattcttag tgataggata
    25501 aaatctttta aagataagtt taatgtagaa ggagtttgct catcaaaaaa accgagacct
    25561 gttgaaaact catatatgcc tgtattcaat gtaggtcaca attttggaaa attattgtct
    25621 agtatatatg gttgtaggtt ttatgagact acccatcaag agaatcacat tgaagcaagt
    25681 ttattaaata gtaaattaaa aaataataat agatttattt ctgttcatat gtctggtggt
    25741 actacagaga tacttttgac aagcaaacaa gatagtcatc acaatgtatg tgatactaat
    25801 ttaggtaaaa ttgccaaaat aagtattaaa aaagatgata aaagtaagtt atacaataat
    25861 tttggatata atatagatat aattggagga agtaaagata ttagttttgg tcaacttata
    25921 gatagagtag gaattaagct tggatataaa tttccttcag gaaagtattt ggatgaaaat
    25981 gctttaaatt gtaatcttaa aatagaaagt ggtctgaaaa cttctgtaag agatgggtat
    26041 atgaatttat ctgggttaga aaatcaggta aataagatta taaatgataa tggagataat
    26101 actaatcaaa aagaatacat atctaaatta gtattagatt cagtagttag aaatatgttt
    26161 aaatctttag tatatttatg tgaaacttat aatgtaaatg aagtagtatt tgctggtgga
    26221 gtttctgcaa gtaagtatat tcttagagaa ttaagcatga agttaagaaa aaagcatata
    26281 gaagcttatt ttacagaacc acaatattca actgataatg ctgtcgggtg tgctataata
    26341 ggattgaata actttttagg agaaagagta tgaaactaag agctttagat ataagtgaag
    26401 caaactcata tattaaaaga attttaataa acgacccaat actttctaat cttaaagtta
    26461 aaggagaaat ttctaatttt aaggttcata gtagtgggaa tgtatattta tcacttaaag
    26521 atgaaacatc aaagttaaac tgtgtaattt ttaaaagtaa ttttaatcgc aatttaaaat
    26581 tagataatgg tgtaaaagtt atagctaatg gatatatatc agtctatgaa agagatggtg
    26641 cgtatcaatt atacattaat gaaattgaaa tagagggaat aggaaattta cacatagagt
    26701 ttaatagatt aaaggaaaaa cttaataaag aaggattatt tgaccctaaa tacaagatac
    26761 ctatacctaa aatgccaaac tcaattggcg ttataacatc tccaacaggt gctgttataa
    26821 gagatataat aaatgtaata aaaagaagat atccgaaagt taatataaag ctttatccag
    26881 taacggttca aggagataaa tcagctgaag aaatctgtga agcaattaga tttttcaatc
    26941 atatgaagaa tgttgataca cttatagttg gtagaggtgg gggttcaata gaagaacttt
    27001 ggtcttttaa tgatgaaatg gtagcaagag aggtttttaa ttctcaaatt ccgataatat
    27061 cagcagttgg gcatgaaacg gattttacta tttgtgactt tgtgtctgat atgagggctc
    27121 caacgccatc agcagcagct gagattgcta ctccatcatt ggatgatata aactataaac
    27181 ttggaaacat aaaaagcagg atgagtaagt ctcttacaaa tcaaatagag ctagaccaat
    27241 ataggttaga aacagtattt aataagataa ataactattt agattcatac acaataaaag
    27301 ataaagttat acaattagac aaaatatatg ataaaataat ttttggaata gaaaataatt
    27361 taaaattaga agatgaaaaa ttagtaaaaa ttggagcatt actccataat ttaagtccat
    27421 tagctactat ggatagaggt tatagtataa ctcaaaaaaa tggaaaagta ataaatagta
    27481 taaaaggatt aaaaataaag gatagtatag atatagtatt aaaagatgga aatttagaat
    27541 gtatgataga taaaattgaa aataaagagg gatagtatga atttaaccta cgaagaagct
    27601 tataaaagat tagaaagtat tttaagtgaa ttagaatcta aaaatgctag cttggatgaa
    27661 tctttaagcc tatatgaaga aggtataagc ctttataaac attgtaataa gcttttagat
    27721 gatgctaaat tgaaaataag taaatttaat caattaggaa tagaagaaga ctttaaaata
    27781 gaggaggaat aaaattggaa tttaaacaat gtctaaaaga aaaagcaagt tttgtagaaa
    27841 aagtacttaa agaatatatg cctaaagaag aagggtatca aaaaactgtt attgaagcta
    27901 tgaactatag tttaagtgct ggaggtaaaa gattaagacc aatacttaca ttggaggctt
    27961 gtaaaatagt tggaggaaat gaagatgaag ctattccttt tgctattgca attgaaatga
    28021 tacatacata ttcactaata catgatgatt tgccagctct tgataatgat gatttaagaa
    28081 ggggaagacc aacaaatcat aaagtatatg gagaagctat ggggatttta gcaggagatg
    28141 ctttgttaaa ctatgctttt gaagtaatgc ttgcaggctc aatcaataaa gaaaatccag
    28201 aaaagtatct aaaagctata aatgaaattg ccaaaggtgc tggtatatat ggaatgatag
    28261 gtggtcaagt agtagatgta gaaagcgaaa ataaacaaat tgaaaaagaa aaattagatt
    28321 atatacatat gaataaaact gctgcaatga tggtaggatg tatgagagca ggagctacta
    28381 taggtggagc aaattttgaa caaatggaag aaataactaa atatgcaaaa aatatcggat
    28441 tatcattcca gatagtagat gatatactag acatagtagg agatgaagct aagttaggta
    28501 aaaaggttgg aagtgatata gaaaatcata agtcaacata cccatctctt ttaggtttag
    28561 ataaatctaa agaaattgca cataatttga ttgatgaagc aaagaaaagt atagaaaaat
    28621 tatctgatga tgtagatttt ttaaagggat tggctgaata tataatagat agagaatact
    28681 aaaatataca gctaaaatcg aggagggaaa aatggatttt ttttcggaga tttttaacaa
    28741 tggggctttg ggaataagtc tgattgcttg tttcttagca caatttataa aaatatttac
    28801 tggtaaggaa aaaagaattg agttatcaag aatacttatt tcaggaggta tgcctagttc
    28861 ccatagttct tttgttacaa gtttagctac agtggtaggt attgaaaaag ggtttaattc
    28921 tactgatttt gctataatta ccgtgcttgc cttaattata atgtatgatg cagcaggtgt
    28981 tagacgggct gttggtaaac aagctactat attaaatcaa atggtggcag atatacagca
    29041 tggaaagcat atagagcaga agaaattaaa agaattaata ggtcatacac cactagaagt
    29101 ttggtttgga gctttacttg gaatagtaac agctttaata ttgatgtaaa ttgaaaattt
    29161 acagaataaa ggtgaggata atatgtataa atatttagat aaagtaaatt ctccaaagga
    29221 tataaaaaat atgagtatag aagaaatgga tttacttgca aaggatataa gaaagttctt
    29281 agtaaaatct gtttcaaaga caggaggaca cttagcttct aatctaggag tggttgagtt
    29341 aacattagca cttcataaag tatttgatag ccctaaagat aagattgtat gggatgtagg
    29401 gcatcaatct tatgttcata aaatagttac tggaagaaaa gattgttttg tatctttgag
    29461 acagtttaat ggactaagtg gttttccaaa ggagaatgaa agccctcatg atatttttga
    29521 tacaggacat agcagtacat ctatttctat agctacaggt attgcctgcg ctagggatat
    29581 aaaaaaagaa aattatagtg ttatatctgt gataggagat ggttctataa caggaggtat
    29641 ggcacttgaa gctttaaatc aattagggta tatagatacc aatatgatag taatccttaa
    29701 tgataatgag atgtccatag ataaaaatgt tggaggaatg tctaaatatc tgtcaagtat
    29761 tataagaaac tcaacagttg agaaaatgac agatgaagtt gataaaattt taaatgttac
    29821 acaaacaggt gaaattttat ctaagactgc acatagattt aaagataaac taatgtatag
    29881 cttttctcct caggactgtt cattttttga ttcattaggt ataagatatt atggacctat
    29941 agatggacat aatactaaag aactaataga tatattgaga aaagctaaac acaaaaaagg
    30001 gcctgttctt ttacatgtaa ttacgaaaaa aggaaaaggg tacagatttg cagaagaaca
    30061 acctgataaa tatcatggag tatcaaagtt tgatataaag acaggtgtta catctgcaaa
    30121 agttaagtct atgtcaatta gtgttggaga aaagcttgtt gatatggcta ataataatga
    30181 agatatagta gcaataacag ctgcaatgcc atctggtaca gggctaaacc tatttgaaag
    30241 tgcatatcca aaaagatatt atgatgttgg tatagctgaa cagcatgcaa caggatttgc
    30301 agcaggtctt gcaaaaaatg gtatgaaacc ttactttgct gtgtactcat catttttgca
    30361 aagggcatat gaccaagtta ttcatgatgt atgcataaca aaaaaaccag ttacttttct
    30421 tatagataga gctggattag ttggaaatga tggagaaact catcatggta tgtttgattt
    30481 aagttactta aattctattc caaatattgt agtaatggct ccaaaagata caagagagat
    30541 ggagcttatg atggatttgt cattaaaatt agattgtcca ctagcaatta gatacccaag
    30601 aggaagtagt tattacctag ataagggaga atatggagag attgtactgg gtaaatatga
    30661 ggtattagat gatggtcaag atacagtcat actttgtatt ggaagtatgg taaaacatgc
    30721 cttagaggct aaagaaatat tatcaagaga aggaataaat ccgacaatag ttaatgctag
    30781 attcttaaag ccaattgatg aagatatgtt aaaagtatta ttaaaaaatc ataaaaatgt
    30841 agttactata gaggataata ttgtaactgg aggatttgga agtagaataa ataagtttat
    30901 tatagataat gaatataacg taaatatact taatatagca ataccagaag aatttgtaaa
    30961 acatggcaat gcagatgaat tatatgattt tgtaggatta tcgccaaaaa gtattgctga
    31021 taaaataaga aaattagtta ttgagtaaaa atctaatgta gatattgtaa attaaaactt
    31081 atataaaata tagaatttat caattagaat aaatatcaaa tgtgttactt caagctactt
    31141 tagttatcat taaagtagct tttaacagaa aggtaattgt atgaaaaaaa gaatagattt
    31201 attattagta gaacaagggt actttgaaag tagagaaaga gccaaaaaag caataatggc
    31261 tggattggtt tttgttgata atcaaaggtg tgataaggct ggtacagaag taaaagaaga
    31321 ttgtagtata gaagtaaaag gaaatccaat accatatgtc agtagaggtg gtttaaagct
    31381 tgaaaaagca atgaaaaact ttgatttgac tattgatgga aaagtatgta tggatatagg
    31441 ggcatccaca ggtggtttta cagattgtat gctcaaaaat ggagcaataa aagtattttc
    31501 tatagatgtt ggttatggtc aacttgcatg gaaattgaga caagatgata gggttgtgtg
    31561 tatggaaaga accaatataa gaaatgtgac aatagaagat acaaagcagt ttgctgattt
    31621 tgcgtcaatt gatgtgtctt ttatatcatt aaagttagta cttccaaagg caaaggagtt
    31681 agtacgtcat gatggggaaa ttgtcgcttt aattaaacct caatttgaag caggaagaga
    31741 aaaagtaggt aaaaaaggag tagttagaga aaaatctaca catatagaag ttataaaaat
    31801 gatttctgat ttctcagtcg aaaatggatt tgagatttta gggttagatt tttctcctat
    31861 taaaggtcct gaaggtaata tagaatatct aattcatttg agaaatggaa atgaaggata
    31921 tgaatttgat ggtgagatat acaataacaa aatagtagaa gttgttgaag cttctcataa
    31981 tttagacaaa taggttaaaa gggggcttag atttgatcct cgaattatat atgaaaaatt
    32041 gtgccttagt tgaagaacta aggttgaata ttgataaaaa cttaaatata cttacaggtg
    32101 aaactggttc aggaaaatct ataataatag atgcacttgg actttgttta ggtgataaat
    32161 atgataggtc ttttttaaga aaaggaacag ataagggact tgttgaagct gtattttttt
    32221 cagacaatat atatcttaaa aaaatattag aagaaaatga tataagtatg gaagatgata
    32281 atttattggt aataactagg ttgatatact cagatggtaa gagtactgct agagtaaatg
    32341 gaagaactgt taaagtgtct cttcttaaag aaattgcatc tacacttata gatatacatg
    32401 gtcaacatca aaatcaagct ttatttaata aagatactca tttaaaattt ctagaccttt
    32461 ttggagaaaa tgaattggaa gggtttaaaa ttgcttataa aaatgtgtac cataagtata
    32521 gtgaagtaaa aaaagcttta aattgcttga cagaaaataa agatgagatg caaattcaac
    32581 gagaaataga tttattaaga tttcaaataa atgaaattga agctgcaaat ctaaataaga
    32641 atgaatatga agatttatta aaacaaagag aagtttatag aaatagtgaa aagatatata
    32701 ataatctaaa ttcgagttat agcaagttac ataatggtga gtataatgtt attgacctaa
    32761 taggtcttgc gtctaaagaa cttaatgaca tatctaaata tgacagtgtt ttaagtgaat
    32821 atagtgatac tgtagaaaga attatgtatg agttacaaga catatctggt gagataagaa
    32881 attacaaaga taatatagac tttgaacctt atgagctaga gcagattgag cttagaatcg
    32941 acgagattaa taacttaaga agaaaatatg gagacagtat agatgatata tttgagtatt
    33001 atggaaaaat taaagataga ttagatgaaa tattaaatag agatgaaaga gttgaacagt
    33061 taaaaagcaa actgatgaat atagaagaag atttaaaaat aaaagccagt aaattgacta
    33121 aagcaagaat agaagtagca actactttag aaaaaatact tcttgatgaa ttaaaaagct
    33181 taaatatgaa aaatgtaatg ttcaaggtta attttgaaaa aggtctattt actttagatg
    33241 gagtagatga tattgaattt atgatatctt ttaacttagg agaagatatt aagcctatat
    33301 ataaagttgc atcaggtggt gagatgtcta gatttatgtt agcatttaaa accatattgg
    33361 ctgatataga tgatattgat acattagtgt ttgatgagat agatactggg ataagtggga
    33421 tagcagcaca gattgttggt gagaaattga gtgatatagc aaagaaaaaa cagataatat
    33481 gtataactca tttacctcaa attgctgcaa atgcagacac tcattattgt atagagaaag
    33541 atacttcaaa taataggacg tttaccaatg ttagtaagtt aaatgaatct caacgaaaaa
    33601 atgaaattgc cagacttata gcaggtaaca atataacaga aaaaactatt gaacatgcaa
    33661 gtgaaatcat tgaattggct aaaaaatgtt agtgaaaaac aagcatttta ataggtgtaa
    33721 agaattttta tagaaatgct ttacacctat ttttttatga tgaaattatt ttaaaatatc
    33781 tagtgcctat attgaagaaa agtaggtttg aaaatggaat gaaatttgta taaatttgta
    33841 actttgaaat ttctaaatgc ttaagtataa tattatttat taaggtagta gtgaggggag
    33901 gcataaaatg aataaaaaag atactatgca cttaattttt ataattgtaa tttttatttt
    33961 attatggtat attgtggtag cagtaagtga gattaataaa aatattgaaa gttgtagtaa
    34021 caaattagat tatttatcac aagagattat gagcactaag tcggatattg gtaatactat
    34081 aaatgaagaa atgtcaaaaa gttatataac aaaaagtatt gacttaaaag ttaaaaaaat
    34141 cgagaaaaaa gagtgtatta ttgatataga tgttcaatta agtaaacttg gaaaaaatgg
    34201 aaaagtattc tttatgtata aagaagatag tgataaatgg aatgaaactg agatgacaaa
    34261 acatggagaa ttatcatatg catgtgagat aaagataaat acaggaagtg aatatgacta
    34321 taaagtagtt acaagtggag ctatatctga aagttctgat gtgcaaaaat tgactaaatc
    34381 tgattatatg cctgaacaac ccatatttaa ttctgggata agagataata gagaatactt
    34441 tattgaaata gtagaaaata gtaatctttt caatcatgag agtgaaaagt caaaaaataa
    34501 agcttatgat aatattagat tagaaaaagt agatattatt gtaaatgaag gaaaaaaaga
    34561 cactatatac aaagccaaat tagctgaagg cttagatgat ggcaaaacta ataattcaaa
    34621 tagaaatcaa gtgaattatg aagtaagttt tcctaagagg gatattgatg acataattta
    34681 tattaaagct aaacttatgt acaataatgg agtagaatat acaaaggata taactgaaga
    34741 tataactatg ggattacaag atttagaaaa ataaatacat atttaaataa atattcaaat
    34801 agatttgttc aataaaatag agtataagta aaactgtagt agttttacct atactctatt
    34861 aaaaaattat aattttaatt ccatatatct agccccttca attggattat aagtataagg
    34921 ttctatatca taaaaaccaa acttttcata taatccttgt gcacttttca tgcttggaag
    34981 agtatctaat ctcatgtgtg tatatccaat ttttttagct tcttcaatga tttcttccaa
    35041 tagtattttt cctattttta atcctctaaa ttgatttctt acatataatc tttttaattc
    35101 acatatttta tattcaagtt tttttaaagc aacacaacct gccaaatttt catcaacaaa
    35161 tgccaatatc aatgagcctg aaggtttttt atattttcct ggaagtgttt ttaattcatt
    35221 attaaagtct tggaaacata agtctatatt tagagaattt gaatattcag taaataaaag
    35281 ttttacgttt tcaaggtttt ctaaatcttt ttcttcaaca aattgtaaag ttatattttg
    35341 agccatttta tccgccatta tataaacatg tttatataat gacttcacct ccttgatata
    35401 tgtatatttt tcttaatatc aattagattt taacaccatt actttttcac caccatagtc
    35461 aagctctcca ttaaactcaa atccaaaatc tttataaagc ttaatagcca ttttattgtc
    35521 ttcgaatata ctaagatata gttcatcaca gttatattca tttttaagat gatttacaag
    35581 aaaatctaat gatgcttttc catatccttt accttgatat tttgaatcaa tcataaatct
    35641 atcaagccaa actctatcat cttctaaacg accatacata gcaaatccta ttaaagaatt
    35701 ttcatcatat attccaacag gtatccaagc tgttataaat ttagattcag caatagaaag
    35761 tgcattgctt tctatataat ctaactgact tttatctaca gacaaactaa ctaaatcatg
    35821 ccaattattt atgtctgcaa ttcttacatt taatttcaag atattaccgc ccttcaaact
    35881 ttttgttaca atataatact atcatattat gtattaaaaa tcacttatta agattttaaa
    35941 taaacttttt gaatgaataa attctcagga aaaaggaatt ataaatacat aataatacag
    36001 cataggagta gatggatata tgaacaaaat aaataaaaat ttcaaaaata tttggataat
    36061 attaggagct gtatcgattt ttaccatggt tataattttt aataaaaatt gtaatgttaa
    36121 taaagaaact caaatagaag cttttacaat taagaatcat gataaaaaat ttgtatatcc
    36181 tatgggaaat ataattggag taaaagcaaa tacagatggt gtattggtct taggatatga
    36241 ggaagaggat atagactata ttggaggcat acaaatcggt gataatatag taaaaataga
    36301 taataaaaga ataaaaaata gtcaagatgt atcagagatt ttgaataaga ttaaaaaaaa
    36361 taaagttgaa gtgacttttg agagaaaaaa taagtataaa acagaagtaa tagagactaa
    36421 aaaagaaaat ggcaagtata aacttggatt atgggtaaga gataaaatat ctggaattgg
    36481 aactatgact ttttacgacc ctagtatgga aaagttcaaa gcaatcggtc atgccataaa
    36541 agattcggat actaatgaac tattaaagat aaagcaagga tatatatata agccagaaca
    36601 attaaaaata gtaaaagcta gtaatgaaaa agtaggtaaa ataaaaggag attttaatga
    36661 cagcaattta atgggtaatt tctcaaataa ttcagagcta ggtataagtg gtaatattat
    36721 agaaaaccat aataaagaat ttaatgtagc aaataaagaa aagcaattaa tagaagttgg
    36781 aagaccacaa gatgtaaaaa taggagatgc agtaattctt tttgaggata aaaacaaaaa
    36841 tataacaagc tatgatataa aaatagaaag tatagtgtat gataaaggaa attatagaga
    36901 tatggtaata aaagtagtag atgacaagtt attggaatac acaggaggta tcgtacaggg
    36961 gatgagtgga gcaccaataa tacaaaataa taaaattatt ggtgcaataa ctcatgtttt
    37021 tagagataat ccgaaaaaag gatatggtat ttttatagat gaaatgataa aattgtaggt
    37081 gaggcattaa aaaattttat tattttatca attatctagg aggaatataa ttttggagtg
    37141 tcgaatatgc tttagagtag ataattagga agcaattgtg taaaaagttt agttttctgt
    37201 aataagaaga tgttttttaa tggggggatt tttagtggaa aaaatcaaaa tagttttagc
    37261 agatgacaat aaggattttt gtcaggtatt aaaagagtat ttgtctaatg aagatgatat
    37321 cgatatatta ggcatagcta aggatggaat tgaagcatta gacttagtaa aaaagacaca
    37381 accggactta ttaatactag atgtaataat gccacatcta gatggattag gtgtaattga
    37441 aaaattgaat actatggata tacctaaaat gccaaaaata atagtactat cagcagtagg
    37501 tcaagataag ataactcaaa gcgcaataaa tctaggagca gattactaca tagtaaagcc
    37561 atttgatttt gttgtgttta taaacagaat tagagagcta gtttctaata gagttacaca
    37621 agttgagcca aaacctagac cagttcaaga gactcaaatg actagaagtg attttgttaa
    37681 gaatgtagga aatatagaga cagaaatcac aaatataata catgaaatag gagtaccagc
    37741 tcatataaaa ggatatttat atttaagaga agcaataaaa atggttattg ataatgttga
    37801 gcttttaggt gcagtaacga aagagttata tccaagtata gctaaaaaat tcaatacaac
    37861 accaagtaga gttgaaagag caataagaca tgcaatagag gttgcgtgga gtagaggaaa
    37921 agttgacaca ataaatcaat tatttggata tacggtacac aatactaaag gaaaaccaac
    37981 taattcagaa tttatagcaa tgattgctga taaattaaga ctagaacata gtatggttaa
    38041 ataaacaaga cataaaaagt aaggcttttt aattaaggca ttggctataa atgcgtatta
    38101 caagcagcga aacggtataa ccactagggt tataccgttt cgctatttta ataaatataa
    38161 aaattttctt tattatttgc ttactatatc aatataataa ttttattata ctatggatat
    38221 agtatgtgtc tttacaagtt gtaaactgac agtggtttat tttttaatat aaatattgac
    38281 tttgatgtag gtaaactttg catttttaag catattatgg aatatgttaa ataaaaaaat
    38341 gatgaaatat agtattgtaa atgccaaaga tgcgaaacaa acttaaaaca tttattttat
    38401 tgttaagtaa tgctataata taatgtgatt ttaataatga tagtggaggt ttaaatatga
    38461 gagtcgaggc ccctataaaa gtagatcgaa aaaccaaaaa acttgctaaa agagttgaaa
    38521 gtggggaaat agcagttata aatcatatag acatagatga agttgctgca aactctttag
    38581 tagaagctaa aataaaactt gtcataaatg cggctccttc tataagtggt aggtatccca
    38641 ataaaggtcc agggatatta actgaaaata atatactaat aattgataat gttggagaag
    38701 aattatttga agaattaaaa gaaggagaaa ctatagaagt tgtagatgga aagatatata
    38761 gaaaaggaaa atttttaggt gcaggagaag ttttagacaa gtatgaggtt tcacaccaaa
    38821 taaaggctgc ctatgagaat ttagctgtag aattggatag atttatagat aatactatag
    38881 attatgctaa aaaagaaaaa ggatttatac ttggagaagt agaaattcca aaagtgaaaa
    38941 ctaattatgc taataaacat gtattgatag ttgtaagagg tcaggactac aaagaagatt
    39001 taagtactat gctttcttat atagaagagg taaagccagt attggttggt gttgatggag
    39061 gagcagatgc tttaattgag tttggatata ctccagatgt gattgtaggt gatatggata
    39121 gtgtaagtga tgaagcttta aaaaaggcta gtgaaatagt agtacatgct tatacagatg
    39181 gaagagcacc aggtcttaag agggttgagg aattaggctt agatgcagtt gtatttcctg
    39241 cgccaggaac tagtgaagat atagctatgc ttatcgctta tgaatataaa gctgagttaa
    39301 tagtagcagt tggtacacat tcaaatatga ttgattttct tgaaaaagga agaaaaggta
    39361 tggcaagtac ttttttagtt agactaaaaa taggttcaaa gcttatagat gctaaaggag
    39421 ttaatttgtt atacagaagt aaattaaaaa taaagtacat atgggcatta atagcaacag
    39481 cattatttcc tgtgttggta gttgcatcat tgtcaccagg agttcaacag ttcatacaac
    39541 taatgcaatt aaagtttaga gtattattac agatgtaaat tttaggagga taaatatgca
    39601 tattaatatg aaatattata tagtcactat aggggctata tttatagctt tgggtatagg
    39661 catgttagta ggatttaatt taaataataa tcaacaatta agtgaacagc aagcaaacat
    39721 aataaatgat ttggatgata agtttaatat cttaaaagaa aaaaatgata aacttgacag
    39781 tgacttagca agtgtaaata gagattatga agaggcagtt aattttataa ataaaaatgt
    39841 agataagatt ttagctggtt cgttgaatgg taaaaatata ggaataatat cggctaatga
    39901 aaatgacgat tatacaaaaa atatagaaga cataataaat aaatctaatg gaagtattgc
    39961 atttaatata gtattaaaag aaaatataac aaatccagaa aagttaaagg aaatgtcaac
    40021 taaactaggt acagatgtaa aaaatgcaaa tgatgcagtt aattatataa ttgacactct
    40081 taaaaaggaa gatgcaagtg acattttaac atatcttcaa gaactagatg taataaagtt
    40141 taactttatt ggagatacat atctaaaata tgattctgtt gtaatagctg gtggaaatga
    40201 tgcaaaagac aatacaaaac agtttgaaaa aatagaaaaa tttgtagttt caaaacttaa
    40261 atcagagaat aaatatttag tagaagttca aaatacagga gttaaaactt cttatgtaga
    40321 actatattct aaaaacaaag ttgcaactat agacaatatt gatgaaggta taggaagtat
    40381 atctctagct attcttctac aacaaggtaa tatagttggt aattttggta gattggatac
    40441 tgcaacttca ttgttgccat ctattaaata gaaataattt taaggagaga aaaaatggat
    40501 tcttatataa gtataataat accagcttat aatgaagaaa gtagaataaa atatacatta
    40561 gaaagcatat tggacattaa acaaataaac gaaataatag ttgtggatga tggttcgatt
    40621 gacaatactg ctaaagttgt atctaaacta gaaaatgaaa agattaaatt ttttaagtta
    40681 gataaaaata gaggtaaagg atatgcttta aattatggta tcaagttagc aatgaaaaat
    40741 gcagatatta taggatttct ggatggagat ttaggaagct ctgctaaaga agttgaaaaa
    40801 ttaatagtac ctatattaaa tggtgaggcg gatgttacaa tagccaaatt tcctcctgca
    40861 aagagaaaag gaggtttggg atttgtaaaa ggtttagcta aaaattctgt gtttgagatg
    40921 actggtgttg agttagatgc aactttatca ggtcaaagaa tatttaaaag agaagtttta
    40981 gaaaagtttg atgaaatacc ctttggatat ggagtagaag ttggaatgac cattgatata
    41041 ttaaagtatg gatttaaaat taaagaagta ttagtaaata tgactcataa tgaaacagga
    41101 agaaatttaa aaggttttat tcatagaggg aaacagtatt atcacataaa aaaggtttta
    41161 ggacaaaaaa agaaagaatg gaggtaatat cttagtgaaa gattatatac tttataccat
    41221 tttattatta actggattat ttggaaccta tgctgtaatt ccactattta aaaatttatt
    41281 gataaatgga aatgtactta gaccaaacta taaaaaagat atgataccag taagtatggg
    41341 aatcgtattt ttgcctatga ttgtaatcaa tggaataata cttgctttct ttactacaga
    41401 atttaaggat ttagcgtata tatttatgtt tatgtttggt atgatatcca tgttttttgc
    41461 agggatacta gatgatgtca ttggaaatag agatgtaagt ggtttaaagg ggcattttaa
    41521 aagtttattt aaaggaagtc ttacaacagg aggttttaaa gctttatttg gtggatttgt
    41581 agggcttata gtttctgttg ctatatcaaa agacataatt gatataatag taaatacact
    41641 aataatagct ctatcaacta atttaatgaa tcttttggat ttaagaccag gaagagcaat
    41701 aaaagcatat ttattcataa tggtaattat atttttaaca ttgacaggat ttgttcaagt
    41761 tcttccatta cttatagtac caaatgtact tgcatacttt aattatgatt taaaagcaag
    41821 ggctatgatg ggagatactg gttcaaacgt ccttggaata tctattggaa tgctcatttc
    41881 ttttgggtat acatttaaca ttagattagg atggttaata tttgtagtta ttatgcatat
    41941 attcactgag aaatactcat tgactaagat aatagaaaaa aataaactat taaattttat
    42001 agataaattg ggaaggtagt attatgataa gcaaaagagt ggggattgtt gaatcaatag
    42061 taagtcaaac tgaaactcta gatgatataa gagtaaatat aaatggagaa atacaaaggg
    42121 catataatta tccaaaaata tctggaacta taaatatagg tgatgaagtg gttttaaata
    42181 caacagcagt agaactgagc ttaggtacag gtggatacca ttttgtaata actaatttaa
    42241 ataatataga atctacttta actgaaggtg gccatataat gaaacttaga tatacacctc
    42301 ttcaaataaa agtggattct gttgaggaac aagaaagtat ttatcatgat aaatttgcga
    42361 attttaaagg attagaaggt ctttctgttg tagtagggac tcttcatagt atgttaactc
    42421 catttgcagc ttcatttaaa agaaataatc caaataaaaa gttagtatat ataatgactg
    42481 atggagcatc tcttccaatt tatttaagta aaaatgtgga tattttaaaa gaaaaaaaat
    42541 taatagatag tacgataact ataggaaatg cgtttggtgg agattatgaa tgtataaata
    42601 tatatactgc attaattaca gcaaaagaaa tactaaaagc agatgcagta tttgtaagta
    42661 tgggacctgg aattgctggg acaggaacaa aatatggatt tacagggatt gaacaaggct
    42721 ctattcttga tgctgtaaaa aaacttgaag gaagagcaat tgcaattcca agaattagtt
    42781 ttgctgacaa acgagaaaga catcaaggta tttcacatca tagtatgacc gtatttaaag
    42841 aaattgtaaa tgtaaatgta gacataccaa taactatata tgatgatgaa aaattgaact
    42901 ttataaaaga gcaaatcaat ttaaatggtt tagacaaaaa gcataatata atttatatag
    42961 ataataataa aacgaaagag gatttagact attttaatct aaaagtaaag agtatgggaa
    43021 gaaattatga acaagatgag gcatttttca aagctgcaag cacagcagca tattacttaa
    43081 tggaggttta agatggtatt agaagaaaaa actattagta gtgatagggt ttatacaggt
    43141 aaagtaatta ctttaaaggt tgatactgtt gaaattccag ggcaaggata tcaaaaaaga
    43201 gagttagtag aagttggtgg agcagtagga atagttgcaa tcacagatga taataaagtt
    43261 gtgctagtta agcaatttag aaaacctata gaaaagccaa tctttgaaat tccagctggt
    43321 aaactagaaa aaaatgaaag tccaaaagaa tgtgctgaaa gagaattaaa agaagaaact
    43381 gggtatagtg caaaaaatat taagctaata cataaatttt tcacttcagc aggtttttct
    43441 aatgagataa tgtttgtata tttagcaaca ggtcttactc caggagagaa taatttagat
    43501 gcagatgagt ttttagatgt ctatgaaatt gagcttgaag aagcatataa tatggtatta
    43561 aaaaatgatg ttgaagatgc aaaaacatct attggattat tattagtaaa agatatgttt
    43621 aaaaattaat atttataaaa tgaataaccc tttgtcctaa agcatattat tttataaagg
    43681 acaaggggga attaaattga gaagaacata tagaaaaaat gattttagag aattaaatag
    43741 acaaataatt attataggat tgttgtttat gatgtctatt gttgttgggt cttacataaa
    43801 taaaatattg ccaggttcta gtaacaatat attaaacaat ataaatcctg ctgtagagta
    43861 ttataatcta aatataagca taaaagatac agtgatacaa aatttaaaat cagatgcagt
    43921 ctttatgggc tcaatagctc tattaagtct atttgtagta acaattccag cagtgttggt
    43981 agcttttgtt ttaaaaggga tgtcaattgg ttatacaata aatagttgta tattggcatt
    44041 aaaatttaaa agtataaaaa tgattttaat aatattattt aaaaatttga ttattattcc
    44101 atgtgcaatc atattagcgt taattagttt aagttatttt aaagaaatgg tttatgaatt
    44161 taaaaagaaa aatagaaaaa atatgcaatt tttaattaag aggtatatac taaatattat
    44221 aattataata gcactatcat tggggttgca attaatatta aatactgcaa gtattggtat
    44281 tattaagttc ctagctaaat aaaggagttt ataatggata ttatagaggg atatatagat
    44341 tacataaaag ataaaaaaaa attatcagag aatacagtgg catcatactt tatggatata
    44401 aaaaaatata tggaatatat aaatcaaaaa agcattaaac tagtagatat agtagaaaat
    44461 gatattataa gttacttaat aaagttggaa aaagataatg tatcaatagc tacaatagcg
    44521 aggatgatat cctctataaa atcttttcat gattatttat ttttaaatca tatatgtaca
    44581 aataatcctg caaaggatat aaaaaaacct aaaataaaaa aagaaaatat aaatatactt
    44641 acagaagaag aaattgaaag actattaaac tttccaaagt taacaacacc taaattgatt
    44701 agggacaagg ctatttttga agtattatat ggtactggta taaaagtatc tgaattagta
    44761 gagatgaata tagaggatat tgacctagat attgattata tatattgtaa ttcatgttgt
    44821 aaaaatcaaa gagtgatacc actttgtgat attacaaaac tttacttaga aaagtatcta
    44881 aaagaagcaa gacctaaaat ggctgtagaa ggtgaaaaat cattatttgt aagctcatta
    44941 ggtcaaaggt ttacacgaca aggtctttgg aaagtcataa aaaaatattc taatttagct
    45001 aatatagata aaaatataaa tcctacaatg cttagacatt catttgcaat tcatttgtta
    45061 aatgaaggtg ctaacattgc tgttgtaagt aaaattttag gaaatgttaa tttatctagt
    45121 ttgcaagtat atttaaatca tatagataaa aacgtgagaa gagaaataaa ggaaaaacat
    45181 cctagaaatg atgttgatgt agaattacaa aaagcagaag ctaaatagac tatatagcaa
    45241 attttataat ttagaaggag catatttgta tgagcagagt aatttggatt gtaattgata
    45301 gtgttgggat aggagcatta cctgatgctg aaaattttgg agatagtaag gatgtaagta
    45361 ctcttggaaa tatatttaag aaacatccag atatccagat tcctaatatg agaaaattgg
    45421 ggataggaaa tatagatggt gtagattttt ttgaaagtat aaaagaacca ataggttgtt
    45481 ttggaaaatg caaggaaatg tctcagggaa aagatactac tacaggtcat tgggaaatga
    45541 ctggtataat agttgacaaa cccttcaaga catttgaaga tggattctcc aaagagataa
    45601 tagaagaatt tgagaaaaaa actggaagaa aagtagtagg aaataaacca gcttcaggaa
    45661 ctgtaataat agatgagtat ggagagcatc aaatagaaac tggagatgta atagtatata
    45721 catcagctga tagtgtattt caaatagctg ctaatgaaga agtaattccc ctagaagagc
    45781 tctataatat gtgtaaaatc gctagagaaa taatgatggg ggataatgca gtagcaagag
    45841 taattgctag accattcata ggtaagaaaa aaggtgagtt tgtgagaact tcaaatagaa
    45901 gagattactc attagaccca tttgaaccaa cagttcttga taatataaaa gaatcaggac
    45961 ttgatgtttt agcagttgga aaaatagaag atatttttaa tggaaaagga attacagatg
    46021 ctatccacac taaaagtaat atggatggag tagatgaaac tttaaattat atgaaacaag
    46081 ataataaagg tctgatatat tcaaatcttg ttgattttga ttctaaatat ggtcatagaa
    46141 gagacccaga gggatacaag aaagcacttg aagagtttga tagtagactt cctgaaataa
    46201 tggctaatat gagaaaagat gatattttaa taatcaatgc agaccatgga aatgacccaa
    46261 catataaagg tacagaccat acaagagaat atataccagt tatgatttat ggcaataaga
    46321 ttaaaaaagg ttttaattta ggagtaaaag atacttttgc tgatattgga gcaactgttg
    46381 cagatatact gaatgtaaag ttgcctaaac atgggtcaag ctttaaagaa gatttatttt
    46441 aaattagaaa atgataaaaa cttaggttaa taggatggag agattggaat gtttgaaaag
    46501 atagcacaaa gtagtgaatt tataaattct aaaattaatg taaaacctaa aattggtttg
    46561 atattaggtt ctggactagg ggatttagca aatgatatag aagactctgt cacaattaaa
    46621 tatagtgaaa ttccaaactt ccctgtatca acagtagcag gacatgctgg acaacttgtc
    46681 attggaaagc ttgaaggaaa agaagttatt gctatgcaag gtagatttca ttattatgaa
    46741 ggctattctc aaaaagaagc tacttttcca gtaagagtta tgaaagaatt aggagttgag
    46801 attcttatag taactaacgc tgctggaggc gtaaataaag aatttaaagc aggagatttg
    46861 atgcttataa gagaccatat aaactttagt ggaagtaatc cattagtagg aaaaaatgat
    46921 gatagatttg gtgctagatt tccagatatg tcggatgcat attcttctaa atatgtagat
    46981 gtagttaaaa actgcgctaa agaatgtaat atagatgtca aagaaggcgt ttatatgttt
    47041 tttagtggtc caaactatga aacaccagca gaagtgagaa tggcacaaat tttgggagct
    47101 gatgctgtag gtatgtctac tgttccagag gtcatagtag cttctcactc taatatagga
    47161 gttattggta tatcctgtat aacaaatatg gctgctggca tattaaatca gccgctaagt
    47221 catgaggaag taatagaaac tactcaaaaa gttaaatcag aatttatgaa tttagttaaa
    47281 acaacagtta aaaatttata agttaaagat tattatagat tttttgagca agtttataat
    47341 aaaatagtta atgtttaatt tgaaaagaat taataaagag ctgtcttgat atagaaagtt
    47401 atttctaata aaggacagtt tttttataat actacaacat ttgaaagtgt tttaaagttt
    47461 aatatttatt taaattatta taggttagaa tggagatttc aattatgaga atgtatgata
    47521 ttataaaaaa aaagagagat aacctagagc ttagcaaaaa agagataaac tactttatag
    47581 aaaactattc aaagggagat atacctgact atcaagcttc tgcactatta atgtcgattt
    47641 atttaaataa gatgaataaa caggaaactg tatatcttac agaagcaatg atgaactctg
    47701 gtgacatgat aaatctatct gagatagatg ggataaaggt ggataagcat agtactggtg
    47761 gtgttggaga taagactact atagctctta tacctttggt tgcttcatgt ggagcaccag
    47821 ttgcaaagat gtcaggcaga ggtcttggtc atactggtgg tactatcgat aaactggagt
    47881 ctataccagg tttttcggca gaaatggaga taagtaagtt tattgactcc gtaaataagt
    47941 ccaaaatagc agtttgtggt cagagtgcaa aagtagcaat tgcagataag aaaatatatg
    48001 ctcttagaga tgttacagca actgttgata atatatcatt gatagcatca agtgtaatgt
    48061 ctaaaaagtt agcttcagga gctgatgcaa ttgtgctaga tgtaaaaact ggtgatggag
    48121 catttatgaa gacattagaa gagtcttttg agttagctaa atccatggtt gatataggta
    48181 ctggtatgaa tagagataca atagggattg taacagatat ggatgaacct ttaggttttg
    48241 cagttggaaa ctctttagaa gttatagaag caattgagac attaaaagga aatggaccta
    48301 aagaccttgt tgagctatgt gaagtattag gagcatatat gttggtttta tcaaaggtag
    48361 cgcatgattt tgaagatggt gttgaaaaga taagagaatc aataaaatct ggtactgcta
    48421 tacaaaaact caaagagttt atagagaatc aaggtggtaa taaagatgta gtagatgatt
    48481 attctttatt caaaaaatca aagtataaat atccagttaa atcaactaaa tcaggttata
    48541 taagtaaaat taaagcggaa gatgttggtg tatcagctat gattcttgga gcaggtagag
    48601 aaactaaaga tgatatttta gatttatcag cagggataat tttagaaaag aaagtaggag
    48661 attatgtaaa tgaaggtgaa gttttagctt acatgtatta taatgatgaa caaaagcttt
    48721 cattggcaaa gagaagattt attgattctt attctattgt agattcaaaa atagaaaaaa
    48781 ataaattaat atatggtatt gtaactaaag aagatattaa aaagttttag aaaattttag
    48841 aaaagtgatt ataaaaagcc taacatgtca aaaataatgt taggtttttt attttttgat
    48901 aagatataaa tgatggcaaa aatatatatt ttaaaacatt ttttacaata taaataagtt
    48961 tattctacaa gatgattgaa aattaaagta tctagtttta taatagaaaa tggataaata
    49021 tacataagga gtagattata ttatgaactt taaagaaaat agattgaaaa tagcagtatt
    49081 aataattgtt attttgatta tcttagcagg tatatttgtg tttatacaaa taggtcctta
    49141 tgataaaaat aacaaaaaag atgtaattat tgacgttcca agtggagcat ctgttggaaa
    49201 aatatctgac atattatatg aaaataaatt aataaagaat gaactattat ttaaactttt
    49261 ggtaaaggtt agcaataaag ctccatctat aaaatcagga acatacttac ttaatcaatc
    49321 gtattcaaat aatgatataa taagtctttt agtttcaggt aaaatatatc aggatggtat
    49381 aaaagtgact attccagagg gagctacatc taaagaaatt atagcaatgt tagtaagtaa
    49441 aaatttagga gataaagcta cttttgaaaa tttaataaaa aaacctcagg aattttatga
    49501 taagtttcca tacttgaagg aagatggaat aacctcttta gaagggttct tgtatccaga
    49561 aacttattac tttaattcaa agaaacaatc agaagaagat attttaagcg aaatgttaaa
    49621 agtatttgac agtaaataca cggacaagtt taaaaagaaa caaaaagaat taaatatgac
    49681 tcttcaagaa gtaatggaaa tggcatcaat aattgagaaa gaagctgttc ttgacaaaga
    49741 cagacctata atagcaagtg tgttttataa tagacttaag gtaggtatgc ctcttcaatc
    49801 agatgctaca atacaatata tatttgaaga gagaaagaaa attgtaacat atgatgattt
    49861 aaaaattgat tcaccatata atagttataa aaataaggga ttacctccta caccaatatc
    49921 aaatcctggt atagaatcta ttgaagcagc attatatcca gataaaacag actatttata
    49981 ttttgtagct aagattgatg gtggaaataa ttatagtaca aactatcaag accatttaaa
    50041 atatgttaaa gaatataaag aagcaaggga taaacaatca aaagatacaa aagcaacaaa
    50101 taaagaaaat acaaaaagat aaaccatgat tataaaaagt ctaacagggt aagaataatg
    50161 ttgggctttt ttattgaaaa ttgttattct tagatttata ataaagaaag tgtaaatata
    50221 tataacaata tgaatttaaa aaataataaa ttaatataaa acttgaatat ttattatttt
    50281 agggagtgtt tttatgagta atatagttaa tgatttggtt gaagattata taagagtgac
    50341 attaaaagaa aaagaaggat ttttaaaaga tttagaaatt tatgcagagg aaaatagtgt
    50401 tcctataata cataaagaag tttcagattt attaaaagtc ttattaaagg ttcaaaaacc
    50461 aaagagaatt ttagaagtag gatgtgcaat tggttattct tctatttttt ttgcaactat
    50521 tataggaaaa gatgcggata taataactgt tgagagaaat gaaaaaatga tagaaaaagc
    50581 aaaagaaaac ataaaattag ctggatttga caataacata actatactag aaggagatgc
    50641 agaagaaaaa cttagtcaag tccaaggtga atttgacctt atatttatag atgcagccaa
    50701 aggtcaatat aagctatttt ttgatttagt tatagataaa ttaaaagatg ggggactttt
    50761 agtatctgat aatattttgt ataaaggtat ggttgctcat gatgattttg ttgtaagaag
    50821 aaaaaagact atagttaaaa gaatgagaaa ttatttagat tatatatgta attgtgacta
    50881 tttaagcaca tcattggtac caataggtga tggtgtagca ctaagttata aagaaagaaa
    50941 aagaggtgac gtagatggaa ttaaataaag tagaactttt agctccagca ggtgatttgg
    51001 agaggttgaa aatagctata acatatggag cagatgccgt ttatataggt ggagaaattt
    51061 ttggaatgag gtctgcagcg aagaatttta gtaaggaaga tatggcagag ggagtagctt
    51121 ttgcacatga aagaggcaag aaagtattcg taacagtaaa tataatacct cataatgaag
    51181 attttctaca attagaagac tatttgttgg aactagaaga tataggaata gatgcagtta
    51241 taatagctga cccaggagtg cttagtgtca ttaaaaaagt aattccgaat atggaaatac
    51301 atttaagtac acaagcaaat acaaccaatt atctaagtgc aaacttctgg tacgagcatg
    51361 gaataaaaag agttgtagtg gcaagagaat tatcttttga tgaaatatct gagataagag
    51421 caaaaacacc tttagatatg gatatagaag cgtttatgca tggtgctatg tgtatttctt
    51481 attctggtag atgtttaata agtaactata tgactggaag agatgcaaac aaaggttctt
    51541 gtgctcaatc ttgtagatgg caatatcatt tggtggaaga aaaaagacct ggtgagtatt
    51601 ttccaatata cgaagatgaa aggggtacat ttttctttaa ctcaaaagat ttatgtatga
    51661 tagagtatat accagagtta atcaaatctg gcataacaag tttaaaaata gaaggtagaa
    51721 tgaagactgc atactatgta gctactgttg taagagcata cagaatggca atagatgaat
    51781 tttacagaga cccagaaaac tggaagttca atccaatgtg gatggaagaa ctaaaaaaag
    51841 gtagtcatag acatttcaca tcaggttttt acttgaataa accaacgaca gaagaccaaa
    51901 attatcaatc agcatcatat gttagaaact atgattttat aggtatagtt agagaaacag
    51961 aagatgaaga tggtcttatt gttgttgagc aaagaaataa aatgtgtgtt ggtgatgaaa
    52021 tagaagttat gggaccatat aaagaaacta tgtttactaa aattgaagaa atgtacaatg
    52081 aagaagggga agctatagaa tcagcccctc atcctagaca aatagtgaag ttaaagctat
    52141 cagtaaaagt tggtaaagat tatatgctta gaaaagttat agaagaaaag gttgaagaat
    52201 aataaaaaat tgataaaaat agtattagcc ccttagaaat tgtcaataat gaaattatct
    52261 aaggggtgat ttcatgtcaa aaaagaagac tccatttttg aaaaaagtgg gaaagagaag
    52321 ctggtgtata tttactataa ttttaataat ttatagtgct ttaatttata ggctagttga
    52381 tatacaagta ttaaaaggtg ataaatacaa acaaagtgtt gaatcacaaa gtgttgagaa
    52441 agtcgagtta aatagtggaa gaggaataat ctatgataga aacaacaaga agcttacaga
    52501 tacaagtaaa tctcaggtac tggttgttga aaaagaaaaa ttaaataata gctataaaat
    52561 tttggagctt attaagaaag ctactaagat gaatgattta gatatatata aagctgtaca
    52621 agagcaattg acacgcccta taatacagat tcagactaaa aatatcgata agagtatgaa
    52681 aaaagaactt gagaaaaatg gaataatggt agaagaaaaa actatgaggt acgcaaaaga
    52741 tggtttatta tcacacacaa taggatatat aaaagaagat gataaatcag gtcaatcagg
    52801 aattgaaaag agtatggata gtgtacttag aaattcaaat gaaaagtata taagtgcttt
    52861 taaagctgga gatgcaggaa atgaaaaatc tctaaacata ctaaagggaa gtgtaaaaac
    52921 tgttgataat aaggataaag atagacatct aaaaactacc attgattata acattcaaaa
    52981 aaaattagaa caaatactaa ataaagaaga aaatcctaca gcagcaataa tctcggaagt
    53041 atctacaggt gaaattcttg caatgtgctc tagacctaat tttgaccaaa atgacatatc
    53101 aaaaagttta aaaggaaaaa atggtgaatt tgaaaataga gttataaagg cgacatatcc
    53161 accagggtct gtatttaaga tggtagtttt attttctgca cttgaaaatg gtgttataga
    53221 tgagaactat acttacaact gtacaggtaa gactaaagtt gggaatacta atgaaatact
    53281 gcgatgtaat aaaagagatg gtcatggttt ccaaaactta agacaagctt tttctaattc
    53341 atgcaatcct gcatttttag atatagctat gaaacttgga aaagaaaaga tactcaaatc
    53401 tgctgaaaaa ttacatttat ttgaaaaggt tgatattggt cttgatgaag aaaaaattag
    53461 agaagctcca aaaaacatat caataagaaa tttagctata ggtcaagaaa atatagaatt
    53521 tacaccactt caaataaatc aaatgactca aataatagct aataatggaa cattcaaacc
    53581 actatactta tataaaagtc ttgtggacaa tgatatgaat acaataaaaa cttataagtc
    53641 ttcgaaaaaa gaagatctta tttctccata tgtgtgtaca caagttaaag aatatatgaa
    53701 gtctgtatct aggattggta cagctaaaga cctaaaagat attgagggtg gttgtggagt
    53761 taaaactggt acagctcaaa gtagtttaaa taaaaaagca atagaccatg gatggataac
    53821 tggattttat cctgaagaaa gacctaagta tgtaataact gtattagtag aaggaactca
    53881 aaaaggaaat aaatctgcaa cacctatatt taaggaaata tgtgaaagta taaaataaat
    53941 tatgtactat aaaatttaag gatatatcgt aactttgatt ttagatagaa aattttgtct
    54001 aatgatgtac gatatatcct tttaaaatat agtattaact ttcttgaaca agctcttttt
    54061 ctagtttttt aagagctttt ttttcgattc tagatacata tgaccttgat atatcgagtt
    54121 tttgtgcaat ttctctttgt gtcttatagc cataaggagt taacccatat cttaactgga
    54181 caatttctct ttctctagga gtaagaattt tgtttagttg ttcgtaaagt tttccaactt
    54241 gaacttttag ctcaacttca tcaagaacat agttagcctc tgtgccaagt atatctaaaa
    54301 gacttatttc attacacact actctttata tgtcattggt attactgttc ttcatgtaat
    54361 ttcaaatgga ccattaattc aaaagaatct agtttttgct ctttagactt ataataaact
    54421 gccttatcaa ttacagattt taggagttta ttttttccaa gggaatctga atcatggtaa
    54481 agtgataaca cagttttaat atcttctatt attttagaat tgtctttttt aacagtactc
    54541 aatctatttt ttactgtctt aattgaatct ttaatttctt cagttcttga acttatattg
    54601 ttggaccttt caataaatgt atcttcatca tatattcctc gttctagtaa atcatataac
    54661 ttattttttt gcttgcctaa ttcttttaat tcagtttcta aaccctttaa gactcgtttt
    54721 tgaaattcta tattactatc tttattattt ttatctaaat ctaaatcaga tgattttaat
    54781 tgatattttt ttacagtgtc ttccaaagat tttaaaatgg cagcttcaat aaattcaaat
    54841 cgagaacttt tattgcaacc aggatgataa caaattatat agtcatggtc tgcatatggt
    54901 ctatatacta atggtctacc acaatttttg caaatcacta ttcctgcaag tggatttttt
    54961 atttgattac catagctgac atgaccattt tttttgagta tattttgagc ctttttccat
    55021 gttatttcac ttattaaagg ttcatgcttt ccttttgcct caatccattc atctttaggt
    55081 cttagtttaa ctgtttttat tttatttgga tttttagatt ttttgtagtc ctttttttgc
    55141 cactgaatgt agccacagta gaccttattt tttattatat ttgtaattgc agaattgtac
    55201 caattagcac ctgtagcagt tttaatgcct aagttattta ggtattttga tattttacta
    55261 caacccatat cttcattaat atataagtca aagatagtcc taactacatc agcatcctta
    55321 tttggtacaa gtattctttc ttttctacct gttgttacag catcatatcc aaatggagca
    55381 gaagttccta tgaaattacc ttcttcaact gattttattc tacctctttg cattcttctt
    55441 gatataagtt ttaattcttt tcttgccata aaggcttcaa attctgagta ctcctcatca
    55501 aattcatctg ttaggtcgta tgtttttcta ggtgtgatga ttttggtctt actttctttg
    55561 aatgtttcta atatgatacc ttggtctttc atgtctccac gaccaagtct gtctaagtcc
    55621 attactaaga ctgcatcata tttattttct tcaacttctt ttagaagttc aagcattttg
    55681 ggtctatgta ttatactttc accagaaact aattcttctt ttatttcaat tacatctaag
    55741 ttttgttctt ttgctagttt tagtagggtt gatttatgtc tacttaaagt ttcaaattca
    55801 ccttgctttt ctgcatcttc atcagcacgg gattttctta agtatatggc aacactcata
    55861 ataagtaatc acactcctta ggtactatta tatagtaaat caatagagtt taaaatatag
    55921 aaaataagca tgtcaaaagt taatttaact ttcaacatac ttattttcta taatattaat
    55981 gccttataat cctttaatat catttagatt agtttttgca tattcattat ctgggttaag
    56041 ttcaagcgcc ttattgtaag attctctagc tttattaaat tctgatttac tcataaaaat
    56101 aacacctctt aaattgtaag catcactagc gtctatttta taagcttcat tatttttatc
    56161 tagatattct aaggatttat caattgactt taaagcctca tcatatcgtt gtaactctat
    56221 ataaatatat gattgtgtat aatatgcagg tggattttta tcatcaattt ctatagcttt
    56281 tttgatatca atagcagctt tattataatc attatcttca tctttttttg tttctgacct
    56341 agcgattttg acataattag agctaagagc tcttttaaag tctttatgcc ataataattt
    56401 atattcatta tcttcgatta taaaatatct atcaaaattt gcagtatctt ctttattttc
    56461 agtgatatat tttatagtat cagtttcttt tattttaaca agatctgtat actctttatt
    56521 agaatatttg tatttttcta ctttattttc ttcaccaagt ttataatctt ttagggtttg
    56581 aagtttaaat aaagtatctt gatattcttc aaatttttct agactgacac tttccttact
    56641 gtctttactt aataaattat atgctacttc gtagtctttg atttctatac tatcaaggta
    56701 ctttttagct acctctgtag cactttcttt tggaacacat ccaaccattg atatcataat
    56761 taaagatgat actaatattg ctgttatttt tttactgatt atcataatat aatccccctt
    56821 atattgtctc tcactaattt tattttatca ccatattcta ccaaagtcat taatttaaga
    56881 atgatatttt tcattaatat tatattaagt aataataatt tttaaaattt atggtaacat
    56941 tttcctgaaa agtgttatat aatttaggtg aaaatattta agagaaggag atattatgaa
    57001 aattaaaaaa ttattaacaa tagggttatc tttatctatg tttattgcta gttgccctat
    57061 atctgcaaat gctcttgata aaattgaatc aattaaaggt gcagataaat acgaaactgc
    57121 tggtataatt gcagacaaac aaaactatac tacagctata ttaattaatg cagacagtac
    57181 aatggctgat ggattaagtg caagtggact tgcaggagct ataaacgctc caattctttt
    57241 gactaagaaa aataacatac caaatgctac acttaaaaga ttagaaaagg caaaaaaagt
    57301 atatataatt ggaggagaaa actctattga taaatatact gaaactgttc taaaaggcaa
    57361 aggaatagag attaaaagac ttcaaggtag tgatagaatt aagacaagct ataatgtcgc
    57421 aaaagaaata aactcaatta acaaagtaaa taaagtcata ttgactaatg cttttaaagg
    57481 agagcctgat gcaatgagtg ctgcacctgt agcagttaga gataaagcag ctatagtatt
    57541 aactgatgga aaaagtgttc catttaatac aactggtgta gaaagttatg taataggtgg
    57601 tacttcatca atgaatgaca aactagttaa ggatactaat tcaactagat taggtgggac
    57661 agatagatat gatactaaca aaaagataat aaataaattt tatagtggag taaaagaatt
    57721 ttatatagca agtggtactg atttagtgta tgctcttgtt ggttctaccg ttgctaaaaa
    57781 taatgctatt gtgttagttg ataatgatag taataaaagt gtattgaaaa gtacaactaa
    57841 attaactgct ataggaaatt tgagtgatag tatattacag cagtgtttaa atgtaacaaa
    57901 aaatataggc gattctaata ctggaagaga atatacagta gaagatgctg ctaatgcagt
    57961 gaaaaaacta ttaggtgtga agaaatttta ttacgaacct aattattcag aagatgaaga
    58021 tttaatattt ctactagata aaaattttga tttttcagga aaagagtact atgtatttgc
    58081 ttacattaaa cagggtgcag agggtgatgt tagatactgt gtagctaaaa atgatatgag
    58141 caaagtttat acatatgatg ttaatggtaa tatgaaagta tgtaatccag atgattataa
    58201 aaataaagga agtataacag aggaacaagc aaaacagata gcattaaaag aatgtgctaa
    58261 gagacataaa gtcagtatca ataacttagt agttgatatg atagatttta gagatgatat
    58321 atatagtggt aatgtctatg ctgttaaaat caattcaaaa aatagtaatg gtacttttgg
    58381 ttggtttttt atagatatag aaactgggaa aatttataat taaaattaga acttttaaac
    58441 aataattata taatttacca taatatgtta taatattttt agcaagaaga tttaacttac
    58501 aatctataga gtggagttca tgtacaaaag attatcctcc caacgattcg aagggaggtg
    58561 agttcatgga taactttttg tttaatgttt tagctagttt aacagctagt gtggtagttt
    58621 acttaatcag taaactattc aaaaaagcaa aaagccactc tcgcacaaag agtgacttac
    58681 gagttgaatt taaatttata tttaaatcca aaaaataaac tctgtttatg atgaactcca
    58741 ctctaacgca aaatagattg tagttcttct tgcttttatt ataccacaaa atattataaa
    58801 taaaactgtt gtttataaaa aaatgcaaag taaaattttt tataaacaat gataaaaatt
    58861 ttattataga gctattaact tatgaaatat ctgtaaatga gcagatattt cataagttaa
    58921 tatttattgt ttacataaaa tatttggtaa aaataagtaa gttttatatg ttataatagt
    58981 tgtagcaaga ataataatct aaagtggcaa gcatgtcagc tggtgttata tcagtttata
    59041 tttatgataa aataaaaatc tcccaaacgc caataagaat gatttaaaaa aataatattt
    59101 tcacttaaaa ataatcactc tttataggag taaattattt tcttgatttt attatactac
    59161 aaattggtac agatatttaa aaataatata ttcataatat tataaatagt tttgcagtga
    59221 gcgatatttt tgataaaata gggcttaaca gttgaaacat aaggcattga gggtatatga
    59281 taaatattat catttgcact actcgtggtt cactgcaaat ttgagagaat tgtatagatg
    59341 taagtgttgg aagtactcaa tttattttgg ggttttatat taactaagtg gtatgtaaag
    59401 actgaaccta ctaacattgt agggttgaag tctacaagtt ttatattaac taagtggtat
    59461 gtaaagacag atataatatc aaaataaggt taagactttt aaaagcatag ccatatcagt
    59521 aaaaatttaa ttatcattta aaaataaatt tttatttaaa gaatacacat aaatatttcc
    59581 agactcaact ctcttaaact attttcatat cttttagtag atacctttgt tgttttttag
    59641 tctttagcaa cttgttattg agtgaacctc ttattttttc taagttattt caagcttttc
    59701 aaaaaattct ctatttatat tcatcacaca tataatacaa ttaattcagt tttaattgcc
    59761 aatatttagt ttttctgtat ctgataaacc aagaatataa tcagtatata aaccaaaaat
    59821 tttagcaaat attattaacc catcgtctct tgttggtctt tcaccagact ctattctatt
    59881 cataatactt gtatttatat ttgttttttc aagcaattct ttttgagaac tattcatatt
    59941 ttccctaata tatttaatcc tttgatttac atccatacat aattatctcg aaaagtttct
    60001 gctttggaaa tattgttatt gaaatataga aattaatgtg aatatatgaa aaagatacac
    60061 tatgatttta taaaaaagaa gacatacata aaatttatac ttacaaagtg ttgcaaaaga
    60121 gttaggtttt aaaaatgcat caacttattt taagtacagg aaagggtaat attcaattag
    60181 agttgatata ttacctataa actatctaag atacttgatt atgatataga aaatttaatt
    60241 aagaggatga taaggatgaa taatttacaa gtaatataaa gaaataataa gagaatttta
    60301 aatacatagc aactagcaga tatatatgaa aatgatgtaa acaatatata aactaacttc
    60361 aatagaaata aagagaatgt aaattatgtt ttattacaat tcgagtattt gaaagaattt
    60421 aagaactaat ctattaatag tcagttgatt tcaaaacatt ctagtcaatt atacctttgg
    60481 accgaaagat gttcaaatat acactacaaa gtattagata ctgataaaac ttgagaacaa
    60541 tctgacaatt tagagaaaac atatttcaag gttaagcgag agaaactaac ctgtatagaa
    60601 gatgtattaa tagaaaattt aagataaatt gaagatttaa gaatacaaat taatcaagca
    60661 ataatatgac attagaggta aaaataaaag ttaaaataat aaaagatgtt gttatattaa
    60721 atataacaca ttggaaaaaa gaaacaagtc tattaacagt taaaatagct agaagtcaag
    60781 gtgatttaag ttatgtaaaa tattttgcta atgagagtta agagcttctt aacaagagtt
    60841 ttggtgtgtc tcttgaaaca aggttaacaa ttaacaaaag acgaatggca gaagaaggag
    60901 tatgtaagtt taaaagagat aagttaaaca aactagatat tatataagag gataagaaac
    60961 ttatagaaga atatgttact tttgtaaagg atttagcttt aaaatataga atataaagtg
    61021 attttaagta aaaattgtaa caattaaata agtataagac aagtttatga gttcatattt
    61081 tataacaagg ggtgatgtta tgaaaatact acaacaattt aaaccataca aatatgataa
    61141 agaaaaaaat atagaatatg atgaaaaaat tatagctaat caaaacacag aagtttatat
    61201 agtatatcca cgtatatctg atgaagaaaa taggagaaga tgggaagagt tcaatgaagt
    61261 agctaaagaa gtagctatta atatagctat gaagaaagtt gagacagtag aaagttaaga
    61321 gaatagagaa gttgctaata cttagcactt tctcatagaa gaaaattgga caactatttt
    61381 taaataatta ggaataaaaa agttaaatca tatttgaaaa aaggcgataa aattgttgag
    61441 tattgttatt agtaataaga atgtattaac tatgtattaa aagaaattat aagtttttag
    61501 taaatatagt atttaaggat attaaaactg gtaaaaaata tcatttagac tattaaataa
    61561 aaaagtgctg gtaaaaataa gcaacacaat aaataaaaaa acttatagaa attataagat
    61621 aaaaagatga gatttgaaat ggttgaagtt aatataaaaa ttaaaggtga agtaagtgaa
    61681 ataattgact c
//
LOCUS       DAEMAE010000003        59279 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEMAE010000003 DAEMAE010000000
VERSION     DAEMAE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.003"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..831)
                     /locus_tag="KPH93_000125"
     CDS             complement(<1..831)
                     /locus_tag="KPH93_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG1686973.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NYSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PRTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDTDLE
                     D"
     gene            complement(885..1235)
                     /locus_tag="KPH93_000126"
     CDS             complement(885..1235)
                     /locus_tag="KPH93_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG1686974.1"
                     /translation="MINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKLNAD
                     YTNAANIVTAASMAINDDEKIIDSLSVETLKEKGYLNTVPVPQSTSGKFELVINDNGT
                     DISVNINSKQFYPK"
     gene            complement(1289..2374)
                     /locus_tag="KPH93_000127"
     CDS             complement(1289..2374)
                     /locus_tag="KPH93_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG1686975.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGTFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVYTSNG
                     IEPPLITRALIGISTIVTNNLAYLIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKRINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITSF"
     gene            complement(2545..3924)
                     /locus_tag="KPH93_000128"
     CDS             complement(2545..3924)
                     /locus_tag="KPH93_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG1686976.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSDEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLRGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3917..4597)
                     /locus_tag="KPH93_000129"
     CDS             complement(3917..4597)
                     /locus_tag="KPH93_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG1686977.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4989..6068)
                     /locus_tag="KPH93_000130"
     CDS             complement(4989..6068)
                     /locus_tag="KPH93_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1686978.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFYLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVNKVY
                     YLAGEIGTGKTTLLNKVYKKAVEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6364..6479
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6654..8024
                     /locus_tag="KPH93_000131"
     CDS             6654..8024
                     /locus_tag="KPH93_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1686979.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNENGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNVIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLAYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8289..8900)
                     /locus_tag="KPH93_000132"
     CDS             complement(8289..8900)
                     /locus_tag="KPH93_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG1686980.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8890..11253)
                     /gene="lon"
                     /locus_tag="KPH93_000133"
     CDS             complement(8890..11253)
                     /gene="lon"
                     /locus_tag="KPH93_000133"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG1686981.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLEAYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPDFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11433..11945
                     /locus_tag="KPH93_000134"
     CDS             11433..11945
                     /locus_tag="KPH93_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1686982.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11933..12460
                     /locus_tag="KPH93_000135"
     CDS             11933..12460
                     /locus_tag="KPH93_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1686983.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12531..13781)
                     /gene="clpX"
                     /locus_tag="KPH93_000136"
     CDS             complement(12531..13781)
                     /gene="clpX"
                     /locus_tag="KPH93_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG1686984.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13800..14384)
                     /gene="clpP"
                     /locus_tag="KPH93_000137"
     CDS             complement(13800..14384)
                     /gene="clpP"
                     /locus_tag="KPH93_000137"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG1686985.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14534..15820)
                     /locus_tag="KPH93_000138"
     CDS             complement(14534..15820)
                     /locus_tag="KPH93_000138"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG1686986.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(16014..16484)
                     /locus_tag="KPH93_000139"
     CDS             complement(16014..16484)
                     /locus_tag="KPH93_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1686987.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEIDAD
                     IVIFGHTHTPFREVKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDNINIEEIRI"
     gene            complement(16484..17833)
                     /gene="rph"
                     /locus_tag="KPH93_000140"
     CDS             complement(16484..17833)
                     /gene="rph"
                     /locus_tag="KPH93_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG1686988.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFIAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17993..20017)
                     /gene="ligA"
                     /locus_tag="KPH93_000141"
     CDS             complement(17993..20017)
                     /gene="ligA"
                     /locus_tag="KPH93_000141"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG1686989.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGIVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLKSHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKILDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVKFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20203..21141)
                     /locus_tag="KPH93_000142"
     CDS             complement(20203..21141)
                     /locus_tag="KPH93_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG1686990.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKDEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGLE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21243..21665)
                     /locus_tag="KPH93_000143"
     CDS             complement(21243..21665)
                     /locus_tag="KPH93_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG1686991.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMIVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21853..23136
                     /locus_tag="KPH93_000144"
     CDS             21853..23136
                     /locus_tag="KPH93_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG1686992.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSAFAFIFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23214..23729)
                     /locus_tag="KPH93_000145"
     CDS             complement(23214..23729)
                     /locus_tag="KPH93_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1686993.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23729..25105)
                     /locus_tag="KPH93_000146"
     CDS             complement(23729..25105)
                     /locus_tag="KPH93_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG1686994.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVTCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYSVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25130..25681)
                     /locus_tag="KPH93_000147"
     CDS             complement(25130..25681)
                     /locus_tag="KPH93_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1686995.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSINEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25718..25891)
                     /locus_tag="KPH93_000148"
     CDS             complement(25718..25891)
                     /locus_tag="KPH93_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686996.1"
                     /translation="MCTKKEEQKYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESN"
     gene            complement(25902..26735)
                     /gene="fdhD"
                     /locus_tag="KPH93_000149"
     CDS             complement(25902..26735)
                     /gene="fdhD"
                     /locus_tag="KPH93_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG1686997.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVESNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26784..27233)
                     /locus_tag="KPH93_000150"
     CDS             complement(26784..27233)
                     /locus_tag="KPH93_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1686998.1"
                     /translation="MEVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQ
                     INSNDAEKLGIEDDELVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIA
                     NLDPISKTPEYKYCAVKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            27539..30583
                     /locus_tag="KPH93_000151"
     CDS             27539..30583
                     /locus_tag="KPH93_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG1686999.1"
                     /translation="MKKTLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGKTAYCIDINTDFRNGYKTRADASSRMNANQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARTFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNASITDGNG
                     NYSIAGATYGVFTDKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETKKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSYGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTIEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDDDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDMKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDNNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYVIEELRSESNKGFELIPPFEIVVSRN
                     NLLIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRAENDYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATNKDGNKELEAGKNVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKCVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPQIPEKPS
                     RPSDSPKTGDSTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     gene            30816..31142
                     /locus_tag="KPH93_000152"
     CDS             30816..31142
                     /locus_tag="KPH93_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG1687000.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            31158..31541
                     /locus_tag="KPH93_000153"
     CDS             31158..31541
                     /locus_tag="KPH93_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG1687001.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFMQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHVPQGQTKK"
     gene            31709..32116
                     /locus_tag="KPH93_000154"
     CDS             31709..32116
                     /locus_tag="KPH93_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687002.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     INVIELSNLYSISLDELLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLIAL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            32132..32458
                     /locus_tag="KPH93_000155"
     CDS             32132..32458
                     /locus_tag="KPH93_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687003.1"
                     /translation="MNLNIVLAVICGAVALVGAFCVVFQIYHMTVIDATARGLKHPKF
                     WGVFTMSGNNSSGLLMYLIGRRKYPIVNMSESNSKELEKRKKSAGIGLLFLAIGVIGI
                     ICATLI"
     gene            32559..33953
                     /locus_tag="KPH93_000156"
     CDS             32559..33953
                     /locus_tag="KPH93_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687004.1"
                     /translation="MRKIWNRGHRIRASDKHLVYHFSIGTLLFLFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLKLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMENVY
                     HTKEKMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            34137..35333
                     /locus_tag="KPH93_000157"
     CDS             34137..35333
                     /locus_tag="KPH93_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1687005.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            35346..35480
                     /locus_tag="KPH93_000158"
     CDS             35346..35480
                     /locus_tag="KPH93_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG1687006.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            35481..35702
                     /locus_tag="KPH93_000159"
     CDS             35481..35702
                     /locus_tag="KPH93_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687007.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            35906..36682
                     /locus_tag="KPH93_000160"
     CDS             35906..36682
                     /locus_tag="KPH93_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687008.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            36767..37063
                     /locus_tag="KPH93_000161"
     CDS             36767..37063
                     /locus_tag="KPH93_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687009.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            36982..37485
                     /locus_tag="KPH93_000162"
     CDS             36982..37485
                     /locus_tag="KPH93_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1687010.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            37503..38006
                     /locus_tag="KPH93_000163"
     CDS             37503..38006
                     /locus_tag="KPH93_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1687011.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            38124..38522
                     /locus_tag="KPH93_000164"
     CDS             38124..38522
                     /locus_tag="KPH93_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687012.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            38500..40950
                     /locus_tag="KPH93_000165"
     CDS             38500..40950
                     /locus_tag="KPH93_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687013.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            40950..43145
                     /locus_tag="KPH93_000166"
     CDS             40950..43145
                     /locus_tag="KPH93_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1687014.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            43142..44149
                     /locus_tag="KPH93_000167"
     CDS             43142..44149
                     /locus_tag="KPH93_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG1687015.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            44165..45076
                     /locus_tag="KPH93_000168"
     CDS             44165..45076
                     /locus_tag="KPH93_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687016.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            45197..45574
                     /locus_tag="KPH93_000169"
     CDS             45197..45574
                     /locus_tag="KPH93_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1687017.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            45575..46438
                     /locus_tag="KPH93_000170"
     CDS             45575..46438
                     /locus_tag="KPH93_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687018.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            46435..47079
                     /locus_tag="KPH93_000171"
     CDS             46435..47079
                     /locus_tag="KPH93_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1687019.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            47092..47283
                     /locus_tag="KPH93_000172"
     CDS             47092..47283
                     /locus_tag="KPH93_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HBG1687020.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            47488..47676
                     /locus_tag="KPH93_000173"
     CDS             47488..47676
                     /locus_tag="KPH93_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG1687021.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(47784..48143)
                     /locus_tag="KPH93_000174"
     CDS             complement(47784..48143)
                     /locus_tag="KPH93_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687022.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            48309..48524
                     /locus_tag="KPH93_000175"
     CDS             48309..48524
                     /locus_tag="KPH93_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBG1687023.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            48541..48813
                     /locus_tag="KPH93_000176"
     CDS             48541..48813
                     /locus_tag="KPH93_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBG1687024.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            48815..48964
                     /locus_tag="KPH93_000177"
                     /pseudo
     CDS             48815..48964
                     /locus_tag="KPH93_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            49025..49366
                     /locus_tag="KPH93_000178"
                     /pseudo
     CDS             49025..49366
                     /locus_tag="KPH93_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            49491..50228
                     /gene="erm(B)"
                     /locus_tag="KPH93_000179"
     CDS             49491..50228
                     /gene="erm(B)"
                     /locus_tag="KPH93_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG1687025.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            50374..50658
                     /locus_tag="KPH93_000180"
     CDS             50374..50658
                     /locus_tag="KPH93_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBG1687026.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            50761..51639
                     /locus_tag="KPH93_000181"
     CDS             50761..51639
                     /locus_tag="KPH93_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG1687027.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSTTIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLAYSMQ"
     gene            52087..52503
                     /locus_tag="KPH93_000182"
     CDS             52087..52503
                     /locus_tag="KPH93_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1687028.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            52510..52758
                     /locus_tag="KPH93_000183"
     CDS             52510..52758
                     /locus_tag="KPH93_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687029.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            53009..53410
                     /locus_tag="KPH93_000184"
     CDS             53009..53410
                     /locus_tag="KPH93_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687030.1"
                     /translation="MIRPHGAVLPTRMGIVEILTELSKAVCLLIIDSFSNKIYLLFLF
                     RCVIMTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAEQLDISKKVAY
                     SLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            53429..54874
                     /locus_tag="KPH93_000185"
     CDS             53429..54874
                     /locus_tag="KPH93_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HBG1687031.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
     gene            complement(55083..55159)
                     /locus_tag="KPH93_000186"
     tRNA            complement(55083..55159)
                     /locus_tag="KPH93_000186"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55123..55125),aa:Arg,seq:cct)
     gene            complement(55227..56921)
                     /gene="fdhF"
                     /locus_tag="KPH93_000187"
                     /pseudo
     CDS             complement(55227..56921)
                     /gene="fdhF"
                     /locus_tag="KPH93_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(57172..58230)
                     /locus_tag="KPH93_000188"
     CDS             complement(57172..58230)
                     /locus_tag="KPH93_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1687032.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(58441..59100)
                     /gene="cprR"
                     /locus_tag="KPH93_000189"
     CDS             complement(58441..59100)
                     /gene="cprR"
                     /locus_tag="KPH93_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG1687033.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGFGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVLIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT        18830 a        10444 c         9463 g        20542 t
ORIGIN      
        1 gtcttctaaa tctgtatcaa aattattttc tacttttact ttatctttta atcttaatgg
       61 atttacttca atagatttat ctgtatattt aaagtattct tcaataaaac aattttctat
      121 accatagtaa taaacatact taaagtccct taataatctt tttatgaact catatgattt
      181 tccctctttt gatagcaagt atgtttctct tattttttta tcttttgtta tatttataat
      241 taactgactt tttttacatt ctataaatac tccttcttct gaaaaattct ctattaaatt
      301 taagtttata agcttttgta atatatcaaa atttatattt atagtccttg gattcatatt
      361 tagattttct gaaatgttct taattaattg aataatactc tttgggaaaa gaattacttg
      421 tatattcaat ttatcatttt tagaatttat aactttatat tttattgaat agttatttat
      481 gtttattgga atgtattgtg ttatttcaaa ttcaatcatg cttaatatat ctcttttttt
      541 cttagtattt agaacttcaa catttctaat gactatatct tgaatttgaa tgttaaagta
      601 taaattttta tatagtattt ttgatttacc aatctcctct tttacaaatc taactacatc
      661 ttctatatat ttaatcttct caatcgagtt actataattt tttatatcaa agttaatctc
      721 ttttataatt gatttatcta taaaaacttt tttatgactt tttcttaaaa atacaagcct
      781 tatataatca tcatttattt ctaaatttag aattttttta attatagcca tcaccttctt
      841 aaataaaaat aaattttaaa acttacaaca atcaacataa aatattattt tggataaaat
      901 tgcttagaat ttatatttac gcttatatct gttccgttat catttatgac aagttcaaat
      961 ttacctgatg tactctgagg aactggaaca gtgtttagat atcccttttc cttcaatgtt
     1021 tctacactta gagagtctat tatcttttca tcatcattaa ttgccatgct agctgctgtt
     1081 actatattag cggcattcgt atagtctgca tttaatttag cactttcttg aaccttacta
     1141 tacttaacta atgctatact agatataact cctaaaatag ttactactac aatcatttca
     1201 acaagtgtaa atccttttcg ttttttattt atcaattaaa tattctctcc ttttcaaaag
     1261 tttcatctaa taaaatttat ttttctgtct aaaaactagt aattgcatca aacataggaa
     1321 caaccattgc taaaataaaa gttccaataa ttaatcccat aaatacaatt agcataggtt
     1381 ctataaattt cattatttgc tctattttgt tatttaattc attttcataa aatttattta
     1441 ttacatttag acttttatct agcttcccac tttcttctcc tgtatttgcc attgaaataa
     1501 ataattttgg aaaaatattt gctaaattca aagatttacc tatgctattt ccttttttta
     1561 tataattatt agaaatcttt aacctttcat aaataaattc gttgtctata acactagctg
     1621 acatatctat agcttctaca atttgtattc cactgctaat taaaacagac aatgcccttg
     1681 aaaatcttgt tgtagctact aactgattaa ttctttttat aaaaggtatt ttgaatttta
     1741 acgaattaat caattttttt atattatcat ttgttataaa gaaataaatg cttcctattg
     1801 aaaacaatat aaagctaaat attagataag ctaagttatt tgtaactatt gtagatattc
     1861 ctattaaagc tctagttatt aaaggtggtt caattccatt gctggtataa accacctgaa
     1921 aattaggtat tacaaatacg aatataaaca acattgataa gatagataat attataagta
     1981 ttattggata gattgaaata gttattattt tcatttttaa tttgtattct ttatcataat
     2041 aattagataa atcgctcatt attatatcta agtttccact agtttctcct gcttttatca
     2101 tacttataaa gaatttagaa aatgtacctg ttatttgaaa tgattctgct attgagttac
     2161 ctttttgaat atgatttgat actatactaa gcaaattctt tactttttta cttgactgac
     2221 tctcaatgat ttcaaatatt tttgtaattt cacatccaga ttctagcagt atacctatct
     2281 ctttgcaaat tactctaatt tctttatcac tgattctatt taatactaaa taaaactttt
     2341 ctaaattata aaatatattt ttagataatc tcatgtacta taataccttc caaattatat
     2401 taattacaat taaataaact taatattttc taatttaatg cacttaattt tatttcactt
     2461 aaatataatt tacactcaaa catcttaatt aaaatagatt aaataaagac aactgaccaa
     2521 acgaagttta attatgcaat tcagttaaat catttcattt actactaaac actcttctaa
     2581 tgtcgttatt ttttcattta ttagtctttt acaactatct tcaaatgtta tcattccatt
     2641 attttgtgcc acgtctttaa tagtactaga atcctccata tttcttatac atgtctttat
     2701 atcatcatta atttctaaga tttcataaat tgctgttctt ccaaagtatc ctttatcatt
     2761 acatttttca caacctacag ctaccttagt atttacatct ccactaaaat tatcttttat
     2821 cataatctca tggctacagt gattacagac ttttctaacc aacttttgag atataacacc
     2881 tataagagag gcatttaaaa gatatggtgg tatttgcatt tctagtaatc tagctatgga
     2941 agatactgca tcattagtat gtaaagtact tataactaaa tgacctgtta tagctgctct
     3001 tactgctatc ttagctgttt ctgcatctct tatttctccg accattatta tatctgggtc
     3061 ttgtcttaat atagctctaa gacctacatc aaagctcaac cctacttttg aatttacttg
     3121 aatctgactt ataccatcca tcttatactc tacaggattt tctatagtca ttatattttt
     3181 atttattcct cttaaatcat ttaataaaga atatactgtt gtagtctttc cactacctgt
     3241 aggacctgtc actaataata tccctgctct tttatttatt atacgattta tagattttat
     3301 agcttcatct gaaaaaccaa gttctaattt gtcttttaaa aaagtatctc tattgagtat
     3361 cctcaaaact attttttcac catagacagt tggtattgat gaaactctaa tgtcaattaa
     3421 atttccacct aactttatat ctaccctacc atcctgagga agcctttttt ctgttatatc
     3481 cattgatgcc tttaatttta taaccgtaga caatgatgga tagacatcaa ctaaaaactt
     3541 tgatacttcc tttagttctc catctaccct cattctcact attaaatgtt ctttaaatgg
     3601 ttctatatgt atatcacttg cattttcttt tatagctttt tctaaaagat tttcaaataa
     3661 tttttttgca taatcctcat caagttcaaa gctattatag tatttttcaa tcttttcact
     3721 tataagttct tttttctcct tcttcaatat aatttcttta cctgtagcta atctcaagtc
     3781 ctgtaaagca taaatatcct catcttctat ttctacaact aaattatcat cttgaaactc
     3841 aactggaaat acattatatt tttttgcaag tttttctggt attaacattg gtaaactgct
     3901 ttcctctaat ataatcttaa tcacccttta tatactttaa tttattaggt atattcattt
     3961 ttttcataga gatattttct tcagttttta tgcttatttt cattggataa ttggtttgta
     4021 tgctatctaa atatttactc cctttatcta cataattaga tagtttctcc ttatctccaa
     4081 tgcactttat ttcatatggt tgtgctacag gaactccatt tatatttata tggcttcctg
     4141 caagtacaac ttctgaatat tgattaattc tttgaccatt tatttctatg tattttcctc
     4201 cgtttacttt taattcattt ataactttta caagaatctt attatagtct atactattag
     4261 ctatattccc tgttttttca tctatagcat ctatacttaa tattatccca ctacccttta
     4321 catccgaata tgatagacta tctctaagtg tttctatttc tgatatttct tcagaatttt
     4381 tttgattttg tttcattgaa ttaatttctt tatctaactt atctttctct ttggttaatt
     4441 cttttataga ttttcttgtc atttttattt cttttcttat tgacttgtct ttgcttgtat
     4501 aaactgtttc ttcattgcta taatctctaa atatggttcc aacaagtata cctaaaatag
     4561 tacttgctag tattataaac acatattgtt ttttcatact gtattgctcc ttacaaattt
     4621 atttttatat gataattttc tttaattaaa tattaaacat ttacatatgc ttattatatc
     4681 aaagttatat attaaaattc atctttagta tataatatca tacattgaac ttctaccact
     4741 aatttaccaa tatcaagtat gtatttttag atgttcttaa aattatataa actttcaata
     4801 aacattgttg tgatattata aaaaaacata aattagaaaa ggagataata attatgaaaa
     4861 aacaaataat gactttggag attattaata attatttttt atcaaaaaag taaaatggca
     4921 tagcctcaaa gctacaccaa tttactaaaa attatattat aattaacatt aattttaaac
     4981 atatgcgttt ataagttttc ctcatacttt aaaattcttt taattaattt atctcttaat
     5041 ttccctactt catcaaatct catattagga ctatagtaac tttctataat atcatgttta
     5101 gacttggctc tcttaagatt tagcactgcc caacaaatta aatcatctaa aactttttca
     5161 tcaaatttaa tatcttcttc atatttaagt agtttttctc tatttaaaaa ctgatttaaa
     5221 tcaattgtat cttcttcttt aaaagtcgta cttatagtta tacctatgtc taaatcagtt
     5281 aatagtacag tttcaatttt ctctggaata agtggataat gataaacttc tacatttaaa
     5341 cctttttcaa cagctttctt atatactttg tttaataatg tagttttccc agttcctatt
     5401 tctccagcta aataatagac cttatttaca tcttttaaaa tgttttctgt ataatctaaa
     5461 caccctacag gagttatagc tgaaccaaat aaatgctttt ctctttttag atttcctgtg
     5521 cttttaactc catcaaataa cctttttata aattcttctg tgaccaaatt cactttgcca
     5581 taatccatac aatcagaata tttttcttct atgtctctaa agattggttc tgctgatttt
     5641 aaaaacttaa atgccctttt aaatgaatta ctaatatctt tatcgtaaac tgatatttct
     5701 tgtttattct tttctaattc ttctgtattc cagtaatcac ctaaattaat tatctcatcc
     5761 aaagctccag gtagctttgg gtccatagta tgtggagcag ttgcatccat aagtaccact
     5821 cctaatttct ttataactaa accatctaac gaacttgggt cagaaggaca atgatataaa
     5881 tcaatatcat atcctctatg caaaaattct ttataaattt ttttcataag tgaagatttt
     5941 cctactccag gtccaccctt taaataaaaa actcttttta cattttcagg aattatatag
     6001 tcaaaaaaag aatatgagcc ttgtgatgta tttccacccg ggaatgcctt ctttaccttt
     6061 ccttccatat tagcgcctcc tcataattta caatatctct taattaagat attcatatta
     6121 taagaatttt attacagctt tctgattttg acaaatttaa aacttgccat ctaatactaa
     6181 taacaggtta attttatcta attattatag attctcatat tctcatactg taccttattc
     6241 tatctataaa tacaatttaa gtgtccatat tgaaatattt gtattgtaac atagctggat
     6301 attacttaaa tccaattgtt tccattataa ttttatgtta aaataatatt acaaaataca
     6361 tctgtttttc ttcataaacg ggtgaaattc cctatcggcg gtaaaagccc gcgagcctta
     6421 tggcataatt tggtcatatt ccaaagccga cagtaaaatc tggatggtag aagaaaatag
     6481 tatatgagta cctttatgta attttacatg agtaatctat acaaatcctt caactaccgt
     6541 atttattcat gaaattagac acattcaagg tacctaatat acaggtgctt tttttgttgt
     6601 ttattttata attatatcgt acttataaaa tctattaaga ttggagtgtt atcatgaaac
     6661 aaaaatggat agtattgatt atcatctgta ttggtgtatt tatgtctact cttgatggaa
     6721 gtatactaaa tatcgcaaat cctacaattg ctgctgactt taaaattaac atgtcacaaa
     6781 ttcagtgggt tgtaacagct tatatgcttg ttgtaacagc tactatgtta tttttcggaa
     6841 aacttggtga taaagtaggt tcaaatcgtc tatacacatt aggatttttt atatttacaa
     6901 taggttcttt tctgtgtagt atgtcaaaca atttatcaac attaatttct tcaagaatct
     6961 ttcaggcagt tggagcaagt attttaatgg ctactggact tggtatagtt tccaatgcat
     7021 ttccagcaaa tgaaaaagga aaagctatag gaatcactgg agctgtagtt ggtataggaa
     7081 atatgagtgg gcctgtaatt ggaggtataa ttttagaaca ttttggatgg ccttctattt
     7141 ttattatcaa tatacctatt ggtattatag cagtctttct tggcattaaa tttttaccta
     7201 aacctgtttt ggatgagcaa aataagagct ttgatattcc aggtcttcta cttttcgcat
     7261 cttgcactac gcttatatta cttgcaatga atgagaatgg taatacaaga ttgtatttag
     7321 gtataacagc acttataatc ttcttactgt tagcattaag agaagtaaaa tttgaacaaa
     7381 gctttattga cctacctcta tttaaaaata gaaactttac tgtaggcaat gtcattggag
     7441 tagcttgtta ctttcctcaa atggcagtat cttttctact tccattttat ttagaacaat
     7501 taaaaaattt atctcctatg atggctggtt atgtaatgac tgtacatcca cttattatgg
     7561 tacttatagc acctattgca ggttcacttt ctgataaaca cggtgcaaaa aacattctga
     7621 cagcatcttt ttcatttatg accatctcat tagttggaat ggcactttta aaagcagact
     7681 ctccattgta tcttttaatt gtatgtttag ttatttttgg acttgggcta ggagcattta
     7741 gttcaccaaa caatagtagt attttagcag atgttcctcc tcaaaaacag gggtatggag
     7801 gcagttttct tgcaactatt cgtaatttat catttgctct tggaacagca ttttttagca
     7861 gtttttttgc acaaagtctt gcatacaatc aaaagttcaa aagtcacaca tcagcctatg
     7921 ttatagctag caatcagtct tattggattg cagcatcagt gtgttttata gggcttatac
     7981 taactgtttt ctttatgaga aaaacagata aatctatttc ttaaatgcta tttattgctg
     8041 actacataag tcaataattt tttagttgaa ctaatttata ctaattttcc cataccttac
     8101 taaaatttat tagtatttag caaaattact gatatttatt gtttataaac aataaggatg
     8161 cccaaaaaag ttcattaaaa ctattttaag gcatcctata ttttgcttta atattaatat
     8221 aattaaaacc aactattatt acatttatta ttcaaataaa tcttatatca acatttaaca
     8281 ttctaaattt aatctactgt tatttcatat ccatgttgat tgtattgatt tacaatttct
     8341 tcccataatt cttcaacacc agtctttttt agtgaagaaa caggaattac tttttcgcta
     8401 ctttccattt gtaatttttt tcttattata cttatgtgtt tttgatattg ccctctagat
     8461 attttatctg ccttagttgc aataactaca cagttatatc caaagtgctt tatccattca
     8521 tacataagct tatcatctgc tgtaggctca tgtctaatat ctacaagtag gaatatagaa
     8581 cataactctt gtctactttc tagatatctt tccattatac ctccccactt ttctttttca
     8641 gacttagcta tctttgcata tccatagcct ggaaggtcta caaaataaaa ttcattattt
     8701 attaaataaa aatttatagt tctagttttt cctggtgttt gactagttct agcaaagttt
     8761 cttctgttaa gcaatgtatt tattattgat gattttccaa catttgacct accagccaat
     8821 gctatctctg ggattccttc tgctggatac tgtgatttat tcacagcact catagttatc
     8881 tctgaacttc taattttcat tattttcacc actctttaat aaagcttgtt ctaatacttc
     8941 atccatatgt tctacaagca caaactccat tttttctttt acattttctg gtatttcatc
     9001 taagtcagct tcacattctt taggaattaa tacttttgtt ataccagctc tatgagctgc
     9061 taataacttt tctttaactc ctcctactgc taagactcta cctcttaaag ttatctctcc
     9121 tgtcattgct atatttccag gcacaggtct tttagtaagt gctgagatta ctgcaagagc
     9181 catagttata ccagctgatg gtccatcttt aggaacagca ccttctggaa tgtgtatatg
     9241 aatatcattc gtcttataaa aatcagggtc tatgtcaaac ttgtccacta ttgaccttat
     9301 ataagagata cctgtcttcg cagattcctt cataacatct cctagttttc cagttaatac
     9361 aatttctcct ttacctttaa gtacgtttac ttctacttca agtgtaactc caccaacttc
     9421 tgtccacgca agaccattta ctaatcctat ttgagggtta acttctgcaa gttgatactt
     9481 aaacatatct tttccaagat atgcctctag gtcagactta tttataacaa cctcttcaag
     9541 agttgggtct tcaacgtatt ttttggcaac ttttctacaa atcttaccaa tagttctttc
     9601 tagtgttctt actccagctt ctcttgtata atggttgatt atacttctta aagtttcatc
     9661 atctattttt ataaagtttt cttttaaagc atgttcttta atctgttttg gaagtaggta
     9721 tttttttgct atatttagtt tttcttcttc tatatatcca gatacttcta ttacttccat
     9781 tctatctaat aatggtcttg gtatattacc tagactattt gctgttgtaa cgaatagtat
     9841 ttttgataaa tcaaaaggta tttctaagta atggtccaca aaatccttat tttgctctgg
     9901 gtctaatact tctagcattg ctgaagctgg gtcaccttta taatctgctg ccatcttatc
     9961 tatctcatca aataagaata caggattttt tgtttgggct tcttttactc cattaattat
    10021 tcttcctgga attgaaccta cataagttct tctatgacct cttatttctg cttcatctct
    10081 aactccaccc aaagatattc ttacaaattt tctatttaat gctctggcga ttgattttac
    10141 tatagatgtc tttcctgtcc ctggaggacc aactagacat attataggac ctttaagtga
    10201 cttagcaaga gttcttatag ctaggtactc tagtattctc tccttaactt tttctaatcc
    10261 ataatggtct tcatctaata tatcttttgc cttagttata tctaatttat cttttgtctc
    10321 tttattccat ggcaatgaga atatagtatc taaatagttt ctacttacag atacatctgg
    10381 agacatagaa gatatttttg aaaatttatc tatctctttt tctattttct ctttagttgt
    10441 cttaggtgct tttatttttt taagcttttc tctatactca tctgcttcag agtttatatc
    10501 ttcttcttca cctaattctt tctgtatagc ttttaattgt tctctaagat aatattcttt
    10561 ttgaacctta ttcatctgtt tttttactct taaagttatt ttcttttcta tttttaatat
    10621 atctatttcc tctaataata tagaataaat aagttcaagt ctttttctaa tatcaaattc
    10681 ttctaatatt tcctgttttt ggcttgattt tagatatatg tttgcagcta tcgtatctat
    10741 aaatctatct acatcttcta tatcagctaa agatattaat atttctggtg ataccctatt
    10801 tccaatattt atatactctt caaaggcatc aaatacattt cttacaaatg cttcaatttc
    10861 aacttctgtt tcactatcca aattatcact atcaaatact atttcttcta ttactgctct
    10921 gaaatatcca tcttcttgtt ctattttctt tactctaccc cttgatacac cttcaaccaa
    10981 aacacgaact gtatcccctg gtaattttat catttgctta actttacaga ttgttcccac
    11041 atgataaaaa tcctcttctc ctggctcatc tacctctgct tctttctgag aagttaaaaa
    11101 tataagttct tcatccatca tagcttggtc taaagcttta agagatatct ctctacctat
    11161 atcaaaattt aatatcatat atggaaatat agctaatcct cttagaggaa ttagtggtaa
    11221 ttcatgatct attttagtat aattttgttc catcaacatt actccttttc tagtctaatt
    11281 gctttgcttt taatattata tatttgaaca agttaatttt caacattaat gataaattct
    11341 tagaatacta tcatactata ttatcttttt attatgatat aatattaata ttagtatact
    11401 tagatgttat ttatcttttg gaggtttcta ttatgttgaa attgttttta acctttttaa
    11461 aaattggtgc gtttacattt ggtggtggat acgctatggt tcctcttata caagatgaac
    11521 ttgtaaacaa acaaaaactt ttagagcagg aagaatttat tgattattta tccatagctc
    11581 aaagttatcc tggagtactg gcagttaata tatctgtatt attaggatat aagctttatg
    11641 gtataccagg agttcttata tgtaccttag gctctgcttt accatcattt tgtatagtct
    11701 tagtcttatc gtatctatac tttaaaaatt catcatctaa aattttagat ggatttttta
    11761 aaggagttgc gcctgttgta attgctctta tattgtattc ttttacgagc atgttcaaga
    11821 aactaccaaa gtctaaaact aacatactac tattaattat agcagtttta tctgttggtg
    11881 tattcaatat aagccctatt tatttaataa ttggaggagg tgtatatagt ctatgtcaga
    11941 aataatacta ctcttcttaa tattccttaa aataggggca tttagttttg gtggcggtta
    12001 tgccatgcta ccatttatcc agcaagaatt tatatctaaa tatcacttta taactaatca
    12061 agagtttttg gatttacttg ctatatcaca atctacacca ggtcctgtag caataaatag
    12121 tgctacattt gtaggattta agactgctgg cttctttggc tctctagctg caactattgg
    12181 tgttacaatt tttgcattaa taggtctaac tattatatca aagttatttt ctaaatttaa
    12241 aaataataaa aaggtagaaa acttattgac agttcttaga ccgattactc taggattcat
    12301 attatcagca gctttttcat ctgctaaaga agttaattgg gatttatatg gaataattgc
    12361 atttatagca tcattttatc ttttatataa aggaaaaatt ggttccattg caattgtatt
    12421 cgtatttggt attctaggta tattactcac atttttataa taatgatacc tattttatat
    12481 ttagcatatt agcataaaaa aaggagcttt taaagctcct tttcatcatc ttatgcactt
    12541 tcctcttgat ccttaagtac tatgatagga tttactgagt cttcgttcac agatttctct
    12601 gttactataa ccttctttat attatctcta gatggtactt caaacatagt ttccatcatt
    12661 acgctttcaa ctatacttct aagtcctcta gctcctgtat ttctttctat ggctttttta
    12721 gcgattgcac gaagagcacc ttcttcaaat tctagctcaa catcatctaa ctccaataac
    12781 tttttatact gttttactag agcattttta ggttcctgta atatctgcat taatgcatct
    12841 tcatccaaca actctaaagt agctaaaact ggtattcttc ctataaactc tggtattata
    12901 ccatatttta ataaatcctc tggaagtact ttttcataca acttccctaa atctaattct
    12961 tttttacttt ctattttagc tccaaaacct agtgttttat ctccaccacg tttttgaatt
    13021 atcttctcta atccatcaaa agctccacct aatataaata atacattagt agtatctatc
    13081 tttaaaaatt cttgatgagg atgttttctt cctccttgtg gtggaacatt tgcaacagta
    13141 ccttctaata tttttagaag tgcctgttga acaccttcac cactaacatc cctagttata
    13201 gatgggtttt cagattttct tgcaatctta tctatctcat ctatatatat tatacctctt
    13261 tcagcttttt ctatatcaaa atcagcagct tgaataagtt ttaaaagtat attttcaacg
    13321 tcttctccaa catatccagc ttcagtcagt gatgtagcat ctgccattgc aaaaggtaca
    13381 tttagagttc ttgctaaagt ttgagcaagc aatgtcttcc cagaacctgt aggtcctaat
    13441 aaaagtatat tactcttttg aatttctata tccttgctac tagatttttt actatatatt
    13501 ctcttgtaat gattataaac tgcaactgat aaagcttttt tagctttttc ttgacctatt
    13561 acataatcat ttagaatctc catcatttcc tttggttttg gtaaagatgt agtatcctca
    13621 tctatagtat cttctatctc ttcttgaata atttcgtcac aaagttcaac acactcgtca
    13681 caaatatata catttggacc tgcaattaat cttcttactt gatcttgatt tttaccgcag
    13741 aatgaacact ttaattgtcg tttttcttca tatttagaca tattaacacc tctctctatc
    13801 tacggtctct ttgtaaatac ttcatctatt aaaccatatt ctttggcttc tagtgcactc
    13861 atgaagttat cacgttcagt atccatttta attttttcta atggttgacc agttctttca
    13921 gataatatct catttaaagt ttcttttatt tttaaaattc tctttgcatg aatttctata
    13981 tctgttgctt gaccttgagc tccacctaat ggttgatgta tcattatttc actatttggt
    14041 agtgctagtc tttttccttt tgctcctgct gccaacaaga atgctcccat agaagcagcc
    14101 atacctatac atatagtact tacatctggt ttgatgtatt gcatagtatc atatatagcc
    14161 attccagaag ttatacttcc tcctggagaa tttatatata aatgtatatc tttgtctggg
    14221 tcttcagctt ctaaaaataa tagctgtgct actataagcc ctgctgtggc atcgttaact
    14281 tgatctccta agaagattat tctgtctttt aatagtctag aaaaaatatc ataagatctt
    14341 tctcctcttc ctgtttgttc aacaactaca ggtactaatg ccatggtttc ataccccctt
    14401 tattctatat aatttacagt agcttttttt aagctactgt aaattattat acgcatttaa
    14461 ctaaattata tttgcacatc attatatact aaacatttca taacgtcaac tatattagtt
    14521 agcgtttatt ttattaagct attgttgcat tatcaactag taaatctatt gtttttctta
    14581 tttttatttg accttttata tcttctttgt cagcatctct taaagaagct tctatttttt
    14641 ctacttccat attgtaagca gaagccattt tttctaactc agccttaaat tcttcttctg
    14701 ttgcttcaat tccttcagct ttagttattg cttctaatac taaagaagac ttcactaatt
    14761 ttttagcatc ttcttttctc tcttctctta attcttccat agttctacca gtcatttcta
    14821 ataattgttg taatccaaat ccttgatatt gtaattgata gttaagttca tttaacatat
    14881 tatctatttg atgttgtatc ataacttctg gtatttctat ttctgtattt tcagcaacct
    14941 tttctactac tgaatttcta gtttctgcat ctgctctatt tttagcttct tcttctaact
    15001 tagctcttac atctgctttt aattcatcta aagaatcaaa ttcactagta tctttagcaa
    15061 attcatcatc aagtgcagat aattctttta cttttacatc atttattttt acattaaata
    15121 caactggttt tccagctaaa tcttgtgagt gatattcttc tgggaaagta acattaactt
    15181 ctacttcttc accagctttt ttacctacta attgctcttc aaatcctggt ataaatgtgt
    15241 ttgaacctat aactaagtta taattttcac ctttaccacc atcaaaagct acaccatttt
    15301 caaaaccttc gaagtctatt atagctgtat ctccatcttc taaagctttg tcttctacac
    15361 ttactaatct ggcatttttt tcaaccattt cttgtagttt agcatcaaca ttttcgtcac
    15421 ttacagtatt ctcaacctta gctatttcta tacccttgta gtttcctaat tcaaactctg
    15481 gtttaacttc tacattaaga accataacca acccattgtc tttacttatt tcttctatat
    15541 ctaaatctgg attgtctatt gggtctatgt ctaattcctt gatagcttct ggataaactt
    15601 ctgggaataa tatttctata gcatcattat agaatatacc ttttccatat tgagtctcta
    15661 taacaattct tggagcttta ccttttctaa atccaggtat attgtattta tttctgcttt
    15721 tattataagc cttatttact gctgcttcaa atttattgtt atctactgtt atttttaaag
    15781 taactttatt accctctttt ttaattagtt ctgctttcat ccttctaatc ctcctagtga
    15841 tatttatgta atacccgata acggtattat aattcattct atttataatt caaccactta
    15901 attatatcac ataagttttt ttatttacaa gtgttccatt aatatcgttg tacttatttg
    15961 ataaaattta attttttgag cgttttttgc cattttttta gataaataca cctttatatg
    16021 cgtatttctt ctatatttat gttatcttct tctatatcca taatgacaaa acttttatac
    16081 gatactcctc ttggaagtga agtacttcca ggatttatgt aaagtacacc atctttaact
    16141 tctctaaacg gagtatgggt atgaccaaat ataactatat cagcatcaat ttcagttgcc
    16201 tttttttcta acatatgcgt tccatatttt acaccatact tatctccatg acataagaat
    16261 atggttttat ttgccacttc aaagacaact tcttcctcaa cagcatcaaa gtcacaatta
    16321 cctttaactg caataatacc tacattagtc atgctatgaa tatatcttga atctgtaaag
    16381 ttatctcctg catgaattat taggtcacat tcttttaaat atggtattgt tttttccata
    16441 tttttaatca tcatatgtgt atcgcttact atgcctattt tcattacttc actacccttt
    16501 ttataaactc ttgtttcata agttttagtg cgtttgctct atgactgatt gaatttttta
    16561 taacactagg tatttcacca aaagtcttat catatccttt tactataaat aagctatcat
    16621 atccaaaacc atttttacct ttttcttcaa atcctatcat tccttcacaa attcctctta
    16681 ctacaaattc ttttccatca ggaaaaacta ctgctattgc agagacgaat cttgcatttc
    16741 ttttgctcat tggtacattt tgcatagctt ttaacagttt ttttctattt tcttcatccg
    16801 ttgcatgttc tcctgcatat ctagctgaat aaacaccagg ttttttgcct aaagcatcta
    16861 cttctaagcc agaatcatct gatattgcta taagtttagt cttttttgct attgttctag
    16921 cttttatcaa tgcattatgc tcaaatgttt ttccatcttc tactatctct atacctgcta
    16981 aatcaacatc ttttaaagag tacactttat attcaaaatc ttttaagatt tcacctattt
    17041 cttctagctt atgagcatta ttagtagcaa tcactacttc attaccataa tccataccca
    17101 atatctcatc tgctatttca cctaaagctt ctctttgaag ccttattaat tctttattcc
    17161 ctttttcacc taactctaac aataaattta aatctgctct cgaaaaaggt ttctcttctc
    17221 ctgtcccctg tacttctaca aactcacact tatcagtcat tattatattc atatctacct
    17281 gtgctttaga atcttcttca taacataagt ctagcatgtg tattccattt acaataccaa
    17341 cactaattgc agatacaaaa tttgttattg gcatctcctt tattgtcttt gctttatata
    17401 atttatatat tgcttctgca actgctataa atgcccctgt tatagatgca gttctagttc
    17461 caccatctgc ttggatcaca tcacaatcta cccaaatagt tctttctcct aatttattca
    17521 agtctacaac agaccttatt gctcttccta ttaatctttg tatttcttgt gttcttccat
    17581 caatttttcc cctagatgca tctcttatct ttctttgttg tgttgctctt ggtagcatag
    17641 aatattcagc atttatccat ccagtaccac tatttcttaa aaaaggagga actttttctt
    17701 ccactgatgc agtgcatata acctttgtct ctcccatttc tataagaaca gaaccttctg
    17761 catactttgt ataatctcta gttatcttta caggtcttat ctgttcgtta cttctatcat
    17821 aacttctggt cattttttat atcctttctt atattttttg ttttttataa atttattttt
    17881 ataattttta attttgtcaa atattaatta ttgtcatttt tcgtaatctt attagcttac
    17941 tgaactattc tatcattaat tatagtagtt ttctactaaa ttaagcgact ttttaactct
    18001 ctaatacagc ttttacttca tctttcgatg ataactttaa taattctaaa aatctctctt
    18061 catctataac tttaattcct aaatcatttg ctttagtgag tttagaacca gcttcttcac
    18121 ctgccaaaac aaatgttgtc gatttactaa cactagatgt agcttttcca cctctttttt
    18181 ctatcatatc ttttgcatca tttcttttta atgtaggaag tgtaccagtc aatacgattt
    18241 tcatcccttc aaatattttt tctacttttt cacctgtatt atccaatgat tttgtattta
    18301 cgcctacatt ttttaacttg tttataacgt tcatattttt gtcttcctta aaaaacttga
    18361 caacgctgtt tgccattatg cttccaaatt cctctaattc ttctagttgc tcactagttg
    18421 cagaaattac ttcatctaaa tccttaaagt tatatgcaag tatcttagct gctttaactc
    18481 ctataaattt aatacctaat ccatttataa gtctccataa atcattttct tttgattttt
    18541 caattgaatt tattaaattg tcagcagatt tttcgcccat tctctctagg tcaactacat
    18601 cttccttttt tatataatat aaatcagata catcttgaat aattttttca tttaatagta
    18661 gtgtaattat agattctccc aatccatcta tattcattgc atcccttgac acaaagtgta
    18721 taattcccct tcttatttga gctggacaag acatatttat acattttaca gcagcttctc
    18781 cttctaatct tattgttggc tctgaacaga caggacactt ttctggcatt ttaaaatcta
    18841 tttcatctcc agtacgttct tcttttataa cttctaaaac ttgaggaatt atatcccctg
    18901 ctttttgaac taatactgta tcatttattt ttatatcttt ttctcttata taatcttcat
    18961 tatgaagtgt agctctactt acagtagtac ctgcaagtct gactggttca agaattgctg
    19021 aaggtgttat tgtaccagtt ctcccaactt ctactattat atctaatatt ttagttttct
    19081 tcttttctgc tgggaattta tatgcaattg cccatctagg acttttggct gtatatccca
    19141 tttcgtctct ttgttttata ttgtttactt ttattaccat accatctatt tcgtagtcta
    19201 aattacccct gttatctgtc cagtattcaa tatgttcaat aacttcatct atacttttac
    19261 aaactttata actctgactt actgaaaatc ctagtttttc taagtactct agtgattcac
    19321 tgtgactttt taaatcatat tcttctatat tttccatatt aaatacaaat atatctaatg
    19381 gtctctttgc agttagtttt gggtctaatt gtctcaatga accagctgca agattccttg
    19441 gattagcaaa taatggctgt tcttgttctt cttgttgttc atttactttt ctaaaattat
    19501 cttttgatat atatacttct cctctaacta ttagttcttt tttatcattt atcttaagag
    19561 gtatgctttt tactgtcatt aagttttgag aaatatcttc tcctacaatt ccatctcctc
    19621 tagttgctcc tttttcaaac tctccattat tataagttag acctacagaa aggccatcta
    19681 ttttaaattc aacaacatat tcaacatttt ctcccacata ttcttgaacc ctcttatcaa
    19741 aatctctcaa atcttgctct gaaaaagcat ttgataaact caacatagga gttttatgta
    19801 caatttgatt gaatttatct aatggcttac ctcctaccct atttgttgga gaatcaattc
    19861 tctttagttc tggatgttct tcttctaaag aaattaactc cttcattaac ttatcgtatt
    19921 cataatctga tatttctggg ctatcttgat tataatacct ttcattatgg tagtttattg
    19981 tctctataag tttattaatt tcatctttta cgctcacttt ttgcacctct tcatattttt
    20041 taaattaaat tattttgaaa tttccctata attaattatc tcctatttat atatttttta
    20101 gtatatcact tttttgactt taaatttatt acaaaaaaaa ctcttattag tatctctaat
    20161 acgactaata agagttttta tttttatcaa aattttttat ttttaatagc ctcttgatat
    20221 gtctacaaca ttatctacag gtttattctc cattatatag tgtttcaaat tattataaac
    20281 catattaaat gttctctctt tgtttttatc tgacacccaa gaattatgag gagttactat
    20341 gacattttcg cactcccata acttattatc cttgtttaaa ggttcacttt caaatacgtc
    20401 taatgctacc cctctaaact tctctacaca ttcttctaaa tctttctgat taactgtatt
    20461 tcctctacct acatttataa atacagagcc atctttcata agtttaaaca tatctttgtt
    20521 tactattcca tctgtacttt ttgtagaagg cattgctaca actactatat cacattcttc
    20581 aaatacaata ttcatatcct cagtggcaaa acacttatca aagtattttt tactacttcc
    20641 atcagtattg acaccccata tttctagacc aaaagctttt aatctttttg ctgcttctga
    20701 agatatagta cctgttccta aaaatccaac tctttgtcca tacaattctg atacagaaaa
    20761 atccatcttc cattctttat tttgttgttg tttaaaaaat tttgcactat ttttatatat
    20821 ttctaaaatg tacaatacaa tactttcacc aataggtaca ctatatccgc ctctattatt
    20881 agctatctta atatttctct tcaatacctc atcttttgga agttggtcta tacctacact
    20941 agttgtctgt atatattgta aatttttcat cttagaaata tctaatctct caaatggatt
    21001 gtatgtaact agtacatctg ctgtatttac atcctcatta ttttcaatac tatcttcaga
    21061 gtaatgtatg acatcatagc ctaattctct tattttatca aatttttctt ttccataatt
    21121 tattgtaaat aaaactttca tctcaaatct cctccatcta cccacattta aaatgttata
    21181 ttctaaagta taatatgata ctcaatgtac attagttaaa attactagaa gatatcttaa
    21241 atttacttat gttcaattgt ttttcctaaa gacatagcat tacgttcttg aactaaattt
    21301 tctattaaat tattcaatgt ccaatcaata gatgtctccg ttaccatagc tgtaaaaggt
    21361 ctatttattc tcactttttt tagcccttct aaaaatgcat ttacttgtgt acttggagtg
    21421 ttattaaata ttacagactt actcttaatc ccattttcac attcatttga tatatttcca
    21481 tctaaaatgt ctcttattat gttctctcca tttttatcac ttaagatgat atggtctctt
    21541 attccagcaa agttacttac atttttaatt aatgataatt cttttttatt aaagtttaca
    21601 atcataatac acttcctatt gtgtatttta tctaggttat tattatttat tttttcaaat
    21661 gacattttat ccatcctttc atttatttat atactttaat ttaccccata catttaaaat
    21721 aatcaatttt tatagttaat ttttatatta atacccaaat tatatttaaa tagttaggct
    21781 atccaaaact aatatataaa attagaataa ttagattaat atctacaaat cataacctaa
    21841 aggagagata ccatgaatac attactaaac aataaattta gcattaaaaa cttttcaaat
    21901 gcttgggtta tagtacttgg ttgtatgttg atacaagctg ttccatttag tattgtagca
    21961 agtatacagc ctcaatttat aagttatgtt gtagaaggag aaggttttag tttagcttct
    22021 ttttctctaa tattcactat tggtactatt gcttcagcaa ttgcatcacc ttttataggt
    22081 gtattatttt ctaaagtcaa tattaagata ctatatttat taggttgtat aatttctagt
    22141 attgcttttg ctactttttc tatatgtact gaactatggc aattttatac tgtatcatgt
    22201 attgttcaaa ttggcgctac aattatatct gctataggaa tacctgtttt aataagtgct
    22261 tggtttgatg aagaaagcaa aggaaaagct ctgggctttg cttttgctgg tggttctatt
    22321 ggtagtatat ttctacaacc acttgcagtt cgcttattag caactcaagg atacagtcgt
    22381 tcttatctaa tatttggaat tatagcatta atagttggta tacctgtaat attattcata
    22441 ctcaggatgc ctaaagatat taatgaaaag gtaaaatcta aaggtaaaaa agaaaaagca
    22501 tctgatacaa tttctccttc agtatggggt tatacattaa aagaagctaa aaaaattaaa
    22561 tatttttgga ttatgacgat tggatttata tttgttggta tatatgtttc atcattatcc
    22621 gtacaatatc caacttatct tagaaattat ctaaatccta ttctgatagg aacagtaggc
    22681 tccacatttg ctttatttgc tctcattggt aacttatttg gaggattatt gtttgataaa
    22741 ttcggtcaaa caaagccact tattatatct tttgtattgg ttcttatagc aactttatca
    22801 ttactattgg tagataaatc ttctgctttt gcatttatat ttgccatttt gaaaggtctt
    22861 tctgtatttg cttatatgat tggtcctgct tatttaactg gctctttctt tggtcaaaag
    22921 gagtttggta gcatatatgg aatcgtacag ttaatttttg cagtgggatt tgcattaggt
    22981 tcaacattat ttggattgtt agttgataat tttggataca gattttcttg gtctattata
    23041 attttatcag ttataattgc ctatacttta cttattataa cttctatagg aatgaaaaaa
    23101 ttgaataaag aaagattaga taaactaatg caataattga tttttagata tataaatatt
    23161 ataataaaaa agatggagct tttaatcata aataaaactc catctttttt atattacaat
    23221 atagctaatt ttctcttatt tttaacttta ctatctatat cctcatcctt aactatagta
    23281 aatgccttag ttggacaaac atttacacat gcaggtcctt ctggcgattc tatacaaaaa
    23341 tcacacttat ttgctgtcat tctttctcta aaacaaagct ttccttcatc tacatctttt
    23401 aatgtaacta tgtctattgc acctatagga catgctaaca tacaagtctt acatcctata
    23461 caattttctt catttatttt tattgtgttc ccaaccctaa ctataccacc atttggacat
    23521 gcctttgcac aaggagcatc ttcacaatgt ctacactgta ttggagcact tacttctttt
    23581 gtctttatga cagaaagttt tggattaaaa tttatttcat ctggattttg gtaaaaaata
    23641 tcttcatctg aatgtgcaac tacacatcct atcatacaag ttctacatcc tatgcattta
    23701 tcagggtctg ctataacaaa atagttcatc acttagccac ttcctttcct atacaatatg
    23761 tagtgtgtaa tagatgatga gatttttctc ctaatggttc taccaaatat tcatcataca
    23821 gttttttaat atcaggattc tcatgagatt ttctaattgc taaatgttca tcatgcacat
    23881 atgttgcttt tgttctattt tcataggcca attccttata ttcttctagt aaaatttttg
    23941 gttgacctcc accacttaca cacccaacag gacaagtcat aacttcaata aaatctacat
    24001 ctaattttcc tttttccaag tcttcaagca ctggtattac attttttaat ccacttacta
    24061 ctccaactct taagtctaag tctcctattt taatagttga ctttctaaaa ccattttctc
    24121 ctcttacttg ttttacttct acatcaggaa tactttctcc agttataagc tcatatcctg
    24181 ttcttatagc agcttccata actccaccag taacaccaaa gatagttcca gctccactat
    24241 aactacccaa aggactatca aatttttctt ctcttaaatt tttaaaatca attcccatgt
    24301 cttttattaa ataagctagt tctctagtag ttaatactac atctacatct ctatatccac
    24361 tagaatccat ttcttctcta tcacattcat attttttaca tgtacatggc attatagaaa
    24421 cactaaatat ctttgatgca tctacattat ttatctctgc accataagtt ttaaatattg
    24481 caccgcccat ctgctgaggt gatttacaag aagataaatg attagttaat ttaggataat
    24541 ttttttccat aaattttacc caagctggac aacaagatgt aaacattgga agagttccac
    24601 cttctgtcac tcttttaatt aactcactac cttcttccat tatagttaaa tctgctgcaa
    24661 aattagtatc gtagactcta tcaaaattta aagctctaag cgctgctgcc atttttccag
    24721 cacctaagta tcctaaataa tatccaaaat cttctcctaa agcaactcta actgctggtg
    24781 cacattggac catagtaaat acatttgggt cacttaaagc tttcttcacc ttatctatat
    24841 cacatacact gtatgctgca aatataggtt catttataga ctctggtatc tctctttctt
    24901 gttttttctc ttgtaaaaac tcaaatcctt catctataac tgaggcataa gatttacaag
    24961 tttgaacaca ttgaccacac gtaacacatc tatctaaatt tatctcttgt ggctttccct
    25021 cttcaccttc aatagcattt actggacaga cctttgaaca ttctctacat ccagtacata
    25081 aatccttatc tatatttatt atcataaacg tcacctaccc ctgtatcctt tatttatttg
    25141 ttaacaataa actaagtgca gcttgtctat tcttagcttt tttgtcatct attggagtaa
    25201 caagttttaa tgcttcctga ggacatgcac ttatacaagc tattttttca ttgtctttac
    25261 ataagtcaca tttataagct atctttttat tttcatctaa aattacttgt tctacttctt
    25321 taccatcttc atattgtgga agtaagtcta tagctccaaa aggacatgct agcagacaag
    25381 ttttacatcc tatacacttt tcttcattta tagacataat attattttct ttgactatag
    25441 ctttttgtgg acatgagttt aaacaaggag catcttcaca atgtctacac tgtattggca
    25501 tacagaaatt gtcacctttt actaaataaa gtctaggtat aacaggtata tcaactgttc
    25561 caactgtata acccacatta ttatgctgat tatgcattgt aaaacaagct acttcacaag
    25621 ctctacaacc tgtacatttg tttgggtccg ctattacaaa agagcctatt ttatttttca
    25681 tattatccca cctttcaatt tgtatatttt caaccctcta gttactttca tcatatatat
    25741 ttatatattt cactattttc tcagtactta tatttcgttt acattttttg cacaaattgt
    25801 atgtgttatt atacacctta tcagtatcat caattaatac tccacagatt tcacactttg
    25861 aatatttttg ctcttctttt ttagtacaca tatacttatt ctcacttttt gataactctc
    25921 tgtggatttg cataaatatt catccgtctt cctcttacaa atccaataag tgttatattt
    25981 aattttttag aaagctctat tgataagtta gtcggtgctg attttgatac tacaatagga
    26041 atttgagtca tagctgcttt taatatcatt tccaatgata ttcttccact tacagccaca
    26101 attttatctt ttaatgttat gtcatttaaa acacaaaatc caatagcctt atccattgca
    26161 ttatgccttg ctacatcttc acaagtgact atttttttat caaaattaaa tacagatacg
    26221 ctatgtaccc caccagtatt tttaaataat tctgatgaat ttaaatttct atccattata
    26281 ctgtaaatcg tatcataatt tatttttata ttgctctcta cagaagcaca tgttatatag
    26341 tctagtgaat ttaaaaatat aatttgctca tcttgatttt tttgtttctc ttgaatagtt
    26401 acttttgcaa aacgttcact ctcatttatc tcaaatttta ataagctttt tttgttttct
    26461 atgtatcctt ttgttttcaa gaatcctact ataagttcct ctaaattata tggtgtacat
    26521 aaaaatgtat ttacatattt atcatttaaa ataaaactaa gtggatattc tgcaattatt
    26581 tcttcattat ctattgatac ttcatgttca tttattttgc ttacttctac attaaaagaa
    26641 tttaaatatt tgtagtccac tagactataa ctattttcaa aaactttatc cttactattt
    26701 tcttgtaatt tagtttttga gtatatattg ttcataaaat attagccttt ctgcctttta
    26761 taaatcttaa atctaataaa ttattagata tttgtagctg tcattttgtc tttcaaactt
    26821 tgatactctt ctcttacaca tttctctgct aattcttggt cttcaagttt ttcaagttta
    26881 acagcacaat acttatactc tggtgtttta gatatagggt ctaaatttgc tattgtaagt
    26941 tcattacaag ctccaaccca ccactgataa gtcatatatg ttgcaccttc cttaactcta
    27001 tcagtaactg ttgctcttgt tattacactt cctcttcttg aactaattct aactaattca
    27061 tcatcttcaa ttcctaattt ttcagcatca ttagaattta tttgaacacg tcctggttca
    27121 tcttctaatg aactaagtgt tctacaatta ccagtcattg ttcttactga atagtgtcca
    27181 acttctctta cagtgcataa gctaaatggg tattcatcat cttcaacttc cataggagtt
    27241 ctctgcgaac cgtggtagct agaagttcga ttatcctctt gggagcgttt aatgccgtaa
    27301 attaaagcat aagtgcttgt tatgtttaat tgttgatact ctctggtgta ccctcataca
    27361 ccgtagagtg ttagcaggtt gttagcaaca aaggctaatt taaagcttta atttatcaga
    27421 aaacattaca aacaaactat aaatcattta tcactagaaa tcaagatact cgtatgttat
    27481 agacgttcag aaatgagcgt ctatttttat accaaaaatg aaaggagtat taaaatttat
    27541 gaaaaagaca ttgaaacgat tgtgtacggg cttcttagct cttgcaacta tcgttactgc
    27601 tttaccgact acacccgttc atgcagaaag taagcaatac tggacagaaa gtgcagagcg
    27661 tgtcggtatc attgaaaaag taatgaatga cggttctatc ggctcgacat tcaatgaggg
    27721 catgatgaaa gttgagggca aaacagccta ttgtatcgac attaacacag attttaggaa
    27781 tggctacaag accagagctg acgcaagctc acgcatgaat gccaaccaga tttcagatgt
    27841 ggcgttatcc ttagagtatg tcaaacagta tggcgaagct cacaaggaac tgaactacaa
    27901 gcaagtctat ctgttggaac aatgtgtagt ctggcagaga ttgagcgtac atcttggctg
    27961 gcaatgcgat aacgtgcgag cttcttatga tgaaattcca aaggcaacgc aggacgaagt
    28021 tttctctggt gcaagaacct ttgtcaaaga aaataaagga cgctatgaat gtggcggtta
    28081 tatctactct ggcgaggggc aggaattggg gcaattctgg gcgaagctga atgttggaaa
    28141 tgcgaaactt aaaaagactt ccagtaatgc cagcattaca gacggtaacg ggaattactc
    28201 aatcgctggt gcgacctatg gtgtctttac tgataaggac tgcacgaaac agcttgccac
    28261 ccttacgact gatgaaaacg gaaatacaga tgttgcagag gtaaaagcag gcacagtcta
    28321 tatcaaggaa ttatccgcac cagcaggata taaagtagat aaaactgtat attccttaaa
    28381 gattgaagct ggaaagacgg cgactttgaa cgtatctgat acgccaaaag taacggacac
    28441 tttgattgag cttttcaaga tagatatgga aacaaagaaa gacaatccgc aagggaacgc
    28501 ttctttagca ggtgcggaat ttacatggaa gtattatgct ggcttctata acaaagacaa
    28561 tctccctgcc gaagctactc gaacatgggt tacaaagaca atcgctgaaa cagacagcta
    28621 cgggacaact cattacatca caaaattagc ggacgcatac aaggtgtctg gcgacagctt
    28681 ctatatgcaa gacggaaaag ccgttcttcc acttgggaca ctaaccatag aagaaacaaa
    28741 agctccaaac ggctacttgt tagagggtgc atatatgcag gctggcgata agtccgaaca
    28801 gataaagggc ttatatgtaa cacagattac cgaggacgac gaccttgccg tattatctgg
    28861 aagtaaccag ttttccgtat cagacaaggt tatccgtggc ggtgtgaaaa ttcagaagcg
    28921 agatttagaa acgggcgata tgaagccaca aggaagtgca accttgaaag atactgcttt
    28981 tgacatcatt tccttaaatg ataattctgt tttggttgag ggcaagctat acaagaaaaa
    29041 tgaagtggta aagacaattc gtaccgatat tgagggtgtc gcttctactt ctgctgacct
    29101 cttaccttat ggaaaattcc gtattgttga gagtgaagct cccgacggtt acttaactga
    29161 cggtgcaaag ccgattgatt ttacaatcac agaaaatgga aaaatcgtgg acttaaccga
    29221 caaagcccat tctatctata atcagattaa gcgtggagat attgagggtg taaaaatcgg
    29281 tgcaggcaca cacaagcgtc ttgctgatgt tccctttagg atcacaagca agacgacagg
    29341 agaaagtcat atcgtggtaa ctgacaataa cgggcagttc tccacttctg ctgactgggc
    29401 ttctcataag cacaatacca acgcaggcaa gaccagcgag gacggtgtgt ggtttggaac
    29461 ttctgaacca gacgacagca aaggtgcgtt accttacgat acttacgtca ttgaggaatt
    29521 acgctctgaa agcaacaaag gctttgaact tatcccacct tttgaaatcg tggtatcaag
    29581 aaataatctt ttgattgatt taggaacgct gactgatgaa tacgaaaaag aaatctctat
    29641 ccataccaca gcgaccagca aggacggcga aaagactatc cttgcaggaa aagagattac
    29701 gattgttgat actgtcaaat tagacggact tacaaaaggc acaaaatatc agctcaaagg
    29761 ctggcagatg ttaaaagagg aaaacgccga gcttatcatt gacgggaaac gtgcagaaaa
    29821 tgattatacc tttgtcgctg atgaggaaga aatgaaagtg gaaattgcct atacattcaa
    29881 tgcgtctgct ttaggtggca agaaccttgt tacctttgaa gaattgtatg atttgagcaa
    29941 tccagacgaa cccgtgaaag ttgcggaaca caaagacatt gaggacgacg ggcagaccgt
    30001 tttaatcaca gagcgtatca tcaaaattca tacgactgct acgaataagg acggcaacaa
    30061 agagcttgaa gctggaaaaa acgttacaat cattgatacc gtaaccttag agggcttaga
    30121 agttggtaca cagtacaaac ttgtgggctg gcagatgttg aaagaagaaa atgcagaact
    30181 tcttatcaat ggaaaatgtg tggaaaatga ttatacgttt actgctgaca gcgaaactat
    30241 gaaagtggaa gttgctttta cgtttgacgc tacttctctt gacggtaaac agcttgtaac
    30301 ttttgaagaa ttgtacgatt taagcaatcc agacgagccg aagaaagtta ccgagcataa
    30361 ggatattgag gataagggac agacgattac ctttaaggaa aagccagaag aaccagagaa
    30421 acccgaaaca ccacagatac cagaaaagcc gagcagaccg agcgacagtc ccaaaacggg
    30481 cgacagtaca aatgtaatgg catttcttgt gatgttgctt gcgtctgctg gtggactggc
    30541 tggaacatat ctttacaaac gccgtaaaat gaagaaatca taaggcggta acggtatgat
    30601 gaaagaaaaa tcacgctctc cgccgaagct gtcaaacggc agacttttgc ttattctggc
    30661 ttgtctgcca ccaacctgca agaacacgga tagtcaaggg tgcgaagcat tgattttacc
    30721 ctttactatc tgggggattg ctggtacttc ccaaaccgcc agaataagaa attacaatca
    30781 atttatcaaa atagaaagaa acgaggtaaa acattatgga aatgaaatat gtcgtgccag
    30841 atatggcaca gtcttttgga actcttgaat ttgcaggaga aagcgagcct atctttgaaa
    30901 gagataaaaa taaccgcagg gtcttagcca gacgaagcta taacctttat tctgacgtac
    30961 agaaaggcga aaatgttgta gtggaaattc ccgtgcaggc tggcgaaaag aagttcaagt
    31021 atgaacagaa agtaaaactt gtcaatccga agttgtacgg cagaggttac gcaatcgggg
    31081 atatgggaca taccgattat gtattagttg ctgatgatat tgtagcagtt gaagaaaagt
    31141 aaaggagtga agaatttatg agattatcaa acgggtttgt tattgacaaa gagaaaacat
    31201 ttggcgaatt aaaatttaca gcggtgcgtg atgtgtttat gcagaatgag gacgggacac
    31261 cgagtaccca gcttaaaaag cgtatctatg acttgaaatg tagtctgcat ggtggaatta
    31321 tccccgtgtc cgtaccgcca gaagtgccgt taagggaatt tccgtacaat gcagttgtag
    31381 agcttgtcaa tcccgtagcc gatacggtat caagaaagac gtttggtggt gcagatgtgg
    31441 actggtatgt aaaggcagag gatattgtgt tgaagaataa aggcaatcag aacgcaggca
    31501 atccacagaa ccatgtaccg cagggacaga cgaaaaaata aatctaggtc atagattata
    31561 ctctgaaatt acagtataat gagccaaaag agccgtattt aagctatgta aaataatttt
    31621 gacaaggaaa aaaaggattt gtcgaagata ttggatagac atttgagggt taatatatga
    31681 caatgacacc gaaaggagca atcggaatat ggaaataggt aagaaactta aaaatgcacg
    31741 agtacagtct ggaatgacac aagaaaatgt tgcagaaaaa atcaatgtat caaggcagac
    31801 aatttcaaac tgggaaaatg aaaaatctta cccagacatt atcaatgtaa tagagttgag
    31861 caacctatat tctataagtc ttgacgaatt gctaaagggt gatgaaaaaa tgattgaaca
    31921 tttagaagaa agtacaaatg ttgtaaaaag caaccaaaaa atgatatggg caattattgt
    31981 aaatatcatt gtcgtagcat tgctgatagc attaaatatg tttataccag ataaccgtta
    32041 tttcttagtt ggaatatttt gtcttatggt tatcacttca tcagcattac tttaccagat
    32101 aatcaaaaaa ttttaaggag gatattttgt tatgaatttg aacatagttt tagcggtaat
    32161 ttgtggagca gttgcacttg taggagcgtt ttgtgttgtg tttcagattt atcatatgac
    32221 tgttatagac gcaacagcca gaggattaaa gcaccctaaa ttttggggag tatttacaat
    32281 gagtgggaat aattcaagtg gtttgcttat gtatctaatc ggaagaagaa aataccctat
    32341 tgtaaatatg agtgaaagca actcaaaaga attagaaaaa cgtaaaaaat cagcaggtat
    32401 tggactgtta tttttggcta ttggtgtaat tggcattata tgtgcgacct taatataact
    32461 aaaaatgcag atttatgatt atatcttgca acatttacaa agcagttagt ctatatgatt
    32521 gactgctttt ttcgtaccca gaaaggagtg aatgatttat gcgtaagatt tggaatagag
    32581 gacaccgtat cagagccagc gacaagcacc ttgtctacca tttttccata gggacgcttc
    32641 tgtttttatt cgtggcggtt ctcctgctac tgaatatcaa acagctcatg cgtaccgact
    32701 gggagcattt cagcttgtta gagaacggct tgacgctttc cccttacaac ttcataacca
    32761 tactgatagc gactggtgtt tgtgcattgg tcgcttttct gtattaccgc ttctgttacg
    32821 acagtttcaa gaagctcctg caccgtcaaa agctggcgag aatgatactg gaaaataagt
    32881 ggtatgaagc cgataccgta caagacagcg gtttttttac tgacctgcaa agcagatcaa
    32941 gggaaaaaat cgtctggttt ccaaagattt attatcaaat ggaaaaggga ctgcttcata
    33001 tccgctgtga aattacgctg ggaaaatatc aagaccagct tttacggtta gaggataaat
    33061 tggaaagtgg attatattgt gagctgaccg acaagacctt acatgacggt tatatcgaat
    33121 ataccctgct ttatgatatg atagcgaacc gcattactat tgatgaagta cgggcagaaa
    33181 atggctgtct taaattgatg aaaaatcttg tctgggaata tgacgcactc cctcacgctc
    33241 tgattgctgg tgggactggt ggcggtaaaa cctattttct gctgacgctc attgaagcct
    33301 tactgcatac caacgctgtc ctttacatct tagacccgaa aaatgcggac ttagcagact
    33361 tagggacagt tatggaaaat gtgtatcaca ccaaagaaaa aatgattgat tgcgtcaatg
    33421 ccttttatga gggtatggta cagcgaagtg aggaaatgaa gcgacacccg aactataaga
    33481 cgggcgaaaa ctacgcttat ctgggactgc caccctgctt tcttatcttt gatgaatatg
    33541 tggcgttttt tgaaatgctg gggacaaaag aaagcgtggg cttacttagc cagttaaaga
    33601 aaatcgttat gttaggacgg caagcaggat atttccttat cgttgcctgc cagcgtccag
    33661 acgcaaagta tttctcggac ggtatcagag ataacttcaa tttccgcgtg ggacttgggc
    33721 gtatcagcga attaggttac ggtatgctgt ttggttcaga tgtgaaaaag cagtttttcc
    33781 agaagcgtat caaggggcgt ggctattgtg atgtgggaac aagcgttatc agcgaatttt
    33841 acacgccttt agtcccgaaa ggacatgact ttttgcagac tatcggctct cttgcacaag
    33901 caaggcagga cgggacggcg acgtgcgaag cgaaaggcga cggcacggac tagccgttgc
    33961 tggtgtggcg taagccacgc cagcaggtaa aacccctcgg tatctaacag aggggtacgt
    34021 ttgcaaatag acaatagacg aaaaacatag gaaacataag gcttctggca tgatttccca
    34081 gtaaaaatcg gctgtgaagt cgccttaaaa aacttcacac ttgacagatt gggggtatgg
    34141 ttctgaatga agaacaatgg ataaaagaat tacgggagaa acggattgct tacggtatct
    34201 cacaaggcag gctggcggtg gcttctggta tcacaaggga atatctcaac aagatagaaa
    34261 gcggaaaaat gaagccgtca aaggaacttc tggaaactct gcataaagaa ctggcaaggt
    34321 tcaatccaga agcaccgctt accatgctgt ttgattatgt gaaaattcgt tttcccacgc
    34381 tggatataca gcacatcatc aaagatatat taaaactgaa tatcaactat atgctccatg
    34441 aagattacgg acattacagt tatacggagc attattcttt aggggacatc tttatctata
    34501 cgtcggctga cgaagaaaaa ggtgtccttt tagagttaaa ggggcgtggt tgccgacagt
    34561 ttgagagtta cctgctggca cagcaaagaa gctggtatga ctttctcatg gacgcactca
    34621 tagacggtgg cgtgatgaag cgtatcgacc ttgctatcaa cgaccatacg ggcattttgg
    34681 atattccaga gcttgcggaa aaatgcagaa aacgggaata tatcggaaag tccagaagtt
    34741 ataagtttta ccagtcgggc gagcttatca agcacagcga ggacgacaga gaatatatgg
    34801 gacgtaccct ttatcttggt tcgctgaaat ctgatgtata tttctgtatc tatgaaaagg
    34861 actatgagca gtatgtcaag ttagggacac ctctggaaga agccgatatt atcaaccgtt
    34921 ttgagatacg gcttagaaat gaacgtgcct attatgcagt acgggatttg ctgacgtatt
    34981 acgacgcaga gcagaccgcc ttttctatca tcaatcagta tgtacggttt gttgatgaag
    35041 aaccagacaa gcggaaaaat gactggaaac tcaatgaccg ctgggcttgg tttatcggcg
    35101 ataatcggca aagtctgaaa ctgacgacaa agccagaacc ttatacctta gatcgtacat
    35161 tgcgttgggt acaaaggcag gtagcaccga ccttgaaaat gctgaaaaag attgataagg
    35221 gaaacggtac agattacatg gagacaatcg aacagcaggc aaagctcaca gaaaagcatg
    35281 aaatgataat caaacagcag acgacccctg caaaagattt agtagaaatt taggagtgat
    35341 gaaaaatgtg ggtattatta aaagacttcc tgctggtatc tatgggaatg ggtatcggca
    35401 tagtcttgat gtgtattttg aatgtcggca aagaagctga ctataaaatg aaacaattag
    35461 aagaaagcga ggacaattaa atgaatttcg gacaaaattt gtatcaatgg tttttaagta
    35521 acgcacagag cttagtgctt atggcgattg tggttatcgg tatctactta gggttcaagc
    35581 gtgagttttc caaacttatc ggcttcttgg tagttgcctt gattgctgtc ggtttggtat
    35641 tcaatgctgg cggtgtgaaa gatgtattgt tagagctgtt caataagatt atcggtgcat
    35701 aaaattttat tgtggtactg atatgcgaat gttataattt gaatgttgta ttacataaaa
    35761 cgctgtactt accagaaaaa tctatgaatt ttgtatatta tacaaggttg caacttgaag
    35821 ttgctaatgt gcatttcaaa aaatatagaa tataacatta tggcttgata aaatttattc
    35881 atcaaatcat aggaggtatc acacaatgag tttaggagaa aaaatcttca agttaagaaa
    35941 agaaaaagga atatcgcaag aagcactagc tgaacaaata ggcactacta ggcaggcaat
    36001 tagtaaatgg gaaaacaatc aagggtttcc agaaacagaa aagcttttac aattatcaaa
    36061 catttttgaa gtatctacgg attatctttt gaaagacgaa aagaatgtaa agaatagtga
    36121 agaacgagga tattatgtaa gcaaggaaat gtcggtaggg tttatcgcta atcagaaaaa
    36181 attaggactt tatcttgctt taggggttat gctttgcatt tttgctggaa taccatatac
    36241 cttgttttta gataatatga cttggcgtta tttaggtatg gcattttgct ttgtggcagg
    36301 aattatattt tttgtcgtag caatttttta tgaaaaacaa gaatacaaca ttttaaggca
    36361 agaaccttta ctacttgatt atgaatatct aaaagaattg aaaagcgaat acacttcaaa
    36421 aaagaaaaaa tatgtaatag ttgctgttcc ttgtactgtt ctctttatag caggactaat
    36481 tttctttcta tttactgtga aaggctatat ttcatggaca gaatatcatt cctttgcatt
    36541 tttcggtctt gctgttggaa tgttgggttt tgtatataca atcaatgtta tggaaaccta
    36601 tgaactatta gtgaaaaacg agcaatattc aacgagtttc tttttcaaac taaaaagaaa
    36661 gattaaaaag agattaggat aagctataat ttaatattca cacaaagcag ttagtcttat
    36721 gattgactgc ttttttcata ccaaaatttc agattggagt gattacatga acgatatttt
    36781 taaggatatg caggcaaaag tcggctgtga atacatttct gacctgccct cttacaagcg
    36841 taaggtgtgg cgtgaaatga aacggctgag ccctgccgat tatgaagaaa gacaattaga
    36901 agatttttca aagtttgtgt ttggtatgcc gtaccagacc ttaaaagatg tgatgaaaca
    36961 acagaaagga cgtgaagaac aatgcaggaa acaagggtgc tggtggaaac gagagaaaca
    37021 actggcgaag aaacaacatc atactggttc gacctgccga tagatgttgc cgagtttgaa
    37081 gaaaagttag gtgtcggtgc agaaagcacg gcgtaccgca ttatcgaaaa ggtgttgcct
    37141 tatgctgatg aagtccacga acatacaagc gtgtaccagc ttaacgaatt agagtttatg
    37201 taccgccagc tttcaagcga tatgcaggaa gaatatgtat cactacttac ggtgtttgag
    37261 aatttagaag cactttatat ttgcaggaac gtgattacgg tttatcccga ctgcaaaagc
    37321 atgatagatg ttgcaaggca aaagctgatg aatgactcga cgttcaaaca tttatccgag
    37381 gactgtcaag aatactactt tgactttgaa gcctacgctt ctcacttgca ggaacacggg
    37441 aaattcttag taacggaaca cggtatcttt gaactaccag agtaggaaat gaggtggtgc
    37501 ttatgataga tgatatggcg gtttacattg ctaatctcgg taaatacaat gagggttatt
    37561 tagtcggtgc ttggttcacg ttccccattg acgaggaaga tgtaaaagaa aaaatcggct
    37621 tgaatgaaca gtatgaggaa tacgctatcc atgataccga caacttcccc attgcgattg
    37681 gcgagtatgt ttccattgaa gaactcaatg agatgtatga aatgatagag gaacttcccg
    37741 actatatcgt agagtgtctg gacgaattta tcagtcacta cgggacgctg gaagaagtcg
    37801 tggaacacaa ggacgatatt tattattatc ccgactgtga aaccatgaca gatgttgcct
    37861 attactacat agacgaattg caggcacttg gggacattcc acccagctta cagaactaca
    37921 ttgattatga agcctacggg cgagatttgg atatgggcgg ttgctttatt gaaacaagcc
    37981 gagggatatg cgagatacca tattaacgct ggaagatcag caacggcatg gttgctactg
    38041 acagcgtatt tctcttttaa gggacagtct gaaaatggct gtccttttct ctttgaagaa
    38101 cttacgaaag gagctgaaag aacttgaaaa agattaaatc ttatacgggt atctggaacg
    38161 tggaaaaagt cttgtatgca atcaatgact ttaacctgcc ttttcccgtt acctttacac
    38221 agattacatg gtttgtgatt acggaattta tcatcattct gtttggggat attcccccac
    38281 tttccatgat tgagggagca tttctcaaat acttcggtat tcccgttgct ctcacttggt
    38341 ttatgtcgca gaaaaccttt gacggaaaga agccgtacag ctttttgaaa tcacagataa
    38401 cctatgccct gcgacctaaa atcacttatg cgggaaaagc cgtaaaactg cataagcaga
    38461 ccttgaatga aacaatcacg gcagtaagga gtgtgaacta tgttcccgat aaaatatatt
    38521 gacaataacc ttgtctggaa caaggacaat gaggtgttcg cttactatga gctgataccg
    38581 tacaactatt cttttctatc cgcagagcag aaatttatcg tgcatgacag tttccgacag
    38641 cttatcgcac agtcccgtga gggtaaaatt catgcgttgc agattgctac ggaaagctca
    38701 atccatagta tgcaggagca gtcaaagaag ctggtaactg gaaaattaaa ggaagttgcc
    38761 tgccagaaaa tagacgaaca gaccgaagcg ttagtatcta tgattggtga caatcaagtg
    38821 gactaccgct tttttcttgg ctttaagctc atggttacgg aagaacagct caatctgaag
    38881 aacataaaaa aatcggcgtg gctgacgttc acggaatttc tccatgaagt gaaccacacg
    38941 ctgatgaatg attttgtttc catgccgaat gatgaaatca accgttacat gaaaatggaa
    39001 aagttactgg aaaataaaat ctcccgtcgc ttcaaggtgc gtcgcttgga aatcaatgat
    39061 tttgggtatc tcatggaaca tctttacggc aggaacggta tcgcctatga agattatgag
    39121 tatcagttac caaagaagaa attgaacaaa gaaacgctga taaaatacta cgaccttatc
    39181 cgtccgacaa ggtgtgtgat tgaggaaagc cagcggtatt tacgattgga acatgaggac
    39241 aaggaaagct atgtgtccta ctttaccgtc aatgcgattg tcggggagct tgattttccg
    39301 tcaagtgaaa tcttctattt ccaacaacag caattcacat tccccgttga tacttctatg
    39361 aatgtagaaa tcgtggagaa tcgaaaggct cttaccacag taagaaataa gaaaaaggaa
    39421 ttgaaagatt tggataatca cgcctatcaa gcaggaagtg aaaccagttc aaatgtggtg
    39481 gacgcattag acagcgtgga tgagctggaa acggacttag accagacaaa agaaagtatg
    39541 tataagctct cttatgtagt gcgtgtatcg gctgatgatt tggacgaatt aaaacgccgt
    39601 tgtgatgaag tcaaggactt ttacgacgac ctcaatgtaa agctggtgcg tcctgctggg
    39661 gatatgctgg ggcttcattc tgaattttta cctgccagca agcgatatat caatgattat
    39721 gtgcagtatg taaaatcaga ttttttagct gggcttggct ttggtgcgac acagcagtta
    39781 ggagaaacta cgggtatcta tatgggctat tccgttgata cgggaagaaa tgtgtacctg
    39841 caaccgtccc ttgccagtca aggtataaaa ggtacagtta ccaacgccct tgcgtctgct
    39901 tttgtcggtt cgcttggcgg tggaaagtcg ttctgtaaca atcttctggt gtattattcg
    39961 gttctctttg ggggacaggc ggttatctta gacccaaaat cagagcgtgg caactggaag
    40021 gaaacacttc cagaaatcgc acatgaaatc aatatcgtaa acttaaccag cgataaagga
    40081 aatgcaggac ttcttgaccc attcgtgatt atgaagaatg taaaagacgc tgaaagtctg
    40141 gcaatcgaca tcttaacatt ccttacgggt atttcctcta gggacggcga aaaattcccc
    40201 gtacttcgta aagcggtacg ctccgttacc cagagcaaca gtcggggctt gctccatgtg
    40261 atagacgagc tacgccgtga agatacgccc atatcaagaa atatcgcaga ccatatcgac
    40321 agcttcacgg actacgactt tgcacatctg ctgttttcag acgggacggt agaaaatgct
    40381 atcagtctgg ataaccagct caacatcatt caagtagctg accttgtact gccagataaa
    40441 gataccactt ttgaggaata cacgaccatt gaattattat cggtttctat gctgattgtg
    40501 attagtacct ttgcccttga ttttatccat tcggacagaa gtatttttaa gattgtagac
    40561 cttgacgaag cgtgggcgtt cttaaatgtg gcacaaggag aaacgctctc taacaagctg
    40621 gttcgtgctg gacgagctat gcaggcaggc gtttattttg ttacccagtc tgcctatgat
    40681 gtttcaaagg aaagtctgaa aaacaatatc ggcttgaagt ttgcctttcg ttctactgac
    40741 attaacgaga taaaacagac cttagagttt tttggtatcg acaaggacga cgagaacaac
    40801 cagaaacgct tgcgtgattt agagaacgga caatgcttat tgcaggactt atacggacgt
    40861 gtcggtgtgg tgcagataca ccccgtcttt gaagaactgc tacacgcctt tgataccaga
    40921 ccgcccgtac agagaaatga ggtggagtga tgaaagaaag gataaaaggt gcgttcacaa
    40981 aaaagaagat tttccacttt ctcaaaatgg ttctgttcgt ggtggcactc tcccttatcc
    41041 tgctttcact tctggggacg gtggctcatg cgacggggct tgtggacgat accataaatg
    41101 cagaaaatct ttactcaaaa tatccgctgt cgaattacca gcttgatttt tatgtggata
    41161 atagctggtc gtggcttccg tggaactggc tggacggtat cggaaaatcg gtgcagtatg
    41221 ggctttactg cattaccaac tttgtctgga cgataagcct ttatttaagc aatgccacgg
    41281 gctatgtggt gcaggaagcc tataaactgg acttcattaa cgatatggca gacagtatcg
    41341 gaaaaagcat acagaccatt gcaggcgtaa cacagaacgg tttttcttct acgggcttct
    41401 atgttggttt cctgcttctc attatcttag tggtgggact ttatgttgcc tatacgggac
    41461 tgataaaacg ggaaaccagc aaggcacttc atgccgttat taactttgtg gtggtgttcg
    41521 tgctgtccgc ttcgtttatt gcctacgctc ccgactacat caaaaagata aatgaatttt
    41581 caagtgatat tagtaccgct tcgttggact tgggaacaaa aatcatgctc ccaaactctg
    41641 acagcaaggg caaggacagc gtggacttga taagggacag cctattttct attcaagtgg
    41701 aacagccgtg gttactctta caattcggga acagcaacgc agaagaaatc ggggcagacc
    41761 gtgtagaagc tctggtatcg gcaagcccag aggacgagga cggaaagacc agagaggaag
    41821 tcgtgaaaac agaaatcgag gacaacgaca acaacaatct gacgataccg caggtcgtga
    41881 accgtttagg tatggtgttc ttcctactgt tcttcaactt agggataacg atatttgtat
    41941 tcttgcttac gggcatgatg ttattcagcc agatactttt tattatcttt gctatgtttc
    42001 tgcctatcag ctttttactt tccatgatac cgagctatga aagcatggca aagcaggcaa
    42061 tcgtgagggt gtttaatacc ataatgacac gagcaggaat aacgctcatt gtaacggtgg
    42121 cgttcagcat ttccagtatg ttttataaca tatccacgga ctatccgttt ttcatggtgg
    42181 cgttcttaca gatagtgtgt ttcgctggta tctacatgaa gctgggtgac ttaatgagta
    42241 tgttctcttt gaacgctaat gatagtcaaa gcatgggacg aagaattttc cgcagaccat
    42301 atctatactt ggcacatagg gctagacgta tggaaagacg gcttgctggt gcggttactg
    42361 ctggtggtgt tgctggtgcg gtggctggta gcacgatttc aaacagaaaa gcgacaagga
    42421 acacagctac aaaagatagt cggggtaata catcttccag catgggacag cgtgtaggtt
    42481 caaaggtggg tgctgtctta gatacaaaaa ataaagtgaa agataaggca agtgctgtca
    42541 aagagaatat caaggatatg ccgacacaga ccgcttatgc cgtccactcc gcaaaggaaa
    42601 aggcaaagtc cagcgtgtcc gacttcaagc gtggcatggt gcaggaacag cagtccagac
    42661 agtcgggacg tatggaaaaa cgggaacagc ataggcagaa tatcgccgat aagcgtatgg
    42721 agcttcaaaa ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa
    42781 cgggagctac ccgtccccat gagcgaccag ccacagcttc aaccattcca aagccgagtg
    42841 ctgaaaaaac gcaggaagtc aaacgccctg ccacagcgac gacttcaaaa acaaatgagc
    42901 cagtcaagac aacggttatc aaagagcgtc ccttatcttc gggtacttct gataggaaaa
    42961 cgagccagcc tacacagcca gtacataggc agaatgtaga aaaagtggta tcgcaggaaa
    43021 cacgccagaa tgacaccaaa gaccgcagga caaaagttca gcagacgcaa agcgtccaga
    43081 aaaaccagca gaccacagag aaaacccgta accttgtaat gaagaaagga cagaagaaaa
    43141 aatgaaactg aaacatattg ctattatcgg cagtctgttt cctatcctct tttcactggt
    43201 gcttttcttt ggggtgttga ttagtgcgga cagcgacgac gagaacagca acttttcttc
    43261 tggcattacg ggtatgaact tatccgcaga agtcttgaaa catcagccta tggtggaaaa
    43321 gtacgccaga gaatacggca tttccgagta tgtcaatgtg ttgctggcaa tcatacaagt
    43381 ggaaagtggc ggtacagccg aggacgttat gcaaagctct gaaagtctgg gactaccgcc
    43441 caattcatta gatacagaaa gttcaatcaa gcaggggtgt aaatattttg cgtccctgct
    43501 ttcgtcctgc aaaaatcaag gtatggacga tttgaatgta gcgatacagt cttataacta
    43561 tggcggtggt tatgtgggat atgtggcagg aaaaggaaat aaacacactt tcaaccttgc
    43621 agagagcttt gcccgtgaaa aatcgggtgg aaagaaagta acatacacca atccaatagc
    43681 tgttgcgaag aatggaggct ggcggtatag ctatggaaat atgttctatg tggaattagt
    43741 caatcagtat ttaactgttc cgcaggtgtc gggagagctg gcacaaaagg taatgaatga
    43801 agcgttgaag tatcaaggct ggaagtacgt ctatggtggt agtaatccca acacttcctt
    43861 tgactgttcg ggactgacac aatggtgtta tggaaaagct ggtatctcct taccgagaac
    43921 ggcacaagca cagtatgatg ctacccaaca tctcccactc tcgcaggcaa aggctgggga
    43981 cttggtattt ttccattcta cctataacgc tggttcgtat gtaacccacg tcggcattta
    44041 tgtaggaaat aaccagatgt accatgcagg cgacccgata ggatttgcag acctaagcag
    44101 tagttactgg caacagcact taatcggtgc aggacgagta aaacaataga aaggacttga
    44161 agatatgttt aagaaaaata agaaacagac agaaactatc aaagaaccaa aagaaaaaaa
    44221 ggtgcgtact gtcaaggtag gcacacataa gaaaactgtg attgccttgt gggtggtgct
    44281 tatcgcaagc gtgagcttcg gggtgtataa gaattttacc gctatcgacc agcacaccac
    44341 ccatgaaaaa gaaatcattg agcttcgctt gcaggacacc aacgggatag aaaatttcgt
    44401 gaagaacttt gcgaagtcat attacacatg gaataacagc aaagaagcga ttgaagcaag
    44461 gacgcaggca atcagcggtt atctgacaaa ggaattgcag gacttgaata tagataccat
    44521 tagaaccgac ataccgacca gctccacagt tacagatgtg attgtatggc atatcgagca
    44581 atcgggaacg gatacttttt ctgctaccta cgaagtagat cagcagataa aagagggcga
    44641 acagacaagc aatgtgaaag caacctatac tgtaaaagtt catgtagacg ctgacgggga
    44701 tatggtaatc gttcagaacc ctactcttgc accagcaatc gaaaaatcag actatgatcc
    44761 aaagacacca gaaacagacg caagtgtaga cgctgatact gcaaatgacg ctaccgcatt
    44821 tctggaaaca ttctttaagt tgtacccaac agccacagaa aacgaacttg cttattatgt
    44881 gtcggggaat gtgcttgaac ctatcggcag ggactacctt tattctgaac tggtaaaccc
    44941 tatctttaca aaagacgggg agaatgtaaa agttaaggtt gctgtaaaat tccttgataa
    45001 tcagacaaag gcaacgcaag tatcgcagta tgagcttgtg ctacataagg atagtaactg
    45061 gaagattgta ggataattgt aaaaggcttg tatcatattt ttatgctctg caagcctttt
    45121 aatattgaca actgtatttt taagtattat gctgtacttg tataagctat acagtataga
    45181 acaaggcggt gtttaaatgg aaatctttat caataatcat gcaagcatac ctatttatga
    45241 acaaatttct tcgcaaataa aagcattgat tataaatggg gaattaaagg ctggcgaagc
    45301 tctaccatca ataagaggac ttgcaaaatc attgcatata agtgtattga ctgttcaaaa
    45361 atcttatgat gtgcttcaaa aagacggttt tatcgaaaca acagcaggca aagggtgttt
    45421 tgtatctgct caaaatcaag atttttatat agaagaacaa cagaaaaaaa tcgaagatag
    45481 atttgtagaa gctattgaaa tagcccgtgc aagtggcata aaccttgata aattgattgg
    45541 attattaaaa ttattatatg aggaggacga gtaaatggct gaaaatattt tagaagttaa
    45601 aaaccttaca aaagattatg gcgattttgt attaaatgac ttaagttttt ccattcctag
    45661 aggtgtaatt atggggctta ttggcgaaaa tggagctgga aaatcgacca cagtaaattg
    45721 tattttgaac gaaattaaga aaaacagtgg aaacattttg atttttggaa aagaccacat
    45781 aattgatgaa tgtgaaataa aagataaaat cggaattgta tttgacgaaa accactttcc
    45841 agatattttt acaccaaaag aaattgaaaa atatatgtcg ggaatatata ccaattggga
    45901 cagtatagca tttcgtaatt atttgaaaca gttcgaatta ccagcaaaga agaaaattaa
    45961 agatttttct aaaggaatga aagtaaaact tgcattcgct gttgctcttt cacataaagc
    46021 agaattactt atattagatg aagcaacaag tggattggac cctattattc gagatgatat
    46081 tcttgatata ctgattgatt ttgtgcaaga tgaaaattac tctattttgt tttcatcaca
    46141 tattacaagt gatttagaaa aaatagcaga ttatattact tttattcata aaggacggtt
    46201 agttttctgc tcattaaaag acgacttaat tgataactat ggtgttgtaa gttgtggagc
    46261 aagtgtcttt gatacgctag ataagacaga aattatttcc tatcaaaaag aagattatca
    46321 atataaggtt cttgttcgtg atagaaacaa gattgcaaaa gattttccaa aagcacttgt
    46381 agaacctgct actattgaaa atattatgtt gttttacgta aagggggaat tacaatgaaa
    46441 ggcttattga tgaaagattt attagtaatt cagaaaaaat atggaattac tagaattatt
    46501 atggatatat tcattatagt agctttgatg ttatttttaa aagggacagg agctatttac
    46561 attagtttat tgttaattcc agttgaaata atgacaatga taatttcctt aacgacttgc
    46621 gacgaacaat ggaaatgggg gaaatatgtt gttgcactac ccgtttcaaa aactacgatt
    46681 gtaaaaagca gatatatagt tgctttgatt atgacttgta ttggctttgg ggtagctttg
    46741 cttgttaata ccataacata ttttatattc cctcaatttg catttggtta ttatatgttt
    46801 attgcttgtg cttcttttgc cgtaacctta gtatttttgt catttgtatt gccttcaaat
    46861 tattcacttg gagttaatgc aggttttgcg gttatgttta ctctagtgat tattcttgtt
    46921 gtattggcag tttggacaaa tgtgactaac aattctattt tatggtttgt agtcgataat
    46981 tttgaattga gtatcggcat agcttttata atagttatag ctcttgcttt cagttcttat
    47041 cttctatcaa aagcattttt tactagaaaa tacaattagt gaggtattgt tatgagaaaa
    47101 tgtcttagat gtcatgaaga aatggtcgaa aatttagatg ttaaagttga tatgcaggga
    47161 tatggtatta gaataactac taaaggcgtt tttggaacta cggtagaaaa acctaaagtt
    47221 gcagtttgtc caaaatgtgg cgaagtttcc ttatatattg aaaattttaa atcaataaaa
    47281 taaatgatta aaatctgcat atagactgag ggtttattgt ttgctaacgt ccgagcggat
    47341 attgaagaat ttatgaatag tacataccca gagatgcaag caaatttcaa aaggtatttt
    47401 ggaaatagac attttacgcc gaaaaaatat gtttatatca ctacgaaaaa gcaaaaatta
    47461 aatttcttaa aagaataagg gggggctatg gataagcaat tatgggtatt atgtcctatt
    47521 tgcaataata agacacgaac taaagtgcga gaagatacag aactgataaa ttttcctttg
    47581 ttctgtccga agtgcaaaac ggaaacttta atcaatataa agcatggaaa agtaactatt
    47641 atcaaagagc cagacgcaaa gacgcagagc cgataatttg taggttatta tgacttatag
    47701 attgttggct cttatttgtt acctgcggag cagatcagca aatagataat gcagaatagc
    47761 aaaaagggaa aacaagaagc tgattacttc ttgctctccc tgctgttcaa gataccgtca
    47821 atcgtacctt gaatgatttt taattcgtta tcactcaata aatcaagaga ggtttctatc
    47881 tgacggcgag ctgtactttt tacgacgttc tctgctggat agaaatattc atcgaccgat
    47941 atattgaaca tggtaactaa atcatggaat aattgaaaac tagggtgtga accaacattt
    48001 tcaatatctg cgatatggcg ttctccatag aacactttat cgcctaaatc attacgagaa
    48061 agccctgctc tctcacgggc ttctctgatt gccagtccta aaggtctgaa atcaaaagtt
    48121 tgtgtttctt ttgcttttct cattttaaat cacctagcat gagtttatac ttcatgctgt
    48181 atatttagaa tgtgttaaaa ttgagcgaat cattcaatta gaaaacgaaa aagaatagaa
    48241 tcacaaatca cttgtgattt gtgattgttg atgataaaat aagagtaaga agaaatagaa
    48301 agaagtgagt ggttgtggga aatttaggcg cacaaaaagc aaaacgaaat gatacaccaa
    48361 tcagtgcaaa aaaagatatt atgggggata agacggttcg tgttcgtgct gacttgcacc
    48421 atattataaa aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt atggataaag
    48481 ccttagaaga atatattcgg aaatatttac ctgacaaact ttaaaaggag tgaaaatgaa
    48541 atggcagtta cttatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc
    48601 gtttataatc gagtattaaa ctatgttttg aatcatgaat taaataaaaa tgactctcaa
    48661 ttattggaag tcaatttatt aaaccaatta aagcttgcaa aacgtgtaaa tctttttgat
    48721 tattctttag aagaattaca agccgttcat gagtattggc ggtcaatgaa tcgctattca
    48781 aaacaagttt tgaataaaga gaaagtggct taatatggca aatatagtca attttactga
    48841 caaacaattt gaaaatcgct taaatgataa tttagaagaa ttggttcaag gaaaaaaagc
    48901 ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca gggtcaggga aaacgagttt
    48961 gcgaagaaga tgattgagca ggcaggaaca aagtgataaa ttcaaattgt gagggaaaat
    49021 atagatgaat caaggaagaa ttattgtgat tacaggctca ccggggacag gaaaaacaac
    49081 aactgcgtca attgtcgcaa aggaatcaga tatggataaa tcagtgcata tgcacaccga
    49141 tgactttttt cattacttaa gcaaaggcgc aataccacca catttgccag aatccaacaa
    49201 gcaaaatctg gttgtcattg aagccttttt agaggctgca aagcgatatg ctcgtggcgg
    49261 atatgatgta attgtagatg ggattgtcgg gccatggttt ttggagccat ggaaagctct
    49321 tgcccaagaa gattacgagg tacactatat tgtattaaga gcgagtatgg aaataagact
    49381 tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt ataataggaa
    49441 ttgaagttaa attagatgct aaaaatttgt aattaagaag gagtgattac atgaacaaaa
    49501 atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac
    49561 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
    49621 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
    49681 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
    49741 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggagt attccttacc
    49801 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc
    49861 tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
    49921 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
    49981 ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
    50041 ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc
    50101 aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa
    50161 gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
    50221 ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat
    50281 gtagataaat tattaggtat actactgaca gcttccaagg agctaaagag gtccctagcg
    50341 cctacgggga atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt
    50401 gtaggaaaat gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt
    50461 tcaaattatc ctgaatgtac cttcacttta gctgaacatt ttagaaagaa aaaactcacc
    50521 aaaacaaatg taaaagaatt actagaggga aaagaaaccc tggtaaaagg aatcaaaacg
    50581 aaagatagaa agtcctacaa tgccgttgta aaaatcggag aaaagggata tattgatttt
    50641 atctcttttt caaaataagc ataaaagccc tttaaagagg gcttttatat attaatcaca
    50701 aatcacttat cacaaatcac aagtgattaa tcacaaatca cttgtgattt gtgattccta
    50761 atgatacaat attactatac aaaaaaagaa tggggcgtag ttatggagaa ggaagaactc
    50821 aaaatacttg aagaattaag acgtatttta aacagtaaaa atgaagcgat tgttatctta
    50881 aacaattatt ttaaaggtgg tgttggaaag tcaaaattat cgactatgtt tgcttacttg
    50941 acagacaaat ttaatttaaa agttttaatg atcgataagg acttacaagc aacattgaca
    51001 aaagacttag caaaaacatt taaggtagaa ttgccacgtg ttaattttta tgaaggactg
    51061 aaaaatggaa acttggcttc ttctattgtt catttgactg ataatttaga cttgatccct
    51121 ggcacgtttg atttgatgtt actgccaaaa ttaactcgct catggacttt tgaaaatgaa
    51181 agtagattgc ttgctactct tttagcacct ttaaaaagtg actatgatct cattattatt
    51241 gatactgtac caacgccaag cgtttataca aataatgcaa tcgtggcaag tgattacgtc
    51301 atgatccctt tacaagcaga agaagaaagt acaaacaaca ttcaaaacta tatttcctat
    51361 ttgattgatt tacaagaaca gtttaaccct ggactagata tgatcggttt tgttccttat
    51421 ttagttgata cggacagcac aacgataaaa tcaaacctgg aagaactgta caagcaacat
    51481 aaagaagata acttggtttt ccaaaatatt atcaagcgaa gtaataaagt aagtacctgg
    51541 tctaaaaacg gcattacaga acacaaaggc tatgacaaaa aagttttatc tatgtatgag
    51601 aacgtatttt ttgaaatgct tgcatatagt atgcagtaat aattcaaaag ctgttatcgt
    51661 gtgcggtaaa taaaaaaaac cgttcctaga taatacactt ttgtgcgtct tgaaaaataa
    51721 cgggacggtt ttggatataa ccagaactgt ccttttttct taggagtaaa taaggcatag
    51781 ctctggttaa agggggctat accatgattg catacggaag aacgttctgc cgttctcaac
    51841 tgaataaata tttcagtcca ctattacaat cattacaccc tttacaagaa ttaaaaattc
    51901 atatcaatag aatttataaa acagtctgtt tgtgtaatta cacattatcg ggctgttttt
    51961 gttttgttaa aaattttttt caactttttt cggtttcaac ttagcaaatc acacctgtcg
    52021 ttccctatat ggtgcggaaa gagggataac cacttttcat atcgtaacgg aaagggggtg
    52081 agattgatga aaccatctga cttccagaaa acagttcaat gccgttttga aagttgtttg
    52141 aagaaagttg tccgacatgt tgttaaggac tatcagaaga aattaaagcg acgacaaaag
    52201 ggagaaacgc ttttttgtga acttccagaa atcgttgtag aaaatctggc tgtctgggac
    52261 gactatgata cggactatac gattttcaat gtatgtggta gtgatattcg tatttgtgat
    52321 gatgaactgg cagaagcctt gaaacatttg tctgagcgta atcgagaaaa tttgttgatg
    52381 tattatttct tggaaatgag cgataccgag attgcaaaaa gacaaaatat ttcaagaagt
    52441 ggtgtttttc aaaaccgaca caattcacta gagcttatga aaaaaatact aaaggagaaa
    52501 tgatagcaaa tgaaacacac aatgaagaag ccgtcctact acttgctttc gcaggcaatg
    52561 gacggggacg aaaaagcgat tgaaaagata ctggcatttt atgaccctta catatcaaag
    52621 tgctgtctac gaccactcta tgatgaatac ggcaatgtct atattgttgt agatatggaa
    52681 ttaaagggac ttatcagaga agcacttatt aaaatgattt tagggtttga tattgcctta
    52741 gaaatcgaag aagaataaca agcagtaacc gcagagcatg atttgttcaa tcccctcttt
    52801 cctgctctgc ccctgctttt atatgaaagc aacacgcttt cgccacagct ctttgacaac
    52861 tgaataaagc agacagatac gtttgtgaga tagcgagcca cgggaactgt acgccacgac
    52921 cttttcaaaa gaaagcgagc gacccacgca gtgatccgag agcgactgtt ggaaaagtcg
    52981 ttttgccatg acctatcctc acagaataat gatacgtccg catggtgcgg ttctgcccac
    53041 aagaatggga atagttgaga tactaacgga gctttccaaa gccgtctgcc tgcttataat
    53101 tgatagtttc agcaacaaaa tctatctact ctttttattt aggtgtgtta taatgacctt
    53161 attagacgag tgtattttgt tgctgtcaca aatgaaaggg gtcttaatag tgataagtga
    53221 tacacaaaat gcacaatctc ctacaaagga aaaaaagact tatacagtag aggaaattgc
    53281 tgaacaactg gacattagca aaaaggtagc ttattcatta gtaaaaagcg gtcaattcag
    53341 ttatgttcgt gctggtaaag ccatacgggt atcaaaggtt tctttcgata aatggttaaa
    53401 tgaaatttaa ctataaggag agaaaattat ggcttcaatc aaacaaagaa aatcaagttt
    53461 ttcagttatt tattggtact tagatagtgc aggagaaaga aaacaaaaat gggacacgct
    53521 ggaaacaaga aaagaagcaa aacagcgaaa agcctttata gaatactatc aagagaaatt
    53581 tggatatgtg atagttccat tagaggaaca atttgcacat cagattgaaa attctaaaaa
    53641 ggagttagat gttgtagaca aagatattac attgcgtgat ttcctagaaa tttttgttaa
    53701 tttatatggc acatctaaat ggtcgccgag tacatttagt agtaaggtag gcagtattga
    53761 aaattatatc aatccgatta ttggagattg gaaactgaat gaaataacta caaaaagtct
    53821 atcaaaatac tacaatgatt tattgagcgt tccagaagta ccacgggcaa acagaaaagc
    53881 aactggtcgt tgtgttcaac cagcgaacat caaaaaaata catgatatta tccgctgtgc
    53941 cttaaatcaa gctatccgct gggagtatat tgatacaaac aaaagaaacc ctgcgtcttt
    54001 ggctacgtta ccaaaaattc caaagactag gcgaaaagtg tggagtgttc aaacatttcg
    54061 agaagcggta aaagccacgg aagatgattt gctttcaatt tgtatgcact tagctttttc
    54121 ctgctctatg cgaattggag aaattacagg acttacatgg gaagatgtta ttattgatga
    54181 agagtccatt gctacaaata atgcaaaggt aatcatcaat aaagaacttt caagagtaaa
    54241 tgcagaagct atgcagaaat taaaggaaaa ggatatttta aagattttcc caacgcaaaa
    54301 accgcattgt accacaaggc ttgtcttgaa aacgccaaaa acagaaacaa gtaatcgtgt
    54361 tgtctggtta cctaaaacag ttgcggaact attggtgcaa tataaaaaag accagcagga
    54421 attaaaggaa tttttaggaa gtgcctacaa tgattacaac cttgttattg cgttggataa
    54481 tggcaatccc gtggaaagcc gtattgttag agataggttt caaaagctct gtgaagaaaa
    54541 cgattatgaa gttgtggtat ttcacagcct acgccattta agtacgggtt ataaactcaa
    54601 aatgacaaac ggagatgtta agtccgtaca aggcgatact ggtcacgcag aagcagaaat
    54661 ggttacagat gtatattcag aaatcattga cgaggacaga cgtttcaatg cacaaaaaat
    54721 ggataaagaa ttttattcta cgcttaatga tgatacagac aatccaaagc aagataccga
    54781 cttaaccgat agtgatatgg cgttattgga actgattaaa tctttatcgc cagaaatgaa
    54841 agagcaattt ttaaagcagg cacttcaaag atagaatttc aaaagtgtta gcaaattcca
    54901 aaaaaaccag cgatttttct tcaaaaataa aaaagtgtta gcaagcctat ttccttaaaa
    54961 gtcttaaaaa aatatcgaat aaaaaatgac agattgaaaa caattcgtat tcagcgtaaa
    55021 tagaaagcca taaataaaga aacgctgaaa accttgtaaa ataaggcttt cagcggagtt
    55081 tgtggcgtcc cagacaggag tcgaacctgc gacgtttcgc ttaggaggcg aaccctctat
    55141 cctgctgagg tactgggaca ttcttaaaaa tctattcaat tttatctagt tatcatatca
    55201 aaaggcgttt gttccaccta gcccttagga ggtctccatt cagcagcgaa caagttacct
    55261 ttaccattag gagtagaaaa cttttgacct tcatacagat acatagttcc tttatcttcc
    55321 atactttcac tcttacaagg ccattgaaca cacccttgag tttctatctt ttcataagta
    55381 gcacctaaga aacttggaca taattgtcta agttcatccc atatttcctt agtattttta
    55441 taattcatag gatatcccat tgctgtagaa atttcgctta taatttgcca gtctggtttt
    55501 acatcccctt gtggctctat agcttttctc atcaattgga atcctctatc tgcacatgta
    55561 tatactccct catgttctcc ccaagaagta gctggaagta taacatcagc gtgaagtgct
    55621 gtcttattca taaatatatc ttgaacaatg acaaattcca attcatctaa tgcttctcta
    55681 acttctgatg catctgggtc actttgcact gggtcttctc caaatatata ataagcttta
    55741 agctttttct cttttagtac taagtctgga acttgagtta aactataacc attattagga
    55801 gatagtttta ctccccaagc tttttcaaac ttttctctta tcttatcatc agttacattt
    55861 tgatagccag gatatacatt aggaagagct cccatatcac aagctccttg aacattattt
    55921 tgtccacgta caggtcctat tccaacgctt tctctaccaa aattacctgt aagtagtgca
    55981 attgatgcaa gtcccttaac tacatcaaca gcttgaccaa attgacagac acccattcca
    56041 taaagtatca tagctttttt accttttgca tattctctca tagcttttct tataagctct
    56101 tctgggattc ctgtaatctt ttcagcatat ttagctgtat atggtttaac aatttcctta
    56161 tactcatcaa atccttgtgt atgattttga acaaattctt tattgtaaag accttcttct
    56221 attaatacat ttccaaaagc atttactaaa accatatttg ttccaccttt tataggtaga
    56281 tgtatatcag caattcttgc agattcagtt actctagggt cagtaacaat taatttagca
    56341 ccattttttt tagcttttac tattctattt gccactattg gatgtgaatc tgctccatta
    56401 tatccaaata taaataaaac atctgcattt tctatctctg gtatagaatt agacattgca
    56461 ccactaccta atgagtaagc cagaccggct acagatggtg catgtcagac acgagcacaa
    56521 tggtcaacat tattagttcc tattgtagct ctcataaatt tttgcattat atagtttgct
    56581 tcattaccag gtcctcttgc agaacctgtt cccataatag aatcaggtcc atatttttct
    56641 ttaatttcat ttagtcttga agctgtatag tttatagcct catcccattc aacctcttct
    56701 agtacacctt gttttcttat cattggcttt tttagcctag gtgttagaat tttagggtca
    56761 tttagaaaat cccatccata acgacctttt aaacacaaac tttcttgatt tgttctacca
    56821 ttagccggtt cagctcttaa tatcttatta ttttttacta ctagatataa ttggcatcca
    56881 gctccacagt atggacaaac tgttaaaatt tttttctcca tacaattccc cccttagatt
    56941 tatcccctga taagtaaaat ttatcttaaa gataatgagt gtttttataa tatgcaaaat
    57001 cataaatatt ttgcaatact atctattaaa ttcaacaatt ttctcacaat tcctcttaaa
    57061 ttttaaaaaa aatatgtcat aataatcttt atatttcaac catttaatta aaatattcta
    57121 gttcttatga catatttttt aatatactca atatatattc tttaaaccta tttagttttc
    57181 acttaaaaca atatttctgc caattctatt tttcattcta tagtgagtaa ttacagtaaa
    57241 tattccactt ccaatcagaa tcaaatagta cataaatcca ccatataaaa atactgcata
    57301 tcccatcaaa ggttgaggga atactgattt gaatatagtg aaaaatgcta attcattagc
    57361 tcctacattc ccaggtgttg gtattggaga tattgccata tataaaaatg cttgtaatgt
    57421 cagtatataa atatagctat aaccttgtaa attaaatgct ttatatatcc aaaatgaaac
    57481 actaaagtaa gcacttaatt gaataaaagt tactattata gttgaaatta ataccttttt
    57541 attctttgca aaaaaattta ttgctttact ataatcatct ataaaacttt ctatggattc
    57601 aatcttatta tccaaaaatt ttaaaaactt aatttttgat aaatgcttta tagtaattct
    57661 cataaaatat tttatttttt tagggtttaa aataactaat attatcataa ttaagacaaa
    57721 tgaattaatg gctatcccta gaaacactag aggcataatc attttcattt gtttttctaa
    57781 catagcaaaa ttcattaata caagtattgt acaataaaaa gtaactacta tttggaaaat
    57841 aatagactta ttggtaacaa ctgcacttgc cttactaagt ggcattttac attttttaag
    57901 tacatatatc tgtactggtt ggcttcctga agcaaaagga gttattaaat tataataaaa
    57961 tcccataatg gctagcttaa aaccaataaa tctaatatta actttgtgag tgctttctat
    58021 aattatcttc attactactc cttcaagcat tatgtgacac attattgcca aagcacctat
    58081 gaataaaaac ttcttatcta ccataaccat aacatttgat agcattttaa tatccaatgt
    58141 tttaaacact aggtaaattg ttatacctat taacaaaaat aaaaatgtat actgtagtaa
    58201 actctttcta atattcttta gtctatccat tagaaataac catcctttgc tcaagattta
    58261 tactaaatac tatattatta gtatacacta tgaatcttaa tattttataa caaaatacct
    58321 tacaattttg tcatttacaa aaaatattaa tctataaatt aaattttaat atatagatta
    58381 atattttaca aacattttgt aaatatatat aatattttca ctaaacaaca cttatactta
    58441 ttacacccat ttatacccta tcccccatac agtttctatt gtataaatat catattgttt
    58501 taatttgctt cgtatatttt ttatatgctc tgtgatagta cttatatcac tttctccatc
    58561 aaaaccatat accgactcat atattctttc ttttgaaaat acttgtccct tattttttgc
    58621 tagaaattca catattagat attcactttt agtaaaatta atttttttac catcaattaa
    58681 aacttctttg cctaaaaagt taaattgaac atttgagata gttaataaat tctttttttc
    58741 tctactttct ctccttaaat gagcagttat cctagccctt aattctccta caccaaaagg
    58801 tttggttata taatcatctg cacctatacc aaaaccatac ataatatcac tttccatatt
    58861 ttttgctgtc aaaaacagta ttggacaatc aaccttattt cttatagttt tacatacttc
    58921 aaaaccatcc atcttaggca tcataacatc taatattatt agattaaaat aattaaaatc
    58981 catgttcata gccttttcta catcactaat tacctttact tcatgtccat ctcttttcaa
    59041 cacattttca atcaacttta acatatctaa ctcatcatct actactaata tcttagacat
    59101 aataactcct ccatttatcc atctaatagc attctaattt atatatttaa taatatcttt
    59161 aaaattcaca ttaaccataa tactctcata ttatagctta caataattaa ttactatttt
    59221 acaaataaaa tattttagat acaaaaactt tatatttcaa aaactttata ttcatttta
//
LOCUS       DAEMAE010000004        59103 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEMAE010000004 DAEMAE010000000
VERSION     DAEMAE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.004"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(541..2217)
                     /locus_tag="KPH93_000190"
     CDS             complement(541..2217)
                     /locus_tag="KPH93_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1687034.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHQAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(2318..2470)
                     /locus_tag="KPH93_000191"
     CDS             complement(2318..2470)
                     /locus_tag="KPH93_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687035.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(2959..3369)
                     /locus_tag="KPH93_000192"
     CDS             complement(2959..3369)
                     /locus_tag="KPH93_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1687036.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(3893..5212)
                     /locus_tag="KPH93_000193"
     CDS             complement(3893..5212)
                     /locus_tag="KPH93_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1687037.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNRISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(5209..5862)
                     /locus_tag="KPH93_000194"
     CDS             complement(5209..5862)
                     /locus_tag="KPH93_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687038.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(5960..7336)
                     /locus_tag="KPH93_000195"
     CDS             complement(5960..7336)
                     /locus_tag="KPH93_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687039.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFDNITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(7336..7992)
                     /locus_tag="KPH93_000196"
     CDS             complement(7336..7992)
                     /locus_tag="KPH93_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687040.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(8002..9279)
                     /locus_tag="KPH93_000197"
     CDS             complement(8002..9279)
                     /locus_tag="KPH93_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687041.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQEIYTGLSSDFSENIVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(9298..10188)
                     /locus_tag="KPH93_000198"
     CDS             complement(9298..10188)
                     /locus_tag="KPH93_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1687042.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLLQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRSAIGFLYYWKGIILIVTLLLSIIMY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(10181..10276)
                     /locus_tag="KPH93_000199"
     CDS             complement(10181..10276)
                     /locus_tag="KPH93_000199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG1687043.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(10366..11523)
                     /locus_tag="KPH93_000200"
     CDS             complement(10366..11523)
                     /locus_tag="KPH93_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG1687044.1"
                     /translation="MKKIVSRGACLLLALCLSLNAVGCQKSNENNKIKEGQSISSEEA
                     GMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYIADKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSAKEWKQVIEKELEKIKNK"
     gene            11832..12182
                     /gene="mobC"
                     /locus_tag="KPH93_000201"
     CDS             11832..12182
                     /gene="mobC"
                     /locus_tag="KPH93_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG1687045.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATNNINQIAKRVNSTGVIYKDDINDIKNEIEHFSKELW
                     QIHSLLLNRTSGGD"
     gene            12188..13519
                     /locus_tag="KPH93_000202"
     CDS             12188..13519
                     /locus_tag="KPH93_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG1687046.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGEKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPEMAHQIGMELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYHSNKKSYHQIRYQSDKLCKENNLSVIDKFYESYKRK
                     YKTNGKSWYENEQSKKGTSWKSKLQFDIDRLIKQSKDWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKQRFTRAKTIGEDYTEERLKERITENQTIKTPPVKKRIGNVIDMNTNVKVKE
                     SKGYEYWATKHNLNTMAESVIYIREHGIKSVKQLDEYIQKVADERQNLQDKIKIIDKE
                     MQELSTTMEKVHTVKKYKQYYMEYKANPSDKAFFEEHKAEITRYETALAKLKKSYSKL
                     PDSKGILDKLDKLQEKKNTLMQEYSSTKSTMDELYQIRKNYGIYMDKEMER"
     gene            complement(13546..15228)
                     /locus_tag="KPH93_000203"
     CDS             complement(13546..15228)
                     /locus_tag="KPH93_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG1687047.1"
                     /translation="MNVIFDKIYIFDVVKKKAYSTEFKKGVNIITSSDIDGTDRGKSV
                     LLRSLYHALGADSHFDSKWGEKDKVYILFFKVDDKDFSIYRSQKLFKIFNAKGDLIFT
                     TIHRSELADFFGNLFNFSICLPQKNTNQLVIAPPAYSFLLNFLDQDEYIGTKFNSFKN
                     LAQFSNFKLNVIYSHLGIYNKEYFELVKHKEELELEIKSKKEEIEDLIKMKDRTILIL
                     DGFSCPETSSALENELNIETQKYSSLINEMNLVRNKLVDLRNQLVEQNITLSQISKFE
                     IKEEKEIKTILKSKVCPECHSVLDSTLELRSKRYNHIDNALSLKDAIKTENAHIEDEI
                     LKNEGIYSNLTLVLEEHNKKICKNKKEIKDYIKFRGLNKLIDEINNDLTINSRVIDST
                     KEALKPLKKEFKSVKDKIKSVDENYYYLIDKLKIRFNLNELEADSYEQLSKNFCASGS
                     NKPLSTVIWYLTLNDLKQKFNPEGTTFPMVFDSPNNAETDQEKKQALVEYIMESSDQF
                     SQLIVSAIGFSEKDYSIDSNINIKVLENEKYSLLNSEMYVQNYELLQCMNDA"
     gene            complement(15228..16256)
                     /locus_tag="KPH93_000204"
     CDS             complement(15228..16256)
                     /locus_tag="KPH93_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBG1687048.1"
                     /translation="MSKEKYYMNLPKDLSGSIAKNRFRLELLWGISKIIDEHKANNEY
                     TIIFDFKCDIELHKEDELDFYQIKTKKSGNYNSNNLCKKGKNKNNSILGKLYALYSPN
                     YNIKLAIVCNKQLKINNKEIDFPEQCFGDLDQDVLDDVRKKLCTELNLDTVCLDNVFY
                     IFDNMDLLNPEDSIRGKLVKSFVDIKGEEPQNPNALYRLVVDSVKEKASYEFDSGTYE
                     DVVKNKGITRSEFDKMLNAHKKESKNGINETQEYINNLSFAKRRRYNTALGNILETQQ
                     SESLRLIKIKIYNYIVEHENSLDDIESYLKEISKLFDDDFDVEFTDDMKSVQYIMIYY
                     MYASGGIL"
     gene            complement(16277..16489)
                     /locus_tag="KPH93_000205"
     CDS             complement(16277..16489)
                     /locus_tag="KPH93_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687049.1"
                     /translation="MRAFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEEIVECIRIDK"
     gene            complement(16630..17289)
                     /locus_tag="KPH93_000206"
     CDS             complement(16630..17289)
                     /locus_tag="KPH93_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1687050.1"
                     /translation="MDYKTMINQIEDMVNYNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLREQGQIKDLPYVQEEKDNLINIVGNIVGEVDVVEREN
                     KNGEAFKVANFSVVSKDDEGNKVYHNCSAYGGKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHRNIRILSSKLLKEKEQMKGQEEKKESVLGAIKKYQAEDKEKPKEKKEANQEAE
                     R"
     gene            complement(17332..26313)
                     /locus_tag="KPH93_000207"
     CDS             complement(17332..26313)
                     /locus_tag="KPH93_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG1687051.1"
                     /translation="MRINDFHNILELVKQDILQSEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHKDVLKEMIKTEGYEESESTLENIFSLSRLYGDEKIDNLMNELR
                     IADEDRISFTKFVRDSISYAVASRFKLDYPMDKELLKENFAMLDSISLMSLGETVSDI
                     SGTVIDTTIQKSKELELKKEVLRGKEAGYNKIKEELEEVEENVLRRDDQERISENERI
                     FRNGKYRRDNRENQGEYTESVRGRDGLYEGISESSVRSDEAELSFKQRRAEPLRDVSG
                     SIQGEEADQTPDGYSETGDRVYESRKAKTDDVLADREREPSSVQSNDFSPQRNDNQGS
                     SGNLKENTDKEIREADKASFSLPENSSGQIKLTIPLAQNDIDSILVNGGNHEGGRLPI
                     IAEFSKGKTVEKLGEYLKDTMGGGNGFFLNGTEVSSWYSDKGIHLAYGTSAREDMTQI
                     LSWSDAARRINELLENGEFATNVELLEAQDYERNRVSESLWYLYHDLSEEGKAQGYFD
                     FIETGGGFPKETRQLSEALKNPDYLKDVVSEYSRFLTGYKENKEVLRFHYHKVDSLYQ
                     RLQELALTRKEYTSNLIELPKIQGFITEDEVLATISRGSGVDKGKERITKFFKENHTL
                     QEKANFLKGEYGIGGGSHAVSGERGSGEWHDAKGLKLQKNHCNDVFLTWSNVAKHIDE
                     LLSKNLYLEEKNIEKKVEIEEQKEPSYYSKDDPYNLITDEMLEKVPELYAQEDVALAD
                     KQVHAAYIIPFRSNWTWYMTEYDRESKDAFGLVLGIEPEWGYFNLDELKELNAQRLVL
                     EDFPKTFRELKDSELKKQMDEQELYQVFNGELSFEENNIELEAPEDVEERKQAEPFQA
                     TLFDYLKEKEEVELNEKENSLSDEFTVKEGDTVYFNHEEYKVRDLSKNQITGKNDLWL
                     DPVRQGNHQIPIVAFTDNEDLLRQISLERPEFIIGDEVKYKGKDYAITKFDNMGNNLK
                     TVTVKDNTEYLGGMITGSDVIPYRQESELNKIFENLTYKKEELYDLAVRIDEIAYDFD
                     SYDYADSVEAESEKRLDNINQIYGDLQAGNVSVYINSLNEMIEEDVEDTEIIKQVLTQ
                     LEEKKIDIQPKQEVFNFKITEETLPQRLSPSERLNNNLEAISMLNRVESGERELDITA
                     QEVLAKYVGWGGLSDVFDESKDGQWKDARAFLKENLSSSEYEAARESTLTSFYTPKIV
                     IDGIYKTLSGMGFKQGNILEPSMGIGNFIGNVPDEMGKSKFYGVELDSVSGRIGKLLY
                     PESEVQVKGLEETGFSNNFFDVAIGNVPFGEYKVNDREYNRNNFLIHDYFFAKSIDKV
                     RNGGVITFITSSRTMDKKDESVRRYLAARAEFLGAIRLPNDTFKGVAGTEVTSDIIFL
                     KKRDSVLERDEAWIHLSEDKNGLVYNKYFVDHPEQVLGSMREVSGRFGKTLTCEPIAY
                     LGQEINMASLKERIEIAGERISKDTKYEEIELLDDEITSIPATDDVKNFSYTLNDDEV
                     YYRENSLFIKKEVSDKNKEKIKDYLELNVALKDVIYKQKEDFSEEEIKASQEKLNEVY
                     DNFSKKHGFVNNLSNTRALKEDSNFPLVSSIEILDEEENFKAKGDIFSKRTITKAKVI
                     DHVDTSLEALVLSVSEKGYVDFDYMGSLTGKDRATLIEELRGEIYLNIREEQNFYRPL
                     SFNPEDGDLPFACANGSNSYKYGYVTKDEYLSGNIRDKIAIVDSYLSKLRQTERELPH
                     LGFVENGKEKELISYEMNRLEYQKAELTKVLPKELEASEINVRLGATWIPIKDIEKFI
                     FETLKTPGYARWDIKVKFSNITSEWNVEGKSRDKGNDLAEMTFGTSRVNAYKLIEDAL
                     NLKETKVFDQIINPDGSKTSVLNKKETMLAGQKQELLKEEFKNWIFNDQERRNRLVKL
                     YNERFNSIRNREYDGSNLSFEGMNTEIDLRPHQRNAIARSLYGGNTLLAHVVGSGKTF
                     EMVASAMESKRLGMCSKSLFVVPNHLTGQIGREFMQLYPSANIMVADKKDFEPKNRKR
                     FIGKIATGEYDAVVIGHTQFEKIPMSKEYQEKHIQDQINEIVNYVEEYKHDRNQNFTV
                     KQLEKTKKKLETRLEKLNDDFKKDDIITFEELGVDMLFVDEAHNYKNLYLYTKMRNVA
                     GIGQSEAFKSSDMFMKCRYMDEMTGGKGIVFATGTPVSNSMTELYTMQRYLQYESLKK
                     NHLEHFDSWASTFGETQSSFELSPEGTGYRVKTRFSKFYNLPELMSMFKEVADIQTAD
                     MLNLPTPEANYEVIKTEPSEEQKEILKSLSERADDVRNRVVEPDVDNMLKITNDGKKL
                     ALDQRLINPLLPDNPDSKVNVCVKNVFAIWDKTKENRSTQLLFSDMSTPKGDGEFNIY
                     DDIREKLVAMGIPKEEIAFIHEANSDKQKDELFAKVRKGEVRILMGSTQKMGAGTNVQ
                     NKLIALHDLDVPWRPADLEQRAGRIVRQGNENEKVNIYRYVTENTFDAYLWQTIENKQ
                     KFISQIMTSKTPVRVAEDVDESSLNYAEIKALATGDPKIKEKMDLDNEVTKLKMLEAN
                     YKSNRYRLEDKVAKNYPEEIARTEKLIEAVKKDIASVEPKAEGEEKFTSITIAGEKIN
                     DKKLAGERLLEAISKVKINESKVIGKYRNMDLEVSYNFFTNEHNFSLNGAAKHSGELG
                     TSADGNITRLDNALEKMPEKLNRLEEKLISTKEQLQNAKEELKKPFEKADELKTKVLR
                     LAELNKLLDMGEVEEKRNDNPLVEDVKMAIIDFCNREYEENHSYDEFNTLYPDLKHIG
                     IAYTNTPDERHGIQYELDLEEKTWTQYIDGTPIKTESFYYENKGENEALRNMKNEIEL
                     FSFNDLVYVDSEDLKAALGLDIDDEGNFYDPLSKDLDNDGIADRYDNDFRDSDYFEST
                     YDVEDNLNTKVESVTKAEDKPSILSQIRAYQNESKTEEKQKTKEQEILR"
     gene            complement(26426..27232)
                     /locus_tag="KPH93_000208"
     CDS             complement(26426..27232)
                     /locus_tag="KPH93_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1687052.1"
                     /translation="MRTGNEFNGVEDTLYIPLAVRIYVSEKFPHFFYDEKALSLKPKI
                     PTGGIDNNSAEYFYMASVCRQQTIDMKIKKFLDENKKGNVVFLGAGLETSYNRIGNQI
                     VNFYQVDLPGVIEVRKRVLGNAKNEKLISGDMFDLNWIKEIDTSLPTLISVAGVYQYF
                     DEYKIIDMIQKLKAQFSKGELIFDATNSKGLKLANKYVKKTGNKNAQMYFSVDNSKDL
                     ADKTGTILLEVDGFFHGALKACQGLKLMTRLYMYFADKLHRTLVIHLRFN"
     gene            complement(27343..28389)
                     /locus_tag="KPH93_000209"
     CDS             complement(27343..28389)
                     /locus_tag="KPH93_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBG1687053.1"
                     /translation="MNKEKNSLQNINIEKNNNSFEEIVMIVENAKDRAYRKVNEELIL
                     MYQEVGKYISEKTKEASYGSGFVDNVAEFFSINYPDLKGFNRRGLYRMKQFYELYKDD
                     EKVSTLLTQLSWSNHLKIMSGAKSREEREFYINLAIKENLTHRELVRQMDSGYYERCM
                     LSNEGNMPAIQRAKQETHNLFMDSYVLEFLDAPKIGNERDFQKSILENLKNFVLEIGK
                     DFSFIGNEYRVQVGNHDYYIDLLFYHRGLSCLVAFELKIGEFKPEYVGKMNLYLEALD
                     REVKKQAENPSVGVILCASKDDEVVEFALSRSLSPTMVSEYRLKLIDKELLQRKLKEY
                     TGIAEQEIERHDEK"
     gene            complement(28423..28608)
                     /locus_tag="KPH93_000210"
     CDS             complement(28423..28608)
                     /locus_tag="KPH93_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG1687054.1"
                     /translation="MSEVFMNNAKVMAKGQVTIPKKIREILEIENGDYVTFVVTEGKI
                     QIVNSKTFIEKNIQGRK"
     gene            complement(28692..30398)
                     /locus_tag="KPH93_000211"
     CDS             complement(28692..30398)
                     /locus_tag="KPH93_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG1687055.1"
                     /translation="MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLI
                     QMANPESYDEKYAKWNMADLPIIPREYKYEIAKSTKKQFTILKKLMNDKEIDIVINAC
                     DAGREGEAIFRLVYNQANCKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQA
                     RAIADWLVGMNLSRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELD
                     LDGFSLSTDRIDDKTVTEQLINLLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKY
                     LNYSAKQTLDYTQSLYEKKLITYPRTDSRYLTEDMITSTINNIIGKHDFDTGRIKMVF
                     NSKRVTDHHAIIPTASSMNEDLASLPESELKVYELVLNKLHASVGYPLIENTTKIVAV
                     FDGFEFTSSGKVIVDEGFTKYLKKYIKKKNEDTVFPDLKIGDSLDIKEKEIKEKYTSP
                     AKHFTEDTLLKDMELAGNDALEKGVEVERKGLGTPATRAGIIENLIFKGFVERDKKNL
                     IATHKGISLVTIVSDTFKSAGTTAKWEMELSDIAQGKASKENFLKAIENEIKELVSTY
                     SK"
     gene            complement(30494..32068)
                     /locus_tag="KPH93_000212"
     CDS             complement(30494..32068)
                     /locus_tag="KPH93_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG1687056.1"
                     /translation="MKKMKNKKAAGVILGAVLLIVGVVISLFAGSKIVFAQGGLFTTL
                     VNPIEAVAQIEVEVNVKYIFEDEKVFKEEKIKAEKGWLLDSGDLPIIPDDMKFIDEFL
                     FYEVKGDGKDEIIRKVTKIDVKDKETQTENESSKSEQSSQSNASKIENKGTQTELSKD
                     DISKMERESKELKEKLDKLNQEIKDKDKLSEKQKDKIKDLEEKIDKLKEKLEKGKDDK
                     DLSADMKKEIDKLTDKIKELEKKANEVGRAPIMHNPNVTPISPISGIKQGTGNVSPQT
                     SINSSGKGSATQESKGANTKDTNSNESKEKEKQVRYPNKLTPKQPANNGSHDSSLDGS
                     SKNINTNKGVASEPSKARGTVTENKDNANKNYPIHHNDSKDNKETDKYSADARQFVTF
                     TTKNGKTFHLIINHDEDSENVMLLTEVSEDDLLNMVEKKAPEKEIVKEESQKEEPKQV
                     KKEESSNTGTYLILILVVAGALGAGYYFKVIKKKEDKELEGFEEEDDDFFSEAEESEN
                     EVNEAVTEDKEDDEIE"
     gene            complement(32049..32282)
                     /locus_tag="KPH93_000213"
     CDS             complement(32049..32282)
                     /locus_tag="KPH93_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687057.1"
                     /translation="MKKELTAVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLMAKVNREKSEMKKENGNEKNEE"
     gene            complement(32294..34456)
                     /locus_tag="KPH93_000214"
     CDS             complement(32294..34456)
                     /locus_tag="KPH93_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1687058.1"
                     /translation="MGKKLKKDFKERQRARIEKETLQSNMGIEPEESKLKYNDDYRGK
                     IVHDKDRFQDKVHEKTSKMTSDSEPVRGTSKRNARYRASNKDSITSSTETGGVSQEEI
                     GKADYGTEVKDGKIYDPLGKDLDNDGIIDRYDNDFRDSDYFESTYDVDGLKKDEISGN
                     PVNKQNTQKKNYKRKNYSDKLYTRKKDKEAKEEKADNKKSGKEAVLDREKKNRLSKEQ
                     ETIFENKTSKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKNY
                     SDKKRTKKGYDLTNKDYKIRKRKSKLEFRDAKEELKKTEEYQKANNFKRFQKRKQMKD
                     SIKRQNKSRLRDRIKEGLIGSLKSSKEVILRKTKGLMIIFIGLILLGTFFINFAGTGM
                     TGFMNSTSTVLTTSYLSKPNVLTEIHQQFSSMEQGLQDELESVEENYPGYDEYIVNKE
                     GDIGHNTHELLSYITSRFGEVKNVSEVSSVLDELFKSMYRLEYKEEIEIRYRTVTEIY
                     TDEDGNEYTESHEEPYEYKKLIVTLYKREMDSVVREVFQNYPDNVKHYEALLASLGNM
                     SDFFGSGSNDYSEIINNPNFGNPGIAFNEETVKRLFSEAERHIGKRYVFGANGPSNFD
                     CSSFVCWSFTHSGIKNMPRTTAWRIYTDYCNPVSTGEAKAGDIIFFKNTYNSGSPISH
                     VGIYAGNGMMIHAGDPIQYTSINSPYWREHFYGFGRPR"
     gene            complement(34461..36890)
                     /locus_tag="KPH93_000215"
     CDS             complement(34461..36890)
                     /locus_tag="KPH93_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687059.1"
                     /translation="MNKKRKIQQLKLEQNKKKLRSQRQDLKQNKGKSKKDKGGLLDPI
                     FRKEPKRYTVEDTIPYLRLLKSGICQIDENHFNKSIAFEDINYQLALEEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKGDGFDDIRLEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERMEIDILSSFKSMGVRAESLNGEERLKILHD
                     VLNPNKTFEFSYKDLKKKESTKTYIAPDEFNFTPSRYFKFGNFIGAASHFQILASELS
                     DRMLSEFLDIDDNINISFHIKAIEQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVTIIFMNFARTIQKLDNTISQISSIANKH
                     NCKLKRLDHSQEQGLISVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSANSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILTTDDDVIICDPEG
                     EYGNLVRQFKGKVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYGKYFENPILENMPILQDLYDMLKNQEEKVGKKLATEMEIY
                     VTGSLNVFNNRSNVDLNKQLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSNEIENIFDNTDF
                     VLMLNQASGDREILARKLKISQPQLKYVTNSNAGEGLLFFGNTIVPFLDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(36802..37191)
                     /locus_tag="KPH93_000216"
     CDS             complement(36802..37191)
                     /locus_tag="KPH93_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG1687060.1"
                     /translation="MAYVPIPKDLKKLKTKVAFNLTKRQLIGFTIAGLIGIPIYLFMR
                     KVVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QKKKDSAAKVRTKQKEVKKSKTGLKAK"
     gene            complement(37195..37455)
                     /locus_tag="KPH93_000217"
     CDS             complement(37195..37455)
                     /locus_tag="KPH93_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687061.1"
                     /translation="MNKRKTVFIVVIGAGIMAVIDRIRLHSKVNDLEERKKDIGRCHN
                     DFCLMQERFNRSIDEQITSIQEEIGSVYEHIEELSKGKEDGR"
     gene            complement(37473..38336)
                     /locus_tag="KPH93_000218"
     CDS             complement(37473..38336)
                     /locus_tag="KPH93_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687062.1"
                     /translation="MFGIFDKIEEFFKEFLLGGIQANLESMFLDINDKVGAIATDVGK
                     TPMGWNGQVFSFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFDFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSATVSGNQIVAMIDSLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYIYSSVSAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIKITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(38356..38571)
                     /locus_tag="KPH93_000219"
     CDS             complement(38356..38571)
                     /locus_tag="KPH93_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG1687063.1"
                     /translation="MEFFTHAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLR"
     gene            complement(38575..38886)
                     /locus_tag="KPH93_000220"
     CDS             complement(38575..38886)
                     /locus_tag="KPH93_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-strand DNA-binding protein"
                     /protein_id="HBG1687064.1"
                     /translation="MKQEMININANLLAEPTFSKFEKEGEKVEVANFTLVKKYGKGKE
                     YINCAAYGEKAKTVKAFEKGDLIHIFGYFKKREKDGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(39095..39962)
                     /locus_tag="KPH93_000221"
                     /pseudo
     CDS             complement(39095..39962)
                     /locus_tag="KPH93_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(40126..41907)
                     /locus_tag="KPH93_000222"
     CDS             complement(40126..41907)
                     /locus_tag="KPH93_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG1687065.1"
                     /translation="MIDKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNTMSFLPSFHPTDILIGVGVATLIKFIAYTKGKNAKKFRQG
                     KEYGSARWGTRKDIEPYIDEKFQNNILLTQTERLTMNGRPKNPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDDETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIRELRDLMGEDELELDTLGDRKTALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNQK
                     SFGLNYQKTGKELMSQDEITVMDGGKCIFQLRGVRPFLSDKYDITKHKNYKLLEDYDK
                     RNLFDIESYIKRKGKVKMNRHTVITRL"
     gene            complement(41904..42389)
                     /locus_tag="KPH93_000223"
     CDS             complement(41904..42389)
                     /locus_tag="KPH93_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG1687066.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKGLLNLLKKLMKEAEKFGGLEK
                     LVNVNGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFEEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(42391..43401)
                     /locus_tag="KPH93_000224"
     CDS             complement(42391..43401)
                     /locus_tag="KPH93_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687067.1"
                     /translation="MYLFDEQPIVANKTLARELGLNEALVLQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYKNIYQ
                     NCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKISVQVV
                     KERFRKLNSGHLEYLVGVLRENEYKIRNIRAFVLTAAYNAPSGIDAYYSALVSYDMRG
                     GY"
     gene            complement(43502..44020)
                     /locus_tag="KPH93_000225"
     CDS             complement(43502..44020)
                     /locus_tag="KPH93_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBG1687068.1"
                     /translation="MRVYENKEELKTEINKTFEKYIAEFDNIPESLKDKRIDEVDRTP
                     AENLAYQVGWTTLVLKWEEDERNGLQVKTPSDEFKWNQLGELYQWFTDTYAYLSLQEL
                     KSKLNENINSIYVMIDSLSDEELFKPHMRKWADDATKTAVWEVYKFIHVNTVAPFKTF
                     RNKIRKWKKIVL"
     gene            complement(44061..44342)
                     /locus_tag="KPH93_000226"
     CDS             complement(44061..44342)
                     /locus_tag="KPH93_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687069.1"
                     /translation="MRWILKIILFPISLLLSILTAVLSFLLGIGTVILYLLMMFCIFG
                     AIASFLQKEVTIGIEALIIGFLLSPYGIPMVGASIIALLQGINEKIMSV"
     gene            complement(44361..45995)
                     /gene="rlmD"
                     /locus_tag="KPH93_000227"
     CDS             complement(44361..45995)
                     /gene="rlmD"
                     /locus_tag="KPH93_000227"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG1687070.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKNTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     MREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(46170..47930)
                     /gene="pyk"
                     /locus_tag="KPH93_000228"
     CDS             complement(46170..47930)
                     /gene="pyk"
                     /locus_tag="KPH93_000228"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG1687071.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIICVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGDACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(47982..48941)
                     /gene="pfkA"
                     /locus_tag="KPH93_000229"
     CDS             complement(47982..48941)
                     /gene="pfkA"
                     /locus_tag="KPH93_000229"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG1687072.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(49150..52719)
                     /locus_tag="KPH93_000230"
     CDS             complement(49150..52719)
                     /locus_tag="KPH93_000230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG1687073.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     IDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRDIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEISKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGVLTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(52870..53823)
                     /gene="whiA"
                     /locus_tag="KPH93_000231"
     CDS             complement(52870..53823)
                     /gene="whiA"
                     /locus_tag="KPH93_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG1687074.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEILLK
                     KLGILENKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKSSQ"
     gene            complement(53840..54265)
                     /locus_tag="KPH93_000232"
     CDS             complement(53840..54265)
                     /locus_tag="KPH93_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1687075.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(54334..55440)
                     /locus_tag="KPH93_000233"
     CDS             complement(54334..55440)
                     /locus_tag="KPH93_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG1687076.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(55452..56309)
                     /gene="rapZ"
                     /locus_tag="KPH93_000234"
     CDS             complement(55452..56309)
                     /gene="rapZ"
                     /locus_tag="KPH93_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG1687077.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(56340..57053)
                     /locus_tag="KPH93_000235"
     CDS             complement(56340..57053)
                     /locus_tag="KPH93_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG1687078.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMEDKFIRYFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYILKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(57057..57971)
                     /gene="murB"
                     /locus_tag="KPH93_000236"
     CDS             complement(57057..57971)
                     /gene="murB"
                     /locus_tag="KPH93_000236"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG1687079.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(58244..59014)
                     /locus_tag="KPH93_000237"
     CDS             complement(58244..59014)
                     /locus_tag="KPH93_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG1687080.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        17248 a        11756 c         7169 g        22930 t
ORIGIN      
        1 tatgtagttt taattataaa aaaagatgaa caaaaattat acagtttaat atggaatcag
       61 taggtacata tgtattatta agaattataa ataataaaaa caaattttag tcaatataca
      121 aaaacctttt aaattgtctg gaaatcacct cttcatttgt tataaaaaca tacaacaaat
      181 gaagaggtga ttttatggtt tttaaaaaaa taattataag tggatatgtg ctttcacatg
      241 tttatcaaca tctgtaatgc ctatatttac tagtgaatta taatataaaa atattaaata
      301 atattcaact atagattatg taaattattc tatggaaaaa atgagttgta taaaatctgg
      361 caactgtaga ttgacgattt cttttaaaat tacaattata ataagttcta tagaattaaa
      421 aataaaactt tagagatttt tgataacatt gtagactaaa tttgccacca aaattacttt
      481 acagtggcaa atcggtggca aatacaatat attattagta tataatctat ataaacatta
      541 ctattgtttg attatcttag ctacagtttc aacttgctca tcattttcca aacttatatt
      601 aagatcttca tcaatgatag gtagcttaaa agtaatggat tttagccatt gtccattagg
      661 ctgcttttct tcataaattt gaatttctga aatgagtgct tctattaact gcctgcgttc
      721 aacatcgttc atgactttat aaagcttgtc aaaataaatc aatactttat atatgttatc
      781 accagtaagt ttctcaacct ctatcgtttc tttttttgct ttagcttcta ttaactgact
      841 ttctaattcc tcaattttat cgtacatacg ataaagccta tcatctaaat cattttttct
      901 tctcttataa tgtttatcct ctacatctaa attatctatt tcctcaataa gcttatactt
      961 tgtagaatga ctttttctta gctcattttg gtaattatct atctcatttt caatagcaga
     1021 tgtatcaacc ttcatgttaa ttttttcttg tatcatagat gcaaatttag ggttgcttac
     1081 taacttgatt attacctcag caacggcatc atctaacaat tcttctctaa tttgtttatt
     1141 ataggtacat ttatgtcctc tattcataag tctatgctta caaccatagt aatagaagtc
     1201 tttatacttc gtcccatctt ttttatattt tatagattta ttcccaaaca ttccagctcc
     1261 acatatcgga cactttacta ttcctgataa taagtgtgtc ctcatgttct tgcccttatt
     1321 tacatgttca tactttttag cttgtgcttt cagtttaact tgtgcagcct gccacaattc
     1381 atctgggatt attgcttgat gaatgccttc tgctactaaa taatcgtcct gctctacttg
     1441 tctgtattca ttccttgtac catgaacttt ttctaaagtt cttcttccaa atgctatttt
     1501 cccgttatac acaggattct ttaatatttt tcttataaga cctgcgtcaa acaatggatt
     1561 tttcccattt tgtcttggta ttttccttat accatgattt tctaaatatt tagaaagacc
     1621 attagctcca atacttgtat ttacatattg atcaaaaata gttcttattg caattgcttc
     1681 ttcctcattt attatcaatt tcccatcaat aagttgataa ccatacggag caaatccacc
     1741 attccattta ccctcttttg ccttttgaat gcgaccttcc attgtttgga cacgaatgtt
     1801 ttccctttct atttcagcca ctgccgataa aacagaaatc atcaatttac ccgcatcttt
     1861 agatgaatca attccatctt caacgcaaat aagatttact ccaaaatcct gcattatttg
     1921 taaagtcgaa agtacatcag cagcatttct tgcaaatctc gatagtttaa atacaagaac
     1981 gaaagacact ccatcctttc cggattttat atcttccatc atacgagtaa actgaactct
     2041 accttctata gattttccag acttacctgc atcttcgtat tcaccaacaa tctcataatc
     2101 attatagaga gcaaaagcct tcattcttga tttttgtgcc tctaaagaat aaccatctat
     2161 ctgtatggat gtagatactc ttgtatagag atatactttt attttttctt ttgacatagc
     2221 attaacctca atatccttat tatatattta tatattttta ccaataaaag tttggacttt
     2281 aatctcaagt atattatagc acttttattt ctctttctca atccgtgctt ttagctatat
     2341 caaatttatt cttatcctct gtatttttta ctggagtagg atttggaaga ttctctaaat
     2401 ctaaaactcc agcatatttt gtaatcagat ttgctatcaa atcagccaat ccattccagt
     2461 cattatccac taatacacct cctccacttt aaattcttta taaccaaata ttatttttct
     2521 tactaagttt tatgacattg gtaggtgcat tggctgctac ataggaatct cacctccgcc
     2581 ccttgtttca gacgaggcag cttaaactat tgaagtatca ttatcaccac ttacatcatc
     2641 gaaataagct gccacgctta tcttttatgt attcctatct atcgctcatt ttcttctaac
     2701 ctcggcatta tgccggtatt catttgaacc gattttcatc agcagaaaag tcttggcgta
     2761 ggtcataact ctcagtaagt agataaagtc ctaccttggt ctattcagtt gtcaaagagc
     2821 aatatttgaa agggactgtt ttctttacat tttcctattt gccataagca ggcgtatctc
     2881 ctactcaaga aagtaaagga gatcaaactc cccttcactt tacataagga aaatttgtcc
     2941 cctttggtta ccgaagtttt aaagttcttc gataaatttt ttcagctttt caagaatttt
     3001 gtttcttctt ttgattaaag ccggatgtgt aatacttttg agctttgcta ctgaacgaac
     3061 agtttcatca ttgaaataca agcgttcaat aatatctctt tcttctgcat tgagccttgt
     3121 tatagcactt cttactgctt caatcatcat ctgtgtttca acaatctttt ctacatcaac
     3181 actttcatca acaatattat ctgtaaagtg tccgtcatga tccaatgctg aaaaaaagag
     3241 caagtggttt ttcctgtcca cctgctctaa atacttctcg tgttcttttt ctcgccagta
     3301 gactatatat atatcttcac tgacttttac cttttgtcct ctgatataaa gataatactc
     3361 ttttgccata aagtttcctc catctctttt ttatctgctt gtttttcaga taaaaaagga
     3421 ggacttctgc atcttggcat aagaagtcct ctgaaatgaa agaataaaaa tatttcttta
     3481 tatgaacttg attgtttcaa ttcgcacctg aaacaatcat ctactataca tattataaaa
     3541 aagaggaata tattgaattc taaatatttc taacattaaa cgtttagatg cttccatatt
     3601 tatttcctcc attttatcaa taatctaaat aaccatcata ttcatctact acttatataa
     3661 ttgagtttta ttaaactttt taagtttcta ttctatcaaa tatttaaaca atctctgcat
     3721 ccgaaaagac taactcatat agttttacta acaatatttt tattagcgtg ttcaaatcct
     3781 gatttcttgt atcaattatt ttctgttcac tttcaataag aaaaaagcct ttacttccat
     3841 ggctaataat aagtctctat ttttcaataa ctattgcaaa attatatctt gtttaagaaa
     3901 atattttaat tttaaagata aaaaattctt catcctgcaa tgctttatat tctatattgt
     3961 aattattcag aatattactt acaatatata aacctaatcc attactattt tccttattta
     4021 aatcaaattt aacatcaaat attttatcca tattcgaaat tctattgtta ccgtatgaat
     4081 tctctatata taaccaatca ttaactatcc caatattaat taccccattt acatcagtat
     4141 attttaccgc attactaatc aaattagata gaataatctt taaagctgtt tttcctatat
     4201 aaacattctc atctaatata taattattga cagttatttt cttttgattt gctaatattt
     4261 catacttttt taatatgtct tctaatgtat catttatttt caaatattct tcatcattat
     4321 tcaaattttc tatagaatga actgataata tttgagaaat attttttgtt aaactatcaa
     4381 cgatttcaat acattcatta atataaatat ctctttcttt atattttcca atattgtatt
     4441 tcatgttttc cagtattatt ttaagactag caagaggagt tttaagttca tgggatgcac
     4501 ctttaaaaaa gtcatatttt aacttttcta attttaaaat ttctttattt ttaaattcta
     4561 aatcgtcaat tgttcttaat aaagtagaat acaaatcatt aatttgttgt tttaattctc
     4621 caacttcatc attagagcta acttttaaac gcgttttttt atcaagtttc atcattttat
     4681 cagtaacaat ttttatttct tgtatattat tttttattga ttttgcataa attaaagaaa
     4741 taatagcaga aaataaaatt gatattaata aagaatatgg taaaaattta aggcttaaat
     4801 ctttcgcatc tttttgcata tcagctgtag aaacaaattg tagatttgtt tttttaccat
     4861 cattaagctt tatttctctt tcttcaatta tcaaagaatt actatcactt tctaaattaa
     4921 catttatatt atctttgatt tgtatttcat tcttattatt tttttctttt ataaatgctt
     4981 ttatttcact actcttagaa taaagctcca aagtttgttc tatatatttt atttcttttc
     5041 cattcatatt actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca
     5101 aatatgtttt aggaaagata aaaaaaacta atgaatgaac taatataatt attattccaa
     5161 gaacagaaaa tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc
     5221 caatttatat cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag
     5281 ttcttttata tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac
     5341 ataatcaata atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa
     5401 tacttctagt tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct
     5461 attaaaatta accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt
     5521 aattaaagaa tctattctag cctttaagac cggcaatgaa aatggctttt ctacatatcc
     5581 gtctactaaa ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat
     5641 tagaattggt aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa
     5701 aggtatctga atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc
     5761 tcttccatct ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc
     5821 ctctcttatc atattatcat cttcaacagt taatattttc atatatacct ccacttaagc
     5881 ctatacactt atattataca gcatattatt tgatatttaa aagccaaaaa atttttggtg
     5941 ctaaaaagca ccgaaaattt taattattgt caattaataa ttcttttgga ttccttctta
     6001 atatattgaa tgaagaaata atcaatgata caagaagtac taaactcata aatataacta
     6061 cataaattat gaatttaggt aatacattta tatctaaact tgataatgtc ttgttaaatc
     6121 cttcagcttc tgccccacca cctaattggc tagatgcaga ttgtctagct atttgtttag
     6181 ctatatcacc agtaacctta ttcaatatat tattcccaag cttatcagct gtatattgag
     6241 ctaagaaata agaaccaacg aatgcgggga ttgatataaa taccatctca attaggaatt
     6301 gaccaaaaat ttctaatttt gatataccta atgatagtaa tactgctatt tctttctttc
     6361 tagcattcat ccataagaat aataataatg aaactacaac tcctgcaaat attaatgagc
     6421 caacaaataa tttatttgaa attgagtaga tacctgatat agattgttgt aatgcagggt
     6481 aatttgatga acttttaatc aaattatatt ctctccaatt tatatctaat ttcccaagat
     6541 cttttattac actatcaaga ttcttatcac cttttacaaa gaatgttgca tcttggtata
     6601 cagctgtatc ttctgtatta ccataaactt tagcagctgt atgaacatca gtgattaatg
     6661 tattttcgta tagttcttgt gctgcactta ctccaccgct attatgccca tcgaatagac
     6721 ctttaatttc tacttctact gtttcatctg cacctttctc attatcagca tcaaataaat
     6781 tagattttat ttttatctta tctccaactt taagattatt tttttcagct aaatctttat
     6841 gcattaagat tttattttta tcttcatttt ctaaatgctt tccttctact aatttatatg
     6901 cttctgatac aaattttgtt tcttttgatg agtcgttaac cccagttaac ataactgttc
     6961 ttttaaaatt cttcgctctt tcaggtgatt gattcgcaag agtctcttga gtttcaataa
     7021 tatcataatc aactaaatct gcaacactgt ttattctttt tacataggag tctatactat
     7081 ctgtttgaga tatcttttta atatcttcac cttttacatt tcccccacct ctaggtgttc
     7141 ctggatttac ttgtctattt atctccatag aaaaactatt tgttatatta tcaaatgttt
     7201 cttttgaagc tctgtccgta gcatctttaa tagataaact aatgagactt aaagttgaca
     7261 tagataaaat aactaatata ataattaatg attttaagct ttttctagtt acataagcaa
     7321 atgcattttt tatcattatt caacctccaa attcatttta tttacttttt tcaacttttt
     7381 accacttagt tctaaaatga tatccgcaga atctgctact tccttactat gtgttacaac
     7441 tattacacat ttatttctat ctttagctaa tgtctttaat atattaatta tttctccagc
     7501 agtaacactg tctagattac cagtaggctc atcagctagt attattggag catctgatac
     7561 caatgctcta gcaatagcta ccctttgttg ctgtccacca gataatttca taacatttct
     7621 ttttatttgt tttttatcta aacctagttc aaacaagata ctttcatctg ctgatttatt
     7681 tactaatcta atattttcaa ttggtgataa ataatctatt aaattatagt tttgaaatac
     7741 taaagatata ttattttttc tatgattact atatcctttc ttttgtatat cttcattctt
     7801 aaacaatatt tttcctgttt gaggtttatc aagtccagca agtaaggaaa gaagtgtaga
     7861 ttttcctgtt cctgactttc ctactatcgc ataaaacttc ccaagttcaa atttttgatt
     7921 tactcctgac aaaacttttt ctttagaatt tgcataacta tatgctacat tctttatttc
     7981 taatatatcc attattttct cctaacttat ttttgataat atttctttag gcttcttgat
     8041 taatattaat gaagaagcaa atacaactga taaaacaata atacttatta atattaaata
     8101 actttgtcca agtgttgtta tatttaacat aaaactacta ttatttatta aacttccacc
     8161 ggaaatcatt gagtcctctg agttaatgaa tccatctaca attactttta gtaatacatt
     8221 tcctaaaaat aaggaagata ttatacttgg tatcgatatg aatattaact cgaatataaa
     8281 ttgcattata atttgtatct tagatgttcc aatagataaa aatataccta tttcataaat
     8341 tctttctctt aaccatagaa tcaagattaa tgaaagaaca accatcccac ctaacataat
     8401 agaataagtc attattttta ttatatgttt tattccactc actgactcta aagactcttc
     8461 aaacgcatta gaatcttttt caacaaaata ctttgactca tcaattttaa gctctttcaa
     8521 tttgtttaag gctaagtctg tagattctgc actaccagaa tacattaaaa ttttatttgc
     8581 gattttatta ttttctgatt tatttaatat ctcttggctt gttgaataat ctacaaacac
     8641 gatattttca ctaaaatcag aagataatcc tgtatatatt tcctgttttt taccagaaaa
     8701 gattcctata attttaaatt tatgactttt tatttttcca cttttttcaa tatctagtaa
     8761 ttcaagatca acttcatcac ctaattttag attgttttgt ttagcaaatt cttcatgaac
     8821 aataattgaa ttcttatcat tttctcctat atttttacct tctttaattg taaatactcc
     8881 actactaaat aaaatatttc ttttagtatt atttgtagct tcaagtgaaa caacattctt
     8941 aaattcgtca gataaatctt ctctatttat tctttgttct ccgctaacta ctttagcatc
     9001 ttttagtttt gctaatccat catattgaat tattttttct tcaatttctt ttaatttttc
     9061 aatatcttta aattgattaa cattaaaata tttaccatct ttttttgtta ttgatattga
     9121 agaattagaa ctttcatata aagccttttc tatttcattg cttgatttca ttattgttaa
     9181 acaagaatac aagcaagaaa gaactatagt taatatgata aaaattatta aagttctatt
     9241 tctctttctt gtaatatatg ctatagcatt ttttatcact ttcctaacta ctcctctcta
     9301 ttttcttact ccataactaa ttgcatccac tggacaagca tttttacatc gtccacatct
     9361 gatacactcc agatgattgc aattctcaac tggatctata ttcatttggc aaactttttt
     9421 acattttcca caattaatac acttatcctt atccaatcta tatctaaata ttgaaatagg
     9481 gttaaaaatt gagtaaatag ctccaagtgg acatatatat ttacaaaaag gtctatacat
     9541 aattatagaa agtaacaaag taactattag aatgattcct ttccaataat acaagaaacc
     9601 tatcgcactt ctcatacttt tatttaataa aacaagtggt aatcctcctt ccagcatacc
     9661 aatcggacaa ataagtttac aaaaatacgg agaaccctga cctaaaatat caaccaaaaa
     9721 taaaggtaat attattacaa atacaagtaa tattatgtat ttcaattttc tcaaatgttt
     9781 atcaaactta aatgtttcaa cttttttaaa aaagggaatc ttgtgtagca aatcttgaat
     9841 caatccaaat ggacacagcc atccacaaac aaatcttccc accaatgctc ctataaaaaa
     9901 caaaatccca actatataat aactaatctt atattttgaa tttccaatta ctgcctgtag
     9961 tgatcctatt ggacaagaac cttttgctcc cgggcatgaa taacaattca tacctggaac
    10021 acaaatcttc tttaaattcc caccgtagat tttaccagtc ttaaaccctt caaagtaaga
    10081 attagtaatt aaaaaccaaa cggattgaag taaatgccta aaacgatctc ttattttata
    10141 tttatctttt tttgtactac catttataat atttttaatt ctatccaatt ccaatacact
    10201 ccatacaaat tcttatcgcc ttatccaaga caactttagt ttctccatta ctatgaccat
    10261 agtaaatcat cgccatacta ataaagataa ttaataaaga caaggcacgg ctttttaaaa
    10321 acttattaac catataccaa tcaccgcttt acttttattt attttttatt tattttttat
    10381 tttttccaat tctttttcaa ttacctgctt ccattccttt gcactctttg ccccagaata
    10441 agtatcacct acaatatttc cattactatc aacaaagaaa gtctctggca tagcctgtat
    10501 accattcaat ctaccattaa agtttgtctt atctggcatt aagaaaggat atgaagcctt
    10561 tgtcttttca tgtattaact ttgacttttc aattgcttcc ttgttttcac cgttatcatc
    10621 aacagtatct gtaacaactc caacgatatt tacacccttg cttttcattt cattctgaac
    10681 ctcaaccaaa tcaggaattt cctttacaca agctgtacac caagttgcaa atacatttac
    10741 catagtaaga tcatacttct caaaatcttt acttgtaaaa tccttgccat ttatgtcctt
    10801 tgttgatagt ttacgtaaat ctttaaccga ttctttgtta aatgcttctg tattttctaa
    10861 atcagttttt tctgatagca caaaaccatt tttaggacgt tcttttttat ctataatttg
    10921 aatctcagtt ctttttaatt catccagaag attactttca gctccgctat ttgtgcttaa
    10981 atagcaatcg tattttccat cacttgaaac tcctatttta gtatgagtat cacattttgt
    11041 aattttagat attttttctt ctgatgtgtt tttttcgaat ataccaatag tcccaattct
    11101 ttcaagctca ttttgccaat ttttatatcc atcgcccatc ttttctataa ctgcattctt
    11161 ttgttcttct gtcatttttt caaatgtcaa aatagcatac tttagttctt tatctatagg
    11221 actttgatca tctaacatcg ctatcttttt atcagctata taatttctaa atttgtctga
    11281 gagtttaaat ttaagcccta aatattcata aacatattct ttctttgcct ccaatgtata
    11341 atctgatggc ttaaaagttt catttttgcc atttgcatta attgacatgc ctgcctcttc
    11401 agaacttatg gattgtcctt ctttaatttt attgttttca ttactttttt gacacccaac
    11461 tgcgtttaag ctcaaacata atgcaagtaa taggcatgct cctcttgata ctattttttt
    11521 cataaattat tcctccttta ttttttacac aatagcattt tatagctact ttcaataaca
    11581 ttctacacac cttttatgaa acgttgatga aatataattt tttatttttc caaactatgg
    11641 tataataata taagtattat gcttaaagtt gttcttacac gattagtata tgagcaagca
    11701 cagtaagtgt acggacactt acgaaaaaat tgatgaagca gccctctggg atatgcttct
    11761 ttttttatca atttttaact tgttagggta taccctaaga ccccgaaata cattcaaagg
    11821 aggatttacc tatggcaaat agaatacgaa atgaaagact ggaaattaaa ctaactgaag
    11881 aagaaaaggc tctttttgag gagaaacgaa agctcgcaag gtgcagaaat atgagccact
    11941 ttatccgcaa atgcgttttg gaaaaggaaa tttatcaggt agatttagag cctttccgag
    12001 atttacaagg cttactttcc aatgcaacaa acaacatcaa tcagattgca aagcgtgtaa
    12061 attccactgg tgttatctat aaagatgaca taaatgatat aaaaaatgag attgaacatt
    12121 tctcaaaaga gctatggcaa attcattccc tgctccttaa cagaacttcc ggaggtgatt
    12181 aaatcttatg gctattacaa aaatacatcc tataaaatca acccttaatc tcgccatttc
    12241 atatatcgtt aatggagaaa aaacagatga gcaaatctta gtaagcactc ataaatgcca
    12301 tcaggaaact gctcataccc aatttttaag aacacgaaat gatgtcggaa caaacggaac
    12361 cgttcttgca agacacctta ttcaatcctt tttacccgga gaagcaagtc ctgaaatggc
    12421 acatcaaatc ggtatggagc tatgtaaaaa gatattaaag gacgaatacg aatttgtctt
    12481 atctacccac atagataaag ggcatatcca caaccatatt atcttcaata atgtaaatat
    12541 ggtaacagga aaatgctatc attccaacaa gaaaagctac caccaaatca ggtatcaaag
    12601 cgataagcta tgcaaagaaa ataacctttc tgtgattgat aagttctacg agagctataa
    12661 gagaaagtac aaaacaaatg gtaagtcatg gtatgaaaat gagcaatcta agaaaggtac
    12721 ttcttggaaa agcaaacttc aatttgacat tgatcgtctg ataaaacagt ctaaggattg
    12781 ggaagaattt ctaaagaaaa tggcggagct tggttatgaa ataaagtatg gaaaacatat
    12841 agcctttaag ccaaaagaca aacagagatt tacaagagct aagacgattg gtgaggacta
    12901 tacagaagaa agattaaaag agcgtattac agaaaatcaa acgattaaaa ccccacctgt
    12961 caaaaaacga atcggcaatg ttattgacat gaataccaat gtgaaagtaa aggaaagcaa
    13021 aggatacgaa tattgggcaa ccaaacataa ccttaataca atggctgagt ctgtaattta
    13081 tatcagagaa catggcatta aatccgttaa acagcttgat gagtacatcc aaaaagtagc
    13141 cgacgaaaga caaaatttac aggataaaat aaagattatt gataaagaaa tgcaggagct
    13201 ttctaccact atggaaaaag ttcataccgt taaaaaatac aagcaatact acatggaata
    13261 taaagcaaat ccatccgata aggcattttt tgaggagcat aaggctgaaa tcacacgcta
    13321 tgaaacggct cttgcaaaac ttaaaaagtc ctattcaaag cttcctgatt caaagggcat
    13381 tttagataaa cttgataaat tgcaagaaaa aaagaatacc ctaatgcaag agtattcttc
    13441 tacaaaatct actatggacg agctttatca aatacgaaag aactatggaa tttatatgga
    13501 taaggagatg gagagatagc catctcctta ttaacccctg ctatattaag catcgttcat
    13561 acattgtaaa agctcataat tctgaacata catctcacta tttagtaagc tatatttttc
    13621 attttctaaa acttttatat ttatattcga atcaatgcta taatcttttt ctgaaaatcc
    13681 tattgccgaa acaattaact ggctaaattg atctgatgat tccataatat actcgactaa
    13741 tgcctgcttc ttctcttgat ctgtctccgc attgttaggg ctatcaaaaa ccattggaaa
    13801 tgttgttcct tcaggattaa atttctgttt taaatcattg agtgttagat accatataac
    13861 ggttgataat ggtttgttgc ttccacttgc acaaaaattt ttagatagtt gttcataact
    13921 gtcagcttct aactcattta aattgaatct tatttttaat ttatcaatca aataatagta
    13981 attttcatca acagatttaa ttttatcttt aacactttta aattctttct ttagtggctt
    14041 tagagcctcc tttgtactgt ctataactct gctattaata gtcaaatcat tattaatctc
    14101 atcgattagt ttatttagtc ctctgaactt gatatagtct ttgatttcct ttttgttttt
    14161 acaaattttt ttattatgct cctccaaaac caaagttaag tttgaatata ttccctcatt
    14221 tttgagtatt tcatcttcaa tatgggcatt ttctgttttt attgcatctt tcagactaag
    14281 agcattatca atatgattat atctcttact ccttagttcc aaagttgaat ctaaaacaga
    14341 atgacattca ggacaaactt ttgattttaa aatagttttt atttcctttt cttcttttat
    14401 ttcgaattta gatatttgag ataaagtgat attctgttca actaattgat ttctcaagtc
    14461 aacaagctta ttccttacta aattcatctc attaattagc gatgaatatt tttgagtttc
    14521 tatattaagc tcattttcta atgcactgct tgtttccgga catgaaaaac catccaaaat
    14581 taaaatggtt ctgtctttca ttttaattag gtcttctatt tcttctttct tacttttaat
    14641 ttcaagttct aattcttctt tgtgtttcac aagttcgaaa tattctttat tgtaaatgcc
    14701 caaatgtgag taaataacat taagtttaaa attactgaac tgagcaaggt ttttaaacga
    14761 attaaatttt gtaccgatgt attcatcttg atctaaaaaa ttcagcaaaa aactatatgc
    14821 aggtggtgct attactaatt gatttgtatt tttttgtggc aaacatatag aaaaattaaa
    14881 taaatttcca aaaaaatctg ctaattcact tctatgaata gtagtaaaaa taagatcacc
    14941 ttttgcatta aatattttaa ataatttttg agatctataa atcgaaaaat ctttatcatc
    15001 caccttaaaa aataagatat agaccttatc cttttctccc cacttcgagt caaagtgaga
    15061 atccgcaccc aaggcatggt ataaacttct taataaaact gattttcctc tatctgttcc
    15121 gtctatatca ctcgatgtaa taatattaac tcctttttta aattctgttg aataagcctt
    15181 tttttttaca acatcaaata tatatatttt gtcaaatata acattcatta taaaattcct
    15241 cctgaagcat acatataata aatcattatg tattgaaccg atttcatatc atcagtaaat
    15301 tccacatcaa aatcgtcatc aaataatttt gatatttctt ttaaataact ttcaatatcg
    15361 tctaacgaat tttcatgttc cacaatataa ttataaattt ttatttttat taatcgtaaa
    15421 gactcacttt gctgtgtttc aagtatatta ccaagtgccg tattgtatct tcttcgttta
    15481 gcaaatgaca agttatttat atactcctgt gtttcattga ttccattttt agattccttt
    15541 ttatgtgcat ttaacatttt atcaaattca cttcttgtaa tacctttgtt ttttacaaca
    15601 tcttcataag taccggaatc aaattcataa gaagcttttt ctttaacaga atcaacaacc
    15661 agtctataca atgcattcgg attttgaggt tcttcacctt taatatcaac aaaagatttt
    15721 actaatttcc ctctaataga atcctctgga tttaataaat ccatgttatc aaatatataa
    15781 aacacattat ctaaacatac agtgtctaaa tttaattctg tacacaactt tttcctaaca
    15841 tcatcaagta catcctgatc taaatctcca aaacattgct caggaaaatc aatttcttta
    15901 ttattgattt taagttgttt attacacaca attgctaatt ttatattgta gttcggtgaa
    15961 tataaagcat atagtttccc taaaatagaa ttgttcttat tcttaccttt cttacacaaa
    16021 ttattcgaat tgtaattacc actttttttc gtcttgattt gataaaaatc caattcatcc
    16081 tctttatgca attctatatc acatttaaaa tcaaaaatta tcgtatattc attgttcgct
    16141 ttatgttcat cgattatctt actgattccc cataataatt ctaatctaaa gcgattttta
    16201 gcaatgcttc ctgataaatc ttttggtaag ttcatgtagt acttttcttt agacatactt
    16261 aacctccttt caaaatctat ttatcaattc ttatgcactc cacgatttcc tcaatatcac
    16321 aatccaaagc gttacatatc ctaagcaaaa catccgtagt tacattctgc ccgtttttca
    16381 ttttataaaa tgtactttta ctcacttttg ccttttccat caattcatta tttgtcatat
    16441 ctaaatcaat gagttttttg aacaatcttt tataactaaa agccctcatt cttatcctcc
    16501 gctcaagaaa actatattta gtatattata ccataaaata tacttttttc ataaatatat
    16561 tctctttaat cgaactcgtg caaataaaaa aagagtcggt gcagtcctaa gaccacaccg
    16621 accataaatt tatctctcag cttcttggtt tgcctctttc ttttcttttg gcttttcctt
    16681 atcttcagcc tgatacttct taatagctcc aagtacagat tctttctttt cttcttgtcc
    16741 tttcatttgt tccttttcct ttaacagttt tgaagaaagt attctgatat tcctatgttc
    16801 cttgccgtta tcatcaatag atgttcttat ttgtccaaag agcttaacaa aatctccctg
    16861 cttaaagtcc tttggaatat cgctttttcc cccataagcg gagcaattat gatatacctt
    16921 attcccctca tcgtctttgg atactacgga gaaattcgcc accttaaagg cttctccatt
    16981 cttgttttcc ctttctacta catcaacctc tccaacgata tttccaacaa tattgataag
    17041 attatccttt tcttcctgaa cataaggcag gtcttttatc tgtccctgtt cccttaaatc
    17101 ctcaatcata tagtcaaatt cctcatgcag taaattaagg ctgtcattgt ccatataagc
    17161 gtcatagagc ttgtctaaag cactttcatc attgatacct ttttccatgc ttataatcgc
    17221 ctttacaaag tccttatggt tataatttac catatcttct atttgattta tcattgtttt
    17281 gtaatccatg tttttatctc ctttcatggc aaaagggagg ttgccctccc cttatcttaa
    17341 aatttcctgt tcttttgttt tttgtttttc ttctgtttta ctttcatttt gataagctcg
    17401 tatctgactt aaaatagacg gcttatcctc tgctttcgtt acagattcca cttttgtatt
    17461 taagttatcc tccacatcat aagtggactc aaaataatcg ctgtctctaa agtcattgtc
    17521 gtacctatca gcaataccat cattatcaag gtctttagaa agcggatcat agaagttacc
    17581 ctcatcatca atatcaagtc caagagcagc tttcaagtcc tctgaatcta catagaccaa
    17641 atcattgaaa gagaatagct caatctcatt tttcatattc ctaagagctt cattttctcc
    17701 tttattctca taataaaagc tctctgtctt gattggcgta ccatcaatat actgtgtcca
    17761 agttttctcc tctaaatcaa gctcatactg aataccatgc ctttcatccg gtgtattggt
    17821 ataggcgata ccgatatgct ttaagtcagg atacaaggta ttaaattcat catagctatg
    17881 attttcttcg tactctctgt tacagaaatc aatgattgcc atttttacat cttctacaag
    17941 tggattatca tttctctttt cttccacttc tcccatatca aggagcttat tcagttctgc
    18001 aagtcttaac accttagttt tcagctcatc tgccttctca aaaggtttct ttaattcttc
    18061 tttggcattt tgaagttgtt cctttgtgct gataagtttt tcttcaagcc tatttaattt
    18121 ctcaggcatt ttctcaagag cattatccag tcttgtaatg ttaccgtccg cacttgttcc
    18181 aagctctcct gaatgctttg cagcaccgtt taggctaaaa ttatgttcat tggtaaagaa
    18241 gttatagctt acctctaaat ccatatttct atacttgcct atcaccttgc tttcattgat
    18301 tttcactttg gaaatagctt caagcagtct ttctccagct aatttcttat cgttaatctt
    18361 ttctcccgca atggtaatgg aagtaaactt ttcctcacct tccgctttag gttctacact
    18421 tgctatatct ttcttaacag cttctatgag tttttccgtt cttgcgattt cttcaggata
    18481 gtttttcgcc actttatcct ctaatctgta acggttggac ttatagtttg cttcaagcat
    18541 tttaagtttc gtaacctcgt tatccaagtc catcttttcc ttgatttttg gatcaccagt
    18601 ggcaagtgct ttaatctctg catagttaag ggagctttcg tccacatctt ctgcaactct
    18661 gacgggagtc ttactggtca taatctgtga aatgaacttc tgcttatttt ctatcgtctg
    18721 ccaaaggtac gcatcaaagg tattctctgt aacataacga taaatattta ctttctcatt
    18781 ttcgtttccc tgtcttacaa ttctaccagc tctttgttca aggtcagctg gtctccaagg
    18841 cacatccaaa tcatgaagtg caatcagttt attttgcaca ttcgttccgg ctcccatctt
    18901 ctgcgttgaa cccattaaga tacgaacttc gccttttctt acctttgcaa agagttcatc
    18961 cttttgctta tcagaattcg cttcatggat aaaggcgatt tcttctttcg gtattcccat
    19021 tgctacaagt ttttctctaa tatcatcata gatattaaat tctccatctc cttttggagt
    19081 agacatatcg gagaaaagta gctgtgttga cctattttct tttgtcttat cccaaatagc
    19141 aaatacattt tttacgcata catttacttt actatcaggg ttatcaggaa gtaggggatt
    19201 gattaaacgc tgatctaagg caagtttctt accgtcattg gtaatcttaa gcatattatc
    19261 aacatccggc tctacaaccc tatttctgac atcatccgct ctttcagaaa ggctctttag
    19321 gatttccttt tgttcttcag aaggctcagt tttaataact tcataattag cttcaggtgt
    19381 aggaaggtta agcatatccg cagtctgaat atctgctact tccttgaaca tactcattag
    19441 ttcaggcaag ttatagaact ttgaaaatct cgtttttacc ctatatcctg ttccttcagg
    19501 agatagttca aaggagcttt gtgtttctcc aaaagtggaa gcccaagaat caaaatgctc
    19561 taagtgattt ttctttaaac tctcatactg aagatacctt tgcattgtat aaagctctgt
    19621 cattgagtta cttacaggtg ttcctgtggc aaaaactatg ccttttcctc ctgtcatttc
    19681 atccatatat ctgcatttca taaacatatc ggaggactta aaggcttctg actgcccgat
    19741 acctgcaaca tttctcattt tggtatagag ataaaggttc ttgtaattat gtgcctcgtc
    19801 aacaaagagc atatcaactc ccagttcctc aaaggtaatt atatcgtctt tcttaaaatc
    19861 atcatttaat ttctcaagcc ttgtttccag ttttttcttt gtcttttcaa gctgttttac
    19921 tgtaaagttc tgatttctgt catgcttgta ttcttctaca tagtttacga tttcattaat
    19981 ctgatcttgg atgtgctttt cctgatattc cttactcatc gggatttttt caaactgcgt
    20041 atgcccgata acaaccgcat catattcccc tgtggcaatc tttccgataa atctctttct
    20101 gttcttcggt tcaaagtctt ttttatcagc caccataata ttagccgacg gatatagctg
    20161 cataaattca cgaccgattt gccctgttaa gtgatttgga acaacaaaca aagacttact
    20221 gcacattcca agtcttttac tttccattgc ggaagctacc atctcaaagg tctttccact
    20281 gcctaccaca tgagcaagca atgtgtttcc tccataaaga cttcttgcta tggcatttct
    20341 ttgatgaggt cttaaatcaa tttctgtatt cattccctca aaagaaagat tgcttccatc
    20401 atattctctg tttcggattg agttaaaacg ctcattatac agttttacaa gacggtttct
    20461 tctttcctga tcgttaaata tccagttctt aaattcttct tttaatagct cctgcttttg
    20521 tcctgcaagc atggtttctt ttttatttaa gacagaagtt ttagaaccgt ccggatttat
    20581 aatctggtca aatacctttg tttctttaag atttagggca tcttcaatca gcttataggc
    20641 atttaccctt gatgtaccaa aggtcatttc tgcaaggtca tttcctttat ccctgctttt
    20701 tccctctaca ttccattcac ttgtaatatt tgaaaattta accttaatat cccatcttgc
    20761 atatcccgga gtttttaaag tttcaaagat gaatttttca atatctttaa tcggtatcca
    20821 agtcgcacca agacgcacat tgatttcact tgcttctaat tcctttggaa gaactttggt
    20881 aagctctgct ttttgatatt ccaaacgatt catttcatag cttatcagct ctttttcttt
    20941 tccattctca acaaagccaa gatgaggaag ctctcgttcc gtttgtctta actttgataa
    21001 atagctatct acaatggcaa tcttatccct gatattccca cttaggtatt catcttttgt
    21061 tacatagcca tatttatagg aattactgcc atttgcacag gcaaaaggta aatctccatc
    21121 ttcaggatta aaagaaagtg gtctgtaaaa attttgttct tctctgatat ttaagtagat
    21181 ttcccctcga agctcctcaa ttaaagtcgc tctgtctttt cctgtaagac ttcccatata
    21241 gtcaaagtcc acatagcctt tttcagatac cgataaaaca agggcttcca aagaagtatc
    21301 cacatggtct ataactttgg cttttgtaat agttcttttg gagaaaatat ctccctttgc
    21361 cttgaaattt tcttcttcat caagaatttc tatggaggaa acaagtggaa agttgctatc
    21421 ctctttcaaa gctctggtat tacttaaatt attgacaaag ccatgcttct ttgaaaagtt
    21481 gtcatatact tcatttagtt tttcctgtga agccttgatt tcttcttcgc taaaatcttc
    21541 cttctgcttg taaatcacat cttttaaggc aacattcagc tcaagataat ctttgatttt
    21601 ttctttattc ttgtctgata cttccttttt aataaagagc gaattttctc tgtaatagac
    21661 ttcatcgtca ttcaaggtat aagagaagtt ctttacatca tcggttgcag ggattgaagt
    21721 gatttcatca tctaaaagct ctatttcttc atacttggta tcttttgaaa ttctttctcc
    21781 tgcaatctcg atacgctcct ttaatgaagc catatttatc tcttgcccta aataagctat
    21841 cggttcacag gttagagttt ttccaaacct tccgcttact tcacgcatag aaccaagcac
    21901 ctgctcagga tgatcgacaa aatatttgtt atatacaagc ccgtttttat cttctgaaag
    21961 gtgtatccaa gcttcatctc gctctaatac gctatctctt ttctttagga atataatatc
    22021 gcttgttacc tcagttccgg caacaccctt aaaggtatca tttggaagtc tgattgctcc
    22081 taaaaattca gctcttgctg caagatacct tcttacactt tcgtcctttt tatccattgt
    22141 tctagatgat gtaataaagg tgataacacc gccgttacga accttatcaa tggacttggc
    22201 aaagaaataa tcgtgaataa gaaagttatt gcgattgtat tctctgtcat ttaccttgta
    22261 ctctccaaat gggacatttc cgattgctac atcaaagaag ttattggaga atcccgtttc
    22321 ttcaagtccc tttacctgta cttcactttc cggatacagt agttttccaa tgcgaccact
    22381 taccgagtca agctctactc cataaaactt tgacttaccc atttcatcag gtacattacc
    22441 gataaagttt ccgataccca ttgacggttc aaggatatta ccttgtttaa atcccatacc
    22501 ggaaagagtc ttatatattc catctataac tattttcggt gtataaaagc tcgttagggt
    22561 tgattctctt gcagcttcat attctgatga cgatagattt tcctttaaga aggctcttgc
    22621 atctttccac tgtccatcct tgctttcatc aaagacatca gaaagtccac cccagccgac
    22681 atatttagct aaaacttcct gagctgtaat atcaagctct cgttctccac tttccactct
    22741 gttaagcata gagattgctt caaggttatt atttaacctc tcacttggag ataacctttg
    22801 aggaagtgtt tcttctgtaa tcttgaaatt gaatacttct tgtttaggct gaatatcaat
    22861 ctttttttct tctaattgtg ttaatacttg cttgatgatt tccgtatctt caacatcttc
    22921 ttcaatcatc tcattcaaac tatttatata tactgaaaca tttcctgctt gtaaatcacc
    22981 atagatttgg ttgatattat caagtctttt ctctgattct gcttctacgg aatccgcata
    23041 gtcataagaa tcaaaatcat aagctatctc atctattctt actgcaaggt cataaagctc
    23101 ctctttttta taggtcaaat tttcaaaaat tttattaagc tcactttctt ggcgataagg
    23161 aattacatcg gagcctgtaa tcatacctcc aagatattcg gtattatcct taaccgttac
    23221 agtctttagg ttatttccca tattatcaaa ttttgtgatg gcatagtctt ttcccttgta
    23281 cttaacttca tcaccgatga taaattcagg tctttcaaga cttatttgtc ttaataaatc
    23341 ctcattatct gtaaaagcta caatagggat ttgatgatta ccttgcctta caggatcaag
    23401 ccacaaatca ttttttcctg tgatttgatt tttagaaaga tctcttactt tgtactcctc
    23461 atgattaaaa tagacggtat cgccttcttt aactgtaaac tcatctgaaa ggctgttctc
    23521 tttttcgtta agctctactt cttctttttc ctttagatag tcaaataaag tagcctgaaa
    23581 aggttctgct tgtttgcgtt cttctacatc ttcaggtgct tcaagttcta tattattttc
    23641 ctcaaaactt agctctccgt taaaaacttg ataaagctcc tgctcatcca tctgcttttt
    23701 aagttcactg tctttaagtt ctctaaaggt ctttggaaaa tcttctaaaa caagcctctg
    23761 ggcatttaat tcctttagct catcaaggtt aaaatatccc cattccggct caattcctaa
    23821 aacaagtcca aaagcatctt tactttccct gtcatattcc gtcatatacc aagtccagtt
    23881 acttctaaat ggaatgatgt atgctgcatg aacctgtttg tctgctaaag ctacatcctc
    23941 ttgtgcataa agttcaggaa ctttttcaag catttcatca gtaattagat tatatggatc
    24001 gtctttagag taataacttg gctctttttg ttcttctatc tctacctttt tttcaatgtt
    24061 cttttcttca agataaagat tcttagaaag cagctcatct atatgctttg ctacatttga
    24121 ccatgtaaga aaaacatcgt tacaatgatt cttctgtaat ttcagtccct ttgcatcatg
    24181 ccattctccg cttcctcttt cgcctgatac agcatgagag cctccaccga tcccatattc
    24241 accttttagg aaattcgctt tttcctgcaa ggtatgattt tctttgaaaa acttagtaat
    24301 tcgctctttt cctttatcga ctccacttcc tcttgaaatt gtagcaagga cttcatcttc
    24361 tgtaataaag ccctgtatct ttggaagttc gataaggtta gaggtatatt cctttcttgt
    24421 aagtgcaagc tcctgtaatc tctggtaaag gctatctact ttgtgatagt gaaatcttag
    24481 tacctctttg ttttctttat atcctgtcaa aaagcgactg tactcggaaa caacatcttt
    24541 tagatagtca ggatttttaa gagcttccga taactgtctt gtttctttcg gaaagccacc
    24601 acctgtttct ataaagtcaa aatagccctg tgctttccct tcttcgctta aatcgtgata
    24661 tagataccat agcgattctg aaactctatt tctttcatag tcctgtgctt ctaaaagctc
    24721 tacatttgta gcaaactctc cattttccaa aagctcattg attcttcttg cagcatcact
    24781 ccagcttaaa atttgcgtca tatcttctct tgcggaagtt ccgtaggcta aatgaatgcc
    24841 tttatccgaa taccaggaag atacttcagt tccattaagg aagaaaccgt ttccgcctcc
    24901 catggtatct ttaaggtatt ctcctaattt ttctactgtt tttcctttgg aaaattcagc
    24961 aatgatagga agtctgccac cctcatggtt tccaccgttt acaagaatgc tgtcaatatc
    25021 attttgggca agaggtattg taagtttaat ttgtcctgag ctgttttcag gcaaggaaaa
    25081 agaagcctta tcagcttccc ttatctcttt atccgtattc tcttttagat ttccactact
    25141 tccttgattg tcatttcttt gagggctgaa atcattgctt tgtactgagg atggctctcg
    25201 ttctctatct gccaagacat catcagtttt tgcttttcgg ctctcataaa ctctatcacc
    25261 tgtttctgag tatccatcag gtgtttggtc agcttcttct ccttgtatag atccactaac
    25321 atctcgaagt ggctctgctc ttcgctgttt gaaagataat tcagcctcat cactgcgtac
    25381 actggattcg gatattccct cataaagtcc atctcttcct ctaactgatt cagtgtattc
    25441 tccttgattt tctctattat ctcgcctgta ctttccattt cggaaaattc gctcgttttc
    25501 actaattctt tcctgatcat ctcgtcgaag tacattttct tctacctcct ctaattcttc
    25561 ttttatttta ttatatcctg cttctttacc tcttaaaact tcttttttaa gctctaactc
    25621 tttgcttttt tgaatagtcg tatcaataac tgttccgcta atatccgaca ccgtttctcc
    25681 aaggctcata agagaaatgc tgtcaagcat tgcaaagttt tcctttaaca gctccttatc
    25741 cataggatag tctaacttaa atcttgaagc tactgcatag cttatcgaat ccctcacaaa
    25801 tttagtaaag gaaattctat cctcgtcagc tattctaagc tcattcatca ggttatctat
    25861 tttttcatct ccgtagagcc tgcttaaaga aaagatattt tctaaggtgc tttcgctttc
    25921 ttcatagcct tcagttttta tcatttcttt taatacatct ttgtgagctt ccttatcaaa
    25981 tctccaaaga ttaacctcgt tgacatccct attctttgaa actgtctggc ttatatcaaa
    26041 gatatagtca acttttttgt atgtgccgta gtcctctaaa atagggatac ccttttgacc
    26101 tcgcattact gttcggttaa agcgttctct ccagtagtcg aacttggcac aggctgtggc
    26161 ttcaggattt ttatcgtaga tactaagttg acttctaaag tcatatctct gattgtttcc
    26221 gacaaccttt aagagcttca gatattctgc ttcactttgc agaatatcct gttttacaag
    26281 ctccaaaatg ttatgaaaat catttattcg catatttcct ccttcttttt tacaaataaa
    26341 aaaggcaatt agattttctc taaccacctt ttgattctat atattatacg ttgaatacaa
    26401 gtaataagaa ctaattgaat agaatttaat taaacctcaa atgtataacc aatgttcgat
    26461 gcaacttatc tgcaaaatac atatataacc tcgtcattag ttttaatccc tgacaagcct
    26521 tcaatgctcc atgaaaaaat ccatcaactt ccaatagtat tgttccagtc ttatctgcta
    26581 aatctttaga attatcaaca ctaaaataca tttgtgcatt tttatttcct gttttcttca
    26641 catatttatt agcaagcttc aaacctttag agtttgttgc gtcaaatatc aattctccct
    26701 tagaaaattg agctttcaat ttttgaatca tatctataat tttatattcg tcaaaatact
    26761 gatatacacc cgctacagaa ataagtgtcg gcaatgaagt atctatttct ttaatccagt
    26821 taaggtcaaa catatctcct gatataagtt tctcattttt agcatttcca agcactcttt
    26881 ttcgtacttc tattacaccc ggaagatcta cttgatagaa atttacaatt tgatttccta
    26941 tgcgattata tgaagtctct aaacctgctc ctaagaatac cacatttccc tttttatttt
    27001 catcaaggaa ttttttaatt ttcatatcta tggtctgttg tctacaaaca ctagccatat
    27061 aaaaatattc cgcactattg ttatctatac cgcctgttgg aatcttgggt ttcaaagata
    27121 gagccttttc atcatagaaa aaatgtggga atttttcaga tacatatatt ctgacagcta
    27181 aaggtatata taaagtatcc tcaacaccgt taaattcatt gcctgtacgc atttcttttc
    27241 atatcctttc atagtatatt actaaattga aacactttta ttttattatt gtttccacat
    27301 ggactacttc actcattttt tgatgcacaa agttatattt atctattttt catcatgtct
    27361 ttctatctcc tgctctgcaa ttcctgtata ctcttttaac tttctttgta atagttcttt
    27421 atctatcaat tttaatctat attcagatac cattgttggc gaaaggcttc tgcttagtgc
    27481 aaactcaact acttcatcat cttttgatgc acaaagaata actccaacac ttgggttttc
    27541 cgcttgtttc ttaacttctc tatctaaagc ttctagatat aggttcattt taccaacata
    27601 ttccggctta aattctccga ttttaagttc aaaagcaacc aagcaggata aacctctatg
    27661 gtaaaataat aaatcaatat agtagtcatg atttccaacc tgcactctat actcattacc
    27721 tataaaagaa aaatcttttc caatttctaa aacaaagttc ttcaaatttt caagaattga
    27781 cttctgaaag tctctttcat ttccaatttt aggagcatca agaaattcta atacatagct
    27841 atccataaac aaattatgtg tttcctgttt cgctctttgt attgcaggca tatttccctc
    27901 atttgaaagc atacagcgtt catagtatcc actatccatc tgtcttacca gctctcgatg
    27961 tgtcaaattt tccttaattg ctaagttaat ataaaattct ctctcttctc tacttttagc
    28021 tcccgacatt atttttaaat gattagacca actcaattgt gtcaacagcg ttgacacttt
    28081 ttcatcatct ttgtacagtt cgtaaaattg cttcattcta taaaggcctc tgcgattaaa
    28141 tccttttaaa tcaggatagt taatagaaaa aaattcagct acattatcta cgaatcctga
    28201 accataagat gcttcctttg ttttttcact aatatatttc ccaacctctt gatacattaa
    28261 gattagctct tcattaactt ttctatatgc acgatccttt gcattttcta caatcataac
    28321 aatttcttca aatgaattgt tgtttttttc tatgtttata ttttgtaaag aattcttttc
    28381 tttattcata aattcctcct atctgcatct gccatctata atttatttcc tgccctgtat
    28441 atttttctct ataaaagttt ttgaattaac aatttgtatt ttcccttcag ttacaacaaa
    28501 agttacataa tctccattct cgatttctaa tatttctctt attttctttg gaatagtaac
    28561 ctgtcccttt gccataacct ttgcattatt cataaatact tccgacataa aacacctcca
    28621 tatttcctac tttccctaca aaaattatag catttttagg acattttttc gatattaaaa
    28681 ttagtgctat cttacttgct ataagttgaa accaattctt ttatctcgtt ttcaatcgct
    28741 tttaaaaaat tttcctttga agctttgcct tgtgcaatat ccgataactc catttcccac
    28801 ttcgcggttg tacctgctga tttaaaagta tctgagacaa tcgttacaag gcttattcct
    28861 ttgtgtgtag caataaggtt tttcttatct ctttctacaa aacccttaaa gattaaattt
    28921 tcaataatgc ctgctcttgt tgcaggtgtt ccaagtcctt ttcgctcaac ttctacacct
    28981 ttttccagtg cgtcatttcc tgcaagctcc atatccttta aaagtgtatc ttccgtaaaa
    29041 tgtttagccg gtgatgtgta tttttctttt atctctttct ccttaatatc aagactatca
    29101 ccaatcttta aatccggaaa cacggtatct tcatttttct tttttatata tttttttagg
    29161 tatttggtaa agccctcatc tacaatcacc tttccactgc ttgtaaattc aaatccgtca
    29221 aatacagcta caatctttgt agtattctca attaaaggat agcctacact tgcatgtagc
    29281 ttatttaaaa caagttcata taccttcaat tcactttccg gaagtgaagc taagtcctca
    29341 ttcatactgc ttgctgtcgg tataatcgca tgatggtctg taaccctttt agaattaaat
    29401 accatcttaa tgcgacctgt atcaaaatca tgctttccaa taatattgtt gattgtactt
    29461 gtaatcatat cttctgtaag ataacgactg tccgttcttg gataggtaat cagttttttc
    29521 tcgtataaac tttgtgtata atctaaggtc tgctttgcac tataatttaa atacttatta
    29581 cactctcttt gaagcgttgt aaggtcaaat ggcagctcag gttttgttat cttttccttt
    29641 tgaataacat cggtaatttc tatggaatta cctaatagat tgataagctg ctctgtaact
    29701 gtcttatcat caattctatc ggtagaaaga gaaaagccgt ctaaatcaag ctctactgta
    29761 aaatattttt ctttcttgaa atttgctatt tcatcatctc tttttacaat catggcaaga
    29821 gttggtgttt gtactctgcc aacagaataa ttctgcttat ataggcacga gtatagccta
    29881 cttaaattca ttcccacaag ccaatccgca atcgctcttg cttgtgcaga ttcaaagaga
    29941 ttatcgtaaa aacttccgtc ttttaggtta tcaaagccct ctttaattgc actatcttcc
    30001 attgatgaaa tccaaaggcg tttcatcttc tttttgcaat ttgcctgatt atagacaagc
    30061 ctgaagattg cttctccttc acgtcctgca tcgcaagcat ttatcacaat gtctatttcc
    30121 ttgtcattca tcaacttttt aagaatcgta aattgtttct ttgtggactt tgcaatctca
    30181 tacttatatt ctcttggaat aatcggtaaa tccgccatat tccatttagc gtacttttca
    30241 tcgtaacttt ccggatttgc catctgaatt aagtgtccaa cacaccaact taccttgtat
    30301 ccgtttccct cataatatcc gtctttcttt tttgttgctc caattacttt agcaatagaa
    30361 attgcaacac ttggcttttc tgctattaca agtttcatat agttttcctc ctgttttcaa
    30421 tataagaggg cgaaagattt tactctctcg ccctgtgttt tgccccaaat attatataat
    30481 ttgggggaca agtttactct atctcatcat cttctttatc ttctgttact gcttcgttta
    30541 cttcattttc gctttcttct gcctctgaaa agaaatcatc atcttcctcc tcaaagcctt
    30601 caagctcctt atcttctttt ttcttaataa ccttaaaata atatcctgca cctaaggctc
    30661 ccgctactac aagaatgaga attaagtagg ttcctgtatt actgctttct tcttttttca
    30721 cttgtttagg ctcttctttt tgtgattctt ctttaacaat ctctttctcc ggtgctttct
    30781 tttctaccat atttaagaga tcatcttcag atacttcagt taggagcata acattctcgc
    30841 tatcctcatc atggttaatg attaaatgaa aggtcttacc atttttagtt gtaaaggtaa
    30901 caaactgtct tgcatcagca gaatatttat ccgtttcttt attatcttta ctgtcattat
    30961 ggtgaatagg ataatttttg tttgcattat ccttattttc agtaactgtt cctcgtgcct
    31021 ttgaaggttc tgaagcaact cctttgttgg tatttatatt cttacttgaa ccatccagag
    31081 aagaatcgtg actaccatta ttagcaggct gttttggtgt cagcttattt ggataacgaa
    31141 cttgcttttc tttctccttt gattcattgc tgtttgtatc ttttgtattt gcccctttac
    31201 tttcctgcgt ggcagaacct ttaccactgc tatttataga tgtttgtggt gatacatttc
    31261 ctgttccttg tttaattccg gaaatagggc taattggtgt tacattagga ttatgcataa
    31321 taggagctct accgacctca ttagcctttt tctcaagttc cttaatctta tcggttagct
    31381 tgtctatctc ctttttcata tctgctgata agtccttatc atctttgccc ttttcaagtt
    31441 tttccttcag tttatctatt ttctcctcaa ggtctttgat tttatccttt tgcttctcgc
    31501 ttagtttatc cttgtcctta atctcctgat ttagcttatc aagtttttct ttaagttcct
    31561 tagactccct ttccatttta gaaatatcat ctttgctaag ctctgtttga gttcccttat
    31621 tttctatctt tgaagcattt gattgagagc tttgctcaga ttttgagctt tcattttctg
    31681 tttgcgtttc cttatcctta acatcaatct ttgtaacttt acggataatt tcatctttcc
    31741 cgtctccctt tacttcataa aatagaaatt catctataaa tttcatatca tcaggaatta
    31801 ttggaagatc tccactgtca agaagccacc ccttttcagc cttaatcttt tcctccttaa
    31861 agactttttc atcttcaaaa atatacttaa cattaacttc tacttctatt tgagcaacgg
    31921 cttctatcgg atttacaagt gtcgtaaaca accctccttg tgcaaataca atcttactac
    31981 ctgcaaataa agagattact actcccacta tcaaaagcac tgctccaaga attactcccg
    32041 cagccttttt attcttcatt tttttcattt ccgttctcct ttttcatttc tgatttttct
    32101 ctgtttactt ttgccataag atcagaaatt gtaatgttat tttttctgca aatggcaata
    32161 atttcttcgt tttcaagttc ttcttcacga atgaacaaag gctccaattc ttcatcaatc
    32221 agagcctttt tatctttcaa tttctttatt ctatttttta ctgctgtcag ttcttttttc
    32281 aaattgtatc ctcctatctt ggtcttccaa acccataaaa atgttctctc caatatggtg
    32341 agttaataga agtatattga ataggatccc ctgcgtgtat catcatccca tttccggcat
    32401 atatacctac atgagaaatc gggcttccac tattataggt atttttaaag aatattatat
    32461 ctcctgcctt ggcttcacct gtacttacag gattacagta atccgtataa attctccaag
    32521 ctgttgttct tggcatattt ttaatacctg aatgagtaaa gctccaacac acaaatgaag
    32581 agcagtcaaa atttgacggc ccatttgcac caaaaacata cctctttcct atatgtcttt
    32641 ctgcttctga gaaaagtcgt ttaaccgttt cttcattgaa agctattccc ggattgccaa
    32701 aattaggatt gtttattatt tcagaataat cattacttcc acttccaaaa aagtcactca
    32761 tattaccaag acttgcaagt aatgcttcat aatgtttcac attatctgga taattttgaa
    32821 acacttctcg aacaacacta tccatttctc ttttataaag agttacaatg agtttcttgt
    32881 actcataagg ttcttcatgg ctttcggtat attcgtttcc atcttcatca gtatagattt
    32941 ctgtaactgt cctgtatctg atttcaattt cttccttata ctcaagccta tacatggact
    33001 taaaaagctc atctaataca gagcttactt ctgatacatt ctttacttct ccgaaccttg
    33061 atgtaatgta ggataaaagc tcatgggtat tatgaccaat atctccctct ttattgacaa
    33121 tatactcatc ataaccggga taattttctt caacactttc aagctcatct tgtaatcctt
    33181 gctccatact agaaaattgt tgatgaattt ctgtgagaac attaggcttt gataaataac
    33241 ttgttgtaag aactgtactt gtagagttca taaagcctgt cattcctgtc cctgcaaagt
    33301 taataaagaa agttcccaaa agaataagac ctatgaaaat aatcataagt cccttcgttt
    33361 ttcttaatat aacttccttt gaacttttaa gactgccgat aagcccttcc ttaatgcgat
    33421 ctctaagcct tgacttattc tgccttttaa tggaatcttt catctgcttt cttttttgaa
    33481 atcgtttaaa gttatttgct ttttgatact cctccgtctt tttcagttcc tctttggcat
    33541 ctcgaaactc taatttggat tttcgctttc tgattttata gtccttattt gtaaggtcat
    33601 atcctttctt tgttcttttc ttatccgagt agttcttaat gccatgaatg agttttgagc
    33661 ttgtatctgc tgtcttttct cctgcctcta ccccttgatt ttcatcactt ccatgagata
    33721 aataatctct tacggtttcg cttccttttg ctaagcctga taaagcagat actttagaag
    33781 tcttattctc gaaaattgtt tcctgttctt tagaaagcct gtttttcttt tctctatcaa
    33841 gaacggcttc ttttccgctt tttttattat ctgctttttc ctctttagct tctttgtcct
    33901 tctttcttgt atagagctta tcggaatagt tttttctctt ataatttttc ttttgagtgt
    33961 tttgcttatt taccggattt ccgctaatct catctttttt taatccatct acatcatagg
    34021 ttgactcaaa atagtcgctg tccctaaaat cattgtcata tctatctatg attccgtcat
    34081 tatcaaggtc ttttcctaaa ggatcataga tttttccatc cttaacctcc gtaccataat
    34141 ccgctttccc gatttcttcc tgacttacac ctccagtttc agttgaagaa gttatgctat
    34201 ccttattgga agctctatat cttgcatttc ttttggaagt ccctctaact ggttcactgt
    34261 cagaggtcat tttgcttgtt ttctcatgca ccttatcttg gaatctgtct ttatcatgaa
    34321 caattttccc tctgtaatca tcgttatact ttaacttact ttcttcaggc tcaattccca
    34381 tattactttg cagcgtttcc ttttcaatcc ttgccctttg cctttcctta aagtcctttt
    34441 tcagtttctt tcccataagc ctacctcact tcttcaggct tggttgtcat cttctgataa
    34501 agtattgtgt cttttgggaa cttatcaagg aaaggaacaa tggtgtttcc aaagaacaaa
    34561 agtccttctc ctgcattgga attagttaca tattttagct gtggctgaga gattttaagt
    34621 ttccttgcta aaatttctct atcgcctgaa gcctgattaa gcattaaaac aaagtccgta
    34681 ttatcaaaga tattttcaat ttcattactc ataagtaagt ctttgacatt ttgtgtaatg
    34741 cctgtcggga ttccacccca cttacgaaat cttttccaaa tctctacgga ataggaagct
    34801 gtctgctcgt cttttaacaa taggtgaaac tcgtctatat aataccttgt agccttattt
    34861 cctctgtttt gcgatacctt gttccacacc tgatcctgaa taacaagcat tccaattttt
    34921 ttaagctgcg agcctaactc cttaatatca aaacaaagaa gctgcttatt taagtccaca
    34981 tttgacctgt tattaaatac attaagagat cctgttacat aaatctccat ttctgttgcc
    35041 aactttttac ctactttttc ttcctgattt tttaacatat catacaggtc ttgaagaata
    35101 ggcatattct caagaattgg attttcaaaa tactttccat aaatcttagg cagacatcta
    35161 tctataaccg acttttcttc tgctgtaaga ccactacctc ctacaacaag ctcaagcata
    35221 gacataatga agtttgcttt gtcctttagt ggtgcgtcac catctccata gttcatattt
    35281 atatctaaag gattgagata gtccttcgac ttactgctga cctttatgac ttttccctta
    35341 aattgtctta caaggtttcc gtattcgcct tccggatcac agataatcac atcgtcatcg
    35401 gttgtaagaa tggcatttgc catttctctt tttgcactaa aggatttacc acttcccgga
    35461 gttccaagga ttagaccgtt cgggtttttt aacttctttc tatctgccat aatcaggtta
    35521 tgacttaggg catttagtcc atagtacaaa ctgtttgccg agtttatgaa aagctcctct
    35581 gtggtaaacg gcataaagac cgccgtagat gatgaggtta gccctctatc aatttcaatc
    35641 ttatttaccc caagaggaag tacactgatt aagccctgct cctgtgagtg gtcaagcctt
    35701 tttaacttgc aattatgctt attggcaatg gagctaattt gagaaatggt gttatccagc
    35761 ttttgaattg tccttgcaaa gttcataaaa atgatggtta caacaaacat tctctcatct
    35821 cttgtctgta agtcttttaa taggcttttc acatcttccc cataggtaat taaatcactt
    35881 ggaagaatgt ccatgtcata ccctgacctt acagcctttt tgttttcctc aatcttcatc
    35941 ttgtcaatat cggtattttt tcttttgacc atcttgatgg cttctgactg ctcaattgcc
    36001 ttgatatgaa aagagatatt gatgttatca tctatatcca aaaactctga cagcatacga
    36061 tctgaaagct cacttgcaag aatttgaaaa tgacttgctg ctccgataaa atttccaaac
    36121 ttaaaatacc ttgacggtgt aaagttaaat tcatctggag caatatatgt ctttgtactt
    36181 tcctttttct ttaagtcctt atatgaaaat tcaaaggtct tattcggatt taaaacatca
    36241 tgaagtatct ttagcctttc ctccccgtta aggctttctg ctcttactcc catactttta
    36301 aagcttgata atatatctat ctccattctt tccagttttg atgttgcctg ttctaaatta
    36361 tccgcttcta ctgtaaaagt tacatatttt gattttttca gtccgttatt tccttttaca
    36421 atctggcttt taagcatttc tcgaaactca agacgtatat cgtcaaaacc atctccttta
    36481 tccggtattt gaattgccga tttcatttct tcatttctgc caagctgatt gatatatgaa
    36541 aactctatgc ttatacttgg atcaaaggaa ttaagaaagt ttgcaaactg attaaaaatc
    36601 aaatccctat cttcttctaa agcaagctga tagtttatat cctcaaaggc gatactctta
    36661 ttaaaatgat tttcatcaat ctgacatatc ccacttttta atagtctaag gtagggaatg
    36721 gtatcttcaa cagtatatct ttttggttct ttcctaaaga tagggtcaag tagcccacct
    36781 ttatcttttt tagattttcc cttattttgc tttaagtcct gtctttgact tcttaacttc
    36841 tttttgtttt gttctaactt tagctgctga atctttcttt ttttgttcaa ggtaaacctc
    36901 ctttctcact cttttttgtg gctgataaaa cttatgaagg taaatatatt taaaatattt
    36961 ttcaaatgtc aatccgtcct tttcaaaaag agtgatgaaa aatataggga gagtggacac
    37021 aatgagaaat atgactgcta tatcattagg aacgaccttt cgcataaata aatagatagg
    37081 tattccaata agtcctgcaa tcgtaaaacc tatcagttgt cttttcgtca agttaaatgc
    37141 cacctttgtt tttaacttct ttaagtcttt ggggataggt acatacgcca taacttacct
    37201 cccatcttct tttccctttg aaagctcctc gatatgctca tatacggagc cgatttcttc
    37261 ctgaatactt gtaatctgtt catctatact tctgttaaat ctttcctgca ttaggcaaaa
    37321 atcattgtgg cagcgaccga tgtctttttt tctttcttcc aagtcattta ccttgctgtg
    37381 aagtctgatc ctatctatca ccgccataat acctgctcca attactacta taaatactgt
    37441 ttttcttttg ttcatattac ccttcctttc ttttagtgtg catttaatac gcttttggcc
    37501 agtgttccgc tctttaacat cattaaccct agcaaaaccg catatccaag tatcgtaaag
    37561 gtacttgtgt gtatgtctgt tatctttatt gtcttaacta atactgcgta tattccaaga
    37621 caaaccatta aaaagagtcc ttgtaaacct atggcaaaca atcctttgat atagtttgtt
    37681 ccaatctgtc cccactcttt gtttcccatt gtggcaaatg gaatggctga aaccgatgag
    37741 taaatataaa tctcaaacat tcttccataa accacaagca ttatcacaat ggaaataacc
    37801 tgtatagcta cctttatgag agaggtttca aagagtatca tcacaagctc tccaagacct
    37861 ttgtctttta gagaatcaat cattgctaca atctgatttc cggaaacagt tgctgatgtg
    37921 tttataactc ctgctgcttt attaaccata tgctgagcta catcaaaaac tgccattgaa
    37981 aactcaaagg catgagatac taaccatacg gcaatccaca tctttatgat gtatttaaaa
    38041 aaatcaaacg tatctgtatc gtgcatattg ttcttttgca taaccatatt gataagctcg
    38101 atacaaagga ctgccgttat gattagcccc gctatgggga tgataacgga atcgttaatg
    38161 cttttaataa aactgaagac ctgtccgttc caccccatcg gtgtctttcc tacatccgtt
    38221 gcaatcgcac caactttatc gttgatgtca agaaacatag actctaagtt tgcttggata
    38281 ccgcctaata gaaactcttt gaaaaactcc tctattttgt cgaatatccc aaacatttag
    38341 tttctccttt tcttcttatc tgagtacatt tgcaagaagt ggaatcagct ttaatccgat
    38401 aagtactatg ccacctccag ccataagttg cttgattcct tgactttttg cacccggatt
    38461 gtcattacca taaccttcca tcaggttaat aacgccccat gctccaagtc ctgcacctac
    38521 tgccataact aaaatcttta atacatttac cgcatgtgta aaaaattcca tgatttattc
    38581 ctcctcgttt tcttctttct tttcaatctt gttatatgac tttacaacaa agtttttgta
    38641 agtctttccg tctttttcac gcttcttaaa gtaaccaaag atatggatca aatcgccttt
    38701 ttcaaaggct tttactgttt tcgccttttc accataggct gcacaattga tatattcctt
    38761 gcccttccca tactttttta caagcgtgaa gtttgcaact tccacttttt ctccctcttt
    38821 ttcaaatttt gagaaagtcg gttctgccaa caagttggca ttaatattaa tcatttcttg
    38881 cttcattaaa acttctcctt ttcttttaaa aaagcgaccg gacttcatac tttgccaatc
    38941 gcttatactc ttgctatttg attttccttt aagtaggact tcttatcctt atcattctat
    39001 ctccttaaat ttttacataa aaaaggcgat agcttttact ttctatcacc ttgtatttta
    39061 tatattttgt tttaaaacta tagactgaaa tttgttaaga tttacataca ggaatccaaa
    39121 gttccatttt gtagtcttta ctatacatat ctccttcata ataatattca aagtccggct
    39181 ttcctgaatg aacatatcca tgctcgggaa agaacacttc catcgcatac ttccaaccgt
    39241 tatgaataca ctctggaaca ctacctgtta actctacaac cgcatactct gtttctccaa
    39301 tttctaaaat atctagtccc atacttcttg ccttatctac atctgtaaca atatatcctg
    39361 ccatataatt tatcgtgtta ctatctaaaa catcatgatt atcaagactt aatatatcat
    39421 gacatactcc tacaacttct ccggtcccta agtttaacat ttcttcatga gatacctttg
    39481 aaaatagttt ttgccatagg cttgcacata ttgatgaatt gatgttttgt tgatttacac
    39541 ctgcaactgt aaatgctttt tttcttttga atccttatat tcatacttct tcctcctctt
    39601 acacttaatg ctaattgcgc cctggaaact aatttaaacg gttttccaat tctcacctca
    39661 gaaggagtaa atccatgaaa attcttaaaa gcagtcccaa acgaatctga tgactcatat
    39721 ccaaatttga atgcaacatc aataattttt tcatctgtat ctcttaatat aatcgcagct
    39781 tccgttaatc ttctgcttct taaatattct gaaagtgtca tttcagttag aatagaaaac
    39841 aagcgactga acatcgaata agaatatccg gatattctag ttactttctt ttcatcaatt
    39901 tcatcatcaa gaactgtttc aagataatca attgtactgt taaatgactt gataatattc
    39961 attctaattt cctcctttct ttcggttgat tttagtatat aggaagtttt cttttcctac
    40021 cccacagtat tagtacaaat attataggtt tcatacataa aatatcattt atttaaacaa
    40081 cattatacca aatttataca tctacatata gtgataagaa aaatgttaca atcttgtaat
    40141 tacagtatgt ctattcattt tcactttccc tttacgctta atgtagctct ctatatcaaa
    40201 caaattcctc ttgtcatagt cctcaaggag cttgtaattc ttatgctttg taatatcgta
    40261 tttatctgat agaaaaggtc gaacacctct aagctgaaat atacattttc caccgtccat
    40321 tactgttatc tcatcttggc tcattagctc ctttcctgtc ttttgataat tcaatccaaa
    40381 gctcttttga ttgcttcttg tttccgaggt attgtaaagg tctatcgttt cctttccaag
    40441 tgtttcagaa agctctttta atgtagtctt ttccttcccg ccaagaaaca aggtgctatc
    40501 acaattacct acaattgtat ctgcattatc tttgtagatt gcttttaact gagattgtgc
    40561 ttgaagtatt atacttgctg aaatctctct tgaacggatt gttgcaatta acttctcaaa
    40621 cttaggaatt aagccgatat tagcgaactc atcaagtaga cacctcacat ggacaggtaa
    40681 tcgtccgcca tatacatcat ctgccttatc acataagaga ttaaataact gcgaatacat
    40741 aatagacact acaaagttaa aggtatcatc tgtatcagag ataataacaa agagtgccgt
    40801 ttttctatct ccgagtgtat caagctcaag ttcatcttcc cccatcaagt ccctaagttc
    40861 cctaatatca aatggagcaa gccttgcacc acaagagata agaatagatt tcgctgtttt
    40921 tccggcagca agtttgtatt ttttatattg ctttactgca aagtgcgtag gttctttctt
    40981 ttccaatgct tcaaataacc tatcaatagg attcatatat gtttcatcat cttctctaac
    41041 ttctgaagcg tctatcatat caaggagtgt tgcaaagttc ttttcttccc ttggagcttc
    41101 atagaagata tatccaatca gagctgtata atagagtttt tcggctttga cccaaaaatc
    41161 ttcccccgct ttttcaccct ctcccttagt gtttgcaatg attgtctgca ccaatttaag
    41221 tatgtctttt tcactgcgga ggtaggcgaa tggattgtat ttcatagact ttttaaagtt
    41281 aatggtatta aggattttta tctcatatcc gttatcttca agcattttgc cacactcaag
    41341 gactatcgtt ccctttggat ctgttacgca atagctactg tgcatttgca ttaggttcgg
    41401 cttgacataa aaccttgtct tgccacttcc agatcctcct atgacaagta catttttatt
    41461 tcttgcatac ttgggatttt tcggtctacc attcatagtt aatcgttctg tttgcgttag
    41521 caagatattg ttttgaaact tttcatcaat gtacggctca atatcttttc tagttcccca
    41581 tcttgccgag ccatactctt ttccctgcct aaactttttc gcatttttcc cttttgtata
    41641 ggcaatgaat ttgattaaag tagctactcc tacgcctatt agaatatcag ttggatgaaa
    41701 acttggaaga aagctcatgg tgttaagttc caatatacct tgaaatattt tgtctattac
    41761 atcgccacca gtatatgctc ttacatgatg agaaaagata ttgccaacat agaaaaatgc
    41821 aagataggga atgttctgct ttagaaactt tgccttatct tgcaccttaa ataagccttt
    41881 gatgtctttt agtatcttat ctatcatagg ctttgctcct tttgtttatt tttgattttc
    41941 tcttttgaaa tagagttttt agccatctct tcgaacttct caatattctt gtgaatagat
    42001 tctttccttt ctgctttctt ttctgatttt gaaagagcgt gcttaaaagc tttatccata
    42061 actttcatat ctttggcttg aaagaataca gagttttttc ctgtctcttt atttttcatc
    42121 actgaaaatt taacaccgta tctattcagc tcttttttaa gttctttaag ctcagcttct
    42181 tccactggga tttcttcaag ctgtcccttt ttaaccatat cttttagctt tacttcattt
    42241 ccgttgacat tgaccagttt ttcaagtcct ccgaatttct ctgcttcttt catcaatttt
    42301 tttaataagt ttaatagacc ttttccagta actttaccag catttatctc catatttaag
    42361 gtctttcttg ctatttcttc attgatcaca tcaatatcct cctctcatat catagcttac
    42421 aagagccgaa taataggcat ctattccact tggtgcatta taagcggcgg tcagtacaaa
    42481 agctcttata tttctaatct tatattcatt ttctcttaat actcctacta agtactctaa
    42541 atgtcctgaa ttaagtttcc taaatctttc ctttactact tgtacagata tctttgtttg
    42601 gttgatagta ataaaagatt catcatctaa taccattaca tctgccagta tctttaatat
    42661 ttcatcaaac tcttcagcca cactcttatt cccaatgctc atatgttcat agtatcctgt
    42721 ttggtgtcgc aattcttcta aacaattttg atatatattt ttatagctta tatctatcct
    42781 ccctcccttc atattctctg aaaataattg attcttttct ttgatggaag gatggatgga
    42841 aatatatgct gaattatttg tagtattatt tgttgtattc tctggtaatg gttggtgcaa
    42901 attatccttt tgcattagtg cattttgaac ctgtgcatta gtgcaatctg cattattgca
    42961 ttggctgatt ttgcccttat ccattggtgc attttgccct aatgcattgg gcatagaatt
    43021 ttttaatgct tctgtttgta ttttttgttt ttttaaatcc atatccatat ataattcttc
    43081 aagtttatca ttattaattg tataccattt tgttttatct cttggatcct tattgtaatt
    43141 tccaactaaa agataacctg ctttttctaa ctttgaaaaa gttcttttta ctgtatcaaa
    43201 gctcatatag tcaaagtcat tttcttgcca tgctcgaatt gaattatatg tccagtattt
    43261 cccatcatgg taattctttc ccgatttttt atttatttct atccaataat tgatctgctg
    43321 tagtacaagc gcttcgttta atcctaattc tcttgcaaga gttttattcg cgactatagg
    43381 ttgttcatca aataaataca taccatcaaa cactcctttc ttttttcaaa taaaaagacg
    43441 atagtttttc tatcgccttt gataacccat ttatgaaatt tttaacttta ctgtctgtaa
    43501 tttataatac tattttcttc cattttctaa ttttgttcct gaaagtttta aaaggagcta
    43561 cagtattcac atgaataaac ttataaactt cccacactgc tgttttagtc gcatcatcag
    43621 cccattttct catatgtggt ttaaataatt cttcatcact taatgaatca atcattacat
    43681 atatagaatt aatattttca tttaatttag attttaactc ttgcaatgat aaataagcgt
    43741 aggtatctgt gaaccattga tataattcac caagttgatt ccatttgaat tcatccgatg
    43801 gtgtctttac ttgaagccca tttctttcat cttcttccca tttaagaact aaagttgtcc
    43861 atcccacctg ataggcaaga ttttctgccg gcgttctgtc aacttcatca attcttttat
    43921 cttttaagct ttctggaata ttatcaaatt ctgcaatata cttctcaaat gttttattta
    43981 tttcagtttt gagttcttct ttattttcgt atactcgcat aaaattacct ccacaacttc
    44041 tgatttatca attctctgat ttaaactgac attattttct cgtttatacc ttgcaataat
    44101 gctataattg acgctccaac catcggtatc ccataaggac taagtaaaaa tcctataatt
    44161 aaggcttcta taccaatggt aacctctttt tgtaaaaaag atgctattgc tccaaatata
    44221 caaaacatca tcagcaagta cagtattact gttcctatgc caagtaaaaa tgaaagaact
    44281 gcagtgagga tacttaacaa caagctaatt ggaaataaga taatttttaa tatccatctc
    44341 ataaataccc tccatttcta ctaaatcatt ttaaaatgtc ttaaagcatc catgatagct
    44401 tcttcttttt caggcgtaca ctgtgggata gcctgattat cttttttaga tttattataa
    44461 tgttctctca tttcaatccc acatttcttt ttaatttgtg caatataaag tgtagaaact
    44521 tttagtccaa atttttctaa tacatactcc ttgatttgag cgtatgttgc tttactctct
    44581 gcacttgtca aatcaagctc atccagctta atttcaacat ctatatgctt atcgacatca
    44641 agtttggaca ataacgctac cgtctccaca tgtgtagaat gagggaacat atctacaggt
    44701 tgtgcctcaa cacatttaaa tccgtgaaca tttagataat ctaagtccct tgctaaagta
    44761 gaaggattac aagaaacata gacaattctt tccggattca ttgaaacaat agtctctaat
    44821 accttttcgt cacatccctt tctaggtggg tctacaacta ctacattggc agtcttaccc
    44881 tctctataca tcttaggtac aatttcctct gcctttccaa cataaaattc agcattatgt
    44941 atattgttta atttagcatt tatcttagca tcttttatgg catcatctat aatttctatt
    45001 ccataaacct ttttagcctt ttgtgccaaa aataaagata ttgtacctat accacagtaa
    45061 atatcaaaaa cagtatcagt atcatttaaa tttgcatatt caagagcctt atcatacaat
    45121 actttagttt gaacagggtt tacttggaaa aatgataatg gtgaaatctc aaacactaag
    45181 tcacctatat aatcgtttat ttttccatct ccatatataa ctatattttc tcttcccaga
    45241 atagcattcg tatttttttg atttacattt acaaccaaag tcttaaatcc aggtatattt
    45301 tccttaagca ctgaagctaa ttctttaaga tatggaagtt tatttccatt cgctactaat
    45361 acaaccatta cttctttagt cgtaaaacct atttttgtta ctaaatgcct aatcaaccct
    45421 gtgtgagtct tttcatcata tatacttact ttataggccc ttatatatgt ttttattatt
    45481 ttaacaattt tatcatttat ctcatgttgt attacgcatt tttctgtcga tattacatca
    45541 tgagtctttt ttttataaaa gccaataatt ggaataccat tattcttttg tattggaaat
    45601 tgtgctttat ttctatatct atatggatta tccattccta tagtgtcatg aacttctaca
    45661 tcctctaact tacctattct cgaaagtact tgtttaactt catttgtttt catttcaagt
    45721 tgtttattat aatctaaatc ttgtatctga caccctccac aatccctaag attgtcacta
    45781 cacactcttt taaccctgaa tggagacttt tctattatct caacaatatc tcctacagca
    45841 taatttttct ttactttatt aatcctaact ttaactttat cttgtattaa tccgccctct
    45901 acaaaaactg taataccatt gtactttcct actcctactc cgccttgccc aatgtctact
    45961 atatctacaa tatacatctc gtttttcgaa agcataaatc accctccaat caatctatta
    46021 tatttaaatc tttacaaact tttttcttta aatttatatt aaaatattgc tattaattaa
    46081 actatatttt ttactaagct acaatatttt attttaccaa aaaaaaagga gcttataaag
    46141 ccccttagta aataaattta ttatttttct tataaaaccc tagttgaacc tctatatacc
    46201 acacctcttg atgcatccac agttacaact tctccatctt ttactagatt tgttatatca
    46261 gttgcagaca caataactgg tatatctaaa tttataccta caatagctgt atgtgatgtc
    46321 attccaccat tctcagttac tacagctcct gctttttcta tataattgtt catttcagca
    46381 tcagtagcag tagaaacaag tatatctcct tcattaaagt ctacacaagc atctccattc
    46441 tttattatac gaactttacc ttctactgtt tgagtaccta cacctatacc ttgcacgatt
    46501 tcttcgctta ttacatgaac ctttattaaa ttagtagttc cacttactcc tactggtact
    46561 ccagcagtta taactactaa ttctccatta tctatataat cagcttgtct agctgcctca
    46621 attgcctttt ctataacttc atctgtattt cctgcaacag aagacttaac tggatatact
    46681 ccccatgtaa gagctaattt attcatagtt ttttcattat ttgtagtagc tattattgga
    46741 gattttggtc taaatttaga taccattcta gcagtatatc ctgaagaagt agatgttatt
    46801 atagctgatg catttaaatc tactgctgtt gtacaagttg catggcttat tgcatctgtt
    46861 acagtaacat tgttagtacc attttccttt aataatctat cataatctaa agtctcttct
    46921 gttctttttg ctattgtagc catcattttt actgcctcta caggatattt tccagcagca
    46981 gtttctccag ataacattat agcatctgtt ccatcatata tagcattggc aacgtctgta
    47041 acttctgctc ttgttggtct tggatttctt atcatagaat caagcatttg agtggcagtg
    47101 acaactggct ttgctaattc attacatttt tttatcatca ttttttgaac tattggcatt
    47161 tcttcagttg gtatctcaac gcctaagtca cctctagcta ccattatacc atcagaaact
    47221 tttaatattt catctaagtt ctctacacct tcttggtttt ctattttaga tattatttgt
    47281 atatcagttg cattattgtt ctctaatact tctcttattg ctagcacatc agatgccttt
    47341 cttacaaaag aagcagctat ataatctatt ccttgactta tcccaaactc tatatcactt
    47401 atatctttag gagttattgc tggtaagttt atcttaacac caggtacatt tactccttta
    47461 tgatttttaa ctattcctga attttcaaca acacaaatta tatcttctcc atttatttct
    47521 tttactctta agccaactaa accatcatct attagtatag aatctcctga tttaacgtct
    47581 tcagcaagac ctttatagct tactgtacac atttctttag ttcccataat atctttcata
    47641 gttatagtga acttttgccc ttcttctaaa aatacctctg ggtcttcaaa gttacctgtt
    47701 ctaatttctg gacctttagt atctaataat atagcaactg gcttatttaa tttttctcta
    47761 actttctttg ccatatcaat tctttcttta tgttcttcat gcgaaccatg agaaaagtta
    47821 aatctacaca catttagacc attttgcata agttgagtta aaacttcctc actttgtgat
    47881 gctggaccta gtgtacaaac aatttttgtc ttctttacat tatttaacat atttgctcct
    47941 cctgagatat tattccacag atttattttt aatactaaaa cttatataga taatattttt
    48001 gccatctcat aagcttcttt atcaaatact ttcttttgag ctaaagcttc acttatttct
    48061 aaatcaatta ttttgttttc ttttattcct acaactctag ctgattttcc atctaataat
    48121 aattctactg ctcttactcc aagtctactt gctagtattc tatcagatac agttgggctt
    48181 ccgcctcttt gaacatgccc caatacagtt actcttaaat ctgctcctga ctctttttta
    48241 agttcctttt ctaaatcgct agcacttccg actccttctg ctaaaacgat tatactatgt
    48301 ctcttacctc ttttttgagt agtctttaat cttagagcaa cttcatctac tgtaatttct
    48361 acttctggta ctattatagt ttccgctcct cctgcaagac ctgcatataa ggctaaatct
    48421 ccacagtgtc ttcccataac ttctactata ttaactcttt catgagatga tgatgtatct
    48481 cttatcttac ctatagcatc tataatagta ttcattgcag tatcaaatcc tatagtataa
    48541 tctgtatatg ctaaatcgtt atctatagtt cctggtatac ctatagctgg gaatcctaag
    48601 tcactaagtt tttgagctcc tgcaaatgaa ccatctccac caattacaac taagcaatca
    48661 attttatact ttttaagaat ttttactgct tttaatctac cttcttctgt tttaaattct
    48721 tcacatctag aagattttaa tatagttcct cccctgtgta taatatctcc aacagaagat
    48781 aagttcattt cagttaaatc ttcttctagt aatcccttat accctctatt gattccatat
    48841 actttgcatc cataatatat tgctgatctt acaaccgctc taatcgcagc attcattccg
    48901 ggagcatctc ctccacttgt tagtaatcct atagttttca ttttttacct ccttggattt
    48961 tatgtcaatt ttttattata atcattgtta ttgtattcta atggactttt tttgtcccta
    49021 tatcactttt taatcccatc aagactaaaa aaattaaatg tccttaaatg tccattttgt
    49081 gatattatat cacaaaacgg acaatatttt tcatattatt ataatatatt tagattttta
    49141 tataaaaaat caagctttta cttttatgtt atctttagga agtaactctt ctaagttatt
    49201 tataaattca tcacatatac aagttaatac cccggtcatt ttatatgctt tacgtttacc
    49261 atttatattt tcatccatag ggtacaagta tatactgtca ttacctggat attgtttgga
    49321 tatttctaca atattattag ttgtatcggt atcagacata ctatctattt tcaaatacaa
    49381 ttttttacca ctttctggct taatattttc tacatttcta gttcttcctg aattgtagtt
    49441 tctatctgca aagtcagtat catcatttat atcttttatc tctctagcta taagttttgc
    49501 attttcgcct tcttttatac ttaatgttcc ttttacatat attatattat cttcattcaa
    49561 aatgacatta tacttttgta ataattgagg aaaaactatt atttctatag caccatacaa
    49621 atcttctaat tcaataaatg ccataatttc atttcgtttt gttgttttaa tagttttagt
    49681 tacaatcatt ccacccatta taacatctgt ttcattcatt tgtaggtatg actcttcttc
    49741 ttcttttaat gaatttaatt ttccattatc catagatgtt ttttcttgta attctttttc
    49801 aaattgtgat aaaggatgtc cacttacata cattcccaat acttcttttt caaggttaag
    49861 tctctctctt tcttcgtact cttgcctttt aggcaaactg tatatttgtt gaaaatcact
    49921 atcttcctca ctatctccaa aagcatcaaa taaagaaatt tgaccttgaa catttttctt
    49981 tctatccata gatacacttt ctaataaact ttcataacca gccatcaaac tagctctatt
    50041 ttcacttata ttatcaaaag ctccacattt tattagactt tctattactc tcttgtttgt
    50101 atctttttgg tctaatcttt tgactaaatc tactaaatct acaaataatc cattagattc
    50161 tctttcagcg attatattgt ttattatatt tacgccaaca ttttttacag ctgctagacc
    50221 aaatcttata ttgtcacctt caactgaaaa ctttgaaaaa cttttgttta tatctggagg
    50281 taatacatct atttctagtg ctttacattc tcttatatac tcaactacct tatcagtatt
    50341 tcccattaca ctggttatca atgctgccat aaattcaact ggataatatg tttttagata
    50401 agctgtctca tatgctaaaa ccccatatgc agctgcatgt gatttattaa atgcatatcg
    50461 agcgaagtct atcatgtcat caaatatttt attagctatt tcctcttcta caccatttct
    50521 gatacaccct gcaatttcta tattaccatc ttcatcaaat ttaccatgaa taaaatattg
    50581 tctttcttct tccatcacgt ccatcttttt cttactcatg gctcttctaa ctaggtcact
    50641 tcgtccataa ctataaccac ctaaatctct aacaacctgc ataacctgct cttgatatac
    50701 caaacaacca tatgtaactt ccattatagg ctttaatttt tcatgtagat atgttacatc
    50761 ttttgggttg cttttatttt ttatatatgc aggtatagaa tccattggac ctggtctaaa
    50821 tagggatatt cccgctacaa tatcttcaaa attgtctggt tttaattgtt tcataaaact
    50881 tctcatacca gcactctcca actgaaacac ccctaatgta tttccagatg aaagcatttc
    50941 atatactttc gggtcatcat aatccatctt tgaaaagtct attttttctg tgtagttttt
    51001 tatatctcta tttttttcta ttaaatcaag agtatctctt ataactgtaa gagttcttag
    51061 cccaagaaaa tccatcttta aaagcccaag ctcttcaagt gttgtcatag taaactgtgt
    51121 tgtgattgca tcttgatgtt tgtacaaagg cacatactca tctacgggat tttttgatat
    51181 aacaacccct gctgcatgag tagaagcatg tcttagcatt ccctctatct gttttgatat
    51241 atttattatt tcttttgttt ctgggtcttg gtcatacaat tctttaagat taggatttgt
    51301 atctagggcc ttatctattg tcattcctaa agcaaatggt atttccttgg ctaccttgtc
    51361 aactttattg tagcctatat ccaatactct tccaacatct cttattgcag cttttgcacc
    51421 cattgttcca aaagttatta tctgggctac atggtcatca ccatattttc tctttacata
    51481 atctataact tcttctcttc tctcataaca aaaatctata tctatatctg gcatggatat
    51541 acgttctgga tttaaaaaac gttcaaataa tagagagtac tttattgggt ctatgtcagt
    51601 tatcttaagt gtatatgcta ctatcgaacc agctgcacta cctctacctg gacctactat
    51661 aatattattt tctttagaaa aatttataaa atcccaaaca attaaaaaat actctacata
    51721 acccattttt tcaattacat ctagttcata atttaatctc tctagtatct cattgctagg
    51781 attatcatat ctctctttta atccattaaa acacaactcc cttaaataag tttctggtgt
    51841 atacccttct ggaacatcat acttaggaag atgtatagta ttaaaatcaa actccacatt
    51901 acacatatca gctattttca cagtattgtc aatagcctct aatgcatatg gaaataattc
    51961 ttccatttct tctcttgatt ttaaataaaa ctcatcacta ccaaacctca ttctatttgg
    52021 gtcatttaat gtttttccca tttgaataca cataaggaca tcatgtattt ttgaatcttc
    52081 tttatttaca taatgaacat cattagtagc aactagtgga ataccagtct cttttgataa
    52141 ttttactaat ccagaattta cttctttttg ttcaggtaaa ttatggtctt gaatttccag
    52201 aaagaaattt tcttctccaa atatatctct gtagtcaata gcaaattgtt ttgctttttc
    52261 ataatttcta ttcattaaag cacgtgctac atctcctgca aggcatgcag aaagtgctat
    52321 aatccctttg ttatgttttt taagttcttc catgtcaact ctaggcttat agtaaaaacc
    52381 ttctacgtat gaagttgaaa ctatttttat taaattttta tatccttcca tattcttagc
    52441 taacagaact aaatgtcctt gatatttgtc atagtttggg tctttatctc tcaatccacg
    52501 tgcagctgta tagacttcgc accctataat tggctttatt ccttctttta tagctgtttt
    52561 gtagaaatct ataacgccaa acatacaacc atggtcagtt atagctattg agctcatgtt
    52621 tagttcctta gcccttttta taagtttttt caccctcgaa aacccatcaa gtaaactata
    52681 ctcagtatgt acatgaagat gagcaaaatc tttgttcatt aaatcacctc ctgctttata
    52741 attttaattt taccacaaat atatgtccta aaacaaaaac agagactttt atctcaaaat
    52801 aggaattaac ttcaacatat accattgtat gttataaatt aatgcttatt ctgagattgt
    52861 cccctcaatt tattgcgatg attttttttt caaatctgtc gcaatttctt ctatttttct
    52921 aagtctatga ttgactccag attttcctat tggcggttct aacatctctc caagttcttt
    52981 gagagtcata tcctcatact cgattctaat cttagctatt tcttgtaaat tttctggcaa
    53041 actgctaatt cctatagttt cttgtatgag cttaatattt tctacttgtc ttactgctgc
    53101 atttactgtt ttagaaaggt tagctgtctc acaatttact atcctgttta cattatttct
    53161 catttctttt actattttag tattttgaag acttaaaaga gcgtggtgtc ctcctataat
    53221 actcaacaaa tctgatattt gttcactctc tttgatataa acaacgtgat tattttttct
    53281 agccacaatt ttactattaa aacctaaaga gtttataagt tcttttagag aatctgcaaa
    53341 ctcctcatta ttagttacaa attccatatg atagttcttt tctgggtcgc ttatggaacc
    53401 tccaccaaga aaagctcctc ttatgaaagc tctcttacat ccttcatgct taattaagtt
    53461 ctctgaaatt ttgtttatag taaaaaatcc ttctttattt tcaagtatac ctaacttttt
    53521 taataatatt tctgaaccca tttcgcttgt taccattaat acataactgt tatttctctt
    53581 tagcatttga ttcttattta ctgagattgt agtattgata ttaaaattct tttttaataa
    53641 tttaaatact ttacgggcta ttgaatttaa ctctgttgtt atctttagat ttaacttctt
    53701 atatccaaca atttgtatac ttccccctga tttaactaga gctgacaatt cagctatatt
    53761 acaacattca ttcccagata taactcttgc tagttcattt ttggtttcag ttgaaaaaga
    53821 catatctatc acctattaac taatttttct ttaacctttt ttttatttct tgtaaggcaa
    53881 cctttattat ctccttttca tcatcatacc tagctaggtc taccactcta tctagatgga
    53941 taaatttagc atcaataaag ccttcctctt tttgtggaat agtatccata ttctttgctt
    54001 gcatcaaata ccaaaaaact gttttatgaa ctgctctatt ttcatcccag ttttctttga
    54061 atgtatagtg tatttcacct aaatatttca atatatctgc ttttactcca gtttcttcac
    54121 ttacttcacg taatgctgcc tcttggttat tttccccttc ttctactttt cccttgggta
    54181 acacccaatc tccattgaac ttccttagta aaagaatcgt attgccaaac aggactacac
    54241 caccagcact aacctcttct ctcatcgatg ttcactcctt aaaactaaca taatgataag
    54301 gatttctaca caatttttta aaatcctttt ttactaactt ttgttgtaat tatgatttag
    54361 ggctaactca ataactatgt tagatatata ctttgcatca tgtcttatat aattattttt
    54421 tatttcaatt aaattcttct ctacggtttt tatacccatc tcttttaatt tatctttttg
    54481 ctttttatct aacaagactt gctctgctcc atcttgttta tataagtcaa gcatttcttc
    54541 tggcaatatc tcattatttg cgattacata atctattaaa ttctcatcag tgtgctttac
    54601 tatagcatta acatgctcta atacattgta cccttctgtc tcacctggtt gagtcattat
    54661 atttgctata tatacttttg gagctacaga cgatttaata gcgtctacaa tcccctctac
    54721 aagtaaattt ggtattatac tcgtatataa gcttccaggg cccataatta taatgtcagc
    54781 atcgtaaata gatgcaataa catcctttaa tggtttgaca tcttttggat ttaaaaaaat
    54841 tttatcaata gagctttttt gaatttttga ctcttcaggt atactagatt ctccatttat
    54901 tatattgcca ttttctaatt tggcaactaa atttacatct tccaaagtta ctggtaaaac
    54961 tcttcctgtt atagcaaaaa tttcacttaa tttatataca gctttttcaa agtttccata
    55021 caacccattc atagcagcca aaaacaaatt tccaaaactc tgacctttta aaagcccttc
    55081 tgtaaaccta tattgcataa cttcattcat tgttggctct atatttgcaa gtgctaatag
    55141 acagtttctt atgtctcctg gtgggagcat tcctaagtct tctctcaata cacctgaacc
    55201 tccaccatca tcagcaacag ttacaattgc agttatattt tgcgtagtgt gtttaagacc
    55261 tcttaaaaat actgattgac cagtacctcc acctatcaca accacatttg gttctctatt
    55321 ttcaacaata gacctttgaa atagttttac tttttcattt tttgcttttt tatatataaa
    55381 taacgataca attaatgcaa tccaacctaa aattcctaca ataataagta atttagccat
    55441 gtttgcctcc attatcgcaa catagagtct ctatgtttct ttacaactct atatccctta
    55501 ttagataaat cctccgctat aagatttgtt atagtaactg acctatgtct tccacctgtg
    55561 catccaacac caattactag atgttgtttt ccttcatcta tatattgagg tactaaaaaa
    55621 tgaatcatat ctaaaagttt cacataaaat tcttcactga ttttagaatt cataacataa
    55681 tctctaactt ctttatcatc accagtttta gctcttaact cttcaacata atagggattt
    55741 ggaagaaatc ttacatcgaa tactaaatct gcatcagcaa gtataccatg tttaaatcca
    55801 aaagatacta cagaaatagt taggtttgga ttatcttcac cagaagaata tattttcttt
    55861 atttcttcct taaggtcttt aggtttcatg ttagttgtat caattataca tgtagataac
    55921 tcctttaaag gttccattat ctttctttcc atttttatac cttctggaat ctgcatatct
    55981 tttgccaatg gatgatttct tcttgtcatt ttatatctct ttaacaatac atcatcatta
    56041 cagtccaaat ataccatttc atattcataa ttttcttttt ctaaataatt taagctttca
    56101 tgaagcgctt caaaaaactt tctccctctt atatctatcc ctagagcaac tttatcaata
    56161 cttgaatttg gttgataaca taattctgca aatttagtta tcaaagcagg aggtagatta
    56221 tctacacaat aaaatcccat atcctctaaa gctctcattg tctcactttt tccagatcct
    56281 gaaagacctg taactataac aaatttcatt gctaactcct cccttttatc ttatgtttat
    56341 tatattataa tgttttctac taacgatata tccagattag agtcagatat agtcttcata
    56401 gctagtttaa aatcaccaac tctttctgga caatgtgcat ctgagcctat tataaattta
    56461 ttttctagcc cttttatttt atttaattga tttacattca aaaaagaatg gctactattt
    56521 atttcaagtc tagtatttgt cttttttgct gtctttgcta cttcttctat atacacatcg
    56581 cctttatctc ctggatgagt aattataaaa atatcattat tacttatcgc atttattaca
    56641 gcttttgtat tgtattcttt tgctttttca gtttttaaaa tatagttatt acaatgattt
    56701 aataagcttc ttaatgatgt tggagtagaa ccaaaatgat atccagccat aatataatca
    56761 aaatatctaa taaatttatc ctccatatct atattgcctt tatcatctaa tatgttacac
    56821 tccataccta aaagtatatt tatatcattg tacttatcat ttaaataatc tacttcttct
    56881 ctcattttga gaatatcttt tttctttaca ccatatgcaa tatgtttata actatggtca
    56941 gatattccta ttgtttttat tcctttagaa atggctacct ttacattatc ttcaatagtc
    57001 cctttgccat gactatataa tgtatgtgta tgataatcag ccagtatttt catttgttaa
    57061 tcctctccaa gtattttgac ttcttcttcc aacataaccc caaacttggc atatacagca
    57121 ctttttataa catatattaa atccaaaata tctttagcac ttgcttctcc ttgattgact
    57181 acaaatccac aatgcttctc agatacttgt gctcctctta aagtaagacc tctcaatcca
    57241 gtttcttcta ttaatttacc tgcaaaatat cctgttggtc tcttaaatgt acttcctgca
    57301 ctcgcaaaat ttaatggctg tttagttatt cttcttgtcg ctaaatcatc cattaaactt
    57361 tttatttcat cataatttcc ttcttgtaat tctagttctg ctgataaagc tatatatccg
    57421 tttttcgata ttatactttt tctataccca aattccatct gttcatttga taattcaaat
    57481 atattgcctt ccatatccat cagtcttact gacttcacta tgtccttcat ttcaccgcca
    57541 tatgctccag cattcatggc tagtgctcct cccaatgtgc caggtattcc tgatgcaaac
    57601 tcaaaccctt ttaattcttg cttttggaga gctttaccta aaactgataa taaagctcct
    57661 gactgagcag tcatcctagt atcatttatc tcataagaat tgaaattatc agctaattct
    57721 attaccactc ctcttattcc accatcttta attaaaatgt ttgaaccatt tccaattatt
    57781 aaatacggaa tagattcttc ttttaccaac ttaagtacat tttttaattg ttcttcagtt
    57841 ctaggtctaa ctaatatatc tgctggtcct cctaccctaa atgaaatatg ttttttcata
    57901 ggttcatcga cttttatatc ctctttactt aatatattta ataaatttat atatatatct
    57961 tgattattca ttgtatttcc tcccaaatta tactgaactt tagctgtcat tattatttaa
    58021 attacattag tatatagtat taactatatt caataatata tgattttgct tatcatatgg
    58081 attttatatt ttaagcttct taattacaaa ctatattcaa taaatatata ataaagtaaa
    58141 aaaaaaaaaa taacaacata taaaagaata taaataattt aaaaaaagca ttaatatcac
    58201 tgatatcaat gctttaaaag gcaatttaat tcgtattatt tatctaacta tttttgacat
    58261 agcaaagata ataaatccct ccaactacta tggaacctcc aacgatattt ccaagcgtta
    58321 caaataccaa attagttata aattgaacca tagttatatt tgctcctaat gccattccca
    58381 ttggtataaa aaacatattt gcaacactat gttggaatcc gcataataca aataacatta
    58441 ttactgtcca acatgaagcc aattttgaca ttatatcttg agatgctgta gccatccata
    58501 cagctaaaac gactaaaata ttacaaagta ttgcttttaa aaacattgat gctatagttg
    58561 aattaacctt agcttctgcc acagctatag cttttgtagc tgcatctcct gtcaatgtag
    58621 aagaataaaa cacaagtgca actagcagaa ttgaacctac taaatttgca aaataaacca
    58681 gtccccagtt tcttaacatt tttcctaatg ttattttttt gtccataagt gcaatagcca
    58741 ttaaggtgtt tccagtgaat aattctgctc ctgccaccac tacaagcata agacctactg
    58801 gaaagaccat agcacttaaa aagctagcta cccctgggtc tatcttgcca aaagtttgac
    58861 taactatcat actccccaat gccccaaaac ctataaatgc tcctccaaat aaacctaata
    58921 agattgcatt ttgtgtcgaa atgtttgctt ttttaatgcc tgcacttata gttgcttgtg
    58981 cagtttcccc aggtgtcaag aatcctttac tcataaatcc ttccccctta aaaattatac
    59041 tttcccttgt ctttttatgg caataattat ataataacaa tatgatatat aataaatttt
    59101 att
//
LOCUS       DAEMAE010000005        53246 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEMAE010000005 DAEMAE010000000
VERSION     DAEMAE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.005"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1..633)
                     /locus_tag="KPH93_000238"
                     /pseudo
     CDS             complement(1..633)
                     /locus_tag="KPH93_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            complement(894..1151)
                     /locus_tag="KPH93_000239"
     CDS             complement(894..1151)
                     /locus_tag="KPH93_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG1687081.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(1155..1445)
                     /locus_tag="KPH93_000240"
     CDS             complement(1155..1445)
                     /locus_tag="KPH93_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687082.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            complement(1476..2042)
                     /locus_tag="KPH93_000241"
     CDS             complement(1476..2042)
                     /locus_tag="KPH93_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687083.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSVRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            complement(2073..2243)
                     /locus_tag="KPH93_000242"
     CDS             complement(2073..2243)
                     /locus_tag="KPH93_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687084.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            complement(2255..2602)
                     /locus_tag="KPH93_000243"
     CDS             complement(2255..2602)
                     /locus_tag="KPH93_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687085.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            complement(3025..4335)
                     /locus_tag="KPH93_000244"
                     /pseudo
     CDS             complement(3025..4335)
                     /locus_tag="KPH93_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4352..5344)
                     /locus_tag="KPH93_000245"
     CDS             complement(4352..5344)
                     /locus_tag="KPH93_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG1687086.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTHRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            complement(5356..5973)
                     /locus_tag="KPH93_000246"
     CDS             complement(5356..5973)
                     /locus_tag="KPH93_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG1687087.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(5966..7012)
                     /locus_tag="KPH93_000247"
     CDS             complement(5966..7012)
                     /locus_tag="KPH93_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG1687088.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLSATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETTELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSTDLVNLVQEEIDPNMKGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWENI
                     KSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINEVKSNLT
                     INSNSIVVRGEVVG"
     gene            complement(7013..7432)
                     /locus_tag="KPH93_000248"
     CDS             complement(7013..7432)
                     /locus_tag="KPH93_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG1687089.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            complement(7437..7697)
                     /locus_tag="KPH93_000249"
     CDS             complement(7437..7697)
                     /locus_tag="KPH93_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG1687090.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPIQIAFGTIESVNPLVVKIEQKA
                     SFEESFLIQTDTFKKYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            complement(7711..9675)
                     /locus_tag="KPH93_000250"
     CDS             complement(7711..9675)
                     /locus_tag="KPH93_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1687091.1"
                     /translation="MDDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQMFETVDKNMNEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGNIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFSSVDEGIKKGISNLSRNYIHTG
                     RKTLERIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSTYTSNNAGTGVGSNEEAEKNL
                     KDTTYQVQNNNSNTLTNNNSKVSKVIQEAKNQLGKPYAWGGNGPKSFDCSGLMVWAFK
                     RGAGINLPRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYIH
                     APQTGDVVKISSLSGRQKKKHDFERARRFF"
     gene            complement(9668..10369)
                     /locus_tag="KPH93_000251"
     CDS             complement(9668..10369)
                     /locus_tag="KPH93_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG1687092.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVLATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKK
                     VPKYTIYKGQVLKLG"
     gene            complement(10385..12751)
                     /locus_tag="KPH93_000252"
     CDS             complement(10385..12751)
                     /locus_tag="KPH93_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG1687093.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADEQQKRFNDDIRKGASSTDKLVENAKRLVA
                     TYIGLRSVGGLINLSDQMTSTNARLVMINDGQQSDGGLNKMIFQSAERARASYLDTAK
                     IVSRVGMNAGKAFSSTKEIVAFAEQLNKKFVIAGATTEETNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLEVDIGKIRGMASEGMLTADIVKNSLLSAAEQTNAEFE
                     KMPYTFGQIFTSIKNNAVMIFGVIQKKIEQSMSSKGFRTFIDNFIDSLYVLGAVAFSI
                     FNGIINILGSPFFQAFVNAIIVGVSLIVQVLGWVITQALNITNVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKTLAMNFAHIIAESYRAAALVASTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAVINKFAGTSYSAIGIVAGALSGLTAFIINSV
                     FFWINVFISFAEFFTNVLDHPVYSVKKLFFNLATAVLNNLISMTKGCDEFATNLANSI
                     IDGINGALKAWNKFVDVLNKFGGLADKLGLGKADMVGHTKSITSTLQKAKGDLNKWLG
                     AEPEGYKSFKQLEYKNVGDWAKNGYAFGQNLENKLKDAFDINKIAEKAKKDLGLDDLW
                     DKKYGLGDGLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEV
                     INKYTGVNIKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGVV"
     gene            complement(12827..13387)
                     /locus_tag="KPH93_000253"
     CDS             complement(12827..13387)
                     /locus_tag="KPH93_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1687094.1"
                     /translation="MFCSNCGAEITGVGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMKISGLNIKEAKKIVDSSFEELKINFIDDTMSNS
                     EKEKIINTQNRKNNIEKAQQESVACCPKCGSTSLSAQKKGFGIGKAVAGATIAGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            complement(13818..13994)
                     /locus_tag="KPH93_000254"
     CDS             complement(13818..13994)
                     /locus_tag="KPH93_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687095.1"
                     /translation="MYENLLKEYNLKTDEDVEYFVKFATLLHKLKQDNEEKFQEYAEI
                     LRGILREQEERKNK"
     gene            complement(14043..14870)
                     /locus_tag="KPH93_000255"
     CDS             complement(14043..14870)
                     /locus_tag="KPH93_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG1687096.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCKIE
                     NKNGKEYKSVRWERMNEFCIEFGFDHKWAKDDYIPESLFYMLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPKKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIKL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRTWIEENNRPAKIEGEKKNFHVVYKIE"
     gene            complement(14994..15167)
                     /locus_tag="KPH93_000256"
     CDS             complement(14994..15167)
                     /locus_tag="KPH93_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBG1687097.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDR"
     gene            complement(15471..16046)
                     /locus_tag="KPH93_000257"
     CDS             complement(15471..16046)
                     /locus_tag="KPH93_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG1687098.1"
                     /translation="MNAVELCRDNFGNFVKNTLIQIVYLGGHPLFNREEMICLLIRSD
                     KIVVKAFGTGPVPIVDVFEVSYSDIKSVSIEKEEEVIRRYTATRIALFGPFALAMQKA
                     ELNKKEYLIIECNDFILSFDKNDEALATVYKNLIEYRKNNGIGNVSESEVEKNKITNP
                     LEQIKTLKELLDMDAITQEEFDVKKKELLNL"
     repeat_region   16341..16635
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16476..16504
                     /rpt_unit_seq="atttacattccatatagttaatctaaaac"
     gene            complement(17371..17532)
                     /locus_tag="KPH93_000258"
     CDS             complement(17371..17532)
                     /locus_tag="KPH93_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687099.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(17873..18313)
                     /locus_tag="KPH93_000259"
     CDS             complement(17873..18313)
                     /locus_tag="KPH93_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1687100.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(18385..18855)
                     /locus_tag="KPH93_000260"
     CDS             complement(18385..18855)
                     /locus_tag="KPH93_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG1687101.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(18872..20182)
                     /locus_tag="KPH93_000261"
     CDS             complement(18872..20182)
                     /locus_tag="KPH93_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG1687102.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(20183..20359)
                     /locus_tag="KPH93_000262"
     CDS             complement(20183..20359)
                     /locus_tag="KPH93_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687103.1"
                     /translation="MSKTLSKEENYKFTKEQIVNSKRYVNRKDLLNAILKEDELYSFS
                     EVEDMINNFMKGVS"
     gene            complement(20352..20789)
                     /locus_tag="KPH93_000263"
     CDS             complement(20352..20789)
                     /locus_tag="KPH93_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687104.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNNTDKMDTVELFGGLKRGDKLE"
     gene            complement(20782..21207)
                     /locus_tag="KPH93_000264"
     CDS             complement(20782..21207)
                     /locus_tag="KPH93_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG1687105.1"
                     /translation="MARWGSVDFREFKRVCKKVEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(21207..21554)
                     /locus_tag="KPH93_000265"
     CDS             complement(21207..21554)
                     /locus_tag="KPH93_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687106.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(21548..21949)
                     /locus_tag="KPH93_000266"
     CDS             complement(21548..21949)
                     /locus_tag="KPH93_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687107.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(21959..22192)
                     /locus_tag="KPH93_000267"
     CDS             complement(21959..22192)
                     /locus_tag="KPH93_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687108.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEGNKQLKEKNKLTKK"
     gene            complement(22192..23091)
                     /locus_tag="KPH93_000268"
     CDS             complement(22192..23091)
                     /locus_tag="KPH93_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG1687109.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(23103..23666)
                     /locus_tag="KPH93_000269"
     CDS             complement(23103..23666)
                     /locus_tag="KPH93_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG1687110.1"
                     /translation="MDWLEELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(23976..24218)
                     /locus_tag="KPH93_000270"
     CDS             complement(23976..24218)
                     /locus_tag="KPH93_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687111.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            complement(24184..25677)
                     /locus_tag="KPH93_000271"
     CDS             complement(24184..25677)
                     /locus_tag="KPH93_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG1687112.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIERDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALVNELHTKLTQSIIRGENPKKLV
                     NDFAKRFKVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            complement(25667..27106)
                     /locus_tag="KPH93_000272"
     CDS             complement(25667..27106)
                     /locus_tag="KPH93_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1687113.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(27112..28365)
                     /locus_tag="KPH93_000273"
     CDS             complement(27112..28365)
                     /locus_tag="KPH93_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG1687114.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(28343..29035)
                     /locus_tag="KPH93_000274"
     CDS             complement(28343..29035)
                     /locus_tag="KPH93_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687115.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(29106..29615)
                     /locus_tag="KPH93_000275"
     CDS             complement(29106..29615)
                     /locus_tag="KPH93_000275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687116.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(29636..30610)
                     /locus_tag="KPH93_000276"
     CDS             complement(29636..30610)
                     /locus_tag="KPH93_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687117.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(30913..31149)
                     /locus_tag="KPH93_000277"
     CDS             complement(30913..31149)
                     /locus_tag="KPH93_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687118.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(31634..32113)
                     /locus_tag="KPH93_000278"
     CDS             complement(31634..32113)
                     /locus_tag="KPH93_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBG1687119.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(32106..32243)
                     /locus_tag="KPH93_000279"
     CDS             complement(32106..32243)
                     /locus_tag="KPH93_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687120.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(32267..33637)
                     /locus_tag="KPH93_000280"
     CDS             complement(32267..33637)
                     /locus_tag="KPH93_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1687121.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(33586..33912)
                     /locus_tag="KPH93_000281"
     CDS             complement(33586..33912)
                     /locus_tag="KPH93_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBG1687122.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(34194..36611)
                     /locus_tag="KPH93_000282"
     CDS             complement(34194..36611)
                     /locus_tag="KPH93_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687123.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(36623..38581)
                     /locus_tag="KPH93_000283"
     CDS             complement(36623..38581)
                     /locus_tag="KPH93_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBG1687124.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(38581..39153)
                     /locus_tag="KPH93_000284"
     CDS             complement(38581..39153)
                     /locus_tag="KPH93_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBG1687125.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(39176..39748)
                     /locus_tag="KPH93_000285"
     CDS             complement(39176..39748)
                     /locus_tag="KPH93_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBG1687126.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(39801..40136)
                     /locus_tag="KPH93_000286"
     CDS             complement(39801..40136)
                     /locus_tag="KPH93_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687127.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(40151..41317)
                     /locus_tag="KPH93_000287"
     CDS             complement(40151..41317)
                     /locus_tag="KPH93_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBG1687128.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(41329..41766)
                     /locus_tag="KPH93_000288"
     CDS             complement(41329..41766)
                     /locus_tag="KPH93_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687129.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(41837..41986)
                     /locus_tag="KPH93_000289"
     CDS             complement(41837..41986)
                     /locus_tag="KPH93_000289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687130.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADVVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(41967..42359)
                     /locus_tag="KPH93_000290"
     CDS             complement(41967..42359)
                     /locus_tag="KPH93_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687131.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(42390..42587)
                     /locus_tag="KPH93_000291"
     CDS             complement(42390..42587)
                     /locus_tag="KPH93_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687132.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            42663..43037
                     /locus_tag="KPH93_000292"
     CDS             42663..43037
                     /locus_tag="KPH93_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687133.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(43047..43256)
                     /locus_tag="KPH93_000293"
     CDS             complement(43047..43256)
                     /locus_tag="KPH93_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687134.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(43303..44133)
                     /locus_tag="KPH93_000294"
     CDS             complement(43303..44133)
                     /locus_tag="KPH93_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG1687135.1"
                     /translation="MSNNLMVFEGKEVEVFEFEGQILFNPKHVAECLDIKNVNENLRN
                     MNNKQVIKLINSKISSTDFRKLHNTGENFLTESGVYKLIFKSRKEEAERFQDWVTDEV
                     LPSIRKTGTYNMVNRQLKDSYMIDNPIERAKRWIEEQKEKEQLQLESKMKEKVIKELK
                     PKADYTDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGK
                     GYTHSETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG
                     "
     gene            complement(44253..45026)
                     /locus_tag="KPH93_000295"
     CDS             complement(44253..45026)
                     /locus_tag="KPH93_000295"
                     /inference="COORDINATES: protein motif:HMM:NF015340.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBG1687136.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKRKKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFDEMEKALKNEQTKLPTTYKEALQQLLIEVEEKEQLQLENQTMKPKADYFDALVER
                     NLLTNIRDTSKELGVKEKVFVLWLIEKKYCYRDLKGKIKPYSNKMQYFELKEFTTPYG
                     HSDTQTLINPKGREAFRLLLIKDGLIREKERECQITLLG"
     gene            complement(45057..45272)
                     /locus_tag="KPH93_000296"
     CDS             complement(45057..45272)
                     /locus_tag="KPH93_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687137.1"
                     /translation="MYVNRLKGLMKENQHTQKFVADLLGLSLFGFRLKLNGKNEFKAN
                     EIKKLSELYGVSTDYFFSELVAKMAIK"
     gene            45453..45998
                     /locus_tag="KPH93_000297"
     CDS             45453..45998
                     /locus_tag="KPH93_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1687138.1"
                     /translation="MFRLKELREEKGISLDRLSMDLNVNKSTLSRIENGLREPKQSFI
                     EDCSNYFDVSTDYLLGKTDIKNPNQVVKSLNPIFSKRLRELIKEKGVTLNILGKKLEI
                     STDLLSKYENNIISPKSDDIIKLAHYFDVTTDYLLGTTLVRNHIDTVAAHKANPHEDL
                     PEEAQEQLNDYIEFLMNKYKK"
     gene            46022..47611
                     /locus_tag="KPH93_000298"
     CDS             46022..47611
                     /locus_tag="KPH93_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG1687139.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            47874..48380
                     /locus_tag="KPH93_000299"
     CDS             47874..48380
                     /locus_tag="KPH93_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG1687140.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            48620..49750
                     /locus_tag="KPH93_000300"
     CDS             48620..49750
                     /locus_tag="KPH93_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1687141.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINRKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(50108..51796)
                     /gene="ilvB"
                     /locus_tag="KPH93_000301"
     CDS             complement(50108..51796)
                     /gene="ilvB"
                     /locus_tag="KPH93_000301"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG1687142.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMLVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFGLDDETNTKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLLEMVESKNNSNWKEEIKTFRKSEGIQTDEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLFNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKTIDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(51849..52844)
                     /gene="ilvC"
                     /locus_tag="KPH93_000302"
     CDS             complement(51849..52844)
                     /gene="ilvC"
                     /locus_tag="KPH93_000302"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG1687143.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMLLMPDEKQKKVYEESVKDNLKE
                     GQTLVFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKIREEEYNTEIAEVGRSLRSMMSFLK"
BASE COUNT        16334 a         9864 c         5705 g        21343 t
ORIGIN      
        1 atattctacc ataatgagcg cattgatttc tgacctgtgt taatgattgt aaccaagatt
       61 taactaataa agtattcact ctacacaaat tattttttat gtatctagta tcttctggca
      121 acatgtttga atataatttt gacaacatac caaaagtcat tatttctgtt gcaacccaaa
      181 tgggaagttt tccttcatat ttttctttat ggtgtataat aaaaagctta tctgaattat
      241 tatttttttc tctctctaat gctgtcaaaa aattaatata gaatttttca tctttgaaac
      301 tctcccttta tagatatcca caagcaccat gtttaattgc taatgtatat ccaatgtatg
      361 tcctaaatgc tatttctatg ctccctaaca aatctgttaa taatatccta aattccttat
      421 taaacctata tatatcataa acttcttcaa acgtagttcc ttctttatat gagccatcat
      481 cattcttaaa acttagaaga tatgctgtaa acctataata attgacatta cttaatacaa
      541 atttagcatc ttctttatca tttattatta atcctctact ttttaaaata tctatttgtt
      601 catcaaatgt ttttcttctt ttacttcaac cattgttttc accttttcat agatatactt
      661 ttatataata aaacccacca attaatatta gtgggtaaat ttatctgtcc cgactatttg
      721 agctataaaa gaagcttggc gggttccttc aaaaatcaat gtcccgtata tttgagcata
      781 taaatgcttt acgagttctg tactattatt atatacttat tagctataaa agtatactct
      841 atttaatgaa attttttaaa tttttgtgag taataatatt tttgttaatt tcattacttt
      901 tcaccatcct tcaactgttt gtaagtttga tttataccta ttgcaactcc ccaacaaatt
      961 acaccttgta agactgcaac aggatttaat cctagcatcc atattgagaa acctattcca
     1021 agtatcagta acactactgg aatgtattta ttatctaatt gtttatattt cttgaatcca
     1081 acacctaaaa cataaagagc agctactaaa attagcaact gctctggtat aaaacttatt
     1141 aaattgtcca tcttttattt tcctccaatt ctatttataa tttcatcaat tctaagatgt
     1201 gcttgttttg tacttgcttc tacttttgca actcgttcac tgatatctaa gaaacttgta
     1261 ttaaactttg ctacatcatg ctttatatct cttacattct cacacagaaa agttatttgt
     1321 tgttctaatt ttgtagttgt ttctgtatca tcttgtatct ttctgtttga gctatttcta
     1381 aatgctaagt aggatattac aactcctgca actgcacata atagatttat gctaatttct
     1441 tccatacatc ctcctagttt taataatata atttattatg cgaaacaagc aatataataa
     1501 cctgtaaaag gatatggctc ataactagta tcatatgaat gaactgaaaa accagtttta
     1561 ttgctgtcta tagtaatcca tgcacgttca gaacctcctg tattacttcc taaagaacta
     1621 ccactgggat tataataata tacacttctt ctaaagtaaa catagacaat aaggtaactt
     1681 gcactcttgc caaaattagc agaaaaagga ttaccatcat atccatcaat aggaccatat
     1741 ctaacagaat atttgctttt caattgagtt tctaaattgc ttatattgct cttaaatcct
     1801 gtaatatagt tcgcataatc ttgaaatgtt tgttctgatg tagcaggaga gccgatagca
     1861 gttgataaaa gtgtttttcc actatcggct tcttgaaaag cctcatctgc tctatctata
     1921 cattctttca atgctccttc tacattttca cttgtaaatt tactttctgt atcttctata
     1981 gttacattct ttgcttctaa tacaagattt ctaactttat taactaactc tttaaaagtc
     2041 atttagtcac cttctttcaa taaaaaaaga acctatttta ttggttctgc tggtgtttct
     2101 tctttattta ataaacttgt aagttctaaa tattgttctt ctgtaattct atttactgca
     2161 tagaatacat caattttgtg ttgcaaatcc tctttagtgc tatagttctt ttgttctatc
     2221 ataagtttta ataagttata catgttaatt cctcctataa attgttattt aattttatat
     2281 tttctacctc aaaggctgtg tttactatct cactatctct atttttattt tcttctttta
     2341 acatgcttaa ttctttttct aatgcttgta atctcttttg ttcatctgtt aaaatgactt
     2401 ctatgtcttt gatgattggt tcttttgtaa ctggatttat agactctata tattgtttac
     2461 tatagtctat actaccaaat tcaacatcca aaaaatttaa ttcagttatt tttgaccact
     2521 cttgtatatc tcctgttgct tcaccagttt gaagccatat attgcctgtt tggtcgtaaa
     2581 ttattctatt atttctgttc atattatcac ctcatttttt tattaaatat atattttgta
     2641 agttaatgta gctccctctt ttgcccatat tccaactgct tctgacattg ataatttata
     2701 taatgtaagc actaatgaag aatacccaac attagataca ttaagaatac caacagcttt
     2761 cgggttacta tgagtagaat cagaattata gccaaaatta taaaaatttg aattacaaac
     2821 attatcaaaa taaacataag atttacttgt tgtactaaaa gttactccac taagtacaat
     2881 taaactagga gagaagccca tgttaactgg tatagtaaga gtttttgaag ttccttcttt
     2941 gtcatatata cttaagttaa aattctctgc atccgttttt gtgaatgtat aagttcctgt
     3001 tataaatctt tttcttttac ttaactctgt ttctaattga gttatagtat tgtttttttg
     3061 tgttacttga ttttgtaaat cttgcacact agtgtctgaa ctatcaaaac ttgtttttat
     3121 tttctctgat aactccacaa gtgtattatt taaacttgct tctatatttt taagtgctaa
     3181 agtgtttata atacttgttt tcccaacttt aaatcctgca ttaacctcaa ctaattttgt
     3241 tgatatatca tttaaattta cattttcggg cagtggcatt atattcttac ttatacttaa
     3301 cactttttct gctgtagcat tattactgtc tgtaacaact attttaagtg tgtgtaatgc
     3361 attatcttct aatgtataat taattgtttt ctcttctgtt aaatttgttg ttatagtttc
     3421 ttttagtaca tcatctataa accattctat ctttgtaagt aatgcagggt ctgtgtggtc
     3481 ggctttaaat gttgcctgtg tagaattata agaagatact gttaaaaatg gcaatgcttg
     3541 tagtaatgtt attttagcat aaccatctgc tttagtagta ttacctccag tagtcatgac
     3601 tatattttca agataatatt caggtgttgg tatatatccg ggtgccttat aactatcttt
     3661 atttagtgcg taaccacttc caccacctcc acattcatta gaataagaac cagcaccacc
     3721 gtaccaaccc cctccaccac ctattcctat gttaccataa cctccttttc ctaatgaacc
     3781 atggtattct tctgtgtcgt aacttgttcc accttggtat tgagaaccac cgccacaaaa
     3841 atctctgtca cgaccaactc cattaacacc tacataacca ccaccatgac caatagaacg
     3901 agcagaagca aaattatttt tcatacctcc tccaccgcct gcaacaagta tgcgtgaaag
     3961 caaactttca gtgttacccc aagttgcact aggatgataa agtcttatat cagttgctcc
     4021 accaccgtat ttagaataag caaagctacc agtagtaact ttgccagcag cgcctgcacc
     4081 gttaaaacca cttctagtaa ggcttgaacc ttcagaaact ttctcataac cggattgacc
     4141 gacacaaatt tgtagattag ttcttttttt aaatacaatc tcaccttttg aataaccacc
     4201 ttttgcacaa tcagtccaat cgcttgtatc gacagcacca ccacaagcac cccaacattc
     4261 taatttatat cgcccaggtg gcaatgaaac attttgtaca taattagcat aattaaaatt
     4321 ccattcagtc tgcattttct cactctcctc tctaacaata agttatcaac tcatttacac
     4381 ttgttgcaat attagataaa ccaccattta ccttttcttc tatattaacc aatctgtcct
     4441 ctattttctt agatgaataa gtagtcattt cagatactct gttatcatct acagttgcat
     4501 ttataaaatg agtttctgca tttccattta tcacatagac atttaactca acttttactt
     4561 cacttctaat ctcaattgaa ttatcatcaa ctattttaaa atttggaact atattttctt
     4621 ttgtagtagc atctataata tttacaacta ttctatgtgt taataaacta tgtgttacag
     4681 ttgctttgaa tccactttct gcatcctcaa cccaatcatc aattgcgacc ctttgagtgt
     4741 atgcaacatt tgagccacct gcgattaatt ggtcgatttt agtatttaac tctgttttag
     4801 cagtttctat gttgcttgtt aattctgttt tagttgtatc aattttagta ttaacagtac
     4861 ctattttagt ttctaagtct tgtatatctt tgagtgttgc aaagattatt gttgggtcaa
     4921 ttttaagttc tatattattt acattagata caataagcac agttttaacc ttcatgtcta
     4981 ccactgcacc ttgttctata gaaggtttat aacactcttt gtatttagaa atggcaatta
     5041 aattattttc atcatctaaa tatcctattt ctcttatcat aaacccgcct acacttgatg
     5101 gtattaaact ctctaatatt atacaatttg gtgcagtttc atctgtagtt gtatttccga
     5161 tattgccttc ccataccaca tttttgagag ctgtctgact ctcagttgga gtatattcac
     5221 tccctcctcc atcaccaagt tgaattttta caaatcccac tttattacct gtgacacttg
     5281 catttgctat ctttgctttt cctacatctg taattatagt gtaataactt ttatctatag
     5341 ccaatatatc acctcctaaa atattgttat ctcttggtat ccaactccat tgccagttaa
     5401 tacatcaatt tctccataag tttctatatc tggtggactc caagggtata tagttatttc
     5461 ttgacccatt agggttgtta taccaaaatt catataattg tctttgctta taagcactct
     5521 agtgtaatct aaagtcatat tacatggctt aatattactt acaaaagaat gaacttcctc
     5581 aaaccaatct tgatttctag catcactttc aagatgtata ttataagtag cattatttat
     5641 agttaactca taattgcctt ctccaactac attatctagc cagttcctta aaaatctctc
     5701 tgagtaaggt agtttactta tatatttact aaaaatccta aaccttctat cttctaaact
     5761 ctcattactt ttaggagtta tagacattat cttttcccat ctttttatac cacttatagt
     5821 taggtcctct aaaaactggt catttgatag gtcctttaat ttatcatgta gtgtttttat
     5881 ttctttattt tctacattaa atacttttat atattcttct ttatcttgta gaatttgtgg
     5941 taagtaattt attagattaa tctctttatc caactacctc acctctcact actatactgt
     6001 tactatttat tgttagatta gatttaacct catttatcat tgtgtttgta atatctaaca
     6061 ctccatctat actaagtaat ctagtttcaa tttgagatat acggactatt aagttttctt
     6121 catcttccca actcatgtta agttcattta aatagtcgtc tattgcttct tctgcaattg
     6181 attttatatt ctcccatgta tatccactct tatatgttat ctctgctgat atatttatag
     6241 ttgtacttgt aactccttca actgtgactc ggtgtccaat tggtgctaat ccaagacctt
     6301 ctcctttcat atttgggtca atttcttctt gaactaaatt aactaaatca gtacttggta
     6361 ctttaaaatt tgaattaatt attactaact taacagtacc tccaccgttc caaactggat
     6421 atactttaac tcccccaacg tcaggcaatt tattaacttc atccttatag ttttgtatat
     6481 tacccccaaa agattgagaa tttaaactat cataatatct ttgtcttaaa ctgtcctcag
     6541 attcttcatc ttctccattt atcaagattt cagttagttc agtagtttca agaccatcta
     6601 tatattcaat aggtattagt tttcctaact caaatatagg tccagcagtt tcacatttca
     6661 ttttatatgt tttttcagat attctctcaa ttgcaatata attgtattct cctagattaa
     6721 acctagaatc aagtggaata tctatattaa acactccttt tgcaatggta ttagttgcag
     6781 atagtggtgt aattcctcgt tctttacatc ttttctccaa gtaataataa ctagcagtat
     6841 ctacgaatgt ttggtctagt aattcatcca ttgcaatgta tgtttctgta agttctatag
     6901 caacaggagc aagagcatta tatattatag aaccttccct tttatcaaaa gtatctggta
     6961 cactatctaa cattctttta attatatttt caaatgtcat taactcaaac aattatacac
     7021 tcaccacctt ctctgctttt atatttccat attttgtatg aactgaaaat ctacattgta
     7081 ctttaccctt tatattttga aactcaaaat tatctatatt ttcaatccta tcatcttgaa
     7141 ttagtgcttc tgttatcctt ctttcaagtt ctggtattac atatgaaata ggttctccaa
     7201 ttaaatcatt taattcaaca ccataatttc tagaataaat aagatgctca tacctttcag
     7261 tatttaaaat taaaaaaatg gtttgtttta atgcttctac atcatcacaa ataccatcta
     7321 ctctattttt ctctatattc aatttaaacg tcttacttgg ttcttgtcta acatcaaaat
     7381 taattatcga tacatcttca atgtcataat ctaaattatc gcttggtaac accttatcac
     7441 atcctatcta aaatcaaata ttgctgtcct ccttgcatac gaattaagac taatttatct
     7501 cctatttttt tatctgtata ttttttgaat gtatctgttt gtattagaaa agattcttca
     7561 aaagaggctt tttgctctat cttaactact aaaggattaa cactttctat agttccaaat
     7621 gcaatttgaa ttggattact tgtttctact gcatccattg cagctttttt aattatttgc
     7681 aataattctt gactcatatt accacctcac ttaaaagaat cttctagctc tttcaaaatc
     7741 atgctttttc ttttgtctac cacttaaact acttattttt actacatcac cagtttgtgg
     7801 agcatgaata tattggtcat ttcctatata tagtccaaca tgatgtactt ttccttgttc
     7861 gtttttaaag aatactaaat cgcctgcttt tacatcattt atgttgcata atagtttccc
     7921 tctactatct tttgattggt ctgctgaaac tctaggtaga tttattcctg cacctctttt
     7981 aaatgcccat accattaaac cagaacagtc aaaactcttt ggaccattgc ctccccaagc
     8041 atatggctta ccaagttgat tttttgcttc ttgaataact ttacttactt tactattatt
     8101 gtttgttaat gtattagaat tattgttttg aacttgataa gttgtatctt ttaaattctt
     8161 ttctgcttct tcgttacttc caactcctgt acctgcatta ttagaagtat aggtacttcc
     8221 tgttatttgt ttataaaatt tgcccactcc tggtacccaa tctttattaa gagggctatc
     8281 ataaagaggt gcatatttgt ctctaatacg ctctaatgtt tttcttcctg tatggatata
     8341 atttcttgat aaattgctaa tacctttttt tatcccttca tctacagaac taaaagacat
     8401 ccctttcata ccaaagaaat tatttttatt tttgcaaagt gaagaacttc cattaccagt
     8461 ttcatgtata gaaatagcag ccattaaagc tgcattgact ttgtaagcat tagaatattt
     8521 aacaaatata tttcctgtat ttgataactt atttttaaat actttattta atttatttat
     8581 catatcattg tcttctttac ttgtagtact ttgtgcagga ccattttgtt tctcattttt
     8641 attattagta tttccactac tatatgagct tgaagaataa gaagcaaatt catctccatc
     8701 aacaagagtc aaatccataa agtgactgtt attttcaaaa gtatgtttta ctttctcaac
     8761 tagcatataa ttttgaagtt taatatctcc tagatttaaa aaaacaggta ctaaacaacc
     8821 tgctctcact ctaatatctc caagtgcatt ttttaaactt aatgacttag ttttcttatt
     8881 atatagtttt agaagtatat cacacttttg ttttatctct gcttcattca tatttttatc
     8941 aactgtctca aacatttgaa gtattcccca actcctcata tgtgtagagt cttgagcaat
     9001 atacacatct ctttttcctg actcctcgtt gtctcttaca agtttgattt ttgtataagt
     9061 atcactatct atacttgaat tatagtcaaa gtcctcaatt acatcattgt tcatgacagt
     9121 atccagtttc attgatgcaa cattctttaa tgttattctt ccaaaatcat catacaaggt
     9181 atacatttct tttttctctc ttagagtatc atctagtgct gttagtatca tgtcaaagag
     9241 tgttttattt tcttcgactc tagatagttt atacttagta tcttctatga cattgtattt
     9301 taaattaaaa tctttagcca acatttttac aagttcactt gctgttttat tgctatacac
     9361 ataagtatct ttgttcttaa aatatcttaa ctggtcgtaa gcaacaattt taatgtgatt
     9421 ttctttatct cttttcttct gaaatatata tccatagaat atacctacac ctttgtaata
     9481 tagtcttaca gagttacctt cacaaaactc taatatatca tccatgacta ttgtaaattc
     9541 aagtttagat ggtgttcctc gtctttctat ttcccatgtg ataccatcca aaactacagg
     9601 ttcgtagaaa tcttcccaat gagctattac taatcttaca tctctatcat ttgctaatac
     9661 taattcatca tccaagtttt aacacctgcc ctttgtaaat agtatattta ggtacttttt
     9721 tacccttgtt agctttatcc atcattgatt tatttaactc atataccttc ttatataatg
     9781 aaccattacc aagttgcttc tggcagattg accaaagaga atctcctgcc ttgactgtat
     9841 atgtcttagc atttggctta ttggatgaat ctggtctaaa ttcttttggt ttcatgactg
     9901 gagggggagt cctaccataa tttgtctttt caggtgttgc aagtactaac tttttagttg
     9961 agtaatctct atattgtttt aactttattg caacttttgt atctgaggca ttttctgcat
    10021 cttctactat gttatattct tctaatgaca ctttcatatt agtgttaaat aagaccttgt
    10081 tacctaactc acgagataca ataaattgaa atggcttaca atctgtcttt agtaattcta
    10141 gtttacttaa aaagaattga acatccctaa aagttccacg ataaaatggt agtttattat
    10201 gtgtaaactc tgcttcaaaa cttatttcag ataatccttc tttttttagt atgtttactt
    10261 ctccagtgtt tatcaaatca actgttttat ttttgtttgt cactttaatt tctaacttgg
    10321 gtggagggat tggtaattgt actccatcta aataaaagtc ataagccacg attacacctc
    10381 ctttctaaac tacgccttct gctgatacaa ccatggcgtc gtttaatttt tcagttaata
    10441 cattaactat tccatctaag tcagtatctt tacttatgtt gtttgtattg ttcatgtcaa
    10501 ttttaatgtt gactcctgta tacttgttta ttacttcttg ttcagctata tctcttaagt
    10561 attttaagtc ctcttgactt ttatccattg tctttgccat tttagctgtg tttcctgctg
    10621 tatcttttgc tccttttgca gcgtcgctga gcggagaatt aagccctgcc gaaccgagtc
    10681 cgtcaccaag accgtatttt ttgtcccaaa ggtcatctag tcctaaatcc tttttagcct
    10741 tttctgctat tttattgatg tcaaaagcat cttttaattt gttttctaag ttttgaccaa
    10801 atgcatatcc atttttagcc caatctccga catttttgta ctctagttgt ttaaaggatt
    10861 tgtatccctc tggctctgct ccaagccact tatttaaatc acctttagct ttttgtagtg
    10921 tacttgtaat agactttgta tgaccaacca tatcagcttt tccaagtcct aacttatctg
    10981 caagtcctcc aaatttattt aagacatcta caaatttatt ccaagctttt aatgccccat
    11041 taattccatc tataatagag tttgctaaat tagtagcaaa ttcatcacat ccttttgtca
    11101 tagatataag attatttaat actgctgtgg ctaaattaaa aaataatttc tttactgaat
    11161 aaactggatg gtctaataca tttgtaaaaa actcagcaaa tgatataaaa acattaatcc
    11221 agaaaaatac actatttata ataaatgctg ttaaacccga taatgctcct gcaacaattc
    11281 caattgctga ataagatgtt cctgcgaatt tatttattac agctatagct acaaaaaagg
    11341 caacaactat ggctataatt ccatataaaa tccaagtgat agggcaagct gccattgctg
    11401 cgttcaatcc gtcctgtgca attgtgcttg ctactaatgc tgctgctcta taagactctg
    11461 ctattatatg agcaaaattc atcgctaaag ttttagcaga taacgcaata tcttttactt
    11521 tattagctat actaagtaat agtgcattgt tatatactaa catagcagct gcaactccaa
    11581 gtactattgg tgcaataata ctccagttct gagcaaatac attagtaata tttaatgctt
    11641 gtgttattac ccagccaagc acttgcacta tcagactaac acctacaata atagcattta
    11701 caaatgcttg aaaaaatgga cttcctaata tatttataat tccattaaaa atactaaatg
    11761 caactgctcc taaaacatat aacgaatcta taaaattatc tataaaggtt cgaaaaccct
    11821 tactagacat agattgttct atttttttct gtataacacc aaatatcatg actgcattat
    11881 tcttgataga tgtgaaaatc tgtccaaatg tataaggcat cttctcgaac tctgcattag
    11941 tctgctccgc cgctgaaagt aaggagtttt ttacaatatc tgctgttaac attccttcac
    12001 ttgccatacc tcttatcttc cctatatcga cctcgagata atcggcaata gactgaataa
    12061 tattaggtgc tgactcaaac acagcattta gttcctcgcc tcttaacacg ccacttccta
    12121 aaccttgtgt aagttgtaaa agtgctgaat tagtttcttc tgttgttgct cctgcaatta
    12181 caaatttctt gttaagttgt tccgcgaatg ctactatttc ttttgtacta ctaaatgctt
    12241 tgcctgcatt catgcccact ctgcttacaa tttttgcagt atctaaataa gatgctcttg
    12301 ctctttccgc tgattgaaat atcattttgt tgagtcctcc atcagattgt tgcccgtcat
    12361 ttatcataac tagtctcgca ttagtactcg tcatttggtc acttaaatta atcaaaccac
    12421 caacacttct caatccaata taagttgcta ctaatctttt agcattttct actaacttat
    12481 ctgtactact tgctcctttt ctaatatcat cattaaatct tttttgttgt tcatcagctt
    12541 ctcttatttg ttgttctaat ctatcaaacc cagcttctgc acgtgctagt tcttctctag
    12601 ctgttctaat actattagca tctatagcat tgctagatgt tctttgtaat tgctcgaatg
    12661 aacttaatac aatattcata gcattagtca tgtgtctaaa agcaggtgtc attccgtcga
    12721 aaattcggat agatgtttgt atagttgcca tttttaacct cctttctgtt aatttagata
    12781 ataaaaacac ttactaaatt agtaagtgtt tttattatga atacatctat tttttgcctg
    12841 cccaaaattg tttgccacag ttcaaacatg ttactctaac tttctttgct cctaagttgc
    12901 ctgctactag accaatacct cctgctatag tcgcccctgc tacagctttt cctataccaa
    12961 accctttttt ctgagctgat agagatgttg aaccacattt aggacaacaa gcaactgatt
    13021 cttgttgagc tttttcaata ttattttttc tattttgagt atttattatt ttttcctttt
    13081 ctgaattgct catagtatca tcaataaaat tgatttttaa ctcttcaaag gaagaatcca
    13141 ctattttttt tgcttccttt atattcaaac cacttatttt cattacattt tttattgcat
    13201 ttactttttc ttttctatat tttctataaa tttcagtcat atttatttct attccatttg
    13261 catcaacgat taggtcattt gatttaatat tattatcttc aataatttca gtttctacag
    13321 caaccccaca acttgaacaa aacttgccta cgcctgtgat ttctgcacca caattcgaac
    13381 aaaacataac aaatccccct agcacaataa ttttataaga ttattatact atataagtaa
    13441 aatttttaca ttataatcac atcctttcaa taaaaaaaca cctactattc aagtaagtgt
    13501 ttttgattat ttttaatttt aagtccacat agttaatata aaacattttt aattgaagtc
    13561 caaatttgag aaaatgtgta atttacatac cacatagtta atataaaacc ttttgattca
    13621 atagccttct cgacctctaa tttagtattt acataccaca tagttaatat aaaattagta
    13681 gttcaatcct atgtagattc tcaaaattca ttatttacat accacattgt taatataatt
    13741 ctctatttcc attataccat ttttttacaa acaaagcacc tgaaacatca taatatccaa
    13801 gtgctttatc tatttattta cttatttttt ctctcttctt gttctctaag aatacctctc
    13861 aatatctctg catactcttg aaatttttct tcattgtcct gttttaattt atgtaataat
    13921 gtagcaaact ttacaaagta ttctacatct tcatcagttt ttaaattgta ctccttaagt
    13981 aaattctcat acattgtatt aatcccccca aaaataaact aaactattta atacaatcaa
    14041 atttactcaa tcttatacac tacatgaaaa ttcttctttt caccttcaat cttagctggt
    14101 ctattattct cctctatcca agttctaacc ttatctatta cactctttgt atatttattt
    14161 acagtaccag tccaagaacc attcgtttcc caaactcctt ttacttcatt ttcttctaat
    14221 tttatctttt taataatctc acaaacagcc aactgtgctg gtttattact cttagaatat
    14281 attttcagtt tagatgctat ttgttttgtg tcaaagaaag attcctcttc ttcaatttct
    14341 ataggtaaat caatgcctgc ctttttatat aatgtctttg ctgttaatag ttttgattta
    14401 ttatcaaagc ctgcaccatc taatagttct tttaacatag atgtactatt gtaagctaac
    14461 tgtaactttt caatctcact tgctttttct cttagttttt tgggattagc attgttagtt
    14521 atgtatgcac cagtttgtct tattgatggt agtacatcta ctgctaacca tgtttgaaat
    14581 tttactgcta tttcattttt agctttcatt cctaacatgt aaaatagtga ttctggtata
    14641 taatcatctt tcgcccactt gtggtcgaaa ccaaattcta tacaaaattc attcattctt
    14701 tcccatctta ctgatttgta ctcttttcca tttttatttt caattttaca ccatccaaac
    14761 cctacagctg tatcctctgc attaattcct atacttccat cttcatattt aatagttctt
    14821 atttttattc ctaattcatt attttcaaaa ttcatcagtt cattattcat acaaaaatca
    14881 ccctttcgat ttattatctt gaaagagtta tctttacatg atagaatatt tcatatagag
    14941 ataactctag gttaagtagt ccagtatgct ttggtcggcg ttgggctact tttttatcta
    15001 tcttcctcca aaagtaagtc tactcctttt cttattgctt ctgttctagt aagattttct
    15061 ttctcacaat aatctaataa ttttttatga gtattttcat caaatcttac tttgacatca
    15121 atatttttag gattatcaac ttttggtctt cccattttag aactcattgt ttcacctcac
    15181 tttttgtgtt ccataatata attatattat ttgtgttcca aaaagtcaat aacttatccc
    15241 aattttttct aactatttta ctcgaccgac caatttgagc aaaacaaaag cacctacata
    15301 tttgtaagtg cttactatgt ttatttaatt ttgatagtta gtatagaata aaatttctaa
    15361 tccacgaatt tcatggcttt gaatttattt atatgtagtt taattaatat aaagtagtgt
    15421 tcagtactaa attcgtacta agtgaatata aaatatatat ttttactata ttataaattt
    15481 aataattctt ttttcttaac atcaaattct tcttgcgtga tagcatccat atccaatagt
    15541 tcttttaatg tttttatttg ttctaatgga ttagttatct tatttttttc aacttcactt
    15601 tcactaacat ttccaattcc attatttttt ctatattcaa ttaaattctt atatacagtt
    15661 gctaaagctt catcattttt atcaaaagac aatataaaat cattacattc aattatcaaa
    15721 tattcttttt tattaagctc tgctttttgc attgcaagag caaatggacc aaataaagct
    15781 attctagttg ctgtatatct tctaataacc tcttcttctt tttcaataga aacacttttt
    15841 atatctgaat atgaaacttc aaatacatct actataggta caggtcctgt tccaaaagct
    15901 ttaacaacta tcttgtcact tctaattaaa agacaaatca tttcttctct attaaataaa
    15961 ggatgtcctc ctaaatatac aatttgtatt aatgtgtttt taacaaaatt tccaaaatta
    16021 tctctacata actctacagc attcataact tccttacttg tcagtttttt tagtccttta
    16081 ccaagaacag ctatttcacc tctgcatttt tcaaaacatt catcacataa aaactcatta
    16141 tctacagttt ttaaagcatt tccttcatta ccacaaatta tacaaatgcc tttttctttt
    16201 tccttctttt tgaataatcc catagcgtaa tccccctcat aaatatctat ataatcatta
    16261 taatttatct ttatacaaat ttccaatata aggtaaaaca aaagcaccta catatttgta
    16321 agtgctttct ttgtttattt agtttttctc cacatagtta atataaaacg ttggctagaa
    16381 aaggtaaaat caactgttgt tcttgttgct ttacattcca tatagttaat ctaaaacgta
    16441 ccacatttat aaccattttc ccaactataa ataagattta cattccatat agttaatcta
    16501 aaacagaaag attaaaaaat agaatgatac ttcttacaac atttacattc catatagtta
    16561 atctaaaacc tcttgaagag tatgctattt cagaagacgc agataaattt acattccata
    16621 tagttaatct aaaaccccaa aataaacttg gcatttccaa tacctacaca tacgcatctc
    16681 tctcaaattt gcagtgaacc atgagtagtg caattgataa catttatcac acaccctcaa
    16741 catcttagat ttcaagtgtt aagccatatt ttgtaacaaa tatcgctcac tgcaaaatct
    16801 ctacattttt attatatcat aaaaatatta tttttgaata tctgtaccaa tttgtggtat
    16861 aataaaagca aggaaataat ttactcctac aaagagtgat tattttcagt tgttaagtga
    16921 aaatattatt tttttaaatc acccttattg gcgtctgggt gattttttat tttgtcataa
    16981 atataagctg atataacacc agctagtatg cttaataaaa aaactatcat ataatttcac
    17041 ctccttcctt atttggaatt tggcgtttaa tatgaaaata atcacccttc gcactttcga
    17101 ttattatcct tgctacaatt attataacat ataattatta catatttttc catttttttt
    17161 atataaacaa tgaaattcaa gtaaataaat acctacttat ttatatatat tttataaatt
    17221 aattgcttta taatcaagtt ttcaattttt taataaaaat ttttattttt tattttatat
    17281 aaacaatatt ttttctaatt tgtggtataa taaaagcaag aagaactaca atctattttg
    17341 cggtagagtg aagttcataa ttaaatgaat ctatttgaac ttatggaact tgattttaaa
    17401 atcaaattcc cagccacttt tactcttgcc acgagttgag tggcttttta cttttagaaa
    17461 tactttacaa attaagcaaa atattaaact agcaataacg ccagctatta cattaagtaa
    17521 aaagttattc atacttccca cctcctttca ttaggaagta ggttttatct cagtatgaac
    17581 tccactctat aaattgtaga ttacatcttc ttgctaaaaa tattataaca tataattatt
    17641 acatatttta cctattctat atttattttt ttattttgct atcttcttcg tcccctcttt
    17701 ctctctcttt cagcttcttt cattgcttcc tcttcatctt ctatctttat aagtattgag
    17761 gcggctgcta acgctctctc attaacttct aaattcatat attcacttgg cttccacttt
    17821 aatttttgaa tacaataatg agtgatgcta gcatcaaaat cgccacctct gattagtttt
    17881 ttgcttcttc tactttatcc tcaaaagatg tatcaaatcc attgacttca ttcactttta
    17941 ctgtataatt gacatactca cctgctgtaa gcattgtctt taataactga gcttctccca
    18001 ttactccata actattttgg agttcggcat cctttaaatc tggaaatact gtagatgcta
    18061 cacataattc agctacataa ctattgtagt caatttcact tgtatattgt ccagtatgct
    18121 taccattgtt accaatcact tttactcttt tagtacactt tcttcttagt gcttcgtctt
    18181 cttcagatga taaaactttt aattcccatt caactggttt cccttcttta tctaaaaatc
    18241 tgttactcgc tacatatttt acattatcaa cctttattgc attttgactt aaaaaagcac
    18301 ttaaattact catattattc taatctcctt ttattttaat ttttcatata aaaaatacac
    18361 atatataatt tataaatgtg tattttactc cattcctgcc aataaattaa atttttctac
    18421 taattcccaa tcctcaaaag tgaaatccat atcttcatct aaatactcac catcagcatc
    18481 aaatttagta attattccac tgtccatatt acaatcttta agtactactg tctgtcttcc
    18541 tacagcagat gtagggtctt catttgtaac ttgtatgtca aaataaatat cctcaccagt
    18601 ttctttatat ctgtaaagta attctctaaa aatagaagta ttataatgaa atgttgcact
    18661 tccagtattt gtactcccag ttgttttatt tccctttgtt gttcttccta gaattggaac
    18721 ttcactttta tttttttcca ttttagcctc taaatctata gcttgcataa aattatatct
    18781 tttgccttct atagttataa aacattcagc tttctttgca ctaactgtat ctttagcatt
    18841 tattgtttga gccatattat cactcccctc tctaactaac tgaaactgtc atataaagct
    18901 tactcatagc atttataacc ttaacagcat cagatactat gacagttttc ttatcatttc
    18961 caagctctac actaacatca tcagttttaa aatcttctat tgcccttata ttctctaatt
    19021 ctttatggtg tttaacaaca tcattccaga aacttattct tcctgcctta tcattcggaa
    19081 ctttacctaa atacttttca ttaaataaag ttgcaatatc attagcaatt tggtcaagta
    19141 ctctaacact ttggttactt gaaaaatcgt catttttatc atctgtaaat gatacaaaag
    19201 tatttatatc ctctaacaca tgaacttcat ctcccacctt gtggaatata aatttaccag
    19261 tttttaaagc ttcttcaagt tgtatttgtg tataattaac atcaacatca aactcaccat
    19321 catacttttt attagtatta gatttattta tatcgcatcc tgctatagct ccagccgccc
    19381 aataaattaa actagattca actaaatctt tatctttaat cttattttct acagacacta
    19441 caccttcata atctgcatca cttttcttat atagtactgt ttgaaactta gctcctacct
    19501 tatctctcat tctctttgta aattctacaa ataaactttt aatttctgtt gttgtagcca
    19561 aacatcctaa agcattaaag ctatagcttt ctattttatc caagaaagct tggtactctg
    19621 ctcctgtcac agcttcgcca ttagttccac cagtaaatac aagtcctgca cttgcttcta
    19681 gtgttgcatc cttcttccaa gtgatatagt cattgtcttg taagtctgta ataacctttg
    19741 ctatttgagt atctaccttc ttattatcta aaagtgttac aacatcaaac ttagcattat
    19801 catctatatt tgttgtaact gttactttta agtcatttcc tctgatacca ctatattttg
    19861 ctgtggctat agtacaactg gctttaacgc ctttatttaa tttataaaaa tatcccaacc
    19921 ttatattttt gaataaatct ctcaaacctt tcagcttctc atgagtataa tcatatccaa
    19981 aatacttcac tgaatacttc tcaaaatcat cactggttac ttggaatact tcttcatcta
    20041 tgccccaatc taactctaaa ggtattgcaa caatacctct atccgataat gaactggttg
    20101 cccttgtagc acttacaaaa tttatatatg caccaggtag gaccttattt tgtgttacaa
    20161 atgttcctcc acctaaagcc atctagctca ctcctttcat aaaattattt atcatatctt
    20221 ccacttctga gaaagaatac aattcatctt cctttaagat tgcatttaat aaatcttttc
    20281 tatttacata ccttttagaa ttaactattt gctccttagt aaacttataa ttttcttctt
    20341 tacttaatgt tttactcaag tttatcacct ctctttagac caccaaacaa ctctactgtg
    20401 tccatcttat ctgtattatt gctttttata gtgaaatagt tataatcaac aaagaaatga
    20461 agaacattat ctacaatttc aaagttcata tttgtacctc tgactaaatc tccatcaatt
    20521 tctatatact ctaattcctc cagtagcatc tcagctacct catttatttc aaatgattta
    20581 tcattacttt tgggaaaata atgtacatca aaagagttct tttttaatgt cctgccactt
    20641 ggatatgata ctttgcttgg atttaaagga acaataaaaa aacaaggttc atttatacct
    20701 tgctccacat cttcactata aattgtataa ctctctccaa atgttttatc taatttaata
    20761 gatattccat caattatatt attaagcatc aaatactcct ttaagtaata ttaataactt
    20821 tttctctata atcttatcaa cttggctttg tagttccatc tctgaaattg ttaagaaatg
    20881 ttgtccttta acccaacctt ttccatcttt agttctatgc ccgaaattta catagcttgc
    20941 atattcagtt ggattaacaa cttctataat ataattatta ccttgtttgt aaacaggaag
    21001 cgacctagca taagctactc cactccaacc ctctctcaaa aatcctgtgt cgactggtgt
    21061 tcttctaatt acttttccta aaagtcttgc tgctaattct cttgctgcat ccttgcaaaa
    21121 cttatctaaa tcaatctttg taagcttctc caccttttta caaactcttt taaactctct
    21181 aaaatcaaca ctgccccatc tagccattat gctttatcct taaatagctc aagtattatt
    21241 tcttgatgat ttggatatat agctgattct ccacttctta catactcttt atcatttata
    21301 ataagttttg aacctgcttt aatttctata tctggagata taaagagttt aatagtttgc
    21361 tctagcttag ctaatttccc ttctgtagca gaaactatat ttttatatga aagtttacat
    21421 ggttgatttt ctaatacaat cacttcttta ttgttagttc gttttgttac agggtcttta
    21481 attggctgat actcaactat agtacattta tctctatata acatttctat tgcttttcta
    21541 gttttactta ccatcttaag cacctaaagg ttaatatctt attcttacca taagtagtaa
    21601 gataagctat taagctatca aagcgttgtt ctggtgtttg agaaccactt cctatagcaa
    21661 aatctacctt tgtatcacct tctgatatag acttttctac agcttcaaag ttaatgcttt
    21721 ctatatctaa ttgacccata tttttcttgg taaataaaaa ctctccaact atcatatcaa
    21781 cttcaatttc tttcaattca attggcatag tttttatatt acaatctagt ttaataatat
    21841 tttctatttt ttctcttaca aaacctatta accacttatc tccatctttt aatatatatc
    21901 caaaactttc aagtcttttt tctatttcat caattatatt attttccata attttcacct
    21961 actttttagt aagtttattt ttctctttaa gctgcttatt tccttcttct aaagactcaa
    22021 cttttgacct taaaatatta ttttcagcta ttaaatcttt tacatttaat gacttgccat
    22081 actttactgc cttaccagtt tcatctatca aatcatatcc catctctaag aaatcatcta
    22141 ttttacattc ttctatagtt aatattctat ttaatttcct tacttgtgcc attatgctcc
    22201 agctccttca acaacaaatt gtattgcatc agctttttta tttaatataa atacatcctc
    22261 aaaactttct tcaaagtaga agtattttcc ctctgtaact gctgttggtt cgtctaactt
    22321 agagaactga taagaaacag gtgtaattat tgcacttggg tgaactaagg acataaagat
    22381 ttgtttagct cctgctccta ctttccatcc agttgtaaaa tcatatgcag ttttcattag
    22441 attagatggt actttaatta ttttaactgt gtcaatatca gttgtttgac gattaagaga
    22501 agttcctgca tcctttatat ttactgttct ttgtatctct tttgcatttt tgataagtgt
    22561 atttactact ggagtaacat acaatattct tccattttca ggtactctag cttctgtcat
    22621 tttttccatt aacttatcaa atacttctaa tacgtttgtt gttgtaagaa cagttgtatc
    22681 tgctgtatta cctaatgcgg tccaatcagc atatatttta gatatacagt aagcatccat
    22741 ctctggaaac ttttgttcct cattatatac ttttgttata ttgcctattg aagccacata
    22801 attagtttgg tttatatctg ctggatgaac caatgttgac catttccttt gattagttaa
    22861 taccttaggt tcccaagcat tatcatagtt tctttgagct actgctattg tatctctgtt
    22921 tgaatctact cttccagttg tagatatagt tggtatttct attgttttag aaccagtcca
    22981 tctatatctt ccattatttg gtgttgcata caaatccccg aagtttaaag tataaggata
    23041 tgcttgtgct aaaacatttg aatattcttt tgcataattt agtgctgcca ttttatttcc
    23101 tcctatttat tattattttc atgaggtctt accccagtaa aattaaaacc aaaatcattt
    23161 atcttaggct cttgccctgg tgttatagta tctattttag gctcttcacc ttctagtgtt
    23221 gcattaaaca aataatcttt atcctgtttc aaagggttta tttgctcttc aaaagctttt
    23281 tgtctatctt tactatttct tagtgcttct atatctaaat gagcttttaa tgctatttca
    23341 tccctacatt taatagactt aaaagcatca tttaaccagt aattaaagtc cttttcttca
    23401 atttcttttt tgtaggtttc ttccaaagtt ttcttatcag tttcataagt tgtttttaga
    23461 ttctctacat cttcttttgt catacctcct tcaaactttt taatagcttc attagctgta
    23521 ttaagttgtg tttcaagatt tgtataatct tcttgagtaa ttgtagtctc ttttattttc
    23581 ttttctatag atttttgaag agaaactaca tcaattttgt tattttctat ttttattcct
    23641 tctagcaatt cttctaacca atccatcttt taaatatctc ctttcatttt ttacacaata
    23701 aaaaaagtct ttaaagactt ttgtctagtg atttatttaa ttcttctcct actctcttta
    23761 gaatttcatc agaaacttta tctatattag cttcattttt ttcttttaat ttattgcaaa
    23821 gaatacctac attgacatta gcaatacaat taataaagat ttgtactaca caaactacag
    23881 ctataacttg agttaatatt tacattaaaa ttacctactt ttttaaactt ttacataata
    23941 aaagcaccta ctaatttatt gttcagtaag tgcttctaac attgataact catttctttt
    24001 ttcaaatcgt tatttttagc agcttttata agtttagaaa tagcttttaa cgttgctgat
    24061 ttacttatat gttcatcttt tgactctttt attatttcat aattataaaa atcatctaaa
    24121 tatattttta ttcttccatc aagcttattt tcttcatatc cataactata tataacatat
    24181 tcattattct tttctaataa gttcattaag acaatcatct tcttcaaact ctcctttctg
    24241 tttaattaat tctttatacc aatcatgctt tttctcagtc attctatggg cttctaagtt
    24301 acttatatta tactttttct caaatatact ttcaaggtat tcatgtttta ataagagaat
    24361 atcaatatct tcgtatgtac catttataag tctttgccaa gcaactgaca tactatagtc
    24421 agaatccaac atacgtctag ttccatctct cattatatga gtattgtaga aaatatgatt
    24481 tttaatttga cctatacttt tttctgacca atttgtattc tttgatattt tggaaatatc
    24541 atcttttcta tttctaatat tatcgtaata tagttctgct tcttcttctc ttcttatatt
    24601 ccactcgata tcacctttag tatattttgc accagtttgt ttactattat ttttaacata
    24661 cttctcatac cactcattat acttcatact agatggtaca taatatgttt ttccatcttc
    24721 tcctcttgct gctctatagc cttcttcatc ctcgaaccaa ggagctgttg ttgtcctaca
    24781 acgacaatga aatggtggag ctgttattcc aacttgataa tctttcatat caaatatttt
    24841 tccatctaac tctctgcata tatttgaagt tcttaaatct aatgtagcaa taatctcata
    24901 tttctctaca tctaaatcat taaaacaatc ttttcttgaa gctgatgcaa agaaagctga
    24961 ttcagtcatt attaagttct tagcttgtga cttagatacc ttaaatctct tagcaaagtc
    25021 atttactagc ttctttggat tttcacctct aataattgat tgagttagct tagtatgtaa
    25081 ttcatttact aaagcaggtc tatgcttacc ccaaatcctt tcactgaagt ttaatccatc
    25141 actagtccaa ggcttagaaa taactttatt tattctgtta gtatcaagac tcattaaact
    25201 ccaaccaacg tttactcctt gttgaacatt aaaagctgta tgatagtatc cacttgtata
    25261 aatatctctc attagtttat caataccatc aagttcattt ccatataaaa cttctacttg
    25321 ttgctgtatt tgcagtttta aagcttcaag ccttgttata tgaactcttg cactagcatt
    25381 ttctaactct ttcatccact tttgatttat agcattttct ttaccatatt taatatattc
    25441 ttctacactc catttaaact cttctagttc tcttgtattt agcagtttct tagcttccaa
    25501 taaagatatt ccctcatttt cagtgaatct gttataccat ataagtatat ctctttctat
    25561 attagacatt gctagtttat attgtttttc taattcaaga taatatttta tacttttatt
    25621 attctgagct tcttctaatt gttcaaatct cttcctccaa taatctttat gtttcatcta
    25681 taacaccatc ttgattatta ggaattaaat catcatactc tttttgagta tcttcctgtt
    25741 ttttaagtct ttcaagttcg tcattaacat cctcgaccca aggatggtta gaaacaatag
    25801 tttcatctga tacaattcca gttgatttag ctgccatatc tatcttttca gcttcattta
    25861 ttatcataga gtgattaaaa gtaatttgaa ctgttttata atcatagctc ttactaccac
    25921 ttatctttaa atactcacac acaaaccata aaagctctct aattgctttt ttaaacttct
    25981 tttcagtctt agaacatttt aagtcaagta gtgaatataa aaatttaagt gctacacccg
    26041 atttgtcacc tgtgttttga gattctggat taactccttg accaaagata attatattct
    26101 tttccaatct atcaagaagc tcctttttag cttcaactgg tatatttatc tctagtttat
    26161 caactccacc tccaccatct actttaattg atttatagta tcttatatta tctataaact
    26221 cttgtagact tgttcctgga tattctttta atacataaat aacctcttgt atttcatcta
    26281 agttatctgc tagtgtagaa atattattgt catatatatc tattaatgat ttatagaaag
    26341 ttaaatctga gacacacttt tcattatttt taaaaggtat aaatggaact ttaccccaac
    26401 cctgttcttt gttatttatt ctaaaatgac cttcttgtat atcagtcatt tttccatatt
    26461 catcatataa aaattcttga acaaaactat tacctctttc aacaaagtaa gttacatcat
    26521 tttctgtgta gtactcaact ctttttattt tatttccatc tatatcttca atataataaa
    26581 acctaataaa tgcaactaat tccctctgtc ttttactatc ccaaatagga attgcttctt
    26641 cagctggaat tattacatat ttaaactcac cttttctatt aatatatgga tgtaaccatt
    26701 caaccccttt attactagca ttgagatata gttctgttat tgtatcgtca aactcttctc
    26761 ctagtaagtc atttaaaagc ttagtgagat tatcatcatc tgcattaaat acgattggat
    26821 ttccaacact atagcccact ttttggtcaa ctaaaagctt atggtagttg ttaattgctt
    26881 tattattaac cttagtaaaa tcatcaacct tagctccatc taagagataa tatcttctct
    26941 tattgtttac atcagtatta ccataatagt attcttctcc ttgtttatat ttttctggcc
    27001 tatgctttaa gatgtagtgt tctatgactt ttactaggtt aaaggtgctc tcttttttta
    27061 actgaacttt tattaaatct gtttcactta tataaatatt taacacctcc tttactttaa
    27121 gaagcttatt ccattatttt taagcttatt atctatagaa tatctaagcg cagccattgc
    27181 atcatccata aactcaactg gttcatcaag atataatcca gttctttcat cttgtttcca
    27241 tttccattgt tgtatttctt ttatggtatt agtgcagcta ggatgtacat gtattcttaa
    27301 ttgtttcaaa taatctattt gagctttaac acttcctggt ccttttttaa ctcctttagc
    27361 tttatatcct gcactcttcc acatcttaat tctatctggt tcagcactat cacagtacat
    27421 aaatagagtc ttttctaaac ctctactatt tgcaatcttt atgatttctg aagtatccat
    27481 ttcatgtaca tatatttcat tacatatata taattctcca tccttaaagc caattctaag
    27541 tactacattt gcatggttaa atccaaagtc ttgtgataac ctcatattgt caaaatactc
    27601 aaattctgta ggaaattcat gtataacata atttttaagt attgctccac cagtttctcc
    27661 ccattctcca agaccataga ctttgtaccc ttctgggtct tgctcttttc tcatttgcat
    27721 tcttctgtag taagcttcat ctatgaatct attttgtaga taagtactat gatgagtaaa
    27781 tatatcatca tttttatagt caaaatactt tctttttatc caatgagtag ctgagactgg
    27841 attaaatgta aatgtcattt gataatacaa gttaggatta gttaaaatac ctcttaaacg
    27901 gtcatctagt atgtctatgt cactttccat aagttctgta gcttcttcac accaaaccca
    27961 tgttaatttt cctttcgaga agttaattga ttttaatttt tctctttgtt ttgcatcatt
    28021 aactcctctg aaaattatag agttaccagt aactttactc ttaatttcta aaggatttaa
    28081 agtagttttc caatacttat cagcttgttt accataaata cgatttatag ctcctgtaag
    28141 ctctgcatac gttgaatact tatgtgtagc ttctgacttt ctaactacta atagattagc
    28201 tccttgatac ttcttatctc ctaactttag tatatagtct tgtgctacat taacagattt
    28261 tccactccct gctgaacctt tcattgctct gtatcttttt ttagtaaaat tagcttcctt
    28321 gaaatctgga ttaaaattta ctctaactat catttctatc accataatct acacttattt
    28381 tcaactcatc atctccaata tcatctttac ttaggttatc aacttcacat ttcaacttct
    28441 caactctatt tttctgctcc tctgtagcca aattccaatc cttatgaatc atttcatcat
    28501 actgtttaat taaacttcta agttcactca tagccctact ctgtgcatta agaaaagatg
    28561 cttgcctatc ccatgcaaat tgaaattcat actctatctt ctcaccattt tctgtgcttt
    28621 catatttctt taattcttta accatttctt ccttgccttt aacatacatt atcttctgtg
    28681 ctcttattat tgctgcgtat tgaattgtta tctgttccca aagaatatca aatttatctt
    28741 ttatagatat ttcttgtatc aattccctag tttcttcggg tagatatttt gagaagaaac
    28801 caaacttttc agcgttctta ttctcttttg gagcaccatg accaactgaa tttttattag
    28861 aaaagggtgc acctctttta tttataggtg cacccttctt tttttcacta gcccagttgt
    28921 atctttttat ccatgacttt aaagtgttta agctaatgtc atactttgat gatatttcct
    28981 tttgtttcat tccttttaag taatcttgtt ttaccttttc tttgacatct tgcacatcac
    29041 cacctcttta tttgtttgtt ttgggaataa aaaaaacaat cctatttaat aggattgttt
    29101 caaatctagt ctcctctatc cttccaggat aaatgaaaca tacttattat taatgtagtt
    29161 agagataaaa ttatatgaac tgtaaaaaat attaaaacat agaaaattat attagaataa
    29221 tatcgagtaa gcattattgt attgataaaa catattacaa caaccaaaat ctccattatt
    29281 acagtataat atgcttttaa ctgtatgaat tgaaagtatg atatattaaa tccatttacc
    29341 tttctttctg tttttatatc ttttgcttct gatattgatt tgttatatgt agtaaacaat
    29401 aatgtaataa tgcaaactcc aaaagctact aatagtcctg acaaagttag tacatctttg
    29461 tttaaagaaa ttacaacttc tttattaatt gacgaattaa aaaacatgta tgaaataata
    29521 catattaata ctggtgttaa aactaactct actacttctt tagaatttat agccttgtaa
    29581 taattgagta agatatttaa taccttttta tacattttaa cacctccttc gcaatctatg
    29641 cttcactcat agcaacttct tgatacatta aattaaaaaa aactgaatta ttctcaatta
    29701 aatcatctat caaattattc atcttatcaa aaatgtcttc actatctata tgattatcta
    29761 tatcaagaga aacctctatt tctctacaca actttatacc ctcagtatcc aactttaatt
    29821 ttctcttgtt tttctctccc tcaattatta tcctatttat ttttttattt ctaaattcct
    29881 ctcctttaat atatttttcg caacatcttt taacttcact aggtaaatat ctagttctaa
    29941 atgtgggctt atatatcaaa tcgttatgtc gtcttgaatt atttaaatta gaaaacaaat
    30001 tatcttcatc agttccaaac ttctctctat caacatttaa agttattttt gaaacaccat
    30061 ctaaatctaa tatttgttct ataaaatctt tacttgctat ttgtgtaata ttaattgatg
    30121 tttttgacat attattatca aatttattat tttcaatcat atcctcataa tatagatgta
    30181 agtttttact tagttcaatc atgggaaccc ccacaaaaac tctttcaaat aatactactg
    30241 ctatattatt ttccttcata aatttaataa ataggtgctg cttattgcta tctccttgat
    30301 ccattttttt attaccctct acttctttag tatgagcatt tataatattt gtattctgat
    30361 tgtatttagt atattcaagt ttcaccttga ttattaaatc atcctcgcta cattttactt
    30421 ctgtaatcca aaacttttta tctttagtaa tattaattcc atttttgttt tctttacaat
    30481 attctttatc cttaaagaag gtctttatta cctcttttaa aatttcttca tctgaataga
    30541 cactcccatt aattttattt ataatatcta ttctataaac atcaatatat ctttttttaa
    30601 aattactcat tataccatta ttcctttcac aaatatttat aatgatataa ttctatataa
    30661 tcagtcaata tccttcaata atcattcgac aaccacaaaa taagtctaaa taatactttt
    30721 accttattcc tgtctataat gtacaaatat atttcatttt taattcatta cataaaaaaa
    30781 agaccctcca ttaatacagt catttaaatc atttctatta attctttaat cttttatata
    30841 cctctttata attcatactt ttatctatca acctaggtga tttcatagat ataactcttt
    30901 caagtgcttg tattaataaa caaactcttg tatttcatct gtaaaatctt ttctgacctt
    30961 tttaccttct gttcttatat ccagccattc ttgatctttt tctgtggcaa tatagtaacc
    31021 atgtattcta atttgtttaa gtgcttttgt aacccacttc gtaaatagtt ttgcttctgc
    31081 tttattactt ctaaatgaca tattgtacac agcttcttct gtaacaaaag tagtaccaaa
    31141 gttaggcaat ttatctttaa agtttctagt gtaggaatct ccgacagtag actcattaat
    31201 ttctttttat attctctatc tatatttctt aatgtatcac gaatatttac tatgcctagt
    31261 tcctctccta cgtcatttgc attaaatcaa acttcttctc cattttttga ccatatcatc
    31321 cttacatttt tctcttgtaa aattttcaac atactactac ctcctaaatt agttattaat
    31381 aaagaatatt caatttgaac acccttattt tcgacccttc aatatgaggg attaaaattg
    31441 tataaaaaag acctagaaat cactctaagt catgaattac tcatttatca ttctccttat
    31501 ttacaaaata aaaaagacta agttggggta acttagcctt tttcaagggg gaatatatta
    31561 tacacttgtt tcatactacc attataacag ggttaaaata tcattaaaat atcatctttt
    31621 tatcatatat ttatcaaaca gttaatttca agccatctat accgaataaa tacactccca
    31681 gttcctttat catttcatta atccaacgtc ttgatgtaac tactccacaa tttaatatct
    31741 ctgctatttc ttcatatgtt ttttcatcac aatagtaaag ctctaaagct ctatactttt
    31801 cataagaatg taaacgctct tgtcttaatt tcaaagtttt cattgctgaa tctatatgtg
    31861 ctatcataat aatagttctt gctttacttt tctttatact taaaatatac aactcttcat
    31921 tatcaagctt acctaaatca tcctctaagt aatctacatc tttagcttca cttaccgctg
    31981 agtctatatg tctcttaaaa tcattataat gcttcatcaa taatctagta ttatataaaa
    32041 cgcctttttt cctatcttct ctttcttctt ttcttaactc tcttacaatt tcttttatac
    32101 tttctttatc cacttaaatc actccttctt gctctcttcg aacaacttct actgcaatac
    32161 ttaactttag atttatccaa cttataaaat cttctaccac accaagcaca tcttactatt
    32221 ttacctgggc ttataagttc catcttttct ttttactttc ataccttcac tccctatatt
    32281 cttttaattt tgctttgact gcttcaagta atgcactttg tcctttatct ttattctcta
    32341 gagcctccat tacttgttca tctattgtac ctttacagat tagatgatgg ataataacag
    32401 tttccctttg cccctgtcta taaagccttg cattggcttg ttgatacagt tctaagctcc
    32461 aagttaatcc aaaccaaaca attatacttc cacctgattg taaattaagt ccatgtcctg
    32521 tacttgctgg atgacaaagt aataattgta ttcttccatt attccaatca tatatatctt
    32581 ttgagttttc tattgttctt ggtttcaaac tcttaaactc tttcattaaa cgattataat
    32641 catgtttata gttataaaaa actataatag gcttaccatt tgaaacatct ataatttctt
    32701 ttaaagcttc taatttttct ctatggagtt cctttacatt tttatcatta tcataaattg
    32761 ctccattagc taattgtaaa agtttatttg ctgctacagc tgctgatgaa gctgttatta
    32821 tatctttttc taattctaat atcttctctt tttctaattc tttgtaatat ttaagtattt
    32881 tactatcaag gtgtatttca attttattat ctattttttt aggcatattc aaataatctt
    32941 ctgcttttaa gcttatacaa atatctttta ttttattatg tattgcattt tctgctccat
    33001 ctttaggctg ccaattatat atagctcctg tttgataatt tttttgtcct ggctcaaagt
    33061 atcgttcttt atatcctgta atagttttac ctagtctttc acctctatca agtaagtaca
    33121 tctgtgccca taagtctatt aaactatttg gtgttggtgt accagtaagc ccaactactc
    33181 ttttagttaa aggcaatact ttctttaaac ttttaaatcg ttgtgcttta tttgatttaa
    33241 aagaactaag ttcatctatc acaaccatat caaatggcca ttttctttta taaaaatcca
    33301 ctatccaggg taccatttct ctatttatta tataaatatc tgagtctgta cttaaagctc
    33361 tcactctatc taatttacta ccaagaactt tagatacttt tagatgcttt agatgctccc
    33421 atttttttac ttcactactc caggtatctc ttgcaactct taaaggtgct ataactaaaa
    33481 cttttgatat atcaaagtaa tcatacatta attcacttat agctgttaga gcacagactg
    33541 ttttacctaa tcccatatct aatagtaaac ctatattatt attatctata gtttttctaa
    33601 ttgtgtattc ttggtatgga tgtggtttaa attccatccc ttaacctcct tgataaaatc
    33661 atctatctct tttaatgtgc ttacacactt aactttaaat cctaattctc ttaattctcg
    33721 cattttatat tgttgaagct ttcttagttt tttacctggt gctttaagtt ctacaaatat
    33781 aacatgtcct tctggtaata agacaatcct atctggcaca cctgcctcac ctggtgaaat
    33841 aaacttcata gcctttccac ctaacaactc aatctctttt ttaagtcttt tttctatttt
    33901 tgattctaac aaaatatcac ttccttaaaa tttaagtgtc aacaatgtca acagattttc
    33961 tatatatatg tatatataca tattaggcat acatatgtat atacgtatat gcctaatatt
    34021 acttatacta ctttatactt aatttttgtt gacattgttg acattaatac ctcaaacatt
    34081 gtaattactg catcttaggt gtcaacataa gcgtcaacaa agccattttt tttgttgaca
    34141 ttgttgacac ttttaaaatt ttcaaattta ctctattttt aattctgttg acactaactt
    34201 ttacgtacat aagctctctg cctaccatat attttcccaa accttaaatt tccattatat
    34261 gatatccaat catctaatcc ttttaatata tcattaattt ctcttgataa gataggcgtg
    34321 agttgtttag gttctccatt aaatagctca acccatatct ccataacaca cgttttttct
    34381 cttaatattg tcccttcttt taaatcacca aaatctgaac catgaatata ttctctcttt
    34441 tctgaaatac ttaaatcata ccaattctta gtaataggtt tatttagata ctcttcgata
    34501 attccagatt tagcattttc ttctgagtga gttctttgtt gtctttcagc ttcttttttc
    34561 tcctcatctg atagatatag ctgttcattg gctttatata attctactgc ttctgcccaa
    34621 atttgatttc tttcattatc aagttggcca ttaaatatac tcttgttagt cttctttact
    34681 cctgcatcta ttggccaaaa ccttctattc ccagtcttat ctcttaaaaa ttctttatca
    34741 ttagttgttc ctataactac acattgacgt aaaaatcttg aggttctctt cccatatgcg
    34801 accctatata tatcctctga tttgcttaga aaatgcttaa ctgcctcaat atctgctttt
    34861 ttagtagcca tcatttcacc catttccagc aaccatactc cttgtaattg ttcatacgct
    34921 tctttacctt gtacagtagt taaactatca gaataccagt ctccaccaag ttttttaata
    34981 aaagtacttt ttcccattcc ttgaggacca gataaaacca tcatattatc aaactttatt
    35041 cctggattaa atactcttgc tactgctgct actaaaactt ttcttattat agtccttgta
    35101 tagtggttat cttctgcacc taaatagtca attaaaagtg tatccactct cttaatgcca
    35161 tcccacttta aagaatttaa ataatctttt ataggatgaa aagtattatt ttcaaaagca
    35221 attattagag catcatttac ttttgatggt gatgagatat tgtaaattgt ttctatatga
    35281 tgtctaagcc cagaatcatc actatcattc caatcattta atttatcatc ttttctccaa
    35341 ggtaacttac ctaaaacgac agctctattt gaaaattcat tataagctat ttttcctttc
    35401 aaatatgggt cattttctat aaacattaag atattgtttg ttgttttctt ataacttccc
    35461 ttattgtcat aatctaacct agttaaccat tcatcatctt caaaatctat atcaccaaaa
    35521 tcatccttag ctttatcaag gttttctctt cctatagtct ttcgtacttt agtgtcactg
    35581 cttgcaaatt cgctcattcg agtaaatgaa ggtaatctat ttacaggcgt ttcgggttta
    35641 gcatcttcat caagttcacc aaatttatgt atcctaacta aatcaaaagc attgcataaa
    35701 attccgcttg ctgggtctgt accatgatga ctatatgaaa acttgtcatc ataaataact
    35761 actccaccac ttgtactacc ttctgaatat gtatacctgg tttcatcaat accaggaata
    35821 tatacttcat ttaagaaagt ttctatagtt tcttttatac tataggacct gcaaaatgca
    35881 cctataattc cagccttttc aatagggtct tgttgttttt ttaattgtgt attaaacttt
    35941 tgcctctccc tcgaactttc tggccaatag cttacatctg tccagtctaa atataaatct
    36001 agtatttcat ttgggtttaa aaactctcca tcttgaaatt taaatatata gtctccatca
    36061 attgaagtac ttggaaaata cataagtcta tgaggttgat atgtagtatc atcaaacata
    36121 tctattccta tagtatctgc tatcatcctg gaaattgctt gatattcttc tggtagtact
    36181 ggtctagtta aaggtattac aagtctatat ctgggattat tctcagtatg tgagtgcgta
    36241 gaatacataa gacaagcata atcatttaat aatgttatat cctcccatat atctttattc
    36301 gcataatcta tatctaaagt tataatactt ctatttgcaa tattctctgc ttttcgtctt
    36361 ccatttttta agcttccacc tacaaatcca ccaacatctt taactctatc tttttcagtc
    36421 tttgacatct tcctatattc tgtatatgtt tcttgagttc ttaatgtttt acttaatcta
    36481 tttacaagtt cggaccataa aatactttta tttttccaat gtgtttctaa tttatttttt
    36541 cctatggcca gcatgagttg gccatcatgt cttacattta tgtgttcaat ttcactggcc
    36601 tttatatcca taaatcaatc acctagtctt tcttataata atcacattca tatccatctg
    36661 ctttaagagg aagaccttta gcccaagata tttcttttcc cataatactg ttaacttctt
    36721 ctaaagaacc tcctttttta tctacatcaa ttacaagctc atcatgtaca tgcattacaa
    36781 tgctataacc tgcatctgtc acattaaaca tagcctctct taagcaatct ctagctgtag
    36841 cttgaacaat attctcaact aacttaggtc cataagtatc tattcttttc cattgtttac
    36901 ttgtctgttc cataccttca tatgttatct tatccccact aaatgtagta tgtggctcta
    36961 tcttaggcct tagatacgat aatcttctac tacttggtag ttctataaat aaaacgcctg
    37021 gattataaat aaatttaatc ccatgttgaa tttctactat agtcctatct tttatagctt
    37081 ttttagctgc cttatctaca tcccaccaaa acttagttat atttggatta gcatttctcc
    37141 atgcagtaac aataggttga agctcttctt ctttaagccc cattttaata gcccccatag
    37201 aaattaaggc ccctacactt ccgccatatc cattcgataa ttctgctagc tttccttttt
    37261 gtctaagttc agaacctttt ttaatatttt cgattggaat tttaaacatc tgactggcac
    37321 tagcttcata tatttttcca tgagaattaa acacatccag tctccacttc tcacctgcaa
    37381 gccaagctat aactctagct tctattgcac taaaatctga tactataaat ctatgacctt
    37441 cacttggtat aaaagctgtc cttatcaatt gacttaagac atctggtaca ctatcatata
    37501 aaagctctat taaatcaaaa tctccttctt ttaataggtt tctagctagg tctaaatcct
    37561 ctatatgatt ttgtggtaaa ttctgtactt gtactaatct tcctgcccat ctaccagtcc
    37621 tattagcccc ataaaactgt agtagacctc ttactctatt gtcattacct ttagctaatt
    37681 tcatagcctc atatttcttt atagaagttt tggacattaa ttttctaagt tctaaaattc
    37741 taactacatt ttcatcatca acttgtttta atatttctgg aatactttct tttgttaggc
    37801 ttgtaatctc aaagccaact ttatcactta accatttttt taattgagct ggactatttg
    37861 gattatttag accagttatt ttaattgctt cttttgtaag tttttcagta tatcttttat
    37921 cacattctat tgcattctct attaactctg tatccacttt aataccagta tcattaattc
    37981 tctggtctaa ataccataat ttaatttctc tttcggtagt cttatactta ctaagtttgt
    38041 ttcttatttc tctttcaact acaacatctt gtttacaata ttctttaaat ttattccatt
    38101 tttccatatc atgtattggt aaatttctag ttcttccctt gttaactttt gtagccttac
    38161 aaggtttaca gaaatattgt attaatgctt taccttcttt cattttctgt ttatcttcat
    38221 taaactttaa agccttagac acactatcta aacttcctgg aagtcctagt gttaacgcct
    38281 ttatcattgt acatgaccac tcatttggtt ttaaattaat atttaaaaat ttacttattg
    38341 atgttctttc aaaattagca ttaaatgctg attttataac tttattatca tttaaagctt
    38401 ctattacttc ttttggtaac tcttcatcat ttactaaatc aataactttt acctcttcat
    38461 tatcaaaagc ataggcaaat aacagtatct caaaattagc agagtctaca tatctgtaga
    38521 ctccaacttt ttttatatct aaatcactat atgtttctat atcaattgat aaggtcctca
    38581 ttaacttaag aagtcctctt cttcatcttc atattcaaag tcatctgaga aatctgcttc
    38641 tgcactagct ctagctcctc caagtacttc tccatctgct aacttttgaa cattttgtaa
    38701 gccacaacct attcctttat ttcctgcact attatatggg aaaaagttta tactaactct
    38761 tccataacaa ccactatata cctctgtatt atctaatatt tcatttaagt ctttatcaac
    38821 tattcctggc ttttgagtac tatttgcatt taaaaaatac attcctacat attcctctgc
    38881 ttcatcagct ctttctgcat ctccatcacg aagaggtgtt tttaaattac ctggtaattt
    38941 tccaccccat ttagaagttt taccttgttc tttagctgca tcaatagcct tctttattct
    39001 tcctaaagtc accttatctg attttggtat taaaatacaa actgaatact tgggctctgc
    39061 accttctacc attgctctgc ttttaaagat attgcaataa cttaatctta cctttcctgt
    39121 tactaccttt gttgattgta ctgaattact cataattttc ttcctcctaa tatttttata
    39181 tttggtatat tgattttata tttgaagtta tttctccaaa tttatctaaa tctctaactt
    39241 gcttactaaa caatttaatt cttccacaaa taactgttga agaaacattt aataactctc
    39301 ctatatcaga tatcttttta tcttgtaaaa tccaatctac tattttatca atatctttta
    39361 aatatgactt tcttgcaatt tttcttatat aatcaacatc atagttaaat tgaatttcat
    39421 aaaatccatg tatatctttt aatggtcttt tactatcttc aaatacactt acatctaaac
    39481 cttctataga gtttctataa tgtttttcgc cataacgcaa agctttaaac atttctattc
    39541 ttatgtaaga aacagccact gtagaaaact tgcctctgtt actatcataa tttaatgctg
    39601 ctttatataa cccaatacac ccttcttgat aaaactcttc atataagtat ggatgttgct
    39661 ctacataaga cttaaaaaat ctatttatac taaaatgtac aagattcaaa ttattttcaa
    39721 ctagtttagt tatttcttga ttactcataa aattatatcc ccttttaaat ctttttatag
    39781 ctactaggaa ttaaatattt ttaattatcc tttgctctta ctgatacaaa gcatactgga
    39841 ctaactttta tatgttctgg tatatacact ctatcaagat gattgtcatt tacttcgagg
    39901 aaagttgaca tcactttatc ctttattaaa ctttctcttt taaaatccat cactataact
    39961 tttgcatttt cttcagttga gttatattct gtaatattta gtatctttaa catatctagt
    40021 aaattatctt tttcataatc taaatctttt tcgatactct tttttctatc ctttaaatct
    40081 aaaattatac ttatgctttc atctaattta tcttttatac ctgcatctaa tggcatgtaa
    40141 tatcccccat ttatatttca aaatcttttt tcgctgaatc tatactatta atttctgacc
    40201 ttttatcact ttctactact aaagtagctt ttccaactgg ttttataatt aaatcactta
    40261 gtagtttagc aaaactcttt ttacctatag ctttctccat atcactaatt ccttttaagg
    40321 tccttggctt ataaattttt tcttcatcat aatctgaatt taataaaacc ttagcaactt
    40381 cttgctcatc tacatacttt ctattacttc tgccttctac aagcttatat cctggatatt
    40441 taacaccatg cttttcagct tgttctagtg cataactttg aacatctttt aaccaatctt
    40501 gtatattctt agcaaaacct aaaatatctg ctatctcata tttattaaga gcaaatgtgt
    40561 cagcaaaatc atattttcta gctagtttaa ggttatcctc agctcttttt ctgcaatcat
    40621 ttttagctct acaaaatcca caatgactgc cacttacaaa ctctccttca ccattaaaag
    40681 ccatttgagc cttcttctta acattatctg cccatgtaag taatttagta acttctattt
    40741 cttcacttga tatgttatca agtctaggtt gaattattgt tgttttaatt aaatcaatat
    40801 cgtatagcat ttcaaactga ttatatgccc ctaaaccata tagtctaagt tgaggattct
    40861 ctatagctga aacttctaaa ccttttccat actttaggtc tataacttga agtataccat
    40921 cagatattac aacaacgtct cctgttccaa atccttctgg aacccactca ctaaaatcga
    40981 gtctttcttc taacatcact attacatcat cacatatagc tttactatca tttactaatt
    41041 caactacatt ctcaacataa gattgtatat agtcctccat ttcagagtta taatactcac
    41101 ttttctgtat ctttttaatt cttgcattat atgccttttt acttattttt tcatactcta
    41161 gcattaattt aacttctgct aattcatgtg caactgttcc ttcttctgca tatatactag
    41221 ttgatggtgg atagttttct tctaatttta tactaggagt gcagtgaagc catctatggg
    41281 ctccactcgc actaagtctt gcatgttgta atggcattta tgtaccccct ataaactttc
    41341 taatttgttc ataaaagctg aataatcttc ttcttttact tcacttaact tactagctcc
    41401 aaattcacca aataactcct taagcttatc cttcttacct gccttactta cttgtgctgc
    41461 cttagttctt acctcttctt ttgtgtattt aacttcacta gttgtatttt cattattctt
    41521 ttctatttct tcctttgctt ctgctatttc ttctttaacg tcttctttta cttctacctt
    41581 tttaatcttt tcagcttttt taacttcaac tttcttctct tctttcatat ctgttatatt
    41641 tataggttct atctgcattg cttttcctag attaagacct cctaaagcat tcgccactac
    41701 taatagtgca tttgtaaatt ctggtgcttc tactttaact tttacattta cattcacttc
    41761 aaccatttta aatctctcct ttttgtgtta tactttactt gtgttatatt ttatttttta
    41821 tttttttgtg tgttggttat tttaccaaca cttttttatt taatattcca accgatattt
    41881 tcttaccagt tttaatatct ttaaatacta catctgctat aacttttcca tcttttttaa
    41941 gagttactac attcttatta ttagtatcaa gactcagcaa tttcatcccc cctctctact
    42001 gcttttaaaa gttcatccaa atttttacct tgatttcttt caataaaatc atcaacttca
    42061 tatcttgaaa tttttctacc atcacctcta actagtgatt ttatcaaacc tgtgcttact
    42121 aacctacgca taaaagctgt atctaacttt aaaattcccc ttgcttcttc tactgttatt
    42181 agataatttg gataacctct ttttattaaa acaactatat ctttaggctc cagtaccttt
    42241 accttttgtt caatagtttg actcttaact ctatctgtct cttgtttact tatctctatt
    42301 tctattaagc tttttaagct atcactaaat tgttttgtta tgctttcaga aatatccatg
    42361 tgaaaatgct ctcccctctt ttcaattatt tatcttccaa ccaattcgtc taatgtaata
    42421 tctaaataat cagctaattt tattagagta tctatagttg gattagaact tttatttctc
    42481 agtaatttat ataaaccact tacatctata tccaattctt ttgccaattt ataaggtttt
    42541 atatctcttt ttcttagtat ttgctttata ttgtcgccta ttaacattgt attcacctca
    42601 gttctacatg gtataatata aacattgaat aaattccaaa attttcaaaa ggatggtgta
    42661 ttatgcagtt ttcaaacaat atatctaaag aattacaaga aagtataaga aagacagtct
    42721 ctgatacttc taacacagag tatttttact atgctgaatt tcaatataaa attattctta
    42781 aatctataga ggaatttgaa aaagaacttg atgatgaaca tgaaattgca ctaaaattga
    42841 ctaactttgg aaaagatgta ttaatgattg ttgaagaagt aggttatcat aacccttgtc
    42901 taattcacta ctacggcata gtaaatggag tttattctga aattcttcaa cacacatcac
    42961 agataaactt tatgataact tcagtaaaga aaactgaccc ttcaaaacct gctagaagaa
    43021 taggttttat attgtaatta tcattcttat tttctgattc tttctttagt aatttcacta
    43081 atttttcttt tttcactttt attcatctca tgtaaataca aaactataca ttggtacata
    43141 taatctataa aatcactatt tgattgagct gttatctgac taaataactg tgcttgcaat
    43201 tgattttcaa gcatggttat tcttttctct atttcttcta ttttcttatt ttccaattca
    43261 atctctcctt tccaaaatat tctgtattta gttttcaaag tactaaccta atattgaagt
    43321 ttgataacta tattcttttt ctatatctgg taatatgtta tttgttttca ataaatcata
    43381 taaaaacaat ctaccttttt gagtccactt agttgtcatt tttacatcag gcattccatt
    43441 acttctagtt atgtctattg tttctgaatg agtgtatccc ttcccttgat gttgtttata
    43501 taaaagccat tgtccacttt gtttatattg aatccctctt tcatgaagta ttttgttcat
    43561 ttcttttcca ctcattccat aatcctttgc tatttgagtt atagtgacta gacctttgtt
    43621 tttaagtatc atatctgtat aatctgcctt tggttttagt tcttttatta ctttttcttt
    43681 cattttactt tctagttgta actgctcttt ctccttttgt tcctctatcc aacgtttagc
    43741 acgctctata ggattatcta tcatataact atcttttaat tgtcgattta ccatgttata
    43801 tgtgccagtt ttacgaatac ttggtagaac ttcatctgta acccaatctt gaaatctctc
    43861 tgcttcttct tttcttgatt taaagattaa cttatacaca ccactttcag taagaaaatt
    43921 ttctcctgta ttgtgtagtt ttctaaagtc agtactactg attttagaat taattaactt
    43981 aattacttgt ttattattca tatttctcaa attttcattt acatttttta tatctaagca
    44041 ctctgcaaca tgttttgggt taaataaaat ctgcccttca aactcaaaca cttctacttc
    44101 ttttccttca aataccatta agttattact cataaattct ttccccctta attatttata
    44161 gttgattttt ctaaaagctc atttgatact ttcataccat ctgccattgc agctatttat
    44221 tctgttttta gttttcaaag tactatcaat ctttagccta ataaagttat ttgacactct
    44281 cgttcttttt ctctaattaa tccatctttt ataagtaaca atctaaatgc ttctctacct
    44341 tttggattta ttaatgtttg agtatctgaa tgcccgtatg gtgttgtaaa ttctttcagt
    44401 tcaaagtact gcatcttatt agaataaggt tttatctttc cttttaaatc tctgtaacag
    44461 tatttcttct ctattaacca taaaacaaat actttttctt ttactccaag ttcttttgat
    44521 gtatctctta tattagttag taaatttctt tctactaaag catcaaagta atctgcctta
    44581 ggtttcattg tttgattttc tagttgtagt tgttcttttt cctcaacttc tattaataac
    44641 tgttgtaatg cttctttata tgtagttggt aatttagttt gttcattttt taaagctttt
    44701 tccatttcat caaatttttt tgtatatatt cctgtaaatg ctgtaccttt tatgcctgtc
    44761 attttatttg ctataaagtc acaacctatc tttgttattg cgtaacatgg tttctttctt
    44821 ttataatcat ctaaataagt attttcaata aaataatcag atgggtacaa atctgtactc
    44881 atgtttttat ttgcttcttc catttggttt atataatttc ttatatccct tagtaaaatc
    44941 ttgtgttctt tttcaaccat tagagctata tccctactat cagttgttag tttattatta
    45001 acttttacta tttttaaatc tttcatattt acaaactcct ctctataaaa tgtccatcat
    45061 tttattgcca ttttagcaac taattctgaa aaaaaatagt ctgttgatac tccatataat
    45121 tcagacaact tttttatttc atttgctttg aactcatttt taccattaag tttaagccta
    45181 aaaccaaata aactaagacc taacaaatct gcaacaaatt tttgcgtatg ttggttttct
    45241 ttcattaacc ctttcaatct attgacatac attttttcac cctctaactt tttgctatat
    45301 aagcaagttc ttgtttatat aatagcaaca aaaatgttaa aagtcaatat ttttgttgct
    45361 tttttggcaa ttttaattgt aataacaaaa aaagttgcta taatgtaaat aaaagttgct
    45421 ataatgaaaa ctacaataaa ggatgatttt atatgtttag attaaaagaa ctgagagaag
    45481 aaaaaggaat atctttagat agattaagta tggatttaaa tgtaaataaa tccactctat
    45541 ctaggattga aaatgggtta agagagccaa aacaatcttt tattgaagac tgttcaaatt
    45601 attttgatgt atcaacggat tacttacttg gtaaaacaga cataaaaaat cccaatcaag
    45661 tagtaaaatc tttaaatcct attttctcaa aaagattaag agaattaata aaggaaaaag
    45721 gtgtcacatt aaatatatta ggcaaaaaat tagaaataag tactgattta ttatctaaat
    45781 atgaaaataa tataatttca cctaaaagtg atgatattat aaagcttgct cattattttg
    45841 atgttacaac agattatctt ttaggtacaa cacttgttag aaatcatata gacactgtag
    45901 ccgcacacaa agcaaatcca catgaggatt tacctgaaga agctcaagaa cagcttaatg
    45961 attatattga atttttaatg aataagtata aaaaataaat taaaagtagg tgataaatta
    46021 tatgaaatct ataatatcag atgtcataaa ccgatttaat gctacaccat ttttatttgt
    46081 tggttctggt ttaactagaa gatactataa tcttccaaat tgggaggatt tactaaaagt
    46141 ctttgcagaa aaaattagta atgatgactt tatatataca agttataaaa ataaagcaaa
    46201 atcagagaac ccaaaaatgg gtataaatcc tagaattgca gaacttattg aaaatgattt
    46261 taataaaaaa tggttttcag attcttctat acgaagctta gactctaagt atttagatat
    46321 tgttaaatca ggagtttctc catttaaggc agaaattgct atgtatatta aaaataattc
    46381 taaaatagta gataaatatc aagatgaagt tgaaaaactt agtaatatct ctaaaaaaag
    46441 tttatcagga tttattacaa caaattatga ttgttttttg gaaactatcg ttgataatta
    46501 tacaacatat ataggtcaag aaaatttagt tttttcatct atacaaggta ttgcagaaat
    46561 ttataaaatt catggatgtg tttcttctcc aaatagtatt gtaataaatg aagctgatta
    46621 tatagacttt gacagcaaaa gtgcctactt ggcagctaag ctaatgacta tatttgttga
    46681 gtttcctatc atatttattg gttactctgt tacagatgta aatataaaaa aaatacttaa
    46741 tgctatagtt aattgtctat ctaatgaaaa tgctaaaaaa ctcgaggaac gtttcatatt
    46801 cattgagtat gaaaaaaact ttaatgatat agaaatatca agtcatacaa ttgcttttga
    46861 taataaaatg attactatga ctaaaattaa actggaggat tttaatttac tctatgaggc
    46921 attatctgaa aaaaaatcaa aattaccagt tagaatatta agaatgttca aacaagaatt
    46981 ttacgatttc acaattacta acaaacctac tgctaaaatt cgggttggta gtgttgatga
    47041 tacaagaata aaagatgaag atttagtttt agcagttggc aaggcaagtg attttggtct
    47101 taaaggatta aaaggattaa gttttgatga atggtatcgt gatatagtca tgaatgactt
    47161 agaattttca tcagatgaat tacttgaata tgcttatcca tctttaatta agcaatataa
    47221 taaattacca ttaaataaac atctttttaa tagcacactt gaatttccag attataggac
    47281 aattgcacta gaaagtgact ttgaaaatat tattagcaat agtattaaaa aaaatagaaa
    47341 taatacttac ataaaaaata gaagtgttta tggtatttgg aatgatgaac atcaatcatt
    47401 tgaaaaagct acaagactta ttgctttttt agaagaaaaa gaaattgaca ctgaacagtt
    47461 agaaaatcta cttaagaaaa tatttgagga aaatcctaat attttagagt ctgcaaaaac
    47521 aagcgaaaaa acaaatttaa gaaggcttat tagaatatat gactacatga aatactctaa
    47581 tatgcaaaag agtcttggtt atagtgagta aaactctacc caccgctcca aaactccttt
    47641 aatcgcttta agcacaaata caaatttagt gctaactata ctaacacaca tttgtaagtg
    47701 cttacatata ttatatgtaa ttatattata atcatatact ataatccata ttttgtctac
    47761 agctaaaatt atccaatctt ttttggataa aaaatgaata tttagagcag ttcacacctg
    47821 ctctttatat ataacaaaaa tcaaaaatac attcttttta tagggggatt tcaatgaaca
    47881 aactagacgc acttttagac ttagcaaata atgaagagat agaaatttac tacactgaca
    47941 aaatagcaga tgacataaaa ggattgtata taaacagaca aggactaaag attatatcat
    48001 tacttaattc attaaaacaa aacaatgcta aactaataga aatcttagca gaagaattag
    48061 gacatcattt taccagtgtt gggaactatg tatcttcaaa aaacagttac aaaaataaaa
    48121 tcttgataga caaaactgaa aacaaagcat taaaatgggc atgtgaattt cttataacag
    48181 aagaagaaat aatacatgtt attaattcac acgctacaag tgtatacgaa atagctgaag
    48241 aattacaagt tagcatcaac ttcttactaa aaagattaga atttctatca aaaaagaaaa
    48301 gcatgttgga cttaggaaat aatagatttt tagtattaac taatttgcca aatttctaca
    48361 tatatgagga tattttttaa actcatttat tctactttta tagatttttt acttaataaa
    48421 tatatatttc aatattatta taataaacta cacataaaag ctaaaaaatt gtaagaatat
    48481 taagaaaatg attaagtgaa aaccagataa acaaaattaa gataatattg tacataacaa
    48541 aagtatataa agagcagtta atctgctctt ttatataaac accaaacaaa catacattct
    48601 aaaagggagg gatactatta tgaaaggtgg agtaagaaaa agaagtaaca aatggtatta
    48661 ctactttgac ctaggcatag tagaaggaaa aagaaaaaaa gtagaaagag ttggaggcaa
    48721 tactaaaaaa gaagcagaaa aagccttaag agaagcacta aatgaatatg aaaactctgg
    48781 catagtattt gaagaaagca atatcagttt atcagactac ttagactttt ggtacaaaga
    48841 atatgtctta cttaactgta aatacaacac tcaagaaagc taccgaataa acatagaaaa
    48901 acatataaag ccaaagctag gagcttacaa agtaaaagct ttaactcctg caatactaca
    48961 aaacttcata aacagaaagt acaaagagga ttactctcaa aatacattac aagtattaaa
    49021 agccatatta cataggtcat taaaatcagc agtccatcct tacaaacaca tacgagaaaa
    49081 ccctatgcaa tatgtaagca taccaaaaac taaatctaaa acagaaacta ataaagttaa
    49141 aactattaca ttagaagaat ttaatcaaat actaaatata tttcctcaag attcatttca
    49201 acgtatagtt ttactaattg gatttcatac tggtatgcga agaggtgaaa ttattgcact
    49261 aaaatgggat aatatagacc ttgataataa aactatcaca gtaaagcata ctttgattaa
    49321 aaaaccaaat ggaatgtttg aattagggca accaaaaaca gaaagctctt gcagaactat
    49381 atttacaggt gacactttaa taaaggcatt aaaagaacat aaattatatc aaaagaaaat
    49441 gaaattaaaa tacggagaat tttactttga tagtgattgg gtatgtacca aagaaaatgg
    49501 tcaacaagtg aatactcaca ctttagacac tatagtaaga caaattcgag tagctttaaa
    49561 caatgatttc cattttcatt ctttaagaca tgcacatgct actctattat tagaaaatgg
    49621 tgctaacatt aaagacatac aaaaccgttt gggtcatagc caactatcaa ctacaatgga
    49681 tacctattca catgtaactg ataaaatgaa aaatgaaact gtagatatat ttgaaaaaat
    49741 tacaaattag agtttgccac ccaaaaatat aatacggtgg caaatgggtg gcaaaatcta
    49801 atttatctat tttaaaagct aaaattatca aatttatata gtcagctata cacttgtaat
    49861 ttcaaggctt tagagtatat aacaaccata actaatataa ggtattaata ataaatctaa
    49921 cgaataaaac ttaatattta cttaagttgt aatattattc taaattgtat aattaaaatt
    49981 taataagttc ttaattatat agtcattgat ttattcgcaa aaataaaaaa tgccactcta
    50041 tctctaaaca aaaactgaat accaataaaa atttaataaa aaattttatc aatattcagt
    50101 ataagtttta gttacatatc aaattttcca atgcatcatt aggtgctaca ataggataaa
    50161 catcataatc cttatctata tcacattgta aaaatacagg tttattaaaa tctatcatat
    50221 ctatggtctt tcctaattct tccatattgg atactttata tccatcaatg ttatatgcat
    50281 ttacgagttt aacataatcc acattttcat taatgtcagt ttgagagtat ctttgattgg
    50341 aaaataactt ctgccattgt cttaccattc ctaatgttct attattgaat agtaaaataa
    50401 gcataggtac attataattt gcaactgtag ctagttcatt acagttcatt ctaaaacttc
    50461 catcaccagt gactaaaacc acatttttat ctacattacc agctttagtt ccaatagctg
    50521 cacctaagcc aaatcccata gttcctaacc ctgctgatgt tataaaactt ttattacctt
    50581 taaaattcca atacttagca gcccacattt gatgttgacc aacatcagtt actaccacag
    50641 tgggcttctt taatgtttca tatttctcat ttattttttt aagtatattt tgtggatgaa
    50701 attcatctgt ttgtataccc tcactctttc taaatgtttt tatttcttct ttccagttac
    50761 tattattctt actttcaacc atttcaagta ataaactaag gactaacttt acatctccaa
    50821 ccaaagatac attagattct atatttttgc ttatttcaga tgggtctata tctatgtgta
    50881 ttatttttgc atttttagca aattcagagc ttttacttat cactctatca ctaaatctcg
    50941 cacctatcgc tatcactaaa tctgaattag aaagtgctaa attactttct ctacttccat
    51001 gcattccaac cattccaaga gatagttcat tttttctatc tatattgcca agccccataa
    51061 gtgtatttag tacgggtgta tctatcttag ttgcaaattt ttcaagaatt tcctcactgt
    51121 ctgaagattt aacacctcca cctgcatata ttacaggctt tttagattct tttatgatat
    51181 ctattgcttc atttaaaagc ttagtattag tttcatcatc tagaccaaaa tcactcttat
    51241 aatccatata atcatctatt tgacataaat catagtcttc accactaaaa tccatttctg
    51301 ctaaaaataa atcttttgga acatctacaa gtacaggtcc ttttctgcct gaatttgcaa
    51361 ctctaaaagc ttcttttatt gtaggaacta attctttagt gtttctaact aaataattat
    51421 gttttgtcat agataatgtt gctccagtta tgtcaatttc ttgaaaagaa tcttttccta
    51481 ataaactagt tggcacctgc cctgaaatta caagcatagg tgaagaatcc ataaaagctg
    51541 ttgctattcc agttatggca tttgttgctc ctggaccaga tgttgtaaaa caaactccta
    51601 ctgtattagt acttcttgcg tacccatctg cagcatgaac taaaccttgt tcatgggatg
    51661 tccttatatg tttaaaatca tctgaataat cgtatagagc atcataaaga ggtattacag
    51721 ctccaccagg atatccaaat attgtgtcaa ttccctcttt ctttaaacat tctaaaatta
    51781 cctttgctcc attcatacgc actactatac accactcttt ccctcaaact aatccattta
    51841 atatatattt attttaagaa tgacatcata cttcttagac ttctaccaac ttcggcaatt
    51901 tctgtattat attcttcttc tctaattttt aggaagtttt cacgtccttc ttcattctct
    51961 ttaatccacg cttttgcaaa ttttccattt tgtatatcct ctaaaacatt ttgcatacct
    52021 tgttttgcag cgtcggttat taccctttta ccagatacat aatctccata ttctgctgtg
    52081 tcacttatgc tatatctcat tctttcaaat ccaccttcat atataaggtc gactattagt
    52141 ttcatttcat gtaaacattc aaagtatgct acttcttttt gataacctgc atctacaagc
    52201 gttttatatc ccaattttat aagttcactt attccaccac aaagtactga ttgttctcca
    52261 aataaatctg tttctgtttc ttctttaaaa gtagtttcta aaacgcctgc tcttgttgct
    52321 cctatccctt ttgcatatgc taaaactgtc tctgtagctt ttttagtgta gtcttgatat
    52381 actgcaaata gtgcaggaac tccacttccc tttgtaaata catttctaac taagtgacca
    52441 ggtcctttag gagctaccat tacaacatca acaaattctg gtggttgtac ttgattataa
    52501 tgtatattga atccatgagc aaaaactagt gtctgacctt cttttaagtt atcctttaca
    52561 ctctcttcat aaactttctt ttgtttttca tcaggcatta acagcattgt caaatcactt
    52621 tctttcgtag cttctgctac actcttaact tcaaatccat cttcttttgc tttttgtgct
    52681 gatttacttc cgtcataaag acctatcact acatgtacac cattatctct aagattttgt
    52741 gcatgtgcat gtccttgact tccataacct aaaactgcaa cctttttatt ttttaaaacc
    52801 tctaaatcca catctttttc ataatacatt cttgccattt tcatttcctc ccaatacttt
    52861 ttcttatttt tatgatattt atttttatta gaaattcata ctcattatca ttgttttaat
    52921 attattcaac agatttaaat tctaaattta tataatctta caattataat tttagaattt
    52981 tcagttttta ttagctacga caaactaata aatttattaa ctgtgataaa ctgataaatt
    53041 caatgacatt tgtattttag tctaagattt ttcttattta atatttatct ttagtttttt
    53101 attttaaaat aagcatattt atctttagct ttataaaata tatttaatta atgtctcatt
    53161 ctttcaacag ttatctctct tatatcatgt agttttttca tatggtttaa gacagaatct
    53221 aaagatgtct tttcctcatt tactat
//
LOCUS       DAEMAE010000006        49901 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEMAE010000006 DAEMAE010000000
VERSION     DAEMAE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.006"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1285
                     /locus_tag="KPH93_000303"
     CDS             <1..1285
                     /locus_tag="KPH93_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1687144.1"
                     /translation="DRIKKNNYIESVEVVNYIDNNIDINIAKDISNFLESEYNITLTS
                     AELYYLVFQLTNKTTVLNYNQMDTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEE
                     FVVKFTLHVKNLISRAKNNQVLRNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDE
                     DEISYISLHVGSFFDRQKLLEYKVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIF
                     SDTHNLDFDNKVDMVITTLPISDRCPIPFVYVNLYLNRKDYDNIQSKFIQIKDRKNIL
                     TVQNHLEMYFSESLFMKNIYLDSAKDYIKFMGNILCKNKYVKPNYIDDVLIREKMSST
                     AFNNNVAIPHSMKMDALKTGVCLIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFE
                     SFIEILSEWNNIKELTKADNYSSFMNRIAYLIQNI"
     gene            complement(1408..2562)
                     /locus_tag="KPH93_000304"
     CDS             complement(1408..2562)
                     /locus_tag="KPH93_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG1687145.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDVQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKDTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGVGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGTGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(2844..5459)
                     /gene="mngB"
                     /locus_tag="KPH93_000305"
     CDS             complement(2844..5459)
                     /gene="mngB"
                     /locus_tag="KPH93_000305"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG1687146.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERVKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIYKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLIIFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDRDNNKLDFEVVHKEEIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEYMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANHTIKKNRFNNEININYRLK
                     VPKDIQARKNNREDSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMVRRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVIKKAEKEEALVVRFYNSKEETKSANIVFNKNIKIAKWINLNEKELEFISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(5602..7527)
                     /gene="mngA"
                     /locus_tag="KPH93_000306"
     CDS             complement(5602..7527)
                     /gene="mngA"
                     /locus_tag="KPH93_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG1687147.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFEKGLAIPHGKSKSVKEAAFAAMTLEIPIEDWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNVKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQDL
                     YNQEGSWLWQLRKLSSGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATILGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFMAASIWFFAAVLGAIIS
                     AMLLIITRKQKNQRKIY"
     gene            complement(7657..8382)
                     /locus_tag="KPH93_000307"
     CDS             complement(7657..8382)
                     /locus_tag="KPH93_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1687148.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNKNKESKYGFESDIKKTLELIDYEKIN
                     KVADIIQKSRNIIVYGIGQNIPLCEILVKEIRGSHNNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKIAVDKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(8645..9424)
                     /locus_tag="KPH93_000308"
     CDS             complement(8645..9424)
                     /locus_tag="KPH93_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG1687149.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDACFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMNKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKEITLMVTHPGFIDYSLIKSSS
                     LVQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKANNYI"
     gene            complement(9417..10844)
                     /locus_tag="KPH93_000309"
     CDS             complement(9417..10844)
                     /locus_tag="KPH93_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1687150.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNGDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNRTYKVKVPAFLSFFAGEKCVITLSPVVALILSYVFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPIGIVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            11235..11957
                     /locus_tag="KPH93_000310"
     CDS             11235..11957
                     /locus_tag="KPH93_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1687151.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNAEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKNLDVSIA
                     QGSIYKYIEESLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            12153..13820
                     /locus_tag="KPH93_000311"
     CDS             12153..13820
                     /locus_tag="KPH93_000311"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG1687152.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLSSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKKQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENEMEIKPYESIMIYYK"
     gene            complement(13916..15235)
                     /locus_tag="KPH93_000312"
     CDS             complement(13916..15235)
                     /locus_tag="KPH93_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1687153.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFIGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPRSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(15276..16022)
                     /locus_tag="KPH93_000313"
     CDS             complement(15276..16022)
                     /locus_tag="KPH93_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG1687154.1"
                     /translation="MKKVVKKKYPVIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CEAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(16266..18275)
                     /locus_tag="KPH93_000314"
     CDS             complement(16266..18275)
                     /locus_tag="KPH93_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1687155.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVIIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKVAITPGEASLVPKYIKEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLSDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINTYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEIRILNDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNLINE
                     LGYSITEGEVRGILNILKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(18713..20146)
                     /locus_tag="KPH93_000315"
     CDS             complement(18713..20146)
                     /locus_tag="KPH93_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG1687156.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDRPQLRFQGLHHQFV
                     ASAKAVKLAHETDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(20233..22149)
                     /locus_tag="KPH93_000316"
     CDS             complement(20233..22149)
                     /locus_tag="KPH93_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687157.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYAVVCDLAGLGGDTVDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFSALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAIISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVIGGLGIFGIPNYINPTTGDMTGVYATIIA
                     IIVAIVVGFVLTLFLWKDDAIVEISKDDNGQGVVEKNKREVIYSPIRGYVKVLEQMQD
                     AAFSTGVLGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHVGLNTV
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KNVNNSQELITVLA"
     gene            complement(22223..23083)
                     /locus_tag="KPH93_000317"
     CDS             complement(22223..23083)
                     /locus_tag="KPH93_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1687158.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGDHISKDKI
                     DKIFKLSDPNISDKFKELIADIPMRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVNVKNVLLWDIKRFYKNEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNVHMDQSIKIIYEITQIIQEIANVVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHESNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(23434..24621)
                     /locus_tag="KPH93_000318"
     CDS             complement(23434..24621)
                     /locus_tag="KPH93_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1687159.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTTYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEVGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSGEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(24635..26026)
                     /locus_tag="KPH93_000319"
     CDS             complement(24635..26026)
                     /locus_tag="KPH93_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG1687160.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDSNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVVPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(26622..28322)
                     /locus_tag="KPH93_000320"
     CDS             complement(26622..28322)
                     /locus_tag="KPH93_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1687161.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(28294..29697)
                     /locus_tag="KPH93_000321"
     CDS             complement(28294..29697)
                     /locus_tag="KPH93_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1687162.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDLDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(29962..30453)
                     /locus_tag="KPH93_000322"
     CDS             complement(29962..30453)
                     /locus_tag="KPH93_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687163.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFIMSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(30479..31291)
                     /locus_tag="KPH93_000323"
     CDS             complement(30479..31291)
                     /locus_tag="KPH93_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1687164.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENVKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGQEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(31304..32455)
                     /locus_tag="KPH93_000324"
     CDS             complement(31304..32455)
                     /locus_tag="KPH93_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1687165.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPDRITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANSGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIKKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            32725..33366
                     /locus_tag="KPH93_000325"
     CDS             32725..33366
                     /locus_tag="KPH93_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG1687166.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFYEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            33392..34936
                     /locus_tag="KPH93_000326"
     CDS             33392..34936
                     /locus_tag="KPH93_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1687167.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDVKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(35119..35745)
                     /locus_tag="KPH93_000327"
     CDS             complement(35119..35745)
                     /locus_tag="KPH93_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1687168.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNVNSACAQ"
     gene            complement(35880..36596)
                     /locus_tag="KPH93_000328"
     CDS             complement(35880..36596)
                     /locus_tag="KPH93_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG1687169.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAIDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            37022..38236
                     /locus_tag="KPH93_000329"
     CDS             37022..38236
                     /locus_tag="KPH93_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG1687170.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            38856..40184
                     /locus_tag="KPH93_000330"
     CDS             38856..40184
                     /locus_tag="KPH93_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687171.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIT
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYIKVKGTNYDDSILEQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTTCYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(40312..41502)
                     /locus_tag="KPH93_000331"
     CDS             complement(40312..41502)
                     /locus_tag="KPH93_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1687172.1"
                     /translation="MKKFFKEAQSIEEEIIRWRRYLHENPETGCNLPRTVTFVTSMLE
                     EMGYSPELTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKGASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGVLENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGIAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSVNQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(41608..42987)
                     /locus_tag="KPH93_000332"
     CDS             complement(41608..42987)
                     /locus_tag="KPH93_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1687173.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGVFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTQDELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKRAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(43018..44226)
                     /locus_tag="KPH93_000333"
     CDS             complement(43018..44226)
                     /locus_tag="KPH93_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687174.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(45251..46705)
                     /locus_tag="KPH93_000334"
     CDS             complement(45251..46705)
                     /locus_tag="KPH93_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG1687175.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNINIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDSSTDIVVE"
     gene            complement(46725..48596)
                     /locus_tag="KPH93_000335"
     CDS             complement(46725..48596)
                     /locus_tag="KPH93_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687176.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSVKGEGKKEIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQVTTYANSFIPI
                     LLGVWSMGIIIGGLKKILPKALHYFLVPVLSLIITLSVTLTIFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIIGVVVSFAAATVASYLLGIP
                     SDEKEEVKDDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESFV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(48586..49446)
                     /locus_tag="KPH93_000336"
     CDS             complement(48586..49446)
                     /locus_tag="KPH93_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1687177.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RIYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEVGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT        16443 a         8819 c         5649 g        18990 t
ORIGIN      
        1 agatagaata aaaaagaata attacataga gtctgttgaa gtagtaaact acatagacaa
       61 taacatagat ataaatattg caaaagacat atcaaatttt ttagaatctg agtacaatat
      121 aactttaact agtgcagaat tatactactt agtatttcag ttaacaaaca aaacaacagt
      181 cttaaactac aatcaaatgg atactaagtc attgtcaaat tatatagatg aacactttgt
      241 taaattaact aaaaaaataa ttaaaaatgt atatgactta tatcttatag atttatctga
      301 tgaggaattt gttgtaaaat ttactcttca tgtaaaaaat ttaatcagta gggccaaaaa
      361 taatcaagtt ttaagaaatc aaatacccca aaagttaaaa gattcctacc ctttgatata
      421 tgatatatct gtttatattt gcaatcagat tcaaacttta gaaaacgtag atatagacga
      481 agatgaaata tcgtacatat ctcttcatgt aggttcattc tttgatagac aaaaattatt
      541 agaatataaa gttctatgtg cattaattac tccaaattac tatgatttac aattcaaaat
      601 agtaagagac cttgagaaga gatttaatga atccatagaa attattcaga ttttttcaga
      661 tactcataat ctggattttg acaataaggt agacatggtt attactactt taccaattag
      721 cgatagatgc cctatacctt ttgtttatgt aaatctttac ctaaacagaa aagattatga
      781 taatatccaa agtaaattta ttcagattaa agatagaaaa aatatactta ctgtacaaaa
      841 tcatttagaa atgtatttta gtgaatcttt gtttatgaaa aacatatatt tagatagtgc
      901 taaagattat ataaaattta tgggaaatat tctgtgcaaa aataaatatg tcaaacccaa
      961 ttatatagat gatgttctta taagagaaaa aatgtcttca actgcattta ataacaatgt
     1021 agcaattcct catagtatga aaatggatgc tttaaaaact ggagtatgtt taattgtcaa
     1081 cgataaacca gttaaatggg gagaggaaaa agttcagatt atagctatga tacctataaa
     1141 tgaaaaggaa aaagaaaaat tcaattacat atttgaaagc tttattgaaa tattatctga
     1201 gtggaataat attaaagaac ttactaaagc agataactat agtagtttta tgaatagaat
     1261 agcttatctt attcaaaata tctaaatcca ttaatttgtt tccaatatat gtttcttaag
     1321 ttattaacac aaaatagaca agctgtcaca ctaaaaagtc taaattaata tgtgacagct
     1381 ttttacctaa tataccttgt tttaatacta catatttttt gactgttgta tactagttat
     1441 aagattgaat atttgtgttg ttgtagctgt catattctca ctagcagttg ctttatccat
     1501 tgcttcttct attgtcatag ctctatttac tatagaaaaa taagcatcta taccttcttc
     1561 attacattta actgcatctt ctgttgtggc tccagccaaa cctattactt ttaccccata
     1621 tttttttgcc agttttgcaa ctcctattgg agccttgccc atagcagttt gattgtccaa
     1681 tctaccttca ccagttatga caaaatctgc atcttttaac tcttcctcta atttaatttc
     1741 ttctaatatt atttttattc cagattctaa ttttgaattt aaaaatgcta aaaaagcaaa
     1801 acctaatcct cctgctgctc ctgttccttc tacattagct acatctttac ttaagtaatt
     1861 tttaactact ttagaaaaat ttctaagccc attatctaac tcctcaatta tttctgatgt
     1921 tgcacctttt tgagacccat atatgtatgc agctccattt tttccaaaca gtggattatt
     1981 tacatcacag gctattttaa acttacattc atctaattcc tttaatctat tttctatttt
     2041 tatatgtctt atttcattta aaacttttcc acctaatcct actaacttgt tattttcatc
     2101 ataaaactca aatcctaagg cttgaagcat acctacccca gcatcattag tagcacttcc
     2161 acctattcca actataaagt ttctatatcc tttatttatt gcttccttta ttatttcccc
     2221 aacaccataa gttgtagtgt ataaaggatt tctaagttca gtcggaacta gtgttaatcc
     2281 tgatgcttga gctatttcta ttattgctgt acttgtatct tttaataatc catatgttgc
     2341 atccactttt ttagctattg gtcctgtaac ttctatagtc tctttcttac cattcattcc
     2401 ttgaactaaa gcatctactg tcccttcacc accatcagca agtggcaata tctttacttg
     2461 cacatcttct ttaaccttca atatcccctt ttttattgca tttcctgcct ctattgaaga
     2521 taagcttcct ttcaaagaat ctatagaaat taatattttc acatttatct actccttttt
     2581 atttactcct tattttaaac atataatttt ataagcttaa tttatctttt attttatact
     2641 ctacatctct ctttgtatac aacttatacc attaatattc ttttatctta taaaaaaggg
     2701 agtgtctcaa aataaactat ttagttcatg agacactctt tttgtataaa ctcaaatata
     2761 tgcttaccat ttcaacaatg ataaggagga ttatcgctat tttaagacaa ttacttttta
     2821 gcactatcta atcattttta gcactattta accaatatag ttttaacttg gttcattaag
     2881 cactctacat ttacagagtt attatctatt gatataaatt ctaattcttt ctcatttagg
     2941 ttaatccatt tagctatttt aatgtttttg ttaaatacta tgttggctga ttttgtttct
     3001 tctttagaat tataaaatct aactactaat gcctcttctt tttcagcctt ttttataaca
     3061 cttaacacaa ggtccttatt agtttcctta agcaaactat atgaataatc tacttctatt
     3121 tctgattgat tcaatttcat agcattatat ggcattttat tataagtttg tatttttgtt
     3181 aaatactctt tagccatatt tgcaacattt gcttctagtg tagatttttc atgagtaact
     3241 attgctaaat ctatttttat atctcctatc atttgagaat ctggagtagg caactttatt
     3301 cctgatggac gacctgggcg tcttaccatc tcttctttac ccaggaatcc tacacttcta
     3361 aataatgtta tggctattgt gtcaaatttt tctcctacta tttcaaactc tctcgtactg
     3421 tttgtaagaa cagctactcc atgctctttg tcagacaatc caacgaatgt tagcattgga
     3481 tatatagaat ctggtctttc actccattct tcctgttgcc aaacttccat agcatcatca
     3541 tacacacttc tctttatatg tccaaactga ttatcagaca cagaaaactt tgaagctata
     3601 tttgttggta tataagttct aaccctacaa tctcttgctt ggttatttat gttgaatact
     3661 acatctatta tagctttatc atttggaaca tttataacta tatcaaagtc aataaatgaa
     3721 tcttctctat tattttttct agcttgtata tcttttggta cttttaatct ataatttata
     3781 tttatctcat tattaaatct gtttttcttt atagtgtgat ttgctactat attatcgtta
     3841 aatattaatt tctcatttgg aagtggtgaa aaatcatact catctccttc atctcctcca
     3901 ttttccatca ataatacatt ttcaaaagtt ttatttaatt ttttatcaaa tatctttaat
     3961 gtcccgtttt cattaacttg aatagtataa aagtcattat ctaccatgtc acatacttca
     4021 taagcttttt ctaacatata ttcaacttct tttatcatat acgccttata ccccatggca
     4081 ggtattttat ctttaaagtt tattgtatat accataaatg aatcataatt tccataatga
     4141 acaatttgac ggtctattaa tcctgcatct atttcttctt tatgtaccac ttcaaaatcc
     4201 aacttattat tatctctatc aaatatttca aatgctttca ttttagttat aacttgggcg
     4261 cttactattt cattcctatc gtaaggcatt aagttaaatg ctactaactt atctaaagcc
     4321 atttcacaac tcattgcatc aactattctt cttttataaa atattattaa ttggtctact
     4381 ttttcctcag ctaagaaaaa tctattcatt atttcctggt gaactttatc tgaacaacaa
     4441 catccaatac tgtcatgagc atgatttttc attatttctt tccaaattaa ctctattaat
     4501 ccatgatgat attcaaatcc tagagaatat gcaatagaag ctaatggctc caatatgtta
     4561 gttatctttg attctattct agcatttgct gattttatat ccattcttgt tgagaatata
     4621 cttctatgaa ccctcatata tttcccatct aaaaattccc cttttattgt atctatatcc
     4681 tcattttttt ctagctcttt atatatgttt tcatatctac ttaagaaaaa ttttctctct
     4741 ggataacatt cttttaattt atatataact tcaaatatat tcttttgaat tggcatttgg
     4801 tcatgcccat ttggaagtat tatatggtca gtagttgcac ccttatcaag aactggtaaa
     4861 tatttatcca ttcttgttct tagctcatct tcattcattg gtagatattt tcctattgca
     4921 taacctaatg gaagtatttg aaccaaaaca gaagacccat catcactctt ccaattaaac
     4981 tctgttttat tagttccttt tctttcagaa catcctctcc aaaacattga gtattttata
     5041 tcaaacccat ttagtatttg tggcatttga gcactttgac caaatgagtc tggcaaatat
     5101 cctattttca tatattctcc aaattcatca caatctttta ttccatacag taaatttcta
     5161 agtatcgatt ctccaccaac taccatttca tcagtttgag tataccaagg acctattatt
     5221 agccttcctt cttgaactaa tttttttact ctctctttac attcaggctt aactgctaaa
     5281 taatcttcta atatagcagt ttgaccatcc atcacataat atggatagtt atgattattt
     5341 tctaacatat ccattatttc ttccatatta tttacaagta atattctaga ttcctcagtt
     5401 gagaagtacc attctctatc ccaatgcata tgaggaacta tgtgtacatt ttttttcatt
     5461 gcttatcctc caaaacttat ctgataacat tttctgtcta attaaaaatc caataaaaat
     5521 actactgagt ttacacaatt ttttaatttt tattttataa tataatcaaa tctttgattt
     5581 aaatctctaa taatgtatct tctaatatat ctttctttga tttttttgtt tcctagttat
     5641 tattaataac attgctgata ttatagctcc aagtactgct gcaaagaacc atattgatgc
     5701 tgccataaat cctgacatac ttccctctaa aagtgccaat gagaatatac cagctccagg
     5761 tacttgaagt cctatattga aaaatgctac tatagaccca gttatggctg aaccagctac
     5821 caatgatggt ataacacgaa ttgggtcctc tatcataaat ggtatagcac cttcagttat
     5881 tccagccaat cctaatatcc aagtttgctt tcctatctcc ttttctgatt ctgaaaataa
     5941 atctctgcct aaaatagtag ctattgtaac tccaaaagct gaaaccatct taactgatgc
     6001 aaatattgca taaggtataa aattcccact tgctattgct cctatcgcaa atgtataggc
     6061 tgctttattt attggtccac ctaggtcaaa cgaaaccata gctcctatta ttgcacctag
     6121 tagtattgca tttactccag tcattccatt tagccaattt ataagaccat tgttaagtgc
     6181 tgctacaggc tttcctacta caaataacat caataaacct actactaaag aaccaactac
     6241 tggatatacc caaaaactta cgaatccagc taatgtacct ttggctggta tattcttctt
     6301 taaaaatttt actatataac cagcaagtaa acctcctaac atacctccaa gaaatccact
     6361 gcctattaaa tcagctgcta gacctgctgc aaatcctggt ccaagacctg gtttttcagc
     6421 tagtgaataa gacatatatg ctgataatac tggaaccatc aatgtaccta acataccact
     6481 agaaagcttt cttaattgcc ataaccaaga accttcttga ttgtataggt cttgtagacc
     6541 aaatgcctga gatgctaata ctgcaaatgc aagtatcata cctcctgcca caataagtgg
     6601 tattatatga gatactcctg taagtattgc tgacttcaca tcttttttcc atgatgattt
     6661 ttcacaatat gatatatcag atgatttttc tatataatca cctttacctt gtttttgtgc
     6721 tacttctaaa gcttcgtata ttatttcttt tgcccttttt agtggagctg ctactgacgt
     6781 ttttacgttt ggcaaatgtt caaatctttc tgtattttta ggtgctacat ctgttgcata
     6841 tattattgca tctgcccttt ttattatatc tggtgtatga gggtctacca taccattagc
     6901 tccttgcttt tctacataca catccacccc tatttcttta ccagctttaa ttaatgcttc
     6961 tgctgacata tatgtatgtg ctatacctgc agggcatgct gttattgcta atacaatttt
     7021 ttttgattca ttttgaatcc taatttcttc ttttatttgt tctttctttt cttttatatc
     7081 taagttatta aataactctt tcttagtttt tgaatttaac aacatattca tataatcttc
     7141 atttgacagt ctagtaacta actcagaaag tatttgtatg tgtgtatttc cgccttcact
     7201 ttctggtact gctattaaaa atattagctc tactttatta ttggggtcta cgctttccca
     7261 atcctctatt ggtatctcta aggtcatagc tgcaaatgca gcttctttaa ctgacttaga
     7321 ttttccatga ggaatcgcaa gccctttttc aaaacctgtt ggagataaag tttctctgta
     7381 cattacagct tctaaatatt cctcataaga tgttaacttg ccttcatcat atagctttct
     7441 tgctaagaac tttattgcgt cttcctttgt ttttattttt tgttttaaaa aaacaaggtt
     7501 ttcatttgtg attttcttaa attccaaaat tacgccctcc ttaaatcatt taatgttatg
     7561 aatatagcat aaaataaatc atgaaaatgt ttttttgaaa ttatacacac aatttcttct
     7621 taaaaaataa agatttgtgt atttatacac aaatctctat cttaaccagt attgttctat
     7681 tattgctctc ataatcatca taattggaac cttatctgat atattgtacc catttacttg
     7741 tttaattgtt gaatagcaat ataaacttat atctgcaatt tgttgcaatt gattttcaca
     7801 tatatgtgtt agagctacta ccttacatcc cttatcaact gctatctttg ctgcttctat
     7861 cacttctttc gtttctccac ttaaactaag ggcaaaaaac atatcttgtt cacctaattt
     7921 tcctatatca tgaagtactt catgtctttg actatagtaa gttatattat tatggctacc
     7981 tctaatttct tttactaata tctcacataa aggaatattt tgacctattc cataaactat
     8041 aatattccta gatttttgaa ttatatctgc tactttattt attttttcat aatctattag
     8101 ctctaaggtc ttttttatat ctgattcaaa accatatttt gactctttat tcttattttt
     8161 taactcttct tttaaattat tttttagttg agagaaacca tcatactcca tttttttaca
     8221 tagcctaact atggtatttg gcacagtaaa aaatttatct gctaaagact gtattgataa
     8281 ttttactatt tcttctttgt tttttctcat atattcaatt atcatgtctt ctgtatctgt
     8341 aaacttatac tcatattttt ttgctctttc ataaaattcc atagtttctc ctccatctat
     8401 ttcattattt ataagtaaaa ttcaaaaata ctatctaaaa ttttcgttaa tactattata
     8461 atttaatact tttaactttt taatgcatac tactcctttt attatacata aaaaatgtgt
     8521 acttcctata tcaatagaga tgattcaagt ttttattttg atacatctcc atcaatattc
     8581 ttttagaaaa tacacatttt tttcaaatac ttatttttaa ttaacacttt atgtaatcta
     8641 ttctttatat ataattatta gccttgaaaa tatctttaaa atcaaccaac tcaatattat
     8701 ttctttttaa ccacatttta acttccttat cacataacat tgcatgttca tatactctct
     8761 cttgaacaag agaggatgat tttataagac tataatctat aaatcctgga tgggtaacca
     8821 tcaatgttat ctctttatct aaatttatat atttttcttc aaataattta acaaaagatt
     8881 tatttaactt gtaaaaatca ttctgaggat atttacttgg catactaata catgaatcta
     8941 aataataggg acaacttggt ataccgtatt ctttcgcagc atcacacatt gctgatattg
     9001 atacatcttc ttctatacca tgtatatcaa tatactcagg caacttattc atatacaact
     9061 tgtaagtttc aatttgagca caaacttctt catatacttc ttcataatta aaaacctctt
     9121 tacctgtcag tagttgcgtt cttcttattt tgctagaaac aaattttcca ccttcatcta
     9181 acaaattctt tatcttaata tgttctttgc aacaaggttc tcctattact aaattaacat
     9241 gtaaaccaaa acatgcatca tcatttttta ccatttctat agcttgtctt gaaaagggca
     9301 tattcaccat gattccagca cttgtaacaa taccgttctt ataagaagat attatcccta
     9361 gtgtaacacc ttctgagtat cctatatcat ctgccctaac tactaatttc ttcattttat
     9421 gcatctgcct gctttctcaa ctcatcaagt tgcatttcca tctcatcctt tactttaaca
     9481 acatctgttc ctatgatgat ttgaactgat gtattactca atttaattac cccttttgct
     9541 cctgtttttt taatattatc atcattcaaa atagtagcat ccttcacttc taaacgtaat
     9601 ctcgtcatac aattattaag agtcactata ttttcataac caccgcaatt tttaattatc
     9661 tgcttagcca tatatgaaaa attgccatga gaaagtttta aattcttttc tttttcacta
     9721 actttatcac ctatctctat ctcaacttca cgtcctggtg tttgtacatt atcttttatt
     9781 attatatatt taaatgttac ataatataat acaaagaata caattcctat aggtataact
     9841 aaccacgctt tatctccata actaaaactt aagacatagt caattatact tgctccatta
     9901 aatgaaccca agcgagcacc cagtaaatat agtacaactc cacctagacc agaataaaaa
     9961 gaatgaatta tatataactt tggagatgca aataaaaacg aaaattctat aggttcagtt
    10021 actgttgcca taaaagaggt taatgctcca gcagtcattg ctccttttac ttcttgcttc
    10081 tttttatcct gtgctgtttt atatatagca taagaaatag ctggtattga gaacatcata
    10141 attatgtaaa atcctgtcca gaagaatcct gcatttgggt ctcctgccat aaatctagga
    10201 atttcccctc ttataacttc tcctgttgat gttgtatact cacccatttc aaataatatg
    10261 taagcattta aaacttgatg taatccagtt ggtattagtg cacgattaca gaataaaaat
    10321 aagaatattc ctattgcacc agctcctccc aaccaaaaac caaattggtt catcgcagct
    10381 tgtggatata tccataataa actaaataca tatgataaaa ttaaagcaac tactggtgat
    10441 aatgtaatta cacatttttc tcccgcaaag aaacttaaaa atgctggaac tttaacttta
    10501 tatgttctat tgtaagtcca tgctatacaa gctccaacaa ttataccacc aaatacacct
    10561 gcatttaatg tatcatttaa taactctaaa tttgacttaa atatcatata tgcaactacg
    10621 gctgcaacca ttacacttgt cttgtcttta gactttgagt aggcactagc tgctcctaat
    10681 aaaaataagt agtccccatt tgaaaatact atattagata tattagatat agctggcaca
    10741 ttaagcatat ttggaccaaa taataatcct aatatccctg ctgctggcat tacagctatt
    10801 ggcaccagca tagtctttcc taaaagtgac agttgttgca tcattttgtt taagaacctc
    10861 ttcatatcca atcccctttc tatttcggtt taattttttg tgaaaacgat ttgttcacat
    10921 tttatatttt aaatattata tttgtttaaa caaattgtca atatttttta tttattttat
    10981 ttttatcatt tagtttcaca tctaaatcct ttcaagtata ttgctatatc tagtctattt
    11041 ttttaattta tttttttcgg ttttaaaata tgtttaacca tatatttgaa gcaacccaca
    11101 aaataattca attttatcct ttttatggtt tatatgattc tattttcact atacatgtct
    11161 aaacacattt gatataattt gttcatatca gttcaataat attactaaag acctttaagg
    11221 aggggtaggg gtatatggct aaaaataaat tttcagagat atatgaagta ttaaaagaag
    11281 aaatacttga tggtaaatat acaagtaata tgatgttgcc tactgaactt caacttattg
    11341 aaaggttttc atgtagcaga aacactgtac gtagagcaat ttctcaatta aatgcagaag
    11401 gatatgttca aagtataaaa ggcaagggtg ttgtagtgtt agaaaatagt tgttctaatg
    11461 atttttttct aaatatgcac aattttaaag gtgtagaatc gattgtagaa gataaaaagg
    11521 ttaatactgc tactagtgtt ctccatttca gcaaaatatt aattgataat aagttatcta
    11581 aaaagacagg ttttaaggtt ggtagtgaag tatattacct tcatagacta agatatattg
    11641 ataacatccc taagatactc gacataaact attttctatg tagtatagtt aaaaaccttg
    11701 atgtttctat agctcaaggc tctatttata aatatataga agagtctcta ggtacaaaaa
    11761 ttgtttcatc cagaaagata tttaaaattg aaaaagctac ggagcttgag ttaaaaacac
    11821 tacctctaaa tgattataac tgtgttggtg ttattaaaaa tagtgtatat actgatgatg
    11881 gaaagctttt tgaatatact gaatcaaagc atacacctga aacatttgta tttatggatg
    11941 ttactcaaag atattaaaaa attatgttta attaaacata atttttttac gtttagacac
    12001 tttatcccat actaacctca ctattttata tataattata tgtttataca aataattatt
    12061 tattttatat tttagcttca atattagttt aatacttgac aatttgttta aacatattat
    12121 aattaactta taaattacag gaggtatcaa gtatgaaaaa ttggtggaaa aaagcgacgg
    12181 tctatcaaat atatccaaaa agtttcaaag atagcaacaa tgatggtata ggtgatataa
    12241 atggtattat agaaaagtta gattatcttt cttctttagg tgttgattta ttatggttga
    12301 ctcctatgta cgtttcccct caaagagata acggatatga tatagaagat tattataata
    12361 tagacccaaa atatgggaca atgagtgact ttgaaaaact cttaaaagaa gctcataaaa
    12421 gagacataaa aataatgatg gatatggtcc taaaccatac atctactgaa cataaatggt
    12481 ttaaagaatc taaaaaaagc aaagacaatc catatcgtga ttattatttc tggaaagatg
    12541 ctaaacctga tggttctgta cctaataact ggatatccag atttagtgga actgcttgga
    12601 aatatgatga aactactaac caatattatt tacatttatt tgaggaaact caagctgatt
    12661 taaattggga aaatgaaaaa gttcgtgaag aatgttacaa aatactagaa ttttgggctg
    12721 ataaaggtat tgatggtttt cgtttagatg tagtcaacct actatccaaa acacctggat
    12781 tacctgatga ccctataaca ggaccaaaag gagatggaag aacgcactac gcggatggtc
    12841 ctcgtataca cgaatattta cataatatga atcaaaaagt tttcaagcct aaaaatatag
    12901 ttactgtagg tgaaatgtca tctactacac ctgaagagtg tatcaactat actagagaaa
    12961 atcgagaaga actatctatg gtttttagct tccatcatat gaaaacagat tataaaggag
    13021 gcgcaaaatg gactaatgaa atgttctctt tggataaatt aaaaaaagca caatctgact
    13081 ttcaatataa aatgtatgaa ggaaaaggat ggaatgctct attttactct aatcacgacc
    13141 aacctagagc tttatctaga tttggagatg atagattttt tcgtcaagaa agcgctaaga
    13201 tgttagccat aacactattt ggtctacagg gtactacata catatatcaa ggtgaagaaa
    13261 taggtatgac aaatgcctac tttactaaaa tagatgaata tgatgatgct gaaagtaaga
    13321 atgcatacta tcatatgatt aaatctggta ttgatgaaaa ggaggcattg cttatacttc
    13381 aacagaaatc cagagataat gcccgcactc ctatgcaatg ggataatact atttataagg
    13441 ggttttctaa tcataaaccg tggttaaaag ttaataatga caatattagt gtagaaaatg
    13501 agcttaatga ttctcgttca gtattttata cttatcaaag attaatcaaa tatcgtaaac
    13561 aatatgatat ttttacagaa ggtacttata gattgttaga tgaaaatcat aaaaatctat
    13621 ttgtttatga aagagagtat aaaaaacaaa accttcttgt tataagtaat tttactcatg
    13681 ataatgtagt atataaattg cctgaaactt atttgaataa attaaactat acaatacttg
    13741 aaaccaatta cactagagat ataattgaaa atgaaatgga aataaaacct tatgaaagta
    13801 taatgatata ttataaataa agttatttat gtaaaaatcg gtataacatc caggtgttat
    13861 accgatttta ctaaattcaa aataatattt atagaatctc tatagattac aataattatt
    13921 tgacttctac atcattatat ttttttgaat atactttagt catatagaaa tatataggaa
    13981 ctacaattat cattccacct aatccccaaa ctatcttcat aggttcatcg atacctgctt
    14041 ttatcagtaa taatatactt ataataactg caaatattgg tataattgct cctccaggta
    14101 ttctaaaatt tacatcttta ccagcatact tctttctcaa tattagtaca gctaaacatg
    14161 taggaatata ttgaataaat ctcgatacaa cacttatttg agatagtgtt gtaaatgaac
    14221 ccgaccatgc tattagtaga gttcctatga cagatactat aattgctata tatggagctc
    14281 catttttatt agtttttctt atagacctag gcattagccc ttgttcaacc aacgcagcag
    14341 cagaccttgg tgtaaatatg gatgatgcaa tatttatacc tccaacagat actatagttc
    14401 ctattgtaat tatatatcca cctataggac ctaacatctt aatggcagca tctgcaacag
    14461 gagtagctga ccctgctaag ccattaccca acacccctat acttattccc agtataagca
    14521 tatatattac tgatactaca aacattacca ttaccaatgc tttaggtaca tttttcttag
    14581 ggttttccat atcttttgct gcaacagcta aagattcaaa tcctgtaaaa gcataaaata
    14641 aagttataga tactgctaca aaagctgatg acgcactttg acctttttgt attattacaa
    14701 atggggtaaa attagaaccc tttataaaga ataatcctac agctataaat aaaactattg
    14761 gtaataattt tcctatagtc atgatattat ttaagtgctt agattgtttc attcccataa
    14821 aatttataat cccaagtcct acaactaata taccaattat tatattttta gtaagaggtt
    14881 ctgctgcttg tggccataca gatgatagtg ttacagcaaa gaaatttgcc atagtagccc
    14941 aagcaatcat actaactatc cacttcataa atccaacttc aaatccaata aaatctccaa
    15001 atgcctcttt tgcataaaga tatggacctc catcagtatc aaaccaacta cctgcttcag
    15061 caaaacataa agcaatagaa acagctaaaa gcatacaaaa aactaatact aatatacttg
    15121 ctggacctat atctgtataa gcatcaccag gtaacccgaa tatacctgac cctattattg
    15181 cattgattcc taatagtaca atactccaaa aacctaactt aatgcctttt tccattgtta
    15241 ttccccccaa agccaactta atatatttat atttttcatt tagatgcttc tttaactatt
    15301 gccataaata tttttttcat attatcataa tctctagtca tcatttctgg atgccattgt
    15361 atccctatta caaactcact tccttccttt tctatagctt caattaaacc gtctttagat
    15421 gtagctgaaa caatatatcc cggtgctact ttgttgacaa ctaagtgatg aaaactatta
    15481 actaaaactt ctttttctcc taatatttca tataattttg tcccctcctt tatctgtact
    15541 gtatgtgtag gtacgttaga tagatgttgc tgattgtgtt taatataagc accttcaatt
    15601 aaagataaat cttgatacaa actacctcct tcagttacat ttataatttg ttctcctcta
    15661 catataccta aaactggttt tttcatatct aaagcatgtt ttaataattt catatcaaaa
    15721 ctatctctct ttggtgatat tgcccccaac ttattatgag gttcttcttt ccatatgagt
    15781 ggatttacat cttgtccccc agatagtata agcgcatcta tattagacac ttgctccttt
    15841 ataatctcat catcatatac aattggcaca atatatggta tagcttcaca cataactact
    15901 gattgtatat agtcattatt tacataagcc ctttcatagc caggaaacat gcccccttca
    15961 tcaattaata aattacctga tattcctatt actggatatt tctttttaac cactttcttc
    16021 accccttaac ttatttgtga acaataaaaa atgctacttc tcttaaagag caagtagcat
    16081 accaagattt tatttatttg aacagtataa tttattttag aaatactttt tttgaatact
    16141 agtaatataa ataactttat ttacttatat tttattttaa tcctgtttag tttgactttt
    16201 ttaaattgtt ttaagtacaa ctaataggta aaataggcat ttagattttc ctttttatca
    16261 acctattaaa tgctgtttaa tatatttttc ccccttctat ttaattcact tccctttcta
    16321 ccagattcac atattatcaa atccagctct ttaagtatgt taagtattcc ccttacctca
    16381 ccttctgtta tactgtagcc taattcattt attaaattaa caataccatt tctacctata
    16441 tttttattaa gattattggc attttttaaa cactccatta ctaactttac tttctctata
    16501 gatgttctat catttaatat tcttatttct ttactaaaat cttcagttat atttaataag
    16561 ttatgaggta aatcatttat taaaataaca tcctcagaac tcataatgtt aatatacata
    16621 gcagtatttt ttaattctct tatattacct ggccattcat atgtattaat aatatcttga
    16681 acatcttgac taattgctct cttccttttc ataaaatgct ttagcataac ttctatatct
    16741 tcttttcgct ctcttaaagg tggtatattt atgggcaata catttatcct ataatataaa
    16801 tctgctctaa atctatctcc ttttatcatt tctaataagt ttttatttgt agctgcaatt
    16861 actctaacat ctatatctat tacattctct cctccaactc tcattacttg attctcttgt
    16921 aatactctaa gtaatttgct ttgtaagtat ataggcatgt ctcctatttc atccaaaaat
    16981 atagttccat tgttagcctg ttcaaataat ccttttttac ctccttttaa agctcctgta
    17041 aaagcacctt cttcatatcc aaaaagttca ctttcaagta aattttctgg catagctgca
    17101 cagtttatcg ctataaatgg ctgactatgt ctcggtgatg cattatgaat agactgagcc
    17161 atgagttctt ttcctgttcc actttctccc gttataagaa ttgtataatc agattcagct
    17221 attttcatac ctaatttctt agttctttcc atcttcgttg agactgtttt tatatcatca
    17281 aaagtatatc tagcaacttg tcccttttct ctaagcttct ttgataggtt ttgctccaat
    17341 tttcttatgt aagttatttc ttgaatacaa aaataatatc cagtaaccct tccaaaactc
    17401 tctatgttat acttatttac gtttaagtac ttattattaa attttataac ttcatcatgt
    17461 gccttactca aactaaaaat ctttttaata ttctcattct ctaattcatc tatatttttc
    17521 cctataatat tactttgaat atctagtata tcacttaaac tattattgca aataattatg
    17581 tttcccttat ctgataccat tgccatacct aagtttgata aatttattat agaatttaac
    17641 tcatctgctt ttatagtaag ttctttgtat ttagtattta ttcctgaata taaactaatt
    17701 atttcatctg tatatttaat tagcctctca gtaatttctt tatcatccag tttgagtttt
    17761 gctattattt gaataaacgt tgaactatca atatacctat gacctaagtc aataacttct
    17821 tttatatatt ttggaactaa acttgcctcc cctggtgtta tagctacctt aatatttcta
    17881 tattcttcat tgggattgta tggtataaaa ttcaaatgat ttattcctat attgtataaa
    17941 ttagatattg tttccaatac agtatgctta ttatcgttta ctactaatac atctattcct
    18001 tctggcaatg aaaataactt atatatatcc ttttgcttta tactcctatt tatagttatt
    18061 atctttgaga catcttctac atattttttt atttttaata ctctatcatc tatcattacc
    18121 aatattatgt catcttttat caattccttc tctgttattt tatttaaata ataatttttt
    18181 attattactt tatctttaaa tatcatttct agatttttat ttaagaaaca ttctaattta
    18241 gacaacccat cagttactac tgctattgtt ttcattttat tactcctttt aaaatatact
    18301 ctactctttt ataatataca tttgattttc actttaacag cacaaaaaat tgtacattta
    18361 gtatactaac atattccttt attagtttta tgtccctaaa tatcatagtt gcaatatcta
    18421 tcatatttct taaacttcca taaagcaaaa attgtgccaa attaataatt ttcttacttt
    18481 tattctaaag ttcctcaata gtatttacag acttagcact tataacttat aagtctttat
    18541 attattaatc tatttttata ggttaattgt acaaatcata tatcactttt acccattaaa
    18601 ttcattataa aaaagatgga ataacgattc gtcactccac cttttttgta ataaaaaatt
    18661 gtatatataa acactatttg ctactcttct aagcacaaac agtatctaca tactataacc
    18721 tttcaccatt agattctatt acttgtttat accagtcaaa actcttcttc ttaatcctag
    18781 acaagtctcc acttccatca tcatacttat ccacgtatat aaacccatat ctcttggcca
    18841 tctcaccagt agatgcactt actaaatcta tacatcccca tggagtatat ccaaataaat
    18901 caacaccatc ttctacagct tctttcattt gctctatatg tgcttttaaa tactctatac
    18961 gatagtcatc atttatcact ccattagatt cttttttatc ataagctcct aaaccatttt
    19021 caactaccat cataggtatt ctatatctat cataaatttc atttagtgca tatctaagcc
    19081 cttcagggtc tatttgccat ccccattcac tagacttaag atatggattt ttcactccac
    19141 ctaatatgtt gccacttgta ttatcttctt tagaatcact acttacacat gtagacatat
    19201 aataactaaa tgtataaaaa tctacacaac cttcttttaa aatttctaaa tctccttttt
    19261 ccatttttat ttcaatatta ttttctccaa aatatctatt catataagtt ggatattcgc
    19321 cacgcacttg tacatctcca cagaaataat tcattactct actatctctt tgagttttaa
    19381 ctgcatcatc tggattacaa gtataaggat acattgttgc aaatatttgc atacatccaa
    19441 cttgataatt tgaatctgtt tcatgagcta atttaacagc cttagcactt gctacaaatt
    19501 gatgatgtaa cccttgaaaa cgaagttgag gtctatctac ttggtttata aaatctgtag
    19561 tcccttcatt taatattcct tgtgataagt agcctcccat aggcattgta gctgaattta
    19621 tttcattaaa tgtcagccaa tattttactt tgcctttgta tcttttaaat attgtttcac
    19681 agtaattcat atacaagtct attacatctc ttgaaaccca tccattgtat ttctctgtta
    19741 ttgcaaatgg tatttcatag tgagatattg ttattaatgg ttctatatta tattttaaac
    19801 attcatctat tacattttca taaaatttaa gtcctgcttc atttggttct ttctccattc
    19861 cagttggaaa tattctagtc catgctattg aaaatctaaa tactttaaat cccatttctg
    19921 caaataaagc tatatcttct ttatatctgt gataaaaatc tattgcttca tgacttggat
    19981 agaatgttcc tttttctata actctcgtta ttcttttact tactttttga gaacctccag
    20041 tacacatatc tgaacaagaa ggacctttcc catctaaatt ccatgctcct tcacattgat
    20101 ttgctgctgt tgctccaccc cataaaaaat cttttctaag tgccataact ctacctccaa
    20161 taatttattt atagtaaatt tattttaaaa taaaaataca tatatctaat ttttaattaa
    20221 taaaactctt ttttacgcta aaacagttat caattcttga ctattattta cattcttttg
    20281 tgtattttct ataatgtcca catagtcaga gtaatttgta acaactactg gtgtttctag
    20341 gcaataccct tcttttgata tgctttctat atcaaaagtt attaaggtct gacctttttt
    20401 aatcttatcc ccttgattaa caaaagattt gaaatactta ccttctaatt gaacagtatt
    20461 taatccaaca tgtatcaata gttcacaacc attatcagat ataattccta tcgcatgttt
    20521 tgttggaaat aaagtcatta cagtcccatc aaatggtgct accacttttc cttctttggg
    20581 tgttattgct acaccttttc ccaatacacc tgttgaaaat gctgcatctt gcatttgttc
    20641 caaaaccttt acgtaacctc ttattgggct atatattact tctcttttgt ttttttctac
    20701 aacaccttgc ccattgtcat ctttagaaat ttctactata gcatcatctt tccaaagaaa
    20761 taatgttaaa acgaatccta ctacaatggc tactattatt gctattatag ttgcatatac
    20821 acccgtcata tctccagttg ttggatttat ataattaggt ataccaaata ttccaagtcc
    20881 acctattaca taacttttta cattcattat tcccattaca gctcctgcca cagcaccacc
    20941 tacacaacta tatatgaatg gtgtttttct tggtaaagtt attccatata ttgcaggttc
    21001 tgttacacca catatccctg aaataattgc aggtatacac agctctttta ttttcttatc
    21061 ttttaattta aagtacatcg ctgccactac tgctgtttgt gcaaaagatg gtacaaatgc
    21121 tcccactaat attgtgtcaa atcccatagt cataaggttt gacatagcta ttggtactag
    21181 accccaatgt aatccaaata ttacaagcac ctgccacaat aatccagtta atgcgccaca
    21241 taaaattggt gaaaagttaa ataaagctaa aaatccattt ccaactatgt tagaagcata
    21301 ggtagctata ggccctataa ccataaatcc caatggtaaa gaaactaata aaactgccat
    21361 tggtacaaag aaattcttta ctacatccgg tattatttta gcaaacactt tctctaactt
    21421 agctgctaat agtactatta aaattactgg tactactgtt gaagtatagt tcattgctat
    21481 tactggtaat cctaagaaag ttatatatac tggtgattca aatattgttc cagtaaatag
    21541 attgtataaa ggttgacctg tagatgctag agtatctaat tgaatacctg aatagcataa
    21601 aactgcacct ataagcattc caataaatgg ttttaatttg aatttcttag cagctgtata
    21661 tccaagaatt atgggcatat actggaacat tgcatctcca attgcattta acataatata
    21721 tgcaccatca gtttctccat acaagcccaa tgctgaaaat actgcattga atccttttat
    21781 catacctgca gcacacaaaa ctcctagtat tggttggaaa actcctgata ttatgtctat
    21841 aaacttatta agtaatcctt ttggtgcatc tgagtctgaa ttatccactg tatcccctcc
    21901 taatccagcc aaatcgcata caaccgcata aacatctgga acatggttac caataactac
    21961 ttgatattga cctgcacttt tcattacagt aactatacca tccattttct ttagcacatc
    22021 atcatttgct attgattcat cttttaactt aaaacgtagt ctcgttatac agtgagataa
    22081 actgctaata ttatcctttc ctcctacatt tgcaataatt tcttttgata gttgttcata
    22141 ctttctcatt ttttctcctc cattttcttt ttgaaatatg aaggtttggc caacttaatg
    22201 tcaccaccca atatatataa acctaattat taggatttat atctgactta atacttttgc
    22261 ttactactct ttcaacatga atagttaagt ataacttttc ctcatctgtt aaatcgtaat
    22321 tgtatttttt atgtataaat tcatctaact tttctatgca tttataagca tttttatatt
    22381 tgacttttat tacatttaat aaatcatcct cattactttc atgtaaatta ttactaacaa
    22441 ttctttctgc aaaatacttt aaatgagtga taaatctatg ataataaact gaatcttcat
    22501 caaatactat tctataatgg tacttaacca catttgcgat ttcttgtatt atttgtgtaa
    22561 tttcatatat aatttttata ctttgatcca tatgtacatt aactatatgt aatgctataa
    22621 atccagcttc atcttctgac agtctaattt caaatttttt ttctatataa tctaaagcct
    22681 ctaaacctat tttaaactca tttttataaa atctttttat atcccaaagt agtacatttt
    22741 ttacatttac tccttcacga gctctttcta ttgcggtata catatggtct gttaaagaaa
    22801 tgtaaatact atcatttaat cttttcccta atttttcttt agcatataaa attatttcat
    22861 ctgacaattc catatatctc ataggaatat ctgctattaa ttctttaaat ttatcagata
    22921 tatttgggtc tgaaagtttg aatatcttat caattttatc ctttgatata tggtctcctg
    22981 tacgtttttt ataagctaaa cctcttccca taactactat ttccttatga ttttcatcag
    23041 ttgtaataat tacattatta ttgagtattt tttcaataat cattgtgatg ccccctttat
    23101 tagcattaag ttaaaataaa tcttttttaa ttatttagaa tatttaaaat gtaatttatt
    23161 acataaaaaa aaacctattt attacacatt aaatagactc ccatgagtcc tgagtaataa
    23221 ataggtttag cttgtattta aacaatcact atccgtttgt attttatttt aattattatt
    23281 tattatatgt tagcaaattt attttttagt gtcaactttt tctcaatatt ttaattttaa
    23341 ataaattata taaatttaat ctacaaagta ctatttttat caataaacaa aataaaaaag
    23401 gtattacttt ctttaagtaa tacccattta tttttaattt aagtggttct tcttaatctc
    23461 atcaagtagt ccatcttcta aataaatttc taatgcacat cctaccattg cctttactgc
    23521 tttgtgaagt ttatcataag cttcaccaga atttgcatag tttagaaatt ctttttcatg
    23581 aacatatacc attggactta tatctaaggc tatttctgta tacatagtag ggcaaacttg
    23641 acttacatta cctatatctg tagaacccac tggaccatct ttaccttcta atatatctgt
    23701 tatacctacc tcaatcaaat tattctttgt tatcttttga agtactttgt tatttattat
    23761 gttgtcaaaa gaattttcaa agtatctata agaaagctta gctcctgtca ttaactctgc
    23821 accttttgca cagtttataa cttttttagt tacttcatct aaataacttc tttcagcagc
    23881 tcttacgaaa aacttacaag ctgctttttc tggaactatg tttggagcct ctcctccatt
    23941 tgaaactata ccatgtattc taacatctga ttttacatgt tgtcttagtg catttatccc
    24001 agcaaaagta agttgtactg catctagtgc attgactccc atatgaggat gactagctgc
    24061 atgtgctgat acaccttcaa aactaaactc taatgaatct atcgccaaag ccttacagtt
    24121 tatattatta aaagcttcta tatgcatctg ataacttaca tcaacatcat caaaagctcc
    24181 tgccttaact aaatcacatt ttcctcctgt tgtttcttca gctggtgttc ctattagtac
    24241 aatttttcct ttaaaatttt cttttaactt acttaatact atacaagcgc ctagtgtaga
    24301 tgctgcaatc cagttatgtc cacaagcatg agcaggttct ttattttctc catatccagg
    24361 aagtgcatca tactcagcta aaaatgctat agtaggtcca tcattatctc ctaattcagc
    24421 tctaaaagca gtctccatat tacaatatgg ataagttgta tcaaatccat attccttcat
    24481 tttctctact aagtattttg atgatatgta ttcttcttcc cctaattctg gattattaaa
    24541 tataaaatta caaacttctt caatttcttt tccaagttca ttctctatct cataagctct
    24601 cttctttata ctctctatca taatttacct cctactagaa tcctagatat aatcctattg
    24661 tcaaaaatac cattacaaga attgttaata ctaccattaa aggaactacg aatctaaccc
    24721 actttgacca aggaatattg gctactgcta gtgctcccat taaaacacct gatgtaggtg
    24781 ttattaagtt agttacacct ataccaaatt gataagctgt aactatcatt tgtctggata
    24841 catctactaa gtctccaagt ggtgcaagta cagggattgt aagtgatgca agccctgatg
    24901 ctgatggtat taaaaatgct attaatcctt ctattaagaa agttaaattt ataaatatag
    24961 tttttggtaa tccatttaat aaatttgctg ccgaatttaa aattgtatct attataaatc
    25021 cattttctgc tattattacg attccacgtg caaatgctat acacaatcct gctgtaatta
    25081 aatctcctgc tccacttata aatgaattaa ctatctcatc ttgtttaagc cctgatgcta
    25141 ttcctgatat tatacctata gctgtaaata tcattgcaat ttcagttatg taccattcta
    25201 gtcttaaaac tccatatatc ataatcgcca tacctattgt gaaaattgct attaccatgg
    25261 cttctctttt tgtaaattct tttatgtttg aatcactatt aaccattgat tcttgattta
    25321 gtgatatatc atgtactaaa gattttttag ggtctttttt aactttattg gcatacatca
    25381 taacaaatcc tatagatata gccatatata ttataaacat aacaactcta aattctattc
    25441 cagaacctgg tactatttgt gataatgctt gtgctattcc cacagagaaa ggattagtag
    25501 tagctgctcc aaatccagct gttgctccta taaatactgt ggccactgct gtcaatgaat
    25561 catatcccat actggtcata agtggaataa atatcatata aaaaggtagt gtctcttcac
    25621 tcattccaaa tgtcgttcca cctagtccaa atagaatcat tgaaatcggt attattaaaa
    25681 attgaaactt cttcatttta tttacagtat gagctataac agcttctata gctcctgttt
    25741 tattaactat cccaaaagct cctccaacta ttaaaacaaa cccaactact tcagccgcat
    25801 ctatgaatcc gtttataggt gctgtaaata catcagtaat tccttgtgga tttgatggtg
    25861 ctttttcata agttccagct acaacaactg accttccgtc tatatctact ctttcaaaat
    25921 ttccacttgg cactatccaa gtaagaattg ccattaacac tattagcaaa aatattattg
    25981 taagtgtctg tggcatagta aactttttct tttttaaact gctcattttt aatctcccca
    26041 ctttttaaat taaaatattt tattattgca tccctttatc ctattttgta tagatgtaaa
    26101 aaagctcaac atggatacat gttgagctaa atgtgctaaa tacaacatac ctgcatcttc
    26161 cctttatctt gtttaagata gctctccatc aaaattcggg ataattttga tgacagtact
    26221 gtacctatta aatacagtcc cagcactagc ttcggacact agcacttcgg cacttactcc
    26281 ttttatattg cctcattggt ttccaacctc ttcaatatta atcttagtaa atgcaacctc
    26341 tacctcaatc tgttcgttag atgaggtaat tcttattaat ttttattaat tcaaagtaat
    26401 tgtagcataa tatgtcgaga ttgtaaatat tttcagtata ataattctta ctttatgata
    26461 tcattcactt tttcacaaat aattttttct tcatataatt taaatactgt agtatatttt
    26521 caatacacac aaacttcaca caaatttaaa actcttataa tacctttata ataaaagata
    26581 aaagccatgt aaacttatca atttacatag ctttatatac attaatctac tccaagtaca
    26641 gctttaagct ttgatgctat tgtaataact cttggaccaa atataacttg aactgagttt
    26701 ttctttttaa caactccact tgcacccaat tcatttaccc agaaaccatc ttcatagaat
    26761 ccatcaattg aatttagctc tactctaagt ctagttgcgc agtttgtaac attttttata
    26821 ttattacttc ctcctaaacc ttctattata gaatgtacaa gcttcgtatc tttatcatct
    26881 acttcctctt ctactgcatt gttgtatgct gttttaccag catctacaga ttgtctttct
    26941 ctatattctt tcttactata aagcttaact tcttctgaac ttcttcctgg tgtttttata
    27001 tcaaacttct tgattgccca tgtaaatatt atataagtaa ctataaaaaa tattggtatc
    27061 aaaaataata atggtaacgc atccactttt tgtggttgta ataaattagg aaacatattt
    27121 ctaaatgatt ctcccattat agaaacatta aacatttctg taaatacata cgtcaatcct
    27181 gccagtggta catatgctac aaaatacaat aatggtgcaa caaataagaa tgtatactct
    27241 attggttccg ttacccctac caacatggct gttactattg ctggtatcaa tatgcttatt
    27301 gcttttttct tattagcatt atcagccaac ttatacatag ctaaagctgc tgctggtaat
    27361 attgcaaaat gtattaataa acgacctgtt gtgaagttac gagttatata agatagtgta
    27421 tctgggtctc ccatttgagc taatcttata ttattaacac cctcgtaaat tgctccacct
    27481 acttccattg ttccacctac tcttgtatat tctataggga atgcaattaa gtgatgtata
    27541 ccaaatggta ataatgcttt atccattgct ccaaaagtaa atgttcctaa taatccacta
    27601 gaacctatcc aattaccaag tttagataat gctaacccta ctggtggcca tataaagtac
    27661 attgctgtac ccaatactat tgatgctata aacatcataa tcattgaaaa tcttggacct
    27721 gagaaaaaag ataatgcact aggaagttct atgtcaacaa atttgttatg tactgcacta
    27781 gctacaaatc cagctattat agctccaaat atactacaat catatgaaaa tattcctaag
    27841 aaagaaccat ataaagaact tcgactatag gcttctgcct ctcctaatcc agatgccata
    27901 aacgcttctg ctgtaactgt atctgaattt ataccatttg ctgctaataa tgtacttata
    27961 actgtgtgca ttcctataaa taaaacaatt cctcctaatg caccccatcc tttttccttt
    28021 ttagataatc caaaggctat acctgtaaca aagaatattg gtaagtatct cattaccata
    28081 aatcctaagt cgtttattac tttaaagaat acatttataa atgacccttc ttttattagt
    28141 ccactaagcc ctaatgcaga aacgttttcc atatttgcaa aagctgaccc taatcctata
    28201 aatactcctg ctacaacaag taatataaca ggtatcatca ttgctccaga aagtacttgt
    28261 aatttatcct tcatagtatt atttcctcca tttttattga aatgccaggc tatataatgc
    28321 atatatatat acttctattg ctttgtataa tgtatctata cttatatttt catttgcctg
    28381 atgtgaattt ggattatccc cctctattgt gggtccaaat gcaactccat tttttaacac
    28441 tcttgcataa gatgcagctc cttttgtaaa aagttctgct tcttttccaa aaccttgttt
    28501 atatgcactt gataatttat ttattaattc actatctggt tttacataca gtaattttaa
    28561 atccttatat atatcaacta ttaaattttc tttttttcca atttcattaa ttctattagt
    28621 tataaactcc caacttatac ccttaggata tctaaagtct attttcatat tcaattcatt
    28681 tttctctaag aagatactca ttatacatat tgtagaaact cccatatcta aatcttcttt
    28741 atatatccct aatttttttc catgattatc atctgtaaag tatgaattta aaatatccct
    28801 tattataacg cctttatcgt ttaacttttt aattttctct aaatcttttg ctaaactaaa
    28861 tgcacagtta tatgacctgt gtggatttct cgataaagcc ctttctcctg tatatcttac
    28921 taatacttta ccttcttcta gttcttctat ctctgtataa taagctaatg attctactaa
    28981 atctttctta tcattagttt taaaaacagc ctcaattttg tcacatacaa atccatcttc
    29041 tttgtttgac tttatatcaa ctaaattgtg atttctaaat ttatctttat ctattttctt
    29101 tcttagattg aagtataaaa tacctttttc tccatttact attgggaagt ttccatctgg
    29161 agaaaatgca aattttggct gttcattgac actaaagtaa tgctccatac attcccaagt
    29221 cgtctcttca gccccaccta ctatcaatct aatctttctc tttggttttt cattaagttc
    29281 tcttaagaaa agtaatgcat ataaaatccc tatcaaaggt cctttgttat cattaactcc
    29341 tctaccataa agatagttat ctttttgaca tactttaaaa ggtttactct tccaaagttc
    29401 cttattatat attggcacca catctatatg attaagcacc cctactactt cttctccttc
    29461 tccatattct atatgaattg catatccatc aaagtctttt acaacaaaat ctttgtcttt
    29521 tgctatttga ataagcttat cgaaggcatt tcttatttct atcccaaatg gttgatttat
    29581 atctgttgta ctttcatctc taattgaagg tatttcaact aaacttctaa tatcctttat
    29641 gatattttct tgattggatt ttaaataatc aataattagt tctttattta ctatcatctt
    29701 aattatttat caccccttta tattaatttt acaaagcatt tcagttcata tctacattat
    29761 aaattttgaa tattattttt tcaatatttc aaatacttct ctaagttacc ataatgatac
    29821 ggtaaacaat caacagatta tgacaaaatc atactatgtc ttttactaat attttaactt
    29881 atctattacg tcttttttaa tgtaatttta ttccaaaaaa tacattaaaa aagatgtagc
    29941 cttagctaca tcttcttata tctattcaac ttccattatt atgctattaa tatctacatt
    30001 tccaggttct atcctatctt ttactgttct cttaacagaa tcagcatcat ccattataac
    30061 tacaggagtt attatattta aatcatttga cattataaag tctaaatcta cttccattat
    30121 taaatcttta gcttttacct tatctcctgc ctttacctta acatcaaacg gctttccttc
    30181 tagtgacatt gtctctaaac ctacatgtat caatatattt gtattatcat tgctcttaat
    30241 cattattgca tgctttgtat caaatacatt gactatttca ccatcaacag gagaatataa
    30301 cttaccttca cttggtatta ttgctactcc atctcccatc atcttttggg caaaaactgc
    30361 atctggaaca tcttctaagt tcactaaatt tcccttaaaa gggctatata ttttctcaat
    30421 cttattttct tttttcttaa atatattaaa cataacacac tctccacatt ataaaatttt
    30481 atgataatat ttttcttaaa gcttttgcta caccatcatt tacattagta tcagttacaa
    30541 aatctgccat agattttact tcatctgtag catttcccat agcaatacca agacctgcaa
    30601 atttaatcat ggatatatca ttttcattat cacctataca aataacttct ttgctatcta
    30661 tacctaatat atcacaaaat tttttaactg caataccttt tgaagtacct ttactcataa
    30721 cttcaaaatt tatctttcca gaacttacta cttctaaatc ttcttgtttt ttaaactctt
    30781 cctttgcttc tcttaaagaa tctacgtttt ctgagaaaag tatacatttt aaaatttcat
    30841 tatcagcttt ttctaataaa gtttcacaac ttgcattttc tattatttca attctgtctt
    30901 cttctttatt cttactattt attaatctat atggatgttc ttgacctatt tcattgttgg
    30961 atataactat tttacagcct ttaaaatgtt tatttaaatt atatttttca cctattgtat
    31021 agattttctt tagagcttcc ttgctcaaaa ccttcttata ctcgtatccc agtaacttaa
    31081 aatatgtccc attagttgat ataatatata tatcatcccc taaaacactt gtaaaatatt
    31141 tcatagcatt ataaggtctt cccgtaacta aggctacctt gatacccttt tccatggctt
    31201 cttttagtgc tttaacattt tcttctgata cttcaaatcc tttacccatc aaagtaccat
    31261 ccatatcagt acaaattaat ttatatctca tttgtatcac atcctagtaa gatttttcta
    31321 atatttcaac aaggtcttcc ttctttccaa aaggtaaaaa tcctaaaact ggacagcttg
    31381 ttatttgttc taattgttcc tttgttacat taaactcaga catttttgtc ttaagtccca
    31441 ctctctttaa aaaactttct atttcatttc ctaaagtttt agctgcttca tcatcttcca
    31501 ctttttctaa ttcttggttt agcattctcg ctactttggc aaatttcttt atattatcct
    31561 tatagaactt ttctgcaaat tttgggaaca ctatagccaa agcctcacca tgaggaatat
    31621 gtgtaactcc tcctattatt tcacttagtg gatgtggtgc tgctgctcca gaatttgcca
    31681 gtgaacttcc ccctaatgta tctgctactg ctaactttgt tctgtattct atattatttt
    31741 tatcttccat tactttaggt agatagttaa tgactaattc catagatttt tcagacatta
    31801 tctctgtaaa gtcattagca tttggattta tataactttc aaatgcatga gtaaaagcat
    31861 caaatgctgt tgatgcagta attctatctg gcaaagttaa cataagctct gggtcaacta
    31921 tacattcttt tgagaaacaa ttttgatgaa atattgtatt tttctcgctt cctctcgata
    31981 ttactgctgc ttgtgtaact tggcttcctg ttccagaagt agttgataca gctattaatg
    32041 gtaattcttt ttcacttacc ttatcgtata ttttaaatgg atttgtatat gtactaaaca
    32101 tataatccca atctattttt tctaaaccaa aagtcaatgc taatactttt gcagtatcta
    32161 tacttgaacc tccaccaaca gctaaaacaa catctacaga ttctttttta gctaattcaa
    32221 acccttcatt tattaattct acagttgggt ttggctctac tctatcaaag tgtacaactt
    32281 ctataccttt ttctattaaa ttcaatgtaa ctcttttata taacttgtct aatggctcta
    32341 catttggagt agtaactaac aaacatttct ttccatattt tcctattatt ctaggtaatt
    32401 cacttaaagt tccttgtcca aaatttattc ttgatggttg ataaaagtta atcatatttt
    32461 tcctccctta aattattgcc ttcatattca aattatacat atcataggat ttttaaggca
    32521 ataccttcac tgttaactct tatttactaa agcttatggc aattattaga caattaatgt
    32581 tttattatta ccatatcatt atgtaaattt tattaagtac ccacgttata attttataat
    32641 caatcttatt gtataattga cttaataata aaaatagagt atatacactt ttttatgaca
    32701 aatagcttaa aggagaatta aattatgaaa tatttgatag acagtgctaa tcttgatgaa
    32761 attcaaaagt gtttaaagta tggatttaag ggaataacag ctaatccatc tatgtatctt
    32821 aaagagaagg taaacttcta tgaatttatt gagacatgta ctaatttaaa tccagaaata
    32881 ttaactgctg aagtcatcgg aaataattta tacgaattaa ttaattcatg tgataagata
    32941 ttaaatataa ataaaaacac tataataaaa ataaatttct ctgaagttgg cttagaactt
    33001 ataaacatat tgaatagtaa aggtgtaaaa actgcatgta ccttgatttt caatgtaaat
    33061 caagcatcat tagctataaa tgcaggtgct gattacctat tcccttttat aggtagaaat
    33121 gatgaaaatg ggaatgatgg tataaaaaac cttacagaaa tatgtaacct tatatcaata
    33181 aatggatata atactaaagt tgttgcagct tctattaaaa atgtattcca cttaactcaa
    33241 gcatctcttt gtggatgtga ttatgcagct gtaacatatg atttatttaa aaaagcttct
    33301 tttaaccagc ttacagtaga tggtgccaat acttttgaaa gagattggtc caaggttaat
    33361 aaatagtaac taagatttag gagggtttgc attgaacaaa agattattag atattgctga
    33421 attattactc aacagctcag actatattac tgtagatact attgccgaag aattaaaagt
    33481 tagtaataaa actattcgta atgaccttgt tattttagat gaatggcttt tagagtttaa
    33541 tttatcccta gataaaaaaa ctggttcagg ggttattatt ctaggtaatg aagatgtaaa
    33601 acttaaagtt attagagata tcaatgataa atcaaactgt atatatgcat actctcctga
    33661 agatagaaaa aggtatattc taagtaaatt atttattaaa aattctaagt ttaggattcg
    33721 tgatatttgc aatgaactac atgttagcag agctactgtg cataaagact tggtagttat
    33781 tcagaatttt ttacaaaact ttaaagtgac tttagttaga aaaaccaata atggagtata
    33841 tttagaggga aaagagaaag atattcgaaa agcaattttt gaacttgcta cggttaataa
    33901 aagttatact gaacttaaag aaatattatt ctcgaaatct aatgaatgca aagatacccc
    33961 atcaacaaaa atatttaagg agctatttaa ctatgatttt gagaaattat ctcaaatcac
    34021 tctacacacc ttacaagtag aaaaaagtaa ccttagtgat gagtattata taaacttctt
    34081 aatacatata gctatatgta ttaaaagaat ttcaattaaa agatatatta acttatctga
    34141 aggttttttt agagaattat tagaacatga atactttgaa aaatcaaaaa ttttatgtgc
    34201 taacctaagt gaagcttttg acgtagaatt tattgatgaa gaggtctgtt atatccttct
    34261 tcatatagaa ggattactta aatcaataaa gaaaactact actcttgaac ctcttaaaaa
    34321 tgaagaaatt acagagtata atctatctga agcaatagct gttcattggg gaagtatact
    34381 aaatttaaac ttaaaggatg atttaatatt aataaagtct cttgctaatc accttaaatc
    34441 agttctacac agaataaact atggatttac tattacaaat cccatattaa atgatataaa
    34501 gagaacattt ccttatactt ataaggctgc taaagagtct aatactgtta tcaaacagtt
    34561 gatgaattat gaagtcaatg atgatgaaat aggatatctt gctttatatt tgatatcagc
    34621 aatagataga agtaaatctc ccctaaatac tattttaata tgtcactgta gtgatggtgt
    34681 atctaactta ttagtccaaa agttaagttt tgaatttaat cagattaata tagttaaatc
    34741 tatacctcta tcttctattt catttacgaa ctttgatgat gtcgacttaa tactaaccac
    34801 tgctcctgta gactttgaac ataatgctga actaataaat ataaatgctc ttttaagtaa
    34861 gaatgatatt tctagactta gtacaattat aaaaaaacta tattctgaaa aaaataaaat
    34921 attatcttat aaataagcaa aaataggctt gtctcaacat agagataagt ctatttttca
    34981 ttgtccaaat gataaaaata tatttgatta catagaaaaa agtgcctcat gaactaaaaa
    35041 attcattttg agacaccttc attttttata tttaatcttt ttatttaaat tttcatattt
    35101 aaagtaagtt ttattatttt attgagcgca agcactattt acattattaa tttcttcagt
    35161 attcttacca aactttgtag ttacaaatcc tattgttttt attatgagta ataaagatat
    35221 caatgaatat atagatatca taaagttctt acttattatt ccagacgcta ctattataaa
    35281 tccatctagc ataaataata taactggcaa ctttagaaac ttaatcacct tattaataag
    35341 cattgcaact aaatcagttc caccagtact acagcccctt attattgcta tccctatagc
    35401 agttcctacc aataacccac ctatcactcc tgctactaac agattgtcag ttatgtgagt
    35461 ttgtggtatt acttttaaga ataatggacc cataagagtt atatataatg tttttattcc
    35521 aaaacctttc cctaaaaata ataatcccat tactaaaagg ggcatagata ttcctaacga
    35581 tatattctca acaggcattg ataagaatgt actaagtacg actgataatc ctgttatacc
    35641 tcctggagct aatgtatgtt ctgcaaagaa aaaattaact gctaatgcca ttaaaaatgc
    35701 tgatattgtt atcagtaaat aatcttttat catttctttt ttcatttaat aacctcctat
    35761 gattatattg tttcagtata aaaaatcaat taaattagtc tagcttcctg tagtttttca
    35821 taaaattaat gcttccttaa aacattaaga aagcatctct tttatcatag tttattatct
    35881 tattctaaac caaactttgt agctgcttca tctatagctt ttctacaatt cttaacacct
    35941 gctgttatac catctttatc cttgaatatt gctgaagata atattacaac gtctggatta
    36001 cattctatta atggctccat attgtctgct cttagtccac catctattgc taattcacac
    36061 ttaggattct tttcgtctat catttttcta gctcttttta ctaaatcaat agcactcttt
    36121 ctccatcccc agttatcttt tccatcagtt tcatcaacac catgaacgac tatttgcaat
    36181 ctatctatat catatatagc ttcatcaaca aaacatagtg gtgtgtaaca tccaattgtt
    36241 aatcctactt tcataccaaa ttctctacag taatttatta tgtatgcaag aggagcacct
    36301 aaaaaatgtt ctgctggtat tatcaacaag ttacatcctg ctgctgctat tttttctata
    36361 aataatctat cacaatctct tgtgtatata tggcattcta ttggtttatc tgtcactggt
    36421 cttattgctt ctattatttg atgaccaccc ataagttgca tattttttaa gtcatgcata
    36481 tctgctgcat ctgaatgtac gtagtctaca cctgcatcca ttgcttcttt aactacggct
    36541 gatatatgac cgtaatttac atgtgctaat cctgctgcta tttttatgtg tttcatattt
    36601 gtatacctcc atattttact ttaattagta ttaaatatta gtttttgata tttttttaat
    36661 attttttaat ctagtattac ttttatttag cattactttt attgctgtat atatttcttt
    36721 tatgaactta ttctgctttt gttaactgtg aacttatttt gctttgtaaa ttaatattac
    36781 actgatatat tttttttcac aacactttga ctactcctta aaagtacctt tatattgtgg
    36841 taaattatag acattaattg tatataaaaa gttagttttt taccacattc ttatggcatt
    36901 tattgtctat actaactcaa ttgatatagc tagttcattt aatatataat cagactataa
    36961 atataaattt actcgataaa aagtaccatt cataacaata ttaatgagag ggagatgtaa
    37021 aatgagaaac tgtattattg cccaatctgg aggccctaca gctgttatca atgcttctgc
    37081 agttggtatt tatgaaaaaa atttagaaac taaatacttt gataaagtat attttggtat
    37141 caatgggatt gaaggtatat taaacaaaaa tataatagat tcagactcct taaacaaaga
    37201 taatgtatta aatttaaagc aaacaccttc gtctggtctt ggttcttgta gatacaaatt
    37261 ggctgattat aaaataaatt cttatgatta tgatagagtt ataaatatac tagatgaata
    37321 tgaaatagat acattatttt acataggcgg aaatgattct atggacacag taatgaaatt
    37381 ttctcaatac tgcaaaaaca ctacttgtaa aataaaattt atagggattc ctaaaacaat
    37441 agataatgat ttattaggaa ctgacaactg tcctggattt ggttctgctg caaagcttat
    37501 ttctacacta ataatggaaa catacttaga tttttctata tacaaaaata atgggatatt
    37561 tatagtagaa actatgggta gagatactgg atggttagca gcttcttctt cactagctag
    37621 acttaatgat actccacttg ttgatttaat ataccttcca gaaattgatt tctatgaaga
    37681 taagtttata gaagatgtta gaaaaatatt taaagaaaaa aataaggctt atatagttgc
    37741 atcagaaggt ataagggata aatatggcaa ctttatatgt gaacaaaaaa cttctgctca
    37801 tgataaattt ggtcattctc aattaggtgg agtaagcaac tatctaaaga atcttatagt
    37861 atcatcatca attacaacaa gagtaaaaac cttagaactt ggtgtaatac aaagatgtgc
    37921 tatgcattta gcttcagata ctgatttaaa acaagcagct gaagttggtg catatggtat
    37981 agaactatct aaaaaaggat atacaggagt tatggctatc attaatagaa catgcaataa
    38041 tccttataag tatgaagtta gtcatactga tatttctaaa atagctaaca agacaaaata
    38101 ttttcctaaa gagtggataa atgaagagtg taattatata actgatgaag ctctaaatta
    38161 tctatatcca cttattcaag gtaaaccaga tatcaaatat aaaaatgggc taccttattt
    38221 ttcaattatt tactaggaaa ttattataat ttgatataca ctcaattcta tcgctcttaa
    38281 atacctaagt ggctgttaaa taaaattata aattgttata tgacagtcac ttaatcgctt
    38341 aattatttaa ctgtttagtt ttttaatttt ttaatcattt ttaatactaa tacgatttgt
    38401 tgtaataata tctcacaccc attttcattt tggatagatt ataattagtt agacaataaa
    38461 tattttgtca tgatattatt atatatagtg atacagatag aaatactttt atcatggcta
    38521 gaaaaacaaa gattacacaa aggatttaaa aatcaaacaa ctttaattaa aataaatggt
    38581 taaaataact tctaaatcaa ctacataaac aataaaagaa cataaagtag ttaaatctac
    38641 aattataaaa ttagtaagac tataacaact taaataaaat taaagttaac aaaagactat
    38701 taaagttagc aaatattcct ctacctaaat aattgctttt tataaagagt tatttatttt
    38761 aattgaattt aattgaaata cgattttaac tagaacaata gattaatgta atatttaata
    38821 taaaataatt ataataattg aaaaggaagt aatctatgat gaaaatagca gtcataacaa
    38881 ccgaattttt aaaagagttt gtagataatt ctataaaaaa gttaaatatt aatgcagaaa
    38941 ttgaaatata tatctataga gatttttctc atgttggaga tttatattta gaaatagaag
    39001 ataggtttga tggatttgca gttagtggcc ctatccccaa aaaagctatt acaaaaaagg
    39061 ctggaactat taaaaaacct ctagttgatt ttggtactga tttgcaaagt tactatgata
    39121 tgtttctaaa gcttattttt aaatataata cattagattt ttctagagca tactttgact
    39181 taacagactg ggcagataat ccaaaagata tatcatatta tttatcaaat ggcacatttg
    39241 gagacttaat ggatagtatt gatgaaaatg catctaaaat gagtttatct gacatagcaa
    39301 aacttgaaaa aaagattgca aaaaaacata ttactctatg gaatgaaggg aaaattgatt
    39361 tttctactac tcgttttagt agtattgttc ctatgttaca aaatgcagga gtaaactttc
    39421 atttcatata ccctgatata gatttattaa aaaatacttt taataactta ataaaagata
    39481 ttcatttaca tagaatgaag ggaaatcaac caagtgtaat atatataaaa gttaaaggta
    39541 caaattatga tgattctatt ttagagcaag tttataaaat attaaatagt attaaaaaag
    39601 ataaattaac cgattttata attgaaaaaa atagtaattc ttttaaaatt ttcactaaat
    39661 gtgaaacgat taaatcatta actaagaatt ttactacatg ttatataaaa gaatctattg
    39721 aaaatagtac aaatattaaa gtatgtgttg gatatggaat tggtaatgat ataaagcaag
    39781 ctatgtctag tgctataagt gctaataagg aatctaaaat aaatccttta aactacagtt
    39841 tcttagttaa cgaaaattat gagacaatag gacctttact atgtgataag ttacttactg
    39901 tttcaaataa agtaactcca catataaata caattgccaa aagttctaaa ctgtctactc
    39961 ttactattca aaagattata tctgttactg aaattctaca aagagatgaa gttactccta
    40021 aggaattatc ttcatgttta aatgtaaccg ttagaactgc taatagattt ctaagtagtc
    40081 tcttaaaatc tggaaacgct actatattat atgaaaaaca aaactcaaca aaaggtagac
    40141 ctgaaagggt atacaaatta tttatatcag aaaataaata ttaaaatcca aaatacataa
    40201 aaatatttta tcatctaata ttaagtaaaa ttatatatga ttttctaaaa ggtatatctt
    40261 tgcatctttt taaggtatac cttttatgat atttaataat attttatgtt attatctgct
    40321 aaaattattt tctaaccatt ttactgcaca atgagcaaat aaagctgaac cataaactaa
    40381 tccctcttca ttaaaaattg ccttcggatt atgcatacca aaattatatc cctgattaac
    40441 acttcccatt ccaatccata aaaatactcc tggaaccctt agtaaaatct ctgaaaaatc
    40501 ttccgaaccc attgcagatg gaaagtctat tacattctca tttcctataa cttctttaac
    40561 ataagataca atctcatctc ctacatccaa atcatttata actgcaggag tccccatttt
    40621 aaactccact ttagcaattc ctccaaatga tttagctatt cctgtagata tttcaataat
    40681 tctatttttt aatttttctc taacaaattg gtcaaatgtt cttaaagttc ctgacataga
    40741 tgctttattt ggtatagcat ttggtacgct tcctgcatta aacataccaa tagttaaaac
    40801 tacaggctct ttctgaggtt tttctcttga aagtatagtg tttaatgcta tttgtatatg
    40861 tgacattatg tttattgggt ccactccaaa ttcagggctt gctccatgag ttgcttttcc
    40921 ttctactgtt atttcaaata tatctgcaga tgccatagct ggaccacgtg aataagctac
    40981 ttgacctgtg ttaagaaatt tgcccaccat actgtgaaat gccatagctg cgtcgacctg
    41041 aggattttct aatacaccat catcaatcat tgattttgca cctctggtgg tctcttcatc
    41101 tggttcaaat acaaacttta ctctaccttt aagtaagtgc ttattatcca taagtatttt
    41161 agctgctcct aagagcattg ctgtgtgatt atcgtgtcca cagctatgag atgcaccttt
    41221 attcttactt ttaaaaggta aatccgtctc ctccataata ggaagcgcat ccatatctgc
    41281 ccttaatagt aaggtctttc caccattttc actgtctaaa acagctacta ttccattatt
    41341 tgatgttaat tctggagaat atcccatttc ttctaacatg ctggtaacaa atgtaactgt
    41401 tctcggtaga ttgcaaccag tttcaggatt ttcatgtaaa tatcttctcc atctaataat
    41461 ttcctcttct attgactgtg cttctttaaa aaattttttc atattccttc ctcctaatca
    41521 gatagccagc aaaatctcac tttcttttag taatgaaatg atgctggcat aaaaattata
    41581 attttatata ttaaacttca tgtcttttta aggagttgga atatcatctg gaatagggca
    41641 tttatatgtt ttaccattca tagatttatc aaattcttct tttgcccttt tagctatctc
    41701 tggattattt aatatcttag ctccaaacat agccataatt tttgatgcat atatcatacc
    41761 tttttctcct atagagcttc ctgcacaaga tgtaaactgc cagctatgac ctggtgctcc
    41821 tagattcgta caccctgtaa aaaagtatgc tgtcggaact atatgcatta catctccaat
    41881 atcagtagag ccataactat taaagtttgt aacatttcct ccaccataat atagatgagt
    41941 tccttctggc aaattatatt tacttatcat agccttatat tgttggggac tttgtctatc
    42001 taattcagct gcaaaatcca attcgtcttg agtccaaggc tcttgtttaa tctcattcat
    42061 acattctgat acaagatttg ctaaaacatg attatttaaa gtattataac aacctcctaa
    42121 aaactctact tcaacttcag tttcagtcat cattgcagct ccttttgcta cctttacaag
    42181 tctttcatat gtactttcta cagcttctct acttaaagct cttgtataat accatactga
    42241 tgctttatca ggaactatgt tgggtgctac tcctccatct gtaattgtat aatgaatcct
    42301 tacatcagaa gtaacatgtt ctctcaaata attagctccc acattagtaa gttctacagc
    42361 atctagtgca cttcttccat tttgagggtc tgaacctgca tgtgcagtct ttcccttaaa
    42421 atgaaatttt acagaattaa cagctgttga agtaccaatt gtagcttcac taattttatt
    42481 tggatgaaaa tttatagcta agtctaaccc ttcaaatact ccacctctag ccataaatcc
    42541 tttacctgtt aattgttctt ctcctggaca tgcataaaat actatagtcc cacttaagtt
    42601 attttctatc atctctcttt taagccctac tactgcccct acatgtgctg tacataataa
    42661 attatgccca catccatggc catatgcacc caattcaaat gcttcttgct ttgttgaaac
    42721 tttttgattt aaaccaggta atgcatcaaa ttctcccata aaacctataa ctggtttgcc
    42781 tgaaccaaaa ctagctctaa tagccgtggc taaacctcct acacctactt ccacatcaaa
    42841 tccttcactt cttagaaagt ttgctgtatt ttcacatgct ttaaattctt taaatgcttt
    42901 ttctggattt tcccatattt tcttacttaa atcatcaagt ttgaatcttt ttttatctat
    42961 ctcatttata gatatacttc ctatcatttc ctattcctcc tataagtcta tttactatca
    43021 accaaatata attggaccta ttatactagc aaatgccact gatgcaatgg ttacagtaac
    43081 aaatcctcct ataatcatct ttggtaataa ataatctgtt gccctttgtt tttcttcttc
    43141 agttccttcc atagcatcaa taccttcatt agaaattatc tgtgttgcag gataagctag
    43201 catacaagtt acaccaattg cagctgacat tcttggagaa tatcctaata gttttcctac
    43261 aattgcagta aatagtatta taaatacaga gcctacaaca agtattccta tagttggaac
    43321 aatcatccca aacagtgctg ttggagttac cttagcaagt cctccaggta acattaacat
    43381 acagccaaac attataattc cattagattg agatttatct aaaattcctt tttctaaaaa
    43441 acctattcgt gtaaaaatca accctactaa caaacatgct atgttgggat ttaaataata
    43501 gtttgcaggg gtaaatcctg gtattaaagt agccaatcct acaaaattac caatcatagc
    43561 aacaaatgaa agctttgcaa gatacactaa agtagatgat tttttaccta aatcttcttt
    43621 aaaaattctc atacttggaa gtttaaaatt actttttgac tcatctttca aaaaatcccc
    43681 attatctctt ttaaatttta attctttatt tatacaatat gtagttatcg gaatgccaaa
    43741 gaacttttga aatgataata ctagaattac aaatgcagca agttcaggtc tatttgcagc
    43801 ttctgcaaca gttgttacga taatcccagc aacagtagaa cccgctattg gaggtgctgc
    43861 tattaaagca tactccctac caaataacat actacccact gttaaacagc caacccctat
    43921 tcctacaata cctactaatg caattacaac cgttttccat tcatgacata agtcttcaag
    43981 atttataagt gttcctaagt tagttattaa taaagctata ccaaagttgc tcattgcagc
    44041 taatattcct gatgctcctg taatgtcttt tggtaaaata tttgtccaaa atcctagtaa
    44101 aaatataata catacaaata gcaatgttga aattgcacct tttgttttct tagataaaaa
    44161 ctcactaaaa gcataaatca ttacacatac agtaaagcaa gtaagtggat tccaaaaatt
    44221 ttccattgtt gtttcctcct aaatataata attttttatc aataaaacaa taattataaa
    44281 atgtatatta tgcagttatt cttaactaca tatggtatag cttgatattt ttggatgata
    44341 ttatatacgt cttaaattat tcttaaaata tctaaataaa tataactaga gttaactctc
    44401 taaatattaa ctgtagttat caacttacta gatttctttt tgatttttac tttctacata
    44461 attttacaag ttagaattgt acttcaacta ctttctatct tagtatacat gttggaagac
    44521 ttatatttat ttaaatatta gatatagaat acatttttac aaacgtctta aattattctt
    44581 aaaatatcta aaacaaaata ataatatacc cattaaacca atattatcag aatatacttc
    44641 actttaagtc ttctttatgt aaaaatttta tctattagtg catcccctcc tttgcaaatt
    44701 agactatttc taagcaaatt tagatatctt attcctacga actattacta gaaataaaaa
    44761 ttatttatta tattaaatca ataataagcc atactttaaa attgtttcta tacaagtata
    44821 atttatcata taatgacatc tataccttta tctctatagt agtcctaaat tattcttaaa
    44881 tttacaacaa aaataatttc actatttgta aaaatacttg tttttatatt gtcaaactat
    44941 tcttaatata gattataatt tcaaaccttt tctttgtcaa tatattctga catttttcca
    45001 gaaaatttat aaatttatct agtgagtaaa tcatactatt tttatataat gagtaaatca
    45061 tattatgaat atgtctaaat atttacttaa gtataaacgt aaaaaatgtg tattctcctt
    45121 tgtatagtaa ttttagttat cattactttt aagaagaata cacactttat tttaaaaacc
    45181 ctatactcta ttttaaacgc tttactagac actaattaac atcagtttct aacttctatt
    45241 tatcttattc ctactctact actatatctg tagaagaatc taaaacttct ccattagatt
    45301 ttattacctc tttgtaccaa tcaaacgatt ttttcttata tctcttaaaa cttcctttac
    45361 cttcatcatc taagtcaaca tatataaatc catatctctt actcatctga cctgtagttg
    45421 cactgactaa atcaattggt ccccacatca aatatccaaa acattcaaca ttatcctctt
    45481 caattgcaga cttcatttga gatagatgct tacttaaata ttcaattcta taatcatcat
    45541 ttatagttcc atcttcttcg attttatcat atgcccctaa accattttct gtaattaaga
    45601 taggtaattg atactttctg taaatatagt tcaaagtaaa tcttaaacct atagggtcta
    45661 ttgcccatcc ccacttactt tgttctaaat atggattttg aatcccccca aataaagatt
    45721 tatcacctga taaattttct tttggagtag atacacttga aaaataataa ttaagaccta
    45781 taaaatctaa cttaccttct ttaaatgctt ttttgtcttc atcagtcact tcaatattta
    45841 tattattttt ttcatattct tttaatttat attggggaaa cttcccattg cacattgcat
    45901 caatttgata ataatcacgg tcattatcta aaaatgcatt taacacatta ctaggattac
    45961 aattgtatgg ataaattgga tttagtccaa atacacatcc aactttattg ttgggattga
    46021 tttctctagc taactttgca acctttacac ccgccaaagt catattatac cctacatttg
    46081 ccaatgtttg aaccttattt tctatttcac tatacttaat acctgctatc atataagtaa
    46141 aaatatccga atgctctgtt tgagggtcaa tatggttcat ctcattaaat gtaacccagt
    46201 actttacctt atcatcaaac cttcttatta cagtctcact gtactttaaa tacaaatcta
    46261 taagttttct gttattccat gaaccatatt tatgtactaa attcataggt aattcaaaat
    46321 gatatagtgt tacaataggt tctatattat tttctattaa tgtatctatc agaccttcat
    46381 agtatttaat ccctagttca ttaggatttt catcatctcc atttggaaaa attcttgacc
    46441 aatctataga tattcttaat gcactaaatc ccatttcttt aaataaagct atatcttctt
    46501 tgtatctatt ataaaaatct attccattat gagatggata atccaatttt tcttctattt
    46561 tatcagtaat tactcttggc atttcataag agcccttagt tacaaaatcc ataattgcag
    46621 gccctttgtt atcactatcc catgaacctt cacattgatg ggctgcaata cttcctcccc
    46681 agaaaaatgt atctttaata ttcatgaatt tcccctccta aaatttatac tattacagtt
    46741 aataattctt ctgattcatc atttgatgag attacatcta aataatcgct agtatttgta
    46801 actacagtta ttatagttga atcaaaacct tcctctataa ttttatctga ttcaaatact
    46861 gctaaagcat ctcctttttt aaccctttga ttttgtgtta caaaactttc aaagaacttt
    46921 ccatctaggt ttacagtatc tatcccaata tgaattaata tttctaatcc ttcatcagtc
    46981 actataccat atgcatgttt agttggaaat actatagtta tagttccatc tacaggagat
    47041 ataacagttg attttgattc actaggtata actccaaccc ctttacctaa tgctccagag
    47101 gaaaatgctt tatcattaac ttccccaagt gcaattacct gaccttttgc aacatttcca
    47161 atactaatag ttcttgaatt attctcgttg attattagtt ctaattcttt ttcattatca
    47221 tccttaactt cttctttttc atctgaaggt attcctagta aataagaagc tactgttgct
    47281 gctgcaaatg atactactac acctattatc ataaatgtaa agttagatac ctctcctcca
    47341 acaagatatg ttggtaatgc agctaatccc caaaccattc catatgtttt aacttgtgct
    47401 aaacctgcta ttaaaccacc tatacctcca cctatcatta ctgctacaaa tggtcttcta
    47461 tatttcataa acacaccaaa cactgaaggc tctgtaactc ccataagtgc actaaagctt
    47521 actgtaccaa ataattgctt ttgttcttta ttcctagttt ttaagaaata tccaaacatt
    47581 gcaccacaaa ctgcaatatc agatattgtt gcagcgccca taaaaattgg gtcatacccc
    47641 aatgttgtca cagaatttaa agatataggc atagtaaagt ttgctgcacc aaaaactatt
    47701 aagagtggtt gtaaagctgc atacagtgca acaacactcc aattacccaa tgtatcaatt
    47761 aaaaacatac atgcatagtt taatccatca ccaatccaca taccaatagg cccaaatata
    47821 gttaatgtaa ctgataaagt tattattaaa gataatactg ggactaaaaa atagtgtagt
    47881 gctttaggca atattttttt caaacctcca attataattc ccattgacca tactcctaaa
    47941 agtattggaa taaagctgtt tgcataagtt gtaacctgta aatttatacc aaataaactt
    48001 aatccttcta caccatctat tgatgaagat aataacgtaa ctgctaacac tactgctata
    48061 tagggattta cattaagtct ttttgctgat gctattgcaa ttaatactgg taagaagtga
    48121 aatgtagcgc ttcttaatgt atctaatatt tgatatgtag gactatcact ttgaagtatg
    48181 ccagacatat ttaaaagtgc taatatactt gcaagtatac cagctgcaag tagtacttca
    48241 attactgaag tcatagtctc tgagactacc acaaagatgg aattaaatat acctttgata
    48301 cctctaattt ccttttttcc ttcacctttt acacttgatt cacttgaact taaacctatc
    48361 atagacataa actcgttata tacatctaca acatgagtac ctattataat ttgataagca
    48421 acatcattag aaactacatc aattactcct tttaattttt taatttcttc tgtttgagcg
    48481 atactcctat catttaaagt aagtcttaaa cgagtaacac aatgtgctac cgactttata
    48541 ttttcttttc cccctataag ttttattata ttttcattta aatctttata tttcatctga
    48601 ttttgcctcc agtctttgag tcactctatt tatatgcaac attaaataag tttcttcact
    48661 tatagatata tcgacattaa attgtgattt tatatatttt cttatctttt gaacacttcc
    48721 aaatgacttt ggataaagtt gacttacttg tttatataaa tctatagaat tatcttcatc
    48781 tattgaagct tcatctgaca taattctttg gataaagtac tgtagatgag tagtaaatct
    48841 catatagttt gttgaagttt catctagctc tacattaaaa tgatatttta ttatagaaac
    48901 tatatctgct aaagttctta actctacaat cgtactatca tgagactctt tatttacctc
    48961 catatttaca aaatgaagtg ctattgatac tgcttcatca tcaggaagat taattccctt
    49021 actatcacca attaatttta gtgcatgcat accaacttca aaatgttttg gataaaatct
    49081 tttaacattc cataccaaag gactttttat aaattgattc ttttcacatc ttttaagtaa
    49141 aaactctaaa tggtctagta gggttaaact tagataatca cttgacttta tatttaaagc
    49201 tttctcacca tattcaatta tttcattaat gagaactata ttattggagt ctgagttttg
    49261 tagtagataa ctaaagtgtt ctagcatatc gtatgaatct agtatatata ttctctctat
    49321 ttcattttca tttattactt gtcccactct ttttttaaaa ccaattcctt tgacaaatac
    49381 aataatttcg tttttacctc tttttactaa ggctacatta ttatttaaaa tctgaattat
    49441 tttcatatat tcctcctttc aataaaaaga actcaagcat atatgtatag agtatccctc
    49501 tatttttcac atatatactt gagttctgcc tgataatcag taacatactc aaaattatga
    49561 ctacgtcgcc ataattaaaa ttttttttgt ttaatttcat attttatatg ttatactatc
    49621 catgtaaatc tgtcaacggt ttcctagata tttttttatt ctatagctac aagctctgtt
    49681 atgcgattat ctggcaaagt tcatatacta aattgatact attttactgt cactaatatt
    49741 tacaatattg tctgaaccat tatctgtttt actattatga attatcatag taatattttt
    49801 aaatataatg aattgtatgt taataaataa attcttaaga catattaaca tgtaaaatct
    49861 taagtttagt tttatttaat cactattaag atatattaat a
//
LOCUS       DAEMAE010000007        46016 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEMAE010000007 DAEMAE010000000
VERSION     DAEMAE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.007"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1095..1670)
                     /locus_tag="KPH93_000337"
     CDS             complement(1095..1670)
                     /locus_tag="KPH93_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG1687178.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGNDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLSKIDEKTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            1930..3222
                     /locus_tag="KPH93_000338"
     CDS             1930..3222
                     /locus_tag="KPH93_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1687179.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            3327..3929
                     /gene="yedF"
                     /locus_tag="KPH93_000339"
     CDS             3327..3929
                     /gene="yedF"
                     /locus_tag="KPH93_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG1687180.1"
                     /translation="MNIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILEKMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            4002..4265
                     /locus_tag="KPH93_000340"
     CDS             4002..4265
                     /locus_tag="KPH93_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG1687181.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVKNNVDIKGIYCISKLENGSKEVEKLD"
     gene            4280..4465
                     /locus_tag="KPH93_000341"
     CDS             4280..4465
                     /locus_tag="KPH93_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG1687182.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDVKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            4568..5764
                     /locus_tag="KPH93_000342"
     CDS             4568..5764
                     /locus_tag="KPH93_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1687183.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            5978..6256
                     /locus_tag="KPH93_000343"
     CDS             5978..6256
                     /locus_tag="KPH93_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG1687184.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            6293..6727
                     /gene="ssb"
                     /locus_tag="KPH93_000344"
     CDS             6293..6727
                     /gene="ssb"
                     /locus_tag="KPH93_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1687185.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            6746..6973
                     /locus_tag="KPH93_000345"
     CDS             6746..6973
                     /locus_tag="KPH93_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG1687186.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            7116..7436
                     /locus_tag="KPH93_000346"
     CDS             7116..7436
                     /locus_tag="KPH93_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG1687187.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            7444..8400
                     /locus_tag="KPH93_000347"
     CDS             7444..8400
                     /locus_tag="KPH93_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG1687188.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVIV
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            8411..10405
                     /locus_tag="KPH93_000348"
     CDS             8411..10405
                     /locus_tag="KPH93_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG1687189.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            10406..10855
                     /locus_tag="KPH93_000349"
     CDS             10406..10855
                     /locus_tag="KPH93_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG1687190.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            10874..12202
                     /gene="dnaB"
                     /locus_tag="KPH93_000350"
     CDS             10874..12202
                     /gene="dnaB"
                     /locus_tag="KPH93_000350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG1687191.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASIAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            12407..12715
                     /locus_tag="KPH93_000351"
     CDS             12407..12715
                     /locus_tag="KPH93_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687192.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            12797..14086
                     /locus_tag="KPH93_000352"
     CDS             12797..14086
                     /locus_tag="KPH93_000352"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG1687193.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYIAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            14418..14493
                     /locus_tag="KPH93_000353"
     tRNA            14418..14493
                     /locus_tag="KPH93_000353"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14451..14453,aa:Lys,seq:ttt)
     gene            14500..14574
                     /locus_tag="KPH93_000354"
     tRNA            14500..14574
                     /locus_tag="KPH93_000354"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14533..14535,aa:Glu,seq:ttc)
     gene            14579..14654
                     /locus_tag="KPH93_000355"
     tRNA            14579..14654
                     /locus_tag="KPH93_000355"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14612..14614,aa:Val,seq:tac)
     gene            14688..14762
                     /locus_tag="KPH93_000356"
     tRNA            14688..14762
                     /locus_tag="KPH93_000356"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14720..14722,aa:Thr,seq:tgt)
     gene            complement(15146..16138)
                     /locus_tag="KPH93_000357"
     CDS             complement(15146..16138)
                     /locus_tag="KPH93_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687194.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLNVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(16147..17205)
                     /locus_tag="KPH93_000358"
     CDS             complement(16147..17205)
                     /locus_tag="KPH93_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG1687195.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            17330..18793
                     /locus_tag="KPH93_000359"
     CDS             17330..18793
                     /locus_tag="KPH93_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG1687196.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSVGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGIAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            18878..19675
                     /locus_tag="KPH93_000360"
     CDS             18878..19675
                     /locus_tag="KPH93_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1687197.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDID
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            19688..21790
                     /locus_tag="KPH93_000361"
     CDS             19688..21790
                     /locus_tag="KPH93_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG1687198.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSSLFNGEVTNILNFINDSTLGIISLIILLTVSYSYGKIHGSKYTVLVPIVAM
                     CSFLVFSHSNELNSYIEIFKTKWLFTAIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNRMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRTNVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQLFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLVKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGDVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            21858..22337
                     /gene="rlmH"
                     /locus_tag="KPH93_000362"
     CDS             21858..22337
                     /gene="rlmH"
                     /locus_tag="KPH93_000362"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG1687199.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSIKEMEIIKYKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQVYRAYRINNGEPYHK"
     gene            22486..22797
                     /locus_tag="KPH93_000363"
     CDS             22486..22797
                     /locus_tag="KPH93_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1687200.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            22845..23162
                     /locus_tag="KPH93_000364"
     CDS             22845..23162
                     /locus_tag="KPH93_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1687201.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNKTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            23375..24076
                     /locus_tag="KPH93_000365"
     CDS             23375..24076
                     /locus_tag="KPH93_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1687202.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNPQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            24300..25601
                     /locus_tag="KPH93_000366"
     CDS             24300..25601
                     /locus_tag="KPH93_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1687203.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISAALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            25738..26832
                     /locus_tag="KPH93_000367"
     CDS             25738..26832
                     /locus_tag="KPH93_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG1687204.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDTLQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATSTKHLFATG
                     LIDDVIVGNSYASEDELKRMSEVNKYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYSKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            26834..27967
                     /locus_tag="KPH93_000368"
     CDS             26834..27967
                     /locus_tag="KPH93_000368"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG1687205.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDASKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDENELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNDVKKEIIEARERGIILDVGHGTESFSMDVAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDM
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIDKSVEENE"
     gene            27985..29094
                     /locus_tag="KPH93_000369"
     CDS             27985..29094
                     /locus_tag="KPH93_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG1687206.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEIIEAIGEGCKNFF
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKNDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLIIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTLKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKVEIDIRSVIGSDLDIIYNRIKNIVES"
     gene            29144..29899
                     /locus_tag="KPH93_000370"
     CDS             29144..29899
                     /locus_tag="KPH93_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG1687207.1"
                     /translation="MEEINMNCIPQYYKNRVCLNVLAGSIRNAKEVYNACEGHVLVGV
                     LSKNYSTVESAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFT
                     GVATSRALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMG
                     GSSIKFFPMGGLKTKDEYVKVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKV
                     IPHVYTSIIDKETGDTKVEDIEVLYKIMKELLG"
     gene            29904..30896
                     /locus_tag="KPH93_000371"
     CDS             29904..30896
                     /locus_tag="KPH93_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG1687208.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYVITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLESGYGERPSEVT
                     YLNRLNSSFCESKLQDYDINKCLEGMDVVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPEKTYDDLVNDYKKMLPYCHGLFASYRDINKILNIDNLDIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMDNRFIKGYISNDDGYFESKEYKLEIYDRVGAGDGYVA
                     GIIYSYLENMSNEDIVEFGVASSVLAHTTYGDNSLVSTSQVLRLMNNEKIDILR"
     gene            complement(31083..31964)
                     /locus_tag="KPH93_000372"
     CDS             complement(31083..31964)
                     /locus_tag="KPH93_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1687209.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVASNMGISILPSFCITDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            32237..33625
                     /locus_tag="KPH93_000373"
     CDS             32237..33625
                     /locus_tag="KPH93_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687210.1"
                     /translation="MEDKFYAKGKGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRIFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKIKED"
     gene            33653..34606
                     /locus_tag="KPH93_000374"
     CDS             33653..34606
                     /locus_tag="KPH93_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1687211.1"
                     /translation="MKILGISFGTKNGNNDTMCKIALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSICVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICSLCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGISLG"
     gene            34666..34890
                     /locus_tag="KPH93_000375"
     CDS             34666..34890
                     /locus_tag="KPH93_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687212.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIMQVREATNYNIGNG"
     gene            34883..35395
                     /locus_tag="KPH93_000376"
     CDS             34883..35395
                     /locus_tag="KPH93_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG1687213.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLALYMGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIGVAMSVGVWYVGAGVDLFTRY
                     ILSKDKKKRN"
     gene            35428..36282
                     /locus_tag="KPH93_000377"
     CDS             35428..36282
                     /locus_tag="KPH93_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG1687214.1"
                     /translation="MVMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGVRKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            36816..37610
                     /locus_tag="KPH93_000378"
     CDS             36816..37610
                     /locus_tag="KPH93_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1687215.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNNFD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGRIKHVTDYDEYGALKENTNNLKKAVTLTDL
                     RRVISFNEEGIIEEKFIIVKEVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIKNEYELLGIVYVNILLTTYEEEIERIFLEIYAPIK"
     gene            37714..38520
                     /locus_tag="KPH93_000379"
     CDS             37714..38520
                     /locus_tag="KPH93_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1687216.1"
                     /translation="MGTEKSLKRPVASFIILTNIIFLPLFLLVVVTIILKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRYSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            38582..39103
                     /locus_tag="KPH93_000380"
     CDS             38582..39103
                     /locus_tag="KPH93_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG1687217.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            39518..41605
                     /locus_tag="KPH93_000381"
     CDS             39518..41605
                     /locus_tag="KPH93_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1687218.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNAENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDYIKTHEL"
     gene            41620..41907
                     /locus_tag="KPH93_000382"
     CDS             41620..41907
                     /locus_tag="KPH93_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1687219.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTELEKFLASKEG"
     gene            41934..43205
                     /locus_tag="KPH93_000383"
     CDS             41934..43205
                     /locus_tag="KPH93_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG1687220.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAIEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGKVFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            43263..43862
                     /locus_tag="KPH93_000384"
     CDS             43263..43862
                     /locus_tag="KPH93_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1687221.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            43867..44772
                     /gene="dapA"
                     /locus_tag="KPH93_000385"
     CDS             43867..44772
                     /gene="dapA"
                     /locus_tag="KPH93_000385"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG1687222.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPCFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            44878..45510
                     /locus_tag="KPH93_000386"
     CDS             44878..45510
                     /locus_tag="KPH93_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG1687223.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTINNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAVLHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGTVEDITENAKKIREIIKNIREV"
BASE COUNT        17856 a         5033 c         8146 g        14981 t
ORIGIN      
        1 ttactgaata tttttatttc ttatttttta gaggaatttc ttttaattta aatataaaaa
       61 ataataaatg tgtttatata gtaactgcat atattactag tttaaaagga cactattcat
      121 tcgtagaaac tcaaattgat gattttatta aaaaatatgt agaagatatt gtagatttat
      181 gtcttcatag aataatgatt ctattatatg aatgtgactt aacataaaat tagatatatt
      241 aaagttgagt gagaaaaatc ttgaataaag ttcttcttac caactttaat atatttctat
      301 tatacaatct attttttatc attattgaaa ttgtttacaa cttgatttaa ttcaattaat
      361 atacttccaa cttccatata gagaatctta ctataaacac aagtaataca ataggaacgt
      421 aattatcact tgattttttt attttttaaa tcccataaca aaacattaag tgcaactatt
      481 ggagttaaca attgttgcag tataaaagct attaagttat ctatatatta tcttcctgaa
      541 aatattttct taacacttta attattattc aaaaactatt taaacaaagc tataagagag
      601 tttaagttat tactccaata agatttattc tttattttat acatttattt tttcaaaaac
      661 tataaaagaa cagtatattt tttagacgta gaatttataa ctaaagatta taatattgct
      721 attgaagcta agcattataa aaaacattgg atacaattta ttgatatagc attttcaaaa
      781 tgtcaatatc acttgttata ttactgtaag ttgtatagat tatgttagcc ttgaaagtat
      841 ttgtgtatat atggtgacta ttataactga caatctggta aataagataa agataacgtt
      901 agtagggtaa aatattgctc tataaaagat ataaatagaa tatgaaaggt gttatttcaa
      961 aattgatttt ttataatcaa atttttattt ctatttttct gtttttatat gtaaaaaaga
     1021 aaggaaggag atgattcaaa tttttatttt gaatcatctc cttagatatg ataatttata
     1081 acagtttcaa gaaattactt taagtaatct ccttcttcaa gaactatatg gccactttcg
     1141 taattagaac catcaacagt aatttttatt tgttcaacat tcatattttt tatgtaagta
     1201 cgagcaactg aatcaagcat taaagtttct gcatcactac ctaagtttga ctttgtaaag
     1261 tcagagctaa catctaatat cccagttttt acgccatcaa tgtcttttgt agtaaatgaa
     1321 tttaattttg cagattctgg aacaacttta agtttttgaa gttcttcaaa taaagtcttc
     1381 tcatcaattt tacttaaatc aactttattt tcaattaaat ctgtcttatc tacatcaaaa
     1441 gagtaaatag tagctgattg agttttagtt tcttcttttg gagtaggttt agctttgtta
     1501 ttaggaacct ctttttcagt attgttttct tcttttggtt gttcaacatt tgtattgctc
     1561 ttagtttctt ttttagatgt atcatttcca ttttgagcat tagtacaacc aactgcgaat
     1621 atagatatag ataatatact taacactaac attattttct tatttttcat aaaaatttct
     1681 cccctcttaa aaactattct tatatataat tatagtcgtt atgagcaaat atatgaatac
     1741 aaaatagtaa caaatttaaa aaatgattta tttaaattaa taaaactcaa aataaactaa
     1801 attttagtca tttttatggg aaatcattta atgtacactg aaaatattgt aaaataaaag
     1861 tagtgttttt aaaataaata taagttataa ttatattata aaaaaatgtc ttcaaaaggt
     1921 gggaatagta tggaaatttt gagcttagga gaaaagatta aaaaattaag aaaagaaaaa
     1981 aacatgacat taaaagaatt agctggagat aggataacag cagcacaaat aagccatata
     2041 gagagggata aatctcatac tagttatgaa ctgttggaat atctaggcga taagctagat
     2101 gtaagtgtag aatacttatt agagaccaag gaaatgcaat caaaaaagat aactgataat
     2161 ttaatactac agagcgaaat ttatataaaa gaaaatgaat taaacaaggc tgaggaccaa
     2221 ataaaagaag ttttagatat atgtaagaga tacgatttaa tggagaacta tggcagatgt
     2281 aattttttaa tggctaatat taatttgaaa aaatctaatt ataatgatgc tatagaaaac
     2341 tttgaaaagg cattgtactt ctttataaaa aatagtgata gtgaaaatat acttaagtgt
     2401 tatttaaaca tagggaaaat atatatgcag gaagactttt ataaaggtgc tataagccac
     2461 tttaattttg cagaggaaat tttgtcagag aataaatttg aggatgttaa catacacaag
     2521 gaactatata gtaagatggc atattgttac ataaaactag ataatccaaa tatgtcattg
     2581 aagtacatag aaaagataaa tgaaatagat aatagaaatg atagaaaaga agatgtcgat
     2641 attttggtgt taaaagcaaa taatatgctt gagattggaa aatatgaaga atcaagagat
     2701 tactttaaaa aagcactaaa aatacttgag agtgaggata ataaaactgg attagcaaat
     2761 gtgtatttaa caatttgtga tgtttacaga aaaataggtg aaacagatag agtacttgaa
     2821 tattcacaaa aggtatatga cataaagaaa aatgatgaag atgaatacat gatgtcagga
     2881 ttatttaaga taatagaagc atatatagat aaagaagatt atgatttagc tagaaaatat
     2941 tgtaagatag ccttagcttc atcaataaaa aataaaaata aattcaatga atataaagca
     3001 ttgagattct atgctaatat gtataaaaat cagaatgaag taactcttgc aattgaatat
     3061 ctgattaaat gcataaaaat agtatcagat ttaggtaaca atagaatatt ggccaattta
     3121 tatatagact taggtcaatt atattcaaat atatcaaaag aaaaagagtt agaatattat
     3181 caaaaaggtg tatttatgta caaaaattta gaaataatgt agggtaaata aacctataag
     3241 ggtatagaaa caatctataa caagcaagat aatatctatg ataaaatata aattgtatga
     3301 atatttttaa ggagatgaat cagacaatga atataaaaat agatgcaaga gggcttgctt
     3361 gtcctaaacc agttataaac acaaaaagag aattggataa cctagaagaa ggtgttgtag
     3421 ttacaatagt tgacaatgaa acagctaagg agaatatatt aaaacttgct aaatctttaa
     3481 gttgtgaagc aaatattgtt gacgaaaaag agaattttat atctatagaa attagaaaag
     3541 gtaaaaatat actagataaa caaaatgttg tagataataa agaaagtgaa ttagacaata
     3601 catgtatatt tatatcttca gataagatgg gacttggaaa tgacgaatta ggaaaagtat
     3661 taataaaagg atttatatat acattaacag aatctaaacc ttatcctaag tatgtacttt
     3721 tagttaatgg aggagtaaaa ttaagtgctg aaaatgaaga aactatagaa aatctaaaaa
     3781 tacttgagaa gatgggtgta gaagttttat cttgtggtac ttgtttagat tattataatt
     3841 taaaagacaa actgcaagtg ggtacggtta caaatatgta tactatagtt gagacattaa
     3901 agaatgcatc aaataccatt tctatataat gtatgtttgt ttaataatat aaaactaata
     3961 ataaagatta actcaaatag atagtcagaa aggattaagt aatgaatcaa atgtatatag
     4021 tgtcatttaa ttcgacacat catgcgataa gatcagaaaa gttatttggt gaaaatagtc
     4081 taaaagtaat ggctcttcca acaccaagag aaataacagc aagttgtgga atatcaatta
     4141 aattttcttt tgaagatatg gaaaagataa aaactatttt ggttaaaaat aatgtagata
     4201 taaaaggaat ttattgtata agtaaattag aaaatggttc aaaagaagta gagaagttag
     4261 attaggaggc taattattta tgccaatgga tttaaaaata ggtgatattg ttgaattaaa
     4321 aaagcaacat gcatgtgggt gtaaagagtt tgaaatcata agaacgggaa tggatgtaaa
     4381 gataaaatgt actaaatgtt caaggctaat aatgcttgat agagagacct tagaaaagag
     4441 agtaaaaaag ctaataaaaa agtgataaaa atttaacact taaaaaatgt caaataccgt
     4501 atattaatat ccaaaatttt ggtataataa gaattgaaga aaacattttc atgggggtgc
     4561 tttaattatg gcagttaaat atgcaaaaag aatgcaagga ttacaaggat cagaaatacg
     4621 tgagctttta aaacttactc aacaaccaca aataatatct tttgctggtg gaatgccggc
     4681 tccagagtta tttccagttg aggaaatgaa aaaagtatca gttgcagtgc ttgaagaaaa
     4741 tggaagatca gctatgcaat atactacaac agaaggatat gaaccattaa gagaaaaaat
     4801 agcagctaga atgaatgata aaaacaaaac taatgtaaac aaagatgata tattagttac
     4861 aagtggttct caacaaggtc tagattttgc aggaaaagta tttattgatg agggtgatgt
     4921 aattttatgt gaaagtccat cttacatagg agctataaac gcatttaaat cttatcaacc
     4981 taaattcata gatgttccaa cagattctga tggaatgata atggaagaat tagaaaaaat
     5041 actagaaaca actgatagaa taaaaatgat ttacgtaata cctgacttcc aaaatccaac
     5101 aggaagaact tggccacttg aaagacgtaa aaaattcatg gaaatagtaa acaaatttga
     5161 aataccagta atagaagata atccatatgg agatttaaga tttgaaggag agactttacc
     5221 atctttaaaa tcaatggata caaaaggatt agtaatattc ttaggaactt tctctaaaat
     5281 attctgccca ggatacagat taggatggac ttgtgcttct ccagaaattt tatctaaatt
     5341 taactttgca aaacaaggtg cagacttaca agcatcaact atatctcaaa tggaagtaag
     5401 caaatttatg gatatgtatg acttagatgc acatgttgac aaaataaaag cagtttatgt
     5461 taaacgtaga gatgtaatgc ttaagactat ggaagaagaa ttcccagaag gattagtatt
     5521 tactcatcca gaaggtggat tatttacatg ggtagagctt ccaagtaatt taaatgctaa
     5581 agaattaatg ccaaaatgct tagataagaa tgttgcttat gttgctggag gaggattctt
     5641 ccctaatggt ggtagagaaa atactttcag acttaactat tcaaatatgc cagaagaaaa
     5701 aataatagaa ggtataaaaa atatagcagc tgtattaaaa gaagctatgg gtgtagaagc
     5761 ttaattaaaa ataataactt atatataata caagggatgg ctgttgccat cccttgtttg
     5821 tttgaaaaat atataattca ccttatacta aactttgtcc aaaatttaaa ttgacagaaa
     5881 taagaataaa tgataaaatg tgtatgtatg agattttagt ctcatttctc tgctctaaga
     5941 gtgtagagcc gttaagtcca aagggaggtg aattatagtg agaaattatg aattagttta
     6001 tgtggtaaag ccaaactctg atgaagaagt aagagaggct atactaaaca aagttaagga
     6061 agttgttgca actgacggag aaatagttaa agttgatact tggggaacta aaaaattagc
     6121 ttatccaata gctaagttta cagaaggttt ctatgtatta gttaacttca aatcagcagt
     6181 agacgttcct aaagagatag acagaaactt aaagataaat gaaaacgtaa taagacacat
     6241 gatagttgtt gcttaataac tggttttatt ataaaaaata ggtggtgttt ttatgaatca
     6301 agttgtatta gtgggaaggt tgactagaga ccctgaatta agatacatcc caggtacagg
     6361 aacaccagtg gcatccttta caatagccat tgatagagac tatgtaaaaa aagatggatc
     6421 taaagaaact gattttatac ctgttgaagt aatgggtaaa tctgctgaat tttgtgctaa
     6481 ctacataact aaaggtagat tagtagcact tcaaggatct ataagagtag acaattatca
     6541 gacacaatca ggtgaaaaga gaacttttac aaaagtaagt actagatcag tacaagcttt
     6601 ggatagtaag aataaatcgg aaaattcata tagagaaagt gctccagctt ttgagccaag
     6661 ctttgaacct caaggtttag acccacaagg tttccaagct atagatgatg acgatatacc
     6721 attttaaaaa ggagggaaaa aagtcatgat gaataagaaa agacgtaaga aaaaaagagt
     6781 ttgtcaattt tgtgctgaca aaaacgctaa aatagattac aaaagtactc aaagattaca
     6841 aaagtatata actgaaagag gaaagatatt accaagaaga atatctggaa cttgtgctaa
     6901 gcatcaaaga gaattaacag ttgctataaa aagagcgaga aatatagcac ttttaccata
     6961 tacattagac taaaaaaatg agccttgtgc tcatttttta ttatatgtat ttatatatct
     7021 aaatgaggcg agctataaag ctcgtttttt tgtttatata cttaaatata ttattattta
     7081 tgatataata ttatatatta aggagggttg taaacgtgaa aatagataaa ggttctgaaa
     7141 taacgagaaa tataaagata atagaatgga tgaaaacaga aatattaatg agtgtaagtg
     7201 atttgtttaa tttattgttt aaaggggtaa aaccattaga tgaagcaatt caagatactt
     7261 tagcaaatat aataatgata acatatctct tagcaaagag attaggaatt agttttagag
     7321 atgtggatta taaagtcaag gaaaaaataa aaatcggtat agatgaggac catagcgttg
     7381 aaagatggta tggagattta tctaatttaa aaaaacatat ggataatagg gagtgatatt
     7441 aaattgaata ataaaattag actatctaag gcaatgtcta taatagtttt agctataata
     7501 atcgctttag taatagccta tattccaatg ctaggaatat ttagtatgtt agcagctgtt
     7561 ccatatgtaa ttgtaggtgc tattactgat agaaagtatt ctcttatatc tattttggtt
     7621 acattttgtg tactgattct atttgcagac atttcttatg cattaaatat atgtataatg
     7681 tattctttac caggtatagt aataggaaag atgctaaaga gaagctttga acaagaggac
     7741 agtaataaat tcgaaccgat atacggtgga accataatat ttgtactttc aatgcttgta
     7801 tatttttttg tattaaagac atttttaaat gtgaatctat tagatgaagt ttctaagatg
     7861 atatcagaaa ttgtaaatat tcaaaaaaat agtttttctg caactgaact taaagtgttt
     7921 gataaaatga agccagatga gattgtaagt tattttacaa atatgctacc aatgatgtta
     7981 tttttacaag ggttgttatc agcctttgtc acatattatt tgagtgtatt ttttataaaa
     8041 aggattacaa agatgaatat aagatttcct aaatttgctg atttttattt accgggaaat
     8101 gctatactca caactttctt actttattta ttagtcttat ttattcaaat tattggttca
     8161 aaactatata cagaattaat aatgactaat ttacagctag tttttaattt aatgtttgta
     8221 attcaaggta tagcagtttg catttatttc ttaaagaaat ggataagaca gggaccaaat
     8281 aaaattatgt ttttttcagg gataatttta tgtttatttg gatttatggg aatatctttt
     8341 gttggtatgg tagatagtat aattgacttt agaaaggtaa gaagctataa atccacttag
     8401 gaggatagaa atgagtaata aacaaacctt taaattaaat atgccagaaa taaatttata
     8461 tataattgtt attggtattt ctagtataat tttgctttat tacaacttgt atgtagggtg
     8521 tttatttttc tgtatttttg tatatatggt ttttcacaat tggagaacta ctaatatcag
     8581 acgtcatgaa tggacagagt atattcaaaa tttatcttta gatatagatg aaacaactaa
     8641 aaaagccata ataaatcttc caataccatt atgtatttta gaatttgatg gaaatatatc
     8701 ctggtataat ggaaagtttt atgatatgat tggtcaaaaa gatttacttg ataaaaatat
     8761 agaggatata gttaaaaatc ttaatttaag aaaagtatta aatgaaaata aagagatgta
     8821 cacagaaata aactataaag aaaaagaata tacaataatt tataatgtta taaaaaatga
     8881 tcaagaaaaa aatcctaaat atttgatgat attatactgg atagacaaga ctgaatattt
     8941 aaaagtaaaa caaaattatg atgatgaaaa aaatgcaatg atgctaatac aagtagatgg
     9001 atacgatgaa gtattaaaaa gtgcagcaga agataagaga gctttaatta atgttgaagt
     9061 agagaaaata ctttctgctt tagaattaaa ttcaaatggt gcattgagaa gaacttcaaa
     9121 agacaaattc ttcttggtaa tgcataaaaa agagttaaaa aaattggaag ctgagaagtt
     9181 ttctatttta gatacaataa gacatattga ttatggaaac aatcttcctg ttactataag
     9241 tataggaatt ggtatagatg gagatacatt aaatgagaac ctaaaacttg ctacaggtgc
     9301 tctcgattta gcacttggta gaggtggtga ccaggctgtt gtaaaaacaa aagataaatt
     9361 tgtgttttat ggaggaaaat ctaaagctgt agaaaagaaa accaaagtaa aatccagatt
     9421 aataggtcat gctttaagag aagttataca acaaagtgac caagtttata taatgggaca
     9481 taaatatcct gatatggatg ctatgggagc agcggttggt gtttatgata tatgtaaatc
     9541 ctgtaacaaa actgcaaaca tagttttaca atctgtaaat gaatctatag aaatatttat
     9601 aaataaaata aatgaaaata attactataa aaagttgttt attggaaaag aggaagctat
     9661 tgacaactgc actaaaaata cattagttgt agtagttgat actcataggc caaattatac
     9721 tgaatgtgaa gaactgttaa agctatcaga gaaggtagta gttatagacc atcatagaag
     9781 aggtgtggag tttataaatg atgcagtgct tttattccat gagatatatg tatcctcaac
     9841 gtgtgaaatg gttacagagt tagttcagta tatggatgaa gatgtaacaa taaataaact
     9901 tacagctgaa ggacttttag caggtataag tctagatact aaaaactttg cattcaaaac
     9961 aggtgttagg acttttgaag ctgcttctta ccttagaaaa gttggagcag atactataga
    10021 ggtcaagaaa ttcttcaatt ctgatgtaaa agatttcata ataaaagctg aaataattca
    10081 aagtactaaa ataattaata atagaatatg tttagcatat tcaagtactg agatagatag
    10141 tataaatgtt ataatagctc aaactgctga tgagttatta aatatcaaag aagtagaagc
    10201 ttcctttgta ttaggtgaaa aagatgatac tatatttata agtgcgaggt ctttaggaca
    10261 aataaatgta catgtactta tggaaaaatt aggaggcggg ggacatatag atatagctgg
    10321 agcacagtta aaaaatgttt ctctaaaaga agcttataag atggtaaata aaataataga
    10381 ggagtatttg gaggaggaag aatagatgaa agttatatta ttaaaagacg taaaaggaac
    10441 tggtaaaaag ggagaaatga aagaagtaag tgatggatat gcaagaaatt tcttatttcc
    10501 taaaaagatg gctgtgcaag cagatagtgt agcaataaaa gaattaaatg aaaagaataa
    10561 atctaaagaa ataaaagcac agaaagagta tgaagaagca gttttattag gtaagcaaat
    10621 ggaagaaatt aatatagaaa tatattcaaa agcaggggaa ggtggaagat tatttggttc
    10681 aataacttct aaagaaattg ctgagcaatt aaagaaacaa aaagacatag acgtagataa
    10741 aagaaaaata ttattagatg aaccaataag atctttaggt tcaacttttg tagaaataaa
    10801 aatacatcaa aaagtaacta ctaaaataag agtggatgta aaagaaaaac agtaataact
    10861 agaggtgatt aagatggaag atatgacgag aattcctcct catagtgtag aatcagaaca
    10921 atcaatattg ggttctatac ttctagataa agatgctata ataacagtta cagaaactat
    10981 aaagcctgat gatttctata aagaggctca caaaataata tatgaatgta tgataacttt
    11041 aagtaataaa ggtgagccta tagacttgat aacattaaca gaagaactaa gaaaacaagg
    11101 gcatcttaat gatattggag gaatcagtta tattacaagt ctttctacta tagtaccgac
    11161 gacttcaaat gtaaaatact atgctgatat agtaaaagaa aagtctgtac ttagaaagct
    11221 tataaaagct tctaatgaaa taataaactt aggatatagt ggagcaacta agattgaaga
    11281 tgttttagaa caagctgaaa agagtatatt tgatatatca caggagaaaa ctagtgatga
    11341 ttttaaatct ataaacttag ttttgatgga tgcctatgat atgatagaaa aattatatac
    11401 taacaagagt gatgtgactg gtataactac tggttttaaa gatttaaaca aaaaaataaa
    11461 tggtcttcaa agaactgatt taatattaat tgcagctaga ccagcaatgg gtaaaactgc
    11521 cttttcttta aacttggttc aaaatgcagc cttaaaaggt gatgcctcta tagctgtatt
    11581 tagtttagag atgtctaagg aacaattagt acaacgtatg ttatcttcac aatctagtgt
    11641 agaattaaaa aaaataaaga ctggaacact taatgataat gattggccta gaatcataga
    11701 tgctatggca gtattatcag atgccaaaat acacatagat gatactcctg gtataaaaat
    11761 atcagagcta aggtcaaagt gtagaaagtt aaaaatagaa aaaggactag atttagtgct
    11821 tattgattat cttcaactta tggaaggtga aggtaataat gaaagtagac aacaagagat
    11881 atctaagata tcaaggtcac taaaaatatt agcaaaagaa ttaaactgtc ctgtagtagc
    11941 actatctcag ttatcacgtg caccagaaca aagagcagac catagaccta tgttatctga
    12001 cttaagagag tctggagcaa tagaacagga tgcagatata gttatgttct tatacaggga
    12061 cgaatattat catgctgatt ctgagagtaa aaatatagga gaagtaataa tagcaaaaaa
    12121 tagacacggt gaaactggtt ctgtggaact tgtttggctt ggtgaagttc aaagatttgg
    12181 ggataagttg agagacctat aatctaacga atatccacag aacgtccttt ctttctacag
    12241 gaaaaatgtg gatacatgtc actaaatatg tccaaacatt gaaatttagc taaaaaatta
    12301 tccacactat ccacagatgg cctgttgata agtgtgaata agtagataaa gcaagctttt
    12361 ggcttgcttt atctgtgtat aaatattaat tattaggagg aataatatgg aaaaaataag
    12421 cttaaaatat atatatccca atataattaa agtacttgat gaaataaact tatttagagt
    12481 aattgacaat aatttaagag aatctattgt tgtttatgct aataatgtgg ataatcagta
    12541 tcatataaac atgactaata caaactttgg aaatataatt aacatatgta aattagaaaa
    12601 acttttagat gtggataaat ttatggaaaa agttataaaa tatgaaaaag aaataataga
    12661 gaaagaagag ttttcaaaaa tagaagaata tatgttaaat attggagaat actaaaaaag
    12721 ttgaaaaaaa tacgaatgtt tgatagaata agtaagtaaa gtgttcgaga gtatataaaa
    12781 tatagaggtg aattacatga aaacagttgc aattgttggt tctcaatggg gagatgaagg
    12841 taaaggtaaa gttatagatt atcttgctac tcaagcagat gtagtagtaa gaggacaagg
    12901 tggaaataat gcaggacata cattagttgt agaaggtaaa aaatacgcat tacatctgat
    12961 tccatctgga gttttaaatc caaatacagt gaatataatc ggaaatggaa tagtgtttga
    13021 ccctaaagga tttttagaag aattggaaat gtttaaaacg gataatataa gtacagaaaa
    13081 cataaaaata agtgatagag cacatgtcat attcccatat cataaagaac tagatgcatt
    13141 gtcagaagaa gccagaggcg atttaaagat aggtacaaca aaaaagggta ttggaccttg
    13201 ttatatggat aaaacagaga gatctggtat tagaatatgt gatttaatgg ataaagataa
    13261 atttgctata aaattaaaag ctcaaataga tgctaaaaat gaaatagtta aaaatatata
    13321 tggaaaagaa gaattatttg attttgaaac tatatataac gaatatctag gatatgcaga
    13381 acaaataaga aaatatgtgg cagatacgtc tgttattgta tatgatgcag taagagcagg
    13441 taaaaaagta ttgtttgaag gagcacaagg gactttatta gatttagatt taggaactta
    13501 tccatttgtt acatcttctc atccaacatc tggtggattt gcaattggtg caggtatagg
    13561 accaaatatg ataaaagacg ttgttggtat agtaaaagca tatactacaa gagttggaga
    13621 aggaccgttt gttacagagc aaataaatga aactggagat aagataagag aacaaggaca
    13681 tgaatttggg gtaactactg gtagaccaag aagatgtggg tggtttgatg cagttatagt
    13741 taagtatgca gctagagtta atggcttgac tagtatttca tttatgttac ttgatgtatt
    13801 aactggattt gataagataa aagtttgtac gtcttataaa atgggcgata aaataataac
    13861 tgatttccca gcttcattag atgatttagc aaaatgtgaa cctgtatatg aagaattaga
    13921 tggatggaat gaagatataa ctcaaataga taactttgat gaccttccag aaaatgcaaa
    13981 gaaatatata gcaaaaatag aagaactagt tggagtaagt gtagatatgg tttctgttgg
    14041 tccaaataga gctcaaacta taataagaag aaatatattt gcttaatatt ttatcaatta
    14101 aataatttaa atagtaaata gttgaagaag agtatctcaa gataatgttt atagttatta
    14161 gagatgctct tttatgtttt acttacattg attttttatt aagttaaaac tactgataca
    14221 cattctaact gaaattaaag ttgaatttgt gtagatacat ataggttttt taaaaagaat
    14281 tttgttgaaa ataaatagct aaagatgatt ttaattatat gctaaaaaaa gtaaaaaaaa
    14341 ctattgacta aaaattgtat agacgctata atataacttg tgagtgaaag aaacgacgaa
    14401 aaaagtcgaa aaaatgtggt ctattagctc agtcggtaga gcacctgact tttaatcagg
    14461 gtgtcccgcg ttcgagtcgc ggatagatca ccaaaatatg gctcgttggt caagaggtta
    14521 agacaccgcc ctttcacggc ggtaacaggg gttcaattcc cctacgagtc accagtatgg
    14581 gactatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag gttcgagccc
    14641 tgttagtccc accaaatttt gaattagaga taaatactag attatatgct ggtgtggctc
    14701 aacggtagag cagctgactt gtaatcagca ggttgtaggt tcgattccta tcaccagctc
    14761 cacaaagtac ataaattcaa attaatataa ttttctatgt tacaggatac ttttaagtat
    14821 ctttttttaa tttcattttt tattatatta aaaatgaaat ttaacataca acaaaatttt
    14881 gaattacttg caatgtgata gttttaaata taaaatttag aatcattcta gtatagtatt
    14941 atgtaatata gcaaatttaa tttatggtat caataaatct agactactga gaatgtaaat
    15001 gatttgttag gttaatatac attagatatg taaatagaaa aataaaaagt atggtttaat
    15061 ttatgtggca taagttttaa acagagaagc aagtttgctc ttacctctct atttaaaaat
    15121 aaaaaaatgt tttttaaggg ctattttatt tgaatctttc ccatctcaaa tcatctccag
    15181 taaattctaa aatcctaaac ttatctaaga ttctagagag agttctttga gtgtaagtat
    15241 ttcctatttg agacggtgtt aagttggttg aaataatgat ttttttacca gcaacaagtc
    15301 ttgtatttac aatattgaag atttctccac tagtaaaaga attattaagt tcagttccaa
    15361 ggtcatctat tattaataaa tcacaatcaa acaagttttt ataattatct tcacttattt
    15421 ggttatttgt gtctcttcta aatttataat cttcaagaat atctaaaatt ctaaaagaag
    15481 tttgatagat tacaacattg cctttatcta acaattcctt tgcaatacaa ttgcacatat
    15541 aggtctttcc tagtccagta gaaccataaa acagtagatt ttcactatta tctttcttaa
    15601 aatcatgaac aaaattttcg cagatactta aattatttag catattttct ctaggagata
    15661 tttctccatc agagcctttc ttaggagaaa atatattcaa gttaaaattc gaaaaatttt
    15721 cctgacttaa aatcctatct aaattagaca ttttataagc ttcatttaca atttcttgtt
    15781 tgaggcaaga acatttttct ccattaggta aaaatccttt atctttacaa gaaatacatt
    15841 gaaactttat ttccaagtaa tcaagaggta tgttactttc agctaaaagt ctttctttct
    15901 ttacatttaa agactctata ttctctttag tcttctttac aatttcatct ttagatttgg
    15961 gatttaaaag tactatttta gctaaactga gacctatttt agaaatttca tcatctatag
    16021 attttatctc agggattcta ttatatacct cattttttct atgttctaat aagagttcat
    16081 tgtcatctct tctcttagca tatttagcaa gtatttttct tattttatcc tcattcattt
    16141 aatcacctac ttaaactttt ctttttgact ttttttaata atctcatcga gctcatcaga
    16201 ggtatactga gtgaatgttt cgttgaaatt atgaaactta gttttcttgt aagtattgtt
    16261 gttttgagta gtattaatct gcttcttatt aatattctcc ttaactatac gttcttcctc
    16321 tttttgcttt aaatcattta aattttttat atttttttca ttccaatttt taattatacc
    16381 atttatataa gatatacttg ggtttgaaat attcttagat ttagaacatg catttattat
    16441 caaatctata tccatattaa atttatcaaa ccaggtgtcc ataagttctt tttctgattt
    16501 tgatggttgt cttgaaaatc ccaattcatt gaaaatcgtt ttatataaaa tatatcgttc
    16561 acttcttacc aaaaagcttt cttcaacatc ttttggtgta tataaatttg aatcatacca
    16621 atttctaata atgccttcaa tatatttaac tggctttgat gtaccagttt tatctttgac
    16681 gtattcatat gcgcaaagta tcatatcggg gctcatattg tatttattca ttatatccat
    16741 aatactcatc ttttcacttg ggtcgagata tcgcccaact attttattta tagaattaaa
    16801 catttttcta atacttggat tttcagaagt agatacaacc ttatctgtat tagattttat
    16861 agatgaatta ttgcttaata tattctcaat atagaaattt tttaaatcta aaaattctat
    16921 tgaataatta aaattatcat attctccatt gtcatgtatt tttataatct tcttttcttc
    16981 ccagaatttc caagcagata acacatcaga caatggtata tttaggtttt tggctattga
    17041 attattgtcg aacttaggat tagaagtaat atcacatgct tgtctatatc ctaaaagata
    17101 aacttttacg tataacccat ccgccattgg cataaatata tctataaaga tattgggaat
    17161 agtgatttca cctaaatcta tttcattgct ttctttaaaa aacattttat cacctcagat
    17221 taaattatat cattttttat attatatttg tctgtaaaca aataaaaggt cacaagatta
    17281 gcatgtaaat attttctaaa agtattcatt ttgaaaagga ggagatattt tggttataaa
    17341 attcaaccaa aaagtaagaa gaacattatc agtagcgatt ctatttatgg tattaataat
    17401 aggttgcttt atagaaaagg atgacagttt agaaacatac aaggaaaaaa ctaattcaaa
    17461 aataaatcaa tataatatgg atgttatatt tgatgatgag actaagaggt taatgtgcaa
    17521 tcagaatgtt gactacataa ataatacaaa atcaaacata gataaaatat atttccatat
    17581 atatccaaat gctttttcca agaaagattt tgctcctttt gaaaaaagtg aaatgacaag
    17641 ggcttatcct aatggattta acgaaggata tatagatata aagaatatat taaataataa
    17701 tagtaaaatg gagtataaaa ttaaaggaga caaaaatgac atattagaaa tagaattagg
    17761 gaaagagtta aaaccaaatg aaagaatgtc attagatata aaatataacg taaaaatacc
    17821 aaattcagtt ggaagatttg gatatggaga aaatactata aatgttacta attggtttcc
    17881 tatagcttgt gtaaatgatg aaaggggatg gaatttaaag agttatgaaa ctattggcga
    17941 cccattttat agtgagacta gcgattttca tgtgaaacta ttgattccaa ataaatataa
    18001 aattgctcat acaggaaaac ttattgatga taaacatgat aataagaaaa tgctatatga
    18061 aatagaagct gaaaacgtta gagattttgc atttatacta agtagtaaat ttgatgtaaa
    18121 taaagtagat tcaaagggaa tagctatcaa tacatataat ttaaataaaa aactttctaa
    18181 aaaagcaact gaagtggcaa aagattcaat aaatatattt agtgaattat ttggaaagta
    18241 tccttacaaa gaattttctg ttgtagcaag tgactttttt atagggggga tggaataccc
    18301 tatgcttgtt atgatagacc aaagtttata taatgaaaaa aatgaatttt tactagaata
    18361 tgttattgcc catgagacag cacatcaatg gtggtattct gctgttggaa atgatgaaat
    18421 aagtgaaccc tggttagatg aagcacttac tgaatattct acaatagttt attttgaaca
    18481 aaagtatggt aaagatatgg caaacaagct tataaaaact atggagatac agacaaaaag
    18541 ttatttgagc gaaaatatat ttaagccagc aaatgagtat aaaaattcta ctgaatacag
    18601 tttgaatgtt tatacaaaag gcgcaatagc atttaatgaa ataagaaaag aagttggaga
    18661 taaagtattc tttgagactt taaatgagta ctataataag tataagcata aaaatgctaa
    18721 tggaagagct tttgtggaac tatggaatag taaaggtgta gatataaata agattataag
    18781 tgaatgtaag taaattttgt gcagagtatt aaattttatt taatactctg tttttttggt
    18841 ataatataat ttataaggca gaaaaagagg tgaaggaatg ctaaagtatt gttcagtagg
    18901 gagtggtagt agtgggaatt gccactatat agggtataaa aatactaata tactagtaga
    18961 tgcaggatta agtggcaaga gaataactac aggtcttaaa gacattgatg tagatgcaga
    19021 taaattaaaa ggaatattta taacacatga gcatgttgac catataaaag gagcaggcat
    19081 attatcaaga aagtttgata taccagtatt tgctaatatt aagacctggt gttctatgaa
    19141 ggacaaacta ggtgacatta aagataaaaa tatgaaagtg tttgaaaatg ataaaaccta
    19201 ttcattaggt gatatcatag tgagaccttt ttcaatagaa catgatgcat ctgatccagt
    19261 tggatataat ttttatacag agagtgatga aaaaatgtct atagctactg atataggatg
    19321 tataactcaa aatataaaaa aacatctata taaatctaaa ttagtagtat tggaatcaaa
    19381 ttatgaccct aatatgctta tgatgggttc atatacatat gctttaaaga aaagagtaat
    19441 gtcaagcact ggtcatttat ccaatgaaga tgcagctaat ttctgcgttg aacttattaa
    19501 tgaaggaaca gaatctatat tattagcgca tttaagtaaa gaaaataatt ttcctgagtt
    19561 agcatatgag acttcaaaag gagtattagc atcaaatgat atagtagttg gacaagatgt
    19621 aaaactggat gtactatcac gaaatgatgt ttctaatgtt tatgagatga agtagaggtg
    19681 aataactttg ctaaagagaa attttgaaaa tgttgaggat aataagattt tatactctat
    19741 aaagcagggt atgattttag caatacctgc tattatgact ggttcaactg ctcttgtaat
    19801 tttgaatctt ccaataaagg cgtatcaaga atatttatca agcttgttta atggagaggt
    19861 tacaaatatt cttaacttta taaatgattc tactttagga attatttcat tgataatatt
    19921 attaacagta agttatagtt atgggaaaat acatgggagc aagtatacag tacttgttcc
    19981 tatagttgca atgtgctctt ttttagtatt ttcacatagt aatgagctta attcttatat
    20041 agaaatattt aaaacaaagt ggttatttac agctataatc gtttctatga catcttctgt
    20101 attgtttgta aaacttacag agtcttttgt tacaagtgta aagtttcata cagaaggggc
    20161 tgatgcagat tttaatatgg tagtttcagc tatagttcct ttcataatag tagtattttt
    20221 gttttctatt tttagagtga taatgataag tctaattggc agttcaaact ttcaagatat
    20281 attttataat ttattttcaa atgtgtttaa tagaatggga acaaacctta tgagtgctct
    20341 gttgtttatt tttttaatgc actttatgtg gttttttggg attcatggta gtaacgtatt
    20401 agataccgtt gcaaaaaatt tatttgaaaa tagtatggca attaacataa atttagttaa
    20461 taataatcaa ttaccaacag aaatttttac taagaccttt tttgatacaa tggttttatt
    20521 gggaggatgt ggctctttac tatgcctagt aattgcaatt tttcttagtg aaaagcgtac
    20581 aaatgtaaga aaactagcta aaattgcctc aataccagct ctttttaata taaatgagat
    20641 gctagttttt ggaataccaa ttgtttttaa ttatattatg tttgtacctt ttgtaattac
    20701 tcctataatt ttgaccatta caagttatgt ggctatgtct acaggtattg taccatgtac
    20761 agtttcagct gtagaatgga cctcacctat atttttaagt ggatatatgt ctacaggttc
    20821 aataagaggt agtttgttgc aattatttaa tctatgtgtt ggtgttatga tttatattcc
    20881 atttataaag atgtcgcaaa atagatatac atatatgttt aaaaataata tagaaaattt
    20941 aacaaattta gtaaaaaggt atgagttaac aggtgaacag cctaatcttc taaaccataa
    21001 tggtaaaata ggttcaattt caaaaatgtt ggcatcagac ttgaagtttg ctatgaaaag
    21061 aggagaaata gagctttttt atcaaccaca agtcaattat aatggagatg ttgtgggagc
    21121 agaaggatta cttagatgga aacattcgat aggagggttc atttatccac cgctagttat
    21181 aattttggct aaagaagaag ggttcttaga tgagcttgga gagtatatta ttaataaagc
    21241 atgtattgat ttaaagaaga gtgaaaagtt attgagaaat cctgttaagt tctctgtaaa
    21301 tatatctcca gaccaacttg atgaccctaa gttaccagag caaataaaaa atatcattag
    21361 tagtaatgat ataaatccta atatgttagg tatagaaata acagagcaaa ttgcattatc
    21421 aggttcacaa ataattattg aacgtatcaa tgcgatacat aacttgggaa taaagttaat
    21481 tatggatgat tttgggatgg ggcacagttc tcttatatat cttcaaaata ataactttga
    21541 tatagttaaa ttggatggtt cgttagttaa agagatactt acaaataaaa gaagcagtga
    21601 aattataatg actattgtaa atctttcaaa aaatttagat tttgatatag ttgttgagta
    21661 tgttgaaaat atagaccaaa gggataagtt atatggttta ggatgtgaaa tatatcaagg
    21721 atattattat agcaaagcaa taccttttga agagtttgta gaatatgcaa atcaagaaag
    21781 agagatataa ttttataaga gcttgtaaat tctagtaatg catagatttg taataaaatt
    21841 aagtttagga gaaatatatg aatataagta tagtagtagt aggtaaaatt aaagaaaaat
    21901 atttaaaatt aggtattgat gagttcaaga aaaggctttc taagtattgt aagttagaaa
    21961 ttatagagtt ggacgatgaa aaagctccag aaaatttgag tattaaggaa atggaaatta
    22021 taaaatacaa agaaggaaag aaaattttag ggaagataaa gcataatagt tatgttatag
    22081 ccttggctat tgatgggaag ggtttatcct ctgaagaact tgctaagact atgagtgatt
    22141 tagcagttag aggtaatagt agtctctgtt ttataatagg aggttcttta gggctttctg
    22201 atgaggtttt aggaagggct gattatagat tatctttttc taggatgact tttcctcatc
    22261 aaatgatgag attgatattg ctagaacagg tttatagggc ttataggata aataatggag
    22321 aaccgtatca taaataaatg atacggtttt tgttggtttg taacttaata atattaaaat
    22381 aacaaaaaag tattgaaagt atattgcatt taaggtagaa ttaaataaaa tattataaca
    22441 ttataatgtt tatatagttg ttaataaaaa tttggaggaa agtatatgat aaaaataatg
    22501 ttggcctgtt cagcaggaat gtctacaagt ttgttagtta ctaaaatgga gagtgcagct
    22561 aaagaaaatg gtatagagtc gcaaatttgg gcaataccag aatcaactat acaaaatgaa
    22621 atagaaaaat gtgatgtctt attactagga ccacaagtta gatatgtatt acctaaggct
    22681 caagaaattg ctaagccata taatatacca gtagaggtta taaatatgat gcactatgga
    22741 acagttaatg gagaagctgt tttaaataga gcgatagaac ttaacaacac taagtaatat
    22801 aaattataat gtatttttaa tatataaact gggagaatca aagaatggat gaaaagatta
    22861 tagaaatatc ttttaatata ataggttatg caggtgaagg taaagggtta gcttttgaag
    22921 caataaaaga ggctaagaat ggaaatatag aaaaagcaaa agaattatta aaagagtcta
    22981 aggaagttat aaataaggca catagatatc aaacagaatt aatacaaaac gaagctagtg
    23041 gaaacaagac tgaaataagt gtcatactta tacatgcaca ggaccactta atgaatggta
    23101 tgaattttca acagttagct gaggaaatcg ttgatttaca tttgaaatta caagataaat
    23161 aaaaattaaa aagattaaat taagagagtt cttaaagtat acattatgtg ttgcacacta
    23221 attgttagat tattgattca atatttagtg agtaatacat taggcttatt ttgaggactc
    23281 tttttatatt tagtttaaag ttaatagata tttattatta ataatataag attatatgtt
    23341 ataatattat aacatataat ttaagaggtg aatcatgtta agaaatcaac cattatatat
    23401 acaagttgta gatatattaa agcaaaagat attaaataac gaatataaaa ttgatgaaaa
    23461 tattccaaca gaaagggagt tagagcaaga gttcggagtc agtaaaatta ctattagaaa
    23521 agctattgag atattagaat cagaagggta tgtagagaaa aaaagtggaa aagggacaaa
    23581 agtaataagt aatagtcttt ttaataaact atcaaaagca gagtcttttt caagtatact
    23641 tttatctaaa ggtcataagt tatctaaaga gactatttca attgaaaaaa taataaatga
    23701 tacaaatcca caagtattta gtatatttgg agaaaaatgt agcaagatta ctagaatgta
    23761 ttacttagat agtaagccat atatattttt tactcattat ataccttttg atgaaaatct
    23821 aactgttgaa agttttaata atgaaagttc tttatatatg tacttatata ggagaaatat
    23881 aaatataact agatttgaag atgaattttt tatagaagaa tcagaactag aggtatcaaa
    23941 ggctttaaat ctagctaaag acatattatt aggaagaaaa agaaagacct atgatgagaa
    24001 tgataatata gttgagttat catttgcaag atataataca gaagtacaaa actatgtaat
    24061 taaatatgaa atataataag aatcctgagt ttattaaaat gataatatgc tcaggatttt
    24121 tgtttatttg taaaaataaa aaatattata acattataat gtttacaaca aaacattata
    24181 atgttataat atttttaata aataagtgat attatcaatt acataaaaat tagtttgtgt
    24241 tatgaaatcg ttattgcatt ataaataaag gataatatca ctaatacagg gggataacta
    24301 tggaaaaatt cgcaagtttt ttagagcgca agattatgcc tgtagcagcg aagataagta
    24361 atcagagaca tatgagagct gtaagagcag gtataatagc aacattacca ctaacaatag
    24421 tagggtcatt tttcacaata ttattaaaca tacctattga tgcttatatg gattttatag
    24481 ctccatttaa agcaacattg gatataccat ttagatatac tgtaggattt ttatctctat
    24541 atgctacttt tggaatagca tcatcacttg ctaaaagcta cgatttagat acaacaggtg
    24601 ttggtatgct tgcagttatg gcgtttttag tttctacaat agttccaact caagtagttg
    24661 aaccagtagg tggagttata gaagctggta gatggatgga tatggcaaaa cttaattctc
    24721 catcactatt tggagctatc gtaacatcat taattacagt tgaaatatat cgttttatga
    24781 aggaaaagaa tattacaatt aagatgccag caggagttcc agtagaggtt tcaaattcat
    24841 ttactgcatt attaccgaca gcagtaatct taatgttatt ctgggtaata agacatattt
    24901 taggatttga tataagtgct gcactaagtg ctatgttatc acctttaaaa ggattcttat
    24961 caggaaatag tttaggtggt gggctactga cagtattcct aataacttta ttctgggtac
    25021 ttggaataca tggtccagct ataatgggac cagtaataag acctatatgg gacatgtcta
    25081 tagcagaaaa tataactgca ttcacagaag gagtatcagc tcatgcaatg ccaaacatat
    25141 ttacagaaca atttttacag tggtatgtat ggataggggg agcaggggct actcttgcat
    25201 tagttgtaat gtttatgttt tctaagtcac aatatctaaa aagtttaggt agattaggat
    25261 ttttaccagg gttattcaac ataaatgaac ctataatatt tggagcacca atagttatga
    25321 atccaatact agggatacca tttataattg gacctataat aatgacaatt ctatcatatg
    25381 ttcttactat tacaggggtt atacctatga tggtagcaag attaccattt gcaatgccag
    25441 caccaatagc agcagtaatg agtactaact ggagtatact agcaggtatc ttagtattaa
    25501 ttaactttgt gatatcattt gctgtttatt atccattctt caaagttttt gaaaaacaac
    25561 aattacaaag agaacaagaa gaaatgagtg aaaatttata aaaatacata gatatagaag
    25621 ttttgcacta tataaaatat agaaaaagct tataagttat taagggtgaa ggtaggagga
    25681 atgacctttg cctttaattt tatatatttt aagtaatgta atttgaggag gagaaagatg
    25741 gaaagaagat taggaatatc agtatatcca gagcatagta acatggaaga tgataaggca
    25801 tatataaaaa tggcttcaaa atacggattt aaaagaatat ttatgtgcat gctttcaatt
    25861 acaagaccta aggaagaagt taaaaaacat tttaaagaga ttataaccta tgcaagagaa
    25921 ttaggatttg aagtaatcct tgacatagct ccaaacatat ttgatacatt acaaatatct
    25981 tatgatgatt taacattctt tagtgagtta aatgctagtg gaataaggct tgactatgga
    26041 tatgatggaa gtaaagaagc tatgatttct ttcaatccat atgatttaaa agtggaacta
    26101 aatatgagta atgatgtagc atacttggat aatataatta cttacaagcc aaatgtaaat
    26161 aatatatatg gttgtcacaa tttttatcct caaaggggta caggattgcc atatgatttt
    26221 tttgtaagat gttcaaatag atttaaaaag tatggaataa gaactgctgc ttttgttaat
    26281 tcaaaatacg caacaatcgg accttggaat ataaatgatg ggctgtgtac gcttgaaatg
    26341 catagaaact tagatattgc tacatcaact aagcacttat tcgcaacagg attgattgac
    26401 gatgtaattg taggaaattc atatgcaagt gaagatgaat taaaaagaat gtctgaagta
    26461 aataaatatc aagttgtatt taatgtagta gctgaggaaa aaacttctga agtagaaaag
    26521 ataataatgg taaatgaaca acactttaga agaggagata taactaatca ggtagcaagg
    26581 tctacagaag taagaaaaaa atattctaaa gaaaatttcc ctaaacatga taataagaac
    26641 atctttaaaa gaggagatgt agtgattggt aatgatgact ttggaaagta taaaggggaa
    26701 ttacagttga tacttcaaga ttgtacagat gaaagaaaaa ataaggtagg tagaataaag
    26761 gaagacgaaa taatactttt agactttatt gaaccttgga gtaaatttaa gattatttta
    26821 gagggagagt aaagtgaaga tagacatatt aataaaatgt ggaaaaacag tagataaaag
    26881 tataattgat atagctatat tagacgataa aataatagag gtaaaaaatc agatagatgc
    26941 ttctaagtat caggcaaaac aaattataaa cttagatgga gagaagttta ttagtgcagg
    27001 gtggatagat atacatacgc actgctacga aagactagac ctttataagg attatccaga
    27061 tgaggttgga ataaaaagtg gagttactac tgtagtagat gcagggacaa caggagcttt
    27121 agatataggt aaattttatg aagatactaa aaaatataag actaatgtat atgccttaat
    27181 aaatattgct aagcagggaa taacatctca agatgaactt tcaaatatga tgaatattga
    27241 tgaaaatgaa cttaaaaatg cagttagaaa atacaaagat tttgttgttg gaatcaaagc
    27301 tagaatgagt aaaagtgttg taatttcaaa tgatgttgaa cctttaaaag tagctaaaag
    27361 aataaaaaat gaacttaatc tacctatgat ggttcacttt gggagttctc ctccaacgat
    27421 agaggatata tttgactata tggaaaaagg agatatatta actcatatat ataatggaaa
    27481 gccaaatgga atacttagag gcaatgacgt taaaaaggaa atcatagaag ctagagaaag
    27541 aggtataatt cttgatgtag gtcatggaac cgaaagtttt agtatggacg ttgcaatgaa
    27601 atctaaggac gcaggaattt ttccagacac tataagtaca gatatataca ttaaaaatag
    27661 aataaatggt ccagtgtata atctgtctac aacaatggaa aagtttatat atatgggtta
    27721 ttcacttgaa gatataatag ataaagttac taaaaatgca gcagatgcaa taagtttaaa
    27781 aaataaaggt ttaataaaag aaggatatga tgctgattta actatatttg atatggttaa
    27841 tgaagaaaaa gaacttcaag attctttgaa taagtcagta atcacaagta cgtctataaa
    27901 accaagagca gtagtagtca atggagaata tttaaatata gacaagtctg tagaagagaa
    27961 tgagtagaag ggagaaacta ttgtatgagt agtatatttg aaaaatactc tgtaagacgt
    28021 gttataaatg cttcaggtaa aatgactata ctaggagtat caacagtaga taaggaaatt
    28081 attgaagcaa taggtgaagg gtgtaaaaat ttctttataa tagaagaact tgtaaataaa
    28141 acaggtgaat atatagcaaa tttattaggg tgtgaaagtg cactaatagt ttctagtgca
    28201 tctgctggga ttgcacagag tgtgggagca atgatagcaa aaaatgatat gagtctagtt
    28261 tacaatgtaa ataatcctaa gaaacaagtt aaaagggaaa taataattcc aaagggacat
    28321 aatgttgatt atggagttcc agtagaagta atgataagac ttggaggagg agaagttgtt
    28381 gaggccggtt atgctaatat gtgtaacaag gaacatataa taagagagat aaatgaaaat
    28441 acagcagcaa ttatgtatat taaatctcat cattgtgttc aaaagagtat gctaagtgta
    28501 ttagatgcag tacaagttgc taaagaatat aatttacctt tgatagtaga tgcagcagca
    28561 gaagaagatt taaatgtata ttatgaattg ggagcagacc taataatata cagtggtgct
    28621 aaggctatag agggccctag ctcagggtta gtaataggta agaaaaaata catagataat
    28681 gtaaagcttc aatcaaaggg tataggaaga gctatgaaaa taggaaaaga aaatattgta
    28741 ggtctaacat gtgcaattga gagatatata agtaatgata aagtaacttt aaaagaaatg
    28801 gaggacaaac taaatccatt tatagataag ataaatactc taaagggagt gagtgctagt
    28861 ataactagag attcagcagg tagagaaata cttcgtggag aaattgactt taatgaagat
    28921 ataataaata agtcaacaca acaaattata aatgaactta gaagtggaga gatagccata
    28981 tacactaggg attataaggc taatgaagga aaggttgaga ttgatataag aagtgtaatt
    29041 ggaagtgatt tagatataat ctataataga ataaaaaata tagtagaaag ttagagtgta
    29101 gagtaagtga cattataatg atttaataaa ttgaatttta ccaatggagg agataaatat
    29161 gaattgtata ccacaatatt ataaaaatag agtttgttta aatgttcttg caggaagcat
    29221 aagaaatgct aaagaagtat ataatgcttg tgaaggacat gtactagtag gagtattatc
    29281 gaaaaactat agtactgtag aatcagctat tgaagatatg aaaaaatact caaaggaaat
    29341 agataatgca atatctgtgg ggcttggagc tggagaccca aatcagtcaa atatggtatc
    29401 tcagatttct aaagagcttc agccacaaca tataaatcaa gtgtttacag gtgtggctac
    29461 atctagagca ttgcttggtc aaaatgatag tataataaat ggattagttt ctccaactgg
    29521 taaaccagga tttgtaaaaa tatccacagg tccattaagt tcaaaagaaa aagatggtat
    29581 agttcctata gaaacagcaa tagccatgat gaaagatatg gggggaagct caattaagtt
    29641 ttttccaatg ggagggctta aaacaaagga tgaatatgta aaagttgcaa aagcttgtgc
    29701 aaagcataat ttttatctag aaccaacagg aggtatagac ttagataact ttaaagagat
    29761 agttcaaatt gcacttgatg ctggtgtaga aaaagttata cctcatgttt atacatcaat
    29821 aattgataaa gaaacaggtg atacaaaagt ggaagatata gaagtacttt ataaaataat
    29881 gaaagaatta ttgggataaa tttatgaata tattagcttt tggtgaaata atgatgagac
    29941 tttcagtacc tgactacaag ttcttaacac aaaccaatga actaaattat gtaattacag
    30001 gtacgggatt aaatatatta tctgggctta aaaactttgg atacaatacc tacatgttga
    30061 caaagttgcc aaataataat gtaggtaaag catcaagtgc aaatataaga aagcttggag
    30121 taaaagatga ctttatcaca tatggaggga atcatatagg ggtgtacttt cttgaaagtg
    30181 ggtatggaga aagaccctca gaagtgactt atttaaatag gttaaatagt tcattctgtg
    30241 agagtaaatt acaagattat gatattaata aatgtctaga aggaatggat gttgttcata
    30301 tttgtggtat tgctatgcaa cttacagatt cagttaaaga gattgcttta gagctagcta
    30361 gaaagtctta tgaaaagggg ataaaagtat tctttgattt taattttaga caatcactaa
    30421 acccagaaaa gacttatgat gatttagtaa atgattataa gaaaatgcta ccatattgcc
    30481 acggattatt cgctagttat agagatataa ataagatact taatatagat aatctagata
    30541 taaaatcgag ttcagaattg attaaaaaag agtatgatat agacattgta gctggaacta
    30601 tgagaagatt tgatgatatg gacaatagat ttataaaggg atatatatca aatgatgatg
    30661 gttattttga aagtaaggag tataagttag aaatatatga cagggttggt gccggagatg
    30721 gatatgtagc aggaattata tattcatatt tggaaaatat gagtaatgaa gatatagttg
    30781 agtttggggt tgctagttca gtgttagctc atacaactta tggggataat tctttagtaa
    30841 gcacaagtca agtacttaga ctcatgaaca atgaaaaaat agatatattg agataacacc
    30901 tttatgtaga ataatatctt tattttttca gagtcacctt tgacttgatt atatttgtta
    30961 aacagagttc tttttagtga ttatgaatat taaattaaaa gttaaataat aaaatagtag
    31021 aaaatagcat taaccataga aaaaatggtt aatgctattt ttatagttaa tattgattaa
    31081 ttttatatta tagaaatgaa tttttctaat gctggatttg tatttttttt attccataca
    31141 actacaactt caattctatt ttctacttct tttataggta ctattgcaat atcacgttct
    31201 atcattgtat ctgtaatgca aaatgaaggt aaaatggata tccccatgtt agaagctact
    31261 aatgattcaa tagtattaag attatttgaa cgttgtacca cattaggcac aaaccctgcc
    31321 tcacgatatc tattttgtat catagatttt tgtccatact catctcctgt ttcctttaca
    31381 aaaataaatt tatcatgttg taattcaatt aaatcgatag aatcctcatt gtaaagtgga
    31441 tgaccaggag gcaaaagagc taccaaagaa tatctttcta cagttttata ttcaaaatct
    31501 tttaatggtt gattttctgg attaataaca aaagcaaggt ccatagaatt attttttact
    31561 aagtttaaaa gttcggttat ctcattagta caaatatcca tagatatatt agggtatgta
    31621 ttagaaaatt cttttaaata ttgttgaagt ttgttttttt cgtatcctat taaggttcca
    31681 atgctcaaag ttccaaaaaa accatgtgca aattgttgtg tttttgcaat agcatcattg
    31741 atatttttta ttatagtacg tgcctcagat aaaaaaactt ttccagctgg tgttagttca
    31801 acatgacgtt tacttcttgt aaatagagtt acatttattt gttcttctaa agcttttatt
    31861 tgctgtgtaa cagcagtttg ggaaatataa aatttttctg ctgttttggt aaaacttaga
    31921 ttttctgcta cagatataaa atattctaat tgtttagtat tcaaataatc acccctattt
    31981 aaacttgatt gtttctaaac cttatttata tataattaaa acttattata gtataagaaa
    32041 atttactttt aaagaatatt agaaaatatt tgtataaaaa tatatgtttt aagtatatct
    32101 ttacactaga ttttataaat ttattaataa gaataactta tatattccca tgaaaacgat
    32161 attggattta agtaagtttg tctcataaaa tgaaagtata tcaattgata atacttcaaa
    32221 taagaaggga gaaaaaatgg aagataaatt ttatgcaaaa ggcaaaggaa ataatggata
    32281 tattaaaaat cttgaagttt gttcctttaa taacttagac ggaacttgtg gaatgtttca
    32341 aatggctctg tacaaaagag atgaaaaata ctatttatat ggatgctgtt ttggaggaaa
    32401 taaaaaaaat ggagtaatga ttagtgatat tacagaccct tataatccac aatttataaa
    32461 acattttcaa atgttagacc ctaaagagta tcctacaaca acaactccaa aaattcaaat
    32521 agcagatgat ttaatgatag tagcaatgag ttgtggaagt ggaccaggag cacttgttga
    32581 ccaagctaaa ttagcaaata ttaagtgtga agcaggaatt agaatatata gtttaaaaga
    32641 agacccttta aatcctaagt ttttaggata ttgggattgt ggcttaaagc atgtaatggg
    32701 tgttcataga ttcatgtaca atggtggaag atatgtacat ttatcaagtg attgtgttgg
    32761 ctttgaaggt ctgatttaca gagtcataga tataataaat cctactaatc cagtggaaat
    32821 aggtaaatgg tggagaccag accaatatgc agatggatac ccaaatagaa tttttgatgc
    32881 aggagcacct cattgcccag aatttatgga taaaggatgg cttcatggac ctccatttgt
    32941 aagagacgga aaagcatatt gtggttatgg aggagctggt ttagttgtgt tagatgttga
    33001 agatttaaca agaccaagat gcttaggtga attgccattt atgcctgcat tttctagtag
    33061 acttgcaggt gcaagaactc atacagcatt accattgcca ggaagagatt tagtcgttgt
    33121 tcaaaatgaa ggagaaagat tccagttctt taaaccagat aacattacag atgttcaagc
    33181 tatgaataat atacatatgg ttgatgttag tgacccaaca aaaccaacat taattgctca
    33241 attcccatat cctgaagtgc caaaagattt cccttatcct aactttaatg ttgcaggatt
    33301 aggaaaacca gggccatttg gtccacataa tcttcatgaa ccaatggata ataagccatg
    33361 gttagagcaa agaggagata gagtatattg ctgttatttc catgcagggt taagggttta
    33421 tgatgtatca gacccatatt atatcaaaga gctagcatat tttataccac caaatccaaa
    33481 taaaacacca gaagaatctt atttcccagg attcccagga ccacgcttgg cagtaacaga
    33541 agatcttatt gttgatgata gaggctacat catcatagat gctttagatg atggattcta
    33601 tatattaaaa ataaaagaag attaaataaa tactaaagag ggagagttaa aaatgaaaat
    33661 actaggtatt tcttttggaa caaaaaatgg aaataatgac acaatgtgta aaattgcact
    33721 taaaggtgcc aaagaagcag gggctgaagt agaatttatt caaatgtcat ctattaacat
    33781 taaacattgt actggatgtt gtgcgtgcgt taagacttta ttatctggaa aaggtagtat
    33841 atgtgtgtta aaagatgatt ttgaatggtt gcttgacaaa atgaaagatg cagatggtat
    33901 agtagtttca gaccctattt ttgaagaagg ggcatcagga ttattccata ctataatgga
    33961 ccgttttgga cccagagcag atagaggaaa taacattata ggaacaaaag ttgcagaagc
    34021 tataggagga aaaattccag attcaagaat gcttaatgat aaagtaatat catttatggg
    34081 aataggaggt tctgactggg gtacaagagt tcaatgtgaa catgctatgc tagctctcat
    34141 accaatgtgg aaagttatag ataatgcttg gttcccatgg gcaaaggaat tagtaatgga
    34201 tgatacaagg cttgttcaag ttcatcaaat tggtttaaat atagttgaag cagcaaaaga
    34261 ttttgaaaaa gcttcatatc aaggagaaga aggtgtatgt ccacattgcc acaatagatt
    34321 attctacctt gagccaggta ctaaaaaggc aatttgttct ctatgtggta tagttggaga
    34381 gttagatact ataagtggaa aaactatatt tagttttcca gaagagcaat taggtcatgc
    34441 tcatgatact ttaccaggta agtttataca tggtgatgat attaagaaaa tggaaggtca
    34501 ttatgcagaa gttcgtaaaa cacaagaatt taaagaaaga aaaacatctt ataattttat
    34561 agagccatta actaaagaaa agcaagctgg aatttcatta ggataataat taaaatacta
    34621 agactctttt ttaagggatg ttagatgtta taggaggctt attgtatgaa tgaaaagcca
    34681 agttttaaac aaattgaagt tattttagca gttatttttt tggtacttac atattacaag
    34741 gttagattaa ctccagcatt tattgttata tttataccac tatatttaag ttattttgca
    34801 gtatctatga ttgtaaatca aatagcaatt aagctattgg agataatgca agttagggaa
    34861 gctactaatt acaatatagg aaatggatag aaagaaaaaa gaaatattaa cactgtctat
    34921 ggggattgct ttactacctc ctttatgggc agtattagca ctttatatgg gaataaaaac
    34981 tggagctgta gcactaatct gtgcaggatt atatgtaact aatggtaata aacaaaaaga
    35041 tggattaaaa ataatgtttg gtttttggtg tggagattta tgggcagtgt tagccatttt
    35101 aattatggga tatatgaatt tcaatcaaaa cctagaatta tttttaacac ttagtatatt
    35161 aggttttttt gcagttgtaa ttgcatcctt atttgaaaaa ataattttcc taccatcatg
    35221 gctttgtggt tgggctatag gtctaactat tatgacaggt gaaaatatta ttaatttaca
    35281 agatatttgt attcaaattg gtgtagctat gtcagtagga gtatggtatg tgggtgcagg
    35341 tgtagatttg tttacgagat acattttgag taaagataag aaaaagagaa attgaaagca
    35401 tgtgaatata ctttgattat aggagatatg gttatgggtt ttgaatttaa aataatgaga
    35461 agtttaatat atgtaggact tgctaaagaa gagtatagac ctaagttaat ggactggtta
    35521 tatcgtcatc atattccaga tagtattagc acttttggac catattgtac taaatatgcc
    35581 ttttatcaag catatcctac accaaatgaa ggtgagcgtt ttggtgtacg taagatgcaa
    35641 ctaacagaac attattggct tgtagatgaa catatgcctg agatggcaaa tagaattatg
    35701 acagaataca tgcccatgga tgttctacgt tggcaagggt gtataccaga tgtagaaaat
    35761 aaaagggttc atgaaaatgc agaaagtgga gatgcaggac gtgcagtagg tggagataat
    35821 ggatgtccac catttatatt tgcctttgtt ccaataaatt gggaagaaga ttttagagga
    35881 aaaggacgta ctgtacaaga tggaccaaac tatcgttggc aatttatgat taagtatcca
    35941 gatggtattt ctaaagaaga aggagaaaaa tggttctatg atgaggtagt gccatacttt
    36001 acaaactgtt gctatgttaa tcgttttgtc agtagtaaaa taatgattaa ttatggagca
    36061 actgcttttg accgtgtatc agaactatgg tttgaagggg aagaagaatg gtataaagct
    36121 gtggttgaag agacaaagtc gtttattaaa aaaccagaat gggcacaaga agaggagttc
    36181 ccatatttaa aaccacaatt caatatcgca tcagtattct taggtgatat agcaactatg
    36241 gatgcatact cacagtatcg tggatatata ccaatgagat aaaaattata gtttgtgata
    36301 agtagaggag gcatttactc ttactggtag aagaagactt attgtatcct taaaagtagg
    36361 tgaatatata gttttatatt taaatttata taaaactaag actaataaat gtgaaacaat
    36421 agaattagaa taatttgata atatttgtta aaagagtatt tagaatgtgt ttaaagatta
    36481 agatgttcct tttatggtgg cagatagttg aaataataaa actgttatca tgaaaggaat
    36541 atttttatgt atatgaaagc ttttaaaata aaagttaaat atgtttaaga acatcttaaa
    36601 gtgaaagaac cattattaga aatatgtaga caatatagta tttataacgt atcatattaa
    36661 caagtttaca cttgggttat gaaacaaaaa gagtaattca aaagattgta aatactatgc
    36721 tgactttgat acttcaaagt ttagatgata ctgtaattgt taatagaaat acgtttaatg
    36781 agcataaaga atttatttaa taataggaga atataatgta tacaattgga caagtatcta
    36841 aatttttagg tatatctaga gacacactaa aattttatga ggacaaagga ttagtaaatc
    36901 ctaagaaaaa tgatgaaaat ggatatagaa tatataacaa ttttgatatt tatgatattg
    36961 caacaataaa cttttataga gaaattgata tagaaattaa aaagattcaa gaaataagaa
    37021 aaagtaagag tataaacaat ttagaactct tactagaaga gaaggaacaa atcatactaa
    37081 aagagataga atataaaaag cttctcttaa aaaaggttca gttagtcaaa gaagattgtg
    37141 acaaaatcaa aacatatttt ggaaaataca caataaaaga aatgaagcct ttggaagtaa
    37201 agggtagaat aaaacatgtt actgactatg atgaatatgg ggccttaaaa gagaatacaa
    37261 acaacttaaa aaaagcagtt acattaactg atttaagaag agtaataagt tttaatgaag
    37321 aaggtataat agaagaaaaa tttataattg taaaggaagt agatgactat aacaaaatca
    37381 tagaaggtga agttctgtct catcccaatt gtatttatac tattattgaa gatggcagat
    37441 ggtcaactgg aggagaaaat attgataata aagtggaaga cagattaaga gaaatagcaa
    37501 taaaaaatga atatgaatta cttggaatag tctatgttaa tatattgctt acgacttatg
    37561 aagaagagat tgagcgtata tttttagaga tttatgcacc tataaaataa tataaaagca
    37621 ttgacctggg acttactcat aggtttaaac tcatgatata agcagttata acttatatag
    37681 cagttaaata ttaatttgaa atgaggatta cttatgggaa cagagaaatc attaaaaagg
    37741 cctgtagcca gttttattat tctaacaaat ataatttttt taccactttt tttattggta
    37801 gtagtgacta taattttaaa acttccaaca gtgatatctg acattgcgtt gtgtatatca
    37861 gcgtggtcat ccacttttgc atttatgata ttatttaaaa aaatttatcc aggtaagaaa
    37921 tttttagtac acgtaaaaga tagatttaga aataaactta aattttcaac agtatctata
    37981 ataattagta tacaagtcct aatagctgta gtagttatat ttatattagc atctaaaaat
    38041 catggaataa tgcctaactt tacagtttca tcatgggggg catttatata tttattttta
    38101 aaaaatttat ttgcaggatc attaggtgag gaattaggtt ggagaggatt tgttcaaaat
    38161 catcttcaga aaagatattc tcctttaaaa gcatccataa tagtaggttt ttggtggggg
    38221 atgtggcatt taccaatatg gtttacaaca ggattcaaag ggcttgattt aataaaatat
    38281 attatatttt ttatgattgc aattatatca atcagtatta taatgacaac tttttataat
    38341 ctaaataaaa ctttgataat acctattgca atacatcaac tatttaattt tttaataggg
    38401 ataattaatg gagatttaat agagcttata aattactatg caatgttata tttaatattt
    38461 gcaattgcat taatagttat aaatccagga aatgtattat ataaaaatat tagagagtaa
    38521 tatatatctt tatggtaaga tataaagaaa ttattagaag aacatccaag gaggaataaa
    38581 catggtttta ctgattgttg atgcacaaaa gttaataaca aacgagaggt tatataaatt
    38641 taatgaattt gtagtaaatg tagaaaattt aattgataca gctagaaaaa ataatataga
    38701 agtaatatat gtacgtcatg atgatggagt tgaaaatgaa ttgactaaag gtaaaaatgg
    38761 ctttgaaata tacgaaaaat ttaaaccatg taatggagaa aaaatatttg ataaaaaagt
    38821 aaatagtgct tttaaagaaa ctggtctatt agaataccta acaaataatg gtgaaaaaga
    38881 tattattatt gctggtctcc aaacagatta ttgtattgat gccactataa aatgtggatt
    38941 tgaacatgga tttaatataa tagttccagc ttattcaaat acaactgttg acaatttttt
    39001 tatgtcagca gaacaatcat ataaatacta caatgaattt atatggaatg aaagatatgc
    39061 gaagtgtatt tcattagagg aaacacttag aaaaatgaaa taaaaataga gtatgaattg
    39121 tatatgtaca taagttgatt tcataaaaaa ggatgttaaa ttccaagtta aaaattagat
    39181 taaatatatt atattataga taaggcacat caattttatt ttaaattaat gtgcttttta
    39241 attgtagtaa tttatttata atcaatatga tttttattat ttaaatcaat ttttcttgag
    39301 tttctttgtc taaagaaact ttttttattt ataaaatcta cagttatatt atagtatcta
    39361 ttttagagaa gtttaatttt tgtatcaatc taaaacaaaa gttatactta aatgaaattg
    39421 aaaacgataa aattctcaaa taaaataagt attgtggagg tgatgaaaga atccaatgat
    39481 aatatgaaga ctaatcttaa taagaaagta ggggttaatg gtagtggaca aagaaattgt
    39541 atcaattctc cagattttat taaatagcac attgattaca actaatggat tacaagagga
    39601 gtctaattca tcaaagagac aaattactta tagaataaat aaaatcaatg acatgcttaa
    39661 agtaaaaaat gtaccactta tatgtttaag agctgataaa gatattatcg ttagaaaaga
    39721 aacaaaaaaa gcaattaaag aaattttaga aaaaaactat tctaagaata cttattattt
    39781 tagtaagaca gaaagacttt tatatatgta tttaatgctt tttattaaca tggaggatat
    39841 ttcaattaat cattttataa attctataaa agtaagtagg tctactgtaa atcttgattt
    39901 caaggatttg ataccagagc ttgaaaagaa gaacattaaa gttaagaaca atcgtataaa
    39961 tggatattac atagttggca atgagatgga aattaggaga gttcttatta aaaacattat
    40021 agaaactttg tctaatgaga aaagttcaag agtatttgat atttttatta aagaatataa
    40081 tttagatagt tttgaagagg ctaaaaaaat tatattaaag ctaataaaaa aatatgaaat
    40141 tacttttgtt gaagatagat tagtagaatt tatatatata tttatctttc taaaggcaag
    40201 gatgtattca actagaatag ttactcagga aaatgtagat attcctaata tttcagttat
    40261 gagttcaatg aaggagtata aatttgctaa ggaattatta gaagtatatg ggagtgaaga
    40321 taagataaag cctcataata taatgtatat tagtgcttgg attttaggta tatcagtagg
    40381 aaattatgaa gaagatacag aagataagga tgtaatttca aaaattgtaa ataaaatgat
    40441 gacaaagttt ggatatttaa gcggagttta ctatataagc caagaaaaaa tatttaaaca
    40501 attatattct cattttagac ctgcatacta tagactttta tttaaattac ctatttataa
    40561 tccgatttgt gaaaaagtaa aagaagaata taaattagtt tatagaattg taagtaatgc
    40621 aatgaaagag ttttgcggat tatttggaga agaaattcag gaagaagagt tagcatatct
    40681 tacaatgcac tttgcaacac ttttttcaaa taaaaaagaa tttgatggtt ttagaaaaag
    40741 aatagcatta atagtttgtt caaatggcgt tggtagttca gctattttac atgcagaact
    40801 tacaaactta tttcctgact tacactttat accaatgtcg gaatcagttg gattagaaaa
    40861 tataagtgaa cctgtagata ttgtatttac tactaattat aacgcagagg gtttaaaaac
    40921 agatgtacca gtaattaaag taagccctgt tatgaccaca aaagaaaaat ataagattac
    40981 acgtgaagtg tatattcaat taggtgatat ttatttaaga caacctaaaa tagatgaggt
    41041 tatggatata ataaaaaaat atgcaaatgt aacctctgaa agtattcttt caagtgagct
    41101 attagcttat tttacacaga tagagaattt tacatctaag gaaggagagg ggcctatgct
    41161 aagtgagatt actaatgaaa cactagtaaa aataaaggta aaagctaaga attgggagga
    41221 agctattaga cagtcagctt ctgtattagt tgaaaataac aaagtaactg aagggtatgt
    41281 agatgctatg attaactcag caaaagcatc tggaccatac atagtaatta caaaacatgt
    41341 tgcacttcca catgctagac cagaagctgg gtcaaaagag attgcaatag gcattgctac
    41401 tttagatagt cctattgagt ttgggaatgc tgaaaatgac cctgtaaaat atgtattttg
    41461 tctaagtgca atagacaata atagccattt acgtgctatg tctgagctag ttgaactatt
    41521 ggaagaagat gagtttttta aggttttaga tagtgctaaa gaggctaaag aaattattga
    41581 ttatataaaa acacacgaat tataaaggag gatttaatta tgtataaaat tttaatcgca
    41641 tgtagagcag gagttggttc aagtttaatg ttaaaaataa agactcaaca aattattaaa
    41701 gaaaataact ttccaattga agtagaacat ggttcattgg attcactaaa tgggttcaat
    41761 ggagatgctg tagaaacatt aattgatgtt gctgaagaat taaaaaataa aaatttgaag
    41821 tttgatataa ttgggattca caatattgtt gaccgtaatg aaataaagac agaattagaa
    41881 aaattccttg cttcaaaaga gggataaatt taagatgata aggaggaata tttatggagt
    41941 ttattattag tttgctgagt acacctgctg ttttgcttgg tgtagttgca tgtataggat
    42001 tgattcttca aaagaaatca gccattgaag tatttacagg gtcttcaaaa acacttatag
    42061 gatttttgat atttggtatt ggtgcctctg ctatgacagg agcacttcaa aatttcaata
    42121 aattgttcca acatggcttt ggaattactg gtgttatagc ttcaccagaa gcagctacag
    42181 cacttgcaca aggtacatat ggttttgcag tttcatgtac attaattctt ggattcatat
    42241 taaatttagt atttgcacgt attacaaagt ttaagaatat tttctttacg acaggtcata
    42301 gtttgttttt ttcttgtgta ttagttttaa tcatgaaagc acatggattc gataatacaa
    42361 tcactatttt aataggtgga actatcttag ggtttatgtc tgctgcacta cctcaattct
    42421 gtcaaccatt tatgcgtgaa ttaacaggtg gcgacgaaca agctattggt cattttaaca
    42481 tgattggtta tggtctatca ggttacatag gacgtttatt ttcaaagcat aaagatgata
    42541 caacagagac tatagaattt cctaagtggt tatcaatatt tagagacttc ttaatgggtc
    42601 tatccatagt tatgttaatc ttgttttata ttgcaacatt aaaagctggt aaagtattta
    42661 ctcaagaaat tgctggttca acacactggt tggtatttcc gcttatagag gcatttacat
    42721 ttgtggcagg tatgtcaata ttgatgtcag gagttcgtat gttcttagct gaaataactg
    42781 cagcatttgt aatcatttca gaaaagtata ttccaggttc acgtcctgcg ctagatgttc
    42841 caaccgtatt tccatatgca ccaaatgcag ttattattgg gtttatatca gcatatgctg
    42901 ctggtctatt agcaattgct attatggcat catttcctag tatatttcct gtagtaatta
    42961 ttccagctgc tcacatatgt tttttctcag gaggtacagc tgcaatattt ggcaatactt
    43021 caggaggatg gagaggggct attgctgggt catttgttgt ggggttatta cttgcatttt
    43081 taccagttat actatatcca gtttatggga ctcttggtat agaaggagca actttcccta
    43141 atattgatta caacgtagtt ggttcgatat tgcataatat tttacaattt ataaaaccta
    43201 tttaaactaa attatataaa agtatagtca tagttactat gatttcaagg aggatacatc
    43261 ttatggaaaa tacattatct gaatttttag ataatatatc taatgaattg tcagaattta
    43321 ttgcctcaat agatgagaca tctataaata aagcatgcga attaattctt gaagctgaaa
    43381 aaaatcacgg acgtgtacat gtgactggta ttggcaaacc aggacatgta tcgggatata
    43441 tttcatcatt gctttcatct actggaacgt cagcatatat attgcatggt acagaagcag
    43501 tacatgggtc aagtggtcaa gtagttgaag gagatgttgt tattgcaata tcaaatagtg
    43561 gtgaaactca agagttaaaa gcaactctta agactctaaa agtaaatgga gcaaagataa
    43621 ttggtgtaag tggtaatgag agttcatttt taaaaaatat ttctgatata ttcctgtttg
    43681 caggtgttaa acaggaagga gattgtctta ataaagctcc tcgtgcttca attttagctg
    43741 aaacaataat attacaatca ttaagtgttg tattacaata tgctaaaggt cttaatacac
    43801 agcagtattt gaagtggcat ccagcaggaa gtcttggaaa gtctattaga gagggaatat
    43861 aattttatga aatataaagg gattatatgt gcaatgataa caccatttga tgaaaatcaa
    43921 aatattaatt cacaagcaac atgtcaatta attgattatt tgattgaaaa gggaatttat
    43981 ggattattta ttttgggcac taatggtgaa tgtcatgtat taacagatga tgaaaaagtt
    44041 gaatttgcaa agattgttat taatcataca aataatcgtg tacctgtttt tgttggaaca
    44101 ggtggtaatt caactcgtga agttgtagat ttatctaaga gaatggaagc gattggagca
    44161 agtgcattat ctattattac accatgcttt gttacaccaa cacaacagga attaatctta
    44221 cattacaaaa ctattgctaa ttcagttaat ttacctatta taatgtataa tatgccaggt
    44281 aagactggaa ttaatattga accagagtca gtacgtgaat tatcaaagat aaagaatatt
    44341 gttggaatta aggatagtag tggtaaactt gacaacatga aagcttatat tgaagcaacg
    44401 aagaatgaga attttagtgt gttttcaggc tcagattcac ttattctgga tagtctaaaa
    44461 gctggtagtc aaggagctgt tgcagcaaca acaaacttcc tcacagagat tggtatctct
    44521 atctatgaaa actttatgaa aggggatttg gaagaagctc aaaaagctca aagtagtata
    44581 gaagaacttc gacgtatact gaaacttgga acaattccat ctgttatgaa gaaaactatt
    44641 gtcttgaatg gaattaatgt tggaactgca cgacttcctg taactgaacc aactggggaa
    44701 gtacttgaag aaattaaacg agtagttgaa aattatagga caatacttaa taaaagtagt
    44761 gagaatagat aatataaata gggaaattac actggtatat aattaatata tatattataa
    44821 aacatgacta aaattaatga ataagtaaag tggtttgatg agtatagagg tgataatatg
    44881 aaaaagatga atataaccaa aagaatgtca gaatgtgggg ctcttgcaat tgtaagagaa
    44941 gaaaatctta accgtgcatg tgaaattgct gaaggatgta taaaaggtgg tataacagtt
    45001 atagaaatga gttataccat aaataatgct ggagaaataa ttcaagggct taataaaaag
    45061 tatggtgaaa tactttgtgt gggagcagga acagttttgg atagtgaaac agctcgtcat
    45121 gctgtattac atggagctca atttattatt gcaccaaatt acaatgaagg tgtagcgaag
    45181 atttgcaata gatatcagat tccttatgct ccaggatgta catcattgac tgaggcagta
    45241 gatgcactaa gtttaggagc aacttttata aaagctttcc caatatcaga tttctatgga
    45301 cctaaactag taaaggtatt taagacacca atacctgata tgccagtttt agcaagtgga
    45361 gggattaata ttgaaaacct acatatatgg ttagaaaatg gagttgatgt atgtggattt
    45421 ggtggtttat taactaaagg aactgttgag gatattactg aaaatgctaa aaagattcgc
    45481 gaaatcatta aaaatataag agaagtttaa catggaggat gaattagttt tctattgtcg
    45541 agatatccac acattaattc ctaagctaga agaaatgttt tctagtcaag gtaaagttta
    45601 tattgaactt aaattaaatt ttgttttaaa taagatatta ttttagttga ttacttaaaa
    45661 tactagaatc catattaatg aattacacta attttgtact actattaaaa tattgttttc
    45721 taaaaataaa taataacctt aaaacagttg attataaaaa attaactgtt ttttgttatt
    45781 tttatctttg tgacgaaata gacacttgcc atttagaatg tgacaatata gtagttgttt
    45841 agaaaagaca caaagcatta tactttaact aaagtaaaat gtattttgct tttagaataa
    45901 aaataattag ggaggagcta taattgaaaa agataatata tgtactattt acaattgaac
    45961 tattaatagt atctttatta ggtctaaata tagttaaaga taatgaaatt aataat
//
LOCUS       DAEMAE010000008        44720 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEMAE010000008 DAEMAE010000000
VERSION     DAEMAE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.008"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(150..1400)
                     /gene="cme"
                     /locus_tag="KPH93_000387"
     CDS             complement(150..1400)
                     /gene="cme"
                     /locus_tag="KPH93_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG1687224.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAIIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGIQTPAVNAMIPQLVPEEHLMRFNGINGSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVMSINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1486..2232)
                     /locus_tag="KPH93_000388"
     CDS             complement(1486..2232)
                     /locus_tag="KPH93_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1687225.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNKYLQQLEIEKSN
                     AEEALTIVTQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKFHLQIMKKQFS"
     gene            complement(2347..3369)
                     /locus_tag="KPH93_000389"
     CDS             complement(2347..3369)
                     /locus_tag="KPH93_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG1687226.1"
                     /translation="MNKNNFLDLIFKKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEVCKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKIIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSTGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYNLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3369..5789)
                     /locus_tag="KPH93_000390"
     CDS             complement(3369..5789)
                     /locus_tag="KPH93_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1687227.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRARITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLIDQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYISGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGNFSFNSKGACSKCSGSGYEKVWLG
                     NNLSIDHTCSECQGKRFNDESLSIKYKNKNIHDILNMSVSEAVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTAK
                     LIQLLDELVSNGNSVIVVEHDIDVLNVCDWIIELGPEGGDKGGYIIAEGSPKVLKENP
                     KSITGRYL"
     gene            complement(5875..6795)
                     /locus_tag="KPH93_000391"
     CDS             complement(5875..6795)
                     /locus_tag="KPH93_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1687228.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTITDIAHYFGFTDSAVFSRAFKN
                     HYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSDGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            7404..8330
                     /locus_tag="KPH93_000392"
     CDS             7404..8330
                     /locus_tag="KPH93_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG1687229.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDINRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNSCSVYSYVCSENS
                     NAEIVAKNGDIFKNIEKYIDVTINNNQKTMKYKHRDELFKNSNTNIYSRISEESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLSEESSLSACNYGDMCYEDMY"
     gene            complement(8530..9150)
                     /locus_tag="KPH93_000393"
     CDS             complement(8530..9150)
                     /locus_tag="KPH93_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687230.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHSLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDNLIDSLLKVYQGGTCFPSENTEVIIEDLTSMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIQMGYISPVY"
     gene            complement(9122..10705)
                     /locus_tag="KPH93_000394"
     CDS             complement(9122..10705)
                     /locus_tag="KPH93_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687231.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVATPIGTIIFKILSTVIAYVS
                     IKFIFLFLFQGSGYKFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHWKRVAFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRSIFLFISILVISLVVLFASGNFYIILIAINVL
                     FMLIFLCNIILEFNLRNEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(10849..12876)
                     /locus_tag="KPH93_000395"
     CDS             complement(10849..12876)
                     /locus_tag="KPH93_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687232.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNLISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTSENILICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLIKLIRYEKSTEKKLKFRKIPAFMGFIMILSGYSLS
                     LDILRGAKSVWISIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRAFYTGTK
                     IITIPNFIYRIRSNSKTLIMLTLLSAATLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYMSNTDDIIFTQTNIYKVKSSSKDLPLEYEIGTSKNNLSNRNS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLIKQLIPLRDNECILMKYQPNNDRYSEN
                     GTIYPLLINGKETPVTVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIASG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            complement(12889..13635)
                     /locus_tag="KPH93_000396"
     CDS             complement(12889..13635)
                     /locus_tag="KPH93_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687233.1"
                     /translation="MNTILKVNSITKTYKGQQPSLDKVSFNVSEGEFIGIMGASGSGK
                     TTLLNVLSTIDKPTSGDIFINNINLKSLNDTSAADFRKDNLGFIFQEYFLLDSLTIFE
                     NISVPLTLQKLSPDKIEKMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKHP
                     SILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDVYAASFANRILIFKDGH
                     IIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            complement(13932..15593)
                     /locus_tag="KPH93_000397"
     CDS             complement(13932..15593)
                     /locus_tag="KPH93_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG1687234.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKSELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAIV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLKNRSIKKSYIVGGNRILSD
                     NAVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKKILKQALPGSNILVCYYDEDDNKYDCKVIKDNVLYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDKDSDDDFDDNDNNHNNSSNTNDSNSIIGAEKAKSIMLDKVH
                     GGKIVKFEYDEDDKEYEGEIIKGKTKYEITINAITGEILEFEIDD"
     gene            16192..17313
                     /locus_tag="KPH93_000398"
     CDS             16192..17313
                     /locus_tag="KPH93_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG1687235.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTVATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAEIFEK"
     gene            17349..17762
                     /locus_tag="KPH93_000399"
     CDS             17349..17762
                     /locus_tag="KPH93_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1687236.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEEVSYE"
     gene            17755..18294
                     /locus_tag="KPH93_000400"
     CDS             17755..18294
                     /locus_tag="KPH93_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1687237.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGALESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVSAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLV"
     gene            complement(18494..18979)
                     /locus_tag="KPH93_000401"
     CDS             complement(18494..18979)
                     /locus_tag="KPH93_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687238.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKFYLTCIGSGHYFANMEEIDWKNLEKLITKENNIMQES
                     V"
     gene            19883..20260
                     /locus_tag="KPH93_000402"
     CDS             19883..20260
                     /locus_tag="KPH93_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG1687239.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            20656..22461
                     /locus_tag="KPH93_000403"
     CDS             20656..22461
                     /locus_tag="KPH93_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1687240.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     ESMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLSKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            22537..23076
                     /locus_tag="KPH93_000404"
     CDS             22537..23076
                     /locus_tag="KPH93_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG1687241.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            23681..24895
                     /gene="dpaL"
                     /locus_tag="KPH93_000405"
     CDS             23681..24895
                     /gene="dpaL"
                     /locus_tag="KPH93_000405"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG1687242.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILGSEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGDCQSK"
     gene            25143..26330
                     /locus_tag="KPH93_000406"
     CDS             25143..26330
                     /locus_tag="KPH93_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG1687243.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            26346..27929
                     /locus_tag="KPH93_000407"
     CDS             26346..27929
                     /locus_tag="KPH93_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG1687244.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKTNWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            27957..29285
                     /gene="ssnA"
                     /locus_tag="KPH93_000408"
     CDS             27957..29285
                     /gene="ssnA"
                     /locus_tag="KPH93_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG1687245.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISTDEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            29484..30836
                     /locus_tag="KPH93_000409"
     CDS             29484..30836
                     /locus_tag="KPH93_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG1687246.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     NAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            31032..32141
                     /locus_tag="KPH93_000410"
     CDS             31032..32141
                     /locus_tag="KPH93_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1687247.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIRGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            32420..33811
                     /gene="hydA"
                     /locus_tag="KPH93_000411"
     CDS             32420..33811
                     /gene="hydA"
                     /locus_tag="KPH93_000411"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG1687248.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGFDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKAENNLSPKYHQLTRPAILEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAISDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEVKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            33805..36366
                     /gene="xdh"
                     /locus_tag="KPH93_000412"
     CDS             33805..36366
                     /gene="xdh"
                     /locus_tag="KPH93_000412"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG1687249.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVMLDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            36518..37864
                     /gene="rpoN"
                     /locus_tag="KPH93_000413"
     CDS             36518..37864
                     /gene="rpoN"
                     /locus_tag="KPH93_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG1687250.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            38660..39682
                     /locus_tag="KPH93_000414"
     CDS             38660..39682
                     /locus_tag="KPH93_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1687251.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            39742..40749
                     /gene="gap"
                     /locus_tag="KPH93_000415"
     CDS             39742..40749
                     /gene="gap"
                     /locus_tag="KPH93_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG1687252.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            40971..42173
                     /locus_tag="KPH93_000416"
     CDS             40971..42173
                     /locus_tag="KPH93_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG1687253.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTKDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            42199..42942
                     /locus_tag="KPH93_000417"
     CDS             42199..42942
                     /locus_tag="KPH93_000417"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG1687254.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKYKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKIQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            42951..44483
                     /locus_tag="KPH93_000418"
     CDS             42951..44483
                     /locus_tag="KPH93_000418"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG1687255.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMSKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKSFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALEKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAVKAVETVDTCVGKLVDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT        16999 a         5587 c         7786 g        14348 t
ORIGIN      
        1 gctcaaaata aagataaacc tatttttcaa tgttgaaatg ataaaaacat atttgatttt
       61 atataaaaaa ttttcttcat aaattaaaaa gtccattttg agacccctca tttttaacta
      121 gataaatcga gttaaactat ttaaatatat tactcagatt catttgactt agatattcct
      181 tgcttgtaaa attgttttga gtaggggata gccaaagcaa aaaataaaag tataatccct
      241 gctacgatta ttaaattatt aatagacatt acatctgcca ttggaccgaa aatcatcata
      301 ccaagaggaa taaaaccagt atatataata ataggcaagc taagaactcg acccagcatt
      361 tgtggtgcta catgctcttg caacatagtc atagctgatg cttgaattat tggaattgaa
      421 aaattcgtaa aaaacataaa tataaaaaat tgccaagctt gtgttacaag acctagaata
      481 attgaaaaaa tagcatagga taatattgct agaaaaaagg tcttatttct atttttaaaa
      541 ccaccaaaaa ctcctataat caatccgcct aaaaccatac ctaaaaatcc aatcatttca
      601 ataatagaca ggtatatata gcccttacca aatgttcgtt caataagaag tcctataaga
      661 aatcctgatg gaacacaaag aagcatgaat gctccataag tgaaaagtac ttttctaatg
      721 aatttatctg aaaaacagta ctttatccct gataccatat ctgcaaaaaa tgagactttt
      781 tcagtttgtt ttggtatatc atgtttagga attttgatga atatcaataa tacaatagca
      841 ataattgcag taaatacatc aattaaaaga acttgattta ttttattacc aaatccaagt
      901 attgcagcag ccacaatagg aactataaat tgtactatag actgtaggct accatttata
      961 ccattaaaac gcattaaatg ttcttctggt acaagttgag gaatcattgc gtttacagca
     1021 ggtgtttgta taccacttcc tagtgaacgt ataacagata ttactgatat ggctattaat
     1081 tcacttgttg tgccatttga tgacatcata aatagaatta gaactaatgt agatatagca
     1141 attgttgaat ctgatacaat tatgagtacc tttcgattgt agtagtctgc ccatacacca
     1201 gcaaaaaaag aaattatcat ttgaggtata aaggagcata cagttaaaac tgttacccat
     1261 acacctgatg atgtctgaag tgtgacatac catattattg ccatttgtac aagtgatgag
     1321 ccaaataagg aaatatattg acttgctaaa aaaagaatga cttttttctt ccatgtagaa
     1381 ttagtatttt tgttttccat cctattcctc ctttgatttg tgtgttgaat ttttcttgct
     1441 cttaaggata acacacaaat caaagtctgg agttagagtg gagggctagg aaaattgttt
     1501 tttcataatt tgaagatgaa atttcatggt ttttgcatta tgttcagctt ctttcaatga
     1561 agttatcagt ttgtcacaca ctgagataat ttcgtcatca tcttttggag tgtcaaggac
     1621 agttttaata ttagtattag gattagaaga taaagcattt aacattcgta aaatagctga
     1681 aagagaatag tttgcacaac gaagtgcgct tataatttta agtcgcttta tatcttcatc
     1741 agtatataca cgataaccat tttgttttct ttttactttt aacaaaccat tcatttccca
     1801 atttcttaaa gtatctattg taacatttaa gtgttctgca gtttcttttc tagttaaaaa
     1861 gagtttattt gtttctggag tatttccatt taaaatctgt gtaacaatag taagtgcttc
     1921 ctcggcatta gatttttcta tttctaattg ttgcaagtat ttatttacaa gactaatagc
     1981 cttatcaaag tccctatttg ctgatgttct tacaatctca agggcatctt ttcttaatcc
     2041 cctttgtacc acttcaattt tcaaggctgt acgagcaaat ctaaattgtt ctatatgaaa
     2101 atcattaaag atacgataac cgttggtatt acgcttaggt ttaggtatta agtttagttc
     2161 ttcatataaa cgtactgtat taggatgtac accaattaaa tctgctacct ctgatgtcgt
     2221 gtaagtgtcc attagtattc aactcctttt agatatatag tactaatcat aataacacca
     2281 aacaaaagtc tggtgttata gtggagggtt tggttatcta agagtaatta gtatgtctaa
     2341 gagtgattaa tctattatag aagtaagtat atatttttca ataaactccc atctatcacg
     2401 aggtttaaac actattttag atggtatggc tattactaag ttatattgtg gaatacaaca
     2461 aatagcattt cctccagttc ccattgccga ataccacaaa actccatctt ttttacatag
     2521 ccaccacaga taaccatatt cattactatt cattttaact gattcttcaa tccacgattt
     2581 agatataata cgtttattat tccaaatacc atcatttaaa tacagaaaac caaatcgcaa
     2641 catatctcta gcactcaatg taagtcccca gccacctgta gaatagccct gtctatcttt
     2701 tgcccaaccc ttaagatttt ttccaaagaa atcttcaaaa ccaaaatctt tcatatcata
     2761 atctggtata attttcatac caataggttt aaataagtac tcattagcaa attcacgagc
     2821 agattttcca gtagtttttg ttataatagc tgaaagtagg tgtgttccag ctgaagaata
     2881 tttgaaagta ccaatttcac ctttttgacc aagctgattc aaggtatatt ttatccaatc
     2941 tttttgtttg caaagtctct ctaaagactc agtcatattt ttaaaagggt atggtgcagt
     3001 cattgtaagt aggtgacgta tagttactgc ttttttacat acttcagaag gactacaagt
     3061 atattcaggg aaaaaatcaa caaccttttg gtcaacatct tttatatatc ctttatctat
     3121 tgcaatgcct attaaggctg atataatact ttttgtaact gatgcaacat gaaaagtatc
     3181 atcagcatta tatccattat agtacttttc aaagacaact tttccttttc tagcaataat
     3241 tattccattt atattactat attgtgagat tattaaaggt tcaagttgtg aaagcttttc
     3301 tgaactcatg ccaacacttg aagggtctac ttttttaaat attaagtcta agaaattgtt
     3361 tttattcact aaagatacct ccctgttata gattttggat tttcttttag aacttttgga
     3421 gaaccttctg caattatata acctcctttg tcaccacctt ctggaccaag ctcaataatc
     3481 cagtcacaaa catttaatac atctatatca tgttcaacaa caattacaga atttccattt
     3541 gatactagtt catctaataa ttgaatcagc ttagcagtat cgtatagact tagaccagta
     3601 gttggctcat ccagtacata aaggatatta ccatttcgtt ttttaccaat ttcttttgca
     3661 agcttgattc tctgggattc accaccactt aaagttggtg taggttgacc taattttata
     3721 taccccatac ctatttgttc taatacattt aagtgtgaaa caatatttgg aatatctttg
     3781 aagaaatgta cagcttcaga gacactcata tttaaaatat catgtatatt tttattttta
     3841 tatttaatag ataaagattc atcattaaat ctttttccct gacattcact gcatgtatgg
     3901 tctatactta gattatttcc taaccagacc ttttcatatc cactaccact acatttagaa
     3961 caagcacctt tggagttaaa tgagaaatta ccagctgtaa atcctcgttc taacgattct
     4021 ggctgacctg caaatagtgt acgtatctta tcccatatac caatataaga agcaggagtt
     4081 gagttcatat ttttgcctat aggagattga gatatctctg aatatccaga aatatgttta
     4141 gtaccaatca gtctatctgc aatagtttca acaactgcat aatcgtcttc atcacctatt
     4201 tcgctctctg aagaatttgt ttgattattg atagtatggt tattaaattg gcttctaaga
     4261 agaggaagta gggtatctga tataagtgaa ctcttgccac tacctgattt tccagatatt
     4321 ccaaccatag cacccaaagg taatgataca gttacatttt taagattatt tgtatttgca
     4381 ttttgaatag ttatacaagg tattttatca gtatgtgtaa tatttttatt gcttgttcta
     4441 ttaggcattg gatattttcc agatatatat tgtccagtaa gtgatttgtc ggattgaagt
     4501 agaccttcta aatctccttg atatactaat tgaccacctt caacaccagc ctttggacca
     4561 atatcaataa tatgctctgc catctcaatt gtgtttttat catgttcaac tactattacc
     4621 gtattgccta aatctttaag tttctttatt gattttaaga tgtctatttt ttctgattca
     4681 tgtaatccag ctgttggctc atcaagcaca taaattaatg agtccatctc tgaatcgaga
     4741 tgtgaattta aaaatagtct ttgcatttcc ccaccactca aagttgacat ttctcggtat
     4801 aaggatagat gacctaatcg tgattctatt aaatgtcgag ttttggataa aatatcattt
     4861 acaagatttg taccaaatgt gtttaaatta ccttggtcta ttaagagttt aaggaaatca
     4921 tcaatttcta aaatcgtcat ttttccaagt tctccaatat gttttccgtt taaaagtact
     4981 ctacgtgctt cttcaccaat tttaaaacca ttgcaatctg aacaggcagt catagtataa
     5041 actccattta aatcttcgcc ccttttataa cgattgtgaa atacctttga taaagaggaa
     5101 ctttttttac cattttcata atgaccatag agtacttcat ttcttacttc ttcaggtaat
     5161 tgtaagaatt gtatgtctaa ataatcgttg aattttttag agaatactct caaatatcct
     5221 ggtgtaatcc ttgctctatc taagacttgt tttagggtat catgcttatc aaggactaat
     5281 ttttcaatat taatctcaaa atatgcacca tgtccagagc atttcataca cattccatct
     5341 gaagagttgt atgagaaata acttggtctt aatcgttctt cttcataccc acaattattg
     5401 caaattaaat tgtcgcttac aggagtatca cacatagtac acattatttg accttctact
     5461 gaatacaata gtgtaagcat gttgagaata ttggttcttg tgcctactgt tgaacgagga
     5521 ttactttggc gaataatatt ttgctgaaca gctatagtgg gagctaaacc ttgaatatta
     5581 tcgaacttat cctcactatc aatacctgca agtataccta aagattgcag atattgttta
     5641 cgtccttcct caaatacaat gtcaaacata aggctagatt tacctgagcc actgacacct
     5701 gttgcaacaa caagcttatc tttgggaata gaaacatcta tatttttaag attgtgtatc
     5761 tttgctccat taatttttat ttctttcatt ggttcacctc attttatcaa ttttatcaaa
     5821 ccgtaaaata gggatgtatt aatccttaat acaagaatag attaggtgag gttattaaaa
     5881 aggttctata ggtaaatata tatcgaccaa gtgtctattt tgagggtgat tgtgtgggtt
     5941 gttaagatat acttcaaaag ggaaggaggc acgtaatttg taattaccat ccgatagcca
     6001 ttcattatac ataaaatccc acgcatcact atattcagat tttgaaatat caaaatgtcc
     6061 cacaagatat tttccagaag gtattatcat attgccaaga ttgtcattct ctttgacaac
     6121 aatgttatct ggaatggtca tgcaaatact agtccttaat tgatgctcct gtgtaaattg
     6181 tggattatca tgataaatgg ctaatatttg tgtgttttca agtccaagta gattctgctc
     6241 acttgcatat gtgaataacc gttctaaaag ttttgggaaa gtactggtta gattaccata
     6301 tgaaccagta tatctagtgt aaatagtatt catattatca agttctagaa tttcaatttc
     6361 acctttaacc tctctagtat tcttacattt aggttttgat atgcttctat tgtactgaga
     6421 aattttatat ggttctttgc aattcttgct ataattattt ctatatttta taggacttat
     6481 tttatagtgg tttttaaatg ctctcgagaa aactgctgaa tcagtaaagc caaagtagtg
     6541 tgcaatatca gtaatggtca tgtcagttcg atgaataaga aaagcagttg cattttctag
     6601 ttttagccta tttacatact gtgccaaagt ttctttttct attgctttga agattctatg
     6661 aaaatgaaac tttgagaaac ctgctatatt agaaagttcg tcaagggaaa gaggttcatg
     6721 taaatgttct tctatatagt cttgcacttt gcaaatacga tttagatatt cttttttact
     6781 tttagcgttt gtcatagata gccctcctta ttgtgaaaaa tgtgtattaa ttaatttata
     6841 gtaatatagt aataaaattc tatgtttttg taatttatga taaataatat agagttcaat
     6901 tttttataat taataaaata tatttataac caacaaaaga tataagatgt cttatatttg
     6961 tctttgcata taagtatagt cttcgtaata attagtatac catagtgatt tatttttaat
     7021 tgtgtattta tataaataca caacttatga tatggacggg ttattatatg aataagctgt
     7081 aattaaataa attacaatat aaaccagtta aaacataagc tagagaatat tgtggaattt
     7141 attacctagc acaagagtat agggaagata aaataagatt aaaaaattac atactaaatt
     7201 taataaaata tttatattaa aaataatatt aagtttattg tttaaataaa aaagttaaat
     7261 ctacactatt aacttattac acttaaaact ttaaaatgaa aatatcaaaa aatacttttt
     7321 aataaaaata atcatttaat aagttattta gaaatctaaa aactttacaa caaattatct
     7381 atttaaattg gaggagtggg aatatgagta atagttttta tcaattaaat tcagcagtat
     7441 tatattttaa tgaaaaaaga gatataaata gaataagatt taatcactat gtaatgagaa
     7501 aagaaaatat acaaagatta aaaggaactc tgccaagagg acaatttatg atgacagtaa
     7561 ggaaagctgc atctgacctt gatgtatcta tatctacaat aagtagactt gtaaatgaat
     7621 ttatagattt aggtatattg agattaataa gtaggggagt aaaggggaat agttgttctg
     7681 tatatagtta tgtttgttct gaaaatagta atgcagaaat tgtagcaaag aatggtgata
     7741 ttttcaaaaa tatagagaaa tacattgatg taactattaa caataaccaa aaaacaatga
     7801 aatataaaca tagagatgag ttgttcaaga atagtaatac caacatatac agtcgtataa
     7861 gtgaagagag tatagacaaa gattatgatt tgataattca tactgggtat gaaacagaaa
     7921 atgagacaaa gaaaaaagaa ttattaaaaa ataatttaaa taaaaagaat ttaaataaaa
     7981 atacctttga aaattcggac gaagatttga ctgaagattc tacttataaa aatccagaaa
     8041 gtatacatga aattattata agaaaattaa atagtgaatc gggaaaaaat tttaaagctg
     8101 atttaagtat aactaaggca cttatagata aaagattaaa agaagggtat agtttagaag
     8161 atttttacaa ggttatagag gcaaaggtaa gaagatggag atatacagat atggaaatat
     8221 atatagaacc aacaattctg tttgggtatg aatttgagtc gtatttaagt gaggagagtt
     8281 ctttatctgc ttgtaattat ggtgatatgt gttatgagga tatgtattga gatatttatt
     8341 aagatattag ttgcttttta gttgtttaaa atatgcttat ataagtataa ctagtgtatt
     8401 aaaaatggaa ttgtctcaaa atagggagaa gcctcttttt aattatttaa atgatgaaaa
     8461 tatatttgat ttaatacaaa ggagagtgcc tcatgagcta gaaagctcat tttgagacac
     8521 ccactttttt taatatactg gagaaatata acccatttga atggcttttg taatagcctc
     8581 aactgatgat gaaacatcta gcttgtcata tatgtgttgt aaatgatttg ataatgttct
     8641 ttcacttata tacaattcat cagcaattat ttttcttttt ttaccactac agataagctg
     8701 taaaattctt ttctccatac tagttaaatc ttcaataatt acttcagtat tttcagatgg
     8761 aaaacaagtt cccccttgat agacttttaa taatgaatca attaaattat cagggctaat
     8821 attcttattt aggaatccat agatacctat ttttttagct tcatgtttat aaactgggag
     8881 gtcgtatcca gtcaagatta ttattttaat agttgaatta gtctctaata aattataagc
     8941 tacttttaag ccatcacatt cacttaagcc tctaaggttt atatccatta agacaatgtc
     9001 tatcttttta tcttgaatca tttctgatag actatggatg tcctgtgtgg aataaaactg
     9061 ttcgatttct tcgtattctt ctagtgcaat ggacaagctt ttagcaaaaa gcatatggtc
     9121 atcaactaac aaaatattta taagaaccat ctcctttcat agggattgta atctgtacac
     9181 aaactccatg aggtatatta tcagaaatac ttatcgttcc tcctagattt tctacttgtt
     9241 ctttaattga gataatccct ttgtgtttta ttgtatcaaa agtaatttta gttatatcta
     9301 aaggcgtttt aaaagatgta tttttcacac cattatcatt aatatttaat ttaattatat
     9361 ttttttctag tgctaaagct atccatgcat ggctaccatt tgaatgttta tatacgtttg
     9421 tcaataattc tttaatcagt cgatatatca atatatcata tggttcaact aagaataaag
     9481 tatcagaaca cttaaatgaa acacaaatgt ttttttgtgg aaaggatgaa gaaacacttt
     9541 caattaaatt ctgataattt tcttttactg ttaaattttt taaaataaca gggtgatagt
     9601 cttgcatttg ttttcttata catgtattta gattgttaag tgtttcatat ataatatctt
     9661 gaacttctgg gcgatatgac ttactcatca tgttttttat tgaaagcagg ttttgtaata
     9721 tatcatcatg taaaaaatta gaaaactctg tttttagttg ttcttcttgt tgtaattttt
     9781 ccaatgcaaa agtatacttg ctatttttaa ctaccttatt tttttcattt ctaaggttaa
     9841 actccaaaat aatgttacag aggaaaataa gcataaataa aacatttata gctattaata
     9901 ttatataaaa attcccactg gcaaatagta caaccaagct aattactaaa atacttataa
     9961 aaagaaatat agacctttgc tttatgttaa aaactgttga aataggaaaa ctatcatttt
    10021 ctttaagtgc aataccatga atactcataa aaaatactaa tacagttaga tagattccaa
    10081 aattaccaat atgatttgat attcttagtg taaaaaaata ataagtaagg aatcctataa
    10141 ttgttataaa taccgaaatc gatataggtt ttttttcact ttttatgaca aaagcgaccc
    10201 ttttccaatg ataaaaaata atatagaaaa agcttattat actaacaaca aactgtattc
    10261 cgtacataca agcatacgtt ctgttagaaa taaatgctcc tacaattgca ccaatgcagg
    10321 tgataagcaa taaaatgtct gttatcttac ggaacttgta tccactacct tgaaacaaaa
    10381 ataaaaaaat aaatttaata gagacatacg ctataactgt gcttaatatt ttaaaaataa
    10441 tagtgcctat tggtgtggct acaaatgata agagtaaata ccaactgtct agtgctagta
    10501 atccacaaaa caatgaaata atgttatttt ctcggacatt acaagaggtt atatatgtga
    10561 tgtctatgag taataatgtt gaaattatta agggaataat gctttggata ttcatataag
    10621 tttcagtgct tgtagatatt cccatgaaga gaagtgatag aatgtagttt cctaaaaata
    10681 gtatttttaa attaaaattt gtcaatctta tacacctcct tatgagttaa ttatattaat
    10741 tttatgacta aatcaaactt aagaatttat aattttaaat tatatcaaat acttataaat
    10801 gaagtatacc aaatcgtcac acctttaagc ttgacgattt ggtaaatatt atttttttaa
    10861 tacaatttta caacaggatt taatagtaag tatgtaatat atcaaataca ctacagaagt
    10921 taagaaaatt gcaataatca caggtatgta ttgtgaaagt gaatttccaa gtaaattctg
    10981 catcaaagca gtcttataaa ccaaggtagc aaatatacaa tccactatac caagtaaaaa
    11041 cggaatatta aaaaatggca atacttgttt tttaataatt tttttaattt gtatattagt
    11101 ataacccatt ttctgtaaaa ttttataatc agatgttgaa tctgaaattg ctgatatatt
    11161 gttaaagtat aaaatacttc cagaagcaat aaagaataat actacaagaa atccgattaa
    11221 tagaaatgta gaactattaa ttgataataa atcatttatc ctgtgagaat atccttgtaa
    11281 ataagggctt ccatctaaat aatctgaaat attactatat aaattttcat tatttttaat
    11341 tgatttacca ttaatactta aaatgcttgt cattggtaat ttattttgtg ctatttgctc
    11401 atatatacta tcattaacga taagtgttcc aacgctatta gcaaaagaaa tagcattgtt
    11461 taatgatgtt tcccttacag ttacaggtgt ttctttacca ttgataagta atggataaat
    11521 cgttccattt tcactatatc tatcattgtt tggttgatat ttcattaaaa tacattcatt
    11581 atcccttaaa gggataagtt gttttattag ctttgttcga ttttgtgcct ttaacaaagt
    11641 ataataatca gaaaaagata tacattcaaa tcctgattct cttgaaataa cttctttaga
    11701 agaattacga ttagataaat tattcttaga tgtaccaatt tcatattcaa gtggaaggtc
    11761 ttttgatgaa gattttacct tataaatatt tgtctgtgta aaaataatat cgtctgtatt
    11821 agacatgtat tgactgacaa tttgttttac atcttctatt tgtttatttt tttctactct
    11881 aaattctatc tctgaaggtg caatacgtga aacggatgca atagggtagt ataaagacag
    11941 tgccatgaca cttgaaactg tcaaagtagc agcagagagt aatgtaagca taattagagt
    12001 tttagagttt gaacggatac gatagataaa gtttggaata gttattatct ttgtacctgt
    12061 atagaaagct cgtttatttt ttttactctt ttgcataata tatggaagaa aggaattgat
    12121 aaagagaact gtaccaatta caatgataat catagttatc aagcccattt gataaaatcc
    12181 aatacttatc catactgact tagctccacg aagaatatct agtgataaag aatacccact
    12241 taaaatcata ataaacccca taaaggctgg aatttttctg aacttcaatt ttttttctgt
    12301 gcttttttca tatcgtatca attttataag tgaagtctta taaagaaatt taccatttga
    12361 tatagctaag actaaaacaa ctaatgctat aaagataatt gttttgttta ttgcatttat
    12421 attaaaaaat ggtatctgtg aattatcaat tgataaatta agtagtgctg taattccaaa
    12481 gacaataccc ttatgtgcca atgctcctaa taccaatcca ataataaaag ctccactaca
    12541 aattaatata ttttcagagg taagtagtga taagattgta aacttacgat atccaagtaa
    12601 agcatatatg cctaattctt ttgtacgacg tcttaagaag aatcgatttg agtatgccat
    12661 atagaagatg acaaatgcca tcaaaaatat cgaaataaga ttacacatag attcaactct
    12721 accatcagtt gaaatttttt ctagcattat cttattaaca gaaaatgaag taaatgcgaa
    12781 aaatacagta ataacaaaag agactgaaag tagatataga gaatagaatg aaaagttttt
    12841 tttaagattc tttattgcca tataaaaaat gttcattaaa ttatcctcct atcttttagt
    12901 atccaatttt gcaatttctt gaataatcaa ctcatagaat gtatgcctat tattctgttg
    12961 tttatttaat tctttaatga tatgaccatc tttaaaaatc aaaattctat ttgcaaaact
    13021 tgctgcatag acatcatgtg ttaccattaa aattgttgtt ttcaactctt catttgcttc
    13081 ttgtagtgag tggagtagtt cagtagctga attagaatcc aaagctcctg ttggttcatc
    13141 tgcaaagaga atactaggat gttttactat tgctctaact gctgaagctc tctgtctttg
    13201 cccaccagat aattcatttg ggtatttgtt taattgagaa gctatgccaa atcgttcagc
    13261 tagactcttt accatttttt ctattttatc tggacttagt ttttgaagtg taagaggtac
    13321 tgaaatattt tcgaaaattg taaggctatc tagtaaaaaa tactcttgaa agataaagcc
    13381 taaattatcc ttacgaaaat cagcagcact agtgtcatta agcgatttca aatttatatt
    13441 atttatgaaa atgtctccac ttgttggttt atcaattgta gataatacat taagaagagt
    13501 tgttttccca gaaccagaag ctcccattat acctataaat tcaccttctg atacattaaa
    13561 ggatacctta tctaaactcg gctgttgtcc tttataagtt ttagttatgc tatttacttt
    13621 taatattgta ttcattgtat atgccccttt catcttgata tagtcaaatt atatattaac
    13681 cttattgttc aatcatgagt aaatcacgca ataattctac acaaattact tatatcacaa
    13741 tatattgttt aaaatctaac tttattaggt ttgggatgat ggacaagatt ttaatttaaa
    13801 agagtgcata gacatatata atggcataat gtggatttaa attaaaaaaa taaaataaag
    13861 ccataaatat tgaatttttc aatatttatg gctttattgc tttgtaataa ttaatagaat
    13921 atattttttt attaatcatc tatttcaaat tctagtattt caccagtaat tgcattaatt
    13981 gttatctcat acttagtttt tccttttatt atttcacctt catattcttt gtcatcttca
    14041 tcatattcaa attttactat tttcccacca tgtactttat ctagcattat tgatttagct
    14101 ttttcagcac caataataga attgctgtca ttagtattag agctattgtt gtgattatta
    14161 tcattatcat caaagtcatc atcagaatcc ttgtccatat caatatctac aattcttcca
    14221 tcaaatgcac ttatctctaa ttcatatgtt ttcttatttt ttattacttt agcctcatat
    14281 tcattatctt cagcatcata tttacaatag attagattac cattaggaac agcatctagt
    14341 gctatagact tagctttatc caatgatata ttttgtgtac ctaaattatt atcatttaag
    14401 tcatcatctt tttcaatttc tattatttta ccagagtagg catcaatttc tatctcatat
    14461 aggacattat cctttataac tttgcagtcg tatttattat catcttcatc atagtaacaa
    14521 actaaaatat tacttcctgg aagggcttgc tttaatattt ttttagcctg tattaaagat
    14581 atttttttag ttttatttct tatgttcaca ttttttagtt ttaaaatatc ttcaaacgct
    14641 ttttgattat atcctcctcc tacttgtgtt atttcatcta tatctttatt tttaataaat
    14701 tccttttgac tatttacaag gctaccatta gatatcatta taggagaatt ttctttagca
    14761 gctagtactc ctattgataa tgaatctact agtgcatttt cttggttaat accatcttta
    14821 gctacaaata cattatttat ttttttatct ttataaaatt tatcaataat ttttgcatta
    14881 gtattatttc tatctgaacc actgattctt tctctgtttg gatatttttt tactgcatta
    14941 tcagataaaa tcctatttcc tccaactatg taagattttt ttatagacct attctttaag
    15001 aaatttatac tactatcatt gctgttttca tctgatacta atattatagg catattatgt
    15061 attgctgctg gagaagctat actaactgca tcagataatc cctttagtcc atttacaatt
    15121 gcaacatttt ctatcttttt tatcttatct atttctttaa gtacatttgt agacatttca
    15181 tatatattgt ttcctactat tttatttatt tttatattaa gtgattttat ttcattttct
    15241 actttagaag aaatactatt tccactatct acaatataca cattatctac accaagcctt
    15301 ttaagttcag atttagttga attttcgata ctgttgttgt ttactaaaag tataggtgcg
    15361 ttctttagct tagcaaatgg atttgcacaa agcgcacttt gaatagagtt accattaact
    15421 ataatggctg tttttgattg aacccatccc aatttactta cttctactgc tgtttcatat
    15481 cttgttttac ctattaatct ttcttgcttt gtagtattgt ctgcaaaaat tgctgaagaa
    15541 ctagaaaaaa acatcatacc cgatattaat atagctaaat ttttcttatt catattttta
    15601 cctcacaatc tttaaaaatt tgttatttgg catacttaat aaaatataat cttaaaatca
    15661 aattgatact gaatttaaag ttaaaaaatc aataaataag atttctttaa tacaaaattt
    15721 tcctttttat atctaacatt gtatcataaa tataaagagg aaaaaacttt ttaaaaaggg
    15781 tatacactat tcatttttta tcttataaat aagtctttac cactgtttta tatattttaa
    15841 aataggcaca aaacctattt ctaaatcttt tccaaatctt ttaggtgaaa tttcttttta
    15901 aacatatcta aaaagtattt gtagtaatgt taatagactg aatttcttaa agtatatatt
    15961 tctttggatg ttggagtttg tgaaatttga gaattggaat gtgaaaggaa catgcaatgg
    16021 attaatatta aaggactgta tttttataat atttgtctta aaaataaaaa aatctattga
    16081 cttgaagtta actccaagta gtaaagtata attatacaag aattatatac ttgtaagaat
    16141 agttaaagta gagaaataaa acaaacaagt tttaaagggg gaagtaaaac aatggaggca
    16201 aaaaaattag gatttggatt aatgcgttta ccaataaaag atgaaaatga tgttacaact
    16261 attgatatgg aacatttaaa taaaatggta gacacttttt tagaaagagg atttacatat
    16321 tttgatacag cttatgtata tcacatggga aaaagtgaga tagcacttag agaatctcta
    16381 gtaaaaagac ataaaagaga gagttttact gtagctacaa aacttccatt aatggcttta
    16441 aagaaaaaag aagagcaaga aaccattttt aatgagcaat tagaaaaatg tggtgtagac
    16501 tattttgatt actatctttt acacaatata ggtgtgtcac attatgaagt agctaaaaaa
    16561 tttgatagct ttaagtttat tgaagaaaag aaaaaagaag gaaaaataaa gaatattggt
    16621 ttttcttttc atgatagtgc agaattattg gataaagtct tgagtgagca tccagaagta
    16681 gacttcgtgc aattacagat taattattta gattgggata atgaaagcat acaatctaga
    16741 aaatgctacg aggtggcaga gaagcataat aagcctgtaa ttgttatgga gccagtaaaa
    16801 ggagggacac ttgcaaagat accagaaaaa gcagagaaat tattgaacga ctacaatcca
    16861 gatatgtcaa tttcatcttg ggcaatacgt tttgcagcaa gtaaagataa tgttatgatg
    16921 gtcttaagtg ggatgtcaaa tatggaacaa ttattagaca atacaggata tatgcaaaat
    16981 ttcaaaccat tcgtacaaga ggaatataat attatagatg aagcagtaga gattataaac
    17041 gaatcaattt tagttccttg tactgcctgt cagtattgtg tagaaggctg tccaaaaaat
    17101 atcgctattc caaactattt tgcgttgtac aatgcagaaa aacaagctct taatactgga
    17161 ttttcaacac agatggttta ctataataat tatactaaaa cttatggaaa ggcctcagat
    17221 tgcattgaat gtaaacaatg tgaagaaagt tgtccacaac acattaaaat tatagatgct
    17281 ttaaagaatg tagcagaaat ctttgagaaa taattcaatc tatacaatat caagagggga
    17341 agatatgtat gacgatttca gaagttagta aaaaatacga gctttcagca gatactttac
    17401 gatattatga acgaattggt ttaataccac ctgtaaatag aaataaaagt ggaattaggt
    17461 cttttacaga agaagattgt gagtgggtta atttcataaa gtgtatgaga ggtgcaggtc
    17521 tttctattga gacattaatt gagtatgtag ctatgtttca acaagggagc agtactatca
    17581 aagctagaaa agaactttta atagagcaga gaaaccaact agcaaaaaga atagaagaaa
    17641 tgcaaaaaac tttggaacga ttaaatttta agattgatag atatgaagaa ggtattattg
    17701 agaaagaaaa agtactaaag agcagtagaa ataagaaaat ggaggaagtg tcttatgagt
    17761 aaaaatgtat taatagtatc agctagtcct agaaaaggtg gaaattctga cttattgtgt
    17821 gaccagttta tacaaggggc attagaatct aaaaatcaag tggagaagat atttttaaaa
    17881 gataaagata ttaactattg tactggatgt ggtgcttgtt atgaaaaaga agcaagttgt
    17941 tcacagacag atgacatggg agaaattctt gaaaaaatga ttgaggcaga tgtgattgtt
    18001 ttagcgactc cagtttattt ttatactatg aatgcgcaaa tgaaaactat gattgataga
    18061 acttgttcta gatatactga aatttgtgat aaagatttct attttatcgt ttcagcagca
    18121 gatgaaaatg tggctgatat ggaaagaaca atagaaggat ttagaggatt tacatcatgt
    18181 ctaaatggtt ctaaggaaaa aggtatagta tatggtgtag gggcttggaa tgtaggtgat
    18241 attaagggaa gtaaggcaat gaaacaagca tatgaaatgg gtaaattggt gtagtatgtt
    18301 tgtataaaat tgtataaaat cgaatatatt cttatcattt caacaataaa aagaggctta
    18361 tctatatttt gagacagcct ctttacgcta tatcatttat ttttattcta tatccttact
    18421 gtatccttta tttttttatg cctatttaag tatctgttgt tacttatata ttttaataat
    18481 tacccttaaa aaactataca gattcctgca taatattatt ttctttagta attaattttt
    18541 ctaggttttt ccaatcgatt tcttccatat ttgcaaaata atgaccactt cctatgcaag
    18601 tcaggtaaaa ctttccatct aattcaaacg aagaacctcc tatacacttt gaattacatt
    18661 ttttacaagt acgagtttta gttatactat ctttaatagt tttagagcat tcctccacag
    18721 catctttata tgtatttaga tgaaaaagat ttgctttaac tctccatttt ttttcattac
    18781 attcaatagt aaataagcta cgttttggtt tcttagttga atatactatt ttgtaagaac
    18841 gagcacctgt ctttggttca attttacaat ctaatgattt accaagttca tgtaaatgac
    18901 atgcaatatc ataatcttta ccacttagta cttctaacat ttcttctgtt gtttctgctg
    18961 ttaattcact tttgctcata ttaaaaaaat ccccctatat aaataataat ttcatatgaa
    19021 aataattaat ataagtatat taaataaaac tattaaaatc aataaaatta ctgcaatctt
    19081 acattattgg gcatagaatg agagctaatg atttattaga aataagactt aatagtatga
    19141 aaatttactt agacgtggta caattctata aaagatttaa aactaggaaa ggagaaagtt
    19201 gttgagatac ggccaatata acaatttatt tataagttgt aaatttatta tgagattaat
    19261 tagattgtta agtaagacta tcaaaaggta gttttttgga ttttcagcaa cagtggtaga
    19321 tattgataat tttttggtta ttattatatt tagctgttat attttcaata ggctctatga
    19381 taactagggt aaataaggta tgggtaaagg ttttattatt tataatagtg ggattgtcaa
    19441 ctatagttct ttcatatgta ataacttgat tatgcaaaat tctatgaagc aggaaccctg
    19501 tgactttagt catgggaggt tcagaattta aggttaaaaa ataactttaa tctgtaaatt
    19561 ctcaaaacaa gaattctcat gttggtaaat ttctagatat gagaaaaaaa ttgacatcat
    19621 aaaaagtaat tttagagcgt ttttttacga aaaaattcag aggtaactat tttgaatgac
    19681 ttcacaaaat tttatatata aatgaagttt ggcatgttaa ttgctttaat aaaagttaac
    19741 aagacacaaa taaacataat catctaagta ttgattattt gaagttttag atttgaaatt
    19801 taatctaaag gttcgtatta atattggtgt ttaggaaatt ttattttcct gtaaatataa
    19861 cttaataata ggaggtgaag tcatgtcaaa agtagtaata gctacagaga aagctccagg
    19921 agcaattgga ccatattctc aagcaattca agttggaaac atagtttcta catcaggtca
    19981 acttccaata gatatgaaga ctggcgagtt ggtatctgac ccaaaagaag caacaaaagc
    20041 ttctttagat aatgtaaaag caatattaga acaagctggt acaagtatgg ataaaatatt
    20101 taaaactaca gtattcgtaa aagatttaaa ggattttaat gatattaatg aagtgtatgc
    20161 tacatatttt aaagaaaatc caccagcaag gtcatgtgta caagttgctc aattaccaaa
    20221 agatgcagtt ttagaaattg aagcaatggc agtattataa gtattataac aataaaacca
    20281 ataacaaaac taatgaaaat taaaaactaa taaaaaaagt ctacaattaa aaaagtttat
    20341 attaaaaaag tctatataaa aatctatcta aatatttaac tattgagctt ttctcatata
    20401 atgtatattt ttatgtcaaa aatatatttg ttatatgagg aaagcttttt tttattattg
    20461 tattgtgatt tattttattg tctgcatatt ataattaagg tcactatata aaacaaactt
    20521 tttataaatt aatattgtgt gttttaacgc aaatatataa ataagtaatg ttttatatat
    20581 attttaaaga gtgactatat gttaaaatat agctaaactg ataatttggt aaattaaatt
    20641 ctaatggggg aaactatgtt aagtaaatta aaagaatttc aacaagaaat gataaaatac
    20701 actgaaacag tagctagtgt gttagatgta gatattgaaa tagttgatga tagattaatc
    20761 aggattagtg gcacaggtct gtataaatca aagataaatg aaagcgttgt tacagaagga
    20821 tttatttatg acaatgttat acagacaggt caggaattag tcgtattgga tatatgtgat
    20881 aatcagcttt gtatagaatg ttcccattac atgaaatgcc taaataaagt aataatagca
    20941 gttccaataa agtataataa tcgaactata ggagtaattg gtgctattag tacagataaa
    21001 actaaaaaag tagaaataag tgcaaaaata gataattatt taaaatttgt caatcatata
    21061 tgcgatttaa tttctatgaa gatagaagaa catgaagcgt caaaaaatag cagtagaaaa
    21121 atggatatga tgatagaaat aattgaaaat gttgaaaaag gcgttataat actagacata
    21181 aatagcaaaa tatcttatat aaacaatata gctctaaaaa aattagatat agataagaat
    21241 ataatagaaa atatagtaaa tatagtatct gttgaatctt cctcaaatgg acatgaatta
    21301 ctagaaattg atattgataa caaaatttat aatataaatg ccaaaataat tccggtatat
    21361 ccatatataa atcaatacga caaaataata atatttgaca agacatatat caatcataaa
    21421 ggacatggaa aagtcaatag tggctgggga aatagtgata tagagtctat aattggaaat
    21481 tcagagtcaa tgttaaaagt aaaagagaga actaagaaat tagcaaagtc aaattcaact
    21541 gtacttataa ctggggaaag tggtacgggt aaagagctaa ttgctagagc tatacatgct
    21601 gaaggtagta gatggaacaa gccatttatt gcgataaact gtgctgctat tccagaaaat
    21661 ctccttgaaa gtgaactatt tggctatata aaaggtgcat ttagtggagc tagtagtgga
    21721 ggtaaagtag gtaaatttga acttgcaaat gaaggtgtta tatttcttga tgaaatagga
    21781 gatttgtcaa tgcctttaca agcaaagtta cttagagttt tacaagagag aaaatttgcg
    21841 agaattgggt caaataaact tatagattta gatataagag tgattgctgc tacaaataaa
    21901 aacttattga aattagtaaa tgaaggtaaa tttagggatg atttatacta tagattaaat
    21961 gtaattccta taaatttgcc acctcttagg gaaagaaagg atgatattga ggcaataatg
    22021 atgaagtttg cttctaaata ttctttagaa ttaggtattc agttaagtaa aatagaagaa
    22081 aatgtaatga atatgttgat taactataat tggccgggaa atataaggga actggaaaat
    22141 gcagttgaat atatgatgaa tttggttgga gatgatggaa taatatataa agatatgctt
    22201 ccattggata ttttaaatta ttataatatt aatggaaatg tttgtaaaaa taaggatata
    22261 aatataatat ttgaagatga catagttgga gggatagtag agaatcaaga aaggatactt
    22321 tcaattaaag aattagagtt gacttacata aacaagttgt tgaataagta tggaagagat
    22381 acaaagacga agaagaagat agcaaaagac ttaggcatag gtttggctac actatataga
    22441 aaattggaag aggaacagta gtaatataat attaaaatga taggtatttg tgtgttttat
    22501 agataggtat ttttatttta tggtggaggt gttattatgc aatatgcgat agagctatat
    22561 tatgataaaa aaacggaaaa gcaacttttt gatttatcta aaaaaatcgc tgatgaaaaa
    22621 attagtacga agtacttaga atggaaaaca agacctcatc ttactttggc ttgtttcaat
    22681 gatgtggatg aggcttgttg catagacaag ctaaaggggt ttgcacaaaa ccataaggta
    22741 atacctgctt acattggttc agtaggcatg tttaatgata ctaaaacggt gtttgtatca
    22801 cctataatga atagtagtat gtatcagttt cagagagaat tatatgaggt actacaagat
    22861 tttgattcaa atggttggga atggtaccac cccaatcgtt gggtacccca ttgcacaata
    22921 gcacttactg gagatgatga aagtgaggct ttttttcggg caagtgatgt attactgcgt
    22981 gagttcaaaa aaataagtgg tgagtttgtc tcaataggat tggttaaaat tacatttcct
    23041 gtggaagaaa tttttacagt gaatatgaat aggtaacttt aactctgttg tggatattat
    23101 gaatattttg catagtgcat ttcatcagat aaatgcgaag ttgcgaattt cgacagctca
    23161 atagtcgcaa gtagggcaca atggtacaca tattgcactt ttgcttattg gggggacgcc
    23221 catgtaaact tctgtaagaa gctatgaaaa gatttaagcc attttggagg ctttgggtaa
    23281 taaagaatat aaagtatata aatttataga gtttatacgt aagaaggtta tagccatata
    23341 tttatagaaa ataaaagtag caatatatct gacaaaacaa tagacatata aaaaataaga
    23401 gcataataaa ggaagaatta aaataaataa acgatttctc gaattgataa ttctcgtatt
    23461 gataaaaatt tcccaatatg agaatttttt cttttataaa tcttattttt cacatttaat
    23521 atttataaaa ttcaaagaaa ttttataaat atatgtaatt ccaacataaa aaaattgttt
    23581 ttagaataaa taatattggc atagtaattg cttcactata agacacaaag ataaaaaatt
    23641 aaaatgacat cgaaaaattt gaaaggggaa tataaaataa atgagagaaa taaaatggaa
    23701 attaaataat ttacctaaat cagaggaaaa agaaaaagga atcgaatttt taaaagatga
    23761 agagattgca aaggctaaag cttttcatga aagctttcca caatatgaaa agactccttt
    23821 agttaaatta gataacttag caaaatcact gggcgtaagc ggaatatatg ttaaagacga
    23881 atcttataga tttggcttaa acgcatttaa agtattaggt ggttcttact ctatgggtag
    23941 atacttagct caaagattaa atacagatat atctgagtta gggtatgata aattaacttc
    24001 aaaagaaata aaagaaaaat taggagaaat aactttcttt acagccacag atggaaatca
    24061 tggtagagga gttgcttgga cagctaataa attaggtcaa aaatctgttg tattaatgcc
    24121 aaaaggttct tctgaattta gattgaataa aatcaaagga gaaggtgcag atgctagtat
    24181 aacagactta aactacgatg atgcagtaag attagctaat gattatgcag aagctgatga
    24241 ccacggagta atggttcaag atacagcatg ggatggatat gaagaaatac cagcatggat
    24301 aatgcaagga tatggaacta tggctaaaga agctatagag caattaaaag aatatggagt
    24361 agatagacca acacatgtat tcgtgcaagc aggagttggt tcacttgctg gtgcagttca
    24421 agggtatgtt gcttcgatat atgatgaatg tccaataaca gtagtagtag aggctgatga
    24481 agctgattgt tactataaat cagcagaagc tggagatgga aagccaagat ttgttggagg
    24541 agatatgcca actataatgg cagggcttgc ttgtggagag ccaaatacta tagggtttga
    24601 agtgttaaaa aattattcat cagcattcgt ttcagcacca gactgggtgt ctgctaaagg
    24661 tatgagaata ttaggaaatc ctctaagagg agatgagaaa gtaatatctg gagaatctgg
    24721 agcagttaca actggagcat tagtttcaat ccttggaagt gaagatttga aagacttaag
    24781 agaagctttg aaactagatg agaactctaa ggtattatta ataagtacag aaggagatac
    24841 tgaccctgat aaatatagag atatagtttg gaatggtgac tgccaaagta aataaaagac
    24901 ttaaggaaaa gaaaaactaa acaaataaaa gaaaaactaa agcaaatgaa aaattcaaaa
    24961 taaaaactaa ataaaattaa ataaaaccaa ataaaattaa aataaagtct aaataaaaat
    25021 ttaataaata aaatataaag aaataaaaaa tcaatagatt aagtgaaatg ctaataaatt
    25081 aaaaaagtga aatatcaaaa taaaacgtca acaaattaaa aattatatgg gggaatatca
    25141 aaatgttaaa tgaaacaaga aaaaaacaat taactgaagt atgccaagac ttaataagaa
    25201 atgctagtta ttcaggtcaa gaagaaaacg tagtaaaagc tatagaagag aattttgctt
    25261 tactaggttt tgatagttgg tcaagagata gatatggaaa tatagtagga tgtataaaag
    25321 gaaataaacc aggtaaaaaa atattatttg acgggcatat agatacagtt ccagttccag
    25381 atgcaagtaa atggagtgtt cctccttttg aagcgagaat agtagatggg aaaatatatg
    25441 gtagaggaac ttctgatatg aaggggcaag taagtgctat gatggctgct gtttcatact
    25501 ttgcagaaga tactaacaaa gattttgaag gtgaattgta cgtagcaggt gttgttcatg
    25561 aagaaatatt tgaaggagtt tcagctagag agataagtaa ggctgttcaa cctgattatg
    25621 ttgtaatagg tgaatcttca gaattaaact taaagatagg ccaaagaggt agaggagaaa
    25681 tagtagtaga aacttttggt aaaccagctc actctgctaa tccagaaaaa ggtataaatg
    25741 cagtttataa aatggctaat gtaatccaaa agatacaaca attagtacct ccaactcatc
    25801 cagttttagg tgatggaata ttagttttaa cagatataaa atcttctcca tatccaggag
    25861 catcagtagt gccagattat tgtaaagcta cttttgacag aagattatta gttggagaaa
    25921 caagagaagg cgtattagct ccaatacaag ctttattaga tgaaatgatg aaagaagacc
    25981 cagaattaaa tgcaaaagtt tcttatgcag ttgaaaaagc tgattgctat acaggaaata
    26041 ctatagaaag tgaaagattc ttcccaggtt ggttatatga cgaagaagat gaatttgttc
    26101 aagcagcata taagggacta aaagaagctg gaatagattc agaaataact caatactcat
    26161 tctgtacgaa tggaagtcac tatgcaggtg aggctggaat aagaactata ggatttggtc
    26221 cttctaagga aaatcttgct catacaatag atgagtatat agaattagac caattattta
    26281 aaggtgcaga aggatattat ggtatattaa aatcagttta taaaaaataa ttaggagatg
    26341 gttttgtgag aactttaata aaacatgggc ttatagtaga tggaaataaa acaccagcat
    26401 atgaaggaga tattttaata gaaaacgaga aaatattaaa aatatcacag gatttaaacg
    26461 aagaagctga caaagttata gatgcaaagg gtagagttat atgtccaggt ttcatagata
    26521 ctcatagcca ttcagaccta gttattttag ttaatccata taatgaggta aagattagac
    26581 agggaattac aacagaagtg ttgggccaag atggtatatc tatggcccct ctacctcaag
    26641 agcatatatc ttcttggaga aaaaatttag caggtctaga tggtgaaagt gatgaaattg
    26701 attggaaata tgaaacaaca gagaattatt taaaaatgat ggattacaat ggggtaggtt
    26761 taaatgaaac ttatttagta cctcatggaa acgttagaat ggaagcaatg ggacttgaag
    26821 atagacctgc tactaaagaa gaaattcaaa aaatgtgtga aatcacagag agagaattaa
    26881 aagcaggagc tataggattg tctactggac ttatatatat accttgtgca tactcattga
    26941 ctgaagaaat tatagagatg tgtaaggtag tagcaaaata cgacggagta tttgtagttc
    27001 accaaagaag tgaagcagat acaattttaa catcaatgga agaaatcata gaaattggaa
    27061 aacaatcagg agtaaaagtt catttttcac attttaaggt ttgtggtaaa acaaactgga
    27121 agtatatacc acaagttata gagcttttag aaaaagcaga aaaggaaggc ataagagtat
    27181 cttttgacca atatccatat gcagcaggaa gtacaatgct tggagtagtt ttacctcctt
    27241 gggcacactc tggaggaact gataagttaa tagaaagatt aagtgatgaa aacgaaagag
    27301 caaaaatgaa aaaagatata gcaaatggaa tagaaggatg ggataatttt atagaatttg
    27361 cgggaataga ccaaatattt gtaacaagtg taaaaacaga aaagaataaa gatacaattg
    27421 gtaagagctt attagaaatt ggaaaaatga gaggaaaaga cccattagat gcaacgtttg
    27481 atttattaaa agaagaagaa aatgcagttg gaatggtaga tttctatgga ttagaagaac
    27541 atataatagg atttatgaaa agagacgagc aaaatgtgtg tacagatggt cttttagcag
    27601 gtaaaccaca tccaagagct tatggttctt tccctaaaat actaggaaga tatgttagag
    27661 agttaaatgt actaacaatc gaagaagctg tttataaaat gactaaaaaa gcagctacaa
    27721 gctttagtat aaaagataga ggcgaattaa aagaaggata ttttgcagat atagttatat
    27781 ttgacaagga tacagttagt ggttgtgatg attttatcaa ctctatgcag taccctacag
    27841 gtatagatta tgtaattata aatggaaatt gtgttgttga agaaggaaaa tacaataaaa
    27901 ttaaagcagg aaaagtactt aaaaattaaa tcttagcttt aaggaggaat aaatttatga
    27961 ttttagtggg gaatgggagg ctaataagcc aagattcttt aaatccctat atggaagatg
    28021 gatgtgtagt aataaatgat aatctaattg aagatatagg tactacagaa gaaatgaaag
    28081 ctaaatatcc agtacatgaa tttatagatg caggcaagaa aataatcatg cctggtctaa
    28141 taaattcaca tatgcatata tacagctcat ttgctagagg aatggcagtt ccaggaaaac
    28201 cttctgagaa ttttttagag atactaaaca atttatggtg gagacttgac aaacagctta
    28261 cactggaaga tacaaaatac agtgcatatg caacgtatat agaatgtata aaaaatggtg
    28321 taacaacagt ttatgaccat catgcaagtc caaatgcaat tgaaggcagt ttgtttacaa
    28381 tatcagatgt tgcaaaggat ttgggaatta gaacgtgtct ttgttatgaa gtgtctgata
    28441 gagatggcgg aaatacaatc caacaaggta tcaatgaaaa cgtaaacttt ataaaatacg
    28501 ctcaaaaaga tgatacagac atgataaaag gaatgtttgg acttcatgct gcatttacac
    28561 tatctgatga tacattaaat aaatgtgctt cggagatggc tggtcttgat acaggttacc
    28621 atgtacatgt tgctgaagga ctagcagact tacaatataa tgtagataaa tatggaaaaa
    28681 gggttttaga aagattcaat gattttggag tactaaaaga aaaaacaata gcagttcatt
    28741 gtatatatat aagtacagat gaaatcagcc ttttaaataa aacaaataca tttgtagttc
    28801 ataatccaga atccaatatg ggaaatgcag tgggatgttc tcctgcaata gagctattaa
    28861 aaaggggagt tacagtagga ttaggaactg atggatatac tagtgatatg tttgaatcat
    28921 taaaagttgc caatataatt cacaaacatc acttaaaaga cccaagagtt ggatttgaag
    28981 aatcacaaac aatgttattt gataacaata gaaaaatagc taaagcttat tataaaaatg
    29041 acctaggagt acttaaaaaa ggtgcttatg cagatgttat agtagttgat tatataccac
    29101 atactccatt gacagaaaac acaatagggg gacacactat ttttggaatg agtggaagaa
    29161 gcgtagatac aactataata aatggaaaag tacttatgaa agatagaaag ttaataaaca
    29221 tagatgaaga aagtattcta gcaaaaagta gagaattaag tcaaaatcta tggaatagaa
    29281 tttaaataat tgattatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg
    29341 aggttcagat taatatacaa gtaagaaaca agaatttaga tgttaaaagc agagattaaa
    29401 caaaaatagg aatttaaatg aagtatataa ataaaaaata tacggaatag aatattaaat
    29461 taaatataga agggagaccc aaaatgtctc aagataaaag ccctaaaata gctccagtag
    29521 atgaaaggtt acctgtatcc aaatcttggg tattttcact acagcacgtt ttagcaatgt
    29581 gtgcaggagc agtagcagtt cctatgatga taggaaatgc tgcacgacta gatcatgcta
    29641 gtattgtttt tttaataaat gcttgcttgt ttatggcagg tataggaaca ctcatacaaa
    29701 gtgttggtat aggaaatatt gtaggtgcta aaataccagt aatagaagga actagttttg
    29761 ccgctgtggc aggtatgaca gcaatagcct catcatatag tgattcacaa gttgcaatac
    29821 agacagtatt tggttctgta atagtagctg gattgttttg tatattactt gctccagtat
    29881 ttgaaaaatt aataagattt ttccctagtg tagttactgg aacagttgtt atggtaatag
    29941 gtatttcact atttccagtt gggataaaat ggataactga caacaaattg gaagcggttg
    30001 aaccaaactc attagcatta gctttagcag tattggcaat aactttactt tcatttaaat
    30061 tcttaaaagg aatttggaat agcacagcta tattggtagg tatagtactt ggtacactac
    30121 tttccatggc agttggaatg gcagatttta gtgcagtgaa tggagttggt tggcttaact
    30181 taaatgtacc tttaaaattt ggaatgccta gatttgaacc atctgcaata ctatcattgt
    30241 gtttgattat gttggtactt atgacggaat cagttggtaa tatgatagct attcatgaaa
    30301 tggtagacaa agaagtaaca ggtaaaaata ttaaaagagg tctatttgga gatggaatat
    30361 ctacagcatt atctggaata ttcaattcat ttccaataac tccattcggt caaaatacag
    30421 gaatagtagg tcttacaggt ataaaatcaa gatttatagc agtttacgca ggggttatat
    30481 tgctagtact tggattctta ccaaaatttg cagcaataat aggagcaata cctaagcctg
    30541 ttcttggagg cgtaggattt gcaatgtttg gaatggtaat ggttggagga gtaaaaactt
    30601 taagcaaagt gaaatttgat ggaactaaaa atggggttat agtagcagtt agtgttggtt
    30661 tgtctatgat accactaaca aatcctgatt tctatcataa tttcccaact tgggtacaaa
    30721 caatattcca tagtggaata acaacaggta gtttatcagc agtgctttta aacatattct
    30781 tcaatgaact aggttctaaa aaagaaaaaa ataataaagt tgaagaagca aagtaaaaat
    30841 aaaacaagta agtaaaaatc tatattgatt aaaagttatt aaacattaat aaaaataatt
    30901 cagagatatt aataattcaa caaaactatc ttatatttta aataatataa aaaagtaaat
    30961 aaataacgaa cacaaataaa agaatacaat ctaaatatta aaaatattta atttaaggga
    31021 gagataataa tatggctaat ttaaaagtta atttatatgg aatggaattt ttaaacccaa
    31081 ttatgacagc ggcaggtcct ggagcaaaag atggtgctct ctgtcaagag gcagcaaaag
    31141 gtggagcagg agctatatgt acaaaaacta tatccgtatt accagctgat gtaccaagac
    31201 catgtatggc aaaaactaat agtggttttt taaacacaga attatggtca gagcttccaa
    31261 aagagcaatg gatagaggaa gaatacaaaa aggtaaaaga aacaggtctt ccagtaatag
    31321 taagtatggg atattcagca gagcaaatag ctgaggttgc tcctttagta aaaccttatg
    31381 cagatgcagt tgaattatct actcattatg taggtacaga tgttgcacca atagtaaatg
    31441 ctttaaaagc tgcaaaagct gttttagatg taccagtatt tatgaaaatg agtcctcata
    31501 ctgatattca aactattgca aaagctgttg aagaagcagg tgcagatggg cttgtaatga
    31561 taaactcatt cggaccatgt atggcaatag atgtaaatac aggataccct ataatgggaa
    31621 gtaaagctgg atacggatgg ttatcaggtg ctccaataag acctctagca ataagatgta
    31681 tatacgaagc ttctcaagtt gtaaatatac caataatcgg tgttggtgga atcacttgtg
    31741 gaaaagacgt agctgaaatg atgatggctg gagcaagtgc tgttcaagta tgtacagaag
    31801 caatccttaa aggaccttct atctatggga aaattgctaa agaattaaat gaatttttag
    31861 atgagaatgg atatgctgat gttaatgaaa taagaggatt aactcataag aaaatggcag
    31921 ctagaggatt tagaactcat tcagtagctc cagtagtgga tgataacaag tgcatcaaat
    31981 gtaagcaatg tgtaacaagc tgtgtatacg aagctttaga agttacagat aagttgaaca
    32041 tagatgcaga caagtgtttt ggatgtggat tatgtgtaac tagatgtcca aaaggagctt
    32101 taagcatctg ctacaatcaa gtagaagcta tgaatgagta ataattatat agaattttgg
    32161 taattaactt agagtaatag gtattaggat aataaaatat atcagataaa cacgcattat
    32221 gcattaataa tacagtctaa agttaataat tttatgatat tgatttaact aaatattagt
    32281 ttagctgaat attaatttaa ttaggtattg gtttaatctt aatatagatt tactacgtat
    32341 aagtgaacta actagattac tttaagttaa ttttttcata taaaataaat tgatacatta
    32401 caagtaaggt ggagtctata tgggaattgt tttaaaaggt gggaaagtag taacaagtga
    32461 aaaaacttac tgtgcagatg taagaataga agatgaaaaa atagtagata taggttttga
    32521 tatatcaaaa gaaaatgatg aaatagttga tgttagtgat tcttatgtta taccaggagg
    32581 tatagataca cacacacact ttgacttaga tacaggcgtt actaagacag cagataactt
    32641 tgagacagga acaaaagctg ctatagtagg aggaactact actatattgg attttgctac
    32701 acaagctaaa ggatgttctt tgaaagacgg attagaagaa tggcatgaaa aagctaagga
    32761 taaatcctat tcagattatg gctttcatat ggctataaca gattggaatg actcggtttg
    32821 taatgaaatg gaggatatgg tgaaagaggg agtatcttca tttaaactgt atatggcgta
    32881 taaaggaagc cttcaagttg atgatggtgt catatttgaa gcattgagaa aagcagaaga
    32941 gataggtggg attattggtt tccattgtga aaatggggat ataatatgtg aacttgtgga
    33001 taaagcaaag gcagaaaata atttatctcc aaaatatcat caattaacta gaccagctat
    33061 actggaggca gaagctacgt caagacttat gaagatagct gaagttgcaa actctaaagt
    33121 atatgtagtt cacttaagtt gtaaagaatc cttagaggag gttttaaaag ctaagcaaag
    33181 aggtgtggat gtaatagttg aaacttgtcc tcaatacctt ctattaaatg atgaattata
    33241 tgatttagat ggatttgaaa gtgctaagta tgtaatgtca ccacctttaa gaaataaaga
    33301 aaataatgaa atattatgga atgctatttc agataatcaa atagacacta taggaacaga
    33361 ccattgttct tttaattata agggacagaa ggaacttgga ataaacgatt tcagtaagat
    33421 accaaatggt tctccaggtg tagaacatag gatgagtctt ttatacacat atggagtctg
    33481 taaaaataaa atatctatca acaagatggt agaagtaaca tcaactaatg cagcaaagtt
    33541 gtttggaatg tatcctaata aaggaactat agaaataggc agtgatgcag atatattagt
    33601 cttaaatcca aacaaaaaat ccaaaataga agtgaaaaaa caattacaaa atgtagatta
    33661 tacaccttat gaagggtatg aagtggattg tcaaatagaa catgtttatt taagaggaaa
    33721 agaagtagtt agagatggag aattaatctg taatactcca agtgggagat atataaatag
    33781 gcgtctaaca aagggggcta gagaatggta gaatttatat taaatggaga gaaagttgtt
    33841 gcaaaagaag gcaaaaaact tttagacttc cttagagaag atatgaatat aacttcagtt
    33901 aaaaatggat gttcagaagg tgcttgtgga acatgtatgg ttataataga tggtaaagct
    33961 actaaagcat gtgtatttaa aactgataaa ttagctggga aaaacataac aactatagag
    34021 ggattatcag agagagaaag agatgttttt gcatattctt ttgctagtca aggagctgta
    34081 caatgtggat tttgtattcc aggtatggta attagttcaa aagcacttat agacgttaat
    34141 aataatccta ctgaagatga tataaagaaa gctttaaaag gtaatatatg tagatgtaca
    34201 gggtatgtaa aaataattaa agctgtcgat ttagcagcaa agatttttag agaaaatata
    34261 gatgtgccaa aagtaaactg taaaggtcta gtaggtgaaa atcttcctag agttgatgca
    34321 gttggcaaag cacttggata ttctgaatat gtagatgata tgagaattga aggtatgatt
    34381 tatggttcag cagttagagc taagtatcct agagctttag ttaaaaaaat agatatagaa
    34441 gaagcaaaag cttatccagg agttgttgca gttctaacag ctaaagatat tccaggaact
    34501 ataaaggtag ggcacttaaa gaaggattgg gatactttaa taccagaagg tgaaataact
    34561 agatacttag gagatggaat agttttagta gcagcagaaa ctagagaagc gttagaagag
    34621 gcaaaaaagc tagttaagat agattatgag gaattaacac cattaagtac tacagcagat
    34681 gcacttagag aagatgctcc aaagatacac gagacaggta atatacttgt taaggaacat
    34741 ttagtaagag gaaacgctaa agaaaagata gaaaattcaa aatatgttgt aactaaaaga
    34801 tatactacac ctgctacaga acacgcattt ttagagcctg aatgtgcagt agctggtcct
    34861 tatgaagagg atggagtaat aatctattca acagaccaag gggtgtttgc cactcaacat
    34921 gaatgtgcag atatgttagg gctaccatat gagaaggtaa gagttataaa caaaatggta
    34981 ggaggaggat ttggtggaaa agaagacatg agtgttcaac accatgcagc tttacttgca
    35041 taccatacta agagatttgt aaaagtacag ttaacaagaa aagaaagtat tattgttcat
    35101 ccaaaacgac atgccacaga aatggaattt acgacagcat gtgatgaaaa tggatactta
    35161 acaggtatga aggcaacgat aatatctgac acaggtgcat atgcctctct tggaggacca
    35221 gtactacaaa gactgtgtac tcatgcagca ggaccataca attatcaaga tattgatata
    35281 acaggaacag cagtatatac taataaccct cctggaggag catttagagg atttggagtt
    35341 acacagagtt gttttgcaac tgaatgtaat ctaaatttac ttgctgaaat ggttggaata
    35401 tctccttggg agataagata taaaaatgca ataagacctg gtcaagtttt accaaatggt
    35461 caatttgcag atgaaggaac agcaatggca gagacactag aagctgtaaa agaagagtat
    35521 gaaaagcatc cacgtgcagg tatagcttgt gctatgaaaa atagtggggt tggagttggt
    35581 atacctgatg ttggaagatg tagattggtg ataaaagatg gcaaagttca cataagaaca
    35641 agcgcttctt gtataggtca agggcttggt actgttatga ctcaagtagt ttgtgaaact
    35701 gctagcctac ttcctgaaaa tgtagtgcat gaagttccag atactcattt aacaccagac
    35761 tctggtaata ctacggcttc aagacagaca gtatttacgg gagaagctac taaggttgcg
    35821 tcaaataagt taaaagaagc tttagactct ggcaaaactt tacttgattt agagggagaa
    35881 gagttttatg gagaatatga aagtattaca gataaaatgg gttgtgacaa agaaaatcca
    35941 gtaagtcatg ttgcttatgg atatgcaaca caagttgtaa tgctggatga aaatggaaaa
    36001 ttggaaaaag tagtagcagc acatgatgtt ggaagagcta taaatccaat cagtttagag
    36061 ggtcaaatag aaggtggagt agttatgggt cttggatatg ctctaaccga agattaccca
    36121 gtagttaact ctgttcctac agctaagttt ggtacattag gtttatttaa atctacagat
    36181 ataccagaaa tacaagcttt aatagttgaa aagaacaaag atttattggc ttatggggca
    36241 aaaggtgtag gagaaatttg tacagttcct actgcaccag cagtacaaaa tgcctattat
    36301 gtatttgata aagaatttag aactgaacta ccactacaaa atactccata tagtaaaaaa
    36361 aagtaacaaa aaaaagaaat attaaataaa aaaatagatg aaattgtaaa taaaaaaaac
    36421 ccttttgcat aaggtatatt ttaaatgata gaatatacat atacaggagg gtttttcttt
    36481 tataaaataa gtttaataaa attgagaggt gagcagaatg aattttaata atagtctaga
    36541 attaactcag tctcagaagc taataatgac tacacaactt aagcaatcat tatcaatatt
    36601 aaatatgagt aagttagagt tagaagaaga gataaaaaga gaagcagaga ataacccatt
    36661 agttgaagtc gagaaaagtg gggagataaa ttgggaagag tatataaaag acatggatag
    36721 gtcaagacgt ttagatagaa cagagataag ctataatcct gataatgaag ttaatctaga
    36781 aaatttagtt aaatattcgt caaatttata tgaagattta aaatttcaga taagcctgta
    36841 taaacttaca gacaaagaat tggaagtctg tgagtatata attgatagtc tagatgagga
    36901 tggatattta agaactgagg aaaaagtaat tgttgatacc ttaaagatag atgaagaact
    36961 ttttgaaaaa tgtttaatta gtgttcagca gttggaacct agtggagtag gggcaagaag
    37021 tctttcagaa tgtctgatga tacaaatggc tagtttagga atatataatg aaattttaga
    37081 ggaaatcgtt acaaaagact taaatttaat tgcaaataat aaatacaaag agataagtaa
    37141 aaagtacaat atgtcattac aaaaatgtgt tgaactaata aacataatta agacattaga
    37201 cccaaaacca ggaagaactt gttcagtaga gaaaagtgta tacattcaac ctgatgtaac
    37261 agtagaaaaa attgatgatg aatttattgt atatttaaat gaaaaagatt cttatcaaat
    37321 aagaatcaat agttattata aagacatatt aaaaaattca cagtctgatg aaagtgcaaa
    37381 agaatttata aaagaaagac taaattcagc aacaggactt atgaaaaata tagagagtag
    37441 gaaaactaca gtacttaaga ttgcagaaga gatagtaaaa gctcaagatg aatttttgag
    37501 aaaagggaca aaatatataa aacctttgaa aatgaaagac atagcagaaa aattagaatt
    37561 tcatgaatct actataagta gaggtgtaaa tggtaaatat atgctaacac cttttggcgt
    37621 atatgaattt aggtactttt tcagtagcgc cattgagact gagaataatg aaatggcatc
    37681 tagtacaagc ataaaaaaaa ttataaagga aacaataaaa gacgaaaata aaaagaaacc
    37741 tttaagtgat gaccatatat ctaagttact aaaagaaaaa ggaatcaatg ttgcaagaag
    37801 aacagttgcc aaatatcgtg aagaattagg aatcccatct tcaagtaaga gaaaagaata
    37861 ctaataagta aaagacaaat atcagtattg taaggggaaa aggctaatta aaagatgaat
    37921 gtggattagg ctaaaaattt tatgaaataa aataatagaa tatggaaaag tattaaaatg
    37981 ctataagata ttaaaaaact ggggaacatt aaaacattaa aatagcaaag aacataaaaa
    38041 actaaagaag attaaaacat taaagaacat tagagctttg aggaatatta gaactttaaa
    38101 gaatattaaa atttataaaa tataaaaata ctaaaaagaa ttgaaaaact ggggaacatt
    38161 aaaatattgt aaaatgctaa aaaactagaa aacataatta aataagtgct tataaagaat
    38221 gattaaatta aataagtgtt tgtaaagaat aattagatat tagaaaaaga agaataccta
    38281 gataaataaa ctattattaa atttaataat cacaaaagga aaacacaggg tataaataaa
    38341 aatcttaact agataaaagt aaacttagtt gtagataaaa ataactaaaa ataaaagtaa
    38401 ctagaaataa aattatttat agtaataaat attagaatga aaatataaaa aaatatatat
    38461 aaaaagcctt atataaaaag aaaaaatact gtgatttgaa aatggtggag aaatatttta
    38521 taaaataaaa aaataaaaaa gtttttgaaa aactattgaa atctatacgc aaatcctata
    38581 aaataaaaat atcttgtggg acttaaataa acactaggga cgaaaaaagt cccgaaagga
    38641 aagcaataag gagtatgtta tgaagaactt attagaaatt caaaagaaat tgattccaca
    38701 ggcaattgaa cttatggaaa gaagatactc tattttaaga caaatatctt taagtgaacc
    38761 aataggtagg agaacattat caaatgtatt agagattagt gaaagaataa tcaggtcaga
    38821 aactgagttt ttaaaagaac aaggacttat agacgtagct gtatctggga tgactatcac
    38881 taaagaaggt tctgatttgt tagataaact taaggacatc atgagtgaca tcatgggatt
    38941 gtctaagtta caagatagag tcagagaaaa gcttggaata aaaagggtaa tattagttcc
    39001 tggaagctgt gatgaaaatg gaagtttact aaaagatgta actagatatg gatgcgagta
    39061 tttcttaggt attttaaaag atggagatat cgtttcaata acaggaggaa gcactatgtt
    39121 agagttttca aacgttatta aaactgataa gagataccca aactctacaa tagttccagc
    39181 tagaggaagt atgggaactg atgtagagat gcaatctaat aatatagttg cttcaataag
    39241 taagaaatta cattctaatt gcaaactttt acatatacca gatgagttgg atgaaagtgc
    39301 aatgaaaact cttagccaag tgccagagat taagacaact ttggagtata ttaaaagaac
    39361 agacatttta gtttttagta taggtagagc agatgtcatg gcaaagagaa gaaaacttcc
    39421 agaagacaag gttgatgaga tattatctaa aaatgcagtt ggagaagcat ttggatatta
    39481 tttcaacaaa gatggagaaa tagtttacaa actgaacact gttggaattg atttagaaac
    39541 agttaaacaa gtaaaagaaa ctattgcaat ctttgcaggt actgaaaaag tagaagcctt
    39601 attagctata tcaaatatgc ataaaaacat ggttcttgta acagatgaag aaagtgctta
    39661 taagatattg tcattattat aaaagtcaag acctagcaca gagctagaaa acatacattt
    39721 actttatagg aggtagcgaa aatggttaaa gtagctataa atggatttgg aagaatagga
    39781 agattagcat taagaaaaat gatggagcaa caagataagt tcgaagttgt tgcaataaac
    39841 gacttaacag acgctaaaat gttagctcat ttatttaaat atgatacagc tcaaggaaga
    39901 tttaacggag aaatagaagt taaagaaggt gcttttgtag taaacggaaa agaaataaaa
    39961 gtaacagcag aaagaaatcc tgctgaccta ccatgggctg aattaggagt agatatagta
    40021 ttagaatgta caggattctt tacatctaaa gataaagctg aagctcatat acaagcaggg
    40081 gctaagaaag ttgttatatc tgcaccagct actggagatt taaaaacaat agtattcaac
    40141 acaaacagtg atatattaga tggttctgaa acagttatat caggagcttc ttgtactact
    40201 aactgtttag ctccaatggc taaagtatta aatgataaat acggtataga aaaaggatta
    40261 atgactacta tacatgcata cactaatgac caaaatactt tagatggtcc tcatccaaaa
    40321 ggtgatttaa gaagagctag agctgctgct ggaaacatag ttccaaacac tacaggagct
    40381 gcaaaagcaa taggcttagt aataccttca ttaaaaggaa aattagatgg ggcagcacaa
    40441 agagtaccag ttgtaactgg ttcaataact gaattagttt gtacattagg aaaaaatgtt
    40501 acagttgaag aaataaatgc agctatgaaa gaagcttcaa acgaatcttt cggatatact
    40561 gaagaaatgt tagtatcttc tgacataatc ggaataagct acggttcatt atttgacgct
    40621 actcaaacaa aagttatgga agtagatgga aaacaattag ttaaagttgt ttcttggtat
    40681 gataatgaaa tgtcttatac ttctcaatta ataagaactt taggatactt tgcacaatta
    40741 gcaaaatagt cagttttgag aaaatagaaa agtagattga caaagtccga gtttgtagtt
    40801 taatactacg gactcaggac tttttttaag aaaaaatata taaaatacac ctaaataatg
    40861 ttacatacta tatataaaaa tatgataata tgatatattt aatagggtaa aagtatgtac
    40921 tatattatac ttggcaataa aaaattactt tattaaaagg agagatattt atgtcaatgc
    40981 ttaacaaaaa aactatagaa gatattgatg tgtgcggaaa aaaagtatta gtaagatgtg
    41041 actttaatgt gccattacaa gatggtgtaa ttactgatga aaatagattg aacggagctt
    41101 taccaactat tcaatattta atttctaaag gtgcaaaagt tatattatgc tctcacttag
    41161 gaaagccaaa aggagaagct aagccagaat tatctttagc accagtagca aagagattat
    41221 ctgagatgtt aggaaaagaa gttgtatttg cagcagacga taatgttgta ggagaaaatg
    41281 caaagaaagc aactgaaaaa atggaaaacg gagatgtagt attattagaa aatactagat
    41341 atagaaaaga agaaactaaa aacgaagaga atttctctaa agaattagca tcacttgctg
    41401 aaatatttgt aaatgatgcg tttggaactg ctcatagagc acattgctca acagttggtg
    41461 caggtgagtt cttacaagag agagtttgtg gatacttaat tcaaaaagaa ttaaaattct
    41521 taggagaagc tgttgcaaac cctgtaagac catttacagc tatacttgga ggagctaaag
    41581 tttctgataa attagctgtt ataaacgaat tattagaaaa agttgataat ctaataatag
    41641 gtggaggaat ggcttataca ttcttaaaag ctcagggtta tgaagtagga acttctttac
    41701 ttgaaataga taaagttgaa tatgctaaag aaatgatgga aaaagctaag aataagggtg
    41761 ttaacttatt attaccagta gacgttgtta tggcagacca ctttgcacca gatgctactc
    41821 caatagtaac aaaagatgct aatgtaaaag aagattacat gggactagat atgggaccta
    41881 agactatagc aaactttgta aaaactataa aagaatcaaa aactgtagta tggaatggac
    41941 caatgggagt atttgaattt gaaaactttg caaatggtac attgtcagta gctagagcta
    42001 tggcagagtt aactgatgca actactgtaa ttggtggtgg agatagtgca gcagcagtta
    42061 atcaattagg atttggtgat aagatgactc acgtatcaac aggtggagga gcatcactag
    42121 aatttttaga aggtaaagaa ttaccaggta ttgcagcatt agataataaa taaactaata
    42181 tatagaggag attgtaaaat gagaaaacct ataattgcag gaaactggaa aatgcataaa
    42241 acaataaaag aagctttaga atttgtaaat gaagttaaat ataaagtaaa ttctgataaa
    42301 gtagaagctg ttatatgtgc accatttact ttattaaagg atttaaaaga agctactaag
    42361 ggtacaaata taaagatagg tgctcaaaat atgcactttg aagaaaaagg agcatttaca
    42421 ggagaagttt cacctcttat gttaaaagaa atagatatgg actatgttgt aataggacat
    42481 tcagaaagaa gacaatactt caatgaaact gatgaaactg taaacaagaa agttttaaaa
    42541 gcattagaag taggaataga cccaatatta tgtgttggag aaactttaga gcaaagagaa
    42601 gctggaaaaa ctaaagacgt ttgtaaaatt caagttgaaa aagctttaga aaatgttctt
    42661 aaggatgatt tagcaaaagt agttgttgca tatgagccaa tctgggctat tggaactggt
    42721 aaaactgcaa ctgctgaaga tgctaatgac gttatatctt atataagaga agttataaaa
    42781 ggattatatg gagaattagc taatgaagtt agaatacaat acggtggaag tgttaaacct
    42841 tcaaatgtag ccgaaataat gggtcaaagt gatatagatg gagctttagt aggtggagct
    42901 agtttagcta gtaatgatta cctagacctt gttaatttct aaggagtgaa atgatgaaaa
    42961 aaccagttgc tttaattatt atggacgggt ttggatacaa taaggatgtt aagggaaatg
    43021 caattgctga gtccaaaact cctaatttag atagaataaa aaaagagtat ccaaacacat
    43081 tgataaatgc tagtggtctt gatgttggtc tacctgatgg tcaaatggga aattcagaag
    43141 ttggacacac taacataggt gcaggtagaa tagtatatca agatttaact agaataacaa
    43201 aatctataaa agatggtgac ttttttacta ataaagtttt atgtgaagca atggataatg
    43261 caaaagaaaa ttctcttcat gtaatgggac ttttgtctga tggaggagta cattcacata
    43321 tagaccatct taaggctata atcaaaatgt caaaagataa aggcgtacaa aaggtgtatg
    43381 ttcacgcatt tactgatggt agagatactg accctcaaag tgcattagaa tatgccaaag
    43441 aagtacaagc tagtatggat gaaattggag taggtgaatt tgctacagtt tctggtagat
    43501 actacgctat ggatagagat aagagatggg aaagagtaga gcttgcatac aatgctatgg
    43561 ttagaggtat tggtgaaaaa gcaaattcta ttgaagaagc tattcaaaac tcttatgatg
    43621 atggaaaaaa tgatgagttt ataatgccaa cagttattat gaaagatgat aaaccagttg
    43681 gaagtataaa ggaaaatgat tcaattatat tcttcaactt tagaccagat agagctagac
    43741 aaataactag agctttagtt tgtgaagagt ttgatggatt taaaagagaa gatattaaaa
    43801 gcttctttgt atgcttaaca gagtacgata taactataga gaatgtgcat atagcatttg
    43861 gacctcaatc tttagccaac actctaggtg agtatctagc taaaaatgga aaaactcaac
    43921 ttagagctgc tgaaactgaa aagtatgcac atgtaacttt cttcttcaat ggtggagtag
    43981 aagagccaaa taaaggtgaa gaaagactat tgataccttc tccaaaggtt gcaacttatg
    44041 atttaaaacc agaaatgtca gcatatgaat tgacagataa agctttagag aaattaggag
    44101 aagataagtt tgactttata gtgttaaact ttgcaaatcc tgatatggta ggtcacacag
    44161 gaagtataga agcggctgta aaggctgttg aaactgtaga cacttgtgta ggaaaattgg
    44221 tagataaaat agtagagtta ggtggaagtg caataataac tgctgaccac ggaaatgctg
    44281 aatatatgtt agacccagaa acaggaaaaa ctgtaacagc tcactctatt aatccagtac
    44341 catttattgt agttggtcaa gaatatgaaa gtgcaaaatt attggatgga ggaagattat
    44401 ctgatatagc accgactgtt ttagatatga tgaagttaga aaagccagaa gaaatgactg
    44461 gtcattcact tatttcaaaa tagtcgattc tgataagtag agaataatat ataaactaca
    44521 atataaattt aaaaataaca cagtttaggt ttattttgta aggtttagac tttatgacat
    44581 aagatttaga ttttgtaagg tctggatatt gtaaggtgta gatgaaagta tattttataa
    44641 aatttacaag atagacaatt tataaaataa aaaattataa tatagataat aatgtaaata
    44701 atgatataaa taataacaat
//
LOCUS       DAEMAE010000009        42220 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEMAE010000009 DAEMAE010000000
VERSION     DAEMAE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.009"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..852
                     /locus_tag="KPH93_000419"
     CDS             58..852
                     /locus_tag="KPH93_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG1687256.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            876..1319
                     /locus_tag="KPH93_000420"
     CDS             876..1319
                     /locus_tag="KPH93_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG1687257.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(1408..2694)
                     /locus_tag="KPH93_000421"
     CDS             complement(1408..2694)
                     /locus_tag="KPH93_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG1687258.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEEFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHINDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2912..3802)
                     /locus_tag="KPH93_000422"
     CDS             complement(2912..3802)
                     /locus_tag="KPH93_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG1687259.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWFIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            4094..4438
                     /locus_tag="KPH93_000423"
     CDS             4094..4438
                     /locus_tag="KPH93_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG1687260.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(4597..6693)
                     /locus_tag="KPH93_000424"
     CDS             complement(4597..6693)
                     /locus_tag="KPH93_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG1687261.1"
                     /translation="MIKFKIYISGLYSGNVIFGDDLLIEKLNPFTNKIESLKPISREE
                     NTYYLNLTKIDLKSLFKNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWDKTHKDEEIYSIRFGGNYMV
                     DMKDGIKLSTDVYLPNFIDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSDGTVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNGMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDRSMYLISDGNANTSLGNGRLV
                     FDSDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNSYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLVLPIENK"
     gene            complement(6707..7867)
                     /locus_tag="KPH93_000425"
     CDS             complement(6707..7867)
                     /locus_tag="KPH93_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG1687262.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFIFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(7886..8953)
                     /locus_tag="KPH93_000426"
     CDS             complement(7886..8953)
                     /locus_tag="KPH93_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG1687263.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHAIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDNKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIRKAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            9305..9871
                     /locus_tag="KPH93_000427"
     CDS             9305..9871
                     /locus_tag="KPH93_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687264.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKSNVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(10090..11076)
                     /locus_tag="KPH93_000428"
     CDS             complement(10090..11076)
                     /locus_tag="KPH93_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG1687265.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSKGEVEKFLSNEYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLSDIAV"
     gene            complement(11106..11801)
                     /gene="pxpB"
                     /locus_tag="KPH93_000429"
     CDS             complement(11106..11801)
                     /gene="pxpB"
                     /locus_tag="KPH93_000429"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG1687266.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAVEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(11821..13041)
                     /locus_tag="KPH93_000430"
     CDS             complement(11821..13041)
                     /locus_tag="KPH93_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG1687267.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVRPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(13070..13861)
                     /locus_tag="KPH93_000431"
     CDS             complement(13070..13861)
                     /locus_tag="KPH93_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG1687268.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKDIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            14449..15078
                     /locus_tag="KPH93_000432"
     CDS             14449..15078
                     /locus_tag="KPH93_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1687269.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAKTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(15191..16705)
                     /gene="glpK"
                     /locus_tag="KPH93_000433"
     CDS             complement(15191..16705)
                     /gene="glpK"
                     /locus_tag="KPH93_000433"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG1687270.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            16948..17490
                     /locus_tag="KPH93_000434"
     CDS             16948..17490
                     /locus_tag="KPH93_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1687271.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKIFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            17504..19996
                     /locus_tag="KPH93_000435"
     CDS             17504..19996
                     /locus_tag="KPH93_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1687272.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGESKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKKELSKKLRSMESQKKELQSKLNNSINKKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNLGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            20417..20815
                     /locus_tag="KPH93_000436"
     CDS             20417..20815
                     /locus_tag="KPH93_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687273.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(21032..21241)
                     /locus_tag="KPH93_000437"
     CDS             complement(21032..21241)
                     /locus_tag="KPH93_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687274.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(21273..21482)
                     /locus_tag="KPH93_000438"
     CDS             complement(21273..21482)
                     /locus_tag="KPH93_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687275.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            21781..22200
                     /locus_tag="KPH93_000439"
     CDS             21781..22200
                     /locus_tag="KPH93_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687276.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLRKTEYNKNKDEKIDK"
     gene            22428..22883
                     /locus_tag="KPH93_000440"
     CDS             22428..22883
                     /locus_tag="KPH93_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687277.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(23107..23367)
                     /locus_tag="KPH93_000441"
     CDS             complement(23107..23367)
                     /locus_tag="KPH93_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687278.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(23630..23983)
                     /locus_tag="KPH93_000442"
     CDS             complement(23630..23983)
                     /locus_tag="KPH93_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687279.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(24000..29231)
                     /locus_tag="KPH93_000443"
     CDS             complement(24000..29231)
                     /locus_tag="KPH93_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBG1687280.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSKVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDNNMFIVMNDGSLYACGLNNAGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGSSTFVLKNNGTLYSCGLNSSGILGLKDNTNRNIFTKI
                     EIENIKEFCVESNYIVALNHSKELYGWGNQSYIVYGDNRNYPYKDTRVSNVEKIATWS
                     DTLYILDSTGAAKTIGYSYNGSGGYPAPSTSSSYQSKGYNAWNTSYRTLEFYNTAQTK
                     LINLFAFYRGCMFFDESDRAYCIGENNMKFTSSSQITPESELRFSSNSGIYHTNSDGG
                     VYTCYQWTYKLIRCSVFDSSKSVVGNSKNILSLLKNNSTFRCTGSCLTYGQTNQNWSS
                     YLSDNCNGAVSLGNEFILKNYSGESVLKGYGKSNNGEFGSSTSISNASNYDTGLKDIK
                     DIIVKNNTVVVVDKNNNIYVTGTNQFNKLGIGEYNNQPIKKFTNITEQSNSFIFMDDI
                     KEITTSRNTMFIVKNDGTAYATGNNSSGQLGLGDTINRNKFTQINIDNIKKISTSIDG
                     NTTFAIRNDGTLYSTGLNTKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDD
                     NTLYSCGENTHGQLGLGSESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCC
                     GYNNNSQLGMGNTTDQYSFIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGL
                     NSNSNQSSFSEIPISNVVKVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIK
                     KIASKAKDIGMNYRCGHYVSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKIL
                     PLEYANIAIKDTNDIYICGLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIK
                     NRHNFILLNNKIVIPTTKDIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLN
                     DGTMYGCGTNYHGELLQDLSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIG
                     KNSEYQLGIGHNNPVTELQRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALG
                     LGSDSENTIIKFFTKALTDIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEF
                     LKEFTIIPISEDVNTVKDVLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNK
                     DISRIEMLINGESVKSVSDLITEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFI
                     FKESTGNSIIKDSYVMIGNRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINK
                     TKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIK
                     RPSMIFRYDAENNEPQA"
     gene            complement(29253..30239)
                     /locus_tag="KPH93_000444"
     CDS             complement(29253..30239)
                     /locus_tag="KPH93_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG1687281.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVNIGDVNILTTYSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLNKYSYEIR"
     gene            complement(30240..30938)
                     /locus_tag="KPH93_000445"
     CDS             complement(30240..30938)
                     /locus_tag="KPH93_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG1687282.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(30935..31987)
                     /locus_tag="KPH93_000446"
     CDS             complement(30935..31987)
                     /locus_tag="KPH93_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG1687283.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSLVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIARCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(31980..32408)
                     /locus_tag="KPH93_000447"
     CDS             complement(31980..32408)
                     /locus_tag="KPH93_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG1687284.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(32408..32734)
                     /locus_tag="KPH93_000448"
     CDS             complement(32408..32734)
                     /locus_tag="KPH93_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG1687285.1"
                     /translation="MANPINEFIGIIREEGKHHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMLDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(32750..34282)
                     /locus_tag="KPH93_000449"
     CDS             complement(32750..34282)
                     /locus_tag="KPH93_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1687286.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYVGGTEFSAIFTA
                     YYPGPGIEGGDTDCREKKLNPSKKTCAAPMVGAYEKSYYTKEFLSKHPLFKYGDEVSI
                     VTGVSGRDGVYKVNDNGSAIIIEKDGTYHIDVLVKNAEEMKRFGKRKGKIIIGGYSGN
                     ASNKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKG
                     KKVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQTGDVVKISKLSSRRDFNTARR
                     VL"
     gene            complement(34564..34986)
                     /locus_tag="KPH93_000450"
     CDS             complement(34564..34986)
                     /locus_tag="KPH93_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687287.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(34986..37385)
                     /locus_tag="KPH93_000451"
     CDS             complement(34986..37385)
                     /locus_tag="KPH93_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687288.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEIEKTKNKLLGNLKKIGGK
                     VWKIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQREVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSERMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWETVWATIGPLLEDAWIIVEPVLGGLFNLFNDICNII
                     KDVCKWWQNLVEKINKNPIIETVRSIIDDWNAGTSKSGSNAFGLNYVPYNDYPTRLHE
                     GEMVLTKQEANQYRSGKNGGNISIAKLADTIVIREEADIEKVTSKLVSSIKMAQLGGV
                     L"
     gene            complement(37385..37552)
                     /locus_tag="KPH93_000452"
     CDS             complement(37385..37552)
                     /locus_tag="KPH93_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687289.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGMCPLTGGGM"
     gene            complement(37579..38025)
                     /locus_tag="KPH93_000453"
     CDS             complement(37579..38025)
                     /locus_tag="KPH93_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1687290.1"
                     /translation="MSENGLSENINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(38139..38567)
                     /locus_tag="KPH93_000454"
     CDS             complement(38139..38567)
                     /locus_tag="KPH93_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG1687291.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(38581..39645)
                     /locus_tag="KPH93_000455"
     CDS             complement(38581..39645)
                     /locus_tag="KPH93_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG1687292.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGDSDIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLIDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(39650..40093)
                     /locus_tag="KPH93_000456"
     CDS             complement(39650..40093)
                     /locus_tag="KPH93_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687293.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSNSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYALNFSVTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(40342..40782)
                     /locus_tag="KPH93_000457"
     CDS             complement(40342..40782)
                     /locus_tag="KPH93_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687294.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(40887..41084)
                     /locus_tag="KPH93_000458"
     CDS             complement(40887..41084)
                     /locus_tag="KPH93_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687295.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            41253..41573
                     /locus_tag="KPH93_000459"
     CDS             41253..41573
                     /locus_tag="KPH93_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1687296.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            41655..42161
                     /locus_tag="KPH93_000460"
     CDS             41655..42161
                     /locus_tag="KPH93_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687297.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT        13815 a         7281 c         4749 g        16375 t
ORIGIN      
        1 atgtattttc tgttatctgt tattatcatc aataataaac ataagggggt atatattatg
       61 aaattgtatt taagtgctga tatagaaggt acttgtggta ttgtaaattg ggatgaaaca
      121 aaacttgaat gtgaattttc acaacactat agaacacaaa tgagtaaaga gataacagca
      181 gcatgtgatg gagctactca atctggtgta catgaaatac ttgttaaaga tgcacatgac
      241 agtggtagaa atattaatcc aaatatttta cctgaaaacg tcaaaatact tagaggatgg
      301 accaaagacc cactagttat gatggctgga attgatgaaa gctttgatgc atgtatattc
      361 actggctatc actctggagc tacttgtaat ggtaatccac tttcccatac tatggatatt
      421 gattatgact attttaaaat aaatgatgaa atagcatctg aatttactat aaatgcttat
      481 attgctgcat attataatgt tcctgtagca tttttaagtg gagatgaaat gctttgtgag
      541 agtgcaaaaa aactaaatcc aaatatagtc actgttcctg tgtcaaaagg aattggaaat
      601 ggttcaattt caatccatcc aaaccttgca ctaaaaaaaa ttgaaaaagg tgtcaaaaaa
      661 gctttatctg gagatttatc taggcactta ataaaattgc cagataaatt taagatagaa
      721 ataaaattta gagaacatta taaagctttt aaggcctctt tttatccaaa tatgaaaaaa
      781 atagattctc aaacagtact atttgaaaca gaagactatt atgaattttt aagaatgtta
      841 ttatttatat aaatttcata tcataaatta aggagttgat taagataagt aataaaagaa
      901 ggattccttt aggcgaattt atttctgtat ttttattttt tgctgttttt tttggtataa
      961 ctttttgtat tttcacggtt ataggaattg gcttcttatc attcttagga tttgaatata
     1021 aatctcttgg agctgtatta attttctttt taatatattt ttgtataacc actccaattg
     1081 attttctatg cactactatt ttagatatat tcagatatgt aaacaaatta ccatactcaa
     1141 tatataaact ttgcgaattt ataattgatt ttatactaac ttttctagct atgaacataa
     1201 ttgatacctt tatggattca gtaacgattc ctttaagtac agaaatctta ttcgctttac
     1261 tttcttatct tttatcagaa tgtatggatt tttttgatag ggataagaaa aaaccttaat
     1321 aaaaaagata gattccttaa tagaacacat attttcgtat ttctattatt taatctatct
     1381 ttattttatt taataaattt ttattaatta aaatatagtt tcttctatct ccttagtcat
     1441 ttgcttagca ctatcaaaaa attcttctat atacactact tcatcactga ccctagaata
     1501 ttctgctgca aatgccatta catgactttg tatagcttct tttgctgcta ctctaccctt
     1561 ttctagttct ttatttgaaa ctaatttaat aaattcagct ataagatttc tatcatattc
     1621 ctctaccata ttctgttgat ttgatggatt cataactatt tcttcgtttt ttccaaattc
     1681 tttaattctt atctcatttt tatttaaatc cccaccaact tcaccatggc taaacattaa
     1741 ctttatagtt cttccatttt ctttagtaaa tgcacataaa ttaaaagtag ctgtcactcc
     1801 attttcaaac tgtagtatat ttaccatatt gtctacaaca ttattgtcac atctataaac
     1861 acatcttcca tatggacttg tcttaagtat atttaataga ttctcttcag ttggatttat
     1921 gtgaacagct ctatttatac ttctatcttt ttctaaataa attctctttg ctgaatatgg
     1981 acattttttt tcaactaaac actgaagaca attttgtgcc atctctccac tagcattttg
     2041 gttattcaga tgctttaagc ttccaaatgc agatattttt ttgcatctac ttcctactaa
     2101 atataataaa atatccatat catgacaact ctttgctaaa attagaggac ttgtttcatt
     2161 gctatttctc caattccctc tagtaaagct atgtgcaaaa tgccaatatc ctatattttc
     2221 attatattgt atactaacta gctctcctaa ttttttgctt tctacaattt cttttaattt
     2281 attataaaat ggcgaatatc tcagtacatg acaaatcata aaaatcttat ctttatactt
     2341 atcacacaag tcatttatat gtaccaaacc atctaaacta ttagacattg gcttttctaa
     2401 tagtacatga taaccttttt caagagctag ttttgctaca tcataatgtc tatcatcatt
     2461 tgtagctatt attactgcat ctgccatctt gtcatgttta aaaaattctt ctaaagtttc
     2521 aaatacattt ttactatcta ggttatattt ttgtgtaaat agttctcgtc ttcctttttt
     2581 aggttctgct acagcaacta tttcacatac atcagaattt tcataaatat atggagcata
     2641 tgcatatgtt cctctattac ctgctccaat cacagcaact ttcacttttt ccatacttta
     2701 atttatcccc tttatattat ttatactact ctattatagt ctatttttac ggacatttgg
     2761 atatttttaa taaaagaaac agcagttata atttttcaat tttaactgct gttataagtt
     2821 aatataccaa acatttttaa ttaaattttc tttaatttta atacaaagac ttaaatattt
     2881 aatacaaact ataaaaacac ttttatctag cttacttctt cttttctttg tctaatttat
     2941 ctaatttctt attatataaa aacatataaa atgctgccga aaatactatt aaatatccaa
     3001 caacacttaa catatctgga tagacattga ataagaatat acttaaaatt gctgaaaaga
     3061 ttatattact atagtcaaat atagagattt ccttagcggg tgcatattta tatgctaaag
     3121 ttattccaaa ttgacctaga cttgcaaata ctccacctag taatagatat ataagttgca
     3181 taagagtcat tggttcatat actgcaaaag caattggtcc tacagtaact aatgaaaaaa
     3241 ctgaaaagaa aaatacaatt gtatagtaat cttctctatc tccaagaact cttaaacaag
     3301 tatatgccat tgctgcaaat acagctcctg caattcctcc aagatatgga attacttcta
     3361 cactaaatgt tggttttatt atgaataaag ctcctataaa tgcaattata atagctccta
     3421 tctgttttac atttattttt tcttttagag caattgcaca aaatattact acaaggaatg
     3481 gactcatttt atttagcata ttagcatctg aaagcatcaa cttgtcaatt gcatagtagt
     3541 taaatataac tcctagagtt ccaaatattg acctatataa aagtactttt aaattttctt
     3601 ttttgccagt tagacttcct ttatgtttta tgataaacca aaaagctatt atagctgata
     3661 ccagatttct aaagaacact ttttgaaaac ttggtaaatc tccagctaac tttacgaatg
     3721 cagacatcat tgcaaagcca agcgctgatg ctattataaa cattatacct tttattcgat
     3781 ttgatttgat tgtgctactc atacgtacct cttttctata taaatgtata aagtcatatt
     3841 aattaaaaac ttagcttata catcaaataa taattatcta aatattccaa tttatactat
     3901 aattaaaagt gtaaactatg tcaagtaact aataaatctt ttcaattaat aacaataata
     3961 attatagtta aaaagttata tgtttttatg gattattatc agaatatata atataatttt
     4021 atatagtata gacttttttt aatttattat ttagattttt aatagatttt ttaactatgg
     4081 aggtatttaa aatatggaaa taaagagata tgaaggaact ggaagaatga gtagagctgt
     4141 agttcataac aatacagttt atttatgtgg acaaactcat gctgaaggag gagtaattga
     4201 acaaacaact gaagttttgg ctaaaataga agatttatta aacaaatatg gttcagataa
     4261 gcaacattta ctatctgtta ctatttattt aagagatatg aaagattttg aagctatgaa
     4321 ctctgtttgg gatgcttggg ttgaaaaagg atttgaacct gctagagctt gtgttgaagc
     4381 tagacttgct agagagcatt tgttagttga aatgagcgtt gttgcagcta caaaataatc
     4441 aatttagctt ataggaggct atctcaaaat agagagataa gcctcttttt cattgttgaa
     4501 atgatgaaaa tatatttaat ttcatataaa agaaagtgcc tcacgaacta aaaaattcat
     4561 tttgagacac ctcctcacta tttaatctat ataaatttac ttattctcta ttggcaatac
     4621 caatttagat ggatatttac ctccatggta aatcgtattc tttgcttcta tatattcaat
     4681 actattatat ccctctgtcg tgttgctatt ttggaaaata aagtttttag cactagatgt
     4741 tatatctagt ctaattttat gaccaacttt aaacgtgttt gatatttttg aagttataat
     4801 cgtaaactta taaatctctc cttcttccat aaactctgac ttataaaagc tatttctaaa
     4861 tctagcacta agtataccat cagctaattt aattgaattt ccatctaaat ccacgtccat
     4921 aatttttata acccagtcag tatctttggc ggaacttgat gcaaaaaact ctaactttat
     4981 atcacctgtt acagtaaatt cttcactaaa agcatctgat gtataacaca acatgtcact
     5041 tcttttttct aaatctttgt agctatttgg aactccaact tcattctctg acatatcaat
     5101 tagttgtact gatgggtctt ttggattata tatataacta tcatactttt cttctaaatc
     5161 actatcaaat actaatctac catttcctaa agatgtgtta gcatttccat cacttattag
     5221 atacatactt ctatctatct tatttattat tggccaattt tcttctgtct tccacttatt
     5281 agaacctaca gagtaatact ctactggagc agtcgtatct atgttatttt ctataccttt
     5341 taagtattta tcaaaccata atagataatt ataatccaaa tcatatctta atgaactatt
     5401 tcctaaagaa ataccattta tatctcttaa agtatttgaa ttatgcatcc atggacctag
     5461 aatagccttt ctctttcctt tttcatagtc ctttataaca tctaaggcct ctgtagttcc
     5521 cataccatta tcatcatacc acccagatac aaccaacgca ggtacattta ttttatcttt
     5581 ctgtaaatgc caatccatca aatcccaata ctcatctttt tctactctct ttaaccattc
     5641 ttctagaaat tcaattctat atccaagagc ttcaacaggt atattctcta ttggtcgtat
     5701 gtttaaaaca tcatcccaat catctcttac cattttactt ctatcaactt tatttcttga
     5761 taccataaac gcccatgcaa gcataccaga tacgaaagct cctccttttc ttggtatatc
     5821 aataaaaggg cttcctgatg ttactatact aactaaagct ttaagatgct tattacctga
     5881 agatgcagct gcccactgaa catagccaag atatgatgct ccaagcattc caactgttcc
     5941 atctgaccat tcctgtgata ctatccagtt tatagtacta tctccatctt ctctttcatg
     6001 aatcataggt tcccacttac cttctgattc gtttcttcct ctaacatcct gtattacaac
     6061 agcatatcct ctttgtacat atttataata tatttcttta tcattttctt ttccatatgg
     6121 agttctcata agaattgttg gtgctttttt ggtagaatct ataaaattag ggagatatac
     6181 atctgtagaa agttttatac catctttcat atcaaccata taatttccac caaatcttat
     6241 agaatatatc tcttcatctt tatgagtttt atcccattct tttaatacag tatactcttc
     6301 atatccatcc ataattaata tagtacaagt ttctctggat aaacaaataa aacctactat
     6361 tttattatct acaataatta tatctagtgg aaacttttta tttctttgaa cccatatata
     6421 ttcattaagt ttagaatgcg tttctcctaa gttatttata actgtgtctt tatctgtatt
     6481 tactaaactt ttagtatata catcaaagtt cttaaataaa gactttaaat ctatttttgt
     6541 aagatttaaa tagtatgtat tttcttctct gcttattggt tttaaacttt ctattttatt
     6601 tgtaaaagga ttcaactttt caattaataa atcgtcacca aagattacat ttccactata
     6661 taatccagat atatatattt taaactttat caattaaact ccccctctat aatcccagag
     6721 acatctttat tgcatatgcc actgatatac ctgcataatt tcctaatact ccaccaatta
     6781 ctcccattac gactgctaca cttacaagtg aattccactt tgcagaagca gctactaaag
     6841 ctgaagtagg accatcagca attgctccta ctatagaaag tattacatat tgatacttta
     6901 tctttaaaag tctagttatg cctaaatgga aaataaatga agcaaaaatt atgctaaaac
     6961 agtaaagtgt tatggtaaaa gttgaaccta aaaactcttt tatatctact gaaaaaccta
     7021 tagtacataa aaagaacaat gcaacaaaca atcctaaatc taaatttccc tttagtttct
     7081 ttactggctt taattgagca attataatcg aaatagtagt aactaaaagt attcttgctg
     7141 cactactaaa actcgaatta aaatatccag caagtattgt tgcaacctct gtaacaacaa
     7201 aacctatagc caacatccaa gctatatctt taatactcca ctcttttgac tccataaatt
     7261 cttcattttg acaatcctct aattctgatt cttctacatg atatggccaa agctttttaa
     7321 acttctttga gttttttagt atagctggta gagcaaacat aaatatcaat gttggtactc
     7381 caattacata gtctgctgca tttgctgcag caagagtttg tctacttaca ttaaggcctg
     7441 ttccaatagc tgtaagatta gcacttccac cagtgtatgt acccacaaac atcccagcaa
     7501 ctttccaacc ttctgatatt ttttctgcaa atactagtcc aaataaaaat gccatgatac
     7561 ttacagttaa tactgctaca aaaattgcaa tcaaaggttc tcttgataat tttgtcattt
     7621 cttttaaatc aacactcatc aacattatag tcatagaaac tggaatagca tatgtagaaa
     7681 tagttccata taactcagta cttactggaa ctacttttaa gtttgaaagt attattccca
     7741 ttatgattat tgtaagtgct ggtcctaaac ttttaatggc tttgtatctt tgtaagtaaa
     7801 aacccaatgc aatcattgat gtcattacag caagtatact acctggattt gaaaaaagtg
     7861 tattcatatt taacctccaa atttatcata agtttacttc tattcctaaa cctggtttat
     7921 tgacaagatt cataatatct ccatccatta caaatcctcc agatatacct tctaattctt
     7981 cacaaaacat aaaactatca aggtcagctt ctgttatgtt ctttttagat gctattaaat
     8041 ttgctgctgc tgttatacca attctagttt ccagcataca tcctaacata caatttactc
     8101 cacttgcctc agctatgttg ttgattttta tagcaggata tattccactt gatttcatca
     8161 gcttaatatt aaatgtatca actgcattct tttttatagc cttaattgca tctattggtg
     8221 aatgtaatga ttcatctatc ataatttttg tattactttt atttcttatg taagcagtac
     8281 catctaaatc ccaatgtgga agtgcttgtt caattgcatc aacattaaat tcttctaact
     8341 ttttgatagt atttatactg ctatttacat tccatccttg atttgcatca attctaattc
     8401 ttatactctc tccaacagct tttctaatta actttacagc ttcaatatcg tcttcaggat
     8461 ttatacctgc tttaagcttc aatatcctaa aaccttcttt tactctttct aaagcttcta
     8521 ctgccatttt ttctggctta tctatactta tagtaatatc tgtttgaact ttattatcaa
     8581 atcccccaag aaccttataa agaggtgcat tcataatttt tcctttaata tcatataaag
     8641 ctatgtcaat agctgctttt gcagaagtat tacctataat aattccatcc atgattgcat
     8701 gtatggtttc aatatctagt gggtctttgc ctattaactc ttttttaaac ataagcaata
     8761 ctgaaagaac attatccaaa acttcacctg taactgctgc aagaggtgct gcttcgccaa
     8821 atccacaata cccttcatca gtttcaattt ttaaaattat actttctcca tattcaatta
     8881 ctccaaatga aactactaca ggttttttta atttaattct aagcttttca aatttgatat
     8941 ctgttatctt catattaccc tccttatttt ttgaagttat aattcaattt ttaaaatttt
     9001 atactttaaa ttatattatt acttcaatat atatgtcaag ataatttact gaaaatttac
     9061 tcttttccaa aatataatgt gataatctta attcaattaa tactcttcta ttaaattttt
     9121 ttattattat tttctttata ttaatttaac aataattcat tttcatagac ttttatttgc
     9181 atttaattaa attttaattg tcaaaatcta ctatgtattc ttattgccac tacttctatg
     9241 ttgtgctata attactttgt tttaagcgaa tattttatat taattttatt atttggaggt
     9301 tcttatgcta ggtgaaaaga tgagaaatat aagaaaaagc aagaaaaaaa cattaagtga
     9361 tgtttccaaa ttgacagatt tatctataag ttatatctct caaatagaaa gagatgctat
     9421 tgaaccatcc ttgtcttcat tgagaaaaat cgctgaagtt ttagacactc ctttatatat
     9481 gtttatggat gacaacaata ctgatgattt agtaattaga aaagaagata gagttatgat
     9541 gaaatttcca aagagtgaaa tgttttacga gatagtatct ccaatgccta caactgaatt
     9601 tacaccttca attttatttg ttgagtttga acttaaacca gaaagtgagg atgctaaaga
     9661 ttatatatat catgcatcag aagaaattat ggtggtaacg agtggtgtgg ttgatatttg
     9721 tatgggcgaa aaggtagtta gattaaatcc aggtgactca acatttataa aatctaatgt
     9781 accacataaa atcatcaatc caagtaaaga tacaatagct cgtggatatg gagttatatc
     9841 acctccaatt tggccaataa aatctaaata aactcaatat aacataccta ttttttgtag
     9901 ataatacaat aaatttaaat atcactttta ttttaaaatt gaagtattta tttaaattta
     9961 aaatatttac tcaaactaaa aaaatagcta tttgactaat ctaaaccatc ttaattatgg
    10021 taaatagtaa atagctattt gtaagttata ttataaatta taataaaaat tgtatttgtt
    10081 aaaattaaat taaactgcta tatcacttaa tacttttgcc tttacaactc ttatattttt
    10141 catactcttg tagatatctt gaatcttatc ttcttgctct ctcaatagtc tatgtgcttc
    10201 atatatatct acaagttcaa attttactac gtcatttggc tttgcttgtg ctaacttata
    10261 caaatctaca cttataacgt tccctatctt agtatagcca ccactagtct gtctatctga
    10321 taacattata ataggttttc catgaccagg tacttgtata gctccataag aaataccatc
    10381 agatataata tctgctccat taaggtgctt tatactctct ccatctagtc tacaacccat
    10441 tctatctatt tcattcgtaa ctttgtattc atttgataaa aatttctcta cttcaccttt
    10501 agaaaaagca tcaaattgag gaccttttac aattcttagt acgatttctg aactataagt
    10561 tggaatatat tttgaattta attttttatt tattgtaaaa tctttttcct gaataaaagt
    10621 atttatataa tctccttttt taagtgctct accttcaaac ccaccaattt tagctcttgt
    10681 ataagttgac ttacttccca ttgcaagtgg aacatctatt ccaccagcta ttgacacata
    10741 ggctctagca cctgatttca cacctctaaa tgataataca tcacctggat ttatttgata
    10801 tgacctccag ttttgaattt cctttccatt tattttagcc cctaaatctc ccccagttac
    10861 tgctatttgc gtatattcat caaaggtaat tgtagggcct aacattaaaa cttctaaaga
    10921 agcctcatct tcagaatttc ctactaatat atttcctact ctatgtgcat actcatccat
    10981 tgctccacat ccagatacac catattgacc atatccaact ctacctctgt cttgtattaa
    11041 tgtttggaaa cctcctaatt caactttgaa tcccatatat accactccct gtactataac
    11101 gatatttaaa tacttaagtt atttttttcg atttctataa attcatctaa tgttattgga
    11161 acaaatttta taaagtctcc agctctaagc aaaatctgtt cttctctatt ttcatcatat
    11221 aattttaaag gtgtcctacc aattatttgc catccaccag gactatcaat aggatataca
    11281 ccagtctgag aacctgctat tccaacgctt ccaccataaa ttttggtcct tggcacttct
    11341 aatctaggtg tagctattct ttcatccata ccacctagat atggaaatcc aggagtaaac
    11401 cctagcatat atatcaagta ctctctactt gtgtgtattt ttataacttc atctacagtg
    11461 agattattgt agcttgcaac atttttaata tctataccaa aaacctcatc atagcatact
    11521 ggaatctcat gtattttagg ttttggtaat actatacttt ccaaattatc ttctattttt
    11581 tttaaattct caatcaaact atcaaaatct atttctaatg gattataact tatcataagt
    11641 gacctatatg ttggcaccat ttctgttaca atacctttaa gattcgatat ttctactgct
    11701 ctcataaatg aaattacttt tttgtttata tcttctgata tctcatttcc aaattcagca
    11761 accactgcct tatcccctga tatcaagtat ctagtttcca taaatgtcac ctcttctaaa
    11821 ttaagcaaat aaagcaccta ggctgctaag agaagttatt cctgtatatg cagatattaa
    11881 aactactatt aatccaaata ttaaaagcca tgtaggatgc ttgtaatctc caacaatatc
    11941 ttttctttta gatgctataa gcataattcc taatgtgatt ggaagtatca atccatttaa
    12001 agcccctgct aaaattaata aagtagctgg ttttcctata aatgccatta taagtgttga
    12061 tattgctata aatgctatta taaaatattt ttcatattta tctataaaag gatttagtgt
    12121 ttttaagaaa gaaactgatg tataagcagc tcctataact gatgtaattg cagcagacca
    12181 taatacaagt ccaaagaatt tatatcctat tgctcctgca ctaaatttaa aagctgaagc
    12241 agctggattt tctgggtcaa gttgaagacc ttttgaaact actgctagta ttgctaaaaa
    12301 taaaagaact ctcatcattg tggcaactaa tatacccaaa agagaagatt tagttatttc
    12361 ttttatattt tcttctcctg ttattccacc atctattaat ctatgtcctc cagcaaaagt
    12421 tatataacct ccaacacttc ctccaagtag agttattatt gggaatatca atccttttgg
    12481 attttctggt cttaccattc ttgatactgc ctctccaaca ggaggatgat ttgatattgc
    12541 tacatatcca actataatta tcattcctaa tgctaaaaat ttagttatct tatccactag
    12601 agaatttgaa ttttttgaca taaatacaaa tatggctatt aatccactta atacagttcc
    12661 taatgtcata ttcatattaa acattacatt cataccaagt gcagctccac caacatttcc
    12721 tatattaaaa gctaatcctc caagagctac taaaaaagct actaaatagc ctaatccagg
    12781 tattatttta tttgctatat cttgacctct aagtcctgat acaccaatta ctctccatac
    12841 atttacttga gcccctataa aaaggattgt tgtaattaaa attacaaatg aaaaattcgg
    12901 tccaaaatct tgggtaaatt gtgcagtctg agttaaaaac cctggtccaa ttgctgatgt
    12961 tgccattata aatgcagccc ctataagcgc tccaacatct tttttatttt tagttttatc
    13021 tgtaatattt tttgtactca tattttatct ccccctcatg ttatcagtct tatactatgt
    13081 tctctgatga acaaacttct atgttttcta atggacaaac ttctatactt tctaattcaa
    13141 aacgctttct tatttctttg acaaattcta ttgcttttgg attgtcccca tgtacacata
    13201 tagaatctgc ttttatatgt ataacttcac ctgttaaagt ttctacagtt ccttctttta
    13261 ccatcctaac aaccctatct attgcatgtt ttgtatcatg tataactgct ccttctacac
    13321 ttcttggaac caaaaaacca ttattatcat aagctctatc tgcaaatact tcatttgcaa
    13381 acctaagtcc aatgtcttta gcagaattta tcatttcact attatatagc cccaacagta
    13441 taatatcttt atcgacttca tatatagctt cacaaatccc catagctaac tctttatctt
    13501 tagctgccat attgtataat gctccatgcg gttttacatg ttgtatatta cagccatttg
    13561 aacttgcaaa tgccattaaa gcacctaatt gatattttgt ataatcttta gcttcctgct
    13621 tcgttatttt catctcacgt cttccaaatc ctattaaatc taggaaacct ggatgtgcgc
    13681 ctattttcac tccatttttt ttagctagct gtactgtttt ttccatatga ctagggtcac
    13741 ctgcatgaaa cccacaagct atattagctg atgaaatata ttttaaaact tcttcatcta
    13801 aaccaatctt atacgttcca aaactttctc ctaaatcact atttaaatct actttataca
    13861 aattatgacc cctccttgct aacattttga tagaataagt agttttaatt tttaaatttt
    13921 gtttatttta tatattgcat gaaatatttt atcacttgta ttcaatttta tttttgcaac
    13981 tattctattg catttaatta atataatttt accatttaag gaatattttt gcaattattt
    14041 taattaaaca ttcatttgta tttctttttc aaaaaatgtt tttaccattt tttagttaat
    14101 aattaattat tttcacttaa aacaaggggt ttaaccttca tttttaaagt atttttcatt
    14161 tttaatcact ttatatgcaa gacagaatca tttttcttca ttcatttgtt tctttcatct
    14221 atataattat cattctttgt aagtaaaata tctttatatt tttataatta attctttaca
    14281 tagttttaag tataataaaa tcatgtatac taaatgcatg ttaaaatatt attttaaaac
    14341 atgcacaata tttgtacata ttgtagacat ataaacctat aaattataca atagtattgt
    14401 taagattata tattttttta acataaattt tgtattgggg gaaaaacaat ggataaaaac
    14461 tatacgatgc caatatacca aaaaatagcg ttggatattg caaataaaat atacacgggt
    14521 gaaatacaag aggattcagt attatttgga cgctctgttt tggctggtaa atacaatgta
    14581 tctccagaaa cgataagaag agctgtcaaa attttagagg acattggcgt tgttaaaagc
    14641 ataaagggta aaggtgttat tgtactatct ccagacaaag cttcttcttt tataaaaaaa
    14701 tatagagata ttacaaatat atcatcatat aaatcaacac tttataattt aatagatact
    14761 aaatctaatt tagaaaatga aatattagat actataaata aaattttaga ctattctaac
    14821 agacttgaaa taatcaatcc tttagtgccc gttcagttca ccataaattc taattgcaaa
    14881 tatattggtc aaacagctgc aaaaactaaa ttttggcaaa atacaggcgc tacaatagtt
    14941 gcaattaaaa gaggtgaaga acttataata tctcctggac catatattga atttttagaa
    15001 ggagacattc tcttagttgt tggtgaccaa cacatttaca attctatacc aatgttttta
    15061 tatgaaaacg aaaaataaat tatataattt agtatgatta tataaaaatg caaaattaac
    15121 aacaaaaaag agcttcatat tttacataaa gctcttttta aattcaaata aatttaactt
    15181 tgattaattt ttaaatatcc aattcatctt ctttttccca gcttaaagcc ctcgatacag
    15241 cttttttcca gcctttatat aatttacatc tcttctcttc actcatattt ggattaaact
    15301 ctttttctac tgaccacttt gaagtaattt catttcttcc aatataaaat ccaacagaca
    15361 accctgctaa ataagcagct cctaaagcgg tagtttctac tattttgggt ctatctatat
    15421 ctacatttaa tatatctgac tgaaattgca ttaaaaaatt attattactt gctccaccat
    15481 caacttttaa tgattttagt tttaattttg aatcgttttg cattgcttct aaaacatcct
    15541 ttgtctgata agcaattgat tccaatgtgg ctcttactat atgttctttt tttgctcctc
    15601 ttgtaagtcc taaaattgaa cccctagcat acatatccca atacggagct ccaagacctg
    15661 taaatgctgg cactacatat acaccattag tatcctctac cctatttgca tacatttcac
    15721 tatcttttgc agattcaatt atctttaatt catctctaag ccattgaaca gatgctcctc
    15781 caataaatat acttccttct aatgcatatg ttacttttcc atcaactccc caagcaattg
    15841 ttgtaagtaa cccatgctta gatttaacta tgttactgcc tgtattcata agtaagaaac
    15901 aacctgttcc ataagtattt tttgcagtac cttcttcaac acaattttga ccaaataatg
    15961 ctgcttgttg gtctccagca catccagcaa taggtatttt agctcctgat aacatgcgct
    16021 catctgtatg accataaaca tagctagaag gttttactaa tggcaacatg cttattggta
    16081 tgtccaattc tcgtaatata tcctcatccc attctaaatc atttatattg tacatcattg
    16141 ttctagatgc atttgagtaa tctgttacat gtacttttcc acgagttaaa ttccatatta
    16201 accatgtatc tatagttcca aataataact ctccttgttc tgctttttcc ctagctcctt
    16261 ctacattatc taatatccat tttacttttg ttgctgaaaa gtatgcatct attaaaagac
    16321 ctgttttttc ttttattgat tcttccaatc ctttattttt aagttcatca catatttctg
    16381 atgtcctcct acactgccat actattgcat tataaatagg tttcccagta tacctactcc
    16441 aaacaaccgt agtctctctt tgattagtta ttcctattga agcaatttct tctggtctta
    16501 tacctgctgt ttctataact tctctcatta ccccactttg tgacccccat atttccatag
    16561 ggtcatgctc tacccatcca gcttttggat aaatttgatt gaactctttt tgtgcaattt
    16621 tcactatttc gcctttttta ttaaatagta tagctcttga actggttgtt ccttggtcta
    16681 atgccattac atacttcttt tccatcacca tattctcctt atcatataat atttaattta
    16741 ttaaaaagaa aaatttaata ttacatattt caataaataa tcatacttaa gtacaaattt
    16801 aatataatag atatttataa gaagacactt attttctatc atgtctatat aataatatta
    16861 tacattatag cttacatatt gattaataaa ttttaaagga gtataataaa cagtgttcat
    16921 aataatttta ataaaagagg tggtaatatg ccaaagattt tagaaaatgt aaaagaagat
    16981 atattaaagg tatctcggga tatgatttta gaagaagatt actcaaatat tagcataagg
    17041 aaaatagctc aaaggtgtgg tataagtgca ggtacagtat ataattattt taattcaaaa
    17101 caagaaataa tagaatatat cacaaaatct gaatgggatt tattgataag gcgaattgaa
    17161 tactccaata aaaatactga agattacata aaaaaactaa acattatttt tactgaaata
    17221 agaaatttta taaacaaagt tcataatatc caatacaatg actttttaaa tacttttgaa
    17281 acagagagat tttttgaaat gaaaaaacac aaagacgact ttcatgaaca gctttcaaat
    17341 aaagtatatg aagctttaga aaaagaaagt ttttttaata atgacaaatt agtatgcaat
    17401 atcataataa aaatattttt ttcatattca acatatcttc atatagaatt tgaagattta
    17461 aaaccctata ttgaaaaatt aatacattaa ggtggtgttt accttgaatc ataatactag
    17521 tattaagaac aaggacagta atccaaaaat atttttagta cttacacttt atctattagg
    17581 tatatttatg ggagctattg atactggaat agtatcacca gcaagaacga ttatacaaaa
    17641 tagccttgga gtaaatgaaa aaacgggaat ttggatgatt accatataca cattaacata
    17701 cgcttctgta attcctatct ctggaaaatt agcagataaa ataggaagaa aatatgtgta
    17761 tctagttagt atatttctat ttggtagtgg ttcattaata tgtggattat catcactatt
    17821 ttccaatttc tatattttat taattggtcg tgttattcaa gcaattggtg gtggtggaat
    17881 catgccaatt gctacagcag aatttggaac taccttccca gaaaataaaa gaggcatggc
    17941 tttaggttta gttggggcaa catatggtat tgccaatatt ttaggttcaa gcattggtag
    18001 cacaattcta agtatatttg gaactcaaaa ttggaagtgg ctattttttg taaaccttcc
    18061 aatttgttta atcatactta ttggcggagt tttctgtatt aaaaacaata agtctgaatc
    18121 taaagaaaaa atagataagc ttggtacatt aatgttgata tgtataatcg tatcactatt
    18181 atatggtctt atgaatatag acttttttaa ttttaaaaat tctatacaag atatatcagt
    18241 ttatccatac ttattgacat ttattgtact tatacctatt ttcatattta ttgaaaatag
    18301 agctaaagac cctattttaa gctttgaata ttttttaaat ccgagaatat tgatagtact
    18361 tattttatct ttaattgttg gtattggaat gatgggtatg gtattcgttc cacagtatgc
    18421 tgaaaatgca ttaaagataa atgctggttc tggtggatat tttgtagcta tattgggatt
    18481 atttgcaggt attgctgctc ctttaagtgg aagacttatt gacaagtttg gggcaaaaaa
    18541 aatactatta ctaggatttt ctgtatctat gataggctct ctttacctag ttttaattgc
    18601 tctaaaaacc aatactgtat ttagtgtatg tgtaagttta atatttatgg gattaggaat
    18661 gggatttact atgggaactc ctctaaacta tatgatgtta tcaaatacaa gattagaaga
    18721 atctaattca gcacttgcaa cattatcctt gatacgctca attggtactt caatttctcc
    18781 agctataatg attggattta tagcgcatgc aggattatct gtgcaagata atattatgga
    18841 cttagttgga aagccctcaa ctcctaaaat agttcaactt gaagaacttg aaaacatgat
    18901 agatgactta aaatcagacc ctgaaatggc aaaaaattta aaaaatgtct ctatcccaaa
    18961 tatgaatgat acttcaaata taaatatgga catgacaggt ggcaaactcc caaaatcctt
    19021 acttaataaa gttcagtcag cagatacaac caacattacc aatataacaa aagaaatatc
    19081 aacaacaatg tttgatgaaa aagttccatc tataattact aaaatacaga aaaacgtaca
    19141 aaaaggaata gatggaacac aaagtggaat aaacggtatt gaagacggtg aaagtaaact
    19201 aaatagtggt attaaaggag ttcaaactgg tattgacaat atggtaaaag ctagagctgg
    19261 tattaatcaa ggaatagatg gtgtaaaaaa aggtattgct ggtgttgata aaggtataaa
    19321 aggtatggaa caaggtcttg aaaaacaaga tgaggccatc aaggaactta caactgctta
    19381 taataaaata cctactacag aaaaaactcc tactgataac gaaaataact caaatgaaca
    19441 aaaccagagt aataccagta attctgaatc agacgacact aagaatgatt ctacagaaaa
    19501 taagaataac aattccagcg agaataaacc aaatgtaaat gaacaaaaag aaagtttaaa
    19561 tgctcaaata cagcaactaa aaaagacaag agatgactta aatacaaagt tacaaaaaaa
    19621 taaaactcaa aagaaagaat tgagtaaaaa actaagatca atggaatctc aaaagaaaga
    19681 gctacaaagc aagttaaata attctataaa caaaaaaaaa gacatggaaa aaagccttga
    19741 tacaatgcaa cagcaaaaaa aatcactcca agcagtttta gcaaagacac aagaggtaaa
    19801 aactgaaata ccaaaggctt ttgataaatc aaaattggat tatataaatt ccatagaaaa
    19861 taatagaaca aaaatagaaa acacactaca atctactcta aacttaggat ttaaacagat
    19921 gtatataaca gtattctgtg taaatctact agcatttata atattattgt tctataaaga
    19981 aaataaaact agataaaaga gtgttatagt attatattgt aaatttatct tattcttaaa
    20041 tattttatcc taaagaataa attcataaaa tttttgtaat tgattaatat aaattacaat
    20101 ataatactat ttttctctta atctttttct cttttacaga caatcaaaaa gcaatcttag
    20161 tttatcgaaa taaatttata caatctattc atacacccct ttacattttt ataacaatta
    20221 aacctattgt tatatataca aatcaaaata aaccatatat tttcaactat aatatgttaa
    20281 aatcatatta tgagcatatt aatttaagta aaatgtcatt tagactttaa tttagtagtt
    20341 ttatattata attttatata tttttagctt tgaatgtcat ttagacatta cccataaaaa
    20401 gaaaggagtg tattttgtgg atactttagg aaaaagaatt gcttatttga gaaactccaa
    20461 aaaacttact cagcgcaaac ttatggatat tttaaaattt gaaaacttag gcaagtatga
    20521 aacaggagat agaaaaccta attgtgatat actaatgtca atagctgatt atttcaatgt
    20581 tacgactgac tggcttctct atggtaaaga gaaggtaaat gtcaatagta gtgtaaaaga
    20641 agataaagaa gattacctac atgttactaa tgatgaaatg acgatattaa atctatacag
    20701 acaactaaac gaacgtgata aaataaaaat agaaggaata cttgaactta agatatctga
    20761 atataaagac ttaaaaaagc attcctctaa taataatgaa gataaaatgg tttaaacata
    20821 cataatttga tacgatttta catcatctat taaccttatt tttttgtagt atattactaa
    20881 aatatggata ttggattatt taaaaacaag tatagtctaa tttcaagcac atttataata
    20941 tttagattat cttatccata ttttagttta cgtactttga aaaatataat acatttccat
    21001 gtataattta tgttcctaag aaatgcatta ctcatgtttt taattcttcc aacatctttc
    21061 tatacacaat tgtacatcct tcataattcc cctctttaag aaggattgaa catgtactgg
    21121 aatatttagt taaagtattt aatctttcca tatttaagct ttttatatta ctgagtgctg
    21181 taataaaatc atcaattgct ctacaacact ctataacccc atctgaatcc aatttgcaca
    21241 caagaatatt cacctacttt atatactatt tattaatcat cttctgaata ttctatatta
    21301 agtaattgat aaattgatga tttatatttt ttacctgttc ttcttccatt taaaatagct
    21361 gacaaatagt tttcattaac acctattttc ttcgctaatt ccctttgagt catatttaat
    21421 tcaataagtc tttgcttgac ttgcactccc aaaaaaggta tccatttttt tcgagcatcc
    21481 atttatcaac ttcctttcta agtgatttat atttactttt atattatttt ggtttacgca
    21541 ccccctctat ttagtatata tttttaatat gtacacatat taagtatata aatttattta
    21601 atactaaata agattttttg attactttgt gctaaaatta ggatgtaaat tcgtatcatt
    21661 aagttaatta taaagtcatt ttgaccttaa gtcaatggtt tttatgtcat tttgaccttt
    21721 aatttgaaac tatttacatc ttaaaaagtc tttttgacat taattgaatg gagagtgttt
    21781 atggatactt taggagaaag aattgtttat ttgagaaaag caaaaaacct taaacaatat
    21841 gaactagaag aaatgttggg ctgtgacaac ttaagtaaat ttgagagaaa cattagaaaa
    21901 ccaaattatg aaatattaaa atctatagct gagatattta atgtttcagt agactggcta
    21961 ttaaatggag ataatctatc acataaatca gatttaattt gcgattcctc ctcaaactat
    22021 ccacttaata gcataaactc taatgaaata aagcttttaa acaactttag aaagttaaat
    22081 gattatgata aagccaaaat agaaggtatg attgaattaa aacttcatga atatgaaaaa
    22141 gagaaagatt taagaaaaac tgaatataac aaaaataaag atgagaaaat agataaataa
    22201 tttttatgtt gcttttgata cgattttaca tcatttcttt gttctaatat ttttatttca
    22261 tttattttct tttttataat tttatgataa aataattagt atattttgct ttacttgaat
    22321 gtaattattt atacaaaagt caaacataat ataaatgtag taaattttta gtttcaagta
    22381 atatctcgtt attataacta aatatttatt aaaattagga ggataaaatg ggcttaagag
    22441 acaaattcgc gcaatcattt gctagatcaa aaactatgag tggccctgag aaaaaggcta
    22501 atgaaataat gggtaaactg cttcttaaga aagctatatt acctatcgtt ttaatgttcg
    22561 tgataataat cgctggagct atgcttaaaa tcaattcttg ggttacactt ggcataaatt
    22621 tagttattgc tgttggtgct ttcttctaca taagaaattc tagtaaaaag tatcagaact
    22681 ttaaacctta tgttggcaat ttgattagtt tagagaaaaa aggtaaaaaa gagtacgttg
    22741 ctattataaa acaaggtaag ttaccagtta agttacaaat agcttatggt ggtgaagacc
    22801 ttgaacatgt taagaaaaat caaatggttc agataagtta taatccagat gctaaaatag
    22861 ctattttggt taatagacaa taatttaata aaaaaaggag ttgtcttaaa attgattttt
    22921 tctaatcaag tttaagctca actccttttt gaatttttac atgttatttt tatatattat
    22981 ttttattact ttttttaatt aatattttta aatattaaat ccttctcaat aaaatagggt
    23041 actatctaca atactacttt atcttttcct aacaatatat aatctggtac tactgctact
    23101 ttcatgttat acaccgtttt gtaacatacc ttttatatca gtaacatctt ccttaatttc
    23161 ctctacatct gttttcatta ttcccatttg aacaagtata ttcttattta tttcttgttg
    23221 ttgtgtagat aattctataa agttttctac agttttttta tacatgtctc tatcttcttt
    23281 tttctcctgc atagtatttt taaataatag agcacatagt atacctattg cccctagact
    23341 actcaattcg gttattaatt tatccattat agcctcctat tgtctaaatt ttttatactt
    23401 atagctttaa tttttgttat attcttacat catgcaatat tatattttca ggattatcaa
    23461 tttaatcttc tgtactttaa attgtaggtg taatttatat attgaataaa taataaatat
    23521 atatacaatt tctaatataa caaaaaataa gtacaaggct tacttattaa agttaagacc
    23581 ttgtacttta cataaaaaaa ataactaata catagttatt ctaaatttac tattcaactg
    23641 gtatttctaa ttctttacat ctaattctaa cttgctctct aattactgct ggtacttctt
    23701 ctattgtttt tttgcctctt aaaatcaaag ctacatatac ttctaccatt ccactctcac
    23761 ctccagattt tcttacacta aaagtgctta tattacttgc ttcattgaca gaactttgac
    23821 ttaacaacat ctcatatatt tctgcatttg ctataaaatt atttgttata tcatcatctt
    23881 gctttacttg acctatactt atgtctgaga tatctttata ctgcatttct acttttctac
    23941 cttgtttgat ttgttctatt ttatttttat cgaatttaaa caattttcac acctccattt
    24001 taagcttgtg gctcgttgtt ttcagcatca tatctaaaaa tcatagatgg acgttttata
    24061 gctgtccatt tatctccttt ttctactatg attttaggct gagcactttt tatgttggct
    24121 tcttctaatt catagatttc ttggtaactt gagtctgatt ttttgatttc aactagtttc
    24181 atgtctttga ataagtcaga tttatttatt tttacttgaa ctttagtttt atttattaat
    24241 tgatatatag ggtctccaag atttaaatct tcttcaagtc ctctatctag tgtaattgta
    24301 atatcttgtt cattggatgt tgtattaact accttgtaca ttctattacc tatcataaca
    24361 taagaatctt taattataga atttccagtg ctctctttaa aaataaataa agatgcatat
    24421 aaatcatcac ctttacaata agctctaaat aatatcttat tctctcctat tttaatttta
    24481 tctggtggta cttcaaatga tattttttca gtaattaaat cacttacaga ttttacactt
    24541 tcaccattta taagcatttc tattctactt atatctttat tagggtcttg tatttttatc
    24601 ttaattgatg aaattgattt ttcagtaatt tctatagcag catttgtcgt tcctacttct
    24661 gatataatat ataatgtatt gtctgttgca agtacatcct ttactgtatt tacatcttca
    24721 gaaataggta ttatagtaaa ttcttttaaa aattctactg gttgttcaat tttatataca
    24781 tccctatttt ttccagtaac ccatactgaa ttatcatttt taagtactaa tatatggtca
    24841 cttccataag attttatttc tcttatgtct gttagtgctt ttgtaaagaa cttaattata
    24901 gtattttcac tatcgcttcc taatcctaaa gctcccttat cattatatcc ttgtacaaat
    24961 aattcattac ctgtagttac tactaatgta tagtttttac cacaatgtac ttctttacag
    25021 ctagatatag ttgtaattct ttgtaattca gtaactggat tattgtgacc tatacctaat
    25081 tgatattcgg aatttttacc aatagaccaa agactatcat cagattttat aaagtaagta
    25141 aagttatctc cacatgaaac atgctttaca tctgatacat taatctgcac aaattcatcc
    25201 acttgattta tagacaagtc ttgcaataat tctccatggt agtttgtacc acatccatac
    25261 attgttccat cattaagtaa tataataata tgagtcttag aaatagatac ttcttttata
    25321 tcttcatatg gaagctcagt ataaaggtct cctttgtata aatttcctaa tactaaacca
    25381 taatctatgt cttttgtggt aggtatcact atcttattgt ttagaagtat aaagttatgt
    25441 ctgtttttaa tagatgtcaa atcacccatt acaaaattca tatgcttata attaaatatt
    25501 ctattatcat tatttctact atcgtatcta tttccaacac ctaattgtcc atagttattt
    25561 aatccacaaa tatatatatc attagtatct tttattgcta tatttgcata ttctaaagga
    25621 agtattttct ttactcttgt attggttttt ataaatgtat ctctttttgt tacatcacca
    25681 acacctaatt gtccattatt attaaaacct gtaccatata ggtctccatt atcacttaca
    25741 taatgtccac atctataatt cattccaatg tcctttgcct ttgatgctat ttttttaatc
    25801 ttctctggag tctcacttcc tgtacctgaa ccattactac atttacctgc agtatataca
    25861 tccccttcac ttgtaagtaa aagtactgca ttatttctgt ttggagctac ttttactaca
    25921 tttgaaattg gaatttcaga aaatgaactt tgattactat tactatttag acctaaacaa
    25981 taatcagtat ttagacctgt actatatgca gtattataga ttgttattat ataggtattt
    26041 atttcatttg gtataacttc ttttacattt tccatacact ttataaaact atactggtca
    26101 gtagtatttc ccatacctaa ttgtgaatta ttattgtatc cacaacaata tgcaatgttt
    26161 gtgtccttta caataaatgt tgttgtatct gagcagtata catctcttac attagttata
    26221 ttgtttacag taaatgtcaa tacgtctgga tggttgcttt cgcttcctaa tcccagttgc
    26281 ccatgagtat tttctccaca tgaatataat gtgttatcat ctttgattgc atgtgagtga
    26341 gtagtcccta aaacaacatc tcttacattt tgtatattta ctttggtaaa tgtgtttcta
    26401 tttactatat cacctaatcc cagttgtcct ttggtattta atcctgtgga gtatagtgtt
    26461 ccatcatttc taattgcaaa tgttgtgtta ccatctatac ttgttgatat tttctttata
    26521 ttatcaatgt ttatctgagt gaacttattt ctatttattg tgtcacctaa tcctaattgt
    26581 ccagaactat tatttcctgt ggcataggct gttccatcat tttttactat aaacattgta
    26641 tttcttgatg ttgtaatttc ttttatatca tccataaata taaatgagtt tgattgttca
    26701 gttatatttg tgaatttttt tattggttga ttgttatatt ctcctatacc aagtttgtta
    26761 aattgatttg ttcctgttac atatatattg ttgtttttat ctactactac tacagtgtta
    26821 ttttttacaa ttatatcttt tatatctttt aatcctgtat cataattgct tgcattactt
    26881 atactagttg aactcccaaa ctctccatta ttagattttc catatccttt taatacactt
    26941 tctccagaat aattttttag tatgaactca tttcctaatg atactgcacc attacagtta
    27001 tcgctcaaat aactagacca attttgatta gtttgtccat atgttaaaca acttcctgtg
    27061 cacctaaatg tagaattatt ttttaataac gataaaatat ttttactatt tcctacaaca
    27121 ctttttgaac tatcaaatac tgaacaacga attagtttat atgtccactg atagcaagta
    27181 tatactcctc catctgaatt tgtatgatat atgcctgaat tactactaaa tcttaattca
    27241 gattctggag ttatttgaga agaacttgta aatttcatat tattttcacc aatacaataa
    27301 gctcggtcac tttcatcaaa aaacatacat ccacgataaa aagcaaataa atttattaat
    27361 ttagtttgag ctgtattata aaattccaat gttctatatg acgtattcca agcattatat
    27421 cctttactct gatatgatga actagtacta ggtgctggat atccaccact accattatat
    27481 gaataaccta tagtctttgc agcaccagtt gaatctaata tatataaagt atcactccac
    27541 gtagctattt tttctacatt actaactcta gtatctttgt aagggtagtt tctattatca
    27601 ccataaacta tataagattg attaccccac ccatataatt cctttgaatg atttaatgct
    27661 acaatatagt tactttctac acaaaattcc ttaatatttt ctatctcaat ttttgtaaat
    27721 atatttctat ttgtattgtc ctttaatccc aatataccag aactatttaa accacaagaa
    27781 tataaagttc cattattctt aagaacaaaa gtcgagcttc catttccttt tatatccaaa
    27841 acattatcta tatccacctt agtcataact gacctgttaa cagtgtctcc caatcctaac
    27901 tgcccagcat tatttagtcc acaggcatat aatgacccat cattcataac aataaacata
    27961 ttattatctg ttaactctat cttcttataa tcctgaaaat tatcacttaa ctttacattt
    28021 aaaaattcac tattatcctt tgtaacatat aaataatcat cattaaccaa taaacctgaa
    28081 taattatcta aatttgaaga ttgtaccttt gaaaactcat acagaaaagt tgtatgaaaa
    28141 aatgaaccca gttgacctgc taaattacag ccagatacaa atatctcttt tgaaccattt
    28201 attaaaaaat taaattgaga accacactta atatctttta tattttctac attaacctta
    28261 gcaaaagtat ttcttgatgc tacatcttta ttggcattgg ctagttgacc ataattgttt
    28321 tgtcctgtac taaatagtgt attatcatat tttaatatca tagtatgatt atttccacaa
    28381 gaagcatact taacattatt tatactagaa agagtaaata caattgaatt attattattt
    28441 cctgtaccaa gctgaccaac tccattatag cctgtagtat acatagttcc atcattctta
    28501 ataagaattg tatgactact accacacgct atctgtttta cattatccat accttctatt
    28561 ttattaaata cacttttatt taaagtacta ttaattccca actgcccatt tgaatttaat
    28621 ccacatgcat gtgctgtccc atccattttt atgataaata cagattgatt ataagttact
    28681 acatccttta cactatctat atttacttta gtaaaagtag ctctattgtt agtatcacct
    28741 agtccaagtt gaccacttga gtttaaacct gctgaatata atgttccatc atttttaagt
    28801 acaaaagtaa agtcataacc acatgcaact gtttttacat catctattgg tagcttagta
    28861 aattcataat atactgtaga ctcaattaca cctaattgac ctacattact tgttcctgct
    28921 ccatatgcaa atccatcttt agttactgct acactgtgtt ttgaaccaca agatacatat
    28981 tttacattat caatttttac tttactaaac atccttctct ctgacgaatc ttttccaagt
    29041 cctaattgaa agcctgtatt atcaccacaa gcatataaat tcccatctga ttttaaacca
    29101 aaggtactgt tacttatacc agtaaaaaat tctacaaaat cataatcatt atatctttta
    29161 tcaaaatcat caatcaatat gttcttatca ttaaaataag caccacgttg taaaagttta
    29221 ttttgcttca ttattagtac tacccccttt atttatctta tttcatatga atacttattt
    29281 agagttatat ctttttgttc ttttaaataa gtgttgctag attttgtctc aacaaagtta
    29341 ttatccatat catgtatata cctaactcta tttgcactaa atggtaatac tacatctcta
    29401 aaaatcacat taccaatttc acctgttata cattttctat ttaaatctaa cttaagccct
    29461 tctatagcta ctatgttatt tccatttgct aatgtatcat accaataacc agcctcatct
    29521 acagttactt ctgactctaa ttctaggtat attaatatat ctagcacttt ttcagataat
    29581 ttgttatagc tattttttaa tactgatata tcagtttgta tttgaccact tataacatca
    29641 tcaaaagata tattttctat ttttttaact acctcattta tggctcctga taaatctttg
    29701 gaatatgtag ttaatatatt tacatcacca atgtttactt ttatttcttt tatattttta
    29761 tcaaacttat catctaaatc ttgaatatcc ttttgagtta caaaaaccaa cgtcgggtct
    29821 acttctaatg taacatttga agtattggat aactgtaaaa tcatttttac aaccaaatct
    29881 ttaatactgc catcttcagc acgtggctta tacgtctctg ggtacttacc tatagctatt
    29941 atattattct cattatccag aacagcagct tctcttatca taaatccacc aactgttcct
    30001 ggtaaaatag tctctataac tatccaatta ggattttttt catcaattct tagagaattt
    30061 atatttcctt cccaaactac attctttaaa gcagtttgtt cctctgttgg ctcataatat
    30121 tctccattac tatctccaac ttgaaacttg gttaatccaa caagctctcc tagtgcagtt
    30181 gcatttgcta ttttttctct accaattttg gttaatatta tataaaattt ttcttccaat
    30241 tataccacct ccgatttagg ataaactact acattttcta cgcctgtatc atttgcaata
    30301 gctatattaa tcttacaatt attttcaatg catttaatgc ttaaaggata aacctttatt
    30361 tcttctctac ttaacattcc agctccacag tttacaacag cactgcattt tataattggt
    30421 tcaaagtatg gatgaacttt tacctcttca ctacatacca tacctccacc acaatagacc
    30481 atacttttat ctagtattat tggttctaaa tagtgtaata agtgagcagg ttttactctt
    30541 tcaatcatat cgctacaatc taaaagagtt ttatagtcac agttatttgc tataaaactc
    30601 aatacgaatg taaattcatc actataaact tttatatctg tttctgattt tgtatatgcc
    30661 tcacatatac tttttataac ttcaatagta ctagtacctc tgcttctcat tttagctttt
    30721 atattgctac gtcttgtgtc aaaatcaagt tctttttttt caatgcagag tatatcttcc
    30781 cacatatcta atccccatgt tgcagtgtca acaaataatt ggttaaaggt atcatcaata
    30841 tcttctttaa gaatatttaa ttctgtatcg tatgcacctt gtatctcctc tacaatgtaa
    30901 tttctatcaa atgatggtag tttatcaatt agcttcattg attttccacc tcactaaaat
    30961 taacagttgt tacactaggt attttatctt cattaatagt tatattatct gtacttccat
    31021 ttataagtag gttacttaaa tcgtgtacac cagtagtatt tataagtatt cccattactt
    31081 ttgtatagat tatctctcct ctaatatctc taaagtatgt atttatactt tctaggaaag
    31141 attctttgac attgtctaat gtatatccat cttctagttt cattactgca cttatattta
    31201 tttctattgg taatggcgtc acaactgtta tagttggtcc aataggcttc tcttcatcta
    31261 tatgttgctg acaccttgca atcgtttctg tatcaacagc ttgattattt ttcccaaata
    31321 tcaagacctt aactgttcct ggaccatccc atcttggata aaccttaaca ttatagactc
    31381 catctacttc caaagcccat tcttcatagt gagctttatt tccacttgta gcttgatttc
    31441 tttggatttt ataaaatctt tcctttagct cttcatctgt ctctatatct gtaccacctt
    31501 caaaacttct tgtgttagta atctttgtta ctccagatat attatcaact agtttaaatt
    31561 cacaatttgt aggtaaatta tatttcttac caacttccag agcttgaact aggctattgt
    31621 caccttcttc actaccaata gttacatctt ttattactac aaatagtaaa tctctatatg
    31681 atattactgt gccattattt attacagttc ctttctctcc aataaattca acttctccat
    31741 ttgactctgt acctaacttt ctatatacac caaattcatt gactctttta tcgagaaact
    31801 ggttatatgt gtcttgtata aaagccattt tatgtatctt tgaaagttct agatatatct
    31861 tcgaaagttc tagattattt ccagatacca tgttgtttag aaaagaacct tcacccttat
    31921 aaatatcaag attaatattt tcaagagttc tattttttat tacttcatat gtctggtcac
    31981 tatacattta tttccacctc cccatagata gtggatactt ttacatttgc actcaatacg
    32041 tctctgttaa agttagcact ttttacgttt acttctaata tatatggatt tattagaaga
    32101 gcttctttta tgaatctact agcttcactt tctgtaagac cttttgtata tttttgtcct
    32161 attagttctg aaagctctgt cccatattct aaagagtata tctcatgttc atatctattt
    32221 gtctttatac acttgtatat ccaaactttt aaggcttctt ttttttcaat tattttaaag
    32281 tcaccttttt ctaaaatagg ttcatctttt tcaaaatccc atgccacttc acgaaagatt
    32341 ggcaattctt ctgttttagg taagatataa tcctctggga cacctataaa aggaaatatt
    32401 gtactcatta taagctcacc aacttactta caacggcaaa tttttcgcct attctaaaca
    32461 ttattaccat atccccagat tcaaagttat ctataaaagg attttttact tcatgctggt
    32521 gttcttgatt tgtttctgta tcaaatgttt ctagctgtct atcaagcatc caactatcta
    32581 tcaaaatatc ttctttttct aatatgatgt tatttgtctc tatttttaaa tctggcaatt
    32641 tactttttat ttttccaata aaaaaagaag gttgattatg atgctttcct tcttctctta
    32701 ttattcctat aaattcattt attggattag ccattatatc accatccttt tataatactc
    32761 tcctggctgt attaaaatct cttctactac ttaacttact tattttaact acatcacccg
    32821 tttgtggagc atgtaaaaat tctccattac ctatatatag tccaacatga ctgactggat
    32881 tatgaaaaaa tactaaatct cctgcttgaa gatttttttg ttctactttc ttgccttttt
    32941 tagattgttg atttgatgtt cttggtaaac taacattaac ttttttaaaa caatagacca
    33001 ttaaaccaga acagtcaaaa ctacttggtc catttccacc ccatttataa ggtttaccta
    33061 gatgtttttt tgcttctgat attactattt tagctttatt ggatgcatta ccagaataac
    33121 caccgataat aatttttcct tttctttttc caaatctttt catttcttct gcatttttta
    33181 ctaaaacatc gatatgataa gttccatctt tttctataat tatagcagaa ccattatcat
    33241 ttactttata gactccatca cgtccagaaa ctcctgttac aatacttact tcatcaccat
    33301 atttaaataa tgggtgtttt gataggaact cttttgtata ataagatttt tcataagctc
    33361 caaccatagg tgcagcacat gtctttttag aaggattaag ctttttctct ctacagtctg
    33421 tatctccacc ttctatacca ggtccaggat aatatgctgt aaatatagca gaaaattctg
    33481 tccctcctac ataatcttct cctcctaaat tactttcttc cttaggttca tcctgtcctg
    33541 ctgacttttc atccataaga ttttgaaaat taagttcaag ctcgatttga tactctccat
    33601 tttgccaagt atgtttgtct gtatctatat aaaatagtcc tacaagcttt gtataagaat
    33661 ctttaacttt tactcctcta ccagttatac aacttacatc tccataacct ttaagagaac
    33721 agcttttttc tatcccatta aactcgctat caatatctac atcttgattt tcttgttgct
    33781 gaattacttt ttgcattatt acatttactt ccttaaaaat ttcattatct attttttcgc
    33841 taatcttact tccatactgg tctaccacta ttactttatt ttttacattt tccatactct
    33901 ccgaaaaggt ggtatttata atattaaatc cctcttcaaa cataacactt aatgtaacag
    33961 ttcctttttc aataacatta aacttatcta aattagcctc tatcatatac tttttctttg
    34021 tctttttact tgcctctgta tatgcactca ttattgtgtc ataaccattt actcctataa
    34081 acatctttgt atacttgaca ttggtctttg ctattgttcc aactgaaagc ctattttcag
    34141 caaatacttg ctttgctatg tcttcaacta acttatcttt aaaattgtat gacacttcac
    34201 tttgtgtaag taaaaatccc atatctttag atacaaaact aatttcatta ctgcttgaat
    34261 ctttagacct attaattatc attcctctaa agagttcttt ctcatctaca tagaaacaga
    34321 ctgtactagc tattggtata tctatctgtt ggaaatttac atcaaatgat gattgtatta
    34381 ttgaaaactc tagtgtcctt gatggagatt tataatcacc tgaccatgat accttgtcta
    34441 ctatgtcagt tatgtcatat atacttccgt tttttatgtg tacccaaatc ttaataataa
    34501 aaaatcacct gccttttcat ttaattctat aagcagttat ttataactta ttatctataa
    34561 tagttattgt tttggagtta cttttggtat ctttattctt ttatactctt ttaaatctaa
    34621 tgtaaaatat acatctcgag tcccatctct ttcttcataa ctaaaccctt caattatgac
    34681 ttccatattt atatttgttt ccgtaattgt aaatcttaat ataaaacctt ccttcatcca
    34741 tttttctatc ttatttacac aatcatatgg tgatggaaaa cctgtatagt cacagaaagt
    34801 gtagtcttta tttggaaaaa aacttgatat tgaaatgctt ttaagagcta caccaccaaa
    34861 agttgctatt tcacctattt tgagtatact agaagagttt acagcagctt ttccattaat
    34921 actaaaggaa gatggaaata ctggaaatct aaatctatct tctgcttgtc taagccacat
    34981 ttccactata atacacctcc caattgtgcc atttttatac ttgaaactaa ttttgatgta
    35041 actttttcta tatcagcttc ttctctaatt actattgtat cagctaactt ggctatactt
    35101 atatttccac cattttttcc acttctatat tgatttgctt cttgtttagt taaaaccatt
    35161 tcaccctcat gaagccttgt tgggtaatca ttataaggaa catagtttaa tccaaaggcg
    35221 ttacttccag atttgcttgt acctgcattc caatcatcaa ttatagatct aactgtctca
    35281 ataattggat ttttattaat tttttcaact aaattttgcc accatttaca aacatctttt
    35341 ataatattgc atatatcatt aaatagatta aaaagacctc ctaaaactgg ttctacaata
    35401 atccaagcat cctctaaaag tggtcctatt gttgcccaaa ctgtctccca gataacacct
    35461 agtgtctcta taaagttatt tatttgaggg gcatattcac ctaaaaaagt aaaaattctt
    35521 gtgataatag gactaacagc ttcaactaca ggtccgatta gttggaatgc tgtagttact
    35581 attgtaccaa atatacttat taatccttgt gcaattggtg atttattgtt tatactatct
    35641 agtaaattat ttactaagtt tactactgga agtaatgaat ctattaatgc tgtaaaatcg
    35701 atattttgta atagtgtact tcctgcgtta acaattgctt ttggtgcatt ccctatcatt
    35761 gttgctgcat gattttcaaa acgttcttgc ttattttcaa ccatcatttt acgcttttca
    35821 gcctcattag aaattcgata ttgagtagaa tcagaaaact cttttccatt ttttacactt
    35881 ctagattgca tctggtcata tttgttgcca ataattatct taccattcat tacattgcct
    35941 attttaccag ccattctttc agataatcca gctaaattgc ctaatccact ttctataaaa
    36001 gtttttattt tctttagtcc ttgacctaaa atatcatcaa aaggtttaac aatactcttc
    36061 aaaccattgt taatgcttgt ttgagctcct gatattatac ctggtattgt attactcatt
    36121 tctttagcta accccatctg ctttacactt ataccttcca atgcttcatc aaaagtttta
    36181 tagttttctc ctaccagagt attaagagat tccatatttc cttttgctgc acttaagaaa
    36241 gctgctgata catcttgttc actacttgta ttcatattta aagctctaac atccaccata
    36301 gcctgagtta tatctttttc ttttttacca ccagtaatat tactaatcat tttagcttta
    36361 gttcccattg caacaacatc ttcagggtca aaaggcgtat catttgccat tcttactaaa
    36421 ctgccataat aatcatcaac ttctctttga ttaacttttc cagtatttct tttcattaca
    36481 ctttctagaa caactttctg attttgttcc tgaccaagta aatcaacacc ctttttcaca
    36541 gcgacaccta tagtagcacc atttgtcaaa tcagatatct tcccttttat cgaaccaagt
    36601 attccacttg ccatatcctt tacagctatt gcaccactcc ataccttacc tgcaaataat
    36661 ctcaatttgg gaaatacttt acttataatt ccacttgcca tatcttttat agctattaca
    36721 ccactccaaa aagtattttt aaacaacatt aacttaggca aaattctacc catagcacta
    36781 ctcaccatat ctttaagatt aatacttcct tgataaggac gtttctctac ttgttttaat
    36841 tttcccaaga tactacttat aactctctta gtcatatctt ttgcttttat tacaatcttc
    36901 caaaccttgc caccaatctt ttttaaattt ccaagtaatt tatttttagt tttttctatt
    36961 tcatcacttt tagctttaaa tattaatata atcataatat ttgaattaaa ctcatttttc
    37021 ttttgaatca acttattaaa ataatcaata atcatagcta tagttattgc tagtctcata
    37081 atacttccat ttaaacttcc aatcactcct gaaacagcat ttatgctaga acttaatttt
    37141 gtcatagttg aacttacact actagcagta gtttgaactt ttcttaacga agaatttaaa
    37201 ctatttaccc tactacttgc acttccaata gaatttttta tgctactagc cattacatta
    37261 gtggaaagtg aaacagccac agttgctctc ctaaaattat cagttactga tataatttga
    37321 ttcatttttg gtgtgtaatt atctgtaaga tacatattta taccttcttc tctcacattt
    37381 cccattacat accacctcct gtcaaaggac acattccata tccatgtttc attttttcaa
    37441 cttcttcctc aatttctaag gcgaaaaaag cttcaataag ctttaattcg cctttatcca
    37501 ttgcataaaa aagggacggt cttatacctt ttttcttcca ataatagtac attattgtag
    37561 tcctggtatc cgtccctatt agttttttac ctcttctatc actgcatttt taccatagcc
    37621 cataacatct tgaagtattc tatataagtt ggtaatttca cctggtaata ataattttct
    37681 tgctaattct tttggtgttg gagccttaaa tttatccatt agctccttat ttttaaataa
    37741 aagctttcca tctaaatcac aaacaccttc tataattgta agcatctgca tttccggcaa
    37801 gtctatatcc gttttcatat tttcatcaat tttaagacaa gtattttgta tttcttcatc
    37861 tttacttatt gttaaagctc tacacattac tttaaattcc tgcccaaata tagttgataa
    37921 tctcttaagt tcaactatag tactcggtct ttctaagttt tctgtatctg aatttaaaag
    37981 taaatctact atgtttatat tttctgataa tccattttca ctcattatta tttgctcctt
    38041 tatcaatcaa attcaatcaa ctaacttttt tacttattaa ctatctgaat ttattaagta
    38101 tatatttact aaatatcagt ataaaaccaa atataaaact aaattatatc aagaaaatca
    38161 tactcagtaa atgtgaaagg tgcttctatc tctcctttta cgccaacctc ccaatcagct
    38221 aaagttaaat catcaaatgc tatattcttt actgctattc tttctgcccc ataagaatct
    38281 gggtcattta attttgatat agcaacaaat ctaggttcag aacctctctt tatcttttct
    38341 ccaattaact tgtgcattct tgaactaaca tggtatagag ttattgaacc ttttcctttg
    38401 tatcccatat attttgtatc agtacccatt tgacctgcta ttataatatc ctcttttgta
    38461 aattccatct ttgcttgaaa tttctttact tctgctactt tgtttccatc aagccaaagt
    38521 tctccccaag taccactcat tacatttcta gcttccatat ttgccatatt aataatcctc
    38581 ctatatttct attgataaat ctatatcttc catagcatca agtactttta tttttgcttt
    38641 taaaaatact ttagaacctg tgttagcttc ttttatttct tgtaatgtca tataagataa
    38701 atctactcct tttgatttta aatacgattt ttgtgcttca aaatctattt caacagtaga
    38761 atcagattct ataagtgcag atttttctaa ttcttctaaa taacttttta tagctactat
    38821 caataaacat ttgttgtcat aactgttagt aacctttcct atatagtcat ctattatcac
    38881 ctttcttatg tcactatgta taatatctaa agtgtcaact atttttattt tctggaacat
    38941 ttctcctttt tcttctgtta attcagttaa agaatttact cctctagcaa ttcttatagc
    39001 tcctgcttcc ttaataagta taagctctcc tttattaacc cttgattctg catcaacttt
    39061 agtcatctta ggtatatcga ctacatcaat aagcttagta taagttactg attgacttaa
    39121 aggtgtacct gctataagcc cagccaccct acttgtaaac tcatcaacac tgtatttctt
    39181 ttctccaact aacacatctt ctgtagtaaa attaattatc ccttcatgat ttcctacaac
    39241 ttttcctaat acagctttaa ccttaacctt atctatatct ctaagtttaa ttatccaatt
    39301 ttttatagca gtcttatcag cttctactgc ttttggcata catagataat taaattcctt
    39361 agtctctaaa aaatctaatg cagtttgaat atctgagtct ccttctatta cataaactaa
    39421 taatttattt ggagtgttaa catttcccat caaagctaaa tttatatatt ctttattttc
    39481 agcagataaa tcaactggta tatcctcttt ttcatgtatt tcgttaagac ctaatgcctt
    39541 agcgtcttta agcaccattg caattattcc tctagctgaa cgttctttaa cagttgtagc
    39601 tagctcctta aatgatatgt tgatacttgg taatcctata gccatactat cactctccta
    39661 aatttaaatt gatttcttcc atattttcat atactacatc ttcttcatat acactatcca
    39721 gatagtttag tgtaaccgaa aaattcagtg cataaattga ctcttctttt ttaatacttt
    39781 gttctagctt tgttatattt aattttctgt ctgttacctt tatatttctg ttgaataact
    39841 tatttaattc atctgaaatt tcatacattc taatactatt tgactgtggt tttggtaagt
    39901 attttataga aataacaata tctttattat tcgtatttaa cattataggt gtacaaataa
    39961 ctgatagtat acttacaaaa aaacaagacc cttcagagcc ttgcacactt tcatcatcaa
    40021 taaaaatact ttcagtaaag ttttttttga gaatctcaat aattgtttct aatatttcct
    40081 tgtatttaag caaataatca ccacactatc actaaatatt cttgcttaat ctgataatct
    40141 tactataaat ggtataagcc atttttaaat atacatctca tttattcctg tcaagtatct
    40201 tctatatttc taggtaatat tattcttttc aatttatcac ctccttcaat atggatttct
    40261 ttcacaataa cattatctca catattttat tgcctattgt tgcaatggtg tatataaagt
    40321 gttgtaaaat tgtcctattt tttataatat ctcactcagt ttaaatatca tctttttctt
    40381 taaattgtag caagatgttc tatctaagtg tagtttcata gaaatatatg tcatattgtt
    40441 ttttgtctta ctattataaa aaagattaaa aaattccatt tctatatcat ttagacatgt
    40501 tagagcattt tctatcttct ctttttctat ttctatatct tctttttcca tttttaattt
    40561 tcttaatctt tcctctcttt ttaatacctc attttccaca gaagaactta tgttatatgt
    40621 gttactagtc ttttctgtat ataccattgc tccacatcca gagtattcat tctcacatct
    40681 ttttatgtcc aagttaatat tctttatctt tatatccaaa tacttataat tatgtaatct
    40741 tttatctgtt tctttaaata atatatcttt tttgttttcc atagtacttc tcctttttta
    40801 tataattatt attagcttca aatttacaat aatctcttta gtgcacaatt ttttactaat
    40861 ctcacagctt tcaagaacat attgttctat aactgtataa aaaaaacttc ttctatattt
    40921 tgttctaaaa tactagaaat cttcttagcc ataagaagtc caggattttt tatacctctt
    40981 tctatttgag atatatagtc atttgtaatt ccagcctttc tggcaagttg aatttgtgtc
    41041 atacctttgc attttctata atatttcaag ttatttttaa acattgtttt atacctccat
    41101 tactattaag aatattttgt tcttccatat ttagattata tagaattatt tgttctttat
    41161 caatagattt tagaaaaaaa tattctttta tattttatta gttttatttt ttagaacaat
    41221 ttgttataat ctatacataa ggtggtgtta taatgtttgc caaaagatta agagaactta
    41281 gaaaggaatt tggattgact caaagagaac ttggagaaaa agtaggcgtt tcccaaagag
    41341 tcttagggta ctatgaaaca gaaaacagat ttcctgatga acacatttta aataaattgg
    41401 ctgatgtatt taatgtatca gttgattacc ttcttggaag aaccttagtt aaggaaaata
    41461 ttgatacagt agctgcacat agaaaaaatc cacatgaaga gttacctgaa gaagcacaag
    41521 aacaacttaa tgattatata gaatttttac taaataaata taaaaaaaaa taaaccatat
    41581 gatgagcagt ctatttttct gctcttttaa tataacttaa aggcaaacat acattctatt
    41641 ttagggggat ttttatgaat aatttggata agctttttga attagcttct caagaagaga
    41701 taataattca ttataccact tatattgcag gtgatttaga aggactatat ataaacaaac
    41761 atggtattaa aattatatca ttactcagta atttaaaaca aaactcaaaa aaattgacat
    41821 ccatcttagc tgaagaactt gggcatcatt tcactagcct tggatactat gtatcctctt
    41881 ataatgatta ttatacaaaa attattatag ataaatgcga aaataaagcc ttaaaatggg
    41941 cttgtgagtt cttaattact gaagaagata taataaacat aattaattct ggcatcactt
    42001 gcgtttatga aatggcggac atacttaatg tggatattac tttctttcaa aaaagattag
    42061 aatttttatc attaaaaaaa cagtctttac aacttggaaa taataaatat ttaatattaa
    42121 ctaatttgcc gtatttctac atatttgatc caatttcata ataaaaatag tagtaatttt
    42181 aactggctta acagtaaaat ctatatattt attttaacaa
//
LOCUS       DAEMAE010000010        37752 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEMAE010000010 DAEMAE010000000
VERSION     DAEMAE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.010"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..2088
                     /locus_tag="KPH93_000461"
     CDS             169..2088
                     /locus_tag="KPH93_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1687298.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISAEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKVDLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            2101..2388
                     /locus_tag="KPH93_000462"
     CDS             2101..2388
                     /locus_tag="KPH93_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1687299.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVEVYPVKKYMDLAEVTEAVKTFLSDKNDKK"
     gene            2521..3780
                     /locus_tag="KPH93_000463"
     CDS             2521..3780
                     /locus_tag="KPH93_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG1687300.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFVGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAILSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            3783..4523
                     /locus_tag="KPH93_000464"
     CDS             3783..4523
                     /locus_tag="KPH93_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1687301.1"
                     /translation="MKFKYIDEIRRLIDVIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVIINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNRGLKNPPI
                     FYSANIDGGDELNKKIFDEYKDVIHYEY"
     gene            4846..6156
                     /locus_tag="KPH93_000465"
     CDS             4846..6156
                     /locus_tag="KPH93_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG1687302.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGTGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKDVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            6254..7171
                     /locus_tag="KPH93_000466"
     CDS             6254..7171
                     /locus_tag="KPH93_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG1687303.1"
                     /translation="MENLVREFEENSRRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLVYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            7187..7996
                     /locus_tag="KPH93_000467"
     CDS             7187..7996
                     /locus_tag="KPH93_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG1687304.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFLNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            8054..9802
                     /locus_tag="KPH93_000468"
     CDS             8054..9802
                     /locus_tag="KPH93_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG1687305.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKNKDLGIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAQEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     LLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNC
                     GYDKVKKYVLELLDRV"
     gene            10064..11086
                     /locus_tag="KPH93_000469"
     CDS             10064..11086
                     /locus_tag="KPH93_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG1687306.1"
                     /translation="MFGGIKIKRIGIIIMFFLSIAFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDFDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMEYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            11091..13397
                     /locus_tag="KPH93_000470"
     CDS             11091..13397
                     /locus_tag="KPH93_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1687307.1"
                     /translation="MKKNSKKILITLTIITNIIYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSERRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNIRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSITYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIELEINEGIVLECDLCEGKIDERGILYRVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIFVSKPSEKKEKSIDEFEPMEYI"
     gene            13369..14484
                     /locus_tag="KPH93_000471"
     CDS             13369..14484
                     /locus_tag="KPH93_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687308.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSVQSKNMLEFRLKEGSEIFLEDKNIVYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSKEKL
                     VFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGKIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            14502..15920
                     /locus_tag="KPH93_000472"
     CDS             14502..15920
                     /locus_tag="KPH93_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG1687309.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKIFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELVNIELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQVKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            15936..17144
                     /locus_tag="KPH93_000473"
     CDS             15936..17144
                     /locus_tag="KPH93_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687310.1"
                     /translation="MNRFFKVRLITAIMFLIVLFAFSIFNICYSGKDMIQEIKNLVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSSVKDTSGKMHYTY
                     FAKAPNPIGVISDRVRKFSEEMEKQNTKLVYLMTPDKYIQGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSILYPKSFLGSMGRKSGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKFQKFELKGRFEESLVLSYPFNADLNLMDGQSDKYSTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISIYPQN
                     LTKEFFPFCE"
     gene            complement(17225..18613)
                     /locus_tag="KPH93_000474"
     CDS             complement(17225..18613)
                     /locus_tag="KPH93_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG1687311.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGVIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            18955..20289
                     /locus_tag="KPH93_000475"
     CDS             18955..20289
                     /locus_tag="KPH93_000475"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG1687312.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEITDVLEEEINRHNVNLHLDEMVVELY
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINIKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(20418..21335)
                     /locus_tag="KPH93_000476"
     CDS             complement(20418..21335)
                     /locus_tag="KPH93_000476"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG1687313.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCVRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICESMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            21543..22427
                     /locus_tag="KPH93_000477"
     CDS             21543..22427
                     /locus_tag="KPH93_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG1687314.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            22640..24481
                     /locus_tag="KPH93_000478"
     CDS             22640..24481
                     /locus_tag="KPH93_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG1687315.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLDRFEEITIFHTNDVHGRY
                     AEGDDHIQMGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVSELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEK
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKNVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESTVIEYIEYKKEISKNVDGRIGIK
                     SKDELIRKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFLVSSTLICLLNFNQKELKDKKSK"
     gene            24786..25493
                     /locus_tag="KPH93_000479"
     CDS             24786..25493
                     /locus_tag="KPH93_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687316.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFIKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFHYIENKLIIKTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDNSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            25487..26797
                     /locus_tag="KPH93_000480"
     CDS             25487..26797
                     /locus_tag="KPH93_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1687317.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIGYSILSVLIVTLVFILFEYSR
                     LHKLYEYQIKNTQNIAGILIEKYPNDEIDIVKSIYNSDYSKVSIGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIDIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETSYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQINGEINISVEETANYFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRRILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            26914..27597
                     /locus_tag="KPH93_000481"
     CDS             26914..27597
                     /locus_tag="KPH93_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687318.1"
                     /translation="MKILYTENLSKHYGKGESLVKALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            27590..29977
                     /locus_tag="KPH93_000482"
     CDS             27590..29977
                     /locus_tag="KPH93_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1687319.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNKDALKLNSFEFVKGKLPIRENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISETYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQVKEKLNKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     LFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKNK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAVYYTSGMMIGSILFGGILGYIAV
                     ILLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            30257..31399
                     /locus_tag="KPH93_000483"
     CDS             30257..31399
                     /locus_tag="KPH93_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG1687320.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDKDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHILNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            31422..31946
                     /locus_tag="KPH93_000484"
     CDS             31422..31946
                     /locus_tag="KPH93_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1687321.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIV
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFVVPLTSIPGNLMQVLIGLI
                     IALPISKVLRKCIK"
     gene            32046..32864
                     /locus_tag="KPH93_000485"
     CDS             32046..32864
                     /locus_tag="KPH93_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1687322.1"
                     /translation="MLSVTRYSIKNIRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQYGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAISKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKSV
                     ELLNSFIYVNRYSNIKAFCDDTNINIKTLERTCLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            32942..33253
                     /locus_tag="KPH93_000486"
     CDS             32942..33253
                     /locus_tag="KPH93_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG1687323.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(33383..34066)
                     /locus_tag="KPH93_000487"
     CDS             complement(33383..34066)
                     /locus_tag="KPH93_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687324.1"
                     /translation="MQKEAIMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLDNYLLRPILDLNKQHIEQICAFAIKPDC
                     SFIELKKVEDGNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSYNIPYMAPEVVLFCKSIYIDRVGYQKDFDVTVPLLSQEQRDWLVKALITAYPDGHA
                     WIDQLANQY"
     gene            complement(34158..34799)
                     /locus_tag="KPH93_000488"
     CDS             complement(34158..34799)
                     /locus_tag="KPH93_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1687325.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYDVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(34973..36976)
                     /locus_tag="KPH93_000489"
     CDS             complement(34973..36976)
                     /locus_tag="KPH93_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG1687326.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGWFYREWAPNADSLSLIGDFNNWNRKSHLLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILSKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYRNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEINPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(37129..>37752)
                     /gene="recX"
                     /locus_tag="KPH93_000490"
     CDS             complement(37129..>37752)
                     /gene="recX"
                     /locus_tag="KPH93_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG1687327.1"
                     /translation="KIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEINEEE
                     LKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIVERVIDFLKSYNLVN
                     DSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTIDEAVSELDKDTEFENAMYLAK
                     KRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        14584 a         4164 c         6380 g        12624 t
ORIGIN      
        1 tgtataaata atataggcta ttttcaaatt tttttaatac ttttaccact gtattctttt
       61 agattagagc tagactgtgc aaaattataa aataatacta aatcatgtgc aattatttgg
      121 tatggcacta catatagaaa atatagataa taaaattaag gaggtagtat gaaggaaagg
      181 gctaaaaaaa tattagattt tattattaaa aataacaatg taacatcaca agaactacaa
      241 gaaaagttta atgtttcaaa gagaactatt tattacgata ttctagctat aaataaacaa
      301 ctaggaaaaa gtggtaatat aaaaaatgta aaacacaaat ttatatttga aggtaattta
      361 tgtgatgcaa gaaaaataat ttcagctgaa gaagataaat ttttagatag tgattacaga
      421 aaaactttta tattaaacaa gatattactt ggcgaaaaaa tatcaataga aaaactaaca
      481 aatgaaatgt tgctttcaaa aaacacagta gtacaaacaa taactgatgt aaaaaaatac
      541 cttcaaacaa tggggttaag attagagtat aagggaaagt acaaaattat aggtgatgaa
      601 tacgttatta gagagctgtt tctgataatt gttcaagaaa atgtactgga aattaattct
      661 ataagtaagg aagtaagtag tttcgacaca aaaggacata taaaacttac tgattattct
      721 ttattaaatc taactaagtt tgtggaattt ctaaataaaa ggataagaga tgggaaaaca
      781 ttatactcat ataaatatct aaatgaagct aaaaaaatta gttactttag taattgtaaa
      841 gaacttttat gtgaagaagc taatgaaaat gaacaagcct atatatgcac ttacataagt
      901 tcacttccca gcttaaatag tgaagttaag gaagatgttg ttgaagaata tgtagataaa
      961 cttatagata agtttgaagt aaatacagca ataaaattag aaagtaagca tgagtttaag
     1021 aaaaatattt taaggcacct ccactcttct tacaacagaa tacgatttaa gtttcctatt
     1081 agaaatccaa tgttagatga gactaagtat aaacatgagt cactttataa aattataaaa
     1141 tcaattatag aaaacgaaga agagtttcct gtatttgagg ggataagaga agaagaaata
     1201 ggatttatag cagcgtattt tggagggtat ttaagagggt ctagggataa tggtctaaga
     1261 agaaataaag tcttactagt ttgtcctaat ggtcttatgg tatcaaagag tttggaaata
     1321 caactatata aatacatacc aactatagaa atagtaggta tagtgtccat taagcaacta
     1381 aaagaagtaa atgtatatta cgattacata attacaacta tagatataca aaatgtaaac
     1441 aatgtaattg ttgtaaatcc tcttttaaca tcatcagatg ttcaattact tatgaacaaa
     1501 ctaataagtg taaaagaaaa tgagaagtat ttcaatcttg aattaataat acaagctata
     1561 agaaaaaatg gagttatcaa taatgaagaa gcactaaaag ttgatttatt aaacataata
     1621 cacaagatag atgaaggaga gatgtatcaa ccaatgttaa aggaattaat taatgcagaa
     1681 agggtcaata taataaaaag tgttagagat tggaaagaag caataaaaat agcatcaaag
     1741 ccattgttag aagataactc tatagaagag ttatatatag aaaatatgat aaaatcagtt
     1801 gaaaaatatg gaccatatat tgttttagct gatagatttg ctctaccaca tgcaagttct
     1861 aaagaaggag taaacaagct tgccatgtct ctattgatag tagaagatga agttgattta
     1921 ttaggaaaac cagtaaatat atttatggta ttagctgctg tagataacac tactcacatt
     1981 agagcattag catcactatc agagatgatg tacgaggaag aaaatgtaaa actaataatt
     2041 aatggtgata aaagttcaat actagaactt ataaataagc aaaattagga ggaatgaaaa
     2101 atgaagatat tagcagtatg tggttttggt gtaggttcat cgatggtatt aaaaatgaca
     2161 atacaaaagg ctctaaagca gttaggtata gaatgtgaag tagaaaatac agatataaat
     2221 tcagctagag gcgctgactg tgactgtata ttcacaagtc aagaattgct aaatgaatta
     2281 aaaggaactt gtaatgtaga ggtatatcca gttaaaaagt acatggattt agcagaagta
     2341 acagaagctg ttaaaacttt tttaagtgat aaaaatgata agaaatagta taagttttaa
     2401 aaagtaaatt taagttaatc agaataaaat aaaaacttac taaatttaaa aatagcaaga
     2461 aagagaggag ggatttagtg gtagaaaact taaacagttg acctatcact aaaaaatata
     2521 atgcttgatt ttatttcaaa taatatttta cgtaatccat ctatgttaat tggattaata
     2581 gcagcatttg gtcttgtatt acagaagaag aaagcatctg atgttataaa ggggtcacta
     2641 cttgcagctt tcggaattat aattatggag actggaacag gtatgttagt tagttcaata
     2701 gcaccaatta atggagcctt ccaaagtata agtggtggtg cagtaacaaa aggtttaagt
     2761 gatgttactt ttactgcaag ttatggtgga attataggtt tagcaatgtt tgtgggttta
     2821 gctattcacc taatgatagc aagatttact cctattaaga caatcttctt aacaggtcac
     2881 atgctttatt ggttcccatt catatttgta gcttcaggag ttgaagctgg attaaatggt
     2941 ggagtattaa tagcttttgc agcaatatta tcagcactct attggtcaat aatgccttgg
     3001 ttattaagaa aatatgtgtt tgatgttata ggagatgatt cttttacatt aggtcatcca
     3061 tctggttgtt tagcacttat atcaggattt gttgctaaat tagtaggaaa taaagaaaaa
     3121 tctacagaag atataaacat acctcaaagt ttatcatttt ttagagaaat atctataagt
     3181 ggtggaatgg cagtgttttt aatgtttata gttgttggga tagtaatacc aggtgcgttt
     3241 aaagatagta atgagtttat tggaacgtat gcaataaagc aaggtctttc ttttggagca
     3301 ggtcttataa taatgcttca tggggtaaga atgctaataa atcaaataat gccagcattt
     3361 caaggtatat ctgaaaaagt tgtgccaaat gcactacctg cttttgactg tccaatatta
     3421 tttaactata aaccaaatgc tgtattaata ggattcttag ttgctatgct aacatctaca
     3481 gctatgattt taatatgtaa ctactttaat gtatttaatt tgttacttat accacttgtt
     3541 ataacttcat tctttgagtg tggagcagca gcagtaatag gagaatcaca aggtggtata
     3601 cgtggagcta taataggtac agctatagct gcaatattta tggtagtttt agtaggaata
     3661 tctgctgtaa tttactcagg aactattcaa aattggatac taatatttgg aggtaacgac
     3721 ctttcattat ttggtataat cagtaagttt ataggttcat tatttggagg aatattatag
     3781 atatgaaatt taaatacata gatgagataa gaagattaat tgatgtaata gaaaacgaag
     3841 aaaaagtcaa tatggaaaaa gcagtacaaa tccttgttga agcaatagaa agcaaaaatg
     3901 ctatattttc atttggagct tcacatgcag gaatattaac agaagaatta ttctatagag
     3961 caggtggctg tgttattata aatccagtat ttgcaaaatc tctaatgtta gatacagctc
     4021 ctataactca cactagcaag atggaaagat tagaaggata tggtacagct ctagctgata
     4081 agactccaat taaaaaagga gatgtagtta tagttcattc agtatctggt agaaatccag
     4141 taagtataga gtttgctatg aaagctaagg aaaataaggc aatagtaata tgtataacta
     4201 atttaagtta ctcaaaaagt gtatcttcaa gacattcttc aggaaaaaaa ctttatgaag
     4261 tatgcgattt agtgatagat aatcatggtc aaaagggaga tgcctgtgta gagatagacg
     4321 gattaaacca aaaggttagt ccaacttcta ctgtaatagc aactactgtg atgaactcta
     4381 ttatagctca agctactcag gaattagtta atagagggtt aaagaatcct ccgattttct
     4441 atagtgccaa tattgatggt ggagatgagt taaataagaa gatttttgat gagtataaag
     4501 atgtaattca ttatgaatac taaggtgaaa aaagtgtgtt aataaacttt gaagtttatc
     4561 aacggagaat attaataata tataataaat gctatcattt aaaagctgta ggtaaaaatc
     4621 tacagctttt tattattgaa tattaaaaag atgtgtaatt aaattttaaa ttggtgaaaa
     4681 taaattgaaa gatatcaatt ataaatatta gaaacgttgt catagaaaaa aactatatta
     4741 aaaaaaagta catagaaaaa actgattaga caataaatac atgctttgat aatattttaa
     4801 atggagtgaa aatgtgtaga attataatga aaaaagaaaa taaaaatgaa aaaagaaaat
     4861 aaaatggagg gaataaaagt gggtatgtta aaaaaattat gttcactagc tatggtagca
     4921 attatgacta caacacttat aacagggtgt tcaagtggaa atggaaaaga ttcaaaaaag
     4981 gatggcgaaa aagaaaaact agttctttgg atgccaccag tagaaaataa tatgaaggaa
     5041 gtttgggacc ctattttaga taaatttgag gaaaaaaata actgtgaagt agatttacag
     5101 ataattcctt gggataatta ttctgaaaag tttgcaacag ctataagtgc tggcgaaggt
     5161 ccagatgtag gatatatgta tgcagaaatg tatccacagt ttatagaaag tggagcagta
     5221 gaagatttga ctaactatgc aactaaagag gataaagagc aatctattta tataaaaaca
     5281 tctgaaatga tgggtggaat gtatggtatg ccattccaag cagcaaatcc aggggtatta
     5341 tactataaca aggatatttt agagaaatta ggagaaaaac ctcctaaaac atgggaagat
     5401 tttaaaagaa tttgtaaaaa agcaacacaa gacactgatg gtgatggaaa gatagaccaa
     5461 tggggattag ctcagggatg gggagctaaa acttttggta acctaaattg gaactggtat
     5521 ccttatttat ggcaagctgg tggagatatc tttaatgacg atttaaaatc agttaaattt
     5581 aatgataaga gtggtttaga agcagctaat ttcttaaaag aattacaagc atacgtacct
     5641 gaagattctc tatctaaaga tagtaatgaa atgatagaaa gtgtatttgg aacagggaaa
     5701 gcagcattta caataatgtt atcctctgct gcaacaagtg tatttgatga atcattccca
     5761 gatttaaatt ggggatttgt aactggacta gaagataaga aagcagcaac ttttggagca
     5821 gtagaccatt tatctttaat gtcatctgca aaaaataaag aacttgcata caagttaata
     5881 caacatatgt taagtgttga atcaatgaca gaatttcata aagcaatacc aagagcacca
     5941 ataacaaaag gtgaaccata tcaaggtgat gaaagattca aagaaatggt ggaaaatgac
     6001 aaagatgtat acagaccatt agtagtagga cctcatggag ttgaaatata tgaatattta
     6061 tggaaagaac ttcaaactat gatttcagga gataaaactc caaaacaagc gttagatgat
     6121 gctgcaaaat attcaaatga tttattagca caataaaagt ttaaagctgt aatatgttgt
     6181 aaagtacttt acagattaaa attagcattg tatttataaa tacaatgcta atatttatat
     6241 tggagaggat aaaatggaaa acttagttag agagtttgaa gaaaattcaa gaagaaaaaa
     6301 aacaaaatta aatattaaac catatatgta tatattgcca ttgggaataa ttttagtggc
     6361 gttttatgtg ataccaataa taatgtcaat atactttagt tttacaaagt ataatattat
     6421 tagtccagct acttttatag gtttagaaaa ttataaaaaa ttatttacag atgaaacatt
     6481 aaaagtatct attataaata ctattaaatt tactgttgtt gtagtacctt gccaaactat
     6541 attatcattg attttggcag tttggattac aggaaaagga aatagtaaaa tagcttcatt
     6601 tgctaaaggt gctatattta taccagtatt atcatctatg gttttgatag gtatggtatg
     6661 gagggcttta ttgaatggag aaggttctat tatatatcag gtattaggtg cctttggaat
     6721 agaatcatct aaacttttag gagatagtaa gacagcatta cctacactta tgtttatatc
     6781 tatgtggaaa aatatagggt atttcatggt aatttatatt tcagctataa tgaatttacc
     6841 aaaacactgc tatgaagtag cgaaggttga tggggctaca aaacttcaag aatttataaa
     6901 aataactgta cctttattga aaccaactac aataatggtt gtatttttag ggtcgatatg
     6961 gtcattacaa gtatttgact tagtttatac tgtgactggt ggaggtccag gtatatctac
     7021 tatgagtata gttatgcatg catttaactt aaactttaaa aactttaatt ctggatatgc
     7081 aatgactgta gcaaacgtat tatttttgtt aatagcggta gtttcgattt tacaaaataa
     7141 gcttgtaaaa cgagacaatt cagattttta gaaaggtagg tataatatga aaacagcaag
     7201 aagattatta agtgatggtt tactcttatt tatagcgtgt gcaagtttgg taccttttat
     7261 atacatgttg ataatatcat tgaagataac atataactca tatagtttgg atatatcttt
     7321 ttcaacagta acccttcaaa actatataga tatctttact aaaaaaggat ttgcacaata
     7381 ttttttaaat acagcaatag tatctttttc aggtgtacta ttaaatttag tatttagtac
     7441 attagcagga tattcttttg cgaagatgga ttttaaaggt agtgataagt tatttttatt
     7501 tatgataatg actttaatta taccttccca agttacaatg ataccactat atataataat
     7561 gaaacactta ggatggataa atagttatct tgcacttatt atgccaatac caactgcatt
     7621 tggcgtattt ataatgagac aagctatttt aggagtacca aaagagttat tagaatcagc
     7681 aaagatagat ggttgttcgg attttagaat tttaatacaa atagtattgc ctttaattaa
     7741 accagcatta attacactag caatatttac ttttatggga gcatggaacg agtttatgtg
     7801 gcctcttata gctacaacta aagatgcaat gagaacatta acagttggat tatctactct
     7861 taagacattc cagataacta attatggaca gatgatggca ggggctacaa taacattttt
     7921 acctccattt attttttatt tgattttaca gagtaaattt gttgaaggtg tgtctttgtc
     7981 tggtataaaa ggttaaatag tctaatttaa taatatataa agaaacttat ataaatatgg
     8041 aggaatttaa actatggaaa tttcatatgt actgaaagat tgcacattca aacacaataa
     8101 atactcagtt atagaagctg aaacttggaa aaataaggat ttgggtattg tagtagctaa
     8161 aggtgaaaaa tttgcatttc aaattatgct aaaagcaaca gaagaattta attgtacaat
     8221 tgataaaagc agtactatat catggaaagg tctgggaaat agagtaaggt tagcattaaa
     8281 tgtgcctaat agtttagaaa ataattttag tataaatata ttaggatatg ttcaagatga
     8341 tacaaaagca tttgtgaatg atgctatttt aagggataaa gatgtattag tagagcaata
     8401 tttgcctcaa acattttgga ttgaaggtca agtaccagaa gattttgaag aaaataacct
     8461 tgatataggt attgatatat ttaaaagctt tggatatgaa gatgaagaaa aagtttgtac
     8521 aattgatgtt cctgtaaaag taagaaatgt tgtgctaaag cctttagatg aaagcaagtt
     8581 ctttttagat ttatggcaac atccaagctg tttagctaga atgtataaag ttgagttatg
     8641 gtctgatata cattttgaaa tagtcgataa ctatttaaaa gaattagcat ctttaggaga
     8701 aaaagttgca acagttatag tttcagatta tccatgggca gggcaaagtt gttataaagt
     8761 ttataaaaat ccatcaaact tatatgaata taacatggta agtgtatcaa aaggattgga
     8821 tggaaagatt aaatgtaact ttgagagtat ggataggtat ataagcatag ctgataagta
     8881 taaaatggct caggagatag atttatttgg tctattaggt aattggtgtg ctggtgagtt
     8941 tggaaatcct gtagaaggtt ataaggaccc tataagagta agatattttg atgaaaatga
     9001 taaagtattt aaatttataa acaatacaaa tgatttaaaa gaatatatag gattattgtt
     9061 aaatcattta atagagtgtg gattgtggga cagagtaagg attatagctg atgaaccaaa
     9121 taatccagaa gtagtcaaag agtgtataga atttataaat tctactgtag gtactcatca
     9181 agttaaatat aaatcagcaa ctcacgatca aaacttttta gatagagcta aagatgaaat
     9241 tgatgatatg tcaataaatt taaaacttac tatacaaaat tataaagata tagaaagtct
     9301 taagaaaaaa atcaatgaca agggtgggat attgacttgg tttgtatgct gttttccaga
     9361 gaaaccaaat agttttttaa gttctccttt tgtagaaaat agaataatag gatggtatac
     9421 ttattacttt ggtcttgatg ggtttttaag atgggactat aatttatgga cagaagaccc
     9481 ttggaaagat tcaagttaca aattccctat ttggaaagct ggagatatgt tctttgttta
     9541 tcctggaaaa gatttaaagc cagttagaag tgtcagaatg gagaatttaa gatttggaat
     9601 acaggacttt gagctattta caatgttaga aaaagaaaag ggaagagagt atatagaagt
     9661 tgagcttatg caagaattat taaataaaaa agaaaatgct gaaattaaag gttttggtga
     9721 catagaacta ggatattcac ttgataattg tggatatgat aaagttaaaa aatatgtatt
     9781 agagttgtta gacagagtat aaaaaatata agggtgtctc aaaatgaatt ttttagttca
     9841 tgaggcactc tttttgtatg aaatcaaata tattttcatc gtttcaataa tgaaaaatag
     9901 gcttatttct attctgagaa agccctttag tttttagctg tatttagtgt tttaaaatca
     9961 actataaacg ctataaagtt tataaatgac aataattata tgtaacatga tgtactttaa
    10021 tttgctataa tagggattga ataatattat atagaagata acattgtttg gggggattaa
    10081 gataaaaaga ataggtatta ttataatgtt ttttttaagt atagctttta ttggatttta
    10141 tcttgtaata agagataaca gtgatacagt taagatgcaa cgaggaataa atataggtaa
    10201 tgcactagaa tcacctaagg attttccgtg ggatgtaaaa atgtcaaata agttttttga
    10261 tgatataaaa gatgcaggtt ttgatacagt acgtatacca gtaaggtttt ctgattatac
    10321 gtcagatttt gataatttta aaattgacga agaatttttt aaaaagattg ataaatatgt
    10381 agattatgca ttggataaag acttaatagt tgtgttggat ttacaccatt ttgaagaaat
    10441 tatgaaagaa ccaagagttc ataaagaaga gtttttaaaa atatggcaac agatagctaa
    10501 taggtatcaa aaatatgata aaaaattggt atttgaatta ttaaatgaac ctaaagaaaa
    10561 tttatcttca caattactaa atgaatacat tgaagaagca ataaagataa ttagaaaaac
    10621 taaccccaaa agaactatta tagtgggacc atataatttt tatcaaattg attatttgaa
    10681 tgaattaaat attcctaaag attctaatat agtagtttct tttcactatt atgaaccaaa
    10741 tgattttgca tttcaaggaa atatctatca taagggattt gaacacttaa gcaatattac
    10801 ttgggaagga acaaatgaac aaatggagta tctaaaaaaa aggtttgata ctgtggaaaa
    10861 ttgggcaaat aaaaataagg tgaaaatctt cttgggtgaa tttggaataa caaaagaagc
    10921 accagaagct tcaagaagag catggataaa agctgtaaga gaagaagctg aaaaacgaaa
    10981 cttttcatgg gcttattggg agcttgcttc tggatttggt atatacaatc aaattgaagg
    11041 aacttgggat agagatatat taagtgcatt aatagaaaag aggtgatttt atgaagaaaa
    11101 attcaaaaaa gatacttata actttaacta ttattactaa tataatatat atattatgga
    11161 gaatttttta tacagtacct aaagaagaag gtatgtttgc cttaatttgt gcaataatac
    11221 ttctttttgt agagataatg ggcatgatgg aaatgtttgt acattattat ggtatgtcga
    11281 atattgagta tcctgaaaaa ccaatcataa gtgaagaact ttatccacat gtagatgtat
    11341 tcattgcaac atataatgaa tctgttgatt tggtgagaaa aactgtaaat ggatgtattc
    11401 atatgcagta tccagataag aaaaaggtac atatatacat atgtgatgat ggaaatcgtg
    11461 aagaaatgcg tattctagca gaaaaaatgg gtgtaaatta cataactaga actgaaagag
    11521 aaggagcaaa agctgggaat ttaaataatg caatgcaaca tacaaattcc cctttgattg
    11581 ctacatttga tgcagatatg ataccaatgc atgacttttt aattgctaca gtaccatact
    11641 ttttgaaaaa tgagcaagcc aaaaaagatg gagaaaaaga ggagtatgaa aaggtcggtt
    11701 ttgtgcaaac tccacagagt ttttataatc cagacttgtt ccaatttaat ctgcactctg
    11761 agcgaaggat tccaaatgag caagattatt tttacagaga tattcaattg gctagaaata
    11821 ggacaaattc tgtaatttat ggtggtagta atacagttat ttcaagggaa gcacttgaag
    11881 aagttgatgg attttatact tattctatta cagaagattt tgcaacaggt atcttaattc
    11941 aaagtaaggg gtatagatgt tatgctattc cagaagtaca tgcatcaggt ttatctccta
    12001 cagatttaaa aagtcttatt aagcaacgtg aaagatgggc acgtggatgt atacaaactg
    12061 gtagacgtct aaatattttg tttaggagag gtttaggttt ttggcagaaa ataagttata
    12121 tttcttcaat tacttattgg tatgcaagta taaaacgttt tgtatatata atggcaccta
    12181 ttttattttc tgtgtttaat gtgattgtag taaaatgtac attacttcaa gtattggtat
    12241 tttggttgcc aatgtatatt ttaagtagtt tatctcttaa aatattttct caaaatataa
    12301 ggaatattag atggaccaat atatatgaaa ctataatgtt tcagtcctta atgccagcag
    12361 taatacttga aacatttgca atatctaaaa ataaattttc agtaacaaat aagagtaaat
    12421 tagaagaaaa tagaatgtat aagtttttac agggaatacc ttattttatt tacatggtct
    12481 tatctatcat aggaatttta aaaatgtttg tagcaatatt taaaatgagt tcaataacat
    12541 attcagttgt attattttgg ttgattggaa accttttcaa tcttgttatg gctacactat
    12601 ttatatcagg aagacaacag ttaagaaagt ctgagcgtta tattgctgaa attgatttta
    12661 agttaaagca aaattcttat gttctttctt caaaaacgat tgatatatca gaaaatgggt
    12721 ttgcgttttt gctagaaaat ccagaatata tttccccaga agaagagttt gaggtagagt
    12781 ttagagaaaa gtctggaaat gaaatgtata tagctaatat gaaagcaaag attgttaatg
    12841 ttgtggaagt aaattcaaag tggaaatatg cagcttatat tacacatatt gaagatagtg
    12901 aaatagataa ttggatgtgt attgttcacg atagaatacc tactttaccg atgacaatat
    12961 ctaatcaact aggtttcttt gatgatttgc aaattaatgt aaaaaaacgt attgaaaaaa
    13021 ctagaacatt atcacgaaga agtccaagaa tcaatatgaa ttttcagatg gatataaaaa
    13081 atacaggtaa attaagaata gtaaatttta attatcaata tgtattatta aattttgaga
    13141 ataaaaatat ttatcctaaa gaaatagaat tggaaattaa tgaaggtatt gtattagaat
    13201 gcgatttatg tgagggtaaa attgatgaaa gaggtatctt atatagagta aataacattg
    13261 acagcataat gcaaaatttc tttttaagag atgaaatgat ggattggata ttacaaaata
    13321 agacaatttt tgtttcaaag cctagtgaaa agaaggaaaa aagtatagat gagtttgaac
    13381 ccatggaata tatttaggag agatgaaatg aagactttaa aagtgttttt ttgttctgca
    13441 aaatatctta ttttggtgtt tatttttttt ataaatacta cttttataag ttcagcaaca
    13501 agtgataaaa ttttagattt atcctttaag aagatagaga ctgacttaag ttctaagatt
    13561 acatatgaag atacaggtgt aaaaatagaa acggacagtt ctaaaagtga taaggaacgt
    13621 tatttatata tttaccaaaa tataaaagaa aattggagta tgtataataa tttttatatt
    13681 gaaattcaaa ataaaaataa aagttcccag agaataaatt taagtgtaca aagtaagaat
    13741 atgcttgaat ttaggttaaa agaaggtagt gaaatatttt tggaagataa aaatatagta
    13801 tattcagata aaataaaaga aggatgtata gaagtaccag gtgaatttga aggcaaaatt
    13861 tatgtgaact ttaatagcct tataaatgag gaaagtaatg ttgtgctgga ctcaaatatg
    13921 ctttcaaaca ttgttagttg gggaattacg tttattccat ctgatgaaga acataatatt
    13981 gttattataa aaaagatatc tttattgtct aaagaaaaat tagtattttt aaataatatt
    14041 aaaataattg gtgatgaaga agtacaaata ccagtattgg gtcaaagtat aagtcagtat
    14101 gaggtattag gtcttaaaag tgattctaaa attaagtact ctcttatggg aaaacaagat
    14161 aatgtgagca tatctcaaaa cggtaaatta acattaagta ataaagccaa acctggaaaa
    14221 attattttac aagttaatgt tgatgataag tttaaaattg gaaagaaaat aacattaact
    14281 gagtcttgga gtgttaataa aaaagataag gatggtgttc catatacatt ggtatctcct
    14341 gaacaatcac caacagtaca agatatgaaa aaaataaact ttatgaataa tatcataacg
    14401 tttgttcgaa ttttatttgt atctttagta ataatatgct ttggaatata tttatattgg
    14461 aaaaaatgtt caaaaactaa gtaaagagag gaggttagat tgtgttattt tcaagtttaa
    14521 tttttttatt ttacttttta cctataacat tggttttata ttatgtattt aggtttaatc
    14581 gaacgataca gaacatgata ttgcttgctg ttagcttatt tttttatgct tggggagagc
    14641 caaaatttgt tgttatcatg atagtttcca taataatgaa ctatatattt ggtttactgg
    14701 tagatagata tagggaaagt aaaataaaag taaaaatttt tttagttttg atgtgtgtat
    14761 ataatatagg tgttttattt atatttaaat atctagcttt tgcgctaaga aatattagtg
    14821 aacttgttaa tattgaacta actataccta atattatttt acctatagga atttcatttt
    14881 ttacatttca aggaatgtct tatgttatag atgtatatcg tagacatgga gaagtccaaa
    14941 agaatccttt ttatgtggga ctgtatatag catttttccc acaattaata gcaggtccta
    15001 tagttagata tgagagtgtg gcagaacaaa ttctcaatag aaaagaaact tggaataaat
    15061 tttctattgg gacttgtagg tttataacag gtctaggtaa aaaagtatta atttcaaata
    15121 acatggctat agtagcagac tatatttaca ctatgaatag tcaaggagaa atagctgcat
    15181 cacttgcatg gcttggttca atagcctata ctatgcaaat attctttgat ttttctgctt
    15241 attcagatat ggctataggc ttaggtctta tgtttggttt taaatttgaa gagaacttta
    15301 attatccata tatatcaaaa tcaattagtg agttttggag aagatggcac atttctttgg
    15361 gaatgtggtt taagagctat atatactttc cgcttggagg ttctagagta gcaaataaag
    15421 atattatgat tagaaatatg tttatagtgt ggttatttac tggaatatgg catggagcag
    15481 aatggacatt tgtaatttgg gggattttaa actttgtttt cctaattata gagcgattta
    15541 taatgtttga aaagattgaa aatcacaatt ttataaaaca tatttatgct ttattagtag
    15601 ttaattttgg gtgggtatta tttaggtcac ctaatttaaa agaagcatat aattatttta
    15661 aagctatgct tggaggcaat ggggttatat ggagtgatta tacatatatg tttttaaaag
    15721 aatactgggt tttctttata tttgcattta tttttagtgt gcctattgca aagaaaatca
    15781 ataagtttgt tgttgaacaa gttaaatata gtatgatatt taatgtattt tatccaatct
    15841 cagctatttt attgttcttt atttctgtga catatttagt aactggaagc tataatccat
    15901 ttatttattt taatttttag ctgaggagga aaagtatgaa tagatttttt aaagtaagat
    15961 taataactgc aattatgttc ttaattgttt tattcgcttt ctctatattt aatatttgtt
    16021 attctggaaa agatatgata caggagataa aaaatttagt tgctgaaaaa agtagtttaa
    16081 aagatacaat atcatctatt gataacacag taaatgacaa agtaatatat aaaaatacgt
    16141 tcatagaaac ttatggctac ttacaaaagt taatgggtaa aaatgagttt tcaaaattta
    16201 gttctgtaaa agatacttct ggaaagatgc attatacata ttttgctaaa gctccaaatc
    16261 caattggtgt tatatcagat agagtaagaa aattttctga agaaatggaa aagcaaaaca
    16321 ctaaattggt ttacctaatg actccagata agtatataca aggagtgaca aaatttccaa
    16381 aagggatacc ttattcctat agcaatgaga cagcagataa ttttttaaat caactaaaaa
    16441 agtataatgt ggactatatt gattttagag aaaatatttt aaagagtggt attccaaaag
    16501 atgatttatt ttttaaaaca gaccatcact ggaaagtaga aacttctttt tgggcatttg
    16561 gggaattagt tgagcaactt aataaaaaat acaatatgaa tttagatgaa aatcattatt
    16621 atagagataa agaaaattat aattcaattt tatatcctaa gtcatttctt ggttctatgg
    16681 gaagaaagtc tggtatttta tatggtggta tagatgattt tacattaatt tatcctaaat
    16741 ttaagactaa ttatacttat tatacagatt ctaaatttca aaaatttgaa ttaaaaggaa
    16801 ggtttgaaga gagtttggta ttatcatatc cctttaatgc agaccttaat ttaatggatg
    16861 gtcaatcaga taaatattct acatatttat tggggaatag accactagtt aaaataaaaa
    16921 atatagaaaa tccagatgga ttaaaagtgc tatttgttaa ggattctcta attgtaccga
    16981 cagcagcatt tttttcctct gtttgttcat ctatagatat gatagaccct aggtattatg
    17041 atggagatat actagaatat gcaaaatctc atgactatga ctttgttttt atatctatat
    17101 atccccaaaa tcttacaaaa gaattttttc cgttttgtga gtaaatttct aaagaaattt
    17161 tcttataaaa taaaaaacaa atggctaact ggttaaaggt tagccattta ttttaagagt
    17221 tattctaaga aatatttttt ttactaaata cttctttatc tatttttcca agtgattttc
    17281 ctacagtaat tgatgatacc atagcaccat taacatttat catagtacgt cccatatcta
    17341 atatagggtc tattgcaagt ataccaccaa ctaatggaaa ataagaacca agaccaactc
    17401 cagagataac aactgaaacg gccattgtag cagttcctgg aagaccagct attccaaacg
    17461 aactaattgt tataacaatt aacaacataa cataaaagct catatccatg tttactccag
    17521 ccatattggc aagtgttaca gccattaaag caggatatat acctgcacaa ccattcatac
    17581 ccatatttgc acctaaactt gctgtaaagc ttgatacacc ctcatcaact ccgtgatttt
    17641 gtactagact ttctatcgtt acaggcaatg ttcctaaact agaacgtgaa gtaaatgcca
    17701 gaagtaaagc ttcagttgaa ttttttatat aagtaatggg atttagacca tttaaagtta
    17761 taaaaattaa atgtatgata aataatattg caactgatat ataaagagct aatataaact
    17821 gtaaaacaga tactattgaa ctaatacctt gtgaagttat tgtatttgca agcaaagcaa
    17881 ccacagcata aggcataaat ttaataacag tcatagacac acttacaatt attttgtaag
    17941 atgcttctat aaaatctata aatggtttta ttgtttcaaa atgtttttta cttaatctct
    18001 ttattgaggt agctataaac gtagcaaata ttattgtagc tacaatattt ccatttgcca
    18061 tagccattac aggattagat ggtagtaatc ctcttaaagt atccacaaga gagtttattt
    18121 ctttaagttc ttgattttca actaaagtag gcatattagc tcctacacct aacttcatta
    18181 tatttgcaac gataattcca acaattgcag ctatagcagt agtagcaaga agcataccta
    18241 aagatttaaa tgtaagagtt ccaagattgt tatctttcat atttataatt acttttataa
    18301 tggataagaa aactaaaggt actactaaca tttttaataa gtccataaat ccagaaccaa
    18361 ataggctata ccacttgctt acttcttgta gccaagtgac ttgagtggta tcattaggaa
    18421 aaccagcaaa agcttgtatt atgactccca atataagtcc tagtactgta gcatatattg
    18481 ttctcaatga aaattttatt tttttctttt caaattgtct aacaataaag aatgtaccta
    18541 gaagtaatat tatgaaaaaa atcgttttaa aatctgtaat cataagaaat tgtgataaaa
    18601 atgaactttc caatgttttt tctcctttta tttaaaaata aatttttcta agtttaaatg
    18661 atatcaatat attaaaaaaa tgtcaatgtt atatcacaat ttacttaaat agagtaaaac
    18721 aagttgattt tgaaaaatgt gattaagtat agaaaaatag ggtataataa atgtttaaag
    18781 aatttgaatt gtggtaagaa aataaaaaat agcttttata agatttgatt tcaaattaaa
    18841 atatgttaaa taaataacta aaaattgtta tgaaaaatgt ttacatagaa tatatatcaa
    18901 atgaaaagct taaaataagt gaagaaatta actagacatc aaaggagaga taaaatgaga
    18961 gtagtaataa ttggtggtgt tgcagctggt atgagtgctg cagcaaaatt aaaaagaata
    19021 aaacctgagt atgaagttgt tgtttatgaa aaaactgaaa tagtatcttt tggtgcttgt
    19081 gggttgccat actttgttgg tggatttttt gatgatgctg atgaattatt agcaagaaca
    19141 ccagagaaac ttagagaagc tggaattgat ttaaatatat ttagagaagt cgttgaggtt
    19201 gatagtgaat ctaagaaaat aaaagtaaaa aatataaaaa cagatgaaat atatgaagat
    19261 tactatgata aattgatgat agcaactgga gcaagaagta ttatgcctcc tataaaaaac
    19321 ataaaattga agaatgtttc tacattaaaa agtttatatg atggagaata tctaaaaaaa
    19381 cttttaagta atgaagataa taaaagagtg acaataatag gagctggttt tatagggtta
    19441 gaagcagttg aggcatgtaa aaaattaggt aaagatgtag atgtaattca gttagaagat
    19501 agaatattgc cacaagtatt tgataaggag attacggatg tattagaaga agaaataaat
    19561 agacataatg ttaatttgca cttggatgaa atggttgtag aactttatgg agaagataaa
    19621 gtagaaaaag ttataacaaa caaaggggaa atagatacag atgtagtaat aattgctact
    19681 ggtgtgagac ctaataccga gtttttgtca aatactaata taaaaatgct aaaaaatgga
    19741 gctatagtag ttgatgagta tggaagaact tctgtagagg atatatactc tgctggagat
    19801 tgtgcaacta taaagaatat agtaagtaat gaaaatgtat atgttccttt agctactgga
    19861 gctaataaat taggtagaat agtaggagaa aacctagcag gaagagaagt atcttatcaa
    19921 ggttcactat cttcaagttg tataaaaatt atggatatgg aggcagcatc aactggaatt
    19981 acagaaagac aagctaaaga tttaggcata aatatcaaat ctaaatttat ttctgattat
    20041 aatcaaactc attattatcc aggaagaaat aaaatatatg taaagcttat ttatgatgca
    20101 gatacaaaag ttattttagg tggacaagtg gctgggttta aggatgctgt ccaaagagca
    20161 aatgtattgg cagctgctat ctttggaaaa atgaccacaa gtcaacttgg aatgttagat
    20221 ttatgttatg ctccaccatt tgcaagaaca tgggatgttt taaatgtagc tggaaatgta
    20281 tctaaataat atttagatta tagctaatat agtttacaag caattaaggg aatgaattta
    20341 cctaaaatat tcttatcatg gtaaaattca ttcctaaatt tttattccaa atttttagaa
    20401 ataaggattc tattaaatta ttcatcaaaa tttaaacgca agcttaatct atcctctcca
    20461 agaatagtat tttcaaaaac attagtagca ttttctaaat acatactagg tatgtctaaa
    20521 gaaggactaa aatgagtcag taaaagttgt ttgacatttc caagttttgc aagattagca
    20581 gcttctctaa aagtcatatg tttgtttcta acagcttttg atatatctaa atcatcacca
    20641 tacatggatt cacatataaa taaatcacta tcttttatga aatctggtat tgattctata
    20701 ggtcttgtat cggttgtaag acttatctta actccctttc tcaattctcc taagaccata
    20761 tctggagtgt attgttttga atctaaaact atattttgac cttcttggag tttgttccat
    20821 aatattttag gaacttcgtt tttagttgcc ttatctatat caaatttagg gtttcttcta
    20881 aaattaaaac tgtatcctaa acactctata gagtgttcta aactgattgt tgaaatttct
    20941 aaatttctta aaactttatt atctaaagaa aaattgcctt gagggttttc tataattttt
    21001 aaagtgtatg gcacatattc tactaaattt ctcatagaac gtacacaatc gactattcca
    21061 actggaccaa ctatagttaa atcacttgta cgaccactat ttccaattgt tgataaaagt
    21121 cctaaaagtc cgaatatatg gtcaccgtgt aaatgggtta tacaaatcaa gtctatatct
    21181 ttaaagccac atttttttag cttcatagat acctgagtac cttcaccaca gtcaattagt
    21241 atttttctac ctttatagtt tataaataca gaggatagaa atctgtttgg cataggtaca
    21301 tttccaccac atccaagtaa agttaaatct atcatttttt cccctttcta aaattaacac
    21361 aaatctgttg tttgttttta ttatgatatc ctattataat tattttaaca ttaaatataa
    21421 caataaatgt gtatttgagt caagaaatat tgtcaaataa agtttttata taacaatata
    21481 taaaaatttg gagaacgaaa agtgaaaatt atatttagga gtatttaaag gaggaaaatt
    21541 taatgaaagt attaaagaaa attactattt tgtcattgat tacattaact ttagttggtt
    21601 gtagtaacgg agaagagaaa aaagtggttg aaggttctaa gaaaactcaa aattctaaaa
    21661 aagatgaaca gcctataata gtacataaag atgcaaatat attgaaagat aggtatgagc
    21721 caccaaaagg atttaaaaga gttgatgtag aaaaaggcag ttttgaatca tttcttagga
    21781 acactaagct aaaaaattat ggagagaaag taaagtatta tgatggaaga actaaggaat
    21841 caaaaggtat ttatgatagt gtatttgatg ttgatattgg caatagagat ttgcatcaat
    21901 gtgcagatgc aattatgctt atgagagcag aatacttgta tcagaataaa ctatatgatg
    21961 aaataagttt tgattttgtt gatggcttca aggcgaagta ttcaaaatgg gcacaaggat
    22021 atagaatttc agtaaaagat agtggttcaa gttggtataa agcaactgaa tattcaacat
    22081 cttatgagag tttcagaaag tttatggata tagtgtttgc atattcaggg acgttatctc
    22141 ttgaaaaaga attagagcct gtaaaagttg aggatatgaa aataggagat gtgtttatag
    22201 ttggaggaag tccaggacat gcaagtataa ttatggatat ggcagaaaat gaaaagactg
    22261 gtgaaaaggt atttatgata gcccaaagct atatgcctgc acaacaaacg caacttctta
    22321 ttaatcgtaa aaatccaaat ttgagtcctt ggtatagttt agattttgaa ggtgatttga
    22381 caatacctca atggactttt aaaagagacc agttgaaaag attctaaaat aataatttta
    22441 tataataaaa gaaaaatata gatgtgaaaa aatgtaatta gattaaaact aattggatat
    22501 ggacaaaatt aaattgatag ataaatatta aaaattacac taattaatct tactatagtt
    22561 taatatttat ctaaaaagtt ttatgtaaaa tataaatttt atattaaagc taaaactaat
    22621 ttgaggaggc taatttatat tgaagattag aaggtattta attttggtaa ttataattct
    22681 aattacaaca agtcagagtt ttatttctta tgggctagat cgatttgaag aaataactat
    22741 ttttcacact aatgatgtac atggaagata cgctgaaggt gatgaccata tacaaatggg
    22801 aaatctagcc acattgaaaa aagaaactcc aaactcaatt ttagtagatg caggagactg
    22861 tttgcatgga cttccaatag taaatatgga tagagggaaa agtgcaattg aattaattaa
    22921 gtgtgctgga tatgactata ttactcctgg aaatcatgac tttaattatg gaaaagatag
    22981 attgtttgag ctatctaaaa gtgctaattt aggagagaat actttaaaat ttttatcatc
    23041 aaatattttt gaaaatggga aaaaagtttt tgatagtaat gatataaaag aaatagatgg
    23101 aataaaaata ggattttttg gactctctac ccaggaaaca aagagcaaaa ctggatttaa
    23161 aaatgtggaa gggttagagt ttaaaaatcc tatagaaagt gcaacagagc aagtaagtga
    23221 attaaaagga aaaggtgcca atgtaatagt tgcaatatgt catattggta caaacagttt
    23281 aagtaagcca aatagtatag atatagcaaa tgaggtccaa ggaattgatt tgattattga
    23341 tggacacagc cacactaagt ttgaaaatgg taaacaagtg ggtgatacat tgattgtaag
    23401 tgcaggacaa tatttagaaa atattggcga gataaaacta actttggata ctaatgatgt
    23461 agaaaagata aaaatagaaa ataaatcagc tagattaatt ggaaaagaag aagcattaaa
    23521 gtataagctt gactccgttg taagtaaaaa ggttgatgaa ataaaagaac aacaagaaaa
    23581 aatattagat aaagttatag gtgtaactac aaatactcta gatggttcat atgaaaatgt
    23641 aaggacaaaa gaaactaatt taggtaattt aataagtgac attttactag ataaaactaa
    23701 ggctgatatt tctttattta atggagggaa tattcgagat acgatagaaa aaggcaatat
    23761 aacaagaaga gatattgtag atgtctttcc ttttagcaat actatagtta ccaaagagct
    23821 tacaggagca cagattaaaa atgtattgga gcatggagta aaactttatc cagaaaaaag
    23881 ttcagccttt ttgcaagtag gaggaatttc atattacttt aatcctaaac aaaaagaagg
    23941 tgaaagaata acaagtattc aaaaagaagg aaagccatta gatttaaata aaaagtatgt
    24001 agttgcaacg aatgattata tagcctcagg tggagatgaa tttccttgtt tttcaaaaga
    24061 gccagtacta aaggagtttg gaaatttaga gagtacagtc attgaatata tagagtacaa
    24121 aaaagaaata tcaaaaaatg tagatggaag aattggtatt aaatctaaag atgagttaat
    24181 aagaaagaat catgagagtg atttgatatt aagtgaaaat gaaaaaaata tcaatgataa
    24241 tgaagagaat ctatctaata atataaaaag ctctgatagt gataaaaata aaagcttaga
    24301 taaaacaagt gctagagaaa gttctaatct aggaatatct aaaagagaag ctaaagaaaa
    24361 atctcctaaa acaggagatt taggtttttc aaatagtata attatatttt tagtttcaag
    24421 tacattaata tgtttattga attttaatca aaaagagtta aaggataaaa agtcaaaata
    24481 aaaattaatg agcaaaaatt agtagatagt aatttaattt tagataatag caaacagcaa
    24541 aggaaattag ttatataaat actaatttcc tttgttttat actttaacat gattactttt
    24601 aaataaaatt gtattttata aaaaataatt acactaaatg gtatagtatt atgggtattt
    24661 ttagttttta ttcaagtaaa tgaaatcata ttaacaactt ctgatatatt tacaaattga
    24721 taaaaaaatg attttattag tatgatataa tttagctagt atgtatgata atggaggata
    24781 tagggatgaa taatatatta cttttggaag atgacaaatc tttaaataga ggaattagtt
    24841 ttaaattaaa aaaagaagga tacaatgttt ttagtgcatt tggtatagaa gaagcaaaaa
    24901 atattttcat aaaagaagag atatgcttaa ttatttccga tataggtcta cctgatggta
    24961 gtggttttga cttttgtgag gagattagaa agaaaagtga tgtttacata ataatgttga
    25021 cagctcttga tgaagaggtg gatattgtca caggatatga tttaggcgca gatgattata
    25081 tcacaaagcc ttttagtttg atggtattaa tatcaaaagt gaatgcgctt atgaaaaggg
    25141 taaatactgt aaaaaattat actctattgg tttgtgatga tttatttttt cactatatag
    25201 aaaataaatt gattataaaa acagatgata aagaagaaga gataatttta agtaaaacag
    25261 agactaaact cttaaaatat ttgatggaaa attctatgca gacattgact aaagagcaac
    25321 tgttagaatc tttatgggat aatagtggta attttgtaga tgataatacg attgcagtaa
    25381 atataaggag acttagacaa aaagttgaaa aaaatccatc tgaacctaag tatataaaaa
    25441 cagtaagagg ggttggatat atgtggggag aaaggagtat aaaaaaatgc taaaaaatat
    25501 gaacctcaaa gatggtttaa gtgtgaaaga tgattatata atgagaagag ttattggata
    25561 ttctatttta tctgtactaa tagtaaccct tgtatttata ctttttgaat atagtagatt
    25621 acataaattg tatgaatatc aaataaaaaa tactcaaaat attgctggaa tattaattga
    25681 aaaatatcct aatgatgaaa tagatatagt aaaatctatt tataattctg attatagtaa
    25741 ggtgtctatt ggagaaaagg catttaagaa atttggctat ggattggaaa ataaaatgtc
    25801 aaatgataaa aattttagtt tatacttaaa atcttttttt aaggaatcat tttttatttt
    25861 tttaatgatg tttatagtag ttatagatat aatatattat tttataagat acataaataa
    25921 aagattgagt aaaatttatt ttatagtaga taagatggca caagaaaact acttgaagga
    25981 cactactgag tcagaagaga ctagttatct agtagaatat aatgagtatt ttaaagaagg
    26041 tacatttagt aagataaata actgtctata tgagcttaat agaagtttaa agataaagtt
    26101 tattaaatta gaaaaagaaa aagaaagtgt aaaatcatta gtaacagata tttcacatca
    26161 attaaaaact cctttagcat ccttaaaatt atacaataca ttacttatag aagaagaatt
    26221 agaagaagat gaagccaatg agttcttatt aaccaataag gcttctataa ataaattaga
    26281 aaatttaata aattctttag tgaacatatc aaggttagaa attagcatga taaacataaa
    26341 aaaagaagat aatgatataa aaaatactat tctaaatgca gtaaaaagtg ttacccctaa
    26401 ggctaagagc aaaagtataa cattaaattt aaatgaattt aacagtagaa ttataccaca
    26461 tgataaaaag tggactgagg agagtatttt taatgtatta gacaacgctg taaagtacac
    26521 acagataaat ggagaaataa atatatctgt agaagaaact gctaattact ttaaaattat
    26581 aatagaggat aatggcatag gaattgaaaa aaatgagttt aataatatat ttaaacgttt
    26641 ttatagaggt tctgctgatg aaattgaatc tgtagaaggt agtggggtag gattgtattt
    26701 aagtagaaga attttagaag agcaaggtgg aaatattata gtttcttcaa aagtagggat
    26761 agggagcaag ttttctttgt ttcttacaac tatgtaatat taaatgaaag gaaaatgtaa
    26821 gcctctacat ttattatata agtataaaca gctccagaaa taaaaaatta ataaaatatg
    26881 gggtaatata acttagaatg agaggataat tttatgaaaa tactatatac tgaaaattta
    26941 tcaaagcatt atggaaaagg agaaagctta gtaaaagctt tggataatgt taacttagaa
    27001 ataaatgaag gtgaatttgt tgcaataata ggaaaatcag gttctggaaa aagtacactt
    27061 ttgcatatga ttggaggtct tgatatacct acatctggca aggtgtatat agataataaa
    27121 aatatattta cattaaaaga agaagaactt gctgtgttta gaagaagaaa aatagggttt
    27181 atatttcaaa gctataattt aattccatct cttaatgtat gggaaaatgt agtgttgcca
    27241 ataggtctag atggtagaga ggtagatgaa tcttttataa aagaactttt gaaatcatta
    27301 gggcttgaaa ataaacacga cgttttacca aatacattat caggtggtca acaacaaagg
    27361 gtggcaatag caagagcatt ggctacaaga ccagctataa tacttgctga tgaaccaact
    27421 ggaaacctag attctaaaac atctgatgag gttatgagta tattaaaatc tatgtctaag
    27481 aagtacagtc aaactctagt tatgataact catgatgaca gtatagccca aatggctgat
    27541 agagttatat tcatagaaga tggcagggtg tcaaaggttg gtgataaaaa tgattaatac
    27601 tactaaaata gcaaaaagta atttaaaaca aaataaatca aaaagtattc taataataat
    27661 tactataata ctttcaacct taatgctttc atctattgga atttatattg ttgatgcagg
    27721 ggcatatcaa aaagaaaata ctataaagta ttcaggcaat tatcaaggta tattagcaaa
    27781 tgtagacgaa aaacaagctg atatattgag taatcatgca gatgttgaat taactggtaa
    27841 aatgaatgga gtaggtgttg aaaaactaga agatgattca aatatttctt tagcatacat
    27901 gaataaagat gctttgaaat taaatagttt tgaatttgtc aaaggaaaat tgccaataag
    27961 agagaatgaa attgtattgg atagtggggc tttaaaagca cttggttata aaaataaaga
    28021 tttaggtgaa aagataaaaa tatcttataa tgactataaa aatgataaaa aaattgaaaa
    28081 agagttcatt ataagtggaa ttttaaaaac tagcgaaata tctgaagcag gtaaatatta
    28141 ttatgctatt atatcagaaa catatatgag aaatactaga gatatgtcac aagaagattt
    28201 taatatatat gtgaaattta atgataaaag taatttatca atagaacaag taaaagaaaa
    28261 attaaataag attgctaatg atataggact agatactatt aatactgctg taaatgaaaa
    28321 ttatataaat gctttaaaac cagatatgga aacgattatg ggtggagttt ttgtaggatt
    28381 agtaattgta ttatctagta ttttagtaat atacaatata ttttatattt caatagttac
    28441 gaaagttcaa gaatttggaa aacttagagc aattggtgca actaagaaac aaataaaaaa
    28501 tatagtgttt aaagaagggt ttatattagc cggaatagca ataccaatag gaataatttt
    28561 aggttatgta cttgcaaata ttataataaa attatttatg gatatagatg taaaatcatc
    28621 acgattacca gttatattat cggttgtggt aataagcttt atatctgttg ttttatcact
    28681 tttaaaacca atgaaagtag catcaaaagt atctattgtt gatgcagtaa gatatacagg
    28741 aaataaaata agtaataaga ataagagaaa aggatataaa aatataaatc taaagagatt
    28801 atcacatgcc aatttagaaa gaaataaaaa acgtacttac atgacattgg catcacttat
    28861 acttagtgga actatattta taacagtatc aactgcttta gaaagttttg atgctgagaa
    28921 aatggcaaga gaacactttc cttatgatat agaagttaga ctatctggtt atgaaatgaa
    28981 cagtgataaa aatcctaaaa ataatttgaa tattttacaa atggataatc ctctaagtaa
    29041 agattttttt aatcaaataa agaacatgga aggaattaaa agaatagaat ctgcaagaag
    29101 tgtaaaaatt ggtatggagg actatgatgt tgaatttaag tatgaccttt tacaaagtat
    29161 aaatgaaaat gatgtaaaat cattaaacaa gaatctaata gatggaaaaa taaatcttga
    29221 aagactgcaa actggagatg aaatagtaat tactcatgtt gatacagcaa aagaaatggg
    29281 agtaaaagca ggagataaaa taagattaac cttatatgat ggagataaaa agataaaaaa
    29341 agaatttaaa gttcaagcaa tagctatggg ggttccaagt tttggtatag ggaaagattt
    29401 tatagataga actttgaaat acgattcaac tagtagcttg ggaatatata cagaagaagg
    29461 gaagtatcaa gaaatcaaag atagtatcaa gaagattgct aaaaataatg gatttttaga
    29521 gactgatttt atagatagta gaattgagtc aaataaggct actatttcat ttataaagat
    29581 aatgggatac acgctaacag gcataatagg tgtgattgga tttatgaatc ttgtcaacac
    29641 aatgataact agcatagtta ctagaaaaaa agaacttggc atgcttcaag ctattggtct
    29701 tactaataaa caattagtga aaatgcttaa ttcagaggca gtgtattaca cttctggaat
    29761 gatgatagga agtattttat ttggaggtat attagggtat attgctgtta tacttctaaa
    29821 aaagactgga ttatcttatg caacatattc tttgccaata gtaccaatct tgttgatgat
    29881 tgtgtgtatt cttatagctc aatttataac aacttatttg ataggacgaa gttttaataa
    29941 agaatctctt attgatagag taagatacag tgaataaaat gcaaatattt atggttagtt
    30001 taactagcaa aaagcaaata aaaaatatag ttaaagagat tcataattaa ataggttaaa
    30061 gtatagtcac agagtaaaca atttaaactg atgtttgctc tgtattttat tatgtaatca
    30121 ataatatatc taatagactg atattattca tgttggaaaa tgtatcggta caattctaaa
    30181 aaagtataaa attgtaccat ataaaatgaa aattttgtat aataatcgta tagaaaataa
    30241 ataggagctg atatatatga ataagaagat taattatgca ccaggaccaa ctgaaactag
    30301 ggaaaatgta agattagcaa gagcagaaaa aacaacaaat ccagatatag atattgattt
    30361 tgttgaattt tataaaaaga catgcgagaa gtttggcaat ataattggaa caaaaaacga
    30421 tgtttacata ttgagtggtg aaggaatatt agggcttgaa gcagcatgtg catcacttac
    30481 agaaaaggga gatagagtac ttgtaattga caatggtata tatggagaag gttttaaaga
    30541 ttttgtaaca atgtatggtg gggagtatgt gcttttttca agtgaatata ctaaaagtat
    30601 agatattgat gaattgaaag catttttaga taaggatagt aattttaaat atgcaacagt
    30661 agtgcattgt gatactccaa ctggtgtatt aaatgatgta agcaaaatat gtccattact
    30721 taaggaatat ggaatattaa ctgtagttga ttctgtagcg ggtatggtag gagaacgatt
    30781 aagtgtagat gaatctaaaa tagatataat attaggtggt tcacaaaaag ctatttctgc
    30841 accagcagga cttactatag ttggaataag tcaagatgca aaaaattgta taaaaaatag
    30901 aaaaactgat gtaataggtt tttattgtaa tttaaatata tgggaaggct attatgaaaa
    30961 aaaatacttc ccttacacaa tgccaataag tgatataatg gggcttgata aagcacttga
    31021 taatatatta gaagaaggaa tagaaaatgt attaaataga catgaaaaga tagcttatag
    31081 tgttagaaaa tctattgagg aatatggtct agaactattt ttagaagaag gatattctaa
    31141 tacagtcaca gctataaaag ttcctgaaag tataggagct ttaaaactta ctgattatat
    31201 gcttaaaaat tataatacac ttgttgcgac atcactaaat caatatatgg atactatttt
    31261 aagaataggt catatgggag aaaatgcaaa tctaaataaa atagagcaca tactaaatgt
    31321 tttggataaa agtttaagtg cgttaggatt taaaggaaat ggaaatttgt taaacttatt
    31381 taataagtat tatttttaat agatagatag ggggatatag tatggaagtt acagctaagc
    31441 aaaaatttac aacgaaagac atggtggaaa cctcactatt aatagcatta gtatttattg
    31501 caactaaatt tataaatata agattaccaa tatctataaa tggtgggctt gtgcatttag
    31561 gaactgcaat gttattcata tcagcaatag tatttggaag taaaaaaggt gctttatcag
    31621 gagctattgg tatgtcactt tttgatttaa tatctgggtg gactttatgg gcaccattta
    31681 cctttatagt tagaggtgtt atgggttatt tacttggaaa gattgtatgg gcgaatggta
    31741 ataatgggaa taatttttta atcaatgtaa taggaatatg tgtttcatca atttggatgt
    31801 tatttggata ttatgtaaca gaagttatac tttatggtaa ttttgtagta cctcttactt
    31861 caataccagg taacttaatg caagtattga taggtcttat aatagcgctt cctatttcta
    31921 aggtattaag aaaatgtata aaataaatat aaagtgaact aaaatttacg atagcataaa
    31981 ttatactgta taatattaag atggagtaca taattaaaaa attttagtat agaggttagt
    32041 tcacaatgtt atcagttaca agatattcaa tcaaaaatat tcgtttaaag ccgtgggtga
    32101 agtttatttg gtattttgag acaaaatcaa atatactgtt aaataataag ttacttccga
    32161 cagacagtat agatattatt cttaatcttt ctgatgttat ggaatataaa attgaaaatc
    32221 aagactatac tgctagtaat atgcatttta atggaataag ggataaacat ggatttatta
    32281 ttcaatatgg aaatatacgt gttataggga tttcttttta tccatttggt ctatatccat
    32341 ttttaaagat accgatttca gagtttaaca gacaaattgt tgacctagaa gcaatatcaa
    32401 aactatttgc aaaaaaatta gaagagtcat taaatccaac aaaagcaata gaaaaagttg
    32461 ttttatgttt agaagaagta ttattatcca tattagaaga agatttaatt tcaaataaat
    32521 ctgtagagct tttaaattca tttatatatg ttaatagata tagcaatata aaagcatttt
    32581 gtgatgatac aaatataaat attaaaacat tggaaagaac ctgtttaaaa tatacagggt
    32641 atacacctaa gattttaaag agaatttaca gatttaaaat ggcaagtaat caacttattt
    32701 ataattataa agatgatgaa ctttttgatt ttatttatga aaatgagtac tatgaccaag
    32761 cacactttat aaaagaattt aaaaaatttt cagggacatc accaattaaa tttattggtg
    32821 agaataagac gattaaagaa aatataaagt acagctattt ataattatac tgtcgatttt
    32881 ttacaatacc tccaatttat tttttgatat gatataggaa aataaattgg aggttttaca
    32941 tatgaaaatt attacagaaa tagttgagtt taatgtggaa gaaaatttgt caaaagaaga
    33001 gtttatagaa attgtaaatg acttagaatt aaaatttcat tctattcaat ccggttttat
    33061 tgatactgag cttttatatg atgaaaagtc tcaaaaatgg attatgatac aacattggga
    33121 atctatagaa gacctcaaga gtgcttctag taaaatgttt aaagattctt caactgagaa
    33181 atttagaaaa gctttaaaga atcaaactgt taagatgaat ataataccac aaattaaaag
    33241 ttggaaatta tagaactgaa tgtaaaagat agttaaatag tattaaacta aattagccaa
    33301 ataacatttt gtatttaaat tgttatttgg cttttagtgt gagcgtggat tatcataatg
    33361 tcatattcgg gagctaaaag gcttaatact gatttgccaa ttggtcaatc catgcatgtc
    33421 catctggata tgctgttatt aaagccttta caagccaatc cctttgctct tgtgataata
    33481 gtgggactgt tacatcgaag tctttttgat aaccaactct atctatgtat attgatttgc
    33541 aaaacaaaac cacttcggga gccatatatg gaatattata tgatgttatc agtctggcct
    33601 tatccatatc tcttataatt ctttgatttt gggaaaaaag aaattttcca tctttctgtg
    33661 gattatatat caattcaata aaatcgaact gtaactgttc atcacactgt atattcattt
    33721 tatagttacc atcctcgact ttttttaatt caataaatga gcaatcgggt ttaattgcga
    33781 aagcacaaat ttgctctata tgttgcttgt taaggtcgag aataggtcgt aataaataat
    33841 tatccaatgg ctcatatata tcccatcctt gattgagcat atactgaatg ctcttttctt
    33901 tatctgtgtc agaaatccat atgtcaatat cgctgtgtgg gcgaatatgc atattcgcaa
    33961 ataattctag ggcatatcca ccacagattg aatatgaaaa gccacagtca ttaaaaaaat
    34021 ttacacaatc tttaattagt ttgttcataa tagcctcctt ttgcatctac tgaataaatc
    34081 aaatttttaa aaataccaac taaatgtttg ttttgtgtaa atataaaact gccgatatta
    34141 aggcagtttt atatttacta tttagaaaat ttattaacaa atttttgata ttcacacatt
    34201 atcttatcat tcataaaaga aatacccata aaagcagctt gctcaccaaa caaagtaaaa
    34261 cgttctatca tctgttgtgg attacctgga taaactgttg gaatacacca tgtgtttaga
    34321 agaaaaccaa acatttgagc agcaatctct ggatttttaa cttgcataga attatcttga
    34381 tttccttctt caatcaatgc ttttatccaa ggcacaatat catttataat ctcatcaaac
    34441 tgcgtagcta aatacttagg attacgcaat agtgatgatg acattaaaaa aatgtcttgt
    34501 ttattgtgtt gttcataagc atgaagcaac ctatattgta atttttctaa tgcggtcttt
    34561 ccttcatcat tttttacatc atatccataa aatccatcat tatacaaacg acttgtgatt
    34621 gccttaatta tttcatcttt tgatttaaag tgatgataaa tagcacctct tgaaaggcca
    34681 tctaaattat ctacaatatc ttgaatcgtt gtctgctcat accctttttc taagaacaac
    34741 ttatgtgaaa tatcaagtat tttttgtata gtattatcag gttctttttt tcttcccaaa
    34801 aagctcactc ctcaaaacaa tcatttagta tgtttatctt agcaaataca taatgatttg
    34861 tcaatgtgtc tttttagcat gttgaacagg tatattgcca tgctttcatt attttatttt
    34921 tttgccttat actggactat atttgcagtt tcctaatata tttggctcat atttaaatac
    34981 cattatctaa aagcactatt gctgtacgag aggcaatagg cattttagaa gaaggcggag
    35041 ttgagctaaa atatacatca taatctctta agttttcttt ttttgtgttt cctccaaact
    35101 ctaccatatc agtatttaat atcgttttgt agtttgtatt ttgaaacttt gaaatatcaa
    35161 ctgattgtat tagattaggg tggaaattaa atataaataa atatttgccc ttactgtaca
    35221 ccaaaatctt tttatcatta tccatatgta taagttcatg actattttgg ataaaaatat
    35281 cactgtattt tgtaagttca agcatagatt tatcaaaatt taataattgt ttatacttta
    35341 agttgttatc ttctgaaagg ctccattgtc ttctggcata cttatagctc caattgttgc
    35401 cttctcgtgg aaaatcaatc cactcaggat gtccaaattc atttcccata aaatttagat
    35461 atccttcacc tgctagagaa aaagttatta atttaattaa tttaataaga gatattgctc
    35521 tattgataac atggttattt gagtttattt ccatattcca gtacatttcc ttatcagcta
    35581 gccaaaaaat aattgtttta tcgccaacta atgcttggtc atgagattca caatatccta
    35641 tatttttttc tccaggtcgt ctagtagtaa gttcatacca cattttgccc aaatcccagt
    35701 cttcatcaga taagttagag atagttttta tccaaaaatc aggtactccc attgcaagtc
    35761 tatagtcaaa tccaattccg ccatctttta tagggataca cataccaggc attccgctca
    35821 tatcttctgc gatacttatc gaattaggat tgatttcttt tattaattca tttgcaaatt
    35881 ggagatatgt tatagcttca atatctgtat tcatactaaa atatttttca taactatcaa
    35941 atgaaactcc aagaccatgg ttacgatata acattgaagt tacaccatca aacctaaagc
    36001 catcaaaatg gtactcattt aaccaaaatt ttatatttga aagcaaaaaa tgtattactt
    36061 caggtttacc atagttaaaa agttttgtac cccaagcagg atgattcccc tttgaacctg
    36121 agtggaaaaa ttgatgttca cttccatcaa actcatttat tccttctaaa gtattcttta
    36181 cagcatgaga atgaactaaa tctaataaaa cagcaatccc cattgaatga gcagtattta
    36241 tgagattttt taagtcttca ggagtaccaa acctagatga gattgcataa aagtttgaaa
    36301 cttggtatcc aaaggatgca taatatggat gttccataat agccataagc tgaatggtat
    36361 tgtatccagc tttttttatc ttagaaagta ttttttcagt aaattcatta taagttccaa
    36421 tagattcgct ttcagtagac atacctatat gacattcata gattagtgga gaagttatgt
    36481 tttttaagtc aaaatcattg tcagtccaga taaatggagt ttttggctgc catatttgtc
    36541 cattaaatcc accaaaatct ttttgaatta ctctttttat atataatggt atacggtcaa
    36601 atgtttttcc atttgcagta acttgtacct ttacctctga tttgtgaggt aagctatttt
    36661 ggccaggaat aaatatttcc caattaccag aaccaatttt tcttagaaga tgagattttc
    36721 tattccaatt attaaagtca ccaattagtg acaaactatc tgcatttgga gcccattctc
    36781 tatagaacca tccatcattt gtctgatgaa atccatagta aagatgagca tttgcaaaag
    36841 aactaaaatc tttatagttt ttcaatgttt ttgatttcat atcctcataa tgtttcattc
    36901 gcttttttat atcagattca aatggttgta ggtatacatc aatatctaaa attttaaatc
    36961 ttttttgact tttcattgta tttctcctcc ataataaata ttacatctaa aaaataatat
    37021 ttatctcata ctatcttctc tgtataattg atatatatta tataatccta ataaaaagct
    37081 taagccacca atgattaatt tcataattta cctccctagt atttatactt atatatcgta
    37141 ttcatcaaaa tttaaaagtt tgtttaaaac tcttttaata atatcataat caaagccttt
    37201 ataggaaaga tgttgagaaa ttttttgata tattttcttt ttatcttcct tctttactct
    37261 ttcatacctt ttttttgcta gatacatagc attttcaaat tcagtgtcct tatctaattc
    37321 agatactgct tcatcgattg tacttctatt gataccttta ttatataaat tctgcttaat
    37381 tctgtttttg ccacatttat ttaggttaac atttgtgtta acaatttttt gggctaatac
    37441 actgtcattt acgagattat aacttttaag aaaatctatg accctctcaa ctatattttc
    37501 atcaaattct gatgatagtt tttcttttaa ttttttttca gattggtcag catgagataa
    37561 aatatttagt gctttatttt tagcttttat atacatttca tcatctaaaa taggttttaa
    37621 ttcttcttca tttatttcat tccctttttt taaattaaat gtataaacaa gttctttaaa
    37681 tatagcaata aaaaatttat catccacata aatattaact ctatcatcat ttcttttttg
    37741 ttgttctatt tt
//
LOCUS       DAEMAE010000011        36937 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEMAE010000011 DAEMAE010000000
VERSION     DAEMAE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.011"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1..1017)
                     /gene="ccpA"
                     /locus_tag="KPH93_000491"
     CDS             complement(1..1017)
                     /gene="ccpA"
                     /locus_tag="KPH93_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG1687328.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(1270..1470)
                     /locus_tag="KPH93_000492"
     CDS             complement(1270..1470)
                     /locus_tag="KPH93_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687329.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(1504..1683)
                     /locus_tag="KPH93_000493"
     CDS             complement(1504..1683)
                     /locus_tag="KPH93_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687330.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(1865..2071)
                     /locus_tag="KPH93_000494"
     CDS             complement(1865..2071)
                     /locus_tag="KPH93_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687331.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(2119..2544)
                     /locus_tag="KPH93_000495"
     CDS             complement(2119..2544)
                     /locus_tag="KPH93_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687332.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            2716..2943
                     /gene="acpP"
                     /locus_tag="KPH93_000496"
     CDS             2716..2943
                     /gene="acpP"
                     /locus_tag="KPH93_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG1687333.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(3087..4091)
                     /locus_tag="KPH93_000497"
     CDS             complement(3087..4091)
                     /locus_tag="KPH93_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG1687334.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFNIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(4105..5007)
                     /locus_tag="KPH93_000498"
     CDS             complement(4105..5007)
                     /locus_tag="KPH93_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG1687335.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSITHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGCVDEKLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(5203..6378)
                     /locus_tag="KPH93_000499"
     CDS             complement(5203..6378)
                     /locus_tag="KPH93_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG1687336.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTIVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     DKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(6466..7311)
                     /locus_tag="KPH93_000500"
     CDS             complement(6466..7311)
                     /locus_tag="KPH93_000500"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG1687337.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRADKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(7409..8206)
                     /locus_tag="KPH93_000501"
     CDS             complement(7409..8206)
                     /locus_tag="KPH93_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG1687338.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDVLMEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(8225..9235)
                     /locus_tag="KPH93_000502"
     CDS             complement(8225..9235)
                     /locus_tag="KPH93_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG1687339.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(9254..10036)
                     /locus_tag="KPH93_000503"
     CDS             complement(9254..10036)
                     /locus_tag="KPH93_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1687340.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(10095..11231)
                     /locus_tag="KPH93_000504"
     CDS             complement(10095..11231)
                     /locus_tag="KPH93_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG1687341.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(11940..12407)
                     /locus_tag="KPH93_000505"
     CDS             complement(11940..12407)
                     /locus_tag="KPH93_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG1687342.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSLKVYLDDSMKRFDSVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(12486..13028)
                     /locus_tag="KPH93_000506"
     CDS             complement(12486..13028)
                     /locus_tag="KPH93_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687343.1"
                     /translation="MNKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGINKCSAYVNIDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(13080..13766)
                     /locus_tag="KPH93_000507"
     CDS             complement(13080..13766)
                     /locus_tag="KPH93_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1687344.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANAKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(13766..14626)
                     /locus_tag="KPH93_000508"
     CDS             complement(13766..14626)
                     /locus_tag="KPH93_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687345.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENYPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIRESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            14761..15561
                     /locus_tag="KPH93_000509"
     CDS             14761..15561
                     /locus_tag="KPH93_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG1687346.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            15586..16155
                     /gene="yfcE"
                     /locus_tag="KPH93_000510"
     CDS             15586..16155
                     /gene="yfcE"
                     /locus_tag="KPH93_000510"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG1687347.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(16455..17507)
                     /locus_tag="KPH93_000511"
     CDS             complement(16455..17507)
                     /locus_tag="KPH93_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG1687348.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTKQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTETYIVGGEYSISKKLEGQLVNSERLAGTDRRDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(17714..18940)
                     /gene="pepT"
                     /locus_tag="KPH93_000512"
     CDS             complement(17714..18940)
                     /gene="pepT"
                     /locus_tag="KPH93_000512"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG1687349.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELAEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(19031..20083)
                     /gene="ytvI"
                     /locus_tag="KPH93_000513"
     CDS             complement(19031..20083)
                     /gene="ytvI"
                     /locus_tag="KPH93_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG1687350.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGGI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINEFMKSFQEIANFDVEQLVNKFSGEVMQMTKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(20232..21485)
                     /locus_tag="KPH93_000514"
     CDS             complement(20232..21485)
                     /locus_tag="KPH93_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG1687351.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(21616..25143)
                     /locus_tag="KPH93_000515"
     CDS             complement(21616..25143)
                     /locus_tag="KPH93_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1687352.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNLQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNNEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLVILNQELEVIKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIREVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKINKLEEI
                     LKEIENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLVLLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSILNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(25130..26341)
                     /locus_tag="KPH93_000516"
     CDS             complement(25130..26341)
                     /locus_tag="KPH93_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG1687353.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DDSEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(26386..30213)
                     /gene="addA"
                     /locus_tag="KPH93_000517"
     CDS             complement(26386..30213)
                     /gene="addA"
                     /locus_tag="KPH93_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG1687354.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRVSLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISNVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIIP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKASSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGVESLDTISQYEILKGKNFLDWIMPCVLKHRDLSN
                     LLEEVGLDAVFSVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSNY
                     YNEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(30213..33680)
                     /gene="addB"
                     /locus_tag="KPH93_000518"
     CDS             complement(30213..33680)
                     /gene="addB"
                     /locus_tag="KPH93_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG1687355.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EVENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLSRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGK
                     ESEFELELKEKINEIKNKVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDEDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKARKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLSWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYSPIKIVLENGQEVSLIGQIDRVDEFEEGENKYIRIVDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(33670..34500)
                     /locus_tag="KPH93_000519"
     CDS             complement(33670..34500)
                     /locus_tag="KPH93_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG1687356.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(34609..36828)
                     /locus_tag="KPH93_000520"
     CDS             complement(34609..36828)
                     /locus_tag="KPH93_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG1687357.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILVLTYMNSAVNNFKGRIRTILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQARYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKIINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNGLIEFNEESDI
                     CLGFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNKVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYTHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLNRLNQHIIEKRSN"
BASE COUNT        11376 a         6712 c         3698 g        15151 t
ORIGIN      
        1 ttattttttt ctctctgtaa cactttctct atcaacaatt ctataaggta attctatttt
       61 cttattttca attaattcct tatttattaa ttttataacc attcttattg ctactgctcc
      121 catatcgtat agaggttgat atactgtagt aagtttaggt ctatacattc tagctatctt
      181 aacatcatta aatcctgcaa ctgatatatc ttctggaacc ttatatccag catcacatat
      241 agcatttata gcacctatgg cagcctcatc acctgttaca aaagccgcat gaggtattat
      301 tccatcatct aatagttctt tcatactatt atatccactc tcataatcag ttccaccata
      361 cttaatcaaa ctcttgtcta actctatatt gttatttgaa agtgctttct cataaccagc
      421 aagtctttcc atttctaaaa cagtatcatc tttactagtc attataaaag ctatcttttc
      481 atgacctttc ttgataagat gctctgtcat atcaaaagta gcttcttcgt tgctagtact
      541 tactgtgtat atatcgtaat ttctagcagt tttacttata tatgtcgctg gtattccaca
      601 attttgtata tagttgatat gttcttgttc aactatccac gaaatcataa ctataccttc
      661 cacttgtttt gctctcaata gatttatact cttaagttct tgttccttat cagagtatgt
      721 attcgctaaa agaatatcat agtcatacat tttagcaacc tcttctatcc catttaacac
      781 ctcattaaca aaagaatctg aaacttctgg aactattacc cctattaatt gactcttctt
      841 tgttactaag cttctagcaa gtggatttgg tatatatcca gtctctttta taacctctaa
      901 aactttttgt ttgacttcat cagttacagg ttttgaatca tttataactc tagatacagt
      961 agatattgac actcctgctt gtttagcaac atcttttatc gttatattgc ctttcatctt
     1021 catcctcctt attttactaa atttatctaa aactgaacca aatttttgaa ggttcctatt
     1081 ttaactacat ttatataagt ttttatttta ccataaaagc aagtacattt aaatacatta
     1141 tattttatat tataccctat attcaacaaa tttaataata ttcattctta atttaaatca
     1201 tattaaaaaa tagaataaaa aaatgctggt ataaaatacc agcatttttt tattctaatg
     1261 atttaagtat tagcagccgc aacctacttc accatcaaag aatccaccac atgaacaagt
     1321 tattagcaat aatattaaga aaggtatcca tgacattatg tcaatgtctc tccagcaatc
     1381 ttcaaaagct aagaatagga agaatagtac tattatccac catcctcctc caccaaagca
     1441 tccttcttct ccaaaaaatc tatctaacat aacaaagccc tccttttttt aataactgtg
     1501 ttcttattct aagcatccac cacagcagca aagtattaat aaaactatta ggaatggtat
     1561 ccatgccatt atgtcaaatt ctgcccagct atctccaaaa gctaagaata ggaagaatag
     1621 tactattatc caccatcctc ctccaccaaa acatccttct tctccaaaga atctatctaa
     1681 cattatacgt tacctcctgt tgatattttg ttgagctttt cgctcctaac atatactatg
     1741 catttggttt ccattttgct aatgtatttt tactattttt ttaaattttt taatttgaac
     1801 taaaaaaggt aaaaccataa cttgtataag ttactggttt tacctattta ataatcaata
     1861 ttttttagca acaacaacca tcatcgaagt taaataataa caatactatt aatataaccc
     1921 atatccatat attgtcatcg cataataagt cttctaaaat gtctgtgcat aatagtaaga
     1981 atactataac tattattatc aacattttat tgccaaaaaa tccttcgcat cctccaaaga
     2041 aacgttccat attgtcttct aattttgtca taactaatcc tccttgaatc attaagagac
     2101 taagttttgt ctctttaatt aaatatatgt agtaatagac agataagtta cttctaaaaa
     2161 gtttttattg attagtttat ttatttcttt ttcaccaagt ttattgtatc tatcaacttc
     2221 atccagataa tcttttttct cctctgatat agtatagatt aaactttcta ttgattttat
     2281 tttgtactcc ttatatcttt ctatcaagaa ggatgttttt gaattttcta ttcctttctt
     2341 tacatgcatt aaccttgata tgtagtcttc tttttcatta gatacataac tctccttttc
     2401 agctacatct tcaaggatta gattggtctc cattacataa tcctctatac gttgcatatc
     2461 taaagttatt gaattataat aaaaatttac tgctataagt aaaaacattc ctactgaagt
     2521 tagtaatagt cctacatatt tcatagcatt tcctcctaag ttatatttgt tatcaataaa
     2581 gtttattcat caaataatac tttattacta catttataaa attttattat tttttataga
     2641 ttccatattg aaaaattctt gatattatat taaactaaca ttaaaattaa ttaaaactgc
     2701 tttaggaggc ttaaaatgtt tgagaaaata aaagaaataa tagcagaaca attaggggta
     2761 gacaatttag atgagataac tatggaagca tctttaatgg acgacttaga agctgattca
     2821 ttagatgctg ttgaaattat aatggccctt gaagatgaat ttggaataga aataccagac
     2881 gaggaagctg agaactttaa atgtattggt gatatatgta aatatatcga agaaaacaaa
     2941 tagtatcgtt ttgtctacaa atataaatag tctgtgtaaa cttctacata ataaattata
     3001 ttagagttcg cacagactag attacattta tttacaatat ataaaataca cgctcttata
     3061 tttaatttat atactaagta tataaattac ttttcttcat aaaacgtaac attctttatt
     3121 attatatcta tagttccatc ttgattttgt tttacagtat acttcattgg gtcttcaaag
     3181 tcagttagat tcccttttat gttaaaacca ttatccgttt ttatacttct ttttttaagc
     3241 tttttctcaa cccatttttt atctatacta aaactttcat caagaccttt ttcttccata
     3301 tgctctttaa agctatcttt taactcatca tctttaattg aattatcaac aaagtcattt
     3361 atatcaactt catgtttttc ctttagtgta taatttaata tacttcttac atcttcagct
     3421 tgctttatat cattactaag agcattggtt atccaatttt cagctgtatt tttaaatacc
     3481 ttggtcttgt acttatcatc atctattttt ttagcattta aaaattctgt tacaaactta
     3541 gattctgacc cttctttttc agcatcctta tctaaaagcc ttagatgaaa ctcatcattt
     3601 ataccactta aactaatcaa agctccttgc ttttgtctta aagtctctgg gataccaatt
     3661 tcatttgaaa ccatttgaat attaaattta tcatccacaa attcaattga atgagtgtac
     3721 aactttttat aatctaattt cagtatggct acactctttt catctttaat agtgtataag
     3781 caaattgcca aatcacaaga ttctagtgca gcattaattt tcataacatc aaacaagtaa
     3841 gatgcaattt ctttagaatt tttcaaaaaa gtactttcat catatattat ttgttcacaa
     3901 cagtttttaa ttaaattatc attataatct ttaaaaacac cttttcttaa atcctcatct
     3961 tttgcaattc tttttatagc tttctggaaa aaaccatcta cttcttgatt aactttgcct
     4021 tcaaagtcat ttaatattgg aacatcacta tttttatcca aaacatgtat tataaattta
     4081 tgtattatca tatatatcac cccattatct taatttcgat attaactctt tcatatcctc
     4141 tggtatatct gctttaaatt tcaatttttc ttttgttctt ggttgttcaa actctagctt
     4201 gtatgcatga agtgcttgcc tatttatgag cttttcatct acacaaccat acaactcatc
     4261 acctattatt ccatgaccta tatgagccat atgtactctt atttgatgtg ttctaccagt
     4321 ttctaagctt acttctaata cagttgcatt ttctagtctt tctattactt tatagtgagt
     4381 aatagatgac tgtcctcttt catctattat cctctttatt gaatcttctg taggtctata
     4441 aattggctca tctatagttc cctcattttc ttttataatt ccatcaacta ctgttatata
     4501 ctttttttca actttatttt cactcattgc tgtagacaaa gtatgatgtg cataagcatt
     4561 ttttgctact ataactaacc ctgaagtatt catatctaac ctatttacaa atcttatttt
     4621 tacgttttcc ttttgattat ctatatagta actaatacca tttgctattg ttttatcata
     4681 gtggcttttt gttgggtgaa ctaccataaa tggaggttta ttcaccatga ttatatcaaa
     4741 atcatcataa attatctgta aatttaaatc ttgaggttca aaatttgctt tttcctcgtc
     4801 tatttttact tctattaaat caccactata cacctttaaa ctcggttttt tatatactcc
     4861 atttactaaa acgctctttt ccctcttcat ttttgataat gacctaactg agaaatttaa
     4921 cttgtccaat aaaacttctt ttaaagtcat ttcttcttca tttgtatacg atataagatt
     4981 atatctttga ttctcttttt taaacaaact tttacctact ttcttttata tcttcctact
     5041 tttttatatc ttcaaaatca tgatattata tattttacac tatttttgtt taatagataa
     5101 taattatttt taactataaa aacctgtaag aataacaaat agttattctt acaggttaaa
     5161 ttcatagtta aatatttaat ttgtaaatat tataatctaa aactatctct taactattaa
     5221 agtagttccc attccaccac ctatacaaag tgtagcaaga ccagttttag catctcttct
     5281 cttcatttca tataaaagtg tagtaagtat tcttgctcct gagcatccta ttggatgtcc
     5341 tatagctatt gctccaccat tgacattaac tttatccata tctatattta agtctcttat
     5401 tacagctaca gattgagcag caaatgcctc attagcttca actaaatcta tatcttcaat
     5461 agtcatatta gcagcttcta aagctttttt agttgctgga actggtccat atcccattat
     5521 tttagggtca acaccagctg ttccataaga aactatagtt gcaagaggct ctattcctag
     5581 ttcttcagct ttttctttag ccattactac tagcatagca gcaccatcat ttattcctga
     5641 tgcattacca gcagtaactg ttccatcttt tttaaatgca ggtcttaact tagcaagttt
     5701 ctccattgta gtgccaggct taatatattc atctttatct actatagtgt caccttttct
     5761 tccttttata acaacaggaa ctatttcttc atcaaatttt ccttcagctt gagctttttc
     5821 agctttattt tgacttgcaa gagctaattc atcttgttct tctctagtta tattccattg
     5881 ctctgctatg ttttcagcag taatacccat gtgatagtta ttaaatatgt ctgataatcc
     5941 atcttttatc attgaatcaa caaaagcagc atcacccatt cttgcaccat atctcgcact
     6001 tggtactaaa taaggagaca tactcatgtt ttcagctcca ccaactaaca ttatatcagc
     6061 atcacctaat gctataagtt gagatgccat tgaaacagat cttaatccag aaccacaaac
     6121 tatatttata gtcatagctg gtttttctac tggtatccct gctcctaatg ctatttgtct
     6181 tgctatattt tgtccaagac ctgctgtaag tactccccct aaaagagatt catctatcat
     6241 atctggagtt atattagctc tttttatagc ttctttagct gctgttaccc ctaactctac
     6301 cgctgaaact gatttaaatg ctcctccaaa acttcctact gctgttctag ctgcactggc
     6361 aattactact tctctcatat tcattacccc cattaaatta gattttccat atatccgaaa
     6421 ttgttttttt aaacaatttt atttaaattt ttaattccca aactcttatt tattataatc
     6481 atagaatcct atcttagttt ttcttcctaa ttgattagct ctaaccattt tagctaaaag
     6541 tggatgaggt ctatatttag tatctccaaa ctcagtatat aaaacattca ttatagctaa
     6601 aacaacatct aatccgatta aatcacctaa tgctagtggt cccattggat ggtttgctcc
     6661 taatttcata gcttcatcta tttcttcttt acttgcaaca ccgtctgcat atataccaac
     6721 agcttcattt atcataggta taagtattct atttactaca aatccaggag attcagatac
     6781 atctactggt actttattga tactcttaga taattcaaat actgtatcaa aagtaacttt
     6841 tgatgttagc tgaccactta taacttcaac taatttcatc ataggaactg gattgaaaaa
     6901 atgcattcct ataactttat ctgctctctt agtagaagaa gctatttctg ttatagataa
     6961 tgatgaagta tttgttgcca agatagtatc ttctttacat aattcatcta gtaacttgaa
     7021 aacatctttc tttatattca tgtcttctac agatgcttct attattaaat ccatatcttt
     7081 taaatcttca tagttagtag ttgaactaac atgacttaat atttctgctt ttgtagcttc
     7141 atccattttt cccttagtaa ctaacttagt taaattttta tctaataaag ctaaacattt
     7201 atctatagca ccttgagttc tactctttaa acatacatca tgtccacaac ttgcaaaagt
     7261 ttgtacaata ccacttccca tagttccact acctattaca gctaatttca tagttataac
     7321 ctcctgttat tttagtattt atattctgtt aactagatta aaatactatt taatctagtt
     7381 aacagcaaat atatttttaa ttagattttt acccttttat aaagttagct tctctttttt
     7441 caacgaaagc tgacattcct tctttttggt ctttagttga aaaacaaaga ccaaataaat
     7501 tagattctat atctattcca gtatttatat cagtttgagc accaagttgt attgcttctt
     7561 tagagtatct aactgcaagc tgagcatttt tggctattat cttagctagt ttctcaactt
     7621 cttccattaa aacgtctggc tcaacgactc tatttactag ccctattttt tcagcttcat
     7681 cagcttttat aacttgacct gtaaagatta attcttttgc ttttgccatt ccaaccaatc
     7741 ttgtaagtct ttgagttcct ccatatcctg gagttattcc aagagttact tctggttgac
     7801 caaatttagc tttagcagat gcaattctta tatcacatgc cattgcaagt tcacatcctc
     7861 cacctaaagc aaatccattt acagcagcta tcactacttt tttactattt tctatttctc
     7921 caaaagcttt tgctcctaag atactaaaat ctttagcagc tacagcatct aaatctttca
     7981 tgtatgcaat atctgctcca gctacaaatg cctttccttc ccctgtcaat attacaacat
     8041 caatagtttc atcattatta atatctacaa atacttcata aagttcttct aaagtctttg
     8101 aatttattgc attaagggct ttaggtctat tcattttcac tgtacatata tttccatcta
     8161 cttcaacagc tacattctca taaactttaa catcacttgt actcataata ccctccccaa
     8221 gaaattagtt agctaaaact tcaccttttt cttttgcaac acttaactga ctgataagtt
     8281 ctggaagcac tttatgaaca tctccaacta taccaacatc agcgtattta aatattggag
     8341 cttctggatt tttatttata gcaactataa actcagcatc ttccatacca gctatatgtt
     8401 gtattgctcc agatatacca catgctatat aaaggtctgg tcttacagtt ttaccagttt
     8461 gaccaacttg tcttgcttta tctaaccaac ctgcatctat agtcgcacga gaaccagaaa
     8521 cttctccacc tataatttca gctaattcat aaagtatgtc taagttttct tttccaccca
     8581 ttccacgtcc agcagaaact aatatcttag catcttctat tttaacttgt tttttagctt
     8641 cttttattac ttgtacaact tgaactaatt tatcagcatc attaaattct accttgaaac
     8701 gattaattac agcttcttta gtttcatcag gttcattttt cttcataacc cctggtctaa
     8761 ctgtagacat ttgaggtctg aaatctttac aaactattgt tgccattata tttccaccaa
     8821 aggcaggtct tgtcattaat aataattttg tatcttcagc tactgcaaga cctgtacagt
     8881 cagcagtaag acctgtatgt attctagcag aaactctagg cgctaaatct ctacctattg
     8941 aagttgcacc aaataataca actatagggt cagctgcttt tattgcttca taagctgctt
     9001 ttgtatatgg ttcagttgta tacactgcta aagcttcatc atctactact attacctcat
     9061 ctgcaccata gtgtgctaat gtatctatta aaccttctac cttactacct aaaagcaatg
     9121 cagaaacttt tgtatcataa tcttttgcta tttctgtagc ctttcctagt aattctaaag
     9181 aaacagtttg aattacattt tctctttgtt ctattactac taaaacgtta cccattcttt
     9241 atcccccctt aaattatatg atatacttct cttttaattt atctatgata attccagctg
     9301 atgttttcgc atcttcattg tatattgtac cagctggttt aactgatttt gtaaatgatt
     9361 taaatacact agttggagaa cctttaagac ctaaattaga agggtcaact tctatatctt
     9421 ttacagtcca tgtttctact acatcatttt cgaaagcatc atatattctt ccaactttca
     9481 tgtatcttgg tgtgttcata tctttaagag ttgttataag gcatggcatt ttaactttta
     9541 agtcatggca acaatcttcg aattgtcttt ttactaatac atattcacct tcagttttta
     9601 tttcttcagc atatgttatt gatggaagat ttaaatgttc agctatttgt ggtccaactt
     9661 gtgcagtatc tccatctata gcctgtcttc cagctattat aatatcaaaa tctatatttt
     9721 ttaatgctcc tgctaatgct gatgaagttg cccaagtatc agcacctgca aatgctctat
     9781 ctgttaataa tattcctcta tctgcaccca ttgctaaagc ttcttttaaa gccatatctg
     9841 cttgaggagg tcccattgtt ataacagtta catgagcacc catttcttct tttaatttta
     9901 tagcttcttc taaacctgct ttatcatcag ggtttattat acttggtact ccatctctaa
     9961 ttaaagtacc tgtattagga tctagtttaa cttctgttgt atctggaact tgttttatac
    10021 aaacgactat attcataaat ccttcctcca tatctatttt aatatcgacc tattgtgtat
    10081 attttaatat ttatctattt taataatttt cctgaaataa ccattctttg aacttcacta
    10141 gttccttcat atatttcagt tatcttagca tctctcatca ttctttctac tggatagtca
    10201 cgagtgtatc catatcctcc atgaagttgt acagcttttg tagtaacttc catagctgtt
    10261 tcagctgcaa ataattttgc cattgctgct tctactccat aaggttttcc taagtcttta
    10321 tttatagctg cttgatatac aagatgtctc gccgcttgta ctttaacttc catgtcagct
    10381 aattggaatt gtgtattttg gaattttgat aatggtctac caaattgtac tctttctttt
    10441 acatatttaa cagtttcatc aagagcacct tgtgctaaac ctaaagcttg tgcagctata
    10501 ccaattctac caccatcaag agtagacatt gctatcttaa atccttgacc ttcttttcca
    10561 agtaaatttt ctttaggtat tctgcaatcc tcaaatatta actcactagt agctgaacct
    10621 cttataccca ttttcttttc tttaactcca aagctaaacc caggagttcc tttttcaact
    10681 ataaatgctg atattccttt gttcccctta gatttatcag tcattgccat tactacatat
    10741 atgtcaccag ctattgcatt tgttataaat atttttgagc cattaagtat gtattcatcc
    10801 ccgtctaaaa cagcagttgt ttgttggcca gacgcatctg taccagcatt aggctcagta
    10861 agaccaaatg ctcctaattt ttctccactt gctagtggtc ttaagaattt ttgtttttgt
    10921 tcttcattac catattgata tataggccat gagccaagag atgtatgagc tgataatata
    10981 actcctgtag taccacaaac tctagacaat tcttcaactg ccattatata tcctacagtg
    11041 tctccacctt ctccaccata ttcttttgga tacggtatac ccatcattcc tgcttttgcc
    11101 atcttttcca ctgtttcata aggaaatctt tcttcttcat caagttctgt tgctaaaggt
    11161 ttaacttcat tttcagcgaa gcttacatat aactctttaa gcatctgata ttttttagaa
    11221 tttaaatcca taatagcctc ctcagtattt tataaatatt ttattttcat ttattaatta
    11281 aaatctcggc aacatctata ataaggtatt tgaatatacg tcgagaaatt tataacctta
    11341 attaaactac gctctatatt tatatgtgat tatactaacc aattatgaca ttaatttaat
    11401 cataaattgt taatttaaaa tcaactatac ttaaaaaaaa tctcgctttt tggaattttg
    11461 tgaaatttta agttagaata agatttcttt cacactggtt acagataaaa taatttgttt
    11521 acgatattta tattagggta tattaaacat taaatattgt gtgtatatct gttttttagc
    11581 catgtacaga agatttttat cgtataaaaa ttattttaat agagctaaat aatcgtattt
    11641 ttatctgact attttttatt tttaaatatc ttacatctat aatagtatca ctcaaaggat
    11701 aatttttcaa taatttctaa ttattttttt aacaatattt attacatttt ttccactttt
    11761 ttgtcatttt tttattgttt tgaaaaaaaa tcagaatttt ttaactttat tttggattat
    11821 tagtttaaat tagattttct tcttagattt ataaatattt acacaaaaaa actacctctg
    11881 tatagattgt atacagagat agttttttac taattcctac tatagtttac tatatttttt
    11941 taccaattct tacaaacatc tacccatttt tcacacttag aaaacttctc catttcttca
    12001 catgtaagtt ctattgcaga gttactactg ccacatgcag gaaatactga atcaaatctc
    12061 ttcattgaat catctaaata cacttttaga gaatttttta aaccaaatgg acagactcca
    12121 ccaatagcat gtccagtaaa ctccaagact tcttctggcg tcaacatttt agccttacaa
    12181 ttaaattcag ccttatactt cttattatca atcttagcat caccagctgt cacaattaaa
    12241 attgcatcat ctcctatttt gaatgagagt gtcttagcta ttcttgctgg aataacccca
    12301 gctgcttcag ctgcaagttc cacagtagca ctagattgtt caaattctaa tatactgtct
    12361 tccttgccaa attgtttaaa atactctctt acattgctta ttgacattaa atatccctcc
    12421 tagtctgaac ttttataaat ttcatattat tcttaattat atctataatt gatttatttt
    12481 tcaaattatt ccataatatt agcattccat gtatcattat tttttccaac tactagataa
    12541 agtccttttg ccattggaat attgacaata tattctccct tatattctac actcataccg
    12601 gattcattaa agtacctata atcaaaattt agtccttttg gaattacaaa atcaacataa
    12661 ccttgattat ttggttttat tccatatttc aaagtccttt cagttataga atttttttca
    12721 ttaaataaat ctatatttac atatgcagaa cacttattta ttcccttttt attttccaca
    12781 caattttcaa acttcatttt agcactgttt aatttataac tatatttact attattgctt
    12841 attttaaatc ttacaatagt accaatatct ctacttctcc tgctatcaaa atcaaaaaca
    12901 atttcctcta aatgatttat ttcataatct ttgacactta ccatatctct atatactaat
    12961 ttatttatag ctatgaatag tgtaaaaatc cccaaaataa aaaatgaaac ttttataaac
    13021 ttattcattt ttagtctcct tatattttgt gctattgtgc tataatatta attaaaattt
    13081 taataattaa acttattctt ttttgaaatc ttaatagaaa aatacattga tattataaaa
    13141 attattattg ttaaaaatgt catactatat aaatttatat acttagcatt agctatatca
    13201 gttacataat ttgaaagata tactcctcca actttactat aattaaaatg ctttacttga
    13261 tatacaatca tactaatcat cttcaaaaga cttccatata caaagtaaat taaaatactt
    13321 actataaaac cacaaacttt catttttcca tatccaaact taaaaattaa aggaagtaaa
    13381 atcgtcaaaa ttgctaaaaa tatatttaca gaaaaaaata catcatttaa aaccatattt
    13441 ctaaagaata ctagtgatgt caatttactt agtaaggaca caattattgt tacagaaatt
    13501 attagtccta cacctaataa atattttgag taaactatat cttctatact aactggcata
    13561 ctccttataa aaatccttga atcattttca taatcacact caaacgtatt tataaatatc
    13621 aaataactaa caacaatact tactgtataa tatgaaattg aattaaggaa aaaataaaac
    13681 acaaaaaata gcaatatgta ctttaaatta cgaataccat ctgaactaat ccctattatt
    13741 aaatcttttc ttattaaatt gaacatcatc tcacctcatt tacataataa tacattagtt
    13801 cttctatagt aggattttta accttatgct tatcaatttc tatcaataag ctttttactt
    13861 tcaagctatc tccaacttta acaagtgcct cattataata tttatactct ttcacaccaa
    13921 ctatgctttc tctaattgct tctaactctg ctttatttgc cctaattatc ttatattctt
    13981 gttctattaa ttctttctta accgtaaaaa tctgatttcc tctatttata aaaacaagat
    14041 aatcagcaat tttaattaaa ttatcagtat tatgagtaga aattattaca ctactacttc
    14101 catttttcac aaaatcttgt aaatactgca ttatttcagt tcttacaatt gggtctatcc
    14161 cagcagttgg ttcatccaaa atcaataact tagcattgtg actaagtgcg cttgataaca
    14221 taagcttttc agattgacct ttagataaat tttcaatatg catattttct tctatatcaa
    14281 acttattaag gtattcatca aacctttctc tatcaaaatt cttataaaat aaagaaacta
    14341 tttttctaaa ttccttaacc tttaaatgcg gataaaattg taaagaatca tatacaaaac
    14401 caatattgtt tttaaagctt attggataat tttcaatagt ttcattacaa agttcaattt
    14461 caccttcatc tgcatcaata atccccatta taatttttat taaagtcgtt tttcccgaac
    14521 catttggtcc tataatccca acaatatccc cttcctttaa ctctaagttt atattattta
    14581 acttgaattt tcctaatttt tttgtgatat tccttatgct aaacacatct tcacctctta
    14641 tgcatttttt tatacccacc tatacatatt ttaacacatt cactacaaag gtcatttata
    14701 aatttataaa ttaagaatta taaaactatg ttataataca aagtatgaag aggtgaatat
    14761 atgaaaagaa ttataactat aaatacaaac caattaaata agtcccttga gactaaagaa
    14821 ctcttaacta gaaaacttat caatgctggc tttgaagtgt attcagatat ttatccagat
    14881 acagagctta ttatatcaat tggtggtgat ggctcttttt taagaacagt tagagatttc
    14941 gattttcctg agattcccat aatgggaata aatacagggc atcttgggtt ttttcctgat
    15001 attttaccag ataagattga tagttttatt gaagcttata caaaaaaaga ttatattatt
    15061 caagaaatgt cacttcttaa tgcggaagta tatacaacta ctagtggttc taatatgctt
    15121 gctgtaaatg aggttgttat aagaggagac aagtctagga ctatacacct aaatttaagc
    15181 cttgataata aacatataca aaattttagt ggagatggta tgattatttc tacttccaca
    15241 ggttcaacag cctataacta ttctgctgga ggtagtattg ttgatataaa tcttgaactt
    15301 atgcagatta cacctttaca tccgataaat accaatgcat ataggtgctt tacatcaagt
    15361 ataatatgtt caaatgaatc tgttataaag attgcaccag aatatagatt tgaagattct
    15421 gtattgattg tcgttgatgg agttgagcac agatttagac aaatcgaaaa tataaaagtt
    15481 agtatatctg atgcaaaaat aaaattactt agaatgtcaa actatgagtt ttggcataga
    15541 gtatctgaaa aatttttata gactgtactt aggaggattt ttaatatgaa aataggaata
    15601 atgagtgata ctcatggtag cttactttac tttgaaaaag cattaaatgt attatctgat
    15661 tgtgatgttt tattacatgg tggagatgtt ttatatcatg gacctagaaa tgatattcca
    15721 gaaggatata atcctaaaaa atttattgag acattaaata aattagaaaa cattgtgatt
    15781 gtaaaaggga actgtgatgc agatgttgac caaatggtta tagaacatcc tattcagagt
    15841 ccttatgtca tgagccaatt tggtgaaatt agaattatat tgaatcatgg ttatatagaa
    15901 tcagaagaag aaattattga caaagcaaag aaaatgggtg gtgacatatt agttttagga
    15961 catactcatg taaaaaagtt atacatggat gataatttaa tagtaataaa tccaggaagt
    16021 acttctatcc caaaagatgg aagccattct gtcgcaatta tagatatcat aaaaactgat
    16081 gatgaggatg agcttgaact tgatataaac ttaattgaca tcaatactgg aaatataata
    16141 aatattaatg attagtaaat ttttaataaa aataaaataa gctttaattt attaaactca
    16201 taataaaaaa caagtaaacc attttatttc tatctcaatt agaagtttaa gtaaaacacc
    16261 ttaaacttct aattaaatag actctggttt acttgtttta ttatatgcta tattgaaata
    16321 taacttagtt tacttgtttc tattatatat tatattgaaa catagcttag tttacttgtt
    16381 tttattatat attatattga agtgcaactt ggtttacttg ctattattat atattaaatt
    16441 tgatttacac aatattactg taaggctaat gcttctgcat atggtttact atttttgcct
    16501 ccaccaactt gtacaaattt atcagccttt acttttttta taaaactttt ctgtgcacta
    16561 cttaaacttc cacttgcaag tactacaggt gactttgttt ttgatgctaa tactccagca
    16621 gcaagcccat caactaaatc agcttctctg ccacttccat cttttgcaaa atacatatta
    16681 ttaaccttac ttgattttcc ataaaacttt tctaaaactt ttgcattagt atctcttcta
    16741 tctgttcctg ctagtctttc tgagtttact aactgtcctt caagtttttt acttatactg
    16801 tactccccac ctactatgta tgtctcagtt gagttttgtg taataaaatt ctttgcctca
    16861 cttcttatgc tatctttacc tgtatatatt atagctgtat tatcacttgc tgctgggctt
    16921 gatattgaag ttgcatctgc cagtccatta tatccattaa ccacagctac ttttgagaca
    16981 cctaagtcac cagtgtcatc taactgctta gtaagaagta aagatgtttc ctctctatct
    17041 gctccaccta ttctactgac taagacttcc atatcttcaa gagattcttt tacacttgat
    17101 gaaatagctc cttcacttcc aattaaaata acttctgctg gattaagcct ttttatttcc
    17161 tctgttacat tatatggtat actattttta tttgtaagca gtattggtgc atccaattta
    17221 cttgccaaag gagttgaagc caatgcatct ggcaatgaat ttgagttaac tagaaacacc
    17281 ctttctgaac cattattcca accactttga gatattttta tcgctgtatc aaatctatct
    17341 tcaccaataa gtttttcctc ctttggagat tttctacctt gaagataatt attcatatct
    17401 acctgtgcat tttgattttt actaatagtg tctgcatata cacaacttgg caacataatc
    17461 ccaagtgaaa gtgcaaatgc tattattttt ttagatttaa ttttcaatac aattccccct
    17521 tttaaatgtt aatacctatt gatattcata ctttttgttg ataccctaaa ctaaaaataa
    17581 ttatctttta taaaaataat cataaaaata gctataccat tttaaataca atacaagctt
    17641 ataatcagct tgacttataa agaatgatat agctattata actttaaata cttaattttt
    17701 aaatactttt taactaaact ctttcagcat atttttcagc tattcttaca attagtttag
    17761 tagctttctc catagaacta actggtatac actcattttt cccgtggaag tttaatccac
    17821 cagtaaatat atttggacaa ggtaatccat taaaagataa tcttgctcca tcagtaccac
    17881 ctcttacagg aactaaacgt ggttttatac ctaattcctc catagcttct ttagctatat
    17941 caactataaa cataactggc tcaatctttt ctttcatatt gtaataagag tcttttaaat
    18001 ctaattccac tctattatcg tatttcttat ttattttttc tatattctct atcataatag
    18061 attttctatt ttcaaatttt tctctacaat ggtctctaat aatataagcc atacttacat
    18121 tctcgacatt tccacctata gaatttaaat gccaaaatcc ttcatatcct tctgttgttt
    18181 ctggtctttc atctgctggg aacatttctg atatttcagc agctatatgt atagcattta
    18241 ccattttatt tttagctgaa cctggatgaa catttctacc atgtatagta atagttgcat
    18301 ttgccgcatt aaaattttca cattcaagtt ctccaactat tcctccatct aaagtatatg
    18361 catattttgc tccaaatttt tcaacatcaa ataaatcagc acctctacca atttcttcat
    18421 ctggtgtaaa tcctattttt atatctccat gcttaatttc tggatgattt attagatact
    18481 ctattgcagt cactatctca gctataccag ctttatcatc agacccaagt agagttgttc
    18541 catcagtgac tattaaatcg tcacctttaa attctgccaa ctctggagaa tcctttacat
    18601 acgtaataac atttaacgct tcatttaaaa ctatgtcttt tccatcataa ttttctatta
    18661 ttcttggttt aatatctttt ccactaacat cttcaattgt atctaaatgt gatatgaatc
    18721 ctatagtatc cacaccatca gtatttcctt ttaaagtagc cattatataa ctattttcat
    18781 ctaaactcac atcatcaaca cccattgttt ttagctcttc aactatatac ttagctaaaa
    18841 ctctttgacc ttctgaagat ggacaattct cacactgaga attagatgtt gtatcaaatg
    18901 atacatattt taaaaatctt tccactactt tttgtttcac gtatataccc tctttcttaa
    18961 taagattaat aattttttta tttaccagtg aataaagttt tatttttaaa aaaactatca
    19021 attattataa ttaaaaaata tccaccttct taagtatatc atgcatacat accaaaccca
    19081 aacaaaaaat cactccaatt attcctttta cttggattcc aataaaaatc gctgctaggg
    19141 tagctaatgg atttagtcca acatttaccg atactagttt aggttcaagt atttctctaa
    19201 tcaaagacaa aacaaaaaat atcacagtca tacttatagc tataaaataa ttttttataa
    19261 gaaaataata aacaattact ggaagatata ttgttattat acctagaaat ggaattaaat
    19321 ccattaaagc tcctacaaac cctactatta gaccgtattt caatccaaat aaagcaaaac
    19381 tcccccagat tgcaaaaaat gttatagcca taagtattgt ataggccctt atatatccaa
    19441 caagtgaaag acctgcctct ttctttacgt tttttacctt ctttctaaca tcaacagtaa
    19501 acatattata aaaactattt tcaatcttat caaggtcttt tgctataaag tatgtagcaa
    19561 taaacaaggt aattataaat attataatat atggaataga agtagccaat ccaagtatac
    19621 tagtaagaag attttttgtc atttgcatta cttcaccact gaatttgttt actaattgtt
    19681 ctacatcaaa gttggctatt tcttgaaatg atttcataaa ctcatttatt ttatttaaaa
    19741 aatacataat agtattactt atatcctcag gattagcaga tatattattt aaaaatccca
    19801 ttaattcttt cattgaattt attactatta ttgtagtaac tgtactgact atagccactg
    19861 caagaaaact tagaacaagc gtagagatac ctttatttat acgaaactta ttttctaact
    19921 tttgcgatat aggattaatc ataagtgcta ttataccacc tagaaaaaat ggagctatgt
    19981 atggaaaagc tttgtaaata aaaataaata acaatgtata tatacaaaaa aagatagtgt
    20041 tgttttttaa tttgtataaa aaattatttt ctagtatgct catcttaaaa atctcctttc
    20101 ttctaattta tatttttatt ttgtccattt catactagtt ttatttgaat ataaaataaa
    20161 aagaagtgca gaaatataaa ggttatcttt tatatttata cacttctaat aaatttaaga
    20221 acattattac attatgtata tattcttatt tttttaattc tatttttttc tatactttct
    20281 atacaaaatc ttatattatc aacttcaatt acttcatttt cttctggcaa tctgcttagg
    20341 tgtccaatta taaaacctcc tatggaatca aactcctcag attccaagtt tacaccaata
    20401 agttcattaa catcaccaat tttagtactt ccatctacaa tatactcatc ttcttttata
    20461 acttgtattt catcctcttc ttcatcatat tcatcctcta tatctccaac aatgacttct
    20521 actaaatctt ctatagttaa aagtccagat gttccaccat actcatctac aactatagcc
    20581 atctgacttt tttcaagttt catctcttca agaagttgtg ttattttttt gaactcatac
    20641 gtgaaaaaag gctctctcat ataattttta atatcaaatg actctatttc ttcatcagat
    20701 aaaaatataa tatcttttat attaagtatt cctacaatat catctatagt ttcttcatac
    20761 acaggcattc tacttaattt ttcagtttta aatacctgta ttatctcatc ataactatct
    20821 tccatatcta tagctaccat atctattctt tgtaccattg catttttagc ttgcatatct
    20881 ccaaattcaa aaacattatt tatgatttct ctctcttcca tttcaaggac gccttcttca
    20941 tgacttacat ttaccatagt ttttagctct tcttcagtta tgaatgattt agctccttta
    21001 tttgttatac caaataattt aaatataata tttgtaatta tattaaatat ccatactatt
    21061 ggtcttaaaa taaatataat tgcttttata ggtttagata ctaatagaga aaccttttct
    21121 gaattatttg ctgctattgt ttttggagta atctcaccaa atattaatac taatactgtc
    21181 ataacagctg tagcaagtgc cactcctttt tctcccatta atcctataaa caaagatgtt
    21241 gatatggatg ttgctgctat atttacgacg ttatttccaa ccaatataga acttaaaagc
    21301 ttatttggat tttctattaa tgaacttact aacttagctc ctttaactcc ttcatcttgc
    21361 atatatctga ttctgatttt actcaacgac attaaagccg tttcagatgc tgagaaaaat
    21421 gcagacccta taagtaatac tattaaaaaa ataatctgaa tcaaattatt gggcgattcc
    21481 aacacttacc acactcctac ttacatgttt ttatattaat attaattata aataacttta
    21541 atattagttt ttattatatc acaattacat attatttgga aatattagat aatatattca
    21601 attaacatat attttctaac tatattcaat ttttactttt gaacctattc cagcctcact
    21661 agatgaaact aatagcttaa caggaactct atttttaagt tcctcgacat gacttattat
    21721 acccacactt agattattac tatgcaatct ttccaaggat tctataacaa tttctaaaag
    21781 ttcactatct aatgaaccaa aaccttcatc taaaaagaaa aattctaatg gtgcacttcc
    21841 ctttaattgt atttgagatg aaagagcaag tgctaatgat agagatgtca agaatgtctc
    21901 tccaccagat agagtgtcaa tgcttcttct ttctcctcca ttaaagttat ctctcattac
    21961 aaagtttagt gttgagtcaa tttcaagtgc atatcttcct tttgttattc catccaatcg
    22021 ttttgatgct tccaacgcta tatattttaa ttggtttgta gaaacatatt ctacaaattt
    22081 atttccttgt acacatttat ctaattcttc caataagtcc acattgtgtt caactacctt
    22141 aagttgttta ttaaaatcat ttatcttatc taaagagtct tttaatgtta ttaactgatt
    22201 ttgattagct ccaatatctt tggatatatt tcctatttct actttcaaat tataaatttc
    22261 atcttttaac tgttcaaagt actccttctt tatgcttctt ccatttaatt tttcttttga
    22321 tgattttatt ttaaatgata ataacttttg ttcttcttca tgctctagta tttcttcaat
    22381 caattttttt actgcatcag tatctaataa agctcttttt actgcatata tactttcaaa
    22441 tttattttct gccaatagtt tatttagtat tgatttttgg tctttatatt gttccttagc
    22501 agttttaagt ctaccatcta tattgtgctt ttcagcaatg tttttttcat attctaatct
    22561 ttgctcttcc aattttttct tacttgattc ttcttgttct aatatcttac atatattttc
    22621 ttcaacatta tgtaaaagat ttttagctaa atcacctttc gttatgttaa taacttcttt
    22681 atacttatta tctctactca atttcttttc tacatataat tctctagcct ttattaattc
    22741 tatctctatt tcatgtagtt tgctctcatt ttctcttatt ttaatatcta gtgagtctct
    22801 attttttaat aaatttgaat gcccagtact tagtaattct aattcttttt cattctctct
    22861 tatttctttt acttttgaca atagattaga gactttagta atcgtcttta atcctagata
    22921 ctcctgttta agttttttac atttattttc taaaccttct atttctatgt ttaaatctct
    22981 tgttaatttc ttataattat tcagactctc tcttattttt aattcttctt tttcaatttt
    23041 atttttttct tctttagcta atgttacttt attttcagta ctctcttttt ctttctccca
    23101 ctcctgaata ttagatttta acaatacaag ttttctttgt tcttcatcaa gtttttttaa
    23161 taattgacta gatggaatcc cccctagttt tccttttaca tcttcaagtt ctttagtctt
    23221 cattttttct atagataaat acccacttac tttagcattt aattcctcta tattgtttct
    23281 tatgcttatt ttttcttttt ctaatttctc cagtttttct ttgacaaaag atatattttc
    23341 atcatactta gttatattat gattttcatg atgttttgac ccacaaactg gacaaggcat
    23401 attttctttt aattcacgtc taagttcaga ggctaaattt aaatacttta gttcttctaa
    23461 ttctttttct aggtcatctc tgttctttct attattttcc agtttttcat ttagtaaatt
    23521 tacttccctt tcagtattaa acttactttc aagtattatc tttaattcat cttgtatact
    23581 acttttctta atttcatttt ctttagtatt atttgcttta tttcttaatt cagtaacata
    23641 ttcagatttt aaaagtaaat ctgcactctt accaggacat tttttaagaa gaacatccaa
    23701 gtgaagtgat aaattttcta aattacgatt tacatcattc ttatctttat caatatatct
    23761 aacttttaaa tttatatttt ctatctcttt gagtatttct tctagtttat ttatcttttg
    23821 atttttttct tccaatacgt tgttgtaatc tttctcgtat tcatatgcta gaaatatttt
    23881 ttgttttaat tctgcactta tatttacttt atcaatcttt ccttcaactt ctcttataga
    23941 tttagtaact gcatcttttc ttgactctga cacctgcttt actttttcta attcaacttt
    24001 agtcttattt aaattaattc cactttcttt tagctctttt aactctctat ctaataaaac
    24061 taactcttct tctaatttta ttgcttgttg caatcttatt ttttcttcac tcaatttagg
    24121 gacttcttca ttcttaattt tgtttatttt ttcatatcta ttttttataa cttcaagttc
    24181 ctgatttaat attactaatt tcttttctaa attacttact gcaaagctat cttcatctat
    24241 ctttttttct agattttgaa ctgaacttat gtatgggtct acctttttag cattactatc
    24301 attttctaca agagcaatct tttcttttat ctcattattt tttaaatcaa gctcattttt
    24361 tctaagttca tttttttcta actttaattg ttcttcatat actgttttac tttcttcata
    24421 agatttttga gctaggtcta aagcattatt cttatctttt tccaaatttt ttaattctat
    24481 taattcttgg tttatattgt tgtatacttc ctctgtaatt ccatcatatt gacttaattt
    24541 tgatttcaaa tcttttaaat tttgtaactg tagatttttt ctctttttca ctttttctcc
    24601 aagacttctt ccatatttct caagattaaa aattctctct aacatgtctc ttctatctgc
    24661 acgtgataat cttagaaact cattaaattt gtcttgtggt aaaactactg acctagtaaa
    24721 atcatttgcc gttaatccta caacctgcgc tactttttca tttacttcca caactttatc
    24781 agctaaaaca ttttgggtat tgtcattcaa tacttctatt aatctagcat tagaggtctt
    24841 tgttcctgtt ttacttctag caatggttct atctacaatg tatcttcttc tagtgttttt
    24901 gcttcctatc tcaaactcgt atgatataat tgccttatcg cacacactgt ttatatattc
    24961 ttttgtattt ctggatatat ttccatacat agcaatagtt attgcatcta agattgtaga
    25021 ttttccactt ccagtagttc caaatatccc aaacaaacct ctttcaatta atttttcaaa
    25081 gtctataact tgtttatcaa tatagctatt aagccctgta agttctaatc taattggtct
    25141 catctgctga ttcaccttct tcacttatta tatctaaaaa taaatccata agttctcctt
    25201 ttggctctac acctttacta aaagaataga actctctaaa caactctgcc atagattttt
    25261 cttttatatc tacactttct tgttcataac aagatgttat tattggtttt atttctatta
    25321 tgtcttttaa tagttccttc atttttttaa tttcattctg tgatattatc tcatcagtat
    25381 ttatttcaaa atatgaccaa atctctctat cttgattttg ctcacatata tctagtgctt
    25441 cttctattcc attacattta aatacttcta taggtttgta attattaaaa tatacatctt
    25501 caattattgg tttttcacca gcttttatat ccactatgta tgcccctttg gtatacgctc
    25561 tttcatcctt gctatattga agtggagaac cagaatagta tgcatttaga cgttcggaag
    25621 ctttttgttg tttatgtaaa tgacctagtg cagtatattg agccttttct ggcaaatctt
    25681 ttctttcaac taaaaaactt ccacctaatt gtataggtct ttctgattca gtactctctc
    25741 cacctattac aaatatatgt gatacggcta tatttatagt atcacttcta aaattttctt
    25801 ctaaacttct aaatatatct cctacctttt cagaataatt tttttgtttc tcagaatcat
    25861 cttcacttga aaaaacttca tttaatcttt tttcacttgg ataaggtaat gtagcaacca
    25921 caatattttc tccatttata ttcagttttg tacatccctg agttgcatat gttatttcaa
    25981 accctttata cttagcttct atcgtagcac ttaatggata accatatata agtatccctt
    26041 gttcatgtgc tagaggcgtt attgcagaaa gtctctctgg attatcatga tttcctgaaa
    26101 ttattaatac acacctctga ccattattag caagtcttga tacagtctga taaaatagtt
    26161 tttctgcttg tgctggggga ttagaagtat catatacatc tccagcaatt ataaccatat
    26221 ctatttcatt tgattctact atttttataa attcctcaca aaactttgct tgttcctcaa
    26281 ttctagaatg gccttctagg gatttcccta gatgccaatc tgatgtatga ataaatctca
    26341 ttttttcccc ttcctctatc ttttaggttt aatttatata aaaaattaat aacaaacagc
    26401 ttcatcaact ccaaataaat atatacattt ctcttttact gacttaccag ttaaagtttc
    26461 aataatatct gcatataaat ccaactgttt tttatatttc tctattattt gatttatatt
    26521 ttcctcattt acaaaatctg ttttataatc caccaaaact atttgattat cttcttcaaa
    26581 ataagcatca actatacctc ttaacatgac actttcatta tcataaagtt ttttatcatc
    26641 attgtttata agcttttcat aaatatatat gtctttcata tttacttgtg cataaattga
    26701 tttttctcta tttataattt cagcattcaa cattcttttt cctatgttag atgcaaaaaa
    26761 tttatatatt ttatatggat ttactatact agcttgtttt tctgttatta tgccttttga
    26821 aacaaaacct cttatttgtg attttatatc atttacacta cttacatttt ttaagtctaa
    26881 aacctccata actaaatgga ctattgtgcc tctttctgtt ccacttattt tttctctttc
    26941 ttcttcattt tgaataaata gaggcttttt taatattact ttttgttcaa atatagtatt
    27001 tattaactct tcttcataat tattttgtat cttttttatt tctgtaacag atatagtagc
    27061 aggttttctt gtactaaatt catatggata tatatagttt aatttttctt ctatttcatt
    27121 gtaataatta ctgtcaggac tatctacatc tattttttca agaatttctt caatactttc
    27181 tttttcttca tcactcttct tttccactaa aatatcattc ttattccaca acttcccata
    27241 ccattttgaa ttatgttcaa cactaaaaac tgcgtctaat cctacttcct ctagtagatt
    27301 agataaatcc ctatgtttta atacacaagg cattatccaa tctaaaaaat ttttaccttt
    27361 taatatctcg tattgtgata ttgtatctaa actttcaact ccatttgacc atctctttat
    27421 actatcttgt atatttctag tagaacctgt tataatcaat ttttctttag ctcttgtaaa
    27481 tgcaacatat aaaactctca tctcttctga taaattttct atatttattt tgctttttaa
    27541 tgcttctttg gctatacttg gaaaagaaat ccttctctca tagtctacaa attgaggtcc
    27601 ataccctaaa ttatgatgat ataaaatact ctttttaaaa tcttgagtat tgaaattttt
    27661 acccattgca gagcatatga ctactggaaa ttctagccct ttacttttat gaatactcat
    27721 tattctaaca acatttgcat tctcaccaag tgtctttgca cttcccatat cagaactagc
    27781 tttttttaat ttctctataa aatttacaaa gttgaatatt cctttaagac tagtttcttc
    27841 aaattgtttt gctctctcaa acaatacttt taaattagct tgtctttgac ttccacctgg
    27901 caatgctcca acataagcat agtatccagt ccttgtgtac aaataccata taaattcatc
    27961 tgtactcatg tacattgact tttctttaaa ttcctttagc ttaattaaaa aatctttact
    28021 tttgtttatg cactcttcac ttggtataaa ctcagactca ttttcatttt gagaatctgt
    28081 aaatccatca tactctgctg tactttttaa aacctcataa aaaattttat ctttactctg
    28141 aactctaata tcaatcaaat cctctggtgt aaatccaaat ataggtgatt ttagtacaga
    28201 tataagaggt atatcttgca ttggattatc tattatctga agtaatgaca atattgtttt
    28261 tatttcaatc gtatcaaaat accccactcc aacatcagca tatgttggta tatccatatt
    28321 catcaattca tctgcaaaca ctggagccca tgcagatgta gctcttaaaa gaataactat
    28381 atctctaaat tccacaggtc tatatccatc tattccctta tcatagacct tttgaatttt
    28441 tccatcttca ttgactttca ttaaatcttt gataatgttt ccaaccattc tagcctcaag
    28501 ttgtatatta tctaattttt cttcttcctc tatttcattt tctttgttat taattctatc
    28561 gtctttatca tttataatat catcatcaag tttattatct ttttgtatta ggtgtatttc
    28621 tgttgctcca ccaataatag atttctcgtc tgtatccaca ttaaaatttg cacctaaatt
    28681 tagtctttct ttttctgtat attcaatttc tcctatattc tcattcatta tattttcaaa
    28741 tatataattt acagcatcta caacttcttc tctacttcta aagtttttgt aaagcattat
    28801 ttttctatgg gaacttccct ttttatcatt atagttatta tatttttgca aaaataattc
    28861 tggctttgct tgtctaaatc tatatatact ctgttttaca tcaccaacca taaatctatt
    28921 tggagtttct gtatttgcaa cagctttaag cagtacttcc tgcactagat tactatcctg
    28981 atattcatct ataaatattt cataaaactt gtttctatat ccaactgcta tatcagaagg
    29041 tataatatta cctttttcat ctacatctgt aagtatattt aatgcaaaat gctctatatc
    29101 gttaaaatct attattcctt tttctctttt cttattgcta tattcctctt caaatctcag
    29161 tacaacatta gatattggtt taactatgtt ataaagatat tttatttctt ctctaataga
    29221 atcattatct ttattgaatg tagcacttac tatactttct aaagacttct tagtcttatc
    29281 tcttatagtt tttgcctttt cttttgattc ttttatatac gaaggagcat cttttgaaat
    29341 tctttttact ccttttacat aattttcaaa agacatactt gccatctttt tatatgcctc
    29401 atcccaagat ttattacatg cctgtgaaat gtcagcaatt tttttatatt caacagataa
    29461 cttttctgca aaagtctcta attcttctat tgattccact tcttttaatg ccctttccat
    29521 atttagacaa agaccattaa tctctatttt tacagtgtct aatatagctc tagcccaaat
    29581 agactgtgaa aaatcaaaat tttcatcaat attaaatctt tctgctgagt caatcaacca
    29641 ttttttaggt tcaggagaag ccattgcaaa tgaatatatt cccaagataa tgtcttgtac
    29701 ttctttatct cctcctcttt ctgcataact ttcaaccaaa tttaaaaaac cctcatctct
    29761 ttcttcgtat aaatcttcaa atacttcctc tattgcttct tgtttcaaaa tagcacattc
    29821 agtttggtct cctattctga agttagggtc taagcttact ctatgaaagt ttgattttat
    29881 aacatccaaa cagaatgagt gtattgttgt aatactagat ttatttaata aaacaagttg
    29941 gttttgtaaa tgtttatttt ctgggttttc atctaaggct tttcctatag catcccctat
    30001 cctctctctc atttctgacg cagcagcatt agtaaatgta acaactaata acttatctat
    30061 atcaattgga ttttctctac ttgttatcat ttgaattata cgctcaacta gcacggctgt
    30121 ttttcctgaa ccagcagcag cagcaaccaa taaattgcat tctctacttt ctataacttc
    30181 taattgctct tttgtccatt taggagaact cactaattaa catctccttt catcattttt
    30241 attatttctt cattgctctt attatttaaa tttttatact tgttatcttt tatagttgaa
    30301 tcaaactgac atatagcaga gtaatcacaa aagtcacatg atgttccatc tttatgttta
    30361 tatggagcta tacgtatttc tccacttaac atttcttcac ataaatcctt tatagcatgc
    30421 cttacatatt ttctaagcag tttgaaatcc tcatagctta ttgctgaagt acttttacct
    30481 acatttccat ccttagtaag acctactggt attactaaag agtttttcct ttctccatct
    30541 attaatgatt tatccatctc tttaactatg tgagagtctt ttactacaag tcccttcatt
    30601 ctaagttctt ttaatattgc ttcttgtatt tcttcatcat ctttatcttc attaaatttt
    30661 gctattggat tatttattct acaatatagt attgcagctg gatttatatt tatatcttca
    30721 tcctttgcac tttcaaggat tgcatctaaa tacacaagta attgtaactg tagaccataa
    30781 tatatttctg ttagacttat ggacttattg cctgacttat aatctactat tcttatatat
    30841 ttattttctc cttcttcaaa ttcatcaact ctatctattt gtccaattaa acttacttct
    30901 tgtccatttt ctaacactat ttttataggg gaatattttc cattgtcgcc aaatttaact
    30961 tcataatcta ttggttcaaa tgaaccttgt tttatttgct gactaattat agttatagca
    31021 gttgtcaaca tattctttaa tctatatgca aggtatttat acctctcaga actatttaat
    31081 atatatcctg gtatctttaa aattatttca tcaactatta tacctatctt taattctata
    31141 tatttttcat caatatctct ccaagataaa ttatcttgta aaagttcttt tgaaaatcta
    31201 tctaaaatat tatgaataaa agttcctaaa tctggtggtg taaatgaata ctctttacgc
    31261 tttcttgctt ttagaccata ttgtataaaa taagcaaatg gacattcagc atatttttca
    31321 agcctagata tactaagaga attactttta tacaaagacc ttattttttt ctcttcaatc
    31381 ttatgaactt gattagtata actaaggcct tttataactt tttttgttat agaactataa
    31441 atttcatctt ttaaatagta cctatatata tctaaccaaa tactattaat ctcttctgta
    31501 ttgtaaccat cactgtcata attttttatt acgtttataa gttcattaaa tgtaggggat
    31561 ttaaccgtta tttttttaag tatatcttca tcagtgcttt tattttcttc cactaaataa
    31621 cttttatttt ctatatttgg aaatatcttc ttaagtcttg atataattat tgatggtcta
    31681 agtgttttac cttcatggtc tgatattgga tacgttatgg ttaaattctt agatgtcgaa
    31741 gtaagagctt tatatacaag aaactgttct tcaaaactcc tagtcttact atcaatatca
    31801 acttcgattc ctttgtttcc aagactttcc ctatcattat cactcaatat tccactatct
    31861 tttgtaatca gtggaaacac accatcagta gttccaacta ggtataaata tttcgtgtct
    31921 ggatttttca ttctatccac actacttaca agtacctggt ctatactagg aggaaccagt
    31981 cctaattcat attcatcaaa acctaaggtt attaatttta taaatttttc taaagaaatt
    32041 atttcatcac ccataagttc caccatttgg tctaatatgt ccactactat gtcccaaact
    32101 tgtgagtatt gatttgcaac atcaagttcc cctttatcct taaagtttac tattaaactt
    32161 tctatagttt ctggcatatt tatatctaat aaaaattcat atatatatct acagatttct
    32221 tttactctat tttttcctct taatttttct tgtaaaatta ctataggctc taatacttta
    32281 ttttttattt catttatttt ctcttttaat tccagttcaa attcactttc tttacctgaa
    32341 agactttgtg ttattctata atcccatttt tcatcaaacc attttttacc tttaatccca
    32401 ttagccaaca cataattttc tagtaaattt atatcatcat tatcaattcc tataagtcca
    32461 ctttttaaat acctaaacat agtttcatac ccatatcgtc tattcttcat ttctaaagct
    32521 gaaataatta aaataactat tggattactt tttgcctctc tcttcttatc tataaagttt
    32581 ggtatattat attcattaaa tatagagtgt actaaaaaat catatctact taaatctctt
    32641 gtagcaatgg ttatatctct atatctgaca ttcttatctc taactaaatg tacaatttct
    32701 ctggctatct cttccacctc tgaatataaa ttgttaaatt cttttatctt tatattttca
    32761 gtttgatttg aatatatttt atacggataa gcattataaa atctttctag gtgctgtaat
    32821 tccttaactt tttcaaatct ttttataaca cctgtattta aatttacttg tggcaatatt
    32881 tttattcctt cttcattgca caattgagtc aattttgaat aagtaaattt agttctggaa
    32941 aacatatcag atttactgta cccaatatag ttgatatcat ctacagtcaa agagatatta
    33001 acacttttag atttatttaa tatactcttt attacattgt actgatttgg tgtaaaacct
    33061 gtaaattcat ctatataaac acaagctcca tctaaataac tgcttaattc aattttactt
    33121 gccaatgaag ttaacatatc ttgagcatct acatagtttt catgcaattt cccttcaaaa
    33181 gaattatata ttttacttat atcttttaat tttaaactta aagtttcatt atccaattct
    33241 ccagatatat tttctaacat ttcaggactt atattgtatt gtttcatttc tgatatcata
    33301 tctgtaattg agcttacaaa accagactgc gattttgatt ttgaaaacac atttaattct
    33361 tcacttacat cttcaatagc tctatatacc atcattgctt ttccactaga atttatattt
    33421 acatctgtca atcctcctac ttgagaaaaa actatattac ttagtgtctt aaagctaagt
    33481 accctaactc ttaaatactt atctttttct tttcctaaaa atagtttact tactctattt
    33541 tcaacctcaa atgtgtattg ttctggaact aaaagtataa ttgatgtcgt ttcatttttt
    33601 tgagcttctt ttttaatctc atctagtata taggtggatt ttccacttcc acttctacct
    33661 aatacaaacc taagtcccat aaatttccgc ctccaaaata ctataatcta tttctttgcc
    33721 attacataac ttattaaatt cacaatactt gcaatgcttt ttatttctat agcacatacg
    33781 ttcttcttta ttatatacct tttctatatc attgtataga ttgtcttcgt atgtatgttc
    33841 ttcataatta gtgtttttta tcatacttat ataattttga attttatttc tatttgcttc
    33901 atgtttttct tcatcatata aaatggtaat tggtaagtca tcaaattcag gttgataata
    33961 tctcatattt atatctttaa catttatgtc catttttaaa actttaggta ttacttcttt
    34021 tgctaaaaac atataaacaa tagtttgtat tctattttct acgtgtttat agtcaatctt
    34081 tttagactcg gttttccaat cccatatttc tatattgtta tttccaatta caactaaatc
    34141 atactttgcc tgtattatat ccccatttaa ggtcatcctt acttcatatt ctggcaaata
    34201 cgtttttcct ttttcaatag gaacaacttt ttttatattt tccatccact ttaaaaactt
    34261 tttactattt ttatcatcct cattaaaata gattccaaga ggtatattgc taaaatatct
    34321 ttcacaaatc aaatgaaaat ctcttcctgt ttttaaagtt tcataatact ctctactacc
    34381 aacatcatca tttttccaat ttattctatc aatatattta tatttaaatt tcaatggaca
    34441 tgatttatat gtgttaattg aattttgact ataagaaaaa tattttaatt tatcgttcac
    34501 tcatatcact tctctcttaa ctttattttt tagtagttac ttactgtaca tattgtttta
    34561 tcccctttta gatatatttc aagcaatctt ttaataactt attatatatt aatttgacct
    34621 tttttcaata atatgttgat taagtctgtt taaatatata catggttttt gctcttttct
    34681 cttattccct atatcacttt catctctata agcacttcct gaaagtacta acatttcctt
    34741 tgctctagta atccctacat ataaaagtct aatcttttca tttatagaat caatcttcgt
    34801 tttatgagta taatctgttg aaatacttcc ttttaatata gcttcaactt ctgatttaat
    34861 aattgccata gggtttttat atttttcttt tagataccat ttatcactct gaaacttttg
    34921 attaatattg tcagggaaat tatattcaac aagacctagt aaaaacacac aatcccattc
    34981 catccccttt gacttatgat agttacatag tgttatacta ccgggctctg gttcatatcc
    35041 atttatctca tataccacat ctattatatg attaaacacc ttatttttta catcaaacaa
    35101 taaattatat acatcttcta aatccatatt tatattttct gatactagat actttatata
    35161 aaaagatata taatccagta cagctttatc ttctttttct agattaagtt ttttcccaat
    35221 aaacaaaact aagaggtcta accttattat tggatattct aatatctgtt ttataatatc
    35281 tattcctctt ttaaaaccta aacatatatc actttcttca ttaaattcta ttaaaccatt
    35341 atctaactta ctaaattcaa aattataaat aatttcttcg gtagaatagt tttttaattc
    35401 ttctaaaaaa tactttattt cttcttcatt atcagtatct ataaaaactt tattaagtac
    35461 aactattaac ttttctacat catcacaatg taacataaaa tcaataatat atgctatgtt
    35521 gtttataatc ttccttttat ttaaagaatt tggtcctaat tcttcaaagg ttagattatt
    35581 ctctctaagt tctttagcaa cttgtgtcac ttgttcatta aatggaacca atatacctat
    35641 acttttatct ggatatttat tttttatacc cttaacgtat ctagcagttt gctctatttc
    35701 aattttccaa ctctcatacc actttacatt tatattataa tgttctggat taggattttc
    35761 tttatatccc attccatttg gcacagtttt tatttgcata tcttcaagag catgtctaca
    35821 ttctttctgt ctaaattcct ttgtaacata cttgacaaga gtgtttgcta aatcaagtat
    35881 atcttttgaa cttcgatttg acatatccat cctataacag aaatctgcac tatttataaa
    35941 ttctttaaaa aatttagggt ctgatgatga aaatgttcct gttatactct gatttatatc
    36001 tccaactcta accaagtttt tattttcgct agatataatt tttattatat taccttgaat
    36061 ttcatttgag tcctgacact catcttcaaa tatatatttg tatctagctt gaaacttctt
    36121 tctaagccct tcatctaagc ttaaagtttt ttctgctaaa attaatatat catcatagtc
    36181 tagcaatcca ttttgcttca attttctatc atacaattta tatattggta aaataatttt
    36241 aagcattcct ttgtgtcctc ttgacaatat ttcttcaagt ttatctggag aaattccctt
    36301 atacttaagt tcacttattg catttcctgc aaattcaaaa aaacctcttt cccatgcttc
    36361 aagagttatt tctttccagc gttcatcttt ttgctctttt aaaaaccatc taaaagctct
    36421 ttctcctcct tcaaatctaa atctacttat acattcattt aatataatac ttttttgcaa
    36481 atcgtcagct atgttaaaat cctcacttaa cattacaact tctggctttt cctttattat
    36541 ttttacagcc aaactgtgta tagtcattac ttcatacgaa ttagtatcat ctattttatt
    36601 ctcttcaaga atagttctta ttctaccttt aaaattattt acagcactat tcatatatgt
    36661 aagaactaat attttaccac ccttatgctt ctgttcaagt aatagtttag ctactaaatt
    36721 tgttattata aatgtttttc cagcacctgg tactgctggt actgccatag taccattatc
    36781 gtaatttatt attggtatct ggtcttctct ataatttata ttaatcataa tttttatctc
    36841 ctttatgtga gatttttaaa agtatactat ataacatact ctcttggata taaccattaa
    36901 tcgcatattc acttttatat gcatagattt tctttgc
//
LOCUS       DAEMAE010000012        36468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEMAE010000012 DAEMAE010000000
VERSION     DAEMAE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.012"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..326
                     /locus_tag="KPH93_000521"
     CDS             <1..326
                     /locus_tag="KPH93_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687358.1"
                     /translation="YGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRI
                     REFYNKKIYLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKF
                     ENKNI"
     gene            498..1148
                     /locus_tag="KPH93_000522"
     CDS             498..1148
                     /locus_tag="KPH93_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG1687359.1"
                     /translation="MLLDKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSNKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            1324..2256
                     /locus_tag="KPH93_000523"
     CDS             1324..2256
                     /locus_tag="KPH93_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG1687360.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNLI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            2530..3243
                     /gene="ispD"
                     /locus_tag="KPH93_000524"
     CDS             2530..3243
                     /gene="ispD"
                     /locus_tag="KPH93_000524"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG1687361.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFNENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            3240..3725
                     /locus_tag="KPH93_000525"
     CDS             3240..3725
                     /locus_tag="KPH93_000525"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG1687362.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    3820..4028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4092..5810
                     /locus_tag="KPH93_000526"
     CDS             4092..5810
                     /locus_tag="KPH93_000526"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG1687363.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDADDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIIPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLGQ"
     gene            5928..7373
                     /locus_tag="KPH93_000527"
     CDS             5928..7373
                     /locus_tag="KPH93_000527"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG1687364.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            7814..9295
                     /locus_tag="KPH93_000528"
     CDS             7814..9295
                     /locus_tag="KPH93_000528"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG1687365.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMKELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATSKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGSEFLRLEHIPTLIDALREKIEKAEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            9516..9671
                     /locus_tag="KPH93_000529"
     CDS             9516..9671
                     /locus_tag="KPH93_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687366.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            9816..11213
                     /locus_tag="KPH93_000530"
     CDS             9816..11213
                     /locus_tag="KPH93_000530"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG1687367.1"
                     /translation="MKVYNTLTRAKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEIELVKELDNFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            11198..11611
                     /locus_tag="KPH93_000531"
     CDS             11198..11611
                     /locus_tag="KPH93_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG1687368.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQKK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            11717..12475
                     /locus_tag="KPH93_000532"
     CDS             11717..12475
                     /locus_tag="KPH93_000532"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG1687369.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            12488..13225
                     /gene="rlmB"
                     /locus_tag="KPH93_000533"
     CDS             12488..13225
                     /gene="rlmB"
                     /locus_tag="KPH93_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG1687370.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            13228..13755
                     /locus_tag="KPH93_000534"
     CDS             13228..13755
                     /locus_tag="KPH93_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG1687371.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDY
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DEETLSKLENIRRKR"
     gene            13820..14467
                     /gene="sigH"
                     /locus_tag="KPH93_000535"
     CDS             13820..14467
                     /gene="sigH"
                     /locus_tag="KPH93_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG1687372.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            14541..15734
                     /gene="tuf"
                     /locus_tag="KPH93_000536"
     CDS             14541..15734
                     /gene="tuf"
                     /locus_tag="KPH93_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG1687373.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVV
                     ATIIE"
     gene            16105..16416
                     /gene="rpsJ"
                     /locus_tag="KPH93_000537"
     CDS             16105..16416
                     /gene="rpsJ"
                     /locus_tag="KPH93_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG1687374.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            16508..17137
                     /gene="rplC"
                     /locus_tag="KPH93_000538"
     CDS             16508..17137
                     /gene="rplC"
                     /locus_tag="KPH93_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG1687375.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            17167..17790
                     /gene="rplD"
                     /locus_tag="KPH93_000539"
     CDS             17167..17790
                     /gene="rplD"
                     /locus_tag="KPH93_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG1687376.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            17790..18080
                     /gene="rplW"
                     /locus_tag="KPH93_000540"
     CDS             17790..18080
                     /gene="rplW"
                     /locus_tag="KPH93_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG1687377.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            18110..18940
                     /gene="rplB"
                     /locus_tag="KPH93_000541"
     CDS             18110..18940
                     /gene="rplB"
                     /locus_tag="KPH93_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG1687378.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            18975..19256
                     /gene="rpsS"
                     /locus_tag="KPH93_000542"
     CDS             18975..19256
                     /gene="rpsS"
                     /locus_tag="KPH93_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG1687379.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            19288..19623
                     /gene="rplV"
                     /locus_tag="KPH93_000543"
     CDS             19288..19623
                     /gene="rplV"
                     /locus_tag="KPH93_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG1687380.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            19646..20461
                     /gene="rpsC"
                     /locus_tag="KPH93_000544"
     CDS             19646..20461
                     /gene="rpsC"
                     /locus_tag="KPH93_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG1687381.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            20497..20928
                     /gene="rplP"
                     /locus_tag="KPH93_000545"
     CDS             20497..20928
                     /gene="rplP"
                     /locus_tag="KPH93_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG1687382.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            20930..21133
                     /gene="rpmC"
                     /locus_tag="KPH93_000546"
     CDS             20930..21133
                     /gene="rpmC"
                     /locus_tag="KPH93_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG1687383.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            21157..21411
                     /gene="rpsQ"
                     /locus_tag="KPH93_000547"
     CDS             21157..21411
                     /gene="rpsQ"
                     /locus_tag="KPH93_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG1687384.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            21437..21805
                     /gene="rplN"
                     /locus_tag="KPH93_000548"
     CDS             21437..21805
                     /gene="rplN"
                     /locus_tag="KPH93_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG1687385.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            21829..22134
                     /gene="rplX"
                     /locus_tag="KPH93_000549"
     CDS             21829..22134
                     /gene="rplX"
                     /locus_tag="KPH93_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG1687386.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            22164..22706
                     /gene="rplE"
                     /locus_tag="KPH93_000550"
     CDS             22164..22706
                     /gene="rplE"
                     /locus_tag="KPH93_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG1687387.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEIISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            22724..22909
                     /locus_tag="KPH93_000551"
     CDS             22724..22909
                     /locus_tag="KPH93_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG1687388.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            22942..23340
                     /gene="rpsH"
                     /locus_tag="KPH93_000552"
     CDS             22942..23340
                     /gene="rpsH"
                     /locus_tag="KPH93_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG1687389.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            23371..23913
                     /gene="rplF"
                     /locus_tag="KPH93_000553"
     CDS             23371..23913
                     /gene="rplF"
                     /locus_tag="KPH93_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG1687390.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTNNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            23949..24317
                     /gene="rplR"
                     /locus_tag="KPH93_000554"
     CDS             23949..24317
                     /gene="rplR"
                     /locus_tag="KPH93_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG1687391.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            24338..24847
                     /gene="rpsE"
                     /locus_tag="KPH93_000555"
     CDS             24338..24847
                     /gene="rpsE"
                     /locus_tag="KPH93_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG1687392.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            24862..25047
                     /gene="rpmD"
                     /locus_tag="KPH93_000556"
     CDS             24862..25047
                     /gene="rpmD"
                     /locus_tag="KPH93_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG1687393.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            25080..25523
                     /gene="rplO"
                     /locus_tag="KPH93_000557"
     CDS             25080..25523
                     /gene="rplO"
                     /locus_tag="KPH93_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG1687394.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            25567..26835
                     /gene="secY"
                     /locus_tag="KPH93_000558"
     CDS             25567..26835
                     /gene="secY"
                     /locus_tag="KPH93_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG1687395.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            26853..27503
                     /locus_tag="KPH93_000559"
     CDS             26853..27503
                     /locus_tag="KPH93_000559"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG1687396.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKD
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            27504..28250
                     /gene="map"
                     /locus_tag="KPH93_000560"
     CDS             27504..28250
                     /gene="map"
                     /locus_tag="KPH93_000560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG1687397.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            28272..28541
                     /locus_tag="KPH93_000561"
     CDS             28272..28541
                     /locus_tag="KPH93_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HBG1687398.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVRHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            28579..28797
                     /gene="infA"
                     /locus_tag="KPH93_000562"
     CDS             28579..28797
                     /gene="infA"
                     /locus_tag="KPH93_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG1687399.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            28833..28946
                     /gene="rpmJ"
                     /locus_tag="KPH93_000563"
     CDS             28833..28946
                     /gene="rpmJ"
                     /locus_tag="KPH93_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG1687400.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            29099..29470
                     /gene="rpsM"
                     /locus_tag="KPH93_000564"
     CDS             29099..29470
                     /gene="rpsM"
                     /locus_tag="KPH93_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG1687401.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            29506..29904
                     /gene="rpsK"
                     /locus_tag="KPH93_000565"
     CDS             29506..29904
                     /gene="rpsK"
                     /locus_tag="KPH93_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG1687402.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            29936..30559
                     /gene="rpsD"
                     /locus_tag="KPH93_000566"
     CDS             29936..30559
                     /gene="rpsD"
                     /locus_tag="KPH93_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG1687403.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            30637..31584
                     /locus_tag="KPH93_000567"
     CDS             30637..31584
                     /locus_tag="KPH93_000567"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG1687404.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            31605..31946
                     /gene="rplQ"
                     /locus_tag="KPH93_000568"
     CDS             31605..31946
                     /gene="rplQ"
                     /locus_tag="KPH93_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG1687405.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            32066..32899
                     /locus_tag="KPH93_000569"
     CDS             32066..32899
                     /locus_tag="KPH93_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG1687406.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDDLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDGMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            32887..33753
                     /locus_tag="KPH93_000570"
     CDS             32887..33753
                     /locus_tag="KPH93_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG1687407.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLEYEEFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            33747..34550
                     /locus_tag="KPH93_000571"
     CDS             33747..34550
                     /locus_tag="KPH93_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG1687408.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            34582..35313
                     /gene="truA"
                     /locus_tag="KPH93_000572"
     CDS             34582..35313
                     /gene="truA"
                     /locus_tag="KPH93_000572"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG1687409.1"
                     /translation="MRNIKITIQYKGKNYCGWQKQPDSLGIQGTIERAIYDITKEETN
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            35433..35864
                     /gene="rplM"
                     /locus_tag="KPH93_000573"
     CDS             35433..35864
                     /gene="rplM"
                     /locus_tag="KPH93_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG1687410.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            35893..36285
                     /gene="rpsI"
                     /locus_tag="KPH93_000574"
     CDS             35893..36285
                     /gene="rpsI"
                     /locus_tag="KPH93_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG1687411.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT        14826 a         4092 c         7343 g        10207 t
ORIGIN      
        1 cttatggtat attcaatact tttatatcat caagtaatcc taaaaaaatt attattgata
       61 gcaaatgtat acattttacc tcatttatga ctacacataa gtatgaaatt aaagatatag
      121 aaaagataag aataagagaa ttttataata aaaaaattta tctgaaaatt aatgatggaa
      181 acctttttaa aggtaggtat tggataagaa caaatatgtt taataatagt atagcattat
      241 atagctattt cattgagttg gaagagtgct tatatccaga ttcactaaaa tttagaaata
      301 aaaaatttga gaataagaac atataaaaat aaaaaattga attgaagcct agcttaatct
      361 tttttgataa gatgtaagtt tgagttttat actaaatttt tagaattttc aatcaagaca
      421 ataacacaat atctaagcaa ttagaacaca attaagattt ataaggcatg tatatgatga
      481 atgggaggat ttaattaatg ttattagaca aattaagaaa agaagtatta caagcatctt
      541 tagacctttt aaactataat ttagtgacat taacaggagg aaatgtaagt gggagagatg
      601 aacaaactgg ctatatagca atcaccccaa gtggaatgga ttatagaaat cttacaccaa
      661 gtgatattgt tatagtagat gtagatggaa atattataga tggaaaatgg aaagcatcag
      721 tggatttatc agaccatttg tatatatata agcatagaga agacataaat agtataattc
      781 atacacattc tacatactca agttgttttg ctattttaaa tgaacctata gaatgtgcaa
      841 gtacaactct tgcaaatgaa gtaggaggtt cagtaccagt tgcaaaattt tcaccaccaa
      901 catctaataa gatgggaaaa tgtgttattg aagcaattgg tgacaaaaga gcatgtcttt
      961 tagcaaatca tggcgtaatt gcagttggtc catctgtagg acatgctctt acagccgctg
     1021 taatgttaga agattcagca aaagtatatt acttagcaaa atcaattggc acaccagtgc
     1081 tactgcctga tgaagaaatt caaagagcga gagatgtatt ttttaatgtt tatggtcagg
     1141 ataaataaat tagataaagt tttaaaagta atatgaaaat atagatattc aatgatatta
     1201 aggagattta taatgatata ataagtttaa tagttaagaa gtaaaatatg aaatgtaata
     1261 gtacataaat tcatttaaga ctaaaaaata ataagataaa ataaaaaagg agtctgtatt
     1321 tctatgtatt acttattaaa caaaagcaca aatccttact ttaatttagc attagaagaa
     1381 tatctatttt taaatgataa atataatgat gatattataa tcatatggag aaatgaagaa
     1441 tccattttta taggtaaaaa tcaaaatcca tatcaggaag tacatcatga tgttattgaa
     1501 aagggagaaa ttcctatttt aagaagaata tctgggggag gcactgttta tcatgactta
     1561 ggtaatatca atatgtcgtt catacaaaaa gataggcaac ttcatgagat agattttttg
     1621 gagcatacta agtttatgca aaagatgctt tcaaccttag gactagatgt atctataact
     1681 gaaagaaaag atttattttt aaatgggaaa aaaatttcag gttctgcaca gtctataaaa
     1741 agaaaaaatt ctctttatca tggaactctt ctttacgatt cagatttaaa taaattaact
     1801 aaatatttaa attcaaataa agcaactgaa tcaaatgcaa ctaaatctgt ttcaagtaaa
     1861 gttacaaata ttaagttttt actcgaaaaa gatataaatt actttttaga ttattgtgtt
     1921 gaatatttaa aatctaatat aaagaatata aaagaacttg aattggatga agaagatatt
     1981 aaaagtgtat acaatatagg agaagataaa tataaaaaga tagagtggac atatggtaaa
     2041 acgccgaaat ttcaagtagt gctaccattt aataatgaaa gtaaaataaa tatacatgtt
     2101 aacagatgga aagtatcaaa attttttatt tcttatgatg ataatttaat taatatggac
     2161 aagttgttag atttgaattt ctttaaagaa gaaataaaag agagtatttt agaaaattat
     2221 ccacattaca tagatttaat caatttaata ttttaatata aaacctctaa tttgataaaa
     2281 ttgtcttata tcaaactaga ggtttttaat attcttgcat attaacaacc attacaaaat
     2341 tgtaaaatta aaaacaacac tattgaaaaa tcaatatata ttgtaataaa tatacgtgtt
     2401 tttcattagc aaatagacat taactattta tacaactgaa aatttaatat ataattaatc
     2461 agatggttaa aataaaaata ttaaaagttt taataaatga tggttaaaaa taaaatacga
     2521 ggtgatacca tgtatagtgt tataattgtt gcggcgggaa gcggaaggag aatgaactta
     2581 gatataaaca aacagttcat aaaattaaga gaaaaagaaa ttattgctca tacaattcaa
     2641 gttttttatg aaaatataaa tatagatgaa atagtggtat gtataaaaaa agaagaagag
     2701 gattttttta atgaaaatat aataaataag tataacttta aaaatataaa aatagcatat
     2761 ggcggaaaag aaagacagga ctcaatatat aatggtttaa aaaaattaga taaaaattgt
     2821 gatatagtac ttatccatga tggagcaaga ccatttgtcg accatagaat aatcaatgaa
     2881 tctataaagg tagctaaaga aaaaaaggct gtggttgtag gtgttccagt aagtgatact
     2941 ataaagatag tgtcagatgg aacagtacaa gaaactccag aaagaaattt gttgtgggca
     3001 gcacaaaccc ctcaaacctt tgaatataat ctcataatag atgcttatga acaagcatat
     3061 aagaacaatt attatggtac tgatgattca atgttggtag aaaatatagg tcaaagtgtc
     3121 actatggtaa tgggttctta tgaaaacata aaaataacta gtccagaaga tttaaatata
     3181 gcagaacaaa tattaaatat ggaaaaaaga gatgaagtta acagtagaag gagaattata
     3241 tgagaatagg attaggatat gatgtacaca aattaacaga ggatagaaaa cttataatcg
     3301 gtggtgtaga aataccacac gataaaggtt tgttgggtca ttctgatgct gatgtattga
     3361 tacatgcaat aatggattct attttaggtg ctctagcatt aggagatata gggaagcatt
     3421 tcccagatac agatgaagaa tataagggag cagatagtat gaaattatta gaacatgtgt
     3481 ataatctaat aacaagtaaa ggatataaaa ttggtaatat agatagtact ataattgctc
     3541 aaagccctaa gatggctcca tatattgaga gtatgagaag taatatatca aaagttttaa
     3601 acacagacat tgataatata aacataaagg caactacaga agaaggtctt ggttttacag
     3661 gagcaaaaca gggaatagct tcacaaagta tttgtttatt attgttgaca agtcagaata
     3721 attaatatat tattagtaat aagtttaatg gatatcaaat aataggccta tatagaatat
     3781 ctataatgtg tgaatttaag tgaaattaat ataaatataa aattagctat gataaagaaa
     3841 tagtaaaatg atgaaatctt cacagagaga aaacgttggt gagagttttc aagaggaagt
     3901 ctttgaacct gtcttttagc ttaaaggtat aaaaatcttt acggtaggaa ccgttatatt
     3961 cccaaaagag agctatttat atagctaact agagtggtac cgcggaaatt aaacctttcg
     4021 tctctatact tgtatagaga tgagaggttt tttatatttt caggaagtta atattcagga
     4081 ggaatctatt aatgaaaatg tcaaaaatgt ttatgccgac attaaaagaa atcccagcag
     4141 atgctgagat tacaagccat caattaatgg tcaggtcagg catgataaaa aaaatgactt
     4201 caggtgttta taaccaatta ccaatggggt taagagtgtt caaaaaaatt gaacaaataa
     4261 ttagagaaga attaaataaa aaagattgtc aagaaatact ttgtgcagcc ttgcttcctt
     4321 ctgaattatg gaaagagtct ggaaggtgga ctgctatggg tgaagaaatg tttagactaa
     4381 aagacagaac agaaagagag tactgtcttg ggccaacaca tgaagaagca ttcactgata
     4441 taataagaca agaaattact tcatataaac aattgccact gaacttgtac caaatacaag
     4501 taaaatatag ggacgagaga agaccaagat ttggtgttat gagaactaag acttttacaa
     4561 tgaaggatgc ctatagtttt gatgcagatg ataaaggttt agataagtca tatcaagata
     4621 tgtttgatgc atatgtaagt atatttgata gatgtggatt ggagaatagc cctgtacagg
     4681 cagattcagg agctatagga ggttctacat cagccgaatt tatggttaag tcagaagttg
     4741 gtgaagatga agttgtattt tgtagtggat gtgattatgc agccaatgta gaaagagcag
     4801 aatcttgtaa tctagcatca caaaaagaag aaatgaaaga attagaagag gttcatacac
     4861 caggagcagc tacaataaaa gagttagaag aatttttaaa aacttctcca gacaagtttg
     4921 ctaaaacttt agtatatgag gcagatggaa aaacagttgt agttgttgta agaggagata
     4981 gagaagttaa tgaaatcaaa gtatctaatg ctataggttc tgttatagaa tttgctcttg
     5041 ctacagatga tgtagtgaga aaagtaacta atgcagaagt aggatttgca ggtccaatag
     5101 gtataaatgc tgactatgta tttatagata aagaaattgt tgaacaaaga aacatagttg
     5161 ttggggctaa taaaactgag taccatataa aaaatgctaa ctatggaaga gattttgaag
     5221 gaatagttgg agattttaga aatgttcaag aaggcgacaa gtgtatagta tgtggtaagc
     5281 cacttgaaat agctagaggt gttgaagtag gacatatatt taaactagga actaagtact
     5341 cagaatctat gaatgcaaac ttcatagaca aagatggaaa atcaaagcct atagtaatgg
     5401 gatgttatgg aataggtgtt gaaagaacag cagcagctat aattgaacaa cacaatgatg
     5461 agaaaggtat aatatggcct ttgtcagtag ctccatacca tgttgtgata attccagcaa
     5521 atatgaaaaa tgaagaacaa atatcaatag ctgaaaatat atataatgat ttgcaagcta
     5581 tgggagtaga agttttatta gatgacagag atgaaagaat aggtgtcaaa ttcaatgatt
     5641 cagaattaat aggaatacct atgaggatta cagtaggtaa aaatattaat gaaggtaaag
     5701 tagaatttaa acttagacat aaagaagata aagagataat tgacatagaa gaaataaatg
     5761 aaaaagtaaa ggcagagttt atcaggaata atgttaggtt aggacaataa cttaaataag
     5821 atattgtata gataatcaga gttgtacttt atagacacca ttttatctat atgaaggata
     5881 taaaatggtg tttttttata aaacaaataa atgaggaggt aattgaaatg gcaaaaaatg
     5941 aaaaacaatt tgttgaagaa ataacaaaga tggaagatga ttttccacaa tggtatacag
     6001 atgtaataac taagactgac ttagttgatt atgcaccagt aaaaggtttt atggttgtaa
     6061 agccatatgg atatgcatta tgggaaaaga tgcaagaatt tatggataaa aaatttaaag
     6121 aaactggaca taaaaactgt tattttccat tattaatacc agagagttta ttaaacaaag
     6181 aagcagaaca cgttgaaggt tttgctccag aagttgcatg ggtaacacat gggggaaata
     6241 aaaaattaga agaaagatta tgtgtaagac ctacttcaga gactataata tgtacaatgt
     6301 atgcgaaatg gttaaaatca tatagagaat taccatatct ttataatcaa tggtgctcag
     6361 ttgttagatg ggaaaaatca actagacctt tcttaagaac atctgagttt ttatggcaag
     6421 aaggtcatac tttacatgaa acagcagaag aagcacaaga agaaacaata caacaattag
     6481 aagtatataa agcgctatgt gaagaattac ttgcaatgcc agttgtagct ggtcaaaaaa
     6541 gtgaaagtga gaaatttgca ggtggtgaaa gaacatatac tatagaagct atgatgcatg
     6601 atggtaaagc attacaatca ggtacaagtc atttcctagg tcaacacttt actaaggctt
     6661 ttgatattac ttttgcagat agagaaggaa acttagctaa tccataccat acatcttggg
     6721 gagcatctac aagacttata ggtggactta taatgactca tagtgataac agaggtttag
     6781 tattaccacc aagagtagct ccaattcaag ttgttatagt tcctatagct gctaaaaaag
     6841 gaaatgtaat ggagactgtt gataaaatat atgctgattt aaaaactaag ggagtagccg
     6901 tagaagttga tgatagagat aactatacaa ctggatggaa atttaatgag tgggaaatga
     6961 aaggtgttcc agtaagagtt gaaattggac caaaagatat agaaaataat caagctatgg
     7021 tatttagaag agatacttta gaaaaggatt ctatgccttt agaaggatta gctgatgcta
     7081 tatgtgattt atttgatgtt atacacaatg atatgtttga aaaagcaaga aaacatagag
     7141 aagacaatac atctatagtt gaaaatatgg atgagtttag aaaagcttta gaagaaaagc
     7201 caggattcat aaagactatg tggtgtggag atgcagaatg tgaagctaag attaaggaag
     7261 aaacaggagc aactataaga tgcttacctt ttgaacaaga aaacttaggt cacaagtgtg
     7321 tgtactgtgg aaaagaagca gatagtatgg tagtaatggc aaaagcttac taaaatacat
     7381 ttaaataaaa actggggtag aaagtattta atactttatc ccagtttttt tattttttat
     7441 gagcaataaa attgtatttg taactatgtc ataagataag tcatagatac ttatattaaa
     7501 aagaatataa tagagaacta tttttaataa ttacatatta atgaaagcaa aatatgtttg
     7561 ttatatagat tttcaataaa gcaaacaata aatataaatg aatattaata aagagtactt
     7621 ttaattttta gaaaataaat gacttaaaag aaagatttat gaaattaaaa ctaggatttt
     7681 ctgatataat aaacatgtgt atttttaaat aaaaaggata ttattattag atttgattaa
     7741 aaataaactt gcaaaacaat gtactagttt aatattatag ttatattgaa taaaatatca
     7801 ggaggaagta aaaatgaatg taagagtaag gtttgcacca agtccaacag gatttgtaca
     7861 tataggtagt ttgagaactg ctttatataa ctatttattt gcaaaaaaaa tgggcggaga
     7921 atatatatta agagtggaag atacagacca aagtagatta gtagaaggtg ctatagagaa
     7981 tatgcttaat gctatgaaat gggctggagt caaccatagt gaaggtgtta tattagatga
     8041 tagtggaaaa gtagtacaaa aaggagaata tggtccatat attcaatctc aaagattaga
     8101 tatataccaa gaatatataa agcaactttt agatagtgga aaagcttatt actgtttctg
     8161 tacaaaagaa agactggatg aagtaagaga tgctcaaaga gcagcaggag aaacagctaa
     8221 atatgatggt cattgtaaga atctttctaa agaagaagta gaagcaaata taaaagcagg
     8281 aataccatat gttataagat taagattgcc agaaaatcat acaattaagt ttacagactt
     8341 agttagagga gatatggaat ttaatactaa tgatttagat gaccaagtat taatgaaaac
     8401 agatggattt ccaacttatc attttgcagt agttgttgat gactatttga tgaaaattac
     8461 acatgttata agaggagaag aatgggtatc atctactcca aaacatgttt atttatatga
     8521 agcttttgga tgggaagcac ctgtatttgt gcatttacca aatatactta ataaagaaaa
     8581 gaaaaaatta agtaaaagac aaggtgatgt agctgttgaa gactttaaga aaaaaggata
     8641 tttaccagag ggattagtaa actatgtagc attagttggt tggtctccag aagataatca
     8701 agaattgttt acaatgaagg aattagaaaa agccttctct gtagaaagag tatctaaaag
     8761 tggcggagta tttgatactg aaaaattaaa ttgggttaat cagcactata taaaagatgg
     8821 tgatgatgct tatctaacag acttagcaat accattttta atagaagatg gatttataac
     8881 agaagaagaa gctactagca aatatgattt cttaaagagt atgatatcag tacttaaaga
     8941 gaaattacaa tatgtaaaag aagttactga acatgcaagt atattttttg gagataaaat
     9001 agaggtagaa actgaagagg gaagtgagtt cttaagatta gaacatatac ctactttaat
     9061 agatgcttta agagaaaaaa tagaaaaagc agaggtatta aatgctgagt tcgttcaagc
     9121 tatgcttaaa gaaatacaaa aagagtacaa aataaaaggg aaaaatttat ttatgggctc
     9181 aagaataatt ttaacagggc aaatgcatgg cccagattta ccaaaagtaa tggaagtttt
     9241 aggaaaagaa acttgcttaa atagaatagc ttatgtaaaa aataatatat tatagtcata
     9301 aaagtccgct aatttagaag cggacttttt ttgtgttagt atttggtgaa aatatctgct
     9361 taagaaaatg aaggtattta aggtaataaa tttaatatat aaaaagcaaa gcaaaaatat
     9421 aaataaaata tgaataataa actaataaaa tattaagcat agaagaaggg aatctattat
     9481 tttagtttga tttaattatt aagggggtaa ttactatgta tagtaatatt gatgatgtta
     9541 aaaaggaatt aaaggagcta tgcttagaat atgttacaat tttagaaaaa ttaaaggatg
     9601 aaaaaatgat tacagaagaa acttttgaaa agtgctcttc tcaaaaaaag atatttttag
     9661 aagaacaata gaggatggta atagtattat tattatgtag tttgaaagat agtacgaaaa
     9721 atgatagtaa tactattatt tattgcaatt ttcaactata atataatata taatgttaag
     9781 aaaataattt ataaaaatac agtgaggtga tgagtgtgaa agtttataat acattgacta
     9841 gagcaaaaga agaatttgtt ccattagaag aaggaaaggt aaaaatgtat gtgtgtggtc
     9901 caacagttta taactatata cacataggta atgcaagacc atttataata tttgatactc
     9961 taagaaggta tttagagtat agaggatatg atgtaactta tgtacaaaac tttacagatg
    10021 ttgatgataa gattataaat agaagtcatg aggagggaat atctcctgaa gaagttgcgg
    10081 ctaagtatat aaaagaatat tttgtagact gtgatgggct aggaataaaa agagctactg
    10141 ttcatcctca ggttacagat aatatacaac agattataga gtttataaaa gagttggaag
    10201 ataagggata tgcatatgca gtaaatggtg atgtatattt tgatacaaat aagtttgaag
    10261 gatatggaaa actttcaggt caaaaacaag aagatttaga agcaggggct agaattgagg
    10321 ttaatgacca aaaaagacat cctatggact ttgttctttg gaaggcaaaa aaagaaggtg
    10381 agccaggatg ggatagccct tggggagaag gtagaccagg atggcatata gaatgttctg
    10441 ttatgtctaa gagatattta ggtgaaacaa ttgatataca tgcaggtggg caagatttaa
    10501 cattcccaca tcatgaaaat gaaatagctc aaagtgaagc tagaagtgga aaaacattct
    10561 caaaatactg gatgcataat ggatatataa atataaatga tgaaaagatg agtaaatcaa
    10621 aaggtaattt ctttacagta agagatatat caaaattata tgatttagag atagtaagat
    10681 ttttcatgtt atcagcacat tatagaaatc cagtaaactt tagtgatgag atgttaaatc
    10741 aagctaaagc aggtcttgaa agattgtata atactaaaga aaaattagaa tttacactaa
    10801 gtaatttagt agaatcacct ttgacagaaa aagaaataga acttgttaaa gaattagata
    10861 actttagaca aaagttcata gatgctatgg atgatgatgt taatacagca gacgcagtaa
    10921 gtgtaatatt tgaattagct aagttaataa attctaatgt agatgaaaat tcttctttgg
    10981 agtttgctaa aaaatgctta gatgaattta atgaacttac aggagtgtta aatattgtaa
    11041 ataagaaaaa ggatactgta ttagataagg atattgaaga attgatacaa aaaagaactg
    11101 atgcaaagaa aaataaagag tttcaattag ctgatgatat tagacaacaa ttactagata
    11161 tgggcattgt attagaagac acaagacaag gtgttaagtg gaaaaggata taaaaatgga
    11221 aaaaaccgaa ttagttacta tgtcaccatt agttttagca tacttaggtg atacagtgta
    11281 tgagacatat ataagagaat atttaattag acagaataca caaaaaaaag ttaatgattt
    11341 gcataaatta gctataaaat atgtaaaagc taaagctcag gcaactataa ttcatgaaat
    11401 tgaaattgag ttaactgaag aagagagtaa aatttataaa aggggaagaa atcaaaagtc
    11461 aaatacttct cctaagaatg cagatattat agactacaaa catgcaactg ggtttgaggc
    11521 attagtggga tatttgtatt taaataatga aatagagaga cttcaatata ttatcaataa
    11581 aggaatcaaa attattgaaa gagatatgta aatattaaag agtgttgtct taataataag
    11641 taataaggca acactttttt tataactaat gtataataaa tatatagaaa ttagtaatga
    11701 gaaaggataa aaaattatga aagtaaaatt aatatcacat acacctgaac cagaaaaagt
    11761 aatagctatg gcagctaagc tatgttattc accagttggg acagacgaaa tagaaaaaga
    11821 cttaacagat gaaagtatag agaaattttt aaatatgtta ttaagtatag gtcatgggtc
    11881 aatattagaa catgcttcat ttacattttc aatagaaggt atttcaaggg cttgttcaca
    11941 tcaaattgtt agacatcgta tagctagttt ttcacaacaa agtcaaagat atgttaaatt
    12001 agaacaattt gagtacataa ttcctccaga aatagaaaaa atagaaaagg caaaagagtt
    12061 atttatagac tccatgaaaa aagaccaaga gaattatgat aagttagttg aaatattgtt
    12121 tgaaaatcat tataatgacc taataaaaaa cggtaaaaat gagaaaacag ctaaaagaca
    12181 agctgaaaag aaagcaatag aagatgctag atacgtattt ccaaatgcat gtgaaacaaa
    12241 aatggtattt actataaatg ctagaagttt gtttaatttc tttgaacata gatgttgtga
    12301 aagagcacag tgggaaataa gaaatttagc tgtggagatg ttaagagagg ttaaaaaggt
    12361 tgctcctata ttatttaaaa agactggtcc aagttgtgta aatggaagtt gtccagaagg
    12421 aactatgact tgtggagata tagttcaagt tagagaaaaa tttaaggcct tgtaggaggt
    12481 gaataatttg gcgagtatag aaggaagaaa tccggtaata gaagcaataa aaagtgacag
    12541 agaaatagat aaaatattaa ttgcaaattc agctaaagaa ggttcaatta agaaaataat
    12601 aggaatggct aaagataaga atataattat tcagtatgtt gataaacata agctagatga
    12661 agtaagtaca agtcattcac atcaaggtgt aatagcttat gcaagtgagt ataagtatta
    12721 tgaattagat gagctaatag atttagcaaa gaataaagat gaagacccat ttttcattat
    12781 attagatgag ataacagacc cacataattt gggttcaata ataaggacag cagatgcagt
    12841 aggagcgcat ggagttatca ttccaaaaag aaggtctgta cacataactc ctgtagttgc
    12901 caaagcatct gctggagcag tagaatatat gccagtttgc aaggtaacca atatagttaa
    12961 tacaataaaa agactcaaag aagaaggatt gtggattgct gcagctgata tggatggaga
    13021 aactttttat aaacaaaatc ttactggtcc tcttggtgta gtaattggaa gtgaaggttt
    13081 tggaatatct agattggtta aacaaaactg tgactttata gtaaaaatgc caatgatagg
    13141 taatgttaca tctttaaatg catctgtagc aggtggaata cttttatatg agatattcag
    13201 acagagactg gataaaagta aataggcatg aaaaaaaata taaatcatta cttaataata
    13261 gatggctaca atattataaa tgcgtgggac aatttgaaag aactggctaa agaagactta
    13321 gaagattctc gagaaaagct tattgatgac attatagaat tttctgagtt tatggggtat
    13381 aagactataa tagtttttga tgcttataat gttaaaaatt ctcgagaaaa agttgaaaaa
    13441 agaaaacaca taacaatagt ttatacaaga gaacaccaaa cagcagatag ttatatagag
    13501 aaatttataa cttctttatc aaagtatgat tatgtaaaag tagccactaa tgactatgca
    13561 gaacagcaaa tgattttagg aaaaggtgct actagaatgt ctgcaagaga attaaagtta
    13621 gagttagatc gttccaagaa taaaatgaag gaaaaaaata tcggtttgag gaaaaaaatt
    13681 cagcgaaatt ggttggagga aaggttagat gaagaaacat tgtcgaaact tgagaacatt
    13741 cgtagaaagc gttgaaatta ctaggttctt tagactataa tatacctata aatttgtttt
    13801 atatatgggg gagatttaca tgttggtagc aaaagaaaaa agttatgagt tagtggacaa
    13861 ttgtcagcag gatgagtata acattgtatt aagagcaagt gaaggggata aaatagcact
    13921 agagtacatt attactaaat acagaaattt tgtaaaggca aaagcaaagt catatttttt
    13981 aattggagca gataaagaag atataataca agaaggaatg ataggccttt ataaggcagt
    14041 tagagatttt gatggaagta agaccaattc atttaaatgt tttgcggaga tatgtataac
    14101 aagacaaata ataactgcta taaaaactgc aacaagacaa aaacatatac ctttaaattc
    14161 atatgtttct ttgaataaac caatatatga tgaagagtca gacagaacac ttttagatat
    14221 tatagctaca agtatagtaa cagatccaga agagcttata attagtaaag aagagttaaa
    14281 aaatatagaa tcaaaaatga acgaattgtt aagtgacttg gaattagaag tactagaatt
    14341 atatttaaat gggaagtcat accagtttat agcggacaag cttaaaagag atgtaaagtc
    14401 aatagataac gctttacaaa gggttaagag aaaattagaa aaacatcttg aaaacagaaa
    14461 tgattaatag taaaatactt accataagct tattgtgagc aaaattaatt tatatcgaaa
    14521 gataagggag gaaattcaaa atggctaaag ctaaatacga aagaacaaaa cctcatgtta
    14581 atatagggac aataggacac gtagaccacg gtaaaactac attaacagca gcaataacaa
    14641 aaacattata tgacagatat caattaggag aagcagtaga tttcgcaaac atagataaag
    14701 ctccagaaga aagagaaaga ggaatcacaa tatcaacagc acacgttgag tatgaaacac
    14761 caaatagaca ctatgcacac gttgactgcc caggacatgc tgactacgtt aagaacatga
    14821 taacaggagc agcacaaatg gacggagcaa tattagtttg ttcagcaaca gatggaccaa
    14881 tgccacaaac aagagagcat atactattat caagacaagt tggtgtacca tatatagtag
    14941 tattcttaaa caaatgtgac atggtagatg atgaagaatt attagagtta gtagagatgg
    15001 aagtaagaga tttattaaca gaatatgatt tcccaggaga tgacactcca atagtaagag
    15061 gttcagcatt aatggcatta gaagatccaa agagcgagtg gggagataag atagtagaat
    15121 tattcgagca aatagatgag tatataccag ctccagagag agatacagat aaaccattct
    15181 taatgccagt agaggacgta ttctcaatca caggaagagg aacagttgca acaggaagag
    15241 tggaaagagg agtactaaaa gtacaagacg aagtagaatt agtaggatta acagaagcac
    15301 caagaaaagt agtagtaaca ggagtagaga tgttcagaaa attattagac caagcacaag
    15361 caggggataa tataggagca ttattaagag gagtacaaag aaacgagata gaaagaggac
    15421 aagtactagc aaagactgga tcagtaaagg cacacacaaa gtttacagca gaagtatatg
    15481 tacttaaaaa agaagagggt ggaagacata caccattctt tgatggatat agaccacaat
    15541 tctatttcag aacaacagat gtaacaggag cttgtaagtt accagaagga atagagatgg
    15601 taatgcctgg agataacgta acaatggaag tagacttaat aaactcaata gttgtagaag
    15661 agggattaag attctcaata agagaaggtg gaagaacagt agcttcagga gttgttgcta
    15721 caataataga gtaattcttt actaaactaa tggatttaat ataaaaaaag agggggtaac
    15781 tcctctcttt tttgtttttt gttgacaagt agcgctaaag catatatact aaactagtgc
    15841 cttgagacag attaacaatc aagtttaagg ttatgatgtt gtaatagaaa ataaaattga
    15901 aaaaaacaga aaaaaaactt gaaaaaatgt aacagatgat ataaaatata atagtgtgct
    15961 tgagagatta agaaaataat aattaaatcc tagtgaaata cgaactgata tcgatttaaa
    16021 agttacaaca cacccggagc agtgtatcgg tattggttgt acgcaacttg aaaattaaac
    16081 acgtgataaa ggagggaaaa caaaatggct aagaatgaaa aaataagaat aagattaaaa
    16141 tcatatgatc acaaattatt agatttttca gcaggtaaaa tagttgaaac tgctaaaaag
    16201 gctggttcac aagtttcagg acctgtgcct ctaccaacag aaaaacaagt tgtaactata
    16261 ttaagagctg tacataagta caaagactct agagaacaat tcgaaataag aactcataaa
    16321 agactaatcg acatagctaa tccaacacct aagacagttg actcattaat gagattagac
    16381 ttaccagcag gtgtagatat agaaataaaa ttataagaac ccctaagata ttatcctata
    16441 gatgattatc ttagaatgat tactctaaaa gatgtaatcc gctgtaagaa ttataggagg
    16501 tgctaatatg aaaggaatat taggcaaaaa agttggtatg actcaaatat tcactgataa
    16561 aggtgtagtt atacctgtaa cagctgttga agcagggcct atggtcgtta ctcaaataaa
    16621 aacagttgat aaagatggat acaatgctat acaaataggt tttgaagatg ctaaagaaaa
    16681 agctttaaac aaacctaaaa aaggacattt agctgctgct aatgttttaa agaaacattt
    16741 aaaagaattt agagtagatt ctgtagaagg atacacagta ggacaagaaa taaaagctga
    16801 tgtatttgaa gcaggtgcaa aaatagatgt tactggaata agtaaaggta agggattcca
    16861 aggtccaata aaaagacatg gacaaagtag aggtcctgaa actcacggtt ctagatacca
    16921 cagaagacca ggttcaatgg gagcttgttc ttacccaggt agagtattta aaaacaaaaa
    16981 attagctgga cacatgggaa gcgtaaaggt aacagttcaa aacttagagg ttgttaaagt
    17041 agatgctgac aagaacctta tattagttaa aggagctata ccaggagcta aaggttcagt
    17101 agtaactata aaagaagcta taaaggtttc taaataatga ctaacctaga gaaaggagga
    17161 ataacaatgc caaaattaaa tgtactaaat gttagtggac aaaacgttgg agaaatagaa
    17221 ttatcagatt ctatatttgg cgtagaagtt aatggacatg ttttatacga agttgtaaaa
    17281 aatcaattag caaataagag acaaggaact caatctgcta aaactagagc agaagttaga
    17341 ggtggcggaa gaaaaccttg gaaacaaaaa ggaacaggta gagcaagaca aggttctaca
    17401 agatctgtac aatgggtagg tggaggagtt gcttttgcac ctaaaccaag aagctacaaa
    17461 tatacattac ctaagaaggt aagaagatta gctatgaaga gtgctttatc ttcaaaagtt
    17521 caaaatagtg aagttatagt attagatgca ttaaacatgg atgctcctaa gactaaagaa
    17581 tttgctcaaa tattaaacaa tataaatgct gctaaaaaag ctttagtagt tatagctgac
    17641 aaaaacgata acgtaataaa atcagctaga aatatagaag gtgttcaaac tgcattagtt
    17701 aatactatga atgtatatga tatattaaaa tacgattcat ttataataac tacagatgct
    17761 gttaaaaaag tggaggaggt gtacgcataa tgactaatcc acatgatgta ataataagac
    17821 cagttgtaac tgagcacagt atggctgaaa tgggtgaaaa aaaatacact tttgttgtgg
    17881 ctaaagatgc gaacaaaact gaaataaaaa aagcagtaga aaaagtattc ggagttagtg
    17941 ttgataaagt aaatacttta aactacgatg gaaaagttaa aagaatgggt agaactcaag
    18001 gaagaacagc aagctttaag aaagcagtag ttaaattaac tgctgatagt aaagaaatag
    18061 aattcttcca aggaatgtag tattataaca aacgaaggag ggaaaacaga tggctataaa
    18121 aaagtttaga ccaacttctc ctgccttaag acaaatgaca gttttagttt ctgatgaaat
    18181 aacatgtaat caaccagaaa aatctctttt agttaactta aagaaaaatg ctggtagaaa
    18241 cgtacatggt agaataactg ttcgtcatag aggtggagga caaaaaagaa aatatagaat
    18301 aatagatttt aaaagagata aagatggtat acctgctaag gttgctacta tagaatatga
    18361 tccaaacaga actgcaaata tcgcattatt aaactatgct gatggagaaa aaagatatat
    18421 attagctcca gttggaatta atgttggaga tactatatta tcaggattag gtgctgatat
    18481 aaaaccagga aactgtttag cattaaaaga tatgccagtt ggtactatca ttcataatat
    18541 agaattaaag ccaggaaaag gagctcaatt agttagatct gctggagttt ctgcacaatt
    18601 aatggctaaa gaaggaaaaa atgctttatt aagattacca tcaggtgaga tgagattagt
    18661 aagcataaac tgtaaagcta ctataggtca agttggaaat atagagcatg gtaacgtagt
    18721 tatcggtaaa gcaggtagaa aaagacatat gggaataaga cctactgtaa gaggttctgt
    18781 aatgaaccct aatgaccatc cacacggtgg aggggaaggt agatctccaa taggtagacc
    18841 atctcctgtt actccatggg gtaaaccagc tcttggatac aaaactagaa agaaaaataa
    18901 agcttcaaat aaactaatag tatcaagaag aactaagtag taaatttatt tttgctcgga
    18961 aaggaggaat ataaatgtca agatcaacta aaaaagggcc ttttgtccat gcaagacttt
    19021 taaagaagat agaagctatg aatgctagtg gaaataaaga agttataaaa acttggtcaa
    19081 gatcttcaac tgtatttcca caaatggtag aaaacactat agctgttcat gatggaagaa
    19141 aacatgttcc agtatatata acagaagata tggttggaca taaattaggt gagttcgttc
    19201 ctactagaac tttcaaagga cataaggatg acgaaaaatc taataagaga aaataattaa
    19261 attatgacta agaaaggagg aataacaatg gaagcaaaag ctactgctaa atacgtacgt
    19321 gtatcaccaa gaaaagcagg ccaaatatgt gaccttgtta gaggaaagaa tgttgatgaa
    19381 gcattagcaa tattaaagtt tactccaaga ggagcagctt caataatagc taaggttgta
    19441 aaatcagcta aagctaacgc agaaaacaat cacgaaatgg atactgaaaa attatatata
    19501 gcatcaatag ttgctaatca aggaccaaca atgaagagat tcatgcctag agctatgggt
    19561 cgtgcaacta caataagaaa aagaacttct catatagagg ttgttgttaa agagaaaaaa
    19621 taattaatag ataggaggga aagtaatggg tcaaaaggtt aatccacacg gcttaagggt
    19681 cggtgttata aaagattggg actcaagatg gttcgctact gataaaaaag agttcggaaa
    19741 cttattatta gaagaccata atatacgtaa attcttaaag aaaagattat actcagctgg
    19801 agttgctaag atagaaatag aaagatcagc taacaaaata aaaatggact tacacgttgc
    19861 taagcctggt gtagttatag gaagagctgg tgcaggaatc gaagcattaa aagctgaatt
    19921 agaaaaaatg acaaagaaaa ctataatagt taatatagta gaagtaagaa gtacagataa
    19981 aaatgctcaa ttagtagctg aaaatatagc tttagctata gagagaaggg ttgctttcag
    20041 aagagcaatg aaacaagcta tacaaagagc aatgaaatct ggtgctaaag ggataaaagt
    20101 ttctgcttca ggaagattag gtggagctga aatggctaga actgaaggtt atagtgaagg
    20161 aaatgtgcca ctacaaactt taagagcaga tatagattat ggtttcgctg aagctgatac
    20221 aacttatgga aaaatcggta taaaagtttg gatatgtaat ggtgaagttt taccaactag
    20281 agacggtgta aatccaagag aagaaagcag aaagagcgat agaagagaca acaagagaga
    20341 taatagaaga aacgatagaa gaggaaatga cagaagagga aatgataaca gaggaaacta
    20401 cagaggacaa agacctcaag gtggatcaag acctcaaaga actgaaaaca aaggaaatta
    20461 ataatagtta ggttcaagga aggaggaaaa atactcatgt taatgccaaa aagagtaaag
    20521 cgtcgtagag ttcatagagg aagcatggct gggcaagctc ataagggtaa taaagttact
    20581 tatggtgagt ttggattagt agcattagaa gcttcttgga taacttctaa tcaaatagaa
    20641 gctgccagaa tcgcgatgac tagatatata aaaagagggg gaaaggtttg gataaaaata
    20701 ttccctcata aaccagtaac aagaaaacca gcagagactc gtatgggtgc agggaaaggt
    20761 tctccagaat attgggtagc tgtagttaag ccaggaagag ttatgtttga attagcaggt
    20821 gtttctgaag ataaagcaag agaagctatg agacttgctg cacataaatt accaatcaaa
    20881 tgtaaatttg ttaaaaaaga agatttagaa gtaaagggtg gtgaatagga tgaaagctaa
    20941 agaattaaga gatttaacaa gcgaagagct aatgaacaaa ttaaacgact tcaaaagtga
    21001 attatttagc ttaagattcc aattagctac tggtcaatta gaaaatacag ctagaataaa
    21061 gtttgttaag aaggatatag caaaagttaa aactgttctt gctgaaagaa agttatacga
    21121 aactagagct taatttggaa aggaggcttt ttaaacatgg aaagaggaag aagaaaagtt
    21181 agaataggcc gtgttgttag tgataagatg gataaaacaa tagtagtagc tgttgaagaa
    21241 ttcgtacgtc atccactata taataaacgt gttaagagaa ctaagaaatt caaggctcat
    21301 gatgaaaaga atatatgtaa catcggagat agagttaaaa taatggaaac tagaccatta
    21361 tctaaagata agagattcag actagttgaa gttgttgaga aagttaagta gtttttgaag
    21421 aaggagggat tacgatatga tacaacaaga atcacgtcta agagttgctg ataactcagg
    21481 agctaaagaa cttttatgta tacgtgttct aggcggaagt aaaagaagat atggtaacat
    21541 aggcgacgtt atagttgcta ctgttaaaag tgcaacacct ggtggagttg taaaaaaagg
    21601 taaggttgtt aaagctgtta tagtaagaag caaacaaggc gtaagacgta atgacggaag
    21661 ctatatatct tttgatgaaa atgctgctgt tataataaag gacgataaaa ctccagtagg
    21721 aactcgtata ttcgggcctg ttgctagaga gttaagagac aatgaattca tgaaaatagt
    21781 ttctcttgct ccagaagtac tataattaag gaggtgcaat aggacatgat gcgtgttaaa
    21841 aaaggtgaca ctgttgtagt tatagcaggt aaagataaag gtaaaaaagg ttcagtttta
    21901 aaggtatatc ctaaaactag caaagtgtta gttgaaggtg taaatgtaat aactaaacat
    21961 caaaaaccaa gtgctatgaa ccaacaaggt ggaataataa ataaagaagc cccaatacac
    22021 atatctaatg taatgccatt tgatcctgaa acaggaaaag gcgttagagt aagatatgaa
    22081 gtaaaagatg ggaacaaagt aagagtatca gcaaagagcg gaaaagaatt ataataaata
    22141 ttgcttgaaa ggagggacct taaatggctt ctagattaca agaaaaatac atgaaagaag
    22201 ttgctcctgc tttaatggag aaatttggat acaaaaacgt aatggagata cctaagttaa
    22261 ataaaatagt tattaacatg ggtataggtg acgctagaga aaatccaaaa ggattagaaa
    22321 aaggcgttga agaattagaa ataatatcag gacaaaagcc tgttataact aaagctagaa
    22381 aatctgttgc taacttcaaa ctaagagaag gaatgccaat aggaacaaaa gttacattaa
    22441 gagctgataa aatgttctac tttatggaca aattagtttc agtttcttta ccaagagtta
    22501 gagactttag aggagttaac cctaatgctt ttgatggtag aggaaattat gctttaggag
    22561 ttaaagaaca attaatattc cctgaaatag aatatgataa aatagataaa gtaagaggaa
    22621 tggatataat atttgttact acagcaaaga ctgacgaaga agctcgtgaa ttattaaaat
    22681 tattaggaat gccattttct aagtaagcaa aggagggatt cccgtggcta gaaaagcgat
    22741 ggttgttaaa caacaaagaa agcaaaagta cgctactaga gaatacacta gatgtactat
    22801 atgcggaaga ccgcattcag ttttgaaaaa atttggtata tgccgtatat gctttagaga
    22861 attagcttat aaaggtcaaa tacctggtgt tagaaaagca agctggtaga agcttgatta
    22921 gaatggaagg agggcaacaa aatgacaatg acagatccaa ttgcagatat gcttactcgt
    22981 ataagaaatg ctaatgttgt taagcatgaa actgtagatg ttccagcttc taatatgaag
    23041 aaagaattag ctagaatctt attagaagaa ggtttcataa gaggttatga tgttatagaa
    23101 gatggaaaac aaggtatcat aagaatacaa ttaaagtacg gacaagaagg cgaaagagtt
    23161 ataacaggtc ttaagaaaat atctaagcct ggaatgagag tttacgctgc taaccatgag
    23221 ataccaaaag tattaaatgg attaggaata tcagttatat ctacttcaaa aggaatttta
    23281 actgacaagc aagctagaaa agaaaatgtt ggtggagaag taatctgcta cgtttggtaa
    23341 ttaatatgaa caatgtaagg aggtgcgacc atgtcaagaa taggtgttaa gccaataata
    23401 ataccagcag gtgttgaagt tactatagct gaaggaaatt tagttacagt aaaaggtcca
    23461 aaaggaactt taactaaaca attaagtgct gagttaaaca taaaaaaaga agaaaataca
    23521 ataatggttg aaagaccaac taataataaa aaacatagat ctttacacgg actaactaga
    23581 actttattag ataatatggt tgtaggtgta aatactggtt tcgaaaagaa attagaatta
    23641 aaaggtgttg gatatagagc tcaaaaacaa ggaaagaaat tagttatgaa cttaggtttc
    23701 tctcatccag ttgagatgga agatccagaa ggaataactg ttgaagctcc aaaccaaaca
    23761 gagttaatag taaaaggtat agacaagcaa ttagtaggga attatgctgc taagataaga
    23821 gcttggagaa agccagagcc atacaaaggt aaaggtataa aatacgttga tgaagtaatc
    23881 agacgtaaag aaggaaaaac tggtaaaaaa taataccaaa acttaaacgc tagaaaggag
    23941 tgagctaggt gttaaaaaag gctgataaaa atgctaatag acttcaaaga cataagagag
    24001 ttcgtagaaa aataagcggt acttctcaaa gaccaagatt atgtgtattt agaagtgcta
    24061 acaatatata tgctcaaata atagatgaca caaaaagagt aactctagtt gctgcatctt
    24121 ctttagaagc agaagttaag agtgctgtca atcatacagg aaacaaggaa gcagctaaga
    24181 aagttggaga attagttgct aaaaaagctg ttgaaaaagg aatcactgaa gttgtatttg
    24241 acagaggtgg atacttatat catggaagaa ttcaagaatt agctgaaggt gctagagaag
    24301 ctggtcttaa gttctaatac gaaggaggga aaaatacatg ctacgtcgta agccgataga
    24361 tgcaggacaa cttgacttac aagaaaaagt tgttgaagta agacgtgtta ctaaggttgt
    24421 aaaaggtggt agaaatttca gatttgcagc attagtagtt gttggagatg aaaacggaca
    24481 cgttggtata ggtgctggga aagcaatgga agtaccagac gctataaaaa aagcagttga
    24541 agatgctaag aaaaatctaa tagtagtgcc aatagttggg actacaattc ctcacgaagt
    24601 acgcggacac tttggtgctg gaaatatatt aataatgcct gctgtagaag gtacaggagt
    24661 aatagctggg ggacctgcta gagccgtact tgagctagct ggactaaaag acgttagagc
    24721 taaatcttta gggtctaaca atccaagaaa tatggtaaat gctacaatag aaggacttaa
    24781 ttctctaaaa acagttgaag atatagctaa acttagaggt aaaaaagttg aagaacttct
    24841 agggtaagga ggtagtgtaa aatggctaaa ttacaaataa agttagttag aagtgttata
    24901 ggaactactc ctaaccaaaa aaagaatgtt gaagcgttag gattaagaaa aagagaacaa
    24961 gttgttgtaa aagaagacaa tgctcaaaca agaggtatga taaataaagt aagtcactta
    25021 ttagaagtaa ctgaaatagc tgaataattt ttataaagta gtgcaaggag gtgcaatcca
    25081 tgaagttaca tgagttaaaa cctgctgaag gtgcagtaag agctaagaga agattaggta
    25141 gaggtactgc aactggacaa ggtaaaactg caggacgtgg acaaaaaggt caatggtctc
    25201 gttctggtgg tggagtaaga gtaggatttg aaggtggaca aatgccacta gctagaagac
    25261 ttcctaagag aggtttcaat aacatattta agaaagttta cacagaagtt aatgttgaag
    25321 ttttaaacag atttgaaaat ggaacagaaa taactgcaga attattaaaa tctactaaaa
    25381 ctataagcaa aataggtaaa gatggaatta aaatcttagg tgaagggaat ttagagaaag
    25441 ctttaactgt taaagctgct aaatttacag cttcagctca agaaaaaata gaaaaagctg
    25501 gtggaaaagc agagttagta taataacctg ctaatctcct agccagtaca ggcggggtga
    25561 attagcgtgc tgtcaaaatt aaaacaagct tggaagatta aagcagtaag aaaaaaagta
    25621 atgtatacac ttatgatgat cgtgatattc agaataggta ctactatacc agttccaggc
    25681 atagacacta gtattataca aaagatggta ggcggcaata gtctactttc tctgtataat
    25741 atgtttactg gtggagcatt tagtaacttt tcactatttg ctttaggtat aagtccatat
    25801 atcactgcat caatcataat acaacttcta acagtaggtt ttgaaagttt agcagaactt
    25861 caaaaatctg gtgaagaagg aaagaaaaaa atcaataagt acactaaata tacagcacta
    25921 gctctagcag ttgtacaagc tttgggaatt acattaggaa ttgtaagaag tgctttaata
    25981 tcaaatagtg tgttttttat cacaactgtt gtaataacat tagtttcagc aagtatgtta
    26041 gtaatgtgga ttggggacaa aattactgaa aaaggaatcg gtaatggtag ttcagtaata
    26101 atatttgcag gtataatatc aaggatacct acagatgtta ttaaaatatc gcaacaagtt
    26161 aaatctggcg aagttgcacc ttgggttata gtgattctag ctgtagttat attacttaca
    26221 gtaacaggag ttacatttat acaagaagca actagaaaga tacctgtaca atatgctaaa
    26281 agagttgttg gaagaaaaat gtatggagga caaagttctc atataccaat gaaagttaac
    26341 cagtcaggag ttatgcctat aatttttgct agttcacttt tagctttccc tcaaacaata
    26401 gctatgttta tgggtccaaa tgctcaggct tttgttcaaa aatacttaag tatggcaaca
    26461 gaacaaggat tttggacata tagatcaatt gagattctac ttataatttt cttctcatat
    26521 ttttatacaa ctgtatcatt taatacagaa gatatatcta aaaatatgaa aaataatggt
    26581 ggatttatac caggtataag accaggtgaa cctactatgg attatttaaa tagaatttta
    26641 actagattaa ctttagctgg agctactttc ttagcaataa ttgctatggt gccagctctt
    26701 acaacacatt atatgaaagt aaatatgagt ctagctggga cttcattgct aatcgttgtt
    26761 ggagttgctt tggagcttaa gagacagtta gagtcaaact tagttatgag aagctatcaa
    26821 ggtttcttaa aataaaatgg agatgggata atatgagaat aatattactt ggacctccag
    26881 gtgctggtaa aggtactcag gcggcaggaa tagtagaaaa atacaatata cctcatatat
    26941 caacaggaga tatattcaga aagaatataa aagagggaac agaacttgga aaaaaagcta
    27001 aagaatacat ggaccaaggt ttattagtac cagatgagtt aactgtaggt ttagttactg
    27061 atagaatatc tcaagaagat tgtaaaaatg gatttatgtt agatggattt ccaagaaatg
    27121 tagcacaagg agaacattta gatatcttct taaaagatgc tggtatatca ctagataaag
    27181 ttgtcaatat tgaagttgat aagagtatat tagtgtctag agcagttggt agaagaatat
    27241 gtaagtcttg tggagctact taccatgttg agtttaatcc tcctaaagta gaaggtgtat
    27301 gtgatgtatg ccaaggagaa ttatatcaaa gagctgatga taatgaagaa actgtatcta
    27361 agagaataca agtttatcta gatgaaacta agcctttagt agattattat agcaaacaag
    27421 gtataatagc agatataaaa ggtgatcaag caatagataa agtatttgaa gatattgtcg
    27481 cagccttagg aagtggaaaa taaatgatta tcttgaaatc aaaaaaagaa atagaacttt
    27541 taagagaagc aggcaaaata gttgctgaca ctcatgaagt tttaagaaaa gctatttctc
    27601 cgggaatatc tactttagaa ttagataaaa tagctgaaga gaatataaga aaatacaatg
    27661 cagaaccttc ttttaaagga tatggaggtt ttccaggaag tatatgtgct tctatcaata
    27721 gagaggtagt acatggtatt ccaggagaaa ctatattgca agaaggagac attgtaagtt
    27781 tggatatagg agcctattat aaaggatatc atggtgattc tgcgaaaact cacggagtag
    27841 gcatgatatc tgaagaagat aggaaattga tagaagtaac tagagaaagc ttctatgaag
    27901 gaataaaatt tgctaaatta ggatatagac tttcagatat ctcacacgca gtacaaacac
    27961 acgttgagaa acatggtttc tcggtagtta gagatttagt gggtcatgga gttggagcaa
    28021 atcttcatga agacccacaa gtacctaatt atggactccc aggaaaagga ccaagactta
    28081 gagaaggaat ggttattgct atagagccaa tggttaatgc aggtcgttat catgtaaaga
    28141 ctctatcaga tgggtggacg actgttacaa tagatggaaa aaaatcagct cattatgagc
    28201 atacgatagc aattactgaa catgaacctt tgatattgac aaagttgtaa tttaaaaagt
    28261 aggtgaaaaa agtgctatca gataatttaa gtataggtca agttgttaaa gtttcattag
    28321 gacgagataa aggaaattta ttctttgtag tcaaaataat taataatgaa tatgtattaa
    28381 tagctgatgg aaaaaagaga aaacttgaca aacctaagtt aaagaaagtg aggcacttaa
    28441 aaaaatataa ttttattaat gatgaagtta gaaaaagagt agtatctgga caagagatta
    28501 cagattcttt tttaagagct gaacttacta agttaaatta gataatttaa cttattgaaa
    28561 tggagggctg gttagttaat ggccaaaaaa gatgttatag aattagaagg tacagtttca
    28621 gaagctttac ctaatgctat gttcaaagtt aaactagaaa atggacatga gatattatgc
    28681 cacatttctg gaaagctaag aatgaacttc ataagaattc ttgaaggtga caaggtgaat
    28741 gttgaacttt ctccatatga ccttacaaga ggaagaatta cttggcgtaa gaagtagact
    28801 atattaatat ttagtctaag gagggattaa taatgaaggt tagaccatca gtaaaaccaa
    28861 tatgtgaaaa atgtaaagta ataaaaagaa aaggaaaagt aatggttatc tgtgaaaatc
    28921 caaagcataa gcaaaagcaa ggataagaaa ttattactag cattttgtta aattttatag
    28981 tataatacta tattgtgatg ttaacaaaag aacgttaaaa tcgttgaaca atctggcgta
    29041 ggattggcat atgatgtgaa gttttaaaca ctaaaaacta tgtgtaggag gtgcgatcat
    29101 ggcaagaata gctggggtag atttacctag agaaaaaaga gcggagatag gcttaactta
    29161 tatatatggt ataggtaaag cgactgctaa cgaaatatta gctaaggctg agataaatcc
    29221 agatactaga ataaaagatt tatcagaaga tcaagttaat gagttaagaa aagttataga
    29281 tgacgatttc ttagttgaag gtgacttaag aagagaaatt gctttaaaca taaagagatt
    29341 aagagatata aaatgttata gaggaataag acacgctaaa ggtcttccat taagaggaca
    29401 aagaactaag actaatgcta gaactagaaa aggtcctaga aaaacagtat ctcgtaaaaa
    29461 gaaaaaataa ataaataata actagatgaa ggagggaaaa ggaaaatggc taaaccaaaa
    29521 aagaaagtta cacgtattag aagaagagaa cgtaaaaata tagaacgtgg tcatgctcat
    29581 atacaatcaa ctttcaataa tacaataata actttaactg acgttcacgg aaatgctata
    29641 tcttgggcaa gttctggaca attaggattc aaaggatcaa gaaaatcaac tccatttgca
    29701 tctcaaatgg ctgctgaaac agctgcaaaa gctgcgatgg aacacggact aaaaagtgtt
    29761 gaggtattcg taaaggggcc aggttcagga agagaagctg caataagagc tttacaagca
    29821 actggactag aagtaactat gataaaagac gttactccaa tcccacataa cggatgcaga
    29881 ccaccaaaga gaagaagagt gtaattaata agagtacaat aggaggtgta gataaatggc
    29941 aagatataca ggtgcatcat gtagacaatg ccgtagagag ggaatgaaat tattccttaa
    30001 aggtgataga tgttatacag ataaatgtgc tatagtaaaa agaaactatg ctccaggtca
    30061 acatggccaa ggaagaaaga aagtttctaa ctatggatta caattaagag aaaaacaaaa
    30121 agttaaaaga atatatggag ttttagaaac tcaatttaga aatttatatg aacgtgctga
    30181 aaatatgcct ggtaaagcag gtgaaaactt attaagttta ttagagagaa gattagataa
    30241 tgtagtttac agaatgggac tagcatcttc tagaaaagaa gctagacaat tagtgactca
    30301 tggtcatttc actttaaatg gaaataaagt agacatacct tcattaatag ttaaggttgg
    30361 agatgttata gaagttaaag agaaatcaag atcttctgca aaattcaaaa atttagtaga
    30421 agttaactca agaatagctc ctaagtggtt agaagctaat gtagaaggaa tgacagcaaa
    30481 agtagttggt gttccaacaa gagaagatat agatcttgag atagcagagc acttaatcat
    30541 agaactttac tctaaataat aagcacactt ttaaaaatta ttttaaaatt ttgtttaccc
    30601 tcagtggatt actgaatttt aaggagggtt ttgtccatga tagaaataga aaagccaaaa
    30661 gtagatatag ttgagcttag cgaagactat agatatggta agtttgttat agagcctcta
    30721 gaaagaggat atggaataac tataggtaac gcattaagaa gaatattatt atcatcacta
    30781 ccaggtgtag cagtgaatgc tataaaaata gatggagttc ttcatgaatt ttcaacaata
    30841 cctggtgtta aagaagatgt tactgagata atattaactt taaaagagct ttcagcaact
    30901 atagatggtg aaggaagtag aacacttaaa atagaagctc aaggaccatg ctctatcaca
    30961 ggtgcagata taatctgtcc tccagatgtt gaaatattaa gtaaagattt agcaatagct
    31021 acattagatg ataatgctaa acttaatatg gagatatttg tagataaagg tagaggttat
    31081 gtttctgctg aagaaaataa aacagagaat gttccaatag gtgttttacc tgtggattca
    31141 atatatactc ctgttgagaa ggttagttat catgtggaaa acacaagagt tggtcaaaaa
    31201 acagattatg ataaattagt actagaagtt tggactaatg gtagtataaa tcctcaagag
    31261 ggcatatcat tagctgcaaa ggtattagtt gagcatttaa acttatttat agacttaact
    31321 gaacatgtaa gtagtgttga aatcatggta gaaaaagaag aagatcaaaa agaaaaagtt
    31381 cttgaaatga ctatagaaga attagattta tcagttagat catataactg tttaaagaga
    31441 gcgggaatta atacagttga agaattagct aataaatctg aggatgatat gatgaaggtt
    31501 agaaacctag gtaagaagtc attagaagag gtaatacaaa aactagaaga acttggattg
    31561 ggattaaaac caagtgaaga atagcgagca aaggagggat aagaatggct aagtaccgta
    31621 aattaggacg tgaaacagct cacagaaacc ttatgttaag aaacttagta acttgcttac
    31681 taagaagtgg aagaatagaa actacagtga ctagagcgaa agaaactcgt agaatggctg
    31741 aaaagatgat aactcttgct aagagagggg atcttcatgc tagaagacaa gttttagctt
    31801 atgttatgga tgaaacagta gttaataact tattcacaga tttagctcca aaatatgctg
    31861 agagaaatgg tggatacact agaataataa aaatagggcc aagaaaaggc gatgctgctg
    31921 aaatggcttt tatagaatta gtatagtaag gaaaggatta ggcttgatgg cttaatcttt
    31981 tttttatttt tttgactaag gtgtgatata ataatgtgta aaatgatttt tataagttat
    32041 tgcttactaa aaggagtatg agttaatgga taatatagta aaggtaaata atatttcgtt
    32101 tgagtatatt acagatgaag caaaacttaa agcaatagat gatttaagcc tagatgttaa
    32161 aaaaggagaa tttgttgcga taataggaca taatggttct ggtaagtcta ctttatctaa
    32221 aaacttaaat gctattctta tgcccactga aggaaatatt cttattgatg gtatggacac
    32281 taaagaagaa gaaagattat gggatataag acaaactgca ggcatggtat ttcaaaatcc
    32341 agataatcaa atagttgcta ctatagtaga agaagacgtt gcatttggac ctgagaactt
    32401 aggtatagaa ccaaaagaaa taagacgaat tgtagaagag tctttaaaaa gtgtaggtat
    32461 gtatgattta agagatagac agcctcactt gttatcagga ggtcaaaagc aaagagttgc
    32521 aatagcagga attattgcta tgaggcctaa atgtataata tttgatgaag caacagcaat
    32581 gttagatcca tctgggagaa aagaagttat gaaaacaata aaaagactta ataaggaaga
    32641 aaatataact gttatacata taacacactt tatggaagaa gcagtagaag ctgatagagt
    32701 tgtagttatg gaaaaaggaa agaaaatctt agagggaact cctagagaag tattcagcaa
    32761 aataaagatg ttaaaggaaa tcggtttaga tgtaccttgt atgacagagt tatctagttt
    32821 attaatagaa gaaggtataa atattagtag tgatatatta actgtggatg agatggtgat
    32881 ggaattatgt caattatagt aaagaattta acacatatat ataatgaagg gatgcctttt
    32941 gcaagtaagg cacttgatga tgtttctttt gagattaaag atagagattt tgttggtctt
    33001 ataggtcata caggctcagg aaaatctaca ttaatacaac atttaaatgg attactaaag
    33061 ccttcttcag gagaaatatt tataaatgat tttaatataa cagataaaaa cttgaattta
    33121 actgagatta gaaaaagagt aggggttgta tttcaatatc cagaatacca attgtttgaa
    33181 gaaacgattg ataaagatat agcttttgga ccttctaatt taggcttaga ggagtctgag
    33241 atacataaca gagtaaaagc atcgatggaa gctgtagggc ttgagtatga ggaatttaaa
    33301 gacaaatctc cttttgaatt atctggagga caaaagcgta gagtagcgat tgccggagtt
    33361 atagccatga acccagaagt tctcatattg gacgaaccaa ctgctggtct tgaccctggt
    33421 ggaagagatg aaatttttaa cttaataaaa gacttacatg aaaaaaagaa tatgactata
    33481 atactatcat cacatagtat ggatgatatg gcaaagttag caaaaacttt aattgttatg
    33541 aatcatggta gcgtagaatt tatgggaact cctagagaag tatttaaatc aaatgctagt
    33601 aaattaaaag atataggatt agatattcca caagttttag agcttgcatt gaaattgaga
    33661 gaaaaaggct ttgacatcag tgaggatata ttgactttag aagaagcaaa acaggaaata
    33721 ttaaaagttg tgagaggacg aggattatgt taaaagatat aactataggg caatactacc
    33781 caacaagttc tgctattcat aaattagatc caaggataaa gcttgtagcg acaattgtat
    33841 ttatggtatc tatatttgta gtaaataaat tttggcctta tatagtagtt ttattatgtt
    33901 tattagctat gataaaattg gctaatatac cagtcaaata tattgttaag ggtgttaaac
    33961 ctcttaaatg gattattttg tttacatttc ttataaatat ttttttctta ccaggtgatg
    34021 aaatatggtc atttggattt ttagctatta caaaacaggg tctaagacaa gcaattttta
    34081 tggcaataag attaatattt ttagtagttg gtacatcatt gcttacttta acaacttcac
    34141 caatagaatt aactgatgga atagagagat tgttgaatcc atttaagaaa attggtttgc
    34201 cagttcatga acttgcaatg atgatgacaa ttgctttacg ttttattcct accttgttag
    34261 atgagacaga caaaataatg aaggctcaga tgtctagagg tgcagatttt gaaagtaaga
    34321 atcttataaa tagagcaaag aatttagtac cacttttggt gcctttattt gtgagtgcat
    34381 ttagaagggc tgatgaattg gctatggcca tggaggctag atgttacaga ggtggacata
    34441 atagaactaa gatgagagag tctgttatat caagaaggga ttatatggca tgtgtatttc
    34501 aggttgttta tctgggagca attatagcta ctagatttat agcaatttaa ttaataagct
    34561 atagatataa tgaggtaaag tatgagaaat ataaaaatta caattcaata caagggtaag
    34621 aattattgtg gatggcaaaa gcaacccgat tctttaggga tacaaggtac aattgaaagg
    34681 gctatatatg atataacaaa agaagaaacc aatcttatag ggtctggtag aactgattca
    34741 ggagttcatg ctattggtca gattgcaaat tttaaaatta attcaggtat aagtatagaa
    34801 agtataccaa tggcactaaa tgccaagttg ccaaaagaca tatcagttat agaagcatgt
    34861 gaagtaaatg atgattttca ctccagatac agtgcaaaag gtaaaacata taaatattta
    34921 gtatataata gcaagtttag aaatccaata cttagtgaga tatcatatca agtaaaatat
    34981 gaacttgatt ttgataaaat gtgttcggaa gcaaaaagtt tattaggaac acatgatttt
    35041 aaaggtttta tgagttctgg ttcatctgta aaagatacag ttagaacgat atatgatatt
    35101 gatataagta aaaaagatga cttgattact tttgagatta gtggtaatgg ttttttatat
    35161 aacatggtga ggattattgt tggaacactt gttgatatgg gtcgtggtag aattaacgaa
    35221 ccatttttag atataataca atcaaaaact aggtcaagat gtggacatac agctccagca
    35281 caaggattat ttctcaaaaa agttcattat taacttgaca caccaggcta catgtattaa
    35341 aatatatgag aatgtgttaa taagtccact tgccccggac ttgacataaa cggtaaaaaa
    35401 attttatgaa aagtagttaa ggagggacag ttatgaaaag ttatatagct aagccagctg
    35461 atgtacaaag aaaatggtac ttagttgatg ctgaaggaaa aacattaggt cgtttagcaa
    35521 cagaaatcgc gacagtattg agaggtaaac ataaaccaac atttactcct cacgttgatg
    35581 gtggagattt tgttgttgta gtaaatgcag aaaaaatagt tttaagtggt aagaaattag
    35641 atcaaaaata ctacagatac catactggtt atgtaggtgg attaaaagaa atatcttaca
    35701 gagatatgat ggataagaaa ccagaagaag ttatatcaca tgctgtaagc ggtatgttac
    35761 ctaaaaataa attaagaagc agaatgatga ctagattaag agtatttgca ggtgctgagc
    35821 acactcatgc agctcaaaat ccagaagttt taaactttaa ataagattaa taggtgaaag
    35881 gaggagttta taatggctaa cgttcaatat tatggaactg gaagaagaaa aagttctgtt
    35941 gctagagtaa gactagttgc aggtgaagga aatatattag taaatggaag agcattagaa
    36001 aattatttta attatgagac attaataaga gatgttaaac aaccattagt tttaactggt
    36061 aatgaaaata aatatgatgt tatagtaaaa gttgaaggtg gaggattcac tggacaagct
    36121 ggagctataa gacatggtat atctagagct ttattaaaag ctgatttaga tttaagacct
    36181 gctttaaaga aagaaggttt cttaactaga gatgcaagaa tgaaggaaag aaagaaatac
    36241 ggattaaaag cagcaagaag agctccacaa ttctcaaaaa gataattact tatttttttg
    36301 gaaaagcaaa atcctttatt tactgttatt cagaaagtat tggtatttta aaaatgttgg
    36361 gttaagttaa ttattgatat ataataaaat aataagatat cttattagga tgtttttcat
    36421 taagaagagc atccttttta atttgactat ttaataaaaa acatatgc
//
LOCUS       DAEMAE010000013        34886 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEMAE010000013 DAEMAE010000000
VERSION     DAEMAE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.013"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..394)
                     /locus_tag="KPH93_000575"
     CDS             complement(<1..394)
                     /locus_tag="KPH93_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG1687412.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYVKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYK"
     gene            complement(586..1773)
                     /locus_tag="KPH93_000576"
     CDS             complement(586..1773)
                     /locus_tag="KPH93_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG1687413.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFRDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPRLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFEFNEHTAKLMKQSFIEIIRMIFGIRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIESLGNIPNNF
                     KVKNSVEQITILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVANRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1805..2230)
                     /locus_tag="KPH93_000577"
     CDS             complement(1805..2230)
                     /locus_tag="KPH93_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687414.1"
                     /translation="MINENLRSLRKIHQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYKVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVPKEE"
     gene            complement(2668..4617)
                     /locus_tag="KPH93_000578"
     CDS             complement(2668..4617)
                     /locus_tag="KPH93_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1687415.1"
                     /translation="MERKYQNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGIEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVPVLKSKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYEMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVDNENVDALIIAVGSEPLVPPIEGLNGENVVIVNDYYLEKE
                     KVTDEVVVLGGGLAGCEAAIHLAQEGKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDCAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            complement(4589..5887)
                     /locus_tag="KPH93_000579"
     CDS             complement(4589..5887)
                     /locus_tag="KPH93_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBG1687416.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEYLNSFAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVTNW
                     IINKYGVTSAFKSIGLIFLIIVCVSSFFIKKCPADFTPEGWVAPEKKGKVRVIINKNW
                     KEMLLTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDAAIVVSILALFNTF
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYRYINKIQNLNIENLMKGDIKDGKKISKSM"
     gene            6139..6696
                     /locus_tag="KPH93_000580"
     CDS             6139..6696
                     /locus_tag="KPH93_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1687417.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITKKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            7140..7889
                     /locus_tag="KPH93_000581"
     CDS             7140..7889
                     /locus_tag="KPH93_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG1687418.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISV
                     DKPFIFVCPTYAWRLPIVVTDFIKKTKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(8050..8415)
                     /locus_tag="KPH93_000582"
     CDS             complement(8050..8415)
                     /locus_tag="KPH93_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1687419.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITEKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(8431..9207)
                     /locus_tag="KPH93_000583"
     CDS             complement(8431..9207)
                     /locus_tag="KPH93_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1687420.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKRQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSSK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(9600..11027)
                     /gene="ascB"
                     /locus_tag="KPH93_000584"
     CDS             complement(9600..11027)
                     /gene="ascB"
                     /locus_tag="KPH93_000584"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG1687421.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPTGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     KVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(11088..13016)
                     /locus_tag="KPH93_000585"
     CDS             complement(11088..13016)
                     /locus_tag="KPH93_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687422.1"
                     /translation="MGKYKQLAQEIVKNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGTDVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNRFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGLFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKIRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVGAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(13161..14003)
                     /locus_tag="KPH93_000586"
     CDS             complement(13161..14003)
                     /locus_tag="KPH93_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1687423.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHKGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELICLTIHLAKVVK
                     DSNM"
     gene            complement(14320..15720)
                     /locus_tag="KPH93_000587"
     CDS             complement(14320..15720)
                     /locus_tag="KPH93_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1687424.1"
                     /translation="MKNKYEDVAQLIIDGLGGKNNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINEGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNISIAVIVVGVFLHPNFSAWISSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPVMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVDRV"
     gene            complement(15759..16430)
                     /gene="pgmB"
                     /locus_tag="KPH93_000588"
     CDS             complement(15759..16430)
                     /gene="pgmB"
                     /locus_tag="KPH93_000588"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG1687425.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWYEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINGLIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSCSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            complement(16432..18828)
                     /locus_tag="KPH93_000589"
     CDS             complement(16432..18828)
                     /locus_tag="KPH93_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG1687426.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYIDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGKKVKVYIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSISMEKFNCEKVDDPRVASINKKQFKIDIKKYNNKYFMNLVTE
                     RSKLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYS
                     DLNLNEERLKSLECLESLKCLESEAHLKSLELVLREACDIGFDKVKNIQREKMNKFWT
                     NSDVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYIL
                     PFFIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGT
                     AQFHINADIAYGIRTYFEATNDEEFMSQNGLEILIETARFWVEFGDYIESKNNKFCIN
                     GVTGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKN
                     WINAAQNMYLPYDEEKKISKQDDSFLEKSVWNFKNTPKEKYPLLLNYHPLTIYRYQVN
                     KQADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYN
                     YFMDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWK
                     NIKFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNVIEVNNCFKRSN
                     "
     gene            complement(18854..20302)
                     /locus_tag="KPH93_000590"
     CDS             complement(18854..20302)
                     /locus_tag="KPH93_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG1687427.1"
                     /translation="MKLRKDFLWGGAISANQSEGFYLSGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWANRKVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRCINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNKIKLIIKEEDLSTLKNNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSIWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVTEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELLGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(20707..21594)
                     /locus_tag="KPH93_000591"
     CDS             complement(20707..21594)
                     /locus_tag="KPH93_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1687428.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGKVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKISKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGVSK"
     gene            complement(21693..22142)
                     /locus_tag="KPH93_000592"
     CDS             complement(21693..22142)
                     /locus_tag="KPH93_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1687429.1"
                     /translation="MEIREILTEESIILDLKSTIKEDIIQELVDKLYDTGSITLKSKF
                     IKALKRREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSEKGVDWPSIDGLPVKVIIM
                     FAIPQSAGNEDMILISKIAYILGGKNISERLLNAHTKKEILDIFYEV"
     gene            complement(22132..24039)
                     /locus_tag="KPH93_000593"
     CDS             complement(22132..24039)
                     /locus_tag="KPH93_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1687430.1"
                     /translation="MLVSRQIEFLKLILSENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSVTFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREVSVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSESNSSYNDESDRLSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFIISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVVQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVNRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKNKEDIKILL
                     DNNLLEKTNGN"
     gene            complement(24322..26217)
                     /locus_tag="KPH93_000594"
     CDS             complement(24322..26217)
                     /locus_tag="KPH93_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1687431.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVIDYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVLLAKDLIINAENGMAKEIKPGGKTLEDSK
                     EILQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(26253..27131)
                     /locus_tag="KPH93_000595"
     CDS             complement(26253..27131)
                     /locus_tag="KPH93_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG1687432.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKVAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(27836..29269)
                     /locus_tag="KPH93_000596"
     CDS             complement(27836..29269)
                     /locus_tag="KPH93_000596"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG1687433.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGIIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPNGDESIPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     SRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     NTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNNSAGKA
                     DVVENPYLKTSDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDILKSDNTVDD
                     DYRIEYLANHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFYWYKKVIKFNGEIL"
     gene            complement(29292..31133)
                     /locus_tag="KPH93_000597"
     CDS             complement(29292..31133)
                     /locus_tag="KPH93_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687434.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLGANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDAISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDKIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(31259..32092)
                     /locus_tag="KPH93_000598"
     CDS             complement(31259..32092)
                     /locus_tag="KPH93_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1687435.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNSEESLSELHSLLDRIPLKDVELVSEIIQMGREELSDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKDIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(33137..33349)
                     /locus_tag="KPH93_000599"
     CDS             complement(33137..33349)
                     /locus_tag="KPH93_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687436.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIVKVFDTTVENLFEFIEEE"
     gene            complement(33350..33766)
                     /locus_tag="KPH93_000600"
     CDS             complement(33350..33766)
                     /locus_tag="KPH93_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687437.1"
                     /translation="MSKSETGVKSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNIDTGIILIAVLFIQSLSKLVLYMYFDKRGICN"
     gene            complement(33834..34247)
                     /locus_tag="KPH93_000601"
     CDS             complement(33834..34247)
                     /locus_tag="KPH93_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687438.1"
                     /translation="MSKSELGVKTNLVSELLMMSYFFIDSLLEKFIFKGGNIWITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSIFLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIYFDKKGICN"
BASE COUNT        11401 a         6191 c         3655 g        13639 t
ORIGIN      
        1 gcttatattt aagatatatt ggtggatact ttacactttt taattcctca ttacttctta
       61 aaagtttatt attattttcc atatcataaa tttgtaacat tactttattt acatatagag
      121 cataataatt atcactataa aaatattgat actcatatcg agggtcatct ttataataca
      181 ctttgtatag tattccttcg taacttgcct tcttggcttt ttcttttgta atacttttta
      241 tattatcttt tgaaatatct tgctttacca tatacttatc aatatatgtt tcagcatctt
      301 tccatggttt aacatagttt ttatatacaa caaaccctgt tacacttata atgcctataa
      361 ttaatactat aaaaataact ttatatttct tcatattaat cctccttcat ttattattta
      421 aaacttgata taatgcatta aaaaaaggat gttatctttt ttattaaaat atagcatccc
      481 tcatatttag catcaatata tatgccccaa ctggtaggtt aaaagacccc actgtcagta
      541 ctttgattaa attacatgta tgtatatatt tttttaacaa atctattata tgtcaacttg
      601 tctactatta catataatct gtaatattac atctgctgct ttttttgagc ctcctgcatt
      661 ataaaagctt tttgatagtt tagttgcatt ttctttatat tcattgtcat ttataacttg
      721 aaatactgct tttttaatac tttctgactt atcctctttt aacataattc ctgctcccaa
      781 atgaacaact ctattagcta ccattctctg ctctgaatgt tgaggaaata ataccatggg
      841 tactccataa tataaacttt cattaacact attcatccca gaatgagtaa caaacacatc
      901 agttttttga agtatagtaa tctgttcaac actattttta accttaaaat tatttggtat
      961 atttcccaaa ctttcaatat cagtacttct accaaccgac ataatcacat caacatcaga
     1021 atctttaaat gcatttatac agtttttata aaagctaaca tttttattat taattgtacc
     1081 aagcgaaata tatattagct ttctttttct ctctttaggt tcaataatca actttgatac
     1141 tgatggacct acaaatgaat acttactaga gaaggtttct accattggtt gaaattcttt
     1201 tgatgtatat acaatagtat ccgtatcatt atcattagaa actatagaaa taaaattttt
     1261 tacctcatac ccttttcttt gcaaaagctt tattttttta ttaatacgtc ttatgccaaa
     1321 aatcatacgt ataatttcta taaagctttg tttcatcaac tttgctgtat gttcattaaa
     1381 ttcaaaagtt gttgtagagc atacatatgg aatattaagt ttttgtgcaa ataactttcc
     1441 ccaaaaactt aatgaatctg atacaatgca atctggatta aaatctttaa gttctctaca
     1501 aactttttca tctaaagaaa tagtagtatc tactatcatc tcaattaatc taggaaaatc
     1561 cttaccaatt tttttctcat ctccaggaag taattctgga aggtatttat cacaacaaat
     1621 aaattttgct ccagcacctt caattttatc tctaaattca ttaaaagaat aatacaatac
     1681 ttcatttcct ctatccacta attcacgaac tacttcaata gtaggattag tatgaccata
     1741 tgcaggaatg ctaaaaaata ctattttact catatttcat acctcctttt ataatttttt
     1801 aaaatcattc ttctttaggt actactccca catgacctat aaaatttatc aaatcacgtt
     1861 gatgaacaat tcctaatcct ctttcctcat ctccaagtac caataattta tctcctgcat
     1921 ttatattaaa tacttctctt gcttcttttg gaatgacaat ctgaccacgt tcaccaacta
     1981 ctactgctcc aaagaaaaac tttcctttag gaggtactat aatacctgtt ttttcttccg
     2041 aatgattaac taagtcatct agtttgacct tgtacaattt tgcaagttta atgcaactac
     2101 ctatatcagg aatagattct ccactttccc attttgctat tgattgtcta gaaacgttaa
     2161 gtttttcagc taattcttct tgtgtatact ggtggatttt tcttagactt cttaaattct
     2221 cattaatcat atttttcata cctcctatgt ttataatatt ggatttatta tttaaattct
     2281 atcaactaat cttaacattc atatatatat ttatatactt tttattagat aaatttattg
     2341 tttaggtaac atacttcctt aagttataat aaataaaagc aagcacaaat ctatcaacct
     2401 ttgctaaagt atttaagtta gggtcattta cattgttaaa tacttctaca tcttaaaaag
     2461 ttttactcat caatcaaatg aaaacttgca ataagaatta aaattataat aacaaaggtg
     2521 ctgtctcaaa ataaagataa tcttttttca ttattgaaat gataaaaata tatttgattc
     2581 cacgcaaaaa agtgtctcat gaactaaaaa gttcattttg agacactcct tttttataat
     2641 aaaacaatac tatctacttc tttttaacta tatatctagc gcagcatgat gcccttggta
     2701 tactgcattt gttattgtag atgcacgaac acaatctcca atttgagcaa catatggagc
     2761 acaatcaagt aaagtatcta ccacttctct tcttggtttt tgtccaagtg cacatattac
     2821 tgaagttcct ggaatattta ttttattccc agatttattc atacatacta caccttctgt
     2881 tgttacactt aaacccttat gttctgtata tacatatata ccttgtctct ctatttcttc
     2941 taataatatt ggacgatgcc ttatatttgc atccggggca agttctgctc tcatctctac
     3001 taagtgaaca gtcttccctt cttgagccaa gtgaattgca gcctcacatc ctgctaatcc
     3061 tccacctaaa actactactt cgtcagttac tttttctttt tctaggtaat aatcattaac
     3121 tattactaca ttttctccat taagaccctc tattggtgga acaagaggtt cagaacctac
     3181 tgctataatt aatgcatcaa cattttcatt atcaacatat tcttttgtaa ctgttgtatt
     3241 taaacgtact tcaacaccta aatcttttgc tatttttcca aaagtatttc ctaattcata
     3301 catttcatac ttaaatggaa gtgcttgttc tccctttaaa attccaccta attcattagt
     3361 cttttcacaa agtattactt tatgcccacg ctttacagct gtaatagctg cttgcaatcc
     3421 tcctggtcca ccacctgcta ttaatacttt tttagatttt aatactggaa caacttcagt
     3481 tccatctaat tcacgtccaa taagtggatt aacagtgcaa cgtcttgtag aagtggtagc
     3541 acgttctgcc atacaggtaa agcatcttag gcatttcact attttttcat cttgattact
     3601 cataactttt cttggtaatt cgtggtcagc tagtaatgca cgagccatgt aaataacatc
     3661 tgcttttcca ctagcaatta tctcttccat catttctggg tcatttaatg ctcctatagt
     3721 agcaactgga atatttacat gttttttaat ttcttctgca agatatacat tacatccatg
     3781 ctctaagaac atagaaggat gtgttactgc aaaccctctt tggtatgtac ctgctgatac
     3841 atgaagtaaa tctactcttg attccaataa tttagcaatt tctattccat aatctaaatc
     3901 ataccctccc tcaaataatt cagaaccact cattctaaac tctattggaa atcctggacc
     3961 tactgcttta cgaacactat ctaaaacttc tttagcaaaa cgaactctgt tttctaaaga
     4021 accaccatat ttgtcagttc ttttattaaa gtaaggtgat aagaattgat tgattaacca
     4081 gccatgtcca ccatgaatca taatcatctc aaaccctgct ctcttagcca atgctgcaac
     4141 atttccatat gactctacta tttcagcaat tatttcttct gtaagttctt ttatctctaa
     4201 tccatcagga cgaacactag gacttacccc ccattgtgat aatcctcgct tcttatcttt
     4261 gtcaactaaa taagttcctg aatattgtcc tgaatgagat agttctacac ttggtattgc
     4321 tccatgacga cgaattgcat ctgctgtata agtaaaactt gctaaggaat caactatttt
     4381 taagtctaga tgatatgcat gtgaaccatc tgtttctgga tgaaccatac attcactaat
     4441 tgtaactgca cccgctccac cttttgctct taattcgtaa aaggctgttg attttgagcc
     4501 aattgtacaa tctgctgtta tgtctgttcc ccccataggt gctgaaaaca ttctgttttt
     4561 gaatgttaca tttccaattt taattggctt acatagattt tgatattttc tttccatctt
     4621 ttatatctcc cttcattaaa ttttctatat ttaaattttg tattttattt atatatctat
     4681 ataaaaatgt taatataaaa cctattaaag ctataattat agctataatg aaagcccttt
     4741 gataattgcc atctacagaa tatacatttt tcatcactgc aggtccaata tatcctgcaa
     4801 aagcaaatcc aataaacatg attccataat ttacactatt atttttaggt ccaaatttat
     4861 ctactgtaaa tcctgggaaa attcccataa atgaaccaaa acatatacct ataattatta
     4921 ttccaatata gaatgttgct acatctcctt ctcttgagaa taacaaacat aataaaccaa
     4981 aaattgaaat aactaatgca cctgataaag tatttattct accaatttta tctgatatat
     5041 atcctgcaac aatacgaccg aatgtattaa acaaggctaa aattgaaact actattgctg
     5101 catctgaact tgtcatgcca atcatatttg ttgctaaaga agatgctaat gacacacaca
     5161 ttaatccata gaaagctcca caagttaata ttataatcat taaataaaat ataggtgtta
     5221 aaagcatttc tttccaattt ttattaataa taactcttac ttttcccttt ttctctggtg
     5281 ctacccatcc ttctggtgtg aaatctgctg gacatttttt gataaagaaa gaagaaacac
     5341 atacaattat aagaaatatt aaacctattg atttaaatgc agaggttact ccatatttat
     5401 taattatcca attagtaact ggaggcatta ttactgaact aaacccataa gctgctgttg
     5461 ttataccacc aactaatcca cttttatctg gaaaaaattt aattgaatta cttattgtac
     5521 atccataaac catgccaacc ccaagacctg ttattatccc atatgataaa atcaacatgg
     5581 gcaatccttt tgaaaaacct gccaatatca tacctatacc aaaaattaat cctcctacaa
     5641 atacaacctt tttaggacca aatttatcat ttatcattcc ccctgtaatc atagtaatag
     5701 gtccaactga gtttgtaact gtaaatacaa ttgctaaact agcagctgta aaagttgttc
     5761 cagcaaaact atttaaatat tctgccatag gtgatgcaaa tacactccac gcatatattg
     5821 aaccaataca taaattgatg aaacaacttg caattaatat aatccatctt ttttgtgtta
     5881 aattcattac tctcctcctc gataaattta ttaaatagaa tttcttagtt aagattatca
     5941 caattttttc tatatgtaaa tataaacatg taaatgttta catgtttata tttacataat
     6001 ttcattttta tatatagttt tatttatttt ttgttactca taattaaaaa gttcttatta
     6061 aaacatagct atattaatta attcaaacta aatatatcgt gttataataa gtatgtaatt
     6121 aaagaaagag gtgtatttat gcgaacttta aagtatgcaa ttttaggatt attaaataga
     6181 aatcctatga ctggttatga tatcgtaaaa gagttcaatt ttcaattagc tgagttttgg
     6241 aatgcaaagc atagtcaaat atatcctgaa ctaaaaaagt tggtaaatga aaagcttatt
     6301 gtatatgata ttaaaattag tggtgatgta ttagagaaaa agttatacac aatcacaaaa
     6361 aaaggtaaag atgagttctt gaaatggctt gaaaaggatg agccaatgga aagaacccct
     6421 aaggatattt ttcgtcttag aatgtatttt tctaataatt tagatataga cactaggctt
     6481 attttactag agcatcaatt gcttcaacac caagaaagaa ttacgttctt gtacagtcaa
     6541 atggagcgtt atagtgaagt tcctcctctt gatagtaatg attttggtga ttatatagtt
     6601 cttgatggag ctattatcag ggaacaaggt gctataaaat ggctaacaaa ttgtattaac
     6661 catcataaaa atttggataa tactactttg aaataataac ttattgattt taatagtgta
     6721 tttaattata tacattaaat ttataaaaaa gactgtacac aactttgtct ttgtatacag
     6781 tcttctatag ttgattattc aaaatagata attataatat ttaaaacagt taatataaaa
     6841 gttaaaactt tttattaagc tattaatttt atcttcttat tgtccttcaa aattaccttt
     6901 atatttataa tctttcacat aaaaattact atctcttctc atataaaaat ccagttgatg
     6961 tcttgttact aaatttaatg gtaatcaaat tctttaccaa taagtataaa aattactaat
     7021 gtgttataat tttcttaact actaaatttt acatacattt gaatagaata tatcaataaa
     7081 ttactataat aaataaaaaa ttaagagtta aaataaaaaa tagttaggga gatattatta
     7141 tgattttata ttttagcggt actggtaata gcagatatgt agctcgtaaa attgcacaag
     7201 aattaaacga cgaactgata tcactaaatc aacttataaa agaagaaaaa acagatgaat
     7261 taatatcagt agataaacca ttcatattcg tatgtcctac ttatgcatgg agattgccaa
     7321 tagtggttac ggattttata aaaaaaacaa aatttctagg aagcaacaga gtttactttg
     7381 taatgacttg tggaggagat acagcaaaat ccataaacta tattcaaaaa ctatgtaaat
     7441 ataaggaatg gcaattaaaa ggtatggcag aaattaaaat gccagagaat tatatagctt
     7501 tattttctac aacagataaa gaaacatcaa aacaaatgat taaagaagca gataaacaaa
     7561 tatataggat tatttctgat atacgcaatg aaaatgaatt tgaaactatt actcctagtg
     7621 gcttaggagg tacaattaaa agtggtataa taaatacagt tttttataag acaattatta
     7681 gtgccaaagg atttcattat actgacaaat gcattggctg tggaaaatgt gtagagctat
     7741 gtcctttaaa taatattaat ttaaaaaaca aaaagccagt ttggaaaaat aactgcactc
     7801 attgtatggc atgtatttgt ggttgcccca ctgaagctat tgagtataaa aataaaactc
     7861 aaaatagaga gagatactat ttagaataat atattcttaa tatttaaacc attataaaat
     7921 gaactatacc cattgtttta tacagtttta catattttaa ctgtctcatg ggtatagttc
     7981 aaaagatagt gtctcaaagt gaacttttta gttcctaaat cactcttttt ttattaaatc
     8041 aagtatactt taatcacctt ttcttatatc attttcacta atatgttgta ttccaacaaa
     8101 tataagattt attagctctg ttagtgactt ccattgtgat ttttttgaat caagataata
     8161 ataatttttc gttccttctc tacgcaaatt aacaatctct gcttctttta atatcttaag
     8221 atgatgagat acagcaggac gagaaagatg tgttttttct gtgatttctc ccactcgtat
     8281 accattaaaa tcactctcta agagagcaat taaaatcaat tgtctagtct catcacccat
     8341 agcagagata gctttgcgac attctttaaa tttagacgtt atttcttgca ttctttgttg
     8401 acattctttt tccataaaat ccaccctctc ttataagtat ctaacagttg ctacttcttt
     8461 ctgtgcagta cgtctatatt taacatctgg gtatccaatt actagtgttg taagaacatg
     8521 attactgcgt tttagaccta aaagcttttt taactttgac gaattatttg ctacatctga
     8581 aaaatatcca ctaaataaaa cacctagacc ataagactct gccattaatg ccatatttga
     8641 tgctgcaagt gaaccactaa tcttatcttt tgtaattatc attattgcaa taggagcatg
     8701 cttaaagaaa aagttatcat caatctcaac ttcctttgaa tatttaatag ctatatttgc
     8761 aataggtttt atttttctaa aaaatttcac tgcttcgttt tcataaattg actttttatt
     8821 gtctaaaaca atatatgata catcttgtga atttttagca ctaggggtat acctacctgc
     8881 ctcaataatt tgctttacaa tttctgaaga aacttctttg tctttaaatt tacgtatact
     8941 tcttctagat ttaattgcca ttagcaattc gtctgaatct aactttggct tatttgtaag
     9001 ttctatagga ggttcatcaa atcctgtcag ggtaatagct tttgtgggac agattgctgc
     9061 acaatgtcca cacattagac aatcttgtct tttaattact gactttttat tttctataat
     9121 tatattgttt actggacaat catttttaca aagcccacat ccaatacaaa gttctttgtc
     9181 aacttctatt atatgttggt atttcatatt cattcatcta ccttccgttt attggtttaa
     9241 actattataa catgtagaca ttttttatat tcattggtaa agaatttgat taccattaaa
     9301 tttttaattt ctctattaca taaatttttc atagaatttt aaaatatcat agtttatgca
     9361 taataaaaaa gacaagctta aaacctaagc ttgccttttt catcttttat tacaaaataa
     9421 attgcactta cttcccatac ttcctaacaa ttttattttt taaaatttta tctgtgaatc
     9481 atactatcca taaagtacaa tctaaaataa tcctgtgttg attttctatt ttaaaaaatg
     9541 ttaaaaatta tacttattat taatttttat actcttctta tttaacatag atttcttgtc
     9601 taatataaat cttcaccatt tgaagcaatt actttcttat accattcaaa acttttcttt
     9661 ttcattcttc ttagacttcc attaccacta ttgtctctat ctacatatat aaatccataa
     9721 cgcttttcca tttcgcctgt accagcactt actaaatcaa taggtgccca tgtagtataa
     9781 cctaataaat cgaccccatc tatagaaact gcatcatgca tagcttttat atgttctctt
     9841 agataatcaa ttctgtaatc atcttctaca taaccatttt catcagctat atctttagca
     9901 cctaatccat tttctacaat aaatagtggc ttttcatatc tatcatatac atgatttagt
     9961 gtaattctca aacctaatgg gtcaattggc catccccatt ggctgtatga aagatatgga
    10021 tttttagcag atgtaaatat gtttccttca gccatatcat ctgcattttc atcagttcta
    10081 acacaacggc tattgtaata tgaaaatgaa ataaaatcta ctgtatattt attcaataat
    10141 tcaatatcac caggttccat ttctggtgta ataccttttt gctccatata ctttaacgca
    10201 tagttaggat atttaccttt agattgtaca tccacaaaga agtaattttc tctatctaac
    10261 tttgtagctt cccatacatc attaggatta caactataag gatatacact tccagctgca
    10321 aacatacatc caattaagtt atttgaatca acttcatgtg ctattttagt tgcaattgca
    10381 ctagctacaa gttcatgatg agcagcttga tattttatct tttcaacatc ttcaccttct
    10441 tcaaagcaaa taccagcacc cataaatgga gcatgtaaaa tcatatttat ttcattgaaa
    10501 gttagccagt acttcactaa tcctttatat cttctaaaaa taactttgca taatttctca
    10561 tagaaaccta tcattttacg attacgccat gcaccatact ttttaactaa atgcattgga
    10621 caatcaaagt gtgtaatagt tactaaaggc tctattttat attttttaca ctccttaaat
    10681 atattttcat aaaattgaag tcccttttca tttggtgttt cttcatcacc tgttggaaat
    10741 attcgactcc atgcaatgct cattctatat actttaaatc ccatttcagc aaataacttt
    10801 atatcttctt tataattatg atacatatca ataccagttt tagctggata ataatgctca
    10861 tcatcaaaat caaacatttt tagtttccct gtaataactg ctgttctatc ttttcctgtt
    10921 ggacatacat ctacatttgc caatcctctt tcgtcttcat tgtatccacc ttcacattga
    10981 ttggctgcag tagctcctcc ccataaaaaa tcattcttaa aactcatagt caaagcctcc
    11041 taaaattata accaaatcac tagcttatgc taatacctca atctttacta aaataagact
    11101 gtaattagat tatcttctaa ttcaatattt ttactttctg tttctactac atctaaaaat
    11161 tgtgatgaat ttgtaataac aattggagtt acagtactat accccaactc ttttattgca
    11221 ccaacatcaa agtttaatat agtttgccct tttgtaattc tatcaccttg ttttatacat
    11281 gcctcaaaac ctcttccctc taattgaata gtatttaatc caatatgaat aagcacttca
    11341 acacctgtat cagaaagaat ccctatagca tgtagactag gaaataaggt tgtaactacc
    11401 ccatctactg gagcaactac ttttccatca ctaggtatta ttgccacccc ttgacctagt
    11461 acaccagatg caaatgctgc atcttctata tcacttaatt ttaatacttc accttttata
    11521 ggacttgtta ctatttctct ttctaattta gtagacttga cttcttttat ttcttctttc
    11581 cccttatttc ttattttttt agactcttct actttctcat ctttatataa aatcatagtt
    11641 aatacaaaag ctacgaccat tgatatagct ataccagaaa gagctactat taagttaccc
    11701 attcctccat ctggttcaat cattgctgga agttcaaata ttcccatacc tccagttata
    11761 aattttctaa gattaaataa tccgtagaaa gcgcctccaa taccactagc aatacaactt
    11821 attataaatg gtttctttaa aggcaataat attccataaa ttgctggctc tgtaactcca
    11881 aatataccag atataaagtt tggtattgcc atctctttta gttttttatc tttagtcttg
    11941 aaaaatattg ccaacacaac tgccgaactt gcaaatgtac aagcaaaaaa tggcatcata
    12001 acattatcat atcctaaagt cataacatta tttatatata caggaataaa tccccagtgc
    12061 atgccaaaaa taacaagaat ttgccaagta aaccctacta tagcacctgc caatagtgga
    12121 ctaaaatctc ttattgaaat tacaatttgt gaaataagtg tagaaccaaa tgttgctata
    12181 ggacctataa ctataagtgc aacaggcact gttattaata atgtaagcat tggcacaaag
    12241 aaaaacttaa ctaaatctgg aacaaaccta ttaaataatt tttcacacct agaagcaaaa
    12301 tacactataa atataattgg tacgactgtt ccagtataat caattgatac aattggtagt
    12361 ccaaagaaat caatatatac tggtgatgca aacattgttc ctttaaataa agtgtacatc
    12421 acatctgttc ctgctgatat actgctccct tgtatagcag gataacatac tgcagctcct
    12481 agtgctaatc ccaacattgg ttttaatcca aacttcttag ctgaagtata tcctaagaac
    12541 aaaggaagga atgtaaataa tgcatctcct gctgcattta ttaccatata accaccagat
    12601 acatcactat ataatcccat tgctacaaat aatgcattaa atcctttcaa cataccacaa
    12661 gctgccatta ttcctaacac tggctgaaaa attcctgata ttatatctat acctttatct
    12721 agtatattgc ctgatttttt agtttctgaa cttgtacttg aatcatctaa accaattatt
    12781 tcaactacat ctttatagac agcttcaaca tgatttccta taactacttg atattgtcct
    12841 ccacttttca ttacagtaac aataccttcc atgtttttta atacattgtc atttgctata
    12901 ctttcatctt ttaaagtaaa acgtaatctt gtaatacagt gtactaatcc actaatattt
    12961 tcttttccac caacattttt tactatctct tgtgctaatt gtttatattt tcccattttt
    13021 atcctccata tttcgtttaa attaattttt tgaaatttga aggtttggcc aactcaatgt
    13081 taccatccaa ccataggtat aattttgtta gtatcactcc tttcaatcat tcttaatata
    13141 ctactgaaaa attatttcac ttacatattt gagtctttaa ctactttagc taaatgtatt
    13201 gttaaacata taagctcatc atctgttaat atgtagttat attgtttttt aatgaaattt
    13261 ttaattttaa ctacacattc aaactcttta ttatatttag atttaataat gcttagtaat
    13321 tctttgtctg tctcaccttt atgtgcacta tctaaaagca atctttgtgc aaaaaacttt
    13381 aaatgcatta taaatctata ataaaacact gattcttcat gaaattctat cctaaaatga
    13441 tacctaacta tgctaagtac ttcttgtatt aacttcgtaa tatcagatgc caaagtatga
    13501 ccttcactta actgagcatt tactatatga aaggctataa atccagcctc atcttctggt
    13561 aattttatac ctgttttgtt ttcaataata tctagtgctt tcattccaat ttcaaactct
    13621 tctttataaa atcttttaat ttcccaaaga attgcatttt ttacatttat tccttctttc
    13681 attctttgaa ttgcagaata tgtatggtca cttaaggata tgtaaatact ttcatttagc
    13741 ttcttgttta actttatttg tgcatattct ataatttcac ttgataattt catgtgttct
    13801 ataggaatat tattgagtaa ttcttgtagc ttgtttgata tttctttatt tgatattcta
    13861 aatactttat ttactttagt tttatctatt agattgcctt ttgatttttg ataaccaatt
    13921 cctttaccca taacgataac ttcttcatca ttatcatcta atgtgattac aacattattg
    13981 tttaatatct tattaattat catgattatc ctccttttaa gcataaaaaa agcttatcca
    14041 taataatggg catactcctt cctaggatac tgctattatt aatgaataag cttagcttgt
    14101 acttaaacaa ttactaccta attccaatta atatgataaa acatcagaca tattttgtca
    14161 actttttatt ccatatattt catcaatctt ttgtgttact tttttgttaa ttataataat
    14221 tactatcatt aacattaact tatttcttta catcaatata gatataaaac aaattatact
    14281 caatactaat aaaaatacta ttaaagtaaa tagttgatat tatactctat ccactacatt
    14341 atctgtactt tccttaacat ttttttcaac tccaaaaaca tatgtcataa taaacggagc
    14401 aaccaatgct ataaatataa ctataagtcc atttactata tttgaaatcc ccttttcccc
    14461 tgataaacca agtactgcca ttatactatt ttgtggaaca tataaagtca catgagtaat
    14521 tcctgcaaat aaccctgcga ttcctgctcc tatagatgct aacataacag gttttttaag
    14581 tttcaatgta actccataaa gagctggttc agttataccc ataatagctg atatccatgc
    14641 agctgatgct aactgtttca tcttactatc tttgcttctt aatgctacag ctaatgtcgc
    14701 gccaccttgt gctaaattag cacaaacttg agatattaat attaacgatt cttgtcctgt
    14761 agttgcaaat gctgatatga atataggagt caatgcatga tgcatccctg tcataacgat
    14821 aaatggcatt gcagctgcta ataaacaaac cattataaat cctaaccttc cttgtagtaa
    14881 atttattata ctagctaatc cctctcctaa gaaatttcct attggtccaa ttactattaa
    14941 tgctattggc gcacttatta ataatactaa agttggattt aaaattgttt ttaacatgct
    15001 aggtgttaat ttatcaatga atttttctat atatgacatc atccatactg ttaaaagtgc
    15061 agggataaca gatgctgcat atataacacc ttgaattggt actcctataa atgatatagg
    15121 gtccccactt gatatccatg cagagaaatt tggatgtaaa aacacaccta ctactataac
    15181 tgctattgat atattaacat taaatttttt agctgcattt attgcaagta ttataggcat
    15241 aaagtaaaat gctacatctc ctattatatc taatactcta tatgtacttg aatttgatga
    15301 tagccaatta aaagtggtgc aaagagataa tataactttt atcatcccag atgctgtaag
    15361 agctggtatc ataggtgtca tacaaccagt tattgtgtct attatattat ttaaaatact
    15421 atttttttta ttttcatttt ttatatcgct tttaatatct cctgtggtat ttatcccctc
    15481 atttattatt gcattaaata caacacctac ctcattacct attataacct gatattgagt
    15541 tgaagttgag tttactccaa ctaccttatc tatagatttt aatttatctg cattgacttt
    15601 cttttcatct tttaagatga accttaatcg tgtcatacaa tgagttaagt caattatatt
    15661 attctttcct ccaagtccat caattatcaa ttgagctaca tcttcatatt tattcttcat
    15721 atcttatcct ctcttttaaa ataattttta atatagtttt aagccaaatt aatcttttta
    15781 ctccaagctt gtaatagtgt attgtaattt aataagctag tatcttctac aatatggtca
    15841 cattttccta gtacttcttt tttccccaca ccaattgacg tcatattggc agcatttata
    15901 gctttaatac ccgaacaaga atcttctatc cctacacaat catattgttt taaatttagc
    15961 atcttgctcc cttcaataaa aatatcagga tatggttttc ccctttctaa tagagatgga
    16021 tttactatac aatcaaattc atcttttatt cccaaagatt ctaatattct aggtgcattc
    16081 ttactagcag atgctattcc caatttcaca ttattttttt taagttcttt tataagccca
    16141 tttatccctg gtaatatatc tttatatgtt aatgagttta ttaactttat ataaatgtta
    16201 tttttttctt cacacacctt taacttttct tctttagtat aactatctac tttattagca
    16261 gataacaata ttttatctaa tgattcttcc ctgcttatgc cttttaattt ctcattaaaa
    16321 gtttcatcta tttctatatc tagcgatttt gctatctcat accaagcttc aaaatgatac
    16381 ttagcagtat ctgttataac tccatcaagg tcaaataaga ctgctttcat attaattact
    16441 ccttttaaaa caattattaa cttctattac attctttgat aattttattt ctttattatc
    16501 gtgtaatatg cttatgtttt tacctttgat taacttataa taagatttat attcttctat
    16561 atttacagaa actaaattat ctctaaacaa gatattaaat ttaatgtttt tccagtgttt
    16621 aggtatccta ggttcaaaac ttaatttatc attttttatt ttcattcctg caaatccata
    16681 tactaagctc atccatgttc cacccatatt agcagcatgt attccatcac ttgtattttt
    16741 ttgtaggtta ttaatatcca ttaatgctgt gtccataaaa tagttataag ctttttcatg
    16801 atttccaatt tcacttgcca tcattccaaa tattgacctt gacaaagatg aatcatgagt
    16861 agttattttt tcatagtatt caaaatctct cttcttttgt tctaagctaa actcatcagg
    16921 gaaaagcaac gctgaaagta cagtatctgc ttgtttattt acttgatatc tatatattgt
    16981 taatggatga taattgagaa gtaatggata tttttctttt ggtgtatttt taaaattcca
    17041 tactgatttt tctaaaaatg aatcgtcttg tttgcttatt ttcttctctt catcatatgg
    17101 taaatacata ttttgagcgg catttatcca gttttttact tcatattctt gtaatttaac
    17161 ttttctaaac acttcatttg aaaataaatt atgcttaaat ttatctacca tctgtactgc
    17221 atatattaaa ttatatttag ccattaaatt agtgtaataa ttattgttta ctattgctgt
    17281 atattcatct ggtcctgtta ctccatttat acagaattta ttattttttg attctatata
    17341 atctccaaat tcaacccaaa atcttgctgt ttctataaga atttctaagc cattttgact
    17401 cataaattct tcatcattag ttgcttcaaa atacgttcta ataccataag ctatatctgc
    17461 atttatatga aactgtgctg ttcctgctgg ataatatgca ctgcattcat taccatttat
    17521 agttctccat gaatataaac accctttatt tattccaagt tccctagctc tttctcttga
    17581 tgcatctaag attgaatatc tataatctaa taaagctcta gcaatggatg gttgagtata
    17641 tataaaaaat ggtaatatat acatctctgt atcccagaaa tagtgcccct catagccatc
    17701 acctgtaaga cctttggctg atatattagt ctttccatcc cttcctgctg attgataaat
    17761 atgaaataaa ttagttttta tccccagttg aagagtttta tcgccttcaa tctctacatc
    17821 agagtttgtc caaaacttat tcattttctc tctctgaata tttttgactt tatcaaatcc
    17881 aatatcgcat gcttctctta aaaccaattc taatgatttt aaatgcgctt ctgattctaa
    17941 acattttaac gactccagac attctaatga ttttaagcgt tcttcattta gatttaaatc
    18001 agaatatact ctactatagc ctacaacaat ttctaaattt aacctttcat cttttttggc
    18061 atagacttct atttctcgtt caatacattc atctgaaaca ttataggtct catttgtaat
    18121 atttccacaa gtaatatacg aatcaaaccc acaccataac cctaatttac ttctttctgt
    18181 aactaaattc ataaaatact tattgttata tttttttata tcaattttaa attgcttctt
    18241 atttatagat gccactcttg ggtcatctac tttttcacaa ttaaacttct ccatactaat
    18301 actattttcc attttatttt taaattttat ttttccatta aaattcaatg gcgttattgt
    18361 atatgaaatc atcattaatt cttctatatc ttgtgatact aatctttcta tatatacttt
    18421 aactttttta ccacgagagt tttcccatat aaatgaacgt ttaagaatac cttcatttaa
    18481 atctagtatc ctagtatgtt cacttgtaat accttttgat aaatcaaagc aatctccttc
    18541 aatagagaaa tttactatat gagcattagg taacttacag atagtctgat tttgttttgc
    18601 atagccataa gcgctttctc catatgtaat tggagattct tcataaaatc catttacata
    18661 tgtacccata ttatctatat actcattatt aaaatcagac tcttcaatat tccctctaac
    18721 tcctaaatgt ccattagcta gtgtaaacaa cgtttcattg gattttattg tatccttact
    18781 aaacccttgc tctataagtt ggtccttagt agtaaatatt aaactcataa taacactcct
    18841 ttcttaaaat gaattatgca tgagtttcca tatcattaaa taaaatttct ccatctgttt
    18901 ctattacttt tttataccaa aaagcacttt gttttttagt tcttttaaat gagcctcttc
    18961 catagtcatc agaatcaaca tgaattatgc catatcgttt tgtcatcttt ccttcactta
    19021 cactggttac atctatacat ccccacataa gatatcctag aagctctatt ccatcttcat
    19081 ttactccaag aatcatattt ttaatatgtt ctttcatgta atctatacga taatcatcaa
    19141 ttatttttcc atcctcagtt acaatatctt gagtacccag cccattttct actataaata
    19201 aaggtaactg atacctttca tataactgat tcatagttat tcttaaccct acagggtcgt
    19261 tttgccatat actatttgat tttggaagat atggattttt tatggtttct ggtccatttg
    19321 cctttgattt ttctaaacct tcagctctag ctgaactaca agcactagaa taatagctaa
    19381 aagatacaaa atctacagta ttattcttaa gtgttgataa atcttcctct tttattatta
    19441 attttatttt atttgatttt aaataataat tcataaagct tggatacaca cccctaactt
    19501 gaacatcaga aaaaaacaaa ttatgccttt catgctctaa agttgcaaat acatcttctg
    19561 gtttacatgt gtatggatat gtttttccag cagctaacat acacccaaac ttaaattctt
    19621 tatttataca ccttcctatt tctatagttt ttgcattagc taataattga tggtgtgctg
    19681 cttgatattt tttctgaagt tgattttcat cggctataaa agttaaccca gcacctatat
    19741 atggaatatg cattatcata ttcatttcgt taaagggaat ccagtatttg acatcatctt
    19801 tatatctttt catcaaaatt tcagcatatt ttacatataa attaaccact tttctattag
    19861 cccacccaga atatttttca actagagaat aaggtatatc aaagtggctt atcgttatta
    19921 atggttctat attatatttt tttaattctt ttattatatt atcataaagt tgtaatccct
    19981 tttcatttgg ttcactttct tctcctgttg ggaatattct tgaccaagat atagaaaatc
    20041 taaaacattt aaaacctatt tctgctaaca ttgctatatc ttctttgtaa ttttcacaaa
    20101 atttaatccc tatatgactt ggatagtatt gattattttc atttaaaata ttatcctcat
    20161 ctaaacatac atcttttaat ctcttatcat ttaatgggag tttatcaaat tgagataaac
    20221 cttttcctcc acttaaataa aatccttcac tttgattggc tgaaattgcc cctccccata
    20281 aaaaatcttt ccttaatttc atgcatctac ctctttcaca gtataaaatt tcataatttc
    20341 tatctcatat ttagttataa gatttttttc atactatacc ttttaaataa ttcaatttaa
    20401 aaatctaatt atacttttta tttattataa ttaatattgt ctattttcaa tactttcata
    20461 ttaaatatca atattagata tctatgtata taattatata tatagcaaat acatttttat
    20521 atcctcatta ttgttatctt attctcatat attgctgtat atttatatat ataaaaggat
    20581 ttattttgaa atagaggttg tattgagaac taaaatttag actttataaa tgaatggaat
    20641 tttcattgtt gaaatactga aaatatattt gatttcatat aaaagaaagt gcctcatgaa
    20701 ctaagttcat tttgagacac cccctttttt ctccaagcta ttatatcttt ttttaaattc
    20761 taatggagaa actccaattt tttctttaaa tactctgtaa aaagtttttg tacttccaaa
    20821 accaacttct tcaaccaaat ctacaataga tatattgtta ttaagtaata tccactttgc
    20881 tttttctaat ctatattcat ttaaaaaagt tataaatgtt tttcctgtat ttttcttgaa
    20941 aaatttagta aaataatata cactaaatcc tacttcataa gacacatctt ctaattttat
    21001 aggttgttta taattttttt ctacatattt gaatatgttg ttcaatctct ctaatatctc
    21061 tttagagttt atttcatatt ctactgtatt ttcttcaatt tctctaggaa tctgtctata
    21121 taatatagga attaaattat acattttacc ttttattgca taactatatc cttctatttt
    21181 gtataggtct tcattgtaga tatctatcaa tatatcgaaa actgacttgc ttatttttgc
    21241 tgaccaatct ctactacatt tttttatact actgaataaa ttaattaagc tttctttttc
    21301 ttcatttaat aaaattaaat cattaaaaaa agatatatca aattgaaata ctaatctttc
    21361 acttccaggt gatggcagta tataatgaat atctcctcct tttattatca ctatctcgcc
    21421 tttttttaat tgcataggct tgtcatttat aaatagattt actacacctt ctgttataag
    21481 taatatttct atttccttat gccagtgtgg tgttgttaat acttttcctt catttgatag
    21541 actacgaaaa ggaaattcat gtattaattt aggcatttct aaatatattg ccatagatat
    21601 cctctcctct ctagtgtcta aaaattaatc tatactattt taaagaatac acacctattt
    21661 taaaagcttc aaatttataa tttatacaat acctacactt cataaaatat atctaatatt
    21721 tctttttttg tatgtgcatt taaaagtctt tcacttatat ttttaccacc taaaatgtaa
    21781 gctattttag atattaatat catatcttca ttacctgcag attgtggtat agcaaacata
    21841 attattacct ttacaggtaa gccatctata cttggccaat ctacaccttt ttcagattta
    21901 cctattacta cagatgcctt ctttacatat tttgtttttc catgtggcat ggccatttga
    21961 taacctatac tagtagagcc tattttttct ctacgtttta gtgcctttat aaacttactt
    22021 tttaaagtta tacttcctgt atcgtataat ttatctacta attcctgtat tatgtcttct
    22081 tttatagttg atttcaaatc taaaataatg ctttcttctg ttaatatttc tctaatttcc
    22141 atttgttttc tccagtagat tattgtcaag taatatttta atatcctctt tattcttgca
    22201 atctattata tagtttatat agttcttttg cctatataaa tttgatattt ccattaaaat
    22261 attctttaaa aaactttgat tactaatttc taaataaata tcaatcgttc tattaacatt
    22321 attgatattc attatccctt tatttaattt actttgctta tgtatatata tatttaaatc
    22381 ttttcctaac gaaatagatt catatttact ccctaaattc acacctatta aactttccat
    22441 ctgcactaca tcaaaattaa aatatatatt atcttctaaa tacattccta ataagtttgt
    22501 atctaagttc atactttttt taggtttaga aagtaaagca ttttttatat tattaatatc
    22561 actttccatt aatagtactg atacaactat atgtaattta tctttcaaat ctaagtttac
    22621 agtagatatt ataaagtcta catcatctaa atttcgctta ctcaactgat gcataggaac
    22681 tatatcaact atagtccatt caggaaattc tctttttaat ctggctgcaa ggagctgaga
    22741 tgttccatat cctgtatgac aaactactat tactctttta ctactcatat ttctttctat
    22801 tgccgcttga aaatatgtgg caagatacga aacttcatct atactaatat tcttcagatt
    22861 atcatactct gtcataatat ttatagtcat catacataat cccagcacat ctgaaaatct
    22921 ctcttgtatc tctcctaata acgggttttt tatttggata tcatatctta atctgtttaa
    22981 cataggtctt acatgagatg ctagactttt ttgtagtaag tcatcatttt ttagatttat
    23041 atttaaaaat tgtgacatat tctctatcat tatagatgaa accttttcac tttcacttaa
    23101 tctatctgat tcatcattgt aactactatt actttcgcta ctaaatccag atgataccaa
    23161 aaaaatatat atgtacataa tttcatcttt cgttattttt actctgtatc tattttctat
    23221 tttatctata agtgtagtga catttttgta tactaactca tccaaattat ctacagaaac
    23281 ttctctttct tcctgagatt ctatttgatt accttcttcg attctcttta aacatataag
    23341 tatatgggta attaaattta tatagtaggg ctgatttata gtatatccta aattgtattc
    23401 taattctgta attatagttt ctacaaatat aatagaatct atatcaaata aatttatcaa
    23461 tgcactaaat gtaacagaat ctagtcttgt taactcagat atatcatgtt gttcataatt
    23521 ttcatctaat aaatcatcca tcatttttgc aatcgatttc cttatatcta cttctttacc
    23581 tataatctta gttccttcta aagttttatt aagttttaaa ttgtattctt ttatccatgc
    23641 ctctatatat ttaaaatcat ttactataga agttttactt acataatatt tatctgctaa
    23701 ctgatttatt gaagttatat tatttgaatt tagtaataaa tactttaaaa tctccattct
    23761 tctctgctct actgataaag atttattttc cttcgattgt atatttaaac tattaattaa
    23821 gtctatgttc ttctttgcta aatcttctat taatattcca tagcctcttt ttatatcaat
    23881 ttttatatta aaagtttcta aatatttctc aatcatcttc aaatcttttt gtaaagtttt
    23941 atcggataca tttaatttat ctttaaaata ttttataggt ctatacttat tttcgcttaa
    24001 tatcaattta agaaattcaa tttgcctcga cacaagcata cttagccctc cctatctatg
    24061 tttatatatt tctatgttta tattaagtac tattttaaca tatttactaa ctatgtaaaa
    24121 ttactaagat acttctgtat cataaaagaa tttatgatac accttactaa attgatataa
    24181 tagataatat aatttcaaaa taaaaagtaa ggatatttag aagtagttaa ctgattctaa
    24241 atatccttat tgttattcta tatactatta ttctatatac ttgttgttat tccattactt
    24301 atttttacta ttatttataa tttaagcacc cagtaattca acttcactat tttcactcct
    24361 tatagtaggc tttgttaccc ctataatcaa ggctgttatt actgttccaa tcaatataca
    24421 agctatatat gcaactggtg cactacatgc aaatggtatt acaaatattc ctccatgtgg
    24481 tgccattaaa gttatatcta aactcataca tattgcccca cctatacaag aaccaattat
    24541 atttgctgga atcattctta atgggtctgc agctgcaaac ggtattgcgc cttctgttat
    24601 gaaagatgcc cccattatag cagttggtag agatgacttt ctttcttctt tagtaaactt
    24661 atttttgaat attaacatag ctaatgatat cgctaatgga ggtaccattc ctcctaccat
    24721 taatgctgct gatggttcaa atatattact tgaaaacata gccaatccaa aagctgaagc
    24781 tgttttatta ataggtcctc ccatatctga tgccatcatc cctgctaata ttgcacctaa
    24841 taatacttta ttagttccag acatactttg taaccaagat tgtaaagctt catttaacca
    24901 tgatgctggt gtattaacta caaagaacat tatagctcca ctcgtaaatg caatcaaaag
    24961 tggacatatc aatacagcat ttactccttt taatacatct ggtaaatttt ctgttatctt
    25021 cattactcct aaagctatat atcctgttgc aaaacccgct accataccac ctataaaccc
    25081 tgaacctcca ataactgcca taaaaccagc tatcatagca ggagcaaatc cttgtcttcc
    25141 tgctattgat ttagctataa aaccagcaag tatcggtata aataacttaa atgctgaatc
    25201 gcctccaagt tgtccaaaga atccagctat agggttgtat gtttcatgag ttgggtctga
    25261 tgcaaatata ccaaacataa acgctatggc tataccaatc ccacctatta ctacaaatgg
    25321 aatcatttca gatacaccac tcattaagtg cttataaaca ccctttttct ctgacccttg
    25381 aagtatttct tttgaatctt ctaaagtctt tccacctggt ttaatttctt tagccattcc
    25441 attttcagcg tttataatta aatctttagc taataaaaca ttcttagcat ctactacttt
    25501 cttttgtaac actttctttc ctataaatct actcagttga acttctttat cacaagctat
    25561 tattacgtat tcagctcttt ttatatcttc tttagttaac ttattttcta cacctatagc
    25621 tccatgagtt tctattttaa cttcatatcc aagttcttgt cctgcttcct ctaatgcttt
    25681 ttttgccata aaagtatgag ctatacctgt tggacatcct gttatcccaa ctataagacc
    25741 tttattttga ttattactaa aatctcctga ttctgcagct ttattattaa atatacttaa
    25801 tatttcgtat ggagtttctg cacttagtag atgcttaaga aaattttctt ccattagata
    25861 tgtagctagt ttagatataa tcttaagatg ttcatctgaa cctgattctg gaactgctat
    25921 cataaaaaat gacctaagtt tttgaccatc attatctgta atctctctat ctgttcttaa
    25981 atacatcatt acaggttgat ttactacatt tgacttagca tgaggtatag ctattaaatt
    26041 acccatatta gttgatgatt ctttttcacg tttattaata tcagctataa acttttcttt
    26101 attatttata taatttccat ggtacaaatg atttgccata taatcaatca catctttttt
    26161 tgtttgtaat tgtttatcta gtacaataca atcgcttcgt agtattttac ctatcataaa
    26221 ttctcccctc cctttaattt gttacctcat aattaattct tatttttata aaagcacttt
    26281 aacttatcta tagcattgaa taaattcatt ttaaactcta taacttctct tgcaactttt
    26341 atacatggtg gatatatagt atctggctca tataagcttg ggttttgttc caatagttct
    26401 ctgcacttct tataatatgc cgacttaaca tcactcgaaa tatttatttt acaaactcct
    26461 agtttaactg cttgtgctat ttcttcatct ggatttgatg aaccaccatg aagaactaaa
    26521 ggtatatcta ctacttctct tatttctttt aataaatcta gtttaagctc tggtttaaaa
    26581 ccttttggat atataccatg tgctgtacca attgcaatag ctagtgtgtc tactcctgtt
    26641 ttttcaacaa aatccttagc taactttgaa tctgtatata ttatttcatc agtaccacct
    26701 tcacttgatg ttcctgttgt tcctattgtt ccaagttctc cttctacgga tacatttaat
    26761 ccttttgcta tttctactat ttccctagat atagctacat tctcttcata tggtaaatgt
    26821 gagccatcaa tcattactga tgtaaatcca catcttattg cccttaatat ttgttcttta
    26881 ttttccccat gatctaagtg tatcaccata ggtactttac tcttatttgc ttcttctatg
    26941 caactagcta caaagctatc tcctagaaaa cttagctcat ttgggtgtat tgcaagtatt
    27001 actggtgcat ttttttcgtt agcactctgt actactgctt taagtatttg cccacttcct
    27061 atattaaatg ctggtacagc aaattgattt tcttgtgcca cttttagcat ttccttcata
    27121 tttattaaca tattgtacct cccgatattt taaattttag gttgaatttg cttcttgtta
    27181 attttgttgt tgtttatatt taaataatat caggataatg aaggtcagaa aataacaatc
    27241 gttttcctta gtgaaaagga acttttatta tacactctaa gttccttttc actaaataat
    27301 ttttagaagt ctgaatcata gttaattata tttctcatgt acatcttctc tttttaagtg
    27361 ttttatgctt tagctttctt aactctttct aataaacaat tagataattt agctataaat
    27421 ttaaagatat tcgtatcgta gttagtacta tattggaatc aaaaaaatta aaaagatact
    27481 agatagcaga taccaattac taatacacca taagccatag atacttctat cttaaattct
    27541 tgcatattag ttacaataga aattaaatag gactattata aaaaccgtaa ggtaaggctg
    27601 acatttattc tccaactgca caaatgaata ctaatatagc tccttctaat taaatcatac
    27661 tatataaatc atatatatcc caatttttaa ccttacctct ttatatttac tttttaataa
    27721 aaaaagatat tgactaaata gtcaatatct tttagtggat ttaaatgccc acgattttaa
    27781 tctaatttct aatggtttcc ttagcataat cttataaata ttaaaattta tatacttata
    27841 aaatttctcc attaaatttt attactttct tataccaata aaaagatttc ttcttatatc
    27901 ttttaagagt cccacttcca tcattatttt tatctacata tataaatcca tatctttttt
    27961 tcatctctcc tgttgtaaat gacactgggt ctatacatcc ccaaacagta taccctataa
    28021 catcaacacc atcaatttca atggcatttt tcatttctct tatatgatta gccaaatact
    28081 ctattctata atcatcatct acagtattat ctgattttaa tatatcctct gctccaaatc
    28141 cattttcaac aatgaatata ggaagttcat acttatcata tagttgatta aggtaaaatc
    28201 ttagacctac agggtctata ttccatcccc aatcagaagt ttttaagtat ggattttcaa
    28261 ctacatcagc ctttcctgct gagttattac cggttgtttt catagtttta tcagctgtta
    28321 ctacagtagt taaataataa ctacatccaa taaaatcaac tattccttct tttaggattt
    28381 ttttatcttc ctctgtaata tctaaattat aattttttct ttcccataat tttagtgtat
    28441 ttcttggata atgacctctt acatgtacat cagtaaacat aagttgttct ctattagatt
    28501 ctacaaattt aatcatatcc tctggattgc aagaataagg ataacttggc atagctgcca
    28561 ccatacatcc aattttaaac tcagggttta tttcatgacc tatcttcaca acctttgcac
    28621 ttgctacaaa ttcataatga actgcttgat acataacttc ttctttgtct tcctgctctt
    28681 catagattat tcccgagtta gtaaatgcat aaataggatt tgtagttata ctttgattat
    28741 ttatttcatt gaaagtcatc cagtacttaa ctttatcctt gtatctactc attaccactt
    28801 cacagaagtt tacaaagaaa tctattaatt ttctattcct ccatccacca tagtttttca
    28861 ctaaatgata aggcatttca aaatggctta aagttattac tggctctata ttgtatttta
    28921 ataattcatc aaataaatca tcataaaatt tcaatccatc ttcatttgga attgattcat
    28981 ctccatttgg aaaaatccta gtccaagcaa tacttgtcct aaaacactta tatcccattt
    29041 cagcaaataa tctaatatct tctttataat tttcataaaa attaatggct tcatgattag
    29101 gataatttaa gccctcaatt attccatctg ttatctttct tgaaatagta tgagtacctg
    29161 cagtcatcac atctgcaata cttattcctt ttccaccttt attatatcca ccttcaactt
    29221 gatgggcagc aactgcccct ccccataaaa attcttgatt agatttcata gaaatatctc
    29281 ctccagtata attaataaac aacttttatt acaggcgttc caattttaat atgtttattt
    29341 tctttaacta ttacatctgc caaatcattt gtgttggtta gtattattgg tgtaacaata
    29401 ctataccctt caccttctat tttatcctta tcgaaagaaa caatcttatc tcccttttta
    29461 actttatctc ctgcattcat atgagtagta taatactttc catctaacat agtagtgtta
    29521 agacctacat gtattaatat ttctacacct ttatcagagg taattcctat tgcatgcttt
    29581 gtaggaaaaa tagttgttat tgttccattt acaggagaca caacttctcc ctttgaaggt
    29641 attattgcca tgccattacc tatagttcct gatgcaaaca cttcatcttc tacttcagat
    29701 aactctattt gtaagccttc tattggagat aatatttcct ctataccatc atcttcatca
    29761 aaagcaactt cttcattacc ttcttcacat tcttctttac tcaatccaaa gaaatatgac
    29821 aatactgctc ctagcactaa tgctgttcct attcctaata tatatcctag gaatggtgta
    29881 taaactggca ttgagaatat attatggaat acatatgcat tacaagtaac gccaaataca
    29941 ccacatatac caccaccaat acctcctgca atagccaata ctggaatcat tcttctgtat
    30001 cttaatataa ttccataaat tataggttct gttactcctg ctaaaattcc tgtaactgct
    30061 gttgaacctg ctaatacttt taattctgaa ttttttttag attttctaac cttcataaat
    30121 aaacctattg caatacctat ttgagcaaat actgctgcaa cagctgctcc ttctattggg
    30181 tctccaccca ttgtagccaa gttttgtaaa gtaattggtg tgaagcccca gtgtaatcct
    30241 aatactgcta agaatggcat agctatacct attgcagctc ctgcaattac tctgttaaat
    30301 tcaaataatc ccataaaaat attggatatt gcatcaccaa cattagttgc tatcggtcca
    30361 aataaaatca cagttaaagg aaccatgact aataatgata tcatcggtac taagaataat
    30421 tgtaaatctt ttaatataat cttctttaaa agcttatcaa catagtaata aataaatata
    30481 gcaacaaata tagggaatac accaaaagaa taatcaattg ctgttactgg catcccaaaa
    30541 aagttagcac caccttttgc tccaattaaa gctgtaaaat taggatctaa caaagctgct
    30601 cctatcactc caccaacata tggatttgca ccaagtttta tagctaaagt aatacctaag
    30661 aatattggta aaaagtaaaa tacagcattt ccagctgctg ataatatagc ataagtgcta
    30721 cttgtatcag acatccattt catagtagtt agtattgtaa gcagtgcctt aatcattcct
    30781 gctccagcta gtgcaggtat taatggagaa aatgcacctg aaattgattc aaatatttta
    30841 gaaactattg aaactttttc tccctgttct tcagtatttt gtccttgtaa aactgctttt
    30901 tttataatag tttcaaagta atcagttact ttatttccaa ttaccacttg gtattgtcct
    30961 ccactattta ctacagatag tacatccttc atttttaata actcttcttt gttagttttt
    31021 gaagtatctt tcaacttaaa acgtagccta gttgcacaat gaactaaact aataatattt
    31081 tcttttcctc cgacatattt tataatttct tcagactctc tttcaaattt catacaatct
    31141 atcctccatt ttcataattt tatgtataaa aatagaccta aatcaataca attagatatt
    31201 aaaaactaat tataccaact taggtcatgc ctgccaaaca gtaacaatcc tacactattt
    31261 actttatatt tgataatcta tttaaatgca atgttaagta caacaattca ttttcgctaa
    31321 ttttccattt atacgtcttt tcaagaaagt tttttacttt ttttgcacaa ttgtaatcac
    31381 ttttgtattt catttttatt atatctaaca atgatgtgtc tacatttgta ttctcaccac
    31441 ttaattgcct ttttataaaa catcttaaat gacttataaa ccttgtgtag tcaaaggaat
    31501 attcatttat ttcgattcca taatgatact ttatgatatc taatacatta tcaattatct
    31561 tagttaacaa taaagtttct tgcatatctt ttgtttctaa ctgagcattt gcaaagtgta
    31621 tcgtaataaa tgctatttca gaatcaggaa tatctttatc taattttttt cttaaatact
    31681 ctacagcttt cttactaaag ttatattcat taggatatat ttgtcttatt tcccattgaa
    31741 gtgggctttg aaatactaat ccttcatcca ttctttttaa catataactt atatgttcgg
    31801 ataatgtgat taatatacta tcattaagat tatcactcaa ttcctctctt cccatttgaa
    31861 taatttcact tacaagctca acatctttta aaggtatcct atctagtagt gaatgtaatt
    31921 cacttaatga ttcctcacta ttgaatataa atttcttttc aattttatct atttcaacta
    31981 aatctttagt aaatttatta aatccaattc cttttcccat taatattact tcttgtcctg
    32041 aattatctct ggcaaataca acattattat taaatacttt ctttataacc ataaactacc
    32101 tccactgtaa acaaaaagag acctaaaaaa gactaataat actttaagca tttactagaa
    32161 tttttaggtc acgcctgatt acatctatct cagtaacgat ccgtttattt atctgtatga
    32221 taactttatt ttcattataa attaataact gtacttagtc aatatataat ttcatgtata
    32281 tctctactag attattgtca taggtattca ctcattctac tttttttata tgatttatat
    32341 aatctaaaca tgctaaaata agttttgaag ttatttcata acaaaattaa gctcatatat
    32401 tttttattat atatactcga ttatttatcc atatataagg aaatccttta tttttgtaac
    32461 tacaataact gcctaagttt tgattttctt ttatctttta cttctcctta ttaaaaaata
    32521 attcttttac tgttgtatct aagtctctaa acagtttaat tatcaaatca ttgtttggat
    32581 tattaacctt acctaactta ctatatagct ttttaactgt atatctttaa tagacctttt
    32641 ggtatgctaa tccattttat aacattaatt cttgaatcaa gcatatgtta cccttacaaa
    32701 gcatatctta taccctctgt aaagttatga tagttgatga acctgaagaa attaccctta
    32761 caagacatat ctgatacaac ctcacttata agtttatatg tttcagaagt atataaataa
    32821 cccatacttc cttctaaacc cttacctgtt ttcttagaaa attctaatat atttccttac
    32881 atagcaattg taacagtatc ttattaaatc ttataaaact atacatcctc tttacattta
    32941 aactttaaac ttttatctat atgtttatta attaactgta cattcactat tttttatcat
    33001 ttataatact cttcttattg tcttaaaaca attataatct aaccttaatc acatattata
    33061 taaatatttt taacaaactt aataattaaa taactatatt tcattatcca tcttataaat
    33121 gtttataaac tttccactat tcttcttcaa taaattcaaa caaattttct actgtggtat
    33181 cgaatacttt aacgatatcc atagctaact tcaaagaagg attatatttc ccattttcta
    33241 agtgtacaat agtctcacgt cttaccccaa ctaatttagc aagttcattt tgagtcataa
    33301 ggagtttttc tctgtattct ttaatttttg ttttcatcaa tattgccact tagttacata
    33361 tccccctttt atcaaagtac atatacaaca ctaatttaga taaagactgt ataaataaaa
    33421 ctgcaatgag gattatacct gtatctatgt ttgatatatt tatattactt gtataaggtg
    33481 ttgcaaagaa aatagatatt attactatag aatatatcat tatttttatt gcaattttat
    33541 taacctttga tagtataaat tcagatgatt catcaactaa atcattatat tttttattat
    33601 aaattgaata acatccaaaa actattagcc aactcaaccc tggaagagaa gcagtgcttg
    33661 gtcctttaaa aatatactta gtggctaata cagctataaa aaaataagtt attacaagca
    33721 actccaggat aagctttgat tttacaccag tttctgattt agacatataa aatcactcct
    33781 taaaaattat attctctata ctctttaatt ttcgttttta tcaatattat cacttagttg
    33841 catattccct ttttatcaaa atatatatac aaaattaatt tagataaaga ttgtataaat
    33901 aaaatagcta agagaattac acctatatcc aaatttgata tatccaaacc tcttgtataa
    33961 tttgctgaaa agaaaacagc tattagaaaa atagaatata acattatttt tgatgcaata
    34021 tgatcaacct ttaataacat aagttttgat gcctcgtcaa ctaaatcatt atatttttta
    34081 ttatacatga agtcacatgc ccaaaaagcg aataaaatta taaaagtaat ccaaatattt
    34141 ccccctttaa aaataaactt ttctaataat gagtctataa aaaaatagga catcataagt
    34201 aactctgaaa caagatttgt tttaacacca agctctgatt tagacatata aaatcactcc
    34261 ttaaaagtta tatatttata acacctcatg ttataaatat ataacttttc caaataaatt
    34321 gcaatagaat attgaaaata ttcaataata tctttataaa tatagtttat aaaagatttt
    34381 ataataaaca ctacaataca gtactaaatt aatttattgc atctattatc tcaatcatat
    34441 catttactaa ctattattct aatgagcata taacattgaa atttataatt atacaaccta
    34501 aatgcatccc aatgtaccct aaaaacctca ttttctgtaa ctgaagtata attggtagat
    34561 tttatcactg aaataaaaaa cactagtcaa taaagactag tattttcaag tattttatca
    34621 ttcttttttt aaattattgg agacgaatca tcagaataat acccacaatt tatctatatg
    34681 caaattctaa atttaaaata aagtttcaga tgaatttact attctatgct gtgatacaac
    34741 agaaaaaact tcaatctata agtggcagtt tcatatagtc ctaattccta caataaacag
    34801 aaaggaggtt tattctatat ataactaatg ataaatttta ttataggagc tattagctct
    34861 atttgtgttg ctataagtac taatta
//
LOCUS       DAEMAE010000014        32107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEMAE010000014 DAEMAE010000000
VERSION     DAEMAE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.014"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..782
                     /locus_tag="KPH93_000602"
     CDS             <1..782
                     /locus_tag="KPH93_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG1687439.1"
                     /translation="LIYSNMIDKKTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQV
                     KILQNNINISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFIN
                     SSKKFTGDSIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSAIPAKDTDDSIKIAEQIK
                     NEWKKRKISFKNKKNISIVNGFLRYPETKNVFIGHTGICIKTKNGILFLEKYGVTSPY
                     QVTKFKNKKDVKNYMFNRLKMSEGEIELPDPIIMENDKLMK"
     gene            complement(1095..1874)
                     /gene="hisJ"
                     /locus_tag="KPH93_000603"
     CDS             complement(1095..1874)
                     /gene="hisJ"
                     /locus_tag="KPH93_000603"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG1687440.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAIDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKINNNYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDTI"
     gene            complement(2420..2770)
                     /locus_tag="KPH93_000604"
     CDS             complement(2420..2770)
                     /locus_tag="KPH93_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG1687441.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2786..3202)
                     /locus_tag="KPH93_000605"
     CDS             complement(2786..3202)
                     /locus_tag="KPH93_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG1687442.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3221..3412)
                     /locus_tag="KPH93_000606"
     CDS             complement(3221..3412)
                     /locus_tag="KPH93_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG1687443.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3563..4378)
                     /locus_tag="KPH93_000607"
     CDS             complement(3563..4378)
                     /locus_tag="KPH93_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG1687444.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDKELTYSIIIGEDNPLIDK
                     SINMSYRYISEGLVKGLNLSGIKTDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RRIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLAKELYEKYKNEEYNKKR"
     gene            complement(4425..5363)
                     /locus_tag="KPH93_000608"
     CDS             complement(4425..5363)
                     /locus_tag="KPH93_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1687445.1"
                     /translation="MIRLSVGTAIELEILDKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKTYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGIGESHEDIYNLYTYLKENDITISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKECFKFKNGYLDSIKLDNDIVKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5360..6232)
                     /locus_tag="KPH93_000609"
     CDS             complement(5360..6232)
                     /locus_tag="KPH93_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1687446.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKESIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIR
                     EHTEVAPHICIGLKGGQIKGEYEIVKYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTITECKECCVL"
     gene            complement(6257..8185)
                     /locus_tag="KPH93_000610"
     CDS             complement(6257..8185)
                     /locus_tag="KPH93_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG1687447.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8297..8674)
                     /gene="gcvH"
                     /locus_tag="KPH93_000611"
     CDS             complement(8297..8674)
                     /gene="gcvH"
                     /locus_tag="KPH93_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG1687448.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8761..10887)
                     /gene="cdhC"
                     /locus_tag="KPH93_000612"
     CDS             complement(8761..10887)
                     /gene="cdhC"
                     /locus_tag="KPH93_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG1687449.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVVTDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10944..11750)
                     /locus_tag="KPH93_000613"
     CDS             complement(10944..11750)
                     /locus_tag="KPH93_000613"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG1687450.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11800..13167)
                     /locus_tag="KPH93_000614"
     CDS             complement(11800..13167)
                     /locus_tag="KPH93_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG1687451.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDSKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSELEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRIALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13198..14142)
                     /locus_tag="KPH93_000615"
     CDS             complement(13198..14142)
                     /locus_tag="KPH93_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG1687452.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14162..14932)
                     /locus_tag="KPH93_000616"
     CDS             complement(14162..14932)
                     /locus_tag="KPH93_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1687453.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14988..16373)
                     /gene="lpdA"
                     /locus_tag="KPH93_000617"
     CDS             complement(14988..16373)
                     /gene="lpdA"
                     /locus_tag="KPH93_000617"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG1687454.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16417..17298)
                     /locus_tag="KPH93_000618"
     CDS             complement(16417..17298)
                     /locus_tag="KPH93_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG1687455.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17334..18008)
                     /locus_tag="KPH93_000619"
     CDS             complement(17334..18008)
                     /locus_tag="KPH93_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG1687456.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKNKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18045..18917)
                     /locus_tag="KPH93_000620"
     CDS             complement(18045..18917)
                     /locus_tag="KPH93_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG1687457.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19009..19641)
                     /locus_tag="KPH93_000621"
     CDS             complement(19009..19641)
                     /locus_tag="KPH93_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG1687458.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19746..21422)
                     /locus_tag="KPH93_000622"
     CDS             complement(19746..21422)
                     /locus_tag="KPH93_000622"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG1687459.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLERLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21671..22450)
                     /locus_tag="KPH93_000623"
     CDS             complement(21671..22450)
                     /locus_tag="KPH93_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1687460.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22750..24669)
                     /gene="cooS"
                     /locus_tag="KPH93_000624"
     CDS             complement(22750..24669)
                     /gene="cooS"
                     /locus_tag="KPH93_000624"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG1687461.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTNELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25276..26187)
                     /locus_tag="KPH93_000625"
     CDS             complement(25276..26187)
                     /locus_tag="KPH93_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG1687462.1"
                     /translation="MIKKLDDILDQLRGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMANSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26303..26881)
                     /locus_tag="KPH93_000626"
     CDS             complement(26303..26881)
                     /locus_tag="KPH93_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG1687463.1"
                     /translation="MNINEFLTHVNQRKPVFSKSEIHEFMVRLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGVAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            27106..28029
                     /locus_tag="KPH93_000627"
     CDS             27106..28029
                     /locus_tag="KPH93_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1687464.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIAKTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYTQI
                     PATDVWGLLMRKDSALASQNTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLSIV
                     ATYNLVYNASLLVEEGLGYALCLDRLINTTENSNLCFRPLSPPFEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(28201..29343)
                     /locus_tag="KPH93_000628"
     CDS             complement(28201..29343)
                     /locus_tag="KPH93_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1687465.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(29361..31115)
                     /locus_tag="KPH93_000629"
     CDS             complement(29361..31115)
                     /locus_tag="KPH93_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG1687466.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVSMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNMPQFFNKLDMDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            31251..31457
                     /locus_tag="KPH93_000630"
     CDS             31251..31457
                     /locus_tag="KPH93_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687467.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(31462..>32107)
                     /locus_tag="KPH93_000631"
     CDS             complement(31462..>32107)
                     /locus_tag="KPH93_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687468.1"
                     /translation="KYVIYFKKEKLLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSI
                     KDKIIGFSIELDKSDVVRFQMARIKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVY
                     MEDRTVENEGINSIKKSILSILGEDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNK
                     SIDPRSLIRLEEGNTIIDPIFNQITVMSKTFNDNILSIKINSKSGIYILKFKKV"
BASE COUNT         9797 a         6361 c         3633 g        12316 t
ORIGIN      
        1 aattgatata ttctaatatg atagataaaa aaactcaaaa tagtgtaaag gaaatcctaa
       61 aagaaaataa aattaatgaa aaagacatag atacatttat taaagcagtc aacaattata
      121 ataaattgca agttaaaata cttcaaaata atataaatat atctaaatct ggatattctt
      181 caatcaatgc aaaacaagtt ccatataacc tagaaaaatt gcaagataat tgggtgaaaa
      241 aattccctga ttatatggat gtaaattgta gaataactgc ttttagactt tttaaagatt
      301 ttataaattc aagtaagaaa tttactggag actctattga tttgaatgtc gatttggaca
      361 ctataatgaa taataaagat gctaaattta gcaccaaaga tgtagaaaaa tttatcaact
      421 tcttttctgc aataccagct aaagatacag atgattcaat caaaattgca gaacaaataa
      481 aaaatgaatg gaaaaaaaga aaaatatctt ttaaaaataa gaaaaatata tctattgtaa
      541 atggatttct tcgttatcca gaaactaaaa acgtatttat aggtcataca ggtatatgta
      601 ttaaaactaa aaatggcatt ctatttttag aaaaatacgg tgttacatca ccttatcaag
      661 ttactaaatt taaaaataaa aaagatgtaa aaaattatat gtttaataga ttgaagatgt
      721 cagaaggtga aattgaattg cctgacccta taataatgga aaacgataaa cttatgaaat
      781 aataacttta aacgcaaaat agtatttata attactaagt caatataata gagaaggtct
      841 atagatttgt gtaaatacaa gcttatctac aatcttatat aagaatgttt atataaataa
      901 gatagattaa ttctaagtcc tattattcta ttgaatttta ttattatttt acttagattc
      961 tattgttatt ttacttagaa cttattatta ttttatttaa gttctattac tattttcaat
     1021 catataaatt tacaaaagac tgtacataaa tttattttat acaaacttat ctacagtctt
     1081 ttgtgtgaat atatctaaat agtatcgtat ttatttattt cctctgcaca gtgagaatca
     1141 gaacctaaaa ctaatggaat atcgtatttt tttaccaact ctaataagta tccatctata
     1201 taaggctctt tgcagtactc ttttctattt ccagaaacat tataatcaag ttcataacct
     1261 ttttctttaa ttaactttat taagtcttca agaattgtat ttcctttata atcgtatgga
     1321 aatacctgat taaactttct aactaaattt aaatgaccta ttctttttgg tttatgtata
     1381 cccaaatctg aatttacagc ttttatcaat gtttcataat acttattata aacagcttca
     1441 atacttccta acttgtcgat taaattttga aactcctcta cactgtaatc tacacaataa
     1501 taattattat taatcttaat tatatgtact gataaaagag aatcctctaa ttcttctcca
     1561 tatttattta aattcgcctt tattccttct tcgtatcctt ctataaaatc cacttctgac
     1621 ccaacattta tcttgatttt accctcatac ttttctttca acttcttgac atcattaaaa
     1681 tactttggca aatcttcaat actcatacaa ctatcatttt caggagcagg gtctctaaat
     1741 ccatttgcat acggaaaatg ttccgtaaat gtcatttctg ttattccaac atcaattgct
     1801 ctttgtacat atttttcaaa atcatcctta ctcccatgag gacaataagg tgaatgtata
     1861 tgaccatctt tcataaataa ttcactcctt ttttaaagaa aaagtctaat ataaatacat
     1921 aaatctttta acttcaaaac aatattttta attatactaa ttcttattat atctaatatt
     1981 agagtaattt taaactgcct tttaagtatc ttatcattag tactattttc aacttaacat
     2041 taatattatt ttttaactta acattaatac tatctttaaa tagtaatact caatatataa
     2101 gttacttaac atcaaataac tcaaattgct tgatgaggct tttgcaatat ttaaagaata
     2161 tataattatt taacattaaa taatccaatc ttgtaataaa atttactcat caaagtatat
     2221 tacagaaaat gcctcaaaat aaacttttta gttcatgaga ctctcttttt gcatgaaatc
     2281 agatgtattt ttatcatttc aacaataaaa gggaggctta tatccatttt gagacagccc
     2341 attagataaa atttttgata attaaacatc acaaaacata aaaatcgctt atatcaaacg
     2401 tataagcgat taattatatt tacatatgtt ttttagcctc taacaacatt ttattagctt
     2461 cttccatata ctctatagcc ttttcaatac tctttgctgt ttcttctttt ccaatagctc
     2521 tagccttttc aacccactct ctaaatcctt cttcatgagt ttcattgtga tttatccaat
     2581 gtactaaaag tatttttaaa gttttttcat ccttagactc tgatgattcc tcatgactat
     2641 gttcatggtc gtggtcgtga gcatggtcat gtggatgttc atggcatata tctccatgag
     2701 tatgttcgtg gcagtggtca tggtcatgat gtccatgatg atatcttaca atctctttat
     2761 caaaaatcat tagttattcc ccctgttatt ttatattaaa ctcttttttt ataaggttta
     2821 cagaatcttt tacaagtagt gctaatggtt taccttctaa tccaactaac tccacatttt
     2881 ctggcataat aggtaaaagt atcttaagtg cctcactgtc cgcaatagct tcacttatat
     2941 tacaagtaac ttctcccatc atagaattag gtattattac agaaataggt gcaactatta
     3001 tatttgcttt tttagctgat actactatag cattttcccc tgtagctcct ttattagcat
     3061 gagccttcat catagaacta gttgctatag aattagtccc tagtgcataa atttctacat
     3121 atgtaggtaa ctcttctctc aaagaagaaa ctatctgggc tccaatacct ccacccattc
     3181 catctatcac tgctattatc atatgaaatt cccctattaa ttaattttct tgtattatta
     3241 tcttatggtc catcaatcgg atttctttaa ttatcccgtc tattatcttt tgctctccta
     3301 ataaatctgt taaataaatc tttccatcac aagggtctat attaaccaca ttttccataa
     3361 ccttttctaa ctcattatta cttttacaaa tgaaagctga tgattcacac atataaatct
     3421 cctcctaata tgatttttat taaaaatcaa aaaaagattt ccttaggtat ttcaatacac
     3481 caaagaaacc ttcatgattt cagtttttgt atattattat accttaagta tatcacttta
     3541 atttatataa agtcaattaa agttaacgct ttttattata ctcttcattc ttgtattttt
     3601 catatagttc tttagctaat tgcttttcat aatcagtcaa atcgccctcc acaaactcaa
     3661 cattaaattg ttcagctaag ccctttacta tgtttttctg tagaatatct atgtctattc
     3721 ttctgccaat ttcattttct attccacttg cttttttagc tgtaaatttc atagcccttt
     3781 ctttcagttc tggtgtctta gtttttatta atttaaacaa tttttctaca tcaaaatcca
     3841 gtattattga accatgttgt aataaaacac catcttttct gctttgagca cttccaatta
     3901 ccttcttatt attaatagta acttcatatg atgcatgtgc attaaaacat gcagctgata
     3961 aattttccct acttattctc tcgcctctat ttaaattatc tgtttttata ccacttagat
     4021 tcaatccttt aactagacct tcacttatat atctataaga catgttgatg ctcttatcga
     4081 ttaaaggatt atcttctcct ataattatac tataagttag ctccttatca tgtaatacag
     4141 ctcttccacc tgtaattctt ctagtatagt ctatattcat acatctacac ttgtccaaat
     4201 ctatctcatc ttccaatttc tgaaaatatc ctatactcaa acaagctggt tcccatgtat
     4261 aaaatctaag agttggctta ttatgtccct tcttatatgc agtaaatata gcttcatcaa
     4321 ttgccatatt cattgctcct tcatatgatt tattatgtat aactctccac tgattcatga
     4381 ttattacgcc cccattttta gtataaatat atcctagaaa tcctctatag atttatttct
     4441 cttattgcta aatctatctc actttgattt aaaggtcttg aatagttata tatagtactt
     4501 cctggtcttt cattatagta tggtctatta caatccttac aacctcttat ttcaaaagga
     4561 tagcctttgt ttatgtcttt tacaatatca ttgtcaagct ttatactatc taagtatcca
     4621 tttttaaact taaaacactc ttttggataa cctttattta tcatataaga cattaactgt
     4681 acccttctat aactttctat gcttggttga tttatctttt ccattttagt tcctctaact
     4741 ggagtaaatg caaataaact aatcgttata tcattttcct ttaaataagt atataaattg
     4801 tatatatctt catgactttc acctatacca actattatat gtgtacttat tttattggga
     4861 tatgactttg acatttcagt tataaatttt aacttttcat catagttatt tccttttatt
     4921 ttttcatata gttctttatt tgctgcatct attgctattc ctattttatc tactcctgct
     4981 gaaaaaaact tctttatctg ttcatcattt tctaattttg cagatagtga aattggcatg
     5041 tctatcttac cacttatata gtttataaaa tctaaaacag agttatgtgc ttcttcactt
     5101 gccattgatt gaatacatat tctctttata ttttttcctt tataagtttt taaagcatct
     5161 aatatttctt cttttgaaaa ctctggccaa attactcttg ataatttatc ttttctagta
     5221 ctactttcta ttgattgaga acaaaaactg catttattta tgcatttttc tccaatcatt
     5281 atatatgctg tggtaggagg tatatcactt tttttgtcta atatttcaag ttctattgct
     5341 gtcccaactg atagtctaat cataaaacgc aacactcctt acactcagtt attgtcatat
     5401 tcatttctat agccttattc tttgcagacc ttgatggtaa tactattcta ttgactccac
     5461 acattataga taactggtct agctcttttc tatacactcc tcttggcctc atacacccta
     5521 gattaatttc agtatctggt aaattaattc tagcttcaca taaaatatct gctacccctt
     5581 ctaactcagg aggttctaca ttttcatact cagtcccttt tgtgggaata agtactataa
     5641 atgtaagctt atttggagga tttttctgta ggtattttac aatttcatat tcacccttta
     5701 tttgtcctcc ttttaaacca atacatatat gtggtgctac ttctgtatgt tcccgtattg
     5761 tattataaac ttcaatataa tcttctttac tctttttcat cttataaact tctcttattg
     5821 tctcatcatc aaacactaaa tcaaaagaaa ctatatctaa atacttacac aactctacaa
     5881 tgctctcttt atccataaga cctaaatgag cattcaatct atatccttgc tcttttaagt
     5941 ttttaatagt attaatatga gtatttatcg ggacatctcc ttcataacta caaccgccac
     6001 tcaatagaaa gctagttata ttccttgatt gtactttttc ctcatattct tctattggca
     6061 ccatattgtt taagtagtga ccgttgcaat gggcacattt caaactacat tgattgcttg
     6121 tagtactaag agctagtgtc tggttaggat aagcaaactc tattttattt ccaaaattct
     6181 ttaatttaat atcaaatgct attttcattt tttcagatag attcttattc atagtttatt
     6241 ccttcttaag tacattttac tcttctagta atttctttgc tgttgggaac tgttctatat
     6301 tcgtatgtgg taagaaacat gcagaaataa attcatccat ataacttgga tatacactaa
     6361 gttcaacata agttatttga ttaccgattt cttcaagttt attttttgca tctttactta
     6421 tgagagctaa gtaagaacca acaagtgaac tattccctat ataagtaaat ttttctctat
     6481 caatatcagg aagtaaccct ataattattg agttttctat gtctaggttg tttcctatac
     6541 cacctgctat ataaactcta tctaatatac taaaatccat acctaaacta tctacaagag
     6601 tgtatgcacc tgaatatata gctcctttag ctctgatgaa gttatctata tcaacttcat
     6661 tgactactat atcattttct aaatcaaatt cttctttaaa tgctaataca tactctccta
     6721 tctcatgctc attaaatcta actctcttat tatctaaatc cctgtatatt ttacctcttc
     6781 tatctattac acctttagta atcatttgac agattaaatc aattatacca gaaccacata
     6841 taccaactgg tgcacattca tctataatct ttagtgttgg ctctaaagtt tctttatcaa
     6901 taattacttt ctctattgct cctgctgatg ctctcattcc acagcttatc cctccacctt
     6961 caaaagcagg tcctgctgaa catgcacagc tcatcatata atctttatta ccaaatacaa
     7021 tctctccatt agttcctaaa tctataaata aaacattttc ttcacttgac catatccctg
     7081 ctgataatac acctgctgtt atatctcctc ctacataact tgcaacagat ggagctaagt
     7141 acacatatgc actgtcattt acgaaaagac caacattttc acccataagt tttggagatt
     7201 tcaaaaatgg tggtatgtaa ggttcttgtc ttaagaagtc tgtatacaca cccaagaata
     7261 aacttgtcat agttgtattc ccagcagcca ctaatgtaac tacatcttcc ttatctattc
     7321 catttctttc atatattgtt tttaatagtg gatttatagt ttcctcaact atagctttat
     7381 gaagtgtctc taatccattt ttcttagttg aatatataat tctatttata acatctgctc
     7441 catattttat ctgtgcattt ccagaagatg ctttatctac tacctctttt gagtataggt
     7501 ctactaaaca aacaactact gaagttgtac caatatctat tgcaactcca tataatgaat
     7561 tttctttatt tccttgttct atatttatga ttgtaagttt cttttgtttt tgaacatatg
     7621 ttattgtaac cttaaaatca ctctttctaa atacggttgg catctttcta agtatatcta
     7681 atctaaaatc tatttcatta taccctaggt tatttctaac atatctttcc aatctatcta
     7741 catcacttat attatcatct aaatttggtt cttccatttc tatgtatttc ttttttatat
     7801 ttgttttaaa ttgaagattg tgttcttcaa ttattttttt agctctttca aaaatttccc
     7861 tatcctcttt tttattacta ccttctatct tcataccatg catagaagat gatactttag
     7921 aaggaacttc aatttctata tctgatatca cctttgtaca acaagctagt atataacctt
     7981 gctcccactc atcatctgtt atatgtcttg ttttttctgt atcaactttt ccattcaata
     8041 attttacttt acacttccca caagacacgt ttccattaca aggagcatct atgaatatat
     8101 ctgcatttct tgcaacctct agtaatatat ccccctcatt acaataaacc tctttattgt
     8161 ttggtgtaaa acttactttt atcatgttat ccctcccaat ttgttctata tatgataaaa
     8221 ggaaagtata gctaaatttg cgtgcactca aattttacta tactttcccc aattataata
     8281 atatcttaat ctttaattat tctaatccag cctcgtattc tttatcactt aataacgtat
     8341 caagttcagc cacatcagct agttttactt ttaatatcca gttttcatat gggtcttcat
     8401 ttatacattc tggctcatct tctaattttt cattaacttc caatacctca ccattaacag
     8461 gagatattaa atcagaagct actttagaag actcaactac tccaaaatct actcccttag
     8521 ttacagtatc ttctacttct ggtgtttcaa caaacaatat ttctcctaat tgatcttgag
     8581 catagtctgt tatacctata tacacaacat ctccatcaat tacttttacc cactcatgat
     8641 ctttagagta ttttaattct ggtaataatt tcatttcgat agacccccaa tattatatta
     8701 ttgattaaat tacttaaatc atattaatat tatatcatat tattttaaat tttccatcaa
     8761 ttacattaca ctttccatag ctaatgctgg atgacctttt tcttctaaga aagctaatac
     8821 tccttcagaa tcgtcagcta tagtttcatc acaaaccata tcacagaagt tttctattcc
     8881 agtcatttct ttaactgtag catttaattt atcagctaca taatctttta attcttttgg
     8941 catccaaact attctttctg gtccaccttc tgcataagcg aattttttag aagatatgaa
     9001 atgtcttccg tgtcccataa atcctggagt ttgaactcct ccacctgtca tagaagcaag
     9061 ttctccgaaa gtcataccaa ctggagtaac acttgcaaat tctctgttaa ctacaacaaa
     9121 tccatttgct tctggcatta taccacagat acattcgaag catccacaag aagtcattgg
     9181 gtcttctagt atagagtata atgtaacaga ttcaactgca ccttgagata tttggtttac
     9241 agtttcattt actgaattcc atacacctgt tctatcatct aagcactcgc ctttttctat
     9301 tggttgacaa ggacctgttg ggtctaattc tttagtagcc ttagcatcta accagctaac
     9361 tgctccacaa agaccaagtc tttcaggagt aacaacacaa acatgagctg gtgcaaatga
     9421 ttgacataag ttacaagaat agaaagtatc aacactttca tcaactaatg aagctaatct
     9481 ttcatctcta gcattgtact tagctattgc ttcacctttt aattcagaaa ctttttcagc
     9541 atctgtaatt atagttattt cacacttatc aactactgat tcaaattcat ctaacatctt
     9601 agcatataaa acttccccta tatgctcaag tctaaatcct ttatcaaatg catctttaga
     9661 gattcttacc caagccatat ctctttgtcc aacgtgcatt acaccttcta tatagtttaa
     9721 gaagtagtgg aaacgtcttt caagaactgg ctcgaaatct tcttgcatat ttttaccagc
     9781 tattttaact attacagcaa gtggtaatct taatacacca tctgcatcaa cttcatctat
     9841 atttggtcct attatttcta tcttatggtc ttcaacttct gttgcttctt tcttaacaac
     9901 aagctcccaa gcttcagttc tgtttccacc aaactcagca aacatgtcgc tctttctaat
     9961 tctctcacct tcaaatgctg ctgcaaatga aactgggata ggtatttcag taacttttat
    10021 ttttattcct ctagcttcta atgaagtagc tactatctta tcgtaatctt tttgagttaa
    10081 taggttcatt ggaacttcaa gaactgtttg gtcagtcaca actgggaatc ctaaagctat
    10141 tgctccagca cctgcagata caactaattc acttaatggt ccaaatgcat taacaaatgc
    10201 aggaactcta ttagctgtgt actctaataa tccgtttaaa tcaccaggag tcaatccacc
    10261 gaatattaat gctgctctta ctgcaacaga tacaacgtgt ataacagaag ttacatcata
    10321 tcctagaggt ataactctta gttcaagacc catttttact ccagcttcta tagcttggtc
    10381 tataacttta ccaactaaga aagttagtaa acctttagat tgataatctt ttataacttt
    10441 tgctgctgat tctgcatctg gacactcacc aagaacaact gcaacaccag gtatatctcc
    10501 tgtaactaat ggtacaccaa gtgatctgat tatagggtca gttatatgac ctgcacatgg
    10561 ctcactatat ggagcatcca tagttgaata ttttaatgct tcaattactt ctgcagctaa
    10621 agctgtagct aaaccagcat taaaagcatg ctctagtaaa tgagttctat ttattaaaga
    10681 ttttactact tctaaagcac cttctaagtc ccctaaagtg ttcatctttt gtcctgttgc
    10741 agcatatata caaggtaatg aatatgctgt atcagggaaa gcaactttat gctcttttcc
    10801 attcttttct atagcttcag ctaacatagc ttgagccgca cctaaagctt gttctgaccc
    10861 tgtaaagatt atattatata gattcattct atttccccct ttaatattta cacctatttt
    10921 ttattaacta taccctaaaa accctagaaa gcaaatccac cttcatttaa ctctaagaaa
    10981 gagtctgcat ataaagatgc cccgttgatt atgtcacaag attttacagt tttcattaat
    11041 tctgggtcac atggattcat tatagctgaa ctaaatccat tagccattgc cattgctaag
    11101 aatgcactat ctaaaacagg tctaacatgc ttaggacatc cattagatac gttagataat
    11161 cctcctgtag ttaaaagtcc catctcagtc atcattttta tagcttctaa aacttctact
    11221 tgcttttctt gcataccttt tataactaag cataatgggt caaatagtat atcagttggc
    11281 tccattccta acattaagcc tttttctaat atttcttggc agtaagccat acgctcatca
    11341 ttatctcttg ggataccttc ttttgcacat aaacctataa ccattgcttc atattcagct
    11401 gctatatcta ttaaatcaaa tcttgaacca gcatcagcag agtttattat tggttttgca
    11461 ttagttctat cgtaaacttt aagtccagct tcgatagctt ttttattagc tgtatctaat
    11521 gctataggaa cattgttgaa ctcagattga agtaatttga ttccccatgt cattatttct
    11581 tcgccatctc tttcggcagg tcctatatta aaatctatat agtgtgctcc tgcttctaat
    11641 tgctcttttg ctctttttaa gattggagct gggtctcttt ctgctaatgc ttttcttatt
    11701 gaaggtgata tacaatgtat tctctcacct ataatcataa atttttccat ttataatgtc
    11761 cccctttaaa tttaatatta atagttagtt aatattttat tagtttgttt ggcatgcttt
    11821 ttcttggtat ccttttaaga acttaggtaa ttccatagat tcctctggtc ctataactac
    11881 attccatcct ggtaagttat cttctatatc acctttaagt actgcaactt ttcctggaat
    11941 gataaggtct ttacaattag ttacttcttc taccttactt tctttaatga aagcagagat
    12001 tgagtttcca ccaaatttac cagcagccca agatgtaaga actgaatatc cacctgcatc
    12061 tggtattact aaccatactg gaacttttga tctttctata tcaccagcaa ctatgaagta
    12121 agttaatgca aagtcaacag taactaatac tggagaattt tcatctggat tgttgattgg
    12181 ataaatcttt ggttcaactc tcattggtct ttgtgggtca gtgtatatgt tttgtcttag
    12241 tgcaaataat ggtaatgcct tagcataact aatatcatct attactatta tagaaccgta
    12301 ttttatagtg aatattgatg ataaagcaac ttccatcata gggtttgaat tagataatct
    12361 atttgcaaat actattgaag gatatccaaa tgttctatct tgctctttta aagctattct
    12421 tctaacttgt atagcattag tgtaagtatc ttttatgttt tctcctgtta catctaatac
    12481 taattcttta tatccagcag cttgaattaa ttcaactgtt tcatataatt cttctaagct
    12541 accagctttt acacctaatg gtaagcttgc tcctttaact acttctatca tatctttgta
    12601 gttctcttta gttgctccat ataccattgg tctagcatct tttaacactt caacagcttc
    12661 tttaactact tgtgcatcat cacaagctaa aatataagct acttctaatt cactgccttt
    12721 tattttatct attaatttta aatacgcatc tttatctcca caatactcta aaacagccat
    12781 ttcaactttc atttgttcac caattcttac atagtcaact tttttcatgt tagctatttt
    12841 agaatctact gcttcatcac tcatacaagt acagaatgaa actgcatatc tatttctact
    12901 tactaatgtt ttttcatgtc taaataatac tgtttctcca cctagttcat actcagatgc
    12961 accagcacca acttttaaag ctttcattaa aggtgcagta gcctctgata attttgctat
    13021 agcgtcatca gacatatgtg ggcatttacc tacttctaca gctcctgaag caaccttcat
    13081 agagaaagcc atacaagttg ggaatccaca atccttacag tttttctttg gtgttaattt
    13141 aaatatatct aaagctttta atgccattat tttttccccc taaccttaac aatttaatta
    13201 agctaatgcg ttaaccaatt cttttatagt ttctattgat tttggatgac gaagtataac
    13261 tgcgtttgaa ccacctacta aaacacttgc tgcagttgaa acttccattg ctatagttct
    13321 ttcttctgga caaccccaat ctggctcatc ttctattgga gctatagctt ctttaacatg
    13381 acctacttca aaagctactg gtgttatgat aggcatttgt aatgtcttat cattttgacc
    13441 aaatgctgca agtctaattc tatccatagt agatgcaacg tactcatatc cataaccaac
    13501 tgctgaacat ccaacattca taactatatt ttcaccttta acgcctagtt gagttaacaa
    13561 tacgtttaat tgtttagcta ggtttatatc aacagaagat tcagctccta ctttatgtgc
    13621 ataagccata ccagctgatg ctcctactcc tttgtagtta tcttctactg ctgacataaa
    13681 taagcagtta tgtccatcca atgtttgagc taatttttca aataatttac catctttttc
    13741 atgatttcct gaaccagcta caactaaagg taattttatt gcttcaatta ctgccttagc
    13801 tacatcagca cattcatcaa ctgacttatc taatccattt gggtcagaac catcaaattt
    13861 taaacaaata aaatctgctt gtgtattagc ttcaacatat ttagcccact ctactggaca
    13921 attagctaca tctttgtata actccttata tgaatcagtc cagctttcag gataaacgtc
    13981 tgatatttgt atacctattt ttggagaatt acctacttct ccatcaaaac tataaaaagg
    14041 taatacattt tccccaccta atttaattgc tttttcccct attcctactt caacttctga
    14101 tatttttcca gaatattttt gagtagacat tttaaatgcc atattttttt cccccttaag
    14161 cctattttaa ttctaattta gcaaaaattt ctttcatagc cactttagat tttgaatctt
    14221 caggtaaatt aactaaagga atacctgtag aatcatattc atatatcaat tcatccatag
    14281 gtacaacacc gattagctct aggttatgct tttcaatttc ttctttaaca ccatcattca
    14341 taaccccatt tggaactttg tttacaatta gaagtattct tccaacactt aattttagtt
    14401 cctcagctaa atctcttatt ctagcaacag cttgtatgct acgtcttgag caatcactaa
    14461 ccaataaaag tgtatcaaca tgtctagtga cttttctact tagatgttcc ataccagctt
    14521 cgttgtccat gactaagtat ttgtaatgac cagatatttt gtttacttgt tctctaagta
    14581 tcccatttac aaaacagtaa catccttcac cttctgatct acccattact aataagtcat
    14641 atccttcgcc ttcttcaatt atagaattta atctaaattg aagatattgt gcctttgtca
    14701 taccaccagg gaatgcattg cctaactttt ctctttgatt tacttcttct cttatttctc
    14761 ctattgttgc ttcaacttcg atacctaata cttcatttat gttagcattg gcatcagcat
    14821 ctacaactaa tactggtccc tttttgtcct taaccaaata atcaatcaaa agccctgtga
    14881 gacttgtttt accagttcca ccttttcctg caacagctat attgtatccc attttaagac
    14941 ttccccctta aatctagcca cagagcctta tgtactctgt ctgtctatta taatttaggt
    15001 acagaatgaa cacattctcc atgtacatca tgaagtgctt ccattagccc ttcagataaa
    15061 cttggatgtg gatgtattgc atcccctact tgttctactg ttaatcctag atgaacagca
    15121 agtgacaact ctgtcaataa atctgtagca tgaggtccta ctactgctgc acctattatc
    15181 ttatcagttt ctttgtcagc tataactttt acaaatcctt ggaaatgtcc tatagcttgt
    15241 gcttttccaa gccctctaaa atcaaattga cctacattgt attctacacc ttcagcttca
    15301 agttgctttt cagtcttacc aacaccagca acttcaggct cagtataaac acatcttgga
    15361 attgctctat aatctactac cttagtctta cctaaagcat tttcaactgc tacgatacct
    15421 tctttagaag caacatgtgc taggaaagga gtatctatta tatcacctat tgcgtatata
    15481 ccttctacat tagtttcaag atgttcatta actactactt ttcctctttc catttcaatt
    15541 cctatatctt ctactccaga attgtcaagg ttaggtcttc taccaacaca tactaatgca
    15601 tattgtgctt ctataacctt tccattagaa agagttgcaa cagccttacc atcaactact
    15661 tcacaagttt gtactccaat acctgtgata accttaattt tatctttctt aaattgtcta
    15721 agtagttgtt tagctacatc tttgtcttca tttaatagta tttggtcaac catctcaact
    15781 atagttactt ctgttcctaa ggctctaaag aactgcccta tctcacatcc tataactcca
    15841 ccacctacta ttaacataga ctcaggtatc tcttcaagtc caagaacttc atcactagtt
    15901 attacaactt ttccatcata tgggaacatt cttggtacaa ctggaacaga accatttgct
    15961 agtattatct tatctgcttt tatagtttct actgttccat catctttagt tacttctata
    16021 gtgtttttat caattaattt tccaaaacca ttaactaagt ttactcctct tttttcaaat
    16081 aagaactcta taccacttat taattggttt acaactttat tctttctttc cattatggca
    16141 gtgaagtttg gcttaactgt accatctatc tctataccaa aatcctttgc ttcttttaca
    16201 gtattcaata ctcctgaaga agcaagaagt gctttagttg gtatacatcc tgcatttaag
    16261 caagttcctc caactcttct tttttcaaca actgttacat ctgcaccaag catagaagct
    16321 tttatagctg ctacatatcc tcctggtcca ccacctacta ctactatctt catccttaat
    16381 tcccccaaac ctaatataat ctacaatctt gcaatactat aaacctgcgg catccagtat
    16441 agcaggtata ttctcttctg ccccaattgc cattatatga acaccatcac ataaatcttc
    16501 ttcttttaat tgtttgataa gttcccctgc catttttatt ccttcactta cccagttttc
    16561 ttttccagct gctcttaatc tttcgatttg ttcatctgga acaaatattc ctggtacatt
    16621 tgcagtcata aatttagcca ttcctggtga ttttaaaggt actattcctg ctaatacctt
    16681 gcaatccata tcccttgtta attctctaaa ctttctcatt gtatttatgt cataaagtgc
    16741 ttgtgtttgg aaaaatttag cccctgcttt tatcttcttt tgcattttta ataattgaac
    16801 ttctattgga gaatattctg gagttactga tgctcctaga taaaaatctg gagaaccttt
    16861 taacttatta ccagccatgt ccgtacctgc cattaaagtc tcagcagttt gaagtatccc
    16921 cacagaatct aaatcaaaca ctcccttagc ttgtggatgg tctcctacgc tagtatggtc
    16981 tcctgttaat gccaacagat tgtttatccc aaatacacct gctgacaaca tttctccttg
    17041 tatagctatt ctatttctat ctctccctgt tatttgaata actggctcta atccagcatc
    17101 ttttaataat ttacatgtag caagagaagt tgctctcata actgccgatt gaaagtctgt
    17161 tacatttgct gcatgaactc ttccaactaa tggctttgca cattctatta aatgtgaaag
    17221 gtcagtccct tttggaggag ccatttcagt agtaactgca aattttccac tttctaatgt
    17281 ttctcttaat aagctcatct ctaatccccc ttttaacctt ctaaaaaaaa cttttatgaa
    17341 tttgctgctg cttctttctt tttctttgca cttaaacttc ttggattatt ttgcttagaa
    17401 tagtcttttg gtggtcttat ttctgctaag ttatctaatt gtcctatagc ttctagtctt
    17461 tcatatatct ttatccaagc acaatcattc tctgagttta cttcacactt accattttta
    17521 gctccaccac aagccccatt cataagacct ttagcacaca tagttactgg acatatccca
    17581 cctgtccaac caagttcaca gtctccacaa gccttacagg cttcttcata ttctccaact
    17641 ctttgtactt cccctataaa caaagtattg ttggctggat aaactggctt attttttaga
    17701 ttcttaacta ttgtctgtgt accatctcca catgctaaag acaaaactgc atcagcttcc
    17761 ttagtctcct cttttagagc ctttaaatcc tttttagact ttaaaaggtt acaagcaggg
    17821 tcaagcatta catatcctaa gatttttttg ccttcacctt caagagtttc cttcattttt
    17881 aaaacttctt cttccccacc acttttacaa gttgctgcac attgattaca accaactaaa
    17941 accagcttgt caaaatcctt caagtaccct agcacttctt ctaatggttt attttcagaa
    18001 attatcatat ctacttcccc cctaaacctt ccacatatat atatctattt attgttttgt
    18061 aatttacaag ccttaactac atgttttgca agtatagatg ttgtaactga accaaccccc
    18121 gctggaactg gagttatcat tgatactttg tctaaaactg catttgtatc aacatctcca
    18181 cataaatttc cttcttcgtc aacatttata ccaacgtcta taactaccgc tccatcttta
    18241 acaaaactct catcaaccat cttagctcta ccaatagctg ctattagcac atctgcctct
    18301 gctgctacac ctgatagatt tttagttttt gaatgacata ttgtaacagt tgcatgttca
    18361 cttaataaaa gcattgatac tggctttcca actaccattg atcttccaag aactgttacc
    18421 ttacttcctt ttaaatcaac attatagtgt tttaatatct caactactgc tgtaggtgta
    18481 catggtggaa atccactttt atctccttcc attaccttag cagagtttat tggactaaaa
    18541 caatctacgt ctttttccgg tgcaataaca tattttataa cttcttcatt taagtgtttt
    18601 ggaagaggtc taaaacataa tataccatta acatttttgt catcattaac tctctttaat
    18661 acatcaatat attcctcttg tgttatatcc tcagctaatt ctaacacttc tgttgttata
    18721 cctatgtttt gacatctctt tagtgctcct ctttcataag acaaatcatc acttcttgcc
    18781 ccaactctaa ctattgttag tttaggattt ataccttctt taaccagtaa atcgacttct
    18841 tttattaatt cttcactaat tttatcagct acaggttttc cttttataat ttgtccttta
    18901 gttgacattc cttccataca ttcacaactc ccctacaagc aaacttaaat taatttattc
    18961 tatataccac atattaataa catagtatct actttcgtca tttaaaaatt attttccaag
    19021 tgctttttca actttggcat aaacttcatc acaaattttt acaccatctt caaccaactt
    19081 atctacttct gtttttacct tattaacata ttctttatct tgtatagaat ttatatttat
    19141 aactacattt aattgtccac caagtatagc agctcttaag cactgaactc ctacaccaac
    19201 atcacttata gcaagtcttg aacctttatc aactaaatct tcatgtaact taatagcttc
    19261 atagcaaact cttactatgt ttaatggtac ttcacaagca acttttaatg ccttttccat
    19321 tgtttctgct tttattcttt tttcttcttc tgtttcttta ggaagcccgt aagcttttga
    19381 taatggtaag aaacattctg catcatcatc tatcattttt aataaatctt tttctagctt
    19441 tcctgcctta cttaatatct cttttacact ttcttcatac tcagcatact tcttcttccc
    19501 tattgtaagg ttacatacca tactacctaa agccatacct attgcaccta ctagtgcagc
    19561 agctccccca ccacctggta ctgcttcttt agaagatagt acttctacaa aatcaacaca
    19621 agttttatct gctattttca tgataatcct ccttgaaagg atgtaggaaa atatctcaaa
    19681 ttattcgaga tattttacga actattttaa gatatctcct actctttatt tataaattaa
    19741 tttccttaga ataatccagc tattactcca ttttcatcaa catctatttt ttctgctgct
    19801 ggaacttttg gtaatcctgg catcttcatg atttctccag taagtgcaac tataaatcca
    19861 gctcctgctg aaattgttgc ttgtcttaca gttattctaa atcctgttgg tctacctaat
    19921 ttagtttggt catcagttaa agaatattga gtcttagcca tacatactgg aactttagta
    19981 aatccaagtc tttctaagtt agctatttcc ttatcagctt ctggagtata atctacacca
    20041 tctgctccat aaatcttagt agctatagca tttatcttgt cttttataga taaatcatct
    20101 tcatagcaga atctaaagtt attttcttct tcatccaata atcttaaaac ttctttagct
    20161 actgcaattc caccttctcc accttttgcc catacttcag aaagagcaac attaacacct
    20221 aattccttac acttgtcttc aacaagttta agttcagctt cagtatcaag tgggaatcta
    20281 ttgattgcaa caactgctgg taatccatat acttgagtta tgttttcaac atgttttaat
    20341 aagtttggaa gtcctttttc aagagcttct aggttttcgt tatttaagtc tgctttagca
    20401 actccaccat tgtattttaa tgctcttact gtagcaacta ttataacagc atctggtttt
    20461 aagttagcca ttctacattt tatatctaag aatttttctg ctccaaggtc agcaccgaaa
    20521 ccagcctcag ttattacata atctgcaaag tgcatagcca ttcttgtagc tataacagag
    20581 ttacatccat gagcgatatt tgcgaatggt ccaccgtgta caaatgatgg tgttccttca
    20641 agagtttgaa ctaggtttgg ttttaaagca tcttttagta atgcagccat agcaccatta
    20701 gcttttaatt gtctagctgt tacaggttca ccttcgtagc tgtatccaac tattatgttt
    20761 cctaatcttt cttttaaatc agatatatca cttgccaaac agaacgctgc cattatttca
    20821 gaagcaacag ttatatcgaa tccatcttgt ctaactgctc catcaccttt tttacccata
    20881 ccatcaacga tatttctaag ttgtctatcg ttcatgtcta cacatcttct ccaagttatt
    20941 ttcttagggt ctattctaag tgcatttcct tggtgaatat ggttatcaag catagcagct
    21001 aaaagattgt tagcagctcc tatagcatga aaatctcctg tgaagtgtaa gtttatatct
    21061 tccataggaa caacttgtgc atatcctcca ccagctgctc cacctttaac accaaatact
    21121 ggtcctagag atggttctct taaagcaact aatacatttt tgtctaattt agcaaatgca
    21181 tcagcaacac ctatagtagt agtagttttt ccttcacctg ctggagttgg gtttatagct
    21241 gtagttagta ttaacttagc ttttttacca gtctctcttt ttagtagatt gtagtcaact
    21301 tttgctttgt atttaccata aagttctaca tcatcttcac ctagtcctaa tttttttgca
    21361 acctctctaa tatcttgagg cttagcttct tgagctattt caatgtcaga tttaaatccc
    21421 ataactgaat ctcctcccac aacctaaata gatttaaaat ataatttttg taaaaattgt
    21481 attaatatgt aaaaattata ttttataaga taattataca tgtaaaatta cttaaaaact
    21541 agctactaag ctattaaaat attcaaactt aatagctagt tatttatcta attacttact
    21601 tattacctat ctattgccta tgtctaacct ttaaccaact taaaatccca aatcaaaaat
    21661 tctatctatt ttatttatcc ttaagtgaca ttaatttatc ttttattgct ttaatctgtt
    21721 ctttcgttgt ttcactagaa tcatatggag attgattagc tctatcagaa tcaataacat
    21781 cttggttata ataaataaat cctaaagatt ctccatcttc taagttttgg attataaatt
    21841 cttcatcttc cttatttcta attttatttc ctactacaaa tactttattt acacctatat
    21901 catgtccaag tttcttaact tttctataag tctgtagact tctctctcct ggttctacaa
    21961 ctactataaa agcatctaca ccttgggctg ttcctcttcc taagtgttca attcccgctt
    22021 ccatatccat aaccactaca tctttatttt gtagtattaa atgtgagcag agtctcttca
    22081 aaagaacatg ctctggacat acacatccag taccaccaga atctactgtt cccaatgtaa
    22141 gtaatcttac tccattgtat tctttacaga aattttcagg gatatcgtct actttaggat
    22201 tcatcttaaa catttttcca aatgaaccta cagaagcagc tgttctatct gaaaccaatt
    22261 ttttcatttc tgatataggt actattgatt catatacttc ttttggaaaa cctagtgcta
    22321 aagctaaatt agcatcaggg tcggcatcaa cagcaacaac tctgtatccg tcttcagcaa
    22381 acatcctaga gagcattgaa gaaaaagtcg ttttacctac tccaccttta cctgttattg
    22441 ctatcttcat aatccctacc accttttatt actatttgct ttttagtagt taattttaca
    22501 gttaaatatt ttttatactc tccattttta tgcactaaaa ataaactaca gctagtcctt
    22561 acaggaacca attagattta ccctactagc ttttcaaagc agcatgtata gttttattat
    22621 tctttaatat tgtttatatt ttatttattc agtatattca ttatattttg atgttcttta
    22681 ctttaatatt tgactttatt caaccagaaa cttcttataa aaacgtttat tctataactt
    22741 cgctattttt taagcttcag ccttttctgc cattttttct tcaactaatg cttcaaagtg
    22801 cacacgtttc ttttctatat cttctattat tgtagctgct aattcttttg gatttgtatt
    22861 tacagtaaat gtagctccaa ctttatcttt taattcatta gttaatatat ccactgctct
    22921 tgaagaacca gctattggag gcattattcc taaataagtg tctataccag aagctacaac
    22981 atagcatcct atagcaactg ctttttctga catccactct ggagctatac ctacaactgg
    23041 aaggtcacac atatccatat ctaagtaatt agctgctgct ccaactaaat ctagtatacg
    23101 gcttatatca acacaagaac ccatatgtag tactggtggg attcctacta atttacagac
    23161 agttgcaaga cctttacctg catatttttc agccgcatca gattccatta aaccaaactt
    23221 agctgctgct gaagctccac aacctgtagt aactactatt atgtcgtttt tgattaattc
    23281 tttcattatt gtcacgtgag cctcatttga aactccttta gcattgttac atccaactac
    23341 accagctgct cctcttaata ctccagcttt tagacagtct gttaatggct taactgttcc
    23401 tgctgggtct atatgagagt ttacaactct gtctaattga tttactactg cttctacact
    23461 atacccaact gttgcccctg attttaattc aggtataagt acttttgatt tatctctatt
    23521 tttaaagttt aaaatagctt ctttaactat tgctttagca tcatcaagtg cttgttcttc
    23581 tctaaattca atataagttg aacctgttat cttagctttt ggagaagttg ttacaaattt
    23641 agtatggtaa caatctgcaa ctctagctaa tgcagggaat atacattgaa cgtcaactat
    23701 tacagcttca actgctccag ttattattgc aagttcttgt tgatggaagt ctcctgctat
    23761 ttttacccca tgtctcattg taatctcatt acctgtacaa cacatacctg ctaagtttat
    23821 tccatcagca ccaacttctt tagctaaagc tactaagtct ggctcttctg atgctaaaac
    23881 tatcatttct gataatgctg gctcatgtcc atgtaatatt atgttaactt tattttcttc
    23941 taatactgct aaattagcct ctgttcttct tggcactgga gttccaaata agatatcgct
    24001 tagacgagta cctatcatag aaccacccca accatcagcc atagaagttc taagactcat
    24061 gtgtattaaa ctatctatat caccagtaca tcctatatga gtagagtgca ttattgttgc
    24121 aatttctcta tctatagctc ttggttctat agcatattct ttccatattt tttgtcttgg
    24181 ttctggtgca ttttttataa atcttaatgt cccaaaaggt tttccaaatt ccattaatgc
    24241 aatttctgcc acttcatgtg ctacatcata gatatctcta ccttctgtct ctacatccca
    24301 ttctttagct aaagtaatta acttagcttc gtctcttatt ttatagtttc catttggatc
    24361 agctaatgct aatgtatgtg ctatatctct agcatggtct gaatgggcag ctgtcccacc
    24421 agcaaccatt cttgcaaagt ttctacctac tatagtatgc tcatctgctc cacataaccc
    24481 ccttggagtc ttagggctta ttctacatgg tcccattcca catattctac agcaaacacc
    24541 ttgaagacca aaaccacatt gtgccttaaa gccttctttt ctatcataca ttgtttctac
    24601 gccttcttct ctagctttag ctatcatctt ctggctgttt aaatcaattg ttaacatttt
    24661 ttcatccata ttcctatccc ctttttttga tttttaaaat cccccaggtg actgacttca
    24721 tgtcataaaa taataaaata aatgctttct aagttgatta tatactaatt ttcagtgctt
    24781 tgctttagat ttaaatattt ttttaacaaa attagataat acataaaaat tttattttct
    24841 acacttaatt taagttgatt ttatcgcttg tttttggcta tttgttaata tctaaaccta
    24901 cttttttgat acttcagttt aattaaatta tgttttgatt tttttaaatc aaatttgaaa
    24961 tttttctagt catttttata ttattgcaga ttgtttgtgt atttattatc tttcactact
    25021 gtttggactt attacagatt tttactccct aaacttaatt agtaaaaact tcttagaata
    25081 gcattttttg gactaatttt tttgaatttt tttatttttc ctactttaat attaattata
    25141 gcttcaaatt gaacatttgt atatattcat ttgaagctat aattattttt tatataaaac
    25201 ttttttaata cagcattaat ttagacattt tgactatttt ttctacttta attcatttat
    25261 ttcaaacgat ttatcctatt tcatattttt agccacaata gcaccatatg caatagaata
    25321 taatttagat tcatgtgaat cagctcttga cactaaaact ataggggctt ttgcacccat
    25381 aattatacct gctgatttag agtttgccat atatgtaaag gctttcccta ttccattacc
    25441 aacctctatt gttggaacca atatgatatc tacatctcca gatacttcac ttttaaaccc
    25501 ttttatctca ctagcttctt ttgaaacagc caaatcaaaa gccagaggac cttctacaat
    25561 tacattttca ccaaattctc cttctttaca agcttttgct aacatatctg catctacagt
    25621 tgcttgcatc ttaggattta ctttctcttt agctgccaag catgcaactt ttacagtttc
    25681 cattcctaaa gcttttgcag cttttataga attttttaat atctttgcct tttgttcata
    25741 atcaggagct atattcatac ctccatctgt agttataagt aatttatggt atttttctaa
    25801 ctcatatacc ataacatgac ttaataaact atcagttcta agaccatagt ctttatttaa
    25861 cacttctttt aatatgacag atgtatctaa taaccctttc attacaaagt ctgcttcttt
    25921 acttgatact aatttaactg ctatttctgc acactctttg atatcatcct tatccactat
    25981 tttgatacca cttaaatcaa atcctatttc ttttgatatt tctcttattt tgccttcatt
    26041 tccaaccaat ataggagtaa ttatatttct ttctacagca tcctttattg ccattaaaac
    26101 ttctttatca tgagctgctg ctacagagag tacaattttc tctcctcccc taagttggtc
    26161 taaaatatca tctaattttt ttatcaaaag taacccctcc cttatatata tttaactaaa
    26221 taataccata tttgctatac cttttctcaa tttatttttt atgttataaa gaatttatat
    26281 aactaaaaga tagtttaata ttttattccg ggatttcatc aataactctt aaaagctttg
    26341 caggaacacc acctacaata gtattttctg caacattttt tgttacaact gctccagcag
    26401 cgataattgc attatctcca atagtaactc ctggaactac agttacatta gcaccaatcc
    26461 atacattttt tcctattgtg attggagaag gataagttgt accacgtttc tctggtgcaa
    26521 caccatggtt aagtgtagcc aataccacat tatgcccaat ttgcgtgtta tccccaatct
    26581 caatcccccc ctggtcttga aaatggcaac tgctatttat aaaaacattt tttcctattg
    26641 tgatgttttt tccacaatct gtataaaatg gtggaaacat aaaaaatgat tcatctactt
    26701 tttttccaat taactcagaa aataaactac ggacttcttc tggctcatga taagaagaat
    26761 ttaacttact tgtgattttc attgcttcat tactcaacct aaccataaat tcatgtattt
    26821 ctgatttact aaaaacaggt tttctttgat tgacatgtgt taaaaattca ttgatattca
    26881 ttttatgcat cctttcaata tacgctttaa tttatttgta cattctaaat gatattttaa
    26941 taaaaattaa tttaatttat ttttattaaa atatcattgc tccttttagt gtaatataca
    27001 attatatcta tatcaaatat ctatattaaa tacttaacta tacttaaaaa gcatataaat
    27061 tgtataatta tataataaat aagttaatat tgaggaggga taattatgga aataagagta
    27121 ttgagatact ttttagctat tgcaagagaa gaaagcatat ctggtgcagc aaccttttta
    27181 catataacac aaccaacact ttctagacaa attatggaac ttgaagaaga gttgggagtt
    27241 aaattattgg ttcgaggaaa tagaaaaatc accttaacaa atgaaggtag acttttacat
    27301 aaaagagcag aggaaattat atcattagtt gataaggttg aattagaatt tgacaatccc
    27361 aatgagatta ttagtggtga tatatatata ggaagtggtg aaactgaagc tatgcgactt
    27421 atagctaaaa ctgcccatca ttttcatcaa aaatacccca atgtaagata ccaccttttt
    27481 agtgggaatg ctgatgatgt gactgaaaaa ttagacaagg gactgcttga ttttggtatt
    27541 ttaattgaac ctgcaaatat acagaagtat gattatacac aaattcctgc tactgatgta
    27601 tggggtttac taatgcgtaa agatagtgcc cttgcatcac aaaacacaat aaatccaaaa
    27661 gatttatgca atatacctct tattatttca agacaaacac ttgttgataa agcaatttct
    27721 aaatggcttc aaggcagtaa tgaaaaacta agcattgttg caacatacaa tttagtttat
    27781 aatgcttctc ttttggtaga agaaggtctg ggctatgctc tgtgtttaga tagactcatc
    27841 aatactacag aaaatagtaa tctttgtttt agacctctat ctcctccttt tgaagctaaa
    27901 ttaaatatta tatggaaaaa acatcaaata ttttcaaagg cagcagaaaa atttttactt
    27961 cagatacaaa aagatttttc ctctatcatg ttggatacat ctaagttgga tacacctaaa
    28021 gaaaaataaa taataataaa aagtgagagt gtctcaaaat ggacttttta cttcatgagg
    28081 cactcttttt tatatgaagt aaaatatata ttcatcattt cagcactaaa aaagaggctt
    28141 atctctattt tgagatagcc ccacttaacc aataaaatta aatacaacct aacaatatcc
    28201 ttacttattt attccaagtt ctcttaaaca agcaatcgta atttcatctt gtggatttaa
    28261 ttcataagct ctttctatat agtaagttgc atcatcaata tcaccattat ttaaataggc
    28321 cattcctaaa ttgcaaagta tttctgggtc ttcctttatc ttagccgctt gttcaaaata
    28381 ctctattgct ttatctaaat tattcttata agcctcacaa agccccaatt ccactatagt
    28441 atccacctga ttaggcttta ttatcaatat cttctcaaga tacatcttag cctgctctat
    28501 ttcgcccata cccttatatc caagtgctat cataaacagc aagttccacc aatccatatg
    28561 ttcttcttct aatggaagta acttctctaa tccttcttta aacttacctt ggaatacaag
    28621 tgtataccct tcttcatatt gaactttaaa gtccatcttg cccagattct cttgtacctc
    28681 agctactaaa tcagcatcta accctagcct taacgcctct tcccaagtca acttagtctt
    28741 taaatattga ccttgattat aataatggta gcctaagtga taataaccta atgcaaaatt
    28801 ctcatcaaca tcaattacct tttcaagctt agccatggct tcaagtaaga atgcattcat
    28861 tgccttatca tccatatctt tttgatactg tttagctatc tcttggcaaa caatcgcata
    28921 gttatacatt gcatctaaat catctggata cattgttata aatgctttta tatatattaa
    28981 agaatctgta tgttcaccaa tctcaaagta ttttctcgac atatatccca tatacgattt
    29041 caaatccaaa tcaatatctt ttgataaagc atttaaaaac ttttcatact ctgcattgtt
    29101 tttaaactta ctatctattc caattatata tatcattgca tctgctatag acattgaatt
    29161 taacttatct tgagcagtct tttcttttat gccttttact aatacttcat tttttatagg
    29221 tacatctaat ccaccttttg gtacttcata gctttttaat ttaaactctc caccatgttt
    29281 tatagttata aaagctaatt cctctgcttt ttttagcaaa tatttttcta tcttaaaatc
    29341 cattttatcc tcctaaatac ttaaatctct actttatgag ttttagattt ctcaaatatt
    29401 tttttatcat catactcaaa taagactaca ttttctactt ctcttatttc tgtatttata
    29461 tcatttttta aatccacaac attaaatcta aatggctcaa aatgaacata ttttattata
    29521 tgtttagctg gtttatcaac aaaactaggt aaatatgttg ataagttatc attatcttgt
    29581 aaaaatacat ggcatctatt cttaaaatca tccatatcta acttattaaa aaactgtggc
    29641 atatttgatg ttttacccag tgatatatag tcatatttta atatatcatt gaatacatca
    29701 ttatctatgt tgattttttc attatagaaa tccatcaata ttttatataa ctgattcttt
    29761 ccttgtgcca aatcaaagta gccattttca tcaaaatact tggcaaaaac ctcaaagaat
    29821 ttaaaagggc tttgtttata aaatctgcct ataatatatc tcatagataa cacaaagttt
    29881 ttagaattgt agtatctctc cagtatatct tctatatcct ttagcttcaa agtttcttca
    29941 tagcttatat aattattgta taaaaactca tatggagcat aatccttata tttaaaacca
    30001 tgctcatctg caacttttct catacctgtc cctttaatca tctttaaaaa gcctaattgc
    30061 agatgctcta tacctagatt aaatacatca ttaaaagaat tttcaaagct cttataatct
    30121 tcataaggca gtcctgcaat taagtctaag tgctgatgta tatttctata agatgctaca
    30181 gtttgtacaa catgagacaa ctttttaaaa tcatcccttc tacctactgc atcaagtgtt
    30241 ttttcatttg ttgactgaac acctatttca aactggaaaa gtccttcttt acaatcagcc
    30301 aaaaactcaa gcatcttatc atctatcaaa tatgccgtaa cttcaaaatg ataagttgta
    30361 tatccattat cgtttttcat aagaaagtcc atgatttcca tagccacttt tctatttgca
    30421 ttgaatgtcc tatcaataaa ctttatctgt gatactcttg catctattaa agcttttaaa
    30481 tcactcttta tcctgtctaa gctaaaatac cttagacctg gaatagtaga tgataaacag
    30541 tactggcagt taaaaggaca acctcttgat gattcataat acactattct attttcatat
    30601 tcttttggat tcaagttttc ataaggacta ggaatctcat ccaaattttg aagtaattcc
    30661 ataggcttat tcactattat ctcattgcct tgtctatata caagtccatt tacatcattt
    30721 atttccatct ttccttgcaa gtgaagtact aagtctctaa aaattaattc tccctctcca
    30781 taaagtatat aatccacaaa atcatacttt ttcattgcct cataactatc ataactcact
    30841 tccggtccgc ccaaagctat tttagtatta ggtctaattt tttttagatt atcacaaagc
    30901 ttaactatat ctcccacatt ccagatatat gttgagaaca atattatatc atattcgttt
    30961 ttatatatat ccttcaaaat atagtccaaa tcgttgttta tcgtgtattc tttcatacta
    31021 acttctatca aatctcttac aaattctttt aaatatctta tagccaaatt tgtatgaata
    31081 aacttagaat ttaatgttgt taataaaatt ttcaaatatt tctcacctct ttttaagatt
    31141 atactgtctt tttgtacccc ttataggtta aaatagtata tgtaaagtct aaataatatc
    31201 tatataatta atttattaat ttcaagtaat gtgaggaaag gaagtttttt atggaattaa
    31261 ttgaaaaagt atctatcaac tctttatcaa aaagagattt actacttatt ataaagtctc
    31321 ttgaatacac taatgaaaat actcaaatta gtgatttcat tgaccttagg aataatatag
    31381 taaaggaatt atgtttttta actgacacta aggaaaaaga ttttttagat tatctagaaa
    31441 aaaatagcac actctaataa cctaaacctt tttaaacttt aatatataaa tacctgattt
    31501 agaatttatt tttatgctta atatattatc attaaaggtt ttcgacataa ctgttatttg
    31561 attaaatata gggtctatta tagtattacc ttcttcaagt cttataagac ttcttgggtc
    31621 tatgctttta ttatatgcct taactccaat tttatatttt ctcaatttaa caacatattc
    31681 ggacatagaa aaaataaaat ctatattatc ttcatttaat ttactatctt cacccaaaat
    31741 ggataatata gattttttta tgctattaat tccttcattt tctacagttc tatcttccat
    31801 atatacatca tatagaacat ttaaaacatt taataaaata ctttcttcat cataatcttc
    31861 agatttcaaa tcaatgtact tatcaattaa ttgtattgct ttaatccttg ccatctgaaa
    31921 ccttacaaca tctgatttgt ctaattctat agaaaatcct attatcttgt ctttaatact
    31981 ctgtagtaat tcgacatatt ctattttact attatttata agattgtgca taatggaatt
    32041 atacactaca gaacctaata aataatcaaa taaaagtttt tcttttttaa aataaataac
    32101 atacttt
//
LOCUS       DAEMAE010000015        30644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEMAE010000015 DAEMAE010000000
VERSION     DAEMAE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.015"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            126..998
                     /locus_tag="KPH93_000632"
     CDS             126..998
                     /locus_tag="KPH93_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1687469.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGNYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENVEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            998..1663
                     /locus_tag="KPH93_000633"
     CDS             998..1663
                     /locus_tag="KPH93_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG1687470.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKNHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            1684..2541
                     /locus_tag="KPH93_000634"
     CDS             1684..2541
                     /locus_tag="KPH93_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1687471.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIGLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPQRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNVSVFYGGVSGLVSIDKFLKEDKVTYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(2697..5243)
                     /locus_tag="KPH93_000635"
     CDS             complement(2697..5243)
                     /locus_tag="KPH93_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1687472.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQKVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSAPYTAGSEVFVPQDKKSYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTGDHAYIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIVVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTASGV
                     YEGLYQKLMTYSCIAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPTEEYTGQNAKLIAVSMVTKDNNIIDIFTSSSMNVSLTPETNGKLDTPERQNVNIS
                     FVKVVPPDSPPGIRSSDMKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(5243..5929)
                     /locus_tag="KPH93_000636"
     CDS             complement(5243..5929)
                     /locus_tag="KPH93_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687473.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(6012..7001)
                     /locus_tag="KPH93_000637"
     CDS             complement(6012..7001)
                     /locus_tag="KPH93_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1687474.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISTMTGILSIVSATTFG
                     TAFFVFTKNRYKSIAQISEQINLVLHNANHLYITESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     KILGDLNWLSEAIQNIFKNCMESVGDNGKIHIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(6988..7674)
                     /locus_tag="KPH93_000638"
     CDS             complement(6988..7674)
                     /locus_tag="KPH93_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687475.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEEQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(7671..7808)
                     /locus_tag="KPH93_000639"
     CDS             complement(7671..7808)
                     /locus_tag="KPH93_000639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687476.1"
                     /translation="MEVLCIDYIRTEETNENCVIISKIVVYYCSAGIYKTNKIKEDES
                     K"
     gene            8084..8413
                     /locus_tag="KPH93_000640"
     CDS             8084..8413
                     /locus_tag="KPH93_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1687477.1"
                     /translation="MDLNKEVLKGHIDTLILSILNKGDSYGYEIAKTVRDETTFELKE
                     STLYVSLKRLESKGLIKSYWGSDQGLGSRRKYYNITDEGKENLELKIQEWNFIQSVMN
                     KFLKRGE"
     gene            8418..8867
                     /locus_tag="KPH93_000641"
     CDS             8418..8867
                     /locus_tag="KPH93_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687478.1"
                     /translation="MKIIEEYLEALYKNDTSKEIQDLKEELKEHLIDSTNEFIENGYD
                     INEAQNKAIERFDGSNGVSVELRSIFKQKIEPKKVLLRKLSNSRIVFLNLFGWLLAAT
                     AYTINRGFGHIAPPWLIILLIISIVILIILTIIIKRLKKEIKNDYNI"
     gene            8934..9311
                     /locus_tag="KPH93_000642"
     CDS             8934..9311
                     /locus_tag="KPH93_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG1687479.1"
                     /translation="MVKKILLEKFLDTSGVSYINEKLNLFKDTKWISFGTYIMTVDSF
                     CYREDSKHYNPIMSTFDYESEIFIVNALYKENIYSIKSKKLYRNVSKGDKIIVTIKYS
                     YDKYENLIHEEIHDYHLYPLSLD"
     gene            complement(9494..12700)
                     /gene="carB"
                     /locus_tag="KPH93_000643"
     CDS             complement(9494..12700)
                     /gene="carB"
                     /locus_tag="KPH93_000643"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG1687480.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLEKLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFARD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLSAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEAETVNRVEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(12880..13929)
                     /gene="carA"
                     /locus_tag="KPH93_000644"
     CDS             complement(12880..13929)
                     /gene="carA"
                     /locus_tag="KPH93_000644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG1687481.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(14186..17392)
                     /gene="carB"
                     /locus_tag="KPH93_000645"
     CDS             complement(14186..17392)
                     /gene="carB"
                     /locus_tag="KPH93_000645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG1687482.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVIGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(17445..18488)
                     /gene="carA"
                     /locus_tag="KPH93_000646"
     CDS             complement(17445..18488)
                     /gene="carA"
                     /locus_tag="KPH93_000646"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG1687483.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKETIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEITHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(18514..19230)
                     /gene="pyrF"
                     /locus_tag="KPH93_000647"
     CDS             complement(18514..19230)
                     /gene="pyrF"
                     /locus_tag="KPH93_000647"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG1687484.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(19606..20238)
                     /locus_tag="KPH93_000648"
     CDS             complement(19606..20238)
                     /locus_tag="KPH93_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG1687485.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLDASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKEE"
     gene            complement(20262..21176)
                     /locus_tag="KPH93_000649"
     CDS             complement(20262..21176)
                     /locus_tag="KPH93_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687486.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEDLFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEEIQEENVILEKEESQDE"
     gene            21776..23005
                     /locus_tag="KPH93_000650"
     CDS             21776..23005
                     /locus_tag="KPH93_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG1687487.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(23210..25591)
                     /locus_tag="KPH93_000651"
     CDS             complement(23210..25591)
                     /locus_tag="KPH93_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG1687488.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDECVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNITLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(25563..26288)
                     /locus_tag="KPH93_000652"
     CDS             complement(25563..26288)
                     /locus_tag="KPH93_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG1687489.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDIYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(26278..26733)
                     /locus_tag="KPH93_000653"
     CDS             complement(26278..26733)
                     /locus_tag="KPH93_000653"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG1687490.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAVECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(27015..28196)
                     /locus_tag="KPH93_000654"
     CDS             complement(27015..28196)
                     /locus_tag="KPH93_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1687491.1"
                     /translation="MKNKSLLNHMFRPILDLIISCGLTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHNGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(28186..28878)
                     /locus_tag="KPH93_000655"
     CDS             complement(28186..28878)
                     /locus_tag="KPH93_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687492.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKN
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFNGSNNEDKDVIEVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(29012..29728)
                     /locus_tag="KPH93_000656"
     CDS             complement(29012..29728)
                     /locus_tag="KPH93_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1687493.1"
                     /translation="MKKFKLIGAIILVVFISVFISKERLLKKDKDPIVVAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            29854..30588
                     /locus_tag="KPH93_000657"
     CDS             29854..30588
                     /locus_tag="KPH93_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBG1687494.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     NYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT         9822 a         5483 c         3761 g        11578 t
ORIGIN      
        1 tatgcttaaa acagtaataa tgttaatagt aaatgtaaag aatagaaagt atctaaaaca
       61 gcaaaaaatg actaattttg tggtataata cataggattg agagttattt tataggagga
      121 aaaatatgtt attattagca gataaatgga aagattatga acttatagat atgggaaatg
      181 gagaaaaatt agaacgttgg ggaaattatg tacttagaag accagaccca caggttgtat
      241 ggccaatgga atctgaatgg gctttatgga aaaaccctca tgggcattat cacagaagca
      301 ataaaggtgg aggtcaatgg gaacacaaaa aaagatatcc ggaacaatgg actataaatt
      361 ataaaaactt aaagtttcac attaaaccaa ctggatttaa acatactgga ttattcccag
      421 agcaagcagc taactgggat tggatgatag agaaaataaa gaaagctaac agacctataa
      481 aagtgttgaa tctttttgca tatacaggtg gagctactgt agcatgtgca tctgctggtg
      541 cagaagtatg ccatgttgat gctgcaaaag gtatggttaa ttgggcaaaa gaaaatgtag
      601 aattatcagg tcttaaaaat caaacagtaa gatttatagt ggatgatgtt gtaaagtttg
      661 ttgaaagaga aataagaaga ggtaaaaaat atgatgcaat aataatggac cctccttctt
      721 atggaagagg tcctaaagga gaagtatggc aaatagaaga aaagttatac agttttgtgg
      781 atttatgtat gggagtttta tcagataacc ctttattttt cttgattaat tcttatacaa
      841 caggattttc tccaatagtt cttgaaaatg tattgtctac aacaataggt aaaaagacaa
      901 atacaggaga tatatacggt ggcgaattag gaataccagc ttccagagat ggtaagatac
      961 ttccttgtgg tatatttgga agatgggaga aaaaataatg ataaaagtca tatatgaaga
     1021 taaccattta ttagttgtag aaaaaccagt taacatatta tctcaaggag ataatactaa
     1081 tgataaagat atggtaaatc ttttaaaaga ctatattaaa aaaaagtata acaaacctgg
     1141 aaatgtatat attggtttaa tacatagatt ggatagacca gtaggtggag ttatggtttt
     1201 tgcaaaaaca tcaaaagcag catctagatt gtctgaacag gtaagaagta agactttcaa
     1261 aaaaacttat ctagctgttg ttcatggcaa gataaaatct aactcagata tactaaagga
     1321 ttttttatat aaaaataaaa aaactaatat ggtaactgta gtgaaaaaaa accataaaga
     1381 tgccaaagaa gctgagctta actataatgt attagatttt aaagaaaatt taagtttagt
     1441 tcaagttaat ttaaagactg gtcgttctca tcaaataaga gttcaatttg caagcagaaa
     1501 acatccatta tacggagacc aaagatatgg caaagaaata aataagattg gacaacaaat
     1561 atctctttgg tccaataaga tagaaatttg ccatcctact acaaaagaaa aaatggaatt
     1621 tacatgtcat ccacctgaca aatatccatg gaatatattt taaatctcaa atgaggtgat
     1681 tatatgagta aaaaattttc aataggagaa atgtctaaac ttcataaaat aagcatacag
     1741 acactaagat actatgacca aataggttta tttaaacctg ttcatgttga ccaagaaagc
     1801 aattacagat attactcaat tgaccaattt tcacaattag atattataaa atatttaaaa
     1861 tatttaggta tgcctttaaa agaaattaag tcaaagcttg atgaaaataa tgaagaaatt
     1921 ctaaagttac taacaaataa aattaatata gtagatgaaa aaattaaaga attagagctt
     1981 ataaaaaagg tactaagcac aaagaaagaa actatggaaa aaggtatgaa agtagatgaa
     2041 gttggtaaaa tagtgagaaa acatattcca caaagaagta tcttatctat agaatatgaa
     2101 gaaggattaa gtagtgaaga aactatggaa ctttccagaa gacaaatagc aaatatatta
     2161 gaagataatg tatctgtgtt ctatggtgga gtaagtgggc ttgtatctat agataaattt
     2221 ttaaaagaag ataaagttac ttatgacaat agttttgtta ctgttgaaag agacttattt
     2281 gatataaaat cacaaaaaca tataacagaa atttcatctg gtgattttat ttgtatgaca
     2341 tataaagggc cttacaaaga aaattatacc gcttttaaga agctggttga ttatgttgaa
     2401 tataaaagaa tacctattga aaattatata tatgaaatac ctataataga ccctctatct
     2461 acaaacaacg aagaggaact tctaacagaa attcaaatac aagttaaaaa gagagaaatt
     2521 gacttaagat gtctagtgta gtaatctagt ttacttacaa atcttaatat agttgtctat
     2581 tatcaacata tgatttttct agatagtgat tcctaacagt gagattttca aaaaatagga
     2641 gggaacatgc gtaatgctaa ttccctccta tttttaatta caccatgtca ttttaattaa
     2701 gccatgtcat cacgaagtgc atcaataata ttttcttttt ttatcttatt aatagtatac
     2761 aacattgtaa taaatactat aaataaaaca ctaaatatac taattcctat actaaaccat
     2821 ggaatcacaa aatcgatatc atctgtaacc attactttat gaattagcca tgaagaaatt
     2881 actgctatag gaagtccaaa gaacaatgct ctcataccat aaaaagcaca ctcaaaattc
     2941 atcatctttt ggaaatcatg ctcagacatt cctacagaac gaagcatggc aagctctcta
     3001 cgacgtaact taatattagt tgaaattgta ttaaacacat tggcaatcga aatcagagaa
     3061 atcataatta tgaaagtata agcaaaaaca ttggcaataa aaatgtaatt acggttctca
     3121 tccatcattc catacatatt gtacagattg tactcattcg taactccctc actctcaata
     3181 atttgctcca tttctgcaac tgactgagta ggattttttg attgaaagct taaactttca
     3241 attcctgtag aaacaccagg aatctcaaac ttatctttag ccgaataagg agctaatact
     3301 ctgaagcttg ctttcttcat atctgagctt ctgattcctg gaggagaatc aggtggcacc
     3361 actttgacaa atgaaatatt tacattttgt ctctcaggag tgtccaactt gccattagtt
     3421 tcaggagtga ggctaacatt catagaagaa ctggtaaaaa tatcaataat attattatcc
     3481 tttgttacca tagaaacagc aatcagtttt gcattttgtc cagtatattc ttctgttggc
     3541 aatccaagac ttttaataat attcagataa gttctatcat caagaaattg catatctact
     3601 ggtaattcaa gcatttcact catagaatgt gaaccctcat agttcaaata atcgtctgta
     3661 agctcactta ctttggcaat acatgaatag gtcataagtt tctgatacaa accttcgtaa
     3721 acaccacttg cagtttttag cttatcataa agtttcaaca tttcgttgtc tttcatacct
     3781 tgtgtagaaa aaataatatc ctggtcagta acctctgacc tctgatttga catctgtttt
     3841 aggtctgcta ctaaagcact tgtagatata aatagaacta tgcttaaaac aagcgacaat
     3901 acaatacttc gataacgttt cttgtttctt ttaaagtttt tcatagcaag aactccctca
     3961 agaccataaa ctcgctgtgt tagttttgat gtcttcacag atttagattc aatttttacc
     4021 tcatttgtct gacgaataca ctccatcact ggtgtgttgg cagcctttct tgctggtatg
     4081 taagcagaaa ttaagattgt aaccatactt accactgctg caacgacgat tgcagggata
     4141 gacagtgtta atgtcaacgg aacgtttaca tagagaatat ttgcaaagtt tttagctatg
     4201 acagaaatca caagtccaat actgactata ccgattgcaa caccaatcgg tataccaatc
     4261 gcaccaatgc aaattccttc aaatagcacc gaatttcgta actgttttgc tgtagctcca
     4321 acagatgaaa gtattccaaa ttgatgtgtt cgctcgttca atgacatgtt aaacgaatta
     4381 tatattagaa aaattgagcc tagcatgatt atggcaagca caatgccacc aaccgagtac
     4441 aaaaacatat tgaacaaatt gttatcagaa acacctatga agcgcaacac attgtcattg
     4501 aaaatgtaag catggtctcc agtgctactt gtgtaagagc gaatctgatg tggatttttc
     4561 aatgtgacaa aaaggctaag actgtcagtc ttgacttgtt tatcttcttt ggttatcaag
     4621 gtatatcctg gtgcagaaga ttcttcaaat acaggctttc tacagatacc cacaactgta
     4681 taagatttct tatcctgtgg cacaaaaact tcactcccag cagtgtatgg ggcactctgc
     4741 ccaagctttt cattttcact cattcgtttc cctacatcca aagaaatagt atcacctact
     4801 gcaaacttga caccaccatc tgtcgcaact ttacttgaaa caagaatctc tctactattt
     4861 tcaggtaatc ttccagaaat taaagttata ggcaaagtat caaaagcttc tttgctaaat
     4921 ccagcaataa aaagatatgg tttatctgga tttctgttac cttcaagtgt tgcatagcca
     4981 atattttcaa atgttgcagt atttgcaact tcatcattga ctttctgttc ctgtacaaaa
     5041 gaagaatcaa catccaaaaa ctggacatgc caatcaccat attttataat ggctccattt
     5101 gtcatatagt tcatcaaaga aactccaaag gtagtaactg ctgtaatcat agcagctgaa
     5161 agaacaactc ctataattgt tacaagagtt cgagtgcgat tctttttcaa tccttgcaga
     5221 gcaactttgt taaaaatgtt cattaacggt tcaccttcct ctcatcacgc actacttttc
     5281 catctgaaat acagataatt ctatctgcct gtaaagcaat gttttcatca tgtgtgacaa
     5341 taatgagagt ttggtgatat ttactatgac tctctttcag taactggata atctcttgtc
     5401 catttttact gtctaagcta cctgtaggct cgtcagcaag cataactgct ggtgcattca
     5461 tcaatgcacg accaatggca acacgttgct gttgaccccc agaaagttga tttggtaaat
     5521 gtgtttttcg ttcttttaaa ccaagtaatt ctaacaagtc atttaaccgt tcttggttga
     5581 ccttacgctt atccataagt ataggcaatg tgatattttc cactacattt aatgtgggta
     5641 tcagattgtg aaactgataa atcaatccaa cttgtcgcct acgaaaaata gcaagttttt
     5701 cattgctttg cccatataca tcttgcccct ccagatatat ctttccactt gttggtacgt
     5761 ctactcctgc aatggaatgt agtagtgtag atttaccaga accagaagaa ccaatgattg
     5821 cagtaaactc tcctttttca atagtaagtg agacatggtc aagtgcagta accttatttt
     5881 cattcttacc ataaacctta cataaatctt caatctttaa aaacgccatt atataagtct
     5941 cctttcatat tttataaata tatcatagga ctttcaactt acatttaagt gacatttaaa
     6001 tgagagattc gtcactttgg aaaacgaata acaaatattg caccaccttg tgggtgattt
     6061 tgtgcagtaa ttgttcctcc ttgtctcata ataatcgtct tacaaagtgc caatcctatt
     6121 ccataaccag ctgtacttga atttttccca cgataaaatc tgttaaatag acatggtaaa
     6181 tcttcttttt taaagcctgc tccgttgtca tgaatagtaa gttgagtaaa caaaaagttg
     6241 tcctcacaaa cgatatgaat ttttccatta tcacccacac tttccatgca gtttttaaat
     6301 atattttgga ttgcttctga aagccaattt aaatcgccca aaatctttac tccttttggt
     6361 atatcaatat gtaaagtgat attgtgcagt tccatgggaa tcaaaagttg atgaagtgca
     6421 gaactaatca aattattcac atcaatttgt tctttttgaa agactacaat acctgcatct
     6481 aaacgtgata attttaaaag ggaagtaatc agccaatcca ttcgtataag caattcctct
     6541 gtttctcgta tcagctcttt tctctcatct tcatcagaag tattctctaa cagtgacaaa
     6601 ataagagtta ctgatgtaag aggagtgcga agctggtggg ctatgtcagc tagtgaatca
     6661 gcaaggtgtt ctttttcttt tttcagtgcg taattttgct ctctaatacg taacgtcatt
     6721 ttagttattt cactttgcag aatagaaagt tcaccctcat ctgattcagt aatatacaga
     6781 tggttagcat tatgaagcac aagattaatt tgttctgaaa tctgtgcaat acttttgtat
     6841 cgatttttag taaacacaaa aaatgctgtt ccaaaggtag tggcagaaac aatagaaagt
     6901 attccagtca tagtactgat tgcaaatccc agggctacag tgagaatagt aattaataag
     6961 aataaaatag caaattttcg aaactctcta tttcgaaaca taacttcccc ccaatctata
     7021 ccctgtccca cgaacagtca aaattatttg cggacttgaa gggtcatctt ctatcttctc
     7081 tcgcaagcgt ttgatgtaca cagtcaatgt gttgtcatta acaaactcac ctgctgcatc
     7141 ccacaattca tcaagtaatc tcacccttgt aataatactt ttaggattgc taataaacac
     7201 caataacaaa cggtattcta aagctgaaag aaaaacctca ttgccattct ttttcacaac
     7261 accggttgcc gtatctacat aaagcccaca aatttcaaaa gttgacccaa aacgcccact
     7321 ttttcgtagt gctgttctaa ttcgcgcaat cagttcacgt ggacgaaaag gtttagtaat
     7381 ataatcatct gctcccatat tcagcccagt gacaacactt gattcatcac cagaagctgt
     7441 caaaaatata acaggaatgt cttgctcttc tttgatttct gtgcaaatcg taaacccatt
     7501 tccatcaggt aaagaaatat caataagtgc caaatcaaat ttattcttag caatctctac
     7561 aagtgcttca cttcttgtgg atacatgggt tactataaac ccttctgaat taagcaaaag
     7621 aataagattt ttggcaattg ctttatcatc ctcaactaaa aatatccgtt tcatttactt
     7681 tcatcctcct ttattttatt agttttatat attccagcag agcaataata cactactatt
     7741 ttacttatta ttacacaatt ttcattagtt tcttctgttc ttatgtaatc tatgcataat
     7801 acctccattg atttattata tttcttataa cagaaataca caagatagga caaaaaacat
     7861 aatgtaagtg caactatttt ttaattcact aacttataaa cacatattcc aatgcacgcc
     7921 acctccctga tgatgtactt atcaatctac atgtttacta tggtactccc atgttagtat
     7981 acactttata atcaatactt gaaaagctaa gctcgatgtt ttattatata cttatatata
     8041 ccttataata taaggtatat tataatataa agtaggtgaa tctatggact tgaataaaga
     8101 agtacttaaa ggtcatattg atactttaat attatcaata ctaaataaag gggattcata
     8161 tggttatgaa atagcaaaaa cagttagaga tgaaacaacc tttgaattaa aggaaagtac
     8221 tctctatgta tctcttaaaa gactagaatc taagggtcta ataaaatcat attggggaag
     8281 tgaccaagga cttggaagta gaagaaagta ttacaatata acagatgaag gaaaagaaaa
     8341 tttagaatta aagattcaag agtggaactt tatacaaagc gttatgaaca aatttctaaa
     8401 gagaggggaa tagatttatg aaaataatag aagagtattt agaagcacta tataaaaatg
     8461 atactagcaa agaaattcaa gatttaaaag aagaattaaa agagcatcta atagattcca
     8521 ctaatgaatt tattgagaat ggatatgaca taaatgaagc acaaaataag gcaatagaaa
     8581 gatttgatgg cagtaatgga gtatcagtag aattacgttc aatctttaaa caaaaaatag
     8641 aacctaaaaa agtactatta cgaaaattat caaattcaag aattgtattt ttaaatttat
     8701 ttggatggct tcttgcagct actgcatata caataaatag aggttttggt catatagctc
     8761 caccatggct aattatactt ttaataatat caatagttat attaataatc ttaactatta
     8821 taattaagag attaaaaaag gaaataaaaa atgattacaa tatatagtta tacagttata
     8881 ttgtaatata ttatttcttc ttataaacat atattttgaa aggagctttt tatatggtca
     8941 aaaaaattct attagaaaaa tttcttgata cctcaggagt ttcatatatc aatgaaaaat
     9001 tgaatctatt taaggatact aagtggattt catttggcac ctatataatg accgttgata
     9061 gtttttgtta tagggaggat tcaaagcact ataacccaat aatgtctacc tttgattatg
     9121 aatcagagat ttttatagtt aatgccttat acaaggaaaa tatctattct attaaaagta
     9181 aaaaactcta taggaatgta tctaaaggag ataagataat tgttacaatt aaatactctt
     9241 atgataaata tgaaaatctt atacatgaag aaattcatga ctatcatctc tatcctctat
     9301 ctcttgatta aaataaaagg ctatagcctc tatagctaac acctttaatt taaaatactt
     9361 tgaatactat ataattttca taaaagactg taaataaact cgtatagaat ataaaattta
     9421 tctacagtct tttgttatac aaaatttata atctcttata gaacaaattt accacttttc
     9481 aaacttatta cttttactca gctatattat aaacttttaa tttatctttg tttaaatgtt
     9541 tcttcttaac ttctactaaa gcttttagtg tatccaaaga tgtcattatt tcagtagaaa
     9601 attcaatagc tgttcttctc atcttaaaac catctcttgt agagtcattt cctttagttg
     9661 gtgtatttat tactatatca acttgtttat ttcttatagc atctagtata tttggtctag
     9721 gttcttctac tctatttact gtttcagcct ctattccatt ttctcttaaa ctcttagctg
     9781 ttccttctgt agcaacaaaa gtatacccta attctttcat atcttttgct atctctataa
     9841 actcatcttt atcatgatta tttacagtag caagtactac tcctctttca tcagacatat
     9901 gtctacctgc tgacaagaac cctttatata aggcttcttc taaattttct cctacaccta
     9961 gaacttctcc tgtagacttc atttcaggac ctagacttac ttcaacttta gataatttag
    10021 acattgagaa tactggtact tttactgata ctaattttgg ctctttgtaa actcctgtac
    10081 catatcctaa atcctttaat ttagctccta acatacactt agtagctaag tcaactattg
    10141 gtactccact tactttactt atatatggaa ctgttctact agctctaggg ttaacctcaa
    10201 ttatatataa ttcattttga aattctataa actgtatatt aaccatacca agcacattaa
    10261 ggtctaaagc tattttttta gtgtattcta ctatcttttc ttttatttca tcagatatat
    10321 tttgacttgg atacattgtt gtactatctc cagagtgaac tccagctctt tctaaatgct
    10381 ccattatacc aggtatcaat atatcttcct tatcacatat cgcatctact tctatttctc
    10441 taccagttag atatttgtct ataagtactg gatttttgtg gtctctagca aacgcatcat
    10501 ccaaatactg aattaatttt tcttcattat aagttatttc cattccttga ccaccaagta
    10561 cataagatgg tctaactaat actggataac ctaactcctt agcaatctca actccctcat
    10621 tagttgtcca tacacctttt ccttttggtc tatttatatc tagtttttct aacaagtcat
    10681 caaacttctc tctatcctct gctgcatcta tatccctaaa gtctgttcct aatataggta
    10741 tatttttctc atgtaagaac ttagctaatt ttatagctgt ctgtccacca aactgtagta
    10801 ttacaccttc tggtttttct ttttctatta tatttaaaac ttcttcttct gttaatggtt
    10861 caaaatacag cttatctgat gtatcaaaat ctgtacttac tgtctctggg ttattattta
    10921 caattatagt ctcaattccc atttttctta aagattttac acagtgaact gaacaatagt
    10981 caaactctat accttgacct attcttattg gtccagagcc caatactatt acctttctct
    11041 tatcactaac tactacctca tcatattgtt cataagttga atagtaataa ggtgatagag
    11101 cttcaaattc tcctccacat gtatcaacca ttttataaac tggttgtata ttatataaac
    11161 ttctcaattc atataatttt tctgggctta tcttcattaa gtcagatata cctttatcag
    11221 aaaatccctt tttctttaat tcatgtaaat attctttatc taagtcttct atttttagaa
    11281 cttttaattt ttcttcttgt tctactatcc atttaaattt atttataaac cacttatcaa
    11341 caccagttat ttctgatatc atttctactt tatagcctct tcttatcatt tcagctaagt
    11401 caaatagtct ctcatcatca ggtactacta ctctagcctt taattcttct aatgttctac
    11461 cttctgatgg agcatgaacc aatgaatatt ttcctgtttc aagtgatctt atccctttaa
    11521 gaatagcagc ctcaaagtta cttcctatac tcatgatttc accagttgcc atcatcttag
    11581 ttcctaattt tctatttgca tgtctaaatt tatcaaatgg ccactttggt atttttacta
    11641 ccacataatc taatgttggc tcaaaacaag cataagtttt tttagttact gcattttcta
    11701 tctcatccaa agtgtaacct aaagctatct tagctgctac ttttgctatt gggtatcctg
    11761 tagcttttga tgctagggct gatgacctac taactcttgg gtttatttct attatcgcat
    11821 attctaaact atgtgggttt aaagctattt gaacgttaca tccaccttga acctctattg
    11881 catttattat gtctattgat gcctttctaa gtagttgata ctcttcatca cttagagttt
    11941 gacttggagc aacaactata ctgtctcctg tatgaactcc aactgggtct acattttcca
    12001 tattacatac agttatacag tttccatttc catctctcat tacttcatat tctatttctt
    12061 tccaaccttt tatactcttt tcaagaagaa cttgtccaac aggacttagt tgtagtccat
    12121 gtgagagtat ttctcttaat tcttcctcag tatcagctat acctccacca gttccaccta
    12181 gtgtgtatgc tggtcttaca actactggat atcctatctt attggcaaac tcaagacctg
    12241 cttctaaatt tgtaactata tcactaacta ttactggttg atttatttct ttcattactt
    12301 ctctaaataa atctctatct tcgccctttt ttatagactc tatagatgtt cctataactt
    12361 ttacattata tttatctaat ataccagcat catgtaattc tacagctaag tttaatcctg
    12421 tctgaccacc catacctgca agtaaactgt ctggtctttc tttttctatt attttttcta
    12481 taaattctat tgttaatggt tctatatata ttttatctgc aatttcttta tcagtcatta
    12541 tagtagctgg gttactattt actaatacaa cttcaattcc ctcttctttt aatgcttggc
    12601 aggcctgtgt tcctgaatag tcaaactctg cagcttgacc tattattatt ggccctgatc
    12661 ctaatactaa agttttcttt atactatcta atttaggcat aactctttct ccttatctaa
    12721 acaattttca cagttcttat cttcatcatc tatacaatca actttaatta ctttatttca
    12781 tcttaaattc aaatttactc atcaatacat ctatgttaat taaaccaaac tttttgattt
    12841 aagttattct tgttttttag ttagctattc taatgttatt tattttacta attctaaaaa
    12901 tttatcaaaa atatagtcat tatcttttgg tcctgggcaa gcttcaggat ggtattgaac
    12961 actgtataca tctagcttct catgtctcat tccttcaaca gtattatcat ttaaatttat
    13021 atgtgtcact ttcatttgtt caggaacttc tgaaacataa taaccatgat tttgagaagt
    13081 tataaatact tttccctctt ctaaatcttt aactggatga tttccacctc tatgtccaaa
    13141 tttaagttta gcagtttttc ctccaagagc taaagctaaa agttgatgcc ctaagcatat
    13201 tccaactata ggttttttac caataagagc ttttatattt tctataacat cttctaggtc
    13261 ttctgggtct ccaggtccat ttgacaagaa tattaagtca gggtttatac tcagtacatc
    13321 ttctggagat gttgtagctg gaaatattgt tatatcacat ccacgagcta taaatgatct
    13381 taatatattt ctttttactc caaaatccat aacagccact tttggaccat ttccttttat
    13441 atgctctact tcttttcttg taacagttct aactgcttct gtatttgaaa acttatccaa
    13501 tttatgttta acatcttcta atttactgtc ttctaatgta attatacctt tcattgttcc
    13561 attatttcta agtatcttag tcaaagctct tgtatctatt ccttcaagtc cgataacttt
    13621 attttgcttt aaatattggt ctatatcaat ttcacatcta aaattacttg gagaatcact
    13681 tttctccctt actatgaatc cctttacttg aactttttca gactctgctg atgataaatt
    13741 tataccataa tttcctacaa gaggataagt catagtaact atttgaccat agtaagaagg
    13801 gtctgttaaa acttcaccat atcctatcat cgaagtatta aatactactt ctcctacact
    13861 ttcttctaaa tatccaaatg cctttcctat aaaaactgtt ccatcttcta atattaacct
    13921 tgctttcata ctacagtacc tcctataatt ctctttaggc aaactaaaga acattaacta
    13981 aatataagct tttaaatatc tatatctatt ttttactttt acattctagt tttatactta
    14041 tttacattta ttttttatct ttttctatta tactacaaat ttcttaaaaa aaagaagtta
    14101 tctcaaaagt aaaaactcct agaaaaaata acctcttttt ttgatttaat aattaaattc
    14161 ttaaactata caatcagctt aatttctatt cagctatatt gtaaactttt aactcatctt
    14221 tatttaaatg tttcttctta acttctacta aagcttttag tgtatccaaa gatgtcatta
    14281 tttcagtaga aaattcaata gctgttcttc ttatcttaaa cccatctctt gtagagtcat
    14341 ttcctttagt tggtgtattt attactatat caacttgttt atttcttata gcatctaata
    14401 tgtttggtct agattcttct actctattta ctatatcagc ctctattcca ttttctctta
    14461 aactcttcgc tgttccttct gtagcaacaa aagtatatcc taactctttc atatcttttg
    14521 ctatctctat aaactcatct ttatcatgat tattaacagt agcaagtact actcctcttt
    14581 catcagacat atgtctacct gctgccaaga atcctttata taaggcttct tctaggtttt
    14641 ctcctacacc tagaacttct cctgtagact tcatttcagg tcctagactt acttcaactt
    14701 tagataattt agacattgag aatactggca cttttactga tactaatttt ggctctttgt
    14761 aaacccctgt accatatcct aaatccttta atttagctcc taacatacac ttagtcgcta
    14821 aatcaactat tggtactcca cttactttac ttatatatgg aactgttcta ctagctctag
    14881 gattaacctc aattatatac aattcatttt gaaattctat aaactgtata ttaaccatac
    14941 caatcacatt aaggtctaaa gctatttttt tagtatattc tactatcttt tcttttattt
    15001 catcagatat attttgactc ggatacattg ttgtactatc tccagagtga actccagctc
    15061 tttctaaatg ctccattata ccaggtatta atatatcttc tttatcacat atagcatcta
    15121 cttctatttc tctaccagtt agatatttgt ctataagcac tggattttta tggtctctat
    15181 caaacgcatc ttgtaggtat tgagataatt tttcttcgtt ataagttatt tccatacctt
    15241 gaccaccaag tacataagat ggtctaacta gtactggata acctaattca ttagcaactt
    15301 caataccttc gttaagtgac catacaccct ttccttttgg tctatttata tctagtctct
    15361 ctagtaaatc atcaaatttt tctctatctt ctgctgcatc tatatcatca aaatctgttc
    15421 ccagtatagg tatatttttt tcatgtaaaa acttagccaa ttttatagct gtttgtccac
    15481 caaactgtag tattacacct tctggctttt ctttttctat tatacttaaa acttcttctt
    15541 ctgttaatgg ttcaaaatac agcttatctg atgtatcaaa atctgtactt acagtttcag
    15601 ggttattgtt tatgattata gtttctatgt ccatttttct taaagatttt acacagtgaa
    15661 ctgaacaata atcaaattct attccttgac ctattcttat tggaccagaa cctaatacta
    15721 tcaccttttt cttatcacta acaactacct catcatattg ctcataagtt gaataatagt
    15781 aaggagatat agcatctatc tcacctgcac aagtatcaac cattttatat gctggtttga
    15841 tattgtatag acttctcaat tcatataatt tttctggact tatcttcatt aagtcagata
    15901 tacctttatc agagaatccc tttttcttta attcaagaag atagtcttta gttaaatctt
    15961 caattttcat aacttttaat ttttcttctt gctctactat ccacttaaat ttatttataa
    16021 accatttatc tatacctgta atttgttcta tcatttctac tttatagcct cttcttatca
    16081 tttcagctaa gtcaaatagt ctctcatcat caggtactac aactcttttc tttaattcct
    16141 caatacttct ctcctcagat ggtgtgtgaa ctaatgaata ttttcctgtt tcaagtgatc
    16201 ttatcccttt aagaatagca gcctcaaagt tacttcctat actcataatt tcaccagttg
    16261 ccatcatctt agttcctaat tttctatttg cttttttaaa tttatcaaaa ggccactttg
    16321 gtatttttgc caccacataa tctatagttg gttcaaaaca agcataagtt tttttagtta
    16381 ctgcattttc tatctcatct aaagtataac ctaaagctat ttttgacgaa actttagcta
    16441 ttgggtatcc tgtagctttt gatgctaaag ctgatgacct actaactctt ggatttatct
    16501 ctataactgc atattcaaaa ctatgtgggt taagtgcaaa ttgaacgtta caaccacctt
    16561 gaacttctac tgcatttaat atatctaaag atgctttttt aagcattgca cattctttgt
    16621 tacttaaagt ttgagttgga gcaacaacta tactgtctcc tgtatgaact ccgactgggt
    16681 ctatattttc catattacat acaactatac aatttccctt tgaatctctt attacctcat
    16741 attctatttc tttccaacct tttatactct tttcaattaa tacttgtcca acaggactta
    16801 attgtagacc atgtgaaagt atttctctta attcttcctc attatcagct ataccaccac
    16861 cagttccacc tagtgtgtat gctggtctta caactactgg ataaccaatt gtaagtgcat
    16921 aatcaagacc tgcttgtaaa tctgtaacta tatcactaac tattacaggc tgatttattt
    16981 ctttcattac ttctctaaat atatctctat cttcaccttt tttgatagat tctactgaag
    17041 tacctattat ttttactcca tatttgtcta gtataccttt ttcataaagt tcaactgcta
    17101 agtttagccc tgtttgtcca cccatacctg caagtaaact gtctggtctt tctttttcta
    17161 ttattttttc tataaattct attgttaatg gttctatata tattttatct gccacttctt
    17221 tatcagtcat tatagtagct ggattactat ttattaatac aacttcaatc ccttcctctt
    17281 tcaaagattg gcaagcttgt gttcctgaat agtcaaactc tgcagcttga cctattatta
    17341 ttggtcctga ccctaatact aaagttttct ttatactatc taatttaggc ataaattttt
    17401 ctccttatct aaatggtttt tactatctgt aaataatact aaatctatat taatgctaag
    17461 aatttattaa atatacaatc taaatcttct tttgttgtgt attctggaat gtattgaaca
    17521 ctatatattg gtaattcctt atgtctcatt ccttctatag tattgtcatt taaatttatg
    17581 tgagttatct ctgtattttc tggcatttct gaaacattat atccatgatt ttgagaagtt
    17641 ataaatacct ttccagtttc taaacattta actgggtgat tgcctcctct atgtccaaat
    17701 acaagctttg aagtctttcc tcctaatgcc aatgctaaaa tttgatgtcc taaacctatt
    17761 cctgtcaatg gctttttgcc tattaatttt tttatatttt caactatatc atttatatcc
    17821 tctggatttc ctggtccatt agataataag attaagtcag gatttatatt taaaatttct
    17881 tctactgtag tcattgctgg tactatagtt atatcacagt tacaagcttt aagagactct
    17941 agtacactat ttttaacacc aaagtctata actacaacct tagcaccagt accttttata
    18001 ttttcaattt cttttcttgt aacatttttt actgaatctt tgttacaatg ctcttctaat
    18061 ttagactgaa catcttgtaa tgatacattt tctaaagtta taattcctct cttagttcca
    18121 ttgtttctaa taattttagt taatgctctt gtatctattc cttcaagtcc tattacctta
    18181 ttttgcttta ggtatgtgtc tatatccatt tcacatctaa aattatttgg gttatcactt
    18241 ttttctctta caataagtcc ttttacttgc accttatttg attcaacatc ttctaaattt
    18301 attccatagt ttcctataag tggataagtc atagtaacta tttgtccata ataagttgca
    18361 tctgtcaata gttctccata cccagccatt gaagtattaa atacaacttc tccaatagtt
    18421 tcctttagat atccaaatgc ttttccttca aatactgttc catcttctaa aattaatttc
    18481 cccttcatgt aagctacctc ccaaatattt tatctaagca ttttctattt ctcttacaat
    18541 attaatagct gattctcttg gattttctgc ttttgttata ggtcttccaa caactaaata
    18601 atgagcacct ttttttattg catctgatgg agttacaact cttttttggt ctccaacctc
    18661 tgctgattta ggtcttattc ctggacaaac tgttataaaa tcagttccac atgcttctac
    18721 tattttatct acttcttgtg ctgaacaaac aaccccatct attcctgatt cttttgccag
    18781 tttagctctc tttatagcta actcttctgt tgtcatatta catcctatgt cctgtaaatc
    18841 ttggttgctt aatgaagtta gaaccgttac tccaactact aatggctttt ctatatttaa
    18901 tctttctgct tcttccctag caattagtgc acattgtctc attccttcca tatctgatac
    18961 atgtattgtc attaaccaaa cattatctct cactgcagct ctcactgcac ttttcatagt
    19021 atttggtata tcatggaatt taaggtctaa aaatattttt tttccttttt ctttaagata
    19081 atctattgtt ttaccttttg tggcaacgta ttgctctagc cccaccttaa atatgtctac
    19141 actatcttct aatctgtcta ttagttcttt agctttatca aattcgtcag tatcaattgc
    19201 aactatcaac ttttctttac catttatcat ttttacatct cctttttaat aaagcctaaa
    19261 taatcataaa aaaatcctca caccaaaaga ggcacaagga ttttatctat acataactct
    19321 attcattagc tatgagatat ctttcccagt ctctctggac tagtttaaaa gactttatta
    19381 tatttaaatt tttattttat actcatcaaa aacctctaga agatttacta gaggttatat
    19441 actaacacta tctataaccc tttagtctct ctggactact ttaaggaatt tagctgtaat
    19501 atttttcttt atttatataa ctttatgtat cactaggata atttaaagtt ttatcttttt
    19561 tataattaaa ccactaattg ctttttatgt caataagtct ttgaattatt cttctttatc
    19621 aaacaatttt ttctcaaaat atgattctgc attatatatt gcagcaactg gattatgccc
    19681 agttactcta tctttgacaa ctaaagttgt tataggagct tccatatatt tcatggctaa
    19741 agtatcgtga ccaacacata atccaagaat tacattaagg tcaaccttag cttcatttaa
    19801 aaataaagct tgtcctattg gattgcacat tacctcatct gaacactttg atattgtttg
    19861 agatgcatct agtccaattg tactcttagg cattgctcca tttttacata taacagaaaa
    19921 tacttcaaat ccatgatgtt caaatatttt ttgtactgtt tgcatttccc tttttagccc
    19981 aatacaaaat gctatgccaa tctttttata accacacttt ttagcaaact ctataatctc
    20041 ttttagtcta gtatattgac aatacccctc tgcttcaact aatccagcat tataagctat
    20101 ttttctattc tcttcttctt cataaagttg ctttgctttt tgttgtagtt caatttctct
    20161 agttggacat atcttcatta atttatccat atttccatta gaacagcctc taagattaca
    20221 ttttccacac ttatacatat ccaatatccc cttccaaaat tttattcatc ttgagattcc
    20281 tctttttcta ggattacatt ttcttcttgg atttcttctt ctggtttcac atgctcgact
    20341 acagttataa aatatgattt tcctatttta ttccttatag gaagtttttc atatgaaaca
    20401 tctattttat tttttataca agctatatca aaatgtctta aggctacacc taagctaacc
    20461 ttattcatat cttcacagtg tttcttaggt ctcttttcaa ataaatgaag acctcgttca
    20521 tccttatgga aataccacgg ttgagaattt tttacagatg gagctcttct tgtcaattct
    20581 aagatatctt tccactttct attcctattt atcttattta tcatatcttt tactggcttt
    20641 ctatcaggtc tagcatatct agtcctaaac aagtcttcat tttttgctgg atatccaaat
    20701 gctattatta tataaggatt ttctattttg ttttcatcat cttcttctgt ttttattcca
    20761 gctagataat ctaaatcatc ttcctcatct tcttcaaaat tcttattccc aatatctaca
    20821 aattccaaga tatcttctct ctttaagttt ccttccaacc agcaagtagc tattcccaat
    20881 gttgtaaggc gcaaaactac accttctgta gcaaatccta tattctgtaa atagtcctca
    20941 cctttatctg aggtaactac tatataatga ggtgccttta ctttacattc cttacccatc
    21001 aaaaaatgta ttatatgacc tctatcaaca acatgtgctt taatatttaa atcttcattt
    21061 aagtattcta aatttttaca acttcttttt atttcttcca tcaatggctt ttttatcttc
    21121 ttactggaaa aatttcttac agattttcta taaaaaatgg cttcataaag ctccatttat
    21181 gccctcctaa ctgaaatctt ctgacgtata aactctataa atttatataa atactttata
    21241 ttttttatag ttttatttcc tttttcctcc tgccctattt tgaaattcat aagaatatct
    21301 actctagttt aatatagcat tcaaaaggct taaattctac cttatcaatg tctatacatt
    21361 agcataattt ttttatttga ttttctatgc taatttaaat aacaacacta gctattaact
    21421 ttttaattaa gctttgccag aaaaattttt ttcatttata aatatctatc tcattattat
    21481 attataagtt tttttcggta ttcttgtata gcttttcact aaaaaagtat ttttatttct
    21541 actttaatac tattatcagc aaaaaacata ctgcttaaat aagtttgtta tcctcataat
    21601 ctgttttaat taaaatattt tttttatttc tcaatctata aagtataaat attttgaata
    21661 tgacccaaaa tctaagtaaa gttataatat tttataagtt gttcaatatt aaaaacataa
    21721 gtaatgttat aatactttat aagttattca atatcaaaaa tataggagga taaatatgaa
    21781 tttaaataga aatttagaaa aatacgctga attagcaata aaagttggag ttaacataca
    21841 gccaggtcaa attctactta taaagtcacc aatagaatgt gctgattttg caagaaatgc
    21901 acttaaagaa gcatataaat gtggtgctaa aaatgtttat atagagtggt ctgatgaaga
    21961 aagtactctt attaaatact tatatgctcc agatgaagca tttcatgagt tccccaaatg
    22021 gacagcagaa caatatgtag atatagcaaa agaaggtgga gcatttttat cagtctatgc
    22081 ccaaaatcca gatttattaa aagatgttga ccctgaaaga gttgcaaatt ttcagaaggc
    22141 atccggaaaa gctttaaaag aatggcgttc ttatactcta actgataaat gtaaatggag
    22201 tatagtctct gtgccaacaa aagattgggc aaaaaaggtg tttccaaatc tatcagaaga
    22261 taaagcaata gaaaaactgt gggatgctat atttaaatgt tcaagagtag atggtcaaga
    22321 ccctataaaa gcatgggaag aacataatga aaatcttaaa tctaaaatgg actttctaaa
    22381 taaaaataac tttaaaactt taaaatataa atcctcaaaa actgatttga ctctagactt
    22441 gccaaagggt catgtttggc taagtggagc atccaaagac cctaatggaa taagttttaa
    22501 tccgaatatt ccaactgaag agatttttgg aatgccacat aagtttaaag taaatggaac
    22561 tgtttatagt acaaaacctc ttgtttatgg tggaaatata attgacaatt tcttcttaac
    22621 atttaaagat ggtaaaataa tagatttctc tgctgaaaag ggactagata cactagaaaa
    22681 acttattgaa acggatgaag gctctcatta tttaggagaa gtcgctcttg ttccatacaa
    22741 ctcaccaata tccaatacag atataatttt ttataatact ctatatgatg aaaatgcttc
    22801 ttgccacttt gcaattggct ctgcttataa gacttgtctg gaaggtggaa acaatttaaa
    22861 agatgaagaa ttagatgaac atggtgtaaa tgacagttta acacatgtag attttatgat
    22921 tggttctgct gatatggata ttattggtga gacttatgat ggaaagcaaa tacagatttt
    22981 taaaaatggt aattgggctt tttaacatat aattttaact aaaaagggct gatggaagtt
    23041 ataccagact tcccagccct ttttaatgtt gatgataaat attttttata ttaattaatc
    23101 tcattattat agattaattc ctattgtttt aaacataagt atctaaaact tatatttata
    23161 ttatatataa aatatcctat ttagtaaaac ctttatttta atttacatgc taaaaattta
    23221 actttgctat ttcaacagca gactttgcat cctttgaata ataatcagca cccatttctt
    23281 cagcgtattc ttctgtcaaa actgcacctc ctacaaatac ctttgcattt atctcattat
    23341 ctctcagtgc ctgaattgta tctttcatac tttgaacagt cgtagtcata agtgcactta
    23401 atcctactaa ttttatattt ctctttttag caacttctac tacttcttca atagggacat
    23461 ctttacctaa atctataacc tcatatccat aattttctag cattattttt actatatttt
    23521 tcccaatatc atggatatca ccttttactg tagcaactat tatttttcct ttagatacat
    23581 tattgctact tttacttaat aaagtttcct ttattgtatt taatgaaact tttaccgttt
    23641 ctgctgattg aatcaattgt ggtaaaaata tatctccact atcatatctt tttcctactt
    23701 tatcaagagc aggaattaaa atctcatcta atatataatt ttcgtccttc tcttttaaaa
    23761 gattcagtgt tatatttttt gcttcatctt ttaatcccct ttcaactaaa atatccaagg
    23821 gcaaatcttt gtcaatcttt gtagatgaat ccgatttttt tgaattattt atattactat
    23881 attgatttat atagtttata catcctttat ctgtgttatt taatatttta aaagaattga
    23941 tagcttccat catactatat tcatttggat ttataattgc taaatcaagc cctgccccta
    24001 aagctaatgt tagaaaagaa gcattaagtg ctttcctatt tggaagacca aatgaaatat
    24061 ttgaaacacc taatatagtt tttactccta gtgtctttac cattgttata gcttttatag
    24121 tttctattgc ttcttcttgt tgtgctgaaa cagttaatga taaacaatct ataaatatgt
    24181 cttttggttt tatcccataa gaaactgctc tattaactat cctttttgct atttcaaatc
    24241 tttcttcagc ctttttaggt atcccctttt catcaagtgt aagtccaacc acacatgaac
    24301 catacttttt aactattggt aatatgcttt ctaaggactc ctctttgcca tttacagagt
    24361 ttactattgt tctaccatta taatatctaa gtccttgctc taaggcctct acattggaag
    24421 aatcaacttg taaaggtgta tctactactg cttgaatctc tcttattaat tttggcatta
    24481 gttttttttc atcaatctct ggaagaccaa cattcactcc aagtatttgt gctccagcat
    24541 ttacttgttc taagcctaaa tttatagcat aatcaacatt ttcatttttt aatgcttctt
    24601 gtagagattt tctacctgtg ggattcaatc tttctccaac aactgtaggc gattgtactt
    24661 ccacaaattt tgatggtgaa cagactacac attcatcaat tttctcgata catccctttg
    24721 taacagaatt tatattctca gatatttctt ttataaaact tggattcgta ccacaacatc
    24781 cacctattat agtagcacca acctcaacga actttttaac cccttcaaaa aattcttttg
    24841 catcaacatc atatatagcc tctccatcaa ctatatttgg aagacctgca tttgcttgaa
    24901 ccataacagg gactgtagct cttgatgcta ttttttcaac tattggtaaa agttgtttgg
    24961 gtcctaatga acaattaact cctattgcat ctacacctag accttctata gtagccacca
    25021 tacattctgg catacatcca gtaaaacttc ttccaccttc atcaaatgtc atagtacaga
    25081 aaataggtaa atcagaattt tctttagctg ctaacacagc tactttagct tcatataaat
    25141 ccatcatagt ctctattact atcacatcaa ctccagactt aacgccttgt agtacttgtc
    25201 ttttaaatat ttcatatgcc ttatcaaaac ttaaagtacc cataggctct agcatttctc
    25261 ctataggacc aatatctaaa gctacatatc ttggtttagt attatctaca ttttctatag
    25321 cttttctagc caccaaaaca gcattgtcaa tcacttcttc tacagtatac ttactatcta
    25381 attttagttc attacatcca aaagtatttg taagaattac atttgaacct gcctctaagt
    25441 atgctgtatg tatttctata agtttatctg gattttgaag accaaatact tctggatttt
    25501 cacctaactt taaccctttt tgttgaagca ttgttcccat tgcaccatca aatattaata
    25561 tattattttt cagatattgt cttatttcca caagagttcg ctccttttct atatgtacag
    25621 tttctatagt ttttacaatt ttcacacgac ttttctttct tagatttatt tattcttgta
    25681 ttagaaatac ctataatagc cactactgac tttctcggta tcataattcc attagaagta
    25741 attgttagac ctatttgttt ttgagcattc aaaacgttta atatatctat atttttttct
    25801 attggtaaat ctccatatcc tgggctatat cgcatagtta taaatttatc ttgctttaaa
    25861 aaaacctttt ctaaattatt ttgaacaatg tcacacactt cttctattgc tgttgttcca
    25921 catgaatcaa ttattagtcc ttttgttaaa tttgaataag agtgtcgtct tatttctttt
    25981 tctatatcta tccctatagt agctgccata attacacact cattacattc ttttaaaagt
    26041 tcatatatat ctttactctc taaggttaag tttgttccct ctaattctac aatattatct
    26101 ttatttattc taataggata aatcctagat aaatatctag gatttatttt atttttagtt
    26161 atagctacac attcatcaat agtatattta aggtctttat ctatctcttg acctctgtat
    26221 tgtaaatatc ttaaaacttc actttgattt atgcttatat cttctcttcc aattaaatta
    26281 ttctccatat atatttaaag aaaatccttt atctaaaaca tcttttgcat attcctttgc
    26341 cccaaataaa gataagtctc tgtagtttcc acattctaca gctgtagctg ctggcatttt
    26401 atctgattca agaacttttt ttaatgcttc ttctaaacca atcttaactg ttttggcatc
    26461 tacatctccc catagtatta aataaaatcc tgttctacat cccattggag ataagtctat
    26521 tacaccatct aatgtatctc ttaaataagt agctaaaaga tgctctaaac cgtgcatagc
    26581 agcagttcca aaactttcta catttggctg taaaaatctc aaatcaaatt tagttacttt
    26641 atctcccttt actccatcaa gtacactaca ctttcttaca aaaggagctt ttacttttgt
    26701 atggtctagc ttaaaacttt ctactttttc cattatttaa tcctccatta tactttaaaa
    26761 tttgttacat taattctaaa aaaaactctt cctatctatt attgataaga agagtatatt
    26821 atttcaagtc atctcatcgt tgttagcagg accacctgat aaaaggttgg tataggatca
    26881 ctgagctaac tctctcccct aactcttgat aagtatctta ttaaactgac ttaaattata
    26941 tattactttt ttatttttgt aaacacttta ttgttataat atttatatca cataatcgta
    27001 cttttatttt ataactaagc tgattgtatt attggcagtt gtacctcaaa tacagtttga
    27061 tatttatcgc ttgaaacagt aatttctcca ttatgtaaat ttacaatatt ttttgtaatc
    27121 gcaagaccca atccagaacc tccaacattt ctatttctag atttttcaac tctataaaat
    27181 ctatcaaata tatttggtaa atctaacaca ggaattggtt ctccataatt tatcactttt
    27241 acaaccgcca tatcttcctt tttctctgtg actatatcta cataatggcc atcttttcca
    27301 tatttcacag cattagttat aagattttca aatgctctta ccaatttatt tggatcagct
    27361 tttacaatta atttttcttc attaaaatta attctactaa ccataaattc ttgtttaaaa
    27421 taaatttcaa attgagatac tacttgactt aataactcta ctaaatttat ttctattttg
    27481 tttaatttta ttgtattatt ttgcatttta gtcaattcaa acaaatcatt tataagaaca
    27541 tttaaatttt tcgatttttc atatgcaata tcaacataat acatcatctg aacttcatct
    27601 ttatacttac cttcttctat cactcctaaa taacccatta tggatgtcaa aggtgtcctt
    27661 aagtcatgtg acacatttgt tattaaatct gtttttgtct gctgagcttg tctctcttca
    27721 atagttatat ttctaagttg atggactata ttattgattt tatgtgctag ttcatcaata
    27781 tcatcctttt ctttatcctt taatatatct aagtcatact caatatctat acttttttct
    27841 aagttacctt catccattaa atttacagca cttttaatga tttctaattt ttgatttttt
    27901 ctattagtct ttcttataaa atctacaatt tgcttatttc tcttgtatga tatcatggaa
    27961 taaattgcta atgatgtgat tgtaaataaa aatggataaa ataaactctc tcctataaga
    28021 cttctgaaat ctcttaactg atatacaagt tgattaatta cagatatctc actattgaaa
    28081 tataataatt ttattatata catacttgtt aaaataagca ttgaagttaa accacagctt
    28141 attataagat ctaatatggg tcgaaacata tgatttaata aacttttatt tttcaatttt
    28201 atatcctact ccccatacag tatgaattat tttttctcca tctgtatatt tttcaatttt
    28261 atcccttatt ctgctcatat gaaccattac agtattatta gattggtaat atttctcttt
    28321 ccataccttt tcaaatattt cttctgtact atatactctg cctttattgt ttgctagtaa
    28381 atataatata tcaaattctc ttgatgtcaa aattatttca tttccagcta ctgttacact
    28441 atgtttttcc ttatctataa ttactactcc aacttctatg acatctttat cttcattatt
    28501 agaaccatta aaataatatg ctcttctaat cagtgatttc actctcacag taagttcaag
    28561 tgggttaaat ggttttgtca aataatcatc tgcaccagtc attatccctt gtattttatc
    28621 catgtcttct gattttgcac ttaacataag tattggtata ttcaaacttt ctctaacttt
    28681 tctacaaact tctatcccat ccattttagg catcattata tccaatacca ttaaatgaat
    28741 attttcattt tttaaaatat ctagagcctc ttttccatct tcagctttaa ttacattata
    28801 tccactattt aaaagatata tctctataag gtctcttatt tccttttcat catccacaac
    28861 aagaactgta ttgttcattt ttacacatcc taacttcatc tttgtatata actttactca
    28921 tataaaatta tactatattt tagttagtgt aatctataaa tttattcatt tctaaatttc
    28981 caatataata caaattattt acacatttat attatttatt ttgtaaatat tcttctaaag
    29041 ttaatccttt tgaataaatt tcctcggcag catccttacc aacatatcta atatgccatg
    29101 gttcatatgc tgtacctgtt atatcttctt taccttcagg atatcttatt ataaatccaa
    29161 acttgtgagc attttctgcc aaccatttag cttcttttgt acttccaaca aaccatcttt
    29221 ctggatttgt taaatcaata caaagtccag tttgatgctc actgtaacca ggctttgcta
    29281 catatgcatc tgccttttct cgcccctgag attttaccct tcttgtataa gtatccagtt
    29341 gagtatcata agatctgtat gctgaagacc ctaaaaactg tattccttct gaattagctt
    29401 gcttaactaa atcttcaact gcctttgcag gttctcctgc catttgtttt tcttcctctg
    29461 ttgcttcttc aacaaaaggt atgtttacct tagtaatatt ttcaggagta tagtttttgc
    29521 taattccatt tgtcttgttt accaataata tatttgatat actatttgag tgatttttat
    29581 tttgagaatt tgcctcaaca tcttgttctg aatcatgttg agcaactact attggatctt
    29641 tatctttctt aagtaatctc tctttagata taaatacact tataaacact actaaaataa
    29701 tggctcctat taatttaaat tttttcataa ttacatattc tcctttttgt tatgtttgtc
    29761 tataatgttg attttagagt ataagactta agtattttat aagtaaatct taaataaatc
    29821 ttaaatttaa tatttttaaa ggatggtgat aaaatgtcta tacaaaaact tctaagtaaa
    29881 gctcgtaagg ctatacaaga ttttgatatg atacaagaaa atgataaaat tgcggttgga
    29941 ctttctggtg gaaaagatag tttaactcta cttcatatac ttaaaaatta tcaaagattt
    30001 tctccacaaa actttgaatt gatagccata acattaaata ccggaggtgt tgataattct
    30061 ccactagata aattgtgtaa agaaataaat gttccttttt atgaatttca aactgacata
    30121 aaagaaattg tatttgatat aagacaagaa aagaatcctt gtgcattatg tgctaattta
    30181 agaagaggtg ctttgaacga taatgctaaa aaacttggat gtaataaagt agctcttggt
    30241 catcataaag atgatgcaat tgagacattt ttaatgtcta tgttttatga gggtagagta
    30301 aattgttttt ccccaaagac atacctagat agacaggatt taacagttat aagacctatg
    30361 atttacatag aagagtatat gactaagaaa atttcaaaag attctaacta tcctattatc
    30421 acaaatccat gccctgcaaa tggtcatact aggagagagt acataaaaaa tcttattgct
    30481 aatttaaata aagagatgcc tgattttaaa agaaatgtat ttggagcttt aaataattct
    30541 gagaaattgt ttatatggga taaagagaag ataaaaaatt ttaaataata attaaaattt
    30601 ttaaataaaa aagcaagatt gtattaattg taaataatct ttat
//
LOCUS       DAEMAE010000016        30392 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEMAE010000016 DAEMAE010000000
VERSION     DAEMAE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.016"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(18..653)
                     /locus_tag="KPH93_000658"
     CDS             complement(18..653)
                     /locus_tag="KPH93_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG1687495.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(733..1464)
                     /locus_tag="KPH93_000659"
     CDS             complement(733..1464)
                     /locus_tag="KPH93_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG1687496.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILVSPTD
                     LILNIELKTDKVNYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDSSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDIIHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            1795..3984
                     /locus_tag="KPH93_000660"
     CDS             1795..3984
                     /locus_tag="KPH93_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG1687497.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTINIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNDNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVKSEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINNEEMFKLGYELTEKITPSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSINATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
     gene            4395..6323
                     /locus_tag="KPH93_000661"
     CDS             4395..6323
                     /locus_tag="KPH93_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG1687498.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSTGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            6310..6657
                     /locus_tag="KPH93_000662"
     CDS             6310..6657
                     /locus_tag="KPH93_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG1687499.1"
                     /translation="MSKSNDVLDIVFKITDELEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(6905..8824)
                     /locus_tag="KPH93_000663"
     CDS             complement(6905..8824)
                     /locus_tag="KPH93_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1687500.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINKDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKVLEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTSKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8850..9656)
                     /locus_tag="KPH93_000664"
     CDS             complement(8850..9656)
                     /locus_tag="KPH93_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1687501.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKDNIKSQETMQSKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(9715..11163)
                     /gene="ddlR"
                     /locus_tag="KPH93_000665"
     CDS             complement(9715..11163)
                     /gene="ddlR"
                     /locus_tag="KPH93_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG1687502.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGVDISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVLYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCVKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(11187..12086)
                     /locus_tag="KPH93_000666"
     CDS             complement(11187..12086)
                     /locus_tag="KPH93_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG1687503.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            12344..13600
                     /locus_tag="KPH93_000667"
     CDS             12344..13600
                     /locus_tag="KPH93_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG1687504.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKNGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKIRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            13670..14449
                     /locus_tag="KPH93_000668"
     CDS             13670..14449
                     /locus_tag="KPH93_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1687505.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIIAIEEIEKKGVQFNVATGRI
                     FKQARNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKARGDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(14543..15127)
                     /locus_tag="KPH93_000669"
     CDS             complement(14543..15127)
                     /locus_tag="KPH93_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG1687506.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKRTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYNIANCG"
     gene            complement(15327..16757)
                     /locus_tag="KPH93_000670"
     CDS             complement(15327..16757)
                     /locus_tag="KPH93_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1687507.1"
                     /translation="MELDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQAGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYGFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17085..18029
                     /locus_tag="KPH93_000671"
     CDS             17085..18029
                     /locus_tag="KPH93_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG1687508.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVSGGRLDFQGLNAFGLFLGT
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYATGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            complement(18300..19235)
                     /locus_tag="KPH93_000672"
     CDS             complement(18300..19235)
                     /locus_tag="KPH93_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG1687509.1"
                     /translation="MDKFKYAFNNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQSCKFV"
     gene            complement(19355..19948)
                     /locus_tag="KPH93_000673"
     CDS             complement(19355..19948)
                     /locus_tag="KPH93_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG1687510.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            20055..20483
                     /locus_tag="KPH93_000674"
     CDS             20055..20483
                     /locus_tag="KPH93_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG1687511.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            20550..21218
                     /locus_tag="KPH93_000675"
     CDS             20550..21218
                     /locus_tag="KPH93_000675"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG1687512.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(21336..22691)
                     /locus_tag="KPH93_000676"
     CDS             complement(21336..22691)
                     /locus_tag="KPH93_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1687513.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASIAVFAICM
                     AFSEPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(23195..23689)
                     /locus_tag="KPH93_000677"
     CDS             complement(23195..23689)
                     /locus_tag="KPH93_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1687514.1"
                     /translation="MLNSAKNQVGFIKSVLKESYRLKEISENICDFVTTLIKKNSSTS
                     KFYTKQKAQLLLEKSKNIPIGIPIFLMLTLGLRFGEAVSLIWSDVDLDKKIIHVNQTL
                     VYVDNKIIFKDPKTPKSKRKLFAPDELISLLEEEKFKQNKLKLQAILKNEFDLICLNK
                     RFNP"
     gene            23785..24141
                     /locus_tag="KPH93_000678"
     CDS             23785..24141
                     /locus_tag="KPH93_000678"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1687515.1"
                     /translation="MVFETFDKVIELYPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIENYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
     gene            complement(25220..26953)
                     /locus_tag="KPH93_000679"
     CDS             complement(25220..26953)
                     /locus_tag="KPH93_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1687516.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGKYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLEFGKIIHLDDIGKLLEVLHGNLRETIEIRQINSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIQEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(27190..28035)
                     /locus_tag="KPH93_000680"
     CDS             complement(27190..28035)
                     /locus_tag="KPH93_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1687517.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKPYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLETLRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(28066..30300)
                     /locus_tag="KPH93_000681"
     CDS             complement(28066..30300)
                     /locus_tag="KPH93_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1687518.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFNFKDNSFYNKLENDKEFLSEISYV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRKWIDKNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYNVSTSNFSIE
                     ITENILIENTNTIIQQLTELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQYMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEAAKHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNVLSQ
                     FYSMLPVGIIRYDLFGDMIITYINEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYIDINKIKN
                     LTKI"
BASE COUNT        10765 a         3946 c         4286 g        11395 t
ORIGIN      
        1 tataaagtat tagaggatta acgaatctct tttaatacat taactccatt ttgaatcata
       61 ctgttgaaaa atactaagtt taaaatctca gcagggtgaa cgttaggaat atcataagta
      121 tctactaaat tcataggtac aactatattt ttttcaatat tatgttgatt aaaatatgat
      181 tttagagtta ttgcaaattg atatatacat atatctgtgc aatcgccaac tataattata
      241 ttatctatat tatcaaaatc caaattttct aatgcaaaaa atccattagt agaattttta
      301 ggaagaattt gtaggttttc tatcgactta agttcatcaa ccaattcact ttctacatca
      361 ttttccaaac agtgaacagg ataactcaaa agttctattg atttaggagt atgagaatca
      421 gtaaatgcta ttactctatt taatttgttt gatatttttt ttgtaaacat ttcaattggc
      481 tttattaaag actctactct aggagagtat aatgcacctt gtcttgcaaa gccattatta
      541 acatcaatta taaataaagc agttttactc aaatcataat tttctatttt ttctattggt
      601 agattatcta aattactctt taaagcttca agttcattta ataagttatc catagttata
      661 cctccaagat aaatttaata aaattgtaca tattaattat ataaaagtca aggagtacaa
      721 actattgtgt aattagctat attgttttga gtgtaaaaga tttgaagcaa tatctggata
      781 gttagtaaaa ataccatcta cattgagttt tagacagtgt attatatcat cctcttcatt
      841 tacagtatat acattgacac caagtagatt tttgtgacaa agttcaatta gagattcatt
      901 tactaaaact agaggtggat gaaggtaact attacccaga aatttagcaa aattgactac
      961 attgtttata ggtaaataac acaataatcc tgtttttaca ctagagtcta atttatgaaa
     1021 gttttttaaa ctattgctgt tgaaactaga tataagaact ctatttttca tattatactt
     1081 taaaataagt tctaaaacat ctttttctat attgggatag tttactttgt cagtttttag
     1141 ctctatattt aaaataaggt ctgtaggtga aactagaatt aatacttctt ctaaagttgg
     1201 aattttacat tctttattgt tttgatatcc ttcaaaagat gagtttagag tttttaattt
     1261 tcttaaagta aagtctttta caaaaccatg tccattaaaa gttctatcaa ccttttcatc
     1321 atgaataact acaatttttc catccttagt tctgtggaca tcaagttcta ttccatatat
     1381 atccatatcc aaacattttt taaaagctag aatagtattt tctggatact ttccactaaa
     1441 ccccctatga gcataaatat tcatttatgt ggcccctttc gataaatata acttatctta
     1501 taaaaattta attaatataa tatatcatat tccaaaaggt attgttatat attaattaaa
     1561 taactatttg taatttatat attatataaa tgtagaaaaa taaaatcata tatgtgttat
     1621 aatatatttt aaacattgta aatattacga atttagtagc cataataagt gtaattttat
     1681 gcaagctcat gtttttagag cttgcatatg tattattaat atatttatat attgtgaaaa
     1741 taaaattcca aaagaatttt ttctatagac ttagtagtaa gggggaattt tgatatgtat
     1801 gaagttaatt tagaacgagc tatgaattat tggatggatt tgttagatga ttatacgaat
     1861 atagcaaaaa ttccaaatga ttttttttat aaagattgtt ctgatgaagt ggtagatgag
     1921 gtttttaaga tgaatattga gaaacatgaa aggttaattc aatattctaa agttaacaat
     1981 attagcatag actctatcat agaaactata tatggtctta tacttcaaaa gtatatatat
     2041 gaagatgatg tagtttttgg taaaacaata aatataattc caataagagt aagagttaga
     2101 caaagtgaaa aatttataga tgttgtaagg aagctaaaaa ggcagtggag caggtcttta
     2161 gagtatggat actatttgtt atcagatata gagaataaaa atcatctaaa tactaagctt
     2221 ataaacacaa tatttatagc taaaagtttt gatttatcat atgattatga catagaaaaa
     2281 aaaggatatg atttatctgt aatagtatat actaagaggg cacttaaaat aaaaataaag
     2341 tataaaccat ctatatacaa aagagagact attagaagaa ttttaaatca atttgattat
     2401 ataatagagc aaataataga agataatgat attttaatag aagatttaga atttgtatgt
     2461 aaggaagaag aaaaagtatt attagaagac tttaatgata atcaatatga agtaccctat
     2521 gataaaagtt atgttgattt atttagggag caagttaagg taacacctag taatatagct
     2581 gctagggatg aaaatagaaa gttaacatat aaagaattgg atgaggtaac tgataaactt
     2641 gcaggttatt taaatcatat aggagtgaaa agtgaagaca tagtagctgt aatgttacca
     2701 agagatatta acataattat tacagcaata gggattatga aatcaggggg agcatttttt
     2761 ccaatagaca caagtaatcc agaagaaaga ttgaattacc ttcttgaaga tagtaatgca
     2821 aaagtagtga taactacaga tgaactcaaa agtaaggttg taaatgagaa taccatagtg
     2881 ctagatataa ataatgaaga aatgtttaag cttggatatg aattaactga aaaaataaca
     2941 ccatcaaatt gtgcatatac aatatctacc tcagggtcaa ctggtaggcc aaagaccata
     3001 gctatagaac ataagagttt agtaaatatg tgttattata gcgtaaaatc tataaacgct
     3061 acagaaaatg atatatgtgg tatatactta agtttcagtt ttgatgctgt aatgaaacaa
     3121 ctattccctt atttgcttgt aggtgcatca atagatatta tgcctgaaga agctaaattc
     3181 gatgaatata cagtaaatga atattgtgag gaaaatgaca taactatact agcattgcca
     3241 acagcgtttg caaggttatt tatacaaaat tgcaataata attcacttag agttgtacag
     3301 actggaggag aaagattaaa aggatataga aagcgtaatt atgaactata taatgagtat
     3361 ggaccaaccg aatttactgt tgtatcgact tcttttcatg tggacagaga atatggaaaa
     3421 ataccaatag gaaaacctat atttaatact tatgcatatg tattggataa aaagaacaaa
     3481 ctatgtccaa ttggagctcc aggagagtta tgtctatcag gcattcaaat atcaagagga
     3541 tatttaaata aaaaagggct aacagagcaa gtatttgtag agaatcctta caaaacttgt
     3601 gaacataata agttaatgta tagaactggt gatttagtga gatggttaga tgatggaaat
     3661 ttagattata ttggaaggat ggacaatcaa gtaaaaattg atgagtttag gatagagcta
     3721 tatgagattg aaaatataat aaataatatt acagaaatta aaagtgttgt ttgtatatcg
     3781 agaacaaatg atgatggaga tatgtatata tgtgcatatt atgtaattga tgaggaagat
     3841 agtggtaaga taaatgaaag aactattaga gaatatttaa atgagcatct acctccatat
     3901 atgattccaa caataataat gaggatagat aaaataccag ttacaccaat aggaaaagta
     3961 aataaaaaag cactaccaga gtgatttttt attatgacag gtgttgtaaa gtgattccat
     4021 acatttcttt atgacaccta tttttctgag aaaatcaatt gatgtaaaaa aaagaatttt
     4081 ctgtatatat ttactatggt gaaaatttat ttataataat gattattgta ttttttatac
     4141 ttttccgtgt tttaaagcac tttttttaca aaattaattt tatattaaaa aattttttta
     4201 ttttatatct taatagtttt tagtataatt gaaaaataaa aagaaaatta gcataaaaat
     4261 atctaaaagt agaaattaat aatatagggt taaattttag tagttttgac tcgctatttg
     4321 taaaataata aaaagttaag ttttatatgt atatatgtta taatatatac aaattgaaaa
     4381 ggggtgaaaa ttttatgaac aaaaaattac ctaagggtgc ttatggtgag gttagtggaa
     4441 aagattacgt tccatatatc acagataagt ccagaaccgg tggaaatgta gctgtactga
     4501 ttatcggtat tattttagct gccatttttg ccgcatctac aacttactct ggaatgaaag
     4561 ccggtcttac agttgccgca ggtattccag gagctataat aggttctgct tttgttggag
     4621 catttgctcg ttcaaaaggt attcttggta aaaacttaat ccaaggtatg tcaagtggtg
     4681 gagaatctgt tgcaagtgga tttattttcg tattaccagc agttatatta attggaagtc
     4741 aaattacatt cttagaaggc ttagctgttg gagttggtgg agttttattt ggtataggaa
     4801 ttgctgctat agttcacaac tatctaatag ttgaagaaca tggtaaactt atgtacccag
     4861 aatcaatggc tatatctgag acacttgttg cttcagaggc tggtggagat tcaattaagt
     4921 acatgggaat tggatttgta ataagtggat ttattactgt attaactggt tcattcttaa
     4981 atgtagcaaa taatgtaatg agtcttgtag gaagcaaatt ctataaatgg aaatttgata
     5041 ttgaagtaaa tcctctactt ttaggaattg gatttatagt agggcttgaa gtttctttaa
     5101 caatgtttgc aggaagtatt ttatcaaact ttggtatagc acctttaata ggatacttta
     5161 cagatatggc taaagatgga gctatggtat ggaataatcc tgcaatgcct cttaaccaaa
     5221 tggatgttgg agcaatatct agtagttatg taaaatatat aggtgctggt atgatgcttt
     5281 gtggaggtat tattggtgca ataaaactta taccaactat aatcgcttct ataaaagaaa
     5341 cacttaaagc taaatcaagc actggagaag gagaagaagg ttcatctata caaatgatac
     5401 ttttgttagg tggagtagtt attggatttt tagcagcatt cttaatttct ggtaacattg
     5461 tcatggcaat tattggagct atcatatctc ttttattatc tttattattt gtcatagtag
     5521 ctggtcgttt aacaggaaca atcggaacat caaatcttcc agtttctggt atgactatag
     5581 cttctctagt tattgtaaca ttagtgtttg ttataatggg atggactgat ttagaagcta
     5641 ataaatcatt acttttattt ggttcattta tagttgttgc tattgctata gctggaggtt
     5701 atactcaatc tcaaaaagta acttatatta ttggtggtag caaaaatgaa atgcaacgtt
     5761 actttacaat tgcaagtata gttggtgtta tagttgttgt aggagtaatt ttattacttt
     5821 ctgaccaact tagagcaact ggtgataatg tacaatttgc attaccacaa gcaaacttaa
     5881 tgtcaacatt aacttctggt attatgtcag gtagtttacc ttgggttatg attattgttg
     5941 gagtatttat ggcaatcgtt ctatatgcat taaatttacc aataatgact atagctatag
     6001 gattctattt accaattgca acaacttcta taatattagt tggagcatta attcgtttat
     6061 ttgttgaatt ggtatctaaa actgaaaaag aaaaagaagt taaagtttct aatggtataa
     6121 gtttatcttc tggtcttgtt gctggtggtt ctatcattgg tttaataggt ataatattac
     6181 aagtaactgg agttgtaact ccaaaggtac caagtggatt tgctgcaaca aattcaatgg
     6241 caattgcttt attagtagtt ttagttgtac ttacagcatt acctattgtt ttatctaaag
     6301 taaaaaataa tgagcaagag taatgatgtt ttagatattg tatttaaaat tactgatgaa
     6361 cttgaatatg aagtagctga aaatagtgtt gttactatac ttgaaaaaca ggaccataaa
     6421 attcaaaagt tttttaggaa gttaaaagct agaataccta aatacaaaaa aatagaacta
     6481 gatgaatatt ccagttttgt ttttttacaa attgatggtc aaaggactgt tagagaaata
     6541 ggcaagaacc tagaagctaa atatggagaa gaatctcatc cactttatga aaggttgtta
     6601 gtatttttaa atcatattga agttaattgt cactatattg aaagactgga tatatagata
     6661 taataagaat tattatagat atattagagc atataaatta gaaggtaatt ataaaaatta
     6721 tgaaagaggc tgtctcaaaa tagagataag cctcttttgt attgttgaga tgattaaaat
     6781 atatttgatt tcacataaaa aagagtgtct cataaaacta aaaagttcat tttgagacac
     6841 tccctttaaa atatttataa agtacagatt tgtaaaactt attaaactta agattatttt
     6901 aatatcattt attattagct atttcatcag ctagattatt taaatattcc catctttcat
     6961 atttgtattc aagttctttt tctagtttag atttctcatc taaaatttct tgaagtttag
     7021 taaaatcagt tgaaaatttt gaagtacttt catcaagtgt tgcaatttta gattcgagct
     7081 tttctatatc agagtcaata ttttcaaact ccctttgttc attatatgag aacttaagct
     7141 ttttatcatt tttaggtttt tctttttttg gggtagtaga gtcatctttt ttaactgatt
     7201 cccttttaga ttcttcaaat tcaattcctt gaatctctct ataaataaga taatcactgt
     7261 aattacctgt atagatatga attttcccat taccttcata tgcaaatatt ttattacaaa
     7321 ttctatctaa gaaatatcta tcatgtgata ctgtaactac tacaccttta aattcgtcta
     7381 ggtattcttc taatacttta agagtttcaa tatctaaatc atttgtaggc tcatctaaaa
     7441 gaagcacatt aggagctgtc ataaggattt taagaagatg tagtcttctt ctttcaccac
     7501 cagataattt acctatcaaa gtatattgca tagtaccatt aaaaaggaat ctttcacaca
     7561 tttgagaagc tgtaatccta gtaccatctg aagtttctat atagtcacta gcttctttta
     7621 catagtctat agccctcata tcaatattca tatgagagtc gtcttgagaa aaacaaccta
     7681 ttttaaccgt ttctccaatt gaaatatatc cactatcagg ctctatttct ccattaagta
     7741 tgtttataag ggtagattta cccataccat ttttgcctat tataccaatt ctatctgtac
     7801 gagttaaagt atattctaaa ttatctataa cagtattttc ttcaaatgat ttagatatat
     7861 tgtgtatttc tattatttta tttccaagtc tgcttgatgc aacagatata tcgagatttt
     7921 catcaggttt aaaagagtct ttatttataa gttcatcaaa tctttgtaaa cgagcttttt
     7981 gtttagtgct acgagcctga gctcctcgtc ttacccacgc aagttctgtt cttattaaat
     8041 tttgtctttt gtcttcaata cttgcttcta gcgaaagtct ttccattttc ttttccaaaa
     8101 atattgaata attcccatca taactgaata atctaccctt atccagttca ataattctat
     8161 ttgtaacacg gtctaaaaag tatctatcat gagttatcat aagcaatgaa ccttttcttg
     8221 agttaagata ttcttcaagc caatctatag tatcattgtc taggtggttt gtaggctcat
     8281 ctagtatcaa aaactcacaa ggtgtaataa gggctgatgc cagagagact ctctttcttt
     8341 gcccaccaga aagttctgct attttttgat taaagtcatt tataccaagc tttgtaagta
     8401 ctgacttagc ttcactttct aaatcccaaa gatttaaggc atctattttt tcgtgtactg
     8461 ataataattt tttatttaat tcctctgtaa agtttgaatt aagtttttct aggatttctt
     8521 gatattcacc aagtagcttt aattcttcag aagttccatt aaatacttgt tcaagtacag
     8581 ttgaattttc attgtaagtt ggattttgag gtagatattc tatcctaata ccattagttt
     8641 ttgttatatt accagtctca ctttcttcaa ctccggcaat aatttttagt aaggtagatt
     8701 taccagtacc atttacacca ataagtccta ttttttcccc ctcatttatt ccaagagata
     8761 tattttcaag taattttttt tcagaatagc ttttacttat attttccaat gtcattaaat
     8821 tcatataatt cacctttatt tctaatctat tatttttcaa aatgttgata atctgggttt
     8881 ttccaataac caccccaact ccaacctctg cttacaaaag cattataaca atcatcacct
     8941 ttttttatca ttccaaattt tgtgctagac ctatcagcat aaactgtact tactttaggt
     9001 gtaacatcat ttccagaaac ttgaggattc tgtaaagggt ttatatctat agcacgtcct
     9061 ttaccatgat ttgaaacgac atttgtacca gctatagttc tataacaaaa tgatgaggta
     9121 ttattatcag acatagattt ttcatctaca gcatcatagt catctattaa cttaattttt
     9181 tcaattggat attttttttc gtataattcc ttaaaaatat ccactacttc tggagctagt
     9241 tcactgttta ctatcatttc tccttgatga gttttttcat caaatccata gtatgtaagt
     9301 tttaaataag atagactatc aaagcttata ggttcatcag taggcataga tttaccaatc
     9361 attttatttt ttatattatc tggtattgaa gaatatgaaa aagtaggctt gatattatta
     9421 tcttcctgct ttttggtaga gctttgatta tttttagaat catctgttgt attattttta
     9481 ctttgcatag tttcttgact ctttatatta tcttttttgt tgggattaga gaacagtatc
     9541 tcatatatag ataataaagc aagaaataat acagttatta caatcgttaa tttcaattta
     9601 ttaatttttc ttcgttttga ctttttactc atattatccc ccagtattag attcatataa
     9661 actatatata ttatacctca taaaaataaa ctaggctaac aatatttagt taacctagaa
     9721 catttcattt atagtttctc caattatttt tattccaatt tcaatgtctt caaaagacaa
     9781 cctagaaaat ccaagtctta aagtatcaaa accagagtta tctgtaaaaa ataaatctcc
     9841 aggcataaat ataacatttt tttcataaca tttttctaat aaaactctag tatctatatt
     9901 ttttagtttt ataaatatat gcaaaccacc atcacccaag atgtattctg tttttatata
     9961 tttttttaca catttatatg caaagttaaa tttgtctcca tagaatttac gtattttttt
    10021 aatgtatttt tcaaaacctc cattacttaa gtaatcaaat aaaattcctt ggtctaaaaa
    10081 ggatacatgt atgtttctgc acctttttac actttctagc ttattgatta cttttttatc
    10141 tgcaaatatc catccaattc tcattccagg aaataatatt tttgaaaaac taccaatata
    10201 taagacccca ttattcatat tatctaggga acagatagga aatatatgag aactattgta
    10261 aagtaattct tcattaaatc catcttctat aattgctata ttgtattttt tcataagatt
    10321 atagaatttg tacctatttt caggagtcat tacaattcct gtaggattgt gataagaggg
    10381 tgtaatgtat gcaaacttta ttttattttt atacttaact aagttgtctt ctagcatatt
    10441 gaagtccaaa ccatcttcat ttatatctac acctaagata ttgaggttat gagatttcat
    10501 aatttttata gcagtattgt gagtaggatt ttcacatatt atgtagtctt tttcttgggt
    10561 aaaagatgac ataataatat caaagccttc tgtgaatcca tttgttatta gtatatcttt
    10621 attacttata tcaacaccct tgtttgtcat atattctagt aaatagtcaa taaggggctt
    10681 ataaccttgt gcatatccat agttgagtaa tttatgtcct tctagtgaaa ttctatttaa
    10741 aaaggatttt tttaattctt ccatgtcgaa taaatctcca tctggagaaa tacttttaaa
    10801 ggaaattagg tcagagctcc aaggaatttc actttttata atgtccaatt cattggcttt
    10861 tttagagtat gtattctcta aacaatccca gtttaacgac catgtagtat ttgaagactt
    10921 attctttgat ttaacaaatg taccttttcc tgaaatagag tagattaatc cttctgattt
    10981 taattcttca taagttaaaa ctacagaatt tcggctaact tgtaaaagct gactaagctc
    11041 ccttgtagaa gggagcttac tattgtctgg aattagtcct tttgatatca tgtcacttat
    11101 ataattttta agttgtatat aaattggttc attatcattt aattttagat ttgaaaatat
    11161 caaattacca cctcaaatta tttcatctaa tttaaagagt attctataag tttatctaat
    11221 agttcactgt acttaatacc tctagcagca gcacttctag gaattaaact agtttgagtc
    11281 attccaggaa gagtatttag ttcaagaaca tatggaatac cttcacttat aatcatatca
    11341 actcttacat atgctttgca attaaaaatt ttccaacaag tctcagaaat ttcgtttact
    11401 cttttttgaa gttcttcatc taagtaaaca acttcttcct ttgcaccttt ttcaactgag
    11461 tatttagctt cataatcaaa gaatcctgaa tcagatttta tagatattgt agggaacact
    11521 tctccattta atatgaatga agtatattct ccacctggta tatatttttc aatcattaca
    11581 aattcatcaa cttccagacc ttttctaact gcttcttcaa cttcatcctt agaatgaata
    11641 aagaatgtgg ctacacttga acctcctgag tttggtttta taaatactgg ataacctata
    11701 ttatctattt catcatagtc tatttcatca actgatttta ctaaaaccca tggagcagta
    11761 ggtagatttg aatctctaag cattttttta gttatatttt tatccataca catcccacta
    11821 caaagaggtc cacatccaga gtatggtata tccatagtct caagtattga ttgtatacat
    11881 ccatcttcac caaattttcc atgtaaagca agtattgcaa aatctatatc ttctggtatc
    11941 tttgtaaata tatctttttt atcatctatt attaccttta caacttcgta tttattttta
    12001 tcaaggttgt tatatatctc ttttcctgaa tttaaagaaa cttctctctc tgatgatatt
    12061 ccacccatta taactgctat tttcatatta tacactccta ttttgatttt tattatttta
    12121 aagtaaactt tttatttaca tacttaatta tatgaattaa cagttgagga tgaaagtacc
    12181 actatgtacg aagtttatat tatgtgtggt tttagagtaa taatctagtt taaagatatg
    12241 tttaaatgat tttttaactt tatttatgtt attatttttg ctaaaattat tgttatgaac
    12301 ttaagactag tcttaatggc tataaatcaa ggagtgataa attttgaaag ctgaaataat
    12361 aagtgtagga acagaaatat tattgggaga tatagtaaat acaaattctc aatttttagc
    12421 taaagaactt gcatcattgg gaatagaagt ataccatcag agcactgttg gagataacaa
    12481 acaaaggctt ttagaatgtt ttgatgaaag cttaaagaga agtgattttg taattactac
    12541 aggtggtctt gggccaactg gtgatgatat gacaaaagaa actgctgcag aatactttgg
    12601 tcagaaatta gaactacata aaccttcact agaggttttg gaatctttct ttgttaaaac
    12661 aggaaaaaaa atggccgaaa ataatatgaa acaagtgtac tttccaaaag atgctatagt
    12721 actaaaaaat aataatggta cagctccagg ggcaatttta aaaaaaaatg gtaaatttat
    12781 aatagtattg cctggacctc caagagaaat gaaagctatg tttaatgaga gtgtcaaacc
    12841 ttacttacaa caattcacaa atgaaatgct agtatctaag actttaagat tatatggaat
    12901 aggtgagtca aatctagaat tagaaattct cgatataatt aatgagcaga ctaatccaac
    12961 agttgcttta tatgcaaaag aattagaagt tacaataaga ataactgcaa aggctgaaaa
    13021 tgaaagagaa gcttttaaat taataaaacc agtagaagaa aaaataaaaa ttagagttgg
    13081 caaatatgta tatactgaag gagatatttc catatctgaa ggggagactg ctttagaaga
    13141 tgctgtttct aagctacttg tagaaaaaaa tctaacaata gcagtggctg aatcttgtac
    13201 tggaggtttg gtttcttcat ctcttataaa ctatccaggt atatcttctg tatttcttga
    13261 aggttgtgta acatattcta atgattctaa aatgaaaaga ttaggagtaa aaagagaaac
    13321 tttggaagag tttggagcag taagtgagca aacagctata gaaatggctg agggcgttgc
    13381 taaaggactt aaagctaata taggtatatc tacaactgga gtagctggac ctggtggagg
    13441 aaccaaagag aagcctgtgg gactagttta tactgcaata tatataaatg gaaaaacaat
    13501 agtaaagaaa aatatattta atggggatag aagaaagata agattaagag ctactagaga
    13561 tttgcttaat gaattgagaa tacaattaga aaagctttaa gttgttatga tattggatat
    13621 atttgaagtg ttactataat ataaaattgt ttttggaggc ttttaattta tgataaaaca
    13681 tatattttgt gatttagatg gaactctata tgaaaatgga accataacaa aagaagatat
    13741 aatagcaatt gaagaaatag aaaaaaaggg agtacagttt aatgttgcta caggtagaat
    13801 ttttaaacaa gcacgtaata ttatcaaaga tagtttagat atgaatggat attatgtatg
    13861 tgaaaatggt tcatttatat atgataaaga ttataatgtg atttttaaaa gaacgataga
    13921 tgataattta gtaaaaaaag tcattgatag atttgagtca agtgatgcac aactttattt
    13981 taaatataag ggtgatgtaa ttgtgggtag tgataccact gcttttagac attattctag
    14041 tgactttata gttgacccag attttgaaaa aagaagtagt tttgataatc ttataggaaa
    14101 tataggcgta tgttctgaaa atttggaaga gttatccaga atagaacttt atctaaaaag
    14161 tgagtttagt gaagtattag aaatatattt ttctggaaca tatactttaa atatagttcc
    14221 taaaagtgtg tcaaaaagag gaagtataga gcatgtaata aaaacgttaa atgtaagtcc
    14281 agatgaagtt gctactattg gggattctcc aaatgatatt tgcatgttag aaggatttaa
    14341 atatagtttt gcaatgtcta aggcaagggg agatgtaaag caaagtgcaa attatgtagt
    14401 agatagtgta aaaagtgcca tagatgttat aatggagata aatagttagc ataaaaaatg
    14461 actgtcctag agaagtataa aattatattc tatagagcag tcattttaat tatgttcaat
    14521 tataagttta aaaattctta agttaaccac aatttgctat attatatgat tttattgtgt
    14581 tagataaaat agagtcaagt atataattta tgacactatt acaagtataa gctgaaaatt
    14641 ttaaatcatt catataatca gttctctttt tgtaatcact ataaagttta gtaaaaaact
    14701 tctcaaagct actatttata gtaccacatt taattttaga taaatcaaca tcttttgcaa
    14761 atgctccaag ttctctttca taagtaacat gtttatttaa gctatgctta aattcccaac
    14821 gttcactgtg agctacacag aaaaaatcac ataaaaaatg acatatgact cctagttcct
    14881 gactaagtct acttactgaa aagtatttag aaagactatc tatattaaga ctgcatagat
    14941 agtttatttt agaaacaatc atattaaatg actcatctaa ataatgtttt tttaatttat
    15001 atttagaaaa agcatcaggc tttatattgc catatataaa atttttttcg cttataaaga
    15061 atgttttatt ttcatctata ttatcaagta ttgattttgc cataataaaa tgggtattca
    15121 ttaacatatt tacacctcgt atagtttatt agacttatta aatatattat tatgatatga
    15181 tatttatgat aaaagtttaa tggtaaatat atgaaactaa gtcaatagta tttatttaaa
    15241 taatttcaac catattcata ataccagtaa atggccaaat ttacaatatg taaatagtgt
    15301 taagttttta tttatcaaat ttaaaattaa gtattagtat actttctaat taacctgttg
    15361 gctttagact gtgatatttc aaggtgtttg gctactttat aagaactacc aaatttttca
    15421 taagcatcta aaattatatt tttttcgtac tctttaatta aagtgtcaaa atttttattt
    15481 ttataattag tagattttga gtcagatata ataaccttat taatttggga tatatgtgtt
    15541 ttatttccat tattggtaat atgtatattt tttgtaagaa tgtttggtaa gtcatctatt
    15601 gttatatgat tatctgtaca agtaagcaag attttttgaa ttatattttg taattctctt
    15661 atattgcctg ggaatccata atttgcaata gcatccatag cttctttaga aatagtttta
    15721 tttaggttga agtcacttga gtatctatta aaataatatt ttataatttc cactagatta
    15781 tcccttcttt ctctaagagg aggcaatttt atttctataa catttaatct atagtataaa
    15841 tcttctctaa actttttttc ttttactttt gaaactaaat cagcatttgt agcagatata
    15901 atccttatat caaccttttt attttttaag ctcccaactg gagtaaaagt tctatcctgt
    15961 attaattgca aaaattttgc ctgtagattt tgtggtatat ctcctatttc atctaaaaaa
    16021 agagttcctc cattagctaa ttctacgagc ccaactttac ctttagtact tgcacctgta
    16081 aaagcacctg aagtatatcc aaaaagttca gattcaagaa gttgtggaga tatagttgca
    16141 caatttattg ttataaatgg gccattagat ctattgctgt tgtggtgtat atatctagca
    16201 agtgagcttt ttcctgttcc agattcaccc aataaaagta cacttacatc cgttttagaa
    16261 acatgattta ctgtgtttat gattggtctc atcactattc cgtccataat tacagtatct
    16321 tcaattctaa gtgcagttct gtatagtgtt tctacctcag atttatattt ttttacttgc
    16381 ttttttgtgt cttcaagttt attttttata ttattaagtt cagttatatc tctacaattt
    16441 tcaacagtaa attctaagtt tccattttca tcaaatatag gggtagcagt tatgataagc
    16501 tttttccctg cttgagtatc ttgttccagt gaaaatttag tctttgtttt taatacaatt
    16561 ggtatggggc tttttgttga atatccagag tcttctaaaa aagaaacatt ttttcctaaa
    16621 atatcttctt tttttagacc ataattttct tcaaatgctt tattacaata tattattatt
    16681 cctttatcat cagaaacaca tatttcatca tgggaagctt ctaatatttt ttgataaaat
    16741 tccaaatcta attccaaatt taaattcatt tttatatttt cctcctaaat ttttaacgat
    16801 atattaaaat aggtttaatt gattaatata cttaaaattt gatatttata taagtttcag
    16861 tctattttaa ctttaaataa gttatatata actcatttta caattaaaat aagtctaaat
    16921 caacttaaag taagtgaaaa ataaatataa aaaaattgaa aatttattag tatcgttgga
    16981 aatactaggg ttttagagtc gatatatatt ggtttaagat ggcatagttt ttgcttaaaa
    17041 agaagagtgt aaattgacta aataaaaata gaagggaatt taaaatggga gaaaaaagta
    17101 aagggtatgt atttatagca atagcagggt tattatgggc tacacttgga ttatttggaa
    17161 agtttttaat gggaaatgga ttgacatcag aacaagtagc atttacaaga ttgttttttg
    17221 gatttatagt tttaggcgta tattcatcta taagaacacc acaaatctta aagataagta
    17281 aaaaaggaat tatatattca gtaataatag gtattatatg tcaggcaatg tttaatttat
    17341 gttattttaa agcaattgac attgcgggtg tatcaattgc agcagtttta ctatatacat
    17401 caccactatt tttagcaata ttttcaaaag tatgttataa agaaaatatc actagaagca
    17461 aattattttc acttattctt tgttttattg gtgcaattat ggcagtgagt ggtggacgtt
    17521 tggattttca aggtttaaac gcttttggac tttttcttgg gacattatca gcaatagcat
    17581 atgcacttat gccaactatt agtaaaaatg ctttaaagga attttcaagt tcaacaatat
    17641 tagtatacag tttcttattt ggagctatat ttatgattcc ttcttcaaga ccttgggaaa
    17701 tcttaaatta tgctaaggat ttagatgttt tatcttgtat gctaatgtta ggcattgttc
    17761 cagctgcact tgcatatatt ttttatgcaa caggtatatc taaaggtgta gagttgtctg
    17821 ttgcaggtgt tgtggcatca gtagagttag ttggttcagt aataattggt tgtactatat
    17881 taggagaaag tttttcctta gggaagttat ttggagtgat gttgatgtta atatctgcag
    17941 tagtagcact taatctttct tatgatgaaa taagaatatt ttataagtcc aacaagttaa
    18001 agcagattga aaaaactgaa agtatttaga attatatatt taaaaaggat ttattggtat
    18061 acattgtaga gtccaacaaa aattatacca aattatattt ggtgttagaa aataactatt
    18121 tataaatttt gtagaataaa gtggacagta caaataaaat ataagtagat acagtttaga
    18181 ttatatattt tttattatat attgtataaa tatacaaatt aatattttgt tttgtaaacg
    18241 ctaaataact atagaaagga ttatatttat ataacccttt ctatagttat tattttgtat
    18301 taaacaaact tacagctctg ataagaattt ctagccttta aagtatcatc aatttgattt
    18361 aaaatttccc atttttttag gctccatata ggacctacta gttcatccct cttaccatca
    18421 ccagtgagtc tatggattat catagtttca ggaagtattt ctaattggtc acagacaaga
    18481 ttgatatatt catcttgttc cataagagtt aacataccat tttgtaacat cttttccata
    18541 ggagtatctt ttataacatg caatagatgg atttttatac cgtctacacc cattttacca
    18601 actgcctttg ctgtttccat catcatatta taatcttcac caggaagacc atttataatg
    18661 tgtacgacaa cctttatatt tttagacttt aatttttcaa caccttcaac aaatgtttta
    18721 taatcatgac ctctatttat aattttagat gtagaatcat gaattgtttg aagtccaagt
    18781 tcaacccata aattggttct tttatttagt tcagataagt attctacaac atcatctggt
    18841 aaacagtcag gtcttgtgga tattgaaaga cctatgacat cttcaagttc taatatagtc
    18901 tcatattttt cttttaaaac ttcaagagga gcatacgtat ttgtgtaggc ttgaaagtag
    18961 ccaatatact ttgcatgagg ccattttttt aacatcattt cttttatatt ataaaattga
    19021 ctgataagat tatctttagg gtttccagca aaatcacctg agccttcttt gctacagtag
    19081 gtacatccgc cataaccaag acttccatca atgtttgggc aggtaaatcc tgcatttata
    19141 gataccttaa aaactttttc tccaaaagta tttctaagat agtaattcca agtatgatat
    19201 cttttattat taaaagcata tttaaattta tccaaaaaaa tcacctattt ctaataaaat
    19261 tgtacgctga gcttgtacaa aaaacatatt accacaaata gataacttaa acaaattcta
    19321 ttttaagtaa tataaaaata cagcgagaaa ataattaata ttttctcgct gtatttccag
    19381 agaaaaatag aactagtcca aataatatta tgaaaaaaga cattatcgat actataaatc
    19441 tagataaaaa aagtggagat aatataaggc atataccaaa tagaatctta actatattcc
    19501 aagttgtaaa tttagaataa ggattattct tactatttac aagtgctact agttgattaa
    19561 agattaaata aaatcctaaa aataatgaaa atatgctttg tattttttga ggaaatacaa
    19621 aaagtaatat acatgcaatt attagaaccc attggatatt aatagagggt ttaatttctt
    19681 ctttagtagc atatcttttt aattcattaa tattttttaa atttaaatat gcaaagaata
    19741 aaagtgcaat accaaaagtc caagaaatta attttatacc taatgtctta aatgtaaggc
    19801 ataataatcc taatataatg agtaatactc ccattaacat aaatttagta gcattttctt
    19861 ttttattaaa gttattaaaa tcgatattaa aatttgtgta cataaaatca cctccaaaat
    19921 cgatagaaca attataacag aaattcatat ccaaataaag cctagtgtat aaaaattaaa
    19981 ctatacttag taattaatta ataggttgta tcataaactt ctattaatag aattatatag
    20041 aaggaggttt gtccatgaca aaattatggg caataatatt tgcattatta gcaggtggtt
    20101 ctacagctct tgaagctttt ataaatggag aactaggaaa aagtacaaca gcattagtgg
    20161 ccacctttat aagtttaact gtaggagcaa tatttttttt ggcaagtata atagttacag
    20221 gtgacttaaa gtgtctagta tctataaatg gttttaatta taagttgtta ttaggaggag
    20281 tatttggagg tctaataata tattttacag taaaggcaat tccaaattta ggagtatcaa
    20341 atacacttat attaacactt gtatcccaag ttttagttgg attttttatt gatagcgtaa
    20401 ttttgggaca agaaacaatg catttataca aatacatagg ggttatactt ttaatattag
    20461 ggacattctt tatagtgaat taacaattta atataatttg ctatgcaact caaaaatttg
    20521 gtataattat gttaataaaa caaagttaga tgaaagaggt gttaaaattg gctgatgaaa
    20581 aatttgtaat gtcatttagt ggtggtaagg atagtatatt agcattaaat cgaatgttga
    20641 aaaaaggata taagcctgtg gcactcttga caactataag tgaagaacat ggaaagtcat
    20701 ggacacataa tttagaatat aatatgctta agcaagtaag tagcaatata ggattacctt
    20761 tattagttgc tgagtgtggt gtagaaggat atgaagaaag ctttgagagg gctttaataa
    20821 aagctaaaaa catgggagca acaatatgcg catatggaga tatagatata gaaagtcata
    20881 gaaaatggga tactgataga tgtgaagcag ttggtatgaa agtagattta ccattatggc
    20941 aagagagtag agaggattta gtatatgaat ttatagacag tggattttgt agtgttgtaa
    21001 caaaagtaaa cttaaaacac ttaggagagg aatttttagg aaaaaaatta actagagaac
    21061 ttgtggagaa gataaaaaat gctggtgcag acccatgtgg agaacatgga gagtatcata
    21121 catttgtcgt agatggtcca atatttaaaa aacctgtaga atatgaagta aaaggaactt
    21181 taataaaaga tggctatgga tatttagaaa taaaataatt acataaataa aatacctatt
    21241 atataatcct aatttttgca aaggtttaat atttgcaaga ttgtataata ggtatttttt
    21301 atttagatat tttcatatat tgccactctt atcatctaag caaccttatt cagattagag
    21361 tctaatgtag aaaaattctt ttttagaaaa accaatgata aaataagaca tataaactca
    21421 gaaacaatag tagtcatcca tattccttgt ccaccaaata ttagtatcat tgaaattaga
    21481 cttaaaacaa ctattacaag ccccctacca agagatataa cagtagaaat tgatggtttt
    21541 tctactgata caaagaatcc agaaattatt acattaattc ctaccaataa gaaagatata
    21601 gagaatattt taaatacacg aactccttca ttgaatagca ttgtttcttc tgggtgtata
    21661 aataaagaga ctattggttc tgaaaaagcc atacaaattg caaatacagc aattgatgct
    21721 attattgtgg cttttatccc catttttaat aattttttta cattatctat atttcctgca
    21781 ccataataaa agctagtaag tggttgcata ccttgagtta tacccatcat agtcataagt
    21841 actaatgtat ttatataatt tatgacacta taataaataa gagcattttc tccaatatat
    21901 ttagtaagac ttaagttaaa aagtaaaaca actataccac aacttaattc agtagttgaa
    21961 tcaggaaaac ctataaatac aatttttcta agtgtcttaa aatcaattct aaattttgaa
    22021 aaattcaaag tagagttttt tcttaaaaaa tgtattaaga agaaaatagt agaaaagact
    22081 tgagacaaac cagttgcaat accagcacct tttacacccc atccaaattc aattacaaac
    22141 aagtaatcaa gtatgatgtt tgtaagagca gatattatta caccaacagt agctaatatt
    22201 ggaaagccat cagtttttat tataacttct aaagaataag acactataaa gaatccgtta
    22261 aaaaatatta ttattcctaa ataatcttta accataccaa ttgtagattc tgtagctcct
    22321 aaaaatagtg ctaatctatc taaattaaaa aaagttatag ctaatataat tattgataat
    22381 ataattattg atactaaatt aaatgaaaag acttcgttag cagatttaat atcttttttc
    22441 cccagataaa ttgcaattat agtagatgca ccagttgaaa ataatagaga aaccgcaaat
    22501 ataaaattaa taaaaggcat tgatatattg acagcagcta aagctaattc tccaactcct
    22561 ttacttacaa atattccatc aaccattgta tatagtgaga aaacccacat tgcaactaca
    22621 gatggtgcta ggtatttaaa aaatctttta cctagactca tgttttcgtt tatagatatt
    22681 tttgtatcca tttattatca ctccctgtaa atctattata aaattacgaa actattaatt
    22741 aataatttta tttctaatat aaatgataaa ctatggtata ataccaatgt caacagcaaa
    22801 tagtatataa ttataaattt tattaaataa tagtgatttt aagggtctta taaatatatc
    22861 ttatttatat attactgaca tttaactgat aaataggaaa aaggtacttt aagtgacatt
    22921 taattgacat aaaactgaac attatttgaa tagtattttg cttatatttt cagatgtttc
    22981 tttatccatt tcatctaata agtgagaata tcgaatatct attcatagct atttttatat
    23041 ctgcatgtac taatatttta taaatagctt ttatgttagt acctgaaagt aatatcaaag
    23101 ttgcatttgc atgtattaat tcatgtactt ttatatctat aggattatta tatcttaata
    23161 gttttttaaa atgaacaaca aaagttggtt gaactcacgg gttgaatctt ttatttaagc
    23221 atatcaaatc aaattcattt ttcaatatag cctgtaattt taacttattt tgtttaaatt
    23281 tttcctcttc aagtaaactt ataagttcat caggagcaaa taattttctt ttggatttag
    23341 gggttttagg gtctttgaag attattttat tgtctacata aactaatgtt tgatttacat
    23401 gtatgatttt tttatcaagg tctacatctg accatataag tgatacagct tcaccaaaac
    23461 gtaatccaag agttaacatc aaaaaaattg gtatacctat aggaatattt tttgattttt
    23521 ctaataataa ttgagctttc tgttttgtat aaaacttaga agttgaacta ttttttttga
    23581 ttagggttgt aacaaaatca caaatatttt ctgatatttc ttttaatcta taagattctt
    23641 tcaatactga ttttataaaa ccgacttggt tttttgctga atttaacatg tgctcattgt
    23701 atatgttatt tatataagac tgtaaaatag aaggcgttat atctattaat ttcatatgtt
    23761 attggaaaat cgaataataa taacttggta ttcgaaactt ttgataaagt aatagaacta
    23821 tatccaaatg caaaacctgt ttttcatagt gatagaggtt ttcagtatac tagtaaggtg
    23881 tttgaatcga aacttttagc acaaggtatg atacaaagta tgtcaagagc tggtcgttgt
    23941 attgacaatg ctcctatgga aggaatttgg ggaattttaa aggtggaaat gtactattta
    24001 aggaaatttg atacatatga acaattagtt aaaataatag agaattatat atattactat
    24061 aataatttta gatatcaaaa gagattaaat tctatgtctc cacttgaatt taggaaatat
    24121 ttaagtacag agatagcata atttatttta agtagctcta aaaaaaatta aatatctagg
    24181 gttttatttg acgtgctctt ttttagatag taaactttaa aatatagata ttaattatat
    24241 gaatataata aaaaaagtac taatgagtta cactagtact ttataactac ttttacatgt
    24301 tttaactgta taaaacagtg ggtatagttc aggcaggagt gactttagtt ttgaactaaa
    24361 aatcaagttc aaaaagaata aacattagta tttgaacttc actttacatc taaatagatt
    24421 gtagttcttc ttgtttttat tataccacaa attggtacag atattcaaaa ataatatatt
    24481 tatgatataa taaaaatgta aatagttttg cagtgagcga tatttgttac aaagtagggc
    24541 ttaacgcttg aaatataagg tgttgagggt atatgataaa ctttatcatt tgcactactc
    24601 atggttcact gcaaatttaa gagagttgca catgtgtaag tattgaaaat gcccagttta
    24661 ttttggggtt ttatattaac taagtggtat gtaaataaag ttaaatttat tacatctaat
    24721 tctgtctctt catgttttat attaactaag tggtatgtaa atacaacagc accataaaaa
    24781 aaaccattcc taaaaaactg ttttatatta actaagtggt atgtaaatag tataatgttg
    24841 aaaagttaga gagtacaatc aagaagtttt acattaacta aaatgtatag aataaaacat
    24901 gtatttatac ttaaattctg tacctatata aaaaagtgaa ctctgtcaac aaagcacttt
    24961 tttatataga tgaattatca ttttgtttta agatagaaca tactaatgat aaatgtttat
    25021 cattagtatc tgtatgtaca taaaagttta attttttata taaatttgct ctttagaaaa
    25081 atgggcagta tcaataaata tattgtctaa atttttttta ggaactagtt gactagccat
    25141 aagattagct tcaactcttt gattgttaga ctatgaaatt aaatttaaag gttcattctt
    25201 agtcatataa atagctttat tattcgtatg tactataaca atttttgcca tctgcttttg
    25261 atagataaag agctttatca gctttagaaa ataaatcttt atataattta gttgaatcat
    25321 cagtgaaggc aataccaatg cttaatgtta ttttatgatt gtcctttact tttattttac
    25381 ttgcatcatt taaaatatca ttagtaattt ttattattaa atcctttgtt aatgatttta
    25441 taaatattgt aaattcatct ccaccaatgc gagcaactat gtctttatca tgtgtgttat
    25501 gtaatagtat ttttgataca gcaactaaaa cttcatttcc ctctaaatgt ccaaaagtat
    25561 catttacagt tttaaaatca tcaatatcaa taactaatag agcatcatta tttttggaat
    25621 cagaactaca taaatactct tgaataagat tttgtgatac tctttgatta tacaattgtg
    25681 ttagtgggtc acgttgagcc tgattaatta agctttctaa tttagattta tcttcagtta
    25741 tgtcttttaa aacgcctatt gttttgatgg ggttatggtc actatcatat ataactgaac
    25801 attgaacacg aatccatctc cattcatttt tagttaatga atttatttgt cttatctcaa
    25861 ttgtttcacg aagatttcca tgaagtactt ctagtagttt tccaatatca tcaaggtgaa
    25921 taattttacc aaattctaat agtttggagt aattttttgt ttcaaaattt tctaattcaa
    25981 tttttttatc aatttctaca cgttgatatt ttataagggt atcgttttca atatcatact
    26041 caaacatgat atctgtaata ttttgcaaag caatacgata tcgctctctt tctaatttta
    26101 gttcagattc tatgtttttt agttgggtga tgtcagtaat aatactatta atttttacta
    26161 tgccatcact atttaacgat ttcattccgg aatctaatac ccacattaat gttccatctt
    26221 tttttcggat tctatattca gatgaatatg taggtccttt tgcaaaacat cgttcacaat
    26281 cttctagtgc tttaggtaag tctggtggat atacagcacc cactgctgaa ccaccactca
    26341 tttccatgaa ctcatcatat gtgtatccta atattttggg caatccttca ttgacataga
    26401 aaaatgagta tgtactatca tcattgctac cttttaagcc accattgata ttatttgtaa
    26461 tcatttcaat aacttctgtt ttttccttta gtaatctttg caaataatca taactttgag
    26521 ttccaatttt agtgggaaag tattcatcaa gttgcatttc accataaggg tttgatatgt
    26581 gcatgtgctt tactaataat tttcctctat cctcaattat ctcaaatgta caacgttgat
    26641 ttacctctaa aatcatttga gcattttctt tagttcgaac agtatatttt cctaatacca
    26701 tacatctatt ttcatcttca cttacagtct caaatatatc attatttata tcacaagaag
    26761 gaatttcatc ttgacctgca taaaaataat tttttatttc attaaaagaa gtgagaaatt
    26821 cctgttctcc tggtccaatc catgtaacat tttcacaaag gtattttaat acttcatcaa
    26881 tatctcgatt tatataaaaa cttgttataa tatgttttgc taatttgggt gcttttttta
    26941 ggagtatgct catgatattc ctcctttctt tttgctatta tattttaatt atattataca
    27001 tcaatatatt taaattaaaa gctttgttaa actatgatat ctttgttaaa taagttaatt
    27061 ttaaaaaaag aactgaaaca atatatgttt cagtacttaa tgttaatatc ttgtatagat
    27121 acatatttat aaactaatgt acaagttaaa aaaagaaaga taattttaat ttattttaaa
    27181 ataacaatgt taataatcat ttttatcaaa ttttttcttg tttttctctt tgtttttata
    27241 cattttttta tctaattcaa ctattgtcaa ttttaaattt ctttttactg tatcaacacc
    27301 atagctaaat gaaagatatt tatacttaga gaatactttt aaaaaatttt ctcttaaggt
    27361 ttctagttgt gaataaacat aatttttgtc aacatcacta gagaatataa caataaattc
    27421 atctcctcct aatcttataa tagaggtaga atttattaat tctttattta aaagtgaaac
    27481 aaaatcacat aaaatcttat ctcctacatt atgaccttca tagtcattta tcattttgaa
    27541 attatctaag tcaattagta caatataatc tcctataaat atgtcaaatc tatcatttaa
    27601 atattttcta ttatatgctc cagttaatgg gtcagtaatt gagtagtgaa gatttctctt
    27661 attttcttca tagagttttg ttatatcatt ggccatagta attatgtagg gtttttcatc
    27721 aacatctact ttgtattcta aaacattcca ataacttgta gtatcttttt taaactctaa
    27781 aatccaattt gaaattttat cataaggata atttttttcc caatctaatt gaatattgtg
    27841 agaatccgta aaaaatttag caatattatc agggagtttc ttagctattt ctatattagt
    27901 cattccaagt ggattatctt gcatataact tttaattaaa ttttttaaat tatcatttat
    27961 aaatataatt ctgtagttag aatctcttat aattagaaat cctggaaaag ttcttaaaaa
    28021 aatttcttta aacatattaa tttctccaaa ataaaatact ttgtactaga tttttgttaa
    28081 attttttatt ttatttatat ctatatacat accttgggca atcgaccttc catcaattgc
    28141 agaaaggtta cattgtgtat acataactgt gacaatttga ccatctcgtt ttatgaatct
    28201 atataaaaat ggttcaattt tatttgtgtc aatcatatcc aaactttttt tataaacaaa
    28261 gtctaaatca tctggatgaa caattgctct taaatttccg cccatctcac caaaaaactg
    28321 ttcttttgta taacctataa tatcaaacat ttcttcattt atataagtaa taatcatatc
    28381 accaaataaa tcatagcgaa ttatacccac aggcaacatt gaataaaatt gtgataaaac
    28441 attactttca tcaagatgtg tatttgtcat tttaggtatc gaatatgatt caaattcatt
    28501 tatatagcca gcagttttaa ttaaatccaa atgtaatttt gctaagagta atttattttc
    28561 tgaaattata caatttgcac taaagtagaa attactatat ttttgatgtt tagcagcttc
    28621 atctgataaa acagcttctc cactaattaa taatgaatca gattcagtat tatgtggggt
    28681 tatacttttg taaagaatag aaaatttttt acctttaata tgtttttcaa aatgttctat
    28741 tactttagga aaagatgtaa tttcagtttt gttgtaaaaa tcaaaccatt gaatgtcttc
    28801 agtaacatta gctcttagtt ttttcatata ttgcataata aaaaatccat caaatatctt
    28861 ctctattaaa gattttccta tatcagaata ttttataaac attttctctg aattattctc
    28921 tttagatagt aatttttcaa attctttgca ctccataggt tttgcataat aataaccttg
    28981 ggcgatatca caatttatac ttttaataaa ttctacatgt tgctctgttt caacaccttc
    29041 acatactgta agcatcccaa gagctcttgc aatgtttatt atagagtaca aaatggttgt
    29101 atttttaaaa tcatttaaat tatgagataa aaatatccta tcaattttga ttatatcaaa
    29161 aggtagattt tgcaacatac caaaagatga atacccagaa ccaaaatcgt ccattgatac
    29221 agaaaaacca taagaacgaa gttcagttaa ttgttgaata attgtatttg tattttctat
    29281 taatatattt tctgttattt caatgctgaa atttgaggta gagacgttgt acttttcagt
    29341 aatacctttt agcatacttg gaaaattagg attaaaaaaa tgaagacgag aaaagttaca
    29401 acaaattgga acaaaaacat catttttatc aatccatttt cttatatttt tacagttctc
    29461 ttcaaataaa tgcaaatcta attccgttat cagaccaaac tgttcaaata aaggaataaa
    29521 tttatctgga gtaagaagtc ctttaatagg atgtctccat cgtgcaagcg cctcacatcc
    29581 aactatagta ttgtttttta tattataaag tggttggtaa aatacttcaa tttgtttagt
    29641 ttgtagaaca tatgatattt cggataagaa ctccttatca ttttctaatt tattgtaaaa
    29701 gctgttatct ttaaaattaa atagaatgta tctatttttg ccttcacttt tagccttttt
    29761 taaagcacta tatacttggt tgaaaagcat caaagtagtc atgtttggtt ccccattcca
    29821 gatagtgctg ccgatactaa atgtaacatt ggagtatatt tctttttcat taggaagtct
    29881 tatttttaat tctcgagcac tctctataat agttgcaccc atattattta actctttatt
    29941 tgatacattt ggaagtacaa ttaaaaaacc ttctcctttt aaataagcga ctattccctt
    30001 tgattctgga ataaagtgca ataggttttg agttagagta attaataaat tttctccgac
    30061 tagatatcca tatatatcat taaataattt aaagttatcg atatttaata acatgattcc
    30121 tatatgtata tttttcgaaa tacagtcatc aatatattgg tacataaatt ctctgtttgg
    30181 tattttcgaa aaaaaatcta tttcatttaa ggtttttaat ttaaaatttc tactatctaa
    30241 ttccttagat aatacttcta cctttttatt taacattttt atataattat gattttgcat
    30301 ggtattcctc cataagatac tttaaatttt gatgacaact gtatttatgc tagaagtatt
    30361 taaatgttaa tttgtaaata atttaaatgc ta
//
LOCUS       DAEMAE010000017        30041 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEMAE010000017 DAEMAE010000000
VERSION     DAEMAE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.017"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..473
                     /locus_tag="KPH93_000682"
     CDS             57..473
                     /locus_tag="KPH93_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687519.1"
                     /translation="MYKEKLFDNYFKFLALLFWPIMWYKWIVISNGTLENMLFTIYAI
                     IAIVFIILYSVFMIKYKDITQIDFFYRISTLLAFIFTLFSFLIYPKSLFFLYLKIIFT
                     GIYLYYSIVKTLKFKDDEGVVGIMSSLLLIVITLFY"
     gene            594..1349
                     /locus_tag="KPH93_000683"
     CDS             594..1349
                     /locus_tag="KPH93_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1687520.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLENLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(1492..1920)
                     /locus_tag="KPH93_000684"
     CDS             complement(1492..1920)
                     /locus_tag="KPH93_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG1687521.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(2341..2541)
                     /locus_tag="KPH93_000685"
     CDS             complement(2341..2541)
                     /locus_tag="KPH93_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1687522.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(2724..3893)
                     /locus_tag="KPH93_000686"
     CDS             complement(2724..3893)
                     /locus_tag="KPH93_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG1687523.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     KLQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            4129..4950
                     /locus_tag="KPH93_000687"
     CDS             4129..4950
                     /locus_tag="KPH93_000687"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG1687524.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(5026..6414)
                     /locus_tag="KPH93_000688"
     CDS             complement(5026..6414)
                     /locus_tag="KPH93_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG1687525.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLILILLFSWL
                     LSSGVILPANYAEKQINRYKEKIISSEKVTEDIIPNIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPVLRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYIEYISKGVTQIEKYINTLIDISKTENGYILNKEVINMSEFIKDILVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKCLKFVL
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(6549..7307)
                     /locus_tag="KPH93_000689"
     CDS             complement(6549..7307)
                     /locus_tag="KPH93_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG1687526.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYVI
                     LVCLGFVATLVSVLGFKFGISEAYFTSNFYMISILILIGCSLFEYILHLFLSLRFGKG
                     ASIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(7309..8025)
                     /locus_tag="KPH93_000690"
     CDS             complement(7309..8025)
                     /locus_tag="KPH93_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG1687527.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILFCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISISQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYIFPYSIPSRLMAVVLHILPNGLPVPEGSELLNNAVILPGVLISI
                     VLFVLFALATRVWFNSREVK"
     gene            complement(8027..8734)
                     /locus_tag="KPH93_000691"
     CDS             complement(8027..8734)
                     /locus_tag="KPH93_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG1687528.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKPNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWSRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLEIVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVKQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGE"
     gene            complement(8850..9386)
                     /locus_tag="KPH93_000692"
     CDS             complement(8850..9386)
                     /locus_tag="KPH93_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG1687529.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(9488..10558)
                     /locus_tag="KPH93_000693"
     CDS             complement(9488..10558)
                     /locus_tag="KPH93_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG1687530.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSYLFVYDN
                     VASNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFIDNIEEYIPENVNIKEKIGRNSNYDSEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEVIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(10551..11609)
                     /locus_tag="KPH93_000694"
     CDS             complement(10551..11609)
                     /locus_tag="KPH93_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG1687531.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGIEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIDNKKGEDKHE"
     gene            complement(11596..11928)
                     /locus_tag="KPH93_000695"
     CDS             complement(11596..11928)
                     /locus_tag="KPH93_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1687532.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            12127..13248
                     /locus_tag="KPH93_000696"
     CDS             12127..13248
                     /locus_tag="KPH93_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG1687533.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(13376..15274)
                     /locus_tag="KPH93_000697"
     CDS             complement(13376..15274)
                     /locus_tag="KPH93_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG1687534.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            15589..16365
                     /locus_tag="KPH93_000698"
     CDS             15589..16365
                     /locus_tag="KPH93_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG1687535.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIIPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(16457..17209)
                     /gene="xth"
                     /locus_tag="KPH93_000699"
     CDS             complement(16457..17209)
                     /gene="xth"
                     /locus_tag="KPH93_000699"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG1687536.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADVFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEVLGSDHCPVELEIK"
     gene            complement(17362..18312)
                     /gene="galT"
                     /locus_tag="KPH93_000700"
     CDS             complement(17362..18312)
                     /gene="galT"
                     /locus_tag="KPH93_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG1687537.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAYATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESNKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLNRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEELTKKLKID"
     gene            18697..19893
                     /locus_tag="KPH93_000701"
     CDS             18697..19893
                     /locus_tag="KPH93_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1687538.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALKK"
     gene            complement(19984..21345)
                     /locus_tag="KPH93_000702"
     CDS             complement(19984..21345)
                     /locus_tag="KPH93_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1687539.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKNVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLGMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKALVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     QQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEKESGI
                     SLN"
     gene            complement(21484..22236)
                     /locus_tag="KPH93_000703"
     CDS             complement(21484..22236)
                     /locus_tag="KPH93_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1687540.1"
                     /translation="MNLIDKLDINRKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(22384..23961)
                     /locus_tag="KPH93_000704"
     CDS             complement(22384..23961)
                     /locus_tag="KPH93_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1687541.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFA
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPKTISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHILGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     MLGLVYALVYYNVFKILILRFNLQTPGREEIADINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENALKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(24365..24817)
                     /locus_tag="KPH93_000705"
     CDS             complement(24365..24817)
                     /locus_tag="KPH93_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1687542.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITQE
                     GDKIAADFMDKLSDGLGEKLSKLTEEDRRKYLEAINTLQYILNKEEFR"
     gene            complement(25128..26573)
                     /locus_tag="KPH93_000706"
     CDS             complement(25128..26573)
                     /locus_tag="KPH93_000706"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG1687543.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLINLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESINPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(26717..27625)
                     /locus_tag="KPH93_000707"
     CDS             complement(26717..27625)
                     /locus_tag="KPH93_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1687544.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            27882..28706
                     /locus_tag="KPH93_000708"
     CDS             27882..28706
                     /locus_tag="KPH93_000708"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG1687545.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            28746..29276
                     /gene="lepB"
                     /locus_tag="KPH93_000709"
     CDS             28746..29276
                     /gene="lepB"
                     /locus_tag="KPH93_000709"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG1687546.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(29358..>30041)
                     /locus_tag="KPH93_000710"
     CDS             complement(29358..>30041)
                     /locus_tag="KPH93_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HBG1687547.1"
                     /translation="YNGIEFDDKIILSKNYRQAKEIVDFTNKFSNNSNSYINELRPNT
                     EFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYLISKKGLDYSEVAIVYPYN
                     KKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKKVGTTIANIYAIKNLEYKA
                     VIVCELEMLYNQTINDTEQDYQVNDFVGDLNKVYLAMSRATDYLSIVTTFNEEASDII
                     RLITESKDI"
BASE COUNT        10116 a         4795 c         3469 g        11661 t
ORIGIN      
        1 ttatttaaaa ttctaaaaac aaatatatgc ttttgttaag aaaggttgtt tgatgaatgt
       61 ataaagaaaa gttgtttgat aattatttta agttcttagc actattattt tggccaatta
      121 tgtggtataa gtggatagta atttcaaatg gtactcttga aaatatgctc tttaccatat
      181 atgcaatcat cgcaattgtt ttcatcattt tatattcagt gtttatgata aaatataaag
      241 acattacaca aatagatttc ttttacagga taagtactct acttgctttt atatttacac
      301 tttttagctt tttaatatac ccaaaaagtt tatttttctt gtatttaaag attattttta
      361 ctggaatata cctttattat tcaatagtaa aaacccttaa atttaaagat gatgaaggtg
      421 tagtgggtat tatgagctca cttttattaa ttgtaataac tttgttctac taaatttaat
      481 tctatttgac ttaatatggc tttaaatatt agacatctac attattttct gatattattt
      541 aagatggtag tcttatatta ataatgaaat acactatgaa ggagttaact attatggaaa
      601 aaaaagtttt agcaacagtt ggagaaaaag agataactaa tattgatatt gaaaatgcat
      661 taaaatcatt agacccttat caagctatgc aatttaaaac tgaagaagga aaaaaacact
      721 tattaaatga tttagtaaat caagaattat tcttcttaga tgctaaagaa gaaaaattag
      781 atgaagaaga aatatttaaa ttagaaatga aaaagataga ggaaaatgta ttaaagcaat
      841 ttgctataaa taaagtttta tctagtgtta atgtaacaga agatgaaaaa gtaaaattct
      901 ttgaagctaa taagagctct ttctctaaac cagaaagtgc aactgctaaa catatattag
      961 tagatagtga tgaaaaagct aaagaaatac ttgctcaaat aaaatcagaa gaaatatctt
     1021 ttgaagacgc agctcttaaa cattcttctt gcccatctaa agatatgggt ggtgatttag
     1081 gaacattcgg tagaggacaa atggttcctg aatttgaaga agctgtattc tctatggcta
     1141 aaggtgaagt aagtgagcca gttaaaactc aatttggata ccatataata aaattagaaa
     1201 accttcaaga aagtacagag tctacatttg atgaagttaa ggctgaagtt gaaaaaagtt
     1261 tattatatca aaaacaaaat gaagtttatg gaaataagat aaatgctctt aatgctaagt
     1321 acggaaacct tgtaaaatac aacgattaat cataaggtca tatataaaaa agctgtcttt
     1381 tcacaatatt tattagaaaa gacagctttt tatattaaag tttaaatatc cagtttcata
     1441 ttatcattct tttattgtaa agaactcagc ttaaaagttt aaataagatt tttaattatc
     1501 attagctaaa tttttatatc cacgtattat ctcatagcag taacctatta catctcttct
     1561 ttttattatc ccaataaatg tgtcttggtc atctataaca ggaatgaaat tttgatttaa
     1621 agattttata actaaatctt caatatctgc gttgatggaa acaggagaat tatccatttt
     1681 tctatttata tctgtcactg gaacatcttc caatcccttt aagtccagat ttaaatcgtt
     1741 ttttaatgtc cataaaaaat caccttctgt tatagttcca acatatttac catccttact
     1801 tattattgga attgcagagt atttgtgata ttccattttt tctagggctt gccttatagt
     1861 gtagtcttca tatatatatg ctacttcact ttttggtgtt agaaagaata atatgttcat
     1921 tacactaccc accgttactg aaaacttata aatattattc aatatttata atgttacttt
     1981 tttgcagtaa ctttctcctt tcttattttc taagtattct taatactata cgctgtaaca
     2041 agcgtttttc ctttgctcat cttaatctta ttatgcttta ttaatatttt tatatggtct
     2101 atcagtatca cttattctct ttttagaaaa ttcactaaaa attaattgac ttaaacttag
     2161 ccataataat tttactctac actattaatt ttaacatatt tatctcatta ttaatgattt
     2221 tattttaata attttaattt attttgatat taattttatt tacaaaaact gaaaaaaccg
     2281 ctaaatgatt agcggcttat ctctcatatt actagaacac taatatatat gaatttttaa
     2341 ttataatcta actacgttag cagcttgagg acctttagct ccatctacta cttcgaactc
     2401 aacgctttgt ccttcttcta atgttttgta tccatctcca gttatagctg agaaatgtac
     2461 gaaaacatct ccttcacctt ctacagatat gaatccaaat cctttttcgt tgttaaacca
     2521 tttaactaca ccagttttca ttaataaatc ctcctaaaaa aacacaaata tttagctact
     2581 cttttctgta accttattac tactctatca tatatagcta tgtttgtcaa ttattattat
     2641 ttttatacat taataatcaa tttttttact ttataatttt aaatcgttat tatcttttac
     2701 actttatttt tctttataat tttttaataa tttattttta ttatatggaa tcttatggac
     2761 ttatcactcc agcctaaatc ccatggcacc aacattctat cagtattatg gcaagttacc
     2821 aaaggatatc ctcttgagtc taatcctgtc actgtcgaaa catgtgttat tctgcctttc
     2881 ttctcataac caacaaaatc ccctggacgt aaattataag cttctttgta tacttcttca
     2941 taagaacctt tagcaattaa tgaacctctt ccactataca gtgcataatt tttaaaagct
     3001 tgtgcattta cccatgcctt tgtacctgct ctttcgctat aattccaaat tgagttcttt
     3061 ttgaaccttc cactttcaaa cattatctgt gatgcaaagt ttgcacaatc tccaccttct
     3121 ggattataat ctctatagtt tggattaaat ttcataccat gttcttcatc agctgcggct
     3181 ccacagtatt tatgtgcata atctatagct tttttttgtt catctgtaag ctgtaacttt
     3241 acaccatctt gttggccaat ataatttctt atatcatcag attttatatt ttctaaattt
     3301 aaagagtctg caaaagggtc tgtataccac tctttagtta tgacatattg attatctata
     3361 atttttacat ttagataatg atatgtacct attctaaagg cattttcttt gtccttttga
     3421 ttttcatatg aatacttata ttcagtcgat actgaacaga ttataccata aaggtctttt
     3481 tctttttcct ttattttttt aatcttaacc cttgtcttta tgtctttaaa ttttacacct
     3541 tgcttactag accagttttc taaatacttc atctttttta cttcattttc ataagcccac
     3601 agaccaaatt ttttatccgt ttcataaaga ccttttaaaa tgttttcatt tttctctaat
     3661 attgccttat ttctgtagtc aaataagttt tcaagcaata gtttatattg gtcttttaca
     3721 tcatcacctt caacatttgc agattccatg actttttctt ccatattttt catacttaaa
     3781 tcattgatta aaaatacacc tgctgtagta gctaaagaca aaaatacaaa tgccattata
     3841 cctttgatgt acttattctt atttttcagt attttcaaag ctacacctcc caaatagtac
     3901 accttacaaa tcaaattaca aagaatattc tgtcacatac agtaattttc atatttaata
     3961 attctctatt ttattatgtc caatattaaa aattaattgt atcaatacac tgtttttttt
     4021 acaatttttt attttataat ctactttttt ctttttttta tagtaatata tatgtatata
     4081 aatattttgg tatcattata attaatacat actaatggag ggttttttat gcaaaaaaaa
     4141 gtcgcggcaa ttaatgactt atctggaata ggaaaatgct cattatcggt tgctatacct
     4201 atactttcag cattaaaggt tcaatgttgt ccatttccaa cggccatact ttcaagtcaa
     4261 actggttatc ctgaatttac ttttttagac tttactgatg aaatggtaaa atattcaaat
     4321 gtttggaaaa atttaaaagt caattttgat agtatatata gtggtttttt gggttcaaag
     4381 catcaaattg agatagtggc taattttata aacgattatc ccaatgcttt tatcgtagtt
     4441 gaccctgtga tgggtgataa tggagttatg tatccaatat tcacagaaga aatgagacaa
     4501 gaaattaaag aattagttaa acattcagat ttgactactc ctaacttaac agaagcttgt
     4561 ttcttaactg gaaatgatta tactaaaagt gattataata gagatgaact tatatatatt
     4621 gcaaaatctg tatctgattt aggacctagt aaagtcgtta ttactggaat attagaagac
     4681 gataatatac taaatttggc ttatgataga gataatgacc atgtattctt tacatcagtc
     4741 aaatacaata attgttctta tagtggaact ggagatatat tcacatctat actttgtggc
     4801 atgttagtaa ataaacatga tttgggagta gcggttaata ctgcaacaga ttttatatat
     4861 aaaactataa attacacatc tcaatttgat accgatagaa atgatggagt tatgtttgaa
     4921 aactttttat cggatttaac taatatataa aatcaaaaaa actatccatt ttataaatac
     4981 ctataaatca taaaatttag gtatttacaa aatggatatt atttattaaa ctattggtat
     5041 ttcaattgtt accatagcac caccagtttt cacagaattt tcaatatata aagtcccatt
     5101 atgtttttgt acaatattat ttacaatata aagtcccatt ccataatgag atttagaatt
     5161 tctactcaaa tctcctgtat aaaactgttc tgtagctttt gacaaatctg ctttagaaaa
     5221 accactccca caatctgtaa gtacaaattt taagcattta ttacttactg atacacttat
     5281 gtatagttta ctctggcttg gtgaataatc tatagcattt gaaattatat tcataatagc
     5341 tctgaataac agttctctat ctatagttat atttttaggc aaattatctt gtttataaaa
     5401 ctccacatct aagtttttag tactagctaa tgcttctatt tgcactaaga tatcttttat
     5461 aaattcactc atattaatta cttctttatt taatatatag ccattctctg tttttgatat
     5521 atctattaaa gtattaatat atttttctat ttgagtgaca ccctttgaga tatattctat
     5581 gtattctaat aactctgggt tttcatttat ttcaattaat aagtcagtat ttccatgtat
     5641 aattgttaat ggtgttttta tatcatgtgc cagtgcagat atctgttcct ttctattttc
     5701 ttccaacatc cattgttttt ctaaagaatc ttttaacgcc aacttcattt tatccataga
     5761 aaaaagaaca ttattaattt cataaatttt actagattct acaacaaaat ctaaatcctg
     5821 ttgttctatt ttttctgtag tattttttaa aagctccatt tcaatattaa atttcttgcc
     5881 aaatactttt gataatataa caatttcaat aaaaaatatt atactaaaaa taatcatacc
     5941 caacgtctct ggctttggta aatatgctct taaaactgga gaactatatt ctgatactat
     6001 tttgtatttt attataaaaa cttcatcttt tttaaaaaac tttatataac tttccgaata
     6061 tgcatgcctt tcttcaatat ctctcattaa attccatact tcttttgatt cttttttatt
     6121 aaaagtacct gaaattaaat tcccatctaa agtataaacc ccatactcgt atatatttgg
     6181 aattatatct tcagttactt tttcacttga aataatcttt tctttatatc tattaatttg
     6241 tttttctgca taattagctg gtaatattac acctgatgat aatagccaag aaaacaataa
     6301 tatcaaaata agtacaattg aaattgtcat aataaagaat aaagataaat attttataaa
     6361 aatagttctt aacttttcaa cttttttgtt gttagatttt ttacctccaa gcatttgtac
     6421 catgtcctcc aatagtttaa ttaattcata attaatacct aaccagtttt ttatttgatt
     6481 attttatatc tttttattgc taagttatat tttaacataa taatataaat aaacagtcaa
     6541 tttttattct attcttctga ttttttacct tcccatttag aaaaccaaat acataaaatt
     6601 acaaatgcta aaattgttac aaaaccacac attaaacctg ttgattgata tgcttttatt
     6661 gattcaacct ttatatattc aaccgtttta gaacttgaaa aagacgacca aatagttata
     6721 aatctaacac cccatgcaca aggaaaatac tgccaaatac cagaacccaa tccagttagt
     6781 aataatgctg aaagtaacat ttcaactata ccaactccaa ttgatgctcc ttttccgaac
     6841 cttaaactta agaataaatg taatatatat tcgaataaac tacacccaat taaaattagg
     6901 attgaaatca tataaaagtt ggatgtaaag taagcctcag atataccaaa tttaaaacct
     6961 agtactgaca ctaatgttgc tacaaaacca agacatacta agattacata tttacttata
     7021 agagtaagat attttggatt agatgttgtt aacatatgtt tataatttcc tgcataatat
     7081 tcttgttcaa cgaacattga acacacaaca cctgacaata aaggaaaagc tattgctata
     7141 acttgtaaat atgctactat ctttagtata ttatcagctc ctgaaacctt ataataccat
     7201 aaaaacaatc ccatccccat taaagcaatg caaatgtgca ttagtattat aaaatttcgc
     7261 tttaatttta ttaaatcaga acaaaatatt cttattagct gtctcatatt acttcacctc
     7321 tctactattg aaccaaaccc ttgtagctaa agcaaataat acaaataaaa caatagatat
     7381 taatactcct ggtaaaataa cagcattatt taaaagttca cttccctctg gtacaggaag
     7441 cccatttggc agaatatgca atacaacagc cattaatctt gaaggaatcg aatatggaaa
     7501 tatataccac atttctgtag ttgagaaaac agcaactcca acaatagtaa agaacatatt
     7561 tgttagaact gtaacaaaaa taccaaattt tgaagctaaa aacatacaaa gaggaatctg
     7621 ccacataaat agtaagataa ttaatatagt tgcaataata ttttgactta ttgaaatagt
     7681 ttctttatag attatcccaa caaaaactac acctattgca gaaataattg atgctataaa
     7741 acaacaaata atacagtaaa tcatttttcc agtccaaacg tctttcattt caattggcaa
     7801 tggtaaaata ccacgatatt tacttttttc atcttttaat actattaaag aacatattaa
     7861 tgttaatgct cctggaagaa atgtcgtata ccaccagttg aaagcagcat tttgccctcc
     7921 acctagtcca caaaaaagta tacaaaacac tggaactatc catatcaact tcattccaaa
     7981 agttcctttc atcttttgaa attccactct aataaatctt tccatgttac tcccctctct
     8041 ttctattttc agctacaact ttcataaaaa gttcttctaa gttttgttct tttgatactt
     8101 ctccttgata acccattact ccattagaaa ttattccgat ttcatctaca acttgtttaa
     8161 cttcagacaa gatatggctt gataaaataa ctgtaattcc ttgttttgga aaagaacgaa
     8221 tcaattctct aagttcttca ataccaatag ggtcaagacc atttgtaggc tcatctaaga
     8281 ttaaaagttt tgggtgattt aataatgcaa tagcaatacc aagacgttgc ttcatgccta
     8341 gagagaattg acctgaacgc tttttaaaag tatctcttaa atcgacaatc tctaaaactt
     8401 cttcaatgcg agagtcagga agaccaagta aagtagtatg gacttttaaa ttttctcgtg
     8461 ctgttaaatt ctcatatata gcaggagact caataagaga accaatattt gataaatctt
     8521 ttctactcca tctatgtccc tcaaacatta tttttcctga agttggattt aaaatcccag
     8581 ttatcatttt taataaagta gatttcccag ctccatttgg tcctaataat ccatatatag
     8641 aattaggttt aattgctagt gaaatgttat caactgccat ctgctcctta aattttttag
     8701 aaactccttt tacctgcaaa atataattac tcataatttt catcctttct tttgtcaaac
     8761 ttatttacaa gaatcattct aaatcataaa tataaggatt ttataagaat taaaaaacaa
     8821 aaaaagatgt gttaaaaaac acatcttttt tatcttaatt ctattaattt tccattgtag
     8881 tatatctcta cttcttcaat ccaaatgcta ttatcttctt cttggatgac atttttaata
     8941 atattattta tagtatatat tatattttgt tcatttaaat aatcatcttt ccaagaaact
     9001 ctactttttt tatctggctc tatcaagttt attttaacaa gattatcttt ttccttaact
     9061 tctacttcca tgtctaaacc attaaaatac tcatttgata atgtatctaa gattaatcgc
     9121 aatttatcct taagtgaact ttcttcatca atctgtaagt actttactat tttctcatcc
     9181 tttgagatta tctctatagg tattttagta gttttcttag aattaatcat tttactagtt
     9241 gtttcttctt ttgcatcttc atccttcttt acaactgctg attctttaga atcaactagc
     9301 tcaactgcac tgatgctata atccatacat gctgtactca ataaagtagt agcaattatt
     9361 aaaaaactta ctgctatttt attcataagt atccctctcc taattctctt tcgatactta
     9421 ttataccata gtatagatta tatttttata cttttaaatc aatttataaa ttattatagt
     9481 aaattattta caattctaag ctatttgaat ttatttttat agtatattct ttctctaaat
     9541 tcgacagatt tttattcaaa agccctttta tttccctcgt atttctatac ttgttgctat
     9601 gttcttcact atgttcataa tcatatgaat cattgtcata ttcttcatca ttattaaatt
     9661 cttcgattac ttcttttgac tttatatcac aattaaattt atacttattt actggtatat
     9721 caagtcttac cttagaattt ggaacttcta tgtctaagaa cttagcaatt ggcatattag
     9781 agtacaagta aaattcacta tcataatttg aatttctacc tattttctct tttatattta
     9841 cattttcagg tatatactct tctatattat cgataaatac atcttcattc tctgaagata
     9901 tatccaatga ctctgattct atgtttacat ttttaacacc taaaattgat tttgttgaca
     9961 agtgaagatt gctattagat attaagttta agttttctaa tctatttact ttgttctctg
    10021 ttatagctct gctaaaacct ccagaagaag ttttatatgt tatattattt gaagctacat
    10081 tatcgtatac aaataagtat gaacttatac ttgatgcatt tatatctaca ttatttggta
    10141 catatattat aatttctcta taatcatgtg aaaacatact atttatagat gtatttaata
    10201 attggtcgaa atttttaatc tttttaattt tattttcgta ttttctttca ccttttacaa
    10261 ctaaagtctt atcttttaaa gttacattgt atttgtaaaa ctcattattt cctcttgttg
    10321 tcacacgcac atcttcactt gaacttttct ttattgtaac gtttatattg tcaacagata
    10381 tatctaactt gtcaatattt actttttctt gataaagttt attttctttt ttaaattcag
    10441 cttccttttc taaaccaaag tttattaagc ttggcataac taaaatccct gaccatgttg
    10501 tccctattat acctattacc aataaaaccg cagcaaatat agcaagtttt ttattcatgt
    10561 ttatcttctc ctttcttatt gtctatatct tcaaggtcct tatatatcgc ctcaaaattt
    10621 atactatcat cattatattc atcagtttct tttatacttt cttcttctct attgtagtaa
    10681 tcattattat atgaatcttc attaatgtct ttattcaaat cttcatcatt taaagtagct
    10741 ttttttcttt tttccatctt ttcatttgcc tttatataaa tattttttct ctttatccaa
    10801 tgtatataat tatttagtaa ttgtttacct agttttatgg caaatagtaa aatttgccat
    10861 gcaagtatct caatccctac aaatgctatg gttgcgaaaa tacaaaacat cataacatct
    10921 ggaaccattg ctataaattt aatcacaaat ggaattaata taacaaaagc aattaatgaa
    10981 catactaatg caatagtaac agatacaata gcacatacaa acgcaaatgc tggaacaatt
    11041 agaaataaag ataacactac taataatccc tttataactt gtctagataa tccccctaca
    11101 ccttcttcta tacttggtgt aagtttatca tttatatttt cttttgattt attgtacttt
    11161 tccttagctt catctttcaa gatagagtaa tcctttttta tatttgattt aaacctacta
    11221 aatttaccat ttaatatatc tgtattcatt atttttccat ctatattttc ttttgattta
    11281 ctatactttt cttttgcttc atcttttaag atagagtaac cttttttcat atctgattta
    11341 aatttactaa acttatcatt caaaacatct gtatttatta ttttttcctc ttcaatattt
    11401 ttttctttag tctgcaagat aagttctttg gctatagatt ttggtgaacc aagtgatgct
    11461 attatctcca tatcactctt tccctctata agaccatcta cgaaatactc ttcataatct
    11521 ctcaatatat cattaacttc gctctcagaa aaatcttttt ttagataatc ctttaaaatt
    11581 tcaagaaact cagttttatt caagttcatt acctccctca tcttcactta ttaaaatctc
    11641 aacagccttt acaaattctc tccactcttc catcagattg gataaattct cttttcctag
    11701 tgaagttatt ctatagtatt ttctagcagg accttcatta gattctagta tatatgtttc
    11761 gaaatacaaa tcttttgtaa gtcttcttag aataggatat attgttcctt catttacatc
    11821 tattacttta gagatgttat gtactatctc atatccatac atatctttct tagatatgag
    11881 agcaagtaca caaatttcta aaacaccctt tctaaattgt gtgttcatat actccatcct
    11941 ttctttaaat acacagtatt gtgttttaca aggtactatg ttaaaacttc ttatatcctc
    12001 ataataacac agtactatgt tctgcacaat agttattttt aaatattctt gttgattaaa
    12061 tccttctata aatgtataat actctaatca gatatatttt atgaaagtgg tgataaattg
    12121 actaaaatga aagatgataa aacaatatta atcttaactg ctcaatttgg tgctggacat
    12181 ataagtgctg caaaagcagt caaagaatgt attattgaaa aatatagtaa ctataatgtt
    12241 gttatacaaa actttataaa tgcaagtatt cccatgatga ataaacctat ggtaaaactt
    12301 tatgaaaaca atacaaaata tactccggga ttatataatt actattatta ctttaagaaa
    12361 tcgtttgatt caagacatga tttatctcat aaattatata cacctaaact ttctgagtat
    12421 attgctgata taaatccaga tttgattatc tctacatttc cactagcggc tgcttgtgta
    12481 aataacttta agataaaaaa tcctgatata aatataccta cacttacagt tataacagat
    12541 gttgtagata gtatggaatg ggtttttgaa aatacagatt tgtattttgt tccttctcct
    12601 gaaataaaaa atagattttt tcagaaagga ataaatccag actccataaa ggttactgga
    12661 gttcctgttg ataagagatt tcaaattgaa agtaaagaac tatgttgtga taaatataga
    12721 ctattacttc taggtggagg aagaggtcta tttgatatag atgaggattt tatgcattgg
    12781 atagatgaat ttattgaaga acatagtaat tctatagaaa ttacaatagt tactggtaaa
    12841 aacaaaaaat tatatgacaa tctaactcat aaaaaacctc taaaaaatat taaagtactt
    12901 ggatttgtaa acgacatgta taatctaata agagagtgtg acctaatgct aactaagcct
    12961 ggaggagcaa ctatatttga agctattcaa tcacaaactc cagtactcgt aaaaatgcca
    13021 aaagttggac aagaaattga aaatgctaaa tttataatag ataagggtct tggcatgatt
    13081 tatagtgatg acctagacct taaaaacata ttttataaat tagtatcaaa tgaatttgat
    13141 tctataataa actttatgaa aaaaaattta gaggaattta aaacagtcat tcatccagag
    13201 aaaattgctg attatatatc agaattaata gataatcatt acagttaaat tgtactatta
    13261 aaaaatttca aacataaaat taaaggcaat caagtatgct tttacacttg agaatttact
    13321 catagtgttt aataatactc gattgccttt atcatatcaa aatcaaattg ttatattagt
    13381 atatattttt cttgtactgc atatacagtt gttgtatttc ttccatactt ctctttaatt
    13441 ctatttgtaa atctgaagca gtctggtaat ctttattttc tatagcttct ctcactctag
    13501 tgaatagtga ttctctagtt aaagtatcct tcataatatt gaattttaaa tccgatattg
    13561 agttccaaac tacttcatca agcgcatcca tattttcttt tgtgtgtatc ttgacaatac
    13621 ttcttatttt ttgaatccca gcctctatta ttctagtttt taatttcttt tgccacttat
    13681 ctatagctcc aatcttaaat gattctatta tactatcagt aaatacatca ccttgtttta
    13741 aagattttaa ttttgaagca tatatttcta agtttttcat attttcataa actgtagctg
    13801 gtggtattcc aaatcttgca tttctttctt ccaaactata atgttcaaat acatcatttt
    13861 catctctata tgctctatct ttttctaaat agaaactttc ttcacctaca tttttagata
    13921 actctttttc aagttctttt gtactaagac cactcttagc tgctgctttt ataccatcca
    13981 acattgtttg ataacatcct gctattacta aatacgtgtt agatagaggg cttggtgacc
    14041 ttaactcaaa tcttacagtc tttggatttt tcatatctct tattaatcca acaagcactg
    14101 acctatttct tgatggaatc tcataactat gtcctaaaga tgtaactatg catacaggag
    14161 cttcaaagcc aggaactaat ctattaaatc catcattaga agctgttaca attgggttta
    14221 atacttcata attgtaaaga agtcccataa gagccccata tcccagttca cttagataat
    14281 cttcttttaa atcttttgga gcaaataaat tttttatact tccatccttt aacttagcac
    14341 ttactcctac atgtgtatgt ccaccacttc ctgctactcc atgaattggc tttgctttaa
    14401 atgtaacttc tagtccatgt gatgtaaata catcctctat aacatctctt actaataatt
    14461 cattgtctgc tgcttgtaat ggtgtagaaa acttccaaga aacttctaat tgctccatag
    14521 catggtttgt tcttccatct atactaatag aactttgtat tcctccgacc tctttatgag
    14581 ccatttcagg gttcacacct agtttttgaa gtataattaa acttttctcc aaacaagttc
    14641 ttataatacc atgagttctt ttccagtatt gctcttttag gctttgtgat acatataatt
    14701 tctctaggtc agctttatcc tcaggagtat taacccagaa ctcaagctct gtagctgctg
    14761 ttaacattat ttcctctata tcatctactg aatctattcc tatgttattt attacatgtg
    14821 gatattctct aaatatttca tacattgact ttttaaagta cttatctgct ttttgaagta
    14881 ctcctctaga gcatactttt ttattatcat gtattaaaaa tgcaggtatc tttaatgttc
    14941 caacaggtag acctacttct tcgtctatat attccatatt gtaatctaca taccaatgac
    15001 aacttttatc tggcattaaa tctacttttg cattatttaa tgtagctatg ttataaagct
    15061 caacacttga accatcagtc tgtatagctg attccaaaaa tttatctata tcctctaaaa
    15121 ataattctac aggaatcttt tcatctgttg catttcctcc taaatcaacc cccattaagg
    15181 aaacaaatct tatattttta ttgcttttta ggatttctct taattcttca ttagtgtgtt
    15241 tatctttctc tattacgtat aatagactat ccatttttcc actctcccat aatttttatg
    15301 tatattttat tttgttaaat acaagataaa tttcattttc cttacattta tgaatttttc
    15361 ttgcaaatca ttcgtgtttt attcgtgttt ttatttatat tattatatat caagtacaaa
    15421 aagttcgcaa tattttttat tggaaaaaat attatacata caatagttta acatttttac
    15481 aaatatgggg tatactaaaa taaaagtatt tttatttgaa aatttaattt tatttataat
    15541 ataatctata gtaaaagaaa taataaaaaa atttaggaga taaactttat ggaatacata
    15601 attttcgatt tggaatttaa ccaaggattt gataaaaaac tcaataaaac tgtatccaat
    15661 gaaaagtgtc cttttgaaat tatacaaata ggagcaatta aattggattc taaattcaat
    15721 ataatagata cgtttaatag ctatgtaaaa cctacaattt ataaagacat acatcccttt
    15781 attagtcgta tgactaatat taaaaattct gattttaatg attcgcctac tttccctgag
    15841 gtctacaaca attttataaa atttatatca tcacaagacc ctatattatg tgtttgggga
    15901 gctggagatt taaaggaatt atacaggaat atcaactacc acaagctacc atcaaattcc
    15961 ttacctaaat cttatattaa tatacaacaa catgcatcta aatatttcaa taatccagca
    16021 ggaaaaagca ttggcttaca aaatgctatt tctatattgg aacttgatga aaaaatgtct
    16081 tatcacaatg ctttaaatga tgcatattat actgctaaag tttttattaa gatatacaat
    16141 ccaagtataa tcccagatat atatctttat acgagcatca aacctaaaac tataagatat
    16201 tctaataaaa aaagagttga ttatgataag ttatttgatg aatttaggaa aattttaaat
    16261 cgtgaattaa ctaaagacga gaaaaaaatc attaacttag cttataatat ggggaaaaca
    16321 aatcaattta cattagaaaa tgttaagcaa agaaaaaaca aatagagttt atttagataa
    16381 aaaataggca tgtagattaa aaaactagtt aactttaaat ttatatctac agcccaattt
    16441 ttatcgtaat taatttttat tttatctcta attcaactgg acaatggtct gaacctaata
    16501 cttcagtgtg tatatccgca cttaccaatc tatcttctaa ttttttggat gcacaaaaat
    16561 aatctattct ccaccctgca ttattttttc ttgcattaaa tctatatgac caccaactat
    16621 agacaccttc tttatcagga ttaaaatatc tatatgtgtc tataaatcca ctgtctaata
    16681 atttagaaaa cttctctctt tcttctttag taaaacctgc atttttcata ttatttttag
    16741 gattttttaa atctatttct ttatgtgcaa cgttcatatc tccacacaca attacaggct
    16801 tatgattatc tagtttggtt aaataatcta taaagtcgtc ttcccatcta gttctatatt
    16861 ctagtctctt taactcagat tgagaatttg gcgtatatac tgtaacaaag taaaaatctt
    16921 caaactctag agttatcact ctaccctctt tgtcatgttc ttctatattt attccataca
    16981 ttacttttaa aggctctttt tttgtaaata tagcagtacc tgaataaccc tttctctcag
    17041 cataattcca atattgaaaa tatcctggta aatctaactc tatttgccct tcttgcaatt
    17101 tagtttcttg taaacaaaat acatctgcat ctacttcttt gaaaaagtct aaaaatcctt
    17161 tcccaacaca agctcttatt ccattaacat tccatgatat aaatttcata cttatactcc
    17221 tcatctaaac attgatacac tccccatagc taaagctggg ggcttttcaa caagtttata
    17281 aattcttaat ttacttttat tttaacaata ctataatttc atctaaaatt actatttact
    17341 taatacctaa aaatatatgt tttaatcaat ttttaacttt tttgttaact cttcagggct
    17401 cattgaagaa acatacatat ttgttccaag ttcaaatcct ccaaaattat attttcttgg
    17461 gattatgtgc atatgaacat taaaatacga ttttgtttca atctttgttg gatgagtgtg
    17521 aatacataag ttaaatggca tatatccatt tatattgtat aacttcttaa acaacttttc
    17581 aaatattgta gaaagttcct gtatattatt atcatttaat tcaccaaact ctattttatc
    17641 tttaaatatt attcttactt ctccactata cttggttgca tacggtataa gtaccaaaaa
    17701 agcttctcca ttataaacaa ctcttctatt taaacttatt tcattttcta ttaaatcatc
    17761 atacaaagaa cttttattgc tgtcataata ctcctttgaa accaacagtt cattcgtaat
    17821 ttcaggtgga ataaaagaca tagacaatat ttgagcatgt ggatgcatca aagatgctcc
    17881 tgcctttctc aagaagtttt tgaagatact aacatattct actttatcgt ctttcgacaa
    17941 atctctaaat ctatttctat acataaaaaa aacatcttca aattcttttt gactcatgtt
    18001 ataaaaacta cgattatgtt tattgctctc tatcattact tcatgaacac catatacatc
    18061 tcttgccata tcatcaatta taggaaattt attgtagaca gattttacaa gccatccttc
    18121 ttcactttct atttcaaata atgcatcagt tgcataagct tcatttcctt tgcaaaatgg
    18181 gcattcttca tcatattcat tagtttcttc atcttcattt gggatatctg ctgtatctaa
    18241 aggtcttttt aacctatctg tagcaaatat aacaacatca ttggtaattg gatctattct
    18301 taattctttc ataattaaca cctcttagtt aatagccttt aatataatta tatgatattt
    18361 atcgtatctg ttaaataaat ttataatatt ttttataatt ttgcatattt attattatat
    18421 attttgtcta tagctgaaag attataacta aaaaatcact atcagtatat cagaatatta
    18481 tcttactata aattaattta tgaatacata atttagtagc ataaacatgg gtattttact
    18541 ataatttgtt aaaagtttat tttaaagcta tattaatttt ttttgatgat ttttacccat
    18601 aagtatgtta ttattatctt gtaaagatgt cttgtattca aacatacaat ttacataata
    18661 caatatacgt atttaaatta aatagggggt attaaaatga attattcaaa cagagttagt
    18721 gcaatgcaag cctctccaat cagaaaactt gttccatttg cacaagcagc aaaagataaa
    18781 ggaatcaaag tatatcactt aaacatagga caaccagaca taaaaactcc taaaggattt
    18841 tttgatgctg ttaaaaactt tgatagcgaa gtcttagaat atgctacatc agaaggtatt
    18901 ccagaattat tagaagcact tcaaaattac tataaaactt acgatatgaa ttttgaaaaa
    18961 gatgaattat tagtaactaa tggtggtagt gaagctttac ttttcactat gatggcagtt
    19021 tgtgacccag gagataatct attagttcca gagccattct atacaaacta caatggattt
    19081 ggtcaatctg taaacgtaga agttaatgct gttacaacaa aggcagaaaa cggtttccac
    19141 cttccatcta aagaagaaat attgtctaaa gtagatgata aaactaaggc tataattctt
    19201 tctaacccag gaaatccaac tggtgcaatt tatacaaaag aagaacttaa tatcctagct
    19261 gaaatagcta aagaaaaaga tttatggata atagcagacg aagtttatag agaatttgtt
    19321 tatgatgggc ttgagtatac aagctgtggt aacttagagg gtgtacaaga tagagttata
    19381 ataatagaca gtgtttctaa gagatacagt gcttgtggtg ctagaattgg ttctatcgct
    19441 tgtaaaaata aaggtcttat cgctcaaata cttaaattat gtcaaggaag actttgtgtt
    19501 cctacattag agcaaatagg agctgttgag ttatacaaaa ctcctgtttc ttacttcaaa
    19561 gaagttaatg aagagtacaa aaaaagaaga gatgtattat acaatgaatt aatgaaggtt
    19621 gaaggtgtta tatgtaaaaa accaactggt gctttctata tagttgctaa attaccagtt
    19681 gaaaatgcag aagatttcac aatttggatg ttaaaagaat ttaacaaaga taatgaaaca
    19741 gttatggttt gccctgctga aggattctat gctactccag gtttaggacg tgatgaaata
    19801 agattagctt acatcctaaa tgaaaaagac cttcatagag ctgctacatt attaaaagaa
    19861 ggtttagagc aatatgtagc tttaaaaaaa taatttttta aacttaattt atataaattt
    19921 aatgatataa aatgtcccct tttcagatat tatttgaaaa ggggacattt cttaattaat
    19981 atattaatta agacttatac ctgactcttt ttcattttct ttatatagtg ttggccagta
    20041 atctttattc actttaatta aagcatctaa aacttgtctt gccttagttg gagaaattat
    20101 agttctattt aatgttaaag ataaaatagc tttttcataa gattgttcaa aatacgcatc
    20161 aactgctaac ttttcataag ctaattgacc ttctaacagt cccttataaa atgttggtat
    20221 attacctaca tgtaaaggtc tcggtccatt ctttgtaaga atcgcagcta cttctaccat
    20281 tgcatcatca ggtaagttat tgatagcccc attattttct gttataacta tagctatttg
    20341 ctgtttgtta tgagctattg cttctgcaac ttcaactata aattccccat gagcatcatt
    20401 atgcactaaa ttagaaccta tagatgttcc tttttctata acagatttac attcttcaaa
    20461 tacacgcttt tctcttccat tcataacttc tcttgctcta gtacaattta tatttgattt
    20521 agctactttt ttatcaggat atagataata ctgtacatat gtattaggta agtaatctgg
    20581 tacatcttcc aacatatctt taaccattcc ataagtatct agccaagatt tatctctttg
    20641 ttctgcatca acaggttgga atccagagtt tgctactaaa gcttttatct taggtaccaa
    20701 atcttcacca tttttatcat acaaatgtgt aaaccatccg aagtgattta aaccaaagta
    20761 tacaggctca aatgttcttg aatccatacc taacaatctt ccataagacc ttaacaagtt
    20821 aactggttgg tcacacatat taagcaatct tttatcatct ggaaatactc tttttaatgc
    20881 ttctgcaaca atagcagcag gatttgtata atttaatatc catgctttag gagagtattt
    20941 tcttacattc ttaaccattt ctatcatatc acctatagac cttaacccat aagcaaatcc
    21001 accagctcca caagtttctt gaccaaccaa tccaaattgt agaggaatct tttcatctaa
    21061 ttctcgcatc ttaagaccac cagttctcat ttgaacaaat acaaaatcta catctataaa
    21121 tgcttcatcc gggtttgtgg tatattcaag tgttgcttta ggatattctt ctttaaataa
    21181 tattttccca aattccccta ttaatctttg acgttcttcc actacatcta acatagttac
    21241 tttatttaga ggaaatctat cactcatttt acacaaagac tttaaaagtc ctggagtcca
    21301 agtactgcct cctccaacta tagctatatt gtatcttttt ttcattatta tattaatcac
    21361 ccctgtcttt tattaactaa cttgtaagat gattataata tataaagttt tctacatgaa
    21421 tacttttagg taatcattaa aaatagttca attttagtat ttttttcaaa attgtaattt
    21481 ttttcatcta ttgtattttt caatgtattt atgaaacata tattccataa gagaaagtgc
    21541 tggcactcta gatgtatagt taacactatt aaaattatat tgtggagaag atacaaataa
    21601 ttttatatca gctatatttg ataatgaatt gacacttata tttgtcagag aaattacttt
    21661 aacttgattt gtttttgcta tattacatgc cttaataacc tgagcagtct ctccagacat
    21721 agatacagca attacaaggt catctttagt aatatttgat gcagccaaat acatgagagt
    21781 tgagtcatca tagtatttta catttacacc tatagctgaa aaacgttgag acatttcagt
    21841 acatactaat ctagaggacc caacaccaaa aaaatatata tgcttagaat tatgtatttc
    21901 atttaaacac atatctatat tctctacatt tattaaattc tgtgtgtcca taacatcagt
    21961 aaaaatatca ctttgacttt tagtaaactc cttatttctt tcatttctat ttgaatatga
    22021 aaaaactaaa aagtttttaa attcactata tccagaaaag ccgagtttct gacaaaacct
    22081 tactatgtat gcagtcgaat taaaagtttc atttgctata tctttaatct ttttttgatt
    22141 tattttttca gaatttttta gaaaatagtt taaaagagat atttcatttg tagataattt
    22201 ttttcgattt atatctaatt tgtctattaa gttcatttat acctccataa taatgatttg
    22261 tagttaaaca actttctcat atttattata taaatataca ctatacagca tttatcttat
    22321 gtttattata ctacaaaaaa gacaatacaa attataaatt agtattgcct tttaatattt
    22381 actttaaata gacattgcaa aagaaacttc gtctcttact tgagatacag atggcccata
    22441 aattatttgg atatctttat cagatacttt tataactccc attgcacctg tatatttttt
    22501 taaagcattt tcatctactt tactcatatc tttaactact actcttaagc gagttataca
    22561 gtttgttata tcttctatat tgtccttacc acctaaatat ttaattatgg ataatgctct
    22621 cttagtagtt tctccacttt gagctatttc tactttattt gataaaggtt ctaaatcaaa
    22681 atttatatcc gctatttctt ctcgtcctgg agtttgtaag ttaaatctaa gtatcaaaat
    22741 cttaaataca ttataataaa ctagtgcata cactaaacca agcattatag aataaatcca
    22801 ctttgtattt ggttgcataa ctccaaatac tactaaatct attatgccac cttgagtatt
    22861 tcctattgct actcccaata tatgcatcat cataaatgat atacctgata aaaatgcatg
    22921 tataacaaat aatattggtg atacaaataa gaatgaaaat tccaatggtt ccgtaattcc
    22981 tgttacaaat gttgctaaag caccagctag taatatacct ttaaccttcg acctattttc
    23041 aggctttgca gttttataca tggcatatgc tgctgcagga agaccaaata ccattatagg
    23101 tattttccct tgagctaaaa atctagttgc ttctgttgaa aaactgattg ttttagggtc
    23161 acttagttgc gctaagaata tgttcaatgc tccaaatact tcttgccctc ctacaactaa
    23221 agaacctcct acttcagtaa atctaaacat agcatttaat atatgatgta gtccaaatgg
    23281 tattaaaagt ctttcaagaa atccaaatgc aaatgttcct aaatatccta aactagctat
    23341 accaatacct acactctgaa taattgtatt tacatatggc catatatatg gtattattat
    23401 ccccaaaata gagaatacta ctgttgttat tattggaaca aacctagttc caccaaaaaa
    23461 tcctaaataa tctggcagtt ttatattgta gtatttatta tgtaatgatg ctgttattat
    23521 accaactaca attccaccaa atactcctaa ttctaatgtt tgtatgccta aagttacagc
    23581 ttgacccatt tctctcatca tgtcaggttc agcaagaata cctctacacc ctaacatata
    23641 atttacacta gtatgtaata ctaaataacc aacaaaacca gaaaatgcag aaactgcttt
    23701 ttcttcttta gcaagaccaa cagatattga tatagcaaag aatattgcta agtttccaaa
    23761 aagtactcct gttatagctt ttgtaaaacc caatattcct tgaatgaaat tatttgctaa
    23821 aaaaggtata tcactaacta tattaggatt tgaaaatgct gaacttattc ctaaaaatat
    23881 acctgctgca gatattaatg ctattggcaa taaaaatgat tttcctaact gcaaaaagaa
    23941 ccctttcatt ttttgtttca tttctatccc tccattttta aatttttaaa acgttttcta
    24001 tgtcataatt atatattttt attttgtttt tgaaacttta taatttgttt taaaatattt
    24061 ttacataaat agtaactttt tcaaaactgt aaattttttc aaacaaagac atagtaacgt
    24121 tgtaacatat ataaatttaa aaagagatta gaaaataaaa tcattgaata taaaatagaa
    24181 aaagatagat ttcgcaaata gatatgagta ggtactgaca aatactgaaa tttataagaa
    24241 attataaaaa agtacatcga aaaaatttaa gatgtacttt ttagattgta tttagattta
    24301 taattatata gctaaatgta taacaaagaa ttaatcttaa aactattaaa atttttgtat
    24361 ttttctatct aaattcttct ttgttcaata tatattgaag agtatttatt gcctctaaat
    24421 attttcttct atcttcctca gtcaatttag aaagcttttc tcctaaacca tcactcaact
    24481 tatccataaa atcagcagct atcttatctc cctcctgagt aatatcaatt agatattttc
    24541 ttctatcaac taaatctctt tttcttgtta catacttctt atcagttaaa tcatcaacca
    24601 tactagttaa actgcctttt tctatattta atttagagca taaatcagtc atacttatta
    24661 ctccataatt ttttatgaaa actaatgctc ttaattgtgt tttatttaca ttataatctg
    24721 gtgcaaattt ttttaggtct tctaagtaca ggctagaata aatcttagga aatgttttta
    24781 ctaaaaaact tatggtatct tttactatat agttcatata atcacctcgg ttgttttaaa
    24841 atttattaaa ttatctaaat aactttattt cagtttacat taaaagtatt gcaagtacaa
    24901 tagtttttgc tgaatgttta aatttaatta gtaatcttat cattttttta tttttattta
    24961 tattattctt attaaatact atttatatag ctattttatt aaatttatgg tatagtaagt
    25021 aaagaagaac tcttcacagc ggtgaagaga agacgctagc ccattaaaat ggctatctta
    25081 tttataaata agatagccgt ttttaattat attttatctg tacaattcta tactcttagg
    25141 tctacatcta cttttcctaa gtcttcctta taatctttta tagcaggttc tacaacttca
    25201 tttacaaatt ctacaacttg ttctggagct ctacctataa agttttttgg gtccattata
    25261 gatagtattt cttccttact cattttaaat gaatcatcat tcattataag ttcaattaag
    25321 ttattatcct taccttcatg ctttactcta taggcagctt tcatagaata ttctcttatt
    25381 atttcatgta actcctgtct atctccacct ctttttacag cttccataag tattgtttct
    25441 gtagccataa aaggaagttc ttcatttaca cgtttattta tcatattttc atatactacc
    25501 aagccatcag ttacatttat ccctatttca agtatagcat ctgctgccat aaatgcttga
    25561 ggtattgcca atctcttatt ggctgagtca tctaatgttc tctctaacca ttgagtagct
    25621 tgtaccattg cagggtttat agattcagag attatatact tagataaaga tgctattctc
    25681 tcacttctca ttggattcct cttataagcc attgctgatg aacctatttg atttttttca
    25741 aatggctctt ctaattcttt taaactttgt aaaagtctta tatcattagt catcttatgc
    25801 atactttgag ctatgcctga tagtatgctt attacttgat aatctaactt tctagtatat
    25861 gtctgtccac ttactgcata ggaagaatgg tatcccatct ttttacaaat tttcttatct
    25921 aactctttta ctttttcgtg gtctccttca aataagctta aaaaacttgc ttgagtacca
    25981 gtagtacctt taactcctct taacttcata ttgtctattc tatagtttaa atcttccaaa
    26041 tcaagaatta aatcttgagc ccataaacaa gctctttttc ctactgttgt aagttgagca
    26101 gcttggaagt gtgtaaatcc aagagctggc atatctttgt acttaagtgc aaattctttt
    26161 aaattgttta ttaaatttat aagctttact cttagaagtt ttaaagcttc gttcatttgt
    26221 ataacatctg tattatctcc aacatatgca gatgttgctc ctaaatgtat tattcctttg
    26281 gctttttcac attgtaaacc ataagctttt acgtgagaca ttacatcatg tcttacttct
    26341 ttttctattt ttctagcttc atcaaaattt atattatcta tattagctct tagctcatct
    26401 aattgttctt cagttatatt gattcctaac tcatgttcac cttctgcaag tgcaacccat
    26461 aaccttctcc atgtagagaa tttaaattgt ggagaaaata tataactcat gtccttgcta
    26521 caatatctgt ctgtaagtgg gtttgagtaa gtagtatatt tattatctat cattatatta
    26581 gcaccttcct taaatctttt gtcgtgtgat tactgtgtac acattagtac ataaataatt
    26641 atatataatt ttacgaacta taacaagact tttttatttt ttgttcacat ttatatatag
    26701 tgttttttat ttattactat tttttattta tatctaaatt atctttttca attaagtaat
    26761 ccactatttc actatcaaca aaatttaata catacttata tctaattgca tagtatttat
    26821 cactccatcc aagctgattt tttacatcac tttcatctgc cccaagcctt aaacttattg
    26881 ctgcaaatga atgtctaaag tctcttgctg aatattgaga aagcccagct aaatctaatg
    26941 cttttttgac tataagtctg acatttctat ctgttataga attactcttt tgagttgtaa
    27001 aaataaaatc atcactaggt attattacct ctggaagccc agaataattc ctgtaatctt
    27061 ctaaaagttt aaaaaagtat ggatgtaact taactactct ttcttttctt cccttaccta
    27121 accttacata tgagttatcg ttttcatcta ccataaggtc tttccacttt aagctaacta
    27181 attcatttaa taaacaccca gtagtaacta gacagactat tattactcta tctcttatat
    27241 ttaattttgg taggatacct ataagtttat ttatttcttg tatacttaga acatcatctt
    27301 ttgtctttat tcttgttact gtgggttttt ctacatatct aaatggattt atatctatgt
    27361 atccttcttt ttttaagaag ttataaaaac tatgtaaata actatatatc ttttcacaag
    27421 ttgatttcgc atatttagtc tgtatatagt ctacaaaatt tttacaatct tctttcgtag
    27481 cataaatcaa ttctttccct tttaaaaatt ctttaaacag tataaccttt gataaataat
    27541 cgtgtttcgt gtttggattt aattttgtag aaaaaacatt aaaagtttca tactcataat
    27601 catctagtat ttttgtacta tacataataa ctcctttcct tttcataaaa taaaatagct
    27661 atacttttac tataccatat atttccccct caaatatacc ccttattcaa tagataactt
    27721 ttaagtttat tttgttatat tatgaaggtt ttatgaaaat atcaatcttc cattgtgcac
    27781 tacacagtac tatggtatta taagttagta aaaggtggca tttttttatt aatctatgaa
    27841 taaaatatgc tactataaat ttgtattgga ggtattttga tttggaaaca tttagtttgc
    27901 ttgaaatttt taaggcagtc attttaggca tagttcaggg aataacagaa tggctaccag
    27961 taagtagtac agggcatatg atattagttg atgagtttat aaaattgaat ttttcaaata
    28021 catttataag tacattttta gtcgtaattc aatttggttc aatattagct gtacttgtta
    28081 tattttttag aaagttaaat ccttttgaca gtgcaaaaaa tattaaacag aaaaaagaaa
    28141 cagttcgttt atggcttaaa gttataattg cagttatacc atctggagtt attggaatac
    28201 tgtttgaaga tgatatagat agattattct ttaattcaac agtagttgct attgctctta
    28261 ttgtctatgg tataataatg atagggcttg aaaagagaaa taaaagacct aagtataaag
    28321 atttttctca agttacatat aaattagctc tttgtatagg attattccag tgtctagctc
    28381 ttataccagg tacatctagg tctggttcta ctataatagg tgcagtatta cttggtactt
    28441 ctagatatgt agcagcagag ttttcattct tcttagccat ccctacaatg cttggagcta
    28501 gtgcattaaa acttctaaaa gctggatttg gatttactgg atttgaatgg cttattttag
    28561 gtgttggttc tgtagtagca tttgttgttt caattgtagt tattaaattt tttatggact
    28621 atatcaaaaa acatgacttc aaagtatttg gttattatag aatagtcttg ggtatagtgg
    28681 ttcttgctta tttctttcta ttataatgta ttctaattta ataacaaatg ataaggagaa
    28741 aagttatgag tgttaaaaaa gaaatatttg attggattaa gtcaatagct atggctattg
    28801 tacttgcatt tgtaattcta caatttataa gaccttctat tgtaagtgga gaatcaatgt
    28861 atcctacttt agatgataaa gattatctga ttttaaatag gatatcatac aaggttggta
    28921 aacctgaaaa aggcgatatt gtagttttta aaaccaattt agttgatgga gaaacaggaa
    28981 agaaaaaaga cttaataaaa agagttatag ctactgaagg tgacagaata aaaatatcaa
    29041 attctaaagt gtatgtaaat ggaaaattat taaatgaacc atatatacac aataactata
    29101 cttctggaga tatagatact gttgttccaa aaggtaagct atttgcaatg ggagataata
    29161 gagaaaatag taatgatagt agattccctg atgtaggtat ggttgatgag gatgaaattc
    29221 ttggtaaggt tatggtgaga ctattacctc ttgataatat tgggaaagta gactaaaaaa
    29281 taagagactg aattggtaaa gtttatttta caaatctcag tctcttttta ttttcttaaa
    29341 ttaaaaatat ttttttacta aatatctttt gattctgtga taagtcttat tatatcactt
    29401 gcttcttcat taaaagtcgt tactatactc aggtagtctg ttgctctact catagctaaa
    29461 taaaccttat ttaaatctcc tacaaagtca tttacttgat agtcttgctc ggtatcattt
    29521 atagtttgat tatataacat ctctaattca catactatta ccgctttata ctctaaattc
    29581 tttattgcat atatatttgc tattgtagtt cctaccttct tacttatatt tgtcaaatta
    29641 tcttctgcac atatataagg aatttttgcc tcttccaatg ctttttttaa catatattga
    29701 aagtaaatgg tttttccact ttttaatttc ttcttattgt aaggataaac gatagcaact
    29761 tcagaataat caagaccttt tttacttatt aaatactcaa tttcccagat tacagaactt
    29821 atttggtcat caaggtcact tacttttatt atatcaacag acttatttcc acctctaagt
    29881 gctttagtat aaaagaaagt actaaattct gtatttggtc ttaattcgtt gatatatgag
    29941 ttagaattat ttgaaaattt atttgtaaaa tctacaatct ctttagcttg tctataattt
    30001 ttacttaaga taatcttatc atcaaattct ataccattat a
//
LOCUS       DAEMAE010000018        29882 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEMAE010000018 DAEMAE010000000
VERSION     DAEMAE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.018"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(767..849)
                     /locus_tag="KPH93_000711"
     tRNA            complement(767..849)
                     /locus_tag="KPH93_000711"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(813..815),aa:Leu,seq:caa)
     gene            1168..3816
                     /gene="polA"
                     /locus_tag="KPH93_000712"
     CDS             1168..3816
                     /gene="polA"
                     /locus_tag="KPH93_000712"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG1687548.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDIEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            3825..4427
                     /locus_tag="KPH93_000713"
     CDS             3825..4427
                     /locus_tag="KPH93_000713"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG1687549.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            4420..4974
                     /locus_tag="KPH93_000714"
     CDS             4420..4974
                     /locus_tag="KPH93_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG1687550.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKVLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            4974..6689
                     /locus_tag="KPH93_000715"
     CDS             4974..6689
                     /locus_tag="KPH93_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1687551.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITRYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEINLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNVTFISLAVRNNYIVSNSRLKGEITPNDFDVYEGISNLDIK
                     SNESN"
     gene            6762..6974
                     /locus_tag="KPH93_000716"
     CDS             6762..6974
                     /locus_tag="KPH93_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG1687552.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            7175..7774
                     /locus_tag="KPH93_000717"
     CDS             7175..7774
                     /locus_tag="KPH93_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687553.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            8002..8373
                     /locus_tag="KPH93_000718"
     CDS             8002..8373
                     /locus_tag="KPH93_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1687554.1"
                     /translation="MKFNFCHNNLNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            8505..9779
                     /locus_tag="KPH93_000719"
     CDS             8505..9779
                     /locus_tag="KPH93_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1687555.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKNNGWYKVKLSSGTIGWVSASYISETSEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            9890..10834
                     /locus_tag="KPH93_000720"
     CDS             9890..10834
                     /locus_tag="KPH93_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687556.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLMEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            11050..12387
                     /gene="rsxC"
                     /locus_tag="KPH93_000721"
     CDS             11050..12387
                     /gene="rsxC"
                     /locus_tag="KPH93_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG1687557.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            12410..13387
                     /locus_tag="KPH93_000722"
     CDS             12410..13387
                     /locus_tag="KPH93_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG1687558.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            13387..13956
                     /locus_tag="KPH93_000723"
     CDS             13387..13956
                     /locus_tag="KPH93_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG1687559.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            13969..14562
                     /locus_tag="KPH93_000724"
     CDS             13969..14562
                     /locus_tag="KPH93_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG1687560.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            14577..15152
                     /gene="rsxA"
                     /locus_tag="KPH93_000725"
     CDS             14577..15152
                     /gene="rsxA"
                     /locus_tag="KPH93_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG1687561.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            15168..16139
                     /locus_tag="KPH93_000726"
     CDS             15168..16139
                     /locus_tag="KPH93_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG1687562.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            16359..16943
                     /gene="maf"
                     /locus_tag="KPH93_000727"
     CDS             16359..16943
                     /gene="maf"
                     /locus_tag="KPH93_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG1687563.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            16988..17647
                     /gene="radC"
                     /locus_tag="KPH93_000728"
     CDS             16988..17647
                     /gene="radC"
                     /locus_tag="KPH93_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG1687564.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            17768..18778
                     /locus_tag="KPH93_000729"
     CDS             17768..18778
                     /locus_tag="KPH93_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG1687565.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            18788..19681
                     /gene="mreC"
                     /locus_tag="KPH93_000730"
     CDS             18788..19681
                     /gene="mreC"
                     /locus_tag="KPH93_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG1687566.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PINLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            19696..20184
                     /gene="mreD"
                     /locus_tag="KPH93_000731"
     CDS             19696..20184
                     /gene="mreD"
                     /locus_tag="KPH93_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG1687567.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSHNISTLFVKGLLVAPIVNSLIGLVIYRLSKKAVLKLK
                     ED"
     gene            20196..23174
                     /locus_tag="KPH93_000732"
     CDS             20196..23174
                     /locus_tag="KPH93_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG1687568.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEERAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPG
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEDV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRHVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            23272..23955
                     /locus_tag="KPH93_000733"
     CDS             23272..23955
                     /locus_tag="KPH93_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG1687569.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            23979..24776
                     /gene="minD"
                     /locus_tag="KPH93_000734"
     CDS             23979..24776
                     /gene="minD"
                     /locus_tag="KPH93_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG1687570.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            24794..25078
                     /gene="minE"
                     /locus_tag="KPH93_000735"
     CDS             24794..25078
                     /gene="minE"
                     /locus_tag="KPH93_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG1687571.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            25163..26293
                     /gene="rodA"
                     /locus_tag="KPH93_000736"
     CDS             25163..26293
                     /gene="rodA"
                     /locus_tag="KPH93_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG1687572.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLVLLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKIR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            26334..26747
                     /locus_tag="KPH93_000737"
     CDS             26334..26747
                     /locus_tag="KPH93_000737"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG1687573.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRV"
     gene            27397..28431
                     /locus_tag="KPH93_000738"
     CDS             27397..28431
                     /locus_tag="KPH93_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG1687574.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIMVAYNHIKNNTTYVDWNA
                     AEGANTAYTLVTKKGACSGMCRSMKALCDAMGIECYYVHSIANDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLAHPLPFDKNAYPAVGAVKVQKPASPGKHTMLADQEGAIL
                     NEVYGNNMKPQNKMYEGVPCSSIDSSINTIKFTSSNSNLDPSKHTISVIGFMKTPYGM
                     VALAPDSNPVSYPFEYDKPFSLDFTGKNRSAAELSKYCKKNNIIIKVEIGETASNSGR
                     GMVAYARYVN"
     gene            complement(28528..29721)
                     /locus_tag="KPH93_000739"
     CDS             complement(28528..29721)
                     /locus_tag="KPH93_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG1687575.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT        11695 a         3456 c         5611 g         9120 t
ORIGIN      
        1 atgaatattt ttatggatat tttatcacaa aaaaataacc cttttacaaa taagggttac
       61 tttttttctt tattttttct tattatatca ttattttttg tttatttcaa tattttgaca
      121 ttttttccat tatagtgaaa atatatcttg agcttttcag attttataaa ctttacaaaa
      181 tatacttaaa attaacacat tttatttaca ttgtattaca cttaattctt atttttatca
      241 ttccacaaat aaaaaagaat aagataataa ataaaatact tatcacctta ttctttattt
      301 aattatttta caatgaaaaa tctaaactac tttttaaata gcttcctctt ccattaattt
      361 atttgtatat tactgattta attcttcaag taactaattg attgttacaa cactttatcc
      421 taaactctag aaactttata aactacatct ctagcatttg gtaaatttcc atctggaaca
      481 cctacagcaa taccaaatgg tacagaataa ccttctagta tatcaagtag ttctctgtat
      541 tttttatttg tatcaaaatt tttagagatt gggaaaaaat caatatgata agaagtatgg
      601 atttattaag tgaatacata aaaaatccta ctttgtttag atatatttta ggagttcaag
      661 aagttatgac tatggtattt atacttatac aaggctagaa ataaagatga aagaatatta
      721 caataaaaaa aaactcttta gtatatacta aagagttttt ttttattggt gccgctgatg
      781 ggactcgaac ccatatggtt tcccgtacga ttttgagtcg tatgcgtatg ccagttccgc
      841 cacagcggct tatattttaa atccgaacta cttgtatatc ctaccaggtt ttccaagaat
      901 atgcaagagg aatattgaat acaaaatact catcaaactg taaaacatta taaaaactta
      961 tgaatcaaca cagttgattt tgaatccatt gtatttacca attccaccac tcgggcatgt
     1021 catgtatatt attataacaa aaaaatatat tgtttgcaac atatttaatt ttaatatact
     1081 attattatta tgggaatatt aaataacact atattggtat ataatatata tgttacaaaa
     1141 atagataatt tttaggagtg atataatttg gaaaaaaaat taattataat agatggaaat
     1201 tcaataataa acagagcctt ttatgctctg ccagaaatga ataataaaga agggttaaag
     1261 accaatgcaa tatatggatt tacaacaatg ctatttaaaa tgatagatat atataaacca
     1321 acacacataa gtgtagcctt tgacagaaaa gcacctacat ttagacattt agaatataaa
     1381 gagtataagg ctggaagaaa gggaatgcca gatgaactgg cagaacaatt acagccatta
     1441 aaagaccttt tagataaatt caaaataaat agattggaaa tagatggcta tgaggcagat
     1501 gacattatag gaactgtttc taaaaaagca gaaaataatg ggtataaggt atacatagta
     1561 actggagaca aagatgctat tcaacttgct tcagataata caactactct tataacaaaa
     1621 aaaggagttg gagaagttga agaatacaac tttaattctg taattgaaaa atacgagatg
     1681 actccaacac aatttattga tttaaaaggt cttatgggag ataagtcaga taatatacct
     1741 ggagtacctg gaatcggtga aaaaacaggt ataaagctta taaaagagtt ttctagtata
     1801 gagaatttaa tagagaatac agataagctt aaaggtagtg taaaaaagaa aatagaggaa
     1861 aataaagaaa tagctattca aagtaaaaga cttgcaacaa ttataagaga tgtgccaata
     1921 gaagtggatt tagatagtat ggtttttggc gattatgata aagatgaact tttagataaa
     1981 tttagatact ttggatttac aagtctctta tcaagattag ttgatttggt cgattcagat
     2041 gacatagaac acgtagaaga aaaaatagaa atattaaaat taaaagatac agaaaaattt
     2101 atagatgaag taaacaaaaa aggacaagtt attttaaaaa ctgttagagg acaaggtaat
     2161 atacttgaaa aaaatataat atatattttt ataagtgtag atggagaaaa aatatattat
     2221 gtagattcaa atgaagtgtc aaagttagat agtatatttt caaataatga aattaaaaag
     2281 tatggatata atttaaaaga tgattatata tctttaaaac catataatat aaacttagaa
     2341 aatatgtatt ttgatataac aatagctgag tacttaatag attcaacttc atctacctca
     2401 tatgattgta gtgcaatagc tatgaagtat ttatcaaaaa aggttaaatc aaaagaagaa
     2461 ttattaggaa agggtgttaa atctaaaaac tatgaggatt tagaatttga ggatttagca
     2521 gagtatatgg gtcaaataat atgcacagtt aaagaaacag ttcctatgat ggaaagaagt
     2581 ctaatggata tgagtatgga tggtcttttt tatcatgtag agatgccttt agtagaagtc
     2641 ttaggtcata tggagtatga agggataaaa gtagacaaaa gtatattgga tgaattgagt
     2701 gtagagttta aagaaataat agctacacta gaaaaggaaa tttatgaatt atcaggagag
     2761 ccattcaata taaattcacc aaaacaattg ggtgttatat tatttgaaaa attagagctt
     2821 ccaataatta aaaaaactaa aactggatat tcaactaatg cagatgtttt agaaaaatta
     2881 agagataagc atccaataat agataagata actgagtata gacaaatagt aaagttaaat
     2941 tctacttacg ttgaaggact tttaaatata ataaatccag aaagtaatag aatacattca
     3001 tcatttaatc aaactataac gactacagga agaatttcat ctacagaacc aaatcttcaa
     3061 aatataccta taaaaatgga aatgggtaga aaaataagaa aagtgtttat tgcagatgat
     3121 aactgtaagt tggttgatgc cgattactca caagtagagc taagagttct tgcacacatg
     3181 agtcaggatg aaaatatgat agaagcattt aaacatcatg aagatattca taccaaaaca
     3241 gcttcacaag tttttaatgt tcctatggaa gatgttacta gtgaattgag aggtgcagct
     3301 aaagctgtaa actttggtat tatatatggt aaaagtgact ttggtctagc tgatgattta
     3361 aatatacctg ttccaaaggc gaaagaatat atcgaaagtt attttgctaa ataccataaa
     3421 ataaaagagt ttatggacac aacagtagaa aaggctagtg aggatggata tgctgtaaca
     3481 attttaaaca gaagaagata tataccggaa ataaagtcaa gcaattttat ggaaagaaat
     3541 agaggaaaga gatttgcaat gaatgcacca atacaaggaa gtgcagcaga tataataaaa
     3601 attgctatga taaatgttca taaaaaattg gaagagaaca atttgaaatc aaaacttata
     3661 ctacaagttc acgacgaatt aatagttgaa gcaattgatg atgaaataga tatagttaag
     3721 aaaatagtaa aagaagaaat ggaaaatgcc gtaaatatgg atgttcactt agatgtagat
     3781 ttaaatgtag gtagttcttg gtatgagaca aagtaggtga ttttatgttg atattaggac
     3841 ttacaggagg gataggctgt ggaaaaagca gcctatcaaa tatatttaga aatcttaaca
     3901 tttctatagt ggatgctgat attatatcca ggaagatatt tgaggataag ttgttattag
     3961 aaaaagtatt tgtccatttt gggcaaagta taaaaaatga tgatggaact ctaaatagaa
     4021 aagctcttgg gaaaatagtt tttagtgatg aagaaaaact taaggagtta aataatttga
     4081 ctcatccacg aataagagaa aaaataataa gtgaaataga agaattgaga aaaaaaggag
     4141 aaaatatagt agtattagat gcagcaatat tggtagaaag tggtttttta gatatggttg
     4201 acaagctatt agtagttact tgcaaacaag aagtacaaat tagtagaata caaaagagag
     4261 ataattgtag taagcaggaa gcacttagta ggataaattc acagatgagt caggaagaaa
     4321 aatccaaata tggtgattat ataatagata attcgggaac aataactgaa ttagaaagta
     4381 aagcacataa atttattgag tacatgaagg agaattggcg tgaatagtaa gaaagtattg
     4441 attctatcta tttttataat cttatttgga gcactattaa tggaaagcaa agtaatacat
     4501 aaagttttat atcctaaaaa atattcagag tatgtagaaa agtattcaaa agaatttaat
     4561 ttagatgaaa atatagttta cagtgttatt aaagccgaaa gtaagtttaa tagttctgct
     4621 gtttcaaaaa aggaagcaaa aggattaatg caaatattag acataactag agattgggga
     4681 gcagaggaac taaatttaaa aaatgtagat attttcgacc cagagactaa tataagactt
     4741 ggatgttggt atttaagtaa gttatacaaa gaatttggta aattagattt agtgatagct
     4801 gcatataatg gtggttcagg taatgtgaaa aaatggttag aaaataatga atatagtaaa
     4861 gatggcgaaa atctacatga tatacctttt aagcaaactt caaaatatgt agaaaaagta
     4921 aaaaataatt acgaacatta taataagata tatggcaaga aaggaaaaaa ctaatgaaga
     4981 gaataaaagt cttaacagtc gttttagcta taactcttat ggtagctggt tgtagtaata
     5041 ctaaaacaaa acaaagtagt agtgattcaa gtttacaaag tcaaagttct atagatgcta
     5101 agtatataaa cttaacaatg gtaactccaa aaactataaa tcctataact aatactgata
     5161 agtctgttgg ttatataatg aatttagttt atgatagttt atttactata gatgagaact
     5221 acaatgtaat tcctcaatta gttaaggagt ataatatagc tcaggatgga atgagtatag
     5281 atataaagct gaaagatgca aagtggcatg atggaaaaaa tgttacttca aatgatgtaa
     5341 actatactat tggtttaatt caaaaaagtg cagatagtcc atataatgaa tttactaaaa
     5401 atatagcttc tgtgaatatt aaatcagata aagattttac aataaaattt aaagctagat
     5461 atgctttttc aattgatagt ttaatttttc caatcgtatc tcaaaatcat ttagattcaa
     5521 aggatgtaaa tgacaagaat aataatatga taggaaatgg gaaatataaa atagaatcat
     5581 atacagaaag agaaggaatg gttttatctg taaacaaaga ttactatgaa gaagttccaa
     5641 agaccatgaa gaacataaaa gtaggtatgg tgcctaatga agaggcaagg acatctatgg
     5701 ttatggctct tgatagtgat ataaccaatg ttacattaaa tgatttaagc aagtttcaag
     5761 aaaaagagtt taatataaca agatatcaag gaagagatta tgagtgtgta ttatttaatt
     5821 ataataatcc attttttaaa gatgttaatt ttagaaaagc aattgctcac tctatagata
     5881 aagatagaat tattagtgaa ggttatatgg atgatgctac tccagttaat tttccactta
     5941 attcaaaatc aaaatactat aatagtgaaa tgaaggattt agagtttaat aaagataagg
     6001 cgatagagtg tcttgctaag gtagaatatg caaatataaa ctcagtaaat caaaatgaca
     6061 ataaagtaaa aaatcagaaa aaatcagcta aaaagaactc aaagaagctt acgccagagg
     6121 aagaagcaaa agctaaaaaa gcagaagaag atagaatcaa aaaagaagct gaagagaaaa
     6181 aaaatagaga aaaagaggaa gtaaagaaaa gtctttctga aattaactta aagataatag
     6241 taaatagaga taatagtgag aggataaaaa ctgcaaacat aataaatgaa aacttaaaag
     6301 ctataggaat aaatagtaca gtaaatcagc tgtcagataa ggatatggaa aatgctctta
     6361 attctaaaaa ttatgattta gcattagttg gatggaaact atcaattata ccagatgcaa
     6421 gtagtattat agcaagtagt ggatatactg atgataagtt aaatggatat atgtctgcac
     6481 ttacaagttc tacgtctgaa attgaaacaa aaaaagcata caaagatgta cagacatata
     6541 taaaagataa tgtaacattt ataagtttag ctgtcagaaa caactatatt gtaagtaata
     6601 gtagattaaa aggtgaaatt actccaaatg attttgatgt atatgaggga atatctaatt
     6661 tagatataaa atcgaatgaa tcaaattaaa tgattaattt ctattcaaaa agtacaaact
     6721 atatgataaa atacccttat agggtataag gaggtaatta aatgaagaag aaactattag
     6781 tagaaggtat gagttgtggt cattgtgtaa accatcttaa aactgcatta acagaagata
     6841 tagatggtat tgaagtttta gatgtagatt tagaaaataa gtgtgcatct gtagatatga
     6901 aagatgatgt atctattgaa aaattaaaag aagtgatagc agaattagga tttgaactaa
     6961 agggaattga atagaaatta ataaactaaa aaaagacctc tattggggga gtaatagagg
     7021 tctaaaaatg ggggagattg agtatgtttg attttacatt actattatct ccccttttaa
     7081 attaatatat acataaaaag taaaaaaatt ttgtaatgac tcacatttct tgagcttttt
     7141 gaatatacta taaatagcta agggagggag tatcatggaa tctaggggat ataaagagga
     7201 tttaaaaaaa atgatacttg atatggccca caaagaactt gagagttatg tatcacaaaa
     7261 aggagcaaaa aaatactttg aaggtaactt ggataagatt attaataata atataaaaaa
     7321 attcaatcaa agtattggtc ttaatagagt atatttaaaa atgttaaaaa agtgtgcata
     7381 tcaagaaagt gtaacttcaa tatcaaggac tataaagaaa gaagcattat ttaaatcaaa
     7441 aaaagagcta tttgcttttt cagagtactt aaaacttgat attaacaagc gactttcata
     7501 taatcaaatt ttaagaaaaa tatcaagata tatatactac aatagaagtt catatgctca
     7561 aaaatacgtt gttttcaaaa gaggagatga agaatatctt ttagaaccag agaaaataaa
     7621 agatgaatta atatcaagct ataggtcaaa gactagagaa gatatgaggt caatagcaaa
     7681 acttttggat atagaagtag aagatgatta taatgctgaa gatattagaa aaaaagtaat
     7741 aaattatatt atcaaagaaa aacttaaaag ttagagtttt aaagaataag ttctgccaat
     7801 ttgcatgatt aagtataact tgggtataaa ttttttgtgc atttgaaaga gcttattctt
     7861 tttttgacat attcttttaa ataatttacc accatttggt aattggtcat catcattcaa
     7921 atttttaact taatatgatt taaataaatt tataataatg atataatata aaaaaaataa
     7981 aaattggaga gtgatttcaa aatgaagttt aatttttgcc ataacaattt gaatgttact
     8041 aacttagaaa aaagtttaga tttttataaa aaagctttag ggcttaaaga ggtaaagcgt
     8101 aaggaagcag aagatggaag ttttatatta gtttatttag gagatggtat tactagtcat
     8161 acattagaat taacttggct tagagattgg gacagaccat ataatttagg agacaatgaa
     8221 tttcatctgg ctttagaagt tgaggagttt gatgaggcaa aaaaacttca taaagattta
     8281 gattgcatat gttttgaaaa tgaaagtatg ggaatttatt ttatagcgga cccagataat
     8341 tattggattg aaatacttcc taaaaatcat taattttaag gatatgttta taatttcttt
     8401 acaattaata agagaataag ttatcattaa attgtaggga ctaaaaatgg ataataatgt
     8461 tatgaattta tgactataaa aatttgttgg ggggagtgat agtagtgaag aaagctatag
     8521 cagctttagg aattggggca gtagcagtat ctgttagctc cataaatgca agtgctcttg
     8581 aaaagggaac agtaacagca agtgccttga atataagaag tggaccaagt tctgattgcg
     8641 ataaggtagc aaaattatac aagggaaaaa ctgtagagat tttagaaaag tctaatggat
     8701 ggtataaagt aagggtatcg agttctgttg ttggatgggg aagcgcaaaa tacatatcaa
     8761 caagtggttc atctgaaggt acatcaaatc aaaataatcc aacttcaagt gggacaacta
     8821 ttagtggaaa tggtaaagta aatgtgagtt ctagactgaa tgtaagaagt ggtgcaggta
     8881 ctaactattc attagttggt aaagcaaata atggagatgt agtaaaatta ctagaacaaa
     8941 gcaatggatg gtataaaata aaactatcaa atggagttac tggatgggct agtagccaat
     9001 atatctctaa aacatcagaa gatgttggaa cgaataattc cagtaattca aatagtacta
     9061 ataattctga taaaaaacca tctagcgagg aatcaattga aggcaagaat ggtaaggtaa
     9121 catctgctgt gagtttaaat gtaagaagtg gtcctgggac aagctattct ataataggaa
     9181 aattaaatgg tggagatgtt gttgaattaa aagctaaaaa taatggttgg tataaggtaa
     9241 agttatcgag tggaacaatt ggatgggtga gtgccagtta tatttctgag actagtgaag
     9301 gtacaaaaga aaaaccaaat tcatcatcta accaaaattc tcaatctaat agtaactcaa
     9361 agccatcatt tactggaaat tcagataaat ctacagcaaa aggttcaaca atcgtagatt
     9421 ttgcatatac tcttattgga ataccatatc aatggggagc ttcaggacct gataaatttg
     9481 actgttctgg atttacacag tatgttttta aacattctgt aggcgtttct attcctagag
     9541 tttctagaga acaggctaat tttggaagtg caatttctat gggaaattat gcaccaggag
     9601 atttagttta ttttgacact gatggtgatg gaacaactaa tcatgtaggt atatatgtag
     9661 gaaatagtaa atttatccat tgtagtggta ctcagactaa tcctaataag gttaaagtgg
     9721 ataatttaac tagttcatat tggtctaaag tattacttgg tgcaagaaga tttgtttagt
     9781 aagtacaatt tcttattaaa aaattagaag aaataattat ttatattcat aaattgtgac
     9841 tcttaaaaat atactttatt aataatataa ttttgggagg gacaaactaa tgaatagaag
     9901 aaaaacgata tacagaggat ttaataataa tagacataag attgatttac gtaaatatgt
     9961 tataacgatt gcttgcttgt gtctaattgg ctattattct tatacaaaaa taaaagatag
    10021 caagatattg gagtatgtat cggctaaaat accatttcta aataattcat cagatataac
    10081 ttataaagat atatctgatg aattaaattc aataaaaaaa gggaaaaaat ctaaatcaca
    10141 aaccaattca gatgataaac aagaaactaa tccagaaaaa gcggtaaata acactaaaga
    10201 gccagaagaa gtcaaattag ctactataga aggctgggat atgtacacta ttcaagttgc
    10261 tgcaatagat aataatgatg atttgaaaaa aatacagaca tcattagtta ataatgacat
    10321 acctttttca gttatggaga aggatggagt aaaaaaaata cagacctatt cctcttttga
    10381 tgaaaatgat gttagaaagc agataagcag tgtaagaaag gtgtttccag atgcattttt
    10441 atcacactta gatgcaccta tgttgtcttt agagtataca agtaactacg cttatataga
    10501 gagtatatct aaagaattaa ataaattaat aacaaacttt aaagaagaat caagtttttg
    10561 gtcgaatgct gaaaataatg tggatatgga aaaatataat actattttga caaatagaaa
    10621 ggcaatatca caaaatatat caaaagaagc tgaaaagatt gattattctg aaatgcgttt
    10681 atttaaagat aatttaatgg agtatgtaaa gaatgtgaat gaaaaaattg atacagcttc
    10741 aaaagcagca aatgaagaaa aatatagtgt aagtaaaagt ctgcttttat catccatgca
    10801 ggagtactct atgtttataa attctataaa ataaaataca gaaaattttg actttagaaa
    10861 gctatatgaa aaggattgtt taattaagca atccttttcc taaatttgac ttaatataga
    10921 ggtgttgatt tataaaataa attataaaaa tacggatatt tttttaacaa ttgttaagat
    10981 tttttaatat atatagtata cttatagaag gcagaaaaaa tgttttttta tagataaggg
    11041 ggtaaaacaa tgaaactctt aacttttaag ggagggatac atcctccata tagaaaagag
    11101 tactctaaca caaaggcact tgaaaaagct caagctccaa agatagttta cattcctctt
    11161 cagcaacata taggggcacc agctaaacct attgttgaag ttggtgatga agtaaagttt
    11221 gggcaaaaaa taggtgaaca acaaggtttt gtttcatgta acgttcattc ttcagtatca
    11281 ggtaaagtta ttgctattga acaacatgaa gtaccaggtg gttcagctca atgtgtagtt
    11341 atcgaaaacg actttaagga agaattacac gagagtgttc aaccaaaagg tcagttagag
    11401 gatttgagca aagaagatat agtaggtatc ataaaagagg caggaatagt aggtatgggt
    11461 ggagctacat tccctaacca tgtaaaagta tctcctccac cagatagcaa ggcagaagta
    11521 gttatattaa atggtgcaga atgtgaacca tacttaactg cagaccacag attaatggtt
    11581 gaaaatccag aagatgttgt atttggttta agagcattaa tgaaggtatt agatgtcaaa
    11641 aaaggattta taggaattga aacgaataaa ccagatgcta tagaagcaat tcaaaatgta
    11701 gctaaagact atagcgaaat cgaagttgtt ggccttcaag tgaaatatcc acaaggagca
    11761 gagaaacaac ttatttatgc gtgtacaggc aaagaagttc catctggtgg attaccaata
    11821 gcagcaggtg cagttgttga caacgttgcc acagcagcac agatagctaa gtctattaaa
    11881 acaggtatgc ctttggtaga aagaatcact acaataacag gtagttgcat caaggagcca
    11941 aagaacctaa taacaaaagt tggaacatta gtttcagaaa taatagaaca atgtggtgga
    12001 tttaaagaag ataagaagat tggtaaggtt ataatgggtg gacctatgat gggaatagct
    12061 caatatacta ctgaaatagc gactaacaaa ggttcttcag gaattttatg tttggatgag
    12121 gaagaatcac gtactccaga tattcaaaac tgtttaagat gtggaagatg tacagatgta
    12181 tgtccatcat tcttacaacc acttttcata agtgcatatt ctttaaaaga tgactatgac
    12241 acagctgaat atcatagagc aatggattgt atagaatgtg ggtcttgctc atttatttgt
    12301 ccagcaagaa gaccattact tcaatctata agatcagcaa agagagaaat aggagcaaag
    12361 agaagaaagc aagctgctca gaaataaaga aagttaaggg ggaaaaacga tggaaaataa
    12421 gttgatagta tcatcttctc ctcatgtgag aagtaatgaa gatacttcat atataatgaa
    12481 acaagttatt atagcactcc ttccagcagc agtagcagga gtatacttct ttagacttaa
    12541 tgctttgagt gctatgtttt tttgtatact tggtacagta ggtactgaat ttttatatca
    12601 aaaatttacg aagcaaaaaa gtactatagg agatttttca gctgttgtaa caggattatt
    12661 attagcattt aacgtaccag catcacttcc ttggtggatg tgtctagtag gaggaatatt
    12721 tgcaatatta gtagttaaaa tggtatttgg tggaattgga tgtaactttg tcaatccagc
    12781 acttgctgca agagcatttt tattagcatc attcccagta gcaatgactg cttggactca
    12841 accaggtgtt aactggatag gtaaaaattt agatgcagtt actacagcaa ctccattaag
    12901 ctttttgaaa aatggagcag caggactagc agacctttct agtaatggaa ttagtcttgc
    12961 tgacatgatg atagggaata ttggtggatg tataggtgag acatcagcaa tattattatt
    13021 attaggtgga gtatacctaa tgtataaagg cataataaat tatgttatac cagtatttta
    13081 catagcaact gtatttatat taacattcct tttaggtgga tttaatataa ctttcgcaat
    13141 atatcaactg tttgctggag gacttatgtt aggtggattc ttcatgctta cggattatac
    13201 aacttcgcct atgactaaaa aaggtcaaat aatatacgct gtattagctg gtcttataac
    13261 aactgttata agaatgtatg gtggatatcc agaaggtgta tcttactcaa tactattagt
    13321 gaactgtctt gctccactta tagataagtt tgttagaaat agagtatttg gggaggtggc
    13381 taaataatga acagtatggt gagattaggt ggtactttac ttgctataag tgcaatagct
    13441 gcattagctc ttggagctac taatcaagtt acagctccag taatagaaca aagaaatata
    13501 caagcaaata atgaacttag aaaagcagtc cttccagaag caaaagaatt taaagaaatg
    13561 gataaaagta catataaaga ccttggagat ggtcttatag ctgaagtgta tgaaggttta
    13621 gatggttctg aagtggttgg atatacttta aaagctaaac caagtggata tggtggagaa
    13681 attgaagtta tggtaggtat atcatctgaa ggtcaagtta caggtgttga cattggtaat
    13741 atgtcagaaa cagcaggtct tggagcaaaa gctaaggatg atgcatttaa gggtcaatac
    13801 aaaggaaaaa ctgctgaacc tcttgaagta gcaaaaggaa gtacaacagc agataatcaa
    13861 atattagcaa tatcaggtgc aactattaca tctacagcag ttactacagg agtaaatgct
    13921 gctatagatg ttttcaatag tgctcttaat aaataaggag ggttaattat gaatttagct
    13981 aaagtattta aaaatgggct aatagatgag aacccaacat ttgttcaagt tataggtatg
    14041 tgtccaacac tagccgttac aacttcagca ataaatggaa ttggtatggg tctttcaaca
    14101 gcagcagttt taatatgtgc aaatttagtt atatctttaa taagaaaaat tacaccagat
    14161 aagataagaa tacctatatt tatagtagtt atagctacat ttgttacaat agtaggtatg
    14221 cttttaaaag catatgttcc tgctcttgat aaggctcttg gtatatatat accattaata
    14281 gttgttaact gtttaatatt agctcgtgca gaaagttttg ctttcaaaac aggagctatg
    14341 ccttcaatag ttgatggtgt aggtcaaggt cttggattta ctgttgcact tacaataata
    14401 ggtgcagtaa gagaattact tggaaatggt agtttatttg gaatgacatt atttggagca
    14461 tctttccaac cagtattaat atttatatta ccaccaggtg cattcttaac attaggattc
    14521 ttatttgcag gatttaataa attaagaagt aaaaaagcat agtggaggtg taataaatga
    14581 atttaatact tttattttta agtatcgttc ttgttaataa cgttataaca tctcaattct
    14641 tgggtatatg tcctttctta ggagtttcta agaaggttga tacagcagta gggatgggag
    14701 ttgcagttac attcgtttta actctagctt caattataac ttattttata cagattttat
    14761 tagtaaaaac tggaactgga ttcttacaaa caatagcttt tatattggtt atagcttcta
    14821 tagttcagtt cgttgaaatg gtaattcaaa agatgagtcc atctttatat caagcattag
    14881 gagtttattt acctcttata actacaaact gtgcagttct tggtatagct cttgttaatg
    14941 ttcaaaaaag ttataattta gtagaaacaa taataaacgg atttggagcc ggagctggat
    15001 ttactttagc aatagtaata ttcgcaggta taagagaaag actagaatta gctgatatac
    15061 cagaagcatt taaaggtttc cctataacgc ttatatcagc aggtttaatg tcaatagctt
    15121 tcttaggatt tacaggactt ataaagctat aaaaaagagg ggtgaaaatg gtgatactta
    15181 cagctgtatt agttttagga attatgggct taatcttcgg gatagtacta gattttgcat
    15241 ctaaaaagtt tgctgtagaa gtagacgaga gagtggaagc tatactagga gtacttccag
    15301 gagccaactg tggaggttgt ggatttccag gttgtggagg tcttgcaaat gcaatagttg
    15361 aaggcaatgc accagttaat ggttgccctg taggtggagc agatgttggt gcaaaagttg
    15421 gagaaataat gggaataagt gctgaagcag gtgagaaaca agtagcaaaa gttatttgta
    15481 aaggaacttg ttcaagtgca aaagataaat atgagtatga aggaatatca gattgtagag
    15541 cagcaaatgt tttaaactca ggtgctaaga tgtgtaaatt tggatgttta ggtcttggaa
    15601 catgtaaaga tgcttgtaaa tttgatgcta tttctatagt tgatggaata gcagtaatag
    15661 atgaagaaaa gtgtgtaaat tgtggaaaat gtaaagaagt ttgtcctaag ggtataataa
    15721 tcacaaaacc agaaagtcaa gaagtagttg tagagtgtaa tagcaaagag tttggtaaag
    15781 ctgttaagga aaaatgtact gctggatgta ttggctgtgg aatgtgtgta aaagcttgta
    15841 aatttgatgc aataatattt gaagacaaaa tagctaagat agaccctaat aagtgtgtag
    15901 gttgtatgca atgtgtagca aaatgcccta caaaagtaat atcaggtgac ataactaaga
    15961 aaaagaaagt tactattgac caagaacttt gtgttggatg tacagtgtgc aaaaaacaat
    16021 gtaagtttga tgcaatagag ggagaattaa aagaaaaaca taaggtagat gcagataaat
    16081 gtgtaggatg ccatttatgt atggaaaaat gtccaaagaa agctattaaa attttgtaat
    16141 atggacaaaa taaggatatg gaataatttc catatctcta atgagtacat aaaacagagg
    16201 tatggaataa tttccatacc tctattttat gtatatttat tctaatggta ataaatataa
    16261 caatagtgta acaaaaaagg ttcgacattt ctgttgaatt ttttacaata tagtgataga
    16321 ctaaataggt acaaattacg tacaaatgag gtgaataaat gaatataatc ttggcatcag
    16381 cctctccaag aaggaaagaa attctagaga acacgaatgt aagatttgat ataattaaaa
    16441 atgaaattga tgagattata ttggaaggtg aaacaccaaa acatttagtt atgagattag
    16501 cttttgaaaa aagtatgtct gtagcatctg aacataatga agatgttgtt attggagctg
    16561 atacagttgt tgttttagac aatgctattt taggaaaacc aaaagatgag tcttgtgcaa
    16621 gagatatgtt aaaaagacta tctggaagag agcatcaagt cataacaggt attagcttaa
    16681 taaacttgtg cgaagataaa aaggtaattg actatgtaat cagcaatgtg aaatttaaaa
    16741 cgttatcaga gcaggatatt gaggattatc tgaaaactaa tgagtcattt gacaaagcag
    16801 gagcctatgg tatacaaggc tatggagctt tgttagttga agaaattaga ggcgattatt
    16861 ttaatatagt tggtcttcca atttcaaggt taggcgacct attaaaaaaa tattttagta
    16921 taaatctttt ttatggagtg tgatttatct gaaaaagtca tttaattcaa ccattaaggt
    16981 aaaagaaatg gcaccagagg aaagacccag agaaaagatg cttgctaaag gagtaaaaag
    17041 cttatcaaat gctgagttgt tggctatact tttaagaact ggcaacaaga ataaaaatgc
    17101 tattgaatta gccaactata taataaatag agatattcag ggtattagac atttagaaga
    17161 tatgacaata gaggagctgt gtaatataga tggaattggg ttatcaaaat caacccaaat
    17221 taaagcagct ttagaactag gctctagagt agctagtttt aaacctataa agtataaaat
    17281 aaggaatcct tgggatatac agaggtatta tatggatagc ctaaggtatt tgaaaaaaga
    17341 ggtttttaaa gcggttcttt taaacacaaa aaatgagata atatctgatg tagatgtatc
    17401 tatagggact ttaagctcgt cattagtcca tccaagagag gtttttaaag aagctataag
    17461 aagaagtgca agtaagataa tagtgatgca taatcaccct tcaggaagtg tagaaccatc
    17521 aagagaagat aaaaatatca catcaagact tattaaatgt ggagaaataa ttggaataga
    17581 aataatagac catataatta ttggagatgg attatacttt agctttaaag aaaatatgat
    17641 aatttgactt tatatgtgat aaaataatag gagaatgttt taaacatgat tcttaatagt
    17701 aggaaggagt aaaatcatgg ctaaggaaaa aaagaaagaa aaaaaaggtt tttttagttt
    17761 taataagatg acaaaggata tgggaataga tttagggact gcaaatacat tagtttacat
    17821 aaaaggacaa ggtattgttg ttagagaacc atcagttgtc gcaataagag atgatagtaa
    17881 agaggtatta gcagtaggtg aagaagcaaa aaaaatgata ggtagaacac caggaaatat
    17941 agtagctata agacctatga aagatggagt aatagctgat tttgacataa ctcaatcaat
    18001 gattagctat tttatacaaa aagcggctga taaaaaaggt gttgtaagtc caagaatagc
    18061 tatatgtgtt ccttttggag ttacagaagt agaaaagaga gctatagaag aggctgcaag
    18121 acaagcagga gctaaagatg cattccttat agaagagcca atggcagcgg ctataggggc
    18181 tggattaaaa gttgaagaac cagaaggtaa tatggtagtt gatattggtg gaggtacttc
    18241 agaaatagct gtaatatctt taggaggaat cgttactgct aaatctataa gaataggtgg
    18301 agacgaattt gacgagtcta tagtggctta tgtaaaaaaa gaatataacc ttatgatagg
    18361 tgaaagaact gctgaaaatg taaaaattaa cataggttct acatttaaag atgatgaaga
    18421 gataaatatg caaataagag gtagagattt aatctcagga ttacctaaaa caatagaaat
    18481 atgctctaca gaagttagag aagcattaaa agaacctgta agttcaatag tagatgcaat
    18541 aaaatctaca ctagaaagaa ctccaccaga attagcctca gatataatgg aaaacggaat
    18601 aatgctaaca ggaggaggag ctttgcttag aggtcttgat aagcttatta cacaagaaac
    18661 tggtatgtct gtacaaatag cagaaacacc tcttgattgt gttgcacttg gaacagggaa
    18721 atctgttgaa gaccaagaaa tctttgaaaa agtattaatg atgaatacta agaattagaa
    18781 gttggtgatg gccttgagat ttgacaagaa caaaaatgag aaaaaaagga ttaatgttaa
    18841 agtgatagca acgggcgtag ttgctatcac tttaattgga attgtgggta tatcaattgg
    18901 taaattttct agtggtagtc caataaattt gggagtagct tcagatgcta ttacaagtgt
    18961 aggtaaggga ataaatgatg gattttcttt tatcaaaaat ggttttaaag atgtagcaaa
    19021 ttttaaagat aattctaaaa aggtaaaaaa attagaagaa gaaaatgaaa aacttaaaaa
    19081 agatatgata gctttaaatg caaaactaga taaaacagaa tcgctagaag agttaaaaaa
    19141 gacactgaac tttgtacaag aagagtataa ggcgacaagt atttcagcaa gtgttgttgg
    19201 taaaaatgat ggaaactggt atgagagttt tgtcataggt gcaggaaaaa attcaggtgt
    19261 aaaaaaagaa agtatagtta tgaatggtag tgggttagtt ggcatagttt atgaagtttc
    19321 caacaattat agtaaggcaa tttcactatt ggattctaag gcatctgtaa gctttaaact
    19381 agcaaaggat gccaatgcta aaggtacaat tactcaaaat actactttag ataataaaga
    19441 tagttataat agtaaaggat atttgcaagg atatatgttt gactcatcat ataatgtaat
    19501 tcaaggagat ataataacaa catcaggtct tggattcttc ccagatggta taccaattgg
    19561 cgaagttgaa aaagtggttg atgataaaga taagtcatta aaatatgtag ttgtaaaacc
    19621 ttatgttgat tttaagaata taaatgacgt tgtagtcata gaaccaagaa atataggata
    19681 aaggggataa aaacaatgaa aaaagtttta ctttgtctgt tggggattgt gcttatgact
    19741 atagaaaata gtataacaaa ttatatagat atttttggca taagctttaa tttagttctt
    19801 atatatgtaa ccataatctc tctttattta gatgaattag aaggtgggat tatagcagca
    19861 ataatagggc ttattaaaga cataaccgtt ggtggaattt ttggtgtaaa tgcattaatt
    19921 ttgtttatta ttgcatatgc aattggatac atgagagatc agctatataa aggtagttat
    19981 attattactt tcgttttaat ttttataggc agtttaattg attcaattgt caatataggt
    20041 acatctagta taatttatca aagccacaac atatcaacac tttttgtaaa aggtctacta
    20101 gtagcaccaa tagtgaatag tttaattggt ttagtaatat atcgcttatc taaaaaagca
    20161 gttttaaaac ttaaagaaga ttagttggtg atttcatgga agaaaataaa caagttgata
    20221 gactaaaaat aattaagata gtcatgagcg tgatattttt agctatttta gtaaaaataa
    20281 tctatatgac tacatttaaa tatgaatatt acaatgagtt agctgagaat aaaacatata
    20341 aaaagttggc aatagaagct ccaagaggag aaataaaaga tagatatgga agactgttgg
    20401 caggaaataa aaacttattt actgttcaag tttcaggtaa cgatattaat aaaaaagatg
    20461 caaataagca ttcaagagca aatgaaatat cattaaaact tataaatttg ctggagagaa
    20521 atggtgaaga atatgtagat gagtttccta tatatgtaga aaatggaaag tactattata
    20581 catatgacag agatattagg gaatataaat cagaaaatgg tattccaaat gattataatg
    20641 ctaaggaaag tttttattat ttagtagata aacttatatc agctggaata ttatctcaag
    20701 aagataaaag attggatgcg acaagattac aggcaaaact aaatgaaaat ggatattatc
    20761 cacctatttt agttagtaaa tggatgttca cagcagaaag agataagaga gattggttgg
    20821 ctagttataa gataaaagaa actaaactaa gtgccaaaga agcgtttgaa aaagtaagaa
    20881 atagtgatgc attggaaatt gataaaagtt taagtgatga agatgcaaga aaaataatgg
    20941 tcgtaaggga tttgataaaa tcaaaaggat attctcaata taatccagta actattgcta
    21001 aggatgtagg agaaactacg attgctcaaa ttgaagagcg tgctatggac cttgttggtg
    21061 tttcaatagc agtagaacca gtaagatatt atcctaatgg gtcattagct tctcatatgt
    21121 taggatatgt aggaaaaatg ccatccactc aaatagaatc ataccttcaa aaaggttatg
    21181 aaactggaga tatggtaggt cttgcaggag tagagaagag taatgaaagt agattaagag
    21241 gaactgatgg ttataaaatg gttaaagttg atgcattggg tagaatcagt aaagaaattg
    21301 aatctaaaaa acctaaatca ggagatactg tgtatcttac actagataaa gatttacaag
    21361 aagtttcaga taatgcatta aaacagataa tcgaagttgc ctctaaagga ggtactttta
    21421 aaagtaagtt tggggataaa ccaattagtg catatgcagg aaaagcacaa tcagcagctc
    21481 ttatagctat tgatgttaaa aatggagaag tattagcttc atcaagttat cctaattatg
    21541 acccaaataa atttgcaaaa ggtatctcta cagaggatta taaagcactt caaccaaaaa
    21601 atccaaatga cttactagca ggaagtccac ttttaaactt ggttacacaa ggggaatttc
    21661 agccaggttc ttcatttaag atggtaactt caatggcagc gttagaaaat ggtctggatc
    21721 caaactttac tataaatgac cctggagtta taatgttagg taaaaaatct tttggtgact
    21781 atgtatggaa ccatggtaga ggaaaccatg gtatgacaaa cttatataaa gcaatacaag
    21841 agtcttgtaa tatatatatg gcaacaattg gtacaggtaa aacatggcca ggtggaaaaa
    21901 gcattggtat agatatgaat gcaaataaaa tacttgagta tgcaaaatta tttgggcttg
    21961 accaaaatac agggcttcaa gatgaagtag aagaaagagc aggtaaagtt cctagcacag
    22021 aagataaatt gaaatcaaca caagctcttc taaagtcaaa tttagaaaga gaaatggcaa
    22081 atgattttgt tgatataact agagaaaaaa atcctaaaga atatgagaaa agaatcaatg
    22141 agattgtaag ctgggcagca gaaaagaaaa ctcctggtag agtcgaaaca atgaataggc
    22201 taaagaaaat gaatgttaaa gaagatagaa tagaagatgt agcagattta gcagtattca
    22261 gttattttaa ttttgcaaaa tggagtactg cagatacatt caacttagct ataggtcaag
    22321 gtgagaatgc ttatacacca gctcaaatat caagacatgt agctgctata gcaaatggag
    22381 gtaatttagt agaattatct gtagttgata gagctgtttc aagtgattat tcatcagtta
    22441 aaattaatga ccagaagaaa gtagaaaaaa taccttttaa aaatccagat aacttaaaag
    22501 aattaacaaa aggtatgaaa ttggttgcaa gacaaggtac agcgaagagt gcttttgctg
    22561 attttccaat agatgtagca gcaaaaactg gtacagctga gaaaagtggt aaaataccta
    22621 cagataatga gtatgaatat ttaaaaagtc atatgtcatc ttataatgtc aacctaaatg
    22681 atgcgattaa actagctgat aagatgaaag cagaaaaaga aaaagagctt tcattagcta
    22741 aggaaaagga aataaaaaag aaattagaaa ataaagattt gaaagacgaa gaaagaaaga
    22801 aattagagga agaattagaa gatggagtaa aagttagatt agaggacact gataaggtga
    22861 attcttcata tctaagaaaa gctataaaag aattaaatcc aaaaataaca gatgaccaaa
    22921 tagatagatt taaacaagat tatggttctt ttacatggac tgttgctttt gctccagcag
    22981 atgacccaga aatagctgtt gtatgtgtaa ttcctcaagg tgattcaagt gtattctcac
    23041 ttttaccaac tagagaagtt attggaactt atatgggatt agagccaact aatagtaaaa
    23101 atgacaacaa gacgaatgat gttaacaatt cttctgacga aaatataaat ttcgagtcac
    23161 aaattaatag atagctcaat ctatgtataa actttagctt tatatagaaa aaataaaagg
    23221 gtttaaagcg tatttgaaga atataaatga tatatcactc tggaggttgt tatgtcttta
    23281 agagaaattt gtagtcaaga actagtagag ttcaagggta ataaaagagg gataatagta
    23341 aatataaaaa gagaagctcc atttgaggaa atacaggaaa aaataataaa caaattggaa
    23401 gcatatgttg gcttttttaa tggggctaaa attagtaaaa ttaatagtga ttgtttaact
    23461 gacatggaga ttctagaact gaaagaagga attacttcta gatttgatgt ggaatttgtt
    23521 gaagatcaaa aaattgaaga aaatagtaat tttccaacaa aatatgtcaa taccctaaga
    23581 tctggtgaaa atattgaatt tgaaggtgat gtagttatat tgaatgatat gaaacctggt
    23641 tcaaaagtcc tttcaaaatc aaatacagtt gttatgggag atataaatgc tggtgctaag
    23701 gttgtagcag gaggtaatgt ttttgtaatg ggtaaaatag aaggttttgt acatgcaggt
    23761 gcagaaggca atgaatttgc atatgttgtt gctggaaatc ttaatcctaa aatattacaa
    23821 atagcagata atattgcaga agctccagat gatgaagaaa attatgaaag tgaatcagaa
    23881 attagcccag aaatagcctt tgtaagtaat ggaagaattg taatcgaaag ctatttgtca
    23941 aagttagaca aatagaaaga aaaatagggg gtagtattat gagcgaagtt atagttataa
    24001 catctggtaa aggtggggtt ggaaaaacta ctaccgcagc aaacttagga actgcgctaa
    24061 gcctagaaaa taaaaaaaca gtagttgtgg atgcagacat tggacttaga aacttagatg
    24121 tagtaatggg tcttgaaaat cgaatagttt atgacatagt agatgtagtt gaaggaactt
    24181 gtaggcttaa gcaagctcta ataaaagaca aaagatttga taatctatat ttattaccag
    24241 cagcacaaac tagagataaa aatgctgtct cagttgagca gatgattgac ctgtgtgaga
    24301 aactaaaaga atctttcgaa tatataataa tagactgtcc agcaggtatt gagcaaggtt
    24361 ttaaaaatgc agtagctgga gcagatagag ctattgtagt tactaatcca gaaatatcag
    24421 cagtaagaga tgctgataga ataataggtc tattagaagc aaatgaaata aaagagataa
    24481 gattagttat aaatagaatt agaaatgata tggttaagcg tggagacatg atggataaac
    24541 aagatataat agaaatatta gcaatagatt tgttaggtct tgttcctgat gatgaaagca
    24601 taattatatc aacaaataaa ggagagccgg ctatacttga ttctaagtca cttgctggtc
    24661 aagcatacaa aaatatcgcc aaaaggatac taaatgaaga agttcctcta cttgatcttg
    24721 aagttgaaga tggattcttt ggtagactta aaaaaatgtt tagcatggct aagtagcaaa
    24781 ggaggataca tctgtgttag atttatttag agttttttct aacgaggcta aaactagtaa
    24841 gtctgttgct aaagagagat taaagctagt tttagttcat gatagagtag attgttcacc
    24901 acaactttta gagatgataa aaacagacat tttaaaagta atagctaatt atgcagaaat
    24961 agaagatgat ggccttgaaa taaaaatgtc taaatgcaga ggtgagcatg atgacaaacc
    25021 tgtatctgca ttagttgcta atataccact aaagaatata aaagatagat gtatgtaatt
    25081 agtgcttaaa agggggatat ccttattggt atatgcccct tttatttaat tatatgttta
    25141 aagatagaaa gacggtgatt ctttgaaaaa aatcaatatt aattataagt cgacaattaa
    25201 gttgataaaa cagttagact ggaaacttat agttacagta ttagctatct ttatttttgg
    25261 gcttgttata cttagtagtg caacacatgc taattcaaca ggttcatata atcaattgat
    25321 taaacaaggg ctagcttttg tacttggtat aggaatgatt atagtcatat tattttttga
    25381 ttataatctt ctaggcaggt attataaagc actttatata ataagtcttg ttttgttagc
    25441 catcgtttta ttacctggaa ttgggacagt aaaaggtgga gctaggtcgt ggattaactt
    25501 agggccactt gacttgcaga catctgaaat agtcaaatta acatttgtac taagttatgc
    25561 aaagatactt gaatccaaaa aggataaatt aaatacatta aaggaagtta tgcctgttgt
    25621 agtttattct cttccattta tcggattatt gattgcacag ccagacttgg gaacaggaat
    25681 tgtattttgt tgtatgatat ttgcaatgct gttcacagca ggtcttagtt caaagttgat
    25741 aaaaagagga ataatcatac tattggtgtc aatgccactg atgtatttaa tgatggcaga
    25801 ccatcaaaag ataagaatag aagcttttct aaatccagaa gatgtaacac taaaaggaaa
    25861 ctatcaagtt atgcaatctc tgattgcaat tggttctgga ggtgtaactg gtaaaggact
    25921 ttataatgga agtcaaaatc aagaagattt tttacctgta caagatagtg actttatatt
    25981 tgctgttgta ggtgaagaac ttggtgtaat aggaatggca gtgttaataa tcttatttat
    26041 gatattccta ttaaggcttt tagcaattgc aagagatgca aaagattttt atggaaccct
    26101 catcgtggtt ggtgttatgg gaatgtttgg ttaccagata atccaaaata taggtatgac
    26161 agttgcgtta ataccagtaa ctggagtaac attacctttt gtaagttatg gaggtagttc
    26221 gttattgaca tctttagcaa acttaggatt agtactaaat gtgtgtatga gaagaaagaa
    26281 aataaatttc taaatttaaa tacatctaaa cataaattga ggagattaga tatatgaata
    26341 tagcgttagt agcacatgac caaatgaaaa acactatggt cggattttgt ataggttatg
    26401 aatcaatttt aaaaaagtat ggactatatg caactggaac tacaggaaaa agaataatgg
    26461 atgaaactga attaaatata aacagattag catctggtcc tttaggtgga gaccaacaaa
    26521 taggctcttt aatagtaaca caagagatag atttagttat ttttttaaga gacccattga
    26581 catctcaggc tcatgaaaca gatatacaag ctttaataag actttgtgat gtttatcatg
    26641 ttccaatagc tacaaatcta gcttcagcag aaatatttat aaaagctctt gatagaggag
    26701 aactatcatg gagagaggtc agaaaaagta aaagtcaacg tgtttaaaaa agtaaatagt
    26761 atacaaaaat taggagatgt atcaaaataa aaatttgaat catctccttt ttatataaaa
    26821 atagaaatga tatttctaga aattaataac ctattcgata attattcaga aaaagtactg
    26881 tatatcactg ctttttctca tttgaaatat atgtatagat agtctaatac cagtagacta
    26941 tctgtttctt tatattataa atataattga gatttctaat gtaactatac tttccaaaaa
    27001 atgtattgaa tatacaaatt ataatctgta taatgttgtt aggggagaat gtatgaagtg
    27061 tatacaactt taattttaaa tgtattattg acaattctcc tcactctcgc cattactaat
    27121 aattggaaaa ttgacatatc tgttacagac caacattttc gaatatagta attattatca
    27181 ttttattgtg atgatatcaa aaatgcaact ttgcgagttg gattgtatac gaactatata
    27241 ggaaatatag aacagatttt ccaagaaatc taaatattct atgactacgt gaattgctat
    27301 gcacacatgt tgataatcca cattttaaat ctgaaatgaa cttaattaat ggtagggaat
    27361 tgaaagtaaa aatatattat aaaggagaac gctaatatga aaaaaatcag gaaattagct
    27421 actaaactat taattgcaac gatggtttta acctcatgta tgagtgtaac agtttatggt
    27481 atgacagcta aagacgttac tgcaaagaca ccgaaaagct atgtagctgg aactaacagt
    27541 atatatggtc caaagttgtc acaagctcaa cttaattcgg tagcacaagc aacagcagac
    27601 tttatgaaca agaaaattac gaagaacatg accacagatg ctaagattat ggttgcttac
    27661 aatcacatta aaaataacac aacatatgtt gactggaatg cagcagaggg agccaatact
    27721 gcctatacat tggttactaa aaaaggtgcg tgctctggaa tgtgtagaag tatgaaagct
    27781 ttatgtgatg ctatgggaat tgaatgttat tatgtacata gtatagcaaa tgaccaccag
    27841 tggaacctta ttcgttttgg tgatggacgt ttgtatcatg tagacattga tgccaacaaa
    27901 tcagcaggaa aggacattat atacaagagt cttgctcacc cattaccatt tgacaagaac
    27961 gcatatccag ctgttggagc agtaaaggtg caaaaaccag catcacctgg gaagcacact
    28021 atgctagcag atcaagaagg ggcaatactt aatgaagttt atggaaataa tatgaagcca
    28081 caaaataaaa tgtatgaggg ggttccatgt agttcaattg actcatcaat aaatacaatt
    28141 aagtttacaa gtagtaattc aaatttagac ccatcaaagc atacgatttc tgtaatagga
    28201 tttatgaaaa caccatatgg tatggttgca ttagcaccag atagtaatcc tgttagttat
    28261 ccttttgaat atgataagcc attttcttta gattttactg gtaagaaccg ttctgcagca
    28321 gaattgtcaa aatactgtaa aaaaaataat ataattatta aagtggaaat tggtgaaaca
    28381 gcatctaatt ctggaagagg tatggtagca tacgctagat acgtgaatta atacaaattt
    28441 aataaattta taaacaatgc accctcttgt tgagggtgca tttacattat atcagttatt
    28501 ttaaataata gtatgtgtga ttaatgacta tttaactaat tttgcaaatt ctgcaccaaa
    28561 tgccatacac tcttttaatg tctcttcact agggaagaat tttttcttaa gagattctac
    28621 aggcattttg aatgacatag atttaagtaa agtctcagcc atagttatac cttcaccact
    28681 ccaaccaaat gaaccaaata ctcctgctat cttgccttgg ttagccattg ggtctataac
    28741 agagaataaa tcccacatag gtttaaccat agttttgttt atagttggag aacctaataa
    28801 tattactttt gatactacta atgtatcgtg catttctgtt aatgtcatat cttctaaatc
    28861 atataattca gcctttgcac cttctttatc aagtccttct tttatctttt tagccatctc
    28921 taaagtatta ctataagctg ataagtagaa tatagaaact tgattttgat tagttgtatt
    28981 aacagcttct gtagaccact caacatatct tttaacagca gccattgggt ctttagtaag
    29041 cattggacca tgagaagtca atatagtatc aaattctatg tttagtccta ccaccttatc
    29101 tacagcactt aaaacatgtt tagcaaatgg cttaactata caatcatagt agtgtttagc
    29161 tgattttaga taatcttcac tattcactac ctctgcatca acacttgcaa agtgacaacc
    29221 aaatgcatca caagttaaaa gagtcttgtc ctcctcaatg taagtaaaca ttgtgtcaac
    29281 ccaatgtaag aatggagcag ttataaatct aagatttctt ttaccgatat ttaaaatttc
    29341 accatctttt attacatgac agttaaaagg tctatttatt tgaccatcta agtatagttt
    29401 agcagcttta gtacaataaa cttctatatt aggatttata tctaataagt attttaaact
    29461 acctgcatgg tctggttctg tgtgatgaat tacaacatag tcaattttag cgatatcagt
    29521 aacctctgat aaattactta aaaactcatc tttaaaattt gcctttacag tgtcaaataa
    29581 aaccgttttt tcgtccttga ttaaataaga gttgtaagtt gtcccaaact cagtttccat
    29641 tattatgtcg aatactttta aaccttcatc tactacgcca gtaaagtaga tatctttttt
    29701 tacttcaaaa actttactca tttttaaccc tcctaaaatt tatcattata taagtaacat
    29761 accccatttt gaggtgaatg aaacaaaaga tgtcatgata agtcatgtaa aacaatagtt
    29821 ttatgtttat aatatatcat actttgtaaa aaaattgtat ttcaataaaa atttaataga
    29881 aa
//
LOCUS       DAEMAE010000019        29868 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEMAE010000019 DAEMAE010000000
VERSION     DAEMAE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.019"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..254
                     /locus_tag="KPH93_000740"
     CDS             114..254
                     /locus_tag="KPH93_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687576.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(429..749)
                     /locus_tag="KPH93_000741"
     CDS             complement(429..749)
                     /locus_tag="KPH93_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG1687577.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(869..1606)
                     /locus_tag="KPH93_000742"
     CDS             complement(869..1606)
                     /locus_tag="KPH93_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG1687578.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1821..3644
                     /gene="acd"
                     /locus_tag="KPH93_000743"
     CDS             1821..3644
                     /gene="acd"
                     /locus_tag="KPH93_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG1687579.1"
                     /translation="MKKKAALATLAMLPLSVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVT
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKISFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNNNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAVSGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRGGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3859..8157
                     /locus_tag="KPH93_000744"
     CDS             3859..8157
                     /locus_tag="KPH93_000744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG1687580.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLESVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHPAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     ILRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8361..8822
                     /locus_tag="KPH93_000745"
     CDS             8361..8822
                     /locus_tag="KPH93_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG1687581.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIVNGKEMEFNRKDVAIVRLAIKF"
     gene            8852..10006
                     /gene="nusA"
                     /locus_tag="KPH93_000746"
     CDS             8852..10006
                     /gene="nusA"
                     /locus_tag="KPH93_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG1687582.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10013..10294
                     /locus_tag="KPH93_000747"
     CDS             10013..10294
                     /locus_tag="KPH93_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG1687583.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEIYDNLLMELEEYEK"
     gene            10284..10595
                     /locus_tag="KPH93_000748"
     CDS             10284..10595
                     /locus_tag="KPH93_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG1687584.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10617..12557
                     /gene="infB"
                     /locus_tag="KPH93_000749"
     CDS             10617..12557
                     /gene="infB"
                     /locus_tag="KPH93_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG1687585.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKIRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12600..12980
                     /gene="rbfA"
                     /locus_tag="KPH93_000750"
     CDS             12600..12980
                     /gene="rbfA"
                     /locus_tag="KPH93_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG1687586.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12961..13944
                     /locus_tag="KPH93_000751"
     CDS             12961..13944
                     /locus_tag="KPH93_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG1687587.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGIKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFNSKISSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13957..14862
                     /gene="truB"
                     /locus_tag="KPH93_000752"
     CDS             13957..14862
                     /gene="truB"
                     /locus_tag="KPH93_000752"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG1687588.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            14993..15922
                     /locus_tag="KPH93_000753"
     CDS             14993..15922
                     /locus_tag="KPH93_000753"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG1687589.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGNETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16026..16283
                     /gene="rpsO"
                     /locus_tag="KPH93_000754"
     CDS             16026..16283
                     /gene="rpsO"
                     /locus_tag="KPH93_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG1687590.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16523..17374
                     /locus_tag="KPH93_000755"
     CDS             16523..17374
                     /locus_tag="KPH93_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG1687591.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFVTEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLL
                     FAPYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDI
                     ILEMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDI
                     LAISCSN"
     gene            17499..19610
                     /gene="pnp"
                     /locus_tag="KPH93_000756"
     CDS             17499..19610
                     /gene="pnp"
                     /locus_tag="KPH93_000756"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG1687592.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKVVEF
                     IDGIVAEVGKEKMAVDLYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFENTLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19876..20619
                     /locus_tag="KPH93_000757"
     CDS             19876..20619
                     /locus_tag="KPH93_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1687593.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEENYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20777..22024
                     /locus_tag="KPH93_000758"
     CDS             20777..22024
                     /locus_tag="KPH93_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG1687594.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSVKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22059..22304
                     /locus_tag="KPH93_000759"
     CDS             22059..22304
                     /locus_tag="KPH93_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG1687595.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22314..23537
                     /gene="dapG"
                     /locus_tag="KPH93_000760"
     CDS             22314..23537
                     /gene="dapG"
                     /locus_tag="KPH93_000760"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG1687596.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCNAVNDEPSKRELDLIMSCGEIISGTILANLLDAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23650..24384
                     /locus_tag="KPH93_000761"
     CDS             23650..24384
                     /locus_tag="KPH93_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG1687597.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGIKEALKKLRELIKESEEEKNND"
     gene            24507..26921
                     /locus_tag="KPH93_000762"
     CDS             24507..26921
                     /locus_tag="KPH93_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG1687598.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGRNNEDEDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEG
                     TQSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYS
                     NYKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYE
                     IQPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVL
                     ESEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILY
                     KANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFA
                     DAQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLI
                     VATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGA
                     AKPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSE
                     AIEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDL
                     QNLEGE"
     gene            26924..27982
                     /gene="mnmH"
                     /locus_tag="KPH93_000763"
     CDS             26924..27982
                     /gene="mnmH"
                     /locus_tag="KPH93_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG1687599.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYDKIINFNDTQKA
                     LEDVIDLYESILEGENEC"
     gene            27976..29310
                     /gene="rimO"
                     /locus_tag="KPH93_000764"
     CDS             27976..29310
                     /gene="rimO"
                     /locus_tag="KPH93_000764"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG1687600.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADAIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGNYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29297..29839
                     /gene="pgsA"
                     /locus_tag="KPH93_000765"
     CDS             29297..29839
                     /gene="pgsA"
                     /locus_tag="KPH93_000765"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG1687601.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLVMLFNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENGLILNIGGVLILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        12037 a         3176 c         5709 g         8946 t
ORIGIN      
        1 tgtcaaaaaa taaaaaaatt tttatttatt agagtttttg gaaataataa taacagatat
       61 tataattaat taacagtttt tgtaagtatt tgacaataga gagggtgtaa caaatgggaa
      121 taatagagtc agcatcaaaa ttggctgaga tggttcattt attggctgtt gaaaagggga
      181 taactgatat agaagcatgg gatgaagctg tgaaagaata cagcaaaatt tatgaagaaa
      241 gaagaaatga atagaatgat aaaaagtgaa aacttgatga taaaaaataa ctttatttaa
      301 agaaattcaa tttttataga aatattaaaa ataatataaa taaaaagatg gtatttcaaa
      361 tgtaagtaaa aaatcatttt gggataccat ccttattatt tattttaata atatataagt
      421 aataaatctt acttatccat attttttatg taacctcttt tttctagctt ttttatatgc
      481 tccttgcgta tttcatcaag attaatacca tattgttctt ctagcttaaa catcatagta
      541 acacatgtct gagctacatc cattagttcc ttagcagttt tctttataat atcaacttca
      601 tctaaatcat tattttctcc acttagattt ctgaatttac ctatacattc agctaattca
      661 ccttgttctt cacccatttt gataaatgta gattctagtg ttgggtctaa attattaagt
      721 tctggtaaag atatagtttt tagtttcata aaagctcctt tcaagttatt cgtatttata
      781 ctaaaacatc ataacaaaaa taatactaaa aaaaaacttt aaattcaaca taaaaacttg
      841 catatataat aaaaatcgtg aaataaatct atatttgtct taatacagct tcatataaaa
      901 cttctccaat aggtttattt attacaagag aagctgactt atcatagacc gtatttgctt
      961 tattaataag agcaatataa tcacctttgt aataatctat aaaacttgct gctgggtaca
     1021 cagctaatga tgtacctcct ataatcaata aatcagcatt acttatagca gaaattgttt
     1081 tagtaatgac atcactgtca agagcttcct cataaagtac tacatctggt tttactatag
     1141 aaccacagtt gtcacagtaa ggaatattac ctcccagatt taacatagat tctaagtcaa
     1201 aaaacttacc acatttagta caataatttc tatgtacaga accatgaagt tctagcacat
     1261 ttttacttcc agccatttga tgaagtccat caatattttg agttatgaca gctttaagct
     1321 tacccatttc ttcgagcttt gctaaagcta tatgagcatt attgggtttt gcattaggat
     1381 atattaattt gtccttataa aattcaaaaa attcttctgg atatctaaca aaaaaagtat
     1441 gtgaaactaa ttgttcagca gtaaattgtt tattgagttt ttggctaaat aatccagtgg
     1501 agcttctaaa gtctggaata ttagattcag ttgaaacacc tgctccacca aaaaatacaa
     1561 tattattgtg attagctatt aagtctttta agctatttgc atccatagtt acacctccaa
     1621 attattatat ataaattgta ctacatatta ggtcgtttat gttttattaa tttaaataaa
     1681 tatatagttt aataagttaa ataaagggaa tataaataaa atgtatacat aatataaata
     1741 gacaatattt actaaaagaa taagttgtcg tataattata ttaaaattgt tttagtgtat
     1801 aagaagggcg ggggaagtac gtgaagaaaa aagcagcttt agcaacatta gctatgttac
     1861 ccttaagtgt ggtaaatgca catgctgatg gagatatagg tatagtgact ataaattatt
     1921 taaatgtaag aaatgaacca actgccgaaa gtagcatagc ctttgttgct aaaaaagatg
     1981 ataaagtttt gattaaagac tcttctaatg gatggtataa aataaaagct gaatctggac
     2041 aagaaggttg ggcttcatca aaatatatag caaaatcaaa tagtgactct ttaagaacgt
     2101 ctacaaataa agaaaaacaa gttacttcaa atagtttaaa tatgagaaat ggagcaggta
     2161 caagttacag agtaataact gtactaaaaa aaggtcaaaa agtagaagta atatcagaga
     2221 gtaatggatg gtctaaaatc aaatatgatg gaagattagg atatgtatct agctcttatt
     2281 taggagacgt ttcaaactca actaataaat caaaaactaa acaagtgaat acaacttcac
     2341 taaatgtaag aagtggacca aatacaagtt atggcttgtt aggcaagttg ccaaaaggaa
     2401 gtaaagtaga agtaatatca gaaagcaatg gatggtccaa aataaagtat aatggaaaag
     2461 atgcctatgt atctagtatg tacttatcgg atgtaagtca aagtaattca gacaattcta
     2521 gtcaaagcaa tgataaaaaa aatactgata aggttgtaaa tacagcttct ttaaatgtaa
     2581 gaagtggacc aggttctaca tatagtaagt tgggaaaagt ttataaagga agtaaagtaa
     2641 ctgtactatc agaaagtagt ggatgggcta agattagttt taacaataaa gaagcatttg
     2701 tagtaggcaa ttatttatct acttcagcag atacttcaaa taataactca aatagtaact
     2761 ctgataacag ttctaataat aatggtaata attcttcatc atcaggtcaa gtaaatggta
     2821 tgtcaggtat aagtggggct aaaattgatt ataaatcttt gagttatact ttggaatctc
     2881 atataagtaa acaggttgag aaagcagtgt caggaggaaa tgtgatagcg ccaagtaata
     2941 ggaaaagcac tccaagtcct gaatttagta ctttttcagc ccaaagaaca agctcatttg
     3001 taaatgcaag ttcaagtgat atagaatatt atttaaatcc taaaaacttt acaaatacta
     3061 ctaaaggtat gatgcagttt ttaaagatta acagttatag aggaggtatt tcagaatcaa
     3121 gtcttaactc gtatcttaat ggtttatctt ctagtgtatt taaaaaccag ggagcagctt
     3181 ttataaatgc tgccaagaag tataatatag atgttgtata tctagtatct catgctatgt
     3241 gggaaactgc ttatggtaag tctacacttg cccaaggtca aacattaaca tcttataagg
     3301 gacaacctct tagtaagcca gttaaagtat acaatttttt tggtatagga gcaatagata
     3361 aaagtgctaa tgtttcaggg gcagaagcag catattccaa cggttggact agtgtagaag
     3421 ctacaataga tggctctgca aagtggatat cacaaaatta cgtaaatagt tctaagtaca
     3481 atcagaatac tatctacaag atgaagtgga attatgatta tacctggcat cagtatgcaa
     3541 ctgatgtaaa ctgggctaat ggaatttctg gaattatgga aaatttaatt ggtctttatg
     3601 gaggaggaag tagtttggtt tttgaagttc ctcaatacaa gtaatacata aataaaaaat
     3661 atagactatg ggtagttttc attttattta aattatctat agtctttttg tatataattt
     3721 ataatctgta aatttaatgt taacaaaact tttatgttat aatattaatt ctaagtaaat
     3781 ataagtgagt aataggtaga aatagataaa aagtggtaat aatatatata aagataattt
     3841 tgagaaagga ggatatatat ggaaagcatt aaggaatatt tggataagtt agaaattaat
     3901 aatagtggtc ttggaaagca acttaaagaa gtttatataa atagagtagt ttattttaag
     3961 gaagataaga tagtttattt ttatctgact tctaaagata ttgtttctca tgaactttta
     4021 gataaattta aagaagaatt aatgtataaa ctcgattatt ttaaagatat gaggatgaag
     4081 ataagattta ctggtcttga aagaaaatcc aataaagatg ttataaaaaa atattggaat
     4141 aatatattgt atatattaaa gtatctttgt ccatctattg cagggtggta taaacaagta
     4201 gaatttcttt gtttagagga agaattaaaa ataaaacttc caaaaggtat attttatgaa
     4261 cgattaatga aaaaaaatgt aacttatgta cttaaaaccg tgcttagcga agagttagga
     4321 ttagatttaa atataacaat agaaaaagct gttgatgaaa aggtaaataa agagagactt
     4381 ataagaatca atgatagaga aatggaagaa aaaattaggg ctttagaaat tggaaaagtt
     4441 aataattgtg aagaaaatga agaggaaagc tatgtcatta agtctgacgt agatgaaaat
     4501 ttaatctatg gagataatgc aaatgctatg attgaaaata tagttgaatt aaatgcatct
     4561 tctggaacag ttgcagttgt tggagatata tttgatgttg acactaaaga acttaaaaat
     4621 ggaaaaatac ttatgatagc ttcaattaca gattatacta gctcaataag ctgtaaactt
     4681 tttttaacag atacgaataa agatggagta cttgaaagtg ttaaaaaagg agcatactta
     4741 aaaatcaagg gtgacatagt ttatgataca tatcaaagag aaattagtat gatgattagt
     4801 gggataagaa aagaaactag aatagagaga gaagataaat cagaggaaaa aagagtagag
     4861 ctccatgcac atacacaaat gtcttctatg gatgctattt gttctgttaa aaagcttgta
     4921 gagcgtgcag ccaaatgggg tcatcctgct attgctataa cagaccatgg tgttgtacaa
     4981 ggatttccag atgctatgaa tgcaggtaag tcaaataata taaaaatact ttatggagtc
     5041 gaagggtact tggttgaaga tgattctctc attatagagg atgcaaatga taaagaactt
     5101 tcacagacat ttgttgtatt tgatatagag acaactggtt tttctaatac aaatgataaa
     5161 ataactgaga ttggtgctgt aaaaatagaa aattttaaaa ttgtagatag atttagtgag
     5221 ttaataaatc ctgaaaaaga tatttcttat aagatacagg aattgacagg aataactaat
     5281 gaactaatta aagacaagcc aaccattgaa gaagttcttc ctaagtttat ggaatttgtt
     5341 ggagatagtg tattagtagc acataatgca gagtttgata ccggatttat atcgcaaaaa
     5401 tgtagggaac aaggcttgtt atataataac aaaaaagtag atacacttat gttagcaaga
     5461 gtaatgttgc caaacttaaa aagacataga ttaaatgtcg ttgcaaaaga acttggtatt
     5521 cctcttttaa atcaccatag agcagtggat gatgcggagg ctacagcact tatatttaat
     5581 aaatttttgc agatgcttac agaaaaaggt gcaaaaactt taagtgatgt aaataatata
     5641 cttggaaaga ttgattacac aaagcttagc acaaatcaca taaccttgat agcaaaaaat
     5701 tctataggta taaaaaattt atataaaata atttcagatg cacatgtaaa tcattttttt
     5761 agagcaccaa gaatccttag aagtgtatta aatgaatata aagaaggatt aataattggt
     5821 tcggcttgtg aagctggggt ggtttttcaa gctgtaaaga aaaacgtgtc tgatgaggaa
     5881 atgaaaaaaa taatagattt atacgattat attgaagtaa tgcctataga taacaataga
     5941 tttatgattg ataaaggaga agtaaaggac gaagaagagt taagggagtt gaataggaaa
     6001 ctaattgata cagccaaaaa atttgataaa attccagtag ctactgggga tgttcatttt
     6061 ttagataaac atgaagctat cttaagaaaa gttcttaaat attctcaagg ttttaaagta
     6121 gatgaagaag aaacttattt acattttaga acaacagatg aaatgttgga agagtttagt
     6181 tatcttggag aagaattggc atatgaagtt gtagttgaaa atagtaatct aattgccgat
     6241 atggttgaag atataaaacc tataccagat gatacttatc ctccaattat agagggttca
     6301 gatgtggagt taagagaaat gtgttacaaa aaagccaaac gaatctatgg agacccaata
     6361 ccagaaatag ttcaaaaaag actggataga gaattaaatt caataataag caatgggtat
     6421 gcagttatgt atattatagc tcaaaaactg gttgcaaaat ctctttcaga tggctattta
     6481 gttggttcaa gaggctcagt tggctcatct tttgcagcta ctatgagtga tataacagaa
     6541 gttaatcctc ttccagcaca ttatatttgt gaaaacgaag actgtaaata ttcattcttt
     6601 tatgaaatag gtgaatgggg ttcaggtgtt gatttaccag acaaggattg tccaaagtgt
     6661 ggaagaaaac ttgctaaaaa cggtcatgat attccatttg aggttttctt aggttttgaa
     6721 ggtgataagg aaccagacat agacttaaat ttctcaggaa attatcagcc aacaattcat
     6781 aaatacacag aagaactatt tggagagggg tatgtatata gggccgggac tataggtact
     6841 gtagcagaaa aaacagcttt tggatacgca agaaaatatg tggaagaaaa taatataagt
     6901 gtaccaaatg cagaagtact taggctttca aatggatgta ctggagtaaa aagaacctca
     6961 ggacagcatc ctggtggagt tatggttata ccagactata aagatgtata cgattttaca
     7021 cctatacagt atccagcaaa tgatacttct tgtggtgtta taactactca ctttgattat
     7081 cattcaataa gtggtagaat acttaagctt gatatacttg ggcacgacgg tcctaccatt
     7141 ataaggatgc ttgaagatat gactggaata aatataacag aaattccgtt agatgataaa
     7201 gagactatgt ctctatttac atctacagaa gcattaggtg ttacaccaga agaaataaac
     7261 tgtcctatag gttgccttgc tgtaccagag tttggaacta agtttgtacg acaaatgtta
     7321 cttgacacaa aacctactac atttgcggaa cttgtacgta tatctgggct ttctcatggg
     7381 acagatgttt gggtaaataa tgcacaagac ttagttagag aagatatagt tggattaaaa
     7441 gatgttatat ctacacgaga tgacataatg aactatttaa tatttaaagg attacctccc
     7501 aaaatgagtt ttacaataat ggagagtgta agaaaaggta aaggtctaaa accagaacac
     7561 attgaagaaa tgaaaaaaaa taatgttcca gaatggtata taggctcatg taaaaagatt
     7621 aagtacatgt tcccaaaagc acatgctgtt gcttatgtca tgacatcatt tagaattgca
     7681 tattgtaaag ttcactatcc agaagcattt tatgcgactt attttacgac aaaggttgaa
     7741 gattttgatg cagatttaat tgttaaagga ttagatgcta taaaatcaaa aataaatgaa
     7801 attgagtcac tgggaaatga tgcaaccaca aaagaaaaag gtatgttgac cgtattagaa
     7861 gttgctcttg aaatgtatgc tagaggaata aaattattac ccgttgatat atataaatca
     7921 gatgcgactg agtttatagt tgtaggagaa aaaacattac ttccaccaat ggcagcaata
     7981 caaggtcttg gaggaaatgc ggctataaat atccaaaatg aaagaaaaaa tggtgaattt
     8041 atatcaaaag aagatttgag aaagagaaca aaaatatcaa aaacggtaat agaaactctt
     8101 actaatcatg gttcacttga gaatatgagt gagagaaatc aattgtcatt gttttaagtt
     8161 agcccaaaaa gttgcaaata aaagaatttt atgatatcat agtatagaaa atagtatact
     8221 ttgcagaaac taagagtgag acagctgttc tcactctttt attttaaata tattattaaa
     8281 aagctggttt aatcttatta aatatatttt atttaagatt atataaactg gtcacttaca
     8341 attgaatagg aggaacttta atgaaaaaaa atttagaagc tacaattgag gagattgtaa
     8401 ctaaaataac agatgaacat ggatttgaaa tggttgatgt tgaatatgta aaagaagcag
     8461 gagaatacta cttaagagta tacatagata aagaagaagg aatatcttta aatgaatgtg
     8521 aattagttag tagagaatta agtccaatac tagatgaaaa agaccctata aaagagaatt
     8581 actttttaga ggtatcttct ccaggacttg atagagcatt aaaaaaagac agagattttg
     8641 ttagatatca aggtagagat gtagatttaa aattatataa accattaaat ggatgtaagc
     8701 aatttgaagg tgaacttgta ggattaacag aagataataa tataaaaatc attgtaaatg
     8761 gtaaggaaat ggaatttaat agaaaagatg ttgctattgt cagattagca ataaagtttt
     8821 agtggtatat taaataggga ggatataaaa aatgaatcat gaatttatgg aagcattaga
     8881 tgaattagta gttgacagag gtatagataa agaaatactt atagatacta tagaacaagc
     8941 tcttttaact gcatacaaga aaaactttgg ttcagcacaa aatgttagag ttgaatttga
     9001 tagagacaag ggagatataa aagtattttc tcaaagagtt gttgttgatg aatcagattt
     9061 atatgatact ttcttagaaa tagagttagc agatgctaga gagataaatc ctaattatga
     9121 attaggtgat attatagaac atgaagttac acctaaaaac tttggaagaa ttgcagctca
     9181 aacagcaaag caagtagttg ttcaaagaat aagggaagct gagagagaaa tagtgtataa
     9241 tgaatttata gaaaaagaaa atgaaatagt tacaggagag gttgctagag caaataaaaa
     9301 tgttgtttat gtaaatcttg gaagaataga aggtataatg actcaaagtg aacaaatacc
     9361 atttgagaat tataaagcag gtcaaaaaat aaaattctat atacttgaag ttaaaaagac
     9421 taacaaagga cctcaaatag ttgtatcaag aagtcatcca ggtctagtaa agagattgtt
     9481 tgaagaagaa gttcctgaaa tatatgaagg aatagttcaa ataaaatctg tagcaagaga
     9541 agcagggtca agaactaaaa tggcagtaag atctatagat gaaaaaatag acccaattgg
     9601 agcttgtgtt ggaccaaaag gacttagagt aaaaaatata gtggatgaac ttggcgatga
     9661 gaaaatagat ataataaaat atagtgaaga cccagcagag tttatatcag cagcacttag
     9721 cccatcaaaa gttgttagag tagatataga tgaagatgaa aagtctgcct tagtaatcgt
     9781 tccagattat cagttatcgc ttgctatagg taaagaaggt caaaatgcaa gactagcagc
     9841 aaaattgact aactggaaaa tagatattaa gagcgaatct caagcagaac aagaaatgtt
     9901 aaatcttgaa aattccaaag aaatagaagc tgaggatgtt gaagctgaat ttatcgacca
     9961 agaagagtta gatacttcag aggataattt tagtgaagaa gagtaaggag ggatgatttt
    10021 gaaaaaagta aaaaaagtgc ctcaaagaaa atgcatagca tgtcaagata gagactctaa
    10081 aaaagagctt ataagaatag ttaaaaataa agaaggaaaa atatttttag acccaacagg
    10141 aagagccaat ggaagaggag catatgtatg taaaagctct gaatgtctaa aaaaagctat
    10201 aaagagtaaa gcattaaata aagcttttaa aatagatgtt cctgatgaaa tctatgataa
    10261 tctacttatg gagttagaag aatatgaaaa ataataaaga aaaagtactt tcgttcttag
    10321 ggctcgcaac tcgtgcaggg aaaatagtgt ctggtgatga ttcaactttg cttgacctta
    10381 aaaaaggaaa agtaaatttg attttaatag cagaagatgc atcaaataat actaaaaaac
    10441 tttttaaaga taaatcaact tttagaaata ttccatatct atttttttca acaaaagagg
    10501 aaataggatt tgcaatagga aaatctccaa gagcagttgt aggaataaaa gatgaaaatt
    10561 tttctaaaaa gataattgaa ctaatagaaa tttaaaataa aatagggggt gatcttgtgt
    10621 caaaaacaag agtataccaa atagcagaag aattaaatat atcaaatgaa gagcttataa
    10681 ataagttagc tgaattagat ataaatgtaa ctgacaaaga ttcagtatta gaaggtgaag
    10741 agttagaatt agcacttgaa atgttaggtg aagatttatc tcaagaaaat ggaaatgtta
    10801 tagaaataga tggaaagttg acagtacaag tattagcaac gaaattagat aaatctccat
    10861 cagaaataat tatgaagctt atgaaaatgg gaactatggc aacaataaat caagagataa
    10921 gttttgagat tgctgctttg gctgccaaag actatggatt tgaattaaca gtagctgaaa
    10981 gtgatgatac agaagcttta gaaatagaag ctttaatgga aattgaagaa gataaagaag
    11041 aggatttaaa acctagacca ccagttgtta cagttatggg acatgttgac catggtaaga
    11101 catctttatt agatgcaata agaaaaactg atgtgatttc aggtgaagct ggaggaataa
    11161 ctcagcatat aggtgcttca gaagttaaaa tcaatggaca caaaatagta tttttagata
    11221 ctccaggaca tgaggctttc acatcaatga gggcaagagg agcacaagtt acagatatag
    11281 caatattagt agttgcagca gatgatggta taatgcctca aacagttgaa gctataaacc
    11341 atgctaaagc agcaggtgta cctttaatag ttgctattaa taaaatagat aaaccaggtg
    11401 caaacccaga taaagttaaa caggaattag cagaccaagg attattagtt gaagactggg
    11461 gtggagaagt aatagcagtt ccagtatcag ctaagaagaa agaaggaata gatacacttt
    11521 tagaaatggt attattggtt gctgaaatgg aagaactaag agctaatcca aataaaagag
    11581 cagtgggaac tgttatagaa gctgagcttg ataaaggaag agggccagtt gcaacagtac
    11641 ttgttcaagg tggtacttta acagtaggtg acccaatcgt tgcaggtgtt gcatgtggta
    11701 aagttagagc aatgataaat gcaaaaggta agagagtaaa aacagcaggt ccatctacag
    11761 cagttgagat attaggtcta tcagaagttc cacaaggagg agaccaattt gtcgaagttc
    11821 caactgataa gattgctaga agtgtagcag caagaagaca acaaatagtt agagatgaaa
    11881 tgttaaaatc aactcaaaga ttatcactag atgctctatt tagtcaaatg agtgaaggtt
    11941 ctataaaaga ccttaatata gttataaaag cagatgtaca aggttcagta caggcagtta
    12001 aacaatcttt agaaaaatta tcaaatgaag aagttcaagt taaagtaata catggtggtg
    12061 ttggagctgt aacagaatca gacatattac ttgcagcagc ttctaacgca ataataatag
    12121 gattcaatgt aagaccagtt ccaggtgcag aatctctagg agaaaaagaa aatgttgata
    12181 taagaacata tactataata tacaaggcaa tagaagatat acaagctgct atgactggta
    12241 tgttagaccc tgaatatgtt gatgaagaaa caggaaaagc tgaaataaga gaaatatata
    12301 aaatatctgg tgttggaaca gtagctggat gttatgtgac taatggtaag atatttagaa
    12361 attgtaaaat aagattagtt agagatagca taataataca tgaaggtgag cttgcagcac
    12421 ttaagagatt taaagatgat gtaaaagaag ttaattctgg atacgaatgt ggtatgagtt
    12481 ttgtaaatta taacgacata aaagaaggag atatagtaga agcttatata actaaagaag
    12541 tagaaagaaa attataaatt cgtatcaaag aaaatcagtt agtaaagaag gtcgatggta
    12601 tggcatcata taatagaaca agaaggatag cagaagaaat aagaaaagtc gtaagtacta
    12661 tgcttataaa tggagtaaaa gaccctagaa taacatctat ggtgagtgta actgatgtag
    12721 aagttacaaa tgacctaaga tatgcttatg tatatgtaag tattctaggt ggagatgaag
    12781 aatccacact aacaggtctt aaaagtgctg gaggatatat aagaagagaa gtaggaaaaa
    12841 atataaaact tagatatatt cctgagatag tatttaaact tgatgattca atagaaaaag
    12901 gaatgtatat ggatagcctt ataaaaagag ttaatgaaaa aaatgctcaa caaaatgagg
    12961 atgaaaatta tgatgaataa tatggatagt attataaact atatttatga gggtgactat
    13021 tttatagtta cctctcatat cagtccagat ggagacaata ttggttctac actttcaatg
    13081 tattatacat tgaagaattt aggaaaaaat gtatattatg tacttgatga tgaagctcca
    13141 ttaaatttaa ggtttttggt tgaaggtata aaaatctata agtctaatga atttaatatg
    13201 aagaactatt ctctaattgc acttgattgt ggggataaga tgagaatatg tgtgagtgat
    13261 gaaatcaaaa ataatgcatc aaaattaatc tgtattgacc atcatgctag taatgattca
    13321 tatggagatt taaattatgt agatattgat gcatcttcta catgtgaact agtttataat
    13381 ttattagtta gatttcagca aacaaaagat attaatatta taaatgaaga tatagcaaca
    13441 tgtttatata caggtcttgt aactgatact ggaaactttt cgtattcaaa tgtacatgca
    13501 agtagttttg aaatggcaaa aaatttactt gttttaggtg cacaaaaaaa tactataata
    13561 caaaatatat atcaaagcaa ttcttctgat tattataaat tattaggaga agctctaaaa
    13621 ggtttggaga tatttaattc aaagatatct agtatagttt taactcaaga catgatgaat
    13681 agaaataata ttagctttaa tgatgttgat ggaataacaa catatacaag agatataaaa
    13741 ggtattgaag ttggaatttt atttaaagag aaaaaacaaa atgaaattaa agtgagtttt
    13801 aggtcaaaaa attatgttga tgtaagtgaa atagccaagt tatttggcgg tggtggtcat
    13861 gtaagggctg ctggatgtac tattagagat agtattgata atgcaaagaa aatggtacta
    13921 gaggcagtgt taaaatcaat ttaaggtgac agtaaaatga ataagattat aagtatattg
    13981 aaaccaacag gtatgacatc tcatgatgtt gtaagtagag tgagaaagat tttaaatata
    14041 aaaaaagttg gtcatacagg tactcttgac ccagatgctt caggtgtttt accaatctgt
    14101 atagggaaag ccacaaaagt atgtgaagtt attttaaaca aagataaatc gtatatatgt
    14161 gagcttactt taggtataag tacagatact tatgatgcct caggtgaaat tttaaaaaaa
    14221 gttgatgatt ttaaatttag taatgaagac atagaaagag cttttgatac acaaagagga
    14281 gagataaatc aacttcctcc aatttattca gctttaaagg taaatggaaa gagaatgtgt
    14341 gatttagtaa gaagtggtag acaatctgaa attactttaa aaacaagaag agtgaatata
    14401 aaagacataa aaatactaag tataaaaggt aataaagtaa tgttttatgt tgagtgttct
    14461 aaagggactt atgtgagaag tatttgccat gatataggag aatatctagg ttgtggagct
    14521 catatgtcgt ttttgaatag aacatcttct ggtaaatttg acttagataa ttcaataaca
    14581 ttagaagaat tagaattatt ttatgaaaat aaaactttag acaaatactt gtatgatata
    14641 gattatgtac ttgattcgtt taattatgtt gtattaaacc ctaatgctat aaaatattat
    14701 agtaatggtg gctcaataga tgataaaagg tttttaaaaa ataatttcga taaagatgat
    14761 gaatttgtaa gagtatacag tacagataat ttcttaggat taggaaagtt gtcaaaacac
    14821 aataatacaa taagtgttaa aagtgataag atgttcatat aaccaatgta tttaaaaaaa
    14881 caacaaaaga gatagtacta taataacttc tatagtctat ctcttttact tatgctataa
    14941 tagtttgtga ataagttaag agtaaattat attttagcgg ggaacattta ttatggtgat
    15001 tataaaatct ataaaagaaa tagcaaacat aaaagaaagt gtcattacca taggaaactt
    15061 tgatggtgta cacaaaggac atcaagttct tataggaaaa acagttgaac atgcaaaaaa
    15121 agaaaatata cagagtattg tatttacatt tgcaaatcat cctgtaaatt actttagacc
    15181 aaactcaaca aaaaatatta taagcaatga tgataaaata aaattattag aagatttggg
    15241 aatagatata gttgtaatca taccatttga tgagtatatg actaaaatac ctgcaaagga
    15301 tttcgtagat gagatactag taaaaaaatt aaaagctaaa aagatagtaa ttggacatga
    15361 ctttacattt gcaagggcaa aagaaggcaa tgttaatcta ttaaaaagct tagaacatga
    15421 atttggtttt gaggtagaag taataaagcc catcaaaatc aatgatgtaa gagtgagtag
    15481 tacttatatt agaagtttag tatcccaggg tgatatggct aatgtcaagg aatatttggg
    15541 tagaaattac aagttagaag gttgtgttat acattctaaa caccttggaa gaactatagg
    15601 attcccaaca gcaaatattg accttaagaa taatatgctc gttccaaaga gaggtattta
    15661 tgcttctatt gtacatattg gaaatgagac ttattttggt gctacaaatg taggttataa
    15721 cccaacagtt aatggaaaaa ctttgtctat tgaaacaaat attttagagt ttgatagaga
    15781 tatttatgga gaaaatataa ttgttgaatt tttggaaaga ataagagatg aaagaaagtt
    15841 taattcaata gatgacttaa aaaatcaatt gtataatgat acaaattatg tgtatgaaaa
    15901 ctatgtttgc aaaaacaagt aatgtatgat aaaatgatta aggaaaataa ccattactcg
    15961 gcatttgaat actccaatca atgcttagta ataggcgttt ctaaaaaaat acggaggtaa
    16021 tctaaatgat aaacaaatca gaaataataa aagaatatgg aagagctgaa ggagatactg
    16081 gttctccaga agtacaaata gctttattaa cagctagaat aaacgagtta aatggacatt
    16141 taaaaactca taagaaagac caccattcaa gaagaggact attaaaaatg gttggtagaa
    16201 gaagaaactt attagcttac ttaaaggaaa aagatcttga aggatataga gcattaatag
    16261 ctaaattagg attaagaaag taatttcatt ctttataaaa aagcaggtat agtgtatacc
    16321 tgctttttta tagttaattc attataaagt aaaataatta ataataaaag tagtttatat
    16381 gttataaaaa attataaaat gaataatatt gggtaagaat attaaaatag tatacaaata
    16441 cataatcaat ttaaaaggaa agacaggata aatatagaaa ttttataaca ggataattag
    16501 ttggaataag gaggatatta ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat
    16561 tccagatgaa atatcagaaa aagattatat agagatagtt ccacttacag ttatttttaa
    16621 tgatagggaa tatatagaag gagtagatat aaagaaagaa gagttttata aaaaagtaaa
    16681 agaaattaag caaataccta aaacttcaca agctacatat atggagttta aagaagtgtt
    16741 tgataagttc gtaacagaag gttatcatat aatctgtatg acaggggctg caaacgcatc
    16801 aggaactttt caaagtgcta tgatagctaa aaatgatgta aatgaaaatg aaaaagaaaa
    16861 aatacatata tttgatacta gaaatttatc tctaggtagt ggacaatatg ttataaaggc
    16921 atgtgaactt cttgaagaag gactgggatt tgaagaaatt atagatgaat tagaaaatac
    16981 tcgtagtagt gtaagattac tttttgcacc atatacactt gattttttaa agcagagtgg
    17041 aagagttcca gttgcaactg ctttgatagg aaatatgtta aatataaagc ctatattttt
    17101 ctttgataat ggggaagcta agttggtaaa taaggttaga ggaataaaga atatagcaag
    17161 taaattggtc gatataattt tagaaatgaa tgaagggtct ttagaaggaa aaatcgtcac
    17221 aataggctgt ggagataatt tacatgattg tgaaatatta aaagatgaag taaataaaag
    17281 aataaaagca agaagagtat tatttactcg aggaggagtt tctatttgct ctcatacagg
    17341 gccagatata ttggctataa gttgttctaa ttagtagtaa tataaggttt ttgcatatat
    17401 gtgtgaagat aattttatat tgttaattta tagtattttt gataatatag taaatagagt
    17461 gatatacgtg ttttttaata agaggaggac aagcattaat gtttgaacat aaaatattta
    17521 aaatggattt tgcaggaaga gaactttctg tagaaatagg aaagatttgt gaaatggcca
    17581 gtggtagttg tatagttcga tacagtgaca gtatggtaat ggtaaatacg actaaatcag
    17641 ccaaacctag agacggaata gacttttttc ctttaagtgt ggattatgaa gaaaaattat
    17701 actctgtagg taagatacct ggaggttttt taaagagaga aggtaagcct tcagaaaaag
    17761 ctatacttac atcaagattg atagatagac caataagacc tttattccca aaaggattta
    17821 gaaatgatgt tcaggtagtt gcaacagttc tatcagtaga ccaagattgc acaccagata
    17881 tagtagcaat gataggttct tcaatagccc tttctatatc agacatacca tttaatggac
    17941 caacaggttc agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat ccaaacgcag
    18001 agcaaagaga aaaaagttca atgcatctag ttgtttcagg tacaaaggaa gcaataatga
    18061 tggttgaagc cggagctgat gaagttccag atgaggtaat gcttgatgct attttatttg
    18121 ctcatcaaga aataaaaaaa gtagttgaat ttatagatgg tatagttgca gaggttggaa
    18181 aagaaaaaat ggctgttgat ttataccatg caggagaaga aataactcaa ttggttcgtg
    18241 aatttgcaac tgataagatg aaaaaagcag ttcagacttt tgaaaagtta gagagaatgg
    18301 aaaatatgga tagagttaaa gaagaaactt tagctcactt tgaaaatact ttagaagatt
    18361 ttgaagattt tgttggagat atagaagaag tattacaaga tataataaaa gaagaagtaa
    18421 gaaaacttat agtacatgaa aatgtaagac cagataatag aaaacttgaa gaaataagac
    18481 ctatatggtg tgaaactggc atgataccta gagctcatgg ttcagccata tttactagag
    18541 gacaaactca agtacttaat gttgctactt taggtgcttt aggagatgtt caaaaattag
    18601 atgggttaga tgaagaagaa aacaagagat atatgcatca ttataatttc cctgcatata
    18661 gtgttggaga agctagacca tcaagaggac caggaagaag agaaatagga catggtgctc
    18721 tagcagaaag agctttatta ccagtaatac catctcaaga agaatttcct tatgctatta
    18781 gattagtttc tgaagtatta agttcaaatg gttctacatc tcaagcaagt gtatgtggct
    18841 caacattatc tttgcttgat gcaggtgtac ctataaaaga tatggttgca ggtatagcaa
    18901 tgggacttat aaagcatgat ggtaaggttg cagtgctttc agatatacaa ggtatggaag
    18961 accatttagg tgatatggac tttaaagtag ctggaacaga gtatggtata acagcaatac
    19021 aaatggatat aaaaattgat ggtatagata aagaaattct acaacgtgct ttaaaacaag
    19081 ctaaagaagg tagaatccat atactaggag agatgagaaa aactatatct cagccaaaac
    19141 cagaactttc accatatgca cctaagattg ttaaaatgca aataaatcct gacaagataa
    19201 aagatgtaat aggacctggt ggaaagataa taactaagat aatagacgaa actggagtta
    19261 agatagatat tgaacaaact ggggaagtat ttatatcagg tattgagatt gatatgatta
    19321 agaaagctca agaacttata aataatatag tagttgaacc agaagtagga aaaacttata
    19381 aaggtaaagt aagtagaata atgaactttg gcgcatttgt tgagatactt ccaggaaaag
    19441 aaggattact tcatatatct catatagctc atgaaagagt tgccaaagtt gaagatgtat
    19501 tgaatatagg ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa ggaagagtta
    19561 atttatcaag aaaagttctt ttaccaaaac cagaacataa aaataaataa cttattatat
    19621 aattaaaagc aaagtcttag gattttgctt ttttaaatac aaaaaagtag ataaatttaa
    19681 actaatagtt atagatggct tatagtcctt tttctgtaag gttttactct tataaagaag
    19741 ctatttaaaa gttgattaat agaaagtttt attgatatat atagaatcac ttaagttata
    19801 tttagtgcat aaacagagct tatttttaat agtattaaat aagaaacaat ttgtacataa
    19861 ttagggggag ttttaatgat aatgatggta cttaataaga gtaagctcaa aaaaatcata
    19921 atactaattt tagtagtgtt attagtatta tcaggagggg taatggtatt aaagttattt
    19981 aacaacaagc cagtatttaa tttatttaat ggtgtagatt taccatatga gagaggtaca
    20041 aaggataaaa gtggatatgt tgcattaacc tgtaatatag accttggatg ggaaacagag
    20101 tacgtagaat ccattttaga aacacttaaa aaagaaaatg taaagattac atttaatgta
    20161 actggaaaat gggcagaaaa gaataaggat gaattattaa agataaaaaa acaaggacat
    20221 gaaatcggta atcatggata caaacattta gattattcta cattatccta tgaagaaaat
    20281 tatgagcaaa tagagacctc taaaaagata attgaagaaa ttataggtga aaaaactaaa
    20341 ttttttcaag caccagcagg ttcttttggt ccagaaacag taaaggctgc taaagcatta
    20401 gggtatactt ctatcaaatg ggatgccgat actatagatt ggaaatacaa agaccaacca
    20461 gaggtaatta ttgatagaat gaagaaaaaa gacattaaag atagcagtat aatcttaatg
    20521 catccaacca acgcaacgac aaaatgtata gatgatatta tagctattgt aagagaaaaa
    20581 ggactaaaac caggaaaact tagtgatgta tttaagtaga caaagtataa atatttggtt
    20641 aagattgtat aagttgtagt tgaattaatt aaaagactga cttataatgt aatacatata
    20701 aaaaataaat ataattaaaa catagtgttt tcaataatcc aatatgaaac aaagaataaa
    20761 ttgttaggag aatgtaatgt ataagacaaa aatactggaa aatgggctta caataattgg
    20821 tgaagaaata ccatatctaa agtcaataac attaggtatt tggataaatg caggctctag
    20881 gatagaagaa gcacaggtga gtggaacttc tcattttata gagcatatga tgtttaaagg
    20941 aaccaaaaat agaacatcaa aagagattgc tagttcaata gataatttgg gtggtcaaat
    21001 aaatgcattt acaagtaaag aatgtacatg ttattatgta aaactaatag atgagcatat
    21061 agatacagga attgatgtat tgagtgatat gatactaaat tctaaatttg ataaaaatga
    21121 tatagataag gaaagactta taatattgga agaacttaag atgtatgaag actctccaga
    21181 tgacttatct tatgatttat tagtggaaaa tatatatgct aatgatggat taggaatgaa
    21241 tataattggg acaaaagaat ctctatacaa tataactaga gaatccatgt tagaatactt
    21301 aaataagtat tatattccaa ataacgctgt tatttctata gctggaaact ttaactttga
    21361 tgatatggta gagaaaatca aatcaaaatt tggacactgg gaaaagaaaa atttgagtat
    21421 agacataagt gaggctaagt ttaatccttg ctttatatca aaaaataaag atactgaaca
    21481 ggtgaactta gctatgtgcc taaaaggtat tccttttgaa aatgatgaag aagtctactc
    21541 aatggcagtg gtaaataata ttttcggtgg aagtataagc tcaaggcttt ttcagaaaat
    21601 aagggaagaa aaaggtttag tatattcaat atactcatca cagacattat ataggaaatg
    21661 tggtgaatta ggtatatttg caagtatgag tacagaaaat ttacaagatg tatataattt
    21721 aataaagaaa gaaattgaaa atataagaga aaactatctt acagaaaaag aaatatctga
    21781 aagtaaggaa cagttaaaag gaaattatat attggattta gaaagtacta gcagtagaat
    21841 gatgtctact ggtaaatcta tgttacttag caaaaaagta aaaactacag atgaaatact
    21901 tgaatgtata aataatgtta atattaattc agtaaagaag gttgttgata aggtattcaa
    21961 tatcgaaaat attggtacat gtatagttgg aagagatgta gaaaagattc tacacttgga
    22021 ttaacattga aaaaagtaaa tctctggagg tataaaatat gaatttatct gaaatagcag
    22081 gtaaggaaat agttaacttg gtcacaggag aaagattggg ggtcattgga gaatgtgacc
    22141 ttatactaga tgaaactaca ggcggcatat tagcgctttt aattccaaaa gaaaaaggat
    22201 tttttgccag aagaaaagat aagtcatttt tagaagtacc atggagaaat gtaaaaaaaa
    22261 taggtaatga catgataatt atagaatatg agggatatat ttaggaggaa aaaatgagta
    22321 ttttagtgca aaagtttggt gggacatcag tagaatcata tgaaaaaatg aatgaagtat
    22381 gtaagatagt aaaagcatac aaaaaaaatg atgaagaatt acaattagta ttagtagttt
    22441 cagcaatggg aagaaaagga gctccatatg ctacagatac acttataaat ttgtgtaacg
    22501 cagtaaatga tgagccaagt aaaagagaac ttgatttaat aatgtcttgt ggggaaataa
    22561 tatcaggaac aattcttgct aatttattgg atgcacaggg gatagattct gttttcttta
    22621 ctggtcagca agcaggtata attacaagtg atgaatattc taatgcaaag ataaagtata
    22681 taaatcctaa aaaggtaaaa agagctttag atgatggtaa ggttgtagtt atagctggat
    22741 tccagggggt aacagatgat ggagaaataa ctactcttgg aagaggagga agcgatacat
    22801 cagcagttgc tattggtaaa gctttagaat gtgaaacagt tgaaatttac actgatgtgg
    22861 atggtataat gactgcagac cctagagtag aaccaaacgc taaagtatta agttttatag
    22921 attatgagga agtattccaa atggctgaca aaggtgcaaa agtaatacat ccaagagcag
    22981 tacagttggc taaaagtggc aatataactt tagccataaa gaatacaatg aatccaactt
    23041 ttgaaggtac aaaaataggt tctttatgta gacacttaga agataatata gaatatgaac
    23101 aagaaagaga ttttaaggtt gctgttgcaa ataaagatag tgtagcgcaa gttaagatta
    23161 agtcagcaga agaagtgttt acagaagtat taaatgagat agaaaagaaa cttataacta
    23221 tagatatgat aaatttcttc atatcagaaa aagcatttgt tgtagaagat gctgatataa
    23281 aatcattaaa ggaaatacta gataaatttg aattggatta tgaagtaaat agagactgtg
    23341 ctaaggtaac attgatatgt tcaagaattg ctgatgaaat gtctggtatc atgtcaaagg
    23401 tagttagagg attatcaaaa gcaggggtat ctcttttaca aacatcagac tcaaatatga
    23461 ctatatcttg tttagttagt gaagaagata tgcatacagc agtacatgct atacaccaac
    23521 aattttattt aaaataaact gtataataca attatttttt aatcaaattt gagaatttca
    23581 tatatatctt aatatatttg atttatttgg taaaactaaa ttaaatataa ttttgatagg
    23641 agttttttta tgaaagatta tagagaagat aatgaaagat ttgataaaga taattgtagt
    23701 gaagaatcta gttgcgaaga gagagaatgt ataaagcaat ttggtacaaa tgaaatgcca
    23761 cctcaacctc ctaaagatat tcaatgtata actataattg gagaaattga gggacatttt
    23821 ataggaaatc ctcaaaaaaa agctacaaag tatgagcata taattccaat gctatactca
    23881 attgaggaaa gtaatgatgt taaaggagta ttagttgttt taaatactgt tggaggagat
    23941 attgaagcag gtttagctat tgcagaactt ttaaacagta cttctaaaaa agtagttact
    24001 cttgttctag gaggaagtca tagtataggt gttccccttg ctacagcggg agattactca
    24061 tttatagcac caacagcaac catgattata cacccagtgc gaactactgg tcttgtgatt
    24121 ggtataaatg agacttttga atattttaaa aagatgcaag atagaataat tcaatttata
    24181 ataagaacat caaatatcaa aaaagatgta cttgaaaaac ttatgcatga aaaagatgaa
    24241 cttgtaagtg atgttggtag tgtattaata ggaaaagaag ctgttgatta tggtcttata
    24301 gatgaagttg gaggaataaa agaagcactt aaaaaattaa gagagcttat aaaagaatct
    24361 gaggaagaaa aaaataatga ttaaaatttg tttaaataat aaaatttatg atataatgat
    24421 gtaataaagt aggagtgtta gtgttaaggc attagcactt tttgctgtga aggggtaaaa
    24481 cttgctaaaa ataaggagtg atattaatgg ccacgaaaaa gaagaaaaaa aagaaggtaa
    24541 ataccttaag ttttaatgca gaatatcata atttaataac aatatttata ggtgtatttt
    24601 tattatatag tttaaattct aattctatgg gatggatacc tgtgttaatg caaaatcttt
    24661 ttaagggact atttggaggg ctttcaatag caataccatt tatagtaatt ataacaggac
    24721 ttttaggatt ttttgatgga aatgaatata tatataggct aagaaagact aaactatact
    24781 acataattat tttatttata tttgtatttt atggattatt aaatgcagga actcttcctg
    24841 tagatagccc actaaaaggt aacatgtttg atgatgttat gaaattgggc gtaagtggac
    24901 aaggtagtgg tttgatagca acaactattg cgtattatat gagtaaaata tttggtatag
    24961 caggcggatg gttaataagt atcttcgcgc ttatactttc tgtaatgttt atatttaata
    25021 tttctataaa agatttggtg tcaaatgcaa aatcaaaatc gaaagcatca aaagacagca
    25081 atttaacgtt taaagataaa attgcaaata tgaaaaaatc tgcaatagat atgatgactg
    25141 acgaagtaga tgatactact ataaataaaa agccaggttt ctttaaaggg ttaatgtcaa
    25201 aaggtcgtaa taatgaagat gaagatgaag atgaatattt tgaagaagaa aatagtgatg
    25261 gtgtagatga taaaactata aaaatagttg gatttaataa ggcagaagat gagtatttag
    25321 aaatattaga aggaactcaa agtatgccag agttagatgt tttaaaagaa ttacaaaaag
    25381 ctactaatga aaatccagtt gtagatacta aaccagaaaa aaaagttgat atagctaaac
    25441 ctaatttaaa tatagaaaaa actcaaccta tgagtattgt agcagagcca gttaatgagg
    25501 attattcaaa ctataaaaag cctagcattg agcttttaaa taaagtaaat aagaagtctg
    25561 atgaaaatgg taaaaagaaa gtgctgaaaa atgcttctct tttagaaaag acactatcag
    25621 attttggagt ggaagcaaaa attaatcaag ttacagttgg acctactata acaagatatg
    25681 agatacaacc aagtccagga gtcaaggtaa gtaaaatcgt aaatttaact gatgatatag
    25741 ctttaagttt agcagctaaa agtataagaa ttgaagcacc aattcctggt aaaagtgcaa
    25801 ttggaataga agttccaaat gaagaagctc aaatggttgg tgtgagagaa gttctagaaa
    25861 gtgaagaatt taataatttt aactctcctc ttgcaatggg actgggaaaa gacgtagcag
    25921 gaaaaattat aattggagat ataggtaaga tgccacactt actaatagct ggttctacag
    25981 gttcaggaaa gagtgtgtgt gtaaatacat taataagtag tattttatat aaggcaaatc
    26041 ctgatgaagt aaaattacta ttaatagacc caaaagtagt tgaacttgca aattataatg
    26101 gaataccaca tctattgata ccagtagtta ctgaccctaa aaaagctgca aatgctttaa
    26161 attgggcagt tacagagatg aatagaaggt ataaattatt tgcagatgct caagtaaaag
    26221 atgtaacaag ttataatgag aaagcggaag aaaaacttcc taaaatagta ataataatag
    26281 atgagttagc agatttaatg atggcaagtg caaatgatgt agaagattat atttgtagat
    26341 tagctcaaat ggcaagagct gctggaatgc acttgattgt agcaactcaa agaccatctg
    26401 ttgacgttat aacaggggta ataaaggcta atataccatc tagaatagca tttgcagtgt
    26461 catctcaaac tgattcaaga acaatccttg atatgggggg tgcagaaaag cttctaggta
    26521 aaggtgatat gttattctat ccacttggtg cagcaaagcc agttagactt caaggggcat
    26581 tcatatcaga gagtgagtcg gaaaaagtaa tcgattttgt aaaaagtcag gttaaagatg
    26641 gaataaaata tgaagaagat ataattgaaa ctatatctaa ggttaatact tcaaagggta
    26701 gtgatgagga tgagtttttg agtgaagcga tagaatttgt cgtggaaagt ggtcaagcat
    26761 ctgcctctat gctacagcga agatttaaaa taggattcaa tagagcagct agacttatag
    26821 attctatgga agaaagagga ataattggag caagcgaggg aagtaaaccg agaaaggtat
    26881 taataagcaa acaggatttg cagaatttag aaggtgaata gaattgtcag ttataaaaat
    26941 agaaaaagca cttaagctag ataaagtaat atttgtagat gtgagaactg aaggagaata
    27001 tgaagaagac catatattga atgcaatcaa tatgcctctt tttaaaaata atgaacataa
    27061 tgaagttgga acaatttata aaatgcaggg aaaacatgaa gcaatacaaa aagggtttga
    27121 ctatgtatcg tataaattaa aggatatata tttacaagca gcagaacttg cattaaatta
    27181 tgataatatt gtcatatatt gtgcaagagg tggaatgaga agtggcagta tagtaaattt
    27241 attatcatca ttaggtgtaa atgtatatca acttgaaggt ggatataagg cttatagaaa
    27301 ttttgtttta gagtatttaa ggcatgtaat ggatacaaaa aactttgtat ctgttcatgg
    27361 attgactggt acaggaaaga ctgatttgtt acatttatta gatgaaaaag gaatagatgt
    27421 actggattta gaagggatag ctaaaaacag tggttctacc tttggcttta taacttttga
    27481 taaaaagcca ccatctcaaa agaactttga aacaaaatta tttgaaagta tatttttttc
    27541 gaaagagaat tatatatttg tcgagagtga aagcaaacga gttggaagtg ttacagttcc
    27601 aaatgaaatt ttcgaggcaa tgacacgaga aggacatcat atattattag aatgtgatat
    27661 tgaaaataga gttgatagac tttgtagaga ctatatttat gataaagatg aagggaatat
    27721 attgatttta aaagaatgta taaacaagtt tagaaaaaga ttgggtaata aagtagtaga
    27781 tgattatatt gatttattag aatcaggtga atataaagaa cttgtaaaaa gatatctttt
    27841 agaatactat gacccacttt atatgcattc agtagagaaa tataagtatg ataagattat
    27901 aaattttaat gatacacaaa aagctttaga agatgttata gacttatatg agagtatatt
    27961 ggaaggagaa aatgaatgtt aaaaatagca ttggaatcat taggatgctc aaaaaattta
    28021 gtagatgcag aaataatgat gggaatttta aataacaaag gctataaatt aattggagat
    28081 tttgaagaag ctgatgcaat tatagtaaat acatgtggat ttatagagtc agctaaacaa
    28141 gaatctatag atacgataat aaactttgca gaattaaaaa aaactggcaa tttaaagttg
    28201 ttgatagtaa caggatgtct agcccaaagg tattctgaag aattaaaaac tgaaattcct
    28261 gaaatagatg ctatagtagg tacaggaagc tatcaaaata tagataaaat acttaaagaa
    28321 ttaagtgaaa tgcatcaaat tgtaagcctt aatgatatag agtttgtatt taatgaagat
    28381 ttaccaagat atatatctac acctagctat atggcctatt taaaaattgg agaagggtgc
    28441 tccaataatt gtacttattg tataatacca aaactaagag gtaactatag gagtagaaaa
    28501 tttgaagata taattaaaga agctaagaaa ttagctgaat ctggagtaaa agaacttgtg
    28561 gttatagcac aggatacgac aaagtatggt tttgatttat acggaaaaga aagattatca
    28621 gaacttttag aagaacttgc aaagatagat ggatttaagt ggataagggt tatgtattca
    28681 tatccagaat ctataacaga ggaattgata caagttataa agaaatatga taatatatgt
    28741 agttactttg atatgccaat acagcatgca agtaacaata ttttaaaatt aatgaataga
    28801 aaaactacta aagaagatat attaaataaa atcaatttga taagaagtaa tataccagat
    28861 gctatactta gaactacgat aatagtaggg tttcctggtg aaacagagga tgacttcaaa
    28921 caacttgtgg actttgttga agaggttaaa tttgatagac ttggtgcatt tgcatattca
    28981 agagaagaag atacaccagc agatagattg ccaaaccata tagatgaaga agtaaaaata
    29041 caaagaagag atactttaat gatgattcaa caaaaaatat ctgaagaact taatgacaaa
    29101 aagataggaa aaacttatga ggtgttaata gaagaacaga tagaagataa tgtatataca
    29161 ggaagaacac aaggtgatgc ggaagaaatt gacagtatag tgtatgtgaa aagtgtagac
    29221 aatcttgaag taggagaatt tgtaagtgtt caaataaatg atgcaatgga atacgattta
    29281 atgggagatg ttttatatga acttgcctaa caagttaact ttgtttagaa tatttttaat
    29341 accagtattt gttttagtta tgttgttcaa tgtaccaaat aaatttttaa tagcttgtat
    29401 tatttttata atagcttcaa ttactgatgc tttagatgga aaaatagcta gaaaatacaa
    29461 tctagtcact gactttggaa aatttatgga cccattagca gataagctat tggttatttc
    29521 agcacttaca tgtatgatag aagaccatct tgtaagtagt tggatggtaa ttataatagt
    29581 ggctagagag cttacagtta gcatattaag agctatagct gctgctgatg gaaaagtaat
    29641 cgcagctgga aacagtggaa aacttaaaac gataactcaa atggtatcga tagtgttttt
    29701 attattaggt gctcagtttg aaaatggatt aatattaaat ataggtggag ttcttattct
    29761 tatagctacg ctacttacat tgtattctgg atgggaatat ctatataaaa ataaagaact
    29821 ttttatgtct tctaagtaaa aaattatgct ttaataaata aaaactaa
//
LOCUS       DAEMAE010000020        29465 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEMAE010000020 DAEMAE010000000
VERSION     DAEMAE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.020"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..235)
                     /locus_tag="KPH93_000766"
     CDS             complement(<1..235)
                     /locus_tag="KPH93_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG1687602.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNSIYVIDF
                     LDKNNLDNLKGNFYLIYQPHIEFEYLKNIFYNLF"
     gene            complement(265..1374)
                     /gene="ugpC"
                     /locus_tag="KPH93_000767"
     CDS             complement(265..1374)
                     /gene="ugpC"
                     /locus_tag="KPH93_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG1687603.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGMVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRIHIFDKETTLAIR"
     gene            complement(1527..2690)
                     /locus_tag="KPH93_000768"
     CDS             complement(1527..2690)
                     /locus_tag="KPH93_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1687604.1"
                     /translation="MKRAYKIEINPTTEQKSKIHQTIGVSRFIYNFYIAHNKEVYDSK
                     GEFISGMDFSKCLNNEYIPNNQDMKWIKDISFKATKQAIMNGDKAFKDFFKKTKGFPK
                     FKKKKNQDVKAYFPKNNKTDWTIERHRVKIPTLGWVRLKEFGYIPINSIVKSGTVSQK
                     SDRYYVSILVEEDDIQVSKCTNEGIGIDLGIKDFAICSNGSKFKNINKTSTVKKVEKK
                     LKREQRKLSRKYESLKVRNKNIKEGVATRQNIQKQIVKVQKIHQRLANIRTDYINKTV
                     SQVIEQKPSYITIEDLNVSGMMKNKHLSKAISSQKFFEFRTKLTAKCKQNNIELRVVD
                     RWYPSSKTCSQCGEVNKGLKLKDRVYKCECGLSIDRDLNASINLKNAKKYKIA"
     gene            complement(2662..3185)
                     /locus_tag="KPH93_000769"
                     /pseudo
     CDS             complement(2662..3185)
                     /locus_tag="KPH93_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            3499..4236
                     /gene="cas6"
                     /locus_tag="KPH93_000770"
     CDS             3499..4236
                     /gene="cas6"
                     /locus_tag="KPH93_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG1687605.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            4241..5707
                     /locus_tag="KPH93_000771"
     CDS             4241..5707
                     /locus_tag="KPH93_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687606.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEEIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVNTVIKSN
                     IEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKRSGQ
                     VESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKSFYK
                     KLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREPGNT
                     KNLGKKMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWIVDG
                     GEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRAQLFQTIMH
                     VLVSNGILIGEGFVEGIMQSSELELNTNVGIFIQEIMK"
     gene            5727..6740
                     /locus_tag="KPH93_000772"
     CDS             5727..6740
                     /locus_tag="KPH93_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG1687607.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRKLHQEYGWNLSNIIFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNRFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            6756..7553
                     /gene="cas5"
                     /locus_tag="KPH93_000773"
     CDS             6756..7553
                     /gene="cas5"
                     /locus_tag="KPH93_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG1687608.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            7580..9988
                     /locus_tag="KPH93_000774"
     CDS             7580..9988
                     /locus_tag="KPH93_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG1687609.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCVLHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFDEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILSEIYKSQGNLE
                     KNNIKFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKSILNKIIKTNSIFTMGIRKIILADYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            10429..11376
                     /gene="prmA"
                     /locus_tag="KPH93_000775"
     CDS             10429..11376
                     /gene="prmA"
                     /locus_tag="KPH93_000775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG1687610.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKR"
     gene            11395..12153
                     /locus_tag="KPH93_000776"
     CDS             11395..12153
                     /locus_tag="KPH93_000776"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG1687611.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            12155..13453
                     /gene="mtaB"
                     /locus_tag="KPH93_000777"
     CDS             12155..13453
                     /gene="mtaB"
                     /locus_tag="KPH93_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG1687612.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLREIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            13496..13846
                     /locus_tag="KPH93_000778"
     CDS             13496..13846
                     /locus_tag="KPH93_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG1687613.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            14009..14188
                     /locus_tag="KPH93_000779"
     CDS             14009..14188
                     /locus_tag="KPH93_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG1687614.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            14218..14661
                     /locus_tag="KPH93_000780"
     CDS             14218..14661
                     /locus_tag="KPH93_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG1687615.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            14772..15269
                     /locus_tag="KPH93_000781"
     CDS             14772..15269
                     /locus_tag="KPH93_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG1687616.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            15462..16025
                     /gene="raiA"
                     /locus_tag="KPH93_000782"
     CDS             15462..16025
                     /gene="raiA"
                     /locus_tag="KPH93_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG1687617.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            16240..16476
                     /locus_tag="KPH93_000783"
     CDS             16240..16476
                     /locus_tag="KPH93_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG1687618.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            16489..17832
                     /gene="yqfD"
                     /locus_tag="KPH93_000784"
     CDS             16489..17832
                     /gene="yqfD"
                     /locus_tag="KPH93_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG1687619.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKKNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            17771..18886
                     /locus_tag="KPH93_000785"
     CDS             17771..18886
                     /locus_tag="KPH93_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG1687620.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            18943..19404
                     /gene="ybeY"
                     /locus_tag="KPH93_000786"
     CDS             18943..19404
                     /gene="ybeY"
                     /locus_tag="KPH93_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG1687621.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            19410..20129
                     /locus_tag="KPH93_000787"
     CDS             19410..20129
                     /locus_tag="KPH93_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG1687622.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            20248..20649
                     /locus_tag="KPH93_000788"
     CDS             20248..20649
                     /locus_tag="KPH93_000788"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG1687623.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIHKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHNIKDLLPNYFSGNDLK"
     gene            20713..21606
                     /gene="era"
                     /locus_tag="KPH93_000789"
     CDS             20713..21606
                     /gene="era"
                     /locus_tag="KPH93_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG1687624.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARKDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            21618..22997
                     /gene="mgtE"
                     /locus_tag="KPH93_000790"
     CDS             21618..22997
                     /gene="mgtE"
                     /locus_tag="KPH93_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG1687625.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            23070..23225
                     /locus_tag="KPH93_000791"
     CDS             23070..23225
                     /locus_tag="KPH93_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG1687626.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            23191..23964
                     /gene="recO"
                     /locus_tag="KPH93_000792"
     CDS             23191..23964
                     /gene="recO"
                     /locus_tag="KPH93_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG1687627.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            23957..24556
                     /locus_tag="KPH93_000793"
     CDS             23957..24556
                     /locus_tag="KPH93_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG1687628.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMIAN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            24716..25594
                     /gene="glyQ"
                     /locus_tag="KPH93_000794"
     CDS             24716..25594
                     /gene="glyQ"
                     /locus_tag="KPH93_000794"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG1687629.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            25594..27660
                     /locus_tag="KPH93_000795"
     CDS             25594..27660
                     /locus_tag="KPH93_000795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG1687630.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIISSNVTKGHRFLGKSTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLRDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(27825..29372)
                     /locus_tag="KPH93_000796"
     CDS             complement(27825..29372)
                     /locus_tag="KPH93_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG1687631.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMVRLRVSAGVLSQHQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEAFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKEKGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRMLEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDDILNK
                     ITDKYKI"
BASE COUNT        11713 a         3105 c         5067 g         9580 t
ORIGIN      
        1 caaataaatt ataaaaaata ttttttagat attcaaattc tatatgaggt tggtaaatta
       61 aataaaagtt gccttttaaa ttatcaagat tatttttatc tagaaaatca ataacataaa
      121 tagaattatt gaaagtaact aaatggctac tagcatctaa cttttggttt tcatatggat
      181 agatatttat ttttttatta agttgtcttt ctaaaaaatc ctttaactct tccataaagt
      241 ctccttaaaa ataagattat attcttatct tatagctaaa gtagtttctt tgtcaaatat
      301 gtgtattcta ctgttatcaa ctgaaagtgt taagtcatca ccaacattaa ttctgttttt
      361 actgtcaaat ctaattgtta tagtattttc gcctagtttt aagtgagcat aagtttctgc
      421 tcccataagt tcttttattt caattcttga tttaaaacaa gtatctggat tagatttaat
      481 aaaagtatct tctacatgaa tatcttctgg tctgatacct ataacaactt cattattaat
      541 ataattgtct tttattaaat catagtgttt ttctttattc aatttaagct taatatgttc
      601 attttgtgca taaatttcat cattttcttc tattaatatt acatcaataa aattcatctg
      661 tggtgctcca ataaatcctg caacaaatat attagctgga taatcgtata catcttgtgg
      721 agttgcaatc tgttgaacca ttccatcttt cattactacg attctatcag ccatagtcat
      781 agcttcaact tggtcgtgtg ttacatagat aaatgtagtt ccaagctgtt ggtgtagctt
      841 agttatctct gttctcatag cagttctaag ttttgcatct aagttggata gaggttcatc
      901 cattaagaat accttaggat ttctaactat tgcacgtcct aaggcaaccc tttgtctttg
      961 tcctccagat aatgttttag gttttttatt taaaagaggt agtaagtcta atattttagc
     1021 agcttcttta acttttttgt ctatttcatc tttaggcaat tttctaagtt taagtgcaaa
     1081 tgccatgttt tcgtaaacag ataagtgggg atataaagca tagttttgaa aaaccatagc
     1141 tatatctcta tcttttggtt ctatatcatt gacaagttta tctccaatgt atagttctcc
     1201 ttcagagatt tcttcaagac cagcaatcat tctaagtgta gtcgatttac cacaaccaga
     1261 tggtcctact aaaacgataa attctttatc tttaatgtct atgtttatat tttttactgc
     1321 attaaatcca tttgagtaaa gttttgatat attttttaaa attacttctg acatagtatt
     1381 ctgctactca aaatatggtc aaatatatcc atatcgagat ttttccttct tcattgaact
     1441 tcgccttgcc taagctacta cgatttggta tagttctcca aaggcttaaa ttccgaagtt
     1501 agccctccgt acatatataa gtgcttttaa gctatcttat attttttagc atttttaaga
     1561 ttaatacttg catttaaatc tctatcaata gataatccac attcacattt gtacactcta
     1621 tctttaagtt ttaaaccttt attaacttct ccacattggc tacaagtctt tgatgatgga
     1681 taccatctat caactactct aagttctata ttattttgtt tgcatttagc agttagttta
     1741 gttctaaatt caaaaaactt ttgacttgat atagcctttg ataaatgctt atttttcatc
     1801 attccactaa catttaaatc ttcaatggtt atatagcttg gtttttgctc tattacctgg
     1861 gatactgttt tattaatata atcagttctt atattagcta atctttgatg aatcttttgt
     1921 actttgacta tctgtttttg gatattttga cgagtagcta ctccttcttt tatattttta
     1981 tttcttactt ttaaactctc atattttctt gaaagttttc tttgttctct ttttaatttc
     2041 ttttcaactt ttttgacagt tgaggtcttg tttatatttt taaatttact cccatttgaa
     2101 catattgcaa aatctttaat tcctaagtca atacctattc cttcattagt acatttagat
     2161 acttgaatat catcttcctc aactagtata gatacatagt atctatcaga tttttgactt
     2221 actgtaccac ttttaactat tgaatttatt ggtatatatc caaattcttt tagtcttacc
     2281 caaccaagag taggtatttt gaccctatgc ctttcaatag tccaatcagt tttgttattc
     2341 ttcggaaaat acgctttaac atcttgattt ttcttttttt tgaacttagg aaaaccttta
     2401 gtttttttaa agaaatcttt aaaggcttta tctccattca taatagcttg ttttgtagcc
     2461 ttaaaagata tatctttaat ccatttcata tcttgattat taggaatata ttcattattt
     2521 aagcatttag aaaaatccat accgcttata aattcacctt tactatcata aacttcttta
     2581 ttgtgagcaa tatagaagtt atatataaat ctactaacac caatcgtttg atgtatttta
     2641 gatttttgtt cagtagtagg attaatttct attttgtatg ctcttttcaa cttcttcatc
     2701 ctctctaatc tttttcttgt attttcttaa tccatatatg cgacaactaa atatttgtca
     2761 tatccaaatc gaatgaatct atctttatga gttacaataa tcgtatttac ctcattagtc
     2821 atacaactat caattagttt attccacttt ttacgattat aatttagtcc acttccataa
     2881 tcttcaataa cttcatctac tattattcct ttagcatttg catattgttt aagaaactcc
     2941 acttgatttt tcaaatcatc tttttgatta gaaattgaaa ctcttgtata aataacattt
     3001 tttctattta cttgttcttt atgtattcct ttatattcta aatattgttc ataagtgtaa
     3061 tatcttctat ctgttggagt tctaaaagct tttaatttcc cttcaatatc ccaacgttgc
     3121 agagttctgg ctgaaatatt aattaactct gcaaactctt ttggtttaca gttattgttt
     3181 ttcatataat tatctcttga ctataaacag ctatatttgt ttatgttttt attaactaaa
     3241 gataagctcc tgaatgttta aattttaatt atatagtatc aaattttttt attatattaa
     3301 aggtgtaaaa tgactaaata tatttgttta atatgtatag caggttttta gaaaagaaca
     3361 aaacaaacag tacaatatat ttaaaaaaat taaaaaacaa aattaaattt attatcaaaa
     3421 aatcatttta tgatatgttt tatttttata ttacaaatag agaataatga attatactag
     3481 aaagtaggag gtgaaaatat gagcagaatt tcaattttta tggaatcatg taatggcaaa
     3541 tttccaaatg aaaattcaat gttgtcagtt tcctttataa aaaatatttt aaatgcagaa
     3601 aacaaagatt ttgctaaaaa catatttaac tatggggaag aaagaaaaaa caataagcaa
     3661 ataaaggata taaatacagc catttacata ccaaatctta taagaagcaa aaatgagtta
     3721 cttgttgatg gagatataat attcaatata agtttttata attattctat gttcagtaaa
     3781 ttatacaatg gaattttaaa agtaaaggag ctagaatata aaggttatag atttaaaata
     3841 aaaaatataa aaatacataa agaacatgag ataaaagaaa atggtgttat atttaaaact
     3901 atgtctccta taatagtaaa gaataaagaa ggtaaatatt tagatgtaga agattcaaat
     3961 tatatagaat gtttaaacta tatagctaat ctaactctgg agagtataag aggtagtggg
     4021 cttagaaagc cattggaatt tatacctctt aatttcaaaa aaagagtgtt aaaagaaaag
     4081 attcgtggat ttaaagtgag agaatattac tatataaatg cttatgcagg aacatttttt
     4141 ttaaaaggaa atatggaaga tttaaatgca ctgtataaaa tgggaattgg atatagaagg
     4201 acggaaaatg caggaatggt tgatatatta aagtagcgag gtgagagaaa taaaagctaa
     4261 attttataga tcaggtgata ttgtaagaga tattggcatt gtttactttt atgaattact
     4321 aatggattta aaaaacgaac tagaaaaaaa tgattataag ctaaaattta cttgtgagtt
     4381 aaatagaaat tatttatctt tagaatctgc agaatatata gaccctaagt atatatctga
     4441 ttatatatta gaaaatcaac tttttaaggt atttaaaaat gggaaaaagg aaaccttaga
     4501 aaaaatattt agtaatatag ataatttaac gtatgtaaat tttattgaag agttagataa
     4561 atcttctgca actgagaaac aaaaggaaga gattaaaaaa gattttaaaa acagaagatt
     4621 tccatatgtt agaaatagtg gtaagtatgg attaaacacg agtctagaaa atatggaaac
     4681 aaatgtaaaa aggatagtaa atactgtaat taaatcaaat atagagatag ataaacttga
     4741 tttgacacaa tatgaagaaa aagatagtgt atgtaatgtt tgtaatataa ataaaacaac
     4801 aaaactagat attgatttaa gagaacgaaa ggactctaag tataactttt tatttagagg
     4861 tgcagaaaaa tcaggtttta aaagaagtgg acaagttgaa agtaatattt gttttgagtg
     4921 tgaatttttc aacttaatgt gtcttttata tataaattta aaaagaccaa tggtttttgc
     4981 atatacgaat gatttaagag aattagcatt tttaaaccat aagataatgc ttaaaagaaa
     5041 aatgtattca gataaatcat tttataaaaa acttttgcat gaaaaaataa gttcaattag
     5101 actatataga tttgatattg atacgaataa aggtataatt ctaaagtttg attctattat
     5161 agagtataaa gaattactaa aaaaaattga acttatagat attatagata attacaattt
     5221 ctcaagagag ccagggaaca ctaaaaactt aggtaaaaaa atgattgata atgggaattt
     5281 atcaaattta aaagaattat tacttgataa tataagtgtt ttaaagcaat caactggaac
     5341 ttctcgagaa atggattttg tatctagctc atataatatc ggtttatata taaaattgtg
     5401 ttggattgta gatggagggg aggagtataa aatgaaaaac tatatggaaa gtaatttaat
     5461 atattcaaga gtaggtaaag acttatttaa taaaatgaca gatgaatcca gaagaaaaaa
     5521 tttttctatg aaagtgattc aactacttaa gtctaatgat agagctcagt tatttcaaac
     5581 tataatgcat gtgttagttt ctaatggaat tttaatagga gaaggctttg tagaagggat
     5641 aatgcaaagt tctgaattag agttaaatac aaatgtaggt atatttattc aagaaatcat
     5701 gaaataaaaa agggggaaaa tttaaaatga atgaaaaaag agtaaaagga gtaacattaa
     5761 ctatactgac agaaagccca gttccacttt catatgacca agggtatgga aattatacac
     5821 caataaaaaa agaacaatat agagaaaaaa tacatgcaaa aactagtata gctacaataa
     5881 catatgattt aagaagaaag cttcatcaag aatatggttg gaatttaagc aatattattt
     5941 ttggaaaaaa aggaaatata tatccatcta ttaagaaaaa tgtaggatgt gtagatgaaa
     6001 atggattaga gactgatgta tttggttatt tgataccact taaagataaa ggttctataa
     6061 gtaaagcttc tccacttaga ataatacctt ttaggtcatt aaatccatat agaggttcaa
     6121 cacagttgat aacaaataga gggtttttaa gtagcgaatt tggtagaaaa tattatgatg
     6181 agaaggaaga aaatgaagta cttagagatg aaaactttcc aacaacacaa gcattggcaa
     6241 tagaagagac tttatcagat tactacgttt atacaataac attggagctt gatagaatag
     6301 gagtagtaga agtagaagat ggaaagttac ttttaccaga agaaagaaaa tttatgagta
     6361 aggagttaag agaaaaagca gtaaaagata tattagatgc aattactgtg tttacaagaa
     6421 atataaaaca ttcatcagta ttattaaaac cacttgcagt aatgggtgga gcttttgata
     6481 aagttgtacc gtttttttgg gatgatgtag attataatgc tgatagtggt gagataaatt
     6541 tagagggagt tatagaaact atagaaagtt atagccttaa agaaagtaat acaatacttg
     6601 ctatcaatga cagattaaaa atttctaata aaaaggaatt aaataggttt aatttaagta
     6661 aataccctgt aaaagagatt aaaaatctag cagatagatt ggaaattgga gaagataata
     6721 tgtggtattt aaaagagtag gaggtttttt gaattatgaa agtattacaa tgtaaactta
     6781 agcaaccaac tgctcactat agagacccaa aagtatttca aaatgaatat ataagtactt
     6841 taaatttacc atctaagacc acaataatgg gaatgataac gtatttatgt gatagaagat
     6901 taaactctga tatagatata ggaataattg gaacacatca tcatagagaa ctagaatttt
     6961 caagaggaga gaacattgat ttttggaatg aatatacaaa tatgagaaaa ggcaaagata
     7021 aagaaaagtt tttgcttaaa ggaaactact atgattacta taaagaacat aaagctcaaa
     7081 atagcatatt aaactatgaa gttcttaaag aagtagaact gactatattt atttcttgca
     7141 aggatgatga agaacttgaa tttattagaa aaaagcttga aagtccatgt aaatatgcaa
     7201 atttaggaag aaaagaagat tttgtaatac caagtgaaaa agggtgtttt gtgaaagaag
     7261 tcgagttaaa agaggttata cctatgaata ctcgtgatgc tataaaagaa aatataaaat
     7321 taaagaatac atatgtaaga gttgacttaa gagataaaga gaatgtcgag actataataa
     7381 atcaaggtgt attgattgca ttgcctcata aatataaaga tttagaagca aatagagatg
     7441 atagagttta tgagttttgc cattatatat atgttgataa tgatggtata tatcctaaaa
     7501 atattaaggt aaatgtatgt attgatacaa aagaagtttt tacatggctt taaattattt
     7561 tgaaagatag gtgtttatca tgaaaaatat catgcaaaaa gaaatattag ctaagtcata
     7621 caatttaaga actaatcaac aagagcaaac attagttgaa catattaagg atttatttga
     7681 agtattagaa tctatattag aactaaatct atattcagat aaagatgtag agatactgaa
     7741 aatatgttgt gttttgcacg atttaggaaa aattaattca attatgcaac aaaaaatgga
     7801 gattaataat aaaatatcgt atagttgttc agaagaagag aggaaaaagt tagagtctga
     7861 caaaaaatct ttaaaaaaga tagcaagaca caatatattt tctggagcat ttttaaaaga
     7921 tatactagaa aaaatgaact tgtcagaaga agataagtta tatatatata aaagtataat
     7981 gttacatcat ggaaactatg aagattatat gaggttaagt actagtaaag ttcaagaaga
     8041 aatttatgag tatattgaaa aaggcatttt agaaaaagaa gagtttaatc tgaaagatat
     8101 agaaagttat ataaatgaca ttttaaatgt agattttaaa tttgatgaag atgtattgga
     8161 ttatgattac atagataaat taagtgaaag tatgtttata gacagtgatt ataacaatgg
     8221 tcaaatagat gattcagttt taaattatag aaagtttaag tatattttat ataaagggat
     8281 gctgaactta atagaccata gtgcatcaag tagacagaaa ggtataaaat tttataacga
     8341 ttttactgat gaagaaattg ataatatgat tttaagtgag atatataaga gtcagggtaa
     8401 tttagaaaaa aataatataa agttcaatac tatacaaaaa aggttgagag aactttctgg
     8461 aagaaatgtt ctgactgttg catttacagg ttcaggaaaa acagctgcag attatagaaa
     8521 gacttttaaa agaaaattct ttttagtacc aaataaaata tcagcagaaa gtttttatag
     8581 aaaaaatatt tttcaaaata aaaatgattt agatacaaga agtagtaatg attacattgg
     8641 attacttcat ggagatataa acttatattc agagaatgaa gataatggag aacatgattt
     8701 tgtattgaca ttaagggata tagatttgag tataaacttt tgtaaacctt gtgtaattgc
     8761 aacagttgac cagttattac ttagtatgtt taaatttccg ggatatgaaa aaatatttgc
     8821 agccgtaaaa aatgcatcta taactgttga tgaagtacat ttattggaac caaagatgtt
     8881 tttgattttg atatatttta tgcagtttgc atgcaagtac ttagatgttg attttcactt
     8941 aatgacagct acacttccaa aatcatataa agagcaaatg ataaacaagg gaattatttt
     9001 tcaagaagaa agtaatgaaa atgttactga taaaggagaa atagttttta ttgagagcaa
     9061 caaggaagaa gagatttgtg aaggtaaaga tgtaaaggtt tcttttataa aggaaaaaga
     9121 aatcaagtca atagttgaag atgctttaga aaataaacaa aaaattctta taataaagaa
     9181 tactattgat tcagtcaata gaacatatga gtttttaaaa gaaaatttat ctgataagta
     9241 tggtggtgta gatatagatg ttttacatag tagatttaag ttcaaagata aaaaagagaa
     9301 atatagtaaa atattaaatg gagagggtga tatttggata tcaactcaat cagtagaaat
     9361 atccttggat ttggatttca atataataat aagtgattta gcaactatgg atagtttgat
     9421 acaaagaatg gggagatgca atagaaataa taagtatgaa tatggaaact tttatattct
     9481 acctagtgaa gataaaattt atgacaacaa acttaagaac accactaaga gtatcttaaa
     9541 taaaataata aaaactaact ctatttttac aatgggaatt agaaaaatta ttttggctga
     9601 ttactatgat aatagtgtag tcaaaaaata ttttgaagag aattttataa gttgtgataa
     9661 agaaattaag aatatttatg gtataaataa agaaattttt gatggattgg atttaatttt
     9721 taattttgaa ccttacaaaa atattgttga tagtaagaag gaagcagcta aaatttttag
     9781 agatgttgat gttagttaca aaattattct ggaagaagat ttttataagg aagataggga
     9841 gttacaacaa gattcgattc aagtatcagg gtttatattt aatagattat attattatgg
     9901 gttaataagc aaagtagaag gatacatggt actaaaatct agtagtaagt ttgagtataa
     9961 tagtactgta ggcttaattt tgaagtgagc atatgtttaa atataatata cctttagaca
    10021 ttgaaaatac aaaaagtact actgtaagct cactgtaaaa actatttttt taaaagaaaa
    10081 ataagttgtg agatagagtg tttacaagtg tatatagata ataaatttga ggtttaagat
    10141 taactaagtg ggtttcaggt acatcacgaa aataattata agttggagat tttgtatgtt
    10201 taagattaac taagtggatt tcaaatattg atatagtaga tataatagaa gttttattat
    10261 taggtttaaa attaactaat cggattttta aatattctga ctattatata aggatattat
    10321 tagcttgaat ttaaaactaa taatataaaa caacaaaata ttatatttat gatataataa
    10381 aagttatgga aaaacaggaa tattactata gagaagagag gtaaacttat gaataattgg
    10441 atagaagtaa ctataaaaac aacaacagaa gcagttgaac ctataacgaa tatactatat
    10501 gaacaaggag ctggaggagc tgtaatagaa gacccaaaag actttttatt ccaaaagaaa
    10561 aatgaactag attgggatta tgttgaagaa gaagtattta aaaagaatga agaagatgat
    10621 gttcttataa aaacttatgt ttctgaagaa aaaaatgtga tggaatttgt cgaaataata
    10681 aagcaaaaag ttttaggatt aaaagatttt ggtatagata taggtgaagg aagtgtatca
    10741 ttggaccaag taaatgaagc tgattgggca aatgcatgga aagcatacta caaaccaaca
    10801 aaagtaggac aaagggtagt agtaaaacct acatgggaag attatgctat gcaagatggg
    10861 gatttaatca tagagcttga ccctggaatg gcatttggta caggtacaca tgagactact
    10921 agtatgtgca ttagagaatt agaaaagtac gtgaataaag attctaaagt atttgatatt
    10981 ggctgtggta gtggaatcct tgcaatagca gcagctaaat taggagcaaa agaagttgta
    11041 gctgtagatt tagatgaagt ggctgtaaaa gttgcaaaag aaaatgttct tgaaaataaa
    11101 gttgaaaaaa gtgtatctgt aatgcatggt aatttaactg atgtaataaa agataaagcg
    11161 gatgtaatag ttgcaaatat aatagctgat ataataaaga tacttgcaaa agatgttcaa
    11221 aactttatga aagaagatgc tatattcata tcttcaggta taattttgga taaagtagaa
    11281 gaagttaaag aaagcttaat tgaaaatgga tttgaaatag ttgaagttca aaaattaggt
    11341 gaatggtcag caatagtttc taagcttaaa agataaggga tatggtgacg tataatggat
    11401 agattttttg ttgaaaaaaa taatataaat ctacaagata aaacttgcac tatagaaggt
    11461 gaagatgtaa aacatatttc aaaagtttta agatgcaaac taggtgagaa acttgaaata
    11521 tgtgacaaga ataataatga gtatatttgt gagataatga atatagataa gagtatagtt
    11581 aatttagaga ttttagaaaa agttgatata aatagagaat cagaacttaa agtaaggcta
    11641 taccagggac ttccaaaagc tccaaaaatg gaaatgatac ttcaaaaatt aactgaagtg
    11701 ggcgtagaag aaataatatt agttcaaact aagagaagtg ttgttaaagt tgatgacaaa
    11761 aaagaagata aaaaatttga acgttgggaa agaataattt atgaagcagc aaagcaaagc
    11821 aaaagaggta agataccaaa attaagaggt gttttgagtt ttaaagaagc tttagaagat
    11881 atgaaaaaaa ataatgtaaa tatttgccct tatgaaaatg aaagaacagt ttcaataaaa
    11941 catgcactaa aaaaatgtga ttctaatata gatagtgttg gaatttttat aggtccagaa
    12001 ggtggatttt ctgaagaaga aatagaacaa atacaaaaaa ataattgtaa tgttgtatca
    12061 ttggggccta gaatattaag aactgagaca gcttctgttg tagcatcaac aatagctttg
    12121 tatgaactaa gtgatttagg aggagaaaaa taaattgaaa aaagtagctt tttatacact
    12181 tgggtgtaaa gttaatcaat atgaaacaga agctatgcta gaactatttg agaaagatgg
    12241 ttatgaacaa gtcaatagtg aagagtatgc tgatgtttat gttataaata catgtacagt
    12301 tactcatatg agtgatagaa aatctcgtca atatataaga cgagttaaaa agaagaatcc
    12361 agatgcaata atagccgttg taggatgtta ttctcaggta tctccagaag aaattttgga
    12421 tatagaagaa gtaaatttag tcatgggtac aaatgataga agaaagattg ttgaagaaat
    12481 taaaaagata aattcaagta agaaagttag tacagttgat gacataatga aagttaaagc
    12541 ttttgaagaa atagaaatca gccaaactaa tggaaagact agagcattta tgaaaataca
    12601 agatggatgt gacagatttt gtacttattg tatcattcct tatgcccgag gacgcgttag
    12661 aagtagagat attgatagta tagtcgatga agtgaaaaaa ttggctaata atggctataa
    12721 agaggttgta ttaacaggaa tacatgttgc ctcttatgga aaagatttaa aggataggga
    12781 tattaaactt cttgatgtaa taaaacaaat aaaccagata gaaaaaatag aaagaattag
    12841 gcttagttct gttgaaccaa ttttatttac agatgaattt gtaaatgaag ttttaaagat
    12901 ggataaagtt tgtcctcatt accatttatc acttcaaagt ggatgtgatg aaactctaaa
    12961 aagaatgaat agacgataca caactttaga gtacaaaacc attgtagata gattgagaag
    13021 taaaatgcca gatgtggcaa taactacaga tgtaatagta ggttttcctg gagagacaaa
    13081 tgaagagttt aaaaaaacgt acgagttttt aagagaaata gagctttcac aaatgcatat
    13141 ttttaaatat tcaccaagaa aaggaactcc agctgcaact atggaaaatc aagttgaccc
    13201 tcaaatgaaa cattttagaa gcgaacaact tttaaattta agtaaagtaa attttaataa
    13261 atttgcaact aaatttatcg gccgtgagtt ggatgtttta tttgaacaaa atatagaagg
    13321 caataaatat gagggattaa cttcaaatta tataagagtt gttgttgaaa gtgacaaaaa
    13381 tatacaaggt cagatattaa aagtgaaaat aaatgatgta aaagatgaat atgtagaagg
    13441 aattttgcta taacatgtag aaattataat atttataaga taggaggtgg aaagaatgga
    13501 ttgtattttt tgtaaaatag cgaacggaga aataccttca actaaagttt atgaagatga
    13561 tagagtctta gcttttaatg acttgaaccc agttgctcca tatcatatat tagttgtacc
    13621 taaaaaacat tatgatagtt taattgatat tcctgataaa gagatggaca ttgtgtcaca
    13681 tattcatgtt gttattaata aaatagctaa agagaaaggt tttgaccaaa ctggattcag
    13741 agtcataaat aattgtggaa gtgatggagg gcaagaagtt aaacatttgc actatcatat
    13801 tttagctggt aaaaagcttc ctaattacga agcgggacaa aactaaacta aattattgaa
    13861 aaattgagtt ccttcttgat aaaaatactt gataaaagta aaaatttagt ttataataaa
    13921 caaagtgtta ggttgtaatg catattatgc aaaaagaaca tagttacagt cccagcataa
    13981 ttgcgctatt ggagggaggg aaaaagaaat gtcagaagta agagttagag agaatgaaac
    14041 attagacagc gctttaagaa gattcaagcg tcaatgtgca atgtctggca tcatgtcaga
    14101 agtgagaaaa agagagcact atgataagcc aagcgttaag cgtaagaaaa aagctgaagc
    14161 agcaagaaga aaaaacgcta agaaatagta gtaaataaga ataaagaggt gaaggggatg
    14221 tcccttaagc aaaagttaca agaggatttg aaatcttcga tgaaaaacaa agatacagtg
    14281 agaaaatctg tagtaacttt aataagagct tctataaagc agtatgaggt tgataatagg
    14341 gtcgagcttg atgaagatgg aattatcgat gtaatagcaa aacaattaaa gcaacgtaga
    14401 gatgcattag ttgaatttga aaaagctgga agagaagact taataaaaga aacagaaggt
    14461 gaaattgaag ttttaaaaga gtacctacct caacaattaa gcgaagaaga gttagaagaa
    14521 attgtaaaat ctactatatc tgaagtaggg gcaacttcta tgaaggatat gggaaaaata
    14581 atgtcagtta tccaaccaaa agttaaagga agagctgacg gaaaacttat aaataagttg
    14641 gtaaaacaaa acttacaata gattgatata gcctagagag aaatctctag gctttttata
    14701 atttttggga ataacgagtc atatttgagc atatctataa tcaaagaaaa ttttgaaaga
    14761 aggggaaaaa tttgattata tcagtaaaag agattaggaa ttttttgata aacatattta
    14821 taccactaat aataggttac ttaagtagca tcttaacaat gattatcagt ggaacagata
    14881 tttcaaccta ttatttacaa ctcacaaagc caagctttgc accaccagct tttatctttc
    14941 ctatagtttg gactatattg tacatattga tgggtatatc ttcataccta atattaagaa
    15001 agggttacaa cttacctaaa gtaagagatg caatttttta ttatggatta caacttgtat
    15061 taaactttat ttggagtata ctattttttg gctttggact tagatttaca gctttgatag
    15121 atataataat aatgattttg gtttctataa taatgatttc gaagttttct aaaatagata
    15181 agagatctgg atacataaat ttgatttatt taatgtggct tgtttatgca ggatttttga
    15241 actattttat atggtttatt aataaataga atgtaaagat tttgtaaatt accctgtact
    15301 tttaataata tcataacaaa cgaaaaataa atagactatt aaaaataatc attttcatat
    15361 acaataatat taatttaaat acaataaatt tataaaaatt gtatttaaag attaacatat
    15421 taaagtatta gaattggaaa ggcttgaagg gagaggtttt tatgcaaata atagtatctg
    15481 ggagacaaat gaaattaact gacggaataa aaggatacgt agatggaaaa ttaagtaggt
    15541 tagaaaaata ccttgatcct gaatcagaag tgaaagtaac agtaagtgcg aaaaaggata
    15601 gacaaaaagt ggaggttaca ataattccta ttaacggtca aataataaga gcagaagacg
    15661 tagaagatga cttatatgca gcaattgata ttgtttgtga caaattaagt agacaagttg
    15721 tcaaatataa aactaaagtt aaagacaaag ttcaaaataa taagagcata agatttgaaa
    15781 atcttgactt tatagataat agcagtgaat ttgatgatta tgattatgat gatgaagaag
    15841 atgaaaatat agtaatagaa agaagaaaaa aatttaatgt aaaacctatg agttctgaag
    15901 aggcgatact tcaaatggaa ctagtaggac acaatttcta tatgtttaga aatcaagata
    15961 attttgaaat aaacatagtg tataagcgta aagctggtgg gtatggttta atagaacaag
    16021 actaataaaa aaattgtata cgtttagatt ttatataaga aaaaataaaa aggtaatcat
    16081 gttgattttt tatcaatatg attacctttt tattgtttaa ataatatatt agatattagg
    16141 tatttattat ataaaaattt taaaactaat ctgttacaac atcaataaag tgcataaata
    16201 cctatttcaa gcataaaatt aattaagggg gtaatgctta tgttagaaat atccactgat
    16261 ttacaagtaa atcaaccagt tattactgtt acaagtaata cgtttattag tatagaaaat
    16321 tatctatcaa tattagaata tgaagttgat ttaataagaa taaagactaa agttaaaaca
    16381 attaaaatat ctggagataa actttcactt aagtatatta ctgatagtga gataggtata
    16441 aaaggaatta tctacaatgt tgagtatgtt gattaggagg agatacactt gataagtttt
    16501 ataaggggat actatgttat agttgtggaa ggagtaggac tcgaacaatt tttaaatcat
    16561 cttataagaa atgggataag tgtttacaat gtaactagaa taaaaaatac taaaatggaa
    16621 tttcatatag atagacaaga tataaaagaa tttaagaatg tttatagagg aagtaagttt
    16681 gatataaagg taaagcaaaa aactggggtt ccttttatta tcaaaagagt ttataagcac
    16741 aaaggtatgt ggatttgtgc tcttgtttct ttatttttat tgatgtctac atcacagttt
    16801 gtgacagatg tatatataca atctcctgaa ggtataaaaa aagaagctct tagaaatgag
    16861 ctttacaagg ttggagtaag accaggagtc tataagaaga gtattgatag aaaagaagtt
    16921 agagaccata tgatgtcaaa atttaatgat gtggcatatt tatctatcaa tgtaaaagga
    16981 acaaatatat ttgttactgt tacaaaaaag gcagaatccc taaaatctac agaccaatca
    17041 aattattgta atgtaattgc tttaaaaaat ggtataatag aaaaggtaat ccctagaagt
    17101 ggtaaatcag ttattaaatc tggagacata gtccaaaagg gagatgtctt attaaatgga
    17161 gctaatacca aatcaatacc agaagtatgg gcaagtactt tttatgagtc cacaaaaaag
    17221 gcaagttatg tagatactgt aaataaaaaa actggagaaa agaaaaatat atatacacta
    17281 agtttttatg ataaagagtt tacaatgaga aaaaatataa aatataaaga ttatgttgtt
    17341 gaaaataaag aaaaaaaact atcaatagga aactatactt ttccaataaa gataaaaacg
    17401 agtacttttt atgaaacaaa aaaggttgaa gtaaaaaaga ataaagaaga gttaaaaaaa
    17461 gaattatctg aaaaagcttt aaaagagtta gaatacataa tcccagcttc ggctagaata
    17521 atagatgtaa aacataactt taaggttaat aaaaatatgt tagaatattt aataacagtt
    17581 caaactagtg aaaatattgc taaaatttat cctcttagta agtcagaagc agaaaggttt
    17641 ataaaggaag aaagtaaacc agatgaaggt gaagaggaag ttccttccaa tccagaaaaa
    17701 agaccattaa atgatataag aaatgagttt gatgaggata ataaggataa taaagaccaa
    17761 aacaattctg atgaaaataa tagtaatcaa aatagtaata atagtcaaaa taataatagc
    17821 aataacaact aataaaatta aacttaaaat cttaggagga ctaattcaat tgataataca
    17881 aaagaaattt acaatagcag atggtaactt tgaaagagag ttatttggca actttgatga
    17941 aaatgtaaaa ttaatagaga agactttaaa tatagatgta atacttagag aaggtaatat
    18001 aattttaatt ggagaagaaa aaaatgtaga ttctgcatta aaacttatga atgaattaca
    18061 ccaaactgta tcaaatggta agcatttaga taaacagagt atttcttatt cattatctct
    18121 tttgcttgaa ggaagtgaac aaaaaataaa agaacttgag gggacaattg tcataactca
    18181 aaggggaaaa gctgttcaac ctaaaacttt agggcaaaaa gaatatataa aacttataga
    18241 aaataatgat ataacttttg gtgttggacc tgctggaaca ggaaagactt atcttgcagt
    18301 tgctatggca gtaaaagctt ttaaaagaga tgaagttagt agaattatac ttacaagacc
    18361 agctgtggaa gcaggagaga gtctaggttt tttaccagga gatttgaagg acaaagttga
    18421 cccatattta agaccactat atgatgcctt atttgaaatg ttgggtgctg ataaatttaa
    18481 taagtattta gaaagaggta ctattgaggt tgcaccactt gcatttatga gaggtagaac
    18541 acttgataat tcatttataa tattagatga agctcaaaat actacttctg aacaaatgaa
    18601 aatgttttta actagactgg gatttggctc taaagctgta gttacaggag atgtaactca
    18661 aacagattta ccacaaaata agaagagtgg tctaatacaa gctacagaga ttcttaaagg
    18721 tgttccaggt attgggtcta taatgctaac agatagagat gttgtaagac atgagttggt
    18781 acagagaata ataagagctt atgagaagca cgacaaaaga gaagaattta aaaaagaaga
    18841 acgtaaaaaa gcgaaaatgc aggaaaagac tttcaaaaga aagtaaaagt gtagtaatat
    18901 agatatatag cattattaat gaataaaaag taggtgtagt gaatggattt gatactagat
    18961 gatagacaag ataaattaga agtaagtgag gagttaattg aaaagataaa ggatataatc
    19021 atagaatgtt tagattatga aggatatgat gataattatg aagtgagtct ttcttttgta
    19081 gataataaag agattcatga attaaataga gaatatagag gagttgatag agtgactgat
    19141 gttttatcat ttcctctttt aagtgatgat tttgaggatg ttgagttaga agaagaatcc
    19201 ctaggtgaca tagtagtatc tcttgaaaga gccttagaac agagtataga gtataaccat
    19261 agttttgaaa gagaagtatg ttttttaata tgtcatagta tgtttcatct tttaggttat
    19321 gaccacgata cagatgaaaa tacaaaagaa atgagagaaa aagaagaaca catattaaat
    19381 aagttaaaca taacaaggga gtagttttta tgaaaccaga aaaaactcgt caaggtataa
    19441 ttaaagcatt taatgcagct atagaaggga tattgtatac atttaaattt gaaagaaata
    19501 tgaaaataca ttatttaggt tcagttgctg ttcttataat aagtttgttt tttaactttt
    19561 caaaattaga aatgataatg ttacttatgt ccatatgttt agttgtagtt gctgaaatgt
    19621 ttaatacagc tatagaaaaa gctgttgatt tagtaactga tgaatatcat gttcttgcta
    19681 agatagcaaa agatgttgca gctggaggcg ttctagtggc agcactaaat tcagtagtag
    19741 ttggatatat tttgttttat gataaattga cagatatatc tggaatatta atttataaaa
    19801 taagagaatc agaattacat ataaccttga tatgtatatt attggttctt atagctgttg
    19861 tagttgtaaa agcattgact tctacaggca ctcccctaaa aggaggaatg ccaagtggtc
    19921 atgctgcctt agcatttgcc atagcaactg caattacact tatgactgaa agagttgttg
    19981 cctctactct tgcatatata atggctgtat tagttgccca aagtagaatt gaaggaaaaa
    20041 tacatacatt ttgggagact attgctggag ctttattagg tgtgttgata gctatattag
    20101 tatttcagct tggaatgttc tataattaaa ataaatgtac ataaaatatt taaaataaaa
    20161 caaatctgtg ctaattaaat ttttatgata taatgtagag tattaaataa aaataaattt
    20221 taattattgt atacaggagg agttgttgtg gataacatag aattgttaag gttggcagaa
    20281 gatgccagac aacattctta tgctccttac tcaggtttta gagtaggtgc agctctttta
    20341 actaagagtg gtaaagttta tactggatgt aatatagaat gtgcatcatt aggtggaaca
    20401 aattgtgcag aaagaacagc tatttttaaa gcaatatcag aaggtgataa ggatatccat
    20461 aaaatagcca tagcaagtga taactcagaa aataatgagc aaacatatcc atgtggaata
    20521 tgcaggcagg taattataga gtttggttct gatataaaaa taataacggg atatactaaa
    20581 ggagaggtgt ttgagcataa tataaaggat ttgttgccaa actatttctc tggaaatgat
    20641 ttaaaataaa tttaaaagta taaataaatt taagtaatta aaacaagaag ataaaaagtt
    20701 aggagacagt taatgttcaa atcaggtttt gtcagtatag tgggtagacc aaatgtagga
    20761 aaatctactt taatgaataa tgtagttgga gaaaaaatag ctataatgag tgataaacct
    20821 caaactacaa gaaatacaat acaagcagta tatacagatg aagaaatgca gatagtattc
    20881 ttagatacac caggtattca caaaccaaaa aataaattag gtgaatttat ggtaaaggca
    20941 gcaacagaag cctttaaaaa tgtagattta atattatttg tagttgatga ttctaaaaaa
    21001 ataggtcctg gagatagaaa gataatagag gatttaagaa gtgttaagac accaataatt
    21061 ttagttgtaa acaaaataga ccaactagac caaaaagatg agctatttga tataatcaaa
    21121 atgtatgata gagaaggtat ttttaaagaa atagtaccaa tatcagcatt aaaaggaaag
    21181 aatacggata ccttaataaa agttatacaa aattatttag aagaagggcc aaagtatttt
    21241 ccagattata tgattacaga ccagccagaa agagtattaa tagcagagct tataagagaa
    21301 aaagttcttc actatcttaa tgatgaaata cctcatggtg tagctgttga aattgaaaaa
    21361 atgaaagcta gaaaagataa ggaaatagta gatgtatctg ctgtaatata ttgtgaaaga
    21421 gattcacata agggaataat aataggaaaa aatggtagga aactaaaagg aataggtaag
    21481 tcagcaagac aagatataga attactttta ggttcacaaa taaatcttca attatgggtt
    21541 aaagtcaaag aaaattggag aaatttacaa aattatataa ataattttgg atataatgat
    21601 aaataaaggt gggttatatg gataggaaac tggatgcttc ccaagattta ttatatgaag
    21661 tcaaatcatt aattgataat aataaagtat tggagttaag agaattaata gaggaatatc
    21721 atataattga tatattcgat ataatggaaa atttagagga agacatgaaa attcaattat
    21781 ttgaagtact tcctttagat atggcatctt ctatattaga agaaggtagt gtagagtttt
    21841 ttattagtat tttatctaaa ctggatgtag aacactcgaa aaatatactt gaacttatgt
    21901 ctcttggaga tatggcagac aagttgagtg aacttgaaga agaagaaaga gaacacataa
    21961 taaatctttt gaatcaagag aatgccgatt atgtaaaaga gttactattc tatgatgaag
    22021 attctgctgg tggaacaatg accactggat atatttctat aaataaagat atgacagctc
    22081 ttgaggctat agaccatatg agagaagaag ctgaagaagc tgaaactatt tattatatat
    22141 atgtggttga tgatgaagaa aaactagttg gagtattatc attaagagaa ttaataatag
    22201 ctagagatgc aaatatagta gaagatttga tgagtgaaaa tataatctct gtttatgtag
    22261 atgaagatag agaagaagca gtaagattag tatctaaata caatttaata gcaattccag
    22321 tagtagatag acaagaaaaa ttaaaaggaa taataacagt agatgatata attgatgtaa
    22381 tggaagaaga agcaactgaa gatatgtata aatttgcagg aagttcagaa catgaaagag
    22441 aagttgctga aaaagaaaac cctacattga gagagcaaat aatatcagca cttagaggaa
    22501 gattaccttg gttaattatt accttagttg gagggttgtt ggcttcgtta atactttcta
    22561 atttagacta tataatgaat cctgtatatg ctccattagt attttttata cctgtagtaa
    22621 ttggtatggg tggaaatatt ggaactcaat catcatctgt tacagtaata acattatcta
    22681 ataaggattt aaattttagc aatgtggtta gagaaggtat tgtaggtatt attacaggtc
    22741 ttttgtgtag tataataaca ggtattgtaa tttactttgt tatgagaaag ttagacattg
    22801 tattaattgt ttcaatatca ttatttataa atatggtttt aggtgcaaca ataggtgcgt
    22861 ttatgcctgt tttattaaaa aaaatggatg cagacccatc tacagtttca tcaccaatta
    22921 tatctacagc tcttgatata actggtatag cagtttactt tataataaca actgcattat
    22981 tgtcaaaaat tgtttaataa ctttatgtat gatttatcct cttcttacta aaaatataag
    23041 tataaatgta agaagggggt tttttaaaaa tgaacaatat cagttgggaa gtttttaaaa
    23101 aaacaggtag tatagatgca tatttatatt tctgtgattg caaaaacata catgaagaaa
    23161 ttgaagaagt aagggagaaa aaagaagacg atgataatcc tgaacaccca ggggatagta
    23221 cttaaagcta taaggtataa ggaaagtgac ataatactta cactatttac aagaaagctt
    23281 ggaaaagtat ctgcaatagc aaaaggagct aaaaaaaata agagctcttt gctttcatca
    23341 tcacagttat tttcttatag caattttacc ttaaaaaaac aaggtaatat gtacaaggtt
    23401 actcaaagtg aaataataaa aagtttttat aatatatcct atgatataga ggcattttct
    23461 tatgctacat atattacaaa attagttgaa aattctatac ttgaaaatca gactaataat
    23521 aggctattta tattacttgc acaaactctg tatttgtata cacaggataa tacagacaat
    23581 aggtttataa cagcagcatt tgagttaaaa tttttagact atataggatt taaacctata
    23641 gtaaataaat gcattaattg tgaatgtaaa attttacaaa attctgtgtt taatatctac
    23701 gaaggtggta tactatgtag taagtgtagt aaactttttg ataataatat aaaattagac
    23761 ttaacaacta taagtcttat ggaatatgta ttaagaaatg atattttaac atgtagtaaa
    23821 gctaaagtat caaaatatat aacacatgaa cttgagaata ttctagacaa gtatttgaaa
    23881 gtttatgttg ataatattaa ttttaaatca ttacacgtat tgcaaagtgt aaaaaataat
    23941 aagggagtgg ataatgatga gtagtaatat aactattgaa atggttgatt cagtctttga
    24001 aagagttcca ggagcaactt atgcagaagc aaaagaagca ttagtaatgt gcaatggaga
    24061 tgtaattgag gcagtaatat atttagaaaa taaaaaaaat acttgtaatt gtaaagcaaa
    24121 atctgctaaa gaaactatgg aagaagtatt tggcaaagat ggagaaaata taaagtttca
    24181 attaaaagag cttcttagaa aaagtagtgt agtaagaata atagttgaaa aagatggaaa
    24241 aactatgatg aacattcctt taacagttgg agttgttgga ttagcttttg taccattagc
    24301 tactttagtt ggattatcag ctgctgttat aagtaaatat cgtataaaag ttcaaaatga
    24361 agaagatgga gaaattgtag atttaggtga attgaatgaa gaaaaacttc atgttttgaa
    24421 gaatatgata gctaatgcag caaaagatgt gaaggatgta gtagttgaca aaaaagaaga
    24481 tgacaaagat gtaactgctg accttatgaa agaagaatct gaaaagaata aagaagacga
    24541 agacgaaaat aaatagatat aaagtgactt ttaaaaaatg aagagaagat aattataaga
    24601 attatcacta acattgacaa attttataga atttgatatt ctaaaagttg tagaagaact
    24661 attaaaatag cctttcgtgt agacgagagg ctttattgtt ataggaggga tttttatgaa
    24721 ttttcaagag atgatactgg cgttgcaaaa atactggtca aagcaaggtt gtatcatgat
    24781 gcaaccttat gatattgaaa aaggtgcagg aactatgaat cctaatactt ttttaagatc
    24841 attaggacct gagccttggc aagtatgcta tgtagagcct tcaagaagac cagcagatgg
    24901 aagatatggt gaaaacccaa acagactata tcaacatcat caattccaag ttatactaaa
    24961 accttctcca gataatatac aagaattata tttggaaagt ttaaaagaga ttggaattga
    25021 cccaagtgag catgacataa gatttgttga agataactgg gaagctgcta cagttggtgc
    25081 ttggggactt ggatgggaag tttggctaga tggtatggaa ataactcaat ttacgtattt
    25141 tcaacaagta ggtaatattg aatgtgagct tgagacagga gaaattacat atggattaga
    25201 aagattagct atgtacattc aagaagttga tagtgtttat gatttaaaat ggaatgataa
    25261 aataacttat ggtgaagtat ttaataaagc tgagtatgaa aactcaatgt acgcatttga
    25321 attatgtgat gcagatatgt tgtttaatct gtttgatata tatgaaaaag aagcattaag
    25381 acttatggaa aatggacttg taataccttc atatgactat gtgttaaaat gctctcatgc
    25441 atttaataca ttagatgcaa gaggtgcaat aggagttagt caaagagctt cttttatagg
    25501 tagagttaga aatatggcaa agactgttgc agaaactttt gtaaaacaaa gagaagaaat
    25561 gggattccct cttttaaagg acggtgacaa ataatgaaaa attatctttt atttgaaatc
    25621 ggtgttgaag aaatgccatc aagatttgta ggcagtacac tagaacaatt aaaaaacaat
    25681 cttagtaaat tatttgatga aaatagaata gcttttgata aaataaatgc gtatgggaca
    25741 ccaagaagac ttactttagt agtcgaagga ttgagtgata gacaaagtga tttagaagag
    25801 gaaataaagg gtcctgctaa gaaaattgct gttgatgcag aaaataattt aacaaaacca
    25861 gcactaggat ttataaaaag caaaggatta agcgaatctg acatatattt taaacaagtt
    25921 ggaaaagaag aatatatatt tgcgactata aagcaagatg gaaaagaaac ttcagaagtc
    25981 ctaaaagaca tattaccaga gactataaaa tctgtagtgt tcccaaaagc tatgcgttgg
    26041 ggaggaaaaa atcttaaatt tgcaagacct ataagatgga tagttacatt gttaaatgat
    26101 aaagtgttag agtttgattt agaaggaata atatcttcta atgtaacaaa aggtcataga
    26161 tttttaggta aaagtacaat agaagttgat tctatagaag attattttga aaaattagaa
    26221 aaaaattata tagttttaga ccaaaacaag agaaaagaaa tgataagaaa acaatgtata
    26281 gaagtagcaa attctttggg tggagaagtt gaatttgatg aagatttact tgaagaggta
    26341 acttacttag ttgaatatcc aactgctttt tatggagaat ttgatgtgga ttatataaaa
    26401 ctaccaaaag aagttgttat aactcctatg aaacaacatc aaagatattt tccagtactt
    26461 aaagaaggaa aactgttacc taactttata gctgttagaa atggtgattc ttacagaata
    26521 gacaatgtta aagctggaaa tgaaaaagtt ttagaagcaa gacttgcaga tgctttattt
    26581 ttctatagag aagatactaa gagaaactta gaaagctaca tagaaaaatt aaaaactgtt
    26641 gtattccaag taaaactagg aactatttac gacaaaactc taagacttga aaaattaagt
    26701 gctgatatac ttgatagttt aggtttagta gatgaaaagg cagatactat aagggctgct
    26761 aaattatcta aggcagattt agttactgga atggtaggag aatttgatga attacaaggt
    26821 tttatgggaa aagaatacgc aaaagttgga ggagagaatg acgttgtaag tgaagctatt
    26881 tttgaacatt atcttccaag atttgcagga gatatacttc ctaagactaa tgcaggggtt
    26941 gccttatcaa tagctgataa gttggattca atagcaggtt tctttgctat aggaatacaa
    27001 ccaacaggtt ctcaagaccc atatgcttta agaagacaag ctttaggtat acttagtata
    27061 cttatggaca gaaaagttgc tgtagatata aaagtactta ttgaacatgc tttagataat
    27121 tattctgaat tagattttaa taaagaagaa gttgttgagc aaataatgaa tttcttcaat
    27181 gagagagtaa aaaatctatt taaagattta ggaataagat atgatgttgt tgatgctgtt
    27241 ttaagttctg atataaatga tgtttctgat atgcatatga gagctttaga gcttaataat
    27301 tggctagaaa aagatgaatt agtggagatg cttactgcat ttaatagagt ttctactcta
    27361 gctcaaaaag cagaaagcga taaaattgat gaatctttat taaaagaaga tgctgaaatt
    27421 aaactttaca atgaatttaa acaagtaaag gtaaaagtta atgaattatt aagggataag
    27481 aaatatggta ttgctttaga tgaatttgca tctttaagac cagtagttga taatttattt
    27541 gatacagtta tggtcatgga taatgatgaa gctatcaaga acaatagact tgctttactt
    27601 aaacaagtat atgatactat gctagaaatt tgtgatttat ctaaaattgt ttataaataa
    27661 agtttaaatt atttataaga aataaggctg tctcaaaata gagatagtct tatttttcat
    27721 tgttgagatg atgaaaatat atttgatttc atagaaaaag agtgtctcat aaactaaaaa
    27781 gttcattttg agacaccaaa ctttttgtga gtaaatataa taaattatat tttatactta
    27841 tcagttattt tgtttaaaat atcatcattt gaatttaccc attcataaaa attgcctgat
    27901 gatgcatccg atattttata aagcatttca ggaatatcac tagctttaaa atctccaagt
    27961 gattttccta atctagtctt acctatttca aaactgcctc caacaaataa ttcaaatgca
    28021 tctgtagata taccatcaac attcttcttt ttaccgcata aacctatact accaacttga
    28081 tgaacgccac atgaatttaa acatcctgat atatataatt taggtgcttt atttaaaatt
    28141 tcttcattat tttgtaatct aaaataatct atgatttcat gtaacatttt ctgactattt
    28201 tgtataccca tttgacaaat tggaacacct atgcaagata tactttgttc taattgagaa
    28261 ttacaactaa tagttttact tatttctaac attctttttg cttcatttcc atttaaattt
    28321 aaaatatata atccctctgt cattcctagt ctaatcatag gattttcaat attatctaat
    28381 tctttaagta aagtacttaa atcctttgtt aacaaaatac ctccaactgg atgtatatac
    28441 acagtgtata aaccatcctg tttttgaggt attagtctag aatcctgtat atctatttta
    28501 actccaggtt tagagtagtc tatttcttca atattgagtt caagactacc cttttctttt
    28561 tctaatttat agtattcttt aaatttttct aagaataatt cttcaccaag tgattcgacc
    28621 atatatctaa ccctagctct atttttattt tcataattgc cataatccat aaagaattta
    28681 atcatacctt caacacaata taatgcatct ttcggcttta taagttcatc aagttctaaa
    28741 gcaactttgg gttgtttacc tagacctcca cctaagaaaa ctctgaaata tggttcatca
    28801 tttttcaaag tagcaacaaa tcctaagtct tgtatactac aatgagcagt atctgtgtaa
    28861 caattagaat aagaaacttt taattttcta ggtaaatggt atgtagtagc atttttgata
    28921 aaatatttat cagtagcaac tgcatatgga gtaacatcaa atgcttcact agggtcaact
    28981 ccagataatg gagataatcc gacatttcta ggataattac caccaccacc tctagtgaat
    29041 atatcatttt ttattccttc ttccatgata tcaacaattg aatttataga taaatcgtgt
    29101 aattgtatag cttgtctagt tgttaagtgt atcttatcta agttgtgttt aatagctatt
    29161 tcatagattt tattgagttg atgctgagat aaaactccag cagatactct aagtcttacc
    29221 ataaaagatt ttttgtctct ttgagcataa acaccatatc caccagaaaa tcccttatat
    29281 tgcattttat ttatttctcc atttaaaaat tttgaagaat attctttaaa attaggtagt
    29341 tcatttaaca atatcttttt aaaattatcc attatttcac ctcattttta gtcttgtata
    29401 aaaatatatc tattgtataa tagatatata tattattatc tagtcaggat aaaaatataa
    29461 cagaa
//
LOCUS       DAEMAE010000021        28879 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEMAE010000021 DAEMAE010000000
VERSION     DAEMAE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.021"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(140..334)
                     /locus_tag="KPH93_000797"
     CDS             complement(140..334)
                     /locus_tag="KPH93_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687632.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(643..2172)
                     /gene="lysS"
                     /locus_tag="KPH93_000798"
     CDS             complement(643..2172)
                     /gene="lysS"
                     /locus_tag="KPH93_000798"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG1687633.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKDEE
                     N"
     gene            complement(2187..2666)
                     /gene="greA"
                     /locus_tag="KPH93_000799"
     CDS             complement(2187..2666)
                     /gene="greA"
                     /locus_tag="KPH93_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG1687634.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2775..3743)
                     /gene="dusB"
                     /locus_tag="KPH93_000800"
     CDS             complement(2775..3743)
                     /gene="dusB"
                     /locus_tag="KPH93_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG1687635.1"
                     /translation="MKIGNLKLKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYSGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3880..4650)
                     /locus_tag="KPH93_000801"
     CDS             complement(3880..4650)
                     /locus_tag="KPH93_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG1687636.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4669..5259)
                     /locus_tag="KPH93_000802"
     CDS             complement(4669..5259)
                     /locus_tag="KPH93_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1687637.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYMSGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5442..6410)
                     /locus_tag="KPH93_000803"
     CDS             complement(5442..6410)
                     /locus_tag="KPH93_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG1687638.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKAIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIIKKREI"
     gene            complement(6403..7380)
                     /locus_tag="KPH93_000804"
     CDS             complement(6403..7380)
                     /locus_tag="KPH93_000804"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG1687639.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLETIDSTNEHAKRTAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYIVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLPRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNKELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7702..9672)
                     /gene="ftsH"
                     /locus_tag="KPH93_000805"
     CDS             complement(7702..9672)
                     /gene="ftsH"
                     /locus_tag="KPH93_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG1687640.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEIE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9706..11094)
                     /gene="tilS"
                     /locus_tag="KPH93_000806"
     CDS             complement(9706..11094)
                     /gene="tilS"
                     /locus_tag="KPH93_000806"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG1687641.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIVFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11277..11705)
                     /locus_tag="KPH93_000807"
     CDS             complement(11277..11705)
                     /locus_tag="KPH93_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG1687642.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNEVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11722..12528)
                     /locus_tag="KPH93_000808"
     CDS             complement(11722..12528)
                     /locus_tag="KPH93_000808"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG1687643.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12730..13215)
                     /locus_tag="KPH93_000809"
     CDS             complement(12730..13215)
                     /locus_tag="KPH93_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG1687644.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTEKIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13266..13925)
                     /gene="spoIIR"
                     /locus_tag="KPH93_000810"
     CDS             complement(13266..13925)
                     /gene="spoIIR"
                     /locus_tag="KPH93_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG1687645.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14070..14750)
                     /locus_tag="KPH93_000811"
     CDS             complement(14070..14750)
                     /locus_tag="KPH93_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1687646.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14734..15624)
                     /locus_tag="KPH93_000812"
     CDS             complement(14734..15624)
                     /locus_tag="KPH93_000812"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG1687647.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWKLRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTISKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15742..17292)
                     /locus_tag="KPH93_000813"
     CDS             complement(15742..17292)
                     /locus_tag="KPH93_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG1687648.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNREQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17386..17652)
                     /locus_tag="KPH93_000814"
     CDS             complement(17386..17652)
                     /locus_tag="KPH93_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG1687649.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17800..18660)
                     /gene="yabG"
                     /locus_tag="KPH93_000815"
     CDS             complement(17800..18660)
                     /gene="yabG"
                     /locus_tag="KPH93_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG1687650.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18744..19682)
                     /locus_tag="KPH93_000816"
     CDS             complement(18744..19682)
                     /locus_tag="KPH93_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG1687651.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNKLGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19792..19953)
                     /locus_tag="KPH93_000817"
     CDS             complement(19792..19953)
                     /locus_tag="KPH93_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687652.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19963..20460)
                     /locus_tag="KPH93_000818"
     CDS             complement(19963..20460)
                     /locus_tag="KPH93_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG1687653.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEDVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20575..21285)
                     /locus_tag="KPH93_000819"
     CDS             complement(20575..21285)
                     /locus_tag="KPH93_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG1687654.1"
                     /translation="MKNYEYKGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNNVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFDFKLDKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21508..21699)
                     /locus_tag="KPH93_000820"
     CDS             complement(21508..21699)
                     /locus_tag="KPH93_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687655.1"
                     /translation="MKKVLAHYHLKILSNNLEDISSFINYDIEDIIGCIEPSIKITLL
                     QKQKLSLYNELKKYLQTIM"
     gene            22219..22914
                     /locus_tag="KPH93_000821"
     CDS             22219..22914
                     /locus_tag="KPH93_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG1687656.1"
                     /translation="MLVDLNNSSSVFSDTQNNTSLESIYFNKWKGKSWITLGDSITRA
                     NGYQDKLKNILGFSRIDNMGENGQTMVSQSENKSTYTLGKTIDYKVYDLATIFIGTND
                     FRYNKKLGKIKVSGSIRFDETTFTGSYQLLIEKILSSNPSIDLVLITPPQRIRDGYDI
                     DFTNEVGSKLIDYVNVIKSLGEMYSLPVLDLYSEGGISKKNISTFTRDGLHPNDVGYD
                     RISDKMYRFLLSI"
     gene            complement(22928..24124)
                     /locus_tag="KPH93_000822"
     CDS             complement(22928..24124)
                     /locus_tag="KPH93_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687657.1"
                     /translation="MNNKKSKWISIPFVVIIIGVFLIHIVSKDKEISASENRTLAQKP
                     TIQDFKFEKYTSEFESYFSDQFPFREELSEIYSKVQIVLGKNKIKNYYLLDDNWIMPT
                     PSKSMSEKELKDAANEINELSKIASDSNKKVYYVSTPNKESMLAHLYPKYVDGLKNDI
                     SNKNAFKSYLDLETINVIDVDEHFLKEFNESQREDLYFKTDHHWNGIGAYEGFKCIIE
                     DMNIVNNISWDSYTQTMYDEGYFLGSYNKNLNKLVKEDETIPYVHLKEKPNYEYYKFD
                     GKTETKCKEEDVIATRKNEKEILYGGAYMFGNACSILKIRNEDALSDKKILIIRDSYQ
                     APTSWLFADIFSEVQLVDPRYIDKLDISIDDIIRDSKADLVMFMYNTSDFKMMIDVMK
                     EQREKS"
     gene            complement(24139..25554)
                     /locus_tag="KPH93_000823"
     CDS             complement(24139..25554)
                     /locus_tag="KPH93_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG1687658.1"
                     /translation="MIFSSLTFLFAFLPVVFILYFLVNDKLKNFVLLLTSLFFYAWGE
                     PKYIFLMLGSIVVNYIFGLKVSSDNKKEQKLWLTLSVIFNISLLVIFKYSNFFVDNFN
                     ALFNSHISIPTIALPLGISFFTFQTMSYVIDVYRKDGRVQRNIFDLALYVSLFPQLVA
                     GPIVRYQTVDEQISKRIYSNEKFAVGVNRFICGLAKKVILANQLGMVADGVFSANIAN
                     LSIAESWLGIICYTLQIYFDFSGYSDMAIGLGKIFGFDFLENFNYPYISQSVSEFWRR
                     WHISLSSWFRDYVYIPLGGNRVSPIKQYTNLFVVWSLTGIWHGANWTFLTWGIYYGIL
                     ISIEKVFLGKLLKKVPQIFRHIYLILIVMIGWVFFRADNIVQASEFVQVLFGVGSSPI
                     CNNSFIMYINDYGYIIILSIIFAIPIIPKLKSILKSKTKKLVESNFVYMLHSTFLVAL
                     MFIVVVILINSTYNPFLYFRF"
     gene            complement(26202..27455)
                     /locus_tag="KPH93_000824"
     CDS             complement(26202..27455)
                     /locus_tag="KPH93_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1687659.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGSSDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPESTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKNLNNKIA"
     gene            complement(27506..28660)
                     /locus_tag="KPH93_000825"
     CDS             complement(27506..28660)
                     /locus_tag="KPH93_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1687660.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKDAMKPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
BASE COUNT         8970 a         5295 c         2953 g        11661 t
ORIGIN      
        1 ttcgtcaata ctttttttgt tttttatttt aaaatatttt aaaaaaggtt ataacttttg
       61 ttttttttta ataactttaa ttaaaactaa tagatataac ccttgatatt acaaacaata
      121 catgcatata taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc
      181 tgactctatc tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa
      241 aaatatccat tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac
      301 aaaataaaca taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat
      361 atatagtatt ctcataaatt attcttttta ccttctcata aattattcta aatattttca
      421 taataaatta ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata
      481 tcaatttcta acgtttgttg gtaatttata aattaaatat ccaaaagaat ttttgaagca
      541 tataaaaagc attctacact gacattttaa ataaaattta gctatttatt gctaatttaa
      601 tagctaaact ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcatcttt
      661 atttggattg ttatcaatag gtttcatagt agggaataat aatacatctc ttatagatgg
      721 agagttagtt aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg
      781 tagacctacc tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc
      841 taattctctt tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag
      901 ttcagaaaat gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct
      961 tcttgggtct tcaacatttc tcttagataa tggagatact tcaactggat gatgagttat
     1021 aaatgttggt tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc
     1081 tcctcttctc attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc
     1141 ttcatctgta tttatagtgg aaaaatccac accagaatat tctttaacgc aatcttccat
     1201 actcattctt ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat
     1261 acttcctgta gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc
     1321 agtatagtct gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa
     1381 atccatacct tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac
     1441 aataagtctt tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt
     1501 attatgatta gttataaatg gtctagcatt tgctccacct gctatagtat ttaatattgg
     1561 agtctcaact tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt
     1621 tgttctaatt aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg
     1681 tctatatctt agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga
     1741 tttacatagt aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa
     1801 gatttcccct tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc
     1861 ttcctctcct atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat
     1921 atctataaaa ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt
     1981 agttttacct tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc
     2041 atatctactt tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct
     2101 tcttacttga agtacttcac taaggtcttc ttgaatttgt gcttcttcat tgctttgttg
     2161 attatttttc atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca
     2221 actccatcag gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttt
     2281 cctacaggag attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact
     2341 atagtatatt caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt
     2401 gtaacaacat taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt
     2461 tctaatttta aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca
     2521 gcattttcag ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt
     2581 ctctttacaa cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc
     2641 aataaaaatt ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa
     2701 ttttcctaat tatattttaa aaattttata atcttttttt aatttatgcg ctatattata
     2761 agtcactatt atttttatgt caaggagtct tccatatagt actccaattt cgacacaact
     2821 tcttgatagt cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct
     2881 tttaaatacc aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct
     2941 tctactgcaa gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct
     3001 ggcaaaatct cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct
     3061 tgagcacccc tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa
     3121 tcttctattg taaatacatc cccatttcct ataacaggta tactcaaatt tttcttaatc
     3181 tctgcaatta tatcccagtc tgctttacct gaataaaact gttctctagt tcttccatgt
     3241 attgctaaag cgcttattcc acaatcctct gctatctttg ctatttcaac agcatttaca
     3301 ctattatcat cccaaccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact
     3361 actgccctca atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca
     3421 tttttaacta cttttggagc tggacacccc atatttatat cgagaatttc atttgaataa
     3481 tcattcatta tctctgctgc tcttcccata aactctggct ctgagccgaa tatctgaact
     3541 gctacaggat gttcttcttc ttctatctta agcatttttt tagtattttc atcatcatag
     3601 caaagtgcct taccatttat catttctgta tataaaagcc cacatccctg ttctttacaa
     3661 attaatctaa atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc
     3721 aattttaaat ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta
     3781 actttaaatt cttatttcct cattataata taccacatat atctagaaat ctatcataaa
     3841 aagggaattg gatatataac ccaattccct gtatattaat tattcttggt ttttttcata
     3901 tattattctc aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc
     3961 tgaagcaatc agtttagcta atccacctgt agctataacc ttgacatcat cacaattcaa
     4021 ttctttcttc atcatactta ttattttgtc aactaaacca acatatccat aaattattcc
     4081 agattgcata gctgatacag tactcttaca aatagtcata cctggtttag ctaattctac
     4141 tctaggtaat ttagatgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc
     4201 tattgttcca cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc
     4261 aaaatcaaca agtatacttg gtgctccata cttttctatc cctgctacag cattaactat
     4321 tctgtctgcc ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc
     4381 tggacctact attaatggct gttttttaca gtattttata caaaagtttt caagagaatg
     4441 cataacattt ggaactacag atgatattat aacatcatca atatcactta tattcacatt
     4501 atcataatca aataggttac ttataaggat tccatattca tcagacgttt tttctctatc
     4561 tgttttaatt ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt
     4621 agtatttcca acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct
     4681 tttcttaagt acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac
     4741 tccatttgct gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc
     4801 atgaagatac ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa
     4861 cagagaacct attgcccctg taattaaacc tgacatgtat accttatttg tagccttaat
     4921 tattaactga tatacataat aagctaatac acctatcata actcttggta atattgaaac
     4981 taatggattt ataaaaacaa aagatgttac tgttgggttt gttatagcat ttaagagact
     5041 tgatatccca aatataaatc ctattgcagc cccaactatt ggtccttcca taatagctcc
     5101 aataataact ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa
     5161 tggagtcata gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct
     5221 tacatttact tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca
     5281 gctcattaag atgccggact acttaatttt ttaatttttt cgtttttttc ttataattat
     5341 gagtcaagct accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt
     5401 gccattgtca acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt
     5461 attatatctt tatatgccat tatattttct acactatccg ctttttttac tgcatttatt
     5521 atactttttt ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt
     5581 tctcctgttg atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata
     5641 gctttagcat atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac
     5701 ttagcatttg ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt
     5761 tcaccttgtt tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca
     5821 cctgctataa ctttaccatt ttcataaaca tctccaagag catttacagc aattaatgca
     5881 gatacaacta gaccattatc taattttata gaatagctac ctattccgcc tttcatagca
     5941 tattcctgac ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga
     6001 tttaaaatat cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta
     6061 catttaaagc tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta
     6121 cctacaccta catcaaatcc aattcctttt tcttctaagt atctacttac accacaagtg
     6181 gattcaagac catatgcaga acctccagcc aaaactactg catgaacttt ttggaccata
     6241 tttacagggt ctaataaatc ggtttctcta gttcctggag cagaccccct tacatcaaca
     6301 ccgcaagtag ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc
     6361 tcaacctgcc caacctttaa gccttctata tccaaaatat tattatacat atcctttttc
     6421 tcctcttata gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac
     6481 taataaattc ccctcttcat ttatgtctaa gcatttcact agttctttat tatctccttt
     6541 taatacatat atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt
     6601 ttctttggaa ttatttacag tataatctaa atataatttt tcaaattcag tcaatacatt
     6661 cctaacaata tctactctgg gtaacatata tccttcttta tacaatgatg tagctttgtc
     6721 agctatttct tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaatgta
     6781 gtttgttctt tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt
     6841 taaaataaca tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt
     6901 cgttacactt gctcctgcta ttaaagttat aaatggagct ttgaatggtt ccatatctgg
     6961 ttttaatata atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa
     7021 tcgtcctttc cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat
     7081 agctgtcctt ttagcatgtt catttgttga atctatagtt tcaagatgtt caatattttt
     7141 tccaataaat tctgtatcca atttatagta aatattctgt gggcttaata aatcagttgg
     7201 attttccgct aatcgatacc ctttcttatt tacagattct atattatatc cctcttcttt
     7261 taaggctttc atatgtttcc atatagctgc acgtgacact cctaattgtt ttgataattc
     7321 ttctcctgaa ataaattcag atttattatt taatataatc tctattattt tatctcgcat
     7381 atttcacctc taatatgaca tgatttcatg acttattttc ttctaataaa taattataca
     7441 ttattaatat acttttaata ctattaattt taaatttcct ttttaattat tataatggtt
     7501 gttttatatt ttatatttta tattgataat ttatatgatt ttacatttta tctaaataat
     7561 tttaaaataa taaaaaaact gaaagtatca attaaattaa tgcatgcaaa tatactttgc
     7621 ttattaattt aatattatac tttcagtttt agtatttaat ccacatcaga taataatctt
     7681 aaaatgacca aggtcttaaa actatttatc tttatcagat ttatcttcta aatttttatt
     7741 tatatcaata acattgtttc tttcttcttc atctttttct aattttatag ttaattgctc
     7801 ttcaatttct ttagaagaat tttcctctgt aactgcattt tcaatttcat tcagaggtaa
     7861 ttctttatta aatgctttta caaattgttc tgcatctaaa gtttcatata taagtaaagc
     7921 ttgtgctaca tactctaatc tatccatatt ttcttgtaat atacttcttg tcttatcata
     7981 tgcaccatca actatacgct ttgtttcttg gtctatctca aaagcaactt cttcagaata
     8041 atttctctta cttgaaaaac tattacctag gaatacttca tcatcactgt caaatgacat
     8101 tggaccaagt ttagaactca ttccatactt tgttaccata cttcttgcag tagctgtaac
     8161 tctttctaag tcatttgaag cacctgtaga tacatcctta agtactagct cttctgctac
     8221 tctaccacca agtaaaacta caatattttc tttcatttca ttttttgtag catagaactt
     8281 atcctcaaca ggaagttgca ttgtaaaccc tccagctctt cctcttggaa ctattgtaac
     8341 ttgatgaaca ggacttactt cttcaagtac atgggcacaa actgcatgcc ctccttcatg
     8401 gtatgctgta agtcttcttt ctttctcact tattactttc gattttttag ccacacctgc
     8461 tataaccttt gttatagctt cttctatagt ttccatctta attttctttt ctctttttct
     8521 cgctgttaat attgcagcct cattcattaa gttctctata tcagcaggag taaatcctgg
     8581 tgttcttcta gccaagacat ccatttttac atcatcactt aatggtttat ttcttgagtg
     8641 aactttaaat atagcctctc taccttttac atctggtgta ccaacaacaa cttgtctgtc
     8701 aaatcttcct ggtctaagta aagctgggtc aagtatatct ggtctatttg tagctgccat
     8761 gattattata ccttgattta ctccaaatcc atccatttca actagaagtt gattaagagt
     8821 ttgctctctt tcatcatgac ctccaccaag acctgcacct ctttttctac caactgcatc
     8881 aatttcatct atgaatatga tagctggagc acttttctta gcctgttcaa ataaatctct
     8941 tactcttgaa gcaccaactc caacaaacat ctcaacgaaa tcagaaccac ttatactgaa
     9001 aaagggaact cctgcttctc ctgcaactgc tcttgataga tatgttttac cagttccagg
     9061 aggtcctacc ataagcattc cttttggtat tcttgcaccc aattcaatat atttcttagg
     9121 attctttaag aaatctacta cctcctgcaa atcttctttt tcctcatcta gtccagctac
     9181 atctttaaat gtaactctag ttttttcatc atctttatga accttagctt ttgatttgcc
     9241 aaaactcata acctttccgc ctccaccttg agactgattc ataaacacga accaaataat
     9301 caccatgaag aatattaaca gtaatgtagg caacatttcg acaaaccaag gtgtagatgg
     9361 cttagcttca ccaccaaaag ttagtttccc agccttagct tggtctaaca cctcatctgc
     9421 aagtttattt cccatcactt ctgttggaac atatgactta aactttgtat ttgtatcttt
     9481 tatagtacct tctacagaag tttgatttac aaagtataat cttgatatat tctcatctgt
     9541 taattctcta tatacttcag agaattttaa atcttttatt ttttctgtag gcttacctga
     9601 gaattgaact atacctacta taattataaa aacaagtaga taaaaacctg ccccttttag
     9661 caacttattc aatatagaag ccctcctttc atacattgct ttgccctata atttgttaaa
     9721 attaattttt aatacttctt ttgtgttttc atcaatttta taattttcac tgattttata
     9781 atctccaaca catattattc cttcactatc tgtgattata ggaattttac atctttcttc
     9841 tcttgggatt tttaaatcta tgaataaatc tttcactttt ttacttccca tagcaagctt
     9901 tatcttatca ccttgccttc tgctccttat tactataccc ccttttacct tattaaaatc
     9961 aaacccttta gatgaattgt ctaatttcat actcttatac ctatctatgc tcattacttg
    10021 ggtctcaata atttgtttta attcttttat tttaataaac ccattactag gtacattgta
    10081 ataaaattca atttcctcat taacaatttc ctcattcgtt aatattatac tgtctttttt
    10141 tctataaaca aatatatttc ttggaagagt taacatttta tttacttttg aattatcttc
    10201 taaagacatt atatcctcta tatgtctttg gtcaacaaaa ttagtatctc ctagtatatg
    10261 ttttatggaa tttctaagaa ctctaacttt gatagcatca tgtaagcaaa caaaatcgtc
    10321 taaatttatc tctacaaagc ctttttcttt tatatttgaa acttctctaa atttagcttc
    10381 tgcctctttc tcaatataat cgttatcact ttttaaacta ttgctcatcc tcactataga
    10441 ttctattaca tttgaattaa aattatcctt catatatggt aaaaggtcta accttatctt
    10501 atttctagtg tatatatttt ctaaatttgt tttatctatc ctcggattta aattataagc
    10561 ttcacaatac tcctctatct catttctttc cacatctaat atgggtctaa ttatgcaatt
    10621 atctctaata taatcaattc cctttaaacc ttttaaacct gttcctctca tgatacgcat
    10681 cataacagtt tcggcttggt cattgagatt atgacctatt gctattttat tggctttaat
    10741 cttatcctta atttcgtaaa acatttcata tcttagcttc cttgctcctt cttctaatga
    10801 taatccctca ttttcacaat attttggtac attaatagac tttacaaaga atacaattcc
    10861 catatcctca caaagtttag atacatataa tgcatctttt tgagcttcta tccctcttat
    10921 ttgatggttt aaatgtgctg catatatttc tatattaaaa tcttttttta atctatttag
    10981 tacatgcaat aaacaaactg aatcaggtcc tccagaaaga cctaaaacta ttttatcgcc
    11041 tttttgtatt aaattatgtt tatttatagt acttagtacc ttatcaaata tcatatttat
    11101 ccctcttaca cttagtttta ttcactttta agtatacttt ctataaaaaa acgctaaacc
    11161 cttgtatcaa ttttaagaaa ttaaagttta atataaatat aataatacca aataaattaa
    11221 ataaaaggta gttttttcac tacctttata aaatttctgg aaaatagcca tagttattat
    11281 ttagtaccta tgtatatatc tattttattc ctacctaaaa ataaatcttg tacctttata
    11341 ttatagtcta ttttcaattt tatgtgttcc ccttcttgta tatttatatc caattctctt
    11401 gtatcaatat caattaataa agtaccttgt ggggtcacat actttgtagt actactttct
    11461 ccacatttaa acaacattgt tgagttaata gcaccaaact ttttgatact aacttcattt
    11521 ttaagtatct ttatagtatt agttatttct atattttctt ctttttcctt gtaaataaca
    11581 tatattccat catttttata aaaaatttta ccaaaggcag tcatctctat ggtgtccata
    11641 tttccttttt catcatattg tctagtattt atacttattt ttacactcaa cgatttttcc
    11701 tccatagatt cttcatataa actagtattt ttgtatttct actttttgaa tcttatcaat
    11761 ttttttcttt aagaactctc ttgctatatc cgaaaatttt tcaggtatat ctgatacata
    11821 atattgataa gttccatgaa tttctgtatt gtttataata ttttgcacct ctaaaatctt
    11881 ttttaaatct tttgcagtct ctttagctgg attaacaagt tttatatgct ctccaacttc
    11941 ttcacctatt gttctcttta aaataggata atgagtacat cctaacacta aagaatctat
    12001 atttttttct ttcaattctt gtaaatatat ctttgctgtc aatttagcca cttctgtatt
    12061 tgcccatcct tcttcaacaa taggtacaaa taaaggacat gctttattaa caatttctat
    12121 actctcatct attttagata tttccaagtt gtatgctttt gaacttatag ttccttcagt
    12181 tcctattatt cctacaattt tgttttttgt agaacttaca gctgttctag ctcctgcttc
    12241 tataactcca attatcggta tatcatattt ttcttgagct tcttttaaac ttcttgcagt
    12301 agcagtgtta catgctataa ctatagcttt tacacctttt gtttttaaaa agtttatagc
    12361 ttgaaaagta tatttaatta tagtttcttt ggatcttgaa ccatatggta ttctggcagt
    12421 gtctccaaag tatattatat cctcatttgg taatatcttc attatctctt ttagaactgt
    12481 taatccacct aatcctgaat caaaaactcc tattggttta ttgttcatct ttatacaaca
    12541 cctactttac atttttgtgt ttaacaacaa cttatttatc gctaaaattt atatttatat
    12601 tcataattaa tatattatta cctaaatgat tattatagtt tattttacac aaaaaataaa
    12661 ggagctatct taaattagaa aataatttct acaaaaagac aactccttta actacttaaa
    12721 aatttaaatt tattttgcaa acacatgatt tcctattctc atataaggat tcaatctatt
    12781 tatccatttg caagtggcta tttctggatt ccaaaaatat actgctttat tagttgggtc
    12841 agttccataa agagcttcct gagctgcttt acgtgctgag tctgttggtg ccatctttat
    12901 tgaaccatca agcacaactg agaaagcatt tttttgatat atgacttctt ctattgtgtc
    12961 tggaaaccta gggtccaata ctctatttat tactacagct gctacagcaa cctgtccctc
    13021 atagctttct cctctagctt ctcctgcaac caacttagat aatagcatca agtcattatc
    13081 agataaattt attacttctt tctttgctat atttttttct tgtttgttta ctttcttaat
    13141 cttttctgtt ggttcatatg caaatacctc tttaataggc ataaatataa aagtcaaagc
    13201 taatagtgta gccatcagtt tactccacct gtccatgatt aaaaccactc cttacattct
    13261 tatttttaaa atttcttctc taaatccttc actacttcaa gtactttaaa cttaattttt
    13321 actctacttg ttttttttgg tgtatcttgt tttattaact tatattcttt atcagttaaa
    13381 acttccttca acttatcatc tgtagattta tctattactc cattagactc atcaacaaaa
    13441 catagtggag ggaacataac acaccaccag ttttttcctt caccttttcc aatgataatc
    13501 tttaatgctt tatattcacc agctggaaga actatattag aatattgttt agtagggaaa
    13561 ttgctatatt gtatacctac ctttacactg taattatatc catttttaag aataatattt
    13621 ttacttattt cttccagact actatattcc ttttttataa tagcttcact ttcttttata
    13681 ctttttgaat tttgaagctt tggctgtaaa tattttatta cttcatctct aacttttagt
    13741 ttaagttctt ggtcttcatc tgtattgctg tttgctataa catgaaatct tattaattta
    13801 tctttataat cttttgatat attgtctact ttttttacct caccatttat tactattgtc
    13861 attattgata tgactgatat aagacttaat attaatagtc ccaatctaat cttaattttt
    13921 ctcattgaaa attccccctt aagataaatt tatatcatca attatgtcca attaaattta
    13981 atttattcat aatatttttt aatactaata aaaaaagcta tcttaaaatg attacaatca
    14041 ttttaaaata gctttttaaa ccttatattt tataagtctt tactaatcat agccttatct
    14101 atcataaact gctctgcttt ttctatatgc tctgtagcat acttcttgga tagctcacta
    14161 tcacctttaa ttatagcatc aagtaaatat ttatgctctt caacaatatt atgtgatgta
    14221 tcataagcag acatataggt aactctaaat ctatatactt gttcccataa tgagtttatt
    14281 agttggatta attttttatt attagttgct ttaaatatac attcatgaaa ttctacatca
    14341 agttttaata acgcatctac atcggattcc aaagtgcttt ttttaaattc ttctgatata
    14401 tctttaagct ttttaatatc ttcatcacta attctttcag cggcaagata tgctgctaag
    14461 ccttctaggc ttgctcttac ttctaataca tccataatat cctttaatga catatttgcc
    14521 acatacgcac cttttctagg caacataacg actaaacctt ctaattcaag ctttcttatt
    14581 gcctctctca ctggagtcct acttactcct aattgttctg ctagttgcac ttccattagc
    14641 ctttgaccag gctttaattt tccttctaag attgcttctc ttaaattctc aaagactaca
    14701 tctcttaatg gcttgtaatt atccaagttc agtttagtta aattctccac aaatttcaac
    14761 ccctttttga ctgctattaa caacatacac ctgtttatat ttttttaata attctttttt
    14821 tccaattaga gcatcttctt tatttttaaa tagtccaaaa actgtaggac cacttccact
    14881 catcatagaa cctaaagcat tatttttcat cattacttgt tttatatcac ttatttcttt
    14941 gtgtttactt atagtaacat tttcaagaat atttaccatg ctttctgata cagcttttat
    15001 atcttcagat tttaaacact ctatcaaata tttattattt ggcctttttt taatattatt
    15061 caaatctaat ccttgataaa cttctttagt agaaacaaat aagtctggtt tgcatatcaa
    15121 tatatttgta tcacatggta aacctttgat gtttgttagc ttttctccaa taccttgagc
    15181 taaggcaggt cttcctgata tacaaaaagg aacatctgca cctaaattaa gtcctatttc
    15241 ttttaattca tcttcagata atctaagttt ccataggtga tttaaaccta ctaatactgc
    15301 ggctgcatta gaacttcctc cagccatccc tgctgctaca ggaatatttt tttctatgaa
    15361 tatttctaca ccttttttta tataaaattt atttttaagt attttggcag ctttataaac
    15421 aatattatct tcatctaaag gaatatctaa actagtgctt tttaccttta tttcatcttc
    15481 gtctaattct tttatcttta ctatatcgta taaatctatc gtttgcatta tcatttcaac
    15541 aaaatgataa ccgtcttgcc tttttcctaa gacatcaatg gataaattta tttttgctct
    15601 actctttagt cttataaaat ccattctatc ctccttgttt aaagaataat gcatcactaa
    15661 caataatatt atactacaaa aaataaaata atagggtatt ataataccct attatactta
    15721 attactttaa attttttaat attaatctac taatacgact tttttttcta aaataagaca
    15781 cttaccaggc ttaattggtt catcaagttt tatatcatta agttctgcaa tttcattttc
    15841 tgttgtattg tatttttttg ctatattcca gaaagtatct ccttctttgc atatatatac
    15901 gattatgcta ggagctttag ataaatcata tacaccttgg tcttcgccct tgataataaa
    15961 attttctgct ttcttatcta atgcttcagt aaatctctta attttgataa tcaagtctat
    16021 ctgttcccta ttcaaatcaa cttcgacttt atcaatacat gctgtattaa acacagatgc
    16081 tgtatcagtt agattatcca tttctatatc atgttcaaat ggaatttcct cactaatttt
    16141 ataaactaat tttaaacctt caactgggac aaataaaatt tcaactttta tgattccttg
    16201 tataatgctt ttatttcctt caatataact attttctata gacatggttg gacaaacgct
    16261 tacaatatct tttatttgta tatcatcatt atcatttctt atgccttctc taaccataaa
    16321 tgtttcttca ctacgtctta aagtcttatt taattgaata ggtttgtggt caaattttat
    16381 tattttttga ggcgaatatg catcttgtaa tacttctctt gtaacttcat ctgtaacttt
    16441 taccttacaa caagccatac aatctatttc taaaagacct gtgttacttt cactattttg
    16501 cttgaaaata tgattaaaat ctgacataga caacaatact tcttcagtca taccatcact
    16561 aactccaggg acttctacaa attgggtaaa ctcaatacct actctatcca actctacaag
    16621 ttcaccctca tatgtacaag ctaacggatt tatttctaat actccaccaa taataacttt
    16681 attgtcagtc actctacttt cttttacttt aacacatgga tttaaactta ttatagattg
    16741 aatttcctct gtgtttattg ttatagtatc tcttatagaa ctttctgctt tttctattcc
    16801 aactatatct tgaaaacaaa tttcctttct gtgcttttgt atgccttcca cctgtgccac
    16861 atcttttaca atgtctaacc tttgcttttc aaacaaactt ccccttatat tcataagagc
    16921 tcctacttta atctttctct cattcattat agtacaatcc atatgctcta cttctgaaaa
    16981 taacatgtat tccatatctt gaacaacatt gtctttttct attacttcgt ttatatctac
    17041 ttttccgtct acattagaaa tagtattttt atcatctgca atatagatta cattataatt
    17101 aaaacttcct ctacaaagta tcttcccatc tgccatttca atttttttaa gtgatatgta
    17161 tccttctgtt tttacaattt catatacatc taattttttg tctggtacaa ctgcctccgc
    17221 ttcaatgaat gtttgaaatt ttccaaagtc tattctattg tcaactttaa ttacatcttt
    17281 aattaattcc atagtctacc tccttaagta ttataaaaat atcacactat atttatattt
    17341 aaggagcaaa aaaaaaatta cacctatttt taaataggta taattttaac ttatttgtaa
    17401 tttatcttga tctctaaaaa cttgcaactt tacattagat gttaataaat ctgagtaact
    17461 ataagatact ctaggatatc cattactttc atcatctaac tttataacaa acacgcttgg
    17521 ataaactttt tctagtatac cctcttttgt aataatctgc tttcttcctt tattagcttt
    17581 caacaatatt ttctttccta tatgtctctc taagcttact cttatcttat ctagagtttg
    17641 aacagtagcc acaatatcac cttctttaac gtttatatgt ttacataata tcacatataa
    17701 acatttttgt caaatgttta aactaattat tttatatatt ttttacaaaa atgtcaatac
    17761 tatttttgaa gatatacagt ataattatgt taatttttgc tagtgtaata ttgtttttgg
    17821 cattgcccat ctaaattttc ctcttgtttc agaaccacca attcccttaa cacctgtaat
    17881 agtctgctct ataacctcat caataggaac tactacatca attcttgaac aagcaatttt
    17941 ctccactata aaaacagggt ctaatgcatg tatcattatt cttccaggcg aacttgcata
    18001 gtttgctcct gcacttatta ttttttcata atttgattgg catgctcctg cgaatatgac
    18061 aagattatct aaatttggct gccatttgcg tgcttctttt actgttttaa caaaatttga
    18121 agagtttcta tagttactca tatcttctat atctcctctt tttattgtca ttgcatcatg
    18181 acctgttatt actagtatat ccggattata tttttctagt agtgctctta cttctttata
    18241 ctgattttgc tctgaaacag ctactcctac tgcaggtatt ccaagttgag catatacatc
    18301 caaacaaatt tttaaatatt ctttatctcc atctatctgt agtacttttc caggcattcc
    18361 atatgtgtta ggatttaatt gaagttttgg aactgctcta gtcattcttt tttgtctttc
    18421 tttagctttt cttactgact tatataataa attttctaca tttttatcta ttaatatatc
    18481 ttttatatca ggtgctttta ctaattctag gtcatcaata taagcatctg ctattactct
    18541 aaatgcaatt ccctttagta tagctatttt ctcattattt tcatctacac caaaagagac
    18601 tattttaaat actatatctt tgttatgtga ctttctagct acaatgtctc ctaccttcat
    18661 agttaaacct ccaataaatt tttatcttag tttatactat gttcgatata aaactttgtt
    18721 tcctaatgtc aaaaaaacat ttcttataat acttctttta tcactcttaa agcattttta
    18781 tagtatatct tttctatatc atcttcacta aacccttctt tttttagagg ttcatacagt
    18841 ttacccatct gagatatatc ttcaatctca acttttgaat ctattccatc aaaatctgaa
    18901 cctagagaca caacatctat tcctccaaca tttacaatat gctttatgtg tctaaccata
    18961 tcttcaagtt tactttcact tttatcactt aaaaataagt ggaagaagtt tatacctgta
    19021 actccacctt tatttgctaa taccttaatc atatcatcag ttaaatttct agaatgattc
    19081 atcattgctc ttgagttaga atgtgtagcg attattggtt tagaagaaat ctttgcaatt
    19141 tcataaaatc caccatctga aatatgagaa acatcaacca acattcccaa tttattcatc
    19201 ttatgtacta cttctcttcc aaaatcagta agcccttttt ctctatattc tactttatta
    19261 tgaggaaaac ttaattcatt cacatgattc caacttattg tcatcattct aacccctaag
    19321 tcgtaaaatt tctttaaatt ttctaattta ccttcaagca ctgcaccttc ctcaatagat
    19381 aaaagagctg taagtttacc ttctgattgg tttttcataa tttcttcata gttagtagct
    19441 aaagctattt catcactatt ttttttcatt tcctcatgaa atttatttgc catactcatg
    19501 caataatcaa aaggatgttt tacttcttca gtatcaacaa aaagagcaaa tgtttgagct
    19561 aatgaatccc cctttttcaa tctgtctata tcaacagaat atttattaga cttaagttca
    19621 cttgtttcta cattttccat taattttgct atagtatcac aatgtaaatc aataaatttc
    19681 atatttatac cccctgtata ttttatattt atatctaaaa atagaaatag ttgtccttat
    19741 agacaactat tgacaactat tttttaatat tctttttaat gttacttaat cttattcttc
    19801 ttttaagtta ggtattactt ttttaacttc ttcaatttga tgccatgttt tatctatatt
    19861 attttcacta atatattttt ttaaatcagt taaagatgta aaatctttaa ctgataaatt
    19921 gtcatcatca tctgcaaatc ttaaaataaa catgagactc ccttactttc cacaacattt
    19981 cttatatttt tttccgcttc cacatgggca tgaatcattt cttcctattt ttgcttcttt
    20041 aactattgtt ttagacttct tataagcctt tattatttct tttctttggt ctgctggtag
    20101 tatatcttcc caaccttgta aattatatag ccaatgagcc tcaacagtta ccatattgtt
    20161 atataatttt tcccaatcta tttttataga aacttcttta tcagcttcta catcttctaa
    20221 aactatatct tcttttaaac tttcacttat tccatctatg aatcccatga aaaattcatt
    20281 agtagttcca aatttttcag ctaattcact tactttacct tcaacaacat ccattttatt
    20341 atttaatatt tcattgtata ttccagcttc aactttcaaa tattcttccc agaattttac
    20401 ttctgcttct tgactttcat gattatcact taaatttctc cattcagtat ataaactcat
    20461 actttttcct cctaagtata cctatatttt tatagttttt atagtataaa atcttttagt
    20521 tagattggaa gtttcttctt tttgattata ccatattcta tttaaagtta actcttatat
    20581 tatatcataa ttaaacaatt cttttaatac ttttatagca tactcttggt ttataaaata
    20641 agggcttgtc gcaattgtga ttactttggc ttttttaatt aaatttttta tcttgtctaa
    20701 tttgaaatca aatggtatat agtccatatc acttgaaaat atatccaaat ctatatcaag
    20761 gacaaactct gactctactt ctaaatcaaa tccataaaga ctatctatta ttattaattc
    20821 tgaaaatatg ttatatttta aagcaggctt tataaaactt cccacattta aaacttcatt
    20881 tgtatatcta aatacatctt ttagattatt tacatctaca tcatagttct caggttctct
    20941 agtatcctta tgttggtcta catgcactaa cttgcatcct tttgtaaact caccctttaa
    21001 caaagatttt gcccaaaaat aaaatgcatg gttatgattg tcaaatacat atatcttttt
    21061 atttcccagg acatactcaa ccatattctc caatcctttt gcttttattt ccttattttc
    21121 atctacttca ttaaatacca cttcattgcc taaccttaca ttatttaatg taccatttat
    21181 aagttttgga acgtatatgc tcttattttc tctcttatca tatgaaaaca cattatttcc
    21241 tactggtttt tctatataaa atcctttata ttcataattt ttcataaaag ttaaattcct
    21301 ccattattat aatatataag ctaaattcac ctatttttgt attcttggtg actatcacta
    21361 atatcttctc tcaatgtgta ttatacaata agtataagat acatttataa attagtatta
    21421 tttcttgttt aaatataaac gagattatct caaaattgat tttttttaat caaattttaa
    21481 gttcaactca tttttgaatt tctgtattta cattattgtc tgaagatatt ttttaagttc
    21541 attgtataaa gataattttt gtttttgtaa aagtgttatt tttattgaag gctcaatgca
    21601 tcctattata tcttctatat catagttaat aaaactagat atgtcttcta aattattact
    21661 taaaattttc agatgataat gtgctaaaac cttcttcatt ttgtaataaa tatttgctaa
    21721 atcaaagtac tcacttgcag attctattat attttttgtt gaatttagat acttaattta
    21781 ttttattaat ttgtcttctt ctgttgagtt gttgcaattc ttattataga aagcaattaa
    21841 ttcaaaaaca aaatttaaaa ttacatcttt acgattttat atatcctgat ttgaatttgt
    21901 tccaaatact ttaatatatt ctaatttttg accaatatcc ttttctccac tttctgttaa
    21961 ttgttcaaca cttaaattat ctaatttttc atacttacaa atcaatcaaa gcaacatagt
    22021 tatatatttt attcaaattt tttataggta tatttagttt gagatatcca tgaattttcc
    22081 actttatttg catctaaatt atgttaagaa taatttatga atcttaatac ataataaaac
    22141 atataggagt ggtatcatat gaaaaagagc accatatgtt ttttattagt tacaatggtt
    22201 agctgtatta cttcatatat gttagttgat ttaaataatt catcatctgt atttagtgat
    22261 acacaaaaca atacatcctt agaatcgata tattttaaca agtggaaagg caagtcatgg
    22321 ataactctag gtgatagtat aacaagagct aatggatatc aggataaact taagaatata
    22381 ttaggattct caagaattga taatatgggt gaaaatggtc aaacaatggt atcccaatct
    22441 gaaaataaat caacctatac acttggaaag acaattgatt ataaggtata cgatttggca
    22501 acaattttta ttggaactaa tgattttagg tataataaaa aacttggaaa aattaaagtt
    22561 agtggaagta ttaggtttga tgaaactacc tttacagggt catatcaact gttaatagag
    22621 aaaatattgt catcaaatcc tagtatagat ttggttttga ttactccacc ccaaagaatt
    22681 cgtgatggat atgatattga ctttaccaat gaagttggaa gtaaactaat agattatgta
    22741 aatgttataa aatctttagg tgagatgtat tcgcttcctg tattagattt atactctgag
    22801 ggtgggatat caaaaaagaa tattagtaca tttacaagag atggtcttca tccaaatgat
    22861 gttggatatg atcgtatatc agataaaatg tatagatttt tattaagcat atagcaaaga
    22921 gaaaagatta actcttttct ctttgttctt tcattacatc tatcatcatt ttaaaatcgc
    22981 ttgtattata cataaacatg actaaatcag ctttactatc tcttataata tcatcaatag
    23041 atatatcaag tttatcaata tatctaggat ctactagctg cacctctgag aatatatccg
    23101 caaataacca tgatgtagga gcttgatatg aatctcttat aataagtatt tttttatcag
    23161 ataatgcatc ttcatttcta atttttaaaa tagaacaagc attgccaaac atatatgcac
    23221 caccatataa aatttctttt tcatttttac gagtagctat aacatcttct tccttgcatt
    23281 tagtttctgt ttttccatca aatttatagt attcataatt aggcttctct tttagatgaa
    23341 cgtatggaat agtctcatct tctttaacta gtttatttaa atttttatta taacttccta
    23401 gaaaatatcc ttcatcatac attgtttgag tataactatc ccaagaaata ttatttacta
    23461 tattcatatc ttcaattata catttaaagc cttcataagc tcctattcca ttccagtgat
    23521 ggtctgtttt aaaatacaag tcttctcgtt gagattcatt aaactctttt aaaaaatgtt
    23581 catctacatc tataacgttt atagtttcta aatccaaata acttttgaaa gcatttttat
    23641 tactaatatc attttttagc ccatcaacat atttggggta taaatgtgct agcatacttt
    23701 ctttatttgg agtagaaaca taataaactt ttttatttga atcagatgct attttagata
    23761 attcatttat ttcatttgca gcatctttta attctttttc agacattgat tttgatggtg
    23821 ttggcataat ccagttatca tctagtaaat aatagttttt aattttattt ttaccaagga
    23881 ctatttgtac cttagagtat atttcagata actcttctct aaatggaaac tggtctgaaa
    23941 aataactttc aaattcgctt gtatattttt caaatttaaa atcttgtata gttggttttt
    24001 gagccaacgt tctattttca gatgcactaa tttctttatc tttacttact atgtgtataa
    24061 ggaatactcc tattattata actacaaaag gaatcgatat ccactttgat ttcttattat
    24121 tcattttaat ttgccccctt agaatctgaa atataaaaat ggattatatg tagaatttat
    24181 caatattaca actactataa acattagtgc cactaaaaaa gttgagtgga gcatgtatac
    24241 aaaattactt tcaaccagtt ttttagtttt tgattttaat attgacttaa gcttaggtat
    24301 tataggtatt gcaaatataa tagataaaat tataatatat ccataatcat ttatatacat
    24361 aataaaggag ttattacata ttggacttga gccaactcca aataaaactt gtacaaattc
    24421 agatgcttgc actatattat ctgctctaaa gaatacccat ccaatcatta ctattagtat
    24481 taagtatata tgtctaaata tctgtggtac cttttttagt aatttaccta aaaagacttt
    24541 ttctatacta attaagattc cataatatat accccatgta agaaatgtcc agttagcacc
    24601 gtgccatatt cctgttaaag accataccac aaataaattt gtatactgct taataggaga
    24661 aactctgttt ccacctaaag gtatgtatac atagtctcta aaccaactac ttagcgatat
    24721 atgccatctt ctccaaaatt cagatacaga ttgtgatatg tatggatagt tgaagttttc
    24781 aagaaaatca aatccaaata ttttaccaag tcctatagcc atatcactgt aacctgaaaa
    24841 gtcaaagtat atttgaagtg tatagcatat tattcccagc cacgattcag ctattgaaag
    24901 attcgctata tttgctgaaa aaactccatc tgctaccatt ccaagttgat ttgctaaaat
    24961 aactttctta gctagtccac atataaatct atttactcct actgcaaatt tttcattaga
    25021 ataaatcctc ttacttattt gctcatctac agtttgatac ctaacgatag gacctgctac
    25081 aagttgcgga aataaactta catatagtgc tagatcaaat atatttcttt gaactcttcc
    25141 gtcttttctg tatacatcaa ttacatagct cattgtttgg aatgtgaaaa atgatatacc
    25201 taagggtagt gctattgttg ggatgcttat atgagaatta aataaagcgt taaaattgtc
    25261 tacaaagaaa ttagaatatt taaaaattac caataatgat atgttaaaaa tcacagataa
    25321 tgttaaccat aatttctgtt ctttcttatt gtctgaagat actttaagtc caaatatata
    25381 attgactact atagacccaa gcattaagaa tatatattta ggttctcccc atgcataaaa
    25441 gaatagactc gtaagtaata acacaaaatt tttaagttta tcattaacta aaaaatagag
    25501 tataaaaact acaggtaaaa acgcaaataa aaatgttaag ctactaaaaa tcatattttt
    25561 atatcaattt aaatcctcta aattgatata ttcacctcct ttaatcaacc catttatgga
    25621 tctattttat tgtataacta catttgaaca aactttgttt taaaaaaatg tataatagtg
    25681 gactaacata gttatattta gacaaaatgt attagtatgt aaatttaagt aagagtagga
    25741 cttaatttta taattagaac catctcttga taatcataaa tgttattttt gacagactta
    25801 tgccctatat aaatataaaa tgatataaac gcataaaatc acaatatttt tttgaatcta
    25861 agtctattct atattactta tataggttaa cataataaca taaaaaacat attttgcaaa
    25921 tatttttaat ttaattgtaa ttatttgtta ttgaaaatct gctttatata tactcatctc
    25981 gacacaaaat attataataa caagtagcac ctgtaaatct ccaaacatat tgcaaatact
    26041 tattcacata tatttttcag ttctaattat aaattcatta tatttttagt tgtgtaaata
    26101 aattttctat aaaaataaat gcattgtatt attttttgcc taaaaagggg atgactcaaa
    26161 attttatttt gagccatccc catataaata attataaata tttaagcaat cttgttgttc
    26221 aaattcttat tttctctttt gtttatctta tttaaagcat aagtagcaaa tatccctata
    26281 actgcacata ctacagataa aataaatata tatgtatatc ctgtttgtcc atatttatct
    26341 aaccaattac ctgcaatagt gtaataaaat gcgtctggag aaaatcctat aaatgaagca
    26401 aatccaacta ctgctcctgt agtttctaaa gatatattag actctgaaac agatgcaaag
    26461 tatatacctc taaatccaaa taaaattaca cttagtatta tcatgtttat tacagctatc
    26521 catataaatc ctgtactctc aggaatcacc aagtaagcag ctgtacttac tgtcattagg
    26581 ataaatccta caaacattac tcttatagat gaaccaactt tatctgttat aacaccagca
    26641 actggagatg ctcccatttt tataacataa gttcttatta ttgataaagc agaaactaga
    26701 gtcattggca ttacataaac ttctgataag tatggactta aataagttaa acttgagaat
    26761 actaaataag ttgaaaatat tataagacca attagccagg cttttggcat tgtcactact
    26821 tttagtaatg acttaatact taatccttca tctgatgatc cactatcttt tttctcttct
    26881 actaagaaga atagaagtat accacatatt atagatgctg cagaatataa ccatactaca
    26941 tatttgaatc ctactgttat ttcagcaaat ttaccaaaga aataaagacc tataaaagaa
    27001 attaatgtac ctgatacacc tgataaccct tcataaaaac caaatattct tccttgctct
    27061 gactcacttc cttgcattct aacaacctta acagatgctg agaagaaggt taatattgtc
    27121 gtaaatcccc ataatacaaa tattaaaagt atcatactat atgacgggaa tgtagagaaa
    27181 taaaatccaa gtattcctga agcaattaaa gaaaaagtta ttagttttct aactgagaat
    27241 ctatctgcaa accatcctcc tataaaataa gataccattc ctactaatcc atataaactt
    27301 aataagttcc ctatttgtgt atgacttaat gctaaagctt gttgcattgg gtcataaaat
    27361 gatgatttca tatatggtaa actatacata gctgttgttc caaatgccaa tatgaatatt
    27421 atcaaaaatc ttttaacatt atttttgctt tccattgatt ctctctcctt aaaattttat
    27481 tgtttataat ctcattcatg taattttatt ttaatttttt tattgcttgt ttaaagtcat
    27541 ttattatatc atttgaagat tccaatccta cagctattct tattagattt tctccattca
    27601 tttgaactaa tgtatgctct tcccctaagc ttgttgctat tgttgcaagc tctaaagagt
    27661 tcattaatct tctactagct tggtctcttg tcaatccatt gataccatcc ttaagtttaa
    27721 atgaaactac acctccacct aaaccattca tttgagtttt acaaagctca tattgaggat
    27781 gagattttaa tgctggatac tttaccaatt ccacattctc ttcttcctct aaaaactctg
    27841 ctactgctaa tccattttca caatgctttt tcatcctcat atctaacgtt ttcatacctc
    27901 ttaaaatcat ccatgcactc attggtggta aagatgctcc tgtaaaacaa cataaacttg
    27961 gccatctaac taaatctatt aattcttttg aacctgctac tgctccccct aaagtatccc
    28021 catgtccatt tataaactta gtaagactat gtattactaa atctgcacct aaatctattg
    28081 gtctttgcac aattggtgta ccaaaagtac tatcaactat ggctaaactt ttattttcat
    28141 gtgcaatttc tgctattgtt tttatatcta ctaagtctaa tgatggattt aaaggacttt
    28201 ctatataaac tatcttagta tttggtttca ttgcatcttt tacattttgt ggatttactg
    28261 catctacaaa tgtaacttca actccaaatt tagataacaa ctcagacatg aacaatttag
    28321 tgtgtgagaa tattccatct gaactaacta tatggtcacc actttttacc aatgctaata
    28381 tagtagatgt tattgccccc atccctgatg tactagatat ggcactttct gcattttcaa
    28441 gcatagccaa cttagcctct aactctgtaa gagtaggatt acactctctt gtatacacat
    28501 atccccatgt catacacaac tcttcaaaat gctctactgt ctttgcagca aatgttgctg
    28561 tttgacatat tggtgtagcc aatgcacctg ttgttgggtc tgcactatgt ccccaatgta
    28621 ttatttttgt ctctaaatct tgattaatat tgttactcat tttaatttcc tcccattttg
    28681 acttatttat acaacgtttt ccctgccaaa tttcatcaat ttagcttagt attaatacat
    28741 tttttcaagt atactttttt atttgttata atttctaaaa atatgacact tattttgtct
    28801 ttttacagtt ctaattataa actctatagt aactatagag tcaatatgtt tttgatattt
    28861 tttttacaat atttaaaat
//
LOCUS       DAEMAE010000022        28853 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEMAE010000022 DAEMAE010000000
VERSION     DAEMAE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.022"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1054)
                     /locus_tag="KPH93_000826"
     CDS             complement(<1..1054)
                     /locus_tag="KPH93_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687661.1"
                     /translation="MIRLTVANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSENQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDNLQRPVVKCFKIENGGGSLWAESQIDSFITKSLKLKMDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWIARVFKNESSIS
                     LISTFYSLIGLAISLSSLIPSMDKLSFMDNLSKLTPFYWTIDAIKNNGSILINIIILI
                     LIGIIFVTAGSLKLRDF"
     gene            complement(1054..2154)
                     /locus_tag="KPH93_000827"
     CDS             complement(1054..2154)
                     /locus_tag="KPH93_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687662.1"
                     /translation="MKLYMSVKTMLKGLKSAFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAVLLQDILDTYHEKFYLNNSQKISSEDFYKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTVIVYALLFLQTIFGGIFSMISDVFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLITSTILIALAIVKEKYKWREVQ"
     gene            complement(2168..3103)
                     /locus_tag="KPH93_000828"
     CDS             complement(2168..3103)
                     /locus_tag="KPH93_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687663.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERINEALEVTGLTEIKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLILEIKNINAQLIFDLKNTKGVISVLENNSLLELDIAKKLQLTDI
                     LSIIDKNDSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(3232..3879)
                     /locus_tag="KPH93_000829"
     CDS             complement(3232..3879)
                     /locus_tag="KPH93_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687664.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDKVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEYLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(3872..5035)
                     /locus_tag="KPH93_000830"
     CDS             complement(3872..5035)
                     /locus_tag="KPH93_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1687665.1"
                     /translation="MNRKKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDTSFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNPSSFIITISD
                     NGIGCGNIKKGNGLNSISERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            5219..5563
                     /locus_tag="KPH93_000831"
     CDS             5219..5563
                     /locus_tag="KPH93_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG1687666.1"
                     /translation="MNNKEKGDFGEKIAVNYLLSKGAKILEKNYRLKIGEIDIIAKMG
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            5594..7195
                     /locus_tag="KPH93_000832"
     CDS             5594..7195
                     /locus_tag="KPH93_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG1687667.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPSKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSVLNDKSRKENEELEKDNSYINCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            7214..8314
                     /gene="dprA"
                     /locus_tag="KPH93_000833"
     CDS             7214..8314
                     /gene="dprA"
                     /locus_tag="KPH93_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG1687668.1"
                     /translation="MEKRDTYLWLKSISGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDISLVNNNFNIAIVGSRKPTTYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLNLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            8347..10434
                     /gene="topA"
                     /locus_tag="KPH93_000834"
     CDS             8347..10434
                     /gene="topA"
                     /locus_tag="KPH93_000834"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG1687669.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVDNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPDSIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYEPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            10599..11384
                     /gene="codY"
                     /locus_tag="KPH93_000835"
     CDS             10599..11384
                     /gene="codY"
                     /locus_tag="KPH93_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG1687670.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDAQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            11491..12756
                     /locus_tag="KPH93_000836"
     CDS             11491..12756
                     /locus_tag="KPH93_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG1687671.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDIIRSSTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            12787..13557
                     /locus_tag="KPH93_000837"
     CDS             12787..13557
                     /locus_tag="KPH93_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1687672.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            13666..14112
                     /locus_tag="KPH93_000838"
     CDS             13666..14112
                     /locus_tag="KPH93_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG1687673.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            14130..15323
                     /gene="nifS"
                     /locus_tag="KPH93_000839"
     CDS             14130..15323
                     /gene="nifS"
                     /locus_tag="KPH93_000839"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG1687674.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     EGLVK"
     gene            15377..15817
                     /gene="nifU"
                     /locus_tag="KPH93_000840"
     CDS             15377..15817
                     /gene="nifU"
                     /locus_tag="KPH93_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG1687675.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGRTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            16110..17189
                     /gene="mnmA"
                     /locus_tag="KPH93_000841"
     CDS             16110..17189
                     /gene="mnmA"
                     /locus_tag="KPH93_000841"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG1687676.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDDVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    17331..17569
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17637..20276
                     /gene="alaS"
                     /locus_tag="KPH93_000842"
     CDS             17637..20276
                     /gene="alaS"
                     /locus_tag="KPH93_000842"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG1687677.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAKEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDGETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            20301..20558
                     /locus_tag="KPH93_000843"
     CDS             20301..20558
                     /locus_tag="KPH93_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG1687678.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            20568..22739
                     /locus_tag="KPH93_000844"
     CDS             20568..22739
                     /locus_tag="KPH93_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687679.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVRNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            22945..23370
                     /gene="ruvX"
                     /locus_tag="KPH93_000845"
     CDS             22945..23370
                     /gene="ruvX"
                     /locus_tag="KPH93_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG1687680.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            23448..23735
                     /locus_tag="KPH93_000846"
     CDS             23448..23735
                     /locus_tag="KPH93_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG1687681.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            24017..24475
                     /locus_tag="KPH93_000847"
     CDS             24017..24475
                     /locus_tag="KPH93_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG1687682.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(24592..25329)
                     /locus_tag="KPH93_000848"
     CDS             complement(24592..25329)
                     /locus_tag="KPH93_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG1687683.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            25546..27222
                     /locus_tag="KPH93_000849"
     CDS             25546..27222
                     /locus_tag="KPH93_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG1687684.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            27430..27630
                     /locus_tag="KPH93_000850"
     CDS             27430..27630
                     /locus_tag="KPH93_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG1687685.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            27684..28847
                     /locus_tag="KPH93_000851"
     CDS             27684..28847
                     /locus_tag="KPH93_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1687686.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
BASE COUNT        11583 a         3133 c         5002 g         9135 t
ORIGIN      
        1 caaaatctcg taatttaaga cttcctgcag ttacaaatat aattccaatc aatatcagaa
       61 ttattatatt tattaagata ctaccattgt ttttaatagc atctatagtc caataaaatg
      121 gtgtaagttt tgaaagatta tccataaatg ataatttatc cattgatggt attagactag
      181 atagagatat agccaaacct attaaagaat aaaatgtaga tataagactt atagaacttt
      241 catttttaaa tactctagct atccaaaata caaatccagt ggaaacaaaa ctatttgcta
      301 gtacaatcat caatacattc agactcaaat taaagtcttc tataattgaa aaacataata
      361 gagcaaaagt gtatacaatt gattgaataa aaaataaccc taaaaatata ctgaaaatta
      421 tttttatatc tttattatct gtagagataa gtctttttag cacatttgat ttttttaatg
      481 taaataaatc agaagcaaga tgtgccgcat taatatacat aaaatagcat atcaagaaga
      541 ctactaaaaa tgaaccttta ttcttatctg gtttgttatc aattatattt gtctttgtca
      601 atttatcatc tatattttta tccattttca attttaacga tttagtaata aaagagtcta
      661 tttgtgattc tgcccaaaga ctaccaccac cattttcaat tttaaagcat ttaactacag
      721 gtcgttgtaa gttatctata ctatttgaaa aatttttatc taagacgaat acagcagaaa
      781 ccttattatt ttttaataga ttcatagcct tatcttgttc atttaatcta aatgaatttt
      841 cttttaactt agaactattt attaacttat gttcatactt tcctgaagag tcagatacaa
      901 ttgctactgg agaaaagtac attgattgat tttccgagtt gttgataaaa aaattaagtg
      961 aaaaaattag tataatagga agcataacca tattaattaa aagagtatga ttttttagat
     1021 atctttttat atttgcaaca gttaatctaa tcattattga acctccctcc atttatattt
     1081 ttcctttaca atagcaagtg caatcaatat tgtagaagta attaaacatg tgaaaattaa
     1141 atttcctatt gaatctatag ttttaaatgt ttcataatta ctaaacaatt ctcctattaa
     1201 ataggtagga gataaacttg taagcttagt aaatacatca cttatcatac taaagatacc
     1261 tccaaaaatt gtctgtaaaa agagtaatgc ataaactatt actgtaccat attttttact
     1321 aaaaaagctg gttacaaagg tagatattga agtcataaaa taagatgata aagcacaaag
     1381 taggagcaat atggcaaaat tacctttaaa tgttattcca agtattctaa agaataaaac
     1441 atataataat aagaatataa atgaataaat ccataaacct acaaagtcat aaattaagaa
     1501 ttgtactcta gttataggca tagaataaag tctttttgaa agtccctttg attcacttat
     1561 ataatttgat aaaatattgt tcattataaa aattattaca agaaatccta gcgaaactaa
     1621 agcaaagtat tcataagagc tctgtttaac attgttattt attatagaag tatcaataga
     1681 atttttatta aataatttat agaaatcttc tgaacttatt ttctgggaat tatttaaata
     1741 aaatttttca tggtaagtat ctaatatgtc ttgaagcaat actgcaatat catcacgagt
     1801 tccaagtttt tctatattta gagtatttga tttttcattt aataaactac tctcatagcc
     1861 tttaggtata gtaagcttta attctgcctt agaatcatct tttttaactg ttaatatata
     1921 agataaatca tttttcaaat actttgtcaa atcatttgat aatcttgtgt tgtctttgtc
     1981 atatataaca atgggtgtag attctgtttt tatagggttt tgaaacatgc tttctgtaac
     2041 cataccaaga aaccaagata aaatcagagg taaagctaaa aaatatatta aattaagtat
     2101 aaaggcgctt tttaaaccct ttagcatagt ttttacactc atgtataatt tcataaaatc
     2161 acctccatta gtcccttaaa tttttgcctg ttagatttaa aaatacatct tctagactag
     2221 gttcttcata agaaattttc attatttttg aatcgttttt gtcaattata gagagtatat
     2281 ctgtaagttg taatttttta gctatatcca attctaataa tgaattattt tctaaaacag
     2341 atataacacc tttagtattt tttaaatcaa agataagttg agcatttata ttttttattt
     2401 ccagaattaa ttttgtattt gtagatacta gtgattttaa ataatcatta tctccaaaag
     2461 caatttcttt accttcatcc ataataaaaa ttttagaaca aaggtgttca acttcttcca
     2521 tataatggga agtatatatt acagtagttt catgtttttt agatatatct tttataaaat
     2581 tgaaaatatg atttcttgat tgggggtcta cacctacagt tggttcatcc aatattaata
     2641 ttttaggatg atttaagagt gctattccaa tatttaatct cctttgcata ccaccagata
     2701 attttttgac ctttttcttt ttgatttcag taagacctgt aacttctaat gcttcattga
     2761 ttctttcttt aagtagtttt ccttttaaac catagaaagc tccaaagtac tctaagttat
     2821 caaaaggagt aagagtttct ataattgcta attcttgtgg aactaatcct attatagact
     2881 ttgcttttat ataatctttt gataaatcca aaccattgat ttgaatacta ccactatttg
     2941 gtaacataag atttgtaatt atatttataa gtgtagattt acctgcacca tttggaccaa
     3001 taagtccaaa tatctcgcct tttttaacag aaaagctgat gttatcaagt gctaatttat
     3061 cattaaatct ttttgttata ttatttactc tgactatatc cataataatc cctccaaaat
     3121 aattttatat aagttaaaaa gctataatac tataataaag aattgtgaat tataattcta
     3181 ttactataag tcactaaaaa aagtgactta agtcacttta tatttttaga tttattctat
     3241 tacaatttta tttttgaaag caaagattgc tagttgagtt ctgtcacgaa gttctagttt
     3301 agctaaaata ttagttatgt tattctttat tgtaccttca gataagaata gttcttgact
     3361 tatttcttta ttattaagtc catttgcaat tagtctaatt atagatattt ctttttctgt
     3421 taaattgtac atgtcataaa gatactcttg tttaatcgtt ttttcagagg tcatgatttt
     3481 ttcagctaca cttttatcaa gcacaatatt gccaaaaaat gaagagcgaa tcccttcatg
     3541 caatacttta tcagagctat cttttaaaag gtatcctgaa gcaccaaact tcatagcatc
     3601 ttttatatat tcatcatcat taaaagttgt taaaattaat aatgttatag aattattata
     3661 ttcctttata agccttgtac ctagaacccc atcacatact ttcattcgta tatccataag
     3721 aactatgtct acatcattcg ttttacaaaa ttctaaagct tcatatccat tagaagcatc
     3781 tcctacaatt tctaaatcat tataagatga taatataatt ttaagacctt ttctaattaa
     3841 tttttcatca tctactataa gaactttaat tttattcaaa aaaatcaccc cctgaaaatt
     3901 taggaaaaga agctctaatt gtgaaaccat tattagaatt atcaaattca actttaccat
     3961 ttaattcata tattctttca ctgatactat taagtccatt tccttttttt atattgccac
     4021 atcctatacc attatcacta atagtaatta taaaacttga agggttgaat gtaatgaaaa
     4081 ttctgatttc agttgccttt ccatgcctag atgagttgga aagggattcc tgtataagtc
     4141 tatataaagc tatagattga tttctagata agttccaagt attttttgaa atagttaatt
     4201 taacattaat atttgtaagc ttaataaagc ttttaactaa ttctttgatt ttaaataaac
     4261 tttggtaatt ttcatattca acaggtttta actcagatat agcccttctt acatcttgaa
     4321 aactttcggt tacaaattct ctaagttcat gtactaagtc ataaattaga ggtttgtctt
     4381 ttaagagttt ttctatagca tttaattgta ttattgttgt agaaagagaa tgtcctacac
     4441 tatcatgtat ttctcttgat attcgatttc tttcttttaa aattgctaat tcttctatag
     4501 aacttgcata agtttccaaa tcagcattaa ctttttttaa ttcatcttta gaaattctaa
     4561 gcttatcata aagattttga ctgtataatt ttgataaatt ctcattttct atatatatag
     4621 aaagtacagt tataattagc aatatagaac ttgactccag tgccaaatat atatttttat
     4681 ctaaactaat aataagagaa gatattataa caaaagagaa caatatatac ttatattttt
     4741 cctttattaa aaatgatgta tctaatatta aaggaacaaa gtaaatcgaa ttaaaagttt
     4801 ttgtataatt acaaagtata aaaattaata taagttctaa aaagtaagaa ataagtataa
     4861 atttattctc atttgataaa gaaaaaaatc taacttgatt atttattata attagaaata
     4921 atattatcat agttatgacc ggatacaatg tgttttcaac atttttcaaa tagcttacaa
     4981 ctaatataaa taaagataaa tatcgtataa aagcaactaa tttttttctg ttcattaaag
     5041 gcactcctcc aaaaaaataa acttttccta tgaaaactag aataatatca taaaagaata
     5101 aagtcaatag aagtatttat taatattgat ggaaataaaa tcaattcaaa aaattgaata
     5161 gtaaaattgt atactgacca taatttaaaa gtgaaaattt aatgtattgg agtgaataat
     5221 gaataataaa gaaaaaggtg attttggaga aaaaatagca gttaattatt tgctatcaaa
     5281 aggagcaaag atactagaaa aaaattacag attaaagata ggggaaatag atataatagc
     5341 aaaaatggga gatgaaatta tatttgtgga ggtgaaatca aggtctaaca taaagtttgg
     5401 ctacccttgt gaatctgtaa gcttcaaaaa aagaaaaaag ataattggag ttgcaaatta
     5461 ttatattata aaaaataact taaataacac ccccataaga tttgatgtta tagaggtata
     5521 tttattagaa aaaagaataa atcacataat gaatgctttt taaaaaagat aatgtttttg
     5581 gaaaggatta aatatgttaa gtattattaa ttcaagcaac ttagtaggaa tcgacagttt
     5641 tttagttaag gttgaagtag atgtaagcaa tggaatacct agttttaata ttgttggtct
     5701 tccaagcaaa gaaattaaag aagcaagaga aagagtaaag tcagcaattc ttaatagtgg
     5761 atataaattt ccaagcacga gaatagttgt aaatttatca ccagcagata taaaaaaaga
     5821 aggagctttt ttagatttat ctataagtat aggtctttta agagagttaa taaaaaaaga
     5881 tgaaaattac ataagagaaa gtatgtttat aggagaatta tcattagatg gaaaaataag
     5941 aaaagtaaga ggtatattgc ccataattat gggtgcaaaa actcagaaca taaaacgtat
     6001 ttttatacca attgagaaca taaaagaaag tctatttgta gatgaaattg atataattcc
     6061 tattaaaagt ttaaaagaat gtgtcgattt tttaaacgaa gaaataaaag tcgataaagt
     6121 aaggataatg tctgttttga atgataagtc aagaaaagag aacgaagagt tagaaaaaga
     6181 taatagttat ataaattgca aatatactaa gattaataat gaagaaagta aatatgatga
     6241 agactttaaa gatgtaaaag gaaattattt tgtaaagagg agtgcagaaa ttgcagcagc
     6301 tggaaatcat aatatgttta tgattggacc tccaggctca ggaaaaacta tgattgcaaa
     6361 aagagtaagg acaattttac cagatataag tatagaagaa atgatagaag taagtaaggt
     6421 ttatagtata ttgggtatga taaatgaaac taaaggaata attgataaaa gaccttttag
     6481 agcacctcat catacaacta caaagcaatc tttaattgga ggaggaatgg atgcaagacc
     6541 tggagaaatt gctttagcac atagaggtat cttattttta gatgagatag ccgaatttga
     6601 tagaaaaata cttgagactt taagacaacc tatagaagat ggatatgtaa atatatccag
     6661 agtaaagtac agtgcaaaat atccttgtag agtattgtta gttgcagcta tgaatccatg
     6721 tccatgtggt tattatatgt cagagacaga gtgtagatgt agaagtaacg aaattgatag
     6781 atacataaat aaaatatcag gtccattgtt agataggttt gatatttttg tagaagttaa
     6841 ttctataaaa tattcagatt ttaattcttt aaaacaagaa gaaagctctc aaaaaataaa
     6901 gagaagagta gaaaatgcta gaaaaataca gataaataga tttaagaagg ataatataaa
     6961 aaataatagt gaaattaaag cttacaattt atttaaatat tgtaaactgg aaaaagaagc
     7021 ttcaaaaacg gctgagatga tatttaataa atacaactta agcagtagaa gttatacaaa
     7081 acttttaaaa atggcaagaa caatagcgga tttagaagaa agagacttga taaattcaca
     7141 gtgtattata gaagcttttt catttagaaa ggcttattat agttatttta aatagttttt
     7201 tgaggtggat tttatggaaa aaagagatac ttatttatgg ttaaaatcaa ttagtggaat
     7261 aacaacaaaa acaattgaaa taatagaaaa tgaaatagta aatattgaag atatatttga
     7321 tttttctgaa aaagaaatat ataatttaaa aaatataagc ttgaatatta gaaaaaatat
     7381 agtaaaatat agaggtcacg cttatttaga aaatataaaa gaactacttt acaaaaaagc
     7441 gattaaatat atttgtaaat atgataaaga atatcctgaa aatttaaaga atatatacaa
     7501 cgctccaaag ttattattct acaaaggtga cataagttta gttaataata attttaatat
     7561 agctatagta ggttcaagaa aacctacaac ttatggaatt aattgtgcaa aaacaataag
     7621 ttgtcaacta tcccaatatg gagttaatat agtaagtgga ttagctatag gaatagatgc
     7681 atactcgcac ataggttgta tgagtggaaa atctaaaact atagcagtac ttggttcagg
     7741 agtagacaat cctcttccaa aacaaaattt aaatttatca aataaaatat tagaaaatgg
     7801 gggattatta ttatcagagt ataatattaa ctcgacagta gccccatacc atttttctaa
     7861 tagaaatagg attataagtg gtttaagtga tggagtagtt gttgtagaag cagcaataaa
     7921 aagtggtgca ctaatcacag tagattttgc tcttgaacat ggaaaaaatg tttttgcaat
     7981 acctggtaat ataaattctc aaatgagtag aggttgtcat aaaattataa aagaaggtgc
     8041 aaaattaatt gaaaatatag atgatatttt aaatgaatat aacatattta atattattga
     8101 taaaaaaata aatcaaaaat atgataatat aagtctaaat gccaagtcta agcaaataat
     8161 tgaagctata aaaagagaag gaaacttgca tatagatagt atttgtgatt atactggtat
     8221 agaaataaag tatgtaaatt caataatcaa tgaattagta ctaaatgagt tagtagtaga
     8281 aatgaataat aaaacgtaca gtttaaatgt ataaattaag attaggtagt gggggtgtat
     8341 ggttttatgg ccaaaacatt agtcatcgta gaatcgcctg caaaagctaa aactatagaa
     8401 aaatttttag ggaaaagtca ttacacagtt aaagcatcag ttggtcatgt cagagattta
     8461 cctaaaagta aattaggtgt agatatagaa aataattttg aacctcaata tataaatata
     8521 agaggaaaag gcgatgtaat aaaagaatta aaaaaagaag ctaaaaaatc taaacaagtg
     8581 tatctagcaa ctgaccctga tagagaaggt gaagctatat catggcattt agctcatata
     8641 ctaaatttag atgaatctga agattgtaga atagagttta atgagataac taaggatgca
     8701 attaaaaaag caattaaaca tcctagaaat attgatataa atttggttga tgcacaacag
     8761 gcaagaagag tgctagacag acttttaggt tatcaaataa gtcctatatt atggcaaaag
     8821 gttagaaaag gtcttagtgc aggtagagtg caatctgtta caacaaagtt aatttgtgac
     8881 agagaaaaag aaataaaggc atttattcca aaagaatatt ggacaataga tgttgaggct
     8941 aagacagtaa aagacaaaga tataactttg aaattttatg gtaaaaatga tgaaaagatt
     9001 gaattagaaa atgaagaagc tgtaaatgaa atactaaaag aaatggaagg taaagattta
     9061 aaggttgaga agatagaatc taaatcaaga cgtaaatcag ctccaaaacc atttacaaca
     9121 agtatgcttc aacaagaagg agcaaataag ttatttttta ctactaaaaa aacaatgatg
     9181 atagcacaag aattatatga aggtatcgac atagaaggtg aaggtactgt tggtcttata
     9241 tcatatataa gaactgactc taaaagaata tctgatgaag caaaagaaaa agctaagggt
     9301 tatatcgtag acaatttagg agaaaactac tataaaaaca ctggtgataa aaaagaaaaa
     9361 aaggatacta aaaaggttca agatgcccat gaggcaataa gaccaacctc agttgataga
     9421 actccagata gtataaaagc ctcattgagc aaagaccagt acaagttata caatttaatt
     9481 tggagaagat ttgtagctag tcaaatggaa gattcagtgt ttgatatact aaatgtagaa
     9541 tgtaaaatag gcgatattgt atttaaggca actggttcaa agatgaaatt tgatggttat
     9601 accaaggtat ataatttttc agaaagagaa gacaaaattt taccaagtat agaaaaagga
     9661 gatatattaa atatagaaaa tatattgcca gaccaacatt ttacacaacc accagcaaga
     9721 tatacagaag ctagtttagt aaaaacacta gaagaactag gtataggtag accaagtacg
     9781 tatgctccaa caatagctac tatactgaat agagagtatg ttgaaaagca aggaacaagt
     9841 ctatgtccaa cagaacttgg aataatagtt actgatattt tggaaaataa tttccataaa
     9901 tttatagatg tggattttac tgctgatatg gaaagcaaac ttgatgttat agaagaaggt
     9961 aatacagatt ggaaaaaaat tgtatctgaa tcatatgagc cgcttaaaga ggctattgaa
    10021 gaagcacttg aaaatataga aaaagtaaat atggatgaag aaactgatga gatttgcgag
    10081 aactgtggtt ctaatatggt tatcaaatat ggtagatttg gaaagtttat ggcttgtaaa
    10141 aactatcccg attgtaaaac aactagacca attatgaata aaattggtgt taaatgtcca
    10201 aaatgtgaag aaggagatat tatacttaga aaatctaaaa gaggtaaagc tttttatggg
    10261 tgttctaatt atccaaactg tgactttgta gcttggaata agccaacagg agagttttgt
    10321 gagaagtgtg gttcatatat ggttgaaaaa gtgactaaat ctgaaacaaa aacagtgtgt
    10381 tctaataaag aatgtaataa agagacaaaa gagcataatg acggaaaaga gtgaaaattg
    10441 aaaaaaatca cataattctc ttgaaaaagg ttccaatcta tgataatctt tcaagtgtaa
    10501 acaactaata gaaaaataaa ttttcattaa atgtgaaaat aatattagaa aatataaaag
    10561 gttattaaaa ggcctaaatt tcatgaggag atgattaaat ggcaagtgaa gtgttacaaa
    10621 aaacaaggaa aataaataaa acattacaaa caagtggtgg aagcagtgtc tcttttgatt
    10681 tactggccgg agcattgggc gacgttttaa gttctaatgt ttatgtagta agtgcaaaag
    10741 gtaaagtact aggtcttcat ttaaatgatg ctcaagacag ttcagttata gaagatgagt
    10801 atactaagca aaagaaattt tcagatgaat atactcaaaa tgtgttaaaa atcgatgaaa
    10861 cattagaaaa tttaaatggt gagaagatat tagaaatctt tcctgaagaa catggaagat
    10921 tacaaaaata tactacagta gttccaatat taggaagcgg tcaaagatta ggaacattag
    10981 tactttcaag atattcaaat tcattcaatg atgatgattt agtaatagct gaatacagtg
    11041 caactgttgt tggtcttgaa atattaagag caataggtga agaattagaa gaagaaatga
    11101 gaaagaaagc tgtagttcaa atggcaatag gcactctgtc ctactccgag cttgaagcag
    11161 ttgaacatat ttttgctgaa ttggatggaa aagaaggtct acttgtagca agtaagatag
    11221 ctgatagagt tggtataact aggtctgtaa tagtaaatgc acttagaaaa tttgagagtg
    11281 caggtgtgat agaatcaaga tcattaggta tgaaaggtac tcatataaga atacttaatg
    11341 acaaacttac agatgaatta aaaaaattaa aaaacaatca ataatctata ttttataggt
    11401 ttagattaga aaaggtatct atttcaaata gatacctttt ttagacgaat tattaagata
    11461 atgataagta tattaaaaag gagtgagaaa atgcctttag cagataaaat aagaccaaaa
    11521 acaatgaatg atgtaatagg tcaaagtcat ataataggta atggaaaaat attgtctaag
    11581 atattacaat ctaatttttt accaaatatg atattttttg gtcctcctgg tgttggtaaa
    11641 actacagtag cggaaataat agcagaaaga agcaataaaa gcttctataa aataaatgct
    11701 actaattcat ctttagaaga tataaaaaag gtgatatcag agcttggaag tataaataat
    11761 gtaaatggag tcctattata tatagatgaa atacaaagtt ttaataaaaa acagcaacaa
    11821 tcaatattag aatttatgga aaatgggagt ataactttga ttgctagcac tacagaaaat
    11881 ccatatcatt atgtatataa agctctttta agtaggtcta ctgtatttga atttaaacct
    11941 ttagaaaaac ttgatataga aaaaggactg aaaagagctg ttgaagtatt aaatgaagat
    12001 agctatatgg atattgagtg taataatgat gctattgaag atatagcaat tctttctgat
    12061 ggagatatga gaagagcctt aaatattttg gaagtggtag tctactcaac taaaccaaat
    12121 aaggataata taacttatat agattcagat ataataaggt catctacatt taataaaatt
    12181 ataaactatg acaaaaatgg tgatagtcat tatgatattt taagtgcatt tcaaaaatct
    12241 ataagaggaa gtgacccaca ggcatctata cactatctag caagacttat aaaaggtggg
    12301 gatttgataa gtatatgtag gcgactttta gtaatagcta gtgaagatat aggtttagct
    12361 tatccaaatg ctattgtaat tgtaaaagca tgtgtagact cagctatgca acttggattt
    12421 ccagaagcga aaataccatt agcagaagct actattcttt tggctacatc tccaaaatct
    12481 aattctgcat gtatagcaat aatgaaagct atggatgaac tggataaaga gtttgttaag
    12541 gatataccaa atagcataaa ggatgctcac tattctggtt ctaaagaaat aggtaggggt
    12601 tgtggataca aatatcctca taattttaaa aatcattatg taaagcaaca gtatttacca
    12661 gatagcataa aagatagtat ttattacata ccacaagaaa ataaaactga gaaaaatata
    12721 aaaaaatatt tagaatatct aaatagtgat ttttagactg aactttatgt atcgggggta
    12781 gatattatga ttataaaact taactcaact tatcacagta gagttatgaa atacttaaaa
    12841 aaagaacctg agtacaattt gtttataata ggggatattg aaagatatgg atatggaaat
    12901 aattttttaa atatatgggc agatgtaggt gaacatggtg aaataaaggc aatattactt
    12961 aaatattttg aatttatgat gttttattct gatggagagt atgatgttga aggtttttat
    13021 aatctacttc gtaatactaa ttacgaagaa atatctggga aaatatgtgc tgtagatgct
    13081 ttagcaaaga gattaggact taataattta aaggtagttg atttttgcaa acttcaaact
    13141 aaaaagtttt tgatagataa taattgtaat gcaaaggtta agagaattag gcttggaaat
    13201 ctaaaaaaga cagtgaaact ttatgactta atagatgaat ttcacagtac aactttagag
    13261 aatttaaaaa atggactaag gacaggtaga ggatattgta tagagattaa caaacaggtg
    13321 gtgtcaatgg caaagagtac ttcagaaaat agaactcatg caatgataat tggagtagga
    13381 acacatccta aatatagagc taaaggactt gctacaaaat gcctaattaa actatgttca
    13441 gagttactga gagaaaacaa aattccttgt ttgttttatg ataatgaaga ggctggaaag
    13501 atatataaaa aactaggatt tgaaaacatt ggaaaatggg gaatttattc aaaataaaaa
    13561 attaaaatta atcttgacaa aattagtagg gtataataaa attatcttga aagtaattcc
    13621 tactaaaaca gtatgaatta gatttctata tagaggtgat aatatatgaa gttgtctact
    13681 aaaggtagat atggactaaa agcaatgttt gaattagcat taaatcaaga caatggacca
    13741 gtatcattaa aatttattgc taagaagcaa aaaatatcag atcagtactt agaacaaata
    13801 ttttcatctt taaaaaaatc gggtctagta aaaagtgtga gaggtgctca aggaggttat
    13861 ttgctttcaa agaatgctga agacataaca gtaggagata tacttgtagt attagaagga
    13921 ccagttgcat tatctgactg tgtactagat gaagatgtat gtgaaaactc aaacatgtgt
    13981 gtaacaaaaa tagtttggga aaagatgaaa aagggtatag aagatgttat agattcaatt
    14041 actcttaaag atatgataaa tgactataac aaaaataaat tagaaaatga tataacaaat
    14101 attaaaaaat agatttggag atgaatgtaa tggaaaaaag aagattgtat atggattatt
    14161 ctgcaacaac acctattaaa aaagaagtat tggatgcaat gatgccatac ctaacagatt
    14221 attttggtaa tgcatctagt tttcacacat tcggaagaga agcaaaagat gctttggata
    14281 aagcaagaga acaagtagca gcccttataa atgctgaacc tagtgaaata tattttacag
    14341 caggtggttc agaaagtgat aactggacac tagagggtgt agcttatgct aataaaaaca
    14401 aaggtaatca tataataact tcaaaaatag agcaccatgc aatacttcat acatgtgaat
    14461 acttagcaaa acatcatgga tttgaaataa cttatttaga tgttgatagc gaaggaaaag
    14521 tagacttgaa acaactagaa gattcaataa aagatactac gatacttata agcataatgt
    14581 ttgcaaataa tgaaatagga actatacagc ctattaaaga aatatccgaa atagctaaaa
    14641 aacataagat attattccac actgatgcag tacaagctac aggtaatatt ccagtagatg
    14701 ttaaagaatt aggaatagat ttaatgagta tgtcttcaca taaaatatat ggaccaaaag
    14761 gtgtaggagc tttatacata agaaaaggtg taagacttca taactttgtc catggtggag
    14821 cacaagaaaa aagcaaaaga gctggtacag aaaatatccc tgcaatagtt ggatatggaa
    14881 aagcagcaga attagctaag gaaaatatgc aaaatcatgt agaaacttta actcgtctta
    14941 gaaataagtt aatagatggt gtgttagaaa gaatacctta tacaagagta aatggtagct
    15001 tagaaaatag attaccagga aatgcaaact ttgcattcca atttatagaa ggtgaaggaa
    15061 tacttttatt attagatatg ttaggaattg ctggttcaag tggttctgca tgcacttctg
    15121 gttcattaga cccttcacat gtacttcttg caataggttt accacatgaa atagcacatg
    15181 gttcattaag acttacagtt ggagatttta caacagatga tgatatagat tatatactag
    15241 agaacttacc aaaagtaata gaaagactta gaagcatgtc accactttat gatgatgcaa
    15301 agaaagaagg tttagtaaaa tagacagaaa gataaagcat aaaaaataga cataaattga
    15361 gtaggaggaa aagataatgc aatatagtga taaagttatg gaacatttta tgaatccaag
    15421 aaatatggga gaaattgata atgcaagtgg tgtaggtgaa gttggaaacc ctacatgtgg
    15481 agatataatg aagatatttt tagacataga tggagatgta ataaaggatg ttaagtttaa
    15541 aacatttggc tgtggttcag ctatagcaag ttcttctatg gcaactgaaa tgataaaagg
    15601 aagaactata aaagatgcat tggagcttac aaataaagct gttgcagaag ctttggatgg
    15661 tttaccacca gtaaagatgc attgttcagt tttagctgaa caagctgtaa aagcagctct
    15721 tatagattat gctcaaaaga acaatatcca tataccagaa ttggatggat atgttataga
    15781 tgatgctcat gaccatgatg ttgaagaaga agaataaaaa taagtgattg tgtgtgttat
    15841 gagaagttta caatgattaa aaagaagtac taatgatata attaggacat aaaaaatagc
    15901 tgtttttaaa atggctattt tttattgcct aaattaagta ttttaacttg atttagaggt
    15961 taattagtta acaaaataat ccataagatt caaataataa agatgattat ttttttagaa
    16021 aatagaacaa gctatatttt aaataagtaa aatgcttgct ctatatggta taataataag
    16081 taatgcaaaa gatgaggtga aatttgttta tgaataaaaa agtaatgata ggtatgagtg
    16141 gaggagttga tagctctgta gctgcatatc ttttaaaaca acaaggatat gatgtaattg
    16201 gtgttactat gaagttgtgg caagatgatg acgatgttga aattgaaggt ggttgttgct
    16261 ctttatctgc tgttgaagat gctagaaggg tagcaaataa aataggtata ccattttatg
    16321 ttttaaattt tagagaagtg tttaaagaaa aagtaataga ttattttata gatgaatatt
    16381 tggaaggaaa aactcctaat ccctgtatag cttgtaataa acatataaaa tttgatgatt
    16441 tttataagaa ggctagacaa ataggttgtg attatgtagc tacaggtcac tatgcaaaga
    16501 tagaaaagga tgaaagtaca ggaagatatt tgctaaaaaa atctgttaca gataaaaaag
    16561 accaaactta tgcactttat aatcttacac aagagcaact agagcatact ttattaccaa
    16621 taggtgacta tgaaaaagat agagtaagag aaattgctaa ggaaatgggt atggcagtac
    16681 acaataagcc tgatagtcaa gaaatttgtt ttgtcaaaga taatgactat gcaaattatg
    16741 taaaaaaaca ttctaaaaag cgtattgaag aaggtttttt tgtagatact aaaggcaata
    16801 tacttggaaa acataagggt atattatatt atactatagg tcaaagaaaa gggcttggaa
    16861 ttacttttgg aaaaccaatg tttgtaatag acataaatcc aataaataat accatagttc
    16921 ttggagataa tgaagattta tttaaaaagg aacttattgc taaagatgtt aactttatat
    16981 caatagatac gttagaagaa ccattaagag ttcaagccaa aataagatat tcagcaaagc
    17041 catcaccagc tactattcat agagtaggag aagatactat aaaaatagtt tttgatgaag
    17101 ctcaaagagc tattactaaa ggtcaatctg tagtaatgta tgatggagat attgtagtag
    17161 gtggaggtat aatcgagaaa agtttataaa aacatctaaa aaattaaaaa aataaacaat
    17221 aaagtatatt aagagtaaat aaagtttata aaataaattt attgtatatg aaaatatagt
    17281 attgattaat tttgtaaaat ttagtaatat aattagtata ttattaaaaa acaaatacag
    17341 tgataaggaa tagtaaaata tttgatgtct tacagagaga gggaaatgct ggaagccctt
    17401 agaatatgat atttgaagca gccttttagt agcaattttg aacaatttat cttttataaa
    17461 gaagatttaa gtagagattg acggtagtta cgttatagct aaggttttcc gaagatatca
    17521 tactttgtat gataattagg gtggtaccgc gattgtcctc gcccctataa atttatttat
    17581 agggacgagg ttttttattt aaacgaaaaa ataaaattta ttaggaggaa catgatatgg
    17641 aaaagatggg attaaatgaa ataagaagta agtttttaga attttttgag tcaaaaggtc
    17701 attatgtagc aaatagttat tctttagtac caaataatga taagagtttg ttacttataa
    17761 actcaggtat ggcacctttg aaaaactact tttcaggtgt agaggtacct ccaagtgtga
    17821 gaatgtgtac atctcaaaaa tgtataagaa caggagatat agaaaatgta ggtataactg
    17881 caagacatgc tacattcttt gaaatgatgg gtaacttctc atttggagat tactttaaaa
    17941 gagagtcaat caaatgggga tgggaatttg ttacagaatg gctaaatata ccagaagata
    18001 agatatgggt gactgtatat gaagaagatg atgattctta tgatatatgg gcaaaagaaa
    18061 tgaatttccc agaagagaga atggttagac ttggaaaaga tgataatttc tgggagatag
    18121 gtacaggtcc ttgtggtcct tgctcagaaa tatattttga tagaggagaa gaatatggat
    18181 gtgacaatcc agactgtaaa ccaggctgtg attgtgatag gtatttagaa ttttggaatc
    18241 atgtatttac tcaatttgat agagatgaag aaggaaatta tagtctatta gaaaacaaga
    18301 atatagatac aggtatgggt cttgaaagaa tgggatgtat tatgcaaggc gtagacacta
    18361 tctttgaagt tgatacaatt aaatctatac tagaagcagt tgagaagtta acaggagtaa
    18421 aatatggaga gaatccaaaa aatgatatat caataagaat aataactgac cacataagag
    18481 ctgtaacatt cttagttagt gatggagtgc ttccatcaaa tgaaggcaga ggatatgttc
    18541 ttagaagact tcttagacgt gctgctcgtc atggtaagtt attaggagta aaagaattat
    18601 ttttacagaa actaatagat gaagttataa aagtaaatga taaagcatat ccagttttag
    18661 ttgaaaaaga aagttatatt aaaaaagtag ttggaataga agaagaaaaa ttcaatgaaa
    18721 ctatagacca aggaacagaa atattgaact cttatataga agtattaaaa aatgaaggaa
    18781 agactgtcct aagtggtcaa gaagctttta aactatatga tacttatggc ttccctatag
    18841 acttaactaa agaaatatta gaagaagagc acttatctgt agatgaagaa gcttttaatg
    18901 aagaaatgga aaagcaaaaa gaaagagcta gaaatgctag aggaaatatg gatggagaaa
    18961 gttggaagga agaccctcta tcaaaattag aatctacagt tgacagtaca tttaatgggt
    19021 atagtgaaat ttatggtgaa ggaactattg aagctatagt taaagatgat gagctagttc
    19081 aaagtgcaaa agaaggggat aaggtatcta tagtactaga taacactact ttctatcctg
    19141 aaggtggagg acaagttgga gactgtggat tgataactaa tgaaaactta gttttagaag
    19201 tattaaatac taaaaaaggt gctaataata gtataaaaca tataggtata ataaaatcag
    19261 gtagaataag taatggggac aaagtaaaga cactcgtaga tggagaaact agaatgtcag
    19321 ctgcaagaaa tcacagtgct acacatttac ttcataaggc attaagagaa gtattaggag
    19381 aacatgttaa tcaagcaggc tctttagtaa ctccagaaag acttagattt gatattactc
    19441 actttgaagc gatatcaaat gaagaattaa aagtaataga agaaaaagta aataatgtta
    19501 ttttatcatc tttagacata aaatgtgata ttatgaatat aaaggaagct aaagaaaaag
    19561 gagctacagc actatttggt gaaaaatatg gtgatgaggt aagagtagtt tctatgggag
    19621 attattctac agaactttgt ggaggaactc acttgacaaa tacttctcaa gtaggaatgt
    19681 tcaaaatatt atctgaaggt ggagtagcag caggtgtaag aagaatagaa gcaataacag
    19741 gaaaagctgt ttatgaatac ctaaaagaaa gagatggaat tatctctgaa gtttgtgtaa
    19801 acttaaaatc taaagaagat aatttaattc aaagaataag ttctttatta gaagaaaata
    19861 aaaacttatc taaagaattg catgatatga aagctaagat gagtttacaa tcagttgatt
    19921 ctatttttga ttctaaagtt gaagtaaatg gagttaattt gataactaat aaatttgaag
    19981 gtatggatat ggatacacta agagaaacag cagataatct aagagataag cttggttctg
    20041 gtgtagtagt acttgcaaat gtagtagatg ataaagttaa ctttgtagtt actgccacta
    20101 aagatgtatt agataaggga atacattcag gtaatattgt tagagaagtt gcaaaaatag
    20161 ctggaggaaa aggtggagga agacctaata tggcacaagc aggagctagt gatgtatcta
    20221 aggttgacca agctttaagt tatgcaagtg aggttattaa aacacaagtt aaataacatt
    20281 cactcaagga ggtttttagt ttggaaaaag atttagacta cactatgaaa tttgaaggaa
    20341 taccagaaga taggatgagt gtaggagata ctattgattt tgtatataaa gcattagttg
    20401 aaaaaggata taaccctata aatcaaatta tagggtatct cctttctggt gattctagct
    20461 atataactag tcataaaaat gcaagagcta taattaaaaa atttgaaaga gatgagatac
    20521 ttgaagaagt aatcacgcac tatctgaata gaaagtaggt caaataaatg agaaaaaaga
    20581 ttatgtcgtt gtttgcaata gtcattatga tttttgtatc aactgctagc tatggttttg
    20641 ctgaagaaaa aggcaaggtc atatttattg atatgaatag gaccaatctt ggaagtatga
    20701 tgaatattcc aatcttgaaa aatgagatag aaaagagagg ctatgtagct ctcatgaata
    20761 taagagggga tcaaggtact gatgatagaa ggtcttatgc aagtatgggt gcaggaacaa
    20821 gagctagtgt aactactgaa gactatatca attttcaaaa agtagaccaa gatactgcta
    20881 aagcttttga agtcgcaaca ggtcaaaaag ctaaagaaat aaatgattta ggcattaata
    20941 gatctataaa tgaaaatctt gagtatggag aatatggagc aaatttaggg tcattaggaa
    21001 aaactttatc agaaaatggt ttgaagacat ctgtaattgg aaatgctgat ataattgaaa
    21061 ataatcaatt agttagaaat agaaacttat gtttagtagc tatggaccaa tatggaagga
    21121 ttgattcagg taatattgat aatattaata agaaagattc taaaatgcca tttggaatat
    21181 ctacagacta cgataaatta ctagatgaaa ctaaaaaatc ctataaaaat agtgatgcta
    21241 tatttgtaga acttggagat acatatagat tagatatgta tagattaaat ctaaatgata
    21301 atacttatgg tacaatgaag aaaaatattg taaaaaatat aaataaatat cttgaacaag
    21361 tattttctat ggttggagaa aatgacactg tttatatagc aagtgcattt ccaagtgacc
    21421 tagattataa gaataagaga agactttctc caataataaa atttagtgga gaagggaaag
    21481 gtctactgga atctgccaca acaagaaagg ctggaattgt tgccaattta gatgttggag
    21541 tagatatact aggtacattt ggtcttgaaa ataaagagat ggtaggtaga agttatactt
    21601 tagtaaataa agaggacaat attagctatc taaaatcact ttatgaaagg atagtgtcta
    21661 tatcttcaat aaggtcaact attataaatg gctttgtagg cgtaatatct gcttcttggg
    21721 taattgccat gtttgcaatt ttctttagaa attatatgcc acataaagag cgtgtattca
    21781 aagtacttaa ggagtttata aaattaggca taataatgcc actatcattt atgttagctc
    21841 ctttgatgaa ttttaaaact ccaatttcaa tatctttggg aatatttgca actacgctaa
    21901 tattatactt gttaggtaga aaattattta aggacaatga tataaagcac atgggatttt
    21961 ttgcaacact tactatttta gtcatagtta tagattctgt atttggaaca tacttaatgc
    22021 aaaataacat aatgagttat gatgctgtca taggagctag atactatggt gtaggaaatg
    22081 aatatgaggg tgtaactata gcaagtgcag tatttggctt agcagtatta ttacattata
    22141 agaaagtatc taaattactt gttgtaattt tttcgttaat aatacttata actagtgcat
    22201 atccttcaat gggtgcaaat gttggtggag ctatatcaga atgtgtagca tatctgttgt
    22261 ttataatgct tatatatgac attaaaatag attttaagaa agctgtatta ttagctgttt
    22321 cagctgtagt cgtcgttttt gcatttgcag ctttagatat actttcaggt agtgaatctc
    22381 atttaggtat gtttgtacag agaattttcc taaatggacc aggagagata ttacaaacat
    22441 ttggaagaaa aattcaaatg aatatgcaac tagcacagac aagtgtatgg gtaaatattt
    22501 tacttgtagg aattggagta attgcagtac ttatatttaa accatctaga cactttagaa
    22561 agataatgaa taattttcca atcttgttta aagggtttat tgcatctatg gttggttgta
    22621 taattactct tttagttaat gattctggaa tagttgctgc atctacagca tctatatata
    22681 tacttatacc actaataata ataagtataa atatgataat atttgaaaaa aataactaaa
    22741 aaaattttta ctgttcatat aggatttaaa taaaaaatgc aatatgatta ttaatgtaaa
    22801 ctggatgctt acataaaaaa tcatattgca atttttatga ttgattatgc aaaattttat
    22861 gaagcaggaa gcttgtgacc ttagttatga gaggttcaga atatgtattt aatctataga
    22921 atataaatag ataaaggagg aactatgtta gatggaagaa taatgggtct tgatgttggt
    22981 gataaaacta ttggagttgc tgtgagtgat ttgatgggat taactgcaca aggtgtaaaa
    23041 acaataaaaa gagttggcaa gaaaaaagat attgaagagc taaaagcaat aataaaagaa
    23101 aagcaagtaa ataaaattgt atcaggatta ccaaaaaata tgaatggaac tttaggtcca
    23161 caaggagaaa aagtaatcaa gttttgtgaa ttggtaaaag cagaaacagg tattgatgta
    23221 gagttttggg atgagaggct ttccacagta gcagcagaga ggtctttatt agaggcagat
    23281 gttagtagac aaaaaagaaa aaaggttata gatatgttgg cagcagtaat aattttgcaa
    23341 ggatatttag attttaaaat aaattcataa aagaactata aaattgaata cttatgatat
    23401 ataatataaa taataaaaat aaaaataaat ttgaggtgaa tgacattatg gaagaaaata
    23461 taataaattt aatagacgaa aatggcgttg aaagccaatt tgaaataata ttaacattag
    23521 aagcagaagg aaaagagtat gcaatattaa tgcctttaga tgatgaagaa gctgaagaag
    23581 ctttaatctt tagaattgat gaggatgaag aaggtgaaat cttaatacct ttagaaagtg
    23641 atgaagagta tgaaactgtt gtagctgtgt atactgctat aatggaagaa gaaggtttaa
    23701 attacgacga agatgaatca aatggactta actagataag taattaagtt tgtatataaa
    23761 aatcaggtat ttattatatt tgctttaatt aaaccttatt aattgtaaaa gcatttatag
    23821 tatatatctg tttttttgtt ttttattaaa aatagcaaaa aatttatgaa ttttggtgaa
    23881 taatatatct tttatgggta tatatttata ttgtgagaat aaattaaatt tatttaaatg
    23941 tcttgacaaa aaatgaatta tcaattacaa tattatttaa taaagattat caattgaaaa
    24001 tagtaagggt gatgttatgg caaatacaat ggatttatta aaagacaagt taaaagaaac
    24061 tggttttaag attacgccac aaagaagagc aatagttgaa atattgctta aacatgacca
    24121 ttcacatcta agtagtgagg aaatctatga tttagttagg gttgactgcc ctgaaatagg
    24181 cttagctact gtatatagaa caatgcagct attggatgaa ataggattaa tatcaaaact
    24241 aaacttagac gatggatgta taagatatga gattagtcta cataaagaag actgtcataa
    24301 tcatcatcat cttatttgta aaaattgtgg aaagataatg gaagctaaag aagacctttt
    24361 agacaatata gaaaaagaga tacaatcatt gtacaaattt aaaatacttg atcatgatgt
    24421 taaattttat ggattatgtg acgagtgtaa tggagtaagc gattcagaag aataaacttc
    24481 tattgcttaa tgtcgatgga agaatagtat agaactttta tattctatgc tattcttcca
    24541 ttttttatta taaaccatct ataaaatgca aatatacagt tttcaaagtg attatagttt
    24601 taatattgaa aatataatta atcctataag tacaaaaatg aatgattttt ctatgaaatt
    24661 actaaatggt cctccaacag ttaaaaaccc taaagaaatt ttcttttcgc ttagcggata
    24721 aaaaattggt attccttgct ttgtaaataa atctcctaaa aacatatggc ttgcataacc
    24781 aatcgtacca taaaaagata aattattaat attagtgatt aattctatct gttttagtaa
    24841 aaaatatact attatagttg caaatatgct atgagaaaaa cttctgtgct ttaaccaggg
    24901 aaaaattgac aacattagac aaaaaattgt aaaatatatt tcaacttcta tgatgtataa
    24961 gcatatagct aatagtataa atattaatga tagaaaaact tttcttagaa atgtgtgggt
    25021 tagttttgct tcaataatta taattgcaac aaatgtaatt attgcgctta aataaaaatt
    25081 atcatttata tttattgaaa taaagaaaat aataatatta acagcatata taaaaattct
    25141 atatatgtat ttggatacat tgttttttag aatgaaattt gagaccatag aattagattt
    25201 atccaaatcg ggtagtacag caaaagtagc tgaaactaaa atatcaacta aagatatagg
    25261 aattttaaat attaaagatg tctggatagc tgttaagatt cctattgcac aatgagtttt
    25321 accacgcata aatcctccta aattatccga tattaaatca tataatattt agaggatatc
    25381 aaaataaaat aggtttgtca aaataatacg ttaaatgtat tttaaatata gcttaaaata
    25441 tgatacgaat tatggtacaa taagaataac tggtagcgag tgcgtgattt gatattattt
    25501 atgattgtag aacgtgttaa aaaattaaat agacagaagg agatgatgca attgttcaaa
    25561 aagaacacca acaagataaa ggtaatggcc ttgggtggac ttaacgaagt tggaaaaaat
    25621 atgactgttg ttgagtataa agatgaaata attgttatag atgctggact tagttttcca
    25681 gaggatgaaa tgttaggagt agatatagtt ataccagata taacttattt agtaaaaaat
    25741 agggataaaa taaagggtat atttattaca catggacatg aagaccatat aggagctctt
    25801 ccatatatat taaagaaaat aaatgtacct gtatatgggg caagacttag tataggtctt
    25861 atacaagtaa aacttaaaga acataagatg aataatgtta agttaaatgt tataggtcct
    25921 agacaagtta taaagcttga taatatggaa gtagagtttt tgaaaaataa ccatagtata
    25981 ccagatgctt actctattgc aatacacaca gaccaaggta taatatacca cacaggagat
    26041 tttaagattg acttgactcc tattgatgga gatgttatgg atatgcatag aatctgtgag
    26101 ttgagcaaaa aaggagttct tttaatgctg gctgatagta ctaatgcaga aaaaccagga
    26161 tttactatgt ctgaaaagac tgttggggta ggtcttgacg aattatttgc aaaaggaaat
    26221 ggcagaagaa ttatagttgc tacctttgca tccaacatac acagattaca acaaataata
    26281 aatactgctg agaagtttaa tagaaaagtt gctatatcag gtcgctcaat ggtcaatgta
    26341 gttggagttg caaaagaatt aggatatcta gatatttctg atgacatgct tatagattta
    26401 aatgatatat gtaagtatga agatagtgaa ttagtgataa taacaactgg ttcacaaggt
    26461 gaacctatgt cagctcttgc tagaatggct ttttcagagc acaaaaaagt agaaataaag
    26521 agtggcgatt tagttataat atctgctcat ccaataccag gaaatgaaaa attaatatca
    26581 agagtaatca atttcttatt tgaaaaaggt gcagaagtag tatacagtga tatagctgat
    26641 atacacgttt ctggacatgc ctgtcaagaa gaattaaaac ttatacatgc attagttaga
    26701 ccaaagtttt ttatgccagc tcatggtgaa tatagaatgt taaaaagaca tgctgaaata
    26761 gctgaacagc ttggtatgga taaagaaaat atttttgtca tgcaaacagg agatgtttta
    26821 gaattagata aaaactctgc taaggtagca aatcgtatac aaacaggaaa catattagtt
    26881 gatggtctag gagtaggcga tgtaggtaat atagttttaa gagatagaaa gcatttatca
    26941 gaagatggtc ttatgatagt tgtagttact atttctaagg atgagggtaa agttttagca
    27001 ggtccagata taatttccag aggattcgta tatgtaagag aatcagaaga tttaatggat
    27061 ggagcaaagg atataattaa gaatgttcta aatgaatgtg aagagaaaaa tattaaagaa
    27121 tgggcttatt taaagaataa tattaaagaa aacctaaaag aatatttata tcaaaaaact
    27181 aaacgaaatc caatgatact tcctatcata atggaagtat aatataaaaa aactataaat
    27241 ttgaaaatta gacctcttgt aatttttaaa gtaatatcaa aaatccaatt gatattatct
    27301 taagaattac aagaggtctt tttaatactt gatttaaaaa aatatatata ttacataaaa
    27361 taccaattat aacaatcttt tattagtaaa aactacttat atataaattt tactgatagg
    27421 aggattctta tggatgatgt ttcaagacaa aatgctataa aagctttaaa acaaacaaaa
    27481 atggaaatag caggagagta tggaatgaat tatgaagatg cttttgaaat aatagaaaat
    27541 gcgtctaata aaggtgtttt agaaggttac tttaaaaagc ttgaaaagaa aaaaaattta
    27601 ggtcaaggta tatctaggca tttagaataa aaaaagttaa atgtgagaaa ataagtttaa
    27661 ataaacttta agaggtgaca tcgatgaaaa aattagctag tactgctttg gctatattaa
    27721 ttgcgcttac tcctttaagc ttttcttttg caaataataa ggaaaatgct gatgccaacc
    27781 aactaaatat atcttcaaaa tcagcaattt tgatggatgt tggtagtgga caaattttat
    27841 atgaaaagga tgctcataaa aagcttcctc ctgctagtgt aactaaggta atgactatgt
    27901 tacttatagt agaagcttta gattcaggca aaattaaact tgatgatgaa gttcaagtaa
    27961 gtgaaactgc atctagtatg ggtggtagtc aaatattcct agagccagga gaaactcaaa
    28021 aggttgatac attattaaaa ggtatagctg ttgcttctgc aaatgatgct tgtgttgcta
    28081 tggctgaaca cttagctgga agtgttgaag ggtttgttga tagaatgaat gctaaagcaa
    28141 aagagttaaa tatgaatgat actcattttg caaatacaaa tggacttcca gttgcaaacc
    28201 actatacatc tgctcatgat atagctttaa tgtctagaga actattaaaa cacgaaatga
    28261 taagtaaata cttaactaca tggatggata aggttgtagt agggaagaaa caagttacag
    28321 taggtcttgc aaatactaat aaacttataa agcattatca aggagcaact ggtgttaaaa
    28381 ctggctttac tcaagaagca aaatactgtt tatcagcatc tgcaaagaga ggtaatactc
    28441 atctagttgc tgcaacttta ggagcagaga cttctccaga aagatttaac gacgcatcta
    28501 gccttttaaa ctatggattt gctaattatg aaagtgttaa gttatgtagt aaaggtgata
    28561 atatagcaac acttacattg gataaggctg atgaaaacaa agttaaattg gttgctaaag
    28621 aagatttaaa tgctcttatt aaaaaaggaa gctctaaaga atttgagaag aaaattgaaa
    28681 tagtaaaaaa tcctaagatg ccaattaaaa aaggaactgt tttaggaaag ataaagatat
    28741 gtaaagataa aaaagtaata ggcgaagtgg aattaataaa tactaaagat ataaataagg
    28801 caagctatct acaaatgctt caaagaataa ttgataacat gatataaata att
//
LOCUS       DAEMAE010000023        28108 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEMAE010000023 DAEMAE010000000
VERSION     DAEMAE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.023"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..1142
                     /locus_tag="KPH93_000852"
     CDS             78..1142
                     /locus_tag="KPH93_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1687687.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEVNLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKISD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKANNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1300..1986
                     /locus_tag="KPH93_000853"
     CDS             1300..1986
                     /locus_tag="KPH93_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687688.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINVFKQKVKAFLNHFTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKNILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLQYIKTVYGMG
                     YMWLGERNEF"
     gene            1976..3007
                     /locus_tag="KPH93_000854"
     CDS             1976..3007
                     /locus_tag="KPH93_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1687689.1"
                     /translation="MNFKKFSFNSIYLIIVVGIIFTVILFNISIYIITDDLKLVALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEYNIEIELEEETILSRINHR
                     LIRMYEVIQESRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQN
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSEIGKGS
                     TFSIFLPNK"
     gene            3089..3754
                     /locus_tag="KPH93_000855"
     CDS             3089..3754
                     /locus_tag="KPH93_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687690.1"
                     /translation="MSILQVINLKKYYGQNSNITKALDSVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3767..6183
                     /locus_tag="KPH93_000856"
                     /pseudo
     CDS             3767..6183
                     /locus_tag="KPH93_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(6361..7152)
                     /locus_tag="KPH93_000857"
     CDS             complement(6361..7152)
                     /locus_tag="KPH93_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1687691.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIGVFRRNTQSNENISITQGNAMDLSI
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSVFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLTTCEREDLVGVTSHAIDIFRK"
     gene            complement(7185..7772)
                     /locus_tag="KPH93_000858"
     CDS             complement(7185..7772)
                     /locus_tag="KPH93_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1687692.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSRSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGMAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(7950..9341)
                     /locus_tag="KPH93_000859"
     CDS             complement(7950..9341)
                     /locus_tag="KPH93_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG1687693.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIISPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCDTGIEKGLIGLMGGVIAAKGGTWGLAVGFILYFVLIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9508..10806)
                     /locus_tag="KPH93_000860"
     CDS             complement(9508..10806)
                     /locus_tag="KPH93_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG1687694.1"
                     /translation="MKVNVYNVESFVDEKLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEIDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASIYEQIKKIVVSQPHTYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLETLEILEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGVFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11167..12042)
                     /locus_tag="KPH93_000861"
     CDS             complement(11167..12042)
                     /locus_tag="KPH93_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG1687695.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMEIDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12224..12910)
                     /locus_tag="KPH93_000862"
     CDS             complement(12224..12910)
                     /locus_tag="KPH93_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG1687696.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGVLEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(12959..13492)
                     /locus_tag="KPH93_000863"
     CDS             complement(12959..13492)
                     /locus_tag="KPH93_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG1687697.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13536..14168)
                     /locus_tag="KPH93_000864"
     CDS             complement(13536..14168)
                     /locus_tag="KPH93_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG1687698.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14255..15337)
                     /locus_tag="KPH93_000865"
     CDS             complement(14255..15337)
                     /locus_tag="KPH93_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG1687699.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNIY"
     gene            complement(15470..16816)
                     /gene="pepV"
                     /locus_tag="KPH93_000866"
     CDS             complement(15470..16816)
                     /gene="pepV"
                     /locus_tag="KPH93_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG1687700.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIMGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDSEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(16885..18165)
                     /locus_tag="KPH93_000867"
     CDS             complement(16885..18165)
                     /locus_tag="KPH93_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG1687701.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEDIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDLDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYNDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18292..18552)
                     /gene="hfq"
                     /locus_tag="KPH93_000868"
     CDS             complement(18292..18552)
                     /gene="hfq"
                     /locus_tag="KPH93_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG1687702.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(18666..19601)
                     /gene="miaA"
                     /locus_tag="KPH93_000869"
     CDS             complement(18666..19601)
                     /gene="miaA"
                     /locus_tag="KPH93_000869"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG1687703.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIYEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQNQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(19639..21606)
                     /gene="mutL"
                     /locus_tag="KPH93_000870"
     CDS             complement(19639..21606)
                     /gene="mutL"
                     /locus_tag="KPH93_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG1687704.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMMTKTKDEMIGTKIYVEGGKIITKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGNDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCNNPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(21617..24460)
                     /gene="mutS"
                     /locus_tag="KPH93_000871"
     CDS             complement(21617..24460)
                     /gene="mutS"
                     /locus_tag="KPH93_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG1687705.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KKYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKIIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQKKAKEIKS"
     gene            complement(24623..25357)
                     /locus_tag="KPH93_000872"
     CDS             complement(24623..25357)
                     /locus_tag="KPH93_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687706.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKAYKDIEVLKDISIDVHEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLVL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(25326..26339)
                     /locus_tag="KPH93_000873"
     CDS             complement(25326..26339)
                     /locus_tag="KPH93_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1687707.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(26361..27131)
                     /locus_tag="KPH93_000874"
     CDS             complement(26361..27131)
                     /locus_tag="KPH93_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687708.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MNSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(27106..27396)
                     /locus_tag="KPH93_000875"
     CDS             complement(27106..27396)
                     /locus_tag="KPH93_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG1687709.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(27527..27626)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         9442 a         4511 c         3202 g        10953 t
ORIGIN      
        1 acgttattct tatataatta aattatacaa caataaagtt atatttttta aatatatttt
       61 tactaggggg aatagaattg aaaagggttg atgagagaaa tactatgttt gcaagagcaa
      121 attacaaaaa agatagtact gcttataatg actattataa gaaaaatcct gacaaaaaag
      181 aaattgatga tagtataaga aatagaccaa atttatgtag tgaaggaaca atgacttaca
      241 atgagttaaa ctctccaatg gcaagtagtg cattcgattt tttatctgat atcaaatctt
      301 tatgtgaagg aaaagtttct gatactaaag tagatgtaga tgcaaagtca atgacaaaaa
      361 aaataaaggg tcttgcaaag cagtatggag cttctgtagt tggtataact aaattaaaag
      421 actatcatat ttatacaaat aggggtagac atgaagaaaa ttacggtgaa gaagttaatc
      481 taactcataa gtatgcaatt gtatttggat gtgagatgga taaagaaatg ataaatagag
      541 ctccaatgat atgtgaagtt atagaaacat caaagtgcta tgtggatgca tctatagtcg
      601 gcatgatttt aagttactat ataagaaatt taggatatga tgctagaaat catatggatg
      661 ctaattattt agtaatgcct gtttttatag ctcgtgatgc tggtttagga gatataggaa
      721 gaaatgctat acttacaaat aaagattatg gctctagact tagattaggt gttgttacta
      781 cagatattcc tcttttagaa gatgagtatg ttgattttgg actagaagat ttttgtaagg
      841 tctgtaaaaa atgctctttt aactgccctt ctcattcgct ttctaatgat attaaaatta
      901 gtgatgatgg aaaatataat tgggtaattg aacatgagac atgctatatt aaatggagat
      961 atttaggtac agattgtggt atgtgtatat ctgcttgccc atttagccaa aacctcgaaa
     1021 ctataaagaa tactacttca tttaaggcta ataatgagct tattcaaaaa gctttagatg
     1081 agtatacttc aaaatttggt aaaagaatat ttatacctgg aaatccatct tggttgaaat
     1141 aattttattt aataaagaat ctgtcctaga atttgtcata ggacgggttc tttattaaat
     1201 atataaactt ttaaatactt tttctcgttt ataacaattc agcaatattt ttagtctata
     1261 attgacatta taaaatatta aactaaaagg agtaactgta tggaaaaaat attagtagtg
     1321 gaagatgatt ctatattaaa taaaactcta tcatataacc taattgaaga tggatatata
     1381 attacttcaa aatttacagc taaatctgca ctaaagtcta tttttgaatg tgaatttgat
     1441 ttaataattt tggatattaa tttgccagat aaaagtgggt ttgaattatg taatgaaata
     1501 aaggaaaatt acaatatacc aataattttt ttgactgcta atgatatgga atgtgatatg
     1561 attaaaggct atgaacttgg agctttggat tatataacaa aaccatttaa tatcaatgta
     1621 tttaaacaga aagttaaagc ttttttaaat cattttacaa caaaaacaaa acaagattac
     1681 tatagggatg gatatcttga aattaacttt tctgagttat ctgccaatat aaatggaaat
     1741 caaattatat ttacaccatt agaatatcgt actttaaaac tgcttactga aaatcctaaa
     1801 aatattttaa caaggaaagt acttttagaa aaactctggg atatagatgc taactttgta
     1861 gatgaacata ctttaacatc tgttataagt aggattagaa gcaaaattga aaaagacaat
     1921 cttcagtata ttaaaacggt atatggtatg ggatatatgt ggttaggtga aagaaatgaa
     1981 ttttaaaaaa ttttctttta attctattta tttaataatt gttgttggta taatttttac
     2041 agttatttta tttaatatct caatttatat aataactgat gatttaaaat tagttgctct
     2101 attattaata tatacaatag tactttgtgg atttatagca ttaataatat ttttacttca
     2161 taaaaaaata gttatatttt gttctaaaat atgtcacact ttagatagta tgatgaatag
     2221 tgaatacaat attgaaattg aattagaaga agaaactatt ttatcacgta tcaatcacag
     2281 attaattaga atgtatgaag ttattcaaga aagtcgcaat agtattgcta tagagaaagc
     2341 tgatttacaa gaactagttt ctgatatatc tcatcaaatt aaaactccta ttgcaaattt
     2401 aaaaatgatt aatactactc tactaaatca aacatttaaa acagaaattc aaaatgattt
     2461 tttaatggat atggaaaacc aattagataa attagatttt ttaatgcaat ctatggtaaa
     2521 aacctccaga ctagagacag gtataatcac actatctaaa aagaaaaatt caatttatga
     2581 aacaattgct acagcactta gtggaatttt gtttgatgct gaaaaaaaga atattgaagt
     2641 tacagttgat tgtgacccaa atttatatat aattcatgac aagaaatgga cttctgaagc
     2701 tatttttaat atattagata atgctgtaaa atacacaagt tctaatggta agatacgtgt
     2761 agctacagaa tgttgggtta tgtatacaaa aatagatatt attgataatg gaaaaggaat
     2821 atcagaaagt catcaagcag aaatctttaa aagattttat cgtgaagaag atgtacatga
     2881 catagaaggt attggaatag gtctctatct ttcaagaaaa attatatctc ttcaaggtgg
     2941 ctatattaaa gttacatcag agattggaaa agggtcaact ttttctatct tcttacctaa
     3001 taaataaatt tttaaatatt tcatttgtaa tgattctgca acattttggt tttatactgt
     3061 atttacaaga tgttaaggag gtattattat gagtatttta caagtaatta atttaaagaa
     3121 atattatgga caaaattcaa acattacaaa agctcttgat agtgtaactc tatctattga
     3181 aaaaggcgaa tttatttcta ttgtaggtac atctgggagt ggcaaatcca cgttgttaaa
     3241 tatgatggga ggattagata atcctacatc tggaaaggtt attataaaag gtaaggaatt
     3301 atcgactatg actgatgaac aacttacaat ctttagacgc agaaatatag gttttatatt
     3361 tcaaaattat aatcttgttc ctttacttaa tgtatatgaa aatattatct taccaatagg
     3421 attggatgga gagaccatag ataaggattt tataaatgaa attataaata tgctttctct
     3481 tgaagaaaaa ttatatgata tgccaagtaa tctttccggt ggtcaacagc aacgtgttgc
     3541 tatagctaga gcacttgtaa caaaaccagc tattatacta gctgatgaac caacaggtaa
     3601 ccttgatagt agaactagtt cagatgtact gagtttactt aaagttacaa gtaaaaaatt
     3661 caaccaaaca attgtaatga ttacacacaa taatgaaatt gcacaactaa ctgatagaat
     3721 tattcgtatt gaagatggaa aaattgttaa ctaggaggtg tagtacatgt ttagaaataa
     3781 taatcgcaca tttataagaa aaatatcttt aaatgattta aaaattaata agcttaaaac
     3841 atatctttca ggtatcatta taatgatttc tacatgttta ttattaacag tgaccttagt
     3901 ttcatataat gcttccgtag acttagttaa tgcatctcca tatcatgcta tatataaatc
     3961 tgtagatgaa aaaacaaaaa atatactata tcaagataaa aactttagta gtgtaggaac
     4021 ttacaaatta attggaagta ataaaaagac agattttatt atgtcgattg tttatgcaga
     4081 tgatacagca attaaactta tgaattttca gccattgaaa ggcaacctgc ctacaaataa
     4141 aaatgaaatt gctatttcag aaaaatattt acaggaattt ggtttagata aagatattgg
     4201 agatagcatt aaattaaatt attatgatga tattacaaat aaagaagttc aatgtgattt
     4261 tattattgtt ggatttctag aaaattatta tcaagataat gccaagcaat actatacagt
     4321 cgtttcaaat gattatttta agaatatagc ttccgcacct ttacaaaata aaagtaatac
     4381 ttttgacgag tcaatacctg atactgtaga tgtattagtt aaactatcag aagaaaatac
     4441 agagaaagat tcagcttcaa ttaagacaca gttaaagaaa attgctttat ctttaggtat
     4501 tgagaaatat aatatatact taaatgataa ctatattgaa tcaaatttaa ttgatggtga
     4561 gcagattatt acagttttag tagttggaat agttgttctc ttttctagtg tttttgttat
     4621 atatagtatt ttttatattt cagttgttaa ttcagttcag atgtatgcta agttgaaatc
     4681 gttaggtatg acttcttttc agttaaaaaa gattatttct ctacaaggaa atattttgtc
     4741 aattattttt attccattag gggttatagc ttcttgtata attgcttaca taattcaacc
     4801 attagcatgg caaatgatgg ctgatttatt tataatttta attttatcac tggtgatgtt
     4861 tttaactgtt agaatttctt tatttaaacc agttagaatt atttctaaaa tatctgctat
     4921 tgaagccatg caatatactg aaactaaatt tagaaaaaaa gtaaaaaatt ttagttatat
     4981 aaatataaaa aatctagcat taaagaatat cgagtcaaat agaaagaaaa atttgattgc
     5041 attaatatca ctaagcatta gtggagtttt atttatttca atctctaatc ttgctaattc
     5101 aatagatttc aaaaaacaac tatctcaaca atttgtctat aacgaagatt atataattag
     5161 cattaagagt gataacttat atgagcatat tacagaaatc caacaaaata atccactaac
     5221 agattctctt aagaatgaaa ttaaatctat tcatggagta aaaaaattaa ttgaatccaa
     5281 atcaattaaa agcaatatta tagaaccttg catcaaagat agagatgaaa aacaatttat
     5341 tactctaatt aaaggtatta cgcctgaact ttcaaaatca cttgaagaat atattgtaag
     5401 tggaaaaata gactatacac atttagattc agatagttta attataaata aatatagaac
     5461 aaaatcctat ggattaaatt taaaagttgg agatactgtt acatttaata tttttagtgg
     5521 aaatgatatt attaaaaaga aaatgaaagt tatggggatt attgacaatt ccaatactgg
     5581 aggtatgttt tttgcttcag acaaaacttt agaatctcta acgccatata atacaaattt
     5641 agatttttct attttaatag ataaaaagca ttctaaagaa gttgaagaaa agttgttaag
     5701 tataattcag aaaaattcaa atttaatgct ttattcatat gaagatgatt atagaatgat
     5761 tactagagca tttcaatata taattatagc ttcatacgtt tttgttggtt taatttcttg
     5821 ctttggtata ctaaatatga ccaataccct aataaacagc gttttaatta gaaaaaaaga
     5881 gtttgcctta cttcaagccg ttggaatgac aagaaagcaa ctgcgaaata tgctttatag
     5941 agaaggtcta aacataagta tcaaagctat atgtatttca agtatactag gttattttgg
     6001 ttcaaatcta ctttgcactt ttataaaaga tgtaattaga ttagacttta ttaattttaa
     6061 attttctata tttactattt taattttttc attcgtatta attggtatac aagttttagt
     6121 gactgaatta cttgtaagaa atatagaaaa aaatcactta cagaacgctt acgttctcaa
     6181 taagaatgta tataaaaaat atccattttt tatacaagtt ttaacataat tttttaaaag
     6241 ctaaagggat agaaaatttt gactttacat aattttctgt ccctctactt gtttttattt
     6301 tatacagata tacttcctta tatctccaaa catctccaga gacataaaca atcatattac
     6361 ttattttcta aatatgtcaa ttgcatgact tgtaacccct actaaatctt cacgttcaca
     6421 agtagtaagg tgatatttta aatacaacgc aaatgcatca tcatccatag agtctactgc
     6481 ttcacgcata tataaagcca aaccatctga tgcgacataa tgcagtctag ttacattaaa
     6541 tacagacatc aaatcatcaa tgttttcttt acgtacaagc tcaaacaaat cctttggctc
     6601 agacttagca gcaaatgtct gtgggtcaat caaacctttt tcaatgtatt cagatacatt
     6661 aatatttcct ctatggaacc cctcgtcaat gagacaacca tcagatataa catatgcagc
     6721 aaaaaccaca ccaccaggtt ttgttacacg aatcgcctca tgtaaagctt gttgcttatc
     6781 ttctttgtta taaagatgat atagtggtcc tagcaataac gtaatatcat atttattatc
     6841 tgggaaaata gataaatcca tagcattacc ctgagtaatg cttatatttt catttgattg
     6901 agtatttcta cggaaaaccc ctatattatg ttcgaccaat tccactgcgt caacatcata
     6961 tccctgacgt gccagtgtgt gagaatagcg accagttgca gcgccaattt caagcacaca
     7021 atcacctgat tttatgtact tatcaatgta atgcatagta gtgagaaatt ccaccattcc
     7081 atgttttaat gcaagtcgac tatcttcatc ataagtatta taaaagtcaa ttaagtattg
     7141 ttttgtttcc ataatttcac ctcttctata ttagttaata aacattaaat agacaaattc
     7201 caatctggtt tttcttgtct tataaaagct gttaatatat ttttcaacat tttttcagca
     7261 ctatatctat ctaatgttaa tttttttgtt gctgttaaga cagccatgcc atgagtataa
     7321 agaaataaat ctaaaaatat tacttttgca cattccaact ctattccaat gctttccgaa
     7381 aaaagtcttg cttttttttc attgtcaatt tctttataaa aatcatttgc ctcttccatc
     7441 atcaaatcta aatcatttat aaataacaac ttaaataaat gagtttcttc ttgagcaaat
     7501 tcaatgtatg aaagcggaag cagtaaataa ggacttacac ttctagaatt acgataatta
     7561 acaacatact gattatagta ttcatatgca aaagcaagaa attcattttt taattcattc
     7621 atgttttcgt aacaagtaaa aattggtcga gtggaacact gtaatttact tgcaatactc
     7681 ctcgcattta cagcttcaaa tcccacttct cttgtaatat ccaagactgt attcaaaatc
     7741 atttcttttg ttatctttac tttaggtggc atatatgttc acctctccat taaattttta
     7801 ataaaataca tatgttatat aactatagta atatatcaaa gaattatgtc aagaaaaatt
     7861 tttgcatttt tttgcaattc ataaaactga taaataaaac atgagttgca attaatctat
     7921 taatagataa cagcaactca cattttcctt taaatactct tctttttatc taatatctca
     7981 tatacacaat cttctattga attaatatct ttttcttttc tttttttaga atcaattaaa
     8041 acgaaataca gtataaaacc tacagcaagt ccccatgttc cgcctttagc agcaattaca
     8101 cctcccataa gtccaatcaa tcccttctca atacctgtat cacatagctc cattgatagc
     8161 tttgtacata aatatccttg aactaaaagt gttactgaca atgctaatgg tataagaggt
     8221 gtagcaatct gcgcaattgg ataaaaacag ataaaaattg ctgaaaccca tactaatgta
     8281 cccataccac taaaaacaga ctgcatggct tcctttccaa ccttgtacct ttgagcaaca
     8341 cttcctgtaa gtgtcgcagc taatggaccg cacattggta tatatggaga aatgattgcc
     8401 ataaaaatat ttcttatccc acttataaca ttagacctat ttgaattgaa atctatatat
     8461 tcatcttttc tattttcact ttcagaaagt actaaatttt cacctgttat aaaatcacca
     8521 aatgcaatta tgtatatcat aaaagccatt ggtatccctt gtataaataa cattacactt
     8581 ggaaaaccta ttccaaaagg agatactgta tttatcaaat ttttaaaatc tactatgtaa
     8641 actaaattat caaaactaaa tctaggaatt tctatttctt tactaaatat acctactatt
     8701 aaactcacta aaatagctga tactacacca tattccccta aatgaaataa aaatttattc
     8761 ttaactttta gagtatcaaa tctttcagaa aacattacaa aacaagcaac caaaattcct
     8821 actgttatag atataggata agaatcaaat ctccctttag gattaaactc tgatattata
     8881 gtcgatatac cgacacccat tagtattcca cctttaatag aatttggtat cttactaact
     8941 aactttccag ctacaccagt tatacccatt aaaataaata ttattcctaa atctatttgt
     9001 aaagctgtta atgcttgtat tctttcaatg cccatgctga actttaacaa atatgcagtt
     9061 gtaagagtta tagaagctgt aatccagcca ggtacaactg ggtctcccat aaaaacatgc
     9121 aagcagtata atattgagtt aatgatagcc atactccatg ccacttcata gggtaatccc
     9181 aaaatttgtg tactaagacc aattgtgcct aaacttgtca tacttgatat tatgccttga
     9241 atagcttctg gtttctcaaa tctgtaatgt ataaatggaa ttcttatctt gaacattcca
     9301 aattttatat gaggttgttc ccttctagcg agtctttcca taacttcact ccacctttca
     9361 ataactctta taagtttaca actttataat tgtttataaa ttatcaatgt cgttaaaatt
     9421 agtatataac atgcaaatta tttaaacaat aaattaaatt tataaaattg atagattttg
     9481 tgcatatata gaaagacctc ttcattttta gttatttaaa tcacttgata aaggtttttt
     9541 ataaaattca atagttctat tccatgattc aaactgtcct tttgaatttc cttctacagt
     9601 tccacctgaa gaaaaaaccc ccccactcat actcatacat aaagtctcct ttagaggcat
     9661 aatatatgga attggtaacg gatgtcccat attattgtat gttaataatt ctacgttata
     9721 tggataatta ttttcttcaa ggatttctaa tgtctctaaa caagcttcat aactattcca
     9781 tatgttatct tctttcccag caatcataag tatatctgct ttggaatttt ctatttttat
     9841 tctagcctct tttctattct ctgctctttt aagacttttc ttataagtac tattaaatcc
     9901 aaatggaata ccttttgata tatttttttt ctgttcttca aagaatatat cattgtctac
     9961 cttgatatat ggaaatggct ctcctttata tgaccatgat gaagtatcat ttccactcat
    10021 caatccatta agagcttgga aacaataagt gtgaggttgt gatactacaa tctttttaat
    10081 ttgttcatat attgaagcaa gtaaaaaggc caattctcca ccctttgatg ttccatgtac
    10141 aaatatttct ttagtactag taatttcatt ttcactaatc cactcaaata ctttttcaaa
    10201 atattctaat ggaacctctt ctaatttatc tggtaaccct tctaaagcaa agtatgcaac
    10261 tgttagtaca ttgaatcctc tagatgcaag tggagccgct aaaaggtcta gagcgtctaa
    10321 atttccatca gaaccaccta gcattaaaat tgttttatta ttagaatttt gtttataaaa
    10381 taattttcct ataaactcat cagatatgta ttttgtggta atatcttctg atttaaatat
    10441 tctctttaat tcaacttctt ttctttcagt agaagtttca aatatcatat tcataattat
    10501 tggtttatca atagatatat tttctgcaaa atttttacct tcagttttag atttttccaa
    10561 cgaataaatt aaccccatac aattatcagc cttataagta ccttctatag gttcaactaa
    10621 atctaaatct atttctcctt tttcatttga acaaaacact ccataagatg aaaattcttc
    10681 tcccttgcac catggaaatt ccattttcac acttatttta agtttagtat ttggaattaa
    10741 gtctgaaacc ataatcttaa gtttttcatc cacaaatgat tctacattgt atacatttac
    10801 tttcataatc tatttctcct tataataatt atttatgctt tatatataaa acagctttat
    10861 ctaatacagc ataaataaat ttttaaatta agacactatg ttgactattc tattttatca
    10921 acatatctca tctttgtcaa catattgtct tttctggcaa tttaaaatta tgtagtctat
    10981 atgaaatcca gttgattttt ggctaattaa agataggaat gctattaggt ttagaatata
    11041 tctattcaaa ataaaaaacc actcatttta agaatggttt ttaacataaa ctatttgtta
    11101 ttaataagga tattttttat ttaaatttat ataatcgaac atctaatatt taatgtttaa
    11161 taaaatttat gcatttgcaa aagtatatat ggcattaact cttgctacat ataaattctc
    11221 atcactacat ccaatatatc tagcatattt ttcaatcttt tcttttgcat tttcaacatc
    11281 attttcatcg atttccatta actcatttat ataaatatcc attagtgatg ctttatattc
    11341 tcttatatca tagcctaaaa attcagtcaa tctctcaatt tccttactaa aatctccatc
    11401 ttcttttacc aaaaattcgt atacatcttc tctatatgga tttaaagtta ttatttgttt
    11461 tactactttg cattcgtcag ccttcccaat aaaaccatct ttataattat taaataaggc
    11521 attagatttt tttatagatt cttcatcaat ataagaatgt accgcagtta tacgattttc
    11581 tataagggca tctacaacag cataatgaat tttaaaacaa tcattgtata aataattgct
    11641 cattttcttt attgttgatt ctatttctaa gaagctagca tgaccatttt ttttattttt
    11701 atttggtata ataaaagttt tattcaaatt attaaaaagt ctcttaaaat ctaaatattt
    11761 ttcacaataa acacttttaa aggtatcgat atctatagtt ataattccaa aacttactat
    11821 agtctcaatt cctttcttaa aagactcttc tatatatttt tctcctaaac ttaaacctct
    11881 atcagatatt tgctttagat tttgaaaact atttaaacaa tctctctcaa agtttttaga
    11941 tatttctaaa gcatataatt taaagatttt ccttatctta ttataattat tttttgtatc
    12001 atcaaatctg ataatagctc catttacatc aaaagtaatc attgagtttt aacctcccca
    12061 ttagtttcat caataaaata aatttaatat aaactttata atcataatat taaacttatt
    12121 tatactataa ttattaattt gatgataaaa gctattttcc ttctaaataa aaaatttgtc
    12181 taaagtttct aaaaaattta gacaaatctt atgttattac tctttaaatt attccaaata
    12241 cgccaaataa tattggtata aatatagctg gtaataaatt ggctactttt atattcgtta
    12301 atttaagtac atttagacca agtcctataa ttaataaact tccagctgct gtcatttctg
    12361 tgactacagg atcacttaaa aatccattta aaaatgatgc tcctacacat ataatacctt
    12421 catacaaaaa tacagatatt gcagaaaaca ttacacctat acctaaagaa gcagtaaata
    12481 taattgaagc tattccatct ataacagatt ttacaaataa agtatcatga cttcctgtaa
    12541 gaccactttc taaggaacca actactgcca tggctcctat acagaataat aaactagatg
    12601 atataaaacc ctctgctata gaatcttttt tatttccttt agaaaattta ctttgtaaaa
    12661 aagcacccaa cttatttacc catttgtcaa tatctattaa ttctcctatc aaagcaccta
    12721 cagctacgga tattattgtc actaatgtat tttttccttc tagtactcct gatataccaa
    12781 tgtacaatac acataaggca attccattca ttatagtatc acttactctt tgaggtaatc
    12841 ctccttttat tataagacct actatacatc cacctgcaat tgctagacta tttgctatta
    12901 ctccaaacat tttttctccc ctcgattagc taattgaaat ttatcctgct attaaaacct
    12961 aaacatcaat actttgtatt ggatatttta aatattccaa aaattcttca aaaccttttc
    13021 tagatatatt tacggtttta gtatttatgt taggatgaaa atttaagtaa tcttctttaa
    13081 ttacatcttt atctatgtaa agctcaactt ttctttctgt atcatttatt aatgaaaaag
    13141 gatttacact tccaggaaga agtttaagta cattgtatag tctttcaggt gaagcaaacg
    13201 ataatctaga acttcctatc ttatctttaa gtgagtttaa atcaacttgt ttttctcctt
    13261 taactacaat aagataatac ttatttcctt tggcatttct taaaaataaa tttttacatt
    13321 gacacccctt tgttattcca tctaaaatga ctaattgctc tgctgtatat acagcttcat
    13381 gctctactac atcatattca acatttaatt tatccaaaat atcatatatt ttagtttcat
    13441 tcatgctaga ttcattttca tcttgttctt ttttattatt taaatcactc atacttttct
    13501 ccttaaacac ttcattataa tatcattgtg agatattatt ctactatagc tattgctctt
    13561 actggagctc catcagcatc attatactta aatggtgctg ctataaatag aaatttttct
    13621 ggaacatttt ctaaatttgt aagattttct atgattattt ttcctttctc aaataaaata
    13681 tgatgcactt caaatactga tgtgtcatat ctatccactg atagcatatc tattcctata
    13741 ccttttatat tagtattagc tatatagttt gcagcttctt ttgttaatgt aggataacct
    13801 acatagtact gctttttatc ccaaaacttt gaccaacctg atttaaatat tataaaatca
    13861 ctatttttta tttcatcttc atattttttt agatattcta attctatatt ctcatttccc
    13921 ttttcaagtt ccaatacata agctttccca acaaaatttt ctatatccaa cttatctagg
    13981 gtctcacctt tagtatacat atgtcttggt gaatccatat gagttccatt gtgagaaaat
    14041 acactaatta atgtttcttg ataaccattt tcttcaataa tagctacttt cttgatatct
    14101 ggtctattag gctctgcata tacaggcatg tcattatgtg tcacatgagt taaatcaaaa
    14161 accttcatct ttatataccc cctatgtttt tattttctat tatataccta aattcatttt
    14221 atttctatca gattttattc cattatatac catactagta tatatttttt ataaacctca
    14281 agggatttac cgaaaaatct tggaaagaat tatcagtaaa tataacatct tcacccaaaa
    14341 aacgtttata ccttccttct tctttcttta gagtaattct ctgtttttgt gaatgattat
    14401 taaaaaccat tatcttaaga ggaggttttt ttctattagt ctgcactctt tttaacatgt
    14461 attttatgtg catatcagct tcaggaaatg aatatccaca aaatattaaa aggtcagtat
    14521 ctcttaaagt tttttctgtt tcattccaaa cattacttaa aaatatgttt gacatattct
    14581 taaaatatgt tggtggcact ataataggtt ctgttaactc accacactct aaacattttg
    14641 tctctgaact attttcaata agcttcatga cgccaccttc atgtggtgta agagttacac
    14701 tattacaaat ggggcaataa agccaattca atgacccatg tatcttatat aatttgatta
    14761 atggttcttt tggttttctc caatttaact cctctttaaa attggtaaac tccacaccat
    14821 aatcaagcat aattggcaaa tcattcttat ctcttaatcc agtaactgta ttgtctatat
    14881 gtatatcata atttgcacta ataaaagtag tatcaagaag caaatcattt tccaaaagat
    14941 tttcaactaa aagtttatga tatttattat ctgttctagg tgtattatga attgcttcag
    15001 ccattagcag tattaaatat tgtcttaaaa aacgtatact atcacttctg ttatttagat
    15061 tttctagtcc aaaatttcta aatgcttctt ttctttgttc tgctaagtct aaaagaccta
    15121 aaacttcttc aaaggtagga aaatttgcat tatctatatt atcttttact atatcaatat
    15181 tgaacatctg tttaaagaaa atatacaatt ctctattcat tttggtatta taatttgtgg
    15241 gatttagttt tttaaaatat tcgctaaaaa gttcattttg aataggtaga ttctctgctg
    15301 ctgaagctcc tgctcctaaa aatattgcta ttttcatata gttcctccta atataaatat
    15361 tactcctatt ttcatatcca ttaaatatcc atatcattta ttttatgata caacaataaa
    15421 gctgtctttt actagaaaaa atcctattac aagacagcct tatattatat taaatcttta
    15481 ataattcgta aatagaaaga gcatatattt gagtagcttt tataatatcc tctactatta
    15541 agtattcatt agcttggtgt tctgtttctg gttttcctgg aaaaatagct ccaaatgcaa
    15601 cacaattatc taaagctctt gcgtaagttg ctcctccaga tgaaagtggt gttccatcaa
    15661 gaccagtttc ttcttcatat acttttctaa gtgtcttaac aagaagagta tcttctggaa
    15721 catagattga atccaagaaa tcatattctt cataatttaa attgtattta tctgtcattt
    15781 tctttaactc ttttacaaaa tcatcttttt tgtatgttac tggaatcctt acatctattc
    15841 ctgcaaattc tttttcgcta tcaatagtta ctctacctat atttactgtt agtttacctg
    15901 atacatcatc cttgcaatta ggaattatat tgtttccatt agcatcttca ccaataactt
    15961 ctgctaaaaa cttaattata ttagaatcta tacctatatt attaagagct atacaaagtc
    16021 tagccactgc atttatacca gtatcacttg ctgctgaatg gacacttttt cccattatac
    16081 agattttatt accttcaaca gtatactcaa aatttaattt atcaagttct ttttttaatt
    16141 tatcagagta ttttccaata tagattgctt tacctggaac tgcatttaaa gcctttccaa
    16201 ctgacaactc tatatcactt ttgctatcac aagtcaaatg aacttgtaaa agacctttct
    16261 ctgcatttgt tattgggaat ctagaatctg gagtaaatcc atagtttggt atttcttcat
    16321 tattttcttt atatttattt atacatctcc aaagattttc ttcatcagcc ccaaaaatga
    16381 atcttattct cttattaaag tctacattta aatccataag agctttcaca gcataaattg
    16441 ctgatattgt aggtccttta tcatcttgtg ttcctcttcc atacaattta ccatcttcta
    16501 aaacagcttc aaatggagga taattccagc tttctaaatc accttctgga accacatcaa
    16561 cgtgaccaag tatacctatc attttttcac cttgtcctat ttctgcatat ccataatatc
    16621 catctttgta tactgtctta aatcctagag atttacataa gtctaatata cctcttaaag
    16681 ctttatctac attttcacca aatggacaat tttcagttgc ttcagatata acactaggta
    16741 tttttacact ttcttgtatt gaagaaatca tttcatcttg tagagaattt actttttctt
    16801 taatttgctc tttcatatta tcacccttca attttcatca taatatacta ataaatattt
    16861 tgacctttta aaaaaaaaat atgcttattt tttcagtttt tttataaatt caatcgcttt
    16921 taaagtcccc atttttgaat ggtcaaatgt aagaccacct tggaaataaa cattgtaagg
    16981 aggtcttatt ggagcatctg cacttaattc tatagacgaa ccttgtataa atgctcctgc
    17041 tgccattatg acttcacttt catatccagg catgtcccaa ggaacaggtt ttacataaga
    17101 atcgactggt gcagcttctt gtataccttc acaaaaactt attacttcat cagcattatt
    17161 aagtctaata cattgaataa tatcacttct taagtcattg tattttggta atacatcata
    17221 gcctaatttt tcaaaagctc ttgagcaaaa gatagctccc attacagcct gagatactat
    17281 ataaggtgcc aagaaaaatc cttgaagtac atttctagtt gttccaaaag taagaccaca
    17341 ttcttttcct attccagggg aagtcattct ataagatata agttcaatca aatcttttct
    17401 accagctata tatccacctg tcaatgcaag cccaccacca ggatttttta ttaaagaacc
    17461 agccataaca tctgctccga catcagtagg ctctttggta tctaaaaact caccataaca
    17521 attatcaacc attactatag cttctggttt aacagatttt attacttcta tagcttcttt
    17581 tatatcacta attgaaagtg attttctcca agaatatcct tttgaccttt gtatcataac
    17641 aagtttagtt ctatcattta ttttgttttt aattccttct aaatctaagt ttccatcttc
    17701 taaaaaatct aaatcatcat atgtaacacc atactctttc aatgaacctt tttcttctct
    17761 tatgcctata actccctcta aagtatcata tggtctacca gtaacagata aaatttcatc
    17821 tcctggtcta actatacctt gtatacataa agtaagtgca tgtgtaccat ttacaattat
    17881 tggtcttaca agagcatctt ctgtattaaa tactttagaa taaatttcct ctattttttc
    17941 acgaccaata tcattatatc catatcctgt agtccagtta aagtgcatat cacttagttt
    18001 agattcttgc atagctttta aaactttata ttgattatat tctcttattt cttttatttc
    18061 ttcaaactta tcttttatat cttccattat ttcttgcgat agtttaaaag tatcatcatc
    18121 tattccatag tagtctttta ataattcttt tgtctcatta agcattttat cacctgtctc
    18181 tttattattt tatttaaaca tgtaaaggag actattttac taaaacaaat aaattatagt
    18241 ttataagtta gtataagtct cctctcaatc atcttaaatt aaattaatta tctatctgtt
    18301 gttattatta ttgttgtttt gattttgatt tgttagatta atatactttc caggttgtat
    18361 agttgataca gcatgtttat aaatcatgtt ttgttgtcta ttatcccctt ctattaatat
    18421 tatataacta tcaaacccct ttacaagccc tttaacttgc actccattaa ctaaatatat
    18481 agtaacaggt atcctttctt tccttgcatt atttaaaaac aaatcttgta aatttaaaac
    18541 tgtatttttc attagatttg taccctccca agattaaatt gaacttctaa aatattccta
    18601 ccttatattc tatataagtt atgacaaatt ttcaatatta agttgtatac ttaaaaattt
    18661 atatattatt ttatagaatc tttaatatat aagattattt cattttttaa ttcgtctata
    18721 ttttcatatt ggtctaaatc aaaccatttt gctgttttat atcttttaaa ccatgttatc
    18781 tgacgttttg cataccttct agaatctctt ttaattattt caatagcttc ttcgtatgta
    18841 tattctccat ccagatactt taatatctct ttataaccta taccttgcat tgatatcata
    18901 tcttttttaa atcccatact taaaagtttt tttacctctt caaccaaccc atttttaagc
    18961 ataatatcaa ctctcatatt tattctttga tacaaatgtt ctctatctct atttagaact
    19021 attattattg gttgatattc ttcattaaat tttaaatcac ttgaaaaatc attcattttc
    19081 ttaccactta gacaaacttc aagagctctt atgacccttt tagtattgtt tttatggatt
    19141 ctattagcag attcttcatc aagttctttt aacttattat gcatgtaatc tattcccttt
    19201 tcagcaagct gattttgaag ttcttctctt aattcattat tggcatcaga ttgtgcaaaa
    19261 tccatattat atattaaaga gtttaaataa agtcctgtgc ctccagttac taaaacacat
    19321 tttccctttt catttatttc atatatatat ttttttgctc tttgttgaaa ctctgaaact
    19381 gaaaatggat agtcaggttt aacttcatct ataaggtaat gcttcacgcc ttgcatctct
    19441 tcctctgtaa cttttgcact tccaatatcc atatattcat atatttgcat agaatcagct
    19501 gaaattatct ctgcatccat atcttttgct aattttattg acaaatctgt ttttccaaca
    19561 gcagttggac ctgtaagtat tataagtggt atttttttca taacattcct ttccatacat
    19621 tttaaatatc caaatatttt acataattct tttaaacatt ttttctattt ccgtcttaga
    19681 tatttcgacc attataggtc ttccatgagg acatgtaaat ggattattac acttttctag
    19741 ctgttccaat agacttttta tttcaatatc atatatttta tcatttgctt ttatagcaga
    19801 cctacatgcc atagatgcaa atctctctcc ttttaaatca taattacttg taatttcttc
    19861 tatattatca attatttgaa gtataaattt ctcagtttca ggtactccaa atatagtagg
    19921 aacacatctt accattatat gattattgcc aaaaatttct agttcaaaac caaatttcat
    19981 aaataactcc aagttatttt caatttgaag catatctaca tttgatactt caataaccac
    20041 tgggtctaaa agtatttgca tattgatatc ttgtctatag aacttctcca tatatctttc
    20101 atatagtatt ctttcatgtg cagcatgttg gtccaacaaa tacatagaat catctttgct
    20161 caaaattata taagtattaa atacaactcc aattacagaa tatccatata aggaaaactt
    20221 gcgttttgaa ctacacaagt attcttcttc actgtcctta atactatctc ctaagtaata
    20281 atttccttca tttttaattc catcaacttg aaactcttct tgtatattat catttgcact
    20341 atcttcactt agataattaa tgttttttac atcatcttct acacttgcat ttaaaacttc
    20401 acttactgat tgaaattcat ttattggttt ttcacttgat aaagtcacaa cttcaataac
    20461 ttcatcatta cctttagtaa tattactatt tttaggagta gattctaata aatcattatt
    20521 tcttaattta taatctgtac ttttttctct actgttaata gcgattctag gttgtgtttt
    20581 tttatcacta taagtagcat atttcccaat aagatttgaa tgtattaact taacctttaa
    20641 aaaatctctg agttctatat acacttcttg ttctttttca aatttaattt caagtttatt
    20701 ggggtgtata tttacatcta tacatgatgg gtctacttct atatttaaaa agcaaactgc
    20761 atgtttacct attggaatta tagacttata actttctgtt attgcatcta tgattatttt
    20821 actttttaca aatcttttat ttatatagat atgttgtaag tttttattag aacggtatat
    20881 attgttatta cctatatatc cattcatttt aaagtgattg cacttaaatt ctacatctat
    20941 tatattttcc gttatttctt ttccatatat acttctaatg gtgtttacaa gttttccatc
    21001 ccctggagta tttagcatct gtttattgtt atttgtatat ttaaattgaa tgtttggatt
    21061 acctattgca agtttattta tcaaatcact tatgtttata gtttctgcat gagtagattt
    21121 taaaaatttc tgtcttgctg gtgtattaaa gaaaatatct tttataatga tggtagttcc
    21181 attagtagaa cctattggtt cttttgttat tatttttcca ccttcaacat atattttagt
    21241 tcctatcatt tcatctttag ttttagtcat catttctaat tttgatacag ctgaaataga
    21301 tgctaatgct tctcctctaa aaccaagaga gtataaatca tacaaatcat caattttttt
    21361 aattttacta gtagcatgtc tcaaaaatga tttttcaact tcacttgaag gtattcctat
    21421 tccattatct gtaatcttaa ttagactttt tcctccatca attatatcta tggaaatttt
    21481 atttgcacct gcatcaatgg agttttcaat aagttctttt actacagaag aaggtctttc
    21541 tacaacttca cctgctgcta tcttatttat agtcaaatca tctaggatgt taattatatt
    21601 tttcattttt acacctctaa gattttattt cttttgcttt tttttgaaga ttataaagag
    21661 agtttatagc atctaatgga gtcatgttta aaatatccag atttaaaatt tcttctgata
    21721 gtatatctct atttacacta tcaaaagaga tttgagttaa ggctacttct tttacagaat
    21781 cttcttgatt aattttctct ttatcatcat tttgcttcat taaagcaact tcatcattta
    21841 atgctttaaa tttagaattt aatttatcat aatccttttt tatatgtttg tagtctttta
    21901 ctataagttc atgttctatt tttagagatt catatttatt cttaaaatta ctttgattta
    21961 ccttaactaa ttcttcgtct aaattactat taattatatt attattttct ttatctccat
    22021 ttattgcaac actgttgtaa acatgatttt tttccaagtc tttaagtata tattttgctc
    22081 tttctatgac ttcgtctggt aatttagcta atttagcaac atatattcca taactcttgt
    22141 ctgctccctg gggaattatt tttcttaaaa atattattcc ttccccatct tctttgactg
    22201 ctattgaata atttttaacc tctttaaact cttcttctaa atcagtaagt tcatgataat
    22261 gtgttgcaaa taatgtctta catctaatat ttttttgaat atattccact attgaccatg
    22321 ctaaacttat cccatcatag gtactagtcc cacgacctat ttcatctaat ataactaaac
    22381 ttctctctgt tgcatttttt aatataaggg atacttcgtt catctctacc ataaaagtag
    22441 attgaccttg agataaatcg tcactagctc caactcttgt aaatattcta tctaatattg
    22501 gaatatcagc agactctgct ggtacaaatg agcctatatg tgccataaga gctattatag
    22561 cagtttgtct catatatgtt gatttacctg acatattagg gccagttatt atgttaataa
    22621 tgttttctcc acgatttaaa taagtatcat ttggaacgaa attttcctct cctactatat
    22681 tttcgacaac aggatgtcta ccatttctta tatcaagttt attattttca tttattgctg
    22741 gttttacata gttatttata tgtgcaactg ttgcaagaga cacaaaaaca tctatattag
    22801 ctataatctt agctactttt tgtattctat ctatgttttt atatattgta tctcttatct
    22861 ctacaaatat ctcgtattct aaagatttaa tcttttcctc tgcatgtagt atcttttctt
    22921 ctatttcctt taattctgga gttatatatc tttctgcatt actaagtgtt tgctttctta
    22981 tatatgtctc atctagcttt gcctgtttaa aattggcttt tgttatttct atataatacc
    23041 caaaaacttt attaaatcct atttttaatg atttaactcc tgttttttct ctttccctgt
    23101 tttctatttc ctttacaaga aaggctccat tcttagaaat ctctcttagt tccttaagtt
    23161 catcactaaa atctgattta attatatttc catcttttat ggttattgta ggttcttcta
    23221 gaatagcttc atctattaaa ttgtatatat catctaattt atccatttct gaaacatact
    23281 tttttaggat ttttgcattt gataaattta tagtatcttt taaattaggt aacttttcta
    23341 tcgaattttt taagtgaatt aactcttttg gtgttactct ctcaaatgct atctttccac
    23401 atattctttc tatatcgtat atatttttaa gtatatcatt taggtcttct cttaatataa
    23461 agtcatcttt tatttcttct attacatcca atctattttc aattttagat ttatttatca
    23521 aaggttcttc tacatatttt cttaaaagtc tacctcccat tgctgtagaa gttttatcaa
    23581 gtacatgcaa caatgaacct tttttcttat ttcctcttat agtttgagta agttctaaat
    23641 tagttctagt aaacatatct aaaaccatat attctgatga attgtatata tttatattat
    23701 taatgttaga cgttatttgt ttttgagtat tgtatatata attcagaaga attgataagc
    23761 ttgactttat taggccttta tcatcaaact ttagtttttg tagataaaca tctggaaaat
    23821 attcttttaa tatatttata tctaaatagt tatcactaaa actttcattt atgtatatat
    23881 tacttacagt agctatatct ctaagctttt ctataaagtc taaatcattt ataattattt
    23941 cagttggatg gatttttgca atttcttcaa ttaccttatc ttcatttaaa catgttgcat
    24001 tagtttctcc tgtacttata tcaacataag ttagacctat attagtacca tccttataca
    24061 aagataataa ataattattc tttttatttt caagtaaatt tccatctaaa actgtaccag
    24121 gagtgatgac tcttataacc tctcttctaa caattccttt tgctgtagat gggtcttcca
    24181 tttgttcacc tattgctact ttatatccat tttcaactaa ttttgatata tatgaattgg
    24241 ctgagtggaa aggaacacca cacataggag cacgttcttc taaaccacaa gcttttcctg
    24301 tcaatgctat ttcaagagct tttgaagcta ctaatgcatc ttcaaaaaac atttcataaa
    24361 aatctccaag tctaaaaaac agtatacagt ctttatatct attttttact tcaagatact
    24421 gtttcatcat aggagttaat ttattcatat ctatttgcat tatcaaacct ccttcttttc
    24481 taattaaata ttatatcata gtagatgatt attattaaca caatatataa aattttgaca
    24541 tataactaaa aacaatccaa aatgtgtact cacattttgg attgctaagt ttaattctta
    24601 ttaaataaaa atatatataa ttttacaata aagatattat ttctttttta agtttaatga
    24661 tattttcaag gttatcttca tttaactttc tactatctat aacaaattct ttctttacta
    24721 ttgatggttt ttttgaaata acataaattc tgtttgatag cattatagct tcgtcaatat
    24781 catgagttat aagaagtatt gttgaattta cttttttctt aacatctaaa agccattttt
    24841 gcatggatgt tttagttaat gaatcaagtg ccccaaaagg ctcatctaaa agcattatgt
    24901 cattagaatt tataaaagtt ctcaagaaat tagctctctg tctcattcct ccagaaagtt
    24961 ctgaaggata tttatcttca tatccgctta atccaaatac ctcaaaataa ggttttacca
    25021 ttgacctagc tttactcttt ttttcttttt ttaaaaccag tggtagtgac acattatcta
    25081 ttatagtttt atatggcaag agtaaatctt tttgatacat ataactatat ttaccattta
    25141 tattcacttt tccactatca taatctgtaa gtttagttat aatattaaaa atagtacttt
    25201 ttccacatcc acttggccct aatatagaaa caaattcacc ttcatgaaca tcaatgctta
    25261 tatcttttaa aacttcaata tctttataag cttttgatac attttctata gatactttta
    25321 cttttttatt gtggtaaaaa ttcatttgtg taagcatcac ttgccttcat atccttatct
    25381 attaatttat attcttttaa gaatgatgta tagttgttcc atacactatc tttcatttct
    25441 ccccatcgtg gtgcatcatc tttgtacttg cttgctaaat attcttgact ctttaaagct
    25501 agtttttcat ctacttcagg tgcatgtttt actaaaattt ttgctgattc ttctggattt
    25561 tttattgcat attcataacc ttttgttgta gcttttaaga attttttagc aagctctgga
    25621 ttatctttaa gtacagtttc attagaaatt ataagtggtg tataataatc caatctctca
    25681 tctaaatctt ttactggtat aaagtttaag tcatatccaa ttaaatcagc tttaactgca
    25741 tcccaacctt caaatatcca tgcaaaatct acagttttca tagctgcaaa gaaatcatct
    25801 tgaccagtat tgactatttt taacttatta aaatcagctc catttttctt cataactgct
    25861 ttaaaaacag cttcttctga tggagagccc caaccaccat aagtttttcc ttcaaaatct
    25921 ttagctgttg ttatattctt ttcttttggt gatgcaaacc cagaagtatt atgttgtata
    25981 acagttgcta cagcttttat tggaagtgga tcttcactag tctttgcata agtaacttgt
    26041 tcttgatagc ttataccaaa gtcagctttt cctgttgcta ctaaagtttc tgctccactt
    26101 tcaggtggtt gtactatttc aacatccaaa ccttcttctt tatagtaacc cttatcaagt
    26161 gctacaaata atccagtatg atttgtattt ggtgtccaat caagcaccat agttactttt
    26221 ttagaagaag tatctttatc attgtttttg tctttattat tatttgaaga acatccagtt
    26281 attgttgcca atgtaaaaac acttactaaa cctaatattc ctaacttttt aaaattcata
    26341 attgcctccc tcaaatacct ttaatctatt aatctatgtc ttataacaac tttttttaca
    26401 gcctgtacaa ttcctacaaa cattaaacta aatataacta ctaatatagt agatgcaaat
    26461 actttatcaa gtgcatatgc actttttgac ctaagcatgt aaacaccaag tcctttcgtt
    26521 ccacctagcc attctgcaac agttgctgcc attacagcat acgttgcaga tattttaagt
    26581 ccagaaaaaa atttatccat tgccatagga aatttaaggt gaatgaatgt attaacttta
    26641 tttgagttca tagtcttaaa taaatttaaa taatctttat caatattctc aatgccatct
    26701 acaaaactta ttaatatagg aaaaaaacaa gtaagcatta ccatcaatac ttttggcatt
    26761 gttccaaatc caaaccatat cataaaaagt ggtgctataa ttattgtggg tatcatctgc
    26821 gtaaccaaca taataggata aagacatttt tttattattg atacaaaatc catcaatatt
    26881 ccaattacca atgatattat aattgcgact ataaaaccaa gtattgcttc tcctatagta
    26941 accacagcat gcatggataa attttgataa tctttaaaaa acacattaat tatatctact
    27001 ggtgtgggta gtatatactg aggaacttct aaaactccta ccatagattg ccaaagaata
    27061 agtataaaca aaaatgttgc tattggatat atactatctt tgactttatc tatgatttct
    27121 aagtttttca tctatagtca gacctccagc tttttcatct actctagcta ctgttatgac
    27181 tctttcacat ccaaggttaa aagggatttt atgtaccttt tttaataatt caaaaacttc
    27241 atcaaaatca ccttcaacag tagtgctcat tgctcctatt tcacatttaa gaccactttt
    27301 ttcaatttca gcaatacaag catctactac tttatacatt tcttcttcac ctttgtaagg
    27361 tcttagtggc attactgcta tgtctgcgat taccatttaa atcactcctt taaaaataat
    27421 taatacctac taggtatatt ttgttttttg ttaaaaaatt ttattactaa aaagatatta
    27481 tatttaaata aaagaaaagt cttatccaaa ataggataag acttattttt atcttaactg
    27541 gtctccctac actggcatta tccaaacagg ttcaaagggt cgagataaaa tctcctctca
    27601 gcacattagc tccccgaaat atattatatc ttttctactt gattatacta cacttatcat
    27661 attttaacaa attttttatt tatattagat attttatcca ttcaaaaata tctaatataa
    27721 attgcacatt attacatcaa ataaattatc ttaaaacagt aatttatcag tatcttccaa
    27781 aactagatta actgaattat ctagttttta ttttgctatt tcaagcatat ttggatgtat
    27841 atcaattcca tacaaatatt gtactaaatt tctgttttca ctcatctaat ttgcaatcgt
    27901 tacagattca ggttagacat agcttttaga ctctacttat ctttatctac aaaaaatcct
    27961 tcaataaatt cattaataat attcattaat ttaaaaataa acctctatct gaatttttaa
    28021 gatacaagcc tttcaggagt atcttttgtg agaatataaa aattactcgt cattatcatc
    28081 aaaatcctca gcaaccaaca ttttagat
//
LOCUS       DAEMAE010000024        28030 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEMAE010000024 DAEMAE010000000
VERSION     DAEMAE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.024"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(95..1102)
                     /locus_tag="KPH93_000876"
     CDS             complement(95..1102)
                     /locus_tag="KPH93_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG1687710.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1330..2214
                     /locus_tag="KPH93_000877"
     CDS             1330..2214
                     /locus_tag="KPH93_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1687711.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKGENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDEYFK"
     gene            2416..3966
                     /locus_tag="KPH93_000878"
     CDS             2416..3966
                     /locus_tag="KPH93_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1687712.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSNLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3995..4630
                     /locus_tag="KPH93_000879"
     CDS             3995..4630
                     /locus_tag="KPH93_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG1687713.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKFSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWVFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYKLCKSFRIK"
     gene            complement(4741..5001)
                     /locus_tag="KPH93_000880"
     CDS             complement(4741..5001)
                     /locus_tag="KPH93_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG1687714.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            5218..6501
                     /gene="hflX"
                     /locus_tag="KPH93_000881"
     CDS             5218..6501
                     /gene="hflX"
                     /locus_tag="KPH93_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG1687715.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6509..7186
                     /locus_tag="KPH93_000882"
     CDS             6509..7186
                     /locus_tag="KPH93_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG1687716.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDKFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7272..7715
                     /locus_tag="KPH93_000883"
     CDS             7272..7715
                     /locus_tag="KPH93_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1687717.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8020..8664
                     /locus_tag="KPH93_000884"
     CDS             8020..8664
                     /locus_tag="KPH93_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG1687718.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEAVEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIILGEEFYLSEQNGEILI"
     gene            complement(8750..9127)
                     /locus_tag="KPH93_000885"
     CDS             complement(8750..9127)
                     /locus_tag="KPH93_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG1687719.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGVF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9127..9513)
                     /locus_tag="KPH93_000886"
     CDS             complement(9127..9513)
                     /locus_tag="KPH93_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG1687720.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9692..10447
                     /gene="nadE"
                     /locus_tag="KPH93_000887"
     CDS             9692..10447
                     /gene="nadE"
                     /locus_tag="KPH93_000887"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG1687721.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10530..11249
                     /locus_tag="KPH93_000888"
     CDS             10530..11249
                     /locus_tag="KPH93_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1687722.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            12025..12819
                     /locus_tag="KPH93_000889"
     CDS             12025..12819
                     /locus_tag="KPH93_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG1687723.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            12913..14253
                     /locus_tag="KPH93_000890"
     CDS             12913..14253
                     /locus_tag="KPH93_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1687724.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14390..15622
                     /locus_tag="KPH93_000891"
     CDS             14390..15622
                     /locus_tag="KPH93_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG1687725.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15662..16804
                     /locus_tag="KPH93_000892"
     CDS             15662..16804
                     /locus_tag="KPH93_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG1687726.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            16815..17609
                     /locus_tag="KPH93_000893"
     CDS             16815..17609
                     /locus_tag="KPH93_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1687727.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17620..18642
                     /locus_tag="KPH93_000894"
     CDS             17620..18642
                     /locus_tag="KPH93_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG1687728.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            18741..20117
                     /locus_tag="KPH93_000895"
     CDS             18741..20117
                     /locus_tag="KPH93_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG1687729.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20447..20791)
                     /locus_tag="KPH93_000896"
     CDS             complement(20447..20791)
                     /locus_tag="KPH93_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687730.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21279..22289
                     /locus_tag="KPH93_000897"
     CDS             21279..22289
                     /locus_tag="KPH93_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG1687731.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     DICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     IF"
     gene            22425..22913
                     /locus_tag="KPH93_000898"
     CDS             22425..22913
                     /locus_tag="KPH93_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1687732.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23089..25017
                     /locus_tag="KPH93_000899"
     CDS             23089..25017
                     /locus_tag="KPH93_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1687733.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNNRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKEKTFTS"
     gene            25216..25626
                     /locus_tag="KPH93_000900"
     CDS             25216..25626
                     /locus_tag="KPH93_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG1687734.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            25648..25950
                     /locus_tag="KPH93_000901"
     CDS             25648..25950
                     /locus_tag="KPH93_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG1687735.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26128..26823)
                     /locus_tag="KPH93_000902"
     CDS             complement(26128..26823)
                     /locus_tag="KPH93_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG1687736.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATRLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILTS
                     HNPIFIKYYELIPKTIFSNQYFGFKYRNSLKDMISHHNNIIIAFDSKDSNLGYTSMYN
                     HLEDVIQLFQHDD"
     gene            complement(26849..28027)
                     /locus_tag="KPH93_000903"
     CDS             complement(26849..28027)
                     /locus_tag="KPH93_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1687737.1"
                     /translation="MSNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIGIII
                     GAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIANILS
                     GISSSTWISYMVHYTNFFSMEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMTALC
                     IMSVLSSVIGTILALNIKNYNFKKMPEKISIKKYLSICKDKRLIFFSFMALIQQGIQM
                     STTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSSFASTKFCIKYGPKFWIPLVFI
                     LVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGFFQA
                     IYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAMVASILSYTYYKKVDLTLNYDIIQS
                     "
BASE COUNT        10768 a         3128 c         5116 g         9018 t
ORIGIN      
        1 atatatttgg tctgtaggtt aaataaaagt acctttagac caaatatatt attaataact
       61 taataacatc ttaaaactta tttatttata ttatttacaa catccattta ataaatacac
      121 cataacctac agctgggtcg ttttcaattg catgtgacac tgctccgatt attttattat
      181 cttgaactat tggagtacca ctcatacctt gtacaatacc accagtttga gctaaaagct
      241 ttttatctgt aattttaatt ttaaaacttt tactttctgg atgtttttgg ttttctatat
      301 ctataatttc aatattatat tttttacact tattaaagtt atcttgtaaa attatttgag
      361 cttttcctaa tttaacttca tctaaagatg ctactttata tttttttaac ttggaaatat
      421 caaagttatt aatttgacct cttattccaa aatcagtatt ttctgtaaag ctaccaataa
      481 tattttctcg ttttgcattt atacatccaa cactaccttt ataagatttt tctatattaa
      541 ggtgcttagt tgtagaaatt gaacctgttt taataggtat ttttcttgca ttaccaacac
      601 ttattggatg acctacagct ccaaactctg atgtatctgg attgatatac gttaaagtag
      661 caaatccaga aagtaaattg ttaaaactaa ttttttctaa tatgtgtttg gtagttttca
      721 ttgttataag ttgtccacct ctatttaata aaatagatac ttgtttttct tctggtaaag
      781 catttaaggt attggcaaag tcactatatc catcaacttc agcattattt acctttacta
      841 tagcatcacc caatctaaat ggagatgctt ctccaggatt tctaactatg atatttttta
      901 attctgcgtc aatttgaagt acatttccaa ttggtattaa atattcaggt tcattttttt
      961 gtaaattttg tgcataaatt aaattatttg agaaaaaata taataaaaat aaaaatgtta
     1021 aaacaattgt aaattttgac ttagctaagt tggttttttt acttaaagta ttatttttaa
     1081 aatgaaaatt tttaagttgc ataaaacacc tctttcttat taaattattt taataataga
     1141 attgaagttt tcttgctgct tctatattat gttgtgcaag aattacgttt tgatgcttgg
     1201 aaaactgact caataacacc ttagttttca taaaaataaa taaaataaat agtgtatatg
     1261 ataaaataaa tacatattca tataaataaa aataaacaat aactgtctag cataagaaag
     1321 gagcaaaaaa tgaaaaaata tagattgtta gttacttgta taattgccat gggaatcata
     1381 attataggaa catcaaatcc agttagcaat ttttacaaga ctacaaattt gattagttca
     1441 ggaaatatca caaatgaaaa agcagataaa ggtgaaaatt caaataaaaa taataaagac
     1501 aaaaatgaag acaaatctaa ttctaaaagt aataaagata agtcatcaga ggacaaatcg
     1561 tcagataata aaaatgaaat tcaaaaaaat aaaaaacttt taatttgtat agatccaggg
     1621 catcaaggaa aaggagatag caatttagaa ccagtagctc caggttcttc ctctaaaaaa
     1681 gcgagagtat cttctggaac agagggtatt gctacaaaaa aaccagaata tgttttaaac
     1741 ttagaagcat ctcttgtttt aaagagtata ttagaatcta aaggttataa tgtaataatg
     1801 actagagaaa cacatgatgt aaatataagt aattcagaaa gagcaatact tgctaatgat
     1861 aaaaaagcag atatggttgt aagaatacat gcagatagtt taaataattc ttcaaaaact
     1921 ggagcatcta tattgattcc tgaaaaagat ggaaaatata cagctcctat atatgaagag
     1981 agtaataaat gtgctgaatt tatcaaacag aatatggaac aatcaggtat acaaatcaat
     2041 ggaatcgttc aaagaggtga tttgactgga ttcaactggt caaaagtgcc agctgtatta
     2101 gtagaaatgg gatttatgag taactataat gaagaccaaa tgatgtcaaa tccagactac
     2161 caaagaaaaa tgatgcaatg tatagctgat ggattggatg agtattttaa ataagatatg
     2221 aatagagaaa aagttaggct ggtattattt tcctaatttt tatctctatt ttttattgta
     2281 cataacacaa agatgaagtt tattttaggt taaattgtcg taaatataac taaaaatatt
     2341 gggaatactt gatttaagtt taaaaagaat atattatagt atagaatgtg taaaattttt
     2401 ttgagaggtg ttgaaatgct agtagtagaa aatgtgagtc atggatttgg tgcgagaaca
     2461 atattagaaa atgtatcctt tagacttaga aaaggtgaac atattgcact agttggtgct
     2521 aacggagaag gaaaatcgag ttttttaaat ataataacaa agaagcttat gccagatgct
     2581 ggaaatataa aatggtcttc aagagctaca gtaggttact tagaccaaca tacggtatta
     2641 agtaagggaa aaactataag agaagtgctt agagaggctt ttaagcatat gtttgactta
     2701 gagcaagaaa tgattgctat gtatgacaaa atgggagaag ctagtgatga tgaaatgagt
     2761 aatcttcttg aagaaacagc agagatacaa actatacttg aaaatagtgg tttttatatg
     2821 atagatgcaa agatacaaga agttgcaaat ggtcttggtc ttggagaaat tggtcttgat
     2881 aaagatgtaa ctgacttaag tggagggcaa agaacaaaag tacttcttac taaacttcta
     2941 cttgaaaatc caactattct tatattggac gagcctacaa actatttaga cgaagagcat
     3001 ataacatggc ttactaagta ccttcaagag tatgagaata gttttgtctt agtttctcat
     3061 gatattgaat ttataaacaa tacatgtaat gtaatatatc atatggaaaa tggagagtta
     3121 aatagatata aaggaaatta tgatgaattt gtaagactta atgatataaa gaaacgtcaa
     3181 gaagaacaag cttatgataa acaagttgaa gaaagaaaaa gactagagga ttttgtagct
     3241 agaaataaag ctagagtagc aactagagga atggctaaca gtagacaaaa gcaacttgat
     3301 aagatggaaa tattagaaag accaaaagaa aaaataaaac ctacttttgc ttttaaagat
     3361 gctagagcag ctagtaaaat aatttttgaa acagagaatt tagttttagg gtatgatgaa
     3421 gcattgacaa aaccacttaa tttccattta gaaagaggaa aaaagattgc cttaaaaggg
     3481 atgaatggta ttggaaaatc tactctatta aaaactttac ttggaataat aaaaccattt
     3541 gaaggaaatg ttaaattagg tgactatttg gaagttggat attttgaaca agagagttca
     3601 agagaaaata gtaatacacc tatggatgaa gtttggagtg aatttccagg gcttacaaac
     3661 tttgaagtta gacaggctct tgcaaaatgt ggacttacaa atgaacatat aactagtcaa
     3721 atgagagtat taagtggtgg agaagctgct aaggttagac tttgcaaagt aatgttaaaa
     3781 aatataaatt tcttagtatt agatgagcct acaaatcatt tagatgttga ggcaaaagat
     3841 gaattgaaga aggctatcaa agagtttaag ggaacagttc ttttagtatg ccatgagcca
     3901 gaattttatt cagaaattgt tgatgatgta tggaatatag aagattttac aactaaaatc
     3961 gtgtaatgat ttaacttatt gctgggggga ttttatgaaa aagtttctaa atgtactttt
     4021 ttcagtttgt attagtattg ttataatagt aggtgttatt aattttactg ttggatttaa
     4081 acaactttat tattttgaca ttgattattt aaatatatca gagttatctg gtttatctaa
     4141 agatgatata aaattgaact acgattatct aattgattat aatttaaata agaatgtgtc
     4201 tgagttcaag ttaccaacat taaaattttc tccacaaggg aagatacatt ttgaagaagt
     4261 caggaatata tttcaaaata taaataaatt agctaaatta ttattggtag tttctttagt
     4321 aggaattata ttgagtgtaa aaaataaaaa tataaaaatc ttgaaaacta cgtcaataac
     4381 acttataatt atgccgttat tattaacagt tccaatttta ttaaattttg aaaagtcatt
     4441 tattattttt cataaattat tatttagaaa tgattattgg gtatttaatc cgaatttaga
     4501 cccagttata aatatattgc cagaggaatt tttttttcac tcaggaatga tgattttaat
     4561 attaattttg ctagcaagta tcttactatt tgtaatgtat aaattatgta aatctttcag
     4621 aataaaataa gataacaaaa acgaattata ttaaagttta tacaataaaa atagctatgt
     4681 aataatattt agactaagag tagtcaataa atatttatgc atagctattt ctaatagaaa
     4741 ttacttttta ctagtgattt taactaaatc ttgaaatttc cccataatgt aattgtaatt
     4801 atcttttttg tgaggtaaca tgcaactact gcaatctttg atacctttat ctgtatactt
     4861 aaagtttcca ccacaatttt ctcctaaagc atacaaagga caatagcaaa atagacagtt
     4921 aaactcttct ggtttattag ttttatgaca agggaaaaat tcgcaatctt tgtgattaaa
     4981 aaacttataa ttttcgccca tattttcctc ctaattaaat aaaatttaaa ttttttatac
     5041 tatataagat tttggtgata atataattat actaaataat tttatgaata gtcgataata
     5101 tagtattatt aatgatagta ttaatggtat aattagtcaa cagaaaaata atgtaaaaat
     5161 aatgtaagtg tattttgtaa ttagaatatg ttttataaca aaaaatgagg tgattaagtg
     5221 aaagaaattc aagaaaaagc acttcttgta ggtctgaatt tgactacaat agtgaaaaaa
     5281 aatgatgata tagacactag tgaatctatg gaagaattaa aagagctaac aaaagctgct
     5341 ggggcagaag ttgtaggtag cttaattcaa aataaacact ctgtagatgc agcttattat
     5401 ataggaaaag gtaaagtaga agaaataaga gcctatagtg actctttaga tgctacttta
     5461 gtaattttta atgatgaatt atctggtgct caaatcagaa atatagagaa tgttgttgga
     5521 agaaaagtaa tagatagaac tactttaatt ttagatatat ttgctcaaag ggcacttagt
     5581 aaagaaggta aattacaagt tgaattagca caattaaaat atagattgcc acgactttat
     5641 ggaatgggtg gtgagatgag tagaacaggt gcagggattg gtactagagg tcctggagaa
     5701 caaaaactag aaatagataa gagacatata cttaataaag cagcagatat aagacgtgaa
     5761 cttaaagaag taaaaaagaa tagagaaaca cagagagtta agcgtttaaa gtcaaatata
     5821 ccgattgtag cacttgttgg gtatacaaat gcaggaaaat ctaccctgtt gaacgaatta
     5881 ataaagacac acaaggatta tgaacaagaa aaagaagttt ttgtaaaaga tatgcttttt
     5941 gcaactttag atgtaacctt gagaaaggct cttttgccaa ataaaaaaga atttttagta
     6001 gtagatactg ttggatttgt aagtaaactt ccccatgatt tagtagaagc gtttaaagca
     6061 acattggaag aggtacagta tgcagattta atcttacatg taattgacgc aacaaatact
     6121 agttatgaat tgcaaaaaag tacgactgaa ggtgtgttaa aagaacttgg tgtaaatgat
     6181 aaaaaacata tacttgtata taataaagtg gacaagctcg aattggatat atatcctaaa
     6241 agtcaagaag atattgtgta tatttctgca aaacaaggca taaatatgga taagttatta
     6301 aatatgatag aaattgcttt aatggaaaat acttattcag taagcttgat gttaccttat
     6361 gagagaggtg atatatttag tagaataaaa gataaatata atgtggagaa ttttgagtat
     6421 ggggaaagtg gtataacgct agatgtagat ttagatgaag aagattttaa tatctataga
     6481 gagtacatac tagaaaaata ggttgaatat ggatataaac tgggaaaata tagataatct
     6541 tgaagattat tttataacct atcttcttta taaagaatct aaaacagtat ctcaaattag
     6601 taaaattaga aatatttcaa gtactgaagc aagtgagcaa cttatacaag caaagttaaa
     6661 aataaaggaa atgcaaaaag atgattttga ggcatctaag gatattttgg ataaattttt
     6721 agaactagat aaaattaaga gacttgattt tatggatagc ttagatgacg aaaaaatggt
     6781 atactttaag agaaaggttt tcaaaagaat ccttgtagaa aaaaatgctg aagatttaat
     6841 tgttttaatt tgggcgacag gtgagctaaa agatgataag tttttgaaat tgcttcatca
     6901 gctcacaaac cacagacact cagatattag aagaataact tattcagcaa tcagaaagat
     6961 tgaatcacca agtagtagag aagtactgca aaaaggtctc tacgacaaaa atgcacagac
     7021 taggcagtac tgtgcaaaag ctctttcaaa attgggagat gaaaacagtt taaagatttt
     7081 acagcagttg aaagacaaga ataagaattt tgaaaaagaa tatgtgctta gagcttatga
     7141 cgaagccatt aagtcattgg aaaatgcaaa aaatttgaaa aagtaatttt ttattaaagc
     7201 aatttgttta cttaatgatg tatttctaaa gtttaacaag gtaataaata aattaatggg
     7261 aggggtttca aatgatagta agacgttgtg caggtggagt agtgttttat gctaataaag
     7321 tacttattgt aaaaaatgat aggggtgaat ggacacttcc aaaaggaaag atactaggag
     7381 ggggacttcc atatgagagt gctgttcaaa gagttaaagt agaaacgggc atagatgcta
     7441 aaatgataga tgtggctggc gatactatgt atgaattttt ctcaagaagt agacaacaag
     7501 aagtttgtaa tgcaataatg tggtatgtta tggaagcttg taacactgat tgtgtacttg
     7561 ctcctgaatt tcaagaaggt ggattttata aagtaaagga tgctttagaa atgttatcac
     7621 atcataaaga gcaagcttta gtagaagtat cttataaaaa gtttaaggaa ttaaagaaat
     7681 tagtttcaga gggcgatgat gttccagcaa attaattgtg aatttagagg attctgagga
     7741 agggatagcc ccttcctttt ttaattaaag cagttttaag cgtaaattat atagtctctt
     7801 taaattatga tttatcaatt tagagcttta gtagtttata tttaaaatca gtaagatata
     7861 tttaaaataa aaattaaaaa gtatacaatt ttattatata attgtcttat gatgttgatt
     7921 aaagaattat taaaagtcca tttattttta aatagaataa ttttataaat atatatttga
     7981 tgtgtgtatt agaaaatgat taaaatagga ggaaatacaa tgagcaatta caggacatta
     8041 catgaatttg gaacagatga aataacgatt gaaaagtctg tttttattgg atatgctaag
     8101 cctataaaga gtgaagaaga agcagtggag tttataaatg aaataaagaa aaagcataaa
     8161 gatgcaactc acaatgtgtg ggcttatact gtaggtaaaa atatgaatat acaaagatat
     8221 agtgatgatg gagaaccaca aggaacagct gggataccta cactagaggt tataaagaaa
     8281 gaagatttga gagatgtagc ggttgttgtt actaggtatt ttggaggaac aaaattaggt
     8341 gcaggtggtc tagttagagc ttatacaaaa ggtgctaaac tgggacttga agcaggtaag
     8401 ataatttata aggtaatgta tcaagaagtt aaggttaaga tagactatac tcaacttgga
     8461 aaagtgcaaa atgagttaat gaatttgggg tattttataa aagatactgt atatgaggat
     8521 aatgttgaga tagtagtgta ttcaagatta gaagatgtag agaaattatc agaaaaaatg
     8581 attgatataa caagtggaac aggaaaaata attttaggag aggaatttta tttgtctgaa
     8641 caaaatggag aaattttaat ataatctaaa aggttattag aatagtcaat acttcaaata
     8701 gtcaattggt atttttaagt gaaaagccat taaattgatt taatctaaat caatttaatg
     8761 gctttttatc agattttgca cagaatcctt tttttacttc aaataaaccg actaaaagta
     8821 aaaatacccc aaatattttt tttaatatat ctgaagacag ataattagct aatagtgaac
     8881 caaatacagc tcctaaaacc ccaaacacag ctataggtat aaccaattta aaaattacat
     8941 ttttattttt tatatgtata gcaagtgcta taagtgtcat agggatagat gaaagtaaat
     9001 ttacgctttg tgctatttta gtgtctattc cagcaagtaa aacgagtgca ggtataagga
     9061 ttgttcctcc acccattccc attcctccaa taattcctgc aaaaaatcca attagagcaa
     9121 ataacattaa aacaccatcc ttaatgctgc aattatcatg attattccaa aagaaattct
     9181 aagaaatttt ccagtaacct tatttagcaa tttagctcct ataataccac caagaatact
     9241 tccaactgca actttaattg ttaagttaac gtcgtaagta cctttagata catataaaac
     9301 agaacttgct gtcgttaaga atattattat agaaagagca gttgcatgag atttatgttc
     9361 ttctacctta acgatgtcat ttaatattgg aactaaaaga gtacctcctc cagaaccaaa
     9421 aactccattt ataaatcctg taaaaactcc aattatcgta ttttttaagt taaaatcaaa
     9481 gtattttttc tttaaatcat cagttttttt cataaaaatc accttctgaa atagttttac
     9541 caaaataggc aaattatatt tatataaaat aagattaaat ataatctttc atgtttgctt
     9601 aattttgcat aaaaaaaaac atattaaaac aacatattaa tatatggtat aatatattaa
     9661 aacaaagtag caaatttgag aggagataac aatgtcaaat ataaaaatac aaatagacaa
     9721 aactgtagaa tggcttataa ataaggttaa tgaggcaaac gcaaaaggat taattgttgg
     9781 tgtatctgga ggaattgatt cagcagtagt tgctaattta attaaaaagg catttccaga
     9841 aaactctatg ggagttataa tgagcataaa aagtaatcct caagatagag aagatgcact
     9901 taaggttata gagggatgtg atatagaata ccttgattta gaccttatag aaccacaaag
     9961 tgctatatta gacatggtag taggaaattt aaaagataaa catttatata gagaagaata
    10021 tttaaagatg acagatgcaa acttaagagc aagagtaaga atgagtacaa tttatacaat
    10081 tgctaacaat ctaggatatc tggttgttgg tacagacaat gctgcagaaa tacacacagg
    10141 atattttact aaatttggtg atggtggagt tgatatttta cctattgcaa atttaactaa
    10201 aggagaggtg tatgaatggg caaaagagct tggtgttcat gaagatttaa taaacaaagc
    10261 tccttcagca ggtctttggg aaggtcaaac agatgaagat gaaatgggaa ctacttacaa
    10321 tatgatagat gctgtgttag aaggtagatt ggatgaagtt cctaaaagag accaagagat
    10381 aattgaaaga cttcatagat taagtgaaca caagagaaaa acgcctgcac aacctcctaa
    10441 gttctaattt actatttata agatttgtga taaaatttac aagtgatgaa aattagaata
    10501 attaattgta cgtattaagg agtggaaata tgggacgtat aggtaatata attaatagaa
    10561 aaggtaaaca agatgctcaa agagcaaaga tatttactaa acatgctaga gcaatagcag
    10621 ttgcagctaa agaaggtgga gcagacccag agtacaatgc agcgttaaaa acagctatag
    10681 aaaaagctaa ggcagataat atgccaaatg ataatataga tagagctatc gctaaaggtg
    10741 ctggagcagg tgctggagaa gactatgaaa ctatagttta tgaaggatat ggacctggtg
    10801 gagttgctgt tatagtagaa actttaactg acaacaaaaa cagaactgct ggtaacgtaa
    10861 gatattattt tgacaaaaac ggaggaaact taggaactag tggatgtgtg tcttttatgt
    10921 ttgataaaaa aggacaaata ttagttggtt taggagatgg agtttcagaa gaagaattga
    10981 tggatgttgc attagaagca ggagctgaag atttcataac agaagaagat ggttatgaaa
    11041 taataactac accagaagat ttttctagtg ttcgtgatga gttaaaagct aaaggatatg
    11101 agtttatatc tgctgatgtg aagatgatac ctcaaactac gactgtattg actgaagaat
    11161 ctcatttaaa aatgatgaat aaattagttg atatgcttga agaagatgat gatgttcaag
    11221 atatatatca taactgggaa gtagaataaa tgttataagc atattgaaat ttataagtat
    11281 tgaatcaatt ttaacatgtg ataaatttta tggaataaga aattactaaa atagacatac
    11341 gcacctctaa cctatatttg cataataata tgtaagcata gataaggggt gtttttatat
    11401 ttataggtta agaaatttaa aaaaatggag ttatttgatt tagaataata tcaaaggaga
    11461 ttaacttaac attgcaaatc taatctaaaa ttgtcagaaa catttaagat aggaaaagtt
    11521 acttagacac taagtctttg tggcttttcc tatttttgtt aagaaatttc tactattaga
    11581 gcagatatat actgaactaa agtattaatg gtttaaatca atagaaaata aattatctac
    11641 tatgttgaat aaagattcct tatatcagag tgaaaataaa cttatagaga tgttatttaa
    11701 attattttaa tgaaaatatc aaaaatgaga ctgtattctc aaaaatgaga ctgtgttttt
    11761 taagtatttt ctcgaaaatg agacaaaaat attgttttat gggttctatt gaagaaaaat
    11821 tcttatgtga attacaattt aatcgatttt aaagttgaaa attattaaaa atttagtgaa
    11881 atttatgtat atttcgttta ttttattaaa aataacaatt ttttactaaa aataaagaac
    11941 ttcaatggtg attggcatag tacttgctat tttatattaa caagaaatgt agaaaaaata
    12001 acaatgtata atggggggaa tatgatggaa tctgtaatgg aaaatttgaa caatcttaag
    12061 gtggaggtta aggacaaagt ctgtgttatt actataaata gacccaaagc tttaaatgct
    12121 cttaatagtg acacccttag ggaactatca caagttattg atgtagtatc agaaaatgag
    12181 gcaatattag gagttattat tactggagaa ggtaaagttt ttgtagctgg tgctgatata
    12241 cgccagatgc aaaactataa aagtgaggaa ggcagaaaat atgcaggtta tgctcaggga
    12301 atttttgata aaattgaagc tcttgagaaa acagtaatag cagcagttaa tggatatgca
    12361 ttaggtggtg gatgtgaact tgctatgagt tgtgatatta gaattgcatc agaaaaggct
    12421 atatttggac aacctgaagt taatttagga gttattccat gttttggtgg aactcaaaga
    12481 ttatcaagac ttgttggaac tggaatagcc aaagagttaa tttttacggg gagacaagta
    12541 aatgcagaag aagctaaatc tattgggctt attaataaag tggttccaag tgatttactt
    12601 ttagaagagt ctatgaagat gatgaaccaa atagttgaaa aagcgccaat agcaataaga
    12661 tatgcaaaag ttgttattaa caaagggata gatatggatt taaagaatgc tcttgaacta
    12721 gagaaagata ttgcaggttt aacttttgct acaagagata agcaagaagg aatgaatgca
    12781 tttatagaaa agagaaagcc agtttttgaa aataagtagc tattataact aaattggaac
    12841 aatgtagaat atattcaaaa tctaaatttc gtgaaaaaat acatatagtt taattttaaa
    12901 aggagttaat ttatgaatac gggagtaatt ataagtttag tagggattat catagctcta
    12961 tctcttctag ttgtaatggt tatgaaggga ataaatattt ttattatagc aattgtatgt
    13021 tcatcattag ttgctataac aggaagtcta aatgtgtatg atgcacttaa ggtggattat
    13081 atgacagggt ttgttggatt tcttcaggca aatttcttta ttttcctaac aggtacatta
    13141 atgggaaaaa taatggagat tactggtgga gcaaaatcta ttgctaagat gattgtaaga
    13201 tggattggga aggataaagc actactttct attccaatag cttgtggaat attagcctat
    13261 ggtggagtaa gtgttttcgt tgtaagtttt gcagtgtttc cgatagcttt agaaattttt
    13321 aaagaagctg atttgccacg aagatttata ccagcagctt tgacttttgg atgctcaact
    13381 tttgctatgg ttgcaccagg ggcaccccaa attcagaata ttgttccagc atcaacatta
    13441 ggtacagata ttatggctgg agcagtaaat ggttttatat catgtgcagt gatgtttata
    13501 gttggaagca ccattttgta taaaatggtt tcaaaagaaa aagccaatgg tggtcatttt
    13561 atagcacatg aatcagatat atttgatgaa gcagaagcaa ctaattctaa gagagaaaat
    13621 ggtcctaatg gtgtaattgc cttaattcca ttaatagtgt caattttaat aattaatgta
    13681 aaagttaatg gaaaagcgat agtaccaatt gaggtgggag tatttattgg agctttatta
    13741 gtgtatttat tgttaaataa atatcaagat aatactaaaa tagttggaca tgttggtgat
    13801 gcatgtaaaa ctacagtagt agcaatatgt aatacttgtg ctgtagttgc atttggttca
    13861 gtagtaaaat cagcagttgg ttttgatttc atagttaatg ccatgactaa tattcctggt
    13921 cctcctattg caggggctgc agttggaact acagttattg caggtatttg tgggtcagct
    13981 tcaggtggac taggaattgc agctccactt cttggaccag tatttttagc taagggtgtt
    14041 tctgctgcag cattaactag gactatggca atttcatcag cagcattaga ttcacttcca
    14101 cacaatggat atattgttac tgtaacaaat ggtctgtgta aagaaacgca taaggacgcc
    14161 tatatgccaa tattctgggt aactgtttta actccgttta ttggaaccat agtagcagtt
    14221 ctactgttta caatgttccc aggattacca taagagagaa gataaaaggt ataaattata
    14281 ttttgatatt ataaaatcaa ttttcaaaat atatacaata aatttcatgt tttaatactg
    14341 taaatatgta tttaaaataa caaattgacc atataaggag gaattgaaag tgttaaataa
    14401 accattagag ggagttaagg ttattgacct aacgtatttt gtagcaggtc cagggacttc
    14461 taaaatatta gctgactggg gcgctgacgt tattaaggta gagccaagct ttggagaccc
    14521 aggaagaaag acaggggcaa caatgacaat gccaatagat gattataata atccttttta
    14581 cagcacatat aattcaaata aaagaggttt gtctataaat cttaagagtg aaactggaat
    14641 tgaaattatg gataaacttt taagtgaagc aaacgtattt gtttcaagtt atagaacagg
    14701 agcgttaaaa agactaggtc ttgattatga atcattaagt aaaaagcatc cacaccttat
    14761 ctggggtcag ataaatggtt ttggcgattt tggacctgct aaggacaatg caggatttga
    14821 tacagttgcc ttttgggcaa gaagtggagc tatgcttgat atagcagaaa aagatacaag
    14881 cccaataaat ccgcctatag cttttggaga tgcaaccact tcttgtagtt tatctggagg
    14941 aatatgtgca gctttgtacc agcaagctaa gactggaaaa ggacaaaaag taatggcatc
    15001 tttatttggg caagcaatat ggaatgcatc atctttatta gcatctacac agtttagtga
    15061 tgagtatcca aaaactagaa aaaatgctat ttcaccagta ataaattctt atcgttgtaa
    15121 ggatggaaag tggatattct taagtatttt agagcatgaa agatattttg aggcactttg
    15181 tagattattt ggaagagaag acttaataaa agaagagaaa tttgcatctt cagtagaaag
    15241 taagaaaaat gctacagaac ttattaatat tttggatggt gaatttgcaa agtttactca
    15301 aaatgagatg gttgaaaaac ttactgctgc tgatatagct catgaaaaaa ttcaacatgt
    15361 taatgatgta gtgactgatg aacaggctat tgctaataac tatatatatg aacatacaag
    15421 taaaaatgga aataagacaa tgcttgcatc tactccagtt aaatttggaa atattgaagt
    15481 taacatgact tgtgatgctc cacttatagg agaacattca gatgagatat tgagagaact
    15541 tggatacagt gattctgata tagaggctct aattgaatct ggaattgtaa caatgaataa
    15601 agaagcatgt gaaatatgtt aataaacatg tgaagtatgt taataaatag gagggaattt
    15661 tatggatttt agattaacag aagcacaatt aatgcttcaa agagtagcaa aagaatttgc
    15721 agagaatgaa atagcaccaa tagctgctga aacagataaa acaggtattt ttccaagaga
    15781 gctttttagc aaaatggcta aaataggatt taatggaatt ggaacacctg tagagtatag
    15841 tggttctggt ggggcagata tagaaaaggt aattgttgtt actgagattg ccaaaaaatg
    15901 tgcagcatct gcagcaatat tatcaataca cacaatatat gctcaagcta ttttgaaatt
    15961 tggtactgag gaacaaaaga aaaaatacct tccaatgatg gctgaaggtg ggtgtgtagg
    16021 tgcttttgcc ttaactgaac ctaatgctgg ttcagatgct gcaagagctg caacaactgc
    16081 aattattgat gaagaaactg atgaatatgt tttaaatgga actaagtgtt ttatttctgg
    16141 tggaggtcaa gcagaatcat taataatatt tgcactgaca gacccatcaa agggaataaa
    16201 gggaatgtct gctatcattg tcgacaaagg tactccaggt ttttcaatag gaaaaattga
    16261 agaaaaaatg ggtattcatg gttcagaaac tgcagagtta atatttgata actgtagagt
    16321 tccaaagtct aacttattag gaaaagaagg caaaggattt aatatagcta tgacttgttt
    16381 agatggagca agaattggag ttggagctca agctgtagga attgctgaag gggctttaga
    16441 agaaagtatc aaatattcta aagaaagagt ccagtttggc aagccaattt cagctttgca
    16501 aggtattcaa tggtatatag cagatatggc gacaatggtg gaatcagcaa aattattagt
    16561 atattatgct gcagatttga aagctagagg agaaaaacac actaaagaag ctgctatggc
    16621 aaaatacaat gcgtcacgta ctgcaagaga ggttacaaat ttagcccttc aaatccatgg
    16681 tggatatgga tatatgaagg attatccatt agagagaatg tacagagatg ccaaaataac
    16741 agaaatttac gaaggtactt cagaaataca caaagttgtt atttcaagag cagtactagg
    16801 ataggaggaa aagaatgaat atagttgttt gcttaaagca agttccagat actaatgaag
    16861 ttaaaataaa taaagagaca ggtactctaa taagagatgg tgtaccaagt ataatcaatc
    16921 cagatgatag aaatgcttta gaagaagctc taaaaatgaa agatgaacta ggagcagtga
    16981 ttaaggttat aagcatggga cctcctcaag ctaaaagtgc tttaaaagaa gctttggcaa
    17041 tgggagctga tgaagcttat ttaatctcag atagagcttt tggtggttca gatacttggg
    17101 ctacatctac aattattgct gctgctatag agaaggttgg aaagtatgat gtaatattct
    17161 gcggaagaca agctatagat ggagatacag ctcaggttgg accagaggtt gcagaatttt
    17221 tagggatacc acaagttact tatgcgaaag aagtaaaggt tcaagatgac aaattactag
    17281 taactcgtta tacagagaca ggggattatt taatagaagc aaagatgcca gttttattaa
    17341 ctgctataaa agagttaaac aatccaagat atccaagtgt caaaggaatc ttagaagctt
    17401 ataataatgg agatgcaaag attactgtat taacacttgc agatttagat gttgatacaa
    17461 ctcaaattgg attaaagggt tcacctacaa acgtttataa gtcttttgta ccagttaaag
    17521 acaagcataa tgaaataata gaaggtataa ataaaaaaga aaaagctgag aaactaatag
    17581 aaatactatt cgatttgaaa ctagtatagg agtgttaaga tgatgagagc aaaagttaat
    17641 caaggtataa atttaaatga ttataatggt gtttgggtaa taggagagca aagggaaggt
    17701 aaaattaatc ctgttacgat agaacttatt ggtgaaggaa gaaagcttgc tgaccaatta
    17761 ggaaaagagc ttgcagttgt gatagctgga tatgaagtag agaaagaagt gaaggaatta
    17821 cttcactata gtgttgataa agtatattac atcaatgacc ctcttcttaa agatttcact
    17881 actgatggat atgcaatttc aattgcaaat ttaatcgaaa gaaaaaagcc agaggttgta
    17941 ttagttggtg ctacttctat aggaagagat atagcgccta gaattgcagg taaggttgga
    18001 acaggtctaa ctgctgactg tacaaaactt gaaatagatt caacagataa taagttatta
    18061 caaacaagac cagcatttgg tggaaattta atggccacta ttgtttgtcc aaaaaataga
    18121 ccacagatgt caacagttcg tccaggagtt atggcaaaag ctgtaagaaa cgaatcagag
    18181 actggaattt tagaagttgt tactccagaa cttactgaaa aaatgattag aactagatta
    18241 gtagagatac ttccacaaga aaagaaatct gtaaatctaa ctgatgctag aataatagta
    18301 tcagggggaa gaggattaaa aagagcagaa ggatttgagc ttataaaaga attggctgat
    18361 aaattggggg cagagattgg tgcttcaaga gcagcagtag attcagggtg gatagaacat
    18421 tctcatcaag tgggacaaac aggaactact gttagaccag aactatacat tgcatgtgga
    18481 atatctggag caatacaaca tttagcaggt atgagtgatt ctaaatatat cgttgctata
    18541 aataaagatg caaaagcccc tatttttagt atatgtgact atggaatagt aggagattta
    18601 tatgaaatta tacctgaaat gatagaatca ttaaataggt aacttctaga aaacattgaa
    18661 tatgtggaat gataagtctt ataatattgt attgtaagac ttattatttt tgctgaaggt
    18721 tatagtgggg agatgaaaca atgaaagagc agtggtataa ggatatattt gcaagagtat
    18781 tatctatgac tgatgatggt tttattgttg taaatacaag tggagtaata atagatatta
    18841 acgataaata ttgtgacttt ttaggaaaag aaagaaaaga tataatagga caaaatatac
    18901 aaagtattat accaaataca aaaatgctag acgttatgaa agataagtac tgtgaagaag
    18961 gagctataca tcattattca ggtggaaata caaaagaaaa aagtgtaata gtaagtaggt
    19021 cttatgtgga aaatgacaat ggagaagtag ttgcaggagt agctcaagtt aaatttagat
    19081 tgcaatcatt tgatgtggct aaaaagctaa tgtcagaata tatggagctc caatattata
    19141 aagagcagtt taaggataac tgtggttttg acaagctcat aggtgaaaat agagacttta
    19201 tagaacttaa gaaaacaggt gtgaaagctt ctaaaacaaa ctttccagta cttttaacgg
    19261 gtgaaacagg cacaggtaag gaagtctttg ctagagcaat tcacaataat agcagtaggt
    19321 cagacaaacc tatggttagt ataaactgcg ctgcaatacc agaagaatta ttagagtcag
    19381 agctatttgg ttatgacgaa ggagctttta caggagcaaa aaagggtggc aaaaaaggta
    19441 agtttttagt tgcgaataat ggtacaatct ttttagatga gataggtgac atgcctttga
    19501 ccatgcaagc aaaactttta agagttcttc aggaaagtga gatagaacca gtgggaggtc
    19561 ttaaaactat aaagatagat gtaagagtaa tctctgctac aagaaagaat ttaagtaaaa
    19621 tggttgaaga gggattattt agagaagacc tatactatag attaaatgta ataaacattc
    19681 atatgatgga attgaaggat aggcaagacg atattttatt gctagcaaat tatatattaa
    19741 acaaattaaa tgttgaatac aaggaattga aggttttaag tgataaggtt aaaaattgtt
    19801 ttattaacta tacttggcca ggaaatataa gagaactaca aaatgtaata aaaagtgcct
    19861 atgctgtaag tgatgatatg gtcattatga tgtgtgacct tccatctaag atggataata
    19921 tcagcagagt agctcaatgt aatgtggata gcaactgttc aattcatgaa atggttgaga
    19981 attatgaaaa gagcttaatt attgatgtgt taaggaagta taattggaaa tgttctaagg
    20041 cagcagaggt tatgggaata cacaagagtc ttttatataa gaaaataaaa aagtatgaaa
    20101 ttgaattaaa taattaagat aacttgaatt aaggcaatgc taaaaaaagc gcccttattc
    20161 aaagcgaact gtataaactg ttttatgcag ttaaaacgtg taaaagtaga tgggaagtac
    20221 tagtgtcaat cattagtact tttttattgt atttataatt actatttata tttcagtatc
    20281 ttttgatatt taagattatt atgtttaatt taggacataa caaaaccctc ttagttatgg
    20341 acagtttttt atataactgt ctactaagag gggatagttt agttcaattt tagagtaaat
    20401 tttatctagc atttataaaa aatattttga atagcttttt tgttatttaa tcataatttt
    20461 ctacttcttt atcaagttct ttgccacaag aatcacactt atatatagca ttgtatccgt
    20521 aatcatattc atcaactatt ttcttttgtc taaatacacc accacactca cataggaaac
    20581 caatttttat aagtgtaacg ataaattgtg attttataaa ttttatatca ttacatttat
    20641 cgttagtgta aaatgtataa atataagagt tgtcgcactc atctcttaat tcaaatcttt
    20701 caatatatgt gttatttttt aattcctcaa attcttcttt agttaaatat gatttttgtt
    20761 gcaattcatg taaacgctta atttggtcca ttatttatat cctcccaata attttagtat
    20821 tttaagctta gataaggaat ctaagtttta ttaaatttat accaaggaat tatttaaatc
    20881 atatttagtt ttattaatta caacatatat aaactacacg aaaataaaac taattgtaaa
    20941 aataatatcc tgtgaaaacc taaactacta accccttact aacccgaaaa ctaaaaaaag
    21001 aaaatatatc caaatataag tgatatataa ataaagatgt tttatataaa tttaacttta
    21061 aatattctga caataatata tataaatata ttatagttaa aatataccac atattttggg
    21121 tattgtatac tggaattaga gtaattaatt tattttttaa catacaaatg attctgtgaa
    21181 aaaaaacaag actcaaaaat ttttatattc tttgatagaa taaaattgag agtaaattta
    21241 ttgaatttta taaaatttat aaggggtgag tctaagatat gcatgaagat aaaaatgata
    21301 tatttgaatc aatagatact gaggattttg ataataaaac agaagaagaa tgtttagagc
    21361 tagtaagaaa aaatggattg aatttaatat acataaaaaa tcaaacagaa gatatatgtc
    21421 tagaggctgt tagacaaaat tgtaatgcaa taaaatgtgt tcagaatcag acagaaaaaa
    21481 tatgtataga agcagttaaa caagattgga gaatgttaga atttgtggaa gaaccaacag
    21541 aagaaatttg catgttggct attaatcaag atggaacagc attaaaatat atagaaaatc
    21601 agacagagga gttatgctta agagctgttg aaaaaaatgg tgctgctttg gagtatgtaa
    21661 aagaacaaac agaagaaata tgtatagaag cagttagaaa cagcgaattt ggattggcaa
    21721 gagtgaaaaa tcaaacagaa aaaatatgta tggaagcagt taagcattgt agctataatc
    21781 taaaatgggt agaaaatcaa acagaagaaa tatgtatgga agcagtgaga caaaatgggc
    21841 ttgatttgaa atttgtaaaa aatcaaactg aaacaatatg tttaagagca gtaagacaaa
    21901 atggaatggc attagaattt gtaaaggaac agactgttgg tatatgttta aaggcagtaa
    21961 gacaatatgg aatggcatta aaatttgtaa aagaacaaac agaagaaatt tgtacagaag
    22021 ctataaaaca agataaaaga gcacttagtt ttgtaaaagg agataaggaa aaatacaagg
    22081 ctctatatga taataatgaa ccatttgcaa agcgatatgt tagaaatgta atagaaaaag
    22141 aaaacagagc attgattaaa aaaggagaag aaaatgcaaa aatcaaaatt gcaggaaaac
    22201 tttatgatag aggcatgtct tttgaagata tatcagatat agttgagatt gaaataagca
    22261 agttaaaagt gagtttaggt attttttaaa gtaataaatg tagacgtttt ttatgggaaa
    22321 tagttaataa aaaaataatc gactagctct ttgtaggaca gcaaaattag tgatataatt
    22381 tgatgtataa taaattattt ttattagata agggggaatt aaagttgaag attacagtat
    22441 tataccaaac aagaagtgga aacacagaaa gggttgctaa gctgatagaa gatggagcta
    22501 aaaaggtgga tggagtagat gtaaagctta tgagacttga taatattgat ttagattatt
    22561 taaatgaaag taaagcagta atatttggaa cacctactta tttagcaaat acaacttggg
    22621 aagtaaaaaa atggtttgat gaagattcaa aaaaagttaa tttagctgga aaattaggag
    22681 ctgtttttgc tacatgtgat tatatatgtg gaggtccaga tgttgcgata cttactatag
    22741 taggacattt gatggttaaa gggatgcttg tgtattctgg aggtggttct ttaggaaagc
    22801 catttataca tttaggacat gtacattcaa ttgaaggtcc agagttacaa gatgagaagg
    22861 ctataatatt tggtgaaaga attgctacta aggcaaaaga actatttgct taatttctat
    22921 atgcataata atgattagag taaatttagg gttttaatgg agatttgaag taaaagttat
    22981 aaaataaaaa attttccata aaattactca agatgaatta taatataatt ataaataaat
    23041 tatattatta aaatatttaa aatcaaacgt caatagcgag ggaagtttat ggtatggcat
    23101 atagaatatg agattttttc tgcaattata gttatttttt taatggtata cttttttaga
    23161 agtaaattta tacctacttt acaaaacaaa atatattgtg cacttttaat attctcattt
    23221 ttatttataa tttcaaatat tttaggttca ttttgtctaa ataatataga caaaattcca
    23281 atttttatta cttttttatt gaatcagata tacttattgt tattgccaat accagcagca
    23341 cttgtatcat tttatgttat ggctattatt tatcaagata taaggtatat gaaaaaaaga
    23401 ttattatttt tatctatacc acttataatt tgtataatct tatctattac aaatcctttt
    23461 acgcatattc tatttggatt gtcaaaagaa acaggttata tacgaggttg tggatatgtt
    23521 gcaacttttg catctttttt cttttatgcg atatattcag catttttatc ttttaagtat
    23581 agaaaagcaa tgcatgaatc aaaaatatgg gctattagag gttttcttat agtttcaatt
    23641 gtggcaatta tattacaggc aattttttta cagtatcttc ttacaggaac tgcatgtgca
    23701 tgtagtttac ttttgacata tctttctatg caaaatcgag gtcttattat tgatgacctt
    23761 actggtgtac tcaatagaca atcttttatt caagagctag atttgaatat caatactgaa
    23821 caatcaggat ttattattac aattgctctt gatgatttta agtttatgaa tgaaactttt
    23881 ggaacaaaaa atggagatat tgcactaaaa gaagttggaa aatatttgat tcagatacat
    23941 gatgtaaacc atgtatatag aataggggga gatatatttt ctattgttgt caatagtcaa
    24001 ttaggtatag aaccagatga tattatcaga aagatagaag aaagatttaa aaagccttgg
    24061 ctagtagaag agataagttt taatttagca actagtattg ctgttgttta ttacccagaa
    24121 aatgcagaaa cgacagaaga tgtaattgtt gctatagatt tctctataca tgaagcaaaa
    24181 cgttctgagt caaggcgagt tgtttatgca gatgcgtcta taagtgaaaa gataaagaag
    24241 aaacatgtta ttaaaaattg tttaaaagaa gcattaaaaa atgatgggtt tgaagtgtac
    24301 taccaaccta tattttctaa taacaggggc aggtttactt cagcagaggc attacttagg
    24361 ctggaacata aagagttagg ttttatacct cctgatgaat ttattccaat tgcagaacaa
    24421 acaggattga ttaactcaat tggattagta gtctttgaga aggtgtgtag atttatagct
    24481 agtgatgagt ttgaaaattt aaaattggat aatatagctg tgaatctttc tgtagtacaa
    24541 tgtatgcaaa aaaacttagc tgatgattta ctttatctta tgaataaata taatgtatca
    24601 ccaactaaat ttaaacttga aattactgag actgtggcta tgggctcttt taatataatt
    24661 aaagaaacaa tggaaagatt aattgattta ggtgttaagt ttgcacttga tgattttgga
    24721 attgcttatt ctggagttac taatatgctt tctttaccat tttcagtaat taagttagat
    24781 aagagtttga tttggtctat gaatgaagat agtaggcata aacttacagt tgagacgatt
    24841 atagctctta taaataaatt gaatatgaag gctgtagcag aaggcgttga aaccattaag
    24901 tgtgctgaag atttaatagc tatggagtgt gaatatttgc aagggtatta tttttctaaa
    24961 ccagttccag aagatgtttt taaaaatttg ttgaaagaaa agacttttac aagttagcac
    25021 cctttatttt tttaattaca actttgttca ttgacactgt cagcgatata ttataaaatt
    25081 aacacaataa taagtattaa atttattata catattatta atattaatta atggagtaaa
    25141 gatttattat atatttttat aacttagaat atatatttta tttaattatc ctaaagtgag
    25201 aaaggaagat gtgacatgaa taaaaagaaa atattagtgc caattgatgg gacagaaaga
    25261 agtatgcatt ctttggaatt tataaaagga atattcaaaa aagatgaagt tgaagtagaa
    25321 ataatgaatg taaaagaact agtatttatt gatggcatat ccctggctga agagataaaa
    25381 aattctgaaa acttaggaag aagaatcttg gataaagcag cagagataat gggagagtat
    25441 gatgttaaag tacattttac ttttggatat ccaggagatg agataattag aaaagcaaaa
    25501 gaagataatg tagattttat agttatgaca aaatctacta aaaaaggtct tacaagaatg
    25561 ataggttcag taacagcaag tgtagttaaa caagcaaaat gtatagtgat gattgtaccg
    25621 gagtagaatt agaaaggtat ttgaaatatg aataaaaaga tagtagcagt ttgtgaaagt
    25681 gaaagaagtt taaattctct taagacagct gcaaatgatt taggatttga tttagtttat
    25741 gaaatccaag aaaatgataa tataaaaaat gaactttcta tttatgatat agaagatgca
    25801 tctatagtgt tatttgttac aagtgattct atagaaagca tagagaagat tgatagattt
    25861 atagatagag aattttatga agttgatcca aagtatataa tagaggatgc aaaatctata
    25921 gtaagtgaaa ttttaataga cttaaattaa tcatgggtga ctcaaactgt gccttaaaat
    25981 gaacttttta gttcatttta aggcactttt tttctatgaa atcaaatata tttttattgt
    26041 ttcaacaatg tgattatttc tatttttaca caagtctttg agataattta tttttgtatg
    26101 atagttgtat ttaaaggaaa aagaagttta atcatcatgc tgaaatagct gaataacatc
    26161 ttctaaatga ttgtacattg atgtataacc taagttggag tctttactat caaaggctat
    26221 tattatgtta ttatgatggc taatcatatc ttttagagaa tttctgtatt taaaaccaaa
    26281 gtattgatta gagaaaattg ttttaggtat taactcataa tactttataa aaattgggtt
    26341 atgagaggtt aaaattatct ctttgtgaaa ttgcaagtca gcatcataac attcttctat
    26401 caaaccatga gaaaccttat ccaattcctc tattgacttt aggtaagtat attttatttt
    26461 ttgaagttct ttagatgtag catttaaaat tgctagtcta gtagcttcac tttcgataag
    26521 ttgtcttgct cttaaagatt ctacataaaa ttcttgtatg ttatgatttt tatagatttc
    26581 accatcatca aataggtcaa cttttttaaa tccttcaact ataggacttg aataaggcac
    26641 agctctaagt attccttctt tacagagtat atttatggca tttcttacag gtattctact
    26701 tatgttaaat aggtcagcaa tctttctctc acttggcaat ttactattaa ttttaaaagt
    26761 tccatttaga atgtttaatt taatataatc aataattttt tcagtggcat ttaagctttt
    26821 cataaaaact cctaaacata taattttttt atgattgtat tatatcatag ttaagagtta
    26881 aatctacttt tttataatat gtataagaca ggatagaggc caccatagct ataatggcca
    26941 agataaagta tccatattgt attgaagtat attccgatat tttaccaact atcattggaa
    27001 acacagacat tcctatagca taaattgctt gaaaaaaacc cattgcagta gatttctgat
    27061 gttttggtac atttttcata gactcgctag taaggtaaga aaggagtata cctgtagata
    27121 tacctggtag cacttgtagt acaaatatta tattaatatt ttttattgtt ggaactaaaa
    27181 agcaataagc tgaaactaaa ataaaaacta aaggaatcca aaattttggt ccatatttaa
    27241 tacaaaattt agtagaagca aagctagaag aggcaacagc acacaccatg taaaatatag
    27301 aagatagacc tataagggta gaactagaac ctaagttttt aagaatttga gtagtaaatg
    27361 acattgttgt agacatttga ataccctgtt gaattaatgc cataaaggaa aaaaatataa
    27421 gtcttttatc cttgcagata gataaatact ttttaattga tattttttca ggcatttttt
    27481 taaaattata attttttata tttaaggcta gaatagttcc aataactgaa gacaatacac
    27541 tcatgataca taacgctgtc attcctaatt tttcataaaa taaagtactc ataacaaatc
    27601 caatcaacat ccctagattg tttactaata ttatcgtact tgtagctttt tgttgatgtt
    27661 ccatagaaaa aaagttggta tagtgaacca tgtaagaaat ccaagttgat gatgatattc
    27721 cagataaaat gttagctata agaaaaccat tgcctccagg agaaaagact cttataagag
    27781 atgctaaacc agcaaagaaa attccagaca gtataaatat tttatgttta tttatggaat
    27841 cagcaaatac tccaacagga agccttagta aacattgcga gattccatat gcacctatta
    27901 ttataccaat catatttgta gatacattgc atgcacttaa atatggattt tggaaaggta
    27961 tatatacata ttgtgaaaac cagaaaaatg taacaataga aagtaaaagt aatttttgat
    28021 tagatatttt
//
LOCUS       DAEMAE010000025        27801 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEMAE010000025 DAEMAE010000000
VERSION     DAEMAE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.025"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            94..969
                     /locus_tag="KPH93_000904"
     CDS             94..969
                     /locus_tag="KPH93_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG1687738.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKCIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDKVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            987..2477
                     /gene="cls"
                     /locus_tag="KPH93_000905"
     CDS             987..2477
                     /gene="cls"
                     /locus_tag="KPH93_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG1687739.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2670..2954
                     /locus_tag="KPH93_000906"
     CDS             2670..2954
                     /locus_tag="KPH93_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG1687740.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            2987..4615
                     /gene="groL"
                     /locus_tag="KPH93_000907"
     CDS             2987..4615
                     /gene="groL"
                     /locus_tag="KPH93_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG1687741.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKSIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4877..5617
                     /gene="larB"
                     /locus_tag="KPH93_000908"
     CDS             4877..5617
                     /gene="larB"
                     /locus_tag="KPH93_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG1687742.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDIGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEESSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDGARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLAAMINKL"
     gene            5701..6435
                     /gene="larC"
                     /locus_tag="KPH93_000909"
     CDS             5701..6435
                     /gene="larC"
                     /locus_tag="KPH93_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG1687743.1"
                     /translation="MSEKILYFDIINGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGVIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKILCDEFVDEFEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6465..6884
                     /locus_tag="KPH93_000910"
     CDS             6465..6884
                     /locus_tag="KPH93_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG1687744.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGELVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6944..7045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7266..8801
                     /gene="guaA"
                     /locus_tag="KPH93_000911"
     CDS             7266..8801
                     /gene="guaA"
                     /locus_tag="KPH93_000911"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG1687745.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9317..10747
                     /locus_tag="KPH93_000912"
     CDS             9317..10747
                     /locus_tag="KPH93_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1687746.1"
                     /translation="MTLNQIFIEDGGVMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKIDILSANTY
                     KKNGDSYTKPYIIKNINTPEGNIKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10895..11770)
                     /locus_tag="KPH93_000913"
     CDS             complement(10895..11770)
                     /locus_tag="KPH93_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1687747.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCMFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIRAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            11890..12453
                     /locus_tag="KPH93_000914"
     CDS             11890..12453
                     /locus_tag="KPH93_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1687748.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDLCSMILKQRSLFLSVMIPIAFFANFVM
                     SINVIFILSVCCCVCVMQVFYKKRENK"
     gene            12450..13016
                     /locus_tag="KPH93_000915"
     CDS             12450..13016
                     /locus_tag="KPH93_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1687749.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13145..13381)
                     /locus_tag="KPH93_000916"
     CDS             complement(13145..13381)
                     /locus_tag="KPH93_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687750.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13691..17119)
                     /gene="uvrA"
                     /locus_tag="KPH93_000917"
     CDS             complement(13691..17119)
                     /gene="uvrA"
                     /locus_tag="KPH93_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1687751.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPVELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTVPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIKIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWPSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSFCHNKNSIYLKGGRQN
                     NLKDIEVTIPKNKITVITGVSGSGKSSLAFDTIYEEGKRCYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESRGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQQNELSLVNDILTELKIRISYLLK
                     VGLSYLSTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKIISQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTKLTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTITDILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEIIRVSDWIIDIGPDSGINGGKIVASGTPDEIKINPNSITGHFI"
     gene            17438..19672
                     /locus_tag="KPH93_000918"
     CDS             17438..19672
                     /locus_tag="KPH93_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1687752.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDIGI
                     IDLEGKAEFLSGKKFDLKNTDSYKKGIKGKEYSEIIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSDKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKGSGDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHSITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTIIMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMVAINIKDFKLINDCFGSEKGNMTLKHLYNVLYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRISVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKLICNKYNVPTYMIDLELTESIFLENPEAVD
                     VIDDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIYY"
     gene            19930..21960
                     /locus_tag="KPH93_000919"
     CDS             19930..21960
                     /locus_tag="KPH93_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1687753.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKAFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQRNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEEMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFRDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHRYEKEVTV"
     gene            21957..22409
                     /locus_tag="KPH93_000920"
     CDS             21957..22409
                     /locus_tag="KPH93_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1687754.1"
                     /translation="MSKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            22411..23466
                     /locus_tag="KPH93_000921"
     CDS             22411..23466
                     /locus_tag="KPH93_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG1687755.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDDSIELIFEEL"
     gene            23533..23841
                     /locus_tag="KPH93_000922"
     CDS             23533..23841
                     /locus_tag="KPH93_000922"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG1687756.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSDIIKKSDALIKKIKDTFQ
                     "
     gene            23945..25210
                     /locus_tag="KPH93_000923"
     CDS             23945..25210
                     /locus_tag="KPH93_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG1687757.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKIIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSIETYETFITRLKVELDKRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVIGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYANLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTARILNK"
     gene            25298..26014
                     /locus_tag="KPH93_000924"
     CDS             25298..26014
                     /locus_tag="KPH93_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG1687758.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDA
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEDLFYKPDFV
                     ILEYKDLMSLFIKICSYKNM"
     gene            complement(26209..26502)
                     /locus_tag="KPH93_000925"
     CDS             complement(26209..26502)
                     /locus_tag="KPH93_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG1687759.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(26508..26723)
                     /locus_tag="KPH93_000926"
     CDS             complement(26508..26723)
                     /locus_tag="KPH93_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687760.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(27267..27779)
                     /locus_tag="KPH93_000927"
     CDS             complement(27267..27779)
                     /locus_tag="KPH93_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1687761.1"
                     /translation="MLEVIKNRRSIRTYKDKNIEEEKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHVEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDKN
                     KIHRNVYKNR"
BASE COUNT        10438 a         3118 c         4787 g         9458 t
ORIGIN      
        1 ctttgtgtta aaataagtaa aatgcacata ttagagctaa gtttttaagc aaataaacaa
       61 aatgaaaata taaatattaa ggagatttgg tatatgaatg tttatgaaaa aggtaatgga
      121 gttatactag aaggagtaac agattttgac ccagtacata tatttgagtg tggtcaatgt
      181 tttagatggc acaaacaaga agatggctca tacactggag ttgcaaaggg gagaatatta
      241 aatgtcaaaa aagagaatga taaaatatat ttaaataata ctaatttaaa ggagtttaac
      301 agcatttggt ataattactt tgatttagga actgattata cagaaataaa gaataagcta
      361 aaaaatatgg atgaatattt aaataaggct actgaatttg gatggggaat aagaatatta
      421 agacaagatg gatgggaaat gcttatatca tttataattt cctctaataa taggattcct
      481 atgatacaaa gagctataga aaatttatca agaaaatttg gtaagtgtat aggtgaatat
      541 gaaggtaatg aatactatgc attcccaaca ccagaagaac taaataaggc ctcacaagag
      601 gaaataagag catgtcaaac aggttttaga gataagtaca taaaaagtac tacacaggca
      661 gttatagaga ataatgataa agtatctgaa tatacaaatt taagtacaga ggattgtaga
      721 aaagagcttt taaaatttaa tggagtagga ccaaaagtat gtgattgtat tgcattattt
      781 ggtatgcaaa aatatgattc ttttccagtt gatgtttggg ttaagagagt tatgcaagaa
      841 ttttatatag atgaagacat gagtttacct aaaatgagaa cttatggaat cgataaattt
      901 aaagaaatgt ctggatttgc tcaacagtac ttattttact atgcaagaga actcggtata
      961 ggaaaataaa ttaaggggga gaaattatgg gagtaatagg aacaatattt ctgttctatt
     1021 taattatatc atatctagct ggagccataa tttctgtgat tatattactt gaaaatagag
     1081 accctgcaaa aacaatgtct tggttattga tgtttattat atttccaggt gtaggtttaa
     1141 tgatatatgc tatttcaggt agaaatatta gaaaaagaaa attatttaag actcaaaaac
     1201 tagctaataa tataaaagaa aagaaactat ttgatacatt agaaaagata acagaaatag
     1261 ttgaattaga aaaagaatct ataaaacaaa ataagctttt aagagatgaa gaggatggta
     1321 gttatagaaa aagggtaata aatatgttat taaagactgg tatgtttccc tttactaaaa
     1381 ataataaagt ggatgtgttt gtagatggaa atgaaaagtt taaaagactt atagaagata
     1441 taagagaagc taaagaccat attcatttag agtactttat catcaaagat agtgaaatag
     1501 gtagggtgtt aaaagaagaa cttataaaaa aggctaaaga aggcattaag ataagaattt
     1561 tatatgatga tgtaggatgc tggagatttt ggtttaatag aaaatttttc cgagaaatga
     1621 gggaagtagg tatagaaatt gcagcatttt tacctacaaa atttcctata ataggaggga
     1681 aattaaacta tagaaatcac agaaaaattg ttgttattga tggaataatt ggatatacag
     1741 gtggaataaa tataggagat gaatatttag gcaaaaatga taagtttgga tattggagag
     1801 atactcatat taggattaag ggtatttctg tatacatgct tcaaatgaca tttctgattg
     1861 attggtatta tacgacaaaa gaagtattag taactaaaaa ttattttcca agtgtaggga
     1921 atattggtga aagtatgata caagttgttg cgagtggtcc agacagtgat tgggaagata
     1981 ttcattatgc ttatttttct gcaatatgtc aggctagaaa aaatgtttat atagaaactc
     2041 catattttat tccagatgaa agtctattaa aagctataaa gagtgctgct cttagtggag
     2101 ttgatgtgag aataatattt cctaaaattg cagaccacaa gatagtaaat atagcatctt
     2161 attcatattt tgaagaaata ttgagagcag gtggtaaagt ttatttatat aataaaggat
     2221 ttatacattc taaagtcgta ataatagatg ataaaattgc ttctgcaggt acagctaata
     2281 tggatttaag aagttttatg cttaactttg aagtaaatgc atttatctat gatgaagaag
     2341 ttattagggt aatgacagat gatttctttg aggatttaag tcactgtgaa gaacttaatt
     2401 tggaggtatt taagaataga aatatcatac aaaaaatcaa agaatctgta gcaagattat
     2461 tttcaccaat attataaaaa attattaaaa aaggtattga aaattatgtg tgattggtat
     2521 attataataa ttgttagcac tctattaggt agagtgataa taaataaaaa gtatatatat
     2581 aaatctacat atataagtag tattaattta aaaataacta aaaacatagg cgaactagtc
     2641 tatacttaat attttaggag gcgtgaataa tgaaaataag accattagct gacagagtag
     2701 taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg aatagtttta ccaggagcag
     2761 ctaaagagca acctcaaata gctgaagttg tagaagttgg accaggtgga atagttgaag
     2821 ggaaagaaat aaaaatggaa ttgacagtag gagataaggt tatattccaa aaatattctg
     2881 gaacagaagt taagatagaa ggacaagaat atacaatact aagacagagt gatgtattag
     2941 ctgttattga ataaatatag aataaattta ttaggagggg tttaaaatgg ctaaagaaat
     3001 taaattttca gaggaaacaa gaagagcatt agaagctggt gtaaataaat tagcagatac
     3061 agtaaaagta acattaggac ctaaaggaag aaatgttata ttagataaaa aatttggttc
     3121 tccacttata actaatgatg gagtaactat agcaaaagag atagagttag aagatagatt
     3181 tgaaaatatg ggtgctcaat tagttaaaga agtagcaact aaaactaatg atgtagctgg
     3241 agatggtact acaactgcta cagttttagc acaagctata ataagagaag gtttaaagaa
     3301 tgtaacagca ggggctaacc caatactttt aagaaaagga atacaaaaag cagtaacagt
     3361 agcagtagaa gaattaaaaa atcaatcaag aatagtagaa acacaagagg ctatatctca
     3421 agttgcttct atatctgctg gtgatgaaga agttggaaaa ttaatagcag aagctatgga
     3481 aatagtaggt aaagatggag ttataactgt tgaagagtct caaactatga atactgagtt
     3541 agatgctgtt gaaggtatgc agtttgatag aggttttgtt tctgcatata tggttacaga
     3601 tgtagataaa atggaagcag ttttaaatga cccatatata ttaattactg ataaaaaaat
     3661 atctaacata caagaattat taccagttct tgaacaaata gttcaacaag gtaaaaagct
     3721 attgataata gctgaagatg tagaaggtga agcattatct acattagtag taaataaatt
     3781 aagaggaaca tttgatgtag tagcagttaa agctccaggc tttggagata gaagaaaaga
     3841 aatgcttcaa gatatagcaa tacttacagg tgctcaagta atatcagaag aattaggtta
     3901 tgatttaaaa gaagctgatt tatctatgtt aggtagagct tcatctgtta aggtaactaa
     3961 agaaagtact acaatagtag atggttctgg agataaaaaa tctatagaag atagagtaac
     4021 tcaaattaaa catcaagtag agcaaactac ttcagatttt gatagagaaa aattaatgga
     4081 aagattagct aaacttgctg gaggagtagc tgttgtaaaa gttggagctg ctacagaagt
     4141 agagttaaaa gaaagaaagt taagaataga agatgccctt aatgcaacta gagcagctgt
     4201 agaagaagga atagtagctg gtggaggaac tgcttttgtt agtgttatac cagcaatagg
     4261 aacactaatt gagagtttag aaggagaagt taaactaggt gctcaaatag ttaaaaaagc
     4321 gttagaagag ccattaagac aaatagctat aaatgctggt cttgaaggtg ctgtaattgt
     4381 gcaaaatgtt gttaattctg aagcagaaac tggatttgac gcattgaatg aaaaatatgt
     4441 aaatatgata gaagctggta tagttgaccc aactaaagtt agtagaagtg ctttacaaaa
     4501 tgctgcatca atagcaagta ctttcttaac tactgaagct gctgttgctg accttcctga
     4561 aaaagaagat gcaggaatgc caggaatggg tggcggaatg ccaggtatga tgtaatttaa
     4621 aaaatttaaa atataaattt taatataaag aactatttcg acttagattt taaactctaa
     4681 gttgaaatag ttcttttgta tatatctaat ttttgtatat ttataaattc aatacacata
     4741 tttagataat cagtatctag tttgtaataa tattttagag tagtattgtt attttttagg
     4801 ttatgatttt agatatatat taattaaggt ataattgttg atagagaata aatcaaattt
     4861 aaaaggatgc gataatatgg acttaagaaa attgcttgaa gaagttaaaa atgaaaatat
     4921 tgatatagat tttgcgttag aaaaattaaa agatttacct tatgaagaca ttggatatgc
     4981 aaatatagac catcatagag aattaagaaa tgggtatccg gaagttattt attgtgaagg
     5041 taagagtgat gagcatattt tagggattat tgataaaatg aatcaaaaaa gttctaacat
     5101 attggggaca aggtgtagaa aagaaacttt tctaaaaatt aaagaaatct ataatcatgc
     5161 tgagtacgaa gagtcgtcta aaattcttaa gattcaaaat catgatatag agaatatagg
     5221 aaaaggtaaa attgtgattg ctacaggggg aacttctgat ataccagtag cagacgaagc
     5281 ctaccataca gctaagtttt taggcaatga tgttgataga atatatgatg ttggagtggc
     5341 aggtatacat aggcttttaa ataaaagaca taaaatagat ggtgctagag taatagtagc
     5401 tgttgcaggt atggaaggtg ctcttgcaag tgtaattggt ggactggttg atgttccagt
     5461 aatagctgtt ccaacttctg ttggatatgg agcaaatttt ggaggattag cagccttact
     5521 agctatgtta aatagctgtg catctggaat atctgttgta aatattgata atggatttgg
     5581 agcaggatat ttagcagcaa tgataaataa attgtaaata cattgatata aattaaacaa
     5641 aactatataa taaaatttaa tttctattat atttttacta ttaaaattga ggagacatat
     5701 atgagtgaaa aaatacttta ttttgatata ataaacggta tatcagggga tatgaccctt
     5761 gcaagtttac taaatttagg tgtacctaag gaaatttttt tggaggaaat tcataaacta
     5821 agaatgagcg atgaatttaa tataaatatc agctcgaaaa ctgaaaatgg aatagtaggt
     5881 acaaaagtag aagtaattac aaaagaaaaa catacacata gaaatttagt ggatatattt
     5941 gaggttatag ataaaagcac tctaaacgaa aatgtaaaat ctaaagcaaa aaaaatattt
     6001 atggtaattg gagaagcaga agcaaaagtt catggaacta caatagacaa gatacatttt
     6061 catgaggtag gggctataga ttcaattgta gatatagtag gagcttctat attagttgac
     6121 ttactttgtg tagataaagt ttgcgctagt acggtgtctg ttggttcagg atttgtaaaa
     6181 tgtgaacatg gagttatacc tatacctgca cctgcaacta tagaaatttt aaaaggtgtg
     6241 ccaattaaat taaacaatgt aaatggagaa tgtacaactc caacaggagc tgcaatcata
     6301 aaaattttgt gtgatgagtt tgtagatgag tttgaatttt ctccaaagca aattggatat
     6361 ggtataggtc ataagaagtt tgaagtgcca aatatactca gaactgtatt aggtgaaaaa
     6421 aaaaagaaga attaatttat gaaattagtg caaacattga tgatatgtct tcagaaatct
     6481 attcttattt gtatgaaaaa atattagatg agaaagcatt agatatttat acagaaagta
     6541 tttatatgaa gaagaataga ccagcaacaa aagtatcaat tttatgtaat gaatcagatt
     6601 tagaaaaatt tattaaaata ttacttttag aaactagtac ttttggagtg agatataatg
     6661 catataacag agaaattcta gagagaaaat ttgtaaaatt agatacaaaa tatggagtgg
     6721 ttacagtaaa attagggtat tataaaggag aactagtcaa ggcgactcca gaatataatg
     6781 aatgtaaaac tatagcaaaa aatgaagata ttcctataaa aaaagtgttt gctgatataa
     6841 attttagaat aaaagaagaa tttagtgaaa acttattgac ataagtaata aattttgata
     6901 aattaatatt cagccttatt aaataaaagt aaacagaaaa aaatatataa aatataaact
     6961 cactcgtata tgctcagaaa tatggtctga gagtctctac caagataccg taaatatctt
     7021 gactatgagt gaaattatta taccaaaaga cctagttaag taaatgtgta ggctgagttg
     7081 tactccctat gtgttggcta taagataaag gtattttgga agcctgtata gtaatttcac
     7141 ttttgctaga aattatggaa gttactatac aggttttttg tgtaaaaaga tttaatataa
     7201 tttagactgt attgtgttaa aataaatata taaaaactat attatacaag gagagatgtg
     7261 aatttatgaa acatgaatta gtacttgtaa tagattttgg agggcaatat aatcagttga
     7321 tagctagaag agttagagaa aacaacgtat attgtgaaat acttccatgt actgcaagca
     7381 tagaaagaat aaaagaaaaa aatcctaaag ggataatatt tacaggtggt ccaaatagtg
     7441 cgtatttaga agattctcca acaatatcaa aagaaatatt tgaactagga gtaccaatac
     7501 ttggaatatg ctatggtatt caaataatgt ctcatgtatt gggtggagtt gtaagaaaag
     7561 gtaataaaca agaaaaagaa tatggaaaaa cagctataac ttatggaaaa tcatcattat
     7621 ttgaaggaat aactacaaat agtgtatgga tgagtcatac agatttaata gaaaaagttc
     7681 cagaaggatt tactatagtt gcaaatacta atgattgtcc agtagcagct atggaaaatg
     7741 tagaacgaaa cttatatgga gttcaattcc atccagaggt agaacactgt ttggaagggg
     7801 acaagatact tacaaacttc ttatataata tttgtaaagt aaaaggtgac tggactacag
     7861 attcattcat agaagataaa ataaaagaat taaaagaaaa aataggcgat aaaaaagctt
     7921 tatgtgcttt aagtggagga gttgattctt ctgttgctgc agttcttatt cataaagcaa
     7981 ttggggacaa tttaacttgt atatttgttg accatggttt acttagaaaa aatgaaggaa
     8041 atgatgtaga aagaatattt agagagaaat ttgatataaa tttaataaga gttaatgcag
     8101 aagatagatt cttatctaaa ttaaaaggag tttctgaacc agaagctaag agaaaaataa
     8161 taggtgaaga atttataaga gtatttgaag aagaatcaaa taaattagga aaaatggatt
     8221 tcttagtaca aggaacaatc tatccagatg ttatagaaag tggacatgga aatgctgcta
     8281 ctataaagtc tcatcataat gttggtggaa tcccagaaga tgtagacttc caagaaattg
     8341 tagaaccatt aagagagtta tttaaggatg aagttagaaa gataggatta gagcttggta
     8401 tagaagaagg tttaatattt agacatcctt tcccaggacc aggtctagga ataagagtta
     8461 taggtgatgt aactaaagaa aaatgtgata tattaagaga agctgatgct gtttatatgg
     8521 acgagcttag aaaagcaggt ttatatagag aaatatggca agcttttgca acattaccag
     8581 atgttaagac tgttggagtt atgggtgatg aaagaacata tgcttattta gttggtttaa
     8641 gagcagttac ttcttctgat ggaatgactt ctgactggta taagatgcct tatgatgtat
     8701 tagaaagaat ttctaataga ataataaatg aagttgatgg agtaaataga gtagtttatg
     8761 atataacttc taaacctcct ggaactattg aatgggaata aagtatagaa tgccaaaaat
     8821 ccacatgaat acaggatttg aggtctctct tacttctata ttatactggt ttgatactat
     8881 caatatttgc cagtatctta tggattaata agcaaaaaac atcctgctga atgataaatt
     8941 cagcggggtg tttttatttt gttttggaac tcttttgctc tgaaactcga agtatttaac
     9001 actgatgatg ttgttatctt ttacttcctt ttgtgtcatg ctctgaaaga caaattgata
     9061 caataaaaaa gtaatttctt aatcattgat ggatatatgt atttatgtta cttttgatgg
     9121 taatttgagt aatattgttc atagcttttt atattcactt aattaaataa acttgatgat
     9181 ataatataaa agatttataa ttattagaag tatataaaag tattttatgt ctatgaaatg
     9241 tatgtctata aaacatataa atgacttttg tatattaatg tatgaatatt gatattatag
     9301 aagaattgag ttgtcaatga cactaaatca aatatttata gaagatggag gggttatgag
     9361 aaataaaata attataggaa ttactttttc acttgtatta ctaatgtctg gatgtgcttc
     9421 aaaacaaaca gcagaaataa acgtattagc aactactgat ttacatggag ttatacctta
     9481 tgagttaact tcttatgtaa aagaagaaag aaaaaaagat aaaaatctaa ctttagtaga
     9541 tgcaggagat ttttttgata gtggagaagt gtttggttct agcatggata gttattttag
     9601 tgctagatat aaagatggtg ataataacac tgaaagttat attgagacgc ctatttcaaa
     9661 agatatgaaa gaggtaggtt atgatgttgc agttcttggg aatcatgaat ttatatccaa
     9721 caataaattt tatctagata atatgatatc tgattttgag aagcaaaaaa tagatatttt
     9781 atcagcaaat acgtataaaa aaaatggaga tagttataca aaaccataca taataaaaaa
     9841 tattaataca cctgaaggaa acataaaact aggaatttta gggcttacta taaaagaagt
     9901 tggtgagagg aaacagtgga aagatggaaa acttgttgac tctaagtctt tagaactaaa
     9961 agaccaagag ggctacaatg gtgaattgta tatgaatgat ttagtagaag atgctaaaaa
    10021 gtgggttagt ataatggaaa aagagaagcc tgacattata gttgctgtgg cacatacagg
    10081 tgagaaacct aagaaaccta gaaacccagg aaatagaata caagacttag ctcaacaagt
    10141 tgatggaatt gatgctatag ttgcaggtca taatcatgtt caaattgagc aacatgatta
    10201 taaaaataaa tcaggagaaa atgttatagt tactgagcca ggaaagcatg gagaatgtat
    10261 ttctaagatt aattttaaat tagaaaaaaa caacgataaa tggaatatag ttgacaaatc
    10321 tagtaaatta attaaatttg aaaaaaatca agaaagtgat aaatttggag aatttatttc
    10381 ttcaattaat aaagtaagta aagataagaa agaaataaga ttatcagaag taaagactcc
    10441 tgaatgggat aaaatatatg tttttaaatc tggaacatct gcagatgata tatataaaac
    10501 aataggatat aaatggagaa atatatcatc tccaaagggt gatgacatgg tgcagattgt
    10561 tgtaatgaat aataataagc ctgttagtta tatatatggt gaagctgaag ttatgccaat
    10621 taatataaaa tgtgatgaat cagagtataa agataatgtt gtaactattt atcctaataa
    10681 aaatgataaa tttaaaattg aaaaaggtaa acaagaatat agtattaatt taaaacatat
    10741 acaatagaaa cttttatctt atttaagttt agattgtact taaaataaag tgtgtattat
    10801 tttaaaaagt gatgataatt aaaatcacaa tattaaggaa tagtacacac tttttatgtt
    10861 aaaaaataga atgttaaaaa attaaacttt taaattaaag tacattgctt atattatcca
    10921 atattatttc caaaggttgt gtaatatttt tatccttatg taatattgct aacattttat
    10981 tttccatatc tatcccatct aattcaatca atcgcaattt tttatgtaag agtgaatcta
    11041 ataataaatt ttctggtaaa atggttatac ctaatcctgc ttctgcagct ctaataagtg
    11101 caaaagaatt aacactttcc caaacaggat aagctttttg gtttgaaaga gataatacac
    11161 tgtccaaagt atcgcgaata gcacttcctt gctcacgtaa caataacgga aacatgcata
    11221 actgtcgtag agataaggtt tgttcagata aaggaaaagt tggagcacat gcaacacata
    11281 acttatagga acctaataaa attgtacgga atgccccctg tggttctata ccttcccaaa
    11341 aacatatatc agcttctcca ctttttaaaa tatccatagc tgaattagca ctttcgacac
    11401 gtacattcac tttaagagat gggaatgaat tttttagtcg acttaaaatt ggtggaagta
    11461 aaaaagaggc aattgtaatg ctagaaacaa tgtttatagg tgtacattcc tctaaacgac
    11521 tgattctttt atctaaattg cgacaagcag ttaagatact acgtgcttct tctaaaagag
    11581 aaatgccaca gcgagtaaga catacccctc gtggtagtct gtcaaataat gctgtttcag
    11641 cttgttgttc aagttcagct atagcatgag atacagcaga ttgtgtgata tgtaattttt
    11701 ttgcagcacc tgtaaaagtg cctgtttgtt caattgcttc taaaatctct aaatatcgta
    11761 tgtttatcat aataaatatc ctcactagac atgaataaaa ttgattaatt ccattaaatt
    11821 ctatcatttt acagattgca aagcaacagg tatactgaat aatatgaagt gaaaagaagg
    11881 aggaatattt tgaatcaaag cagaaaaaca tatttttggc ttttaattat taacttgttt
    11941 attagcacat tcacttttgg tggagggtat gtagttgtac ctatgattag acgttatttt
    12001 gtaaatcaaa agcaattctt tacagaagat gatttgatga gcatggcagc agttgcacaa
    12061 tcaactcctg gagctattgc aattaattta tctgctttgg caggttatcg agtagctggt
    12121 actttagggg ttgtcattag ttgtatagca gctataatac ctccactcgt tattcttgga
    12181 ttagtatcag cattttacac cacttttata tctaatgcca ttgtaactgc agtattaaaa
    12241 ggaatgcaag ctggtgttgc tgcactaatt gtggatttga ttattgactt gtgctctatg
    12301 atattaaaac aacgttctct atttttatca gttatgatac ctattgcttt ctttgcaaac
    12361 tttgtgatga gtataaatgt aatttttata ttatctgttt gctgttgtgt atgtgtaatg
    12421 caagttttct acaagaagag ggaaaataaa tgaatacaat ttggaactta ttttttatat
    12481 ttttaaaaat aggattgttt agtattggtg gtggttatgc aatcatacct ttaatccaag
    12541 agaaagtagt aaatcaaact ggatggataa gtgagaaaat gtttacagat attataacta
    12601 tttcacaaat gacaccaggg ccactggctg ttaatacttc tacttttgtt gggcttcaaa
    12661 ttggaggttt ttggggagca gttgcagcca ctttaggttg tgtattatgt ggtgtagtaa
    12721 tatctctatt tttatataat ttttttcaaa agcatcagaa ctctacttat atatttgaaa
    12781 tattaaatgg tctaaaatca gcttcattag gtttaattat ctcagctgcg gctacaatta
    12841 taatccttgc attatttggg acgaataaag tgtacttcaa ttctatattt gagatgctaa
    12901 actggactgc actaataatc ttttttgtta cactactgat agtacgtaag tggaaaatta
    12961 atcctattgt gattataata ataagtggta tactgggaat atttttttat ttataagttt
    13021 ttgctaagta aaggatatct atttttttat atcttaaatc caaatagcca ataaagtaag
    13081 gctatttgga tttttaataa attctgaaaa gataatcaga aacccatata acatttaaat
    13141 tatatcactg gttttctaca aaccaatcat aagcttcttg gcaaaatttc tcactatcta
    13201 tatagtaact attatgaaca ttattaattt caacaagttt ctttttacat tttagtgaat
    13261 tataatagct atcaaaatat tgggttttaa aatatctatc atgaagtgtt ttaaagacaa
    13321 gagtaggtat gttgactcca tcttttatct tactatcttc tgcaataaag cattctaaca
    13381 ttgtattaat tatctttgta ctatttttcc aaatatgagg atttttcttt tcaaatattt
    13441 tataaaattc attatcatag acttcattat aacctttata tttaggtggt tttactgtta
    13501 tgtgaaaatt aggaagaaac ttaaagaaaa aatataataa tttttatatt ctattttata
    13561 tattattcat attttatcac agataatata ctaaagttaa tttttttatt atatagctat
    13621 tataataaat tttctataat ataagatatt ctataatttt atatctaaat tcatgtgtct
    13681 ttaattgttt ttatataaaa tgtccagtaa tgctatttgg atttattttt atttcatcag
    13741 gagttccaga agcaacaatt ttccctccat ttattccact gtctggacct atatcaataa
    13801 tccaatcaga aacacgtata atctctgtat tgtgttcaat aattaaaagt gaatgttgtt
    13861 catttacaag ttttctaaaa gtaattaaca aattttctat atctttaaaa tgaagtccag
    13921 tagtaggttc atccaatatg tatagcattc gttttttagt ttttgtccct aattcttttg
    13981 ctaattttat cctttgagac tctcctccgc ttaatcctaa tgtattttgt ccaagcttta
    14041 gatatccaag tcccacttca tctaataagg agagttttct atatatatca gagcaatcac
    14101 taaaaaatgt ttttgcttct gctacttcca tgtcaagtat atctgtaatt gtttttccct
    14161 tgtattgtat agacagtatt tctttttgat atctcttacc ttgacattca ttacaagtta
    14221 cccattggtc tgccatatat tgtagaataa tttttgtttt acctaatcct tgacagtttg
    14281 ggcattgccc ctttttgcta ttaaagctaa aatagctttc atccaataat ttttgttttg
    14341 cttgtggagt ttcagcaaat agagaacgaa tttcatcaaa aatattgata tatgtagcag
    14401 gtgtagaacg gctagatttt ccaataggtg tttggtccat taagataaaa tcgtcaatag
    14461 catcaaatcc agtaagttta gtataatttt tatttgggat agatttttgt ttcaattttt
    14521 cttctagagc aggaagtaaa ctgtgaaata caagagaact cttaccagaa ccactaacac
    14581 ctgtaataga gcacatacag tttaagggaa tttctacatc aatatttttt aagttatttg
    14641 catggcatcc ctttaagctt aaccatttac ttggtataat tttattttgt gagattatct
    14701 tattgtatgt actcaaatat ttgcctgtta tagaatttgg attttttata atttcttggg
    14761 ttgtaccttc tgcaatgatg aagcctcctt ttgtaccagc atttgggcct acatcaataa
    14821 tataatcagc agacagtatt gtatccttat catgttccac tactataaca gtattaccct
    14881 tatctcttaa ttcttctatc attttgatga ttagattatg gtctcttgga tgtaaaccaa
    14941 tagatggttc atctaaaata taggttaatc ctactaattc acttcctagc tgacttgata
    15001 atcgaactct ttgtagttct cctccagata aggttggagc tgttctatct gttgataaat
    15061 aactaagacc tacctttaat agataactaa ttcgaatttt taattctgtc aatatatcat
    15121 ttactaaaga tagctcattt tgttgaagtt gatttggcaa ttcacacaac caattccata
    15181 actgttctat cgtcattttt gttagttcag ggaagcgata acctttgatt gtagtgaacc
    15241 tagcttcaat gcacagcaat tcgccaccac atgtattgca aggaattttg ttcatatatt
    15301 gatgcagggt attattttca gaactagatt ctctaaaaag tctttgaata tggttgattg
    15361 ctcctgaagc aggtcttcta atctttgatt ctcgtcccct tgattcaaaa ctaaattcat
    15421 atatcttatc gtctgtacca tataagattg catctataaa atctcttggc aattcattcc
    15481 aaggtatatc taggtctata tttttatctt ttgctattgc atataattcg cctaccatcc
    15541 aattaccatt tggctttttc cctctcaatt ttccaaaata gtaagtagct ccatctaata
    15601 ttgaaatagt tgggtctttt acaattaatt gaggattgac ttgataggta aaacccaatc
    15661 cattacaatc gtgacaagct ccagtatggg agttagcatt gaaggttgaa gtatttatct
    15721 tagaaaaaat agtttggcaa tgtggacaat aattttttgt attattcttt ttttctaatg
    15781 gagttttgca atatggacag taactttttc cgattgatac aaataacatt cttaagtaat
    15841 cccaaatgct tgtaagagta ccaacagtag aacgaggatt ttggcttcct tttttctgtt
    15901 cgatagctat agtaggggtt aatccctgaa gtaaatcaaa ttccggtttt tgaagctgtt
    15961 ctgaaagttt tgctccatta cctagatttt caaaataaca tctttttcct tcttcgtaaa
    16021 tcgtgtcgaa agctaaagaa gattttccac taccacttac acctgtaatc acagttattt
    16081 tattctttgg aattgtaacc tcaatatctt ttaaattgtt ttgtcttcct ccctttaaat
    16141 aaatagaatt tttattatga caaaatgaat ttttaattga tttatttatt gcttttgtaa
    16201 atttgaattt cttttctagt tcaagaggat tgcctaattc ttttatttcc gaaataccta
    16261 atatttttaa ctgagaaaag attgatttca aataatcttc aggcaatttt tctattaaca
    16321 aattaaggtc ttctattttt atatttccgc ttgataatat ctctgccatt tcatctaata
    16381 aatttttttc tgctcttgtt aattgattgt attttttgga acttaattcc gtccataaat
    16441 cccatggaag caattccaaa ccaatcatat tattaaagtc atgtagaaga ttacccttta
    16501 cagaaggcca acctttccaa tttttattat atccaaagtc atctggatgt gcatgtcctt
    16561 gtcgacatct tatccaggct tctgaaccag ttaaaaaacc atctgttttt ccgacatcat
    16621 gagtatcaaa attaatttga aaataatttt tttgtatgtc atctatttgt gcatctattt
    16681 ttatccatct ctttgttaga gtatcatagt attctacaag ccagtggtct tcataagtaa
    16741 gctcactttc aaaataatta gcaaaacctg ctctcattct agcctcaaaa cctcgatatc
    16801 ttaagagtga tacaagaagt aaagaataat ctctgcacat tccaacaagc ctttgttttg
    16861 ggtttcttgg aatagttagt gggttagaat ttatttcaaa tattcttttt aacatttgag
    16921 gaactgtacg aagcaattcc tcatcagatt gttgcttatt aaaggatata ccatataatt
    16981 ttccttggtc tccatgaatt actaggcttt gaaccacttt taataactca actggagtct
    17041 ttggtaaatc ttgatattta ttttcaaatt ttttaggatt tgtgatgatg ctttgatttt
    17101 tatagttatt tattttcatt tatatttcac ctctctaaat ggatgatata ccctgacatg
    17161 gtgtcatata caagggcaat ttagattttt tgaccaaaat ttaaaaataa ataaaaaatg
    17221 atacaatgat aaattgtaat acaaatatag tttcaaattg catataatat aattgtttaa
    17281 taaaaccatt atctatggaa tgttaatgaa atgttaagat ggcattcttg aatattaaga
    17341 aatagaatac tataatatag tgtgaaggat tcttagtaaa aaaatttgta agtgttaata
    17401 tacattaaaa aataggttta taatttggag gaaaaaaatg aaattatcaa ggtataagaa
    17461 aaatagttta gttctgtcta taattttatg ttcacttatt atttcaatcc ttgtaattac
    17521 tgtttttaat accgtaaaac tacacaatat atctaataaa caatctaaaa actatttaaa
    17581 tgatgtatct acacagattg taatgaatgt tgattctaaa atcaaattta ttttatccga
    17641 cttaagaata atggcagatt ttattaagca atatgaaggt gatagtcgat atgagtattt
    17701 atctaaaaga aagactagtt ataagtatta tgatattgga ataatagatt tagaaggtaa
    17761 agctgagttt ttaagtggaa aaaagtttga cttaaagaat actgattcct ataaaaaagg
    17821 aatcaaaggt aaagaatact cagaaataat agagtcaatg ggttttgttc tttatagtgt
    17881 acctatattt aatgataatc atgaagtatc tagtatttta ataggtgtat ctgataaaga
    17941 aagtatgaat gagattctta atatagataa ctttaatgga gaaggaacta ttgaaattat
    18001 taatagtaag ggaaaacctc ttttcatagg aaaaaatact gaattaataa aagaccttag
    18061 ccataaatat aagggttctg gagatgaacc ttgggcacaa aagatgctta atgattttaa
    18121 aaatgataaa tcagggaata ttacaataac ctcatcaaaa ggtgtacaat gcttattaac
    18181 ttatcattca attacaagag gtttaaatga ttggcatttt cttctcatag tacctgaaga
    18241 tgctgtactt ggtgaactta ataagttaaa ttcttttaca ataataatga catttataat
    18301 tacatgtatt attggaatta taacattaat tttatatatt ataagaagaa aatatgtaaa
    18361 tcaaatagaa aatattgcat atttagatag tataacaaat ggtataaatt caactaagtt
    18421 tagtatgcta gtcaaacctt taatttcaga atcacctgat agtacttata tgatggtagc
    18481 aataaatata aaagatttta agctaataaa tgattgtttt ggtagtgaaa agggaaatat
    18541 gacactaaaa catctttaca atgttttgta taaaaatatt gatgaaaatg aagaatttgt
    18601 gtgccgtcat gatgcagacc ttttttattt gcttattaag aatagaccag tgttagaagc
    18661 cctggagtta ttatataaaa tagaagctga tacaaattat tttaataaaa atagagaaaa
    18721 tccatacttt ttaagaatat cagtaggtat ttacacaatt gaaaatcata atgaagattt
    18781 aattacaata caagaccatg caaatacagc aagaaaaagc tttaataaaa ctcatcaaag
    18841 tgatttttct ttttatagtg atatagaaag aaaacgacta atatctgaaa aggaaatttc
    18901 aaatttaatg gaaaaagctc ttgaaaataa agaatttttt atgtgcttac aacctaaaat
    18961 tgatataaaa agtgggaaaa ttagtggagc agagtctctt gtgcgttgga aaaattcaga
    19021 aaagggcata atttatccaa gtgattttat tcctcttttt gaaaaaagtg gatttatatg
    19081 taagttagat ttatatatac tagaagaaac atgcaaattg atttctaagt ggataaaaga
    19141 aggtaaagaa gtgttgagaa tttctgttaa tgtatcacgc caacatttga atgataaatt
    19201 gtttttagaa aaatataagc ttatatgcaa taaatacaat gtaccaacat atatgataga
    19261 tttagaactt accgaatcta ttttcttaga aaatccagaa gctgtagatg taatagatga
    19321 tattcattct aatggattta aatgttctat agacgacttt ggatttggat actcatcatt
    19381 aggaatatta aaggatttta aagttgatat aattaagtta gaccgttcat tctttgtaag
    19441 caaaaataat atagaccgtg gtaaggtagt tattaagtct attattgagc ttagcaaaag
    19501 gctaggtatg aaagtaactg ctgaaggcat tgaagaatta gaacaagttg agttcctaaa
    19561 aaatgtggga tgcgattata ttcaaggtta tgttttttct aaaccattat taatttctga
    19621 ctttgaaaat tttgcatatg aaaataataa aattaaaaat atctattatt aaatgtataa
    19681 gataaaaaat ttagacaagt tatataatta aaaatataac ttgtcttttt tatttatata
    19741 tttaataaaa atgtatttgc agattttgta aaaataattt tcaaatgttg taagttgtgg
    19801 ctaaagcttt attgtattat tctgaatata gaaagatagt tactgtctta gataaaaaaa
    19861 ctaaaacata cataataata taaaatttgc tatattaata actctaatta ttttggaagg
    19921 agggaaaata tgataagtgg gagaatgtta ctcatcatga attttctaaa aggaaaaaat
    19981 aagacaagtt acaaggatat tagcaaacaa cttagtatag aagaaagaaa agttagatat
    20041 gatataaata atttaaatat tgttttgcgt tcattaaaca agtcagtaat acaaaaaatg
    20101 aacaaaggtg tgttaattat tcctgatgac ttccaagtag tatgtataaa taataacgaa
    20161 tatgtatttt ccttaactga gcgagtatct attatgaaaa ttatatcaat gtttaaaata
    20221 gataatctaa acttagaaaa gttgagtaaa gaatttcagg tttctagaag ttcaattaaa
    20281 aatgacctta atgttttgtc aagtgaatta gaaagaaacc aaataactat tacttatgat
    20341 aaagctttta aaataagtgg agaagaagac attatatata agcaaagatt gaacatatta
    20401 aaatcatatt cttatctttt tggtaaggaa aaatctgatt taagtgtatt tgagaagtat
    20461 ttagtgaacg tgattgaaaa aatatttaaa gggatttatt tgattgacat atataattgg
    20521 tctatgaatt tattattgca aatgggatgg actttaaatg atgaatcttt tacatggtat
    20581 gtgtcaaaca ttttcctttt tacatggtat atacattcag atgaaaaaca tcctttagaa
    20641 tctgcttcat taagtgagcc gtgttttgaa aataagtttg accaagaatt tgaaacaatt
    20701 attcaaaaaa agttgactaa ggaacaaatt tttctgttaa agagttttgt attttttaca
    20761 aataaatatg caagtttaaa cgaagatatg gatttgatta gtacagaaat tatagttcaa
    20821 gatttaattt acaatatgtc agaaatatta gagattagct ttaaagaaga tatgattctt
    20881 tataaaggat tactcaatca tattgcccca ttgatagaga gaatcaaaca gaatatacag
    20941 atttatgaag aattactcca tgtaatacca gataaatatc aatatgtact tgaagctact
    21001 aaggtttcta ttcaaagaaa cagagtttta agtcaaataa agaatgaaaa tgaaacaata
    21061 cttttggcaa tacatttctt aggaagtata catagaaatg agaggaataa ctacaaaaat
    21121 atattgttag tttgtggatt tggatatggt gtaattgcta tgttaaagga tagtttggta
    21181 aatgattacc agataaatat agtagagagt attccatcat ataagttaca agattataaa
    21241 gactgggaaa gtattgactt cattataaca acatcaaaga tagtaataga cttgcctaaa
    21301 acaattattg agataaatcc atttttacaa gaagaggatt atttgaagtt tgaagaaaaa
    21361 gggataaaaa gaaagaacgt tttaactaat tatatgtcaa taaaaaaaag gttggatttt
    21421 ttagaagaag atgcccaaaa aagagtgttg agtattatat gtcaagaact tggatacagt
    21481 gatgagagaa tattatttag acaacttaat ttaagtgatt taatagggat tgataccata
    21541 aggataattg atgaagagat gaaatgggaa gatgccgtaa aaattagttc aaatatactt
    21601 gctaattgtg attttgtatc agcacaatat gctgaaaata ttatagatat tttaaaaaat
    21661 gtgggttttt atgctgtaaa agataacgaa tttgctttac tacatggcaa tgatagttca
    21721 ttagtcaaag taagttcgat aagtttatta atatgtaaag aagcagttag ttttagagat
    21781 aaaaaggtta aaatcatatt ttgtctagca agcagagata aaaaagagca aattcctgca
    21841 ataatcaatc taactaaaat ggtttataag actaatttta ttgctatgtt agaaagtgca
    21901 aagacaaaag aagaagcaga agttttaatt catagatatg agaaggaggt cactgtatga
    21961 gtaaattagt taatgtaaaa tgttcattta aaatacatgt taattcaaaa gaagaagcca
    22021 ttatatcatt agttgatgta gtgtcaaaag aaggatattt aatagataaa aatcaatttt
    22081 tagaagatgt tttaaagaga gaagaaacat tgtcgactta tattggacat ggtataggtc
    22141 ttcctcatag tcaaagtgta ggtgttaaaa actcttgtat tacaatagga aagcttgata
    22201 ctcctattga atggacagaa gaaggagaaa aagttgattt aatattttta atatcagtaa
    22261 caaaagacaa tgaaaataat cttcatttaa agattttatc aaaattagca cgtttattga
    22321 tgcatgaaag ttttagaaat caaattagat gttcagatga acaaactgtt tataatctta
    22381 taaaagaaaa aatagaggag gaagattaaa atgagaattt taaagttttt gcaaaaacat
    22441 cttatgacag caacatctta tatgattcca tttgtggttg caggaggaat attatttgct
    22501 ttatcagtaa ctctaagtgg acaagctgct gttcctgaaa caggatggtt ggctaaactg
    22561 aatcaaattg gagctgctgg attggcttta ttcattccta tattaggtgg atatatagca
    22621 tttagtatgg cagataaacc aggcttagct ccaggtatga taggtgcata tttagcaaaa
    22681 gaagtaaatg caggttttat aggtggtatc atagcaggat ttatagcagg atttgttgtt
    22741 ttacaattaa agaaaattaa attagctcct acaatgagaa ctttaggttc tatatttatt
    22801 tatcctttga ttggtacatt gattacaggt ggtataattg tatttttaat tggagaacct
    22861 atagcttctt ttatgacatg gatgacaaac tggttaaatg gaatgtctgg tgtttctaaa
    22921 attcctttag gtggaatttt aggaggaatg atagcctcag atatgggtgg tcctataaat
    22981 aaggttgctg ctacttttgc ccaaactcaa gttgacacac ttccatattt aatgggtgga
    23041 gttggagttg caatttgtat acctccaatt ggtttaggat tagccacatt attgtttcca
    23101 aaaaaattta gcaaggaaga aagagattca ggtaaagctt cgctattaat gggatgtgtt
    23161 ggaattacag aaggagctat tccatttgca acatcagacc cagttagagt aataccttgt
    23221 attatggctg ggtcgattat aggaaacatt atggcttttt tattaggatg tttaaatcat
    23281 gcaccctggg gaggtctgat agtattacct gtagttgaca ataggttggg gtatattgca
    23341 tcagtattaa ctggagcagt agttgttgca gtattaatga aattagtaaa aaaagacgtg
    23401 aaagaagatg aagaaattga agaggattta gatgactcaa ttgaattaat atttgaagaa
    23461 ttataaatat aaatggaagg aatttgtata aaaaagtaaa tataaaacta taaatttaaa
    23521 ggagataagt atatgaaaat tgtagcagta acatcttgtc ctagtggagt agcacatact
    23581 tacatgtcag ctgaatcact tgaactatca gccaaaaaat ttggtataga aataaaggtt
    23641 gagactcaag gaagctctgg aatagataat gaattgtcac ttaaagatat tgatgaagca
    23701 acttgtgtaa tactaactaa tgatgttgag attagaaata tggaacgttt taagggaaag
    23761 aaggtagtaa gaatgagtgt atctgacata attaagaaat ctgatgcatt aattaagaaa
    23821 ataaaagata cttttcaata aaaattgatt tattttacat ataactcaaa gatgttttta
    23881 aagaaatttt tattaaaagc atctttgaaa atatcttaaa aatagaataa aaggagatat
    23941 agtcatgaaa aagttaccaa taaagactgt cgtacaaaga ttactagact tacaagaaga
    24001 ggggaaaagt gcaactcttt taggaattgg tcctatgtca ccaaacttgt tacaagcaag
    24061 ttttgaacta gcaaaagatg atgattttcc tttaatgttt atcgcaagta gaaatcaagt
    24121 agatgcagat gaactaggtg gcggttatgt aaatggttgg aatcaggaaa cttttacaaa
    24181 agatattaag aagattgctg acaaagttgg atttgatggt ttatattatt tatgtagaga
    24241 ccatggtgga ccatggcaaa gagataaaga gagaaatgac catttgcctg ttgatgaagc
    24301 aatggagtta ggaaaaaaat cttatttagc ggacattgaa gcaggttttg acctattgat
    24361 gattgaccct acaaaagacc catttgaaat tggaaaaata attccactag atgtagtact
    24421 agaaagaact gtagaattaa ttgagtattg tgaaaacgag agaaaaagac ttaatctacc
    24481 agacataggt tatgaggttg gtacagagga aaccaatgga ggtctgactt caattgagac
    24541 atatgaaaca tttataacta gactaaaggt tgaacttgat aagagaggtt tgccaatgcc
    24601 aacatttata gttggtcaaa ctggtacttt aactagaaag acagaacaag taggaacttt
    24661 taattttaga aatgcttatg atttagctca aatggctaag aaatatggag ttggtttaaa
    24721 agaacataat ggtgattact tagatgatgt aactttatta gaacatatac catctcaaat
    24781 tattgctaca aatgtagctc ctcaatatgg tactgaagaa actagggcat atttaaaatt
    24841 agcagaagtt gagtgtaaat tagaaaaaga gggtctagtt gataaaactt ctaatatcag
    24901 acatgttctt cttgttaatg caattgaatg tggacgttgg agaaaatggg taataggaga
    24961 acaaaaaaat cttactactg aagagatatt taagaatgaa gttttatcta atgaaatatt
    25021 agatattgca ggacattata catttaataa tgatgatgtt aaaaaagaaa tagaagtact
    25081 ttatgctaat ttaagtaaga ataatattga tggtcaaaga tttgttgtag accatattaa
    25141 acgtcctttg cgtgattatg cagagtgcta taacttaaaa ggtgttactg cgaggatttt
    25201 aaataaataa taggacttat agtggtgagt attgttatac taggtattta tagttataat
    25261 tatacttacc actattaaat ttaggaggac taatattttg attaatttaa tttttgatgt
    25321 agatgatact ttatataatc aattaacgcc tttttacact gcatataata aagtgttttc
    25381 ttcaataaag gatatttcta ttgaagattt atatatgagt agtagaaagt atagtgatga
    25441 ggtatttcat atgactgaga atggagaaat gccaattaaa gagatgcata tctatagaat
    25501 aatgaaagct tttgaggagt tgggaaattc tattacagaa aaagatgcac aaagttttca
    25561 agatgagtat atatatcaac aatcacaaat tacactaata ccagaagttg agtgggtttt
    25621 aaatttttct aaagaaagaa atattaatct aggaattata actaatggtc catcaaccca
    25681 tcaaagaatg aagttaaagc agttaaatat tgaaaactgg gttgataaaa gtaatatttt
    25741 tatctctagt gaggttggat tttctaagcc agatgctaac atttttagag tagctgagaa
    25801 tgttatgaat ttagatagag aaaatacata ttatgtgggt gattcttata gaaatgatgt
    25861 attaggtgct aaaaaagctg gttggaagag tatatggttg aaccataggg gacatgaggt
    25921 agaagatttg ttttataagc cggattttgt aattttggaa tataaggatt tgatgtcttt
    25981 atttataaaa atatgtagtt ataaaaatat gtagtaaaaa aatgttaata atagaattgt
    26041 ataaaatagt taataataac tatataaaat tatatattta gattgtgagc aatctatttt
    26101 atgttaaaaa tagattattt agaagaaggg gaaatcttaa aaaaatttga atatcccctt
    26161 tttattattt aattaaaatg aaatagttag aaatatattt tatttagatt aatcaataac
    26221 attttgagat tctttataac tacatgttaa ctgttgaata ggtttatcat atggtttaac
    26281 ccatccttta ttaatactta attctgtaag tgctgtgtga ccttctacca tttgagttag
    26341 tgaagctgaa tatatagctc ttagttcagg tgtactactt gttaatgcag aattaagata
    26401 catattggca gatgctttag cagatgaaag catacttaac attattactt tatcgtctat
    26461 ataagtattg ttcttaatta aattttctat catatttctc atgttatcta tacctcctca
    26521 gaaattagta tttcattttc aactataaat tgttgtatag ctttaattct tccttctgtt
    26581 gctaatatac tagattctga ttgtcttttt aggtcttcat ctgatattaa catatttatt
    26641 gctctttgca taagtaatcc atcagattcc atttttataa ctgctgctaa agatattttt
    26701 tcagtttctg aaagtattct catttaatca tctcctatat tgtaatttat gctttaatag
    26761 tatgtctttc ttttcaacga aataaccata acactttttt acagtatttt atattctctt
    26821 tatatcaaag cttagttagc ttatatacta ataaaatttt gtaggagagt tatcattaaa
    26881 tacttataag ataaattatt taaatatcca ttaaataaaa tttatttgta tgtacttctt
    26941 caagagtcaa tatgagtttt ttcaaaaaat tacttttgac aataatcatt aagaggtata
    27001 ttttcttgca gttgtgatta ttattattaa tttactaaaa tactttaatg aaaaaatgga
    27061 taactttaat tgtaggatat tattaatata tttgaaataa ataaaaatat tgaaggatat
    27121 aatattcaat tggtatataa ataataactg atagttaaat tttactttag agtctaatta
    27181 ggtgcttagt caaagttaat gcctttagaa tacaaatgga ttctaaaggc atttttacta
    27241 tgtatttttg tttatattac taaatattat ctatttttat atacatttct atgtatttta
    27301 tttttatcaa atcggtctat aaattcattt ttttcttcac tatatccaat agatataagt
    27361 gcataaggaa ctaattttac tggtaagtct aaagtttgtc taactttatt tactacttta
    27421 tttttaggaa atactcctgc ccaacaagca gctagacctt gattagtaac ttctaataat
    27481 aaattttgtg tgcaagcacc taaatcttgt atccacttac caggttttaa gaatttatct
    27541 ctatttccaa gcactactat acataatggt gcaaactcta catgctttgc tctatgctgt
    27601 gactttgaga gctctttcag tagttcctta tcatctacta taataaattc ccaaggctga
    27661 gcgttttttg atgaaggtgc ttgcatagct gactttagta tttcagttat tttctcttct
    27721 tctatatttt tatctttata tgttcttatt gaacgtctat tttttataac ttctaacata
    27781 cagattctcc tttaaataaa a
//
LOCUS       DAEMAE010000026        27639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEMAE010000026 DAEMAE010000000
VERSION     DAEMAE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.026"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..487)
                     /locus_tag="KPH93_000928"
     CDS             complement(<1..487)
                     /locus_tag="KPH93_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687762.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RI"
     gene            complement(819..1601)
                     /locus_tag="KPH93_000929"
     CDS             complement(819..1601)
                     /locus_tag="KPH93_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG1687763.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKSNDNYIME"
     gene            complement(1604..3823)
                     /locus_tag="KPH93_000930"
     CDS             complement(1604..3823)
                     /locus_tag="KPH93_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG1687764.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(4087..5184)
                     /locus_tag="KPH93_000931"
     CDS             complement(4087..5184)
                     /locus_tag="KPH93_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687765.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(5500..6693)
                     /locus_tag="KPH93_000932"
     CDS             complement(5500..6693)
                     /locus_tag="KPH93_000932"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG1687766.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(6829..6932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7121..7630
                     /gene="yyaC"
                     /locus_tag="KPH93_000933"
     CDS             7121..7630
                     /gene="yyaC"
                     /locus_tag="KPH93_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG1687767.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(7741..8169)
                     /gene="fabZ"
                     /locus_tag="KPH93_000934"
     CDS             complement(7741..8169)
                     /gene="fabZ"
                     /locus_tag="KPH93_000934"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG1687768.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(8185..9204)
                     /locus_tag="KPH93_000935"
     CDS             complement(8185..9204)
                     /locus_tag="KPH93_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG1687769.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(9401..9661)
                     /gene="spoIIID"
                     /locus_tag="KPH93_000936"
     CDS             complement(9401..9661)
                     /gene="spoIIID"
                     /locus_tag="KPH93_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG1687770.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEREHKKM"
     gene            complement(9759..10427)
                     /locus_tag="KPH93_000937"
     CDS             complement(9759..10427)
                     /locus_tag="KPH93_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG1687771.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(10493..11557)
                     /gene="spoIID"
                     /locus_tag="KPH93_000938"
     CDS             complement(10493..11557)
                     /gene="spoIID"
                     /locus_tag="KPH93_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG1687772.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKVPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIMTYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(11644..12897)
                     /gene="murA"
                     /locus_tag="KPH93_000939"
     CDS             complement(11644..12897)
                     /gene="murA"
                     /locus_tag="KPH93_000939"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG1687773.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(12918..13655)
                     /locus_tag="KPH93_000940"
     CDS             complement(12918..13655)
                     /locus_tag="KPH93_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG1687774.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            complement(14119..15414)
                     /locus_tag="KPH93_000941"
     CDS             complement(14119..15414)
                     /locus_tag="KPH93_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG1687775.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(15908..17740)
                     /gene="glmS"
                     /locus_tag="KPH93_000942"
     CDS             complement(15908..17740)
                     /gene="glmS"
                     /locus_tag="KPH93_000942"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG1687776.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIVLDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(18007..19353)
                     /locus_tag="KPH93_000943"
     CDS             complement(18007..19353)
                     /locus_tag="KPH93_000943"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG1687777.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDRNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(19523..20080)
                     /locus_tag="KPH93_000944"
     CDS             complement(19523..20080)
                     /locus_tag="KPH93_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG1687778.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(20081..20833)
                     /locus_tag="KPH93_000945"
     CDS             complement(20081..20833)
                     /locus_tag="KPH93_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG1687779.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(20833..21912)
                     /locus_tag="KPH93_000946"
     CDS             complement(20833..21912)
                     /locus_tag="KPH93_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG1687780.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKREPNVINSLYLEPEV
                     LEDHCWHLQEKFNAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(21941..22156)
                     /locus_tag="KPH93_000947"
     CDS             complement(21941..22156)
                     /locus_tag="KPH93_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1687781.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(22199..22876)
                     /locus_tag="KPH93_000948"
     CDS             complement(22199..22876)
                     /locus_tag="KPH93_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687782.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAISAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(22866..23945)
                     /gene="buk"
                     /locus_tag="KPH93_000949"
     CDS             complement(22866..23945)
                     /gene="buk"
                     /locus_tag="KPH93_000949"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG1687783.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(23967..24869)
                     /gene="ptb"
                     /locus_tag="KPH93_000950"
     CDS             complement(23967..24869)
                     /gene="ptb"
                     /locus_tag="KPH93_000950"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG1687784.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDIREPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(25173..26354)
                     /locus_tag="KPH93_000951"
     CDS             complement(25173..26354)
                     /locus_tag="KPH93_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687785.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKINYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(26351..27190)
                     /locus_tag="KPH93_000952"
     CDS             complement(26351..27190)
                     /locus_tag="KPH93_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG1687786.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT         8289 a         5293 c         2995 g        11062 t
ORIGIN      
        1 ttattcttat gtctttctca tccccttcaa tatatatacc actgcctttt ttagtcgaga
       61 catttaaatt ataattattt aatgccactg ataatttact tatttcttct ctgacaatat
      121 ttttatttat gtacaactta ttagataaat catttaaagt aactttttta ttattacaaa
      181 gtagtaaatt taatatctca acttgcttat gaagctcact atttactctt ttaacttcac
      241 ctcgcaactt ctgatttacc agtattttgt ctgttccatc tccactaaat ttaattccaa
      301 tattaggttt tcttttgata taagcattac aaaaagtaga taaccattca tcaatatatt
      361 tgcaatcatt tcttattgtc ctttctgaac actcaaaatc attggcaatc ttattaacat
      421 ttataaactc ttcttttaat agcaacgttg ttaataaatt ttcctgtctt ttattcaaac
      481 atatcacctc tttatgactc agcctaaatt tattatatat aaaaaagagc tatacataaa
      541 ggtatgctag ctccctttta atacgatatt tagctgacct ggtttttgac cccacactcg
      601 cctactggag ggttcgcttg ggttcgccgc actactactc tctctcgatt ttattatcgg
      661 taggtcttac ttctaagccg caccatgatc attaacccaa tttttagtgt taacttacgt
      721 ggatcccttt gcctgcgact ggcttggact ttcaaccaca gacctaaata tcacttattc
      781 attttaatat tttaaattaa aattgtcaat atataagatt attccataat ataattatca
      841 tttgattttg taagtagagt caatacaaaa accttattag cacctgataa ctttaaaagt
      901 ttagaaattt cgtttgttgt tagaccagta gtaaatatat catctattaa aagtatattt
      961 ttattattta ttaatttaac attttcttta acaacaaata cattttttaa ttcttctttt
     1021 cgttctttct tattcaattt atatagcatt cttgtatatt tttttctact tatacaatcc
     1081 aataatggaa tatttactat tttacttaaa ttaaatgcta ttttttgtgc ctgattaaaa
     1141 cctcttttat ttagcctttt tttatgaagt ggaacaaata atatataatc aaattttatg
     1201 ttttctaaat ataatttttc tttcattatt tgagctatat acttagccat aaaggttttt
     1261 tggttatact tcagccctag cactatcttt tttgtcacat cattgtattc aatacaagag
     1321 attgatttat caaaataaaa gtctttatta aaacaataac tacactcttc aataaattct
     1381 ttttcaattg aatgacgtat tattggtttt ccacacttca tgcaaccatc ttgtataaaa
     1441 ttcatttctt taaaacaact tttacatatt gaataagtat ttgttttctt tatggattta
     1501 tcacaaatta tgcaacttat attctcagga tatataaaat ccaaacattt attaatcata
     1561 tttttaaaat tcttaataaa attatcattg catattttca tatttattct atcaacagcc
     1621 cttcttgctt aaatttattt aacttatatc ctaaatttga atacctttga ttaactctat
     1681 tattttttat catatattca agatatttca catctccaac taatacaact aatttttttg
     1741 ctcttgttac tgctgtatat aataaattac ggctaagtaa cataggagga gcccaagcta
     1801 ttggcaatac tacaactgga aattcacttc cctggctttt atgtattgtt gtacaaaaac
     1861 tatggtcaat ttcatctaat tcatcatata ggtatgatac aattttagtc tggtcaaata
     1921 atatataaat agttttctta tctttatcta tgtgataaac atatcctata tctccattat
     1981 agataccttc tccactttct ttttggtctt ctgtttccca tttttttgta taattatttt
     2041 ttacttgcat taccttatcg ccaactctaa ataatctttt agaaaaactt tcttcaactt
     2101 taaatttttc ttttttgtta agatacttct gtaactctat attgagatta gtaactccca
     2161 attcaccttt tctcattgaa gataaaactt gtatatcttt taatttatca actttataaa
     2221 atttaggtaa tctttcatta accaatccta ttatttcatt taaaatttct tcattagttg
     2281 attttcttat aaaaaagaaa tctttacctt ttgtatttag ataaagtggt tctccattgt
     2341 ttatcttatg ggcatttaca attatcatac tttcttgcgc ttgtctaaat atttcattta
     2401 atttaactac atttataata ttagaattaa tcatatcctt aagtacattt ccagcaccta
     2461 cagaaggtaa ttggtcacta tctccaacta aaattactct tgttcctaac tttatagctc
     2521 taagtaaatt gtacattaag ataatgtcta ccattgatac ctcatcaaca attattacat
     2581 ctgaatttat tgggtcttct tcatccttaa aaaaagttaa atcatcatct gttgaaaatc
     2641 ccatttctag caacctatgt atagtttttg cttctttatt agaagtttca ctcattctct
     2701 ttgctgctct tcccgttgga gctgccaaag tcacgctttt accattattt tcaaatatat
     2761 ctatgatagc attaatggta gttgttttac cagttcctgg accaccggtt ataattaata
     2821 ctccactatt aacagattct ttgactgcta aaatttgttt ttctgctaac ttaattttct
     2881 tatcttcttc taaaacatta atctcgttat ctatatctat ttttaagtct tcaaattcat
     2941 actgtgataa ttttactatt tgactacata ctccattttc agctaaatag tatggcataa
     3001 ggtatataag attttcattg tttactttct ctatatgtat tttttgatta taaactagca
     3061 tcattacaca ctcttttatt atttctccat ttagctccaa taatttttcc gaatcttgaa
     3121 ttagaacatg ctctggtaaa tatgtatgcc cattacttaa agatttattt aaagtgtata
     3181 agattccctg acaaattcta tccttagagt ttttgtctat accaatttta tttgctatac
     3241 tatcagctac tttaaatcca attcctctta tatcttcagc aagctggtat gggtttttat
     3301 ttataacttc tattgcactg cttttatatt ttttatatat ttttaggcag taatttggtg
     3361 ttattccaaa aggagatagc tgtattatta tatttctcaa ttctctgtct tcttcataac
     3421 tttttactat ttgttttact tttttactcc ctataccttc tacttcttgt agtttttctg
     3481 gtgaattttg tatcacctct aatgtatcta caccaaactt atcaattatt cttttagcca
     3541 ttttttcacc aataccatgt atcattccag aagataagta aacataaatt ccttctaagg
     3601 atgatggtgt tactggcata aaactattca cttcaaattg agtcccataa atcttatggt
     3661 ttacccactt tccttcaact tctatgcttt ctccaattgc caaagtaggc atacatccta
     3721 caacgacaat ttcatcattt tcatttacca gatgagctat tgtataacca ttatcttcgt
     3781 ttttaaagac tatttcactt atcattcctt ctaatttttc catttttatc tcctaaaaat
     3841 actaataatc gctccattta aatgttagta taattttata tattaaaact atgataatcg
     3901 tagaaaagtt ttatattttt atacttaacg taatttccaa ctattataca ttttatcaga
     3961 aacaaaaaaa atctgcatcc ataaaatatg gtaccatgaa atacagatac catatttaaa
     4021 tagctatttt attttaatta ctccatattt agagtataaa ttaaatttca taagtatatt
     4081 acagtactag cttatttcag atttattctc cttgtacccc tcagttttag taattcttct
     4141 ataaagcata catagtaaaa ttaaaattcc catgtaacct gtatatggat atagtgtacc
     4201 tactaactta tcaaatggca gtagacctcc tatacaagct aatatactta ctactattgt
     4261 tataatttta aatttagggt ggtcatcttc tacaaatcgg ttagttactg accataacaa
     4321 tggtacagct gtagtgtata ttcctagtaa aagtactact gagaataaaa tacctattat
     4381 tggagatatc ttgtcagcta agtaaagtgc tggtatctct tttgtatata tatttcctat
     4441 gtctgaaagt aatgccaaat tcatcattat agctgctgcc ataagtgcta ctccacctac
     4501 tataccaccc cacacagcat ctttcttatt tactgcagaa gctcccattc ctgtcaagaa
     4561 tgttattact attattacat tataacaagt ataaagtact cctgagaaat atccatttga
     4621 tgtagcagtt ttaatattta aactatcaac catatttcca gcatttgata aagcatcaat
     4681 attgctaaat aaactaattg ctcctactaa aagtgtaaat attattatta taggtcctat
     4741 attacctagt attttagaaa gccttgtcaa acctaatgaa acagttatta gggaaacgat
     4801 tgccatacca atcctaccta catatggatt taatccatag tactctgata atgtagctcc
     4861 tgctcctgca atcattatta ctaatgttcc aaataaaaac aatggaccaa accattcaaa
     4921 aaatgttcca agatattttc cacaatatac ttgataaatt tttattggct ccttaagttt
     4981 caattctctt cccttatcta taacttctgc tccaaaccat gaaaatagta ccatagagat
     5041 tattccacct ataataccag catatccata aaacgtaaaa aattgcagta tttcttgacc
     5101 agttgcaaat ccagaaccta ttacagtagc aacataagct ccagcaaaac ttataaccgt
     5161 tttaatattt actttgcctg acatacacta ctccccccca attaaaatta tttttaaagt
     5221 gtaagtttat taagattata ttagactcat tattgtgata tttcaatata tttttaatta
     5281 tttaacatta gttaacacat ttttaaccat cctttaacat atatcccata atatgcataa
     5341 atttagtata gtattatata aaatgtattg accagattaa atactgttta ttcattatat
     5401 gatgataaat taatatttat taaaaatatt taactcataa taaaaaagct gttagaaagt
     5461 aatcataaaa ttacattcta acagcttttt agttaaaaat tattcaccta aagcatcttt
     5521 tcttaactgc tcaaccttgt cagttctttc ccaaggtaaa tctatatcag ttctaccaaa
     5581 gtgaccataa gcagcaacct ttttataaag aggctttctt aaatctaagt ctcttattat
     5641 ggctcctggt cttaaatcaa agtgcttatt tactaattca acaagttttt cttctgaaac
     5701 tttgcctgtc ccaaaagtat ctataaatat agataatggt ctagctatac ctatagcata
     5761 tgcaagttct atctcacatt tgtctgcaag accagccgca acaatatttt ttgcaacata
     5821 tctagctgca taagcagcag atctatcaac ctttgtaggg tcttttcctg agaaagctcc
     5881 tccaccatgt ctagaatatc caccataagt atctattatt atttttcttc ctgtaagacc
     5941 tgtatctcct tgtggtcctc ctataacgaa tcttcctgtt ggatttatat aaattttagt
     6001 atcttcatca agtaattctg ctggtataac ttctttaata acatgattaa ttaaatcttc
     6061 tcttatttta tcatttgata ctgtttcaca atgttgagct gatataagaa ctgtatgtac
     6121 tcttacagct ttacttcctt catattcaac agtaacttga gtttttccat ctggtcttaa
     6181 ataatcaact agaccagtct ttctaacctc tgtaagtctt cttgataatt tgtgtgctaa
     6241 agatattgga agaggcatta attctggagt ctcattacat gcaaatccaa acattatacc
     6301 ttggtctcca gctccaactt tttctatttc ttcttctatc tcttctccag tcttactttc
     6361 taggccttca tcaactccca tagctatatc tccagattgt tcatcaatag aagttataac
     6421 tgcacaagta tcacaatcaa atccatattt tgctcttgta tatccaattt ctcttactgt
     6481 ttctcttact aatttaggaa tgtctacata agcagatgta cttatttctc ctgctactaa
     6541 aactaaacca gtagtaactg tagtttcaca agctactcta gattgtgggt ctttttctaa
     6601 taatgcatct aatattgaat ctgatatttg gtcacatatc ttatcaggat gtccttctgt
     6661 aactgattct gacgtaaata aatgtcttgc catttttatt cccccttatt tattcttagt
     6721 ttttattata ggttttgcct tgtttttaat tttagaaatt tactcaaaat aaaaaacctc
     6781 tcctttataa gaagaggtta ataatcgtaa ttacatacta attaactgac ctcatctttc
     6841 aagacttttg tcttgtagga attagcacct tttcccattt gggtggttgc tgggcttcac
     6901 agggcctatc cctcagcctc tcttgataag gtaatcaaga tttaagtttt gttttacttt
     6961 actagcttac tattttttta aaattttgtc aatattttta gtcatatttt ttctattcct
     7021 ttttttgtaa gacaaataaa attttctatc tatctgaaat ttaaagttag gtctttttaa
     7081 tgctttttat tcatatattt ttttgaaggg ggtgttccta ttgtatttag ctaaagtaaa
     7141 ttacagcgat gatgatgtta tacagatgct aagttcagtt ctaaaaacta taattaatga
     7201 aaatacgatt gttgtttgca taggaacaga cagggctata ggtgatactt taggcccttt
     7261 agttggaaca atacttaaga acagtaactt taaatacccc gtttacggta cactagataa
     7321 ccctattcac gccttaaata tatatgagtc tttagatact ataaaaaata cacatataca
     7381 aggtaacttc ttggctatag atgcttgctt ggggtcgcag agtaacatag gtaatataca
     7441 gattagagaa ggacctatcc tgcccggaaa aggtgttggc aaaaaactac ctcaaatagg
     7501 aaattattct atagtaggaa ttgtagataa aattgatgaa aataataaac tttcatttaa
     7561 taatatacgt cttagcttta tattagactt agctgaaaca atagccttgg ctttattagt
     7621 atccacctaa tctaaatata aactaaatga taaaattctt tatataaaaa ggtagatatt
     7681 ttagagtatc tgattaaata ctccaaatta tctacctttt tacttttata tttcaagttt
     7741 ttatttttct actaaggcaa acattaattc ccctttacag acaagttctt caccaacata
     7801 tgccttagca tctgccttgg ctatatttct tctaagagtt gtcatttcaa cttccatagt
     7861 taatgtatct cctggtctta cttctctctt gattctaact ttatctatac ctgcaaatac
     7921 acctagtttt cctttgtttt cctctacaga catcattgcc actccacaca cttgtgctaa
     7981 agcttctact attaatactc ctggcattaa aggatattct ggaaagtgac cttggaaaaa
     8041 tggttcatta actgttacat tctttatacc aacagctctt ttcccaactt ctagctctgt
     8101 aatcttgtcc actaataaaa aaggatatct atgtggtatt aattttttta tttcatctat
     8161 atttaacatt ctaaactctc ctaattattt tcttttgaaa gacccgccag tttcaagtag
     8221 gtctattgaa cctaaagcta ttcctgtacc ttttgcaaca caagataatg gttcatctgc
     8281 tattgtaaca gttattcctg ttctttgttc tattatttta tctaatccat ataataatga
     8341 acctcctcca gtcattatta taccaacatc tcctatatct gctgctaact caggaggcgt
     8401 tttttccaaa acagcatgag tagcaactac tatttgctca acacattctc taagtgcttc
     8461 taacatttct gttgaattta tagttataga actaggaagt cctgtaatta aatttcttcc
     8521 tgttatcttc atatattttt cttcttctct cttaaatgca gaacctattt caaacttgat
     8581 tttctctgct gttctttctc ctataagaag attgtgttgt tttttcatat atttaactat
     8641 agcatcatca aatctatctc caccaacctt tatagattca cttacaacag ctcctcctag
     8701 agatattaca gctatatcta cagtacctcc accaatatct ataatcatat taccgttagg
     8761 ctgtgatatg tctacaccag ctcctatggc tgctgctatt ggttcttcta ttatatatac
     8821 ttctctagct ccagcttgtc tcgtagcatc ttctactgct cttttttcca cttctgtgac
     8881 acctgttggt acgcatacca taattcttgg cataaaaaat cttccaaatc cctttttatc
     8941 aactatctta tctataaaag attttaacat cttctcagtt acatcataat ctgaaattac
     9001 accatctctt aaaggtctta tagcgactat attacctggt gttctcccta tcatcttttt
     9061 agcttcttct cccacagcta aaacagaatc tgttctattg tctatcgcca caacagatgg
     9121 ttcttctaaa acgattccct taccactaac ataaacaagc acatttgcag tacctaaatc
     9181 tatgcctata tcagctccag ccattttttc aactccttta tttataatat tagattatat
     9241 tataagccta ttttaatact cctataataa agcatataca aaaatctcgc tttaatcaag
     9301 agaaaataaa tcttttgaga gtattattat tttgctattt ataattactt aatatatttc
     9361 tttttataat ttaattaaat aaaaaaagta ggtttttaac ctacattttt ttatgttccc
     9421 tctcatattt taatttcgtt gctaatcccc ctctaatatg tctctctgat ttatttttgt
     9481 ccagcacatt tttaacttct ttggcaaggg atggatttat ttccttcaat ctttctgtaa
     9541 catccttatg aattgtactt ttacttactc caaatgtctt ggcggtttgt cttactgtag
     9601 tgtttttttc aagtatatat tttgctacca ctatggccct ttcctctata tgagatctca
     9661 aagattaccc ccctcacata atagctttaa tgatatatat gtaaaaacac caaaaaatat
     9721 aacaagctta accaaatatg attaaacttg ttatactttt acttaattag actcattggg
     9781 tctatagatt tttctccact atacgcctct aaatgaacat gtataccttc ttcactttct
     9841 atttgagaag tactacctac agtacctaga gattgtcctt ctgttacctt ctgttctttc
     9901 ttaacactaa catttttatc tagatttgaa tatactacta caatgttatt gtcacttttt
     9961 attttgactg attgcccata ttcttcatca ttaaatacat ctactactat tccgtttaat
    10021 agagatttta cttctttgcc tttagtacaa ctaatatcta cacctttatg agtttcccaa
    10081 acatcaagtg ttttagaata actaggttct ttttctgaat atcctcttat tactttatct
    10141 ccaatgtaat ttaattttgt tgtactcgat ttattttcct cttttgactt agctttttct
    10201 aagttttgtt ttgaatccgt tgcagtaggg atagcatctt tcttgtcttt ttcaattaaa
    10261 tgtatttcct cttcactatc tttattagca ttttccataa ttcctttatt agaagccaat
    10321 ttatctacat tattatttgt aaaccaaact ccacctactg ctaataaaca aacacataca
    10381 aacaaagata aataaaaacc atctttttct aacagctttt tcttcatttt gcacctccaa
    10441 atatatttct acatttggat tattgacgtg tttttggcat tttatacaat tattagtata
    10501 tatcttttat ttttgtatct gtatagtaat gtgataatat atcataatat ttatagccct
    10561 cttctgccat tccttctgct ccccactggc tcataccaac accatgacca tatcctttta
    10621 ccacgaaatc tatgtatcct tctccaaatt ttatgtcaaa gtttgcagag ttcaatttaa
    10681 aaacagttct aatgtctttt ccagtcaatt ctttatttcc taattttatt ttttctacag
    10741 ttccggcatc acttctttta gttattgaaa cttgtttact taaattattc acatctatca
    10801 caatagtgct atatgccctt ctaagacttt taacaaaatc tgtgttgctt attttaagtg
    10861 ttgacgcaaa tttaggggaa tacttatcat aaggactttc cacagacttt aaataaggat
    10921 attttgcaga aaatacttct tcactatttt ctgtttttcc tgaagatgta gaaaaataaa
    10981 gaggaagtat cgccttatca ttataagtca ttatttgacc tttagttcct ctaactgctt
    11041 cttggatttt ggaatattta ttcttaatcc attcttcacc atttaatttt ttaagagtat
    11101 cataactctt atattcttga caatgtttat aatcagtaca tactactgca tttttatgtt
    11161 tactagattt accatgttcc tgtttatata ctacatatgt tctagctgct acagattgag
    11221 cttttaaagc ttctatatca aactctgaag acatctcacc agctagtact ccacataaat
    11281 agttctccat gtccatcttt tcggttttcc ctgttatatg attatataca ttgataattg
    11341 gtactttttt atttacagtt tcatagttca ctgtattttt ttcttcttta ttacctttgt
    11401 cttcaatatc acttttttta atagttttgt ttatagatac tgggctctct gctttttcag
    11461 ttaattctac atttttataa gaaactaaag ttataagtga tggaactaac acagagcaag
    11521 tcacaaatcc taacaaaaca accaatgggt tcttcatata ctctcctcct tagcttttaa
    11581 aaccttgtat ttaagtatat gaacaaccct attaatttat aactttcttc tataaaccaa
    11641 ttattaatct tccactattt cgatttgacc acctaatgct gttatcttct tatctatatc
    11701 aacatagcct ctttggatat ggtatatttc tcctatttga gtttctcctt ctgctataag
    11761 accgcataaa ataagggctg cccctgctct taaatcagta gcgtttactg ctgcaccgtg
    11821 tagctcatct acaccattaa caaccgcact tcttccatct atttttatat tagcacccat
    11881 tctattaaat tcagcaacat gcataaatct gttttcaaaa acagtttcta taactacacc
    11941 agttccatta gctacagtaa gcatagccat aaattgtgct tgaacatctg taggaaatcc
    12001 tggatgtggt aaagttttta tatctattgg ttttaatact tttggaccta caactcttac
    12061 agaattgtcc atttctgtta tttcacaacc agcttctctt aacttagcta caactggttt
    12121 taaatgctcc attaaaacat tttcaacagt tatatcacct tttgtcatag ctgctgcaac
    12181 catataagtt gctgcttcta ttctgtctgg aattacatta tgttctgctc cttttaactc
    12241 tttaacacct tttattttta tagtattagt acctgcacct ttaacatctg ctcccatttc
    12301 gttcaagaag tttgctaaat ctactatttc tggctcttct gctgcattct ctattattgt
    12361 agtcccttct gctaaagagg ctgccatcat tatgttttca gttgctccaa cagatgggaa
    12421 atctaaatat aatttatttc ctactaactt ttctgttgct gcttcaacaa aaccatggtc
    12481 catttctatc tttgcaccta aggctttaaa tccttttaaa tgtaaatcta taggtcttgt
    12541 tcctatagca catcctcctg gcatagaaat tttcgtacta ttaaatctag caagcaatgg
    12601 ccccataacc aaaaaagaag ctctcatctt tcttacaagc tcatatggag cttcacaagt
    12661 ttttatatta cttgcatcaa cagtaagtgt attttcttta tattcaactt ctgcccctac
    12721 atgtcttaat aaatctgata taacatgaac atctcttaaa tttggtactc catttaatgt
    12781 tgatttcccg tttgctaata aagtcgctgc tattattggt aaaactgcat tttttgcacc
    12841 atctatttta acactacctt taagtggatt actctttttt actattatct tagccatatt
    12901 ttttgatctc ctcataatta ataatctaat tttataattg gtgtggcaat atatatgagt
    12961 gttcttccgt cgtcctcatt atatttaatt cctatattta aattaatttt ttcccctaaa
    13021 tattctaatt cataatcatt taatacttta ctatatgctg ttatagataa aaagtttacg
    13081 tctttcactt catctatttg cttagcactc atattatata atattttttg taaaatatca
    13141 tcatatttgt tgttttgtaa cctttttgtg tattctccaa caatacaagc agacacatct
    13201 acttgagatg aatatttatt taacacattt tctacgttac tataaataac ctctttatgt
    13261 ttatatactt tgttatccaa tatgtcaact actatgtaac tttctttgtt atttttattt
    13321 attcctttta tagaaatttc tttatcgcta ccttttatag tagcacataa ttgtatttta
    13381 tcgccagacc ctttttgatt ccaatttatt ccttttttat ccaactttag attgttaatt
    13441 atttctataa aaatattatg aaattcttta tttgataaat tatgatttat aacagaatta
    13501 attttaacat tataaaattt aaaatttgct tctgagattt caagagcttt acttaatcta
    13561 ttgtatctag tatttatact tgtatctgca taagatattg tcatccctat aatcattaat
    13621 ataaaaaagc ttatagctaa ttttactctt ttcatagtaa aaaatccccc ttaaatatgt
    13681 ttttctgtat aatagaatta ttaacaaatt taagagagat atactttctc aatatgtatt
    13741 tttatacatc tttgtatttt tcatctaata agtctctttc aaactcttca atctcaattt
    13801 tctctatcct tattctagat ttgccaattt tttatatgta tcaaatacat cttgcactag
    13861 ttcttatata ttctcttctg taatataaat tacttagtct atacttaaat ctataatcta
    13921 taatctataa tctataatct ataatctata atctattcac attttaccag caaaataaac
    13981 agataatact tttttatcaa tttccatagc tccaaacgaa catacctaag tacacttcca
    14041 aaaacacaat ttacacactc ttcctaataa atcaaaaaca cctttgtttt aaacaactct
    14101 atcgtacttt caaaaacttt atttgtgttc tcccacttca attaaatttg aattatcaag
    14161 actccagccc atccatccat cactataaag ggaggtattt tccactccta tgacatttgc
    14221 atatgttaat acttcagcag cacgccatcc acttccacac ataaatataa gctgtttatt
    14281 cacatctata ttttccttgt cccacatttc taaaatctca tatttatttc tcattgtatt
    14341 atcaatatta cgatattctt ctagtgaaac actatcacta cctgaatgtc catataaagc
    14401 tcctggtata cgcccttttt tatcataata tgtatatcca gatacttttc ctatatgttc
    14461 atcccaagta cggttatcta ctagtataaa cttatttttt tcttttagtt tttgtcttaa
    14521 ttcagaggta gtaacaacta attgtgagtt aactggtata tctgctccaa aattagtaca
    14581 tgaatgtttt ggattactag taaattctaa ttcatatcct gctgataacc atgagtttgt
    14641 tccaccattc aaaactctta catcttttac tcctatatat ctcaatataa cagctagtct
    14701 ataagatgcc ataggtgtac tacttgaaac tattacagta tcatcctttg ttaatccata
    14761 gtctaaagca aacttaatta agttatcatc attatcaagc atccatgttg gaggaggttc
    14821 tataatatcg gtatttacat gaacacttgt aggtatatgc ccttttgtat acgattcttc
    14881 tccccaactc gcttcaatca tttttatatt ctttgaatct tcaaatgtct caggaatttt
    14941 tccatctaat atatctttta ttataaaagc aggaactatc atttgataat ttttatatct
    15001 ttccattgga agattttcgt tgtctgccca ttgtttaata tcatatttat atagatattt
    15061 atatcctttt ttagataaat aatctgctac aaccaaagca tcttttccat ttgcatcata
    15121 taatactata tttttatcta aatcaattct tttagttttc aatgcttgtt ctagcacttc
    15181 atcttttcta tctgagtata cacttaacca attagcagaa aaatctactg ctccttttat
    15241 atgtccacct ctttttactc catctaactt ccagccatta taagcatcat ttaagcgagt
    15301 atcaactata acccaagcgt tatcttgcac tttttcttgt aaaacttcag tagatattgc
    15361 cttaagatta acaaaataat cttctttctt ttctttttta aaatcatttg tcataattta
    15421 tactcccaat ctaaaaattt ttttatttaa aatcattttg ctaaaataaa gtaattttct
    15481 tatactctat tagtttcatt atattctttt ataaatagtt ccttatattc ttattttaaa
    15541 ctactcactt tattttatca caaggttact aataccttat gtattttaat ctttctcata
    15601 atctattatt ttttatgtct gatattgact atatgtttat taagtattca ctatatctga
    15661 tatcgactat atgtttatta agtactcact atactttata taaactatat atttattatt
    15721 ccctatacct tatatggtct atatatttat cattttacag atgatttaat atcaatcttt
    15781 ttataaaatt tattatagca aaaacaaaac aaatttaaca tttatctaaa aaactcatcc
    15841 tataaaatac aaataacagg atgagttctt tttaataaat attatctatt taatttttaa
    15901 tctgttttta ttcaactgtt actgatttag ctagatttct tggcttatct acatcgcatc
    15961 ctcttaatac agagacatga taagcaagta attgcattgg tacaactgct aatattggtg
    16021 ataaaatatc atctgaattt ggtatataga ttattttatc agcagctttc tcaacatcct
    16081 tattatggct ttgagctatt gctactacat aagctcctct tgctcttact tcttccatat
    16141 tagaaaccat tttttcaaat aacttttctt gagtagctat ggcaattaca ggagttcctt
    16201 tttctattaa agctattgtt ccatgcttta actctcctgc tgcaaaagct tctgcatgta
    16261 tatatgaaat ctcttttaat ttcaatgaac cttccatcgc taagctatag tctatacctc
    16321 ttcctaaata gaatgcatgt tcagaactaa caactgtttt agcaacttct tttatatatt
    16381 cttcattgtc tagtatttct tgtatttttg atggtatttc tttcatctta gaaatcatac
    16441 tatcataaaa ttctctagtt attgttcctt ttttgatagc aaagtctaaa gctatcatgt
    16501 ataaagaagt tatttgagta gtatatgcct tagttgatgc aactgctact tcaggtcctg
    16561 cccaagtata gaatacatca tctgattctc ttgctataga tgaacctaca acgttagtta
    16621 ttgaaagtat tcttgctcct ttagcctttg aatctcttaa tactgctaga gtatctgctg
    16681 tctcaccaga ttggctaact aatattacta atgaattttc atctacaaac gggtcgctat
    16741 atctaaattc tgatgctata tcagttatta ctggtatatt tacaaatttt tctatggcat
    16801 acttaccaag taatccagca ttatatgctg ttccacaagc cactatataa actttattta
    16861 tcttttctaa gtcttcttta gaaatattta tactgtctaa gactatattt ccattatcat
    16921 caagtcttct ttctaaagtt tctctaactc ctgttggttg ctcatgaatt tcttttgaca
    16981 taaagtagtc atatccacct ttagacgcag cttctacatc ccaagttaca tgaaatactt
    17041 ctttttcaac aagatttcta ttagagtcat aaactgttac attttcgtct tttagatgaa
    17101 ctacttctcc attttctaag aaataaacat ctctagtata ttttaataat gcaggtatat
    17161 ctgatgctat aaagttttca ccttcaccaa cacctactac aagaggacta tcttttctta
    17221 ctgcaactaa ttcatttcca tgttctttac atataacacc taatgcataa gctcctctta
    17281 attttgatat agttttgtat acagcatcta ataaattacc ctcatagtat ttatctacta
    17341 aatgtgctat tacctctgta tctgtttgag attcaaactt aacaccttca gaaattaatt
    17401 cttcttttat ttccatatag ttttctatta tcccattatg aacaactgct attgttttgg
    17461 cttgattaaa atgaggatgt gagtttacat cagatggttc tccatgagtt gcccatcttg
    17521 tatgccctat ccctaaattt ccatcaatag gattcttttc taagtcttct gcaagtacag
    17581 aaagtctacc tttaaacttt ctaatattaa gttcttcttc attagaacta ttaactgcta
    17641 ctcctgctga gtcataacct ctgtactcta gcttagaaag cccttctact ataacttctg
    17701 ctgcctttct acttcctaaa tatccaacta ttccacacat tatttgtgtc ctcctaaaaa
    17761 tattatttat atattttaag agttaggtca tataagttta ctttggcttc cctttgtcca
    17821 taggattgct cctattattt aaagaagtct ttcgatggta aacacaccgc agagcatccg
    17881 ccgacaattc gattaactct gacctcgtca acttagttcc tatattactc aataattatg
    17941 tctataacta agttctggcg cttagaaagg tgtcctcagt atttatatct gagaataccc
    18001 tttaaattac gataattttt cttgtataag attagctaaa tttgttgcta attcttttat
    18061 ttgaccttcc tctttaccct ctaacataac tctaactaat ggctcagttc ctgatggtct
    18121 tatcaaaact ctaccatttc tgtctaaaat agattctatt ctttctatct cagtttttat
    18181 ttctggatat tccatatatt tgttcttatt ttcatttttt attgttgcat ttactaatac
    18241 ttgtgggtat tgactcatta ttgatgcaat ttctgataaa ggtttctttt cttgtaagat
    18301 tatatttgca agtattagag aactaagcac gccatctcca gtagtgttgt aatctaaaaa
    18361 tatcatatgt ccagattgct ctccaccaag attatatcca tttttaacca tatcttctaa
    18421 aacatatctg tctccaactg ctgtagtaga taaatttatt ccattttcct tagcagctat
    18481 tgtcaaccct atattactca taactgtaac caccaaagta tcttgtgcca atttattatt
    18541 tttctttaaa tttaaggcac tcaatatcat tatatggtct ccatcaacaa tttgaccctt
    18601 ttcatttact gcaattaatc tatcagcatc tccatcatat gcaagaccta agtcagcatt
    18661 atgttccaaa acagcccttt gaagttgttc tggatgtgta gagccacatt tatagtttat
    18721 attatttcca tctggtgaac tatttattga aattacactt gctcctagtt catcaaacac
    18781 tattggtgct actttatatg acgcgccatt agcacagtcc aaaactactt ttaatccctt
    18841 aaaatcagtg cttattattg attttaggta gtctacatag tctctttgtg cacaatgttc
    18901 atgtaatttt cttcctacat tttcaccaat tggaagacaa tcaattttgt ctatatcgtc
    18961 tatatattct tctattttca attcaatttc atcatctaat ttatatccat tcttattaaa
    19021 gaatttgatt ccgttatatt caacaggatt gtgagatgca gatataacca caccacaatc
    19081 agcaccatat tttctagtta aatatgccac tgcaggtgtt ggtattattc caacagtaat
    19141 aacatcacat ccaacagaca taagtcctgc tattaaagat gcttccaaca tatcacctga
    19201 tattctagta tccttaccta ctactacttt tactctgtgg tctccttgag ctaagacaaa
    19261 cccaccagct ctaccaagtt tatatgctaa atcacatgtt agttctgtat ttgctactcc
    19321 tctaactcca tctgttccaa aatactttct cactaaagat tctcctttca taattgaaaa
    19381 gcttgtaatt tgtattatat ttttatagtt cataaaatat cattataggc ttaatttcag
    19441 tcataaaaat attttaatac ttatcagcaa aaaagacaat agaaataatt ctattgtctt
    19501 ttttttgaca tctttttctt tattagttag ttgctttaga acatgtagcg tccatacctt
    19561 tttgtaaagc ttctctgttt aatggaacga atttttcttt tctaggtccg aaaacttttt
    19621 tgaatgcttc tagtacagat tctatatcta cagtatcaga tacttttaag taagcaccta
    19681 acattatcat attagcaact ttatcatttc ctaattcagc tgctaattca tttgctggta
    19741 tataataaac atctacatct gttctatcaa ctttttcttt tattaaagaa ctatttacta
    19801 ttatttttcc accaggaata acatcatctt tgaatttttc aaatgctggt atatttaata
    19861 atatagctgc agttgcatca tctgttataa gtggagaacc aactggttca ttagatacag
    19921 ttactgcaca gtttgctgta cctccacgca tttctggtcc ataagatggt aaccaagaaa
    19981 cttcttttcc ttctagcatt cctgcataag taagtaattg tcccatagac ataacacctt
    20041 ggcctccgaa gcctgcacat attactctag ctgtagacat ttacttcacc tcctcaactt
    20101 caacattttt gaaatttcct aatggatagt atggtatcat gttgtctctt aaccattgta
    20161 aagcatcgtt tggagctagt ccccagtttg taggacaagt agacaatact tctactattc
    20221 caaatcctaa tcctgcttgt tgaacttgga atgctttctt tatagctttt ttagcttgtc
    20281 taacatgagc tggagtatca actgctactc tttctacaaa tacagctcct gttaatgtag
    20341 ctaacatttc acttactctt attggataac cttgagtttc agcatttctt cctgattgag
    20401 cagtagtagc tctttgtcca actaatgtag ttggagccat ttgtccacca gtcataccat
    20461 aagttgtatt gtttacaaat atagtagtta ttttttctcc tctagcagct gcatgaacta
    20521 tttcagcagt accaattgaa gctaagtctc catcaccttg ataagtgaat actgtatttt
    20581 caggatgaac tctttttatt cctgtagcag cagctggtgc tctaccatga gcagcttctt
    20641 gagtgtcaca gttaaaatat ttatatccta aaactgaaca tccaactgga acaacaccta
    20701 cagcatcacc taatactcct agctcttcta atacttcacc aactaatcta tggattatac
    20761 catgtgtaca tcctggacag tagtgagttt gagtatcttg taatccttca gttttcttaa
    20821 atacaacagc cattatctag cacctcctgc aaccaattct ccggcaataa tttttttagc
    20881 tttttcaact atttctgctg gtgttggagt cattcctcct ggtcttccat ggaagtaaac
    20941 tggtaattta ccttctactg ccattttaac atcttccacc atttgtccca tactcatttc
    21001 aacagtcaat atgtttctag cattaggaat ttggttgaat gcttcgaatg ggaatggcca
    21061 taatacttta ggtcttataa gacctgcttt tattccttct tctcttaata tatcaattgc
    21121 gctttttact actcttgaag tagttccata agctgcaaat acaaattcag catcttctgt
    21181 tttgtacatt tcgtattgta cttcattttt ttccattgca ttaaattttt cttgtaaatg
    21241 ccaacaatgg tcttctagta cttctggctc taagtataga gagtttatta cattaggttc
    21301 tctttttcct ttagttccaa cagttgccca atctttagga ggcaattctc ttttcttctc
    21361 aggagctcta aattcaactg gctccatcat ttgacctatc ataccatctg caactaccat
    21421 aactggtgat ctgtaataat ctgcaacgtc aaaagcttcc attatcatat caacagcttc
    21481 ttgaactgta gctggtgcga atactggagt tctgtagtct ccatttcccc cacctcttgt
    21541 agacataaag tagtcagctt gtgaaggttg tatacttcca agtcctggac cacctctcat
    21601 tacattaagt acaacacaag gaacttctgc tcctactgca tatgttatac cttcttgctt
    21661 taaagcaact cctggtgaag atgaagaagt cataactctt gctcctgcac ctgctccacc
    21721 ataaaccatg tttatcgcag aaacttctga ttcagcttga acgaacgctc cacctatttt
    21781 aggtaactct cttgataaat attctggtaa ttcactttgt ggagttattg ggtaaccgaa
    21841 gaaatatttg caaccagctt ctatagctgc ttttccaaat gcttcgttac ctttcataag
    21901 tatcttagcc attaaaattc ccccttaaat attaattaaa ttaatctctt tctactgtta
    21961 taactacatc tggacacata gttgcacaac ttgcacatcc tatgcatttt tccatttcaa
    22021 aaacagttgc tgggttatac ccttttttgt ttattacatt tgagtctaat tgtatgattt
    22081 ttactggaca tgcttcaaca cacaatccac aacctttaca acgttcttga ttaaaagata
    22141 cttttccttt agccatttac ctaagcctcc ttaaactttc tatatatttg agctcaaact
    22201 acatccagtc ttctctcata tataatttta taggaaaaat ttctccttct aaatcttttg
    22261 gtaattgttc aacaagattt tctaaacatg caacatatct tataggtata ttacattttt
    22321 cagaaacttc ttttgcaact ttctgacctc ttaaaacatc atcaatggta gtaaacctaa
    22381 taagatgagt attattaatt agacctgtta cctttaattt agaagatttc tctatttctt
    22441 ttatatattg gataacttct tcagaagttt gagttttctc cctatttgca tttattacaa
    22501 aaagcatatc ataatcgcct tcttttaaca aatgactaaa tcttcctata accctagcac
    22561 ctacattatc tccacctaaa tctataacag aattgtatga atggtctacc ataggagaca
    22621 ttacctctgc tgatattgct ggtaaatcta atgttggagc attaattgag ctatctattg
    22681 gttgtatacc taaagatttc attaactctt tcttttctct agttctaaag tatacattta
    22741 ccacatctaa atcggctata gcaactttgc cagaaaccat atctcttaat ttaacaacat
    22801 agttcatcgc aaattcactt ttaccacttc catagtgacc agttatgatt cttattcttt
    22861 tatcattagt tatcataaac ttgagcctct tcttcaccag ttaaaactct aagtccacct
    22921 tgagctaatg caatcatttc atcttcacct ggataaactt ttacatctgc tataaattta
    22981 actctatctg caatcatttc tgtaaacatt tttgaatatg cgattccacc agttaataat
    23041 attgctttta catctccctt aagaactgca gcactagctc ctatttcttt agagatttga
    23101 tatgccatag cttcatatac taatttagct ttttcatcac cagcttcaat tctttcttca
    23161 acttctctag catcattagt gtttaagtat gcaactaatc caccattacc ttttattttc
    23221 tttttaattt catcttgagt atattttcca ctaaagcaca tttttaccaa tgcacctact
    23281 ggtaatccac cacttctttc tggagagaaa ggtccttctc catctaatgc gtttgcaaca
    23341 tctactattt taccattttt atgagctcca acagaaactc ctccacccat gtgtgcaact
    23401 ataagattta tatcttcata tttcttgttt atttctctag catatcttct tgctattgcc
    23461 ttttgattta aagcatgtac tacacttgct ctacttattt caggcatacc agaaattcta
    23521 gcaacatctt ctaattcatc cacaacaaca gggtctacta tgtatgaagg aacatttact
    23581 tcttcaccta tttgttttgc tattattcca cctaggtttg aagcgtgttc tcctaaaact
    23641 cccactttta aatcttcaat catagcagca cttactgaat aagtaccacc ttttataggt
    23701 ttaagaagtc ctcctctacc tactacagca tctaattcag atgtttttac tccaccttct
    23761 tttagagctt cttctattac ttgtttacga aattcaaatt ggtcagacac cttctcatat
    23821 tttcctattt cttctgaaga atgtcttaaa gttttttcaa atactaaatc ctcattatca
    23881 aatacagcta ttttagttga tgtcgaacca ggatttattg ttaagatttt aaatattttg
    23941 ctcatttgcc tatttccccc ttaaaattaa gcctttgctg ccattaaaac acctaaagct
    24001 attgagttta gtttagtttc ttcactgtct gctctagaag ttaatattat tggtgcttta
    24061 gccccaacta taactcctgc attttttgat tttgagaaga ataccaaagc tttatataat
    24121 atgttaccac cttcaatatc tggggctaat aatatatcag ctcttcctgc tacaggatga
    24181 tttatacctt tatgtttagc tgcttctaaa gatactgcat tatcaattgc aaaaggtcca
    24241 ccaaccatac aacctttgat ttctcctctt tcatacattt cttctagttc tttagcttca
    24301 actgtatctt tcatttttgg atttactttt tcttttgcgc atattgcagc aacttttggt
    24361 tctcttatat ctaatgaatg tgctactgtg caagcatttt ctatgatttg ctttttagta
    24421 tttgtatcag gagctaagtt catagctgcg tcagttacga aaaataatct atcgtatccc
    24481 tctacatcaa atactgctac gtgacttaat acatttccag ttctaagacc tacttcttta
    24541 tttaaaactg cttttagtat tattgatgtg tctactaagc ctttcattac catgtcggct
    24601 tttccttgtg aaactaattc aacagatttt agagatgctt ctgctaaatc ttttatatct
    24661 atcagttcat aattttcgat atccatgtct atgctttttg caatttcttt agtcttttct
    24721 atatctccta ctaaaatggc atttgctatt ttttcttttc tagccatttc aactgccatt
    24781 aaaacttctt tatcttggca acatgctact gatatagttt taggtcctct ttcttttgca
    24841 aacttaatta cttcttcaaa acttctcatg catttccacc tcatcttgaa ttttttgaaa
    24901 tagaactttt tatatctcaa ttaaattata actcgttcag caacgttttc ctagagataa
    24961 ttgaaatatt taattcaaaa tatttattta aatctattta tcagtctttt ttcatatatc
    25021 tagcgctttt tagatttaac aaatatctca ttgtccttat tttataatat aactaaaata
    25081 aacaagatgc aacaaaaaca taaaattaac aaacttaatt ttataggttt ttttgtcaca
    25141 tcttgtaaca actaaatatt cgaaatattt gttcattcca cttcaacaat attaggttct
    25201 atatttattt ttttaaattg atatgttgtt tcatatttta ttttatattt actatcttcc
    25261 tcaggacctt gtgataaatc aatatataat gaaatatctt ttttatttaa cttactcaaa
    25321 tcctccgttg actctacaac aacttttata tctacatcgg aaattacatt tggattatcc
    25381 atatttgttg ggatatttct taattcaata tctcctgcat tatataaaaa ttctgcactc
    25441 aattgttttg tatcatttaa tttaattgtt atagagctat cctcaagagt tataccctct
    25501 ggaatttcta agctaacttc tttttcttca ccattagcca aatcagctaa gtcaataggt
    25561 tttgtcttaa tataatttat tttgtctata tcttctttct ttccttttat tgtaacttgt
    25621 tctttactta atttatagtt tttcagtaca tcattatctt tattatcatt tagacttact
    25681 cttattggga caattttttc cttcaatagt tctatctttg cagtcacaaa agatgaactt
    25741 aatgttactc cttcaacctt agttccatca ctatttaatg gtattaattg caatttttta
    25801 gaaaaatcat ctgtctcatt accaacatct agattagctc ttacactttt gacttcttta
    25861 actagagttc ttggacctga tacatcgaca aattccttac taagttcaat tttatttata
    25921 tttctttttg aatttccact tacttctacc tttatatctc ttttttcttt tatgtttttt
    25981 tcaagattta ctactaaaac atcatgctta aattctagag taacaccttg aggaatattt
    26041 gcttttaaat atacttcctt ctgtccttgt attgggtcct caatggttcc atatacatgt
    26101 atatcttctt ttttaacact ttgtaccttt gataaactac cttttatata tacatctgct
    26161 gttaattttt cgtctggata tataacaaga tttttatcct ttaaatcact catattactt
    26221 atagatacag gtatatcttc aaacccttta gtttcttcag ggtcaactac agccataaca
    26281 tacatccata atgcaattgc acttaaaaat gatattattt ttattttggt attatttttt
    26341 agtctattta tcatttaaat ataccacctt tgaaaaaact tcttgtttct gtatttgttt
    26401 ttaaattact acgtaatata ttcatcattc tttctctaga aatatctcta tataacttac
    26461 ctgctttagc aattgatacg ccacctgttt cctctgatac aatcagagtt atacagtcag
    26521 acacctcact tacccctatt cctgctctat gtctagtacc taaatcttta cttaaatcct
    26581 tactttctgt caatggcaaa aaacatgcag ctgcttttac cttagaatct ctaattacaa
    26641 cagctccatc gtgcaatggt gtatttggta taaatatatt tattaatagt tgcctagaaa
    26701 tttcagcatc aataatagtt cctgtattta ttatatcact tattctagtt tctctctcca
    26761 ttatgataag agcccctatt ttttgtctag aaagtgaata taaagcttct acaatttctt
    26821 ctatatttct attgagagtt tcttcagaag tatttacatt ctttccaaat aaattaaact
    26881 ttgctcttcc tatatgttca agtcctgctc taaattctgg ttgaaaaatt ataagagcag
    26941 ctataactcc ataatctaaa gccttaagag atatccaata aacggtatgc aatttaaagg
    27001 tgttactaag ttgagttgct aataaaagaa atattatacc tttaaacact tgttctgctc
    27061 ttgtatcttt tataaacata aatattttgt aaaaaatata cgctactata gatatgtcaa
    27121 ttaaatcata tatactcatt cttaatatta tattaaaaaa tccatttaaa aaagaattta
    27181 tatctaacac agttttatcc tcctgtttga taacttctta attatttaca ctaagtatta
    27241 tactatataa atctaactct atacaaacta tttaactttt atgtaaataa ttctagtttt
    27301 atatttagag attgttaaat aatctctaaa cacattaaaa atatcaactt aataaataca
    27361 tatttcttat taaagttaat ttttacttaa ataattcaaa ccatcaaggc gcgaatacaa
    27421 aatagtactc gcgccttaat gcaattttat gctctaaaat atcttaatat atctttgttt
    27481 aatatgtatt agttattata aattctataa tccacctatc aactataaac agatacaaaa
    27541 tttaaatatt acttatcatc aatattgttc cattagggtc atttttatat taaagtaata
    27601 acttatttca tatgtagatt agataaattt attttaata
//
LOCUS       DAEMAE010000027        27628 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEMAE010000027 DAEMAE010000000
VERSION     DAEMAE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.027"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(67..558)
                     /locus_tag="KPH93_000953"
     CDS             complement(67..558)
                     /locus_tag="KPH93_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687787.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDKSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(569..1012)
                     /locus_tag="KPH93_000954"
     CDS             complement(569..1012)
                     /locus_tag="KPH93_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687788.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(1002..1841)
                     /locus_tag="KPH93_000955"
     CDS             complement(1002..1841)
                     /locus_tag="KPH93_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687789.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLNLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(2060..3445)
                     /locus_tag="KPH93_000956"
     CDS             complement(2060..3445)
                     /locus_tag="KPH93_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1687790.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDLNTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLVGVQKYNEDDYYFADRLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYIPII"
     gene            complement(3618..4361)
                     /locus_tag="KPH93_000957"
     CDS             complement(3618..4361)
                     /locus_tag="KPH93_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG1687791.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGVDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGEPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(4381..5643)
                     /locus_tag="KPH93_000958"
     CDS             complement(4381..5643)
                     /locus_tag="KPH93_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1687792.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQVMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYCATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMIRANGDLWLKDNKPNINVYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEMPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILIYASLILNL"
     gene            complement(5826..6824)
                     /locus_tag="KPH93_000959"
     CDS             complement(5826..6824)
                     /locus_tag="KPH93_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG1687793.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSAFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEGARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(6808..8319)
                     /locus_tag="KPH93_000960"
     CDS             complement(6808..8319)
                     /locus_tag="KPH93_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG1687794.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVPSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDVSFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLKTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(8373..9335)
                     /locus_tag="KPH93_000961"
     CDS             complement(8373..9335)
                     /locus_tag="KPH93_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG1687795.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMKGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(9383..10273)
                     /gene="rbsK"
                     /locus_tag="KPH93_000962"
     CDS             complement(9383..10273)
                     /gene="rbsK"
                     /locus_tag="KPH93_000962"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG1687796.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGKDGLGEELIRNFRANKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRVKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLIITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTSVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(10315..11328)
                     /locus_tag="KPH93_000963"
     CDS             complement(10315..11328)
                     /locus_tag="KPH93_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1687797.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTLLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(11554..12534)
                     /gene="bioB"
                     /locus_tag="KPH93_000964"
     CDS             complement(11554..12534)
                     /gene="bioB"
                     /locus_tag="KPH93_000964"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG1687798.1"
                     /translation="MEKYTIKLKNKVLRGKEISYEEALNLISLDTNNKNDFDTLLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNDILNIYEGLRKNTNLKLCASLGIIDYEKAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFELY"
     gene            complement(12740..13003)
                     /locus_tag="KPH93_000965"
     CDS             complement(12740..13003)
                     /locus_tag="KPH93_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687799.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(13073..14146)
                     /locus_tag="KPH93_000966"
     CDS             complement(13073..14146)
                     /locus_tag="KPH93_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG1687800.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDKAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENIKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(14615..15373)
                     /locus_tag="KPH93_000967"
     CDS             complement(14615..15373)
                     /locus_tag="KPH93_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687801.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLVATVILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVIPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            complement(15370..16287)
                     /locus_tag="KPH93_000968"
     CDS             complement(15370..16287)
                     /locus_tag="KPH93_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687802.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPFEYLSLCGE
                     ITGLSKNEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(16287..16487)
                     /locus_tag="KPH93_000969"
     CDS             complement(16287..16487)
                     /locus_tag="KPH93_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG1687803.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(16492..17586)
                     /locus_tag="KPH93_000970"
     CDS             complement(16492..17586)
                     /locus_tag="KPH93_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687804.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKSFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPIINESILLAQGQMMNGQIDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSAYLTQVQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     DAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(18098..18238)
                     /locus_tag="KPH93_000971"
     CDS             complement(18098..18238)
                     /locus_tag="KPH93_000971"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG1687805.1"
                     /translation="MNGEGCILSNKITKECYKIGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(18549..19856)
                     /locus_tag="KPH93_000972"
     CDS             complement(18549..19856)
                     /locus_tag="KPH93_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1687806.1"
                     /translation="MDINRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMECIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVGGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIALNNIIDRFKK
                     VFCNGWKIGVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(19921..20400)
                     /locus_tag="KPH93_000973"
     CDS             complement(19921..20400)
                     /locus_tag="KPH93_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG1687807.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGVNPDNIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGIKLEFAQKCAHDDEVCELVFTRK"
     gene            complement(20447..21310)
                     /locus_tag="KPH93_000974"
     CDS             complement(20447..21310)
                     /locus_tag="KPH93_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1687808.1"
                     /translation="MQEIKNKEVKNGQSPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(21310..22065)
                     /locus_tag="KPH93_000975"
     CDS             complement(21310..22065)
                     /locus_tag="KPH93_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1687809.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(22092..22580)
                     /locus_tag="KPH93_000976"
     CDS             complement(22092..22580)
                     /locus_tag="KPH93_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1687810.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(22598..22999)
                     /locus_tag="KPH93_000977"
     CDS             complement(22598..22999)
                     /locus_tag="KPH93_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG1687811.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(23002..23499)
                     /locus_tag="KPH93_000978"
     CDS             complement(23002..23499)
                     /locus_tag="KPH93_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1687812.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNHNINRY
                     KGEFI"
     gene            complement(23523..23927)
                     /locus_tag="KPH93_000979"
     CDS             complement(23523..23927)
                     /locus_tag="KPH93_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1687813.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(24157..26724)
                     /locus_tag="KPH93_000980"
     CDS             complement(24157..26724)
                     /locus_tag="KPH93_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1687814.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLKSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGILFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESIRIGAKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKNFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDEDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVAYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            26890..>27628
                     /locus_tag="KPH93_000981"
     CDS             26890..>27628
                     /locus_tag="KPH93_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG1687815.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINSINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTN"
BASE COUNT         8716 a         5222 c         2803 g        10887 t
ORIGIN      
        1 ttcatatata taatttacac ttatccaaac tacaatttca ttaaatttaa atataaaaat
       61 caactattat tttttaaata gcatctttct taacctatta ttgtaataac ctataaaaga
      121 actgtaaaca tagtcatata ctataaaggc tatctcaaat gaacctataa taaatatgtt
      181 tattggaata taaactattg ttcttaataa aaaatacaat ataagtatca ttatatttgc
      241 aaatactaat ttcataaaat actcaatata tatagactta tctttttcta taagatattt
      301 tacaatacca tatattccaa aggtaagtga atatacaatc cattgagact tacttgccat
      361 cactataaat cctaaaacta tagaacctat ataaaaagca attccactct taaagcccca
      421 ttccattatt acaattgata ctaaaagtga tgctaacccc ataataaaca atgtatttac
      481 aggaattata tttgtaagag tcaacaatat tacatttaat gctaataaaa ctcctccata
      541 agcaactttt atactcatat ttatcatcct aacaacaagt tattaaatct ccaccaaaac
      601 attcacaaca tgaatctaaa caccaaagtt tagcacaatt ttcacaacaa tcgtcaccta
      661 gacaacaaca gcaatcatca cagcaacagc aacaacatcc atctaaatca ttacgtcttc
      721 ttctattata gttgtcagcc ctattgctat aatcatttct atattgtgta taacttctaa
      781 gtgcatcact atattcacta ttttcaggct ccataaattt tgctcttttt aagaagtctt
      841 caccttcttc ataatatcct atgttcattg cagaaaagcc agttaaataa taccactctg
      901 cacatttgtc agttaaagtt tttaaaaaat catatgcttt tttaaattca ctattttcta
      961 tgaatgctct tgctctcata aaattttcat tattataagt attactgtac attctttcca
     1021 cctctacttt ctaatatatt aatatatctt aaataaacac cagaataaat tatattttct
     1081 attattccaa catttttatt tagatttaga ttatcaattc ccctagctaa aaaccctaat
     1141 gatgttacta ttattttttt tactttttct tttaactcat catttttatc aatatactct
     1201 ataaatggat tatatcttcc tttcttaaaa tcttcatcta aatcttcata ggcatctagt
     1261 agatatatat acttacctat attaaaacct atcattctta agtttctctc attttcatct
     1321 tttttataga caaatacttc acccattata tttccaaata tactagatac ttcatctata
     1381 tttatatttt tgtctttttc taacttattt aattcattta gttgttgttt tatatactct
     1441 gctttttcag gatacttttc atatgctaaa tttaatttgt ttttatatat ataatatgcc
     1501 aatttatctt ttatgcgttt atcatccatt aaattatctt ccaatttata atacgttaaa
     1561 agaatattca tacttgctgc atattctgta atatcatttg taattacctt tttcttttta
     1621 aatggattta ctatacacac ttcctctgtg cttatagact ctgggttata tactgctgtt
     1681 aataaaacaa ttaaaaatgt tatatcataa ttcaaagaaa acctagagat ttctccatga
     1741 tttcttttta agtatttaca tagtccacaa tagtaagctt tgtaataatc atattctcta
     1801 aatgttaaat ccattttgtt tattttaaca tatccaaaca taaatactcc tttttgtact
     1861 attttttatt tagattatat atagtataac actttttatc aagattatat aatttacagt
     1921 attctaagaa acttaaaaaa ttccttctaa tcattgtatc ttaaatttaa aataattaga
     1981 aggttgggtt aaaaataata ttaaattata tacttatatt aactcataca agttagttat
     2041 gcttatatca atttaagttt taaataattg gtatatattc aaaactacct acagtatcct
     2101 tcaattcctt ttttgcttta tttataacca attcatcaag cattacatca tttattgaac
     2161 atgatataga tttagatgcc aataacattg ctttaaatcc catatcagaa cctgcactgg
     2221 cacaagattg ccatgtatgg gcagaaatac caataggcca aactgaacaa gaacattgtg
     2281 ctgtaggaat tatgtagcta acatctccaa tatccaatga taatgatata cagttattat
     2341 gagtaaatgt atcatctgta acctcatcat gtaaattcat cttagtaata tccttatttc
     2401 cacttaatac actagaaaca ctacttcttt tatctataga cactgtctca gctaatctat
     2461 ctgcaaaata gtagtcatct tcattatatt tttgcacacc tactaatttc atattttgtg
     2521 atagaatatc tgttaatatt gtatttggaa tatagtcata tactccactt ataatgtcat
     2581 attccatact agtaccagtc attaatgctg ctccttttgc tatatcaaca atcctaccaa
     2641 atacatctct tacatgctcc attttaactc ctcttatgta ataccacact tccgcaaaat
     2701 ctggaactat atttggcatt tttccaccat tagtagttac atagtgtact ctacagttat
     2761 caaatatatg ttctctcaaa taattacaac ctatattcat aagttctact gcatcaagtg
     2821 cacttcttcc gttataagga gcttgtgctg catgagcact aatcccatta tatctaaatt
     2881 tcacagagta attagcgagt gttgggattc ttatcggagt atttagatca aatggatgcc
     2941 aactaaaagc acaatcaatt ccatcaaaaa aacctttttt tatcatcaat acttttccag
     3001 ataaatcttc ctctgctgga catccatagt attttatagt ccctttcagc ttttttagtt
     3061 tgattaattc ctttactgca attacagctc ctattgaacc tgtacccagt aagttatgtc
     3121 cacatccatg acctgcacca ccatctacaa taggttcttt agtagtggat actttttgag
     3181 ataaaccatc taaagcatca tattctccca aaactgctat aactggtcta ccttctccat
     3241 aactagccac aaatgctgta tccatacctt ctaatccctc tatactaaaa ccatgttttt
     3301 ttaaacattc tttttgtaaa tttgaagcat agtattcttt atactgaagt tctggatttt
     3361 tccatatctt atctgatata tctcttaatt ctccctgtat ctcatctata tattccatca
     3421 aaaacttttt atttaaatta tccatattaa ttatataaac ctccctgttt ctaataactt
     3481 atcgattatc agctaaaatg actaatttta actgatttaa aaatattttc taccatacaa
     3541 ccattttata caataaaacc tcttgacttg taaatttcat tttatcaagt aagaggtttt
     3601 caagttttat aaaattttta gcatgttaac caatcaagca tattaaccca taatttatta
     3661 tagtatttcc aatcagtaaa ttcttttggg ccccagtgag gtgcacagtc agaactaaag
     3721 atagcagatt ttccttttcc aaactcgcca acagctacaa atggttctcc tccaatagtt
     3781 gctaaaacag gacaattatc tctagctact gttttgttat atcctaaaaa ctttggccat
     3841 tctgttggta taccttttaa tactggatgt tcagagtcaa taacttctgg aattattcct
     3901 gctggttttt caactctatc atctttatct atcattgtta ttggtaatac atctttgata
     3961 gcagtttctc caaatctagc ttttgcatct actcctgtaa atgacatata tccacctacc
     4021 atgattaacg ccccaccatt atttacatac tctttgatta gttcaagtct atctggatta
     4081 ctattgcaat ctataaaagt actattagat aataagaatg tattagaacc tatatcagat
     4141 aatattatac agtcatactc atttaactct tctaatttat atgggaaact atctgctgct
     4201 acatgtgcag gcatatgaac tgtctcatat cctccagatt ctattgcttc tttcaaccac
     4261 tttacagctt cttcataatg agttgtaaca aatgtatcaa atccttttat atgagttata
     4321 ttttttaccc aagattcccc tactattaat acttttttca tttatatatt cctcctactt
     4381 ttataagttt aatattagac ttgcatatat aagtatacta tctaaatact ccttaatttc
     4441 aacatattca tcaattgaat gacattgttc tattgaacct ggtcctaata taagtgttgg
     4501 catttcagct aaatttttca ttaatctcgc atcattgcca gctgttgaac cttttatttc
     4561 taatttttta cctagagttt cttctagtat tttatgtgca ttcactacaa aatcagaatc
     4621 cttgtccatc tcaaaaccta accctgattg atatacgttt atatttggtt tattatcttt
     4681 aagccataaa tctccattag cccttatcat tatgacattt gttacatcat ttacaacttg
     4741 ttcataactc attatatttg gcaagaagtg tacacataag ttaaatacac acttatctgg
     4801 cacagtagag cctgctgttc caccatttat tacacctaaa tttattgtag gtgaaggtaa
     4861 tagtggatgc ttatatatca taagccagtt atgttccaac tcttttatat cttgaagcaa
     4921 caacatagcc ttctcaattg catttacacc ctcccactta ctaccacagt gaagagacac
     4981 acctttaacc tcaacttcaa agaatacaaa tcccatatgt gcaactatta agttttgttc
     5041 tgatggttca caaattatgc aacaatctcc atcaatacca ttcataactg catttatggt
     5101 accatttccg ccaccttctt catctactac tgacattatt tttacattac caggtacatt
     5161 tagaccacta tcttttataa gctttacagc taatattgat gctataagcc cagatttcat
     5221 atcagcagtt ccaagaccat aaagttttcc attatcttct gtagcacaat atggattata
     5281 tttccactta cttatatcac ctggtggcat agtgtctaca tgaccattaa atacaattgt
     5341 cttaccaggt aaatcatcac tatacttaca aattaaatta tatctatctt gataattatg
     5401 acctagattt ccttctttat atatattttt tgctttttta attaattctt cgctcattac
     5461 ttgtctatct acactaaaac ctatgtaatt tgcaagtttt tcaatatatt cctgtccttc
     5521 tttttcaaat ccacctagta ttccatgacc gacatcttca gtctttatgc ttacaagttc
     5581 ttttaaataa tctatatatt cttgcttttt actatttaat gtatctaaga gaattttttt
     5641 catatttcct cctaagattt atttttacat ttaaaatttc aagtctaatc tcatctattt
     5701 aagccatagt aaactttttc acttagcata ttcccattta atgtataaag atatatgtaa
     5761 attaaagtat aaaaatatat acaaaactta ataattttta tagtttataa gctaaaattt
     5821 aattactatt tagatttagc tgattttttt aattttatat tatttatcca tatagcaaat
     5881 aacattacca aaccttttat aatcatttga ttatatgaag atacacttaa taagtttaat
     5941 ccatttccta atattcctat tattccaaca cctatacatg tccctactat agaaccagag
     6001 ccacccatca tattagtgcc accaagcaca acggctgcta tagcatccaa ttctgctcca
     6061 tctccaacag ttggtgaacc tgatgccaaa cgtgatgata atattatccc agctaatgct
     6121 gttagtagtc cagctagtgc gtatactttt actataactt tatttacttt tactccacat
     6181 aatctagcac cctcttcatt tccacctact gcatgtacat gacgtccaaa attagttctt
     6241 tttaatagaa aatgtcctgc tattacaact ataatcataa ttataactgg tattggtatt
     6301 ggtccaatat atcctctacc gagcatctta aatgcatctg tatttactga tataggtctt
     6361 cctcctgtaa atacaaagct tgccccactt attatcgtta acatggctaa agttacaatg
     6421 aatggaggta gtttttgtgt tgaaataatt gcacctttta tgactcctaa tattgtccct
     6481 attgctaatg caagtatcat acctacccat ataggtgcat tataattcac tactgttgta
     6541 gcccaaaatg tacctactaa agcaattact gctcctacac ttaaatctat tcctcctgta
     6601 attattacaa atgtcatacc tactgcaagt attgcattta ttgatatttg tctcccaaca
     6661 ttaattaaat tatctaaagt taaaaagtta gatgaagcta cactcattat tataaaaatt
     6721 attacaagtc ctacagctgc actcccttgg tctctaaatg cacttgttaa ttttgtcaat
     6781 aatggagaag atttttcttc tttatgctta gttattgaac tcattaattt tacctccgaa
     6841 agccaatttt aatacattat tttcaaccat ttcttcacct tctaaaaatc cacttatatg
     6901 accttctctc ataaccatta ttctgtcact cattgatatt aattctggta tttcagaaga
     6961 tatcattatt atacttacac cactctccac taaatctccc attagattgt atatctctgc
     7021 cttagcattt acatctatac ctctggttgg ttcatccata attaatatat ctggttgtaa
     7081 taaaagccat cttgctatta tagctttttg ttggtttcca ccacttaaac tttgtataac
     7141 ttgattcata tgtggtgttt ttatctttaa agcttctatg tatttttcta aaatctcttt
     7201 ttcctttttt ctatctataa ttgatttaaa cttagatatc ttttttaacc cagccatagt
     7261 tgtgttttct ttgacactca tttgtaaaaa caatccagta ccttttctat cttctgttat
     7321 taatcctagt ccattttcga ttgcatctga tggagtttta aactttactt cttcattgtt
     7381 taaatatatt ttcccagaat catatgtttt aagtccaaat atagcttcta ctatttcact
     7441 tctcttactt ccaactagtc cacctattcc caatatttca ccttttctta tattaaagct
     7501 aacatcatta aaaacattgt tcttagttag attttccact cgcataattt cttcttttat
     7561 ttctactttt cttttcggaa aaaattggtc tagctttcta tctatcatta aattaactaa
     7621 ttcttcctca gatggtacat ctttggtatt caaagtcgaa atcgttttcc catctcgcat
     7681 tatagttatt ctgtcacaaa tctcaaagag ttcttccatc ctatgggata tatatattat
     7741 actgactcca ctctctttta aatcatttat aagtctaaaa agtatttcag tctctttttt
     7801 gcttattgaa gaagtaggtt catccattat taatacttta gtttctgatg ataaaactct
     7861 tgctatttct accatttgtt tttgagctat actaagctct ttgactaaac tatttggatt
     7921 tatatctatt cccatattat cgaaaatctt tttagcctct tcattcattt ttttgtcatt
     7981 ggttaagact ttgccattta tttcttttcc cataaaaata ttttgtgcta ctgttaattc
     8041 gtcaaaaact gttagttctt gatatatggc atttatacct atctttttag agatatctgg
     8101 ggatatattc tctatttctt tgccttcaaa aataaatttt ccagaagttt tcgaatgtgc
     8161 accagttaaa atctttatca ttgttgattt accagcaccg ttttcaccca gtagtgcatg
     8221 cacttcgcca tgaaataact caaaattaac attatctaat gctctgacac caggaaattc
     8281 tttagcaata ttacttaact tcagaattat attactcata atgacactcc tttttaaata
     8341 ggatttattt caaataatct cattttattt tactaattct taaagtcttt tatattttcc
     8401 ttatcataaa gtaaagctgg tactggtata ttcttttcta ccttttcccc ttttatagct
     8461 tttactactg cttctacagc taaacgtccc atctctttag gctgttgttg aatcatagca
     8521 agaatagttc catctgttat tccacttgat gcttcttcag ttaagtcaaa ccctacaatc
     8581 ttaacatcct tattttttgc agattcaagt gcagctttag ctcctagtac actattttca
     8641 gctgttgcat atattatatc tacatctggg ttagattgaa ttaaattttc tactgtatta
     8701 agagattctt ctctgtcata tcctggttgt gaatttaata tcttaacatt tgctgaacct
     8761 tttaacgcat ctttaaatcc atctattctc tgcatttgta tttgagattt taaatctgtc
     8821 actattgcta tttctgactt tccacctaac ttttcatcta tgtatttttt agtgtattcc
     8881 cctaattttt taccagcatc ataactaact gttcctacat aacttgtaat attttcagta
     8941 tttaatacag catctactgt aactactggc attttttcaa atgctccttc tactgctggt
     9001 actatagccg agttatcagt tccaagtact atcattccat ctacattttg ttgtataaaa
     9061 tcttctattg ctgatgtttg cttagctaca tcttggtctg ggtcatttac tatgactttc
     9121 acacctaatt ctttagcttt ttcttttatt ccctcatcaa tatctataaa aaattgatgt
     9181 tgaagagtta ttgtagatac tccaatagtt aaatctttct tgtctgaacc acctttatta
     9241 tctgatttac ttggtccttt cattgaacac cctgttaaca ttcctattgc tagtattcca
     9301 gcaattccta aagataaaat ttttctcact ctcataatta acctccattt ttaatagttt
     9361 taaatttgaa caaattagat ttttatttat tatttaaata tttttcaaca tcttctttat
     9421 aaggcatacc agattgtgca cctaatttag ttacagaaag acctgatact actactgcat
     9481 attctactga tgtatataaa tttttccctt ctgttatagc cactgccaat gctccattaa
     9541 atgtatctcc tgctcctgtg gtatcttgaa catcaacact tatacaagat acatgcttga
     9601 tttcctcacc atcataaaat ctagctccat tttttccttc tgttattata agtttatttg
     9661 gatatttttt taatacttct tctatccctt catttgtgtc aaaaactatc ttatattcat
     9721 gttcatttgg tgttaagtaa gttacttttt ctattatatc ttcatttagt tttactgctg
     9781 gtgctggatt taataaaacc ttaaccctat tttcaaaaca gaaattcact acataattta
     9841 ttgtttttaa tggaatctct aattgtagca atactatatc tgcattcttt atctcttctt
     9901 cattcttttt aattaattct atatctacta gctcattcgt ccctggtaca acaacaatac
     9961 tattatcatt ttcacataag gttataacag caacaccaga acttttgtga ttatttctgg
    10021 atactaagct ataatcaact ttattggctc taaaatttct tattaactcc tctcctaaac
    10081 catctttccc tatgcatgat atcattttta catttgctcc tagtttagaa gctgagatag
    10141 cttgattagc cccttttcct ccaggtgatg taaaaaaact attacctaat actgtttctc
    10201 ctttttctgg tcttttatct acagaacata ccatgtccat gtttacactt cctatgacaa
    10261 ctatgtttcc cattacatta ctcctttaca tatctattta tgtctctcgt gctgttattc
    10321 actttctttt gttgaatctc ttatgactaa ttctggtttg aaaatcaatt catcaaacga
    10381 ttctttttta tctcctctta aacaatttat caatatctca ctggctttat atcctatatc
    10441 ataagatggc tgttttactg tggtaagaga agggcttatt agacttgatg tttgaatatc
    10501 atcaaatccc acaacactta catcatttgg tatacttaat cctctttctt taagacctct
    10561 tatgacccct attgcaatca aatcatttgc acaaaatatt gcatcaaaat ctatcttttc
    10621 taaatttttt ataaattctt gtccccattc aatcttatat tttccttcaa atacataagc
    10681 aggattgtag cttaaattac actcgattag agccttttta tatccttcta atctatctac
    10741 tgctatttca ttttgaagtg gaccacttag gtatattatt ttcttatttc caatatctaa
    10801 taaatgtttt gtagctatat aggctccttc aaagttattt attttaacaa ttccttttaa
    10861 ggtttctttg ttcatgctta tcttcttatc aatcaacaaa gttggtattt ttatatcttt
    10921 ataatttgct tctttccagt ttgtatttga agatgctgca aatataattc catctatcat
    10981 cctctctgat agtgttctta ttccttcata ttctttttta gcatcttcat ctgtattaca
    11041 taaaattata ttgtagcctt cttcattagc tttgtcttct acacctcttg atacatctgt
    11101 aaagaatgga tttcgtatat ctggtataag aagacctata gtattagttt ttttagttac
    11161 taaactttgt gctaatttat ttggtacata atttttttct ttcattattt tcaaaatttt
    11221 ttctcttgtt gcctgactta tattttcatc tttattatta atgacttttg acacagttgt
    11281 tttagaaaca ccagccaatt cgccaatttc ttttatagtt atactcaaga agttcacccc
    11341 caaattttag gaaaccgttt tacttaaccg gttacctaga tactatcaca tcttttttcc
    11401 ctttgcaacc attttatata aattttgata tttttccctt ttttcaaaat attgcaactt
    11461 ataattcaat tttaatattt aaattaatct acaaaaaaaa attaatatac ttttataatt
    11521 atctaaaagt atattaatta atttaagtag tatttaataa agttcaaatc cttgctctat
    11581 aatcattttt ttatcatttt caatcccact cccaacagtt gtcaaataat caccaacaat
    11641 tgcagaattt actcctccat taaatccaac tttgtcatat ccctttaatg atagccttcc
    11701 accagcatac cttatgtaca cattaggaat tataaaccta aatactgctg ttgtttttag
    11761 tatttccatt ggttctaata catcataatt ttccattggt gtattcttaa taggatttaa
    11821 aatgtttatt ggaaatgatt ttacatttaa ttctcgtatt tcaaaagcca tttttagtct
    11881 ctgttcttca ctctcattca ttccaattat accgcctaca catacatcta gcccagcttt
    11941 tttagcatct tttatagttt ttattctatc tttatatgtg tgtgttgtac atatattatg
    12001 aaaattatct ctacacgtct caacattgtg atgatatgta gtcacacctg ctgatttcaa
    12061 cattttagct ttctcataat ctataattcc taaagatgca cataacttta aattagtatt
    12121 tttcctcaat ccttcataga tatttaaaat atcattaaac tcctttcctg acataccttt
    12181 accacttgta accaatgaga atctatgcac tcctttactc tccatctctt tagctctatt
    12241 taagatttca ttgtaatcta ataaggaata ttcttctaca ccagttttat aatgagatga
    12301 ctgagcacaa aatttacagt cttcactaca tttgccagat ttagcattca ttatcgtaca
    12361 taaatctgct tttcttccca taaaatattc tcttatttca tttgcagatt ttaatagagt
    12421 atcaaaatcg tttttattat ttgtatcaag acttattaaa ttaagagctt cttcataact
    12481 tatttctttt cctctcagta ctttattttt taattttatt gtgtattttt ccatataccc
    12541 tcctctttgt caacttacta tttttataca gtttacttat tatatatagt attgcatata
    12601 taacatttat tggtcaactt tattttagtc ataagtttac tattctcaaa aaaatactgc
    12661 ttcaaaataa tattagtata tagattatac atattattat taaagcagta ttttttatta
    12721 gaatataatt cttactgatt tataaaacta tccttgtctt aactccatcc aagctttcca
    12781 ttgtaataac ctctaagcct tcatattctc taaatagcca ttttaaatcc ttcatggctt
    12841 gattaacctg actttttgtt agattattta taaaatattc ttttctagtt tcctcatact
    12901 ctttgctagt caaaaagttt cttagaaaat taaacgcaaa tacacttgga atattgttta
    12961 cattagcctt cttgctgttt tccttctcat agtaaatttt cataaatgca tactcctttg
    13021 atacttttag atttctctta caataattat atgcatttaa gtgctactat tcttataatt
    13081 ttattaattc atattcttta cttcccatac ccatttcaca tgcatagtct attattctag
    13141 ttccatttac atgaggatgt tcatgtttga aaacatcatg acccatttcc ttacaaatca
    13201 aatcgtagca tgcttgttca atagctacag ggtctgttga tgccaatatt cctatatcat
    13261 gggcaattgg tctacctgac caaggtacac aatcacaaag tggagttaca ttcataacaa
    13321 aagttatata ccctaccttg tcttttttat ttgatactgc tccataagcg tattctgcca
    13381 tcttttcgac aaacacatct gagtcacttt cccattgtat agttactgct ctagttggac
    13441 agacagttgg acattctcca catccataac atacatcaga atctataact gcttttttat
    13501 ccactatact tatagctttt gtaggacaag aattaacaca ctttccacaa cctacacact
    13561 tcttttcctt tactactgga gtaacatcag aatgttgcat ttgttttcct gctgcacttg
    13621 cacatcccat agctaagttt tttaatgctc ctccaaaacc tgctgcttca tgacctttaa
    13681 aatgactcat tactatcatt gcagaagaat tataaatttc tccacctatt tttacacttt
    13741 caaaatgttt tttatctatt tttatattct cataatttct actatataat ccatctgcaa
    13801 ttattactgg tgcatttaca actgcataag caaatccatt ttctatagca gttgtaagat
    13861 ggtctacaga atttgttcta ctccctgtat acagtgtatt tgtatctgtt aaaaatggct
    13921 ttccttcgca gtctttaacc ttatcaacca cttgtctaac tgccacagga ctcatatatg
    13981 tagtatttcc cttttctcca aaatgaagtt taattgcgac ttggtcattc ttttctatta
    14041 aatccttgaa tcctgcttta tcaaataatc ttcttacatt gtttggtaca tttttatttt
    14101 gcgaatttga gtataaatca caaaaatata cttttgactt ttccatatct acaccccttt
    14161 aaaatattta cttatactaa ttttatcact tttaaaataa ttttttagta tattttacca
    14221 aaattaaaga ttcttatctt tatttaatga aaacaatatt attttagtcc ttcactactt
    14281 tagactctat atactaaata ttgtaaataa ctgacaaaca ttagattata atctatatct
    14341 ctttttttag atagctcctt aaggtaataa cttaattatt ataaagtata gtgaggacga
    14401 ctaaaataaa aaagtaagtg cctcaaaatg aattttttag tttataagtc acttttttgt
    14461 ataaaatcaa atatgtttta taattttaac aataaaaaag aggcttattt ctctattttg
    14521 agacagcctc ttttttactt aaatacattc ttctacaata ctaaacacat ttttccataa
    14581 tacagtgata tttgtattta taataaaaat atccttaaac tgctttttta ttaaacaaaa
    14641 caacagctaa taatagacat actatcgaaa taacaattgt tacaatgatt ggaataataa
    14701 attctggagg tataacttta cctgaaacta aatctatatt ttttgataat atgaaattag
    14761 gcgtataagg tgcaatctgt ttaggaatag aaatcaaact caaaataaca gttgctacta
    14821 atacaagtaa cacacttgca aatgctggtc taaatagtac acacccaaga attaatatgg
    14881 aaatatacat aatactaatt aaccataagt taaatgctga aaatataata tgattaagat
    14941 ttgcagttgg ccaaaaatac attgtatatc cataagtaca taaaaagctc aaccaaaaac
    15001 caattgtcat aataatcact gcaatagaaa atttagacaa tactacagat gaacgtgata
    15061 accctttagt caacataata acgagtgttc ctttagaata ttcgtttgat aaaatattgc
    15121 aaaaaacaat tagagttaaa ctcgttccaa gaccagaaat atttccggaa aactgtgtcc
    15181 aagaatctaa agcagttggt gcagtagtaa cttttaaagt tggggcaaaa tgagcaatca
    15241 agtctggcat aaattttgca gaaagaggac tcattattcc aaatactaaa aataaaatag
    15301 tcaaactaag taatttataa tttcttatgt tctccttaaa ctcttttttt gtaaatgcta
    15361 tgtattctct cactttacta cctccaaaaa taaattttca agtgataatt ctattagttc
    15421 catctttact ggacaaagat tcatttctgc gagtgtagat ataactgttt tttgtattgc
    15481 ttttatatct ttaccatgca aaacaatttc catattagtt tcttctgaat cttttagtaa
    15541 ggatttaata ccatcactag acttaaactt ttgaatttca tcattacttg caaactccag
    15601 taaaagtcta tcctttccgt gcatactttt tatttctgaa agtgtaccgc taagagcaat
    15661 gcttcctttg tttaatatag caacatggtc acagattctt tcaacatctg atagtatgtg
    15721 tgttgaaaat attacagttg ttgagtcttt aattttaagc ataatatcta atatttcttt
    15781 tcgaccaaca gggtcaagtg cacttgttgg ttcatcacaa ataagtaatt tgggacgtga
    15841 aagcaaagct tgagctatac caagtcgttg cttcattcca cgagaaaacc cacctattcg
    15901 tctcttttca ttaccaagcc ccacaagtga taacaactct tcactcctta tctttatttc
    15961 attttttgaa agccctgtaa tttcaccaca aagagaaaga tattcaaatg gtctcatata
    16021 gttgtaaaac tctggaacat caggcagata acctacatga cgattagaac ttgttcttcc
    16081 ataggtcact ttctcaccac aaacatctat agttccacta tctggttcaa gtaacccaag
    16141 taccattttc attgttgtag tcttacctgc accattctta cctacaaacc caaagactga
    16201 cccttctggt acagtaaaat ttagattatc tattatcttt cgattaccaa aaccttttga
    16261 taatccattt attgagacaa tattcatcat tcttcacctc ttcctattgt aaaatacaaa
    16321 attggtccta ttagactaat aaacagtact acaataatcc atattgcctt atttccaatt
    16381 ttataatttg gatgtctcaa tacatgaaca agtgcagtaa ttataagtat taaatctaaa
    16441 ataataacag gaaccaaaag tggtaagtat tccatcaaat tttccataat cttattcctc
    16501 caataaagat aatttttcta taagaaattt gtattctttg ttttcactta atacagaaaa
    16561 cattggattg tttttaatag catcaacaat acctgctttt acgattgtat catcacgtgt
    16621 aatacctgtt ccaatttcag aaatccatct gtcaagttta ttaaaaaagt catcaccatg
    16681 tattataatt gggtatatat cacgtgttgc taaatctaca tattttcgta atgatttaat
    16741 agctccttca ttatccccat gtattaaatt aatttgtacc tgtgttaaat atgcagaaag
    16801 cataatagca ggagataatg tatcaagctc aaatatatct gaaattgcta gaatacgtct
    16861 ttctatttcc ttcatttgta atttatctgc attttgcaaa atacccacta gattttggac
    16921 taatgatata aggttctgat atgccccaag ttgaaaggtt tctcttgctt catcaatttg
    16981 accattcatc atttgcccct gtgcaagaag tatagattca tttataatgg gaaaattgct
    17041 gttttgcaat aaatcaatca cttgtatggg ttgatttaac aatatataac aagttgcctc
    17101 catacttttt gcttgacggc aaatatctat gtcattacaa gtttcctgta tacgaataaa
    17161 tatttctaat gcttcactaa ttaatagttc acgctttttt tcatctacaa tatcatagtg
    17221 attaactatt aaaagaccca tccgaaaaat caaaggaaaa catgaataat atttttttat
    17281 gatttctctt atttcatcca tgacttcatc aaatgattta accgtaaagt ctttgcacag
    17341 tttattatac agtttattaa tatcttcttt catcatttga ggttcataac atattaactc
    17401 atcaacggta atgttgaaat atgtcgcgag ttgtggtaaa aaggttatgt caggatagct
    17461 ttgtccagtt tcccatttag atacagaagc tttagaaacg cctatataat ttgcaagttc
    17521 atcttgtgta attccttttt ctttacgctt taaaatcagc gtttttgcaa tgtttaattc
    17581 attcatgtca acccctcctt atgtataatt atatccttaa acatgtaaag ctaacaagcg
    17641 atttgaagtt gacttttgac gaaaaaatca accacagaga aacaaataaa caatcatata
    17701 aaatatatat ttaaatatat tttaaatttc ttttgtaaaa tatgtaaaat ttgtaaacaa
    17761 aatccatatt atatataaaa ctaatagtta atctatctaa atacagtttt tatttatcac
    17821 gtttttaaat attttgtgta aaacctcttt taatataaac accttatttt catttctcat
    17881 ttcatattct ttttaaaatc ctgaatataa ctcttcaatt ttccatttaa ttcttttata
    17941 aagttattat tcgtatttat tcctctaatt ataacaggca actatcatgt attggtaatt
    18001 gtctattata taaaaatcta taaaagaaaa gcagtcaatc gtttagatta actgcttgta
    18061 catatatctt attaaattgt ataaactata atttttatta ttctccattt tcatcccaag
    18121 tcataaatcc ccatccacta tctggataag tatcaataga cttttctcta atcatataac
    18181 caattttata acactctttt gttattttat tagatagtat acaaccttct ccatttatct
    18241 aattccattc aattaacttt tcttttcaac cttaactttt ataaaatcat ctttttttaa
    18301 taatttcata cttattttct cttcaacatc atcaaatgag ggtgtctcaa aattaacttt
    18361 ttacttcata aggcactctt ttttgtatga agtaaaatat attttcatca tttcaacaat
    18421 aagtaagagg cttatctcta ttttgagaca gccccatttg atggtgttga acatttaaat
    18481 aaagttgaac tcgttttatc tctcttttta agatatccgt acatttgcac ataaagtctc
    18541 ttctcctttt acaattctaa tagttttatt aaagccaagg tatatatctc catattcttt
    18601 cgtatatgat ttattggtac tctttcatct actccatgac caccagtttt atacgctaag
    18661 aacttaggtc caaacgccac catatcttta aaccaacttg catatgtgcc accacccata
    18721 gcaattgtat atccattttc atctgttact tcttcatagg cttctaataa ttttttaagt
    18781 actaagttac tctcatccac ataatgtaaa tctttatgag caataacccc tattttccaa
    18841 ccattacaaa aaaccttttt gaatctatct attatattgt ttaaagctat atttttacca
    18901 taccttatat ctaactcaca tacgattttt tcctcattta cttttaatat tcctaaattt
    18961 aaggtagtgt ttttgaatac ctcatcatat tgattaatcc ccaatgcatc tccataataa
    19021 tcactacaaa ggtattttga aactaattct atatcttctc tatgttgaaa atccatatta
    19081 tcttcaacta tctttattac cattttagat atagcatttt ctcccttttc aggattactt
    19141 gcatgtacag ctactccttt agtagttaac tctgaaataa ttccattaat aatttttgca
    19201 gtacaatgag acggaacaac attttcttta gttcctcccc ctactattat gtaaggttta
    19261 cttttgtcaa ttttaatttc tttttctatc cttatatgaa cccttccttt ttctccatta
    19321 accactggat acatagcatc tggagtaaat cccatacttg gaatttcttc acctttatct
    19381 aagtaatatt tgatacattc catacctgtt tcctcatttg taccaaaaat taatcttatt
    19441 cttttattaa ttgtaggata catatcctct atgtttttca atacatgtat aagcatagat
    19501 acagccccct tattatctat gacacctctt ccataaataa tattgtcaat tactgctccc
    19561 gaaaatgggt ctacactcca cccctctcct ggtggaacta tatctaaatg cattggaatc
    19621 cctattaatt cctctccctc tccaatttct gcatatccaa tataattatc aatattttta
    19681 acactaaaac ctaaactagc acagtaatct aaagttttat ctaaagattt tttaggtcct
    19741 ataccaaaag gacattgtga aataggttca ccttgttgtg aatcaatttt tatcaattct
    19801 tttagaaatt ctatggttct atttaaacta ttatctacac ttctgtttat atccataaat
    19861 caacctgctt tctatctttt taaagcctgt ccttaaagaa caggctttta gcattaaaaa
    19921 ttattttctt gtaaacacta attcacaaac ttcatcatca tgagcacatt tttgagcaaa
    19981 ctctaattta atgccttgtg cacagtcaac tcttccataa tcaccgtaac aagctaatct
    20041 acaaatttct tttctttctt ctttagttaa tccatattct ttccaagcgg catctaatgg
    20101 acagtgatgg aattttaaaa ctccattctc atctgtattt tctactaatt ccatttcaaa
    20161 cactttttgt cctttactgt tatataacat ttctgccatc tttcctggag tgtctgctac
    20221 gttatatttt tgacctctca ttttaccaaa tccatatatt gcttctttac atatattatc
    20281 tggattaact cctaattttt cagcttcttt cataagaaga tagaaccata ctgctctttc
    20341 tcccgttgct cctctaattc cttctaaata ttccttttct gtatacattt tttctgacat
    20401 ttctttctcc tcctaaaatt aaattctata ttttaatcac tgtaaattag aatattccaa
    20461 tgaatgatcc taccaatgct aatacaacta aaattactaa taacttagtt attgtccact
    20521 tcttgtattt cattccccaa tatacgaaga atacaacacc aagtggtaat aaacctggag
    20581 caatcatatt taatgtatct tgaacaacaa ttgctttacc accaatatca aatgatagcg
    20641 gagttgaaag cttaactgta gttgcagata atgctcccat cataaacatt cctagaattg
    20701 aagcaccatc aattatctta ttgacaatac cacctgatag aattttctta attgaatctc
    20761 tacctaaaga atatcctaaa tggaataagt aataaccttc gcaaaatagt aatatactaa
    20821 acattattgg gaaaaatgca cctataacac ttccttctgc tccaaatgaa gcacctaaag
    20881 ctaagaatat tgtttgaaat gtaccaaaat ctaaagtatc tccaattcca gccaaaggtc
    20941 ccataagacc attcttaaca cctgaaataa cttctcctgg gatttttcct tcagtagctt
    21001 tctcttcttc catagctaaa actgtaccgt gaattaatcc tccccatata gcttgagtat
    21061 taaaaaattg taagtgtctc tttaacgcat ctactaaatc ttcttttctc ttatataact
    21121 tttctaaagc tggagtgaaa cttgcacaaa ctgctaatgc ttgcattctt tcaaaagagt
    21181 tagataattc tgctgtccac caccatctta ggtatgtttt tgttacatct ttctttgtaa
    21241 gtactcttgt cttgttaact tcagttggac tttgtccatt tttgacttct ttatttttta
    21301 tttcttgcat tatacttcac ctaccctttt gtcttctttc ataaaggtaa ttaaaagagc
    21361 tatacaaaca ccaaatattg cagctgcagt aattgatatt tcaaaatatt gaaccataaa
    21421 aaatcctata ataaacattg gtaagaattt attcttacct atagtaaata ttgttgttgc
    21481 aaaacctaat gcaggtaata taccacccat tactgttaat ccattcatta accattgtgg
    21541 tataacatct aataatgatt gaactaaatc tgctccaaaa aagttacata caaaaactat
    21601 tggaaatctt aaaacaaacc caaatattaa agaataaagt gttgcacatc tccagatgcc
    21661 ttttgtatta ccttcttcag catacttatc tgccttatga actaatacag cattgccagt
    21721 tcttcttaag ttattaacta atactcctat aacacctaaa ggtaccgcaa tagatactgc
    21781 aacttctgca ttaacaccag tttgaagtgc tactggtatt gcaataagag cagctagaca
    21841 tttatctgat ggaatatttc cccctgctgc aaccattcct agataaacca tttctatact
    21901 tgcaccaaca atcataccag ttgttacatc accccaaagt aatccaaggc taaaacctat
    21961 aaataaagga gactttagtg ttgagaaaag ggtataccct gcaagacctg ctgcaaacca
    22021 ataccataat cctgttacaa ttgccaacat caaaacagat tccatttata attcctccta
    22081 atttatattt tttatttaaa tttctttaac gcattttcat acgtcataga tgattcctct
    22141 ggtataactt gtaaaactat ttcaacccca ttttcattaa gttcattaag ttgttctaca
    22201 tcctcatttc ctaatgaaac agctgtaaac accattttct ttcctgcttc atttggaata
    22261 cctcctaatt gaatcttttc atatttaagt ccagctttat aagccttatt tgccatatca
    22321 acatttttaa atagaataaa tatattttta tctcctaaag tgttgttttg gaatgcttgt
    22381 ccagcatctt ctgctgttaa aataactact tctactcctg atggtgctgc cattttatat
    22441 atgtcagcca taaattcatc ctgacctaag aagtcatcaa ctattattat tttttgagct
    22501 tgagcgattt ttacccattt tgttataact tgtccatgaa ttaatctgtg gtctactctt
    22561 actaatgtta tttttgccat tatattcacc tcataattta tattttttta aattctttaa
    22621 ttaaatccac acaaaggtta ttattttttt tgacgattct ttctggtaat gtatgtaatt
    22681 tatcttcttt gcttaattct tcatctacga ttaaaattgt ctgtaagcta agtccattta
    22741 ttgcatctat tcctcttctt atagcaacag ctattgcaac acttgatgta gatcctccat
    22801 ataaatctga tattataatt ttcacatctt ctttatctac tacttcttca acctctttca
    22861 taaatacaga gacatctttt tcagaactta gtgataaaac ttcatattga tttgacaatc
    22921 caaagttttt atttgtttct tctactaata cttttcccca agttccatgg cacagtatga
    22981 ttaccttata tttattcatt gctaaataaa ctctccttta tagcgattaa tattgtggtt
    23041 taattctact tcttcttcat caggtaccat ttgaattgat acattaatat tttttttaat
    23101 aaattcatct attaaaagaa actctttttt attaatgtat acattttgag atattttctt
    23161 ttttcctatt cctgtaggta ttcttccaac aataagtttt ttgatattaa tcccttcttc
    23221 tatcattcta tttgcagtat ctaaattttt aattaataca aaaacgttat catcagcttg
    23281 attattataa aaatatctcc ttgattcact tacagttaat attgataaat caatattctt
    23341 tggtgctgcc atttttatca aacctttagc aataacatca ttggttgttt catcatctac
    23401 tactactatc ttttttacac ctagatgctt aacccattta tatatcactt gaccatgtat
    23461 taatctatca tcacatctgc aatattttac taaagacatt tttaatcaaa ctcctttttc
    23521 tattacattg agctaagaac tttattaata ttgtttatac cagtttgtcc tgcttcaata
    23581 catgcttctg ctaactcttc taaacttttt ccctctctat taattaaagc ttcaagtagc
    23641 attggaagat ttactccagt aagtgcatga aactttccac cttttttaag gtttaaactt
    23701 gcaacattac ttggcgaacc tcctaatagg tcagctaata ctaaaactcc gtcaccttca
    23761 tctaattcat ttaacttttc tacaaaattt ttatttaagt cttcaatatt atcattccta
    23821 tttagtttaa ttgctgctgt tctttctgct ggtcctgcaa taagttcaca gctttttata
    23881 atttcttcac taaaactccc atgagtcata actattattc ctatcaccaa tttcacctca
    23941 cttttcttgt cactttttat ataagcaatt gccgtgccaa cttatgtgta tcaataataa
    24001 atggctattt tacaggattt aatcaaacct ttttacttat tttttttaaa aaacttacta
    24061 tttcaatatg tatcaaaagg ttttttcaac ctgtattaaa tctgtatcaa aaaaagtaaa
    24121 aaaaagatag ccttttggct atttttaaat tttgtatcaa cttttgaatt tatctgtatc
    24181 atattgtgat tcaaatatat ctaatatatt tgcatattcc atatctgaaa taattatatc
    24241 aaatgtctct tctactagct taaaactttc ttttataact aaatataatt ctttatttct
    24301 actaattctt tcttcatatg atttataaac taaagactct tttattatta ctctttcaat
    24361 catacatgaa caatgaaata taaactttac taaaagttca tcttgaatta ctaaagaata
    24421 gtgttcacta attctattaa ttacctcttt taatataaaa taagcctttt ccccatttaa
    24481 aaaagtcaaa gttttactaa tattatcaat tagtaatcta tcaaaatatc taccaccatt
    24541 atctttttca gcatatgcaa cttccatatc tccataatgt ttctttatag attctataat
    24601 attaaaatat aatttctcta atgtcatttc tggagttagg gcctttctag ttgcttctat
    24661 tacaattggt gtacttacca tatctattgc ttttacttct tttcctgtag catcacgaat
    24721 aactttagca aatgttaaaa tagaccccat atcagttaaa attaaaattc cctttccttt
    24781 atcaatttta ttagctattt ctacagcttg tactaaaata tcctgaactc tatcttctaa
    24841 tcccatatta attgccactg catggttaca atccagtaaa gtattggcta cattcaccat
    24901 gctagatgct gtagattcac catgcattat gactaatatt gctatactct cctcatttaa
    24961 tcccacatac gataaataaa gaaatgttgc taaaaaaatc gcttcatctt ctggaaactc
    25021 aactccatac attaatgaca tgttagataa tatttctttt gctatgttat attcatatgc
    25081 tctttccttc ataatttcat ctttattagg atgctttact atgtttccaa tctttattct
    25141 ttcaattaaa ttctgaatat gtaaggctat agcttttatt aatttatcta atccttctct
    25201 tttgctccat ttatcgtgct tttgtaaaat atacttcaca gtagttgcaa ttgaatcatt
    25261 aacaatgttg ttataagctg tttgattact actattatat ataaaagtat tcattaaatt
    25321 ataggaatac ttctgtatgt cttcatctat gacacttcta atttgtgact ctttaatacc
    25381 ttcttgttga agtttattcc aagtttcttt tatcattcca taaaaatcta aatcatattt
    25441 tttattttct attaataaat tttcactttc aaaattcttt tcttctggta aaaaaataat
    25501 atcatctttt ccaataaaac tgaaattttg tacaaactcc tcttttctat tatttccata
    25561 taatgattct ctaatattat ttggtagaag agagagcctt acatatacac attccctttt
    25621 ataagtcata taatctaaaa aagctcttgc acaaatcaat tgtatatcgc tttttaattg
    25681 tcctatgttt cccttacact tatataaaat aaatgcctta atgacttctt ttgaaacctt
    25741 taacttagca ccaatcctaa tactctcttc tttaaaaaaa taacatataa gcttcattct
    25801 ctcttgaaaa cctctttctt ctaaagacgg aatctttata gttacaggta ttcttcttaa
    25861 aaacgtttcc aacattgctg tttctgggtc ttctgtagtg gcagcaatta tcataaccct
    25921 acactcgtgt accttattag cctctcctaa ccttctatat atgcctttat ccataagcag
    25981 aaatagcatt tcttgtccct ctgcagataa cctatgaact tcatctaaaa acaatattcc
    26041 attattagct tcatgtacaa taccatactt atctttatca gccccagtaa atgctccttt
    26101 tacatatcca aaaagctgtg ctaataataa ttgctgatta tcagaataat cagcacagtt
    26161 aaatatgata aatggtgcat tatcactatc ttcttttata gttaaactat acttatacat
    26221 atattctgca aatatagatt taccaactcc tgtaggtcct attattaatg tgctcaaccc
    26281 cttaggtgga tatattactg ctgctttagc ctgttctatt tgggatttta aacttccatc
    26341 acatcctact aaattaaaaa tattgttctc tttttttata ctctctaaaa tatactcatc
    26401 tttgatagaa tcattttcac tatattcgtt tatagagtta atattatcaa atatcccttt
    26461 atttagtttc tttcctaaaa tactctcaat actttcttta tataagtact gaacaggctt
    26521 accctttatc tttataataa tactttcttt tattaattga tttaattcct tactagcatt
    26581 acttctattt atacctactt cagtagctat atattctgca tcaaatccaa atctaatgcc
    26641 attattaaag ttcaagtgtt cttctgttaa ccttttcaag caaattaaaa ctttttcttt
    26701 ccttgatagc ttcttttgtg tcatagttaa cctcccctat aagcagtaat actgttaaac
    26761 atttattatt aagcttaatt ctacaatatt ttaatatttt tgtctaatta tgtaactatt
    26821 gattttcttt cctattcgtt ttataatata aattacgtta ccctcatctt ttatagagga
    26881 ggaatttata tgaatttttt atcagattta cacacccact ctatagtcag tggtcatggc
    26941 tatagcactt tactagaaaa tattaattat tgtaaagaaa atggaattaa gatactaggt
    27001 acatctgaac atggtcctaa gatgcctggt tctccccaca gatggtactt tcacaacatt
    27061 caaaatatcc caagaatcat aaataacatt ataattttaa gaggctgtga agcaaatata
    27121 ctagatatta aaggaaacat agacctagag ccctttgtaa ttcctcggct agattatctt
    27181 atcttgtcat ttcacgaagc tgttttttct ccaaatacat tagagaataa tactaaagct
    27241 ttaataaatt caataaataa gcatgataat attgaaatat taggacacct tggaaaccca
    27301 aactacccaa tcgactatga attaatcatt aaattagcta tagaaaagaa tatcttaata
    27361 gaaataaata actgttctat taaaggcgtg tcaagaaatg gtagctcgga taattgtaag
    27421 tatattgcta cactttgcaa aaaatatggt gctaaaatca tactaacttc agatgctcat
    27481 atttgttttg acataggtaa ctatgagtat tctgagaata tcttaaagga aattaatttt
    27541 ccagatgaat taataatgaa ttatcctaaa aaacttatta atcattttca ccaaaaaggt
    27601 aaattactag atgttgatta tacaaata
//
LOCUS       DAEMAE010000028        27389 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEMAE010000028 DAEMAE010000000
VERSION     DAEMAE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.028"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(57..2018)
                     /locus_tag="KPH93_000982"
     CDS             complement(57..2018)
                     /locus_tag="KPH93_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687816.1"
                     /translation="MKKYEIFIFISIIVLCFISFPHKVFGQEENCLEDFERWVEENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHVENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGERAIGVYSKGDVEIEETSIKAYSSNNENGLLKDNNKLVQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGAFSDEIKVSKDDKIENIKF
                     PQYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVI
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAALFYDMPYSASKVFVEYSSDGINWTSEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLREGYSNIVFKPGFIMGGGDDQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDNNNQDDNNQDDNNENENNDSNNNQNNNNENDNSDEKQPN
                     APKDDVESSTGSNENKNPNDIPKNDNKKNNSDKEKGFDNKSSITENDGNYDLNSKSVF
                     GTNEHSFPYRESISNKNQDINKNKSNVSDYDGIINNNYKLESKEYNLKKDIIFLVCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKNNF"
     gene            complement(2202..3677)
                     /gene="spoIVA"
                     /locus_tag="KPH93_000983"
     CDS             complement(2202..3677)
                     /gene="spoIVA"
                     /locus_tag="KPH93_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG1687817.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIMQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3849..5144)
                     /locus_tag="KPH93_000984"
     CDS             complement(3849..5144)
                     /locus_tag="KPH93_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1687818.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGFVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPPKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNSKSNENTTKVS"
     gene            complement(5328..6737)
                     /locus_tag="KPH93_000985"
     CDS             complement(5328..6737)
                     /locus_tag="KPH93_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1687819.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDEIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKHNIPSN
                     AVIIQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLTLWKNYEKKISNNSGR"
     gene            complement(6789..7928)
                     /locus_tag="KPH93_000986"
     CDS             complement(6789..7928)
                     /locus_tag="KPH93_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1687820.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFTIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8247..8897
                     /locus_tag="KPH93_000987"
     CDS             8247..8897
                     /locus_tag="KPH93_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1687821.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(9054..10073)
                     /locus_tag="KPH93_000988"
     CDS             complement(9054..10073)
                     /locus_tag="KPH93_000988"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG1687822.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(10104..10718)
                     /gene="plsY"
                     /locus_tag="KPH93_000989"
     CDS             complement(10104..10718)
                     /gene="plsY"
                     /locus_tag="KPH93_000989"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG1687823.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10753..12072)
                     /gene="der"
                     /locus_tag="KPH93_000990"
     CDS             complement(10753..12072)
                     /gene="der"
                     /locus_tag="KPH93_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG1687824.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(12106..13434)
                     /locus_tag="KPH93_000991"
     CDS             complement(12106..13434)
                     /locus_tag="KPH93_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG1687825.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKRASKTIEQIHQMQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13672..13872)
                     /locus_tag="KPH93_000992"
     CDS             complement(13672..13872)
                     /locus_tag="KPH93_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687826.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13874..14806)
                     /locus_tag="KPH93_000993"
     CDS             complement(13874..14806)
                     /locus_tag="KPH93_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG1687827.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGFYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14851..15783)
                     /locus_tag="KPH93_000994"
     CDS             complement(14851..15783)
                     /locus_tag="KPH93_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG1687828.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15838..17127)
                     /locus_tag="KPH93_000995"
     CDS             complement(15838..17127)
                     /locus_tag="KPH93_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687829.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17156..17848)
                     /locus_tag="KPH93_000996"
     CDS             complement(17156..17848)
                     /locus_tag="KPH93_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687830.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17863..18624)
                     /gene="pgeF"
                     /locus_tag="KPH93_000997"
     CDS             complement(17863..18624)
                     /gene="pgeF"
                     /locus_tag="KPH93_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG1687831.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCKELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18621..19082)
                     /gene="nrdR"
                     /locus_tag="KPH93_000998"
     CDS             complement(18621..19082)
                     /gene="nrdR"
                     /locus_tag="KPH93_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG1687832.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(19104..19367)
                     /locus_tag="KPH93_000999"
     CDS             complement(19104..19367)
                     /locus_tag="KPH93_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG1687833.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19471..20244)
                     /gene="sigG"
                     /locus_tag="KPH93_001000"
     CDS             complement(19471..20244)
                     /gene="sigG"
                     /locus_tag="KPH93_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG1687834.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20336..21058)
                     /gene="sigE"
                     /locus_tag="KPH93_001001"
     CDS             complement(20336..21058)
                     /gene="sigE"
                     /locus_tag="KPH93_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG1687835.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSEREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(21083..21955)
                     /locus_tag="KPH93_001002"
     CDS             complement(21083..21955)
                     /locus_tag="KPH93_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG1687836.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22247..23521)
                     /locus_tag="KPH93_001003"
     CDS             complement(22247..23521)
                     /locus_tag="KPH93_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG1687837.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEVPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23804..24961)
                     /gene="ftsZ"
                     /locus_tag="KPH93_001004"
     CDS             complement(23804..24961)
                     /gene="ftsZ"
                     /locus_tag="KPH93_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG1687838.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25151..25504)
                     /locus_tag="KPH93_001005"
     CDS             complement(25151..25504)
                     /locus_tag="KPH93_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG1687839.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIINNIINKKRKN"
     gene            complement(25603..26277)
                     /locus_tag="KPH93_001006"
     CDS             complement(25603..26277)
                     /locus_tag="KPH93_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG1687840.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(26282..26980)
                     /locus_tag="KPH93_001007"
     CDS             complement(26282..26980)
                     /locus_tag="KPH93_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG1687841.1"
                     /translation="MKKRLINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGSSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(27003..>27389)
                     /locus_tag="KPH93_001008"
     CDS             complement(27003..>27389)
                     /locus_tag="KPH93_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBG1687842.1"
                     /translation="DKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTKEN
                     VFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKILI
                     DLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         8459 a         5056 c         2862 g        11012 t
ORIGIN      
        1 tcataataat gaaaggtatt acttagcaat ctcgtgattt tctaaataat acctttctaa
       61 aaattatttt ttaattttat gaatttaatt atactttttc tagtatcagg atttacaatt
      121 aatatagaac caatcataat tggtgttagt atacaaacta aaaaaataat atcttttttt
      181 agattatatt ctttagactc taatttataa ttattattta ttattccatc ataatcactt
      241 acatttgact tatttttatt tatatcttga tttttattag aaatactttc tctataagga
      301 aaagaatgtt cattggtacc aaatactgat tttgagttta aatcataatt accatcattt
      361 tctgtaattg aacttttatt gtcaaatcct ttttctttat cagaattatt ttttttatta
      421 tcatttttag gtatatcatt aggatttttg ttttcattgc taccagtact tgattctaca
      481 tcatcttttg gagcatttgg ttgtttttca tcagaattat cattttcatt attattgttt
      541 tgattgttat tactatcatt attttcattt tcattgttat catcttgatt gttatcatct
      601 tgattgttat tatcctcatc tctatctggg tcgtctctac cgcctcctcc acggtcacca
      661 tcaatatcat cattttcccg ttcatcatct ggcgtctctt ggtcgtctcc gcctcccatt
      721 ataaatcctg gtttaaaaac tatatttgaa tacccttctc ttaatcctcc taccactttt
      781 actctaaaac aacgcaactt aaaatcttca aaaaaaagta tagcttgatc tctaatatcc
      841 tcctgttctt cagatgtcca attgattcca tcgcttgaat attcaacaaa aaccttactt
      901 gcactatatg gcatatcata aaacaaagca gctacatatc cattttttgt attttgaaat
      961 tccagttcca aattgtctat tggttttttc tctataacag atacattcaa aataggaatc
     1021 acatcatcat ttattataat tttttctttg tttaacattt ctgttttaaa ctttcctgtt
     1081 atattaaaat tttctccctt ttctagcttt tcataataat cagtaaaatc ccattctaca
     1141 tctatatcaa gttgattacc actagatgtt tctaacacca tatattgggg aaattttata
     1201 ttttctattt tatcatcttt acttactttt atttcatcac taaatgcacc aattccccta
     1261 tggcaacatt tcacaatatt atagccagag tcttcagcta tagagggtat aagctcatta
     1321 taatcatcaa ttatataaac tttcttgctt tctgatataa ttgattgtac caacttatta
     1381 ttatctttta gtaacccatt ttcattgttg ctactatatg cttttattga ggtttcttct
     1441 atctcaacat cacctttaga ataaacacct atagcacgct ctccattcac ttcaatatcc
     1501 ttatttagta ttctaatatc attttcagta taaattccta ttgcatcttt tccatttgcc
     1561 tttatttttt caacagattc gtaggatgat ttaatatgta agtctcctcc ttcttcaacg
     1621 tataaggctg tcccatcagt agcaattatg ttatttatac caatacttat acttccacca
     1681 ttttctacat gtattactcc ttcttttcct ccttcaccta ttatattcaa ttcattatca
     1741 tcaataataa atctcccatg attttttatc aatattttat atttgttggt atctattgta
     1801 aaattactgt cataaggaat ataaaatcta aactcttcat ctattaccat atcacaagaa
     1861 agagtataaa ctatttcttc tttattattt ttattttctt caacccatct ttcaaaatct
     1921 tcaagacaat tttcctcttg tccaaaaact ttatgtggaa aacttataaa acataaaact
     1981 attatactta taaaaataaa tatctcatac tttttcatct tacactccta gaaatttttt
     2041 agctttgaaa caatcctgtc gaaacatata cttcacccac tataattttt ccacaattat
     2101 caattattgt caatttatat aagtaccttt attaattgtt ataaataaaa agctacttac
     2161 taaagtaaac agacgtttca ctttaataag tagctatatt tttataacaa aatagttata
     2221 atattagaac ttccttcatt tacaattttt tgtaaagtct tttgcatttt aactcttatc
     2281 tcttctggca tagtatataa tttactttgt aattgctctt ttactaaatc atgtagtgac
     2341 ttaccaaaca tatttgattc ccataaatct gctggttgct cttcaaatac ttccataaga
     2401 tattttatca tctcttcacc ttgattttga tttccaacta ttggagaaac ttctgtagat
     2461 atatctgctt ttataatatg aagtgatggt gcattggctt ttaacttgat accatattgt
     2521 tttccttgtt tcattatttc tggttcttcc aggctaagtt cttctaaaga aggagctact
     2581 actccatatc cttttatctt ggcatctatt aaagcacttt ctattttatc atactcattt
     2641 ttaactttag atagtctagt tatcagacta agcagttgat aatctccttc aattttaaat
     2701 ccactttttt cttcaaggac attatagaat aaatcttgct ttgtctgcaa atcaatattt
     2761 ataacacctt ctcctagttc tacattatca acacctgtat cttccaaaaa ctctaactca
     2821 gaaaatcctt gcataatacc ttctatatct cttattttac ctatatctat aatactttgt
     2881 tttaatgttg ttattatgct actttttatc caatgatttc tttcaagacc ttcaacccat
     2941 tttggtaagt taattcttat ttctgtaagt ggaaaatcat ataatacagc ttccataacc
     3001 tcctctatat cttcttcttc catttcaacc acattcattg gaagaactgg aacttcatat
     3061 ttttcttcca attcacttct aagcatactt gtttcctcac tttttggact taacgtattt
     3121 aaaaccactg caaatggttt tttaagattt ttcaattctt gtataactct ctcttctggt
     3181 tctacatagc tttttctgtc aattccagta actgagccat ctgtcaagac aactatccct
     3241 attgtagaat ggtcttttat aactttttta gttcctatct ctgctgcctt ttcaaatgtc
     3301 atagcttctt gtgaccaagg agtggatact aatctttgtt ttccaccttc ctcatgccct
     3361 aaagctcctt caactatgta tccaacacaa tccaccattc ttacttttaa agatactgtg
     3421 tcttttattt ttatttctac gccatctgct ggtacaaatt ttggttctac tgtcattatc
     3481 gttttacctg aaccactttg gggtatctca tctcttgttc tatccttttt aaattcattg
     3541 tctatattag gtataaccaa cttttccata aattttctta taaaagttga ttttcctgtt
     3601 cttacaggtc caactactcc aatatatata tccccctgag tccttttgga tatatcttcg
     3661 tatatgttat tattcatatt attcctccta tacatctaat cttattaaat tatatttaga
     3721 gttaagggtt aatattctta aaatagaaaa ataaagacca gcaatattaa tttaatttac
     3781 atatagaaat ttgtaaattt caatgtaaat taaattaata ttgctggtcc aaatagtagt
     3841 gtattatatt aactgacttt agtagtattt tcatttgact tactattcat ctcaataagt
     3901 tctttacgtc tttcttttgt catattcatc catataaatg taaacaccaa tccaattatg
     3961 gctactatag taagaactat gaagtagtac ctatatccag ttacacccgg aaatgcatcc
     4021 aatatcttcc ctgctgctaa aggacaaagt acttctggaa gatatcccag tgtagcaact
     4081 attcctatag cagttcccga tattgataat gaatagttac cttcttcaag taacgaatag
     4141 tgtaagcctt gaattgcata catagataca tatattgcaa cacatccaat caatagtata
     4201 taaatcatag atgattttgg tggtgtaaat attacaccta aaagacctgc aatcataagt
     4261 ataaatccat aagatataat cttcgatgaa ccaactttat ctccaagtaa tccagaagca
     4321 gctgatgcta ctggtctaca atactgagct aatattgaga ttgcagatgc aaacaccaca
     4381 gtagctccaa atgcttcagt tgaataaggc gtaaaataat aaaatgacat attagctgaa
     4441 tatgaacaaa acattattat tgttattatc catgtatgag gcatttttat tactgcaaaa
     4501 aaggcttctt tatcaaattt tgctgatacc tctttttcat tatcctttaa tttgaaaaat
     4561 actaatactc caactattac atttattgcc gagtataata ctataatagc tgtaagacca
     4621 gatttatcac ttacttttga tgaaatatac ccaaatagta tcaatactag agatagatga
     4681 attgcattgg taattccacg accactttcc ataaatccaa aagcttttcc ttgttcattt
     4741 tcagatgcta acattctaac agcttttaca agagctggcc aaaaggtaag tattgttgtt
     4801 attccccata gagcatggat taaaaacaca acgatatatg gaggataaag tgttaataca
     4861 aaaccagata ttcctgtaat aattaaagaa cctgaaagca attttctggc tgaaatacgg
     4921 tctgctaaaa aaccaccaaa aaggtatgat accattgcaa gtagaccata aatgcttcct
     4981 aagcttccca tttgtgtatt tgtaagatta aatgcttgtg caaaagtatc ataataataa
     5041 ttctttaaat atggtaaagc atatataaat gctcctgcta caaagagtat tatcaagact
     5101 ttgaaatttt tccaaatgct tggtttattt acttcaactg acatacttcc tccttatgta
     5161 aatacccgta aattttcatg ataattaatt attgcttatt ttattgttta ttcaatattt
     5221 cagaatattt attaatatat taggttaatt tatcaataat ttatggtgag ttatgagcta
     5281 aaagtaaact cttttagctc accataataa ttatattttt atttatacta acgtccagag
     5341 ttattagata tctttttttc atagttcttc catagcgtta aacctgttat tataacaaaa
     5401 agacctcctc ctatcatttc aatctcatat attatattat cttttaaagt ctccataact
     5461 ggtgccgttg agaaaagtat accaactaca gttactacaa gtaccattct ggcaatattg
     5521 atacaaactg aatcactttt tgcaattgaa tatggtcttt cttcgtttgg tcttgtcttt
     5581 ctaagtctaa tataagatgt aaaaagcaag acgtatggaa ataatgctgt taatgctgtc
     5641 atagtaacaa gaacattata tatgttatct actgatggta ataagtttgt tcctacaaga
     5701 agtagactca ctattatagc ctgaattatt actgcatttg atggtatatt gtgtttattt
     5761 gtaacagtca attgcttagg aaatacacct tctcttacat ttccaaaaag catctttact
     5821 ggtcctgcta tataaagtat taatgctcca agtactgata aagttattcc aaatgctact
     5881 attcttataa accaacttcc tatacctaaa tcttgtgcaa ctttagctaa tgcatctagt
     5941 atccctgtag atgctgcaat ttcatctgga gggagtatca ttgttattgc tactgaacct
     6001 aatatataga ttcctcctat taaaacagcc acagttaata tagctttagg aaaatttttc
     6061 ttagcattat ccatttcagt tatgaaatta gcagtcgttt cagctcctga taatgcaaat
     6121 attatagcag atatagaaac taatgaattg gcatctattt ttggtattat attttggatt
     6181 gtatatatcg atgctgatgg agcttttttg agaataacta cagacataaa tgccatcact
     6241 ataagtaata tagcaggaat tgtagaacct aatgctcctg tattcgtaaa aaattttcca
     6301 aatgccatcc cacgagtact tactaaggac aacagccaaa atattatgag tgaaagtgct
     6361 aaaataaaca tcttattgtt tgataaatct ggatttccaa gcatatatgc aatatttata
     6421 gctaaaaatg tcaagaaaga agaataccaa aatatctttg atgtccaatt tagccatgac
     6481 accataaatc cccacttctc tccataagct tgtttaaccc attctccaag tccaccatct
     6541 tttggatatg ttgcagccaa ctctgcacat ataagtgatg caggaacaaa gaatataaaa
     6601 gcaaacaata accaaactgg tatagaacca agacccaatc caaaactaga ggcagtagat
     6661 ttagctatcc atctaatccc aaatagtgct gatatattca taaatactaa atcccaaaat
     6721 ccaattttct ttttcactgt aattcctcca atttcaaatt tattcaaact catacacaat
     6781 gtattgattt aaatatcaag tataagttct tcaacacttc ttggataatt agttataatt
     6841 gtagcaccat tcttacctac tattgctgta tctgagtgtc taaatcctcc aactccaggt
     6901 atatatatcc ctggctccat agaaaatacc attccctctt ctaatattaa ttcattgtca
     6961 aatcttaaat atggctcttc atgttctgat aaacctaaac catgaccaat tctatgattt
     7021 gaatataact ctagaccata ctttgcaaca acagctcttg ctgcttcatc caccattctt
     7081 gctggtatac cagcttttat tgtatctaat ccagcttgtt gagcctctac tgcaatctta
     7141 aacacttctt tttgtctttc tgttggttta cctatgataa aagttctttc attttctgct
     7201 ctatagtttt cataccaaac ctgtctacta tgtattacta tatctcctct ttgaagtatt
     7261 ctagtattgg aatctaaatg aggttgcgct gttctatcaa tccctgaaca agtccagttt
     7321 gcaaatccta tatatgtatc aggataattt tcagaaacat attttagtaa agcattatcc
     7381 cctgcaacgt caaactccaa ttcactcatc ccaactctaa cattttctaa agaccctttt
     7441 attcctatat cagataggta acctgctatc ttcaaaaatt ctatttcttt tgcatctttt
     7501 acatatctca tttcaaatat tttacttcca acatctttga tagtaaatga cttatcagtt
     7561 atatactctg aaaaacttgc agatactata tctttttcta ttccaacagt tgttccttta
     7621 ggatatttat ttaaaattat atctaagtaa tgtttatgtg atattccatg ctctttcatt
     7681 tctggtattt cataatatac atatacatta tcaacttttg caacttcttt agcatgtaat
     7741 tcttctaatc caggtactat taattctatt ttatcttgag ttactactgt tacaattggt
     7801 cgagaataag ttattgcttt aaatccacta atataaaatt ggttgtctgg ttcaaatatt
     7861 aaagcaattt caatgctttt ttcattcatg tactttctca atctttctag tctcatatta
     7921 tttgacataa tgcacctctt tcttaatatt cagataattt attcaataca acaaggatga
     7981 atatattatt ttaaaattct taatattttt attacttgcc gtatttgtat gtaaattaat
     8041 tttatttgtg attattttat cataatcatg attattttgt caatcacgat tttattttaa
     8101 aagatttttt atattctagt gattttaaat tatctaaaat ataacgagta tacattgtat
     8161 tttggaatat tattcttgat ttatgataag tttaatgata aaatcacgat tatataaact
     8221 atattgatta tttgtaggtg aataaaatga caaataaaga attacaaaag aaaaggatat
     8281 tgatgtattt catagaagct gctcaaaata ttatggaaaa cgaaggtata gaaaatatta
     8341 cacttcgtaa ggttgcagat atggctggct ataatagttc aactctatac aaatacttta
     8401 aaaatttaga ccatttaata tcatttgcat ctataaaata ttttaaagat tataacttga
     8461 atatttcaag atgtatagaa aatgtcaatg atgaatataa gaaatatata attatgtgga
     8521 aattattttg taaacattct tttgataatg ctcaagcttt ttatcaggta ttttttaact
     8581 tgtctagtga tgaacttagc tatataacaa aacaatatta tgatatgttt ccagaagatt
     8641 taggtattca tgatagtgac atatctttaa tgattacagg agaaagtata aaggaaagaa
     8701 ataagatact attaaacaat cttgtagata ctggatatat accagctaaa gatttagaca
     8761 taatcaatga tataattatt tctttttatc aaaatctatt attcttaaaa aagaacacag
     8821 gaaaaaattc tgatgatgat gaattaacta tgaaaatgat ttcttctatt gaatacataa
     8881 taaaacatgc taaataaaat taaaatatgg ctgtctcaaa atagagataa gcctttttta
     8941 ttgttgaaat aataaaaata tatttaattt catacaaaaa agagtgcctc ttaaactaaa
     9001 aagttcattt tgagacaccc ctaaaacaaa tttattattt attatacaat agtttatata
     9061 tcatcaccta aaactacttc ttccatttca tgctttttac ttctcatcat tagttctatt
     9121 ccaacttctt taggatttga acctttcttt attacagaat atatagcatt agttataggc
     9181 atatctatat ctaatttttc agcaacatca tgtgctactt cagtagcagt tataccttct
     9241 acaaccatct ttacttcttt taaagtatct tctaaactca tacctttacc tatgagtata
     9301 ccagctcttc tatttctact atgcatgctt gtacaggtaa ctattaaatc tccaatacct
     9361 gaaagacctg caaaggtaga catattagct cccatagcaa ttccaagtct gctcatttca
     9421 ctaatacctc ttgtcataag ggcagctttt gcattatcac cataccctaa tccgtcacat
     9481 atcccagctc caaatgctat aatatttttt aatgctcctc caagttctac tccaactatg
     9541 tctggattcg tgtaaactct aagtttaggg ctcataaata aatcttgtat catctgtgct
     9601 atttttaaat ctttagaagc aactactacg gtagtaggaa tatcccttgc tacttcttca
     9661 gcatgagatg gcccagatag tattacatat ggattttgtg gtaattcttc ttcacagact
     9721 tgagataatc taagcccagt ccctttttca agacctttag ctacattaac aataacttgt
     9781 ccttctttta taaatggttt tattttttgg caagtacttc ttacagcctg tgatggaact
     9841 gctaaaacta ttaccttaca atcttttata gttttttcta tatcagtact tatagttata
     9901 ttgtttggaa ataatactcc tggcaaatag tctgtatttt ctttagttct gtttattttt
     9961 ttagcttgtt cctcttttcg agtccacatg ctcacatcat tacctttttt tgcaagccct
    10021 aaagctaatg cacttcccca gcttccagta cctaatacac atactttctc catgtttaac
    10081 accacctgag tataatatat ttactattct ttcttttgac caatttttct ctcggtccca
    10141 tttaataatc gctttatatt tgctctgtga ttatatatta ctgatgcagt taagaacaga
    10201 gctactatta aaccagcttt attttttacc ataatcataa atattggtga acatcctatt
    10261 cctacaacag aacccaatga aacatacttt gttatagcta ccaccaatat aaatactgct
    10321 agacacatta gtgttattac tggatttaca gctagcattg cccctagtga agttgcaaca
    10381 cctttacctc ctctaaatcc taaaactgct ggccaattat gacctgctac aacacatata
    10441 actgccagat atccagctaa aagagtatct attccaacca atcttgctgc aaattctgat
    10501 attaaaacag ccattacacc tttaagaaca tcacctaaaa atgtcattgc tcctgctctt
    10561 tttccaagtg tcctaagtac atttgtcgaa cctgcattac cagaaccttg agtccttata
    10621 tcaaccccag caattcgctt tgcaactatg tatgaggtag atatatttcc taaaagatat
    10681 gcaactacag caataattat ataactaaat atctccactg aatttccccc ctttagttat
    10741 attttaagat atttattttt tatttttttg tctatactca aactttatgg aagttccttc
    10801 aaatccaaaa ttttctctta tcttattctc aaggtatctt tgatatgaga agtgagttaa
    10861 gtccttatca tttataaaca atgtaatttt aggaggtcta atgtcagttt gagtcccata
    10921 gtaaattttt aatcttctac ctttgtcaga tggtggttga tttaacatta cagcttctcc
    10981 aattacttca ttcagtgctg aagttgatat tctcttagaa tgttcatttg acacatattc
    11041 aactgtatct agtatcttgt tcattctttg attagttttt gcagaaacaa atacgattgg
    11101 agcatacatc ataaatggaa atttttcttt tatatctttt gtgtaattgc tcatagtctt
    11161 attatctttc tctattaaat cccatttatt tattacaaat atgcaccctt ttccttcatc
    11221 atgtgctata cctgcaacct ttgtatcttg ttctgttaca ccttctaagg catctattac
    11281 tatcaatact acatctgctc tatctacagc actcatagct cttattacac tgtatttttc
    11341 tattgtctca tatattttac tttttcttct aagccctgct gtatctatta gaagaaattt
    11401 ctgtccattt ttttcaaaat aagtatcaat tgcgtctcta gtagttcctg caattggact
    11461 tacaattact ctctcttctc caagtatctt attaagtata gaactttttc ctgcatttgg
    11521 tttacctgtt atagctactc ttattatatc ttcttcatac tctgtatcta atcctgctgg
    11581 aaaattttca actatctcat ctaataagtc tccaaatccc atactattgg ctcctgaaac
    11641 tgcaaaaggc gttccaaatc ccaactcata aaaatcgtat atattatcaa attgactttg
    11701 actatcaatc ttattaacca ctaagattac tggttttttt gtctttctaa gcaattgtgc
    11761 aatttcttta tcagcagctg ttattccagc tttaccatca actataaaaa gtattacatg
    11821 tgacatgtcc attgcaagca tggcttgatt tctcatttga gataatatta tatcttcgct
    11881 atcgggttca attcctcctg tatccactaa tgtaaaatat ttatctaacc actctacttc
    11941 agcaaatatt ctatctcttg taactccagg tgtattttca actattgata tcctttttcc
    12001 tgcaaattta ttaaatattg tagacttacc aacgtttggt ctcccaacta cagctacaac
    12061 tggcctactt atactcattt tatttctcct tccatctttt gttttctatt ttaaaatttt
    12121 atccacaaag tcttttcctt cattcaaaca agggataatt tttattctca taagttcttc
    12181 taattgattt aattcaatat tatctaagaa aatttcttca tctgctttta gcatacttct
    12241 tgttatataa agagcttctc ctaattcttt atcttcaagt tgttctttta aatctttagc
    12301 tgttaaaaga cctgatacag ttattgtctc tccaaaaaac ttgtttttga tttcatatac
    12361 atttacttga agatttttaa acttgtccat catagcatct gccatactct gtataaattc
    12421 atatgcagaa tgaccagtag ctattgatac tttcttctct ctttttagaa tattctcagg
    12481 caatatattt agatactctt caatctctgt ttttaactta cgtatcatac ctacaccatc
    12541 ttcaaactgt aaaaaacctt catattcttc atatccaggc aattcactat tagatagtat
    12601 ataaaattca tctgataaaa atgcaaatcg agtacctaat ttttcaagat atttctgttg
    12661 catttgatgt atttgctcta tagttttact tgctctctta tcattaaata tatttaattc
    12721 tactagattt tctctatgct ttgtaatccc aactggaact atcgcaacac tatttacata
    12781 cggataaagt tgagctagtt cttttattgt tctatccagc tcctttccat catttatacc
    12841 tggacataaa acaatttgac agttcatttc tatatgagca tctgccaatc tcttcattat
    12901 accatataat tttcctgcaa atttattgct tatcattttt tgtcttagct ctggattagt
    12961 tgtatgcaca gatatattta taggacttat tctatatttt ataatattat ttatatcttc
    13021 ttcactcata tttgtaagtg tgacaaaatt tccttgtaaa aaagataatc tcgaatcatc
    13081 atctttaaaa taaagagttt ctctcatacc ttctggcaat tggtctataa aacaaaacat
    13141 acacttattt ctacagcttt tagcttggtc aattatcgga tttgtaaatt caacacccaa
    13201 atcttcatca tagtcttttt ctatctcgta tatgtagact tcgccatctt ttttttgtat
    13261 ttctacatct aaatattcat cacttagtaa gaatctatac tctattatat catgtattgg
    13321 ctgttcattc acagatataa gtaaatctcc tacctctata cctatttctt ctgcaatact
    13381 atctttataa accttactta taatattatt tacttttttt acatttactt ccaatatttt
    13441 caccactctt ttgtattcat caatgcttat tataatatat tttcatatgc aatttattta
    13501 tttattatac tttataatag catttttaag catttcatgt cataataaca cattttaatt
    13561 ctaatagtca atataagtaa atagctataa aatagattaa aacaaataat attcaaattt
    13621 aagtttctta aaaatttgaa tattatttgt tcttaaaaaa attttataaa tttaatgttt
    13681 tactatgatt aacgtcccat ttgtcatatc atgaaccatt ccacctacac actttgacat
    13741 caacaatgcc ttattttgta agtctttctc atcatttgca taaaatattg gacaacctcc
    13801 accatttttc atattcttat caagtgtaac tatagctagg gtatattcat ttaaacctat
    13861 atccattccc atcttatgca cttcctttct ttcctatttt ataatttttt agtgataagt
    13921 tttttccttt agagcttgaa agtattgggc atgactttac agcttccatt actttttcca
    13981 cattcttatc tactggtaaa taagcaatca ttatactttc atcctcttta tttcttctag
    14041 gtaatggtac aaatgaaggt tctccataat ctccatagaa acctaacctt gaatatatat
    14101 tgtatactat tgcttgtctt tgaccggagt caaataatat tcctgcatta gcataatctt
    14161 tatttttagg aattatttgt attcctacac ctctattaat atatacttct ctatcgcttt
    14221 ttagacctat atttgttata ccttttaaat ctcccacctt tagtatagat tcgtccacaa
    14281 aactaatttc agcaggcatt acatcagcta tgtctccaat actttttcta gtaagaagtt
    14341 tttttaatat aaaggctaac actaatccac aaattgaact tattaccaaa ctcattcctt
    14401 cacttatttt cgtttctgca agtattacat aatacaatcc tattgttaaa aaagatgtaa
    14461 ctatgcacat ataatttctt acttcatagg ttctagctat ctcttctatg aatgcagcac
    14521 ctctctttac aagttgaact tcctctaaat tttgcaatgt atttcttcta tttgctctca
    14581 cttgtctaaa ttgttctgct gctagagata aaaatgttat ggatgtataa gaacgattta
    14641 taagagctgg aaccaataat gctcctaatg aagaagcgac gaatgctaat ataagttgag
    14701 ataataaaac atttggttgc gttggatact gtttttggtc taaattcaac attataattc
    14761 tagatgttat accaataata acagcaacaa aaaatactct atccattaaa tcactcctaa
    14821 aatatttaat ttgaaatttt acatgtaaat ctacaaagtc tttctagaaa aatcactctg
    14881 ctttcctttt gatgtttcta tgataggtgt acttttcact acatctatca tgacatctat
    14941 atctttgagt attggcatat atggtataac cacagtactt ttttctaaat tggtcttaga
    15001 tatagcaaca atatcaacct catctacatc tctatctatc cccatatgaa taaatagatt
    15061 atgtattata gcttgtcttt gccctatatc atttactatt ccaaatgcac ctaaatctct
    15121 tggtataact tctatggcca gacattcatt tctatacttt tctcttgtac tttctagacc
    15181 tatattagtt attattacac catttaccat taaaataggt ccttcaaaac ttatttttgc
    15241 aggaactata tctgctatat ctgctatact gtttcttctt aaaaatcttc taaaaatcaa
    15301 accaacaata gctcctgaca caatagctaa gattgtacaa taaaaaaagc taaggtcata
    15361 ctttcttgca aaaacgatat aaactattga agatactagt gcagaaaaaa gacttatata
    15421 acttctagat tcgtacgtag atgctatttc ttcaacgtaa gcatctcctt ttggtactaa
    15481 ttcctcatta tcgatattct ttagtgttat tctttcttgt tccgcaagac cttgaaactg
    15541 ttgtattgct actgcaaaaa atgtcaaagc tgcaaactcc ttatctataa gtgcaggtaa
    15601 tgctatagca cctaacgaag ctgaaagccc agatataatt atctgttcta aataatcttg
    15661 tggtcttgat gggtattgtt tgtctgtaac tcttaaaact aatcctctac ataatattcc
    15721 tacaactaat gcaactataa aagagtgtct aaataaattt gcattcaata aactttcttc
    15781 cattttctca gctccttctc ttatatagtt tgtgcaaaaa acaaaaaaaa ttcccttcta
    15841 tttaaggaaa tttttttgta atattgtaac tataaattca ctaccaatgc caagttcact
    15901 ttttactttt atacatccat ttaacgattt gactatatgc tttgttatag ccaatcctaa
    15961 acccgttcct cctacatccc tacttcttgc tttatctacc ctatagaatc gttcaaatat
    16021 gcgttctaca tcttcttttg gtatccctac accattgtct ataactttaa ttacgatttt
    16081 ttcttcatct tttaacacta caacttgtac aatcccatta tcttgggtat actttatagc
    16141 attatcaact aggtttaaga acacttgctt tatatagtct ttattggata gtatacttat
    16201 tgatttatca tcacatttat ataaaacttc tatatttttt tcactagctg aatgttgagt
    16261 tatttcatat acctctttaa atacatcata tactacaaca ttttccataa gaagagtatc
    16321 tttattttct acaaatgata aaagcaatat atcatcaatc aaccttttta atctgtctga
    16381 ttcgctttct attatttcta agaatctatt tctagtattt acatctatat tttcatttga
    16441 ctttagtgtt tcgacaaatc cacttataga tgtaagtggt gttttaagtt catgacttac
    16501 atttgcaaca aaatcacttc tcatattttc aagtttaact tttttagtaa tatcttctat
    16561 atttactatc gaacctatta ttacgttgtt agtattctga agataaactg ggtctacttt
    16621 tattttatat acgatatcat ctttagtagt aatattagca gtttcattat ctttagaacc
    16681 aataaattga agtatttgtt ttaatatttc ttcttctttt attacaacat taatattatg
    16741 cccttcaact aattgatttg gttcactctt tatcatttct tttgcttcat catttatcag
    16801 aagtatattt ccatttacat ctatagccaa aattccatga gatatactct ttaatataga
    16861 ccttaactgt aaatgtttat actgtacttc ttctaaggtt ttatccatat tctttatcat
    16921 atgattaaaa tttctagcta attcgccaag ttctcctttg gctgatatgt taagcctagc
    16981 atgatattca ttattactta ctttttttga gacgtatata aactctttta aatatttcct
    17041 taatgttatt gtgtatctta cagctaatat aacaatggct atggacaaca taacaataaa
    17101 aaaatatata ctatttaaat cttccacaag ataacactcc taaaatataa cctttttaat
    17161 ctattttgta tcctacacct cttatagtct ctatatattt ttccgattta tcttcatctt
    17221 ctattttttt tcttaaatat cttatatgaa catctactgt tctagtttct ccatagtatt
    17281 cataccccca tattttatcc aaaagataat ttcttgaaag tacttttcct ttattttgaa
    17341 ttaataattt taacaattca aattccttaa gagttaactc tatcttttca gagcctttgg
    17401 taacttcatg ttttgataaa tctagtttta aattacctgt agtaagtata ttgttttctt
    17461 cacctaagct agtcaaatta tatctcctta aaacagaact aatcctagct aacaactctt
    17521 ttatactaaa tggcttagtt atatagtcat cagcacctat ttcaagccct tcaaccttgt
    17581 cattttccat atttttagca gtaagcatta taacaggaat attttttaag tccttatcac
    17641 ttcttatttt tctcaaaact tctattccac ttatatttgg taacatccaa tctaaaagta
    17701 ttaaatctgg tttaatttcc ttagccttta taaacccatc aaatccatca tatgaataac
    17761 taacttcgta atttgacacc tccaaattga attttaacag ttcaactata tgcatttcat
    17821 cgtctataac aagcacttta gtattcatta aaaagctcct ctctatttta tctgtagtac
    17881 cgttcctatt cgttctgatg ccttattatg ttttctataa gagtggaatt tatctgctct
    17941 acaactagtg cataaattta aatttattat attttctttt ttaactccac tacatctaag
    18001 catatattca ttaattttcc ataaatctaa atagtaactt ccctctttta ttatatagaa
    18061 cttttcatca ttatttgtaa gaattgtgtt aaatttttct actaaatctt cagacacttc
    18121 ataacaacag ggtccaattg atggtccaat tatacatact aaatcttcag gaactgtttt
    18181 atagcattca gacattaatt ttattgtttt atgacctata ttactaaaag tacctctcca
    18241 acctgcatga gccagtccta tggctttatt tttagcatct ataattgata taggtacaca
    18301 gtctgctgta aacagtaaaa gtggtacctt ttccaagttt gttataagcg catctccatc
    18361 tcttcttttt cctatagtat ctttattaac tatgttaact atatctgaat gtatttgaga
    18421 attactagtt agattttcaa atgtcaaatc acactcttta catactttta acatatcttc
    18481 tctatttttt gcatctatat tcttgcatgt tataactaga tttacagagt ttaatttttc
    18541 atcaagttct ttacaattac cacttaaatt aacatactct ttaatatctt gtacattttt
    18601 tatatttata taatctttca tcatttgtct cctttttcta tcagtatact tttaagttca
    18661 tttacaaaag tatttatatc tttaaattgt ctatacacag aagcaaatct tacataggca
    18721 acctcatcta actctttcaa tttatccatt accatctcac caattttttt ggtttcaatt
    18781 tcttctataa aacatttatt tatttcattt tcaatatgta caacaatact ttcaatctct
    18841 tcaatagaaa caggtctttt ttcacatgct tttagcaatc catattttat cttttctcta
    18901 tcaaaatatt ctctactatt atccttttta atgaccatta aaggagttgt ttctattttc
    18961 tcatacgtag taaacctctt tttacatgat tcacattcac gtcttcttct gatagatttt
    19021 aaatctgtat gtcttgaatc tattaccttt gattctttat aattacaata aggacattgc
    19081 atctatattc ctcctttgtt taactatatc tcaaactcat ctaagcttaa ttctgaacct
    19141 atatttacta ttattgtatc acaacctatt ttaagtatat cattccaaaa tataacttct
    19201 tcatcccctc ctcttgaaaa aaaacttcta cttccatctc caaaaatggt aaatgagact
    19261 accttacctt cgtgaatatc catgtctatg tctattataa accccatcct ttttccattt
    19321 tttacattta taatttcttt ttccattata tcagaaactc taatcatatt gtactcaccc
    19381 tttctttaaa aataaatatg tatcgaagta aaaaaaaatg acctatatag gtcatttaaa
    19441 ttaaaataat gtttacagtc catggattac ttatacatat tttctcatat tttttaaagc
    19501 attcttttca attcttgata cttgtgcttg agatataccg atttcatcag caacttctat
    19561 ctgagttctt cctttgtaaa accttaaatc taataccaac ttctctctac tatttaattt
    19621 cttaatagct tctttaagtg atatttcttg caaccaattt tcatcagtat cttttttatc
    19681 ttgaacctga tccataacga atattgcatc tccattgtct tgatatactg ggtcaaacag
    19741 agatattggg tcttgtatgg catcaagtgc cattacaact gattctactt ctaattctaa
    19801 ttcctttgct atttctgata ctgtaggttc tttagaattt gttctaatta atctttctct
    19861 aacttgtagt gccttatatg ctatatcttt taatgaccta cttactctaa ttgggttatt
    19921 gtctcttaga tatcttctaa tttctcctat aatcatagga acagcataag ttgaaaacct
    19981 tacattttgg cttaaatcaa agttatctat agcttttata agaccaatac aacctatttg
    20041 aaataaatcg tctatatttt ctcctctgtt gttaaacttt tttattacac tcaagactaa
    20101 tcttaaattc cctcttacaa actgttgtct ggcttcttca tcaccatttt ttatttgtaa
    20161 taaaagttcc ttcatttgct tatttttaag aactggaagt tctgatgtat taacaccaca
    20221 tatttcaacc ttattaactt gcatatattt tcagtcctct ctcaaaagta ttcatctatt
    20281 gaaattattt acatttccaa tttttttata ctttttaaga agaattaatt taacattata
    20341 caaatttttt catttctttt tgcaaccttg agattatttt cttttcaagt ctagatatgt
    20401 aggactgtga tattccaagc attccagcaa cttctttttg tgttttttca atacctttat
    20461 caataagacc aaatcttaat tccataattt gcttttctct ctcacttaat ctgtcaagtg
    20521 ccataaccaa caaatctcta tctatttctt cttcaattat tttgtatatt tcatcatttt
    20581 ctgtgcccaa gacatcagat aataaaagtt catttccatc taaatctata tttaaaggtt
    20641 catcaaatga tacttctgtt ttctttttat tattttttct taagtacatt aatatttcat
    20701 tttctatgca tctcgaagca taagttgcca attttatatt tttattcagt ttaaaagtat
    20761 taactgcttt tatcaaacct atagttccta ttgaaatcaa atcttctaca tctatacctg
    20821 tattttcaaa ttttctcgat atgtagacta ctaatctaag atttctttct attaaaattg
    20881 actttacact ttcatctgtt tctagctttt gtaaaagttc catttcttct tctggtttca
    20941 atggaggagg aagtatattt gcccctccca tatagtatat tccctttggc tttattattt
    21001 ttaatttaat acctaacata gtaataaagc ttattattct ttctttcaat cgtaacattc
    21061 cttgtccccc ttaaattttc acctaaaatc caggcaatat atttggattc attattgctc
    21121 catattctct atcattaaaa tttgaaatac caagtattat atttcctatc ttttgctcat
    21181 ctacttctat atagtcagct tttaagccta ttattatgct tgtcttatta cttccagcat
    21241 gtttataagg tattattcta actctacctg aagttttatc atctaagctt cctataattt
    21301 cttgtgcttt cttcatatct atatcttcat aattaatatt taatagtttt tctggaataa
    21361 atccttctaa cacactagac ttaactatta ttacatcact ttgacttatt gggtctttta
    21421 acaaattacc actatctact aaagctttac agcaaaaact tttttctagt aagtttatat
    21481 ttatagtctt tgtaagctcc ttaatgtact taagcatttt caaatcttta taaatgtatt
    21541 ttaataattc acaactcaca taagtacatg caattaagaa agatattttt aagtgactta
    21601 ttcctgtaaa ataaattata aaataagtgc ttccagaaat aaaaatgttt actagataaa
    21661 aaactaacgc tacatgaaca tagtcttttt tgctcttact tctaaatgaa attaaagtta
    21721 ttatactcat gattattatt ttaaatggta cagtaaataa tatattcatt gatggataca
    21781 aataagcaac agaataggca cctcctaaac tagcccccaa taacttattt tttttaggat
    21841 tatatctttt agtaagaata gaagtacaac ttataataat ataatttatt actaggtttt
    21901 ctataacata gtactcaata tacaccatgt atcccccctt aaaaccttta aacatattat
    21961 aagatagtat gtacaaaatt ttatgtcact tttgatggtc gaatccaaaa caaattctat
    22021 ttaaaaagaa caatgatata tgtcttatac tttatgtcgt caaaagatat ttttttaata
    22081 ccaaacaaat agactttttt gtagaatcca atatatctag gtagaagtta ttcgctctac
    22141 ttttggtata tttacataaa aaaagactac ataatttaat aatatatcaa gtaaatcttt
    22201 tacttatata aaatcaaatt atatagtctg taaattcaaa tataaattat tgtgctagta
    22261 atccatttgc atattttgca gattcattta gtgcttgttc tggagttatg tctccagcta
    22321 tcatcatttg aagttgtttc cataaatatt catatatctc tactccatga ggtccaactg
    22381 tcaatggtct atacatatgt ttatctcttg tcaccatttc ttcaaatctt gggtctcctt
    22441 ggtatttttc acctttagca actggagctc taggtacttc tttgtggaaa gcttccattg
    22501 attctttgct tagcatatat tcaagaagtt taaatccaag ttctttattt ttacatgctg
    22561 acattaatgt taaatcatct actgaagcaa aacttccaac tgtcttatct tctaatgatg
    22621 ttacatatcc ccagtttaaa tccgggaatg atttatcaaa tgtacttgtg gcagcagagg
    22681 atagcattat agtaaatgca gcttttcctg gtccaaatac actttcaatc atttcattac
    22741 tatcttttga taaagcatct tctggaatat actttttcat atcatttata aagtttgcag
    22801 ctttcaagcc ttctgcatta ttaaatttaa ctgattttaa atcatcatta tatatatctc
    22861 caccagcttg ccataaatac ggataccagt tccagtttaa gcttccaaat actttagaac
    22921 cccaaccttg tgcaagaccc cactggtcga tttttccatc tccatcagta tcttttgttg
    22981 ctttttcaca aattctttta aaatcatccc atgtttttgg aggtttttct cctaattttt
    23041 ctagtatatc tttattgtag tacaatacgc ctggattagc agcttctata ggtaaaccat
    23101 aaataccacc catcattttt gcatcatcta agtatagata attatccgaa gttcctgatt
    23161 tttctaaata aggtgttaaa tcttctaccg cacccatttc tataaattga ggaaacatct
    23221 ctgcatacat gtatcctata tctggacctt cgcctgcact tattgctgtt gcatattttt
    23281 ctgcgtagtt atcccatggt actatctgaa attcaatttc acagttattt tctttttcaa
    23341 atttttcaaa tatcggcttc catgcatctg catcatggtc tgttaatggt gccgaccata
    23401 caactaattt ttctttaccc tctttgcctt tagaatcttc tttttttgaa catcctgcaa
    23461 gaattgatac tgatagaatc cctaccataa ctaaagaaag aacctttttt agtttaatca
    23521 tgcttaatcc cctttcagac tttaatttgt ctttttcctc aaatgtttac atttaattca
    23581 gatttaatat taacaaacaa gactactctt gtctaatcat aaaaaactat actaactcct
    23641 ataactatat tttttttcta tacctattag atttgtttat actaaaaact tactttttaa
    23701 attttatcaa tatttaaaaa tatttttaag tctagctaca aactaactat ttaaattgaa
    23761 caaaataaaa agaagtctat tgacttcttt ataagcttac atattatctt cttcttctta
    23821 ggaatgtagg tatctccatg ctctcatcat catcttcttc aattatactt gcctttttag
    23881 gaggctctat tttttcctca gcaattactt cttcttcaat ttcttttaca gcttgtttta
    23941 ctgttgtatt taatgaggaa cgtatacttg atttaggttt agtatctaaa tctaaggcac
    24001 cattttgtaa tccttcaaat cctgttgcga ttactgtaat cattatttca tcacctaaat
    24061 cttcttttat agaagctcca aatataacat ttgcttctgg gtcacaggat tccataacta
    24121 aagttgatgc ttcattaact tcaaataatc ctaagttagg accacctgta acattaagaa
    24181 gcactccttt agctccttgt attgatgttt ctagaagtgg agattgaata gcttgtcttg
    24241 cagcatcaat tgctctagtt tcaccactag cactacctat acccatatga gcaagcccct
    24301 tgtctttcat tatagtagta acgtctgcaa agtccaagtt tattaaccct tcaactgcta
    24361 taaggtctga aattgattgt ataccttgtt ttaatacatc atctgcaacc gcaaaagcat
    24421 ctaacattga ggtatttttt tgtactatct gtaaaagtct atcatttggt atcgttataa
    24481 gagtatctac ttttgattta agttctgcta ttccaccttc agcgttcttc attcttatct
    24541 taccttcaaa tgcaaaaggt ttggtaacta cacctactgt aagtatcccc atttcttttg
    24601 ccaagcctgc aactactgga gcagctcctg taccagtacc accgcccatt ccagctgtta
    24661 caaaaaccat atcagctcct tgaagtaact ttactatttc atctttactc tcctcagcag
    24721 ctctttttcc tacttctgga tttgctcctg ctccaagtcc tctagttaat ttttctccta
    24781 tttgaacttt atattcagct tttgatgtat ataaagcttg tttatctgta tttactgata
    24841 taaattcaac gccttttagt tgagcttcta ccattctatt gacagcattg tttccaccgc
    24901 cacctacacc tattacttta atttgtgcac attcttctaa ttctacgtca aagtttagca
    24961 tcataaaccc ccttctctca tgattcaaag ttatcgtatg tattaattat atcattttct
    25021 tcttttattt tgttaacctt taagtaacat attccacaaa aacgtaaatt ttccttttta
    25081 aatttcttac ttatattgtc aaatattact ctttaagtat aaaataattt acgtatatat
    25141 taaatgctct ttaattttta cgttttttat taataatatt attaataata tatcgtctaa
    25201 ttatagataa gttattaaac aatctcatac caaaaactag tacagcaaca tagtaaagag
    25261 aaactcctaa cttttctcct atataagtca ataatatagc caatatagca ttacctatga
    25321 aaccagtaat aaaaattaca ttatcatagt tatcttcaaa attggatttt atcgctccaa
    25381 aaatagaatc cattattgca agaatagcta ctgacatata tatggaataa tccatagaat
    25441 atgtaaatgg aatatagtaa cctactacta caccaaatgc taatcctcct aaaacccata
    25501 acataaatct tcacctcttt attttacttt gaaacacaac tacattacaa tcctttttat
    25561 taattttttt tactttttct ttattttttt gtatttttcg ttttattttg caaataaatt
    25621 cactttattt ccattatttt cctgaacttt tatattaatt ccatatatat ccttaagaga
    25681 tttagcatat gattctggag agtttatagc tgcactcaat aaatctatat ctcctatagc
    25741 ttttattata aatggttgtc catatgtagt atcatttact gtaatagtag cccctgaaca
    25801 ttttatctta cttgttgcta gtactctttc cccatttata gaaattgctc ttgctccagc
    25861 cttttttaaa tcatttaaaa ctctcaaaat atctatatca tgtataatca aatcatttgg
    25921 attctgcccg tcttttaact ctctctcact atccctcata gtaatagtta tgcctggccc
    25981 tgtaacagtt gagtaaccac ttaattcttt aacttcttca agctcttttt gcataagtgt
    26041 ttggatagat tcatttccat tcttttttgc ttctttatat tcattcaaag tttttttgtt
    26101 gtttttaaga gtttctttta aattttttat ttcttttttt gtattttcta cctcactttc
    26161 aatactttca gtttgaccta atgttatgta tactctattt gggtctagtg attttatata
    26221 agtcgatatg aaaacaccta atatgaatgc tcctaatata actattttct ttttcatact
    26281 attatttccc ctctgctttg tttatatcac tatgaccatt tatttctata ttgtcttctt
    26341 tggtcacttt tactttagca ttataatatg tttctatttc ccagacaata ccatacttta
    26401 tattaagtgc cgaagccaat gtatcagaat caccaattgc ttttattata attggttctt
    26461 ttatgacagt gtcattcata taaagcttgt cttcttttaa atacatatat gtattagaaa
    26521 ctattctctg gtcgtttata gatatggcac ttgcttgtgc tgaatttaat ttatttatca
    26581 ttgacaataa taaatcataa ttatatagta tactatcatt tttatcagag cttcctaagt
    26641 cttttgtaga atttatctca atttttatgc ctgttccttc aacatctgta tatccagcta
    26701 atgtttcata tttttctatt tctgacttaa gtatgtcttg ttctccttca ttgtttttgc
    26761 ctctataagt ctctatatct ttcttcacac tattaacttc tgattgtaaa ttctcttttt
    26821 ctttttttaa agatttaagt tcttctgtaa gttgctctcc tcttttaaaa gttgtcatcc
    26881 ctctattttc tatattaata attttaagtt gtaaactaac aagaacaccc aatacaatac
    26941 ttcctatgat tatactttta tttataagtc tttttttcaa tatatcactt ccttacaatt
    27001 gtctatcctt ctggactata tactgccaac ttcccataat taagatttat agtccctcca
    27061 ttagtatttt tattttgcag gtcaatcaga attttactta ccctagatat attataatct
    27121 aagttatcat cattagaaag taagattttt atattacttc ttgtaagcat ttctatattg
    27181 ctttcttttt ttaagtttac ataatttatt tttctataaa ttccttcttc ttttaaataa
    27241 tttaaagttt taaatacatt ctccttagtt ttataatttt taaattttat agatttatta
    27301 tcatcaacgc tataatctac atctactatc aagtcttttt tgctttcatt agaacctctt
    27361 agttcttcca ataaatttcc ttttttatc
//
LOCUS       DAEMAE010000029        27347 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEMAE010000029 DAEMAE010000000
VERSION     DAEMAE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.029"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..1733
                     /locus_tag="KPH93_001009"
     CDS             39..1733
                     /locus_tag="KPH93_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG1687843.1"
                     /translation="MKFSEFKYERPNYNSIKKEFLGCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTLNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKSVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFYFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKLTHLSSAIKFMPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DSGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2065..2175
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2279..2650
                     /locus_tag="KPH93_001010"
     CDS             2279..2650
                     /locus_tag="KPH93_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG1687844.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2744..3691
                     /gene="trxB"
                     /locus_tag="KPH93_001011"
     CDS             2744..3691
                     /gene="trxB"
                     /locus_tag="KPH93_001011"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG1687845.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3776..4093
                     /locus_tag="KPH93_001012"
     CDS             3776..4093
                     /locus_tag="KPH93_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG1687846.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4373..5659
                     /locus_tag="KPH93_001013"
     CDS             4373..5659
                     /locus_tag="KPH93_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG1687847.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5771..6244
                     /locus_tag="KPH93_001014"
     CDS             5771..6244
                     /locus_tag="KPH93_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5903..5905,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG1687848.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6276..7586
                     /gene="grdB"
                     /locus_tag="KPH93_001015"
     CDS             6276..7586
                     /gene="grdB"
                     /locus_tag="KPH93_001015"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7323..7325,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG1687849.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7843..9375
                     /locus_tag="KPH93_001016"
     CDS             7843..9375
                     /locus_tag="KPH93_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG1687850.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9386..10531
                     /locus_tag="KPH93_001017"
     CDS             9386..10531
                     /locus_tag="KPH93_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG1687851.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10840..11919
                     /locus_tag="KPH93_001018"
     CDS             10840..11919
                     /locus_tag="KPH93_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1687852.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGEASEKQLEIYNLVKEAQNVGVENMKVGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12030..12629
                     /locus_tag="KPH93_001019"
     CDS             12030..12629
                     /locus_tag="KPH93_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687853.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPSRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNVMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICVVALTVL
                     FIVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSRPD"
     gene            complement(12816..13688)
                     /locus_tag="KPH93_001020"
     CDS             complement(12816..13688)
                     /locus_tag="KPH93_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1687854.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERPITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14105..15508
                     /locus_tag="KPH93_001021"
     CDS             14105..15508
                     /locus_tag="KPH93_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687855.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15535..17079
                     /locus_tag="KPH93_001022"
     CDS             15535..17079
                     /locus_tag="KPH93_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG1687856.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVVGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNST
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEDGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGIVITNMQDKTRPLGVVDEASKKISNNIITFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEEDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17110..18501
                     /locus_tag="KPH93_001023"
     CDS             17110..18501
                     /locus_tag="KPH93_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG1687857.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKAQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18696..20192
                     /gene="abfD"
                     /locus_tag="KPH93_001024"
     CDS             18696..20192
                     /gene="abfD"
                     /locus_tag="KPH93_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG1687858.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20327..20602
                     /locus_tag="KPH93_001025"
     CDS             20327..20602
                     /locus_tag="KPH93_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687859.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20626..21933
                     /locus_tag="KPH93_001026"
     CDS             20626..21933
                     /locus_tag="KPH93_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG1687860.1"
                     /translation="MSWRELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPTFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            21953..23071
                     /locus_tag="KPH93_001027"
     CDS             21953..23071
                     /locus_tag="KPH93_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG1687861.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFNRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKIKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23356..24276
                     /locus_tag="KPH93_001028"
     CDS             23356..24276
                     /locus_tag="KPH93_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG1687862.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLADQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TYESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24391..25548
                     /locus_tag="KPH93_001029"
     CDS             24391..25548
                     /locus_tag="KPH93_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG1687863.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVAGFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25633..27132)
                     /locus_tag="KPH93_001030"
     CDS             complement(25633..27132)
                     /locus_tag="KPH93_001030"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG1687864.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT        10664 a         3135 c         5160 g         8388 t
ORIGIN      
        1 taaatacaat aaagtaaagt tgtaaaggag tgctttaaat gaagttttca gagtttaagt
       61 atgaaagacc caattataat agtataaaaa aagagttttt gggttgcgta gaagatataa
      121 ataattctcg taattataaa gagcaacaaa aaaatataca taagattaat ttattaagaa
      181 ataaaataga aacgttgtca aatatagcgt cgataagata tagtacagac acacttaata
      241 aattctacaa agaagaaaaa aattattggg atgaatatat gcctttgtat gaagaattaa
      301 attcacattt ttataatgct attgtaaatt caaaatttaa atatgatttg ataaaagagt
      361 ttggagagca atttttcaca atagtagagt attctttaaa aagtttttct aaagaaataa
      421 taagtgaatt gcaggaagaa aataaattgt gttcagagta tactagactt ttagcttcag
      481 ctgagataat gtttgatggg aacataagaa atttatctgg tatggggaaa ttcatgtatt
      541 ctaagaatag aaaaacaaga gaattagcaa acaaagcata ttataatttc tttgaagaaa
      601 atgaaactaa atttgatgac atatttgata aattggttaa acttagagat aagatatcta
      661 aaaaactagg ttttgaagat tttgtagagc ttgggtacgt tagaatgatg agaagtgact
      721 acagagaaga tatgataaaa agtgttagaa aacaagtatt aaaatatgtt gttccaatgg
      781 ccaatgagct ttatgaaaaa caagctaaaa gaattggcct agaatattta agttacatag
      841 atgaaggcgt ggaattttta acaggaaatg caagtctaaa gggagattca agatacataa
      901 ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga aacaaatgaa tttttttatt
      961 ttatgttaga aaatgaactt atggatttag aaacgaagaa aggaaaagga gctggagggt
     1021 attgtactta tataccagat tataagtcac catttatttt ttctaatttt aatcaaactg
     1081 ctgatgatat agatgtgtta acacatgagg cagggcatgc atttcagttg tatatgtcta
     1141 gatggattga tatgccagaa attaattttc caacgcttga cagttgtgaa atccattcaa
     1201 tgagtatgga atttataaca tggccatgga tggatttatt ttttaaagaa gatacagata
     1261 agtacaaatt aactcactta tcatctgcaa ttaaatttat gccttatgga gtaattgttg
     1321 atgaatttca acactatatt tacaaaaatc ctaatgtaga taagtcaaag cgaaaagaaa
     1381 tctggagatt tttagagaaa aaatacttac ctcatagaaa atatggtgat aattcttttt
     1441 tagagagagg ttgttggtgg tttaaacaag gacatatatt taaaaatccg ttttattata
     1501 tagattatgt attagctcaa atttgtgcgt tacaattttg gaaaaaaatg attcaagata
     1561 gagattcagg atggaaagat tatataaata tctgtaaagt tggaggaaca aaatcatttt
     1621 tagatattgt gagtatggga aatttgtatt ctccatttga tgatggctgt attgagtcta
     1681 ttataggtga tgttaaatct tggtttgatg aaataaatga cagcaaatta taaaaaatta
     1741 tgagaatttt attgtataaa agtgaaaaat taaaaaatac ataaaaagtg attatttgtt
     1801 gtgaaaatag tcttgttttt aaccaaattg ataaaataaa ctaaaaaaga tttgtactta
     1861 aaaaaaaaag aaaatacaag aaatatgtaa tatcaacatt tggaaaaaaa agtaaaaatg
     1921 tatacaaaat acatataaaa aaactattgc aaattttata gaatgtttat atacttaagt
     1981 caacagagat agaaaaaagc tatttataga aaacaaagga taaattgctt aattttaaat
     2041 tttctgaaaa aaataataat ccggatgaag agtatgggag actgctttat aaaaagcgac
     2101 cgaagggaca agtttaatct tacttaccca aaagattaga tgaaattctc aggtaaaggt
     2161 gactgtactt ggacggggct ctgaaaatga ttttaacagg gaagacaaat tgggagattt
     2221 gtttttcctg ttttttattt atcaagtaat aaataaaagt ttgaaagaga gtaaaaaaat
     2281 gataataatt acgaataatc caaaagtaaa agaagaagtt caaggcagag aagttttgtt
     2341 taaagacaca acttatattg gaatcttaga agcaagtaga gatttgatac atgaaggtta
     2401 tgaactttta tctcatccac tctatgggag tgtaaagcca aatgaaacac cttacaggac
     2461 tgttatacta aagaaaggaa atcgtttgga tataaactct ttaaccttaa ttgaagaagc
     2521 aatcatcact gcaagtaaat ttcagaataa taaaaagact ccaaaatgga cagaaagtgt
     2581 tcaagatgat tttagagtaa ttgattatga tatattttat aatacaatac aaagaatgca
     2641 gtatgaataa aaaattgtta aaaagttatt aaaacagctt aaatattaaa tctaatttaa
     2701 tattaaataa aatgagaaat ttaattgggg aggataagtc aaaatggaaa atgtgtatga
     2761 cttagtaata attggttcag gaccagcagg gcttgcagca ggtctttatg gagcaagagc
     2821 aaagttaaaa actctaatcc tagaaaaaga caagactggt ggacaaattg taataacaca
     2881 tgaaattgca aattatccag ggtcagttcc aaatgcaaca ggaccaagtt taatagctag
     2941 aatggttgaa cagtgtaaag agtttggagc agaaatgcta agagacaaca ttgttgatac
     3001 tgagctagat ggagatataa aagttttaaa aggggaaaag gcagaatata gagcaaaagc
     3061 agtaataata ggaacaggag caacaccaag aaagattggt tgtcctggag aaaaagaact
     3121 tactggtaaa ggggtttcat attgtgcaac ttgtgatgca gatttctttg aagattttga
     3181 agtatttgtt gtaggtggag gagacagtgc attagaagaa gcaatgtatt taactaaatt
     3241 tgctagaaaa gtaacaattg tacatagaag acaaggattt agatgtgcta agagtgttga
     3301 agaaaaagca aaagctaatc caaagataga gtttttatta gatacagtaa ttgaagaaat
     3361 caaaggtgat ggaatattag aatcagttgt atttaaaaat aaagttactg gagaaactca
     3421 tgaatacttt gcagatgaag aagatggaac aatgggagta tttgtgtttg ttggattaga
     3481 tgctcaaact gatttattca aaggtaaagt tgatatggat gaaaaaggtt acataataac
     3541 tgatgaagat atgagaacta acataccagg tgtatttgca gcaggagatt gtagatctaa
     3601 gactctaaga caggttgtaa ctgcaactaa tgatggagca attgcatcta tagtagctga
     3661 gaaatacatt gatgaaaaat ttggaaacta gcatctgtaa attaagaaat agtgtctgta
     3721 aattcaatga ataatattta aaattaaaat aaaaatttca agggggaata agaaaatgtt
     3781 agatttagat aaggctacat ttgaagaaga agttttaaat gcagaaggtt ttgtatttgt
     3841 tgatttttgg agtgaaggtt gtgagccttg taaagcttta atgccagatg ttcataaatt
     3901 agctgagact tatggagata aaataaaatt ctgtaagatg gatacaacaa aagctagaag
     3961 attagctata aaacaaaaag tattaggtct tccaactatg gctatataca aagatggaga
     4021 aaaagtagac gaagttacta aggatgatgc tacagttcct aatatagaaa acatgattaa
     4081 aaaatatctt taatatgttg ataaagtatt aacattaaaa gaaaaaatct atttttctct
     4141 gaagataacc tgaagataag aagaaaaaga tttttatgtc caactttgtt aaaaaaataa
     4201 ttttaaataa aataattttt ttacaataaa tattttttag taataaaata aagatttaag
     4261 acataaaaac acaataatat atctttaaaa aaataagaca aattgtaaaa tgatataatt
     4321 ttgagaaaaa ctcaaatgca tatacacaaa ttaaataaca ggaggtgcaa gtatgcgtct
     4381 tgaattaggg aaaatcttta taaaagatat tcaatttggt gatgtaacag aagtaaaaga
     4441 tggagtatta ttcatcaata agcaagagat gctacaagaa attggtggag atgagcatat
     4501 taaatctata gatatagaat tagctcgtcc aggtgaaagc gtaagaataa cacctgtaaa
     4561 agatgttatt gaaccaagag taaaagttga aggtaatggg ggaatcttcc ctggtatcat
     4621 gtctaaagtt gacactgtag gagaaggaaa aactcatgct ttaaaaggtg tagctgttgt
     4681 aactactggt aaaatagtag gtttccaaga aggtataata gacatgactg gagaaggagc
     4741 taagtatact ccattttcta aattaaataa tgtagtagta atagctgaac ctattgatgg
     4801 tttaaaacaa tacgctcatg aaaaagctgt tagaatgata ggattcaaag cagcaatgta
     4861 cttaggagaa gtagcaagaa atcttactcc agacgaagtt tcagtatatg aaactaaacc
     4921 tcttcttgaa tcaataaaag aatatccaga gcttccaaaa gtaggatatg tttacatgct
     4981 tcagactcaa ggtttattac atgatactta tgtatatgga gtagatgcaa aacaaatagt
     5041 accaacttta ttatatccaa cagaattaat ggacggagca attgtaagtg gtaattgtgt
     5101 ttctgcatgt gacaaaaacc caagttatgt tcatataaac aatggggttg ttgaagattt
     5161 atatgcaaga catggtaaag atataaactt tgtaggtgta attataacta atgaaaatgt
     5221 ttacctagca gataaagaaa gatcttcaaa ctggacttct aaattatgta aatatttagg
     5281 attagatgca gttatagttt cacaagaagg ttttggaaat cctgatactg accttataat
     5341 gaactgtaag aagatagaaa tgcaaggtgt taaaacagtt atagttacag atgagtatgc
     5401 tggacgtgat ggaggttctc aatcacttgc agatgcagat gtaagagctg atgctgttgt
     5461 aacaggtgga aatgccaacc aagttgtagt tcttcctaag ttagataaag ttattggaca
     5521 cttagaagta gtagatgtaa tagctggtgg ttctgatgga agtttaagag ctgatggaac
     5581 tattgaagtt gaaatccaag ctataacagg tgcaactaat gaaacaggat ttggtcattt
     5641 aactgctaaa ggatattaag aagtatttta ttaaacgatt gagtaatgtt aataaaacat
     5701 attatagata attataataa gtgttataga tatataaaaa tatattagaa atataataag
     5761 gaggattatt atgagtttac ttagtaataa aaaggttctt ataataggtg accgtgatgg
     5821 tataccagga cctgcgatag aagaatgtgt aaaaacagta gaaggagcag aggttgtttt
     5881 ctcatctaca gaatgctttg tctgaacagc tgctggggct atggacttag aaaatcaaaa
     5941 cagagttaaa gatgctgctg ataaattcgg agctgaaaat gttgtgattt tactaggtgc
     6001 tgctgaagcc gaagctgcag gtcttgcagc cgaaacagta actgctggag atccaacttt
     6061 cgctggacca cttgctggag ttgccttagg attaagtgtt taccacgttg ttgaggaacc
     6121 aataaaatca ttatttgatg aaagtgtata tgaagaccaa ataagtatga tggaaatggt
     6181 tttagaagtt gaagaaatag aagaagaaat gtctggtata agagaagaat tttgtaaatt
     6241 ttaatgccta aaagcaaaag gggtgaaact aagagatggg aaaactaaaa gcagtacatt
     6301 atattaatca gttctttgct ggtataggtg gagaagaaaa agctgatact aagccacatg
     6361 tggcagaaac tttacctcca ataagtttac aacttgataa attattaggt gaagatattg
     6421 aaatagttgg aacagtagtt tgtggagata gctatttcaa tgaaaatatt gatagtgcaa
     6481 gtgaagaagt tttatcaatg gtaaaaggtt ttgaaccaca acttttcata gctggtcctg
     6541 cgtttaatgc tggtagatat ggagttgctg ctggaacaat aactaaagtt gtaaaagatg
     6601 ctttaaatat accagcttta acaggtatgt atgttgaaaa tccaggagca gacatgttca
     6661 aaaaggatgt ttatgtagta gaaacttctg attctgcagc aggtatgaga aaagcacttc
     6721 ctaagatagc taaacttgca gtaaaacttg ctaatggaga agaaattgga actcctaaag
     6781 atgaaggata catagctaga ggaataagag ttaactactt ccatgaagac agaggttcta
     6841 agagagctgt tgacatgtta gttaagaaaa taaaaggtga accatttgaa actgaatacc
     6901 caatgccaaa ctttgataga gttgacccaa gtaaagctgt aaaagattta tcaaaatgta
     6961 aaatagcttt agttacttct ggtggtatag ttccaaaagg aaatccagat agaatagagt
     7021 cttcttcagc ttctaaatat ggaacttatt ctatagcagg agttatggat ttaacagaag
     7081 agacttatga aactgcacat ggtggatatg acccagtgta tgcaaattta gatgcagata
     7141 gagtattacc tgttgatgtt cttagagatt tagaaaaaga aggtgtaatt ggaaaattac
     7201 atgagacttt ctatacaact gtaggtaatg gtacatctgt tgcaaactct aaaaaatatg
     7261 cttctgaaat tggagcagca ttagtggctg atggtgtaga tgcagtaata ttaacttcta
     7321 catgaggtac ttgtactcgt tgcggtgcaa cgatggtaaa agaaatagaa aaaactggac
     7381 ttccagtagt tcatatgtgt acagtagtac ctatttcatt aacagtaggg gctaatagaa
     7441 tagttccaac tatagcaata cctcatccac ttggaaatcc agcgcttgac cctacagaag
     7501 aaaaagccct aagaagaggc cttgtagaaa aagcattaaa tgctttaact acagaagtag
     7561 atggtcaaac agtatttgaa aaataaaaat aacacacaca tatattatat agataaaaat
     7621 ataaacacac actaaaaata aaaaataact atacacaaaa aaaataatat agatttcatg
     7681 aatggataaa ttggagttat tcattcatga aatcttaaaa aatgccaaaa tacataaaca
     7741 ataatagaat caaatataca ataaaataca atgattagaa cttttaataa gtttaataag
     7801 attaaaaaat aagtgttcat aaaatttatg gaggtgcaca aaatgactta tccagtttta
     7861 aaaggggcag gctatgtact tattcatact ccagatatga tagtgcaaaa tggaagtact
     7921 tgtacagttg aaagagcaac aaatccagat tcagaatttt taaaagaagt aagtaatcat
     7981 ataagaagtt atgaagatgt agttaactac atgccaaatc aagtttatat aggaaataga
     8041 agaccagaag aattaagaga tttaccaatg ccatggtgtg aacaaaagat tgagggaact
     8101 agaaatggta agtttggaga aataatgcct caagatgagt tcatagcttt aatgcaaata
     8161 agtgatgcat ttgatttagt aaaattatca caagaattta ttgatgaagt aaagccaaaa
     8221 atcgaaaata actaccctga aattgcacca tttgtaggta aattaaaggg tgatgatata
     8281 gaagaaggta aagaattagt agcaacacac atagctgaag gattatacca tgatggaaaa
     8341 ttcgttggtt atgtaaaaag agcacatgat gtagatgtta acttaaatgc ccatacaatg
     8401 tttgaaaatc tagtagtaaa agcatctgga gttttatctg ctattcaaat gctaagacat
     8461 agcaaaatag accctgctga aatagattat gttattgagt gttcagaaga agcttgtgga
     8521 gatataaacc aaagaggtgg aggaaacttt gctaaatcaa tagctgagat agcaggactt
     8581 caaaatgcaa ctggttctga cacaagagga ttttgtgcag caccaactca tgcattaatt
     8641 caggcagcag ctttagtaaa agctggaata cataaaaatg taatggtagt agcaggtgga
     8701 gcaagtgcta aacttggaat gaatgctaaa gaccatgtta aaaaaggtct tcctgtactt
     8761 gaagatgttg ttggaggatt tgcagtactt gtatctgaaa atgatggtgt aaatccagtt
     8821 ataagaactg atttaacagg taagcatact gttggaactg gttcttcacc acaagcagtt
     8881 atgacagcac ttataacttc aggtcttgat agagctaatt taaaaataac agatgttgat
     8941 gtgtattcag ttgaaatgca aaacccagat ataactaagc cagcaggagc aggagatgtt
     9001 ccagaagcaa actacaaaat gataggagca cttgctgtta aacgtggaga cttagaaaag
     9061 aaagaattga aagattttgt ttctaacaaa ggattaccag gatgggcacc aactcaagga
     9121 catataccat caggagctcc ttatatagga tttttaatag atgatttaac tacaggaaat
     9181 agaaatagag ctatgatagt tggaaaaggt agtttattct taggaagaat gactaactta
     9241 tttgatggag tatcttttat agctgaaaga aatacaggtg ttactgaaga gacttcagga
     9301 atatctaagg atgaaattaa gaagataata gctgaatcta tgaaaaaact agcattagac
     9361 atgttagaag aataggggtg aggatatgtc aaaaaaagta atcgcagatg tattcttaga
     9421 agtagctaat gcaatagaaa gcggcgaatt tggcaaaaaa gtaaaaattg gtgttactac
     9481 acttggaagt gaacatggtg tagagaacat ggttaatggt gcacaattag caaaatctaa
     9541 tttatttgat attgtgctta ttggaccaaa agtggaaact gatcttgaag tagttgaagt
     9601 taatgatgaa aaagaaatgc atgcaaaaat ggaagaatta ttagattcag gatatattga
     9661 tgcttgtgtt actatgcact ataattttcc aataggtgta tctacagtag gtagagtaat
     9721 aactccagca aagggaaaag aaatgatact tgcaacaaca acaggaacaa gcgctactaa
     9781 tagaatagag gcaatggtga gaaatgccat ttatggtatt gctacagcta aatcaatggg
     9841 taacaaatgc ccaaaagttg gaatacttaa tgttgatgga gcaagacaag ttgagaagtg
     9901 cttaaaagaa ttaaaagata atggatatga catggaattt gcggattcaa ttagagcaga
     9961 tggtggatgt gtaatgagag gtaatgattt acttgtaggc gcacctgatg ttatggttac
    10021 tgatacatta tctggaaata tatttatgaa agtattttct tcatatacta caggtggaga
    10081 ttatgaagct caaggctttg gatacggacc tggagttgga gaggactatg atagaaaggt
    10141 acttatagta tcaagagcat caggttctcc ggtagtagca aatgctttaa aatacgctta
    10201 tgatgttgta aagggagata taagtaatgt tgcaagaaat gaatttgcaa aggttaaaaa
    10261 agcaaaattt gatgacatta tatcttctct tactaaaaaa gaagttaaag ctgaaaaagt
    10321 agaagttaaa atgccagata aagagattgt tactcgtcaa atagctggtg ttgatataat
    10381 ggatttagaa gatgcagtat ctgagttatg gaaaaacgga atttatgctg aaagtggtat
    10441 gggttgtaca ggtccaatag ttttagtaaa tgaagctaaa ggagatttag cagttgaaac
    10501 tttagttaaa gctggatata ctgcaaaata agtacatttt ttatatgtaa ataagaacat
    10561 atagttataa tcttattgat tataattata tgtttttttt attttatatc ttgtcaatat
    10621 ttatatatta acacaatcca tttatttgat tctctagcta atttattata tttgttcaat
    10681 taaaattaag ttttctaact aaataaaact aatgaaatat taaagtgaaa ttaataaaat
    10741 ataaaaaaat tttagaaata aactatctgt tagataaaaa gaagattaat tagtgatata
    10801 atataattaa gataaaattt attatttgga ggtaaaatca tgagcagagt aaaaaatgta
    10861 gtagaattgt tggaaactaa aggtgttgat gcactatatc taacaaagaa aactaatgta
    10921 aactatataa gtggttttcc tgatgaagaa gcatatgcag tgatttgcaa agatggaaac
    10981 tttttagtta ctgatagtag atatatggag cttgcagaaa aggtttgcaa agattttgaa
    11041 attataaatt ggcataattt tgatagaagt gtggcaaagg cagttaaaag tgtatgtgat
    11101 aaagtgggta taaagaaact tgggtttgaa agaactaata tagtgtttga taaatatgaa
    11161 gaacttaaaa atctgattga gaaagataat ggagaattaa tacctacaga aaatattgta
    11221 gagactttga gatatgtaaa agacaaagat gaaatcaaaa atacaagaaa agcttgtgaa
    11281 atagcggata aagcattaga agaattgatt ccacatataa aagctggagt tagcgaaatt
    11341 gaactggcta caaagttaga gtactttatg aagatgaacg gagcacaaaa tataggattt
    11401 gaaactatac tgatatcagg agcaaagact tcattgcttc atggtaagcc tagtgataag
    11461 attatagaaa aaggtgattt tgtacttata gactatggtg ctatgtataa tggttatata
    11521 tctgatacaa ctagaacttt tatagttgga gaagcttctg aaaagcaatt agaaatatat
    11581 aatttagtta aggaagctca aaatgtaggg gttgagaaca tgaaagttgg tgtacatgcg
    11641 acaattccag atgctgagat aagaaaagtt gtcaaaaaat atgaagacta ttactatcaa
    11701 ggtataggac atggagtcgg tagagatgtt catgaagaac ctttcattgg caattatggt
    11761 gataaaatca tagaagaagg atgtataata acaatggaac caggtattta tttccctgga
    11821 tggggtggag ttagaataga agatactgtt ttgattacta aaaatggtcc agaaagactt
    11881 actaaattcc ctaaagattt gatgatttta gacaaataat ttaatttata tattttttta
    11941 cataaaatat aacataatct cactattaag ccctatttac atattatagt aaagagggct
    12001 taattttatt gtgagaggtg atagtatata tgtataaaaa acataaattt ataaaaaata
    12061 taaaaaaagc ctggtacaaa atcaaactaa ttgatagatg tcttataatt gtaatggcaa
    12121 tactaatgtt tcaatctata tataatttat ttgttaatga agtaaattca caagatacta
    12181 caactattga tgtagtagta agaaccactt cagcagcgat atttggatat tttttaagtg
    12241 ctaattttat aaagagacct tcaaggaagt atacaaatac tgatgtatct tcaaatcttt
    12301 ttatagataa taatttatcc ccaaaaggtc aagagtcaag tcaaaataat gtaatgaatg
    12361 tcaaaaatac cattggattt acttcagaag ctgataatta tgaaaagaaa atacctatta
    12421 ataataatga aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt atagccactg
    12481 taatttgtgt agtagcatta acagtacttt ttattgtaag aaattttaca actacaacat
    12541 cagcttcttt aggtactatt tcacagatga gagattttgt ctcaggatgt gtgggttttt
    12601 tgctaggttg tccaagtaga cctgattaaa aaagtattat gaataaaagg tattttaaaa
    12661 agtatcagta taataatatt tagatttaaa tgtatacaat tataattcta taacgttcta
    12721 tattgcattt ttatactgat actctaaact atactattta ttaaaatatt ttaacattgt
    12781 atttaaacaa aagattatat ctgtacaata taaatttagt ttatatggat agatgccata
    12841 tactcaataa aaagtttcat actatgagat atgtattgat ttcttctcca aagaactcca
    12901 atatttgcag taattggacg ttctaatggt attgaacaga gattttctgt attttgaaat
    12961 atttctttat acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa
    13021 agttggtttg aatgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc
    13081 ttatagtgat aagaaccttc agataagatt acaaaaggta cattttttaa catataattc
    13141 gatataatgg gatgttttgc caattcattt ttgggatttg taactaagta caattctgtt
    13201 ttatagattg tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa
    13261 aaatcaagtg tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt
    13321 ttaagctcta tatctggata aattacatta aatctaggaa gtatttttga aagcaaaaac
    13381 gtacctaaaa ctgtaggaat acctattttg agagaagctt ttctcttttt tcctaaatca
    13441 agtgcgtcac attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa
    13501 atttttcctt caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca
    13561 agttcttttt ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt
    13621 tccgcagcct tagtcatgtt tttatattta cagacagttt gaaaatagta taaatagttt
    13681 atattcattt ggtattagtt cctttcattt acgtattagt gacaagtata taactaacaa
    13741 tagtataagt tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata
    13801 agtttttgat attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat
    13861 agctggtgta gcttagagta aatcaatcta tattacagtt atatcaacga atattaaaaa
    13921 attatctatt aaaaaataaa aattattttt acaattaaat tgttaattaa atcgtaaaga
    13981 aaaaaatgat attaacaaga taaaattttt ttaatcagaa atgttaattt aataacaaaa
    14041 tagagtcttg gggagaaagg agaaatatat tttttaataa tatttgttaa tttaataacg
    14101 aaaaatgaaa gggagtgatt tagtgaagtt tgaattaatg gattttttaa tgaatccttt
    14161 tgtccttatg tttgctgcag taattacagg aattttattt ggaaaaatta aatttgggaa
    14221 gtttaatttt ggggtatcag gagcattatt tacaggatta tttattggat ggcttgcata
    14281 tagcttgggg aatctaataa tcgcaaaagg ggaaacagca gcaggatata aggcagcaac
    14341 tgtaatgatg ggaaatggaa ttatttcttc tgacttcttt gatttctttt taataatatt
    14401 tgttgcagca gttggtctat tggcagcaaa agatatgaaa gctgtattaa agaagtatgg
    14461 agcaagattt gtaattttag gagttcttat aacttttata ggtggctcta tgacttatgc
    14521 aatgacttta ctatcaagtg ataaaggaag tagtgcttat gaagtttctg gtgtttacac
    14581 aggagcatta acaagttcgc ctggattagc tgctgcttta gaaacagctg gtaaacatgc
    14641 agaagatgta tcaaaagaat ttgaaaaagc ttcaataaaa gataaaaaag aaattttaaa
    14701 agtagttgac ccagaaggca aattggatgt gaatacaact acatcactga ctcaagagca
    14761 aatagataag tacattgcat atgctgaagc tggtgttggt ataggtcatg cagtcgcata
    14821 tccttttgga gttttaatag taatcttagg tgtaaatttc cttccaaaat tatttaggat
    14881 ggaccttaaa gaagaaagaa gaaaatatga gaaggaaatg aaagaagcaa gagatagtgt
    14941 ttctggtaaa acgataccag aagttccatt taatataata acatttttct tgacatgttt
    15001 agctggatac ttagttggag gaatacatgt atttatggga ccactagggt acttcacact
    15061 aggagcaaca ggaggttcac taattgtatc tctagttctt ggatatatag gtaagatagg
    15121 ggtagtaaat ttcagaatgg aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt
    15181 attcttctta gcaatagttg gattaagata tggtggaaaa gtagtagatt caattatgac
    15241 atctggtatg cacctagctt tagtagcaat agcagtaggt gttacagcta tgatgatagg
    15301 attcttagtt ggtaaatatg tatttaaatt aaattggatt ttactatctg gagctgtctg
    15361 tggtggtatg acatcaacac caggtcttgg agctgctgtt gatgctcttg atagtgatga
    15421 cccagcagca ggttatggtg ctacgtatcc atttgcatta ttaactaaag ttatattggt
    15481 tatagtttta cacaaacttc caatgtaaat aagattttat atggggggat atttatgaat
    15541 aatgaaggag catttgaaga attaagtgga agattaagaa ataaagaatt gtgtgattta
    15601 ataatgaatg cagatgaagc tgctaaaatt atagaggatg gcatggttgt tggagttagt
    15661 ggatttacac cttcaggcta tccaaaagca gtaccattag cagtttcaga aagagctaaa
    15721 agtggagaaa agataaaatt aaccgtatat tcaggagcat ctttaggtcc agaagttgat
    15781 ggagcatggt cagaagcagg tataattgaa agaagacttc cttatcagac taattcaaca
    15841 cttagaaata atattaataa aggtgttgtt gattatatag atatgcacct aagtcattct
    15901 actcaatttt taaattatgg aactatacct aaagtagatg tagctatagt agaagcactt
    15961 gcaataactg aagatgggaa tattatacca actagtggga taggtaattc tccatctttt
    16021 ataaaaagtg cagataaagt aatagtagag ataaatcttg caaaaccaat ggaaatggaa
    16081 ggtatggcag atatatatat tacggaaaat ccaccaaata gaaaaccaat agaaataaat
    16141 catcctaaag atagaattgg aacaacatat ataccttgcg gtttggataa aattgcagga
    16201 atagtaatta ccaatatgca agataagacc cgtccattgg gtgttgtaga tgaagcttcg
    16261 aaaaaaatat caaataatat tattactttt ttaagagaag aagtaaaggc aggcagatta
    16321 tcaaagaatt tacttcctct tcaatctggt gtaggaagtg tagcaaatgc agtattatat
    16381 ggattatgtg aatctgaatt tgaaaattta acttgttata cagaagttgt acaggattct
    16441 atgttggatt taataagaat gggtaaagtt acaatggcat ctactacatc tgtaagtcca
    16501 tctccagaag gattaataaa atttgaagaa gatattgatt tttttaagga taagattata
    16561 ttaagacctc aagaaattag taataatcca gaaatagcca gaagaattgg tgtaattgca
    16621 atgaacactg caattgaagt tgatatttat ggaaatgtaa attctactca cataatgggg
    16681 tcaaagatga tgaatggaat tggtggttct ggtgattttg ctagaaatgg agcaataact
    16741 atatttagta ctgagtcaat tgcaaaaaat ggtgatatat catcaatagt acctatggta
    16801 tctcacgtag accacactga acatgatgta atggtaattg taacagaaca aggatatgct
    16861 gatttaagag gtcttgcacc tagagaaaga gctataaaaa taatagaaaa ttgtgcccat
    16921 ccagattata aagaacaact tagggattat ttaaatagag cttgtaaaag tggagcaaaa
    16981 caaacacctc acatattgga cgaagcttta tcatggcata gtaaatttat gagtacaggt
    17041 acaatgaaaa aggctgaaac ttttaaaagt gcattataaa atatttaata aattggaggt
    17101 agattttaaa tggaaaaagc agtggaaaac tttgaagatt taagtaagga atacattaat
    17161 ggttatattg agagagcaag aaaagctcag agagagtttg aatgttatac tcaagaacaa
    17221 gtagataaaa tagtaaaaat agttggaaaa gtagtgtatt ataatgctga atatttagct
    17281 aaacttgctg tagaagagac tggaatggga gtatatgaag ataaggtagc taaaaataaa
    17341 agcaaagcaa aagttatata taacaattta aaagataaaa aatcagttgg tataattgat
    17401 atagatagag aaactgggat aacaaaggta gctaaaccag ttggagtagt agcggcaata
    17461 actccttgta caaatccaat tgttacacct atgagcaatg caatgtttgc acttaaggga
    17521 agaaatgcaa ttattatcac acctcatcat aaagcaattg gatgtagtac aaaaactgta
    17581 gagatgatta atgaagaatt agaaaaaata ggagctccag aaaatttaat acaaatactt
    17641 gaccaacaat caagagaaaa tacaagaaat ttaatttcat cagcagatgt agtaattgcc
    17701 actggaggaa tgggaatggt aaaagctgcg tactcaagtg gaaaaccagc acttggtgta
    17761 ggggcaggaa atgttcaatg tatcatagat agagatgtag acattaaaga agcagtacct
    17821 aaaatcatag caggaagaat ttttgataat ggtatcattt gttcaggtga acagtcagta
    17881 atagtagcag aagaaatgtt tgataaaata atggatgaat ttaaaaataa caaaggtttt
    17941 atagttagag ataaagcaca aaaagaagct ttcagaaatg ctatgtttgt aaataaatct
    18001 atgaataaag atgcagtagg tcaatcagtt catacaattg caaaaattgc aggtgttgag
    18061 ataccagaag atacaaaaat aatagtaata gaagctgatg gaccaggaga agaggatatc
    18121 attgcaaaag aaaaaatgtg tccagttata tctgcataca agtataaaag ttttgaagaa
    18181 ggtgtagcta ttgcaaaagc aaatttaaat gtagaaggaa aaggacatag tgtatctata
    18241 cattcaaata cagttaaaaa tatagaatat gcaggagaaa atatagaagt atcaagattt
    18301 gttattaatc aatgttgtgc tactagtgct ggaggaagtt tctttaatgg tcttgcacct
    18361 actaatactt taggttgtgg ttcttgggga aataacagta tctctgaaaa tcttgattat
    18421 aagcatctaa ttaatatttc tagaattgct tattatatgc cagaaaatga agtccctaca
    18481 gatgaagaac tttggggata aaatttagac aaaaaaaata ttcattctaa attaaagtag
    18541 gaatatattt taatttagaa tgaatttaag acataaatta aatttaaatt agtttattga
    18601 actaaattta aaatattaag agacatatag aagaaggaag tattataatt taaaataaat
    18661 aaaaaaatct ataaaagaga aggagtggtt ttattatggc attaatgaca ggagctcaat
    18721 atattgaaag tttgagaaaa ttaaatacta aggtttatat gtttggtgaa gaggttaaga
    18781 attgggttga ccatcctatg attagaccat ctataaattg tgtagcagca acttatgact
    18841 tagctcatga cccagaatat gcagatttaa tgacagttac ttcaaatatc acaggtgaaa
    18901 aaataaatag atttggtcat cttcatcaaa gtgttgatga tttgataaaa aaagtaaaaa
    18961 tgcaaagact atgtggtcaa aaaacagctt cttgtttcca aagatgtgtt ggtatggatg
    19021 catttaatgc agtgtattca acaacatttg agtgtgataa agcccatgga actaattatc
    19081 atgataattt tgtaaaatac ttaacttata tacaagaaaa cgatttagta gttgatggag
    19141 ctatgactga ccctaaagga gatagaagtt tatcacctag tgctcaacct gacccagata
    19201 tgttccttca catagtagaa agaagagaag atgggataat tgtaagagga gcaaaggcac
    19261 atcaaactgg ttcaataaat tcacatgagc accttataat gcctactata agtatgactg
    19321 aagcagataa agactacgct gtatcatttg cagttccttc agatgctgaa ggagtgttta
    19381 tgatttatgg aagacaatct tgtgacacta gaaaattaga agaaggtgca gatgttgatt
    19441 taggaaataa ggagtttgga ggtcaagaag ctctagtagt atttgataat gtatttgttc
    19501 caaatgatag aattttctta tgtggagaat gggatttctc tggtatgtta gtagagagat
    19561 ttgcaggata tcatagacaa agttatggtg gatgtaaagt aggagtaggt gatgtaataa
    19621 taggagcagc agctttagca gctgattata atggagcaaa caaagcttca catataaaag
    19681 ataaactaat agaaatgact caccttaatg aaagcttata ctgctgtggt atagcatgtt
    19741 catcagaagg tcacaaaaca gaagctggaa actatcaaat agatttactt cttgcaaatg
    19801 tgtgtaaaca aaatgtaact agatttcctt atgaaatagt aagacttgca gaagatatag
    19861 ctggagggct tatggttact atgccttcag aaaaagactt taaatcagac cttaaagttg
    19921 gaacaagtgg aatgactatt ggcgaagtgt gtaacaagta ttttaaagct tcttcagtgg
    19981 cttcaacaga agaaagaatg agaatattaa gattcttaga aaatatatgt cttggttcat
    20041 ctgctgttgg ttatagaact gagtcaatgc atggggcagg ttcaccacaa gctcaaagaa
    20101 taatgatttc aagacaagga aacataaatc aaaagaaaga attagctaag aaaattgcag
    20161 gaataaaaaa agaggaagct ttaaatttat aaaaagttga tatataaagg gaaattatat
    20221 attaatgtgt attaattaat gaaaaaatat gtagtataag agataattat aaaattatgg
    20281 tggtttttag ctaagtaaag aaatttttta taaggagaat ttacacatga cctgtggagt
    20341 tagattttgt gggggctgta accctagata cgaaagaggg aaggctctta caataatcaa
    20401 agagcacttc aatggcaggg tagattttag tatagcacaa gagaacgtcg agtatgattt
    20461 gctacttata attggaggat gcgcaactct atgcgcatcc tatcaacaat ataaagtgaa
    20521 aaatgaatgt gttaagatgt gggattttag ccatatagaa agagttataa atgtgattga
    20581 atcagcaatt tgtgaaactt aaaaaagcta aatgggggga tttcattgag ttggcgagaa
    20641 ttgtatcaaa gtaaattatg tagtgctaca gaagctgtaa aacagattaa aaacggagat
    20701 actgtagtat ttgcacattg tgtaggtgaa cctcctgcac tagtagaggc tatgattgaa
    20761 aatgctgaac aatataaaga tgttgagatt aaacatatgg taagcttggg tagtggtgga
    20821 tatactgcta aaggaatgga agctcatttt agagtaaatc caatgtttgt aagtggaaat
    20881 gtaagaaagg caattgaaaa tggagatggt gattttacac ctacattctt ccatgaagta
    20941 ccaaagttgc ttagagaaaa aagattaaaa tgtgatgttg ttttagcaca ggtaactcca
    21001 ccagatgaac atgggtattg tagcttagga acatcagttg attatactta tgaagccatt
    21061 aaaactgcta gaacagtaat tgttcaagtg aatgaccagt ttcctagaac ctatggtgag
    21121 gtagtccatg tatcagagtt tgactatata gtggaaaaat cacaaccttt atttgaacta
    21181 caacccgcaa agattggaga agtagaagaa gcaattggta aaaattgtgc ttcattaatt
    21241 gaagatggtt ctactttaca gctagggatt ggtgggatac cagatgcagt tatgttattt
    21301 ttaactgata aaaaggattt agggattcat tcagaaatga tttcagatgg gactttggcc
    21361 ctttatgaaa aaggtgttat aaatggtaaa tataaaaatt ttgataaaga aaaaatgaca
    21421 gttaccttct taatgggtac taaaaaatta tatgactttg caaataataa ccctgcagta
    21481 gaggtaaaac cagtagacta tgtaaatcat ccagcaatta taatgaaaca acataagatg
    21541 gtttctataa attctgctat acaagttgat ttaatgggac aggtagttgc agaggctatg
    21601 ggacttagac aatttagtgg tgttggaggt caagttgact ttatacgtgg agtgtctatg
    21661 ggtgaagatg gaaaggcaat tatagctatg ccttcaataa ctacaaaaaa agatggtaca
    21721 gtaatttcaa aaattgtatc tattgtggat gaaggtgcac ccataacaac atcaagaaat
    21781 gatgttgact atattgtaac agaatacggt atagcagaat taaaaggaaa atctcttaga
    21841 gaaagagcaa gaaatttaat aaatatagct catccaagtg taagagaaag tctagcagta
    21901 gaatttgaaa agagatttaa ggagaaatat taataataga ggggtgttaa taatggaagc
    21961 acttagagta gttccaaaga tattttattt tgatacattt aaagagttta atgaagaatt
    22021 taaaatagga aaaaatgatt tagtaattac taatgagttt atatatgagc catatatgaa
    22081 acctcttgga atagatacaa atttaatttt tcaagaaaag tttggaacag gtgaaccatc
    22141 tgatgaaatg atagactcta tgacaaagga aatgaagaaa tacaatttta atagaattat
    22201 tgcgtttgga ggaggaacaa tagttgatat atgtaagata ttagcacttg atgttccaga
    22261 aaagtctatt gatttatttg aaggagaagt atcacctaaa aaaataaaag agttagtagt
    22321 agttcctaca acatgtggta caggtagtga agtaacaaat gtagctattg cagagttaaa
    22381 atctaaacat actaaaaagg ggcttgcagt agaagaaaca tatgcagact atgcagtgtt
    22441 aattccagag acagtaaaag ggttacctta caaattcttt gttacaagtt cggtggatgc
    22501 tcttatacat gctattgagt cttatctttc tccaaaagca agtccattta cagagatgta
    22561 ttctctacaa gcaataaaaa tgattatgga tgggtataag aagatagttg ataaaggaga
    22621 agaagagaga tttaatcatc ttagagactt tgtacttgca tcaaattatg caggaattgc
    22681 ttttggaaat gctggttgtg cagcagttca tgcactatct tattcaatag gtggagcttt
    22741 ccatgttgca catggtgaag caaattatca gttctttact gaagtattta agatgtattc
    22801 tagaaaaaaa cctaatggaa aaattataaa atgtacaaaa attttagcag atgcccttga
    22861 atgtgaccct aattgtgatg tttatggaga gctagaaaag tttttaaata aattaatagc
    22921 taaaaaagct ttaagagagt atggaatggt agaaagtcag atagatgaat ttacagatag
    22981 tacaatagcc aatcaacaaa gattacttgc taataattat gtagagctta gtagagaaga
    23041 aataagagag atatttgcaa atctttatta atacaatttt ttatttatat gtatagtcaa
    23101 cataaacaat gctaattata tttgtgtaca aataaaaatg agggaactta aatttaatta
    23161 aagttaagtt ctcttatttt tatatggagt taaatataag tatattttta atttaaaata
    23221 tgcttatatt taagtctatt actttatttg aaacaatttg tagaatttca aattccttga
    23281 cagtaataag taagtaattg tataatcaaa ctgcaatata ttaataatat tagtgaaaat
    23341 gaaaggatat aaaatatgta taaatcagaa aatttttcaa aaaaatctgt attaataaat
    23401 tcatttttaa gtaaattttt aaaaataatt ggatggggtt tggtaagctt atttacatta
    23461 tcagccttag tactttcaac tatttccatt ataattggat gggctgtaat acttcctatt
    23521 caagtatgtg cagtattttt acttatagga ggagtttgta tttttaaagg taagagatta
    23581 gcagaccaaa tttctcgtta taaaaaatat tgtacaatta taaacaatag aaatattatt
    23641 ccagtagaac ttattgcaga aaacacatct aaatcattaa attttataat aaaagatgtg
    23701 caaaagatga tagacaaaaa ctactttata aatacttata ttgataaaag aaataatcag
    23761 attgtgttaa caaatgaaga ttttattcca ccaagatatg aagatgtagc atatgaagtc
    23821 aaagaagaag aaaagactta tgaatccaat gaagttgata caattataaa taaaggaatg
    23881 aattatctaa cacaaataaa agaagctaat aaaaatatta aatcagaatc aatgggcgat
    23941 aaaattgtac aagtagaaga tgttacaagt aaaatatttg atgttgtaaa acatgaccca
    24001 tcaaaactta ctcaaataca aaaatttatg gattattatc ttccaaccac tttaaaacta
    24061 ttaaattcat atcatacatt ggaagaacaa ggaattgata gagaaaatat aacaacaact
    24121 atggaaagta ttgaaaatac aatgaatacg attgtagtag cttttgaaaa tcaattggac
    24181 tatctgtttg aagatgaagc cattgatatt tctacagata ttactgtttt agagaatatg
    24241 ttagtccaag aaggattaac gagtggtaaa ttttaacttc aatagaaaaa atattctgtt
    24301 atttttaaac aattaattag tcataaagta taaaatatgt attatataag ttaatcagta
    24361 ctttatgaag gaagaggaga gaaagataca atgaataatt tagatgatat accagttatg
    24421 ccaactttga cacttgaccc atttggagaa gaatcaaaca ctaatgatat agataattct
    24481 gacttattaa tgaaaaaaga tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa
    24541 cgcaagatgg taaaggaatt tgcggaaaaa atagatatta caaatacaaa tatgattttg
    24601 caatatggag ctggtgctca aaaaaaggta gcaggctttt ctgaaacagc tttaaagagt
    24661 gtaaaaacaa gagatttagg agaagttgga gatatgctta caaatctagt tactgattta
    24721 aaagcatttt cagcagatga aaaagaacaa agtggttttt tgggaatttt taaaaaggca
    24781 aataataaaa tttcaaatct aaaaactaag tatgatagtg cagaggtaaa tgtggataaa
    24841 gtaagtaaag agcttcaaaa gcatcaggtt aaaatattga aagatatagc aatgcttgat
    24901 aagatgtatg aattgaactt agcttactca aaagagctta ctatgtatat tatagcagga
    24961 aaacaaaaat tgaaggatat gaaagaatat gagatgccaa agttgagaga aaaagctcgt
    25021 ttatctggtt caacagaaga tgctcaatct gtaaatgata tggtttcact ttgtgacaga
    25081 tttgaaaaga agattcatga cttagaatta actagaatgg tatcgcttca aatggctcct
    25141 cagataagat tggttcaaaa tagtaataat ctgatggcag aaaagattca atcaactata
    25201 gtaaatacca ttccattatg gaaaaatcaa atagtgcttg ctcttggaat ttctcactta
    25261 aatcaagcta tgaaagcaca gcgtgaagtt agtaatatga caaatgagct tttattaaag
    25321 aatgcagaaa ctttgaagat gggaacaata gaaacagcaa aagaatcaga aagaggaatc
    25381 gtagatatag acacaattaa gaaaactaat caatctctaa tatctactat tgatgaggta
    25441 gttaagatac aacatgatgg tagaataaag agacaagaag cagaagttga attatcaaaa
    25501 atagaaaatg aacttaaaag caagcttctt gaatttacag ttaaataaaa attatactaa
    25561 gataaaaaga gactgtctca aaatagagat aagtctcttt ttcattgttt aaatgattaa
    25621 agtatattta tattataaat ttctattatc ttttaacata acatcatgag ctccaccttc
    25681 aactatacta gtagaagata caagtgtaag tttagcgttt ttttgaagtt caggaatgct
    25741 aagtgctcca cagttacaca tagtagaacg aactttactt aaagaaagag ttacattgtc
    25801 tttcaaagaa ccagcataag gaacataaga gtcaacacct tcttcaaaag aaagtttttt
    25861 atctccacca aggtcatatc tttgccagtt tctagcacga gcagaacctt ctccccaata
    25921 ctctttcata taactaccat ttatattaac tttgttagtt ggactctcat caaaacgaga
    25981 gaagtatcta ccaagcatta taaaatctgc acccatagct aaagctaaag ttatatgatg
    26041 gtcataaacg ataccaccat cagagcaaat tggaatataa atacctgttt tttcaaagta
    26101 ttcgtcccta gcttgagcaa cttcaattat agaagtagct tgtccacgtc ctataccttt
    26161 ttgttcacga gttatacata tagaaccacc accaatacca actttaacga agtcagcacc
    26221 agcttcagct aggtaaagaa atccctcgcg gtcaactaca tttccagctc ctaccttaac
    26281 tgtatcacca tatttttcac gaatgaaatc aagtgttatt ttttgccact cagaaaaacc
    26341 ttctgaagaa tctatacata aaacatcagc accagcttca actaaagcag gaacacgttc
    26401 agcaaagtct ctggtattaa tacccgctcc aactacatat ctcttagaag aatctaaaag
    26461 ttcaagtgga ttttctttat gagaagagta atctttacgg aataccatat acataagatt
    26521 accattatca tcaagtacag gaagagaatt aagtttgtgg tcccatataa tgttgtttgc
    26581 ttcctttaaa gtaacatctt tgttagcata aactatagat gacataggag tcataaattc
    26641 actaacttta gtatctaaat ccatacgact tatacgataa tcacgacttg caacaatacc
    26701 taataattta ccagttgaag ttccatcttc agtaacagct actgtagaat gtccagtttt
    26761 ttcttttaag tcaagtatgt cttttaatgt gttatcaggt ttaatatttg aatcacttac
    26821 aacaaaacca gctttgtggg atttaacacg tgctaccata gcagcttcat cttcaatagt
    26881 ttgagaaccg tatataaatg aaataccacc ttctttagca agtgcaatag ccattttgtc
    26941 atcagaaaca gattgcataa tagcagaagt caaaggtata ttcatataaa tatctgcttc
    27001 ctcgcccttt ttaaatttag taacaggtgt ttttaaactt acattagcag gtatacaatc
    27061 agtagaagag aaacctggta caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa
    27121 ataaaacgcc attttttaat cactccttat atttttatac tttttataat tgatagtatt
    27181 aattataact tattctttaa ttttattaaa gtattttgtc aaatattgag atttttgaaa
    27241 aatttaaact attttaattg aatttgttta atatatattg ccttataggg taaagttgta
    27301 tattaaataa tgtttagtgt ttaatagtag ctaatttgaa attaatc
//
LOCUS       DAEMAE010000030        27308 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEMAE010000030 DAEMAE010000000
VERSION     DAEMAE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.030"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(81..263)
                     /locus_tag="KPH93_001031"
     CDS             complement(81..263)
                     /locus_tag="KPH93_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687865.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            576..995
                     /locus_tag="KPH93_001032"
     CDS             576..995
                     /locus_tag="KPH93_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG1687866.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSRDGEYVISNISIDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1126..2967
                     /locus_tag="KPH93_001033"
     CDS             1126..2967
                     /locus_tag="KPH93_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1687867.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLSKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENDFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLNDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNMTKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQGLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYIDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKDITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            2994..4880
                     /locus_tag="KPH93_001034"
     CDS             2994..4880
                     /locus_tag="KPH93_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1687868.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALIE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHNNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEAILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESMDVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEMLIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            4888..6888
                     /locus_tag="KPH93_001035"
     CDS             4888..6888
                     /locus_tag="KPH93_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1687869.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKVPPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            7150..8409
                     /locus_tag="KPH93_001036"
     CDS             7150..8409
                     /locus_tag="KPH93_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1687870.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDIAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            8470..10257
                     /gene="iorA"
                     /locus_tag="KPH93_001037"
     CDS             8470..10257
                     /gene="iorA"
                     /locus_tag="KPH93_001037"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG1687871.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            10259..10834
                     /locus_tag="KPH93_001038"
     CDS             10259..10834
                     /locus_tag="KPH93_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG1687872.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            10887..11957
                     /gene="buk"
                     /locus_tag="KPH93_001039"
     CDS             10887..11957
                     /gene="buk"
                     /locus_tag="KPH93_001039"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG1687873.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLKILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            12175..12876
                     /locus_tag="KPH93_001040"
     CDS             12175..12876
                     /locus_tag="KPH93_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687874.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHANN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKNE
                     EHKCKSSIVIKNEQN"
     gene            12936..13442
                     /locus_tag="KPH93_001041"
     CDS             12936..13442
                     /locus_tag="KPH93_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG1687875.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEESLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLSE
                     IDNNKLNI"
     gene            13617..14639
                     /locus_tag="KPH93_001042"
     CDS             13617..14639
                     /locus_tag="KPH93_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1687876.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(14786..15094)
                     /locus_tag="KPH93_001043"
     CDS             complement(14786..15094)
                     /locus_tag="KPH93_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG1687877.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(15154..15582)
                     /locus_tag="KPH93_001044"
     CDS             complement(15154..15582)
                     /locus_tag="KPH93_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687878.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(15805..17262)
                     /locus_tag="KPH93_001045"
     CDS             complement(15805..17262)
                     /locus_tag="KPH93_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG1687879.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKIYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            17680..18594
                     /gene="nadA"
                     /locus_tag="KPH93_001046"
     CDS             17680..18594
                     /gene="nadA"
                     /locus_tag="KPH93_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG1687880.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKSFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            18607..19908
                     /locus_tag="KPH93_001047"
     CDS             18607..19908
                     /locus_tag="KPH93_001047"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG1687881.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            19889..20725
                     /gene="nadC"
                     /locus_tag="KPH93_001048"
     CDS             19889..20725
                     /gene="nadC"
                     /locus_tag="KPH93_001048"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG1687882.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDESSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHMGAAGGVKNAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEEIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            20982..21314
                     /locus_tag="KPH93_001049"
     CDS             20982..21314
                     /locus_tag="KPH93_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG1687883.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            21476..21661
                     /locus_tag="KPH93_001050"
     CDS             21476..21661
                     /locus_tag="KPH93_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687884.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            21688..22773
                     /locus_tag="KPH93_001051"
     CDS             21688..22773
                     /locus_tag="KPH93_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG1687885.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     ISLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            22766..23224
                     /locus_tag="KPH93_001052"
     CDS             22766..23224
                     /locus_tag="KPH93_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687886.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKVPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            23259..24350
                     /locus_tag="KPH93_001053"
     CDS             23259..24350
                     /locus_tag="KPH93_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1687887.1"
                     /translation="MKLSKKIQALILFGLMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKVN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            24355..24579
                     /locus_tag="KPH93_001054"
     CDS             24355..24579
                     /locus_tag="KPH93_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687888.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            24589..25152
                     /locus_tag="KPH93_001055"
     CDS             24589..25152
                     /locus_tag="KPH93_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687889.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     ISDNNPKKMGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            25113..25742
                     /locus_tag="KPH93_001056"
     CDS             25113..25742
                     /locus_tag="KPH93_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687890.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            25782..25919
                     /locus_tag="KPH93_001057"
     CDS             25782..25919
                     /locus_tag="KPH93_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1687891.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            25919..26671
                     /locus_tag="KPH93_001058"
     CDS             25919..26671
                     /locus_tag="KPH93_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687892.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            26684..27169
                     /locus_tag="KPH93_001059"
     CDS             26684..27169
                     /locus_tag="KPH93_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687893.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIESTLQYFIDKNIMVVDGDSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
BASE COUNT        10799 a         2823 c         4864 g         8822 t
ORIGIN      
        1 agcttttaag tttatgtcaa tcttttttat tagtgaataa ataatataga atacaatata
       61 caaaaaagtt tcttgaaatg ctaagataat ccccatttac aaagcaacat cattatacta
      121 tgtaaatttg aatcaacaat gttttcacca tatttcacca taaaatcctc taattgtgtg
      181 tcaataaatg aataatatcc acttatacta tcgatgtatg cagtcattaa gtgtatacat
      241 tcatcacttt tatctttata catatatatc tccccctata tacaatatat aaatttagat
      301 accaaatcgt atcatattaa atagttaagt gtgtaacatc tatgcagtta tatatttata
      361 tccttatatt aataactaaa gtgagttttg acaatagatt ttgggaaact ggatgttcaa
      421 agtgcaaact tggattttaa aatcattaat taagatagtg cataagaata tatgtataca
      481 taacttaatt ataaagatta taaacaatta cttttattaa ctttatttaa gtggtagaat
      541 aacttggaga ttaataatta taaggaggat ataatatgag tttatataag tttatatatg
      601 atgataaaga atatttatta aaagaggaga actgtagtgc tttaataaat gatgaagaaa
      661 atccagttca aggagtaagt atttcaaaaa taattgatat acttaatgaa gcagaagaag
      721 ttgattttga tgtagaatat tatcaagaag catgtccatt atgtttagaa ggagtaaaag
      781 aaaagaaaaa attctttcct tttttagaat atcactttta tattttcagt agagatggag
      841 aatatgtaat aagcaatata tctattgatt ataaaggact atcttttaat aaactatcaa
      901 gggcaaataa agttgataat agttatattg taagtgtgat aatttgcgaa aattgtcagg
      961 attatatagt tcagatagaa aattgtatag tttagtgtaa agtatgtctt aatttaagag
     1021 aaatttctaa attgagacat caactttttt caaaaatagt atggaaaatt actaaattta
     1081 tgagataatg taagtcggag gtgagttaca atatatagta aatatttgga agagatagat
     1141 gatacaattt tagattcaat gtttaaacta accttgaatg aaagattatt gactaataac
     1201 gattttattg aaaataaaat tgatgtagta aagaatttaa ttgattgcta taaaactgaa
     1261 caaaatagtt taatgttatt aaaatcaaat tttttgctag ctttattata tggtattcaa
     1321 gggtttagtg aaaaaatgaa agagtatatt ttgatttcct gtaaatatat tgataaatgc
     1381 ttatccaaag attataagtt tttagcacga ttttattcac aaatagctat tttaagttta
     1441 aaaaatgaag atgtaaataa ggcaaattta tatatggata aatttaatct tatttgtgat
     1501 gaaaatgatt ttctaattga agaaattatt tttaaatcac aacttattta cattaaggca
     1561 agtaagtgta tagaatcttc tgtaataatt aaagaaatag aaacgttgta tataaagata
     1621 aaagaaatta acgattttaa ctgtaaaaaa atatattttt ttattattgg taaaatatat
     1681 tttttgttct tgaatgatgc tctaattgct aaggtgaatt ttttaaaagc aagaaaatat
     1741 gcagatttac taaaggatat gcaagtatct tcactatgta atataaagat aggtgagtgt
     1801 gaatgttcgt ttgaaaatta tgaatgctct atggaatatt tcaatgaagt aataagcaat
     1861 aaaaagtata gaaatgtaaa cataattcaa aaatatagag catcaaacaa catgactaaa
     1921 atattaataa agactagaaa ttatcctgtc gcaataaata atttagcaaa atctgaagta
     1981 tatctagaaa aaattaaaaa tcagggctta aaagaagtag agaagtttga tttatttata
     2041 agtttagcca tgtattatgc aaaaagtaat gaaaaatctt ttgaacaatc aatatgctat
     2101 ttagatagag ctaaaaacat cttaaatatg ataaaaaata tagaagaata tacaatatat
     2161 gatttagaaa taatttatca ccaagcaaag atatattata tctttggata ttatgacaat
     2221 gccttaaaca caaataaaaa actccttgaa aaatctaaaa atgctaaaaa ttataattac
     2281 gtgaagttag catacaagaa tatatatttg tgttttgaaa agattcaaaa ttatgaaatg
     2341 agtatgaaat actttaaaat gtattatgag ttaaaaatga tgtatataaa agctcgaaat
     2401 agaaattata tagattcctt aaattataga catgagtata tagaaaaaga aataaataac
     2461 atgagaaaag taaaacttga tttagataaa aaaaagtata tcgaccattt gacaagtgca
     2521 tataatagga cttttttgaa cgatttttta gaaaaacaag aagttagtga atattgcaca
     2581 gcatttatga tagatgtaga ttactttaaa gaatataatg atacttatgg acactacaat
     2641 ggagatattg ctcttaaaga tataactata ataataaaaa aatatctaaa aaaagacatg
     2701 cttatgataa ggtatggtgg agaagaattt ctgatattat ctgtatgtaa agattataaa
     2761 aaaagtaaaa tatttggaag aaaattatgt aggattgtaa agaaattttt aaacaataat
     2821 ttgacagtta gtattggcat tgatacatgt aaaaatagta gtatagatat aagagaaata
     2881 attgaaaatg ctgataaagc tttatataaa gcaaaggaaa gtggtagaaa tagatgtttg
     2941 cattatcatg attttaagaa tttttaactg ttgaaatagg attggtgatt tacttgaaaa
     3001 ggagtttaat agaagattta aagagaaata taaacaagat agaatacgat atgttgacta
     3061 aattctatct aaatttgaca gatttaaact acatcttgga ttatcacaaa gaccttttaa
     3121 atacaaaact agaagataat agtagagtct atactttata tttaattagt cttataaact
     3181 atataaaaaa agatagagaa gcatcataca actatttgat agagtcatta aagtatatta
     3241 aaaaattgtc attaaaagat gattcagata taatatctaa aatttatatt tatcttactt
     3301 tatcagctat ttctataaga aaatatgata aggcaaataa tttttatttc tcagcaaaaa
     3361 aaattataag acaaaatcat ttaaatgagt tgttggtctt gttaaatatg agtttatgtg
     3421 tagaaataag tgctttatat cagaccaaaa cagatgtaat agctttaata gaagaagtgt
     3481 ctttttttaa aactttaaaa aataaagctc ttgtaagtag ggcaaattat atatttggga
     3541 gagtttatct atatttattt aataacttta taaaaagtat ggagtaccta attgaatgtc
     3601 taaaactggc tcaagaaaat aatctatact ctatagaggc tttttgtaaa tgtataataa
     3661 gtttgtgtta tttagagagt ggaaataatg tagaagctat aaaatacata aataatgtat
     3721 tagatgttgc acacaataat aactcaatat cagtaactga aagagtgtcc ataaagatag
     3781 accttatatg ggcatattta aaagaagata tgaataaaga agccgaagct attttattaa
     3841 agactataaa attaatgaaa gttgttgaag gtgtatataa ggattattta tattcattta
     3901 tatttttata ttcagctgaa attgagttaa agaaagataa ttgtagtttt gaaaaggtaa
     3961 acacctattt aattttatca aaaaatatat ataaaagttt tggagatgtt tttatatata
     4021 attctaaact agattacata tataagttgt atggagattt atatataaaa tttgggaata
     4081 tagacgaagg tataagttgt tatataaaag gtttaaattt agttaaaaag agttcattga
     4141 atcttagaaa aatatctata ttttatgggc taatagcaaa tggatatgag ctaaaaaaag
     4201 attttaaact ggcaatagaa tattataaaa gtatggattg gtattctgat aggtgggaaa
     4261 aaaataattc ctacaaaaca tctcaagcaa ttcacaaaca atatgaactt agacaaaaac
     4321 aagagagttt aagattacta gtggatgata ataagaaatt agaaaatgat attttaaaag
     4381 atggacttac taaactatat aatagaagat atttggaaca gcagttggat ttatttaatg
     4441 gaaccagaga caaaaatatg gtcgcaatac tggtcgatat agattgtttt aaaagttata
     4501 atgataatta tggtcatcta gcaggagata aagttatagt taaggtaact gatattataa
     4561 gaagtgtatt tatagatatt accgaacaca taatcagata tgggggagaa gaaattttaa
     4621 tattagtaga ttctatagaa tcaatggatg ttgaagagat tgataatata gaattatata
     4681 ttaaaagggt tttcaaactt cttgaatttg agggtattga acatatatat tctaaaataa
     4741 aaaactatat aactataagt gctggagtaa gtataaaaag atgtcgtagt agaaaagatg
     4801 ttgagatgtt aattgaagat gcagataaga acctatataa atcaaaaaaa gcaggtagaa
     4861 atcaatacat tataatttaa aattgaggtg attttgttgc gaaaaaaaat aggaattata
     4921 gcctctgata ttgaattaaa agaaagaata gaagaattat atagagaaga tgtagaaaat
     4981 ggaactataa taatcgacat acttaaccta gacttaatgg aaaatcaagg tcgtatactt
     5041 gttgaaaaag gtgcccaagc tataattggt agaggtggag gttatagttt agtaatagat
     5101 acagtaaatg tacctgttat accaatgaat atgaagtcaa ctgacctttt aagagcaata
     5161 gaaatagcaa agaaatattc taaaaaagta gtattgattc ttggagacaa tgaagtttct
     5221 tttgactatg ttggctggag aaatgtcata agtactgaaa taactgaaga gtggtttgaa
     5281 tctaagtatg agataagaag taaagttgta aaatatatag accaaaaaga tgaagttgta
     5341 atcgtaggtg gaggtttggc atgttctttt gcaaggcaat atggaattga tagtgtattt
     5401 gcaactgcta gtgatgaatc tataagagaa gctgtagaat attgtaaaaa attattggat
     5461 acattaggtg aagaaaagtt taataatgaa gtgttacgaa atatacttga tggtataaaa
     5521 gatggagtaa ttgctataga tagtaatggt agtataattt tgtacaatga aagtgcaaaa
     5581 aacatgctta aagtagagcg aaaatgtgca cttaataaat acatcttaga tgtatttcct
     5641 aaaatggagt ggatgttaga ttgtttacat gaaaaagagg acgtagaaga tagaaagata
     5701 agaaatataa ataatctaat cgttaatact aggacaacat taataaaagt tgataatagt
     5761 acttatggag ttttaggaat aatacaagat ataaccaagc tacaaaactt agaaagaaaa
     5821 ataagatttg acttgaatca aaaaggtctc tatgctagat atacttttga tgatttttta
     5881 tttaaagata aattaactaa ggaatttatt gaagaagcta aaaaaattgg aaaaagtgat
     5941 tatacaactt tactttatgg agaaagtggg tctggtaaag aaattatagc tcatagtata
     6001 cacaacataa gtaaaagaaa agatagacct tttgttgcca taaattgtgc tactatagca
     6061 gaaaatttac ttgaaagtga actttttgga tatgaagaag gtgcatttac tggtgctaga
     6121 aaaggtggaa aaagaggact atttgaactt gctcatggag ggactctttt tttagatgaa
     6181 ataaatagtc tatcttttaa tattcagaca aaactattaa gagtaataga ggaacgtcaa
     6241 ataatgagaa ttggttctga ttatataata cctctggata taaggattat agcagcaacg
     6301 aacgaatctt taaccgaaaa aatagttatg ggtacattta gggcagactt attttataga
     6361 ttgagtagtt tagagataaa tatacctcct ttaagagaca gaagagaaga tataataccc
     6421 ttgtttaaca attttgtaaa tgaagtatta aaggatgatg gtctcaatgg aataaacagt
     6481 atagatgaaa attttgtttt gactaaagat gaaatggata agctatataa ttatagctgg
     6541 cctggaaatg taagagagtt aaagacaata gctcaaaaat atgttgtgac aggtaaaatt
     6601 aaattgagac aagatagaga ttttaaaact aaaaaagtac ctcctaactc tgaagtagac
     6661 aaatttaatt ctgaaacgac agcaagtgta gaagtccaag atgaatctat aaatataagc
     6721 aagataaatg atggtaagat aagtatagat ataaaagaag taaataagta tgttgaagag
     6781 aaaataataa gtatgctttt tgctcaggga ctttctaaaa atgaagttgc acaagttctt
     6841 ggaataagta ggacatcttt atggaagaaa tataataaaa atatttaaat ataattacat
     6901 ataaattatt ttgaagtaat gttaattttt ttaaacaagt gttaagaaaa attaacatta
     6961 cttttttgca attttttatt tttttagaca ggcatatata tttataagaa aaatagttaa
     7021 aacacttaat ttaataatga aaaaaataat aattctaaac aaaatcagtt ttacataaag
     7081 tggaatttat tggcatagta attgcttaat atataatgca agagtaaata caattttagg
     7141 ggtgatggta tgagtcaaag tatggttggt aagcatgcaa tgtggccaaa agaaaatgat
     7201 gttatattta gcatatctgg cagagctcaa gcagcagaga aggcttttgg tatggataat
     7261 gtaataaatg caacaatagg cgctctaatg gatgattcag gaaaattaat aacaatgaaa
     7321 actgtatatg aagaatataa agcattagat aactgtgaga taggtgcata tgcagctctt
     7381 gaaggtcaac cagattactt agaagcagtt aaaaaagtat ttttcagaga ttacttacca
     7441 gaaggacata taagagtatt agcttcacca ggaggaagtg gtgcaataaa attagctgta
     7501 tggaactata ctaatgaagg agatgaggta ttaacatctg attggttctg gagtccatat
     7561 gtaagtatag ctgaagaagc taatagaaaa gtagttaatt accaactttt tgatgaaaat
     7621 agaaggttta attttgagtc gtttaaagaa aaatttgtaa atatagcaga aaaacaaggt
     7681 agagtcttta caattataaa cacaccagca cataatccga ctggatatag tgttgcagat
     7741 gatgagtggg ataaaatatt agacttatca aaagaagtcg caaaagataa agacaaaaag
     7801 ataatatttt tcgtagattc agcatatatt gactttgcag gtgatgatga tgtatgtaga
     7861 aagtttttta agaaattttc taatttgcct gaaaacgttc tcgtattggt tggattcagt
     7921 atgtcaaaag gatttacagc ttatggtatg agaatggggg ctataatttg tatatcttca
     7981 agtgaagata tagctgaaga gttccattac tcctgtgttc attcatgtag agcaaattgg
     8041 tcaaattgta atagaagtgc catggcagta ttatctaaca ttgtaaatga ccctaaaaaa
     8101 ttcaaagaat atgaggatga aaaagaaata tacaaaaaca tgcttactag aagagcagat
     8161 gtatttgtaa aagaagctga aagagtagga ttagaaattc ttccatatat agcaggtttc
     8221 tttgtaagta taccatgtga taatccaaaa gaagtatgtg aagaattgac taaacataat
     8281 ttatttgcag ttccattaaa aatgggctta agatttgcag tttgtgcagt ttctgaagat
     8341 aaatgtaaga aagcaccaag tattataaaa gaagcacttg aaagtttaga agtaaaaata
     8401 aataattaat ttcattgaac aaattgggtg gactatgaat ttaataattg tagatggagg
     8461 gaattttata tgaaacaatt gatgacaggt aatgaagcaa ttgcacgtgg agcctatgag
     8521 gctggtgtta aatatgcatc tgcatatcca gggactccaa gtacagaaat attagaaaat
     8581 atagcaacat ataaagatgc aatagttgca gaatgggctc caaacgaaaa ggtagcactt
     8641 gaggctgcta tagggggttc aattgcagga gcaagaacaa tggcttctat gaaacatgtt
     8701 ggtttgaatg ttgcagctga tccaatattt acttatgcat atacaggggt aaatggtggt
     8761 atggtactta ttacagcaga tgagccggga atgcattcat ctcaaaatga gcaagacaat
     8821 aggatgtatg caaaatttgc aaagatacct ttatttgaac catcaactag tcaagaagct
     8881 aaagatatga taaaagaagc ttttgaagtc agtgaaaaat acgatacacc agttttatat
     8941 agagttacaa caagactttg tcattcaaaa ggattagtgg agtgctatga tagagaagaa
     9001 gttgaaatta aagagtatgt taaaaatgct aagaaaatgg taactgttcc agcaaatgct
     9061 caaataagaa ggggcgttgt tgaagaaaga atggaaattc ttaaaaaatt ctcaaatgaa
     9121 actgatttaa actactatga aataaatgat acaaaaatag gtgttatagc ttctggtatg
     9181 tgttgtaatt tcgccaaaga agtttttgga aaaaatgcat catatatgaa actaggattt
     9241 acaaatccat taccttatga aaaaataaaa gaatttgcag aaaaagttga taaaatatat
     9301 gttatagaag aaaatgaccc atttatagaa gaacaaataa aagcatatgg aatagattgt
     9361 attggaaaag atgtaatacc accatatgga gaaatgacac cagatgtttt aagaaaagct
     9421 atatttggaa aaacaaatga tactatagaa tacaaatctg aattagtaac accaagacca
     9481 ccaagtttct gtgcaggatg tccacataga ggattcttct atgaacttgg aaagagaaaa
     9541 aatttaatag ttggtggaga tataggttgt tatactcttg gatttgctcc tccatataat
     9601 ggaatagatt atgttgtttg tatgggttct gcttttggaa ctgcacatgg agctcaaaaa
     9661 gttcttaata tgaaagatga taatgaaaaa agattggtag gtgtacttgg agattcaaca
     9721 ttcttccata caggaataaa tggactttta gatgttgttt acaatagagg taattctatt
     9781 tcagttatat tagataatag aataactgga atgacaggtc accaagaaaa cccaggttca
     9841 ggatatactt tacaaggtgc taaaactaaa gaagttgata tagaaggatt agttaaagct
     9901 tgtggaatag aacatgtaag agttataaac cctaacaatt taaaagaagt aaatgaagca
     9961 ctagattggg ctttagctat agaagatgaa ccatcagtaa taataacaag atggccatgt
    10021 gtacttaaaa aattctctaa agaagatata gaagaattta ataatccatt taagactaag
    10081 tgtaaagttg accatgataa atgtatagga tgtaaattat gtttgaaaac tggatgtcca
    10141 gctctatcat ttgataaaga gaataagtta tcaaatatag atagaaatca gtgtgtagga
    10201 tgtggagtat gtgcacaagt ttgtccaaaa caagcaatag ttaaggagga aaaataatat
    10261 gactaagagt atacttttgg taggtgtagg aggtcaagga actatacttg ctagtaaatt
    10321 attaactatg ggattgatgg aagctggata tgatgtaaag atgagtgaaa tacatggaat
    10381 gagtcaaaga ggaggttcag tttcctcaca agttagatat ggagattgtg ttcattctcc
    10441 agttatagaa attggtggag cagatatatt agtatctttt gaaaaaatgg aagctttaag
    10501 atggttcaat tatttaaaac cagaaggaaa agccttagta aataatcata ggatagattc
    10561 tatgactgtt ttaatcggag gagctgagta tcaagaaagt gatatagatg cagagttaag
    10621 tagattaaat gcaaaagtta taaatgcagc tgataaagct gaagaactag gaaattcaaa
    10681 aataatgaat gttattttat taggatgtct agttaaatct atgcaattag agtctattga
    10741 ttgggaaaaa attataagtg aaaatgttaa accaaaattt gttgaattaa acataaaagc
    10801 tttccatgaa ggtatggaaa tggttagcaa atagtttata atagatataa atagactata
    10861 gaaattaagg tgaaaggaag aaaattatga cttacagaat attagccata aatccaggtt
    10921 ctacttctac aaaaatagca gtatatgatg gagaagaaca aattcttgtg aagacgatag
    10981 accatccggc tgaagagatt gcaaaataca atactataca agaccagttt gaaatgcgta
    11041 aggaagcagt tttgaagatt cttaaagaaa atagtataga cttaaaatct cttagtgcaa
    11101 tagtaggaag aggtggagtt ttaccaccag taaaatcagg agcatattta gtaaatgaag
    11161 aaatgattga tgtactaaga catagaccag tacttgaaca cgcttccaat ttaggtgctg
    11221 ttgtggcaca tgcaatatca gaacctcttg gaataaactc atatatttat gattctgttg
    11281 cagtagatga gcttatagat gtagcgagaa tatctgggct ttgtggaatg gatagatcaa
    11341 gtgcagggca tgcattaaat actagagcaa tggctttaaa atatgctaag gataaaggaa
    11401 aagattataa gagcttaaac ttaatagtag ctcacattgg tggcggagta agtatttatc
    11461 ttcatgaaaa aggaagaatg gttgatatgc tatctgatga tgaaggacca ttttctccag
    11521 aaaggtcagg aagagtacct gctacaaaat tagtggctgc ctgttattca ggtcaatatt
    11581 cagaaagaga aatgactaaa aagataagag gtaaaggtgg tatagtttca tacctaaata
    11641 ctgtagatgc tagagaagtt gaaaaaatga tagcagaagg aaatgaagaa gcaaaaatta
    11701 tttatgaagc aatggcttat cagttagcaa aaggtattgg agagttagca actgtagtag
    11761 atggaaaggt agatgctata attataacag gtggaattgc atattctgaa atgtttactt
    11821 caatggttaa aaagaaagtt gagtttatag caccagtaga aattatggca ggagaaaatg
    11881 agttggaatc acttgctttt ggaactttaa gagtactaaa tggagaagaa gaagctagaa
    11941 tttatagtga aaattaataa gaaaaactaa aaatgtttat atactaaaaa agtggtatat
    12001 ccgtttttta tacacgaaag cttagagtat aggtgaaaat gtataaaatt tggattgcca
    12061 ctttttataa ttaaaatttt atcttaacct aataaataat tctgtaaaat ttcgtattta
    12121 tatatatatt ttattggtat ttattgttat tattattatg aggagtgatt tttgatggga
    12181 aaaataagtt taaaaagtat gataagatta ttttcaggtt tttttatatt tgcaatttct
    12241 tctgtgctta tgattaatgc acatgtaggt cttatgcctt gggatgtact tcaccaaggt
    12301 ttatctataa agctaggtat aactataggt caggcttcta taatggtagg agttgtaatt
    12361 gttatattgg atgcagtttt tggtgaaaat ataggatggg gtactttact caacatgaca
    12421 tttataggaa tttttattga tttagtaata ttttctggag tgatacctca tgcaaacaac
    12481 acttatatag gtgtgtttat ggttgttata ggaattattt tggcagctat agcaagtttt
    12541 ctatacttag gtgtttgtct aggaagtgga ccgagagatg ggcttatgat tgctcttcag
    12601 aaaaaaacta ataaatctgt acgtcttgtt agaacaattc ttgaaatact agcattagtt
    12661 gttgggtggt tattaggagg ttctgtaggt attggtacgc ttgtaagtgc tttaggttta
    12721 ggatatgtat tacagatagt gtttagaata tttaaatttg acacaaagtt gctgaaacat
    12781 agatttatta tagatgatat aagagaatgg aaggaaaaga aaaacgagga acataaatgt
    12841 aaatcaagta ttgtaataaa aaatgagcag aattaattac gttaaaatat atttgtagtt
    12901 tatctaattt atagtaaagt caaggagatt ttgaaatgga attatgggat ttatataatg
    12961 ctgatggaat taaaacagga aatgttataa aaagaggtaa ttctattgaa gaaggttatt
    13021 accatttggc agttgaagtg tggattttaa atagtaactc tcaaatttta attcagaaaa
    13081 ggtctaaaag taaaaagaca ttgccaaata tgtggggaat gacaactgga tgtattgttt
    13141 ctggggaaga gagtttagaa ggagcaattc gcgaagcaaa ggaagaaata gggatagata
    13201 taactaaaga tgagatgaaa gtttttagaa gtatgattca tgaagatact ctttgggatg
    13261 tatacttagt taaaaaagaa tatgatatat ccaaagctat tttacaggag gaagaagtaa
    13321 gtgatataaa gtgggtaagc acagatgaaa taagacaatt actaaaagag ggtttgtttt
    13381 ttgaatatcc agagatatac gaattgctat ctgagataga taataataaa ttaaatatat
    13441 gatgtagagc agatttcagt ataagtaaga ttatttaaaa tataccttct ctaaatttta
    13501 atattgtttt atgaaaatta ataagttatt tttagtattt ttctacttgt tttttgagat
    13561 taaaataaaa agggcagtca ttataaaggc ataaaaattt ggggaggatt caaactatgg
    13621 aagataacaa aggattggca cgcgatagtt caaaatataa aattgtttct atatctgtcg
    13681 tagtaattat tctaattaca ttttggtata tgttaaatat ggtgctttta acatttatta
    13741 tgacttttat tttctataac ttgcttgtgg ccacaagaaa acgaattaag aagttttctt
    13801 ctttaaatat tccagattca ttgataatta tagtattata tgcactattt gctatattgt
    13861 tagtattgat tagctatgca gttgtaccaa taattatagt tcaacttaca gagttgagta
    13921 gggtttttag tgattttgat gtaaatcaat ttgctcaatc tttaggacca aaattgtatc
    13981 ctattgtttc aaagctggat tttaataaat atatttcaca agcagggcta cttatagcat
    14041 caacagcaac aaaagttgga agttttggag taaatatatt attggcattt ttactgagtt
    14101 tacttctttt acttgaaaaa aatgaaatta aaaattttgg agataaactt tctgatagta
    14161 agatttcttt tatttataat tcgcttgtat tttttggaaa gagctttgtt aaaaattttg
    14221 gtgaagtaat gaaagtacaa gttatgattg cctttataaa ttctgttgtt tctatgattt
    14281 ttttaggttt catgggattc cctcaaatat gggctttagg atttatgatt tttgtgttgg
    14341 gtcttattcc agtagcagga gttatagtgt ctttgattcc attaactgta atagcattta
    14401 atacaggagg aataacaaag gtttttggag ttttacttat gatttgtata gtgcatgcag
    14461 tagaaacata catattaaac cctaagttga tgtcgaatag aacgaaactt ccagtttgtt
    14521 ttgtttttat tattttactt gtaggagaac attatttagg tgtatgggga ttactaattg
    14581 gagttccaat attcatgttc cttatggata ttctgggtgt taaatttact tctaggtaat
    14641 atattaaaat aataatttgt ttttatatgt aaattatgaa ttgttttaaa aaagaaccta
    14701 taaatagaca tcattaataa agacatctat ttgataggtt caattttact ttttataaat
    14761 actataaaat taaataaaaa gaaatttatt tcatttcaaa agtttcacaa cttgcatttc
    14821 tttcatttat atgaactttt tcagctttgc aaagttcatt tttattgtat ttacatttta
    14881 cagcttcaca atgaatgtta tctgtatcaa cttggttagt ttcattagaa cgactagtta
    14941 taccagatgt atatttgtct tcaaaagaag cacaataagt attttctgta gtagttgctt
    15001 gcattccatc tacttttata ctaccagcat aacaatatcc actagtatta taagcacaat
    15061 tagtagcagc acaatttaaa ttattatttt tcataaataa cctcctaagt tttatttaaa
    15121 cttattatgt gaagttatct aaattttata cacttattta caattacatt tatcacattt
    15181 aacaactaca tctttggaag tttcccttcc taaattttta ttaacatgat acacatcaac
    15241 attttcagtt gatccacata tagaacattt aaaattgttt tctagtaggg ttctttcttt
    15301 aaataacttc caatacatag aattgatgta atcattataa tttaccttga ggcatgtctc
    15361 aagaaagttt atgtatagat tgtctaaatc tcctgtattt aactctgtat attctggttc
    15421 aaaatattca caacatcctc taaaatcagg ttttgtatct gaaataacac atctaatatc
    15481 atatctatag tctaggctag gccccttaaa tgaaagatgt gtgcaaaaag aacaagatgt
    15541 ttctggtaga tttttttcaa gttcatgtaa tttattattc ataagtattc ctccaaaaaa
    15601 tttattttaa gtatataaat gaatagatta ttttaaaact tttatttatt tatattagag
    15661 atttaggata aatattccaa taaggagtaa atacttaagt atttattact gtattaaata
    15721 aaaaagaggc aaatctaact tgagattgcc tactttttta tcatttttta ataaaactta
    15781 gtataagtat tattttaatt agatttagtc agccaattta gtatctgctt tgctttttaa
    15841 ctttctacca gagcgtacag ttaaaatagc atataataca gtaaatatgg cagctaaaac
    15901 atatgatata tttatgttta agttaaatcc aatttgagca ttaaatatat aactgaatat
    15961 aacaaaagaa taaaacatac caggaattaa gcttataata taattctttt cttttattat
    16021 aaggtaaact gtaatcattg caaacgcaaa tatggcaata gtttgattac tccaagagaa
    16081 gtatctccac aatatattaa atccagaagc actaagcttt gcaaatataa ggatggctat
    16141 gacaggaata aatatacaga ttgacaatat aacacgatgt ttcttttcct tttggtcata
    16201 atgaaagtaa tctgcaagca ttaatcttaa agatcttaaa gctgtatctc cagaagttat
    16261 tggtaaaaca attacaccta ttatagctat gataccacca atagaaccaa gtaaatctct
    16321 tgcaactaga cctattacat caggagaacc tactaattcc ttaggtattc ctttattgta
    16381 aatacccata gcagctgcag cccaaatcat tgcaattaaa ccttctaaaa tcatcatatt
    16441 ataaaaagta gtttttcctt ctttttcatt tgaaactgat cttgctataa gagtagcttg
    16501 agttgagtgg aatccagaaa cgataccaca agcaactgta acaaagaatg taggaataag
    16561 aggaatacca tcaggatgaa tacctttcca atttgcaaga cttaaattag ctaaatcata
    16621 accttgtgta aagattccaa ttccaacacc agcagcagat aaaagtagaa tagctccaaa
    16681 tattggatat atctttccta tgattttatc aattggaaat agtgttgcac acaagtaata
    16741 tagaaagata agtccataaa ctatccacac aactggatta tttatagtcg atttcatatt
    16801 taaaatttga gtaacaacta aatctccagg tgtataaata aaaactgcac cgactaaaag
    16861 cattaaaaga caaaggaata tattatagac ctgaaatact ttattaccta aaaatctact
    16921 gataagagaa ggcatttgtg ctcctttttc tcttatacta atcattccac tcatatagtc
    16981 atgtaaagct cctccaaata cacagccaat aggtattagt atgaatgcta ttggaccaaa
    17041 caatatacct tggataggtc ctagaattgg tcctgtacca gctatattaa gcaactcaat
    17101 tagactattt ttccattttt tcattgggac atagtctact ccatcacttt gagcaagtgc
    17161 aggtgttttt ctgtcatctg ggccaaatac tttttcacaa taagcaccgt ataaagcacc
    17221 tcctattagt aatatagcca agccgattaa gaacgttgtc ataatatcac cccatttata
    17281 aaattttttg gtctttgtat acatattatc taaatgtgta gatttatatt gaatttaatt
    17341 ctgcacaacc tacaaattct ataaagtatt acaactaata taatttcaga aataaataca
    17401 tatacataac catgtactta gtatacaggt atttggtaat attttcaatg ttatagcaat
    17461 aaagaatata aacaaaattc tgataatatt ttacaaaaaa attaaaaaat ataaaaatta
    17521 gtttaaatag atggggaaaa aaggaaaatc aattgagaat ataaatgtct atatcttaac
    17581 aatattaaga ggtttaaaat tcttgaagaa agaatagtca agtgatataa tgaatttaca
    17641 ggtgtatata cactagtaca tatagaaagc ggtgcaatta tggataaaga tttaacatat
    17701 caaatcaaag agctaaagaa agaaaaaaat gcaataatac ttgcccattt ttatcaacca
    17761 ccagaaatac aagaattagc agatgcagtt ggtgactcat attatttaag tgagattgct
    17821 agagattgca aagaagaagt ggttgtattt tgtggagtaa ggtttatggg agaaagtgct
    17881 aagatattat cacctgaaaa aactgtattg atgccagtaa gtaacgctgg gtgtgctatg
    17941 gcagatatgg tagatgaaga aggcgtgata aaattaaaac aacagtatcc aaatgctttg
    18001 gttgtatgtt atataaactc tacagctaaa gttaaggcac attgtgatgt ttctgttaca
    18061 tcttctagtg caattaaaat actagaaaac atagataata aagaaattat attccttcca
    18121 gataaaaatt taggaggata tattgcagaa caatttccag ataaaagctt tatattttgg
    18181 gacggatatt gtaaatacca taacaatata agagctgaag aaataattga gttaaaagat
    18241 aagtataaaa atgcagaggt attagtacat ccagaatgta aaaaagaaat aagagattta
    18301 ggagattatg taggtagtac atcaggaata ataaaatatg ctactaatag taaaaacaaa
    18361 gactttataa tagcaactga agaaggtata ttacatgaat taaagaaaaa taatcctaat
    18421 aaaaactttt atattcctgg tggaaaaata ctatgtacag atatgaaaaa gacaacatta
    18481 gaaaatttat atagtacact taaaaatatg gaaaatgaag ttattgtaga agatgaaatc
    18541 atggaaaagg ctttaaattc actgcttaat atgcacaaat tagcagaagg atagagggat
    18601 agacttatga atttagaaca agatgtttta atagttggaa gtggagtttc aggattatac
    18661 tgtgcattaa atttagataa aagtttaaat gtgctagtag tatcgaaatc aacaattgaa
    18721 aacaataata catatcttgc acaaggtgga atttcaactg ctagaaatat tgatgatata
    18781 gaatcctttg tagaagatac aatgaaggct gggcagtata aaaatagagt tgaagcagtt
    18841 caagtacttg cagatgaatc tatagaaaat gtagggcaga tagtagaata tggtatgcct
    18901 ttagacaaag aaaatggaga gatagattac acaagagaag gagcacatag tgtaaatcga
    18961 atagttcata gtaaggataa tactggagaa gttgttttta aaactttatt aaaagaagct
    19021 aaaactagag aaaatataac tcttatagaa gatgcctatc ttttagatat attaaaagat
    19081 ggtaataaat gtataggagc tagaattttt aaatcgaaaa aagagattca tgttttttca
    19141 aaaatagtag ttttagctac aggtggaatt ggtgggcttt ttaaaaattc tactaatcaa
    19201 agacatctta caggtgatgg aattgcaata gctttaagaa ataacataaa aatagaaaat
    19261 ttagaatata ttcaaataca tcctacagcc ttttatgaag aaaataatga aggtaggaga
    19321 atgcttatat ctgaatcatt aagaggagag ggagctaaac ttttaaataa aaataaagaa
    19381 agatttgtgg atgaacttct tcctagagat gtagtttcaa aagctatttt tgaacaaatg
    19441 gaaaaagata aattacctta tgtgtattta gatgcaacac atttagatag tgaatattta
    19501 attaatagat tttcttttat ttataatgaa tgtttagcta gaggaacaga tataacgaaa
    19561 gaatgtataa aggtatctcc tgcacaacat tattttatgg gcgggataca tgtagatttg
    19621 gactcaaaaa catctatgga aaatttatat gcagttggtg aaattagttg tacaggtgtt
    19681 catggagcca atagattggc aagtaattca ttacttgaag ggcttgtatt ttcaaaaagg
    19741 gcagctaaaa atataaactc agttatagat aatgtaaaag ttaagtttat agatgtacca
    19801 gatatggata tagatataga acaggtaaaa aaagaaaata aaatattagt cataaaagaa
    19861 attgaaagaa caagtgagga ttttggcgat gaattatttg attattgata gaatgataca
    19921 ggatgctttg atagaagatg ttccaagtga agatataaca actaattcta ttgtggatga
    19981 aagttctaaa tctactgtag atttaatttg caaacaagat ggtataatag ctggcttagg
    20041 tgtatttaag agagtttttg agatacttgg tgatgtagat gtaaaattat ataaaaatga
    20101 tggtgataga gtaaagaatc gtgaaaagat agctgtatta acaggtagta ctagaaactt
    20161 acttgtagga gagcgtgtag cattgaatta tttacagaga atgagtggta tagctactat
    20221 tacaagtagg tatgttgaaa agcttgaggg tacaaataca aaattattag atagtagaaa
    20281 aacgatacct aatcttagaa tattagataa gtattctgtt aaagtaggtg gaggatgcaa
    20341 tcatagattt aatttatctg atggaatatt gcttaaagat aaccatatgg gggctgctgg
    20401 tggtgttaag aacgcagttg aattagctag aaaaaacact tcttttgtta gaaagataga
    20461 agtggaagtg gaaactttag acatggtaaa agaagcaata gaagctaatg cagatataat
    20521 aatgttggat aatatgagtt tggaaatggc aaaacaagca gtagatatta taaatggaag
    20581 agcaattgta gaattttctg gaaatgtaaa tttagatact atagaagaaa taggaaaaat
    20641 tggtgtagat gttgtttctg taggagcgct tacacattct gttaaagctc tcgatataag
    20701 tatgaagaat ttgagaaata tataaatttt atattgaaat aaaattaatt aaaatagact
    20761 ccttaaattt gttttataaa tttaaggagt ctatttttta ttcattaaaa ttaataaaaa
    20821 tagaaaaaaa tcaatcaata atttaataaa aatagataaa tactatatac acttttaata
    20881 atatagttat tatctatttt acagataaat aagaataaac ttataatata attgtatctg
    20941 taaaattttg taaaaatata aaaagtatag ggggaataaa aatgaaagta gcttatattt
    21001 ttagtagtac taatacacat aaaattctag acaaaatgat aataccgcaa cttgaacaag
    21061 gaactcatgg agtagacgtt ctagggatgt ttttctttat ggataatacc ttatttttat
    21121 ctaaaggtaa tcctgtaggc gagagactaa gtaaattaca tgaaaaaact gatatgataa
    21181 taatggcttg tgaccaatgt gctatagaaa gaggtataga gaataatttg gttgatggag
    21241 ctacaattgg atgtttccca aacctatatg cttgtttagg cagtgcaggt gtggaccaag
    21301 ttataacgtt ataaattagt ataggataca atccatagaa aaatttattt tctgtggatt
    21361 attttaagta aaagataaat tgtttaaagt aggggatgaa caaatgtcta aaattttagt
    21421 tgaatttata aacacttgac catcttgtga tgaacatgga gtgtttataa aaaaaatgtc
    21481 agaaaagtat gctgataaat tggaaataaa attatatcaa gcaggtaaag atttttcgta
    21541 tataaaaaaa tatgggatta taacaaaagg aactttaata ataaatcaga aaaagaaata
    21601 tgatagatta aataaagata caattgaaag ggcaatagta gaagctataa acaataatta
    21661 aaagtttaaa aattaggagt atcaaaaatg aatatattag aaattgtttt aaatggagtt
    21721 aaagatacta tagtagcttc tatagttaca ttgaatggag catcaggatg gcttatgata
    21781 agttttatat tggcaggtgt acttcacaat gttttaactc caagaaggtt tcaaaaacaa
    21841 cttggaaata agaaattttc ttctttaatt aaaagtacta tctcaggaat gtttttacca
    21901 atatgtagtt gtggagtaat accacttgga ataagtatgt attattcagg agcatattta
    21961 ggaccagtac ttgcttttat gacatcaact ccaataataa atccaatagc tgtagttctt
    22021 tgtctaggtc ttttaggtcc taaagttaca atcatttatc taatagctgg ttttttagta
    22081 ccatttttag taggattttt aggaaattgt tttggaaaag aagaattatc acttcataat
    22141 gattcagagg aagaagtaat agaattagaa gaagaaaaaa gaagttttat ggaaaatatg
    22201 attgatggtc ttaaatggtc ttttggggag ttagctctta ctattagtaa atatgtagta
    22261 atgggtatgg tttttgcagg atttataaca accatttttc caaatagtat aattcaaaaa
    22321 tatttaggaa atccaggttt gatatctctt ggaagtatag caatacttgc atgtgttatg
    22381 tatgtatgtg ctgtaggaca tataccgttt atagcagctc ttgttgcaag tggagcatct
    22441 cctggtatag cgataacatt tctaatagct ggagcagcta caaatctacc agaacttata
    22501 agtatgttca aacttattgg aaaaagaact gttattatat attctgtaac tttgacactg
    22561 tcttcaataa taattggata tataacaaat ttacttttag tgaataataa ctcagtaaat
    22621 ttagatgcaa caaattcttc aatacagttg gcaaataaac taatgttaaa tatacctgat
    22681 agttttgagt atgtatgtac tattttaatt attttatttg catttaaatc aataatacca
    22741 aagatgaaag aggttatagg aaatcatgct taaaaagata gttataatat cctgtatagt
    22801 agttgtgtta atcattttat caaaaatagt tgatgataac atcaaagaag atgcctctat
    22861 tccaaatgtg aataaagaaa cattagaata ttttagaaaa aattataaag aagacataat
    22921 aacttgtgct gaagaagact taaataatga tgggaaaaaa gatttagtag ttatatataa
    22981 aaaaagcaat aattcaaatg aaatggtagt cgtagtttct gataaaaata gccactatat
    23041 aacaaagcca ataccagcac ctatagagaa tcaaactata acctttaaaa atattgatga
    23101 taaagtacca atagaagtaa tagtatcagg gtctaaaaat ggtaatgtag gatatgctat
    23161 ttacagagta gagggtaaga agtttgttga tttatttggc gaagatatgg acaaatgttg
    23221 ctaaaaataa aaataataca taaaggtggg aaatttatat gaaattatct aagaagattc
    23281 aagcattaat cttatttggt ctaatgggta catctatatt aacaggatgt agtagtaaac
    23341 caaaagaaaa agaagaagca aaagctggaa aggatgatta tacaataaca gtaggttatt
    23401 ataattgtga ccatatgact gcgggaccag tagcagaatc ggcaggaata tataaagatt
    23461 taggtcttaa tgtaaaaact gttggaaatg gtaaagttcc tgaagctatg gcagcaggaa
    23521 aaatggatgc aggttacata ggaactaaag gtttagttgg agcgatacct aagggttcac
    23581 caataacaat agcagctaac aaccatactg gtggttctga atatttaata gtttcaaaag
    23641 atataaagga acccaaagat ttaattggta aaaaaattgc aacagatatg agtgatttct
    23701 tatggacaag tgattatggt ccagaaacag gtctaccaac tgacccatca aaatatgaag
    23761 ttgtaaatat ggattcagac aaagataaat atttagctct taaaactggg aaaattcaag
    23821 cctttacatc ttgtgaccct tggggttctg tagctgaaaa tgatggtgca ggaaaaataa
    23881 tagcatctac tcaatataaa gaaaaagtta atggtaaaga atataactgt tgttcattct
    23941 cattaaacaa aaactttata aaagaacatc cagatttagc aaaaaaattg gttctagcac
    24001 atacaaaagc aatagaatat atatatacaa atccagctga agcagctaaa atatttgcaa
    24061 aatattataa tgtagaagaa gaagttgcac ttagaactat ctataagaag actgttggag
    24121 aaggtagaac tcttacttgg aaagttacag gtgaagaata taaaaacaat ttacaaatgt
    24181 ataaagattt aaaggcttta gatgatatac ctaaatatga agatacgatt gatacaagct
    24241 tattagactc ttgtggagct gatgactttg acaaatttat aaaagaaaaa gttaacccag
    24301 agtttcctga aggtatgagt tatgaagact ggaaagcaaa ggtattataa ataaatggag
    24361 aatataaaaa atataataat actttttatc attattttgg gaagtatatt tataatattt
    24421 tttaattttg aagataaaga agaaataaaa ggaaaactaa gaataggggt atctgatgat
    24481 acctctggtt ttgtaataaa ttatatggta aatagagact actttgaaaa tataaaagta
    24541 agtgatataa tggatatttt tacaattaat gattgctgag caagtacaat gcagtgggct
    24601 ctgagttcag aggaaataga tatagcaatc atatgtaaag atgctgcaaa acaatatgta
    24661 aatgtaaata gtggatttga aattgttggt actgtagttc aaaattctga tatatttcta
    24721 ataagtgata ataatcctaa aaagatggga gtcattcaaa atagagatta ccagtcagaa
    24781 ttggttaaaa aatactacaa gaatgtggaa gtagctccct tattagggac tgcactagca
    24841 tatggattag aaagtaattt agtagatggt gttgtaattg attgcataaa gtcaattggg
    24901 ttagagggta aaagaaaatc tacaactaaa ttaggagatt atgataccta tgtattagtt
    24961 gtaagtaaag agtttaaaaa aagtaaagca tatagtgatt ttataacact ttacaataaa
    25021 agtgttgatg ctttaaagaa taaagctaca tttaagaaag cattaaaaga gtacaggaat
    25081 ataagaaata tagatatgga ggaaataagc aattggaaat taaaatttct aaagataaaa
    25141 gcgatagatt aactaagaaa aaaactgatg gtagtatgga tattttatat aaaataatac
    25201 tattagttat tatattttta gtctggcatt ttgcagcaaa agatataggg agttctttat
    25261 tacttccaat gccagtagat gtaataaaag gtttcttttt ctgtgtgaca gatgcagaga
    25321 cagtcactaa tctttttata acattgcaga gagtacttaa aggttttatg tatgcattat
    25381 tgtttgggct tccaatagga tttattatgg gattttctaa aacttttgaa agggttttat
    25441 ctccagtagt agattctgta aggcaagttc caataatggc atgggttcct cttactatag
    25501 tatggtttgg tataggagat ggtccaacaa tatttttaat agctttttca ggggttttcc
    25561 ctataatttt aaatactatt caaggagtaa gagcaatatc aaaggattat tataatgcag
    25621 ctagaagtat gggagcatcg ccaattgtga tatttacaaa tgtaattgtt ccagcatctc
    25681 ttccagatat actaactggt tcaaggattg ctataagtac tggatggatg tctgttatct
    25741 gagccgagtt tatagctacg agtgccggtc tcggttactc aatggtacaa gctcaaacat
    25801 taatgcatac agatgtatta attgggctta tgatatttgc agctttaata ggatttataa
    25861 tagatagggt attaaagttt ataaataaaa tcctttgtag atggaggttt gcagattaat
    25921 gaagttaagt gtaagagata taaataagac atttgtaaat aatagagtac atacaaaggt
    25981 tttagaagat ataagtatgg atatagatga tggacaattt gtctgtcttt taggaccatc
    26041 aggttgtgga aaaacaactt tacttactat aataggtggg tttcaaaaat cagaacgtgg
    26101 agatgttttt ataaatgata aaagagttaa aaaaccaggc atagatagag cttttatatt
    26161 tcaaaattat gctttatttc cttggaaaac tataagaggt aatgttctat ttcctatgaa
    26221 acagcagaaa ataccaaaag aaaaaagaga agaaatgcta gaagaacttt tggttatgtc
    26281 agacttgaaa ggaaaagaaa aactgtttcc acatcaatta tctggaggaa tgaaacaaag
    26341 agtggccatg ataagagctt tagcatgtaa tccagaggta ttactaatgg atgaaccatt
    26401 aggagctgta gattttcaaa tgagacaaaa tttacaggaa gagctagaac gaatatggat
    26461 aaagaagaaa ataactgcat taatggtaac tcatgatgtt gatgaagctg tatatatgag
    26521 tgatagagtt atagtaatgt cgagagataa aggaagaatt attgatgata taaatataga
    26581 tattccaaga cctagaatta gaggaagcca aaaatatgaa gagtacaaaa acaaattaac
    26641 tgatacttta tcaaagtgtt atgaagttta aagaggagta gacatgagtc aaatagaaaa
    26701 atacaatagt ttggataata cacaacagaa aataagaatc tctattatgg atatgataat
    26761 agataaaggt tctagtgtta cacttcaaga agtgactgaa tatataagta aaaagttgaa
    26821 tatagaaaaa gaatatatag aaagtacact acaatatttt attgataaaa atataatggt
    26881 tgttgatgga gatagtataa attttatata tcctgtatca gcattgccaa caaatcataa
    26941 agtaactttg agagataata gaagtttcag tgcaatgtgt gctatagatg ctataggaac
    27001 ctcttgcaca ttcaaccaag atataaaaat aaactctatt tgtagtgcta caggtaagga
    27061 aatagaagta attataaaaa atgaaaagat tgagtatgtg aataatccga acttaagagt
    27121 gcttcatata aatcttgata agcatctaaa ttgggctgct agttgctgaa atataatgaa
    27181 tttcttctgg acgaagagtg attttgatgc ttggacaaac gaagcgggat tatctgatga
    27241 tgaagatata tattgtttag atattaatga ggcaatagta gaatcatata aaatatttaa
    27301 actcaaac
//
LOCUS       DAEMAE010000031        26910 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEMAE010000031 DAEMAE010000000
VERSION     DAEMAE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.031"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(41..814)
                     /locus_tag="KPH93_001060"
     CDS             complement(41..814)
                     /locus_tag="KPH93_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG1687894.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(814..1200)
                     /locus_tag="KPH93_001061"
     CDS             complement(814..1200)
                     /locus_tag="KPH93_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687895.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1225..1542)
                     /locus_tag="KPH93_001062"
     CDS             complement(1225..1542)
                     /locus_tag="KPH93_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG1687896.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1554..1814)
                     /locus_tag="KPH93_001063"
     CDS             complement(1554..1814)
                     /locus_tag="KPH93_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG1687897.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1814..2110)
                     /locus_tag="KPH93_001064"
     CDS             complement(1814..2110)
                     /locus_tag="KPH93_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1687898.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2179..4605)
                     /gene="gyrA"
                     /locus_tag="KPH93_001065"
     CDS             complement(2179..4605)
                     /gene="gyrA"
                     /locus_tag="KPH93_001065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG1687899.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4625..6526)
                     /gene="gyrB"
                     /locus_tag="KPH93_001066"
     CDS             complement(4625..6526)
                     /gene="gyrB"
                     /locus_tag="KPH93_001066"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG1687900.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPATARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6527..7642)
                     /gene="recF"
                     /locus_tag="KPH93_001067"
     CDS             complement(6527..7642)
                     /gene="recF"
                     /locus_tag="KPH93_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG1687901.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7660..7866)
                     /gene="yaaA"
                     /locus_tag="KPH93_001068"
     CDS             complement(7660..7866)
                     /gene="yaaA"
                     /locus_tag="KPH93_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG1687902.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8003..9109)
                     /locus_tag="KPH93_001069"
     CDS             complement(8003..9109)
                     /locus_tag="KPH93_001069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG1687903.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9351..10673)
                     /gene="dnaA"
                     /locus_tag="KPH93_001070"
     CDS             complement(9351..10673)
                     /gene="dnaA"
                     /locus_tag="KPH93_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG1687904.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11329..11466
                     /gene="rpmH"
                     /locus_tag="KPH93_001071"
     CDS             11329..11466
                     /gene="rpmH"
                     /locus_tag="KPH93_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG1687905.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11544..11888
                     /gene="rnpA"
                     /locus_tag="KPH93_001072"
     CDS             11544..11888
                     /gene="rnpA"
                     /locus_tag="KPH93_001072"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG1687906.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11920..12171
                     /gene="yidD"
                     /locus_tag="KPH93_001073"
     CDS             11920..12171
                     /gene="yidD"
                     /locus_tag="KPH93_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG1687907.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12203..12910
                     /locus_tag="KPH93_001074"
     CDS             12203..12910
                     /locus_tag="KPH93_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG1687908.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12879..13508
                     /locus_tag="KPH93_001075"
     CDS             12879..13508
                     /locus_tag="KPH93_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG1687909.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKEEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13620..14999
                     /gene="mnmE"
                     /locus_tag="KPH93_001076"
     CDS             13620..14999
                     /gene="mnmE"
                     /locus_tag="KPH93_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG1687910.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15094..16989
                     /gene="mnmG"
                     /locus_tag="KPH93_001077"
     CDS             15094..16989
                     /gene="mnmG"
                     /locus_tag="KPH93_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG1687911.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHERNVVVGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKEQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIHLEQVRR
                     TRGGKGE"
     gene            16982..17701
                     /gene="rsmG"
                     /locus_tag="KPH93_001078"
     CDS             16982..17701
                     /gene="rsmG"
                     /locus_tag="KPH93_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG1687912.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNVTFIHGRAEDFGKNENYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEIDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17864..18649
                     /gene="noc"
                     /locus_tag="KPH93_001079"
     CDS             17864..18649
                     /gene="noc"
                     /locus_tag="KPH93_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG1687913.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18785..19558
                     /locus_tag="KPH93_001080"
     CDS             18785..19558
                     /locus_tag="KPH93_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG1687914.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19560..20423
                     /locus_tag="KPH93_001081"
     CDS             19560..20423
                     /locus_tag="KPH93_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG1687915.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20456..21598
                     /locus_tag="KPH93_001082"
     CDS             20456..21598
                     /locus_tag="KPH93_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG1687916.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKMGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(22024..22923)
                     /locus_tag="KPH93_001083"
     CDS             complement(22024..22923)
                     /locus_tag="KPH93_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1687917.1"
                     /translation="MSIYGYTIFETVLEQNSFIKAASILNLTPSAVSHAIAKLERDVG
                     FQLFIRNRNGISLTREAEKILPHIRILLHSNEILEQEIDRILRSESGIIRIGTFNSIT
                     INWIPEIMKSFRQKYPNIEIRILQGNYINILEWIETSIVDLAFISNTVLPPHIESIPL
                     HKDRIICVFPKGFVPCNPGYVTIEDIQNMSFVMPKKDYDQEAEKILREYHLNVYSQFH
                     IDTDSAIVAMVEAGFGVAILSELIYKSCVGNVDTYPFVPEHYRVISLAYPNSRYDSFA
                     VQKMKEHIVNYTKEKGFFNFKSF"
     gene            23193..24389
                     /locus_tag="KPH93_001084"
                     /pseudo
     CDS             23193..24389
                     /locus_tag="KPH93_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            24458..25204
                     /locus_tag="KPH93_001085"
     CDS             24458..25204
                     /locus_tag="KPH93_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG1687918.1"
                     /translation="MTNKVPNLWNQLESLKKSYKWVDLSREVSPETPHWTGFPAMEVE
                     PFYLLDRGDGFNVNKYTLVTQYGTHVDAPIHFVADRKYLDEYTPMDMVLPLCVIDKSD
                     EVAKNSDYVVTVEDIQAWEKEYGNIPEGAFVAFRSDWSKRPDDSLDNYDSDGNKHFPG
                     WSIEALQYMVEKRNIAAIGHETCDTDAPILSNISGYQAETYILGENRYQVEFLVNLSE
                     LPAIGAIIFVTFPRIHKASGFTARCFAICP"
     gene            25237..25740
                     /locus_tag="KPH93_001086"
     CDS             25237..25740
                     /locus_tag="KPH93_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG1687919.1"
                     /translation="MSKKAMFVWCVGLGLLDGIYCILCSYLPHIQNYMWIGFISLPIY
                     FCGGAKLEEMPKYFCCAASGIFWGGLTLVVLDIGFISNIHLNMLVFVTIVVCIACFVH
                     LGVLTENVWKGLFSSCPMMFGGFAAIFSQGLAEWFWVLVTLTLGLIFGCIMASISTPI
                     TKWIDKN"
     gene            25988..26815
                     /gene="panB"
                     /locus_tag="KPH93_001087"
     CDS             25988..26815
                     /gene="panB"
                     /locus_tag="KPH93_001087"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG1687920.1"
                     /translation="MKNTIQTFKNAKSEGKKLSMLTAYDYSMAKIMDECNINGLLIGD
                     SLGMVVKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LVKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGITSEA
                     AKQLIEDAKLIEKAGAFSIVLEGVPAKISEIITNSISIPTIGIGAGINCDGQILIYQD
                     MLGIFEDFVPKFVKQYANVGDIMKDSIKNYILEVETGAFPQEKHSFSIKESELEKLY"
BASE COUNT         9706 a         3801 c         4048 g         9355 t
ORIGIN      
        1 catctttaaa acagtaggtc aatacttttt ttatattttt ttataaattt ttttcatatt
       61 cttttttcag cttctctact acctttttct ctagtctaga aacagtcatc tgtgatatcc
      121 ctagttcttt agctatacta gattgtgttt tatcaaagaa aaatctatct ttaaaaattt
      181 taacttccaa ttcatttaaa gtttcaataa acttattaat aaaatcttca tactctatac
      241 ttccaaagtt accttcttct tttccaatct tatcaataag agttatatct ttatcctctg
      301 aatcatcatt acttgatgaa tccaaagaca ttggttgata tccataagaa gcctccatgg
      361 cctctaaaac atcttcttca ctaacaccta tataatcagc tatatctttt acctttggat
      421 gcttcttatt ttcttgttct aatttaattt tcgcatcact tatctttttg cttaattcct
      481 gtattcttct tgggactcta agtgtccata ccttatctct aaaatacttt tttatttctc
      541 ctactatagt tggtgtcgca aagctggaga actcaaatcc tttctcaaca tcatatctat
      601 caatagcata aattaatgcc aatgaagcaa cttgatatat atcttcaaaa tcaacacctt
      661 tgtttatata cttcttagct aacaatcttg ctaaatacag atgtctttca ataagaatat
      721 ttctaatgtc tacattttta tttttattat ataaattaaa tagctctttt gtatccatat
      781 ttaggtaatg tgtagcatta gctacatttt tcattaaata tcaactccta aatatttagt
      841 catttttatt tttgtccctt gattatgttc tgattcaata tccacatcat ccattaaagt
      901 tttaattata aacaatccaa gaccactctc ttttggattc tctaaatctg gtgtacttat
      961 tgagctaaca tcatatcctt ttccattatc ttgaatttct atattaattg cattatctaa
     1021 aatattaaat actatattaa acacatcatc tttactgtgt tttattgcgt ttgtacatgc
     1081 ttcagatact gctactttta tatcttctat atcatctatt ggaaaaccca tcttattggc
     1141 aattccagat gctgttaatc ttattatact aacataatct ggatttgtag taatttccat
     1201 ctttatagtc tcacaagcca aagtttatcc ctccacttta aatattttat ctagacctgt
     1261 tatagtaaaa atctttctta cattactctt aggatttact atgtatatct ctttttcatt
     1321 tatttttaat ttttttaata cacctatcat agctcctaaa cctgttgagt ctatataatc
     1381 taaatcttta agattaatct ttacatctaa catatttttt tctattaaag catgtaaatg
     1441 ttcctttagt ttatctgctg tagaaacatc aagttctcca tcaagacaaa cattccaaaa
     1501 tttattttgt gaatctaaat ttgaatcaat attcatagac atataaaaac ctcctatttc
     1561 cttttaataa ttttaaatat atttcctata ctcaatattt tattaattac ataaactata
     1621 tcatctattc cctttgatat tgatgatgca ttttcaacaa tatcttgtat atgtctttca
     1681 ttataatcta caatcttata tgccttttca acaaccttat ttatgctgtt tagagtttta
     1741 gctaaataaa ttgctactat aagagcagag cttcctacta acactgctcc aatttgccac
     1801 ccccatgcat tcattagtta acatctccct catcatcaaa gcttttactt attactatat
     1861 cttcttctga aatttttata tcttcttcat tattatcatc gtctactaaa tttattattc
     1921 tctctttaac atcattaaat gtttcatgta gaacttcttt tggattttct ttgacatcat
     1981 taaacttctc ttttgtttct tttcttagtt cagaaccttt ttttggtgca aaaaacatac
     2041 caaatataaa ccctaaaata gctcctaata ataaaaatcc acctttttta caacttcttt
     2101 tattgcacat aacaattact cctttcaatt ttaaattttt atctaactac attatacaaa
     2161 aagaatagga tatttatcct attcttcttc aatatttatt ttggcaatcg aaactacatt
     2221 gacttcatca ttagtcttca ttaatttaac accacttgta actcttccaa acagtgatat
     2281 ttcattgact cttattctaa taagaactcc gtcagaattt ataatcatta tctcatcatc
     2341 ttcgttgacc atatctgcac ctacaattgt accagtcttt tcagatatat tataagtttt
     2401 aattccttta ccagctctta tttgacttct atactcttct atatttgttc tctttccaaa
     2461 accattctta cttacaacta acacatctgt acctttactg caaagattca tagacacaac
     2521 aaaatcttct ttacttaaag ttataccttt tacacccatt gctgttctac ccatatatct
     2581 tatatcattt tcatcaaatc ttatagacat accttcttta gttactaaaa gtacttcttg
     2641 ttttccgtct gtaagttcta ctccaataag ctcatcatca tctcttaagc ctatagcaat
     2701 aagaccagat ttatttatat ttttgaactc ttctctctta gtctttttaa caataccctt
     2761 tttcgttgca agtaataaat attcattttc atcattacca tcaataggta ttagagtagc
     2821 aattttctca tctgctgaca attgaagtaa attcactata gcagtccctt tagcctgtct
     2881 cttaccttct ggtatttcat atgcatttaa tttaaatact ctacctttat ttgtaaagaa
     2941 taatagtcta ctatgagtag ttgtagttac caagtgtctt acaaaatctt cttctctagt
     3001 tgtaagtgct gaaatacctc ttccacctct tttttgactc ttataagtat cagatggaag
     3061 cctttttata tatccaaagt gagtaagagt tattgctatt tcttcatcac ttataagatc
     3121 tctcatatct atttcgcctt cagcatgtct tatttctgtt cttctctcat cagagtaatt
     3181 ttcttttatt attgtaattt catcctttat tacatttaaa agtaatcttt catcagctaa
     3241 aatttctttt aatctattta tcttcttgat taaatcttca tattcagctt ctatcttatc
     3301 tctttctaaa cctgtaagtc tttgaagtct catatctaat atagcttgtg cttgtatttc
     3361 agttaatttg aatttttcta ttaaacctag cttagcttct tgcccagtct ttgaagctct
     3421 tatcaagctt ataacagcat ctatattatc taaagcaatc tttaatcctt ctaaaatatg
     3481 tgctcttgct tcagctttat ttagttcaaa tttagttctt cttgtaacaa catcttcttg
     3541 atgtttaata taatgatata atatttgttt aagatttaaa actcttggct gaccatctac
     3601 aagtgcaagc attattatgc taaaagtatc ttccatttga gaatgtttat acaaattatt
     3661 taatactata ttagcattag catccctctt taattctata acaattctca taccatttct
     3721 attactttcg tctcttaggt ctgatatacc ttctattctc ttttctttaa ctaactctgc
     3781 tattctctca accagtttag ccttatttac ttgataaggt atttctgtaa ctattatttg
     3841 ctgttttcct tttggtagtt cttctataaa agctctagac ctaactttaa cttttcctct
     3901 tccagttctg tatgcttctg ctatactttc ttttcccatt ataattgcag ctgtagggaa
     3961 atctggtcct tgaacaaatt ttattaaatc atctacacta cactctggat tatctatcaa
     4021 atatacagtt gcatcaatta cttctgctaa attatgtgga ggtattgaag ttgccatacc
     4081 aacagctata ccatttgagc catttactaa taaattagga tatctagctg gcaatactga
     4141 aggctctttt aatgactcat caaagtttgg tttaaagtct acagtttcct tttcaatatc
     4201 tctcagtagt tctaatgata atttactcat tttagcttct gtataacgca tagcagctgg
     4261 tgaatcccca tcaacagaac caaagttacc atgaccatct actaaaagtg ctctggttga
     4321 aaaatcttgt gccattctta ccatagcata ataaacagca atatctccat gaggatggta
     4381 cttacctaaa acgtccccaa caatacgagc tgacttcctg tatggtttat ctggagttaa
     4441 atttaactca ctcattgaat ataatattct tctatgaact ggctttaaac catctctaac
     4501 atcaggaaga gcacgtccag ctataacact catcgaataa tcaatatacg attttttcat
     4561 ttcttccgct atttcaatag ggagtatttt gttattttct tccatgtata ctaatcccct
     4621 ctctctatat atctaagttt ctaacaaatc ttgcattttc ttctataaat tcttttcttg
     4681 gagcaacctt atcacccata agcattgaaa atacttcatc tgcaattgca gcatcctcca
     4741 cagtaacttg taataaagtt cttgtctcag gattcatagt tgtttcccat aattgctctg
     4801 cattcatctc tccaagacct ttgtatcttt gaagttcaac accatttctt ccaatttcat
     4861 ctaataagat atttaactct ttatctgagt aaacataatg ttccttcttt tgctttgtca
     4921 ctttatataa aggtggctgt gctgcataaa catatccttc atctataaga ggtctcatat
     4981 atctaaagaa gaatgttaat aataaagttc ttatatgtgc tccatctaca tcggcatcgg
     5041 tcataattat aattttatga tacctagcct tatctatatc aaaatcttct ccaataccac
     5101 aaccataagc tgttatcata ttttttattt catctgaaga taatatccta tctagtcttg
     5161 atttctcaac attaagtatt ttacctctta atggaagtat agcttgacta tttctatctc
     5221 taccttgttt agctgaacct cccgctgaat ccccttcgac taagaatatt tcacttttag
     5281 atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa agatgtactt tccaatacac
     5341 tttttcttct tgttaattct cttgcttttt ttgcagcttc tctagctctt tgtgctctta
     5401 aagctttatc aactattatt cttgctgttg ctgggttttc ttctaggaaa gaccccagtt
     5461 catcaactgt tacactatca actatacctc tcataaagct attacctagc tttgttttag
     5521 tttgaccttc aaattgaggt tctggtaatt taactgacac tactgctgta agaccttctc
     5581 ttatgtcttc accaagtagg ttaacatcat tttcctttaa aaatttattt ctcttagcat
     5641 aatcatttac agttttagta agagctgctt taaatccact taaatgagaa cctccttcgt
     5701 gcgtatttat attgtttgca aaagaatata tgttctctgt ataaccatct gtatattgca
     5761 tagctagttc aacaaaacaa tcatcaacct ttttatctat ataaactatg tcatcatgta
     5821 taccagttct agatttattt aaatacttaa tatattctac caaaccacct gtataatgaa
     5881 attcttttct tttttcttgt ccttctcttt tatcttcaaa tactattttt attcctttat
     5941 tcaagaaaga aagttctcta agcctatact ccaatgtttc atatttaaac tctatttcat
     6001 caaatattgt ctcatctggc ataaattgaa tcattgttcc tttatcttga gattctccaa
     6061 ctacttcaag cttagacgtt ggtaatcctt tctcatatgt ttgcttatat atctttccgt
     6121 ttaagtaaac ctctgctacc atccattttg acaatgcatt aactacagaa acaccaactc
     6181 catgaagacc tccagatact ttatatcctc cgcctccaaa ctttcctcct gcatgaagat
     6241 tagttaatac tgtttctaaa gttgatttac cagttttagg atgtatttca acaggtatac
     6301 ctcttccgtt atcttttact aaaacactgc catcttcatt aatagataca tatatgtctg
     6361 aacaatatcc ttgtaaagcc tcatctatac tattatctac aacttcataa actaagtgat
     6421 gtaaacctct agggcttgta ctaccaatat acatacctgg tctttttcta acggcttcta
     6481 atccctctaa tacttgtatc tgacttgcac cgtattcttg tttcatttaa tttcctccat
     6541 tctctatgct aataacatcg ccattttcaa tattaaatat tgtaacattt ttttcatcaa
     6601 atattttttt atgtgaaacc tcagcacttg ttataaacat ttgaacattg cttaaattat
     6661 ttactaaaag tttttgtctt gcttcatcta actcactaaa aacatcatct aaaattaata
     6721 ctggatattc ttcaacttca ttttttatta attctatctc tgataacttt aatgatattg
     6781 aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc atctaaatca tttatgaata
     6841 tatttaagtc atctttgtgt ataccatatc tggtagtctt agattctata tcattaggtc
     6901 tattcgatga caacttggct aaaaatttat tatatactgt atctatagta tcttcatcag
     6961 ttatatttat ttgattttta tatcgaattg ataatctttc aactccatta gtcaaattca
     7021 tatgcatatt ttcagatata ttagctatct tttttataaa gtctctacgc aaaatataaa
     7081 tataacttcc atactttgct aacgtatcat catatacatc taataaagct tcatcaacat
     7141 gtatattttt aagcacccta ctcctttgag aaagtgtttt attataattg gtaagatatt
     7201 tataatattt aggtataatc tgactaatct ctttgtctat aaatgccctt ctttctttag
     7261 gaccctcttt aaccaatctt aaatcctcag gagaaaatat aacaacattt aagttgccaa
     7321 gtagttcttg tatcttttgt aaaggaactt tatttattct tatccccttt ttatcttttc
     7381 caataattag ctctattaga ctatatttat tatattttga aaaacttcct ccaatatata
     7441 agttttcact attaaatcta accatttctt tatctttgtt agtcctaaat gattttccaa
     7501 aagaaagcat ataaatagat tctactatat ttgtctttcc ttgaccattt ttaccaacaa
     7561 gtagatttac tttcccatta aattccagat gcaacttttt ataatttcta aaattaacaa
     7621 gttgcaaact tttaagtttc acaaaaagtt ctcctttttt tacaatactt ttattttagt
     7681 tccttctatt tcaactatat ctcctgattt tatctttttt cctcttctta attctatttc
     7741 atcatttact gtcactaaac cttcttgaat taaaaatttt gcatgacctc ctgttgatgc
     7801 aatctctgct aatttaagaa attgatccaa ctttatatat tctgattcta tagttatttc
     7861 agtcataata gttcctcctt gtcactattt taaacaaatt cctattgata cactaaaagt
     7921 atattatacc ataaatatgc ttataaaagg agttgtctaa gattagaaaa attttctaca
     7981 aaagacaact ccttcatgtc tgctatatat ttgatgatat tcttactgga agcagtaagt
     8041 aaatataatt aacatcatct gttggtttaa ttatacaagg atttacattt gtagtaaatt
     8101 ctataaatat ttcctcatta tctatatttt tcaaaccttc aataaaatat ctagaattga
     8161 aagctatatc tagataatct ccatctaaat ctatttcaac ttcttcatac acatttcctt
     8221 tttctgtatt agaagtaatt gccattactt tatctcttat agataacttt ataagattat
     8281 ttttttctga ttgagacaat aaagacgctc tctctatact atttaaaagt tccttagtgt
     8341 ttaatttaac tctgctgtta tgttctcttg gcaataattt tttataatca ataaaatctc
     8401 cttcaagcaa tcttgtaata attttcgtat cattaattat aaaaatagca tttttttcgt
     8461 taaatccaac tttaacatta tcctctccag aaagcaaact atttacatct ataagtgtct
     8521 ttccaggaat tatgactttt atattttcag ttaaactatc aactgaacaa ctttttacag
     8581 ctaatctata tccatcaatt gctactaaat ttaagtttct atctacaatt tctaaaagtt
     8641 ctcccattaa aacaggtttt gtttggtctt gtgagatagc aaatacagtt tgtttaatca
     8701 tattttttag gatttcttga ggtatgctat ataaatcttc ttcatttaat tctggtaatt
     8761 taggaaattc ttttgctgca taacctttaa ttttaaatct tgaatttaca caatttatgt
     8821 aaatattatt ttctgaatca gtttctatct caacgaagga atctggtaat tttctaatta
     8881 tatctccaaa tagccttgca tctacaacaa catcaccttt ttctatgatt tctgcttgta
     8941 cataagtttc tattcctatt tcagcatcat atccagtaag ttttaattga ccttcttctg
     9001 tagatataag tatacctttt aataattcta ttgttgtttt tccattaata gctttttgag
     9061 caattccaat tctatttgct aggatttttt gattacaaat tattttcaat gtggataacc
     9121 tcctctggct ttttatatat aaaaataagt taaaatcata gtagtaatag tagtaggtgt
     9181 tgttgataat gttaataagt atgtataaca ttataaatac aaagatatta acatgtgtat
     9241 aagatgttaa tatctttgtt attttagact tgatagtttg ttaataattt ttgttcaaaa
     9301 aaagtattaa caggttattc aagagatata aacatacaat tgtgtataaa ttatcccttc
     9361 aaatctgata taattttgtc tattttagtc ttaatttctt cactttcttc tatatcctta
     9421 gatactttgt catgagcatg aattactgtt gtatggtctc tacccccaaa ctcttcacct
     9481 attttaggaa gggataaatc agtaagttcg cgcgttaaat acatagctat ctgtcttgga
     9541 taagctattg aacgtgttct ttttttagaa ttaaaatctt ccatctttaa attgaataca
     9601 gaagatactt tttcttttat tctgagaaca tttatttctt catttttaga agttgttata
     9661 atatctttta aagcttctgt agcaagatca aaagatatta ccctattgct aagagaagaa
     9721 taggcaacca ctctagttaa tgcaccttct agttccctta tgtttgactt aatatttttt
     9781 gctatatatg acataacctc atttggcaca tctatacgtt ctagttgagc cttttttcta
     9841 agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat cagttataag tcccatttca
     9901 aatctcgatc ttagcctatc ttctagtgta ggtatgtctt ttggcggtct atcactagat
     9961 ataattattt gtttgtttgc ttcatgaaga gtattgaatg tatggaaaaa ctcttcttga
    10021 gttctttctt ttcctgctat aaattgaata tcgtcgatta ataatacatc tacgtttcta
    10081 tatttattcc taaattcttc gtttttatcg tcttttatag agtttataag ttcattagta
    10141 aatttttctg aagaaacgta aactactttt gagtcttttt tttgactaac aatatgatga
    10201 cctatagcat gcattaagtg agtcttacct aatccaactc ctccatacaa aaaaagtggg
    10261 ttgtaagctt tagctggaga ttctgcaact gcaacgcatg cagcatgtgc aaatctattg
    10321 ctattaccaa taacgaatgt atcaaatgtg tattttggat ttagattggg gtaaagaact
    10381 ctataattta aatctgtgct atcagaattt aaatcagcaa cttctttttc gctcaataca
    10441 aatttaatat tatatttttt taatgacagc tgactaatag catcttcaat taaatgtaaa
    10501 tatctattct ctaagatatc tttattaaaa tcactaggag ccagtaatat taaatcatta
    10561 agatgtattt ttaatggtac gatgttttta aaaaaggtat taaaacttac tgaagttaag
    10621 tcacctttta ttaattgtag ggttttgtcc cataaagaaa ctatatccat aatataaacc
    10681 tccgaacaga ataattaata ttttccacaa agttattaac agaaaagcaa aaatatatat
    10741 aaattttaca gaataactaa atttatggat aatggtgttg ataaattaat aatagtataa
    10801 attattaaaa aaatatgaaa agatataaac aatatgtaga taaagttata aataacataa
    10861 acatacaata aatggtgatt ataactagag tattgtcagt aatcttaaaa gttatacaca
    10921 aaagtatcca caagctgtga ataatttcta ataagttgat aacatttatg cacattatca
    10981 acaagtatat ttataatatc aaaatatatg tcaaacttca atgatttgta actaatttat
    11041 ccacaatatt aactaattgt tgataaaaat ataaacatgt taaaattact ttatttgtgt
    11101 atataattga aaaaaataat ttagaataaa attaaataga tttttactag tattttcaat
    11161 aaaaaaaata cataatatat atatggtaga ttttgtgata taagcgtccc aaaaactcgt
    11221 tgacaacagt agtaaaagta tttataatta tatgtgttat gtttactttg agggtaaagt
    11281 tattattttt ataaacggaa aataaaatct gaaaggcggt gcaaaataat gagtaaaaga
    11341 acttatcaac caaaaaagag acaaagaagt aaagaacatg gtttcagaaa aagaatgaag
    11401 acgtctaacg gaagaaacgt gttaaagaga agaagagcta aaggaagaaa tagattaact
    11461 cattaattac aaaagggccg ctgcaaaggt ggcctttttg tgtaaataat tacaattcat
    11521 agctgtgttt atggaggtta actatggact ttaataggac taaagggttg aaaaaagact
    11581 ctgattttag aaaagtatat aaacacggca aatcttttgc aaacaaatat ttagtaatat
    11641 atatacttaa aaataaatca gattatagta gagtaggtat ttctgtttca aaaaaagtag
    11701 gaaaagctat tactaggaat agagtaagaa gattaataaa agaagcctac agattaaata
    11761 ttgatgaaaa aattaagcct ggatatgata tagtatttat agcaagggta tctagtaagg
    11821 atgctacttt taaagacata gataagtcta taaaaaattt agttaaaaga acggatattt
    11881 ctatttaatt gtatgctata aatatggagg ttaatgactt tgaataaaat aatttatata
    11941 ttaaaggaaa taagtaagta tttatcgaat ttatgtattt atttagtgag attttatcaa
    12001 aagtatatat ctcccttaaa gggacctacc tgtagatttt atccaacttg ctcacaatat
    12061 tcaatagagg cgtttaagaa atatggattt gtaaaaggta tgtatttaac gataagaaga
    12121 cttttaaaat gtcatccttt tcatccagga ggttacgacc ctctaaaata aaagagagta
    12181 actattagga ggtataaaaa aattgaatat aataggaaat ttcttaggat tgattcttaa
    12241 ggtaatattt gaatttgtta atcattatgg tatttctata atacttttta cgatacttgt
    12301 aaagattatt ttattaccat taacaattaa gcagactaag tcaacaaagg caatgcaaga
    12361 tatacaacct cgtataaaag agatacaaga aaaatataaa aataagccag aaaaacaaaa
    12421 tgaagaaatt gttaagttat atggagaagc taaaattaat ccactatctg gttgtttacc
    12481 tttattaata cagtttccta tattaatagg attatttagc gttttgagag aaccagttgc
    12541 tcatggcgtt tttgcaaaca aagctgcatt tttagcagct gacaatgggt ttttatggat
    12601 aaaaagctta acatcaccag attatgtatt agcagtattt tcaggagcaa gtgcatatgt
    12661 catgcaaaaa gtaatgacac caaaagatca gttacaaggc tctatgaaag taatgacgta
    12721 tgtaatggca ggtatgtctt tctattgggg atttatattc ccagcaggac ttacattgta
    12781 ctggacagta agtaacttat tctcaatagc tcaatattac ttaataatga atcctttaaa
    12841 agctaaactg gctgctaact ctaaggagga aaaagttaat gagaaaaagc ctaaaattaa
    12901 taaaaagtaa gagtaaagag gaagctataa gtaaagctat agcagagttg aatttaaaag
    12961 aagaggatat agaagtagaa atacttgaaa atccaagtaa agggttttta ggttttatag
    13021 gtgctaaaga tggtacttat gaaatttttg ttatcgagag ggaagtagat gtagctaaaa
    13081 attttataga ggtaatgttg aaaaatgcaa atgttgatgc caaagtcaat gtatctcaaa
    13141 aagataattt aatcatggtt gatattgaag gcaaagaagc tgcaagttta attggtagaa
    13201 gaggggaaac tttagattct attcaatttt tgactggatt agctcttaat aaaataaata
    13261 aagattctca tacaagagta cttgtagata tagaaaatta tagatcaaaa cgagaagaat
    13321 ctttaataag atatgctaat aaagttgcta gagaagtagc gaaaacaaga aaaactaaaa
    13381 aattagatta tatgaatcca tatgagagaa gaataataca ttcagctctt caaaatgaca
    13441 aatatgtaat tacatatagt gaaggaaccg atccatatag aagattagta attgagtgta
    13501 aaagataaag tcaacctcct aaacattagg gggttttatt ttttaatatt attaaatcat
    13561 taatgattgg ttaaatgtaa atctatatgt aaaaaattat gaattaagag gtgataaatt
    13621 tgtttataga tgatacaatt gcagcaatag ctacagcacc tggagaaggt ggtataggta
    13681 tacttagaat aagtggagaa agagctttga aggttgcaga agaaatcttt aaatctatgt
    13741 cgggaaaaag tattgaagaa tacaataaga gaacattaat atatggaaat atagtggata
    13801 atgaaaatat aattgatgaa gttttattgg catatatgaa aggaccaaac tcctatacag
    13861 gagaagatgt tatagaaatt aattgccatg gtggatttat ttcggtaaag aagatattag
    13921 aattaatact atcaaaagat gttagattag cagaggctgg tgagtttact aaaagagcat
    13981 tcttaaatgg aagaatagat ttatctcaag cagaggctgt aattgatgtt ataaaagcca
    14041 aaactgatat agctcatgaa gttgctcaaa atcaattaga gggctcttta tcaaagaaga
    14101 taagagaatt gagagataaa gtcactgaga tattagctca tgtagaagtt gctatagatt
    14161 atcctgagga agatattgag catataacat atcaaactct taaagaaaag actgatgaac
    14221 ttaaaaaaga aattaaaaaa ttatatgata cagctgaaag tggaaaaata cttagagaag
    14281 gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa atcttcactg cttaattcta
    14341 ttttaggaga gaatagagca atagttactg atataccagg aactactaga gatgttatag
    14401 aagagtttgt aaacataaaa ggtatacctt taaaaattgt tgacacagca ggtataagag
    14461 atactgatga tattgttgaa aaaataggtg ttgaaaagtc taaagaatca tttactagtg
    14521 cagatttgat agtaatggtt ttagatgcat ctagaaagtt gtcagaagaa gatatagaga
    14581 tattagaaaa acttaaggat aaacaaacaa tagtgttgtt aaataagaat gatttgaaac
    14641 aggagattga agaagaaaaa atacttaaat atgtagaaaa taatagtata ataaaaatat
    14701 cagctttaca acaagaagga atagaagaat tacaagataa aatagagtct atggtatata
    14761 aaggtagtat caaaaataat tctagtttag tagtaacaaa ttcaagacat aaagatgcac
    14821 ttagtaaagc atataaatct gcaacagatg ctttaattgc cttggaacaa agcatgccat
    14881 ttgactttgt agaagtcgat ttaaaaaata tatgggacta tcttggatat attaatggtg
    14941 atacagtaac agaggattta ctagataata tattccataa tttctgtatt ggtaagtaat
    15001 gtacaaaagt aaatttgttt ttattataga gatttttaaa aaatttgcag tgtttctcat
    15061 gaaacattaa ttttgaaaag aaaggaatta taaatgataa agtttgaagc aggaaaatat
    15121 gatgttatag tagtcggagc aggacatgct ggatgtgaag ctgctttggc tactgcaaga
    15181 atgggatata aaactttgat aataacaatg tctttggatt ccatagcatt aatgccttgt
    15241 aatccatcaa ttggagggac tggtaaaggt caattagtta aagaaataga tgccttaggt
    15301 ggtcagatgg gactaaatat agacaagaca tatatacaaa gtagaatgtt aaatactgcc
    15361 aaaggtccag cagtacactc tttaagagca caagcagata aatttaaata tcatgaagaa
    15421 atgaaaaaga cattagaaga tgaaccaaat cttgatatag ctatggatga agttgtagaa
    15481 atacttcatg aaagaaatgt tgtggtagga gttggaacta aactaggatg ttcatttaaa
    15541 tctaaagctg taatattggc cactggagtt tatctgaact caaagatata tatgggtgaa
    15601 gttgcatttt atgaaggtcc taatgcattg gggtatgcaa aatatcttac agatagctta
    15661 gttgaacttg gtcttagaat gagaagattt aagacgggaa ctccagcaag agttcataga
    15721 gatagtatag acttctctgt aatgtcttta caagagggag atgaaaaagt aactccattc
    15781 tcatttatga atgaaaatat agaaaaagaa caagagcctt gttatttaac aagaactaca
    15841 gaagaaactc aaaaagttat actggataat ttaaaaaggt ctgctatgta tagtggagta
    15901 atagaaagta ctggtccaag atactgtcct tctatagaag ataaagtagt acgatttagt
    15961 gataaaacat cacatcaatt atttatagaa ccagaagggt taaatactaa agaaatgtat
    16021 atacaaggaa tttcaactag tttacctttt gaagttcaat tagatatgta taaaactata
    16081 aaaggacttg aaaattgtaa gataatgaga cctgcatatg ctattgaata tgattgtgtt
    16141 gacccaactc aactaaaaat aagtttggaa ataaagggag ttgagaactt atttagtgca
    16201 gggcaattta atggaacttc tggatatgag gaagcagcag cccaaggtct tatggctggt
    16261 ataaatgcag ttagaaaaat agaaggaaaa gaaccatttg tattagaccg ttcagaagct
    16321 tatataggtg tcttattaga tgaccttgtc actaaaggaa ctaacgagcc ttacagaatg
    16381 atgacatcaa gagcagaata tcgactatat ttaagacaag ataatgcaga tatgagatta
    16441 actcaaaaag gttatgatat aggattagtt aaaaaagata gatatgaaag gttcctaaat
    16501 aaaaaggctg ctgtagagaa agaatttgaa agattaaaaa atgaaagagt tacaccaaaa
    16561 gaagtaaata gtttgttaga agaaaaagga gcaacaccaa taaaggttgg aatatctcta
    16621 tatgaatttt taaagagacc agaggttact tacgaattgc ttgaagaatt agggaaaggc
    16681 gctggagaag atgtttcaag agaagttaaa gaacagtgtg taataataac taaatatgaa
    16741 ggttatatag agaaacaatt gaaacaaata gaccaattta aaaaacttga gaataaaaag
    16801 ctggatgaga aaataaatta ttcatctata gaaggtctta gattagaggc tagacagaaa
    16861 ttagatgata taaaacctat ttcaataggg caagcatctc gtatatctgg tgtttcccca
    16921 gctgatatat cagttttact tatacaccta gaacaagtca gaagaactag aggtggcaaa
    16981 ggtgaataat atagaacttc tgaaaaacgg gattgaaggt tttaatatag atacgaatga
    17041 cagtatgtta gaaaaattta aaatttatag agagatatta gttgactgga ataaaaagat
    17101 gaatcttact ggaatagagg atgaaaaaga agtatatata aaacattttt tagactctat
    17161 ttcagctgta aagaatggat atataaaaaa tgggatgtct ataatagatg ttggtacagg
    17221 cgctggtttt cctggaatac cattgaaaat atgtctagat agcttagaac ttactttgtt
    17281 agattcatta aataaaagaa taaacttttt agaggaagtt agtagagtac tagagttaga
    17341 taatgttaca tttatacatg gaagagctga agattttggt aaaaatgaaa actatagaga
    17401 aaaatatgat gttgctacag caagagctgt tgctggatta ccgattctta tggaattttg
    17461 tgtaccattt gtaaaagttg gagggtattt tatttgctta aaaggcccga atgctaattt
    17521 agagttagaa gaatcacaaa aggctattga tgttttagga ttggagtata ttgaaaaaat
    17581 agatgtagag ttaccagaga tagatttaaa tcataatata ttagttttta aaaaagtgat
    17641 ggaaacacca gttaagtacc ctagaaaagc tggtaaacca gccaaaaatc ctattaaata
    17701 atgaaaaaat tattattggt aagcttagtt atttctcagg aaataattaa gcttaccgta
    17761 aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa ataaatttgt atgtacatat
    17821 agagaacaaa aataatttat atctaaaata agagaggtat gttatggaag ataaaagagt
    17881 tatggagata cctatagaag atgtagttcc aaatccatat caaccaagaa aaatattttc
    17941 acaagtttca ttggaggaat tgagcaattc tataaaggta tatggtataa ttcaacctat
    18001 aacagttaga gcaaaagatg gcaaatatga actaattgca ggagaaagaa gattaagagc
    18061 agctaaatta gcagagctta aaaccatacc agcaattata aataatatga acgatgaatc
    18121 ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa gacttaaatt tcattgaaga
    18181 agcaattggt tatgaaaact taataaaaga acatgctttt actcaacagc aattagcaga
    18241 aaaattgggc aaaaatcaat ctactgtagc aaataagctt agaatattaa agcttccaaa
    18301 cgatataaaa atgaagctaa tagagaataa tttaacagaa agacatgcta gagcatttct
    18361 taaactacca agtgaagatt taatgcaaag tgtgttagac aaagtcataa aaaatgaatt
    18421 gactgttaaa aaaacagaaa aattgataca agatgtatta gaagaaacta aagttcaaga
    18481 agagcctgat aaaaaacaga atataaaggg tgctatgagt ataagaattt acataaatac
    18541 tataaagcag gcctttgatg ctatatcaaa tacaggaatt gatgctaagt ataatgaaat
    18601 agataaggga gattatatgg aggttgttgt aaaaatccct aaaaaataga gattactgct
    18661 taccagtaat ctttttttgt aatataagtc ttgtaatata aattgattta tagacattat
    18721 ttatacttaa aaaacaccta ttcaacattt aaaattaatt taattcttaa agggggggaa
    18781 catcatgggc aaagttatag ctgtattcaa tcaaaagggt ggagtcggga aaactacaac
    18841 aaatgtaaat ttaagtgcta gcttaggaac tttgggaaag aaaatattag tattagattt
    18901 agacccacaa ggaaatacaa ctagtggata tggtattaat aaaaacgaag tagaaaatac
    18961 aatctatgaa attatgttag atgggttaca tattaaagaa gcaataataa gtactgaatt
    19021 tgagaatgta gatgtagttc catcagctac tgaactatca ggtgcagaga tagagcttac
    19081 atctaaaact aatagagagt atattttaaa aaattccata aaagcggtaa tagatgaata
    19141 tgattatata tttttagatt gtccaccctc actggggatg cttacaataa attgtttaac
    19201 tgctgtagat agtgttttaa ttccaataca atgtgaatat tatgctttag aaggtgttag
    19261 tcaattaatg gagactataa aattagtaaa atcaagatta aatgctgata tagaaattca
    19321 aggtgttgtt ttaagtatgt ttgatggaag agctaattta tcaatacaag tagttgaaga
    19381 agttaagaaa tactttaaag gtagtgtata tacaactcta attccaagaa atgtaagact
    19441 tgcagaagct ccaagtcatg gaaaacctgt aatttattat gataaaagat gtagagggtc
    19501 tgtagcttat ttggagctag cagaagaatt tatagattta gaagaggagg aatggtagta
    19561 tggagaataa gtctaaaaga agtaatagat tagggagagg tcttagtgca ttgataccag
    19621 aaattaaagg agaaacttca gaaaaagaaa tagtcaacat agatatagac aaaatatatc
    19681 caaacgaagt tcaacctaga aagcaatttg atgaagaaaa gataaaagta ttgtctgact
    19741 ctataaaaaa ttatggtgtc ttacagccaa tagtggttaa aatggatgaa aataataaat
    19801 atatgataat agctggagaa agaagattta gagcatcaaa acttgcaaat aaaaatcaaa
    19861 tacctgctat aattaaagat atagatatga aagatataat ggagatagcc ttaattgaaa
    19921 acttacagag ggaagattta aattcaatag aagaggctct agcatataag agtttaatag
    19981 agcattacaa cgtaactcag gaagaaatat cagaagctgt tggtaagagt agacctcata
    20041 taactaatac attaagactt ttaaatcttg gacaagatgt tatagaaatg atagacagtg
    20101 gtagaattac agcaggacat ggtaaggctt tattgagaat tgcagataaa gatttgcaac
    20161 ttcaaatagc gaaaaaaata gaagaagaag aactttctgt aagagaagtc gaaaatatag
    20221 ctaaaaaaat atctgaaaat aaacaagaag aacctaaaaa atctaaacct aaggatgtat
    20281 ttattttaga tgttgaagat aaacttagaa atatatttgg aacaaaggtg aatatttcta
    20341 aaggaaagaa aaaaggtaaa atagagatag aatactataa tgatgatgat ttaaatagta
    20401 ttgtatcaat gcttcttgaa tagaacaaaa atataaaaca gagaggaaga ttgttatgat
    20461 ttatttagat aatgctgcaa ctacatatcc aaaaccagaa agagtgtata atgctgtttt
    20521 agattgtatg aaaaattatt gtgcaaaccc aggtagagca ggccataaac ttgctatgag
    20581 agctgctagg gagatatatg atacaagaga gaatatagct aagttgttca atgtaagcaa
    20641 tcctatgaat atagtattta catctaatgc aacagattct ttaaatttag cgataaaagg
    20701 tgtattacaa gaaggagacc atgtaataac aacgtctatg gagcataatt cagtaataag
    20761 acctataaaa gcattggaaa aaatgggtat tgaaaatact gtagtaaaat gcgattatga
    20821 gggattttta gattatgaag atttagaaaa atcaataaag aataatacta aattgattgt
    20881 aacaacacat gcgtctaatg tatgtggaac tttaatagat ataaaaaaag ttggagaaat
    20941 agctaaaaaa cataatatac tatttttagt agatgcatca caaacagcag gtgtttatga
    21001 tatagacgta aatgaatgta atatagatat gttggcaatg ccaggtcata aatgcttatt
    21061 tggacctcaa ggaacaggta tattatatgt aagagaaggg ttgaacttaa atatattaaa
    21121 agaaggtgga acaggtagta aatcagaaga aatagttcaa ccagagttat tcccagataa
    21181 atatgaatca ggaactcaca atactccagg tatagcaggt cttaaccaag gtatactatt
    21241 tatatttgaa agaggaatca acaatataag acaacatgaa gaagaattat gtcaatatat
    21301 gatagacaaa ttagaggaag taccagatat aaagatatat ggaccaaagg atagtaaaaa
    21361 aagagcatca gtaatagctc taaatatagg agatatggat tctggagagg ttactttctt
    21421 attagatagt gattataata tagcaactag atcaggaata cattgttcac cactagcaca
    21481 taccacttta ggtactttaa aacaaggtgc agtaaggttt agtataggat atttcaacac
    21541 aaaagatgag atagataaag cagtggaagc tttgaaaaaa atttcaaaaa ataaatagca
    21601 tgagatttaa taatatagaa ataaaaaatg gggatgtttc aaaataaaaa tttgaataga
    21661 gtcttgaaat ttttatgtat attttctaaa taaaaacttc aagaccctat taattaaatt
    21721 actaattttg ctaattttca taaaatctat taaattactc ataaagttat atattttcaa
    21781 aggcttaagt cttgtatgaa attacttgtt ttaatttttt atataactat gacttttgca
    21841 atctttttag ttgaaaaagc accttcaatc atactaaaat aataagatta caatgctaaa
    21901 ttattttatc ctctattata actataaata caaactggtg ttcctcatct tatgaaaaag
    21961 gtattgctca gctttgataa aactgacaac taagtaataa aaatattata ctaattttac
    22021 tttttaaaaa gacttaaaat taaaaaaacc cttttctttt gtataattta caatatgttc
    22081 tttcattttt tgaactgcaa aagaatcata tcgtgagttg ggataagcta aactaattac
    22141 acggtaatgc tctggtacaa atgggtatgt atcaacattg ccaacacaac ttttataaat
    22201 tagttctgat aaaatagcaa ctccaaaccc tgcttctacc atggcaacaa tagcactatc
    22261 agtatctatg tgaaattgag agtacacatt aagatgatat tcacgaagaa ttttttccgc
    22321 ttcctggtca taatcttttt taggcataac aaaggacata ttttggatat cctctattgt
    22381 aacataccca ggattacaag gtacaaatcc ttttggaaag acacaaatga tacggtcttt
    22441 atgaagcgga atagattcaa tatgtggagg aagtactgtg ttggatataa aagctaaatc
    22501 cacaatagag gtttcaatcc attctaggat attaatatag ttcccttgaa gaatacgtat
    22561 ttcaatatta ggatatttct gccgaaatga tttcataatc tcagggatcc agtttattgt
    22621 aatactatta aaagttccaa tacgaataat accactttct gaacgtaaaa tacgatcaat
    22681 ttcttgttct aaaatttcgt tactatgtaa taaaatacga atatgaggta aaattttttc
    22741 agcttctctt gttaaactaa taccatttct atttctaata aacaattgaa atcctacatc
    22801 tctttctaat tttgcaatcg catgacttac agctgacgga gttaagttta aaatacttgc
    22861 tgcttttata aaactatttt gctctaaaac tgtttcaaaa atagtatatc cataaattga
    22921 catatgattt actcctttcc tttagatgag taaaaactca tctaaaacga taaaaagatt
    22981 cgctttattt tataataata atctactatt attaaattat aaatacaagt gatcatttgc
    23041 attttttact caagataata aaaatagtac caccaaaaat acaagaatta aaatattatt
    23101 tctgtttaaa aatgagcgga aacggaacct ttgctcatct aatctcatat aattacagac
    23161 aaatataaac ttgataaaaa ggaggaacta atatggtaaa atttgcaaaa agaatggaaa
    23221 atatgaaaaa ttcagcaaat attgttcgta atctttttaa ttccatgaca gatcctgaaa
    23281 ttatctcttt tggtggaggt gcaccagcag tagaggcttt accaattgat attataagag
    23341 aaatttctaa tgaagtttta gcaaaaaaca aaagagggat tgaagcctta cagtatagtc
    23401 acccatatgg tttaagagat ttgcgtgaag cagttgctca gaaattatta gaaccaaagg
    23461 gagtacatgc aagtccagag gacattatga ttgtaaatgg tggtttggaa acaatgaatc
    23521 tgatttgcca ggtattcatt gatccaggag atgttatttt agtagaatca ccaacatttg
    23581 tacattgtgt tgaaattttt gaaatgtttc aggcaaaatg catagcagta gatatggatg
    23641 aacatggaat cattccagaa gatatagaaa gaaagattca aaaacatcac ccaaaaatgg
    23701 tttatgtaat tcctactttt cataatccaa ctggtaggac attgactaca gagcgaagaa
    23761 aaaaattggc aggtattggt agtgagtatg atgttttgat tttagaagac gacccttatc
    23821 gtgatttgag atatagtgga gaggagttac ctcctattaa agcatttgat aaaacaggac
    23881 atactatttt agcaaacagt ttttctaaaa tcttttctcc aggagcaaga cttggttatg
    23941 ttttagctga acatgcaatc attgaacgat tacttgatgc aaaaacagca actaattctc
    24001 atacatcagc tatttctcaa gtattgtgtg cagagttttt caatcgtggt tactttgatg
    24061 aacacttgaa gaatattcga gatatttata gagaacgcag agatgtgatg attgaaagca
    24121 ttgataagta tttaccaaaa ggtactaaaa gggtatttcc agatggtggt ttatttactt
    24181 gagtagagct acctgaaaat atcaatacaa ctgaattatt gaaagaagcg gtagaacata
    24241 aagcagcttt tgttgcagga gaaggattct ttacagaagg tggtggaaaa ggacgaaatt
    24301 gtatgagaat tagctttggc aatgtacctc ctgaaaaaat tagaattggt atggaaaggc
    24361 ttggacaatt aatcgcttcc aaaatgtaaa taattactta tataaaatac aactttatat
    24421 tttaagattt ataatataac caaaggagga attacttatg acaaataaag taccaaactt
    24481 atggaatcaa ttggaaagct taaaaaaatc atataaatgg gtggatttga gtcgtgaagt
    24541 aagtcctgag acacctcatt ggacaggctt tcctgcaatg gaagtagaac cgttctactt
    24601 attggataga ggagatggat ttaatgtaaa taaatataca ctagtaactc aatatggaac
    24661 acatgtagat gcaccaattc attttgttgc tgatagaaag tatttggatg aatatacgcc
    24721 aatggatatg gtacttccac tttgtgttat tgataaaagt gatgaggttg ctaaaaattc
    24781 agattatgta gtaactgttg aagatattca agcatgggaa aaagaatatg gtaatattcc
    24841 agagggtgca tttgtggcat ttcgttcaga ttggtcaaaa agacctgatg attctttaga
    24901 taattatgat tcagatggta ataagcattt tccaggatgg agtatagaag ctctgcaata
    24961 tatggttgag aagagaaata ttgctgcaat tggacatgaa acttgtgata cagatgcacc
    25021 aattttatca aatataagtg gatatcaggc agaaacatat attttaggag aaaatcgata
    25081 tcaagttgaa tttttagtaa atttgtctga gttacctgcg ataggagcta ttatttttgt
    25141 aacattccca agaatacaca aagcatctgg ttttactgcc cgttgttttg caatttgccc
    25201 ataaggtttt aactgttaga agggaggggg agacagatga gtaaaaaagc aatgtttgtt
    25261 tggtgtgttg gcttaggtct actagatggc atctattgta ttctttgttc ctacttaccc
    25321 catatacaaa actatatgtg gattggtttt atttctcttc caatttactt ttgtggaggt
    25381 gcaaaattag aagagatgcc aaaatatttt tgttgtgcag caagtggtat attttggggt
    25441 gggctaacac tagttgtatt agacatagga tttatatcca atatacattt aaatatgctt
    25501 gtgttcgtaa caattgttgt atgcattgct tgctttgtac atctaggtgt attgactgaa
    25561 aatgtatgga aaggattgtt ctcaagttgc ccgatgatgt ttggtggatt tgcagcaatt
    25621 ttttctcaag gattagcgga gtggttttgg gtacttgtaa cacttacact agggttgatt
    25681 tttggttgta ttatggcctc aatttcaaca cctatcacaa aatggattga taagaattga
    25741 aataaaataa ttattcaata atttttaaaa agttaattta tttatgaaat ataatagtat
    25801 attatttcaa aatagtttaa aaggaaaagt attaaaaaac ttttcctttt aatttaaaac
    25861 ataaatacat ttgccacata agtgcttctt taaaatctag tattatcttt actagatata
    25921 ggagtaagtt gttttactaa attagtttta tatgaagata tttacaaaat tttaggagga
    25981 aaaaacaatg aaaaatacta tacaaacttt taagaatgct aaaagtgaag gaaaaaaact
    26041 atctatgctt actgcttacg attactcaat ggcaaaaata atggatgagt gtaatataaa
    26101 tggtctttta ataggagatt cattaggaat ggttgtaaaa ggtgaagaaa atactctctc
    26161 tgttactata gatgaaatta tatatcatac aaaagctgta aaaaatgggg caaaaaatgc
    26221 cttaatagta agtgatatgc cttttctatc ttaccatgtt tctatagaag atgctgttaa
    26281 aaatgctggt agacttgtta aagaaggagg agctcatgct gtaaaattag agggtggatc
    26341 aaatgttata aagcaaatag aaagcattgt aaatgctcaa atacctgtaa tgggtcattt
    26401 aggactgact cctcaatctg taaattcatt tggtggattt aaggttcaag ggattactag
    26461 tgaagcagca aaacaactta ttgaagatgc taaattaata gaaaaagcgg gggcattttc
    26521 tattgttctt gaaggtgttc ctgcgaaaat atctgagata ataactaact ctatttcaat
    26581 tcctacaata gggataggag caggaattaa ttgtgacgga caaattttaa tttaccaaga
    26641 tatgttaggt atatttgaag atttcgtacc taagtttgtg aagcaatatg caaatgtagg
    26701 agatataatg aaagattcaa ttaaaaatta catactagaa gttgaaacag gggcatttcc
    26761 acaagaaaaa catagttttt caataaaaga atctgaatta gaaaaattat actaagatta
    26821 aggatgttat aagctgtcat atataacaat ttattattat atttagagat tgattatcca
    26881 tcttttttgg gtaaaaattt atccagtttt
//
LOCUS       DAEMAE010000032        26305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEMAE010000032 DAEMAE010000000
VERSION     DAEMAE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.032"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            635..3685
                     /locus_tag="KPH93_001088"
     CDS             635..3685
                     /locus_tag="KPH93_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG1687921.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGYMKVEGETAYCIDINTNFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHFGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFIKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     IYSLKVEVGKTAILKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYIAKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSSDLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGVGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELLIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIVKIHTTATDKDGNKELEAGKDVTIIDTVI
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVKSDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKTKVPEEPKKPETPQTPD
                     TPHKTDSPKTGDSTNLYGLLALLLTSGAGLAGIFFCKRRKMKKS"
     gene            3922..4248
                     /locus_tag="KPH93_001089"
     CDS             3922..4248
                     /locus_tag="KPH93_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG1687922.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLLADDI
                     VAVEEK"
     gene            4264..4647
                     /locus_tag="KPH93_001090"
     CDS             4264..4647
                     /locus_tag="KPH93_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG1687923.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKSNQNAGNTQNHTPQGQPKK"
     gene            4749..5513
                     /locus_tag="KPH93_001091"
     CDS             4749..5513
                     /locus_tag="KPH93_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1687924.1"
                     /translation="MDSWFTVEQIDQDTFVISEYKHWEETHCYLLCGTKRAILIDTGL
                     GVSNIREVIDGLTKLPVTVITTHVHWDHIGGHKYFRSIAVHEAEKEWLSVKFPIPLQV
                     VKNNIMCNPCDFPSDFKVEKYQIFQGVPQMILHDEDCIDLGNRKLVVIHTPGHSPGHC
                     CFYEADRKYLYSGDLIYSGCLDAFYPSTNPQEFWKSVRKIQSLKISRILPGHHHLSIP
                     VTIIDKIETAFHNLSNEGKLKQGNGIFSYEGFQIHI"
     gene            5612..7006
                     /locus_tag="KPH93_001092"
     CDS             5612..7006
                     /locus_tag="KPH93_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687925.1"
                     /translation="MRMIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNSKQLM
                     CTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDSIFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGPLAQARQ
                     DVTATCEAKGDGTD"
     gene            6976..7161
                     /locus_tag="KPH93_001093"
                     /pseudo
     CDS             6976..7161
                     /locus_tag="KPH93_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7184..8389
                     /locus_tag="KPH93_001094"
     CDS             7184..8389
                     /locus_tag="KPH93_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG1687926.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            8432..8653
                     /locus_tag="KPH93_001095"
     CDS             8432..8653
                     /locus_tag="KPH93_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687927.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            8770..9267
                     /locus_tag="KPH93_001096"
     CDS             8770..9267
                     /locus_tag="KPH93_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1687928.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            9242..9748
                     /locus_tag="KPH93_001097"
     CDS             9242..9748
                     /locus_tag="KPH93_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687929.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            9732..12179
                     /locus_tag="KPH93_001098"
     CDS             9732..12179
                     /locus_tag="KPH93_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1687930.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            12182..14359
                     /locus_tag="KPH93_001099"
     CDS             12182..14359
                     /locus_tag="KPH93_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1687931.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            14356..15357
                     /locus_tag="KPH93_001100"
     CDS             14356..15357
                     /locus_tag="KPH93_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG1687932.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            15354..16289
                     /locus_tag="KPH93_001101"
     CDS             15354..16289
                     /locus_tag="KPH93_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1687933.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            16532..16648
                     /locus_tag="KPH93_001102"
     CDS             16532..16648
                     /locus_tag="KPH93_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBG1687934.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            16664..18583
                     /gene="tet(M)"
                     /locus_tag="KPH93_001103"
     CDS             16664..18583
                     /gene="tet(M)"
                     /locus_tag="KPH93_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBG1687935.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            18702..18869
                     /locus_tag="KPH93_001104"
     CDS             18702..18869
                     /locus_tag="KPH93_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG1687936.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(18929..19282)
                     /locus_tag="KPH93_001105"
     CDS             complement(18929..19282)
                     /locus_tag="KPH93_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687937.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            19787..20209
                     /locus_tag="KPH93_001106"
     CDS             19787..20209
                     /locus_tag="KPH93_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1687938.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            20206..20436
                     /locus_tag="KPH93_001107"
     CDS             20206..20436
                     /locus_tag="KPH93_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1687939.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            20897..21100
                     /locus_tag="KPH93_001108"
     CDS             20897..21100
                     /locus_tag="KPH93_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG1687940.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            21182..22399
                     /locus_tag="KPH93_001109"
     CDS             21182..22399
                     /locus_tag="KPH93_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1687941.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            22579..24345
                     /locus_tag="KPH93_001110"
                     /pseudo
     CDS             22579..24345
                     /locus_tag="KPH93_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
     gene            complement(24580..26178)
                     /locus_tag="KPH93_001111"
     CDS             complement(24580..26178)
                     /locus_tag="KPH93_001111"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG1687942.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDVKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMGGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLVLAVTDIIKEGSYLIYKAEDSLIANAFGIEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT         8752 a         4645 c         5449 g         7459 t
ORIGIN      
        1 tttataactt tggtttacaa taatagagac aataaatcct aaaaaacata ataagaaaaa
       61 agtgcttaat attttagaaa gactggttta gtttacacca gctaagccag tcaaggaata
      121 atttaaagtg taatatataa taatgaaaaa gaacatgcta ttagaagttt ctaatgacat
      181 gttctttttt tgtagaaatc agcattatta tataaaaatt taagactgta aattttaatt
      241 gtgttgtaac taagtatgta ttttaaacat agtataatat agacccaaaa tggagggatt
      301 ataaatgaga aatattatac tttatttaaa tgatgatact tttatatcta aaaacacaat
      361 aaaaatctga ttatccgcta aaacacagta tctatcagca ttttcaagaa aacaaataca
      421 tcaatttact acttatttac tactaacgaa tgagccttga tacgcaagtt ttcctagata
      481 tgcaaagata tggtacagca catcagtatt aaaataagaa aaaattgaat aactcataga
      541 ctatggaacg cctaacatga cgttcctttt ctatttccag ccgttcttat tggacggcta
      601 ttttattacc caaaatgaaa ggagcattag atttatgaaa aagatgttaa aacgattgtg
      661 tacgggcttc ttagctcttg caactgtcgt tactgcttta ccgactacac ccgtccatgc
      721 agaaagtaag caatactgga cggaaagtgc agagcgtgtc ggtatcattg aaaaagtaat
      781 gaatgacggt tctatcggtt cgacattcaa tgagggctat atgaaagtcg agggcgaaac
      841 tgcctattgt atcgacatta acacaaactt taagaacggt tacaagacca gagctgacgc
      901 aagctcacgc atgagtgctg accagatttc agatgtggcg ttatccttag agtatgtcaa
      961 acagtatggc gaagctcaca aggaactgaa ctacaaacaa gtctatctgt tggaacaatg
     1021 tgtagtctgg cagagattga gcgtacattt tggctggcaa tgtgataacg tgcgagcttc
     1081 ttatgatgaa attccaaagg caacacagga cgaagttttc tctggtgcaa aagcctttat
     1141 caaagaaaat aaaggacgct atgaatgtgg cggttatatc tactctggcg aggggcagga
     1201 attgggacaa ttctgggcga agctgaatgt cggaaatgca aaactgcaaa agacttccag
     1261 taataccagc attaccgacg gtaacgggaa ttactctatt gcaggtgcaa cctatggtgt
     1321 cttttctgat aaggactgta cgaaacagct tgccaccctt acgactgatg aaaacggaaa
     1381 tacagatgtt gtagaggtaa aggctggcac agtttatatc aaggagttat ccgcaccagc
     1441 aggatataaa gtagataaaa ccatatattc cttaaaggtt gaagttggaa agacagcaat
     1501 tttgaaagta tcagatacgc caaaagtaac ggacactttg attgagcttt tcaagattga
     1561 tatggaaaca cagaaagaca atccgcaagg gaacgcttct ttagcaggtg cggaatttac
     1621 atggaagtat tatgcaggct tctataataa agacaatctc cctgccgaag ctactcgtac
     1681 atgggttaca aagacaatcg ctgaaacaga cagcgacggg acaactcact acatcgcaaa
     1741 attagcggac gcatacaagg tatctggcga tagcttctat atgcaggacg gcaaggcggt
     1801 tcttccactt ggaacgctaa ccgttgagga aacgaaagct ccaaacggct acttgttaga
     1861 cggtgcatat atgcaggctg gcgataagtc cgaacagata aaaggcttat atgtaacaca
     1921 gattacagag gacggcgacc ttgccgtact tactggaagt aaccagtttt ctgtatcaga
     1981 caaggttatc cgtggtggtg tgaaaattca gaaacgagat ttagaaacgg gcgatacaaa
     2041 gccacaagga agtgctacct tgaaagatac tgcttttgac atcatttcct taaatgataa
     2101 tgcggtattg gttgagggca aattatacaa gaaaaatgaa gtggtaaaaa caatccatac
     2161 cgatattgag ggtgtcgctt ctacttcttc tgacctttta ccttatggaa aattccgtat
     2221 cgttgaaagt gaagctcccg acggttactt aactgacggt gcaaagccga ttgattttac
     2281 aatcacagaa aatggaaaaa tcgtggactt aaccgacgaa gctcattcta tctataatca
     2341 gattaagcgt ggagatattg agggtgtaaa aatcggtgta ggtacacaca agcgtcttgc
     2401 tgatgttccg tttcggatca caagcaagac gacgggcgaa aatcatgtag tggtaactga
     2461 tgataacggg caattctcca cttctgctga ctgggcttct cataagcaca ataccaacgc
     2521 aggcaagacc agcgaggacg gtgtgtggtt tggaacttct gaaccagacg acagcaaggg
     2581 tgcattacct tatgatacct acatcattga agaattacgc tctgatagta acaaaggctt
     2641 tgaacttatc ccaccttttg aaatcgtggt atcaagaaat aaccttgtga ttgatttagg
     2701 aacgctgact gatgaatacg aaaaagaaat ttctatccat accacagcga ccagcaagga
     2761 cggcgaaaaa actatccttg caggaaaaga gattacgatt gttgatactg taaaattgga
     2821 cggacttaca aaaggcacaa aatatcagct caaaggctgg cagatgttaa aggaagaaaa
     2881 cgcagaactt cttattgacg ggaaacgtgt agaaaacgat tatacctttg tcgctgatga
     2941 tgaggaaatg aaagtggaaa tttcctatac attcaatgcg tctgctttag gtggcaaaaa
     3001 ccttgttacc tttgaagaat tgtatgattt aagcaatcca gacgaacccg taaaagttgc
     3061 ggaacataaa gacattgagg acgacggaca gacggtactt atcacagagc gtatcgtcaa
     3121 aattcatacg actgctacgg ataaggacgg caacaaagag cttgaagctg gaaaagacgt
     3181 tacaatcatt gataccgtaa tcttagaggg cttagaagtc ggtacacagt acaaacttgt
     3241 gggctggcag atgttgaaag aagaaaatgc agaacttctt atcaatggaa aacgtgtgaa
     3301 aagtgattac acttttactg ctgacagcga aactatgaaa gtggaagttg cctttacctt
     3361 tgacgctact tctcttgacg gcaaacagct tgtaactttt gaagaattgt acgatttaag
     3421 caatccagac gagccgaaga aagttaccga gcataaggat attgaggata agggacagac
     3481 gattaccttt aaggaaaaaa ccaaagtccc agaagaacca aagaaacccg aaacaccaca
     3541 gacaccagat acaccgcata aaacagacag tccaaaaact ggcgatagca caaaccttta
     3601 cggactgctt gcacttcttt tgacttctgg tgctggactg gcaggtatct tcttctgcaa
     3661 acgccgtaaa atgaagaaat cataaggcgg taacggtatg agaaaagaaa aatcacgttc
     3721 tccgccgata ccgtgtcggc agacttccgc ttattctgga ttgtctgcta cttacctgca
     3781 agaacacgga tagtcaaggg tgcgtaagca ttgattttac cctttactat ctgggggatt
     3841 gctggtactt cccaaaccgc cagaataaga aatcacaatc aaaaaactta tcaaaaatag
     3901 aaagaaatga ggtaaaacac tatggaaatg aaatatgtcg tgccagatat ggcacagtct
     3961 tttggaactc ttgaatttgc aggcgaaagt gagccagtct ttgaaagaga taaaaataac
     4021 cgcagggtct tagccagacg aagctataac ctttattctg acgtacaaaa aggcgaaaat
     4081 gttgtggtag aaattcccgt gcaggctggc gaaaagcatt tcaagtatga acagaaagta
     4141 aaacttgtca atccgaagtt atatggcaga ggttacgcaa tcggggatat gggacatacc
     4201 gattatgtgt tgcttgctga tgatattgta gcagttgaag aaaagtaaag gagtgaagaa
     4261 tttatgagat tatcaaacgg gtttgttatt gacaaagaga aaacatttgg agaattaaaa
     4321 tttacagcgg tgcgagatgt gttcttgcag aatgaggacg ggacaccgag tacccagctt
     4381 aaaaagcgta tctatgattt gaagtgcagt ctgcatggtg gaattatccc cgtgtccgta
     4441 ccgccagaag taccgttaag ggaatttccg tacaatgcgg ttgtggagct tgtcaatcca
     4501 gtagccgata cggtatcaag aaaaacctat acgggtgcag atgtggactg gtatgtaaag
     4561 gcagaggata ttgtattgaa gaataaaagc aaccagaacg caggcaatac acagaaccat
     4621 acaccgcagg gacaaccgaa aaaatagact tgctgaatag attttcgttg tagaggatat
     4681 acggaactgt tataatatgg gtatgaagaa agcgacaaat taaaattttt agggagtgag
     4741 aatagtatat ggacagttgg tttaccgtcg aacaaataga ccaagataca ttcgtcatca
     4801 gcgaatacaa acactgggaa gaaacacatt gttatctatt gtgtgggaca aaaagagcca
     4861 ttctgattga tacaggttta ggtgtttcca acatcagaga ggttattgat ggattgacga
     4921 aactgcccgt tacagtaatc actactcatg ttcattggga tcatatcggt ggacataaat
     4981 attttcggtc tattgcagtt catgaagcag aaaaagaatg gctatcggtc aaattcccta
     5041 tacctttgca agtggtaaaa aacaatatta tgtgcaatcc atgtgatttc ccctcggatt
     5101 ttaaggtaga gaaatatcag atttttcaag gggttccgca aatgatttta catgatgaag
     5161 attgtattga cttagggaac agaaagttgg tagttattca cacgcccggt cattctcccg
     5221 gacattgttg tttctatgaa gcggatagaa aatatctgta ttccggcgat ttgatatata
     5281 gcggttgcct tgatgctttt tatccatcaa caaacccaca agaattttgg aagtctgtta
     5341 gaaaaattca atctttaaag ataagtcgaa ttttgccagg acatcatcat ttatctattc
     5401 cagttactat aattgacaag atagagacag cttttcacaa cttgtcaaat gaagggaaat
     5461 taaaacaagg gaatggtata ttcagctatg aggggtttca aatccatatc tgaaatgcaa
     5521 tcttagtttt acaattttat atccatacac acaaagcagt tagtcttagg attaactgtt
     5581 tttttcatac ccagaaagga gtgattgatt tatgcgtatg atttggaaca aaggacaccg
     5641 tatcagagcc agcgacaagc accttgtcta ccacttttcc atagggacgc ttctgtttgt
     5701 gttcgtggcg gttctcctgc tactgaatag caaacagctc atgtgtaccg attgggagca
     5761 tttcagcttg ttagagaacg gcttgacgct ttccccttac aacttcataa ccatactgat
     5821 agcgactggt gtttgtgcat tggtcgcttt tctgtattac cgcttctgtt atgacagttt
     5881 caagaagctc ctgcaccgtc aaaagctggc aagaatggta ctggaaaata agtggtatga
     5941 agctgatacc gtacaagaca gcattttttt tactgacctg caaagcagat caagggaaaa
     6001 aatcgtctgg tttccaaaga tttattatca aatggaaaag ggactgcttc atatccgctg
     6061 tgaaattacg ctggggaaat atcaagacca gcttttacgg ttagaggata aattggaaag
     6121 tggcttgtat tgtgagctga ccgacaagac cctgcatgac ggctatatcg aatataccct
     6181 gctttatgat atgatagcaa accgcattac cattgatgaa gtacgggcag aaaacggctg
     6241 tcttagactg atgaaaaatc ttgtgtggga atatgacgca ctccctcacg cccttatcgc
     6301 tggtggaact ggtggcggta aaacatactt tttgctgaca ctcattgaag ccttactgca
     6361 taccaacgct gtcctttaca tcttagaccc gaaaaatgcg gacttagcag acttagggac
     6421 agttatggga aatgtgtatc ataccaaaga agaaatgatt gattgcgtca atgcctttta
     6481 tgagggaatg gtacagcgaa gcgaggaaat gaagcgacac ccagattata agacgggcga
     6541 aaactacgcc tatctgggac tgccaccctg ctttcttatc tttgatgaat atgtggcgtt
     6601 ttttgaaatg ctggggacaa aagaaagcgt gagcttactt agccagttaa agaaaatcgt
     6661 catgctgggg cgacaagcag gttattttct tattgttgcc tgccagcgtc cagacgcaaa
     6721 gtatttctcg gacggtatca gagataactt caatttccgt gtgggacttg ggcgtatcag
     6781 cgaattaggt tatggtatgc tgttcggttc ggatgtgaaa aagcagtttt tccagaagcg
     6841 tatcaaaggg cgtggctatt gtgatgtagg gacaagtgtt atcagcgaat tttacacgcc
     6901 tttagtcccg aaaggacatg attttttgca gactatcggc cctcttgcac aagcaaggca
     6961 ggacgtgacg gcgacgtgcg aagcgaaagg cgacggcacg gactagccgt tgctggtgtg
     7021 gcgtaagcca cgccagcagg taaaacccct cggtatctaa caggggggta caaatcgaca
     7081 ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac aaaatttcag
     7141 cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg gatttttact
     7201 gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg gactatctca
     7261 aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata ttgaaacagg
     7321 aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg aacgcttcaa
     7381 tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg attcgctttc cgacaacaga
     7441 cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc ttcatgagga
     7501 ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg tcctttgctc
     7561 ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca gacaatttga
     7621 aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg ttttggtggc
     7681 tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga ttttgaatat
     7741 ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc gcagttttaa
     7801 aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct
     7861 ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg actacgagca
     7921 gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg
     7981 attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg acaatccaga
     8041 gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag acgattccaa
     8101 acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga acaatcgtga
     8161 acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc tgaactggct
     8221 atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac
     8281 gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca agcagatttt
     8341 gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac tcaaatacaa
     8401 attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc tttataactg
     8461 gtttctatca aacgctcaat cactggtgct tttagcaatc gttgtgattg gcttgtatct
     8521 tggcttcaag cgtgagttta gcaaactgat tggcttttta attattgcga ttattgcggt
     8581 tggcttagtc ttcaacgctg ctggagtaaa agacatttta ctagagctat tcaatcgcat
     8641 tattggtgct taaataaaac cgttcttttg tggaatataa gtggttttct tatgttccgc
     8701 aaaggaatgg tacaccaaac gaagtgcggt agggattttt gaatctctac aaagaaagga
     8761 cgtgaatata tggacgatat gcaagtctat attgcgaatt taggcaaata caatgagggc
     8821 gaattggtcg gtgcgtggtt tacctttccc attgactttg aggaagtcaa agagaaaatc
     8881 ggcttgaatg atgaatatga ggaatacgcc attcatgact acgagttacc ctttacggtt
     8941 gacgaataca cttccattgg cgaactcaat cgactatggg aaatggtatc ggaattaccc
     9001 gaagaattac aatcggagct atctgctctg ctcactcatt tttcaagcat tgaagaacta
     9061 agcgaacatc aagaggatat tatcattcat tccgattgtg atgatatgta tgacgtggca
     9121 cgctactaca ttgaagaaac gggtgcttta ggcgaagtac cagctagtct tcaaaactat
     9181 attgattatc aagcctatgg tcgggattta gacctttcag gaacgtttat ctcaaccaat
     9241 catgggattt ttgaaatcgt ctattaaatc tgtcggtaca ttactactgg cagattttct
     9301 attttacggg gtggctcaat cagctacccc tattttttat gaaaggattg attacatgaa
     9361 gaaaatacga agctatacca gtatctggtc tgtggaaaag gtactgtatt ctatcaatga
     9421 ttttagactt ccgtttccca taacctttac gcaaatgaca tggtttgtcg tgtcactctt
     9481 tgcagtgatg atacttggca acttgccccc tctttccatg atagagggag catttctcaa
     9541 atactttggg attcctgtgg ctttcacatg gtttatgtct acaaaaactt ttgatggtaa
     9601 aaagccttat ggatttttga agtctgtcat tgcttatgca ctgcgaccaa agctgaccta
     9661 tgcaggaaaa aaagtaacgc ttggcagaaa ccagccacaa gaagccatta cagcagttag
     9721 gagtgaattt tatggcatat ccaattaaat acattgaaaa caatctcgtc tggaataaag
     9781 acggggaatg ttatgcttac tatgagcttg ttccttacaa ttactcattt ctaagtccag
     9841 aacagaaaat acaagtgcat gattctttca gacagcttat cgcacaaaat cgtgatggca
     9901 aaattcatgc tttacaaatc agtacagaat ccagcatacg ttctgcacaa gagcgttcca
     9961 aaaatgaagt cactggcaag ctcaaagcgg ttgcctatga caaaatcgac caacagacag
    10021 acgctttaat atccatgatt ggcgaaaatc aagtgaacta ccgtttcttt atcggcttta
    10081 agttgcttct caacgatcag gagttttcta tgaaaagtct taccgttgaa gcaaaaaatg
    10141 ctttgtctga ttttgtctat gatgtgaacc ataagctgat gggcgatttt gttagtatga
    10201 gtaatgatga aatcctgcgt tttcagaaga tggaaaagct cttagaaaat aaaatctctc
    10261 gtcgtttcaa aatccgcagg ttagataagg acgacttcgg ctatctgatt gaacaccttt
    10321 acggacagac aggcactgcc tatgaagagt atgagtacca tctatcaaag aaaaagctgg
    10381 ataatgaaac gctgattaaa tactatgact tgattaagcc tactcgctgt ttggtggaag
    10441 aaaaacagcg atatttgaaa atccagcagg aagatgaaac cgtctatgta gcttacttta
    10501 ccattaacag cattgtcgga gaactggact tcccgtcctc tgaaatcttc tactaccagc
    10561 aacagcaatt tacattcccg attgatacgt caatgaatgt ggaaattgta gcgaatcgta
    10621 aagccctatc tactgtccgc aataaaaaga aagaactgaa agacttggat aaccacgctt
    10681 ggcaaagtga taatgaaacc agctccaatg tggcggaagc tctggaaagt gtgaatgagc
    10741 tggaaaccaa tttagaccaa agcaaggaat ctatgtacaa gctgtcttat gtggtaaggg
    10801 tatcagcaaa tgatcttgac gaactcaaac gtcgttgtaa tgaagtgaaa gatttttatg
    10861 acgatttaag cgtaaaactg gtacgaccat ttggggatat gctcggctta catgaagaat
    10921 ttttacctgc cagcaagcgt tatatgaatg attatattca atacgtgacc tctgatttcc
    10981 tcgctggttt aggttttggt gctactcaaa tgctggggga aaatgagggg atttatgttg
    11041 gctacagctt agatactgga cgcaatgtct atctgaaacc tgctcttgcc agtcaagggg
    11101 ttaagggttc agtaaccaat gcgttagcgt cggcttttgt tggttcgctg ggtggtggta
    11161 aatcctttgc gaataacctt atcgtctatt atgcggtgct ttatggggca caagcagtga
    11221 ttgtagaccc aaaagcagaa cgtggcagat ggaaagaaac cttgccagag atttcccatg
    11281 aaatcaatat cgtcactctg acttctgatg agaaaaacaa aggcttactt gacccttatg
    11341 tgattatgaa aaatcccaaa gattctgaat cactggctat tgatattctg acattcctta
    11401 cggggatttc ctctcgtgat ggggaacgct tcccaatcct tagaaaagcc attcgtgcag
    11461 taaccaatag tgaagtacga gggttgatga aagtgattga ggaattacgg gttgagaata
    11521 cgccactaag taccagtata gccgaccata tcgaaagttt tacagactat gactttgcac
    11581 atttattatt cagtaatggt tatgtggagc agtctatcag cttagaaaaa caactgaaca
    11641 ttatacaggt tgcggacttg gtacttcccg acaaggaaac ttcctttgag gaatatacca
    11701 ctatggagct tttatccgtt gctatgctga ttgtcattag tacctttgct ttagacttta
    11761 tccatacaga ccgaagcatt ttcaagattg tagatttaga cgaagcatgg agctttttac
    11821 aggtagcaca aggaaaaaca ctatctatga agctggttcg ggctggtcgt gctatgaacg
    11881 ctggggtata tttcgtgacc caaaatacag acgacctctt agatgaaaaa ctgaaaaata
    11941 acctcggctt aaaatttgca tttcgttcca ctgaccttaa cgagattaaa aagaccttag
    12001 ccttttttgg tgtagaccca gaggacgaaa acaatcagaa gcgattgcgt gatttggaaa
    12061 acgggcaatg ccttatcagt gatttatatg gtcgtgtcgg tgtgatacag ttccaccctg
    12121 tatttgaaga actgctccat gcctttgata ccagaccacc tgtgcgaaaa gaggtgtaaa
    12181 tgtgaaacca tcaatagtaa acagaataaa atcaaactgg acgctgaaac gtctaggtaa
    12241 agtggcaatg acagtggctt tcacacttgt gattgccatt tttcttttag ccatgctggg
    12301 aacggtggtt caagctgcgg gcttggtaga tgatacggtc aatgtggcaa atgaatacag
    12361 ccgataccca cttgaaaact atcaactgga tttttatgtg gataatagct ggggctggct
    12421 tccgtggaac tggtcggacg ggattggaaa acaggtcatg tatggactat atgccattac
    12481 caattttatt tggacaatca gtttgtatgt ttccaatgcg acaggttact tagtacagga
    12541 agcctattcc ttagacttca tttccgctac agcagattcc attggtaaga atatgcagac
    12601 cttagctggt gtgagtgcaa acggattttc aacagagggt ttctatgttg gattcctctt
    12661 actcttgatt ttggttcttg gggtttatgt tgcctatacg ggactgataa agagagaaac
    12721 cacaaaggca attcatgcca ttatgaattt tgtgctggtg tttatcctat cggcttcctt
    12781 tattgcctac gctcccgact acattaaaaa aatcaatgac ttttcatcag acatcagtaa
    12841 tgccagttta tcacttggca cgaagattgt catgccccat tccgatagtc aaggcaagga
    12901 cagcgtggac ttaatcagag atagcctgtt ttccatacag gttcagcaac cgtggctact
    12961 gcttcaatac aacagttcag acattgaaag tatcggtatt gaccgtgtgg aaagcctgct
    13021 ctccaccagc ccagattcca acaatggcga agacagagaa aaaattgttg cggaagaaat
    13081 tgaagacaga agcaatacca atctaaccat tacaaagacc attaaccgtt taggtacagt
    13141 cttcttccta tttgtcttca atattgggat ttccatattt gtattcctat taacaggaat
    13201 catgattttc tcgcaggtac tttttatcat ctatgctatg tttctgcctg tgagctttat
    13261 tttaagcatg attccatcat ttgatggtat gtcaaaacga gccataacaa agctctttaa
    13321 taccattttg acacgagctg gaatcacatt gattattacg acagcattta gtatttcaac
    13381 catgctctat accttatcgg ctggttatcc gttctttttg attgcttttc tacagattgt
    13441 gacctttgca ggaatctact tcaagctggg cgatttaatg agtatgtttt ctctacagag
    13501 taacgattct caaagtgtgg gaagtcgtgt gatgagaaaa cctcgtatgc ttatgcacgc
    13561 tcacatgcac cgtctacagc ggaaacttgg acgttccatg actactctag gggctgggtc
    13621 tgccattgtt acaggtaaaa aaggacagtc gggttcgggg agttctgcaa ggacacaagc
    13681 agatcactcc cgaccagacg gaaaggaaaa atcaacactt ggaaaacgta tcggtcaaac
    13741 catcggtaca gtagctgata ccaaagacag aatggtagac actgctagtg gtttgaaaga
    13801 acaggttaaa gatttgccga ccaatgcaag atatgcagta tatcaaggaa aatccaaagt
    13861 aaaagagaat gtccgtgatt taaccagtag tatttctcaa accaaagcgg acagagccag
    13921 tggacgcaag gaacagcagg aacaaaggcg aaaaaccatt gcgaagcgtc gctctgaaat
    13981 ggaacaggtc aaacagaaaa aacagcctgc ttcttctgtt catgaaagac cgactacaag
    14041 acaagaacaa tatcatgatg aacagacctc aaaacagtct aatattcaga cttcatataa
    14101 ggaatctcaa caagccaaac aagagcgtcc agcagttaag tccgattttt caagtccaaa
    14161 agtggaacgc caaggcaata ccgttcaaga aaaaaccgtt caaaagccag caacttcaac
    14221 cactacagca gatagaactt cacaacgtcc aatcacaaaa gaacgtccgt ctactgttca
    14281 aagagtacca ctacaaaata caagaagtag accaccaatc aaaaccgcca ccattaagaa
    14341 agtcggtaag aaaccatgaa gttgaaaact ttagtgattg gtggttctgg attattcttg
    14401 atggtcttct cactgcttct gtttgttgcc attttatttt cagatgaaca ggacagcgga
    14461 atttccaata ttcattatgg aggtgtgaat gtttccgcag aagtgctggc tcataagcct
    14521 atggtagaaa aatatgccaa agaatatggc gttgaagaat atgtcaacat acttcttgcg
    14581 attatacagg tggaatcggg cggtactgcg gaagatgtta tgcagtcctc ggaatccctc
    14641 ggtcttccac ctaattcatt gagtacagaa gaatccatta agcaaggtgt gaagtatttc
    14701 agtgaattat tagccagtag cgaaaggctc agtgtagatt tagaatcggt tatccagtcc
    14761 tacaattatg gtggtggttt cttagggtat gtggctaatc gtggaaataa atataccttt
    14821 gaactggctc aaagtttctc aaaagagtat tcaggtggcg aaaaagtgtc ttaccccaat
    14881 cccatagcca tacctatcaa tgggggctgg cgatacaact atggcaatat gttttatgtg
    14941 caactggtaa cgcagtatct tgtcacaaca gagtttgatg atgatacggt acaagccatc
    15001 atggacgaag cactgaaata tgagggctgg cgatacgttt acggtggagc ttccccgact
    15061 acttcttttg attgtagcgg actgacacaa tggacgtatg gaaaagctgg aattaactta
    15121 ccacgaaccg cacaacagca atatgatgtg acccagcata tcccactatc ggaagcacaa
    15181 gctggcgatt tggttttctt tcattctacc tataacgctg gctcttatat tactcatgtt
    15241 gggatatacc ttggcaataa ccgtatgttt catgcaggcg acccaatcgg ttatgccgac
    15301 ttaacaagcc cctactggca acagcattta gtgggagcag gacgaatcaa acaatgagaa
    15361 aggaagattt aatgatgaaa tttagaaaaa atcagaataa agaaaaacag ataccaaagg
    15421 aaaagaaacc tcgtgtctac tataaggtca atcctcataa aaaggttgtg attgccttgt
    15481 gggtactttt agggcttagt ttcagctttg cgatattcaa gcactttaca gctatagata
    15541 ctcatactat tcacgaaaca actatcatag aaaaggaata cgttgatact catcatgtag
    15601 aaaattttgt agagaacttt gcgaaagtct actattcatg ggagcaatcc gataagtcca
    15661 ttgataatcg aatggaaagt ctaaaaggct atctgacaga tgaacttcaa gctctcaatg
    15721 ttgatacagt acgcaaagat attcctgtat cgtcttctgt aagaggattt cagatatgga
    15781 cggtagagcc aactggcgac aatgagttta atgtaaccta cagtgtagac cagctcatta
    15841 cagagggaga aaatacaaag accgtccact ctgcttatat agtgagtgtc tatgtagatg
    15901 gttctggaaa tatggtactg gttaagaatc cgaccattac caacatacct aagaaatcaa
    15961 gttataaacc aaaagccatt gaaagtgagg ggacggttga ttccattaca accaatgaaa
    16021 tcaatgagtt tttaacgacg ttcttcaagc tctatcctac agcgacagcc agtgaacttt
    16081 cctactatgt gaatgacggg atattaaaac caatcggaaa agagtacatc tttcaagaac
    16141 tggtaaatcc tattcacaat cgtaaggata atcaagtcac ggtatcgctg acagtggagt
    16201 atatcgacca gcagaccaaa gcaacgcagg tatctcaatt tgatttggta cttgaaaaga
    16261 acgggagtaa ttggaagatt atagaataac aaatattggt acattattac agctattttg
    16321 taatcacgta ctctctttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg
    16381 ctattattta agtatctatt taaaaggagt taataaatat gcggcaaggt attcttaaat
    16441 aaactgtcaa tttgatagtg ggaacaaata attggatgtc cttttttagg agggcttagt
    16501 tttttgtacc cagtttaaat acctttatca tgtgattcta aagtatccgg agaatatctg
    16561 tatgctttgt atgcctatgg ttatgcataa aaatcccagt gataagagta tttatcactg
    16621 ggatttttat gcccttttgg gcttttgaat ggaggaaaat cacatgaaaa ttattaatat
    16681 tggagtttta gctcatgttg atgcgggaaa aactacctta acagaaagct tattatataa
    16741 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
    16801 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
    16861 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
    16921 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
    16981 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
    17041 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
    17101 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
    17161 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
    17221 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
    17281 aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg
    17341 gattgataac cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtcc
    17401 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
    17461 atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa
    17521 agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga
    17581 tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
    17641 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
    17701 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
    17761 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
    17821 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
    17881 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
    17941 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
    18001 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
    18061 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
    18121 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt acggtttata
    18181 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
    18241 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
    18301 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
    18361 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
    18421 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
    18481 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
    18541 tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta
    18601 tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa
    18661 gtgtaaagga ggagatagtt attataaact acaagtggat attgtgtcct gtatgtggaa
    18721 ataaaacacg attaaagata agggaagata ctgaattaaa aaaattcccc ctctattgtc
    18781 cgaaatgcag acaagaaaat ttaattgaaa taaagcagtt caaagtaact gtgattacag
    18841 agccagacgc aaagacgcag agccgataaa atgagattaa tacaatctca ttttatcggc
    18901 tctttccgtt atgtatggat tcttttaatt agtcttcgat gtttcttgct tcgttgatac
    18961 cgctggctaa agattccatt aaggatagtt ctttgtctgt aaagctatcc atgtatttct
    19021 ctatctgtaa tcgtcgggtg ctttttacca agttattagc aggtaagaaa aattcatcaa
    19081 cggaaacatg aagtaacgat acaaggtcat aaagaacttg tatgctgggg tgttgccctt
    19141 tattttcaat attagttaag taccgtgggt caatttcaat caatgctccc acttgttcac
    19201 gagttaaacc tcgtttcaat cgagcttctt taatggctaa accaaaggct ctaaaatcat
    19261 atttatcttc ttttttacgc atagtagacc acctctatac attttattgt tcctactgaa
    19321 ttaaaaacag gtatagaaaa acgtgttata tggtttatag gtttatattt aataaaaagc
    19381 actactaaac gccaataaaa aaaaccgtta tatggtagtg ctatttacgc tgttaaaata
    19441 ttgtatatta cttccaaatg gcggtttgtt ggaggtcaac gtcgccatga agtacatcat
    19501 atacaataaa tttccttaca ttgggttctt gtcaaaaaaa gtcgtctatc tgcaatagat
    19561 aagtacgtcc accaatgtgg ttttataaat catatagata gaataacaga agcatgtaaa
    19621 cagagaaata aatctgttta tatgcttttt tggctattca gaactttttt acaaagttta
    19681 tttatcagta atgcaacaaa tccccctttc acattgggac taagagtgaa aggagataaa
    19741 cgagcaaggc tcacttcctt tcctagacag aaagggggtg agaaacatga aaccatcttc
    19801 ttttcagacc acaatagaaa atcagtttga ctatatctgt aaacgtgcta tggaagacga
    19861 gcgaaagaat tatatgcttt atctttcaag gattgcaaag cgtgaggtgt ccttttcgga
    19921 tgttggcgat tatcttgtta gccagtttgc gacaacagat aactattcaa ctgactttca
    19981 gatttttaca ctcaatgggt tatcagtagg cgttgaaaat gatttgttga gtgaagcatt
    20041 acgtgagttg ccagacaaga aacgtgaaat tctactgctg ttttacttta tggacatgag
    20101 cgattcagaa attgcagacc tgttgaaatt gaaccgttct actgtctatc ggcatagaac
    20161 cagtggacta gccttaatta aaaagtttat ggaggaattt gaagaatgaa aacacaatat
    20221 cctatgattc cctttcctct cattgtaaag gcaacagatg gcgataccga agcgattaac
    20281 cagattctac atcattacag agggtacata acgaagcgtt ccctacgact tatgaaagat
    20341 gaatatggca atcaaagtat ggtcgttgat gaagtcttac gtggaagaat ggaaaccaga
    20401 ctgattacaa agattttgtc atttgaaatt aagtaatatc ctctctcctt tcgtggaagc
    20461 gtgctaaacc attccacgct tcccgaacag ggaggtttgt tattccacca aagcatattg
    20521 agctttcaat gtgttttgat aggctaacga gccattgttc tttgaaaact gaataaaagt
    20581 aatcgaatac gtttcgataa gaaaagagcc aacggaacta accgccatga cctatcttat
    20641 aaagatagcg agcgattcat gttagtgatc cgagaagcaa tctttagcag gattgcctgc
    20701 aacgacattc ttatcgtgat aatgatactc ccatacagtc aatagtccga gcgtgataaa
    20761 accgtcgcag gcaatgagta tggctacatg agaaccatgc aggggtggaa ctcccgtgag
    20821 ctttgctaaa gctgttcgat tgctggtaaa acaactttta tgaaatccaa ataagtgatt
    20881 tggaaaggag gattttatga agcagactga cattcctatt tgggaacgtt ataccctaac
    20941 cattgaagaa gcgtcaaaat attttcgtat tggcgaaaac aagctacgac gcttggcaga
    21001 ggaaaataaa aatgcaaatt ggctgattat gaatggcaat cgtattcaga ttaaacgaaa
    21061 acaatttgaa aaaattatag atacattgga cgcaatctag cgtcgccaaa gggtcttgta
    21121 tatgataaaa tagtattaag tcgtatcaag gctctttcca taaaggaaag gagcaaatgc
    21181 catgtcagaa aaaagacgtg acaataaagg tcgaatctta aagactggag agagccaacg
    21241 aaaagacgga agatacttat acaaatatat agattcattt ggagaaccgc aatttgttta
    21301 ctcgtggaaa cttgtggcta cagaccgagt accagcagga aagcgtgatt gtatctcact
    21361 tagagagaaa atcgcagagt tacagaaaga cattcatgat ggtattgatg ttgtaggaaa
    21421 gaaaatgaca ctctgccagc tttacgcaaa acagaacgct caaagaccaa aggttagaaa
    21481 aaacactgaa actggacgca aatatcttat ggatattttg aagaaagaca agttaggtgt
    21541 aagaagtatt gacagtatta agccatcaga cgctaaagaa tgggctatta gaatgagtga
    21601 aaatggttat gcttatcaaa ccatcaataa ctacaaacgt tctttaaagg cttcattcta
    21661 tattgctata caagatgatt gtgttcggaa gaatccattt gactttcaac tgaaagcagt
    21721 tcttgatgat gatactgtcc ctaagaccgt actaacagaa gaacaggaag aaaaactgtt
    21781 agcctttgca aaagctgata aaacctacag caaaaattat gatgaaattc tgatactctt
    21841 aaaaacaggt cttcgtattt cagagtttgg tggtttgaca cttccagatt tagattttga
    21901 gaatcgtctt gtcaatatag accatcagct attgagagat actgaaattg ggtactacat
    21961 tgaaacacca aagaccaaaa gtggcgaacg tcaagttcct atggttgaag aagcctatca
    22021 agcatttaag cgagtgttag cgaatcgaaa gaatgataag cgtgttgaga ttgatggata
    22081 tagtgatttc ctctttctta atagaaagaa ctatccaaaa gtggcaagtg attacaacgg
    22141 catgatgaaa ggtcttgtta agaaatacaa taagtataac gaggataaat tgccacacat
    22201 cactccacat agtttgcgac atacattctg taccaactat gcaaatgcag gaatgaatcc
    22261 aaaggcatta cagtacatta tgggacatgc taatatagcc atgacgctga actattacgc
    22321 acatgcaaca ttcgattctg caatggcaga aatgaaacgc ttgaataaag agaagcaaca
    22381 ggagcgtctt gttgcttagt agtacaaatg aatttactac ttatttacca cttctgacag
    22441 ctaagacatg aggaaatatg caaagaaacg tgaagtatct tcctacagta aaaatactcg
    22501 aaagcacata gaataaggct ttacgagcat ttaagaaaat ataaaaagat aattagaaat
    22561 ttatactttg tttaatttaa aaaatatcca gataaaaact ttagtaattt agattattgc
    22621 ttaataggaa gtaaatgttc aaatagtttt ataaaagaaa agttgattac tttttttaaa
    22681 gtgagaatac cagatatatt aaaagacaaa agtatattaa aagcagagtt atttattcat
    22741 attgattcaa ataagaatca tatttttaaa gaaaaagtag atattgaaat taaaagaata
    22801 agtgaatatt ataatttacg aactataaca tggaatgata gagtgtctat ggaaaatatc
    22861 agggggtatt taccaattgg tataagtgat acatccaact atatttgttt aaatattaca
    22921 ggaactataa aagcatgggc aatgaataaa tatcctaatt atgggttagc tttatcttta
    22981 aattaccctt atcagattct tgaatttaca tctagtagag gttgtaacaa accgtatata
    23041 cttgtaacat ttgaagatag aattatagat aattgttatc ctaaatgtga gtgtcctcca
    23101 attagaatta caggtccaat gggaccaaga ggagcgacag gaccaagagg agcaacaggt
    23161 ccgacaggaa gtacaggacc aacgggagaa ataggcccaa caggaaatac gggagcaaca
    23221 ggagcgacag gaagtatagg accaacggga gtaacaggtc caacaggaaa tacaggagta
    23281 acaggagaaa taggcccaac aggaaatacg ggagcgacag gaagtatagg accaacggga
    23341 gcaacaggtc caacaggaaa tacaggagcg acaggaagta taggaccaac gggagtaaca
    23401 ggcccaacag gaaatacggg agtgacagga agtatagggc caacgggagt aacaggtcca
    23461 acaggaaata caggagtaac aggagaaata gggccaacag gtccaacagg aaatacagga
    23521 gcgacaggaa gtatagggcc aacgggagta acaggcccaa caggaaatac gggagtgaca
    23581 ggaagtatag ggccgacggg agtaacaggt ccaacaggaa atacaggagt aacaggagaa
    23641 atagggccaa caggtccaac aggaaataca ggagtaacgg gagaaatagg accaatggga
    23701 gcaacaggtc cgacaggaaa tacaggagtg acaggaagta tagggccaac gggagtaaca
    23761 ggtccaacag gaaatacggg agtgacagga gaaatagggc caacaggaaa tacgggagcg
    23821 acaggaagta taggaccaat gggagcaaca ggtccgacag gagcaacagg cccaacggga
    23881 gcaacaggcc caacaggcaa ttcctcgcag ccagttgcta acttcctcgt aaatgcacca
    23941 tctccacaaa cactaaataa tggagatgct ataacaggtt ggcaaacaat aataggaaat
    24001 agctcaagta taacagtaga tgcaaatggt acgtttacag tacaagaaaa tggtgtgtat
    24061 tatatatcag tttcagtagc attgcaacca ggttcatcaa gtataaatca atattctttc
    24121 gctatcctat tcccaatttt aggaggaaaa gatttggcag gtcttactac tgaaccagga
    24181 ggtggaggaa tactttctgg atattttgct ggttttttat ttggtgggac gacttttact
    24241 ataaataatt tttcatctac aacagtaggg atacgaaatg ggcaatctgc aggaactgcg
    24301 gctactttga cgatatttag aatagctgat accgttatga cttaaaatgt gtctaaaata
    24361 atcttaaaaa ctatttagat tttatttaaa tgataaaagt atttttatat attgagtttt
    24421 acctatttta gaatgagtaa aataacaata ataataaaat atattcataa aaattttaaa
    24481 tttatggatt tttattttac tttattatca atatatgtat aataaaaaac tgtctcaaat
    24541 atagatttga gacagttttc gttatttaaa aattttatat tatttaaagt ttttgattgc
    24601 agtagttaaa ttaggaacta attgtttttt tcttgataca acacctggtg caaatgtacc
    24661 ttgaacacct tcaataccaa atgcgttagc aattaggcta tcctctgctt tatagattaa
    24721 ataagagcct tctttaatta tatccgtaac tgcaagaact aatttatcat aatcagttga
    24781 atttatataa gataaaaact catctttttt agcaaatata gagtctatgt ctaaagtaaa
    24841 tacttgtcca ataccaactc tatgtccacc catattaaat tctttaaaat ccatatttac
    24901 tatttcttct atagtatatt catctaaaga agtaccacat ttaaacatat ccatagcgta
    24961 tttttccatg tctacttttg ctattttact taattcttca caagctttct tatccatatc
    25021 agttgttgtt ggagacttaa ataataatgt atctgataat atagcagata aaagaagccc
    25081 agctatttca taaggtatct caacattatt ttctttgtac atttgataaa ttatagtact
    25141 attgcatcca acaggcataa ctctaaatga cataggaaca tcagtagaaa taccaccaag
    25201 tttatgatgg tcaattattt caactatgtt tgcttgttca attccatcag cactttgagc
    25261 atattcgtta tggtcaacta aaacaacatt ctttttagat gggtttaata gatgaccttt
    25321 tgaaactaaa cctaaaaact tattatcatc atctaatatt ggataagatc tgaagtttgt
    25381 ttctagcata atctctttta tttcatccat ataatcatct tctttgaatg taattaaatt
    25441 ttctgtagct tctatagagt ctaagaaact acattgattt attacattag aagttttata
    25501 attgtcactt tcaacagaaa caacagacac tccatttatc ttagcaagag ctagcaaatc
    25561 ttttgaaatc tctgtatgat tagtaagtat taaaagttta acttttgtat caatagcata
    25621 ttcaatacta tcatatctat ctccaacaat agctatgtct ccttctttta aagtctctat
    25681 tagagtctcc attccaaatg ataaagtgac tactctacct tttacatctt caccacagtc
    25741 cacaagaata gtaccattaa ggtttttaca aatgtttgat agtgatgtgt gtatagttgt
    25801 aaagtgttga tgaagtaggt ttttagctat ttctttcata gtaatgatac cagcaaaagt
    25861 tccatcatca taagttacag gtagtgtttt aacatttttt tcatccataa ggttataagc
    25921 ctctaatata gatgaagttg gtgttaaagg tactacttta tcgtaattta aatctctaac
    25981 ttgtattcta acattatcta aaatttcagg tgcatctacc ttaaagtaat ccaaggcata
    26041 tttagcttct tttctgatat ctcctaaagc ataagctgtt gcttttattc ctagtttgtt
    26101 ctttaaatgt gctaacgaaa ttgcagaaca tattgaatct gtatctggat ttttatgtcc
    26161 gaaaactaat aaactcattt taatactcct ttcaaaactt gaatataata ataattgata
    26221 aaaatattat atcaaaaggt agtgagtatt tgttgtaaaa agaaaaaaat ttagtgaaaa
    26281 aatattatat aaaatttatt tctta
//
LOCUS       DAEMAE010000033        26274 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEMAE010000033 DAEMAE010000000
VERSION     DAEMAE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.033"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..576
                     /locus_tag="KPH93_001112"
     CDS             19..576
                     /locus_tag="KPH93_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG1687943.1"
                     /translation="MISYLPNITIDTDKIKTIMINEVVPTNPNDDFYSIDKDADYLKT
                     TIPLFNNAGIVVSNINDILDMGIYITNAVKLPKSEYTITRDTIKLHMPILEEEIKLFK
                     NLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTYKLRNNELYYDNLRVFPSYIMTGG
                     NILIEKSKFEMASEDIKKMFEIING"
     gene            complement(826..1605)
                     /locus_tag="KPH93_001113"
     CDS             complement(826..1605)
                     /locus_tag="KPH93_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG1687944.1"
                     /translation="MNSKIYYFSGTGNSLVVSKTLKEMLNESKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KKEKKLSAGFLINMPGNALITPPDVEEERLRNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTDRKKYHHPDITVKDIFLDK"
     gene            complement(1673..2134)
                     /locus_tag="KPH93_001114"
     CDS             complement(1673..2134)
                     /locus_tag="KPH93_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1687945.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNKIANVYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(2110..2787)
                     /locus_tag="KPH93_001115"
     CDS             complement(2110..2787)
                     /locus_tag="KPH93_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1687946.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVFSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEAQGII"
     gene            3021..3407
                     /locus_tag="KPH93_001116"
     CDS             3021..3407
                     /locus_tag="KPH93_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1687947.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYIATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            3410..5209
                     /locus_tag="KPH93_001117"
     CDS             3410..5209
                     /locus_tag="KPH93_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG1687948.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLILPFVPLKYINLGNVFNYLYTFNQLGSNTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFTFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNRDILINYIMCFFQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSYIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHIESSALKFKSILIFTLVAL
                     IVFGNIPRLSIMADINEKYRPTNDKTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALFGLENGIITRDNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTKLKNIQTYLKQIGYGNHDVSGGISNFWLESSLKISPIEQVKLLESF
                     YTNKFKFEDKNIEAVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(5562..7649)
                     /locus_tag="KPH93_001118"
     CDS             complement(5562..7649)
                     /locus_tag="KPH93_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBG1687949.1"
                     /translation="MIKVVYMRKNKKTKKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENADGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNAEILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYLIIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            8010..8588
                     /locus_tag="KPH93_001119"
     CDS             8010..8588
                     /locus_tag="KPH93_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1687950.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYNSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            8581..9240
                     /locus_tag="KPH93_001120"
     CDS             8581..9240
                     /locus_tag="KPH93_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBG1687951.1"
                     /translation="MNKQKFHKIDLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(9452..11326)
                     /locus_tag="KPH93_001121"
     CDS             complement(9452..11326)
                     /locus_tag="KPH93_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687952.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVV
                     FCFIATCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVELNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(11331..12995)
                     /locus_tag="KPH93_001122"
     CDS             complement(11331..12995)
                     /locus_tag="KPH93_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG1687953.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIVSRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKAVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGEFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTISDLRKYILDSQMYVQQHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRNTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWSDKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKPVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(13009..13830)
                     /locus_tag="KPH93_001123"
     CDS             complement(13009..13830)
                     /locus_tag="KPH93_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1687954.1"
                     /translation="MKLYKLLILDIDGTLRDEVDGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKKVVCLLKKREVAF
                     SIESREKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIQRLQERLGITQKET
                     ICFGDGQNDIVMFQASDITIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(13929..14693)
                     /locus_tag="KPH93_001124"
     CDS             complement(13929..14693)
                     /locus_tag="KPH93_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1687955.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKKMKMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(14690..15439)
                     /locus_tag="KPH93_001125"
     CDS             complement(14690..15439)
                     /locus_tag="KPH93_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1687956.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(15721..16773)
                     /locus_tag="KPH93_001126"
     CDS             complement(15721..16773)
                     /locus_tag="KPH93_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687957.1"
                     /translation="MNVINVFNSNLKRNINKRLVFLVTLLFPIIIVILGVLANYISKP
                     SFNIGILDEYKNERVLKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFKLYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQWFISFVTYIEERDGSFFNMNYICILTSIIIILALL
                     AMKFIQRSKYSSNRNY"
     gene            complement(16773..17513)
                     /locus_tag="KPH93_001127"
     CDS             complement(16773..17513)
                     /locus_tag="KPH93_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687958.1"
                     /translation="MRYFLPLFKLGIKRRSKDFFILFYNIVFPVIIIMLLGYLTSKSY
                     GEEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTAVLSICNLITLAVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFITILTIKFFKKEVFI"
     gene            complement(17518..18276)
                     /locus_tag="KPH93_001128"
     CDS             complement(17518..18276)
                     /locus_tag="KPH93_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687959.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKRRTKDALEFVGLWERNKEFPLKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILKAIKDINANGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLEFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(18381..19103)
                     /locus_tag="KPH93_001129"
     CDS             complement(18381..19103)
                     /locus_tag="KPH93_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1687960.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLREEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFKSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            complement(19204..19809)
                     /locus_tag="KPH93_001130"
     CDS             complement(19204..19809)
                     /locus_tag="KPH93_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1687961.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            19988..20626
                     /locus_tag="KPH93_001131"
     CDS             19988..20626
                     /locus_tag="KPH93_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1687962.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDSTTMSELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQEMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEERFIETTLALLIDSIKA"
     gene            20771..21433
                     /locus_tag="KPH93_001132"
     CDS             20771..21433
                     /locus_tag="KPH93_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687963.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTITEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNTPCIKTLRGIGYRME
                     DS"
     gene            21433..22614
                     /locus_tag="KPH93_001133"
     CDS             21433..22614
                     /locus_tag="KPH93_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1687964.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKSTFWSKNFFYFLVVSFGI
                     ISTMTIVLYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDTSTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EGSPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGNGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(22795..25455)
                     /locus_tag="KPH93_001134"
     CDS             complement(22795..25455)
                     /locus_tag="KPH93_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1687965.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     IYRNATSEYWNHMPEKDTIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     STFIEKLDNIEGVKSSLYYQKMSAATWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFGEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLSISIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSRYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGLFKVNNRKDISRVREKLESICNKDYGSGDYSIEDVL
                     ESEKQDASGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPVSTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(25452..26135)
                     /locus_tag="KPH93_001135"
     CDS             complement(25452..26135)
                     /locus_tag="KPH93_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687966.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         9224 a         4285 c         3008 g         9757 t
ORIGIN      
        1 ctttttgatg acaacagaat aatatcctac cttcctaata taacaataga cacagacaaa
       61 ataaaaacta taatgattaa tgaagttgtc ccaactaacc ccaatgacga tttttatagt
      121 atagataaag atgctgatta tttaaaaact actattcctt tatttaacaa tgcaggaata
      181 gtagtatcta acataaatga tatcctagat atgggtatct atataactaa tgccgtaaaa
      241 cttcctaaat cagaatatac aataactcgt gatacaatta aattacatat gcctatttta
      301 gaagaagaaa ttaaattgtt taaaaaccta gaggtagtaa tgttaatggg agatgttgca
      361 aagaaatctt ttaatatgat tactaaaaaa catataaaaa agaatgtcat cccttccatt
      421 tcaacatata aacttcgtaa taatgaactt tattatgata atttaagagt atttccatca
      481 tatattatga ctggtggaaa catactaatt gaaaaatcaa aatttgaaat ggcatcagag
      541 gatataaaaa aaatgttcga gattataaac ggttgatatt gcaaaataat aaaaactaat
      601 ttagacaaat aataagtgcc tcaaaaactt ctatttatga ggcactttta ttttatatta
      661 gtgtaactat atactattaa cataatttgt tatttatatt gttcttttta atctctattc
      721 catttagtct aggtaacatt actctttaca agattacttt atatttctct tatatctcta
      781 acatttttat ttaataaaac aattattaat acaagtcaat tatatctatt tatctaaaaa
      841 tatatctttc acagtaatat ctggatggtg atatttcttc ctatccgtta aaaaagattt
      901 ttttacatta atagcctctt ttggacacca gtgaaaacaa gctaggcatc tttcacactc
      961 attaccccac tttggctttt tatcaactat acttatattc tctacaggac aaaccctttt
     1021 acatgtccca catccattac atttattttc tgcaaaaaat ttagtagttg caatttgcat
     1081 attcttacct actaaacgaa gtacaacact atctatatgt gactttaaat tgttttttcc
     1141 ttctatattt ccaatttcca agttattgat acattttgca atctcagcta ccctattagt
     1201 ctgatttctc aatctttctt cttcaacatc tggaggtgtt atcaaagcat ttccaggcat
     1261 attaattaaa aaaccagcag ataatttttt ctcctttttt ttaagtaact tatctatagt
     1321 aaataaacta tgtccagcta ccccattaca agtggcaatg gcatatatat gagtattaat
     1381 attaaaattt aatttctcta caaaagactt tactatatca ggaacatcag caaaataaac
     1441 aggaaaaaca attaccaata tatcttcatt tatgtctata ttattttctt ttctatgtat
     1501 agctaaaggt ataacttctt tactttcatt taacatttct ttcaaagtct ttgaaactac
     1561 tagcgaattt cctgtaccag aaaaataata aatcttacta ttcattctta tccctctttc
     1621 tatctttctg catcatcaat agttaaaaag tctcttaaaa taggatttat atttactttc
     1681 caattctatc caaaatatca gtaaacaaag gcaattcttc atcagagata gatttaaatg
     1741 tctctgttgc atatacattt gcaattttat ttaatggttc ttctaattct ctacctaaat
     1801 ctgttaggaa tatttgaaac ttacgtctat ctttaacatc tactttacgg acaattaatc
     1861 ccttattttt cataacatct aagattcttg tcgtattaga tttatcctta catgattttt
     1921 cacataaatc tatctgagaa attccatctt gctcccatag aaaccttaat aacgcataat
     1981 ggtcagttgt tatatcatat ccattttgtt taaacatctt ttttaaatca tcttttactt
     2041 tcaatgaagt acttactaac ttagttgtag cttcatatag tttctcaaaa tctttcattt
     2101 tacctccatt taaataatcc cttgcgcctc ccatctatca attattccct tatattgctc
     2161 aatgtcttta aaagccattg gctttaaaac agcatttata gcaataaaat tatatatttg
     2221 agaaaacaca ggtggatttt tcttttcctc tatgcaataa ataaattttt tggatacctt
     2281 ttgtatcttt gattctactt tttcagttat aggattttga ttaaatccag tttttataga
     2341 taaactacca caaacataac ctcccgaatc taagaccatt gattttataa aagaaattgc
     2401 ttttgaacca cgagcatttt catatgtagt aactgctata catggcttac gatacaataa
     2461 tctttcaagc atcatatgtg ctctatctgt aaacactttg aaaagtccag ttacactact
     2521 tccataagtt ggactaccaa atataacacc atcactctta gtaattatat catgaatata
     2581 ctctacttta tcattttgat taatacactt ccccatttta taacaattta aacaaccaat
     2641 acaataggat ggattgacct cacttaaatc aaaataatta atttttacat ctggtattaa
     2701 tctttctata tctttataca aataagtcaa aacttttgat gtagctccat tttttctagg
     2761 gcttccattt ataactgtaa ttatcatttc tatctctcct tttttagttg ttatatcaac
     2821 aataagtata tcaactatat ttttagctgt caatataaac cctttattta aagctatact
     2881 tacacagctt aaataaaagt tttatttaac tacatgtact tatttaatca actataaatg
     2941 aattattttt aataattgac aaactaaatt aatgataata tactttatgt attacaaatg
     3001 taagtagaag gaggtatagc atgaaaaagt taccacatat ttcagaagca gaataccaag
     3061 ttatgaaaat tatttggaaa tatgctccta tcagcactac tgaagtaatt gaaaagttgg
     3121 tagaaacaag tacatggagt cctaaaacaa ttcaaacaat gttattgaga ttagttaaaa
     3181 aaggagcttt aacatatgaa aaaaatagca gagtattcgt ttatactcca cttgttaaag
     3241 aagaagaata catagctaca gaaagtagtt cttttttaaa tcgtttttat aatggtactc
     3301 ttaattctat ggtactaaac tttttagaaa atgataaact atctgaagat gatattgagg
     3361 aactgagaga aatcttaaat aaaaggacta ctaaggggga taaataaata tgtatgcccc
     3421 attctttata cgatttttac tcagtacatt aaccttatca atattaattg ttgtaatttt
     3481 acttgccaaa aaagtattta aaaaacatat ttctgcaaag taccaataca atattggatt
     3541 tatattgctt ataatgttaa tacttccatt tgtccctctt aaatatatta atcttggaaa
     3601 tgtatttaac tatttatata cttttaatca acttggaagc aatactaata ggacttctat
     3661 gataaaaaat agtccaaatt ctataattaa taactcaaat gcattaaacg atttttcagt
     3721 atccctaaac caagttaatt ttgatagttt aaatatttta ttactaatat catggataat
     3781 tggaatttta atcatgatag ctattactat tcattgcaac attaaaataa aaaatatgaa
     3841 aaaatcaata caaattttaa gagatgagga gatagttttt acatttgaaa agtgtaaaaa
     3901 agatattggt ataaataagg attttatact atgcaaatca cctattataa atacacctat
     3961 tacatttggg tactttaaac cacacattat actaccagat aagtctatat ctaaattatc
     4021 tttaaaagat ataaaatata tcttacttca tgaattacag cattttaaaa atagagatat
     4081 actgataaat tatataatgt gcttttttca aatattatat tggtttaatc ctcttgtatg
     4141 gtatgcattt aaagaaatgc gtatagaccg tgaaattgca tgtgatattt cagttttaaa
     4201 aaagcttgat aaagatagtt atatagaata tggacaaact ctaataaact ttgtggataa
     4261 tatttcaaga ccatcaaatc ttacacttac atcagatatt ggtgggtcaa aaaaacaaat
     4321 aaaaaaacga ttattgaaga taatggattt tcatattgaa tcaagtgctt taaaattcaa
     4381 aagcatttta atttttacat tggtagcttt aattgtattt ggaaatattc ctagactatc
     4441 tattatggca gatatcaatg aaaaatatag acctacaaat gacaaaacgg tgtacgagga
     4501 cttaagtcaa tattttaaag gttttgatgg aagttttgtt atgtatgatt taaaatccaa
     4561 acaatatcat atttacaata aagaaaaaag taaaacaaga atttctccaa attctactta
     4621 taaaatttat agtgccttat ttggattgga aaatgggata ataacaagag ataattcaca
     4681 tatcccttgg gatggaaaac atcattctaa tagttcttgg aatgaagacc aagacttatt
     4741 ttctgctatg gaaaattccg taaactggta ctttcagact ctagataaaa aaacaaagct
     4801 aaaaaacata caaacttatc ttaagcaaat tggatatggc aatcatgatg tttcaggtgg
     4861 tatctcaaac ttttggctag aatcttcatt aaaaatatca cctattgaac aggtaaaatt
     4921 acttgaaagt ttttatacaa ataagtttaa atttgaagat aagaatatag aagctgtaaa
     4981 agattcattg ttaatatcaa aaaaggatgg tgtatcactt tttggtaaaa caggcactgc
     5041 caatataaat ggaaaagatg taaatggttg gtttataggt tatgtggaaa aaaatgaaaa
     5101 tacttatttc tttgctacaa atattcaaag caaagattat tctaatggta gttcagcatc
     5161 taagataaca ttatccatat taaatgacaa aaaaatctat aatccttaat atatttactg
     5221 tacattctaa aaaattcaca taattttcat attaattatc gattaaattc atatacaagg
     5281 cttattatat aaacatagaa gatagtaaaa ctagcttaaa aacataatat aataccctat
     5341 acaaatagtg gcaactctcc ctcacatcct caattgttgc cactatttaa ttttttgtat
     5401 gaagttaaat atattttcat catttcaaca ataaaaaaga ggtctatctc tattttgaaa
     5461 caacctcttt aattatttta gagtttacta agaagtctaa aaacaatgta tctaaaatat
     5521 tattctcaac tgagtaagat taatttccaa attgagtaaa tttaatcttc aaatactgat
     5581 ttaacaatag gaataacata atgacttccc ccacgactct ttacatcttc aatcatagca
     5641 attattagat attgtttatc actattttca tctacagtaa aagcattaaa ccatccaagt
     5701 tctgtccctg taacatcatc ttttgatgct ttaatttctg ctgtaccagt tttcccagca
     5761 atgctaattc ctttaatcat agcactatga cctgtaccat cagggctttc aacaacttga
     5821 agtaagtcat cacgaataat ttttacagcc tctttagaaa acactccttt cttccaaaat
     5881 tcagcctttt taaattcaag atggggtttt atcatgtctc catcatttac aaaagctgaa
     5941 tatattgatg ccgtatgtac tggatttaca agaagcttac cttgtccata gccagtatct
     6001 gctaattgta tttcagtctc aaatttattg tcagtgccaa attttgaaga agacaatcca
     6061 aattcaaatg gcatactttc atcaaatcca agcttcaaca actcttttgc taatacatct
     6121 tcacctattt ttaaggctgc cttagcaaaa taaatattgt cagaataaat caaagcattt
     6181 ttcatatttg cagtatttcc ataatctttt aacgtggtta ccttataact tccccaacta
     6241 gaatcttttt gccaacttag actgctgtgt ccaaagtttt catttggatt aatctttcct
     6301 gttgttatac caataccagc agtaactggt ttaaaagaag acccaggaca taatcgtgct
     6361 ttaaatctat tgtacattgg tttattttca tttttattga gttcattcca tctatcttct
     6421 gacattccca atataaactc atttggattg tacgacggac tacttacaag tgataaaacc
     6481 tctcctgttt taggattcat aactactgca caacctttat catttttcat ttggtcatat
     6541 agtttactct gtagttttga atctatagtt agctttacat cttctccatc tacctttggc
     6601 tcagttacta aagtttcttt cttgtcacca tatctatcag ctattataat ctcatatcca
     6661 tcaataccac gtattctatc ttccagtaaa ttttctaaac caatcttacc aattatacta
     6721 ttatcgttgt acctcttacc ctctttttcc tttagtatct ctgcatttac attttgaaca
     6781 taaccagtta agtgaccagt tttttcacca aatggatata ctctaacctc tgtattagat
     6841 attttaactc caggaatact aagaagtgac tcttgtagtt ccttatcttc ttctttcaca
     6901 gtaccatctg cattttcttt tattttttca atagttttta taggtaccat tgaattttct
     6961 tttacccaag atgcatttaa tttcttttca atttgttcaa ctgatacatt caataaagta
     7021 gataattttt ttaagtcttc tatattatca ctcatttttc caggaactaa tcctatcata
     7081 gatgcaagac cttgaccagc aatcattaca ccgttcctat ccagaatacg tcctctctta
     7141 cctttcagct ttgataccct tacagtatta tcttcttcaa gttctggaaa tatgacgttt
     7201 gactgccatt ttattttata atttttctgt ctatctctag ttaatagtac ttcatttgaa
     7261 aatgaaattt cacctgctaa agtatccatc ttagaatcat agtttattac tttatcatta
     7321 tttttatttt ttttaatatc attaatctta atacttatat cgtgtgcatc aattccttca
     7381 tagatttttt tatttcttaa aacaaaatct tcttttttat tttcagactt gctcttttca
     7441 tctaataaat catacatttt ctcatattgt tttttatttg caaaagacat atactgctta
     7501 aacacttcat ctggtttgtt tttattgagt ccatctagta tgaattttgc taaaaaaaca
     7561 ataataacta taagcaaaca cattagaaaa ccaaaaatta tcattttttt tgttttttta
     7621 ttttttctca tataaaccac ctttatcatt taatatatta catacgtaag ctttaaggtt
     7681 aaaatactct tatagacata gctaatctat gtttttatta tgtttttaag tatctcataa
     7741 aaatatgtaa gcagtataat tgatagtaat ttatatatta cacatgtaag ttttaaaagt
     7801 caataatatt atcattattt aattattttt tacatcaact taaaaataaa tcagttccat
     7861 tatattcttt tgtattcttc aactatttta taaattaacc ttattctatt gtaattattt
     7921 tatggtatat aactttaact tgacaaaacg acacaagtgt ctcataatta aaagtgacac
     7981 ttatgtcgtt aaaaaggagg ttaaaacata tgccaacaaa aggagaacaa actaaacaat
     8041 ttatcattga tacatcaata atgctatttt ctgaaaaagg atatagtaat gtgacaatga
     8101 aagatatatg tgaagcatgt aatttaagcc gtggtggcct atatagacac tttaattcac
     8161 caaaagaaat ttttattagt atattaaaca ctcataaata ctactctcaa aatttgttgg
     8221 ataaaggtat caaggaaaat atatcagcta agcatatgct agaatatttt ttccaacaac
     8281 aaaaggctga cataaatggc aaatattgtg gatttgattt tgcaatacac gaatttgctt
     8341 ttgtagaaaa agaccaacgt gattatatga ataaaagata caattcagct atcacaatgc
     8401 tttcaaattt aatcgattat ggacaatcta aaagtgaatt taaaaaatgt gattctaaat
     8461 cagttgcaat acacataatg ttcttatttg atagcatgaa aacatctgct tccattttaa
     8521 aacttacaaa taaagacatt gataatcaaa taaatattat aaaggacatg gtgattttat
     8581 atgaataaac aaaaattcca taaaattgat ttacaaaatt gggaacgata tacacatttt
     8641 tactactttt caaaaatgac aaatgtaggg tttaatttga ctatagaaat agatattact
     8701 aatttgctta aggaaacgaa aaaaagaaat attaaatttt atccagcgta tttatataca
     8761 ataactactt gcttaaatcg atatagagaa ctaaaaatcg catatgatga agatgaactc
     8821 ggatattgga acacgttaac gccactttat gcgatttttc atgatgataa taagactttc
     8881 tcatcaatat ggacagaata tgatgaaaat ttctcaatat tctatgaaaa ttatttagct
     8941 gatgttaaag aatatggaaa aaatcatggt tttctagcaa aaccaaatcc gccaagaaac
     9001 tgctatactg tttcctgcct tccatggatt agctttaaga gttttactgt acataatcat
     9061 ggtcttgaaa attatttttt ccctacaatt gaagcaggta aatactatca aaaagatgaa
     9121 aaaatacttc ttcctctttc tataacagtg catcacgcaa ctactgatgg ataccatgta
     9181 agttgtttta ttgattacat acaaaaaatg tgctttaata ctaagatatg gctacactaa
     9241 aaatttatac aatataaaaa gaactgtcgc atgagcaaat taaatttgct atgagaaagc
     9301 tcttttttac ttaccaatag ttaatatgag atgaattatt tcttatacct tgaacttacc
     9361 aatagttaat atgaaatgaa ttatttctca tactttgaaa atattgaatt gattttgata
     9421 atcttctttt ataatcatta cgtgtcacct actactcaac acatattctt tgacctattt
     9481 ttacatcctt gtttatatcc aaaatattta ccttcttacc atctggaaac aataccattg
     9541 tcaaagtaga ctttcctaat tcgtgtattc tctttaaatc aacattaaca agaacatccc
     9601 cctgtttaac catatctcct tgtttaacag caacatcaaa gccatctcca tttaattcaa
     9661 ctgtatcaat tccaacatga attaatactt ctccattagt tcctttaatc ccaaatgcat
     9721 gtccacttga aaatactgtt tctaatttac ctgaaatagg ggaaacaatt tttccatcta
     9781 taatatcaat agcaaaacca tcacctaaca tttttttaga aaaagtttca tcaggtacat
     9841 tttctaatgc aacaatctta cctttagctg gagacacaaa gttttcatta ttttcattaa
     9901 acactaattc ttcgtcttca tcttcattta tagcttcttt aggtactcca aataaacatg
     9961 ttgcaataaa gcagaataca actacaaata ttgatactaa taagtatgtg attaaattat
    10021 atccatcata gatatacatt aaataagaag gtaaaactgc taatccatat gaattagatt
    10081 gaatatgtaa aattgataat attgctgctc ctaaacctga agatgcacac ataataatca
    10141 aaggctttaa attatatcga agctgaatcc cgaataaaac aggttcactt ataccaaaaa
    10201 gttgagatag cattgctcca actgcagttt gtttaacttg attattttta gattttttca
    10261 taaatgctag acatgttcca atattcgcaa atccatacat tgcacaaaga gtaataagtg
    10321 cattaaatcc tgtatttgct aatagtgatg tttcaatcat tacataagta tgatgtaagc
    10381 cagtaataac agctaatgga tatgtaaatc caataataaa tcctccaatt cctaaaggta
    10441 aaccaatcaa taagctgatg atatcaacca tttttaattc tattgtatgc attataggcc
    10501 ctacacctaa caaaataact aacattgtta ttagcataac aaagaatggt gtgaaaataa
    10561 ggtctaatac attaggcata tgtttacgga accatttttc aagattagct ccaataaatg
    10621 ctgtaacaat agctgttaaa acacttcctt gacagcctac aataggaata aatccaaatg
    10681 ccataattgc ttcaacttca tttgatgggt cagcaacaga atatgcatta ggaagtattg
    10741 gggaaactaa cataagtcca ataacaagtc caataacagg tgttcctcca aataccttaa
    10801 atgctgacca tacaataatt gctggtaaaa aagcaaatgc tgtttctgta agaaccgaaa
    10861 caagtgtctg aatatacaat ggaatatttg cagaagacat tccaaataat ccaagtacat
    10921 tatcattaaa cagacaaccc tttaatccca agaataatcc tgttgccgca ataactggaa
    10981 taataggaac aaatatatct gctagagttc tcataacttt cttaaatcca gtttcattat
    11041 ttttaactaa ttcatcttgt tctttttttg ataaagtttt caatcccatt ttttcaactt
    11101 ctgcataaac tttattgact attcccgttc ctaaaataat ttgatactgt ccagaagtaa
    11161 agaaaacacc tttaacctca ttaacctttt ccactttctt atcattaata atttctctat
    11221 ctttaaccat cacccttaat cttgtggcac aatgtgtaat agaaataata ttattttcac
    11281 caataatttc aataagctca tttgctattt tattatattt attcattttt ctacctcact
    11341 ttcaataata ctgcttgaaa tggtttcaaa ctcactgttt tatttagtag atttatatca
    11401 tctaaatttt ggaatacaat atcatatttt tcttctgttt ttatttctac aggtttatca
    11461 gaaaaattat aataacaatc aataactacc aaatctaatt gccttctata cgcaataaca
    11521 ttgtcacttt ctatatcaat aggttctatt gagccataaa tcaaacaatc agaatactca
    11581 ctatcttgtc tcaactttat catcttttta taaaattcta aaatactttc tgaattgtta
    11641 atctgactct gtgcattaat atctttataa ttttcaataa cctttaacca tggtgttgta
    11701 tctgaaaaac ctgcattttc tttatctgac catggaaatg gtgttcttgc attatctcga
    11761 cttcttaaat tcacaaattc taacgcttct tcttctgagt aaccttcatc tattgaacga
    11821 tagtattggt caatgctaga taaatcatta aaatcattta tggtatttct tttaaaattt
    11881 gtcattccta attcctgtcc ctgataaata aatggtgttc ctcttaaaaa gaaatacatt
    11941 gctcccaaca tttttactgc atcatcattc ctttcgtatt gcaaaagata ctttgttgta
    12001 gctcttggtt ggtcatgatt ttcaataaaa ttagctgccc atccatgttg ctgaacatac
    12061 atttgactat ctaaaatgta ttttcttaaa tctgaaattg tccatgaaat tcttttaaac
    12121 cactcactac cagaagcaac atctaagtca gcatgtttaa agtcaaatac cattgaaaaa
    12181 aaaccatctt ctccaataaa ttcacctagc tggtcatagc taatacctgc tgcttcagca
    12241 acagtcatac aatcatattt tttaaacgta ttttctttta attcataaag cattttttct
    12301 attccaggct gatttctaac tgcttttgta catttaacaa gaccatcaac accatcactt
    12361 tctaaacttt gaaaacttaa attctttttg atgaatgtaa tagcatcaac tctaaatcct
    12421 gccactccca tttgagacca ggtattaatc atttggtaaa gttcacttct taatattgga
    12481 ttttcccaat taaggtcagg ttgttttttt ccaaaagaat gaaaataata ttcgtctcta
    12541 ttctctacct tttcccaaac acttccgcca aaaacagacc tccagttatt tggcaaatcg
    12601 tttccttttt taaaaatata ataatcatga taaggacttt ctgggttttt taatgcttct
    12661 tgaaaccaag gatgttcatc actcgtatga ttaagaacta aatctaatat aatcttaatt
    12721 cctctatctt tactttcttt aattaactct tttaaatctt ccatacttcc aaattcactt
    12781 gcaatatcca aataatcact aatatcatac ccattatcgt ccataggtga tttaaaaatt
    12841 ggacataacc acaatgatgt aatacctaaa tcttttaaat aatctaaacg agaaacaatt
    12901 ccctgtaaat ctccaattcc atcattatta ctatcttgaa agcttcttgg ataaatttga
    12961 taaacgattt ctttttgcca ccatttcttt tccataaatt actccttttt atatgatatt
    13021 tcttcttttt aattctttat aaattccatt atcgaatatg tcctcacata tagatgttgc
    13081 tatatcttta agctgttgat gactattttt cattgcaatt gtaatatctg atgcttgaaa
    13141 catgacgata tcattttgac catcaccaaa acatatggtt tctttttgag ttatccctaa
    13201 tctttcttgt aatctttgaa ttgctttgcc cttatgaaaa tctttttgaa taatttcata
    13261 atactgacta gaaatatccc tctgcgctag ttccatttta tcttgtagta tatctttcac
    13321 ctcatcaaac accttttcat ttgaccataa acaaatttta tgtatatctt gtgacttata
    13381 ctcttcaatg ttattttcat atgttatttt ttcttggata tgttgtttat ttatacaaga
    13441 attagttcct ttcaactgat tcattgtttc aaatatttct ttggcttttt gattcatgaa
    13501 aactttttcc cgactctcaa tcgaaaaagc aacttctctc ttttttaata aacaaacaac
    13561 tttctttatc aatctttgat taaaagattg attataaagt aattcaccat gatattgaat
    13621 gtaattacct cctcctgcaa tatatccatc aacccctaat gataaaacat catcttgtat
    13681 tgtacccata cttcttccag tacaaataac aacactgcaa tgattctttt gacataaacg
    13741 tatagcatgc tttgcagatt ctgggattcc atctacctca tctctaagcg taccatctat
    13801 atcaagtatt aataatttgt acaatttcat cttcctttct tactatcaaa atcataatag
    13861 cattcaaaaa aaaagatatc aatattttga tatcttttaa aaaatatgtt catttttagc
    13921 acaaattttc attttcgggg aatatgttca ctttctaaat acatcttgta tttaatataa
    13981 agcagttcaa ttaaaataaa ataaggtgtt gttacatcgt agtccagttg tttatctaca
    14041 gaaatagata tagattgaat atataggttt tcatcacata atcttgctaa aggattactt
    14101 atcatttttg taatagaaac catataaatt cctttcattt tcattttttt agcaaagtca
    14161 ataattgtag atgcatctcc atttaatgaa ataaagaaaa tcatatcatc cgtttttata
    14221 aaatgattaa gtgcctgaat catatcatag ccataaacat catatataat tttccctgtt
    14281 ggtaagaaaa tccttttcat ctctttagca actctttcct gagaataacc ttctccaaag
    14341 acgattatac gatttgcctt tttcattctt tcaaacataa tcgaacaatc cttcttctct
    14401 atatcatcta taaccttatt ataacaatcc attaattggt gtttactttg atttttatat
    14461 tctacttctg tttcatttaa gcgtataata actcttaatt cactatatcc aggtaattgt
    14521 agtttttgag caaaacgaga aagtgcactt gtagatatat ggtatttgtc tgcaaattca
    14581 tctatagaaa gtttaataca atcttcttta tgattaagaa tacactcaca gatatactta
    14641 tcatttgctg taaatgaatt ataatattta ttgaaaagcc cttcaatttt cattcttttc
    14701 tccttctata ctctatgtaa ttcatatgaa gcaaatctaa tagagcataa aatgctccta
    14761 cgacttcata agatatagct tgtaatgtat caagtttttg cgtttcaaca tacagattat
    14821 actcacaaag tgttgcaatt gtattattat caagcctagt taaagaaatc gtatgtatat
    14881 gattgtttat aaattttgca atttcaattc ctgctttcgt ttctccggac aaagaaatga
    14941 taataaacaa atcacttttt tgaaatgatt ctctcataaa ttcaatttca cctatatcaa
    15001 ataagtcaat aacacattta cctactgaaa gaaatattct ctttatttcc tgagctaaac
    15061 ttttctgttc gttacctgtc ccataaatat aaattgtttc tgcatcatat attattttac
    15121 aaatatcttg aaatgatatt ttttgtaatt cattcattaa tagtcgataa ttctcgcaaa
    15181 catcatgaaa attattatca ttattaacaa attctagttg attattttta agtaaatatt
    15241 ttaaatcaga gaatgaatct aatccaattt ttctacaaag ccttaataaa gtagctctag
    15301 aaacattaca ttcatttgca attttttcac acgaaaaatt acggtattct ctttgacaaa
    15361 tcaatatttg tttaataata attttatcat tttcatttaa attatcataa ttttggttaa
    15421 ctaacgattc taatctcatt ttttccactc cctaaatatt attttaatat acattagttg
    15481 gtttttctag ggaagagtct taaaaatatt tctattaaga caaataaaaa agagctatat
    15541 cagaataaat ttaatttgct cataccacaa ttctattata aataaaaaat ttttcacatt
    15601 attttttaca ctatataatt tctattttta taattaaacc caatttataa gatatagaac
    15661 catatttata tttttatatg ctgatttgtt ttaattacat ctattcattt tctatcttat
    15721 ttaataattc ctattacttg aatatttact tctttgaata aatttcatag ctaacaaagc
    15781 taaaattatt attatagatg tcaaaataca aatataattc atattaaaaa agcttccatc
    15841 tctttcttct atataagtca caaaactaat aaaccattgt gttggactta taatactaat
    15901 tttttgtaac attgttggca ttttttcaac tgaaataaat gaaccaccaa taagtgataa
    15961 tataagcgat atacatgtag aaaacaaatt tgcatgcatt tctttattga ataaagaaga
    16021 tacaaatgtt ccaaaagaag ttgccaaagc cgatatccat actcccatta aaattaaatc
    16081 tatataatcc ataccaatat caatattaaa taacttaatt atagatattg aaactatgta
    16141 ctgaaaatac gttattgtaa agttgtataa catattacct agcacatatc ctcttatatt
    16201 ttgtggagaa tacagcactc ttgtaaatgt acctgagttt ttatctttaa taatcattga
    16261 tgctgttaca gtagatgtta tcattaaaaa taatacgata aatgcaatta ttctttgtaa
    16321 aactcctaaa gtattttctt gtaatttgct tatatctaca ggaactgggt cttttttata
    16381 tgtttctatt aaatacttta atgtatcatt cgtattttta tcttttatag aatataactt
    16441 aaaaccattc ttattaaaac atatgacagc tgaatacttg cctgttataa tatcagtttt
    16501 aattgtttta aaatctgcaa tctctgtatt aatcccttga gtattttcta aagccattat
    16561 tgtattttca taggtccttt tttcagattc ctttagaact cgttcgtttt tgtactcatc
    16621 taaaatacca atattaaaag atggtttact tatgtaattt gccaatactc ctaaaattac
    16681 tataattata ggaaataaaa gtgtaactaa aaacactaat cgcttattta tatttctttt
    16741 taagttacta ttaaaaacat ttattacatt cactatataa agacctcctt tttaaaaaat
    16801 ttgattgtaa gtattgtaat aaatatgctt acagctacaa atatgcatga tatattaaaa
    16861 aggagttgta aattattatc atagatacaa gcaactatac ctctattaat ccatgtcaat
    16921 ggtgatatat taatgattaa tgaaataact ggattcaatg agctaactgg aaagaacact
    16981 cctcctaaga aaccaaaaat aactattggg atattaatga tattacgtaa catatctaaa
    17041 tttttacaaa caagacccaa aaacaaaccc atagatacta ctgcaataga ttcacacatt
    17101 aaaaagaaaa taacaactaa aaatttccca ccaaaactca tatgaaaaat tcctcttaac
    17161 accgctaaag ttatcaaatt gcatattgaa agtactgctg tacaagaaaa aagttttgaa
    17221 ataactaaat ctactattga aataggagaa atcatataac gatatgatgt gttagaaatt
    17281 ttttcatctt gtgccacata tgaaacacta ataattccca taagaacaca aaatggtatt
    17341 gtaacaatag tataataatt atatgatgta aattcctcac cataggattt tgatgtcaaa
    17401 tatcctaata gcattatgat tattactgga aaaacaatat tgtaaaacag aataaaaaaa
    17461 tctttacttc ttcgttttat tcctaattta aacaatggta aaaaatatct catctattta
    17521 ttcctccaaa tctctcaaat ctgtacctgt aagatataga aaaatatctt ctaatgtctt
    17581 acaaccaaat tctaaatatt tatttttata taaactcttg tctaaatctt ctataattac
    17641 tcctttatct attaaaacaa ttctattaca taaggtctca atttcttcca tatagtgact
    17701 agtatatata actgttgtac cattagcatt aatgtcttta attgccttca aaattttatt
    17761 tcttgattga gggtcgatac ctactgttgg ctcatccatg atgagaatct ttggactatg
    17821 cgcaattgca caagctatgt ttagtctacg tttcattcca ccagaaaatt ttaatggaaa
    17881 ctccttattc ctctcccaaa gaccaacaaa ttccaaagca tctttagttc tccttttaag
    17941 ttcattccct ttaaatccat ataatgagca aaaaaattta acattatcat acgcacttaa
    18001 atcttcatat attgctaatt cttgtggtac aactccaagt gagcttttaa aaatattttg
    18061 ttgattctta atatcttttc ctttatagta tatgcttcca ctatctggag acacaatcgt
    18121 agtaagcata ttgatagatg tgcttttccc tgctccattt ggtccaataa aacctaaaat
    18181 atctccttcc aataacttaa aactttctct tgatacaact tccttattgt tatacttctt
    18241 agataaattt ttaatctcta acaatacatt actcatttat aaaaccctcc taatatttaa
    18301 ccaacattat atatccaatg ttgtatatat agttttttta aaacacgagt attttactcg
    18361 tgttattgta ataaaattta ttacttattt tgtaatttca atattatctt atctatattt
    18421 tctatagctt tatctgggtt attaattatt tcatctctaa gtctctgaac ttctaataac
    18481 tcatttgtcg tatcagattt aaataaaaaa tcttcttcta tatttgaaaa gtctatagat
    18541 ttaattatat tctgtgtctc gcaaccaaca gatatatact tatctatatt atttaatatt
    18601 tcttcatgcc acaaaatttg ataattcaga ttatctatag tcatatcaaa tgcaattttt
    18661 tctgttgcaa gactcttttt atcaattttt ttaatctctt tccaattttc ccaatacttc
    18721 ttctgctcta taagatattt tatatgtttt tcaagatttt tttgcaaagt atccttaggt
    18781 aatacatcca aaatattatg taataggaat ttatttgaac cggttggaac tattggcatc
    18841 tgaagctctt tttgtatttc ttcccttaat tctaatttac cactttgagt aatctcatag
    18901 attttacgaa tcctagctcc tgttttttct tctcttaaaa ctctaactcc tccatctttt
    18961 tctaattttg ccaatgcata atatattgac ccagattgta ttttagtcca tctatctgca
    19021 ccagatactt gtaaaaactt ttgaatctcg tacccatgtg ttggcttaat atttagataa
    19081 tataaaatta aagtgcgtat cataaaaatt caccacctat attctatctt ggatattata
    19141 ttatcaccaa tattggatat agtcaataga taacatagga tatattacaa cttattattt
    19201 ttattattta atacaaggag ttccatgaga catacatatc cattcatatt ttaaattatc
    19261 caattctctt atagatttca tcatcttttt ataatcccaa ttccatggat tagggtaagg
    19321 aactatattt ccattcttat ttttgactaa atcacctgca aataatacat cttcaaaaat
    19381 catgcagaca tgccctggag tatgaccagg agtatggata atttttatat tcccaatctg
    19441 catattctct ccataaggtt caacatcctt aatgagcctt ctggaaattg cctttccaat
    19501 aaacttctta aacccaggtc tatccttctc tcctataata aaaggtatat cctctacatg
    19561 agcccatacc tttgctcctg ttttctcttt caataattta atattagata tatggtcaac
    19621 atcatgatga gttaataaaa tatgcttaat atctgtaagt ttaatgccaa gtgtttttaa
    19681 ttctttaatt atatttttcc ccataaaagg aagacctgtg tctataagaa taatttcttt
    19741 atcgaacaca ataaaacaat ttgaaccacg agtactatct aacattaata cattttcttt
    19801 tatcttcata aataaattcc ccttatttgg tattttacct atataattaa ttaataaagt
    19861 tataaatagt tataatactt gatattaatt ttatttttta ttaaagtata ctataggtat
    19921 acaaatatta tatttacata agtataccat cagtatactt ttttatcaat agaaaggaat
    19981 aatttatatg tcagaattat caaaaagaga taaagaaaaa atacaaagag aaaatgagat
    20041 tattgataag gcagaaaaat tattttgtct taatggattt gatagtacaa ctatgagtga
    20101 gttggcaaag gaagttgaat atactaaacg taccatatac aagtactttt catgtaaaga
    20161 agatttattc tttgctgttg tcttaagagg atataaaaga ctttgggata atgttaaaat
    20221 agaaagtgca aaaggcaaaa cagggtttga aaaaataaaa ctttcatact ttgcttttca
    20281 taaattttac tctgtagaac cttctctatt atgccttatg ggaatgattg gtattgtaaa
    20341 ctctgaaaaa tcggatactg atatgctgtt taaagaaaag tttttttctt ttaataaatt
    20401 tatgtttgat gaaatacaag aaatgtttga aattggaaaa aatgatagaa gtattaggca
    20461 tgatattgaa atacctactc ttatgtattc atcaatattt acacttacag gattttttaa
    20521 tttactatct gtaacaggaa aatcatacct gaacaacttt aatatagatg aggaaagatt
    20581 tattgaaaca actcttgctc ttttaataga ttcaattaaa gcttaaacct aagtttataa
    20641 attaaagttt atatacttaa atgataattt tttctatctc taacttctat ctctaacata
    20701 aaaaatatta aacataaaac taataactta gttaatgaaa taactaaatt actgaaagga
    20761 ctaaaagtaa atggctaaaa tacttctttt ggaagatgat gatacaatta catttggaat
    20821 aaaaacttcc cttgaaaaga aaaatcacac tgtatgttgt tataatacga ttactgaagc
    20881 taaaaaaaat tttgataaaa ctattgattt aattctgctt gatttaaatc tacctgatgg
    20941 tattggatac gagttttgcc aatatgttaa aagtatagaa gatactccag tgatattttt
    21001 gacagtccgt gatgatgaaa aagacattat acgtggatta gatatgggtg cagatgacta
    21061 tgtggttaaa ccatttttgc tttcagtact tcaatccaga attaatgcag ttcttagaag
    21121 aactcattcc aatactaaca atactattac atgtggaaat ataagcttaa ataaagttga
    21181 agtcaaagca tatttagata atgttgaagt acctttgaca gcaggagaat ataagcttct
    21241 tctaagtctt atggaaaata aaaatagagc tatgactcgt acaaagttac tagaaatgct
    21301 ctgggatgta gatggtaatt tcgtaaatga caacactctt actgttacaa taaagcgtct
    21361 tagagaaaaa ttaaaaaata caccttgtat aaaaacatta agaggcatag gatatagaat
    21421 ggaggacagc tagtgaaaac tccaagaaat atcaacagta cagttgtttt tataatattc
    21481 tgcttaattg taactttttt attgagtgta attctaacaa ctataacttc ttttaatgaa
    21541 tacaatgtac tgtctaaggc tcttggtgca tcatatgagt acaatcctaa gtctacagaa
    21601 agtatactaa aatcattgaa aaattctagt gatgaggatt ttaaaacagg aaatgagatt
    21661 ttatcaaaac atggatattc taaaagtaca ttttggagta aaaacttttt ttattttcta
    21721 gtggtatcgt ttggtataat ttcaacaatg actatagttt tatatataat caaacataga
    21781 acaaataagc aaaaattgag tagaataaac aatcttactc aatacttaga agaagtgaat
    21841 ttaggaaaag agtctgtact tttacgatgt gaagatgaat tttctcacct tgaagatgag
    21901 atatataaaa cagttgggga acttcgttgt tcaaaagaat ttgctttaaa agaaagacaa
    21961 actctttcag ataaccttgc agatattgct catcaactta agactccaat tacttctatg
    22021 tcacttatgg cacaattact ttcagaaaat tgtccagatg aagaaataga ttatattaac
    22081 aggcttacaa atcaaatctc aagacttgag agactagttt catctttact tacattatca
    22141 aaattggata caagtacttt aatatttgaa agcaaactta tagatgtaca ttctcttctt
    22201 acttatgcaa ttgagcctat agaaggttct ttaagagaaa aacaccagac atttactatc
    22261 gaaggttctc cttcggtaga atttgaaatc gatattaatt ggactttaga agctctttta
    22321 aatattctta aaaattgtag tgagcatata gaagatggtg gatatatagt tgcttattat
    22381 agtcaaaatc ctctatatgt tgagattatc atagaagata atggtaatgg gtttgctcag
    22441 gaagaacttc catatatttt taatagattt tatcgtggta aaaatgctag taaggacagt
    22501 attggtatag gacttgctct atcaaagtct attatagaac gtcaaaatgg tactctacat
    22561 gcagaaaatt gccataatgg tggtgcaaga ttcataatta aattttacac ataaaatgct
    22621 ggattatagg taaaatacct ataatccagc catatctata ttctatgtta tctattattt
    22681 tagtaatata cttctattta catttaagat tttattaaat aaacaactta actatcccta
    22741 tacttgtaag tagtcttaaa tcgaaaccct taaataagtc caaatagctt ttgtttatat
    22801 tgtttcattt tttatagatt ctactatatt ttcattcttc aactgcttgt atcctgtcat
    22861 atatgatgct attacaataa aaagtatcaa tatggtaaaa cctattattg tagatacagg
    22921 tgcaaatggt aaatactcta taaaatatat ttcatttata cgtagaaaag tatacactat
    22981 tcctatttgc acaggaatac taagtagtaa tggtgtaatt cctaagaaca aaccttccaa
    23041 aacaagcatc ttttttatgc catctggaga taatccaaca gaacgcaaca tagcaaactc
    23101 ttgtcgtcta cttctaatat ttcctgacac tgtagcaagt acatttgata atccaatcat
    23161 agccaataaa cctgtaataa agcctactat caaattcatg gtctttcttc cactagcatc
    23221 ttgtttttca ctttctaaaa catcctcaat actatagtct cccgaaccat agtctttatt
    23281 acaaattgac tctaattttt ctcttactct agaaatgtcc ttacgattat ttactttaaa
    23341 taatcctata agcctatttg aacgtaacct acttacttca tcataatttt tagatatttc
    23401 ctgcaccata gacattggca ctatttgcat taatgtatat cttgaactag tatctactat
    23461 tttaggaagt gtgcttgtaa tagctcccac tttcatgttg taagtataat cccctttatc
    23521 ttcgtcatat accttttcat ttaatttaat gttatctcct attgaaatac ttaaataatc
    23581 tatatacact ttatttctac gtgtgctacg attaacatct tcctccttat tatatacaat
    23641 tgaggttgga ttttttgtat cataaaataa ttttgggtct acagaaattt gtctgcaata
    23701 ttctccaaaa ctcttatcat caagcccaat tatatttgaa attatacgaa atttgccatc
    23761 acgttctata ggagagtatt ttttagatga aactatactt ttaaatcctc catttttagc
    23821 aagttctttt gaagcatctt tttcagtcag ccaagtggca gcagacattt tttgataata
    23881 tagtgagctc tttacacctt ctatattatc taatttttca ataaaagtag actctacagg
    23941 ttcactattt tccaaataaa ctgctatatc tctttggtct ttataattat cacttccata
    24001 aacagctttt gctccttctt gattggcaat tatatgcaaa aaacctgtaa gtaataaaaa
    24061 agataatgtt aaagatattg ttgcagtacg ataagatttt ttacgagcac gtagtgcatt
    24121 tgatgcaagt tctccttcta ttccaaatat ctttcctaat gaatatttat gtgacttttt
    24181 gattttaaca ctatctcctt gtcgtattgc ctctatagga gatatttttg atacttttcg
    24241 tgctggtata agtgcagaaa tccagactgt tactattgta agtaaaactg ctggcagaaa
    24301 agcaggaata ccataagtaa ataccacttc tggagcatca gtatgataat taactgaatt
    24361 aatatagact ataagcctat tacacaatag ccatcccaaa aataaaccta ttggtaatgg
    24421 tataattgtc agtaaaaagc cttcaaacac tacagacctt ttaatttgtt ttggtgatgc
    24481 accaacacta gataatatcc ctaattgagt aagtctagca tttgcagaca gtgaaaatgc
    24541 attgtaaatc accattacaa acacggctac tgtaaaaatt gctattgtaa gatacataat
    24601 aggcgttgaa aattttgata tactactcat agatgctttc tgttctgggg aaaagactaa
    24661 catctttaaa agataattac tattgtattt aagattgtat tttccatatt cattagtctt
    24721 ccaccccaat gatttagcaa gttttggaag ttccttatac gtatctctta tatttttaaa
    24781 tcgcaaatac acagttatac tatcattagg ttttatactt gttttatcaa gatagccaag
    24841 agctgtatat gcaggtatta ctgatgaagt agaaacatct acctttccta ctattgtcaa
    24901 tgtagtctct gaaatttttt taaaactttc ccctttcgat tttggggcta tgggttcaag
    24961 aggctttcca tttaggattc tatctcctat tgggagagta attttatcac caatttttac
    25021 acttggatta ttctcaaaat attgttttga tagtgcaatt tcattagtat tacttggtcg
    25081 acgaccctct attatagtgt ctttttctgg catatgattc caatactctg atgtagcatt
    25141 tctataaatt atataatctc ttttgtcatt attaattttg cctacatacc attgtccttt
    25201 taccattaca tcatcaactg aagaaaaatt ttcaattaac gataagtctt ttccataagt
    25261 atcgtcaaac aattcaccat gccagttacc ttctttctca attgaaagtc ttataaaatc
    25321 agtccacata gtatacaaca gtccacacaa ggaagacatc attgttgtag taagtaatat
    25381 agccaacatt atagctatac tactacgttt attgcgtcgt acaaaatcta aggtgtattc
    25441 ttgaagaata ttcatttctt caccaccttt tcagagacaa tcactccatc ttccatagtt
    25501 acaatacgat ttgcctctag tgctattttc tcatcatgag taataagaag tattgtctga
    25561 ttaaatcttt tatttgataa gtttaacaaa tctacaattt cttctgaatt ttttctatcg
    25621 agattaccag ttggctcatc tgctaataaa atagctggtc tatagattaa acttctagct
    25681 atagataccc tttgttgttg tccacctgaa agttgacttg gaagatggtt taatctatca
    25741 gataatccca atatagatac aatctcagag aatctatcct catccacctt tcttttatca
    25801 agtaacatgg gtaataatat attttttcta acatcaagag taggtataag attataaaat
    25861 tgataaataa gacctacttt tctacgtcta aaaactgcaa gagcttcttc ctttaaactt
    25921 gatatttctg tatcttcaac ataaatcttt ccagatgtag gtctatctac tcccccaagc
    25981 atatgtagca atgtagattt accagaacca gatgctccta caatggatac aaaatcacct
    26041 ttttgaactg ataaatttac attattaagg gctgtcactc tggtttgatt tgaaccatat
    26101 atttttgtga gattttcaca ttttaatatt tccatataca atcatctcct ttatggttaa
    26161 attttagcaa cctaaaatga cattatagtg acataaatat aaatagcgat tactattaaa
    26221 aatattgttt agtttttgca cattatttaa aattataata taaaacaaaa acta
//
LOCUS       DAEMAE010000034        25807 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEMAE010000034 DAEMAE010000000
VERSION     DAEMAE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.034"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            204..1397
                     /locus_tag="KPH93_001136"
     CDS             204..1397
                     /locus_tag="KPH93_001136"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG1687967.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1603..2721
                     /locus_tag="KPH93_001137"
     CDS             1603..2721
                     /locus_tag="KPH93_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG1687968.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFNPEVYEKMKLRVRELARGFGLAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2852..3097
                     /locus_tag="KPH93_001138"
     CDS             2852..3097
                     /locus_tag="KPH93_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG1687969.1"
                     /translation="MKEKIQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3084..3830
                     /locus_tag="KPH93_001139"
     CDS             3084..3830
                     /locus_tag="KPH93_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1687970.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKEGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLKK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4114..4989
                     /gene="hslO"
                     /locus_tag="KPH93_001140"
     CDS             4114..4989
                     /gene="hslO"
                     /locus_tag="KPH93_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG1687971.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5380..6180
                     /locus_tag="KPH93_001141"
     CDS             5380..6180
                     /locus_tag="KPH93_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG1687972.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEYKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKRERKIKDDLYFIKPKYVKYIPRNERAIYKALDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6706..8946
                     /locus_tag="KPH93_001142"
     CDS             6706..8946
                     /locus_tag="KPH93_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1687973.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELVEVIISIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSRFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPNAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIG
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIEMGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9346..10881
                     /locus_tag="KPH93_001143"
     CDS             9346..10881
                     /locus_tag="KPH93_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG1687974.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTVMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVTIFTSYLLNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFNKLQSEYKTKLK
                     MQWAGMYGFSNSYALAVRKEVADKYNLKNISDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRSEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10897..11658
                     /locus_tag="KPH93_001144"
     CDS             10897..11658
                     /locus_tag="KPH93_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687975.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQIDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKDKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDEDLKE"
     gene            11999..12868
                     /locus_tag="KPH93_001145"
     CDS             11999..12868
                     /locus_tag="KPH93_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1687976.1"
                     /translation="MDNNLITIEKTLNYIEKHLLDEVLDLDVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRKHQDFLPLQLKFDIHNRKKLRGDMILDVKKVNCADINIVGYSGKVKKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13190..13441
                     /locus_tag="KPH93_001146"
     CDS             13190..13441
                     /locus_tag="KPH93_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG1687977.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNANSYTIITAHKEKK"
     gene            complement(13754..14404)
                     /locus_tag="KPH93_001147"
     CDS             complement(13754..14404)
                     /locus_tag="KPH93_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1687978.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQFIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEECEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWIQGIGIGAGEMFGSLESVPLGKGQKKDLGVALKE
                     LSKNILSDNIHINDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDMLKK"
     gene            complement(14401..14904)
                     /locus_tag="KPH93_001148"
     CDS             complement(14401..14904)
                     /locus_tag="KPH93_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1687979.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDNNIKILPNTKEIHKCIGCFGCWI
                     KTPTECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(14953..15468)
                     /locus_tag="KPH93_001149"
     CDS             complement(14953..15468)
                     /locus_tag="KPH93_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1687980.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFIEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            15748..16275
                     /locus_tag="KPH93_001150"
     CDS             15748..16275
                     /locus_tag="KPH93_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1687981.1"
                     /translation="MHEQIDYCERKKIKTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFFAIDM
                     NEEAKLEAKENGRKI"
     gene            16259..17662
                     /locus_tag="KPH93_001151"
     CDS             16259..17662
                     /locus_tag="KPH93_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1687982.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIIVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(17909..18220)
                     /locus_tag="KPH93_001152"
     CDS             complement(17909..18220)
                     /locus_tag="KPH93_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687983.1"
                     /translation="MKKIIKSFTFCFFLIALFEIYMHQIGQDSKSIILIGLNPILSII
                     STIDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIRNKT
                     K"
     gene            complement(18276..18869)
                     /locus_tag="KPH93_001153"
     CDS             complement(18276..18869)
                     /locus_tag="KPH93_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1687984.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLENLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            19709..20359
                     /locus_tag="KPH93_001154"
     CDS             19709..20359
                     /locus_tag="KPH93_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG1687985.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            20550..21221
                     /locus_tag="KPH93_001155"
     CDS             20550..21221
                     /locus_tag="KPH93_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687986.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            21218..22348
                     /locus_tag="KPH93_001156"
     CDS             21218..22348
                     /locus_tag="KPH93_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1687987.1"
                     /translation="MNSLRKIVEKLKNVDISNWIKPNFGLYSVRKKVFLLSKLSGALI
                     ILFYLIVEEFSLNSKFEFLIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQKALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYDGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILAMQGLEYGVKNRDKGVEFYFMIPIDE"
     gene            22790..23635
                     /locus_tag="KPH93_001157"
     CDS             22790..23635
                     /locus_tag="KPH93_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687988.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFTNKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMAIDKPKNL
                     KMKNSNNKVEVEFLYNGNRDIEIVDIQELESGITFKYDKILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            23635..24663
                     /locus_tag="KPH93_001158"
     CDS             23635..24663
                     /locus_tag="KPH93_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687989.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLIHQVSSIDELKAKLDEDTNAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKEMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNVGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSRLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            24666..25679
                     /locus_tag="KPH93_001159"
     CDS             24666..25679
                     /locus_tag="KPH93_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1687990.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEILPQSRSKYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVAWFMLYIILDKKRKLS
                     FSR"
BASE COUNT         9781 a         2722 c         4533 g         8771 t
ORIGIN      
        1 tattatatta atattagaaa aattttaaat aaatgtttta ttctcaaaat atgcatatta
       61 atttaataaa atataccaaa atggaaataa aaaataaatt ttatgagaat ttaataacta
      121 ataattaata tatttaggtt ataataaata tgtgcgtaaa atctaacaat aagcaataat
      181 accgtaaatt taggaggaat aatatggata ctttaaagga actttttaaa ataggaagtg
      241 gaccttcaag ttctcatact atgggaccac aaagagcagc agagagattt aagaatgaaa
      301 acccagatgc agaaagtttt agagcaatat tatatggtag tttggcagct acagggaaag
      361 gtcatttaac tgactatata attgaaaaaa ctatagcacc taaaaaagtt gaaattgttt
      421 gggaagaaga tattataaaa gattttcacc caaacgggat gaagtttgaa gctttagata
      481 aagataaaaa tgtaacagct gaatggactg tatattctgt aggtggaggg accatagcag
      541 aagaaggtca aagaaacagt aaaagcaata gtatatatca cttagatact atggatgaga
      601 tagtaaaatg gtgcaaagaa aataataaga ctttggtaga ttttgtgttg gaatgtgaac
      661 caaaagacat aaaagattat ataaaaacta taaaagatgc tatgagaaaa tctattgatg
      721 atggactaag tacagacgaa ataataccag gcaagttatt attaaaaaga agagcaagta
      781 cattttataa tgcatataaa aaagataaaa gcttctcaac acttgtatat gcatatgcac
      841 tagcagcttc agagcaaaac gcttctggta atataatagt tacagcacca acttgcggtt
      901 ctgctggagt aataccagga atattctttg caatgcagga tttctataat tatgatgacg
      961 aaaaaataat tgaagcacta cttgtagctg gaataatagg taatataata aaaactaacg
     1021 cttcaatatc tggagcagag gtagggtgtc aaggtgaagt tggagcagct tgttctatgg
     1081 cagcagcagc agtagcatac ttaaaaggtg gaactataga ccatattgaa tatgcggcag
     1141 aaatagcact tgaacatcat ttaggaatga cttgtgaccc tgtatatgga tatgttcaaa
     1201 ttccatgtat agaaagaaat gcaatggcag ctcaaagagc ttatgatgca gctaattatg
     1261 cattattaac tgatggctca cattctgtct ccttagacca agttgttgaa acaatgaaag
     1321 aaactggtat agatatgatg gataagtaca aagaaacagc taagggtgga cttgctaaac
     1381 atttcttctc ttgctaaaaa taaaattttc caataataat tcctctgttt tgatttatga
     1441 aacaggggaa ttattattaa tggtgaatta tttaataatg caaaatattt ttatattcaa
     1501 tttagctttt tatttttaat tagtataaca tgttatataa tattttaatt ataaagaaat
     1561 atagaaatat tttttaataa attagaaatt ggaggagatt acatgagaga ggcatcatat
     1621 ttacaagaac aactttataa acataggaag tcattaaatc ctattgcaga gattgggaga
     1681 aaagagtata agacttcaaa atatataaga gattaccttg ataaattagg aattgagtat
     1741 gaagtttatt tagaaacagg catagtaggt gtaatagaag gtaaaagacc taaaaaagat
     1801 attgctttta gagctgacat agatggtctt catagtgaag aaggggtaaa acatttatgt
     1861 ggtcatgatg gacatgctac tatattactt ggattaattg agtatttaaa tgataataaa
     1921 gaagacttaa atgataatat agtatttata ttccaaccag cagaagaagg tcctgggggg
     1981 gcaaaagctt taatagatga aggagtgctt agaaaataca atattgatga aatatatggg
     2041 cttcatatat atccagagtt accagaaggt tatgtaggca caagagaagg atattttatg
     2101 gctcaaatag gagatattga catagaaata gtgtctaaaa gtggacatgg ggctatgcca
     2161 caaaatggta ttgatgggat agtgatagct tcaaactttg taagctcact tcaaactata
     2221 gtatctagaa atataagtcc aatagacaat gcagtgttga ctattggtag aattgaaggg
     2281 ggagctagaa gaaatataat agctgaaaac ataaagatgg aaggtactat gagatgcttc
     2341 aacccagaag tgtatgagaa aatgaagctt agagttagag aacttgctag agggtttgga
     2401 ttggcttata actgcaaggt taatataaat attatagatg actatattgc tgttaaaaat
     2461 gacaaggatt tgtatcaaga gtttgtagag gctattggaa gtgatactgt tgtagagcta
     2521 gagccactta tgatatcaga agatttttct tattatcaaa aagaagttcc agggttattt
     2581 tttatgttgg gttcaagaaa cgaagaaaaa ggatttgtaa atggtcttca taatataaac
     2641 tttaattttg atgaaaagat atgtgtaaat gcactgaatg tttattcaaa attattaaaa
     2701 tacaaagaag caatagatta ggaagataaa ttataaagat taaaaagcat aaatacctaa
     2761 atttaaaaaa agtgtattaa taaatacccc ttacatattt gtataaattt attttaattg
     2821 acaaaaatac aaataagaag gggtgatttt tttgaaagaa aaaatacaaa gagaaaagaa
     2881 gataactaat ggcaagaata ctaaaatatt gacaaatgat gatataatga aatatgagat
     2941 agcctcagaa ctaggtctta tggataaagt tggtgaacgt ggatgggcag gacttacagc
     3001 taaggaagca ggaagaattg gaggtatgct tacatcacgc aaaaagaggc tgaaaaagat
     3061 tgtggaggag cagaataaaa aggatgagtc aatatagcga ttttgcgttt gtatatgatg
     3121 aacttatgaa tgaagttgat tataatggtt gggttaaata tatagaagat ataatagata
     3181 gtgaaggtgt aaaagtaaaa aatatattgg aattggcatg tggcacgggt aatcttacaa
     3241 ttccattgac taaaaaaaat tacgatatag ctggtataga tatatctgat gagatgctta
     3301 gtgtagcaag agaaaaggca gaaaaagaag gagtagaatt agttttactt caacaagata
     3361 tatcagagct tgattttgaa atatctgatt tggactgtgt actttgtgct tgtgatggat
     3421 ttaattatat aacttatgat gatgatttag aaaatgtatt ttcaaagaca tatgaattat
     3481 taaagaaaga aggaatattc atatttgata taagttcttt ttataaatta gccaacatat
     3541 tgggaaataa tatgtatgga gaaaatagag aagatatagc gtatatgtgg caaaattact
     3601 ttgatgaaga agaaaatcta attgaaatgg aacttacatt ctttattaaa gatgaatgtg
     3661 gaaagtttga aagatttgaa gagatacacc aacaaagagc atatactgaa gatgagatag
     3721 ttgatatgct taaaaaatct ggatttaaag atataaaata ttatggggat tttacatttg
     3781 acaaacctag tgaagaaagc caaagaatct tctttgtatg taaaaaataa gatacttttg
     3841 gctatacaag ataattatta aaaataacca tgatataaaa atatagaaaa tgcagtttta
     3901 tagattaaaa gtaaagtgat gatactttat gacttttgct ttctaataat tataaagttc
     3961 tatagattaa aattaaaata gaatgactat tacttagtaa aactaaaata aaatgggttt
     4021 tgcccaaggt taatatagat aagtatttat aggttaaagt taaggtagta atataataaa
     4081 ataagacaat taacgggaca tggaggaata ctaatgagag attatgtact aagagctaca
     4141 tcaggaaatg gtcaagttag agcatttgtc gcaactacta gaaatacagt agaggaagct
     4201 agaagattac atgaaactac aaaagtagca acagcagcac ttggaagaac attaacagcc
     4261 acatctataa tgggattaat gatgaaaaat gatagtgata aattgactgt aataataaaa
     4321 ggtggaggtc caattggaac tattatcgcc acatcagatt ctaagggaat ggtaaaagga
     4381 tatgtaggaa atccgcaagt agaggttgaa gactatccaa atggaaagtt aaatgtagca
     4441 gcagcagtag gtacagaagg tgttgtaaaa gttataaaag atttaggtct tagagaacca
     4501 tataatggga cttatccatt ggtaagtgga gaaattgcag aagattttac ttattatttt
     4561 gctgtatcag agcagactcc atcagtagta gctttaggtg ttcttacaaa agaagatgaa
     4621 gtagagtttg ctggaggatt tatagtgcaa ttaatgccag atgcagaaga agagactatt
     4681 gctaagttag aggaaaatgt agccaaatta ccttctataa ctaacatgtt aaaagaagga
     4741 aaatcaccag aggatatatt aaatattgtt ttagatggtt tggagcctaa aatacttgat
     4801 acctgtgaag ttgggtttat gtgtgaatgt tcaaaagaaa gagtaaaaac tgctttggtt
     4861 gcaattggta aaaaatcatt ggctcaaata atagaagaag ataaaaaagc agaagttggt
     4921 tgccagttct gtaataagaa gtatatgtat agtgaagaag agttgcttga aattttaaag
     4981 gaaatgtagg ctataaaaat agttgtattt agataagtaa tgtcatagtg atatagtaat
     5041 gtcgtattga tatagtaatt aaagtataat ttattaataa tagatatatt tctattaaaa
     5101 aataagttta ataaattttt aagcttaata aattttaaga attaagaagc tttctggctg
     5161 taatatttgc aatgttgcag ttagaaggct tttttatttt attatagttc gtgtcaaaga
     5221 ttgtatgaaa caataaaata ttgtaaaata atttgtatat ttagacctga ttgacacaaa
     5281 ggtttataag atattttagt tgattatacg aaattctatg aagtagtaac cctgtgactt
     5341 taatcatggg aggttcagaa gaaggagaaa attttaaata tggaaagtaa aacgaagtac
     5401 ataggaataa gtatattaat attattgatg atatttttaa ttgcaaagaa ttttaatgga
     5461 aaacttgaaa tacctattcc tgagagtaat agtagaaaat ttaaatcagc agcagtgttt
     5521 tatcctcaac atcaagatga tgaggtttta tggggaggaa gtgccattgt agatgctata
     5581 aaacagtgtg gtgttgataa tgtatatgtg gtattagtat cagatggttc tggagttaac
     5641 gttttcaaag ctaacacaaa atttagaaac ttaactagga agcaaaagga agaacttagg
     5701 aataacgagt ttaaatcagc tttgagggag cttggagtga aacctcaaaa tgtgattata
     5761 cttgcggata tagataaaaa ggaaggcaca cattatgaat taatggaaaa gactatactg
     5821 gattttgaat ataagtttaa aggcaatgtg acccatatag cccatcacta taagtatgat
     5881 gaccatataa tgcatagaaa gaatggggaa gttttaaaga aacttaaacg tgaacgtaag
     5941 ataaaagatg acttgtattt tattaagcca aaatatgtaa agtatatacc aagaaatgaa
     6001 agagctatct ataaggcact ggattttgat gactataata aggttaagag tgcttgttat
     6061 gaatataagg ttgtaaattc aaagcgtaaa atgtatggta taggatatat ttcggcacat
     6121 agttattttg aacatctttt aaatgaccct gaacttacgt ctgtattaag tgaagattaa
     6181 tttcttataa gtagtaagtt atgtttgata agttgattag agttttctat agttttgaag
     6241 gtattgaaaa tttagtttta tttaaaaatt taaagataga ggtttttaat gtctggtatt
     6301 ttaaagttaa gttgaataat tgtatagaat tatttgtgaa agaagaaaca gtgtaaaaca
     6361 tagtatctta tttatggttt taaaatcata ggcaagatac tttttttatg taactaatac
     6421 cattttgagg ttattaactt aagaatttta tataatttaa cactcgtata taatgatgat
     6481 atttaggtag aatagatgca attacgctta aataaaataa ctgtattttt tataaaaatc
     6541 aaaccctaca ctatactacc agataaaatc tacatgctat aatttgacct gtgtttataa
     6601 aaaaggagta atctaataaa atagtatttt acttatgtat aactaacaac tattattacg
     6661 atttgctcta aagaatttaa agaaaaataa tgcaggaggt aaaatatgaa caatgaattt
     6721 ataattttaa aaggatgcaa agagaataat ttgaacaata tttccttaaa aataccaaaa
     6781 agaaaaataa ccatattcac aggggtgtcg ggttctggaa agtcatctat tgtatttgag
     6841 acaattgcaa aagaatcaca acgccaacta aatgaaaggt ttagtacatt tgtaagaagt
     6901 tttttaccta agtatggaga ggttaaagct gattgtatag aaaatctgtc aacaccaatt
     6961 atcatagacc aaagtagact tggtggaaat tcaaggtcaa ccctaggtac tattacagat
     7021 gtaaattcct ttttaagagc tttatattca aggtttggta gtgagtatat aggtaatgct
     7081 aatatgtttt cttttaatga tattgatggt atgtgcccac actgtcatgg gcttggaaaa
     7141 aaactagttc ctaatatgaa ccaaattctt gatatgaata aatcattaaa tgaaggtgct
     7201 atattgcttt cagggtttgg agtgggttct tggcattgga atatattcgc tgaatcagga
     7261 ttttttgata ttaataagag gatatgtgat tattcagaag tagaattgga aaagttttta
     7321 catggtaaag cagaaaagat taagatagaa aatgctggtc aaactaacat gacatatgag
     7381 ggattaatgg ttaaatttaa tcgtctatac ttaagtagag agggagaaat atctgaagct
     7441 actaagaaaa aattgagtaa acttcttata gaagatagat gtcctatatg tgatggaaga
     7501 agacttaatg aaagagtata taggtcttta attaatggct ataatatagc agatttaact
     7561 agtatgcaaa ttgatgaatt ggttgaagtt attataagta tagatgagcc agaagcacaa
     7621 cctttaatta aaggaataat agaaaagtta aacagtatca tagatatagg tcttggatat
     7681 ttaacactgg atagagagac atcttctcta tcaggaggag aatctcagcg tataaagatg
     7741 gttaagtatt taaatagtaa tcttgttgat ttaatgtata tttttgatga accaagtgta
     7801 ggacttcatc caaaagatgt gtataaatta aataatcttt taaaaaaact gcgtgataaa
     7861 gggaatacaa ttatagttgt tgaacatgac ccagatgtta ttaagattgc agaccatatt
     7921 gtagatgtag gaccaaaggc aggtaagtat ggtggagaag ttgtgtatga aggcagttat
     7981 gaaaatctac ttacaagtgg tacgttgaca gggaatgctt taagtagatt tcttcctatt
     8041 aaagaaagtg taagagagca tagtggatat ttagaagtta aaaattgtaa taaaaacaat
     8101 ttaaaaaatg tatctattaa gatacctaaa ggtgtattga ctctagttac tggagtagca
     8161 ggagcaggta aaagtacgtt gattaaggat gaatttttaa agcaaaatcc taatgctgta
     8221 cttatagacc aaagccctgt ttcagcaaat tcaaggtcga gtttggcaac atatagtgga
     8281 attatgaaca atataagaag catattttca aaaacaaatg gcgtaaatgc ttctttattc
     8341 agttcaaatt cagaaggagc ttgtgataac tgtaagggtt ctggaatagt agaaatgaat
     8401 cttgctttta tggagagtat aaagagtacc tgcaatgttt gtgaaggtaa aaaatttaaa
     8461 aaagaagttt tagaatatag gtttcaaggg aagaatataa ttgaagttct tgaaatgtct
     8521 gttttagacg caattgagtt ttttaattta aaacagatta aaacgaaatt acaatgtatt
     8581 gaaaaaatgg gtattggata ccttacttta ggacaaactc ttgatacttt gtctggagga
     8641 gagtgtcaaa gattaaagct tgctagtgaa ttaaataagg aaagttcagt gtatatatta
     8701 gatgaaccaa caacaggtct tcatatggct gatattgaga attttatagg tataattgaa
     8761 gaaattgtag ataatggaaa tacggtcata ataattgaac acaatataga tgtaattaaa
     8821 agagctgatt ggattattga gatgggtcct gaaggaggaa caaaaggtgg gagagtaata
     8881 tttgaaggta ttccaaaaca attatgtaac aataaaatat cattaacagc aaaatatata
     8941 gtataataaa tttatagtga tattttaata aatataatgg gtatataaaa tgataattaa
     9001 acttaacagt aataaataat gtgagaaata ttcaaattta gttaaataaa gtgtcagaaa
     9061 tttgtttaaa ttttagcatt tcaacagttc tgaccttttt tcttattttt atggagaata
     9121 tcgaggatgg agtctctaat agttactttt ttgtgtctac ttgcaagttt gattaaaaaa
     9181 tggctatgca cacttgtgac tttagtcatg ggaggttcag attataatct taatatataa
     9241 tttgtgaaat aatttgatga cgatttgctg atgtataaac aataaagatt aactacatta
     9301 aaatttaaat cttgttaagt aaaacttata aaaggagaga gatgaatgat aaatgatatt
     9361 ctaaccttat tatctgaaag aagtgatttt tttataaagc tatccataga acatttgatg
     9421 atttctatta tttctataat aattgcaatt gttttgggtg gtataattgg tattattgta
     9481 agtgaatttc ataattcttc taaacctata ttgggaacta tcaattttct atataccatt
     9541 ccatctattt ctatgttagg atttcttata ccattttctg gaattggaaa tactactgct
     9601 gttattgcac ttactattta tgcattattg cctatggtta gaaatacata tactggaatt
     9661 actaatgtag ataaaaatat tcttgaagca gctaggggaa tgggaagtac agattttcag
     9721 atactgtata aaataaaact tccactggct ctacctgtta ttatgtctgg tattagaaat
     9781 atggtaatta tgacaattgc attagctggt atcgcttcat ttataggtgc tggaggttta
     9841 ggtgtagcca tatatagagg aatcacgaca aataacacag taatgacaat tactggaagt
     9901 atacttattg ctctgttagc tttaatattt gattttattt tagggctggt agaaaaattt
     9961 tttcaacgaa aaagaaaaaa gaaaaatagg aaaaaagttt ttgccattct aggaagcctt
    10021 attcttatag taactatctt tacgtcttat ttacttaatc cttcaaagac tcaaactatt
    10081 catattgcta ctaaacctat gactgaacaa tatattcttg gtgagatgtt ggatatttta
    10141 attgaaaaaa acacaggtct aaacgtggaa attacacaag gtgtaggagg gggaacatct
    10201 aatatccagc cagctatgga gaatggagaa tttgacattt atccagagta tacaggtacc
    10261 ggctggaata tggtacttaa gaaaaatgaa ttatatactg aaagtatgtt taataaacta
    10321 cagtcggaat acaaaactaa acttaaaatg caatgggctg gtatgtatgg tttttctaat
    10381 agttatgcat tagcagttag aaaagaagtt gcagataaat ataatttaaa aaatatatca
    10441 gacttaaaag atgtatcttc taaattgaca tttggagctg aatatgactt ttatgagaga
    10501 gaggacggat atactccatt atgtaataca tatggactaa actttaaaga tacagttgat
    10561 ttagatattg gattaaaata tcaagctatc aatgaaggta agattgatgt aatgcctata
    10621 tttacaactg atggtcagta tagtgtttca gatgttgtag ttctaaaaga tgataaaaat
    10681 ttctatccat cttatcaatg tggaaatgtt gttcgttcag aggtattaga ggaaaatcca
    10741 gagctatcaa aggtatttaa gatggttgga gatattttga ctgataaaga aatggctaaa
    10801 atgaattatg acgtagaaaa caacaattta gaacctagag aagttgctcg tgagtttctg
    10861 aataagaaag gtcttttata aattggagga aatataatga cacctattat acaatttaaa
    10921 aatattaaaa aacaatataa tgataaaact ataatagata atcttaattt agatatagaa
    10981 aaaggagagt ttttgactgt aattggttcg tctggttctg gtaagacaac acttttaaaa
    11041 atgattaatg gtttgatttt gccagatgga ggaaatattt tgataaacca aatagatatc
    11101 aaaaacgagg acttaattaa attaagaaga agaataggat actgtgtaca gggaagtgtt
    11161 ctttttcctc atatgactgt tgaagaaaat atttcatatg taccaaatct tctaagtaaa
    11221 aaggataaat tagaagtaaa aagtgctgta aacaagtgga tggaaatagt gggattgcca
    11281 aatgatatga aagtacgtta cccatcagaa ttgtcaggtg gtcagcaaca acgtgtaggg
    11341 attgcaagag ccttagcttc ttctccagaa attttattaa tggatgaacc ttttggtgca
    11401 gttgatgaga ttacacgaaa gcaattacaa aaagagataa aagaaattca taaaaaaaca
    11461 ggagttacga ttatctttat tacgcatgat atttatgaag ctttgatttt gggaacaaag
    11521 actttggtat taaatcatgg agttgttcaa cagtatgata ctcctgagaa tattttaaat
    11581 actcctgcta accaatttgt agaccagtta ttgaatataa gaaaatctat aatagatgat
    11641 gaggatttaa aagaataaat ttgaatttaa aatcaaattg ttgtgataaa aactactgcc
    11701 ttagagctaa cttcaagttg tatgcttgaa gtgtacaaag ttagatttaa gatatatgag
    11761 ggaatttagc agtttgctgt agacgaggca actgttgaat gtgctttcaa taaatggcta
    11821 gcacatgtat ctgatgaaga ttataataaa ttgaaataaa aagttcaata tacagtagta
    11881 aataggcact tcaaattgaa gtgcctttta tgtgtcataa aatgaatcaa aatattagca
    11941 aagaatgtga aaaacaaaat aaaataatgt gctatcttta aaataggagg ggtgttatat
    12001 ggataacaat ttgattacca ttgaaaaaac tttaaattat atagaaaaac atcttttaga
    12061 tgaggtgtta gatttagatg tgatttctaa agaaataggc tattcaaagt accatttgca
    12121 tagaatgttt acatcagttg tcggatttag tgtacataat tatatacaaa gacgtagact
    12181 tactgaagca gcgagaatgc tcatttttac agaaaaacca attattgaaa ttgctttaat
    12241 ctcggggtat gatacacaac gctctttttc aagagggttt aaaaaaatgt ttaaacgtag
    12301 tcctaatttt tttagaaaac atcaagattt tttaccacta caattaaaat ttgatattca
    12361 taatagaaaa aaattacgtg gagatatgat tttagatgtt aaaaaagtta attgtgcaga
    12421 tataaatatt gttggctatt ctggtaaagt aaaaaaagga tttcatatta ttggaaaatg
    12481 ctggagggat ttgcataaaa ataaagagat gatagaaaat aaggtagatt taggtttttt
    12541 aattggagtt agtgattata gcaattttaa agagattgaa aatactccta catttacata
    12601 tattgcaggt gctcaagtag ctacttttgg caaattacct aatggaatgg aaaaattcgt
    12661 attaccgaaa agtaattata ttgtttttac ttttattggg aaaaatgagg atagtatgca
    12721 aaatgttgta gaatatatct atcaagaatg gttccctaat tcaagttgcc agtttaatga
    12781 aaataattta tatgactttg taaaatatgg agaaaaaact gatgagaatg gtttgagtga
    12841 aattcagttt tgggttccaa tcatttaaaa gaaggtatta tgtagatgga tttatatagg
    12901 caaagtttta taacagtatg tatttataaa taatttatag tctgtaggaa agaaataatt
    12961 ttactataag attatggtaa aatatacaat actatgacaa aatagtgtta aataatagat
    13021 ttaattttgc ataggaaaag ttctataacg gtgtgtattc ataaataatt tatagtctgt
    13081 aggaaagaaa tagttttact ataagattat gataaaatat acaatactat gataaaatag
    13141 tactaaataa tagatttaat tttgcatagg ataaggtgtt ttaataaaaa tgaatgtaga
    13201 aaacgaatta cttaaaaact tagatagatt acatacaaca gagttaggtg ttgtaagaat
    13261 taagaaaaat ctttctttgg agacaaatga tgtagtgaac tggtgtaaaa ctaaaataga
    13321 atctccaaat gccattatta atagaaaggg aaaaaactgg tatataagtg tatatgattg
    13381 tattataacg gtaaatgcta acagttatac aatcattaca gctcataaag aaaagaaatg
    13441 aaatgaattg caaaatacaa aggtcaagag cattttccat tgtttgtagt acctatgaag
    13501 aagtatataa aataatatta cttttgaata ataatttgaa ttttggaggt tgaaaaaatg
    13561 aatatactga tttacagtag gaccttgttg gagcgacttg gattttgcaa aaagaataag
    13621 ataaatatta gactagataa tcaatgttgt agtgaattat cccatgtcta gtgaatataa
    13681 ataacataaa aaaccgtcaa gatgaatagt ttattcgtgc ttgatggttt ttagtatttg
    13741 ttgaagtaaa attttatttc tttagcatat cacttttagt caatccattt gatttagctt
    13801 gagtattcca ttgatgattc cccataagtt tatataggaa tctagggaag ttaggtgaaa
    13861 taaaaatagt gcttttgttg gaatcattta tatgtatatt atcactaagt atattttttg
    13921 aaagttcttt taatgcaact cctaaatctt ttttttgacc tttacctaaa ggtacagatt
    13981 ctagtgaacc aaacatctcc ccagctccaa ttccaatacc ttgaatccat gtaaagttga
    14041 tttttttaca ccaatgctct atcatctgaa ttgccaaatg attatgttca ccttcattga
    14101 atccacagtt aactattgca tacactggaa taggtttttt attttgtgtt tttaaaaatt
    14161 tttcacattc ttctaaaaaa tgaagtaaat gagatggaat accatctaca taaagaggaa
    14221 atgcgattat taatctatca caattaagta tggcattaag agcgtcagga tacttagatg
    14281 tgttatctac tttaaatgta aaaactttat tatcattttg aataaattgt tttaattcag
    14341 ataaaaaata ctgtgaatta tttttattta cttttggact accatttatc attactatat
    14401 tcatatggat tcacctctta tatcttgtat attttcatag aaacaaacag agtgttcttt
    14461 tatattgaaa tttatagcat ttccatttac taatttcgta gcagtttctt tttcagcttc
    14521 tgtaatacaa tctccataga atatagcttt taaatttatc tgtttgtcat atctgctttt
    14581 atgacgcatc atattatctt ttattacaaa gtatggaaga atataattaa tgcttttatc
    14641 aagtacgttt ttgacaaaag ggctaaaccc tccataacag catctgctta ttataataat
    14701 tccatcagat tcatttataa gtttacccat gttgctgtaa taatctttta caacacattc
    14761 agtaggcgtt ttaatccagc agccaaaaca accaatacat ttgtggattt cttttgtgtt
    14821 tggaagtatt tttatattgt tatcaagctt gggtaataac ttttctaatt ctttgtcagt
    14881 taaatcatgt attaaaagcc tcataaattc catctccttt tattatgtat gtttaattct
    14941 aaaaattata tcttatagat ttttcagtaa atctttacac caattaataa caaaattata
    15001 atagtcaact ccataaatca ttgttgcata catatatgta tattggtcaa cacaatgatt
    15061 ttcttctaca tcaatttttt gtttacttaa tttatttaga agtaattcta cttcagttat
    15121 taacattttt aagtttgttt caattatttc tttgggtaga tacttgtaaa agaatatatt
    15181 aacaaggtgg tcttgctttg ttttagaaaa atttataggt ttctttaacc attcaataaa
    15241 tacttcttta ccagtattag ttatggaata tagttttttt aatttgctac catctataac
    15301 ttcgtgatag tgtatatatc cttttgtttc taatctttta agtgcaggat agatacttcc
    15361 aaagctggcg tcaaaaaagt acgaggtact ctctgacatt atttgtttta agtcgtatcc
    15421 acttaattct ttttccatta aaaatcctaa aataatgtat tctaacatat tgcctcctag
    15481 tatcaattag atatatatca attcgatata cttattattg cagataataa atattacgtc
    15541 aagatgcttt ttaaaataag ctaaaaagaa ttagaaatta ttaataaata tgcaaaaaaa
    15601 ttaatataaa aggtaattaa atatcaatga agacttaaaa atagttaaat caaagtaaga
    15661 gtctaatata taaaatttat agtaacttta tggtttaaat ataaacttgt gcgctattga
    15721 caaagtataa aattagcagt aaaataattg catgagcaaa tagattattg cgaacgcaaa
    15781 aaaataaaga caatagatat taaaggagag aatatgagag agctattaaa atgggtttcc
    15841 attataaata gatacaatac aaattatatt gacaggaaat tggcggatat tggaatcaac
    15901 aatagccaat atttttatgt attacacata tgtaataatc caggtattac acaggacact
    15961 atatttgaga aaattcttgt gaatcctagt aatataacaa gagctttggc aaatttagaa
    16021 aaagaaggct ttataacaag agaaccaggt gtaaaggata aaagaacatg gcatttatat
    16081 cctacagaaa aatctttaga atgttatgat gaaatactaa aaataacaaa tgggtatgtt
    16141 gaggagttgt tgagttcatt tgagaaagaa gagcaagagt tgtttatgtc aatgttaaag
    16201 aaagttgcat tttttgctat tgatatgaat gaagaagcaa aattggaggc gaaggaaaat
    16261 ggaagaaaaa tataaggagc cattggaata tgaaaaagtt tcaaagttat taaccaagta
    16321 cgcaattccc agtattatag caatgttagt aagtgcttta tataacattg ttgaccaaat
    16381 atttattggg caaggtgttg gggtattggg taacgctgct acaaatgtta cttttccact
    16441 aacaacaata tgtactgcaa ttgcattatt gttaggaatt ggaggagcgt ctaagtgttc
    16501 attggaatta ggtgctaaaa attcagaaaa agctacaaaa gcagcaggaa attctatatg
    16561 cttgatggct gtttttggaa taagtttatt tataatagta tcaatatttt taacaccact
    16621 attaaaattc tttggctcta catcagaaat actaccttat gcacacacat atacaaaaat
    16681 tacatcaatt ggattaccat ttcttataat gtccactgga atgagtaagt tgatacttgc
    16741 agatggaaga ccaaaagctt ctatgttttg tatgttaata ggagcagtta ttaatacaat
    16801 tttgaatcca ttattcattt ttgtttttaa tatgggaatt gctgggtctg ctttagcaac
    16861 tgttattggg caacttatat cttttggaat tagtgttcat tatataaaga attttcaaca
    16921 tattaaatta cataagaaaa gttttaaatt agatagtata atttataaac atatatttgc
    16981 actaggttca agttcatgtt ttaatcaatt agcaatgact gtaacacaga tagttatgaa
    17041 taatacattg gcatattatg gggctaggtc agtttatgga agcgagattc cacttgcttg
    17101 tgttggtatc attattaaag taaatatgat ttttatatct ataattattg gtatatcaca
    17161 aggtatgcaa ccaattatag ggtataatta tggcgctgaa aagtataatc gtgtaaaaga
    17221 agcatataag tttgcagtgg gaagtgcaac tattatatct gttattgcat tcttgtgttt
    17281 ccaattattc ccaagacaaa ttacgtctat atttggtaac ggaaacgaga cttattttat
    17341 atttgcagaa cattatttta gaactttctt gttcttaaca tttataaatg gtgttcaaat
    17401 agtatcatca actttcttta catcaattgg taaatcagtg ttgggacttt ttacttcttt
    17461 gactcgtcaa attttatttt taattccatt gatagtaatt ttaccgatgt ttatgggaat
    17521 tgatggagta atgtttgcag gaccaatagc agatgcagca gcggcaatgg tatgtatata
    17581 ctttgcagtt catgaaatga aatcattgac tataaagcaa aataatttga tgagtaacca
    17641 taataagatt gcagaggtat aagacaatat ttaagaagta tagaaaatta atttaaaaag
    17701 tataataagc taatttaaga aacatagaaa attaatttaa agaaatacaa taaattaatt
    17761 tttaagtaac ataaattaat gtaaataaaa taacgttcaa gtgaagattt aaaggtgcat
    17821 tttctggagc taagtatatg ttgtagagtg ttacgtctct ctagcctgtg aagtttaata
    17881 gctcaagaaa atgcattatt tatgatgctt attttgtttt atttctaatt ttagaaataa
    17941 ctattctaat taaatctaaa attataccat aaataagaaa agaaagtata gatgctatgt
    18001 accaataaat cgaaattgac cctgtaattg ttctacatgg gatttgcatg cctgaatcca
    18061 taaaaacaaa aaaactatca atggtagaaa taatagacaa aataggattt aatcctatta
    18121 gaataatact ttttgagtct tgaccaattt ggtgcatata aatttcaaat aatgcaatta
    18181 aaaaaaaaca aaatgtaaaa gatttaataa tttttttcat caaaatacct cacctaatat
    18241 aaattctgtt tgcactattt gcttactata actatttatc tccattcagt cacatcactt
    18301 aattgtaatc taggtcttct tgatggttct tcatcaggat aacctaaaga tattgctgcc
    18361 actaattgac tatctgtatt taaccattca cataattcac gatatgcaaa gtatacatca
    18421 caaatccaaa gactacctag acctaattcc acagctgtaa gcgacatatt ctgaattgca
    18481 gctccaattg attgcatgtt tgccatttca tagaatcgtt cttcagggga tagattttct
    18541 aaaggtgatt tacctaatat atttaaaata aaaattgtta ctggagcttg tttcatgatt
    18601 tcaaccgtgt aatttgcacc agcgatatgt tgacgacttc ctggaagaag cccattatca
    18661 ttaatttcat tttgaatacc tttgctcatg gctttaagca tagattcttt ttccttttca
    18721 gtgatgacaa caaaacgcca tggttgcctg tttttagatg aaggcgcatt tattccagct
    18781 tcaattattt tctctatggt ttcatgtgaa atgctttgat ttttatattt tctgatactt
    18841 cgtctttttg atatagaatc acttatcatt ttaagtaacc tccgtttatt ttttggaaaa
    18901 atctatttat tttctatttt taattatttt agcattaatc agaaaaaaag tcattgattt
    18961 tagtatacaa ttattaatga ttgtattaag aattgtatca aaaagtatgt gattatacta
    19021 agggattata ttagacatta tatttaaaat tgtattaaag gttgtattta atattatatt
    19081 aagacttata tatattaaga tttatatata ttaggattta tattatattg cgccttatat
    19141 ttaagggcta taacttgaaa ataagctata aaaactcaat attgttttgt tcagagataa
    19201 aaaaaggata ctttaggtga tttttattaa aaattaagag tgataagatt atatacttta
    19261 taaatgtaaa tatgacgaat aaaaatgata aatataataa tctatatatt tacattagga
    19321 gttttctata ctataattat ttaaaactaa atataaatgt tggaattatt taggacttga
    19381 aattgtctaa aagtaaatat aagtgatgaa gaggtgatgt gatgagatta gatatggata
    19441 gtatgttata tgtactaatt tatatgacat tattggtatc tatcattgga agtatatttt
    19501 gtttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaga gacatatttg
    19561 taaatagaac tacatattct gaggaaaggt tgcctaaaca gtatatagtt tttcagaaat
    19621 atgatatttg gaggtattgt agtttgtttt aatttgaaat gaatctatta taaattctaa
    19681 agtttaaaaa taaaaaggag agaataaaat ggatttcatt tcaagtatat tgcaaggatt
    19741 attaagtata ttatttaatt ttacaggtga ttttggaatt tctattgtac ttataacttt
    19801 aatagttaaa ttagtactta tgccactatc attaaaacaa agattttctg taaaaaaaca
    19861 gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataaaaatg atactaaaga
    19921 attggaaaag caaatacaag taagttctgt agaaagtatg aagagtatgc tgggatgcct
    19981 tatagttttg gttcaaatgc cagtagtata tgctttatat catgtatgtt tagatatgac
    20041 aaaggaattt acaacaatta taattccatg gattgctaat ttaggtacat ttgataattt
    20101 gtttatatta ccttgtatat atacgttaat tatgttagct tcaaatttag tgaattatat
    20161 accatattta aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg tggtgactac
    20221 tataacaagt cttatactga ctgttagaac tccagtagcc ataggtctgt actttataac
    20281 aagtgcaatt tattcactaa ttgaagatat ttgttttaga atatatttta atcgaaaaaa
    20341 tagaatagtg acaaaataat ataattatta ttagaaatat tttagcctgc taacatagga
    20401 aaagttagta ggctattttt atctatgata tcaattccat taagaatagt ctaaaaaata
    20461 ctatcaaaat actattaaaa aatagttgaa aaataaataa attaatataa actaaaataa
    20521 agtctacaaa aaagaggtga taaatagaaa tggctaaaat attagttgta gaagatgaaa
    20581 aaaggatgca aagtattatt gtagaatata tgcaaaaagg aggatatact tgtataactg
    20641 ctgatgatgg agtcgaggca cttacaatat taaagagcaa taatattgat ttaatgatat
    20701 tggatattat gatgccgtat ttagatggat tttcagtgtg tagagtttca cgtgaaatga
    20761 ctaatatacc cattattata ttgacagcta aaggtgaaga ggaggacaaa ttaaaggggt
    20821 atgaatatgg tgcagatgat tatataacaa aaccatttag tccaaaagta cttttagcaa
    20881 aagttaatgc tttgttacgt agatacacta ctgatattcc aaaaaattca ttgagtttag
    20941 gaaagatttt tattatggtg gcatcaaggc aagtgtatgt agaagataaa ttgattgatt
    21001 taacatataa agaatttgaa ttattgcgtt tatttatgga aaaccctaat caagtttttt
    21061 ccagagaaaa acttttaaat tgtatatggg gatatgattt tgaaggaaac acacgaactg
    21121 tagatacaca tataaaaacc ttacgaaaaa agcttggtag tgaaggacac catatagtta
    21181 cattaattcg ttcaggatat aagtttgagg ttaaggaatg aatagtttaa gaaagattgt
    21241 agaaaaactg aaaaatgtag atatttcaaa ttggattaaa ccaaattttg gactatattc
    21301 agttagaaaa aaagtgtttt tactttctaa attatcaggg gcgttaatta tattatttta
    21361 tttgattgtt gaagaatttt cgctaaactc taaatttgaa tttttgattt ggtttataat
    21421 actggttata tttattatga gtattgattt tttattggga aaatttattt cagaaccaat
    21481 cacatctata aataaatctg caaaaagtat gtcccaactt gatttttcta atccgtgtac
    21541 tgtaaatact aatgatgaat ttggagaact ttctaggagc ttgaatacaa tgtctacaaa
    21601 tctacaaaag gcattatcag acctagaaag tgcaaatatt caacttgaaa aggatgtaaa
    21661 taaggaaaga atgctattgg aacaaagaaa ggaattggta gatacaattt cacatgaaat
    21721 gaagactcca cttggtatta tacgtgcgta tacagaaggg cttatggatg aagttgatga
    21781 agaaaaaaag aaaaattata tgaatgttat catagaagaa actgatagaa tgaacaatat
    21841 gattgtatca ttattagact tatctgcatt ggaagcagga gtttctaaac ttaacccaga
    21901 gcgttttgat tttatagagt ttgttgaaac tgtggcaggt cgtcttttaa ttgatatacc
    21961 agatgccaat ttttatttta catatgattt gccagagtat gaagtttttg tagtggcaga
    22021 taaaatgaga atggaacaag tagtagaaaa tctaattatt aatgcaaaga agcatgttgt
    22081 atatgatggt aatttagatt tatcaattac atgtgaaaat ggcttgcttt tatttgaaat
    22141 ttataatgat ggtaggatta tagagcaaga tgagatttca aagatttggt ctaagtttta
    22201 tcgtagtgtt caatctcagc gtacaggagg ttcaggtcta gggcttgcaa ttgtttcaca
    22261 aatactcgca atgcaagggt tagaatatgg agtgaagaat cgcgataaag gagtagaatt
    22321 ttattttatg attccaattg atgaataatt tagtcttaat ttataaatta taagtatttg
    22381 atgtaaaata tttttctttg tagacaatag atatctaaag agaataaaat aaaaaagttt
    22441 aataaaaaaa gcttaattag aaagtctaat aaaaaaattt aataaaaagc ttaatgagaa
    22501 gtcttgtttt cctgatgaaa acaagatttt ttattaagct ttttattatt tcacttcgat
    22561 ttcacaagag agacactttg atttcaccaa atagaaataa aataagttaa ataaattaaa
    22621 aaaatataag aggtaattaa atacgcttac aagggggggc acttatgaaa tggtatacat
    22681 ggttgactat tatattggta gtattggttt ctattccgct taaaattaaa tttataaaat
    22741 ggtgggatag acgacaaaaa gaaaaaaatg atgaaggagg ctatgataga tgataaaagt
    22801 agaagagttg aaattttctt atggaaaaga taaacagata ttgcatggac ttaattttga
    22861 agtaaaagaa ggagaaatat ttggatttct tggacctaat ggttcaggaa aatccacaac
    22921 acaaaaaatc ttaaatggtg ttttaaaagg ttacaatggt cacgtatcca tttttggtaa
    22981 ggaagttaaa gcatacacag aatctttata tcaaaagatt ggtgttttat ttgaatttcc
    23041 ttatttatat accaatttaa gtgcaattga taatttagaa tatttttcgt ctttttatcc
    23101 taaaaatcaa aggagagata tatatgaact cttagatttg ttagagttta agaaagagtt
    23161 tacaaataag cctgtatcat cctattcaaa aggtatgaag caacgtatta gcatggcaag
    23221 agcattggta agtaatccaa agttgttgtt tttagatgag cctactagtg gacttgaccc
    23281 atctggagct gtactatttc gtaagattat tgaagaagaa cgaaaaaaag gaacaactat
    23341 ctttttgact actcataata tgctagatgc agatttaatg tgtaacaatg tggcttttat
    23401 tgcagatggc aagattatgg caatagataa acctaaaaat ttaaaaatga aaaatagcaa
    23461 taataaagtt gaagtggaat ttttatataa tggaaatcga gatattgaaa ttgtagatat
    23521 acaagagtta gaatctggaa taacttttaa atacgataaa atattaagtg tacattcaaa
    23581 agaacctaca ctagaagaag tttttattaa atatacagga aggatgttag tgtaatgtat
    23641 tctaaatttt ttaccttagt aaaaaaagat ttttggatga tgatttatgg taaatttttt
    23701 gtactaacac ttggctctct tatactttat tcgtgttata ttaattttgg ttatgtaaag
    23761 tttatggatg aagatagtta tcacaataat gtatatctat ataatccaga gaatataagc
    23821 atgagtgcat ctccattaat tcatcaagtt tcttctatag atgaattaaa agcaaaactt
    23881 gatgaagata caaatgcagt ttgtattgat atgagttcca gtaagccaaa agtaatttta
    23941 catgaaagta ttaaaggagt tgacaattat agagctgact atgcattgtc attattatca
    24001 tcagaaaaag accataatag caaaatatcg gacaacaatg caataattca aattaatgat
    24061 aaggatacat ctgagataaa acaaagaaaa gaaatgactt gtgagatttt attctttgaa
    24121 attgtagcta ttggattttt agggattgcc tctttattat ttaaagaaaa acagatgggg
    24181 gtaatgcgta ttactggtat tttacctgta aaaagaagcc tttttatatt atcaaagata
    24241 tttgcttttt tgatatgtga cctatgtttt acagtgcttt tagtagtatt taatgttgga
    24301 ttttttgaag gaataaaaat acttccacaa gtattagttc aagctagtat cttatcaatt
    24361 attatgtctt tgattggaat aggatgttca atgttattga gagattttaa gcaattctct
    24421 cttatatacc ttgtggttgc tgtattcata actacaccta tatttctatc tgcaaataca
    24481 tcttttgaaa tagattggat taaatatcat cctttctatc atgtttatag tcggcttaaa
    24541 aatgcgtttt tcatgaattt ttcaaaagaa ttagtttatt ttgtagtgtg ttctttgaca
    24601 ataatagttc tatttttact tgtaaaatat atttttaata aagagttggt tagggaggga
    24661 tagtcttgat aggattacgt taccagttaa agagtataaa aaaagataaa atgtgtataa
    24721 tttccttctt tttaccagtt gttatggcga ttttaataaa ttttatggga acaattgata
    24781 tatcttcatt aggagagata cgttttggta tagtgaaaga tacacttcct gtacaaacag
    24841 agcagtggtt gaagaagtat ggtatggtaa gtgtatatga cacaaaaatg catttaatac
    24901 gtgaaattaa ggatattaaa acggacacaa ttggagttgt tagagatggt aaagggataa
    24961 aaactgtgct ttatggaaat gagattaatg ctataaaaaa tgttggaaag acattgccag
    25021 agatgtttag actaaaggga gatttaaaag atgttgaggt tacaatattg cctaaagata
    25081 gtattttaaa agagtttcaa aatgttttta ttgctatgat tttaattact gctatgttta
    25141 tgggatgcac ctttaatgca atgaatatta tatcggaaaa agaagatgga atatcattta
    25201 taaatgaaat tttaccacag agtagaagta agtatattat acaaaaagta tttttgggtc
    25261 ttttatgtgg gtgtctatct tcaatgataa ctgtatttat atgtttggat atttcaatat
    25321 tagatatgat aatgatgttg ctactcgttg tattttcagc atttatttca tcacttatag
    25381 gtctatttat tggaaagttg tcagaaggtc ttatggtagg aattgtttat attaaaatag
    25441 taatgatttt atttataggt gctcctttgt taatatatct tctaggggtt aatacaagtg
    25501 gtataggaaa tttatgctat ataatgccat cagttgctac atttaatggt attatggaga
    25561 ttattaatgg taatgtcatt attataaaag atattgtaat tttggctgta cattgtgtag
    25621 catggtttat gttgtatatt attttggata aaaaaagaaa gttatctttt agtagataac
    25681 tcagtataat tataattagg acaatttaat aggtgattta atatactttg tatagtgtaa
    25741 tttatgtagt gcaatttgta tactgtaatt tatgtagtgt aatagttagt tttattttat
    25801 aattatt
//
LOCUS       DAEMAE010000035        25583 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEMAE010000035 DAEMAE010000000
VERSION     DAEMAE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.035"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(180..860)
                     /locus_tag="KPH93_001160"
     CDS             complement(180..860)
                     /locus_tag="KPH93_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1687991.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEIVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKIKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(1012..2919)
                     /locus_tag="KPH93_001161"
     CDS             complement(1012..2919)
                     /locus_tag="KPH93_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1687992.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYSTL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIISIIVIGYGYY
                     LSTNIRTGDITSVLPIIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNILRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKVGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2920..3672)
                     /locus_tag="KPH93_001162"
     CDS             complement(2920..3672)
                     /locus_tag="KPH93_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1687993.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMLN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3990..5144)
                     /locus_tag="KPH93_001163"
     CDS             complement(3990..5144)
                     /locus_tag="KPH93_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1687994.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDKINNYLSFIKTFGSIGWAIGSMVASYLILRFSYS
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTSKDIIELLKDKKYVLLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITIQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSVNIGYNYTLMVLSLFGCLAYGLIVSLNYLQE"
     gene            complement(5147..5614)
                     /locus_tag="KPH93_001164"
     CDS             complement(5147..5614)
                     /locus_tag="KPH93_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1687995.1"
                     /translation="MFFKRKKEVMTINSYIDGKIINLEEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5628..6116)
                     /locus_tag="KPH93_001165"
     CDS             complement(5628..6116)
                     /locus_tag="KPH93_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1687996.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMICFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6130..7461)
                     /locus_tag="KPH93_001166"
     CDS             complement(6130..7461)
                     /locus_tag="KPH93_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1687997.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVDKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPATLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKKGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDIVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7475..9040)
                     /locus_tag="KPH93_001167"
     CDS             complement(7475..9040)
                     /locus_tag="KPH93_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1687998.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYVHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFAFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9209..9994)
                     /locus_tag="KPH93_001168"
     CDS             complement(9209..9994)
                     /locus_tag="KPH93_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1687999.1"
                     /translation="MRLEEMIFFNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDELCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10160..11359)
                     /locus_tag="KPH93_001169"
     CDS             complement(10160..11359)
                     /locus_tag="KPH93_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG1688000.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFFNDKKVVDRYDSMNPKIKMPDDIFVKKELILKDVIRG
                     QVGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWKGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVESLPANVKENAPKEIIKDFNGFE
                     DTSYTIKSTDDLKFRGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKSGVCDEEFKTTPEFKVKDCKRTADFKEDNCIRKAL
                     ELENINY"
     gene            complement(11882..13219)
                     /gene="xylA"
                     /locus_tag="KPH93_001170"
     CDS             complement(11882..13219)
                     /gene="xylA"
                     /locus_tag="KPH93_001170"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG1688001.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKANMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKVNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13319..14866)
                     /gene="xylB"
                     /locus_tag="KPH93_001171"
     CDS             complement(13319..14866)
                     /gene="xylB"
                     /locus_tag="KPH93_001171"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG1688002.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNVSSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLIYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPVSENVDTYKIYHRLYKRIYKSLKDDFRLLDE
                     VVNCKNK"
     gene            15074..16234
                     /locus_tag="KPH93_001172"
     CDS             15074..16234
                     /locus_tag="KPH93_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG1688003.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16390..16794)
                     /locus_tag="KPH93_001173"
     CDS             complement(16390..16794)
                     /locus_tag="KPH93_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688004.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(16815..17297)
                     /locus_tag="KPH93_001174"
     CDS             complement(16815..17297)
                     /locus_tag="KPH93_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688005.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVDAGLKI
                     HSINVGNMSSRKDTTVLKPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17317..18126)
                     /locus_tag="KPH93_001175"
     CDS             complement(17317..18126)
                     /locus_tag="KPH93_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1688006.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18119..18898)
                     /locus_tag="KPH93_001176"
     CDS             complement(18119..18898)
                     /locus_tag="KPH93_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1688007.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSII
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18898..20976)
                     /locus_tag="KPH93_001177"
     CDS             complement(18898..20976)
                     /locus_tag="KPH93_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBG1688008.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHIWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(21589..21762)
                     /locus_tag="KPH93_001178"
     CDS             complement(21589..21762)
                     /locus_tag="KPH93_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688009.1"
                     /translation="MTGYVANISQTSQALCGIRSINGTIPVVLTVAMLVLIKMYSLAE
                     RKCREVINELRSR"
     gene            complement(21933..22454)
                     /locus_tag="KPH93_001179"
     CDS             complement(21933..22454)
                     /locus_tag="KPH93_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG1688010.1"
                     /translation="MNRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELIDKINDIQMVDEKGIFIEELEKLLKEYNLE
                     IPIVGFDELKEDK"
     gene            22620..23186
                     /locus_tag="KPH93_001180"
     CDS             22620..23186
                     /locus_tag="KPH93_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1688011.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(23403..25466)
                     /locus_tag="KPH93_001181"
     CDS             complement(23403..25466)
                     /locus_tag="KPH93_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG1688012.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVITVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKDDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
BASE COUNT         8498 a         4446 c         2675 g         9964 t
ORIGIN      
        1 aaaatttata taatctataa caaaaaatac cattattata aacagcacta aaaaatctaa
       61 gtatattaac tctgaatatc tatttttaaa aatatctata gcaaataaat agatatttat
      121 tatactaact acaactaaag gtagagcaat atacttagac ctacctttta aatattttac
      181 tagcattgta ttatatatcc tatccccttc ttagttttta taaaatcatc aaaacctagt
      241 tctttaattt tacttctcaa tctattcata tttacagtaa gtgcattgtc tgtcacaaac
      301 tcatcattat cccatagagt catcataaga ctttcccttg atacaaccct attcttattt
      361 tttaataata atgttaaaat ttttatttcg tttttagtca actctatact tttatcctta
      421 tacttatgtt ttataatccc tttccccaca tcaaacatca aatcttcaca atacaaaata
      481 ctattagaat cactataatt atagactctt cttattatac catttatttt ggtaaccaat
      541 atgtctatag aaaatggttt ggtaatataa tcatctgctc cattatttat acccattatt
      601 aaatccatat cagaatccct tgatgataaa aatattaatg gtacatttga aatagctctt
      661 attttttcgc accagtaaaa tccattatat aatggtaaat ttacatctaa aagtactaca
      721 tctggttttt tatccataaa ttcatctaat actatctcaa aatcatcaat taattctact
      781 ttaaatcccc atttctcaag tgctaacgca atctcttttg ataaatcaat atcgtcctct
      841 actaataaaa ttctatacat ttgtatattc tccattatcc taatatttca ctatgccacg
      901 aattattaat atcataacat attttatatg aatttattac ccaaattatt tagttaatca
      961 acaaggagac tcttattaca agaatctcct ttctctttat taatatatca actaattttt
     1021 atacactaca ctcttaaact tattaacaga tactgctcca tatattatga atattatcaa
     1081 acatatcact aaacttagta tatatatacc aaataagtcg gtcatcaata aatctcctgc
     1141 taatctcaat gcaaaataac tagatatagt tgcacttaac attggcaaag tataaaatat
     1201 agctacttct ttaagtatag ctctatttat atctttttct gtagtaccta cttttaataa
     1261 aatcttgtat ttttctttat cttcatatgc atcactatat attttcatat atattatact
     1321 tccagctaca attgcaaaga ctaagaataa aaacacaagc actacatatg caaacttcat
     1381 ccattgactc tcaccttgag ctttatatcc attctgaata tatccatttt ttaaacttaa
     1441 gttcttttca atattgttga acattacttg gctattttct tcttgaaata cctttgcacc
     1501 ataaaaatat acttgttgac ccttatctct taatttgaaa tatgtatcgt catttactac
     1561 tatgatactt tgattaagtc ctgtccctag tactggaact tttacttctc cccttaaaat
     1621 attatataac tgaccatcaa gttttatctt ggcttctttt gtagctattg atgctatcgt
     1681 ttgagggctt tttaactcta ttacattatt tccactaact aatctttcac catactcttt
     1741 taattcttta tcattgccat tcactttaag agtattacaa aaatcactat atttcattat
     1801 agtatctgtt gtatcataag cttcttttat atctgttttc tttaaaattg ttagttctgg
     1861 atttactgag tactttatct tattagtatc ttttattata ttctctattt gttttttatt
     1921 cacttcatct ttatccattg aaactatgct aatagtgtac acatttcttt gttcttgggc
     1981 attcttatgt atacgcttca tcgcaactga agcaccaaga gttgatattg ttgtagttat
     2041 aattatagct attatagcat aagttttata gtttttattt aatcggtagt ttatattgtt
     2101 tattgcaatt atattatttc cattaaataa tattttttta tttttgatta taatatcaaa
     2161 tataacaggc ataactgatt tgaataagcc ataagtacct actattatta tacctataat
     2221 aggtagtaca ctagttatat ctccagttct tatattagta gaaagatagt agccatatcc
     2281 tattactata attgatatta tagctaatat atatatacct attcctacct tcggtatttt
     2341 ttcttgtttt ttagatatat tgattagatt tattacacta cttctacata ttgttataaa
     2401 accttttaca gccattatta taaatataga aataaatatt aaagtagaat atagtatagc
     2461 ttctatgcta agtccattag atacctttat attaaatcca gataacttta ttatcactac
     2521 ttgaaaaaat ctagatgtta ttatccctat aattattcct gttatacaag aacttattcc
     2581 tataagcata ttttctacaa aatatatttt tcctatagta tataaatcta atcccataaa
     2641 ggacaatatt ccaatttctt tagacctatt cttaaaaaat atatttgttg cataccatat
     2701 aaagaaaaat aaaaatactg atagtatcat tattacagct actaacactg atagtgtaaa
     2761 ttttttattt tcctcttgta acacatttat agcatcacca tatcttaata attcaaaatt
     2821 agttagcaca actactgaaa aaaccataga tataaaaaat gctagataag ttcttatgtt
     2881 actcttaaaa ttattataag caattccaag tatactcact tacatctcac ctcccataga
     2941 ggctagcata tccataatct ttccataaaa ttcttttcta ctatttcctt tgtttagttt
     3001 acactttata tttccatcat ttagcatata tacttcattt gaatagcttg ccgttagagg
     3061 gtcatgtgta atcattataa tagtagcgtt gttttctctg tttatttttt ctaatgattc
     3121 taaaagttca tatgctgatt tagagtctaa agcacctgtt ggttcatcag caaatatgac
     3181 tgctggattt gtaattaatg acctagctgc tgcacctctt tgtttttgtc ctcctgataa
     3241 ctgatatgga tatttatcta aatgattttc taatccaaat tttttggcta tagaaaatac
     3301 cttagcttct atctccttac ttttcttttt tcctaatgct agaggaagtg ctatattgtc
     3361 cattagtttc atattgtcta atagattata gtcttgaaat ataaacccta ttttttctct
     3421 tcttaatttt gatagttcat tattttttac ttttgatata ttaattccat ctagtattat
     3481 ttctcctgaa gtttgtttgt caagtgtaga cattaaattt agtaatgtgg tctttcctgc
     3541 accagatgga cccattatcg ctataaaatc accttcattg actgtaagag atatattttt
     3601 taacacattt gttttaaatc cttttattcc ataatctttt gatatatttt ttatttttaa
     3661 tatttctttc ataataatag cctccagttg ctaatttttt aatatatctt tattttaaag
     3721 gttttatata aataaatcct taagtaaata ttacatttta actgttagca ttacactttt
     3781 gtaacatatg gaagactatt ataattatag tggaaatatc ttaaaataaa agcatgattc
     3841 acttttttgt ataaacaaag aaaagaagcc actaagaggc ctctaatcta tacaataatt
     3901 cttctctata aaaaaacaaa aatacaacct tgattgtggt aaatacaggt actataaaca
     3961 aatgatatta ccactatata ttaaaacaat tattcctgaa gataatttag tgagactatc
     4021 aagccataag ctaagcatcc aaaaagactt aataccatta atgtataatt ataaccaata
     4081 ttaacgctca aaaaaccagc aacagttgga ataatcagag atgagcctcc cataaatata
     4141 cttaaagcta ttaattggct actacttcta agttttttag gagaaacttc aaatattaat
     4201 ctttttgaag taattaaaat taaaggagta gaaaacactt gaaaaacaca cagtataata
     4261 atgatatttg aatcatttgt taaagcaaat aatagaaact gaattgttat cataatagca
     4321 cttaatttca ataagtttaa actgctatat ttttttaata aataagaacc agccagatat
     4381 gttggtatct ctaataatga ctgtaaagac cactttaaag atatttgaga atttgtagca
     4441 tttaaagcta tcattttatc tattacagta cagttatttg ctaccaccat tgagtataat
     4501 aaaaataaaa tacatactag taagacatat tttttatctt ttaataattc aataatatct
     4561 ttactagtta catttatttt cttattaatc ttttcaatat caggtaataa tagtatattt
     4621 ataataagta atactacaat taaaccaata gctacagata atccagaata tgagaatcta
     4681 agaatcaagt aagatgctac catacttcca atagcccatc caatcgaccc aaaagtctta
     4741 ataaatgaca aataattatt aatcttatct ccacaaccta ttatccatgt atcatataaa
     4801 ctacaacaag tatttaataa ccctcctgac aaagcaacaa gtaataagta tattataaaa
     4861 gttgcagatt cagtagaaaa cataataact gatgcaattc cataaactga taaactaatt
     4921 aaaacaatct ttttcattgt ttgatactta tcagctaata ctccaaataa taattgaaat
     4981 aatatattgg ttattgcata gctcgataat ataattcctc tttcaaatgc tgagtaacct
     5041 aattttgata agaaaggtgt gaattttgtt gacgcaaagg caaaaataat atatgagaaa
     5101 aaatagaata tgataaattt aaatttaatc gctttattaa tcataatcaa tcaaaattga
     5161 taactttaat accactattt ccttcctgat ttaagtttga aagatgatat tttaccgtat
     5221 taggattctc tacaataact aaaggaatat ataaagtatt ttcaagcaac ttattatcaa
     5281 tttctactaa cttttccccc atattaactt cttgaccttg tttaacttct aatttaaagt
     5341 tttctttttt tgttgttgca ctatccaatc caatatgaat aagaatttgg acatcatttt
     5401 gcaatttaat tcctatcgca tggttagttt gagcaataaa acttattact cctttagtag
     5461 gtgatttaag aatattgtcc tttgctatca tagcaaatcc gtctcccatt accttagttg
     5521 aaaatacttt atcaggaatt tcttcaagat taattatttt tccatcaatg taggaattaa
     5581 ttgtcattac ttcttttttt cttttaaaga acattgtaaa cactcctcta cataattaac
     5641 tttattctaa ttatcattac aaataacgat attaagcata ctgcaattga ggaaaaaaga
     5701 gccttatagc tctttcccca ttgtagttct ttaatactgc ttatgatttg tttaattcca
     5761 acaaatttat aaatataggt aaacatgata acaatagtta ttgtgtaaat ttggaatatc
     5821 tgccctaata agactaaata gaaagcaatc atgttttctt ttatctcttt attctttttt
     5881 atattataaa taacatatgc actatatact gcactaaagt tagagattaa atttaaactt
     5941 gcattaacat cattgtttaa aagttgtgaa aatgaaatgt ttgtaaataa tgatgtcaat
     6001 tctaaataca ctatatataa aacaggtaat attagaaaac aaatcatcac cactgaaaga
     6061 aatatatttt catcttttaa tactgctgtg atatttttta ttttatttgt tatcatattt
     6121 ttgttctcct tatttaaaag ttggccagaa atccttatta gccacaatca tttcatctaa
     6181 gatttctttt gctacttttg cacttggaac agttttactc atagtaaacg actgccataa
     6241 tttttgataa cttttttcta tccatgcttc tacagctaat ttttcagaag caacttgttg
     6301 ttccattaaa cctttttgga aagttggaat tttacctata gacataggtt caactatatc
     6361 tttcccaaca aggcaaggaa tttcaaccat tgcatcatct gcaaaatttt caatagctcc
     6421 attattagga acaatcagta aaaatctttc ctgagtatta aatgctaatg cacaagctaa
     6481 ttcaacaata aattgagaat gttcacttgc atcaaaccat gtatctcttg ctgtgccctt
     6541 ttttataatt ctttcacatt cttcgaatgt atccttttct cttctatcca taatttcatt
     6601 tgctcttgta taatttggat tagcatgttc aacagtttca tcagccatta aataatattt
     6661 taaataagta ttaggtaatg tagctggttc tatttctaat aagtcttttg cggctctcat
     6721 agtttctagc caactagatt ctttgtgttg accttgaggt gtaaattcct catatccttt
     6781 tttagcacaa tattcctgta atttaggcat taaatcattt ccttctttgt ccttaatact
     6841 tgtccaccac ccgaaatgat tcaatccata gtatctaaca tcaaagtctt tacgagaatt
     6901 taaacctaaa actctagcaa atatttcctc taaacatact ggcatatcac aaatattaat
     6961 aattcttgaa tttggtctta atacacgaca tgcttctgca actatagctg ctggatttga
     7021 gtaattaagc atccaagcat ttggagaata cttttccata taatcaatca tttcgattat
     7081 tggacctatt gttcttaatc cataagcaat tccacctgct ccacaagttt cttgaccaac
     7141 aaccccatat tttagtggaa ttttttcatc ttgttcacgt aaagcatata aaccaacacg
     7201 aatttgtgcc attacaaaat caatatcagt aaatgcttct tctggatttg ttgtataact
     7261 aaatttaatt tctggatttt tctctctaac aattattgca catgcttcag caacttttct
     7321 ttgtctctca tcattattat catataattt aattgattca ataggaaatt tatcgacact
     7381 ttcaattaac attgttacaa ttccagcagt ataagtactt ccccctccag caattagtat
     7441 tctctgtctt tttctttcca tctttccttc tcctttataa taaattttca aattcttctc
     7501 ttacacttgg tactgaaaga ccaataataa tctgaatagc ctttccatta tgcactaaac
     7561 catgtgcacc tactgcctta aaatcttctt ctttagaaat aattgagtca tctttcaagg
     7621 ttaatctcaa cctcgtagca cagtttgtaa catctacaac attttctgct ccaccaacta
     7681 attttatgta ttcagatgct tgttgagagg ctttactcaa tttatttcct ttttcatctt
     7741 tagtttctaa gtactcattt ttagtatata gttttgtttc ttcactatct tcctctctac
     7801 caggagtttt catatcaaac ttcttaatta agaaagcaaa tacaaagaac cagataaatg
     7861 aaaatataat ccctacaaca acttgaacaa catatacctt ccagtgatac attccaagag
     7921 gaagccaatt taatacaacc cagttaataa ttcctccacc aaaatcacca acaactccta
     7981 aggcaaacga aatcgtttgc aagcacgcat ctaaaaatgc atgtactagg aataaaagtg
     8041 gtgctacaaa taaaaatgta aattctaacg gctcagtaat tccagttaat actgcagtga
     8101 gagtagcagg aatcattaaa cctaatactt ttttcttttt ttctggcttt gcagttacat
     8161 agaaagcacc aaatacacct aatgaaccaa acaccttcga taatccggat aatgaaaatc
     8221 caattggata catttccttt agagtcttag ctgaagtttg aaagtcctga atatgagtag
     8281 cccaatatac tgatgtacca cctggaacaa caacactatc ataacaaatt ggtgcatata
     8341 caaaatgatg taatccagtt ggtattaaaa tcttttgtaa gaacgcataa caccaaactc
     8401 ctatatttcc actatttata aaaaacgttt gcaaatgtct aataacattt tgaatatgag
     8461 gccaaataaa acaagttaat aatgcaactg gtatcataac aaagaaacaa attgcaacta
     8521 caagtgatga gcctctaaat aatccaataa aatctggtag ttctttatca taaaatctat
     8581 tatgaacata aatgacaata cctgatatta acaaagcacc aaccatacca gtatctaatg
     8641 ttttaatacc agcaatgctt acaagtccac ttccagcact tatttcagct gcgaaatcaa
     8701 ctccaaagct ttttccccaa aaacttaaca tagaacctac aaaataatta aaagtcaaat
     8761 acgttgctaa agcctccata caagctcttg cttgttgctt ttttgcaagt gctattggta
     8821 aagaaatagc aaataataat ggtaattgat taaataccat ccaagctcca ctagatatta
     8881 caccccaaaa ttggtaccaa atattttctg gtgtagcaag tgaacccata attgactgat
     8941 tttgaaatac aattgagaat ccaaccatta tcccagcaaa agcaaataat agtgttggtg
     9001 taaacattgc ccctccaaaa cgttgtattt tcttcatcat ataaattact cccctcctca
     9061 tatttttagt ttatctttgg ccttagatat ctattatttt aacaggttaa taaatatata
     9121 caatctttag ggtacattcg gagcttattc acagtcaaat aagtttttgc acatttttat
     9181 gttcaaattc tcataaaaaa aagaatgact acaatttgca atcaatccct tctgtaatca
     9241 ttctatgcct tttataaata ctatatttaa taaataataa ctcaatcaat ataaaatata
     9301 acattgtcaa tttataacta gggtgcattt gtaaaaatga gatattagtc gctgaaatat
     9361 aaattgattc atcacttaaa tcagtcaatg tattattttt aaactctgta attgaaatta
     9421 ttttagttcc tattaatttt aatcttctag caatgttaag tgtattctca ttctcaccac
     9481 ttagggatat aataatcata acatcatttt tactgacaat attgtaaaaa gaatcatcaa
     9541 atgttcttcc ctcaaaatca tatattattt cttgacatga taagaacatt ctctttatct
     9601 gttttgcaac cacattttga atatcacctg aaccataagc gtatattgtt cttgattcat
     9661 aaagaagttt acacaattca tcataatctt tatttttttg atcttcaaca tacttaatta
     9721 ttgaattgca tgcaatatca aatgactgat tttctataac atgaaccttt tctgattccc
     9781 agcttaataa tactttaaac tcactgaaac cttttaaatc taatttttta acaaatcttg
     9841 taatagttgc acgagacaca ttacatttca tagcaagttc atttattgac atcttactac
     9901 aatgtttttt atttgcagtt atatatttcc atattaataa atctgtatca ttcaatttat
     9961 tataattaaa aaaaatcatt tcttctaatc tcataaacta ttatcccctt taattaaaaa
    10021 tcgtcataaa tattatacca atattcaaca ttaaattaca gcatattctt atatttacta
    10081 tttatcccat aaaattgact ataaaaaata ctgctataat tgatgtttaa aaaatgtagt
    10141 tttaattcta ttcttttatc taataattaa tattttcaag ttctagtgct tttcttatgc
    10201 aattatcctc tttaaaatca gcagtccttt tacaatcttt tactttaaat tctggagtag
    10261 ttttaaattc ctcatcacat actccactct tatttaaata catacaactt gccattctaa
    10321 ctattagacc actatttttt aaattaaaca atacagggtc tatacaccct ccatctccac
    10381 cagtagtttc accaactaaa gttgcaaatt tagtttcttt acaaaacatt gcaaatgact
    10441 ctgatgatga atatattttt ctatcaacaa gtaagtatat attaccccta aattttaaat
    10501 catctgtaga ttttatagta taggaagtat cttcaaaacc attaaaatct tttataattt
    10561 ctttaggtgc attttccttt acatttgctg gtaaactttc tacaggattt aattttatat
    10621 ttcttgcatt tgtatatttt tttactattt cattattatt tctataaatt ctataaccat
    10681 ttattttgac atcatttttt atgagtttag atacgattcc cttccaatac ctatcacttc
    10741 cacctaaatt acctcttata tcaataatta aagctttatg tttttctaaa gtgttgatat
    10801 aatctccaat tatttttaaa tcattattta tagacccatt cttcgatgac attgatggta
    10861 aatacatata tccaacctga cccctaataa catcctttaa aatcaattct ttttttacaa
    10921 aaatatcatc tggcattttt attttaggat tcatagagtc atatctatct acaacttttt
    10981 tatcattaaa aaaatcgtac caatcattat tagagtatga tttcttaaaa agttcatatc
    11041 ttttcttatt atctattact tctgtgtgtt tattatttaa atcagataat atagatgaca
    11101 gttcttctat aaattcttca tcattttcag tgttttttat tcgttttgta tattcatcct
    11161 tatttgctaa ccaatcaata ttattaagcc tcttatttac acctagatat ggatatcctg
    11221 tctttacaac attatacaca taattaaagt catctacctt ttcctcttca gacaactgat
    11281 tcttttgaga ataagtagaa ttacctaata agattataaa tgctaaaata attactaaaa
    11341 ataatacttt ctttttcatg taattctcat ccactttcag attttatatt ttaatacatt
    11401 gtatcattat tttattatca cctaaacaac taaaagaaat tcttgaagat atattccatt
    11461 attattctct ttagaaaatt tttatttgta ttttcaaaat tatttcatga aagttataaa
    11521 ataacaatca tattcaaata tctattttac tgccatatcc tgaaagtaaa ttcttatcag
    11581 tgatatacta aaaaagatat gctaaaaaat ttatccatta taatgactta aatttagata
    11641 ctttactgat ttttatttat tagttgagaa agatattgat aattaaaaat attgataact
    11701 atagaaatga ttatttttta tagaaatttt aaggatataa actaattata cttaagtcta
    11761 tatccttaat ttatcatgat tatgagaatt tttacaaata cttatctttg tccgttttag
    11821 aataaaagca agaatatatc ctgttatttt attcaaacat ccaagtttaa ttactacaca
    11881 attacttgtt agaaattgta tctacttcat atatatatct gttaagtata gcttctagca
    11941 tttcttgtct tccagactta ttctttataa ctggcatttg cagtgcataa gattctaact
    12001 ctttaaatcc aaccttacct tctactatgt ctttacctat accttcacta aaacttgcat
    12061 atctttcttt agtaaagttt tcaaatacct catcttctaa taatttatga gctattaata
    12121 atccctttgc aaatgtatcc attcctgcaa tataagctag gaataagtct tctacctcaa
    12181 aagatgctct tcttacttta gagtcaaaat taaatcctcc tggtgctatt ccaccttgct
    12241 ttaacacttc atacatagca agtgttgcat catatatatt tgttggaaat tggtctgtat
    12301 cccaacctaa taataggtct ccttgatttg catctatact acctaaaaca ttgtttattc
    12361 tcgctatatt taattcatgt tggaaagtat gtcctgcaag tgttgcatgg tttgcttcta
    12421 tattcacttt gaagtattta tccagattat actttcttaa aaatcctaaa acagtagcag
    12481 tatcaaaatc atattgatgt ttagtaggct cttttggctt aggctctatt aagaattgtc
    12541 cagtaaatcc tatttcctta gcataatcta cagccatttg taaaaatctt gcaaagttat
    12601 ccatttcaag ttctgtatta gtgttaagca aagtttcata accttctctt ccaccccaga
    12661 atacaaaatt ttctcctcct aagtacttag ttatttccat agctttttta acctgagctg
    12721 ctgcatatgc atatacatct gcattacatg ttgtagatgc accatgaaca aatcttggat
    12781 ttccaaacat attggcagtt ccccataata actttatacc tgtcttttcc atattagctt
    12841 ttatatactc aactatttca tctagtatct tatttgtctc ttctaaatct cttccttctg
    12901 gagctatatc tctgtcatga aaacagaaat actcaattcc catcttagac ataaattcaa
    12961 accctgcatc aactttaatc tttgctatct caatcgggtc tgttatatta ttccaaggtc
    13021 tctccactgt gcctactcca aatgggtcat taccctctcc acataaagta tgccaatagc
    13081 tcattgcaaa tcttaagtgt tctttcatag tcttattacc taccacttgt tcaggattgt
    13141 agtatctaaa tgctaactca ttgtcagatt ttactccttc aaattttatc tgtcctattc
    13201 ctttaaatat ttcactcatt tcatgtcctc ctaatatttt acacatataa tatatgtagt
    13261 atgttttctt aatttaaaac tattttctat ctttattttt ctttttaagt tattttaatt
    13321 atttattttt gcaattaact acttcatcta ataacctaaa gtcatctttc aaagatttgt
    13381 atattctttt atacaatcta tgatatatct tgtatgtatc tacattttca cttactggtt
    13441 ctaatttagt tgaagttttt attatagcat cacaagcttt ttgtatattt tcatatattc
    13501 cagctcctac taatgcaaga attgcaactc caagtgctgg tccttcactt gaatttatag
    13561 tatatacatc ttgattaaac atatctgctt gcatctgtct ccatacttta ctttttccac
    13621 ctccacctga ggctcttact tcattaacgt ctatattaag attttctatt atatccatac
    13681 agtctttaag actatacgaa acaccttcca ttattgacct tgcgatttct tttttagaat
    13741 ggattggaga taatccaaag aacacacctc ttgcataagg gtcaagatga ggagttcttt
    13801 ctcccatcat atatggtaag taaatcaatc cattacatcc tgttggtact tgacttactt
    13861 gattatttat aatctcatat acatcttcac ctaaagaatt tgaaacttcc acttcacttt
    13921 gacagaaatt atctttaaac cattttaatg acaatccagc tccttgagta actcccataa
    13981 catgccatgt atttggtatt gcatgacaga aagtatgaac tctaccttct ttatcaattt
    14041 ttggctcttt tgtataagca aatactacac ctgatgtacc tatagtagat gaaactactc
    14101 catctttaac tattccattt cctatagctc ctgctgcttg gtctccagct cctcctacca
    14161 ctattgttcc ttctttaagc cctgtcaaac ttgctacatc tctagtcaca taacctgtaa
    14221 tctcttgtga ttcataaact ttacctaaaa gatttttatc tatatttaac ttttctagta
    14281 cttcatcgct ccaatctctt tttcttatat cgagcaactg cataccactt gcatctgata
    14341 cctctgtagc atattcacct gtcaatttgt atcttatata atcttttgga agaagtattt
    14401 tattagtatt tttatatata tctggttcat tgtttctaac ccataatatc tttgatgctg
    14461 taaatcctgt aagtgctgga ttagcagtta tttcaatcaa tctttcttca cctactaaat
    14521 cagttatctc ctcacactct tttactgttc gttggtcaca ccatattata gagtttctca
    14581 atgttttacc ttcattgtcc agcataacta gaccatgcat ctgtcctgat aatccaattc
    14641 cttttataca ttcactgctt acatttgaac tttgaactac ttctcttata gattcgacta
    14701 cagctttcca ccaatcattt gcatcctgtt ctgcccaacc tgatttctct tgtataagag
    14761 gatattcata tgtacaactt tttatagtat ttcctagttt atcaaataaa actgttttag
    14821 tacctgatgt accaatatcc acacctaata catactccaa cttcatccct ccttgaatgc
    14881 cacttcataa ctatcgtttt cctatcctaa tttttttata ttttaggtat attttacttg
    14941 ctttattttt gttagtatat taaactaact tatgaactaa taataaaata tttaattatc
    15001 tttgtcaatg caaaataacc ttttattttt gtgtaaaaag tattacaata ttattttaag
    15061 gaggaatatc aaattggtta ctgataaata cactattcga gaaatgaatg aaagattggt
    15121 acttgaacaa attataaaaa atggtcctat ttctagggca agtatagctt ctactatagg
    15181 attgaataaa gcaactatat ctgctattac caaaaaactt attgatgaat ctctagttca
    15241 tgaaatagga attggaaata gtactcatag tggcggaaga aaacccatat tattagtatt
    15301 taataaatgt gcagggattt ctttaagtat ggacattgga tacgactata tattttcttc
    15361 tctatcttac ttagatggaa ctattataaa ttctaagaaa ttaacagata tacaagtaag
    15421 taaagacaat gttatacaat tgatagatga aattataaat agttataaca tatccaaaat
    15481 agacactcct tataaggtaa ttggtcttac tttggctata catgggatta cttgtgaaaa
    15541 taaagtattg tttactccat attacaatct taatgagatt gacttatata gcatccttag
    15601 caaaaagtat gactttccta ttcatataga aaatgaagca aacttaacag ctttagctga
    15661 gaatactttt tcaactgtac ataactcact acttagtctc agtattcaca gtggatttgg
    15721 ttcaggcatt ataataaata ataaattata tagtggcaga aatggtatga gtggagaaat
    15781 tggtcacact atcatcatgc ctaatggtaa actatgtcct tgtggaaacc gtggatgttt
    15841 agaacagtat tgttcagaaa aaaaggtatt tgaacagctt tcttctttag aaaatattcc
    15901 taaaatagat tctgatatag taaagcaact atattatgaa gataaccaga atgccaaaaa
    15961 agttattcat gagttttgta gttacttaac aatagctata aataatgcta ttacaactta
    16021 tgctcctgaa ataatatatt taaatagcca aataattagt gatatccctg agatattaca
    16081 gattacaaaa gatatgttag ttagctcttt taacaaagga ataaatatag aaatttcatc
    16141 attagggtct gaggcatctc tatatggtgg ctctgccgta aatatcaaga gctttttgaa
    16201 tatacaaaat ttaactttaa ttaatgaaat ataagggtta tgactatata cagccataac
    16261 cctttaaatt atacattcta aaattagatt tattatatag tttatgcaag attagctata
    16321 ttttttaaaa attgtatatc aattaagtat taacacaagt attttgtttt aaatattaat
    16381 ataatcttcc tactcatcat cagataataa acttgcattt tgttcaacca tagaaatatt
    16441 atctttacct attttctcta attttgctgc caattccttt gtatctaaaa aagctgattc
    16501 aataaccata cctaaattta gccctgaaat aattttaatt cgactgtcat tattggctac
    16561 taataaagca gcattgcatg gagttccacc tagtaaatct gccattataa ttatttctga
    16621 actttccttg tacacttcta aagctttttt taatttttct attgttcctt ctattccatc
    16681 atcttgtttc atatttacag taggataatc tatagaatcc cctattatca tttttgcaga
    16741 atttaagact tcttcagcca tattgccatg actcatcaaa ataatactat tcactttaat
    16801 acacctcttt aaatctataa gatgtctaca agataagttt tcttatcatc aggcgtcatt
    16861 atagttgtaa tttcaattcc ttcatccaaa agtcttttaa atgcttcttt ttcttcttca
    16921 gtaacactga tattaggttt tagcactgtt gtgtcttttc tacttgacat atttcctaca
    16981 tttatactgt gtattttaag tcctgcatct acaaatggaa taaggtccac aggagactta
    17041 acaattaata atacattttg cccttcatac tttcctgcag taatattttt tatagcagtc
    17101 tcttctgtaa ttatagatga cgctatacct gcaggtgtag ccattcttaa aacttgtttt
    17161 tgaacagaat tgttagctac tgcatcattt actaccatta atcttgtaac acccaattta
    17221 ttactccaca tagttgcaac ttgaccatgg attaaacggt catctatacg aatattttta
    17281 atacctttca tttccatatc taatatcatc ctttccttat tttaaaatac ctaatgcata
    17341 taaaattatt gaaacaacca ttactcctaa aattaagtat ataggtttta tattcttttt
    17401 aagagcccaa taaacaatca caactaaaat tgcaggaact aatccaggca taatttggtc
    17461 taatatttct tgacctttca ttgtaaaatc accttgttga aagttcaaag caacattagc
    17521 ttttacaact gaaggtatta atgcacctac aaccattaat cctaaaatag atgtactctc
    17581 agttaaaatc tttaaacttt tttcaattga agatattagc ttagtacctg atgtataacc
    17641 agcattaaat aatggtatac ttaataattt caatgctaca ctcactacaa tccacagtaa
    17701 gattccaaat ggacttccat ctatcgccat attagctgct atagctccaa aaattgtcca
    17761 cggaataacg acaaataaac tatctcctaa acctgctact ggtcccatca agcctgtttt
    17821 aattgaagca acagcctctt cagtctcttc acttgataat tcctccattg ctacattaat
    17881 acccaaaata taaggggcaa cccaaggatt agtgttaaaa aattgtaaat gatttaatac
    17941 agcactttgt aacttttctg gattatcttt gtatactact tttaaaaaag gtagtactgc
    18001 ataacagtat gccaaggcct gcatcttctc ataatttgaa gaaacagaac tagtaaaaaa
    18061 ccagcgccat aagacacttt ttttcacctt ttcatttgat ttatttaatg ataccttatc
    18121 actcatcttc gtccatacct cctactacag tatattgttg ttcttgattt tccaaattct
    18181 ttttgtaaat tataagtgca attgctccac caattaaagc tacacctaat aatggtactt
    18241 tcatatatac tgctaaaaca aaaccaatta ctaagtaact aaaatactcc ttagcaggaa
    18301 gatatctaag taataatcct atccctactg ctggaagtag tcctccagct actgttaaac
    18361 cacctgttaa ccattcaggt gtatatttca aaatggtatt aattaaagaa ggtccgaata
    18421 ttatagatag agcaactgga atacctgttg tagctgctgt taatagtact cctaacattt
    18481 gcatataggt tatattttta taatttcctt ttttagctcc atcttctgct ttatgaacta
    18541 gccatatacc aatagtattt ctaagaacat ctaattgtac catcaataaa gcaactggaa
    18601 tacctataga gattcctaca gatgcttctt gcttagtcgt aatagttata aatgtggcta
    18661 ctatacttgc tgtttgataa tcaggtattg atgctccacc aaagttacta atacctaatg
    18721 ataatagttg taatgtacct ccaatatata accctgtatt tatatcacct aagataagac
    18781 cagtaataaa acctgctgtt actggttgat atgttccaaa cattgttgaa tttttttcat
    18841 aatttataaa aaatccatat agtacgatta ataataattg ccataattgt ggctccatta
    18901 tgatttcacc tctcccaaaa tattttttag ttctttaaaa ttataactgg aatcttgctt
    18961 taggaaaata ggaatttctt ccagtgggca ttcaacttca taagtcttcc caccttcata
    19021 gctaactcca ctaaatatat tttcccatat atgtcctttt ggtaaatata cttttctact
    19081 tctatcttta taattcatta ttggagctac caaaatttca tctccaagca tatatgtatt
    19141 atccacctcc caagttttat catcttcagg aaattcataa aataaagttc tcataactgg
    19201 tgttccgtct tcatgcgctt ctttcatcag tttttttaaa taagttttta agctctctct
    19261 aatttttatg aattttgtaa gaattacttc aacttcttca ccataagacc atatttcatt
    19321 aggagctcca gttaccattg agcctccacc tttatttgat aatggtttac tatgaggcaa
    19381 tctatctcca tgcattctca aaattggtga aaaagtagca tattggaacc atcttaccat
    19441 aagttctcta aattctgggt ctttaggatt tcccccatgg aaacctccaa tatcagtagt
    19501 ccaccatggt atacctgcca gtcccatatt taaccctgta tttacttgat tattaaaagc
    19561 ctcaaaacta gaatcaatgt caccagacca tactaaagcc ccatactttt gagctcctgc
    19621 ccaagctcct cttacaagag ttacaacaga ctctatgtca ttatgtaaac catcataaat
    19681 catttttaaa taatcaatag gataaatatt accacaactt aaaacatttc cttttttata
    19741 tctatagtta tcaaaatcat acacagaata gccaggttca gctacatcta accaataata
    19801 gtcaatccca agttgcttat aatttttatc tattctatcc caaacatatt ctcttgcatt
    19861 ttcatttgtc atatctacaa agactgtatt tccttgaatt tgcattgtca tcctaacacc
    19921 acgattaaca ttgactaaat aaccattttc taaataatca ttgtagtttt ctgcatcact
    19981 ttgaactgta ggccatacag atactattgg ttctactgaa tactcttctt tcaatttttt
    20041 aactaattct ttaggatttt tccaataatc taagtcaaat ttatattcac cttgcttagg
    20101 ccaatgaaaa tagtcaattg caattgatga aagcttaatt cctcttttag aatattcctt
    20161 cacaacatct aaaacttctt ctgaagttct atatcttagt ttactttgcc ataatcctaa
    20221 aaggttttca ggcatcatag gaacagttcc tgtaacttga gaataatttt tatttaactc
    20281 ttttgggctt tctccacaag taatccaata atctataaaa tttgtagaaa acatcttcca
    20341 ttctgtcata tttttagcaa aagatacttt tccaattccc ggattattcc ataaaagtcc
    20401 ataacctaga ctagaaatat aaaagggaac tgatatttga gaatttcttt gggctaactc
    20461 caaaacagta ttctttaaat ctaaaaattt atgttgatat tgtcccattc caaaaatctt
    20521 ttcggaaggc tcagattcaa atctagtagt tacttcaaat tctccactat agcttggtcg
    20581 atattctctt gattttaact ttaaagtaga gttaaagtct ttggtaatct caattgtccc
    20641 tacatcttct ccaccatcat catgttttac agctcttaat cgaatatatt ctttcaataa
    20701 tatttcattt ttatcattat aaaaagtaat tctatctcta tggtctaaga cagcttttat
    20761 ctttccattt ttaatgattg cacttccatc ttcattttta ttaattgtaa tatcatcttt
    20821 atttaactta ggtttttcgt taagagcata gttttcatca acaaaatttt ggtctacaaa
    20881 cgacctaata cgtaaagagt tttttcccca tgcttctatt cgcaatagtt cattgtcaaa
    20941 tcttttttca atatagtctt cagatatttt tatcacggct acctcctaaa tcaatggtat
    21001 aatattctat ctttgtgtag cttaagttaa ataatgtcgt ttagtatcgt tatttataaa
    21061 ttaggtttta tatataattc atttgtacat taaactaact taccaataat atactttatt
    21121 tgactttgtt tgtcaatcgc tttccggatt ttagtaaaat tttctaatat attgatggaa
    21181 agtcattctt aatatttttt atttatactt ttaatatctg tgatgatacc tatatacatc
    21241 tagatttttt aaaacacata tgggaataac gtttgcctat tatgtagata atatcgtact
    21301 tgaaaatctg tatagtgacc ttataaatac agataataat atattagata ttattcaaaa
    21361 caatggattt tataattata aataatttat gaaattattt aagaaaatat ataattgtat
    21421 accaagtcaa aaacgacaac aaataaataa aactaaaaaa gatgttgtct caaaatatag
    21481 ataaatttat ttttcattgc taaaacgatt aaaatatatt tgatttaata taaaaaaaga
    21541 gtgtctcata aactaaaaag ttcactttta gacactcctt tatatatatt atctacttct
    21601 taattcattt attacctctc tacattttct ttcagctagt gaatacatct ttataagtac
    21661 taacatagct actgtaagta ctactggtat agtaccattt atagaacgaa taccgcacaa
    21721 agcttgtgaa gtttgagaaa tatttgcaac atatcctgtc ataaatagta ccaaacatca
    21781 atatgaatat taatgataat attgattttt ttaaattttt ctttaggagt actaattcct
    21841 ccacttaaaa atggttcatg tactgtccat cagtcatatt tttctaccaa atatgatggt
    21901 gtttcataat gagacaataa atatttatat ctttacttat cttctttaag ctcgtcaaat
    21961 ccaactatag gtatctctaa attatattct ttaagtagct tttctagctc ttctataaaa
    22021 atacctttct catccaccat ttgtatatca tttattttat ctatcaattc attgcaacca
    22081 cttaactcac caaaccagcg tttagaacta catgtcttat tataaccaca acttggacta
    22141 ccatctattg caatgatact ttttatatca taatcatttt ttaaatattc atcaaactgc
    22201 cttacatgag gtattaacat ttgcctacat tgttctctaa aaaacaaatt gtcaaactgt
    22261 tcttttacat gtccccacct atttattcca tacataatca tttctgggca tggtagttga
    22321 attattccat accctttatt tattaaatct attgctatat tttgtgctcc cttaaattct
    22381 gacaatccct ctactttaga attacaattt aatatacaat gacataccac tacaatctct
    22441 ctatttctat tcataataat ctccttagtt attcttaatt ttaatgatac tacatatttt
    22501 gataattata ttatttattt gttctatatt tccaatattc tatatagaaa tatacaatta
    22561 gtaatctttt ataatctata ctatgataaa tgtataaatt tttttaggag gtattataat
    22621 tggatactaa aaaagtcaca ttttcttcaa tgtgcatagt tattaatatt gtcttaggta
    22681 tggttatatc tatgataaat ataccattat tatttttaga tacagttggt actgtacttg
    22741 gggctgttgt gttaggtcct ttttggggtg ctcttattgg cgggtgtaca aatttagttt
    22801 taggtgttat atcaggacct acaaatatac cttttgccct tgtaaatatt gttcttggtc
    22861 ttattgttgg atatatttct aagaaaaaag gttttggtta taaagaagca attattacag
    22921 gagtaatttt atctattgtt tgtcctttaa ttggcactcc tatttcagtt ttattgtttg
    22981 gtggtttaag cggaagtggt gctgatttat tggttggttt tttagttcag tctggtcaga
    23041 agatatttac tgctgcgttc tttccaagga ttcttagtaa tatagtagac aaacctttgt
    23101 cttgtattat ggtggtatat tttataagta aaatgcctaa aggttttata aaacaatata
    23161 gtaaaaatac ttctagttca tattaataaa tcatttatta tgtaagattt attactcttt
    23221 aaaatgctat atactcattt ttatataaca tttttattaa taatttaatt agatagtatt
    23281 tgtattattt caattaaacg taacctcttg gtataagttt gagctataaa tctatatcaa
    23341 gaggttctat tttattttta atataattaa tattctatta cgcgcaaaac gcatatattt
    23401 cattatttta tggaaaatat tacttcattt aactctatat caaactcatt aagtaaaaat
    23461 ctaattatat ttaatttagc ctctgtattt aaatttgttt ctaaatatat attttctgat
    23521 attttaatac cttttcttaa tttatataca tctctagact taatattatc tataaaatct
    23581 cttttataag ttttgtctac aatactgtta aaccttacag aatccaaatc atataaagta
    23641 agagcaactt gttcataaaa ttctgtccaa tttttagcct tatatgactc tcctaatata
    23701 ctaaatgata ctatactttc atttgtaaaa ttaacatcat catcctctag cgtaaatata
    23761 ttttcactgt tacttggagg ttcatatgta acacttggat atttccaaat ttctacagct
    23821 ctatctagta atatcttagt tctatttttt atttcttctt cattccattt atctatagat
    23881 gaaatatatt tatttagata aagcctacta tctttaaatc catcttccat ttctttcttt
    23941 tcttcaaatg ttttattgct taattttgaa ttatatcctg tcaaagttaa atttccaatt
    24001 gtatgtaaat atttatcatg tatctcctta tagttatctc ctagtgattc cttccattta
    24061 tttgttaatg tctgtggcat tatatgctct atatttagca tattattatt gattaaattt
    24121 tctaaatcaa caatttcttt attattataa ttctctaacc tctcaagtat atataactta
    24181 ttctttgact tagtattata tatatcttta gtttcaaaag catttgaaaa ttcactgtca
    24241 gtaggaaatc tttgagatga tttcttattt ataaaaatat atcttaatat attagaatag
    24301 ttttctttat aatcactaaa ttttttaatt tcccttccta agttcataaa tactttattc
    24361 agtgcattcg gaggtacatc acatactatt cttcttaata taaaacattc tactaaatta
    24421 agggattcta caagtacttt tttatttatt ataccttcat tataatcatc aaataattct
    24481 aatataaacg gatatgatac aaatatttca agtttgttta tccttttcaa aactttgttt
    24541 acatcattat catcagtaca tgaataagat atctctctat aatagttaga aaatttaagt
    24601 aaatctttta ataaaatttc tatatctata ccattatctg atacatacct tttaaaacta
    24661 aaatatactt tatttttatt cggtatactt ctctccttta tagtcaaata gtctcttata
    24721 aaactactta cgttgctact tgtatttttt tcaattttat accaataatt attataaaat
    24781 tcttcttgta acttagagct tctacccatt aacacaaagt ttcttactct atcagcttca
    24841 cttaaatcta agccagttga atttagactt tcaaagatta attgagggtc atcttcacca
    24901 ttttttaact ctatttccac tatagttaat ttctgtatag catcaaacaa atcatctatt
    24961 gatatattat cgtcttttat tctattataa aaatataaat aatttgaaac tatattagaa
    25021 tttctatctg tattcatttc ttcttcaaat agttcatagt acgcttttct gtcttctgct
    25081 attggtttta actttatttt tccatcttta tctcttgagt gcttgtttat aagataacac
    25141 tccttaatct gttcttctgt tgtaataatt ccttttgatt ccccaaactc ttttattatt
    25201 tgacacatgg caatcaataa tattgacaca gtaatcactc tttgttgtcc atctattatt
    25261 aaatattctt ttcccatact atatatagat acaactgacc ctaagaaatg agttctaaat
    25321 ccagaatcta ttatatcctc taaatcatca aacaatcttt tacattgttc tttcttccag
    25381 ttataatttc tttgatatac aggtactaca aaacttttgt ttgacccctc taaataacta
    25441 agaaattcaa tttctcctgc tttcatatta actcccccta taagttaaat aaattccatt
    25501 cataactcca tgtatctata tattatatca aaattttgac tcataaatgt atatgaaagc
    25561 cttattatat acctttttat acc
//
LOCUS       DAEMAE010000036        25098 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEMAE010000036 DAEMAE010000000
VERSION     DAEMAE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.036"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..1966
                     /locus_tag="KPH93_001182"
     CDS             110..1966
                     /locus_tag="KPH93_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG1688013.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSDYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEVLIDKIFESDKGVIENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKEDGKINIFL
                     YVKDNIFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2048..2288
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2354..2589
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2674..3891
                     /locus_tag="KPH93_001183"
     CDS             2674..3891
                     /locus_tag="KPH93_001183"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG1688014.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4008..4490
                     /gene="cdeM"
                     /locus_tag="KPH93_001184"
     CDS             4008..4490
                     /gene="cdeM"
                     /locus_tag="KPH93_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG1688015.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYERAAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYQGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4755..6020
                     /gene="hisD"
                     /locus_tag="KPH93_001185"
     CDS             4755..6020
                     /gene="hisD"
                     /locus_tag="KPH93_001185"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG1688016.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEIAQEVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6225..6977)
                     /locus_tag="KPH93_001186"
     CDS             complement(6225..6977)
                     /locus_tag="KPH93_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1688017.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSKMFKALMPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDMWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKAFFKY"
     gene            complement(7095..7364)
                     /locus_tag="KPH93_001187"
     CDS             complement(7095..7364)
                     /locus_tag="KPH93_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688018.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKNHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7839..8933
                     /locus_tag="KPH93_001188"
     CDS             7839..8933
                     /locus_tag="KPH93_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG1688019.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSRYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKDYLESKDNKFKK
                     EILSVISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNSVGEENFPKLIKVDS
                     LREGSIHGVISMCLKETDYQYSLSNKKGGY"
     gene            8935..9885
                     /locus_tag="KPH93_001189"
     CDS             8935..9885
                     /locus_tag="KPH93_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG1688020.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKQT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFNCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAK
                     QGDKLAIEILRESSTELSKLAQVILSKISNPMKRDKSEINIVLSGGTLNKTVYAEMLM
                     NNLKIENKDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9878..11062
                     /locus_tag="KPH93_001190"
     CDS             9878..11062
                     /locus_tag="KPH93_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688021.1"
                     /translation="MDRKKVIVQIFTGGFKTKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYTGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMIEMNNMSKGVTDNEDTPFGITKYINGKYKFKNKLWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIYKNKEIAKRNFLEILGIKEYEGDVYPIEFVLEEIDFMTRSLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11075..12571
                     /locus_tag="KPH93_001191"
     CDS             11075..12571
                     /locus_tag="KPH93_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG1688022.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILAGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENVFL
                     GRELLKGSVFLNKKDMLEEYMKICEDFDFNIDPHIIVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKEGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLVEKKDYTFAKYEELPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEVEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12595..13545
                     /locus_tag="KPH93_001192"
     CDS             12595..13545
                     /locus_tag="KPH93_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688023.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVSALAGAITMKFATGGTNLFASMLLGIAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13569..14531
                     /locus_tag="KPH93_001193"
     CDS             13569..14531
                     /locus_tag="KPH93_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG1688024.1"
                     /translation="MKKLMKHLALLISFIMIFSLVGCSNNGSKGKEGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIIAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     AGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGIKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVKEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14897..15529
                     /locus_tag="KPH93_001194"
     CDS             14897..15529
                     /locus_tag="KPH93_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1688025.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15940..16032
                     /gene="kdpF"
                     /locus_tag="KPH93_001195"
     CDS             15940..16032
                     /gene="kdpF"
                     /locus_tag="KPH93_001195"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG1688026.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16073..17755
                     /gene="kdpA"
                     /locus_tag="KPH93_001196"
     CDS             16073..17755
                     /gene="kdpA"
                     /locus_tag="KPH93_001196"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG1688027.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNMTTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17780..19837
                     /gene="kdpB"
                     /locus_tag="KPH93_001197"
     CDS             17780..19837
                     /gene="kdpB"
                     /locus_tag="KPH93_001197"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG1688028.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19856..20482
                     /locus_tag="KPH93_001198"
     CDS             19856..20482
                     /locus_tag="KPH93_001198"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG1688029.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTSVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIKQFLKDNPTVKKEDIPTDLLTASGSGLDPNISPASAKIQIPAV
                     SKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20719..21627
                     /gene="cysK"
                     /locus_tag="KPH93_001199"
     CDS             20719..21627
                     /gene="cysK"
                     /locus_tag="KPH93_001199"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG1688030.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEKRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21654..22244
                     /gene="cysE"
                     /locus_tag="KPH93_001200"
     CDS             21654..22244
                     /gene="cysE"
                     /locus_tag="KPH93_001200"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG1688031.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVGYEYDELDNVYYI"
     gene            22405..22593
                     /locus_tag="KPH93_001201"
     CDS             22405..22593
                     /locus_tag="KPH93_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG1688032.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22647..23516)
                     /locus_tag="KPH93_001202"
     CDS             complement(22647..23516)
                     /locus_tag="KPH93_001202"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG1688033.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23713..24957)
                     /locus_tag="KPH93_001203"
     CDS             complement(23713..24957)
                     /locus_tag="KPH93_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688034.1"
                     /translation="MENNLNTAKRNNTELSQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     CDKSLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSMENNTNITIYENDTVQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYNKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFIEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9644 a         2698 c         4522 g         8234 t
ORIGIN      
        1 atttattcac aagtatatct tataaaatat gttataatta atagaaaatt gtcatataat
       61 tacttaagaa gtattattat aaataacatt gaaaggtagt ggttagaata tggataccca
      121 taataaatat gtaaatttta taaaaaatat acctgtacct tttttatatt gtagaattgt
      181 aaagagacaa gaagatatag aatatcgagt agaatatata agtaagggta tgggcaaagt
      241 attacaatta gaagaaggta tttgtgataa aaatatacta gatgtattgc ctgtatttaa
      301 gtctaaaaaa tatttcaaag agttgttctc aaatgaagtg gattgtataa aaaggtacat
      361 tccgacactt aaaaactgga taaatataaa aaaacagata ataggggatt catacataat
      421 tttgtatttt ggtaaaattg tatttgatta tagacagata atcgattcct ttgataaaaa
      481 ggaaaaagta gcttatatta aagacgaaga aggcatatat atagattgca gtgaaaattt
      541 aataccaata ttaaataaca acattaaaac aacgaaagat atctttggta aaaatgatat
      601 agaagtatgg ggagagaata caggaaaatt atttagagat gattatagag aaggagtatc
      661 tagtaaaaaa agatttctac aaaatctttt tgaatatgaa gaaacatttt ttatggttga
      721 aaaatatttt ttgtatgatg aagacgaact tttaggtact ataggtataa tagacaatat
      781 aatatatagt gattacagta atagaaacta taactcaaaa gatttaatga agatgataga
      841 gcactcaatt ccagagaata tgttttataa agatgtgtat ggaaattata ttggatttaa
      901 ttcaggtttt ttgaatttag cttgtatgaa taaagaagaa ttattaggga agaatagtta
      961 taaaatatca gaagaagaag ttttaataga taagatattt gaaagtgata agggtgtaat
     1021 tgaaaataaa aaagtagtta catttgagtt aaatgtgagt ataaatgatg aaaataagtg
     1081 tattgagatt acaaaaaggc cgttttttga tagctatgga agtgttattg gaataattgg
     1141 aacagcaagg gatataagta gaagaaagag attagaagag gaaatggata aaactaggat
     1201 ggaatttttt gcaaatttat cccatgaact tagaacacct ataaatttaa tatcatcttc
     1261 attacaagtg atagaaaaaa aagaagcaga tttaatagaa tcaaatgata ctttaaaaag
     1321 aaatttaggt ataataaaac aaaatggaaa tagaatactg aggttagtaa ataatgttat
     1381 agactttaca aaaatgcaat caggatattt agattttaaa ccagaagaat ctgatataat
     1441 tgcatttata gaggaaatat gtatgtctgt agctgatttc gcaagtcaaa ataatataca
     1501 acttacattt gatacagaaa tagaagaatt ttcaatgtta tttgattcag aaaaattgga
     1561 aagaataata ttaaatttac tttctaacgg aattaagtac aataaggaag atggaaaaat
     1621 aaacatattt ttatatgtga aagataatat atttaatatg aaaatatctg atagtggtat
     1681 aggtatacca aaagaaaaaa tagataaaat atttaatagg tttgaacaga ttgacaatga
     1741 actttcttac agagttaaag gaagtggcat tggactttca ttagtgaaat ctttggttga
     1801 gttgcatgag ggaagtatat ctttaaaaag tcaacttggt attgggagtg aatttatagt
     1861 ttcattacct gttagaagta aaaataatat tgaaaaatat aatcataaaa gagaaatcag
     1921 taatgaacta agtaaaaaat tagaaataga attttcggat ttataagttt aagtataaaa
     1981 ataaaatttt aaaaaagtat tgacaattga tttagaaggg tttaaaataa attacaacac
     2041 aataaataaa aaggcaatga ataggaagag taaatctaat tcgattacca gagagaagag
     2101 tccattggct ggaagacttt ttaaattaga gggattgaaa actacctagg agctactttc
     2161 tgaagtcatc tgcatcagta gattttagta agaatagtcg ttagctgcgt taaagtgtaa
     2221 agtgagtttt catgatatat gaaaactaaa ttaggtggaa ccgcgggaat aatactctcg
     2281 tccttatgtt ataaggatga gagttttttg ttatttaaaa atataaacag taatcataat
     2341 aattatagaa aatataaaag cgatgaatag gaagagtaaa tttaattcga ctatcagaga
     2401 ggaaagttca caggctgaaa gactttctaa gtttgagaaa ttgaaaacta cctaggagct
     2461 gtggtctgaa gttactttaa aaagttttag taagactaac cgttaactgc gttaaagtgt
     2521 aaagtggatt ttatcaataa atgataaaat caaattgggt ggaaccgcga gtatagactc
     2581 gtccctttat atagggatga gtctattttt attgcaataa aagattgaaa ataaaaaaaa
     2641 atcggaaaat tataaattta ggaggaaaat aaaatggaga ataaagtgaa aattggagta
     2701 ctaggatatg gagttgtagg aagtggactt atagatataa tagataataa taaagaaaaa
     2761 agaaatatag aaatagttgg aattttagtt aataatttgg aaaaacataa ggataaaaaa
     2821 tattctaata taataactaa taatatagat gatatattta ataaagatat agatatactt
     2881 gttgaagtca tgggtggttt agaaccatca ttatcttata taaaaaaagc attaaacaat
     2941 aaaattcatg ttgttacagc aaataaagat ttgttagctg aatgtggaga tgagcttgca
     3001 aaattagcaa gtgaaaataa agtaagtatt aaatttgaag cttcagttgc aggaggtata
     3061 ccagtattaa aaccaataat agaatcacta gaaggaaata atatagatag tataaatgca
     3121 attttaaatg gaactactaa ttttatatta tctaaaatgt atgatgagaa tctatcttat
     3181 gatatggcat taagacaagc tcaagaatta ggttttgctg aagcaaatcc agagtcagat
     3241 gtattagggt atgatgctgc tagaaaacta tcaatattat caactctagc ttatgacaat
     3301 agagtatatt ggaaagatgt gtatttagaa ggaattacag atatagatga aaaagatatt
     3361 gaatacgcaa aaaaattaaa ttgtaaaata aaattgattg gtcaaagtaa gtatgaaaat
     3421 gataaagtaa gtgcttttgt tagacctgtt ttagtagaaa aagataatat ccttgctaga
     3481 atagacaatg agttcaatgc tgtaatagtg aatggagatt cagtaggaga agtttctttt
     3541 gtaggtaaag gagctggaag tttagcaaca ggaagtgcgg tttattcaga tgttatagat
     3601 ataatagata atagagtttc aagtatagat tcttttacta aagataaaat acaagttaat
     3661 aaaatagtta gagaaaagtg tggagcactt cttagattta aaaaatgtaa taaagatgaa
     3721 attctgaata tagttgagaa ttgtttagta aaattcgata ttttaaatga tgatgatgaa
     3781 cttgctatta tggtttatgc tgattctgaa tatgaaataa acaacagttt atgtctaata
     3841 aaagataaag gatattgcga aaaaatgaat aaaatgttga aaataagtta aaaaagaaaa
     3901 aatataagtt tatatgtaac atattttgac gattttaata ctatatagta tgagatgaaa
     3961 caaaacatct cataaaaaaa ttaaaattaa agggagagat atgttatatg gaaaataaaa
     4021 aatgttattc agaagattgg tatgaaagag gagaatctac agctaaatgg ttccaaaatg
     4081 atagagaaga atatgaaaga gcagcatacg atgaagatag agaaagaaga ggttcaaact
     4141 gtggttgttc agattcagga gaaaatagac ctagaaattg tgaaagattt agaagagaag
     4201 ctgagataag agaaagagaa gcaagagaag cattctgtga atcttcagag aaaaagaaag
     4261 aagcattagc atatgaatgt gaagctagaa aattatggga agaagcagaa aaatactggg
     4321 atgaatattc aaaatacaac tatcaaggaa ttgaatattt agcagaagct gctagattat
     4381 ttgatgaagg tatggaatgt gaagctagaa gaaatggaaa taatggagga aacaataata
     4441 attgttgcca taaatgccat aaatgtaatt gtaactgctg tagaaaataa aaaaattcat
     4501 tgacaaatca atagttaaaa tatatcatat tatgtataat aaagagaaaa ttcagaaaaa
     4561 aataatgatt agaagaaata taagtatatt tattagacaa atagtgctca gttagtgagg
     4621 tgagtacaat atgattaaac tcccatcctc tagcttttga tagagggtgg gagtttttat
     4681 ttattgatat atcttaaatg aggataatat tgtaaaataa atgagtatga tattgtaaaa
     4741 ttaggaggta aaaaatgaag agtattaaaa catcaagtat gaaagaaaag gtagatgaaa
     4801 agatattaag agagcaagta agttctataa tagaagatat aagaaataat aaggatgttg
     4861 cacttaaaaa atataatgag aagtttgata gaaatacaag agatgaattt agaattacta
     4921 aggaagaaat aaaggaagct tataagcatg tagatgacga atttataaat aacttaaaga
     4981 ttgctgccaa aaatataaga gaatttgcta aagctcaaaa actaagtttt gaaaaccctt
     5041 ttgaaaaaga aatttatcca ggtgtaatct taggtcaaac taatattcca atagagtcat
     5101 gtcttgcata tgttccagga ggtcagtatc cattgttttc aacagctctt atgcttataa
     5161 tacctgcaaa agtagcagga gtaaaaagaa tagtggcatg ttcaccaact atgaaaaata
     5221 ctgaaaagat aaatcctaaa acattggtag ctatggatat agcaggtgct gatgaaatat
     5281 atgcaactgg aggagttcaa gctatagcag catttacata tggaacagaa aagattaatc
     5341 cagttgacat aattgtagga ccaggaaata agtttgtaac tgaagctaaa agacaatgtt
     5401 atggacaagt aggaattgat tttgtcgctg gaccaagtga agttcttata atagctgatg
     5461 aaacttcaaa tccagtttat attgcagctg atttattagc acaatgtgag catgatttaa
     5521 atgcaagagg aatattattg acaaatagct tagaaatagc acaagaagtt gaaaaaaata
     5581 tagaaagtat gttaaaagat ttgccaacaa aggacatagc atatagttca tgggaaaata
     5641 atggagagat aatactggtt gatgatatgg aagaagccat taaaataagt aatttttatg
     5701 caccagaaca tttagaagtt gcagtaaatg agtataatga tatttgtgat agattaacta
     5761 actatggttc tttatttatt ggaaatcttt ctgctgaggt atttggtgac tatgtctcag
     5821 gtaccaatca tacgttacca acacttaaag catcaagata tactggtggt gtctgggttg
     5881 gaacttttat caaaacttgt acaaagcaga tatttaatga aaaagctata caatcattag
     5941 cacctgtagc tgaaaagctg gcaaaagaag agggattata tgcacatgca aaggcagctg
     6001 aggttaggtt taaaaagtag taaaaaaatt ctatgttgtc agtcgagatt tagtattaga
     6061 ctgagataag gaaaacgccg agaacctgaa taaatactat attttgtggg tcatcggcgt
     6121 ttcttttata tctgtatatg ataggataat actacctgtg tgtttaaatg ctaacttaat
     6181 atttcttttc taaataatgt ttatgaaata gttaatattt ttagttaata cttgaaaaaa
     6241 gcttttgcta actgtgcaaa ttcgtctgca tttgtcaata tagcagggtg tcctccctga
     6301 ctaaaaatat gaatacttgc ttttgaaata aaagttgcca tttgtttgta ctcttcttca
     6361 agattatgac gacacatttc atcttcctta cttcctgtaa ataaaattgg catttctaat
     6421 ttacacaatt ctttatgaaa caaaggtcgt ttctcgtttg cacattgcaa tagagctttt
     6481 gtatcaagat taacaacatt ttcccaatct tttccctgac accattcata gaattttcgt
     6541 gcttgaatat cttgctttga tttttccctt ccagtgatta gattatctat gaagttatca
     6601 ttaagagtac gtccatcaaa gctatctgct ataattgctt caactaaatc gggacgttct
     6661 aacccagtat taacagctgc ccatgctcct ccacttgttc caattaagcc aactttacca
     6721 caattcaaat gttcaatcag tgctatcgtc tgtaaggctt catcatgcca catgtcagga
     6781 gaaaattgag aaactctatc cgactgtcca ttacctaaaa aatcaattaa gatacatcta
     6841 aagttttcta catataatgg cataagtgct ttaaacatct tagaagaacc agtattacca
     6901 tgcaggaaaa ttatcggttt accttgtcca tattctttat aaaaacaaga attaccttga
     6961 tactgaaaat atgacatata ttctcctcct ttttatttgt taagttaatt ataacaatat
     7021 aggtatgaat gaaaaacctc atgttctttc ttagaataaa aatgtgccac gcataaaacg
     7081 tgacacattt ctaattataa attttgctgt ttaagtattc tttgtatgct tttctctgtc
     7141 aaaaagtatt tctctgataa ctgctttata ctcattccat tttggaagtt tatacagatt
     7201 tgagaattac gagaagctaa gacttgtttg gtgtttgtgt tttcacccca atgatttttg
     7261 tgctcttgct ttttgggaat ataaatcact ttaccatcta tatattcttg cacttgctca
     7321 atcagttctt taggcagtaa gtcaattgaa ttttgatagc tcattttgca cctcctaaat
     7381 aattattctt taggattagc aaaggctact gccactcaca attaaaaata agatttttta
     7441 ttatgtgcaa tagcctttgc tatgcacaag ttccaattcc cataaactca atactccttt
     7501 ctggtttgaa aaattcatct gaacattgat ttcgttttta atatagagtt caatgacaaa
     7561 tttacatttt tattatatat attcaggtag ataattgcaa ctatctttgt atgagggtat
     7621 tttattatag tcaatactat aatatgtatt tatatcatca aactaatatg aacagttgaa
     7681 aggaatatga acaataaaac ttaatcagga agaataatta tgaagcagag aatatagaaa
     7741 tttgatttta aagttttatt gaaaatttat tgacaaattt aacttgtaaa tattatacta
     7801 aacttaataa ataaagttta taaagtaagg tgttgtctat gaaaaatata gttggaaaac
     7861 cgcaaacgat aaaaaaagta aatgaagatt taataaaaaa tattataaaa aatgagggtc
     7921 ctataacaaa accagaaatt tcaagcatta ctgaattgag tttagctaca gttaataaga
     7981 ttgtagagca gttagcttta aaaaatgagg ttaaagtaag tggactaagt gaatctactg
     8041 gaggaagaag agcgcaatta tttgaaataa atgcaaactt agaacacata atagctttat
     8101 attattatag aaattgttgt ataggggtag ttgccaattt attaggtgaa ataatttatc
     8161 aagaagaatt taatataaga atggatacat atgaaaacgt taaccaagat actttttttg
     8221 ttatagatac tttaataaaa aaggaaaaag gaaacaatat aagggctatt ggaattggtg
     8281 ttccaggagt tgtaaaaaaa ggtgtagtta aaaatatacc aactataaaa agttgggaag
     8341 gtataaattt agaaaattta ataaagagta gatacaatac taaagtgttt atagaaaatg
     8401 atacaaatat tactactatt gggatttatc aaaaacagta taaagacaaa tataaaaata
     8461 tggcccttat gtatttagaa gatggtattg gttcaggtat agttataaat ggtgagttat
     8521 atttaggttc tacaaatttt gctggagaat taagttatct aaatatagaa tttggtaagt
     8581 ataactctaa taacttaaat cttgaagatt atattatgag tttaagaaaa gattatttag
     8641 aaagtaaaga taataaattt aagaaagaaa ttttatctgt aatatcaaac tttataagga
     8701 ctcttgtatg tgtcctaaat ccacaagcta tgattataca gtgtagtata ttaaccaaga
     8761 gtgacatgga gtttatagaa aaagatgtaa gcaatagtgt tggagaagaa aattttccta
     8821 aattaataaa agtagattca ttgagagaag gcagtataca tggagtaatt agtatgtgtt
     8881 taaaagaaac tgactatcaa tattccttat ctaataaaaa aggaggatat taatgtgaaa
     8941 tatttggttt caatagatgg aggaggaaca aaaactaaat tttgtgtaag tgacttagat
     9001 ggaaacatac ttaaagaaca taccactggt tcaactaatt ataagtcagt tggaattaaa
     9061 caaacatatg aaaatattaa taatggattt aaaaaaatat taaaagattt atatatcgat
     9121 tatgacgata ttgagtacac tgtatttggt atatctggtt gtgattctcc taatgattat
     9181 aagattataa tggatgaaat attaaaaatt gggattaaca aagaaaaaat atatctggca
     9241 aatgatgcag ttttagcttt ttatgcacaa gctgattccc ctggtttagt aattgttgct
     9301 gggacaggtt caattatatt aggaattaaa gaagatggag aaatatatag agttggtgga
     9361 tgggggtata attttagtga tttgggttct ggatatgata tagggagaaa attattaaag
     9421 aaagtattac tttattgtga tgaatgtcat gagtatagtg atttatttaa ttgtgtctta
     9481 gatttctttg gagcaaatag ttttgaacaa cttgcatata tgattactga tataaacaat
     9541 aatgttgaaa tagcaaactt agcctcatta gtaattgatt gtgccaaaca aggtgataaa
     9601 ttggctattg aaatattaag agaatcatca actgaactgt ctaaattagc tcaagtaata
     9661 ttgagtaaaa tatctaatcc aatgaaaagg gataaaagcg aaataaatat agttttgtct
     9721 ggtggcacat taaataaaac tgtatatgct gaaatgttaa tgaataattt aaaaatagaa
     9781 aataaagata aaaatttaaa atttatatct caagaaaatc agccagtata tggggggata
     9841 agacttgcta tggcattagc caataggagg gtcaagcatg gatagaaaaa aggtaatagt
     9901 acaaattttt actggaggtt ttaagactaa agaaataaag tttaatgata taaaatgtaa
     9961 gctactagaa ttactggact gtattgatat agaaaaggtt atcatgggct ggagtgtaga
    10021 tgaagaatta tatgtagaaa ctaaaaaact attaaataaa tataatgttg aactatatct
    10081 ttggttgcca tgtttttcag aaataggatt acttgaaaaa agtaatttac taattgatta
    10141 tacggggaag gaagtcaagg gctatgcttt acaagaggat gaaaattttg aattttattg
    10201 cccaaataat aaggaaaata taaaaaatat aaagaaaata tatagtactt attttagtga
    10261 tattaaattt gatggaattt ttttagataa aataaggtat ggttcttttt cgaataaact
    10321 aaatggtgtt tttaattgtt tttgtaaaga ttgtttaaat atatatgaaa aaaataatat
    10381 tgataaaaag tcattaatga tagaaatgaa taatatgtct aagggagtta cagataatga
    10441 agatactcca tttggtataa caaaatacat taatggaaaa tacaaattta aaaataaact
    10501 atgggaagat ttttttgaaa caaaaaatca aaatatatac aaggcactgt tagagataag
    10561 tagttatttt aaaagtaaag gattgaaaat aggtatagat actttttctc cttttatttc
    10621 ttactttaca ggtcaagatt tagaaaaatt acaagaagta gcagatttta taaaaccaat
    10681 gatgtataga actacaaaag ctccagctgg tttgccattt gaatttgaaa ggtttattga
    10741 tgaatccata tataagaata aagagattgc aaaaagaaat ttcttagaga ttttaggaat
    10801 taaagagtat gaaggagatg tatatcctat agaatttgta ctagaggaaa tcgattttat
    10861 gacaagaagt cttaataaag atatatatgt aggagttgaa ataaataaaa aggaaattat
    10921 agctcctgtt acacctaaat acataagtga aaatatttca aatataaata agacaacagt
    10981 aaaaggatat gttttatctt gggatttgtt atcagcacca aaagaaaata ttgatgaaat
    11041 tataaaatgg tttcggaatt agtgaggtga tatagtgagt aagatattgg aaatgcaaca
    11101 aatatataaa agttttggaa aggtagatgt tttaaaaggg ataaattttg agctagaaaa
    11161 gggtgaagta agagctcttt tgggagctaa tggagcagga aaatctacac ttattaaaat
    11221 tttaggtgga gtacattctc aaactaaagg taaagtgatt ttggctggta atgaaataat
    11281 gtttaaaaat ccacatcatt ctaaagaaaa tgggattagt ataatttatc aagaattaag
    11341 cttgatacca acacttacag tacttgaaaa tgtttttctt ggtagagaac ttttaaaagg
    11401 aagtgtgttt ttaaacaaga aagacatgct tgaagaatat atgaaaattt gtgaggattt
    11461 tgattttaat atagacccac atataatagt ttctaattta agtgtagcaa accaacaaat
    11521 ggttgagata atgaaagctg tttcatgtaa ttctgaaatt atcataatgg atgaacctac
    11581 aacatcactt actaataatg aaaaagaggg gctatttaaa ataataagta gactaaagtc
    11641 taaaggtaaa acaatagtct atatttctca catattagag gaagttttta aagtatgtga
    11701 tagtgcaagt ataatgaaaa atggaattat gattggaact tatgatgtag aggatttaac
    11761 taaattaaag ataactcaac ttatgacagg aaacgataat cacaaacttg ttgaaaagaa
    11821 ggattataca tttgcaaagt atgaagagct tccagtacta gaagtaaaga atataagctg
    11881 gtctaacaaa ataaaaaacg tttcatttaa agtttataga ggagaagttg taggtcttgc
    11941 aggtcttgta ggttcaagaa gaactgaaat tataaatttg atatttggta tagataataa
    12001 agacagtgga gacattttta tcaatggtaa aaaagtagac ataacttctc ctaaggtggc
    12061 tataaaaaat aaaataggat tgattcctga agatagaaaa aatctaggat taattttaga
    12121 acaagctata tacacaaact catcatcagt acaaattgat aaatttaaga aattcttgtt
    12181 tatagataga aataaagaag ttgagttttc taaaaaaggc gttaaggaat taggaataaa
    12241 agttagtgat gtaaagcaag aagtaaaaga gcttagtggt ggaaatcaac aaaaagtagt
    12301 agtttctaag tggttagaga aagatttaga tttaataata tatgatgagc cgacaaaggg
    12361 aatagatatt gctgcgaaag aagatatctt caatacaatc aaagaatttt caagtaaagg
    12421 aataggagta atattcatat cttctgattt agaagaagta ataagagtaa gtgatagaat
    12481 tttagtaact agagaaggtt ctattgtaga tgatttaaaa aatgagaata tacaggttca
    12541 agacataatg aacagtatat ttaatgtata aaaaatgaaa aatggaggac tgagatgaag
    12601 aaagcttttg agaagttaaa tttaagagat tatggagtat tactaggttt tttagcgtta
    12661 tgtatagcta taagtattgc aactccatca tttttaggta aacaaaatat acttaatctt
    12721 ttacgacaat cttctataat tggaataatt tcagcaggta tgacttttgt tataatatct
    12781 ggaaactttg atatatcagt tggagcagta tcagctcttg caggtgctat aacaatgaaa
    12841 tttgcgactg gtggaactaa tttgtttgca tccatgctct taggtatagc aatttgtgca
    12901 gtaataggat taatcaatgg tatagtagtt gcaaagataa atgtaccatc attaatagct
    12961 actatggcta tggtcactat tgtaagagga ttgctgttga tgcttacagg cggatatcca
    13021 attactgaaa atataccaat acttgattac attggaaatg gatatttgtt taatatacca
    13081 ataccagtag taattttctt tatagtggtg ttagcttcgt ttattgtact taataaaact
    13141 aagtttggta gatatgttta ctctgtagga gggaatcaag aagcaagtaa acttaatggt
    13201 ataaatgtag attctcacaa agtgaaagta tttattatta atgctgtact tgcaggaata
    13261 gctggaatcg tactaacagg aagacttggg actgcaactg cgattgcagg tgaaggatat
    13321 gatatggatg caattgcatc tgtagtaata ggaggtacat cagtggcagg aggttctggt
    13381 tctgtactta aaaccgttat aggagtatta cttatgagtg ttataaacaa cagttttaat
    13441 cttcttggaa tagatgtata cttccagtac atatttaaag gattaataat tttagcagca
    13501 gttggatttg attcttatag caagaaaaag cttgcttcaa gataaaaaat aagaggggga
    13561 atttttttat gaaaaagtta atgaaacatt tagcattatt aatatcattt attatgatat
    13621 ttagcttagt tggatgttca aacaatggta gtaaaggaaa agagggagaa aagaaaatag
    13681 cagtattgct tcctggttca acaggttact ttgtagcaac taaacaaggt atagatgcaa
    13741 aatctaaaga attaggtgta agtgtggaat atgcagatgc tcaatgggat gctagtaaac
    13801 aattatcaca agctgaagat tttatggcta aaggggtaga catgattgca ttatgtggag
    13861 ttgattctgc tgtaagtgag agaatagtta aagctgccaa tgattcagac gttcctataa
    13921 tagcatttac aaattctata ggaagtaacc caactggaga atttaaaggt ttggttacat
    13981 atataggaca aaatgaggaa gagacaggag cattaactgg aaaaattgct aaaaacttat
    14041 taggagaagc tggtggaaaa gctgtattaa tagaaggagt accaggaact actccacaag
    14101 tattaagaaa aaaaggtctt gaaaaagaat taaaagatag taatatagaa atagtttata
    14161 atcaaacttc aaactgggag aaagagcaag ctttaaaagt aacagaagat ttaattcaaa
    14221 agaaaactga ttttaatgtt ataatatgtc aagatgacaa ctctgctaca ggtgcaggtc
    14281 aagctttaaa agatgctggt attaaggata aagttaaagt tataggtttg ggtggaagta
    14341 aagatggatt aaaagctata actgatgggt taatagatgg tacaacttat atgtctgctg
    14401 tgaaagaagg tggattagtt atagaaaagg catctaaatt ccttaaaggt gaaaaaatag
    14461 aacctgttac acaaattaaa caagttgaag ttaataagga taatatttca gagttcaagg
    14521 gtgaatggta aattgtcata gataataaat aagattttca gagttacttt agaaactata
    14581 taaagtaact cttttttaat ttaatgataa attattgaat agtatttcta aacaaataaa
    14641 aaattaatat aaacaaagac ttaattatac taaattataa gtcaaactaa tataaactta
    14701 tggaaaaaag gaaaacgtct aaagtattgg aattactgta gtgtaggtat ggtattaaaa
    14761 aaacataaaa aaacataaaa aaattgttga aaaaaatata gaaattttgt ataattaaag
    14821 atttgaaaag tgtggtatac aaagccaatt cttttgtata taatatatat aaattcagaa
    14881 aaaggaaggg atatgtatgg ccaggcggtt atttttagaa aggattggac ttatattttt
    14941 aggttcagca atattagcat ttggagttta taatttttat tatttaaaca acataacaga
    15001 aggtggagtg ttaggtattt tactactgtt aaaaaattta ttcaatatac aaccagcaat
    15061 agcaaatgtt gtaatagatg gtttactact tttagtagga tataaatttt tcggtaaaaa
    15121 attccttata tattcaatag tagcttctat aactttttca gtgttatatg acttatttga
    15181 agctatagga cctctagtac cacaatcaca aaatatgctt ttatcaacta ttttggcagg
    15241 tgtaactgtt ggaactggtg taggtatagt agtaaaagca ggttgtgcat caggaggaga
    15301 cgatgcttta gcattagtaa tttctaagac tacatctttg aacatagggc aagtttattt
    15361 agcaacagat gttattgtat tgttgttatc attattttat ctatcagcgt ttgatatctt
    15421 ttattctttg attgctgtta caattagtgg taaggtaatt gattttattt attatcatgg
    15481 taaaagtttg gatatggata taagtaatga tatagttcca gaatgttaat ataaattaaa
    15541 taactttaga ttttattagg ggtggcagat ttgccacctt ttttgtgtct aaatctttat
    15601 agaaagtata atcacatcta ggtttttgca aatctcaaat atagaattat gttggaataa
    15661 ctgggaatac gattgctaga tgtgcgtttt aaaaactcaa ttttatattt atgaaatact
    15721 aatcattaat ggatgtttct taatcctatc tttataccta aaatgataag ataaatgaag
    15781 tagcaataca aaactaaaat ttgtaaaaga gattaaagta aatggaggag ctaaaatgtt
    15841 ggatatagtt atgatagcat tgttattcat aggatttgtt tccatgaagt tacttgctga
    15901 ttggtgtggg aaacaaactg aaaaaaaata atgtaatgta tggagggatt acctatgtgg
    15961 tttttggcag ttgtaattat aatgttaata atatatttgg tatatgcgct tattaatcct
    16021 gaaaaatttt aaacagtata aggctaataa gtaagttgaa ttggggggaa caatttttat
    16081 ggtgcagata gtaatagtgt tagcgatttt tatgatttta gtaataccta tgggcaagta
    16141 tttatatcat atagctacta atcaaaaaac atttggagat agactatttg ataaagtaga
    16201 caattttata tataaggtat gtagtataga taagaaaaaa gaaatgaact ggaaacagta
    16261 tgccttagct ttgttattta caaatgcagt tatggtattt ataggatata tcatacttag
    16321 aactcaaagt atgcatattt ttaatccaag tggaattaaa agtatggaac aaggactatc
    16381 atttaataca ataataagtt ttatgacaaa tacaaatctt caacattatt caggtgaatc
    16441 aggactttca tactttagtc agatgacagt gataatatat atgatgttta catcagcagc
    16501 aacaggatat gcggctgcta tggcatttgt aagaggtctg gtcggtaaaa agaaaacatt
    16561 gggaaatttt tatgtagatt taattaggat aacaacaaga gtacttttgc caggagcttt
    16621 aattattggt cttatattag ttacacaagg agtaccacaa acttttgctg ggacagaaac
    16681 agtgactact atagaaggaa agcttcaaga tatagctaga ggtcctgtgg cggctcttga
    16741 atcaataaaa catttaggaa caaatggtgg tggattcttt ggttcaaatt catcacaccc
    16801 atttgaaaat ccaacaataa tatcaaatat agtagaaata ttatctatga tgatattacc
    16861 aggagcttgt gttgttgcat ttggtcacat gataaagaat aaaaaacaag gttgggttgt
    16921 gtttggcgca atgtcaatca tatttttaat aggtctagta gtttgtttta aagcagaaag
    16981 tgcgggaaac cctattttat cacaattagg gcttaatcaa agtatgggaa gtatggaagg
    17041 taaggaagtt agatttggta tagcacaatc atcattattc acaacagtga ctacttcctt
    17101 tacgacaggt acagttaata atatgcatga tacactgact cctttaggag gattagtacc
    17161 acttttaaat atgatgctaa acgtagtatt tggtggtaaa ggcgtaggtc tgatgaatat
    17221 gttgatgtac gctataatag cagtattctt atgtggctta atggtaggaa gaacaccaga
    17281 gtttttaact aagaaaatcg aaggaaaaga aatgaagctt attgctttac ttattatatt
    17341 acatccactt ttaatactaa tgttctctgg tctttcagta gcaataccag cagggcttga
    17401 aggaatttca aatcctggat tccatggatt atcacaggta ttgtatgagt ttgcatcttc
    17461 agcagccaat aatggttcag gatttgaagg tctaggagat aacactatgt tttggaatat
    17521 gacaacaggt atagttatgt tctttggaag atatgtgtct attatagtac tgctagcaat
    17581 atcaagtttg ttagcatcta aaaaagctgt aaatgaaagt ataggaacac ttagaacgga
    17641 taattttaca tttacaatag ttttagtact tgttgtttta attgttggtg ctttaacatt
    17701 tttcccagcc ttagcgcttg gacctatttc ggagcatcta gtgttgtggc actaaaaaat
    17761 tgggagggag taaagtttta tggaaactaa aaaaacaaag tttattacaa aagatatttt
    17821 caaaacttcc attatagaag cttttaaaaa attaaatcct aaatatatga tgaaaaatcc
    17881 agttatgttt gttgtagaaa ttggatttat agtaacattg atacttacaa tagtacctag
    17941 tttgtttggt gatgaaggca ataacttaag agcttataat gggattgtaa caataatctt
    18001 atttataaca gtcctttttg caaattttgc tgaagctgta gctgaaggaa gaggtaaagc
    18061 tcaagcagat acacttaaga aaactagaaa agatacagta gcaagggtta tagatacgtt
    18121 aggaaatgaa aaaatgataa gtgcaagtga gctaaaactt ggtgatattg ttttagtaaa
    18181 tgctggagaa gttatcccaa atgatggaga agttatagag ggtatagctt ctgtggatga
    18241 gtcagcaata actggagaat ctgcacctgt catgagagaa tcaggtggag atttcgcatc
    18301 tgttacaggt ggtactacag ttgtaagtga ctggttaaag ataagaataa caacaaaacc
    18361 aggagaatcg ttcttagata aaatgatagc tcttgtagaa ggtgcttcaa gacaaaaaac
    18421 gccaaatgag attgctttaa atactttgtt agtagggtta acacttatat ttatggttgt
    18481 tttaatatct ctttatccaa tggcaaacta tgcaggtgta aagattccaa tttctactat
    18541 gatagctctt ttggtatgtt taataccaac tactataggt ggattgttgt cagctattgg
    18601 tattgcaggt atggatagag taactagatt taatgtaatt gcaatgtcag gaaaagcagt
    18661 agaagcttgt ggagacgttg atactatgat tttggataag acaggtacga ttacttatgg
    18721 gaatagactt gcatcagaat ttatagcagt tggaaatgca gattcaaaag acttaataaa
    18781 ttattctgtt atgtgttcat taaaagatga tacaccagaa gggaaatcta tagttgattt
    18841 aggtatgaaa ttaggttcaa caggtaagac aaaagaggca gaggaagctg agtttgtaga
    18901 attttctgca caaactagaa tgagtggtat taacttagct gatggtagag ctataagaaa
    18961 aggtgcttat gatagcataa taaaaagagt aaaagaatct ggtggtacag tgccagaaga
    19021 tttagaagaa aatgtaaatc gagtagctaa actaggaggg acacctttag tagtgtgtgt
    19081 agataatgag atatatggaa ttatctactt aaaagataca gtaaaatcag ggcttgtaga
    19141 aagatttgct agacttcgag agataggaat aaagacaata atgtgtacag gggataatcc
    19201 cttgacagca gctacaattg caagagaagc tggagtagat ggatttattg cagaatgtaa
    19261 accagaagat aaaatagatg caataaaaaa agaacaatta gagggtaaaa tagttgcaat
    19321 gacaggtgat ggaacaaatg atgctcctgc acttgctcag gcagatgtag gtcttgcaat
    19381 gaatagtggt acaacatctg caaaagaagc agcaaatatg gtggatttag attcagaccc
    19441 aactaagata ttggaagttg ttgaaatagg aaaacagctt ttaataactc gtggggcttt
    19501 gacaacattt agtatagcaa atgatgttgc aaaatatttt gcgattatac cagctatatt
    19561 tactcttgca atacctcaaa tggagatatt aaatgtaatg aaactttcta catcatttag
    19621 tgcgatatta tcagctttaa tatttaatgc tataattatc ccatgtttaa tacctcttgc
    19681 aatgaagggc gtaaaatata gacctatgaa atcacaagct atgttaatga gaaatatgct
    19741 tatatttggt cttggtggag ttatagtacc atttttagga ataaaattaa ttgatttatt
    19801 gattacacct ttagtaatgg ttcttaattt aggttaataa atggaggata taaaaatgaa
    19861 aacattaaag ccagcattac ttgtaagtat tgtattgtta gttgtttgtg gacttgtata
    19921 tccacttgtt ttgacaagtg taagtcaagt tgcatttaaa gataaagcta atggaagtat
    19981 gatagaagta aatggtgtaa aagtaggttc agaacttatt ggtcagagtt ttacagatgc
    20041 gagatttttt aagggaagag tatcatcagt aaactataat acttatacaa aagaagattt
    20101 agtaccagat aaagatggaa atacatctta tggaggtgta tcatcaggtt cttttaatta
    20161 tggagctaca aatccagaac ttcatgatag agtacaaaaa gatataaaac agtttttaaa
    20221 ggataatcca actgtcaaaa aagaggatat accaacagat ttgcttacag catcaggttc
    20281 agggcttgac cctaatataa gtccagcttc tgcaaaaatt cagatacctg ctgtatcaaa
    20341 agcatcagga atatcagaaa gtaaattaca aaaaattgta gatgataata cttccaaaaa
    20401 actttttgga gttttaggtg aagatagagt taatgtgcta aaggttaatg tagaggtcgc
    20461 aaaaatatta ggtcttatat agtattttgt agaattttag taacaaaata ttacgcaaga
    20521 tttaatttaa agattaaatt ttaaagagtg aaaatcgata atgattttca cttttttatt
    20581 tgaatattac taatatttgg aataaggtga ttgtaagtat aaaatccaaa aaaaggttgc
    20641 attatcaaaa tgataatact ataatatatt tatcgttatc aatttgataa tagatttgga
    20701 catataggag gaaatagtat gttatataat aacgcattag agttgatagg aaacacacct
    20761 gtagtaaggc tgaataattt aggctgtact aatgggtgtt ctaacatata tgtaaaatta
    20821 gaaaaagtaa atccagcagg aagtataaaa gatagagctg tttatgaaat gttagagggc
    20881 ttagagaaaa gaggagaatt aaaaaaggga gatgtactag ttgaagcaac tagtggaaac
    20941 actggtattg cattatctat gataggaaaa ttaaaaggat atgaagtagt aatcgtaatg
    21001 cccgaaacga tgagcgttga gagaagagat ttaatgaagg cttatggggc aaatttggtg
    21061 cttacagatg gtaaagctgg aatggcaggt tccatagaaa aagcaaatga acttttaaaa
    21121 gaaaatccaa actataaaag cttaaaacaa tttgaaaata aagataatcc aaatgcacat
    21181 tataaaacaa cagcagttga aattatgaat gatgtcaaag atttagatat attcgtatgt
    21241 ggagttggaa caggtggtac tttaatagga actgcgagat atttaaaaga acaaaatcca
    21301 aatataagag taattgcact tgaaccaaaa aaatctccag ctatatcaga aaacaagact
    21361 ggacctcata aaatacaagg aataggtgct aactttgttc ctgaaaatta tgattctagt
    21421 gtagttgatg aagtaatatt agtagatgat gaagatgcat ttgatacagt taaattactt
    21481 gcagcaaaag agggaatttt agtaggtata tcttctggag caaacatatt tggtgctata
    21541 gagatggcaa aaaagtatcc agataaaaag atagttacag tagctccaga tggagttgat
    21601 aagtatatgt ctatgggaat attttaataa ttttatttga gggagtgaat actttgttta
    21661 agaaaataaa taaagatatt gaatatataa tgaagaatga tccagcagct aggtctaaga
    21721 tagaagtttt tttactttat ccatcagttc atgcaatgat aatgcataga atggctcatg
    21781 ctctttataa gaagaagaag ctttttacag caagattaat atcccagata tccagattta
    21841 tgactggtat agaaatacat cctggtgcta aaatggggga aggtatattg atagaccatg
    21901 gaatgggtgt tgtaattgga gagacagctg aagtaggtaa tagggtgaca atatatcaag
    21961 gagctacttt aggagctaca ggaaaagata ctggaaagag acatccaact gttggtgatg
    22021 atgtattaat tggtgctgga acaaaaatac taggaccttt aaatataggt tcaaattcaa
    22081 aaattggtgc aaattctgtt gtcgttaagg atgtacctaa tggtgcaaca gttgttggta
    22141 taccagcaaa aatagtaaaa ataagaaact tagaacctgt aaaaaagaat aaaaaagaag
    22201 taggctatga gtatgatgaa ttagataatg tatattatat ataactttag cataactata
    22261 aaataaactt tataaaaatt atttttaaaa ggtatttttt tcaaatttta ggacataata
    22321 aaaaagaact gaatgttaca gttttagttc atcaatcttt gataaacaag attttaaatt
    22381 tgtagcgtct aggaggattt tattatgaaa gcaaatgtta atcaagatac ttgtataggt
    22441 tgtggattat gtccatcaat atgtccagaa gtatttgata tgaaggatga tgggaaatca
    22501 catgttatag ttgatgaagt accaagtgat gctgaagaat cagcagcaga agctagagaa
    22561 agctgtccag ttgatgcaat agatgtgcat taataaaata aatgcctata aaaattgaat
    22621 tttcaatttt tataggcatt tgtcttttat tcagttagta tttcaacacc tgtttctgta
    22681 actaaaacca tacattccca ttgagcagat aatttaccat cttttgtata aatagtccaa
    22741 ttattttctt catcaacgaa aatttcgtga gttccttcat ttaccatagg ttctattgtg
    22801 aatatcatcc caggaaccaa tagcatctca gtcccttttt tagatacata acttacaaaa
    22861 gggtcttcat gaaagcttaa tccaactcca tgaccaccta tttctctaac aacagagtat
    22921 ccatttttct ttgcataaga gttgatagca tcacctatat ctcctaagaa tccccatggt
    22981 ttggcaactt caagaccttt ttggacacat tcctttgtaa cttgaactaa tttttttgca
    23041 ttttcgttaa cattacctat cataaacatt cttgaagcgt ctgaatagta tccattaaaa
    23101 atagtagaaa catctacatt tataatatct ccatctttta atataatatc tttacttgga
    23161 ataccatgac aaacttcatt atttactgat gtacatacac ttttaggaaa tccattatag
    23221 tctagtggag caggaatagc tccattttgt agtgtaaagt catgaaccaa cttatttata
    23281 tcttctgtag acattccaat ttcgatttta tctgaaatat aatccaaaat ttttgtgttt
    23341 attacagcac ttttttttat ttcctcaatt tgactttcat tttttataat atcatgagtt
    23401 ggtactatat gacctaactt agcaaatgaa tctattttgt tatccatatc catgtgacac
    23461 tttttatatt ttttagtact tccacaccaa caactgtcat ttctatttaa cttcataact
    23521 tttcatcctt cttttattat ttatttaaat tatgggctac ataaactatt ttaaaacata
    23581 accattataa atattataac ataaatatta agaaatcctt tatagatagt gatttattca
    23641 aagtataaat tattatatgg ttattgttga tgaaaagagg atagttaaat gtattattgg
    23701 atattagcgc acttaatagt ttagttcaag tttttcaaga aaatcatatg tcagaggaca
    23761 attttccttt aaagtatttt ttgaatcttc atcaagataa taatgtacaa atgattctgc
    23821 aaaaaattct tctataaact tgcttatata tgcatgtgca acttgtttag ggtcataaag
    23881 cttatatctt tcctgagcaa atacttctat aaattcttca gaatttgatt ttttatttaa
    23941 aacgttttta tctatagcat gtgcagtttc atgtaattct aaattttttg atgaatgtac
    24001 atttgcatag ctagggttac tataacctat tttagcaact atagaattat ttctatatat
    24061 accaggtaca tctttccaag tcttgcctga tcttacccaa catggaggta attgatattt
    24121 taaatcacta aattctggtt catcagttag atttgaattt attaatttta tttttatatc
    24181 cttttcttta agtgtctgta aatatttaat atttattttt gcaagtctgc ttttcatatt
    24241 ttcaacttca tctttattat aattcaagtc atcaggaagt ataagcattt gttcaattat
    24301 atctatgcta ctgtctttta tatcttcaga ttcttcatca aatttatatt gaacagtatc
    24361 attttcatag atagttatat ttgtattatt ttccatagaa taagagtgaa tatcttctaa
    24421 atctaaaata ttcatttgtt gtaaaatatg ttttgtttcg ccactccata ctactaaaga
    24481 aagtgaatta aaattaatta gtggattttc ttttgctttt atatcattgc ttatatctac
    24541 atcaaataca tattcaattg agtcctctat gttaagacta tctgtattct ttttaatata
    24601 taaatacgct tgttttaagt ctatataaat ctcatctaaa gatttgtcac aaaattctga
    24661 taaattatta gtaaagagtt cttcacttaa aagttcattt ttatcagtta tactttcaat
    24721 taaaatatta atttctttta tcatatcgta aatacttatt tttctatttg ctttttcact
    24781 agatacattg catggcgcaa gagcaatgca ataaaaatct ttttcttttt tataaaaggc
    24841 tttaagtgat tttgataaaa gtgtaaagtc tacacctatc tgtaagttga ccttaagtga
    24901 gtcattttgt gataattcag tattatttct tttagctgta tttaaattgt tttccataat
    24961 cattgccccc tttttattag ggtatcataa tttttttata tgtgtcaatt tatttttttg
    25021 aaaattataa tttttttgaa aattttattt tttataattt gtgaattttt tattatagaa
    25081 ataaagatgt tattatta
//
LOCUS       DAEMAE010000037        24835 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEMAE010000037 DAEMAE010000000
VERSION     DAEMAE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.037"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..732)
                     /locus_tag="KPH93_001204"
     CDS             complement(<1..732)
                     /locus_tag="KPH93_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG1688035.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKIKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALILGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPAI
                     STVTIGGAIYNLVAVTLGNIVGGALF"
     gene            complement(789..1760)
                     /gene="asrC"
                     /locus_tag="KPH93_001205"
     CDS             complement(789..1760)
                     /gene="asrC"
                     /locus_tag="KPH93_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG1688036.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSDKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(1773..2564)
                     /gene="asrB"
                     /locus_tag="KPH93_001206"
     CDS             complement(1773..2564)
                     /gene="asrB"
                     /locus_tag="KPH93_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG1688037.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCDTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEHFYNNKEKCESFKLIVGFKSPKDVLFTDDLKRWSEKLDI
                     LVTVDGADEGYSGNVGLVTKYIPELDIKDINNTSIIVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(2565..3572)
                     /gene="asrA"
                     /locus_tag="KPH93_001207"
     CDS             complement(2565..3572)
                     /gene="asrA"
                     /locus_tag="KPH93_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG1688038.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNTFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLENKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     V"
     gene            complement(3963..4652)
                     /locus_tag="KPH93_001208"
     CDS             complement(3963..4652)
                     /locus_tag="KPH93_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1688039.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEVFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELLKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(4715..5563)
                     /locus_tag="KPH93_001209"
     CDS             complement(4715..5563)
                     /locus_tag="KPH93_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1688040.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLAKANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(5582..6469)
                     /locus_tag="KPH93_001210"
     CDS             complement(5582..6469)
                     /locus_tag="KPH93_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG1688041.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGLIIDKSSMDTEAKEKGGEGIL
                     AKIKHIVKKYREENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(6526..7329)
                     /locus_tag="KPH93_001211"
     CDS             complement(6526..7329)
                     /locus_tag="KPH93_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688042.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESVIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWYLNDTIEKF
                     NAGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDKGLTIDDVHYTQE
                     GLNILGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(7489..7941)
                     /locus_tag="KPH93_001212"
     CDS             complement(7489..7941)
                     /locus_tag="KPH93_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG1688043.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGCVELRKGDFLVCYPEDAHMTGIRVLEKENIKKAIFKVLVR"
     gene            complement(8071..9573)
                     /locus_tag="KPH93_001213"
     CDS             complement(8071..9573)
                     /locus_tag="KPH93_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG1688044.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(9606..10472)
                     /locus_tag="KPH93_001214"
     CDS             complement(9606..10472)
                     /locus_tag="KPH93_001214"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG1688045.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(10557..11228)
                     /locus_tag="KPH93_001215"
     CDS             complement(10557..11228)
                     /locus_tag="KPH93_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG1688046.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(11501..11992)
                     /locus_tag="KPH93_001216"
     CDS             complement(11501..11992)
                     /locus_tag="KPH93_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1688047.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIVCGYCENEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(12014..13378)
                     /locus_tag="KPH93_001217"
     CDS             complement(12014..13378)
                     /locus_tag="KPH93_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688048.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKESNTKDNYCKFCGNELYEITSLRPLETKLDLKSKIKDISYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLILAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFSILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNIDSIKPIIVFSIYY
                     AFLMGMLALFSTFILDSNINFFNTTSYGATLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            13797..14972
                     /locus_tag="KPH93_001218"
     CDS             13797..14972
                     /locus_tag="KPH93_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1688049.1"
                     /translation="MNLEKIKKFFGFPLDKKHKKNVEKELCNYLHHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCYTFYIN
                     LWIIYVTFFCLWGCVITLFDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(15145..16542)
                     /gene="asnS"
                     /locus_tag="KPH93_001219"
     CDS             complement(15145..16542)
                     /gene="asnS"
                     /locus_tag="KPH93_001219"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG1688050.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(16619..16852)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(17021..17215)
                     /locus_tag="KPH93_001220"
     CDS             complement(17021..17215)
                     /locus_tag="KPH93_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG1688051.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(17489..19162)
                     /locus_tag="KPH93_001221"
     CDS             complement(17489..19162)
                     /locus_tag="KPH93_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG1688052.1"
                     /translation="MEKSYCIIYQGNIESALQENGINKYMVLNSQLAVIYVPIDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGSSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRVNSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSGAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFNSVGEVQDVIIQDGDDYALDITLNTNGPDKIGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     IGGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGAYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(19172..22570)
                     /locus_tag="KPH93_001222"
     CDS             complement(19172..22570)
                     /locus_tag="KPH93_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG1688053.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMGQNFFKISDSLGVLFIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEEEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGVSNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(22737..23933)
                     /locus_tag="KPH93_001223"
     CDS             complement(22737..23933)
                     /locus_tag="KPH93_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG1688054.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQGEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(23954..24775)
                     /locus_tag="KPH93_001224"
     CDS             complement(23954..24775)
                     /locus_tag="KPH93_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG1688055.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDNISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         7983 a         4498 c         2592 g         9762 t
ORIGIN      
        1 aaataaagct cctccaacaa tatttccaag tgttacagct actaaattat aaatagctcc
       61 tccaatagta actgtactta ttgctggtga aaataatgaa acactataaa ttgtcatatt
      121 tgctacacta tgctcaaaac ctgatgttat aaaagcaaat aaacataaga atatcataat
      181 tatttttgca gtatcactat tagttctaaa tgaacacaac actgatacac atactagaat
      241 attacataaa attcctctaa agaaaagagc tgtaaatggc atggatgctt tactagctgc
      301 tgtatttgca aaaaattcag ctacagggcc tttgtctact agaccagttc ctacaaatat
      361 aagacctaaa atcaaagctc cgattaaatt acctacccaa ctgtatgccc atattttaga
      421 agtatctttt atacttacac ctttatttaa cataccagca gacataacca tattgtttcc
      481 agtaaataac tctgttcccg tcattataac caaacttagt gctattgcaa atgataaccc
      541 cattactatc ttagtcatag gtgaacctgc atcagtcaaa agtcctccaa tagtaaatat
      601 caaaagtata cctataccta cataaagtcc tgcaaacgca gaagatacca aatattttat
      661 tttacttgtg tttaataaat ttatcttatt tatagctgca ttagttaatt tatctaaagt
      721 ttctttatgc ataattccct cttccttcta ttaaatttta atttattcaa atatatgttt
      781 tactcaattt atagatattt aatcccactc cagtatatgt tttcgtacat tttagttatt
      841 tctggaagct ctacaccttc aagtgcccat tttttaaatt ccataaatcc tgttctatca
      901 attatatatc ctatatgttc ttttcctcct ggtgcatcct tatctatata cttttctacg
      961 tatctataag tattaagtat tatctttatt atgctgtcct catctaccca taaaagaaaa
     1021 tcttctgcta gtctaggatt tttctttcct gttctaccca ttatagctaa tctatagtat
     1081 tttttctcat ctctagtcca tgcatttgtt gggcaattta gtacacactc tccacagccg
     1141 atacacttat ctttatctct taccacctta ttattttctg tttttaatgc tccaacagat
     1201 attttagaac atttttttac acatgctcca caatttacac atctatcttt ttcatatatt
     1261 ggtaaagcca taccaataat tccaaagtca ttcattctag cttttataca atcatttgga
     1321 caaccagtta aagccacttt aaaatgtaaa tcatttggaa atatagcttt ttctattctt
     1381 ttagcaaatt cagttgtatt atattgagcc tttggacaga ctttatttcc tatacacgct
     1441 gctatatttc ttgtacctgc tgctggataa cctgcatttt tatcactttg atttatgttc
     1501 attttttcaa taacaggttg tataatttta tttatttctt ccatatcttc catatttatt
     1561 cctaatattt caaacccctg tcttgtagtt atgtgtacct gtccattgcc gtactcactt
     1621 gcaattttag aaactatagc taaactctca gggtcaataa gaccaccagg tactctaact
     1681 cttaaagaag tctcatattt tgtttttgta attctataag catttttcat tactttttta
     1741 gtatttaaat ctcttatcac aaacttcgcc ccctaatcaa taagattttt agcatatgaa
     1801 taatcaaata ctggtccatc taaacatata taagtatcat ccattttgca atgtccacat
     1861 ttacctattc cacaacacat ttttctttca taagaaaccc aaatgttctt ttcatctaaa
     1921 tctcttttta aaaattcttc tactgtaaat ttcatcatca ttggaggtcc aactacaatt
     1981 atagaagtat tatttatatc ttttatatcc aattctggta tgtattttgt tactaatcct
     2041 acgtttccac tatatccttc atctgctcca tctacagtaa ctaaaatatc aagtttttca
     2101 ctccatcttt ttaaatcgtc tgtaaataaa acatcttttg gtgatttaaa tcctactatt
     2161 aatttaaaac tttcacattt ttctttatta ttataaaaat gttctactat tcctcttaca
     2221 ggggctaaag cacttcctcc tgctactact acaatttctc ttccttcata aagagataca
     2281 tcaaatccat ttccataagg acctcttata aaaaaagaat ctccttcttt ataattaaac
     2341 aactcgctag ttacttttcc tacatttctt attgtaaagt caatataatc ctctccatat
     2401 cctgatactg atattggact ttcaccgtat ttaggtatag atatctcata aaactttcca
     2461 ggcaatacac cttttgtatc gcagtttact ctaaaagtcc attctttatc agtatgttta
     2521 actatatcta gtatttttgc acttactggt atatatgggt tcatttatac ttcctcctta
     2581 ctagtgacta aatcatttat tttttctata cactttgaaa atgatatgta ttgtggacag
     2641 gcatcatcac atcttccaca accaacacac atatgatatc cgaatctttt tttaaaatca
     2701 tgtattttat gcatagtttt aaatctcatt ctttcacctt gtttttttct aaaagaattt
     2761 ccacctgcta tatctgtata tccatctact tgacaagaag cccaaactct tcttctctca
     2821 cctacatttt cgttttcctt atagaaaata tcctgcattg taaaacatga acatgttgga
     2881 catacaaaat tacatctacc gcatcctata catcttgtat catattcatc ccatattgaa
     2941 tttcctatca tttcatttga atcaatattc tctggcacat ctacagatat taaattatca
     3001 gttacataat caacattaaa ttcagttgct tctccattaa atacttcaaa ttcactatcc
     3061 tttatatcta aataaagtgt atctccttct atatttagtc caattgaata attatcagtt
     3121 ttgttagaat ccatactcac acaaaaacag tttctaaaac tttccttaca ccctacaagt
     3181 gcaaacttta ctttatccct gatattttta taaaaataat ctttttcttc gccattattt
     3241 agatatattt cgtcaagtct ttttatacca tttatgtcac aagctcttaa gaaaattaaa
     3301 attttcttgt tctctaaatc gctttcctta aactcttttt cagtaaaata aaacaatact
     3361 tgatttatgg gaagtacaac ttcttttggt gaaaaatttg ctttcttagc aaattccatt
     3421 tcttcaaaag tgtttacctc cttgtatctt ataacatcag tatctgaaaa agtaccttta
     3481 aatggtatta aaactggtgc aaatattttg tagtcttttt ttagataatt cagaccttca
     3541 ttgaacttag atgaatctaa ttttagtttc atttttatca cctttcttat attttagttt
     3601 tacttacttg ctatcttgtt taaatcttaa cactttattt tctatgcttc cgtgataatt
     3661 atcacagttt caaaaataat aaaaaaaata ttaatggagt ttattaatct ctgttaatat
     3721 ttttttatta tatatattat tcgatttcaa atccaatcac ttaaatacaa tcattaaata
     3781 caataaaact attatctact caattttttg aataataaat aatcttattt gcttttttat
     3841 acaatttaat agggtaaaat aaagattaat aaattagaca ataaaaaaga atctataaat
     3901 agattctcct aataataatt ttatttaatt aaaattctaa caacactctt acttaatcat
     3961 aattaattat ttttaaaata ccttgataat tcagtttctt taacataaaa acctttatta
     4021 tcaatcttaa gaagttcttc tttttgcaat acttttatca ttctagaaac agtctccctt
     4081 ttacagccaa gcatatcagc taaatatgta actgtaatat ttacatttag taaagtccat
     4141 tctccacaac aaactccaaa ttctctactt aacctatata attttgctgc aagtttttta
     4201 tctactttta tggatataga atttttcaac tgtctatata aacgtcttat tcttctttcc
     4261 atataagaaa taatatcttt tgtaagttga aaatcatttt gcataatttc taaaaattct
     4321 tcaatactat aataagctac aattgaattt tcaaatactt cacagccaac agtagagttt
     4381 ttagaagagt caaatgtaat atcatttatg acttccccac tatttaatat gaaaattact
     4441 tttctctctc cattttcact tatcttaaaa atagatacct ttccttctaa aagtatataa
     4501 acactgtcta agctatcttt ttcataaaat aaaatttctt ttttttgtaa tctttttagt
     4561 ttagcttttt ctttaagttg attcttcgtt ttagtactta aattattaaa aattattact
     4621 ttatcaatat tataatcttt taatttagac atgtaatatc tctccattcc taaattttta
     4681 agagagctct ataaaacatt acaaatttct tattttattc atttttaaac aattttatag
     4741 catttgtagt tttaattttc ctttcaatag cttcattaga aagttctttt actacctgtg
     4801 tatatactaa atcaataatg aaaaattgtg ctaatttaga agatattgaa cctgtttcca
     4861 gtactgtttc tcctgataca taagataagt gtacatctga aatatccttt aattcagaat
     4921 ttttattttc agtaattgat ataattttag cattattggc ttttgcaagc tttacagttt
     4981 ttataatctc ctctgtttca ccagaatgtg aaattgcaat tattaaatct ccttcttcca
     5041 ttatggaaga catcattatc atcgtatggc tactgtcaaa acttacacaa ttaagaccta
     5101 ttctcataaa tttatagttt gaatcctgtg ctattatacc agaatatcca atacctatga
     5161 agtaaacttt ctttgcattt attataagcc ttgcacagtc atgtacatcc ttactattga
     5221 ttatctctac tgtgttctct aaagtggtta catttgaact taaaagtttt tttgctgtat
     5281 ccaaagctgg ctcatcattt tgtatatttt tatttataat atttttttta ttgatagttg
     5341 atatttcttg tgctaaagta actttaaaat cctgtaatcc attaaaattc atttttttta
     5401 caaacctagt tatagttgct tcaccaatat tgctctcttt agctatttgt gatattggtt
     5461 tataaaatac atcatctata ttttctttta tataagctat taataattta tctgattttg
     5521 tgactttaaa agttggtgat tccaattgtt ctaaaatact cattaaaaac ccttctattc
     5581 tttatatttt gtttaaaaaa ttatataatg ctccaagcat tcctgcatca ttttgtctct
     5641 ttgcaaattc tatattagtg tttttgtaaa ctctctctat aagaacctcc tttaatgctt
     5701 tgtctatttt tggttttagg aacttttctt gtgccataat cccaccacct aagattacca
     5761 cttctggatt tattacataa catatatttg cgattccaac tgctaatgat tttatcatat
     5821 tatcgagttc ttttaaacaa tcttcatcat tattctttgc taaatcaaaa attatttttc
     5881 catttaagtc attttcattc aaatttttta tttttgctac ttttttaatt aggctgctag
     5941 tagatgccaa ttcttgaaaa gaacttccat ttatattcat atatcctact tcaccagcac
     6001 tattactaaa cccatttatc agtttattgt ttattataat acatccacca attccagtac
     6061 ctattgttaa acatatagaa gatgttgcac ctctacctgc tccaagccac atttcaccaa
     6121 gtccagcaca attaacatca ttttcaactt cacattttat attaaactct ttttctactt
     6181 ctttttttaa ttgcataccc ttatagtttg gaattaattc ttctaatgca aatagtattt
     6241 taccttcaac tgggtcaacc atacctgcag ttgatatgca tataccactt atttcatttt
     6301 cctctctata tttttttact atatgtttta ttttggctaa tattccttct ccaccttttt
     6361 cctttgcttc agtatccata ctggatttat caattattaa tccatcttct ctaataaaac
     6421 catattttat tgaagtacct ccaacatcta tacaaacgta atttttcata acatatctcc
     6481 atacaaatta tttttataaa ttaacaactc tatttaaaat ttatattaca caaatctttt
     6541 acttatcttt tccaattcac agatatattt ccttgcttct gataagtcta tagtatcact
     6601 taatacactt ttttctttgg ttatatgata cttataaata tttttacata agtcctctcc
     6661 taaaatatta agaccttctt gagtataatg aacatcgtca atagttaagc ctttgtcatg
     6721 aaattgcata gcaatcttac ttcctacaca cagtatatca cattcattac aagcagattc
     6781 ttgagctgct gcaattcgtc tagttctaat aaaatattca tcttctccaa gccaataacc
     6841 agttaaactt acaaacattt tatcaatcat acattcttct ttaagcctat acgcaatttt
     6901 ttttagactt tctttatagt acaatgcatc actaccatat tttgcatcac tttcaccttg
     6961 aatccaaata acgtatttat tttctatatt ataatgttta cttaaggtag tacagcctgc
     7021 attaaatttt tctatagtgt catttagata ccagttatta tcatgattcc aattttttat
     7081 tcttgaacca tcttttgcat tgtgaataaa acaaacttta tttccagtga gcttattcca
     7141 ttcatttgaa aatgcaggtg ctatagtacc atcaccttct gaggtctcta actcacatct
     7201 catatttata atttcatcag tactcaaata ttcaaagcaa ttttcatttg gaatgacgct
     7261 ctcttttgga tttcctagtc ctctagcatt actttgtcct attaataaga atacatctat
     7321 attttccata agttaactcc tttacaaaat tttaagcttt taattatttt caattttctc
     7381 ataaaaaact aaggctgaaa atagtaatct aactaaaatt tacgaagtct cactaatcgc
     7441 tgaaagttaa atcactattt tcagccctag tggttatata aattatcatt atctaaccaa
     7501 aaccttaaaa atagcttttt tgatattttc tttttctaaa actcttattc ctgtcatatg
     7561 agcatcttct ggataacaga ctaaaaaatc accttttcta agttccacgc atccattatt
     7621 tttaccatca agaggtaaga aatctccctc ttcttcatat attttttgtt ctaaattttc
     7681 tataaaattt atatctattc gttcacatcc atcaagcatc aaatgaacat ctatatactt
     7741 cttatgcgct tcccagaatc tatcttcttt ttcacaagtt ttatactcta caatattcac
     7801 aaaaatatca tcaccatcta ttgtataagt acctttttca taatctatta atgaattatc
     7861 tttcgcatat tcaaaacatc taagtaaatc ttcatgcaat gttgaatatg tcttactatg
     7921 atttatattt ccaaatatca taagcactat ctccctatat aattataaaa tctattttta
     7981 tacttttata tataaagtat attatacaat cttatagagt aactaaatta tttatctaat
     8041 cactctataa gacaatttat catttaaata ttatttttca taataaacag tagtctcagc
     8101 ttttggtaaa gatacttttg ctttaacttt agcttctatt gcactaacta ttgtacctac
     8161 cactacagaa gtcgtgattg ttattagaga atatgcccaa gaagttactt ctggcatacg
     8221 gtattttaaa taaacaacta aaattgctga tactatgaat ccaacatatg ctcccatttt
     8281 agttgctttt ttacaaaatg caccaagtac aaatattcct gctaaaacac ctagtactag
     8341 ccccataaaa ctattgaacc attcatatgc tgactttatt tctccatttg ctaatactat
     8401 tgcaacagct atagaaagta tacctacacc taatgatata tattgtgcta tcttagtttg
     8461 tttttccata cttatatctt ttgttatgat agtttgtata tctaaagtcc aacttgtagc
     8521 aactgagtta agccctgtcg ataaagttga ttgagaagct gcatatatcg ctgcaagtag
     8581 tattcctgtt atacctactg gtaattcata agctatatat gatgcaaata cttggtcttg
     8641 ttgaactgtt tgagctaaat ctgggttttg agtatagtat ataaataaac aagttccaat
     8701 tagataaaat actgttgcaa gtcccattga taaaacgcca tttccatatg tcattttatt
     8761 aagttgcttt atgtcagttg ttgtagtaaa cctttgaact atatcttgac tcgatacata
     8821 tgatgaaaaa gtattaagac cagctccaac aaatattata aatgcacttg tagacagtat
     8881 atttttatca aacatgactt cattacttgc taagaacttg tgtccagttg taagagtatc
     8941 cattactgtt ccaaaaccac catttatact tgctattaaa tagaacaatg tccctgtaac
     9001 acctactatt aataccatcc cttgtataaa atcggtccac aagacagctt ttaatccacc
     9061 tgtatatgag taaattattg ctattacacc cataactatt atcagtacat ttacaggtat
     9121 tcccgtaaga ccagccagaa ctattgatgg aagatacatt ataatagaca tacgtcctat
     9181 ttgatatatt ataaacatta aagctccaag cactctaaga cctttgcttt cgtatctcat
     9241 ttctaagtac tcataagctg tatctatttc taacctacta tatagaggta agaaaaattt
     9301 tattgttaaa ggtatagcta taaacatacc taattgtgca aaccataaaa tccaagtccc
     9361 accataagaa tttcctgcta atgataaaaa cgatattgga cttaataatg tggcaaatat
     9421 tgatactgat gtaacccacc acggaatagt tccatcacct ttgaagaact cttttccctt
     9481 catttccttt tttgaaaata acaatcctgc aaataaaact gccaataagt aaaccactag
     9541 tattgcaaaa tctatccaag taaatcctac catttgacta atcctcccat actaaaagta
     9601 ttttattaaa aatactttct gtatatttct tttgctcttg atttcatttc atcagttgct
     9661 tctttcatag gttgtctaca gtatccagcc tcaacacctt gttcttcaag tagtaattta
     9721 attgtttgat ataaaccatt tcctaatata tctgttatta aatcgttagt cacatgttgt
     9781 acttcaagtg cttctgatat tttttcattc ttagttaatt caaatatttg tctagctctg
     9841 acaccgttta cattgaatgt agaaccaatt gccccatcta cacctaaaac tgtagctggt
     9901 aacatcattt catcaaatcc tgcaaatatt aatttatttg ggaatgtttt tctcattctc
     9961 tctaataagt agaaatctgc tgctgtaaac ttaacaccta ttattttttc attttcaaat
    10021 aattctccaa attggtctaa actcatatca acaccagtta aaaatggtat agagtatatt
    10081 attaatctat tatctacaga atttattatt gtattataat agtgttttat ttcttcaaaa
    10141 tcaaatttat agtaaaaagg tgtaacagca cttattgcat catatcctaa atcagttgta
    10201 aatttagcaa gctctacagc ttcttttaaa ttaaccgaac caacttgagc tattaatttt
    10261 atttcttctt tcacttcatc tttagctatt tcaaatattc ttttcttttc atcagttgaa
    10321 agcatgaagt tttcccctgt acttcctcct acataaagac catctacttt acaaacatct
    10381 atattatgtc taattatttg tcttaaccct ttttcattta tatttccttc cttatcaaat
    10441 gaaactaata atgctgaata aattcctttc atatctgttg ttctcatatc tttacctcca
    10501 attttaattt tcaagtatag ttaaatataa taatttttta atttataaat taatttttaa
    10561 ttaatttcaa ttgctttaac aaatttttct gttattaatt gtggtcttgt tattgctgaa
    10621 cctacgactg atgaatgaac tcctagttca aaaacctttt ttaaatcttg aggagtattt
    10681 acctttcctt ctgctattac aggtatttct aaatctttaa ctaatctttc cattaataca
    10741 aaatctgggc cttctaatgt tttagtatat ggagtatatc cagatagtgt agttgaaacg
    10801 caatctacac cgtactcttg tgctttgata gcctcatcat aatttgaaat atctgccatt
    10861 actagaacac cgttttcttt tatatacttt ataagttctt ttaagtcttc attatttggt
    10921 ctaactctat tagtagcatc taaagctatc atttcacacc ctgttgttaa aagttcatca
    10981 acttcttttt tagttggcgt tatatatata tctgaatctt cataatttct ttttataata
    11041 cctataacag gaagtccagt aacttttttt atctcaataa tatcttcaac tccttgagct
    11101 ctaattccaa cagctccacc ttccatagca gccttagcca ttctacccat tataaaagga
    11161 ctatgaagag gttcattttc taaagcttgg catgatacta ttaatcttcc ctttacctta
    11221 tctagcattt tttaaattac ctcgtttctg aaatttattt tcttgattta tgtattaata
    11281 ataacatgaa aactttttct ttgcaatatt ttttcatatt tttttctcac ctctatattt
    11341 ttactgattt attctatata ttagcttgtt ttttaggctt aaaagaaaat ttatttcatt
    11401 tttttggtgt ttattttcat tttatataac cactgttata agtccattta aataatttca
    11461 ataaaaaaac ccatgtatac tatacatagg tttcccaata ttattctaaa tccattataa
    11521 tttttcttcc acagcatgga caaaattcag cctctgtatc tatctcattt tcacaataac
    11581 cacaaactat aaaatcatca cattcatttt ccaattcaaa ttttttacca cattcagcac
    11641 aatatttatc atttacattt ccaacataac cacactcaca agttacattg ctttctgcca
    11701 tttccatttc attaatttcc atattctttt cataaattaa cttatcaagt tctagtaact
    11761 catcacagaa tttatcaaaa ctattatcat taatagaatc ttctctaatt ttttgatatg
    11821 tcagtattcc catttccaag aatatctttg ctttcttttc ttgaatatca tcaatctctc
    11881 tatttaattt tgatttttct ttatataatt caactttaaa ttttccacta ccaaactttt
    11941 tcttaacctt agaaaattta tcatctactt tattatttgt atttccttcc acagaagttt
    12001 cctccaatca tatttaatca gctgagttta gcttataacc tattaaagat atcacaaaac
    12061 tatatacaaa tgaaattaca atagcttgta agactttaaa ttgcattatt aaagttgctc
    12121 cataactagt tgtattaaag aaattaatat tactatctag tataaatgtg ctaaataaag
    12181 ccaacattcc catcaaaaat gcataatata tactaaatac tattataggt tttatactat
    12241 ctatattata cttaaatctt agcttgtatc catttaataa taatattaac atagacaatg
    12301 ctcccattgc ataaaacatt aatttagtat ctccaaataa agatgaagaa cttaaagtaa
    12361 acattgatac tgttgaattt attatggtta ctgaaattcc atttgcaaaa gcccacatat
    12421 agctagctat ttgtggcagt atactaaata aatttaatcc atttgaataa ctactcatat
    12481 ctagttcatt taagtaacta ctatcagata tagttagtac caatagtatt actaatacta
    12541 aaacataacc taatataaag gttcttatag aatttgaaaa aagagataaa tacatatttt
    12601 ccttttcata acttttttta taagtagtta tgtatgtaca tataaaccct aatacaaatc
    12661 catttaaaag aacactaaaa aattcataac tatattctaa tgcatatcca tattctaaca
    12721 tactatgtga actagttttt acattagtaa aaatagataa aatagctaaa attaatccat
    12781 aaaaaattcc aactcctaga gaatttgcta atacagtttt tgaatctcta catctttttc
    12841 tcataaaaat taggtttgat attaataata ccaaaatagg gattataagt aatatcaaca
    12901 aacctatatt ggcatttata aacccagaac ccatcattgt agacatagat acactaattt
    12961 gacccaagtt gagagctagg attatatgtg ctggatttat taaataacta atatcattaa
    13021 attgaatagt tataatagct ttaaatatca atgcaataat aaatagtata aatatagttg
    13081 taaatgttgt taaaaataca cctcttttat ttgcatgata tgaaatatct tttattttag
    13141 atttcaagtc tagttttgtt tcaagtggtc ttaatgatgt tatttcataa agttcatttc
    13201 cacaaaattt acagtaatta tccttagtat tactttcttt attgcaacta gaacaaattt
    13261 tttcttcttt acttttaaga gtgatatgac tattttttat ttttctagtt ggttcacctg
    13321 tctttttgga ataattatta tgtgaataat tcatattatt tattttcttt tcacccattt
    13381 tttccctcca agttttacta ttgtaaatta tcaaatataa ctatatcata taaaacttaa
    13441 ttatacctta attggatata tttatctatt ttatttgttt attggtgttg ttttttaaca
    13501 ggtcttgata tttaaatagt ttgttaatgc gaactttatt atttatattt tattatgctt
    13561 aatttaaatt tattctattc tttattttca gtagcaaact atctgtaaat cttaattata
    13621 ataaagtatt aatcaataaa gttactcgac attagctaat aaattttatc tatcacaatt
    13681 ttagattgtt gcacacaata tattttgata taattaaatc ataaacgaag tattccaagt
    13741 atctaaggac agaaagggat acttattttt atgctttatt tttaatggag gcttatatga
    13801 acttagaaaa aataaaaaaa ttttttggat ttcctctaga caaaaaacat aaaaaaaatg
    13861 ttgagaaaga actctgcaat tatcttcatc atacgggtaa aatcatttgc ataataatag
    13921 taatatctca aataattatg atgatatcaa tctcagctag agaaggaggc ccttttgcca
    13981 caccaagacg ccaatggtat ttttatatgt atgtaacact attttgtgta tcttctgtct
    14041 ttatgtttgt attttcttat ctttggaata aaaaaaagga ttgttatacg ttttatataa
    14101 atttatggat aatttatgtt acttttttct gtctatgggg ttgtgtaatt actctatttg
    14161 accagttagg tggaaatgac ctaagtgtat ttagttacat tatgttagca gctgcagctt
    14221 tctcagtatt aaaatttgga gaatcagtaa tagtttttgg aggatgcttt ttgtttctta
    14281 atttattctt acctgggatt caaactatac caggcaatga gtttagtaat attgtcaaca
    14341 cattttttat aacatccctt gccatttttg tttctacaat gctatataga aatcgtgtat
    14401 ttacttacta tgataaactt ttaattagtc aacaatatga agaaatatct catataaata
    14461 atttacttaa cgacaaagtt atgacagatg accttactaa aatgaacaac cgtagatacc
    14521 ttgaagatat tctacaaaaa aaattaatca gccatgcaaa tcaagaacct ataactggaa
    14581 tgatgataga tatagattat ttcaaacaat ttaatgataa ttatggacat caaaagggag
    14641 actattgtct acaaaatatt gccaaagtaa ttttagattt tacaagctct gaaaatgaac
    14701 atgctatacg atatggtgga gaggaatttt ttatatgtat ttttaactgt gatgaggcag
    14761 atgctaaact taaatcagaa tgtttacgaa aaattataga gtcctgtaat tttgagcgtg
    14821 atgatgttcc tttaaaatgt gtaactgtta gtattggagt ttatacaaag aaaattgaaa
    14881 agacaataac attaaatgag tttataagta attgtgataa ggctctatac actgcaaaag
    14941 aacagggacg taattgtgtt gcattttatt aaaaagttac atactattac ataaacctat
    15001 aagtattata tcgcaaaata aaagtaatca aattatatac ttatagtaca ataaaaataa
    15061 cttgcatatc tctaaaatag ataaatatgc aagtcatttt agtgtttata cttaaagcta
    15121 tataagtttt attatatata attattaaaa ttcagcattt ttaggagttc ttgggaaagg
    15181 tataacgtcc cttatgttag acatacctgt catatacata attattcttt caaatccaag
    15241 accaaatcca gagtgagtag cagtaccaaa ctttctaagt tctaaatacc accaataatc
    15301 ttcttctctt aatcccattt cattaattct atctaacaat acatcttctc tttcttctct
    15361 ttgagaacct cctacgattt ctccaactcc aggaactagt aaatccattg ctctaacagt
    15421 tttaccgtct tcattcattc tcatatagaa agcttttata tcttttggat aatctgtaac
    15481 aaacactgga caattatata tttgctccgt tatatatctt tcatgctcag tttgaaggtc
    15541 acatccccac tcaacaggat actcaaattc atgaccagat tcaattaata attccactgc
    15601 tttagtatac gttattctaa taaactcaga gtttactatc ttatttaatc tttctaacaa
    15661 tcctttatct ataaaactat taaaaaattc catttcttca ggagcatttt ctaatacata
    15721 acttataaca tattttatca tatcttctgc aagttccatg ttatcttcaa ggtctgcaaa
    15781 aactatttct ggctctatca tccaaaattc tgaagcatgt cttcctgtat aagagttttc
    15841 agctctaaat gtaggtccaa atgtataaac gtttctaaaa gaaagtgcca ttatctcggc
    15901 atttaattga ccactaactg ttaaattagc ctcttttcca aagaaatctt gagtatagtc
    15961 tattttgcca tcttcagttt ttggaatatc atttaaatcc atagttgtta ttctaaacat
    16021 ttctcctgca ccttcacagt cactacctgt tatgattgga gaatgtgcat atacaaagtt
    16081 tctttcttgg aaaaatttgt gtattgcata ggcagctatt gatcttactc taaatacagc
    16141 tgaaaaagta ttacttcttg gtcttaaatg tgctatcgtt cttaaatatt caagcgtatg
    16201 tctcttcttt tgtagtggat acgaactatc tgattcccct tctacaacta tactttttgc
    16261 atgtatttcg actggttgtt tagctccttc tgtagatact aactctccct ctacaagtat
    16321 agatgagctt attgctaatt ttcctacctc tttaaaattt tctactttct catcaaatac
    16381 tatctgcata tttttaaaga agcttccatc atttagctct ataaatccaa aatttttaga
    16441 tactctagat gttcttatcc atccagctac ttttacagtc ttccctatgt actgttcttt
    16501 ttctctgtac aattctttta ctgttataaa ttccttttgc attttaaatc ctcctagtat
    16561 aattgttatt gatttctttt aaatataatt tcaataaaaa aagcccttca tccctatata
    16621 aagggacgaa agactattac tctcgcggta ccaccctagt tgtcataatg acctcttagt
    16681 taagcaatgc ttaaagccta taacggtgcc ttccgtctaa gtctacttac taaataagct
    16741 ttcgattaga aactccaaga cgttcttcaa tataaacctc gtattgagct ttcaccatcc
    16801 tcaactcgct agaactacat tttatatcta ctcttctctt cacagtattt atcttttatt
    16861 atttctgcta attaacaaaa ttataccaat ttataaattt ttagtcaata aatttatata
    16921 attgatactg attgttattt aaaatataat caatctaact tgcataatga taataaatac
    16981 ttatctttat tacttcttta ttgcttcaat ttccaaaatc ttataattta cttccattgt
    17041 caacccaatc ttttgcttct tcgacaccct taattgaagg tattgaaact ttactatgag
    17101 gttttaattt ttcaacatca gcccatgctg ctgtcgactc atttccaact aaaagtgaac
    17161 ttttaagtct tttattatct ttacctttta atgctcttgc ctttttactt tccaaaacta
    17221 tcacctctat tatttatttt gtaatagttt tagcaatttt acaaataaaa ttcataagtc
    17281 taaattataa tgatttttaa attaacatca tatcttttca atacttaatt tatacctatt
    17341 tatatataaa atatttactt tgtgtattca aaatataaaa ctgttttttt aattttcata
    17401 tttatttttt atgctatttt tccaccctat ctagactaca taataaaatt taactattaa
    17461 tcaattataa ctttacatag gttcttatct atagagtatt tgctatctgt tgaattgtat
    17521 tttttaaatt taaaatccca tatccctgcg atacatttgg atatgtatat atctcaagtt
    17581 ttgtagcacc caatattaaa tatgttttta aaacctgtgt aaacaatgat aatctcggta
    17641 cattatcctg tttttctaag tattccatca acaaggctaa aacacctgtg actatagaac
    17701 tactcactcc tgttcctgtt cctgtattat atgctccatt tttatatgtg gatattatat
    17761 ctacacctga agctactata tctggcttaa ttcctcttcc tctaattggt cctttcgaag
    17821 aaccaatcca catactatca gtcttattat taaatgtacc aactgtaatt acatcatcac
    17881 tagcagcata catagttatt gtagctactg aatcagggtc taaaaatctt gtatctggag
    17941 ctatcaaatt tttatttggc aagtatatgt catattctcc acctattata agttctgaag
    18001 tcaatcttaa aatccatact ccaggtttta tatctcttaa tcttatctcc agattttctt
    18061 ttccagaagt aatatatgga taaataaatc tcattgcata agtagtattt tctaagttaa
    18121 attttcctct atatatataa aagtctggag aataccttat atcatgacta acttcacctg
    18181 aaggagatat tatttgtgct cctattttat ctggaccatt cgtatttagc gttatatcaa
    18241 gtgcatagtc atctccgtct tgtattatta catcttgaac ttctcctaca ctgttaaatc
    18301 tgccagaata atgaatatct gtattccctt gatttcctgc accactcact actaccacac
    18361 cagcactact caatatatta aaagtatcta gtattgaagt agttgctaca gctcctgatt
    18421 taactccaat agtcaaattt attattaatg gtttattttc tgttcttgct atattagtaa
    18481 catacgttat agctgctaaa aaatctgaaa cactgtaatt tatccttcca gcataataag
    18541 tatctgtata tgattttaat ttaactacta ttaagtctga ttctgtagta atacctctgt
    18601 attgagaatt tacccttcca tttccagcta atataccaga aactaaagtt ccagtaccta
    18661 tattatcttg actcaaactt ccatcatttc tatttatggc tatatttaac tcacttcttg
    18721 taaattcact tccaaaaata aaaccttctg gaggtggatt tgtatttgct tcttggtccc
    18781 ataaatataa aactttacta cttccatcat catttataaa atcaggatgc aaataatcta
    18841 tcccagaatc tataactgct aataaaattc ctcttcccgt tatatcatta tatggatttt
    18901 cataaatata atcagtttca gctgctgttg taatggtttc tccattgttt acattattag
    18961 ttatttcaat tagactgctc attggttcag attcttccca ccaagcaact tgtattatat
    19021 tatttaaaat agtttcatca aaatcaatag gtacatatat tactgcaagc tgactattta
    19081 aaaccatata cttatttatt ccattttctt gtagggcaga ttctatatta ccttgataaa
    19141 ttatacaata agatttttcc ataaatccct cctatcttaa aacatcaaac attcctctaa
    19201 cgtccaaaag cccatatcca gaatttgtat ttggatatac tgtgtttgaa tcttttctag
    19261 caccagcttg cataaaagtt tttatttttt gcacatatgc ttggtttggg tatctaccat
    19321 caacaaaagt atattgaaaa tacattgcag cagcaccagc tgcatgtgca cttgctgctg
    19381 ctgtaccagt tatagttgca tacgtgttcc caggataagc agctattata ttaacacctg
    19441 gtgctactat atctggtttt aacctgtctt caatagttgg tcctcttgat gaactttgcc
    19501 ataaactacc attaatagta ttgtaggctc ctatagttat taaatcatct tgaatcgcag
    19561 gataatttat agtgtaaaaa gggtctacct ctctaaatct agttccactt tttaaaagtt
    19621 ctctatttgg aaggtataag ttatatcttc ctgttataat ataaactcct actaatcgaa
    19681 tcttccatac acctcttttt gcattcttta aagtaacatt agtaaattgc tgtccagaaa
    19741 aagttgttgg gtatatatat gttatagaat attcagtccc ttctaaatca aataaacctg
    19801 ttactttatt ataatttgaa actccaacac ttttactttc ttcgccagtt ggagatacta
    19861 ttattacatc agctttatct ggtctattta accaaagttc taatgataat tcttcctctt
    19921 cttcatttag ttctaactca acttcaacag aacctccaac atatggtatt attccagatg
    19981 tatgtgtctg tgtattgccc tcatttcctg ctcctgccgt tatacataac cctctagtaa
    20041 agaatgcttt ttcactatta ctcctattgg ttaaacctgc taggctactg gtacctaaag
    20101 acatatttat aactagtggt cttccaagtt caaaagcttt tttatatgca tactgagatg
    20161 cagcaaacaa cattgcacta ttataaaaac catctatctt gccgagtttt attattatta
    20221 gctcagaatc ttctgctata cctgcatatt cactatttac atttccaagc cctgaacata
    20281 taccactcaa catagttcct tgaccaactt catcctgaga tagactaggg tcattttctg
    20341 cgatagctcg atttatatct tctcgtgtat attctgtccc aatataaaaa ccatcaggtg
    20401 gagttccttc ttttgtttgg tcccataaat atactatttt tgaagtgcca tctggataaa
    20461 taaaatctgg gtgtagataa tctattcctg tatctgcaat tgatattaga gtccctcttc
    20521 ctgttatatt gatattagga ttatttttaa agaaatttac gcccatttct tctgttgcaa
    20581 ctacacctcc aaaagtacct cgattaattt ctccaagtaa tgtcatcttt gtagtcgata
    20641 cagttcttat gattgaagga agttgtaaaa ctcgttgtat gctattatag tctgtattgt
    20701 ttataaatag tacacctaat gaatcactta ttttaaaaaa attttgaccc atacctaacc
    20761 cagctagttc ttcttcaaat tcgggagtat ggactacatc tattcctgat agtattcctg
    20821 attgtctact tgcattttta cttatataaa tttcattttc taagtcatta tgtatttgca
    20881 tattataacc atctataaac ttataactat tttcctcatc tttaaatgac ttattaatac
    20941 ttctatatcc aatatcctct gtttctaaat cttgatttat attagataga gtatacaaat
    21001 ctatatctga taaatttaaa aaaccaaatc ctgatgaatt atttggataa gattgattac
    21061 ttaatctcct tgctcccttt agaagtaatg cccttaattt ctgtgaatac aaaaacaaat
    21121 catttccatt tacaattccc cattccataa acaatgaaca aacccctgta acatgaggtg
    21181 tagccatgct agttccagtt aatgctccac ttgtacctcc tggtaaaaaa gatactatat
    21241 cttcacctgg agctaataaa tcaggtttaa agacacctaa ttgtgtatct ccttcaccag
    21301 aaaatataga tactatatct gttcttgaat taaaactacc tactgttata actctacttg
    21361 ctgttccagg aactgttaca gtaagctctt gagtaggaat cagaaatcta gtatttctat
    21421 ttagtccttc agaagttggt aaatatatat ttacattacc agttactata tctattggtt
    21481 caaatacaat tttccataac ccaggagtta tctgtgtatt tgaacttaat tgtagagtaa
    21541 ctcttcttgt aagagaatat ggtgctatag ggtaaaaata accagttatt ctagtttcac
    21601 ccaaagtatt ccttatctca cctgaagtta gagagattgc ttgtgtttga ttatttgatg
    21661 ggtttaccaa atgtacacta aaatcatcaa caaaatctgg ccatatattt atgtttaata
    21721 tacgctctcc ttccccaact ataaactcaa cttcttctgt aatattattt tgaagcctaa
    21781 tccttttatg acctccttta tctgcattat ttccagctgc tacaactata ttgtttttcc
    21841 aaaataaaca catatcatct atatattgtt caaataaaga tgttcctcta tgtgaacctt
    21901 cattacttcc ataacttata tttaatgtaa caggcattct taattctaat gccctatcta
    21961 aaataaattt aattgccctc ataaactctg tacttctcga aaaaatatcg gtctgtatat
    22021 ttccgactct aaccactatt attctggcat cacttgcaat agttgcacaa atacctgcaa
    22081 catgtgtccc atgtaagcta gttgttgaaa taggtatata catacttcca tctattgcat
    22141 tatttatatc ttcattagta tataatgttc cttctctaaa accttctggt ggatttcctt
    22201 gaatagattg gtcccaataa tataatatct tagacctacc atcactatct ctaaacacag
    22261 gtagagtata atctattccc gaatcaatta taccaattat agtacctttt ccagttaaac
    22321 cagttctatt tttaaatcct gttattcctg tacttgaaaa actttgaaca tcttgagtct
    22381 gtaatataaa aggcttttct ataaattcaa tttctggata agttaaaagt gtatttacat
    22441 cttcttcatt acttgatgtt attattgcat atgatgaatt aagtatctct actgatacac
    22501 ctaattcttc ttctaatctc aatatatctc cattgtattt tacaattaat tcataattta
    22561 ttataatcac ctcgacttct aaaattatta ttaatgtata tgttttgtgt gagtctaaaa
    22621 tgtattagac tatataattt tttaagtttc agttatacaa tttataatag atatattaga
    22681 aaaataaata atgatggtac aattttgaat aaatcatacc atcatttata tttactttat
    22741 cttgaaacca ttaaattttt caatgctttt tcaagacctt ttacagtcag tggaaacata
    22801 ttaaattcac cttgtatcat tctttgagtt tggtaccaat aattaattga atcccatcca
    22861 tctctaagtt ctggattcat ccatacaatt ttactgtact ttctcttaaa ccttttaagc
    22921 cactctatac caggtgtgta attatatgga ccacgatagt taccaccaat atgaagtaac
    22981 tcagaaggcg acatagaagc atctccaaca attataactt tatagttttt atctatattt
    23041 tttaatatcc attcagtatt aatagattta cttaaccaac attcaggtgt tgtaaataat
    23101 ctatcataaa tacagttatg gaaataatat atctttacat ctttaaaatg atttgattta
    23161 cttacagact gaaataatgt attacataaa gtgctatacc ctctcattga accacctgaa
    23221 tccataagca ataaaagctt cactgtattt tttcttggct tctcaaaaaa taactttaaa
    23281 tatcctgcat tattacaagt ctcttcaatc gtcttatcca aatcaagctc tgtctttgga
    23341 gcatctattc tagttgaaaa ttgacgaagt ctctttagtg caacttgaaa ttgacgcata
    23401 tttaaaactt catcatctct aaaatctctg tattttcttt ctcctgcaac ctcaagaaca
    23461 gctctaccat aagtagaatt tccacctata cgtataccag tttgaccttt tcctgaatgt
    23521 cctaactcgg aagtgcctcc tgttcctatc caataattac caccattatg aggagagtct
    23581 tgttctttta ttcgttcctc caatttagct cttaatttat caagatttat atttggattt
    23641 tgttctaatc tttcaaactc ttctaaatct atatctggtt ttttaagcca attcataatc
    23701 tcttctggaa tttcttcttg gtgttctata cctttaaaat actccaaaaa cactgcatca
    23761 aacttatcaa aatcactttc actttttatt aaaatcatcc tacaaagata gtaaaaattt
    23821 gaaaagtttg aatagcacag tcctttatcc aatgcttcaa ttaaagtcat ccattcattt
    23881 aaagatacat ttaatcctct tgacctcaat aaataaaaaa attcaataaa cataattatc
    23941 cagctcctat taactataag tgtctttgca tagattcaat atcctcatta ttcttaagca
    24001 gtattcctgc aaaaggaact tctttttcta ttttttcaat aggcattcca ctcaaagtta
    24061 atgcttgtat ccagtctatt aactctgatg tactaggctt tttttgtatg tcctttaaac
    24121 ttctaatcca ataaaaagta gtcattactt gttccaatag gtgttcttct actttatcaa
    24181 aatgcacttt aactatttct tccatcatat ctctatcagg aaattctata taatgaaata
    24241 tacatcttct taaaaaagca tctggaagtt ctttttccgc atttgaagtt ataataacta
    24301 ttggtctttg tttagctctt acagtttctt ttgtctcatt tatgtaaaac tccattttat
    24361 ccaattccca aagcaagtca tttggaaatt ctaaatctgc cttatctatt tcatctatta
    24421 aaagaattac ttgttggttt gaactaaagg cctctcctag tttgccatat tttatgtact
    24481 tgctaatatt atctacacct tctcctccaa attggctatc ataaagtctt tgaacagtat
    24541 catatacata aagcccttct tgtgcttttg tagttgattt tatattccaa attactaaat
    24601 ctttttttaa ttcatttgaa attgcttgtg ctagcatagt tttacctgtt ccaggttcgc
    24661 ccttaatcaa aagtggcttt tcaagagcaa tagcaacatt aactgatgcc atcaattctg
    24721 gtgatactac ataatcacta cttcctctaa agttagatat ctcttttttt atcataaatt
    24781 ctactccttt taaacagtat tttctaaatg tatatagact ttattactat ttttg
//
LOCUS       DAEMAE010000038        24392 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEMAE010000038 DAEMAE010000000
VERSION     DAEMAE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.039"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            500..1429
                     /gene="glsA"
                     /locus_tag="KPH93_001225"
     CDS             500..1429
                     /gene="glsA"
                     /locus_tag="KPH93_001225"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG1688056.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1527..2090
                     /locus_tag="KPH93_001226"
     CDS             1527..2090
                     /locus_tag="KPH93_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688057.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2578..3414
                     /locus_tag="KPH93_001227"
     CDS             2578..3414
                     /locus_tag="KPH93_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG1688058.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKANNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3698..4693
                     /locus_tag="KPH93_001228"
     CDS             3698..4693
                     /locus_tag="KPH93_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG1688059.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4949..5353)
                     /locus_tag="KPH93_001229"
     CDS             complement(4949..5353)
                     /locus_tag="KPH93_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG1688060.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6239..7153
                     /gene="queG"
                     /locus_tag="KPH93_001230"
     CDS             6239..7153
                     /gene="queG"
                     /locus_tag="KPH93_001230"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG1688061.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVSDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKIFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7259..9496
                     /locus_tag="KPH93_001231"
     CDS             7259..9496
                     /locus_tag="KPH93_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1688062.1"
                     /translation="MKYNNTSEINPLDCILGQERAVEAMELGLKINNPVYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKVQTYLENINKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIASQCNKNDLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAINEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9693..10745
                     /gene="nth"
                     /locus_tag="KPH93_001232"
     CDS             9693..10745
                     /gene="nth"
                     /locus_tag="KPH93_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG1688063.1"
                     /translation="MKNVNLKEINEKDVIVEEEINKKGMDNKDIIVEEEINKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKDENKKKTKKNSSSKATQKPSKETKSKSKIKNKSANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10939..11409
                     /gene="trmL"
                     /locus_tag="KPH93_001233"
     CDS             10939..11409
                     /gene="trmL"
                     /locus_tag="KPH93_001233"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG1688064.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11445..12293
                     /locus_tag="KPH93_001234"
     CDS             11445..12293
                     /locus_tag="KPH93_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG1688065.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMIELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12682..13686
                     /locus_tag="KPH93_001235"
     CDS             12682..13686
                     /locus_tag="KPH93_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1688066.1"
                     /translation="MRFKRILILMLTVVMIVGMFVGCDNNPSNNPSSSKDNKSDVSDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKGSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRSKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            13812..14924
                     /locus_tag="KPH93_001236"
     CDS             13812..14924
                     /locus_tag="KPH93_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1688067.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDIAKS
                     KQSESKSVPTSSEKDKSNNKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAIGARFAEPIKEAATKYS
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIRSVVKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15213..15974
                     /locus_tag="KPH93_001237"
     CDS             15213..15974
                     /locus_tag="KPH93_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688068.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTVEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            15977..16795
                     /locus_tag="KPH93_001238"
     CDS             15977..16795
                     /locus_tag="KPH93_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG1688069.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            16975..17577
                     /locus_tag="KPH93_001239"
     CDS             16975..17577
                     /locus_tag="KPH93_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG1688070.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIVQGLVTMFI"
     misc_binding    17953..18185
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18249..20168
                     /gene="thrS"
                     /locus_tag="KPH93_001240"
     CDS             18249..20168
                     /gene="thrS"
                     /locus_tag="KPH93_001240"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG1688071.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            20934..22049
                     /locus_tag="KPH93_001241"
     CDS             20934..22049
                     /locus_tag="KPH93_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG1688072.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIVEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKELIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIVRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETETRVARETAIRVAKEKDY
                     KIIIMLKKNGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(22271..23308)
                     /locus_tag="KPH93_001242"
     CDS             complement(22271..23308)
                     /locus_tag="KPH93_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG1688073.1"
                     /translation="MNKKLIKAKIFTWSIVAISLSIVFVSLLIGTIISHSFNFPFNIL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVIVKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGDSLSIYDNSAKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTVSGAIGLSGNINKINLDTTSGHIDIAKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKITSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITDLYLSTT
                     NGNLTIIKNNH"
     gene            complement(23301..23987)
                     /locus_tag="KPH93_001243"
     CDS             complement(23301..23987)
                     /locus_tag="KPH93_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688074.1"
                     /translation="MLDKIRTYIDNSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFINAYWIIIVAIYLITSILFNIWFFSWILFIIGAAGINIFKGIIQL
                     KGYKEDDKYE"
     gene            complement(23999..24319)
                     /locus_tag="KPH93_001244"
     CDS             complement(23999..24319)
                     /locus_tag="KPH93_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688075.1"
                     /translation="MTIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITSIGKEKYIKSREEWNNTKALI
                     DILI"
BASE COUNT         9790 a         2408 c         4375 g         7819 t
ORIGIN      
        1 gtaaaagagt aaaagagtaa aagatgtttg gttagaagtt ttgatgttat aaaaatttag
       61 attactagat tactagattg ctaaattact aggttattag attgctgaat taccaggttg
      121 ttaaattact ggattattag attgctaaat tattgaatta ctagattgct aaattacaag
      181 gttgttaagt tactggatta ttagattgat agattacaag gttgttaagt tactggatta
      241 ttagattgat agattactag gttgttaaat tactagatta ttagattgat agattactag
      301 attattatat tgctaaattg ctaggttgtt aaattattga attactaaat tattaagcta
      361 tttaaactat ttaaattgtt tgaaaaatga attaattctt gtagaaaata attttaaagt
      421 ataatttttt ttaacattgt tttattatag tataaacttg tgctaaaata gaatatataa
      481 cataatataa gaggtgcata tgaatacgtt agatgagaca ttattaaaag aaattataag
      541 ttctaataag aagtatacta attatgggca agtcgctagt tacataccag aattaaaaaa
      601 cgctagaaga aatgatttag gaatttgtat catagatagc gaaaataatc tttatagtgc
      661 tggaaactgt agcacaaagt ttactataca aagtatttca aaaccaattg tgcttgctat
      721 ggcacttatg gataatgatt gggaagacgt tttttccaac gttggaatgg agccaagtgg
      781 ggacccattt aattcaataa tgaaacttga aattaatgac acaaaaaaac cctgtaatcc
      841 aatgataaat gcaggggcta tagtaactac ttcccttata aatgggagtt gtttagaaga
      901 aaaagaagaa agaatgctta gtttctttag aaagttagca aaaaatgata atataggaat
      961 aaactatgat gtctataagt ctgaaaaaat gactggagat agaaacagag ctatggctta
     1021 cttattgaaa agtgatggat ttatcagagg gaatgtagaa gatgttctag acctttattt
     1081 taaacaatgc tcgattgaga tagattctgt agatttagcg agaattggaa taaatcttgc
     1141 aaattatgga gtagatatag aaaatggcga gcatttaatg agtgaaatgg tttctaggat
     1201 tgtcaagaca tttatgatga cttgtggaat gtatgatgct tctggggaat ttgctattaa
     1261 agttggcatt cctgcaaaat ctggagttgg gggtggtata atggcttctg tgccgggacg
     1321 catggggatt ggtgtttatg gacctgcgct tgacaaaaaa ggtaacagcg tcgcaggagt
     1381 taaagtttta gaggagttgt caaataaact caaattaaat attttttaaa atattaagaa
     1441 aggatttttt tgacaacaac atataattta atatataatt aaattgtaaa gaaatatgaa
     1501 ttaaataaat tttttaggag gggtcaatga aagaggtatt ggtattacga gatttggagt
     1561 gcataaaggc tattgcgcat ccacgtagaa tagatattct aaaagcattt gacaaatcgc
     1621 cattgtcagc aaaacaatta tctcaattat tggaagaacc tcatgcaaaa ataaactacc
     1681 atataaaaat gttatacaaa gttggtattt tagaattagt tgaggaaaaa ataaagtcgg
     1741 ggattgtgga aaagtactat tatccaagtg caaaaaatgt ggttataggg aataggatac
     1801 ttaatttttc tttagacaat ggggaagaaa aagaagaatt gtacatatcg aagtttgaaa
     1861 atatgagtga agttttttat aaggcagcag aggaagatgt tttagaaaat gaaaatatag
     1921 ttgattatca tgatatttct cttactcatg atgaattagt tgaacttagt gacactatga
     1981 agtcaaagat agatgaaatt ttaaataaaa gacaacacaa tgttgaaggt tctaagtatg
     2041 atatagctat ggttatagtt cctactcttg aggaggaatg tcctagttaa agaattaatt
     2101 atggattaat taaagatata atattagaga ttgaacatca aaatttactt aataatcaaa
     2161 aaatagtttt tggtatgggt aaaacaatga tttttaatct ctattttttt gttttcttat
     2221 acctattgta tttgtaaata atatatagtg tgcaagatgt atacactata taagaagtgc
     2281 ttagataata tacaaagtat gtaagattat aatatttgaa attgattaaa atgggtataa
     2341 ttaaagtgta aatattaaaa ttagaaatta atataaatta aatcaatact agaaatatgt
     2401 taaaagagga atgaggttag ttgggataga gcattcctta gagcttatga atttttagtg
     2461 aaaaagtact atcaaatagg aatctccatc ttcatatgtt ggctgaatgt agttaaggtg
     2521 gagtagttca aaattaaaat tgagtacaag ttgtagtaca aataaaagga gattaatatg
     2581 attaaattag gaacacatct ttcaatagca aaaggatttg ctaatgcggc tgagacagct
     2641 gtatatattg gtgcaaatac atttcaattt tttagtagaa atccaagagg aggaaatgct
     2701 aaagaatttg atgaaaaaga tataattaaa tttcaagaaa ttagaaaagc aaataatttt
     2761 gcaccactgc ttgcacatgc accatatact atgaatttgg gtggcacaaa agatgatgta
     2821 tacgaatttg caacaagggt aatagatgag gatataagaa ggatggactc attaggaatt
     2881 gaatacatgt gctttcatcc tggaagccat gtaggtggtg gtgtagattt tggtatagat
     2941 agaatagtaa aaggattgaa caatgccata aagggcgatg aaaatataac tatacttctt
     3001 gaaaccatgt cgggaaaggg aactgaaata ggttttaaat ttgagcaatt aaaaagtata
     3061 atagataaaa ttgagcacaa agaaaaaata ggtgtgtgtc ttgatacatg tcatattttt
     3121 tcagctggtt atgatatagt gaatgattta gatggggtct tagaggaatt tgataaggtt
     3181 ataggtatag aaagattgaa aactgttcat ctaaatgata gtatgatgcc atttggagat
     3241 aaaaaagaca gacatgctcc tataggggaa ggaaagatag gattaaaagc attaattgat
     3301 tttatggaac atcctagtct aaaacattta ccatttttct tagaaactcc ttttgatgat
     3361 gaaggtcaca agagagagct taagatgata aaagaaatac tatctagtaa ataaaacttt
     3421 attaaaaata tgtgaaatac tccgccctca acatatttag ggtggagata caatcatgaa
     3481 atgtgataaa ttaaattaat ggataggaat aacaaaaatt gtatacaaat taactaggta
     3541 taaagtatga ttatattatg aatagactga tataaaatat aattatatta tgaatagtat
     3601 gtaaatatat tgcaaagatt ctcataattt gttgtataat taaaggtgac atatctatat
     3661 tatataaaaa attattaaaa gttaggtggg gaaagatatg agaaatttag gtaatactaa
     3721 catgaaaata aagagagttg gctttggggg cattccaata caaaggatta ctcaagatga
     3781 tactaactta gttataaatg agttagaaaa acaaggaata aactttattg atagtgcaag
     3841 agggtataca ataagtgagg aagcaatcgg aattgccatt gaaggaaaaa gagataaatt
     3901 tttcttagct actaaaagta tgtccagaga ttatgattca atgaagagag atgttgaaat
     3961 aagtttaaat aattttaaaa ctgattttat agatttatat caattccata atgttaaaga
     4021 agaggaatat gataatcttt ttaaagataa aatggcttat agtgcccttt tagaggcaaa
     4081 ggagcaaggt aagataaaac atattggtat aactagtcac aatttaaata ctatagaaaa
     4141 agccatagaa gatggaaaat ttgacactat acaattccct tataatatag ttgaaggaca
     4201 agcagatgaa gtgtttaaaa aagctcatga aaaaggaatt gggataatag taatgaaacc
     4261 tttagctgga ggtgctttag acaatgcaac actggctatt aaatatatac tatctaaaga
     4321 ttacatagat gtagttatac caggtatgga gagtgttgaa caggttagac aaaatgtcgc
     4381 tgtacttgaa aatcttgtgc tagatgaaaa agataataag gaaattgaag aaataagaaa
     4441 ttctttaggt aaaaaattct gtagaagatg tgaatattgt atgccttgtg ctgtaggtat
     4501 aaatatacca ttaagttttt tatgtgaagg ttattatact agatatggat taaaagagtg
     4561 ggctaaggaa aaatatgagg ttatggatgt taaacctact gagtgtattg actgtgggct
     4621 ttgcgagagt agatgtccat atgaacttcc tataagagaa atgcttaaga ctgttgttga
     4681 aaagctagga tagattttat ttattgaaat cggtgtatta aatttactcc gatttctttt
     4741 tattagatgt ggtcttatat acaaatatta aatttacata gttatagcta ttatataaat
     4801 ttaatatttg tttaatactt actaaatatt tgcttaatgt ttttaaaaat atttgtttga
     4861 gtatagatat aacacaatta ttattgttgt attagtatag tttcaaatac tggagcatca
     4921 atataatgtc gaataacaca taaaatcttt atttttcaaa aatattatag gatatatcgt
     4981 atttagatac tggataaagt tcaatttcaa ccctagggtt gtccttatct ataaaatcaa
     5041 ataaaagtac tggcttaagt tgtttatcat tttttattat tccacttttt tcaataccat
     5101 cacaaatact ttttggataa ttatgtaaat cccccattct cttatttggg aagaataatt
     5161 tcaagactgt tattagattt tcttcaacag tttctccttg atactgttgg tttataaaac
     5221 ctgcaatcat gttttcgtat gcaagatact tagaatattt tccctttgaa ggcatccatg
     5281 cttgaccatt tatgttatga agcttaaaat tagatttact aataggtcta cctggaattg
     5341 ttattttaat caaatttttc accatcctta tacagtattt attataatct gtatatgatt
     5401 taaaagcaaa ttgttattta aagtgtttta cagagtttct ttgtaaaatc ttccacttta
     5461 gatgtagtaa tataagataa gtgttttgct tacaagtttt aaattttgag atatgctgga
     5521 catattcata ttagactatt ataagacctt cataatatta ataagtataa gtttagtaaa
     5581 gagagtaaaa agaaacccat ataagtcaag taaagactta ataaatggca atgtaaatgg
     5641 aatattaaat ataagaaaaa atcaatcttc ttgcattaac tataggaaga atgtcaaaat
     5701 ataactaatg atatttgaag ctttagagtt tatattaaaa ggtaagaagg ttaagtgggt
     5761 aaaaaaggtt gaatagatta aaaaagctaa gtatctaaaa gagttagtag ataaaaaaat
     5821 taaatatcta aaaaagatta agtatctaaa agagttagta ggtaaaaaat taaataggta
     5881 aaaaggctaa gtatctaaaa gagttagtag ataaaaaaat taaatatcta aaaaagacta
     5941 agtatttaaa agagttaagt aggtaagaaa gttaaataaa taaatgaaaa agactaagta
     6001 tctaaaagag ttagtaaata aaaaatttaa ataagtaaat aaaaaagatt aagtatctaa
     6061 aagagttagt agataaaaaa attaaatatc taaaaagatt aagtatttaa aagagttagt
     6121 atataaaaaa gttaaataaa taaaaaaaac taagcattta aaagagttaa gtagctagaa
     6181 aaggttaaaa aaattaaata aatttgaaaa gttaattgga ttaaaaagga ataattttat
     6241 ggataataag gaattaaaag cattttgtaa tttaatagga ttaaactgtg taggagtagc
     6301 aggggtagat aagtatgata atttagaaaa gatacttaag gacagacaac aaaaaggtta
     6361 tttaactggt atggaagaac caataattga gaacagaatt aacccaagac atataatgaa
     6421 ggatgctaac tctataatag tgtgtgcatt tccatatcat ataaatagac aagaaaagaa
     6481 taaaaattca aatttatcaa aatattgtca tggaaaggat tatcatattg tagtaaaaga
     6541 ctttttacaa caaatttgcg attatatttc taaaacagta tcagatttta aatacaagtt
     6601 atttgctgat aatggaccat tagttgacag gtatttagct tatttatcag gaataggata
     6661 ttttggaata aataataata tcataactga tgaatatggt tcttatgtat ttatagggta
     6721 tatagtaaat aattataaat ttgaagaaga taaaccacta gaaaaaagtt gttttaaatg
     6781 tggtaagtgt gtaaaatatt gtccgggaaa tgctatattg ggaaattatg atatgaatcc
     6841 caaaaaatgt ttatcatata taactcaaaa aaaaggagat ttgtcagaga aagaaaaagt
     6901 tgttttaaag aatggcaaaa agatatttgg atgcgatatt tgtcaagaag tatgtccaca
     6961 taatgttgaa ataccaacaa ctcatatttt ggaatttaaa gaaaatataa tagattattt
     7021 ggattatgat gagatacaaa atatttcaaa taaagaattt aaaaggagat atggagatag
     7081 agcttttagc tggagaggga gaaatataat aaagagaaat atggaaataa ttttagataa
     7141 gtatgatgac taataataag agtctgtttc atatacttta atgtgtattt actttaacac
     7201 ttgttgtgta aaagtcatat tatatcatta aagttaaata tggggaggtg ggggaatatt
     7261 gaaatataat aatacatctg aaataaatcc actagattgt atattgggtc aagaaagagc
     7321 tgtagaagct atggaattag ggttgaaaat aaataatcca gtgtacaaca tatatatagc
     7381 tggtgaacct ggaactggta aaagtactta tgcattaaag gttttaaatg aatatgcatc
     7441 caaaaaaaat acgcataaag actggtgcta tatatataat tttgaaaacc ctagagagcc
     7501 tattatagtt gaattagaaa aggggtttgg aagagaactt aagaaagata tggagaaatt
     7561 aattgaaagt cttttagaag attttaaaaa tgcgtttgaa agtgaaaatt ttgaaataga
     7621 aaaaaataaa cttctggatg aatatgaaat agaaaaagat atgcttttga agcaaattaa
     7681 gaaatatgga gaagaaaaag gatttaaatt aaaacagagt aaaatgggaa tagtttttat
     7741 ccctttgaaa gaagaggaag atgaaagtga tgagtctgat gaagagtttt acaaagctaa
     7801 aagagaacta gaaaatatgg cgatacaggt cgtatataaa attagaaact tagaagaaat
     7861 agctgaaaaa gcagtgttag agcttgaaga agagatagct aaacttgtag tagaacctca
     7921 tatcaagagg ttgtgtgaaa aatatgaaaa ttatgataag gttcaaactt atctggaaaa
     7981 tataaataaa gatataatag aatatatgta tctgttctat ttagatgaag aagagttaaa
     8041 agataagtat gataaagagc attttattaa atataaaatc aacctatttg tagataatgg
     8101 aagctcaaaa aataaagagt cagcacctgt tgtagtagag ataaacccaa gtccagcaaa
     8161 tctatttgga aaggcagagt atgattatgc aaacggaaat ataaagactg atttcacaaa
     8221 actactatca ggagcatttc atagggctaa tggtggttat ttagtacttt atgcagacca
     8281 attactaaaa tatacaatgt cttgggaaat tcttaaaaag actttgcaga ctaaaaaagt
     8341 aatattggaa acacaaacag caattaaacc agagaatatg ccattggatg ttaagttagt
     8401 actgattgga agccactata tatatgatat tttatataga tatgatgaag attttgaaaa
     8461 atattttaaa gtatttgtag actttgatag tgaaatggat aaaaatgaag ataatgaaga
     8521 gggaatagcc agatttatag catcccaatg taataagaat gatttaaggc attttactaa
     8581 atcggcagtt gaagaggtta ttaaatttag cactagatta tctggtgatt tagaaaaact
     8641 atcaactaag tttaataaaa taatggaagt tgtaatagaa ggaagtgctt atgcagaatt
     8701 tagaaattca gagtatacag agcagtgtga tgttaaaaaa gcaataaatg aaaagagaaa
     8761 aagaatcaat agagtggaaa cacatatgga tgaatcaatt gaaaatggat ttactctaat
     8821 agaaacagag ggacgaaaag taggagtgat aaatggtctg tcagtcttaa gtacaggaga
     8881 atactctttt ggtagagctt caaggattac agcgaccact agcccaggaa gtaaaggcat
     8941 agtaaatata gaaagagaag taaatatgag tggctctata cataacaaag gagtgcttat
     9001 attaggtggc tatctttctg agaattttgc acaggattta ctattgtcac taaatgcata
     9061 tgtttgcttt gaacaaaatt atggtggaat agatggagat agtgcatctt ctgccgaatt
     9121 atatgtacta ttatcatctc ttagtggagt atcaattaaa caaaatattg ctgttacagg
     9181 ttctataaat caaaagggtg atatccaagt agtaggtggt ataagtgaaa agatagaagg
     9241 attttattct atatgtaaaa ctaaagggtt aaatggtgag caaggcgtca taattcctag
     9301 aaaaaatcaa agaaatctcg ttttatcaga tgaagttaat gatgcagtgc gagatggaaa
     9361 atttaagata tatactgttg aaagagtaga agaggctata gagatattga cagatgttaa
     9421 atttgaagaa ataaagaaac tggtaaaaga aaaattaata tctttcagca agatacaaac
     9481 tgtgtcaaaa gaataagttt attatagaat ttaatgggta tttatgtata ataaaaagca
     9541 agaactttaa taggtttttg ctttttttgt aaatatatat aagttaaatt cttattttgt
     9601 atgttttata tattgatgtt tattttttgt tgatatgact aaactattta taaatagtgt
     9661 tttataaatt ggtattttaa agggggataa ttatgaaaaa tgtaaatcta aaagagataa
     9721 atgaaaaaga tgtaattgtg gaagaagaga taaataaaaa aggaatggat aataaagata
     9781 taattgtaga agaagagata aataaaaaaa gtatagataa gaaagatata attgagaaag
     9841 aagagataga taaaaaaaat gtaaagaagg tcgaggttca aaaaggaata gataaaatga
     9901 ttggtaaaaa agatgaaata attgatgaga aagatataga gaattttatg gatattgaag
     9961 aagataaaaa aagtatcaaa gtaagtaaaa aaagtactaa aaaagatgaa aataagaaaa
    10021 aaacaaagaa gaatagttct agtaaggcta cacaaaaacc aagtaaagaa actaaaagta
    10081 agtctaaaat taaaaataag tcagcaaatg aaaaagacgt aaataagatt ttagacgagt
    10141 tagaaaaact gtacccagat gctaaatgtg agttaaatta tgggacagcc tttgaacttt
    10201 taatagcaac tattttatca gcacaatgta ctgatgtaag ggtaaataaa gttacttcag
    10261 agttgtttaa aaagtacaac acagctagag actttgctaa tttgagcatt gaagaaatta
    10321 gtaaagagat aaagagttgt ggtctttaca aaagcaagtc ccaaaaaata aaggatacat
    10381 ctgaacagct atgtgaatta tatgatggtg aagtacctga tagtttagaa aagcttataa
    10441 aattacctgg tgttggaaga aaaacggcag gggttgtact tagtaacgct tttaatcatc
    10501 cagcaatagc agtagatact catgtattta gagttagcaa tagaatagga atagtggacg
    10561 agccaaatcc tcaaaaaaca gaatttgcac ttatggaagc tataccaaaa gaaagatggt
    10621 cacactcaca tcatgtatta atttttcatg gaagaagaat gtgtaaagct agaaatccag
    10681 aatgtgcaag ttgtccaata aaagaagatt gtaattatta taaagaactt aatgaaacga
    10741 aataactttt agaaagtaag ttgtacaagg gggattttct gatataataa tacttgtgaa
    10801 aatgttagat aaaaaactat tatatacatg aaataaaaat tattattaac attattttat
    10861 ataatagtat aatattattg ttattctgtt tataaaataa aatttgataa ataaaagcga
    10921 tacttaggga ggattaaaat gtcattaaat atagtattag tagaaccaga aatacctcaa
    10981 aatacaggaa atataataag aacctgtgca gcaacaggtt caactttaca tttagtaaaa
    11041 ccactaggtt tttcattgga tgataaacac ctaaaaagat gtgggcttga ctattgggat
    11101 atagctgata tacaatatta cgatagcttt gaagaattac aagaaaagta tccaaatagt
    11161 aaatattttt tttctacaac aaaggctaaa cagtcacatt cagatgtgaa atatgaagaa
    11221 aattgtttta tagtatttgg gaaagaaaca aagggattac cagaaccttt gttgaaagca
    11281 aatatggata cttgtattag agtgcctatg ttggatgttg aaaaagctag gtcattaaat
    11341 ttatcaaatt cagttgctat tgtagtgtac gaggctttaa gacaaatagg atacccaaat
    11401 atgaggtagt ttcaacaagc ctaaatgaga aaggagcata cttaatgaaa aatctaaaga
    11461 taatctgtga cagtttatcg gacgtgccaa aagatttgtt agataaatat gatatagata
    11521 tagtttcatt aacagttata ctggaggaaa gagaatacaa ggatagaatt gatatatctg
    11581 gagatgaatt ttataaaagg cttagagaag aaaatgtata tccaaaaact tctcaagcta
    11641 cttatgccca atttaaggaa atttttgaga aatatacaaa tgaggataga gagatacttt
    11701 atatagcagg gtctgccaca gctacaggaa cgtatcaaag tgctgtaatg gcaaaaaatg
    11761 atgtagatgg taaaatatat acatatgact ctaacttgct ttgtatggga actggtttac
    11821 ttgtaataaa agcaggagaa cttgcaagtg aaggaaagtc tgttgatgaa atattgcctg
    11881 ttttggatga attaaaagag aaggcttttg ttatattttc agtagataca ttggaatatt
    11941 tacaaaaggg aggtagaata tcatctacta aggcagcaat aggaagtata ttaaatataa
    12001 aaccgattct tgaaataaga gatggattag tttcacaaat atctcaagta agaggaaaga
    12061 agaatgtaat ttctaagatg atagaattga taaaatcaaa ttgtggggat gatttatcag
    12121 accaaattgt gtatatagga tatagtgatg attttaagga aaaagaacaa ttatctaagg
    12181 ttatgcaaga tgaattaaga cctaaagaaa ttagattttt ccaaattggt gtatgtatag
    12241 gtgcacatgc gggtcctggt gtcactggta tcatatgctt aaaaaataaa taatatattt
    12301 ttggatgaca ttatttattt ttttattaaa ttaggataga atattaatta ttgagatgtt
    12361 aaattggatt tattttggat gaaattaagt gttgatatat tagatttagt ttgtagggaa
    12421 gtgtgtaaat agataagaat ctattgcaca cttttttata gtaagattta ttaaaaaaat
    12481 ttcaaaatgt acttgtaaat tgtatataaa aaattataaa taggatgtaa aagtaaatag
    12541 gatgtaaaag taaataggat gtaaaagtaa ataggatgta aaagtaaata ggatgtaaaa
    12601 gtaaatagga tgtaaaagta aataggatgt aaaagtaaat aggatataaa aatatcgtaa
    12661 atgtatgagg agtgaaattc aatgcgtttc aaaagaatat tgatattgat gttaactgta
    12721 gtaatgatag ttggaatgtt tgttgggtgt gataataacc caagtaataa tcctagctct
    12781 agcaaagata ataaaagtga tgtaagcgat aaaagtaatt caaaaaaatc tgtatcaatg
    12841 atactggata ttgaaggaac aaataatgaa gctatgaata attcagcttt attagcatta
    12901 aacaatgctc aaaagaaact taatatagat acaaacaaag tggaatcaga tgattcctca
    12961 actttttcta atagtataga tattttatgt aatgacaatt acgatttaat tatagctgta
    13021 ggagctagat ttgcaaaacc attggagatg gtagccaaga aatatccaaa gcaacaattt
    13081 gcaattatag actatgagta tgacaagcaa ccttctaata taacttcaat ttcttatgaa
    13141 gataataaat ctggatacct agctggatta attgctggaa agatgactga aagcgataag
    13201 gttggtttta taggaggaac aaagagttct agtagggata aatttgaatc aggttttaga
    13261 gagggtgtta aattttctaa tagtagtata aaggatattt cagttgagta tgcagatgtt
    13321 tttaaaggta gcaaatcagt agaatctata gctaaaaaga tgatggataa tggagtggat
    13381 attatttttt ctactactga agatgattca aaagctgtaa tagatgcagt aaggtcaaaa
    13441 aataaaaaag ttatagcaac gaataaagac caacatgagt tagctccaga aaatgttata
    13501 agttctattg taaaagattt tgagaatcct acttataatc tgatacaaag ctttgtaaaa
    13561 ggaaattata aaggcggaaa agttatagaa aacacagtta agtctggaag cactgggctt
    13621 gcccaaaata gttctcaaaa tattcctcca gatgtgttag aatatgttaa taagaacaat
    13681 aaataatcta ctataatatc caagaatact tatgacttta gttatgaggt gaattggatg
    13741 ttataataga catatataga aagacatatg cagaggtagg tcagacctat ctaaatcagg
    13801 gagtgaattt tgtgaaaata aaaaggatat tgatggcttg ctttgtagta gtttttacaa
    13861 tttttatatt agctatatgt aattttgatt ttgggtttga taaagtaaat aagaataatt
    13921 ctaatataga tattgctaaa tcgaaacaga gtgagtctaa aagtgtacca acttcatcag
    13981 aaaaggataa atcaaataat aaaagtaaga ataaagaaga tgacacagaa gatgattcat
    14041 atgtaagaaa aactattgga gtattgttag ataccgaaaa cttaaataac gaatcatata
    14101 ataacaatgt actatcagcc ttaaaaaggg ctgacgaaga ttttgatgtt aggataaaac
    14161 ttgaaaagcc aaagacttct aaaagtttta aagagagtat agaaaaacta cataagtatg
    14221 agccagattt gataatagca attggagcta gatttgcaga gccaattaaa gaagctgcta
    14281 ctaaatattc caaacagcaa tttgcaatag ttgactatac ttataaagtt cagccagcta
    14341 atgtaatttc tgtatcattt caagataatg ttactggata tttagcagga cttattgcag
    14401 gtaaaatgac tgatactggg aaagtcggtt ttataggagg agttgaaggt tctagtagag
    14461 ataagtatga atctggattt agaaaggggc ttaaaacttc aaataaagat gctgagttac
    14521 tagttaagta ttcaaatata tttgaaaacc ctgaatcagc tagaactatt gcaaaggaaa
    14581 tgagagattc tgatagagat gttgtatttt cagctacaga agatgatagt aaaagagcta
    14641 ttcaggtagc aagagaaaat ggtgggaaag ttatagctat taataaagac caatacgata
    14701 tggctccaga taatgtaata agttcaatta taaaagatgt agaacaacct gtctatgaac
    14761 tgataaggag tgttgtaaaa gatggtttta agggaagtaa agtaatggaa tttaaaataa
    14821 aagatggaga acttggcttg acttcaaaga gtagtagaaa tataccacca gatgttttgg
    14881 aatacataaa aaaagagtat aacaaggtga aggatacata ttaattattg atgtagaaga
    14941 ttataaaaat atactgatta aatttagatt agcaataaaa agatatattt attaaatttg
    15001 tgctaactgt acaaaatata ttgattaagt ttagattagt tataaaaaga tacatttatt
    15061 aaatttgaac caactgtaca aaactatact aattaagctt agattaacta tataaaatta
    15121 taacaataag tgtgtgggca gaaaaattct gctacacact ttatttatat tcacataaaa
    15181 aatttatcaa aaaaaagaaa aaggagtaaa aaatggaatt tacaagagaa ccatttatac
    15241 tagatgacga tttatgtcta gctttggtgg acaaaagaat tagttatcta atggaaaatg
    15301 aattaaaatt aagaaatata gagataataa aaacagtaga atgtaaagaa ttatatgaaa
    15361 gcataaaata tcacccagat atatgtattt gtaatttagg aaaaggagat ataattgttg
    15421 cgccgaatgt atataatcaa tacaaagagt tattaagtag atataaattt aatataatta
    15481 agggagaagc taatttatta aatagatacc cttatgatat accttacaat attgcgatag
    15541 ttggtgacta tgctatacac aattttaagt atactgacaa agcaatatta gattatattg
    15601 aaaataataa acttaaaaag ataaatatag agcagggcta ttctaaatgc tcaatctgta
    15661 tagtagatag aacgtctata ataacatcag ataaaggtat ttggaaaagt atgaagaata
    15721 cagagataga gtgtttattg atagaaaaag gtcacataaa tttatttgag atgaattatg
    15781 gatttatagg tggatgtaca gggttgattt ctaaggataa gctagctttt tgtggagatg
    15841 ttaaaaaaca tcctgactat gaacgtataa aaagttttgt agaaagtaaa aacaaggaga
    15901 tagttacact tagctgtgaa aatttactag atataggttc tatagttcct ctcatgacaa
    15961 gaaaggagcg ataactttgg ttgagaagga taatatttct tccatgaaat ttttgaggaa
    16021 taataatgta aacgatagat taatatatac gaatgacatt gtagaagtag tgatagattc
    16081 tgcaaagcaa gaagtattaa ataaaaaaat acttaatggt actaaagctg aacaaagagc
    16141 tttaaatatt gatgaaatat caacggaaat aactagtatg ataataagta taatgaaaga
    16201 gaaacttttg aaagaataca ttctaaaatg ctatgggcgt acatatcctg aagataaaaa
    16261 taatatatac atatattcac tgaacatatt tgctaaaaaa gagattttta tgagagaaac
    16321 agtatttact agggtgagaa attatataga agaaaatgat tttataaatg tagaaggttt
    16381 tattagattt agaatgagag aatttatgaa atatatttct gctataggtg atattgcagt
    16441 tgaggagtat ttaataaaga aggaccaaga tgaatttata agagttttaa aatactttat
    16501 agatactcaa gaagaaaaga ttgatttgct taaagttcat ataatggaag ataatacatt
    16561 tgttttatat gataaaaatg gaaataagat agacagtata gatgatgaag aaattataaa
    16621 tatggtaata agagaaaatt taaattatga ggacttttta ataagtacct tattaactct
    16681 atgtcccaaa aaaatagaaa tattggattt gttaaataat aattgctcaa aggaaattat
    16741 agatactgta gaagcaattt ttgaaaataa agttagtata attatggaaa actagaatct
    16801 aagtatataa aagttagcca tataaaacta aaaaaattct ttttaaatcg acacaaaaga
    16861 cttataattg tatataaagc tatagtataa ctagagttat taagtataaa gaatatctac
    16921 ttgattaatg caatatagct ggtaaatata caattgataa ggagtaattt tataatggat
    16981 aatttaatta ggatactgat attagctgtt attggtggat ttataggtta tgttacgaat
    17041 gtagttgcta taagactgat atttagacca atagagccta ttaaaatacc tattttaaat
    17101 atagaaatag taggtcttat accaaagaga agagcagaaa tagcagcaaa tgtaggagaa
    17161 ataattcaag aagaatttct ttctatggac gaaatacttg caaatatcat tactgacgaa
    17221 gataaggaag aagttgtaag atacataaaa gcaagggtaa aaattatcat acatgagaaa
    17281 gtttcattta tcccgagtgg tataaaaaac atgatacaag attatctagg agagataatt
    17341 gagtctgaag ttaagcaaag tatagatgaa ttgagtaaaa atataataaa taaagcaaat
    17401 gaacgtatag atatacaaaa aatggtcgaa gataagatta atgaactaga tttatatgaa
    17461 ttggaagaga taataataag aatagctaag aaagaattaa aacatataga gtttttgggg
    17521 cttgtcttag ggtttttaat aggtatagtt caagggttag ttacgatgtt tatttaaata
    17581 tatgagaagt agcatttatt taagtattaa ttgtatttac ttatttgact gtattactta
    17641 ttaacagcat ttagatatta attgtattta agtatacaaa acctaaaaag agtacaaaaa
    17701 ttagaagtta gttttaataa aaattagttt tagtatttat ctagtcatat ctatcatgca
    17761 tgtggatacg ttttaaaaat tttaaattta tgaaaatatt ataatatcac tataaatcaa
    17821 gagattaatt taatataaaa atataagaat gttggtttac agctgattga gatgaaaaat
    17881 aatgggtaat taatatatta cattaatgtt gattgatgta taactatctg ttataatgaa
    17941 aatatattaa aaatcatagt gttgaagagg aagagtagag tagaaaccat tctcagagaa
    18001 tttgggtcta tggctgagag cccatgtaat aggtctattt gaaaactacc ttggagctga
    18061 gcttagtagc agtatttata ctatacgtga aagtgctcgg gtgacgacct tatagtcatc
    18121 aagagggttg tatatacttg tatacgccaa cttgggtgga accgcgaatt aatatcgtcc
    18181 caaatatatt tggggcgatt ttttattaca aaaaatctaa aaattatttc aataaggagg
    18241 aaaaaacaat gattaaagtt gctttaaaag atggctcaat aaaagaattt gaaaatgcta
    18301 tttctgttat ggatgtagct aaatccatta gtgaaggatt agctagaaat gtggtagcag
    18361 cgtctgtgaa cggagaggtt gtaggtctgg accacattat agatactgac tgtgacttga
    18421 atttatttaa gtttgaagat aaagaaggta aggaagtttt tagacataca tcagcacata
    18481 tattagctca agctataaaa agactttacc cagaagcaaa acttgctata ggaccaagta
    18541 tagaaaatgg attctactat gatatagatt tagaccatag attagttcca gaggacctag
    18601 agaaaataga agctgaaatg aaaaaaatag ctaaagaaga tttaaaaata gaaagatttg
    18661 aacttcctag aaatgaagct ttagagctta tgaaagaaca aggagaagac tataaggtag
    18721 aacttatatc tgatttacct gaaagtgaaa taatatcttt ctacaagcaa ggtgatttta
    18781 ctgatttatg tagaggacca catttaccat ctactaaaaa ggtaaaagct gttaaattac
    18841 aaagtgtagc aggtgcatat tggcgtggtg acgaaaataa taagatgctt caaagaatat
    18901 atggaacttc ttttgagaaa aacaaagact tagaagagta cttacattta ctagaagaag
    18961 ctaaaaagag agaccacaga aaattaggta aagaattagg attatttatg atacctgaag
    19021 aaggtccagg attccctatg tttttaccaa aaggtatgga acttaaaaat gaattattaa
    19081 aattctggag agaaatacac agaaaagctg gatatattga gatagaatct ccaatcatat
    19141 taaatagaaa attatgggag acttcaggac actggtatca ttacaaagaa aatatgtata
    19201 cagttaaaat agatgatgag gattatgcta taaaacctat gaactgtcct ggtggattaa
    19261 tttactataa ctctcaacta cattcatata gagatttccc aatgagggtt gcagagcttg
    19321 gtagagttca tagacatgag ttatctggtg cattacaagg attaatgaga gttagagcat
    19381 ttactcaaga cgattcacat atattcatgt taccagagca aattaaggac gaaataaaag
    19441 gagtagctaa cttaatagat ggtatctaca aaacttttgg atttgaatat aatttagaat
    19501 tatctacaag accagagaac tctatgggaa gcgacgaaga gtgggaagca gcagaaaatg
    19561 gattaagaga agctttagaa gagttaggtc taccatatac tattaatgag ggtgatggag
    19621 cattctatgg tcctaagata gacttccatc taaaagattg tttaggaaga acttggcaat
    19681 gtggtactat ccaattagat atgcaacttc caagacaatt tgataatact tatattggtc
    19741 aagatggtga aaagcataga ccagttatga ttcatagagt tgcttttggt agtatagaaa
    19801 gatttatagg tatattaata gagcattatg ctggtaaatt cccagtttgg ttatctccaa
    19861 ctcaagttaa gatacttcct atatctgata agtttatgga ttatgcaaat gaagttaaga
    19921 aagagttatt tgataagggt ataagagttg aattagatga tagagctgaa aaaattggat
    19981 ttaagataag agaagctcag cttgaaaaag tgccatacat gttaatagta ggtgaaaaag
    20041 aagtagctga taataatgtt tctgttagat caagagataa aggtgaaata ggtagtatta
    20101 aattagatga atttatagct agtatttcaa aagaaattga atcaagagaa agtattattc
    20161 aagactaata tacaatatat tagtagctaa gatattgtta tataagataa gtttaatcaa
    20221 tatgttgata tgttgatgca tagtgattaa atttatgaaa agatgagtat aattagaggt
    20281 acattaatca tatttttatg attaatgtac ctctagtttt ttgtatggta gatttattaa
    20341 taaatatatc atagaagtgt tttgaacaag gaaatattta tgtgcattct ttaaataaaa
    20401 gtttattttt tgagtgataa ttaagtactt gttggtaagg catggctgta aaaatcataa
    20461 ctttattttc atatttgttc cattttcata aaaattatta tccctttaag aaaatttctt
    20521 aatccccttg aaatatttcc tttttagtct aaagttaaat ttctttcttc aaaatgaata
    20581 tttaaatgaa ctaaaattta agtacatttt ctaatcttaa tgagtctatt tttcatagat
    20641 aattgcccta taatagaagg agccatgttg gggctatgtg gtaacaataa cggcattgcc
    20701 agtgattaat taatgttttg taaatattaa ttaatgtctt atgttatttt tggacttacg
    20761 ccctatacat agattaattt atttaacttt agatatagag agaatgtcaa atacatacac
    20821 agacgctaga gtttaagaaa gtgtttatgg agcaagaact tttaaaagag gtagtaactt
    20881 agcttaattt caagaaatga taaatacaaa agagtaaaaa gagaggtgtt gaaatgatag
    20941 aaaaagtaga agaaaaatct aagattatta aaacatcaaa agaatataag cgaactatag
    21001 ttgaagaaaa agataaagaa tggaccaaaa aattaaatga catacttagt agaagtagtt
    21061 tttctaatga tgaattagat aaaatcaagg agttaattcc aaaagagatt ttgactagtg
    21121 aaaatgttgg agaagttgtg actgaagatg gatatgctaa gcccgagtat gatgaggtat
    21181 ttaaatcatt gttcacttta aataatgaat atgatttact tgtaaatttt ataaatgata
    21241 ttctaaaaga tgctccatat gctaacagga atatcaaacc gttcactcaa ataaagagaa
    21301 tagttagagt tgaaactgac ccaacaatta attacattgg agagaaaaga cctagactcg
    21361 atattttggc tgaagatgaa gaaaataatc atataaatat tgagatgcaa agagcttttg
    21421 agaacgatta tttggaaaga gctgaatatt atcttagccg tgtacatggt agaaagttag
    21481 aagaaggtaa agagtataaa gaaattggaa aaactattgg aatacatata ctaaatcatg
    21541 taaaatataa tcacatagaa gattatgtaa attgcctgag attaacaatg gacgggcatc
    21601 cagatattta ttctagtaaa actgcattat attttataga gcttccaaaa attcataaat
    21661 ctgactatat agtaaataga attatgctat ggggaaaact tattagtaat ccttctagct
    21721 tagatgtaag agttatagct aagaatgatt ctattattaa aaaagcactt gatagattaa
    21781 aagaattagg tagcaatgaa gaatatttat taaatctgaa aagaggtgct tatatcatga
    21841 ataaaagtag aaattttaaa gaagagattg aaagagaaac ggaaacaaga gttgcaagag
    21901 agacagcaat tagagttgca aaagaaaaag attataaaat aattataatg ttgaaaaaaa
    21961 acggttttaa attagatgat atattaaata aatttaatga ttttgactta agtgaagatg
    22021 agataaaaga agtctatgaa aataattgat gtgtaaataa aaagtgaact tatataagtt
    22081 tgttgaatct gagtctggaa atatttatgt gtatttttta aatgaatgaa aatttatttt
    22141 aaagaccata atttagaggt acagttaatt atatttttat gatactgtac ttctagtttt
    22201 ttctattata gatttattat ttattaataa atctacaata gaagtgtttt tgttgtaaga
    22261 atcattttat ctaatgattg ttttttataa tagttaaatt tccatttgta gtgcttaaat
    22321 ataaatctgt aataccattt ttataagtgt ataaatcact atggcgattc aactcaaagt
    22381 cactgtccaa aacaccagaa actttatcaa aatttaaagt gaaacctgga ttatcaggaa
    22441 tagacaaatc gacattggca gaagtagttt cacagtacag agaagacaac attttgctag
    22501 aagtgatttt tatattactg ctagttgact cagctttaat atcagaaaaa actccagata
    22561 agttcatttt tccagaaact gaactacaag taagattttt attgtatata tcttttaaat
    22621 ttatattacc attagttgtt tctaagttta aaacatcaat tttcccagaa agctgtattt
    22681 caccagatgt tgatttaaga gtagcatttt tactatttag ttttgctata tcaatgtgac
    22741 cactagttgt gtctaaattt attttattaa tattaccaga gagacctatt gcacctgaaa
    22801 ctgtgttagc ttcaactgta ttacttctta tgtaagtgct atatatatct gctgaagtag
    22861 tatataaggt tattttatca taaagcttat tgggcaaata tagttcaagt attgttggtt
    22921 ttggaagatt aattccaatg aagaattttt tagctgaatt atcatatata gaaagagaat
    22981 ctccatcaac cattattttt gctagttttt tctttgagaa attactaggg attttttgaa
    23041 ttaatttgat tttattagaa tcacttctct taactataac ttcacctgaa atccaatcaa
    23101 cattaatgtc atttatttta tctgaaaaac tttcattttt agccactata tagttatcat
    23161 aattgtattt aggattaaga atattaaaag ggaaattaaa agaatgtgaa attatagttc
    23221 ctataaggag tgagacaaat acaatagaaa gtgatattgc cacgatactc caggtgaaaa
    23281 tctttgcctt tataagcttt ttattcatat ttatcatcct ccttgtagcc ttttagttgt
    23341 ataattcctt tgaaaatgtt gataccagcg gcacctatta taaatagtat ccacgaaaag
    23401 aaccaaatat taaatagtat gcttgtgatt agatatatag caactattat aatccaatat
    23461 gcatttataa atgatttata aactggattt atatgattat aattttcctc aaaatcctca
    23521 aattctttga atttatttga taaattgtca ttgtatagag catagtttgt ccttgtcatt
    23581 tgattataga ctaataaacc tactccaagt gctattaata tgagcattaa aagaatagct
    23641 atagttggtt ctatgccaaa acttacaact gagactacaa ataggaaagg gcttattata
    23701 aaaagagcaa ctgctattga tgctcttaag gctcttttct ttttttcttc aattaattca
    23761 gaaggtagag aatatggttc cttaacactt tcaattaact cagacaaatc accaattcct
    23821 gagattactg agttatatgc ttcttgttct gttttaccat cttgtatttg gtcgttatac
    23881 ttttctttta gattttcaaa taattcatct ttaagttctt tgactttttt tgtctcatta
    23941 actcctgtaa agctattatc aatataagta cgtattttgt ctaacatatt ttcacctcct
    24001 aaattaatat atctatcaat gcttttgtat tattccattc ttcacggctt tttatatatt
    24061 tctctttacc tatgcttgtt attgaataat actttcttct agctccagta acttcatcgc
    24121 cccaataaga ggttattgca ccagattgtt caagcctacg aaatgctgta tacaaagtag
    24181 cttctttcaa ctcatacaat cctttagtct tttcaagtat agatttatta atctcataac
    24241 cataactgtc tttttcaagt aaatgtttta agataattgt ttctgtatga cctcttatca
    24301 tgtcagatgc gattgtcatg aacatcacct ccgattaaat atataatact ttgatatact
    24361 tcgcctgtca aggtatatca tgagaaaaga ca
//
LOCUS       DAEMAE010000039        23985 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEMAE010000039 DAEMAE010000000
VERSION     DAEMAE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.040"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            18..335
                     /gene="flgB"
                     /locus_tag="KPH93_001245"
     CDS             18..335
                     /gene="flgB"
                     /locus_tag="KPH93_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG1688076.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDTMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            343..747
                     /gene="flgC"
                     /locus_tag="KPH93_001246"
     CDS             343..747
                     /gene="flgC"
                     /locus_tag="KPH93_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG1688077.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            757..1074
                     /gene="fliE"
                     /locus_tag="KPH93_001247"
     CDS             757..1074
                     /gene="fliE"
                     /locus_tag="KPH93_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG1688078.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQITADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1099..2649
                     /gene="fliF"
                     /locus_tag="KPH93_001248"
     CDS             1099..2649
                     /gene="fliF"
                     /locus_tag="KPH93_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG1688079.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEQEDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2662..3732
                     /gene="fliG"
                     /locus_tag="KPH93_001249"
     CDS             2662..3732
                     /gene="fliG"
                     /locus_tag="KPH93_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG1688080.1"
                     /translation="MSTEITNVNAFDLSDISESDKNEKIEGEGLKKAAVLLMTLGPDV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMTSVSPEVIKVMESSLEDKLHSISGDDFRDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFIFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDAISDAIYRNQSSRAADALREEMEMVGVIKVTQVEAAQQKIVNS
                     VRKLEREGAITIVRNFGDDFVE"
     gene            3716..4456
                     /locus_tag="KPH93_001250"
     CDS             3716..4456
                     /locus_tag="KPH93_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG1688081.1"
                     /translation="MTLLNNKIIKSKKASYKGVLDLSIIDKVRVGESNLNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGKKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFKLFKEETSLKSSVFVIADDSIDKGN
                     AVIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            4460..5779
                     /gene="fliI"
                     /locus_tag="KPH93_001251"
     CDS             4460..5779
                     /gene="fliI"
                     /locus_tag="KPH93_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG1688082.1"
                     /translation="MYKTDIYMDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEQIINFLKQGINEKSSFNETTSSLKRIFE"
     gene            5811..6257
                     /gene="fliJ"
                     /locus_tag="KPH93_001252"
     CDS             5811..6257
                     /gene="fliJ"
                     /locus_tag="KPH93_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG1688083.1"
                     /translation="MELDKNFKFRLQKVLDLKMKDEEKIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQEQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLID
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6286..7491
                     /locus_tag="KPH93_001253"
     CDS             6286..7491
                     /locus_tag="KPH93_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG1688084.1"
                     /translation="MNIQTDSYNVIKDIGDKKSSSKSKNVKNNKFEDNLYKATDNKQV
                     KGRKEMSNQNINSNKEENSENECHLDEDKSIHLEDDSTYEKDFSKNNEILQQLLNLLK
                     NNDKKDKDINSILNDLEKLNISDELKQELKLNKNVLNKIVNQNAKLNNANINNSDDMQ
                     SLDMMKLRELLDNDANKNFEVISKTTENDTKSIDLYNFDSNRMDNINSNKQKDDSSDI
                     LEKLAGGNGNKSGNFGQVINKNLDTNKVKNDNVNLKHIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGENMRAVVTVFNKDVFDSINKNMTDLKQHLELTNVN
                     IKDVSVQMHSDNRNTSDTFDKAFEQQNRQNNQENMNKQNTNNKRNNMAIEDEIKENII
                     DDDRVDLLA"
     gene            7509..8129
                     /locus_tag="KPH93_001254"
     CDS             7509..8129
                     /locus_tag="KPH93_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG1688085.1"
                     /translation="MYGVATALAGAQGSYRSNNLSQLERNTNDIANKQVRSKGNQTLT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDILLALSNSTLINSALSMATSLIGKKVEVSGEEKEDENIVGAVKSTYIKDGEVHLE
                     LKVEGSDKTVDVKYEKLLKIGEVDTNSDEEENSPESGEEESSTEQV"
     gene            8156..9142
                     /locus_tag="KPH93_001255"
     CDS             8156..9142
                     /locus_tag="KPH93_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG1688086.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAAAGPLKDVKGGRLVNGDGYMLQGMIYEGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTIEFLLSDGQRTQFVYN
                     NIPDDAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGA
                     TGTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9155..9358
                     /locus_tag="KPH93_001256"
     CDS             9155..9358
                     /locus_tag="KPH93_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG1688087.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9358..10176
                     /locus_tag="KPH93_001257"
     CDS             9358..10176
                     /locus_tag="KPH93_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG1688088.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10166..10861
                     /locus_tag="KPH93_001258"
     CDS             10166..10861
                     /locus_tag="KPH93_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG1688089.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10873..11316
                     /locus_tag="KPH93_001259"
     CDS             10873..11316
                     /locus_tag="KPH93_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG1688090.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTAENLNNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11331..11705
                     /locus_tag="KPH93_001260"
     CDS             11331..11705
                     /locus_tag="KPH93_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG1688091.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDV
                     VNISKNNSIIILKIGEEGCIMASSSSSINKIKDLSKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11706..12371
                     /gene="fliP"
                     /locus_tag="KPH93_001261"
     CDS             11706..12371
                     /gene="fliP"
                     /locus_tag="KPH93_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG1688092.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12384..12653
                     /gene="fliQ"
                     /locus_tag="KPH93_001262"
     CDS             12384..12653
                     /gene="fliQ"
                     /locus_tag="KPH93_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG1688093.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMVSLITK"
     gene            12665..14479
                     /locus_tag="KPH93_001263"
     CDS             12665..14479
                     /locus_tag="KPH93_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG1688094.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSLTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLIDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVNGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14488..16563
                     /gene="flhA"
                     /locus_tag="KPH93_001264"
     CDS             14488..16563
                     /gene="flhA"
                     /locus_tag="KPH93_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG1688095.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16538..17554
                     /gene="flhF"
                     /locus_tag="KPH93_001265"
     CDS             16538..17554
                     /gene="flhF"
                     /locus_tag="KPH93_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG1688096.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFL
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDIDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIVNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17551..18429
                     /locus_tag="KPH93_001266"
     CDS             17551..18429
                     /locus_tag="KPH93_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG1688097.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENTPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIVDDKKVGLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18443..19144
                     /locus_tag="KPH93_001267"
     CDS             18443..19144
                     /locus_tag="KPH93_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG1688098.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDVEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19160..19582
                     /locus_tag="KPH93_001268"
     CDS             19160..19582
                     /locus_tag="KPH93_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG1688099.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKESIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19567..19752
                     /locus_tag="KPH93_001269"
     CDS             19567..19752
                     /locus_tag="KPH93_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG1688100.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19793..20566
                     /gene="flgG"
                     /locus_tag="KPH93_001270"
     CDS             19793..20566
                     /gene="flgG"
                     /locus_tag="KPH93_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG1688101.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQVIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20587..21348
                     /gene="flgG"
                     /locus_tag="KPH93_001271"
     CDS             20587..21348
                     /gene="flgG"
                     /locus_tag="KPH93_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG1688102.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEALNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFEAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21371..22252
                     /locus_tag="KPH93_001272"
     CDS             21371..22252
                     /locus_tag="KPH93_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG1688103.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVRDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIDLSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22255..22629
                     /locus_tag="KPH93_001273"
     CDS             22255..22629
                     /locus_tag="KPH93_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG1688104.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22661..>23985
                     /locus_tag="KPH93_001274"
     CDS             22661..>23985
                     /locus_tag="KPH93_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688105.1"
                     /translation="MVLILNLNDIKKDILKFNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNILDMYNSANIQTKSNIKVDDYKNEFLHKSSNNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIVIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEGSIDSSLDKKENNIKENEDMNKTSNINKSNKNINKEDVKSKKNTVENE
                     DDKESDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILSE
                     KLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKSSKLT"
BASE COUNT         9886 a         2577 c         4546 g         6976 t
ORIGIN      
        1 tttgaggagg ggtgagtgtg aatacatatg acattataaa aatgggatta gatgcaacta
       61 atctaagaag tcagacaata gcaaataata tagcaaatat aaatactgaa ggatataaaa
      121 gacattatat aacttttgaa gaaacactaa atgatactat gcctaaaata gaagaaaaaa
      181 cagacaattc taaaggttta agagtagatg gtaataatgt agacatagaa gaagaaaaag
      241 taaatcaagc aatggcaaca ttacaatata atgctttgat aagttttgca aatggaaaaa
      301 tagcaatgac caaatcaata ataagtggga ggtaattaga agatgagtgt gttcagtgga
      361 atgagaatat ctgctagtgg attaacagca gaaagtttga aaatggatat catatcatcc
      421 aatgtagcta atataaatgc tacaagaggt ccaaatggag aagcatatag gagaaaagtt
      481 gtttcatttg aagaaaacta tgataataaa ttaggtatgc taggagtaaa agttacatct
      541 gttgaagatg ataaatcacc tcttaaagaa gtatatgatc catctcatcc agatgcaaag
      601 ccaaatggat atgtagaata tccaaatgta aatatattgg tagaaatgac agatttaata
      661 acagcttcac gagcatatga ttctaacgtc gatacattga atgctcagaa gtctatgata
      721 tcaaaagctt tggaaatagg aaagtaggga taaattatgc caattacaca aattaacaat
      781 ttaatgcaag gtgcaaagtt agataatttg attggagaag caaagccatt aactcttaat
      841 aataaagaag aaaaaaagaa ttttgaagat gtaataatag atactataga taaagttaat
      901 tttcaacaaa taacagcaga tgatatgaaa gaaaaattta taaaaggcga agatgtttct
      961 atgcatgaag ttatgttaat aggtcaagaa gcacaactat ctatgcaata tttaatagaa
     1021 gttagaaata agattcatga tgcatatcaa gaaatgaata aggtgcaaat ttagcctttt
     1081 gaacaggagt aataatcaat gataaccagt tttaaaaata agattaagga gcaagttgct
     1141 aaatctagga caaagataag ttctttaaaa aagggaacta aaatagcatt aggtataggg
     1201 gttattgcta tatgtttagc gctagtattt acagcaatat ctgcgagcaa aaataagtat
     1261 gcagtcttat tttcagaact agatgctcaa gatgctgcag atatatcagc agagctagag
     1321 tctaaaaaga tagatactaa agtaaaagga agtacgattt atgtacctag aacagaagtt
     1381 gataagttaa ggctggagtt agcaccaagt cttacaggtg gttctaaagg atttgcacta
     1441 atggatgata gttcatcaat aggacttact gatgaagaat ttcagattaa gaagcaaaga
     1501 atgatacaag gtgaaataga aaaaacaata aagagttttc cacaagtgga agacactaga
     1561 gtacatataa catttggaga gtcatcagta tttgagaagg aggctgttcc agggagtgca
     1621 gcagtttatt tgatgctaaa accagggacg actttagaaa aaaaccaaat aaagtctgtt
     1681 atggcactta tatcaggaag cgttacaaat ataccagaga aaaatgttca ggttattgat
     1741 aacaaaatga atttattatc tgaaggtatt ttggaggatg aaaatgttga tgaaaataat
     1801 caaccaataa atagtggcat tagtgttgat aaaaataagg catctgaaca agcattgaat
     1861 aaacaactag aaaagtctat tttagaaatg ctagaaccga tatttggtaa gggaaaggtg
     1921 aaagctacag taaatgctga tttaagtttt gatacagctg aaaaaactga gataaaaatt
     1981 gaccctgata aagttgcaat aaaggagaca aggtcgaaaa attcatctaa atctgctaca
     2041 aataatgttc aaggtgtaga tgctaacatg aataaccaag gaaataacaa tggtactaat
     2101 acagaagata gtttagatga gagttttgag tatgaaacag gtaagattga aactcacact
     2161 atagttgcgc ctggagaact taatagattg acagcttctg ttgcaataga tggaaaaata
     2221 gctaaaggtg ttcaagctga tgtagaggat attgttaata atgctatagg tatggatgga
     2281 gcgaggggag atactcttag cgttgtttca atggtatttg acccagaagg gaagaaagag
     2341 gctaaacaag agctggaaga gattaaaaaa gaaggattta aaaagaatat aataaaagca
     2401 gttattggag tagtagcagt aatagcagtt ggagctattg gatacttaat atataagaaa
     2461 agaagtgaac aagaagatat ggattatgaa gatgaagatt atatttctaa atctgattct
     2521 gttgatagga aagataatcc tctattagga gatgacccag aactgacatt agaggaagct
     2581 gttaaaatgt atgcagaaga aaagccagac caagtaacag agataataaa aacttggtta
     2641 aatgagtaag ggggatagaa agtgtccacg gaaattacaa atgttaatgc gtttgactta
     2701 agtgatataa gtgaaagtga taagaacgag aaaatagaag gtgaaggttt aaaaaaagct
     2761 gctgtattat taatgacatt aggcccagat gtctctagcg atataataaa aagtttgcct
     2821 gataaacaag ttcaaaaaat aggtttggaa attgcaaata tttcttcaat agcttctaaa
     2881 gaaagaagag aaatcttaaa agagtttgta gaaataaata gagcaaaaga ttatgttata
     2941 gaaggcgggc ttgaatatgc aagagagtta ttaagtaatg ctttgggaaa tgctaaagca
     3001 aacaaattac ttgaaggtat atcaataaat acaagtacaa agccttttaa acatattatc
     3061 aaactagatg taaagaagat attagaaata cttaggaatg aaagtgcaca aactatagct
     3121 gttttacttg cacatatgga accagaaaat ggggcagaag ttttatcata tttagacttt
     3181 gatttacaaa atgaagtagc tatgagaata agttcaatga cttcagtatc tccagaagtg
     3241 ataaaggtaa tggaaagttc attagaagat aaattacatt caataagtgg tgatgatttt
     3301 agggattatg gcggagtgtc taccttagtt caaatattga ttaactctga tagaaaaaca
     3361 gaaaagaata ttttgggaac gattgaagaa caagacccag ctttagcagc tcaaattaaa
     3421 tctcatatat ttatctttga agatattgtc aagctagatg atataagtat acaaaaagtg
     3481 ttaaaagaag taactatgaa agatttagta tatgccttaa aaggtgtgga tgatgcgatt
     3541 tcagatgcta tatatagaaa tcaatcttct agggcagcag atgcacttag agaagaaatg
     3601 gaaatggttg gtgttataaa ggttacacag gttgaggctg ctcaacaaaa aatagtaaat
     3661 agtgtaagaa aacttgaaag agaaggcgcc ataactattg taaggaattt tggtgatgac
     3721 tttgttgaat aataagatta ttaaaagtaa aaaagcatca tataaaggag ttttagattt
     3781 atccataata gataaagtta gagtgggaga atcaaatcta aataaagaaa atgattgtag
     3841 atttagggat gaagtaataa aggaagctat agaatctgca gagataaaaa agaatgagat
     3901 attagaagaa gcaaaaatga acgcacaaaa tatagaaaag caagcatatg aaaaagggta
     3961 tagtcaaggt aaaaaaaatg gatatgagga tggatataaa gagtcatatg atgaatatac
     4021 ccaaaaagct aaggatgaag cagattttat aaaaaatcag gcaaatttaa tcttattaca
     4081 agctaatgag aagatgactg agtacataaa agaaaaaaat aaagagatag taagacttgc
     4141 tattaatatg gccagttctg ttttaaaaaa gcatttcgaa gatgaagaat ctatgagtga
     4201 tttgttaaag caaattattc aagattatga aggaagtagt tcatttatag taagatgtaa
     4261 ttctttctat aaagataata tagagaaaga atttaaatta tttaaagagg aaacatcact
     4321 aaaatcaagt gtatttgtta ttgcagatga tagtatagat aaaggaaatg ctgttatcca
     4381 aaaggaaaat ggaagaataa tagttggtat tgattgtgct atggaaaaat taaaagaaga
     4441 attgcttggt gaataaagta tgtataaaac tgatatatat atggattttg acaaaataaa
     4501 taagaaaata gaaaatttag aactcactat atcagaaggt agagtaaaga agataatagg
     4561 acttactgta gaagttgaag gaataaaagc ttttgttggg gagttatgcg ttatatacaa
     4621 ccaagttaat aaaccagtga attgtgaagt agtaggtttt aaagataaag aagtcatatt
     4681 aatggcgttg ggagagttaa cattgatagc tcctggttgt aaagttattt ctaaggggat
     4741 acctttaagt gttatgtgta gtgataattt attaggtaaa gtacttgatg gtcttggaaa
     4801 tcctatagac aactcagatg ctgttttagg agatagatac aacttgaaca atgaacctcc
     4861 tgacccaatg aagagaaaaa agatacgaaa tataatggaa acaggtgtaa gagctataga
     4921 tgcatttact acttgtggtg aaggacagag aatcggcata tttgcaggaa gtggagtagg
     4981 taagagtacg actctaggta tgattgctag gaatgcaaaa gcagatgtaa atgtaattgc
     5041 tcttattgga gaaagaggaa gagaagtact agattttatt gataaagatt taggtgaaga
     5101 gggaatgaaa aaatctgttg ttgtgtgtgc aacatcagat aaagcccctc tggttaggct
     5161 aaaaggtgct cttactgcta ctgcaatagc agagtatttt agagaccaag gcaagaaagt
     5221 aatacttatg atggattcag taactagatt tgctatggca caaagagaag ttggtatagc
     5281 aataggagag ccaccagccc aaaaaggata tacaccatct gtatttgcca ttttgccaaa
     5341 acttatggag agaacaggga catcagataa aggttctata actgcatttt atacagtatt
     5401 agttgatgga gatgacttta atgagccaat agcagataca acaagaggga tacttgatgg
     5461 tcatatagtt ttatctagag acttggcaaa taagaatcac tatccttcta tagatgtatt
     5521 gaacagttta agtagactta tgaatgagat tgcttcaaaa gaagacataa aaatagcttc
     5581 ttttgcaaga gacatgcttg ctgagtatag agaagcagaa gatttgataa atataggtgc
     5641 atatgcttca ggtacaaata aaaaaataga tgaagcaatc tattatcatg aacagattat
     5701 taattttttg aaacaaggga taaatgaaaa gagttctttt aatgaaacta caagtagttt
     5761 gaaaagaata tttgaataaa taatttaaaa aaatatttga ataggttgtg atggaattgg
     5821 ataaaaactt taagtttaga ttacagaaag ttttagactt aaaaatgaag gatgaagaaa
     5881 agataaaaat ggaatttgct aagattcaac aaaaaaaaat agatatagag actaacttag
     5941 aaaatttaga aagtaattat agcaaatatt ctataagtaa aaataacgat agtattcaag
     6001 aacaaaaaat aactattaat tatttgttag ctttaaacaa ctctataatg gatttatcag
     6061 aggaactgga caagtcaaca aatgaacttg agaaagctag aaaacagctt atagacaaac
     6121 agatagaaag aaaatcatta gagaagttaa aagaaaaaaa atatgggcag tattataaag
     6181 aagaaaattt aaaagaacaa aacactaatg atgagtttgc aagtatgagt tatttaagga
     6241 atagacaagt tctataagaa tagtaggaag tgaggatatt attgtatgaa tatacaaaca
     6301 gattcttata atgttattaa ggacattggg gataaaaaga gttcatctaa gagtaaaaac
     6361 gtaaaaaata ataagtttga agataatcta tataaggcaa cagacaataa acaagttaaa
     6421 ggtagaaaag aaatgagtaa tcaaaatatt aactcaaata aagaggaaaa tagtgaaaat
     6481 gagtgtcatt tagatgaaga taaaagtatt cacttagaag atgatagtac atatgaaaaa
     6541 gactttagta agaataatga gattttacaa caattactga atctgttaaa aaataatgac
     6601 aaaaaagata aagatataaa ctctatatta aatgatttag aaaaactaaa tatatcagat
     6661 gaattaaaac aagaacttaa acttaataaa aatgtattga ataagattgt aaaccaaaat
     6721 gctaaactaa acaatgctaa tataaataac tctgatgaca tgcaaagttt agacatgatg
     6781 aagttaagag aacttttaga taatgatgct aataagaact ttgaagtaat tagtaaaact
     6841 acagaaaatg atacaaaaag catagacttg tacaattttg atagtaatag aatggataat
     6901 attaactcaa ataagcaaaa agatgatagt tctgatattt tggaaaaact agcagggggc
     6961 aatggaaata agagcggaaa ctttggtcaa gtgataaata aaaatcttga tacgaataaa
     7021 gttaaaaatg ataatgtcaa tttaaaacat atagaatcaa atgtaatgga tgattctata
     7081 aaagctatta agcatatgaa aacaaatgat atacaagaat taacaattaa attaagacca
     7141 aaagaattag gtgatatgaa catacaactg ctaaaagatg gtgaaaatat gagagctgtt
     7201 gtaactgttt ttaataaaga tgtatttgat tctataaata aaaatatgac agatttaaag
     7261 caacatcttg aacttacaaa tgtaaacata aaagatgtat cagttcagat gcatagtgat
     7321 aatagaaata catctgatac ttttgataaa gcatttgaac aacagaatag acaaaataac
     7381 caagaaaaca tgaacaagca gaatacaaat aataagcgta acaatatggc catagaagat
     7441 gagattaaag aaaatattat tgatgatgat agagtagatt tattagctta gtaaggggga
     7501 agtattgtat gtacggagta gcaacagctt tggcaggtgc tcaaggaagc tataggtcaa
     7561 ataacttaag ccaacttgaa agaaatacta atgacattgc gaataaacaa gttagaagca
     7621 aaggaaatca gacattgaca gataatggaa ctccaattat aggacagggt gatgatgctg
     7681 ataaagattt atttatgaaa atacttgtag cacaaatggg taatcaagac ccaatgaatc
     7741 cacaagaccc aacagagtat gtatcacaat tagcacagtt tgcaacttta gaaaagatgt
     7801 ctgctatgaa tgaaaagcta gatatacttt tagctttaag caactctact ctaataaatt
     7861 ctgctttaag catggcaaca tcattaatag gcaaaaaagt agaggtatct ggagaagaga
     7921 aagaagatga aaacattgta ggtgcagtaa agtccacata cataaaagat ggagaggtac
     7981 acttagagct gaaagtagaa ggcagtgata aaactgtaga tgttaaatat gaaaaattac
     8041 ttaaaatagg agaagtagat acaaattcag atgaagaaga aaattctcca gaatcaggtg
     8101 aagaagaaag ttctacagaa caagtataat attaaactag aaaagaggta tattcatgat
     8161 aaaagcaatg tattcaggtg taagtgggat gaaagcaaat caaacaaagc tagatgtaat
     8221 aggtaacaat gtagcaaatg cagggactac ttcttttaag aaaagttctg ctagaataac
     8281 agattcattt tatcaaactg tattatatgc aagtgctcca acagctgcat taggaggaac
     8341 aaacttaggt caagtaggtg taggtagtaa gatatcaagt ataaataaag atatgacgca
     8401 aggaaatgta cagccaacta atagaccatc ggatttaatg atagatggag atggatatct
     8461 tccagtagta aggcaaggta cagttatgta tacaagagat ggctctttta acttagatac
     8521 aggatttaaa gcagcagctg gacctttaaa agatgtcaaa ggcggaagac ttgtaaatgg
     8581 tgatgggtat atgttacaag gtatgattta tgagggagaa tataaagcag atacaggaga
     8641 atttgttaaa aaaggtgatg ccaaaggaaa agaacctatt gtaatacctc ttcaaagtat
     8701 aagtcctaaa gatggaacag ttcaaaatgt attagagtat aacatatcaa aggatgggac
     8761 aatagaattt ttattgagtg atggtcaaag aactcagttt gtgtataata atattcctga
     8821 tgatgctaag gtaggagata cacctgctgg aatcgataaa ttaaagggaa ctattcaaaa
     8881 agtacaaaca tatgcattcc aaaatccagc aggactagat gcagagggag gaaacttatt
     8941 caaaccatca gcaaactcag gagacccaca aatagcagga gctacaggaa caataataca
     9001 aggggcaata gaagcttcca atgtagatat agcagaagaa tttacagaaa tgataatagc
     9061 aagtaggtct ttccaagcaa acagtaaaat aatatcgaca tcagacgaga tacttcaaga
     9121 aatcataaat cttaagagat agtaggtaca tattatgata agagtaacgg atttaaacgg
     9181 aagaaaatac atattaaatt cagaccttat agtgaggatt gattctgtac cagaaagtag
     9241 gattctcctt acaacaggag aaaaaaacct tgtaaaagag tcggtagatg agatagtaga
     9301 taaagtaata aagtataaaa gaaaaataaa tgtaggttat attaggagcg aaaaataatg
     9361 aaaaaaacag attcactgac atttattgga ctaatagtat ctacagtatt agttttagtt
     9421 ggtgccgcaa aaggaagtag ttcagggtta aagaattttt ttgatgtttc atcaatatta
     9481 atcacagtgc ttggttcatt tggagctcta atgattacat ttactataga tgatataaaa
     9541 cttataaaaa atgcattaca atactcattt aagacaatga gtgtatcaaa gttagattta
     9601 ctagaacaat ttaaaacact ttctaaaaaa gctagaaaag aaggtctttt atcaatagaa
     9661 agtgatgtaa tgggaataga agatccattt atgaagaaag ggttagagct atgtattgat
     9721 ggattggaaa tcaatgagat aaggtccatc ttagaactag agatagattc tgttgaagat
     9781 acaaataaca gatactctaa actatttaaa acttggggaa cctttgcacc tgcctttggg
     9841 atgttgggaa cactaatagg tttaatacaa atgttagctg accttacatc tccagattta
     9901 atagcatctg gtatgggaaa ggcattaatt acaacgtttt atggttcttt acttgccaat
     9961 atcgctttga acccaattgc atataatata gatgagaaga ctgaaaaaga aatttatgta
    10021 aaggaaatga tgttggaagg tataataagc atacaatctg gggagagcag tatagtagtt
    10081 gaggaacgtt tagccacata cctttcaaac aatgagaagt tagaaattat gaaatccaat
    10141 aaaaatacag aaagagcaat gagcaatggc gcgtaaaaaa aataagaaaa aggatgagat
    10201 aaatccagat gcatggctgg ctacttatgc agatactata acactgattc ttacattttt
    10261 cgtattattg tatgcaacat cagttgtaga tatggaaaaa tttaaaaaca tagcagatgc
    10321 gttaaaaggt cagttttctg ggacaagtat atttagtacg ggttcaagta caagtgagaa
    10381 atctccaata gacagtgttt tagtggataa aaatgataaa tcaaatttag aacaggattt
    10441 aaaagataaa gttaataatt ctaacttaca aaatagtgta acagtcaaag atgatagtag
    10501 agggatttta ctagagctag atgattcaat tttatttgac tctgggattt ctgagttaaa
    10561 agtagatagt aaagctactc ttaataaagt atatgacatg ataaagatga tgaaaaataa
    10621 gatagttata gaaggccata ctgataatgt acctgtaaaa tcaagtacac atgaatccaa
    10681 ttgggattta tcttctagta gagcagtaag tgttgtaaga tactttgtag aagaaaaggg
    10741 catggaccct aagctatttt cggcaacagg atgtggagag tatcaacctt tggtttcaaa
    10801 tgaaacagca aagggcaggg ctgagaatag aagagttaat atacttataa ctattgacta
    10861 ggaggaagag gtttgttcaa aaataaaaaa attattataa ttattttgat actgattatt
    10921 ttctcagcgg gagcaggttt ttttatatta ggagaaggtg gaaataaaca aattgacaag
    10981 attgcaggtt tgtttattcc aacaggaaaa ggtaaagatg aagtaaagac cgaaaatctt
    11041 gacttagaag agatggtatt aaagctagct gatgaaggtg taagatattt aaaagtatca
    11101 ataactgtat cttatgattc ttcatacaaa gatatagaaa aaaatacgcc tttaattagg
    11161 gataatatta taaattgttt tatggcaaaa agagcagatg attttactgc tgagaattta
    11221 aataacataa aagaagaaat taaaaataca ttaaatatac aactaggtga aaatgtaatt
    11281 caaaatatat atttcacaaa tatagtagtg caataaaagg atggtaagac atgatacttt
    11341 atttactaaa aatattaata tgtatcccaa taattttaat attgatagtc ttatctttaa
    11401 aaattagcca aggaacttta ttaaaaaaat ataatgataa atatgtaaaa atactagatg
    11461 ttgtaaatat aagtaaaaac aatagcataa taattttaaa aataggtgaa gaaggatgca
    11521 ttatggcatc ttcttcctct agcataaata aaataaaaga tttgtccaaa gaagaaatat
    11581 taaaaataga aaatgacatt aaagaaagaa attttaaaat tcaagatttt aaaatggaaa
    11641 gaattaaaaa gaaatatgat ttcagtagat atattggaaa atttacaaaa aaggaagata
    11701 agtaaatgga actattatca aaattacctt tggctgatgt accatataca agttcaatgc
    11761 aattgttttt atttctaact gcattaatgt ttttaccatt tgtaatgttc atgatgacaa
    11821 gctttgtcag aatagttata agtttatcat ttttgaaatc agcattagga gcacagcaag
    11881 caattccaag ccaaattttg gtagggcttg caatagtatt gacaatattt ataatgagac
    11941 cagtattaaa tgagataaac gaaaaggcgt tacaaccata tatgaaagaa gaagttacta
    12001 tggaagaagc tatgaaggaa gcagaaggac ctattaaaga gtttttatta aagcaaacca
    12061 gacagacaga tttagattta tttgtagaac aggctggttt aaaagaaaag aaattaactc
    12121 gtgaaaatat accactatct gttgtagttc cagcatttgc tataagcgaa ctaaaaactg
    12181 cttttcaaat aggtttttta atatatatac catttttgat aatagacctt gttgtggcaa
    12241 gtgttttgat gtctatgggt atgtttatgt tgcctccagt catgatatca ttaccgttta
    12301 agttattatt atttgtaatg gtggatggat ggaatttgat agtaaaaaca ttgatattgg
    12361 gatttggata ggagaaaaat agtatgagtg aatttatgat aatgagtgtt cttaaaaatg
    12421 gcatgtttac aggagctatg attgcagctc cagtactcat tgtttctctt gtagttgggc
    12481 ttttaataag tgtgtttcaa gctacaactc aaatccagga gcaaacattg acctttgtac
    12541 caaaactaat agctattctt ttagtagtag ctattggtgg aagctggatg ttacatactc
    12601 ttattagctt tacctctgag atattcaata tggtatctct tataactaaa taagtagggt
    12661 atatatgatt tttgtattta ttagaatagt agcattcttt ggaacactaa gaatactatt
    12721 tccagctggt acacctgcgt tatttaagtc actctttgca atcattatat caatattgat
    12781 ttcaagtact atgcaaattg aatatgcaac aaatatagat aacattgtat tgtttacctt
    12841 atatggtata aatgagacca taactgggat tatgcttgga tatataacta acctatgttt
    12901 ttatagtatc agaatggcgg gttctatgat ggaccaacag atgggattat caatgattaa
    12961 tatgtttgat ccaaactcac tgactcaaac aactttaatt gataatctta tgaactggac
    13021 agcacttatg atatttttta gcatggatgg tcatcatgtt ttaattcgag gtataaggta
    13081 tagttttgag ttaattccaa tagggaaatc attcatagat aataatatag attatataat
    13141 aaacatattt gtgcagtgct ttttaactgg attcaaaata gcaataccta ttgtactttg
    13201 tctgcttatg gcagatttta ttttagggct tatatcaaga agtgtacctc aattaaatgt
    13261 aatgatagtt ggtatgccat taaaaatatt ggttggtatt gcattattta tcatatcaat
    13321 acctttaatt gtcaatcaaa taagtcattt attaagtcaa ataccaaaga tgtatgaggg
    13381 aacttttgca ctagctccta tgttttttat gggttcagca gataaaacag aagaagctac
    13441 tccaaagaaa aagggagaac aaagaaagaa aggtaatata gcaaagagta gagaattacc
    13501 cgttgcaatg actctacttg cattcacctt attagtacct acattatttt catatgtagt
    13561 agatacactt aaatcttcgc ttaattattt tttgagtcta gatttttata tgaacataaa
    13621 ttattcaaac ctagagaaat tagttatagc aggattaata gattttttta agatattttt
    13681 accaatagct attccctttt tagttttagg cataatagca aacctatttc aggtgggaat
    13741 actgtttact ggtgaaactt taaaaccaaa tttatcaaaa ttaaatccag ttaatggatt
    13801 caaaaacatg ttttctatga gaagtttgtc aaccttaata aaggatacag cgattatatc
    13861 tattttggct tacataggat atacattctt ccaagataat tatttagata tattaaagct
    13921 aggtaatata tacctaccaa ctttaatgta cacagttaaa gacttagtat attcaatatt
    13981 gagtaagatt tgtgtagcta tgatagctat agcagttgca gattatgtct atcaaagata
    14041 tagtcataaa aagcaactga gaatgacaaa gcaagaagtt aaagatgaat ataaaaactc
    14101 agaaggagac ccagaagtaa aagctaagat aaaacaaaaa caaagacaaa tttcgtctca
    14161 aaggacaatg caagcagtac caagtgctac tgtaatagta accaatccaa cccacttatc
    14221 tatagcagtg agatatgaaa aagggaaaga ccaagctcca gtagtggtgg caaaaggtgc
    14281 agattattta gcttttaaaa taagggaaat tgcaaaggga aatgacatcc ctataataga
    14341 aaacaaacct attgctaggt tgttatataa gcaagtagaa atagaccaag aaattccaga
    14401 ggacatgtat caagcttttg cagagatatt agtagcagta tataaaatta aaaatagata
    14461 caaagtcccc aaaaggtagg ataagtaatg aataagttta atttaaagga aaatatggat
    14521 gtaatagtgg catttggtgt tgtagggatt gttcttatga tgatagtgcc attacctaca
    14581 tttatattag atattttact ggcaattaac ataagtttat ctgtgctaat tttactaatg
    14641 acattattta caacaaatgt acttgattta tctatatttc caacagtgtt acttgtgaca
    14701 acactattta gactgggatt aaatctttca tcaactagat tgattttaac acagggatat
    14761 gcaggtgaag ttatagaggc atttggaagt tttgttgtag gtggaaatta cgtagtagga
    14821 ataattatat tcttaattat attaattgtt caatttgttg ttataactaa tggttctgga
    14881 agagtatctg aagtaacagc tagatttacg ttagatgcca tgccaggtaa gcagatgagt
    14941 atagatgcag atttaaatgc aggagttata gatgacaaaa ctgctagaaa aagaagaaaa
    15001 gagcttcaac aagaagcaga tttttatggt tctatggatg gagcaaataa gtttgtaaag
    15061 ggagatgcca tagcaggtct tatagtaaca gcaataaaca taataggtgg aattgtaata
    15121 ggagctgtaa tgctgaatat gactcttatg ggtgcagcgc agacatatgt aagacttact
    15181 ataggggatg gtcttgtaag ccagatacct gctctattaa tatctacatc agcaggtata
    15241 atcgtaacaa gagcttctac tgatgaaaac tttggtggat tagtaggtaa acagttaacg
    15301 gcaataccaa aagcagtaat gatgactggt tgtgttctaa tcgtacttgg tatgatgcct
    15361 ggattaccta aaccagcatt tttttcacta ggtataggag ctatagcagc aggttacttt
    15421 ttaggaagag aaaagaaaga tactttagaa gacttggagt ttgaaaaacc tgatatggta
    15481 aatccagata aaaatgaagt agaagatgta acaaacttaa taaatgtaga atcaatagaa
    15541 gtagaaattg gatatggttt aatatcttta gctgatgaat cagcaggagg agatttactt
    15601 cagagaatag catcaataag aagacaatgt gcaatagaca tgggtatagt cgtacaaccc
    15661 ataagaataa gagataactt acagttaaat ccaaaccaat acaatataaa aataaaagga
    15721 aatgtagttg gtgggtatga gataatgcct aatatggttt tatgtatgga ccctatgaat
    15781 caaggattta gtatatttgg aataaagaca attgaaccta cttttggaat agaggcactt
    15841 tggatagaaa gtgacaaggt agaagatgca gagttaaagg gttatacagt tgtagattca
    15901 tccacgatac ttataactca cttactagat ataataaaat caaatgcaca cgaattactg
    15961 ggaagacaag aagttaagac tattatagat gcagcaagag aaaattatag tgctgtagta
    16021 gatgaattga taccagattt aatgacactt ggtgaaattc aaaaagtact taaaaatctg
    16081 ttaagagaaa aagtaaatat aaaggatagg gttacaatac ttgaaacgct tgcagaccag
    16141 tctagaaata caaaagatat agagcttctt actgaatatg taagaatagc cttggctaga
    16201 agtatttgta ataatttagt tgatgatgat aagacaattg tagtggctac attgtctcca
    16261 gaaactgagg agttggtatc taataattta caaaggtctg taaatggaac ttatccagca
    16321 gttgacccag aaaatacaaa taaaatattt gaaagtatac aagaagtaat gagcaatgta
    16381 tatttcaatc ataatatacc agtaatagta gtatcaccaa agataagagc tccatttaga
    16441 aaattagttg aaattgtata tcctaatttg actgtattat cattaaatga aatacctaac
    16501 gatattaaaa tcaaagcaga aggtgtggtg agcatatatg atgacgaaaa agtatacagc
    16561 taacacaatt caggatgcta tgaatctagc caaaatggaa ttgggtgaca atattacatt
    16621 aatagataaa aaagaggtaa gaaagtctgg aatacaaggt atctttttaa agaaagatat
    16681 tgaactaact attggatggg aaaagagaga aaacgtagaa caaaaagatt taaaacgaga
    16741 gatagaacaa ttaaagtcaa ttattaacaa tatggggttt gataataaga atgatattga
    16801 tatagataaa atatgtaaaa atctactaag tttagaatta aatgaagaaa ttgtagaatc
    16861 tataagaatt gatttgcaag aaatgaagtt caatggaatt gatacaagca aaaatttagt
    16921 agaaatttta aagaaaaaga ttaaaataga aaatcaagca ataaatggga aaattgctct
    16981 agtcggacca ccaggagtag gtaaaactac tacaatagca aagttagccg caaaattagt
    17041 ttttgaagaa aataaaaaag taggagtaat aactatagat acatacagga ttggtgctgt
    17101 agagcagttg aagatatata ctgatataat gaatattcct tttaaagggg taataagtcc
    17161 tgatgaaatg gaattggcac ttgatgagat gaaagattgt gatgttgtat taatagatac
    17221 aacaggtaga ggctataaaa actctatgca aatactagaa atcaaaaatc ttatagataa
    17281 agctgaaact gataatatac atttagtttt gaattgtaca acaagagaaa gtgatacaaa
    17341 agcaattata gattcatata gaaatgtgaa ttttaaaagc ttaataatta caaagctgga
    17401 tgaaacaata acatatggct caatatttaa catagtgaat tatgcacaaa aaccaatatc
    17461 atatattaca acaggacaaa atgttcctga tgatattata aaacctaatg aagataagat
    17521 tataaggtta ctattaggag ttgaaagtat atgatagacc aagctcaaat tttgagaaaa
    17581 atggcaatgg aaaaaagagg ttctgatgag attatagtag agaatgagaa tacgcctaag
    17641 ataattacaa tagcttctgg taaaggcggt gttggaaaga gtaatctcgc tacaaatttg
    17701 tctatatgct tgaccaaact taataaaaaa gttcttatat tagatgcaga cataggaatg
    17761 tctaatatag atataataat gggagtaaat gtaaaaggga ctataattga tgtaattaat
    17821 ggtgaaaaaa atatagaaga tataatttca cagactaagt atggtgtaaa tataatatct
    17881 ggaggttctg cccttaatca tatagaagac tttacagaag ctcaaagaaa taagtttata
    17941 catagcatag agcagattca cgatgtggat tttataataa ttgatacagg agctggtatg
    18001 agcaaaagcc tattgtcatt tatatactgt agtactgaat ttttcttaat aacaactcca
    18061 gaaccgacat cattgactga cgcatatagt ttattaaaag ctataagtaa ttttggcata
    18121 aagagaagtg caaatataat aatcaatagg gtaattgatt tagaagaagc aaaatctaca
    18181 tacaaaagaa taaagatggt tgtggataaa tttctaaacc ttaatctgga attgtatgga
    18241 tatatagtag atgacaaaaa agtaggttta tgtgtaaaaa aacagatacc attcatttta
    18301 gaatatccta caagtattgc atcaaaatgt attatagcca tagcaaagag aatggtaagt
    18361 cagtcaagag aagatattgt tgataataaa agttttctaa gaaagatgtt tagtatattt
    18421 aaaagctaag gaggcgtagt taatgaatag agaagaatta ataaaagaga atatgcctct
    18481 tgtaaagagt atagcaagaa aattttttat accagggaaa ggttttgaat atcaagactt
    18541 agtaaatagt ggagtcattg gattgattga tgcaataaat aaatttgatg ttgaaaaagg
    18601 ggcaaagttt tctagttact cttatataaa gataaaatct gctatattag atgaaattag
    18661 aaatcaaagt ccgatttcta aacataatct aactaaagta aataagtata atagagtggt
    18721 agaaaagttg caatctaaat tattaagaga gccaacttca tatgaaattg caaaagaatt
    18781 aaaggtttca gaaaaagaac ttcatgatat cgaaagtaat atagacatgt taaatattgt
    18841 atcattaaac tatgtaatct ttgaagatac aaatgaaact gtacaagatg taataagtga
    18901 tagagaagag gaagctccag aaaatataat agaagaagaa gaaaagttag agattctatc
    18961 taaagcaatt agtaatttaa atgaaagaga aaaattaata ctttcactgt actattatga
    19021 agatttaaat ctaaaggaga tagggaaagt gctaggtgtt tcagaatcaa gagtttcaca
    19081 actccacaga aaatcaataa gaaacttaag aaacaaaata aaagaactta aatattctat
    19141 atagatggtg attgataaga tgatacaaat tatattaata gtagtgtcta ttttaataat
    19201 agtaactata gttataaatt tacataagga atcaaaaaat aaagataaca gatactttga
    19261 taaaatattt gagcaagaat atgaagcaaa tgatagatta ataattgcag aaaaaagaag
    19321 attcttttta gacaaagtac ttgctgaaat aagaagtaat aatcatataa aagtttatga
    19381 aaattataac ctaataaagg ctaatgaaga aagaaaggaa agcatattta tagaatcaaa
    19441 aaaggaagta agacaagaaa atgagttatc tcttaaagtg aaacatctta taagttcagg
    19501 ttatgatgat gtggaaatat gtaagatgct agatattgga aagggggaac tgtcattaat
    19561 aaggagttgt tacaaaatat aaggatatta ttatgtgata aaaaagtatt cttaggaata
    19621 ggtattggta tattaatatc aactttatgt ataatgcttt ttagtaataa tgatatgagc
    19681 aaggcagaga tagaaaaaaa ggccagagcg ttaggtatga aatatacaag tgagatgaaa
    19741 gttttagaat agatgacact gtttaaacat ttacaaaaaa ggaggtcata atgtgataag
    19801 aggattatat acagcagttt ctgctatgat aactaatcaa caaaagcaaa atgttgttgt
    19861 caataaccta gctaatatgg atacaaatgg atataaaagt aaacaacttc tgactaaaag
    19921 ttttgatgaa ttgaagttgg aaggttacaa taactacgca aatggaacta aacaaaagca
    19981 ggttataggc gatataagtc caggtatttc tatggatgaa actatcacaa actttaatca
    20041 aggacctata aaaactactg ataataaaat ggatgtagct attcaaggta agggattttt
    20101 tctagttagt gatgcagctg gaaatcaatt ttacactaga aatgggaact tcagaacaga
    20161 caatcaaggt gaccttataa caagtgaagg gtattatgta cttgggacga atactgctac
    20221 tggtgctgta ggtcatataa atgtaggaaa tcaaaaattt gaagtggcaa aagataatac
    20281 aatatcacta gacggaaatg ctatgtataa atttaatatt gtagatttta ataattacaa
    20341 taacttaaaa aaagaaggag ataacttgta ttctggagga ggagctatga aagtaaataa
    20401 tgcacttaca atacaaagtg cgatagagtc ttctaatgta gatatggtat ctgaaattaa
    20461 taatatgatg acaatatcaa gagaatatga ggcaaatcaa aagataattc aggctatgga
    20521 ttctaaatta gcaaaaatag caagtgagat tggttcagta agataggtaa taggaggtga
    20581 atttttatgt acacaatgtt ttatactagt aaaacagctt tatcagcaaa tcaaagtaaa
    20641 ctagatataa tatctaacaa tatagcaaat tctgattcga caggatataa aaaaataaat
    20701 atggaatatt ctgatttagt ggatgaagca ctaaatagac catcatatcc tacaaatggc
    20761 aatgatatat cgactggaac aggagctaag gcaacagctc ccatgagaat ttatgaacaa
    20821 ggtgcattgg ctcctacaga tagtaagagt aatttggcga tagatggaga aggattcttt
    20881 agagtgataa gaaaagatgg tacatatgca tatacaagaa atggtggatt taatgtagat
    20941 gctttaggta agattgtaga tgataatgga aatatattag atgttcaatt tgaggcagga
    21001 tataactata ataatacgaa tatagatagt acaaacctga cagtttcaag aaatggagag
    21061 ctattcgtag ataataaaaa aataggaaaa atcaacttat atcaacctat aggaactcaa
    21121 aattttatat ctgaaggaga tagtttattc gtcgcaactg atgcagcaca gattaaacaa
    21181 gttgagaaag tagatatagt tcaaggctat agagagaggt caaatgtttc tttacaagaa
    21241 gaatttgttg acttaatagc cactcaaaga gcatttcaaa tgaactcaaa gggaataaag
    21301 acagcagatg aaatgtggca aatagctaat aatttaagag ctaaataatt atgtaaggtg
    21361 aggaatattc atgaatgata tgaaagagta cgattttggc aaacctcaga gttattcaag
    21421 agaaaattta acttgtttaa attttttatt agctgaattt tgtaaaaaat ataaaaacta
    21481 tataatttat gaacttaagt gtaattctag tatggttgtt gatagtatag accaagtaaa
    21541 ttatcagact tttttagata gagtaacttc ttcatgtgta gtagtacaaa atgcaatgga
    21601 acctgttatg aaaaactttt catttagaat agatagaagt gtagctgata tgtggattga
    21661 tataacttct ggaggaactg gagttgtaag agataatgat agagagctta cagaacttga
    21721 taaaaaagtg cttttacatc ttatggatga tttagtaaga aatatgcact tatttgaggg
    21781 ttttgaaaat atacaagtat tggatacaca tacacatatt aatttgccac aattatgctc
    21841 tccaactgca ccagtatgcg ttgtagattt aaaagtgcta aatgataatg agtatattgg
    21901 aagtgtttct ctttgttttc catacaatgg tttagaggct ctattagaaa gtatatctgt
    21961 tatagaattt tttgataatg atgtaggaga tgattcagag gaatttacca aaaaaatcta
    22021 taatagtgta tctaatatag atttatcagt tattgcagag ataggaaaag tatcaattaa
    22081 tgttgaggat ttacttaaac tagaagttgg agatgtaata gtaacaaata aaaaaataaa
    22141 tgattatata gatatatttg tagaaaattc aaaatcatac acagcaacac caggatttat
    22201 atcaagcaaa aaaggtgtaa aaataaatga tgctgttgga aaagaggtgt agttatggac
    22261 aatagtaatc ttatttcaga tgaagaagca aagatgttat taaatgataa agttgaaagt
    22321 gaagttgatg gagatgatga gttagcctta gatgtctctg aagttaatcg aaataatata
    22381 caaaggatac ttgaccttaa gttagaatta tctgtggtta taggaagaac taagatgact
    22441 ctaagagata ttttaaattt acataaaggt tctcttattg aactggatac tttagcagac
    22501 caagatgtgg aaatactaat agataataaa gtattggctt atggaaaagt agtggtggta
    22561 gatttaaatt ttggtgtaaa gataacaagt atagtaagtg aagaagacat ggtaaaatct
    22621 cttgtttaga gataacatta attaatactt aaggcatgag gtggtactta ttttaaactt
    22681 aaatgatata aaaaaagata tattaaaatt caataaaaaa gactcatgtt ctctatataa
    22741 tgaatctatt aataatataa ataataattg tatatcaact ggattgaaat ttttaaaaca
    22801 agcaagagat ttaaacccag atgatgttga tatattaaat ctcacaggtc ttgtaaactt
    22861 actaaaatgt aattttgatg atgctgtaga aagtttctat aagagttcac tttgtgaaaa
    22921 aactgctctg tgtgaaaagt atataaacat gcttacatct gaggagtttt taattttctt
    22981 tgaaaagtac aatcaactta taaggttaat aaacaaagga atgtataaag aagctataca
    23041 agggtttaaa cttataactg accaatactg tgatttaata gaaccttatg agttgcttgc
    23101 cttactgtat gacaaggaag aagaatatat aaaatttgat gaatgtttag aagtattgaa
    23161 gattattgat aaggaaaatt atttattaaa tcaagaaaat aacatattag atatgtataa
    23221 tagtgctaat atacaaacta aaagtaatat aaaagtagat gattataaga atgagttttt
    23281 acataaaagt agtaataatc ataagattaa tgattataat aatgtaacta aagaaaagag
    23341 ttcaagcaaa aaccagaaaa gttcagattc taataagaaa aaaagtatta aaaaaaataa
    23401 gtacctatat atagtcataa tattaggaat attattagta ggacaaagta tatatagttc
    23461 ttataagatg aataatttaa ctgataaaat atctaaaaat aatgaaacta aaaatgaagg
    23521 gtcaatagat tctagtttgg ataagaaaga aaataatata aaagaaaatg aagatatgaa
    23581 taagacttct aatatcaata agagtaataa aaatattaat aaagaggatg taaaatctaa
    23641 aaaaaatact gtagaaaatg aagatgataa agaatctgat ttatttacag aggatgaatt
    23701 gatgtctaag gcaaatactt taaaatcaga aaagaaaact aaatcttcta tacaactata
    23761 taagaaagtt gcagatatag gtgaaaataa agggaataca tctgaagcaa cttatcaagt
    23821 tgctattctc agtgaaaagt taaaagacta taaaactgct gaagaatatt ataaaatgta
    23881 tgtagaaaat tactcagaaa aagatgctta ttttgatgaa tcttattata atttaggtat
    23941 gatgtactat aacaatggtg atttaaaaag ttctaaattg acatt
//
LOCUS       DAEMAE010000040        23757 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEMAE010000040 DAEMAE010000000
VERSION     DAEMAE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.041"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(48..5105)
                     /locus_tag="KPH93_001275"
     CDS             complement(48..5105)
                     /locus_tag="KPH93_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG1688106.1"
                     /translation="MLERTRVEKNPKIIEIFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKTGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     CCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFNARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTDRQELCNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSHSSSKLNKIISNISLSTCEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENANYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRQTSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRS
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKNISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEDGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELTSIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIISKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(5130..5549)
                     /locus_tag="KPH93_001276"
     CDS             complement(5130..5549)
                     /locus_tag="KPH93_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688107.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKAIINFLTNEEIESQADIGKVGFGSIYNENKESVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDYPINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(5783..6034)
                     /locus_tag="KPH93_001277"
     CDS             complement(5783..6034)
                     /locus_tag="KPH93_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688108.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            6123..6854
                     /locus_tag="KPH93_001278"
     CDS             6123..6854
                     /locus_tag="KPH93_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG1688109.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFNKLNDVYKSLPAGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKVLDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSERLSFFDELMTLDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLI"
     gene            6913..7824
                     /locus_tag="KPH93_001279"
     CDS             6913..7824
                     /locus_tag="KPH93_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1688110.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            7875..8687
                     /locus_tag="KPH93_001280"
     CDS             7875..8687
                     /locus_tag="KPH93_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG1688111.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            8797..9489
                     /locus_tag="KPH93_001281"
     CDS             8797..9489
                     /locus_tag="KPH93_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1688112.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            9576..10517
                     /locus_tag="KPH93_001282"
     CDS             9576..10517
                     /locus_tag="KPH93_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688113.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDKKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKENNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNTETNNIN"
     gene            10554..11711
                     /locus_tag="KPH93_001283"
     CDS             10554..11711
                     /locus_tag="KPH93_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG1688114.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            11863..12423
                     /locus_tag="KPH93_001284"
     CDS             11863..12423
                     /locus_tag="KPH93_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG1688115.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            12451..12942
                     /locus_tag="KPH93_001285"
     CDS             12451..12942
                     /locus_tag="KPH93_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688116.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            13171..13722
                     /locus_tag="KPH93_001286"
     CDS             13171..13722
                     /locus_tag="KPH93_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688117.1"
                     /translation="MQSLNEIIAENLKKIRKEKRLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            13808..14740
                     /locus_tag="KPH93_001287"
     CDS             13808..14740
                     /locus_tag="KPH93_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG1688118.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGKSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKIGEKLNVPVFKHKILKNPKDLPIW"
     gene            14795..15658
                     /locus_tag="KPH93_001288"
     CDS             14795..15658
                     /locus_tag="KPH93_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG1688119.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            15905..17770
                     /locus_tag="KPH93_001289"
     CDS             15905..17770
                     /locus_tag="KPH93_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1688120.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILDLEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDINDSTIKTGEKINLGARFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLNCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLAIMEKIFNKYSPKSIRTFFVPMMSLAITIPITLTILGPLGYNVGTIFTIIILFLYN
                     KMGFLVVGLLAVVLPFMVATGMHKALIPYAINTIGKLGYEALYMTASLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAILGVVLSSAISGTFLG
                     LFTVKGFVLVGPGLASMSMFIDPNNGNNLIFAIIGFVVAILGSFIITLIIWKEDANTA
                     ESSTEDIVEEELIEEEKEVNILVSPVEGKVIDISKVNDELFASKTLGDGVAIIPANGN
                     LYAPCDSEVVMLFETKHAIGLKTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEG
                     DLLIKFDLDKISHANLDSTVMIVNNNGSDYAYKVLNKSYGNVKKGSILFDVKGGI"
     gene            17773..19239
                     /locus_tag="KPH93_001290"
     CDS             17773..19239
                     /locus_tag="KPH93_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG1688121.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVIDMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLAAQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRIDYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            19263..20096
                     /locus_tag="KPH93_001291"
     CDS             19263..20096
                     /locus_tag="KPH93_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1688122.1"
                     /translation="MNYIIKKVLNSSVVLVHDANDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKEIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAIASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     K"
     gene            20481..21374
                     /locus_tag="KPH93_001292"
     CDS             20481..21374
                     /locus_tag="KPH93_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG1688123.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREINKADINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDVL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIKQYNLQLIDSEIQSEV"
     gene            21455..23425
                     /locus_tag="KPH93_001293"
     CDS             21455..23425
                     /locus_tag="KPH93_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688124.1"
                     /translation="MVVVTTIIGCSSGEVIKNTSPINSVKNLDFSFEVKPEIFKVSVE
                     SGGSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYLDVNIKSNLEKETSFNWPKV
                     EGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSMQFFAVNKAKYSVIYIIENE
                     FNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNKNITDVAKTYKNYIVDKGEF
                     KTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNKLKENLSDDLTNWMKELLSM
                     KSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMNTFYNEKVFESKDREIKKVL
                     DKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNTTDVINDINKSGIKNAWIGF
                     DDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEKWSTASFKDKSLYENATIEN
                     KKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEFNSWFIDCDATGEIYDDYSQ
                     NHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSSTIAFAHGIETPAFAWVDKD
                     MSKNKDSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIFIDPIYSLPLFRLVYNDSVI
                     SSHQWLWGTLKIKDEVGSRMMKEVLYNTSPMY"
     gene            23426..23698
                     /locus_tag="KPH93_001294"
     CDS             23426..23698
                     /locus_tag="KPH93_001294"
                     /inference="COORDINATES: protein motif:HMM:NF022752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688125.1"
                     /translation="MDRTEWEKHKDKIISHNKVWSAFNKKAIKKEMTDFKFISEDRLV
                     QMTEYGEDLKVLVNFSQKDIKYNDEVIKANSLVIYDADSKVIYKPE"
BASE COUNT         9088 a         2650 c         3928 g         8091 t
ORIGIN      
        1 ctaatttttt aagaaagtcc aatatatttt agagatagtt tttatactta taaattactt
       61 aagatactag ggtctttaat cttattatct tcagcaagca atagtatctt agaaataatc
      121 tcagcagttt tagggtcttc atcagcgaat ggaaggaata tcttacctct actttgagaa
      181 tgtacaggaa gtatattcaa agcacccatt ccttttttat gtacaatacc agaacccata
      241 tgcacagaat actcacctaa attacctgtt ataagagcat gagagccttc aaaattgaca
      301 ttgttaattt ttaataataa taaaagctct ttagcaattg ctatacgcat ctctacagta
      361 gaatgactag tttcagggtc aacaccacca gcatgagcga cacttacaac aaggtctata
      421 tcacgcataa cttctgagaa tagaattgga ggtatagaag ttaattctaa gcttttataa
      481 gtttttctat caaaaaattc tactgtttca atagtaggag gctctatatc tgctggtgaa
      541 aaccaatctg caagtgcaaa catctttgcc actatatttt ctttataaaa tactttctgt
      601 aatccatctt cataatcgac agtccaaagc tgatttctaa gaagtgctac agttttcttt
      661 ggctgaattt ggtgaccagc atatctgcga gatatatttt tttcagataa ttcatcactg
      721 tttattctat aatactcacg gaatacttgt ttaaatggtt gaacaatttg cttactaaat
      781 aaatcatgtt ggaactcact ccacttacca gactcaaaca aatgaactgg atgtgcaagt
      841 tttaatttat tttttgccgt caacttataa gtttcacctt ctatattgca aataaagaac
      901 ttagacttat cattataaac atatccaata ttatcatcac atacccatac tagattttta
      961 atcaaagggc agataactgg atttttatag atattggcaa tttctttaac tgtaaatact
     1021 atttcatttt tcatagcttg ctctaaactc tgttttgcac gagaatactg actctttaag
     1081 tctttttgaa cagatttaag ttcaagtaca tatggatgtt tagcaatagc ttttggtata
     1141 ctctttaaag acttattgcc tttttgaact ttagtagacg cttttccgtt ttcatcaaca
     1201 tctatccaaa ttgaagtacc atcaatatct tttggtttaa atagatgagc aacttcattt
     1261 agtttttcag tctcaagagc ccacatcata cgatttacat ctccaaatcc agcacttcta
     1321 gctagatttt ctagcgctat agtacaagct tttgattcac tttcacgacg ttgtgcacca
     1381 aattgcttgc tttctttagc aaacttttga ataaattcat aacggaaggt tgcttctttt
     1441 aggtctcctt ttttcatagg aagtaaagag aaacttctca atttatcttg atttctattt
     1501 gtttcaattt ctttcatcaa catgtcttta tcaagcttac caagtgtagc atcagcatag
     1561 agttgtgcac gtctatgaga gttaccacca ctagttatat atttagctga tttgtaaagt
     1621 atattgaatc tttcttctcc aatagtttta tatgaatcta aaaaccaatc tacatcaaat
     1681 gcaccatcaa tgaaactacg tggtgaaata ggggagaata gagcaacttc tgattctttt
     1741 tgtgcagaga atccttcatt tatatgtgcg tggaagtacc aaattgtact tttcagaccc
     1801 ttccagttta aatatttttc aattatgttt acccattgag gtgcatacat agcagattct
     1861 atcaatctta actcagtaat gtcggtattt ttaagtaaac tttttaattt ttttgaatca
     1921 tcttctttag taggatagca atttttaagt agagaactta aaacatcttt ttttgaataa
     1981 ttgctaccat atgaatatcc tctctggaaa gtttctttac caagagcgac caaaatattt
     2041 acaaaataat ttataccttc aaagtattgt atattattag ctagatgaga cacttctgtt
     2101 ggcatatcac ctctacgtga ttcaatttct ataattcggt ttgtaacagt ttttaaatta
     2161 tccttaagcc aagggtactt ttctaatgtg tcaccagccc atctaggatt tgttaatgtt
     2221 cttattatat ttgaagcgtt gtatccactt atcatttctc tattaacttg ctcttcagtc
     2281 attaatccta gttcatgtgc acgagcaaac acatttatgc tagtatagaa aggaaactta
     2341 tattctagca actgataatt tgaaaacgta gtgtagaaaa acttagtaaa agaaatatca
     2401 tcataaataa atgaatgtgc tttatcaagc caatagttta aaataggggc atttaaagca
     2461 gggcagtatt cattttgtct gtaatagttt ttagtaaatt caactctttg ttttttacat
     2521 tgttctatag ttaataagct tctaagttta tagtatattt ccaaacaata gttaaatatt
     2581 tctttatggt caaactcatt tagtaaaact cttaaaagtt ttcttgtaag tccattaaat
     2641 ttaaattttt caagttttgt atatatttta ttttgttttt tagagtcaag gacatttgaa
     2701 tatatagatg aaaaccaact tttttctgtt tcattatatc cacatgtact tatactataa
     2761 aataatatat caagtattaa tttgtaatct aagtcaagtt ttttgctagt ttgtctccaa
     2821 agttctgcca atggataatt atcaagagta tcattagaaa ctttatattt aataggagtc
     2881 aaattataag tagaataacc taaaagtact ttgtctacat tacctgacca tctttcaact
     2941 tcatactcat aatttgcatt ttcagtaata agctcatcaa atttttcata aacttttatt
     3001 attttattta tatccgtatc gagaattttt tttgcttctt tttttgttgc tacagaaaca
     3061 gaatcctcgc aagtagataa acttatgttt gaaataattt tattaagctt tgatgagcta
     3121 tgagaatcat ctttaaaatc aaattgttct ggaatttgaa ctttgttttc agggttatat
     3181 attccaaaac cattttcttt gcagtaatca ttttttgctt tacctaacaa attatcaatt
     3241 agtatttgaa cttgagatgt attcttttta gatttatcaa tatcttctat aagtaaaata
     3301 agcttattac atagttcttg tctatcagta ttgtctttta ataagtttac aatttcaaga
     3361 cctccaaggc atttttgaga atttgaagat tttatgagtg aagaagcaat attttccaaa
     3421 tttgaatcat cttgttcaag taaaattttt attactgttt gtctaataga gcttgtcttt
     3481 aaagacaata gattggttat cttatctaat tctacatctg ttagttcaag ctttctaagt
     3541 agactcaata ccgtttctct attggaaaca ctcttatcac ttaaacattc caataaaaat
     3601 tcacgttgtc tatctgtctt tggttcattt acaaaattga ctataagtga agctcttaaa
     3661 tctgaactca taagatgaca attttccata agcacatcta taaattgagc attcatatcg
     3721 tatgctgtta aagtcatcat acatttaagt atttcatgag aagaaatttc aatccaagtc
     3781 catggaaata cacttcctga aaatgatttt gtctttgaat ttaaaatttc taaagtatgc
     3841 tttaaacttt caaattgttc ttttctagca ttaaagtctt tgtcaatagg agtgtttgac
     3901 aagtcaaaat tagtttctct gttttgccaa ctatagtagt agccaacaga accttctaca
     3961 tagaaatttg taattaccca tgctagtatc tctaaatcat cacccttaat gtttttagat
     4021 gctatagaat gtttaaaata tgtactttct gtatgcgata aaaagtaaag agctacaagt
     4081 tttttatatt tttcctggca acaaagtaag ttttcaataa gagagtaaga gtcatatatg
     4141 ttgtaagttg cagtagccca taaggcaaaa taaatttcaa gattatcatt gctattaatt
     4201 gattctatac ggaacttttc atcagtcaat gatttatatg aaagttctaa acatttacgt
     4261 attattccag ttttttggtc tgaaaaacca agtcctgtcc atgtatccaa ggctcttatg
     4321 acagaactaa atcgctctaa attgttgtcc aaaatagttt ttaacatgta tattgatgca
     4381 gaaattgtac cctcatctaa actctctaca atttgctgtc taagtccttc ttgttgtttg
     4441 gctgccaaaa gaagtttaga aagcatttca tagcatttat cactatggct cttcatgata
     4501 ccaattatca tttgtcttgt aagtagagca gtttgatttt ctccaaatat tacttcttct
     4561 aaggcactta ttactttttg atttcctttg tctatttcta gtgctatttt atcactcatt
     4621 atagagtcat agtacaagac cttatttgga agagtaagat aatctataac tgaaaaatca
     4681 taataaatta aatcaacata gcttgaaata aaccttataa tacctctaaa ataatccaca
     4741 agtttatttg agcgataccc tttacgataa tatcctcctg aatatgtata atccatcttt
     4801 cttttaatta attggcaaag ttcttctact tgttcagaag taaatattaa ttctaatgca
     4861 ttttttaaag gtccagaaag gagctcgtcc atatttttaa tttcgtagta gtggcaattg
     4921 tagtatttct ttatcatatt taagaatttg tcttcaggtt ctctataata gtatgaacta
     4981 ttttctatgc agtcccaaag agattttgct aagtcgtagt actgttttga agaagattta
     5041 gtaaaattat ttttaaaaat ttcaattatt ttaggatttt tttctactct agtacgctct
     5101 aacatttatg tacctccaaa ttatttattt taaaacattc ttccagcaag caaggtaagt
     5161 cttataaaag taaatatatc tccacttttc agatttatag ggtaatcgag tttttcaata
     5221 acatcttcat ttataagcac tttgtagatt ccatcttcaa atgcctgtaa cgctacctct
     5281 attgcctttt ctacagattc tttattctca ttgtaaatag aaccaaatcc aacttttcca
     5341 atatcagctt gagattcaat ttcttcatta gttaagaagt ttattattgc tttttctggg
     5401 attttctcat tatattcaag aacattttta gatacaattt cagagatagc ttcttttagg
     5461 caattgatgt tgtcagatac atcaaaaggg gtaagttcta gcataggttt gcgtttgcca
     5521 acagccttaa ctctaaaata aatttgcata aaatcacctc gcaaaatatt aattatgtat
     5581 ttagaaattt aaaaatttat ctatttgtta tattatatca ttttgtgaat tattaatgtg
     5641 aattatggaa ttattttaca aaaaatagaa aaaaagctat cttaaagtct aaaaaagcaa
     5701 taaaagataa cttttaacta taattatata gaattataac tgtacattta ttaaatttta
     5761 attttagaat tatttttgat aactatcttt ttttatttat gaattttgtt cctaaatatt
     5821 ttttgatatc ttcttcagga gaaaagtcat caataagtat ttctttccct gtgtcttcaa
     5881 atactacaag aacttcacag tcaccttgta agaaaaattt tctaactgta tcttctttaa
     5941 cttctctaac ttttattaca ttcttttttt ctttttcaaa gtcttttgtg taataacttc
     6001 ccatcatatt aattattttt gtatatctat tcataaaata cctccatatg cgattaaatt
     6061 taaaaaatat gttataataa ttgtttgtat taacttaata attataacaa ggagtaataa
     6121 taatgactag tataaaaaat aaagatattt taaattgtat agattattcc attaaaaata
     6181 agctctttaa caaactaaat gatgtatata agtccttgcc agcaggaaat tgttctggat
     6241 gtggaaattg ttgtatggag tcagtaggta taaatttaat tgagtttttg aatatatttt
     6301 gctatcttga agatagagtt gatttaaaaa aaagctgtat aagtaaagta ttagattatt
     6361 attttgagga gtatacaaga aagaacccat gtccatttaa agatactaat aatagatgtt
     6421 taatatatga agttagacca ttaaattgta ggatgtttgg acattggaaa aaagaggatt
     6481 ataataaaaa cttagataat gttattgaga aaaataataa ctatagagag ttaatgaaaa
     6541 agcaatatgg atttgaaata aatgatgaag tagtaaatta caggatagat tattgtgata
     6601 gatttattcc aaataaagat tatttaagca aatctgagag attaagtttt tttgacgaac
     6661 ttatgacatt agactcaaat atttattcaa gtggaaatat agatatagac tttagggata
     6721 gaggtatagt tgaatatttt atagagtcat tactttatag aaatttagca tataatgtga
     6781 aaataaggat atctaaagaa tacaaaataa gaaaaagaac tatagataga ataaaaagga
     6841 ttgttcttat atagtttcat attagtaagt aataaattgt atcataaata ttgtcaaaat
     6901 gaggtgaaga aggtgaatct tacaaaagta aaaggaaata ccttttttat aaaaggtggc
     6961 acgaatacag gaatatatgt atttgaagat aacacagcac ttatgataga cccagggtta
     7021 tgtggttcaa gacctaggag agtaaataaa gtattagatg aaaataatat acaggttaga
     7081 tatattataa atactcatgg tcatgatgac cactatggag cttgtaatca atttaaagag
     7141 tattataaag atgtgtgtat aatgtcatct gaatacgata aattatatat agaaaatcca
     7201 gaattgtttt caaagtatat tgttggggga aagagcaatg tatttatgga taatatctta
     7261 aaaaataaaa tgttggatga tataaaaata gataaatcta tagaagctgg aaaaatagaa
     7321 ttaaacaatg aaatgtttga tataattgag ttccagggtc atacacctgg tagtatagct
     7381 gttttaacga aggataatgt gatttttgtt ggtgatttat tgataggaga tgaaattcta
     7441 tcaaaatacg atttcttgtt tctatatgat atacaagaac aaataaattc attagaaaaa
     7501 ttaaaaaata tagattttga gtacttagtt ttagggcatg gcaagcaagt tatatcaaaa
     7561 gaagattctt ataaattaat cgaaaaacat ttgaatgctt taaaaaaata tttatatcaa
     7621 ataagagagt tgcttgtaga accgaaatct ttagaaattt tattaaaaaa cattataaat
     7681 aacaataatt taagcaataa ttataaagag tatcattttt ttaaatcttc gttagtatct
     7741 gtcataagtt acttagttga tttagaagaa atagggtata tattggaaaa tggagaattg
     7801 ctttactata caaaaacaaa ataaattatg ataaaataaa gaaggtcata aaaaacaaat
     7861 aaagggtgag caatatggaa tacgaaaaat ggaatgagat actagcacct tatgaacatg
     7921 cagtagagga attgaaaata aaatttaaaa atataagaaa agaatattta gcaaaaggtg
     7981 aatattctcc catagagttt gttacaggca gaacaaaaaa aatatcttcc ataatatcta
     8041 agttaaaaag gataaatgca aaagatatag aaactgaaat tgatgatatt gctggtataa
     8101 gaataatgtg ccagtttgtt gaggatatat atgctatagt tgatttgata aaagtaagaa
     8161 atgatatgac tataatagga gaaaaagatt atattacaaa ttacaaagat agtggttata
     8221 gaagctatca tgttataata aaatacccta taaactccat tgcgggttca aaggaaatta
     8281 tatgtgaaat acaaataaga actcttgcaa tgaatttctg ggctacaata gaacattcat
     8341 taaaatataa atatgaccat tacatacctg agactcttgc tgaaagactt agaagagcat
     8401 ctgatgctgc ctttctttta gaccaagaaa tgagtgaaat aagagaggac ataatgaaag
     8461 ctcaggctat gtaccaaatg aagtctatag ctattagaga tactctaaat agaatacaag
     8521 agttatatga cttgggtgat acacataaag ctatgcagta tcaaagaaaa cttgatagaa
     8581 ctgaaacaga taaaaatata actgaaattt tggaattaaa acaagaaata gatatattac
     8641 tagagcaata taaggatact aaggtagatg ataaagtagt tcagtaaaaa actacttgta
     8701 gaaaatttgc taaatattaa tggggctgcc tcttaatgga aaataatttc taaaaagaga
     8761 cagcctattt agtgtaatca agaaaggaat gatattatga gtttatatgc tataggtgat
     8821 ttacattttt caacatcagt caataagcca atgaatatct ttggaagtaa ttgggatggt
     8881 catgaaaaga aaattataga taattggaaa gaagttgtta aggaagaaga tatggtctta
     8941 gtactaggag atacatcttg gggaattaat ttatcggaag ctaaaaaaga tttagatata
     9001 ataagcaaac ttccaggtca gaaaatactt ataaaaggaa atcatgatta ctggtggaca
     9061 accgttacga gtttaaataa gctatatgaa gacatgcgtt ttattcagac aaatttttat
     9121 gaatataaag attatgcaat ttgtggaggt aggggctgga tatgtcctaa tgatgttaaa
     9181 tttgatgaga ctgatgaaaa agtttataaa agagaagaac atagacttag gttatcatta
     9241 gaatctgctc gtaaaagtgg tcactcaaaa ataattgtga ttactcatta tcctcctacg
     9301 aatgataaat tagaagaatc tttatttaca aagttatttg aagaatataa tgttgaaaag
     9361 gtgatttatg gtcacttgca tggtaaagaa tcttttaaaa tgggattgaa ggggattcga
     9421 aatggagtag agtatacatt agcatcttgt gactatacag aatttaattt aataaaagtt
     9481 cacgattaaa aaacataaat tggtaaatac tataagatac atgattaaaa ataaatattt
     9541 gagggatttc ggttttgaga aaggagacac gacttatgag gtggatatta gtttttatat
     9601 ttttatatct tattccttta gtagtgatat tcagaaatta caagaatttt aagaggtctt
     9661 gtatctacag tagtatttat gtggtattag ctacaacaat aatgataagt aatatttata
     9721 tgagtggact taataaaata aaagagtcta tgtattatca caattatatc tctgatgaga
     9781 catatgatga aaagtatgta tcaaatttta aggaaaaaga aagtactgat ttaaaacagg
     9841 ataagaaaaa gaatgaagaa atatcaaata ataaaaattt aaacaacaaa caagatagaa
     9901 atatacaaaa taaagaaagt aatgaagaag gaagtaatga tataaaagaa aataataatt
     9961 ctgatagtaa tgtttcagtg aataataaat cagaaaaaca agaagataat acaaaagaaa
    10021 ataacaaata tacaagtaaa gaaaaaaata gtgaagatat aaaaaaaata gatatggaaa
    10081 gtataggaat ttttaagaaa gaaatttata atatagaaag agcagcttta gttccaatga
    10141 gagaatgtat accttacact aaggatatag caaaaaatat aactaaatta tcgagtataa
    10201 aaaaagatgt tacaaatgca agaaacaagt gtaaagaagt tgtaaaagaa tatgaaaata
    10261 tgcagattcc agaactttct aaggagaagt atacagaaac attgagtaat gctagagatg
    10321 atgtaaagaa agcatatgag cttagagaaa aatctatgga aagtgctttg aaattaataa
    10381 atactaaaag ccctaagtat atagggaaga taacagaata tcttgattta tcagataagc
    10441 agatagaaag ttttaaaaat agattgaacg atttaaaaga agaaataaac aatacagaaa
    10501 caaataatat caattaataa gatttaagga tataagttaa aagggagatt atgatgaata
    10561 aacagagtat aggaaaacta aatatgtttt ttatggggat tactcaaaga aaccttgata
    10621 atagggattt ttttattgag ttagaggtaa tttttaaatc tggaagaaaa gagtttaaag
    10681 gaattgcaat agaggaagat aataaatata aatttaattt taaaggaaaa tcagatttat
    10741 atacgtttaa tgaattgctt aagaatattt ctaaagaggc agaaaattat gatggattag
    10801 tatttaaata tgttgagcga ggaactattg ttgtaattga aggagacaat aagaaggtaa
    10861 atgtaaaata tttagataat aaggaagaag tacctaaaat agatgaattt acggcatctc
    10921 aaataaaaaa cagagattat tatgtaaaag taggacaagc aaatgcactt ctaaaagaaa
    10981 ttggagtact tacaaaagat ggtaagataa aaaatgataa aataagaaaa tacaatcaga
    11041 tagaccattt tgttgaattg atagataata ttttaaaaga aataaaggat aaagactgta
    11101 taactatctt ggattgtgct tgtggaaaat cttacttaag ctttgtctta aatttttata
    11161 taaaagaagt attaaagaaa aactgttatt ttataggcat agattattca gatgtagtta
    11221 tagaagcatc aaaaaatatg gctaaaaact tgggttataa aaatatgtca tttataaaag
    11281 aggatttgac taattataca cctaataggg atgttgacct tgtaataagt ctacatgctt
    11341 gtgatacagc tacagatatg gcaataggtc taggaattag agcaaaatct gaagctatag
    11401 tagtagtacc atgttgtcat aaggagttat tagggcaata tagatatgaa gctatggaac
    11461 caatattaaa acatggtgta tttaaagcta gatttgcaga tttaataact gatggtttaa
    11521 gaacattgtt attggagggc aatggatatg atacttctgt agtagaatac atatctccac
    11581 ttgatacacc aaaaaattta atgataagag cgataaaaac taaaacgaat aatgacaaag
    11641 cattaaaaga atataaagaa ctgaaatcac aatttggagt ggaaccaacc ttagaaaaac
    11701 ttatatatta gttaaaaatg taaaatttac gaaaagcttg cttaaatatg caagcttttt
    11761 ggtattatat aggtatagtc atatctagag ttttgattta taattaaagc tgtgctataa
    11821 tatttgatta atcaaatcaa taaatgttac atggaggtaa aaatgaacaa attgaatata
    11881 tgtattgata tagatggaac aataacaagc ccttatcatt ttcttccata cctaaatgat
    11941 atctttaata aaaatataac tgaagcagag tgtataacac ataattggga agaattatac
    12001 ggttcgggaa taagagatgt gtatgcggag tttaataata agtatataca ctcatatgat
    12061 gaagcaaaga ttgtagaggg tgctacagag gtaattagca cattatctaa aggacataat
    12121 ttatcttttg taacggctag acatgagtgt ttaactgatg taactaaaaa ttggctatcg
    12181 agacaaggat tttcagatat agatgtttac ttattaggaa gtgattataa agttgaaaag
    12241 gcaagagaac tgagttgtga catatttata gaagataacc ctttaaactc agtgcaacta
    12301 gctgatgatg gtgttaaagt aatacttctg gatactaact ataataaaga tgttaaacat
    12361 caaaatatag ttagagttag taattggata gatattgaaa agattataaa acaaggtatt
    12421 taaagatttg tagattgaaa gggggagcat atgatagatt taaaaattga aaatagaaaa
    12481 ggaaaaatcc atgtaaagag cagtgaaata aaagacattt tagaattacg tccagacttc
    12541 gaatatgtac aagatatatc aaatactata aatcaagaaa atatattggt atttgattgc
    12601 cagttgacag gaaactcttt tgatatggat gatttagata tagaagaaat attggaagag
    12661 ttaggtgaag aaatagatga gtcttatttc agtgttttat ttgaagatgt aagagcgtat
    12721 ttaaaagatg ctactgatga gatagaagca gacttacaag ataattactt atttgataat
    12781 ttgagatgtt attttgatat atacaatata aatcaagaat ttacagattt taagtttgta
    12841 tttttagttt cttttaaaga tataaaaatt tcttcattag caaacctagc taagattgtt
    12901 tctaagagac agcttatagg tgcatctaag ttttatagtt aaaatgaaat attattataa
    12961 taaaagctag tagccttgta aaaatatagg ttactagttt tttatttgtt cattttcaca
    13021 aaatttcaga aaactattta cattctaaaa aaagtaatgt atattaaaca taatgtacat
    13081 tataatggat aaaaagtttc tattaaatta ctaagtttat ttaataatca aaagaattta
    13141 tcttatatta gtatgtatta gggggtattt atgcaaagtt taaatgaaat aattgctgaa
    13201 aatttaaaaa agataaggaa agaaaaacgt cttagtttag acaagattgc acaacttagt
    13261 ggtgtaagta agagtatgct atctcaaatt gaaaagtgtg aggtaaatcc tactatatct
    13321 actttaaaga aaataactaa tggactaaaa atctcattta catcattaat ggagaggcag
    13381 gaaagtgaca tagaattgat tcaaaaaagt gatatagacc attttattga ggataatgga
    13441 aagtatataa gttatccaat atttccattt gactccaaaa gaagatttga gatatttatg
    13501 attgaaatag aagaaggtgg aaatttagat tcaaatgctg aacttccagg aacacaagag
    13561 tttataactg tttttagtgg cgaagtaacc attaaaataa atggagaaga ttacattgta
    13621 tctagtggaa attctataag atttaaagct gatgtatcac atatatatag aaactcaggc
    13681 aaaggcatag ctaaaatgag tatggtagta tattatatat aaataattta atggaactag
    13741 agtgatattt agatgatttt aggtatgaag atatattata tttaagttat aggagtgtga
    13801 tttatggatg aataataaaa aagcagtttt tttcttttgt agtgatttaa agaaagaccc
    13861 agtagccagt aatgtgataa agtgttgtga agaaataatg gatttaaatc aaactgatat
    13921 tgttattgat gataattttg tattagagtt taaggataaa aacaataact tatttcattt
    13981 tgtaaaaaca aaagatgtaa taagtcataa ttataaatat tatcttccaa ttttaaatag
    14041 acatttcaga gattatgatt ttgcaggagt tgtaaattgg catgagggag aaaatgctcc
    14101 agaacatatc ctaacagctc atacaattgg agatgttcca acaggagaat ttggaaagtc
    14161 aaatccaaga tactttaaaa atctaatcaa tgctattgag gatataaaaa atgaaaatct
    14221 acttgatgaa tttactacat taacagaagc aactcactgg tctggaacaa catatggtga
    14281 agaatctaaa ttaattacag aatattctgt acctatgttg gatattgaga taggaagtag
    14341 tagcgatagt tttaataata gtatagcaat acaggtattg gcaaaatcat taattagagt
    14401 atttgattgt gatgaacctt tgaagacttt attatgtgtt ggaggggtac attttgagaa
    14461 atctttctca gatattataa aaaataaaga atataacata tcaattggac atgtattgcc
    14521 aaatcaatgg attgtaagtg gaatgtatga tgatgaatcg ggatttgaga aactagaaaa
    14581 gtgtataaat agtattgaag gtggaattga ttgtatagtt tttcatgata aattaaaagg
    14641 tacctataaa gaacaatgtc gtaaaatagg tgaaaaactt aatgtacctg tatttaaaca
    14701 taaaatatta aaaaatccaa aagacttacc tatatggtag tcttatacag ttaatataac
    14761 aataagtcgg attaattttt aagcgaggtc aaaaatgata ttatcttatt ttgcattatt
    14821 aattagagta gtgctattag cttttgaaag agtagttgta agattattag gtgatgaaga
    14881 aggagatatt tataaaaata tagcttcatc atttctattt ttctttatag gtggagtatg
    14941 tttacttcca tttagtataa tggatagagt tagtgattgg tcgtttttaa taccatgtta
    15001 tataagtagt ttagtctatt caattggttc agtagcgtat gtaacgtcct tagcaactgg
    15061 ggaagtttca cttgttactc caataaatag tttaaattca ttgtttttac tgcttttatc
    15121 agttatattt ttaggtgaag gtttaagttt agcaaaagtt gcaggaatca taattatgat
    15181 tggtggagtg tttatattaa aaaaagtaag ctctttttta aagaatgtct ctgtaatttt
    15241 taataattta ccatgtagat taatgttttt atacatattt ttgcagtcag taggaagagt
    15301 attggacaag gctttttttg tacaagtatc tcctatatta tattcaacta tactgtattt
    15361 tttcgtaggt ttaaatttat ttatcttttt agcatttaaa aagaaacaaa aaataatttg
    15421 tgagattttt agtaataaaa aggggctttc aattttaagt ggtgctatta atggatattc
    15481 ttatttagca ttattaattg ctttaaataa tctagagctt agtatagcag agccattttc
    15541 tcaagtatct atgataataa ctttaatact tgctcatttt atattcaaag aaaatataaa
    15601 agaaaaaata ccaggttcaa ttttgatact aattggtggg tggttactgt tactttaatt
    15661 gatataaaaa atgaattata aaggtgctat tagttagtat cagaaaattt aaaaaagtat
    15721 tgacaattct tttaggcaaa tgttaatata ataatacaaa atagtataaa tagaagacat
    15781 gttactggta atgcaggcag gatcgaatat attgctttga tttttcaagg tgtttatata
    15841 ggttctgcct tttttgcatc ttgaatggcc attcataaaa aattatggag aaggagatta
    15901 caagatgaat tataaaaaaa ctgccattga tattattgaa caagttggtg gttcaaaaaa
    15961 tattttagat ttagaacact gttcaactag actacgcttt aagttgattg atagaagtaa
    16021 agtcaatcag gatgctttaa aaaaagtaca aggtgttatg agtatagtta atggggcaca
    16081 atttcaagtt gtcattggaa atagtgttgt tgatgtttat gatgaaattt taaaagtttg
    16141 tcctttagat ataaatgata gtactataaa aactggagaa aaaataaact taggagcaag
    16201 atttatagaa tttattatta atatttttca accattgatt tttgctattg caggagctgg
    16261 tattttaaaa tcactgttga tgttattagc aatgtttaat attttaaaat cagattctac
    16321 tatatataca ttattagttg ctatttcaga tgcaacattc tatttcctac ctcttatggt
    16381 agctgtaact actgccaatg tattgaactg taatcgttta gtagctattg ccgctgtagg
    16441 atacttattg cttccagcaa caacaactgc tttaagtgag ggtgtcgaac tatttggatt
    16501 tacaattcca aacattgcct ataatgcaca ggttttccca gctatcttat gtgtgtcttt
    16561 cttggcaata atggagaaaa tttttaacaa gtattcacca aaatcaattc gaactttctt
    16621 tgttcctatg atgtctttgg caattactat tccaattact ctaactattt taggtccttt
    16681 aggatataac gttggtacaa tttttacaat cattatttta ttcttatata acaaaatggg
    16741 attcttagtt gtaggattac ttgctgttgt tcttccgttt atggttgcaa caggaatgca
    16801 caaagcatta ataccttatg caattaatac aattggaaag ttgggttatg aagcattata
    16861 tatgacagct tcattagctc acaatatttc tgaaagtggt gcttgttttg cagtagcctt
    16921 acgtacaaaa gatgaaacat taaaacaaac agccttatct gcaggaattt ctgccttgat
    16981 gggaattaca gagccagctt tatatggtat tactttacaa cataaaagag caatactagg
    17041 tgttgtattg tcaagtgcga tttctggtac atttttagga ttatttacag taaaaggatt
    17101 tgtacttgtt ggaccagggc ttgccagtat gagcatgttt attgacccaa ataatggaaa
    17161 caatttaatt tttgcaatta taggttttgt tgttgcaata ttaggttcat ttattattac
    17221 actgattatc tggaaggaag atgcaaatac agcagagtca tcaactgaag atattgttga
    17281 agaagaatta atagaagaag aaaaagaagt aaatatctta gtatctccag tagaaggaaa
    17341 agtaatagat ataagtaagg ttaatgatga attatttgca agtaagactt tgggagatgg
    17401 agttgcaatt ataccagcca atggaaattt atatgcacct tgtgattcag aagttgtgat
    17461 gctatttgaa acaaaacatg caattggttt aaaaacaaaa aatggggctg agattttgat
    17521 tcatattgga ataaatactg taagtatgaa tggagatgga tttaaaacat ttgttaagac
    17581 aggcgataat gtaaaagaag gagatttgtt aattaagttt gatttagaca aaatctcaca
    17641 tgctaactta gattcaactg ttatgattgt aaataataat gggtcagatt atgcttataa
    17701 agtgttaaac aagtcttatg gcaatgtaaa aaaaggaagt atcttgtttg atgtaaaggg
    17761 aggaatataa atatgagaaa gtttccagaa ggatttcttt ggggaggagc tacagctgct
    17821 aatcaatttg aaggtggttg gaatttaggt ggtaaagggt ggtctgtatc agatgtagca
    17881 aaggcacact ttgatgtaga tgtgaaagac tataaatcta ataatgaaat cacaacaaaa
    17941 gatatagaag aaggattagc acatcctgaa gatgaagtaa attatccaaa acgtcatggt
    18001 agtgattttt atcatcacta taaagaagat attgctctaa tggcagaaat gggatttaag
    18061 acatatcgaa tgagtattgc ttggtctcgt atttttccaa atggagatga taaagaacca
    18121 aatgaagaag gtttacagtt ttatgatgat gtatttgatg agttaataag ttatggaatt
    18181 gaaccacttg ttactatgtc acattatgaa ccaccattaa atattgtttt aaattatgat
    18241 ggatggtatt cacgacaagt tatagatatg tttgttaggt atgtagaaac tatctgtgaa
    18301 agatataaaa acaaagtgaa atattggctt acatttaatg aagttgattc tatgattcgt
    18361 catccataca ctactggtgg tttggttcga gaccgtttta aggataaaaa ctttgaggaa
    18421 gtcatttttc aagctatgca tcatcaattt gtagctagtg cccttgcaac aaaaatttgc
    18481 catgagatta ttccaaattc aaaagtaggg tgtatgttaa caaaattaac atattatcca
    18541 tatacatgta gaccagaaga tgttttggca gctcaacaaa aaatgagaag tatttatgca
    18601 tatagtgata cacaggtttt tggtgagtat ccagtatatt tactgtcata ttttaaaaat
    18661 aataacattc aaatagcaaa agaagaacat gatgatgaaa ttatgaaaaa gtatccagta
    18721 gattttattt cattttctta ctatatgtct tcttgtgagg cagctgatac aactggatta
    18781 gatattacac ctgggaatac gttattagct gtaaaaaatc cttatttaga aatgagtgaa
    18841 tggggttggc aaattgattc aataggtcta cgtatatctt tgattgaatt atatgataga
    18901 tatagaaagc ctttatttat agttgaaaat ggattaggag ctaaagatat tttaactgaa
    18961 gataaaaaag ttcatgacca atatagaatt gattatttaa aagaacactt taaatgtatg
    19021 ttagatgcga ttattgaaga tggtgttgaa ctttggggat acacttcatg gggttgtatt
    19081 gatttggtaa gtgaatcaac gaaacaaatg tcaaaacgtt atggatatat ttatgtggat
    19141 gctgatgatt atggaaaggg gacatataat cgatataaga aagatagttt ttattggtat
    19201 aaaaaagtaa ttgaaaataa tagtatagat tttaaatgac caatagaagg tgagcaaata
    19261 aaatgaacta cataataaag aaagttttga actcgagcgt tgttttagtt catgatgcaa
    19321 atgataatga atttattttg ctagggaaag gaattggata tggtaaaaaa acaggagaat
    19381 atatttgtgg ttccaatgat aatcaaatgt ttgtccctgt tgaaaataca aaatcaaaac
    19441 aatttttaga attaatggat gatattcctg tcgacatttt aaagataaca caggaaataa
    19501 tagtagaggc agaaaagtta ttgaactcat cttttaataa aaacttatac ttaatattag
    19561 cagaccattt taactttgca attgaaagga tgcgtaaaga aataaaaatt acaaatcgag
    19621 tattttggga aataaaaaat tattatccaa atgagtttaa ggttggaatg gttgcaattt
    19681 atatggtaga agagagattg ggtattcaat taccagagga agaagcagcc aacattgctt
    19741 tccattttgc aaacgctata gcttcagatg gaaattcata tgatacgatt aagtatgcca
    19801 aagtaattgg agaaataatt aatatttttg ttttttcttt gaatagaacc ttagataaaa
    19861 agagtatgca ttatatgaga tttataacac atataaaatt ttttgtggaa agattttttt
    19921 cagatagtat gttaagcagt ggtgatgatt tattatttaa tcaaatgaaa aggtcaaatc
    19981 caaaagagat gttaattgca ttaaaagtta gagattttct agaaaagaaa tatggaaaaa
    20041 agttaacaaa tgaggaaata gctttcttgg ttgtccatat cgcacgagtt aagtaataga
    20101 gtaaattaca gcaatagagt gaattactaa tattatctta tacgtctaca tggtagaaaa
    20161 ttgaaagaag gtaaagtata taaagaaact gagacatatt attttgttgt ttattatgtt
    20221 tattttatga gtgtatcctg ttaaaaattt aagttaatat ttataattaa aatatgcaaa
    20281 tagttaaaat aagtttatgt ggttatgaat ttattttaac tatttttgtt taatagaaaa
    20341 ctatatatta taacaataat cgtgctttta attaaatata tttcataaat cttcataaaa
    20401 tcttcaaaat ttcgtaaaga atatgtcaca aaagtaaggt atattatatt caacagataa
    20461 tataagggag gacaatattt atgaagaata aaaaattcat tgttataata ctgataatat
    20521 caattttaat aggtgtatta gtgaaggaat attcatctag agagataaat aaggctgata
    20581 taaatgtcag taagtatata aaatatgctg atttagcaag taaaaataat gcacaagtta
    20641 actggaagta tgtagcttcc attgttgctg tgttaaataa aaataactta aaaaacgtta
    20701 aggattcaca gatacaagaa gtatcagatt tatttgttaa gaatttttct aaaaataata
    20761 aaataaataa gttaagtgat gttttggatg agttagaatt tagtaataga caaaaaagat
    20821 tggttgataa ttatatagac aatcttaagg attatggtat aaaaccagaa agattgaagt
    20881 ctgatacaaa atatatgaag tttattgatg aaattaaaac agaagcaata caaaattata
    20941 aggattataa aatacttcca tctataacta ttgcacaagc tataatagaa tctagctggg
    21001 gtaagtcaac tttggcaaaa caatataaca atctatttgg tataaaggct gacgcttatt
    21061 ggaaaggtaa gtcggtgact ttagaaacga aagaacattt agatacaatt atagatgata
    21121 aatttagaat atatgatgat aaaaatgaat ctataaaaga ccatgcaaaa tttttagcca
    21181 caaataaaag gtataaaaat aatggtgtat ttgatgcaaa aacgtatata tatcaagcta
    21241 aagcattgga aaaggcaggt tatagtactg caaaggatga aaatggaaat agtatatatg
    21301 cagctcgcct aatagaatta attaaacagt ataatcttca attaatagac agcgaaattc
    21361 aaagtgaagt gtaactttta tgtaacatag gagtgatata tttatcgagg aggtggcttt
    21421 tatgaaaaaa atattctcga tatttataag ttgtatggtt gttgtaacta ctattattgg
    21481 atgtagtagt ggtgaggtga taaaaaatac atcacctatc aatagtgtta aaaatttgga
    21541 ttttagtttt gaggtgaaac ctgaaatatt taaggtatca gttgaatctg gtggttcaaa
    21601 agaaaggaaa gtatcaaatt ttaaaaagaa taagacagag gcatcgtgga cgtatcctga
    21661 tgaaaatata gacataaata taaaaaaaga ggacagctat ttagatgtaa atattaaatc
    21721 taatttagaa aaagaaacta gttttaattg gcccaaagtt gaaggggaaa gttatatact
    21781 accactttat gagggtaagt ttataccaag taatgataag tattggaagg agtatctaaa
    21841 tgagtcaact tataatgtta tagaatcttt ttcaatgcaa ttctttgcag taaacaaggc
    21901 aaagtattct gtgatttata ttatcgaaaa tgaatttaac aatgtaattg aatttagttc
    21961 taaagacaag attaatttta catttaaaca tgaatatcca agtatcaata aaaataagga
    22021 gtatgggttt aaaatatatt tgactaataa gaatattaca gatgtagcaa aaacttataa
    22081 aaattatata gttgataaag gagaatttaa aacattaaaa gaaaaatcta aggaaaataa
    22141 aaatattgaa aaactttatg gagcaccaca tatttatttt ggagataagt ctatcatcac
    22201 aataaatgat attgaatgga ataaattaaa agaaaattta tcagatgatt tgactaattg
    22261 gatgaaagag cttttaagta tgaagtctgg caatagtgaa tttataaaag taattaatca
    22321 aataaaaact caagattata tagataaata tcaaaaaaat caaatagtac aagcatttaa
    22381 tgaagttatg cagatgaata ctttttataa tgaaaaagtt tttgaaagca aagatagaga
    22441 aataaaaaaa gtattagata aaggtatatc taaattaagt agggtagaac ttatagagtt
    22501 taataagaaa ttattgaaat caaaactgga agagagcgtg aatgatgttg ctacatgggc
    22561 aaaatataat acaactgatg taattaatga tattaataag tctggtatta aaaatgcgtg
    22621 gataggattt gatgatatgg aggaaggtta tatatcctta gagatggtta aaagagctaa
    22681 tgagttaggt tatctcgtag ggccatatga ttcataccac tctatacaca aaccagggga
    22741 agaaaagtgg agtacagcat catttaaaga taaatctcta tatgaaaatg ctaccataga
    22801 aaataaaaaa ggtgaaaaag aaactggatt tcaaggagaa ggaagaaaac taaatcctac
    22861 attgtcttta ccaagtgtaa aagagagagt acaaagtata ttggatgaag gatatgaatt
    22921 taattcttgg tttatagatt gtgatgctac aggagaaatc tatgatgatt attctcaaaa
    22981 tcatataaca acacaaacac aagatttaaa tgctagactt gaaagaatgt catatataag
    23041 agaccatgca aatatggtca taggttcaga aggtggaaat gatttttcaa gtagtactat
    23101 agcatttgca catggtattg agacaccagc atttgcttgg gtagataaag atatgagtaa
    23161 gaataaagac agtgaatatt atgttggtaa atactattct ccaacaggag gtgtaccaga
    23221 aatattttca aagcagatac ctgtaaaaga aaggtacaaa aaaatattta tagacccaat
    23281 ttactctcta cctttattta gattagttta taatgattct gtgatatctt cccatcagtg
    23341 gctatgggga actttgaaga ttaaggatga agttggaagt agaatgatga aagaagtatt
    23401 atataataca tcacctatgt attagataga tagaactgaa tgggaaaaac ataaagataa
    23461 aataatctca cacaataaag tatggtcagc gtttaataag aaagctatta aaaaagaaat
    23521 gacagacttt aaatttattt ctgaggatag actagttcaa atgactgaat atggagaaga
    23581 tttaaaggta ttagttaatt tttctcaaaa agacattaag tataatgatg aagtcataaa
    23641 agcaaattct ttggtaatat atgatgctga tagtaaagtc atatataaac cggaataata
    23701 gatttaatga aaataaagat tgtttaaatt tcaagtaaaa aataatataa tattcat
//
LOCUS       DAEMAE010000041        23606 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEMAE010000041 DAEMAE010000000
VERSION     DAEMAE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.042"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(30..299)
                     /locus_tag="KPH93_001295"
                     /pseudo
     CDS             complement(30..299)
                     /locus_tag="KPH93_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            complement(404..1207)
                     /gene="zupT"
                     /locus_tag="KPH93_001296"
     CDS             complement(404..1207)
                     /gene="zupT"
                     /locus_tag="KPH93_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG1688126.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYALLRNFLNDITLGV
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(1358..2521)
                     /locus_tag="KPH93_001297"
     CDS             complement(1358..2521)
                     /locus_tag="KPH93_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1688127.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANSGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAGAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(2581..3768)
                     /locus_tag="KPH93_001298"
     CDS             complement(2581..3768)
                     /locus_tag="KPH93_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688128.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTAEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(4131..5831)
                     /locus_tag="KPH93_001299"
     CDS             complement(4131..5831)
                     /locus_tag="KPH93_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688129.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDNGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(6074..6349)
                     /locus_tag="KPH93_001300"
     CDS             complement(6074..6349)
                     /locus_tag="KPH93_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688130.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(6468..7040)
                     /gene="mobB"
                     /locus_tag="KPH93_001301"
     CDS             complement(6468..7040)
                     /gene="mobB"
                     /locus_tag="KPH93_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG1688131.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDIDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNDILSISDFEIKNILKE"
     gene            complement(7024..8205)
                     /locus_tag="KPH93_001302"
     CDS             complement(7024..8205)
                     /locus_tag="KPH93_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG1688132.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENKNLKVIGTLYAGEVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKIKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKDETIKENQLVDVVI
                     R"
     gene            complement(8416..9150)
                     /locus_tag="KPH93_001303"
     CDS             complement(8416..9150)
                     /locus_tag="KPH93_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688133.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVANKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESNKIVKIIKGTLLE"
     gene            9512..10417
                     /locus_tag="KPH93_001304"
     CDS             9512..10417
                     /locus_tag="KPH93_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1688134.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFIKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(10577..11419)
                     /locus_tag="KPH93_001305"
     CDS             complement(10577..11419)
                     /locus_tag="KPH93_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1688135.1"
                     /translation="MTTSSKKLETISPDSKITKKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(11403..12161)
                     /locus_tag="KPH93_001306"
     CDS             complement(11403..12161)
                     /locus_tag="KPH93_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1688136.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFIGEKEVIVDDNEF"
     gene            complement(12178..12648)
                     /locus_tag="KPH93_001307"
     CDS             complement(12178..12648)
                     /locus_tag="KPH93_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688137.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(12648..13391)
                     /locus_tag="KPH93_001308"
     CDS             complement(12648..13391)
                     /locus_tag="KPH93_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG1688138.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKSNLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(13395..13805)
                     /locus_tag="KPH93_001309"
     CDS             complement(13395..13805)
                     /locus_tag="KPH93_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688139.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEVF"
     gene            complement(13831..14583)
                     /locus_tag="KPH93_001310"
     CDS             complement(13831..14583)
                     /locus_tag="KPH93_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1688140.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(14823..16217)
                     /locus_tag="KPH93_001311"
     CDS             complement(14823..16217)
                     /locus_tag="KPH93_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG1688141.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAVPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(16237..16446)
                     /locus_tag="KPH93_001312"
     CDS             complement(16237..16446)
                     /locus_tag="KPH93_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG1688142.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(16475..17920)
                     /locus_tag="KPH93_001313"
     CDS             complement(16475..17920)
                     /locus_tag="KPH93_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1688143.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQVFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            18030..18824
                     /locus_tag="KPH93_001314"
     CDS             18030..18824
                     /locus_tag="KPH93_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG1688144.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            18875..20197
                     /locus_tag="KPH93_001315"
     CDS             18875..20197
                     /locus_tag="KPH93_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG1688145.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIH"
     gene            complement(20297..21514)
                     /gene="cdeC"
                     /locus_tag="KPH93_001316"
     CDS             complement(20297..21514)
                     /gene="cdeC"
                     /locus_tag="KPH93_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG1688146.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(21697..21877)
                     /gene="ssrS"
                     /locus_tag="KPH93_001317"
     ncRNA           complement(21697..21877)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPH93_001317"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(21992..22903)
                     /locus_tag="KPH93_001318"
     CDS             complement(21992..22903)
                     /locus_tag="KPH93_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688147.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDIEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDDEDN
                     NEELNYEDEELDCEGKELDYDDELTDEGEMEDNEDELVEDKNAGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            23115..23513
                     /locus_tag="KPH93_001319"
     CDS             23115..23513
                     /locus_tag="KPH93_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688148.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
BASE COUNT         7683 a         4173 c         2564 g         9186 t
ORIGIN      
        1 tctttgtatt ctttcgacca tatatactat gcagatgtac ccataaaact accaatactt
       61 ctaaatacaa gattccagcc acttgattta tgttcatcaa aataattatt ctctgcatca
      121 tagtagtaat cagcagtata ttttattttt cttgggcttc ccttatgaaa ataaaatatt
      181 cctccaaatt tatttatcct atataaatta tatcctttag cttccatatc ttctaaccat
      241 ttctctatct tatcaggtga gctataccaa tcaaatttga attttatttt cttttccata
      301 atactcactc tccaaaatga tatttttaaa tagcttaaaa gtctatttaa ataattatat
      361 cattttgata ctaactttag gttacaatca gatatttaaa aagctattta aataatagta
      421 aactaatagc cataacaccc attcctgcaa tcaatccata aatcgataaa tgatgttcac
      481 catattctct agctgatggc aataattcat ctagtgatat aaataccata attcctccca
      541 caattgcaaa tacaactcca agagttatat catttaaaaa atttctgaga agtgcatatc
      601 caataattgc tcctaaaggc tcagacatac cagataaaaa ggaataataa aaagctttct
      661 ttttatctcc tgtggcataa taaactggta cagatacaga tataccttct ggtatattat
      721 gtattgcaat tgcaattgct atcgggatag ctattgtaac atcatcaaga gctgatataa
      781 atgtagctaa tccttcagga aaattgtgaa ttgctatagc cattgctgta aatatacctg
      841 ttctcaaaag agatttattt tttttgatat tcttttcttc tatattctcc atttttttta
      901 tttcatgtgg attttcttct tgtggaatta acttatcaat aacagcaatg atagccatac
      961 ctataaaaaa agctattacg gttgaccaac ttcccaattt ctctcccata gattttgtca
     1021 aagcatcttg tgctttagga aaaatttcta tcatagaaac ataaatcata acaccagcag
     1081 aaaatcctaa gcttatagaa agaaacttag tatttgtttt tttagcaaaa aatgctatac
     1141 aacttcctat accagtagat aaaccagcca ataatgttat caaaaatgca aatattacat
     1201 tattcaaata aatcaccttc tttttaattg atattgatta tcattttctt aatgctatca
     1261 tatatatttt atatagtcaa tatttataaa aaatatacac aataaaaaag cagagatata
     1321 aacttagtct ctgctttttc tttatagtat attaaaatta tttattcaag aaatccactg
     1381 catattgcac atataaagca gttccaattt ctagagcatc ttcgtctata ttaaagcatc
     1441 catgatgatg aggataacaa gcaccttttg actcatttct agctcccaca aatgctagtg
     1501 caccaggtgc tatgttcata aactcagcta aatcttctgc tcctgttact ttttcaaata
     1561 gtgttataca gtcttctcca aatagcttaa ttgctgcatc tgtagctatc tttgaacatt
     1621 ctttgtcatt tataactgct ggtgttaagt acccatactc taattcagca tctgctctat
     1681 atgctcctgc tgtagatttt gctatacgtt ctaaaattcc aggaatttgc tttctaagct
     1741 ctggattaaa tagtcttatt gtaccttcta agacagcttc actcgcaatt acgttaaatc
     1801 tagttccaga atttaaaact ccaacactca caactaatgg ttccagagga cttacttctc
     1861 tactaaccat agattgcaga ttcataacta ttgctgaact tgcaagcact gcatcaacac
     1921 cttgatgagg aagtgaacca tgaccacctc taccttttac agtaatttta aagaaatcag
     1981 cagaagccat tcttggccct tcttctaccg atatagtccc agattctaca tcagtccaaa
     2041 gatgtattcc aaacacacta tctacacctt ccatagcacc atcttgaatc atagctcttg
     2101 cacctttacc aacttcttct cctggttgaa aaaataattt tacagttcca ttgatactat
     2161 ctttaatatc atttaatact tttgctgccc ctagaagcat tgatgtgtga ccatcatgac
     2221 cacaagcatg cataagtcct tcatttttag atttgtattc tacatcagtg cattcaacaa
     2281 cttgtagagc atccatatca cctctaagtg ctacagtctt tcctgaatta gcccctttta
     2341 tagttgcaat tacacctgta cctccagctg atacatatgg tatccctatt ttatcaagtt
     2401 cttctttaac tctctttgat gttctaactt cttccatact tttctcagga ttttcatgaa
     2461 attcccttct aagcttaatt acatagtcct tataactctt tgttatttct ttaatatcca
     2521 ttcccgacct ccaagtagta cacattcttc ttatttaaaa tactttttat cacttaaata
     2581 ctatattaga tttacaaaga tacctgctat tactacagaa gctattgtaa ctgtagtaaa
     2641 tcctccaaca agcatctttg gaagcatttt atccattaaa aattcttttt cttctggtgt
     2701 ttcagccaaa gcctttactg cctcttcagt taatatatag tttggtggaa aaccataaag
     2761 tgatgtaagc gaaactgaaa aagccatttc tttactttca cctaaaagct tacctactat
     2821 aatagagaat attcccatac ctattacacc aaatattatg attcctacaa gaggaactgc
     2881 catttcaaca agcattgctg gcgtagcttt tgctaaccct gcaaatataa aagccatcaa
     2941 tgatgtcatt aaccatccaa atgaaccaga tttattaagt ggttttctat caactattcc
     3001 aagttctgat gctattacac caaatattaa acatataaca tatcttgata caacctcatt
     3061 tgtaagattt gtaaattgta ctgctaacca tgctacaaat cctaatctaa gtattatcat
     3121 atatgtcgta tcatattttt ctggtaagtc tggaattatc ttaaatctgc ttttttgttc
     3181 aggtaaatcc ttaacttttt cttcagctgt atttacagtt acttttccac ttctaaagtc
     3241 tgataaaagt ctttttcctt cttttttaag cattaatgct gtaataggat aacctgcaaa
     3301 tccttgtgca acatacatca aaattgcaag tactgacaaa cttgcaagac cttttgctgt
     3361 agcagcatct gccatcatta tagatgcaac tagtcctcca gtaagaggag gtgtagctat
     3421 tactgctgta tcccatccaa atactactgt ccctaatgcc aaagcaccta cacatattcc
     3481 tactattcct gccaaggcta ttactattgt tttccattga cctgctaatt ccttaatact
     3541 catcatagtt cccatatgag ttattaacaa aagcatacct aattgagcta atggtgttcc
     3601 taaagcagct aaatcaacaa tattttctgg gaaaaaagtc caaaatccta ctataaatat
     3661 aattgctgat acaaaaaccg atggaacaaa agctttagtt tttgctgaaa ctatatctcc
     3721 tatcgtcagc gatattaaaa ctagacaaaa agctgttatt gcctccatac aaaatccccc
     3781 ctcataataa atgtattttt ataaaatgta tgtatagtta tccattattt tactactttt
     3841 gaaaaacatt tcaagtcaaa tttcgatttt tttcataaaa ttcgataaat tccataaatt
     3901 aaagttagtt ttctaaatta ttctttttgt aaaaacgtta tcctaagtat tggctatttt
     3961 actctattct tataaataga attaaatgta atttgcaaaa aataaaacat aggatatatt
     4021 ttattcataa aacacatcat caatataaaa tgaattgtct ttatgaatct taatatatcc
     4081 tatgtttgta agtttaatgt gtatgtttgc tatggtgtaa aatttatata ctagttattg
     4141 tgtaacctac tatatactcc atttttgttt actaactctt catgagttcc tctttcttca
     4201 attccgttat ccgtcaatac tatgatttcg tcagcatttt ttatagtagt caatctatgt
     4261 gcaacaacta aatttgttct atctttactt aattcttcta aagactcttg aatttctctt
     4321 tctgttacat tatctaaggc agatgtagcc tcatccaata ttattattgg aggattcttt
     4381 aaaaagattc ttgaaattga aattctttgt ttttgtccac ctgataattt tacgcctctt
     4441 tctcctatat aagtatcata tccatctggc aaagtctcta caaagtcatg tattctagct
     4501 tttttagcag cagctatcat ttcttcatca gtagcattag ggttcccaca aagaatatta
     4561 tctcttatag ttcctgtaaa taaaaataca tcttgttgaa ctatacctat attttttctc
     4621 aatgatttta agctaacatc ttttatactc tttccatcaa tttttatatc acccttatca
     4681 aattcataaa accttggcaa tagattacat aatgtagtct ttcctcctcc agaaggtcca
     4741 actaatgcta tagtcttacc agcttttata gataaactta aattatccaa aacttgagtc
     4801 ttatcttcat atgtgaaaga tatattttct atctcaatgt caccttttac attttcaagt
     4861 tcaataggat ttttagcttc tttttgatgg tcttgttcca ttatctctat gaatctttta
     4921 aaacctgtca taccattttg atattgctct gtaaagttta ttaatttttt cataggttgt
     4981 ataaacattt ttacatatag tatataagca gcaaaatcac ccaaagtaat ctttcctata
     5041 taagtaaaat aaccaccagc tattacaact acccactcta agatatccat gtataaattc
     5101 ataccagagt aatattctgc cattacctta taagaatctt gtcttgctct tctaaataat
     5161 ttattgcttc ttacaaactt atttaactct tctttctcag tacaaaaaga cttagttacc
     5221 ttcatacctg ctatactatt ttctaaagta gcatttacat cacctatagt aactctactc
     5281 ttcataaaag caatattcat cttatctttt ttagctattg caaaccaaat tataaacggt
     5341 aatatagcaa atattataag cgtaagagga atatttatat ctataagtat aaagaatgaa
     5401 ccaaccaaca ttactataga aatgaataaa tcttcaggcc catgatgtgc aagttctgtt
     5461 atatccatta aatcatttac tattctagac atagttactc cagatttgtt attatcaaaa
     5521 tacgtatttg gaagtctttg taagtgtgta aatacctcat ttctcatatc agcttgcata
     5581 tctacaccaa tgacatgtcc ccaatactgc ataaaatagt taagccctgc ttttgctata
     5641 aatataagta taagtgctac tgcaaaaact actaacattc ttaagttttt atttggaaca
     5701 acgtcatcca ttatatttct agttatcata ggataaacta aatcacataa ggaaaataga
     5761 aaagctgcta taaggtctag tgtaaaaatt ttcttatatg gcttataata tttgacaaat
     5821 tgttttatca taagtattaa atccctcctt tattagtact tctaaaaaat acccataaat
     5881 tagtataaca taacaataac tttaaatctt ttataaactt acaaagaaat aacaatcttt
     5941 ttcataaaat tgaagattta tatcatatag ttgtatactt gaaacaacat aaaaagagct
     6001 atatctctat atttaaaatt ttataaagat acaactctat atttaatact aaattttttt
     6061 tataaataca aaattacttc aatgcattca atatcttttt atctaatgct gttgtattta
     6121 tacctgttat atatcctgtt gcctttattg taggtatatc ataagttgtt ggtagtatag
     6181 tagtagatac gattaaatca gcaccttctt gttttgttga acattcagca attttacatt
     6241 gttcaacttc tgcttgtata ttattttctt taaccaatct ttctactctg tcacaaacta
     6301 ttgttgatgt cgcgattcct gctccacatg ctactaatat ttttttcata tattgtcctc
     6361 ctacaatttc tttaatattt tataactcaa aataaaattg ataaatttct tatatcttta
     6421 tttatataaa taattatatc attttatata aaaagagcat cttaaaatta ttcttttagt
     6481 atgtttttta tttcaaaatc acttatactt aaaatatcat tttttataca agactctatg
     6541 tactttgata tatttttata atcatttaaa ttaaactgtt caatattcaa tttatattct
     6601 atatcactga caatacctat caaatcagac ttattagata aaatatcttc atacttacct
     6661 tttctagtta cttccaatct tttaaatcta taatttttat atccttctat gataatcaag
     6721 tccatatctt catataattg aatcatttta tacaatggat tcttctcttt taattcctca
     6781 atatatgcta ctcgtttatc tgatattata catacttttc tcgccccaga ttttctatgt
     6841 ttataagtat cttttccttc ctcatctata tcaaaatcat gacaggtatg tttaataact
     6901 ccaacacgat accctttttt atttaattct tttattatgc cttcaatcaa cgtagtttta
     6961 cctactcctg aatatgagac aactgacaat atattataca taaaattatg tcctttcata
     7021 aaactatcta ataacaacat ccactaattg attttctttt attgtctcat cctttttaat
     7081 cttaatcata caattacttc ttagagttgc agataatcta gcttttgcat ctttcttatc
     7141 atttagctct acatacaatt taccttcact cgtatatagt tttccactta caaatctatc
     7201 tttatttgaa agtttattaa attctccttt aaatatagct tttatatgat gtattccctt
     7261 cttatatgtt aaatactcaa taacaggaac tatcacatta tcaaatacca ctattgctgc
     7321 atgtggatta ccagaaagac caaataatag cttttctccc aatgtaccta cataacaagc
     7381 tccaccaggt ttaatgttta tcttccaaaa taattctgtt gcccctattt ttttgaatac
     7441 atctggaatt aaatctttat ctccaacaga aacaccacct gtagtaatta aaaaatctac
     7501 actctgtaac atattcttta tctttttttc taactccaat atatcatcag agcaattgca
     7561 tattgtatct gcttgtattc caagttttaa taatcttgaa tataaggtat atctattact
     7621 atcgtatatc ttaccattct ctaaagtctc atttatatct tgtagttctg tacctgtatt
     7681 caaaattcca acttttaaat catcataaac agttactttg gatattccaa gacttgctaa
     7741 tactccaata ccatcatatc ctatattctg atttttatta ataatcaatt gccccttttt
     7801 tatatcctca cccttaaata tgtaattatc gtattccttt aattttacat ttaaagttat
     7861 catatcacct ataaccgtta catcttcttt ttttattaca caatttgcac cctgtggtat
     7921 ctttccacca gtcattatct tcactgcttc atttttattt aataccctat taaatacttc
     7981 tcctgcatat aaagtcccta taacctttaa atttttattt tcagaatttg cattatatgc
     8041 ataaccatca tagggtgacc tatcaaatgg aggactatca attgttgaat atatatcttc
     8101 tgcaactact ttatttactg catctataag atttactatc ttgaccctgt ttattggtaa
     8161 aacatttttt tcaataattt ttatggcatc ttctaattct atcattttaa atactcccca
     8221 atatattttt tatttaagat atttctatgt aatatattat agattataaa agttggaaat
     8281 acttttatct tatatttaat tataactaat agttatctat aagtgcagtt taaattttat
     8341 aaatctaata aaaaaactgt tcccagctag aaatttcctt ctatcttgaa acagcatcat
     8401 atgctttaga atattttatt ctaataaagt tccttttatt atttttacaa ttttattact
     8461 ttcaatagtt tttatagatt ttattttgtt atccacaata cttattgtaa gtacctcttt
     8521 ttttaatatt ttgttatctt ctttcaatct atagctctta actactttac taccttcatg
     8581 attatatgct gttaaatcat aattcgcatc tcctacaatt tcatttattc tagaatcttc
     8641 aacaccaaca tactctccca agtcaatatg tctaaaattt tctagtgata cagccgattt
     8701 ttcatggtca cttttttcaa tcacaatgct agattcagca tcaatgactg atataccttg
     8761 tgatgagaaa gaattggttt caacattgac aactttattt tcgttaatat caacatataa
     8821 agcacttcca tctagttcat catttccagt atattttgga taaactaatg tcatataact
     8881 tgatgtattt atatcttgca ttgtatttga ttcttttaaa actttaggtt ttatataata
     8941 aaatgtatta taaggtatac ccatattctt tataatgtac ttataatctt tattcataat
     9001 ttcattaaca tcattttgaa cttttgtcat agttttttgc tgtgttgctt tatttgctac
     9061 ttgatttttc tctttagtat tttctttatg acatccaaca agtataatac ctaaaatagt
     9121 tacacaaact aatgaccttt ttaacttcat aaatcaatcc tcctttctta aattttatag
     9181 tatttatcta tatctcaatt tacagttatc tcttttttca ataattatat ttattatatt
     9241 ataaaataat tatataatat ttatttgaat ttaataagat taagaagcta attgtaattt
     9301 ttctgtaata aaaaacactt taaagttgaa ttttgaaaat tcaatttacg atatttttat
     9361 tgtaagtacc ttacaacatt ataccaaatt attcctaaac aatcaaaaac tccatgaata
     9421 tttaaaggtt ttctatttta aaaatcacct ttgactatta atttatcaaa tatgttataa
     9481 tagttttaga taatttaagg aggattaaat aatggatttt aaacaacttg aagtatttgt
     9541 tgctgttgca aaacatcaga gtttttcaaa ggccgcaaga gaactttttt taactcaacc
     9601 tactgtaagt gcacacatac agaatttaga gagagagttg gaaactgtcc ttataaatag
     9661 aagcaacaaa gttattacac tcacaaaatc tggtgaaatt ctttatgaac atgctatata
     9721 tatattgaat aattgcaaac gtgcaattta tgatataaaa gaatattcag gaaaaataga
     9781 aggtattata gatatagcat gtagttccat acctgaaact tatattttac ctgattttat
     9841 aaagagtttt tctatgagtt atccagatgt aaaattttcc ataagccatt atgattctca
     9901 atatgctata tcagaaattt taaatgaacg aataagcttt ggattagttg gttctaaaat
     9961 caataatcct caaatagagt acctagactt attagatgat gaacttgttc ttatcactcc
    10021 ttcagatttt aaaattgata ataaaaataa ttgtatcgat ataggagaat tggcttatct
    10081 aaattttatt atgagaaaag aagggtcagg tacaagaaac ttaatactaa atacacttag
    10141 taaaaataac tttccagtaa gtaaattaaa tgtaatagct catgtagaat caaatgaggc
    10201 tattaaggaa atggttagat taggtcttgg agtatccttt atatcgtaca tttcagctat
    10261 tgattatctc aatgcaggta aaataaaatg ctataaaata aaggatgttg attttactag
    10321 aaaatttttc tttatttact ctaaaaagaa aactttttcc ccattagaag ataaatttct
    10381 aaacagatta tgtgagtatt tcgaaattat aatataataa ctttaataaa gtagtactta
    10441 attttaggtt tataactaat taataagcta attataacta tcacataaat gcttataaat
    10501 tattatgaac cgtatttctt ttttatttaa tttagtcaaa aaatccctcg ttcaaatgaa
    10561 cttgggattt ttttatttac actactccaa tagcagctaa tactatacaa ataatcataa
    10621 ctgaaattaa tatcgtagtt gtctttacct tttttcctaa taaccaatac ataataccga
    10681 atatcatagc tggaagcata catggcatta tttggtctaa gtaagtttga ataggctctg
    10741 cccattctcc actacctaca ttaacactaa gttttaacat tacattagat gcagtcatac
    10801 ctccaattac cataagccct aacattgaag ctgcttccat aacttttgaa actatacttg
    10861 aaccactcac atctttaaag aattgagttc catacttaaa tcctttatct agacatatat
    10921 agcgtataat aaaatgagga acatttatta ttaaaaggaa taaaatcggc cccattatgt
    10981 taccctgaga agctaaagct ataccaactc cagctgctat aagctttaat gttccccaga
    11041 aaaatgaatc tcctatccca gaaactggac ccattaaact tgatttaaca gcatttatac
    11101 tattagcatc aaattcttta ttctttgcat tttcttcttc catagctcca gatataccta
    11161 ccaaaagagt acttatatga ggagtaactg acataaattc taaatgtctt tttaaagcct
    11221 tagatttctt ttctttatca tctttataca atctacggat tattggtgcc atcatgtatg
    11281 catatgctat attttgcata cgttcgtagt tccaagatga atctaatgtc aatgaccttc
    11341 taaaacattt ccaaaagtct tttttcgtta ttttactatc tggtgaaatt gtctctaact
    11401 tcttagaact cgttgtcatc tactattacc tccttctctc ctataaagtt aacatatatt
    11461 aaagctattg ctaatccaaa caatgcaact cctgttactg ttatgtttag gtaagcaacc
    11521 aatataaatc ctaataataa aaatgctact gttttcttat taagcatcat tctagctaac
    11581 atggcaaaac ctattgctgg tattacgcct gctgcaacgt ccattccatg agttacatat
    11641 gatggtataa atgaaagtat tttttctatt actggaacac ctaatgaaaa tcctatacct
    11701 actattattg ctaatattaa tttatttcct ataccaaata ataattgcac acgttctacg
    11761 ccttcaatat tcccctcttc tgcataacgg tcagcaatat gagctcccca tgtaagtact
    11821 actgtcatta agaagttatc tataagcaat actagagttg ctataggaac tgcaagtaca
    11881 actgctgctg atacatcttg acccgttaat attacaaacg ctgtggcaat aatagaacca
    11941 gacacaaaat caggaggatt acttgcccca acaggaaatg cacccataaa tactaattct
    12001 aatgttgaac ctattactat acctgtatga agattaccta gtgctaaacc aacaagtggt
    12061 gcaacaacta taggtctatt atgcattgta gaacctacaa aactatgcat atatgcaaag
    12121 aatgcaatta gagttatcaa aataacttgt gtaaattcca ttttcatccc cctaaaatta
    12181 tattaatttc attacatttg tagcatcatc atcaggaact aattgtacaa atacatttac
    12241 gccttctgaa tctaactctt taatcatctt tatatcatct tttgatacat gcactgcctt
    12301 agatatattt tcacgattat catctgattt tgttccacca agatttatag atttaatagc
    12361 ttttacctct ttagcaagtc tgtaaacatc ttctacagat tcacaaagta tcaataagtt
    12421 gtatttatca gtaacacctg agttaagtgc ttttatagaa tctgctatgc ttttcattac
    12481 taacttaaca ttagatggtt tagccatttt aagtcctgcc attctcaatt catcttttaa
    12541 taagtcatca cttgctatca atatacagtc tgtgcttaaa aactttgtcc atgtaaatgc
    12601 tacttgtcca tgtataagtc tgtggtctac tcttactaat ttaatcatta taattcctcc
    12661 ctaatttttt tgatttcatc catgaatgtt tttacgtgtt ctacactaac atccccaaaa
    12721 tctttgtaat tatctttaaa gtaacttcct attattgcca tatctcctaa tttaagctgt
    12781 ttttttgcat tttctaatgt aactccagca gctataacca atgggaagtc tcctaaattt
    12841 ttacgaaaca gttctatttt ttccattgat gtttcttgac ctgtagcatt ttctgtaact
    12901 gctatagcat cacatcttgt cattcctatt tttaaatctt cttcaacatc attttctgat
    12961 aatactggct gatatttaaa acgtacgcct ccaattaaat acgctgggca ttttgaacgt
    13021 tgtaatttaa aaaattcttc taaagtagcc tcgtctctag gtttaacatg acctactaca
    13081 gaatcaactt gtatataact agcattatat tttgttgcca gttcaaatcc catagtatca
    13141 acatttaaac agtttactcc atatggtatt gacaaattag attttgacac atattcaagt
    13201 attctttcta aatcataata attaccatag taattttcta acattattcc atctacacca
    13261 ttttcttcaa agattgtaat ctctttttta gctctttcaa agatatcttc tggagtatct
    13321 ccttttaagt gtatcattgc tataattggt tttttacttt taaatactga taatattccc
    13381 ttttttttca taaactaaaa tacctcttct tctttaattt gtaatgtcac gctttgtatt
    13441 cctcttctag attcttccac taattggtct atatcttcaa ctgttaattc atcagaaaac
    13501 cttgtaagaa gttctaatac catattcaaa ttcattccag aaaataactt aacattatgt
    13561 agattagtta atcttaacat tgctgtacat acacttgctc catataagtc ggataaaatt
    13621 atatattcac attctttatc ttctgatatt gttttttcta attctaaagc aaaatctgaa
    13681 gcactttgtc cagggtacaa accatacgaa ctaacacaat ctgctaaatc tcctactatc
    13741 atttttactg catttaaaag cccttttgaa aagtctccat gagatgctaa tattatcttt
    13801 ttcatactca tcctcctact aaattataga ttatatttct gctgatactg catatttgta
    13861 cttatcactt cttataaatt gctcattgta atataagaca tcattatttt ctgtatacac
    13921 cgattgtctt actttcaata gtggcatatt acttgcacat ttaagatggt cagaatattc
    13981 tttgtttgaa tgttttactt caaatatatt ttctactcgt ttaggtaatg tgcttatatt
    14041 attagatata acctccatta aagatttatc ttctagttct tcttttaata aaaacatgta
    14101 atctattcta aaataatcaa cttcaaaaat aacaggtact tcatctatta atctaagtct
    14161 tttaatacat attaccttct cttctagttc aatacttaaa gcttcagcta cctgcttatc
    14221 tgcttttttt attaaaacag agattatttt tgtacttggt tttgcattaa tacgatgaca
    14281 agaattagta aaactatttc ctgcacatgt tgcttctatt ctctctggta ttgttacata
    14341 agtcccttta ccacgttttt tacataatat atcttctttt acaagttcat taagtgcatt
    14401 tctcacagtt atacgactta tattaaattc tttacacaag tctccttcag atggaagttt
    14461 atctcctggt ttatatatgc cagatgctat ttgttttttt atgttgtcat atagttgttg
    14521 atataaaggg acaaaggaat ttacttctaa gtccatttct tttttttgtc tcttttcatc
    14581 cattttgctc acctccctat gtaacatttt tataacatta tataacaagt tctttattac
    14641 atattataac aagatgaaat cgttgtcaat ttttttttaa ataattttta atttgacagt
    14701 attttctttt attttctcta gtgtgacaaa ttgctatttt tacataaaaa taagactgtt
    14761 gtacaacaaa ttaaattgtt tatacgacag cctttattca cttattacac ttatattttt
    14821 acttataaat ttatattaaa aaatgcctta tatcccttat acatttcata tatgtctact
    14881 ttagagacaa tctcatgtgg tgcatgcata tttaaaactc caacaccaca atctataacc
    14941 tctgcacctt gatttgccaa tatatgggct atagttccgc ctcctccttg gtctactttt
    15001 ccaagttcag cagtttgcca tactacattt cctttattaa aaattctctt tacttctgac
    15061 ataaattcag cattagcatc attacatcca ctctttcctc ttgaacctgt atacttagtt
    15121 aaaactactc catttcccat atatgctgaa tttctctttt catatgcttc tccaaaattt
    15181 gggtcatatc cagcagaaac atcagctgat aataccttac tatttgctaa agctcttctt
    15241 actttaatat cacaataatc tccttctaaa gcaatcagtt cagccacagt attttcaaaa
    15301 aactttgagt gcattcctgt attcccttta gaaccaattt cttctttatc tacacataat
    15361 gcaactgaag taaattctgg ttgttcagtt tctagtatag cttttacagc agcatatgaa
    15421 cataccctat catcatgccc atatcccaac accatagacc tatcaagccc taaatctcta
    15481 gccttaccag ctggaaccac ttcaatttca gcacttaata aatcttcttc aactatattg
    15541 tatttctcat ttaaaatctt taatatattt gctgttatag gttctttttc ttcatcctct
    15601 aaaggaatat tcccaattaa tatatttaaa gcttctccac taattccttc tgatagtttc
    15661 ttttgcattt ggtctccagc taaatgaatc aataaatcag ttacacaaaa tactgggtca
    15721 ctatcttctt ccccaataga tatgtccact tttgttccat catttaatat aactatgcca
    15781 tgtaaagcaa gtggaacagc cgtccattgg tattttttta ttccaccata ataatgagtc
    15841 ttaaaaaaac ctagattact ctcttgatac agtggatttg gttttaaatc taatcttggc
    15901 gaatctatat ggcttcctat aatcttcata cctttttcta tataattttt acctattaca
    15961 aaaagtgcta ctgccttctc tttatttata acataaatct tatcaccaca acttatgctt
    16021 cctttttcca tagcttcatc tattgaaata tagttatttt ctttagccaa ttttataatt
    16081 tcttttactg aaagtctttc agtctttgac ttacttaaaa actccatata attgcgagaa
    16141 tattgcataa tttgttcaat agtattttct tttctttcaa aatcccaagc attcttaaat
    16201 tcgtacttta agttcatatt ttcctcctaa taagttctaa tttttattgt ttttcatgta
    16261 ttgctcatat tcttctggtg aaactacttt gacactatct agtcttgatc taaagtgggc
    16321 tctaaagttc tctaaatatt ctttccttaa aatttctctt tctttgattt catcagcaga
    16381 aaggattccc tctttatttt tctttgctaa ttcattaatc ctatctaatt tcttcttgtc
    16441 aaacattttt tcctcctaaa aaacttctat ttatttaata aaataattta ttgcttttta
    16501 tatctaactt gtttttatat tcacaaaaaa actcatctat attatcactt attatagata
    16561 ttcccttttc tagttcttcc atgcttgggc gagctatatt tatcctaaag aatctatcat
    16621 ccactagatt gtcaaaaaaa taagttcctg gaagcattga cactcctttg tttaacataa
    16681 aatttgtaaa tacttgagat gtatatcctc ttggtagttc taaaaaaaaa tttattcctc
    16741 cacaagattt tctaactttt aatttaccac tcaatttgtc attaatcaag ttttttgcca
    16801 atttatactt atcagtatat atcttctcta tatgactcaa ataattcttc caatcaaaat
    16861 tattcatata gtaatacatt gatttttgaa ttaaacctgg tgttgatata tctgatgaat
    16921 atttagccca taaaactttt tttaacaact catttggcat ttctacaatt cctatcctca
    16981 gacctggcat aagtattttt gaaaagcttt ttatatatac tactctattt ttatcatcat
    17041 aacttctaat tgttctattg tcctttgatt caaaaacaaa atcacttata aaatcatctt
    17101 ctattatata aaaatcatgt tcttctgcca attctatcaa tttctttttt ttatatgttg
    17161 agtaagatat tccagttgga ttttgaaaat taggcataat gtatataagt cttggtttta
    17221 ttttttccaa ctttaatttt aaaataccta tatcaattcc atcatctaac ataggtatag
    17281 atattatttt tgcacctcta cttttaaaca cttcaattgc tccattgtaa gaaggttctt
    17341 ccataaaaac aacatctgaa taacttataa gtcctttaca aactatatct ataccttgtt
    17401 gagcacctga tattatttga atattttcct tagtagtttt tatatttcca tcacttagat
    17461 actctaccat tttttctctt aattcttcag agccaagacc ttcatcatat tcaaatattg
    17521 aactaccatc tttagataat gccatattta cagcattttt aaatgaatcg ataggaaaca
    17581 tatcattaga tggattccca ttgtcaaaat gaattaaatc ttctctttgc acaataatct
    17641 tatttttcct gttatctata ccacttttat aagaatttaa acttgataca aaagtaccac
    17701 tccctataat tttatataca tatccttcat tttctaaaag ctcgtatacc ttaactatag
    17761 tagtgttatt tacttgtaaa aaatttgata gttttcttat tggaggcaat ttttcatgtt
    17821 ctactaactc tttatttatt atcaattctt ttatatgatt aaatatctgt atatatttag
    17881 ttgatttttt ctcatttaaa tctattctgt atttttccaa atttatcacc gctcattgta
    17941 tttatatttt attgatgcat ttatgtatat ttgatattat ttagtatgta tatataatac
    18001 caaaattttt ataaaaaggg gaaatattta tgttttacga ctatcacatg cactcaagtt
    18061 tctctacaga cggaaaatct acaatggaag aaatggtaaa aaaatctatt gaactcggtt
    18121 tggaggaaat ttgtttcaca gaccatgttg actatgatgt atatgctgat gattcttttt
    18181 caattgtcta cgaagattat tttaagagtt tagagactct tcaaagcaaa tataaagata
    18241 aaatatcaat aaaaaaagga attgaatttg gagttcaaac tcaattaatg gatacataca
    18301 aaaaggaagc acatcaatat ccattggact ttataatatg ttctatccat gctatagata
    18361 caatggactt ataccttggt aattatttta aagataaaac tcaacatgaa gtttatgaaa
    18421 attactacct ttatttatat aatattgtta agaattataa agattactct gtcttaggtc
    18481 atttagattt gattaaaagg tatgcacctt atgacactat tttagatgat agactatttt
    18541 ctgatataat tgaagaaact cttaaacaag ctatttatga tggcaaaggt atcgagataa
    18601 atacctcttg ttatagatat aatcttccag acctaacccc atctaagtat atacttcaaa
    18661 tgtacaaaga cttgggaggg gaaatcataa ccacaggttc agattcacat catataagtc
    18721 aagttgcatg tgagtttgac tacatatatt cattactaaa aaatatggga tttaaatatg
    18781 tatctaaatt taataaattg aaaccagaat ttataaaatt ataaattttt acacctcctt
    18841 gtgaatatat ttaaaagata aggaggtgat ttttttgaaa gtaccttatt tagtactatc
    18901 aacagttatt ttatttatat ccaattttat tgtaagaata tttggttttt tatataaaat
    18961 attcttatca agagcactag gtgaaacagg tttaggtatc tatcatatga tttttaactt
    19021 tttgatgatt tgcttagctg ttacaacaac aggtatacct actgcactta gttgtctagt
    19081 tgcaaagaga aaagctttaa acgatagaca caatactaat gctttattta tatcaacttt
    19141 atatatatca tttttcgtag cactaattat atctatagtt gcatctttca atagttcttt
    19201 tttatcactt aaatttttaa aagatgctaa attaaatcta tttatactag ctgtttgtcc
    19261 tgctatagtt ataataactc tttcaaacgt actaagagga tattactatg gcataaagaa
    19321 tgtaaaaatc cctgctattg gacaaatcat tgaacaaatt ggtaaaatat tatttgtttt
    19381 tttacttgtt atgtacataa ataataaatc tatgaattgt tatatagctc tattaggtat
    19441 atcgattggt gagttgagta atattatatt tatgcttatt tgcttatggc gagattcatc
    19501 atttgacaat aggtatatta taagcataaa agatttttat aattcttcta tggaaacatt
    19561 aaaaatgtct attcccataa cttgtaatag aatgtcaaac atacttttgc aatctataag
    19621 ttctatgatg ataccatcaa gattagcatt atcaggaatg acttatcaac aatctttaag
    19681 tatgtatggt attgtcagtg ggatggtaat gccttttata tttttacctt ttactgttgg
    19741 ttcagcgctt attgtaaatt taataccaac tatttcacag gaaatggctc taagaaagcg
    19801 caaaagtgta attaaaaaaa tcaaatactc catacttctt actttatttg taggcatact
    19861 atcatcaata tttttttatt tctttggaaa agatttatgt atattagtat ttaaaaatac
    19921 acttgcagga gaatatctaa aagctatgtt tttagttcca ctatttatgt ccctaaatca
    19981 aactctatct ggcattttac attcaataag aaaagaatta gcttcaagta taaatactat
    20041 tactggtatg ttaattcaac taatagcttt atatgtattt cttccaattc caggacttaa
    20101 tatttatgca tatatataca caatgacgat agtatcaatc tttacttgtc tattacatac
    20161 tatagtgctt tttaaatcac taaaatctat acactagtta taaaaacgtg aaggctacca
    20221 atgtaaatta tacactttgg tagccttcct tagatgattt ttatttataa tttaaactgt
    20281 ctatatatct acctatctat ctgtggcaac ttggctttcc acttccatga ttaggtccac
    20341 attgcgaaaa atcacatact ttagagaaag tatcaacaag tcttggttga attggatttg
    20401 gtgatgctaa aactactatt tgttcactta ttaatgtgct aactgtttct tgtaagcaaa
    20461 gtactaaatc tatatcattt ggtataatta attcatagaa aggttcacaa cattcgttgc
    20521 attctaattt taatatagat tttcctatac aatctacagt taagttagtt tgatattctt
    20581 gtcttaaagt aacagctctt ccgtcagctg gcaagcatat tggtgctgaa agtgtgttaa
    20641 aagtaaattc tacataatca actcttctct tacatccacc tcctgctcta actgctggga
    20701 aagctaatgc aacgaactct gtacatccac atctacctct tagctctagt actaaattac
    20761 gaactgctac agttaatcct ctttgtttat aagctactga tttgcctttt ccttgtctac
    20821 agcactcagc gtttctttct ccacaaactg caaattcaaa agttgtttca cagtttcttc
    20881 ctactatatc tcctaatggg tctaaatcga aatcttctaa agttgtcatt cctgtgtcta
    20941 taactattcc atcattactt aagcatattt tttctatagt tacacttgct tgacctgcac
    21001 ttggaactga accaaatact tctactactt ctgtttcaaa agttaatggc tctccatttg
    21061 gtcctgttgc atctgtaaat gtttggaatt gcatagtatc aaatattcta ttagcctcta
    21121 ttgcaaatgg cactgcattt gatacagaga agtttcttgg agatatagga ttacagcatt
    21181 ctggtccaca tttgttttca aaacattcat ctgaatccat ttcacatggt tcacaatcac
    21241 atttacaatt gtcatggcat ccacaattgt catcacatgg attaggtttg catggattgc
    21301 atgggttagg tttacatgga ttacatgggt tgcatggatt gcaatcatca tggcactcac
    21361 acttattatc agtattacat ccattatgat ggtgtgattt tttaaaacct ctcatgtctt
    21421 ctgaatttat ttcatctgta tacacttctt cttcattcat tgtagacatt ggctgattca
    21481 tcattcttct tttatttttt ttataatctt gcatttttca ttgccctccc tgtaagcgat
    21541 tcttattaag tctttggctt tttgaagcct aattaatact atgcttttat aatcttaatt
    21601 gttacttgct ttttgaattt atcaataatt aggcgtattt agtagaaaaa tgcatattaa
    21661 taaaaaaagt actactattt gaaatagtag cacttttttt gtcccgaaac accggtggct
    21721 aagtaaattc acaccctagg cactcccagg tgggtgactt gccagtaacc tttaacgtcc
    21781 aaactaacta ggcatgttat aatttaccag acacggtctc ccgtatttca tttgtaggtt
    21841 gaatttaaaa aagccttatc gtatgtttca ggatttcata ttattattat ataataaatt
    21901 ttgaattttt taaagtggtt ttatatagta attgagataa tcttattcta tcattacttt
    21961 ttcttccttt tttctttatg tatttagtta attatcgttt tttcttagct ctagttttag
    22021 tatttttttc attataattg tattcggatt cttgctcatt aatgttgtat agttcctcat
    22081 cgtatactat atcttcatca ttttctatat tttcatagcc tgcgttctta tcttctacta
    22141 attcatcttc attatcctcc atttcacctt catctgttaa ctcatcatca taatccaatt
    22201 ctttgccttc acaatctaat tcttcgtctt cataatttaa ctcttcatta ttgtcttcat
    22261 catcatcatt attactatca tcacctctta aaagacttcc taacaatttg agattattca
    22321 tactgccaag attgtttata ttggccaaag aactcaaact aaatccacct tcaccaaagt
    22381 catctggtaa aaataattct gcaaataaat ctatcttctt tttcattcct atagcttttt
    22441 caactgaatt ttttcttttt tctgggaata catctataat ttcctttatc atcatattct
    22501 ttttatcatc actatccatt tttctgaaaa aatccgtttc atcttctata tctaccatat
    22561 ctatattatt taattttttt attcctctaa ctaaatctaa aacactttct atttttatta
    22621 gtatttttct ttcatctctt gacatgtatt ttttagttct tgttattatt tccattccca
    22681 tttccaaatc ttcttcactt aaatccatga gccttaaacc tctttttatg tcatttgcat
    22741 caaaccttaa attgccatgt gtagactgtg aaagtttatc atttgcattt tttttgtttt
    22801 ttctaactct attatttcct tttgatttat ttttatcatc aaatatagag atattaccat
    22861 tactcaatgc aatcactcca atcaaaaata gtaagttatt caagacaatt cccccttata
    22921 ttatataatt gccctatatt aaagtatgtt tttcatattt taatgttcca tttttaaagc
    22981 tcagtgcata tatattatct ataagaattt ttatttatat tatatatcac tcacaattca
    23041 ccacttcaat attatttcta tatataaata gtatgaatat aattcaatat atatttatag
    23101 aaaggggttt ttttatgaaa aaattagata cagagtcatt agaaaaaatg gctaaacaaa
    23161 aaaatattga caaggataaa atagaaaaga tggctgatag ttacaaagga aaatcagaaa
    23221 atgaacttat ggaggaactt ataaaaatag gtaagaactt agatggaaga gatgaagtag
    23281 tatcaaaatt caaagcattt ttagatgaaa atcaacgcaa aaaactagat aatataatgg
    23341 aaaaaatctc agatgcagaa aatcaaagag atactaaacc agctaaaact aagaaggcta
    23401 aacctactaa aggtaagtca aattcacctg caccaaaaaa tacaccaggt agtcaacaaa
    23461 aaaaatctaa aagtttattt aagaaaacta aaaagtctaa ccctcatgag tagataaaaa
    23521 taaactattt aagtaattag ataattattt ttagttaatc tattattaaa tttgatatta
    23581 ttgacataaa aaataaggta gtgcct
//
LOCUS       DAEMAE010000042        23478 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEMAE010000042 DAEMAE010000000
VERSION     DAEMAE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.043"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..614
                     /locus_tag="KPH93_001320"
     CDS             <1..614
                     /locus_tag="KPH93_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688149.1"
                     /translation="DDDCNSIKKYGLINLIQAITLETPLSKYLKSFNIVFDVYNYLIF
                     IDEQVFSIRDYKESKEVIRLLYLKLYDDYNINAFFYVRDFKSYGGRVHEKPEILCNLS
                     KAFNNKNISEKWKNNIHNKCYSVKFKVRLEELEWYTFYDNIEEYKKDYTKKDLIRNEL
                     VENALSVIHEDYFKKDSKSELIAYLKREVLIPYSNIIEIEEIK"
     gene            901..1500
                     /locus_tag="KPH93_001321"
     CDS             901..1500
                     /locus_tag="KPH93_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1688150.1"
                     /translation="MKRPAKPIKKQQDVLDIQDYLRYKSYRNYVIFILGITTGYRAGD
                     LVKLKVREVKEALKRQEFTIWEGKKVNSKNIREKNRKPRTVEIRPKVTHILKKYIKNK
                     KDYEYMFPSRKKDYPYIGVAAISKILKEAGEYFGLYDITAHSMRKTYAYKIYIDSGKD
                     IVAVKELLGHSSIEETKAYLGLDKELYHHYSKSLDDFVR"
     gene            1697..1990
                     /locus_tag="KPH93_001322"
     CDS             1697..1990
                     /locus_tag="KPH93_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688151.1"
                     /translation="MKEIELSNKEIELIISVLDSKISSNLDKVFGDVQYSPTCKHRLR
                     KENSIIENLIDKLIDRLNGIEVEGKENVLRIDWNMEEFEGKLKEVIEEYLCNG"
     gene            1983..2276
                     /locus_tag="KPH93_001323"
     CDS             1983..2276
                     /locus_tag="KPH93_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG1688152.1"
                     /translation="MAREFSQSFYNSKAWKDCRQAIVKKYLGLCAECGKLGEEVHHIK
                     HLTPSNINDVDIALGEENLILLCKDCHSKKHKSKKDITRAGLKFNENGELISI"
     gene            2374..2763
                     /locus_tag="KPH93_001324"
     CDS             2374..2763
                     /locus_tag="KPH93_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688153.1"
                     /translation="MGGGLFKIIYEQLEKEKKIKQEVSRLKKNYKDLEKEKVKILDGL
                     VNEAAFLKISLEETREILTKEGLTEIFKQGKQEFERERLQVKIYLNFMKLYSNVMKQL
                     IDIIPSDMKQEEEDELIKFIKKGRLNK"
     gene            2760..4409
                     /locus_tag="KPH93_001325"
     CDS             2760..4409
                     /locus_tag="KPH93_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBG1688154.1"
                     /translation="MTYIEEYYQKILNGEIVACSRIKQVYKKLVQDLYNPKGNWVFDE
                     ELANRPIEFIETFCKQAQGKLGEPLKLELFQKAKHQAVWGFVDKETRFRKYQEVLDIR
                     GRKNGKTTELAADELFMLIADNEGSPEVYNIATKYEQAQKGFKECYKMVQQSKILSKH
                     IKKRKSDLYFHANYGFLQALASNSNGLDGLNSHMVTIDELAAIKNRDIYDLMKQSMGA
                     RNQPLLNCITTNGFVREGIFDAQYEYACNVLDGKIKDDRFIAFIYELDDKDEWDREEC
                     WIKANPGLGTIKKLDFLRDCVNKAKADPNFKPTVMVKDFNMKENSATAWLRWDELNNE
                     TKFNADEMGFRYGIGCFDLAETTDLASAKVLLRKRYDDNIYVLSMYWIPFERLEQKTD
                     EDKIPYILWEKQGLLRICEGNKINPYDILRWFREIKEEYDIYIPWIGFDPWHVDSSLL
                     LAYENEFGKDSMIKVRQGAYTLSAPMKELRADLKANKVIYNNNSIDKWCLSNTEIKTD
                     INGNIQPIKGVDRRKRIDGAVSLIIGYVVLKEKMSEYENMI"
     gene            4425..5579
                     /locus_tag="KPH93_001326"
     CDS             4425..5579
                     /locus_tag="KPH93_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1688155.1"
                     /translation="MNIFKSKKKNKEAPGKVAIELITDSGNGFYSWCGNLYRSDIIRS
                     IIRPKSKAVGKMTAKHIRSNETEFKTNPEIYIKFLLENPNPFMSGQILQEKMVTQLEL
                     NSNAFAVIIKDSYNIPIQIYPLNALNVEAIYEDEVLFLKFSLKNGKIVTYPYSNIIHL
                     RKDFNENDLFGTPPTKVLEPLMEVVNTTDQGVVKAIKNSNTIKWLLKFKTALRPDDIK
                     KEVKEFEKNYLQIDSDAGGAAATDSKYDAEQVKAESYVPNAAQMDKAIQRLYSFFNTN
                     EKIIQSKYTEDEWNAYYESEIEPVGLQLSNQYTEKLFTRKARSFGNEIIFEASNLQYA
                     SMSTKLNLVQMIDRGSLTPNEWRKIMNLSPIEGGNKPVRRLDTAVVEGGE"
     gene            5585..6322
                     /locus_tag="KPH93_001327"
     CDS             5585..6322
                     /locus_tag="KPH93_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBG1688156.1"
                     /translation="MANDNLNEFLKIKNSTETSSSLYFYGDIVCDEWDAWTEEDQYPL
                     SIKNFLATEQGKDLNIYINSGGGSVFAGMAIYNILKRHEGFKTVYVDGIAASIASVIA
                     LAGDKVVIPENAYFMIHKPWLGLFGAYNSDKLIKASQDLDRIEEGILNVYKDNLREGI
                     DIEEIKEKLREETWFTGKEASNYFNFEVDEKKEVAACVSDYFDKYNKMPNILKSKIDK
                     DSNKKENNNKKRVQLRLDLLKLGGLND"
     gene            6315..7583
                     /locus_tag="KPH93_001328"
     CDS             6315..7583
                     /locus_tag="KPH93_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBG1688157.1"
                     /translation="MTREEYFKKRQEMIDEAQKLLDDEVGEEGAGEENTEEAEKIANR
                     IKALDEEYERNVKARANLKALQDNVKIDPTIFNLTNNKGKIEGIEDTTVKDKQEQYKN
                     AWAKDMLGKQLNSEEQEIFNSINSAYRAEVQTSENNTILIPKTVASGIWKEIGDMYPL
                     FGDASPTFVAGDLTIIAEEDGGDDAAWYDEETEVKEDGYKLKEITLRGCELAKDITVS
                     WKLKKMSIDEFVPYITSLLAEKMGAALAKAIVDGKGKPGESDSFKPQPLGIKTALSKE
                     ASKAQIIEYTDKIAYTDITKLMSVLKKWSNGACIYANSTTIWTQLAEILDTTGKPIFI
                     PDAANSDGVGRMFGRVVKMDDSMADGEILAGNIAKGYAININENVTLYTDEHVKSRKT
                     DYLTYSLVDGNVISNKAFGMIVKKTSAVAK"
     gene            7593..7880
                     /locus_tag="KPH93_001329"
     CDS             7593..7880
                     /locus_tag="KPH93_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBG1688158.1"
                     /translation="MIVSLEEIKEYLRLEADYKEDDNLLLSFLKAAEEDLENRTGKVF
                     NENNKSELVGLYIKMYSAEQYEKRGATESNSEKVRFVLESIISQISICSRY"
     gene            7882..8220
                     /locus_tag="KPH93_001330"
     CDS             7882..8220
                     /locus_tag="KPH93_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBG1688159.1"
                     /translation="MDVGKLTQRIKIQIYGEIENDIGEITKGWSTYKKLWANKSLLRN
                     SNNYVLDKENIEYSYRFKIRYRTDITEAMRIVCNDVVYDIKHVNNIKELNKYETNIDC
                     ILYKEGVYNE"
     gene            8213..8626
                     /locus_tag="KPH93_001331"
     CDS             8213..8626
                     /locus_tag="KPH93_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG1688160.1"
                     /translation="MSNTDFNMNGLDDYTNKLFKRIVKEYPKKAEKLMNTSLGKCKGE
                     AIARTPKSSKKPKKYKRAKHMKDNWKTKVQSKNGNCTGVLKNDSPHAHLIENGWATKN
                     GGYVEGKHMLQQTMEHQRVKIDKRIEKMVDETFNL"
     gene            8638..9054
                     /locus_tag="KPH93_001332"
     CDS             8638..9054
                     /locus_tag="KPH93_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688161.1"
                     /translation="MLKIVSVKKAIVEKLKSLNIKIVANEIRSGFEKPAFFIQIIPVE
                     MSSDPNFSNSTLLINIHYFSKEKTELENLKMIDKLNVLFQDCILEIDGGELTIEEKSV
                     EIYENVLQYKFNLQVVEIIEEDESKYELMKELEMNI"
     gene            9069..10136
                     /locus_tag="KPH93_001333"
     CDS             9069..10136
                     /locus_tag="KPH93_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG1688162.1"
                     /translation="MGLPSAIIEFQRRSKTVKFRSRRGVVALILKDSTAIKKSYSINF
                     LTDINETEFTKENYDYIKLAFLGKPSKVIIEVINDSVDSERSLDDALKALRENKFNYL
                     AIPFISEEVDKTKIVNWIKIARREKEIYKAVLPSVNNANEKAIINFTTAGIKVGEKSY
                     TTAEYTARLAGILAGISLSESCTYFALDEVTEIEPAENPDEAVEKGKLILINNNGIRI
                     ARGVNSLITLSKEDTEDLKKIKIVEAIDMIQDDILQTWNENYVGKVTNKYDNKILFLS
                     AVNNYFKELQRDEVLDNSQEAYVQIDIEAHKKYLKEKGIDYSEMTEQQIKEANTGSYV
                     FVEGNITITDAMEDLKFKIYM"
     gene            10149..10598
                     /locus_tag="KPH93_001334"
     CDS             10149..10598
                     /locus_tag="KPH93_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG1688163.1"
                     /translation="MSKENIVGSSQISGTWGKLWWDGTLIAEVLSFEAKVTANREEIQ
                     FGMSKDSKITSISGEGTVKLGKVYSRGKKKLLEAWKKGEDPRSTLTSKIKDPGTPGKQ
                     AETVTINNVWFNELALAQFEKGGKIEEELSFGFTPNDSDMMDEIEEI"
     gene            10647..11030
                     /locus_tag="KPH93_001335"
     CDS             10647..11030
                     /locus_tag="KPH93_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1688164.1"
                     /translation="MENKKEMITIEDILRRKEYFAKKSEETKQLYIPSLGGNIEIAKP
                     DRELCIDVTEMENSIESDKYFVYEIVKKPNLKSEKLHTEFGCKDNPLDIVDVLFETGE
                     IADIAKIAASFAGFGVVEEVEDLKN"
     gene            11048..11221
                     /locus_tag="KPH93_001336"
     CDS             11048..11221
                     /locus_tag="KPH93_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688165.1"
                     /translation="MQMISHYLEKGVDLDKLTNLNIIERNFRIACMLYEEEEKIKLIS
                     ELIGAVFGGVKNG"
     gene            11214..12968
                     /locus_tag="KPH93_001337"
     CDS             11214..12968
                     /locus_tag="KPH93_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688166.1"
                     /translation="MARRHISAVISLKDNMSATMRGIRREQKQFQNEVRRTRNEMRSA
                     SRERMRIRMDATPAHRTIQNLRQKLAPLRTKLVKAVVIKDLATEKIERIKSNVKSFGK
                     FIARPAIKLKDETKGMIDKIKNRLTSLSTIVPVGAAIGAAGMAVKSGMELEQQQISMR
                     HFMGVGNKGKSSKELDGMSASYLKDLRNNANATPFETGEVISAGTRSLQIAGGNTKDA
                     MQMVKLAEDMAALNPGKSVGDAMEALADMNIGEMARLTEFGVKASSTDDPKEVQKKLE
                     TMYNGGANKLAESGSGLLSTIMGKLKSNIADIGLGMLEPLKPVMTGLIGFIDQASPKI
                     LEVGTKITAGIGMAIGWIQQQMPTLAPIFQTAFGAVSSIVSTVAPIIGQVIGALSPIF
                     MGLLSVASSVLAGIASAVKTVAPVVSSLISKLAPIFKNVGDTLKSMGKIFKNVFDSVM
                     KIVKKASDYIKPLVDGITGAAKGISDGVSWVAGKLAGNATGTKYWSGGLSVVGEHGPE
                     LVSMPRGSKVFTNAESKSMINKSIPNFRQVQGENTNYNITIPKIAETIVIREDADIER
                     ITSSLIKKIQMAKMGGVV"
     gene            12968..13603
                     /locus_tag="KPH93_001338"
     CDS             12968..13603
                     /locus_tag="KPH93_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG1688167.1"
                     /translation="MEMWLRQSNDAFRFPIFPASFEISGNINTSTTNVLRLGEVIICG
                     GTGLRTTEISSFFPSRQYHFCNYKDFPQPYDCVNKLKRWMEQGLILRYIITETDVNME
                     VIIESFKHGKQDGTNDVYFTLSLKEYKRIQIPSINSSDGKIEVVKNVPVTKGFETGKQ
                     KTHKVVKGDNLWSLSQKYYGNGDLYEKIVEANKELIKTADIIKEGWVLIIP"
     gene            13843..15360
                     /locus_tag="KPH93_001339"
     CDS             13843..15360
                     /locus_tag="KPH93_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1688168.1"
                     /translation="MIINRSKDSSNNSISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFNDNKLAVGNIPKTNVKYTKMFIGVTGYDTIMSAYTEASKTTKKKYMIESNLDKFN
                     VIEKGTITLNVMFEEGSNLINTSFSESMENVKNKVLVVDQYGNKISEKVDDKIFKDVG
                     VIMQKVIQQQENSTVDIESEFKGIEQTCSLKGYGDVTCITGRGVKVKDRYTGLVGLFY
                     IDTDKHNWDSNGNYEIDLDLNFQNIMDEKTAGQDEQKESSGLNGEGTLNGKEVKAEFT
                     AYYPSNNAMEGGYYQAMDGKRLVPSNNTCAAPSKLKFKTKIQAKCPGTKIDGKTYTVT
                     DRGGAIDLKNGVYRIDILMSSEKECNDFGRRKGTIIIGDGTGYTNATGKAKELISIAK
                     SKLGCKYVWGATGENTFDCSGFTQWCYKKIGISIPRTASAQSKAGKPVDLNDRSKWKA
                     GDLLCRVSGGSNNHVVMYIGNNQIIHSPQTGDVVKIQSVDSYRKGKAYTHVRRYL"
     gene            15376..15702
                     /locus_tag="KPH93_001340"
     CDS             15376..15702
                     /locus_tag="KPH93_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG1688169.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIEVNNII
                     LEKNDILIDSWMLDRQIEIFNTETNQEHKHEVKNPFIDKFELDDTVIMFKISDKFAVV
                     SKLVSL"
     gene            15702..16130
                     /locus_tag="KPH93_001341"
     CDS             15702..16130
                     /locus_tag="KPH93_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG1688170.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWNFEKDKPILENGDF
                     KIVEGNEAIKVWVYKCIKTNKYEHEIYSWGYGTELSELIGQKYSKGLTESEASRYIKE
                     ALLVNPYVLDVNISNTRFIDDLLSVDIVINTIYGEVEVNV"
     gene            16123..17178
                     /locus_tag="KPH93_001342"
     CDS             16123..17178
                     /locus_tag="KPH93_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG1688171.1"
                     /translation="MYSSQTYDVVKNRTLSNIDLDIYKGEGSFLSDMVSPVNSELAKF
                     YIELSYLHKKAFIEDNFDDFLDKRVNEFGVYRKLGTEATGEVIFEGKVGTTIQNGTII
                     SYNELLFVVIKDIVISSEIEQNTSPVQALEIGIRYNIPASTEFKLQDEINGITKIYND
                     LAFRGGTEIETDKELKERFYKIQKNQATSGNKAHYEAWALEVEGVYNAKIYPRWDGPG
                     TVKVLIFGENNQAVDSEVIERCREHIEEEMPIGPMLTVLTPSVLDISISASIKLETGY
                     TLDFVKESFLESINSYLINVNKEIIYTKVSAILASIEGIHDFSNLLLNNKAENIVFEE
                     DKVPSVTNLEFSEVVVQ"
     gene            17175..17792
                     /locus_tag="KPH93_001343"
     CDS             17175..17792
                     /locus_tag="KPH93_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG1688172.1"
                     /translation="MKLIDKLPSFYNNDITRKIQEAYDIELETLRETYDDTFDQFFVD
                     TATWGLDYWENILSIKNRFDLSIEDRRSNIKAKMRGKGTTTIEVIKAIGEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPCHLEHKFEMILFNQNDMFAGST
                     TNTGETVTIYPYSPTDITSFGELTITTGNDRSMEKVTLYPRQEVV"
     gene            17794..18795
                     /locus_tag="KPH93_001344"
     CDS             17794..18795
                     /locus_tag="KPH93_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG1688173.1"
                     /translation="MAEQKYYTLLTTIGKASIANATALGNKVDLVKLQLGDGAGTEYN
                     PTEEQTSLKNVVWEGSVNNVKIDEENPNWIVIETVIPGSVGGFMIREVGIFDSKEQLI
                     AVSKYPETYKPTADSGSVKDLVIKIILVVSNTSSVNLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLVAGGVNVSHTHIIEVADWILNN
                     ETNMYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENIKMYVYLIN
                     GNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKLSGNLSDIATSVNELITYC"
     gene            18812..20509
                     /locus_tag="KPH93_001345"
     CDS             18812..20509
                     /locus_tag="KPH93_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688174.1"
                     /translation="MATEWNFDFKAEAQPITLKAGKYKLECWGAHGKVWGGDSQSSGG
                     YSYGELTLKKETTLYVYTGATGSSNKYEKFTFNGGGLGVNNGGGGATDIRLVNGDWNN
                     EQGLLSRIIVAGGGGGAFSKTPAGKGGGFKGGNSTNDDNSSMLIVPGGTQYDGGRGYC
                     DEWDGVFGCGGGSILGLERGKYPYNSGGGGWFGGAGARNTSSGGGGSGYVLTKDSYKP
                     VGYIPTSEYWLENVGSITGGNTAKVNGYAKITLLQALPILTISSYNSTQATFKADHTD
                     PTLLTKIEVFIDDTLKETITTDLTLEKTINYTLEDNALHTLKIVVTDSNNATAEKVLS
                     ISKNIMPLPENVNLQDISTKLTEVNAGFKSGKTSIINTLALKNIEASLNNTLIELSEK
                     IKISFDSSDASVQDLMNQLTQANNTISQLNTKYKVASGRTSTLTDTTSTAYLYVNSQS
                     NPNYPINPGGWVNIKGLNFIPNIFFAECECTTNSPTQFYKYLIFATYLIPSLSDKDFV
                     ITTALRKTNSDTKFTADSQVYINNRGNTYINNQGVYVPAYRPSVSYTLYNWYAIKFV"
     gene            20523..20816
                     /locus_tag="KPH93_001346"
     CDS             20523..20816
                     /locus_tag="KPH93_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688175.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITKLHCIDIPYGS
                     IDYMQNRIVGINIETKEPILEEIPIFVTEEEKRIQELENQILIAENEKVGGIL"
     gene            20816..20998
                     /locus_tag="KPH93_001347"
     CDS             20816..20998
                     /locus_tag="KPH93_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688176.1"
                     /translation="MNINNVVVRILAERILGGGLNPLKNREFELDDVTNVEYRKAVED
                     YIIRESGVVEGAEPTI"
     gene            21032..21565
                     /locus_tag="KPH93_001348"
     CDS             21032..21565
                     /locus_tag="KPH93_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688177.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATKDNTFAELASLIGDRKKFKMGNIQLTMEQKKANL
                     EIDFEPEILIAFIGEIDYSRYNTATYYKEGVLLLEGRINSISFKILNENSIYSIVMNP
                     PYNVSTAPLGVTWYAIK"
     gene            21636..21866
                     /locus_tag="KPH93_001349"
     CDS             21636..21866
                     /locus_tag="KPH93_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG1688178.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAIQKGIF"
     gene            21886..22143
                     /locus_tag="KPH93_001350"
     CDS             21886..22143
                     /locus_tag="KPH93_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG1688179.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVILLTL
                     GVGFSVWMLGLNPSAVLQGVICWGISIGINQTYKQLKEENK"
     gene            22143..22955
                     /locus_tag="KPH93_001351"
     CDS             22143..22955
                     /locus_tag="KPH93_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1688180.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADVFRKE
                     GHKVDIIICPEKQFKTKSEEKTYKIPKVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKGLYILNSSKPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINSEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGTITKEHYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(22984..23463)
                     /locus_tag="KPH93_001352"
     CDS             complement(22984..23463)
                     /locus_tag="KPH93_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688181.1"
                     /translation="MFLNDIFDIIKFNKKSPIEQLYVIQEEEKPDIKEAEEYYRCNYR
                     FYNSLKEMDSIENIKTEKTRIKTVLDSKNNPSIPFAIAIIAAFISIFFSLLTNNIIKD
                     FFTACSVIGVFFGTYLCLICESQQKWDSRQKFYMSSLQVLDNMESKKLKERKYKKRR"
BASE COUNT         9284 a         2615 c         4720 g         6859 t
ORIGIN      
        1 atgatgatga ttgtaattct ataaaaaaat atggtctaat taatttaata caggctataa
       61 ctttagaaac tccattaagt aaatatttaa agagttttaa tattgttttt gatgtatata
      121 actatctaat ttttattgat gaacaagtat tttctataag agattataaa gaaagtaaag
      181 aagttataag attattgtat ttaaagttat atgatgatta taatataaat gctttttttt
      241 atgttagaga ttttaaatca tatggtggta gagtgcatga aaaaccagag atattatgta
      301 atttaagcaa agcatttaat aacaaaaata tatctgaaaa atggaaaaat aatatacata
      361 ataaatgcta ttctgtaaag ttcaaagtta gattagaaga gttagagtgg tatacttttt
      421 atgacaatat tgaggaatac aagaaagatt atactaaaaa agatttaata agaaatgaat
      481 tggtagaaaa tgctcttagt gtaatacatg aagattattt caaaaaagac tcaaaatcag
      541 aactaattgc atatttaaaa agagaagtat tgattcctta tagtaatata attgaaattg
      601 aagaaataaa atagaaaagt ttatataaaa ttataattta ttagattttt gatacaactg
      661 aattatggag catgaaaatt attttaatga ataaacttat tttatgaaag gctagattta
      721 ataatctagt ctttttttat acttaatatt gaaaggagtt gttatattat atgggaagat
      781 ataagttgta taatcgataa agaatgtgaa ctatctaaag atggaaagag ctaaaaaaat
      841 aaatggaaat tattcctagg tttaataaat ctaaagaaat ggggggaaga aatgtaaaag
      901 gtgaaaagac ccgcaaagcc aataaaaaag caacaagatg tactcgacat ccaagattat
      961 ctaagatata aaagttatag aaattatgta atctttatac ttggtataac aacaggatat
     1021 agagcaggag atttagttaa attaaaagta agagaagtaa aagaagcttt aaaaagacaa
     1081 gaatttacta tttgggaagg taagaaagta aatagcaaga atataagaga aaaaaataga
     1141 aagcctagga cagtagaaat aagaccaaaa gtcacacata ttttaaaaaa atatataaaa
     1201 aataaaaaag attatgagta tatgtttcca tcaagaaaaa aagattatcc ttatattgga
     1261 gtagctgcaa ttagtaaaat tcttaaagaa gcaggagaat attttggatt atatgatata
     1321 acagcgcata gtatgcgtaa aacttatgct tataaaatat atattgatag tggaaaagat
     1381 attgtggcag ttaaagaatt gctagggcat tcttcgatag aagaaacgaa ggcatattta
     1441 ggtttagata aagagctata tcatcattac agtaaatcgt tagacgattt tgtgagatga
     1501 tattttttta tttgcctgtt tgaatgtcta aaaatttggt gtattactat ttaaggtgtc
     1561 aaattctcta tataagaagt aggaaaaatt taatttgaat gtttgattta ctaagaaaat
     1621 agaggattca aatgaaaaaa tacgaactat atttattaaa aatataataa tattcgtatt
     1681 gaaaagaggt gtttttatga aagaaataga gttaagtaat aaagagattg aactgattat
     1741 ttcagtatta gattctaaaa tttctagcaa cttagataaa gtttttggtg atgttcaata
     1801 tagtcctact tgtaaacata gacttagaaa agaaaattct atcatagaaa atttaataga
     1861 taaattaata gataggctta atggaataga agttgaagga aaagaaaatg ttttaaggat
     1921 agattggaac atggaagaat ttgaggggaa gcttaaagaa gttattgagg aatatctttg
     1981 taatggctag agagttcagt caaagctttt ataatagtaa agcatggaaa gattgtaggc
     2041 aagcaattgt taagaagtat ctaggcttgt gtgctgagtg tgggaagtta ggagaagaag
     2101 ttcatcacat aaaacattta actcctagta atataaatga tgttgatata gccttaggtg
     2161 aggaaaattt aatattgcta tgtaaggact gtcatagtaa aaaacataag agtaagaaag
     2221 atattactag agcaggatta aaatttaatg aaaatgggga attgatttcg atttagaaaa
     2281 aaatagcata tcccccccta aaaacgaccc tggggggctg atttcaaata ccgatgtccc
     2341 cacgtcaatt ttcctccgca tgaaaaaatc agaatgggag gggggttatt taaaataatt
     2401 tacgaacaat tagagaaaga aaaaaagata aaacaagagg taagcagatt aaagaaaaac
     2461 tataaagatt tagaaaaaga aaaagttaaa attttagatg ggttagtaaa tgaagcagct
     2521 tttttaaaaa tatctttaga agagactaga gaaattttga caaaagaggg tttgactgaa
     2581 attttcaagc aaggcaagca ggaatttgaa agggaaagac ttcaagttaa gatatattta
     2641 aattttatga aactttattc taatgtcatg aagcaactaa ttgatattat tccaagcgat
     2701 atgaaacagg aagaagagga cgaacttatt aagtttataa aaaaaggtag acttaataaa
     2761 tgacctacat tgaagagtat tatcaaaaaa tattaaatgg agaaatagtt gcttgtagca
     2821 gaattaagca ggtgtacaaa aagcttgtcc aagacctata taatccaaaa ggaaattggg
     2881 tttttgatga agagcttgct aatagaccta tcgagtttat agagactttt tgtaagcaag
     2941 cacaaggtaa gttgggtgaa cctttgaaac ttgaattatt ccaaaaagcc aagcatcaag
     3001 ctgtgtgggg ttttgttgat aaagagacta ggtttaggaa atatcaagaa gtacttgata
     3061 ttcggggtcg taaaaatggc aagactacag aattggcagc ggatgaatta tttatgttaa
     3121 ttgcagataa tgaaggttcg ccagaggttt ataatattgc aactaaatac gaacaggcac
     3181 aaaaagggtt taaagagtgc tacaaaatgg tacagcaatc taaaatttta tctaagcata
     3241 ttaaaaaaag aaaatcagac ctctattttc acgctaatta tggtttttta caggcacttg
     3301 ctagcaatag caatgggctt gatggattaa actcgcatat ggtaactata gatgaattgg
     3361 cagctataaa aaatagagat atttacgacc taatgaaaca atctatgggt gcaagaaatc
     3421 aacccctttt aaattgcatt accacaaatg gttttgtgag agagggtatt tttgatgcac
     3481 aatatgagta tgcttgcaat gttttagatg gaaaaataaa agatgatagg tttatagctt
     3541 ttatttatga gttagacgat aaggatgaat gggacagaga agaatgttgg ataaaagcta
     3601 atccaggttt agggactata aaaaagcttg attttttaag agattgtgtt aataaagcta
     3661 aggcagaccc aaactttaag cctactgtca tggtgaaaga tttcaatatg aaagaaaatt
     3721 ctgcgactgc ttggttaaga tgggatgaac taaacaatga aactaaattc aatgcagatg
     3781 aaatgggatt tagatatgga ataggttgct ttgacttagc tgaaactaca gacctcgcat
     3841 ctgctaaagt tttgttaagg aaaagatatg atgataatat ttacgttctt tctatgtact
     3901 ggattccatt tgagcgatta gaacaaaaaa ctgatgaaga taaaattccg tacattttat
     3961 gggaaaagca aggtttgtta agaatctgcg aaggaaataa aataaatcca tatgacatct
     4021 tgcgatggtt tagagaaatc aaagaggagt atgacattta tattccatgg attgggtttg
     4081 acccttggca tgttgactcg agtttgctgt tggcttatga gaatgagttt ggaaaagatt
     4141 ctatgattaa ggttaggcaa ggggcttata ctttatctgc tcctatgaaa gaattaagag
     4201 ctgatttaaa agctaataaa gttatttata ataataattc tattgataaa tggtgcctta
     4261 gtaatacaga aataaaaacg gatataaatg gaaatataca accaataaaa ggtgttgata
     4321 ggcgaaaacg tattgatgga gctgtatctt tgataattgg ttatgttgtt ctgaaagaaa
     4381 aaatgtcaga atatgaaaac atgatttagg tgagggggtg aaaaatgaat atatttaaat
     4441 ctaagaagaa aaataaagaa gctcctggga aagttgctat agagcttatt acagattcgg
     4501 gaaacgggtt ttatagttgg tgcggcaatt tatacagaag cgacattata agaagtatta
     4561 tacgaccaaa aagtaaagct gttggcaaaa tgacagctaa acatattaga agtaatgaga
     4621 ctgaatttaa gaccaatccg gaaatttaca ttaaattttt gcttgaaaat cctaatccat
     4681 tcatgagtgg tcaaatactt caagagaaaa tggttactca gttagaactt aatagtaatg
     4741 cttttgctgt aattattaaa gatagttata atataccaat tcagatttac cctcttaacg
     4801 ctttaaatgt tgaagctatt tatgaagatg aagttttgtt tttgaaattt tcgcttaaga
     4861 atgggaaaat agttacttat ccatattcga acataattca tctaagaaaa gattttaacg
     4921 aaaatgattt atttggaaca cctccaacta aagtgcttga gccgcttatg gaagttgtaa
     4981 acacaacaga ccagggagtc gtaaaagcta tcaaaaatag taacacaatc aaatggttat
     5041 taaaatttaa aacagcactt agacccgacg acataaagaa agaggtcaag gaatttgaaa
     5101 aaaattattt gcaaatagac tcagacgcag gcggagctgc tgcaactgat tcgaaatatg
     5161 atgctgaaca ggtcaaagct gagagttatg ttcctaatgc tgcacaaatg gataaagcaa
     5221 tacagagatt atattcgttt tttaatacaa atgagaaaat aattcaaagt aaatatacag
     5281 aagacgaatg gaatgcttat tatgagtctg aaattgaacc agttggattg caattatcta
     5341 atcagtatac agaaaaactt tttactagaa aagctcgaag ctttggaaat gagataattt
     5401 ttgaagcttc taatttacag tatgcaagta tgtccactaa attaaacttg gttcaaatga
     5461 ttgacagggg ttcacttact ccgaatgaat ggcgtaaaat aatgaacctt tcgccaatag
     5521 agggtggaaa caagccagtt cgtcgcttgg atactgctgt tgtggaagga ggtgagtaaa
     5581 ataaatggct aatgacaatt taaatgagtt tttaaaaatc aaaaactcaa cagaaacaag
     5641 ttcaagcctc tatttctatg gagatattgt ttgtgatgag tgggacgctt ggacagaaga
     5701 agaccagtac ccactttcta ttaaaaactt tttagcgact gaacaaggaa aagatttaaa
     5761 tatttatatt aattccggtg gtgggtctgt atttgcgggc atggcaatat ataatatact
     5821 aaaaagacat gaagggttta aaactgttta tgtggatggt attgctgcaa gtattgcgag
     5881 tgttatagca ttagcaggtg ataaagttgt aatccccgaa aatgcttatt ttatgattca
     5941 taaaccttgg ttgggtttat ttggtgctta taattcagac aaattaatta aagcatcaca
     6001 agatttagac aggatagaag aaggcatttt aaatgtgtat aaagataatt tgagagaagg
     6061 aattgatatt gaagaaataa aagaaaagct aagagaagaa acttggttca ctggtaaaga
     6121 agcatcaaat tattttaatt ttgaggttga tgaaaaaaaa gaagttgctg cttgtgtgag
     6181 tgattatttt gataagtaca ataaaatgcc taacatttta aaaagtaaaa tagacaaaga
     6241 tagtaataaa aaagaaaata ataataaaaa aagagttcag ctaaggctgg acttattaaa
     6301 attagggggt ttaaatgact agagaagaat attttaagaa aagacaagaa atgatagacg
     6361 aagcacaaaa attacttgat gatgaagttg gggaggaagg agcaggagag gaaaatactg
     6421 aagaagctga aaaaatagct aataggataa aagctttaga cgaggaatat gaaagaaatg
     6481 taaaagctag ggcgaattta aaagcattgc aggataatgt caaaatcgac cctactattt
     6541 ttaatttaac taataacaaa ggtaaaatag aaggtataga agacacaact gttaaggata
     6601 aacaagaaca atataaaaat gcttgggcta aagatatgtt aggaaagcag ttaaattcag
     6661 aagaacagga gatatttaat agtattaact cagcatatag agcggaagtt caaacaagcg
     6721 aaaataatac tattttaatt cctaaaactg ttgcttcggg tatatggaaa gaaattggtg
     6781 acatgtatcc gttattcgga gatgcttctc caacttttgt agcaggtgat ttgacgatta
     6841 tagcagaaga agatggtggt gatgatgctg cgtggtatga tgaagaaaca gaagttaaag
     6901 aggatggtta taagctaaag gaaataacct taagaggttg tgagcttgca aaagatataa
     6961 ctgtgtcgtg gaaattgaaa aaaatgagta ttgatgaatt tgttccatat ataactagtc
     7021 ttttagctga aaaaatgggt gcagcattgg cgaaagcgat tgtagatgga aaaggaaaac
     7081 caggggaaag tgattctttt aaacctcagc cgcttggaat aaaaactgct ttaagcaagg
     7141 aagctagtaa ggcacaaata atagaatata ctgataagat agcttataca gatataacga
     7201 aattgatgtc tgttttaaag aaatggagca atggagcttg tatttatgct aatagcacaa
     7261 ctatatggac acaattagct gaaatattag atacaactgg aaagcctatt tttatccctg
     7321 acgctgcaaa tagtgacgga gttggaagga tgtttggcag ggttgttaaa atggatgaca
     7381 gcatggcaga tggtgagatt cttgctggaa atatagcgaa aggttatgcg ataaatataa
     7441 atgagaatgt aactttatat acagatgaac atgtaaaaag tagaaaaaca gattatttaa
     7501 cttattcatt agttgatggc aatgttataa gtaataaagc ttttggtatg atagttaaaa
     7561 aaactagtgc agttgcgaag taggtgtttt gaatgattgt atcattagaa gaaataaaag
     7621 aatatttgag attagaggca gattataaag aagatgataa tctgcttttg tcttttttga
     7681 aagcagcgga agaagatttg gaaaatcgaa caggaaaagt ttttaatgaa aataacaaat
     7741 ctgagcttgt tggcttatat ataaaaatgt attctgcaga gcagtacgaa aaaagagggg
     7801 caacagaaag taatagcgaa aaagttagat ttgttttaga aagtataata tctcaaattt
     7861 ctatatgtag taggtactaa aatggacgtt ggaaaattaa ctcagagaat aaaaatacaa
     7921 atttatggag aaattgaaaa cgacatagga gaaattacaa aaggatggtc tacgtataaa
     7981 aaactttggg ctaacaaatc gttgcttaga aatagtaata attatgtgtt agataaagaa
     8041 aatatagagt attcttacag atttaaaata agatatagga ctgatataac agaagctatg
     8101 agaatagttt gtaatgatgt tgtctatgat ataaagcatg taaataatat aaaagagcta
     8161 aataaatatg aaacaaatat tgattgtatt ctttataaag aaggtgttta caatgagtaa
     8221 tacagatttt aatatgaatg gattagatga ttatacaaat aaactattta agcgtattgt
     8281 aaaagaatac cccaaaaaag ctgagaaact tatgaatact agcttaggga agtgcaaagg
     8341 agaagctatt gctaggactc ctaaatcaag taaaaagcct aagaagtata aaagggctaa
     8401 gcacatgaaa gataattgga aaactaaagt gcagtctaaa aatgggaatt gcacaggtgt
     8461 tttaaaaaat gattctccac acgcacattt aatagaaaat ggttgggcaa caaaaaatgg
     8521 aggctatgtt gaagggaaac atatgttgca acagacgatg gagcatcaga gagtgaaaat
     8581 tgataaaagg atagagaaaa tggttgatga aacctttaat ctttagaggg ggtaagagtg
     8641 ttaaaaattg tttctgtaaa aaaagctata gttgaaaagc ttaagtcttt aaatataaaa
     8701 atagttgcaa atgaaataag aagcgggttt gaaaagcctg ctttttttat tcaaattatt
     8761 cctgttgaga tgtctagtga cccgaatttt tcgaacagca cattacttat taatatacat
     8821 tatttttcta aagaaaagac agagttagaa aatttaaaaa tgattgataa gcttaatgta
     8881 ttgtttcaag attgtatttt agagattgac gggggcgaat tgactataga agaaaaaagt
     8941 gtagaaatat atgagaatgt tttacaatac aaatttaatt tgcaagtagt tgaaattata
     9001 gaagaagacg aaagcaaata tgagcttatg aaagagcttg aaatgaatat ttagaaggag
     9061 gttttagttt gggattacca agtgcaataa ttgagtttca aaggcgttca aagactgtta
     9121 aatttagaag tcgaagaggt gttgtagctt taatacttaa agattcaaca gctataaaga
     9181 aatcttattc tatcaatttt ttaacagata taaacgaaac tgaatttaca aaagaaaatt
     9241 atgattatat aaagcttgca tttttaggaa aacctagcaa ggttattatt gaagttatta
     9301 atgattcagt tgattctgaa aggtctttag acgatgcttt gaaggcttta agggagaata
     9361 aatttaacta tttagctatt ccttttataa gtgaagaagt tgacaaaact aaaatagtta
     9421 attggataaa aatagctaga agggaaaaag agatatacaa agctgtgttg ccaagcgtta
     9481 ataatgctaa cgagaaagca attataaact tcacaacagc agggataaaa gttggggaaa
     9541 agtcttatac aacagcagaa tatacagcta gactggcagg tattttggca ggtatatcac
     9601 tttcagagag ctgcacgtat tttgctttag atgaagttac agagatagaa cctgctgaaa
     9661 atcctgatga agctgtagaa aaaggtaaat tgattttaat aaataataat ggaataagga
     9721 tagctagggg tgtaaattca ttaataactt taagtaaaga ggatacagaa gacttgaaga
     9781 aaataaaaat agttgaagct attgacatga tacaagatga tattctgcaa acctggaacg
     9841 agaattatgt tgggaaagta actaataaat atgataataa aatattgttt ttatctgctg
     9901 taaacaatta ttttaaagag ctgcaacgtg atgaagtact tgataatagc caagaagctt
     9961 atgtacagat agatatagaa gcacataaaa aatatctaaa agaaaaagga attgattata
    10021 gtgaaatgac tgaacagcaa ataaaagaag ctaatacggg ttcttacgtt tttgtagaag
    10081 gaaatattac catcactgac gctatggagg atttgaaatt taaaatatat atgtaagaag
    10141 gtgagtagat gagcaaagaa aatatcgtag gaagtagtca aatttctggt acatggggaa
    10201 agctttggtg ggatggaact ttaattgctg aggtgcttag ttttgaagct aaagttacag
    10261 caaatagaga agagattcag tttggaatga gtaaagattc taaaataaca tcgataagtg
    10321 gcgaagggac tgtaaaactt ggaaaagtgt attccagggg gaagaaaaaa ttattggaag
    10381 cttggaaaaa aggagaagac ccacgaagta cacttacaag taagataaaa gaccctggta
    10441 cacctggaaa acaagctgaa acagttacta ttaacaatgt ttggttcaat gaattagctt
    10501 tggcgcaatt tgaaaaaggt gggaaaatcg aggaagagct gagtttcgga tttactccga
    10561 atgattctga tatgatggac gaaatagaag aaatttaagg atagttttta ctatcctttt
    10621 ttatataaat ataggaggat tttaaaatgg aaaataaaaa agaaatgata acaatagagg
    10681 atattttaag aagaaaagaa tattttgcaa aaaaaagcga agaaactaag caattataca
    10741 ttccttcact tggcggaaat atagagattg caaaaccgga tagagagcta tgcattgatg
    10801 ttacagaaat ggaaaactca atcgaaagtg ataagtattt tgtttatgag atagttaaaa
    10861 agccaaattt aaagagtgaa aaattgcata cagagtttgg gtgcaaagat aatcctcttg
    10921 acatagtcga tgtattgttt gagacaggag aaattgctga tattgcaaag attgctgcat
    10981 cattcgcggg atttggtgtt gtggaagagg ttgaagactt aaaaaactaa ttaaaagcga
    11041 tgttgaaatg caaatgatta gccattactt agaaaaaggt gttgatttgg acaaattgac
    11101 taatttaaat ataatagaaa gaaattttcg catcgcttgt atgctatatg aagaagaaga
    11161 aaaaataaaa cttatttctg agctgatagg agctgtgttt gggggtgtaa aaaatggcta
    11221 gaaggcatat aagtgcagtt atatctctaa aggacaacat gagtgcgact atgagaggga
    11281 ttagaagaga gcaaaaacaa tttcaaaatg aagttaggcg gacacgtaac gagatgcgtt
    11341 cggcgagtag agagcgtatg cgtataagaa tggatgcgac accagcacac cgaactatac
    11401 agaatttaag acaaaaactt gcacctcttc gaactaagct tgtaaaagct gttgttataa
    11461 aagatttagc gactgaaaag atagaaagaa taaaatcgaa cgtaaaatct tttggaaaat
    11521 ttatcgcaag accagctata aaattaaaag atgaaacaaa aggaatgatt gataaaataa
    11581 aaaatcgact tactagctta tcaactattg ttcctgttgg ggctgcgata ggggctgcgg
    11641 gtatggctgt taaaagcggt atggaactag agcaacaaca aataagtatg cgtcacttca
    11701 tgggagttgg aaataaagga aaatctagca aagaacttga cggaatgagc gcaagctatt
    11761 taaaagattt aagaaataat gcaaatgcga cgccatttga aactggagaa gttatatcag
    11821 caggaactcg ttccttgcaa atagcgggtg gaaatacaaa agatgctatg cagatggtaa
    11881 aattagctga agatatggca gcactaaatc caggcaagag cgtcggggat gctatggaag
    11941 cacttgcgga catgaacatt ggagaaatgg caagacttac ggagtttgga gttaaggcaa
    12001 gcagtacaga cgacccaaaa gaagtacaaa agaagcttga aacaatgtat aatggtggag
    12061 ctaataagct tgcagaaagt ggttcgggac tactttctac gataatgggt aaattaaaat
    12121 ctaatattgc agatattgga cttggtatgt tggaaccttt aaaacctgtt atgactggct
    12181 taattggctt tatagaccaa gctagcccta aaatattaga agtaggtaca aaaataacag
    12241 ccggaatagg tatggcgatt gggtggatac agcaacaaat gccaaccttg gctccgattt
    12301 ttcaaacagc ttttggagct gtatcttcga ttgtatcaac tgttgcccca ataatcggtc
    12361 aagtcatagg agctttaagc cctattttta tgggactgct ttctgtggct tcgtctgtat
    12421 tagcagggat tgcttctgct gttaaaactg tagctcctgt tgtaagttct ttaatttcta
    12481 aattagcacc aattttcaaa aatgttggtg ataccttaaa atctatgggt aaaattttta
    12541 aaaatgtttt tgatagtgtt atgaaaatag ttaaaaaagc gtctgattat ataaaacctt
    12601 tagttgatgg aataacagga gcagcaaaag gtataagcga tggcgtcagt tgggttgctg
    12661 gaaaactagc tggaaatgca actggaacga aatattggtc gggcggactt tctgttgtag
    12721 gcgaacatgg accagaactt gtatctatgc cacgtggtag caaagttttt acaaatgcag
    12781 aaagtaaatc tatgattaat aaaagtattc ctaattttag acaagtgcaa ggagaaaaca
    12841 caaattataa tataacaatt cctaaaattg ctgaaacaat agtgataaga gaagatgctg
    12901 acattgaaag aataacatca agtttaataa aaaaaataca aatggcgaaa atgggtggtg
    12961 tcgtttaatg gaaatgtggc ttagacaatc gaatgatgcc tttagattcc cgatatttcc
    13021 agcctctttt gaaataagcg gaaatataaa tacaagtaca acaaatgtac taaggctcgg
    13081 agaagtaatt atctgtggtg gtacaggact tagaacaaca gagataagca gctttttccc
    13141 aagtagacaa tatcattttt gcaattataa agattttccc caaccatatg attgtgtaaa
    13201 taaattaaaa aggtggatgg agcaggggtt aattttaagg tatataataa ctgaaactga
    13261 tgtgaatatg gaggttatta ttgaaagctt caaacatggc aagcaagatg gcacaaacga
    13321 tgtttacttt acactaagct taaaagagta taaaagaata cagataccta gcataaattc
    13381 ctcagatggg aaaatagaag tggtaaaaaa tgtaccagtt acaaaaggtt ttgaaactgg
    13441 aaaacaaaaa acacataaag tcgttaaagg tgataacctt tggagcttgt cgcaaaaata
    13501 ttatggaaat ggggatttat acgaaaaaat tgttgaagca aataaggaat taattaaaac
    13561 agctgacata ataaaagagg gttgggtgtt aataattcct tagcttggag gtgatttata
    13621 attaacaata taaagttaaa ggtacacata aaaagtggta atatctatga tataactgat
    13681 atagttgaaa aagtaacttg gagtggtgat tataagtcac catcaaggac attagaattt
    13741 tctatagtac agtcagcttc tgatattaac tttcagcaaa ttaatatacc aatagctagt
    13801 acagtctgtt tctatgtaga tgagaaagaa ctctttagag gaatgataat taataggtct
    13861 aaagattcaa gtaataatag tattagtttt gtttctaaag atatgggatt cttattaact
    13921 caaagcgaag tatcatacaa ttttaaagat aaattagttg aagatattgc aaaacaagta
    13981 tttaatgaca ataaacttgc ggttggaaac atacctaaaa ctaacgttaa atatacaaaa
    14041 atgtttattg gtgtaactgg ctatgatact ataatgagtg catatacaga agctagtaag
    14101 acaacgaaga aaaaatatat gatagagtct aatttagata aatttaatgt tattgaaaaa
    14161 gggactatta cactaaatgt tatgtttgaa gaagggtcta atcttattaa tacgagcttt
    14221 tcagagagta tggagaatgt aaaaaacaag gtattagtag tagaccagta tgggaataaa
    14281 ataagtgaaa aggtagacga taaaattttc aaagatgttg gagtaataat gcaaaaagtt
    14341 atacaacaac aagaaaatag tactgtagat atagaaagtg agtttaaagg aatagagcag
    14401 acttgcagtt taaaagggta tggtgatgtg acttgtataa ctggcagagg tgtaaaggtt
    14461 aaggaccgtt atacaggtct tgtaggtcta ttttatatag acacagataa acataattgg
    14521 gacagcaacg gaaattatga gatagacttg gatttaaatt ttcaaaacat aatggatgaa
    14581 aaaacagccg ggcaggacga acagaaggaa agttctggtt tgaatggaga agggacatta
    14641 aatggaaaag aagtaaaagc agaatttaca gcgtattatc cttcaaacaa tgctatggag
    14701 ggtggatact atcaagctat ggatggtaaa agacttgtac cttcaaacaa tacttgtgct
    14761 gcacctagta aacttaaatt taaaacaaaa attcaagcaa aatgtcctgg aactaaaatt
    14821 gatggtaaaa cttatacagt aacagataga ggcggagcga ttgacttaaa aaatggagtg
    14881 tatagaatag acatattaat gtctagtgaa aaagaatgta atgattttgg aagaagaaaa
    14941 ggaacaataa taattggaga tggtacagga tatacaaatg cgacaggaaa agcaaaagaa
    15001 ttaattagca tagcaaaaag taaattaggt tgtaagtatg tttggggggc aactggggag
    15061 aatacattcg attgcagcgg gtttactcag tggtgctaca aaaagatagg gataagtatt
    15121 cctcgtactg cttccgcaca aagcaaagca ggtaaaccag tagatttgaa tgatagaagc
    15181 aagtggaaag caggagattt attgtgtagg gtcagcggag gaagtaacaa ccatgttgtg
    15241 atgtacattg gaaataatca aataatccat tctccacaaa caggtgatgt ggtgaaaata
    15301 cagtctgttg actcatatag aaaagggaaa gcatatacac atgtcagaag atatttataa
    15361 aaggatggtg ataaagtggc taatccaatc aatgaattta tagggataat aagagaagaa
    15421 ggaaaatatc acaatcaacc ttcttttttt attggaaaaa ttaaaagtaa attaccagat
    15481 ttaaaaatag aggtaaataa cattatatta gaaaaaaatg atattttaat agatagttgg
    15541 atgcttgaca gacagataga aatattcaac acagaaacaa atcaagagca taaacatgaa
    15601 gttaaaaatc catttattga taaatttgaa cttgatgaca cagtaataat gtttaaaata
    15661 agtgataaat ttgctgttgt aagtaagttg gtgagcttat aatgagtaca atatttcctt
    15721 ttataggtgt cccagaggat tatatcttac ctaaaacaga agaattgcca atctttcgtg
    15781 aagtggcttg gaattttgaa aaggataaac ctattttaga aaatggagat tttaagattg
    15841 ttgaaggcaa tgaagctata aaggtttggg tgtataaatg tataaaaact aataagtatg
    15901 agcatgagat ttattcatgg ggttatggaa cagaactatc tgaattgata ggtcaaaaat
    15961 acagcaaagg acttacagaa agtgaagcta gtaggtatat aaaagaggct ttattagtta
    16021 atccatatgt tttagatgta aatattagta atacaagatt tatagatgat ttgctaagcg
    16081 tagatatagt cataaacacg atttatgggg aggtggaagt taatgtatag tagtcaaacc
    16141 tatgatgttg ttaaaaatag gaccttatct aatatagatt tagatatcta taagggtgag
    16201 ggttcttttt taagtgatat ggtatctcct gttaattcag agcttgcaaa attctatata
    16261 gaactttcat atcttcataa gaaagctttt atagaagata acttcgacga ttttttagat
    16321 aagagagtta acgaatttgg agtatataga aagctaggaa cagaagctac aggagaagta
    16381 atattcgagg ggaaggttgg aacaactatt caaaatggaa ctattatatc ttacaatgag
    16441 ctattattcg tagtaattaa agatatagta attagttcag aaattgaaca aaatacaagc
    16501 cccgtacagg ctttggaaat tggaattaga tataatatac ctgcaagtac agaatttaag
    16561 ctacaagacg aaataaatgg tataacaaaa atttacaatg atttggcttt tcgaggtggt
    16621 acagaaatag aaacagacaa agaattaaaa gaaagattct ataagataca gaaaaatcaa
    16681 gctacaagtg gaaataaggc acattacgaa gcatgggctt tagaagttga aggagtatat
    16741 aacgctaaaa tttatccaag atgggatggt ccaggaactg taaaagtttt aatctttgga
    16801 gaaaataatc aagctgttga ctcggaggta attgaaagat gtagagagca tattgaggaa
    16861 gaaatgccaa taggtcctat gttaaccgtt ttaactccaa gcgttttaga tataagtata
    16921 agtgcatcta taaaattaga aacgggatat acattagatt ttgtaaaaga aagcttctta
    16981 gagagtatta atagctattt aataaatgtt aataaagaaa taatttacac taaagtaagt
    17041 gcaatacttg catctattga gggcatacac gactttagta atttactatt aaataataaa
    17101 gctgaaaata tagtatttga agaggacaaa gtaccaagtg ttacaaattt agaatttagt
    17161 gaggtggtag ttcaatgaaa ttaattgata agctaccttc tttttataac aatgatatta
    17221 ctagaaaaat acaagaagct tatgacatag aactagaaac acttagagaa acatatgacg
    17281 atacatttga ccaatttttt gttgatacag caacatgggg acttgattat tgggaaaata
    17341 ttttatctat taaaaataga tttgatttaa gtatagaaga tagaagaagt aatataaaag
    17401 caaaaatgag aggtaagggt acaacaacaa tagaggttat aaaagcgata ggagaagctt
    17461 acacaaagac taacgttgat gtagaagtat ttagtaactt atttagtttt acactaagtt
    17521 ttataacaaa taattgtagt tataacacta ttttagaatt agataagaaa atagaagaaa
    17581 taaaaccatg tcatttagag cataaatttg aaatgatttt atttaatcag aatgatatgt
    17641 ttgcaggttc tacaacgaat acaggtgaaa cagttactat atatccttac agtccaactg
    17701 atataacttc ttttggagaa ttgactataa ctacaggcaa tgacagaagt atggaaaaag
    17761 taacattata tcctagacag gaggtggtat aggttggcag aacaaaaata ttatacactt
    17821 ttgacaacga taggaaaggc atctattgca aatgcaacag ctttaggcaa taaagtagat
    17881 ttagttaaat tgcagcttgg tgatggagca gggacagaat ataatccaac agaagaacaa
    17941 acgtctctaa aaaatgtagt ttgggaaggt tctgtaaata atgtaaagat agacgaagaa
    18001 aatccaaact ggatagtaat agaaactgta atcccaggta gtgtgggtgg atttatgata
    18061 agagaagtag gaatatttga ttcaaaagaa caactaattg cagtatctaa atatccagaa
    18121 acatacaaac caactgctga ctcggggagt gtaaaagact tggttataaa aattatttta
    18181 gtagtttcca atacttcaag tgtaaatttg aaagtagacc caacagtaat tttagcaact
    18241 ttaaaagata tacaagaatt agacactaaa attgatacaa ctaagacaga attaacaagc
    18301 aacatagaaa ctgctaaaac agagttaaac actagaattg acacagaaaa tgagaaacaa
    18361 aatattaaaa ttgaccaact tgttgcaggt ggtgtaaatg tatctcatac gcatattata
    18421 gaggtggctg attggatttt aaataatgaa actaatatgt atgaagtaac tataaatcat
    18481 ccactattga ctaaaagaat actaatagct ttatatgatg aaattggtga agcacttaca
    18541 ccaaacgcta gggctattga tgataatagt attcttgtta gaaatgaaga aaatattaaa
    18601 atgtatgtat atttgataaa tggaaatgca gaaactcatt ttataaatgc gactgtagat
    18661 gataacagag tatctgaaat gactacttat tcgtctaaga aaatcgaaga tagattggtt
    18721 aatatagaag aaaaattgag tggaaattta tctgatattg caacaagtgt aaatgagttg
    18781 ataacttatt gttagagagg agagtgagaa agtggctaca gaatggaatt ttgattttaa
    18841 ggcagaagca caaccaatta ctctaaaagc aggtaaatat aaattagagt gttggggagc
    18901 tcatggaaag gtttggggtg gagattctca atctagtgga ggatactctt atggggaact
    18961 tacattaaaa aaagaaacta cattatatgt atatacaggt gctactggtt ctagtaataa
    19021 atatgaaaaa tttacattta atggaggagg acttggtgtt aataatggag gaggtggagc
    19081 aactgatata agacttgtca atggagattg gaataatgaa caaggtcttt tgtctaggat
    19141 aattgttgct ggtggtggtg gaggcgcatt tagtaaaact cctgctggca aaggtggtgg
    19201 ttttaaaggc ggaaatagta ctaatgatga taacagttcg atgcttattg tacctggtgg
    19261 tacacaatat gatggaggta gaggttattg tgatgaatgg gatggtgttt ttggttgtgg
    19321 tggaggttct attcttggtt tagaacgagg taaataccct tataattcag gtggaggtgg
    19381 atggtttggt ggtgcaggtg ctagaaatac ttcaagtggt ggaggtggaa gtggctatgt
    19441 attaactaaa gatagctata aacctgttgg atatatacct acatctgaat attggttaga
    19501 aaatgttggt agtattactg ggggtaatac tgctaaagta aatggttatg ctaagataac
    19561 attactgcaa gcattaccaa ttttaactat atcttcttat aattccacac aagcaacatt
    19621 taaagcagac cacacagacc ctacgctact aactaagata gaagtattta tagatgatac
    19681 attaaaagaa actataacaa cagatttaac tcttgaaaaa acaattaact atacactaga
    19741 agataatgca ctacacacac ttaagatagt cgttacagac agtaataatg ctacagcaga
    19801 aaaagtatta agtataagta agaatataat gccactacct gagaatgtga atttgcaaga
    19861 tatatcaaca aaattaacag aagttaacgc aggatttaaa agtggcaaaa caagcattat
    19921 aaatacttta gcattaaaga atatagaagc aagtttaaat aatacattaa tcgagttatc
    19981 agagaaaata aaaataagtt ttgatagttc agacgctagt gtgcaggatt tgatgaatca
    20041 gttgacacaa gctaacaata ctatatcaca gttaaataca aaatataaag ttgcatcagg
    20101 gcgtaccagt acattaacag acacaacatc gactgcttat ttatatgtta atagccagtc
    20161 taatcctaat tatccaataa acccaggagg gtgggttaat attaaaggat taaattttat
    20221 tccaaatatc ttttttgctg aatgcgagtg tacgactaat agcccaacac aattttataa
    20281 atacttaatc tttgccacct atttaatccc atcactttct gataaagatt ttgtaattac
    20341 aaccgctctt agaaaaacaa attcagatac caaatttaca gctgatagtc aggtttatat
    20401 aaataataga ggtaatactt atataaataa ccaaggtgtc tatgttcctg cttatagacc
    20461 ttctgtgtct tatactttgt acaattggta tgctataaaa tttgtataaa tggggtgata
    20521 aaatgaatag agcaaataga ataatttacg accaaacagg caaaatactt ctccaaacag
    20581 gagaagcaac aggagatata ttagaacatg atacaataac taaattacat tgcattgata
    20641 ttccatacgg aagtatagat tatatgcaaa ataggattgt aggtataaat atagaaacga
    20701 aagaaccaat tttggaagaa ataccaatat tcgttacaga agaagaaaag agaatacaag
    20761 agttagaaaa tcaaatatta attgcagaaa atgaaaaagt agggggaatt ttataatgaa
    20821 tataaataat gttgtggtaa gaatattagc agagagaatt ttaggtggag gattaaaccc
    20881 tctaaaaaat cgagaatttg aattagatga tgtaactaat gtagagtaca gaaaagctgt
    20941 agaggattat ataattagag aaagtggagt agtagaagga gcagaaccaa ctatatagag
    21001 ggttcttttt ttattgaaag aaggtgatta aatgactttt aaggagttag ttaataaagt
    21061 tagaaatctt gtattagaag caaagaatgt aactatagaa gatacagaga ataattttac
    21121 aagtgataat gtagaaggag cattgaaaga atgtatagat agagcagatt tggcttttca
    21181 aagtgccgat agtggaaaag agctaattgc aactgctatc ggctcaccag ctacaaaaga
    21241 caatactttc gcagaattgg caagtcttat tggggataga aagaagttta aaatgggaaa
    21301 tatacaatta actatggagc agaaaaaggc taatttagaa atagattttg aacccgaaat
    21361 cctaattgca tttattggag aaattgatta ttctaggtat aacacagcaa catattataa
    21421 agaaggcgtt ttattgttag aaggtagaat aaattctata tcatttaaaa ttttaaatga
    21481 aaattcaatt tacagtatag taatgaatcc accatacaat gtatcaacag caccacttgg
    21541 agttacatgg tatgcaataa aataaaacat aaagaaatag taggagagga ctatatagcg
    21601 tagttctttt ttaattcaaa aataaggagg tttacatgaa tgaagaactt ttcgaagcag
    21661 atttaaaaag acatgaaaca agaataaata agcatggaga agaaatagac gaattaaaga
    21721 tagcaaatat agagtctaaa gcagagttaa aagcattgtg tgagaactta aactcactta
    21781 caagtatgct aaagtggcta attggaacta tgattacaac cttagtaggg ttctttatat
    21841 ttgccataca gaaaggaata ttttaattaa ttaggaggat aaaagatgga taatttaata
    21901 agttttatac cagagcagtt actaatttta gttgctgctc tttatgttat aggagcaggt
    21961 tgcaagaaat ataagcaatt agataacaag tatattccag ttatattatt aacacttggt
    22021 gtaggtttct cggtgtggat gttaggatta aatcctagtg cagtcttaca aggagtgatt
    22081 tgttggggta tatcaatagg tataaatcaa acttacaaac agttgaagga ggaaaataaa
    22141 taatgaaaat atgtattaca gtagggcata gtattttaaa aagtggagca tgtacttctg
    22201 ctgatggagt agttaacgag tatcaatata acaaatctct tgcaccagta ttagcagatg
    22261 tgtttaggaa agaaggacat aaggtagata taataatatg ccccgaaaag cagtttaaaa
    22321 ctaagagtga agaaaagact tacaaaatac caaaggttaa tagtggaggc tatgacttac
    22381 tcatagaact acatttaaat gcaagtgatg ggcaaggaaa aggctcagag gtgctatatt
    22441 atagtaataa gggtttagag tatgcaacta gaatatgtaa gaaactaggc acagtattta
    22501 aaaatagagg tgctaaacta gataaaggat tatatatttt aaatagttcc aaacctacag
    22561 cagtactaat tgaaagtttc ttttgtgaca ataaagaaga ttatgagaaa gctaagaaac
    22621 taggtcatga aggtattgct aagttaattg tagaaggtgt attaaataaa aatataaata
    22681 gtgagggagt taaacagatg tacaaacata caattgttta tgatggagaa gttgacaaaa
    22741 tccctgcaac tgtagttggt tggggttata atgatgggaa aatactgata tgtgatataa
    22801 aagattacgt accaggtcag acgcaaaatc tttatgtcat tggtggtgca gcatgtgaga
    22861 agatagggac tattactaaa gaacactata caatgataaa aggtaatgat agatttgata
    22921 ctctttataa ggcattggat tttattgata gatagattag aaggtagcag ctagggttag
    22981 ttgctacctt ctttttttgt actttctttc tttaagtttt ttactttcca tattatctaa
    23041 tacttgtagc gaactcatat agaatttttg gcgactatcc catttttgct gagattcaca
    23101 tattaaacat aaataagtac caaaaaaaac tcctattaca gaacaagcag taaaaaaatc
    23161 ttttataata ttgttagtaa gtagactaaa gaaaattgat ataaatgcag caataatagc
    23221 aattgcaaat ggaattgatg gattgttttt agaatctaag acagttttga tgcgtgtttt
    23281 ttctgttttt atattttcta ttgaatccat ctctttaaga gaattataaa atctgtaatt
    23341 gcatctataa tattcctcag cttcttttat atctggcttt tcttcttctt gaataacata
    23401 taactgttct atagggcttt ttttattaaa tttaataata tcaaatatat catttagaaa
    23461 catattttat acctccat
//
LOCUS       DAEMAE010000043        22597 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEMAE010000043 DAEMAE010000000
VERSION     DAEMAE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.044"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            128..1168
                     /locus_tag="KPH93_001353"
     CDS             128..1168
                     /locus_tag="KPH93_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688182.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFARIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1524..2846
                     /locus_tag="KPH93_001354"
     CDS             1524..2846
                     /locus_tag="KPH93_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG1688183.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3144..4505
                     /locus_tag="KPH93_001355"
     CDS             3144..4505
                     /locus_tag="KPH93_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG1688184.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLILG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4578..5972
                     /locus_tag="KPH93_001356"
     CDS             4578..5972
                     /locus_tag="KPH93_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1688185.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLNAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNIILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6034..6504
                     /locus_tag="KPH93_001357"
     CDS             6034..6504
                     /locus_tag="KPH93_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG1688186.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLARNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6507..8723
                     /locus_tag="KPH93_001358"
     CDS             6507..8723
                     /locus_tag="KPH93_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1688187.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKIFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLEISKEDLYLDNGYVISKL
                     NENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8806..9477
                     /locus_tag="KPH93_001359"
     CDS             8806..9477
                     /locus_tag="KPH93_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1688188.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9840..11054
                     /gene="dpaL"
                     /locus_tag="KPH93_001360"
     CDS             9840..11054
                     /gene="dpaL"
                     /locus_tag="KPH93_001360"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG1688189.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWHGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11154..12341
                     /locus_tag="KPH93_001361"
     CDS             11154..12341
                     /locus_tag="KPH93_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG1688190.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            12474..13844
                     /gene="hydA"
                     /locus_tag="KPH93_001362"
     CDS             12474..13844
                     /gene="hydA"
                     /locus_tag="KPH93_001362"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG1688191.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGAIYRVLKKARDLGCIVEFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCKESLETVKTAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLASGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNVNKEETISYKTQKQNVDYTPYEGFKIKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            13862..15268
                     /locus_tag="KPH93_001363"
     CDS             13862..15268
                     /locus_tag="KPH93_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1688192.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHTHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VAKEYDNALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELAKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTTNEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            15340..15786
                     /locus_tag="KPH93_001364"
     CDS             15340..15786
                     /locus_tag="KPH93_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG1688193.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            15825..16619
                     /locus_tag="KPH93_001365"
     CDS             15825..16619
                     /locus_tag="KPH93_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG1688194.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            16609..18900
                     /locus_tag="KPH93_001366"
     CDS             16609..18900
                     /locus_tag="KPH93_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1688195.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDIVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSRALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLNLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            18901..20214
                     /locus_tag="KPH93_001367"
     CDS             18901..20214
                     /locus_tag="KPH93_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1688196.1"
                     /translation="MIAIKSKYLISSADEIYTNYAVVVENDIIKDILPNEEVEIKYKN
                     TEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAVLSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDKFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATENGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            20250..21335
                     /locus_tag="KPH93_001368"
     CDS             20250..21335
                     /locus_tag="KPH93_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG1688197.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTTNALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKKRVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            21562..22569
                     /locus_tag="KPH93_001369"
     CDS             21562..22569
                     /locus_tag="KPH93_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688198.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEVTVMKGDISEVKQEMTVMKGDMSEVKQEINVLKGDMSEVKQEINVLKGDMFEVKQ
                     EMTVMKEDMSEVKQEMTVMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEI
                     NIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKNEVSTANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVRLKSV
                     K"
BASE COUNT         8796 a         2515 c         4589 g         6697 t
ORIGIN      
        1 tactttttat taagttttgt tttaaaataa tattattaaa ttataactaa gcttttctgt
       61 tatgatataa ttaaatagat aatgtgaatg attattatat agaatactta tttggagttg
      121 atattttatg gacaaacaca gtgattcagt aatgtatggt ggaatggaag tatataatat
      181 ttggggaaca acaacagtag actccataat tggagagtct gacgtaatgc gtgctcttaa
      241 gaatagaatt tgcaaaattg gaaatagtga ttctactgta gctataactg gagaaagtgg
      301 cactggaaaa gaattaatag caagagccat tcatctcgca ggaaacagag ggaataaaac
      361 attcgttgct ataaactgtg ctgctatacc agaaccttta ctagaaagtg aactttttgg
      421 atatacaaaa ggagctttta gtggagcaga ttcaaaaggt aaaattggta agtttgaatt
      481 ggccaatgga ggggttgtat ttttagatga gataggagat atgccaattc aactacaatc
      541 aaagttattg agggtattac aggaaaagaa gtttgcgaga ataggttcaa atgaactaat
      601 tgatatagat gttagaatca tatcagctac taatagagat ttatttgagt tggttaagga
      661 aaataaattt agagaagact tatactatag attgaatgta atacctttag aagtgcctcc
      721 acttagagaa agaggagacg atatacaaat attaataaac aattttatag acaaatattc
      781 gagaaagatg aagaaaaacg tgtaccatat agaaccagat gtggaacaaa tgttgcttaa
      841 atatccttgg ccaggaaata ttagagagtt ggaaaatact atagaactgg cattaaacct
      901 tcttgaggaa gatggaataa tacataaagg cataataaat aggaagatac ttgaatattt
      961 taagttaaat aatataaata taaagttact tgaggaaagt gactatgaga taagtatgga
     1021 acaagacata cttacacttg aagaatttga aagagcctat ataaagaaag tgttaaagtt
     1081 ttacggaaat gatactaagt ctaaaaaact ggctgccaaa aaacttggaa tatcactagc
     1141 tacattgtac agaaaaatag atgtataata attagacttt ttattctcaa ttctaaaatt
     1201 tgaaattata aaattgataa aaaacacttg gaaaactatt ttctaagtgt ttttttgtgt
     1261 aatagcattt tattatccaa aaataaaaaa aatttaaaag aatttaaaaa aataaatgct
     1321 ttaataaatt atcaaatact aacaaatata ataaaaatta tatatgtaaa acaattatgt
     1381 taaaaaattg gcattataat tgcttcatat taagttgaga tgacaaaata aaaaaagaaa
     1441 ggatgttgtg tgagataata ataactgtca ttaaaaaata gttgggagtg ataaccttga
     1501 gtaaatatca aaagaatgtt tctatgtttg atattgatgg aaggccacca attttaaagt
     1561 gtttgccact atcaatgcaa catatactag caatgatagt tggaactgtt acagtaccta
     1621 tagtcgtagg aggagctgtg ggtgttagcc ctacacaaat aacactttta gtgcaatatg
     1681 cattaataat agctggtata tcaacactta tacaactata tccaatagga aatattggtt
     1741 caagattgcc tataattttt ggagttggtt ttacttatgt accaacactt gtagcagtcg
     1801 gaggaaatta tggattagca agcattttgg gtgcacaatt aataggggga gtggctacag
     1861 tattaatagg gatattcata aaaaagataa gaaaattttt tccgaatata gttgcaggaa
     1921 cagtggtttt tacaattgga ttatctttgt atcctatagc aataaattat atggcaggtg
     1981 gtgtaaatag tcctacttat gcttctatgc aaaactgggc agtagcggct ataacactag
     2041 tggtagttat aggtcttagt caatttgcaa aaggatatgc aaaattggca gcaatatttt
     2101 tgggaattat agttggatat tgtgtatctt tagttttggg tataataaat tttgacccta
     2161 ttagagaggc aacttggttt gcgataccta aaccgcttga atttgggatg gaatttgata
     2221 tgcctgtaat aatctcagta gtaattgtaa gtataatcaa cgctgttcaa gcaattggag
     2281 atttatcagc tactacaatg ggaggtatgg atagaaatat aacagataag gaattatcaa
     2341 gtggagtaat aggaagtgga atttgtacta tgataggagc tttgtttgga ggtttaccac
     2401 catcatcata tagccaaaac gtggggatgg tagctatgaa caaagtaata agtagatttg
     2461 tgcttggaat agctggtata tttatgttat tgtcggggtt tgtacctaaa tttggtgcat
     2521 taatgactac tataccatat tctgttttag gaggagctac aatatctata tttagtatga
     2581 taacaatgac aggtataaag ttaattatta cagaagagtt gtcatcaaga aatattacca
     2641 ttgttggttt ggcaattgcc ttaggcatgg gaataacatc ggtcccagca gcccaagagg
     2701 catttccttc atttgtaaag ttagtatttg gagaatctcc aatagtaatt gcaacattgg
     2761 ttgcattcgt tttaaatcta ataataccaa ataaaagtct agaggatgaa gctaaagaaa
     2821 gagctgttat ggaagagact aattaagata taataatttg aaagaattgt ttaaattatt
     2881 taggatgaga ttgttagatt atttttgata gctattcatt ttaataaaag atttaacaaa
     2941 taaatatata tcaggcaatt aatgttcatt taagttgaaa atcgggggag ggaatattta
     3001 gaaatttgtt aattttttat aaaaatagct cagtttagct gtgtttatcg gttgagcaat
     3061 aaattcatgg tcataaaaat atttatagaa aataattata aaaatattta gtcaagatat
     3121 ttatgtatgg gaggatttat tttatgaaaa cacgtaatac ttcaaaatat gatgtcgatg
     3181 ggataccacc actaaaggaa tcaatacctt tggccttaca acatctgtta gcaatgatag
     3241 taggaaatat ggtacctgcg atattaatag caaacgtggt aggacttaac caggggcaag
     3301 caacaatgtt gattcagggt tctatgttag cagcaggttt agcaacgttt ttacagttat
     3361 atccaatacc tttatttaaa ggatttaagt taggttcaag attacctgta atgatgggaa
     3421 tgagttatgt atttttagga gcttgtcttt cagtagcagc taaagatggt ttagcagctc
     3481 tatttggggc acagattgct gctggggtaa tcgtattctt tgtaggattt ggagttaaga
     3541 aaattagaca tatattcact ccaatagttt ctggaactat aatagcatgt atgggacttg
     3601 gattattcgc aacagctata aagaatttag ctggaggaga aggtacacaa acatttggaa
     3661 gcccaataaa cttcacagta ggagttatag ttgcttttgt aataataatg ataaataaat
     3721 acggtaaagg tttagtaaaa aattcttcaa tattaatagg aatattagta ggttatgcta
     3781 tatctttaat tttaggattg gttgatttct cagccgttca aggagcagct atagtatctt
     3841 taccaacacc agcagctttt ggattggaat ttagaccaga gttaatagta atgtttacta
     3901 ttatttacat aataggtata gctgatatga tgggtgcttg tacaatagct acagtaggag
     3961 caatggatag agaagtaact gacgaagaat tagcatcagt tgttttagga aattctataa
     4021 cttctataat ttcatcacta tttgcagctc ttccaactgg agtatttagc caaaatacag
     4081 taattgtatc tatgaacaaa gttacaagta gatttgtaat agcactaggg gcgcttgtat
     4141 tattactagc agggatatct ccagcattgg gtgcaataat gacaactata ccatcttgtg
     4201 ttgttggtgg agcaacttta gtagttttct catcaatagc aatgtctggt ttctctatta
     4261 tgagtatgga tggattcact gaagaaaaca atcttatagc aggtgtatct atagcaacga
     4321 gtatgggtct tacaactgct ccacaagttt tagaccaatt cccagaaaca attggaactg
     4381 tccttggagg atcaagcata gtttctggag caataatagc tatattactt caaacattat
     4441 ttaaattaaa gtcacgtaaa agtgctgaaa acgttacttc aaatcttgag gaaaatattg
     4501 gataaatgta ttagatataa tatgagaaaa ccatatggta aaatgttgta atgaaagtat
     4561 tcaaaggggg atttaagatg attgatatac taataaaaaa tgcaataatt gttacagtta
     4621 ataaagacag agatgttata tttgatggag caatagctat tcaaggtgac agaatcttgg
     4681 acataggaaa tacagttgat attgagccta agtatcttaa tgctaaaaaa gttatagacg
     4741 ctaatggaaa agtaatattt cctggcttta taaatactca taatcacctg ttccaagtgt
     4801 tattaaaagg tttaggtgat gatatggcac ttcatgagtg gttaaataca atgatgtttc
     4861 catcagcaaa attcttaaca gaacaagata cttatgatgc tgcaatgctt ggatgtatgg
     4921 aaggtttaaa aagtggtata actacaatgg ttgattatat gcatactcac aatagaccag
     4981 gacttacaga tggaatagta aaggcatata aagatttagg tataagaggg gttgtaggta
     5041 gaggttgtat agatttaggt attcacaaag agcttataga agatgtggaa acggttgaaa
     5101 aagatttgag aagggtattc gaaaaatatc ataattcaga aaatggaaga ataaaaatct
     5161 gtgtagctcc ttcttcaatg tgggctatat ctgaagaaat gggtaagatg ctttggaata
     5221 tagttaaaga atatgattca tattttacag ttcatatatc tgaaacagaa tttgcaagaa
     5281 cagcaacaaa ggatatacat ggagaaatag atataaaatt acttgaaaaa tggggtatag
     5341 taggtccaga agttgtagca gtacactgtg tgtgcataac tgatgaagac atggaaatgc
     5401 ttaaaaagta tgatataaaa gtttctcata atgtagcaag taatatgtat cttgcttctg
     5461 gtgttgcacc tgttccagaa atgttgaaaa aaggtataaa tgtaagctta ggtcttgatg
     5521 gggcagcaag taataatgca caagatatgg tagagcttat gaaactaaca gctttacaac
     5581 ataaagtaaa taattgtgac cctcttgcaa tttctgcaga gaaagttctt gaaatggcta
     5641 caatcgaggg agcaagaact cttagaatgg aagatgaaat aggttctttg gagattggaa
     5701 aaaaagctga cttggtaata tttaactcaa tgctttctcc aaaatcaata cctcttcaca
     5761 atcctgtatc aactttggta tactcagcta gtatgcacaa tgtagagagt gttctagttg
     5821 atggaaatat aatacttgaa aatggaaaag taactacaat tgaaagtgaa gaacaagtgt
     5881 tacttaatgc acaacatgca gcagaagaat tatgtaagag agctaatgta actaatagaa
     5941 tggaaggaca taagtggaat agcttatact aatattaaaa taagattgta ataatattct
     6001 attacaacat tagtttatct gtaggaggag aaaatggaaa agataaaatt aaatattaat
     6061 gattacatgt atgaaataga agttgaaaag aatgaaactc ttttgcatgt acttcgtgaa
     6121 aaattaaatc taactggagc gaaacaaggt tgtaatagtg gcagttgtgg agcatgtaaa
     6181 gttttgatag atggagttga catgaaatct tgtaaacttc ttgcaagaaa ctgcgaaggt
     6241 aaagttatat acactataga aagttttgct aatggagaaa atcttcatcc aatacaagaa
     6301 gcttttatag aagttggagc tgtacaatgt gggtattgta ctcctggcat gattataaca
     6361 acacaagctc ttttaaatag aaatgcatct cctaatgaag atgaaattag agaagctttt
     6421 aaagaaaatc tctgtagatg cacaggatat gtaaagatta tagaagctgt aaaattatct
     6481 gcagctagat tagggggtag ataattatgg aaaaggtaat aggaaaatca taccctctaa
     6541 aagatgcagc tttaaaagtt actggtcaaa tgaaatacgt ggctgatatg aaaccacaaa
     6601 atgtacttta cgctaaaatg cttttaagtc ctgtagcaca tgcaaaaata aaaagtatag
     6661 atacgagtga agctgagaaa gtagtaggtg ttagagctat agctacttat ctcaatacat
     6721 cacaaagaaa atacaatggt tcattaagat tttatgagca caatatacct gaaaatgaag
     6781 ttatattttc agatacagtt agatttgttg gtgaccgtgt tgcagcagta gcagcagaaa
     6841 ctccagaaat agcagagaaa gctgtaaaac ttataaaagt tgaatatgaa gaattgccag
     6901 ctgtatttac tatacaagat gcaataaaag aagacgctat ctgcatacat ggagattcaa
     6961 atatagtttc tcaaaacaac ataaatgggg gagatgtaga aaaaggcttc aaggaatgtg
     7021 actatatatt tgaagatgaa tactctactc cagcaataca tcatagtgca atagagactc
     7081 atagtgtaat tgcacactat gattataatg ataaattaac tgtacataca ccttgccaaa
     7141 atacatttgg atttagaata atactgtctc aaatttttgg gatgtcatta aataaagtaa
     7201 gagttataag acctgctatt ggaggttcat ttggtggaaa attagaactt acaattgaac
     7261 cagtagcagc cgcattatca aaaatgacaa gaagaccagt taaaatggtt cttacaagaa
     7321 aggaatctat gatatcaaca agaactcgtc atgggtcttt tgtaaaatta aaaactggtg
     7381 taaataaaga tggtgaagta atagcacaag atataaaaat atatacaaat acaggagcat
     7441 atgcatcaag tgctttaaat gttataggag cacttagtca taaagtattt aaagtttata
     7501 aaataccaaa tataaagttt actgggatgc ctgtatatac gaatacacca atagcaggag
     7561 ctatgagagg atatgggtca cctcaaatat ttatggctca acaggctcaa tttgcgaaaa
     7621 tagccaagga aataggtatg gatttagtcg attttcaaaa taaaaatgca gtagagccag
     7681 atgatgtcga tataatcttc cacggttctc ttggaaatcc tagagtattg gattgtatag
     7741 agcaaggtaa gaagatattt aagtgggatg aaaagaaaaa acttccaaaa gaagaaggaa
     7801 aatatttaag aggtataggt atggcaatag gtgcacatgg taatggagtt tttggtgcac
     7861 atagagatgt tgttacattg acacttaaat taaatgaaga tggtacattg acactactaa
     7921 caggagcaca tgatatgggt aatggtgttg taactatgca aacaatgatg gtagctgaag
     7981 ttttaggaat aacacctgat aaagttgata catttgaaac tgatacagat gcatgtacat
     8041 tcaaccttgg tgactatgca agtagagggg tatttgtaga aggtggagga gctaaaaaga
     8101 cagcagagaa attgaaaaac cttatattag aagaagcaga aaaattactt gaaatatcaa
     8161 aagaagattt atatttagac aatggatatg taattagtaa acttaatgaa aatgtaaaag
     8221 catcactttc tgatgtcgca gtatatagtc aaagtaaatc attaagagag ttaactgttg
     8281 ttgaagatta ttcttcacca gctggacgta cttcttatgg tgtacatttt gctgaagttt
     8341 tagtagataa agaaactaag gatataaaag tagttgattt tgtagctgta catgatgttg
     8401 gtagagttat aaaccctcta aatttagaag gacaattaga aggtgggatt catatgggtc
     8461 taggatacgc attgagtgaa gagttggcat ttgatgaaca aggtagacct aaagctactg
     8521 attttaaaac atataaatta tttagaactg tagatatgcc aaagtgtcaa gttgcatttg
     8581 taaatggtta tgaaaaagct ggcccttttg gtggtaagag tattggagaa tgtgctgttg
     8641 taccagttgc accagctgtt gcaaatgcag tatataatgc aactgatgta gaaatacatt
     8701 cattgccaat aaaattaaaa tagtagaaat taaagcacaa ataacaattt attgtatttg
     8761 taaatagata ataaaaatag atttatttgg tgaggagata ttattatggc aaagatatta
     8821 ggtatttgtg gaagtccacg taaaggagca acagaatatg cagtgttaga agctttgaaa
     8881 gaagcagaaa ctatacctgg tatagaaact gagtattggt cagttagagg gaaaaaaata
     8941 agtccttgtg tacattgtga tgcatgtata agaaaagaaa ctatgtgtat tataaaagat
     9001 gatatccaag aattagagca aaaaatacta gaggccgatg gaattatagt aggttctcca
     9061 gtttatgata tgaatataac tgctcaactt acagcagtat ttaatagact tcgtccaatg
     9121 tatttagttc atccaggaaa acttcaaaat aaggttggtg cagctataac tactggtgga
     9181 actagatatg gtggccaaga attgacaaaa cttcctataa taaatttctt cctaatgcat
     9241 gaaatgttag tatctggagg tttaggtgga tgctatatag gtggtacaat atggagtaaa
     9301 gatggaaaag caaaaggtgc ccaagaggat gagacaggta tggacacagt aaaaagactt
     9361 ggaaaaggtg cagcagaggc agttatggtt tctaagtttg gtcttgaaaa atggaatgtc
     9421 gtaaaagaag aattagatgt taaaaaagat gaaaaatcac ctcttagaga ccactaaaga
     9481 tttattatat tattgaatat atacaaatag tgatgtaata gattattgca tcactatttt
     9541 atgttttaat ttagatttta taaattttat attaaaaaat atagaaacaa agtgttttga
     9601 gatttatttt taaattttaa aaatgagaat taaaaagtga gaagatagaa aaggtttttt
     9661 agttattaaa ttctacatac attgtatgaa ctaaagatag aaaagaaaaa ataataaaag
     9721 cattgcaatt tctatactaa agagtcatga aaaaatatat agtatatatt tagtgttggc
     9781 atgttaattg cttatatatt aaagtataaa aatttaacaa taatttggag gtattttaga
     9841 tgttaaaaga aattaaatgg aaagtaaata atttgccaaa gggcgataag gaaaattgta
     9901 tcaaattttt aaatgaagaa gaaataacta aggttagaaa ttttcacaaa agctttcctc
     9961 aatataaaga aactccatta gcaaacctag aaggtttagc aaaaaaatta ggtgttgcag
    10021 gagtatatgt aaaagatgaa tcttatagat ttggattaaa tgcatttaaa gtattaggtg
    10081 gttcttattc tatgggtaga tatttagctc aaagattaga tacagatata tctgagttag
    10141 ggtatgacaa attaacttca aaagaaataa aagaaaaatt aggagaaata actttcttta
    10201 cagccacaga tggaaatcac ggtagaggag ttgcttggac agctaataaa ttaggtcaaa
    10261 aatctgttgt attaatgcca aaaggttctt ctgaatttag attaaataaa attaaaggag
    10321 aaggtgcaga tgctagtata acagacttaa actatgatga tgcagtaaga ttagctaatg
    10381 attatgcaga agctgatgac catggagtaa tggttcaaga tacagcatgg cacggatatg
    10441 aagaaatacc agcgtggata atgcaaggat atggaactat ggctcaagaa gctatagagc
    10501 aattaaaaga atatggagta gatagaccaa cacacgtatt tgttcaagca ggagttggtt
    10561 cacttgctgg tgcagttcaa gggtatgttg cttcgatata tgatgaatgt ccaataacag
    10621 tagtagtaga ggctgatgaa gctgattgtt actataaatc agcagaagct ggagatggaa
    10681 aaccaagatt tgttggagga gatatgccaa ctataatggc agggcttgct tgtggagagc
    10741 caaatactat aggatttgaa gtattaaaaa atcatgcagc agcatttgtt tcagcaccag
    10801 actgggtatc tgctaaaggt atgagaacat taggtaatcc tttaaatgga gatgaaaagg
    10861 tgatttctgg agagtctgga gcagttacaa ctggtctttt agtagcagct atggaaagag
    10921 aagatttagc agatttaaga aaagatttaa aattagatga aaattcaaga atacttttaa
    10981 taagcacaga aggagatact gaccctgaca aatatagaag tatagtttgg gatggagaat
    11041 atccaagtat ataaggaatt actaaaatgc aattattaga tactggttta catatttaga
    11101 aaatatatag tatataaaat acatacatta taaaaatcta gggggataat gaaatgttaa
    11161 atgaacaaag aaaacaagaa gtaactgaaa tatgccaaaa attaattcaa aatcctagtt
    11221 cttctggaaa tgaagaaggt gttgtaaaag caatagaaga gtcttttgaa aaattaggtt
    11281 ttgatagttg gtcaagagat agatatggaa atatagtagg atgtataaaa ggtaacaagc
    11341 caggtaagaa aatattattt gatggacata tagatacagt tccagttcca gatgcaagta
    11401 aatggagtgt tcctcctttt gaaggaaaaa tagttgatgg aaagatatat ggtagaggaa
    11461 cttctgacat gaaaggtcaa tatactgcaa tgatgtctgc tgtagcgtat tttgctaaag
    11521 acactaatag agattttgct ggagaactat atgtagcagg tgttgttcat gaagaaatat
    11581 ttgaaggagt ttcagctaga gagataagta aagctgttca acctgactat gttgtaatag
    11641 gtgagtcttc agagttaaac ttaaagattg gtcagagagg tagaggagaa atagtagtag
    11701 aaacttttgg taaaccagct cactctgcta atccagaaaa aggtgtaaat gcagtttata
    11761 aaatggctaa tgtaatccaa aagatacaac aattagtacc tccaactcat ccagttttag
    11821 gtgatggaat attagtttta acagatataa aatcttctcc atatccagga gcatcagtag
    11881 tgccagatta ttgtaaagct acttttgaca gaagattatt agttggagaa acaagagaag
    11941 gcgtattagc tccaatacaa gctttattag atgaaatgat gaaagaagac ccagaattaa
    12001 atgcaaaagt ttcttacgca gttgaaaaag ctgattgcta tacaggaaat actatagaaa
    12061 gtgaaagatt cttcccaggt tggttatatg acgaagaaga tgaatttgtt caagcagcat
    12121 ataagggatt aaaagaagct ggaatagatt cagaaataac tcaatactca ttctgtacaa
    12181 atggaagtca ctatgcaggt gaagcaggaa taaaaactat aggatttggt ccttctaagg
    12241 aaaatcttgc tcacacaata gatgagtata tagagcaaga acagttattt ataggaacag
    12301 aaggttacta tggaatatta aaatctgtat acggaaaata atatttagtt cacaataaat
    12361 tatttaaata tagtctaaag gtgctgaata tagcaccttt agaaaataca attttaaata
    12421 aagttttacg atacttggaa aatactttaa agcttaaaag ttaggaggta tatatgggaa
    12481 caatactaag aggtggaact ataattacct ctgataagac ttatataagt gatttaagga
    12541 tagaagatga aaagatagtt gaaataggaa ataacttaga aataaatggt gataaggtta
    12601 ttgatgctac aggtaaaatt gtaattcctg gtggaataga cacacatact cattttgata
    12661 tgaatgcagg ttctattaca actgcagata attttgaaac aggaacaaga gcagctatag
    12721 ctggaggaac tactacaata ctagattttg cagaagccaa tgagggggaa aacctactgc
    12781 aaggtgtaga agcatatcat aaaaaagcaa gtggaaattg ttattgtgat tatggttttc
    12841 atatgacaat tacatgttta aataaagata catttaatca tatggaaaaa cttatttcag
    12901 atgggatagt atcttttaag atgtatatgg catatgatgg catgaaagta gatgatggag
    12961 caatatacag ggttttaaag aaagcaagag atttaggttg tatagttgaa tttcattgtg
    13021 aaaatgggga tttactagat gtattgatag aagaaaatat acaaaatggt aatttagagc
    13081 caaaatatca tccacttaca agacctaaca tagttgagaa agagtctgtt tcaagacttg
    13141 ctgatataac taaattatct aattcaaaat cctatgtagt ccacttaagt tgtaaggagt
    13201 cacttgaaac tgtaaaaaca gctagagaaa aaaatataga tatgatagta gaaacatgtc
    13261 cacagtatct attattagaa gacaatctat ataataagga taaatttgag ggtgctaagt
    13321 atgtaatgtc tcctccttta agaaaaaaag aagacataaa ttacctttgg gaaggattgg
    13381 ctagtggaga tattcaaaca gttggcacag accactgttc atttaacttt aaggggcaaa
    13441 aagacttggg aattgatgat tttagtaaga taccaaatgg agcacctggt gttgaacata
    13501 ggctagccct tttatataca tatggggtct tagaaaatag aataagtgca aataagtttg
    13561 tggaagttac ttctacgaat gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga
    13621 tagctgtagg aagtgatgca gatatagtca ttttaaatgt taataaagaa gaaacaatta
    13681 gttataaaac tcaaaaacaa aatgtagatt atactccata tgaaggattt aagattaaat
    13741 gtaaagttga agatgtattt ttgagaggaa atcatgtagt acaaagttgt aatgttaaag
    13801 aacatccaac aggacaatat attaaaagaa aaattactaa ataatgcggg gggaaataag
    13861 aatgatggat atacttatta agaatgccat aatagtaact gtgaataaag agagggaagt
    13921 tatttttgat ggggcattag tagtaaaaga taacaagatt gctgatattg gaaattctaa
    13981 agagatagaa agtaaatata ctgatgttaa aaaaattata gatgctaaag gtaaagtttt
    14041 attcccaggt tttataaata cacatactca tttattccaa actttattaa aaggattggg
    14101 agatgatatg gtgcttaaag actggttaga aactatgacg tttccagccg ctaactattt
    14161 agagccaaaa gatacttatg atgctgcaat gcttggatgt attgaaggtc ttagaagtgg
    14221 tataacaaca atggttgact acatgtatcc tcatagtaag ccaggtcttt gtgatggaat
    14281 aatagatgct tataaggaat taggaataag aggaatactt ggaagaggat gtatgaatac
    14341 aggagctcaa tttggagttc atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga
    14401 tgtaagaaga ttgtttgaaa aacaccataa tacagaaaat ggaagaatca aaataggagt
    14461 agctcctgca gcaatatggt caaattctca agaaatgctt gaaatgttgt ggagagtagc
    14521 aaaagaatat gataatgctt tatttacagt acatatatca gaaactcctt ttgatagaga
    14581 agcagcaaaa gaattgcatg ggcaatatga catagatgtt ttggagaaat taggaatact
    14641 tggaccaaat gtgcttatgg tacattgtgt gtatctaact gaaaaagata tggaacttgc
    14701 taaaaaatac gacatgaaag tgtctcataa tacagcaagt aatatgtact tatcttcagg
    14761 tgttgcacca gtaccagaaa tgcttaaaaa gggaataaca gttagtttag gagtagatgg
    14821 agccgctagt aataactctc aagatatgct tgaacttatg aaattaacag cacttcaaca
    14881 taaagttaat aagtgtgacc cattagcaat gtcagcagaa aaagttcttg agttggcaac
    14941 tatagatgga gcaagagcta ttggaatgga agatgagata ggttcattgg agataggtaa
    15001 aaaagcagac ttactaatat ttaatccaat gctttcacca aaagcgatac ctatgcataa
    15061 tccagtatct acattagtat attcttctag tatgaaaaat atagagagtg ttatagtaga
    15121 tggaaatata ataatggaag attcaaaaat attgacaact aatgaagaga aagcattaaa
    15181 agatgctcaa gatacagctg agagactttg tgtaagagga actataaaaa atagaatgga
    15241 aggtcataaa tggaatagcc tttattaata aatgtaattt attcataaaa aagtatagta
    15301 aaactttagc ttaaaaatat gagttttgag gtgatttatt tgattatcaa atgtaccata
    15361 aataataaag aaaaagaatt aagtataaga ccagatgatt ttttagcaga tacattaaga
    15421 gaaaatggat atgtaagtgt taaaaaaggg tgtgacacag gctcatgtgg cttatgtaca
    15481 gtgatggtaa atgacaaggc tgtgctttca tgtaattatc ttactgctag agtagatggt
    15541 cagcatatta caacacttga gggtgtcttg gatgaagcaa agaagtttgg agattttctt
    15601 gcagacgaag gggctgacca gtgtggattt tgtgctcctg gatttataat gatggtcttg
    15661 gcaatgaaaa gagagcttca aaatccaaca gaagaagaag ttatagatta tgtaaatggg
    15721 aacctttgta gatgtacagg atataaaggt caattaagag caatacataa atatttaaac
    15781 acttagactt ttaagtgaaa attaagttga tatggggaga gagtatgata actataaagg
    15841 aatatgtagt tccaaaaagt ttagatgaag cttatgaact gctaatatca agaaaaaaca
    15901 acataatact tggaggatgt ggatttttaa aactaggtag taaaaatatt ggttcagcaa
    15961 tagatttaaa agacttagct ttagattata taaatgaaac tgatgatagc atattaattg
    16021 gagcagacac aagtttaaga actttagaat taaataaggt tattaaaaat tactgtaatg
    16081 gagtaatttc aaacgctgtt tctaatatag taggagttca atttagaagt ggagctagag
    16141 taggagcaag cgtatttgct aagtatggat tttcagattt aataccttca ttactagtgg
    16201 tagatgcaaa ggttaagtta tataaaaaag gggttatgaa cttagctgat tttttagaaa
    16261 atgaacttga aaaagatata ttaatagaag ttatattacc taaaaaagat gctataggtg
    16321 tatttgattc tataagaaaa tgtacaggtg attttgcagt attaaatgga gcaatgttaa
    16381 aagaaggaag tatttataaa attgcaatag gtgctagacc aagaagagca aaaattgcct
    16441 ataaagcaag tgaaatatta agtttagaaa atgatataga aaaagctggt gaagtatcaa
    16501 gtgaagaact ttcatttgga agtaatataa gaggaagtaa agaatacaga aaagacatgg
    16561 caaaagcctt agtagtgaga atgtataata gtattggagg tgagtgtgat ggtaaataaa
    16621 gaggtaagaa aaatagatag tgaagccatt gttactggaa aaccaatata tacagaagat
    16681 ttaatttttc ataaagatgt tttaacaatt aaacttctta gaagtccaca tgcttttgcg
    16741 aagataaaaa acatagatat aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc
    16801 acttacaaag atgttccaaa ttatagatat tcaatggttg gggaatcata tccagaagca
    16861 tcacctcatg accaattaat acttgaagat atagttagat atgtaggaga tgaagtcgct
    16921 ataatagccg ctgaagatga aagggctgct attaaagcta tgaaacttat aaaagttgaa
    16981 tatgaagtaa tgactcctat attggatgca agagaatcag aaggaaataa aattttaatc
    17041 catgaagaag atgatttgtt ttgtccattt gattttggat tggatgctac aaaaaatatc
    17101 gtatcgagag aaaaaatggg taaaggggat gtagaaaaag agtttaaaga aagtgatgta
    17161 atagtagaac attcttatat gactcaatca caagcccatt gtatgatgga aactcataga
    17221 gcttatacgt atatagatac gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct
    17281 tatcatatga gaagacaggt ttctagagca ttgggaatac ctctttcaaa agtgagagta
    17341 ataaaaccaa gaataggtgg aggttttggt ggaaaaaaca tagctgtaac ggagatatat
    17401 gcatcatttg taacttggat gacgaaaaga ccagcaaaac taatttatac tagagaagaa
    17461 acttttgcaa tgactaatac tagacatcag atgttttttg atataaaaat tggggcagat
    17521 aaagaaggaa acataaaagc tatagatatg aaagcattga acaatacagg cgcttatggt
    17581 gataatggac cttcagtttg ctctgaatca ggtcataatg tacttccaac atataataat
    17641 gtacctgcaa taaaatttga tgggagaact gtatatacta atttagttcc agggggagca
    17701 ttgagaggat atggagcaac acaaggtaca tttgcacttg attgtgcaat tgatgaatta
    17761 gcgcataaac ttaatatgga cccaacagaa ataaagttaa agaatattat aaaaaaacaa
    17821 actgaaggcg gaataatgaa ttttccaata agaagttgtg cacttgatga atgtataatt
    17881 agaggtaaaa aacttattgg atgggatgaa aaataccctg ttagggatat tggaaacaat
    17941 aaggtgaggg ctgttggaat gtctgtggga actcatggtt ctggtatagc caacatagac
    18001 atggcaatag taaatatgag gatggatgaa gatggaactt ataagttatt ttctgggtct
    18061 tcagacttag gaactggttc tgacacaata cttgcacaaa tagctgcaaa agcattgaat
    18121 acatctattg acagaatttc cgtttataca ggagatacag atatgtgtcc atatgatact
    18181 ggtgcatacg cttcttgtac tacttatgta actggaaatg ctacaataaa atctgctgaa
    18241 agtttaaaac aaaagatatt aaaagtagca cagaagaaac tcaatttacc aatagatgat
    18301 ttaatactat atgaagacag agtttgttat agtgaggatg tagagaagtt tgttttgtta
    18361 agtgaattag gaaaagaatc tgttggtggt ggaaatcaag aagttttata ttcatctgaa
    18421 tcttatggta tacaagagag tgctaaacca ttcttagcag gatttgctga aatagagtta
    18481 gataaaacaa caggggagtt taaagtaata aattatgctt gttctacaga ttgtggtaca
    18541 gttatgaatc ctgcacttgc tagaatacaa gttgaaggtg gtgtaactca aggtataggg
    18601 cttgcaatgt atgaagaccc tagacatggt tctgatggaa agcttcaaac ctctaatcta
    18661 ttacaataca aagttccaac aaaaaaagat ataggaaaca taattgttga ttttgtagat
    18721 aattatgagc caacaggtcc atatggagct aaatctttag gagaaatagt tattcataca
    18781 cctgcaccag caatagcaaa tgcaatatac aatgccacag gagttcgtat aagagaactt
    18841 cctataactt ctgagaaagt ttatatggcc atgaaaaaat taaatcaggg aggtaaataa
    18901 atgattgcta ttaaaagtaa atacttaata tcttctgctg atgaaatata tacaaattat
    18961 gctgttgtag tagaaaatga cattataaaa gatatattgc caaatgaaga agttgaaata
    19021 aaatacaaaa atactgaaga aataatagat aaatcagatg ctataataat gccaggattt
    19081 gtaaatggtc atatgcatca atatggagtt ctttctaggg gaataccagc aaatgtacat
    19141 tttactgact ttgaaggttt cttaaatgac tattggtggc catttataga aaacagaatt
    19201 ggattgaaag aagtaaaggc aacaactaaa gcatcagcaa tagaacttat agaatctggt
    19261 gtggtagctt tttgtgatac cttagaagct ccaaatacag aagaaggaac tttaattgag
    19321 caaggaaaaa tactagaaga gataggtatg aaagcggttt tatcattaga aagttgtgag
    19381 agaataagct ttgaaaatgg gcttagatgt ttagacgaaa attctaattt aataaagtgg
    19441 agtagagaaa atagtaaatt aataaatgga ataatgtgta cacatacaag cttcacatgt
    19501 tcggataaat ttattaagaa agctaaagaa gatgcaaaga aattaaatgc accgatgcaa
    19561 tttcatttat gcgagagtat atatgagcca aactatgctg aaaaacattt tggcaaaaaa
    19621 gcagttgact actataatga tttagatata ttggatgaaa ctgtattagc atctcagtgt
    19681 gtaaaagtaa atgatgaaga aatagatata ttaaaagaaa aaggtgtaaa agtagttcat
    19741 atgcctctta gcaactgtga agtaggtgga ggtttttctc cagtaccaaa aatgattaaa
    19801 aaaggcataa aagtggcact gggaactgac ggatatataa atgatttttt tgaagttatg
    19861 agaggtgcat ttttaatgca taaatcagtt gaagaagatg catcagtaat gccagcaaat
    19921 ttagtattta gaatggcaac agagaatggt gctcatgtat taggacttca aaattctggt
    19981 aaaatagcag ttggtaataa ggctgatata atagttatgg aagatgaatt taaaactcct
    20041 gtgactcttg ataatatatt tgaccagata gtagttcagg gtaaaaaaga atttatatca
    20101 aatgtataca tagatggtag acatatttta aaagaaaaac agttagttga tttagataaa
    20161 aaagctattg ttaaagaaat gaaagaagtt gcttgtgaat tttggaaatt ttaacgaatg
    20221 taaatatata tgaaaagggg aagcagacaa tgtataattt tttaggtaaa gaattaaaat
    20281 caccacttat aataggttca ggaccattaa catatagtgc agctggatgc aagatattaa
    20341 gtgatgcagg agcaggggca gttgtgacta agactataag aaaagaaaga gctataaatc
    20401 cagcaccaca tatggttaga aatacaacta atgctctttt aaataatgag aaatggacag
    20461 atttcgagcc agagcaatgg atagattttg aaataccaca aatgaaaaga gatgggactg
    20521 tctgtatagc ttcagtaggt catacaattg aagaaagtag cgaattagta gagaaagttg
    20581 caaatgcagg agctgacttt attgaattag tatcttatga ctatagagat ttgataccaa
    20641 tgcttaaaga tgcaaagaaa agagtaaata tacctgtaat agttaaactt cctcctatga
    20701 tagatgagat aggcgatttt gctaaaaaat tagaagaagc aggagcagat gcaataactg
    20761 cttgtgattc tgtaggacca gcatttagaa tagacataga aacagggcaa cctttattag
    20821 gtggaaatgg aataggatat ttaagtggag aaactatcaa gccaataact ttacaaagaa
    20881 tatatgaaat aaaaaaacaa gttaatatac caataattgg attaggtgga tgtgtaagtg
    20941 gagatgatgc cttagaaatg attatggcag gggctgactt tgttggtata tgttcagtag
    21001 taatcttaaa aggtgcacaa gtaatatcta aaatacatga tgatttaaaa tctaatctta
    21061 atagattagg atataatact atagaaaatg cttgtggtat agtacataat catatgggag
    21121 atgttaatga aagacttagc tttgagttta cttatgatga ggaaaaatgt actaaatgta
    21181 agatgtgtga gagagtttgt gcatatcaag cgagaaaatt gaatgataag atggaattga
    21241 atgaagaaga gtgtagatat tgtggacttt gtatatcagt ttgtaaacca aaagcacttg
    21301 gaagaaaaat ttcatgttca gagctgaatg tatagattat tttataaaaa ataagtttta
    21361 aataaaaata agaaaggcta tctcaaagag ataagccttt tttgttgttg accttatttt
    21421 tgtttatttg tagttatttt agatattaat tagttttgaa gtaataaagt gaatcaattt
    21481 tgtttaatta aaattttaat aattctgata tatgttaaca ttgtatagta tttgtgtaaa
    21541 atgttataat aattaatagg gatggtgatg caagtgcaag agaagatttt agagttatta
    21601 tatactataa atgaagatgt agttagcata aaaaatgata tgtccgaagt aaaacaagaa
    21661 ataaatgtgt taaaagaaga tatgtccgaa gtaaaacaag aagtgactgt aatgaaagga
    21721 gatatatcag aagtaaaaca agaaatgact gtaatgaaag gagatatgtc cgaagtaaaa
    21781 caggaaataa atgtattaaa aggagatatg tccgaagtaa aacaggaaat aaatgtatta
    21841 aaaggagata tgttcgaagt aaaacaagaa atgactgtaa tgaaagaaga tatgtccgaa
    21901 gtaaaacaag aaatgactgt aatgaaagag gatatgtctg aagtaagaca agaaatgact
    21961 gtaatgaaag aagatatgtc tgaggtaaaa caggagataa atgtattaaa agaagatatg
    22021 tccgaagtaa gacaagagat aaatataatg aaagaagata tgtctgaagt aagacaagaa
    22081 atgactgtaa tgaaagaaga tacatcagag gtaaaacaag agataaatgt aatgaaaaaa
    22141 gacatgtctg aagtgagaca agaaataact gtaatgaaag aagatgtttc cgaagttaaa
    22201 tcagaagtaa actttatgca aaataaaata aataacatta atgaagatat gaatggaatt
    22261 aagaatgagg tatccacagc aaatgaaaaa ttatatggaa ttgaaattaa gattgatagt
    22321 ctagaatgtg aagataaatc aatgaaagag atacagatag aacagaataa tattttagga
    22381 agtttattac ataattctga aataaataaa gctactcatg ataatataga acataatata
    22441 gcatatataa aaggtgatac taattctata aaagaggata tagctgaaat aagaagagac
    22501 ttaaatttag tagagctagc cactagtaaa aattggagtg atattgtcag actaaaatca
    22561 gtaaagtagt attttaattc actaatttat catccac
//
LOCUS       DAEMAE010000044        22333 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEMAE010000044 DAEMAE010000000
VERSION     DAEMAE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.045"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..715
                     /locus_tag="KPH93_001370"
     CDS             170..715
                     /locus_tag="KPH93_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG1688199.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            728..1741
                     /locus_tag="KPH93_001371"
     CDS             728..1741
                     /locus_tag="KPH93_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG1688200.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVSPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYNEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1963..3936
                     /locus_tag="KPH93_001372"
     CDS             1963..3936
                     /locus_tag="KPH93_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG1688201.1"
                     /translation="MSATEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIIKLEEKILTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4005..4364
                     /locus_tag="KPH93_001373"
     CDS             4005..4364
                     /locus_tag="KPH93_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688202.1"
                     /translation="MKLLMILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDLDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4399..4755
                     /locus_tag="KPH93_001374"
     CDS             4399..4755
                     /locus_tag="KPH93_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG1688203.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4792..6084
                     /locus_tag="KPH93_001375"
     CDS             4792..6084
                     /locus_tag="KPH93_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG1688204.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLEHKA
                     TIVPKAGLVAEVMTIAKKDLKKGDFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6520..7158
                     /locus_tag="KPH93_001376"
     CDS             6520..7158
                     /locus_tag="KPH93_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688205.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7165..8007
                     /locus_tag="KPH93_001377"
     CDS             7165..8007
                     /locus_tag="KPH93_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG1688206.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGILQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            8068..10695
                     /locus_tag="KPH93_001378"
     CDS             8068..10695
                     /locus_tag="KPH93_001378"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG1688207.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRI
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGVEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            10909..11154
                     /locus_tag="KPH93_001379"
     CDS             10909..11154
                     /locus_tag="KPH93_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1688208.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKR"
     gene            complement(11443..12741)
                     /locus_tag="KPH93_001380"
     CDS             complement(11443..12741)
                     /locus_tag="KPH93_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG1688209.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGSLTDDEYKVLMDKYKKDYEKYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(12775..15909)
                     /locus_tag="KPH93_001381"
     CDS             complement(12775..15909)
                     /locus_tag="KPH93_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG1688210.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(15913..17136)
                     /locus_tag="KPH93_001382"
     CDS             complement(15913..17136)
                     /locus_tag="KPH93_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG1688211.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            17388..17873
                     /locus_tag="KPH93_001383"
     CDS             17388..17873
                     /locus_tag="KPH93_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1688212.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFELFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            complement(17984..20212)
                     /locus_tag="KPH93_001384"
     CDS             complement(17984..20212)
                     /locus_tag="KPH93_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG1688213.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNA
                     EKEVAISKDSNNKKEVKNDDENPSLGKCPVCQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDALLKYNKNPDTGYFNWKIEF
                     DN"
     gene            20325..20756
                     /gene="dut"
                     /locus_tag="KPH93_001385"
     CDS             20325..20756
                     /gene="dut"
                     /locus_tag="KPH93_001385"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG1688214.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPSGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDISIEEVKELSDSERGKGGFGSTGF"
     gene            21016..22206
                     /locus_tag="KPH93_001386"
     CDS             21016..22206
                     /locus_tag="KPH93_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1688215.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGAESVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSAKKVTISNINSSYYSSRYVNA
                     RRVL"
BASE COUNT         8061 a         2899 c         3755 g         7618 t
ORIGIN      
        1 aaaattgaaa aaagatgtat ttatttatca aaaatattat attaaaatga tattaataaa
       61 aaacatttat aatattagca tatagtatag aaaaaagatg taatttgtaa aatttatggt
      121 aagcagtaaa attaaaaatt taaagatatt atagggaggt tataatttta tggaaaacgt
      181 tattacaggt ctaagtaaag gtgcagagtg gtttatcggt ttgtttcaga aagggggaga
      241 acaatttata ggtcttgttt caggtactct tccaacgcta attgtattaa tggtagctat
      301 taactctttg attaagataa ttggtgagga aagagttaat aattgggctt ctaagctagg
      361 aaagaactct tttacaagat atattctact tccattagta tcagtatttt tcttgggaaa
      421 tcctatgtgc tatacatttg gtagattttt aaaagaagaa cacaaagcag gattttatga
      481 tgcagcagtt tcatttgtac atccaattac aggtcttttt ccacatgcaa atgcaggaga
      541 actttttgta tggttgggaa tatcagcagg tttgactact ttaggcaaag aaactactac
      601 actggcattg tggtatttta tagttggtct aattgtaatt tttataagag gtatagtaac
      661 agaaaaaatg tatgcttttc taacaagaaa aaatagagca acaaataagg cttaggaggt
      721 agataaaatg gaggagaata aaatattaaa aatagaaaag ggaacttcag gctggggagg
      781 tcctttgtat ataaaaaaag aagggaatag aaataagata ctatcaatga ctgctggtgg
      841 tattcatgaa gtaacattaa aaataaaaga actactgggt tgtgaaattg tagatggatt
      901 taaaactggg gtttctgatg aagaagtagc agtagttatt attgattgtg gtggaactgc
      961 tagatgtgga gtttatccaa agaaaaaaat acctacaata aatgtaaatc cagttggaaa
     1021 aacaggtcct ttagcaaaat ttataacaga agaatattat gtatcagatg ttagtcctaa
     1081 ctgtataagt gttgtagatg gggaagatat gccacaaaaa tctcaagaaa ataaaagtga
     1141 aaataagagt tctattagaa agcctgataa ttataatgag gttaagtcaa aagcacaagg
     1201 tgaatatgct aaaaagaaca taatactttc tattggtcaa ggggcagggc aagtagtatc
     1261 aaaattttat gatgcaggcc gagatactat ccaaatggta atgaataacg taataccatt
     1321 tatggcattt gtaagtatgc ttatgggaat tattttagca tctggactgg gagattggat
     1381 tgcaagagtt atatctccac ttgcaggtaa tattggtgga ttattgatta tatcagtaat
     1441 ttgtacactt ccattcctat caccaatact aggacctggt gcagttatag ctcaagtagt
     1501 agggactctt gtaggtacac aaattggtct tggtgcaata ccagcatatt tagcacttcc
     1561 agctctattt gcaataaatg ggcaagctgg atgtgatttt gtaccagtag gattaagttt
     1621 aggagaagca gaaccagaaa ctgtagagta tggtgttcca gctttatttt attctcgttt
     1681 aataactggt ccaatagctg ttattattgc atatggagtt gcagtatttg cattgagata
     1741 atatttaatg tcaatataag gcttggaatt aattaataaa attgtaaata taaatttaag
     1801 ctagtcaaga ttttttgtta atgaaagact agcttttata ctattaatta gctgtaatta
     1861 tcgagttaga ttaatactaa attttccttt attaaagtga aattttatgt attaatttgg
     1921 tttcaaattt gtactagaat gaaatcaagg aggagataca aaatgagtgc aactgagaaa
     1981 attccttcta ttgaccgcaa gatatttaag atactgatta tgtgtagtaa aaaagagttt
     2041 gtgagtatta attcaatagc aaatgaattg aatgtcacaa ctagaagtgt aaggacgtat
     2101 ataaaacaat tgaataaaga tttaggtaat gatatagcag taatcaaata tataaaaggt
     2161 caaggctata aattggaaat aaaagatgag cagatactca ataagattat tgatatgaat
     2221 agaaaaaatg tattttcatt aaattcaaaa gaagatagag tagagtttat actaaattac
     2281 ttgatagaac tagatggatt tattacactt gatagtttag ctgatgagat gtgtgtagga
     2341 agaactactt tagtaaatga tttccagtat gtagaaaaag ttttggcttc atataattta
     2401 aacttaatta aaaaacagaa tacaggtatg aaattaaatg gaaatgaact tgatattaga
     2461 ctgtttatat taaatcagtt gtataaaaac tcaaggaaag atttcagtaa ttcaaagtat
     2521 tttaaaggaa tcaaaaaaga agaaatcata aagttagaag aaaaaatatt gactttattt
     2581 aaaaaaaata atttttatgt tacagatgaa atgttaagag aagttattaa ctacatagta
     2641 gttttagtct atagagtaaa agaagaaaag aaagtaaaag actatgatgt aaagtttgat
     2701 ttacttaaat catatgatga atactttatt gctagggaaa taaaacatat aatatcagga
     2761 atgtttgaat gcactttaaa tgatgaagaa attatttatt taactatacc attagttagt
     2821 ggaaatgcac cagcatcaga atgtgcttta aatagtagta gaattagtaa aaatattgat
     2881 gagttgatgg aaaatatatt taatcaaata tatgtcgata tgggtatttt tataaatgag
     2941 gatgaattaa gagtagggct aggatatcat cttagtttta ctctaaacag gctattattt
     3001 aatataaagt taaaaaatgt tttgttagaa gagattaagc aaaattatat acttccattt
     3061 aaattagcac aaatagcagg aaaagtaata gagaaaaagt ataacttaga agtttcagaa
     3121 gatgagattg gatatatagc aatccatttc agtggatatt tagaaagaaa tagcagtaga
     3181 ttctattcaa taaaaaaaat agcaattata tgtagtacag gtctaggaac agcaaaacta
     3241 ttgaaaataa gagtagaaaa actgatagga aataatccta aaattgatac tctttcaagt
     3301 tttaatttga gaaatattaa cttagacgaa tacgatatag tttttacaac tattgattta
     3361 gacacatcaa atataaatac aatagtatta aaaataaata caatatttga tgaaaacaaa
     3421 ctaagagaac aattaaaaac agtattgtgc ctaagagaag ggaatattga cactactaat
     3481 tctacaaatt tactaataaa taatttgtta gatgaagata aatttatgat attaaatgaa
     3541 aagactatac ttgcttcatt agaaaaaatg atggaccatc ttatggattt aggatgcatt
     3601 gatgataagt tcagaaaaaa tatagtcaat agagaagaaa aatctcccac agtatttgat
     3661 aaaggtttat tatttcctca ttctgtaaat gaaaaatcag acaagttttt aatggcagtt
     3721 ggaattttag aagaaccaat agaatatgca aatagaagta taaaaataat tcttatgaca
     3781 atgtttccat gtgagaataa gatagactca gacttattag taaaaattta tgaagaagtt
     3841 ttaaagatag gccaagatgt aaaattaaca aataagataa gtaaatgtag aactttttta
     3901 gaatttaaaa aggtgttact taaaaacctt atataagtat tgtacagcat ttatagttgt
     3961 tatacagatt taaaaataat gtgatttttg gaaggtgtat aaatatgaaa ttattaatga
     4021 ttttatttgt tgcttatatt ttaaatacta tatttataat gaagcagaat aaattgtatt
     4081 ttaaaacttt aaacagagct aaaaaaaagg gtgatatagt gtctacaggc aaaaagaaaa
     4141 gctatttttc taaaggaagt atagccatta tatcttcaga tttagatgga tttataaagt
     4201 ttggagaaat tttaaaaggg cgtacagtaa tggctaagtt taaagagatt gaagaaatag
     4261 aaggattaga tatatatagt gcagagcaaa aatttaaaga tgaagagagt attgtacaag
     4321 ctataaaatt tataaaagaa aaaataaacg tttcttttaa ttaactcaat ataaatataa
     4381 attatcagga ggaatgtcat gaaatatgaa gttaagataa gtgggatagg cagtttagtt
     4441 aaagagttaa tggatgaaag caattgtctt attatatatg atgaaaccat taatgatgac
     4501 gacttaaagg atatatctgt aattcattca atagcaacat taaaaagtga tgttgagata
     4561 ggagatacac ttactatagg gaatagagat tattgtatag tttcagttgg tgatattgct
     4621 cagaagactc ttagagaaat tgggcattgt acaatcaaat ttgatggaaa atgtgaagta
     4681 aatttaccag gagaaataca tgtggaaata ggaaatccag atataactat tggagattta
     4741 ataactattt cttaatttga aaataaaatt attataacag gaggaattgg tatgttagat
     4801 ttaaacagaa aacttgcaaa attggaagag gaaggtaaaa aaataaaagt agctcttata
     4861 ggagctgggc atatgggaaa tggaatggta agccaaatgg caaatatgaa aggtattgaa
     4921 gcttctattg ttgtagatat aaatttggag ttggctcata aagcttttac tgatgcagga
     4981 atagatgaag aaattataga taatgtaaca aatgaaaagg atgcagaaat aaaattacaa
     5041 gaaggaaaag tattgacatg taatgatttt cttgtggctt gtaaaactaa gtcaatagat
     5101 gtagttatag atgcaactgg gggtattgca attggtgcag agatagcatt aaactctatt
     5161 ttaaataaaa aacatatagt tatgttaaat gctgaaactg attgtgttgt tggaccaata
     5221 cttaaaaaat tagcagatga tgctggagta atatttacag gttctgcagg agatgagcca
     5281 ggtgcagtaa tggaattatt tgattttgca gatgcaatgg ggtttgaagt tagagtgata
     5341 ggtaaaggta aaaataataa attagattta gattgtaatc ctgatacagt tagagaagaa
     5401 gcagaaagaa aaggagcttc acctcatatg atagcttctt ttaaagaagg aactaaaaca
     5461 atggtagaaa tggctttaat gtgtaatgca actggatttg tgcctgatgt aagaggtggt
     5521 catggtattg aagctacagt taatgaagta cctaagaaat atgcattaaa aagcgaaggt
     5581 ggagtattag ataattatgg tgtagttgat tttgtaaatg gtatagcacc tggagttttc
     5641 gtagtagtag cccataaatt aaaagcagtt aatgatgaat taaaatattt aagtatgggt
     5701 gatggaccta attacatatt atatagacca tatcatttat gtagcttaga gactccttta
     5761 tcagcagcta tggcagtttt ggaacataaa gctacaatag tgcctaaagc tggattagtt
     5821 gcagaagtta tgactatagc taagaaagat ttgaaaaaag gtgactttat ggatggctat
     5881 ggagcatata catgctatgg tactatagaa aaatacgatg tagcaaaggc tatgaatgct
     5941 gtacctattg gtttgataag taaaaagact aaagttgtaa aagatattaa aaaaggtgaa
     6001 gttataactt acgatatggt agagatagaa aaagatacta ctttatatca tttaagacaa
     6061 ttgcaagaaa aaatatttgg gtaagatttg atggtaatat aaatagatat tagtaaaaag
     6121 ggttgatggt tatttttaaa tcaacccttt taaattttta tatattcttg ttaggagact
     6181 ttctttaaaa gtatattata ggttatttgt agtcataaaa attattttga attagttctg
     6241 caattttata ttcatattga aacaatatta ttaaaaattt taacataaaa tgaattaaaa
     6301 tgaccaatta tgtattataa tattaactgt aatactaata tagttctatt aattaaagaa
     6361 atatccccca ttaatatata ataaatatct ttaagtgaga gaacttagta aaaaatatat
     6421 cgtattcaat aaaaaatctg tttacagaat ggtatatata gttatataat atactatata
     6481 agatgcgaaa agataaaaag tatagcacaa tgaaagaagg tgattattat tcagcttaat
     6541 gaaagacaac taaaaattat agatatagtt aaggaaaatg aaccaataac gagtgaaagt
     6601 attgcctcca gcttgaatgt gactcgtgct acacttagat ctgatttagc tatattaaca
     6661 atgacaggaa tattagacgc aagaccaaaa gttggatatt tttattcagg agttagtgaa
     6721 ataaatttaa ttggtaaaag tgtaaaagaa aaaacagtag aggatataat gagcatgcct
     6781 gtactagcta agaaggatga aagtatatat gatgtaatag tcactatgtt tttatctgat
     6841 gtagggagta tagttattat agatgagaat gaagagttat gtggagttgt ttccagaaaa
     6901 gacttgctaa aagcaactat aggaggttct gatataaata aaatgccaat aggtatgata
     6961 atgacaagaa ctccaaatgt agtcacttta actaaaggag caagtgtttt attagcttca
     7021 agaaaaataa tagaacatga agtagattcg attccgattg ttgaatataa gggagaagac
     7081 aaaaatcata tgagagttgt tgggagaata tcaaaaacca acataactaa gctattctta
     7141 gaaatagttg acaattaagg aggtatgctg gtgaaaaacc taattatata tgcagtatca
     7201 gattcagtag gggagactgc acaacaagtt gcaaaagcat gtatgtctca gttttatgtg
     7261 aatgaaactt atgaaataaa aagatttcca tacatgatta ataaaggagt actacttgaa
     7321 actttggaga atgcaaaagc tgaaaacgct ttaattgtat atacattagt tgatgaagaa
     7381 ttgtgcagta ttgttgaaag atattgcgaa agagaaggtc ttagttgtat agatttaatg
     7441 acagatatac taagagaaat aagcaagaga acaggaagaa aacctaaaag agaagctggt
     7501 ataattagaa aattagatga atcttacttt aagagagttg aagctataga atttgcagtg
     7561 aaatatgatg atgggaaaga ccctagaggt attcttcaag cagacataat tttagtggga
     7621 atatcaagga catctaaaac tcctttgagt atgtatttag ctaataaaaa tataaaagta
     7681 gctaatgttc cattagttcc agaaatacca attcctaaag aagtgtttga gatagatact
     7741 aaaaagataa taggtcttac taattcgcct gaaaagctga atgagataag aacccaaaga
     7801 ctaaaagcct taggattgtc cagtaaagca aactacgcta atttagaaag aatacttcag
     7861 gagttagatt attcagaaga gataatgaaa agaattggat gcccagtaat aaacgtttca
     7921 aataaggcaa tagaagaaac agcaggtata attttagaca taatgaaaga aaatggtcta
     7981 aagatataca aagaaataga aatttaataa tgtctattaa taaaaatgat ataaattaaa
     8041 ataaaaagat taatggggga tttaacaatg gaaactaagt atgtttatag ttttggtgaa
     8101 ggaagcaaag acatgaaatc tttactagga ggtaaaggtg ccaatttagc agaaatgact
     8161 aagataggct tacctgtccc tcctggattc actataacaa cagaagcgtg taatgattat
     8221 tatgttaaca atgaaagtat aagagcagag ataataaaag aaatagaaga gcatttagct
     8281 actttagaaa aagacttaaa taaaacatta ggatgcaaca agaatccatt attagtatca
     8341 gttcgttctg gtgcagtatt ctctatgcca ggaatgatgg atacaattct taacctaggt
     8401 cttaatgaca atagtgttgt tggcttagca gaggctacac aaaatgaaag atttgcatat
     8461 gatagttaca gaagattcat acaaatgttt tctgatgttg ctatggaagt tccaaaatat
     8521 aaatttgaaa atgtattaga tagaataaaa gaagcaaaag gatacacagt tgatacagaa
     8581 cttacaactg atgatttaaa agaaatagta aaagaattta aagcaattta taaaaaagaa
     8641 ataaaaaatg atttcccaca agacccaaaa gagcaattaa tgcttgcaat agaggctgta
     8701 tttagatcat ggaataatcc tcgtgcaatc gtataccgta aattgaatga tatagctcat
     8761 aacttaggaa cagcagtaaa tattcaatca atggtatttg gtaatatggg agaaactagt
     8821 ggtacaggtg ttgcatttac tagaaatcca gctacaggag aaaataaatt atttggtgaa
     8881 tttttaatga atgcacaagg tgaagacgtt gttgcaggta taagaactcc tcaaaatata
     8941 tcaactcttg cagatgtaat gccagctgta ttcgatgaat ttgtaaaaat tactcatata
     9001 cttgaaggtc actataaaga tatgcaagat atagagttta ctatagaaaa tgaaagatta
     9061 tatatactac aaactagaaa tggtaagaga acagcagcag cagcaataaa tgtagcagtt
     9121 gatttagttg aagctggaat aattgatgaa aaagaagcta tcatgagaat tgagcctaat
     9181 caattagacc aattattaca tccaaaattt gaagataagg cattaaaaga agccaaggtt
     9241 atagcaaaag gtttaccagc atcaccaggt gcagcgagtg gtaaagttta tttcaatgct
     9301 gatgatgtag taaaagcaaa cgaaaaagga gaaaaagtag ttttagttag actagagaca
     9361 tctccagaag atatagaagg aatggttaaa gcagaaggaa tccttacagc tagaggtgga
     9421 atgacatcac atgcagcagt tgttgctaga ggtatgggta aatgttgtgt tgctggatgt
     9481 ggagaaataa aagttgatga gtttaataaa gaaataagag cacttgatga tgtagttata
     9541 aaagaaggtg aatacatttc tatagatggt tctacaggaa atgtatacct aggagatgtt
     9601 aagaaaacag aagtatcttt aactggaaac tttgaaaaat taatgaactg ggtagacaaa
     9661 cataaatgta tgatggttag aactaatgca gataatccta gagatgctcg tgcagctata
     9721 gagtttggtg cagaaggtat aggtctatgt agaactgagc atatgttctt tgatgaagct
     9781 agattgccag cagttagaga aatgatatta tctaatacag tagaacaaag agaaaaagct
     9841 cttgaaaaaa tattaccaat gcaaagagag gattttgtag agttatttaa agttatggat
     9901 ggaaaaccag ttaatataag actactagac ccaccactac atgaattttt gcctcatgat
     9961 gatgagacta tagaagaact ttcaaaatct atgggaataa aagtaagtga cattaagaag
    10021 agaatagttg atttagacga gtttaaccca atgcttggac acagaggttg tagacttgca
    10081 ataacttatc ctgaaatatg tgtaatgcaa tctaaagcta taatacaagg tgctatagaa
    10141 gcaatcaaag caggtgttaa agtttctcca gagataatgg ttccattagt tggagaagta
    10201 aatgagttaa aaataataag aaaaatgata gtagaaactg ttgacgctat aataaaagaa
    10261 gaaggtgtag aagttcctta tactgtaggt actatgatag aaatacctag agcttgttta
    10321 actgctgatg agatagcaca agaagcagac ttcttcagtt ttggtactaa tgacttaact
    10381 caaatggcat ttggatattc aagagatgat gctggtaaat tcttaggtca atatgtagat
    10441 gaagaaatat tagaaaaaga cccattccaa gtactagacc aaaatggtgt tggtaagtta
    10501 gttaagatgg gagctaaatt aggtagagaa gttaaaccag aattaaaact aggaatatgt
    10561 ggagagcatg gaggagaacc atcttcagta gagttctgtt atagtgtagg acttaactat
    10621 gtatcttgtt caccatttag agtgccaatt gctagacttg cagcagctca agcatctata
    10681 aaaaatccta gataataata gactgcaaaa tatgtaacta aataaaaata aaatccccta
    10741 ttgttcaatg tatcaaatta agtatttgta aattgaatat aggggatttt tattataaaa
    10801 atttaactta tatagttgta agtgaaacgt atttaattat attgcatata ttattgtaga
    10861 gagaaatttc aactatcaat taaattgagt ttactggagg ggataaagtt gacaaagtgt
    10921 cctaaatgtg gaaaggaagt atcatcacgt ccaggtagta tatgcccacg atgtggattt
    10981 aaagtagcag atgagagtct aaaaataaga tgtccagaac ctagttgtag ggctttggta
    11041 tctaggaaac tggattattg tcctaaatgt ggatgcaaac ttagaggata taatatttct
    11101 gaatttgtta acatggtgag atgtaaaata gatgagacat ttgataaaag atagttaaac
    11161 ttagagttaa gattttgagg gtgtctcaaa atgaactttt tagttcataa ggcactcttt
    11221 ttgtatggaa tcaaatatat ttttataatt taaacaataa aaaagaggct tatctctatt
    11281 ttgagagagc ctctataaaa gtatacagtt ttataagtaa atatattttt gttaatatat
    11341 ttctattatt tgccttctga atcttccatg attaaagtca cgagtgtgca tagctatttt
    11401 ttaatcaaat gtatgttaaa gtaatctttc tatttaccag aattactttc cagtattaag
    11461 tatccaaggt ttttctatac cattttttag tgagttataa ttcacaataa gagtgtcata
    11521 gctattttta gcatcttcat tacttacaac ttgtttttta taatcagtta gagtaattaa
    11581 tcctaaatca tatcttaatt tagtattttt taattgtttt tctgataaat caagttgttt
    11641 tttagtaatc tgaattgtat cttctaaagc aagtaattga gcatagctat caattaaccc
    11701 ttttttaaga gcttttttac tgtcttctaa tgttactaga ccattttcag tattaaattt
    11761 ttgttgaaga taatttgcat aagtagtaag accagcagca taagtctgtc tttcaagatt
    11821 gtatgcttca acttcttttt catatttctc gtagtctttt ttatacttat ccataagaac
    11881 tttgtattca tcatcagtta gactaccttg ctctggtttt gtaggagcaa ctggtcttgt
    11941 aaggttaggt ctatctggta aatctgcttt gttctcatct ttatcattaa aactttcttc
    12001 tgtacgttcc aaaatcaatt ggtcgtattt taaatattca ttaaccttac cttccatata
    12061 tgaatcaact gagccaccta ctctaaaaga ttcaaatcta tattctttat ctaattgata
    12121 attatttaaa tctatattag tcaataattt aaaatagtct tgattgtttt ttagttcttg
    12181 aagtttagct ttttgagtat tttgtagtgt ttgaagttca agttcagctg attcagtttc
    12241 taaagatgtt accagcccta aatctttttt taatttcata tctttaattt ctttagtctt
    12301 tatctcaaga ttgtttttta tcttatctag ctcattttct cttgaaacta aatcgttaaa
    12361 tttattagta atatcatttg ctatttggtc ctccataaag cctttagcct gttcactctg
    12421 atttttaaga agtccaattt ggtcgtatgg aaacgagttg tcacttccag taaaactatc
    12481 aaactcatct tgtacttcta acttttcttt taacattttt atttcttctg atttcatttt
    12541 taatttttca ctatttttaa gtgcagcact aacagcttct ctagcagtca aaatttcctt
    12601 ttcagatgtt cttttatttg agtcacttaa aatagaatta tcaactttgt ttatagaaga
    12661 agtattatta tctgcgtaaa ctgggattaa attggcagtt attataccaa caccacagcc
    12721 aattgcaact attttgctca ttcttttatt caatacaatc accctccatt ctatttaaac
    12781 tttagctttc aattttggtt tctttattat tttacgtttt atacttccta ttttatctgt
    12841 tatcttacca aatattttag gcattttgtc tgaaaactta ttttcccatt tatcaaatat
    12901 taagtatata gtaggtataa gcaccaaagt tataagtgta gatacagtta gaccacccat
    12961 tataactatg gctagtggtt gcatagtttc tccaccttca ccaagagata gtgctgttgg
    13021 caacatacct aaaattgttg ttgctgtagt cattaagact ggtcttaatc ttgctgcaca
    13081 accaattgaa accaaaccaa ttaaatcatt gttacgattt gtttttctaa gttgctctat
    13141 gtagtcaata agaactatac cattattaac aacaataccg ataagtaaga ttatacctag
    13201 cataccgaca gtacttaaac tttgaccact aactagaagc gaaagcacaa ctccaacgaa
    13261 tgcaaatgga acacagaaca tgattataaa tggtttacta aatgattcaa actgagctac
    13321 cattaccata taaactaatg ctatagctat gaccattgaa aatccaagac ttgccataga
    13381 ttcattcatc atttctgttt caccaccaac acctatagag taatctttag gaattaccat
    13441 tttattagtt ttttctagcg ctagttttgt agcagtacca gcatctaaat tgtcaagtgt
    13501 agcagttata tttacagaaa aatctccatc agtcctagat atagattgta gaccatctgc
    13561 catttttata tctgaaaaag caccaagagg aacttctttt ccagttgcag agtttacttt
    13621 tagttgcttt atatcttcta agctatcaat agtatctgct ttaaatttta ggtttacatc
    13681 cactttgtaa tcatcaatag tagcagtagt gacggagtca ccatttattg ctgtacgtaa
    13741 catgcctgca atttcagcag tatttacacc atattcttga gccttatgtt tatctatagt
    13801 aaattgagct tcttgtgttg tatctgtaag agaagtttca acatttctaa atccttttat
    13861 agagctaaat tctttttcag cttcttttcc cagtattttt aatacatcta aatcagggcc
    13921 tttcatttct aatgtgaagt ctgctcctcc agctccaccc ataacactac tgttttgact
    13981 tacatttatt ttacaatctg gtacagtagc cactctatca attatctctt tttctatttc
    14041 atcagtagtt ctttttctat ttttttgaga ttctaattca attgctatac ttgctttact
    14101 attatttgaa tctgatgtca aagttgttat taaagtttta atttctggaa tatctgagac
    14161 ttttttctca gtcatagata catagtaatc attggattct aggctaagac cttttggaag
    14221 ttctatagaa atagataatt cacccttgtc tgctgcaggc ataaagtcca tacctataaa
    14281 tcccattcca aataaggaag ctacaaaaat aagtaaactt gatattacaa ctaagcgttg
    14341 atgtctcaaa catacactta ataatttttt atatgcttca cttaccttat caaatatagg
    14401 actatctttt tcttgcatct tttggctatt ttttcctctg cttaattttg caaatacact
    14461 aggaacgagt gtaattgcac ctataaagga gaatgctagg gaagctatta gtgttttacc
    14521 tagtgcccca aaagttattt tggctatacc ttctgtaaac atgataggta agaatattgc
    14581 tatagtggtc aatgttgatg caagtattgc atttgttaca gttcttgttc cctcaatagc
    14641 acaatcatca agactcaatc tgccactttt cctatattta aatatatttt caattacaac
    14701 tgtagcatta tcaacaacca taccaactga aattaccaat gaacttgcag taataagatt
    14761 taaactttca ccagtaaagt agagaattgc tatagcacct attatagaaa ttggtattgc
    14821 aactgcaaca actagtgaag ctcttacact ttttaggaaa gcaaatatta caataaatgc
    14881 gataacagca cttataagca agttgctcat tacgctgttt attgattcac ttatgtatga
    14941 acttgtatca ttagcaattt taattttaaa ttgaggattt tccttattca attcatctac
    15001 gtatttgtat gcactctgca taacttttac agtgttggca tcttgttgtt tagaaatatc
    15061 tattaccaaa ctgtcctttc cattgtatct ataaatacta tctttgtctg catatccata
    15121 atctacacta caaagattat ctaatctaac tgtatttcct ccttttacag gaatttgaat
    15181 ctgttttata tcatctaggg attctaattt atcaattgct cttaaaacta ttttatcttc
    15241 accttgagta attgaaccat aagggaatgt tttattacta gaggataaga caccttttat
    15301 agtggataaa ctaactccat aatttgaaag aacagctggg tcagctatta cattaacctg
    15361 tgctttttct ccacctttaa ggtctgctga agtaacacca tctatggatt ctattttagg
    15421 ctgaattaca tcttcagcgt attttcttaa ggcttgtgaa ctattttcat ttcctccaga
    15481 tatgacaagt tgacctatgg cctgtgcatt catatccagc tttaaaactg ttggtttagc
    15541 tgcatcatct ggaaggctgg tctgtaatgc atcaacttta gacctaacat cattcattat
    15601 ttcatccaaa ttagttccaa attcaaactg agcaactacc atagaaacac tttcttgtga
    15661 acttgatata gtagatttta catttgaaac ggcagataaa ctttcatcaa ctttttgact
    15721 aacttgttca tcaacgtctt gaggaccagc acctgtccaa gtagtcatga ccattacaac
    15781 tggtatttct atatcaggca ttagatttat tgacattttt ttatatccaa taccaccaaa
    15841 aactattaat actaaaaaga ccattatgat tgtgagagga cgtttaacgg aagtctgtgt
    15901 taaattcata gtctacttct ccttttctac aggaaatatc ttagttccgt cagaaataag
    15961 acttatccct ccaataatca ctgtatcttt atcacttaca ccacttttaa tttctatttt
    16021 atcctctgtt acaattccag tttgaaccaa gtgtttcttt gctatattgt cttttgttgc
    16081 tatgtataca tattttttac cattttcttc aaatattgct tttttaggca ctgtaacagc
    16141 accattttct ttttcaatac ttaagtttac ttgtgcagac atacctgcct taaatttgtt
    16201 gtttgtatta tttacaagta ctttaacagg atataaagat gtctgagggt caactactga
    16261 aggtacataa tctatttttc ctaaaatcct ttggtcttca attataacat ctacatttcc
    16321 atctttctta aatttaccta tatctgattc tgctacattt aaatcaactt ctagtatatt
    16381 aggattagaa ataataaaag ctggttgtga ttgtgttatc atttcctttg aatcaaagtt
    16441 tttagctgtt attgtaccat ctactggaga tgttaatgtt aatttgtcta agttactcat
    16501 tgctaaatta tatgatactt gagcttggtc aagctgtttt ttagcaagtg ccttagcttg
    16561 aggtatactt tttgaagttg ctgtatcgta tgctctttga gcagaatcta actgttgttt
    16621 tgtttgctct aaagaaaact ctattttttt aaattggtct aaactaatga cttcctgttc
    16681 ataaagaatc ttattattgt cataatttct ttgagactcc tcatattgaa tctttgcatt
    16741 gtcaagagcc gtttttgcct gatttacttg tgcctctaat gagccaccag tggcgcttga
    16801 ataacttgct tttgctaaat ctagtgaggc tttactttgc tttacactat tttctagctc
    16861 tggactgctt atactcaata attcatctcc ttttcttaca ttttgcccaa gttttacata
    16921 cacttcttga actgtgccaa cagtctgtgc agtaacagct gtttcatctt taacctttgt
    16981 agttcctgag aaagaatttg tgttttcaat ttctccacct actgcttttt gaacagaaac
    17041 tgctattggt ttctcaggtt cagattcttc tgtttttcca catccaacta aaaacagtga
    17101 actacacatt aagaaaacta ttaaatatct tttcatatct cctcctatta tatattgcac
    17161 actagcttgt tgtagaattt aaagtgtctt ataatttaag tttagtttta gactaattaa
    17221 aaaacttccc tttattggca atttataatt ctctaatttg attataaatt tataattatt
    17281 atgagtcaat aactttttct tgttttaaat ttattaatag tttataattt atactatatt
    17341 aaacaacaat gttttattat attatctatt gaagggatgt tacaaatatg gataacaaca
    17401 atatacttaa aatagcggac tcttttttag attttttatt tgtgcttgaa aataatatat
    17461 ttaaagaaag cgatttgtta aaaaagtttc aaaataactc tgatataatg aaagcttatt
    17521 ttgaagaatg tccaatggct ccatcgcatg ctaaggtaat tctttatctt atgacatcaa
    17581 attcatcatc aatttctcaa atagcttcta atttaggaat tttaaaatca aatatgactc
    17641 cgattattga cagattagtg gaacatggtt tagtcaataa atttcctgac ccaaaagata
    17701 gaaggattct tagggttgaa ttaacggata aagcatttga attatttgat gcagtaaaag
    17761 ctattctcaa agaatctcta gtaaaaaaat tatctaatct ttctgaagag gaactaactt
    17821 tattagatga acacacatta aagttatcag aaatagtaaa aaaattgggt tagaatgtga
    17881 ttgtaaaagt cactattaaa aaagttaaaa cttcataaga aaagaaatag cagtagtata
    17941 atatagatta ttatactact gctatttctt ttcttatgaa tatttagttg tcaaactcta
    18001 ttttccaatt gaaatagcca gtatctgggt ttttattata ctttaataaa gcatcaaatt
    18061 tattaccatt tttagcggtt aaagatttta ctttagtttc accttttttt aaaatatttt
    18121 tgaccattgt tttgttaact ttctttttat aaagacttaa atacttatca tttttccaaa
    18181 taacaaattt acattcttta tgatttttac aagcaaagcc tttgtcaaat tccagtacat
    18241 caccttgaca aacggggcat ttgccaagag atggattttc atcatcattt ttaacttctt
    18301 ttttgttatt agaatctttt gatatagcaa cttctttttc agcattagtt acatcttttt
    18361 tctgcttttt ttcttttgtt ttagtaaatt ttttagtttt tgaatcgaaa gtatatgctt
    18421 ctgtgtttat attttttgaa gcatcttgtt ttataatatt gacattttga tatataaaac
    18481 taatgatatt agtaagatat tcttttcttg tatactttcc tttttgaata tcgcttaaac
    18541 ttttttcaag tttcccagta taatctacat caaataagtc tttaacagga aaaatttcaa
    18601 ctaaattttt cccaaggtct gttatagaat aagatttccc ttttttcttt acatagccta
    18661 cttgacttat cttctttaac acatcagcac gtgtggctga tgttccaata gaatatccag
    18721 acagcactgt tgtatcttct tcaggaacat ttttaccaca atttttcata gcctttaaaa
    18781 gggtgtcctc agtatacggt ttaggtggag tagtttgctt agttaatggt tttatttcta
    18841 acacatctac aacatcatct ttatttacaa gtggaagtaa atcgtttttt tcttccttat
    18901 tatatacctc aagatatcct tttgatttaa gcaccttacc ttttgttaag aaagtacaat
    18961 tatctacttc agtcttaatt tctgtattct catattcagc aggaggcata aagtttgcta
    19021 caaatctgtc ttttatggca tcataaacta attgttcatc cttagataaa ctttttggaa
    19081 ttatataagt tggaattata gcactatggg agtctacttt agaagaatca aaaactcttt
    19141 tagttttaga aaatttaatt ttattttcat attcaagacc aacctttaat ttatctaaag
    19201 tttgagaagc ttttgaagcc aaactttctt ctaagaaaat ggaatcagtt ctagggtaag
    19261 ttatgtatcc acccttacca ttaccctcat agagagattg acagacattt aaaaccttat
    19321 cagatgtaaa attggaatac ttagaagtta tatatccttg aagtgatgtc aaactaaaaa
    19381 gttttggtgc atattccttt gacatagtaa cttttttatc tagtatttta ccagtttcag
    19441 atgtaattga agctataatt ttatttgcat cttcaagtgt atcaaattta ctttctttac
    19501 ccttaacata tttaccttta tattcaccag cttcagcttt aaaatgccct tctatttcat
    19561 aataagtttt aggaacaaag tttagtattt ccatatctct atcatagact aatttaactg
    19621 ttggaagaat taccctacct atgtttaata gtttcccatt tccgtatttc aacgtagcaa
    19681 cagaagtgaa atttattcca atcaaccagt cagtaataag cctagtatat ccagcagctt
    19741 gaagatttct catttcatct tcatctttta ggttttttat acctcttgta atatcttctg
    19801 gcgtccattc atttaccaag attcttttta ctggtttttt attttttgcc agtaaaaata
    19861 ttaaaaatgc tataagttct ccttctcggt cattgtcagt ggcatttatt acatattcta
    19921 cgtcttttct attcaaaagc ttttttacag tgttgaattg tttagttttg gaactatcaa
    19981 tcttaaattt aaacttatcc tcaggaacaa atgggaaatt gttcattttc caagagccag
    20041 agtatttatc tttatcataa tccttcatat catataatga tactaagtgt ccaactgcat
    20101 aagtaattat ataatcattt ccttcaaagt aaccatcttg tctggtcttt gcacctaaaa
    20161 atgatgctat agtttttgca actgatggtt tctcagcaag tacaagtttc ataaaaaatc
    20221 ctcctttaaa acactaataa ttatattacc acaaaaaaat aaaaaacttc aagaggtcga
    20281 aatttaagtt ataatattta tagaaagttg aaaggagata taagatgtac aatttaaaag
    20341 taaaaaaatt aaatgatgat gctataatac ctaattttgc tcacaaaggt gatgctggta
    20401 tggatttgta ttctatagaa gaggttgtaa taccttctgg agagactaaa cttatcaaaa
    20461 ctgggatatg tatagagctt cctactatga cagaagcaca ggtaagacct agaagtgggc
    20521 ttgccttaaa gcattctgtt actgtattaa atacaccagg aactattgac gaaggatata
    20581 gaggagagct taaaattata ttaataaatc atgggaaaaa tgattttaag gttgaaaagc
    20641 atatgaaaat tgctcaaatg atagtaaaac ctatctatga tattagcata gaggaagtaa
    20701 aagaattaag tgactccgaa agaggaaaag gtgggtttgg ctcaactgga ttttaaaaag
    20761 ttacaaaaaa gttaaaaaat gtagccattt ttaaaaaagg taaaagatgg ctatattact
    20821 aatttacagg ctattaaaga ttaaaattta tacatggtaa tttatggtaa gcaaatatta
    20881 ctatagacaa atagttacaa atttgtaata atattaatat aaaagaaata attgatattt
    20941 taatagtgtc aattatttct tttatatttt gaagtgaaat aaagatagta gaaaagagga
    21001 gatgagtgga aagttatgaa ttttaatcaa aagagaatag cagccagtat aatggcaact
    21061 gcgataataa tgcctactat ggggaattta gcatatgcta atgagtctga agtagagagt
    21121 gtgagtatag aaagcagaac aataacaggg aatgcggtaa attttagaaa aggaccaggt
    21181 acaaaccatg aatcaatggg gaaactttac aaaggtgata aagttgagta tgtaggaaaa
    21241 gaaggttctt gggtaaaagt taaatataat ggaaacacag gttatgtgca tggaaattat
    21301 gtagcaataa atagccttgg aagtagcaat gaaagtagtg atacttcagt taagtcgaca
    21361 aaagttgtaa cagcaaaagg attaaatttt agaacaggac caagtacaag tagttctaaa
    21421 atatcaacac taggatatgg gactgaagtc ggatacatat ctgagtcaaa tggatggtca
    21481 aaaataagtt ctaatggtag agtgggttat gtttcaagta aatacttagg aactagtgta
    21541 aatgactcta caaatgaaaa tgctgaaaat agttcaaatg accttgtaaa gggtacaaag
    21601 gttgtaacag caaagtcatt gaacgttaga acagggccag gtacaagcca ttctaaaata
    21661 gctacattaa gttatggtac tgaggttgga agtatttctg aaagtggagg atggacaaaa
    21721 gtaagttatg gcaatcaaac tggatatgtg tcaagtcaat atcttgctga aaaaggttct
    21781 gttgatactt caattccatc ttattcgact aattctccaa gtcaaggagc tgaaagtgta
    21841 ataagttttg caaagacact acttggaaaa ccatatgtat ggggagcaga aggtccaaat
    21901 agttttgact gttctggatt cacacagtac gttatgaaaa aatctgcagg agttagtata
    21961 ccaagagtat ctagagacca aagtaagtat ggaacatatg taaatagagg agaccttaga
    22021 agtggggact tagtattctt tgatacacaa ggttcaaata atggttctgt aagtcatgtt
    22081 ggaatataca taggaaatgg agatatgata catgcatctt caggttcagc aaaaaaagta
    22141 acaatatcta acataaatag tagctactat tcaagtagat atgtaaacgc aagaagagtt
    22201 ttataattat ttttattata aaacataata aatagatttt tctttattgc ttataatatg
    22261 aaagcaggta caatattgat agtaatgtat agtataatac atgttactat ttgattggta
    22321 cctgcttttt att
//
LOCUS       DAEMAE010000045        21916 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEMAE010000045 DAEMAE010000000
VERSION     DAEMAE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.046"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..1144
                     /locus_tag="KPH93_001387"
     CDS             32..1144
                     /locus_tag="KPH93_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG1688216.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLVFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KPH93_001388"
     CDS             1398..3422
                     /locus_tag="KPH93_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1688217.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQN
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPIGPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRTNNYPAVLVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KPH93_001389"
     CDS             3642..4328
                     /locus_tag="KPH93_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG1688218.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KPH93_001390"
     CDS             4423..5478
                     /locus_tag="KPH93_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG1688219.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEDFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="KPH93_001391"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="KPH93_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG1688220.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTIFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="KPH93_001392"
     CDS             7194..8900
                     /locus_tag="KPH93_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG1688221.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKSVRRVLSECGFKNVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KPH93_001393"
     CDS             8922..9995
                     /locus_tag="KPH93_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG1688222.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KPH93_001394"
     CDS             10007..10729
                     /locus_tag="KPH93_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1688223.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KPH93_001395"
     CDS             10745..11902
                     /locus_tag="KPH93_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG1688224.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KPH93_001396"
     CDS             11905..12744
                     /locus_tag="KPH93_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1688225.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KPH93_001397"
     CDS             12759..13934
                     /locus_tag="KPH93_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG1688226.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRNIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KPH93_001398"
     CDS             13963..14712
                     /locus_tag="KPH93_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1688227.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KPH93_001399"
     CDS             14734..15642
                     /locus_tag="KPH93_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1688228.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLKIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KPH93_001400"
     CDS             15690..16442
                     /locus_tag="KPH93_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1688229.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="KPH93_001401"
     CDS             16420..17763
                     /locus_tag="KPH93_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG1688230.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWSREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKVKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="KPH93_001402"
     CDS             17769..18860
                     /locus_tag="KPH93_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1688231.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLTKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWQNETGLIVEPKQPEEIKNAIIKLMENDELRLSMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KPH93_001403"
     CDS             18944..20857
                     /locus_tag="KPH93_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG1688232.1"
                     /translation="MLESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFITNDIIISACVYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KPH93_001404"
     CDS             21056..21763
                     /locus_tag="KPH93_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1688233.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
BASE COUNT         8726 a         2286 c         4062 g         6842 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaagataaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact ttcttatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaag acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctactaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tgggagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttgtatttt ctttattgta
     1081 tgtactattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagac gctaaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagagtta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgctgtctg
     1441 tggcaatggt ggcgccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagtataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacagggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc gccaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tttaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaaata
     2401 catataattg gctatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggg acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccacaataca aatttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaatagg accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaccaat aattatccag
     3301 cagtacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagct gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt ggcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagggtaac aagaaattat ctaaaagagc taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag attttgaaga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagtaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt tcttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacaatatt tgcagcagga
     5941 tttagaaacg accctgctaa gtttcaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgctg gtaaaaatgt atggatactg ccagcagggg cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtccat aaaagaatta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gatagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggttctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atccaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt cggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ccgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatccaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcaaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taaaaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacgcct attcatggta
     7921 caggtaataa gtcagtaaga agagtcttaa gtgaatgtgg ttttaaaaat gtgatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtgga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtagctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcgg atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta aattgaacaa tttgatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcgacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttatga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atacgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg cttctaaaga cattgccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatat ttggctgaaa aggggaactt
    11581 tctaaaacct tttggaaatt catatagccc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagt tctaaaaaag gattgagtta taaccgaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaggatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtataagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagaa
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaga gaaaacctat gaacaaaaag ttcaaaaaca
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgataaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaattagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttaaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattgagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaggaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattc attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatggtc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggggct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcaggaa taggatatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaaa ccagatactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aagttaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagagcta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt cgataaaaca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata cggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttaactaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag taagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatggcaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta gtatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgctag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta tcactaatga tataataatt tcagcttgtg tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgttacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttatggc aactttagct tatttaattc caatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gtgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aagaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aatcatccat aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagatttgtt gggcagagag cctataaaaa caaatttaag
    19801 agagataagt aattatattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aacaatgctt actcaataca acaagaatta cttagaaagt ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tagtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ccaagaaatt tgtacttata tctacagaca aggctgtaaa
    20221 ccctactaat ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtacttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggt ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggagctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaatt taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactaggga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgtcga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttga acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaagccag gagatttagt
    21601 tttctttggt tcaggcggtt caataaatca cgttggattg tatgtagggg attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttt
//
LOCUS       DAEMAE010000046        21670 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEMAE010000046 DAEMAE010000000
VERSION     DAEMAE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.047"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            95..964
                     /locus_tag="KPH93_001405"
     CDS             95..964
                     /locus_tag="KPH93_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1688234.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPPLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            complement(1076..1882)
                     /locus_tag="KPH93_001406"
     CDS             complement(1076..1882)
                     /locus_tag="KPH93_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG1688235.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGYTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKAGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKRYREDIPYLKDRKSSYNI"
     gene            complement(1896..3128)
                     /locus_tag="KPH93_001407"
     CDS             complement(1896..3128)
                     /locus_tag="KPH93_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG1688236.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMVLMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            complement(3588..5375)
                     /gene="aspS"
                     /locus_tag="KPH93_001408"
     CDS             complement(3588..5375)
                     /gene="aspS"
                     /locus_tag="KPH93_001408"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG1688237.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVYERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELTLPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKASLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            complement(5449..6711)
                     /locus_tag="KPH93_001409"
     CDS             complement(5449..6711)
                     /locus_tag="KPH93_001409"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG1688238.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            complement(6701..8257)
                     /locus_tag="KPH93_001410"
     CDS             complement(6701..8257)
                     /locus_tag="KPH93_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG1688239.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFNDSKANNNMESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDNESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDETLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            complement(8348..8962)
                     /locus_tag="KPH93_001411"
     CDS             complement(8348..8962)
                     /locus_tag="KPH93_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1688240.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHIAEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGNFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            complement(8977..9426)
                     /locus_tag="KPH93_001412"
     CDS             complement(8977..9426)
                     /locus_tag="KPH93_001412"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG1688241.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            complement(9451..11658)
                     /locus_tag="KPH93_001413"
     CDS             complement(9451..11658)
                     /locus_tag="KPH93_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG1688242.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            complement(11732..12244)
                     /locus_tag="KPH93_001414"
     CDS             complement(11732..12244)
                     /locus_tag="KPH93_001414"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG1688243.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            complement(12257..14707)
                     /gene="recJ"
                     /locus_tag="KPH93_001415"
     CDS             complement(12257..14707)
                     /gene="recJ"
                     /locus_tag="KPH93_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG1688244.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRGTFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            complement(14720..16384)
                     /locus_tag="KPH93_001416"
     CDS             complement(14720..16384)
                     /locus_tag="KPH93_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG1688245.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERIKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLRTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(16682..17179)
                     /locus_tag="KPH93_001417"
     CDS             complement(16682..17179)
                     /locus_tag="KPH93_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG1688246.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKRYIDESYEWYLEAA
                     QNQKY"
     gene            complement(17208..18587)
                     /gene="scfB"
                     /locus_tag="KPH93_001418"
     CDS             complement(17208..18587)
                     /gene="scfB"
                     /locus_tag="KPH93_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG1688247.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(18892..19038)
                     /locus_tag="KPH93_001419"
     CDS             complement(18892..19038)
                     /locus_tag="KPH93_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG1688248.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(19068..19646)
                     /locus_tag="KPH93_001420"
     CDS             complement(19068..19646)
                     /locus_tag="KPH93_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG1688249.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENLNI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKIAIFISTVVLLITILSL
                     SLSIFVDYFKYSAFAMFWIFVMLVLGKLKAKI"
     gene            complement(20014..21444)
                     /locus_tag="KPH93_001421"
     CDS             complement(20014..21444)
                     /locus_tag="KPH93_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG1688250.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEDDSKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     QAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTEDICIEAVRGSFEELEIKE
                     ILSYVKVPTERIFIEAVRQNGKILKYVESQTELMCLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKVVKQDYRMLKYINNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         6789 a         3981 c         2230 g         8670 t
ORIGIN      
        1 ttattatata aactttatat tataatgtat aatgtggagt tgattataaa attgatataa
       61 ctcaagtcat attaaaagag gtgtatttta tactatggat ttattgcatc ttaagtattt
      121 tcaaactgtt gctagaatgg agcacataac aaaagcatca cacaagttaa atatagcaca
      181 gccagcttta agcaaaacta tatcttcctt agaaaaagaa cttggggttc agttatttga
      241 cagaaaagga agatacattg ttttaaatga gtatggaaga ttgtttctaa aaagagtaga
      301 tagcatttta gatttagtag aaagttcaaa aaaggagtta caagacactt ctttagaaaa
      361 ttcaggagaa gtaaagatat tatctcctgc agcggctaat gtgttaccac cacttttgag
      421 taatttcaga aaactatatc ccaatattac ttttaatgtg tcacatactt tgccatcatc
      481 ttataaaaaa agtgattttg atttgtacat atcttcttca tttacaaagc taaactcaga
      541 aaatagcata actttaacat gcgaagaaat actattagga gtttcaataa accatccttt
      601 atcattaaag gatgaagtat atctaagcga agtttcagat gaaaacttta tagttataac
      661 taagggtgag aattatagag aggtaattga tattctatgc gaatcagcta atttcaaacc
      721 caaaatagca tttgaatctg attcaccata tacaatttat gctcttataa aatcacttca
      781 aggagttgga tttatatgtg gaaaatcatg gggattatct caagaccctg agattaagtt
      841 gttacatatc aaagatattg aatttaaaag atatttaaat ctttcatggt ttagcgaaaa
      901 ctatgaatct aaagctgtgt tgttgttcaa aaactttctt atcaactatt ttaaaaatat
      961 ttaaatcaaa gttgataata ttaaagagga tatatcaaag tataataaat ttaatcttaa
     1021 tattgataca tcctcttttt atcattttat attgaacttg cattataaca tagaattata
     1081 tgttatagct actttttctg tctttcaaat atgggatatc ttccctatac ctttttattt
     1141 catctaaatc aatctctgat aatacatact tttccctata cgtatctaat tgagttatta
     1201 cagtacctct aggattgcat attttactct ttccaaatag tattaaatta gagtcattac
     1261 ttactaggtt tacacctact gtatatacag tgttctctaa agctctttga gatacattta
     1321 aatcccacat atcttcatct tgtattctcc atgcagaggg tataaatatt atttcagaac
     1381 cttttaacgt caattctctg gatacttctg gaaagcctgc atcataacat atcattacac
     1441 caaatctgcc aaaatcagca tcatatacct ctactttttc ccctgcttta aagtatactg
     1501 cttctaatga ccacagatga tttttacagt attctcccat aatcttacct tttctatcga
     1561 atataactgc tgaattataa acatgtgtat accctttttc taaggttcca tatggagata
     1621 tcaggtatac attatttctt ttagcagctt ctgacattga ttcctctatg tacttattat
     1681 gttctcttat taattctaat gttttaactc cacctaaaga ctctaaatta tatcctgtag
     1741 caaatagctc tggtaaacaa attatatctg ccccttgttt tcctaagtca tctatcatct
     1801 ctactgcttt ttctatattt tttttaacat ctccaagtac actgtgttgt tgaattatac
     1861 ctatttttac cttacgaccc atgtaattgc cccctttatt tatctttgat tccaaaacat
     1921 ttcattaata tataataaat aaccatactc actacaattc catttatcgg aggtattaag
     1981 aaatttattt ttgaactata atacgacgct gcaaaaccta ttaaccagct tattactgca
     2041 atccaattta ttccttctct atgcttataa gttcttcctt gtataaaata atactcacac
     2101 cacataattc ctgctattgc aggaactgcg attcccaaga aagataaaaa tgactgaaat
     2161 ttaaatatta taccagtagc tgctattatt gtacctatta gacctgcaac taaagtcaaa
     2221 acatttcttt ttacatttgg aaaaatagtt tttaatgcta atccaccaga ataagcattt
     2281 gatacgtttg tcgaccaaga tgcaagtatt aaaataacaa acgctaccca tccaaaacct
     2341 aaattagcca atacttctgt tatgtcccaa gtaggtgctc ccatggacat tacaccagct
     2401 gcaacttgtt ggaataatga aataatccct agacctaaaa aactgataat ccaaatatct
     2461 tttactcgtc tcgtatatct taactggtca ggtgatataa ttgcaccaac tataacaaga
     2521 cctactacaa tattaagacc ttcaaccata ctcatagttg tttgaggagt ataattataa
     2581 atagcttcaa ctccataagt agaaaaagct ttaaaaactc cccacaccat taataccata
     2641 agtggtggta taactaagta attaaattta gccatagctt taaagccata cagtgcaaaa
     2701 acaaccataa ataatccaag tataactgta actaatgtta ggtttacact tgggaatatc
     2761 ttttgtactg ataatcctgc aacccctgct tgtacaccaa accatccaaa actagttata
     2821 ccaagtaata gagatataag atactttcct cctatctctc caaatgcccc cttagctaat
     2881 acatacgtat ttagcccttc tttgtaacct atataacctt gtatcataat aattgcaact
     2941 aatattaagt tccctataat tgatgtaata atcatattag aaagagtcat tcctaaaatt
     3001 aaagttcccc caaccattag ttcagatata gctatgttag aaccaagcat attaaacaat
     3061 ggagctaacc atccaactct ttcctctttt ggaactggat ttaaaactaa ttcttctctc
     3121 tcttgcaata ttatccctcc ctttctttat aaaagaataa tttctacttt tttctacttt
     3181 tttttcacta ttaatagaat ttttctttat ataattgtaa ttctattcta taaaaaagta
     3241 taatatctag tttgttatca atttgatata atttaagtta taaatagata aatagtccaa
     3301 atttattatt ttaatttaat ctattaagtc atatattaga cttatttcaa atttgttata
     3361 tattctttaa ttaaaatcgt gtacttatta caaaagtagt ttctaaaaca atcaaattta
     3421 tgtaaaattt ttaaacatag tattttaatt atatttgctg agaatacaag gataatttat
     3481 gaaaatatat agagagaata tataaaataa ttctaataaa aaaaagtctg aatataaata
     3541 tactttatat ccagactttt tacattttag tttcttgcaa ctacatatta ttcattatct
     3601 tttatatcaa cttttatgct taattcttct aattgctcat cttcagccaa tgtaggagca
     3661 tttgtcatag ggcaaacagc attttgattt ttagggaatg caataacttc tcttatatta
     3721 tcagttcctg caagtaacat aacaagtcta tcaagaccaa aagctagacc agcatgagga
     3781 ggagtaccat atttaaatgc ttctaataaa taaccaaact tttcattagc tctttcctct
     3841 gtgaaaccaa gtgctttaaa cattcttgat tgaacatcag aatttgatat tctaacactt
     3901 cccccaccta tttcataacc attcaataca atatcataag ctttcgctct taaagatgcc
     3961 ttatcacctt cttctaactt gtctaaatct tcatccatag gtgatgtaaa tgggtgatgc
     4021 attgctgaga atgtgcctgt ttcttcatct tcttcaaaaa ctgggaactc agttacccat
     4081 agtaagttat atacattttt atccaataga ttaagcctat ttgctacttc aagtctaact
     4141 tgtcctaaag catcaaatac tattgagttt ttatcagcta caaataaaag taaatctcct
     4201 acttcagcat tcattcttgt aagtatagcg ttcatttctt cttcattaaa gaattttgct
     4261 attggagaag ttactccttc ttgtcctact ttcatccaag caagaccttt tgctctatat
     4321 gtcttagcat gttcttctag tgatgatatt tgttttctag taaagtcgtc agctttgcct
     4381 tttacgttta tacctctaac actttttcct ggttgtgtgg catcagcaaa cactttaaat
     4441 ccacagttac aaacaacatc agatatatct gttaactcat atccaaatct agtatctggt
     4501 ttatctgaac catatttaga catagcttct gcataagtca tcttaggaag tggaagtgtc
     4561 aattctacat ctaaaacttc tttaaatatc gcttcaagca aaccttcaat catactcata
     4621 acatcttctt gctcaacaaa agacatttca cagtctattt gtgtaaactc tggttgtcta
     4681 tctgctctta agtcttcatc tctaaagcat ttaactattt ggaaatatct atccatgcca
     4741 cttaccatca ataattgctt atataattgt ggtgattgtg gtaatgcata aaattttcct
     4801 ggattaactc tacttggtac tagataatct cttgctcctt ctggtgttgg agctattaaa
     4861 aatggtgttt ctatttcgca aaatctatta tttgataagt aatttcttgt aatattcatt
     4921 actttatgtc ttaattttaa atttctttgc atagatggtt ttcttaaatc taagtatcta
     4981 tattttaatc ttagtgcttc agaaacatca tcatcatctt ttatataaat aggcggagtt
     5041 tctgacttgt ttaaaagtct aagttcagtt gcaaatattt ctatatcacc agttggcata
     5101 ttcgggtttt tagattgtct ttcgtatact tttcctttta cagctattac aaactcagcc
     5161 cctaattttt ccgctttttc aaaagcttct ttatctacat ctgtatcaaa aactatttga
     5221 cataatccac ttgtatctct taaatcaaca aatacaagac cacctaaatt tctttttttc
     5281 tgtacccatc ccataagtac aacttcttca tttatgtttt tttctctcaa ctctccacag
     5341 tagtgagttc tctttaaacc ctttaaagtt tccaagatac tacctccatt ttatttaatt
     5401 ttaatgctat atatttatct aatacacaaa acatcatact ttcttatttt acagcttttg
     5461 ctttaattct tcaactaatg tacttaattt tacagtagtt tgctctgatg ttttcatatt
     5521 ttttaatgtc gcagtgtcat ttttaagttc atcgtctcca attactattg taaattttga
     5581 atttatttta tctgaatatt taaattgtgc tttaacactt ctctctatat ggtctttatc
     5641 tgcacttata tgattttgtc tcaagtcttt taacaattta aagcttcttg tatttgcttc
     5701 ttcacctata gtcactataa atatatccgt agattgaggg ttttctattt caattccatt
     5761 accttcaagt gtaagtaaaa gtctttcaat tcctagtcca aacccaattc cacttatacc
     5821 ttttggccct cctagttgtt caacaagacc atcatatctt cctccaccac atacagtact
     5881 ttgagctcct atatcattag atataatttc aaaagcagtt tttctatagt aatctaagcc
     5941 tctaactatt gttttatcaa ttacaaagtt tatattcatt tcctttaagt attcttgaag
     6001 tttatcaaag tgagatttac aatcatcaca taaatggtct gcaataaatg gtatatcttt
     6061 gatattttct ttacatgtag gatttttaca gtctataacc ctcattggat ttttttctct
     6121 tctctcattg caagtatcac acaatatatc agcttttgag tcaagatatt cttttaataa
     6181 agcattgtac tcttttctgc aaactggaca accaactgaa tttatactca ctaccaaatc
     6241 atttaatcca acttcattaa aaaattgaac agctagtgcg attatttcag catccattga
     6301 aggagtatca cttccaagtg cttctattcc aaattggtga aattgtcttt gtctaccagc
     6361 ttgtggtctt tcatatctaa aacaaggtgt cacataaaat aattttgttg gttgagtatc
     6421 tgcatataat ttattttcta taaatgctct tactacacca gctgtacctt ctggctttag
     6481 ggtaatttct ctatcacctt tatctttaaa agaatacatt tctttttgaa ctatatctgt
     6541 agtatctcca acacttctct tgaaaagttc tgtatgttca aatataggtg ttaccatttc
     6601 ttcatatcca tataaagcac atatttctct aaatttattt tctacataac gccatttgta
     6661 agcctctttt ggagttatgt ctttagttcc tctaggagct ttagtcaaca tttttatata
     6721 cctcttcttt cttccttttt atcatttcat ctactctttc aatgtattgt tcaacatttt
     6781 ttacattttc tactctttca attctatctt catctacata aacaaatttc gatatagcac
     6841 ctgcacctaa tgcataatta ctttgttttt cttccataat ctgtatatta taaatgcact
     6901 catatccttc tttagcataa cctatatttt ctaaatttcc caacatatgt ttttgtctgt
     6961 acatataata tggatttaat cccatatctt ttgcatattt cattgataaa tctatcattt
     7021 ttaccatttc ttcgtattgt gttagatgtt ctttatacct atccatattt atatttaagt
     7081 tggatgctct ctttatggct aatgtatgaa ctgtaaggct ttcaggagaa agttttttta
     7141 tctcctccaa tgtgttcttt accatattta agtcttcatc tataagacct aaaattatat
     7201 ccatatttat attatcaaat cccattctac gagccatatt aaagcaatct actaaatcag
     7261 atactctatg ctctcttcca atctttacta aggtctcatc attcatagtt tgaggattta
     7321 tactaattct actaacattg tgttttttta acactcttag tttttgttct gtaatagtgt
     7381 caggtctacc tgcttctaca gtaaattctt taattttact taaatctaat tcattaaata
     7441 atgaatttat aagcaaatcc aattcttcct catcaagtgt agttggagtt ccaccaccaa
     7501 tatatagagt ttcaacttct ttcttagtct ccttaataac cttggcaaac cccttaattt
     7561 cttcaatcaa tttataaaca tattctcttt ttaaatgtcc aaattgtttt aatgaatttg
     7621 aaggaaatga acaatataca catctagttg gacaaaatgg aatacttaca taaagagaaa
     7681 ttttattttt atctatagga tacataaata gtcgctctct tttagctatt tcaagagcta
     7741 aatctatttt ctcatctatt ataaaataat tatcttttag aatggttctt atgctattat
     7801 catctagctt tttatccatt aaattgtgga caattttgac tggtctaata ccagtcaaaa
     7861 ttccccatgg tacataagaa ttaaatactt gtttaagcac tacaaacatg ctttttttga
     7921 tattttcttt acacagcttt ttaaagttcc tcttactttc attatctaag ctattaagaa
     7981 attcttcaat cgttttgcta ctatcaaatc catctaaaaa ttcaagattc atattttcta
     8041 tattttgtat tgactcgtac tttaagtttc cattttcata gtattcagta cttgagaata
     8101 atacaccatt ctcataaatc aatctattta ttaaatgctt tgactccata ttattattag
     8161 ctttagagtc attaaactta aattcactgg taaacaattt tataagttct gctacttcat
     8221 acttataatc atgtcctttt aaaaaaacat acaacatcaa cttaactcct tctatttaac
     8281 agtagaagaa ccccctcact aatatagtta ttaaatattt tatacttttt taaatactaa
     8341 tataaaatta catgtaagga tttgtcttct tttcaacccc tagagtacta gttggtccat
     8401 gtccaggata tactctaaca gcatcttcat atttacataa tttcttaagt gatttttcca
     8461 tttggttaaa attcccacta tataaatcag ttctaccaat acttccagca aataaagtat
     8521 ctcctgtgaa catttcatct tcaactctta tacacataca tccttgagta tgtcctggag
     8581 tatgcaagaa agtcattttt aattcaccaa gttcaagctt gtctttatca ttcacataga
     8641 tgtctgcatc aaactcttgc gctccacatc tcattgtata acttagattt ttattcttgt
     8701 catttaataa ttctttttca gcaatatgtg caactacctt agcaccagtt ttttctttaa
     8761 tatccttaac atatcctata tggtctccat gcccatgagt aaggataata tattcaatat
     8821 ttaattcatt ttcttttaca aaacttaatg tctctactaa acttccacct gggtctataa
     8881 ctgcacattt tttagtcttt tcatcaccta atacataagt atttactcca aaataactat
     8941 ctacaaattt ttttataatc attaaagttt tcccccttaa aaagatttac ttgagtctaa
     9001 tagtattgtt actggaccat cattagttaa ctctaccatc atatgagctg caaattgacc
     9061 tgtgccaact gtcacacctt tatcctttgc tttttttaca aattcttcat aaaggttatt
     9121 tgcaagttct ggtctagctg cattactaaa tccaggtctt cttccttttc tacaatctcc
     9181 atataaagta aattgagata ctactaaaag ttctccacct atatccatta gagataaatt
     9241 catcttgtca ttttcatctt caaaaattct cagattcaat attttgtcaa tcatataatc
     9301 tacatcactt gatttatcat catgagtcac tcctaacaat actagtaacc ccttatttat
     9361 ttgacctata gtgttttcat ctactgtaac tttagatgaa gatactcttt gtactactgc
     9421 tctcatagct tcctccctat tattttaact ctaatttgtc attctataaa tactttctac
     9481 accaggaatt gcttttaatt ttttcattag aatttttaat tcctctgtat cattaacttc
     9541 tacaagtaag ttgatactta caatactatc cttacctttt cttgcattta ttccatttaa
     9601 actaagttta tccattgcaa caatatgagt tatttcatta acaattcctc ttctgtcaat
     9661 agcttttatt tgaatttccg cttcaaactt accttttcct gctgaactcc atgaaacctc
     9721 taccaatcta ttttggaaga aatcaccatt attgtttgca ttcgggcaat cagctctgtg
     9781 tatggctact cccctaccct tagtaatata tcctacaatt tcatctcctg gaagaggatt
     9841 acaacactta gcaaatctta ctaagatatt gtcaagccct tttactataa ttccttgtga
     9901 tgcatttttc tttctctttt tactgtattc tgtttcactt atattatgtt tatttaaatc
     9961 ttcttcttgt atctgttgtt cctttttagc ttttttaacc tcttttaaat ataggtcttt
    10021 tactttagat accacttgag atgacattat accaccataa cctacagtgg caattaagtc
    10081 ctccacactt ggctgattaa atttcttagc aactaatgcc atatattttt caacaagagg
    10141 gtctttttgt ggtatatcat gcttcttaaa ttctctttct aatattgtat ttccacggtc
    10201 aacattttct tctcttcttt ccttcttgaa aaattgtcta attctacttt ttgcatttgg
    10261 ggtcttcact atattaagcc aatccctact tggtccatta gaattagatg atgtaagcac
    10321 ctcgacaata ttaccatttt gaagcttata atcaatagga actattcttc catcaatttt
    10381 agctcctaca catttattac ctacatttgt atgaacccta taggcaaaat ctataggtgt
    10441 tgattctgct ggtaattcaa ttacgtctcc ttttggtgta aatacataaa cttgactatt
    10501 aaatacatct tctttaagtg catccataaa ctcttgtggg tcttttaaat ctttttccca
    10561 ctccatcatc tgtctcagcc attgaagttt ttcttcaacc tttgactctt tgctagagat
    10621 accttcttta tatttccaat gggctgctat accattttca gctatattat gcatctcatg
    10681 agttcttatt tgtatctcta aaggttctcc atcaggacct acaacagtgg tgtgtaatga
    10741 ttgatacata tttggttttg gcattgctat ataatcttta aatcttcctg gaataggtct
    10801 ccataatgta tgaactatac caagtactgc atagcaatcc tttacattat caaccaatat
    10861 ccttactgct gttaaatcaa aaatctcttc aaaagtctta tgtttttttt gcatctttct
    10921 atatatactg taaaaatgct ttggtctacc atatacatca catggtatac ttaaatcttt
    10981 aaacttatct ttaagaagtt caacaattcc ttgaatataa tcctctcttt gacttctctt
    11041 cattgatact ctactaacta aatcataata cccttctggg tccataaatc ttaatgctct
    11101 gtcttctagt tcccacttta tcttggaaat tcctaatcta tgagctatac caccataaat
    11161 ttcaagtgtc tcttttgctt tatactttgc tttttctgga ggcatgtatt ttaatgttct
    11221 catgttgtgc aatctatcag caagttttat aagtataacc cttatatcct tcgccattgc
    11281 caaaaacatt tttcttaaat tttcagattg agtttcttct ttcgaacgat attttatttg
    11341 accaagttta gttactccat caaccaaatc cgctacttct ttgccaaatt ctttttttat
    11401 gtcttcatag gtatattctg tatcttctac aacatcatga agtaaacccg ctgttatagt
    11461 ttcaatatct aattccatct ctattaaaat atttgcaact gctaatggat gaataaagta
    11521 tggttcccca gattttctat attgtccttc atgcgctttt tttgcaagat tataggcttt
    11581 ttcaaccatt tcaaaatttg cattaggtgc ataaactttt attttttcaa ttatttcttg
    11641 taattcttta tcatgcatat aaccacctat tttgtataaa attttaatat atatgaaaat
    11701 agttggtgtt ataccaacta ttataaaagt actagtattt tataagagaa ttaacatcat
    11761 agttagacaa tttttctctc ccatttaaga attttagctc aatcaaaaat tgcatagaaa
    11821 ctacttctcc acctaatttt tctacaagct tagcagcagc ttccatagtt ccaccagtag
    11881 ccaataaatc atctactatc gctacttttt gacccttttt aattgcatcc ttatgtatct
    11941 ctaaagtatc tgtaccatac tctaatccat actcataact ctcaacttca cctggcaact
    12001 ttccaggttt tctaattgga acaaatccta ctcctaaagc atatgctaca ggtgtaccca
    12061 tcaaaaatcc tcttgcttct ggtcctatta ctaaatctac atctttatct tttaattctt
    12121 caactataga atccactgca tatttaaatg catctccatc tttaaataga gttgtcacat
    12181 ctttaaaatc tatacctggc tttgggaaat catcaatatt ccttataaag ttttttaaat
    12241 ccatgggaaa cgcctcctaa taactgctat tgtattgttt ttctaaattt tttagattat
    12301 tgagtattaa actctcattt aagtctaatt ttttctgtgg ttttggcaat attcttatga
    12361 gtacagaatt tttttcataa tttaaattaa agtcaagtaa atttaattct ctaaatacat
    12421 ttaatatcgt aaaaaacttt aatggaatta cattaaaagt tccccttata tttaccatat
    12481 ccaaatcaat ttcttttttt atgagtgctt gtttataaat tgttataaat tcatctcttg
    12541 atggaactat attactaact acattcttta aatataatag gtctgtttta ttataatatt
    12601 ttataatttc actttcttct cttttattct catatatata cgaatattcg cctttattgt
    12661 ataaataatc ataaagaata atattattat atctttttaa gtctatttta tcaatattag
    12721 gagaaaatat aagctctaat ttatcagtat ttttgctaac atcttcaata atattgtaat
    12781 ttatgttaaa atccaaatct ataagtgata tatctgacat tgctctataa aatccattta
    12841 tagaattaga tattattagt gtatcttttt caaaataatc aaatatattt atatctgtat
    12901 ttccatcaaa taattcaaaa tcagatacag atatattata caggctttga gtatcttttg
    12961 gtattatttt tgacattaat ttcaatgaaa gtttgtcatt acttgcactt ttaggtcgag
    13021 ccaatcgtat atctttaagt aaaaattgta ctttcctatt tcccatatag ttattttcat
    13081 ctaattggaa caatacatct actttgtctc ctactttata cattgatttt aaatgactca
    13141 tattaaatcc tatacattca tagcttttct ctttttctat gcttaatttc agatgttgtt
    13201 gattcttacc tatgactttc aaatctttta aaatataatt tctaactata aatctaggat
    13261 taggattatt taatccaaag ggttctaatt tatgtaattc ttctacaaga tttaaattta
    13321 ttacattttc ttgtaattca aactcaacat ttacattctc tatcatatca tcttcagtta
    13381 aattatagtc tgcaaattta tttatttcat ttgctaaaat ttctacattg tctgaatcca
    13441 aagatagccc tgctgcttgt tcatgaccac caaacttagt catcaagtct ttgcacttta
    13501 tgagagcttc aaatatatta aatcctttta ttgacctcgc tgaaccagta gcttccccat
    13561 tttctatacc tagaagaatt gttggtttgt agtatttttc agttaacttt gatgctacaa
    13621 ttcctattat tccatgttgc caaccctctt tagctaaaac caacactttg tcatcattgt
    13681 atctgctatt agactttagc atttcttcag cttcatggta catcttagct tctatcatct
    13741 gtctttcatt atttttttct tccaatatac tagctatttc tactgcctct tcttgtgatt
    13801 gagtagtaaa tagctctact cctaagtaag aatatcccaa tcggcctgaa gcatttatcc
    13861 taggacctat tgcaaaacct atatgagaag aacctatctt atcactttct acaccacaaa
    13921 cttttataag ctcccttaat ccaatatttt tgccttcttt cattgatttt aaaccatttt
    13981 ttacaattat tctattttca tctataagtg gaacaatatc acaaatagtt gccagtgtta
    14041 ctatttccaa ataattatac atactagttt taaattcttc ttttggagtc aaggcttgaa
    14101 tcattttaaa tgctacacca catccacata gtgaatcaaa tggataatta caatcttctt
    14161 gttttggatt tattactgca aaggctgatg gaatttcact ttgacattca tgatggtccg
    14221 taattataac atctatgccc aaatcattcg ctgtattgac ctcagacact gatgtaattc
    14281 cacagtcaac acttataatc aagtcacatc ccctagagct tataagctta atagcatctt
    14341 catttatacc atacccttct tctagtctat taggaatgta gtaatcaaca ttgtaaccta
    14401 tactcttaaa atataagcac aagatagatg tagatgatac accatcaaca tcataatctc
    14461 cgtaaatcca aattttttcg ttcttctcaa ctgctaactt tattctatca acagcttttt
    14521 gcatatcttt cattaaaaaa gggtctctta aataatctaa agatggattc ataaatattt
    14581 ctgaatcttt ttcagtacat atccctctat tttttaaaat ttggcagatt tccggggaaa
    14641 tattgagttt tttgctaaat tcactttcct ctatcttacc tttatacttc aatatccatt
    14701 ttttcaaatt taattccccc taatcttttt tgtattttga cctaatactt cttataacta
    14761 agcaaaggcc tattataaag gatataaaga aacctgctat tgcagtaaat tttatccata
    14821 tattattttc tatatttgga gatgcaatta taagagaaga acctactata atagatgcca
    14881 acactaaact caaagatatt tgagatgtta actctattat acaattttct aacctcgtca
    14941 ttttaacatc ttcaatttgc atttttatat tatttctttc tatatttttt aaaattgctt
    15001 ttaattgctt tggaatagtt tttatgtcca gtaatatttc ttctacattt tgtcttgaac
    15061 ttaaaacaac atttttaggg ttaaatttac ttttataatg atgtttcata aactcttttc
    15121 ccatagaacc aaaagaaaaa tctgtgttta aagttctact tgttccttct aatgttataa
    15181 ctgtctttgc aagagttaca aattgtgcag gcatgactat tttgtactgt ctaaaaaatc
    15241 taaatagttc atttaatata tctgttacat ttattttttc tatagaaata tcatagtaat
    15301 aatgtattag ataaagtaaa tcctgtctaa gtcccttaat atcagcctct ccatttaatg
    15361 catccatttc tatcaataga tatattatct tgtcaacatt cttttctaca cctgctaatg
    15421 caatttcatt taattggttt aaagttttat tatcaataat tccaatcatt ccaaaatcta
    15481 tatatgctat cttattttta gcaactacga atatattacc tggatgtggg tctgcatgaa
    15541 aaaatccatg agataatacc tgtgtaaaaa atgacctaac tcctatttca acaatagttt
    15601 ttgtgttata acctagtctc cttatttttt ctacatcact taactttgta ccattaactt
    15661 tctccattac taaaattttc ttagtattgt attctgaata tactttaggt atatatactt
    15721 catcactatt tttaaatatc ctagagaatt tagttgcatt tatagcttca aatgtataat
    15781 caagttccct cattaattga gtttgaaatt cttcaatcat ttgtactaaa tctaaattaa
    15841 aatctttttt taaatctttt aaagtatttg caatagttct aagaatctct aaatcagatt
    15901 ttattatttt ctctatattt ggtctttgaa tttttacgat tacttcttct ccagttctaa
    15961 gtacaccttc ataaacttga ccaattgaag ctgctgctat tggttcttct ttaaattcta
    16021 agaatatttc ttctatactt aaacccactt cttctttaaa tatatcaata gctatatttg
    16081 aatcaaattt ctctacatca tctctgagtt ttgatatttc atcaattata tcctggtcta
    16141 acaagtcttt tcttgtactc aatatctgtc ctatttttat atatgtaggt cctagttctt
    16201 ctaaaactct ttttattctc tcaccagttg tcatattttt tatttcttct ggtgggtcaa
    16261 atagtggtat cttataggca attccttcta tatttagtct ttctacaata aaactaaaac
    16321 catactttat taaaacatat ccaatttctc tatatcgctt taaatttcta taacttattc
    16381 tcaaatcttt tcactcctaa gataggtata cccaaaaact tacaccttaa tttatcgtat
    16441 atgacattat ttcaccaatt gatattctaa tatcttatat cctctttaat atttaatttt
    16501 atcacatata ctaccaattt tattaaaatt atttaactca gttaattttt atttttgaca
    16561 ttttatacct ggtttaatca aatacttttt gaaaactaaa atatcaacaa aatctaataa
    16621 aataaattag tggttgcctc aaaacagatt taaatatgtt ttgaaacaac cactctttaa
    16681 actaatattt ttgattttgt gctgcctcta aataccattc ataagattca tcaatatatc
    16741 ttttatcttc ttctgtaagc tctctaacaa ccttaccagg agaacccatt atcaaaacac
    16801 ctggtggaaa cttcttatta gatgtaatta atgtacctgc tcctattaga gtatattctc
    16861 ctatttcagc attatccaat attattgaac ccataccaat taacacatta tcactaattt
    16921 tacaaccatg aactatcgac ctatgcccaa ctgttacatt gttaccaatt atagtttctg
    16981 tatctccatg cactatagaa caatcttgta tattagtatt ttctccaata gttattggtc
    17041 cttcatcacc cctaactact gcattgtacc atatacttga atctttgcct attttaacat
    17101 ttccaattac atccgctgat tttgctacaa atacactttc gtcaattaaa ggtttatctt
    17161 ctaaataatc tctaatcata tataaacaac tcctttaaaa tataaattta gttggttgct
    17221 ccttctaaca ttaacttagc ttgtatcatt atagagcact ctgtacgttt tctttgcatt
    17281 tcacagccca attcatatgg ttttgatatg tcattattaa agtttatcgc atttgcatga
    17341 catccacctg aacagtaaaa cttattccaa cattgtttac actcttcttt ggtatataca
    17401 tgagcttctc taaacatatt ttttaaattt tctggaagta ctatttcttc atcaaatata
    17461 tttgccattt taaattcttc atttccaaca aattgatgac atggatagat atctccattt
    17521 ggagttacag aaagatactc atttccagct ccacatccag ttattctttt tattacacat
    17581 ggaccttgat ttaggtctat cataaaatgg aaaaatttaa atccatctcc ttgtaattgt
    17641 ctatctgcat actctacagc aaatttttcg tactcttcaa atatcttagg taaatcttct
    17701 tctctaagtg catatggatt gctctcatct ccaacgactg gctctacaga ggttaattta
    17761 aatcctaaat ctgcaaaatg cataacatct tttgaaaaat ctaaattatc tcttgtaaaa
    17821 gtacctctta tataataata cttatctttc gctctttttt ccacaagttt cttaaatcgt
    17881 ggtaatgtaa tatcataact tcctttatca tttaaagttg gtctcatatt atcattaact
    17941 tcttttcttc catctaaact taaaactaca ttatgcatat tttcatttat ataatctata
    18001 atctcatcat ttaacagtac accatttgta gttattgtaa atcttatatt tttattataa
    18061 tctttttcta cacttcttcc ataatcaacc agttgtttta caacctcaaa gttcatcaaa
    18121 ggttctccac caaagaaatc tatctctagg ttctttctac ttcctgaatt agcaatcaaa
    18181 tagtcaattg ctgccttacc aacttcaaaa ctcataagtt ctttttctcc tccaaaatca
    18241 ccttgagctg caaaacagta tttacatttt aagttacaat catgagctac atttaaacat
    18301 aaagctttta ctacaggctc tctatgtaca aaacttggat gatattgata tgtatcttct
    18361 gtatataaaa gaccttcttt ttccaaattc aatatttctt catatgcttc atttattttt
    18421 tcttcttgat attttgattt taatatctct aatatttctt ctttactttt ttctttataa
    18481 aaatctaaca aatcgtatgc aacatcatct aacacatgaa cagctccacc attcacatct
    18541 aaaacgatat tatatccatt catagaaaat ttatgtatca tactcatacc ctacctccta
    18601 aattactatc tatactaaag ttttttatta ttttgattaa actttaccct tcattatatc
    18661 ataagtttac ataaaataca tacaatacaa aaatgactta ctctccgtaa gtcatttcat
    18721 taaatacact atatccccct aaattggcgc actaatatat taccaaatag atattaggct
    18781 cctataaaat aaataaaata aattttgtat ctgattttat attgttttaa tcttattaaa
    18841 atataataat ttcttttttt ctgataattt tgtataaata agtgcttgta tttaatttgt
    18901 cttctttaaa ttgctgattt ctttgggtac tttaggctga tgaataatcc aaggacaggt
    18961 actattagct gacaaaatag cagtactaag tgccaatgag ctagcaaact tcataaatct
    19021 tacaataaac tttttcataa cttaactctc cctcatacat attttactta tattttagct
    19081 tttaacttac ctaatactaa catcacaaaa atccaaaaca tggcaaatgc gctatatttg
    19141 aaataatcta caaatatact caaagaaaga cttaaaattg ttatcaataa cactactgta
    19201 gatataaata tagcaatttt cttataaacc aacttctccc tatcacttat agggttgctc
    19261 ctatgttcta ttgggcatag aacacaaatt ccaacccaac taacacttgc aataatcata
    19321 ataatatttt taaataagtc tatatttaga ttttctataa ttaatattgt ggataaaatt
    19381 ataaaaataa aggttaataa gcatctaaaa tagttgtctg catgatatcc tcctgaaaat
    19441 tgtcttattg gacaataaca cattaaaaaa aacaatacat atgtaaattt gccaaataaa
    19501 aaacctataa atagcatggt ggttatatta actatgaaag ctattaatat ttcaaatcca
    19561 taagaataga ctttagattc attattatct atcatattat tacaaattaa aacagatgtc
    19621 attttttcag catatctttt aaacattttt acctccaaaa aaattttaca tatatagagt
    19681 acattctttt ttgagaaaat tcaatatact ttacaaaact agttgttaaa aatgtaaaac
    19741 tggtcaaaaa aagacctata taattttctt cttattattt atataacaaa acaattataa
    19801 taaaacaatt ttatcaagct ttatatgcat aaaaaggcga tatcaaaata gagataaatc
    19861 tttttttcat tgttaaaagg ctaaaaatat attttatttc atataaaaag agtacctcac
    19921 aaactaaaaa gttcattttg agatactcat tttatttatt acaaaatatc aatttttatt
    19981 tatttgcata tattttctat atattttggc cttttaactt tgtttttgtt ttacatactg
    20041 tagtgctttt ctattctgtt ttactgcttc aagacaaact ttttcggttt gattatcaac
    20101 aaattctaaa gctctataat tttgctttac tgcttctaaa catatttttt cggtttgatt
    20161 atttatatat ttcaacattc tataatcttg ttttactact tttagacaaa cttcttctgt
    20221 ttgttctttc acatatttaa gcgcttcata actttgatta actgcttcta aacaaatctc
    20281 ttctgtttgt tcctttacat aagctaaagc attatagttt tctcttactg cctctaagca
    20341 cattaattct gtctgacttt ctacatattt aagaattttt ccattttgcc ttactgcttc
    20401 tataaatatt ctttctgttg gtacctttac ataacttaat atttctttta tttcaagttc
    20461 ttcaaatgaa cctcttactg cttctataca tatatcttca gtctgttttt ttacgtattg
    20521 caatactttt ccattttgtc taactgactc caagcaaatt tcttctgttt gctcttttac
    20581 gtactgtagt gctcttccat caattcttac tgcttgcaaa caaatctctt ctgtttgctt
    20641 atttacatac tgtagagcca tataacttct ttttactgct tccatacata ttttttctgt
    20701 ctgttcattt acataataca atgcaaagtc attctgtttt actgcttcta tacatatttc
    20761 ttctgtttgc tcttttacac aagatagtgc actgtagttt tcttttacag cttctaaaca
    20821 tatactatta ctttgttcct ttacataagc taaagatttt ccgtcttttt ttacagcttc
    20881 taaacatata gctggagttt gctcttttat tccttttaat atctctgcaa catcattttt
    20941 agatgccctt tctataaatg ctagacatac atcttctgtc tgctcttcca caaatttaaa
    21001 tacttccatg acatcataag ttgatgcatt ttttatagcc tcaattagta cattttctgt
    21061 cttattattt atgtagtaca aagctctgta attctgtttt actgcttcta tacaaatatc
    21121 ctcattttga tttgttatat acttaagagc tttatatgtc tgttttactg cttccataca
    21181 tactttataa gtcttgtcct ttacaaactc aattatagta ccatcctgct ttactgctag
    21241 cagacacatc tcttctgttt ggttttctat gtcttttaac tctctgtaat catattttac
    21301 tctgcttaaa tcatttaaat gtactttgtt atcttctttt gaatcgtcct cattattttc
    21361 tatatcatac tcaaattctt catcatctaa atagtcattt ttttctggta aaaatataga
    21421 tttaatttta ttctttatat ccataaaatt atcctccttt aatatgattc tatcattaaa
    21481 gtaaatcgtt tttaagcact tatttaatta attttatatt aagtttcttt aaaagtctat
    21541 tcattgttga tagattatat tttatattaa tacttttgtc ttatatatgt ggggtaataa
    21601 agttaatagt ggacattttc catcccatta gtatacacta tttatattta ttactcattt
    21661 atagtgattt
//
LOCUS       DAEMAE010000047        21294 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEMAE010000047 DAEMAE010000000
VERSION     DAEMAE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.048"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            63..1715
                     /locus_tag="KPH93_001422"
     CDS             63..1715
                     /locus_tag="KPH93_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1688251.1"
                     /translation="MKICKKLLTMGISLLLVISAFPIVSNGLSSIDNINGKDKYETSA
                     IIADRQNYTKAILINVDNSLADGLSASGLAGVENAPILLTKKDDIPDSTLKRLNNVKK
                     VYIIGGNNSIGSKVDNLLKKKHIEVERIEGKDRLSTSYKVSDKVLELKGTSGNILVAN
                     GFKGEADAISAASVAFKNAIPVLLTNGSDMPELKIKGDKIFAFGSTNTMSNQLVEKLG
                     ATRLGGIDRYETNKKIVQQFYGDAKEFYVASGSDLVYPLIGSTLAKSKPIVLVGNGSN
                     KSILKGATKITSIGNIEASIVTQCLNVTNNIGDTNTGVVKTNTNKEYPIKGMLAKLGL
                     NTTGKIGWDLNYGGNGNGVELRADGKYYYINRGNTALGAYAAALAGEKYHSLDFGDLD
                     PIEVIADKEEISYEKAQKEIVVIRNFLNSFDWQNASDLEKATRAGKLVTEADYVMGNY
                     NIYTNLLEKKSVCEGFAKSFYVLTRLMGMDSLYQEDGNLNHAWNYVKIDGKWYEFDGT
                     EAGSYKNLGIKVEFNSSKLEEATKQMPKYYDAKALSVLGFNQ"
     gene            2142..2663
                     /locus_tag="KPH93_001423"
     CDS             2142..2663
                     /locus_tag="KPH93_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688252.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIEENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            2697..3473
                     /locus_tag="KPH93_001424"
     CDS             2697..3473
                     /locus_tag="KPH93_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1688253.1"
                     /translation="MKFHVFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTGMPHVRKETFYNMYRTYMMSYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            3815..4768
                     /locus_tag="KPH93_001425"
     CDS             3815..4768
                     /locus_tag="KPH93_001425"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG1688254.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            4786..5805
                     /locus_tag="KPH93_001426"
     CDS             4786..5805
                     /locus_tag="KPH93_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG1688255.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            5836..6087
                     /locus_tag="KPH93_001427"
     CDS             5836..6087
                     /locus_tag="KPH93_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688256.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFNIPNLDIHEIITKFNK"
     gene            6369..8174
                     /gene="lepA"
                     /locus_tag="KPH93_001428"
     CDS             6369..8174
                     /gene="lepA"
                     /locus_tag="KPH93_001428"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG1688257.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            8325..9662
                     /locus_tag="KPH93_001429"
     CDS             8325..9662
                     /locus_tag="KPH93_001429"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG1688258.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERIPNDWD
                     ITTNARPEAVVELFKKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVIQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            9894..11207
                     /locus_tag="KPH93_001430"
     CDS             9894..11207
                     /locus_tag="KPH93_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1688259.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQRPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            11379..12554
                     /locus_tag="KPH93_001431"
     CDS             11379..12554
                     /locus_tag="KPH93_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG1688260.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            12657..13676
                     /gene="hrcA"
                     /locus_tag="KPH93_001432"
     CDS             12657..13676
                     /gene="hrcA"
                     /locus_tag="KPH93_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG1688261.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            13706..14326
                     /gene="grpE"
                     /locus_tag="KPH93_001433"
     CDS             13706..14326
                     /gene="grpE"
                     /locus_tag="KPH93_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG1688262.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACENKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESIDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            14418..16265
                     /gene="dnaK"
                     /locus_tag="KPH93_001434"
     CDS             14418..16265
                     /gene="dnaK"
                     /locus_tag="KPH93_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG1688263.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            16476..17630
                     /gene="dnaJ"
                     /locus_tag="KPH93_001435"
     CDS             16476..17630
                     /gene="dnaJ"
                     /locus_tag="KPH93_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG1688264.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRTRRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            18595..19530
                     /locus_tag="KPH93_001436"
     CDS             18595..19530
                     /locus_tag="KPH93_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG1688265.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            19566..20939
                     /locus_tag="KPH93_001437"
     CDS             19566..20939
                     /locus_tag="KPH93_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1688266.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGRWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(21127..>21294)
                     /locus_tag="KPH93_001438"
     CDS             complement(21127..>21294)
                     /locus_tag="KPH93_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688267.1"
                     /translation="DLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYL
                     SVKSYFLYKKI"
BASE COUNT         8421 a         2361 c         3904 g         6608 t
ORIGIN      
        1 tttttttcac cttatagtat atcatttaag tggaactata atatctggga ggtatttata
       61 ttatgaaaat atgtaaaaaa ttattaacta tgggaatttc attattgcta gtgatttctg
      121 cttttccaat agtatcaaat ggacttagtt cgattgataa tataaatggt aaagataagt
      181 atgaaacttc agccattatt gcagataggc agaattatac taaagctatc cttatcaatg
      241 tggataatag tttagcagat ggattatcag caagtggatt ggctggagtt gaaaatgcac
      301 caatattatt aactaagaaa gatgatatac cagattcaac attaaaaaga cttaataatg
      361 ttaaaaaagt gtacataata ggaggaaata attctatagg aagcaaagta gataacttat
      421 taaagaaaaa acatattgaa gttgaaagaa ttgaaggaaa agatagactt agtacaagtt
      481 ataaagtaag tgacaaggtt ttagagttaa aaggtacttc aggtaatata ctagtagcaa
      541 atgggtttaa aggtgaagct gatgctataa gtgcagcctc agttgcattt aaaaatgcga
      601 tacccgtatt acttactaat ggttcagata tgcctgaact taaaattaaa ggagataaaa
      661 tatttgcatt tggttcaaca aatactatga gtaatcaact tgtagaaaaa ttaggagcaa
      721 ctagattagg tggaattgat aggtatgaga ctaataaaaa gatagttcaa cagttttatg
      781 gtgatgccaa agaattttat gtagctagtg gtagtgactt agtttatcca ttaattggct
      841 caactttagc taaaagtaaa ccgattgtat tagttggaaa tggaagtaac aaaagtatac
      901 ttaaaggtgc tactaaaata acatcaatag gaaatattga ggcttctata gtaacacaat
      961 gtctaaatgt aactaataat ataggagata ctaatacagg cgtagtcaaa accaatacaa
     1021 ataaggagta tcctatcaaa ggtatgttag caaagcttgg tcttaacaca actggtaaaa
     1081 ttgggtggga ccttaattat ggaggaaatg gaaatggagt tgaacttcga gctgatggaa
     1141 aatattacta tataaatagg ggtaatacag cgttaggtgc atatgctgct gcattagcag
     1201 gtgaaaagta tcatagtctg gactttggag atcttgaccc tatagaagta atagcagata
     1261 aagaagagat ttcttatgaa aaagctcaaa aagaaattgt agtaatcagg aattttttaa
     1321 atagttttga ttggcaaaat gcttctgact tggaaaaggc tactagagct ggaaagttgg
     1381 tcacagaggc agactatgtc atgggaaatt ataatatata tactaatctc cttgaaaaga
     1441 aaagtgtatg tgagggtttt gcaaagagct tttatgtatt gacaagacta atgggaatgg
     1501 attcactata tcaagaggat ggtaatttaa atcatgcttg gaattatgta aaaattgatg
     1561 gtaaatggta tgaatttgat gggactgaag ctggatcata taaaaacctg ggaattaaag
     1621 tagaatttaa ttcttctaaa cttgaagagg caacaaaaca aatgcctaag tactatgacg
     1681 cgaaggcgtt atctgtatta ggatttaatc aataaaagaa agactcttta aagagtcttt
     1741 cttttatttt aaatattcca aaattatttt taaatttgag taattactgt tgactatttg
     1801 atagttttac ataatgtact taaatggcat agaaaattta tttatatatt tttataataa
     1861 acagaaaatg aaaatagcaa tacaaatgtt attccaaaag aaactttaag tgttctggaa
     1921 ttgaataaac tgaaattaaa ttacattggt atatatgata ataaatacac caaacaatat
     1981 aactaatatg ttggaagatt atatgcaatt aatgtcagtg tttatataga atagcatttg
     2041 tagcatttat tataattgta attactattt atatcataga cttgaatagt ctaaaaaaat
     2101 agactataat atttttatag actaaagaaa gggctgataa gatggacata gcaaaactaa
     2161 ttttaaatcc tgcaaggctt cgtattttac agtatattcg acttcatagc agtgttcgca
     2221 catctgatat tgtaaagtat ttaaatgata ttcctcgtgc aacagtttat caccatgtaa
     2281 aaattttaga agagaacaat atgattgaag taattgaaga aaatcgtgtg cgtggtacta
     2341 ttgaaaaggt ttatgcacta aaggaatata caacatctat ggaaggagag accttcgttg
     2401 cactttctac agcattttat gtaggtctga tgcaggaaat gaatgaatac tttagcagaa
     2461 aagagcaaga ccataaaaag gataatgtat ttttctcttc agcattacta tatatgagtg
     2521 ataatgaata tgaaaatctt cttaaaagta tagctgattt attgaaacca tatatagagc
     2581 aaaagcctaa atcaaatcta aaacttagaa aactttcaat tatatcttct ccacctgtag
     2641 ctgataatga taaaacaaaa taacatgcac cccattttta taaaaggagg ttatgtatga
     2701 aatttcatgt gtttggaaat aagaacagac cacatattat gttgattcat gggggaggga
     2761 atgcatggtg gaactacttg cgacaagcag aagttttgtc taacaactat catgtgatac
     2821 ttcctacatt ggatggtcat ggagaagaat atgaaacaac atacatttct acagaggata
     2881 cagcagataa gttgatatca tatatagatg aaaattgcag tggtcatctt ttcgctttat
     2941 gtggggtatc acttggtggt caaattgtaa tggaactttt atctcgaaaa cctaatatta
     3001 ctaaaaaagc aattattgat ggtagtattt gctacccaag acctcttatg gcaaggtttt
     3061 gtattgctgg tattcgattt tttggaggac ttttatttag tgagaaggct tgtcgctttc
     3121 aaattgctat gatgccaaaa ttgttacctg aaaatatgca atatccagat gaaataaaag
     3181 catattatat gacaggtatg ccacatgtaa gaaaagaaac attctataat atgtatcgta
     3241 cttacatgat gagctacaca cttaaagaaa atgttaaaaa aaccacagca caagtaatgt
     3301 attggtatgg agaaaaagaa atgaagtgcg ttaaaaaatc tgcaaaaatg tttcagtctt
     3361 atgtaccctc ttgtgagatt tatgaagcta agggctataa tcatgggtat ttatcgcttt
     3421 atcttccaaa tgaatggttg gaaattgcgg aaccattttt ccaaaaggaa taaaggtgtt
     3481 aggtgattat tgtataatta ttgctgtttt tttatgtagt aaataaatta ggtatgatat
     3541 aaaaaaatta ttttaatcaa tatattagtg aaagcttaat atgaaaatta taaatagatt
     3601 aaaggtagca cctatataaa tagttgctac ctttttctgt ttgtaaataa atgatagata
     3661 agaaagttta ctgacaaatc actgactaaa cccaattttc ttacattgaa agccttaatt
     3721 ttacttgttt taaataaatt tacaatttct tgttataaaa tactaaattt tgttaacaat
     3781 tataattagc atttaattta tactggaggt ttatatgatt agtgtaagaa cagatttagc
     3841 tttagaagct agtgaaatgt gtgaaaagtc tcaagaagga agtagtattc ctggtgtaaa
     3901 aatagaaact aaagaattag aaaattgcat tgtaactaaa gttgaagtaa tagacaggca
     3961 aggcgcagag ataatgaata aagatatagg aaagtatata actctggaaa gtaatcttat
     4021 gaagtttgat gatgatgagt caagagaaga aatgataagt tatttaaaag aggagttagt
     4081 agatatattt ggccaagaca aaaataaaaa aaccctggta attggtcttg gaaatagaaa
     4141 tattacttct gatgcgttag gaccaaagag cgtatcaaaa acacttgtaa ctagacatct
     4201 ttttaaaaat tataataaag actatgatga tgatttcact gaggtatcag ctttaagccc
     4261 aggagttatg ggtgttactg gaatagagac tagtgaaata gtaaagtcat tggttgagaa
     4321 ggttaagcca gatagagtag tagcaataga tgcattggct tcaagaaaaa tggaaagagt
     4381 aaactctact atacagatat caacagcagg aatatcacct ggtggaggtg ttggcaatac
     4441 tagaaaatct ctaactaaag aaactttagg agtggatgtt attgcaatag gcgttccaac
     4501 ggtagttgat gcagcaactc ttacaataga tgtcttagat atggcaatag acaatttaat
     4561 agctcaatca gaagaaacag aaagttttta tgaaatgttg aaaaaactta aagaagaaga
     4621 aaaatatcat ctaataaaag attcgttaga cccatatgac aagaacttaa tagtaacacc
     4681 aaaggatata gatgatacaa tcgaaaatct atctataata ataagtgaag gcttaaatag
     4741 gtctttacat ccaggtagat tggtataaaa taaggaggaa caattatgtt taaaaaatgc
     4801 attaaagtag taaccttgac ttttatatta gcatgcattt taccaggaaa atcattagct
     4861 ttgaatcagg atgatttttt aaagttttta gtaaattcat cttatccaga agctaaagtc
     4921 gaaggaaatg atacagaaaa taaaaagaat aataaaaata aagaaacaag taaggaaaat
     4981 aaggaagaaa gtaaagaagg aaacactaaa tctaaagatg caagtaaggt tgataataaa
     5041 aaagagagtg aaaaagaata tataaaactt tatgttggta aagaaaatgt tccagatatt
     5101 gagtcaaaaa attcagacac aactgaaact aatacaacat ctagttcaga ttataaggat
     5161 gatttaagag taaccaaaga aaatccaaga attttgatat atcatactca tggatgtgag
     5221 acttattcaa attcacctga tggaaattat cattcaagag ataagaaaaa ttcagtaatg
     5281 gaagtaggaa gtgcattaac tagtgcttta gatagcaaag gatggggtgt agtccatact
     5341 actaaatacc atgactatcc atcttataat aactcttatg caagtagctt aaaaacaata
     5401 caaagtatat tacctaaata taactcagtg gatatagcga tagacttaca tagagatgct
     5461 agggatttaa ctaatccagc cactaaggaa aaagaccatt taaaatatac aactatgata
     5521 aatggagaga gagtatcaaa gttcttcttt gttgtaggag gtaaaaatac aaatagaaaa
     5581 caacttagag ctttagcaga agatataact gcttttgctg agaagaagta tcctggtcta
     5641 gtatctccaa tagtagaaaa agactatgct agattcaatc agtttgcagt taagaatcat
     5701 atgttagtag aaatagggaa taacgcaaca agtgtagaag aatctaaggc tactacaaag
     5761 tatttagccg agattttgga tgaatatttt aaacaaaaaa attagagctt taaggtataa
     5821 ttaggagcat gctaaatgag ccgattagaa aaaaatatag aaagtaaaag taaactaaga
     5881 aaatacaagt atattatgag aatcttattt ttgattacta tgattatagg tacatcagtt
     5941 tgtgtattta tagtagacag taatgcaaaa agtatgctag gaatgaaaca atatgatttt
     6001 ccaagtatta aatttgatat aacaaaattt gattttaaca taccaaatct tgatatacat
     6061 gaaatcataa caaaatttaa taagtagtaa taagtgaata aggtttattg attaaaataa
     6121 gccttatttt agttatatat ttttaatgta taaaataaaa attcattaac tgtattttat
     6181 aggatttact ttaagtttac gatattatat aatatttagt gttagactta ataatccaca
     6241 tataatgtgg ataattttgt ttagtgccaa ttagtttata ttcaaaaatt ttaaatttgg
     6301 tatataaaga gtttttagat aatgtaaaat aataactata agaataatca agaggaggga
     6361 attttaaggt ggacaataaa caaagtagaa ctagaaattt tagtataatt gcacatatag
     6421 accatggaaa gtctacctta gctgatagat taatacaaca aactgggctt gttagtgaaa
     6481 gagacatgaa aagtcaatta cttgacaata tggaccttga aagagaaaga ggaatcacta
     6541 taaagcttca aaacataaga ttaatgtata aagcgaagga tggaaatgaa tattatttaa
     6601 atcttataga tacacctgga catgtagact ttaactatga ggtatcaaga agtttagctg
     6661 catgtgaagg agcactatta gtagttgatg cagctcaagg tgttgaggca caaactttag
     6721 ccaatgttta tcttgctata gaccaagatt tagaaatatt accaataata aataaaatag
     6781 atttaccaag tgcaagacca gaggaagtaa aaaatgaaat agaagattta atagggcttg
     6841 attcaagtga agctcctctt atttcagcta aaactggttt aaacatagaa gatgttttag
     6901 aagacattgt aaaaaatgtt cctccaccta aaggagataa tgaagctcca ttaaaggcat
     6961 taatattcga ttcttactat gatgcttaca aaggtgttgt agcttatgtt agagtatttg
     7021 aaggaactgt taaaaagggt atgacaataa aaatgatgaa taccaataaa aagtttgagg
     7081 taactgaagt tggtgttatg gctccaggtc aaactgaact tagtgaatta tcagcaggtg
     7141 atgttggtta tattgctgct agtataaaag atataagaag ttgtcgtgtt ggtgatacaa
     7201 taacagattc aaataatcct acagaagaac ctttaccagg atataaaaaa gctactccaa
     7261 tggtatattg tggtatatat ccgggagaag gagaaaagta tgaaaatgtt agagatgctc
     7321 ttgaaaagct tcaagtaaat gatgctgcac ttgaatatga agctgagact tcagcagcac
     7381 taggatttgg ttttagatgt ggtttcttag gtcttttaca tatggagata atgcaagaaa
     7441 gacttgagag agaatttaac cttgatataa ttactacagc cccatctgtt atatatagag
     7501 ttacaaaaat ggatggagaa gttgtaatga tacaaaatcc tgcaaactta ccagaaccat
     7561 ctgagataaa gatgatagaa gagcctatag taaaaggaga tataatagtt cctaaagact
     7621 atgtaggtgt tgttatggaa ctttgccagg aaagacgtgg aaacatgtta aatatggaat
     7681 atatagatga gagaagagta atgcttcact atgaccttcc tctcaatgaa gttgtttacg
     7741 atttctttga tgctttaaaa tcaagaacta gaggttatgg ttctcttgat tatgaggtta
     7801 aaggttatgt agcatctaca cttgtaaaac tcgatatatt aattaataaa gagcaagttg
     7861 atgcacttag ttttattgtt cacgaaactc gtgctttccc tagaggaaag gcaatgtgcg
     7921 agaaattaaa aggggaaatt ccaagacatc agtttgctat acctatacag gctgctgttg
     7981 gtaataaggt tattgcaaga gaaacaataa gtgccctaag aaaagatgtc cttgctaaat
     8041 gttatggtgg ggatatatct cgtaagaaga aacttcttga aaagcaaaaa gaaggtaaga
     8101 agcgtatgag acaaattggt tctgttgaag ttcctcaaaa agcatttatg tctgtactta
     8161 aactagatga ataaattctt ataaatttag ataaatatat ttaaaagcta gagtgatttt
     8221 agaattattt ctaaattaat ctagtttttt tgtggtatat tttaagtaaa ctcatagaga
     8281 atataagtaa gaatatatta aagaagaata aaaaggtgat atttatgatt aatatagaaa
     8341 ttcctaaaaa agtagactat ataattaaag aattagaaac gaatggttat gaagcttatg
     8401 tagttggagg atgcgtcaga gattgtttat tagaacgaat cccaaatgat tgggatataa
     8461 caactaacgc tagaccagaa gcagtagtag aattatttaa aaaaacaatt ccaaccggta
     8521 tacaacatgg aactgtaaca gttatgattg aacatgaacc atttgaagta acaacttata
     8581 gaattgatgg aaattatagt gatggtagac atccagattc aatagaattt acaaataaca
     8641 tagttaagga tttgtcaaga agagatttta ctatcaattc aattgcatac aattcaaaga
     8701 caggattagt cgacccattt aatggctatg aagatataca aaataagtat atacgttgtg
     8761 ttggaaatcc agtagataga tttgaagaag atgcacttag aatgcttagg gcagttagat
     8821 tttcagcaca actcaatttt aaaatagctg aaggtactaa gcaaagtatt cataaaaaag
     8881 cagatttgat aaaaaatgtt tctatcgaaa gaatccaaac agagtttaat aaaatattag
     8941 tatctgattc atctaaatta aacttattaa aatcaacagg tttgttgaaa tttataattc
     9001 ctgaaatatg tgaattagaa gatgttattc aacacaatcc atatcacatc tatgatgtac
     9061 aaaaacatac tttaatagct acagaagtaa ttgaagatga actgtattta aaacttacaa
     9121 tgttgtttca tgatttaggt aaaaaagtaa ctaaaacaac tgataaaaat ggtgttgacc
     9181 atttttatac tcatagtaga gaatctgtaa agattgcaaa gaagattttg agaagactta
     9241 agtatgataa ttatactatt aataaagttt taatattaat acaatatcat gattatagaa
     9301 tagaacctaa aaggaagata attaaaaagt tattaaataa acttgaagat gttgaattat
     9361 ttgaagattt aataaaagtt aattgggctg atacacttgc caaaaatcca aagtatgcaa
     9421 aacaaaagat attaaatctt attgagtgtg aaaaagaatt taaacatata ataaatcaaa
     9481 aggagtgctt taatcttaaa gatttagcta ttaatggaaa agatttgata tctataggta
     9541 taaaaccagg taaagatata ggacatattt taaataagat gcttgaaata gtcataaata
     9601 atccagaatt aaatgaaaaa gaaatactaa aagaaaaagc attaaatata tatacatttt
     9661 agattcaaat ataaaaaatt taattacttt aaaaaatata gaagaaaata cttatttaga
     9721 taaaataaat aatttttaga gaatatttta gagattacga ttgtatgatt tttaaacgca
     9781 tagcatttac tctaggacta tagactaata taaaaaaaaa ctttgggaat actaaaacca
     9841 taaatttata aaattaatat tttatatact gtaaaacagt gaggagggac agtatgcata
     9901 ctcaactaca aaagactatt ggtctttcag cagctctttc tactgttgta ggtatggtta
     9961 taggttcggg agtttttttt aagccacaag caatatacac tacaactaat ggagctccag
    10021 gtctaggtat tatagcgtgg cttttaggtg gttttataac aataactgct gggcttacag
    10081 ctacagaaat ttcagctgct ataccaaaaa ctggtggtat gatgatttat atcgaagaaa
    10141 tatatggaga aaagttaggc tttttaactg ggtggatgca aactgtatta tttttcccag
    10201 gtacatcagc agcattagga gtaatatttg ctcaacaagc atctgaactt ttaggaatga
    10261 gcccaaataa tatggcaaat gtacttccta tagcaattgg agttatacta tttttagcat
    10321 tattaaatat aataggttca tctttaggag gaaaagttca gacagtagcc actattggaa
    10381 agatgatacc tttaatttta ataatagtct ttggatttat taaaggtcaa agtagtgaag
    10441 tattaaatcc atttgtagga gatggtgtaa atgcatcaaa tgcacttgga caagtattaa
    10501 tagctacttt gtttgcgtat gatggttgga taaatgttgg ggctatatca ggagaaatga
    10561 agtctccaga gaaagattta ccaagagcaa tagttggtgg tttatcatta gttatggcag
    10621 tttatattat aataaatata gcttatcttt gggtagtacc agcaagtgaa ttagcaacag
    10681 ttacttctcc agctacatta gtagcaacaa gattgtttgg aaatataggt ggaaaagtaa
    10741 taactgtggg tatattaata tcagtatttg ggacattaaa tgggtattta ttaacgggtt
    10801 caaggatacc ttatacatta gctgaaatgg gaactttacc agcatctaaa actcttttaa
    10861 aagtaaattc tggaggttct cctgtaaatt caatactact tataactgtg ttagcctgtg
    10921 tatatgcatt atctggtcaa tttaatcttt tgacagactt aactatattt tcaatatggg
    10981 tattttatgt attgactttt ataggtgtta tgaggcttag aagacaaagg ccagatttac
    11041 acagaccata taaagtccct ttatatccaa taataccaat tatagctata ctaggtggac
    11101 tgtttgtaat aataaatcaa atattgactt caacaatcat atctttaggc ggaatattta
    11161 taacactatt aggtttacca gtttattatt atatgaaaaa aagataacta cttaatttaa
    11221 gttagataaa tttgggtaaa ggtgaaaatt aacttttatc caaatttttt atactgttat
    11281 acaatataag aattatatat tacttgatgt gttattttgt ttgatttata atttagtaat
    11341 tgatgcttta agataattta agaaatgagg aataaaaaat gctaggactt tatgtacata
    11401 ttccattttg tgtgaaaaaa tgtaagtatt gtgattttaa ttcttataaa atggacatag
    11461 attcaaaaaa aagatatata gaagacttaa aaatagaaat gaaattatat agtaataaat
    11521 tatataaaga taataaatat aaaaataaag aatgctgtag tctaaataaa aatgacaaaa
    11581 taacgagtat atttgtaggg ggaggtacac ctagtatact aacatctgat gaaataagag
    11641 aagtatttat aagtataaaa gaaatgtttg atatagatga aaatgcagaa ataactatag
    11701 agtgtaatcc tgggacatta acattagaaa aattaaaaac tatgaaagag attggcataa
    11761 atagacttag tatagggcta caagccattc aagaaaagca tttgaatttt ataggaagaa
    11821 tacatactta tgaagaattt gagaaaaact ataaagatgc attaagtgta ggttttaaaa
    11881 atattaatgt agatttaatg tacagtcttc caaatcaaac tctatgtgac tggaaggaaa
    11941 ctcttgaaaa agttgtacac ttaaatccaa cacatatatc agcgtattcc cttatactag
    12001 aagaaggaac tgaactatat aatatgtatg aaagtaataa atttgagctt atagatgaaa
    12061 atgtagatat agaaatgtat gagtatacaa taaactactt aaagtcaaaa ggatataatc
    12121 aatatgaaat atcaaattac tcaaaagaag gctataattg tgagcataat attttatatt
    12181 gggaatgtga acattatata gggattggag caggagcttc tggctatatt aatgagaaca
    12241 gatacaataa tgtagaatct ttagaagatt atcatttaag tttggtaaaa agagaaaaac
    12301 caatacaaga aaatgaaatt ctttctgaga aagatatgat agaagaaaag atatttatgg
    12361 ggcttagaat gaataaaggc ataaaatttg aagattttaa aaaaaaattt ggaatagatt
    12421 ttagagagaa gtataataag caaatagaaa tgctattagc tagaaagctt ataaatcaga
    12481 gctttgaagg tatacaatta actcaaaaag gaagagaaat atctaatagt gtatttatag
    12541 aatttatgga ataattttta aggtgttgac aaaataaatt tataatggta tataatatat
    12601 ataatctttt tagcactcga acttagtgag tgctaacagc tgggaggtat aataatatgg
    12661 aattaaatga aagaaaatta aatatcctca aagctatagt taaagattat atagaaacag
    12721 ctgaagctat aggttctaga acaatatcta aaagacatga tttaggtgtt agtgctgcta
    12781 ccataagaaa tgaaatggct gaccttgaag aattagggta tttaattcag cctcatactt
    12841 ctgcgggtag agttccatct gaaaaaggct ataagttata tgtaaactct cttatgagta
    12901 aaagcgaatt ggatgataat gataagattc ttattgaaca atgtatgaat cataatataa
    12961 atcatataaa agaattgatt catgaaactt ctaagctatt atcgcaacta acaaactata
    13021 caactgtagc agttactaag agtttaataa atcaaagtgt tataaagcac atacagttgg
    13081 ttgctatgaa tgacaataat attttactta tagttgtaac agataaaggg gacttaaaaa
    13141 aagctaacct tacaactaat gtatatttag atcaatctaa gttaaatttg atttctgata
    13201 atcttacaag gaagctctta ggtaaaagta taacagattt ggatgataat ctaattgctt
    13261 ttattaaata cgaaataagt gagtattctg ggcttataga tgagctttta aatgctttga
    13321 actcaaatat gaaagaagaa gatttttctc tatcattaaa tggagctact aacatattta
    13381 gttatccaga atttaatgat gtattaaaag caaagtcatt tttaaatatg ttggaaaaaa
    13441 aggaaacaat agctgacata attaaatcaa agggaataca aaaagacaat ctaaatataa
    13501 taattggtag tgataatgat tgtgaactgg cacaggattg tagtatagtt acagcaactt
    13561 ataatgtaga tagagacttg gttgggcgaa taagctttat aggccctacc agaatggatt
    13621 atgctagaat ttactctata ataaattata tgagtttatt aattaataga aagtaaagaa
    13681 attttaaatt gcgaggtgta aagtcttgga taaaaaaaat gagcaacagg aagtaagaga
    13741 agaaaatgat acttctatta accaagaatc ggaaactcaa gtagagttag aagaggaagt
    13801 tgttaatgaa gaatgtgaaa catcaagtga aaaaactgat gaaaaagaag ttgatgatga
    13861 aaatgtaaca gatataaatt ctaagttagc agaaaaaaaa ttgcaggatg agttggatga
    13921 attaaatgat aaatatcaaa gacttcaagc cgaatatgca aactacagaa gaagaactca
    13981 acaagagaaa gagacaatag gcgtatttgc aaatgaaaaa ataataactg agttgatacc
    14041 agttatagat agtatggaaa gagccttaga tgcatgtgaa aataaggaag atactatgta
    14101 taagggtata agccttgtac ataaacaatt aatagacact ttggtaaaat ttggagtaga
    14161 agaaatagaa gcagaatcta aggaatttga ccctaatcta catctagcag ttatgcaaga
    14221 aagtatagat ggagttgaag caaatcaaat agtaatggta cttcaaaaag gatataagct
    14281 aggaactaaa gttgtaagac cttcaatggt taaagtttct tgctaagtat aaattaaagg
    14341 ttacattatt taacctaata taaaatatta aaataagtaa aacttaatta tataattact
    14401 cgaggaggat tttagtcatg ggaaaaataa taggaataga tttaggaaca acaaattcat
    14461 gtgttgcagt gttagaaggt ggagaagcac aaataatagc aaatagtgaa ggtatgagaa
    14521 ctactccatc agtagtagca tttacaaaag atggtgaaag aatagttgga gaacctgcaa
    14581 aaagacaagc agttacaaat gcagataaaa caataacttc tataaaaact catatgggaa
    14641 ctgattataa agtaaatata gatggaaaat catatactcc acaagaaata tcagcaataa
    14701 tattacaaaa attaaaatca gatgcagaaa gttacttggg acaaacagta acagaagcag
    14761 ttataacagt accagcttac tttacagatg ctcaaagaca agcaactaaa gatgctggta
    14821 gaatagcagg gcttgatgtt aagagaataa taaatgagcc aacagcagca gcacttgctt
    14881 atggtatgga taaattagac caagaaaaga aaatattagt atttgactta ggtggaggaa
    14941 cttttgacgt atctatactt gaaataggag atggaacttt tgaagtttta gctacagctg
    15001 gtaacaatag attaggtgga gatgactttg accaaatagt aatagattat ttagcagagg
    15061 aatttaaaaa agctgaaggt gtagatttaa gaaatgataa aatggctctt caaagattaa
    15121 aagaagcagc agaaaaggct aagaaagaat tatcatcaac aatgagctca aacataaact
    15181 tacctttcat aactgctaca gcagaaggac caaaacactt aaatatagat ttatcaagag
    15241 ctaaatttga agaattaact agaggtttag ttgaaaaaac tatggaacca actaagacag
    15301 ctttacaaga tgcaggactt tctacaggtg atatagatga tgtattatta gtaggtggtt
    15361 ctacaagaat accagcagta caagaagctg ttaagaagtt tataggaaaa gagcctcaca
    15421 aaggtataaa cccagatgag tgtgttgcag caggtgcttc tatacaagct ggagttttag
    15481 caggggatgt taaagactta ttattactag atgttactcc attatctctt ggtatagaaa
    15541 ctatgggtaa tgtaatgact aagataatag aaagaaatac tacaatacca actaagaagt
    15601 ctcaaatatt ctcaactgct gctgataatc aaacagctgt tgatatacac gttcttcaag
    15661 gtgaaagaag tatggcttat gacaatacaa ctttaggaag attccaatta acagatatac
    15721 caccagcaca aagaggaata cctcaaatag aagttacttt tgatatagat gcaaatggta
    15781 tagttaatgt ttcagctaaa gatttaggta caggaaaaga gcaaaaaata actataacat
    15841 caaacactaa tttatcagaa gcagaaatag agcaaaagat aaaagaagca gaaatgaatg
    15901 cagaagctga caaacaaaag aaagaaaaaa tagaagcatt caatcaagct gaatctacta
    15961 tatatcaaac tgaaaaaact ttaaatgaac ttggagataa aataagttca ggtgaaaaag
    16021 aggatataga aaaagctata gcagacttaa aagctgtaaa agataatcaa gatgctacag
    16081 cagaagaact taagaaagct acagatgaag taatgactaa gttccaaaaa gtatctcaag
    16141 aaatgtacca aaaagcagct caagaacaac aagcagcaca aggtgcagaa caagcacaag
    16201 acaatggacc taaagatgac aatgtagtag atgctgactt taaagaagtg gatgaagata
    16261 aataagctat ataatatgta acactaaatg ataattggaa catatatgat ttgtgaaaat
    16321 catatagatg ttaaagatat atggacaata cacatcataa agaatataat atatcataga
    16381 ttatataact taaagtagaa tataagaatt aatgatagct tgtagtttat tattctacaa
    16441 gctatcgttt tgtaaaatga aggtggtgtc aatttttgag tacaaaaagg gattattatg
    16501 aagtcttagg tatcagtaaa ggtgcagaag ctcaagagat aaaaaaagct tatagaaaat
    16561 tagcaatgaa gtaccatcca gatagaaatc ctggagataa agaggcggaa gaaaaattta
    16621 aagagataaa tgaagcttat gaagttctat ctgatgatac aaagagaaaa acttatgacc
    16681 agtttggaca tgatgggcta aatggtcaag gcggttttgg tggtcaagga ggatttggcg
    16741 gtcaaggctt tggtggtttt gaagatatgt tcggagatat atttggtgac atgtttggag
    16801 gaagctttgg aggtggaaga acaagaagaa gagggcctca acgtggtgca gatataagac
    16861 agagtgttac tatatctttt gaagaagctg catttggtaa aaagatgtct ataaaagtaa
    16921 atagaagtga agagtgtgaa gaatgtaatg gaactggagc aaaaccagga acatcaaaga
    16981 aaacttgttc aacttgtaat ggaactggtc aagttagaac tgttcaaaga actccatttg
    17041 gtaatatagc aagttcaaga ccatgtagtg cttgtaatgg aactggagaa gtgattgaat
    17101 ctccatgtag taaatgtcat ggaactggaa atacaagaaa agttaagact atagaagtag
    17161 atatacctgc tggtatagat gatggtcaaa tgataaaact ttctggtcaa ggtgaagtag
    17221 gagaaaaagg tgctccaaga ggagacttat atattgtagt taatgttaag tcacatccac
    17281 tatttactag agatggaaat gatatttatt ttgaaatgcc aattacattt gttcaagcaa
    17341 ctttaggtga tgagatagaa gtacctactt tggatggaaa agttaaatat agtgtaccag
    17401 aggggacaca aactggaact gtatttagac ttaaagagaa aggaatccct agaattagag
    17461 gtaattcaag aggagaccaa tatgttaagg ttgtagttga aattcctaaa aaattaaacg
    17521 ataagcaaaa agaactattg agagaatttg cgaaggagtg tgggtcgaac gtccacgaaa
    17581 agaagaaaac cttcggacaa aagatagaag atatgtttaa gaaaaagtag ttaaagtagc
    17641 caatatataa tcatattttt gaaatcttag ttatttataa gattgtcaaa ggagatatta
    17701 tgatttttat attggctatt tttgtataaa aaataaatat gcataaaata aaactgaata
    17761 taaataattt tctgaaaaat acttgtccta aaatatataa tatgctataa tataatcaaa
    17821 taaaagaaag gggtgattcc agtgggaacg gatgtggcac tgccaaatga cgaagaaaac
    17881 tttatggtaa tctacaaaat aaaattagat acttttagtg ctagattaag tactggaaaa
    17941 actcatgagc tgggataatt attgtaatta cctagactca aacttttagt agtaaaatag
    18001 taatattata tagacaaggt atctaatttt taaatctttt gtatgaatta aaatttttat
    18061 aaagggtata tctgattaga atgtaattag ggaaacctag ttgtgaatta cttttagtat
    18121 atacatgtgt aaagaaatat gtatatggta ttggttattg tgctagatgt gtatgtaaag
    18181 gaatatgtgt ctagttgggt atatgtaaag aaatatactt ataatctatg aaaaattttt
    18241 agtataaatt gctaaaacat tgagattgtt ttttatagaa agttagttta taccaactaa
    18301 aaatgtgctt aagatttgtt tgatgtttaa ttataaataa ggttgtagat aaaaaaataa
    18361 gtagagacaa aggtttgaaa tacttgattt aaatatagtt tgttttgaac acttgccaaa
    18421 ttaaaataat taataaaata gcatcttctc atagtaaaat atactgtgag aggatgtttt
    18481 tgctttgttc ataaactttt atgaaaaatt tgcatagtgt cacattttaa tatactatat
    18541 atataataaa atatagataa aattaaatga ttcaaaagag aaaggtgaaa agagatgtat
    18601 tatataggtg tagacatagg aggaacaggt atacaagcag gagtagttga taactatggg
    18661 aagataattt ttaggtcaga gtgtaaaaca gtaattgaaa aaggatttga aggaatactt
    18721 aatgacataa aaataatgat ttataagttg ttggaagata ataaacttac tatgagtgac
    18781 ataaaatcta ttggatttgg tgttccaggt tttataaata aagaaggatt agtaacttgt
    18841 gtaaatttaa aatggaataa aaaagctttt aataaagagt taaaaagaag atttccagat
    18901 gttgaaattc atggagaaaa tgatgcaaca gtggcagcac ttggagaagc aaagtttgga
    18961 agtatgaaag gtgctaatgt tggagtatta tatactttag gaactggtgt aggtggagga
    19021 atagtaatta atcaaaaagt cttttctgga gcacatggat tgggttcaga aataggtcat
    19081 caaattatag gagaaaacta ttttaattgt aattgtggaa ataatggttg tgtagaaact
    19141 ttttgttcag caactgcaat tataaagtat tctcaaaagc tgatagaaga aggagaaaag
    19201 agtaggattt tagatttggc agagggaaat ttagaaaatg taaatgcaaa gatggtgttt
    19261 gatgcatata gagaaaatga tttagtggca attaaagtaa taaatagatt taaagaatat
    19321 ttagcaaaaa cttttgcaaa cacaataaat tctttagacc cagaaattat atctatagga
    19381 ggaggtattt ctaaatctag cgatataata cttgatggaa tagaagattt ggtaagaaaa
    19441 tttgtattat acaaaacaga agatatagct acaattacat gtgcaacttt aggctcagac
    19501 gcaggaatta taggagcagc ttttttataa tgtaaatgaa ctgcttattg gaggcgtgat
    19561 gatatatggg taaaacaaat gctagaagaa cgcttttttt gatagcaata ggttcaggaa
    19621 ctatgctgaa tcctcttaat tcttctatga tttcacttgc tttacatagt atacaaaatg
    19681 attttcatat tagttttaca actgtatctt ggattgtttc agcattttac ctagctagtg
    19741 ctattgcaca accagtttca ggtaagatag gagatttaat aggtagaaag gtcttatttt
    19801 tgagcggttt gatgcttgta attttatcag catccatagt accacttgtt caatcgttct
    19861 ttatattgat ttttataaga gtaattcagg cgattggaac aagtacacta tatccatctg
    19921 gggttgctct tatacaaaat aatataaagg aaagacaatc ttctgcacta gcagttttaa
    19981 ccatttttgc ttcaactaca gcaggattag gtccgacact tggaggatta ttgttggatt
    20041 taggaggatg gcatgctata tttcttgtaa atatacctgt tgttttggtt tctatatgct
    20101 tagggtattt tttattccca aaagaagtaa aagaaaagaa atctataaaa gaaactctga
    20161 aaaatgtttt gtcaagatta gatattattg gcatacttct attttcaatt gcaatgattt
    20221 ttatattatt atttttgtta tctatgaagg agtctttcaa cttagaacaa cttgtatttg
    20281 gtataatact gatgtgtctt tttatttggc atgaattgag aacaaaatta ccatttatag
    20341 atattaagct ttttgtgagt aaccctaaat tgtcaaaagt ttacttgcaa tttattatat
    20401 taaatttctt taattatatt ttgttttttg gtcttcctag ttattttcaa gatgcattac
    20461 actatagtgc aaaatcgact ggattattta tgctatttat gtctggtata ggcatattta
    20521 tatctccatt aactggaaga tggatagata aatctggaac tagatttcct cttgttacat
    20581 catcaatctt tatgtttatt agtgcagtac ttctttcagt agtttttatt catataccag
    20641 taataggaaa aggtatcata ttatcattag caggtattag ttatggagtt ggtaatgtag
    20701 ccctgcaatc ctctatgttt gaagaaagtc ctcgtgacag tatagggaca gcttctggtc
    20761 tgtttcaaac tagtcgttat ttaggttcta ttttagcaac tgttttactt ggaatggtat
    20821 ttgaagtaaa tataacatca gaacaatttc agatattagg ttatgtgatg gtggtcttag
    20881 gtacaataag ttttttatta aattttatat taaagaaaga attaagaaaa gtagaatagt
    20941 ggatttatgg atttaataag tctaataact gataagttta aaaatattgt ttgtaaaata
    21001 taactagttt gatatttatt aattctaagt tttgaagcat agagtactcg ataagtatac
    21061 aatattttga attaaaacta agcagaaatt tgaactagtc tttcaaatct ctgcttaatt
    21121 tagtggttaa atttttttat agagaaaata agatttgaca ctcagataaa aaatcattgc
    21181 attttcaaaa ttctttaaat caaacccaag tatctcatta atcttttcca atctatatac
    21241 tagcgtgttt ctatgtaaaa acatactagt tgatgtctta gaaatattta aatc
//
LOCUS       DAEMAE010000048        21172 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEMAE010000048 DAEMAE010000000
VERSION     DAEMAE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.049"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..246)
                     /locus_tag="KPH93_001439"
     CDS             complement(<1..246)
                     /locus_tag="KPH93_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688268.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQ"
     gene            complement(500..2200)
                     /locus_tag="KPH93_001440"
     CDS             complement(500..2200)
                     /locus_tag="KPH93_001440"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG1688269.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKDIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(2559..2972)
                     /locus_tag="KPH93_001441"
     CDS             complement(2559..2972)
                     /locus_tag="KPH93_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG1688270.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLRIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEKGIKVKSEKDL"
     gene            complement(3047..5425)
                     /locus_tag="KPH93_001442"
     CDS             complement(3047..5425)
                     /locus_tag="KPH93_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG1688271.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPVDYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIERLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            5743..7194
                     /locus_tag="KPH93_001443"
     CDS             5743..7194
                     /locus_tag="KPH93_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG1688272.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINNILTAFKHEFTTSDPGMTYSIAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(7398..9911)
                     /locus_tag="KPH93_001444"
     CDS             complement(7398..9911)
                     /locus_tag="KPH93_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1688273.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFDINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYYGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSKTRLKELDEYCKINSMSPELVYCKTNDEQLEALKNGKADVLLNVSMNQIEGVRTV
                     AKFSPRPFYFITPKGSTDLTQNLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKNHEIDIVAGMIYDYEYSREHNVAMSRPFISTQYTMMINNKLNENSLDGK
                     RIALTKGINHDGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPERGIETYYGLDKNDKEGENGNTTNEIDKIRREYNKKFFNVIKSQE
                     NGTTEMYSIMFDDTFHWIRITSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTKKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKALDESSANDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHFIKLPNGESLS
                     ISMGVAISRRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(10134..10955)
                     /locus_tag="KPH93_001445"
     CDS             complement(10134..10955)
                     /locus_tag="KPH93_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1688274.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIEGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDISLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(11408..11884)
                     /locus_tag="KPH93_001446"
     CDS             complement(11408..11884)
                     /locus_tag="KPH93_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG1688275.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDMLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFFD"
     gene            complement(11918..12496)
                     /locus_tag="KPH93_001447"
     CDS             complement(11918..12496)
                     /locus_tag="KPH93_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG1688276.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRDLAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(12600..15182)
                     /locus_tag="KPH93_001448"
     CDS             complement(12600..15182)
                     /locus_tag="KPH93_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG1688277.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     KNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDNKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKNGEITTIAHKGDLIELDFVGEAKRNQIIYKTSDSEL
                     MGRVQATFQQDKEFIKTNISAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLSYDVDLIYYEDINTLSK
                     ALQMVSNISTDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEVEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESNYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKNATDLYKKLDEIGTVAGHYY
                     KGVE"
     gene            complement(15713..16396)
                     /locus_tag="KPH93_001449"
     CDS             complement(15713..16396)
                     /locus_tag="KPH93_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG1688278.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFVPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVAFGIMFI
                     NKDDSFNEN"
     gene            complement(16737..17327)
                     /gene="zapA"
                     /locus_tag="KPH93_001450"
     CDS             complement(16737..17327)
                     /gene="zapA"
                     /locus_tag="KPH93_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG1688279.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(17352..19745)
                     /locus_tag="KPH93_001451"
     CDS             complement(17352..19745)
                     /locus_tag="KPH93_001451"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG1688280.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAINGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAVQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVADDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(19762..20781)
                     /gene="pheS"
                     /locus_tag="KPH93_001452"
     CDS             complement(19762..20781)
                     /gene="pheS"
                     /locus_tag="KPH93_001452"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG1688281.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITTILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(20831..21064)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         6494 a         3896 c         2320 g         8462 t
ORIGIN      
        1 ttgaatatta gtatttgata aaattagtgg taataaaata tttaaaaaat tgatatttcc
       61 tattttttct ccataaaatc tatagtaaat aagttcatct ctcagtctat tttcgtctat
      121 atacacacat ctttgcgttt tatcatctat taaggatttt gtatttatta tagttaaatc
      181 tttaagagcc ctacttgcac tactaaaaga agatgtgtag acttttttta gtaattcgtc
      241 attcataaat atatcctttc tgaaaaattg aatgtaaata aattgttatt aaaaaaaaag
      301 agacagcccg tttaagaagc tgtcatatat agggggatag tagtgtacaa aaatcattgt
      361 aatctactat ctatatatta ttatacgata ctactttttt atatgtaaag catcagtttt
      421 cgacatccct atattttaca gttatacaaa ttgcgtacta agttcaattt tattcttttt
      481 atttcaacat tttatcatgc tacattcttt ccggagcgtg cattcccaac aatttcagag
      541 aattttctaa agtttgtttt gttgctaaaa ctaatgcaag tcttgctttt cttatttcca
      601 aattttctac taaaattgga ttgtcatgat aaaatttatt aaacgcttga gctaaatcta
      661 gcataaatct agtcactata tgtggctcat ttttctttag agcaagtaaa atattcttat
      721 tgaatgattc tataactcta agaacttctg cactatcttc atcagctaat agactataat
      781 ctatatcaga agtcacttct acttctgctt ttcttaaaac tgcacagcat ctagcatgtg
      841 tatactgaac atatggtcca gtctcaccat caaaactaag tgttctttcc caagagaaag
      901 tatagtcttt aattctacta ttagataact cttggaatac tactgcccca acacctactt
      961 gcttagatat ttcatccaca tttttagcat ttgggttttt agctagtata atatccttag
     1021 tcttatctat agcttgattt aaagcatctt ctaaaaatac gactcttcct tttctagtcg
     1081 acatagtacc ttcttcaagt gcaaccatac caaatccaac atgaattaaa tcttttgccc
     1141 aatcatatcc cataagctca attaccttaa accattgttg gaaatgtaag ttttgttgag
     1201 aaccaactac atatatacac ttgtcaaaat cataagtttc ttttctatat attgcagcag
     1261 ctaaatctct tgtcatatat aatgttgaac catcattctt agtaataagt gctggtggca
     1321 ttttatactc ttctaaatca acaattcttg ctcctttaga ctcttttaaa agatttttct
     1381 cttctaatag ttcaatgact ctgttcattt tatcagaata aaaactctct cctgctaaag
     1441 aatcaaattc tatatctaaa agcttataca ctctgttaaa ctcttttaaa ctctcatttc
     1501 taaaccattg ccataattct tgtgcttctt cgtcaccatt ttctaattta ttaaaccatg
     1561 ctcttgcttc atcttccatt tctgggtgtt gttcagcttc atcatggaat cttacatata
     1621 attttagtaa ttctggaatt ggattagact ctactacttc tttttctccc cactttttaa
     1681 aggccactat taacttacca aactgagttc catagtcacc taaatgattt attcttatag
     1741 tcttgtatcc ttgggaatca tatatcttat ataaagcatt acctataact gtagttctta
     1801 tgtgccctat atggaaaggt tttgctatat ttggagaaga atattcaact attacagttt
     1861 tatcttttcc aaaatcacta tgtccgtaat tttccttttc atctaaaact tttttaatta
     1921 cagtttctgc taattgagat ttatttacaa agaaatttac atatccgcct aattgtatta
     1981 cttttgttat ctctccactt ggttctatac tttccgccaa ttcacttgct atcatgtttg
     2041 gagcttttct aaacactttg gctaatttaa agcatggaaa agcatagtcc cccatatcct
     2101 tatttggtgg aacttctata agtgcctcta tctcttcttt cgataaatct tctatttttt
     2161 ctttaaggca atttgatatt gccaccttga aatcttgcat tctgttgctc ctctctacat
     2221 tgttcctggt ttatattgtg cttatctctt tatgttaaat caaatttcgt gttttttcaa
     2281 gtatttcatt acagtgtaaa acactttaat aattatgttt tctgtatata ttaaaaatac
     2341 tataattgca gtttttaatc actctattat tgatttttta taagactttt ttctatttta
     2401 ttgtttctta tactatccag ttgactttat atactttttt tactctttta tcaattttca
     2461 tatatcaatg ttaactttat atactttttt tactctttta tcaattttta tacatcaacg
     2521 ttaactttac atactttttt tactcttcta tcaatttttt ataagtcttt ttcactctta
     2581 acttttattc ctttttcttc taatagcctt gctgttatac ctttccctgg tattttttta
     2641 cctgaaaaac taccatcata tattagatta catccacaag aggggctacc ctcttttagt
     2701 attgcttcgc tacatacata taaatttgct attcttagag tttcattagc ccctcttaca
     2761 aagttttctg ttacatcctt attatcttta gtaattactt ttccatcaac tatctcagca
     2821 ggtggtcttg gtgtagatag accacctaac tgctctgggc atacaagtgt aaattctttg
     2881 ccttttaaat attccttgac tttttcattt tcattattat caccactata tttacaattt
     2941 attcctatta aacatgcaga taccaatatc ataaaaatgt gctcctatac aaattcttaa
     3001 tatattctca tacaaattac taatgtattc ttatacaaat tacaaattat tttaatttaa
     3061 caattgttac tcctgctcca ccttctccat attgaccacc tctttgtgat tttacatgtc
     3121 tatttcttct taaaatgtct tgtagccctg cttttaatac tcctgtacct attccatgta
     3181 ttactgttac agactctaaa cctgccacat atgcatcatc aaggtacttt tctacttcca
     3241 ttatagcttc ttctaggttt aatcctctaa ggtcaacttc attttttaca ctaccagatt
     3301 ttgaccttat aatgtttctc gttgatttag ttacatttgt tgaaacatct ttgcgtgttt
     3361 tttgtaaaga tttaaaaggt agggtcatct tcattatacc aatttgcact actgcttctt
     3421 ttctcttttt atctactgaa actacactac cattttgatt taaagttatt accttaactt
     3481 cttcacctgg ctttaagtct tttatctcct tatttgatac ctttggaact atcatacttt
     3541 ttactgttgg ctgaagactt cccatagaac tggtcaattc ttttctaagt tcttctatct
     3601 tacgattctt ttcttttgaa gctctttctt gttcaagact tctaagttct tttattataa
     3661 tatctacttc ttctttagct tgtcttataa ttgaaaatgc ttctgattta gctttttcaa
     3721 tcatcttatc tctttgactg accaattttt caagtttttc gtcatactct actttcagtc
     3781 tttcaatctc ttcttttaat ctttcagctt cttctctatc ttctactgct ttgattctat
     3841 tcttttcaac attctgtaac acatcttcca aagctatgtt ttctgtattt atatattctt
     3901 tagcacgtga aatcacataa tcacttaatc caagttttct agaaatctca aacgcatttg
     3961 attttccggg aacacctata agtaacttat atgttgggct taaagtttct atatcaaatt
     4021 caactgctgc gttttctaca cctgatttag taagagcata gtttttaagc tcactatagt
     4081 gagtggtagc tatacattta gctcctacag aatttatatc ttctaatact gctattgcaa
     4141 gggcagctcc ttcaacaggg tctgttcctg ctcctaactc gtcaaatata actaaagagt
     4201 ctgctgttac gttttgtaat atagatacta tattcgtcat atgagatgaa aatgttgata
     4261 gactctgttc aatactctgt tcatctccta tatctgcaaa tacattatca tatacacaca
     4321 tactgcttcc ataatcaaca ggtatatgaa gtccactttg tgtcataagt gcaaatagac
     4381 ctacagtttt tattgtaaca gtcttaccac ctgtgtttgg acctgttatc accaatgtgt
     4441 ggaagtctct tcctaaataa attgtatttg ctactacctt ctttttatcc aacagtggat
     4501 gtcttccatt ttttatgttt aaatatttat cctcatttaa agttggttct attcctctca
     4561 tctgtataga aagttttccc tttgaaaatg caaagtccaa tcttccaagt atctcttgat
     4621 tagagattaa atcttcactc acttctccaa ccatagcaga aagctctgat aaaattcttt
     4681 ctatttcctc ttgctctcct agtttcaact gtctcaattc attgttcatc tctactatag
     4741 tcattggctc tataaaaagt gtcgcaccag aagatgactg gtcatgtact ataccagcta
     4801 cttgcgacct atattcagct tttacaggta ctacaaatct atcacctctt agagaaatta
     4861 ttgcatcttg taagtacttt tgataagtag ttgaacttat tattgaattt aattttgacc
     4921 taatggattg atttttttgg gctattcttc ttctgatatc tctaagtgta cttgaagcat
     4981 tatcagatat ttctacttca ctaattatag cattgtatat ctggtcttct atgtccttgt
     5041 atacatatag agaatttgat agagattgta ttattggata attaaaatct tcttcatcac
     5101 tactagataa gctatttctt aatcttctag ccactcttaa ggtatctgct atcataatca
     5161 gacttcctgg gtcaagagaa gcacctatat aagctctctt taccttatct tctatatcat
     5221 gaataccttc caaccctact gaacctctct ttataataat tgattgagct tcactagttt
     5281 cttctagcat agatttcact tctacaaaat cagtatttgg tactaaattt tctatatatt
     5341 ttagacctaa agaagatgag gctttcttct ttaataaatc tattatttta ttatattcta
     5401 gaactcttaa cgatttctca ttcattttct atattcacca tcctcgatat gttaactttt
     5461 ttatttaatt ttaaaaatgt atgttttcaa gcatttccaa taaaaactgc aagttttatt
     5521 tctttttcat ttctaaaatt attatatttt ttctctttta gatatatttt acacatattt
     5581 tgtattatac tatgtatgta gctatctata tattatacat aaacatgctt tatttgtaat
     5641 ataatctgtt aaaaaatctt ctttaatatt tttaaaggag tttattttag cttataaaaa
     5701 aaatagctac ttatatagat aaattaagga ggaacatgag tcatgggaaa cgtattagaa
     5761 ggattaaaac cagaaagtgt ttttaaaaac tttgagaaaa tatctcaaat tcctagaggt
     5821 tctggaaatg aaaaaggcat aagtgatttt ttacttagct ttggtaaaaa tttaggtctt
     5881 gaaactattc aggatgaaag cttaaacata ataataagaa agcctgctac aaaaggatat
     5941 gaaaattgtc caggtgtagt tcttcaagga catatggata tggtttgcga aaaagaaaag
     6001 aatgttgaac atgattttct taaagaccct ataaaattaa gaattgatgg tgacatgata
     6061 tatgctactg gaactactct aggagctgac aatggtatag ctgttgctat gggtatggct
     6121 atattagaag ataatacttt agagcatcct gcactagaag ttttagttac tgttaatgaa
     6181 gaagatggta tgaatggtgc agatgcacta gaccctagct taattaaagg tcaatatatc
     6241 ctaaatatgg actccgagga ggaaggatac ttattagtta gttgtgctgg tggtaaaact
     6301 tgtgttgtta gtttacctgt tgaatacaaa aaagttgaag gtgacaaaca aggattatta
     6361 gttgaggtta ctggactatt aggtggacac tctggtatgg aaattgtttt acaaagagca
     6421 aatgctaaca aagcaatagc tagagtatta agtgtattga atgttgatta tgaattagct
     6481 tctgttgatg gtggtactaa gcacaatgct atacctcgtg aagctaagtg tgtaatagct
     6541 gttaataaag ctgatgttga atctgctaaa aaacaaataa ataacatttt aactgcattt
     6601 aaacatgagt ttactacttc tgaccctggc atgacttact caatagctga aacttctgta
     6661 gacaaagttc ttactaaaga ttgtaaagaa aaagttgttc aaatgtcttg cttaactcct
     6721 catggagttc aatcagtaag tcttgatata gaaggattag ttgaaagttc tactaacttc
     6781 gctataattg aaactaaaga atctactatt gaatttttaa cttctgttag aagttctgtt
     6841 atgtctatta gagatgaaat cgctgataga ataagacttt tggctcaagc tttaggtgct
     6901 aattacgacc ttatagctca atacccagct tgggaattta aaaaaggctc aaaattagaa
     6961 aaaatatgca gtgaaactta tgagaaatta actggtaaag ttcctactgt tatggctcta
     7021 catgctggtc ttgagtgtgg attattactt gataaacttc ctcatgctga agctatctct
     7081 ataggaccag atatgtttga tgttcatact ccaaatgaac atgttagtat cccttctgta
     7141 gctaatgttt gggattatgt tattgaaata ttaaaatcta tgaaccaata ttaaatttaa
     7201 gttttgattc taaatagatt ttaattttaa gtaatctata taaaattaac tctgtaaatt
     7261 aatactacta ttagtataat atttactttc aatattaact tatagaaata aagcctaatg
     7321 ataggtaata atatgtatca ttaggcttta ttgtaattta aatattatta aactttattc
     7381 tgttttaaaa ttaattctta tactatttta aatctatttc ttccttgtct cttagaatcg
     7441 taaagagcat tatctgctat ttgatacagt tcatcatact cttgaccacg ccttgatata
     7501 gctaccccca tactaataga taaactttct ccatttggca acttaatgaa atgaatatga
     7561 ttgtatacat cattacactt attgataagc tcttccttat ttattatatc attgatataa
     7621 acactaaatt catctccgcc taaacgacct actatatcat catttgcaga actttcatca
     7681 agtgcctttg caacatcaat cagtacttta tctcctacca ggtgaccata agtatcatta
     7741 atcgacttaa aataatcaat atctataatc aataaagcac catttttttt agttgataaa
     7801 ttcaataaat tttgtgcagt taattttcta atatatgctg cattataaat attggttaaa
     7861 ctatcccttt gagccttatc taacaatcta ttcttttcct ctttttcaga atcaatatta
     7921 catattttcc cgattacata cattggtatt tttctatcat tataaattat ttttgaagta
     7981 attctaatcc agtgaaaagt atcatcaaac ataatagagt acatttcagt tgttccattt
     8041 tcttgtgatt ttattacatt aaaaaacttt ttattatatt ctcttcttat tttatctatt
     8101 tcatttgttg tattgccatt ttccccttcc ttatcatttt tatctaaacc ataataggtt
     8161 tcaatacccc tttctggcat agaaattatc aatttatctt gcttataatc atattcaaaa
     8221 aagtattcac ttgtcatttc atacaactga gtatgttttt tgagttccaa agatatcttc
     8281 tcgttcatac ttgtacgagt atgtaaacca aatgccataa atccaataat acaaattaat
     8341 aaactagcta caataaaaat agtctcaatt ggattctgtt gcataatata aagcaacgag
     8401 aaatcttgtc tatatgtagt attttggtag acaattgatt gcattttttc tgatgaaata
     8461 cttataatag ctttattaaa tatattcaat aattcttgtt tatctttttt aattatacca
     8521 aaacaaatac tctttgcttt ataagtttga ggaactagac gcacattttt atacttaggt
     8581 tgattcatat agtactgcat agaataacct tctccataag tgtaatctgc atagccttta
     8641 ttaacagctt ctatacactc ttctaaatta tcataccaaa ctatattctc ttcaaactga
     8701 ccatcatgat taataccttt ggttagagct atacgttttc catctagact attctcattt
     8761 aatttattat taatcatcat agtgtattga gtagaaataa aaggtcgaga catagctaca
     8821 ttatgttcac gagagtattc ataatcataa atcattcctg ctacaatatc aatttcatgg
     8881 tttttagtca tattttccaa atcttcttga gattttacag gcacaagttc aaattccaat
     8941 cctgttttat cacatatatg atttaacaaa tcaatagata tacctcttaa ttcacctgta
     9001 tctttatcta tattctgaaa tggtggtttg tttaaaagta ctcctacctt taatttttct
     9061 gtatctttaa tgtaattagt ttcttcatca gataaaaaca tttgattatt ctctggtgta
     9121 aagtatttct catataaagt agttgaaaaa taggggtcct tctgttcaat attattgatt
     9181 gctgagttaa gattttgagt taagtctgta ctacctttag gagtaatgaa ataaaacggt
     9241 cttggtgaga attttgctac agtacgtaca ccttctattt gattcatact cacattaaga
     9301 agtacatctg ctttaccatt ctttagtgct tctaattgct cgtcatttgt tttacagtat
     9361 accaattcag gtgacatcga attaatctta caatactcat ctaactcctt aagcctagtt
     9421 ttagaatttt ctattacagc aatacggatt tttttcacat cttgtgaact cattatgtta
     9481 tcaatatctg tattttcata taatgcttgt agtactgtat ttgctactcc ataactattt
     9541 cctgcataat tatatatttt ttgcattggc tcattgtata acatacctcc cattatatca
     9601 atttcaccat tttctaacat cttcattaat tttgtaatag attcatttat ctctccagga
     9661 acttgaacaa attcataatt ccatccagta tactgtgcaa tttctagtaa atattcatat
     9721 gtatacccat aatagttacc ttgctcatct atttcagtta aacctttttg aataggataa
     9781 gcaacacgaa tggtacgatt atcattacct atagcattga tatcaaaaga aaatatgcaa
     9841 gaagtgaata tagatgtaat tacaataatt aataaaagta atttttttaa attcactaca
     9901 caatcctcca tatagttaag atttacagta taaattactg cataaacttg tagacatgaa
     9961 aaaatagatt agtatataaa aggatatata aatctactta gaaatttaaa tatccttaat
    10021 atatacaata tatttaaatt ataactcatt tatatatcta ttacaacaaa tttaccctgc
    10081 taaataccta ctctatatcc cataatttta caagttgtag ctaagtttaa gttctatctc
    10141 atctcaaatt tagcagtgtt tctatttaca gttcttttat atttcacatc tggataaccc
    10201 aatataaatg aagtgataac cgcttcgttc tcgctcattc ctaacagttc ttttatcttt
    10261 ggctctacat ttgatgccat aacaaaaaaa ccattataac aaattcctaa tccttgagaa
    10321 taggccataa gttccatatt agatgcagcc aatcctccat ctacataagc acttccacct
    10381 agagatatat cacctacaac taccataagt agtggggcat cataaaataa agaatcattt
    10441 ccgttctctt tatatctttt gtacatcttc ttaaatgtat ttttatacat agaacgtaca
    10501 gggtcattgt catcagtatc taatgccaaa ttataaagac cttctattgc tagttccttt
    10561 acttctttta gtttttcttt tactaatatg tatcttatag gctgtctatt cccacctgtt
    10621 ggtgtgtatc taccagcttc cactatgttt tttatttttt cttcttctac ctctatatct
    10681 ttatattgtc ttatacttct tctaaattta ataaaattca ataacttttc gggttctaat
    10741 tcaaaacttt cacgattgta ttctattact tcatttttat caaatccttg catttctatt
    10801 gcatctgttg gacaaattgc catacagtga ccacagttaa tacagagaat atttttaacc
    10861 tttgctttat catccttaac ttttattgca tttacaagac agtctgattc acacatccca
    10921 cagcctacac atttttctaa atttatgtct atcattccaa attattcctc cttttaaaaa
    10981 atttacttta gtataaaaca aatagttgtc taagtcaatt tttatttttt aacaatatac
    11041 tagccttaac tacactttaa tggagaaaaa tatacataaa ttagaactta ttcaaaataa
    11101 aagataaaag aagcagatgt ctaaaagtaa actttttagt taatgagaca ttctttattg
    11161 tatgaaatca agtgtatatt tatcatttca acaataaaaa aggcttatct ctattttgag
    11221 ataagccctt ttctcaacta ctattgttta tcttaatatt atgttaataa atttaatttg
    11281 ctataacctt aagttacatt ttaatttgat ataactttaa attacatttt taagtagtat
    11341 ctttaaatta tattcttaaa atatatcatt aaactacatt ttaatcaatc taaaaactat
    11401 atcttaatta atcgaagaaa cttacattat aatcttttag tttcccatta tcatatacaa
    11461 tctttgcact aaagatattt ccagtcttag cccaatttaa gaacaacatg tcaccttccc
    11521 acatatttaa atcgaatatt ttatcattat caacccaaat taaatttcct tctttacaat
    11581 catttaaatc tttctcagat aattcacctt caaactcatc tgagaaatac aaaaacatat
    11641 accaatcttc ttcatcttta aattttggaa aagttatcaa tcctttatat gacaatgatt
    11701 taagagtaag accagtctct tcttttactt ccctaattac acattcttct ggagtctctc
    11761 cttgctcaaa ctttcctcca actcctacat acttaccttc atgtatatca tctttctttt
    11821 tatttctata caacatcaaa gtcttgtcat ctttttctat ataacaaata gttgtaagtt
    11881 tcatgtatct cgccttactt tctctttata ttctcaacta attattttca gcactttcaa
    11941 gcacatcact tatattcttc attgcattga gagaaagctc tgcaaattca tcaaatggaa
    12001 tccctatatc ttctatactt ctcattgcat ctctatttac atttgaagca aatctcgttt
    12061 ctttcattct tttaataaca gacttagtct ttacactggt aagtttttta tctggataaa
    12121 ctaaagctat agcagttata aacccagtta tagggtctgc tgcatatatt gctttttcca
    12181 ttaggctagt tcttgtaact cccattccat catgatgacc aagtattgct ctatacattt
    12241 cttcgtcacc gaagttttct tttttaagca gttctactgt tgttgtagca tgtccttcac
    12301 atatttctcc tggctttctt ccaactatat ctgaatccaa atcatgtaaa agacctgcca
    12361 ctgcccactc ttcttctttt tctggctcta gtcttttggc taagtccctc ataacagctt
    12421 caacagcata acaatgtttt ctcattctat cagtttctaa atacttaaat aaaatttcta
    12481 aagcttcttc tctcattttc actaatcccc ctgttttttc atatatatga tatctttata
    12541 tattaataca cataaattca agtttttata tatattcatg tatatttttc atgttatatc
    12601 tattctacac ctttatagta gtgtcctgca actgttccaa tttcatctaa tttcttatac
    12661 aagtctgtag catttttccc aagtttataa ttattactca atacctcaat gtgagcttca
    12721 ataacctttt taaccatctc actatcttca actgtaaaat ctatcctaaa tacatctatt
    12781 ccaatttcct ctaactcata tagattgtcc aacatacaag taaccttcga attgtaaatt
    12841 gtacttctac agaatatatc ttgtgttatc ctataacttg cgtccgtaaa gtctctcaaa
    12901 gcataattac tttccttaca tttagcaact ctcttatcct ttttacaatc tctaacaact
    12961 actcccatag gacagtattc tgtaaccatc ataggagcat atccatatat taccgcttca
    13021 acttctacat ctgtatattt taaagtttgt ttaatttcgt ttaagtttag ttcttgtgac
    13081 aaacaaactg tacttgctcc ctcttctttg aagtgattta tactttcact attaaatgca
    13141 tttaagtagc aatctatatt taagtctttt ttcttaaagt attccataga gccccagtta
    13201 ccaatttgaa ccttattaat tccttttata ttatccaata tctcaaattg cttgtactct
    13261 ttatttctaa caatccttgg tgctgaatag attaattttt tgtcagtgac tgctgtatta
    13321 gagtgattct tattcaaagt atctatttta cttgtatttg ataaagtatc tatgttattt
    13381 gtatttgaac ccattaattc tttaggaata cctaaagacg aagaagtatc taaatccata
    13441 gaaatacttg aatctgtaga aatatttgag accatttgta aagcttttga taatgtgttt
    13501 atatcttcat aatagattaa atccacatca taacttaata ctgcttctaa ttgctccagg
    13561 ttcttaactt taactctaat cttaggaact gtatcaagtt ttttctgttt atttactcta
    13621 tttggctggt attttaatct attcttctta aactttctat cttttatctt tattctttct
    13681 tcatctaaaa gctcaataca atctcttctc atttgattta acacacttat aggcatactt
    13741 acaccttcat ccaggtcaat ttctatctct tttaacttgt agggtgtatt tccaagtttt
    13801 tgaatttgtg tttctatttt ttctttactt aaagccacct ttatagcttt ttcaactatc
    13861 ttgtctcctt ctaccaccga tttatttcca caaaaatcac ttataaaaag ctctggtttt
    13921 ttacctagtt taatactcac ttttgcactt atattagttt ttatgaactc tttatcttgc
    13981 tgaaatgtag cttgtactct acccattaat tcgctatcag aagtcttata tataatctga
    14041 tttctctttg cttctcctac aaaatctaat tctatcaaat cccctttatg tgctattgta
    14101 gttatctctc cattctttat tatccttcca atagtacctc cacctaagtt aattccatca
    14161 ccttttttaa gcgtattttc taacattact ctaagtcttt tgttctttct attataatct
    14221 aaaacttttc caatatatag cccttgatta ttaggtctat tagagttcat tatatcctta
    14281 ccaacttcac ctaatatata tccttttgta aactttctat taaatatggt atataaatta
    14341 tttattattt cctcagaaat acttattttt ttattatcta caaattcatt aatcgcttct
    14401 ctatatcctg aaaccacagt agctacatac tctggctttt tcattctccc ttcaattttt
    14461 aaagataata cacctgcttc aattatttta tccaaatctt ctatagtact taaatctcta
    14521 ggactcaaca aataatctcc attacttttt actacttctc ctgtatttat atctattaac
    14581 tcatactttt gtctacatgg ctgtgcacac ctacctctat ttccagacct attgcctatc
    14641 atactactca taaggcattg acccgagtaa catacacata aagctccatg tacaaaaact
    14701 tctatatcta cagttgtatt ttcacaaatc cattttatct catcaacatt taattctctt
    14761 gctaaaacaa ctctatcaaa ccctacactc tgaaggtatt ttacatcttc taaggaatgt
    14821 gctaccattt gagtgcttgc atgtagttca aagtcaggta atagtttttt tataagtctt
    14881 gccattccta tatcctgtac tattaaagca tcaatatcta tatcgtataa aaattttaca
    14941 tactctacaa aatcttctat ttcatactgt tttatcaatg tatttacagt aacaaagact
    15001 tgcactcccc ttatatgaga gtatttaaca gcctcaatca gctcattttt atcaaagtta
    15061 tttgcagatg ctctggcact aaagtcttta ccacctaaat acacagcatt tgctccgttt
    15121 tgaactgctg cttttaatga atcaaaagag cctactggtg caagtaattc tatttttttc
    15181 atatttttct cctctattta tacttgttta ggttaatata attatatcta taacttaaat
    15241 atttatatta aatttatata ttgattacta tattttaaat tcttcaatta atttatatac
    15301 tgcttactaa atttaaaaca ttttaattaa tttgtacact gtttactata ttttaaatgc
    15361 ttcgattaat ctatatattg cctactatac ttaagacatt ttaattaatt tatacactgc
    15421 ttactatact taaaatactt cagttaatct atatattgcc tactatactt aagatatttt
    15481 aattaatttg tacactgttt actatacttg aaatgcttca gttaatctat atattaccta
    15541 ctatacttaa gacattttaa ttaatttgta cactgcttac tatacttaaa atacttctat
    15601 taacttatat attgcttact atattttaaa ttctttaatt aatttatata atatttgcta
    15661 tatataaagt atatcaaata aaagcctcat ttttttgtaa atttacagaa agttaatttt
    15721 cattaaatga atcatcttta ttgataaaca taattccaaa ggccactgcc aaaataaaaa
    15781 aacatattag tattgatttt tccacgaaaa tttttgtaaa aaaagttcct aaaatagctc
    15841 cagatataaa gaatataata atagcataat actgtaaact cttttttatc ataaaaatat
    15901 tcttagtatg tatataatga aataaagttt ctgtagcact tcttaaattt ccagtacaca
    15961 ttgtcgttgc aaaactattc ccatttactt ttctaaaact ctctacttgc attgaacaaa
    16021 taaaagaaat agcaacattt acaaacatat taaatcttcc tctaggaaca aatgaaacaa
    16081 taagtattgt aatcatttca attataatga taacctgacg ccaatgaatc tttgtcttat
    16141 tatttgtaaa atactttctt atcttctcgg ctataaatac acctaacata aatgccaaaa
    16201 taggaatcaa ataataaaat gattccttta cattaccttc tgctatatta agcccaaata
    16261 aaactatatt tccagtttgt gcattagcaa agactttacc tcttaccaca taagtataaa
    16321 tatccaaaaa tcctcctgtt attgccaaca aagaacataa aaagaatgat tcagacatct
    16381 gactcttgct tgtcatatat aaacctctca ctttctaatg atagatacaa aatcaaaatc
    16441 attttaaatt ctatttttca ttgatataaa aaaattattt atatttaaat attgtctata
    16501 tttatacatc atttatattt aaacattatc tatatttata tcatttatat ttatttatat
    16561 ttaaacatta tctatatcta tatatcattt atacatcatt tatatttaaa cattatctat
    16621 atctatatag catttatatt tatttacatt taaacattat ctatatttat atagcattta
    16681 tatttataca ttaaaagaag ctgtctcaaa aaacaagaga cagcaaatct tttagtttat
    16741 tccacatttt ttgcatcatt atttaaccct gtacatttta actgtaaatc ataagctttg
    16801 ttttgaaact cagaagctaa gttttcagct atagtagctc tttcatttgc ttcttcaagt
    16861 tgagtactta actcctcaat cttgttttta taagcatcta actctgctct agaaccttct
    16921 gtcgtattgc taagttcgaa tatttcttgc tttttatttt ctataatctc aattaactct
    16981 ttcatcttag tttcagcttc acgattttct gcttcttttt ctgctatcgt aagctttaaa
    17041 gacttgattt ctaattgaag ctcttcatta gatgctccaa ccttcttact caattcttca
    17101 ttagctttaa tcaacttctc attctcatct gaacattcaa ataaaaggtc tgatatattt
    17161 aatgcagtta atatcgcagc aactgataag ctcaatttcg ggttttgacg tgttatttta
    17221 tccatttctt tgtctacaaa atcagcaata ctaatcataa acttttctga tttgtctcca
    17281 accattggat attcagcgcc atggatttta accattactt tgttcatcct aacctccccc
    17341 gacattagtt gttaatttga tcttaagtta gcatttaatt tttcgcttaa ttcacttaat
    17401 atcttatcat gcaccttagc tacatcttca tctgttagag ttttatcttt acttctataa
    17461 gttattgaat atgcaattga cttgtatcca gcttctattt gagaaccttt gtaaacatca
    17521 aataacttgt agctttctac taatccttga ccattttcct ttattatatc ttctatttgt
    17581 tttacaaata catcatcttt aacaataaga gctatatctc ttgatgtaga tggatatttt
    17641 ggtaatggca cataattctt tgttctatca gaattatcaa atacaaagtc aatatctatt
    17701 tcagcgacat aaactctttg acctagattg tagttttcta taacatctgg atgcaattca
    17761 cctaatgtac ctacatattt attattatac actattttag cacatctacc agggtggaag
    17821 gtagtattat tatcttgagg ttcaatttca taacctttaa atccgacatt tacaagtata
    17881 gtttctatag ttccttttag tgcaaagaag tcaacatctt taccgtacat acctacacac
    17941 attctatttt cttccttagg taattctgaa tcttgtggga tgaatatatg tccacactca
    18001 aatgcagata cttcttctat tttatgagat acattagttg ataaaacatc taacatattt
    18061 ggtattaaag tagttctcat tacagaagtt tcttctccta atgggttaag taatttaaca
    18121 aaatttctct ttgcattacc ttctggtact cttatcttat ctactccctt agggcttaca
    18181 aatgaatatg ttaatatttc gtttaatcct acagcagttg aattatcttt taaggcatcc
    18241 atgaattttt gttttgaagt tttaattcct gctgtcgtat ttccttctaa ctgtacagaa
    18301 ggtatattct caaacccata aattctagct atttcttccc atacatcagc ttcttgttcc
    18361 atatctgttc taaagcttgg tacatctatt tctaatttat catcagctac caaattacat
    18421 ttaaactcta atgattctaa aattcctaca aactgctcca ttggtacatc tacacctaat
    18481 aaatgattaa ttctttgagg atttactacc aattttttag gttctggttt atttggatat
    18541 acatctacaa caccttttaa aacttttcct gctcctaaca tttcgacaag ttgtacagct
    18601 ctatttgctg ctatctctgc aagattaggg tctaaatctt tttcgtttct tgaagatgct
    18661 tctgacctaa taccaacttt tttagctgtc attcttatgt tttctggttt gaaattggca
    18721 ctttcaaaaa gtacagtctt cgtattagaa gttatttctg agttagcacc acccattaca
    18781 ccagctaaac caagtgattt ttctccattt gttataacta gcatatcttt gtctaatgtt
    18841 ctctcaacat catctaatgt tacaagtttc tctccatctt tagcatttct tactactatt
    18901 cttccagtct ctacttgatt tatatcaaaa gcatgaagtg gttgaccaag ctctaacatt
    18961 acgaagtttg ttatatcgac tatgttactt ataggtctta ctcctgcttc tgtaagtctt
    19021 ctttgcatcc aatatggaga aggttctatt tttacatctg taaccattct agcaacatat
    19081 cttctacata aatctggatt gtctatttca actttgaagt ctatctcttc atcactttct
    19141 tttacttcga tttcaggata ttttaccttt gttcctatag ttgcagctgc ttctctagct
    19201 atacccatca tacatttaca atcaggtctg tttgaagtta attcaaaatc tattaaagca
    19261 tcttttaatc ctaaaacatc ttttatatct tttcctaatt cataagaatc ttcgtggtct
    19321 aaaatatata taccacctct tttatactct tcaatgtaat gttcatctat acctagttca
    19381 gctgctgaac acatcatacc atctgataat tctcctctga aatcagtctg tttgatttca
    19441 actcctccag gtaacttaga accatttata gctactggaa tataatctcc ttctgatata
    19501 tttgtagctc ctgtaactat ttgaacaact ttatctccaa tatctacttt agttacaacc
    19561 aacttatcag catttggatg ttgttttatt tctaaaatct ttccaaccaa tatattttct
    19621 atttcttcac cataataatc tattgtttca actttagttc ctgtcattgt cattttatca
    19681 gcgaactctt ttacatccat gtctatatca acatagtctc taagccattt taaagatact
    19741 aacatctata taccctccta attaaaattg atttaagaat ctcatatcat tttcgaataa
    19801 taatctaata tcatctattt cgtatttaag cattgcaagt ctttcaaccc caactccaaa
    19861 tgcaaatcca ctgtaaactt ctgggtctat tccacaattt ctaagcacgt ttggatgaac
    19921 catacctgaa cctaatattt ctatccaacc ttcatattta cacattgggc aacctttacc
    19981 accacatttg aaacaagtaa catcaacctc tgcacttggt tctgtaaatg ggaagttgtg
    20041 aggtctaaac ttagttttga tatcagaacc aaacaatttt tcaacgaaga tatccatagt
    20101 tcctttaaat tctgccatag taacatcttt tccaacaaca agcccttcta tctgatggaa
    20161 cattggtgag tgtgtagcat ctggcgaatc tgacctaaaa catctacctg gtgcaattac
    20221 ttttattggt aactcttgac ttctcatagt tcttacttga actggagatg tttgagttct
    20281 aagtaatacc ccatcattga tatagaatgt gtcgctcata tctcttgatg gatggtcttt
    20341 aggagcgttt aatgcatcaa agttgttttc aacagtctca acttctggcc cttctgctat
    20401 agagaatccc attccgataa atatatctgt tacttcatct ataatttgag ttattggatg
    20461 tttcttccca acctttaaaa cttttcctgg ttgagtaaca tctattactt cctcttttaa
    20521 ttttctttct ttttcaatag catttatttc ttctttttta gaatttatgc taagttcaat
    20581 gttttctctt acctcatttg caaccttacc aactactggt ctttcttcag cagataattt
    20641 acccatttct ttaagtatgg tagttatttc accttttttt cctaagtact taactcttaa
    20701 actttctaca ctttctatgc tttgtgcttc ttttatttca gccaaagctg cttcacgtaa
    20761 agcaagtaat ttttcttgca cacatatcac cctttcgttt ttttgtataa aaaaagcccc
    20821 tcaatccctg tctagggacg aaaggctata gtttcgcggt accaccctag tagtttaaca
    20881 atttaaaatc atcaaaccac ctctaacaaa cttaacgcgt ttttgacgtc aaagcctact
    20941 gtaaaatttc agctctgtta ctccagaggg aacttctcat gacctcttag aaaacactcc
    21001 cagcctaggt gtttatctct ttactaagtt tagtcaaaat actttcctct ttcattgcat
    21061 ttacaatatt atattgttta ttttagtatt atatcatttc cattcaaatt atgtgactat
    21121 ttttaatata tatccaatac ataattaatt taattatttt ttatataata ta
//
LOCUS       DAEMAE010000049        21028 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEMAE010000049 DAEMAE010000000
VERSION     DAEMAE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.050"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(114..974)
                     /locus_tag="KPH93_001453"
     CDS             complement(114..974)
                     /locus_tag="KPH93_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG1688282.1"
                     /translation="MAMKWRDYADDINLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1530..2858)
                     /locus_tag="KPH93_001454"
     CDS             complement(1530..2858)
                     /locus_tag="KPH93_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1688283.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANRNYWPELN"
     gene            complement(3006..3335)
                     /locus_tag="KPH93_001455"
     CDS             complement(3006..3335)
                     /locus_tag="KPH93_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1688284.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3359..4648)
                     /locus_tag="KPH93_001456"
     CDS             complement(3359..4648)
                     /locus_tag="KPH93_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1688285.1"
                     /translation="MEALQNNLRKFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4664..4990)
                     /locus_tag="KPH93_001457"
     CDS             complement(4664..4990)
                     /locus_tag="KPH93_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688286.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(5125..5754)
                     /locus_tag="KPH93_001458"
     CDS             complement(5125..5754)
                     /locus_tag="KPH93_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688287.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5969..8626)
                     /locus_tag="KPH93_001459"
                     /pseudo
     CDS             complement(5969..8626)
                     /locus_tag="KPH93_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(8985..9929)
                     /locus_tag="KPH93_001460"
     CDS             complement(8985..9929)
                     /locus_tag="KPH93_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688288.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMDKVYEMLNLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKARNRIEYNSN
                     VYDCSNEKSLSWIEISDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9922..10947)
                     /locus_tag="KPH93_001461"
     CDS             complement(9922..10947)
                     /locus_tag="KPH93_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688289.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSGDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(11022..12590)
                     /locus_tag="KPH93_001462"
     CDS             complement(11022..12590)
                     /locus_tag="KPH93_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1688290.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGILPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQVTPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12703..13614)
                     /locus_tag="KPH93_001463"
     CDS             complement(12703..13614)
                     /locus_tag="KPH93_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688291.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13598..14584)
                     /locus_tag="KPH93_001464"
     CDS             complement(13598..14584)
                     /locus_tag="KPH93_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688292.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14944..15345
                     /locus_tag="KPH93_001465"
     CDS             14944..15345
                     /locus_tag="KPH93_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1688293.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15348..15746
                     /locus_tag="KPH93_001466"
     CDS             15348..15746
                     /locus_tag="KPH93_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG1688294.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(16074..16298)
                     /locus_tag="KPH93_001467"
     CDS             complement(16074..16298)
                     /locus_tag="KPH93_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG1688295.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16333..17700)
                     /locus_tag="KPH93_001468"
     CDS             complement(16333..17700)
                     /locus_tag="KPH93_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1688296.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17784..19319)
                     /locus_tag="KPH93_001469"
     CDS             complement(17784..19319)
                     /locus_tag="KPH93_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688297.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIAEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYELMVSRKLNTNSIAYNDTLMELSSFFKSYIIKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNLYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFILNKKTETTLWKIEYIKFMKKQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19342..19776)
                     /locus_tag="KPH93_001470"
     CDS             complement(19342..19776)
                     /locus_tag="KPH93_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688298.1"
                     /translation="MISPIILSSINRNLKEIERNELLETNIESGDYGLTLSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLETINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(20035..20946)
                     /locus_tag="KPH93_001471"
     CDS             complement(20035..20946)
                     /locus_tag="KPH93_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG1688299.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMYKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT         6650 a         3745 c         2185 g         8448 t
ORIGIN      
        1 atttattaaa aatacaaatt atattttatt aatttttact acttgaaaat tattatctta
       61 tacaatgaat ttgtataaga taataagtat ttttaggatt ctattacata atattattta
      121 aatattcttc tatatttctc ttcttcctct tcagtaaaca cattaaatac aacttttaaa
      181 tttttttcag gattttcttt tagccataat ctaactctac ttacagcaag attagctgct
      241 tctttttttg gatagccaaa tacacctgta gaaattccac aaaaaacaat attttttatt
      301 tcatcaattt cttttattgt atttaaacat gatttataac aatgaagcag ttgcttttct
      361 tgctctttac ttggctgacc accagataca ataggtccaa cagtatgtac tacgaatttt
      421 gcaggtaaac aataacctct agttatctta gcatctccag tatattctaa atgcccttgt
      481 ttttttatta ttttgtcaca atcttctcta agtctaggac ctgcacatga atgtatctca
      541 ttatccacac atagatgtaa tggttgtaaa caccctaata acttgttatt tgcagcgttt
      601 actattgcat ctgctcttaa attcgttata ttacctctcc atatggcaat cccttcctct
      661 acttcttcta tatcatttac atctactaac tctctttctt cacattcaat caacaataaa
      721 ttatgtaaca tattaagtat ctctggagca atttcttttg gtggatatac attcaaaagt
      781 cctctaagta tatctctagg cttaatttca tcatttgata aattgtatat tttagaagga
      841 acttcttttg aaaaatatgc aattaaagtg tttatatttt ttcttctttg ttcgtcagtt
      901 agaggcttga tagttttatc aaaatcctca aataaattta tatcatcagc ataatctctc
      961 cacttcatag ccatctctcc attcttgtat tttagactta aaaataatta tgtatttatg
     1021 tacatattta tttatttcta taaacaccta cataaatcct gttaaaagtg taaatttttt
     1081 attatgcaac ttctcgagtt atttctattg attgatagta aattattgta cctaaattta
     1141 atccaactaa caagtaacta aacctacttt cctaactgca aaatacacta tgtttgccac
     1201 ttgtatctca tttgtaaata aataaggata ctacttgttt accaagtaca aaacttataa
     1261 gtggttgtac tcctagtgtt aatccaaata aagtcatata tatactaata cttatataat
     1321 ttattatact ataagctaca attcctattt tcctcataat ttttactaaa actacattgt
     1381 gtattaatgc tataaataac agattattta caagtcttga agaagtcaga gtatcatgga
     1441 caaacttata aaatcataaa taaagtccct acaaatacat taactaagct atgtactaaa
     1501 ctgtaggaac tttaaaaaat atattattct taattaagtt ctggccaata atttctattt
     1561 gcttctatta aatcatctag taattctttt gcaaccgttg cactaggaac tgtcttagat
     1621 agcataagtg cttgccacaa tttattatac gacttctcaa tccaagcatc aactactaat
     1681 ttttcaactg ctaattgttg ttctattaaa cctttttgga atgttggtat tttccctatt
     1741 gataatggtt catacccatc ttttccaact atacatggaa tttcaaccat agcatcatct
     1801 tgtatatttt ctatagctcc attattttga actatcaata acatacgctc ctgtgtatta
     1861 aaagctagag ctgtagctaa atccactata aactctgcat gtattcctat cttaagccca
     1921 ctgcccttgg aatgtccttg cttttctatc aatgcacatg aaccaaaaac ttctttttct
     1981 cttccatcaa taacttcatt ggcacgagta taatttacat cagaatgatt tacaacataa
     2041 tctggaaata gatagtactt taagtaacta cttggtattg tagttaaatc tgtttttact
     2101 atttctttag taaataaatt agtttcaaac caactatctt tcttgtcatt tttcattcct
     2161 tcttcaccat ttataaaacc atattctttt atatgttcta ctaattttgg taaaagttca
     2221 tttccttttt tatctttaat tgaagtatac caaccaaagt gatttaatcc aaagtatctt
     2281 atatccatat gtttacgaga tttaagcccc gcaatccttg ctatattatg ttccatacct
     2341 attggcatat cacaaatatt tattacccta gaattaggtc tcaaccttct aacagcttct
     2401 gctattattg atgctggatt agaataattt aacatccaac aatttggaga atacttttcc
     2461 atatagtcta tgttttcaag aacaccttct atagacctca ttccataagc tattccacct
     2521 ggtccacaag tttcttgacc aactacccca tgtcttagag gtatcttttc atctagttcc
     2581 ctcatttcaa gttttccaac tcttatatga gctaaacaaa aatctacatc tgtataagct
     2641 tctttagggc atgttgttgc taaatactct atattagggt ccttttcctt tattagtatt
     2701 tcacaagctt tagctacctt attttgtctt tcttcatcat tatcatataa tttgattgct
     2761 cttaatggta acctatctaa gttatctagc aacattaata tgatttctgg ggtatatgta
     2821 cttcctcctc cagctattac tactgaaaat ttttccataa taatatctcc ttttctattt
     2881 aaaaatttac tatatttata aataaattta taaatactag tactttaaag taaaagggag
     2941 ttcatttatt aaatctatta atttaataaa ctatagccaa acttcaagca ctatttacaa
     3001 gaaatttaat tcttattaat tcttgggtcc atcatctcaa atatatctac aagttcagat
     3061 actaaagaat gagcaaataa tgcatttgaa taatggtctt cagagtgcac aactaaaaaa
     3121 tcagtcccac tatcttcact attcatagca tttgatagta attctaaatg atgctttgat
     3181 gctgtttgta ttttctctga ggctgaattt aaaaggtgtt ttgctttatt gtagtcacct
     3241 ttttttattt ccttaagaga catcattaca tcacttttag catctcctgc ttctgatatt
     3301 atactaaata ctttttcttc catttcaggt accatatcct tctccttcta atgacttgtt
     3361 aaaaatcaaa gctatcatca tctaatgttg tagctaattc tgcattttta tttaattctt
     3421 ccttctccat tatcttaaag aatggataat atatcaaggt cataagtatt atacttgcaa
     3481 atactaatac gattgccttc caatctaatg tcatcaaaaa tgctgcaacc cctggaggta
     3541 aaaagcctgg tggttctgca ataggtaatc ctactaatcc tacttttaat gccataaaat
     3601 taagtgctcc aaccaataca ggtccaaata caaatggtac aaacatatat gggttcataa
     3661 ctattggtgt tccaaatatt actggctcat ttattccaaa tagtgatggt aaaatcacaa
     3721 cttttcccat actattaaaa ctcttacact tacttcttga cataaggtac actaatccca
     3781 aagttattcc tgaaccagtt acttgcaaga aaccatagta aaaacctatt gtaaacacat
     3841 attttgggtc tcctcctgct gctattaacg ttgcattttc tgccatatat gtagctgcta
     3901 ttggcatcca tatacttgaa gttattgctg ctccatgaag cccaaagaac cataataatt
     3961 gagttataaa tagtattata gttactgcaa aaacattatc tacactgctt atagctggtg
     4021 ctaagaaatt cattataata gctggtagtg gttgacctga gacattttta gttattgctg
     4081 ctattgctgt aaataacatt atacatacta tcattggagt taaatttcta aaacttttat
     4141 ttactacact aggtacttct tttggaaatc taattacgag atttttatca ttcataaact
     4201 tagtaatatc aactactaaa agtgctacta atatacttac aaataaacct tgtgcatcta
     4261 aataacttgt atctaataca ccatctacta cttgtccaca tacaacaagt tgtgcaacaa
     4321 ctgctgtcat acctatttct acatgattaa gattatattt tttagctagt gcctgagcta
     4381 taaaaaatgc tgaatatata gataataaac tcatagtaaa tttattaggg ataagtaatg
     4441 tatccatatt ttcagataat aatgtttttg cacttccttc aggtaacata ttgtttaatg
     4501 ccataaataa catacttaaa gaaccaacaa ttattattgg ggtaatgctt atcatacctt
     4561 cttttatagc ttgaagatgc ctttgttttt caatcttttg tgctattgga agaagaaatt
     4621 ttcttaaatt attttgaagt gcttccattt ttattccccc caattatcta tttttatata
     4681 attcgattgc cgacttcata acagaaggag catccatacg accataatgc ttagtatcta
     4741 ttaaaccaac tgctattttg taattcttac aagcttcaac tacatcatct tccttaaacc
     4801 taatttgtgg tgctacaagt actacatctg tatcattagc ttcaatgtat gattttaaat
     4861 tttcaaatga tgttgcctct atatttatag ttttagtttt taacttttca tgctcactac
     4921 aaaaactttt catcttttcc acaagtacac ctgttgaagc tccatttgca catactaata
     4981 aaatattcat aattaccctc ctacaatatc taacctcttt tgttttctaa ttttttatca
     5041 gccaattctt actctaattc ctatcagttt ttactttaat tcttattact ttattttaat
     5101 tcttattaat atttactcta attcttagta ctgccaatga ttaaacttga aagctgtatc
     5161 tataacttct tcttgcttat atttttctaa tatttttaag tatctttctt tataatcatt
     5221 atctacttct ttttctggta ttactatact agcttcattt aaaaagtgga atgaatccct
     5281 tcccatttct aatcctctat atgtatgctt gtcaagtgcc acgtccataa cttctggaac
     5341 tttacccatt gcaaatcctt ttataattat attttttaat aaatcacttg atctatcttt
     5401 ctcacaagca caaagatatc ttattgcatg tatgaaaaat aaaggtctgt ctccatctgc
     5461 atatgaatat tcctttctta cttgatttaa attatttatg attattacag ctgatgggtc
     5521 acccattcct atatcttcaa ctgatattgt aagtattctt ctccatagct tatcttctaa
     5581 ttctggtgat gatatatata gttcataagc aaattcacaa gcttcttcca ccatatttct
     5641 tcttatagct ttttgaaatg acgatattat ttcgtctgca ctaaatccgt ttcttgtagt
     5701 cattcttgcc catgggtcat ttgccttttt atctcttaat attttatctt gcatattatt
     5761 tactcctttt tatttaaatt ttaattattt atattactca tttaataata taagcaagta
     5821 ttatgccagt ttttttagac actttaaaac actgttattt caatcatttt tatcatcaaa
     5881 acttagatac tttgtctaag tatatttata tgtgtctaat attaatatta ttttagacta
     5941 aaaaaaatta ccaaataagt ttttatactc attggtaatc tttttcgatt atttctgcat
     6001 attctaatat taataatatt tgatagcact ctttttcatt aatttctata ttatattttt
     6061 tatctaattg agatactata tcttttattt tgcttaggct cttgtgataa tcaagtgttt
     6121 tttctttatc aagtttttca atttctctag tatttccaat caacccatct accatgaaag
     6181 ctaaatgcat cataagccca attactttat cgtatttctt ttctatatta aatactaaaa
     6241 acaattcatc agacacatat tttatatctt taaatatcct attataatta gagcttttta
     6301 aggatgtctt taaaccttca actacatcat cataacctat acttatcttt ataatatttt
     6361 ttaatttatt gatattttca tccttatcta tatctttttt gctaatatag tcaattcctc
     6421 taatattaat tctttgttca ttgattactg caacaatatt actactttct ttaagttttg
     6481 taatatttaa acttaaatct ttttctgttt tatatgggac agatataata tctattccat
     6541 ctaagtacct actcatcttt tcttctaata tttttcttgt tttattatct acaccttctg
     6601 aaacatgtga tgctataata attgttttag acaaattttc tttattttga atttcattag
     6661 ttccaaaata gtactttctc gtgttagtga tagagttgta tacatcattt aaaggtaatc
     6721 ccatattggc cttctgaact gcttctaaca cagttaatgt cgtaaccata ggaattactc
     6781 ttactccaca ctttttttct ttctttataa tttcatcaaa ggagtttaat gaacccatat
     6841 ctgtaaatag taatacatct ttattgtcta tattattaat taaattttta aaatctacaa
     6901 tcacttcatc ataactctta tctaacttca tatcgaaaga aatagcgctt cactttccaa
     6961 taatatcacc aactgcttta accatactac ttgctgtagt ttctccatgc atagcaacta
     7021 taatattaat gttatttact attttatttt gttcatcttg tttagcttgt actatgaata
     7081 atgtaatata tgctacagtt tctatagata aaaataaatc aaattcattt tcaattattc
     7141 caactatttt taatgctgtt ttaaattcac ttctgttatt tcttctaatt tgattcaaat
     7201 ctatgttgtt tgtagagcta cttttatcca atacgctttt tatataaatt gccattgact
     7261 taaatattga ttcacttata tctatttctt catcttcttt aataatttgt cttattgatt
     7321 cacacatgct tttaaactta acattattaa atagagaagc atctgctata ttcatttcta
     7381 cagatttatt aagattagta tttactgcat taataatatt tatatcattg tagttaataa
     7441 cttcttcttc tcctaatgac gtaaatacaa aatattctat tccatgtgtt ataacttcat
     7501 ctgctttaat agacttctcc ctataatcat cactcacttc atttatagat ttatcaaaat
     7561 attttttctc tatataaact ggttctttat tatctctttt atatcttagg tatccatttg
     7621 caacagctaa ttttatatca ttttgaagtt gacctacatt atttttacaa tcataaagta
     7681 atagtgattt aattatttct ttatgtactt ttattgaaat agaaatactt tttgattctt
     7741 ccttgaaaaa atttttgact aattccattc tttcaatcat tgtcctatct ctcagtgatg
     7801 gtatttctat tttcattggt attcttctat tgaatgtatc taatagtgtt gatgtcggat
     7861 tttctgtagt agcacatatt attaatacct tagactctct ttctttcgtt gcatctccta
     7921 gtcttttaaa tgttccctta tctataaaag taaacaacat ttcttgacct tctgctggta
     7981 gtctatgaat ttcatctaaa aatagtatcc ctccatttgc aatttccaaa agtccttttc
     8041 tggaagattc tgcacctgta taagctcctt gttctactcc aaaaatatgt cccataagta
     8101 attgtgcatt attagcataa tcagcacaat taaatgttac aaaaggagct ttattttttc
     8161 taattccact atctatagaa taatcgtaca tatatctagc aatcatactc tttcctactc
     8221 ctgtttctcc atagataata gtatgaagtc cattcgtagg atagagcatt gcaaccttgc
     8281 atttttctat tataggtctt aaactatgtt ctttacctat tagtgtttca aaactatctt
     8341 tttttatttt gttttctact tttgttcctt tttttattcc aagcaactca ctttgcgtat
     8401 caagcaattc attcttctta aggttaagat ttgcagggat ataatacaca ggtcttcctt
     8461 ttatctttgt aaccataccc tcattgaata actggtttaa atatgtactt atattgtttc
     8521 ttgcaattcc tgtcttttct gataattggt tagctgtaat ccctttttca ttttccctat
     8581 atgatgattc taaaactttc attactttat ctttcttata tatcattaca agctccccta
     8641 cattataata ttttattaac taaaacccct atacacactt tattttttaa taaactctta
     8701 ttctttaact aaggttataa tttatcaaac tttccttact ttaaattata gaaaattatt
     8761 gtagaaaaaa taaggttatt gctcattttt tctacattac tatttttaaa tttatagtac
     8821 tttcttaata acatacttta cttgttttta attttattat atttgaattt attatatttg
     8881 aaaagtagaa ataaaaaacc ctatttggga gtagttttaa aacttctcca aaaatagggt
     8941 tttagtttgt atttcaaata aatacatgtt caagtatttt tatactaaac aacttttaaa
     9001 ttttgttggt atttatttat gtctgattta gaagaatata caaaatgtcc atctgaaatc
     9061 tcaatccaac ttaatgactt ttcatttgaa caatcatata cattagaatt atactctatt
     9121 ctatttcttg ccttagcata tctagggtca ggaacaggaa ttgcagaaag tagtgacttt
     9181 gtatatggat gaataggatt attatacaat tcatttgaat ctgccaattc tactaatttt
     9241 ccattataca taactcctat acggtcacta atatgtctca ccatagataa atcatgagct
     9301 ataaataaac aagttaaatt cttttcttgt tgcaattttt taaataagtt tactatttgc
     9361 gcttgtatag acacatccaa agaagctata ggttcatcag ctattataaa ttctgggtct
     9421 actgaaagag ctcgtgctat gccaatacgc tgtctttgtc cacctgaaaa atcagatgga
     9481 tacttatttg cataacttct atcaagtcct actagattta gcatctcata tactttatcc
     9541 attctatctg ttttattttt acagatacct tgaattttta gtggctctga aattatatct
     9601 gcaattgtca tacgtggatt taatgatgaa gtagaatctt gaaaaataat ctgcatactt
     9661 ttgtttacat ctttttttat aaatttgtac gtttttttat ctgatataca gtttcctttg
     9721 taaattactt ctccaccagt agattcatgt agtcttatta tagttttacc agttgtagat
     9781 tttcctgaac ctgactctcc taccaatcca aatatctcac ccttatatat atcaaaactt
     9841 atatcatcaa ctgcttttac agtagtactt ttgtccaaat gaaaatattg ctttaagttt
     9901 tttatttcca gtattttttc tctattcatt agaaatcacc cttccacaat ttactctaac
     9961 tggtacatct acttcaggtg catctgggtg caataaccaa gttgctgcac tatgtgtatc
    10021 attaatttta aacattggtg gctctttttc ataatctatc ttaagtgcat ttttattcct
    10081 tattgcaaaa gcatctcctt ttggtgggtt caataaattt ggaggcattc caggtatatt
    10141 atataaatag tcatcctgcg aatctaaagt aggtaatgaa cctaacaacc cccaagtata
    10201 tggatgtcta gggtcataaa atatatcctc tacagttcct atttctacaa tctttcctgc
    10261 atacataact gctattctat cagctatagt tgcaactact cctaaatcat gagttataaa
    10321 tattattgac aaacctattt tatgttgtaa atcctttatt aaatcaatta tttgagcttg
    10381 tatagttaca tctaaagcgg ttgtaggttc atcagcaatc aatacatctg gattacaggc
    10441 aagtgctata gctattacta tacgttgtct cattccacct gaaaaatgat gtggaaattg
    10501 tttaaatctt ttctcaggat tatctattcc aaccaattca ataagctcta tggctctctt
    10561 ttttgcttca cttttactta taccttgatg aatgattaca gcttctgcaa tttgctttcc
    10621 tattgatata gttggattga gagatgtcat agggtcttga aatatcattg atatattctt
    10681 tcctcttata tcttccatct ctttttcaga cttcttaact aaatcacctg atttaagtaa
    10741 aacctctcca tttttaatat atccattgta tgggagtatc ctcatcaaac tcttacacag
    10801 tacagatttc ccacaaccag attctccaac tatagccaat gtctcacctt ttttcagatt
    10861 aaaagataca tttcttacag cctgaacttc tccttcttct actttaaaac ttacagatag
    10921 attattaacc tccaataaat gttccaaaaa ccatcaaccc ctatgctatt caaaccttag
    10981 ggatatattt ttatccctaa ggtttctaat taatttcaat attattcaat tgtccagtca
    11041 gctatgttcc agaatatacc tacaccatga tgacctaaaa ctgtatcagg tgttaatcct
    11101 tttatgtttg gttttccaac atatattgca tctatataag caataaatgt ataaggcata
    11161 tccttagtca tttctacttg gaattgtttg tataacttta atttttcatc tttatcttct
    11221 gtttctcttg ctttttgaag tattttatct atagtaggat tagagtaagc actgtaattt
    11281 gcacctttat ctgttccaaa tactttatat gtatggtcat ctgggtcaaa tggacttccc
    11341 caacctatta aatgagcatc ttgattagcc caatctgttt cagttacaac aacagcttta
    11401 gcatcaacac caatttcttt taactgttgt gcacatattt tagccatatc aactctaact
    11461 tggtcactct cacctgctgt tatctcgaaa gctaacttag taccttcttt ttcatagatt
    11521 ccatcagaac ccagtttcca acctagcttt tcaatttctt gtttagcttt ttctggatta
    11581 tattcaaact tttctatatc aggattatta tatggtccca tttgtaaagg tgaatatgct
    11641 ggtacaccat gtcctaataa tacactgtca actattgctt ttctatctat cgcatagctt
    11701 aaagcatttg gcaatccttt agctttttta tctttaaaga atttagaatt aaaattgtat
    11761 aatatacctc tataatctgc tgttttcatt atatttactt taaaattctt ttcgtctttt
    11821 tcaaagttag acatatcttt aggagttact tgggctaaat ctaattctcc tgattttaat
    11881 tgcattgcct tagccttatc atctggaaca atcttaaaga ctactttatc taatcctggt
    11941 tcttttacaa aataatcact attcttaaca agtgttatac tttgaccttt gtcccatttt
    12001 tcaagtttaa atggtccagt acctattggt ttttgattaa attcatcagt agctatatcc
    12061 ttaccttcca aagcatgttt tggtaatatt cctactgtta aataatcaag cattgctgta
    12121 tttggagctt ttaaagtaat ttttatagta ttatcattaa ccacatcaat ttttgttata
    12181 tcttcatagt ttgatgctat ctcagaagca ttatctggat tcattatagt ttctattgta
    12241 aacttaacat catttgctgt aaatttctct ccatcatgcc acttaacatc atctcttaaa
    12301 ttaaaagtat atgtatttgt tgcctcatca aatttccagt cctttgctag acaaggaacc
    12361 accttattat tttcatcatg agctgttaat ccattaaata ttaatgaatt tatttctcca
    12421 tgttcatata gtgcaggatt tatacttgta tagtcattac ttccataaac aagcaccttt
    12481 ccatctttag gcgtatctgc cttcttatct ttatctcctc cactagaaca acctgccatt
    12541 agactaagta tcattactaa acttaaaact tttaacttct ttaatttcat gatgctcccc
    12601 cttttgcttt ttataaaata tttttcaaag ctttgacccc atttatttaa atagatattt
    12661 caaatacctc agtatttgct tatttagtac tcaaatgcta atttataaat tactagattt
    12721 tttattatta ttctttctta tataattacc tatattcgtt atacacacca atgtggtaac
    12781 caaaaatata cccggaatca gtataatcca ccatctattt gaaagtaatg cttcctctga
    12841 caaagataac atacttcccc aagaaacaat ttctattggt aaaccaattc ctaaaaagct
    12901 aagtgtcgat tcagtaccaa tagctgcgcc tatgtttgtt acaaccatga acattattga
    12961 tgccacaaaa tttggaagta aatgttgctt taatacatag aagaatcctc ctcccatgct
    13021 cttagctgct agtatgtatt cgctatttct tatttgtcta acttctgttc tcactatctt
    13081 agatatattc atccaacttg ttatccctat tactatggac atagatactg gatttgaatt
    13141 tccaagtata gcttgtacaa atattataat taatattgaa ggtatactaa gtatgatttc
    13201 tgtaaacctc atcattgcat catctacaag ttcagatgct gaaccactta tactaccata
    13261 aacaattcct ataacagttg atattacagt ggaaaatagt cctataaata aagatatttt
    13321 tcctccatac catatcatag aatatatatc tctacccatt gagtctgttc caaataaaaa
    13381 agttttattt ggagctaaat tagaacttgc taaatccatg tatgttggtt catgtgtcat
    13441 tataaaagat gagaatacac ttccaatgac tatgattgat aaaataataa ttgatatata
    13501 tggtaattct ttaatttttt tagataaggt tttctttgtt tctatatgtt catcttctgc
    13561 tactaaaata taattttctc ctacaatttc aaaatcacta tattgtggct tcattagtat
    13621 cacctctatc atatttcatt ctagggtcta ttttattgtt tataatttga gctaacatat
    13681 tcccaaatac tactaaagcc cctgttatta agcataaaac taataacata ttataatcat
    13741 gatactttgc actttcaaaa caaagtgttc ctagcccagg ataagaaaaa actttttcaa
    13801 ctacataagt tcctcctagt atatgaggta tcgaaatggc cattatacta atatatgatg
    13861 gcattatatt tctcaggcaa tgcttaaaaa caatagttct attatttaaa ccttttgctt
    13921 tacataaaag cacataatct tctcttattt cctctagtaa tttatttctt atcatatatg
    13981 tgtaatacca caaatggctt aatataagta ctgtaagagg taagattaga tgtgatatcc
    14041 tacttaatat actggactct tctcccattg aataagcacc actacttgga agtatgctta
    14101 agtttatact aaatataagt atgagtacta aggcaaccca aaatgatgga atacaattgg
    14161 ttattgttcc taatttgcat atgattctat caatgagctt atcttcatgt aatgtacaaa
    14221 atattcctaa caataaggct aacacaaagg taagtatata tcctgaaccg cctaatatta
    14281 tagtgtttat atagacatca tttataacag aagtaacatt ttgtttatat ttaaatgaaa
    14341 tcccaaattc cccttttgat gcatttgaaa tccattttat atattgactg tatattggtt
    14401 catccaaacc aagttttttc atagcatttt ctttttcttg tgtactcatt ctttctactc
    14461 catccccata gtaagagatt aatgggtctc ctggagcaag tcttgccatg taaaaaacaa
    14521 taaaagataa taaaaacatt actaaaataa actgcaacaa ttttttcagt atatccttta
    14581 acaaacccca accccccttt gttgcaataa aaaaaaccac ataaaagcaa acgtaaagta
    14641 acgttaaaac cttcgtggtt ccattatcat taaacaaaat taaattttca atatttatct
    14701 atgagttaat tttagcatca tttattaact gtgtaaacaa actttttaaa aattgttaaa
    14761 atttcatcaa attattttat ttatacctaa aataaaattt ttatattcta tccttcatta
    14821 ttttctataa aattataaaa ttttaggttg actatataag aaaagtatat tatattagtt
    14881 tgtatgcaaa ccatttaaga ggtgatttta ttgaaaggta attatgactg ttttagaata
    14941 gccatgctct taaaagaact ttattctaaa actatgtaca cagtagaaga aaattttaaa
    15001 gaaaatggat taacacacca gcagattata gttataaaac tgatagctca taatcaagag
    15061 cttacaatat cacaactatg tgatgaaatg tctttagcta aaggtacggt ttcaggcata
    15121 ataagcagat tagaacagat tggatatata gaaaaattca agaaatctaa tgataaaaga
    15181 aatacatacg ttaagtttac aacaacagga tttgaatttg ccacaaattt taaaattaaa
    15241 atgcaagaaa gttttgatga tatttttaaa aattgtgatg aaaacgaatt gagtgactta
    15301 gtaaaaaatc ttagaaatat attggcaaaa gtaaaggaga gataattatg aattataaat
    15361 taatacttgc aatagtattt ataacatcag ctttaatttt ctacactata ggtgtatttg
    15421 gagaaagaaa ggccaaaatt ttaaaaaaga aacatgtaat tatattttgg cttggtttta
    15481 tatttgacac tttaggaaca ttcacaatga gtaacatagc caacagccat acttttgaag
    15541 taaaatctgc tttatcacaa aaccttcata gtattacagg tcttcttgca attgtgctta
    15601 tgctttttca tgcaagttgg gctacttttg tcctatacaa agatgatgaa gagaagaaaa
    15661 aattctttca taagtttagt atagttgtat ggactatatg gttagttcca tactttatag
    15721 gtatgtttat tggtatggct ggatagtaaa ataaaaagtg tgtaagaaat actgtaggta
    15781 aatttgtata agacaaattt atctacatcc ttctttcaca cttttacata tttatctata
    15841 atactccatt atttctttca tatttcttaa tacctatata gaatatttaa ttgtttctat
    15901 agtattttat tattttctca cattttttca tatgtatata tgaaatattt cattatttct
    15961 ataatgtttt attatctttc tcatactttt tcatatatat attgcttcac tatttttttt
    16021 atacttcttt attaaatact tatgtatacc atacatttaa taaactctaa ctattaaaca
    16081 cctaatactt gagttactcc tggaacttca ctcaccaaaa cattttcaat aatagctttg
    16141 attgtcatag ttgctcctgg acacccacta catgcccctt gtagtctaac taaaactaca
    16201 ccattttcat ttacatctat aagctctaca tctccaccat ctctttgtaa tactggtttt
    16261 atcttttcct caagcacttt ttcaactttt tctctcatag ctattccccc cagaaatatt
    16321 ttattaccaa aattatacca atggtttaaa tttttgtcca tctaatgatt tatcaaattc
    16381 aatttttgct tcatcaataa ttttagtatc cataaataca tcataagcac tacaagccag
    16441 tactttagct gaatttatca tagcagaaaa tcctatattt gaacctgctg atgaacatga
    16501 ctgccatgta tgacttgcaa ttccaacagg ccacgctgcc attgcaaatt gagccactgg
    16561 tattacataa cttacatctc caacatcagt agaaccagct agtcctttat ctttgtcacc
    16621 gtcaactatt tccttatgta tatagctttc tacaatagat ttatcatttt gtaatgatgt
    16681 tgctacacct aatctttctt ctctagtaag agtatcacac aacttttttg caaattcttc
    16741 ttcttcagga gtatttttag ggcaaccaac aaaaaccata ttgtcataaa ctaaatcagt
    16801 caaacattgg tttggtatat agtcatatat tccatctgta accttatact ccatttcagt
    16861 ttctgtcatc attgctgctc cttgagctac ttttattaat ctatcaagaa catgttctgc
    16921 atcctttgct tttttacctc taataaagta ccaactttca gcaaatccag gaactacatt
    16981 aggtcttcca ccaccattag ttataacata gtgcattctt atagagtcaa taacatgttc
    17041 tctcaaataa ttagctccaa cattcataat ttcaacagca tcaagtgcac ttctaccgtt
    17101 atgaggagct tgagctgcat gggctgttat tcctttaaat ttaaatttta cagataagtt
    17161 tgccagactt ccacctctcc aaggtgcatt tatatcaaaa ggatgccaag taaatgcaca
    17221 atctacatca tcaaaacagc catttataca cataactgtc tttcctccac cttcttcttc
    17281 agcaggacaa ccaaaatatt ttattgttcc attaaattta ttttcttcta ttaatttttt
    17341 tatagaaaca actgctccta cacctgctac ccccaacagg ttatgcccac atccatgtcc
    17401 agcttttcct tctaccaatg gctttctttc tgcactcaca cattgtgata gctctggtaa
    17461 agcatcatat tctccaagta tgccaatcac aggtttaccc tctccaaaac ttgctataaa
    17521 tccagtaggt aaattttcta cttcttcaat tttaaatcct tttgacttta ggtattcttt
    17581 ttggatgctg cttgcatact tttcttcaaa ggctaattct ggattttccc atattttctt
    17641 agataaatct attaattctt ctcttatttc ttcaacatta tttatgatat actctttcat
    17701 aatcttcctc caaatatatg tatttttgat atgctttaca aattatatta tatactaaca
    17761 ttctatttat tcacaatttt ttgttaatta attttattca tatgttcttc tatggagtat
    17821 atcttatctt cactttgctt cttcataaat ttaatgtact ctatcttcca taaagtagtt
    17881 tcagtttttt tatttaatat aaattctaat atattaattt ctctcattct aatatcttca
    17941 taaaatacta cactacccaa tattgccaat tctaattcac ttaatgatgc aaacaacatt
    18001 aaatactctt ctccaaataa acattcagat tcaagaatat cgataaagtc tttcttagca
    18061 ttgatatttt cctttattat tcttattttt tcaattatag atgtactatc taatatctca
    18121 ttgaatatct ctctaaacga ttcatcatcc aatttacttt catcatcaac aatataatct
    18181 tctaagtgtt cctttttatt tttactcaca gttaccatac ttttaaaagt gttacttttt
    18241 attgagctta gcacagattc aataaatatc ttttcatatt ttttaatata tcttattaca
    18301 tcttcatctt ctatgcttaa tctttttatt aatacttcaa ttgcttccat aagtagtaaa
    18361 gttatttttt tatttattct aatttctctg ttaatttctt caaaatcatt ataatccaaa
    18421 ttactatcta cttcagtgtt aaaactatca tctacttcaa tcaattcaat tatattactt
    18481 tcttcagagc ctataacttt atcttcagta atattattgt ataaattttt aaattgtttt
    18541 tctaaaaatt catactcata ttttgaaatt tctaaattag tcacttcatt tcctaaaata
    18601 gtagaaaaaa tacaattatt tataataagt tcaaataaat ttacaagtat aacatcacaa
    18661 tctattttat aagtcactcc atagtcataa aataattttt ctctatcttt tttcttaaat
    18721 aagttacaaa atctattttc tatatctata ctctcaatat aattcttcac ataataaata
    18781 cccttaacat ccatatcatc tagtgccaat ggataatcta tacttactgg aacgtcctga
    18841 gcttcaaatt ttataatata acttttaaag aaacttgata actccatcaa ggtatcatta
    18901 tatgctattg aattagtatt caattttcta ctcaccatta gttcataaaa atcttttagt
    18961 ctaactaatt cttcctctaa tatttcaata cctccaaggt atactttttt aacattctta
    19021 gcagtcaact ccacaactct tttttctata tctccaagct tatttatata tgcatcaata
    19081 gcataccata ttgaaatcaa cagtttttcg gcaacttctg tttttactga tgtgctttgg
    19141 ccatttgtat attttttaat caaatccata agtattagag atatttcttg ttgtatatta
    19201 tatacttctc tttccgctat cactttacaa tttagacatt ccttaagtag agatatggaa
    19261 tattggttct ttattaaatt attttcgttt atattaatat tacttaaatt atttaacata
    19321 caaaatctct cctgtcatat attaaacttc tccaaattta aatttctttg caaattcgtc
    19381 tgcttctcct cttacattgt ccatattacc tgaaaacttt tcataaaatt cacccagtat
    19441 ttctataatc tcatcatcac atatcttgtc atcagtttca ttttttaaat aataaaaaat
    19501 ttcttgcata tcattaatag tctctaaata attgtccaca tttgtatatt gtgatgtata
    19561 tatattttct attaattgtt ttgtaacttt tatatctaat tctattcttc catacccctt
    19621 taaagtatta tctctcgaat ttattatatc ttttacatca gattcagaaa gagtcagacc
    19681 ataatctcca gattctatat tagtctcgag cagttcattt ctttctattt cctttaaatt
    19741 ccgatttata gaactcaaaa taataggtga tatcattttt aaattctcct tttttatttt
    19801 ttctattgac tttttttgtt ttttatgcta tacttaaatt aatatacatt attccactaa
    19861 ccacttataa tagtagtgtt aaaagtagtt tttgtcaact caaaattact atttcaatta
    19921 aacttttcaa ttttctcaac aaaaaatacc atcatttaga tatataattt tctctaacaa
    19981 cttttaattc agtggttatt tactaaaatc taaacaatga tatatttatt aattttattt
    20041 atcttttttg gctctacttc tacttttctt tacatttgaa caatttctac aagaaggtga
    20101 acagtattga gatttagaat ttgtaggaat ataaatttta ccacagtact tgcatttatt
    20161 taaagaagaa ggttcacttg taattgcaaa cgcatagata gtctcaatag ctgttttcaa
    20221 agaatcaaac tcccatgcaa taattggttt atctaattgg cttatagaaa atcctatctt
    20281 ttgaggatta aaccctccag acataatagt aacactttca gttaatagat tagatgtatt
    20341 tttatataca caaatttgat taaagtgctt atacatatct ttagcaaagc ttacaatcca
    20401 atctagttgt tcagtgtaga actttgaaaa tactaaatct aaaatcaatg gtctctttcc
    20461 aaaaaacttt ggagattcct ctctctttga tacatctata cctcttctat actttttaaa
    20521 atacacatct tcatcacttg caaatggtat aaacatgttt acatattcat ccgtactcaa
    20581 tatattctct tttgtaatat aattcttttc aatcatcagt actctttctt ctccaactat
    20641 atctctatta tatacacttg aatatattaa acccaataat ccatactttt ttacaaattc
    20701 aagtacttct ttctttagtt tcatttcttt tgtctctttt gatatatctt gtaatgcctc
    20761 ttgtcctatg cgaattatat ctactattat atcatctgct acatcaaatg ggttgtacat
    20821 ggtaaacaca gcatcttctg ttggaactat atataattca tcttcttcag taattttata
    20881 ttcataatca ctatacctaa tccaatcact tgcaaaatta cctattaatt ctattttttt
    20941 attcatgata atcattcacc cctttcaatg taatatataa ttttatatta ctattacatt
    21001 tttagtttat tccatatttt ctatactg
//
LOCUS       DAEMAE010000050        20939 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEMAE010000050 DAEMAE010000000
VERSION     DAEMAE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.051"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(155..997)
                     /locus_tag="KPH93_001472"
     CDS             complement(155..997)
                     /locus_tag="KPH93_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1688300.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1258..3300)
                     /locus_tag="KPH93_001473"
     CDS             complement(1258..3300)
                     /locus_tag="KPH93_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG1688301.1"
                     /translation="MSILVTLLGLLVCTTTSTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEDAGIQFIITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFIIRFIWVYLWH
                     NSFIKTKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEAQLVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDENNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3452..4423)
                     /locus_tag="KPH93_001474"
     CDS             complement(3452..4423)
                     /locus_tag="KPH93_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG1688302.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4424..5401)
                     /locus_tag="KPH93_001475"
     CDS             complement(4424..5401)
                     /locus_tag="KPH93_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688303.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5620..7179
                     /locus_tag="KPH93_001476"
     CDS             5620..7179
                     /locus_tag="KPH93_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1688304.1"
                     /translation="MKFKKLASLILVSALMLTFTACASNDKDKSSGGKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGPFKFKEWKKGESIVFEKNTD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGIKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7285..8244
                     /locus_tag="KPH93_001477"
     CDS             7285..8244
                     /locus_tag="KPH93_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688305.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     AQDIENIRVNMGLDKPVYIRYLNWLKNTLNGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSIFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIEDLVKHLVLPVITLGLYNTAIFTNYVEAGVNEQLKKQYVVTAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8247..9059
                     /locus_tag="KPH93_001478"
     CDS             8247..9059
                     /locus_tag="KPH93_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688306.1"
                     /translation="MFTSILKELKKDKSFAFACITLSIIIILIMASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFYSNT"
     gene            complement(9276..10178)
                     /locus_tag="KPH93_001479"
     CDS             complement(9276..10178)
                     /locus_tag="KPH93_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1688307.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEENE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10533..11711
                     /locus_tag="KPH93_001480"
     CDS             10533..11711
                     /locus_tag="KPH93_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG1688308.1"
                     /translation="MKEVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPVPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11720..12376
                     /locus_tag="KPH93_001481"
     CDS             11720..12376
                     /locus_tag="KPH93_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG1688309.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLIEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12376..13041
                     /locus_tag="KPH93_001482"
     CDS             12376..13041
                     /locus_tag="KPH93_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG1688310.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDDVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGNI
                     SVV"
     gene            13146..13922
                     /locus_tag="KPH93_001483"
     CDS             13146..13922
                     /locus_tag="KPH93_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG1688311.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     IAELQKEGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13984..15318
                     /locus_tag="KPH93_001484"
     CDS             13984..15318
                     /locus_tag="KPH93_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1688312.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIKKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSVFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15815..16741
                     /locus_tag="KPH93_001485"
     CDS             15815..16741
                     /locus_tag="KPH93_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG1688313.1"
                     /translation="MMRKNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     NLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSDSTNKPDNNKPESNKPEDNNNSDSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNVERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(17135..20674)
                     /gene="nifJ"
                     /locus_tag="KPH93_001486"
     CDS             complement(17135..20674)
                     /gene="nifJ"
                     /locus_tag="KPH93_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG1688314.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7254 a         3277 c         2914 g         7494 t
ORIGIN      
        1 ctattataat ttttttataa acataagtct tgcctgcatt atcagtaaca atcttaatgt
       61 tttaaaatta ttattttata ttaaatttta aataatatat taaaattaaa cataatatat
      121 taaatctata cactctgtat taaactgctc tttatcataa attcagtaca gatataaccc
      181 ttttgatatg taatgttaaa taaagtagtt catcttgact tacatagtaa tcataattct
      241 tttctacatg agtttttatt ttatatgcac aatcataatc tttttcatat tttactttaa
      301 ttatatctat aatttcatta tttgtatcat tgattttttc tttttttact aatcgttttg
      361 caaagaattt taaatgagtc agaagcctat cataatttat atcatcttct ttaaattcaa
      421 ctctataata atatcttata atatttaata tgtcttttac aattttagta attaacaatg
      481 attcctttga acttccttta tagtttgaat ttactatatg cattgctata aaaccagctt
      541 catccactgg aaattttaca tcaaattctt tatttatata atctacagcc catttcccaa
      601 tctcatattc ttttttatgt attcttttta tctcatgtag taaatcattc tttatagtta
      661 tattttcatg atatctttta attgcaaatg caatatggtc agtaagtgct acataaatat
      721 attcataaag cttagtattc aaattctcct ctgcataagc aattatttca gtactaatct
      781 caaaaaattt ctcatcaacc tggcttgcta gtttactaat tttatctaaa aagctttcat
      841 cttgtattac aaaagttctt tcaattttac ttttatctat ttcttgacct ttcttttttt
      901 gaaacccaat tccacatcct gttaatatta attctttttt tatattttca tctaaaacta
      961 atacaacatt attattataa atttgttgaa taatcataat caatatctct cccaaaatac
     1021 aatttccctt attcataaaa aaatacctaa attaagatac aattcacctg tataccttaa
     1081 cctaggtatt gcctgcttac cagtaacaat ccagcttgtt aacgcttata tattgtattc
     1141 aatttatggt tttattctat tataaataaa tatatatgtc aagtaaatta ttgaaaacta
     1201 tttcctattt tttagtatta attttaatat caaaaaacaa atcttattcc attatattta
     1261 tgattctatc atcatcattt catcataaag aatattttcc aataactctt tagccacttc
     1321 tcttgatatt tgcttagttt caactaattg ttgaacactg tctctttgac tttgtatcgc
     1381 cattactctg tagtattcta ctcttgcctc aaacttatca tcattttgaa tctttctagt
     1441 aatccaattc aaataacgat tatagaatct gattgctatt aaggcctcat gtctgttatt
     1501 ttcatcagta tttttattta taaatgatat agcagtttta gtagaaagtg catatgcatg
     1561 gtgaaattct ttgattattt ctttattctc agatactatt tttgtaattt tttcttttac
     1621 ttctttcttt acttttctat ttataatcca catgcttatt tttaatttta caattttaaa
     1681 tatagatttt tgaacagatt ttcttgattt tgtaatataa aatctataca atttaaatgc
     1741 tctatcagaa attttaccat cttctaatag tttcaaaaca gctaataatc cctgttggtc
     1801 cgcttctgca atcatcatac ttataagttt gttatctgga gtatttagta ttcctttttc
     1861 tgagtctacc aactgttcat ttaaatcatg tataactagt tgtgcttctt gtgctttttc
     1921 attactaagt tttaaaatgg cttctttcaa aattagttta tatgcctctt tatcagataa
     1981 taaaggttct gactcttgta cgttattttt taataatctt ggcagtaaaa ctgttgccaa
     2041 tactaagctt ataagaataa cttctgatga tataaataaa acagtgtctc tcattggaaa
     2101 tacatcttta tcagccaaat aaaatggtat agataaggca gttgctagtg taaatgtacc
     2161 atgaacacca caagtagcaa ctattagtga gtatttacac ttagaaatac tctcttttac
     2221 aacttcttca tccttaaaac ctaaaaaacc tacaaaaaaa ttatttaaag gattcttttt
     2281 agtttttata aaagaattat gccataaata tacccatata aatcttataa taaataaaat
     2341 aagtacaatt aaagtagaaa taaaagtcaa ttccaatgcc atattttcat ttttactact
     2401 aagtcctgta tatatacttg gtaacaaaaa accaagcagt gtaaagacaa atccattcaa
     2461 tacatactcc aaaacatacc aagtattgtc tgatattatc cttaatttag tagttgtatt
     2521 ttgtaaatgc tctttttcaa ttccatgagc tatccctgcc attacaacag ctaggattcc
     2581 agatactcct atttcttctg ctacaaaata tacaaaaagt ggagtcatta tttgtataac
     2641 tattaccatt ggaatttctt ccaaattcca cttgtgtaaa ctaagtctta tttttataat
     2701 tatgtatcct aaaatacttc ctactattat tcctccaaaa gctgtaatta taaattgaat
     2761 ccctgcatct tctattgaaa aaactcctgt aactgtggca agtactgcaa ccttaaatgc
     2821 tactactcca gcagcatcat ttaataaaga ttcaccttca agtatactca tcatattttt
     2881 aggaaaattt aatccttgtg taattgattt tactgctact aaatcagttg gagatattac
     2941 tgctgccaaa gcaaatgaaa cagataatgg catcccagga ataagaaaat gtataaagat
     3001 tccacctgca aacacggtta ttaaaactaa accaaatgca agaagcaaaa ttggttttct
     3061 aagctctaat agctcttttc tcgatacatt ttgtccttca ctgaatagta atggagctat
     3121 gatacatatt acaaatattt ctggattaaa tgaaaatgag aatttaaatg gcaaaataga
     3181 caatataatc cctagtacaa tttgatatat tgcaagagga atattactcc actttttgct
     3241 aaatacagtt gaagtcgttg tacaaaccaa taatcccaac aatgttacta aaattgacat
     3301 aactacctct cttttcaata ataatattat agattaaact tatatatcaa ctaacttaat
     3361 attaacagca tgaaatccca tcttaatcaa aaaaattttc agtttattct attattctta
     3421 ttatatacat caaatttaat ttttgaaata tctatactag atgacatgca acaaaatgat
     3481 ttcttgatat ttcttttaaa actggaatct cttttttgca tatttcctta caataagggc
     3541 atcttgtatg aaacttacat ccacttggtg gatttgttgg agatggtatg tctcctttta
     3601 atataattct atttttctta attgtagggt cttgtattgg tactgcgctc ataagtgctt
     3661 ttgtatacgg atgttgagaa tgtaagtaaa gttctccttt agttgcaatt tctattatag
     3721 aacctagata cataacacaa actctatgtg caatatgatt tactacactc aaatcatgag
     3781 atataaataa atatgaaagt ttatatttat cctgtaaatc cataagtaaa tttataattt
     3841 gagattgaat agaaacatct agtgcagaaa caggttcatc acaaactata aattctggat
     3901 ttaatgctaa tgctcttgct atacctattc tctgtctttg acctccagaa aactcacctg
     3961 gttttctatt taaatggtac ttagaaagac cacaactttc tacaacattt aatatattat
     4021 cttttatttc attctttggc actaaattat gttctataac tgcctcggat attatttgtt
     4081 ccacatttaa tcttggattt aatgaactat atgggtcttg aaatatcatt tgcattttgg
     4141 gtcttaactt tctaagttct tccttctcta acttatatat atctacacca ttaaattcaa
     4201 ctttaccttc agttttttca attaatctaa gaatggttct tcctgcagtt gattttccac
     4261 atcccgattc tccaactata gccaaagttt ctcctttttt taaatcaaaa gaaattccat
     4321 ctactgcctt tacacattta atttccttat caaataatcc agatttaact tcaaagtact
     4381 ttttaagacc ttctactttt aataaactct cttctttttt caattatatc tcctcctttt
     4441 tatataacca acatgcaata ctatgaccac tattcaattc tattaaagga ggtatctgag
     4501 ttctacattt atcacatact ttttcacatc tatcactaaa ataacatgaa tctggtattc
     4561 caattggatt tggtacttgt cctggaatcg aatacaatct tttatctttg ttttttccaa
     4621 gaacaggctt tgattttaga agtccaatag tataaggatg ttttggattt ttaaataatt
     4681 caacaacctc tgccacttct ataattttac cagcatacat cactaatact ttgtcacaca
     4741 tttctgcaac tactcccaaa tcatgtgtga tcatcattat agatgtgttt aatttttctt
     4801 ttatattttt cattatatct aaaatctgag cttgaatagt aacatctaaa gctgttgttg
     4861 gttcatcagc tataagtaat tttggattac atgataaagc catagctatc attattcgtt
     4921 gtctcatacc acctgataat tcatgtggat atgaattata tatttcttca gctcttggta
     4981 tttctaccag ttttatcata tcgattacct ttttcttagc ttcttccttt gataaatctt
     5041 gatgtatcat aataccttca atgatttgac ttccaatagt aaatgctggg tttaaagatg
     5101 tcattggttc ttgaaaaatc attgatatct cattccctct tattcccata agttcatctt
     5161 cattcatctt taaaatgtct ttgcctcgaa agattatttc tccaccttct atttttgatg
     5221 ttggaagtaa cttcatcaaa gacatggctg ttacagattt accacaccct gattctccaa
     5281 ctattccaat agtttcacct tcatttatat taaaacttac ttcatttaca ctctttacaa
     5341 tccctttatc tgtatgaaaa taaactttta aatctttaac ctcaattagt gctttactca
     5401 ttattttctc ctctcagcaa aattacttaa aataacatac accttttgat tgtattttat
     5461 aaaaatttaa attacaacat tttcttacat tttattattt ttttctatta attataataa
     5521 tataatagtt aatttctata tatattgatt taataatgat tactattata caatgaaaaa
     5581 tatgtgttaa atattatttt atggaggttt tataagaata tgaaatttaa aaaattggca
     5641 tcattaatct tagtttcagc attaatgctt acatttacag cctgtgcttc taatgataaa
     5701 gacaagtcta gtggaggcaa atctggtgga actgttgtaa tacctatggc aagtgaccca
     5761 gatataatca atggagcttt tgcaaatgtt aaagaagact ctcttgcatc aaatatagtt
     5821 tatgctcctc tttatactta tgaaaaaggg aatttagtaa attatttagc agaaaaagta
     5881 gattttaaag atagtaagga attaactata aaattaaaat ccaatttaaa atggcatgat
     5941 ggaaaaccaa taactgctga agatgtttta tttacattta atacagtttt agacgaaaaa
     6001 caaaattcac catcaagaca atacttattg gtgggtgaga aaccagttaa agtagaaaaa
     6061 attgatgatt taacagttaa gattacttta ccaactgcaa gtgaatcttt tttatatggt
     6121 atatctaaaa tttctccaat accaaaacat gtgtttgaag gagaatcaaa tatagctaag
     6181 tcagaaaaaa ataataatcc agttggttct ggtccattca agtttaaaga atggaaaaaa
     6241 ggtgaaagca tagtatttga aaagaataca gattattttg gtggagaacc taaggcagat
     6301 tctatagcac taaaaataat ccctaatgaa gcatctcaag aagcagcttt aaataatggt
     6361 gagatttcac ttatgaagac atctgctgaa ggatatgaaa aggcaaaatc aaactcaaac
     6421 ttacaaactt atacttatag tgaagaaaga ttaaactaca ttgtatttaa ccaaaatata
     6481 tcaaatatgg ctaacaaaga agtaagacaa gccttatcat atgccttaaa tagaaatgaa
     6541 atgatagaat cagcatatgg aaaagaaggt tctgtacctg ctaaatctat attagttcca
     6601 gaagcagact tctatacaga agaaggtgtt gaaggatatg accaagatac aaataaggct
     6661 aaagacttat tggataaaag tggtattaaa attgataaat taaaaatagg ttataacaca
     6721 ggtagatttg ggcataaaaa ctatgcatta gtagctcaac aagagttgaa aaaaattggt
     6781 atagaagctg aaatagttcc ttatgaaagt aaagcattct tcaatatact atttagtaat
     6841 agcactgaat gtgatatgta tgtaaatggt tatgcttggg gattagaacc aaacccatat
     6901 agaggtatgt ttgaaactgg tcaatattgc aaccaaacta aatattctaa tgctgaaata
     6961 gatgctttat gggaaaaagg atttactgag ttaaacaagg aaaaacgtga agaaatatat
     7021 aagcaaatac aacaggatat atctaaggat gcacctattt acactataga ttatgaacaa
     7081 aatttaatgg cagctcaaaa aaatctaaaa ggaataaaag atgctaaacc atctcctgca
     7141 atactatttg aagattggtc taaactgtat gtagaataat tgatataaaa ttttatatta
     7201 ttaattagaa aaggagatgt ctaaaaataa tattgtttta ttattttaaa atgagacatc
     7261 tcttttaatt aggagaattt atttatgtta aaattaatat taaatagagt aaaagtttta
     7321 atacctatgt taatcttaat atctatatta tcatttggac ttttagaatt agctcctggt
     7381 gacccagcag atgcatatat aaatccttta atgtctgcac aggatattga aaatataaga
     7441 gttaatatgg ggcttgataa accagtgtat ataagatatt taaactggtt gaaaaacact
     7501 ttaaatggaa acttaggcat ttcttatata aaccatatgc ccgtgacaga acagattatg
     7561 gaaaagatgg gaaatacatt tattcttatg ggtacttctt taatattttc aatattggta
     7621 gctatacctc ttggaatatt tttagctgta aataaaaatt caataactag taaagtatca
     7681 tctattttta attatatagg tgtttctatc ccttcttttt ggataggtat gatactcatt
     7741 tcaatttttt cagttaaact taacattttc ccatcaggag gtatgcatac tattggaaat
     7801 gatagtattg aagatttagt aaaacattta gttttgcctg taataacttt aggtctatat
     7861 aatactgcta tttttacaaa ttatgtagaa gctggtgtaa atgaacaatt aaaaaaacaa
     7921 tatgttgtta cagcaagagc aaaaggatta tcagaaaaag tcatactatt taaacatgtc
     7981 ttgaaaaatt cactgacatc attggttaca atcttaggca tgagtataca aaaattagtt
     8041 actggtgcat ttgttacaga agtagttttc tcttggccag gtatgggaag attaatgatt
     8101 gattcaattt tttcaagaga ttatactgta attatggcaa taactatgtt atcggcacta
     8161 tttttaattt taggtaattt agttgctgat atactatact tattaattga ccctaaaata
     8221 aaatctagca aaggaggatt ctagttgtgt ttacaagtat cttaaaagaa ttaaaaaaag
     8281 ataaatcctt tgcttttgca tgtattactc ttagtataat aatcatatta attatggctt
     8341 cttttatatt aaatatagac tctaatgcaa ttgatactac ttctaaactt caagaaccta
     8401 gcttaaaaca tatctttggt acagacgaac ttggtagaga ttacttctct agagctcttt
     8461 atggtggaag gatttcactt agtgttggta ttttatcaat gcttatttct gtagtatttg
     8521 gtactgccgt aggtgttatt agtggatata ttggtggtaa gctagaccaa ttcttgatga
     8581 gactagtaga tatgcttatg agtataccaa gtttcttagt tttgattgta ttaaatactt
     8641 atctaacacc taaagtttcc actattatac taattattgg attcttaagc tggatggaag
     8701 tttcaagaat tgtacgtggc gaaacattaa aaataaagga aaatgaatac tgtctagctg
     8761 ctaaagctac tggtatcaaa actagaaata tactattaag acatatagtt ttaaacttaa
     8821 aagagactat tgtagttgct ggttcattaa atattgctaa tgctatctta actgaatctg
     8881 ctcttagttt tttaggtctt ggaatccaat tgcctcttgc atcatggggt aatatgcttc
     8941 aagatgctca aagacatatt tttgacaagt tatatttagc tgtatttcca ggattactta
     9001 tatttttaac agtcatgagt ttgactataa taagcaaaaa attttatagt aatacttaat
     9061 aaattttata tagaaaataa aaacagtgac tatctaaaga catacactgt ttttattttt
     9121 cgttttaaaa aaatacatat tttgtatatt ataactttaa atactactct accatgttat
     9181 gtaaaaccta ctattgctct caagcacata gatgaaaagc taatagttta ttataataaa
     9241 aatggattta tttatttgtt actttaataa catttttaag agttcttttt gctaaagaaa
     9301 tctataataa atttaaatac atgttggtct acatggctat aacgttcttt tttaggtcta
     9361 catagtataa ctttccatgt aataggatga ttaaaaggct tttctataat tttgctattt
     9421 tcaaaaaaat catgaactgg tgatggtaga attgttataa aatcagaatt tgtggttgat
     9481 agcaacaaaa aatcccatga accagacatt atatatcttt ttaacttaac attttcatca
     9541 gtaaattttt gcattagctt atgatgtatc ataaaagtag gattaaatat tgcaagtaac
     9601 tgctcattca aatcgttcca atctatctta tccttcttag caagtggatg atttatattt
     9661 aaaaatgctg taagttcatc ttcttgaagc acatgttcag ttactacact tttatcaatt
     9721 ttatgtggat gtaacagtac tgcataatct aattcctgta agataagcat tctatttaag
     9781 tcatatgcac ccttctctac tatatcaaat tctatatctg gattatttgc aactaactgt
     9841 gcaactacat ctgaaaatgc tattccaagt ataagtggag gtatccctat tctaactttt
     9901 cctttaaatt gaactgaatc ttctcttaaa tcttccatca tatttttgta ttcatttata
     9961 atatgttctg catttacata aaatctttct cctgctggtg ttaacccatt taacctacct
    10021 ttataccttt caaataaata tacatcttca ttttcttcaa aattttttat aatttgactt
    10081 agtgctggtt gagatatatg aagtgtctta gatgctattg ataaattaaa accagatttc
    10141 actatttcaa caaaatattt caactgtttt atatccattt taaaccccct tccccaatag
    10201 tataatttag attatatttt ttcttataat gatattttat aggaaaatca acataatttc
    10261 attaatattt taacaaaata tttttctaaa tctactctaa atttttatcc tataaatttt
    10321 tcttatacac tatataaaaa attagactta gacaaaacgc ttttttcgcc gtaatctata
    10381 attagaaaca ggaaaaccta aagcattagg aataaaaata attgaaatac ttaaaaacaa
    10441 gcttgtatta aaaaagtact ttattatagt gttccctatt ttttttattc agaaaatttt
    10501 taattatcat tcaagaaagg taactattag atatgaaaga ggtagtaatc gtatcagcag
    10561 tcagaacacc tataggaagt tttggaggtg tatttaaaaa tacttcagct gttcaacttg
    10621 gaactatcgc tgttaaagaa gcgatatcac gtgtaggact taatttatct gaaatagatg
    10681 aagtaataat tggaaatgtt cttcaaacag ggcttggaca gaatgttgct agacaaattg
    10741 ctataaatgc aggtatacca aattctgttc caagctacac agtaaataaa ttatgtggtt
    10801 cagggcttaa aagtgttcag ctagctgccc aatcaatcac atctggtgaa aatgatgtgg
    10861 taattgcagg tggtactgaa aatatgagcc aagctcctta tatcgttcca actgctcgct
    10921 ttggtagtaa aatgggtgac ataaccatgg tagatagtat gctaacagat ggccttattg
    10981 atgctttcaa tcagtatcat atgggaataa ctgcagaaaa tattgcaact aaatttgaat
    11041 ttactagaga aatgcaagat aaattggcat tagaaagtca aaataaggct gagaatgcta
    11101 taaaaaacaa ccgctttaaa gaagaaatcg ttccagtaga tgttttaata cgtcgaggaa
    11161 aaatagaaac tattgataaa gatgaatatc ctaaattagg tatgacattt gaaggtttag
    11221 ctaaacttaa acctgcattc aaaaaagatg gcactgtaac agctggaaat gcctcaggta
    11281 ttaatgatgg tgcggctatg ttaatactaa tgagccaaca aaaagcagat gaactaggta
    11341 ttagaccact tgctaaaata aaatcctatg cttcagctgg tgttgaacct gaagtcatgg
    11401 gtactggtcc agtccctgca actagaaaag cactgaaaaa agctgggctt agcatcaatg
    11461 atatagactt aatagaagca aacgaagcat ttgccgccca agcattagct gttaaaaatg
    11521 aacttcaaat agattcatca aagttaaatg ttaatggtgg tgcaatagca ttaggacatc
    11581 caataggagc aagtggagct agaatacttg ttaccttaat ttatgaaatg caaaaacgta
    11641 aagttgaaac tggacttgca acactttgta ttggtggtgg tcaaggaata tcaatggtgg
    11701 tttcacgata ggaggaaata tgaataaaat agttagcatt gatgaagctc tatctcatgt
    11761 aaaagatgga gatacaataa tggttggtgg attcatggcc aatggttcac cagaaaattt
    11821 aattgattat ttatgtagta aaaatataaa agatttgaca ctaatttgca atgatactgg
    11881 ctttattgac aaaggagttg gcaaaatggt tgtaaataag caatttaaaa agataattgc
    11941 ttctcatgta ggattaaata aagaaactgg aagacaaatg aacaacaatg aaacagatgt
    12001 tgagttagtt cctcagggaa ctcttgctga acaaatacgt gcagctggtt atggtctagg
    12061 aggtatactc acaccgactg gtattggtac tcttattgag gagggaaagc aaaaaattgt
    12121 tgtcgatagc aaagaatacc ttcttgaaaa accaatttat gcagatgttg ctcttctatt
    12181 tgcatcaaag gtcgataaag caggtaatct agtttataaa ggctctatga ataattttaa
    12241 taatttaatg gcatctgctg ccaaaattac aatagttgaa gcagatgaaa tcgttgaaat
    12301 tggcgatatt gacccaaatg aagttaatac accaggtatt tttgtgaatt acatcgttga
    12361 aggaggtaat ttataatgga taaattagaa atgcaagaat atattgcaaa tagagtatct
    12421 aaagaattaa aagacggagc cgttgtaaac cttggaatag gtctaccaac caaagtagca
    12481 aattatatcc cggatgatgt aaacgttatc ctacaatctg aaaatggctt tttaggttta
    12541 ggttcagctg aagatggaaa ttcaagtgat gaaactattg taaatgcagg tggtcaacca
    12601 gttactatac ttcctggagg atgtttcttt gatagtgcta cttcttttgg aataatcaga
    12661 ggaggtcatg ttgatataac tgttcttggc gcacttcagg ttgacaaata tggcaatata
    12721 gcaaactata tgattccagg taagatggtt ccaggtatgg gtggagctat ggacctagta
    12781 actggagcta aaaaagttat tgtagcaatg gaacatactt caaaaggttc tgcaaaaata
    12841 ttaaataatt gcactcttcc acttactgca acaaatgctg tagatttgat agttactgaa
    12901 atgggagtta tggaagtaac atcagatgga attttactta aagaaataaa tccagctttc
    12961 acattagatg atgttatttc tgctactgaa gctcctttaa tattgtcaga ttcattaaac
    13021 ggaaacatat ctgttgttta aaataattaa aactagaaaa taaaactctc ttagacgtat
    13081 tacatataaa tttgtattgc ttaaaatatt aatataaaaa taaaataaaa ttggaggaaa
    13141 tgaaaatggt taaagataaa gttgtattcg taacaggagc tgcaagtgga ataggtaaac
    13201 aaatagctga atcgttctta aaaaatggtg ctaaagtaat gttctctgat ataaatcaag
    13261 aagctttaaa taataccata gcagagttac aaaaagaagg ttatgattgc atgagtgtaa
    13321 agtgtgatgt aactaaggaa gaagaaatta accacgctat agacaaaact gttgaaaagt
    13381 atggcagatt agatatatta ataaacaatg ctggacttca acatgtttct atgatagaag
    13441 atttcccaac tgataaattt gaatttatga taaaaataat gttaacagca ccttttatag
    13501 ctactaaaaa agcatttcct ataatgaaaa aacaaggctt tggtcgtata ataaatatgg
    13561 cctcaatcaa tggtgtaata ggatttgctg gaaaggctgc ttataactca gcaaaacatg
    13621 gacttattgg actaacaaaa gttgctgctc ttgaagctgc cgattctggt ataacagtta
    13681 atgctttatg ccctggatat gtagatactc cacttgtcag aggtcagttt aatgaccttt
    13741 caaaaactcg taatatacca ttggaaaatg tcttagagga agttttatac ccacttgtac
    13801 cacaaaaacg cctattagat gttcaagaaa ttgccgacta tgctatgttc ctagcaagtg
    13861 ataatgctaa gggtattaca ggacaagctt gcttactaga tggaggatat actgcacaat
    13921 agttattata tttagggctt gaagaaagta ttcttcaagt ccttctacta aggagggata
    13981 tttatgagta ttatcgcacc aattggtata attattggta ttatagctat tatatatttt
    14041 tcagttaaag aaattcatat aactatagct gctccaatag caactttaat agttgttctc
    14101 ttaaataaaa tggatgttgt aacatcaatg ctaggtgctg acaaaaacaa ttatatgggt
    14161 gcacttggaa actatataat gagttatttt gcaatattct tattaggctc aatacttgct
    14221 aaatttatgg aagaaagtgg agctactgta tcaatagctg attttatttt aaataaattt
    14281 ggctctaatc atccttacag agttttagtt gctatattta tagtaagttt tatattaacg
    14341 tatggtggta taagtttatt tgtagttatg tttgcagtca ttccacttgc aagaactcta
    14401 tttaaaaaac ttgatatttc ttggaacctt attcagatac cactttggct tggtattgca
    14461 acaattacta tgactataat tcctggaaca ccagctattc aaaatgttat acctattcaa
    14521 tatctaaata cgtctcttac tgcagctact ataccaagta ttgtaggtag tataggttgt
    14581 gctacgtttg gacttgtcta catgaaatat gctctaaaca aaagtattaa aaaaggtgaa
    14641 acttatgcaa cttacacttc agaattagaa gaagaatctt tagatagaga attaccagga
    14701 ttttttgcaa gtatttttcc tctattagca ctcgtaatta tagctataac tggaagtact
    14761 tttggttcag aatttatgaa aaaaaatgta atttacatag ctttaatagt tgcaattctt
    14821 ttatctgttt ttttattcca taaatttcta tcatctaaga tacagacatt gagcataggt
    14881 gctagtggtt ctataggtcc tatatttgct acatcttctg cagttgcatt tggagcagta
    14941 ataatggctg cacctggttt tgatgtattt agcaaactta taatgtcaat accaggtagt
    15001 ccacttataa gtcttactgt tttgacttca acaatgtcag caataacagg ctcctcttct
    15061 ggtgcattag gaatagtaat gccaaacttt gcagattatt atttaagtac tggcctgaat
    15121 ccagaattaa ttcaccgtgt tgctacaata gcctcaaata tactaactat agttccacaa
    15181 agtggtgttt ttcttacatt cttaagctta tctaagttaa cacataaaac tggatttaaa
    15241 gaaactttca ttgtagttgc agttggttca ctaatagcag aaattattgt aattacatta
    15301 ggattactaa tgtattaata taaaacaaat taaaattcat gatatgaaag gttgtgtctc
    15361 aaagtgaact ttttacttaa tgagacacac ttttttgtat gaaataatat atattttaat
    15421 catttcaaca ataaaaaaga acctttctct attttgagac agaccctttt tattccttaa
    15481 agatttaaat aattataatt tgttactaat acgaaagcat ctattgaata aatttaacta
    15541 aataaaaaaa aatttatcat aactataaat ttacgttata gaattattat aaaaatatta
    15601 ttttctctaa gtaagtaatt ccatactata cagcttttaa cacaaattat caaacacact
    15661 taaatatacc tatttattcc atatatatct tttttacata tctttacagt aaaaaaagtc
    15721 ttaatcttat gttagtatag attcacgggt accccctaca ctaaaggtta caattcagta
    15781 acatttttag aatttttaga ttaagaggtg tttaatgatg agaaaaaata taccaacaaa
    15841 attaaaaact atattagctt taggagtgtt aaccttagca acagcttcta gtgctgttac
    15901 tgcaagtgct tcatcacttg aaaataatgt aaatataagt aacaaaaatt tagtatacag
    15961 taatactaaa tacaaatttt ctgcaaaacc aaattgttct gtaaaaccag aacttaagcc
    16021 tgataaagat aactcttgta aggataagaa tcataatgac aagaattgca acaacactaa
    16081 taaacctgag gacaacaata attcagactc tactaataaa cctgataaca ataaacctga
    16141 gtctaataaa cctgaggaca acaataattc agactctact aataagcctg ataacaataa
    16201 accagagtct aataaacctg aagacaacaa taattcagac tctactgata aacctgataa
    16261 caataaacca gagtctaata aacctgaaga caacaataat tcagactcta ctaacaaacc
    16321 tgatgataat aacaactcag gctctacaag tgaaaatttt tcagcttacc aaaaagaagt
    16381 tgtcgactta gtaaatgtag aaagagctaa agctggatta aatcctctta ctcttgattc
    16441 ttctatatca aatgttgcta caaaaaaatc tcaagatatg attgataaca actatttttc
    16501 acacaattct ccaacttatg gttctccatt tgacatgttg aaaaaatttg gaataagcta
    16561 taagacagca ggtgaaaaca tagctatggg tcaaaaaact cctaaagaag ttgtaaacgc
    16621 atggatgaat tctgaaggtc acagaaaaaa tataatgaat ccaaatttca gtaaaatagg
    16681 tgttggagta gcacaaaaaa gtggtggttc aatttattgg acacaaatct ttgtaggata
    16741 aaatactctt tttccataca tatcaaaaga gtatctctaa gtgataaaaa acacaaatga
    16801 gatactcttt tgtatctatt ttaaaattat caaatccaaa tatacatact ataaatatta
    16861 tttgttctct taattttagt atgtagaaca atatataaaa agtattggca agatgaaaat
    16921 ggaaaactta aaaaagatat ttaatagata aaaacaaaat cttaatatct tcataaaaaa
    16981 aggactgtct caaaatagag ataaggcctt tttcattttt gaaatgatta aaatctattt
    17041 gatttcatac aaaaagagtg cctcatgaac taaaaagctc accttgagac actctctttt
    17101 atcaattcta tataagcatt ataatatttt aatattattc agctaatttt ttgtatcctt
    17161 catatctttc tttagcatca gcttcagctt tagcaaataa tccatcagct tcctctggga
    17221 attgtttagc tatagcagaa tatcttactt gtgcaagtat gaaatctttg aagcttgcag
    17281 ttggttcttt tgaatctaaa gtgaatgggt ttttaccttc agcttttact tctggattga
    17341 atctgtataa atgccagtat ccacaagcaa cagcttgtgc ttcgttagct acagttcttc
    17401 ccataccttc ttttaatcca tggtttatac atggagcata agctattatc agagatggcc
    17461 catcatgttt ttcagcttct agaacggctt tgataaattg atttttatca gcacccatag
    17521 caacttgagc tacatatacg tttccatatg tcatagccat cattcctaaa tctttctttc
    17581 tagatttttt acctgaagca gcaaacttag ccatagcagc agctggagta gctttagaag
    17641 cttgacctcc tgtatttgaa tatacttctg tatcaaatac aagtacgttt acatcttcac
    17701 cagatgcaag aacatggtct aatccaccat aaccgatgtc atatgcccaa ccgtctccac
    17761 ctagtatcca ttgagatctc ttaactagat aatcttttct atcttttata gcttttacta
    17821 attctttagc tttttcatct gtacaatcac aagctggttt ttctaataat tcaacaacct
    17881 tagcacttgc tactttagaa gcttctccat catttctaga atctaaccac tctgtaaata
    17941 ctgctttagc atcttcacag atatccatag aaactaactc tgtcatagca tctactattt
    18001 tatttcttat ttgcttaaca gctgtgtaca taccaaatcc atattcagca ttatcctcga
    18061 ataaagagtt tgcccaagat ggacctttac cttcatggtt acaagtataa ggaattgatg
    18121 gtgcagaagc tccccatata gaagaacatc cagtagcatt agctatcatc attctatctc
    18181 catataattg tgtaactact ttaatgtatg cagtttctcc acatccagca catgctcctg
    18241 aaaactccat taatggttgt ctaaattgac ttcctttaac agtatttgca ggcataacat
    18301 caacttttgg agctacttca tcaggattta ctgcaaatgc ccaattatct aactctgtat
    18361 caagttgagt atctattggc ttcataacta aagctttttc tttagctgga catatgtcag
    18421 cacagtttcc acatcctgta cagtcaagtg gactaacttg aattctatat tttaatcctt
    18481 ctaatccttt gcctaatgct tttttagctt caaatccttc tggagcatta gctaattctt
    18541 cttcagtaac taatactggt cttatacatg catgaggaca tataaaagaa cattggttac
    18601 attgaataca gttatctatt atccattcag gaacatcaac agctatacca cgtttttcat
    18661 aagctgcagt accacaaggg aatgttccat cttctatacc attaaatgta cttactggta
    18721 agttgtttcc ttcttgtgca gtcattggtc ttagtatatt ttttataaat tctggttctt
    18781 taacttctcc tttatctgca tcaacagcat tttcccaaga agctggaact tcaactttaa
    18841 ctaaagcatt tattcctgca tcaacagcgg catagttcat attaactatt ttttcacctt
    18901 ttttaccata agctttaact atagaatctt ttaagtattt aacagcatct tcttctggta
    18961 ttatttcagc taatttaaag aatgcagatt gcattatcat gtttattctg tttccaagac
    19021 ctatttcttg tcctagttta acagcgttaa ctgtatagaa ttttatatca tttttagcta
    19081 tatattgttt catatgagct ggtaaatgtt cttcaagtcc tgcttggtcc catatagtgt
    19141 taagtacaaa tgtaccacct tttttaagac cttttaataa atcatattga taaacatatg
    19201 attgtttatg acaggcaata taatcagctt catctattaa ataagtagct cttattggag
    19261 tatcaccaaa tcttaagtga gacatagtta taccaccaga ttttttagag tcataatcaa
    19321 agtaagcttg agcgtatttt tcagtatggt caccgattat tttgatagct tgtttgttag
    19381 ctccaacagt accgtcagaa cctaatcccc agaacttaca tcttatagtt cctttagaag
    19441 ctatttttat tggttcagat ggagttaaag aagtattagt tacatcatca actataccta
    19501 ctgtaaatcc atttttaggt tgttctgaaa ctaagttatc aaaaacagtt tttatatctg
    19561 aaggagtagt atcttttgaa cctaatccgt atcttccacc tattatcata ggagcatttt
    19621 cttttccata gaatacatca cgtacatcta agtataatgg ttcaccagtt gaacctggct
    19681 ctttagttct atcaagtaca catattcttt caacagtaga tggcatagca tctaagaaat
    19741 gtttcattga gaatggtcta tataaatgaa ctttaactaa accatatttt tctcccttag
    19801 catttaagta atctatagtt tcttctattg tttctgttac tgaacccata gctattaata
    19861 cgtatttagc atcttctgct ccatagtaat caaataaact atgttttcta cctgttaaat
    19921 tactcatttt ttccatgtat ttttcaacta tacctactat attttgatag tatttattag
    19981 aagcttctct agtttggaaa tatatatctg ggttttgagc agtaccacga gcaactggat
    20041 gatttggaga taaagcatta tctctaaatg cttgaactgc ttcaaagttt aataaacttg
    20101 cataatcttc attttctagt aattcaactt tttggatttc atgtgaagtt ctgaatccat
    20161 caaagaaatg tataaatggt aatctacctt ctattgcagc taaatgtgca acaggtgcta
    20221 tatcagctac ttcttgaact gaaccagaag ctaataatac acatccagtt tgtctagcag
    20281 ccataacgtc ttggtgatcc ccaaatattg ataacgcttg agatgctaaa gcacgagcac
    20341 ttacatggaa tactcctggt aataactctc ctgcaacttt atacatgtta ggtatcatta
    20401 ataataaacc ttgagatgct gtaaaagtag aagttaaagc tcctccttgt aaagaaccgt
    20461 ggaatgttcc tgatgcacct gcttctgact gcatttcaac aacattaact gtttgtccaa
    20521 atatattttt tcttccttgt gatgcccact catcaactac ttcagccata gttgaagatg
    20581 gtgtgattgg atatatagct gctacatctg taaacgcata ggcaacatga gctgcagctg
    20641 tatttccatc aagtgttttc ataaacttag ccataatatg tatcctcctt gatagctaca
    20701 attgtacttg tttataacaa gtctattttt ttatatttac atataattta cacaacaaaa
    20761 atataaacac acatatagtc gcgcaaatac tctatcttct aatataatac tacagattag
    20821 ataaaattcc tttgaaaatt gtaaattaat tacatattta agtagatttt ttttaaaatt
    20881 aaatatgtta agtttttttc ataattttgt atacaatata taataaatta agttagact
//
LOCUS       DAEMAE010000051        20153 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEMAE010000051 DAEMAE010000000
VERSION     DAEMAE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.052"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(208..351)
                     /gene="scfA"
                     /locus_tag="KPH93_001487"
     CDS             complement(208..351)
                     /gene="scfA"
                     /locus_tag="KPH93_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG1688315.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(417..779)
                     /locus_tag="KPH93_001488"
     CDS             complement(417..779)
                     /locus_tag="KPH93_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG1688316.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(837..1130)
                     /gene="yajC"
                     /locus_tag="KPH93_001489"
     CDS             complement(837..1130)
                     /gene="yajC"
                     /locus_tag="KPH93_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG1688317.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1264..2385)
                     /gene="tgt"
                     /locus_tag="KPH93_001490"
     CDS             complement(1264..2385)
                     /gene="tgt"
                     /locus_tag="KPH93_001490"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG1688318.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2464..2949)
                     /locus_tag="KPH93_001491"
     CDS             complement(2464..2949)
                     /locus_tag="KPH93_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688319.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2961..3986)
                     /gene="queA"
                     /locus_tag="KPH93_001492"
     CDS             complement(2961..3986)
                     /gene="queA"
                     /locus_tag="KPH93_001492"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG1688320.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKANGNNVICVGTTSCRTVESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4304..5323)
                     /gene="ruvB"
                     /locus_tag="KPH93_001493"
     CDS             complement(4304..5323)
                     /gene="ruvB"
                     /locus_tag="KPH93_001493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG1688321.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5414..6016)
                     /gene="ruvA"
                     /locus_tag="KPH93_001494"
     CDS             complement(5414..6016)
                     /gene="ruvA"
                     /locus_tag="KPH93_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG1688322.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6009..6518)
                     /gene="ruvC"
                     /locus_tag="KPH93_001495"
     CDS             complement(6009..6518)
                     /gene="ruvC"
                     /locus_tag="KPH93_001495"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG1688323.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6831..7460)
                     /locus_tag="KPH93_001496"
     CDS             complement(6831..7460)
                     /locus_tag="KPH93_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688324.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7663..7986
                     /locus_tag="KPH93_001497"
     CDS             7663..7986
                     /locus_tag="KPH93_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG1688325.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8254..9108
                     /locus_tag="KPH93_001498"
     CDS             8254..9108
                     /locus_tag="KPH93_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688326.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     VTVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9294..10730)
                     /locus_tag="KPH93_001499"
     CDS             complement(9294..10730)
                     /locus_tag="KPH93_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG1688327.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10801..12183)
                     /locus_tag="KPH93_001500"
     CDS             complement(10801..12183)
                     /locus_tag="KPH93_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1688328.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSIMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKGSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12319..13224)
                     /gene="garR"
                     /locus_tag="KPH93_001501"
     CDS             complement(12319..13224)
                     /gene="garR"
                     /locus_tag="KPH93_001501"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG1688329.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13620..14291
                     /locus_tag="KPH93_001502"
     CDS             13620..14291
                     /locus_tag="KPH93_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1688330.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEP
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14393..14941
                     /locus_tag="KPH93_001503"
     CDS             14393..14941
                     /locus_tag="KPH93_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688331.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFDENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDSKVSEGKYTYGNKNCK"
     gene            15203..15511
                     /locus_tag="KPH93_001504"
     CDS             15203..15511
                     /locus_tag="KPH93_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688332.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15673..17463)
                     /locus_tag="KPH93_001505"
     CDS             complement(15673..17463)
                     /locus_tag="KPH93_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688333.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17463..19190)
                     /locus_tag="KPH93_001506"
     CDS             complement(17463..19190)
                     /locus_tag="KPH93_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688334.1"
                     /translation="MKLILSYLKNYKLLIVLNVLAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19435..19998)
                     /locus_tag="KPH93_001507"
     CDS             complement(19435..19998)
                     /locus_tag="KPH93_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBG1688335.1"
                     /translation="MLEVVNMRKVVLYIATSLDGYIAEENGSVSFLDEISSEFKNEQE
                     DDYNTFYDTIDTIIMGNSTYVQIANELSPNEWYYKGKECYVYSNTINGTTENVKYTNL
                     EPKKLIEQIGKEKEKKDIWIVGGGDIVKLFMKDNLIDDYYIYILPIILGRGISLFKSG
                     IDKTNLNFKKASNIGELVKLEYSKKQK"
BASE COUNT         6659 a         3500 c         2328 g         7666 t
ORIGIN      
        1 aataaaatat aagagcagat actttaaaat aaatgttctt atatgattag atattcttgc
       61 ataattaaac gataagaaat taaatttaat tttacataaa tagaatacat atttttatat
      121 aataattgaa aataaaagag gatacctcat aatgataaaa gaatcacttt gagataccct
      181 cttacaaaca actaaatcaa taattttcta tctttcacac tcttgatttg caactgtaca
      241 agaagtttta caagctgatt gacaagaagt ttgacactct ccacatccac cttttgcagc
      301 actttgcttt aaagtagctt gagataaagt ttttacatgt ttattttcca ttttcaacgc
      361 ctccatttct aattgtttcc tattgattat atcataaaac tactttaata taaactctat
      421 ttaaagttga cacccaatac gcctccaatg cctcctgctg ctgatattag caataactta
      481 taaatcatcc ctacttcaaa tacaaacttt tcttgtgcta aaaacacagt caaaaatata
      541 catactatgt acaaaagtcc tactaaaagc ccatacataa gtcctttttc tttcatgtgt
      601 tttgaagtgt aaaaccctcc tatagctgca gaaatagtag ttataaatga tgagaccata
      661 gttatattat ctccaccaat atctgtaaat gtcaaaaata agttatacac taataaaaca
      721 gccaaagtta ttatatatgc atatcctaga cctttaaaaa tataattaga ttttcccata
      781 aaaaaacctc ctctcaaatt aatatatatt cgagaaaagg cttttaaatt actaatttag
      841 tcttcttcta tttcttcttt tttagattta acagttccta tagcccattt ttcaaatgga
      901 actttagttc tatttggtcc taattctaat attacaacat catcttcaac tttagctaca
      961 ttagctacta ttccacctat tgtaatgacc ttgtctccta cacttaggct acttctcatt
     1021 tcttttaatt gtttgtcttt tttcttttga ggtctaatca ttaggaaata aaagattgcg
     1081 attactacta cccatagacc tatactcata attatttgtt gctgtggcat taatttttca
     1141 ctcctttgta aattatactc ttttgtaaat cataataaat gtttatataa caatttactt
     1201 tctctatctt tgagtactat tgaatattat atattaaaat gattataatt aatagtgcaa
     1261 aacctatatt tcatatccat attttgcaaa aaattcattt ctaaaatcaa gtaatctatc
     1321 ttccattatc gcttgtctta tttgtttcat taaatttaat aaaaagtgaa gattatgagt
     1381 agttattaat cttgcaccca gtatttcatt tgcttttata aggtgtctta tataagctct
     1441 tgaatagttt ttacaagcat aacaatcaca ttcagggtca agtggagtaa agtcttcagc
     1501 ataagttgca tttctaacta ctactttacc ttggctagtc attgcagttc catttctagc
     1561 gatacgagta ggtaggacac aatcaaacat gtctactcct cttataacac cttccactaa
     1621 gtcatctgga ctaccaactc ccattaggta tcttggttta tcctttggca taagtggagt
     1681 tgtatgctct agcacgtcat acataagtgg ttttggttct ccaacactaa gcccaccaat
     1741 agcatatcct ggtaaatcaa tactagttat ttcttttgct gattgttctc ttaagtcttt
     1801 atacatccca ccttgtatta taccaaataa agcttgtttg tcggtattat tatgggcatc
     1861 tttacatctt tttaaccatc ttgttgttct ttctaaagaa ttttttacat attctctatc
     1921 tgatgggtat ggtgcacact catcaaacgc catcattatg tctgaaccca atgcattttg
     1981 tatttccata gctttttctg gagttagaaa atgttttgaa ccatctaagt gtgacctaaa
     2041 ttctacacct tcttctttta tctttcttaa aggccctaag ctaaatactt ggaatcctcc
     2101 actatctgta agtattggtt tatcccaatt cataaactta tgaagaccac cagctctttt
     2161 tataagttca tgacctggtc tcatatataa atggtatgtg ttactaagta ttatttgtga
     2221 accaatctct tttaattctt ctggtgtcat agattttaca gttgcttgag ttccaactgg
     2281 catgaatatt ggtgtttcaa taatcccatg tggagtgtgt aatcttccta accttgcacc
     2341 actctgctta caagtcttta tcaattcgta tcttactgcg tacataaagt tctccttaat
     2401 catatttatt tctcattatt tcttaagata tatttgtaat aagctattca tataatttat
     2461 tgtctaaata gatttatctt cgtcttcttt gttctgttgt tccttaattg cttcttcatc
     2521 atgaatactc attatattaa gagcactctt gtcttttgtt actaaaaact tttttatagc
     2581 aaatagtcct gctatagcta taactcctat taaaacatct atcatacctt catttttagg
     2641 cagtagcaac atttttctag ctattgcgta aagtaatgcc tcaataaatg ctcctggtat
     2701 atgaagagat agcattacta caagttcaac acctattact aaaagtaata tttgtgctaa
     2761 aaaatcatta agctgactgt attgtactgg attttgaaaa tcaactatat atgctcccca
     2821 cataagtctt agaacatcta tagttcctaa aaatactgct attagaacta ctactgctaa
     2881 aacacgttca aatataaaag caatctttaa tatagggtta ttcttttttt gtttttgtaa
     2941 actatgcatc cattacctcc ctactttata atcatagcat ctccaaaact gaagaatcta
     3001 tatctttctt ttactgcctc attatatgca tttaaaacat tatcctttcc acaaatagca
     3061 cttactaaca ttattagtgt agactctggt aagtgaaaat tagtaataag tttatctaaa
     3121 actttaaatt tataacctgg atatataaat atttctgtcc atccactagt ttctttcatc
     3181 tttccatcct cattacaagc tgattctaca gttctacaac tagtagtacc aacacatatt
     3241 acattgtttc catttgcctt cgctctattt attttgtcag ctgcttcttg gctaaccata
     3301 taatattctg aatgcatctt gtgatttaaa acatcttcta cttttactgg tctaaaagtg
     3361 cctaaaccaa catgtagagt tacaaaagct atatctacac ctttttcttt tattttattt
     3421 aacaattctt ctgtaaaatg taatcctgct gtaggtgctg ctgctgaacc attatgcttt
     3481 gaataaacag tctggtatct ttctttttca tctagtctag cagtgatata tggaggtagt
     3541 ggcatatttc caagttcatc caagatttct tcaaaaattc catcataatg gaattttata
     3601 attcttgaac cttcatctga taaatctaca acttctccaa ttagctttcc atttccaaaa
     3661 gaaaatttag tccctacttt tgctcttttt cctggtttta ctagagcctg ccaagtatct
     3721 tcctctgttc tttttaataa taaaaactct attttacctc cagtttcaag cttctctcct
     3781 attaatctag ctggtataac tctagtatta tttaatacta agcaatctcc tggatttaaa
     3841 tattctatta tatctctaaa tactttatgt tcaatattac ctgtctcttt atctaatacc
     3901 atcaatcttg agctagctct atcttctata ggaacttgag ctataagttc ctgtggtaag
     3961 tcaaatttaa aatcacttgt tttcaatgtt aaacttcctc tcttaaaatt tctccagcta
     4021 caataatatc aaaaaaacaa cttattttcc acaatattac cataatctat caagaaattg
     4081 ctatttttaa cagacaccat atccacaata tatagatatt tttccacgat atacatgtct
     4141 tatccacaat atatagatac aatacacaca atttttatcg tgtttatcca cagtatctac
     4201 agatataatt accattttgt ggatatgttt gaatttttag ccattttaga aatattttaa
     4261 tttaatgact cttttatttt ataaatactc gtataatcta tttttatttt tcattaggat
     4321 atggaatttt taagtgttca tatgctaatg gcatggctac tcttcctcta ggacctctat
     4381 ttataaatcc taattgaagc aaatatggct catatacatc ttctattgta tttctatctt
     4441 ctccgataga agctgccaat gtatctaaac ccactggtcc tcctctaaat ttttctatta
     4501 tagtcataag aagcttctca tctacaaaat cgagacccaa actatcgact cctaaaagtt
     4561 ccagagcatc ttttgccact ttatcagtta tttttccatc agctctgact tgtgcaaaat
     4621 ctctaactct ttttagtaat ctatttgcta tccttggagt ccctcttgaa cgttttgcaa
     4681 gttctaatgc tccattagac tgtatctctg catctaagat gcttgaagac ctaagtacta
     4741 tttttgaaag ttcatcaact gtataatagt ccaatttaca aataacacca aatctatctc
     4801 taagtggatt tgtaagcata ccagctcttg tagtagctcc aataagtgta aatttaggaa
     4861 ggtcaagtct tatgcttcta gcagatggcc ctttccctat tattatatct aggcaaaaat
     4921 cctccattgc tggatataaa acttcttcaa cacttctatt gattctatga atctcatcta
     4981 taaataaaac atcattttca tttaaattcg taagtatggc tgccaaatct ccagctcttt
     5041 ctattgcggg tcctgacgta attcttaaat ttacccccat ctcattggct attatacttg
     5101 ctagtgtagt ttttccaaga cctggtggtc catataaaag cacatggtct aactgctcat
     5161 ttctcgattt agctgcttct ataaatatac taagttgttc tttggatttt tcttgaccca
     5221 aataatcttc taaagttttt ggtcttaaac tgttttctat atctatatct tccatcttca
     5281 ttgtagaagt tattatccta ttttcatctt caaaaccttg catttatacc cctcctctcg
     5341 tacttttgaa aatctacttt atatattttc aaatttacat aaactcctta aatattgttt
     5401 taaaatctaa atattatttc aaggatttac tcattatata tgtaagtgct ttctttataa
     5461 tagcttctgt attcatacca tcttttttac atttttgaac tgcctctttt gattccgaca
     5521 aagtatagcc taaagctaca agtgcatcta cagactcatc ttgtgatatc aaggtaggtt
     5581 tttgtgataa taacgttggt tcaaactcta tgttattttt gtctacctta tccttaagct
     5641 ctaagacgat tctttctgct gtctttttac caacaccagg agctttggat aattttccaa
     5701 tgtcttcact taaaatatat gctcctaact gtgctggtga tgcaaatgat agtattgaaa
     5761 gcgccacctt aggtcctatt ttagatacag atgttagtaa ttcaaacatc ttaagttctt
     5821 ccctacttac aaaaccacaa agactcatat catcttctct tacaattaat ttagtatata
     5881 tttttgtggc ttctcccact tgtaaattca ttattgtatt ggatgataca tttattttat
     5941 atcctatccc attattttca acaactatac tgtctatata aatttcttct actgtacctt
     6001 ttatataact atacatactt accacctata ttctttagag ttttttcaag tttatttgaa
     6061 tgagcatggc aaatagccac agctaaagca tctgcaacat catctggttt tggaactttt
     6121 tttagactta aaaatgaagt taccatctgt tgaacttgtg ccttatctgc tctcccatat
     6181 ccaactacac cttgttttac ttgaagtgga gtgtattcat atacaggctt cccattatga
     6241 gcacatgcaa gcatagtaac acctctagct tgtgcaactg ttatagctgt ttttacattc
     6301 ttgttaaaga ataattcttc cattccaact tcatctatat tgtaattctt tactattgta
     6361 tcaattcctt tatatacaag ttccaatctt ctcgatatat tcatatgggc aggtgttgta
     6421 actgctccat aatcgattac cttaaacttg ctatttttgt attcaattat accatatcca
     6481 actatagcta tacctgggtc aatccccaat attatcattt tttaaaattc cccctaaaaa
     6541 taaaagaaat accaaaatgt atttctcata aaaacttatg ttctaaatca ttatatcata
     6601 aataatatta acaaatcata aaatgctcta tgtaaaatat aaaatcaaat aaaaaagtta
     6661 tatcaaagca agatttttac ctcaatataa cttttatatt tagtacaaat aaattattgc
     6721 atttttatag gaattaaatg tgactacaat tttaatacta atctatttaa tattaaatta
     6781 tttattatta acttacttag tatttaactt gaaatagtat taatttacta ttaacttgca
     6841 aagtcttcta atcttgaata ggcctcttct tcagtatcca taactacacc ttttttgatt
     6901 tcatcttgaa ctgttttagg aagtgaatct actcttattt ttgttttttc gactaatgtt
     6961 ttacttccac tatcatcata tttatatagt gctatatttc cattatcatt ttcaatagta
     7021 tatttacctt ttatagtaac atccattgct tgtacatctt tattatcact atatttagat
     7081 tctaccaata tgatttcatt tttatccata ctctttattt ttttagttgg atattctttt
     7141 ttaaaatatg atactatttc tgacttcgtc ttatctaata atgactctgg aactattcct
     7201 gccattgtaa gtgttctctt atagttggtt tcattttcgc cttcactttg aagccaaact
     7261 tcacaatttt tatttaatcc aaaagcttct tttgactttt cattcatatt cgcactagta
     7321 cttacaactt caccagtttg aactccattg tgttttaatc ttgtccctat ataaactcca
     7381 ctacttaata caaatactcc aactagtatt aaggcagata ttttccaagg aaataattct
     7441 tttttcttct tttcaaacat tgattacgcc tcctttttct attattttct ccaagctgta
     7501 agtttttata caatttaggt aaattttaga agaaaatata tttatatttc aaattactct
     7561 aagaatagat attttctaaa aggtagtttt ctcttaatag ttatattctt gtaattatat
     7621 ggacataatt tataataaat aatcgtttag gaggtaataa ctttggaaag aataaactgt
     7681 aatgtaatta actgttcaca taacaatagc catatatgtt atgcagataa aatttttata
     7741 aatggtgaaa aagctcacaa tgacagacat acttgttgta gttcattttt agatgaatct
     7801 atttatagta atttatctag caacgttaat aatagaaatt gtgaggaatt agtttgtact
     7861 gtaaaaaatt gtaaatacaa caaagatggt agcttatgta cacttgataa tatattggtt
     7921 gctcctgaaa ctgatagagt aaacctttat attgaaacat gttgctctag tttcgaatgt
     7981 aaatagtcat agtataaata taagggtgtc aaatgtaatt aaactaacat agtgtagagt
     8041 atctacaact attagtttga ttatacttga cacccttatt tattttaaat aaaatttcat
     8101 tattataacc atgtttataa tgttctaact gtaattaaat ttgataaatt tctataaatt
     8161 ggtataatca atatgtaaat tgaatttagc aaattaatca taaaaataaa atatcgagta
     8221 tatagtgtta tactaactag aaatgaggtg cttatgagta cattttatat tcaatttctt
     8281 tattttgtaa tttatagctt tataggctgg tgttgtgaaa ctacatattg ttcagtttta
     8341 caaaaacaat ttgtaaatag aggtttttta aatggtccat tttgccctat atatggattt
     8401 ggagcattag caatagttgc taccttgact ccttttgtac acaatattcc gttgttattt
     8461 ttgtttagta taattataac tagcataatg gagtattgta cgagctttat tttagaaaaa
     8521 atatttaata tgacttggtg ggattattct aaacataaat tcaatattca cggaagagtt
     8581 tgtctggaaa actcacttat gtttggtgta ttatcattga ttgtaatgct tatagtacat
     8641 cctattgtgg ttgattttat taactctatc tctaaaaatg tgctatttat atttgctata
     8701 tctatagaaa tttattttgt tttagattta gtagttacag tccatacaat tttacaatta
     8761 aatggtaagt taaaacaaat caatcttata attaaggaat taaaagataa gaaagaatac
     8821 tacaaactta ttacacaaga aactatagaa aacaaacttg aatctttagt tgaaaataga
     8881 ctggatactt tggaagatag gtttgaaata cttaaggaaa aagaaaacta tcgctatgct
     8941 aaaaatcgta ttgatgagct aaaaaataaa cttaatactt tgctgtctaa ccacaaatta
     9001 cttcatagac gtataatcaa agcattcccc aacataagct ctaataagca tacagatatt
     9061 ttaaataaaa tcaaagaaaa tattaaatca attaaaaaag ataagtaata aaataaacac
     9121 ataagttaaa taataaaaat actaatacat aagattaagg tttagaatat tttttgtaaa
     9181 atactctaaa ccctaattaa tatactatta tttttaatct taatttataa ttttagttta
     9241 ttttaatttt aactcagtaa atttaagcat ttaaacttca atacactaat atactacccc
     9301 atcaatatct caactgattc tctaagtgta ctaatctctc ccacatttcc agggaaaatt
     9361 atatatggca tattaggaaa tttgctttct ttgcctgtca tccatactgg tataccaggt
     9421 tttatctgtc ccattactgt agcttttttt actcttagtg ctttagtacc aacatcacta
     9481 gaagtaattc cacccttagc tataataaaa tttggtctta cattaagcat accaattata
     9541 ctggtaactg catctgatat ttctacagaa atcataagct gtttatcttt atcattcgta
     9601 tccaagtcaa aacgctctcg tctagtataa acagcaacag ttttaccact gcttattttt
     9661 tcctctacaa cttttataac tctttttacc tcatttttta gaccattttc ttgaagaact
     9721 aaatgttgat taaattctat aaattctata ggatatttac aatttttaag ttcttctaac
     9781 tgttgggtag tcttattcac atgtgaacca acaagtataa ttcctccatt agtattacct
     9841 tttgatacta actcatcctt agtaagcagt tccttatcac tcactccgcc aagtactttt
     9901 gttatggcag cagcgcttct aaacataaat tccttaccac tatttactgc tcgtataaac
     9961 gcaattgtaa atacctttac atctacataa tcggttgcat ttacaataat tttattgaat
    10021 ccttcagctt ttttaagttt ttctgttata gcttcaatat ttagctctct aagttctttt
    10081 gacgaaatat atatcatatc actagatttg tactttccat ttgtcttttc ttcacaccac
    10141 tcacctatat cagatgattt atatccaaaa gatttatctt ttgcaaattc agtcattcct
    10201 gctggtacaa gtatttctcc ttctttaaca taatgtacat tatctatagt aaacctacca
    10261 ccttctttaa aaaatggcat tattatttca ccctgaaata acttccccga taatctctct
    10321 acttcgcttt ttaataaatt agtttcaaca ggataatgtc ctctcatagt agaatcacta
    10381 cgacttatga ttataaaatc cttattaact ttctttgata catccacaat attctttgaa
    10441 atttctttat gtgcttttgt agtttgagca acagtaaatc cccttgaatt agtaagtata
    10501 aaaaacatac tatttttctc gttaaaacct tgttccatac tatccttatc ccaatcagta
    10561 taaacagaaa tatcatgaac tgtttgtaca cctgttgggt catcatctag tacaacaatt
    10621 tttctatcta aatgcttaag ctcatctgca agaagttcat ttaatctgtc ctcatctact
    10681 tgtttaaaag tattaagtat atctatacta attggtttta cttctctcat ttaaaacacc
    10741 cctttatgta atttgtatag tctaaatgta atttatatga tacaaacaat agtttgttat
    10801 ttatagacct ggaagtggta taattccatt tataacacta agcaataaaa ccattataaa
    10861 tgctacaact ccagcaatgg ttcctccaat tgtccaagag tttaaagtat ctttaactga
    10921 taatccacta aatcttgaaa gtacccagaa tccagaatca tttggaaggg agaaaccaac
    10981 tcctccagca catatagcaa gccctactaa aactggtgac attccagttg ccaaaattgt
    11041 aggtcctaaa attgatgatg ttgttactaa ggctacagta gcagaacctt gagaaattct
    11101 aagcaaggca cttaataaga atccaagcac tacaactgat atactaagac cagacatagt
    11161 atcaactaag aagttaccta taccacttgc atttataaca ctgccaaacg caccacctga
    11221 acctgttatc aataatatta gccctgctga atcagcagct tcaataacta catctcccat
    11281 aggtttattt atgtatttct ttaaaaatcc tattgccacc aatattccaa gaagtattgc
    11341 tatgttttta tctcctataa atgcaagtac tccactcatt gtagaacctt tttctaagaa
    11401 catactaaat acatttgata taagtataag aagtattggg aacaataaag aaaacataga
    11461 aattccaaaa cctggtactt tagaagaatc atcatcttca atttttatgc taccttttcc
    11521 ctgttctaaa tttttttctc tatcattctc atcaatagca taagctggat atttttcaaa
    11581 acgttttcca taaatccatc cacctataag agctgctgga aaagcaacaa ttatagaata
    11641 aagtataaaa ctacctacat ttgcatttac atttgctgca actgccaaag gtcctggtgt
    11701 aggtattaca agtgcatgac ctactatagt cccaactcct aaggcacata caaaaaccat
    11761 tatagatttc ttagttactc tcgaaacata cttaattata ggcatcatta taacgaaagc
    11821 tgcatccata aaaactggaa ttgaaattaa aaaacctgct gtagttactg ctaaagcact
    11881 gtttttttct ccagtcttag ctaacaaccc ctttgcaata gattctgttg ctcttgattc
    11941 tgaaagaata tttccaaata ttattccaag tccaattaca atacctatac tccctaatgt
    12001 atttccaaat ccagtcgata ttgtggttga gatttcattt ataggcattc taaccataaa
    12061 gcctgtaatt atagatgtta tcagtaatgc caaaaacgga tttactttaa atttaataat
    12121 cgatacaagt aaaattccta tagatataac aaaagctatc aaaagtccat atcctgtaat
    12181 cataattttt acccctttat cattctactt tattatttat cagtcttatt tttcttttac
    12241 aatgataact taacctcgct gtatttatat aatctaatcc taataatatg tatttaatta
    12301 tattgatata tttgaatatt aattattgtg tttttttatt tcaacacctg ccaacttttc
    12361 aaaatactgt gctattccac tatggtcatc ttctaaatgt ccatgtactt ttaatgtctg
    12421 cataatttct aataattgac ttgtcatagg tagtgggaca tctaaattat gtgctgtttg
    12481 cattacattc ttaatatcct tcaaattaat agatattttt ccacctggtt taaaatcacg
    12541 attcataatc attggtatct tcgcatctaa tattgtacta ccagctaggc ctcctcttat
    12601 tgccttatat actttttctg ggtctgcacc tgcttttgct gcaagtacaa atgcctcaga
    12661 aactgctgct atcgtcaaat taactattac ttggtttgta agttttgtaa cacttccact
    12721 accattatct ccaattaaaa tagatgagct tcccattata ttaaaaaatg gctctacttt
    12781 cttataaatt tcttccttac caccagccat aaaagcaagt gtaccatcaa tcgcttttgg
    12841 ctcccctcca cttactggtg agtctataaa ttctaagccc atatctttta atttatttgc
    12901 acacaatatt gattctgtag gagtaactga actcatatcg actactatac ttccttcttt
    12961 taaagttttt gccaatccat ccatcccaaa taatatatcc tgcacaatag ttccatttgg
    13021 aaggattgta aatacaatat cacattcttg acccatttct aaaacacttg cgtatttggc
    13081 tccacatgat ataagttcat ccacagcaga tttattaatg tcatacacca aaagattaca
    13141 tccatctttt aataaatttt ttgccattgg ctttcccatt attcctaatc caataaatcc
    13201 caatttcatt ttataaccct ccatatcttc acatttttga aaacgttttt tatgttttag
    13261 agtatactac ataccagtat accggtcaat ataaaatata aaagtattta ttttaacaat
    13321 aaatagtaat ttttaaagtc atttattata tttagtcatc ctatataagt tttaatacat
    13381 actattgttt tcaaatataa tatactactt ttacagtttt gtattttaac taatttcaat
    13441 tagttttaat taatctgcct ttaatgttaa tcatgcttca cacttcaata attttttata
    13501 ttttttaatc ttatctgcaa tttaaattgc aaaagattga ccttattttc actatgttgt
    13561 atgcttaaaa ttatataagt ttttgtattt ttatattgtt taggggggga ttattaagta
    13621 tggctacatt taaaagatta caagaacaag catatgacta cttaaaagaa ttgatactat
    13681 ctggaaaaat gatagaaaat gaaatatatt ctgaaacaaa acttgctagt gaaataggta
    13741 tctctagaac acctataaga gatgctcttc aaagacttag tcaagatgga tttatagata
    13801 taatcccaag caaaggattt agaatccatc aaattacagc aaatgaaatt gtagagatat
    13861 tccaaatacg ctctgcaata gaaggatttt gtactttttt aattacaagt caatacaaag
    13921 aacctagagc agtagaaact ataagtaaat taaaacatct tttagataaa caaaaagata
    13981 ttttattagg tgataaaaat ttaaactcat ttgcagaata cgatacatta tttcacacaa
    14041 ctatagtttc atatgctcaa aatactgaat ttgacaaaat gtttaacaat tatatgtaca
    14101 gaataaaaaa attagctctc gattcattgt cacatgaagg aaggcttgaa acaacattaa
    14161 aagagcacac agatattttt aataatattg caaatggatg tattgaagat atttacagaa
    14221 ctactctcat tcatatggaa actcctaaat acattaattt agaagatttt tgtaacaata
    14281 agttatttta gtattatact ttcatttatt aaaaatatat ctatctcaaa ttaatttcct
    14341 aatttataaa aaatattcta taattatatt gtaactaata ctattctaag gagtgatttt
    14401 tttgaacgaa gatgttgtaa ctaatgaaac tatcgacaaa gattatgcta ttgaagaagt
    14461 tcgtatggca tgcaaacatt ttggtgactt atatttttat ttttccaaag tgttatttga
    14521 agagtttggt gaagataaaa ctagtgaaat attaagaaaa gttttatttg aaaggtctga
    14581 agaaagagct attgctatga gagaaagagc tcatgaaaat ggagatgaat taattgcaga
    14641 caatattatt agcacaactg atgttccatt tctaggatgg gttcctgaat ttgaagagtt
    14701 acattgccct tatggagctt cctggctttt aagatttgat gaaaatcctt ggtttaaaaa
    14761 attcgcttct ctttattgtg atgttacaga tactactgta gctgaagtat ttacaggaga
    14821 tacgtcccat aaaataacaa aaaatattct atggggagat aaaagttgtg aaagaatata
    14881 tttccatgat agcaaggtat ctgaaggtaa atatacttat ggaaataaaa actgtaaata
    14941 aataatttaa attttctata ttcatatact atattttgta ctttttcata taaatattat
    15001 aactagtctt ttattttact tttgtttaac ctaaagataa tttactttta atctatatga
    15061 attatcatat aaataatcag aaagttatct ttataaatta tagttttaga tttataattt
    15121 ataaaatatt tatgttcttt gtaaaatttt ttagaagctt ctaatgttat gctataaaac
    15181 tatcttgaga agggctgatt gaatgtttaa taaaatatat gaaaagaaag aatttatagt
    15241 ttttcaagtt aaaaaaggtt atgtagttta taatactaga aagagttttg aagaaggtca
    15301 tacacattta aagcattttg aagcagcaaa gacagctatt gatttagcaa taaataaaaa
    15361 gattcctaga agcaaagatg gatattatct gactagttta attcgcataa gtgatgatgg
    15421 ttattacatt gataagttaa gcgaattgtt atatgttaga gaacaaaaag gtaaaaaaga
    15481 aaagtactgt aattctggtt atcagatgta acaagactag ttgtgaatta taagtaccaa
    15541 tcataagtac ctttggtctt aaaatcaaag agtatcagta aatctataaa aaaacaactt
    15601 actgatactc tttttaatta tatgattaat attttaattt gatacaaaat tttatataaa
    15661 agtttatgtt atctattctg attctttgtc tgcaaattgg ctattataaa gcttctcata
    15721 aaatccaccc ttttccatta attcatcatg attcccctgc tctataatac ttccattatt
    15781 cataactaaa atcaagtcag cattacgaat tgtagaaagt ctatgtgcta taacaaaact
    15841 agttctacca ttcataagat ttctcattgc tttttgaagc ataagttcca acctagtatc
    15901 aacagaacta gtagcttcat caagtatcaa tattgaaggg tctgatataa aagctctagc
    15961 tatggttaaa agctgttttt ctcctaaaga tatattagat gcttcttcat ttaagaacat
    16021 attgtatcca tctggaagag tggttataaa gtgatgaaca ttggctactt ttgctgcttg
    16081 tataatttct tctttagtag ctccaaatct accatattct atattttcaa aaatggttcc
    16141 attaaataac catgtatctt gaagcaccat accaaacatt gaacgcaaat ctttacgttt
    16201 catatctctt atatctactc catcaatttt aatagcacct ttctttacat cataaaacct
    16261 catcaatagg tttattaatg ttgttttacc tgctccggta ggtccaacaa tagctaccat
    16321 ctgaccactt tttacttctg catttaaatc ttctattaaa gttgaatttt ctccgtatcc
    16381 aaaattaaca tgctcaaatg taacattccc ttttacattt tctatcttaa ctggattttc
    16441 aacgtcaggt atttcctctt cttcgtctaa aatttcaaat acacgttcta tagctgcaaa
    16501 agaagattgt attactgtgg ataactgagt catttgtgaa agtggctgat ttatctgcca
    16561 tatataacgc acaaatgctt gtaaatttcc tactgttatc cctccactta ctgcatatat
    16621 agaaccgaca actgccactg ctgcaattcc aaagtaagtt actaatgaaa ccataggatt
    16681 catcatactt gatataaatt gtgccttaaa tccattttca caaagttctt gatttacctt
    16741 tttaaattct tttatggaat cttcttcttt accatatagt tttatttcat taaatccagt
    16801 atacatttct tgtacttttc catttaattt tccaagagca ttttgttgca atgaaaatct
    16861 actttgagat ttttttacta caaatttaga tacaaaataa ctaattggaa ttataaaaag
    16921 agctataaga gccatatata tattaattgt aaacatcatt gaaagtgcca aaattatgac
    16981 taaaattgag tttacaattt gcataaaact ctgttgcaat gcatttgata ttgtatctac
    17041 atcatttgat atacgactta atacatcccc aaaactatta ctatcaaaat aactaatagg
    17101 caatcttctt attttatttt ctacttcatt tcttaaatct ctcattgtgt tttgaatagc
    17161 attcgtaagt agaaaacttg aagtatacgt aaaaattgca ttggcaaaat atatacatgc
    17221 taaaatcacc aatatttttg ttacataatc aaaatttacg gacgcccctg gtactccttt
    17281 tgcaatacta attacatctt tagtcaattg tgttattatc aatccttcag ttttaggtgc
    17341 aagtgcattc atagtagctg ctgaaattat acatataata gctataataa aggaaaattt
    17401 atatttcttt acaaatggaa gaagtctacc aattgttttc ttaaaacctt tagacttttt
    17461 cattattcta attcctcctt ttttaactga gatgttgcaa tttcatagta tatttcacag
    17521 ttttttaata attctttatg agttcccatt ccgacaactt gcccttcatt gagtactata
    17581 atcttatcag catcaataat agaactaatt ctctgggcaa caatcaatac aattgcatct
    17641 tttgtttcct tcttcaattt agcacgaagt attgcatcag ttttaaagtc aagtgcagaa
    17701 aaactatcat caaaaatgta tatttcaggt cttcttataa tagcccttgc aatagaaagc
    17761 ctttgttttt gacctcctga tacatttgct ccaccttcac taatcaattc atcaaacttt
    17821 ctaggttttt ttgatataaa ctcatatgct tgtgcaactt ttgcggaata ttctaattct
    17881 gactctcctg ccttatgttt cccaaatcta atattatttg ctatgctacc agtaaaaagc
    17941 agtgattttt gaggtatgaa tcctatcttt tttcttaaag cttttaaatc atattctctt
    18001 atatctactc catttatctt aatgctacct tctgttacat cgtaaaatct aggtatcaag
    18061 ttaattagag tacttttccc actaccagta cttcctataa aagcaactgt ttcacctgtt
    18121 tttgcagtaa atgaaatatc cttaagtaca gaagcttctc catctggata ggtaaaagtt
    18181 acattatcaa attctatgat acccttattt tcagtatcct taattccatt ttcaggattt
    18241 tttatcaatg gttcttcatt taaaagttct tttattctat ttgcagatac ctctgctctt
    18301 ggatacataa taaataccat tgaaaaaagc ataattgaaa acatggcatg gaaaagatat
    18361 tccaaaaatg caaccaactg tccaacttga agttctccaa tatttatctt ctcacttgaa
    18421 atccaaaata cagccaaaac agctatgttt aatagtaaaa agaatgctgg ttgagtaatt
    18481 gacataagct taaataattt cttagaaaca ttagcataat atgtatttgt tttttcaaat
    18541 cgctcggttt cataatcatc atttccaaaa gctctaatta ctcttattcc agttaaattt
    18601 tctctcgata taaggtttaa tttatctagt cctttttgtt gttttgaaga tattgcctga
    18661 gatacttttg ctattattgc tactccaata attataaaag gtactgatat agccaatact
    18721 atagaaagtt ccacacttgt tctaattgtc attattatac ttatgataaa cataactgga
    18781 gttaataagg cagtccttag tagtatatta acaaattgtt gaatttgaaa tgcatcattc
    18841 gttgttcttg taatcattga agaaattcca aatttattgt attcagtatg tgaaaattct
    18901 tgtgatttct caaatatatc atttcttata tctctggtta cacttgttga taccttagca
    18961 gaacaataac ccagcaatat actacctaca acccctataa tagaaataat aacaatcact
    19021 attcccattt gttttatgta agctatattc tgatttgcaa taccattgtc aattatttta
    19081 gctattatag ttggaatccc caactcgact aatgcaaaac taaatatagc cagtacgttt
    19141 aatactatta ataacttata attctttaaa taacttaaaa ttaatttcat atttttctcc
    19201 tttcttaatt tttgagatag tgtaatcatg taataatact aaatttataa atgcttaatt
    19261 ttataaaaaa aggagaaaag agttaacgca ctctcttctc cagcatattc tttttaatac
    19321 accctccgac aagtcgctta tatgttaaca tatttgacaa gtaatgtcaa ttcatatagc
    19381 caaaagtctt ttaaatgctc catttcatga cattgtaaca aattggcatg taatttattt
    19441 ttgtttttta ctgtattcca attttacaag ttctccaata ttactagctt tcttaaaatt
    19501 caaattagtc ttatctatac cagacttaaa tagtgaaatc cctctcccca atattattgg
    19561 caatatatat atataataat catcaattaa gttatctttc ataaataatt ttactatatc
    19621 gcctcctcca actatccaaa tatctttttt ctccttctct tttccaattt gctcaattaa
    19681 ttttttaggt tctaaattag tatactttac attctcagtt gtcccattaa ttgtatttga
    19741 gtatacatag cattccttac ccttatagta ccattcattt gggcttaatt catttgcaat
    19801 ctgtacatat gtggaatttc ccataatgat agtatcaatt gtatcgtaaa aagtattata
    19861 atcatcttcc tgctcattct taaattctga agatatctcg tccaaaaaac ttacactgcc
    19921 attctcctca gctatataac catctaaact tgtggcaata taaagtacta cttttctcat
    19981 gtttactacc tccaacattt atagtgtgat tattctatgc aaacatcact tttttactca
    20041 ttcaataata ccctattaat aaatcctcaa actaatttaa ataaactttg cataaaatta
    20101 aaaatatatt atataaataa tttaaaacca caataactta ttgaaaattc taa
//
LOCUS       DAEMAE010000052        20083 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEMAE010000052 DAEMAE010000000
VERSION     DAEMAE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.053"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..1116
                     /locus_tag="KPH93_001508"
     CDS             46..1116
                     /locus_tag="KPH93_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688336.1"
                     /translation="MADIKLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLDVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGNPCMNILDIKNDVKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLDSEIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDLKICKNMYSKIKGDIYEKAI"
     gene            1100..1969
                     /locus_tag="KPH93_001509"
     CDS             1100..1969
                     /locus_tag="KPH93_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG1688337.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     VWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2013..2873
                     /locus_tag="KPH93_001510"
     CDS             2013..2873
                     /locus_tag="KPH93_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG1688338.1"
                     /translation="MDIIQPIKKDKVDIKKIVSKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKVLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            2904..4208
                     /locus_tag="KPH93_001511"
     CDS             2904..4208
                     /locus_tag="KPH93_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1688339.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4271..5533
                     /locus_tag="KPH93_001512"
     CDS             4271..5533
                     /locus_tag="KPH93_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG1688340.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYEDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGMVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFII"
     gene            5720..7690
                     /gene="uvrB"
                     /locus_tag="KPH93_001513"
     CDS             5720..7690
                     /gene="uvrB"
                     /locus_tag="KPH93_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG1688341.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKYYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIEDELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            7709..10534
                     /gene="uvrA"
                     /locus_tag="KPH93_001514"
     CDS             7709..10534
                     /gene="uvrA"
                     /locus_tag="KPH93_001514"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1688342.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIVLNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAKFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENDNSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            10796..12613
                     /gene="uvrC"
                     /locus_tag="KPH93_001515"
     CDS             10796..12613
                     /gene="uvrC"
                     /locus_tag="KPH93_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG1688343.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMVEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILS
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            12613..13542
                     /locus_tag="KPH93_001516"
     CDS             12613..13542
                     /locus_tag="KPH93_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG1688344.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            13756..15552
                     /locus_tag="KPH93_001517"
     CDS             13756..15552
                     /locus_tag="KPH93_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG1688345.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVVSIIIFIALLVIHSKVKEEETYFKSKCEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRTSLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQIAIKELLSKDDFSIHIQTLSNIIGKEQKSNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCVLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESDYLKELQNKLSKGSGVSRGVKQLNSIGNAVNLRYNQIIYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKD
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(15692..17473)
                     /locus_tag="KPH93_001518"
     CDS             complement(15692..17473)
                     /locus_tag="KPH93_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG1688346.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17496..19382)
                     /locus_tag="KPH93_001519"
     CDS             complement(17496..19382)
                     /locus_tag="KPH93_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG1688347.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19399..19896)
                     /locus_tag="KPH93_001520"
     CDS             complement(19399..19896)
                     /locus_tag="KPH93_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG1688348.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7708 a         2694 c         3504 g         6177 t
ORIGIN      
        1 atagataaat cgtataatta aatcaaatat tttaggagga taaaaatggc agatataaaa
       61 ctagaaaatg taacaaaatc ttataataaa aaaaatacag ttatcgaaaa tttaaatctt
      121 actatagaag atggctcttt tacagtattg gtaggcccaa gtggatgtgg taaatctact
      181 acgttaagaa tgattgcagg tcttgaagat ataacttcag gtaagttaaa aattgaagaa
      241 aatgatgtaa caaaaacgga tgctagtaaa agggatatag ctatggtatt tcaaaattat
      301 gcattatatc cgcatatgac tgttagagaa aacatagaat ttggtcttaa aaacaagaaa
      361 gtagaaaaag aaaaaagaga aaaacttata acagaagtta ttgatgtagt agggttgaga
      421 gaatatttag atgtaaaacc aggaaactta tctggaggtc aaagacaaag agtggcactt
      481 gctagagcaa tggttaaaaa tccaaaggta tttttaatgg atgaaccgtt gtcaaatttg
      541 gatgctaaat taagaaatca aatgagaaca gaattaatac aattacatca aaaattaaaa
      601 tctacatttg tttatgtaac acatgaccaa gttgaggcaa tgtcgatggc agataagata
      661 gttataatga acaaaggaaa gataatgcaa ataggttccc caaagaagat atatcaaaat
      721 cctaaaaatt tatttgtggc gcaatttatc gggaatcctt gtatgaacat actagatata
      781 aaaaatgatg tcaaatttgg tttcagaccg gagaaagcaa cgctagagag tataaatata
      841 gatggaaaaa atgatgatgt atttagccta tttggaaaag ttgtgactaa agaagtgttg
      901 ggaaatgaaa ctatatattg tttatctgtt cttgatagcg agataagagt caaaacggaa
      961 aatgatatat ttgatattgg aagaacttta agaataagtg tattagaaaa agatttatat
     1021 tactttgata aaaatggaga aagaatagaa gatttaaaaa tatgtaaaaa tatgtattct
     1081 aaaattaaag gagatattta tgaaaaagca atttaataaa gcatatttat atataacacc
     1141 tgctattata ggaactttat tgtttatagt ctatccaata atcaagacga tatctctaag
     1201 cttcaaatct ggaagtctcc ttagttcaac ctttgagaat gtaggcttaa acaattatac
     1261 agaactttta aagaatccag aatttataca aactataaca aatactgcaa tttatacttt
     1321 ttttatggtg actatatcta tatcactagc catagtactt gctgtttggc taaataaaaa
     1381 ttctataata cacaatttaa cgcaaagtat aatctttaca ccacatgtaa tttcactagt
     1441 atctgtagct gtattgtggc tttggattat ggagccacaa tatggctttt taaactggat
     1501 actatcgaca ttacatttac caacatcaaa gtggctttca agtgaaagtt cagctttaat
     1561 gtcactgatt ctaataggag tatggaagac tcttggatac aatatcttaa tagtgttatc
     1621 aggtcttaaa tccataccaa actatatata tgaagcatct aaattagata atgcaaacaa
     1681 gataacgaat ttctttaaag tgacattgcc actattatca ccaacaatat tttttcttat
     1741 gataacaaca acaactgcat cttttcaagt atttgatgag ataagtataa tgacacaagg
     1801 tgggccagta ggttcaacta acatgttatc ttattatata tatgagtctg gatttatata
     1861 ctatgacata gggcaagcaa gtgcagcgag tatgtttttg ttagctatag ttatgattgc
     1921 tacatatgct aactttagac tcttatcaaa gaaagttcat tatcaataaa gaaaaagtag
     1981 atattataag acttatccaa aaggaggaga aaatggatat tatacaacct atcaaaaaag
     2041 acaaagttga tataaaaaaa atagtgtcaa aatcaatgga aatattaatt ctattacttt
     2101 tatctgcaat atttatattc ccttttatat ggatgttatc gacatccttt aaaaatccac
     2161 aagaaatgat gcaattacca cctaccatta taccaaatga tatagtgtta gaaaattata
     2221 gtacagcatg gcattcaggt ccatttttta gatatttgtt aaacagtgta tttgtaacag
     2281 ggagtgtttt aattgtacaa tttttagtta tggttccagc agcatatgca ttttctaaga
     2341 ttaagtttaa aggtgaaaaa gtgttatttg ggcttttgct tgtaggactt atgattccac
     2401 cacaagtaac ttttctacca gtctacatga tgatgagtaa gtttggattt ataaatactt
     2461 atgttccact tataattcca tttatgacat cttcatttgg gatatttctg cttagacaga
     2521 attttatgca gatttcagac gagattattg aagcagctaa actagataat gcttctgact
     2581 taaaaataat gtttagaata atggttccca tggcaaaacc agcagtaata acatttatat
     2641 tatttaattt tatataccat tggaataatt atttctggcc cttagtaatg acaaatacag
     2701 atgcaataag aactttacca ataggagttg cgctccttaa atcttcagaa ggaattactg
     2761 catggaatgt aataatggca ggaaatataa ttttaatttt accaataatt cttgtctaca
     2821 tatttgcgaa taaaaaagta aaagaagctt ttatgtattc aggaataaaa taaaaaatta
     2881 aagactaaat ttggaggatg aaaatgaagc ttaaaagaaa attagcaaca ggattagcaa
     2941 taacattatt attaggttct ttagttgggt gttcaaattc taatgatgca aatgcatcta
     3001 aaggagaaaa gactgatagc aatattacga agatagattt ctgggctcct ttaggtggaa
     3061 ctaatggaga aactgctcaa aaaatggttg accaatttaa tagtgaacat aaggatgtac
     3121 aagtaaacat gttaaaacag aaagattact atgaaaatgc aacaaaacta caggcagcct
     3181 taacatctaa agaccaacca gatgtaacat tattagaaat aactcaaaca ggtacattcg
     3241 catcagcagg tgctttagta gatatgagta aatattttga taaaacatat caagaaagat
     3301 tcttttctgg tcttttaaca aattcatatt atgaggataa gtttgttggc atgccattta
     3361 atagaagtac accaatatta tacataaata aagatatggc aactaaagca ggtcttgacc
     3421 cttcaggtcc aaaatcatgg gaagagctta aggattatgc tagtaaaatg acaaataaat
     3481 cagaagatac ttatggcttt gaaacaccaa tagatatttg gttctatgaa gctatggtaa
     3541 tgcaaagtgg cggagaaata actaatggta aaaaagtagc atttaataat gaagcaggac
     3601 aagcaccagt taagttctgg caagatatga tgaaagctgg aataatgaaa atgcctcctg
     3661 gagaagatta taatgcttgg gatgtagcaa aacaagattt tgtaaatggt aaggtaggta
     3721 tgatatttac atcaacagca gatttagcag gacttatgca acaaacagaa gggaagtttg
     3781 aaatatcaac agcattctta ccaaaaaatc aaaaatatgc aactcctaca ggtggggcta
     3841 atctagttat gcttgaaggt ggaacagata aagaaaaaga agcaagtgca gaatttatag
     3901 aatggatgac acaaacagat aaaatcgttc agtttagttc atcaacagga tacttaccga
     3961 caactgaaga tgcaacaaac tcagaaaagt tacaaacatt atataaagaa aagcctcaat
     4021 ataaggttgc aactgaccaa ttacaatatg ctgttgcact tccaatgtta aatggataca
     4081 aggaagcaac tgataagcta atggatgaga ttaaaaaggg gcttactgat ttaaatgcaa
     4141 atccaaaaga tgtagtaagc aaaggaactt ctgcaatgca agcagtaata gataaaacaa
     4201 ataaataatt atatagactt gcaagttgat taaaagtatt caataatata taaatgaaga
     4261 ggtaatgtaa atgacaaagg ttatagtatt atcagtagat tctttatttg aaaaagactt
     4321 ggaatttgta aaaaatctac caaactttaa atctatacta gaaaattgtt ccatagtaaa
     4381 aaatataaat tgtgtttatc ccactctgac atatccatgt catactagta tggttacagg
     4441 agtatatcct aaaaaacatg gtatatatca taatgaaaaa tttgacccaa ataaagaaaa
     4501 taaagactgg tactggtatt caaaggacat aaaagtaaaa actattatag atgtagcaaa
     4561 agaaaataat ctgactacct ctagtgtatt atggcctgta atgggagcaa atccaaatat
     4621 agattataat attgcagaaa tatgggctcc tagtagagaa gatgacccaa gagaaacttt
     4681 tgaacgaagc tccagtaagg ttataatgga cggtatttac aatagacatt gtcattatat
     4741 tgattggaaa ttagaaccca acatggattt atttggagta aactgttcag tagatataat
     4801 aaaggaatat aaaccagatt taatgttaat acatttagct acattggacc atacacgaca
     4861 caattatggc ttatttaatg acgaggtaga taaagcatta aaaatgaatg ataaatggct
     4921 aggagatatt attcaggcta caaaggatgc aggtacatat gaagatacaa attttataat
     4981 acttggggac catggtcatc ttagagttga taaagttata aatccaaatg tcttgcttaa
     5041 atcaaatgga tttataaaag tcgaaaatgg agaagttaag gattttgatg cctatgtaaa
     5101 ttcttctgga atatcagctc aaataatagt aaataactta gataggttaa cagaattaag
     5161 agaactgctt gaagaattta aagaagaatt attcatagag aatattttta ccaaagagga
     5221 agcatccaac cttggtcttg aaggtgattt tgaaatggta attgaaggtc ttgaaggaat
     5281 ttcatttgga aatgatttcg aaggaagtat tataaaagat tcagatataa aagattacaa
     5341 gtttgctgtt gcgacacatg gtcatctacc tactaagggt aataggcctc catttatagc
     5401 ttttggacca aatattaaag gtggtatggt tatagaggaa ggagacctga gggcgcatgc
     5461 agcaactata ttaaaaatgt ttgatttaaa gttagatgga gttgaaaaag aagcatttga
     5521 ctttattata taagttgaat aaagcattta ttttccatga attatagact tctattgcaa
     5581 agataggagt ctttttttat gttttagatt taagtaaaat ttaaatgggt aaaatattta
     5641 aaataactta aacaatttta taaaggtatg atataataat acatgttagg aactaatgtt
     5701 cggaaaaagg agtgatacta tggattttaa aataaaatca gacttcaaac ctacaggtga
     5761 ccaaccagaa gctattaaat caatagtgga ttctataaat agaaacgaga aattttcgac
     5821 attattaggt gtaacgggtt caggaaaaac attcacaatg gcaaatatca ttcaacaagt
     5881 taagaaacct accctaataa tggctcataa taagacacta gcagcacaac tttacagtga
     5941 gtttaaagaa tttttcccag acaatgcagt tgaatatttt gtaagttact atgattacta
     6001 tcaaccagaa gcatatgtag ctcacagtga cacttatata gaaaaagatg caagcataaa
     6061 tgatgaaata gacaaacttc gacattctgc tacagcatct attttagaga gaagagatac
     6121 tataataata tcttctgtgt cttgtatata tggacttggt gacccgaaat actataaaga
     6181 acttatgtta tccataagac caggtatgca aagagataga gatgatgtaa taaaaaggtt
     6241 aatagagata cagtacgaaa gaaatgacat aaattttaca cgaggtactt ttagggttag
     6301 aggagatata ctagaaatat tcccagctag taacgatgaa aaagcaataa gaatagaatt
     6361 ttttggtgat gaggtagata gaataacaga gattgattat gtaacaggga agattgttgg
     6421 tactagaaat catgttgtta tattcccagc gtctcactat gtaacaacac cagaaagaat
     6481 tgaaaaagca attgtagaaa tagaagacga gcttcaagaa caaattaaat ttttcaagga
     6541 aaatgatagg ctcttagaag ctcaaagaat tgaacaaaga actaagtacg atatagaaat
     6601 gttaaaggaa ataggatttt gtcaggggat agagaattat tctagacata ttacaggaag
     6661 gtcagaagga gaaaggccat atactttaat ggatttcttc ccagatgatt atttgataat
     6721 tgtagatgag gcacatgtta ccataccaca agtaagaggt atgtatgcag gagacaggtc
     6781 tagaaagaca tctcttatag agaatggttt tagattacct tctgcacttg acaatagacc
     6841 attaaatttt caagaatttg aaggaaatat aaatcaaatg ttgtttgtaa gtgctacacc
     6901 aggaccttat gaaattcaac actcagagac aatagcagaa cagataataa gaccaactgg
     6961 ccttttagac ccaattgtag aagtaagacc tataaataat caaatagatg atttggtagg
     7021 agaaataact aaaactatag aaaaaaatga aagagtgctt ataaccactt taactaaaaa
     7081 aatgagtgag gacttgacta attatcttaa agaaataggg ataaaagtta aatatttaca
     7141 ctcagatata gttacattag aaagaacaga aataataaga gatttaagat tagggaagtt
     7201 tgatgtcctt gtaggaataa acttacttag agaaggattg gatataccag aagtttcact
     7261 tatagcaatt ttagatgcag acaaagaagg tttcttacgt tccgaaacag ccttaataca
     7321 gactattgga cgtgcagcta gaaatgaaaa tggaagagta attatgtatg cagacaggat
     7381 aactgactca atgcaaaatg ctattgatga aaccaagaga agaagagata tacaaaattt
     7441 atataatgaa gagcacaata tcattcctaa aactatacag aaaaatataa gagatagtat
     7501 agaagctact aaggtggcag aggaagaagt tgtatatgga atatctgata cagatgataa
     7561 agacgaaata agagctaata tagataaatt aaaatcagag atgatggaag ctgctcaaaa
     7621 cttacaattt gaaagagcag cagaacttag agataaagtg aaacaattag aagaaaaatt
     7681 ggaaaaatag aaaggtaaaa acaacattat ggaagataaa attataataa gaggagcaaa
     7741 agagcataat ttaaaaaata tagatttaga attaccaaga gataaattta ttgtttttac
     7801 aggtttgtca gggtctggga agtcttcttt ggcatttgat acaatatatg cagaagggca
     7861 gagaagatat gtagaaagtc tatctgctta tgcaagacaa tttttaggtc agatggaaaa
     7921 gccaaatgta gaatatatag agggtttatc accagctatc tcaattgacc agaagactac
     7981 ctcaaaaaac ccacgttcta ctgttggaac agtaacagaa atatatgatt atttgagact
     8041 attatttgca agagtaggag atgtatattg ccctacatgt ggtgaaaaaa taagtcagat
     8101 gactattcaa gaaatagttg ataaaatgct agattttcca gatagaacga aattacaaat
     8161 tctttcacca attgttagag gacaaaaagg cacacataaa aaagtttttg ataatatcaa
     8221 aaaagaagga tttgtaagag ttagagttaa tggtgaaaac tatgaagtta gtgatgatat
     8281 agtcttaaat aaaaatcaaa aacataatat agaagttgta gtggacagga tagtaattaa
     8341 agatggaata gaatcaagac tagcagattc aattgaaact gcagttaagc tatctgatgg
     8401 tcttgtaata gcccaaataa tagatggaga agaaattctt ttttcaacta aatttgcatg
     8461 cccagaacat ggaattggta tagaagaatt atctccaaga atgttttcat ttaatgctcc
     8521 ttttggtgct tgtgatgttt gtaatggtct tggggaaagt aaagaagttg acccagaatt
     8581 agtagtacca aacaaagatt tatcaataaa acaaggtgca gtggcagcat ggggttcaac
     8641 aggtgttaat gatgacactt attacagtaa aatgataaaa agtttagcag agcattttaa
     8701 tgtatctttg acaacaccat ttaaagattt accagatgat tttgtggagg aattacttta
     8761 tggtaaagat aatataattg tagagtttac ttatgagtca aaatttggag gaactagaaa
     8821 ttataaatca tactttgaag gtgtaatagt aaacttagag agaagatata gagaaactaa
     8881 ttcagattac atgagggata agattgaaga atacatggca gaaagaccat gtccaaagtg
     8941 taaaggtatg agacttaaaa aggaagtttt atctgtttta gttgatggaa aaaatataat
     9001 ggaagttaca aacttatctg tgaatgaatt gattgacttt atggaaaata taaatcttac
     9061 agaaaaacag aggtttatag ctcatgagat tataaaagag ataaaaggaa gagctaagtt
     9121 cttaagagat gttggtcttg attatttaaa tttatcaaga aaagctggaa ctctatcagg
     9181 aggagaagca caacgtataa gattagctac tcaaataggt tctgcattag ttggagtatt
     9241 atatgtactt gatgagccaa gtatagggct tcaccaaaga gataatgata ggcttataac
     9301 tactcttaga catctaacag acttaggaaa taccttaatc gttgtagagc atgatgaaga
     9361 cacaatgaga gaagctgatt atgttataga cattggtcca ggtgcaggta tacatggtgg
     9421 tgaaatagtt gcacagggta ctttagatga aataattgaa aatgataact caataacggg
     9481 tcaatattta agcggtaaaa aacaaatatt gcttcctgag actactagag aaggaaatgg
     9541 aagttttata gaaatagtta aagcatcaga aaataatctg aaaaatattg atgtaaagtt
     9601 cccattaggt aagtttacat gcataacagg cgtttcaggt tctggaaaaa gtacgctaat
     9661 aaacgatata ctttacaaag gtgttgcaag taaagtgaat agacttaagc aaagagctgg
     9721 taagcataag gagataaaag gaattgaaag tatagataaa gttataaata tagaccaaag
     9781 tcctattgga agaacacctc gttcaaatcc tgctacttat acaggtgtat ttgattttat
     9841 aagagatatt tttgcatcta ctaatgaagc taaagcaaga ggttacaaaa aaggtagatt
     9901 tagttttaat gtaaaaggtg gtagatgtga agcttgtaag ggtgatggga taataaaaat
     9961 agaaatgcat ttcttaccag atgtatatgt tccttgtgag atttgtaaag gtgaaagata
    10021 caatagagaa acattgcaag taaaatacaa agataaaact atatctgata tattagatat
    10081 gaatgtggaa gaagctgttg agtttttcga aaatatacca aatataaaaa gaaaattaga
    10141 aactttaatg gatgtaggac tttcatatat taaattagga cagccttcaa ctcaattatc
    10201 aggaggagaa gcacaacgta taaaattagc tgctgaactt agtaaaagac caacaggaaa
    10261 gactttgtat atattagatg aacctactac tggacttcat atggcagatg tagacaagct
    10321 aattcaagta ttacaaagac tagcagatac aggaaataca attatagtca tagaacataa
    10381 tttagatgtt ataaagactt gtgattatgt aatagactta ggaccagaag gcggagataa
    10441 gggtggaact atagttgcaa ctgggacacc aaaagaagta tctaaagttg atggttctta
    10501 tacaggtcaa ttcttaaaga aatatttttc ttaagttata taaattgtga aaaattttag
    10561 ggctagatat atatgataaa ctgttctgtt ataagtaaat tagtcaaata caagttgtaa
    10621 atagtaatct aaagtaaagt ccatattact cttcatggtt aaaattgttt tcaaatcaat
    10681 tatagaaggg aatatggact ttaacttgtc taaagcactg gaaattgtat aactttttaa
    10741 atttcatatt ttagggtata attttagtat aaaatttaat gttttggagg gcgagatgtt
    10801 tgatatacaa gagcatttaa aaaaattacc ttctgagccc ggagtatatt taatgaaaga
    10861 caaatacgac catataatat atgtgggaaa agccatatct ctaaaaaata gggtaaggca
    10921 gtattttcaa tcttcaaaaa accatacatc taaagtaaaa tctatggtta agaatatata
    10981 taagtttgag tatattataa cggattcaga gttagaggca ttaatacttg aatgtaattt
    11041 aataaaaaga tacagaccaa agtataatgt agtacttaga gatgataaaa cttatccata
    11101 tataaaagta actacgaatg aagattatcc gagaatctta aaagttagaa gagttcttaa
    11161 agataaggca aagtattttg gaccctatac caatataact gctgtgaatg atactttgga
    11221 gcttataagt agtacgtatc caataagaag ctgtaaaata gatatagata aagccataaa
    11281 gaataagaca agaccttgtt taaatctgca cataaataaa tgcttaggac cttgcacagg
    11341 aaatgtttct aaagaagagt atggaaaaat ggtagaagag attataatgt gtttatctgg
    11401 taaagaagaa aaattgatgg aacttttaaa ggaaaaaatg aatgagagtt ctatgaactt
    11461 tagatttgaa gaggctgctg tatacaggga taagataaag agccttgaag agatgataca
    11521 gaaacaaaaa atagatgcta ctgtaagtga cttaaaccaa gatgtggttg caatggcaag
    11581 agctcataat gaagcttgtg tccaggtatt ttttataaga aatggtaaga ttgtaggtag
    11641 ggaacatttt atattagaag gtgtaatgga tagtccaaga gcttctatat taagttcatt
    11701 tgtaaaacaa ttttataatg aacaagagta tatacccaaa gaattaatta tagaagatga
    11761 gatagaagat agctctatat tagaagaatg gttgtcatct aaaaaggggc aaaaagtaac
    11821 aataagagtt cctcaaaagg gagagaaaaa aagcttagtt gaaatggtca gaaaaaatgc
    11881 tgtagaatat cttgaaaaat tttctgatat gaataaaaga aaatatgaaa aaagtattgg
    11941 agcattggaa gaattaaagc aaatattaag tctagaaaag ttgccaataa gaatagaggc
    12001 ttatgatata tcaaatatac aaggtgtaga ttcgattggt tctatggtag tgtatacgaa
    12061 tgctaaaaaa gataagaagg aatatagaag atataaaata aagacagtta ttggacctaa
    12121 tgattatgat tctatggctg aaattgtaga tagaagatta aaacatggaa atttacctga
    12181 cttgatattg ttggatggtg gaaaaggaca agttagtgca gtaaaaaaag tactagaact
    12241 taatgatgta gatatacctc tttggggaat gtacaaagat gataagcata gaaccaaagg
    12301 attgatatgc aaagaaaaag aaatagagct agataaaaca actaatttat atagatttat
    12361 tgctagtata caagaagaag ttcataatta tgcaataaca tatcatagaa gtctaagaaa
    12421 taaagctttg acaaaatcta tcttagatga tatacaagga ataggtgaaa aaagaaagaa
    12481 atcattatta aatcacttta aagatgtaga tgctataaaa aaagctacta tggaagaact
    12541 tttagaagta gatggaatga ataaaagcat tgcagataat gtttataatt tttttagaaa
    12601 ggaagaaaat taatggaaac tagcaataaa gtatcaataa gagagataat aaaagagttg
    12661 aagttaaaag tagtatacat gcctgatgat gtagattatt atgtctactc tcaagatata
    12721 aatagaccag gattacagtt tgcaggatat tttgattatt ttgcatatga aaggatacaa
    12781 atagttggaa agacagaata taattttttt ggattaatgg atagtcatat tcgagaagaa
    12841 gtactagata gatttttttc atatgaaata ccagcgttaa taatttctag ggacttggaa
    12901 atcaagccag atgtaattga aaaggctaaa aaatataaaa gaatactttt gagcacaaat
    12961 agaaatacca ctagacttat aaatagactg tcaaattatt tggataataa gttagcacca
    13021 cacacaacaa ttcacggggt gctagtagat atttatggta taggtgtact gataaaaggt
    13081 gaaagtagta taggaaagtc agaaacagca ttagaactga ttcaaagggg taatagatta
    13141 gttgcagatg atgctgtaga aataagaaaa ttagatgaaa gtatgcttgt gggtcagtca
    13201 cctgaactaa taagacattt tcttgagata agaggaatag gaataataga tgttagaagt
    13261 ctatatggag taggggcaat caaaaattct aaaatgatag atttagtagt tcatttagaa
    13321 gcttgggatg aaactaagta ctatgataga cttggactgg ataaagaata tgaagaaata
    13381 cttggtgtta atgtagaaaa attggttgta cctgttaaac ctggaagaaa tactgctatg
    13441 atattagaag tagcagcaat gaattttaga caaagaggaa tggggtatga tgcagctcaa
    13501 gaatttacta aaaaattatc aaaattaatt gatactaagt agtactgaat aatagtgaac
    13561 tacacatcat ttaattaaat attgattagg attgtgtcct acaccttata aattaaaatc
    13621 aagttgtata tatataatgc ttattaataa gatatattat ataaggataa tttaaagtac
    13681 aatgctaggt tataataata ctagattaat tttatatttg aatttcatta tgtaataaga
    13741 aagttggaat tttttatggg aatagaatat tttagtaaac ttttagaaag tagtactgaa
    13801 aaagaattgc tcttaagcaa aaagtcaaat aacataagta cagcaagatt aatatctttt
    13861 ttgatagtga tagcagggct tgctattgga ttttacaata aaaatatagt aggtattttt
    13921 gttggagttg tatcaataat aatatttata gcactcttag ttattcatag taaagtaaaa
    13981 gaagaagaaa catattttaa aagtaagtgt gaagtattaa ataagtatat taagcgattt
    14041 ggagatggct ggaaggaatt taaaatagat ggtaaagaat acttaaaaga tgaaaactcg
    14101 caagcaaaag atttagactt atttggaaga acatcattgt atcaatatat ctgtgtagct
    14161 aatacatctt acgggaaaaa ttttttagcc aaatatttgt ggaatgaaaa tccagatgag
    14221 aatgtaatct tagaaagaca gatagctata aaagaacttt tatcaaaaga tgatttttct
    14281 attcatattc aaacactcag taatataatc ggtaaagagc aaaaaagcaa ttcagatagt
    14341 agtatagaat cgtttataga gtatggagaa aataagaatg tgtatatacc aaaatggatg
    14401 catattttta cttggggttt accaactgcg acaatcttat cttttatatt ttgtgtgtta
    14461 ggatttttac ctgttttacc tgttttttta ctttttataa tacagttagg atttagtggt
    14521 tttggatatc caagacttat gcaaactctt tctccattat tttcatttag tagaagtata
    14581 caagtatatg aaaaaatgtt tgaggtttta gaaaaagaga cgtttgagag tgactatttg
    14641 aaagaattac aaaataaact ttctaaggga agtggagttt caagaggtgt taaacaatta
    14701 aattcaatag gaaatgctgt aaacctaagg tataatcaga taatatatat aattgcctgt
    14761 ggtgttttga tgtggaacta tcattgtgca gaagctttgg agcgatggaa aggtatatat
    14821 ggaaatcaaa ttagagattg gtttgaatct attggggaat ttgaagctct aataagttta
    14881 acagttattt ctcatgtaaa ggaaaataca tgttttccag taataaaaga tgaagatact
    14941 ccaagattga aagttgaaga ggtataccat ccattaatag cagaaaaaag tgtcatagca
    15001 aattcgatac aattaaattc tcagacttgt attatcacag gttcaaatat gtctggaaag
    15061 acaacatttt taagaagtat cggtgtaaac ttagtgctgg catatgcagg tgctccagta
    15121 tgcgctaaga gttttgatgc gacctgtatg gctatattta cttctatgag aatacaagat
    15181 gatgtaagtc aaggaatatc aactttctat gctgagattc ttagaataaa atcaatgatt
    15241 caatatagtt taaaagaact gccaatgttg gttctagtag atgaaatttt taagggaact
    15301 aactctgctg atagaattat tggagcaagt gaggcggtaa agaaattatc aaaaccctgg
    15361 gttataagta tggtgacaac acatgacttt gagctatgtg acctttcagg tagcggagat
    15421 gttgaagttg taaactatca tttctcagag tactatgtag acgataaaat tcattttgac
    15481 tataagataa aagatggaag atgtaaaact acaaatgcaa aacaacttat gagaatggca
    15541 ggtattcttt aattaataga taggttaatt aataaataag tctatttaga aatataattt
    15601 aatttttgaa attaataagg attgtcttaa attacgtaaa agtattttaa gacaatcctt
    15661 ttttaagaat aaattttata ctttgttatg actacttagc ttcttcacaa gcacattctt
    15721 gtggacactc tccatagaca caactcttat caaagtaatg agtatgaagg tatttatgag
    15781 ctacatcact atttggttct ccaaagaagt ctttataaat agcttgtaaa tcaggattct
    15841 catgagattt acgaatagcc ttatttctat ctatttcata taacgcattt gcacgtctat
    15901 caactatttc agtattacca tggtgataag gttgaccagc acctccaaca catccaccag
    15961 gacatgccat aacttctatg atatggtatt tacattctcc acttctgacc ttatccataa
    16021 tctttctagc gtttttaagt gtatttacaa tacacacatt aactgtagtt ccatcaacat
    16081 ctatacttgc ttccctaatt cctttaagac ctctaacatt agtgaagttt acattatcta
    16141 aagtcttatt agttatcttc tcatatgaag tacgtagggc agcttctaaa actccaccac
    16201 ttgctccaaa tatagatgca gcacctgtag attttcctaa aggattatca aaatcttggt
    16261 cttgaagatt aggtaaatcg atagcagctt cttttatcat cttagctaac tctctagtag
    16321 ttatagaaag gtcaacatct agtattccag attcacttaa ttcttctctt gaagcttcat
    16381 attttttagc tacacaaggc attacagata caacataaag ttcttcagga ttgattccta
    16441 atattttagg tgcatagtag tttttagcta tagaaccaaa cataccttga ggagacttac
    16501 agctagatgc taagtttaac ttgtctggat aattgtgctc taagaagttt acccatgcag
    16561 gacaacaact tgttaatata ggaagatttt cacctttttg gattctttct ataaattcag
    16621 tagcttcttc cattatagta aaatctgcac caaagtttgt atcaaataca tgttcaaatc
    16681 caagtgcttt taaagcagct accatttttc ctgtagagat tgaccctggt tctaatccaa
    16741 actcttcacc aatagcaact cttacagcag gagcaacttg cactactata gtcttttctt
    16801 ttttatttaa aacatcccaa agtttaggta cattatctac ttctgttaat gctccagttg
    16861 gacagacaga tatacattga ccacagaatg tacactctgt ttcaaccata tcagagttaa
    16921 agaatgtact aactaaagta ttaaatccac gattaactcc agataatgct cctactgttt
    16981 gtatgtcatt acatacagtc tcacatcttc tacataatat acacttagaa tggtctttaa
    17041 ctaaagattt agtagaagta tctttacctg caaaagattt ggaaccttga tatcttattt
    17101 ttcttactcc taaatcagca gctatagtct gtaattcaca atctccattt ttttcacaga
    17161 caaaacaatc ttgtggatgg tctgataaca ataattcaac aatagttcta cgagcattta
    17221 atgctttagc actattagtc tgaactttca taccttcctt tataactgtt ccacaagctg
    17281 gaactaatcc tctctctgtc tcaaccatac aaacacgaca agaagcacaa gtgtcaagct
    17341 tatctatttc attcatatgt aaatgacaaa ggttgtgtat ttttatattt attaatttcg
    17401 cagcatctaa gatagaagtt ccactaggtg ctgaaacgtg tttgccatta atagttaaat
    17461 taactaaact cattgttatt cctcctccaa ataagttatt tcttgattat agcgttaaat
    17521 gtacaattat ctatacaagc accacatttt agacacttag aagtatctat tgtatgttgt
    17581 tccttaacag aacctgttat acaaccagca ggacacactc tagaacattt tgtacaaccc
    17641 ttacagtcat ctgttataaa gtaagaaagt aagttctgac atttaccagc aggacatttc
    17701 ttatctacaa catgagctat atactcatct ttgaaatatt gaagagtact taaaacaggg
    17761 tttggagcag ctttaccaag accacataat gaagcagttt gtatagtttc agcaaggttt
    17821 tctaggtcat ctaaatcttg tagagtacca ttaccttctg taattttagt taatatttct
    17881 aataatcttg tagtacctat acgacaaggt gtacatttac cacaagattc ttcaacagta
    17941 aactcaagga agaacttggc tatatctacc atacagtcag attcatcaag aacaagcata
    18001 ccaccagagc ccatcattga accgatagaa cttaatgaac caaagtctat aggagtgtct
    18061 aaatgttcag ttggtataca tccaccagat ggtccacctg tttgaacagc cttaaatttc
    18121 ttaccttcag ggattccccc accaatttca aatacaatat ctcttaaagt agtacccatt
    18181 ggaacttcaa caagtcctac attttctact tttccaccaa gagcaaagac tttagtacca
    18241 ctagaatctt cagtacctaa gttggcaaac caatctccac ccttaagtat aattgcaggt
    18301 atgttagcaa aagtctcaac gttatttaag caagtaggag atttccatag accactctta
    18361 gaagaactat aagttttcat tctaggttca ccacgtctac cttcaattga atgcattagc
    18421 gctgtacctt caccacaaac gaaagcacct gcaccatatt ttaattctaa tttaaagtta
    18481 aatccagtgc ctaagatatt ttcgccaagt aaaccgtatt tttcagcttg agctatggct
    18541 acttttaatc tttctataga ttttggatat tctgctctta tgtatatgta tcctgtatca
    18601 gaacctatag cgtatccaca tattgccata gcttctagta cactatgagg gtcaccttca
    18661 agtacagatc tatccataaa tgcacctggg tcaccttcat cagcattaca tacaacaaac
    18721 ttcttatctg ttggaccagc tggatttttt gaagcagctt cccatttaga gccagtaggg
    18781 aaacctgcac cacctctacc acgaaggcca gaagttttta cctctgcaat aacttcttga
    18841 ggagtaagag aagttaaaca tttgcctaga gctaaataac catcattggc aatatattct
    18901 tctatactat ctgggtctat aagaccacaa ttttttaaag ctatacgtaa ctgttttttg
    18961 tagaaagaca tttcttcttg ttcttcaact tttttactta aagattcttc ttcatataat
    19021 aattcctcga caactgtatt tctaattaag tggctttgaa ctatcttttc agcatcagat
    19081 ggctcaactt ttacatagaa aacgttatca ggataaactt ttacgatagg accttgagca
    19141 cagaaaccaa aacaacctgt caaacgaaca ctaactttat cttggattcc tgatttcttt
    19201 atttcatttt ctaattcact aactatttct agactgtttg aagatgtaca tccagtatca
    19261 caacaaacaa gtaattctct tcttaataca ttttccttag aataattttt tcttaagcta
    19321 agattaggtt tcagctcatc agctaaaact tttaattcat caaatgaatt tactttacgc
    19381 attatttatt cacccctctt aacaattcaa ttctaattct ctataagtat ctaatatttc
    19441 gcttacttgg tcaggtttca ctttaccata aactttacca tttacagtaa caactggagc
    19501 taaaccacat gcccccaaac atcttaaacc ttctaaagaa aatttaaaat cactagtagt
    19561 ttctccaact tttatgccaa gctgtttttc aaaagcactt aagattttgt cagcaccttt
    19621 tacaaaacat acagttccta gacagacact gattttatac ttacctacag gttctgtagt
    19681 aaaataagaa tagaatgtaa caaccccata tacctttgca ggagctactc caagttttct
    19741 agcaacatga agctgaactt cttttggaag atatccaaat ataccttggg cttcatgtag
    19801 aacttgtatt aacgcacctt cctttgttgc aagagagtca ataaatatat ctagttcgtc
    19861 aaataattgc ttgttgtgtg aaataaaatc gcacataaca aatccccctt ttctttttaa
    19921 agttattatt tttcataaaa taattaacca tgagttgacc ccaacattat gtgggtgaac
    19981 aaaaccaaaa caataaagaa agaataatat acaacaattt ctttatttat aaaaacaatg
    20041 caaaaaagtt acctattaaa aataaaaact atactagtct att
//
LOCUS       DAEMAE010000053        19602 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEMAE010000053 DAEMAE010000000
VERSION     DAEMAE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.054"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(367..588)
                     /locus_tag="KPH93_001521"
     CDS             complement(367..588)
                     /locus_tag="KPH93_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG1688349.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVIKSKSFANVKAEALNED
                     VYAVAEAIASLQEKPIVDILKLDSTSLSK"
     gene            1168..1425
                     /locus_tag="KPH93_001522"
     CDS             1168..1425
                     /locus_tag="KPH93_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1688350.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVTKLYGGTNTYIPTYKGL
                     FRYARNREIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1895..2200
                     /locus_tag="KPH93_001523"
     CDS             1895..2200
                     /locus_tag="KPH93_001523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688351.1"
                     /translation="MNNGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFN"
     gene            2225..3133
                     /locus_tag="KPH93_001524"
     CDS             2225..3133
                     /locus_tag="KPH93_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG1688352.1"
                     /translation="MELDCVCNDEFIDNSGIEFDCNSKDNSFNGDINVETEHCINEYL
                     YDDDVEHNYNNFHNEDTIKNKAISNIRTIKKYIDESFDTNKSENSNLQQESINFNFND
                     TCNEIDKRTNNSIENNQNKRSMRLSDMNVLSDKGKTNKKNYFNHNHGIQKGTKSVTKK
                     KESLNKFNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEV
                     KVAKWKNTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKESNSNDSSNYIHSD
                     YTTYDGSVRERKKYENVRYWNPICEI"
     gene            3532..4833
                     /locus_tag="KPH93_001525"
     CDS             3532..4833
                     /locus_tag="KPH93_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1688353.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDKKSLAIKTSDVTNIITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            4909..5184
                     /locus_tag="KPH93_001526"
     CDS             4909..5184
                     /locus_tag="KPH93_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1688354.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELIIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            6403..6633
                     /locus_tag="KPH93_001527"
     CDS             6403..6633
                     /locus_tag="KPH93_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1688355.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            6626..7207
                     /locus_tag="KPH93_001528"
     CDS             6626..7207
                     /locus_tag="KPH93_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1688356.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMMLFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(7766..8443)
                     /locus_tag="KPH93_001529"
     CDS             complement(7766..8443)
                     /locus_tag="KPH93_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1688357.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(8632..9996)
                     /locus_tag="KPH93_001530"
     CDS             complement(8632..9996)
                     /locus_tag="KPH93_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1688358.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSILPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGMGVRGASIATAISQIASTLVYIGYIL
                     SKKSIFSFRISECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLIVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVMLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGICFIPIILIIPVFYGLNGILYAQPIADLIAAIIAGFMSIRLHKELNLLKI
                     NCMH"
     gene            10124..10645
                     /locus_tag="KPH93_001531"
     CDS             10124..10645
                     /locus_tag="KPH93_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1688359.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSRPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            11025..12104
                     /locus_tag="KPH93_001532"
     CDS             11025..12104
                     /locus_tag="KPH93_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG1688360.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKYTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVAGFYFAFFFPDFVEVYGQDDVNWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKNEHVRRLASEGCRPQLPW
                     GQAIAKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVVSIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDATSINIQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYVKFNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            12427..12795
                     /locus_tag="KPH93_001533"
     CDS             12427..12795
                     /locus_tag="KPH93_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG1688361.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEQFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            12912..13715
                     /locus_tag="KPH93_001534"
     CDS             12912..13715
                     /locus_tag="KPH93_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1688362.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGIDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            14107..14997
                     /locus_tag="KPH93_001535"
     CDS             14107..14997
                     /locus_tag="KPH93_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1688363.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILKKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRGISPKLVFKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKMSESYTQIKFF"
     gene            15311..15754
                     /locus_tag="KPH93_001536"
     CDS             15311..15754
                     /locus_tag="KPH93_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1688364.1"
                     /translation="MIEIKTIEINKKGQIDLLMLADPCEEMIDKYLNKGTMYALYDNE
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYYLLDVYSKKYSSMIVGTAES
                     GVSFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            15926..16096
                     /locus_tag="KPH93_001537"
     CDS             15926..16096
                     /locus_tag="KPH93_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688365.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            16469..17566
                     /locus_tag="KPH93_001538"
     CDS             16469..17566
                     /locus_tag="KPH93_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG1688366.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTVIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKPFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            17771..18259
                     /locus_tag="KPH93_001539"
     CDS             17771..18259
                     /locus_tag="KPH93_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG1688367.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSLIVEDFDIPYKWMYSQMI
                     ERIPKFDDSMIWVWSKRPDLRFSAYLERGLKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVEIYEDEQIEKQVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVRLIKEFV
                     AK"
     gene            18345..18752
                     /locus_tag="KPH93_001540"
     CDS             18345..18752
                     /locus_tag="KPH93_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688368.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPVI
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDVNVS
                     NLNIGMSLLVILLIQSLLKLILFIYFDRKGIYN"
     gene            18753..18971
                     /locus_tag="KPH93_001541"
     CDS             18753..18971
                     /locus_tag="KPH93_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688369.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFVEENNI"
BASE COUNT         7770 a         2006 c         3349 g         6477 t
ORIGIN      
        1 ttagtatttc aattgtcaat attagtgttt tgcttgttag tattaatatt tcaattgtca
       61 atattagtgt tctgtttgtt agtatcagtg ttttaattgt tgacactagt tattgctgat
      121 atagaaaata tttaatttat ttcaattaat tagattgaag tagggtagca tttaaaatag
      181 ctaacatgtt taagttgaaa tacatttaat atattaattt ttaaagtatc aatatattaa
      241 agttatattc taaattgaag ttataacctg ccaaatctac agagtattaa gtgcgttata
      301 taacttaatc ttttatataa agagttaatt tttttaattt gtaaagcaga ttacgtttaa
      361 aagatattat ttagatagtg atgtactatc taattttaaa atatctacaa taggcttctc
      421 ttgaagactt gctattgctt ctgcaacagc ataaacatcc tcatttaatg cctctgcttt
      481 tacgtttgca aatgatttac ttttaataac tgccttaccc ttatcatcaa aaccagcatc
      541 caattttagt tttatactag ataaattttt tgttgaaaca acttccataa aattacctcc
      601 taaaaattta atttcaaaga tttgtcgact ttctttgtta gctataatac tatcatctaa
      661 gataatatcg gagttaaaca gtaatttcta aagatttttt gtgtaaattt ttataggaaa
      721 aattttactt ttttgatgat gaaaaaattt ataagttatt tgtgaagata tgttgttagt
      781 gttttgaaat agtatttgca tatattccta tttagttgag gtagttttgt atggattttt
      841 atatgtgtag agttaaatgt tagatgctaa agtttatgtt aaattaaatg ttaaagttta
      901 tgttaaaaat tttattaaat ttcatctgta aataaaaaag ttaaaagtcc aatattaaca
      961 ttttactttt aaaccgttaa aatctttatt aaagctaaca aagttataag taatgaaaaa
     1021 ttttattaaa gttaatacag aaattaattt taaagctaag aaatttttta ttagattcaa
     1081 taaaactaat aaatatagtt tagtcaataa tgtattaaat aaaaactttg ttaagtctat
     1141 aaaaatatct taaaggaggt gtaacttatg aagataaaag tggaggatat accagttcaa
     1201 tttcatgcaa tggtagaaat aatcggaatt gataagttca tagaggttac caagttgtat
     1261 ggaggaacaa atacttatat acctacgtat aagggacttt ttagatatgc tagaaatagg
     1321 gagatagtaa agcaatttaa tggagtcaac cacaatgaat tggctattag atataatatg
     1381 tgtgtaagca atattaagcg aatactcaat gaaaatagtg tttaaatata ataaaaataa
     1441 tgtttaaaca taatgaaaat agccttatta aatgtaagag taaagacaac gaagatgttt
     1501 tataaagttt ttttagacaa attaactaaa tttattactt attaatttaa atacattgta
     1561 tattctataa aaaaatttgt tagatactgg agatatattg agaatacaaa gaaaatagtc
     1621 gaaaatattt gttggctaaa aaatagataa aatgatacaa ttgcttgtat aaaggggaaa
     1681 aataaataaa taaactatat aaataaagaa atagcaatta atatgacata atatatttat
     1741 aaaattaaga atataaatag atattttaag ggattagttg ttaaattaga aaatattaaa
     1801 aaaaatatat atttatattt aaactatttt gttatttata taaaaatact tatctagtta
     1861 ctagcaatta ttagtagaaa tgggggaaat agagatgaat aatggatttt acaaattaaa
     1921 ttcagtggta ttgaactttg atattaagaa tgatgttaac aagataagat ttaatcatta
     1981 tataatgaga aaagaaaaca tacaaagact tcaaggaact ctgccaaacg gacaatttta
     2041 tatgacagtg agaagagctg ctacagattt acaactttct atttctacta taagtaggct
     2101 tgtaaaagag tttttggatt tagggataat aagattagtg tctaagggtg taaaaggtaa
     2161 tcgttctgta tatagttatg taagtataga gtttaattga agtttatttg atgatagcaa
     2221 atatatggaa cttgactgtg tatgtaatga tgaatttatt gataatagtg gcatagagtt
     2281 tgattgtaat agcaaagata attcttttaa tggagatata aatgtagaaa ctgagcattg
     2341 cattaatgag tatttgtatg atgatgatgt agagcataat tataataatt tccacaacga
     2401 agatactata aagaataaag ccatttctaa tattagaact atcaaaaaat atattgatga
     2461 atcttttgat acaaataagt cagaaaattc taacttacaa caggagtcaa taaatttcaa
     2521 ctttaatgat acatgcaatg aaatagataa aagaacgaat aatagtatag aaaataatca
     2581 aaataaaagg tctatgagat tgagtgatat gaatgtttta agtgataaag gtaagacaaa
     2641 taaaaagaat tactttaatc ataatcatgg tatacaaaaa gggactaaat ctgtaacaaa
     2701 gaaaaaagaa tccttaaata aatttaataa aaaaaatata cagcaaagca ttataaatat
     2761 gctaaataaa aaatcaggta agaattttaa aatcgattct ccaataagca taaaacttat
     2821 caatgaaagg ctgaaggaag gctatgtctt agaagatttc tacaaagtta ttgaggtaaa
     2881 agtggctaag tggaaaaata ccataatgga aatgtatata agaccagaaa ctttgtttag
     2941 ctataagttt gaatcctatg taaatgaaaa catatctatg aaaagtcaaa gtaatgttca
     3001 cccgtgtaaa gaaagcaata gtaatgatag cagtaattat atacattctg attatacaac
     3061 atatgatgga agcgtgcgtg agagaaaaaa atatgaaaat gtaagatact ggaatcctat
     3121 atgtgaaatt taattagttt aagaatgtat ttatgataaa taagatatta atagagcata
     3181 gttagctata gcaaatgata tatcaataat tagtagcgaa ttatattgaa caatatatca
     3241 ataattggta gtaagttata ttaagtaaga tattactatc gagtagtgaa ttatattgaa
     3301 caatatatta ataatttgta gtaaagtata tcaaataaga tattgctatt atgtagtaaa
     3361 ttatataaaa caatgtatca gaataaatag tatattaata atttgtagta tatcatgacg
     3421 aataatatag tcataacaaa tagtgagtca taacaaataa tgtatttata attgataatg
     3481 tatttaaaaa tcaaaatata aaaacttatg aggaaagtga gattaattta tatgggtaaa
     3541 tcaaaagatg ttgaaataga gaagtctatt ttaggcacaa ttcttttaga caataaatta
     3601 agctataagc tagatgaatt aaatgaaaac atgtttatga atgatatatg tcttgagata
     3661 tttaaaatta tgaaagaact taaaaaagaa aacatagtga ttgatgtggc cactgtgaaa
     3721 tcaaagatag ataaaaaatc tctagcaata aaaacgagtg atgtgactaa tataattact
     3781 tggggtcaga attttgggct tgatggtcat attaaaattt taaaagaaaa tttagctaga
     3841 agaagcataa atcaaaactg ccaaaacctt ttacacagcc taaaccttgg agaaaatatc
     3901 gatacttgta tttacaaatt tgaaagtaat ataaaggaga ttttggacaa ggatacttat
     3961 gaaaatgatg atgtaaatag tatagcaggg aaggtgcttg attttttaga aaacaaaaaa
     4021 gatattggat ttaaatttgg tataaaatta cttgatacca ctataggagg tctttttaaa
     4081 ggagagctaa ccacaatagc agctaaatcg ggggttggga agactgcttt ggcacttcaa
     4141 ataatgttaa attcatttaa gcaaggtaaa aaaactttat ttataagtag agaaatgacc
     4201 agtgagcaag tatttatgag aaatatatgc agagtaactg gtgtaagtac aaggaatatg
     4261 aaatctaaag aaatagatga aaatgattgg aaacttattg taaatgcgat aggtgattta
     4321 agcgaaaata acttaattta tatcaatgat aagatagata caattagtgc aattagaaag
     4381 aggataaggc aggtaaagcc agatttatta atagtggatt atgtgcaact ccttacatct
     4441 cagaaaagca tggataagag agaaagggag gtagctactt tttcgagaga gcttaaaaat
     4501 atgactttag attttaatat acctgtaatt caattaagtc agttaaatga tgagatgaaa
     4561 gattcaagac cttatggaga tagacctatg agggatagca aagccatcta ccacgatagc
     4621 aacaatgtgg tttatataca tcaattaaag gggagtgatt atgaagaagc agtgagagat
     4681 attggagaaa gtgaggaggc agttagagca tcagaataca ggggaattaa gatggttgat
     4741 ttaatagttg cgaagtgtag agatggacag actagacata agcatttttg ttactttggg
     4801 gataagttac attttcaaga actaaattat taggtcaata aagtagtaat aacaagctta
     4861 ttaaaagtcg agaaatagca aatagattat aaaatatgag gtgaaaatat gttggaatta
     4921 acaaaaatta tagaattact tgaagaacac aagatagaag taagtattcc aaaggagtta
     4981 cataagtata gtttattgga ataccttgaa ttgtgtcaac aaagtggggg aacatatttt
     5041 tatattaaga cactggaagg ggctttaaaa gaggctagaa atgagcttat tattaaattg
     5101 tttaatggga aaaattatat ggaattagcg aggatgtttg aaatgagtgt tattaatgtc
     5161 aggcatattg ttagaaaaaa atagatattt ttggagaata caagtgtaat atacttctga
     5221 aatagaagtt attataattg aaataaaatt gtggttaata gattaaagtt gtgtataagt
     5281 ttaagattat agaggagtat aagtgtgatt aacagcataa ttttgtgaat aaaattgagg
     5341 atatatatga gctaaatgtg tgattaataa ggtaaggttg tgtataagat tatgagtatg
     5401 taaaaggata gtctataatt aatagtgcag agttgtgtgt ttggttaaga ttatataaga
     5461 atttaataga ttaaactata ctaataaggt aaaatcgtgt gtttagttaa aacttattat
     5521 aaaatcttga tacatgatta atgatgcaat atgtgaataa agttaagatt ataatagatt
     5581 taacctgtga ctaatagaat aaaattgtga ataaagttaa gattataata ggtttaaatt
     5641 gtgactaata gagtaagagt gtaaatagaa ataaacttca atatgggctt aacttgtgac
     5701 taacagagta gggtgtaaat agaaataaac ttcaatatgg gcttaacttg tgattaatag
     5761 agtagggtat aaatagaaat aaacttcaat atgagattaa cctgtgatta atagagtaag
     5821 gtgtaaatag aaataaactt caatatgaga ttaacttgtg actaatagag taaggtgtaa
     5881 atagaaataa acttcaatat gagattaacc tgtgattaat agagtaaggt gtaaatagaa
     5941 ataaacttca atatgagatt aacttgtgac taatagagta aggtgtaaat agaaataaat
     6001 ttcaatatga gattaacttg tgattaatag agtaaggtgt aactaaagat aaacttcaat
     6061 ataagtttaa cctgtaacta atagaataag attgtgaata aagttatagt tttagatggg
     6121 tattaacctg tgactaatag aatatgacta ggtgtaaaac tatagagttg tataagaaaa
     6181 tattatagat atattaaata ttagataata aatatttata tagatacaca gaagaaaagg
     6241 cgatatgaag ttaaacttaa tgacttcata tcgtttttta tttgaaattc ctttgtattt
     6301 agagtgtgac ttttaatatt gaggttttat gtgattaagt cacagataaa aagtaagtat
     6361 cttgatataa ttattataga ttaataatat agggaggtta ttatgtctaa taaaaaaaag
     6421 aagtatgcaa atattttagc aaatgagtgc gttgcttgtg gtagctgtat aaaagcatgt
     6481 ccaagaagtg ctatctcagt tccatgtggc attagtgcaa aaattaatag agatttatgt
     6541 gttggatgtg ggatttgtga aaagatatgt ccagcatctg taattgagat tataacaata
     6601 ttaaaagagg aagaaggtaa gtgtcatgaa taataaaaaa cattggtatg actatttatg
     6661 gattttttct tcaatatatt tgatattggg atttgtaaat attattttcg cttggattgg
     6721 gctaatttgt ttctttgttc cactagctat ttctattgtt aagggaaata aagcttactg
     6781 taataagtat tgtggaagag ggcaactgtt taatttgctt ggaaataaat tgaagctgtc
     6841 aagaaataga gatattccta aatttattag aagtaagtgg tttagatatg ggtttttgac
     6901 attttttatg gtaatgtttg ctaatatgct tttttcaact tatttagtat ttgaaggaag
     6961 tagaaatttt aaagaagtag tgactttgct gtggactata aaaatgcctt ggcaatgggc
     7021 gtatcatgga actttagttt caccttgggt ggtacagttt gcatttggtt tttatagtgt
     7081 tatgcttaca tcaactgttc ttggtttgat tactatgatg ttgtttaagc ctcgttcatg
     7141 gtgtgtttat tgccctatgg gtactatgac tcagatgata tgtaaggcaa agagtaagtc
     7201 tgtatagaaa ggctttatgt gatgtttaca gtgttgttca agtagagata agtagtaggt
     7261 ttgaatatat gtgacaaata tatgagtata ggggcataga tttagtttgt gtaaggtaaa
     7321 ctgatttatg cttctttttt atgtcttaga ttattggcta aatggaaaca atgaatttag
     7381 atattaagaa ttattcagtt atttgcaaaa aatataataa taattaacat tatatataag
     7441 ccaattaata gcaagattta tttgatataa aataggtcaa gcatttaaac taaataaggt
     7501 cataacgatt aatataagta tatatataaa aagattagaa gttataactt ataaaagata
     7561 ttctatataa atagtatggg aaaaatagtc aaactaactt attagaaaca aaaaatatac
     7621 ataaaaatga atgtaaatat caaataaggt acgatatcaa aatagatata agcatctttt
     7681 ttattattga aattatgaaa atacatttga tttcacacaa aaaagagtgc ctcatgaact
     7741 aaaaagttca ttttgagaca cacttttaca gtgtcaaaga acgaagttta tccttatcca
     7801 atacttttat accaccacga gataaagaca caataccttc tctagcaaaa tatttcaaca
     7861 ttctcgatac aacttctctg gcactaccca tgtacttagc aatttgctca tgagtcatat
     7921 ttatagtgct actgccattt ttagctatct catcaattaa aaaagatgcc aaacgcttat
     7981 caaaactcat aaaaagaatt tgttgcatag cccacataac atcagaaaaa cgttcggtag
     8041 ctaatttata tgaaaataac tctgcatgaa tattttctgt agataatttt gcaaaaacag
     8101 aggaactaat ctgtataata tcgcagtccg tttcagcatc tacatagaca tcaaaagtta
     8161 ttgtttttag aatacaggaa gctgataaaa tacatacatc tccatcatca agtcggaaga
     8221 gtgtaacctc tcgaccttca tctgaaagta tgtatgtacg tactgttcca gactttataa
     8281 tcaaaatacc aatacagtca ctatcaccac aatgtactgg attccctttt ttgtaatgtg
     8341 aggtatttgc tgaagaaatt aataattctt tttgtaagtc agtaagttta tcccaaaaag
     8401 gcaagctatg tgtaagtata tcaatttcat tttgtgaaat catacaatat ctcctttctt
     8461 aaattgttat aatcccatga tacaagtgaa tagataaatt gacaagtagt ttgttaagag
     8521 gattatttag tcagatagta atagtatgat ttatgtagaa aaaatatgat aatatatatt
     8581 cttgctatta atgagaaagt gatatatttt gatgatagta ggtttgtata actagtgcat
     8641 acagtttatt tttaaaagat taagttcctt atgtagcctt atagacataa aaccagcaat
     8701 tatagcagca atcaaatcgg ctattggttg tgcatataag ataccattta acccatagaa
     8761 tacaggaata atcaaaatta tagggataaa acagatacct tgtctacaca tacctaaaaa
     8821 tccaccttct tttgcttttc ctagtgcgag gaataatgta gaatacactg tatagaatcc
     8881 aaatagcata aatgaaaaac catttgccct aagagcttta ctaccatttt ctattaatag
     8941 aatgtcacca cttgtaaatt gtgaaattat agattttggg aaaagcacca atataagacc
     9001 aagcattaca caaaatattg tagtccataa tatggaggtt tttattgcta tatgtaatct
     9061 atcgtagttc tttgaaccaa aattataacc agcaattggt tggaaacctt ttataaaacc
     9121 aaatacaata aggctaccta aagatgttat tcgtgttaca gcacccattc cagctatcac
     9181 agaatcacca tactttgtag cctccatatt agttaatgta atggcaagac tagtcaataa
     9241 ttgaaatatt aaggtaggta tgccaatttt taggatttct gaaaaaattt cttttgaaaa
     9301 acaacattca ctaattctaa aactaaatat acttttcttg cttaaaatat atcctatata
     9361 aacaagtgtc gaagcaatct gtgagatagc tgttgcaata gaagcacctc ttacacccat
     9421 accaaaagta taaatgaata tcgggtctaa aatcatatta agcaaagcac ctaataacat
     9481 agcacacata gttgttttag cagctccttc acttgtgaca atattagtca tagtgacgtt
     9541 gaatatatta aaaatagatg atactacata aatgctagca taagatattg cataaggcag
     9601 gatacttttt gtagctccta acaattttaa aattggattg agaaaaatca taatcacaat
     9661 aataactaat gtaccaacta taatactact gtaaatagat gtactgacca ctttccctgc
     9721 tttttcatta tcaccatgac ctaaaagtct ggcaatgtat gaagcagctc catttccaaa
     9781 caataatcct aatcccacaa caagctgacc taatggaaat gcaacggata tagctcccat
     9841 ttgacttgtt ccaagtccac ccacaaagta tgcatcaact aaattgtata atgcagttat
     9901 catcatacct atcatggtag gtaatcccat agataacaat gctttcgaga ttggggcaga
     9961 ccctaatagt tcaattcttt tgttactttc tttcatagtt aacactccta ttctattgac
    10021 attaagtcta aattataata ctataaatta tagtaagtat ggaaaatagt actataattt
    10081 atagtatttg tcaatagtaa gaaaactaaa ggagatgatt tttatggcta caatagattt
    10141 aattgtattg ggaatagtaa agcaacaacc taagagtgct tatgatattc aaaaggcagt
    10201 ggaatacagg aatatatcac gttgggttaa aataagtaca ccatcaattt ataaaaaagt
    10261 aattcaatta gaacaaaagg gatatattga aagtaaaacg gtgaagaatg gaaaaatgcc
    10321 agaaaaagca gtttattcat taactgaatt aggtgagaca cattttttag atttaatgtt
    10381 tgatacatct agtagaccaa taaatattct tttagatttt aatgctgtta tagtaaattt
    10441 aaatagcctt acaccagaaa aacagaaatt atgtctcaat aatattgagg agaatgttca
    10501 agagttaaag aaaactattg aggaaaatat aagtataaag acagaagtac cagagacagc
    10561 aaaagcagtg ttggagcaac agttggtgtt agcacaagca ttggaaactt ggataaattc
    10621 tttaaaagat aatatgggaa attagaatta gagaagctaa cttaataatc attaaacaaa
    10681 aaacagcata tttaataatc agatattaga aaaaatgaaa caaatgatat aaactgaaaa
    10741 gtatatttaa aataaagctc acttaattga gagggaatgt taaaattttt aagaggaaga
    10801 gcaagagcaa tcagtaaagt ataaaattct attactataa taaaagatag aactagaaga
    10861 ctttatgaaa gtgagaaaag tatttatata aataaattta tagagtctat agtttaatag
    10921 taggaaatat cgtattttac ataaagattt agcaatatga cacactgttg tcgtgttctg
    10981 taggatatga ttaataaatc agatataaaa ggagagtgag taatatgcca gaattgctaa
    11041 aaaacatgta taatcgtgag tcactttatg aagttgcagt agctatccaa tcagtttaca
    11101 actcatttaa ggtagacgag ttcataaaat atacaatgga tgagacatgg aataacttag
    11161 aattaaaagc cagatgtcga aagataagta tgagcttagg aatgtattta ccagaagact
    11221 ataaggaagc tctttccatt cttgaaaaat cagttgcagg tttttatttt gcttttttct
    11281 ttccagattt tgttgaggtt tatgggcagg acgacgtaaa ttgggattta tctataagtg
    11341 cattagaaag aaatactgag tattggtctt cggaatttgc tgtaagagca tttatcatca
    11401 aggatgaaga gcgtatgatg gcacagatgc gtaaatggtc taagcataaa aatgaacatg
    11461 tacgaagact tgcaagtgaa ggctgtcgtc cccagttgcc atggggacaa gcaatagcca
    11521 aatttaaaaa agaccctaca cctgtactgc ctatattgga gcaacttaaa acagatacat
    11581 caacgtatgt acaaaagagt gttgctaata atctaaatga tatctcaaaa acacatccag
    11641 atttagttgt aagcattgca aaggactggt acggtaaaaa taaatctact aactggattg
    11701 taaagcatgg ttgtagaact ctgctaaaaa aaggaaatcg tgatgtatta gctctttttg
    11761 gatatgatga tgctacttca ataaatattc aggactttac tttagaaaca acgtctattt
    11821 ctattggaga ggatttaact ttttcattta atattttagc aaaaaaagcg acaaaaacaa
    11881 ggctcgagta cggaattgat tatgtgaaat ttaatggaaa aaggaatagg aagatattta
    11941 aaatatcgga ggtttcatta aaagaaaatg agaaaaagtc atatatgaaa aaacattcat
    12001 ttgcagatgt aagtgtaagg agacattatc caggcatcca ctcgattact attattatta
    12061 atggggtaga aaaaggtaaa cttgattttg agttaggtgt ataattttac taacagcata
    12121 acctatatca gatactttgc taaacctata tcatatattc tactaaatct acattaggta
    12181 gtttactact tgtttactga atttaaatac aaagagacac aggtgctaat ccctgccatt
    12241 aaaggtatat gagcataaca caaaagtaga acttatcaat tctgttacag gtacagtagt
    12301 aaatcctcag aatcacaatt agcaaatgaa gtgtaaaaaa taagtgactg tttattttgg
    12361 ttgatagtga tattggatag ttatacaata ccaatataaa ttagaatttg tagaggagaa
    12421 atataaatga atgtatatag tttttggaat gacactttgg cacaagatgc tgttaagata
    12481 agaaaatatt ttcatgaaga tgcttatatt aattggcatg atacaaatga gcaatttact
    12541 ttagaagaat ttattattgc aaattgtgag tatcctgata aatggaatgg taaagtagaa
    12601 agggtgctag aatttggaaa tttagtaatt acagtgacac atgtttatgc aataaataaa
    12661 ccactatcat ttcatgtcgt ttcatttatc agccttaaag atgataagat tatttctatt
    12721 gatgaatatt ggggagagga tgatgatgtg ccacagtgga gattagataa acatattgga
    12781 aagccaataa aatagcgttt gttctgtttt ataactgaaa ttttgataac atttgatatt
    12841 ttgatttgtc attggtcagc atagtcgtta gtgtaataat ttaaaaaagt agaatttgga
    12901 gagtgaacac tatggaagaa aaatacatct tatctgaccg agggaaagta tattattgga
    12961 taaaagaaag taatagtgtg tttgcaaaga acttaatttt tttgccagga ctaacagcaa
    13021 atcatcatct ttttgatttc caaatacagt acttttcaaa aaatcataat gtattggtat
    13081 gggacgctcc tgctcatgga aaatcaagac cttacttaga tttctcatat tctaacttgg
    13141 tagaagaatt gaaagtcatt ttagataaac aaaatatgaa aaaggttgta cttatagggc
    13201 aatcggcagg gggatttgtg gcacagtcct ttattgtaaa atatccaaat atggtagaag
    13261 gactaatgat gattggaact tgtccatatg gaattgacta ttatagtaag tcagatattt
    13321 tctggttaaa acagactgaa tggatgttaa agttattccc agataaattg ttgcgaaaca
    13381 taatatctaa gatgtgtgga gctacgtctg aaggcagaag gagtatgatg aaaatgttag
    13441 aaggctacga aaaaaaagaa ttgtgtcatc tcatgtatct gggatttgct gggttcatac
    13501 cagaaattca agacttaaat atacattgtc cagtttgttt ggtggttgga gataaagaca
    13561 agacgggtaa agtgagaaaa tataatgagc agtggcacaa taagactggg tatccattgc
    13621 acattataaa aaatgcttca cataatgtaa atgtcgataa acctaatgaa ctaaatcaga
    13681 ttatggatat atttattaag caattaaata tataaaaact acatactcat taggtaaatt
    13741 atgtagtata tttgtttaca gatagttata gaaataaata tgtaataaac tacttaacac
    13801 ttaaaaagtg gatttgtcca gagcataata caaagcatga tatatataaa tttttaagcg
    13861 aaatacatca aaagaaggat tagaattagt ggagacagaa cagctattag agattataaa
    13921 tagttttaaa ggaggggtac tacgaatata gaggaaggta aatcatacgt tattcaagga
    13981 ttataaatga ttagtttaaa agtactatcg gataggactc tgcatattta tttaaacctt
    14041 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
    14101 aagaaaatga ttgagagtat accagcaaaa caaattttgc aaaaagtgaa atttgacaat
    14161 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
    14221 atatattgtg atagccgtag cacaatatac aacatagaga attttgataa agttagatat
    14281 aaggaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
    14341 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
    14401 aagcaggcac tggatttgat ttcggaaaat cattttggag tgtctattga taccaaaagt
    14461 agtttggtgg ttagagatat accgattttg aagaaaatta aaaaaaataa ctctgctatt
    14521 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
    14581 aatccaagtt ctgtacgatt tgacgcagtg aaagaactta atgattctgg aattttttgt
    14641 ggcatattat taactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
    14701 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
    14761 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gaggtatatc accaaaattg
    14821 gtatttaaat atcagaaaac atatggttta aactatgtat gtactataca aaataaagat
    14881 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
    14941 gatattataa aagcatacaa gatgtcggag tcatatactc aaataaaatt tttttaagat
    15001 aggctttgga taagatataa tagtggtgat tgatgtgttg atattatagt tgattgctag
    15061 agctaatatt atagatgaat attaatattg atactctagg taattgctag agctaatgtt
    15121 atagatgaat attagtattg atattctagg taattgctag tgctgatatt ataatttatt
    15181 attagtatta atattctagg gaattatcac tatattttaa aaaagtaaaa atatatatca
    15241 aaatgaatta aataagtact gtaagaagtt tgctaaacta aggtttgaat aaagtgaggg
    15301 tggagaactt atgatagaaa taaaaactat agaaataaat aaaaaaggtc aaattgacct
    15361 tttaatgtta gccgacccat gtgaagaaat gattgataaa tatcttaata aaggaactat
    15421 gtacgcattg tatgataatg aagaacttac ttgtatagca gttgtaaatg aaatttccaa
    15481 agaaatatgt gaattaaaaa acattgctac atatgagcat tttcaaaata tgggttatgc
    15541 ctcaaagatg atttattatc ttttagatgt ttatagtaaa aaatattcat caatgattgt
    15601 tggtacagct gaaagtggag tttcctttta tgagaagttt ggatttgttt attcacataa
    15661 aattaaaaac ttttttgtag ataattatcc agaaccaatc tttgaaggag aattgcaatg
    15721 tgttgacatg ttgtatttat catataattt ttagatagga taatattttt aggtagatat
    15781 aattaattta ttcatataat tattggaatt ataatttata aattatgtat aataagagta
    15841 taacttattt agaagctgtt atattgtatt agattcaaat aagatatata ataacttgtt
    15901 tattttgaat tttggaggag taattatgga ttcaaataat aatactataa aatcaactgt
    15961 taaaaaaggt atttcttttg gttcttgttt agcaatgatt atttcttata ctgcatggaa
    16021 atctatccca tgggctattt ttcatggctt aatgagttgg atatatgtac tttattattg
    16081 ggttaaatat gcatagatta aaataataaa taagtatgta atattgtgaa gtttaagaaa
    16141 ccaaatgtaa gggagtgtct cgaagtgaac tttttgttta taaggcactc ttttttgtgt
    16201 taaataaact acattttcat tactgaaaca atgaaaaaga tgattatctc tattttaata
    16261 cataccctta tattcattca tttatcaaca atagttttaa aaaaaaagtt atctaaaatc
    16321 tattgtaatc taattgcttc tctctaaagt cttaacttta gtaatgatat atgagtttat
    16381 caggttctta tttcaataat gcaaattttg cttagaagtt cagactttgg tatgaagaaa
    16441 tgggtaataa taaaaaagag gtgttgatat gaaggaaaaa ttagaagaaa aatctaaaat
    16501 tattaaaaca tcaaaagagt ataagagaac tgtaattgaa gaaaaggaca gagaatggac
    16561 caaaaaatta aatgacatac ttagtagagg tgatttttct aatgaaaaat tagataaaat
    16621 caaggatttg attccaaaag aaatattgac tagtgaaaat gttggagaag ttgtgactga
    16681 agatgggtat gctaagcccg agtatgatga ggtatttaaa tcattgttta ctttaaataa
    16741 tgattatgat ttacttgcaa attttataaa tgatattctc aaggatgctc catatgctaa
    16801 taggaatatc aaaccgttca ctcaaataaa aagaataatt aaggttgaaa ccgacccaac
    16861 aattaattac attggtgaga aaagacctag acttgatatt ttggctgaag atgaagaacg
    16921 taaccatata aatattgaga tgcaaagagc tcttgagaac gattatttgg aaagagctga
    16981 atattatctt agtcgtgtac atggtagaaa gttagaagaa ggtaaagaat ataaagaaat
    17041 tggaaaaact gttggaatac atatactaaa tcatgtaaaa tataatcaca tagaagatta
    17101 tgtaaattgc ctgagattaa caatggatgg gcatccagat attttttcta gtaaaactgc
    17161 attatatttt atagaacttc caaaaattcg taaatccagt tgtatagcaa acagagttct
    17221 aatatggggt aaacttattg ataatccttc tcatatcgat attagaatac tatctaaaac
    17281 ggattatgtt attaaaagag cacttgatag attaaaagaa ttaggcagta atgaggacta
    17341 tttattaaat ttaaagagag gtgcgtatat tatgaataaa agtagaaatt ttaaagaaga
    17401 aatttttcaa gaaggtattg aaaaaggtat tgcaaaaggg aaaagagaag agaaatttaa
    17461 tatcatcata aaattaaaaa ataaaggata caatttgagt gaaatatgcg atattataga
    17521 tgatttgaat aaatcagagg ttgaaaaagt ctataatcaa aactaatact atgattaaga
    17581 tagcactaga taaatacaaa gcttcctctt ttaaatttaa attaatataa aactttgtta
    17641 tttaaatatg gtagatataa agagatatat aaataatagg tacaaagagg tgtataaata
    17701 aaagagataa ttcaaaataa aaatttgaat tatcttcttt ttatagaaaa agaaaggggt
    17761 tgatttatat atgaaatttt atactataca gactttagaa ttttggaata acaataaaaa
    17821 caataaatat ctatcaaatg attattctct tatagtagaa gattttgata ttccatataa
    17881 atggatgtat agtcaaatga tagaaagaat acctaaattt gatgatagta tgatatgggt
    17941 ttggtcgaag agacctgatt taaggtttag tgcatactta gagaggggtt taaaaggtgt
    18001 tttacttgaa atagaaataa gtgaagataa tgtgttatta tcagaatttg atttatggca
    18061 tttaccttta atggatattg ctgtagagat ttatgaagat gaacaaattg aaaagcaggt
    18121 aagttgggaa agaatatttg acttagaatt atgctcaaaa ttaaatgaag tgaatataga
    18181 agatttgaca ttgcaaggag tgacatctaa agtacctcta tctaatgtca gattaataaa
    18241 agaatttgtt gcaaaataat atttttattg aaatttattc aaaaaagtta tatatttata
    18301 acaagaggtg ttataaatat ataacttttt aagggggatt ttatatgtct aaacaacaaa
    18361 ctggtattaa atcaacactt atttcagaaa tattgggaat ttcttattgt attggagcta
    18421 cactagtaaa taagtatttt tttaaaggaa gtagtattga atatcttcct gtaattattt
    18481 gggtattagc tatgggagct caatgggtct ataataaaaa gtatgatgat ttaattgatg
    18541 aggcatcaaa atgtatacta tcaagagtta atgatattgc aataaaagta ttgttttttt
    18601 ctgtagctat agtatctata tttcttgtaa ctttggacgt aaatgtgtca aatttaaata
    18661 taggaatgtc tttattagtc attttgctta ttcagtcctt attgaagtta attctattta
    18721 tatattttga cagaaagggg atatacaatt aggtgtcagt attactaaag acaaaaatta
    18781 aatcatatag agaaaagaac cttatgacac aaggtgaact tgcaaagtca gtaggagtaa
    18841 ggcgtgaaac tattgtacat ttagaaaatg gtcgttacaa tccttcttta aaattggcaa
    18901 tggatattgc taaggtattt gatacaactg tagaagagtt gtttgaattt gttgaagaaa
    18961 ataatattta aatttcatgt attatttatg tattagagag tgaattaata gataaaaaat
    19021 tcaagtttat gtattagaga atgaattaat tataggcaaa aaaacaagct tatagataag
    19081 ataataaatg atttgtagat gaaaaaacaa gctaatggat taaagaataa atgatttata
    19141 gatgaagaag aaagcttata gattaaagaa tcaatgattt ataggtaaaa accaagctta
    19201 tagattaaaa aatgaatgat ttatagatga agaactaagc ttatagataa gagaataaat
    19261 gacttatagg taaaaaccaa gcttatagat taaagaatga atgatttata gatgagaaac
    19321 caagcttata gattaaagaa taaatgactt atagataaaa aaccaagctc atagattaaa
    19381 gaataaataa tttatagatg aaaaaccaag cttatagata agaagtccat gcttataagc
    19441 cagaaaatga atagtttaat agattaaaaa atacatattt gtgaattata gaatgaataa
    19501 ctccataaac tagaaaattt ataataatat ctatatgaaa attagaagta ttttatcaca
    19561 aaaaattaag ttaaataaat atgaaattac ttccaaatat ac
//
LOCUS       DAEMAE010000054        19210 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEMAE010000054 DAEMAE010000000
VERSION     DAEMAE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.055"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..771
                     /locus_tag="KPH93_001542"
     CDS             154..771
                     /locus_tag="KPH93_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG1688370.1"
                     /translation="MCKKRYLLVVSIFIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            879..3410
                     /locus_tag="KPH93_001543"
     CDS             879..3410
                     /locus_tag="KPH93_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1688371.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILVSL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDSIDGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTTVKDIYACGDVAQVGNTSLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3621..4241
                     /locus_tag="KPH93_001544"
     CDS             3621..4241
                     /locus_tag="KPH93_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG1688372.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDVKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIVSFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(4406..4942)
                     /locus_tag="KPH93_001545"
     CDS             complement(4406..4942)
                     /locus_tag="KPH93_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG1688373.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKVVLIIVYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5055..5729)
                     /locus_tag="KPH93_001546"
     CDS             complement(5055..5729)
                     /locus_tag="KPH93_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG1688374.1"
                     /translation="MSLLIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNSDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            5932..7806
                     /gene="asnB"
                     /locus_tag="KPH93_001547"
     CDS             5932..7806
                     /gene="asnB"
                     /locus_tag="KPH93_001547"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG1688375.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNVVVDSITNHMEEDKEIGTFLSGGIDSSIIA
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVVLSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSMLGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLRDAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETISNSNVDDFIDKKYVIKLLDTHLKGYRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(7898..9334)
                     /locus_tag="KPH93_001548"
     CDS             complement(7898..9334)
                     /locus_tag="KPH93_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG1688376.1"
                     /translation="MICTIKPLRISIKKSNEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNTTIPYIRLSNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNTDFPCKIKNDTLNWTKKIAFKNTLDLFSTSMPNCTPSIIKNFAVKLLCWI
                     EYFLPKLFYNKIKTNISPKVVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKAPDILEIPFERHITNINYNKNTNKTSPNTNKPNSENTLNNANLT
                     SKKIVQNEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDEVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            9663..10241
                     /locus_tag="KPH93_001549"
     CDS             9663..10241
                     /locus_tag="KPH93_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1688377.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            10280..10858
                     /locus_tag="KPH93_001550"
     CDS             10280..10858
                     /locus_tag="KPH93_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1688378.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLEAMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            10944..11573
                     /locus_tag="KPH93_001551"
     CDS             10944..11573
                     /locus_tag="KPH93_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1688379.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            11563..14322
                     /locus_tag="KPH93_001552"
     CDS             11563..14322
                     /locus_tag="KPH93_001552"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG1688380.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMVSKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWLKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTV"
     gene            14409..15971
                     /locus_tag="KPH93_001553"
     CDS             14409..15971
                     /locus_tag="KPH93_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG1688381.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKAKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKVSFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDRIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            15972..17108
                     /locus_tag="KPH93_001554"
     CDS             15972..17108
                     /locus_tag="KPH93_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG1688382.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            17172..18248
                     /locus_tag="KPH93_001555"
     CDS             17172..18248
                     /locus_tag="KPH93_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG1688383.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            18226..>19210
                     /locus_tag="KPH93_001556"
     CDS             18226..>19210
                     /locus_tag="KPH93_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBG1688384.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWISDENLSKVTRLEKELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMDSKESLKKEKILFLGTEEFMYIPMLFAKEKENQCDIY"
BASE COUNT         7587 a         1996 c         3393 g         6234 t
ORIGIN      
        1 attttgagac aatatcattt ttatataact ttaatcttag taagtatgaa gttttaaaat
       61 tactaaaata ggatgaaatt ttaatataaa agggtaaata aagggtttag agggcataat
      121 atagaataaa ttaattattt aaggagggtt tttatgtgta aaaaaagata tttattagtg
      181 gtaagtatat ttatagcggg tttattaact gcatgtagtg ggagtccaac tacggattca
      241 ggacaagcac aagataatac aaaaaaggag gcaaatgcat caaataacgc tttaaaagat
      301 gaaaaaaata atgaaaattt aatggaacaa gattttaaag tgccatacac agatgcaata
      361 aacatattta aagataaata taaggatgct gatatagtag atttaagtct tgagagagat
      421 ttaaataaat ttgtttatac tgtggaaggt gtagatgata ataatgaata taagatgaaa
      481 attgatgcta atacaaaaga tgtattagaa gataaaaccg aaaagttgga ttcagaagat
      541 ttaaatggag ttgctagaaa agaaaaatta gatttaaatg acataatgac acctcaacaa
      601 gctatggaaa ttgcattaaa agaacaaaat ggaatggtta aagagtggag tttagataaa
      661 gatttagatg ttacttttta taaaataaga atagataaag ataaaaatga atatgacata
      721 aaagtagatt ctaaaaaggg aactgtattg aaggtagaaa aagaagatta gaagatttaa
      781 actatcttta aaataatttc ttttttgaat aaattttgtt taaaatataa caaacagggt
      841 ataaagagac taaataacta ttttaggagg agtacggtat gtcattgaaa tcgctaaaaa
      901 taaaagaaaa tttatattgg gtaggttcgc ttgaccctga tttaagagta tttgacataa
      961 ttatgtatac accttatgga actacttata attcatatgt attaaaagga acagaaaaaa
     1021 cagttttatt tgagacagtt aaagataagc attttgacaa ttatattgaa agattaaatg
     1081 atttaaatat agattttgaa aaaatagact atattgttgt aagtcatact gaaccagacc
     1141 atgcaggaag tgttgaaaag cttttggatt tagctaaaaa tgcaaaagta gtagcttcag
     1201 aaacagcaat taaatatctt aaggaaatcg taaacaaaga ctttgaatat gttgcagtta
     1261 cagatggaga tacattatca attggggaca aaactttaga atttttctct gtacctatgc
     1321 ttcattggcc agataccata tacacatata taaaagagga taaaactctt gtaacatgtg
     1381 attcatttgg tagtcactat agtaatgata agatagtaaa tacacttgat gaaaatgaag
     1441 aaaaggatta cttggatgct ttgagatact attatgattg tataatgggg ccatttaaac
     1501 catctatggt aactgctata gaaaagataa aagatttaga tatagacact gtatgtccag
     1561 gtcatggtcc agtattaact gaaaatccta gaaaaattat agacctttat tacaactgga
     1621 gtgtaaatga acaaataaag ttagaaaaag aagttacaat ttgctatgtt tctgcacatg
     1681 gctatacaaa gattatggca gaagctataa aagcatatat tgaaaaaaat agtaactata
     1741 aagtaaattt atttgatgta atagagcata agcaagaaga tattttggca aaaatagcag
     1801 tatcacaagg tgtattattt ggaacaccaa caatattagg agatgcgtta aagcctatat
     1861 gggatatatt agtatcgtta aacccagttc ttcatggtgg aaaagttgct tcagtatttg
     1921 gttcatatgg atggagtgga gaaggtatag aaaatgctat ggagagaata agccagctta
     1981 gaatgacagc agtaaaacct tttgctgtta actttaaacc atcaaatgaa gagattgata
     2041 aattacattc ttatacaggt aaattcctag ataagttaaa ttctaccttt ggaagtaaaa
     2101 agaaaactaa gaaatttaaa tgtgtaattt gtaatgaagt ttttgaaggt gatagtgctc
     2161 ctagtgtatg tcctgtatgt ggagctaagg aagaccaatt tatagaagtt gaagaagatg
     2221 aagttacttt tagaaaagat actgatgaat atttcgtaat agtaggaaat ggagcagctg
     2281 gtttttatgc agcagatgct ataagaaaaa gaaataaaac ttgtaagata actatgattt
     2341 ctaatgaaga tgagttgact tactatagac ctgcactttc tgatggtata aatgaagaac
     2401 ttggttcaga tttctatatg gaagataaag attggtatga taaaaataat atagtagtaa
     2461 tattaggaac gaatgtagat aaattagatg aagtgaataa aactattata gtgaatgatg
     2521 gagctattaa atttgataag cttgtaatag ctacaggaag tagaaacttt ataccaccaa
     2581 ttaagggtca tgatttagaa aatgtgttta ctcttagaaa tataaaagat ttatatagtg
     2641 taaaagaagc actagaaaaa tctaagaaag ttgtagtgat tggtggaggt ttattgggac
     2701 ttgaagctgc atgggagttt agactaaaag gattagaagt agtagtagtt gaagcaatgg
     2761 atagcatact atcaaaacaa cttgataaag aaggtagtaa aatattagaa caatgtgtaa
     2821 gagatacagg aatagatgta agattgggtg tagcagttga tagtattgac ggtgatggaa
     2881 aagctcaaaa agttgtattt aaagatggag atagtgtcga ttgtgatatg gtagtatttt
     2941 caattggtgt aagagcaaac actcaaatgg ttcaggatac atctgtaaaa attgatagag
     3001 gtattgttgt agataagaca ttacagacaa ctgttaaaga tatatatgct tgtggagatg
     3061 ttgcacaagt gggaaatact agtttagcaa tatggccatc ttcagtagaa atgggaaaaa
     3121 tagcaggggc aaatgcaagt ggagataatt tgacatttga aagtgaagta tatcctgttt
     3181 cattagatgc aatgaatgta aaagtattta gtattggtaa tatacaaaat tttgataaag
     3241 aaatttcatc aaaagatgaa ggtcaaagaa tatataaaaa attatttatg aaagatggtt
     3301 cattggttgg agcaatactt ataaatgatt taagctgtac tgttaaattg ataagattaa
     3361 tatctgaaaa aggtgatttt gaggatatta tgaaggctga tatactataa tttaaatata
     3421 ctaaaacaaa aagtcttaaa atatgacaat tcatatttta agactttttt gttataatat
     3481 ataataaaat aattatacct taactgtaaa tttatataga ttatttactt tttattataa
     3541 ggttcatttt ttgagattag tatataataa acatatagag tatataataa tagttaaagt
     3601 taaaaaaggg gaaaagcaat atgcagtata tacaagaatt acttgatttg actcaaaatt
     3661 attgggtgtt agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag
     3721 ccttaccact tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat
     3781 gtttgttatc atggattggc tcaggtcttg ggacaacctc attattttta ttaataagta
     3841 gatttaatga tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa
     3901 gttggatgga taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc
     3961 ctggttgttt agttactatc gcatcagctt ttggtaaaaa agatgtaaaa gattttgtgc
     4021 cagcaatgtt ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata
     4081 tagaaggttt tataacaagt cctttaaaga tagtatcatt tatattattg gtgtttttgt
     4141 catggaagat tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc
     4201 ataaaaaaca tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt
     4261 atcttaaaat gatttagtac taaattattt taagatacgt tttatttttt gtattaagga
     4321 caaaattttt agctcttttg taaactaaaa tatataagac ttatttgtac ttagttcaaa
     4381 atattgttat ttattgtttc tacaactata ataaaatata aaattgatat ccaatcaaaa
     4441 aaataactat gaaatttaat agcttagata tgaatttttc tgtttttgag ttagttttta
     4501 taggtagtaa agaaatgttt atggtaatgg gataaaacaa ttcaatacct tctttagtaa
     4561 taaggtcaag acacaagtga gatagatatc ctattataaa cccactggac aaacatgaga
     4621 aaactatatt attgttagtg aagattgtca gagatttaaa aaaactataa ataagtaata
     4681 aggcaattag actgtgagta aatcctctat gtctaaacct gcttccaaat aatttgtata
     4741 ttaacataaa ttttttactt atgtaagaac ctctcatatc tatatctgga aataaagaac
     4801 ctaaatatgc aaaataaata taaactataa ttaatactac tttatatgca atatcgtatt
     4861 taactaggta cagattgttt ataaagggga gtatcaaaag tgcaaatata taacctcccc
     4921 tttcatgtgt ttcttttatc atataaaaat cctcctgaat cgtcactata aacattatat
     4981 tggtaaattt agactatctt ttattatatt atatagcaaa cataagtttt tttcaaaagg
     5041 attttaatat ttaattaatc ttgagatttt aaatttttgt aattttctaa agcaccattc
     5101 caatttacta cattaaacca gttgtcaatg tattcatttc ttctattttg atattttaaa
     5161 tagtaagcat gctcccaaac atcaagtcct attataggag ttaggttttt acttacaggg
     5221 ctatcctgat ttggagtagt cataatagat aatttcccat ctttagtagc tacaagccaa
     5281 gcccaaccag aaccaaagac atctaaagca gatttttgga actcttgctt aaatttttca
     5341 aaagaaccaa agtctctatc aatagcttct tttaaagatt cagaaggtat ggttttttct
     5401 ggtgtcatta tatcaaaaaa gaatttatga ttataagctc cacctgcatt atttcttaca
     5461 gttgtagcaa tatctttagg taaagaatct gaattttgca ataattcaca taaagaataa
     5521 ttataaagct caggatattt ttcaagagca gcatttaatt tatcaacata agcttgataa
     5581 tgcttatcat gatgcaattt cattgtttct ttatctatat aaggttcaag tgcatcatat
     5641 gcataaggta atggtttaac cttaaatttg ttgttttcag gtgtaaaagc aaacgaagtt
     5701 atgcactgtg agattataag taaagacata ataactggta ttaatatttt tttcttcata
     5761 aaaatcacct cctaatatag tatttctaga aataaaaaaa atatgaaata ttaacagata
     5821 atttataaga atttaatagc aatttcattt taaattgaat aagttttaaa gccaaagaca
     5881 aaactatgaa atatagaata agactaaagg acagaaagga attttgatta tatggatggt
     5941 aatatttcaa tttattataa aggtgatata tctaaaaaag aagatattaa atttaaaata
     6001 aaagaatctg taaataatac aagactttct agtagaatag agtataagga tgaggaaaaa
     6061 acttttgatt ttgaaagatt taatgtaatt gatgctattt atggaaataa accatttgaa
     6121 aaagatggaa aagttattgt ttttaatgga aatatatata actatcatga aataaaagaa
     6181 gaattaatta aaaaaggaca caattttact acaaatgatg atattgaagt cttattggca
     6241 tcttatatag aatttggtaa gaattgtgta cataagatta aaggaatgtt taactttata
     6301 atctatgata gagaaaatga aagtatattt ggagcaagag atttatttgg gataaaacca
     6361 ctttattata taaataagga aaatgcaata attttttcct cagagtataa gtttttactt
     6421 gaatatatga aaaatttaaa tataaatgaa agaagcttac aaagttattt ttcatttcaa
     6481 tatgtacttc ctgaagatac tatgatacaa ggaataaggc tgataccagc aggtcactat
     6541 tttagggtag aaaatggtat actatcttta aagagatata ataaacttga gtttagaagt
     6601 agtacaaaat ttttttatac taaaaatcac ttaggcaata gagatgttaa tgaggatgat
     6661 ataagaaatg tagtagtaga ttcgataaca aatcatatgg aagaggacaa agaaattgga
     6721 acatttctgt ctggtggaat agattcgtct attatagcca ctgtggcttc acaaattaat
     6781 ccaaatataa aatctttttc tgcaggcttt agtgtcaaag gatatagtga attagaagta
     6841 gcaaagaaaa cagctgataa gttaggtata gaaaatatcc agattaatat aacacaagat
     6901 gaatatataa aatcattacc gaatgtaatt tattctttgg atgaccctat agcagaccct
     6961 tcagaagtag gtctttattt cttaattaaa gaggcaggta aacatgttaa agtagtattg
     7021 tctggagaag gagcagatga attatttgga ggatataata tatataaaga gtatagtact
     7081 atgaaatcag ttgtcaattc acctacttat attaaaagta tgttaggaag agtttcagaa
     7141 cttatgccaa acataaaagg aagaaactat ctttatagag ctacaacacc attagaaaaa
     7201 agatatatag gaaatgcaaa agtttttgaa aatagcgaag taaaaagatt cttttttaag
     7261 tataaggaaa agaatatata tgaatacctg ttgtctaatt tgtatagaga tgcacaaaaa
     7321 aataattacg attatataag taaaatgcaa cacatagatg tgaatacatg gcttcaagga
     7381 gatatacttc aaaaaattag taaactttct acagctgagc aggttgaact tagagttcca
     7441 tttttatata aagatgtatt tgatgtagca aaaaatttaa gaatggagca aaaaataaat
     7501 aagaataata caaaagtttt acttagggat gcatttagag aaatagtccc agaacatgtt
     7561 gttcaaagaa aaaaattagg atttccaact ccaataagag tttggttaaa agattctcta
     7621 ggtgatattg taaaggaaac tatttctaat tcaaatgtag atgactttat agataaaaaa
     7681 tatgtaataa aacttttaga tacacatctt aaaggttata gagataattc gagaaaaata
     7741 tggactatat ttacattttg tctatggcat caattattta ttgagcataa aaatgtcgag
     7801 tattaatact gttttcaata ttaatcagag ggcaaaagca tgtaatgttt tcgccctctt
     7861 tattattatg gagtaggata agtagaattt tgatttatta gtttactatt tcaataaaat
     7921 tattggcaaa tattttgaca agactacttt caacagctaa atttaagttt tgtccatcat
     7981 catatccagc ggaaatcaat cctagtaagt ttccaaacat atctaaaaga gctcctcctg
     8041 aactaccact agagattgga gctgtaaatt gtatcatttg aaccgtttca aaatcacgga
     8101 aagctgagat aattccatct gaaactgtat taaaaagacc taaagggctt ccaattgcaa
     8161 ctattttttg acctctcaca ggttttttac tgactttaac aggtattggc ttgcattttc
     8221 tatcaacctt tatcacagct aaatcataat ctgaatggta ttttataatt tggtaagatg
     8281 tataaacttc atcatcattt tcaaatctaa ctaaaaatga ataaccatca ttgactacat
     8341 gaagattcgt aagtatataa ccttcattat ttattactac tccagaaccg ctcttaaacg
     8401 gtttattttc gttattgcat acaacaatca taacaacaga agctgataac tttgcaaggt
     8461 cttcataaga aagttctact tcctcatttt gtacaatttt tttagatgtt agattcgcgt
     8521 tattaagtgt attttcagag ttaggtttat ttgtattagg agaagtctta ttggtatttt
     8581 tattgtaatt tatatttgta atatgccttt caaaaggtat ttctaatata tctggagctt
     8641 tcctagaaag ttcaaataga gtagaaaatt ttgaacattc aggatataaa tcaagaatat
     8701 ctttatttgg atttatatat aaaatatcac acccaagttg tgataaaaaa tataagaaaa
     8761 aaagttcttg tcttttaata tttccaaaat atactacctt tggtgaaata ttagtcttta
     8821 tcttgttata aaataattta ggtagaaaat attctatcca acacaaaagt ttaactgcaa
     8881 agtttttgat gatagagggt gtacaatttg gcatacttgt ggaaaataaa tctaaagtat
     8941 ttttaaatgc aatcttttta gtccaattta aagtgtcatt ttttatttta catggaaagt
     9001 cggtattata taaatcccaa ctgttaaact tagaactatc ataaatatta aaaattttaa
     9061 gcatcttatc tgtttgttct ttgtcaaaat ttatttttag attgcttaat ctaatatagg
     9121 gaattgtagt attattaaaa aaataagtat ctatattttt aattttttct atgtatttat
     9181 caattgaatc atcaacacct atatatcttg taaagtagac ttctttaata tatgaagaac
     9241 tcttattttt ataggtagta tcagatataa attcaattag aggttcattt gactttttta
     9301 ttgaaatcct taatggttta atagtacaaa tcatattttt tctcctataa taagatgata
     9361 taatctatta taaaacaact taaaacaata tagtacaata attttgtagt ttcaaaataa
     9421 aatatgtact tgtttatata aatgatatat aattacatat taaacatagc ttttatccat
     9481 ttaataaagt atgtaaaatc tatgtaaaaa gtaagaaata taaacatttt tttaattata
     9541 ttttaaaaat atcaagcaag ttttcttcac aaatgaataa aaaatgttat aatatgatta
     9601 aagaaaaaga taaataatta gcaagatttg tttagctaaa attaacaagg gagcgataaa
     9661 ttatgggtat aacattatca aaaggccaaa aggttgattt aactaaagga aatccaggtc
     9721 ttaaaaatat tttagtaggt ttaggatggg atactaacaa gtatgatggt ggatttgact
     9781 ttgatttaga taccgcagca tttttaacag gagcaagtgg aagtgttact aatgatggag
     9841 attttgtgtt ttacaacaat ttaaaacata catctggagc agttgagcat ctaggagata
     9901 acagaactgg cgaaggtgat ggagatgatg aacaaatagt tgttgattta tctaagattc
     9961 ctggagaaat aagcaagatt tcttttactg ttacaattca tgatgcgaca gaaagaagac
    10021 aaaattttgg acaagtaagt aattcttata taagaatagt agataaagac acaaatgaag
    10081 agttaataaa gtatgaatta ggagaagatt ttagtataga aacagctata gtagtggctg
    10141 aaatatataa gcacaatgga gaatggaagt ttaatgcact aggttctggc tttgaaggtg
    10201 gtcttgctgc attatgtgga aactttggaa taaacttata atttcaacta taacaataaa
    10261 ctaaaggggg atattttcca tggctatagt attaaaaaaa ggacaaaaaa tcgatttaac
    10321 taaaggaaat ccaggtctta aaaatataaa acttggacta ggatgggata caaattcatt
    10381 tgatagtgga tatgattatg atttagatgt aagtgttttc atggttggag aatctcaaag
    10441 agttgaaaaa gatgaagatt ttatattcta caataactta aaacatccat ctggagcagt
    10501 tgaacactta ggtgataata gaactggtga aggtgatgga gatgatgagg aaatattagt
    10561 gaatttagag gcaatgccaa agcatataca aaagatagct gtaacagtta caatatatga
    10621 ggctgctgaa agaagacaaa attttggaca agtaagcaac tcatatataa gagtattaaa
    10681 ttctgaaaat gatgaagaaa tattaagata tgacttaggt gaagaattta gtatagaaac
    10741 tgcaattaca gtatgtgaga tatataagta taatggagaa tggaaattta gtgcagttgg
    10801 agcaggattt gaaggtggct tagaagcact ttgtagaaac tatggcttaa atgtttaatt
    10861 taagcaatat aatatattaa atataaataa cctatacaaa taatgtatag gttatattat
    10921 ataaaatttt gtggaggttt agtatgtcga ttaatttatc aaaaggtgat aaaatagatt
    10981 taaaaaaatc taatccagga ctgagtaaca tacttgtagg tctaggatgg gaccctgtac
    11041 aacaatctgg tggaggcttt tttaaatcgt tatttggtgg aggacaagct gatatagatt
    11101 gtgatgcatc tgtatttatg cttaatcaag aaggcaaatt aagtggaata aaagatttaa
    11161 tttattttgg gaatctaaaa agtgcctgta agtcagtttt gcatacagga gacaatctaa
    11221 ctggtgaggg tacaggtgat gatgaacaaa tcctagttaa tttggataaa gttccatcaa
    11281 atattcataa attattgttt gtagtaaata tatacaattg tgtagataga aaacaacact
    11341 ttggaatgat agaaaatgcc tatataagag ttgaagacca aggtaataag aaagagatag
    11401 caaaatataa tctatctgat aattattctg aaaaaactac tcttatagta ggagctatat
    11461 atagaaaaga tggtagttgg caatttaaag caattggtga aggaacaaaa gatgcgggtc
    11521 ttaaagaagt aatgcaaaat ttagacagga ttgagtgtgc atatggaatt taataatgat
    11581 atcaaatatt ataatgtaga taaagaagaa gttacaaaag acttatctgt taatcctgaa
    11641 aaaggtttat cagaatcaga agtcaagact agaagagaaa aatatggttt gaatgaattt
    11701 actccaaaag aagagggtag tttttgggat gatttaaagg aaagtttaag tgaaccaatg
    11761 atagttatct tgattattgc agcagtagta agtgcagtaa ttggggaaac tcatgatgca
    11821 atagggattg taggtgctat agcaataggt atagcaatag gtatgattac agaaggtcgt
    11881 tctaagaaag cggcagaggc tctttctaag ttgactgaaa acatagaagt aaaagtattg
    11941 agagatgggg aagtacatca aatttctaag agtgaattgg taccaggtga tatagtatat
    12001 attgaaactg gtgacatggt acctgctgat ggtagactta tagaaagtat aaaccttaaa
    12061 attagagaag atatgttaac aggagaatct gatgatgtat ctaaaaaatg tgatgtagta
    12121 gtttctatgg aagagataga atctaaagga gcagtagtag tacaggagcc aatacctgca
    12181 aaacagataa atatggtgtt tggtggtaca cttgtagctt atggtagagg agctctagtt
    12241 gtaacttcaa ttggagataa tagtgaaatg ggtaaaattg ctcaaaactt atcagaaaca
    12301 gatgagcaaa ctccacttca gataaaactt ggaaatttag gtggtttgat agccaaagtt
    12361 tctagtgcag tagcaggatt attatttata tttatggttt tccaaatggt aagtaagaat
    12421 gtattaaatg tagatatgtc tggaatatta ccgtttttag aatcaataga cccagttaaa
    12481 acagcattta cagtatgtgt tgcgttgata gttgcagcag taccagaagg tcttccaact
    12541 atgataaata tgacacttgc aataacaatg caaaaaatgg ctaaaataaa tgcattagtt
    12601 actaaaaaag aagcttgtga aacgataggt tctgtttctg taatatgttc agataaaact
    12661 ggtacattaa ctgaaaatag aatgactgta gaagttgcat atgttgatgg cagatatatt
    12721 gaaagttcag aagaggaaat aaatagttat tttgaagaaa actgtatgat aaactctaca
    12781 gctgatgtag agcacaatga tggtgatatt aagtatttag gaagtgctac tgaatgtgcg
    12841 ttgcttttat attacaaaaa cgtagattat agacaagtta gaaaaaattc taatatagta
    12901 gctcaaaatc cttttacttc agattcaaag agaatgactt ctgttatagg tcaagataat
    12961 catcatgtac tattatcaaa aggagcacca gaagtcttac ttgaattatg ttcacatatt
    13021 caaagaggaa aagacatagt tccattaact gaaaatataa aacatgagat tttagaagaa
    13081 ataaagaaat tgcaaataaa atcaatgaga actttagggt ttgcatataa agaaatttca
    13141 caagctgaag aagaagcagc agtaactgcc gaaagtgatg ctatgaatgt aagtgcaatg
    13201 gaaaacaatt tagtatttag tggttttgtt ggtataagag acccacttag aaaagatgtt
    13261 atagagtctg taaatacagc aaataaagct ggtgtttctg taaaaatgct tacaggagat
    13321 aatataaata cagcaagagc tataggcgaa gagttaggtc tattaaagaa taatatgaga
    13381 gtggttgaag cttcatatat agatacttta gatgatgaag aattaaaaca agagatacaa
    13441 agtatttcaa tcgtggcaag aagtaaacca gatagtaaaa tgagaatagt ttcagctctg
    13501 caaaagagcg gagacgttgt tgcagtaaca ggtgatggaa taaatgatgc tccagcactt
    13561 tcaaaagcag atgttggtat agcaatgggt atatctggta cagaggtatc taaaaacgca
    13621 gcagacataa ttttaactga tgatagtttt agtaccattg taaaaggtat taaatggggt
    13681 agaggaattt atgataactt ccaaagattc gttcaattcc aattaactgt aaatgtaatt
    13741 gcatttttag tggctataat atctcaagtt atgggacaag agatgccatt tactacaata
    13801 caattattat gggtaaacat aatcatggat ggaccaccag cactagcatt aggtctagag
    13861 ccagttaggg accatgtgtt aaatagaaaa cctgttgata gaaaagccaa tataatttct
    13921 aagtcaatgg tgtatacaat agtattaaat gcattttata taacagcaat actaatgtta
    13981 caatctacat ttaatttctt aggagttgat gaagctcata agggaacagt aatgtttgca
    14041 ttatttgctt ttagtgcact gtttaatgca tttaactgta gagagtttaa tgcagatagt
    14101 atcttcccta attttacaca taacaaatta gcactacaaa taatagtgtt gacaggaatt
    14161 gcacaagtaa tatttactca gctattccaa gatttcttta actctgtagc aatggatttt
    14221 atgacatggt taaaagtttt atgtgtagca gcaagtgtaa ttgtggttaa tgaagttgta
    14281 aaatgtataa taagactggt aaaaccaaaa aagactgttt aaaacaaatt ttttagaaga
    14341 acacagactg actcaaaata tgaattttgg gtcagttctg aataaacatt taaattggga
    14401 ggatagttat gggtgactca aaggtatttg aaaaaatatt ctccagccaa tcagatagag
    14461 gaaattacac tccttcaaag ggatatctaa gttattttat ttcgtatatt ggtttagaag
    14521 atgaagtttt gtacaatctc gagatattta aggcaaaaca aaatattgat agcaaaaaag
    14581 atattgcttt atttactgat gttatagcaa atcctagtga ttttgatatt ataaattatt
    14641 ttaaaagtgg acttcaaaaa tatagaacta gcatggaaga tgtagatata aatattttag
    14701 gatttgaaga gattgattat aaaataaagc aagcaatgga tagagtgtta aaagaagaag
    14761 aaaaagaatt tactaatgat agagttaaac aaaattttat agtgaaaata atggcatgga
    14821 taaaaattta tatcggtgct ttagatataa ataaaaatga agctccaaaa gtaatatttt
    14881 atggagatat aaaaaaacat gaagtttatt tactcttaat attatacttg gcaggttttg
    14941 atgttttata tcttaatcca aattcaaaga gtaatataga tattttaaaa tcagaaagat
    15001 acaatataga gtttgaagaa gccaacataa tagaagaaaa agtttctttt gaagaaaggg
    15061 ttattttagg ggaaaaaata gataaaagtt ctgtaaaaaa agcttttact gttggagcag
    15121 aagcatcaaa gcgtataagt gaggaattgt taaatgatgc aggatttata aaaccttggc
    15181 aactccaaga tagaaaaata aaaaatctac ttctaagttc aactgttgat gaaatatcaa
    15241 tatactggaa tcagccactt aagttaagac caggatttaa gtttaatgat gctattgtag
    15301 aagctccaaa ttttctttct aagattaacg gaatatataa tgataagaat gaatatatta
    15361 agtttttaga tttattaaga gattcggaaa gttcaacatt tattgaattt aatggagatg
    15421 tagatagatt ttcaaaagct tttacaagag aagcttttag tttatccttt ttattggatt
    15481 caaaaggtgt aatagataaa aactctgttt taaataataa agattattct atatcaacac
    15541 tagcattgaa tcaacaaatt atgattttag aaaaagtgga agagttgtta gaaggaagta
    15601 tgtttttgaa tggtttaagt ggagaagaca ggataaaagg tctatttaca gtacttcaca
    15661 tggataagaa atttgttcat atgatgaaca attttgatta ctcacttata aaccccaaat
    15721 tgattatata catgtacaag agtattgtat ttgataaaga aatagttttt cttatgcttt
    15781 tacttagtaa gattggattt gatattatca tcttatgtcc aggaggagaa aacaatatag
    15841 agaatgttat aaataaccaa ttgattgatg tacacagatt ggataaaatg gtatatgacc
    15901 ttaaattaaa ttcgcttgaa aatgatatac ctttacttaa aaaaattttt ggaaagagga
    15961 gacgatttta gatggggata aatataaata gtttttcaaa agataagaac tctgtagaag
    16021 ttactgagaa tttgcctaca gcagttcaag aagaaaactt cgatataatg gaatatacaa
    16081 ataataaaaa aaatgaactg agaaagtcta aggaagttga ggcattgaca tctcttatag
    16141 aagtagaaaa tccagatacg attttacagt ttggtaggaa agcatctgaa ggagtagcaa
    16201 gagtttctga tagcctttta aatacaataa aattaaatag aaatgaagaa aattctaaaa
    16261 tgctggttca tttaactaaa ataatggata agtttgattt agacgatttt caagaaacta
    16321 aggaaccaaa tttcgttcaa aaacttttca aaaaagctaa taatgccatt gaaatgatgt
    16381 ttcaaaagta tgaaacatta ggaggagaag ttgagaaaat tcaaattgag cttgagcaat
    16441 acgaaagaga tatagcactt tctaataagc aaataggtgc tatgttaaat gaaaactttg
    16501 agttttataa tgaacttcaa aaatatattg ttgctggaga aatggcaata gaagaaatgg
    16561 ataatgaaat actacctttc tttaaacaaa aatcagagac tagtggagac caaatggatg
    16621 ttgtcaatta tcaagaacta ttaaaagttt atgatatgtt aaatcaaaga gtctatgatt
    16681 taaggatagc agaaaatata gctatccaaa caatacctat gcttagaggt atgcaacata
    16741 ataattatgg tttaattaga aaaataaata gtgcatttgt cgtaacttta ccagtattta
    16801 aacaatgttt atctcaagct atattactta aaaagcaaga attacaagca aaatctctaa
    16861 aagcacttga tgacaaaact aatgaattat tgcttagaaa tgcacaaaat gtaagtactc
    16921 aaagtgcaca aatagctaga atggctggaa caagttctgt tcaaatagaa acattggaaa
    16981 aaatgtacaa tactatcaag tctggtatag atgaaactat gagaatagaa gaaaacaata
    17041 gagctttaat taaggataat acaaagagat tggaagaatt aaatacgact ataatttata
    17101 ataagtagta ttatattaag ggctaatttg tataaagttg gccctttcta tgtatatgag
    17161 gaggctagac attgaaatat ttcgattata tctgcaaaga tgatttggag agaatttttt
    17221 tgaaagagcc agaagacttt agtgctaaga ctgaaaaaga tgtgttgaaa tatgcactgg
    17281 gcgctttttt atacgttcca gctactcaat ataatatgat ttataagagt attatagggg
    17341 atgttaaggg agttagacca ttagctattt gtttagaaga tgctgttgga gtaaatggag
    17401 agttagaggc tatagaaaat cttagactaa ttttaaaaaa tattagcaat gaaagtataa
    17461 ctaataaaga tggaatacca ctaatttttg taagaataaa agatgtggag caactgttga
    17521 gaattaaaga gattataatt aaaaatagtc attctataac aggaatatta attcctaaag
    17581 caaattcgga gttaattgaa aattgtatag aagctttaga ttctatgaat ctccaagata
    17641 tgtatgttat tccgataata gagacaaaag aatttatata taatgaaaaa aaagaactta
    17701 gttttactaa tttgtataat gctattttaa gacataagtc aagaatatta agcattagag
    17761 taggcataac tgatatatta gggatgtatg gtataaggag agataagaac ttttctatat
    17821 ataataattt gatttgttct tcatttatat tagatataat cacatatctt caaagaccag
    17881 agttagatat acctataagt ggtggagtat cagaattttt tgatatgaca aataaggata
    17941 taagaaataa gtatatagaa gaaatattat tggataaata tcatggatta attggaaaga
    18001 ccgtaattca tccaatgcaa attcaaattg tacaagcgct atctacagta tcatatgaag
    18061 attttactga tgctttggat atactggata gtacagacag taagtatggt gtgagcaagg
    18121 gtgtattagg agagagaatg aatgaaacaa atccacattt tttatgggct aaaaaaacat
    18181 tgattctatc taaaatatat ggtgtattaa ataaaggagt agactatgaa gaattgctta
    18241 aattttaatt gtgagattga cattattgat aatccattga atttagaact aaatgatttt
    18301 ttagatgtag gaataagaaa taatagcaaa agacgttttt tatttatctc aaagaaactc
    18361 ggtaaacatt tagcatgtaa gccaagtgag atggataatt tgggaaaatt aatggctaca
    18421 atttataatg aaaaaaataa ggaagaatca aatggtactg ttatagcttt tgctgaaact
    18481 ggtacagctg ttgggcatag tttttttgat tacttatgtg gagattatga atttattcat
    18541 actactagag agcagtttga cgagttagaa aatctggaat ttttagagga acattcccat
    18601 gcaacagacc agaatttata ctttgggatg cttaacaact ttaaatttgg agatgagata
    18661 atattggttg atgatgagat taccacagcc aatacatgca tgaatataat taggaagatt
    18721 caaagcattt atccgaaaaa gaaatacact atatgctcaa tactcaattg gataagtgat
    18781 gaaaatttaa gtaaagttac tcgtttagaa aaagagctag attgtaagat agattttgta
    18841 tacttattta aaggaaactt taaattttat atagatgaag atattatttc aaattacaac
    18901 gacaattata gtattactaa attagagact atttcaagtg ataaagtaaa tttagtagta
    18961 aatcatattt atgttgattt agaagattat attggaaata agaaatatgt aaaatataca
    19021 ggaagatttg gaattaatag aagagaacaa tttagattaa aagaaatcgt taagagagaa
    19081 agtaaaaaaa taactatgga cagtaaagaa tctttaaaaa aagaaaagat tctattttta
    19141 ggtactgaag aatttatgta tatacctatg ttatttgcaa aagaaaaaga aaatcaatgt
    19201 gatatatact
//
LOCUS       DAEMAE010000055        19166 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEMAE010000055 DAEMAE010000000
VERSION     DAEMAE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.056"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..206
                     /locus_tag="KPH93_001557"
     CDS             <1..206
                     /locus_tag="KPH93_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG1688385.1"
                     /translation="KFIFLLPNIVLIKVLGSVIVVFVFIILFKKHIYKKIDGVTGDIL
                     GCGIELSELVYLIYIYLLIFMFF"
     gene            226..840
                     /locus_tag="KPH93_001558"
     CDS             226..840
                     /locus_tag="KPH93_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG1688386.1"
                     /translation="MIRLILIRHALTNDNKKGKLSGHIDSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTIEKISKLKLLEIEEKETLREISFGDFEGITFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      907..1086
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1216..2715
                     /locus_tag="KPH93_001559"
     CDS             1216..2715
                     /locus_tag="KPH93_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG1688387.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFRKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGRK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLNKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            2754..4130
                     /locus_tag="KPH93_001560"
     CDS             2754..4130
                     /locus_tag="KPH93_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG1688388.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDTSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            4131..5102
                     /locus_tag="KPH93_001561"
     CDS             4131..5102
                     /locus_tag="KPH93_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG1688389.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLITYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            5134..6204
                     /locus_tag="KPH93_001562"
     CDS             5134..6204
                     /locus_tag="KPH93_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1688390.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKSSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRTYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            6206..7093
                     /locus_tag="KPH93_001563"
     CDS             6206..7093
                     /locus_tag="KPH93_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG1688391.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNRLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVIGILTDKFMNDKKMIDALCESNINSVYNK
                     IYTLNIINGGIKGEIEWNT"
     gene            7081..7713
                     /locus_tag="KPH93_001564"
     CDS             7081..7713
                     /locus_tag="KPH93_001564"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG1688392.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETAKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            7722..8948
                     /locus_tag="KPH93_001565"
     CDS             7722..8948
                     /locus_tag="KPH93_001565"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG1688393.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVSKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINSSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            8945..9553
                     /locus_tag="KPH93_001566"
     CDS             8945..9553
                     /locus_tag="KPH93_001566"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG1688394.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDFKIGPKQISKEIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            9543..10118
                     /locus_tag="KPH93_001567"
     CDS             9543..10118
                     /locus_tag="KPH93_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG1688395.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            10111..10863
                     /locus_tag="KPH93_001568"
     CDS             10111..10863
                     /locus_tag="KPH93_001568"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG1688396.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            10847..11968
                     /gene="cbiG"
                     /locus_tag="KPH93_001569"
     CDS             10847..11968
                     /gene="cbiG"
                     /locus_tag="KPH93_001569"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG1688397.1"
                     /translation="MNIEGNIAIICITENGKKLAVKINSLVNNCIIYQVKNKESNLAV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHMDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKGELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKLEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            11965..12690
                     /gene="cobJ"
                     /locus_tag="KPH93_001570"
     CDS             11965..12690
                     /gene="cobJ"
                     /locus_tag="KPH93_001570"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG1688398.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTLVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            12687..13439
                     /gene="cobK"
                     /locus_tag="KPH93_001571"
     CDS             12687..13439
                     /gene="cobK"
                     /locus_tag="KPH93_001571"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG1688399.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRKLAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIIAMKGPFSQHINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYETVFNDINKIKDILV"
     gene            13458..14936
                     /locus_tag="KPH93_001572"
     CDS             13458..14936
                     /locus_tag="KPH93_001572"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG1688400.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDTCEDKIKNSLKSYDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNRDNKIK"
     gene            14966..15871
                     /gene="hemC"
                     /locus_tag="KPH93_001573"
     CDS             14966..15871
                     /gene="hemC"
                     /locus_tag="KPH93_001573"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG1688401.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            15861..17360
                     /gene="cobA"
                     /locus_tag="KPH93_001574"
     CDS             15861..17360
                     /gene="cobA"
                     /locus_tag="KPH93_001574"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG1688402.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYKT
                     AIKEEVKPPTLIVVGSVVGLREKLNFFERKSLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKIEKIQNNIKLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINRLGIKLYKESATSTIDSLVDLIIKN"
     gene            17377..18342
                     /gene="hemB"
                     /locus_tag="KPH93_001575"
     CDS             17377..18342
                     /gene="hemB"
                     /locus_tag="KPH93_001575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG1688403.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEEEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            18495..19073
                     /locus_tag="KPH93_001576"
     CDS             18495..19073
                     /locus_tag="KPH93_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG1688404.1"
                     /translation="MLYPINLKLDELDVVIIGGGEIAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEEIYTEDYEEYLNVLGDIRK
                     EVIKKYEDKSKRKDLLNMLITLDLEELKKFDI"
BASE COUNT         7912 a         1832 c         3633 g         5789 t
ORIGIN      
        1 ctaaatttat attcttatta cctaatatag tgttgattaa agtattaggt tcggttatag
       61 tagtttttgt atttataata ttattcaaaa aacatattta taaaaaaatt gatggagtaa
      121 caggagatat attaggctgt ggaatagaac tttcagagct tgtatattta atttacatat
      181 atttgttaat ttttatgttt ttttaaagta aaaagtggtg ataatatgat aagattaata
      241 ttaataagac atgcactgac aaatgataat aaaaaaggaa aattatcagg ccatatagat
      301 agttgtatta gtgaagaggg caaactacaa ataaataaaa ttactagata tctgagtaat
      361 gaaaacatag ataaggttta tacaacacca tctacacgta ctaaagacac aatagaaaaa
      421 atatcaaaat tgaaattatt agaaatagaa gaaaaagaaa ctttaagaga aattagtttt
      481 ggagactttg aaggaataac ttttgaagaa attaaaatca aatatcccaa tgagttcgaa
      541 aaaatgataa aagaaggtaa caactataga tatccaaatg gagaaagttt gatagactct
      601 tataaaagag tggcaaaaga aattgataat ataattttag aaaatgatag taatttagat
      661 acaaagacaa tacttatatg ttctcatgca ggaacaataa gaaatataat aacacatttg
      721 atttctggta gttataagta tcactggaac tttaaaattg acaatgcatc tattacagtt
      781 ttagaaattg atggtggatt tgctgtaata gataaaatga attttacaga cttcatataa
      841 tttaatcaaa accaaacatt acaatctgtg tttgtaatat atctgtaaaa ttagcaatga
      901 aaatagaata ttagattaaa attaggttct taattagatg ttaaaaggga aaaaggttag
      961 atgcctttgc agcccccgct actgtgaaac caacgaaact ataaatacca ctgtcagttt
     1021 gatgggaagg ttatagaagt aggatgaagt taagccagga tacctgccta atttaattta
     1081 aaacaagagg tcttcggggt gaaagatttt taattaatat atataaaagt cctatcttta
     1141 gaagtattag gacttttttt aatttgtttt ttagtaaatt ttaacaagga gggatggaaa
     1201 gtggggaaaa aaataatgct acaaggaaca gcttcaaatg taggtaaaag tgtcttagta
     1261 gcaggtttgt gtagaatatt taagcaagat ggttatactg ttgctccttt taagtcacaa
     1321 aatatggctt taaattcttt tataactaaa gaaggattag aaatgggaag agcacaggta
     1381 tttcaagcag aggctgcgaa aatagagcca atagctgata tgaatccagt tttattaaaa
     1441 ccttctggaa ataataaaag tcaagttatt gtaagaggta aagttgtagg agaaatgcct
     1501 tcctctaagt atcatgatta taaactggag ttagtagatg tgttgaagga aacatttaat
     1561 agcttaaatt caaggtatga tattgtggtt atggaaggtg caggaagtaa tgcagagata
     1621 aatttaaaag atagagatat ctctaatatg ggtatggcag aaatagctga tgcacctgta
     1681 ataatagtcg gagatattga tagaggaggc gtatttgcct cattagcagg aacaatgctt
     1741 ttattaaatg aagatgagaa aagaagagta aaaggtgtaa taatcaataa attcagagga
     1801 aaaaaagagc ttttacaaga tggtgtaaag atgctagaag acatcataaa agtacctgtt
     1861 ataggagtag taccttatac agatataaaa atagaagatg aagacagtgt aactacgaga
     1921 tttaggaaaa aaatgaataa aagtgatata cacatagaaa taattagaac cccacatatg
     1981 tcaaatttta cagattttaa tatatttgag acacaagaag atgtaagtat tagatatgta
     2041 gactatggag aatctttggg aaatccagat atattaataa tacctggaac taaaagtact
     2101 atagacgatt taacatatat aagaaaaagt ggtcttgaat cgcaaatcaa aaatttacat
     2161 agtcaaggta aattgatatt tgggatatgt ggtggatacc aaatgttagg tagaaaatta
     2221 aaagaccctt atcatgttga aagtgaaatt gaagaagttg atggaatagg tcttttagat
     2281 acagaaacaa cgtttgagaa agaaaagact acaacacaag tagaagctac tattgttgaa
     2341 tgtatcagtg aatatatgaa taaccttaag ggaaaaaaag taaaaggtta tgaaattcat
     2401 atgggcatta caaagcgagg taatgatgta agaagtttag atttaataac taaaaaactt
     2461 aataaagaag tgaattatac tgaaggaagt gtaaataaag aggggaatgt aattgggact
     2521 tatcttcatg gaatatttga tgaaataagc tttacaagag caattttgaa caatataaga
     2581 aagaaaaaag gtttaggaga attagaaagc aaagtaaact cttttaatga atttaaggat
     2641 aaagagtatg ataagttggc tgatttttta agagaacatt tagatataga gaaaatatat
     2701 gagattatga aataatgatg acctttattt ataatttagg taggaggtgt attatgaaaa
     2761 aaattttaat tgcgggtact aatagtggag taggtaaaac aacaatatca ttaggaataa
     2821 tgcaagcttt ggttaagaga aatatgaaag tacagccata taaagtaggg ccagactata
     2881 tagacccttc atatcacact tttataactg gaagacattc cagaaattta gattcttata
     2941 tgttagatga tgaaaaaata aaatatatag taaataagtc ctcaaaagac gcagatattt
     3001 cagtaataga aggtgttatg ggcttatatg atggttttgg aatagattta gataattgta
     3061 caagttcaca tacttcaaag gtattaaagt cacctgttat attagttata aatggtaaat
     3121 ctatggcagc atctagtgct gctatggtac ttggatataa ggaattagat aaagatgtaa
     3181 atataaaagg tgttatagta aataatgtaa agactaatag ccattatcaa ataattaaga
     3241 gttctataga aaaatattgt aatgttgaag tattggggta ttttcctcca aataacaaat
     3301 tctctttaga atctcgacat ctaggattag ttcctagtgt agaaatgaaa tcactaaggg
     3361 aaaaatttta taccttagca gatgaaattg aacactatat agatattgac agaataattg
     3421 aaatatcaga aagtgatgaa tttgagagtg attttgattt tcaaactttg atagaaaata
     3481 ataaaataaa agaacatgtt aataataaat caatagctat agcatatgat aaagcattca
     3541 acttttatta tagagaaaat atagagcttt tagaggaact tggtttagaa ataaactatt
     3601 ttagccctct tgaagataca tcagtgccaa atgcagatta tatttatatt ggaggaggtt
     3661 ttccagaaat atttggaaag gaattagaag caaatgagtc aatgagaact tccatattag
     3721 aagcacataa caataatata cctatttatg cagaatgtgg aggactgatg tacctaggtg
     3781 agaaactatt gaatcaagaa gaacaagaat ttaatatggt tggaatattt agtggaatta
     3841 gtaagatgac atcttcttta aagagatttg ggtactgtat tggtgtagcc aaggaaaata
     3901 ctcttttatc taaaaaaggg gaatctatta aaggtcatga gtttcatcat tcaatatttg
     3961 aaagtgatga aaaatgtgta tattcaatga gaaaagaaag agatggaaat atagtagaag
     4021 agtgggatgg tggttatagt aaaggaaaca ctttggctac atatcttcat actcattttt
     4081 ataataactt aaattgtata gaaaattttt tgaaaaaagg aaatgagtaa atgagtattt
     4141 tatctattta tataggatat gtgacagact taattgtagg agacccatat tcttttcctc
     4201 atccagtgag atttattggt aaacttataa attttactca aggtataata agaaaggtct
     4261 ctaaaaatga taggcagtta aaactggggg gatttatatt atggtttata actgttggaa
     4321 taacatattt aataacttat gctatagtga agttatttag ttttaatata ttattgagta
     4381 ttgtagtgaa tagttttata atttatacaa cactagccac aaaatgttta aaagacgaag
     4441 cattaaaaat ctataatgta ttaaaaactg aagatataga aaaatctagg attcagctat
     4501 cttatatagt aggtagggat actactaatt taagtgaacc tgaaataata agagcaactg
     4561 tagaaactgt tgcagaaaat actgtagatg gtataattgc gcctatgttt tatgcattta
     4621 ttggaggagc accacttgct atggcctata aagctataaa tacattggac tcaaccgttg
     4681 gatataaaaa taaaaagtat aaagatattg gttttgcatc tgctaagatt gatgatatag
     4741 ccaattatat accagctaga atatctgtta ttcttatgac aattggaagt ttctttttaa
     4801 attataacta taggaactgt tttaaaatct ctattagaga tagaaagaat cataaaagtc
     4861 caaattgtgc tttttcagaa ggtgcagtat ctggtgctct aggaatacaa ctaggaggta
     4921 ccaatgttta ttttggggaa aaagtatata agccaacaat aggggataag cttagggaga
     4981 tagataaaga agatataatt aaaactaata aaattatgta tgcatcttca tttgtatcta
     5041 tgttggtatt tacactaata tatgtgttat gtaggatgat tttaattaac ttaattatat
     5101 aaattattgt ttgttaactt aagggggaca tagatgaagg atttagggca tggtgccaat
     5161 gtagatgaaa tggcaagatt atatgggaaa aacccaaaag aaataattga ttttagttca
     5221 aacataaatc caaatgtatt accaaattta gagagataca ttttaaaagg attagaagag
     5281 tgtaggaatt atccagatat aaattataca aacttgagag aaaatatatc aaaatatata
     5341 gatataaatc cagactttat aatacctggg aatggtgcaa cagaagtgat ttatttgctt
     5401 atgaagagta ttaaaaaaaa attggctata ataaatccga ctttttcaga gtatagaaga
     5461 agtgctgagt taaataattt agatattata gatttagagt tagatttaga aaataacttt
     5521 aaacttaata tagatataat aaaagaaaat ataaaaagat ttgatagttt atttatatgt
     5581 aatcctaata atcctagtgg taatgtccaa gatttaaagg aacttgttca tttactagat
     5641 aaacataata aggtactaat aattgatgaa actttcatgg aatttgttga agatgaaagt
     5701 aaatatagtt tggttaagta tatagaatca aataaaaata tatttataat taaagctgtg
     5761 actaagtttt ttggtatgcc tggtttaagg ttaggatatg gtcttacaag caacactgaa
     5821 attatgaaca aaatttatga acacaaagag ccatggacta ttaattcttt tgcagatata
     5881 ctatctaatt ttatttttga ggataaagag tatattaaaa gtagtaaaga atattacata
     5941 gaagaaagaa aatatatgtt acaagagttg agaaacataa gaaatataaa ggtctatgat
     6001 acagatgcta attttatttt gataagaaca tataaaaaaa caactaaaga attgaaaaaa
     6061 gatttgttta agcaaggaaa catattagtt agagatgcgt ctaattttat aggattagat
     6121 gatagtttta taagagtggc tataaaatct catgaagata acaaaattct tatagaaaat
     6181 atgaaaaatt tattgggtga ttaaaatgaa atcttatgga atatgcccag catcttgtgg
     6241 agaatttgtg caaggtataa ttgatgatga agaatattta tgttcatatg caatagatat
     6301 gtattctaaa gtgtatattg aagaaaaatt agtagatata aatttaggga gatataagtc
     6361 aaggttagca attgaaaagg tatttgaaaa atttaattta cctaaaaaat atactaaaaa
     6421 tatttcttta aatataaata gtaaaatacc tgttggaaaa ggaatggcaa gttcaactgc
     6481 tgatatagga gctaccataa aggcaacatt atcattgata gataaagatt taagtagtga
     6541 ggagatttct aaattagcag cagaaataga accaacagat tctatattta tagataaaaa
     6601 tagtatattt aatcctctaa atggaactgt tataaaatac ctaggaaatc tgaccaatgc
     6661 gaaagttgta atacttgaac caaacaaagt actagatact atgaaaatta gattgaggca
     6721 agattataat agattaaaag tagagaataa agaagtcata aaaaaatcat ttgctctttt
     6781 agaagaagga ttgaaaaaaa ataatttatc tctagttgga gaggcttgca ctttgagtag
     6841 cttagcaaat gagaatatag aaaaaaaaga gtatttaaat gaaataataa aaatatcaaa
     6901 aaaatatgga gcatatggag taaatattgc tcacagtgga actgtaatag gtattttaac
     6961 tgataagttc atgaatgata aaaagatgat agatgcatta tgtgaatcta acataaattc
     7021 tgtctacaat aaaatttata cactaaacat aattaatgga ggaattaagg gggaaataga
     7081 atggaataca taaagaatcc tatgaaaata gaagaaaaaa gttttgaatt gatacaagaa
     7141 ataatagacg aaattagacc agattataaa tttaagaata atatcgaaga aaaaataata
     7201 aagagagcta tacatactac tgcggatttt gattacttag atatattaaa aatatcagaa
     7261 gaagcagttg atagtataat agatgcatta aaaaataatg caagtattta tactgataca
     7321 aatatggctc ttagtggtat aaataaaaga aagttagaat cattaggatg taagtataaa
     7381 tgtttagtgg cagatgatga aactgcaaaa ttagctaaag aaaaaggaat tactcgttct
     7441 atggcagcag tagaaattgc agctaaagaa gaaggaagaa aaatattcgt tttaggtaat
     7501 gcaccaacag ctttatataa agttatggag atgaaatcag aaggtagatt agatttagat
     7561 gcagttatag gagttcctgt aggttttgta ggagcacaag aatcaaaaga tgaagttgaa
     7621 aaaacagata taccatatat aatttctaaa ggtagaaaag gtggcagtaa tttagcagca
     7681 gcaatagtaa atgctatatt atatactatg taggtaccta tatggaagaa tatgtatata
     7741 tagatggtaa aaaatataga agaggatata ctacaggttc atgtgcaaca ggtgcatcta
     7801 aggctgctgt atatatgttg attacaaaaa atagaatcaa tactataaat atagatactc
     7861 ctaaggggat accattacta ctaaaagttg ataatataaa tatttctgat acatttgtgg
     7921 aatgttccat aaaaaaagat ggtggagatg atatagatgc aactcatacc atggatattt
     7981 atgcaagagc tgaaattgta tctaaaaatg ataagaacaa agggtatctt actctaaagg
     8041 atatagatag tttaagtacc aatagtgagt gtaaaagtga gctctataag tttattaggg
     8101 tttatggcgg aactggtatt ggtgtggtta cgaaaaaagg gcttagtgtg gatgttggta
     8161 aacctgctat aaatccgact ccacttaaaa tgataaatca tgaaataaga aagcttatag
     8221 gagataattt tgaatctata cttggaaatg ataaagtttt aaaaataaca atctttgctc
     8281 cacaaggaga aactgtagca aaaaaaacat ttaacccaag attaggtata gttggaggaa
     8341 tatctataat aggtacaact gggattgttg aacccatgag tgatgagggt tggaagaagt
     8401 ctctatctat agaacttcaa atgaaaaaag aacaaggctt agataaaata atattggttc
     8461 caggaaatca tggagaacag tttataagag aaaaattaaa cttagatatt aagtatgtag
     8521 ttagggtaag taattttata ggctatatga ttaaagaagc tcaaagaata ggatataaaa
     8581 aaatcttaat ggcgggtcat ataggtaaat ttataaaagt atctgctggt atttttaata
     8641 ctcatagtaa ggtagcagat gctagaagtg agatattagt ggcaaactta gctttaatgg
     8701 gagctagata cgaattttta aataaaataa atcaatgcgt aactactgaa gaagctgtag
     8761 agctaataaa tagtagtgaa tatagagaag tatataatat attgagtaat aaatgtaggg
     8821 agagagtaaa gcaatacttg aatgaagatt cagatgatat tgatgtggaa gtaatcatat
     8881 tttcaatgga taagtcttta ttaggtaaat ctgataatac agatgattta gtggaggtct
     8941 ttatatgata aacataatag gtctaggacc tggcaactta gactatataa cgaaaaaagg
     9001 tgaaaattta atttccactt ctgatgtatt aattggtggt aagagaaatt tagagtctat
     9061 aaaaaacttt gaaggagaaa aaatagtctt agattctaat ttaagagaaa ttatagagta
     9121 cataaataac aataaagaaa agcaaatatc aataatagca tctggtgacc cattaatata
     9181 tgggataggt agatatctat ctaaaaatat tgataataag atgctaaata tggtttctgg
     9241 gattagttca ttacagtaca tattttcaaa aatatatgta gatatgaatg atgtatatat
     9301 tacaagtagc catggtaaag taccagattt tgattacata ttatcacata aaaaagtatg
     9361 tatggttaca gactttaaga taggaccaaa acaaataagt aaagaaataa tagatagaaa
     9421 tctaaataaa attattgttg taggagaaaa tttgtcatat gataatgaaa aaataacaat
     9481 agcaaaacca gaagaaataa taagaatgga caattttgat atgaatgttg tggtgatttt
     9541 agatgaggaa tagtgaattt ataacaggta aagttccaat tactaaggaa gaggttagag
     9601 caatatcaat aagtaagtta aatttagtaa atgctaaaaa cctcatagac ataggggctg
     9661 gtacaggaag tgtaagtgtt gaagctgcat ataattatcc taatttaaaa gttatatcta
     9721 tagagaaaaa tgatgatgct atagagctta tagagaaaaa tataaaaaaa ttcaacttaa
     9781 aaaatataga agtgataaaa ggatatgctc caattaatct agggttaaat gttaaagttg
     9841 agtctatttt tcttggggga acaggaaata atctggaaga aataatagaa tggtctaaaa
     9901 aaaatctcgt tacaggagga agacttgtag ctaattttat aataatagat acatttaatc
     9961 aaactttaaa actgcttaaa aagcatggat ttaaagaaat agatgtatgt gttttgaatg
    10021 tttctaaact agagaaacta ggaaaaggtg aatattttaa accactaaat cctatatata
    10081 taatatcttg tgaaaaaggg gaaaatgatg atgaataaag tacattttgt aggagcagga
    10141 cctggagata aagagctgat aacattaaaa ggatacaaat tattaagtaa tgcagatgta
    10201 gtgatatatg caggttcact tgttaatcct gaacttttgg aatactgtaa agaagattgc
    10261 caaatacata atagtgcaca tatggattta caagagataa tagatgttat gagagaaggt
    10321 atagaaaaca ataaatctgt tgtaagactg caaacaggtg atttttctat atatggttca
    10381 attagagaac aagtggaaga tttaaataaa ttgaacatag attatgattg tactcctgga
    10441 gtaagttctt ttttaggggc agcatcatct ttgggggttg aatatacagt tccagaaatt
    10501 tctcaaagtg taattataac aagaatggaa ggcagaactc cggttccaga gaaagaatca
    10561 atacaatcat atgctaagca tcagacatct atggtaatat ttttatctgt tcaagaaatt
    10621 gagaaagttg tgtctaaatt actggaaggt ggatatccaa aagatactcc tatagcagtt
    10681 atatacaaag caacttgggc agatgaaaaa atagttaaag gaactttaag tgatatagct
    10741 gtaaaagtta aagaaaacaa cataaataag actgctttaa taatggttgg gagattttta
    10801 ggagaagagt ataataattc aaagttatat gataaggatt tcaaacatga atatagaggg
    10861 taatattgct ataatctgta ttacagaaaa cggaaaaaaa ttagctgtaa agataaattc
    10921 tctagtaaat aactgtatta tatatcaagt aaaaaataaa gaaagtaatt tagctgttga
    10981 agaaacatgt gtacatacag ttcaaaagaa gttaagtgat tttgtaggag aaatatttag
    11041 taaatttgat tacatagtat ttataatggc tacaggtata gttgtacgaa gtatagcgcc
    11101 atacataata agtaagttta gtgaccctgc aattttggta atggatgaaa aaggcaaaaa
    11161 cgtaataagt ttattgagtg ggcatattgg aggggcaaac agcttgacac agtacataag
    11221 tagtcttatt gatgctaatc cagtaataac aacagctact gatataaatc aaaaagcttc
    11281 cttagatatg atagcaaaaa aactaaatgc acatatggat aattttagag aaagtgtaaa
    11341 agaagtaaat tctatgctag tgaataataa atgtgttgga ttatatgttg atgaaggata
    11401 tcaagttgat actaggggat ttaaacttat agaaagtaag ggtgagttaa gagatagttt
    11461 ttcaaacacg tatatagaaa aattagttgt agtaagcaat aaaataaatt gtattgaaga
    11521 tattgagaat ttaaacctag ataataaact tgaacattgt tatagtacta aaaaaataat
    11581 aaaggtaatt ccaagagata tagtgatagg aattggctgt agaagaaaca cagatagtga
    11641 gctgatgtat aattcacttg aagagtttct taataaacaa aatatagata ttagagcaat
    11701 aaacacagtt ggaagtatag aactgaaaaa agacgaaaaa gcaataattg aattatcaaa
    11761 aatattaaaa gtgccattta aaacattttc aatagatgaa atatctaagg ttgataatct
    11821 ttttgataaa tctgattttg ttaaaaaaaa tgtgggcgtg tattcagtag cagaacctgt
    11881 agcatatctt ttgagcaaag gtaatttgtt agttgaaaaa cataagtata aaggaataac
    11941 tttttcagtg gggagaataa aaatatgata tacgttgtag gaattggacc aggaagtaaa
    12001 gatacaatga ctttagaagc aataaaagca atagaagatt ctgaggctat tgttggatat
    12061 aagacttata taaaactaat agaagaattt atacaaggta aagaaataat ccaaaatggt
    12121 atgaggcaag aagttgatag atgtaaacaa gcggtagagg aagcaaaaaa aggaaaaaaa
    12181 gtagcagttg taagcagtgg tgatgctgga atatatggga tggcaggtct tattttagaa
    12241 ttaatatcta aagaagatga agatataaaa gtaaaggtaa ttccaggtgt tactgcaagt
    12301 attggtgcag cagctatact cggagcgcct attatgcatg atttctgtca tataagtttg
    12361 agtgatttaa tgactccatg ggaagtaatt gaaaaaagat tgagattggc tgctgaagct
    12421 gattttgtaa tttgtttgta taatccaaga agtaagggaa gaagtgaaca tctaagcaaa
    12481 gcatttaaaa taatgggtga gtttaaaggt ggagatacac ttgttggagt ggtaaaagat
    12541 gtaggtagag aaaaagaaga aaaatttgta tgtacttttg aaaatatgga ctttgaaaaa
    12601 gtcgatatga ctacaatggt tataatagga aataagtcta cctatattaa tggtgaattg
    12661 atgataactc caagaggtta tacagtatga ttttaatttt aggaggaact tcagatagtt
    12721 tatctatatg tgataagata aatgaactga aaaatcaacc atatatcctt tcagtgacta
    12781 cagattatgg aaggaaactt gcacaacaac atgcagagaa tgtaatttta ggtaagcttg
    12841 gaaaagaaga tatgctaaaa tttataaaag ataataatat agtaaaaata atagatgcca
    12901 ctcatcctta tgcagttgaa gtatccaaaa ctgctattac ttgtgcaaaa ttattgaata
    12961 tagattatat aaggtttgag agaaaatctc taattgaaga aatagattat gataataaat
    13021 ttatagtaga tacaattgaa gaagcctgtg aaatagcaaa taaaataggg acaaatatat
    13081 tcataggaac aggaagtaaa aatttgaaca gatttataga ggaaataagt gataaaaatt
    13141 tagttgttag agttcttcca acgagtgaag tcatattaag ttgtgaaaaa ttaggtttaa
    13201 gtgcagataa tataatagct atgaaaggtc catttagcca gcatataaat gaagaaacat
    13261 ataaacatta caatatagat ttagttataa caaaagaaag tggaatagct ggtggattct
    13321 tagagaaggt aaatgcatgt aaaactttca atataccagt aataattata aaaagagaat
    13381 cgattaacta cgaaactgta tttaatgaca taaataaaat taaagacata cttgtgtaat
    13441 ttttaggagg agaatatatg aagaaagcct tattagtagt gagttttggt acaagctatc
    13501 atgaaacgag agaaaagaca atagatacat gtgaagataa aataaaaaat tcattaaaaa
    13561 gctatgattt ttttagagcc tatacatcaa atatgataat aaataaaata aaaaaaaggg
    13621 atggaataga aatagataat ccaatacagg ctttagataa aatatatgag caaggatatg
    13681 atgaagtaat aatccaaaca ctccatatta tatgtggcga agaatttaac aaattaaaag
    13741 aacaagtaga tggatattct tctaaattca aaaagattgt attaggaaga cctttgctta
    13801 cacatattga agattatcag gaagctgtag aggcaattaa gcatcaaatt cctcatatgg
    13861 aaagtaatga agcagtagta tttatgggac atggaacact tcatgaatca cattcagcat
    13921 atccagcatt ggaatacatg cttagggata atggaataaa tgcttatgtt ggtacagttg
    13981 aagggtatcc agaaatagaa catgtaataa gaagattaaa agaaggaaat ataaaaactg
    14041 taaatttgat gccatttatg ttagttgcag gagaccatgc tataaatgat atggcaggag
    14101 atgaagaaga ttcttggaaa acaatattag aagaaaatgg atttgatgta agagttcatt
    14161 taaaaggatt aggagaaaat tcttatatac aagataaatt tgttcgtcat gctgtaaaat
    14221 gtgcagagaa tgctaagttc tatggtatag gagcaggacc aggagatggt tctttgctta
    14281 cgataaaagc ggttaatact ttaaagaaac tggatattct atacactcca gaatcaaaaa
    14341 agggtggaga tagtctagct ttatcaatag ttagtgaata cttacctgaa tcagtggaaa
    14401 taaagtctag gcatttccca atgagttttg atgggaatga aaaaagttta gcttgggaca
    14461 atgtggcaca agaaattata tcagatgtta atgatggtaa aaatgtaggg tttgttacat
    14521 taggagaccc tatgatttat agtacatatg tatatataat gaaaagaata ttaggagagg
    14581 tagaagtcga aactatacca ggaatttcat cattttccaa tatagcatct aatcagaatt
    14641 ttccattagt catggataaa gaagccttag tggtaattcc ttgtactatg gaagatgata
    14701 agatagagta tgctttgcaa aattataatt caatagtatt gatgaaggtg tataaaaatt
    14761 tcaaagaaat aataaaaaaa ttagaaaagt atgatttaat agaacatgct attttagtaa
    14821 gtaattcttc ccaagaaagc gaaaatgtgt ttacagattt aaaagaagcg caccttgaag
    14881 ataagatatc ttatttttct actatactag taaatagaga taataaaata aaataggtga
    14941 aagcaatatt taaaggagac ttaatatgaa tatagtggtt ggaactaggg gtagtaatct
    15001 agcgcttata caaacagaat gggtaataaa tgaattaaaa aagaagtatc cagaaattag
    15061 ttttgagatt aaaatcataa agacaaaagg agatttaatt caaaatgttt cactggataa
    15121 aataggtgat aaaggattgt ttgtcaaaga aatagagcaa caattattag atggaaaaat
    15181 agacatagca gtacatagta tgaaagatat gccttcttat ttagcagatg gactaaaatt
    15241 tgctcatacg cctaagagag aggacccaag agatgttcta atacttagag aaggatataa
    15301 aaatttagat gatttgcctc atggagcagt aataggaaca ggaagtaaaa gaaggaagtt
    15361 tcaattattg aaacaaagac ctgacttaaa tatagtacaa gttagaggta atgtggaaac
    15421 taggatacga aaaataaaag atgaaaatat gcatggtatt gtgttggcag catcaggaat
    15481 aattagagct aatttacaag ataaaataag ttcttattta cctgttgatg ttgttattcc
    15541 agcacctgcc caaggagctt tagctataga aattagaagt aacgatagta cgatagaggg
    15601 aatagtaaat tcactgaagg atgagaatac agaaatccaa atattagctg aaagaggttt
    15661 tttagatggt gtaaatggaa gttgtcatat tccaatggca gcttattgtg aaataatcca
    15721 agataaaata catcttactg gcttatatgg agattcagaa ggaaaaaaag tagtaatcaa
    15781 gtctatagat ggagatattt cttctcctag agagttaggt ttaaagttag ctaaacttgt
    15841 attgaaggag tatgagaatt atgaagggta aggtttactt agtaggggca ggaccaggtg
    15901 attataaact catgacttta aaaggtttag agtgtataag aaaatcggat gtaatagtat
    15961 atgatagatt agcaaatagt agttatttaa gagaagctaa acctgattgt gaacttatat
    16021 atgtaggcaa ggcatcaagt aatcatattt tgccacaaga agatataaat agaattatag
    16081 cagaaaaagc taaagaagga aaaattgtaa ctcgtcttaa aggaggagac ccttatgtat
    16141 ttggtagagg gggagaagaa gcagagactt tagtagatga aggtatagaa tttgaagtag
    16201 ttccaggtat aacttctgct ataggaggtt tatgttatgc gggtattcct ataacacata
    16261 gagaccatgc ttcgtcattt catgtaataa caggtcattt gaagggtgat gatagtggag
    16321 aattaaattg gaatgctctc gcaaataaca aaggtactct tgtgtttttg atgggaattt
    16381 caaacctaga gaaaataagt gaaaacttaa tgaaagaagg aaaagataag gatacacctg
    16441 ttgctcttat aagttgggca actagatata atcaaagagt tgttacatct acacttgaaa
    16501 atgtttataa aactgctatt aaagaagaag taaaaccacc aactcttata gtagttggaa
    16561 gtgttgtagg acttagagaa aagttgaact tctttgaaag aaagtcttta tttggaaaaa
    16621 gtattgctgt aactaggtct aggaatcaaa atagcgtatt agtagaaaaa ataatggatt
    16681 taggtggaaa tcctatagaa atacctacta ttaagataga aaagatacaa aataatatta
    16741 aattagaaaa tgaaataaaa aatataaata agtataatta tctaatacta actagtaaaa
    16801 atgccgtaga aatcttcttt gaaaagatat atgaaatgaa ttttgattta aggatactat
    16861 ctaatttaaa ggtatgtgct ataggttctg ctacatcaaa tgagttgaaa aaaagaggaa
    16921 ttatagcaga tatagtacca aaaaaatttg tagcagagtc actgtatgaa gagttagcac
    16981 ctattttaaa tgataacgat aatatactta taccgagagc agaaaatgct agagattatc
    17041 tggttgacaa acttaaggct atttgtgaag taacagaagt acatatatat agaacagtaa
    17101 tagatgagga taaaaaaggt gagatattag atattttaaa ttctaatgat atagattata
    17161 taacatttac tagttcgtca acagtaaata atcttgttga aattatagga aaagaaaata
    17221 ttaaaatatt agaaaaaata aaaacactat ctatagggcc tataactaca gagacaataa
    17281 ataggttggg aataaaattg tacaaagaat ctgctacatc tactatagat tcgttagtag
    17341 atttaattat aaaaaattaa ttttggaggc ttgaatatgt taagaagacc aagaagacta
    17401 agagcaagta aagcaatcag aaatttggta agagaaacta aggtgaatgt agaagattta
    17461 atatatccac tgtttgtagt ggagggggaa aatataaaag aagaaattaa atctttacca
    17521 gatgtctatc atttatcaat agacatgtta gaagaagaaa taaaagaaat aagtgagttg
    17581 ggaattgaac atataatgtt atttggtgtt ccagatgaaa aagatgagtg tgcttcacaa
    17641 gcttttaatg ataatggaat agttcaaaag gcaattagaa aggtaaaaga aataaattca
    17701 gatataaatg taataacaga tgtctgtatg tgccaatata caagtcatgg acattgtggg
    17761 attttaactg atactggtta tgttgataat gataaatcat tggagtattt ggctaaaata
    17821 tctgtaagcc atgctaaagc aggagctgat atggttgctc catctgatat gatggatgga
    17881 agaatacaag caataagaga agctttagat gaaaatggat tagaaaatgt tggaatcatg
    17941 gcttatagtg ttaagtatgc ttcaaattat tatggacctt ttagagaagc agctcactca
    18001 gcaccatctt ttggagatag aaaaacatat caaatggacc cagcaaatag caatgaagct
    18061 ataatagaga ctgaacttga tatattggaa ggtgcagata tacttatggt taaacctgca
    18121 ttatcatatt tagatattat aagaagagtt aaagataatt ttgacttacc tctagcagct
    18181 tataatgtaa gtggagaata ctctatgctt aagtttgctg tgaaggaagg tttattaaat
    18241 gaagatgtaa ttcttgaatc aattacatct ataaaaagag caggagcaga tataataata
    18301 acttattttg ctaaagacat tgctaaactg ttgaaaaaat aaattctagt agtataaaga
    18361 atatcaaaaa gtataggaat tgtaattaac tattatacag tagttttgtt tagacattta
    18421 ttgaagaata atagaaacta atttgcaatt tctatacttt atttatgtaa ctaaataaaa
    18481 tttggaggta gaaaatgcta tatcctatta atttaaaatt agatgagtta gatgtagtta
    18541 taattggtgg tggagaaatt gcatatagga aatgtaaaaa ttttttagag tttaacaaaa
    18601 atgttaccat agtttctaaa caaattttaa ataaatttta tgatttaaaa ggaaatataa
    18661 aaataataaa agatgattat aaagaaatat atataaaaga tgcttctgta ataattgctg
    18721 ccacaaataa tagagagtta aatatggaaa taggattata ttgtagagca aaaaataagc
    18781 tagtgaatgt agttgataat atagaaatat cgaactttac tgttccatca tatataaaaa
    18841 gaggagattt acttataagt gtatctactg ggggcaagag tccttcatta tcatcaaaga
    18901 taaaaaaaga gattgaagag atatatactg aagattatga ggagtattta aatgtattag
    18961 gagatataag aaaagaggtt ataaaaaaat acgaagataa aagcaaaaga aaagatttgc
    19021 ttaatatgtt gataacactt gacttagaag aacttaaaaa gtttgatata taaaaaataa
    19081 aataaaaaat gttaaaaagg tgttgacgga ttatttttaa gatgatatag tattacttgt
    19141 ccttaagaaa agggcaaaca caaaaa
//
LOCUS       DAEMAE010000056        19143 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEMAE010000056 DAEMAE010000000
VERSION     DAEMAE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.057"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..366
                     /locus_tag="KPH93_001577"
     CDS             28..366
                     /locus_tag="KPH93_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG1688405.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(494..1786)
                     /locus_tag="KPH93_001578"
     CDS             complement(494..1786)
                     /locus_tag="KPH93_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1688406.1"
                     /translation="MKRNLSLLLICLLIFTSFLGRSNISFADNEPAIVAKHAVLMDYE
                     TGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKEGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYKIFLEKNESKGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILILLVLFIITSNIKKKKKIKKARGKRNMKK"
     gene            1866..2570
                     /locus_tag="KPH93_001579"
     CDS             1866..2570
                     /locus_tag="KPH93_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG1688407.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSRIFKDDIYVVSKKNLPNE
                     KTHVVETEIKGEDNNHNKATKDIDKNFQNNKITIYISGAVNRPGIVTIESYKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICID"
     gene            2662..3708
                     /gene="selD"
                     /locus_tag="KPH93_001580"
     CDS             2662..3708
                     /gene="selD"
                     /locus_tag="KPH93_001580"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2710..2712,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG1688408.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVNPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFDK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKNVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNVEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3726..5138
                     /locus_tag="KPH93_001581"
     CDS             3726..5138
                     /locus_tag="KPH93_001581"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG1688409.1"
                     /translation="MIILSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCSASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     DAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNASINI
                     EDGFSQVGGGSMPLETISTKVIAITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5252..7135
                     /gene="selB"
                     /locus_tag="KPH93_001582"
     CDS             5252..7135
                     /gene="selB"
                     /locus_tag="KPH93_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG1688410.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRSYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDRVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNEKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7240..9978
                     /locus_tag="KPH93_001583"
     CDS             7240..9978
                     /locus_tag="KPH93_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG1688411.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLIATGIIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYV
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYNEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVESILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNVEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSRDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLERIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYNDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRSEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10097..11041)
                     /gene="manA"
                     /locus_tag="KPH93_001584"
     CDS             complement(10097..11041)
                     /gene="manA"
                     /locus_tag="KPH93_001584"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG1688412.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWDKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHIQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11214..12575
                     /locus_tag="KPH93_001585"
     CDS             11214..12575
                     /locus_tag="KPH93_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1688413.1"
                     /translation="MELFKTESKKYEKEFLKLLEEWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNIEKLGKDNICFDINNISINYDNGNYIVHGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIMKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRLIDAIGIYAEAIITLC
                     NKI"
     gene            12663..14552
                     /locus_tag="KPH93_001586"
     CDS             12663..14552
                     /locus_tag="KPH93_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1688414.1"
                     /translation="MITKKHAVIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIYGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLRKKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIESLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDEEFMVAISDHIKRFLNR
                     AKANIKSNNPLLHQIKEKFPIAFNLAVFLSNKLEAEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVILSLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14567..15022
                     /locus_tag="KPH93_001587"
     CDS             14567..15022
                     /locus_tag="KPH93_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688415.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNIEEISNLINEALGL"
     gene            15081..15398
                     /locus_tag="KPH93_001588"
     CDS             15081..15398
                     /locus_tag="KPH93_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG1688416.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15423..16685
                     /locus_tag="KPH93_001589"
     CDS             15423..16685
                     /locus_tag="KPH93_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG1688417.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRIVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16790..17854
                     /locus_tag="KPH93_001590"
     CDS             16790..17854
                     /locus_tag="KPH93_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1688418.1"
                     /translation="MNKIEKVREYFIEYDIDGFLINSPTNKFFVGNLFSSSGYVFITK
                     EAQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLREIKNEDEIKYIKKACEIVDATFYHIIDFIKVGMTE
                     KQVENEIVRVIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMSTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YADNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17908..19086
                     /locus_tag="KPH93_001591"
     CDS             17908..19086
                     /locus_tag="KPH93_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG1688419.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVHVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIEKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMNISGL
                     GISASEFTDRLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7709 a         1996 c         3343 g         6095 t
ORIGIN      
        1 aatatcaaat aaatgggagg aaatctaatg aacttattag tgattggtgg tcatgaaaga
       61 atggaaaaag attatatggt tatggccaag aaaaaaggtt ataagacaaa ggtatatact
      121 accatgtcat ctaaattaaa taattctata ggaagaccag atgcagttgt catacttaca
      181 tcaactgttt cacataagat gtctagaacg gttgagtctc aagctaaaaa acaagatata
      241 ttaatcgtaa ggcataaaaa tagtagtaaa gtagcattta atgaatgcct agatatggtt
      301 gatgaatgtc tgggaaattg ttcaaaatgt aagcttaaag acaaacaaaa acaaaaaaat
      361 atataggtat aaaaaataca taaacataaa attaaaagta ctgagataat agttataatt
      421 attatctcag tacttttaat tttataagga gaagcatatt aataaatttt ttattataat
      481 ttttaaataa ttgctatttt ttcatatttc ttttgcctct agcctttttt atcttttttt
      541 tcttctttat atttgaagtt attataaata aaactaaaag tattagaata cctgcaataa
      601 tcttaacaaa agtcattaaa acattatttt cagtcaaaaa tggaagactg ctatcaagat
      661 tattttttgc aattaagtct atactgtttt ctagtttacc attattatat acatttaaag
      721 taccaacctt agcccctttt tttataggca aatcaatttt atttaactct gtttttgtat
      781 cataattgcc tttagattca tttttttcta agaatatctt ataactattt ttgggttcgt
      841 aaatcaattc atgttgtttt gtaaacaata cttttttagt atctgtgtat ttttctttat
      901 caacaatagt agcacactta aagttatcaa atccataatc aattagaata cgtgagtcta
      961 aatataaatc agcattagtt gaattaaata cagcaacgat aactcttctg ccatctttta
     1021 cagcactcga taaaagacat ttaccagcag catcagtata tccagttttt ataccatcaa
     1081 ctatatcata ttttatattt ataggctgac ctttgtatgt aatcttatcg tgtgatgtca
     1141 aaaacttatt agtgtttaca aagtatctct cataaggata tgcttttgta gcttcaaatt
     1201 taagtgactt agtttttaca atttgtctaa aagtatcatt attcatagct tctctagcta
     1261 tgagagccat atcatgagct gttgtgtagt gatttgggtc tggtaatccg tgaggattat
     1321 taaaatgagt attagtagcc ccaatttttt ttgcctcaga gttcataagg tcaataaatt
     1381 tttgtacatt tccaccacca acatatctag ctaatacaaa agcagcatca ttagcagaat
     1441 gaaccaaaag agcatctagt aactgcttta cagtaaaaga ttccccctct tttagataca
     1501 tactagaacc atctatttga ggtaaatctt taatttctat tacttgattt aaatctttaa
     1561 cctcctttag aactaaacaa gctgtccata ctttagttgt tgaagctgga taaagcttag
     1621 aatttccatc cttattatat agtattttgc cagtttcata atccattaaa actgcatgtt
     1681 tagcaactat agctggttca ttatctgcaa aagagatatt agatcttcct aaaaatgaag
     1741 taaatatcaa taaacagatt aataaaagtg ataaatttct tttcattgtg agcctccaaa
     1801 ttttaaattc atcttttagt atagcatata tttgtcaata attaaaagaa aggagtgatt
     1861 aatatatgaa aaaaaaggta aaatttattt cgttttttgt tttatttatt ttaataatat
     1921 catcaataat cgtttataaa tcgcgtattt tcaaagatga tatttatgtt gtaagtaaaa
     1981 aaaatttacc taatgaaaaa acacatgttg tagagactga aattaaaggt gaagataata
     2041 atcacaataa agcaactaaa gacatagata aaaattttca aaataataaa ataactatat
     2101 acataagtgg agcagtaaat agacctggaa ttgtaactat agagtcatac aagagacttt
     2161 atgatgctgt agaattacta ggaggaacga caaaagaagc cgatttaaat ggagtcaatc
     2221 tatcagtaag attagaagat gaacaacatt acataatacc taaaataggt gaagcaacat
     2281 ctgttacaag taatgatgac tccaataaac caaatcagaa gaatgagtca agaggtgagc
     2341 ctaaaaataa atccaataaa aatatatcta acgaatcaaa agtaaatata aatgtagcga
     2401 ctatagaaga gttggattct ctacctgggg ttggagaagc tacagccaat aaaattttac
     2461 aacacagaga agaaaatgga cagtttagtt caattgagga aataaaaaat gtaaatggta
     2521 ttggagataa aaagtatgaa aatataaaag atttaatttg tatagactaa aatataatac
     2581 aaaacttact tatagatatg ttattataaa tatgtatgta tatgtgtgta tatgtggaaa
     2641 tttagtaaag gaggcacttt tatgaaaaaa gaacaaaaaa aacttacaga gatgacaaca
     2701 gcaggtggtt gagccgctaa aatagggcca gaggttctgg catcagtttt atctcaattg
     2761 cctaaaaatg ataatataga aaatttatta gtaggactag atacagcaga tgatgctgca
     2821 gtttataaat taaatgatga tatggcactt attcaaactt tagacttctt tacaccaatg
     2881 gtagatgacc catatgtatt tggtcaaatt gctgcatcaa attcactatc agatgtatat
     2941 gctatgggag gcaaaccttt ggttgctatg aatattgttt gttttccatc atgccatgat
     3001 atggatgtac ttgcagaaat attaaaaggc gggtttgata aagtgaaaga aagtggagca
     3061 cttttggttg gagggcatac tgtagatgac aaagagccta aatatggtct ttcagtttct
     3121 gggattgtaa atccaaataa agttttatca aatgctactg caaaacctgg tgataagtta
     3181 attataacaa aaccaattgg agtgggtgta ttaaacacag ccatgaagga aggtatggta
     3241 gaacaacata tagcagataa agtaatagaa ataatgatac atttaaataa atatgcagca
     3301 atgagctttg ataaatttga tgtaaattcg gttacagata taactggctt tggtctactt
     3361 ggtcatactt tagaaatggc caaagcttcc gaagtaagta ttgaaattga atcaaaaaat
     3421 gtaccaatta ttgaaggtgc aatagaaatg gcacaaatgg gaattatacc tgctggtatg
     3481 tataaaaata tgcattatgt atctaaagat gtagaagttg taggaaatgt agaagttgca
     3541 gttcaagata ttttatacga cccacaaaca tcaggagggt tgttaatttc ggtaaaagaa
     3601 gaacttgcag aggaacttgt aaaagatatg aaattaaatg gagcaataga agctaaaatt
     3661 ataggttctg tagttccaaa aggggaaaaa tatattacag ttttataaaa tattatagag
     3721 aaagagtgat tattttgagc aaaagagaat tgtttgcaat gttgccttca gttgatgaag
     3781 tattaagtga taatagaatt gtagaaataa taaatgaata tcctagaagt ttggttttag
     3841 aatctgtgag agaagtaata gatttaaaaa gacaatttat attaagatta aaagaagatg
     3901 catcaaattc tgtaacaata gagtttggtg aaataataga atctgcaata gagagagtta
     3961 agttaaatta ctcattatct ttaaaaaaag ttataaatgc aactggtacg gttattcata
     4021 caaatttagg aaggtcttta cttagtgaag atataaaaga tgaattatgg tgttctgctt
     4081 caagatattc aaacttagaa tacgacttgg ataatggaga aagaggttca agatactctc
     4141 atttaactag tacaataaaa agacttactg gagcagaaga tgtactagtt gtaaataata
     4201 atgcagcggc agtacttctt gtacttagca caatggctaa aggaggagaa gcaatcgtat
     4261 ctcgtggaga attagttgaa gtaggtggct catttaggat tccaagtata atggctctta
     4321 gtggtgcaga attagttgaa gtaggttcaa ctaataaaac tcatctgaaa gattacaaag
     4381 atgccattac agaagataca aatgtattga tgaaggttca tacaagcaat tatagaatca
     4441 tgggattcac agagagtgtt tctatagaag aattagttaa tttaggtaaa aaatataaac
     4501 tgcctgtaat agaggactta ggaagtggag tttttataga tttatcaaaa tatggtttat
     4561 cttatgaacc aactgtattg gattctattc gacaaggtgc agatgtagta acttttagtg
     4621 gagataagat gcttggaggt ccacaagcag gtattatagt tgggaaaaaa gagtatatag
     4681 aaaaaatgaa gaaaaatcaa cttactagag cacttagagt tgataagctt acaatatgtg
     4741 ctcttgaagc aacattgaga atgtatttag atgagacaaa agctatagaa aatataccta
     4801 ctttaaaaat gttgacatat aaaattgaag aattagaagt aaaagcaaat aaactatttg
     4861 aaaaaataac tgctttaaac ttaaatgcaa gcataaatat agaagatgga ttttcacagg
     4921 tgggaggagg gtctatgcct ctcgaaacta taagtacaaa ggtaatagca ataactcctg
     4981 aacatatgaa tgtttcatct ttagaaaaaa aactaagact tagtgaagca catataatag
     5041 ccagagtgta tgataataaa tatgtactag atgtaagaac tatatttgat gatgaatttg
     5101 atgtaatagt tgaggaattg agaaaagcat ttaattaaat aaatttgggt taacaaatat
     5161 taaatttaga agtataagta tgaagttttg attaaaaaat agctacacac acttatgact
     5221 ttagtcatag gaggtttaga gggggcaaaa aatgaaaaat gtagtaattg gaacagcagg
     5281 tcatattgac cacggaaaaa ctactcttat aaaagcattg actggaagag agactgatac
     5341 attagatgaa gaaaaaaaga gaggtatttc aataaactta ggatttactt actttgattt
     5401 accaaacaat agaagagctg gaattgttga tgtcccagga cacgaaaaat ttataaaaaa
     5461 catgttagct ggagcatcag gaattgatat agttttactt gttgtagcag cagatgaagg
     5521 ggttatgcca cagacagttg agcatttaga tattctaagt ttcttaaata tcaaaaatgg
     5581 aatagtggtt ctaactaagt ctgacactgt agatgatgaa tttagagagc tagtaaaaga
     5641 agatatttgt gaaaaaataa aaggaacttt tcttgaagat gctgaaataa ttgaagttga
     5701 ttctatatct aaaagaggta tagacacact tattgaaaaa atagataaaa tgagtgatga
     5761 tatagaagat aaaaatgaaa actctcctgc aagacttaat atagacagag tattttcaat
     5821 aaaagggttt ggtactgtaa taacaggtac tttaatagaa ggaaagataa gtatagaaga
     5881 tgatttggtt atatatccac aggtattaaa aacaaaaata agaagtatac aagttcatgg
     5941 agaaaataaa gatacagctt atgcaggaca aagaacagca atcaatatat caaatgtaaa
     6001 agtggatgat ataaaaagag gggatgtttt agctgcacca aactcattag aggaatcaat
     6061 gatgttggat gtaaaattat cattggtaaa tcatttagac aaaggattaa aacactggga
     6121 caggttgaga ttgtatcatg gaactagaga gatactttgt agggcagttc cactagataa
     6181 agaattaatt gaaagtggag aaagtggcta tgttcagtta agacttgaag aaagcatagt
     6241 atctaagaaa ggcgatacat ttgtagttag aagttattct ccaatggaaa ctataggtgg
     6301 aggtgtaata attgacccat ctcctaaaaa acataagaaa tttgatgaaa aagttataga
     6361 agcattaaaa ataaaagaaa aaggcgaact aaaagatata atagaagagt acttaaaaag
     6421 gaacctaaaa aattatccaa atataaaaga aataatgagc tatagtggtg ctcatgaaga
     6481 agatgtaaaa agagcacttg aaacacttat atcagaagat agagtattta ttataggaaa
     6541 tatgtatatg catataaatc aatacaataa attaaaagaa aatacaataa agcttttaag
     6601 tgaataccat aagaagtata ggcttagaaa agggatatta aaagaagaag taaggtctaa
     6661 gatagagagt aattttaaga ctagggagat ggatatttta ttagaaaaat tatctacaga
     6721 aaatgctata aaaatagaaa ataatatagt atctctatta gattttgagg ttattttaaa
     6781 tgagaagcaa aaagaaatag caaaaaaaat agaaaaaaga ttaaaatcat gtggagtttc
     6841 ttctatactg acaatagatg aagtttctga aggaaatcat aactatgctg aagtattgga
     6901 gtcaatgata ggtaataaag tagagaaatt agatgatttg tatataatgg acaaagatat
     6961 ttatgaaaat gcaaagaaca ttttgataaa ttatataaag gaaaataaag aaataacatt
     7021 aggagaatac agagatttaa ttgattctag cagaaagaat tgcatgatta tattggagaa
     7081 ctttgataga aacaaaataa ctaaaagagt agaaaacaaa agaattttgt tttagcagaa
     7141 aatgtctgtt tttgctttaa ggtataaaga aggaattata atgtcgagta tcgtataatt
     7201 aattagtata aaatattaat taagaggaag tgtaaagtat tgaagaatat aatacctgat
     7261 gtaaaaccag atataaaaga aagagaagac atgagccttt tatttataat aatgggctca
     7321 gtacttttat tatttattat aaaagatata gagaaatatg aaactttatt acatgctata
     7381 tatactatat ttttaatagc tacgggaata attatattta atactacaaa atttaaagta
     7441 gattcatttt ctacattttt aggattaatt tttatagcta ctggagtatt ggagtgtata
     7501 tatttattta attccttggg gattaaaact aagtctataa tggagattaa catcacaata
     7561 tcagcaataa cagatttatt tcctatactg ggtgtatact taagttttaa atttgttaag
     7621 gataacaaac aaatttattc aagtgtaatt ttatttatag caactgctac attaacaata
     7681 acatctttat tcataatatc tggaatttgt gactatgtgg gaaatggtaa atcacaatat
     7741 gctttagggg ttataatatc tatattcata ataattattt ctattattag tggaattgaa
     7801 ttaaattcaa gttcaaataa aggtaaatgg gaatataatg aaaaaaagat ttttaacaga
     7861 ataatagtaa taataatatt atcaagagtt ccaaatctat tgcatatagt tattgataat
     7921 aggaatgtag agagtatatt atcacagata ataataaatg tagccttata ttatttatat
     7981 aattacatag tatctaagaa tattaagaaa acagcactgg aattgcatga tacaaatgaa
     8041 gaattaacaa aaaaaacgga aagtttaaaa gaaaagaata aaaaattaat ctatgagaca
     8101 gaaaagatag aggaacttaa gggaatactt atgaaaagag aatctaggct acaatctaca
     8161 ttagatgtag cagtaaatag catagtagta tttagtaaag atggagaaat tacatatgca
     8221 aataagagct ttagaaatac ttttggagac tataaagaac aagataaatt gactgataaa
     8281 ataaaaaact tcaatgattt gatagaaagc attcattatg tgtttgttca tgagaagaat
     8341 gtagaaaaac ttattagcac aagcgataat aaagtttatc aagctatatt tgcaccttta
     8401 attattgctt cacaaacaga aggagtattg tgtgtactag aagataagac taagaaaaag
     8461 gagtatgaac gcaaacttat tgaagcaaat aagagaagta gagatttttt agaaagtatt
     8521 ggagatggca tagtagtgct tgaaggtaat aacaaaatat atgttaataa tgcatgtagg
     8581 gaaattttta aaaataattt agaaagaatt gatttttcat tagtatgtaa aagtgaagaa
     8641 agtatagaaa aaagatatgt aatagatggt gaagtaaagt atgtagagat gagtttttct
     8701 cagtatacta atgctggtac taataaaact attattgtaa tacgtgatac aacctctcga
     8761 aaaatatcgc aaataaaact tgaggaaagt caatcatcat attcaagatt tatagatata
     8821 ttacctgatg gcatatgctt attaaaagaa gatttaagta taaactatgc aaataagtcg
     8881 tttttagata tgttagcttt tacaaacata gatgatataa aagattctaa tattaaacta
     8941 ttaatgaatg cgaatactga agaaaaaatg aaatttacgg ataatatggc taaagtttta
     9001 aatgaaaata gaagtatact tttactggaa tatgaattga taaatagtta taatgatata
     9061 gtagaagtag aattaagtgc attacctttt gctatataca atactagata tataatgctt
     9121 attataaaag atttagtaca taagaaatat tccgagcaag cggaaaaaga actgcttgaa
     9181 agatttaaaa cagataaaat caaaacggag ttttttgcaa atatgtctca tgaattaaaa
     9241 actccactaa atgttatatc tagttcaaat caactagtag attctttcta tagaagtgaa
     9301 aagataaaag attacaataa caatattaaa tctcatgtgg atttagttag acaaagttct
     9361 tataggcttc aaagattaat aaataacatt atagatttaa caaagatgga gtccgggttc
     9421 tatacactaa aacttgcgaa atacaatatt gtatctgtaa tagaagactt atttatgaat
     9481 atagaagagt atgcacttag aaaagacata aaaattcttt ttgatacaga ccttgaagaa
     9541 attaatgttt atatagacaa ggttgaaatt gagagaatta tgttgaatct attgtcaaac
     9601 tgtattaagt ttactgataa tggaggctgg atatatgtaa gtattcacta taaaatagat
     9661 aaagtaatca taagtgtaaa agacactgga gttggaatac cacaagataa attagaattg
     9721 atttttgaag aattttcaca agttgataag actttatcca gaaatactga aggaagtgga
     9781 atagggcttg ctatagttaa gaatctagta agtcttcatg gtggagatat agaagtagta
     9841 agtgaagtga ataaaggcac agagttctta atttcacttc caatgaaggg atttagtagt
     9901 gaacattaca cagaagataa gagaatatat aatattcaag aaaaaataaa aatagaattt
     9961 tctgatatat actattaaaa atgattatga gcaaaaataa aataaaaata ctatttgttt
    10021 aaatatttaa gttgtaagaa ataggctatc ataaggtaaa ttattatgat agcctatttt
    10081 tagttaggtt ttatatctat atgtgagaac atataatctc taaattacct tcaaaactaa
    10141 aatctccaaa gtcgtttggt agtataaaat gcataccctt ttttaaattg tattctacag
    10201 aattgtgtat aagttttcca tttccatcca acacactgta taaagaaaaa ggtgtattgt
    10261 gtgtaaaatt acatttccca aaaacatcta atttataaac actaaaaaat tcatttgaca
    10321 caaatgttgt gcatttaaag tctgacgttg atacgatttt atagtcagta tcgaaattta
    10381 tatgaggaac attcgtaaca tctattgatt tttgaatgtg aagttcacgc ttattacctg
    10441 aattgtcagt tctatcataa tcatagactc tataagtagt atctgaattt tgttgagttt
    10501 ctaaaattaa tgttcctttg catatagcat gtatagtacc acttggaaca tagaaaaagt
    10561 ctcccttttt tatattaacc tttctaagta gtttgtccca ttcattatta tttaccatat
    10621 ttacaaattc tttatgagat tttgcattat gacctattat aatttctgca tcatcagaac
    10681 aatctattac ataccaacac tctgtcttac ctaactctcc attttcattt tttttagcat
    10741 attcatcatt tggatgaact tgaactgata aattatcatt tgcatccaat atttttgtaa
    10801 gtaaaggaaa tttatctcca ggtgtgtttc caaataattc tctattttta tcccacaatt
    10861 cagatagttt tttaccttta tattttccat tttctattaa gcaatctcca tttttatgag
    10921 aacttatagc ccaacattca ccagttgttg gtgaatctat ttcataatta aatttatctt
    10981 taagtgcagt accaccccat attctatcca taaaaatagg ttttaaaaat aatggttcca
    11041 taatcctgtt cctcctatga tattaaattt atcctacaaa taattatata taaatagatt
    11101 ataaaatcaa tagtaaaaaa gttttgtccc ttgaaatgac ataaagtata aggagttatt
    11161 aaataagata aactatgata tataatataa agttctaaat tggagtgata attatggaat
    11221 tgtttaagac agaaagcaaa aaatatgaaa aagaattttt aaaattattg gaagagtggg
    11281 ttagtatacc ttcattctat gatagaaaaa ctgtatctaa agatatgcct tttggaaaag
    11341 gagtatatga tgcattaaat tggtttgaaa atctaggtag agaaaacaat ttcaaagtca
    11401 aaaatataga taatcatgct gttcaaattg aatatggaaa tggtaaagag tatgtagata
    11461 tatttggaca ttgtgatgtt gtaaatccag gtgaaggatg ggatagtgaa ccatttaaac
    11521 taaatattat aggtgataaa ttagttgcta gaggtgtatc tgataataaa ggacctatga
    11581 tagtgaattt tttagcacta aaaatgataa aggatttaga tattaattta aaaagaaagg
    11641 ttagattaat agctggagga aatgaagaga gtggatttaa gtgtataaag cattattata
    11701 gcaaagagcc atatggtgta tgtggattta ctccagatgc taaatttcct gtattaaatg
    11761 gagaaaaagg tggagcaata ataaagttaa tttcaaatat agacgataaa tctttatata
    11821 taagtggtgg aatagagttt aatacaatac cagataaagt ttatataaag aatatagaaa
    11881 aattgggtaa agataatata tgttttgata ttaacaatat atcgataaat tatgataatg
    11941 gcaattatat tgtgcatgga aaaggtggac attcatctaa acccgaaaaa tcaataaatc
    12001 ctatattagc tacaattaaa cttttgtcag aaaatattga tgaaaaatgg acaaaagatt
    12061 tatataagtt gataaatcaa gataatataa atggaaactt atttggttta aatattgagg
    12121 ggaaatgtgg gattttatca atggtgccaa caataataaa tatagtagat ggaaaattag
    12181 aagtagtttt aagcgttaga tatccagaaa tattaaccat tgaagatata atgaaaaaat
    12241 ttaatttgta tatggagcaa aataatatta ataaatttga gcttattggg gaaaatttaa
    12301 agcaagcaaa ttatatagat agaaattcaa aacttgtaag aagtttgcat gatatatata
    12361 taaagtattc tggagattta aaaaatgatg ttagagtaac tagtgcagga agttatgctt
    12421 ctgaaatgaa taattcagta atatttggtt gtgaatttcc tgatggtagc tttggtaatg
    12481 tccatagtgc aaatgaattt gctagcttag atagacttat agatgcaata ggtatatatg
    12541 cagaggcaat aattacatta tgtaataaaa tataaataca aaatgtccct tatatagaca
    12601 tattatatat acaattaaaa ttttacttga tatataattg aaatcaaaga gaaggtgata
    12661 caatgataac taagaaacat gcagtaatag taaaaagttt aagcaataaa gacggatata
    12721 tgacatcaaa tgaactagct atcaaactgg atgtgtctac taaaactatt aaaagatata
    12781 tagcagattt aaatagtgtt ttaagcaagt atgatttggt aatagcttct tctagaggta
    12841 taggatacaa gttaagcggt tctaaaaaca atatagccag agctgttaaa gaagcaaata
    12901 aatatattga tggtttttta gatgattctg aagagtcaag aatgtcaaat ataatatgta
    12961 tgcttattaa tagaaattat atgagtatag aagcaatggc agaagaatta aatctaagta
    13021 tagccgctat aaataaattg tctagtaagc ttaaaagaaa acttgaaaag tatgacttag
    13081 ttataaaatc taaaccttac tatggaagct atatatatgg tgaagaaatt aatataagac
    13141 aattaattac tgactatgca ataaaattag atgaaaaaag taaagtgaag gtgtttttag
    13201 atgacattag tgaaaacgat atatactgta tagaaagtat tttagaaaag cacctaagaa
    13261 aaaaagatac aataatttca gataaagatt ttaatctttt gctttcaaaa ataatcgttt
    13321 ctgtatttag gagtaagaga ggtcatagta acaatataaa cttaatggat acaagttata
    13381 gatttcataa ttactacttt atagaaagcc taatgaaaga aatatctgat aaaattggat
    13441 ttaagcttat tgaagatgaa gtaatttata tttctaatta ctctggtgta atagcctata
    13501 aaggtacaca aggaagaaaa aacacaagtg aaattgagga acgaataagt agtgttataa
    13561 gtagtgcact tcaagatata ttccttattt cgggtagtga ttatacaaaa gatgaagagt
    13621 ttatggttgc aatatccgac catataaaga gatttttaaa tagagctaaa gctaatataa
    13681 aatcaaataa tcctttactt catcaaataa aagagaagtt tcctatagct tttaatctag
    13741 ccgtgttttt atcaaataaa ctagaagctg agtttaattt aaaacttgat gaagatgagc
    13801 ttggatatat agctatccac tttgctgctt caaatgaaag aatgaaaaaa aatactagta
    13861 agaaaatttg tatagtttgt cattatggga taggaacagg acaacttatt tctgaaaagc
    13921 taaaacagaa tataagtgat ttaagtgttg taggtgttta tccagttaga tatctcgata
    13981 tggctataag tcaagatgtc gatttgatag tatctactgt tgaattaaaa ggatatgaaa
    14041 aacctgtctt atatatagaa aatatatttg atgatagctt aattgaaaac gtaaacaaag
    14101 ccttttatga aaaggaagaa agaagaaaga taataagtaa catgtttgat gaaaaagctt
    14161 tttttagtat aaaggctaac actaaagaag aagttatact ttcactttca aataaattaa
    14221 aagaaagaga ctttatagag gaaagttcta taaaaagtat aatagataga gaaaatattt
    14281 catcaactga aataggaaat ctagtagcaa tacctcacac aatagtgaag ggagataaaa
    14341 aatctataat aggagttgga atacttgaaa atccaataat ctgggataaa caagaggttc
    14401 agctagtatt tatggtattt tttaatacta aagaaaagca taatttctcg atatttaaat
    14461 acctttataa ttttataaaa gatgaaggag gagtaagagg aattattaaa atatgtgact
    14521 ttaataaatt aatggcactg ataggtaatt aaggaggaaa tcaataatgg gaacaaacat
    14581 atttaacaaa gaatatgtat ttttaaatgt tgatgctaag tcaaaggttg aagttttaaa
    14641 atttatatca aaaaaagcta aagacttaaa cttggcagag gatgaaagct tagtatatga
    14701 aggtctaatg gctagagaag aacaatttac tacaaattta ggagaatcta tagcaatacc
    14761 tcatactaaa aatgatgcaa ttgaaaatcc agcagtagta gtattgaagt ttaatgaaga
    14821 tgttgtttgg aatgaaggtg aggataaggt taaattagca ataagcttac ttatgccagg
    14881 aaaaagtaaa gagaatattc atcttaaatt attatcatct ctttcaagaa aattaataaa
    14941 taaagaattt aaagattctc ttttaaaaag tgataatatt gaagaaatat ctaacttaat
    15001 aaatgaagct ttaggtttat aattaaacac taaggttata aaataaaaaa ataattaact
    15061 aaaaatagga ggaatttgaa atgaataaaa aattagtagc attatgtgct tgcccaatgg
    15121 gactagctca cactttcatg gcagcagaag caatagagca agcagcaaag gcattaggtt
    15181 atgaagcaaa agttgaaact caaggtgctg atggtgtaca aaatgagtta acaagagatg
    15241 atatattagg agcaactatg ataatacatg cagtagcaat aacaccagaa ggtatggaaa
    15301 gatttgatgg ttgtgaagtt tatgaagttg aattacaaga agctataaaa aatgcagaag
    15361 gtgttataaa agaaatagaa gaagatttag gaatataata aatttattgt ggaggaaata
    15421 gaatggcaat taagaaaaaa gttataaaca gttcaggtgc ttctacaaca ggaggaaata
    15481 atgtaggaaa atctactcca tctagtccaa ctcaatcaaa gggaaatgga aaatggaaag
    15541 aaataagtaa acatataatg acaggtatat catatatgat accagtttta gtaatgggtg
    15601 gacttatagg ggccttatct caattaatac catatgctat attaggactt gacccatctg
    15661 taggtatagt tgatgctatg aactcaggag aatttacagg attcaagctt agtcttttaa
    15721 atatagcaca attaatgtca aatttcggat ttactttgtt tggatttgca ataccactgt
    15781 ttgcagcatt ttgtgcaaac tctataggtg gaaaaacagc tttaattgca ggatttatag
    15841 gtggatatat agctaataaa cctgtaggtg taccacaatt cgttgatgga caatggactg
    15901 aagttgtgcc agttgcatca ggatttttag gtgcaatatt aatagcattc ataataggat
    15961 attttgtaaa atggttaaat aaatctataa aagtttctca taactggtta gcttttaaaa
    16021 caacattttt aataccacta atagcatcat tagcgtgtat ggtattgatg atatttataa
    16081 taactccatt tggtggatta attaatgaaa gtatgaagaa cttcttaaca gcagcaggag
    16141 cagctggaga atatgtatat gcaacagctt tggcagcagc tacagcattc gacttaggag
    16201 gtccaataaa taaagcagca ggattcgttg cacttggttt aactacagag aatgtacttc
    16261 caataacagc aagaacaata gctatagttg ttcctccaat tggattagga ttaacaacat
    16321 tattagataa aagaatagta ggaagaagag tatatgatag acaattctat caagcaggaa
    16381 aaacttctat attcttatca tttatgggta tatctgaagg agcaatacca tttgcacttg
    16441 agagaccagg ttttgttata cctttaaaca tagtgggaag tgtaatagga gctattacag
    16501 gtataattct aggagcaatt caatggttcc cagagtcagc tatatgggca tggcctttag
    16561 tagacaactt atttggatat ataataggta tagcagttgg agctatattc atagcagtag
    16621 gaaatatatt ctatagaaat aaattaataa aagatggtaa acttgttgta gattatatcg
    16681 actagtattt aatttttact atctataaat aactttggct ggtaatatgg ttttaatcaa
    16741 tattaccagc cttacctata tgattaatta aaggcggtgg agataagata tgaataagat
    16801 agaaaaagta agagagtatt ttatagaata tgatatagat ggatttctta taaatagtcc
    16861 tacaaacaag ttttttgttg gaaatttatt tagttcttca ggttatgttt ttataacgaa
    16921 agaagcacaa tatataatag tagattttag atattttgag gaaattaaaa gaaaatctag
    16981 tttgtttaat gtagtattaa tggacaaaac tagaactcat tttgatataa ttaatgatat
    17041 ttgtagagag cagaatataa aggaaattgg atttgaggga aatgaagtat cttttgattt
    17101 atatagaagt atgagtaata agttaagtgc aaccttaaaa tcagttgatt tatctacatt
    17161 aagggaaata aaaaatgaag acgaaataaa atatataaaa aaagcttgtg aaatagttga
    17221 tgctacattt tatcatataa tagactttat aaaggttggt atgactgaaa agcaagttga
    17281 aaatgaaatt gttagagtta taaaagattt aggtggtcaa aaagaatctt tcgatacaat
    17341 agttgcatct gggcttagag gagcattgcc tcatggaaaa gcaagtgaaa aagttataga
    17401 gtatggagat tttgttactt ttgactttgg tgctaagtat aataattatt gttcagatat
    17461 tactagaacc atttgtatga gtactataaa taaagagtta gaagaaatat ataatatagt
    17521 aagaaaagct aatgaagagt gtataagggt tttaagacca ggaatgacta ctggagagat
    17581 agataaagtt gcaagagata taattggttc atatggatat gccgataatt ttggtcataa
    17641 tctgggtcat ggtgtaggaa ttatggttca tgaatatcct gcattagctc ctgaatcaaa
    17701 tgaagtatta aaagaaggaa tgattgtaac tatagagcct ggtatatatg ttccaagtct
    17761 tggaggcgta agaatagaag atgatgtact tataacacaa gatggatgta tgagattgac
    17821 tacatctact aaggatttaa tagttgtcag ttaaaaataa attaggtaaa tattttataa
    17881 caataagaaa agaagaggta aatttaaatg tataattttg atgataaacc agatagagta
    17941 agtgaaaagt gtagaaaatg ggatttaaat attataagag ataaatttgg agatattaga
    18001 gaagattttg taccaatgtg gattgctgat atggatttta aaataccaac agaaatagaa
    18061 aacaaattta ttgaagctgt aagaagaggt gtatttggat atacatactg ttatgatgaa
    18121 ttttatgatt cagttattgg ttggcaaaaa aatatgcatg aggtacatgt agaaaaagaa
    18181 tggattacat taacttatgg aactgtatca acacttcatt atgtagtaca agctttttgt
    18241 aagaaaggtg atagtataat attaaacaca ccagtttatg acccctttga atcatcagca
    18301 aaaaaacagg gtgtaaatgt aatttgtaat acattagatg ttgtaaatga tagatattat
    18361 ataaattttg ataaattaga agttcaaatt aaagaaaata agcctaaatt aatgatgttt
    18421 tgtacaccac ataatccatc tggaaggata tggacaattg aagaaatgac aagagtagca
    18481 actatatgta aagaaaataa tgttattttg gttgcagatg aagtacatgc agagcatatt
    18541 cattatggta aatttaactc aatacttaag attgaaaagg agcttttaga aaatgttatt
    18601 ctattgacat caccaaataa aggatttaat cttggtggac taaagacttc ttattcaata
    18661 gttataaata aggatataag ggacaaattt agaaataaac taaagcaaaa ttctataact
    18721 tctccaaatg tgtttggaat aataggattg ataactgctt ataatgaatg tcatgagtgg
    18781 ttaagtggtg taaatgaata tataaaatca aattatgaac ttttagaaac ttgggtaaat
    18841 aaatataata agataaaatt gatgaaaatg gaatcatctt acttagcttg gatgaatata
    18901 agtggattag gtataagtgc aagtgagttt acagataggt tggctataga tacaggggta
    18961 ttattagaag atggaagcca ttttgtaaaa gatggtgaga agtatgttag aataaactta
    19021 ggaactcaaa aggaaaatgt aattgaagct ttaaatagaa tggatttatt tttaaagtct
    19081 ttataaaaat ttggttgaat tagtatttta tataaattta aactattaat atggtatata
    19141 cat
//
LOCUS       DAEMAE010000057        18915 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEMAE010000057 DAEMAE010000000
VERSION     DAEMAE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.058"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="KPH93_001592"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="KPH93_001592"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            313..504
                     /locus_tag="KPH93_001593"
     CDS             313..504
                     /locus_tag="KPH93_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG1688420.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            659..2068
                     /locus_tag="KPH93_001594"
     CDS             659..2068
                     /locus_tag="KPH93_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1688421.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQIYKKLGYKDILENLYKM
                     DTTEILGTDNLHFPEGIIKNSQEKASRVFKSDYTCYLVNGSSCGIQSAIMSVCRPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEVSEETNVLKGISISNVIDIVDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPSFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRTFKNNVDSNINIYDTNDKTKIFISSKNMGLTGYELESILRNKYNIQV
                     ELSNYYGVLLICTIGNTAKDFSSLETALNNVIKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2189..2878
                     /locus_tag="KPH93_001595"
     CDS             2189..2878
                     /locus_tag="KPH93_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG1688422.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPKPDCVIFLDVPIEFSEKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKSMER"
     gene            2881..3816
                     /locus_tag="KPH93_001596"
     CDS             2881..3816
                     /locus_tag="KPH93_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG1688423.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIIILKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3813..4706
                     /locus_tag="KPH93_001597"
     CDS             3813..4706
                     /locus_tag="KPH93_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG1688424.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4784..5530
                     /locus_tag="KPH93_001598"
     CDS             4784..5530
                     /locus_tag="KPH93_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG1688425.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSVKLNGLEERVKIINED
                     IKYIDKVLDVNGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5531..6364
                     /gene="rsmI"
                     /locus_tag="KPH93_001599"
     CDS             5531..6364
                     /gene="rsmI"
                     /locus_tag="KPH93_001599"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG1688426.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHKFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6387..7091
                     /locus_tag="KPH93_001600"
     CDS             6387..7091
                     /locus_tag="KPH93_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG1688427.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYTEKGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7279..7707)
                     /locus_tag="KPH93_001601"
     CDS             complement(7279..7707)
                     /locus_tag="KPH93_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG1688428.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7744..9174)
                     /locus_tag="KPH93_001602"
     CDS             complement(7744..9174)
                     /locus_tag="KPH93_001602"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG1688429.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9306..9890
                     /locus_tag="KPH93_001603"
     CDS             9306..9890
                     /locus_tag="KPH93_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG1688430.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9906..10427
                     /locus_tag="KPH93_001604"
     CDS             9906..10427
                     /locus_tag="KPH93_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG1688431.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMLSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10493..10739
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10823..12760
                     /gene="metG"
                     /locus_tag="KPH93_001605"
     CDS             10823..12760
                     /gene="metG"
                     /locus_tag="KPH93_001605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG1688432.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFRKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12790..13560
                     /locus_tag="KPH93_001606"
     CDS             12790..13560
                     /locus_tag="KPH93_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG1688433.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRATKENAKKFFNIK"
     gene            13904..14335
                     /gene="phnG"
                     /locus_tag="KPH93_001607"
     CDS             13904..14335
                     /gene="phnG"
                     /locus_tag="KPH93_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG1688434.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNTMD"
     gene            14350..14925
                     /gene="phnH"
                     /locus_tag="KPH93_001608"
     CDS             14350..14925
                     /gene="phnH"
                     /locus_tag="KPH93_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG1688435.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQEDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGVREEFIERLSTINK
                     DYPIGIDLILVDKKGELAFIPRSSKLSWEVL"
     gene            14928..16013
                     /locus_tag="KPH93_001609"
     CDS             14928..16013
                     /locus_tag="KPH93_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG1688436.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVDLLEEDEKKNRDFKDSFINELETVDEIPE
                     FTKISNIFRNENLLQEEVCENKSEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16033..16902
                     /locus_tag="KPH93_001610"
     CDS             16033..16902
                     /locus_tag="KPH93_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG1688437.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAVSIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRNFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16895..17761
                     /locus_tag="KPH93_001611"
     CDS             16895..17761
                     /locus_tag="KPH93_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688438.1"
                     /translation="MSKINDSKRLESIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILKLSDPKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17802..18515
                     /locus_tag="KPH93_001612"
     CDS             17802..18515
                     /locus_tag="KPH93_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1688439.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18516..>18915
                     /locus_tag="KPH93_001613"
     CDS             18516..>18915
                     /locus_tag="KPH93_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG1688440.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYIIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNI"
BASE COUNT         7576 a         2107 c         3393 g         5839 t
ORIGIN      
        1 taactctaaa gaaactagtg ttactagatt ctaaaaaatt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgtaatttt tttatttttt gtcaaaaaaa acaaaaaata cgtatttctt
      241 ttttcatttc gggaaaacta aacacaggaa ttatggactt tataaaaatt taattcttga
      301 ggagtgatat aagtgaaaaa agttgcttta attttagcta taataggtgc acttaactgg
      361 ggtgcaattg gtattctagg tacagactta ataggtagta tctttggtgg aacatatgaa
      421 atggtaagta gaataatata cttcattgta ggtttagctg gattatactt aataccaagt
      481 ttaatgtctg atgatagaga ataatttatg cttttatgtt aaatacctca ttctaaatgt
      541 ttagaatgag gtatttaatt ttataaaaat attaatttgt ttctttttta atggtaaaat
      601 agataagaat tgaagtttta aaaagaagta ttatgtaaaa aaggagtact attaatatat
      661 gtttattatt aatgaattaa ataagattgt aaatgaagaa gtaatatctt ttcatatgcc
      721 tggtcataaa aagggacaaa tttataaaaa gttaggatat aaagatattc tagaaaattt
      781 gtataaaatg gatacaactg agatattagg aacagataat ttacactttc cagaaggcat
      841 tataaaaaat tcacaagaaa aagcatcaag agtatttaaa agtgattata cttgctattt
      901 agtaaatggt agtagctgtg gaatacaatc tgcaataatg tcagtatgta gacctaaaag
      961 taaaataata acaaatagag attgtcatca gtctgttata aatggatgta ttttaggaga
     1021 tatagatata gagtatgtgc cttgtgaggt aagtgaagag acaaatgtct taaaagggat
     1081 atctataagt aatgtgattg atatagtaga taaaaacttg gatgcaaaag cagtactgct
     1141 tacttatcct acatattatg gaatgacata tgatttagaa tacatatgta attatgcaca
     1201 tagcaaaaag atggtagtga tagtagatga agcacatggt gcgcatttag ggcttagtga
     1261 gaggcttcct aaaacagctc ttgaacaagg tgcagatata gttgtacaga gtacacataa
     1321 aactcttcca tcatttacac aatcgtcaat gatacatata cagggagacc gagttgatat
     1381 agataaaata ttgaacatgt taagaattac agaatcttca agcccttcat atttattatt
     1441 atcttctctg gaattagcag tagatatata tgaaaataaa ggaaaagaat taatggaaga
     1501 gcttttaaat aatattagaa ctttcaaaaa taatgttgat tcaaatatca atatatatga
     1561 cacaaatgat aaaacaaaaa tatttatatc atctaaaaat atgggtttaa caggttatga
     1621 actagaaagt attttaagaa acaaatataa catacaagtg gaattatcta attattatgg
     1681 agttctttta atatgtacaa taggaaatac tgctaaggat ttctctagtt tagaaacagc
     1741 attaaacaat gttataaagg aagactttaa atttgaaaag ttagaaaata aaaaataccc
     1801 tgttgttatt ccaaagaaaa tattaacacc tagagaagca ttttataaaa ctaaaaaaag
     1861 tgttaaaata tatgacagca taggtaaaat ttgtggtgaa tgtatagtgc catacccacc
     1921 tggaataagt gtaatatctc ctggtgaagt aataagtaaa gaaataatag actatctaat
     1981 gttttgtcat tctaaaggta tgattgtgag tggattgaaa gatgtagatt taaattttat
     2041 acaagtaata gatttagaag acatttaaaa attatgatat aaaagtagat agtttgaaat
     2101 tgtgaagaga aataaataga ctgcttatat agagctatct aataaaagta ctaaaaaagt
     2161 aaaaagtgtt tgtatggagg atatgaaaat gaaaggtaag ctaataatta tagagagtgg
     2221 ttcagatgca agtggaaagg ctactcagac agaaatatta tatgaaagat taaaaaaaga
     2281 aggctatatg attaaaaagg tagaatatcc aaattatgaa tctccatcat ctgccttggt
     2341 gaaaatgtac ctaaaaggag actttggtaa aaatgcatca gatgtagacc catatatagc
     2401 atcaactttt tttacagctg ataggtatgc atcatttaaa accgattggg aggagtttta
     2461 caatgaaggt ggaataatta tcgcagatag atatacaacc tctaatatgg tacatcaggc
     2521 ctctaaaatg gatatttgtg atagagaaga gtatttaaat tggttagctg attatgaatt
     2581 taatttattt aagataccaa aacctgattg tgttatattt ttagatgtgc ctattgaatt
     2641 tagtgaaaag cttatggaga atagaaaaaa taaaattaca ggggaagaaa aaaaggatat
     2701 tcatgaaagt gacatagagt atttagagaa atcttataac aatgctttat atatagcaga
     2761 taaatatgat tggaaaaaaa taaattgtgt tttagataac aaattaagaa gtattgaaag
     2821 tatacataat gaaatttata aaatagttct tgattcaata aaatctatgg agagataaat
     2881 atgtattttg aaaatattat agggcaagat tttgcaaaga aatatttaac aaattctata
     2941 aagaaaaata aacttaataa tgcgtatatg tttgaaggaa tggatggaat aggaaaaaag
     3001 aaatttgcag atgaattatc aaaactatta cttgattatg aaaatcttga aaatagtcca
     3061 gactatgtac tcataaaacc agatggaaat agcataaaaa tagctcaaat cagaaattta
     3121 caatcagata tagtaataag acctcataaa gactataaaa tatatataat caataatgct
     3181 gaaaaaatga ctgtagaagc ccaaaatgca cttttaaaga cgctagagga acctccaaac
     3241 tatgctataa taatcttagt cacaaacaac aaggaatctt tattagaaac tataaaatct
     3301 agatgtgata taataaagtt ctcgcctata ccaattgagg acttaaaacg ttatttaata
     3361 aatacaggta tagaagaaga aagagctcaa ttattggcta ttttttcgag aggaagtatt
     3421 gagaatgctc taaatctatc tcagtcctct gagttttcaa tgatgagaga agatatacaa
     3481 caatatattc agataatgtt agataaaaac atagtagaga ttttaaatat accaaataat
     3541 atggaaaaat atagaggtaa gattatagct ttattagata tgatgataaa ctattttcgg
     3601 gatatcatca tattaaaaga aaatgtaaac aagaacatgt taataaatgt agataagcta
     3661 gttttcattc aaaatatgag tggaaaaatt agttattctc aattgtccaa gattattgat
     3721 ataatagaag atgcaaagag caaaataaaa agcaattgca atttcaatat aagcatacaa
     3781 gttatgtctt taaatatata cgaggtgatt aaatgataaa gatagtaggt ataaggttta
     3841 aaagtgctgg gaaaatatat tattttgacc ctgttgattt taatatagag caagatatgg
     3901 atgtagttgt agaaactgca agaggcttgg agtatgggaa agtagtcgtt ggtccaaaag
     3961 atatggatga aagcgaatta gtttctcctt tgaagccaat tataagaata gctacagaag
     4021 aagataaaaa agtatatcta gaaaataagg aaaaggcaaa agaaactttt gagttatgcc
     4081 aacaaaaaat aaaagaacat gagttaacaa tgtttttaat agattgtgag tatacgtttg
     4141 ataggaataa gttgattttt tactttacag cagaaggaag aatagatttt agagaattgg
     4201 taaaagactt agctgctata tttaaaacta gaatagaact tagacaaata ggtgtcagag
     4261 atgaagctaa atctatagga ggtctagggc cttgtggtag aaaactttgt tgttcatctt
     4321 ggttaggtga ttttcaacca gtttcaataa aaatggcaaa agaccaaagt ctatctctaa
     4381 atccaacaaa aatatctgga atatgtggga gattattctg ttgtttaaaa tatgagcatg
     4441 atgtatatag tgaggcaata gaaaaaatgc ctgttgtagg tgcattggtg caatcagatg
     4501 atggaaaagg aaaagttgta gaagtaaatc ctttattaga acaaattaaa gtggaatttc
     4561 aagacaagac agtaaagata tacacaagag aagatataaa aatacttaga gagcctaaaa
     4621 aatgtgatgg ctgtggtggt aaatgtaagg ataatgatgg attagatgaa gccacactaa
     4681 gagagctaaa gaaattagaa gattaaattt aaggtagggc ttttcctacc ttttttaaag
     4741 caattaatgc ttaaagttat taatatttga ggagaattta tatatgcaat taaaagattc
     4801 agaaagaata gatgatttac aattaaaagg tcttaaaatt atacaagata ctaatggttt
     4861 ttgttttggt atagacgcag ttttattagc taattttact aaaataaaaa aagatgctaa
     4921 agtagtagat ttgggtactg gaactggtat tatacctatt ttgatagctg gaaaaagtga
     4981 agctaaaaaa attattggtg tagaaataca agaagatgta tatgaaatgg caactcgttc
     5041 tgtaaaatta aatggattag aagaaagagt taagattata aatgaagata taaaatacat
     5101 agacaaagtc ttagatgtaa atggctacca tgtagtcaca tcaaatcctc catatatgca
     5161 tattgacgga ataaaaaatc caaatgataa aaaagcaata tcaagacatg aagtaaggtg
     5221 taatttggaa gatgtaatta gagctgcatc cagattggtt atgccgagag gaaaattctt
     5281 catgatacat agaccaacaa gattggttga tataataact ttagggagaa aatataaatt
     5341 agagccaaag gttattcaat ttgtgcatcc aagacctcaa aaagcaccga acttagtttt
     5401 ggttcaattt gtaaaagatg gaagaccaga gcttaaaata ttggacccat tgtatgttta
     5461 tggagaagat gggaattata caaaagaatt aaaagcaata tataataata aagatatagg
     5521 agagttgtaa atgagtggga aattgtacat atgtccaaca ccaataggaa acctagaaga
     5581 tataacatat agaactttaa gaatattaaa agaagtagac ttgattgcag cagaagatac
     5641 aagacatagt ataaaacttt taaatcattt tgaaatctca aaaccactaa ctagttatca
     5701 tgaacataat aaggattcaa agggagatta tttgataaat aaactaattg atggtgaaaa
     5761 tatagctctg ataagtgatg caggtatgcc tggtatatct gacccaggag aagagataat
     5821 aaaacaagct atacaaaata acatagagat agaagtattg ccaggtgcaa cagcatttgt
     5881 aacggcttta gttggctctg gtatggacac acataaattt gtttttgagg gctttttaga
     5941 tagagataaa aaagttagga aagcacaatt ggaagaatta aaggaagaaa gtagaactat
     6001 aattttctat gaatcaccac atagattgaa ggatacacta aaagatatgc ttaaaattct
     6061 tggtaatagg aatatttcta taaatagaga acttacaaaa aaatatcaag aaataataag
     6121 agaaaatata gaaactacca taaaaatatt tgaggaaaaa gaagtaagag gagagtttgt
     6181 attaatagta gagggttttc atggtgagaa gtcaaagaaa gaaaattatg atgggcttac
     6241 agatagggaa tatgttttaa aactaattga aagtggtatg aacaaaaaag atgcaataaa
     6301 aatagtttgt aaagatagaa agttaaagaa agatgtggtt tataaacaag ttttagattt
     6361 ataaaacctg aatctgaggt gattttatgg aaataggaaa aattataata gagagtttaa
     6421 agagggaaga tacacataaa atatttatag aattagataa taagcatgaa ttagaaaaaa
     6481 taattcctaa aataagctgt atgaagtctg taggtgaatg taaatatcat gtagtaaatt
     6541 gttttgaaca ttctataaat gcattaaaag aattagaaat agtattaaat gataaggatt
     6601 tttttccaat acatctaaga aaacatgtgt caaactattt aaacacatat actgaaaaag
     6661 gaataaataa gttacatgta ttaaagctgg gaacattttt acatgatata ggtaagcctg
     6721 acagtatgac tttagatgaa acaggaagag ttcacttcac aaatcatgaa aaaataggtg
     6781 ctcaaataat tgacaatatg gggataaagt tggagttatc aacagagaca tatagactta
     6841 taagtaaata tgtaagatat catatgatac ttttgtctct gtataagaaa aatgatttga
     6901 gtaggaaaga attaattaat gtatttaact tggtagatga ggacactata ggtgtaataa
     6961 tgcttgggta tgcagatata gtgtctacag taaaattatt gggcaagagt ggagaagtga
     7021 gtgtattaaa gacatatatg gaatatattt taacaaatta tttgtataag agtaattata
     7081 cacatgtata aatttagtta atataattga gttttagatg attaattaaa gtctgaaata
     7141 cacggatttc aaaaacataa aagaagttgt ctttgttgta aaattatgaa agacaacttt
     7201 ttttatatca aaaattgaaa ttttattaag gatacttaat ggtctattaa ttaataaacc
     7261 attagtattt aatttatatt acaatattga ttttgcttgt attggcgttg atagtataac
     7321 agaggtctta gtagaaccaa ttttctttat agtatcaatc acattttcta gctcgtacat
     7381 atcttttaca attactttta aaagtaagca atcatcacct gttatatggt gacattctac
     7441 aatcctaggg tcctttgcag ctgactcaat aaattctgta tatccattgc ttggaagaga
     7501 aatatgaata aatgccttta taactctgcc taatgaatct gggttgacaa tagctttata
     7561 tccttctata acaccagact cttctaatct tttgactctt tctgaaactg caggagaagt
     7621 taaaccaact atttttccta agtccttcat agaaattctc ccatcatcct gtaaaatttc
     7681 tatgattctg taatctgtaa catccataaa taaacagtcc cccttaaata aaaataatta
     7741 atgctataaa acttttttag aatctatcat ttgtgttttt aaataccaaa gatttctaga
     7801 taaatcaacc ttataaatgt gtggttttga aagtcttttg tattgttccc agatagtact
     7861 taattcatat tgtacatgat tttttatttc aagaacagct tttttattat atttgcatct
     7921 gccttttata tacaatggtt taagaagctc tttaactttg taatttgtaa atacctttgt
     7981 tttccatgta taagtaggat ggaagatttc taaaggttta gactcatcta taacttcatc
     8041 atgtaacata atcaaatctg cctctgcctt attgtcttcg ttatatattc ttataacctt
     8101 cttgtaacca ggattattta ttttttctgg attctcagaa atctttatct taggttcaag
     8161 aactcctttt tcacaagatg cagctaattt gtaaactcct ccaagagaag gtgaatcgaa
     8221 agatgtaatg agctttgtac ctactcccca tgaatttata gtagctcctt ctgctttaag
     8281 tgatgttatg gtatattcgt ctaggtcatt agatgcagtg atgctgatat ctgaaaagcc
     8341 agcgttatct aattgttttt tagcttcttt agataaataa gctaaatctc cagagtcaag
     8401 tcttataccc ataggtttgt atcctttttc agaaatattt ttgaatactt ttattgcatt
     8461 aggaacccca ctgtttaata catcataagt atctacaagt agtagacatt tatctggata
     8521 aacatcagca tatgcttgga atgcttcaag ttcattatca aatttttgta cccaactgtg
     8581 agcttgtgta ccaattatag gtatatcaaa cattttccct gctagtacgt ttgcagttcc
     8641 agcacaacca ccaactacag cagccctagc tccatatatc cctgcatctg gaccttgtgc
     8701 acggcgtaat ccaaattcaa atacagggtc tccttgagct gcaaagcaaa ctctagaggc
     8761 tttagtagca ataagggact gaaaattaac tattgttaac aatgctgttt caataagttg
     8821 agcttgatat aaaggtgcct taacagtaat taatggctca ttagggaaca tgatagttcc
     8881 ttcctcaaca gcatatatat ctcctgtgaa tttaaattct tttaaaaatt ttaagaactt
     8941 gtcagaaaat aaatttaagt tttttaagta ctctaaatct tcatctgaaa aatgaagatt
     9001 atctatgtat tcaacaacct gatcaatacc acaaactata gtataccctc catcacaagc
     9061 gttttttcta aaaaacatat caaatacaac aatgtcttca tgaatattct tttcaaaata
     9121 gccatttaac atagtaagct gatataaatc agtaagaagt gttagatttc tcattatttc
     9181 tctccttaat tattattaaa tattaaaaat tgtaaaaact cccacactta tactaaaaca
     9241 taatatagta caactttcaa gtataaagta ttacctaact atcataaaat tatttaaaga
     9301 ggaggatgat tataatagga aaaatatggt tttatatggt atcgatagga ataataggaa
     9361 gcttgatttc taaaaatatg ggagaattaa ataaagttat attaagtgaa acatcaaaag
     9421 gcatagaatt tgcaataagt ctagctggta tcatggcact ttggatgggg ataatgaata
     9481 tcgcaaaaga gtcaggctta attgaaaaga taggtcaaaa attaaatcct ataatgagaa
     9541 gattatttcc ttctattcca aagggtcata aagcaatgtc ttatatagtt atgaatattg
     9601 ctttaaatat gttgggtgct ggaaatggtg caacagcatt tggtttaaaa gcaatgagtg
     9661 aacttcaaac tttgaataac aagaaagata cagccactaa tgatatgatt atgtttatgg
     9721 taatcaatat atcatctata caaataattc catttactat aatcaaatta aggatggata
     9781 tgggggctca aaatccaagt gaaataattt ttacgacttt atttgcaacc ataatatcta
     9841 cagcagtagc aataataaca tgtaaatttt ttcaaagaag atatagataa aggggatgta
     9901 gatacatgga gatattttca aacttactta taccaataat aattctgtac atagtagttt
     9961 atggtaaata taaaaaaata gatgtgtatg atagttttgt aaaaggagcg attgatggat
    10021 taaaagcagc atgggatatc ctaccatata taataggtat atttttggca ataggaatat
    10081 tcaaaactgg taaaggtctt gatatgttag aatggttatt tacaccaata gcaaatatgt
    10141 tgagtatacc aaaagaatta ataggtctaa taagtgtaaa acctttatct ggaagtggtg
    10201 ctttagggat gtacagtgaa cttgcaaata gagttggaat aggaagttta gtagaaaaaa
    10261 tgggtgccac gattgtaggt tcttctgaga ctatttttta cacaatggca atatactatg
    10321 gaagtcttaa gataaaaaat actagacata cattatcatg tgctatgatt agccatgttg
    10381 caggtgtcat agcagctgtt tttatttgct atgtaatatt tgtataattt gttgacaata
    10441 tgggaaaaat tttataatat agtgattata tagaaacaaa tagtaataca aattataagc
    10501 tatgaaaaga agagtaaata aaggtttttt ctatcagaga gctaggttag gtgaaagcta
    10561 gtgtcaaaag ctttattgaa gatggtcttg gagcttttta tctgagcaag tgtgctaggg
    10621 taaaacggtt ggacacgtta tagtctacta aggtatctat aaaatgtttc tacatttcat
    10681 gtgaaacata actattatag atgaattagg gtggtaccgt gaataatctc gcccctatgt
    10741 taactcatag gaggcgattt tttattattt aaatgaaaga atcatatgat tacgaacgat
    10801 aaaaaattag gaggtaaata acatgagcaa accgagtttt tatgtaacaa caccaatata
    10861 ctatccaagt ggaggattac acataggaca tacttattca acagtagccg cagatacaat
    10921 agctagattc aagcgtttct gtggatatga tgtaaagttc ttaacaggaa cagatgagca
    10981 tggtgaaaaa attcaaaaga aagccataga acaaggaatg tcagagatag agtatcttga
    11041 tggaatgata aaagatataa aggccttatg gaatactatg gatatatctt atgatgattt
    11101 tataagaact acagaaaaaa gacatacaga cataatacaa aagatattta ctaaactata
    11161 tgagcaaggt gatatatata aaggtgaata tgaaggtaga tactgtactc cttgtgagag
    11221 tttctggaca gaatctcaat tactagaagg aaataaatgc cctgactgtg ggagagaaac
    11281 atatttagta aaagaagaat cttatttctt tagattatct aaatatgaag atagattgaa
    11341 agagttattt aaagatgata gtttctgttt tcctgctgct agaaaaaatg aaatggtagc
    11401 aaacttttta gacaaaggat tagaagattt aagcgtaact agaacgactt ttgactgggg
    11461 tataaaagtt ccttttgatg aaaaacatgt aatttatgtg tgggtagatg ctttatgtaa
    11521 ctatataaca gcattaggtt atatgactga taatgatgaa gaatttagaa aatattggcc
    11581 tgccaatgtt caaatagtag gtaaagaaat agtgagattc catacaataa tatggccagc
    11641 acttttaatg gctttaggtc tagaagttcc aaaacaagta tttggacatg gatggatact
    11701 gtttgctgat gataaaatga gtaaatcaaa gggtaatgtg gtttatccag aacctataat
    11761 agaaagatat ggaatagaca cacttaagta cttcttatta agagagtttg catttgggca
    11821 agatggaagt tatactcata gaaattttgt aacaagaata aattatgatt tagcaaatga
    11881 tttaggaaac ttaataagta gaactgtagc tatggttgaa aagtacaata atggtataat
    11941 accaacagca aaagtttcaa ctgattttga tgctgactta aaagagcaag ccgtttcaac
    12001 tagagaaaac tttgaagctg aaatggataa aatgcaattc catgaagcat tagagagtgt
    12061 atggaaatta gttagaagaa ctaacaaata tatagatgaa actatgcctt gggcactagc
    12121 taaagatgaa acgaagaaag atgaattaga cacagtttta tataatttat gtgaatctat
    12181 aagaataatc gcaacactaa taaatcctat aatgaatgag actgctaata aaatatatga
    12241 acatatagga ataaaaggtc aagatgatat aactacatgg gaaagtacaa aaacatttgg
    12301 tcttattgga gaaaatgtaa aagtatttaa aggtgaacct ttattcccaa gattagatgt
    12361 agaaaaagag atagaagaat taactaaaat gttctctggg aaacctccag tagaagaaaa
    12421 acctttagag cataaagaag aaattactat tgatgattta gacaaaatag agcttagagt
    12481 agggaaaata ataagctgtg aaaaacatcc aaaagcaaat aaattattag tatcacaagt
    12541 taagattgga ccagaaacaa gacaaatagt atcaggaatc gctgaatact ataagccaga
    12601 ggatttagta ggaaaagagg ttacagtggt atgtaactta aaaccagtta aattaagagg
    12661 tgtagaatct caaggtatga tattagcagc aggtgatgat ggagacccat atgttttacc
    12721 atttacacaa ggggctaaag atggatgcga ggttcgttag taggtaatta aatagtaagg
    12781 agattaaaga tgttatttga ctcacatgca catttaaatg atgaaagttt tgatgaagat
    12841 agagatgaac ttattggttc attaaaagat aaaggtgtag atttggttgt aaatccagga
    12901 gcagatatag agacgtcgat aacagcaatt gaacttgcga aaaaatacga ctttatatat
    12961 tcagcagttg gggtacatcc acatgatgta tcaaaactag atgatacagc aatagaaact
    13021 ctaagaaaac ttgcaactga gaatgaaaaa gttgtagcta ttggagagat tggattagat
    13081 tattattatg attattctcc aagagaagaa caaaaagagt ggtttaagaa gcaaattgaa
    13141 ttagctaatg agctaaaact tccaataata atacatgata gagatgcaca tggagatact
    13201 tttgagataa taaagaatac taaaaatcct gaaataggat gtgtacttca ttgttatagt
    13261 gggaatgtag agttagctag agaatatgtt aagatgggtt gttatatatc aattccaggc
    13321 acggttactt ttaagaataa taaaaaaact agagaagtag taagagaaat acctctagaa
    13381 agattattca tagagactga ttcaccatat atgtcaccag agccacatag aggaaagaga
    13441 aacaatcctt ctcaagtatc ttttgttgct gataagatag ctcaggaaaa agggatatct
    13501 tatgaagaag tatgtagagc tacaaaagaa aatgcaaaga aattttttaa tataaaatag
    13561 ataaattaaa tcttataata atatatataa taagtaatta taagtatagt atgagagtgc
    13621 ctcaaaatga gttttttagt tcatgaggca ctcttttttg tatgaaatca aatatatttt
    13681 catcatttca acaatgaaaa agaagtttat catattttaa gacagtctat ttttgttttt
    13741 atttatttaa ttcatatttt aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa
    13801 gatattcatt agaattaact caactctaat attcatttaa cttaggttgt ttattatatt
    13861 attaaagaga agtaaacgaa aggtagatta aggaggctaa attttgtata cagttgatag
    13921 tactaagata ttgataaata tgaacttaga gaaactagag aagatatact caatgataaa
    13981 agatacatca agtatttcta taattaaaga acctaactta gctacagtaa tggtaagagc
    14041 taatgaaagt gtaaaaaaca caacatttaa tttaggagag atattagtta ctgaatgtag
    14101 tgtaaaggta gatgaaagct tgggttacgg aatagtttca gaaaacaata ataagaaagc
    14161 catttactta gcagttatag atgcagtttt acatagcaat aatagtaaat ttgatgagtt
    14221 aaagaattac ataaataaaa gtgtatatga agagaactta aggtatgaac aagagattat
    14281 tgatgaattt agcttgatta ataaaacgaa agttcaattc aatactatgg attaaatagg
    14341 aggagaatca tggtaaatta tacacaagaa atatatagaa agatgctaga tagtgcttca
    14401 tttcctggga aattaaataa tatagataat ataaatatag aaaataatac aaaactattg
    14461 aatggagcaa ttggtatagc aatcacttta ttagaccaag aagttacctt ctatatagaa
    14521 aattatactc aagaagatat aaaaaatata agagcactta caatgtcaaa tcaaatagat
    14581 tatagaaaat cagattatat atttttagat attaattcag aagtggatat attacagatt
    14641 aaaataggtt cttttgaata tcctgatgaa agtgcaacta tagtacatca agtaaatgat
    14701 ttatcaacgt actctcaaac taagtatata aaacttgatt tgagtggtcc tggtataaaa
    14761 tctaaaaata gtatatatat aaatggagtt agagaagaat ttatagaaag attatctacc
    14821 ataaataaag attatccaat aggaatagat ttgattttag tagacaaaaa aggagaactt
    14881 gcatttatac caaggtcatc aaaattaagt tgggaggtat tgtagaaatg gcttatgtag
    14941 cagttaaagg tggagaagaa gccatattac aggctaaaaa tatacttgag ttttataggg
    15001 taaaaggaga tagcttgcct atagatgtaa agcagattga ggaacaaatg tcttatgctg
    15061 tagacaaagt aatgtctgaa ggaagtttat acaacaagga actatcagca ttggctataa
    15121 aacaagctca aggtgatata ttagaagcct ctttctatgt aagagcttta aggtcaacaa
    15181 tgcctagaat tacatattca gaacctataa atacagaaga gatgagaata aaaagaagaa
    15241 tatcagctac ttttaaagat ataccaggag gtcagtatct tggggcaact agagactatg
    15301 aaaatagaat attaaatgta gacttattag aagaagatga aaaaaagaat agagacttta
    15361 aagatagttt tataaatgaa ttagaaacag tagatgaaat ccctgaattt acaaaaatat
    15421 ctaatatatt tagaaacgaa aatttattac aagaggaagt ttgtgaaaac aaatcggaag
    15481 aagttattga tgatattaca agaaatagtt taagatttcc agtttctagg tcagcaaagt
    15541 tacaggcatt aactagaggt gaaagtggag tagtgtcagg ttttgcttat gctattgtaa
    15601 gaggttttgg tgatgaacat ccatatataa gtgaacttag aactggtaat gtatttataa
    15661 aagtaaaaca tcccatctat gaagatgaag aaataataat aggtgatatg ctggttactg
    15721 aatgtgaaat agtttcaaaa gcatcacaaa ataagactga aaattctaaa actaattcag
    15781 aagataaaaa taagatgtct ttaggatatg gtctttgtat ggggaataat gagaataagg
    15841 ctatatctat ggccatacta gacaaatctt tggagccaaa tagcgaaaaa ctagcacaag
    15901 atgaagaatt tgtattgctt catatagatg gaatagattc attaggattt atatctcact
    15961 ttaagcttcc tcactatgta gattttaaag cggatgtaga aagaataaaa tagtatatgg
    16021 aggtaagttt aggtgatata caactatggt tttttagatg aaaatacaaa gaaggaaata
    16081 agaagaaaaa tattaaaagc agtttcaata cctggttatc aagttccctt tggctcaaga
    16141 gaattgccta tagctaaagg atggggtact ggagggttac aattaacact atcattaatt
    16201 ggtaaaaatg atgtagtaaa agtaattgac cagggaagtg atgattctac aaatgcttgt
    16261 aatataagga actttattgc aagtgtaagt aatgtagaaa cgacaaaaga tacgttagaa
    16321 gctacattga ttcaaacgag acatagaata ccagaagaaa aacttaaatc taatcaaata
    16381 ctaatatttc aagtaccaat accagaaacc cttagaatag tggaacctag tgaagtagaa
    16441 acaagaagaa tgcattcaga agaagattat agtaggatgt gggtatatct ttatgaagat
    16501 atagttagat ttaatgatat atctatagca gttgaatatc catgtaaagt aaatgataga
    16561 tatttaatga atccatcacc tatacctaga tttgatataa aaaaattaaa tatgtcagac
    16621 aatttattct tgtttggtgc aggtagagaa aaaagaattt atgctattcc tccatataca
    16681 aaggtagaac ctttagagtt tgaggattat aagtttgaag aggaaaaatt tgaaggaaaa
    16741 tactgtagtt tatgtaacag tacaaataca tttttagatg aagtatatga cagtgatact
    16801 aatgaaaagt actatagttg ttcagataca agttattgtg aaaaagttag attaaaaaat
    16861 aacagtatag atgtgacaat aggaggtact tggaatgagt aaaataaatg actctaaaag
    16921 attagaaagt atagacaagc aagtattaat aggaagaaat atcaatctta tttatggaga
    16981 tgggtgtgaa aagtgttttg aaagcacagg gtatgaaagc aataatatat gtaagtattg
    17041 tggttctata gttgcatgta ataatgtaaa tttagaactt tatgaaggag aagtccttgg
    17101 aattgtaggt gaatctggtt caggaaaaag cactttatta aaaatactgt tttttcaaga
    17161 acatgcaaat agtggagaag tatatatatc ccattacaat aaagacacaa atatactaaa
    17221 gctttcagac ccaaaaaaga gatacataaa gaatcatttt atgggtattg tgtatcaaaa
    17281 tccccacctt ggtttaaatt tagactttag tagtggtggt aatatagcag aaaaattatt
    17341 gatggcaaat ttatataatg ttgaaaaaat aagagataga ggcaaagaac ttttaataaa
    17401 aactaatata cctgtagaaa gaatagacca taaaccaaaa tactttagtg ggggaatgca
    17461 acaacgagtt caaatagcaa aagctctttc aaataaccct ccaatattgc tattagatga
    17521 ggttacaaca ggtttagatg tttctgtgca agcggaagta ctggatttaa taagagaaat
    17581 ccaaagagaa cttaagattt ctatgatagt tgtatcccat gactttgacg ttataaagat
    17641 gcttgctgat aggacaattg taatgaaaaa tggaagtgtg attgagtctg gtcttacaga
    17701 ccaaataatg gaggacccac aacacccata tactcagcaa cttataaatt cattgctata
    17761 aatatcgttt gtaaataagc aaaatggggg tttttaataa aatgaaagaa actatacttg
    17821 aagtaaataa ttttagtaaa gactttgagt tacatgcatt gaacaagact ataacggcat
    17881 gtagtaatat aagttttgaa gtttcaaaag gtgagttttt aggaataata ggtaaatctg
    17941 gagcaggtaa gtctaccata ttaaaaagta tctataaaac atatatacca acaactggag
    18001 aaataatttt taattcagaa atatatggta atgtagacct ggctaaaatt ggagaccgag
    18061 aaatgataaa tcttagaaag aaagaaatag gatatgtatc acagttttta aagacacttc
    18121 cacgaataac tgcaattgaa ctagtagtac attctttaat tgagagtggg tttgaaaaag
    18181 attgttcgta tgatatggca aaagatatac ttaatcaatt tgaaataaag gaaaatttat
    18241 gggatgctta tccaaataat ttttctggtg gagaaaaatt aagattaaat ttagctcaag
    18301 ctatggttaa aaagcctaga cttttattac ttgatgaacc aactgcatca cttgataatc
    18361 aatctaaaat atatgtaaaa gaaaaaatgt tggaacttaa aagtcaagga actacaatga
    18421 taggaatttt tcatgatata gagtttatgg aaactgttat agataagacc tttactatgg
    18481 caaaagggac tataagtgaa agtggggtag cctagatgat aaaactaatt cataatgcaa
    18541 atattgtatt agaagatatg atattagaaa atggccattt aatcatagag ggagatacaa
    18601 taaaaaaagt atcaaatgat aagattgatt acgcatttga ctttaatgag atagatgaaa
    18661 taatagatgc aaaaaactta tatataatac ctggtattat agatattcac agtgatgcca
    18721 ttgaaaagga aatagagcca agaccaagta cattattacc atttaatatg gcattttatg
    18781 agctagataa aaaactacct gcaaacggaa taactacagt ttatcattct atatccttag
    18841 gggatggagt aggagttaga agtattgata actctttaaa gatgattaaa aatattgatt
    18901 catataaaaa tatag
//
LOCUS       DAEMAE010000058        18785 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEMAE010000058 DAEMAE010000000
VERSION     DAEMAE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.059"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..1423
                     /locus_tag="KPH93_001614"
     CDS             206..1423
                     /locus_tag="KPH93_001614"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG1688441.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGADVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1518..1898
                     /locus_tag="KPH93_001615"
     CDS             1518..1898
                     /locus_tag="KPH93_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG1688442.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2181..2663
                     /locus_tag="KPH93_001616"
     CDS             2181..2663
                     /locus_tag="KPH93_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1688443.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIMVVLNHSEFSEINCMVENEVVAGKDTVIEIMK
                     "
     gene            2684..3499
                     /locus_tag="KPH93_001617"
     CDS             2684..3499
                     /locus_tag="KPH93_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1688444.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3552..5102
                     /locus_tag="KPH93_001618"
     CDS             3552..5102
                     /locus_tag="KPH93_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1688445.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFLKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQEIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATVIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5201..6553
                     /locus_tag="KPH93_001619"
     CDS             5201..6553
                     /locus_tag="KPH93_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1688446.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELDEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPASIVSEACRKLRP
                     NARIINICDMPIAIIDMVAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFDGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEISAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6684..7442
                     /locus_tag="KPH93_001620"
     CDS             6684..7442
                     /locus_tag="KPH93_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688447.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7489..8208
                     /locus_tag="KPH93_001621"
     CDS             7489..8208
                     /locus_tag="KPH93_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG1688448.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKKWLELDDFHKIKSIYEFV
                     QNDILFGYNTSDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHDGTFKKY
                     AIATNDLKNTSIDWNGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8332..9732)
                     /locus_tag="KPH93_001622"
     CDS             complement(8332..9732)
                     /locus_tag="KPH93_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG1688449.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLVARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLKNVGETQNLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGFLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRYQIIRS"
     gene            complement(9736..10644)
                     /locus_tag="KPH93_001623"
     CDS             complement(9736..10644)
                     /locus_tag="KPH93_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1688450.1"
                     /translation="MKGDNKVTEKQMLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAVKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10959..11606
                     /locus_tag="KPH93_001624"
     CDS             10959..11606
                     /locus_tag="KPH93_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1688451.1"
                     /translation="MKKKICVAIVFALFIVFAKMFIDSSSEERNVMKNELSKYRSGVN
                     VLSVNNIIVANKKYSLPENYSPQESIEARDAFFKMNEDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYIKEHGEEEANRFSAKPGESEHQTGLAFDIGGDDKSCWANQKFNNTKEAKWL
                     YENAYKYGFILRYPEGKEDITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11931..14570
                     /locus_tag="KPH93_001625"
     CDS             11931..14570
                     /locus_tag="KPH93_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG1688452.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILAILIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSILFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQFSIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14628..15731)
                     /locus_tag="KPH93_001626"
     CDS             complement(14628..15731)
                     /locus_tag="KPH93_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG1688453.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFITLDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPKVIKAFQIIDNIE"
     gene            16092..16913
                     /locus_tag="KPH93_001627"
     CDS             16092..16913
                     /locus_tag="KPH93_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1688454.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEEFIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16992..17203
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17290..18606
                     /gene="argH"
                     /locus_tag="KPH93_001628"
     CDS             17290..18606
                     /gene="argH"
                     /locus_tag="KPH93_001628"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG1688455.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLALDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKSTIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7191 a         2142 c         3301 g         6151 t
ORIGIN      
        1 taaaaaatat ttttaaatta tcatttaatg taatttaaat gaattgaaga tatatgttta
       61 ttgtatgcaa ttgttgtatt tttaggtttt ttgtgattaa ttaaaaaatt ataaaaatcg
      121 tttacatgtt gatttttaaa aaaaaaagat atagaattat atagaaagta ataaacaaaa
      181 attggattta cgggagtgga atgaaatgat gaatgttacc ttaacagatg tgaaagaggc
      241 tagagagacc attaagaaca tagtaaaaag aactgactta cttgaaagtg ttaaacttag
      301 cgaaaaaaca ggagcaaatg tgttctataa atgtgagaat cttcaaaaaa caggttcttt
      361 taaattaaga ggagcttgta acaaaatagc gagtttgaca gatgaagaaa aagcaaatgg
      421 agttatagct tcaagtgctg ggaatcatgc tcaaggagtt gcattggggg caaaaatgac
      481 tggtataaaa tctacaatag taatgccagc aactgcacct ttagcaaaag ttagtgcaac
      541 taaaggttat ggagcagatg tagttttaaa tggtgcggtt tatgatgatg catatgcaaa
      601 agctgttgaa atacaaaaag aaacaggagc aactttctta catccattca atgataagta
      661 tgttatagca ggacaaggaa ctatatctct tgaaattttt gaacaattag acaataaagt
      721 tgatactata ctatgcccag ttggtggagg aggaatcatc tctggtgttg ctgttgcagc
      781 aaaagcatta aatcctaatg tgaaaatagt tggtgttcaa actgcaaata taccttcaat
      841 gaaagagtca ataaaaaatg gaaaagtaac aacagcattt aatgatacta caatagctga
      901 tggtatagct gttaaaactc caggagattt aacttttgaa ataataaatg aattagttga
      961 tgaaatagta gtggttgaag agacagaaat tgcagaatca atattattta tgatggaaag
     1021 tcaaaaaata gtttcagaag gtgctggtgc tgtatgtaca gctgctatat taagtggtaa
     1081 gtatgtacca gcaaaagatg aaaatgtagt ttgtataata tctggtggta atatagacat
     1141 aaatacatta tatagaataa taggtgttgc tttagctaaa gaaggaagaa gatattcatt
     1201 tagtacaatt atggaagata aaccaggaaa ctttgctgaa ttaactagga taataagtga
     1261 gaatggtgga aacattttaa gtgctaatca aggtaaatta tcagcagggg aagctctagg
     1321 aaaacaaagt gcggaattta tattagagac aatagattat gaccatatag ctagaataaa
     1381 gaaagctata gaagaaaaag gttttaaaat tatagaatta taataaaaag tgtatataat
     1441 atacaagaaa aatagtataa tagtgtatga agatacattt tatgtgtatt agattaacga
     1501 attaggagga ttttaaaatg aaacatgaag taatacatac taatgatgca ccagcagctt
     1561 taggaccata ttctcaagct ataaaagctg gaaacttatt atttgtatct ggacaagttc
     1621 ctcttgtacc agagactatg gaagtagttg aaggagatgt acaagctcaa actgctcaat
     1681 ctttaaaaaa tcttaaagct atattagctg aaagtggagc tgatttttca aatgtagtta
     1741 aaactacagt atttataaaa gatatgaatg aatttggggc tataaacgaa gtttatgctg
     1801 aatattttgg agaaaataaa ccagcaagag cttgtgtaga agttgctaga ttaccaaaag
     1861 acgttaaagt tgaaatagaa gtaatagctg tattataaaa ataaatactt ttaatttgaa
     1921 aaggctgaag aactatattc tagtgattca gcctttttat aaaaaaataa atcacaataa
     1981 agagcatgat aaatattgtc gaattaaaaa ataaaattaa aataaatatt gcaatatttt
     2041 tgagaaggtt ttataatgag tatataattt gaaacgtgta actattaaat taggcatgag
     2101 ttcttggata tttttatcct gaggattcat gccttttttg tattgaaagt caaaaattta
     2161 tttaataagg aggaagtact gtgttaaatt ttttcaagaa aaacaaaagc tataagttac
     2221 atgcagtagt gagtggtaac agcattaata ttgagaaagt aaacgattct gtgttttcaa
     2281 aaaagttaat gggagatgga gttgcaatta ttcctaatag taatgttgtt gtagctcctt
     2341 gtaatggtaa agtaactgtg cttacagagt caaagcatgc atttggtatg gtctcagatg
     2401 agggtgttga aatattagtc cacataggaa ttgatacagt taatttacag ggtgaggggt
     2461 ttaaaaatga agtgtctcaa ggagatactg ttaaaaaagg tagtccaata ataagctttg
     2521 aaagagaaaa aataaattct cagggaattg attgcacaac aataatggtt gtattaaatc
     2581 atagcgaatt ttcagaaata aattgcatgg tagaaaatga agttgttgca gggaaagata
     2641 ctgttattga aattatgaaa taataggaag ggattaattc aatatgagag tagtaaaaat
     2701 ctttaataat aatgcattat ctacagtaac taatgatagg aaagaagcta ttttactagg
     2761 tcttggtata ggttttaata aacgtccagg agataaagtt aatgaagata aaatagaaaa
     2821 gatttattat gttcaagacc acatgcaaac aaaattttta gaattattaa aaaatgttag
     2881 tccagaagtg atggatgctt cacaacaaat tataagtctt ataccaggaa atgaggggaa
     2941 atttaataat aaagggatat tatctttagt ggaacatatt agttttgcaa tagatagaat
     3001 gaaaaataat gtttttttac ctaatttaat gcttttggat ataaagatga tgtattcaaa
     3061 agagtttgaa ttaggagtaa aagctttaga aattatatac agagtatgtc atatccattt
     3121 gcctgaagat gaagcaggtt acatagcttt acattttgtc aatttacaat caaatgataa
     3181 tttggcatat gatactttaa aatttgtaaa aggtagtatt gatttgatta aagaatgtta
     3241 tggattagaa ttagacgaat cgagtttgag tacattacgt tttagaactc atcttaaatt
     3301 tcttgctcaa agaattttta agaatgaaat ttgtcaggat gataaaatga ttgaaatgta
     3361 tgattattta attaacaatc atcctaaaaa taaagaatat ttagaaaagt taaatgtata
     3421 tattgaaaag gagtttagat ataaattaga taaacctgaa aaaatttatc tactactaca
     3481 cctaacaaaa atcttataag aatgagatta attagtaata gaaatatgta taattttaag
     3541 gggggataag attgaaagag aaactacttg gaggactgga aacatttgca aaggcaatgg
     3601 ttcagccatt aatgtattta tcaagtgcag ggatattaat ggtggtaggt gtactactta
     3661 caaatgatat gttattagga gtacttccat ttttaaaatg ggaaccaatt caaatatttg
     3721 gaagcttaat ttataattgt ataatggcta ttattaataa tcttgctgtt atatttgcta
     3781 taggtattcc tgctgcacta gcaaaaaaag agaagcacaa agcagcgtta ataggattta
     3841 tgagttatat aatatacctt acagcttcta acactatgtt aacatcacta ggtcaactag
     3901 caaaagaaag caagatgctt ggactgatag ggactggaca agctactatt ttaggtattc
     3961 aagttgtaga cacaagtgta tttggtggaa ttattttagg gtgtatagct ggatatgtat
     4021 ttaatcgtac aagcaacaaa acatttaaag gagctttaca gatatactcg ggagcaaatt
     4081 ttagttttat atgcatgttc tttgtatcca ttttaattgg agttgtgagt aactttatat
     4141 ggccattagc tcaacaagaa atagcttctc ttgcaaatgt catcaaagat acaggtacat
     4201 ttggtttatt cttatatggt tttttagaga gaatattagt accaactgga cttcatcatt
     4261 taatctatac accattccag ttttctgatt taggaggagt gcttaaagtt ggtgcagata
     4321 cagtttcagg agcctatgct atagttatga cagaaatgaa tttaccagtt gagcgtttct
     4381 cagatagtat atactacatg gcaactggat ttacaaagat gtttggttat ataggtatag
     4441 gagctgcctt tatttatact gcatataagc aaaataagaa gaaggttaag gcaacggtta
     4501 ttccgctaat aattacagca gtattagcct ctgtaacgga accattagac tttatgtttg
     4561 tattctgtgc accactttta ttctttgtac atgcagttat agctgggctt tttatagctt
     4621 tattaaaaat atttgatgtt actgcactat gtggaggtaa cttattagta tcaattataa
     4681 tgaatactac agtaggtgta gaaaaaacac actggccagt aatgatgata ttaggacttg
     4741 cacaaattat tgtatatttt gtagtattta gttttttaat taagaaattt aattataaaa
     4801 ctccaggtcg tgaagatgag agtacagcta caggcgaaga acaatcaaaa caacttaatt
     4861 tagatgcagg tatagaaaat ataattgatg ggttaggcgg aaaagaaaat attaatacag
     4921 tagaaaattg catcactaga ttacgtgtta atgtaaaaga tgaaagtaag attaatgaag
     4981 atataattaa tttaacgcca aatagcggga ttgtaaggaa aggaaaagat atacaaataa
     5041 tttttggact tcatgtgcat gaggtaagac gtgcagtaga agatttttta gaagcttatt
     5101 aaagatatat atatttatat agcaagggta aataacagct ttaagtgctg tttactattt
     5161 attaaagata catataattt atagaggagg aataattatt atgattatta ctattgttgg
     5221 tggaggaagc acatttactc caggtattgt taagtcaatt gcattaagag aaaaagagtt
     5281 agagcttgat gaaattcgct tatttgatat tgataaagaa cgtcaagata aagtagctgt
     5341 agttgtcaac tggatactaa aagaagatgt taaaacagat gttaaactaa ctgtaaccaa
     5401 ttgtgaaaaa gaagcttata cagatgctac atttatattt gcacaaatgc gtgttggtaa
     5461 atatgaaatg cgtgagcagg atgaaaaaat tccattgaaa tatggttgtg taggtcaaga
     5521 aacttgtggc tgtgggggga tggcatatgg attgcgtaca atacaaccaa tgataaaggt
     5581 tattgatgat gtagagaaat atgctaaaaa agattattgg atattaaatt actctaatcc
     5641 tgcttcaatt gtttctgaag cttgtcgtaa gttaagacca aatgcaagga ttataaatat
     5701 ctgtgatatg ccaatagcta ttatagatat ggtagcgggg tcattaaata tcaatgacgt
     5761 tcataatata cgctatgatt attttggtct aaatcatttt ggctggttta cttcaataga
     5821 ttataaacat agagacttaa tggaagagat taaggaatac ataaaagaaa ataaaatatt
     5881 actgccaaaa tcttatatag aaaacttaaa taaaagtcgt agtacaggaa atcgccatgc
     5941 tgtaagtagc tggatacatg tttgggaatc tgtttataca atgttagaaa gcttcccaaa
     6001 atatctgcca aacacttatt taacatacta tcttttaagt aaagaaacag tggaacattc
     6061 aaacataaat cgtactcgtg caaatgaggt tatggaaagc cgtgaaaaag acttatttga
     6121 tggtgtgaga cattacttag aaactggaga aatttctgaa aaagcatttt atgcaggttc
     6181 acatggagat tggattagtg atttggcagt ttcaattaaa aatgatacac gtagtcgttt
     6241 tttagttata actgaaaatc gtggagctat accaaacatg ccatatgatg caatggtaga
     6301 aatttcagca tatattggta aagatggtcc agaagttatt gctcgaggtc caatacctct
     6361 attccaacaa ggattaatga tgcaacaact taacagtgaa aaattgatag tagaagctta
     6421 tatagagaat agttatgaaa aagctttaca agcattcaca ttaaataaaa ctattccaag
     6481 tatgaatgtt gctaaagaaa ttttggatga aatgatagaa gttaataagg aatactggcc
     6541 agaattaaga tagtaggata aaagaaaggg tttttattta ataattagat atgttttcag
     6601 agtcttttat caaaatgtat tcacaaacat atggcaatat attaaaataa agagtgatat
     6661 ttttagaaga atggggtgat gcaatgaaca ttataaataa aattttttgg caagaagata
     6721 ggattggcat gattacaaat aatttagagg cagatacaca tagccattgc atgttacaac
     6781 tgttcttagg gatagaagac agcattgaaa taacagtcaa tgaaaaatta gttaaatgta
     6841 attgtatcat agtagataaa aatatctctc attcattttc agcaagaaaa aaagtttatt
     6901 actctgctat tattgaacct acatctatat atgcagaaca gcttacctca aaaatgaatg
     6961 attttggcta ctggatatgt gacaatgatg gattagagaa attacgtcaa caaggaactt
     7021 ttttgatata taactctagt aaagaacaat atttaagatt tatggaaatg cttaataact
     7081 atttgaatat acctataact ctcaaacatt atgatgacag aattactgaa ttgctcaatt
     7141 tattacgtac ttgtaattgt gataatcata ctatttcaag ttttgcagat aaagtgtccc
     7201 tatcagcaag taggttatca catctgttta aagaacaaat agggatacct ttaaaaagtt
     7261 atatcttatt tcatcaaatg gagtgtgctt ttagagaatt actttctgga aaaaatgtta
     7321 cggaagcttc tatgactgct ggattcgata ctccatcaca ctttgcaggg acagttaagc
     7381 gaatgatggg aatgcccgtt tctttatctt taaaagatag cgagtttttg aaagtatatt
     7441 aaatattctc gttatataat gaagttgtac taagaaaggg gttttattat gaataaagaa
     7501 tatttagaag aaacaaaaat gttaaattat aatgaaccac aacttaagct tttggtatca
     7561 tcaaagaaat ggttggaatt agatgatttt cataaaataa aatctattta tgagtttgta
     7621 cagaatgata ttttatttgg atataatact tctgatatgt taagcgcaac acaggtatta
     7681 aatgatggta tgggacaatg caatacaaaa gctactcttt taatggcatt attacgcgca
     7741 gtgaatatcc cttgtaggct tcatgctttt gatgtaacaa aagattttca acgaggtgct
     7801 acatccaaat tgatttcact attagcacca aaatatattc ttcatacttg ggtagaggta
     7861 ttttatcaag ataggtggat tgctctggag ggtgtcataa ctgataaaaa atatttagaa
     7921 gcaattcaga agaaattttt taatcatgat ggaacgttta aaaagtatgc aatagctaca
     7981 aatgatttga aaaatacatc tattgattgg aatggtaaag atacttttat ccaaaaagaa
     8041 gctattgtgt atgattatgg tatttttcca tcaccagatg tatttttttc tactcattca
     8101 caacatatgt caaaattaaa aaatttcata tatgtacatc ttatcagaaa aataatgaca
     8161 aaaaatgtat gtaaggctag aaacaattat attgataaaa acgaatgagt gtttaagagg
     8221 tgactatata ggaaatatat agaataatta aaactgtact ggttaaaatg aataaaaagg
     8281 agttatcaca gagcaaactt aaattgttca taggataact ccttttttgt attaacttct
     8341 aataatttga tatcttttta taaaacatat agatacaatt aaaccagtaa atgcaagagt
     8401 agctgctatt gaaaatgcaa catctattcc atgaatgtta gacaacatgt ttgaactcat
     8461 atgagctttc ttagtagcac ttgtcattat agtaataagt atagcagaac ctatagctcc
     8521 agatatctga cggagtgtgt tattaattgc tgtggcatga gatataagtt ctctatctaa
     8581 tgttttaatt ccccatgtag ttaatggcat taaaaccatt gaaataccaa tcatacgaat
     8641 acaatacatc aatgacacat gaattaaaga tgtatccctg cctaaaaaag cgaaggataa
     8701 agttcctaaa aacaaaaatc ctgttcctaa tattgataga gcatgaggtc catatttatc
     8761 taaatgacgt cctgcaattg gattgagtac aaccatcaat atagcaccag gtaacattaa
     8821 tgaacctgaa gataatgcag aaaatcctct tacagattga acgtatagag gaatcattaa
     8881 tgtagcagac atcattgagg catatacaat cataattaat attgtagaaa taagaaatgg
     8941 tttattttta aaaactctca attctaaaaa aggttcttta gattttaatt gacgcaatgt
     9001 aaataaaatt aatgacataa ttcctattaa tattggtaaa tatgtggcaa tattagtcca
     9061 cccataatta ccttgattag taaatccaat taataaacca ccaaaaccta gagatgatag
     9121 tactattgaa ggtatatcta attttaagtt ttgagtttcc ccgacatttt ttaatagaat
     9181 aaatgatagt ataacatcta atatagcaat aggggaaaga aacagaaata agctatgcca
     9241 accaaatgaa tctactaacc atcctgataa agttggacct attgctggag caaatcctac
     9301 cgtaatacca actaaactca tagctgcacc acgtttttcc tcagggcata aatagagtat
     9361 aataacttgt aaaagtggca ttagagaacc agaacccatt gcctgcaata cacgagcaac
     9421 caacatagtt gaaaaattat tagaaaaaag tgcaataata catcctatag aaaagataga
     9481 cattgatgta ataaataact ttctagtact aaatctattt attaaataag ctgttgtggg
     9541 tacaataata cctgcaataa gtagataaat tgttgttaac cactgaccaa catcagcagt
     9601 aattttaaaa tctgacatta tattaggtaa tgctgttgac aatagtgttt gactaagaga
     9661 acttataaaa gcaccagtca ttacaattgc tattattaaa tttccttttt tattgttact
     9721 ttgggtttcc ataatttata acgtctcctt ttttaattga tttttaatac tgttcaataa
     9781 aagtactttt ggtcttaaag tgtcttcttt ttctaaatat gcaatcattg catccataat
     9841 taaagaatca tattgttgat tttttccctc gacgtataat ttttttactg cttctagtaa
     9901 tttaggattt tcaaggagag aagtatccgt tttgattagc tcttctatat cttgtatcgt
     9961 tttaaaaaat agcgatttat tatgaaggga aaagtaaact tcatcttctt gtttatgtaa
    10021 aaacattgat ttttcaatta aaggatttag attatccata ttttctacaa ctaaatctag
    10081 aatttgtata gtaaatttgg caataaagta tacatttatt tctctttttt gtacatgaat
    10141 aatgtatata aattgatgaa ttaaagcatc taataagaaa actaaatcaa atatgatttt
    10201 ttctatttta tcaccatagt tatcaaataa gcttaatttg tattcattca ataagttagc
    10261 tcgtgatttt gtttgcaatt tacttgtagc cttacgttgc tcttcagaaa agttttctct
    10321 tatgtaggta tttatagtag ttctagaaaa aatatttttc catacagcaa ttatctttct
    10381 gtttaacttt tctgatgatg gaatgtcatt attttcattt atggaatgta taatttctaa
    10441 aaacttttca tcataataat tgattatttc gcagataaaa ctttccttag tttcaaaatg
    10501 cttgtaaaaa gtagctttag acattttaca ctcttttaca acatccgata ttgaagtatt
    10561 ttgaaaacct ttttggtcaa aaattttttg tgcaatagtt ataatgagca tttgtttttc
    10621 tgtcacttta ttatctcctt tcatatgaaa acattaagta ctatatagta cttataagtt
    10681 ttcatagata tattatacat cattttgaaa aaaaatcaat atatgtacta taatatgaaa
    10741 tttttgaaaa atttattgtt atttttactc gtataaaaaa aattaagagt ttcttaagaa
    10801 ttgtaagaat ttcttaagaa ttgtaagagt ttcttaagat ttgtttaaag atgttgcaat
    10861 aagttagaag ttgcaataaa ttagtggtat gattatagca caagaaaaaa tgttttgaaa
    10921 attttctgta ttaggttaaa attacggagg tgtagtatat gaaaaagaaa atttgtgtag
    10981 caatagtttt tgctctattt atagtttttg caaaaatgtt tattgacagt tctagtgaag
    11041 aaagaaacgt tatgaaaaat gaattaagta aatataggtc aggtgtaaat gtattatcag
    11101 taaacaatat tatagtagcg aataaaaaat acagtttacc tgaaaactat tctccacagg
    11161 aaagtattga agcaagagat gcttttttta aaatgaatga agatgctcaa aaatcaggat
    11221 taaatttaaa agcttttagt acatatagaa gttatgaata tcaagataga ttatttaaat
    11281 catacataaa agaacatggg gaagaagaag caaatagatt ttcagctaaa cctggagaaa
    11341 gtgaacatca aactggactt gcctttgata ttggaggaga tgacaagtct tgttgggcaa
    11401 atcaaaagtt caacaataca aaagaagcta agtggttgta tgaaaatgca tataaatatg
    11461 gttttatact tagatatcca gaaggtaaag aagatataac aggatatatg tatgaatctt
    11521 ggcactatag atatgttgga actgaacata gcaaaaactt tgcaatgaat aatttgactt
    11581 tagaagaata tttacatata aattaagaaa tggtttgtta aaattaggtt aaaataaaaa
    11641 ataacctaga ctattaattg tgtaaagtga tataatgtaa atacaattta agataactac
    11701 tcgaagggga gtagcttaca cgaaagtggt aattaatcgt caagacagta gaaatactcg
    11761 gttaattagc aagggattcc ttgatagcaa gaccttcaaa ataagcatta ggtatgtttg
    11821 ttttggggtc tttttttatt caaggaattt ttatttaaaa aaatttaaat ttcaagtata
    11881 cataagagcc cccaaacata aaataaattt attaaagagg agggcaacaa atgtcttaca
    11941 aaagtactta tcaagaagta attaaaaatt tagactcaag cttatctggt ttgagtaaag
    12001 aaagagcaga agagctgtta gaaaaaaatg gagctaatga attaaaagaa gcagataagg
    12061 ttcctactta taagcttttc ttagaaagtt ttaaagaccc attagtaata attctattga
    12121 tagctgcttt agtacaaata tttttaggtg aaaccgtgga gtctataata atttttgcag
    12181 ttattattat aaactctgtt ctaggtgttg tacaaacaaa aaaagcagaa agttcattag
    12241 aaagtttgaa aaatttatct gcaccaaacg caaaggtaat aagagacaat aaaaaaatga
    12301 caattccagc aaaaaattta gtagttggag atattgtatt cttagaagct ggcgattaca
    12361 taccagcaga tggaagactt atagaagcac aatccctaaa ggttgtagaa ggtatgttaa
    12421 ctggagaatc agaaccagtt ttaaagcata cagaaaaaat aagtgaagat gtaagtttag
    12481 gtgaccagaa aaatatggta tttagtggcg ctgtagttgt ttatggtaga ggttcttttg
    12541 tagtgactgg aacaggtatg gatactgaaa tgggtaaaat agctgggctc ttagaaagtg
    12601 ctgaaaataa acttacacca ttgcaacaaa aaatagagga ttttagtaaa aaacttggtg
    12661 taggtattgt aatacttgct attttaatat ttataattca agtggcaaga aattacttta
    12721 tggctggaaa tggagaaatg acgcaaatta ttttagattc attcatgttc tctgtagcag
    12781 tagcagtggc agctatacca gaagctctat catcaatagt cactatagtg ttggctgttg
    12841 gaacaaatag tatggcaaaa aaacaagcta taataagaaa attaccagca gtagaaactt
    12901 taggttcaac aagtgtaata tgtactgata aaactggtac tcttacacaa aataaaatga
    12961 cagtagttga ttattatatg tatggaacta ataatgatga gttagataaa gaaaaagtaa
    13021 attattctta tcatgctaaa ttattgacta tggtttctac attgtgtaat gactctcata
    13081 taactagtga tggtaaagaa gttggtgacc caacagaagt agctttaata aattattcaa
    13141 gtaaaaatga tttagattat gataaactaa gaagtaagta tatcagaata aatgaaatac
    13201 catttgactc agatagaaag cttatgtcta ctgtaaatag tatctatgga gattatataa
    13261 tgtttacaaa aggtgctcca gatattgtgt ttagtagatg taaatatgct ttgaaaaatg
    13321 gttcaaaagt agatataaca gatgagataa tagaagaata taaacataaa aatgaagagt
    13381 tttcaaatag agcacttaga gtattagctt ttgcaataaa agatgttcca gaagaagatt
    13441 ttataccttc aattgatgat gaacatgaca tgacattggt tggaataatg gcaatgatag
    13501 accccccaag agaagaagta atggatgctg taaaagaggc aaagagtgct ggtataaaaa
    13561 ctgttatgat aactggtgac cataaaacaa cagcagcagc aattgcaaaa gagattggta
    13621 taatggaaga aggggactta gctcttactg gaaaagagct tgatgctttg agtgaagaac
    13681 aactatatga aaaactagaa aatataagtg tatatgccag agtctcacca gaaaataaaa
    13741 ttcgtatagt taaggcatgg caacataaga ataatataac agcaatgact ggtgatggtg
    13801 ttaatgatgc acctgccttg aaacaagcag atataggaat aggtatggga agtggtactg
    13861 atgtagcaaa agacgcttct gctatggtgt tagttgatga taactttgca agtatagtaa
    13921 acgctgttga agttggtaga acagtataca gtaatataat aaaatcaatt acttatctat
    13981 ttgcaggaaa cttaggagct atagtatcca tattatttgc ggtatttgca ggttggtcaa
    14041 atccatttac agctttacaa cttttattta taaacttagt aaatgactct ttaccagcaa
    14101 ttgcattagg gtttgaaaag tcagaaaaaa atataatgaa taaacctcct agacatccag
    14161 atgaaagtat actagcagga ggaacattga aggcagttgc tataagaggt tgtataatag
    14221 gtgtagttac aataattgca caatatatag gattacaagt atctccagaa cttggagtag
    14281 caatggcatt ttcaacattg atattagcta gaataatgca aacattacct gcacgttcta
    14341 ataatcaaac agtaataaaa ttaggttttt tcagtaataa atacgttata ggagcagttt
    14401 tagtctgtct tgggctttac tctttaacac taataccagt gctaagagga atattctcta
    14461 taccaacaac atttggattg taccagttta gcatttgctt aggtttagca ttaatttcat
    14521 caatattaat ggaacttaac aaattaataa atagaagtga agtaaaataa tcaaaaaatt
    14581 aaagggcata ttgattttaa caatatgcct tttataaata gtctaattta ttctatatta
    14641 tcgattattt ggaatgcttt tataacttta ggtaattctt tttcttcagg cattctaaat
    14701 cttatgaaat ttttatcaag tccagtgaaa tcacttccag atataactct tatttttaat
    14761 tttgcaaatt cttgttgtaa atctatatcc ttgttcttat gttcaactgt cattattgat
    14821 acagtatcac tagtttcagc tatattaagg tttttccatg gaattaataa ttgatttttt
    14881 atatcttttg ttttttcttt taattcttct ataaattgaa catcatctaa taaatttgct
    14941 gcaatactac gagatatttc agacacttcg taaggagtag aaatcttttt aatagggcta
    15001 acaagttgtt ctggaagaac tgcatatcct gctcttagtc ctgctaagcc aaatcctttt
    15061 gaaaatgttt ttaaagcaat tacattatca taattattta gaagcttgac agctgaattt
    15121 tcttttggca tgtattcacc ataagcttca tctaccataa ctgctatatc atatttagct
    15181 gcttccttta ctatattttc aattgatgat aaaggtataa tttgtcctgt aggattgttt
    15241 ggattgtcta tgtaaattac tttatattct ggatttattt tttcaataaa ttctttttca
    15301 ttaaacttga aattatcctc tttctttaaa agaacataat cataagttgc accgtgcatt
    15361 tttatatctg tctcatattc tgagaactga ggtgaatatc ctaaaacttt atctcccttt
    15421 tcaataaata gtctatttaa aaggtaaata acatgtatag aaccatctgc caaacaaatt
    15481 ctgtcagtgt ctaatgttat aaagtctttc caataatcta ctatattatc ttttaaagca
    15541 attgaatgag gatatcctct tatcatatca aattttaaag cattaaaagc ttctacagct
    15601 ttgtttgagt aagacactgt atttatacct tcaccacagt ctatctcgaa tacatcttct
    15661 gaatgtccag attctattgc ataacttttt ttcaactgca tttcaatagc tttgttaatt
    15721 tttagtttca tactttatca ctccttattt agtcaataat aaatgttaaa aaagttgcta
    15781 gatttaaaat attatgaaaa tatataatag taaaaaatga atataaatat tataatagct
    15841 cactttattt aatatgatta tatatcatat attgtaaaat ttctagcatt ataagagtat
    15901 aaattgaaaa tattcagaaa aatactacaa gaaacgattt attttgctct taaaataagt
    15961 taaatgaatg ttattaattt tgtaaattga aatagtttaa gtataaagtt atgatataat
    16021 ctaataaggg taattcttta gattatatta aggaaatata ggtatacaat aaaaagttat
    16081 ggagttggat tatgggatat aaattaattg taacagatat ggatggaact ttattaggca
    16141 ataatcataa agttacagat gaaaataaaa ctgcactaca aaaagttata aaaagcggta
    16201 ttaatgtaac tttagcaaca ggaagagcct tcgattcagc taaatgcaat gtagattttc
    16261 ttaaagaaga catgccaata atagcttgta atggttcatt aataagagag caaaatggaa
    16321 atataatata ttcgaataag attgatacaa gaacatgttt aaacatttta gatgtattag
    16381 ataaatacga tatttactat caatgtaata gtatagattc tatgctttca aaaaaaatag
    16441 aaggacgtga agatagatta agtgtatttt taggctctga aacagaagta attgtaaaag
    16501 atgatttgag agaagaaatc tttaaaaaag atatactaaa atttgtaatc atagaagaaa
    16561 agaatccatc tatcttggat gaaataagaa aagaattgag aaaagttcaa ggaataaaga
    16621 taacctcttc atggccaaac aatattgaag tcatgaatga aggtgttgat aaaggtaatg
    16681 cagttaaaat tttagctgaa aaaatgaata tagatagaga agatataatt gcatttggag
    16741 acaattacaa tgacatagaa atgataaagt ttgctggtct tggggtagct atgggaaatg
    16801 cagaagaatt tattaaacaa gaagctgatt atgttacaga tacaaatcaa gacagtggag
    16861 ttgcaaaggc aatttataaa ttcctagagt taaaagaatc acttagttct taaaatagta
    16921 tataatagga aaaaataggt tgactatttt gtatatagta attataatac aaatagataa
    16981 aatataaaaa tttagattca gtgaaaaaga gagtagatat agagaaaatc tcagcgaatt
    17041 gggggatggt gtgagcctaa tgataacact ttatattgaa aagaactttg gagaatacta
    17101 cttgaaattt agtatggtag taggtaatct cgccttaaga gaatagagtg ggtaaacatt
    17161 gtttaccaac tagagtggta acacggaata tttcgtctct tggtcttttt gaccaagaga
    17221 ttttttattt tcattaagtt acaagaatta taattaaaat ataaaaaatt aaaatatgag
    17281 gtgaaagtta tgaaattatg gggcggacgc tttagaaaag cggaaaatca acttatggaa
    17341 gaatttaata aatcttttgg ctatgattgt gtactgtaca aaaaagatat cgaaggaagt
    17401 gtagcacatg ttcatatgca agtaaaatgt ggacttttaa cagaagaaga aggaaagtct
    17461 ataacagaag gtttaaaagg gatacttgaa gatgtagaaa atggaaagtt agcattggat
    17521 ggagagtatg aagatataca tagttttact gagataaatt taattcaaag aattggagat
    17581 gttggcaaga agcttcatac agcacgtagt agaaatgacc aagttgcagt tgatatgaga
    17641 ttgtatgcca aagaaaaagc aaatgatttg gtggatttaa tatctgaatt taaatctact
    17701 ataaaagatg ttgctgacaa aaacccggtg atgatgccag gttatactca tcttcaaaga
    17761 gcacaagtag tgacatttaa gcatcactta atggcatatt atagtatgtt tgatagagat
    17821 gagaaaagaa taaagaatgc tatagaaata ttagatgaat cacctttagg ctgtggggct
    17881 ttagcaggaa ctacacatga tatagacaga agtataactt gtgaacaatt aggatttaaa
    17941 aaagtagtag ataattttat ggatggagta agtgatagag attatttact agagctaatg
    18001 agtgattttt caattataat gatgcactta agtagattaa gtgaagagtt gatactttgg
    18061 agttctcaag aatttggatt tgttgagata gatgacttat atactactgg aagtagtata
    18121 atgcctcaga agaaaaatcc agatggtgca gagcttataa gaggtaaaac tggcagagta
    18181 tatggaaact tatttggtct ttttacagta atgaaaggaa taccattagc atataataaa
    18241 gacatgcaag aagataaaga aggttttttc gatagtgtac atactcttga aatgtgtata
    18301 caaataatgg atagaatgat agccacacta aaggtaaatg aggataaaat gaaacaggca
    18361 gtaaaaaatg gttttttaaa tgcaacagaa gttgcggatt atttggttaa aaataatgtt
    18421 gcttttagag atgcacatgg tatagtagga agtatcgtta tatattgtga agataataaa
    18481 aaagcaatag aagatttaac attagaagaa ttacataagt tttcagatgc ttttaaggaa
    18541 gatatttatg attttatcga ctatgagagt atattaaata agggaataaa aaagaattta
    18601 aaataatgta ttggatttct aaaagctgta agtttgaatg ttatcattta aatttacagc
    18661 tttttattgt gtaagaattt gatatatgaa agtaagaaac ttaaaagaag tagtatttaa
    18721 aataaatata aataaaaaat tattattaaa atgataatta atatcaataa gtattgacaa
    18781 aatta
//
LOCUS       DAEMAE010000059        18751 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEMAE010000059 DAEMAE010000000
VERSION     DAEMAE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.060"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..1044
                     /locus_tag="KPH93_001629"
     CDS             76..1044
                     /locus_tag="KPH93_001629"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG1688456.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILNMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYERNEFNKSCSVLREYIALSKTL"
     gene            complement(1161..2291)
                     /locus_tag="KPH93_001630"
     CDS             complement(1161..2291)
                     /locus_tag="KPH93_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688457.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNMENTLKLSNILNFPKEYFYRNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLEDLIHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEVDKKGWLIEEPLDKMKGSNPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEANKKSKVIKFDGKK"
     gene            complement(2444..3397)
                     /gene="msrA"
                     /locus_tag="KPH93_001631"
     CDS             complement(2444..3397)
                     /gene="msrA"
                     /locus_tag="KPH93_001631"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG1688458.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHLENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESKEYLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNDKEGIYVDI
                     VSGEVLFSSKDKFDAGCGWPSFSKPVIDKSIVERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPEELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3521..4426)
                     /locus_tag="KPH93_001632"
     CDS             complement(3521..4426)
                     /locus_tag="KPH93_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG1688459.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNHHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(4747..6339)
                     /gene="hcp"
                     /locus_tag="KPH93_001633"
                     /gene_synonym="priS"
     CDS             complement(4747..6339)
                     /gene="hcp"
                     /locus_tag="KPH93_001633"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG1688460.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTNETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEIEKSKLVPRCLDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYKEDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6399..7124)
                     /locus_tag="KPH93_001634"
     CDS             complement(6399..7124)
                     /locus_tag="KPH93_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1688461.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRG
                     VSNSASISNDIVETKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAKKLTEILKENDIKNIVVTRMEVPCCGGIVSAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7289..8005
                     /locus_tag="KPH93_001635"
     CDS             7289..8005
                     /locus_tag="KPH93_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1688462.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSNELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCDVLFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHKFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFCFPNKLNI"
     gene            complement(8130..8705)
                     /locus_tag="KPH93_001636"
     CDS             complement(8130..8705)
                     /locus_tag="KPH93_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1688463.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYKDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHTPRFGQKKLIMVYTQAAPVSFLFKKYFKYTSKHLKGMGLNHYKTIVAT
                     KAFERNSTKKNFKVLRKAYKLGMKIAIDING"
     gene            complement(8879..9301)
                     /locus_tag="KPH93_001637"
     CDS             complement(8879..9301)
                     /locus_tag="KPH93_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1688464.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNDIDNGELFRCVFTIEE
                     GESVEYFSLACIHCDEPKCLDICPKDCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(9285..11492)
                     /locus_tag="KPH93_001638"
     CDS             complement(9285..11492)
                     /locus_tag="KPH93_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1688465.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVKMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRYMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDDIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISILDAKELDVISGDYIKITTKTGSLDVKVNITDRVLY
                     RNIHMYHGYREADVNSIVCDNFDPYSGFPAYRSCKCKIEKMNRQMEGYNEKFI"
     gene            11773..14952
                     /locus_tag="KPH93_001639"
     CDS             11773..14952
                     /locus_tag="KPH93_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1688466.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGVDKDKLSNTNSKASLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNSHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQVLSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMV
                     DGNICCDVKYIYDDEDKKFVLPNTQKEAIIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFIELENFDVILTTYGTLRNDLEKYSEIKFDYCIIDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(15237..16001)
                     /locus_tag="KPH93_001640"
     CDS             complement(15237..16001)
                     /locus_tag="KPH93_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1688467.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSNMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEKMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISNDFFENTFSSLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(16239..17732)
                     /locus_tag="KPH93_001641"
     CDS             complement(16239..17732)
                     /locus_tag="KPH93_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1688468.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLEYNIPSRVQREVIPKLLKGQNLV
                     VKSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQPIKEQIAELKQRVHIVVATPGRILDHINKGTIKLENIQYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVGLFSATIDEEIKYICEKYMPDYSVINIEENEIDNNQRTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKDGFLIRELH
                     ADLSQERRIFVIKDFKKQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVNEKDEKYIENIETYIGYKINELTDIEQSRITQGKILFEEYSKE
                     ILKEVSKRKKADKNSYKSKDIKLNTESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(18060..18602)
                     /locus_tag="KPH93_001642"
     CDS             complement(18060..18602)
                     /locus_tag="KPH93_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1688469.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGK
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         6369 a         3047 c         2125 g         7210 t
ORIGIN      
        1 tatataaaaa cagcttttaa taacatctat aattttctta taaatgtatc gttattttag
       61 ggggagtgat ttaaaatggc aataaagcca agaaaaatat ctattgtagg ttcagggcat
      121 gttggttctc attgtggatt cagtttgata actcaaggag tttgtgatga actctttatg
      181 attgatatag atgaatctaa atcaaaagct caagctttag atttagctga tgcagtttca
      241 tatctgccac acaaagtaca tatagaaaaa ggaacttttt ctgactgtaa agattctgat
      301 atagtagtga taagtgttgc agactcttca gaagggcctt tacgtagaca aaacacaact
      361 agattagatt tgctaagacc aactattgga atgataaaat ctatagtaaa acccatagtt
      421 gactctggct ttgatggtat atttgtagtc atatctaatc ctgttgatgt agttacaaat
      481 tatatttggg aaaaatctgg ctttcctaaa aacaaagtca ttggaactgg cacagcttta
      541 gattcaacaa gacttagaag gattttatct gaagaaactg gaatcgctca acaatcaatt
      601 caagcttatt ctatgggtga acatggagat tctcaaatgg ttccttggtc tcatgtttca
      661 attggtggta aaccaatctt gaacatgata aaagacaacc caagtactta tagcaattta
      721 gacttaccat caatagttga aaaaaataaa aaaacaggga taagtataat aaatggtaaa
      781 gattgtacag aatttggtat aggtactgct ttagtagaaa ttgtaaaagc tatactacac
      841 aatgagaaaa aagttcttcc agtttctaca ttactagaag gtcaatataa cgaaagaaat
      901 gtatttgcag gtgttccatg tgttatagga aaagatggta ttgaagaaat tattgaaatc
      961 aatatgactg aatatgagcg aaacgaattt aataaatctt gttctgtttt aagagaatat
     1021 attgcacttt caaaaacact ttagattcaa gattctttct tacactaaaa gcaggtaaat
     1081 gacttattga aaagactttt aactaagtta atgtttaaat catttatacc tgcttttaat
     1141 tttaatacta aaatagcaac ctatttttta ccatcaaatt ttataacttt acttttttta
     1201 tttgcctctt gagataataa tccttttttc aatcccatca ataattctaa atcttctgga
     1261 taaagattta ttccaaattc ctctaaactt gatactatac tatttacact cattatttta
     1321 ttttctatca ataattcaac tgctcttttt aaatacgttg gatttgaacc tttcattttg
     1381 tctagtggtt cttctatcaa ccatcctttt ttatccactt cattcataag ataattgtat
     1441 tttttgtaat ttatcttttc tagattatat gctctaaaaa tcatagcaga tataggtaca
     1501 atccattttt tctttagttc tacgtaataa tccaaatctt ctggatgaat caaatcttct
     1561 aaaaaactct cttttggcaa taaaaatgca caagcatact catcttctga aaattgctta
     1621 cttggtattc ttaattcact tgatattata tatccaagct cacatgccaa agtataattt
     1681 ctaattgatg ctgacttttt gtcattaccc agagatatta gatatttaga ttctttacta
     1741 attctctgtt tttgagtaaa gatagttgcc ccttttctat ctacattcat acctgatatt
     1801 ataatccctt tagactctaa caaacttacc atatttacta ttggggtact atttaaatcc
     1861 caaaactctc tagtcttata tgcaagagat tctatatcat ttatattaat atctgacatt
     1921 acttttctat ttggcaagtt ttcttttgga aacttaatat aattctccat aaacgaatat
     1981 attttatgta ttataattat tttttctcta taagaaattt cttctactct aggtagttta
     2041 gattgagggt taaagtggct atcctctaca acgatattta tattttcatt tctataaaaa
     2101 tactcttttg gaaaatttaa tatattagat aattttaatg tgttttccat atttggtaca
     2161 tacttatttt cctcaaaagc ttttaaatcc tttttattta ttttagtttc tttagacaac
     2221 tgttctatcg tctttgctct aaaaattcta gctgatttta atctctttcc attaaagtct
     2281 tttaaattca tttgttttct ctctttcaaa attaaattct taatctattg gtataaataa
     2341 gattacaatt aataatttat catattattg acaaaaaaac aaaaggtgaa aatattttct
     2401 tatattttca ccttttagct aagtttataa taaatattcc taattactca aaaagtttta
     2461 aatattctcc atatcctaaa gcttccatat tttctcttgg tataaattta agagaagcag
     2521 aatttataca ataccttaaa cccccaagtt cttctggacc atcatcaaat acatgaccta
     2581 aatgagaatt tcctttagca cttcttacct caactctata cataccttgt gagaaatcac
     2641 ttctctcaac aatagatttg tctataactg gctttgagaa acttggccac ccacatccag
     2701 catcaaattt atcttttgaa gaaaataaaa cttctccact cacaatatca acataaattc
     2761 cttctttgtc attatcataa tattcatttt caaaaggcac ctctgtcata tcattttgag
     2821 tgacttcaaa ttgtatagga gttaaagtct tttttaactc ttccctgttt atattgtttc
     2881 catcccaatg actttttaca aaattatatc taccagaccc tttataatac atattgtagt
     2941 ttaatggtct ttttttataa taatcctgat gattttcttc tgcttcgtag aatttagttg
     3001 ctggaattat atcagtcact attttactat caaatatttt actattttcc agatattctt
     3061 ttgactctaa agctatattt ttttgctctt catcatgata aaatatagca gtcttatatt
     3121 gagttcccct atcaccaaat tgtccgccat catctgtagg attaatctgt ttccagaaga
     3181 tatttagaag ttcatcataa cttattatat catcatcata tgttatttga attgcttcta
     3241 aatgacccgt agttcctgaa catacctctt gatatgtagg gttttcaaga tgaccacctg
     3301 tatatccaga aactacttta ataactccat catacttatc aaaaggtttt accatgcacc
     3361 aaaaacatcc acctgcaaaa gtagcaagtt ttatcatttt catcactcct taaataaatt
     3421 atactttatt tattccctaa gaagtaactt aataatctgc attttattat ttttacctac
     3481 tcaatgaaat catgtgtaaa aatacaattt caaattgaat ctaatttcta taaaagctct
     3541 ttttctttat ccatccaaaa gcatctttta tatcacaatt ttctcctaaa gacattagat
     3601 attctaatgc atcacaatca tccatagcta catctttttc catcttagga tttaatctat
     3661 agtaattatc tccaagaagt ttttgagaaa ataactgact catttgagaa tttccttcta
     3721 atgtaataga tataattggt aaatcgggtt ctttattaat tatccagtca atagctcccc
     3781 attcagtagt atctgccttt atactattat atacatatcc agtccctatt gaaaaaagtc
     3841 ttgtattctt aagcttaaat ccactgtcaa tggcatgaat tatacacgct aaacttgggt
     3901 cagttgctat tattcctcca tcaacatggt gattatatgt tggaaaaaat actggagcag
     3961 cactacttgc cattgctaca tctacaactt tgctagtctt tgtaaaagaa tttggcatat
     4021 tgttataaaa cactggtttc catgcatttt cctcatttcc tatataaaaa cttggtatca
     4081 ttactaattt atttaaatct tttaattcta gttcctctgg aaatatactt aataaaactt
     4141 cttttaagtt tttgttttca tactttggtc tgagtatttc tgcataactt ttattaaaaa
     4201 tatactttga attttcaatt gagtaaagtt cttttatctc tttagggctt acaccatatg
     4261 caagtcctag tgcaattaga ctccctgtag atgtacctcc aaacatattt atattgttta
     4321 atagatttgg atactctttt actatcctct caagtatgga tatactcaat gcccctttta
     4381 atccaccacc atcaaatgcc aatatattaa aattagaatt tttcatatac atataccccc
     4441 acataaaaca tcttatattt taaattttaa tcaaaataat ttcactatag tatatatatt
     4501 ttatgcaagt tacatattac aatgttcctc ttttaatatt taagttgcat tcaaaccttt
     4561 aatacaaaga ttcttaaaaa gaatatactg aaagcaggtt tatttttcaa ttattacaaa
     4621 aaataaataa agggtcgtct caaaattgat tttttcaatc aaattttaag tttaacccct
     4681 tatcttaatt gtgtcatact taaaaatatt ttaactataa atatatttaa gtatagatat
     4741 atttatttat cctaaaatct tcttcatatc ttcttcagga gtagatatag gtgtcaatgc
     4801 aaatttatca accaatatat ctaatacatt cttagataag aatgctggca aagaaggccc
     4861 tatatagata tttttgatac ctaaatgaag tagtgtaaga agtatacaaa cagctttctg
     4921 ctcataccat gacagtacca tgctaagagg caaatcattt actccacatt caaaagcatt
     4981 tgccaatgaa actgccactt taattgcact ataagcatca ttacattgtc ccatatccat
     5041 taaacgaggt aagccaccta tatctccaag gtctaaatca ttaaatctat attttccaca
     5101 tgcaagtgtc aatataacag tgtctttagg agcttgtttt acaaattcag tgtaataatt
     5161 tcttccaggt tttgcaccat cacaacctcc aaccaataaa aagtgtctta tagctccact
     5221 ctttacagca tctactactt tatcttgtac agatgaaact gtacctcttg caaaaccagt
     5281 agttaatgta tctcctccat taataccagt catatgaaca tcttctttat atcctccaag
     5341 ttcaagagct ttttctatta cactagaaaa atctttatct tcatcaatat gaaccatacc
     5401 tggataagca actacttctg tagtaaatac tctatctcta taactatctt taacaggcat
     5461 taaacagttt gttgtaaata atattggagc aggaatatta tcaaattctt tttgttggtt
     5521 ttgccatgct gtaccaaaat taccttttag atgtttatat tcttttagtt ttggatatgc
     5581 atgtgctggt aataactcac catgagtata tatatttata cccttatctt ttgtttgttc
     5641 taataatttg tgtaaatcat aaaggtcatg tcctgtaact acaataaatg gtcctttctc
     5701 gattttcata gatacctgag tcggttctgg tgttccataa gtttcagtat ttgctctatc
     5761 aagcaattcc atacatttta agtttatttc cccagttttc ataactagac ctaaaagttc
     5821 gtcaatagac aagtctcctc caactgctat taatccttca tagaaaaact catttacgtc
     5881 tttgtctgta taaccaagta ccattgcatg atatgcataa gcagccatac ctcttaatcc
     5941 aaataagatt aatgatttta atgaacgtat atcctcatta tcattccata aatccttcat
     6001 atcatagtta tcatttctat tacaagttgt tgaacagtct aaacatctag gtacaagttt
     6061 acttttttct atctcagttt tttcaatttg tctttttata gcttcatcat caaaacttac
     6121 attcgttata gtagtgaata acccctctat cataatttta tctgtttcat ttgttggtct
     6181 attcttgttt ccaatagttg ctcttgaaag accaatcaac actcctgtaa gttcgtcttg
     6241 taagttggct gtacccccct ttttaccaca aacaccattt atagtacaac ctttacctcc
     6301 tgctgtttgt tcacattgaa aacaaaacat tttttccatt ttttattcct ccctttttat
     6361 ttattaaaat ttattatatt tttctataat tcacttaatt aaatttctcc atccacaatt
     6421 tttccatcta cagatatagt aactatttgc catggtatca tttttccact attttgtaga
     6481 gctgtcttaa cagcacttac tataccacca caacaaggaa cttccatacg tgttactact
     6541 atatttttaa tatcattttc ttttaaaatc tctgtaagtt ttttagcata gtcaacttca
     6601 tcaagtttag ggcatccgat taatgtaacc ttatttttca taaatttatt atgaaaattt
     6661 ccatatgcat acgccgtaca gtctgctgct attaaaagac ttgcattctt taaatatgat
     6721 gcattagttg gtactaattt tatttgtact ggccattgat taagttgaga accttttgtc
     6781 tctactatat cattgcttat agatgcacta tttgaaaccc ctctatttat tgacttagac
     6841 tgagaacctg gacatccaca tgctaaagtt ttcttttgtg ctttctttgc ttccatattt
     6901 gcttttactg cagcttcatc atattctgct gcttctctat actcaaatga aatcgcattt
     6961 gttggacaag ttggcagaca atctcccagc ccatcacaat agtcatctct taaaagtttt
     7021 gcttttccat ttaccattcc tatagcttct tcatgacaag cttcaacaca tagtccacaa
     7081 ccattacact ttccctcatc aattttaatt atttttctca tcatttatat cacctcataa
     7141 aattttttta atttaaagta ttgatttctt atatatttat ttttaatact tatatttttt
     7201 tatttcaaat ttcttgtagt aggattttta tatccccttg cttttataaa cttagtatag
     7261 tacaatgaaa acaaattatc ggttgcatat gcaacaagga agtgattatt tgaaaaaatt
     7321 tttagaaaag catatagact tactaaaaaa aattcctcta tttgaaggaa ttaaaagtaa
     7381 tgaacttgaa gaaatgttgg aatgtttagg tgttgtagat aagacttacc caaaagatag
     7441 tataatattc tctgttggaa gtgaagttac ttctattgga attatgctta aaggaagtgc
     7501 tcatataata aaggaagata ttgaggggaa taggaatatt gtagctgaac tcttgcctgg
     7561 agatttattt ggagaggtat tcgcatgtac tagactacat aaaagtaatg ttacagtaac
     7621 cacaacatct tcttgcgacg ttttatttat aaagtttaaa agtgtaactg gtatttgttc
     7681 atctacatgt gtatttcata atagacttgt tgaaaatatg ctacaattaa tagctgaaaa
     7741 aaatatttta cttcacaata aaatagaatt actttctcgt aaaacaactc gcgagaaatt
     7801 gcttatgtat ttttcaaagc agatagaaca aacaggtagc cacaagttta caatcccttt
     7861 ttcaagaaat gaaatggctg attttttatg tgttgacaga agttctatgt caagagagct
     7921 tggcaagatg cgggatgaag gcttgttatc ttttaataaa aataagtttg aaatcttttg
     7981 ttttccaaac aagttaaata tctaggctta tataatatct gtattcaaat aatattgttt
     8041 tttattagtt attttctaga aacctttata caaaaaagga gtattaataa ttaatgctcc
     8101 tttagctata ctatagatta aacttttatc tatccattga tatcaatggc tattttcatt
     8161 ccaagtttat aggcctttct caataccttg aaattttttt tagtggagtt cctttcaaat
     8221 gctttggttg caactatagt tttgtagtga tttaagccca taccctttaa atgctttgaa
     8281 gtatacttga aatatttttt aaataaaaat gaaacagggg ctgcttgagt atagaccatg
     8341 attaactttt tttgaccaaa cctaggtgta tgtttagact tatttatctc tgttaaagga
     8401 tacagtctat ctaaaagtag ctttgtttga gctgatacct gacatatata tactggcgaa
     8461 ccaattatta tatattcagc actttttata tctttatata tgtcaatcat atcgtcttta
     8521 aatgcacaag taggaacttt tctacagtat agacaacctt gacatccttt aatattcata
     8581 tcatttaaat aatatttctt aacttcaaca ttattatcat ttacaccttt tattatctca
     8641 tcaagtattg tgtctacatt cccttttttt cttggactgc ccataaaggc taatactttt
     8701 accatattaa gttctcctgt ctaatttatt tttctaaatt taatctaaag cacactttat
     8761 tctaacaata tattttatat actttgtatt tgttttattt aaattttata ttacaaaagt
     8821 atagcaaaaa ataattctta acacaattat ttaaaagtaa tatagcaatt taatacagtt
     8881 atttattaaa ttttaatgct cctgtaggac ataccattac acaaggtgaa tccataccac
     8941 acttatatct ctcaatacaa ccattacact tatttgcctt accatcattt ccataaatta
     9001 aggctccata ttcacaagct ttttcacaaa gtttacaccc tatacagttt tgattgtcta
     9061 atacaacgaa tccttcttct tttttaaaac agtctttagg acatatatct aaacattttg
     9121 gctcatcaca atgtatacat gctaatgaaa aatattctac actttcgcct tcttcgattg
     9181 taaatacaca tctaaaaagt tcaccattat ctatatcatt atcattttgg tctatacatg
     9241 caagcaaaca tgctccacat gctgtacaca attctttatt aaatttaaat aaacttttca
     9301 ttatatccct ccatctgtct attcatcttt tcaatcttgc atttacaact tctatatgca
     9361 gggaatccac tataagggtc aaaattatca caaacaatac tatttacatc agcttctctg
     9421 tatccatgat acatatgtat atttctgtat aagactctat cagtaatatt gacttttaca
     9481 tccaaacttc ctgtcttagt agttattttt atataatctc cactgataac atccaattct
     9541 tttgcgtcta aaatggaaat atcacaacta gcttcatttt ttaatattct tgaaagttta
     9601 atgtcatgaa gtcttgaatg tagtgcattt gaaattcttg ctcctgatgt caataaatga
     9661 gtatattcat tatctatatc atcattaaac cctctatatg ttggtagtgc atctaatcca
     9721 aaatctttat atttttcaat aattgtagat tttaattcaa acctagaagt actagtatca
     9781 tacccttctc ttgtatatga acctactata tacggcttaa aatctttaca ttttaatggt
     9841 aaatcacttt ctttaagttt atctacatct acacacgtat ttttaaacat atatcttata
     9901 cttgcttcat aacctgcttt taataaatca tcatctatat tcataacatt tgctaaatct
     9961 gaaagtattt ctacatctga ttttgaatcg tataatttat caataacttt tttggtatat
    10021 gtaatatacc ctcctggata actcttacat tcttctctct caaatgatgt acacgcaggt
    10081 aaaagtatat ctgcatattt tgatgtatct gtcataaata aatctatatt cacaaaaaaa
    10141 tctacactat tcaacgcttt aaaaagtctc cctgaattag gaaacattct agcatttatc
    10201 ccatgacaaa acaaagctct tatttcatat ggtgtctttt cttctatttg cctaggtaaa
    10261 tccatagctt gaaattgagt caccatttca ttccatagag gaaatctttc tccaccaatt
    10321 ctttcattta tatttttagg tagaacttct tttacaaact ctttttctag tgtctggaat
    10381 ccacctatct ttccagcaaa gtaatcttgt tttggtatgt ttccacctat tctatcataa
    10441 ttacctgtta tagcagataa agacatcatt gctctatagt tttgaaatcc attttgatga
    10501 tgaactattg gagctgcact ttcatttata caaaatggac cttgtgacaa catcttaaca
    10561 gcttttacta tattatcttt agaaactcct gtaattctac ttacagtatc taaatcaaat
    10621 aattttacat attctttata ttcattaaat ccatgtacat gcttttctat gtattctcta
    10681 tctattaaat ccttttgtat aaatagattc gccattccaa gagctaaagc tccatcagtt
    10741 ccaggtttaa ttcttaaatg taaatctgct agtttttgag ttgtcggtgt tattctaggg
    10801 tctacaatta taaattttag acctttttct tttaatttta ttagattcac actatttaaa
    10861 aatttagagt agtatggatt aaatccccac cctaaataag tttttgcatt ctccatatct
    10921 tgcattgtaa agtttcttcc agtagcaaca tttccagcca tctgtgctga tgttgaacat
    10981 gaactagatt ctgtaccata attaggtgaa ccaaatgaag atgcaaacct ttgtaaaaac
    11041 ggtctatacc actttccata acccgtaaaa aaagctacat gctttgctcc atattcagat
    11101 tttactctat ttaatttttc tgctatttct ctataagctt catcccatga tattggttta
    11161 aattttcctt cacctctttt tccaattctt tttagaggtg tttttattct atcttctcta
    11221 tagatatatt ctctattgca aagacctttg gtacataaca accctttatt cattggatgt
    11281 tcttttgtac cttctatttt tattacttta ccatctttaa catatgcatc aattccacaa
    11341 tgtactcctg gagaacaaat atcgcataaa gtcctcttta tttctatacc tgtttcctta
    11401 catggtattt tagctttaag ttgtctataa ctatccatat taacattttc ctttcctaat
    11461 aataaataca aattattata cttattatac attcttttta ttcaaatatc atactttata
    11521 ttttatataa tgcaatttct aagaatattt ttaataataa gattttatat ttactaataa
    11581 tctatttata taattatttt tcattcatag tttataagat aattcaataa aaatctaata
    11641 gtaaattgtt atttacacat atattgtaat agataaaagc tattcatatc tgccttaatt
    11701 ttagtcttaa atttagagtg taaattgaat gcatttagat tataatatat acagtgaggt
    11761 aaggtgaata atatgaaaat agatatgata tataaactaa taagaagaaa tacagaacca
    11821 tcaagatggt caagaggtgt aaaatattat actaatcaac tagttgatta tatagacata
    11881 gatattgatg acctacaaaa taatctgttg acatttcgtg gtggagtcaa ttctgaagat
    11941 aattatagct tttattttaa ttcaataact atagatataa agcactttaa gataattgaa
    12001 aatgattgta gttgtgctga ctgtcaatca aagggcaaaa gtatttttat atgtaagcat
    12061 atagttgcta ttgtgataaa aagcttaaaa caaataaaaa ataaaaaaga tgttcctcct
    12121 attctaatag atatattagg tgtagataag gataagcttt ctaatacaaa ttctaaagcc
    12181 agcttagatt taagcaagca actactttct ttgttagaca atactaaaag agaaagggtt
    12241 aatttagatg tattgataaa tgtggattca aataaggcaa ttgctgaatt taaaatagga
    12301 attgataaaa tgtacatttt aaaaaacatt actgaatttg catattcaaa acttaatgga
    12361 tatgaattag attttgggaa gaaatttact tacaacccca acagtcatta ttttgctgat
    12421 gaagatgaga agttagtaga tattatagaa gaatatggga caaatctttc atacaacttt
    12481 tcttcatcac actcaaaaaa atttatggag ataaatgcaa gttctttaaa acaattttta
    12541 aatgctatta agtacaagga ttttaccctt attttttcta aagaggaata tcatccaaag
    12601 atattaaatg atactctacc tgtaaggttt gattttaaaa atatagaaga taaaatagtc
    12661 ttatattcta aagattcact acctgttcca cttactaaaa aaaatgatgt acttttatat
    12721 gatggcaata tctatttatt atcacataaa aaagctcatg attattcaaa aatatatcaa
    12781 gtattatcaa agactaaaga gttaaaattt gataaagagg attctaagga tgttttagga
    12841 ttgctagttc ctagattaaa atctatatct caagaagttc atttagatga taacataaaa
    12901 aataatataa caaaagattt taaatctgag ttctactttg atatggttga tggcaacata
    12961 tgttgtgatg taaagtatat atatgatgat gaagataaaa aatttgtgct tcccaacaca
    13021 caaaaagaag ctattattga aaataaactg acttcttcac aatttacaaa ggaaaataat
    13081 cactatgtgt ttaaaggaac tgaccatgac ctatttacat ttttagattt acaattagaa
    13141 tctctaaagg aatttggaga tatatattat tctgagaagt ttaaattgaa aaaaatatat
    13201 aatgcttcat caattcaagc ttcaatcaat cagactaacc agaattacct agaatttaat
    13261 tttaatatat ctgatataaa tccaaaagag tacaaggata ttttgaaagc ttttcaagaa
    13321 aaacgtactt tttacaaact taaagatgga aactttatag atttatcaga aagagaaaca
    13381 aaagattttt ttgaattagt tgaaaatcta gacataatgg ataaatcaaa aaataataaa
    13441 atccataaaa ataaagctct atatataaat gatgtaatac gtagtaaaaa tttaaagttt
    13501 atagatggta aaaaagaact agacagtata tgtgaaaaat ttagaaactt tgatagcata
    13561 aatttagaaa ttcccacaaa tttaaatgca aatcttcgtg actatcaaat aaatggtctc
    13621 aactggttta aagttttaga ttattataaa tttggtggta tattagctga tgaaatgggt
    13681 cttggtaaaa caatacagac tattgcattt ttattatcat tatccaataa aaaaagtctt
    13741 attgttacac caacatcgct catttataat tggaagaatg aatttgaaaa gtttgctcca
    13801 gatataaaag ttttattgat acatggaaat aaaagagata gagaaaaatg ttttatagaa
    13861 ttagaaaatt ttgatgtaat acttacaaca tatggtactc ttagaaatga tttagaaaaa
    13921 tatagtgaaa taaagtttga ttattgtata atagatgaag ctcaaaatat aaaaaatcca
    13981 gtagctttag taactgagtc tgtaaaaagt ataaatgcag aaaataaatt tgcactcact
    14041 ggtacaccta tggaaaataa tctactagaa ttatggtcta tatttgattt tataatgcct
    14101 ggctatctat atagtaaagc aaaatttcag gaattgttta taaataaaga agataatgtt
    14161 aaaaacctga aaaaattgat aaaacctttt attcttagaa gaagtaaaaa acaagttatg
    14221 aaagaacttc cagataagat tgaaaaaaac ttttttgttg aattgaataa agagcagaaa
    14281 aagatatata gtgtatattc aaaagatata caagataaaa tgaaagataa aaatctaaag
    14341 aaagataaaa tagttatatt ttcttattta actaaactaa gacaactttg tttagaccca
    14401 agtatagtag taaaagatta caataaaaaa agttcaaaaa ttgaaacgtg tttagaaata
    14461 cttagggata gtataaacga aaaccacaaa atattattgt tttcacaatt tacttctgtg
    14521 cttaagaaca tcagtaagga acttgataag tataaaatta aataccatta tatagatggt
    14581 aaaactaatg ccaaagaaag acttgaactt gtagatgagt tcaataatag tatggataaa
    14641 aaagtttttt taatatcttt aaaagcaggc ggaactggtt taaatcttac atctgctgac
    14701 atggtaattc actttgaccc atggtggaat ccatcagtag aaaatcaagc tagtgataga
    14761 gctcatagat ttggtcaaaa aaattcagta caagttataa aattgattgc taaagggact
    14821 atagaagaaa aaataataaa attacaagaa agtaaaaaag agcttataaa tcaatttata
    14881 aatggagaac tttctaatga aggcgtatta aaaagtctct ctgatgagga gataatagac
    14941 ttatttaact aaaatattta atcctacagc ttaactatat aaatttgttc taacatttat
    15001 atagttaagc tacaatacct gaataaataa aatttcatat tgttatttaa tcccaacttt
    15061 acaatccaat gatatctctt tctatacata ttgcatataa aaatgtaata acttgactat
    15121 tagaatattt tgttttaaca atcctatttc atcataataa tgaagtatat gtacactcac
    15181 ctttgtaagt tttgcaatcc attttacagt tttcattatt gtaaattttg gttaaattat
    15241 tcctctaaaa atttctttgc tacatttgaa aaatctaatc cattcgataa aattgcagga
    15301 tgaccacctt tatcaaacaa atacatttta ccatttttaa tcttttcaag aagtgatgaa
    15361 aaagtatttt caaaaaaatc attagatatt agagatacaa actcatcttc tctacttcct
    15421 gttaaaagca cctctggttg catagtttct agaggcttat gaaaaaattt tcctatagtt
    15481 ttataatgtt caaaaatagc ttctgtatca ttatctacaa ctttttccca gttttctcct
    15541 tgattataaa tataaaaagc ttttgcacca tcatcttgct ttgatgcctc acgctctatt
    15601 ttaacatttt gtacaaatga ctctaaagga acttctcctt caaaactatc tgcaattact
    15661 ttattaacta aatcaggtct ttcaagtgca acatttaatg caacaagagc tcctccacta
    15721 ctaccaatta tatttacttt tttataattc attttttcta aaaaacatat cacttgcata
    15781 gcttcatcaa accataagtc agcagaaaac ttatcgacca tttgagattt tccatgacct
    15841 acaaaatcaa ttaatattac tttatattca tctttataaa aatcgattat atcattaaac
    15901 atattagaag aggctgtatt tccatgtaaa aaaagcaatg gtgtaccatc cccaatttca
    15961 ttatagtagc actgtttgtc gttatagtta aaatatgcca tatatttccc cctttaatat
    16021 tcctataagt atatatttat tacttatatt taaaatgact attgtcataa acacttaaaa
    16081 atagtatagc ttagaatgta gctaaagtca acattccttt ccctaacaac ttctattgtt
    16141 tttttgcaac ttcatttcga taacagaata attcatcaat attgatactt ttacttaaat
    16201 atatgatttc atcatcagag agagcaataa taactaaatt atcgtatatc tcttttcact
    16261 ttaaccattt ttttctttat aggtatctcc ttatattttt ttaatatcaa atctccctta
    16321 taatttaata tatctacata tgaacataaa tcttgaactt caattacacc tatatcatca
    16381 ttagttatac cttttatatt actaaatgca ccaactatat ctaaaactct tattttcttc
    16441 ttcttaccag catttaaata taatttaaca acttcacttt ctgtatttag ttttatatct
    16501 ttacttttat aagaattttt atcagctttt tttctctttg atacttcttt taggatttct
    16561 tttgaatatt cttcaaacaa aattttgccc tgcgtaattc tactttgctc tatatctgtt
    16621 agctcattta ttttatatcc tatatacgtt tctatattct ctatatactt ttcatccttt
    16681 tcatttacta tagtaattgc ctttccacta tttccttttc tcccagttct acctattcta
    16741 tgtatatagt tttctttatc ttgtggcaca tcataattta ttactaaaga aatatcatca
    16801 atatgtattc ctctagacgc tacatcacta ctaactaata cattaaactt ttgtttttta
    16861 aagtccttaa tcacaaaaat tcttctctcc tgcgataaat ctgcatgtaa ctctcttatt
    16921 aaaaaaccgt ctttactcat cttattatat aatttagata ctttctcttt agtattacaa
    16981 aatattatca cactctttgg attttcgctg tatatcaatt cttttaaaag gatatacttc
    17041 tctcttccat ttgccttgat tattttgtca tctatttgtc ttgttctttg attattatcg
    17101 atctcatttt cctctatatt tataacactg taatctggca tatacttttc acatatatat
    17161 tttatctctt catctattgt agctgaaaaa agacctacta ttttctcctt aggtaaattt
    17221 aataatattt tttccatttg ttcaacaaaa cctttattaa acattttatc tgcctcatct
    17281 attactaaat actgtatatt ttccaactta atagtacctt tatttatatg gtcaagtatt
    17341 ctgcctggag tagctacaac aatatgaacc ctttgtttta attcagctat ttgctctttt
    17401 ataggttgtt tgccaaatat agcactacat ctcaccttct ttagtcttcc tatatccgat
    17461 atctcatctt ttacttgcaa ggctaattct cttgttggaa ccactataag agcttgaata
    17521 ttattgtaat ctacatttat attctcacat aaaggtattg caaaacttgc tgtctttcca
    17581 cttcctgttt ttgacttaac tactaagttt tgacctttta ataattttgg aataacttct
    17641 ctttgtactc tagaaggtat attatattct aaactcttca acgacttaag tatcttttca
    17701 tttaacttaa atttttcaaa tgtatatttc atatattacc accttattgt atttatctta
    17761 tctatatatt tttctacatt atattattat aatacaaggt tctactattt ttatattatt
    17821 tattagattg aagataaaaa tatatactat ataaacctat agtttagtag caaattaatt
    17881 aaatttttat atttaaatac ctccttgaat atactgataa atcaatattc tcaaggaggt
    17941 atctcttgtc aataatcaaa ctaatctatc ttttgcaagt ttaaattata ctaatatatt
    18001 gtatttttat ttatatctaa tattaaaagg tattaatcaa tacctaaatc accataatct
    18061 cacatattta tatctttatt caatatacct tctgtaatca atcgtgctat ataatcataa
    18121 cctgattcat ttgctttgtt acaatcattc acattatcac agaaaaaact ctcaatctgt
    18181 attgcaactg gatttgtatt tctcaatata tataaatttt ctcttttttt agctcctcta
    18241 ttttcaaata attctccaag tctattagat actcttttag catattctag tccttccttg
    18301 ccatagtaaa gcacttctgt accacattgt gttccatcag ctttatttaa atgtagctct
    18361 actaataaat cgtatttacc actgtttact tttggaactc tatagaaaaa ttcttctctt
    18421 tctgacataa agtgtctttc tggacaaatt attacatcta ctgtattacc ttgacttata
    18481 agcatctcag ctaacatagg agctaatttt ttgttataat tgtattcatc aactatacca
    18541 tttacacctg tacttttacc accttttagt atgctatgac ctactgttat gcatattttc
    18601 atttactttt ttcaccttct tttaagccaa tttttatata atagtaatat aattttaaag
    18661 ttattactaa taataatata ttaattgaag aaggtaaaag ttctccgtga ttatcatttt
    18721 tttaacatat ttgtcttatt ttatcataaa t
//
LOCUS       DAEMAE010000060        18335 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEMAE010000060 DAEMAE010000000
VERSION     DAEMAE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.061"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(85..1458)
                     /locus_tag="KPH93_001643"
     CDS             complement(85..1458)
                     /locus_tag="KPH93_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688470.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKVAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFIPVITGLVVTT
                     IVAMFII"
     gene            complement(1645..2967)
                     /locus_tag="KPH93_001644"
     CDS             complement(1645..2967)
                     /locus_tag="KPH93_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1688471.1"
                     /translation="MISKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDDFQTYVNNNGISADDGDDIAKWNGDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPVVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(2960..3664)
                     /locus_tag="KPH93_001645"
     CDS             complement(2960..3664)
                     /locus_tag="KPH93_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1688472.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(3847..4911)
                     /locus_tag="KPH93_001646"
     CDS             complement(3847..4911)
                     /locus_tag="KPH93_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1688473.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVVGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVENSRKVITSQI
                     EEYVKGAQYIKQNPKLVVQILVITIIQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(4914..5612)
                     /locus_tag="KPH93_001647"
     CDS             complement(4914..5612)
                     /locus_tag="KPH93_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1688474.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNVAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     KNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(5603..6700)
                     /locus_tag="KPH93_001648"
     CDS             complement(5603..6700)
                     /locus_tag="KPH93_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG1688475.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLNEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHINHSTKKNILIMGGGLGLLPKSEQFYRELNGL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDVTLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            complement(7025..8269)
                     /locus_tag="KPH93_001649"
     CDS             complement(7025..8269)
                     /locus_tag="KPH93_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG1688476.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            8597..9385
                     /locus_tag="KPH93_001650"
     CDS             8597..9385
                     /locus_tag="KPH93_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1688477.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            9385..9858
                     /locus_tag="KPH93_001651"
     CDS             9385..9858
                     /locus_tag="KPH93_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1688478.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGITGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(9950..10390)
                     /locus_tag="KPH93_001652"
     CDS             complement(9950..10390)
                     /locus_tag="KPH93_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG1688479.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAAMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(10405..10698)
                     /locus_tag="KPH93_001653"
     CDS             complement(10405..10698)
                     /locus_tag="KPH93_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG1688480.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(10741..11142)
                     /locus_tag="KPH93_001654"
     CDS             complement(10741..11142)
                     /locus_tag="KPH93_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688481.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11375..13495
                     /locus_tag="KPH93_001655"
     CDS             11375..13495
                     /locus_tag="KPH93_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688482.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSRYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINVLPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFSLSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            13720..14886
                     /locus_tag="KPH93_001656"
     CDS             13720..14886
                     /locus_tag="KPH93_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1688483.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLNRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKENDKIMIQEPVYSPFNSVVKNNNRELVISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKYNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFNALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            14911..16353
                     /gene="nhaC"
                     /locus_tag="KPH93_001657"
     CDS             14911..16353
                     /gene="nhaC"
                     /locus_tag="KPH93_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG1688484.1"
                     /translation="MKMNTEKKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLISAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYIQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLINKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTIQRRVD"
     gene            16356..16625
                     /locus_tag="KPH93_001658"
     CDS             16356..16625
                     /locus_tag="KPH93_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688485.1"
                     /translation="MRFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLSI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLLISIIINPRIKVSK"
     gene            complement(16701..18128)
                     /locus_tag="KPH93_001659"
     CDS             complement(16701..18128)
                     /locus_tag="KPH93_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG1688486.1"
                     /translation="MKLIKSISSMLCLSLVLSLSPVAYANEISRTAVENTLVGQDRYQ
                     TAVEVSKKGWNSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLATERVGGSDRYQTALSIAKKIEGLKDISE
                     IAIVNGYTGLADAVSIASVAATNGMVILPVSDTSGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKKQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLSNGDEVEVLDVLKTGWVRIKYNEGIGYV
                     SGSYLTNNKPNNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
BASE COUNT         6304 a         2918 c         2306 g         6807 t
ORIGIN      
        1 ggcttagaat tagtaaaaca attatgataa attatagcca cacatctatt tttaatttgt
       61 acaattaaat atttatttga tttattaaat aataaacatt gctactatgg ttgttacaac
      121 caatccggtt ataactggga taaagtttcg ctttgccaat tctattggac taactccaca
      181 aattgccgct gctgaaataa gaccccaagg aactagacag cctccaccta cccatatacc
      241 actgatttgt cctaatgcag ctaatgcacc aacacttgca tttatagcag ttccaaatac
      301 agaggcaagt gaaccaacta atgaaatccc agaaaatcca gaaccatcaa gcccagttat
      361 tgctcccact acagtttcta tacctgatac ggcaacttta ttaagtggta ttgtttgaga
      421 aagagctatg cctatatctg ataataaacc ttgtgaacct ggtgctaata cttttccaaa
      481 tacccctgtc aagggtgcaa cttcacccat atagaaaaat gctgctattg gtataattgc
      541 tccaaatatt tttatcccaa atacaaaacc atcaattata ttctcacata cattttcaag
      601 actatctttg ccaaaattta taacatttat aattattatt aaaaatactg ctgttccacc
      661 caaaagagct gatgcatctc ctccttttag gtcaaataca tacatagcaa ttatatcagc
      721 aagaaatccc agagctacta aaatagttgc tattttagat tttccattaa actcttctac
      781 ttcttcgctt tcaaaacctt ttagttcttc tctaaacatt cctttgttaa tatctctttt
      841 taatgtataa aatgccatag atatggttac gattcccatc acccaaaaca aaatcatacc
      901 atcatttata acatatgata ctgcaacacc tgctgcacca gctgttatac ttggagctcc
      961 ttgaataaca aagtctgtag acaaggcaag accatgacca aataaattta atgctactgc
     1021 aacaccaatt gctggtagcc cagctctcat agcaacaggt aaaaacacag ctccaactaa
     1081 ggcaacagct ggagttggcc agaaaaacca agaaagaact aacataacaa atccagttac
     1141 aaaaaatgct gtattagaat tttttataac tctcgaaaat ggtctcacca tatactgtat
     1201 agcattattg gcttctaggg ctttagataa tgcaaccata atagctataa tcaagatgat
     1261 tggacccaac tcatttaaag atataataaa agagttaaac actgctgtta tagcaccaaa
     1321 aatgtttttt tcaaagaaca atcccataaa aaaaacacct aatatacatg gtactattgt
     1381 atcctttttc ataatcatag taattaatat aattaccata aatagaatgt aaatataatg
     1441 tgctcctgtc aaaaccatat tattatgtcc ccttttttta agataataat attatatggt
     1501 cttgtaagta aacttgttac ttgcctagag gtacacaata ttgaacatcc cataactaaa
     1561 gtcacatgct tcctgcttca tagaatttta cataagcaat tacacaatat ataatagcaa
     1621 ttacacaata tataatttaa atttttatga ttcttttgct ggaatagata tttcaatagc
     1681 aaattcttta tcatctgaat ttacatttat gcttccatta tgatatgcaa ctattttctc
     1741 acaattcttt ataccaattc caaaactttc tacattctta gaaaaattgc ttactctatt
     1801 tttttgtata attaataatt tagaatcctc tactttaatt aaaaatacta caggtttatc
     1861 tttcctagca tattttatta tattagaaca taaattatca aatatccttc taaattgtgt
     1921 aacatctaca taaatatgat attggttata aagttcattt tctattgtta tatgaaagcc
     1981 tttctcctcc aatgtatatg aactatctaa aagtaattga aaaataaatt cattagcatt
     2041 tattttttca tacccttttg aaacttcctc tccagaatta acaaaatgcg taaaaaggtc
     2101 atctgttaaa tcttttatct gatatgcttt agaccttaca ttttttatgt attgttccaa
     2161 ttgctcagga ctattgtatt tatggtcttt aatcaaatct aaataacaaa ttactgacgt
     2221 taatggagtt ctaacatcat gtgatatcga agttactata tctttatttt tattcttcat
     2281 ctctctttct tttataagtt gattctccaa tgtgtggctc atctcattaa tactagatgc
     2341 aagtgatgat aattcatcat taccttttac tgggattcta tactttaaac tgccactctc
     2401 tactatcttt atacccattt ctattttcct taggtacttt atctttctac taaacaacag
     2461 taaaaatgtt ataataaatg taagtgccat aaatgtaata gaaattgctt ctataaaatt
     2521 ttgaaatcta aatctaaata aagctactac aactatatta gctttaatat cagaaaattg
     2581 tatctcatag tttctagcca tgggactgct ataaaactca ttttcctcac tcttatcagg
     2641 tacttcatta ggatagtaca atgtatcata aaaaacctta tttcctttat atatcttcat
     2701 agaaacaaac caattatcac cattccattt tgctatatca tctccatcat ctgcacttat
     2761 accattgtta ttcacataag tttgaaaatc atccataagt tgatttactt ctcttttaaa
     2821 atatactttt ccttctgata catcttttac tatagattca acagcagagt aaaatggtat
     2881 atttaaaatg aaagccactg cagctgagat tactacatat atcaatagta taactcccaa
     2941 cttagatggc ttaattttgc taatcaatat aatacccctt tccccatgcg gtttttatat
     3001 attgtggatt tttttggtct ttttctaact tttttcttaa atttctaata tgaaccatta
     3061 ttgtattatt tgccgtataa aaatactgct catcccaaat agtttcatat aaattttcag
     3121 atgaaaaaac tttttttcta tttttcaaca taagaattaa tatactatat tctatagttg
     3181 tcaaaaacac aggttctcca tttagtgtaa catcttgagt atctaaattg acacataact
     3241 cttttgtttt taatatattt ggctctcttt ccttttcctt tccctggtat atataataac
     3301 gtctaagtag cgacttaacc cttgaaatta gttcagaata tgaaaaaggc tttgataaat
     3361 agtcatctcc tcctgctgaa aatccaataa ctttatcaga gtcttgagtt tttgcagtta
     3421 aaaataatat tggtgctgtc gttttttctc taatttcatt acatgcaaca aatcctgttt
     3481 ttacaggcat tacaacatcc aaaataatca aatcaatcgt actatctact aagtttacag
     3541 cttcttctcc attcgttgcc ataataactt cataaccttc accagttaaa agtatttcta
     3601 cgatttccct aatctcagga ttatcatcag ctactaaaat tttttttgga atctctttat
     3661 tcatcgccaa gctccttttt ttgctatatt tcttaaatat ttcaaactat ataaaatatt
     3721 atactttatt agcaaaagct tagcaatgtc acacgtttta atactttcta ctcctgtaaa
     3781 caggtttaaa acttcattaa aatgtataca caaattgaca tattaataaa aataatctat
     3841 ataatattat cttacataag caactttttt cactgctttt actttttcag aagattttac
     3901 atgtactact atacatatca atccacttat agctacaaat gcgtaaaaac ttattcccct
     3961 actaagcaac attgccggaa ctaataagtg accagggaaa aatatcttaa ataatgtcat
     4021 aaaactattt tcagaagctc caacagcccc tggcagtggt aatgaagaaa cagcaatatt
     4081 aagcaaggat tgtatcgcta atatctcaaa tacagtatat gttgatagat gaaaagcttt
     4141 gtatacaaag aaaggaacta aaaacatagc tgttatttgt ataatagtta tgactaaaat
     4201 ttggactaca agttttgggt tctgctttat atattgagca cctttaacat actcttcaat
     4261 ttgagatgtt ataacttttc ttgaattttc aacatctttt attattctta acttacctaa
     4321 taaatttgtt attgatatta taaaattgtt tacaagtttt tttgaaaata taattgcaat
     4381 tactccaacg acaagagcaa catttgttat tacaccatat attagtaaaa tgttcatacc
     4441 acttacatta tttaatataa actcacgttc cataatgaac attattccac tatatgccag
     4501 tataactact tggtgaataa caacaatgac tagtagtata agtgaagaat atgatatatt
     4561 tatatcgtct tttttcatat aataaacttg tgctggttga ccacctgatg ctgaaggcgt
     4621 aatagaacta aaataaaatc caataaaagc atatcccata cattttaaaa gagtagtttt
     4681 ttgatttaaa gctttcatac ctaaatatat atttatacct tcacaggcaa catatataaa
     4741 catcattgca aatccaatta gtataaacat agggcttgca ccttttaatg attttatcaa
     4801 tgattcaact gactgccctc taaaaacaaa atatcctgtt atgcccatta gtaaaactaa
     4861 aaaagctaca ctacctaggc atttattttt cttttcagtt ttacttatca taactacatc
     4921 ctttctggtg ttacgaactt taaaccacta gctttaagtt ttggaatagc tatttttaag
     4981 gcttcaatag tacgctcagg tgcacctact gcaccacctg aattttcacc tgcatcatgt
     5041 aaacaaatta tagaattttc ttttgttcta ctcattaatt tgtttataat aatgtctact
     5101 gtactatctt tctcccaatc ttctgccatt acatcccaca aggtcatttt tagattatat
     5161 tttttaacaa atgaatttga aaatatatta gtatgacccc atggtggacg atagtagttt
     5221 acatctacac ctaaattttt cattatattc gtactttcaa taaaatcttt tttaacataa
     5281 ctataactat aaagccatgc atttttgtgt tccaatgaat gtaaaccaac tatatgattt
     5341 tctctcaaca ttctttttat aatttcaggc tctttttcag cattctttgc tactacaaaa
     5401 aacatagctt gtatatcatt ttcttttaac acatctagta atttttctgt atatctctta
     5461 tctggaccat catcaaatgt aagtgcaatt tcattttctc cagccacatt ttttaatacc
     5521 tctttattta atagtttatt atagtatgta ggtataattg aatggactaa aaaaatcaat
     5581 gcaacaacga taattaaacc tactacatac attctctaca cgctccttgt tcatcaagtg
     5641 aaaataaaat attttctaag gtattattat caaattgttt ttttaattct ttcatgttac
     5701 tgctcatttc tttaagagta acatcatcat aaattaaatc ttttatctca tctacatagt
     5761 attttttatt tttaccaagt attctcccaa tttcattgtt taatataaaa ctagcattat
     5821 ctatctcttg ttgtaaaaat ggattaaatg ccaatattgg aagttctgaa taaattgttt
     5881 caaatagtgt tattccacct ggtttagaaa taataaggtc agaatctttc atgtatttat
     5941 atacttcatt ggtgtaacct actacctcta tattttcata tctaccatat agtttatagt
     6001 acattttttt attattacca gttattacag tagtttttac tccttctaaa ccatttaatt
     6061 ctctgtaaaa ttgttctgat ttaggcagta atcctaatcc tccacccatt ataagaatat
     6121 tttttttagt tgaatgattt atatgttcta ttcttttaaa ttcttttttt actggaatac
     6181 cattaacttt aatcttagat tcatctattc ctttaaaaac aagttcttcc ttagtagatt
     6241 tactagctac taaataacag tctgtatttt tactaatcca ttctgagtga ctacttatat
     6301 ctgttatgca tgttattaaa ggaagatttg agttatattt ttccttatat cttgatacta
     6361 attgagaaca aaatggaaaa gttgaaataa ctactgttgg ttgtacttca ttaagtaatg
     6421 tatctaattt atttaaaaaa taatctgaaa aagtaaattt tatatctttc tttccatttt
     6481 cagcacattt ataaaacaaa ttataaagac tacttccccg atttacaaga atagaaaaag
     6541 tcttgtacat tttatctgaa tagttaggca taatgtattc aaatatatct ttaattgtta
     6601 tttctgaatt atcaaatttt gcttttatat cttcacttaa agaatttgat gctgagtaat
     6661 gacccatacc aaattttcct gtaagaatta ataccttcat aagattccct cctcattaaa
     6721 gcttttcttt tcatataatt actatactta taagtataga ctttaaatct ttagaaaaac
     6781 ctagtataat tcttaagaaa tcttaaagtt ttaattaaaa aatacaaaga gaccatctaa
     6841 aaatagagat aaccgtattt tttattattt aaataacaca aataggttcg attacataca
     6901 aaaaaagtgc ctcatgaact aaaaagttca ttttgagaca ccttttttat atctaaatat
     6961 attcaaataa atacacaatc attgtaatta tttaatatgt attttattta gttgttcttc
     7021 ataattaatc cataatatta tattcttcat ataaaggata tttttcagtt aaaccaacaa
     7081 ccaatgttct agccttagat tcttcatcat aagttaaaca taggtctata gcctcagcga
     7141 ttattgccat atcttcttct ttcatacctc tagtagttac agctggagta ccaagtctta
     7201 ttccacttgt aacaagagca ccatttgggt caaatggaat tgtattttta ttagctgtta
     7261 tataagcatc atccaatctc ttttctgctg ctttaccagt tatattttta ttagtcaaat
     7321 ctaatagtat taaatgatta tctgtacctc cagaaatcaa tttaaaatct cttttaataa
     7381 gttcttcagc taaggctttt gcatttttag caacttgtac ctgatattct ttaaactcat
     7441 cacttaaagc ttctttaaag caaactgcct tagaagcaat tatatgctca agaggtccac
     7501 cttgaatacc agggaatatt gctttgtcta tgtctttagc atatttttct ttacaaagaa
     7561 taacaccccc acgaggtcct cttaaagttt tgtgagttgt tgttgtaaca aagtcagcca
     7621 cttcacatgg attttggtga agaccagcag ccacaagccc tgctatatga gccatatcta
     7681 ccataagcaa tgctccaacc tcatcagcta tctctctaaa ctttttaaaa tctatctctc
     7741 taggatatgc acttgctcct gcaactatca ttttaggttt tacttcatgt gctatctttc
     7801 taacttcatc aaaatctata agtccactgt ctttatctat accatattca taaaatttat
     7861 aatattgacc agaaatatta actggagcac catgagttaa atgtccacct tgagataaat
     7921 tcattcccat aacaacatca cctggtttta acatagcaaa atatacacca atatttgcat
     7981 ttgctccaga gtgaggttga acattagcat gctctgctcc aaatattttt ttgattctat
     8041 ctattgcaag agtttcaact tcatctatgt attcacatcc accataatat cttttgcctg
     8101 agtaaccttc tgcatactta tttgtaagat gacttcccat agtttccatt actggaactg
     8161 atactatgtt ttcagatgct attaattcaa tatttctttg ttgtcttttt aactctcttt
     8221 tcatactttc ataaatctca gggtccgctt ttttcaagtt ctcaaacata ttactcctcc
     8281 tagttattat ttaaatttta tttttacaga catttcataa ttttcgtttt tatcaatatg
     8341 ataatttatt attatagtcc tttaccaatt aatagtcaag tgttttgcca tttttccata
     8401 tttaacatag ttactttctt tatttttaag ttaaaatata ataaataata ttgtctaatt
     8461 cttttaattg gatataattt aaatgtgact attttgaaac aaaaaaaaga taatatattt
     8521 atatagatat taaggaatat tacttaaaaa tttgatataa tttcaatatt acgtttttta
     8581 atttggaggt gtatttatga atcaaaatga ggaacttgaa tataagaaaa atattctaaa
     8641 actagctcta tttgtagggg aactgatgat aaaaaatgga gctgaaacat ctagggttga
     8701 agacagtgtc cttagaatat gcaggtcaag agggttttat catgtaaata tttttacaac
     8761 tcctacagta ataattattt cagatgagaa atttgatggc ttttcgttta tgaaaacgat
     8821 tcaatcaaga ggtataaact taaataagat ttctttatta aacagttttt ctcgagaatt
     8881 tgttagtgac aaagagtatg atattgataa tgctattgca cgtctttatg aaatccaaga
     8941 tgtaaaacct tatcctttat ggttatttta ctcttgtaca ggaattgcat ctgcttgttt
     9001 tgcttgtttg cttggaggga attatgtatt aaattttata cttacgttta taatagcaat
     9061 ctttgctgca attacttatg ataaaacgat gaagataagt gcaatatctg cattttcctg
     9121 tttagtatct tcttttttaa ttgcactttc aggagttcta ctagttgaaa taggcatttt
     9181 agatgaccct aaaatgctta tagtcggcgc tataatgcca ctactcccag gagttgcttt
     9241 tataaaagct attagagatt taatatcagg gaatttaata tcaggaattt ctcgtgcttt
     9301 tgatgctgct atgattatta ttgcaattgc ttctggagtt ggttttgtcc ttgatacatg
     9361 gtatagaata ggaggtcctt tctaatgact gatatgtcga tgattttgca ttttttattt
     9421 tcaggtgttg caactgctgg atttgctgtt ttctttaatg ctccactata tctacttctt
     9481 ccagctggaa taactggcgg aattggttgg attgtatatg tttatttatt taattttact
     9541 acaaatgcag tttttgcagg ttttatcgct gctgctttgg tctcagcgtg cagtgagact
     9601 ttagcaagaa aactaaagca accagctatt gtgttcgtaa ttccaggtat tcttcctcta
     9661 ataccaggaa ttgggctata taatacaatg ttgtctttga ttcaaaaaaa ttattctttg
     9721 gctatgtcaa aaggtacaga tgcactattt ctaagtgctg caatagcact tggtgtatta
     9781 gttgtaactt cttttgtaag aacattgaat cttttgaaaa tcagaaaaaa ctttattcct
     9841 tacagaaaat caaaataaaa atataaaaat agcaatgtac ttaaactcta caaatatgta
     9901 ttgagtattt gtttaaaaag cacattgcta ttttttaatc ataattatct taaaggtcaa
     9961 ttaatattct tgttattact ggtataaact ctctactgtc tctaaatttt ttgtcattct
    10021 tctttaagta taaataacag attattgtaa gacaaattcc aactaatcca cttatggctt
    10081 caactccagt tgtcattcca attgccccca atatataata agtccctatg gttcctacaa
    10141 gaagtgctat aagaggtatt atataagcca ttgcagctgc ttttaataca ttcatattat
    10201 ccatactcac ttctacatgg tcaccaactt ttgcaccaat tgtattgtct acttctacta
    10261 atatttcttt tgactctgct gaatttgtag tagcacattt tccacaagca gcacaagctg
    10321 aatgtctttg catttttaac tttgcagttc tatcatcaac aatttccata ataaatcctt
    10381 gttgattcat aactaaccgc ctccttattt tgtaaattta tttatagtat ccataagctc
    10441 ttttatcatc tcttcagttt tatcagcatc atcatgttta atactttctg ctacacatcc
    10501 ttttatatga ttctctaaga taattgtgcc tactctattt atagcagacc ttatagcaga
    10561 tatttgaacc aatatatcaa cacaataacg ttcttgttcc accatctttt gtataccctt
    10621 aacttgacct tctattcttt ttaatctttt tatgagagct tccctctcat tgtttggtgc
    10681 taattttctc tcttccataa actcacctca tactctaatg atgtcttata caattatgct
    10741 ctacttacca acttgtttaa aatataatac agttattatt actataaatg ccaaaattga
    10801 tattgcaata gacctacttt gattcttagt ttgttccata cttaactttc cattcatctt
    10861 aaatcctgcc aatgggaata aaaatataac cagtgcatca attccaagta caacttgggc
    10921 tgcaatagtc catttactca aaaaacagaa tattccgccc acacctaaaa gaacctctaa
    10981 tatatttata aacttatatg taggtttttt tgctatatat tcttcaattt tttccttaca
    11041 ttctgcacga catggatata tattcatatt gtcatcatat tttatagcta atttattatt
    11101 tggtgacttt atatccctat tacagtaaag acattttttc attttatatt ctcctctagt
    11161 attccttaaa aaattttgcc taaattatta tacgctctaa tactattata ccaaatagat
    11221 acaaaaaaca aaaataaaag gaataatttt agctatttct tttacttcaa tgtgtagtct
    11281 ataataaatt tataataata attaaataat attatttaat ctaatttaat tattatttaa
    11341 tctaaaataa gtcaaatttg tatgagaggt taaaatgaaa caaatggaat tagctattgt
    11401 aagtttaaaa aaagacgctg gagaaattta tgaaaatcaa ataaggcaat tcttaggtga
    11461 taatctaaag ataaatttat actcatttga agagggaaat ttaaagtttt ttaaagaaaa
    11521 acttatactc ctatctgctt atttaaaata cgatgaaata gtaaaattat ctcgctatga
    11581 tgctcaaatc attgttccca agttgacatt tgaaaagaac agtattgata tgatatccaa
    11641 attagaaaaa gataaaatta tttatgtata taacttaagt aaagatatgg caatagaaac
    11701 aatttcttta atacatagat taggaataga caatataaat gtcctgccct gctatccaga
    11761 gattgagttt actcccacag atgctgtaat cctaactcct ggtgaaaaaa tcttgcctaa
    11821 gtttaaaaat tgtgaagtta tagatttaaa atacagaata atagatttaa gttgtattgt
    11881 tgaaattgct acaaaaacca aattaaaaca tctaataaaa gatgacctaa taaaaaaata
    11941 cgtagaaaaa ataattccta caagttatag tacaggtgaa ttattgttag atgcaaataa
    12001 gtttgaaaga caatttgatt tgttactatc tataattgat gatggaatta tatgcacaaa
    12061 taatgatgga ataattcaat tctataatca tatggctaga aaaattttat ctataaatgc
    12121 aaatgaaatg attgactcat ttgttggtga ttgcataaaa gacatcaatt ttcaaaacat
    12181 attgaccaat aaaactccat tttttgagaa attgattaaa ataaatcata ttgacataaa
    12241 tttagagatt aaacatatac aattaaatgt atttgatgga tttatactaa agatgacaaa
    12301 gttttctcaa ttagaaaaaa agcaagctaa attaagagca caactagtaa actctggtaa
    12361 tatcagtaaa tacacatttg atgacatatt aggttctagt atacaaacta ttaatacaaa
    12421 aaaaattgct aataaaatgg ctcaatcaaa ttcatctatc cttataattg gtgaaagtgg
    12481 tactggtaaa gaattatttg ctcaatccat tcatagtgct tctaggagaa aagatggtcc
    12541 ttttgtagca gtaaattgta gtacttttca agaaaatcta ctgcaaagtg aattatttgg
    12601 ctatgatgaa ggggctttta ctggtgctaa aaaaggtggg aaaattggtt tatttgaatt
    12661 agccaacaat ggaactatat ttttagacga aattggagaa atggatttaa attctcaatc
    12721 taaattactt agagtcatac aagaaaaaca agtacgacgt attggttcta ataatgtcat
    12781 tgatattgat gtaagaataa ttgcagctac taatagaaat ttaaaagaac ttgtatctaa
    12841 aaatatgttt agaagagatt tatattttag gttgaatgtt cttcccctta aaatacatcc
    12901 acttagggaa agacctgctg atatatttga aatttttggt tcattaaaat atgatattcc
    12961 atgtaatttt agtctaagtg aggaagtaaa agaaattttt aaaatgtata gatgggaagg
    13021 aaatgtaaga gaattaagaa acttaggaga atacttctgt tacttgggaa aagatattat
    13081 agaaatatgt gatttacctg aatatatatt ggatactata gattcaaatt atagcagaac
    13141 cgtttgtaat aaagtaagtg ataatataaa aaagtatcaa ttcaacattg gcaaagacaa
    13201 aaacatcatg aaatacgact ataattttaa aaggtcacta gatgaataca tatttatact
    13261 ggataattta aaaaaggctt atgatttaaa agaaagaata ggtagaaaaa gcttatgtaa
    13321 aatagcttta gaggaaaata gatttttgac agaacaacag attagaaata tgcttttaga
    13381 actacaagac tttgggcttg tagacatact tgttggtcgt ggaggttcaa ttataacatc
    13441 aaaaggagta gaatttttaa aaaatataaa taggtctaac aagttaaaca gttaaaccta
    13501 tttaacctaa ttaattgcat tttcatatat ttaaaaattt attagaaata accttttcca
    13561 caaagtttta agtataatta atatttttat cttatctatg ctctgaaatt gcttaatatt
    13621 aaatattaac ttgtgaataa atatttttta ttatcaatat tggcacgttg tttgcttaat
    13681 attattaaca taaaattaaa attactggag gtatttacaa tgaattataa ttttaatgag
    13741 attgttgata gaagcaataa tttttcatct aaatggtcag agatggaaaa aaagtatgga
    13801 actaatgatt tacttcctat gtgggtggct gacatggatt ttaaggctgc accttgtata
    13861 attgattcct taaaaaatag actagaacaa gagatttatg gctacacaac aagaccagat
    13921 tcttacaatg aatctatagt aaattggcta aataggagac ataactggaa aataaaaagt
    13981 gagtggttaa tttatagtcc aggtgttatc ccagctataa gtcttttaat aaatgaactc
    14041 acaaaagaaa acgataaaat aatgatacaa gaaccagttt acagtccttt taatagtgtt
    14101 gtaaaaaata acaatagaga attagttata agcccattac aaaaactaga aaatggaaac
    14161 tacataatgg attatgaaga cattgaaaac aagattaaag atgtaaaatt attcatactt
    14221 tgtaatcctc ataatccagt tggtagagta tggacaaaag atgaattaaa aaaattagga
    14281 gatatttgtc ttaagtataa tgtaaaaatc atatctgatg aaatacatag tgacattata
    14341 ttaaaaaaac ataagcatat tcctatggca tctatttcta aagaatttga aaaaaataca
    14401 attacttgta tggctccaac taaaactttt aatatagctg gtcttcaatc atcatatgta
    14461 atactacctg atgaaaaaga ctataaattg ttagatgatg cttttaccag aattgatata
    14521 aagagaaata attgctttag cttagttgca actgaagctt catataataa tggtgaatct
    14581 tggctagaat catttttaga atatttagaa tctaacatag attttgctat aaaatatata
    14641 aatgaaaata tgccaaaact taaagttaga aaaccagaag gtacttattt attatgggta
    14701 gactttaatg cacttggatt aagtgatgag gaactagaaa gtatacttgt acaaaaaggt
    14761 aaagttgctt taaatcaagg aaacagcttt ggcattggag gaagtgggta tcaaagaata
    14821 aacttagctt gtccaagaag tatgcttgaa gaagctctta taagaataaa aaatgcaatt
    14881 aactaaaaat atttaaggag aatttgcact atgaaaatga atacagaaaa aaaaatacct
    14941 tcatttagat attccttgtt agtaatgctt gcaataatag ctctaacatc agttggtata
    15001 gttgtattta atgcttctat aactacaatg tttttacttt catggcttat agtagttcct
    15061 gcagccatga aacttggtta tactaatgat gaaatagaag cttttggatt tgaagttggt
    15121 aaagatgcct ttcaatcaaa cttaattata ctttctgttg gtgtactaat atcagcttgg
    15181 atagctgctg gtacaatacc aacagtagta tatagtggac taacaataat aacccctaaa
    15241 tattttctgc ttacaacatt aatcgtatgt tcactaactt ctgttgctac tggtacatct
    15301 tgggggactc ttggaacttc tggtatagca atgatgagta ttggtactag tatgggtatt
    15361 cctcttggtt taacagctgg tgcagtcatt tctggtgctt tctttggaga taagatgtca
    15421 cctctatcag attctacaaa cttagctgca gcagtttgta agacagatgt aattacacat
    15481 atgaagcata tgttttatac cacaggacct gcatatgtaa tatgtatagt tctatataca
    15541 gtaattggat ttaagtattc aaataatact atagactata ttcaaataaa tcaaatcaag
    15601 gatgtattaa attcaaactt tcatattggt ttagtagcaa tgattccaat aatattttta
    15661 ttattacttt tattattaca aaaaccacct attatatcta tactaagttc agctatcatg
    15721 ggacttatta tagcagtatt tcaagaagga gaaaaaatag gtgatgtttt aaattacatg
    15781 ctatcaggat ttactataga tactggattt gtctatgccg ataaactatt aaatcgtggt
    15841 ggaataatga gtatggctga aaccgtttta ctagtatttg tagttttcgt tattgctggt
    15901 attctccaaa aaactggatt cttagaagta ctacttcaac ctcttataaa taaaatagga
    15961 aaatcaagaa ctaaattggt tgggtctact tttatagtga gctactttgc aaatgcattt
    16021 agttcgtcaa tgatgttcac ttctgtattt gtcggaactc taatgtctcc aatatataaa
    16081 gaatttaaat taaaaccaca aaatctatct agaattatag aagatacagc tacacttggt
    16141 ggtccattaa taccatggaa ttcaaatgct gtattctgtg ctcaaactct tggtgtaagc
    16201 ccattagaat ttatcccata ttgtttttta agttggatta cccctataat ttcatttatt
    16261 tatggtgtaa ctggttttac tatgcttaca tacacagatg atgaaattga agagttagaa
    16321 atatttgata ctattcagag aagagttgat tgaatatgag atttgaatac tattacttaa
    16381 ttcaggatat tgcaggtata ttattggcat ttataggact tagaatgtct attattggat
    16441 ttaggatact ttctatgaaa ggaatttcta taaatacact ttcaattgta attaaatact
    16501 gcttatttac catagcagga ctgaatttat taatatctaa gtttggaatt aggcactgga
    16561 tttggagtgt ttgtatgttg ctaatatcca taataataaa tcccaggatt aaggtatcaa
    16621 aatagaaata attattagac ttaaattttt ataaaaaaac tgctttacaa attaaagcag
    16681 ttttttgttg ttacttataa ttaaataacc ggtgtatcag ataggtaatc attacttaca
    16741 taaccatatc caccattata ctttattttt acccatccag tagcaaacat ttctattgtt
    16801 tctactttgc ttccatatga aaggtgacct atcttttcat cttctgtact tggtcctttc
    16861 ctaacattta gaccatcttt ttcttttaca tactttattt ttatattttc attgctatta
    16921 ttaggcttat tatttgtcaa ataagaccca ctaacatatc caataccttc attgtatttt
    16981 attcttaccc atccagtttt taacacatct aaaacttcta cttcatcacc attacttaga
    17041 tatcctataa cagaagcttc tattgtagca tcatttctta tatttacctt atctgtatta
    17101 gttacataca ttactctctt attattacta tttgcctgta aatcttttaa ttctttaaat
    17161 gcattttcat ttcctccact accaacttga gttatagagg tagcattttt attcgaaaat
    17221 acactctttt gtgttgcact taattgttct cctacgatta gtacaggaga attttctttt
    17281 gatgccagta ctcctactgc tagagcatct ataagttgat tttgcttttt cattccatct
    17341 ttggctacaa atacattatt taatttatta gatgtataaa acttttctat cactttacca
    17401 ttagtttcat ttctatctgc tccacctatt ctttctgaat taggtaaact ttgttttatt
    17461 ttatctgata cagcattagt agagcctatt atgtatgatt tatttatatt cttagattct
    17521 ataaaatctt taaagctaga tataccagat gtatcagata ctggaagtat aaccattcca
    17581 ttagtagcag ctacagaagc tatactaact gcgtctgcaa gacctgtata cccatttact
    17641 atagctattt ctgatatatc ttttaatcct tctatttttt tagctatact caaagctgtt
    17701 tggtatctat cactaccacc aactctctca gtagctaaat tattagattc taactgtttt
    17761 tgcacatcat ttgatacaga actagataga ccaattatat aaactttttt aactcctaat
    17821 ctttttaatt ctttctcagt ttgtgaattt agattatttt tttctgtcaa taatatagga
    17881 gcatttttta acttagcaaa cggtgttaca gccaatgcat ctacaataga ttcactattt
    17941 acaactacag cctcatttgc actattccat ccttttttgc ttacttcaac agcagtttga
    18001 tatctatcct gacctactaa agtattctca actgctgttc tacttatttc atttgcatat
    18061 gctactggtg ataaacttaa aactagagat aagcacagca ttgatgatat tgatttaatt
    18121 aatttcatct tgactccccc taaaattata gtatataaat gtttcctatt taaaatagta
    18181 taacataata atttaagtaa catcgttatt ataaaatatt atttttctta tctaataagc
    18241 ttttattctt acttgacatt aatgctttac tcatcaaatg aattttttat ataactaaag
    18301 tttgtaacaa catttcctta gttaatcatt taaag
//
LOCUS       DAEMAE010000061        18228 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEMAE010000061 DAEMAE010000000
VERSION     DAEMAE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.062"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            273..1553
                     /locus_tag="KPH93_001660"
     CDS             273..1553
                     /locus_tag="KPH93_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG1688487.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARTNALFDYIHQNKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRIQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSNSLHELQLSIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1816..3207)
                     /locus_tag="KPH93_001661"
     CDS             complement(1816..3207)
                     /locus_tag="KPH93_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG1688488.1"
                     /translation="MYKLVDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(3218..4438)
                     /locus_tag="KPH93_001662"
     CDS             complement(3218..4438)
                     /locus_tag="KPH93_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG1688489.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFINKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSVPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4452..5240)
                     /locus_tag="KPH93_001663"
     CDS             complement(4452..5240)
                     /locus_tag="KPH93_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1688490.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVRDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGSADELSFALVNKLEELKLV"
     gene            complement(5257..6531)
                     /gene="larA"
                     /locus_tag="KPH93_001664"
     CDS             complement(5257..6531)
                     /gene="larA"
                     /locus_tag="KPH93_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG1688491.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTALSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMIAACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMATSIVGKKSKVVVIPDGVSVIVK"
     gene            complement(6649..7362)
                     /locus_tag="KPH93_001665"
     CDS             complement(6649..7362)
                     /locus_tag="KPH93_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG1688492.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEIIELKHIIDEMRVETNKKDNNRVLVLLDQKFHNKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7487..8746
                     /locus_tag="KPH93_001666"
     CDS             7487..8746
                     /locus_tag="KPH93_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG1688493.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRLVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            9014..9580
                     /locus_tag="KPH93_001667"
     CDS             9014..9580
                     /locus_tag="KPH93_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1688494.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9781..10986)
                     /locus_tag="KPH93_001668"
     CDS             complement(9781..10986)
                     /locus_tag="KPH93_001668"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG1688495.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SMDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRNSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRFLEVVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(11089..11195)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11287..11655)
                     /locus_tag="KPH93_001669"
     CDS             complement(11287..11655)
                     /locus_tag="KPH93_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG1688496.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11707..12984)
                     /gene="obgE"
                     /locus_tag="KPH93_001670"
     CDS             complement(11707..12984)
                     /gene="obgE"
                     /locus_tag="KPH93_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG1688497.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGVGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(13095..13385)
                     /gene="rpmA"
                     /locus_tag="KPH93_001671"
     CDS             complement(13095..13385)
                     /gene="rpmA"
                     /locus_tag="KPH93_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG1688498.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13399..13716)
                     /locus_tag="KPH93_001672"
     CDS             complement(13399..13716)
                     /locus_tag="KPH93_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG1688499.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDAHIKDAQLLLNHFQLAVEEIKREYPKNIKI
                     LKK"
     gene            complement(13731..14042)
                     /gene="rplU"
                     /locus_tag="KPH93_001673"
     CDS             complement(13731..14042)
                     /gene="rplU"
                     /locus_tag="KPH93_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG1688500.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(14143..15564)
                     /locus_tag="KPH93_001674"
     CDS             complement(14143..15564)
                     /locus_tag="KPH93_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG1688501.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRSIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKGYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15582..16283)
                     /locus_tag="KPH93_001675"
     CDS             complement(15582..16283)
                     /locus_tag="KPH93_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG1688502.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPNGIDFIKAKEID
                     RKTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16285..18129)
                     /locus_tag="KPH93_001676"
     CDS             complement(16285..18129)
                     /locus_tag="KPH93_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG1688503.1"
                     /translation="MNKVDLKKILKKVEKPARYFGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENMMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5811 a         3309 c         1939 g         7169 t
ORIGIN      
        1 tttctagtat ggtaaattgt gtatttcact aataatatat taacaaatta acttaatgta
       61 gattattctt tcccatttgt tataataata taatataaag agctaataaa gttattttaa
      121 taacatttaa ctagtttttt atgtaggtaa catttctaca tttttataaa tttttttacg
      181 ttttttaatt ttataattaa aaatttaaat tttatacata ttataataaa ttgtgatata
      241 tataaaataa ctaaagataa aaggagtatt ttatgaacat acttggatta atagttgaat
      301 ataacccatt tcataatgga cacatatatc atttatctaa atctatagaa aagacaaatg
      361 caacacatac tgtagcaata atgagtggaa attttctaca aagaggcgaa ccagctttat
      421 ttgataaata cactagagca aaaattgctg tcgaaaatgg tgtagactta gttatagaat
      481 taccaacttt atttgcttgc caaagtgctg aaattttttc tcatggtgct ataacaattt
      541 taaattcact aaactgcata aattctatat gttttggcag tgaagaagga aatacagaaa
      601 ttctttataa tatagctgaa attttagcaa atgaaccaaa tgaatttaag ttactcttaa
      661 aaaagtattt agatgatggt ctgttgtttc caacagctag aactaatgca ctttttgatt
      721 atatacatca aaacaaaaaa aataatttta tacaaaatta ttcaaaagaa caattatcaa
      781 aaacattaaa ttcatcaaat aacatactgg gtatagaata tataaaaaac ctcatccatt
      841 taaacagtag tataaaacca tttactatat caaggattca atcagaatat aattccgaag
      901 aaattgagag tgatatttgt tctgctactg ctataagaac ttcacttaaa gaaaatacag
      961 acctttttca tttaaaaaat gttattccag aaaaaactta taacatccta aatgaaaaaa
     1021 tagataatgg ttttttacct atgtttgatg acttcttctt tgatatttta aaaacaatta
     1081 tacttagaga tatggacact ttaaaagagt attttgaagt aaatgaaggc atagaaaaca
     1141 gaatctataa agatatcttt acatctaact ctctacatga actacaatta tcaattaagt
     1201 ctaagcgata cactttaacc aaaatcaaac gaactctaaa caatatattg ttaggtatta
     1261 aaaaaagcga catgcaactt ataaaaaaca tagatactat accttatata agaatacttg
     1321 cttttaatga taaaggtaga gaaattctaa aagagataaa aaataaatct gaaataaaaa
     1381 taataaacaa attttcaaat atatcatttt cattagatga tacgattttt aaaacactca
     1441 taagttatga tataaaagct acaaatatat acaattcaat atattataaa aacaataaag
     1501 atttagtaaa aggccctatg gatttttata ctcatccaat atatgtaaag tagttttttt
     1561 aaataactat atttataaaa accattttat taactcttta ttatataaga tacaataatc
     1621 ttactttatt tatgtacaca tgcttttagg atgacaacat caacttaaat attatgttac
     1681 tttctaaatt taagtaaata ttgccatcct aaaaatttta caatttagct tcagatttaa
     1741 aagtaaagtt taaaatatta catttaaacc taatttaata ttttttgttt tgtaaatatt
     1801 attaaattaa tatttctaat atattttccc tggatttaaa atatttttag ggtcaaaagc
     1861 taactttata tttttaatta acatcatata agcatctgaa tttgattcat gtaagtactc
     1921 ttttttagca tatcctatgc catgctcacc agaaacttga ccactaagtt ctctagattt
     1981 tttatacata tagtcaaaag tttcttttaa tcttattttc catgtattat catccatacc
     2041 atcttttaaa atatatatgt gtagatttcc atctcctgca tgaccaaaac tttttattct
     2101 tatttttaat ttgtcttgaa gctcgtgagt gtatcttata aactctgcta tcttatctct
     2161 tggaacaaca acatcacatt catccatttg tgtcgtagat gcctttatag cttctaaaaa
     2221 tgcacctcta gctgaccata tagaatcgtt tctttcttgt gtatctgata taaatacatc
     2281 taatgcacca ttttctaaac ataaattagc tactttttca tattcttttt ctatatcttc
     2341 agtgctattt ccatcaaatg taagtaaaag ataagcatct gatgtattat ctggaaattt
     2401 tttacctaaa aactcttctg ctgccaatat tacatctctt tccataaatt ctatagccgt
     2461 tggtattgat tttgatttta ttattttagg aacagtttct attgccattg ataaatctgg
     2521 aaatggtata agcaaactta tacttttctt tggtaaagga agcaatttta atatagcttt
     2581 agtaactatt cctaaagtac cttctgaacc tactaataag tcctttatac tatatccaga
     2641 actattttta accacctttc cacctacatt tataatttct ccatttggta gtacaacttc
     2701 aagtcctctt acataatctc ttgtaacgcc atattttact gccctcatac cacctgcatt
     2761 agtgtttata ttacctgcaa ttgtagcact tttttcacct gggtcaggtg ggtaaaataa
     2821 atctctatct tgaacatatt gacctattgt catcaacaaa acacctggct ctactgtcaa
     2881 tgttaaattt tcataatcaa cttcaagtat tttattcatt ttacatagat taatcattat
     2941 tccaccattt atggcaacag cagcacctac aagaccagta ccttgacctc ttggtgtgac
     3001 tggtatatta tttttatatg cgtatttcat tattttagat acttgttcag tttcaagtac
     3061 atttactaaa acttcaggat acttctcaat ccctccaagt tcatcatgtg aaaaatcttc
     3121 atttatatca cttccaacta atacattttc ttctccacaa gtatctatta aaaaatctat
     3181 atctttttta tcgactaatt tatacatttc tatcccccta tttagctgct gatgccatat
     3241 atttttgatt attatctttg ttattttcta ttttttcaat caatttagga ataatatcat
     3301 aaatatcacc tattaaagct agatgtgcta catcaagtat aggtgcttta tcatctttat
     3361 ttattgctac tatatagtca gcaccctgca ttcctgcaac aaattgaact gctccagata
     3421 caccacaagt tattatcaat ttaggtttta cagttctacc actaagacct atttgttttt
     3481 tagcatctat ccaacctgct tctattacag gtcttgttcc tgccacttga gcatttaact
     3541 tatcagctaa tttatatatc atctccatat cttcttgttt ttttattgct ctgcttgcca
     3601 caactatcac ttcagcttct tccagaccaa cttctttatt tttttctttt acactcagta
     3661 ccttaatttt agattttagt tctttttttt ctactttaca taaggttatc tttcctgtag
     3721 tattctctat tttttctggt actgaaaata ttttatatct tacagtggca aattgtggtc
     3781 tattattagg tgtatttata tgagccatta tatttccacc aaatgctggt cttatctggt
     3841 ctaaatcagt atttgattga atatcaagta ttgtacaatc tgctgtaaga cccgttctaa
     3901 atctagcagc aagtcttgga gctaatgacc tacctaatgt agttccacca actaacacta
     3961 tcgttggttt tattttattt ataaagtttt ctattgcttt tgaatatggt tctatcctaa
     4021 aatctttaaa ttcatcttca tcatatacaa atacttcatc caccccataa gaaatcaaat
     4081 tactgcactt atcttttatg tcttttccta caaatacaca atacacaggt tgttttattt
     4141 ttgatgccat ctcacgagct ttacctataa gctcgtacgt tacaggatgt atatttccat
     4201 tatgatgttc aacatatact gcaattcccc tccattcatc tttattaatt tgtactttac
     4261 tactctctat aaattcaaat gcgccttttg gtccactttt gacacacaac ttgcacatct
     4321 tacagccagc acttatttct actatacctg acttttcttc tatagcccca aaaggacata
     4381 tttctaaaat tctctttaag tcagttattt tatcttgatt tacaactatc tttgacattt
     4441 attactcccc cttacaccaa ctttaattcc tctaatttat ttacaagtgc aaaacttagt
     4501 tcatctgcac ttcctcttac aatattttta tctgtatttg gctttggagg aaatattctt
     4561 acaacttgag taggagaacc attaagtcca tagttatttt catctttatc ttcaaagtca
     4621 tctaagctat atacaggtat ttctctatct ttagtattaa gttttaattt aaatgaaggt
     4681 agtcttggtt gaaaaatatc tttttctaca gttattaaac atggatattc aatctcacat
     4741 acttgtaaat catttggcat atctatttca acaactatac tttcatctct aacttcaatt
     4801 aattttgtaa cattagtaac atgaggtata tctaaaaatt cagctacttc aggtcccacc
     4861 tgtgctgtat caccatcagt agtttgtttt ccacaaatta ttaggtctac ctttcccatc
     4921 ttcttaatac cttgtgatat ggtataggaa gtagccaata catctgctcc tccaaatctc
     4981 ctatcactta ttaatgcgcc ttcatcagct cccatcataa aactttcttc aattaccttc
     5041 tttgcctgag gaggacccat gctaagtact tttagattcg ctttcttctc ttcctttatt
     5101 ctaagagctg tttctaaagc atataggtca tatggattca tttttgaatc tactccatct
     5161 ctttttagta ctcctgtagt ttcatcaact tctactgaag tcgttccagg aacttgttta
     5221 atacatgtca aaatttccat aaagtacact ccttttttat ttaactataa ctgatactcc
     5281 atcaggtata actactactt tgctcttctt accaactata ctagttgcca tttctaaagc
     5341 ttctttaaaa ttaaatgcat gtttcatatg catttttttt atcatttcag gattacactt
     5401 atccgttacc attattacag tatatttaga taatattctc gctagtatct gaaattccca
     5461 ttggtctgga atcgtatcta ctctatcgac acttctaatt ttatctaaaa gttgttttgg
     5521 agaatctgca tttgctacat tttcataaaa agattgacca ccatgaccat cattacatgc
     5581 tgcaatcatt attattactc caccgtcttt acaggtagct tcagctgctg tcatcccttt
     5641 aacagattga taaatatttt ggtctagtgg atatccacca tttgtagtta caacgatatc
     5701 agattttata gaatttactt ttgataacgc tgttacaaat ctacatcctt tagtatgtgc
     5761 tttttcgaca tcaccagcaa aggaagcaat tattttttta tctttatcta ttacaacatt
     5821 caatatgaat gctaattttg caactttagc tgcatatacc atatcctcat ggataggatt
     5881 attattcaat ttcccagttc ttgaattatc actatctata aagtcactac agtgattgta
     5941 cattatagtc tttgcagatg cgattcctgg aagtacgctt tttctgccac ctgaaaaacc
     6001 tgcaaagaaa tgaggttcta taaaaccttc tgctattaaa agttctgcct catatgctag
     6061 tttgtttata tataaatctc cacctgaagg taatatccct actttacaca tagatttatc
     6121 atccgtagat atatgcataa ctatatcttc attatctact atttcttctc ccattttata
     6181 aattagctct tctcttgtac ttggcctatg aaatccagtt gccactataa tcttcacatc
     6241 aatttctgga ttaacattcc taatccttct taatataatt ggcatagtta ttctacttgg
     6301 tacaggtctt gtatggtcac ttgtaattat gactacattc tttttcccct taaccagttc
     6361 ttccagagaa ttagagccta taggattatt catagattct tcaactatct cttcttgagt
     6421 cttatgttct atataattat ccgattttga ctctaaaaca ccgagaaaac gttcatcagg
     6481 tatttctaaa atcattcctt gttttgaata cggtactttt aactttacca tttttaatct
     6541 cccattataa taaactataa aattggtatg tggtcatacc agttttacat ttatatgata
     6601 gtaccattat ctaccatttg caagaataat ttattttata acaaactttt aattttcatt
     6661 ttttctataa tttttactta taaaatccat atgttccttt gctttctcac atgcaagctc
     6721 tacatcattt tctacaactg cattgtatat agcttcatgc tgtttaagca atatattttc
     6781 attaaaaggt tctgcgatta ttttttctct tgcatcttct ataaatccat caaataattt
     6841 tgatgctgtc ataaaaagac tttgaattaa ataattttta cttaatgttg ctagtttatt
     6901 gtgaaatttt tggtctaata aaaccagtac tcgattatta tcttttttat tagtttctac
     6961 tctcatttca tctattatat gtttcaattc tataatttca gaagaagtta ttctctttga
     7021 agcaagtttt gcaatctcaa tttctaggat tattctaagc tctaatatat cttcaagtgt
     7081 accattatta agtttaaaaa tcatagataa aggttctatt aaagtttttt ctatatttgt
     7141 acatataaaa tttccttcac cctgtctgct ttctatcact cccatagtct ctaataccct
     7201 aatagcttct cgaatggaag ttctactcac attcatttgt tcagacaatt ctctttctga
     7261 tagtaattta tccccctttt ttaactctcc atttagtatt ttatattgta tttgctcaat
     7321 tacttgttca taaacttttt tactagctat actgttaaac attttttcct ccaatatgat
     7381 aatcgttgtt gtaatatata tctattatat atcttaagcc attacattca tatattttta
     7441 ttacttttaa tataatatct tataactcaa atttataggt gatattatga aaaaaaataa
     7501 aattattgct ttttttgctc cagctatcat agttggagtt ctagttttag gtattatcct
     7561 atacccaaat gaagctataa aatctgcaag agagggcttc tctatatggt ctaatgtttt
     7621 agttccatca ctactaccat ttataattgg agcaaactta attgtcgatt taaaaatagt
     7681 ggatattata gggtttatca tcaatcccat aactagactt gtgtttaatg tttcaggaaa
     7741 gagtgcttta gcttttgcta tatcaacagt atctggttat ccagtaggag taagtttggc
     7801 ttctgaattt aggagtaatg gacaaatttc taagcatgaa gctcaaagac ttgtgtcttt
     7861 ttgttctaca tcaggacctt tattcataat aggttcagtt ggtacaggta tgttccaaaa
     7921 ttcaaactta ggttatttaa tgattctctg ccattatcta ggaactatat tggttggtct
     7981 tttctttaga aattatggta atgaaaatct tccaaagaca aaattggatt taaaaacaaa
     8041 tataaaaaat gtaatcaaca ttagaaattc aagtgggaat ggtttttttg ttttatttgg
     8101 aaatgctgta tttaatggag taaatacatt acttacagtt ggtggatttg taattgtttt
     8161 ttcagtagta tttaaaatac tatctctttt taatgtaatc tcattaatag catcagttat
     8221 atatttgcct ctttcatttt taggtgttag taaagaactc tgtcaagcat ttgtaagcgg
     8281 tctatttgaa attactatag gatgcaataa agtatctagt ataacaagtt ctccagaaat
     8341 tttaagagca tctttagcta gctttttgat aggatttagt ggattatcca tcctagctca
     8401 atgttgtaca tttctatcaa aaacggacat agatttaaaa ttatacatac taagtaaatt
     8461 tgcgcatgga gttttagctg ctatctttat atttatactt tatccaattg caaactctgc
     8521 tgtgttggtt tctagctttg ctgatacata caacgttata tataataatt taatctggtt
     8581 ttattatctt agctattatg atacaattct tcaaattgta gtaataattt atctaatctc
     8641 agccgtattt attgcaaaaa aacatatgaa aactttatct acaaatggga aactaatgcg
     8701 atataaaaat acttttttca ttaagaaatt ttcacgaaaa aaataaaaat aataaaactt
     8761 aactaaatac ttccattttt ttattatatt tgtcataatt ttaataacct ttttaattag
     8821 tttaacagca tagttatttc atcacaattt aataatttta ttagaaatat ttttcaaaaa
     8881 attctcctta atcacatata tcacacttgt ataagtttac tggtaaattt ttccattata
     8941 taatatttca tttatattat ataataaata ccgacctaaa cttaccaaat caaactaaga
     9001 ggagtggttg aaaatggcaa caagacctat agaaatagac gaatataaaa agataatgga
     9061 actattacat acgggcttta cttatagtga aaatggcgtt gaaaaaagat ttagaaaaaa
     9121 tcctaaagtt gcattggcac taatgttaga agctaattta ggtctaagaa tatcagatat
     9181 tttaagatta aagataggta attttaaagg taatatgttg gaaataaatg aaaaaaagac
     9241 gggcaaatta cagtatagac cagttaataa aaatataatt gagtccataa atgaacatgc
     9301 aagaaagtat aatcttaaat caaatgacta cttagtaaat ataaagacaa aggctattca
     9361 aaaacagtta aggataattt gtaaacactt aaatctgtat aatataagta cacatagctt
     9421 tagaaagctc tatgctacta ctcaatttga aaagagtaac aataatttag agttggttaa
     9481 agaactttta aatcacagct ctgtagccac tacacagcga tatataaggg taactcagca
     9541 agctattaat gaagctagtg aaaatttctt tattggttaa ttataacgta tacatttttc
     9601 tattatctta ttaattcacc agatattaaa aaagagtgtc tcaaaatgaa ctttttagtt
     9661 catgagacac tcttttttta taaaatcaaa tatattttta ttatttcaat actggaaaaa
     9721 aggcttatct atctattttg agacacccta ttaaattttc ataactcttg cataaacata
     9781 ttaatctatc ttcaataatt ttttagctga ttcactataa ttatttcttt ttacaacttc
     9841 caaaaatcta tcaatctcat gttctgactt tgcacttaca aaaatcgcca attcttcatc
     9901 atctataata tcataactat ctacatattt atctaaacat tctaaaattc catctttatt
     9961 attataccca ccaaatctaa ctactgcact acactcatct tctataagtc cacttaaatc
    10021 taattttcct tttgaaaaag attttatatt tgaatttcta ttatctatta tatctataat
    10081 atctgcataa acagcacttc ctgttgcacc tcttccagct cccttgccta caaaggaaag
    10141 ttcacctact gaatcaccta ctaaaataac tacgttgaac tcattatcta tctttgaaag
    10201 catattatta ttatccacta atgctggtct aacaaatcca cttacttctc ctgattcata
    10261 cttacttcta gcaaccaatt ttatcttaca atttaatttt tttgcatatt ctatatcttt
    10321 ttcatccatt gaagttattc cttctaataa taaatctttc caatatacta tttttccata
    10381 agataaagta gacaatatag ataactttct agcactatca tatcctaaaa catctgcatc
    10441 tggattaact tctgcaaatc ccaactcttg tgcctgtttt aaagcgacat catacggtaa
    10501 attttcatca tacatctttg ataatataaa attacaagtc ccatttaaaa tagcgtttat
    10561 gctctttata ttgttgcctt ccagtgattc tatcaatggt tttaaaactg gaataccacc
    10621 tgccacagat gcttcaaact tgatgctaac attatttaag ttagctaatt ctataaggtc
    10681 actacctttt tcagccagta aatctttatt agctgtcact acatgtattt ttttatttaa
    10741 cgctcttgtt acataatcaa aagctggatt taatcctcca attacttcaa ctagtatatc
    10801 aatatccttg tcaaatatat cttctatatc agtagttatc ttatcaaaat acttcttata
    10861 cttgtgtttt tctttatttt taactaaaat tccaacaact tctatatctc tttttgaagt
    10921 attattttct attaaatcaa ttaatccact tcctactgtt ccatacccta atattgctat
    10981 cttcactgta acataccccc atcattttga ttatattaaa ttatttatat atataaatat
    11041 accaataaaa aaagcttgag tataaactca agccatatga cgggtttata ctcatctttc
    11101 agcataaatg ctgcaggatt tagcaccaat gtataattaa atatactggt tgccgggctt
    11161 cacagggcca gtccctctgc cactcttgat aagattgcta atttaaattt atgtttaata
    11221 gtataataca atttcttcca attgtcacta tttttttaac ttatttccct aaaaggatat
    11281 aaactattat ctaacagctc ttttagttgg tttacccttt tttgtaacat tattattttt
    11341 agtctttgct ttcgcctgtt catgtcctgg aaaaagtatt ttaggctctt caatattcct
    11401 cttgtaaata gtaaatttaa aaccaatagc ctgcacaaat tcagctctta aagcttcaca
    11461 tactgcatta gcagtttctt tagtatcaag acctgcattt tctaatatag taacttttac
    11521 taattctcta gttcttaaca taccatctaa ttgctctaag aaactctctg taacaccttc
    11581 tttcccaatt tgtgtagtag gctttaaagt atttgccaaa gatcttaaat acgccctttg
    11641 ttttcctttt aacatattct cacctctata attctatact taaattatct gtaatctata
    11701 tttaaattaa ttgtaaaact caaattctaa ttcataaatt ttaaccacat ctccatcttc
    11761 tattcccatt tctctaagcc tatcaaaaac accttgactc tccatagctt tttggaaata
    11821 ttgaagggat tccatatcat caaaatttac agaatacatt attcttctaa gtgctttacc
    11881 tgtaactaca tacacaccat cttcaatatc tatacttaac tcttcttcag taccaatatc
    11941 aagttctggt ctatacattt cttcttctga aactagctcc acatcttcta cttcttttag
    12001 cattttagaa acataggcta ttacttcatc tatgccttga cgagttgctg ctgacatttt
    12061 aaatactgta tatcctctgc cttctaattc actcttaaac ttctcaaact tactctcatc
    12121 ttctaatata tcaaatttat ttgcaactac aacttgtggt ctctttgaaa gtttttcatt
    12181 gtacaattta agctcatcat ttatcttgtc aaaatcttca atagggtctc ttccctctaa
    12241 tccagatata tctacaatat gtatcaatac ctttgttctc tcaacatgtc ttaagaactc
    12301 atgacctaat cctacacctt cagaagcacc ctctataatt ccaggtatat ccgcaagtac
    12361 aaaactatct ccaaacttag tttgaacaac acctaaattt ggtgtaagtg ttgtaaaatg
    12421 atagtttgca attttaggtt tagctttagt tacaactgat agaaatgttg atttacctac
    12481 atttggaaat cctaaaagac cgacatctgc tatcatttta agttcaagag ttatccacct
    12541 ttcttctcca tctgtaccag attttgcaaa agctggagct tgtcttactg catttgcaaa
    12601 gtgttgatta cctttaccac ctataccacc ttttgcaact atagctgtat ctccttcttt
    12661 ttttaaatct gctagtacaa gacctgtagc ctcatctctt attactgtac ctgctggtac
    12721 ttttaatact aaattttctc catttttacc agctcttttt cctttgcttc cgtcgccacc
    12781 attttgagct tgatattttt tctggtactt aaagtccatt agagttctaa gccccaaatc
    12841 aacttcaaat atgatgcttg ctccacgacc accatctcca ccgtctggac caccagctgg
    12901 aacatatttt tctcttctaa atgctaccga accatttcca ccatttccag ccttaacaaa
    12961 tattcttgcc ttatctataa acaaattctt cacctactta attaaaaact tttaattagc
    13021 attaccaaaa ataccattat aaattcatca gtattataag aaaaaggagt gcctcaacac
    13081 tccttttatg tatattactc agctattgct actggatata tactaacttt ttttctcttc
    13141 ttgtcttttc tttcgaattt aacagtacca tcaactaatg caaataaagt atcatcgcta
    13201 ccttttccaa cattagttcc tggatgtatt ttagttcctc tttgtctaac tattatactt
    13261 ccagcagtta ctaattgacc atcgaatctc ttaacaccta atcttttaga tatagaatct
    13321 ctaccatttt tagaagaacc tacccctttt ttagatgcta ataattgtaa attcatgttt
    13381 aacatatgga gtcaccccct acttttttaa aatttttata ttttttgggt attcccgttt
    13441 aatttcttca accgctaatt gaaagtgatt tagtaataat tgtgcgtctt ttatatgtgc
    13501 atctttaact atacactcta tatgaccttc ttctcctact actttttcaa attcaatctt
    13561 ttgtaatttg tctaaagaat ttataactgc aatagcatta gatgtaactg cagcacatac
    13621 tatatcatag cctatttcag caaaatcagc atgtcccttt aaacaaaaac cttgcaacaa
    13681 gaaatcatca ttataataat atttaacctt tatcataact taaccaatta ctaagcgttt
    13741 attttttcta caactatctt agtgtatgat tgacgatgac cttgttttct tctgtaatct
    13801 ttttttgctt tatacttgaa aactataact ttcttagctt taccttgttc aacaacttta
    13861 gcttgaacag atgctccttc tactactgga gaacctaatt ttaattctcc atctttagag
    13921 caagctaata cttcgtttaa agtaacaaca tcacctgcgt tagcttctaa tttttcaacg
    13981 aataatacat caccttcaga aactttatat tgctttccac cagtctttac tatagcgtac
    14041 attaaataca cctcctcggc ctcaaactcg ccagtataag gtacttcatt tgaagattta
    14101 aacctttacc gtgcggcatt acaattatta attataaaat atttacattt tattgtcaac
    14161 tattttatcg aaaataacat tggttttatt gtgctttaat gtactatttg cgtttaaaga
    14221 aatctttata ttatattttt caccaatttt atcaattata tccatataat atccttttat
    14281 ttgttcaaaa ataaaatcat taaattctac cattatgttt ttgtaaacag tatgttcact
    14341 tattctcata atttcttttt caatactatc tagtatataa tgtacagatt ttattgtttg
    14401 ttcaccatca caacatggac aagacattaa ataatactta tcaatggatt ctttttctct
    14461 acgcctagca acttctacta aaccaagttt tgtcatacct aatacttcag cttttctttt
    14521 atctttatta aattcttctt ctaggatgtg taaaatctcc ttttgatgct tctttttatg
    14581 catatcaata aaatcgatta ttattatccc agctatgtct ctaattctaa gctgtcttac
    14641 aatttctttt gcagcttcaa gattagtttt ataaacggtt tcatcaagtt taccagttcc
    14701 aataaactta cccgtattta catctataac cgtaagagct tctgtttttt caattattaa
    14761 ataacctcca ctctttaacc atactttttt atttaataat ttttctattt tagattctat
    14821 tctgtataaa tcaaatacat ctctattttc ctcaagacat agtttatcaa cataagtctt
    14881 atctatactt cttaaaatag acttaagttc agagtactta tcataactat tggtaattat
    14941 agattcaata tcatcattca cattatcttt tacgtattta gttgcaaaat ctaaagactt
    15001 atataataat ttcgggccta taccaagttt atattctttt aaaatgtttt catactttaa
    15061 ttttaactct tctatatctt tctttatttc atcatgagta caaccttgag cctcagttct
    15121 tattattaat cctagatttt ccttatttac tgccttagta atttttttta atctaaatcg
    15181 ttctttttca tcagtaattt tatttgatat tgtagttcta tcattggaag gtatatagac
    15241 aatatacctt ccagaaatac taatctcagt gtttaatttt gcacctttag ttcctatttc
    15301 ttctttattt atctgaacca aaatatcctg accagatttt atagtacttc ccttttttaa
    15361 ttgtaaataa gcaagtttct taaatccaat atctacaaaa caagcttcta ttcctggtat
    15421 aacttttttt acgattccac gatatatatt ggatacagtc ttcttattta aattgtcttc
    15481 aacaaaaagt tctgttaatc tttcatcttc aagtactgct gttttctgag accctattag
    15541 ggactcaata actatctttt tcacaacttc acctctcgag cttacatagg agtcactaat
    15601 tccccatctt ctaatacata taaatctctt ctcagtatat caacatctaa tgggtcaata
    15661 tcaagttcaa acatctctaa tatctttggt ataagtatat tagtatttaa attagttttt
    15721 gagcctgtag ctatagtagc agtaagagta atatgttcat cttctaaatt taaaacatca
    15781 aaggttctta tcataggtct tatatctact tcaaccatct tacctttttt attctttttt
    15841 gttatgatta tttctttatt attcataaag tcaattactt ttctttttac aaactctttc
    15901 gtcataggtc ttttcaaatc tatattaaac aaatattctc catagtctat cacagaagaa
    15961 agagaaggtg tttttctatc aatttctttt gctttgataa aatctatccc atttggaagt
    16021 tgaattgata ctttatttaa aaattcctct actgataaat catcttcttc tatttcaata
    16081 tctacatact caccttgact ttctgtccct aaagccaaag cattgccata actcatttta
    16141 gggtgaggat taaatccttg agaatgtgat aagtttattt cagctcttct aaatgctctt
    16201 tgtaacaatc tctgaaggtc aagatgtgat atatatatca tgtcgccttc ctttttaaat
    16261 tttactctta ttatcttact catattagca aagccccctt cctatatcat gtatatttat
    16321 tccacagcca ttgcagttat gtctacaatc agaagtgatt ttttcttttt tagcattttc
    16381 gtcttctctg attaagaatt ttttactaat acctacatct atatggtccc aaggaaatac
    16441 ctcttcatag tttctatttc tatgtgcata aaaatctata gaaagattat tcttttccat
    16501 agcttctttc catatatcta aattaaagtg ttctgcccat ccatcaaatt ttgcacctaa
    16561 tctaaatgca tcatatatca cttttcctat tcttctatct cctctagcta ctactgcttc
    16621 cattaaactc gtctttgaat catggtaatt gtattttata ttgttgtttc ttaatttatt
    16681 tactaaatgt ctctgcttat taataacttc ttctgtagta tcttgaccat gccattggaa
    16741 aggtgtaaat ggctttggta caaatgtaga agtactcact gtaacactaa aacttctctt
    16801 tagctttcct ccatttactt ttctataaat atcaataact ttataagcta atttagctat
    16861 accatctaaa tcttcataag tctcagttgg taaaccaatc ataaaataca acttaacact
    16921 attccacccc atctcaaaag ctctttctgt tgcattttcc aaatcttctt ctgtaacacc
    16981 tttatttata acatctctaa gtctttgagt tcctgcttct ggagcaaaag taagcccaga
    17041 ttttcttact tgttgtattt tatcagcaat ttccattgaa aaattatcta atctaaggga
    17101 tggaagcgaa atcccaatat tattagatgc atattcatct accaaataat cagtcaactc
    17161 tgataactta ctataatcac tcgtactaag tgatgataat gatatttcat catatccagt
    17221 attcttaact aatttatcta atatttcttt aagtctttta acactctttt ctcttattgg
    17281 tctgtatatc atacctgctt gacagaatct acaccctcta gtacatcctc taaataattc
    17341 tagtactatt ctatcatgaa cagtatctat ataaggtact attagttttt ctggataatc
    17401 tacagtctca acatctttta ttatcctttt agtaggattt ttagtaatcc cttccctgtt
    17461 aggagttaca ctttgaactg taccatcttc attgtacttg acatcataaa aacttggtat
    17521 gtatactcct tctatgctag atattttata taaaaaatct tctctagtcg ttttattttt
    17581 cttccattct ttatactcat ttactacttc taaattaacc tcttcacctt cacctaatat
    17641 tactacatct acaaaatctg ctaatggttc ggaattataa gcacaaggac caccaaccat
    17701 tataaatggg tcttccaaag ttctttcttc ttttaatatt ggtatattgg ctaaatctaa
    17761 tatatttaag atattagtgt atgaaagttc atattgaagt gtaaaagtga caaaatcaaa
    17821 gtttgttata ggctctcttg actctaatgc aaataatggt atactttttt ctctcatcat
    17881 attttccatg tcaactgcag gagaatacac tctctcacaa aatacatctt catccttatt
    17941 tattacatca tataatatat gactccctaa gtgactcata ccaacttcat ataaatctgg
    18001 aaagcaatgt gcatatctta tcaattcact attactagta tctttatgaa ttgaatttat
    18061 ttcatttcca aaatatctag caggcttttc tacttttttt aagatttttt tcaagtctac
    18121 tttattcatt tattaaaatt cctccaaaca agctctttat ttttattctt atttataagt
    18181 aatattatac acgataaaat attttctatc aaaaatataa ctaaaata
//
LOCUS       DAEMAE010000062        17947 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEMAE010000062 DAEMAE010000000
VERSION     DAEMAE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.063"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            229..1071
                     /locus_tag="KPH93_001677"
     CDS             229..1071
                     /locus_tag="KPH93_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1688504.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVARLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            complement(1269..3602)
                     /locus_tag="KPH93_001678"
     CDS             complement(1269..3602)
                     /locus_tag="KPH93_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1688505.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVMERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEATHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVFGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(3599..4282)
                     /locus_tag="KPH93_001679"
     CDS             complement(3599..4282)
                     /locus_tag="KPH93_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688506.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(4354..5595)
                     /locus_tag="KPH93_001680"
     CDS             complement(4354..5595)
                     /locus_tag="KPH93_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1688507.1"
                     /translation="MKIFSNKDIKMFFLILISILVMFIIVGQIVLISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKSMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDSFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQNREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            complement(5606..6289)
                     /locus_tag="KPH93_001681"
     CDS             complement(5606..6289)
                     /locus_tag="KPH93_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1688508.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFNIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            complement(6813..7076)
                     /locus_tag="KPH93_001682"
     CDS             complement(6813..7076)
                     /locus_tag="KPH93_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1688509.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWN"
     gene            complement(7249..8430)
                     /locus_tag="KPH93_001683"
     CDS             complement(7249..8430)
                     /locus_tag="KPH93_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG1688510.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            complement(8496..9044)
                     /locus_tag="KPH93_001684"
     CDS             complement(8496..9044)
                     /locus_tag="KPH93_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG1688511.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            complement(9057..9896)
                     /locus_tag="KPH93_001685"
     CDS             complement(9057..9896)
                     /locus_tag="KPH93_001685"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG1688512.1"
                     /translation="MRKIKSEQIVEQVKKICIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            complement(10093..10566)
                     /gene="ybaK"
                     /locus_tag="KPH93_001686"
     CDS             complement(10093..10566)
                     /gene="ybaK"
                     /locus_tag="KPH93_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG1688513.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            complement(10689..11387)
                     /locus_tag="KPH93_001687"
     CDS             complement(10689..11387)
                     /locus_tag="KPH93_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688514.1"
                     /translation="MEKIRLSIENINKRYDSHVIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNKVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGLRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            complement(11375..12160)
                     /locus_tag="KPH93_001688"
     CDS             complement(11375..12160)
                     /locus_tag="KPH93_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG1688515.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFAIVFPILYDNVLGAVLNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            complement(12132..13127)
                     /locus_tag="KPH93_001689"
     CDS             complement(12132..13127)
                     /locus_tag="KPH93_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1688516.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNSKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKDGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPGELGAYSEKIKITT
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            complement(13233..15167)
                     /locus_tag="KPH93_001690"
     CDS             complement(13233..15167)
                     /locus_tag="KPH93_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG1688517.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHRDKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFGINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            complement(15352..>17947)
                     /locus_tag="KPH93_001691"
     CDS             complement(15352..>17947)
                     /locus_tag="KPH93_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1688518.1"
                     /translation="NEKEKFKVLTDNILGGVKTCLKDEYMTMTYVSQGFLELTGYSCE
                     DLNKIFKNRFKDMIYKDDLDKFNCIILNQLAKQDKIEVEYRIVRKDGSIIWILERSKS
                     INDNKGSECIQGVLTDITELKKIQEELDSKRFEIQAINDSIVGGIVITEINDDFDSIY
                     LNEGYLNMIGYTRKQLKDELNNKLIKLIFKDDRTELINSINKQLKLSDTFASEQRVEK
                     RDGTIIWILLKGRKIIYDNGKSVLCCVIVDITSAKLLEQELKKSEERHEIILKQTQNI
                     IIEWDIEKDKMNISPNWYNKFKYDPITENATKNIIKSVDIYHDDMDKFISLIKLTSQG
                     SEYEECEIRLRTRDTIYAWFSIKATTLFDNNGKPYKVVGSLTDIDESKREAEKLKERA
                     EKDPLTDLYNKKASQSLIEEYITLDKKRKGALMIIDIDDFKGINDNLGHLYGDAVLSE
                     VANDLTNLFRATDIVGRIGGDEFIVFIKDISKTRDIINKAEELVKTFERSFLGKGRNY
                     KISLSVGIAKFPQDGMDYMTLFRNADMALYSAKEKGKNCYTLYDKKIDKVQYITNTRS
                     AGDSDNVEMSFKEHITNYIFDILYESSDIEVAVNLILSIVGKHFNVSRMYIFENSDDN
                     RVCKNTFEWCNEGITSEINNLKNICYKDLGNYLLNFNADGLFYCNDIENLGSDVKKVL
                     KPQGIRSILQCKILEGNEFRGFIGFDECSYNRMWTNDEVNTLTFISKTLSMFLLKMRI
                     QSKLEESLTIQKSVMDNMDIWAYVVDRNTYELLFINKKMLESAPNTHVGDLCYKAIWN
                     NQDKPCKKCPMIYLKDGDDTYKSFMRNKKFEVQANITTTTLKWFDGKDACLIFGSEVT
                     SIEAEENT"
BASE COUNT         5776 a         3171 c         1895 g         7105 t
ORIGIN      
        1 ttttatttca atacttttta acttattttg taaaaatttt aataaaacct aaagacaaaa
       61 aagaaattgg agtttatact atataagaag ctagagaatt gaaaaataca taaataacta
      121 tattatttat tgtaattttc actaatacta atatagatac tgtgacaaac tactacataa
      181 gtatattatg gctaaattat aaatataaaa tgagaggatg attttaattt gataaacata
      241 ggtgatttta ataaacttac agtaaaaaga aaaactgaat ttggatattt tttggatgga
      301 caaactaata acacaaaaga cgatatactt cttcataata gattaatcgg aaaaaatgaa
      361 ataaacatag gtgatgaagt gaatgctttc atatttaaag attctgatga cagaactgct
      421 gcaactttaa taccgccttt agctaaggtt ggggatgtag cacgtttaaa agtagttgat
      481 aatacagata ttggtacttt tatagatatg ggacttccta aagacatact agttcctttt
      541 aaagctaaga cttatccact atttagagac gaaaaatatt tattttatat atatttagac
      601 aaaagcaaaa gaattgctgc tactacagat atagattcat acttattaac agaccatact
      661 tacaatgtag gagatattgt aactggagtt gtatatggat tccaaactaa taattctgca
      721 atgatatgtg tagacaacaa atatgctggt gtaatacttc ataatgagta ctttactgaa
      781 ctaaaaactg gtgatgttct tgaaaattta catgttataa aaatatatga agatggaaaa
      841 cttggtcttt ctcctagagg aaatagaaag gatgaacttg atactttaga aaataaaatt
      901 ttaagttatt tagaaggttc tgatggatat atgagattta atgataaatc tgaccctaaa
      961 gatatttcta tattatttaa ttcaagtaag aaaaatttta aacgagctct tggagtgctt
     1021 atgaaaaaag gattaattta tcaagatgaa gaaggaactt atctaaaata gtatatttaa
     1081 aatattttaa cttaaatgct cacgttgtca tataatgttt agattcacat catctttata
     1141 gaaattttgt aacataacaa aaattttata atataaaaaa cgaagtaaaa atgaaagata
     1201 ttttctattt catatttact tcgatttttt atttaaatta ctacctctat ttaaattact
     1261 acttatattt attgtgcatt tactacctct attatgtcta attcacaaat ctttttaatt
     1321 ggttttctta ctgcaagcaa agttattatt atcatagtta ctacaatgat aaacagtaaa
     1381 tcaaaaggta tttgccagtc tatatggaat tttaatgtta ctaaggatac aaatatatat
     1441 ctatgtaata ttaatccaaa tacactcccc aataaacagc cacttacagc atatgtactt
     1501 gattctacta ttaccatttt tcttaattgc ttattggaca tgcctattgc acgcataata
     1561 ccatagtaat ttattctact tgtaacgctt atattcatac tgtttatgat attaaacata
     1621 cttataatcg caattataaa tacaaaccca tatacaaaaa ctgccatagt gtaaaatgaa
     1681 tttttagcct ctaagttacc ttgtcgctta tctcgaacct ctaaattatc atctagaaca
     1741 tttcttatat tttggattaa atcttcagga gcatcttcat caacttgagt atcaattatt
     1801 atatagtctt ttatatctgt tatactagta aaagtatttt cagatgttat aatatttcca
     1861 atatatttgt ctttaacatc aaatgaaatt gaagaaagta tacctgctac ctctatctct
     1921 ttcttagaat ttccattttt aatatatatc ttatctccaa tattaaaccc atgaccttct
     1981 tctaccaatc cgatattttc tgactctaat ttatcttcat tgatttcccc ttttactaga
     2041 tactttttgg cccagtcaaa ttgatttttt tcataagaaa ttagctgaga tgttgcctcc
     2101 ttattattgt atgtagcctc tacatccgtc atcattctac tatatacttt ttttattcct
     2161 ttgatattac ttatttcatt aatatcgtct ttagtaagaa cagacttaaa gtcagcgtca
     2221 acaatagata tatgagtagc ttctggtttt ataggtttaa gtgcctgata cataaagtct
     2281 ataaatacac taaaacctaa aaacaataca atactaatag caaatgaaga agtcataagt
     2341 ataaaagatt tttttcttga aaaggcatga tttattccca tagaaacatc aattttagaa
     2401 cttcctgcgt tagcaatttt acttgctttt ccactagtta aaatttcttt gaacattaga
     2461 ttaccacgta tagcacttag tggagacacc ccagaagctt tttttgctgg aacaatagct
     2521 gataatatta ctgttaaaaa tcctactatt attccaaaca caataccaat aaagctaacc
     2581 tttaaaattg gcatcgaacc aaataactct gtattcacat atttgagaaa tatagaggat
     2641 ataatggtta caatacatcc taataacaat cctagtggaa tagcctttac acttagaatt
     2701 aaactttcta aaacaacaaa ttttttgact tggagttttg atgctcccaa gcaacgtaat
     2761 agtccaaaaa actttattct ctccattaca cccatattaa agctattata tatcatgata
     2821 atacttgcta ttaacacaag tataaaaagt cctcctgctg ttaagtaaag tggtttagaa
     2881 taatcagagt caccttgacc tataatagaa agtaattttt catttttacc gatttgttta
     2941 tcacttaaat tgaaggtatt cttaatatca gaagtagctt ttctaatatt aaccttgtct
     3001 ttaaatgtaa tataataata atcatatgtt tctgattttt tgatagcttg tgtacctaat
     3061 attgaaagca ttaatccatg cttatctgag gctcttaagc ttgataatga tgaatattca
     3121 cctactatct tataattttt atttctacca tctgctaatt ttattttgat aaaatctcct
     3181 acttttagat taaagctttt aactgcatct acatctatta aagtctcatt ctccacaaca
     3241 ggataacgac cttttattat ttctagacct aattgttttg ctaaatcttc atctaaacct
     3301 tgtatcaaaa tctcctttga tttaaactct ccattatcaa tgacttgtcc tctaccatag
     3361 tgagctatat ctactctact acttatttct gatatagctt cttcattaag atttttaagt
     3421 actatgtgcc aatttccatc atcttttata aattgtactt tttgacattg tatagccatg
     3481 tctgctagtc caaaaatagc agtaactaaa catactgcaa tgacaataca taaaattgta
     3541 gttctatttt ttttcttatg tacctttgca tattgtggta ctagttctaa atagcttctc
     3601 attctctatt cacccctagg tctttaagca ttccatcaga aacctgtagt atcctatctg
     3661 ctgtagaagc tatatttgta ttatgagtta tcataataat agtttggtta tatttttctg
     3721 ctgtcacttt taaaagtgaa attacatcac tgctattttt actgtcaaga tttcctgttg
     3781 gctcatctgc caatataatt gatggctttg taatcaatgc tcttccaatt gcaacccttt
     3841 gttgctgacc accagataat tgatttggaa gatgactttt tctatcttta agacctaaaa
     3901 ttgtcaatac ttcatctaga taaatagtgt ctggtttttt gtaatctagt agtaaaggaa
     3961 atattatatt ttgttccaca gttaattcgg gtataagatt atatgattga aagataaaac
     4021 ctatattacg acgtcgaaaa atagtctgtt tttcttcttt cattgaacat atttctttac
     4081 catcaacaaa tatttttcct gaggttggag tatcaagacc tcctaagaga tttaatagtg
     4141 tactctttcc tgaaccagat tctccaacta cagtcacaaa ctctcctttt gaaactgtaa
     4201 aggatacatc tttcaatgcc tctacctttg tatatccatt tccatatgtt ttacataaat
     4261 tattaaccac aagaatatcc attttctctc tcacctttct aattgattaa tataataata
     4321 atctatatac cttactgcaa gatgtatttt atcttacata ttagtaagct ttaaaaaact
     4381 tatggtgaat gtacttcctt gtgatagttt actttctaca gaaatagtac cattatgttg
     4441 ttccacaata gatttaacta gtggtaatcc cagtcctaaa ccttgattat cttttgaaaa
     4501 cttacttcta taaaaccttt taaatatatg atgaatatcc tcaacatgga ttccatttcc
     4561 agtgtcttta actgaaacaa tggtggttat agtcgtctca aaccactcta tcgttatttt
     4621 atcattctct tttgtattat ctaatgcatt ctttataaga ttacttaaag cctctgacat
     4681 ccattcataa tcgcagaaca acaatgtatt ttctgaacca tttatagaaa taacctttcc
     4741 ttctttttct gccctagttt caaaactata caaaattttg acaattaaat ttttaagatt
     4801 aacattattt ttattgagaa ctattgtttt tgaatctatc tttgtaatct ttagtaaatt
     4861 cttaatcaat gtttcaatgc gttctagttc actttgactt ttttgtacaa aagcttcaac
     4921 tacatcacta ttatgacttt cttcttgtag tatctgattg tacattatta aagctgtaag
     4981 tggagttttt aattgatgcg atatatctga aattgtattt ttaagaaatt ctctattctg
     5041 tttttctttt aatatatgtg tatttaaaga tgtcgacata acatctattg aactaaataa
     5101 ttttgataag ctaccttctt catatgtatt tagtctttca tttatatttc cattcataaa
     5161 tgaatcaatt ttataactag cctcttctat cttactttgt tgcctaaaaa aatacatata
     5221 tagtatgaaa ataatcatag taaaaaatac taataaatag atagacatac tttttatata
     5281 tttactttga aacaatttta cacttggtgt aaagctaaca tctatatcaa atccatatcc
     5341 agctgtatct agtatttctt ttccttttaa aatatattta tcttcatgtg caacaaatgc
     5401 tgatggtatt ttagatttat caacacctat actatataag tatccagcta tattataatc
     5461 atgttctatc aacgtttttt tataattgtt agttatactt ataagcacta tttgccctac
     5521 tataataaac attactaaaa tagatataag aattaaaaaa aacattttta tatctttatt
     5581 agaaaatatc ttcatattaa taccctcact taattacatt ccatttataa cccattcttc
     5641 taacggtcaa tagaaactta gggttttcag gattatcttc aattttattt ctcaatcgtc
     5701 ttacatatac tgataatgta ttgtcatcta taaagctacc atttccatcc cataacttgt
     5761 ccaatatgtt ttttctagta agtacaatat ttttattttt cataaataag cacaataatt
     5821 tatattctgc tgtggtaagt tccacctcaa attcaccttt aaaaacacga ttttcaagta
     5881 atttaatagt aatattattt gagcttaatt cattcacatt acttgcaaca ttataacgtc
     5941 taagtaatgc ttttattctt gaaattaatt catttaattt aaaaggtttt gttatataat
     6001 cgtcgccacc catatcaagc cccatcacta catttacctc ttcatctgaa gcagtcaaaa
     6061 atataatggg tatattagaa acctctcgta ctttttcgca aatctgaaat ccattaccat
     6121 caggtaggga tacatctaaa agcaataagt cataattttt ttcctcatac atttgcatag
     6181 cttcttttac tgtccttgct atgtctatat taaatccact tttttgtata gagaactcta
     6241 atccatatat caagcttaaa tcatcttcaa ctaacaatat actactcatt taatcacctc
     6301 tattttaatt atttttactt ctaatacatt attacactta atttattata attataattt
     6361 tactttttca aaaactcaat atattaaaac aaatgttttt acaaaagact acacaaatca
     6421 acagtctaat gattggtatt actcctataa ctctcatcaa tatatacact atctcaagta
     6481 gctagaaatc agcttgacct gtattatttt atataaaaaa tagaaaattt tataattctt
     6541 tatctgttaa tagttaaaca gagaattgaa aataatgatt ttgtagttgg atttgacttg
     6601 aggtataaaa agacatggat atataagtag aatataaata acatatttta gattttcaca
     6661 atttgaaata gaaagaggga tgagaacata tttaagcata caagaataac tgaactacca
     6721 agtaacaaaa caggtggtat gctataaagc acaccacccc ttattctcaa caccatattt
     6781 catgttttgc atataactaa ttttgcataa aattagttcc agcgttttaa tgttggaaag
     6841 aaattgttca tcattgttct tgcttcatct tcagtcatat ccttattact ttgaagcata
     6901 tgagggatac aaacttctat catctcttcc atacttatat cagctgtaaa ctcaccatct
     6961 ttaaaacaat aactacaata atcagtactt ttacttccat ctttatttgt cccatataat
     7021 tcatcagttt gtcccattgg cattgcacag cattggcaaa atttagtttc ttccatctta
     7081 aatcactcct tatatttttc tttggctcct aaccatattc aaattatatc aagactaata
     7141 tgacattatt atgtcatatt aaaaataaaa agactaataa aatcatccca ctgtacataa
     7201 tttaattagt cttttatact tttttagttt ttaataaatt gtaaaaaatt atattctatt
     7261 tactttggtt tttatcgctg cttctttaac tgccttagca acatttttta ctacattctt
     7321 attaaataca tctggtaata tatactctgg gcatatttct tcatcactta ttgtattagc
     7381 aattgcatat gcagcagcta tcttcatttc ttcatttatc tctttagcct taacatcaag
     7441 tgcacctcta aatattccag gaaaagccag tacattattg acttgattag ggaagtctga
     7501 acgacctgtt cccattataa atgctccacc taattttgct tcttctggca ttatctctgg
     7561 agtaggattt gccattgcca atattattgg cttctcagac atactttcaa ccatttcctt
     7621 tgtaacagct cctggacctg aaacgccgat aaatacatct tttcctttta taatatcttt
     7681 taaaggtcct ttttcatttc ttatatttgt tacttttaac atttcttttt gaacatagtt
     7741 tagattttct atactagctt ccaatgctcc acttctatca cataagatta tatttttaac
     7801 attcatatta agtagtatct ttgctatggc tataccagca gctcctgctc cgtttataac
     7861 tatttctaaa tcttctattt ttttattttc aattaacttt attgagttta ttatggctgc
     7921 tgcaactatt attgctgttc catgttgatc atcatgaaaa acaggtatac ttaaagattt
     7981 ttttaatctc tcttctatct caaaacatct tggagaagat atgtcttcta aatttatacc
     8041 tccaaataca ggttccatca actctacaga tttaactatc tcatctacat cattagtctt
     8101 taaacatatt ggaaaagcgt ctacatctgc aaattgttta aataatatag ctttaccttc
     8161 cataacgggt atagatgctt cagcaccaat gttacctaat ccaagtacag cacttccatc
     8221 actaactact gctactagat tccctttaga agtgtatcta taaacatcat cttgattttc
     8281 atgtattttt aaacaaggtt ctgcaacacc tggtgtatat gctgttgata aatcatcctt
     8341 agttttaact tctaccttgc tttctacact tactttccct ttgttgattt catgcatttc
     8401 taatgccaat tttgcataat ccttagacat caattttcct ccttaaaata tacacaacta
     8461 tttttaattt tgatattcat atttatttct aaacattatt tacattcata aattgctcga
     8521 ccttgctcat atatattatt tccatagcta tctattgcta ctgtaagttt aaggtcttct
     8581 actacaatct ttcttatagc ttcggctcct aaatcttcat atgcaataat ttcacatgat
     8641 tttattgaat ttgaaatata cgctccagct cctcctattg cagcaagata aactgctccg
     8701 tatttttgca taccctctat aacttcatca cttctttttc ccttgcctat cattaatcta
     8761 agacctcttt ctaaaagagg tatagtcaaa tcatccattc tataactcgt cgttggacct
     8821 gcagcaccta taacttctcc tggctttgta ggagtaggtc ctacataata tattccttga
     8881 ccatatacgt caaatggtaa ttcttcacct ttatttatac aatctatcaa tcttttatga
     8941 gcagcatctc ttgctgtata gagtatacca ctcaaactaa ttgtatctcc acaatttaat
     9001 tttgctatat caatttcatt tacaggtgta gttattttta tcattttatt ttcctcctaa
     9061 attactacct tcttatgtct tgatgcatgg cagtttatat ttacaacaac aggtagacct
     9121 gctatatgtg taggaaaagt ctctatgttt aatcccaaag ctgttgtagt accacctaag
     9181 ccttgaggtc ctataccaag tttattaata gctgttaata gttcactttc aagttttgct
     9241 atattttcgt ccttattaaa ttcacctagt tctctaaaaa gtgccttttt agctatttga
     9301 gcacatttgt ccacagtccc acctatacca actccaatta ccataggagg acatgggtta
     9361 ggtccagctt ctgaaactgt atctattatg aattttttaa ttccttccaa tccatctgat
     9421 ggctttaaca ttttcatctt actcatattt tcgcttccaa atcccttagc tgcaaattct
     9481 attttaattt tatcaccttt taccatatca taatgaataa cagcaggtgt attatctttt
     9541 gtatttactc tattaattgg acttacaaca gattttctta agtagccttc ttcataccct
     9601 tgtcttactc cctcatttat agcatcagta agtgtgtctc cttcaattaa aacttcttgt
     9661 cctatttcaa caaaaaatac agccatacca gtatcttggc atattggtat atttttttct
     9721 ttagctattt cagcattttc aactaatata ttcaatatat tttttccaac ttcactcttt
     9781 tcgcttttag ctttctcttt tatacaactt aaaacatctt ctcctaaata taggctcgct
     9841 tcaatacaaa ttttttttac ttgctcgacg atttgttcag attttatttt tctcaagcga
     9901 acatccctcc attattttat aaaaaatatt cgtgattata cttacacttt accattgttt
     9961 ttaaaatttc tcaatatttt tataccaatt acacataagt aactgaatat ttattttaaa
    10021 attattattc attttagtaa tactacaaca cttattatta agaatataag ctctatattt
    10081 tatatgtaat tcttattttt ttattacatc tacaaattct acttttataa gtctttgtaa
    10141 atcaaatgga gatagttcta tttgatatcc tatttttcct gcactcacaa taattgtatc
    10201 taaattcttt gcactttcat ttacaaaagt tttataaagc tttttcatgc ctataggaga
    10261 acatccacct ctaatataac ctgtaagctt atttatatct tttacatgaa tcatctctac
    10321 acttttctct ttagctactt ttgcagcctt ttttaagtct aggttttcat gtactggtat
    10381 tacatataca tatatattct tactaactcc ttgagttact aatgtcttat acacttcatt
    10441 tacatctctt cctatatcat gtgccacctc aacgccatcc acatgttcac ttttcacttc
    10501 ataagacatt actttataat caatcttatt tgaatctaat attctcatag cattagtctt
    10561 aaccatacat tcacttcctt tttataaaat acgaacatta tacacagttt tgaatattct
    10621 gtactatttt aatatagtct tatagatttt aaccaaatat attttaaaac gacagtatta
    10681 ttataactct atatcaactt caaaacttca tttttttcta attccttaga attttttaat
    10741 atttttttta ttctaattgg agaatctcct aaaataaata tcctatctgc taaaaacaat
    10801 gcttcctcaa tattatgagt tacaaataac acagttcttt tttcttttct taatatattt
    10861 ctgaaattat ccattataag ctgtgtattc tttgcatcaa tagatttaaa aggttcatcc
    10921 attataatgt tttttgatgg atatataaaa gctctagcaa tatttactct ttgtctaaga
    10981 cctccactaa gcatctgagg atagaatttt ttataatcac taattccaac caattccaag
    11041 tattcatcac aaattttgtt taattgagtt ttattatagt atttatttac aactatcttt
    11101 atattttctt ctactgttaa ccagtctata agtctatcat cttgaaatat gtaactagct
    11161 tcaagcttat ttcctacttt atttaaattt tctttgatat ttagattgtt ggtgtcattt
    11221 ttaataatcc cacttattat gttcaagagt gtagtttttc cacaaccaga ttttccaagg
    11281 atacaattta cctcattcac ataaaaatca atattaaaat ctctaaatat aacatgacta
    11341 tcatatcttt tatttatatt ttcaatactc aaccttatct tttccataaa aaagctctcc
    11401 ttgctaaaaa cttttgagct atttgcaaaa tagcagaaat taaaagaaca ataattatcc
    11461 atgcaaatat tccagatgta ttaaagttaa ttttttcagt ctgtatcata gttccaatac
    11521 catatgtagg ttgactatat acctctcctg ctatagcaac tttcaaactt aaagagaatg
    11581 ttgatactag tatagatata atttgaaatt ttatggctgg taaataaatt tttaacacct
    11641 tatctataaa ccttatttta tacacattag ccatttctaa taagttcttg tctatattca
    11701 atacagctcc caaaacgtta tcatacaata taggaaaaac tatagcaaat ccaactataa
    11761 aaggggtact atccttttca aaccaaataa gtgcaagtac taccaatatc atagttggta
    11821 ttgaacgtgt caaagcattg attggtttta ataagtttct aaaaaatcta tttaaataag
    11881 acactattga aagtataata gcaaaaatca tagccagtaa aaaactaaaa aaacatctcc
    11941 ccatagagta aagtatgtct atatagaaac tagaatttag cataatctct tttatactat
    12001 taaatacttg acctactgtt ggtaaaaaaa tatcattatc aatttttaaa gcaataattt
    12061 gccataaaaa taagaggata acacaagaaa gtgttacctc cattttatct ataaattgtt
    12121 ctttattttt tttatttctc aacaaagtat attgcttcat ctggtacttt gcctcctaat
    12181 gtcttatcat caaaattagc taacttctca taataatttt tataatcttt aatcatatcc
    12241 ttaactggaa tatattttaa attggctctt tcaagagatt ttcctattat ctttgattca
    12301 gtagttattt ttattttttc agaatatgca cctaattctc caggattttt gtttgcccaa
    12361 tctatactat ttgataattg acctacaaaa gaatcaacaa aatctttatt atctcttaaa
    12421 aattcagact ttacaattaa agtagattga ggataaccgt cttttgaacc acttacttcc
    12481 ttccatgagt catttaaact ttttataatt tttatatctg gatttttaga catcaaagca
    12541 ctaagtgcag gttctggaac aatacctgtt ttaactttac cagtagccaa taatggtacc
    12601 agttcacttg ctgaatttac ataattaaat tttatatctg aataatttat atccttttgt
    12661 aacaaaagac tttttatagt tatgtctgga gtaagtcctt ttccagtaca tccaacttcc
    12721 ttaccaacta aatcagagta atcttttata ttttctgttg acaccaaata aagtgaaccc
    12781 atccctacag ttccaacaat attatatgat gaagttttat tatacgctat tgcagccatg
    12841 tttgaaggaa ctatagctat atctgcatct tttttcatca ctctagtgga aatcgcctca
    12901 ggtgtctttt caatgctata cactgtttca tatccatctt taatctctgg attttcattt
    12961 gcaagtttag ctattgccac tgctggtagt ccatctggaa cagctacttt cacttgtttg
    13021 acaactgata gctccttatt tttagagtta tcttttcctg gtgaacatcc taccaaaaca
    13081 accatagtca ccaccactaa taatattata gaaatatact ttttcatctc ttcctcccaa
    13141 agttaacgtc attaaaataa ttatatttat ttatactaat ttctgttaat ttatcgaata
    13201 atccttcttt tttaagtatt tttatttata ctttaatgtt ttttcttttt tttagttgaa
    13261 cttttttgtt gatttttatt acttctatta tttgtattag tttttttgtt tttatttgtt
    13321 cctttagata catttttttt agaattattt ctattaaaat aaggcttttc attttttggc
    13381 tttataaggc atctatgtcc aaagccaatt aagtcttctc ttcctgcttc aactaatgca
    13441 gagtatacca aatcataatt tctaggagct ctatattgta ataaagccct ttgcattgcc
    13501 ttgtcacgtt ttgatttagg aacataaact ggcttcattg taagcgggtc aatacctgta
    13561 taaaacatgg ttgtagatag agtccctgga gttggataaa aatcctgaac ctgctctggt
    13621 tggtagtgag tatctcttaa atactctgct aactctatag cactatttaa ggtagaacca
    13681 gggtgactag acattaaata aggtatcaaa tattgctttt tacctaattc ttcatttata
    13741 ccaaaaaatt tctgtctaaa tttatcataa gtttttcctg ctggtttttg catatacttt
    13801 aaaacttctt ctgatatatg ttctggagct acctttaact gaccacttac atgatgctct
    13861 atcaactctc taagaaattt attattctta tctgccatta cataatcgta tctaattcca
    13921 gagcgcacaa acaccttttt tattttaggt aattttctta cagctctaag taagtgtagg
    13981 tattcactgt ggtctgcatc taaattttta catggtgatg gagataagca ttgtctattt
    14041 ttacaagcac ctttagtaat ttgtttttta catgctggtc ttctaaaatt agctgttgga
    14101 cctccaacat catgtatata ccctttaaaa tcatctaaat ttgttattcc tattgcttca
    14161 tcaataattg atttttcact tctactttgc actgctctac cttgatgaaa tgttattgca
    14221 caaaatgaac aactaccaaa acagcctctt gaacttacta tactaaattt tacttcttct
    14281 atagctggta ttcctccaaa tttttcatac acaggatggt atgtcttctg atatggtaat
    14341 ccatatacct catctagttc ttctctacta agtggttttt ctggcttatt ttgaaccaga
    14401 tacttacttc catgaggttg aacaattata ttgcctctaa atgggtcttg ctcatcatat
    14461 tgaattttaa aagcttctac atacttcatt ttatcttcac aaatttcttt atatgatgga
    14521 attaatatat atttatcata tatttcttcc aatgtatctg aaatatagca tgtcccatca
    14581 atatgtctaa tatattttgg gtcatatcca tcattaagtg cattggctac ttctacaatc
    14641 tgcttttcac tcataccata aactaataaa tctgctccac tatctattaa catagacttt
    14701 ctaaccttat cactccaata atcatagtgt gcaaatcttc ttaaactcgc ttcaattcca
    14761 cctattacta ttgcaacatc actgtatgct tctcttattt tattgcaata tactatagta
    14821 gctctatctg gtctataacc cattttccca cctggagaat acatatcctt gtctctatgc
    14881 tttttactta cagaataatg attgaccatt gaatccatat ttcctgcatt aactagaaat
    14941 gcaagtctag gttttcctag cttcataaaa tcatctgtag ttctccagtc tggttgagct
    15001 attattccaa ctttataccc agcattttct aaaactctag atattattgc tgttccaaaa
    15061 ctatgatggt ctacataagc atctcctgta actagaacaa aatctaattc ttcccatcct
    15121 ctatctatca tatcttgttt gcttattggt aaaaacttat tttccatatt atcacctgct
    15181 caatatatat ttaacaaata tttttttata caaaagtatc ttttaatttt gttcttaacg
    15241 cttaattata tcataaaaaa tacttaaaac cacaacttaa atgatttata tagacatttt
    15301 aagtctaata aattcaaatt gcgattttaa gtatatatat aacacaatat tttaagtatt
    15361 ttcttcagct tcaatactag ttacttcact tccaaatatt aaacatgcat ctttcccatc
    15421 aaaccatttc aaagtagttg tagttatatt tgcctgtacc tcaaactttt tattgcgcat
    15481 aaacgattta taagtatcat caccatcttt taaatatatc atagggcatt ttttacaagg
    15541 tttatcttga ttattccata tcgctttata acataaatca cctacatgag tgtttggtgc
    15601 tgactcaagc atttttttat tgataaataa taattcatag gtattcctgt caacaacata
    15661 tgcccatata tccatattat ccatcacaga cttttgaatt gttaaagact cttctagctt
    15721 actctgaata cgcattttta ataaaaacat actgagcgtt ttagatataa atgtcaaagt
    15781 gttcacttca tcattagtcc acatacgatt atatgaacat tcatcaaatc ctatgaaccc
    15841 tctaaattca ttgccctcta aaattttaca ttgtaatatt gaacgtatgc cttgtggctt
    15901 aagtactttt ttcacatcac tgcctaaatt ttcaatatca ttacaataaa ataacccatc
    15961 tgcattaaaa tttaaaagat agttgcctaa atccttgtaa caaatatttt taagattatt
    16021 aatttccgag gttatacctt cgttacacca ttcaaatgta tttttacata ctcgattatc
    16081 atctgaattt tcaaatatat acatgcgact cacattaaag tgtttaccta caatacttag
    16141 aattaaattt actgccactt ctatatcact tgattcatat agtatatcaa atatataatt
    16201 tgtgatatgt tctttaaaag acatctctac attatcacta tctcctgcac ttcttgtatt
    16261 agttatatat tgtactttat caattttttt atcatacaat gtataacaat tcttaccttt
    16321 ctctttggct gagtaaagag ccatatccgc atttctaaat agtgtcatat agtccatacc
    16381 atcttgtgga aacttagcaa ttcctacact taatgatatt ttataatttc gtcctttacc
    16441 taaaaaagac ctttcaaatg tttttaccaa ttcttctgct ttatttataa tatctcttgt
    16501 tttagaaata tcttttataa atacaataaa ctcatctcca ccaattctgc ctactatatc
    16561 ggttgctcta aatagatttg ttaggtcatt tgcaacttca cttaatacag catccccata
    16621 taaatgccct aaattatcat ttattccttt aaagtcatca atatcaataa tcattaaagc
    16681 accttttctt tttttatcca atgttatgta ttcttctata agtgattgtg atgctttttt
    16741 attatacaaa tctgtaagag ggtctttttc tgccctttct ttcaacttct cagcttctcg
    16801 ttttgattca tcaatatcag tcaaacttcc tactacttta taaggtttac cattattatc
    16861 aaataaagtt gttgccttta tactaaacca agcataaata gtatctctgg tcctaagtct
    16921 aatttcacac tcttcatatt cactaccttg tgaagtaagt ttaattaatg aaataaactt
    16981 atccatatca tcatgatata tatctactga tttaattata ttttttgttg cattttcagt
    17041 tatagggtca tatttaaact tattatacca attaggtgat atattcattt tatctttttc
    17101 tatatcccat tctattataa tattttgcgt ctgctttaga atgatttcat gacgttcttc
    17161 actcttttta agttcttgct ctaataattt agcagaagta atatctacaa taacacaaca
    17221 aagtacagat tttccattat cataaattat ctttcttcct tttaataaaa tccatataat
    17281 agtaccatct cttttttcaa ctctttgttc agatgcaaat gtatcactta atttgagttg
    17341 tttgtttata ctattaatta gttctgttct atcatcttta aaaataagtt tgattaattt
    17401 attatttagc tcatctttca gttgtttgcg tgtatatcca atcatattta aataaccttc
    17461 atttaaatat atactatcaa aatcatcatt tatctcagtt atcactattc caccaacaat
    17521 actatcattg atagcctgta tttcaaatct ttttgaatct aactcttctt gtattttctt
    17581 tagttctgtt atatcagtta aaacaccctg aatacactca cttcctttat tatcatttat
    17641 agacttactt ctctccaaaa tccaaattat acttccatct tttcttacaa ttctatattc
    17701 aacttcaatt ttatcttgtt ttgctaattg atttagaata atacaattaa acttgtctaa
    17761 atcatcttta taaatcatat ctttaaatct atttttaaat attttattta aatcttcaca
    17821 agagtaacct gtaagctcta aaaacccctg acttacatat gtcatagtca tatattcatc
    17881 ttttaaacag gttttaactc ctcctagtat attatcagtg agtactttaa atttttcttt
    17941 ttcattt
//
LOCUS       DAEMAE010000063        17847 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEMAE010000063 DAEMAE010000000
VERSION     DAEMAE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.064"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..150)
                     /locus_tag="KPH93_001692"
     CDS             complement(<1..150)
                     /locus_tag="KPH93_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688519.1"
                     /translation="MLYDITSINGAYKFLSEYLEVDRDYIDLYIRRNPSKYKFKKFIE
                     EIEFIK"
     gene            complement(522..1034)
                     /locus_tag="KPH93_001693"
     CDS             complement(522..1034)
                     /locus_tag="KPH93_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1688520.1"
                     /translation="MWIYHKRWKTKRFHKIIIEWSGVMSDLKSESFKKAENKLYSYNR
                     VKTEIKNLNLEIEEKENLYMGCCSIGYGEKSGTTYNISNLVESEVLKKEKEVEKIKRY
                     IKVKEIFIEKIDNAILLLNEDEIALIKHRYFSNKKNSWNYVANKIGFSTARCKQMRIE
                     IINKLKDLLS"
     gene            complement(1220..1513)
                     /locus_tag="KPH93_001694"
     CDS             complement(1220..1513)
                     /locus_tag="KPH93_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688521.1"
                     /translation="MTLDLNIKELYELGFSEEFIALAKAHLLDDFIFNDVPKLSYTNV
                     GKMYDIKKYEIFIVVNETLIYILQTQYKEEFLPVEEQILSFGTVKKMLEYVCK"
     gene            complement(1527..1784)
                     /locus_tag="KPH93_001695"
     CDS             complement(1527..1784)
                     /locus_tag="KPH93_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688522.1"
                     /translation="MKLKKLISKLSRLEDVEIVFHINVKNIPIISSIKLSSIIMNHDE
                     VEIYGLNNFKLYIPVDEIIDIVEYPESLILYSLSFQVIFRW"
     gene            complement(1774..2403)
                     /locus_tag="KPH93_001696"
     CDS             complement(1774..2403)
                     /locus_tag="KPH93_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1688523.1"
                     /translation="MYEVELMELIDKSKDGNESAFESLLKIFDKDIQINANKYVNLLQ
                     GGFDYNDLIQVARIALWKALSNYSHTKGKLHPKAYFKKAIKLSMLSLLNSGNRKKHRT
                     LNKSISLNNFISKDEQIELITLIDNNEDDIESQVISKEITRELKNKVKKDIVYQTDMQ
                     RKVISLRVDGYEGREIERILGISLKSINCHLYRARKIIKQNLGSVNYEA"
     gene            complement(2418..3752)
                     /locus_tag="KPH93_001697"
     CDS             complement(2418..3752)
                     /locus_tag="KPH93_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBG1688524.1"
                     /translation="MSSVNDLVKSTEAEQSVLGSILLDATAIREIEPILTEDMFYHEE
                     HKIIFDSMKKIYKRNEPIDLITLTEEVKHKGHLNRVGGISYITSLSNVVPSLSNALYH
                     ANIVLDKYKNRNIIDKFNKFKLGEIDDNTLIYQLEQDINKIDKKLIKKDTSISAITTR
                     AFDYISDGVEEGIEIGIEVLDETIGGLYQGELTTIAAKSGGGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNISSLTGIPIVKMKNRDLNEKEMIDYINALGILNQDEILD
                     INDTVNTISGIKREIKRLKPDLVIVDYLQILDYEGKEVGEAKFAELSREMKKITLDFN
                     IPVIQLAQLNDEMRDERPKGDRPMRSSKQIYMDSNNVVYLHRPTEKEALEYCKLIGIE
                     DIEALRKREKSLGVKLTEIIVAKNRDGEARYTPYWYVGCRLTFKKCTMAKII"
     gene            complement(3767..4531)
                     /locus_tag="KPH93_001698"
     CDS             complement(3767..4531)
                     /locus_tag="KPH93_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688525.1"
                     /translation="MGELGWIKLHRALKDNPTWKACKAERKVIMITMLLLASHKETVI
                     TLNTGKKVKILPGQFITSRSSLAEKCGKDVSEQMVRSALQFFKKTNFSTNESTNGYTL
                     VTIINWGLYQDVESKQPTSQPVVNQGSTTIKNVKNVKNKKLYVETSNEYRLAEFLFKH
                     IRKNNPNAKTPNLQSWAKDFDYILRLDKRNLEEVQELIRFCQNDSFWCTNILSASKFR
                     KQYDQLYLKYKNEKNPLNKKAKEDGVYNPYDTDLMI"
     gene            complement(4555..4785)
                     /locus_tag="KPH93_001699"
     CDS             complement(4555..4785)
                     /locus_tag="KPH93_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688526.1"
                     /translation="MEVNENMQTKVVNLKTKKSRKIMTVKEFSKEYEIGINNAYEMTH
                     IKGFPAFTIGRKICILRDKVDEWFLDNIGNKF"
     gene            complement(4797..5234)
                     /locus_tag="KPH93_001700"
     CDS             complement(4797..5234)
                     /locus_tag="KPH93_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688527.1"
                     /translation="MLQQCQNIYQISRENARLTQEKASELLGISVESIRAYENDKRIP
                     NNTVVAKMVSIYNNKLLGYEHVRRNTEAGIMFLPELKIKNLSSIALKLHKEIKDYLKK
                     EDDFIDIVEDDVIDEEEEEVWNDVMNELDGIYEAIFALKFAKK"
     gene            complement(5262..5480)
                     /locus_tag="KPH93_001701"
     CDS             complement(5262..5480)
                     /locus_tag="KPH93_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688528.1"
                     /translation="MRSKLKQLRIKKNLTHKVMAKELGISRSYYTSIEMGNKTPSLKT
                     SMKIKSFLKYYKDDIFLDDKVSKRNNLD"
     gene            5645..5992
                     /locus_tag="KPH93_001702"
     CDS             5645..5992
                     /locus_tag="KPH93_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688529.1"
                     /translation="MSKFYERLAQLRKEHNLTQMDLAKIINKQRSTIAGYETDRKQPD
                     FDTLCTLANYFEVSIDYLLGFTEDKKTYDSTLEDLEIDDDVKKITDIIFKLDEEDREA
                     VFKMLNALTKKYK"
     gene            complement(6039..7199)
                     /locus_tag="KPH93_001703"
     CDS             complement(6039..7199)
                     /locus_tag="KPH93_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG1688530.1"
                     /translation="MDFSDKIKQFSKRIEVIKENLTTEEATKTALIMPFFQILEYDVF
                     NPLEFIPEYVADVGVKRGEKVDYAILDDGKPIILIEAKNIKDKLTKHDAQLFRYFTAT
                     EARFAILTNGIIYKFFTDLEEKNKMDEKPFMTINLLDIDENKISHLKKFAKTTFDVDV
                     VFNIASESKYTNLIKEQLNEQLESPSDEFVRFIINDFYNGVKTQNVVEKFRPIVKRSI
                     LQFATDFMNEKLKSLLESNDDEDEKEKKKIIDKKAKEETERKNKEKTEKEKMRKVEEC
                     QKEETKIEVVKEDQQQISAEEKEAFDIVQDILGDLSCKEDITYKKEKEWLNILYKADS
                     NLWICNIKLTSSDKLISLPNQNNEPVEYRMDEVIDIRELKDELIFVLSKYTN"
     gene            7953..8174
                     /locus_tag="KPH93_001704"
     CDS             7953..8174
                     /locus_tag="KPH93_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688531.1"
                     /translation="MLKQARKNKNLTQKQLSKIANISQSYISRLEQDIFINSPTIRQI
                     ISLSKALDISAYKLSNYFINKENAYNKKR"
     gene            8222..9400
                     /locus_tag="KPH93_001705"
     CDS             8222..9400
                     /locus_tag="KPH93_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1688532.1"
                     /translation="MATKRANGEGSIVKNIRNGVQIGWRASISIGRDEKGKLIRKQFT
                     GKTQKEVKEKLDIYRTKMLLGSIVSADKITFEDWFYTWLFDYRAKDLKPKSFERYEGI
                     YRNYIKDTELGKIKLIDLRATHIQRYYNKLMDVYNKPASTIITLNTRLKPCLTEAEKQ
                     GYIQKNYCKMVTLPSDNKIKEVQILSITDQKKFIDSIDGDKFEILFLLALSTGLRLGE
                     LLGLKWSDISFDDESLTVNRTLQRVTEIKKDGTREKKIIEQYPKTKNSIRTVPIPRNI
                     LVKLKRHKIQQTEQRLLLGDAYINNNYVICNDTGLALNNNRPGKILDSLLKKLNIPKI
                     KFHALRHTYATRLFEAGVPPKTVQTLMGHYDISITMDIYTHVMHNTKQDAVDKINDIF
                     "
     gene            complement(9544..9893)
                     /gene="ssrA"
                     /locus_tag="KPH93_001706"
     tmRNA           complement(9544..9893)
                     /gene="ssrA"
                     /locus_tag="KPH93_001706"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10051..10506)
                     /gene="smpB"
                     /locus_tag="KPH93_001707"
     CDS             complement(10051..10506)
                     /gene="smpB"
                     /locus_tag="KPH93_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG1688533.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            11084..11911
                     /locus_tag="KPH93_001708"
     CDS             11084..11911
                     /locus_tag="KPH93_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG1688534.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGTMPTNDNMNNKVSKQK"
     gene            12913..13377
                     /locus_tag="KPH93_001709"
     CDS             12913..13377
                     /locus_tag="KPH93_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1688535.1"
                     /translation="MNLNKVKFTEEHAIEVINWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGIKPEYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRIWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(13644..14288)
                     /locus_tag="KPH93_001710"
     CDS             complement(13644..14288)
                     /locus_tag="KPH93_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688536.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGMVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQFIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYITGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(14218..15507)
                     /locus_tag="KPH93_001711"
     CDS             complement(14218..15507)
                     /locus_tag="KPH93_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688537.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQENTYTT
                     YPTGVAKYIKLDKNKTKTIEKYIEEITSKSISEINLIWKTGESIKIDSSKVSFERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYNEAMQNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWSKFSKLFSSNKRKLQIKHFN"
     gene            complement(15504..16376)
                     /locus_tag="KPH93_001712"
     CDS             complement(15504..16376)
                     /locus_tag="KPH93_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688538.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKATLRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            complement(16905..17396)
                     /locus_tag="KPH93_001713"
     CDS             complement(16905..17396)
                     /locus_tag="KPH93_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG1688539.1"
                     /translation="MGMIGCYTKISEENVFKLQQTKENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT         6083 a         2724 c         2008 g         7032 t
ORIGIN      
        1 ttttataaac tctatttctt ctataaattt tttaaattta tattttgatg gattccttct
       61 tatatacaag tctatataat ctctatcaac ctctaaatat tcacttaaaa atttatatgc
      121 tccatttata ctagttatat catacaacat gataaaacat acctcctttt atctaataat
      181 accaatcatt ataaagaatg tatatattgt actaaaactt caaagaagga ataaaaaaaa
      241 gactagattt ataatctagc ctaattagag tatgattaac aacttctact ctacaactta
      301 atatacttat gctaatattc tattcatagt taactactta accctatctt cattatataa
      361 caagttaagt ataaaaagag tatacattta gtataataat cgtatagttt tagtataatt
      421 tttggtcata actcaaacac tttttttaca cttttagtat aatcactaca taaaacccaa
      481 cttaataggt ataatggtaa ttttgtcact agccaatgta attaactcaa taaatctttt
      541 aacttattta taatctctat acgcatttgt ttacatcttg cagtagaaaa acctatttta
      601 ttagctacat agttccatga atttttttta ttactaaagt acctatgctt aattaatgca
      661 atttcatctt catttagtaa taatattgca ttatcaattt tttctataaa aatctctttc
      721 acttttatat acctctttat tttttcaact tctttttctt tttttagaac ttcactttca
      781 accaaattag aaatattata agtagttcca gacttttctc catatcctat agagcaacaa
      841 cccatatata aattttcttt ttcttctatt tctaagttca aattttttat ttctgttttt
      901 actctgttat aactatataa tttattctct gcctttttaa acgactcact ttttaaatca
      961 ctcataacac cactccattc aattattatt ttgtgaaacc ttttagtttt ccatctttta
     1021 tgataaatcc acattttaag actttaaaaa tactatatgc ttcactcaat gttatctctt
     1081 ttatatctct aatcttaata tctctcataa taaacttccc cttataattg atttttttaa
     1141 gaatatagga tatactatac ttgtcgaggg tatagtgtat tccatacact taaggtgttt
     1201 tcttaagaaa acatcttttt tatttgcaaa catattctaa catcttcttt acagttccaa
     1261 aggataaaat ttgttcttct acaggcaaaa actcttcttt atattgtgtt tgtaaaatat
     1321 atattaatgt ttcgttcaca acaataaaaa tctcatattt ttttatatca tacattttgc
     1381 ctacatttgt ataagacagt tttggtacat cattaaagat aaaatcatct aataaatgag
     1441 cttttgctaa agctataaat tcttctgaaa atcctagttc atataattct tttatattta
     1501 aatctaaagt catatttaca ccccttttac catctgaata ttacttgaaa acttaaacta
     1561 tacaatatta atgactctgg atattcaact atatctatta tttcatctac tgggatatat
     1621 aatttaaaat tattcaatcc atatatctct acttcatcat gattcataat aatactactc
     1681 aattttatgc tacttattat aggtatgttt ttcacattta tatgaaaaac tatttcaaca
     1741 tcttcaagtc tgctcaattt tgatattaac tttttaagct tcataattaa cacttcctag
     1801 attttgtttt attattttcc tagctctgta tagatgacaa tttatactct taagagatat
     1861 tcctaagatt ctttctattt cacgcccttc atatccatct acacgtaaac taatcacctt
     1921 tctttgcata tcggtttgat atacaatatc ttttttcact ttatttttaa gttctctcgt
     1981 tatctcttta gatataactt gtgattctat atcatcctca ttattgtcta taagtgttat
     2041 aagctcaatt tgttcatctt tactaataaa attgtttaaa gatatacttt tattgagagt
     2101 tctatgtttt tttctatttc cactatttaa taatgataac atggataatt ttatagcttt
     2161 tttaaaatat gctttaggat gcaatttacc tttagtgtga ctatagttac ttaacgcttt
     2221 ccatagtgct attctcgcga cctgaattaa atcgttataa tcaaaaccgc cttgtaataa
     2281 atttacatac ttatttgcat ttatttgtat atctttatca aatattttta ataatgactc
     2341 aaaagctgat tcattcccat ctttactttt gtctattaac tccattagtt ctacttcata
     2401 cattgatatt tacctcctta tattattttt gccatagtac attttttaaa agttaatcta
     2461 catccaacat accaatatgg agtatatcta gcctctccat ctctattttt agctactatt
     2521 atttcagtta actttacacc taaacttttt tccctttttc taagtgcttc tatgtcttca
     2581 ataccaatta atttacaata ctctagagct tctttttctg taggtctatg cagataaaca
     2641 acattgttac tatccatata aatttgctta ctactacgca taggtcggtc accctttggt
     2701 ctttcgtctc tcatttcatc attcaattgt gctaattgaa taactggtat gttaaaatct
     2761 aacgttatct ttttcatttc tcttgataac tcagcaaact tagcctctcc tacctcttta
     2821 ccttcataat ctaaaatttg taagtaatca actataacta aatctggttt taatcttttt
     2881 atttctctct taattccaga tatggtattt acagtatcat ttatatccaa aatttcgtct
     2941 tggtttagaa tacctagagc atttatataa tcaatcattt ctttttcatt taagtctctg
     3001 ttcttcattt ttacaattgg tattccagtt agtgaggata tatttctaaa taaaatttgt
     3061 atatcactca tttctccact tataaataaa acttttttcc cttgaaaaat aacgtttctt
     3121 agtatctgta gtgcaagagc tgtttttcct cctccactct tagctgctat agtagttaat
     3181 tctccttggt atagtccacc tatagtttca tctaaaactt ctattcctat ttctatacct
     3241 tcttctactc catcagatat atagtcgaac gccctggttg ttatagctga tatactagta
     3301 tcttttttaa tcagtttttt atctatctta tttatgtctt gctctaattg atatatcaat
     3361 gtattatcat ctatttcacc aagtttaaat ttattaaatt tgtctattat atttctattt
     3421 ttatatttgt ctaacacaat atttgcatga tataaagcat ttgatagact tgggactaca
     3481 tttgataaac tagttatata acttatacct ccaacacgat ttaagtgacc tttatgtttt
     3541 acctcttctg tcaatgtaat taaatcaata ggctcatttc ttttatatat ctttttcatt
     3601 gaatcaaata taattttatg ttcctcatgg taaaacatat cttcggttaa aattggttct
     3661 atttctcgta ttgctgttgc atctaacagt atacttccta aaacagattg ctctgcttct
     3721 gtacttttaa ctaaatcatt tacactactc atatcttact cctctcctaa atcattaagt
     3781 ctgtatcata tggattatat actccatctt ctttagcttt tttatttaat ggattttttt
     3841 cattcttata ttttaaatat aactggtcat actgctttct gaacttagaa gcacttaaaa
     3901 tatttgtaca ccagaaacta tcgttctgac aaaatctaat taactcttga acctcttcta
     3961 aatttctttt atccaaccta agaatataat caaaatcttt tgcccaactt tgtaaattag
     4021 gtgttttggc attaggatta ttttttctta tatgtttaaa taaaaactct gctagtcgat
     4081 actcgttaga agtctcgaca tatagttttt tattctttac attctttaca ttcttgattg
     4141 tggttgaccc ctggttgact actggttgac tagttggttg ctttgactcc acatcttgat
     4201 acaaccccca gtttataata gttacaagag tatagccatt ggttgattcg ttggttgaaa
     4261 aattagtctt tttaaaaaac tgtaatgcac ttctaaccat ttgttcagaa acatctttcc
     4321 cacatttttc tgctaaacta ctcctagaag taataaattg acctggtaat attttaactt
     4381 ttttccctgt gtttaaagtt ataaccgttt ctttatgact cgctaataaa agcatagtta
     4441 tcattataac ttttctctca gctttacaag ctttccatgt aggattgtct tttaatgctc
     4501 tgtgtagttt tatccaacct agctctccca ctttgtcacc tacttcttaa cattttagaa
     4561 tttgttgcca atattgtcta aaaaccactc atcaacttta tctcttaaaa tacatatttt
     4621 tctacctatt gtaaacgcag gaaatccttt tatatgagtc atttcataag cattatttat
     4681 gcctatttca tattccttag aaaactcttt tacagtcatt atttttctag attttttagt
     4741 tttaagattt acaacttttg tttgcatatt ttcattaact tccataaaaa tacctcctat
     4801 ttcttagcaa attttaatgc aaatatagct tcatatatac catctaattc gttcattaca
     4861 tcattccaaa cttcttcttc ctcttcgtca attacatcat cttcaactat atctatgaag
     4921 tcatcttctt tttttaaata atcttttatt tctttatgta actttaaagc tatacttgaa
     4981 aggtttttta tttttaattc tggtaagaac attatccccg cctctgtgtt tcttctaaca
     5041 tgttcatatc ctaatagttt attgttatat atagaaacca ttttagctac tactgtatta
     5101 tttgggattc ttttatcatt ttcataagct ctaatacttt ctactgatat acccaataac
     5161 tctgatgctt tttcttgtgt caatctagca ttttccctac taatttgata tatattttgg
     5221 cattgttgta acatttactt tcctccaatt ttgatataga attaatcaag gttgtttctt
     5281 ttagaaactt tatcatctaa aaaaatatca tccttataat atttcaaaaa tgattttatt
     5341 ttcatacttg tttttagaga gggcgtttta tttcccattt ctattgaagt ataataagaa
     5401 cgtgatattc caagctcttt agccataact ttatgtgtta agttcttttt tattcttaat
     5461 tgctttaact tgcttctcat aatatccctc cttttaatgt ttcttttgga aaccttatat
     5521 tttatatatt agtttctttt agcaactttg tcaagttttt ttagagaaaa agtttttttt
     5581 agaaacatgt agttacttat agaaaccatt ttggatataa tcttacttat ggagagtgat
     5641 ttaattgagt aaattttatg aacgactagc acagttaaga aaagaacata atttgactca
     5701 aatggattta gctaaaatta ttaataagca aaggtcaact atagcaggct atgagacaga
     5761 tagaaaacaa cctgattttg atactctttg taccttagca aattactttg aagttagcat
     5821 tgattatctt ttaggattta cagaagataa aaaaacatat gatagtactt tagaagattt
     5881 agaaattgat gatgatgtaa aaaaaataac tgatataata tttaaacttg atgaagaaga
     5941 tagagaagca gtttttaaaa tgctcaatgc tctaacaaag aaatataaat aaaaaataca
     6001 ttgactgaat tagtcaatgt attttttatt tatatttctt aattggtata ctttgatagt
     6061 acaaatatta actcatcttt caattctctt atatctatga cttcatccat acgatactct
     6121 acaggctcat tattttgatt tggtaaagag attaacttat cagaagaagt aagctttatg
     6181 ttacatatcc ataaattgga atcagcttta tataatatat ttaaccactc tttttctttc
     6241 ttataggtta tatcttcttt gcatgataaa tctcctaaaa tatcttgtac tatatcaaaa
     6301 gcttcttttt cctcagcact tatttgttgc tggtcttcct taacaacctc tatttttgtt
     6361 tcttcttttt ggcattcttc aacttttctc attttttctt tttcagtctt ctctttattt
     6421 tttctttcag tttcctcttt agctttttta tcaattatct tctttttctc tttttcgtct
     6481 tcatcatcat tactttctaa caatgacttt aacttttcat tcataaaatc agtcgcaaac
     6541 tgtagaatag acctttttac aataggtctg aatttttcaa caacattctg cgttttcaca
     6601 ccattataaa aatcgtttat tataaatcgt acaaattcat cagatggact ctcaagttgt
     6661 tcatttagtt gctcttttat cagattagta tactttgatt cagatgctat gttaaaaact
     6721 acatctacat caaaagttgt ttttgcgaat ttctttaagt gagatatttt attttcatct
     6781 atatctaata aatttatggt cataaatggt ttttcatcca ttttattttt ttcttccaaa
     6841 tctgtaaaaa acttataaat tattccattt gtaagtattg cgaaccttgc ctctgtagct
     6901 gtaaaatatc tgaacaattg tgcatcatgt ttagtaagtt tgtcctttat attcttagcc
     6961 tctattagta tgattggctt accatcatcc aatatcgcat agtctacttt ttcacctctt
     7021 ttaacaccaa catctgctac atattctggt ataaattcta atgggttaaa tacatcatat
     7081 tctaatattt ggaaaaaagg cattattaat gctgtttttg ttgcttcttc tgtagttaaa
     7141 ttttccttta tcacctctat tcttttacta aactgtttta ttttatctga aaaatccaca
     7201 taatcgcctc cccctaaaat gattggattt atagtttgtt atttttatta tacaataatt
     7261 tcgttttaaa taagaaaaat atgtcaatat atagttctga atcgttcgac aaaataaaat
     7321 acatttttct tttgctattt ctaatttcaa agtatactct attagaatct gacgctccct
     7381 tttaaagggg attgtgaaat aatctttatt tccaatttaa ttatgtgttc atcaataggt
     7441 cttcaaccct gcttagaggt tttacctttt aaaatgtaaa actcattttt atcaacatac
     7501 acatttttta aatttgtttc tatctggtct gaaaattgtt gtttaacacc taaaaaataa
     7561 ttagatttat agtttatcct tttgaattgt ttgacaaaat aaaatacatt gacaatgtgc
     7621 caatgtattt ttcataattt attaaagcta ataacatgaa tctatctcat ttattctttt
     7681 tttctgcgac tttaattaca aattttataa aataatcata atcttcttta ttcttttttt
     7741 ttaattcatt tatagaaatt gcttttttta tcttattttt gcacataaaa tcacccctat
     7801 tttgattaaa aaattaaaat ttctactttt aaataatctt atgtacatga gttatttata
     7861 cttatatatt accatatttt accatagcag aaatattcgt acgacgaata ttgtgactta
     7921 cttcgacata taataatatt gggtgacatc taatgctaaa acaagctaga aaaaataaaa
     7981 acttaactca aaaacaatta agtaaaattg ctaatattag tcaaagttat atttcacgct
     8041 tagaacaaga tatttttata aatagcccta ctatacgaca aattatatca ttatcaaaag
     8101 cattagatat tagtgcttat aaattatcaa attattttat caacaaagaa aatgcgtata
     8161 ataaaaaaag ataattatgt agtataataa atataattaa tactgtataa gaggtgacaa
     8221 agtggcaact aaaagagcta atggcgaggg gtctattgtt aaaaatataa gaaatggtgt
     8281 tcagatagga tggcgtgctt ctataagtat tggtcgtgat gaaaaaggca aacttataag
     8341 aaaacaattt actggaaaaa cacaaaaaga ggtaaaagaa aaattagata tttataggac
     8401 aaagatgctc ttaggctcta tagtctcagc tgataaaata acctttgagg attggtttta
     8461 tacttggtta tttgattata gggcaaaaga tttaaaacct aaatcttttg aaagatatga
     8521 gggtatttat agaaattaca taaaagatac tgaacttggg aaaataaaat taatagattt
     8581 aagagctaca catatacaaa gatattacaa taaacttatg gatgtatata acaaacctgc
     8641 atcaacaatt ataactctaa atacaagatt aaagccttgc ctaacagaag cagaaaaaca
     8701 aggttatatt caaaaaaatt attgtaaaat ggtaacacta ccatctgata acaagataaa
     8761 agaggtacaa atattaagta ttactgacca aaaaaaattt attgatagca ttgatggaga
     8821 taagtttgaa atactttttc tccttgcttt gagtacaggt ttaagactag gagaattact
     8881 tggtttgaaa tggagtgata ttagttttga tgatgagtct ttaactgtta acaggacttt
     8941 acaacgagtt actgagataa agaaagatgg aactagagaa aaaaagatta ttgagcagta
     9001 tccaaagact aaaaattcta taagaacagt tcccattcca agaaatattt tagttaaatt
     9061 gaaaagacat aaaatacaac aaacagaaca aagattattg ttaggtgatg cttatataaa
     9121 taataattat gtaatttgta atgatacagg tcttgcttta aacaataata gacctggtaa
     9181 aattttagat tctttgctaa aaaaattaaa tatacctaaa attaaatttc atgctcttag
     9241 acatacatat gcaacacgat tattcgaggc tggtgtccct cctaagactg ttcagacgtt
     9301 aatgggtcat tatgatataa gtataactat ggatatatat acacacgtta tgcacaatac
     9361 caagcaagac gctgttgata aaataaatga tattttttga tagaaatagg gtatattaaa
     9421 tcttaccatt tttaatgtaa atccatggtt gtgtgctgat tgtgtgcttt ccaacaaaat
     9481 aaaaaggatt gcttagccta aaactaagta atcccaatca ttcaattgtt tcattttcaa
     9541 aattggtgga gacgagggga gtcgaacccc tgtccgcaaa tcttttcctt gctgcatcta
     9601 catgcttagc caacttcact aatatctcac ctcacatagc gccaagtggc aggctctatg
     9661 cttagccagt cccaattaat ctactttaag tcgagacacc cctaaagtag ttccctgcat
     9721 aatgacgtcc agtcttagtc ggcaggagga ctaagaggga cgagcagcaa ctaggctgct
     9781 atcgcgaaat tatcgtctgc gtttatttta aagttcccca gtttttacgt gacccagagt
     9841 aacacgacat gcagcctcaa gttccaaacc cacgtcgaaa cctttacgcc cccttgagta
     9901 gtttatagtc cagtagactt ttcgcaaact ttatcaccat ttttattaaa tttttaatgt
     9961 atatataata taatactttt aagaataaaa atcaatgtta tgttctagat aataaagaat
    10021 tttattctaa aaaatacagt tttatctagt ttaatactta cctgtcattt ctctttctat
    10081 acgtctttgt gcatctttct tagccatatc ttgacgttta tcatatagtt ttttaccttt
    10141 tgctaaggca atctctactt taactttacc gttttttaga tacaaactaa gtggtataag
    10201 tgaatacccc tttatagtag ttgctccaat aagctttctt atttcatgct tatgaagtaa
    10261 tagttttcta actcttaaag aatcaacatt gaatatattt ccttgctcat atggacttat
    10321 atgaacttgt tttaaaaata cttcgctatt atctacagat gcaaatccat cacttaggtt
    10381 aactttgcct tgtcttattg attttacttc tgttcctttt aattcaattc cacattcata
    10441 agtttcttct ataaaatatt catgtcttgc tcttttgttt gttgctaata tttttttacc
    10501 agccatataa atctcacctt ctttgctaga aatttcttcc ataaatgtta gaaatttact
    10561 aaatatttaa tgttgccaaa ttatttgact gacttgtatg ataacatatt taggcatttt
    10621 agtcaatatt tcactggtat attaagttag ttaggctata tataattaag taatataaat
    10681 gtatgaaaat agctaaaaaa ctacttcgaa tatacttaat actaaactaa ctctaaccag
    10741 taatactaat ttataaattt caaaacaaaa atattacata ttataaatct tctaacttct
    10801 aacttctaac ttctaacttc taacttctaa cttctaactt ctaactaatt ttaacatgcc
    10861 cataaaatat aatacccaca aatgataaat aatactcaaa atgtctattg aaataactat
    10921 acatccttaa ataacttaac ttaaatctaa acatttcaaa aacttcttaa aaatatgtaa
    10981 aaatttgatg ataaagtagc gattttttca aaaaaaatgt aatatagagt tatatcttat
    11041 aatattttag agataaattt tatcatagtg gggagaaatt tgtatggatt taagcaaaaa
    11101 agtaaaaatt ttagaaaatg catcagatgg caaaagcgaa tttgagatat tagaatttga
    11161 aaaacttgag ggagcttcta gcccattaat ggcaatggga ttatattttg ctagagaagc
    11221 tggtttgaaa gctagacaaa ttaagataaa attaaataat tcatcaataa aaacagaagc
    11281 tggtgcatta tactattata aaggaaatat atcttctgaa actaagattg gtggtgttgg
    11341 aggtcttttt aaaaaaggaa tatcaggagc acttactaat gaatcaatga tgaaacctgt
    11401 atacaagggt actggagaag tttatttgga accttctttt agacattatt tatttatgag
    11461 tcttgataat gattctataa ttgttgataa aggattattc tattgttgct ctgatacaat
    11521 agaagtaaaa gcatttacac aaaagaactt ctcatctgct ttacttggtg gtgaaggctt
    11581 cttccaaata gaattaagtg gtactggtgt aatcgttctg gaatgtgttg ttcctcaaag
    11641 tgaaattgta gagtatgaat tgaaaaacgg tgaagaatta aaggtagatg gtaactttgc
    11701 aatagcaaga acatctggtg ttaatttttc tgttacaaag tcagataaat ctttaattgg
    11761 ttctgcttta aatggtgaag gtttcttaaa tacatttact ggtaatggta cagtttggct
    11821 agctccaact caagtaatgt atgaaaaact aagttttgga acaatgccta ctaatgataa
    11881 tatgaataat aaagtttcta agcaaaaata gttgttttat aaatatttat taaatcatta
    11941 ttgagtttat aaagtcacta taaattttat tagattatta ttataaaatc agagtgtgga
    12001 aatgtttatg tgtattcttt aaataaaaat ttccacattc tgtaaagtta ttatacttga
    12061 tatataaaat tcaatttagg aacatagtct aacattcata aacatattaa agtaatgatt
    12121 tctcttggta attattctca tgccttattc tttgattata tttatcttat tcctagctct
    12181 tatgtcatat tatatttctt atcttattat ttaaattagc attaacttat ctaaaatctc
    12241 gtattttttt aatagtaaag tttacatcta tattaagttc ataaaataat caaggtaatc
    12301 aagagattca actttatttg attttaaaaa tttaagtgaa cttgttctta tacacatctt
    12361 tttgaaatta tatctgtaga aaatatcatt tagagctata attaaattaa cttattaaat
    12421 tttagaaaat ttattaaaaa atataagtta aattaatatt tttaaagatt cttttttata
    12481 actactagaa tagtaattta gtagttattg tatctctaaa gtttaagcat ggatagatat
    12541 acatttgata atttgcctat aatttaccta ctacttggag tattggagca aatacaatat
    12601 attaataata agttcctaaa accaataaat taactaatac acataaagat attgatgttg
    12661 attatacaac tataagaaac ggatttaaca gaatttaatc aagctaagaa attacatata
    12721 tcgtgaggtt tttaaattag aaggtaattc aaaattagtt tatagtcttc tgtgaatcca
    12781 ataaaaagta cagggtacaa gaattgttag taaaattttt aaagatttta tggataaata
    12841 taaatagttt tacttattgt ctaaaacttt aaaataccat taattacctt aaacatttta
    12901 ataggaggac atatgaattt aaataaagtt aaatttactg aagaacatgc tattgaagtt
    12961 attaattgga aatatgaagg taaatattca atatacaatc tacctccatg ggaggaaatt
    13021 aaaaagaaga atttttcact atcaaaagaa gataaaagaa aaaattttat aagttttata
    13081 aacgacaata aagaattgat aggttttata aacttattag atgaaggaag ttctgtattc
    13141 tttggtattg gtataaaacc agagtattgc ggaatgggaa ttggtaaaga aataattagc
    13201 ctaggattac aagaatgtag aaataaatat agtactaaac caattgtatt aaatgtgaga
    13261 atctggaaca tgagagctgt aaaatgttat gaatctcaag gatttaaaat agttgagact
    13321 aaggtacaga aaacgcattt aggggatgga gaattttttg ttatgaggta tcaatagttt
    13381 ataacaatag tcctattgtt aatacaacaa ttaaaatgaa aaaatacatg tatgtaggtc
    13441 atggcaataa gtgtctttga aatataaatt tgagagcttt tgataaaatt ttgtatacat
    13501 atatatttta tactacggat atataaatct atctacatat ataaagagat tgtaagtagg
    13561 tttgtgtata taaaaataga tttatatttc ataaatgtac tttttatttg tattaccttt
    13621 attttatcta agctctcatt ttattatcta atcttccttt ttattggttt tattaagaga
    13681 gatgcaatta tcaacacaac acttccaagt atcagccaat atttctcatc aaaactacct
    13741 tgactcatag acattataaa aaattttcca acatacttgc ctttagtcat catatttaaa
    13801 attaaatatc ctatagatat catgtaacct atgattgtat tattaaataa atttgagaaa
    13861 aacattccta aacttcctat aaacatagct gatgcgaaac ttccagtaat atactcaact
    13921 actggaaaat tacatccaag atatattaaa acatttacaa aacctatgat taataccact
    13981 atacctataa ttgacatcag tatcctaatg aattgtacaa taacttgaga taatgatttt
    14041 gaggatacta tctcatcaat ttcttttgat tgctctagtg aaaatacagg taccaacatt
    14101 accatcccta ttattgcaac ataattttct aaacatatag ctgttgaaat tctatctaaa
    14161 ttagctacac cttgtattaa aggaatcata catagcaata taattgaaca tattaaatta
    14221 gttaaagtgc ttaatttgaa gttttctttt attagaacta aacagttttg aaaactttga
    14281 ccaaacatca agattccccc ttcttttttg ctcatataag aatatagcta acaaaacaag
    14341 caccaatgct cccaagctta tcacaattct attaatcata atttgattga agttttgtat
    14401 aataatatct ccatttaata cactgttatg tcttatcatt agtcccattg ttgaataatc
    14461 acctttgagt cctgtagaat ttatactgta aaaccaccaa attaattgca caataattgc
    14521 tactggtgta tctgttaata tagtaatact cattcctaca gctgtaacta tcataagagt
    14581 tggtagtatc caccataatg tatattttat aaatgctaag ttatcaatag atataccttt
    14641 atctttagca aagaaaatca aaggaagtaa atctttcata cctaaaatta atactggcaa
    14701 tatcatcata aagacaagtg caaaatatct tgataaaact attttgcatg aagatacttg
    14761 ttttgaataa ataatctctc tcattcttga acgcttatct ttaagtgaca taaaaactgc
    14821 cacaaatact ggaaatatac ttaaaactat acctatataa tcacaaaaaa ttcttgcata
    14881 cccattacta actttatctt tttgtattgt atagttgtaa ttctgcatag cttcattata
    14941 ggtaactgga actactccaa atctttgtaa cttgtcaaaa gcatatgcag accctcctcc
    15001 taaaatgtca tctactcttt gcattatttc ttcaaacctt tcaaaagata ctttagaaga
    15061 atctattttt atactttcac cagttttcca aattaaattt atttcactaa tacttttact
    15121 agttatttct tctatatatt tttctatggt cttcgtttta tttttgtcta attttatata
    15181 cttagctact cctgttggat acgttgtata tgtgttttct tgatattctc taattaggtt
    15241 tatagttgca tttggaatga ctttttctgt tttcatagat tctttttgac catatggata
    15301 ttgattgtgt acagagtaat attcctcaat attctctact ttttgtggag caactaatgg
    15361 atttccatca ttaggaatat ttttacttac atcatttgaa actgaactcc caagttgaga
    15421 aatatagaaa aaaactaaaa ctacaataaa aatataatat accatactct ttaatatatt
    15481 tttacattct tttataaata gattcatata ttatctcctc ccattataag catatagcca
    15541 tcttcaatag ttggattaca atttactgct gtctcaaaag gtttatcatc tgatataatc
    15601 ctaagtgtag cctttccacc atgagtcaac attcctataa ctataaatct actttttata
    15661 ttttcaatgt cttttttatc tacttcaata ctatatactt tcccttcagc tagttttgct
    15721 aatgcttctg ttgagccatt gtatatgact tttccactat ccaatatagc tacgttttca
    15781 catgtagctt ctatatctcc aacaatatgt gtagataaaa taacgattct atcatttgca
    15841 atttcgcata gtaaatttct aaatcttata cgttcttctg ggtcaagacc tgctgtcggc
    15901 tcatctacta ttaaaactct gggattatga attaatgctt gtgctatccc aagccttcta
    15961 ttcatacctc cagataaaga ttttactttc acattttgat aattagtaag gtttactttg
    16021 cttaataact ttgctataat ttttcttctt tttcttagtg gtatatccga taaaactgct
    16081 aaataatcca ttgctttata tactgtcatg ttaggataca ttgaaaaatc ttgtggcaaa
    16141 taaccaacca tatttctaat ttccttagta ttctcaatgg gaactccaca catatgtatt
    16201 gaaccttctg tttttgataa taaagtggct aaaattctca taaatgtggt ttttcctgct
    16261 ccatttcttc ctaaaagacc aaacatacca ctgtcgattt caagatttat attatcaaga
    16321 gcttgttttt tcccaaatct tttactaaga ttggatactg ctattttcat ttccatatat
    16381 aaatacaccc ccaaaaataa ttttatagaa ttttattttt ataaatttct tactgtattt
    16441 tcataataaa ttctaaaact taagatatta tttataaatt tctaaatttt ttctaaagat
    16501 tttaattttt ctgtttttaa ttaaattaaa gtatctttgt ttctgaaaat ttttaagttt
    16561 atgaagttat aatttaacat atatttattt tatttgtgag tctattttat atataatgaa
    16621 aaatgtgata agtatccaaa agaattactg gatataataa gatagggtat ttagcctttt
    16681 aagataaata aaaatatctg caactatata ttgcaaaaat ttaaaactaa tttatatatg
    16741 ctaatataaa tatttataaa gacataagac aaatttacct acagatttaa atttaactta
    16801 tatattttaa aaggctaata taatatgtat tttcttctat acaatcaaaa ggcttcatat
    16861 ctaagatatg tgatgacatt aatattcaca atttttagta attatcacat tataaaaaat
    16921 attacagcta gtccatcatc agatgcttct tgtataaact ttttaagctc aattagattt
    16981 gcccatacat attcaaaaaa atcatcttca ctctcatcat tcataacagg ataaatatta
    17041 ttttctaaca tctcattaat attaaatctt tttctaaatg cttgttcttc caaactgttc
    17101 attgcttttg atagtttttt tacatcattt gctgtaatca acatgacagg aaattctcca
    17161 tcaatttcca taagtatgtt tccacttagt actaatttgc taaatatatt ctcatcatct
    17221 ccaccaaaag gacaacctgt caaagtaaag tgaatggcat gccatgcttt atcaatattg
    17281 atagtactat tttcgttggc atcttcaaaa acaaaatctt gtaaattttc ttttgtttgc
    17341 tgtaatttaa aaacattctc ttcacttatt ttagtataac atcctatcat ccccataatt
    17401 acattcctcc taagtagcgt ttttttaatt gtaacttata attaaactta gttacaataa
    17461 aatatattga gaaaagtatt atttcatctt aaatttaaat acctatatta tcaactaaaa
    17521 ttctgtgttt atagcgtaag tattattatt ttttcctaaa aattaaatga atcagaagtt
    17581 ctataggcaa tattgactat aaataagaac tattactttt aaaaaaaaat ttaagaaatt
    17641 atactgtaga atcatgctct acttattagc ttcaaataaa aaataaatca tgttctcaaa
    17701 ttaaataata aataacaata aatttaaatt ttaaactata agagtttctg aagtgttaat
    17761 aagcatacca ataattatat gtgtaagaat ttgatttaat agatatgaat aatttttaca
    17821 ttaattataa atatattaac ctattgt
//
LOCUS       DAEMAE010000064        17636 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEMAE010000064 DAEMAE010000000
VERSION     DAEMAE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.065"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(140..1390)
                     /locus_tag="KPH93_001714"
     CDS             complement(140..1390)
                     /locus_tag="KPH93_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG1688540.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1422..2363)
                     /locus_tag="KPH93_001715"
     CDS             complement(1422..2363)
                     /locus_tag="KPH93_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG1688541.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2603..3679)
                     /locus_tag="KPH93_001716"
     CDS             complement(2603..3679)
                     /locus_tag="KPH93_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG1688542.1"
                     /translation="MSKKLFIPGPIDVREEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATMPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4052..4567)
                     /locus_tag="KPH93_001717"
     CDS             complement(4052..4567)
                     /locus_tag="KPH93_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG1688543.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4931..6001)
                     /gene="leuB"
                     /locus_tag="KPH93_001718"
     CDS             complement(4931..6001)
                     /gene="leuB"
                     /locus_tag="KPH93_001718"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG1688544.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVEICRKNEAVLLGAVGGAKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDVVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGKLIVNEI"
     gene            complement(6008..6499)
                     /gene="leuD"
                     /locus_tag="KPH93_001719"
     CDS             complement(6008..6499)
                     /gene="leuD"
                     /locus_tag="KPH93_001719"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG1688545.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDN
                     FISKVKEGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6514..7791)
                     /gene="leuC"
                     /locus_tag="KPH93_001720"
     CDS             complement(6514..7791)
                     /gene="leuC"
                     /locus_tag="KPH93_001720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG1688546.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDDIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7748..9490)
                     /gene="leuA"
                     /locus_tag="KPH93_001721"
     CDS             complement(7748..9490)
                     /gene="leuA"
                     /locus_tag="KPH93_001721"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG1688547.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLINTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIAVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCENVSCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIREIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9613..9859)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10007..10453)
                     /locus_tag="KPH93_001722"
     CDS             complement(10007..10453)
                     /locus_tag="KPH93_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688548.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFMYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10474..11193)
                     /locus_tag="KPH93_001723"
     CDS             complement(10474..11193)
                     /locus_tag="KPH93_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG1688549.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDIQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11311..12585)
                     /locus_tag="KPH93_001724"
     CDS             complement(11311..12585)
                     /locus_tag="KPH93_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG1688550.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12785..14152)
                     /locus_tag="KPH93_001725"
     CDS             complement(12785..14152)
                     /locus_tag="KPH93_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1688551.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14402..14866)
                     /locus_tag="KPH93_001726"
     CDS             complement(14402..14866)
                     /locus_tag="KPH93_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG1688552.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(14871..15500)
                     /locus_tag="KPH93_001727"
     CDS             complement(14871..15500)
                     /locus_tag="KPH93_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG1688553.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLRKAKEAIFYPIVDFSEEDLKTFFDNCIMKKINESIKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15534..16421)
                     /locus_tag="KPH93_001728"
     CDS             complement(15534..16421)
                     /locus_tag="KPH93_001728"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG1688554.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDSIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16450..>17636)
                     /locus_tag="KPH93_001729"
     CDS             complement(16450..>17636)
                     /locus_tag="KPH93_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688555.1"
                     /translation="LFILVVITYLIITKIFFSKTTIFYDLNNTYDVLLDTDTGVLFNL
                     NVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYGFGLICLQIITSA
                     MSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYSQFSLIFFIVIAN
                     KMKGKNSYLVELAAIPLFGITISNIYLYFFYMIFEFKLKIGKMLKHLITFILMAYICV
                     VSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLLYSIFYFPGASIKKGLFLQ
                     NGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIIIFNLTLHGIVGYNLVNSSI
                     MAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIVISNINGFIEILNIGIKSY
                     PV"
BASE COUNT         5661 a         3238 c         1825 g         6912 t
ORIGIN      
        1 atcccaaaat tatccatttt tcaaaaaagg taactattat taagaatatc aacttatata
       61 atttggattc cttatacgat tttcaattaa aaaaacaagg gctaaaaagt gaattgtaca
      121 ctttttagcc ctttattaat tattcattta tttcatgtaa gaatagccca catcttacct
      181 taggttcaaa ccatgttgac ttagctggca ttatcttatt tgcatctgct actcttatta
      241 aatcttcaat actagtagga tacattgcaa atgctacttt catatcttca ttaactcttt
      301 tttctaattc ttcaagacct ctaattccac cgataaaatc aattctttta tctactctag
      361 gattgtcaat tcctaaaata gcatcaataa catagtcttg taatatagaa atatctaatg
      421 agtcaacagg gtcttctatt tctaataaat ttttatttgc tttcatttta taccacttat
      481 cacctaggta cattccgaaa gttccttttt tgtcaggttt atatttttct tttccttcta
      541 tttcttctag ttcaaaattt tcttttattt tatttataaa ttcttcctcg cttaaaccat
      601 ttaagtcttt gataacccta ttataatcca aaaccattaa attctcatct ggagcaatca
      661 ttgctatgaa gtaattaaat tcttcttttc cagtataatt tggattttta gctctcattt
      721 cttttgctac attttctgct gaagctgtcc tatgatgacc atctgcaatg tatagataat
      781 ctaatttttc aaatttatct actaggttat ctattatatc atcattatca atcttccata
      841 ctgtatgctc tatcttgtca tctgttatga aatcatataa aggttcatta ttatccataa
      901 agtcattgat tatatcatct atcacccttt gatgtttgta agttacaagt atagttcccg
      961 tattagcatc acagtatttt atgtgtttag ttctatcaat ttccttttct ggtcgagtgt
     1021 actcatgttt ttttattgtt ccattcaagc tttcatcaac tgatatacat gctactatac
     1081 caacttgagc tctaccatcc atgatttgtt tatatatata tatacactct ttgtcatctt
     1141 ttactagaat ttcttgtttt cgaaactcat ctaaattttc tcgcgctttt aaataaactt
     1201 tttcatcata taaatctata ttttcatcta aatcaatttc tgatttatct atatgcaaaa
     1261 atgaatatgg attcccttct gcaattcttc tagcttcttt actattcata acatcgtaag
     1321 gaagtgctgc tactttatct actatatatt tatttggtct aatcgcctta aatggtttaa
     1381 ttgttgccat tactaatttc ctcctaatta tacaaaatat tttataatgc tacccctatt
     1441 agattgttga agtcaagaaa gaaatcttga atcacattta ctatttcgtc tcctattctt
     1501 tcttgagctt ctactgtaga tgccccaata tgaggtgtag gagaaactct tggatgattt
     1561 actaagtcta aatttaatgt aggttcttgt tcataaacat ctatagcagc tgctgataat
     1621 ttaccttcat ctaatgcttt tagtaaatct ttttcatcaa ctaaaccgcc tcttgcacag
     1681 tttattaaat aagccccttt cttcatctta tttatttctt tttcagttat tactgccccc
     1741 ttattcttat caaatggtat gtgtaaagat aagaaatctg cattttctaa aacttcttcc
     1801 atatcacaga atttataatt gttaaagcct tgcgcctcac ccattatgtc agtatatata
     1861 acattcatac ctaataattc tgctctcttt gcaacttctt ttgcaattct tccaaaacct
     1921 ataagtccaa gtgtctttcc attaatttct gtacctttat attttttctt ctcccacttt
     1981 ccatctctca tagttacatt tgcagtattt atgaatcttg caagaacaaa taattgacct
     2041 attgtaagct ctgcaacaga tatactgctt gcatttggag tattcataac ttttatgcca
     2101 ttagcttgag catatttaac atctatatta tccagtccta ctccacctct tactatcaac
     2161 ttcaactttt taccctctaa tgctttgtct attacatctt tagtaacttt agtagctgaa
     2221 cgtactacaa gtacatcaac atctttaagt ttatctccta aaacatcttt ttcataaaac
     2281 tgctctatca cattaaaatc taactctctc aattttctcg ctgcttcttt ctctattcca
     2341 tcagtaacta atatattata cataacaaac tcctccaatt tttattcact taatttttcc
     2401 aatttttatt ttttaaagta ttttcatcaa taaaaaactt tacttaattt aactttctgt
     2461 ttatataatt ttcaaaatat ttttttatta aagtcatatc aaatcttcct attgtttgtt
     2521 aaaaatttaa tatgtaattt ttataaaata ttatttatta tatattctaa atttatctta
     2581 aaacatacat atttttgcaa tattatttat taaaacctaa tatatcatca atattatcta
     2641 ataattcttg tacatcttcg acagtatagt cacccatatg tgaaattcta aaagtttttt
     2701 cttttaaatc tccatagcca tttgctattt ggaatcctcg ttcttgtaat tttgaattta
     2761 aatttgatac actaatacct tgagtatttt ctataacagt aagtgtattg gataagtgat
     2821 tttcattggt aaaaatttgg aagtgttttt tagcccagtt tctaactaag tttgccatgt
     2881 cttcatgtct gttgaatctg ttatctaggc cttcatctaa tatattatct aattggaaat
     2941 ctaaagcgaa catgtgagaa agagatggtg ttgaagggta ttggtagttt ttcttgatta
     3001 agtattcata cattgctaac aaatcaagat atactcctct aaaaggtact tgcttagctc
     3061 tatcgattgc tttttgagaa aatgtgcaaa tagacattcc tggtggtaat cccagtgctt
     3121 tttgtactga tgttatacaa atatctattc caatcttatc aacctcgact tttattccac
     3181 cggctgaact tacagtatct acacagtaga ttacatcttc atacttttta acaacttctc
     3241 caatttcttc tattggattt cttattccag ttgaagtttc attgtgagtt acagttatta
     3301 aatcgtattc tccggtagaa agcaccttat caaccatttc tggcattgtt gcctctccta
     3361 attcaacttt aaatatatcc gctgggacat tattagccac tgccattttg taccatctat
     3421 caccaaagga tccacaggaa aatactgctg cctttttgga tgtacaggaa cgaatggagc
     3481 cttccattaa accagttccg gatgacgtgg ataaaagtat cgtgttgttt gtatagaata
     3541 gtttttgcat cttttcactt atactctttt ggagcataga tgcatctttt cctctatgtc
     3601 ctatcattgg tgtagccatc ttttgcaaga cttcttctct aacatcaata ggaccaggaa
     3661 taaataattt cttactcatt ataattcccc cctttttagt ttgtaatatg gatagcgttt
     3721 ttactgtcct tatttttaat aactataata tcaagctgaa ttaactatgt caacataaaa
     3781 tttatttttt atatataatt tattttttat attttatgaa aacattcatt ttccaccttt
     3841 tcataagtat gaatattctg aattattttc tcaaaaaaaa tatacttttt atctatataa
     3901 attaattttt ttagtatgta aatttaacgc tattacgata aatcttttta gtataactaa
     3961 tttattttta tcttgtattt ttattgtatt tttgaatttt attttttcta aatataaaat
     4021 ttatgtttta aaatataaaa tttttattta atcaaagttc aatatattcc actttttctt
     4081 tcgattcttc aaaaaaccat tcttcattag aaaaatcacc ttctgcaatt tgtccaatag
     4141 gcattttcat atactttcca gttgctgtca ctgctatatc tccatttggt agaattattt
     4201 ctcctgtacc ttcaaataat tttctgtttt ctttagttat tcttccaaca acttttatag
     4261 tagcatctgt tggtactggt ttcttatatt tcaattctaa agatactgtt actccccata
     4321 tttggtcatc atttatactt acagctcttc caatagtctc atccaatatt gcagcagaca
     4381 tacctccatg aactctccca ggataacttt gatgccaatc ctttgtatta caaattgata
     4441 caagctctcc attttcaagt tcataaaaat ctgctttgag tcctaattca ttttgtgttc
     4501 cacaaactaa acaagattta ctactatttt gcttcttcaa aactttatac tttgccaaaa
     4561 aatccatctc ctaaccgaca ctagtagtcg cttaatatta aataattcta aaatatatta
     4621 tataatatta ataaaatccc tgcaaacttc taataataaa aatagactac ttcaatatga
     4681 taagtttaat ttaaaatatt attttctaaa aaaataactt tatcaaaata gaaacttaaa
     4741 gtagagatag acctcttttt cgttattaaa atagtaaaaa tatatttgac tcaatataaa
     4801 aaagggtgtc tcattaacta ataaagttta ttttgagaca cccctcataa aacaaattat
     4861 attacaatag atttgtacta attttacaag ttatatattt aaattatatt attcaaatta
     4921 aactataatt ctaaatttca tttactatta atttgcccat ttctcttgtt cctacaacat
     4981 ttcccacacc attatagata tcaatagttc tataaccttt attgagtaca ctttgtacag
     5041 catcttcaat acacttagcc tcttcttcta agttaaaact atgtcttaac atcatgctta
     5101 cagatagaat agttgcaatt ggatttacaa catcttttcc agctatatca ggcgcacttc
     5161 catgtattgg ttcatacata ccaaaactat cttctctaag tgatgctgat ggtaacatac
     5221 ctatagaacc tgtaatcata gatgcttcat cagatattat atctccaaat atgttattag
     5281 ttactattac atcaaattgg ctagggtctt ttacaacttg catagcagcg ttatcaacat
     5341 acatatgtga cagttctaca tcttcaaact ctttagctaa gtcttccatt atacttctcc
     5401 aaagtcttga actctcaagc acatttgcct tatctacact tataagtttt ttatttctat
     5461 ttctggctat ttcaaaagct ctcttaccaa ttcttcttat ttcattttca ttatatattt
     5521 ccacatcata agcattttca ataccatcaa tgatttttct tccacgttct ccaaaataaa
     5581 tgccccctgt taattctctt acaacaacaa aatcaatgcc tttctcaact atatccgttc
     5641 taagtggtga agcttctttt atactttcat acattgtagc tggtcttaag tttgcaaata
     5701 agcccaatga ctctcttaat tttaaaagac cagactctgg tcttttatct cccttacatt
     5761 tatcccattt tgctcctcca acagcaccaa gtaatacagc ttcgtttttt cgacaaattt
     5821 caacagtttt attaggtaga ggttctccag tttcatcaat tgcacatcct ccagctaata
     5881 catattcaca atcgaatcta tgattaaatt tacaacaaat tttatttaga acctttatac
     5941 cttcatctat aatttccggt ccaacaccat caccttttat gactgctata ttacaattca
     6001 tactccacta ccccctattt gcttttattg agttaattaa gccttcattt aatataatat
     6061 tttgcataaa ctctggaaat ggttctgctt tatattcttt tccttttgtt atatttttta
     6121 tcacacctgt agaaaaatct acttctatat tatccccttc atctatgtta ttggcagctt
     6181 cttcgcattc aagtatagga agccctatat ttatagagtt tctaaaaaat attctagcaa
     6241 aagtagaagc aatcacacag gatactccac tttcttttat tacaattggt gcatgttctc
     6301 ttgatgaacc acaaccaaag tttttagttg caactataat gtctccttct tttaccttag
     6361 agataaagtt atcatcaata tcctccatac aatgcgttgc tagttcttta taatcagcta
     6421 tatttaaata tctagctggt attattacat ccgtatctat attatcaccg aatttaaaaa
     6481 cacttccatt ggcaatcata aattttacct cccttaaatt atttctgatg gttttgatat
     6541 ttttccagtt atagctgatg ctgctgctac agctgggctt gcaagataca cctctgaatc
     6601 aacatgcccc attcttccta cgaaattcct atttgttgtt gaaatggatt tttctcctgc
     6661 tgcaagtata cccatatgtc cccctagaca tggaccacaa gttggtgtag aaactgctgc
     6721 tcctgcctct ataaaagtgg taatataccc tttttctata gcttcaagat atactttttg
     6781 agttccagga aatatcataa ccctaactcc atctgctact tttttgcctt tcattatctc
     6841 agctactact tcaaggtctg atattcttcc atttgtacaa gaacctatta caacttggtc
     6901 tatctttata tccttatcta tatcatctac tgttctagta ttttcaggaa gatgaggaaa
     6961 ggctactgtt tcttttagtt cagctaaatc tatttcgtat acttcatcat agactgcatc
     7021 ttcatctgct tcatatatat ttacatcttt tgttatagtt gaacacttat gtgaatttat
     7081 atattccata gtcttatcgt ctactggaaa aattccattt tttgctcctg cttctattgc
     7141 catattgcaa atagtaaatc tatcatccat agataagtat tctactccat ccccgcaaaa
     7201 ttccatcgac ttataaagtg ctccatcaac acctatctca cctattatat gaagtattat
     7261 atctttacca cttatccatt tatttggttt attcttcaaa acaaatttta ttgctccagg
     7321 aaccttaaac caacattttc ctgtagccat gcctgctccc atatcagttg aacctacacc
     7381 agtagaaaat gctcctaatg ctccatatgt gcaagtatga gaatcagcac ctataattac
     7441 atctccagca gttactatac ctttttcagg aagaagaaca tgctctattc ccatatctcc
     7501 tacatcaaaa aaatttacaa ctccttttga ttttgcgaaa cttcttgtaa atttacattg
     7561 ttctgcactc tttatatctt tattaggtgt aaaatggtct aaaaccatca ctactttagt
     7621 cttatcataa acatcttcaa ttcctaattt ttcaaattct ctaatggcta ctggtgatgt
     7681 tatatcatta cctaaaacta catctaaatc tgcttcgatt aattgccctt tcttaacctc
     7741 atttaattta gcatgtttgg ctaatatttt ctgcgtcata gtcatcccca ttttttattt
     7801 cctcctcaat ataactttta tttattgcat tcataagtgc attaatagct gctgtattta
     7861 tgttttccga tatactcaca ccaaaataga cttcattatt gtgctctatt cctacataag
     7921 ctacccctct tgaatgtgac ccttcctcta atgcatgttc ccagtaatat ctaaatttat
     7981 aatctttcat gccacattgc tttagtgcat tattaaatga atctacaggt ccatttccta
     8041 taccaacaat cctttgttca tttcccatat aacttattct tgcagtcatc tcaatcatgt
     8101 tagtatcatt agaatcatca ttttcatagt ttaattcatc cattgatttt attttatact
     8161 tcttaacttt ataagggctc tcaacttcaa tatattcttt cttaaataaa ttgaatattg
     8221 cttcactact aagttcttcc ccaattctat cagattccat tttgactaca tttccaaaat
     8281 cagaatgcat attcttaggt atcataaatc cataatctgt ttccattata taaacagctc
     8341 ctccttttcc agattggcta tttatcctga taatttcttt gtattctctt ccaatatcat
     8401 gagggtctat tggtaaatac ggaacttccc agtaatcatt atctctgttt ttcattgcta
     8461 tcatgccttt tctaatagca tcttgatgtg aacctgaaaa agcacaatgt acaagattac
     8521 ctgcatatgg atgtctatca tgtaccataa gttttgtaca gtcttcatat atttctctta
     8581 tcttatctat gttgctaaaa tctagctctg ggtctatccc ttgtgtataa agattaagtc
     8641 ccacatttac tatatccata ttcccagttc tttcaccatt tccaaatagt gttccttcaa
     8701 gtctatcggc accagccaat aagccaagtt cacttgcagc cgtacaagtt cctctatcat
     8761 tgtgagtatg caaacttagt ataatagaat ctctacaact tacattttca caaaaccatt
     8821 ctatttggtc tgcatataca tttggtgttg ccatttccac tgttgatgga agatttatta
     8881 ttactttctt ttgaggagtt ggtttccaaa cagctatcac tgcctcacat atttcaagtg
     8941 cataatccat ctctgttcct gtaaaactct ctggtgaata ttcaaaagta aattctgttt
     9001 ctggatattt ttttacttct tcattaaata attttgctcc ttcaactgct atatctatta
     9061 tttcttgttt actcatatta aaaaccacat ctctttgaag aacagatgta gagttatata
     9121 aatgtataat tgctttctta catcccttta ttgattcaaa tgttttttct ataagatgag
     9181 ccctagattg tgtaagtact tgtatagtta catcatctgg aatcatattc tcatctatta
     9241 attttcttaa aaatgtatac tctgtatttg atgctgatgg aaatcctacc tctatttctt
     9301 taaatcctgt attaataagc attttgaaca ttctgacttt ttcatttaca ctcataggtg
     9361 ttggcagtga ctgatttccg tctcttaaat cgacactaca ccaaattgga gccttaaata
     9421 tttcattatc tggccattta cgatttacta tttgcatttt atcatatttt ttatactttc
     9481 cacacttcat gttatttccc ccttttttgt tttttgtatt cagtatacct tatatcgacg
     9541 caatcctgca caattcataa gaataaatgt caattttatc attagaataa aaataacctc
     9601 atctctaatg aaattagaga cgaggttttg ctcgcggtac cactctattt agctttctaa
     9661 aaagctccct tagtaatagc caatacaata attataatga ctatatttcc tataacgtgg
     9721 aattactccg attattctaa gatttaaatc ttagaaaaca gctcctagac aagttcagaa
     9781 ctatcaacta ctaaatcaca ccatccttta gctctctgaa agtttcattg ttcttactac
     9841 ttctattcat cactttttta atcatttaaa ttttctaaat attttgattt aatagttatt
     9901 atatacacat taaaatgtat tgtcaacaca aaaaataatt atctcaattt tttatttttg
     9961 ataagcaatt ctataaaaca tgctaactat tgaaaacagt atgatattat gccttaaact
    10021 ttttcatctt ttcatctata atattcaata attttttttc atcttccaaa ctcaatgccc
    10081 ttaatctaat tttataatta gattcatcag tgtatatatt taatatattt tttactactt
    10141 ctattttttc tattatctta taattaaact tttgactttg tttagatttt atactgaata
    10201 tacattgatt atttattaat attgaatgat tatcgagagt aatatacact tttccctcat
    10261 atttatttga aaaatcacga ccttgtttaa atagaaaaac tgctgttatt atacataaaa
    10321 tatataatat tgtcgttgaa tacataaaaa ttgtagctaa aattataagt gctagaacca
    10381 tatatatagt cattcctttt ttaaaataac cattgtcata ataattcata tagataaact
    10441 tatttttact caaatttttt cactcccaag attctaaaaa tttatatcaa aaaattctat
    10501 aatttcatct tcatgataat ttatgactaa ttcatctgga aaatctactg cttctaaaac
    10561 tttttctgca ttttcaaatt ctcctataga ataacatata tgagagtcag taccaagaat
    10621 tactcttgca ccatatgttt tacacaatgt caacatatga cttacatttt cccaagtacc
    10681 cgttctatga gagtttgaac ttaatgatga attatttatt tctaaaagga tatttttatc
    10741 tttagctttt tttactatag gttcataatc taaaggatac ctactgtcat ctggatgtcc
    10801 aattattttt accttaggtt tatccataac ttttaaaata gcatttgtat tttcttcttt
    10861 tgtgcctgaa gctatacaag gtctatgcaa acttgcgatt atataatcca aatgttgcaa
    10921 catatcttct tgaatatcta aattaccact ataatccatt atatttcctt ctatccccct
    10981 aagaatcttt acaccttgca cttcacgtgg aagcaaatgc aaattataaa aatagaatgg
    11041 atgtggtcct cctggcatat taggtccgtg gtctgatatt cctaaatatt ttatattctt
    11101 cttactcgcc tcttcgacat tttccttgaa agtactatgt gcatgtccac ttattaaagt
    11161 gtgacaatgt acatccataa tagatttttt cattaaattt cctcctaata ttttacataa
    11221 attcgtgaaa atagctaaag ggctatttca aaactaaagc tgtcataata gacagtgtaa
    11281 cgttttaaaa cagccccatt ttcaattatt ttacacttct aaatctttaa atttatcttt
    11341 aaataattct ccaatacttc ctaaagattc ctctacttct aggtattctg ttatatcctc
    11401 ctctggctct ctagttgctt cttttattga aaccaacatt cttttagtct ttggtttaaa
    11461 atcaagaatc ttaactttaa cctcatctcc aatattaacc acattagtaa ctttagccac
    11521 tctagtatca ctcaattcag atattggtaa atacgcatct acaccttctt taatttgaac
    11581 aaaagcccct ttttctatta ttctaagaac tttagcattg acaacatcat caatttgtac
    11641 ttcttttgct attaaatccc aagggtcatt atttatgtct tttaatgcca aggcaatttt
    11701 tttagtctct ttatttacat ccaacacata aacttctact tcttgtccct ctgaaacaac
    11761 atcttcaaca ctttcaactc ttgtccaagc taaattattt atatgtgcta atccttcaat
    11821 cccaccaata tccacaaatg caccataagg cattatcttt gtcaccttac catctctttt
    11881 ttgtccaact tcaagagaat taaataagtc ttctttagct gctttgatac gagctttttc
    11941 ttcttctctt tcttgcttaa ttcttgctct ttcagcttct ttttctgcct taatttgagc
    12001 tcttctttct tctctctctt tatttatacg ctcttgtaat acatctctat gagatgctac
    12061 aagtctattt tttctaacat ccaattcttt tatataaact tctaagttaa gaccaacaaa
    12121 tttagatgta tctgtaataa attttgtatc aagttgagaa attggtataa aaaatgtata
    12181 tgtattgtaa ttagcaaata cacctttatc tatattcttt tcaacattta cagttattat
    12241 tctatgttca tcataagcct tttgtaactc ggcaaaatca tttcttttgt caagttgtaa
    12301 tttagataaa gttattgtac catctttttg agatactttt gttattacag cagttatttc
    12361 atcacctata tggtatatat cttctatata ttgatttttt tctatgttca attcacttat
    12421 aggtattatt ccatcaaacc catagtctaa tcctaattgt acttcatcat ttctcactgc
    12481 agttattttt ccagttgtca attctccaac tttcactttg ctgaatactt gttcttgctg
    12541 gtcaagtaat tcttgcattg ttaaatcatt ttccattata aacatccttt cctcttattg
    12601 attatcatat ctgtaaatat tataccatta atattgcaaa tacttacatt tttcttacta
    12661 taatttttaa aataaattca acttcatata tttatacatt atagaaaata tcttattaaa
    12721 ttaaaataca aattctaatt tttatctaat aaaatatttt cataaaaaat ttatattgta
    12781 tatcttattc ttttattgct ttaggttttg atagaaaaca tttataaaat ataaagaaag
    12841 aaattgctgt tatagtagcg gctgttatat ccgcaactgg ctctgctaaa attactgcaa
    12901 aaagtttttc attaaagaac gttggaagta taaatattaa aggtattaaa agtacaatct
    12961 ttctaagtaa agctaaaact aaagatattg ttgcttttcc aagtgctaaa aatgtctgtt
    13021 gacaagctat ttgaatccca aacataaaag caccagcaaa atatattttt atactccaac
    13081 tagtcatact aagtaactgc ggattcttat taaatatact aacaaaaaat gcaggaaata
    13141 tcattaataa aagccacaaa atagtagaat atgtaaaaca tgacaataaa cataacttaa
    13201 atgtcttttt tactctatca agttgacctg aaccataatt gtaacttatt attggttgtg
    13261 ctccttgtgt aacccccata ttaggaagca taagaatttg cattatactg ctcattatgg
    13321 ccattgaacc tactgctaaa tctcctccat acatttgtaa tttactattt aaggctataa
    13381 gtactaaact ttctgtagat tgcattataa atggagaaat tcccaagcca agcactggta
    13441 agataatact tgcttttggt atcatatact tcttttttat tttaagtata cttttctttc
    13501 caaagagaaa cataagtacc caaatagcag aaacaccttg tgcaattata gtagctaatg
    13561 cagctccttt tacacccata ttaaagccaa atataaaaat cgggtcaagt attatgttta
    13621 ttaaagcacc tatagcaact gtcatcattc cagttgtagc aaacccttgg gtatttataa
    13681 aagtattcat acccattgaa atctgaacaa acactgtacc tattaaataa atatttaaat
    13741 agtccattga atatccaatt gtagattcgc ttgctccaaa catccaaagt aaaggctctt
    13801 ttccaatctt aaaacctata gtcaaaagta ttgctaaaat caccagtaca gaaaaactat
    13861 tactcattat tttctctgcc tcatcattgt tttgctcacc catttttata gcagctaatg
    13921 gacctcctcc cataccaatt aaataactac atgcagttat tataattatt attggaaatg
    13981 ccactccaac accagccata gcaacttctc cattttccat tctaccaata aaaatcctat
    14041 cgactatgtt gtataaaaca tttacaattt gtgcaataat agcaggtgta gcaagtttaa
    14101 ataataattt acccacactt tcactgccta agttagtgtt tgaattttcc attttaaaac
    14161 ctcctcaatc caataattaa aatgtatcac taaaatttta ttctatataa aaatttcatt
    14221 ttattagcag acaaatttag agtactttcc ctacaagtaa tactatacta tatttctttt
    14281 gccatagtaa acattttaga actaactgaa tttaaatact ttatctcatt ttttatttat
    14341 tattaaaaat ttttcacacg tactcataaa aaaactatcc aagtatatta cttagatagt
    14401 tttatattat atgtaaagaa agagctgaaa ctataacccc tgataacaaa tttccaagaa
    14461 atactcccat aaaagcatct tttattctca tttttaatat ggaagctata gcagatgcag
    14521 tccatgttcc tgtagttggc agtggtatac ctacaaatag tattatacca attatcgaaa
    14581 cactttttaa tttcttcatt cttgagttta tttttctatc aattacatca gcaatccctt
    14641 taaatagttt cttccctctt aaaaattgta atacatgtct aaatgtcaat atcaagggaa
    14701 tcgataccaa tgtagaacct attacactaa atacataaac ccatatagga tttaagccca
    14761 ttgcaatacc tataggaatg gctcccctca attctgttac aggaaccata gataaaatta
    14821 atatgtgtaa atatcccaaa ctattccctc ctattctaag tgacattaag ttatgaattt
    14881 atttgctcta taacctcacc atccttttta ctaactaaaa atgcattttt agtcaatgaa
    14941 agcattggta aatcactctt agagtctgaa tatgcatatg aattttcata atcaatctct
    15001 atcccaagtt tacctaatac agcctttatt ctttttactt tttcaattcc cttacagttt
    15061 tctccaatga attgattttt atatcttgaa ttttcataaa aaaactccgt gcctatgacc
    15121 tcgtctgcaa gaccataata cttaaaatat ttcatatatg catatggaga tgcagttatc
    15181 ataattataa cattatcttc ttctttattt ttgtaaataa ccttttttat agattcatta
    15241 atttttttca ttatacaatt atcaaaaaaa gtttttaaat cttcttcact aaaatctact
    15301 attggataaa agattgcctc ttttgccttc ctcaagggta tcattctaat taaatacata
    15361 aatagaataa aaataatgta tggcaacttt ataatagttt taaaggtttt attttttaaa
    15421 gagtaaataa aaaaatctat catgctatca gtatttataa cagtataatc tacatcaagt
    15481 aataatacac tttttttcat aagtatttcc ccatcctatc actaaaaata tcactaaaaa
    15541 atcaatatta ttaaacatgt aattatccat aagattacgt ttatttttaa agcattatcc
    15601 tttaggacaa tatcagtagg attattttca tttgtagtgt taattatata attatatcta
    15661 agtacaccat aaactacaaa tattgttgta aatatcatat gtggcttttc tggattactt
    15721 gtagaataca atgcataaca tacaattgta gaacttaata taactatcat catttgattt
    15781 agattttcta tgtcataatc ttccaatatt tttctatgtt caactgcatc atcccttaga
    15841 gtttctattt cttttttacg ttttccaaga gctaagtaca gagataagaa aaatgtacat
    15901 aaaattatcc aatttgataa actaacactt atagctacac atcctgcata aaccctaagt
    15961 ataaaaccaa gagatatgga aaacacatct aatagtatta tattctttag tttaagagaa
    16021 tataatatat tgtttatcaa atagagactg agaattatag aaagattaaa actaacaact
    16081 gttgataaac agactgaaat taaagctaat actacaaata gtatattagc actttttttg
    16141 cttattcttc cacttgccac tggtctatta cattttttag gatgtttttt atctttatcc
    16201 atgtctatag aatcattcag cgcatatact gatgatgaca ttaggcaaaa acacacaaac
    16261 gcaataaacg tatttaaaaa taaatttata tttagtatgt tgtttgaaaa aattatagct
    16321 gaaaatacaa agaaattctt tatatattgc tttattctca ttaactttat ataatctgaa
    16381 attttagtaa gaagccttat acttgtattg gtctgtttca taaacttact accctttcaa
    16441 tattattttt taaacaggat atgattttat ccctatgttt aaaatttcta taaatccatt
    16501 gatattacta attactatag taactaataa cagcgataga aatatattat acatattctt
    16561 ttcatttttt ctaagcgctt tcgtaaagta agccaataaa attataactg caaaactaaa
    16621 atttatagcc ataatacttg aattgactag attatatcct actataccat gtaaagttag
    16681 attgaatata attattccca tacatagctt aggaactctt ttttctgaat tttcaatgac
    16741 tgataaaaca caaaaacata aagctaataa tgtaagaatc actggtattg tagcaacttc
    16801 tccattttgc aaaaataacc cttttttaat acttgctcca ggaaaataaa aaatcgaata
    16861 caacagtctc tctataatca ttgcaatctt ttctaaaatt ggttctccac ttataaattt
    16921 cgtatcatac tgtattacat ttccaaggtt cataaaactc tcataagatt tcgttgaaac
    16981 tacacaaata tatgccatta ggataaatgt aattaaatgt tttaacattt ttcctatttt
    17041 taatttaaac tcaaatatca tataaaaaaa atacaaatat atattactaa tagttattcc
    17101 aaatagagga atagctgcta attctactaa ataagaattt tttcctttca tcttatttgc
    17161 aataactatg aaaaatatta atgaaaattg actatatata aatctctcta catttaaagt
    17221 cataaataac tgtggaaaag aaaatatcat tatcatagta agtaaaatta aagttagtct
    17281 ctgcattttt atctttttaa tatgattaaa gactaaagtt attgacatag cactagttat
    17341 tatttgtaga caaattagtc caaacccata agcactatta aaatctgttg tcccaggatt
    17401 tgctattgaa gtacaaaata ggtatattgg atatgcaaaa attgatataa ttgctgaaaa
    17461 caatatgtgc ttactattgt cttgacttat agcaaataca tttaaattga ataaaacccc
    17521 tgtatctgta tctaacaata catcataagt attgtttaaa tcataaaata tagtagtctt
    17581 agaaaaaaat atcttagtta ttattaagta agttattaca actaatataa acaaaa
//
LOCUS       DAEMAE010000065        17191 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEMAE010000065 DAEMAE010000000
VERSION     DAEMAE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.066"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            71..604
                     /locus_tag="KPH93_001730"
     CDS             71..604
                     /locus_tag="KPH93_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG1688556.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVIKNIGNYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPEVEFEVIILRYYHDLKFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            601..1362
                     /locus_tag="KPH93_001731"
     CDS             601..1362
                     /locus_tag="KPH93_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688557.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLNNTDKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICILLFSTSTDSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAITLIYEIRKTWRHLCSFENIII"
     gene            1400..2266
                     /locus_tag="KPH93_001732"
     CDS             1400..2266
                     /locus_tag="KPH93_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688558.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPSSGEVKVDGKNNLDMGEDFRNMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKAKTQELLEMVGLSGVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFILTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLVNIHHSGGVANLRIVSDVKPYDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2287..3528
                     /locus_tag="KPH93_001733"
     CDS             2287..3528
                     /locus_tag="KPH93_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688559.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNDGYITDKKVENSIREYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIKEPYLNGYYQGWNYFLLARDLLIFTIVAVCISIAS
                     VFSGEYQSNADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGTD
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLTIYMILIVLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFFKYSETNKLYNQILRILPSNAISFSFTDYIDYKFGNLIVTLPTMIIFTC
                     LVLTVVFLPFVKNNFKEHQVN"
     gene            complement(3746..4324)
                     /locus_tag="KPH93_001734"
     CDS             complement(3746..4324)
                     /locus_tag="KPH93_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1688560.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKVDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLIARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSVCL"
     gene            4709..5443
                     /locus_tag="KPH93_001735"
     CDS             4709..5443
                     /locus_tag="KPH93_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1688561.1"
                     /translation="MKQNKYDDDKFFNKYSKMERSTNGLAGAGEWHALKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGIDISEKMLKEARNKTQLDNINYMCMPIEDINF
                     PKGSFDVVISSLAFHYIQSFEDICKNISSCLSNKGNFVFSVEHPVFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLIKTGFEILEIIEPQPEE
                     KLLDTIPGMKDELRRPMMLLVSAKKR"
     gene            complement(5586..6077)
                     /locus_tag="KPH93_001736"
     CDS             complement(5586..6077)
                     /locus_tag="KPH93_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG1688562.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            6426..6809
                     /locus_tag="KPH93_001737"
     CDS             6426..6809
                     /locus_tag="KPH93_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688563.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            7314..7697
                     /locus_tag="KPH93_001738"
     CDS             7314..7697
                     /locus_tag="KPH93_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1688564.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            7699..8049
                     /locus_tag="KPH93_001739"
     CDS             7699..8049
                     /locus_tag="KPH93_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688565.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(8143..8601)
                     /locus_tag="KPH93_001740"
     CDS             complement(8143..8601)
                     /locus_tag="KPH93_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688566.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGKADFLYSSKLQKLLKETYINQSRASYMSGCGLFWSSYWDVLEEKFEEWLY
                     NSYIFFDAEDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(8646..9434)
                     /locus_tag="KPH93_001741"
     CDS             complement(8646..9434)
                     /locus_tag="KPH93_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1688567.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAIQIHFNSNVTTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKNRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(9573..10010)
                     /locus_tag="KPH93_001742"
     CDS             complement(9573..10010)
                     /locus_tag="KPH93_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG1688568.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            10848..10976
                     /locus_tag="KPH93_001743"
                     /pseudo
     CDS             10848..10976
                     /locus_tag="KPH93_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11424..11933
                     /locus_tag="KPH93_001744"
     CDS             11424..11933
                     /locus_tag="KPH93_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688569.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIGFLVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLAPIVLIGAII
                     GSFLTRKKI"
     gene            12322..12858
                     /locus_tag="KPH93_001745"
     CDS             12322..12858
                     /locus_tag="KPH93_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG1688570.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLIDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVIIGILHS
                     REISMKEYIYFCYRQFKR"
     gene            complement(13295..14095)
                     /locus_tag="KPH93_001746"
     CDS             complement(13295..14095)
                     /locus_tag="KPH93_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688571.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETALGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGASKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(14085..14837)
                     /locus_tag="KPH93_001747"
     CDS             complement(14085..14837)
                     /locus_tag="KPH93_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688572.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEAISISDKVAILSKRPATIKNIYDIELN
                     MDGTKTPLKTRKVPDFQKYFDILWKELDTHEA"
     gene            15007..16365
                     /locus_tag="KPH93_001748"
     CDS             15007..16365
                     /locus_tag="KPH93_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688573.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            16358..16990
                     /locus_tag="KPH93_001749"
     CDS             16358..16990
                     /locus_tag="KPH93_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG1688574.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGERTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNSDNSVNRDASRRVELKYEININQNK"
BASE COUNT         6512 a         2161 c         2410 g         6108 t
ORIGIN      
        1 tataaataat tataaaacaa taaattaatt ttttatactg acttttttat agtataaatc
       61 gttatatata atgacggagg tgataatagt gaatgatgct cggattatat cacaaataaa
      121 agcaggaaaa aaagaattgt ttgatactct aataaaaaaa tactatgata gcatctactg
      181 ttattgttat cgccatgtag acaataaaat gactgcacaa gatttaactc aagatgtatt
      241 tgtaacagtt ataaagaata taggaaatta taaacattat ggtaagtttg aaaactattt
      301 gtatgttatt gcaaaaaaca agtgtaagga tttttggaag aaaaaacaac caatatattt
      361 agagtcatta gataaggaga tatcaaatca agagttatct gatatagaaa ataaaatgtt
      421 tataaaagaa gctctgacta aattaccaga agtagaattt gaagtgataa ttttacggta
      481 ttaccatgat ttaaaattta aggatattgc taaaattatg gagtcaagtg tctctgtcac
      541 aaagtacagg atgagtaggg cattaaaaaa actagaatta tatctggaag gaggtaaatt
      601 atgaataata aagaaataaa aagaagtcta cagaagtata aggttccaaa atatgattct
      661 tcagctcttg aaaagactat taaaactata catgagctaa cacttaataa tactgataaa
      721 agaatgaata ataaagactt ttttatatct caaattggtt ttataagaaa aagaacttgg
      781 attatgcaat ttctaatact atgtacaata tgtatacttt tatttagtac atcaacagat
      841 agtaattcat tttataatac tttatccttg atttctatag catcaccact ttttgtactg
      901 acaaacatag aagaattaac aaaagtatat aatagtagta ttttagagat agaattttca
      961 acaaaaaata cattacataa aattattgct actagaatgc ttatatttgg aataagtgat
     1021 ttattgttta tgattattat tttggtatct gcaaaacaaa tcattgatgt aaacattctg
     1081 tatataataa tttatatact agttcctttt aacttaatgt gtataggatg tatggaaatt
     1141 ataaatagaa ataaaggcaa gcatacaaat tattattgta tgatatatgg tatatctctt
     1201 gtattattaa tagttacact gagtaacttt agagataatc tatatcaatt tgaagcaata
     1261 aatcaatggc taattgtttt tctagtcaca gcaataacat taatatatga aataagaaaa
     1321 acgtggagac atctttgttc atttgaaaat attattatct aagtaaaaaa taagaataca
     1381 cagtatcagg aggaatagta tggagttaag tgtagaacgt ttaacaaaac agtataacaa
     1441 taaaattata gtagataaaa ttgactttaa attaaatgag ggtatatatg gatttttagg
     1501 agaaaatggg gcaggaaaaa caactcttat gagaatgatt tgcggaatat taagcccttc
     1561 atctggagaa gtaaaggtgg atggtaaaaa taatttggat atgggagaag attttagaaa
     1621 tatgattgga taccttcctc aaaactttgg atactaccca gattttactg ctaaagaatt
     1681 tatgctatat atagcatcat taaaaggatt aatgcctagc tatgcaaagg caaaaacaca
     1741 agaattatta gaaatggtag gtttatcagg agtaattaat aagaaaataa gaacattttc
     1801 tggaggtatg aagcaaaggc taggatttgc tcaaacatta cttaacgacc ctaaaatttt
     1861 aatattagat gagccaacag cagggctaga ccctaaagaa cgagttagat ttcgcaactt
     1921 aatttcagaa ttttctaagg aaaagattgt tatcttgtct actcatattg tgtctgatgt
     1981 agaatatatt gcagataaaa ttttaataat gaaaaaagga agatttattt taactggctc
     2041 agtagaagaa gtaacttcaa agataaaaga taaagtatgg aaatgtgtgg ttaatgagaa
     2101 tgaaatagat tatatgtctg atagatattg tttagtaaat atccatcatt ctggtggagt
     2161 tgcaaatctt agaattgtaa gtgatgtaaa gccatatgac aatgcatgca atttaaaacc
     2221 aacattagaa gatttgtatt tatattgttt caatgaagta ctataaatat acataaggag
     2281 ataggtatgt ctacattaat taaatttgag ttaaaaaaaa taataaaaag aaagtctaca
     2341 atagcagtta ttttaggaag tttattctta agcatcattc tttttgcatt aatgccaatc
     2401 caggaaaaag ccaatgatga aagtggaaaa gagtataggg ggctacaggc tattgagtta
     2461 aaaaaacaac aatctaaatt aaatgatgga tatataacag ataaaaaagt agaaaactca
     2521 atcagagaat atcaaaaaat gtttaataat ccaaataata ttacaacaga tgaaggtggg
     2581 ttttcaaatt ttaaaccaga tatttattgg aaattcttct atccaaataa agctttttat
     2641 aattttataa tagagtcata tagtaatcca aatgattata acccagaaat aattaaatca
     2701 atgcccttag aagatggagt taatttttat aaatttagaa atgaaaaagt aaatacaata
     2761 ttaaatatgg aatatcctga taaaaattac acacagaaag agaaagattt ttggttatct
     2821 aaaaataagg atataaagga gccttattta aatggatatt atcaaggatg gaactatttt
     2881 ctattggcta gagatttact aatttttact atagtagctg tctgcataag tatagcttct
     2941 gtattttcag gagaatacca atccaatgct gactcaataa tattatcttc taaatatgga
     3001 aaaacaaaag tcataacagc taaaatagta agttcctttt tatttggtac gattattttt
     3061 acaataaata caattgtatc tattggcata cttcttttat cttttggaac tgatggctgg
     3121 aatttaccac tacaacttac aactagttca attccatacc catatacatt tttacaggca
     3181 atattactaa gtgtgttaac tatctatatg atattaatag tattaattag ttttacactt
     3241 ttattatcgt cttatatgaa atctcctttt ccagttttaa ttacaaatat aatattgtta
     3301 tttgtaccaa tatttttcaa atacagtgaa acaaataaat tgtacaatca gattctcaga
     3361 attttaccat caaatgctat ttcattttca tttactgatt atatagatta taaatttggt
     3421 aatttaatag taactttacc tacaatgatt atttttacat gcttggtgct tacagttgtt
     3481 tttttacctt ttgttaaaaa taattttaaa gaacatcaag ttaactaaaa tatatattat
     3541 aaaaagaagg tgtcttaaaa tgaatttttt agcttgctag gcactcttct ttttattaat
     3601 ttaaatatat tttcgtatta aattaaatat gttttcatct ttttaacaat gaaaaagagc
     3661 cttatctcta ttttgagata gctcttataa agttaaatat tgaattgata actataaaat
     3721 atgtagttta aatttataat ttatgttata aacaaactga tttttgtaca tcagttctta
     3781 cattaacatt acctgctttt agattttctc caatagtaag acctttttct tttagaacat
     3841 attcaaaagt cattagtgga actccacctt ttctaccacc taatatttct gttcctttta
     3901 aatcattgta agagaaatta ggcatttttt tcctagcaat taaaaaacta ccatctcttt
     3961 ttgttaattg agcaaagttc acggcataat cgtttttgtt ttgattatat acatatatag
     4021 atgcttcagg gcccattaaa cctacgtttg cttcacctga aatcaaagct gccatagttt
     4081 tatctgcccc ttgggtattt ataaggtcaa cctttatacc ttcatcttca aagaatcctt
     4141 tcgtaatagc agcatactgt ggagcataaa aaacagagtg agtaacttct gctatagtta
     4201 ctttatccag atttttagac gaagaagatt catctttttt gtcattagag ttaaaatcac
     4261 atccagttag acatattgta cttacaatga tagagcttgc tattgcgact atttttttaa
     4321 acataataaa tacctcccat aagacatacg aataatatat agtattaaat atttattgta
     4381 gttgttacaa aaatgtatct atgcttataa tatttctaca aaaacttaat aataattttc
     4441 agttttatgt ttagtttttt tagtaatgtg taatattgga taaattatat tgattattta
     4501 taaactaagt aataaaatta aaataatatc attttttgcc tattatatat aaggaaaaat
     4561 tttttaataa tataaatcat attcatttat attattaaaa aatttgttta tagaaacttt
     4621 tattatttca cgtaaaagtg ataaaagctt atattttgta tttaatagag tgttgctaag
     4681 tgaatatatt taaaatggag gaaattttat gaaacaaaat aaatatgatg atgataaatt
     4741 ttttaataaa tacagtaaaa tggaacgttc tacaaatgga ttagcaggtg ctggagaatg
     4801 gcatgcttta aaaaaaatgt tacctgattt caaaggaaaa agagttttag atttaggatg
     4861 tggatttgga tggcattgtc aatatgcagt tgagaatgga gcaatttctg ctgttggaat
     4921 tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa acgcaacttg ataatataaa
     4981 ttacatgtgt atgcctattg aagatattaa ttttcctaaa ggtagttttg atgtagttat
     5041 tagttctttg gcatttcatt atattcaatc atttgaagat atatgtaaga atataagtag
     5101 ttgtctttca aataaaggta attttgtatt ttctgttgaa catcctgtat ttactgcata
     5161 tggaagtcaa gagtggtact gtgataaaga tggcaataat ctacactggc cagtagatag
     5221 atattttgaa gaaggagtac gtaagtcaaa ttttctagga gaagaggtta taaaatatca
     5281 taaaacatta acaacttatt taaatacgtt aattaaaaca ggatttgaaa tattagaaat
     5341 tattgaacct caaccagaag aaaaactgtt agatacaatt cctggtatga aagatgagct
     5401 acgtagacct atgatgctac ttgtatctgc aaaaaaaaga taagcaaata taaataatca
     5461 ttattgtaga acgaaagttg tagaaaacaa ttacactaat agatatgatt ataccagtgg
     5521 tattaattac accagtagta aggaggcaaa agcattcatt gcttttgcca ttttttaagt
     5581 tgcaattata cttccacgta gtatatttca tttccatttt cttctataag ctttttcata
     5641 tcttcaaatt ttaagaatac agtagcagta ttatcatttg gatgtatacc tattttatcc
     5701 atttttaata aatccttatc aaatacaact tttacaacag cctctttatc atttaatata
     5761 ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt tttgaagtct ttcttctgat
     5821 gcaaaactaa gaggtttact atcaatttgt tgacgaatat cttttaaatc tgcttttttg
     5881 tctttaccta atacaactaa aaaatgttct tttcctttag catcacgaag aaataggttt
     5941 tttacaacaa attccatatg taatgttttt aattcttcca tttcttcaat tgtataaact
     6001 ggtggatgat ttacagcctc ataaggtatg cttaactcat ctaatttttt atatacttcc
     6061 tgctgttgat tgttcatgat ttcctcccat tagaaattta ttttatatat aaataatatt
     6121 atcacagaaa actttaaaag aaaacgtttt atataatata gacaatagat tatattttta
     6181 taaagaagca agaatactca ataaaactta aagaaattaa acaatttcta aaaatacctt
     6241 gaaaatagaa ccttatttgc catttataaa ttatataaaa agggattata taaagtcata
     6301 tatgaaaaaa ctatattata ctatcttttc tttttggaat aaactatact ttaaatatag
     6361 atatttaatt taaataaatt ttacagtatt aatagtaaat tatcttagaa aggaatgtgc
     6421 tttatatgtc tacagacaaa gttgttggaa tcatagatga agaaacggca gaacttgcag
     6481 gaattgaata tactggtaaa atttatgctt cttctggtgt tataaaacat ataaagaaaa
     6541 aacatagatg tcaactgagt aaggatattt ttaatgatat aatagatact ataaaaatgg
     6601 tattaaagtc acctgaatat ataggaagtc atcctaaaaa acctggaaaa agtgtggagt
     6661 ttataaaaaa aattgatgat tatattttag tagctacaga attagataca aaaaaaggat
     6721 atttatatgt atcttcaatg tatgctataa aagaagcaaa attagaaagt agagtaaaca
     6781 gtggaagagt aaaacagtat gatggttaac ataatattat taattttccc taaaatattg
     6841 aaaaatatta ttatataagt tataatatta atatacacgt agatatttat tagttagatt
     6901 tgaggttgga aaaggcaccc aatacgctac tatagagtag ttaatagaga tgcaggatac
     6961 gccgccctgc caaatctaat taaattaaaa agagaggatt aattcctctc ttattgtttt
     7021 ttgagatact aatttaatct atataaaaag attgaaggtc atttatacta aaagttttca
     7081 gttatataca ttaaactaat taaataagat atttataatc tatatgtgat acatattata
     7141 tattaataaa agaaaaatat atagaaaatc cttataaaaa tttaaattgt aatgaatagg
     7201 aagtaattaa aaagaatttt tattgatttt taaaaattta gttagaaact atatcattat
     7261 ggcaaaaaat gtattataat gtttagaaat attataataa ggagcgatta aacatgaatc
     7321 tatcgaagaa aatagaagat aaaagaaaag ctaaacagag agctgaaaaa gttaagaaag
     7381 ctaaaattgc tacagctggt gtggtattag gagctgttac tggtgcagtg agtggagtac
     7441 ttttggctcc taaatctgga aaagaaacaa gagaggatat aaaagatgct tcacaacaaa
     7501 ttgctgaaaa aataaatatg aaaactgtag atgtaaaagg aaaagtatct gagaagctag
     7561 aggataaaaa aggaaacttt atagaatcaa agaaaaaaat aaaaaaatat atagataaca
     7621 aaaagagtgt tgaaaattct agtgaggaag ataaaataat agatatagtt gaaccagtta
     7681 gtgttactga ggaataatat ggacaataca ataacgatta acataagtat aatatatgta
     7741 ttattagcta tacttgcttg tattgcttta gtttatttaa ttattgtact tgttaaacta
     7801 cataagattt taaataatgt tggaactatg ttaaatgaaa atactcaaaa tataaataca
     7861 actttaaata atcttcctaa tgttgtagaa acttttggag aagtaggaga aaatatgaaa
     7921 gatgtaacag aagtagttac tgaagttaca gcagattttc ttgttactaa agacagtgtc
     7981 aaatcaaata tggatgttat agttgatata ttgactataa ttaaaaatat ttttctagat
     8041 aaaaaataat aagtttaata taaaataaga tataatttaa acgaccttaa aatagcattt
     8101 ataaggtcgt tttttattag cttaatttga cactatgtgt ttttaataat tggtaatatt
     8161 tctctttatc atttgaacat atatatctat gcttgttgtt tcaattacat ccataagaac
     8221 atctttacat tcaagctcaa aatcttccat tccttctaaa tattcatcct ctgcatcaaa
     8281 aaagatataa gaattatata gccattcttc aaatttttct tcaagtacat cccagtacga
     8341 actccaaaat aatccacaac ctgacatata agatgctcta ctttgattta tataagtctc
     8401 ctttagaagc ttttgtaatt tactgctata aagaaaatct gcttttccac tgcttgatat
     8461 taattcattt aaatcttcta ttgacattaa ttcaacttta ccacttttat cataaggagt
     8521 ataattttca tcaacccatt tatgtggttt gttttctctg catactgttg ctatcaactc
     8581 tacaactttt gttcttatca tttttttatc ctaacttatt tattttttat aaaataatca
     8641 taattttata atttatctat ttcttgtaat gctaatttaa cagtttcata tctatcagca
     8701 ccatttatta ctacatcacc ttttacaagt ttattagctt ctccacctac aacataaacc
     8761 tttttaccta aaccttgctt ataactagac gcctcaatta ttaaagaatc tttcaattgc
     8821 caatgtaaaa tctgagcagc cactttatca gcatcacctg tataaacgat tacattttca
     8881 tacactttat tttcccctcc cccttgagaa tttgatatag atttgtttag tataccttct
     8941 gctatcaatt tagcaacttt atctttatta ttaacataat aatctgtatc tgctttacta
     9001 tctacaaaac atacttctat taatattgct ggtgcaatag tatgattaag ccaataaagg
     9061 cctctaacat cagactttgc acccctattt ttgaatactg tagctaacct agtattcact
     9121 ctttctgcat aggttttgcc attattagtc ttatatattg tttctgtccc tactggagtt
     9181 gaagttgtga catttgaatt aaaatgaatc tgaatagcca aatcagtttc ctgtttattt
     9241 gctatagcac attggtctgc taaatgatta cttgattcat caactcttcc tgtataaaca
     9301 gtagctccac ctattttaag ccatttaaca attaagtcat tcagtattct tgtttctttg
     9361 ctttcgttta tatacccaac agctcctgta ccttttccaa ttaaagtgtg ccctggtatt
     9421 attactactt tcaaactaac atctccttta caattaaatt taacttctat ttaatatatg
     9481 cataacctag aattttgttt ctttttatat cttatacttt tattataacc cattttgtaa
     9541 aaatatagaa tattaagtaa ataatgttaa aattataatt ttgtatataa aacattactt
     9601 gattgcaata attttatagc atattcatct attctatatc cagttctata atatatatgt
     9661 ttaattccag cttgaattat agccttagta caatttaagc aaggaaagtg agttacataa
     9721 atagagcaat ctttaactga aataccttct tttgcacaat aataaagggc attttgttca
     9781 gcgtgaatgg ttctaataca atgtccatct ctcatttcat gaccaacttc actacaatgc
     9841 ttatctccag aaatactacc attatatcca gtagatacta ttctgttttc tgaattgaca
     9901 attatagctc ctacgtaagc tctatcacaa gttcctcttt cagctactgt ttcgcacagt
     9961 ctcataaaat attcttgcca tgagcatcta ttttcaagtt ttttattcaa atcatatacc
    10021 tccaaaattt atatctaata tataggattt ataccttaaa aataatatta aaaacaaatt
    10081 tatacgataa ataaaattta ttaatactgt ttttattcta aattatttta ctttaaatta
    10141 aagttacaag gaatctaaaa ttatatatat ctttctttgt aatatgaatg ttataatata
    10201 aaaaaactgt ctcaataaaa tagagacagt tttacagtca aagtttataa tacaagttca
    10261 tatttttctt taaattcttt tacagcttcg ggccatgcct tttgttctgt aatacctttt
    10321 tctttagcta ttttaattgc aatctctctt atcattgcag cgccttctaa tattcccatt
    10381 tttaatctcc cttcaaatat ctttttgtat agaatataaa tagtgctaag atttattaca
    10441 tgctgcatat acaatatcta tacaacacat aacttataaa ttccttttat tttcatacgt
    10501 ctttaaccga catataatta tattcaataa gttttaaaat tatgaactca attataatta
    10561 tttttatttt tttagaatct aaacactaaa aattgtaggt aaatttgtct tatgccttta
    10621 taaatatttc tacagtttta attattgtac atatctattt ttattataaa aaatgcttaa
    10681 aaatcgagaa catacgcata aacactgtat ataagcaaaa cggttaaagg aaaaagaaga
    10741 aatttacaga atggttcgaa aagcccactc ctttaggggt gggatggata gcataaatag
    10801 tgtataaaaa gtattgatac atacacatat aatgttaata taagtatatg gagaataaat
    10861 atagaaaaac tcaaacaaat gtcagtttaa ttaattatca ctttgtattc tgtcccagat
    10921 ataaaagaaa aatatttaat atagaaggct tagaagatag ggtattcaaa gagattgcta
    10981 ttctgcattt tttaataatg aatgttaatg atgatttaaa gtcaatcaac agagaattgt
    11041 gtgttaaaac atatgataac tttaaaatat tacatgataa agaaatagat aggctaaaag
    11101 aattaaaatt aaatggttat aaattaatat ctagtatggg gatataaaaa ctctatatag
    11161 attgggaaac gagcctaata ttaatgttaa tggtgtcaac agatactttg ttaatagaag
    11221 tcttagggaa tatgattagt gataatatat tgtaagttat gatttattat aactgagagt
    11281 attgtagaaa ttcatgtagt atataaaaac cctacacctt taagtgtggg agtttcagtt
    11341 gaatgttaaa atgcgttgct tgaagcaacg ggtagttttg cctataatag cgttaacaac
    11401 tgaaagaaag gtggttatcc ctaatgttaa gcgaaaacat taaaacaatc aggaagtcaa
    11461 aaggactttc gcaacaagaa cttgctataa aattgaatgt agtacgacaa acaatatcta
    11521 aatgggagca aggattgtcg gttcctgatt ctgatatgtt gatttccata tcagaggtac
    11581 ttgaaacgcc ggtaagcact ttgcttggag aaactattgt tgagtcggaa gttgataact
    11641 taaaagtgat ttctgaaaaa ttagagatta taaacttgca acttgcacaa agaaaaaaca
    11701 tgagaagaaa aatgtttcat tggttattta ttttattatg tgcagtcata gtaataggtt
    11761 ttttggtctt agggggacta aatagtcctt atttggattg gaattatact gaccctgaaa
    11821 ccgctgtttt tggagtagct tttcatgcgt ttgagtggct atttgtcaga ttagcaccga
    11881 ttgtccttat aggtgcaatt attggaagct ttttgacacg taagaaaata tgaagacatt
    11941 ctgcttttta taatacagca agcgttactt aaaagcgtca ttctacatga catcgaggac
    12001 gattatgtaa ggaagaatct ttttgatttc caactaagcg aagtcattga ggacgacaac
    12061 aagcccaaaa ccgtattaag tgaagtacag gaagcaagac tgctagactt atacgcccat
    12121 gcgtcagagg caaagaaatg ctaagtctga tagcatgatt tacaacgatt tgtacaacga
    12181 ttgcgagtag aaatatatta taaaattaga aaagaaatga cttgataatt tatccatgtt
    12241 ataattattg atgtgaaatt aataaatcga aattcctttt ttagatttaa caatactgag
    12301 taaggaaaaa gtgaatgact tttgcaagga ggtgttaata ttagacaagt aaagtctttt
    12361 gtttttccag ttatatcctt aatattctta gaccaaatta gcaaagttct tataggatta
    12421 ttcttaatag actttgaaat tgatataatt gggaaatttt taagattcaa tcctgttcaa
    12481 aatacaaatc tctcttatgg gggaaacttt attggtattc tatctaattt atgggtattg
    12541 gtattgttta acattttagt tatcctagtt attatatctg gatatgcttt ttacaaatca
    12601 aaaaatgaac aaacgagcta ttcagtaaaa gtaattatgt cttgtggact tgctggtaca
    12661 atatgtagct tgatagataa attattttgg ggaggaagtt tagatttttt gcagatacca
    12721 agctttttta ttttcgactt aaaagactgc tatcttactg ttgcagaaat aatatttgtt
    12781 atcataggaa ttttacatag tagagaaata tcaatgaaag aatacatata tttttgctat
    12841 cgtcagttta aaagataatt ttagaagata aatttaagtt tcaaggtaaa ctatttaatc
    12901 acagcaaata gttggctcac ttcagctttt gggctaaact gtgagcactc attaggtctc
    12961 tgctatctaa caaacggagc acaaaacgga gtacaaaaat gaaaattaaa tattgaatat
    13021 aaaacacagt taattttgat taaaaagttg gctgtttttt cttttgtaga aatttaagca
    13081 taggtaataa aaaaataact gtatttatgt ataattggag gcaataaaaa gccagtaaaa
    13141 cccataaaat agggtcatac tggcaacatg gtaagtttat aagaagataa tcaaaaattt
    13201 acgctttatt ttaatttaaa aaaatgatta ctttaaagaa atcatttttt attataatca
    13261 ctgagacatc aaatattcta aataaaattt ttacttatct ggaattgtat attttttcta
    13321 taatattaac tacagcgtac attgcccatg cacagatagc caatacaaat actcccatca
    13381 ttactaaatc taatttaaat acttgagaac catatactat caagtagcct attccatatc
    13441 ttgaaactaa aaattctcca actattacac ctacccatgc cataccaata ttaattttag
    13501 ttaaatttat taaatttcct gtgtttgcag gtaatatgag tttaaataat atttgagatt
    13561 tactagctcc aaaactcttt aacatcttaa ttttttcttc atctatattc ataaaataat
    13621 tataagctga aagtatagta acaacaacag aaatagttac agctgttacg acgattcctt
    13681 ctattccagc acctacccaa actataatta taggagctaa agcagtctta ggaagagcat
    13741 ttaaaactac caaaaatggg tctaatattt ttgataattt ttctgaccac caaagagcaa
    13801 ttgctaccaa aatacctaat acagtaccta taaccagacc aagagcagtt tcatatacag
    13861 atattccaat atgtttaaat aattgaccac taaatgcata ttgaataaat aaattgtaaa
    13921 tatcacttgg tttgctaaat agaaatgtat ttataacatt taagtcagct aaaagttccc
    13981 agattacaat aaaaccaatt aaaattaata gctggtagaa taatacattt cttttttctc
    14041 tttttacacc tttaacatag tttatatatc cttcagatct tttattaagc ctcatgtgta
    14101 tctagctcct tccataatat gtcgaaatac ttttgaaaat caggaacttt acgtgttttg
    14161 agaggcgttt ttgttccatc catgttaagt tcaatatcgt aaatattttt tatggtagct
    14221 ggtcttttag atagtattgc aaccttatct gatatgctta ttgcttcacc aatatcatgt
    14281 gtaaccaaaa tagtagactt attctcattc ttaataatac tataaacatc atcacaaact
    14341 aataatctag tctgataatc aagagctgaa aaaggttcat ctaaaagtag aatatctgga
    14401 tttacagaaa gtgttctaat aagcgcaact ctttgtctca tacctccaga taattctttt
    14461 ggatacatac ttctaaaatc ccataatcca taagtcttta aaaggctttc aactctatct
    14521 aaagattttt tatctttttt gccttgaatt tccaatccaa tagttatatt atccattata
    14581 tttctccatt caagcaaatt atctttttga aacatatatc caatatttcc agtaatcttt
    14641 atatcaccac tagtcggttt taaaagatta gtaagaatat ttaatattgt agatttacca
    14701 cttccagaag gaccaagcaa agttaatatt tctccttctt tcaagttaaa attgacatct
    14761 tttaatactt caagctcgcc ttccttagtg tagaagttca tatttatatt tttaacttct
    14821 actatgttgt cattcattat gccaccccct ataactttta gctcgtatat attatgttat
    14881 taacaatatg acctatttgt accatttttc ataaatataa tataaatatt ttgtttattt
    14941 acgtattttc ttagtaactt gtacgtataa attatagata aataaattat aaggtagggg
    15001 ataaatttga gtaacataat aacgaattta atagagtcaa tatttactaa tccaataact
    15061 atattgtttt tacttgcaat aactgtgtat tctgttttta aaataataga aaataacaga
    15121 gtatattcat ttgtaagttc aaaattagac gaaatcaata gagactataa gacaagtgag
    15181 ttttataaaa aggtaaggga tgactactat acatatagta aagaaaatcc ttatgcagac
    15241 gtaaatataa cttcatttat agaggaagtt gtatctgatt taaagcataa taatttgcca
    15301 ttgttagaaa aaattagaag cataaaaaat tcatcttcaa taagtatatt acttggggtt
    15361 ttaggtacgt ttgttggtct ttctacaatg cttttatgtg ttgatactaa agacattata
    15421 aattcacttc cttcaactat tagtagtatg caaactgcat ttacaactag tatatttgga
    15481 gttgtatttt ctctaattat aggttatttt actaagataa aagattgtga acatgttttg
    15541 atacaaataa tgttaaaatc tgaaaattta ctaacatctg aaataactca ttttaagtca
    15601 gaaagaatgg atttaaaagt agaagaggta aaaaatacaa taaaacaaat aagtaagtct
    15661 atagaagcta ttgaaaaatt tgataaaata tctaaagatt taaatgactt taatgatgag
    15721 tttatcagcg gtatagaagc tttaaaaagc cttttagagg gttctcaaag ttctattaag
    15781 acatttgacc agagcgtaag aaaattagat aaacagttta atattttaaa tataaaattt
    15841 gtaaagcttt ttgataaata tgataatcaa gataatataa ataaagagat tttattcgat
    15901 ataaaagaat cttcaaaaaa tatatacaat gccactgaaa gccaattcaa aattagagat
    15961 tatataaaaa atataaatgc aggatttgct ttatatgaaa gaagtgctca agatttatta
    16021 actaaattaa tgacccatga aaataaaata tctcaaaatc aaaaaatcct tttagatgaa
    16081 aagtttacct tagatgactc aataaaaaat ctctcaagta ttattgaaaa cttttctaat
    16141 gacctacaag ttaaactgga tatgatgttt gaaaattctt tagatattca agataagtta
    16201 gatgttatgt ttaataactc ttttatgaat gatgaagttc ctcttgacag tgaagaatta
    16261 tttaaggatg atgttaataa tgtatttaat ccttttagtg aagaaattta tgagatagag
    16321 gataaagaaa taaaggtcat aggtgaggat gagttaaatg aataataaat atagaagaac
    16381 tgttaacaga gaatttgaaa agagtagctt ttggccaaca tttacagatt tattatctac
    16441 tgtattgatg gttgtgattc ttatcttatt tagctcagaa agtatttcag gttctgttga
    16501 acaagattta gctaagaatg tcaatgcttc tgttgaagaa acttttaaaa aaagcggaat
    16561 acctgtgaag gtagataaat ctaatgggca agtaactttt ggtgaaagaa ccatgtttga
    16621 tgtagatagt gatgttttaa aaccagaggc taaagaaatg ctaaaaatgt ttgttccaaa
    16681 gtacatagag actatttata aagattatgg tgactatata tccaaaatag ttatagaagg
    16741 tcatacagat gatgttggaa gttatatata caacttagat ttatcgcaaa gaagagcata
    16801 tagcgttgct aaatttatag tgggagatga aataggtgac tataaataca aagataaagt
    16861 gactaagcat attattgcaa taggaaggtc aaaagctgaa ctcatcaaaa atagtgacaa
    16921 tagcgtgaat agagatgcat ctagaagagt agaactaaaa tacgaaataa atattaatca
    16981 aaataaataa ttaaattttt ataaaaaaaa agaaggcagt tggtgtgagc tttaagcctt
    17041 ctttaaggaa aaagtttatt tatttttgtt gatacttaat atattatagt ttttagaagc
    17101 agtttgcaat atattaaacg taaatggtcg tttaaaatgc ataactggat actactgatg
    17161 taaaatattt cattttatat aatataaaaa t
//
LOCUS       DAEMAE010000066        17060 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEMAE010000066 DAEMAE010000000
VERSION     DAEMAE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.067"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            40..636
                     /locus_tag="KPH93_001750"
     CDS             40..636
                     /locus_tag="KPH93_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688575.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIEDKTVYMIEKIFISIED
                     EEIKNYLIRNNCKLQELVNRYKKLELLNIDEAIFFAWYNLNINTISIDKASQYYYELT
                     TSNYIEVESHLVYTNEIDLREYAKDELDTMLDMEYHIDRLLDKDMIIDMWLNNTSKED
                     VIEEILMSDDIEDILDISPEYAFTTTSGIEYRYSEKEE"
     gene            832..1047
                     /locus_tag="KPH93_001751"
     CDS             832..1047
                     /locus_tag="KPH93_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBG1688576.1"
                     /translation="MVDILGEEIVIKLHKYYRGQQITFPMKLYSNEYVERYIEKNYRT
                     KTLKDMCRELGYTEGWIKQLINKYKLK"
     gene            1276..3468
                     /locus_tag="KPH93_001752"
     CDS             1276..3468
                     /locus_tag="KPH93_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688577.1"
                     /translation="MTKFNFIGNEIYITEERNRYNSIRIEYENIANKAREEFIKVYRS
                     CNENLYDVINNAYDQGASIILKSIKCTLDRLIENKFYNISEELFIEQYCQRVVEIWES
                     AYGIINDQYMNIVLDERQKEEYRQLRKNSRARWQGGGFGIQGAIKGATQAGAMNMATG
                     VAHSAFNMISKIGTSIKVNKQKSKIFNDPSTLDVLSEVIYLAILDIKYSYIKFLENNA
                     GLQFGYIHEEEEEEANVILSNIKGRNLDEEEKINLIKGLIDLNPYMESLYTYIVDNFG
                     DENMEVSKMASYFGFNIEPYKLSLVENKLINLETNTEEETILAKQLILKEVNRLGINS
                     KVNGIEELDRKLNQFDIEARTVDNILFETREDANLARIEKEKIDLILSKINMKIEEEV
                     IRLKNDILSLKLKTGIEDSYIKMIDEKLQKFDIEARTVENLLLDTREEAEKIKLDKIK
                     VEEIINNIDETSEQSLLNAKTEIENIGIRLESTKGAIEKLNYSLKKVDEIERTVNEIL
                     FDTREEANVAKKEMLELDEILQNVDLDDEESIKVAMSKIKSHGFKTKIGDNEANKLNK
                     KLEDIDTQSKMVGDILFETREKANLARQEKLDIESIIKNLDENNEENLVSIKKEIESR
                     NFKTEIANLYIDRINNHIKNLYSDTINEAEQYEDNKTNFKSMLIGSAFIVPLGIYFFG
                     NVGIILKIVIGIFLLSAVSALFESYKKLKASKCSLKQLKKLKKSGKII"
     gene            3762..4100
                     /locus_tag="KPH93_001753"
     CDS             3762..4100
                     /locus_tag="KPH93_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688578.1"
                     /translation="MEQYSFSSELIDVMVNASNDLNRVYEKITSSGTFSGLQGTFDTL
                     YEYGLMLEDLLEVMDLSLLGGNETKEIIQNIAIDLKNISDEDNIADKALDLMGNIGEF
                     TYYIETNYSK"
     gene            4235..4480
                     /locus_tag="KPH93_001754"
     CDS             4235..4480
                     /locus_tag="KPH93_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688579.1"
                     /translation="MRSVTVEDGEERFAIEKIYVKSLKRYEVRICLYRDCKDRMNKLI
                     PRPVDISPDKLSTLILVGIKAGILDDDFKQELIKGLS"
     gene            4778..4969
                     /locus_tag="KPH93_001755"
     CDS             4778..4969
                     /locus_tag="KPH93_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688580.1"
                     /translation="MKYTLKYGLGQLKFDVKIRLEDILRLSSAGEDNDEVRLAERSRE
                     INDIIGKLYLRPSETKKKI"
     gene            5058..5969
                     /locus_tag="KPH93_001756"
     CDS             5058..5969
                     /locus_tag="KPH93_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688581.1"
                     /translation="MEKYVFSFESGNNKLFKEYWKDRKKTDIPLITTIFFRTMLRLKD
                     TYRHDLYNLWEDCNLTTEEFKENIYKSQNESIRFIKFKVEKMIEEEKERLGKSINNKY
                     KSNRKITELQNIDIDNIVKELIISDNPENKEIYQKMIIITKSFFKIIEYRRKLSLSNQ
                     TATDNLMEEVKKLYNTLPKESRYDAAYKNLRKLKEDIEFKIKEIDNYKEIYKREVIKN
                     CKLVSEYKKKEEKLKKSGYIEESEKMAKKYIDIERQFNESMRKKWIYIDMYIRRKDLE
                     IEEMDMKKYEDDNITNSMIKLAEEINS"
     gene            6149..6430
                     /locus_tag="KPH93_001757"
     CDS             6149..6430
                     /locus_tag="KPH93_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688582.1"
                     /translation="MELKTIRITFTVSSETSEDDLRKLCKDYMGDEYVVNDEGDFEIS
                     FYLNTVLGYNEVLYFMELIHMELEKSITLLGIRYKGIGGLQMKHILQEE"
     gene            6437..7567
                     /locus_tag="KPH93_001758"
     CDS             6437..7567
                     /locus_tag="KPH93_001758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688583.1"
                     /translation="MIRNCSKIIKNYGFKINSKNHVFDSNGNEICNFVPIVLGQYMIG
                     DGRDRRIVIVLMGISEDGNQLETINIDVSDIEHLKWVKNSWGFQYKVHSKMKNQFLYL
                     IEVLVINGTTKKLSSQIGWVKDNGKYAYLHTRGVIGESDKEVDIGDHLSNYFLPSEIV
                     DIDRACKASLNLLEVADYEVTVPLLALVYLSPLLQAIKDVGKMPEFVVWLFGTTCSRK
                     TSLARVFLSHFGDFTNRLPATFNDTYASIEIKANAIKDSLNLIDDYAPKQTKKQKDAQ
                     DEIAEKAIRAYGDRIARGRANSSMISQKQYIPQGMLLMTGENLIRGHSTVARLVALEL
                     DKDSVNLKLLKEMQDSVSLLGESMRGYIEWLLHGMNKDSSDI"
     gene            7694..8191
                     /locus_tag="KPH93_001759"
     CDS             7694..8191
                     /locus_tag="KPH93_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688584.1"
                     /translation="MLSYFEDKKVISEVEFNQYIQFAKDTMVNVLIKNNNITKESSPI
                     DEFLYTIKEAIDSNSIKISTLVDGNKLNDNDDDTYGYKDDKYFYFHPDKTYSYVQEIQ
                     SKSGNYISLTKRGLIKLLREHSIIKVDSDGSPSKYTIKVKNGTDYKEKRPRLLRVAIN
                     TIEKL"
     gene            8233..8676
                     /locus_tag="KPH93_001760"
     CDS             8233..8676
                     /locus_tag="KPH93_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688585.1"
                     /translation="MTIRINLNGENILENADNKFYGLRARVEFDLDLLDEDEDVIDIM
                     DRILTLDVKPSRMFITKGRVDIFYQPQKEIAIQSKSGYMKLINEFIDFIESQEIVEMK
                     FSGWNLNGDKVVESQANDSNISFSKEIFNPDTVYLDGLIEGYVNL"
     gene            8783..9700
                     /locus_tag="KPH93_001761"
     CDS             8783..9700
                     /locus_tag="KPH93_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688586.1"
                     /translation="MNYITTQGFDTIKKDKIEIKRMVYALENEGDSYNVIEECIYTLE
                     ENIDVNDDFVIEVENVFSFVINRIMDFTKERNLPKNKIEHVRAYIDVFENPTSNTKIV
                     KSIDIYEDYDKRYIDNELDKLFKSMDNMIKPEFVPVIDGKFDIRGSKDLKEYNKKLML
                     DIVKQKTYEVNNGGIRINKLSAILKLGDKSIDGISKMMKDVANLKIKPKSVYISEDTV
                     VVMYICNKSCIVLNIEEYSKILSDFVDYINNIEEMDLYLDDSILYGDYKINCGVDDRD
                     IIDFNYQSFNSKVTLFAGHIGNKIIQAAL"
     gene            9730..9885
                     /locus_tag="KPH93_001762"
     CDS             9730..9885
                     /locus_tag="KPH93_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688587.1"
                     /translation="MTETYKISIGRKDLYSKDYKAVERIDYEKRYIVNISRSLVDILS
                     ILIEEGI"
     gene            9967..10944
                     /locus_tag="KPH93_001763"
     CDS             9967..10944
                     /locus_tag="KPH93_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1688588.1"
                     /translation="MKSKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQL
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNDLGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEDEIIKVEGGMPVIIDKEVFEQAQ
                     EMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNICFI"
     gene            10922..11530
                     /locus_tag="KPH93_001764"
     CDS             10922..11530
                     /locus_tag="KPH93_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1688589.1"
                     /translation="MVIYVSYRCGCRKQKRDCNNREIKRDYLEEFILQELEKNILNDE
                     AIPALSKALNEKLNYKNESNAELLKNLDQKLEKVNKEISNILNTIMNGIVSSMLKDKL
                     DELEQVKLNLDLKMNELKIENKVVDGVGVTEEQIRSMFSRFKEFVLERNIPECKKFIG
                     DYIKEVIVYKDHVEVVFNVVFSFVDDEIAHNLHITVSRVKVK"
     gene            11663..12727
                     /locus_tag="KPH93_001765"
     CDS             11663..12727
                     /locus_tag="KPH93_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBG1688590.1"
                     /translation="MSKIEYYMNLPYDLSGSMSKNRFRNELLWGLKKMYELYKTNEDF
                     VVVFDYRCDIEVHLENKFEFYQVKTQNNSGTYTINKLTDKKKGSKSVVGNLYSLKYDI
                     NMNEIDDVYVAIVSNAPLNDGKKTYNNSHEVKIKTIDSSAIEKIKLSIKNDLNIDEDI
                     NLENLFFIRIGLDLIEPQKTLIGETAIFFEDVFGRECSKVKSLYNVLYEEIYSKSAYE
                     LDINTYENIVSKKGINRDRMDIILKSYANNTDKSVELAKYSINEWYKNNFSRRLSLIK
                     SLTTIVSKLSESSILKDIENCILSYIYNNIDKLQVSDKELIEIISEIVEEKRPIEVSK
                     TDIEALILLTLKKYEEGVYE"
     gene            12720..14393
                     /locus_tag="KPH93_001766"
     CDS             12720..14393
                     /locus_tag="KPH93_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688591.1"
                     /translation="MNSLFVNSLYIFEYESKMAKKIDFKHGINILTSDEKSGNDVGKS
                     VLLKSIYHTLGADSIFDHMWNKITKTYILNVTIKNKTYYVYRRDNLFKIYNTNFKKIF
                     MTNNRDELSNYLSDLYSFKVMLPKRENEELELAPPVYSYLLNYIDQDHLDGSKFSSFN
                     SLTQFPNYKENVIYTHFGLFDEEYYKAIKELEILKSEEKNKKKEREVTLNMLNKIKSY
                     LGNLDAPESIEVLNIELNKTKEEYSNIVINLQKIKNNLMKIRNEKVDLELNIKEIELT
                     RRNEMKKLKVKNVCPSCSHEIEDIDSKFIINNQIEDLIILKDELESEIIKCNRVIEKK
                     EEEYQSLLDKLHEYESNMKINDDSISNVLKHKAYIDTKDDIVLDLEIIDKKIKEIQQT
                     IEPYDKKIKEYNSLKKKANAKYEELMKKAKKDFGLEEIDENKIKNIKNNFIARGSNRA
                     ITTIMWYLTLLAVKYEFNENAIKFPLILDSPNNVESDEIKEESLFKYIFKNTKTDTQL
                     ILSSLGFNEMQFSEFKFDNIIKLENSKYNLLNKEDYYNNIDILNKIFEN"
     gene            14498..15460
                     /locus_tag="KPH93_001767"
     CDS             14498..15460
                     /locus_tag="KPH93_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688592.1"
                     /translation="MKDRFNLKRKSIQSLMNKNVDVYSCKDCIDDFIELNFKIYRSYY
                     ISDDDILEIMDGTGEDYKDVIADMLPDKAHIKSGEFGEITATEIYKSNIQTTEIYIVD
                     KLSHTRKEDTKVAAHKTDILIIEKTSEGFIIHSSEVKTKITDSDFNPIEAMIEGVNDD
                     IVTRVAETLNWIQRKLKTEKKFDELKFIKQVIESIENQNVENEYNGAVFIDESKLDYE
                     INKNIYCDVKLSKSQKGKYDNELKSIGCVIDGDKIRFDSVNIDDINKLNINSRKRNSI
                     EKLYNKSQRKLEHIDDIKIQIVTFDDINKHCLDMYEKIIHVGRI"
     gene            15460..>17060
                     /locus_tag="KPH93_001768"
     CDS             15460..>17060
                     /locus_tag="KPH93_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688593.1"
                     /translation="MSINNNINKIRNLDIQLAKYDCISRMSAKETSKHYMRLYFKFIK
                     LLELYEDSSLNINNGEYLNLANDLIIFSKYDMSSRFKGIDEERNLVYIAVIYYILGYR
                     FECNLILDILDMHDIENEYLKIIIYLIRNKYYKCDVLSCIKKYYIEDESNLSLIIKTL
                     YEKELVCDTKDQTYIQIIIAILRNIQNNGFYRVISKKSKLDINIWRKFVQQSIKYDQI
                     IYDLPKYHYDLLNDELLNIKHGYINMPTNYSKIEVIKIVIFNYLHTYQDKKIAYIHVD
                     KDYRAINAIKEYFTEYSTRIEFIEIDSINNYSEIATDNNFIIIDDFSNVSNKYYGTRF
                     EIMISLALKLEKRLLVLNNNYLKMDSNQLENINYNEDINRYSNIVNYYFKDGKNKELE
                     IEYEKERFVTESYLLNKEINNKINLKGIKLNSAGDKSLLSAINLISKGYKVSIFIPQK
                     NSAKSGINKICIKLLNLIDRLNGKILYDKNCIDDLNLLDRAELLLGRESIYMKLFRLG
                     VIVIHSDMPQEVIEYMRYFIQCGKY"
BASE COUNT         7603 a         1363 c         2985 g         5109 t
ORIGIN      
        1 aaatgaatat taactaataa ttaaaggaga acgagtaata tgataaacaa taaatctgaa
       61 ataattaata agctattttt caatgaatta aaagaattaa tagataaata caacaacata
      121 gaagataaaa ctgtatatat gatagaaaag atattcataa gtatagaaga tgaagaaata
      181 aagaattacc taataagaaa taattgtaag ttacaagagt tagtaaatag atacaaaaaa
      241 cttgaacttt taaacataga tgaagctata ttctttgctt ggtacaactt aaatataaat
      301 acaatatcta tagataaagc aagtcaatat tactatgagt taacgacaag taactatata
      361 gaagtagaaa gtcatttagt atatacaaat gagatagatt taagagaata tgctaaagat
      421 gaattagata ctatgttaga tatggaatat cacatagata gattattaga taaagatatg
      481 ataatagata tgtggttaaa taatacatca aaagaagatg tgatagaaga aatattaatg
      541 agtgatgata ttgaagatat attggatata tctccagaat atgcattcac aacaactagt
      601 ggaatagagt atagatattc agaaaaagaa gaataaaata aatatgtgtg ttgaaaaaat
      661 acgggcttta agggcttata ccttaaagta tgtataaaaa atacaataga ttttacaata
      721 gaaaatcaga atgaaacttt ttctaatact aattattatt aatgagtatc agaaaaggag
      781 gtggagtaat gatcaagaat aatagcgact atcaaaggga tatatgcaga tatggtagat
      841 attctaggtg aagaaatagt cattaaatta cataagtatt atcgtggaca acaaataaca
      901 tttcctatga agttatactc taatgaatat gtagaaagat atatagaaaa gaattataga
      961 acaaaaacat taaaagatat gtgtagagag ttaggatata cggaaggttg gataaagcaa
     1021 ttgataaata aatacaaatt aaaataaata ccctagttat tgttaaatta ttcaataaaa
     1081 aatatgattt tattacaaaa atttacaatt tcgacaagat gaatgttgta ttatatcaac
     1141 gaaagatatt aaatttattc atatcaaata taaattatca aactttataa ctaagttatc
     1201 taataagaga cattagtaca aagttcatta gtaagtgaac ataaagaatt aaggcaatgg
     1261 agagggagat tgaaaatgac aaagtttaat tttataggaa atgaaattta tataacagaa
     1321 gaaagaaata ggtataatag cataagaata gaatatgaaa atatagcgaa taaagcaaga
     1381 gaagaattta ttaaagtata taggtcatgt aatgaaaatc tatatgatgt aattaataat
     1441 gcttatgatc aaggggcatc aattatttta aaatcaatta agtgtacact agatcgactt
     1501 atagaaaata aattttataa tataagcgaa gagttattta ttgaacaata ctgccaaaga
     1561 gttgtagaaa tttgggaatc tgcatacggt ataatcaatg accagtatat gaatattgta
     1621 ttagatgaaa ggcaaaagga agagtataga caactaagga aaaattcaag agctagatgg
     1681 caaggtggag gttttgggat tcaaggggct ataaaaggag ctacacaagc aggagcaatg
     1741 aatatggcta caggagttgc acattctgca tttaatatga taagtaaaat aggtacgagt
     1801 ataaaagtaa ataaacaaaa atctaaaata tttaatgatc catcgacatt agatgtatta
     1861 tctgaagtaa tttatttagc cattttagat attaaatact catatataaa gtttttagaa
     1921 aataatgcag gattacagtt tgggtatata catgaagaag aggaagaaga agcaaatgtg
     1981 atactttcaa atataaaagg cagaaattta gatgaagaag aaaaaataaa tttaattaag
     2041 ggacttatag atttaaatcc atacatggag tctctataca catacatagt agataacttt
     2101 ggtgatgaaa atatggaagt ttctaagatg gctagttatt ttggttttaa tatagagcca
     2161 tacaagctta gtttagtaga gaataaatta attaatttag aaactaacac agaagaagaa
     2221 acaatattag ctaagcagct tatattaaaa gaagtaaata gattaggtat taatagtaag
     2281 gtaaatggaa ttgaagagtt agaccgaaaa ttaaatcaat ttgatataga agcgaggaca
     2341 gtagataaca tattgtttga aacaagagaa gacgctaatt tggcgagaat tgaaaaagaa
     2401 aagattgatt taattttatc taaaataaat atgaagatag aagaagaagt aataaggtta
     2461 aaaaatgata tactaagctt aaagttaaaa acaggaatag aagactcata tataaaaatg
     2521 atagatgaaa agctacaaaa atttgatata gaggctagaa ctgtagagaa tttactactt
     2581 gatacaagag aagaagctga aaaaattaaa ctagataaaa taaaggttga agaaataata
     2641 aataatatag atgaaactag cgaacaaagt ttattaaatg caaaaactga aatagagaat
     2701 ataggtatta ggttagagtc aaccaagggt gcaattgaaa aactaaatta ttccttaaaa
     2761 aaagtagatg agatagaaag aacggtaaat gagatactat ttgataccag agaagaggca
     2821 aatgtagcta agaaggaaat gttggaatta gatgaaatac tacagaatgt agatttagat
     2881 gatgaagaga gtataaaggt agctatgtca aaaataaagt cacatggatt taaaacaaaa
     2941 ataggagata atgaagctaa taagctaaat aaaaagttag aagatataga tacacaatct
     3001 aaaatggttg gagatatatt atttgaaaca agagaaaaag ctaatttagc aagacaagaa
     3061 aaattagata tagaaagtat aataaaaaat ttagatgaaa acaatgaaga aaatctagtt
     3121 agtattaaaa aagagattga atctagaaat tttaaaactg aaatagccaa tttatatata
     3181 gatagaataa ataatcatat taaaaattta tattctgata ctataaatga agcagaacaa
     3241 tatgaggata ataaaactaa ttttaaaagt atgttaatag gatcagcatt tattgtacct
     3301 ttaggtatat acttttttgg aaatgttggg attatattaa agatagttat aggtatattc
     3361 ttattatccg cagtctctgc attatttgaa tcatataaaa aactaaaggc aagtaagtgt
     3421 tcattaaaac aacttaaaaa gttaaaaaag tcaggtaaaa taatataatt atcatgagga
     3481 atcaaattga aaggtatatt taataaaaca ttgacaaaga ttataataag ttcattaata
     3541 tttggaactg tatgtagtag tatagtttat ctaggtatga gaaatgaatt taacaataaa
     3601 ttagtttttt agaaaaagag atactagact taaaaaaaca aaacgtagaa ttatctaata
     3661 aagaattaga aaaagaatat gataattcag acgttaaatc tgaagatgtt aaaaaagata
     3721 gtgagattac taatgaggta aaaaaagaat ctgaaaaaga aatggaacag tatagtttta
     3781 gtagtgaatt aatagatgta atggtaaatg ctagtaatga cttaaataga gtatatgaaa
     3841 aaataacaag ctcaggaaca tttagtggat tacaaggtac atttgatact ttatatgaat
     3901 atggattaat gttagaagat ttactagagg taatggattt atctttatta ggaggtaatg
     3961 aaactaaaga gattattcaa aatatagcta tagatttaaa gaatatatct gatgaagata
     4021 atatagctga taaagcatta gatttaatgg gtaatatagg ggagtttaca tactatattg
     4081 agactaacta tagtaaataa ttaaatatta ataataatag ccttgctaca catgactgta
     4141 gcaaggtatt atttttgtaa agaaatgaag atttaatttg gctaaataaa ataaggctca
     4201 actatttgaa aaagaaacta aatattgtaa gataatgaga agcgtaacag ttgaagatgg
     4261 agaagaaaga tttgcaatag aaaaaatata tgttaagtca ttgaaaaggt atgaagttag
     4321 aatatgttta taccgtgatt gtaaggacag aatgaataag ctaataccaa gaccagttga
     4381 tataagccct gataaattat caactttaat attggtaggt ataaaggcgg gtattttaga
     4441 tgatgatttt aagcaagagc taataaaagg tttatcttag gaggtataga atgtcgatag
     4501 aagaatttac tagtggagta atgtttggaa tttctatatt tggtacagta ttaaagaagt
     4561 tattagataa taaaaaatag tacataggag gtgttattat gattggattt ataataggaa
     4621 ctatgatagg agttggatta tgcattagca tagaaattat gatatcaaaa aatacaatag
     4681 tatattaacc tatatttaaa ctgttaatat aatcatatat aatttatttg agtccttaaa
     4741 gcccagaaaa gttttatatg aaggagaatt ttataatatg aaatatacac taaaatatgg
     4801 attaggacaa ttaaaattcg atgtaaaaat aaggttagaa gacatattaa gattgtcatc
     4861 tgcaggagag gataatgatg aagtaaggtt agctgaaaga tcaagagaaa ttaatgatat
     4921 aattggaaag ctatatttaa gaccgagtga aactaagaaa aaaatctaat agctatgaat
     4981 aaagtaaaaa taaatctaaa agtaaatatg atagagataa aattaaggac atattagaag
     5041 aagatgagtt gaatgtaatg gaaaagtatg tattttcatt tgaatcagga aataataagc
     5101 tatttaaaga gtattggaaa gacagaaaga agacagatat accattaata acaacaatat
     5161 tttttagaac tatgttaaga ttaaaggata catatagaca tgatttgtat aacttatggg
     5221 aagattgtaa tctaacaacg gaagaattta aggaaaatat atataaatca caaaatgaat
     5281 ctataagatt tattaaattt aaagttgaaa aaatgataga agaagaaaaa gaaaggttag
     5341 ggaaaagtat taataacaaa tataaaagca atagaaaaat aacagaattg cagaatattg
     5401 atattgataa tatagtaaaa gaattgatta tatctgataa tcctgaaaat aaagaaatct
     5461 atcaaaagat gataattatt acaaaatcat tttttaagat aattgaatat agaagaaaat
     5521 tatcattaag taatcaaaca gcaactgata atttaatgga agaggtaaaa aaactatata
     5581 atacattacc aaaagaatcg agatatgatg cagcatataa gaatctaaga aagttaaaag
     5641 aagatattga atttaagata aaagaaatag ataattataa ggagatatat aaaagagaag
     5701 ttataaaaaa ttgtaaatta gttagtgagt ataaaaaaaa agaagaaaaa ctaaagaagt
     5761 ctggttatat agaagaatca gaaaaaatgg caaaaaaata tatagatata gaaaggcaat
     5821 ttaatgagag tatgagaaaa aaatggatat atatagatat gtatataaga cgtaaggact
     5881 tagaaataga agaaatggat atgaaaaaat atgaagatga taatataacg aattcgatga
     5941 ttaaattagc agaagagatt aactcttaat tagcataaaa atagtacaca gttaagtgtg
     6001 ctattttttt tatgaaaatg aaaaaaattg gataatggtt tggttatttt ttaataaaaa
     6061 attagcttat atttaatttc agaaggaggt aaggaaataa aaatacgggc tataagggct
     6121 ttaggacaac ttagtaggag gaacaaaaat ggaacttaaa acaataagaa ttacatttac
     6181 agtaagtagt gaaacaagtg aagatgattt aagaaagtta tgtaaagatt acatgggtga
     6241 tgagtatgtt gttaatgatg aaggtgactt tgaaatatcc ttttatttaa atacagtact
     6301 tggatacaat gaggtcttat actttatgga gttgattcac atggagctag aaaaaagtat
     6361 tactctatta ggaattagat acaaaggaat aggtggactc caaatgaagc atattctaca
     6421 ggaggaatag agtaagatga ttaggaattg tagtaagata attaagaact atggttttaa
     6481 aataaacagc aaaaatcatg tatttgatag taatggaaat gagatttgta actttgtacc
     6541 aattgtatta ggacaataca tgataggcga tggaagagat cgaaggatag ttattgtatt
     6601 gatgggaata tcagaggacg gtaatcaact agaaactatt aatatagatg ttagcgatat
     6661 agagcatttg aaatgggtta agaatagctg gggatttcaa tataaagtac attctaaaat
     6721 gaagaatcaa tttctatact taatagaagt attagttata aatggaacaa ctaagaagtt
     6781 aagtagtcaa attggatggg ttaaggataa tggaaagtat gcataccttc atactagagg
     6841 tgtgattgga gaaagtgaca aagaagttga tataggtgat cacttaagca attacttttt
     6901 accaagtgaa atagtagata tagacagagc gtgtaaagca agtctaaatc tattagaggt
     6961 agcagactat gaagttactg taccattatt agcattagta tatttatccc ctttacttca
     7021 agctattaag gatgtaggaa agatgccaga gtttgtagta tggttgtttg gaacaacttg
     7081 ttcaaggaaa acttctttag caagagtatt tctgagtcac tttggagatt ttactaatag
     7141 attacctgca acatttaatg acacctatgc atctatagag attaaggcta atgcaataaa
     7201 ggattcactt aacttaatag atgactacgc accaaagcaa actaaaaagc aaaaagatgc
     7261 acaagatgaa atcgcagaaa aggctatacg agcatatgga gatagaatag caagaggtag
     7321 agcaaactct agtatgatta gtcaaaagca atatatacct caaggaatgt tgttaatgac
     7381 aggagagaat cttataagag gacactcaac tgttgcaaga ttagtagcat tagaattgga
     7441 caaggactca gttaatttaa agctactcaa ggaaatgcaa gatagtgtaa gtttgttggg
     7501 agaaagcatg agaggatata tagagtggtt gttacacggc atgaataaag atagtagtga
     7561 catttgagga attctaatac acaactttga tttatataaa gaagaattag agaaaataga
     7621 gtatacacat acaaaaggtg gtcatggtag aagtattgaa tcatgtgcat ggctgctggt
     7681 aggtatatca tccatgttat catattttga agataaaaaa gtaatatctg aagttgagtt
     7741 taatcaatat atacaatttg ctaaagatac tatggtgaat gtattaatta agaataataa
     7801 tatcacaaaa gaaagttcac caatagatga gtttttatac acgataaaag aagcaataga
     7861 ttctaactct attaagataa gtactctagt agatggaaat aaattaaatg acaatgatga
     7921 tgatacatat ggatataagg atgataaata tttttatttt catccagata agacatatag
     7981 ttacgtacaa gagatacaat cgaaatcggg aaactatata agtttaacaa agagagggtt
     8041 aattaaatta ttaagagaac actcgataat taaggtagat agtgatggat ctccatcaaa
     8101 gtatactatt aaagtaaaaa atggaactga ctataaagag aaaagaccta ggctattacg
     8161 agtagctata aatacaatag aaaaattata atacaataag agataaatgg tgatatagaa
     8221 agagggaatt atatgacaat taggattaat ttaaatggag aaaatatatt agaaaatgca
     8281 gataataaat tttatggatt aagggcacgt gttgaatttg atcttgattt attagatgaa
     8341 gatgaagatg taatagatat tatggataga atattaactt tagatgtaaa gccatcaagg
     8401 atgtttataa caaaaggtag agtagatata ttttatcaac ctcaaaagga gattgctata
     8461 caatctaagt caggatatat gaagttaatt aatgaattta tagattttat agaatcgcaa
     8521 gaaattgtag aaatgaagtt ttcaggatgg aacttaaatg gagataaggt tgtagaatca
     8581 caagcaaatg acagtaatat ttcatttagc aaagagatat tcaatccaga tacagtttat
     8641 cttgatggac ttatagaggg atatgtgaat ctttagaaga agctaagtta gaagtatgta
     8701 gttctaatag gagctgcata cttacttaat acaagattat tgaaaagtta aaaaaaggaa
     8761 attattatgg aggagaactt atatgaacta tattacaaca caaggatttg atacaataaa
     8821 aaaggataag atagaaataa aaagaatggt gtatgcttta gagaatgagg gagattcgta
     8881 caatgttatt gaagaatgta tatatacttt agaagaaaat atagatgtaa atgatgattt
     8941 tgtgatagaa gtagaaaatg tatttagttt tgttattaat agaataatgg actttacaaa
     9001 agagaggaat ttgccaaaaa acaaaataga acatgtaaga gcttatatag atgtatttga
     9061 aaatccaact tcaaatacaa aaatagtaaa gtctatagat atatatgaag attatgataa
     9121 aagatatatt gataatgagt tggataagtt atttaaaagc atggataata tgataaaacc
     9181 cgaatttgtt ccagtaatag atggtaaatt tgatatacgt ggaagtaagg atttgaaaga
     9241 gtataataaa aaattaatgt tagacatagt aaagcaaaaa acttatgaag taaataatgg
     9301 aggaataaga ataaataaat tatctgcaat attaaaatta ggagataaaa gcatagatgg
     9361 cataagcaaa atgatgaagg atgtggcaaa tctaaagata aaaccaaaat cagtatatat
     9421 aagtgaagat acagttgttg taatgtatat atgtaataag agttgtatcg tgcttaatat
     9481 agaagagtat tccaagatac tgtcagattt tgttgactac ataaataata tagaagagat
     9541 ggacttatat ttagatgata gcattttata tggtgactat aaaataaatt gtggagtaga
     9601 cgatagagat ataattgatt ttaattatca aagctttaat tcaaaagtaa ctttgtttgc
     9661 tggacatata ggaaacaaga taatacaagc tgcattatag aaatatacaa tcgtagagag
     9721 gatatagata tgacagaaac ttataaaatc agcataggaa gaaaagattt atattctaaa
     9781 gactataagg cggttgaaag aatcgactat gaaaagaggt atatagttaa tataagtaga
     9841 agcttagtag acatactttc tatacttata gaagaaggga tataaattat agatatggct
     9901 acatacttag tgtgtagcct ttaatataga cttaaattag tatatttaaa ctttggagga
     9961 tataatatga aatcaaagaa agcagtagta tatgcaagat ttagttcgga caatcaaagg
    10021 gatgagtcaa tagatgctca attaagggca atcaatgaat atgcagataa gaataatata
    10081 aaaatagtta atcagttaat agatagagca aaatcagcaa catcagataa aagaccagaa
    10141 tttcaaaata tgattaagtt ctgtgaagca gatacaacag gaatatctat ggtaatagtt
    10201 cataagctag atagattttc tagagataag tatgattcag ctatgtataa acagaaatta
    10261 aaagtaaaag gaataagagt tgtaagtgta ttagagaacc tagataattc gcctgaatct
    10321 ctaatattag aatcagttat agaaggtatg gcacaatact atagtgctaa cttagcaagg
    10381 gaagttgcta aaggtcaaaa agaaaatgct cttaaagctc tacacaatgg aggagatgca
    10441 ccattaggat atgatgtagc acatgataaa acttatacag ttaatgaaga agaagcacaa
    10501 gcagtaaaga ttatatttga tagatatatt aaaggatatt catatagtaa gataatagat
    10561 gaattaaatg atttaggata taagactaaa agaggtaata aatttggaaa gaatagttta
    10621 catggaatat tgaataatga gaagtataca ggagtatatg tatttaataa gactcagaga
    10681 aaaggtgtag atggaaaaag aaatggtcat aagcaaaaga gtgaagatga aataataaag
    10741 gtagaaggtg gtatgccagt tataattgat aaagaggtat ttgaacaagc acaagagatg
    10801 atacaaaaaa gaaagaaaac tccaggagca cataaagcta ctactacata tttattaaca
    10861 ggattaatta aatgtggaga atgtggacat gcatttcagg ggaataagag aaaagactct
    10921 tatggtaata tatgtttcat atagatgtgg atgtagaaag caaaaacgag attgtaataa
    10981 tagagaaata aaacgagatt atctagaaga gtttatatta caagaacttg aaaagaacat
    11041 attaaatgat gaggcaatac cagctttaag taaagcattg aatgagaagt tgaattataa
    11101 gaatgaaagt aatgcagagt tacttaaaaa cttagatcag aaactagaga aggtaaataa
    11161 agagataagt aatatactaa atacaataat gaatggtata gtaagtagca tgttaaaaga
    11221 taagcttgat gagctagaac aagtaaagtt aaacttagat ttaaaaatga atgaattaaa
    11281 gatagaaaat aaagttgtag atggtgtagg cgtaacagaa gaacaaataa gaagtatgtt
    11341 ctcaagattt aaggaatttg tgctagagag gaacatacct gaatgtaaga aatttatagg
    11401 ggactatata aaggaagtaa tcgtatataa agatcacgtt gaagttgtat tcaatgtggt
    11461 cttttctttt gtcgatgatg aaatagctca taacttacac attactgtat caagagtaaa
    11521 agtaaaataa aaacattaaa tataaatgtt aatttaaatg atattatgga tatatttaac
    11581 aaataagtag tgttacatga tataatatca catgtatatg aaagggataa aatttatact
    11641 attagtattg gtggtgaaat gaatgtcaaa aattgaatac tatatgaatt taccctatga
    11701 tttatcagga tcaatgtcta agaatagatt tagaaatgaa ctactttggg gattgaaaaa
    11761 aatgtatgag ttatataaga cgaatgaaga ttttgtagta gtttttgatt atagatgtga
    11821 tattgaagtt catttagaaa ataaatttga attttatcaa gtgaaaactc aaaataattc
    11881 aggtacatat acgataaata aacttactga taaaaaaaaa ggtagtaaat cagtagtagg
    11941 gaatttatat agcttgaaat atgatataaa tatgaatgag attgatgatg tttatgttgc
    12001 aattgtatca aatgctcctt tgaacgatgg taaaaaaaca tataataatt ctcatgaagt
    12061 taaaataaaa acaatagata gtagtgctat agaaaagata aagttaagta taaaaaatga
    12121 cttaaatata gatgaagata taaatttaga aaatttattt tttattagaa ttggtttaga
    12181 tttaattgaa ccacaaaaaa ctttaattgg tgaaactgct atattttttg aagatgtttt
    12241 tggaagggaa tgtagtaaag taaaatcatt atacaatgtc ttatatgaag agatatatag
    12301 caaatcagca tatgagttag atataaatac gtatgaaaat atagtatcta agaaaggtat
    12361 taatagagat aggatggata ttatattaaa aagttatgct aataatacag ataagtcagt
    12421 agaactagca aaatatagta taaatgaatg gtacaagaat aatttctcac gtagattatc
    12481 tttgataaaa tcattaacta caattgtatc taaattaagt gaaagtagta ttttaaagga
    12541 tatagaaaac tgtatattgt catatatata taataatata gataaattac aagtatcaga
    12601 taaagagtta attgaaatta taagtgagat agtagaagaa aagaggccta tagaagttag
    12661 caagacagat atagaagctc ttatcttact aacattaaaa aagtatgagg agggtgttta
    12721 tgaatagttt gtttgtgaac agtctatata tatttgaata tgaaagtaaa atggcgaaga
    12781 aaatagactt taaacatggg attaatatat taacatcaga tgaaaagagt ggtaatgacg
    12841 taggaaaatc agtactattg aaaagtattt atcacacttt aggagcagat agtatatttg
    12901 atcatatgtg gaataaaatt acaaaaacat atatattaaa tgttactata aaaaataaga
    12961 catactatgt atatagaaga gataatttat ttaaaatata taatacgaat tttaaaaaaa
    13021 tatttatgac aaataaccgt gatgaattat ctaattattt aagtgattta tatagtttta
    13081 aggtaatgct gccaaaaaga gaaaatgaag aattagagtt agcacctcct gtatatagtt
    13141 atttgcttaa ctatatagat caggatcatt tagatggatc taaattttca tcttttaatt
    13201 cattgacaca atttcctaac tataaggaaa atgtaattta tactcatttt ggactttttg
    13261 atgaagagta ctataaagct ataaaggagt tagaaatatt aaaatcagaa gaaaaaaata
    13321 agaaaaaaga acgagaagtt actttgaata tgctaaataa aataaaaagt tacttaggaa
    13381 acttagatgc accagaaagt atagaagttt taaatataga attaaataaa actaaagaag
    13441 aatattcgaa tatagttata aacttgcaaa aaataaaaaa taatttaatg aaaataagga
    13501 atgaaaaagt agatttagaa ttaaacataa aagaaattga attaacaaga aggaatgaaa
    13561 tgaagaagtt aaaagttaaa aatgtgtgcc caagctgttc acatgaaata gaagatatag
    13621 attctaaatt tataataaat aatcaaatag aagatcttat tattcttaaa gatgagttag
    13681 agtcagaaat tataaagtgc aatagagtta tagaaaagaa agaggaagaa tatcaatcac
    13741 tattagataa acttcatgag tatgagtcaa atatgaaaat aaatgatgat agcatatcta
    13801 atgtcttaaa gcataaggca tatatagata ctaaagatga tatagtttta gatttagaaa
    13861 taatagataa aaaaataaaa gaaatacaac agacaataga gccatatgat aaaaaaataa
    13921 aagaatacaa cagtcttaag aagaaggcaa atgctaagta tgaagagtta atgaaaaagg
    13981 ctaaaaaaga ctttggatta gaagagatag atgaaaataa aataaaaaat ataaaaaata
    14041 actttatagc aagaggtagt aatagagcaa taactactat aatgtggtat ctgacattgt
    14101 tagcagtaaa atatgaattt aatgaaaatg caataaagtt tccactaatt ttagatagtc
    14161 caaataatgt tgaatctgac gagataaagg aagaatcatt gtttaaatat atatttaaaa
    14221 atactaaaac agatactcaa ttaatattat catcgttagg gttcaatgaa atgcaattta
    14281 gtgaatttaa gtttgataat ataataaaac ttgaaaatag caaatataat ttattaaata
    14341 aagaggatta ttataataat atagatattt taaataaaat atttgaaaat tagtatagga
    14401 atcgtgggta tgatttctgt gaatcgtctc caccattgaa atccacgaaa acacattgat
    14461 attttatcaa tgtgtttttt aagtaatagg gggaaatatg aaagatagat ttaacttaaa
    14521 aagaaaaagt attcagtcat taatgaataa aaatgtagat gtttattctt gtaaagattg
    14581 tatagatgat tttatagaat taaattttaa aatttataga agttattata tatcagatga
    14641 tgatatatta gagattatgg atggtacagg agaggactac aaggatgtta tagcagatat
    14701 gttaccagac aaagcgcata taaaatctgg agaatttggt gaaataacag caacagaaat
    14761 atataagagc aatatacaaa caacagaaat atatatagtt gataaattaa gtcatacaag
    14821 aaaagaggat actaaagttg ctgcacataa aacagatatt ttaattattg aaaaaacctc
    14881 agaaggtttt ataattcatt cttctgaggt taagactaag ataactgatt cagactttaa
    14941 tcctattgaa gcaatgatag agggggtaaa tgatgatata gttacacgtg ttgcagagac
    15001 actgaattgg attcaaagaa aactgaaaac agaaaagaaa tttgatgaat taaaatttat
    15061 aaaacaagta atagagagta tagaaaatca aaatgtagaa aatgaataca atggtgcagt
    15121 ttttatagat gaatcaaaat tagactacga gataaataaa aatatctatt gtgatgttaa
    15181 gctttctaaa agccaaaaag gcaaatatga caatgaatta aagtctatag gatgtgtcat
    15241 agatggtgat aagattaggt ttgatagtgt gaacatagat gatattaata aactaaatat
    15301 taatagtcgt aaaagaaatt caatagaaaa attatataat aaatctcaaa gaaaactaga
    15361 acatatagat gatattaaaa ttcaaatagt aacttttgat gatattaata aacattgttt
    15421 agatatgtat gagaaaatta ttcatgtagg gagaatataa tgagtattaa taataatata
    15481 aataagatta ggaatcttga tatacaatta gcaaaatatg attgtatatc aagaatgtct
    15541 gcaaaagaaa catcgaagca ttatatgcga ttatacttta aatttataaa attattagaa
    15601 ctatatgaag atagtagttt aaatataaac aatggagaat acttaaatct agctaatgac
    15661 ttaataatat tttctaaata tgatatgtcg agtagattta aaggaattga tgaagaaaga
    15721 aacttagtat atatagcagt tatatattac atattgggat atagatttga gtgcaactta
    15781 atattagata tattagatat gcatgatata gaaaatgaat atttaaaaat aataatttat
    15841 ttaattagaa ataaatatta taaatgtgat gtattatctt gtataaaaaa atattatata
    15901 gaagatgaga gtaatttaag tttaataata aaaactttgt atgaaaaaga gcttgtatgt
    15961 gatactaaag atcagaccta tatacaaatt attattgcta tattaagaaa tatacaaaat
    16021 aatggattct atagagttat ttcaaaaaaa tcaaaattag atataaatat atggagaaaa
    16081 tttgtgcaac aaagtataaa gtatgatcaa atcatatatg atcttcccaa atatcattat
    16141 gatttattaa atgatgaatt attgaatata aagcatggat atataaatat gcccactaat
    16201 tactctaaga tagaagtaat taagatagta atatttaatt atttacatac atatcaagat
    16261 aaaaaaatag catatataca tgtagataaa gattataggg ctataaatgc aattaaagaa
    16321 tattttactg aatattcaac aagaattgaa tttattgaaa tagattcaat taataattat
    16381 agtgagatag ctactgataa caattttata atcatagatg attttagtaa tgtttcaaac
    16441 aaatactatg gtacacgatt tgaaataatg atatcattag cattaaaatt agaaaaaagg
    16501 ctattagtat tgaataataa ttatcttaaa atggactcta atcaacttga aaatataaat
    16561 tataatgaag atattaatag atatagcaat atagttaatt actattttaa agatggaaaa
    16621 aataaagagc ttgaaataga atatgaaaaa gagagatttg ttacagaaag ttatttgtta
    16681 aataaggaga taaataacaa aataaactta aagggtatta aattgaacag tgcaggagat
    16741 aaaagtttac tatctgcaat aaatttaata tctaaaggat acaaagtatc aatatttata
    16801 ccacagaaaa attctgcaaa aagtggtata aataaaatat gtataaagct attaaattta
    16861 attgataggc ttaacggtaa aatattgtac gataaaaatt gtatagatga tttaaactta
    16921 ttagatagag cagagttatt gttaggtaga gaaagtatat atatgaaatt atttagatta
    16981 ggggtaattg taattcattc tgatatgcct caagaagtaa ttgaatatat gagatatttt
    17041 atacaatgtg gaaaatataa
//
LOCUS       DAEMAE010000067        16976 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEMAE010000067 DAEMAE010000000
VERSION     DAEMAE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.068"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(213..3548)
                     /locus_tag="KPH93_001769"
     CDS             complement(213..3548)
                     /locus_tag="KPH93_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG1688594.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVCGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGNTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3598..4026)
                     /locus_tag="KPH93_001770"
     CDS             complement(3598..4026)
                     /locus_tag="KPH93_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688595.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(4016..4396)
                     /locus_tag="KPH93_001771"
     CDS             complement(4016..4396)
                     /locus_tag="KPH93_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG1688596.1"
                     /translation="MRNISNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4429..4737)
                     /locus_tag="KPH93_001772"
     CDS             complement(4429..4737)
                     /locus_tag="KPH93_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688597.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4770..5942)
                     /locus_tag="KPH93_001773"
     CDS             complement(4770..5942)
                     /locus_tag="KPH93_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG1688598.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISE
                     VLDKAQELIDKRKEQIEVLDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKGELFN"
     gene            complement(5946..7409)
                     /locus_tag="KPH93_001774"
     CDS             complement(5946..7409)
                     /locus_tag="KPH93_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG1688599.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKSDGYS
                     LDDKRNEIKDSDIEDIIERFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(7815..8687)
                     /gene="gatY"
                     /locus_tag="KPH93_001775"
     CDS             complement(7815..8687)
                     /gene="gatY"
                     /locus_tag="KPH93_001775"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG1688600.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKCY"
     gene            complement(8707..10623)
                     /locus_tag="KPH93_001776"
     CDS             complement(8707..10623)
                     /locus_tag="KPH93_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG1688601.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPTSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSVSGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNKSAESENVFNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(10626..11546)
                     /locus_tag="KPH93_001777"
     CDS             complement(10626..11546)
                     /locus_tag="KPH93_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG1688602.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLEKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKNIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVISSACAGDSALAAFLSIWLENSEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11648..12400)
                     /locus_tag="KPH93_001778"
     CDS             complement(11648..12400)
                     /locus_tag="KPH93_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1688603.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(12792..14237)
                     /locus_tag="KPH93_001779"
     CDS             complement(12792..14237)
                     /locus_tag="KPH93_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG1688604.1"
                     /translation="MIKLGKFPKGFLWDGAVAANQLEGAWNEGGKGWCLADIHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIIIAENGFGMHDKFEDGKV
                     HDSYRVDYLEKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERCKKDSFYWYQNVIRTNGEELREK"
     gene            complement(14259..14612)
                     /locus_tag="KPH93_001780"
     CDS             complement(14259..14612)
                     /locus_tag="KPH93_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1688605.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKEFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVRSIEYKVNNSK"
     gene            complement(14641..15888)
                     /locus_tag="KPH93_001781"
     CDS             complement(14641..15888)
                     /locus_tag="KPH93_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1688606.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMRNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTIVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEI"
     gene            complement(15896..16213)
                     /locus_tag="KPH93_001782"
     CDS             complement(15896..16213)
                     /locus_tag="KPH93_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688607.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            16455..>16976
                     /locus_tag="KPH93_001783"
     CDS             16455..>16976
                     /locus_tag="KPH93_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG1688608.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYREY"
BASE COUNT         5368 a         3218 c         1728 g         6662 t
ORIGIN      
        1 tacttaattt taaaaattat tatttaatct tcttcatgag taattcatag ttatttatca
       61 attaaaaaga gatattgcat aattattcaa tacctctctt tgtatattta cattatccat
      121 atctagttat ctatttcaac ccaatattag actacctact aataaattta ttctctacat
      181 cattaataat aattttgcac taaataacat cattagccta tctctgatgc attttcttta
      241 attttattta tagttttaat caatttattt ctctcttcaa tatgattttc aaataaatca
      301 attatacttc caacagttct aaatgggtct tccatcaaca ctttgttatc ctctataaat
      361 ccattcttga ctacataatc gactataagt ttaacaaaat gtatttgctt agtattaaaa
      421 ctcttattat ttaaaaactc tgaaaaagct tcatttgcag cttctctatc caatcccact
      481 agttttctca ctaattgact tactggagta tttccaaact ccttcttata atcatcacaa
      541 ttacctaact gctttaagag tatttcctcc aacgtttcaa catcttgctt tgttatcttt
      601 ttattatttc ttaatttaaa tattgctaat tcatctttat gactatttaa ataatactct
      661 accttctttt tataattttt aagattgttt gcattatata cagaatcatt tctatcttct
      721 gctacaatca tatcttcaaa acttgtataa tatatctttt gactagactt ttcaagatac
      781 ttaataagct ctcttaatga tatacgtact tcttctacct caaatatatc tgaatcttcc
      841 caaaaatcct ctgtcattgc tttttcaata gtatattttt gttgctgaat ctgtggtata
      901 gtcccaagtt ttgaaagttt ttctgctgta gccattacat gccttatacc tttacttgca
      961 ttattatttt gtaagtatgc aagttgtata ttatacataa ctacatcaaa tcttttagcc
     1021 aattcatcat cactatatga agttatgagt ggtgatatat attctcttat ctcattttgt
     1081 gctactgctc caatattact ccaaacattt tcatttttat acttatgtac ataacttaaa
     1141 ttcatcctaa ctatataact atcctcattt aaagctttta cagaagttat aagttcttct
     1201 aacaattctt ttctaagttc tgcatactca ccttctatat atttaatatc ttgaagttct
     1261 ttaaccatac ttacctttat attaaataat ttttcagtaa gcgtttctgc tttatttccc
     1321 tcaaatcctt taggattaaa tttaaaaaat tcaaaattat tacaaaaatc aaatattaag
     1381 aatctttcct tatccatatc aacacctaat aaatctggac ataatctagt tcctctacct
     1441 atcatttgcc aaaacttagt cttacttctc actttcttga aaaaaacaag atttagtatt
     1501 tcaggtatgt caattcctgt atcgagcatg tccactgaaa ctgctatttg aggtaattta
     1561 ttcttatcag aaaaatcatc tattatagaa tctacataat ttatactata gtcaactgct
     1621 tttacaaagt tcccaccata ctttggatac agcttattaa atctttcaac tattgcccta
     1681 gcatgctttg tattctttgc aaaaattata gtcttaccaa tcttttcttc tccttctatt
     1741 ttcaacccct ctgtcattag cttatttaaa actaaatcta ttgtgtcaga attaaatagc
     1801 cactcattta ctgcattatt ccctatttcg tctccaatac tctcatcatc attaaatgta
     1861 ttttcatatt cttccttctc ttcatcagaa agctcatcat acttaattcc atcttccata
     1921 atcttagttt taaactcaat actagtataa tctactaaat atccatcctc gactgcttta
     1981 ttatactcat aagcatatgt aggtactcca ttttccatgt caaaaactga ataagtattt
     2041 ttatctatct catcctttgg tgtagcagta agacccatta agtaagaatc aaaataatca
     2101 aagattgact tatacttttt ataaatactt ctatgcgatt catctattat aattaaatca
     2161 aaatgaccag gtgtaaacaa tctctttcca tccttagact tcgtatcatc aattgcattc
     2221 atcattgttg gatatgtaga aaatatcatc ctagcatctt ctgggctgtc tttgctatcc
     2281 aataaattac ataacgccaa gttaggtaat agcttagtaa aacttttttt agcttgcttt
     2341 accaatgctt ttctatctgc tagaaataat atatttttaa tccaattatg tcttgaaagt
     2401 acatcaacta tagatatagc aattcttgtt tttcctgtac cagtagcaca taccaaaaga
     2461 gtttttcgtt gcttattttc aagagcctca catacagccc taatagcttc tttttgataa
     2521 tatctattgg atatttcatc atttattgtt acagaagtta aagacttctt actctttctt
     2581 ctatctatca taagttgtaa ttctgatttt ttataaaatc cacaaacttt cctatctgaa
     2641 tagtcatccc aaatatacat atcaaaacca ttagtcaaaa atattactgg tctttgacta
     2701 tatctctttt ccaaacaatc tgcatataat ttagcttgtt gttgaccgat tttcgggtct
     2761 ttactcgttc ttttcgcctc aactactgct aatggcttac cattttcacc ataaagaaca
     2821 taatctacat atcctacacc actttcatta ggcatacctt gtacttctat ttcttcccct
     2881 atatcttttc caaattccca acctgcaagc tttaattcta tatttatata tctttttctt
     2941 gtctcaaatt cgcttacttc gtctatcttg aaatcatagt ttacagtatt aacttttctc
     3001 ttttcagtta gtgactttct taattcctca ttttctttta ttacttcttc aagcttttta
     3061 tcttttgaac ttaatctctc ataaagattt ttaagttcgt ctggtctcgt ccttttctct
     3121 tccccatgaa gtaataaatc ttcattaaag tcttctgctg tatattcatc tgaataacaa
     3181 tagtctatcc aactaacaaa ttgatgcaaa ttgttaagtg aaagtattgc ttctcctctc
     3241 tctatgttcg cactagtatg tacagataaa ttacctaatt taactatata ttttaataaa
     3301 ggtaacatat catagtctat aagttctata aaactattgt tatgaatcaa cgtactaaga
     3361 ttatcttgat aaggcaatac taaatcacta tcattagcat atagccattt tactgcaagc
     3421 tctaaagctc ttcttgttat agttgcacaa gtagatgggc taactagtat acttttttct
     3481 gcttctaagc aagcagtatt aaaactattg aaatttttct tcttttgtaa aaaatcaaaa
     3541 ttacccaagg cttttctccc ccttttgtcc cttagttaaa ataaatctct ctaaaaacta
     3601 tttcttacag ctgtttctta tactaacaaa tacttcttca ttttctgtaa acaattcgta
     3661 catatcttca aactgtggta tatcatatcc atgagaatca tcatttctca attttctaag
     3721 tgtttctata actatagaag aatttttagg taataaattg tgatactcac aagctaaaag
     3781 gtggtcataa aagtttttag attttatgtt taaatcaatt gatttataat acaacattgc
     3841 ttttgttaac tctagaaatg agaccatata gcttttaaaa ccttctttta tatatctagc
     3901 ccataactca ggcatttctt ccttatcata taagattttg aagttttgca gatcatacaa
     3961 gcaagaacca aaatccctat ttacttcttc tattgagttc caagataatt tgtttctaat
     4021 taccatacat gtccctccaa tctaagatat ctattacatc actgttttca aaatagaatt
     4081 tatttatata atccttaaaa tctttagatt cataaataac ttcaccttct aaaatagctt
     4141 ttttaagtgc tattgtataa ttatctgtaa caatttttag gtctacatca atgtccaata
     4201 tatttcttag agcttctgtc ttagttgcaa gttctgtaat tggtactttt ttgatgggaa
     4261 tccaagctat gtctatatca ctttctgatg tatgtttatc tgttccataa ctaccaaata
     4321 ttatgattgc tagagtattt tcttttggaa atacatttat tattgagttt ttaatctcat
     4381 ttgatatatt tctcatttaa tcacctcttg tttattaggt aatattgctt aattgaattt
     4441 atatccataa tcttttaatc tatttataca tatttctctt tcttcatcag taaacaactc
     4501 tttatacttt tcttttaata ctaatacttc taatgataaa tctaatctct tacactccca
     4561 tagccttgta aatccttcag tagctccttc tttattgatt aattctttag cagctaataa
     4621 ggctcctttg ttatttatca gttgtaaaaa tcttgttggc ttatagttac attgtaaatc
     4681 agcttcttta tagatattta ccatgtcatg atataaatct aactctattt gattcataga
     4741 ttttattccc ccattttaat aattgtattt tagttgaata attctccttt aaaagctttt
     4801 tgcattaatg agttaaagtt gtcttctagt tcttttagac ttgtttttat tcctactttc
     4861 aatttgtcta cttgattaac aaactcttca aacttatttt gaagttctat tgatggtact
     4921 ggtattaata ttttttgaag ttctttgctt ctaattcctt gtgcagagct accagtttta
     4981 tatttatcta tttctaccct aaaagctttt gtttgcatat attgtgctaa aaaagcatta
     5041 tttaaaattt ttttatctcc ctgaattgtt ataatccttt gacccatagc aaactttttt
     5101 aaatcctttg gtatcctaca tacatttcca aatgtatgac cttctgtaac aaaaagtaca
     5161 tctccagcta ctggaaaacc tcttttcatc caagaattat aggtgttttc agatatatat
     5221 tcattaatat atttaaatac accattccct acagattttg catttattat tctaattcca
     5281 ttctctgaaa gaattggagt tttaccacga taatcaataa aatttgtaca atctttcaat
     5341 aatacaaact tttgttcctt aaaatttcca aacatttcaa taaatcttga tttaactaat
     5401 tcatccagta cttctatttg ctcttttctt ttatctatta actcttgagc tttatctaaa
     5461 acttctgata tatctttttg agcttccatt tcaggtaaat ctacttttaa attttttaaa
     5521 tcattaactg aaaaacttgc ttgattaact gattttttta ttatccttgg taactgtctt
     5581 ctaaattcat tactattaaa aaaataattt aaatactttg gataacaaac ttctttatct
     5641 ggtcgtaaac ataaaagatt cattccatgt ataatttttt tatttatatc ttcatatatt
     5701 gcacttttcc ctaaatgttt ttcactatta atatgtgaca ttagtatatc tccatttttc
     5761 aataaatagt cttctaattt tcctaactct tttatgtcag catatcccat tttattagta
     5821 tcaatataag agtttgctat agtttctatt cttgtaattg gatatccaaa ttttgtatca
     5881 aattgtttaa tactagcccc atttctaata aattcaaaaa cttcattcaa tctatattcc
     5941 atactctaca cctcaagcat tctttctaat tcctctaatc catctacgat ttccttctct
     6001 aactccctaa ctctacctat aataacacta ggagcatcat aaactacttc ttcatacact
     6061 acctctttat acttattaat agataaatca tacccattct ctcttatctc ttccacatca
     6121 acaaaaaaac tttgctctgt tctttttcta tctacttccc catcaagatt atcaaatctt
     6181 tcaataatat cctctatatc actatccttt atctcatttc tcttatcatc caaagaataa
     6241 ccatcagatt tcatatcata gaaccatacc ttatcagttc caccagttcc tgttttagta
     6301 aatatcatta tagctgtaga tactcctgca taaggcttaa acactccact tggcattgat
     6361 attatagctt ccaatttatg attatcaact atttcttttc ttatcgattt atgtccacct
     6421 gttgaaccaa acaatactcc atctggaact atacatgcgc atcttccacc aatttttaaa
     6481 actcttaaaa aaagtgccaa gaataataac tctgttttct ttgtcttagt cacttttaat
     6541 aaatctgctg acactgcttc ataatctaaa cttcccttaa aaggaggatt tgctaacacc
     6601 aatgtaaact tatctttatc tgtattaacc tccgataaag aatccttata ctctatatta
     6661 ggattgtcaa cgccatgcaa catcatattc atagcaccta ttctaagcat agttctatcc
     6721 atatcaaatc catagaacat accattatta aaatgctcct tcagcccttg cactaagaaa
     6781 agacttgaat gattatccct taagtattgt tgtgatgata ctaaaaaacc tgctgaacct
     6841 gctgccgggt ctactataat atcttctggt gttggtttaa taagtcttac tatcatatct
     6901 attatgtgtc ttggagtcct aaattgacca ttagtacctg ctgtagccac tttggataat
     6961 aaatactcat acaaatctcc ctttgtatct ctatccttac ttaattctaa attactaata
     7021 ccatcaacta ttttagataa cataagaggt gtaggaattt taaatattgc atctcccatg
     7081 tactttgcat aagctgattc tttatttcca tgtaattttt ttatgaaagg gaacacttca
     7141 ttttgtacta tttcatacat tttttctgag tttccttcat tagaaaactt actccatctt
     7201 aaatgttgat gttcatcatc aaatatcttt tcaaaagaaa cacccaaaag agttgcttct
     7261 gcttcatttt tggtttctac ttcatccaat cctttaataa ataaaagata tgtaaattgt
     7321 tctataactt ctaatggatt agttaatcca ccagtccaaa atgtctccca aatcttgtct
     7381 actttgctcc ttaattctcc tgtaatcata tatgactttc cctccagtgt tcataaaata
     7441 atttaaatat atattttagt cgtacgcctt aatagttagt gtcttatgtt aaatccaagt
     7501 ttttactaca cagttattaa attatttttc ctatttacat attttagcta aaaaactaat
     7561 gcaatactct tattttataa ttattatata taccatacaa aattatttta tcatatatcc
     7621 atcatagaag tcatctacct ggataactct taccatatta taacttttta atattaattt
     7681 aaacttagaa agaaattttt tattttcgga atgatgaaaa tatatttaat ttcatacaaa
     7741 aaagagtgcc tcctgaacta aaaagttcat tttgagacac ctcttaattt aatcttactt
     7801 attctttccc tttattaata acacttcact atctctattg cttttcctac acatccacaa
     7861 acttccatcc tactcttaac aaaatcttta actgactcta aacctttttt accatatttc
     7921 ttagggtcaa ttacctctgg gtcagaatta agcacatcct taaccccatt actataagca
     7981 atccttaatt ctgttgcata attaaccttg caaatccctc tttttataga ttccttaata
     8041 acttcatctg gtattccaga cccaccatgt aatactaatg gaactgatac aacctctcta
     8101 atttcagata atctatctaa atcaagcttt ggctctccct tatatagccc atgtgccgta
     8161 cctattgcta ctgccaaaga tgatacccca gttctctcta caaactcttt tgcttctaat
     8221 gggtctgtat atgctccacc atcaccacca tctaaatcgt cttccttacc tccaacttta
     8281 cctaattcag cttcaacagg tatatctgaa ggcatacatg catctacaac agatttagat
     8341 attgaaatat tatcttcgta actttcatga gaaccatcaa tcataataga agtataccca
     8401 actctaagtg cctgcatagc tagtccaaag cttgaaccat ggtctaagtg cattgccact
     8461 ggaacacttg ccttctctga tgctaccttt acattcgcta aataataatc tagtcctgca
     8521 tactttattg ttgaaggtgt tgtttgcaat attactggtg atttaagttc ttcagcagcc
     8581 tctattactg ccataaccat ctccatattt tcaacattaa atgcacctac tgcataatgc
     8641 ccttcttgtg catctaataa tatttgttta gttgtaacta atgccataat ttctcctcct
     8701 atatgtttat actgatttga tttcttcgtt atttaatttt gatactattt ctctacttgt
     8761 tttgctattt aataactcat ttaaaaattc ttcattcatt aattttgaag atagttgaga
     8821 taatgttttt aagtgtaaat tatcaccatt atctgtagct gctatcataa atataatatg
     8881 tgccaagctt ccatcttctg attcaaagtc aaacccttct tttgatacac ctactgctac
     8941 acgtggtacc tttactgcat ctgtttttgc atgtggtatc gctatcccca taccaaaacc
     9001 tgtagatgat atttcttctc ttttaaggat ttcttcttta aacttctttt tatctaaaag
     9061 aacaccagat ttatcaagta ttccaatcat ttcatcaatt acatctgttt tattttttcc
     9121 cttaacatct aaaattactg tttcttcgct atataaagat atattattaa atacgttttc
     9181 cgattctgca cttttatttt tatgttcatc tttagttgca tttgtatttt ctatagtatt
     9241 tttctcattt actactttag gtgtaaccat catataaagt acagcaccta ctaatgaacc
     9301 tacaaatata caccctaccc ataaaagtgg cttattgact aatcctagaa ccaaaaaccc
     9361 tccatgtgga gctggtactt ctattttcat tatgtaagtt aaaattgatg atattgatga
     9421 tgcaatcata agtactggca acacttttag tggatttttt gctgcaaaag gtattgcccc
     9481 ctctgttata tgagtactac ctagtatata attgattagt cctgctgctc tatcttcttc
     9541 tgtaaatcta tttttaaata aagtagatgc tattgctatt accaatggtg gtgttataca
     9601 agctgcagat acacctgcca tgaataaata atttccctgt cctagaagca tagttccagt
     9661 tacatatgct gctttattta ctggacctcc catatcaaat gctgacatac atcctattac
     9721 tattcccaaa attatagggc ttgaattttg taaccctgat agaaagttca tcatagcatt
     9781 gttaagtgct gaacatggcc cgccaagtaa caacattgct acacccacaa gacctacgct
     9841 gacaatagga attataaaaa tcgatttaag accttctaat tgtcttggta attttctcaa
     9901 caaatgtaca aatagtttga ctaagtaacc tgccaaaaat cctgatatga ttccacctaa
     9961 aaaccccgaa cctgtatcga atgatatcat tcctccaaca agacctgcca acataccagc
    10021 tcttcccgat acagattgag ctataaatgc tgccaataca ggaaccatca agttcataga
    10081 atatccacca acagatttta acgtagctgc tatttgatta tattgttctg aagttgggtc
    10141 aaatgaataa atcccccata aaaaagatat agcagtaagt actccacccg ccactactaa
    10201 aggtaacatg tgagatacac cattcattaa gtctttatac aacttttgac caaacgataa
    10261 atttccttct tctgcaattt tactgcttga taagtttcct tttctaatag ctacttttcc
    10321 atcaatacat ctttgtataa gttcattagc tttatgtata ccttctttga caggtacttc
    10381 tattactggc tttccattga atctgtccat atcaacattt ttatcacaag caactattat
    10441 tgcatctgcc atttcgatat cttttgctgt taacttattt tctacaccta tagctccatt
    10501 cgtttcaact tttatgtcac accctaattc cctggctgaa ttttttaatg cttcttctgc
    10561 cataaaagta tgagctattc cagttggaca acctgttacc cctagaatca ttttctttcc
    10621 catttttata ccacctttct gactcttata ttttttatat aatcttccac attctccaaa
    10681 ttacctatac cattactttc agccacactt gctcctgttg ctgctgacct ttttaatgct
    10741 tcctcaatat gttctgaatt ttccaaccat atacttaaaa aagcagctaa tgcagaatca
    10801 ccagcacatg cagagctgat tactgtaact ggttgagcac tagcataata aattgatttt
    10861 ccattataaa aataagaacc tttttctcct aaagttagca atatattttg agcacctttt
    10921 aaatgcagta atttaagtac atccttaata tcttcttcat ctcttacaat aattccaaat
    10981 atatctttga tttcttcatc atttggtttt atcaaatatg gattatactg tagtaattcc
    11041 tttaattttg gtgaacttat atcgagtatt gtctttatat ttttattttt acaaatctta
    11101 aatatatcct catagtaatt atcatcaata ccaggaggta gacttccact tattgataaa
    11161 taacttatat cttctaatga ctgtatcttc tctagcatat ctagtttttg tttttcgttt
    11221 acatatgacc cagaatttac aaatttaaat tcatcacttc catcatttaa aaatatattt
    11281 atacgagttg tatcttctac ccaagtaggt aatacgtcaa atcctctctt ttcgctttct
    11341 tcaactatat attttcctga aaatcctcca aaaaatccta atatcttaga ttctatacca
    11401 taatgaccta gtacaaaagt tacatttaat ccctttccat taggagaata tacagcattt
    11461 gatgtacgat ttactaattt tctctttatc ccattagtag atatattcat atctattgca
    11521 ggattcgtag ttaatgtata tatcattgtc ttcccccttg cttgtgctgt gtatgttttc
    11581 attataataa aaaagtctat gaaaatcttt tcacaaatag aaaatcgatt ttcatagact
    11641 tttcaattta attttcagaa ttattatatc tgaccatata atctataatt atcctagata
    11701 taatatacaa gggcaatctg gaggatactt catattcaga aattgatata tgttcatgtt
    11761 tgtatcctac aatatataaa tccgaatact ttataagttt tgcactttca ctacaacagc
    11821 aagttataac tttagctcca actaaattgg cattctgtgc tgattccaca agctctatgg
    11881 tatttccatt caatgaaaat ataattataa gttcatcctt ttttattgat ttagatttta
    11941 ttttcattat gttggggtca tttataaaaa caacattaaa atcaagtaat tgcaatttaa
    12001 gactaaactc ttttgctaca tattcagtaa gccctcttgc tagaatatat atcttactag
    12061 atttatttat agtttttatt atattgatta gtgtagttaa agatatttgt tctataactc
    12121 tctgtgcttc tataaaggat ttatttatta tatcccccat attttgtata tcatcatttt
    12181 catcttttac tgttaatcta tacctaagtt cattgaatcc atttaaacca cattttctaa
    12241 ttgctcttga aacggttgct ggagatgaat atgtttcatg agcaatctct actatagata
    12301 attctggtaa tattgattca ttttcattta taaatttaat tatttcaagt tccgtcttcg
    12361 ttagattttc agaattactt ttgtttagct caattatcat atgttatctc cccttatata
    12421 agttttattt gtctaaattt atcgagtaat ataatatatt acctcttaac tatttatttt
    12481 tatatacaat taagatttag tcaatgtcta tatatgtaaa tttataatat cataattttt
    12541 tgcctaaagt ttattatgtt attttatagc attttaaaat aaaaaagaat tgtacctctt
    12601 ccaaaatttg gatgaagtac aatagatatt cctcatactg atatgtttat gattaaaagt
    12661 ataaatataa actttgctat aagtcatata attaataaat cattttattt acaattataa
    12721 ccccccttat aaaactatat taataatata tacagtattt ataagggggg ttatttatat
    12781 ttaaaaacta tttacttttc tcttaattct tctccattgg ttcttattac attttgatac
    12841 caataaaaac tatctttttt acaacgttct ttacttccat ttccattgtt atctatatct
    12901 acatatataa atccgtatct tttttccatt tcttgagatg aacaacttac tatatctatt
    12961 ggtgaccaca tacaataagc taatatttca gttccatcag ctatagcttc acctatttgc
    13021 tctatatgtt tttctaaata atctactcta taactatcat gaacttttcc atcttcaaat
    13081 ttatcatgca taccaaatcc attttcagct ataattattg gcttttggta tctatcataa
    13141 tattgagata aagtattata taatcctttt gggtcaattg cccaccccca aggatttgct
    13201 tttaaatatg gattctttat attatcagtt ggattcatag tgtttttact tgctgatact
    13261 gttgtagaat aatagtatga taaagctaga aaatccatag tattttcttt taagatttta
    13321 tcatcacctt cctccataac tatatttatg ttattttctt tgaaaaatct cttaatatag
    13381 actggatact ctcccctaaa acttacatca gtgaagaaat attgtagcct atttcttttt
    13441 aaagcaaata ctacattttc agggtcacaa tcttctggat aagatgtaca atctgccatc
    13501 atcataccta tcatcatatc tgaatttatt tcttttccaa gcttcttagt caaagctgta
    13561 gctatgaatt gatgatgaat agcttgatat ctagcctctt ctacattatc tacctggtct
    13621 acacaaaatg caactgaatt aaatgattca taatgtatta aatttatttg atttataact
    13681 atccaatatt taactttatc tttgtatcta tttagtatta actctgcata tctaacaaag
    13741 aaatctacca cttttctatt agaccatcct ttatatttta atactaaatt caaaggagtc
    13801 tcataatgaa gtagtgttac aataggttcc ataccattat ctattacttc atttataaga
    13861 ttatcataaa actttaatcc ttcttcatta ggctctaaat catctccatt tggaaatatt
    13921 ctagcccaat ttattgaaaa tctaaaacac tttaacccta gctctttcaa aagctttaaa
    13981 tcttctttgt atgtatgata aaagtctatt ccactacgct taggataata tccattatta
    14041 tctttcatag caaactttat tctttctata gtcatatcag actcataagg tttagatata
    14101 tctctatctt tatcatacat atgtatatca gctaaacacc atcctttgcc accttcattc
    14161 catgcacctt ctaattgatt agctgctaca gctccatccc acaaaaaacc tttaggaaac
    14221 ttacccaatt taatcactcc ttattttatt tatttaaatc atttactatt atttacctta
    14281 tattctatag accttactat tcctactaaa gacatagtta tatttaactc gctgaatatg
    14341 gtcataagag tatcttgtgc atgtgcaaat aacagcgaat gtgctctttt ctctccacca
    14401 gcttcccctt gtataatctt ggtttgagct ctatgtgctt ctgtcataaa ctcttttgct
    14461 ttagctagtt ttaaatcagc atttctataa tcaaactttt ccaaatcgtt aagagcatgt
    14521 ttaatttctg ttctagcctc acctgcacga agtattattt gcattgccac ctctactaat
    14581 tcttcatcta ttatatgttc ttcaattgtc atatttataa tttcctttca atttctaaat
    14641 ttaaatctct tgactctcta attttagctc ttctttctca taagctttaa aaaatggata
    14701 ccatatcata aatagtaata ctatatttat tgctaaaaca ataagacctc taacatcttg
    14761 atttaccata aaagtagata taccaaaggg aacataccat aaattaaaca ccttacttgg
    14821 aattgtaaca aatcccatac tcattgataa ataagtagtt attgatatta ctattgtacc
    14881 tatccacata ggtaacatta aaaatgggtt ccatgctact ggagctccaa atattactgg
    14941 ttcatttata ttcattatag aagggacaat acatgctctt cctattgctt ttattcttcc
    15001 aactcttgat aaacacatca ttataactaa tggtaatgta gcacccaacc ctcctaaagt
    15061 tatccaacca gtttgaataa cttcgtaaac atatatatta gttgctactc ctcctgatgc
    15121 tactgctgct gcattagctg caattgcacc aagttgaatt gaataagtta taggagatag
    15181 tacccatcca cttattccta aagaatatat aaatactact aagaaattta agagtacaaa
    15241 tccaggtaat gtctgtgcta tatctgatac tggtttaaat actctaacta ttatttcaaa
    15301 catatcaaag ttaagtccat aaattaatcc ccaacctaca aacattatca ctgttatagg
    15361 taacatatta tcaaaccact tgcttacaaa atctggaagt gctgaatctt cgccgaaaaa
    15421 tgaaaaagaa ccaaataaag acattattaa tccagaaaat aatcctgcaa ctagtgatac
    15481 aagcattcct tctcctccaa acctgttgaa gttaaatata gctccttcat tcgtaatctc
    15541 tggacgaatc atcattaaca tcaatccagc acctgcaaga ccagctacta cactcatact
    15601 ttcaaatttc ttattcctca ttatttccat aggcaataaa aatgctataa ataatcctaa
    15661 aagtccaaat gtatattgat ttattggact taaatcaggt aaaaagctta tgtaattctt
    15721 caagacagaa actatagtta ctaatgagcc tacaaacacc attggcaaaa ctttcattat
    15781 agttgattga actgattgta cccatatatt cttagtcata ttatttacaa caggagtaaa
    15841 tttttcactt aaaaaatcta taaatttatt cataaacact ctccccataa atattttatt
    15901 tgtttgatat taattctagt gcttcttcca aagcctttgc accatctagt cttccatatg
    15961 cagttttaga tatacatgct actggaacgt tatattcatt agccttttgt tgaatttctt
    16021 ccttcatata atctaaatgt ggtcctagta ataaacaatc tatttctgat aaatacttat
    16081 ctatctctga ctcacttctc gcttttatat ctgcttctat cccctttttc tttgcactct
    16141 ttctcatgct agtcgccata aaacctgtac ttgctcctga accacatgcc aaaagcactc
    16201 ttatattttt cataatgata cctccagtat ttttatccat taatttattt cttaatgaac
    16261 tttttatttt attatgttta tattgtactt accaaataag tttatttcac ataaatatat
    16321 ttcctaataa taggaaatca tttactctta attaaacata ttaatatagt ataatgtatc
    16381 ttataatacg tacattcaat tatataatta tcatatcatc cagattttat tttaaaacta
    16441 tgcgagggaa tctaatgaaa acatctaaaa aagaactatt tttaaatttc ataaaaacac
    16501 agcatgattg gatagattca tctacattgg ctaattatct taatgtatca acgcgttcaa
    16561 ttagaaaata tgtaaacgaa attaattcca atggtgaatt tatattgtcc tctaaaaaag
    16621 gatataaagt aaatctcaat aataattgtc aaaccaaaat tgatagttca gagaacatat
    16681 cacctgataa cagattaaat ttaattttaa aagaacttat agttaatagt aatggaatta
    16741 atatatttga tttaagtgaa gaattatttg ttagcccagc tactattgaa ggggatatag
    16801 tcaaagctaa taaatttata ggaagttata atttaaaaat aaagcaatcg aaatttttat
    16861 tgaaactagt tggaaatgag tctgcaaaac gcaaattaat gagctctata atatttaaag
    16921 aaacgggaag tgatttttta agcttatttg atgtacaaaa aatatatcga gagtat
//
LOCUS       DAEMAE010000068        16968 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEMAE010000068 DAEMAE010000000
VERSION     DAEMAE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.069"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..912)
                     /locus_tag="KPH93_001784"
     CDS             complement(<1..912)
                     /locus_tag="KPH93_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1688609.1"
                     /translation="MIAVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEAY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFKNTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLSKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNHKLSRLISDVSWSEFIRQLEYKANW"
     gene            complement(1562..2359)
                     /locus_tag="KPH93_001785"
                     /pseudo
     CDS             complement(1562..2359)
                     /locus_tag="KPH93_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
     gene            complement(2376..4640)
                     /locus_tag="KPH93_001786"
     CDS             complement(2376..4640)
                     /locus_tag="KPH93_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG1688610.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(4895..5713)
                     /locus_tag="KPH93_001787"
     CDS             complement(4895..5713)
                     /locus_tag="KPH93_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG1688611.1"
                     /translation="MFRINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(5706..6404)
                     /gene="cbiQ"
                     /locus_tag="KPH93_001788"
     CDS             complement(5706..6404)
                     /gene="cbiQ"
                     /locus_tag="KPH93_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG1688612.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLINISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6407..6691)
                     /locus_tag="KPH93_001789"
     CDS             complement(6407..6691)
                     /locus_tag="KPH93_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1688613.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(6681..7433)
                     /locus_tag="KPH93_001790"
     CDS             complement(6681..7433)
                     /locus_tag="KPH93_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG1688614.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGII
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKVLKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(7615..7807)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7939..9711)
                     /locus_tag="KPH93_001791"
     CDS             complement(7939..9711)
                     /locus_tag="KPH93_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG1688615.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDVSEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLINRIRKGYNKDIILGLIQVILNTIVEEKAIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPTLDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVKSVEVNFDTFADFMYEELYSEEE
                     ENIIENDIDSISSSMLVEGVGDFDDSKSSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRSVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLARILIEENAKSRIYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(9725..10654)
                     /locus_tag="KPH93_001792"
     CDS             complement(9725..10654)
                     /locus_tag="KPH93_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG1688616.1"
                     /translation="MDIIDNLRAQGVDEKLIEDVMYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCISAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAVNGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIQFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11044..11232
                     /locus_tag="KPH93_001793"
     CDS             11044..11232
                     /locus_tag="KPH93_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1688617.1"
                     /translation="MEYMHCPEIARKHGISERQVQKLCDKGRIPGVSKFEYVWLIPKD
                     VEKPVDGKTKIRKELQYE"
     gene            11225..11926
                     /locus_tag="KPH93_001794"
     CDS             11225..11926
                     /locus_tag="KPH93_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1688618.1"
                     /translation="MNSVLIIDDDKELCTLMKKCVEQENLSAIIAHSGAEGLHLVDKN
                     KHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKSDEDDKISGLCLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFIADTDCIIVNGMIIDKTNRIVSINNIPIQLTS
                     KEFDLLSFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNRPFY
                     ILTVRGVGYRFNKEA"
     gene            11928..12851
                     /locus_tag="KPH93_001795"
     CDS             11928..12851
                     /locus_tag="KPH93_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1688619.1"
                     /translation="MNTNVYLLLILVLSLIIIAYLVSALIRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEMAINSQSQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGDEKDEYIHVASDKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIIADWIPVLESSHFEYEFDIPEIEYFLHIDANAYTRIINNLLQNIITHS
                     RGDKMTLRIFENKEQAQIVITDNGKGISSDNLPHIFERLYQCDHSRAAKGNGLGLAIA
                     KELINVHKGTITANSIPGMGTEFTILLPKAL"
     gene            13009..13932
                     /locus_tag="KPH93_001796"
     CDS             13009..13932
                     /locus_tag="KPH93_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688620.1"
                     /translation="MNDFVIETKQLTKIYGKQTAVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLASITSGEVDVFGENIKGREKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAVPNAVKRAMEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLILDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDISRASFILECQFNMKDYSVHNDHTLRLYDTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGDGIA"
     gene            13925..14662
                     /locus_tag="KPH93_001797"
     CDS             13925..14662
                     /locus_tag="KPH93_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688621.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIQFYKWSAFSYTLWIILPVVLGILCTMLMYEENKYDMLKQLWIVPISKIGYFFSKF
                     FVVLIYSICFMLTTAVASVLFSVLSGYVVFNWGSILYLLKKCLEIGVITAFVMLPILA
                     IAASQKGYILPVCITLIYVFSGFALMTINMYLHPLSSMGVVIIRNKDIPGITFSQEIN
                     VPLAFLCICIWSIVSLLFANIALGKRK"
     gene            14673..15446
                     /locus_tag="KPH93_001798"
     CDS             14673..15446
                     /locus_tag="KPH93_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688622.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYNGSRYIDNAGWYMDEVQPWATFFVLPAVIALLGSYMICREEDEDTIKSL
                     LLIPINEIKLTIAKMIITFMFSILLYLLLFTITFLTESVLHFSDLSTELVLSCMKEYF
                     LDGIGVFLAISPIIALVSRMKKGYWLALVFTEIYSIAGLFAGMSNVLQTFFPITAIFN
                     LSGYYITTVEKIMGSIISLLICVCISAFILKGLKYNEKN"
     gene            15462..15623
                     /locus_tag="KPH93_001799"
     CDS             15462..15623
                     /locus_tag="KPH93_001799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688623.1"
                     /translation="MKKMKKIKKLIILIVFLFLFGMLHCYTEWKFNTDPPKGIIERIN
                     EWTDKIGLE"
     gene            complement(15989..16597)
                     /locus_tag="KPH93_001800"
     CDS             complement(15989..16597)
                     /locus_tag="KPH93_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG1688624.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVVSSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(16775..16891)
                     /gene="rrf"
                     /locus_tag="KPH93_001801"
     rRNA            complement(16775..16891)
                     /gene="rrf"
                     /locus_tag="KPH93_001801"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5463 a         2893 c         2170 g         6442 t
ORIGIN      
        1 ccaattagcc ttgtattcta attggcgaat aaactctgac catgatacat ctgaaattaa
       61 gcgagatagt ttatgatttc taatcatatt ctttacttgt aaatcttcta tgcaaataat
      121 atcgttctct tttattaatt tagtagatag tttttgtaga aaatcctttc tttgattggc
      181 tatatgttct tgaagtcttg caacttttaa tcttgcttta tttctattta aactaccgat
      241 tgtttttctc gataattctc tttgtaattt agcaagtttg cttaatgact tcttaagata
      301 cttaggattt tctataaagt ctccacaact tgagatacaa aattctttaa ttcctaaatc
      361 taaacctatc tgattattag tatttttaaa tgcttcaata tctacatcag tacagcataa
      421 tgatacataa tatctaccac ttggttcttt agatatagta gcattaagta ttctaccttt
      481 aggtacttgt ttatccctta ctttaaccat accaagttta ggtagtttta tatgttgacc
      541 acaatacata atgtttccat ttgtaaagtt agttttatat gaaaatctat taattttctt
      601 tgatttaaat ttaggaaatc ctgccttttc tttaaagaat tttttatatg cattatctaa
      661 atcttttaag gcgttttgta gtgaaaattt gtcaggttcc ttaagccatt ttaattcttt
      721 ttttaaattg gttaaatcag aactacactg tttataagta aaagtttctt tattgttttt
      781 ataagcttct attcttttag caagatactt attatataca aatctacaac atccaaaagt
      841 tttgttaatt aactcttgtt gttttttatt tggatacatt ctaaacttat aagctttttc
      901 cactgctatc acctcacttt aattgttttt tgatacactt tttaatgatt ttatcatatt
      961 taatatatta tataatgctg tggtgaataa tttattaatc acctataata atgttaacaa
     1021 atgtaattat ttaaaacaat tatttagaag gttttatatt taattttaaa taacatatgt
     1081 aagaaccata agcccttaac ttttatcatt tttgaggttg tcgttcacat aagttcgcta
     1141 ctacctatgc agttctctta tgaacttctt acactttcac gtaagcacag actatatctt
     1201 atccttcggc attatccgct aaggtctacc cacttccact accaatagct tgtagtgtac
     1261 ttccctcaag aggaatagtc gttgaagttt ctcctattcg gagcttacct gctgattgcc
     1321 cattttaaag tacttagggt ttaaccttat accatctaat caattttttc tactttcgta
     1381 actttcacac ttaggtttat ttcatcctta tgttttagtt tgattagctt taggggtttc
     1441 cagcaattca agtagtattg gataggttct atcctatctc tacatatagg tttccctata
     1501 tgctgactat tttagctact aactcatgac taaagtcacg agtgtgcgta gctatttttt
     1561 aatcaaatac agccattaca tttacaccat gttgaccttt agtattaaca agagcattca
     1621 aactagcatg taccattgca agattatctt gtctcccaat agatataaat tcatcattag
     1681 cccctattaa acttcctttt tcaaattcat ataaaaataa atcaaaatct ataatatctt
     1741 ccatatttac atctaagtta gatgctattt gccttataag aaaattatct ttttctaatg
     1801 tatcatttaa cagcccaact aaaggtaaca tatctttttg cttattatat gaatgaccat
     1861 catttatgct tctatttaaa tgtattgcta gatttggtat tatacaaatt ggctttttaa
     1921 agtctataag cctttcattt ggttttaata tactatcacc ttttaaaact actctaccag
     1981 ctatcccaag aggtctatcc aaccatgtac ttaatattgc tcctccatag cattctgtat
     2041 ttaacttcaa ataagatttt tcagcttcta tttctgcatt tggtttaata ctaaatgtag
     2101 gagaatctga atgagagcct attattctaa atccatctct ttctatatct tctgaattta
     2161 ctacaaaagc tactatagct gataaattct tagtaacata atactttcca ccaaccctta
     2221 gattccatct ctgattcaaa actaattctt caaatccatt tttatctaag atttccttag
     2281 tactctcgac tgcatgaaaa gacgacggac tatcatatat gaagtctatt aagtttctag
     2341 caaaatttat actatccatt cctcatttac ctccattata aaatatttag tgttgtatat
     2401 tctttattta tttttttaat tccctgttgg tcaaaagcta tagtaacatc tgtaccaatt
     2461 atagaaacta ctgtcccaac cccaaattta ggatgatgta ctttagaacc aagttttata
     2521 tcatctggat tagtttcttt attagaatct tttattgtag cattaacttt atctgcaact
     2581 ttagatttac ttatagtact catatacttt ttagtatatt tatctaacat attataactt
     2641 gctttagaat agcttagttc tttttttact ttgtacaatc tttctataca ttcttctggc
     2701 aattcttcca taaatcttga agctattgca acatttgttt taccatataa tgttctcttc
     2761 tctgtcaatg acatatatag ctcttcttta gctctagtta taccaacata acaaagtctt
     2821 ctctcttctt ctatttgtga atcactcata gattttatag ctcttgatat aggaaataat
     2881 ccttcttcca tacctgttaa aaacacaaca gggaactcta aacctttaga tgtatgaatt
     2941 gtcataagcg aaaccctatc attgtcttct tcatcatttg attcagatgt aagtgctata
     3001 cttgttagga atgtctctaa gtctttattt tcataagtat catcattact actttcaaac
     3061 tccaaagcta tagatataaa ttctctcaaa ttatcaattc tgtcttgagc ttcttcacct
     3121 ttacctgtta aatctttttc atttttattt cttatctcta caagctcgtc catatatcct
     3181 gtagtgtcca agactttttc tataagttta ctaacaggat aaacttcttt tatagttcta
     3241 agagtcccta taatatcaac aaatccactt atacttgccc ttgctttagt agatatatct
     3301 gaatttgttt ctatatcaat taatactgag tatatacttt cctgttttaa attagctcta
     3361 tcttcaattt tttctatagt tctaagccct atacctcttc taggaacatt tataatcctt
     3421 tttatagata tatcatcttg tggattttga ataactctta aatacgccaa caaatccttt
     3481 atttcttttc tctcatagaa ttttgtacca ccatatatat tgtatggaat ttgacttcta
     3541 tttaaagcgt cctctacagg tcttgcctgt gcatttgctc tataaagtac tgcaaaatcc
     3601 ttataaggtc tattttcttt tctagctatt tgtgcaattg aatcagcaat gaaatcagcc
     3661 tcttctattt cgctaccagt taattgtatc tttataagtt ctccttcttt tttttcactc
     3721 caaagctttt ttctttttct ttctatatta ttagatataa ctttattagc tgcatctaat
     3781 atcacttgtg tagacctata attttgctct aattttacaa catgtacatt gtcataatct
     3841 ttctcaaatt caagtatgtt tcttatatct gcgcctctcc atccataaat actttggtca
     3901 tcatctccaa caacacaaat attttgatgt tctcttgcta ataattttat caattcatat
     3961 tgagctttac ttgtatcttg gtactcatct accattatgt atctaaatct acttctgtaa
     4021 tatgctaaca cttcatcatt agctttgaaa agttctacag ttttaagtat taaatcatca
     4081 aagtcaagtg cactatttct ttttaaacgg tcttgatata acgcataaat atctgcaatc
     4141 ttaaccattc tattatctgc catattgata tctttaaact gctttggagt ataaagtttg
     4201 tcttttgctc ctgaaattgc acttataact gcttttggtt caaacacttt atcacttaaa
     4261 tttaattctt ttagacagtc tttaaccaat gtaacttggt ctgctgaatc ataaattaca
     4321 aaacttctat tgtaaccaat cttatttata tcttttctta gtatacgaac acagcaagaa
     4381 tggaatgtac ttatccacat atccttagtt tcaggaccta tattttgttc aactctttct
     4441 ctcatttcat ttgcagcttt gtttgtaaat gttatagcta atatatttgg tgcttgtaca
     4501 cctttatctt ctataagata tgctattctt gttgttaata ccttagtttt acctgaacca
     4561 gcaccagcca gtataagtac tggaccttca gtcttttcta ctgcttctct ttgagcagga
     4621 tttaatgtat ctaaattcat atttctcctc ctgttaacag ccataaatat attttaatct
     4681 aaaatatgaa ttacttcaaa ttataattat gtattatatt aatagattat ttccaatata
     4741 ttacaattaa ataacatttt tccatataat ttatttcatt gtttaataaa aaaaataacc
     4801 ttatgatttg gggaatcata aggcaccttt aaggggtcta ggttaagggt tattatatat
     4861 aaatctattt ttttaaaata ggtttagggc ttaattattt atttgtatta agttctcttt
     4921 ccttccagta cttatacaaa tcttcttctt tcttaaataa tggtaaattc atatttctat
     4981 gtacttttac taaccatggg gattccaggc cagcttgtat aatattattt tcatttataa
     5041 acacattttc tgcctttcct tcatcaatca aaatcccctt atccaataca tatatgtaat
     5101 cacatatttc atacattaaa ttcatatcat gactggatat tactattttt atattatttt
     5161 tatttaatcc ctttattata tctacaatag acctagtact taccgggtct aaaccagctg
     5221 taggttcatc aagtaataca atttcatttt ccattgctat aactgatgct attgccactc
     5281 tttttttctg tccataacta agaaaatgta ctggcctgtc tataaaatct attccattaa
     5341 cagctactag agctttgttt attctctctt taattgtttt ttcatccata ccaatgtttc
     5401 tcattgcaaa ggctatatca tcatatactt ttgaataaaa aatttgtttt tctgggtctt
     5461 ggaacacaat acctacattt tttctaaggt tatataaact tctcttatca tattttaact
     5521 tttcttcttt aaaaagaatt tctccagatg taggctttaa tatacccatt aaattcataa
     5581 ataatgttga ttttcctgaa ccatttgaac ctattatgcc tataacattt ccctttgaaa
     5641 aatccatatt aatgtttaat agtgcattgg tatttttttc atattgatac gttaaattat
     5701 ttattctaaa catcattatc ccctactatg tgaaatttac catcatacaa ttttatgtct
     5761 aatgatatac acatatcatc ataccttgtc ataagtcttt tgtacagcat acttccaaga
     5821 atacctaagg atttatatga agtttttaaa tttatatacc caaatcttag ctcttgtgac
     5881 ttgtatattt ctgaaaattc ttctaaaaat atgaatataa acctatatat taacattgat
     5941 aactcgacaa aagtatcagg taaatgaagt tttttaaaaa agaatattaa ttgattaaag
     6001 ggtgttgtaa gtatacaaaa atacacacaa gtcaaacaag acatagctct aaataacaca
     6061 tgaatagaca tatcaataga cgcctttgat attcctacat taaaactaaa aaattcaaaa
     6121 ctatataata aatctgcctt attaaaagat atatttataa gagttattcc tatactaaga
     6181 aataaaaagt acattggtat tctaagtaat tttaaataat tttttaaatc tattccagcc
     6241 acacaaacaa ccaaaatact cattaaaatc ataattccac ttaaaataaa tatattttgt
     6301 attaccatag aagcaatcag aaaaattact cctatggcta cttttttgtt tggatttact
     6361 ttacttaatc tatttgtata tgcatattta tcaattataa gcatgattaa cttctctttc
     6421 ctttttgaag acctaatatg tatcctatta caccagcccc taaagctgct tgtgcagaga
     6481 ataataaact ttctatttct ccacttggtg gctcatatgg actgctaaac catggtttgt
     6541 aatttggatt tatcttagta atttcacttt cagcttgacc atctgctcct ccatattcag
     6601 cacctgagtt gacaagtaat gggaatataa taagagctac tactaataaa agtaataata
     6661 tgttagtttt tgtttttgtt ttagcactca ttagctttaa ctcctttatc attattttct
     6721 agtatgcttt tttctccgtt ttctgtaatt aaattatata caattacagt aagaataccc
     6781 tctgctattg ctattggtat ttgagtcata aagaaaattc ctaaaaattt aattgctgaa
     6841 gccattaccc cacctgatgg gtctgggaaa gccagtgcta attgtattga agttattgta
     6901 tatgtggcta agtctcctat tgctgctgca agaaatactg gaatagcatt atttccatct
     6961 tttttcttta aaaccttaaa tattaggaat gatacgattg gccctattat cgccattgaa
     7021 aaagcattag ctcctaaagt agttattcct ccatgtgcta atagtaatgc ttggaatatt
     7081 aaaactatag ttccaaggac aaacattacg cttggtccaa acattattgc tccaagacct
     7141 acaccagtgg gatgtgaaca acttcctgtt actgaaggta tttttagtgc tgataataca
     7201 aacacaaatg ctccacacaa ggccaatagt actttctttt tagggtcttg ctttactatt
     7261 ttacctattg attttaatcc gaataagaaa aatggtataa atataattcc ccatgctata
     7321 gaccatttta ctggtaagta accttccatt atgtgcattg catttgatgt aactggagtt
     7381 aaaatgatta acataagtgc agctataacc cctaatttaa tattctgttt catcttagta
     7441 tccctctctc cctttctaat aattaaaaca taataataca tgtctgattt atttaataaa
     7501 ataaaaaagc ctaactcgat ggttaggctt ctaattttcc acaatctaaa tacaaacaca
     7561 taagacaaac atatctgtat aattattata agaaactttc tccatccccg aagaaattac
     7621 tttaatattt taggcaggtc tcctgactta gcttcttcct actcctcttc cttcccatga
     7681 gatataaaaa atatcatcat agtggctaac aaaagtttcg tctgcatcac agtagcgggg
     7741 gctgtaaagg aatttaacct ttttccctat taatcttgct tgaagaacct ataatattat
     7801 atatttaatt tttaaattat tcttaaatta ttttctatat aaatcattgt ataccttttc
     7861 tgtgaaaatt tctacatttt ttataaattt tattcatttt tatattttta tgtatataaa
     7921 ttcttttttg taagtttatt aatttctaat tatcttcttt aaatacattg aaactacatc
     7981 agaaaatcta gttatatctc ttgaatatat aaaatcattt ccatatatta atctttcagc
     8041 ttcaagttct gattcttttc ctgtaaatac tcccagtact aatatatttt gctttctagc
     8101 ttttcttact tcattggctg tatctctcaa accaactata cccctataag ccttttctcc
     8161 tctcaacgtt ctctcactac tttttcctat ctttacatca tttggtcttc catcgctgag
     8221 aactatcagg attttatttt cttcatctct atttattaga ccactacata tagattttat
     8281 tgccaaacca tctctattat taccagcaca aaaatattca aatatatttt cactattttt
     8341 tatactatca tcataatctt taaatctttt taatattgta tagtctaaaa aactagaaaa
     8401 tcccattact ctatttggta tgccaacttc tgttagagca tttgcaatta tgtaagcttg
     8461 aattgccaca tttccttgat ttctactttg agagccactt gcatccaata atatatcaat
     8521 aacgtatttc cctttttcat tttctatatc tttataaaat attttattat tattacttct
     8581 accaactctc caagctctat ttgctaatat agtaccacaa tcagaatata ttctactttt
     8641 agcattttct tctattaaaa ttctggccag actttctttt aatctcttta tttgctgtct
     8701 atacattcta gcatgttccc taaattttcc taaattattt tctttttgtc ttgttacata
     8761 ttttagttga aatacattat tacactcact tctaagaact ccatctgtaa aatgtattct
     8821 acaaccttca tgaacacttc tacaattttg tgtctcaatt tttttaattt cacctttact
     8881 caaataagtt tttccataat aatgttctat ttttgaatat atcttatttg cagtgtcttc
     8941 atcaacatat ataactcggt ctgctgaatt gctagatttg gaatcatcaa aatcccctac
     9001 accttctaca agcatggatg aagatatcga atctatatca ttttcaataa tattttcttc
     9061 ttcttcacta tagagttctt catacatgaa atcagcaaat gtatcaaaat ttacttctac
     9121 actttttaca ttattttcat caataagact attttcttct ccatttaata tgtattcaaa
     9181 ataagttaaa tatataacat ctaatgcttt taaaatttcc ataatatctt gattggtatc
     9241 tatatttctt atatcattaa ctattcttct agtcatacta tctaatgttg ggtgcttgtc
     9301 catactataa agtacaaatg tgtatttaat tttatccata atattatcat tgtgtatttt
     9361 ggtaaaacgt gaaacaatat ctttataagc atttcttctt atgtcctcta tccctggtct
     9421 atctttaatg gccttttctt caactattgt atttaatatt acttgaatta gacccagtat
     9481 aatatctttg ttgtatcctt tccttattct atttataaga tattttttaa ctatatccca
     9541 atcaacatac tttcttctag cacctgcaat tattgcaaaa tacagaccta tttcctttga
     9601 agaataatct ttttctgata cgtctatact ctcattgtag tcgccagcta ttgtccacga
     9661 taagttattg gctctatttt caaattcata atcatcatat atccatttca taatctcacc
     9721 tatattaaat atgacttatt ggaaacacat ctatactctc ccatttatta ggtattctag
     9781 ttttaattac atctccaacc atttctttct catatacatc aaaagtcttt cctgtaagcc
     9841 ccatattaat tgcaagggtt ggttttaatc ctcttcttat agtcttaaga gatgccataa
     9901 gaccacgtaa atcaatagct ttactagata tctcgccatt ttgagacttc aattgtaaat
     9961 ctaaaaatat tcctgcaaat tgtattaatt tttcttcatc agcatctgaa aattcatttt
    10021 ttaaaatcat cattaactta tcttcttcta taggaggaat atctattacc ataaatctag
    10081 ataccagtgc ttcattaagt tcttttgttc ctgcatattc ataattcata gtcccaataa
    10141 atcttgttgc tggatgtaga ttgactctct catacccagg tacatcaatt attcttctat
    10201 agtctaaagc tgaatgtacc acaactatag catcattttt tgccatatta atttcatcaa
    10261 ataccccaaa acctccatta acagcacact cataaactga acctcttcta agtttaactt
    10321 cattgtctat aaacgtatct gtacctataa gtgttgaact gtctgtattt atatgaaatg
    10381 atgtattcca ctgaggtcta ttaaatatct cacccaaatt atcagcaagt aagttttttc
    10441 ctgttgcctt tggtcctgat aataatatat tttcttcttc taaaatagct gatatacaca
    10501 tagataatat atcttttcca taaaaataag ttttagactt tgttactcta tattctaaat
    10561 ctttttctaa tccataataa tttctaaaat acattacatc ttcaataagc ttttcatcta
    10621 ccccttgtgc ccttagatta tctattatat ccattattct tacccctttt atataaatag
    10681 tatttgtttc atttcttatc accattttag cataaaagga tataagctag aattgatttt
    10741 ttaaactaac ccaccttaaa taagacaatt tatcatttta gcactacaaa aattaataag
    10801 gacgacatga ttaagaagga ttcaataaca aatctacttt tacataagaa tattacacta
    10861 ctgtacaatg caattagcaa gtacaagata aattttgtag atgtgacaat cacattatat
    10921 atgtttatac gttcttacga ttaaccagaa taaagcatat tacttactta tatttcttga
    10981 ttttattcgt atacacatat ataatatatg ctgataaatt ataaagaaaa gtcgattgga
    11041 aatatggaat atatgcattg ccctgaaata gcaagaaaac atggaatttc tgaaagacag
    11101 gtacaaaaac tatgtgacaa aggacgcata ccaggtgttt caaaatttga gtatgtgtgg
    11161 ctaataccaa aagacgtgga aaaacctgtt gatggtaaaa caaaaataag aaaggaacta
    11221 caatatgaat agcgttttga ttatagatga tgacaaagag ctttgtactc ttatgaaaaa
    11281 atgtgtggaa caagaaaatt tatctgctat tattgcacat agtggtgcag agggactaca
    11341 tttagtagat aaaaataaac acacactctc tcttgtaatt ttggacataa tgatgccaga
    11401 tatagatggt tttcaagttc taaaaaaaat tagggaaaca agtaatattc cagtgattat
    11461 gcttaccgct aaaagtgatg aagatgataa aatttccgga ctatgtttag gtgctgatga
    11521 ttatctaaca aaaccattca gcataaacga acttatggct cgtgtcaatt ctcttattcg
    11581 taggtatact atgttaaacc ctgcttttat agcagataca gattgcataa tagtaaatgg
    11641 tatgattatt gataagacaa accgaattgt ttctatcaat aatattccca ttcaacttac
    11701 aagcaaagag tttgatttac tttcttttct ggcttctaat aagggacgtg tatttaccaa
    11761 aaaacagatt tatacacaag tatgggaaga agaatatgct tttgacgata gtaacatcat
    11821 gtctttcatc agtaaattga gaaaaaagat tgaaccagac cctaaccgtc cattttatat
    11881 tttgacagtc cgtggtgttg gttatcgttt taataaggag gcttaacgtg aatacaaatg
    11941 tctatctact tttgatatta gttcttagcc ttattattat agcatacctt gtttctgccc
    12001 tgatacgtgt ccgaatacaa cttacactta taaaagacgc tttagaggat ataaaaaatg
    12061 gaaaccttaa tcgccgtatt ttaacagatg aaaacgacat tacaaaacaa atttgctatg
    12121 atattaacga aatggcaatc aatagtcaat ctcaacttat tcagcaaaaa caatcagaac
    12181 aagcctacaa acagctaatg acaagtctct cccatgatgt aaaaactcca cttgcttctt
    12241 tggtcggtta tttggaagca gtagaaagta agattgttgt aggagatgaa aaagatgagt
    12301 atatccatgt tgcgtctgat aaagctcatt acttaaagaa ttttgtagaa aatctatttg
    12361 aatgggtaaa attggattct aaggaacaag tttttcattt tgatattttt gacttgaatg
    12421 aattatcccg taatattatc gctgattgga tacctgtttt agaaagcagt cattttgaat
    12481 atgaatttga tataccagaa atagagtact tcttacacat agacgcaaat gcatatactc
    12541 gcatcattaa taacctatta caaaatatta ttactcatag taggggtgac aaaatgacat
    12601 tgagaatttt tgaaaacaaa gagcaagctc aaattgtaat aacagacaat gggaaaggca
    12661 tatcttctga caatctccca catatttttg aaaggttgta ccaatgtgac cattcacggg
    12721 ctgcaaaggg gaatggttta ggcttggcga ttgcgaagga acttattaac gttcataagg
    12781 gaactattac cgccaacagc atccctggta tgggaacaga atttacaata ttactcccta
    12841 aagccctgta aaagtacggg gctttcttac tctaaaacaa gataaaagca agattatggc
    12901 aaggttttgg caaggtttat actctataat tctaattgta gttgattttg acgttgtatc
    12961 aactatgatt ttgtggaagc aaatttattg aaagtgagga acttatatat gaatgatttt
    13021 gtgattgaaa caaaacaact tacaaaaatt tatggaaagc aaacagcagt tgacaaagtc
    13081 aatctccatg taaaaaaagg tcgaatctat ggtctgttgg gacgcaatgg agctggaaaa
    13141 accacaatta tgaaaatgat tttagggttg gcttcaatca cgtctggcga ggtagatgta
    13201 tttggagaga atattaaagg tcgtgaaaaa cgagtatatc cacgtatcgg tgcaattatc
    13261 gaaaccccag gcttttatcc caatctgact ggtacagaga atttggagat ttttgcaaaa
    13321 ctacgtggta ctgccgtccc caatgctgta aaaagagcaa tggaaattgt aggcttacct
    13381 tataaaaata aaaaactgtt tagtaaatat tcgcttggca tgaaacagcg acttggtatt
    13441 gccaacgcta tcctacatga cccagagctg ttgattttag atgagccaac caacgggctt
    13501 gaccctattg gtattgcaga gatgagaaag tttattaggg acttgagcgt tgagcacgga
    13561 aagacaatcc ttatttccag tcatattctt tcggaaatct cattgctggc agatgatatt
    13621 ggaattattg accacggcgt tcttttggaa gaaagtagta tgagtgaact ggaaaagaaa
    13681 aacagtaaat atattcttct acaagtttca gatatttcga gagcatcatt tattttagaa
    13741 tgtcaattta acatgaagga ttattctgtt cacaacgacc acaccttacg cctttatgac
    13801 actactctgg atatgggtga aatcaataaa gcacttgtaa tgcagaatgt aactgttatt
    13861 agttctcaac tttgtaatga tactttagag gactacttta aaaaaatcac aggaggagat
    13921 ggcattgctt aaacttattc aaacagaatt tcttaaactg cgtcgtagaa aacttatttg
    13981 gctgatgttg ttgtccgccc tcattatgcc atttttcgca ctcctatact tcaattattt
    14041 tggtaaaaca ggtgttgaac caatacagtt ttataaatgg tctgcattta gttatacact
    14101 ttggattatt ttacccgttg ttttgggcat actttgcacc atgcttatgt atgaagaaaa
    14161 caagtatgat atgctcaaac aactttggat tgtacctatt agtaaaatag gatacttttt
    14221 tagtaaattt ttcgttgttt taatttactc tatttgtttt atgcttacta cagccgttgc
    14281 ttctgtgctc tttagtgtac tttctggata tgtcgtattt aattggggaa gcattttata
    14341 tttgttgaaa aagtgtttag aaattggtgt aatcaccgct tttgtaatgt tgccgatttt
    14401 ggcaatcgct gcatctcaaa agggatacat tctcccagtt tgtatcacat tgatatatgt
    14461 tttttctggc tttgctctaa tgacaataaa tatgtattta catccattat ccagtatggg
    14521 agttgttatc attagaaata aagacattcc aggtattaca ttttcacagg aaattaacgt
    14581 acctttagca tttctttgta tttgcatttg gagtattgta tcattattgt ttgcaaacat
    14641 tgcactagga aaaagaaagt gaggtgtaga taatgtggaa attactttgg gctgaatgtc
    14701 aaaaaattcg tcgttcaaag attgtttgga ttacagtttt tgcatccatt atgatagcag
    14761 tcatagtttt tgcaggaggg caggatgtat ataatgaccc agatttacat tatggcttaa
    14821 agagcgttta taacggttca cggtatatag acaatgcagg ttggtatatg gatgaggtac
    14881 aaccttgggc aacttttttt gttcttcctg ctgtcattgc cctattagga agctatatga
    14941 tttgtcgtga ggaagatgag gacactatta aatccctgct tcttattccc ataaatgaaa
    15001 taaaattaac catagcaaaa atgattatta cttttatgtt cagtatactt ctttatttgt
    15061 tactctttac tataacattt ttgactgaat ccgttttaca tttttctgat ttatcgacag
    15121 aactggtttt aagttgtatg aaagagtatt ttcttgatgg cataggtgta ttccttgcaa
    15181 tttctcccat tattgcctta gtatctcgca tgaaaaaagg atattggctt gcattagtat
    15241 tcacagaaat ttattctatt gctggactgt ttgcgggtat gtcaaatgtt ttacaaactt
    15301 tttttcctat tacagcaata tttaatcttt caggatacta tataaccact gtggaaaaaa
    15361 ttatgggaag tatcataagt ttgttgattt gtgtttgcat ttctgcattt atactgaaag
    15421 ggctaaaata caatgaaaaa aattaaaata ggtatcttaa aatgaaaaag atgaaaaaga
    15481 taaaaaagct gattatacta attgtatttt tgtttctttt tggtatgcta cactgttata
    15541 ctgaatggaa attcaatact gacccaccta aagggataat agaaagaata aatgagtgga
    15601 ctgataagat tggactggag tagtaactta agttaattgt ggtgaatgta atattagcaa
    15661 gttattgttt catattttaa tactataact ttaaatattt ggttttaatc tgccgaacta
    15721 atgtaaaaaa actttcacag aggcaacata tttttgaata tttataaagc ggtagttttg
    15781 attttcaaag ctgccgcatt ttcttttata taagcaaact tactcccaac aaattttaaa
    15841 acatggtaca aaaaaagcac cacagacata agcgataaaa gaaacgccga gaacctacaa
    15901 aaacgtagtg tttatgcaga tttacgacgc tttctatttt ctcaacagtc ttaatgacct
    15961 tgtacaaaag aaatttttct aatatttttt ataatcttat cttgtgatgt tttttatgta
    16021 taaatgactt aataacaaat ccaataaaaa ttatggatat tggtataatt agaactagga
    16081 atttataatc attataatgt gacattattc ttacccaact ctcagaaaaa atatacccta
    16141 tactgattaa tgaagtattc catatggtta ttcccacgat agatgatact acaaaagtta
    16201 taatattttg acctgctata cctgctataa aagatatgta tgttctacat aaagggatta
    16261 atctacatac acaaacagaa atagaagagt atttattaaa ataatcttga gatgcaaata
    16321 ttcctttctt tgcctttgga catatctcta ttattttatt tatgatagat ttacctcccc
    16381 aatttcccac tgtataacaa aatatcgaac caagtatacc tgcaattata cttaaaatta
    16441 aaattgtcaa aaatccaaaa tgatttcttg cagcaattgc tccacataaa ggtaaaacaa
    16501 cttcgctagg taatggaaaa caagcatact caatcataat taatacaaat acagagatta
    16561 atccatattg taaaataaaa ctattgatta cagacactta cttatctatc tcctttctta
    16621 tttaaaggtt ttaagggtac atattctaag gattctcacc ctatatttaa agaatcaatc
    16681 tctttaaact attatatgta tttacacaaa gcatttataa catactttaa aaatagtacc
    16741 ttaaaataag aaacttaata aataaaaaaa gactacgtgg ctacgtccta ctctcccagg
    16801 cagtttccca ccaagtacca tcagcgctag agagcttaac ttctgtgttc ggaatgggaa
    16861 caggtgtatc ctctctgcta ttgtaaccac ataatcttta tgcttaatat ttctattaag
    16921 tttttaataa gtttatagaa tctagtaata ctagtttctt tagagtta
//
LOCUS       DAEMAE010000069        16877 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEMAE010000069 DAEMAE010000000
VERSION     DAEMAE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.070"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            91..312
                     /locus_tag="KPH93_001802"
     CDS             91..312
                     /locus_tag="KPH93_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG1688625.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            312..575
                     /locus_tag="KPH93_001803"
     CDS             312..575
                     /locus_tag="KPH93_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG1688626.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESRYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            637..1209
                     /locus_tag="KPH93_001804"
     CDS             637..1209
                     /locus_tag="KPH93_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1688627.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(1756..2076)
                     /locus_tag="KPH93_001805"
     CDS             complement(1756..2076)
                     /locus_tag="KPH93_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688628.1"
                     /translation="MKNTIVLHYIEKYYCENLILKYLSNSANISKSECSRCFKLSLNI
                     TPYKYFTEFRLLKATQFLKGTNEIISDIAIKVVFHQIIYFSKCFKEKMGYSPKVYRNI
                     KKEI"
     gene            complement(2045..2395)
                     /locus_tag="KPH93_001806"
     CDS             complement(2045..2395)
                     /locus_tag="KPH93_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688629.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVNRITENEQFAIYHFTHKYNW
                     RNEVFSILQRLIRLEKSKNELYMYEALVSISFLWLIMIKNIVLSPIKHKNRINIHMQK
                     ILRYIEKYYCFTLY"
     gene            2715..3248
                     /locus_tag="KPH93_001807"
     CDS             2715..3248
                     /locus_tag="KPH93_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG1688630.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KDLPIFEHTKKWLDIYFSGREPNFTPSLHMVGTPFQTKVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAVGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(4167..4868)
                     /locus_tag="KPH93_001808"
     CDS             complement(4167..4868)
                     /locus_tag="KPH93_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG1688631.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTVNGMQGNINYMQGTI
                     SDMQGSINGIEGTISDMQGSINGIEGTISDMQGNINGIEGTISDMQSNINGIEGTISD
                     MQSNINGIESTISDMQSNINGIEGTISGMQGTISDMQGNIKEVNNRLDNMEPDIKDLK
                     TGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIV
                     TSKNWNDIAKLKSVK"
     gene            5334..5747
                     /locus_tag="KPH93_001809"
     CDS             5334..5747
                     /locus_tag="KPH93_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1688632.1"
                     /translation="MKMPKESIDMIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVRQKGLFYCFECFDFPCKLIKNLEKSYNKRYQASLMENSKFVQQHGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKMK"
     gene            5822..6358
                     /locus_tag="KPH93_001810"
     CDS             5822..6358
                     /locus_tag="KPH93_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG1688633.1"
                     /translation="MIGLKCGMVKLLAHQMIWDKSAKDVITLLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYIERLEQCGIFFRGQDVPKQLLFVMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            6518..8092
                     /locus_tag="KPH93_001811"
     CDS             6518..8092
                     /locus_tag="KPH93_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBG1688634.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     QFDIGNVLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTFGKLLIPFI
                     MAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGSGAPGGIFF
                     PLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTGIILIFEMT
                     GSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGEKILDQFSV
                     MFGSPVANKEIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMTDERDSGAV
                     YDKMENLCKEHTSHIF"
     gene            complement(8764..9369)
                     /locus_tag="KPH93_001812"
     CDS             complement(8764..9369)
                     /locus_tag="KPH93_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBG1688635.1"
                     /translation="MINWNLWRTLNMEYIINPCSGIKIKVTKGQKIEIVDIEGGQVVD
                     FFAEIQDDSTEFISPGVTIDCNESLRLQVGNLMYSNKYKPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINHALGTSHQIIHPINLFMYTKLNTDGSIEVCEPLS
                     KANDKIVLEALENMTLGIAACSVSESSCNSRKCTSIKIVVD"
     gene            9739..10308
                     /locus_tag="KPH93_001813"
     CDS             9739..10308
                     /locus_tag="KPH93_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG1688636.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTNHKECWKNWFKEEQYRLTEILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSKSSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPSVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(10645..11982)
                     /locus_tag="KPH93_001814"
     CDS             complement(10645..11982)
                     /locus_tag="KPH93_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1688637.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVIMILYPIVIGL
                     NFLFINNPLYGELIHSKEYILILSIVQIVSALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDIVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHIKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     VEDIDGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDFSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(12046..12756)
                     /locus_tag="KPH93_001815"
     CDS             complement(12046..12756)
                     /locus_tag="KPH93_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1688638.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLSHVDSVTQTYAKIQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
     gene            complement(13192..15303)
                     /locus_tag="KPH93_001816"
     CDS             complement(13192..15303)
                     /locus_tag="KPH93_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688639.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIICCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SIFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGTIFIILGIFLGYL
                     VPSLTINKLNYRLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKYNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKLIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPDNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(15293..16045)
                     /locus_tag="KPH93_001817"
     CDS             complement(15293..16045)
                     /locus_tag="KPH93_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688640.1"
                     /translation="MLKVNQLCKSYKTGNKTYPILKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGGQVLQMENRAVNLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGGQDDN"
BASE COUNT         5993 a         2081 c         2663 g         6140 t
ORIGIN      
        1 tattaaaact actgattata atatagaacc aatatacaat ttttaaaaat aatgctattt
       61 taaaattcta aaaattaagg aggataatta atgggatatt taaaagagga taaacaagac
      121 tattgttgtg aatttgccag accatacatc aatcgtcaaa tttatgatga cacatacaat
      181 ccaaaaacag cattaaaaag aggtacttta tttccggaat tagatttaat agagtcaaat
      241 aactataatg attggctata tgcaaatcct aaaaatagag ttagagctaa aaaagggggt
      301 aacatgtact aatgtcatta aatagaagag aacttttaga gagaatttca gaatatcaat
      361 ttgcctgtat agaattaaat ttatatttag ataacaatcc aagggataag aaagctcttg
      421 attcatacaa cagatactgc gataaattta cacaagctgt gtgtgattat gaatctagat
      481 atggagcatt aactaatttt ggatatgaat caagtgaata tccttggtca tggatatctg
      541 aaccttggcc atgggacaaa tcattttata aataaaatta ttaagcttta aaattagatt
      601 tattattaaa tatatattaa ggaggagtaa gataatatgt ggatttatca aaaaacactg
      661 gaacatccag ttaacataag acaagcagac cctagaatgg caaaatatat catgactcag
      721 ttgggaggac ctaatggtga gttagcagct gcaactagat atcttcaaca aagatatact
      781 atgccaactg gaaaatctcg cgcactttta actgatatag gtacagagga aatggctcat
      841 gttgagataa tttcttcagt gttatatcaa ttaataggca gttgtactcc agaagagctt
      901 aaggctgctg gtcttggtag taattatgct aattttggac atggtcttca gccagtagat
      961 tctaatggag taaactttac tacaagttat attaatgtct ttggcgattc ggtaactgat
     1021 ttacatgagg atatggctgc tgaacaaaaa gcattggcta cttactatca attaataaat
     1081 ttaactgatg accctgattt aaaagatata ttgagatttt tgggtgaaag ggaagtagtt
     1141 cactatcaaa gatttggtga agcactaatg gatgtttatg agtttacaga gtgcaagcat
     1201 cagttttaat gaagtattaa aagtgaatgg agagtaagca tagttctaat gaagaattaa
     1261 aagtgaaaag agaataaaca taagttctaa tgaagaatta aaagtgaatg gagaataagc
     1321 atagttctaa tgaagaatta aaagtgaaaa gagaataggc ataagttcta atgaagaatt
     1381 gaaagtgaac agagagtaag catcattttt aatgaagaat taaaagtgaa aagagaataa
     1441 gcataagttc taatgaagta ttaaaaatga aaggagaata agcatcattt ttaatgaagt
     1501 atcgaaaata aaaagagtgt aaatattaac tctgataaac tatcaaaaat aagaggtaaa
     1561 ggttttagtt gatttctttt atctcttatt ttttattgat taaacagatt actttgatat
     1621 ttaaatatat tattttagca aaaaaagtcc aattaaaagt tcaaagtatt accatagttc
     1681 aaagcattgc cgttgttggc aagtttttga caatactttt tatttactct ttgaataatc
     1741 aaaagaaata aaaaatcata tctctttttt tatatttctg tacacttttg gagaatatcc
     1801 cattttttct ttaaaacatt tactaaaata gattatttga tgaaaaacaa cttttatagc
     1861 aatgtcactt attatctcat ttgtaccttt tagaaattga gttgctttta ataagcgaaa
     1921 ctcagtaaaa tacttatagg gtgtaatatt tagacttagt ttaaagcatc tggagcactc
     1981 tgatttactg atattagcac tgtttgataa gtatttcaaa attaagtttt cacaatagta
     2041 tttttcaata taatgtaaaa caatagtatt tttcaatata acgtaaaatt ttttgcatat
     2101 ggatattaat tctatttttg tgttttattg gtgacaaaac aatatttttt atcataataa
     2161 gccaaagaaa agaaatagaa actaatgctt catacatata cagttcattt ttactttttt
     2221 ctaaccttat taatcgttgt agaatagaaa atacttcatt gcgccaatta tatttatgtg
     2281 taaaatgata aatagcaaat tgttcgtttt ctgttattct atttactaac gattttactg
     2341 gatttctaac ataaaaatac ttttcctttt tacaacctat tcttactttt cccatttgaa
     2401 agtattatga tattacttga tgttatattg atattttttt gacctattta gtgataaaat
     2461 tataatatac acttctctaa aagttaatac agtaggaagc ttaaatgtat gaattttata
     2521 tccaccaaag ggaaagggag aaaaaaagga tgaacagata caggtaaaga taaaactagt
     2581 atgatatgag gatagatatt ataacaaaag tgtagtcaga aggaaattat attagtatgc
     2641 ttttacataa tggcagatag aaaagaagtg tctaacgatt tgagtgaagt tttcagaata
     2701 taaggaggaa ttgtatgcaa tatattagct attaccatag tccaattgga aatattcttt
     2761 tagcatcaga taatattggg ttaactggtc tttggtttga gaatcaaaag tattatgctt
     2821 ataatcttga ccaagaacat caagaaaaag acttgccaat ttttgagcat acgaagaaat
     2881 ggttagatat ttatttttcg ggaagagagc ccaattttac accatcactc catatggtag
     2941 gtactccttt tcagactaaa gtgtggaaaa ttttacaaca aataccatat ggtaaaacaa
     3001 tcacttatgg agaaattgca tataaagttg ctcaacaaaa aggaatatct aaaatgtcag
     3061 cacaagcagt cggtggtgca gtagggcata atggaatctc tattattgtt ccatgtcatc
     3121 gtgttgtagg aacaaatggg agtttgacag ggtatgctgg tggaattgat agaaaaataa
     3181 aattgttatc tttagaaaaa gtatctgtgc aacattattt tatcccaaag aaagggagtg
     3241 cattataaag ccattaaatt tagaaattct taatcattta tttgatatac aaacatctaa
     3301 ttttgaaagt aataacttgc aattttaaaa aactattctt taaactatat gttatacaac
     3361 cttattccta gtaaacatta tacaatttta aaggtagttt ttagagcttg tattttggaa
     3421 gtttattttc ttttttcatg tgagtagtta tatgtttaga tgcagtaatt tatatgctac
     3481 aaacagggaa ataaggagca gacaaaaaat acttagataa aatgttcaaa atatattttg
     3541 tatattttgg aaatagtaag agtgttaaat ataaatggaa aatttataat gattgatatt
     3601 gaagctactc attaagtatt aagaaccata caagatgagg ttgaaatttg tatgcttcgt
     3661 tttatgtaaa aaatctaagt gagttagaaa tgaaagaatt atttataaaa aatagattaa
     3721 gtattgggaa aatatgaaaa atagatgttg aataaaactt agttgattgg ttaatttaac
     3781 caaacactta aagtattaaa agaaaaaatt attattctta agcaagaagg tataagtgga
     3841 aaaatgaaaa caagatttta tccttttata agtgataagg ggatatgttt taatcaagga
     3901 tggactttta ttttaggtca aaaaattata tagctatatt ataaaatata agggagatac
     3961 acaacttttt agtgttcaat ctcccttact ttttattatt atatcaaggc tcatttagcc
     4021 atgttaattt tagtataaaa tgagtatttt tctaataaat tatttataag taaagtgtgt
     4081 tgtcggataa tcagtatttc tgtataaaat ttggtataac tttaaagaag tatgtgaaat
     4141 tttcaggtat tttttactat ggtatattat ttaactgatt ttaatttagc aatatcattc
     4201 caattcttag atgtgactat ctctatattg tttaaatcat ttttaatatc tgatgtatct
     4261 tctactaatg aagaggtatg tttaatcact gtatctaatg tatttctgtt aatttttaca
     4321 tctgacttaa tttctgtaac atcactctga atagtatcaa gtctactact agtttcaact
     4381 tgtccagttt ttaagtcttt aatgtcaggt tccatattgt ctaacctatt attgacttct
     4441 ttaatattac cttgcatatc gctaatagtg ccttgcatgc cactaatagt gccttcgata
     4501 ccattgatat tgctttgcat atcgctaata gtgctttcga taccattgat attactttgc
     4561 atatcgctaa tagtgccttc gataccattg atattgcttt gcatatcgct aatagtgcct
     4621 tcgataccat taatattgcc ttgcatatcg ctaatagtac cttcaatacc attgatactg
     4681 ccttgcatat cgctaatagt accttcaata ccattgatac tgccttgcat atcgctaata
     4741 gtaccttgca tatagttaat attaccctgc atgccgttaa cagtgccttc gataccactg
     4801 atactacctt gcatattact aatattacct tgcatttcct gtaataactc taatattttt
     4861 tcatccatca ctatcacact cctgttttat tattataaca taatttaggt gttatattcc
     4921 acaaacgcta tgtcatattt aatttttaat catataatta tctgctattc aaaacatggt
     4981 taaatatatc tatattgaga acttttttat taatgaatct tgtgtattgt ctaagtgtct
     5041 aagatactat aataagtgtt tttgaggcat gtgtggttat atttgtattt aaaactgctg
     5101 gtaatatcat atacttttaa atatccctta agttatttat attgattact tgtgtttgaa
     5161 tattttgatt agtagcttac tttttatctt tttttattct tagttttaaa ctttcatatt
     5221 tccttgaaaa tttattggca atgaaagtgc atgaattttt tagctatttt ttatgttaca
     5281 atatgaatat ataacaattc gacaaattgg atttttgaaa ggaataaaat gttatgaaaa
     5341 tgccaaaaga gagtattgat atgattatgt ttgctccttg tggaatgaac tgtatggttt
     5401 gctataagca ttgttatcac aaaaagccat gtgctggttg tttgaacagc gatacaggaa
     5461 agccagaaca ctgccgtaag tgtaaaataa aggattgcgt ccgtcaaaaa gggttgtttt
     5521 attgttttga atgtttcgat tttccttgta aactcataaa aaatcttgaa aaaagttata
     5581 acaagagata tcaagcaagc cttatggaaa atagtaaatt tgttcagcag cacggtttgg
     5641 agaagtttat gcaaaaacag aaggagagat atacttgttc taaatgtgga ggaattatat
     5701 ctattcatga tagggaatgt agcgagtgtc aagaaaaaat gaaatagcta gatcactgtt
     5761 tatcaagatg gaaaaaattg taatgtgtat ataaaggtgg attatattgg ggtggtatat
     5821 agtgataggt ttaaaatgtg gtatggttaa attattggca catcagatga tatgggataa
     5881 aagtgcaaaa gatgtcatta cattattgaa aagtatatgg gataagactg ccattgatat
     5941 tcaacatatt ggaagcactt ctattccaag tattagtgca aagcctatta ttgatattgt
     6001 tgtaggtgtt gcttctctgg aggaagctaa attatacatt gaaaggttag agcaatgtgg
     6061 aatatttttt cgaggtcagg atgtaccaaa acaattactt tttgttatgg gagattttga
     6121 aaaaaatact cgtactcacc atattcatgt tgttgaatgg aatagtgttg catggaataa
     6181 ttatatcaat tttcgagatt atctaaatac ttttacagac aaagcaaaac tctatgatag
     6241 tcataaacaa aaattagcat tacagtttga ggacaatcgt aaaatctata cacagagaaa
     6301 gcaagaacta ataaatattt tgctggaaga agcacgactg tggagaataa atacctaaaa
     6361 taataaattc acatttatta agaaactgaa caatcatttt cttatattta gtaaataaaa
     6421 atctattttt gttgattaaa aaatatctgt acacactcat gactttagtc attggaggtt
     6481 cagattgcgt agaattattg gatgaaggag gaacagtatg aaaaaggata catctcatat
     6541 tatcaaacgg gcagagcact ttcaggtaat tttgattgga gaaggactta tggttggtgc
     6601 tgtagctggt ttgattgtgc ttttatatag gatactttta gggcaggctg gtacttggct
     6661 tagccaaatt ttaaaatttg tagaaggttc tacaataaag ataatagctt ggtttgcagt
     6721 acttgcagtg cttgcgtgga ttgtatcaaa gttggtggac tgggagccaa tgatttctgg
     6781 aagtggtatc cctcagcttg agggggaaat gacagggaaa ataaaacaag tatggtggcg
     6841 agtattgcca acaaaattta ttggaggatt cttatcactt atgggtggtc ttgctttggg
     6901 aagggaaggt ccttcaattc agcttggtgc tatggcaggg aaaggtattt ctagagctct
     6961 tgaccgaggg aagacagagg aaaaatttct gttaacctgt ggagcaagtg ctggattatc
     7021 agcagccttt catgcaccat tggcgggtgt tatgttttca cttgaagaag tccataaaaa
     7081 tttttcagta tctgttttaa tttctgtgat gacagcatct ttgacagcag actatatttc
     7141 agccaatttc ctaggaatgg agtctgtatt ccagtttgat attggaaatg tgttgccaca
     7201 agattattat ggattaatag tgattttagg tgttattctt ggagttatgg gggcatttta
     7261 caattggttt actctatatg tgcagtcttt atacaagaag attcctaaaa ttggtacgtt
     7321 tggaaaactg ttgattccat ttataatggc tggtattctt gggcttgtta tgcctgatgt
     7381 tctagggagt ggacacaatt tagttgaaga acttacaagt catgaatata tttttggaat
     7441 ggttgttttg atttttgtta tacgttttat attttcggct gttagttttg ggtcaggtgc
     7501 accaggggga atattttttc cattgcttgt tttaggagca tttataggtg gaatatttgg
     7561 tatggtaggt gttaaagttt ttggaatgaa tcccgattat gtaaataact ttgtgctttt
     7621 ggctatggca ggatatttta cagctattgt gcgtgctccc ttgactggta tcatattaat
     7681 ttttgaaatg acaggttctg ttagtcaaat gctttcacta tcagtaattt caattgtggc
     7741 atatattgtg gctacactca tgaagtctaa acctatttat gaaagtcttc ttgagaggtt
     7801 acttgaaaaa aatggagaac ctgtacataa agagaggggc gaaaagatac tagaccagtt
     7861 ttctgttatg tttgggtctc ctgttgcgaa caaggaaatt caggatgtac agtggcctga
     7921 aaattgtctt ttggtagcaa ttcaaagagg tggagaagaa attattccta gaggtaagac
     7981 aattcttaga ccaagtgatg taattgtgac tatgacagac gagagagata gtggtgctgt
     8041 gtatgataag atggaaaatt tgtgtaaaga acatacgtca catatttttt agggataatg
     8101 atgaatgttt aataatgata gaatatttaa tgaagatttg attaaaaaat tggtaatttg
     8161 aaagagcgtt aaaggattaa tataagaaaa tgaaaatgtg atatcttaga tgtaaaagag
     8221 tttgaagaaa ataattgtat atatttgtta atgagattat atggactaat tgtatattta
     8281 tacggtacta aaagttgcac tattgtatga ggaatgggaa taaagggttt gaaaaaataa
     8341 gtaaaagact ttttagatat tcttattgtg atattttaat tgcgatattt tgggaagaat
     8401 agaatttatt tataaatgaa aataattaaa aggggttaag gttagatgaa tatcgataag
     8461 gaggtattat tagataatta acttaacatc taacagatag agtttttatg aaaacagtgt
     8521 gtattgcaat aaaaaggtac acgctgtttt ttcgttctgt tacgcaatag aatgctattt
     8581 tttagagtac gagtataaaa cccatattcc cttgtaattg tgtctgaaaa acatcataaa
     8641 atcaagggat ttttacacct aaattcgtaa cataccataa ttaattgagg acgcaagtgc
     8701 ttattctttt gcgccattga tggtattttc tgatttttac atttatttga taaatgaaat
     8761 ttgtcaatca accacaatct taatagaagt acattttctg ctattgcaag aactttctga
     8821 aacactgcac gcagcaattc ctaaagtcat gttctccaat gcttcaagaa caattttatc
     8881 attggctttt gataaaggtt cacatacttc aatacttcca tctgtattga gttttgtata
     8941 cataaataaa ttgatggggt gaatgatttg atgtgaagtt cccagtgcgt gattaatatt
     9001 atcaaaacag tttggatgat tttctccatt gtgataaaaa aaatcgtaca tctcaggtct
     9061 acaacatgga tgcaataagt catgttccct tacatcatca tataaaattt taaacatagg
     9121 tttatactta ttagaataca ttaaatttcc aacttgtaat cttaatgact cattacaatc
     9181 aatcgtaacc cctggagata taaattcagt tgaatcatct tgtatttctg caaaaaaatc
     9241 aacgacctgt cctccttcaa tatccacaat ttcaattttt tgccctttag ttactttaat
     9301 ttttatacca ctacaaggat tgattatata ttccatattt aatgtcctcc ataaattcca
     9361 atttatcatt ctgttctatg ttctcatttt aacacatact ctgtctcttt tctatttgtt
     9421 tcttgacgtt tataacatag tctatcattt tttaattttc ataaaggtgt tgtagtcttt
     9481 tttcaagatt acagcatttt tttgcttaaa tttggcattt tcgcaatatt actgtattta
     9541 gaagtaagaa aaaatattct atatttaaat agatacaaaa gtgtaagaaa ggtgaaaatc
     9601 tgttataata cattaaaaac taaatttaga aaaatttata taattagaaa aatgatttga
     9661 taaaaataaa gttaaatgtt aactaaataa atttgaaaaa gaaaaaatat aaagtacaaa
     9721 tttaaaagga gataactaat gaaaaagaaa ttggaagata tgactttaga ggaattatgg
     9781 aacttgtttc ctatattttt gactaatcat aaagaatgtt ggaaaaattg gtttaaagaa
     9841 gaacaatata gactaaccga aatattgcca atgcaaaaaa ttgttagaat aagtcatatt
     9901 ggaagtactt ctgtgagtac gatttatgcc aagcccattg ttgacatact gattgagata
     9961 attcctgatt ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt
    10021 atgtctaaga gcagtagtag gctttcactc aataaagggt atacagtaga tggatttgaa
    10081 gaacgagttt ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga
    10141 gattacttaa ttgaccaccc aagtgttgca aaggagtatg aaaaacttaa gttggacctt
    10201 tggaaaaagt atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttaga
    10261 gagtttactg aaaaggcaaa ggtattgtat tctaatagat ataattagaa ataggtacaa
    10321 ttagaaattt atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg
    10381 ggttgtttca aaatggagag ataaatccct ttttcattgt ttaaatgata aaaatatatt
    10441 tgatttcata taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacatat
    10501 ttttactaag gtaaataaaa atatcacatt tgattaaatt ttaagttaat gaatatatgt
    10561 gcaaatattt aattataagt tagtacaatt ttacatagta taaaacaata taatttgtat
    10621 tatataagat aatctaattt tagactaaaa taatctaata taaagaaata aagaaaattc
    10681 actctcagta taagagatat ccaattttcc attgtactta ttaacaattt ctctaatatt
    10741 ctcaatacca tatccatgag aattactatc ttttttatct gaaataaata gttcctgttt
    10801 tctattgata ggatttacct tgttgtttgt caataaatag ctaaaatact ccttctcaca
    10861 acgtgccttt aataccactt ttctatttga aaaatcagga attttcaaag atgcttctat
    10921 agcattatct aaagaatttg aaaaaatagt acataaatct ataggctcta tacccaaatg
    10981 attgtctata tctatattaa agtgcacatc aacaccattt tcctccaaac ttgtatgacg
    11041 aagatttaaa attgagttaa gagcttggtt tttgcaaaat tgtttgccac taagagtacc
    11101 tatacctatt tttaactgat taaaatactt tttagctcca tcaatatctt caacttctaa
    11161 atacgattca atcatttgaa agtgattatt catatcatgt cgcattttac gtatttgaat
    11221 ttgttgattt tctagtaagt tatagtattt ttcttttatg tgatttgctt gtttttctat
    11281 cttcatcttt acattttcaa tcaatggttg taaaagaagt atcacaccga tatttgccag
    11341 aattgctgac aagatgatta gataagtgcc ataaggtaag ttgcctaaga taatattctt
    11401 attactaccc atagggatta aattagtagg tggcagaata atagcaatta atattgataa
    11461 aaatgaagat atacatacaa tatcaataaa aatccatatt ttatttgata agtattgttt
    11521 tatattgacc aatctattct ttaggaaaat atgtatcaaa aaccaaaata ataatttaat
    11581 cagagcagaa actatctgga ctattgaaag tattaaaata tactcttttg agtgtataag
    11641 ctcaccgtat agaggattgt ttataaaaag aaaattaagt ccaattacaa taggataaag
    11701 tatcatgata actgatatta taggaattaa tttgcttttg ctacagatta taagtagtat
    11761 aaaatatcca aatagtgtaa ataaaatatt tacagggtcg gaaagccata ttatcatttg
    11821 actaataata gagtagccta aaatcaatat gattttgaaa atccaatgat tcctaaagtc
    11881 tataaaatag gaggtcaact tataaagaaa aaaggctatg aaaatattga ttaatattag
    11941 aataaaattt atgcttgtag agattaaaga ataagtgtcc atttaatata cctttctatt
    12001 aattaagatt ctagaaacta agttttaaaa attaaatctt aaaaattaag attctaacat
    12061 ataccttgta aattttccta caacctcttg atatttttta cggcttatag gaattttttc
    12121 attctgaatt ttggcgtaag tttgagttac actatctaca tggcttagat tgacaatata
    12181 cctttgatgt gtacgtataa aattagaggg aagaacagtt tccatatcat ttaattttcc
    12241 atagtattct aatgttttat caaaggtaac tacagttaat tttcttagct cacttttaaa
    12301 gtagaggata tcttttgtat taattttaca aagattagtt ccaatctgaa ctgtaataaa
    12361 gttttctttc cattgttcta atttttttag tgcttctttt aatactttac atattttttc
    12421 agttttataa ggttttaaga tgtagttcaa agctcctaca tcatacccat gaaatacata
    12481 gtcagaaaaa cctgtgacaa agatgattat agtatctttt gaatgttttc gtatggtttt
    12541 tgctatgtca acgccattac tttttccaag ttcaatatct aaaaaaataa tatctatgta
    12601 ataattttcg tgttctagat tgtatactag gctttctcca tttgaatatt cttttatttg
    12661 gtaggggtaa ccccacaaat ccatttctct tgtaatgatt ttattcagca tgtttctttg
    12721 ttgtttttca tcatcacaaa tacaaatcga agtcaatttt ttatctcctt tcaagtttta
    12781 tatttatata gttaagtata taatatattt tgctacctag atatataaaa tgaaataaga
    12841 agtatatcca gatagcaaaa taaaattttt tatattagta tacttacact taaaactatt
    12901 ctattaaact tattctgaag tgccaatgat tttcttatta aaaaatctaa taacctttct
    12961 tattataaaa taccaatgat tctcttactt taaaaatgta ataacttttc atattatgaa
    13021 ataccaatga ttttcttatt ataaaaatgc aatggctttt tacattatga aatatcaatg
    13081 atttttttat tataaagtgt caatgatttt attactttaa aaatgcaatg acttttctta
    13141 ttataaaata ccaatgattt tattacttta aaaatctaat aacctttctt attataaaat
    13201 accaataatt tttttgaatt tcttataagt taatagatat ataaggtaca tataaacccc
    13261 acaaatccct ataaaaccta agtttatcaa aagagcatta acctcactat tgacgattct
    13321 accatcatta tttaacataa tcataaaatt aaacagataa attaaaaaac ttcctgcaat
    13381 aagagggtat gaaaatactt tctttaattg atttctaata cagaatttta tataatcgtt
    13441 gtttgctcct aatcgattta aatccataaa aacctgcttg tgatttatag ctatagtaac
    13501 cccccttgta tatgcaatga ttcctactgc tgtaaaacat atgattgcca catatatgaa
    13561 taacattaag aatactgcca tgttttttat catatcctgg ctttgtaaaa ttttaaagtt
    13621 aggatagtat ttccaattaa ggtacagctg attgttatct gggtctagtt ttaaatgacc
    13681 ttcaccactt ggatattctt catccataaa atattttctt ccagttgatt cataacgttt
    13741 ttttacgtat tcatcataac cactgaacat gcccatttcc ttagatgttc tttttataat
    13801 ttctttcttt aagtcattag caaaagcata ggtatcttta tctacattaa atagtataga
    13861 attattgtaa ttttttatgg ataaatcttt agtaaatgat ttataatcct catcattaag
    13921 gacataggcg ttcatacttc tattttgaaa aaaagaacca ttaaatatta gttttccagc
    13981 atatctaaag gggatttctt tttctgtgac tgggcttgat ataaatttgg tatatttata
    14041 atcttgattt tcatttcctt tatttacaac agcaagatat tcaccatttt ttatagttat
    14101 tggtttgcca attagcttgg aaaaatcttt agaattaaag aacatatcat atgagctttt
    14161 tttcatatat accttttcga tagttccatc tttatgatat attgattcta caccatcaac
    14221 aattaattct atagaaggaa tttccatata gttttgaatt tttatattgt atttatctgc
    14281 taattgttca atatcttttt ttgatacctg tttatctctt tgattgtaag taaaagtata
    14341 atctataggg ttatttttaa gagtatcata aatacctgta accatattag gaacatagaa
    14401 tacagcaaat aatgctcctg caatcaaaaa tgaaataaca cacatgtttt ttactgtttg
    14461 tcttcccata aatctcatca tgctagttga tatgatgttt ttatagtatt tctctggatg
    14521 ttttcctctt ttagcatgta caactatgta tgtcataatc atatatattc caataagaga
    14581 gagtagataa gtaaagctcc atatagatgg cagtctataa tttaatttgt ttatagttag
    14641 tgaaggtacg agatatccta gaaaaatacc gagtataata aaaattgtac ctaatatacc
    14701 ataccagtcc tttacatcct gtattggttc agatattctc tggtcattta atatatcaat
    14761 tatatttgta cgattaataa attttacaga aagtataaat atacataatg ttacaaatat
    14821 actaaatata aatccatata tgaatccttg agttccaaaa tgatatgtca tttctctagt
    14881 atcaataatc actaattgaa atattttcca tataccaaaa gatacaggaa tacttaatat
    14941 aagacctaaa aaacagcata ttattgaaat taacaatatc tctgaaaata gaagtttttt
    15001 taatttcttc ttatctgttc ctagagccat gaaaatacca atttccttgc ttttaaattt
    15061 taaaaagagg gaagaagcgt atgtagtaaa gacagcacat cctaatatag caactgagaa
    15121 tatcattgta gcctgttttc tgctatctcc accagttgga agaatgtttt gtattgttgg
    15181 gctaaaataa attagagcat atgaagttac taataccaca gaaagtatta tacaaaagaa
    15241 taaagttgta tagttcttcc aattataggc tcgaagttta ctgtataatt tattaattgt
    15301 catcttgacc gcctcccatt tcttttaaaa cttctagtaa ttcatctagg aatttatgtc
    15361 ttgaatttga ttttacaact tctttataaa ttattccatc tttaagaaca ataactcggt
    15421 cgcagaagct tgctgcaaag ctatcgtgtg taaccatgag tatagtagca tctaattctt
    15481 gctttgcttt taaaaatgat tcaatgactg tacgacaaga cttactatca aggttaccag
    15541 ttggttcatc tgctaatatt aagtctgggt tgttcatgag tgctcttgct actgctgtac
    15601 gctgtttttc tccaccagat atttcggcag ggtatttttt tctgatatgt tcaatgccaa
    15661 atagctcaca tagattaact gcacggtttt ccatttgtaa gacttgacca cctttgataa
    15721 tttgtggaac acatatattt tcaaagacac taagaccatc aaatagcatg aagtcttgaa
    15781 atacaaatcc tagttgttgg tttctaactt ctgaaagttc cttttctttt aaatttctta
    15841 tattattatt tccaagaatt atttctccat tatcgattgg gataaaacat gaaatacagt
    15901 ttaaaagagt agtttttcca cttcctgatg aacccatgat tgcgacaaat tctcctttgt
    15961 tgatttcaaa agaaatattt tttaatatag gatatgtttt attgcctgtt ttgtatgatt
    16021 tacataattg gtttacttta agcatatata tcatcctcac tttcatttat cttgtaagtc
    16081 aatgatagca tttgaaaggg tggagggata tgagctacat tgtaatagtt ggatgttttt
    16141 ttgtaatgtt tgaaaatata ttgtttgaat agtgtgagag gttattatag ttaaattgat
    16201 tatattatta ggggatttag aaggttattt taggtattaa ttgttgtggt aaagtaatat
    16261 gatgtggaac acattgaagg tgtagttaga aaagaggagt atagtaaaag taaaaaggtt
    16321 atagaataat gagtgttgta ttgcaaaaaa atgatgtaaa taatgtagag taaggttaaa
    16381 aagaaaaaat ggaataaaat gagttataga gcagtaagat tatttaatgg aatgtattgt
    16441 aaataatttg aaaggattta ggggttatta acaccatgta attgagaaaa tatattggag
    16501 ttttgaaaat aaataggttg ttaaaaacaa ttgaaatgta taataaaaag gttaaaaagt
    16561 attattagta taagtatgat agataatttt agaaagtgta ggtatttaag aatatgctga
    16621 aaagtgattg atgtgatttt gaaatattaa aatattacta tattatagta atgaagttaa
    16681 aataataaaa tgctgtaata ataaaagtct gcagtataat aaagcactgt gataaataag
    16741 agcttgtaat ataataaaaa tattgtaata ataaaagtct gtaatataac aaaacactgt
    16801 aataaataag agcttgtaat ataataaagt tgtaataata aaagtctgta ataaataaga
    16861 gcttgtaata taataaa
//
LOCUS       DAEMAE010000070        16813 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEMAE010000070 DAEMAE010000000
VERSION     DAEMAE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.071"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(394..597)
                     /locus_tag="KPH93_001818"
                     /pseudo
     CDS             complement(394..597)
                     /locus_tag="KPH93_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(597..926)
                     /locus_tag="KPH93_001819"
     CDS             complement(597..926)
                     /locus_tag="KPH93_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG1688641.1"
                     /translation="MPQIKIRGINENDICKISEKMIDDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            1256..2182
                     /gene="fba"
                     /locus_tag="KPH93_001820"
     CDS             1256..2182
                     /gene="fba"
                     /locus_tag="KPH93_001820"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG1688642.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(2317..3948)
                     /locus_tag="KPH93_001821"
     CDS             complement(2317..3948)
                     /locus_tag="KPH93_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688643.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIVYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDR
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4431..5468)
                     /locus_tag="KPH93_001822"
     CDS             complement(4431..5468)
                     /locus_tag="KPH93_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG1688644.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5487..6272)
                     /locus_tag="KPH93_001823"
     CDS             complement(5487..6272)
                     /locus_tag="KPH93_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1688645.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(6305..7438)
                     /locus_tag="KPH93_001824"
     CDS             complement(6305..7438)
                     /locus_tag="KPH93_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG1688646.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7486..8613)
                     /gene="hadC"
                     /locus_tag="KPH93_001825"
     CDS             complement(7486..8613)
                     /gene="hadC"
                     /locus_tag="KPH93_001825"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG1688647.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8613..9839)
                     /gene="hadB"
                     /locus_tag="KPH93_001826"
     CDS             complement(8613..9839)
                     /gene="hadB"
                     /locus_tag="KPH93_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG1688648.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9854..10654)
                     /gene="hadI"
                     /locus_tag="KPH93_001827"
     CDS             complement(9854..10654)
                     /gene="hadI"
                     /locus_tag="KPH93_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG1688649.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10693..11892)
                     /gene="hadA"
                     /locus_tag="KPH93_001828"
     CDS             complement(10693..11892)
                     /gene="hadA"
                     /locus_tag="KPH93_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG1688650.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12570..13568
                     /locus_tag="KPH93_001829"
     CDS             12570..13568
                     /locus_tag="KPH93_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG1688651.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLIKTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13930..14457
                     /locus_tag="KPH93_001830"
     CDS             13930..14457
                     /locus_tag="KPH93_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG1688652.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14484..15080
                     /locus_tag="KPH93_001831"
     CDS             14484..15080
                     /locus_tag="KPH93_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG1688653.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDAYDEIVFCGYGEPLMRIDELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARYCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(15145..16700)
                     /locus_tag="KPH93_001832"
                     /pseudo
     CDS             complement(15145..16700)
                     /locus_tag="KPH93_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
BASE COUNT         5158 a         3027 c         2146 g         6482 t
ORIGIN      
        1 cttacacttt catgtaagca cagactatat cttatccttc ggaattatcc gctaaggtct
       61 acccacttcc actaccaata gcttgtagtg tacttccctc aagaggaata gtcgttgaag
      121 tttctcctat tcggagctta cctgctgatt gcccatttta aagtacttag gatttaacct
      181 tataccatct aaccaatttt ttctactttc gtaactttca cacttaggtt tatttcatcc
      241 ttatgtttta gtttggttag ctttaggggt ttccagcaat tcaagtagta ttggataggt
      301 gttatcctat ctctacatat aagtttccct atatgctgac taatttatct actaactcat
      361 gactaaagtc acaagtgtgc gtagctattt tttaatcaaa tatatatatt ctctatatat
      421 aacttcttct ccattaaaca atatctcatc attttcagtg tctacttgag ttcctggatt
      481 tgaaacttca cttctattta ctactacaga cccttgttta caatacttct ttatttcact
      541 tctacttcca taacctaaat ttgaaagaat tttatctata cgttgctttt tagccattaa
      601 aaatgctctc cattctcata atatttttct ttttcaaata ctgtaaatgc taaatccata
      661 ctttctacat ctaaattatt tcttatacta tcagttatta ttcttgctac tatatcttgt
      721 acttcttgcc ctctatcaaa ccaagctact tcaacaaaag gatatacatc tgctatttta
      781 ccatctctta ttgcaactga ctttatacat tctatttcaa agtagtctct tggacatttt
      841 acagcttcaa ctaaatcatc tatcattttt tcacttattt tacatatatc attttcatta
      901 attcctctta tttttatctg tggcatagtt tacctccttt attttaaatt tttagtgaat
      961 tatcatccac ttaattccct tcgataacgt ttgaagtggt agcttcttgg tcaagacaac
     1021 tattgttatc agtatctcca aactaataag gtagttccta cccttatagt tagcattata
     1081 tagctaagtt tcctaaattg tttatattct ttattatttt atttaatcct attgtcttct
     1141 atttaacatt atacttgtaa tttatagtta ttacaattta ctatatactt tttttgattt
     1201 aacgttataa tataagtgat gggtaaattt taaaagttaa taggaggagt ttaaaatggc
     1261 attaattact acaaaagaaa tgtttaaaaa agcttacgaa ggtggatttg ctataggtgc
     1321 atttaacata agtgacttag aacaattaca aggtgtttta aaagctgcta aagctaaaaa
     1381 ttcttatgtt atgatacaag cttctatgtc agctgttaaa tatgctggac ctcatacatt
     1441 agttgaaatg gttaaggctg cttctgatga aataggtgta gatgtagctc ttcacttaga
     1501 ccatggtcca aatatggatg ctattaaaac ttgtattgat gctggtttct cttcagttat
     1561 gatagatggt tctcattttg actttgaaga aaatgttaga ataactaaag aagctgttga
     1621 atatgcacac tctaaaggtg ttgttgttga agcagaactt ggtgttctag ctggaacaga
     1681 agatgatgtt acttctgatg ttcacaagta tactcaacca gctgaggcag ttgagttcgt
     1741 tgaaagaaca ggtgttgatt cattagcaat agctattgga acttctcatg gtgctttcaa
     1801 attcaaagga gaagctaaat taagattcga tatactagaa gaaatccaaa gcaaattacc
     1861 aggtttccca atagttcttc atggtgcatc tgctgttgac caaaacgctg ttgctacatg
     1921 taatgaattt ggtggaaata tagctggtgc taaaggtgta cctgtagata tgttaagaaa
     1981 ggcatcttct atggctgttt gtaaaataaa catggatact gacttaagac tagctatgac
     2041 tgctgctatc agaaaattct tagctgaaaa tccaaaagaa tttgacccaa gaaaatacat
     2101 tggtgctggt agagatgcta tacaagctgt agttgaaagc aagatagatg atgttttagg
     2161 ttctgctaac tctataaact aatataactt ttaaatattt ataaaagaaa agagatgact
     2221 taaagtaatt aaatttattt ttaagccatc tcttttaagt tgtatcaatt atgtatctta
     2281 taatatttta attatattgt ctttttaaac ttacagctaa acgtgaatat gtccactctt
     2341 tatctttcta tggatggtag aaggatatat acctataact tcagcagcct ttgttattga
     2401 accatactta tttatagcct tgcttatcat tatttggtcc actattttat atgcatcatt
     2461 aagattcata attccattta ccactattgg tgataaatca tctgtttcat tatcaatcat
     2521 atctaaattt atcagcatgt taaattcatc ttctccaatt acatttttag ctgataatac
     2581 tacaaatctt tcaatgatgt tttttaattc tcttatgttt cctggccaag aatattcttc
     2641 taataactca ataactttag gtgaaacttc tacttctcta ttgtactttt cattgtaaag
     2701 ttttaagaaa taatttacta atggtactat atcctctttt ctttctctaa gaggaggtat
     2761 ttttactgga ataacactta atctatagta caaatcttgt ctaaatttaa ggttatttct
     2821 aaggtcttca tgagaaatat tagtagcact tatatatcta acatctattt ttataggctt
     2881 actaccacct attctagtaa tagtattttc ttgtataacc ctaagtaatt ttttttgcat
     2941 ctgcattggt aactcaccta tctcatctaa gaaaatagtt ccaccatttg cctcttcaaa
     3001 aagtcccttt ttaccttttt tcttagctcc agtaaaagaa ccttcttcat atccaaaaaa
     3061 ctctgactct aataattcat ttggtatagc tccacagttt atagctataa aaggtttgtc
     3121 tctccttgaa ctatacttat atattgttct tgctattatt tcttttccta cacctgattc
     3181 tccatttata aatatagaag aatctgaggc tgcagctctc ttaacaactt tttttatatt
     3241 gtccataatc ttactttgac ttatatactc tgtgtcttct gatatatcac tatattttat
     3301 ctgagattca agtttaataa cccttgaaat atctcttcct gttagtactc ctcctgtaag
     3361 ttttccctta gaatcccatc ttggaactgc tgtatatgca ataagcttcc catctgggta
     3421 aacgatattt ctataaatct ttttcttcac tttaagcatg tttaatatta ttggttcttc
     3481 aactaatcct tgtctcacaa ggtctgttat atttctacca agtacctcag ttcttttaaa
     3541 aggtaatatc tcatcactga cagtattcat cttttcaatt accccatctt tgttaaacac
     3601 aacaacaact tcatctatat gctctattat atcttcaatt actctatcag atattagaga
     3661 cttctcaaat aaataaacta tattattttc attattatta tctttcatat tgtatatatg
     3721 tgcagtcatc cttaactctc tgaaagctat tttcttttta ctatctgtca gtatttctgt
     3781 tttgtcaaaa tttgaatcta attcatttat atttctaggc ttttttatat ctagaaaact
     3841 aaaaatacta tctattagat gattacagta tactatttcg cctgattctt tacataaaat
     3901 ggctggattt tctacatgtt ctataagccc tattaaatta ttatccatta atattcgcac
     3961 ctttcattaa gtaatttatt gaactacata caaaatttat tgtttgctta caataaatac
     4021 catcagcaca tataatcaac tccttataat attcgtcttt tcaagtagtt tatttcatta
     4081 aaacgtaact aagatataat tttaatgatt atagtttgtt aaaaatttca ttttgctttt
     4141 aattgtattt tatcacatat ttagtaatta tttgtctatt taatacaatt taattaaatt
     4201 taagatttat ttaaaaaaac aaagataatc cttacagaat ataactcaca atttgtaagc
     4261 aatatacata taaggattat ccagattatc ttgtattata aaattttatt caaacagact
     4321 ttttaataac tgacaaatac acactagcaa taacttatca aagggttaat aattatgtta
     4381 ctaactaata agacttattc aatcagtaat atttatatag tttgtatcta ttaagctttt
     4441 atagcttcta ataaatcatc aacactatct aatttttcta taagcattgg aactatttca
     4501 tgtaagtctc caactactcc atagtcagcg atttctaaaa ttggagctgc tgggtttttg
     4561 tttatagcaa ttatgaaatc tgatgattgc atacctgcta aatgttgtat tgctcctgat
     4621 ataccacaag ctatatatag gtttggttta acagttgttc ctgtttgacc tacttggtga
     4681 gaatggtcta tccatccagc atcaacagcc gcacgagaag aaccaactac tccgcctaat
     4741 ttgtcagcta atttcttaag catttcaaat ccttctggtc cacctaatcc taaaccacct
     4801 gatactataa catttgcatc tgtaagagat actaaatctt tcttagtttt aactgtttct
     4861 aaaacagtag ttcttatatc atcttgagtt attttgtagt ctaatgctat aacttcacca
     4921 gttcttgttt cgtctttttc agctttatcc ataacaccag gtctaactgt agacatttga
     4981 ggtctatggt ttggacaaat gattgtagcc attatgtttc caccaaatgc tggacgagtt
     5041 tgttttattt tcttatcttc tgggtctatt tctaacttag tacagtcagc tgttaatcca
     5101 gttccaactc ttgaagctat tctaggtgct aagtctctac ctatatgagt tgctccgaaa
     5161 agcattattt ctggttttat ttcatctatt gcatctttta ttacttttgt atatgcatca
     5221 gttgtatatt tttctaaaag agcatcatct gcaacataaa ctttatcagc tccaaaagtg
     5281 attaattctt tagctaatcc atcaacatct tttcctaata atattgcaca aagttctgca
     5341 tctacttctt tagctaattt tcttccttct cctaataatt ctataactac tggagctatt
     5401 tttccttctc tttgttctgc aaatatccat acgtttttat aagaacttaa atcttttata
     5461 tcattcatag tttgtactcc tccaacttat ataatgtgct tttgttttag accgattatt
     5521 attttactag ctatttcatc agctttacct tctaagattt ctccttttcc tcttggttca
     5581 ggagtaaatg atctaaatac tttagttggt gatgctttta aaccaacttc ttctttattt
     5641 acatctaaat catcaattgt ccaaactttt acatcatgtt tatatgcttt tactatttta
     5701 tcaacagaca tgtatctagg tgtatttaac tcttttactg ctgttaaaag tacaggtgta
     5761 cttactttta ttaattcgta tccatcttct aattgtcttt gaactattat atcttttcct
     5821 tctattttga aatcttgtac atatgtaact tgaggaatgt ctaatttctc tgcaatttgt
     5881 ggtccaactt gtgcagtatc tccatctatt gcttgtcttc ctgcaaatat tatgtcatag
     5941 tcacctactt tagatattcc cgctgcaata gtatttgatg tagcccaagt atcagctcct
     6001 ccaaatgctc tatcacttaa taatattgca tcatcagcac ccatagctaa acactctctt
     6061 agcataaagt ctgcttgagg aggtcccatt gttataactg ttatagttac atcatcatgc
     6121 tcatctttaa tttttaaagc ttcctctaat gcatttgcat catctgggtt taatatactt
     6181 ggaactccat ctcttataag agtacctgtt tctttattta ttcttacttc gttagtatct
     6241 ggaacttgtt ttacgcaaac taatattttc aaagtctata cctccaattt cattagatta
     6301 tttattattt taatacgttt gatgaaatta ccattttttg aacttctgat gttccttcgt
     6361 atattgatac gattctagca tctctgtaca ttctttcgat ttcataatct tttataaatc
     6421 catatccacc gtgaagttgt aatgctttat aagcaacttc atttgcaatt tctgatgcat
     6481 agtatttagc catagcagat tcttgagcaa ctctcattcc tgcatctctc ttttgtgctg
     6541 catcataaac taatcctcta gctgcacaaa cttttgtttc catatcagct atagtaaatt
     6601 gagtattttg gaatttagct ataggcttac caaattgaac tctttgtttt gtatatttaa
     6661 cagcttcatc taaagcacct tgagctattc ctagagctaa ggcagcaaca cctattctac
     6721 caacttctag agtacccatt gctatcttaa atccttgacc ttcttttcca agtaagtttt
     6781 cttttggaac tttaacgttt tcaaatatta agtcagaagt ttcagttcct cttataccca
     6841 tcttatcttc atgagctcct gttgagaaac catcccattt actttctact ataaacgctg
     6901 atgttcctct aagtccttta cttctatctg taactgctat tactatagct acatcacaga
     6961 atggtccatt agttatgaaa gtttttctac catttaatat atagtggtct ccaactaatt
     7021 ctgctgttgt ttgttgtcca cctgcatcag aaccagctcc tggctcagtt aaaccaaatg
     7081 ctcctattaa ttcacctgtt gctatacctt ttaagtattt ttgtttttgc tcttcagtac
     7141 cattgtaaag tattggtaat gaagcaagag aagaaccagc tgtaacaaaa gtagctgttg
     7201 aagcacatct tctagctatt tcttccatta ctatagcgta agaaacatat cctgctcccc
     7261 caccaccata ttcttctggg atattactgc ttataaattt tgcatcagct aattttttga
     7321 ttaattcctt aggcattaca cctgttttat ccatttctgc tggtaaagta tctaattctt
     7381 tactaacaaa atctcttact gcttttctta aaagttcttg ttctttgtta tataacatag
     7441 cttcctccaa ttttcatatt ttatagtaaa tttattttaa tgaacttacg ctaaactcat
     7501 catctcagca aaagtttgaa tacgagttct ggcttgttca ttattctgag tttgttggtc
     7561 gatttcaaca tataaagtag gtattccact atcttctata tcttttctaa ctaaaggata
     7621 atcgtattct tctgggtcac agaatttcat catacagaag ataacaccat ctatatcttt
     7681 ctttttaact tcatctacta taagtgaccc acgttttttc ttagggtcat aagctaatga
     7741 acatccttct atgtttgacc attgtcttgc taatctctct aacgcatcat cacctgctgg
     7801 tacatctgtt ctgaattgtc ttgtttcttg tgctaagtcg tcagctacaa ctgatatatt
     7861 gttgtcttct aaaatgtcta atatatcttt agaatcagct aatatacctg ttaataaaac
     7921 tttctttcca gaacagactt cttctggcat agcatttaat tttgctatta aatctttcac
     7981 tagctcagta tgttcttctt ttctcataaa gaatccactc ttaattacta agcttcttat
     8041 tgatggtttt atagtattag aatgtttata agctacttca acaaagtctc tcatagtttt
     8101 tctatgttca ttgtaaactt ctatactttc atgaattttt gcttcttcta tttcatatcc
     8161 acaaatttct tcaagttctc tttttacgcc tttgtactca ctgattaagt attttacacc
     8221 agcttcaagt tttctatttt gtgggtgtac taatgatata tattttatat gaggtactgc
     8281 tgatttccag ttttgtccta aacaaattag tgtatcacac atccctggta taataactcc
     8341 agataattca tcataagcac cttttaatcc atattctaaa catgattgca ttattgaaca
     8401 tgcaaatgca gggaaatatt gtttagctaa atctaattct gtttgtcctc cccatatacc
     8461 tactggaagc attccagctg catgtataat ttcttctggg caataaactg ggaaacaacc
     8521 tatagctttt ttaccagttt cacttttata tttttttaca gccgcatttg gattttcaac
     8581 tacttctttc attttagata aaatagcttc cattatatct cacctctatt aagttttttt
     8641 ctttcttcca ttacttcaac taaaccttga attcttgttt caaattgagc attagtgaaa
     8701 gctctagggt ctgcttggtc accatcaaat ccagcatatg gtaatccagt ttcttcagct
     8761 gctcttcttt gcatttcata ttgtattaaa ctcataagtt tacagcttct gttcatatga
     8821 tagaatgctc catcacattt accctctact aaagaatcaa ctctatattt tgtcatacgg
     8881 tctaggttta cattgttaaa catagtacta tatgctactg ccattccatc taaatcatta
     8941 acttcatatt gtaatgccca agcatgtggg taaacactac ctgtcatgtt aactccaaat
     9001 ttagctaatg ttttcatctt gtatcctata tatggccaac aaggtatacc ttccatcatt
     9061 attctgtatt tttcttcccc tctgaaagaa gatttaccag ttttcatgtt gtcttctaat
     9121 tcttctataa gtaacttaaa tgcttctgta gtttcttttt tacctctagc acaaactatt
     9181 acagccatgt aagtaaataa gtcaaatcca ttcattggag aaggagaaga atctgctggt
     9241 aaactcatag aatacttcca tagtcttccg ttttcagctg atattttcat tacttcttca
     9301 aacttcttag ggtcaaattt ctttcctgat ataatttcta gttgttttat agcttcttca
     9361 aattgagctt taatataatc tattcttgat tgagtaactt cgtcttcatt attgaaagtt
     9421 gtatcaatca ttattaaagg tatatctaat tctcttgaaa tattttcata ccattttata
     9481 acttggttac atatattgtt acagcaaagt aggaaatctg gagctggcat atccaaagct
     9541 tcacatccac cattttctaa aagaccaaaa tttgttcttg catatgcaca taggtcaata
     9601 gaatatcctt tagattcagc tatttcacat aattctaaag aacctttttt agctgctact
     9661 ccagctgctt ggttttctgg atataatacg tttaagtcaa atacttctgc taactcttga
     9721 ggaaatactg atgttgacca acctacaggt cttccttctt ctttagcttt aaatgcattt
     9781 gcatattgtt cagctaataa atcattaatt actactctag cttctttttt ttcagacatt
     9841 ttcatccctc tttttatata tttttcactt ctttttgtga ttcttttgct tcatcaaaag
     9901 catataacgc tgctcctaaa gcaccagtta attgaggtat atcaggtaca ataatttctg
     9961 tgttgatttc tcttgccata gctcttacaa taccactatt tctagcaact ccaccaacca
    10021 taactacatt tctttgtact cctattcttt ttactaggct agaaactctc tttgctactg
    10081 aagtatgaat acctgccact atatcttcaa tttttgcatt ttcagataaa tgtgatataa
    10141 cctcagactc tgcaaatact gtacatgtac tgcttattga tacttcattt tgagaattca
    10201 tagatatact tccgagttca gatacatcaa cctctattat ttttgccatt acatctaaaa
    10261 atcttcctgt acctgcagca catttgtcat tcataagaaa gtttaatagt cttccattat
    10321 tatctaattt taataccttt gcatcttgac cacctatgtc aataatggtt cttgtctctg
    10381 gaattatgaa atttacccct ctagcatgac agcttaattc acttatttgc ttatcagcat
    10441 ctgaatagtt cattcttcca tatcctgtaa ctacaacttt tttaatatct tctcttgcaa
    10501 gacctgtctt gccatataat ttttctaaaa ctcttgatgg tccagtagtc ccagtaccag
    10561 aggatattgt ttcagaagct acaatatctt ccccattctt taagattact ccctttgatg
    10621 cagttgaacc tatatctaat cccattgtgt acatctttag cattctcctt ttaaaaaatc
    10681 tagaattact aattaatatc ttacaacttt actatcttta aagttattta ttttctcttc
    10741 atcatagcct aaagatttta atacttctac agtatgttga cctacttttg gtgctggtgt
    10801 atattcttta attccttcat ttctaaacat aactggagta ttaactaaga cacctgtatt
    10861 tccgctatcg tatgttttct taaataagaa gtcatttgcc caagcttgtt cgtcatctaa
    10921 taaatcttca cagctttgaa ttttttcaaa tggtaagtct gcttcttcta ataaagctga
    10981 ccactcgtct aatgtttttt ctaacatagc ttctccaact atcttaacta aatcttcaac
    11041 atgattaacc attgaatcta tgttattata tctatcgtct tctaatatat attctctatt
    11101 tataacctta cagaatttgc ctaaccactt gttgtattgt attaaagcta attgaatcca
    11161 tcttccatct ttacatttat atgtagtcat taatgggctg tttggatttt ctcttgataa
    11221 aggcatttca tttccgtatt gtgctgttgt tatcattgtt cccattccat atatagctgt
    11281 atggaaaaga cttactgtta ctctctcacc tttaccagtt tgagcttttt tatgtaatgc
    11341 tgctaaactt cctgctgcta gtgctagacc tgcatagtgg tcaccaaatc ctgctgctgt
    11401 atttgctgga gatgttcctt tttccataac agattggcta actcctcctc ttgcgaagta
    11461 tgcagtatag tcaaatcctg gtttatcttt taaaggacct ttttcaccat atcctaatat
    11521 ttgagagaat attaatcctg gatacttatc ttttatttgg tcataagcta tacccatttt
    11581 ttctaatgct tgaactctaa cattagttac aaatatgtct gcttctgata ataatttatg
    11641 taatatttct actccttctt ttgattttac attaatactt acaccctttt tatttccatt
    11701 ttctaattca aacatagggt tttcatcatc tgatgctgga gatttaaatg ttccacccat
    11761 aactcttatt ccatcacctt ctataggttc aatcttaata acctctgcac cccagtcacc
    11821 taacattttt gcacaacatg gtgctgcaat gaaacttgaa agttctacta ctttaactcc
    11881 ttctaaaagc ataacgtttt cctccttaat agtatcaatt ataaaatttc gattaaatca
    11941 ctcataaaat acttttttct gatattaact gtttgatgtc aaagttccta atcttatcta
    12001 aaataactta aaaattatta aaattatttt ttattgtttt attgtcttac acctatattg
    12061 ttaagcattt atcgtgccaa ctttatattt aatcaaaata tcgcttgatt tgaatgtttt
    12121 ttaacaatat acatttgaat attctttttc atatcgtggc atcttgcaac tatccattgc
    12181 attttgctat tctttctatg cccataaaag ttttttttat atattttttt atgaaaaagc
    12241 ttgacatttc aaaaaaagtt ttcattgcta aactcaaaaa cacatgttat cattgtaatt
    12301 aatcagcaag ttagttaact aatttcaaac ttagataatt ttttaataat atttaatcta
    12361 aaaaataaca atttctttga taaattttta gagtaattat tacattaaat aaaaaattac
    12421 taaatatcaa aataacacag ggaaaacaat ttcaaagcta aatttttaac cttaaaaatt
    12481 ttgtataaaa aatataacct tatttgatac atattatttc taaatgaaaa attaccataa
    12541 taatttaata attacaggag ggtattgata tgaaaatact agtatttgga gcacgcgatt
    12601 atgaagaacc agtaataaaa aaatggtctg aagaacataa ggatgttcaa gtggatattt
    12661 atcctgaaaa catgactgaa gaaaatgtag ttaaagctaa agggtatgat ggtatatcta
    12721 tacaacaaac taactatata gataatcctt atatttatga aactttaaaa gatgctgggg
    12781 ttaaagttat agcttcaaga actgcagggg ttgacatgat acattttgat ttagttaatg
    12841 aaaatggact tatcgttaca aacgttcctt cttattcacc taatgcaata gctgaattag
    12901 ctgttactca agctatgaac cttttaagaa agactcctct agtaaagaaa aaagtctgtg
    12961 aaggtgatta ccgttggata gctgaacttc ttggaacaga agttagatct attacagttg
    13021 gtgttatagg tacaggaaaa ataggtgcta cttctgcaaa gttattcaaa ggcctaggag
    13081 ctaatgtaat tgcatttgac caatatccaa atagtgattt aaacgatata ttaacttaca
    13141 aagattcttt agaagacctt ctaaaagaag ctgaccttat aacattacat actcctttac
    13201 ttgaaggaac aaaacatatg ataaataaag atactctagc tataatgaag gatggagctt
    13261 acatagtaaa tactggccgt ggtggtttaa ttaagacagg ggacttaata gaagcactag
    13321 agtcaggaaa aattagagct gctgcccttg atacatttga aactgaagga ttgttcttaa
    13381 ataaaaaaat gaatccagga gaattaactg acccagaaat aaataaactt ctttctatgg
    13441 aacaagttat attcactcat caccttggtt tcttcactag tacagcgatt gaaaatatag
    13501 tttattctag tttaagtagt gctgtagaag ttataaaaac aggaactgct actaatagag
    13561 taaattagta ttttataaat atattttttc attaattagc tattatctaa caaaatagcc
    13621 tttaatttcc caaatataag agagtcagct caatatatat tgagtagact ctcttatatt
    13681 attttttata ttaaacttgt acccctctta ttcatagcaa attaaattta tattaactta
    13741 tcctaactac tcctacttta tatattttac ctatttttac ttttctaatt atatacattt
    13801 gaatgggtac aatctgtgtt ttacaaaaaa aacacctaac aaactatgaa aattgtatga
    13861 ttaatattaa tatatttata agaaaacata acaattatgt tagctctgga tttttaggga
    13921 gggagtatat tggaaaaaat gaataaagtt acttctaaaa aaactactta tattgttact
    13981 tcagcattat ttgcatccat aatatgcctg acaatagcat atatattaca tattccagtt
    14041 ggtggaaaca atggatatgt tcatatagga gatgctttta tttatctagc tgcaactatt
    14101 ttgccaacaa actatgctat agctgcttct gcaattggtg ctggtctagc tgatttatct
    14161 acaggtgctg ctatctgggt aataccaact ataatcataa aaccaatact tgtattattt
    14221 ttcacttcta aaagtgacaa gataataaat aagagaaata tagttgcttc ggttgtagca
    14281 ggtatagtag gattagtttt atacatgttt gctgaaggta taattattgg aagttttaca
    14341 agtgcatttg taatgagctt acttggctta ttacagccaa taggaagttt tattgttttt
    14401 ataatcttag gtatggcact tgataaactt gattttaaaa aacgttattt taattaagaa
    14461 tttaaattta tggagggaaa attatgaata tcttatatac aattgaaaat agtatctatg
    14521 taaatataac taatacatgt ccatgtagtt gtgttttttg tataagaaac gaaaaagatg
    14581 aagttgcaaa tagtggtagt ttatggttgg agcatgaacc aagtgtagat gaagtaaaag
    14641 aagccttcaa taaatacaac ttagacgcct atgatgaaat cgtattttgt ggatatggcg
    14701 aaccattaat gagaatagat gaactgatag aggtagcaaa gtttataaaa gaaaaaagta
    14761 gcataaagat aagaataaat acaaatggtc taagtgattt aatacataac aaaaaaacag
    14821 ctattctgtt aaaagatgtt atagatgcag tatcaataag tctcaatgcc ccaaataaag
    14881 aagcttacaa tagagttact caaccaaagt ttggagaaaa atcatttgat tatatgttag
    14941 attttgcaag atattgcaaa aaatatataa aagaagtagc tttttcagta gtagatgaaa
    15001 tatctccaga agaaatagaa gaatctaagc aactggcaaa aaaacttgat ataccattga
    15061 gagttagaca taagaattag caagtaaaat tataaaacat taagaatcac ctgaatggtc
    15121 tttcaggtga ttcttcctct tgtattaaca ttttacaaaa tagatttcat accacactag
    15181 gaatgtgtaa atcatccaaa tctttctcca attatcagag tttccactaa tgtagtcatt
    15241 aaaaatctca ttaatctcat ctatgttaaa gaatttatct gctatctcac tattgaattt
    15301 atctcggata tccttattat attggtcatc agccatccat atacgaattg gcacaataaa
    15361 tcccaattta tttctaaaag caatctcttc tggtaatgtt tttgatgcag ctgtacgaaa
    15421 ggcaatttta ttttcttttc ctttcacttt atattccgat ggtattctgg atgcaatatc
    15481 aaatattctt gtatctgtaa gaggcattct gacttccaat ccaactgcag tactcatttt
    15541 atctatatta aaataaatat ctccttccaa ccatatttga atatctacat ttgacatgtt
    15601 agtaagtgta tctagttctt ctgtttcttc ataaattctt tttaccactt taataggaag
    15661 cacatcagga ttgtatttct taagaatctt tcttttctca ccctcattca taatgttcgt
    15721 atttccaata taaaatttct tcaaattctc tccatagcac tctgccttat ggtatatatt
    15781 atatccacca aagaactcat ctgctccttc tccagagtaa cagatactcg tgctctcaac
    15841 tgctgccttg catccaatcg caaaaacgat agcagatgca tctccagttg gttgctccat
    15901 atggtacatt acataaggta ctgacgcaaa atactcttca ggtgtaatta tgtaccttga
    15961 atttttacac ccaagaatat cagctgtctg ttgtgctaat tttgattcat caaaccactc
    16021 ttcttcataa ccacatgaac ctgttttttc tatatctgac attgccaata tataagatga
    16081 atcaacacct cctgatagga ataactctgc agtttcattc tctgatttta cctctggcat
    16141 aattttctga agtgttgtat gaatttcatc tgtccattcc tcaatggact tgctgtagtc
    16201 aggatgaaat tcaggtttcc aataacgccc aataaatact gttttattct gatagacaag
    16261 gtaatgtcct ggcataagct tctttactcc acggaagaat gtattttcac ctgctacata
    16321 tgtaaaagac atataaatct gtaacatgtc ttcatttaac tctttcacaa atcctggttg
    16381 ctccataatc ttacgaatag atgaaccata gagaagcttc ccattttcag tcactggtct
    16441 cttaggcaga aaagtttctt ttccacagta tcccataatg caaaggcaaa cattccatgc
    16501 atgtgatttg ccatatcata tccccaggtc ttgtatgctt ctactaaaat tgctcgttca
    16561 cgctcatctc tacataatga agaatcgatt ccaagctcct cacagagaac tttccagttt
    16621 ctaatatgtc cattgtattc taggattttt atcatacatg tgtcttttcg tattgaatac
    16681 ttttcttcta aatttatcat atgtgtatcc tttcatatta aaacatttaa ctcttaaatt
    16741 tattgattgt ttttatttat atttatttac ttataatcat tgaaaattaa cttttttcaa
    16801 agtaaaccct ttt
//
LOCUS       DAEMAE010000071        16632 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEMAE010000071 DAEMAE010000000
VERSION     DAEMAE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.072"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1395
                     /locus_tag="KPH93_001833"
     CDS             <1..1395
                     /locus_tag="KPH93_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688654.1"
                     /translation="KYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLM
                     LFLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLI
                     LGMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVT
                     MHQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSL
                     ERKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTI
                     DFIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLI
                     RELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYI
                     KQSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDI
                     LSKCDIEKVKDILI"
     gene            1536..1844
                     /locus_tag="KPH93_001834"
     CDS             1536..1844
                     /locus_tag="KPH93_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1688655.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            1868..2194
                     /locus_tag="KPH93_001835"
     CDS             1868..2194
                     /locus_tag="KPH93_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688656.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            2233..3567
                     /gene="celB"
                     /locus_tag="KPH93_001836"
     CDS             2233..3567
                     /gene="celB"
                     /locus_tag="KPH93_001836"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG1688657.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGNVHEFIFNFL
                     QIPLLKLGNTLSATAIAYVFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGT
                     KLPNIITAQFQDMFATFGGAGSTLSLIIAMLLICKSKRIKSIGKLSILPGIFGINEPL
                     IFGLPIMLNPLLLIPFALVPTVNIIIAYFCMSTGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKILDKQYLKDEKSNVEEEDDDISFDDLVL"
     gene            3578..4096
                     /locus_tag="KPH93_001837"
     CDS             3578..4096
                     /locus_tag="KPH93_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG1688658.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAMANK
                     EDITLMKEEFCY"
     gene            4192..5151
                     /locus_tag="KPH93_001838"
     CDS             4192..5151
                     /locus_tag="KPH93_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG1688659.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEEYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            5215..6267
                     /locus_tag="KPH93_001839"
     CDS             5215..6267
                     /locus_tag="KPH93_001839"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG1688660.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVRGESENGFPFGEKVGD
                     ALNKALEISEKLGFKVTNLDNYIGYAEYGDSEDYVCVIGHVDVVHEGEGWKYHPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKSNYEEETFLGFIESMSENVLVTYKE
                     LNIENSGIILDIKVKYDFSYKLKDVLDEIKSSFPNSIEIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            6270..6944
                     /locus_tag="KPH93_001840"
     CDS             6270..6944
                     /locus_tag="KPH93_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG1688661.1"
                     /translation="MLEIIGMSAEDAKIIEDCGADRIELVSALTEGGLTPSFGLIEKV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLNSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEEIDRQKLKQL
                     VNIISR"
     gene            7498..8049
                     /gene="raiA"
                     /locus_tag="KPH93_001841"
     CDS             7498..8049
                     /gene="raiA"
                     /locus_tag="KPH93_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG1688662.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            8181..10856
                     /gene="secA"
                     /locus_tag="KPH93_001842"
     CDS             8181..10856
                     /gene="secA"
                     /locus_tag="KPH93_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG1688663.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            10871..11978
                     /gene="prfB"
                     /locus_tag="KPH93_001843"
     CDS             join(10871..10945,10947..11978)
                     /gene="prfB"
                     /locus_tag="KPH93_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG1688664.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            12077..14218
                     /locus_tag="KPH93_001844"
     CDS             12077..14218
                     /locus_tag="KPH93_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG1688665.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            14366..15532
                     /locus_tag="KPH93_001845"
     CDS             14366..15532
                     /locus_tag="KPH93_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1688666.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      15592..15709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15834..16439
                     /locus_tag="KPH93_001846"
     CDS             15834..16439
                     /locus_tag="KPH93_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1688667.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVVISLVTLPLYKKMGNIIKK"
BASE COUNT         6876 a         1751 c         3128 g         4877 t
ORIGIN      
        1 aaatacagat taaacataga tgaaatagag tacttccata tagtagatat aataattgta
       61 aaaaacataa tgtttactat agaggaggat atggatataa gacttacaga tatatcattc
      121 aagcaattga tgctattttt gcttataaat ataatacgaa taaggctagg aaattcttta
      181 aaagtatcaa atactaagaa agagcttaag ctaaaaatgc ttatattaga tatagaagat
      241 gaattgaaat ctaatttatc catagtatta aattatgaag aaaaattgtt tatagaatct
      301 ttaatattgg gtatgaacaa acaaataaat agtagtaata ttaagaaatc tttatatcta
      361 aatgaagaat tagcaagtta tacaaaaaaa attataaatt tagtatcaga agaatccgga
      421 ataaatttta ataatgatat attactttat gaacaattaa tatgtcattt gaatgttaca
      481 atgcaccaaa tgaaaagtaa tgtttactta gaaaatccac ttttagataa cataaaaact
      541 aaattttgtt ttttatttac agtaatatca agttcaatag aaattgaatt aaaaggaaaa
      601 caaataacag aagatgaaat tggttatctt acacttcact ttcaaacctc tctagaaaga
      661 aaatacagca aaagggaatc aaataaaaag gcaagtattg tatgtccatt tagttttgga
      721 gtaagcatgc tattaaaagt aaagatagaa aagagatttg ataacataaa aattattgaa
      781 acattaagag aggaagattt aaaaagagat aacttcaata agactataga ttttataatt
      841 gcatttcaag agtatgaaaa tatccaaaaa ccaattttta taacaacacc attatttact
      901 gatgaggatg agaataaact taaagagttc acaaacaaaa taaaagaaaa agacacttca
      961 tataaactta tgaacagatt aatgatgtct gattatttaa taagagagtt agaacatgat
     1021 gatatttatg aagctataac atatttaact aatttattaa ttgaaaaaca ttatgcagaa
     1081 aaagagtatt tacaatctat tataacaaga gaaaagtcat atccaaccaa tgttggaaaa
     1141 ggaatattat tccctcatgg agatatgaaa tatataaaac aatctgtttt atgttttgca
     1201 aggttaaaaa caccaataag aattagaaat ggaaaggata taaagattat acttttgtta
     1261 gcatataaaa aagatgatga tagaaaagta tttagagaat tatttaaaga aatatctaat
     1321 ttaacagagg atgaaaatat gctagatatt ctaagtaaat gtgacattga aaaagtaaaa
     1381 gatattctta tataaaattc atagtgaact atggataagc acaaaatttt ccatgtgatg
     1441 gaaaaagttg tgcatgtttt tttgttttta taagatatta tatgtttgta aggtataaat
     1501 aatctaaaag tagagaagca acttaggagg aaaaaatgga agaaatagtt ttaaagatta
     1561 taattcatgc tggaaatgca aaatctatgc tttatgaagc acttgactat gcaaaggaaa
     1621 acgattttaa aaaagcagat gaattaattg aaaatgcaaa tgaagaaatt ttaaaagcac
     1681 ataaagtaca aactgaactt atacaaaagg aagctggggg agataaatca gatatatcta
     1741 ttttattgat tcactctcaa gaccatttga tgacttgtat gagtgagaga aaccttataa
     1801 aagaaatgat tatgttaaga aaagaaattc aaaaattaaa ataaaaaaat taagggggat
     1861 ttaaaaaatg aaaagaaaag tatatttatt ttgtagcttt ggtatgtcaa caagtttact
     1921 tgcagataaa atgcagaagg tagcagatga acacaattta ccaatagaag tagaggcatt
     1981 tccgatagca gaaattgaca aaatagttga agaaaaaaat ccagattgta tattactagg
     2041 accacaagtt aaatacatgc taaaggaatt aaaaccaaag tttgaagctc aaggaaaact
     2101 tatagatata atcaatgaag ttgattatgg aactatgaat ggggaaaaag tattaaagct
     2161 cgctataaag ctaataaaat ccaaacaagc gtagaattat agtaaaaaat aataggaaat
     2221 gagggtagat agatgaatag attggaaaga atacttatgc cattggctga aaaaataggt
     2281 aaaaataaat tattaattgc aattagagat ggttttttaa tatcatcacc attattaata
     2341 ataggttcat tatttttatt agtagcaaat tttcctataa aaggatggaa tgaatttgta
     2401 ggtcaaattt ttggttcaga ttgggctgta aaattgcaac aaccaactgt agcaacattt
     2461 gaagtaatga ctctacttgc agttttagga attggatatt cttatgcaaa gcaaatagat
     2521 gtagacccaa tagcatcagc agcagttgca atagttgcct tttttataat aacaccattt
     2581 gtaataccat atacaccaga aggaacagaa tccgtttaca tggtaacagg tataccacta
     2641 ggttggatgg gttctaaggg aatgtttgta ggtatgataa cagcaataac ctctgtaaaa
     2701 ttatttgctg cagttgtaaa aaaaggatgg acattgaaaa tgccagatgg agttcctcca
     2761 actgtatcaa aatcatttgc agcattaata ccaagtgcaa tagttatggt agttttcttt
     2821 ttagttaaga tagcttttga agctacacca tatggaaatg tacatgaatt tatatttaat
     2881 tttttacaaa taccgttatt aaaattagga aatactttaa gcgctacagc aattgcttat
     2941 gtattcttac atctattctg gttctttgga ataaatggaa gtagtgttgt tggagctgtg
     3001 tataatccaa tattaaaaat tctatcagct gaaaacttag ctgcttttca agctggtaca
     3061 aaactaccaa acataataac tgctcaattt caagatatgt ttgcaacatt tggtggggca
     3121 ggtagtacat tatcattaat tatagctatg ttattaatat gtaaaagtaa acgtataaaa
     3181 tcaataggaa aattatcaat tttaccaggt atctttggta taaatgaacc attgatattt
     3241 ggtctaccaa taatgcttaa tccattacta ttgataccat ttgcattagt tccaacagta
     3301 aatataataa tagcttattt ctgtatgtca acaggattag taccacttac aaatggagtt
     3361 cagctaccat ggacaactcc aattatattc tctggatttc taacaacggg atggcaaggg
     3421 gcagttttac aattagtact tcttatacta ggaatattta tgtatatacc atttataaaa
     3481 atactagaca aacaatattt gaaagacgaa aaatcaaatg ttgaagaaga agatgatgat
     3541 atatcttttg atgatttagt actataggag gaagattatg aagaaaatag tgatgatatt
     3601 ggaccaaata caagctggag ctggaggaaa agagaaaagt aatatacctc ctgctggaaa
     3661 atcaagccca ttaggaccag gtgtaatgat ggagcaattt ttaaatgaat ctaaggtaat
     3721 agctacctta ttttgtgggg atgaattttt tgtaaataat caagaagaag taacgagtaa
     3781 gatgatagct atggtaaaaa aattaaaccc tgatgtagta atctgtggac caagctttaa
     3841 ttacgaaaac ttttctaaaa tgtcagcaat actgtctaaa aatataaatg ataaaacaga
     3901 tataccagct tttgcagcta tgtcagaaga aaatatagat gtgataaacg aatataaaaa
     3961 tgacatatgt atagttaaga ctccaaaaaa aggtggaatt ggtcttaatg attcgttaaa
     4021 taacatatgt aaattagcga aagcaatggc aaataaagaa gatataactt tgatgaaaga
     4081 agaattttgt tattagtaca agtattaatt attagcaagt ttgaatatta cactatttat
     4141 aataagcata cattagtaaa atctatataa tttaaggagg ggaattttaa catgtgggga
     4201 atgatagcga catggagaat ggccctagaa ggaataactg aggcatcaga acatttagaa
     4261 aaaggcatgg aagcaggtga tgctgtggaa cttgctgtaa gaagagttga agattatcca
     4321 ttctataaat cagttggata tggtggactt ccaaatgaaa attgtgaagt agaacttgac
     4381 gctgcattta tggatggtaa cacattagat attggcgcag ttgctggtat aagagattat
     4441 tctaatcctg tcagcatagc taggaagtta agttatgaga aggtaaataa ttttttagtt
     4501 ggaataggtg ctgaagagta tgctcataaa aatggatttg aaagaaaaaa tatgttgact
     4561 gatagagcga agttacatta taaaaagaga aaaaaagaga ctttagacaa aggattaagc
     4621 ccatatgctg gtcatgatac tgttggaatg atatctttag acaaaaccgg aaaaatgtgt
     4681 gcagctacat caacaagtgg attgtttatg aaaaaaagag gtagaatagg agattctgca
     4741 ctatctggtt caggattcta tgttgatagt agtataggag gagctacagc tactggtttg
     4801 ggagaagatt taatgaaggg atgtatatct tatgagatag ttagacttat gaaagaagga
     4861 aagcatccac aagaagcatg tgatattgca gtaaatgaac ttgataaaca gcttatcaaa
     4921 agaagaggaa aagctggtga tttatcatta gttgccatga ataataaagg tgaatgggga
     4981 gtagctacaa atatagctga tttcactttt tcagtagtaa ctaaagaaga atctcctact
     5041 gtatatattt caaatagagt agatggaaag actatacata aggttatatc aaatgagtgg
     5101 atggaagcat atgaggctag aataaaagca ccaatagaag taagtgagta aagtagtggt
     5161 tacataatta aaatagagct ttataaaata gagttttata ataaggagat ttttatgaat
     5221 atcataagta ataaaataga tgagttaaaa gaagatttat taagtgatat tatagatatt
     5281 gtaaaaatac ctagtgtcag aggggagtca gaaaatggct tcccttttgg cgaaaaagta
     5341 ggggatgcac ttaataaagc attagaaata tcagaaaaat tgggatttaa ggttacaaat
     5401 ttggataatt atattggtta tgcagaatat ggagatagcg aagattatgt ttgtgtaatt
     5461 ggacatgtag atgttgtaca tgaaggagaa ggttggaagt atcatcctta taagggagaa
     5521 gaaactaatg gaagaattta tggaagagga gtgctagaca ataaaggtcc tataatgtct
     5581 gctttatatg gattatatgc tattaaagaa ttaaatttaa aattagataa aagagttaga
     5641 ataatatttg gtacaaatga agaaagtgga tttgaagata taccatatta tttagagaaa
     5701 gaaaaagcac ctattatggg atttacacca gattgtaagt atcctgttgt atatggtgaa
     5761 aaaggtatgg caaagattag aataaaaagt aaaagcaatt atgaagaaga gacattctta
     5821 ggttttatag aaagcatgtc tgaaaatgtg cttgttacat ataaagagtt gaatatagaa
     5881 aatagtggta ttatattgga tataaaggta aaatacgatt ttagttataa attaaaagat
     5941 gtgttagatg aaataaaatc tagttttcca aatagtatcg agatagaggt aatttctaat
     6001 tttaatcctg tatattttga caaagagagt aatcttgtta aaaagcttca gctagcttat
     6061 gaaagggtta catcattaga tggaactcca gtaacgacta atggaggtac atatgcaaaa
     6121 gtaatgccaa atatagttcc atttggccct tcatttccag gacaaaaagg aatagctcat
     6181 aatcctgatg aatatatgga tatagaagat ataatattaa atgcaaagat atttgcaaat
     6241 gcaatatatg aattggcaaa ggagtagtta tgttagagat aataggaatg tcagcagaag
     6301 acgctaaaat tatagaagat tgtggtgcag atagaataga gttagttagt gccttaactg
     6361 aaggtggatt gacaccaagt tttggtctta tagaaaaggt tgttaacagt gtaaagatac
     6421 ctgtgaatgt aatgataaga catcatgcca aaagttttgt atatagtaaa gaagatataa
     6481 gtataatgca aaaagatata agtgtggtta aagagattgg tgctaatgga gttgtgtttg
     6541 gagtattgga taaaaacaac aatatagatg aaaaaaatct aaatgtttta ttgaaatgtt
     6601 gtgataatct cgatgttaca tttcatagag ctatagatga atcaaataca atagatagtg
     6661 ttaaaatttt gaaggattat gataaaatta cgaatatatt aacatcaggg gggaaaggaa
     6721 gtatagttga taatatacaa atgataaaga atatgatgct taattctaat cacataaaaa
     6781 ttcttttagg tggaggtctt aattttaata atatagaaaa gataaaggaa ttaactaagg
     6841 caagtaattt tcattttggt acagctatta gaataaacaa cagccctttt gaagagatag
     6901 atagacaaaa gcttaaacaa ctcgtaaata taatttcaag atagtatatg aatataaaat
     6961 acttagttta aataaagaga gtaagttaat tataatttgg gtatatgaat gttaataggt
     7021 attaagtatg aaattattgt tgagtatgct ttataacttt taagtagttt taaatataaa
     7081 taatgaaaga agagatagat tatggaggtt ttagatagct tatatttatt aaaaaattat
     7141 aaaggtatat tggcatataa tacatataat ttcctaaaat taccatctta tgacatgaat
     7201 tcacatatta ataaacaatt aatggagatt tttgtggata gaaatagcag taaaaatagc
     7261 tttaaataaa caaaaacaag agttattaac agttttatcc acattatcca caggtgtttg
     7321 taataatatt aatccacata agtatcaatg taactaaaaa tgataaataa ttctatcaat
     7381 tgaatagaaa aaatcataaa agtaaataat aaaaaaagga ttatgagagt tcgtgtagaa
     7441 tatatttatt aaagtataaa ttttcttata ctggcataat tgaagggaga tgtacttatg
     7501 aacataatta tatctggaaa acaaatcgaa ttaactgatg caataaaaaa taaaatcgaa
     7561 actaaactaa gtaagttaga taattacatt catccagaca ctgatgtaaa agttacagta
     7621 agtgcgagaa aagcaagaca aaaaatagaa gtaacaatag ctactgtaaa tggtcctata
     7681 attagagcag aagatatgca ggaaaattta tacacagcta tagatgttgt atatgataaa
     7741 ttaagtaaac aattaaaaaa atataaaaag agattacaag ataaacataa agacaacaag
     7801 agtataagat tccaagatgc agagataaat ctagctgatg aacaagagga ttatggggat
     7861 gaaaatcaag aattagtaat tcaaagacgt aagaaattta gtgttaagcc aatgagtgaa
     7921 gaagaagctg tattacagat ggaattaata ggacatgatt tttatatgtt caagaacata
     7981 gattcagatg aaatagcagt agtttataag cgtaataatg gtggatatgg tataatagaa
     8041 catgaataaa gcaatttaga tttaatcata ttagctatcg ataaataaat tatcgatagc
     8101 tttttaaatt aatattaaaa gtatagtttt tatggtaaaa tgaataatag tacaataaag
     8161 aacgaggaga gatgtaaaat atgagcttta tggataattt attcaacatg gcagataaaa
     8221 aagaattaaa aaagttcaat aaaactgttg atataataga ttcattagaa cctaaatttg
     8281 aatctatggc agactcggaa cttaaaaata tgacaaatat atttaaagaa agattagcaa
     8341 atggtgaaag tatagatgat atacttccag aagcttttgc agtagttaga gaagtttcta
     8401 agagagtgtt aggacttaga cactacagag ttcaaatgat tggaggtata gtactacatc
     8461 aaggaagaat agcagaaatg aaaacaggtg aaggtaaaac tttggttgca actgctcctg
     8521 tttatttgaa tgcgcttact ggaaaaggtg tacatgttgt aacagtaaat gattaccttg
     8581 caaaacgtga tagagaccaa atggcaaaaa tctatgagtt tttaggaatg agtgtaggtg
     8641 ttattataca cggtcaaaat ccaaaagtta gaaaagaaca atatgattgt gatataactt
     8701 atggtacaaa taatgaatat ggatttgatt atctaaagga caatatggta atacacaaag
     8761 agcaaagagt tcaaagagga ctaaattatg ctatagtgga tgaggttgac tctatactaa
     8821 tagatgaggc tagaacacca cttatcatat ctggacctgg agacaaatca actcacttat
     8881 attcagatgc aaacacattt gttttaacct taaaaccaga tgattatgaa ttagaagaaa
     8941 aagataaggc tgtatcttta acagcatcgg gtatacaaaa agcagaagtg tattttaatg
     9001 ttgataatat aacggatata tctcatacag aattatacca tcatataaat caagctttaa
     9061 gagcacatgt aataatgaag aaagatgttg attatgttgc taaagatgga gaaatagtaa
     9121 tagtagatga gtttacaggt agacttatgt ttggtagaag atattcagaa ggtttgcatc
     9181 aagcaataga ggctaaagaa ggtcttaaaa tacaaagaga atctaaaact ctagctactg
     9241 taacattcca aaactacttt agaatgtaca aaaaactttc tggtatgact ggtacagcaa
     9301 aaacagaaga agaagagttt aaagcaatct acaaaatgga tgttttccaa gtacctacta
     9361 ataagcttat gataagagaa gacttaccag attgtgtata caaaagtgaa ataggtaaat
     9421 ttaatgctgt agcacaagaa ataattgaaa ggcacaaagt caatcaacca atacttgtag
     9481 gtacagtatc aatagagaaa tcagaattat tgtcacaaat actaaagaaa aagggcataa
     9541 agcatgaggt attaaatgct aagcatcatg ataaagaggc agaaataata gcacaagctg
     9601 gtagacttgg agctgttaca atagctacta atatggctgg tcgtggtaca gatatagttc
     9661 taggtggaaa tccagacttt ttaactaaga gagaaatgag aagaaatgga tttaaagaag
     9721 aaatagtaaa tagagtagat actccaatag aaggtatacc tgtaaaaggt aatgaaattt
     9781 tatttgaagc tagagaagag tatgaaaaac tatttgaaaa gtttaagcaa caaacgcaag
     9841 aagaacaaaa acaagttgta gaagccggtg gacttgctat aataggtaca gaaagacacg
     9901 aatctcgaag aatagataac cagttgagag gtcgtgctgg acgtcaaggt gacccaggta
     9961 gttcaagatt ctatataggt cttgatgatg accttatgag attgtttggt agtgatagaa
    10021 tatctggtat tgtagataaa ataggtcttg aagaagatat gcctatagaa catagaatat
    10081 tatctaagtc tattgaagga gcacaaaaga aagtcgaagg taaaaacttt ggaataagaa
    10141 agcatgtact tcaatatgat gatgttatga ataaacaaag agaaataata tacgcagaga
    10201 gaaaacgtgt attagaaggt gaagatttac aagaacaaat acaatcaatg actcattcta
    10261 taatagaaga agcagttact ctttatacac aagataaagg atttgatgaa gaaggattca
    10321 aagaacatat gtataatctg ttcttgccaa aaggtagcat agaaatacca gaaatagaaa
    10381 aacttaatcc agttgaaata acagaaaaag tttatgaaat agctatgaag atatatactt
    10441 caaaagaaga acaagttggc tatgagagaa tgagagaagt tgagagagta atcttacttc
    10501 aagctgttga taaccattgg atagaccata tagatgctat ggaccaatta cgccaaggta
    10561 ttggtcttcg tgctgtaggt cagcaagacc cagttattgc ttataaaatg gaaggttttg
    10621 atatgtttga tgaaatgaat aaacatatca aagaagatac tgtaagatat ttatttaata
    10681 taacaataga aactccagtg gaaagaaaag cagttgtaga tgttgaaaat ctatcttctc
    10741 catcagatgg aacacttcca acaagtaaaa ctgttaaaaa agatgaaaag gttggaagaa
    10801 atgacttatg cccttgtgga agtggtaaaa aatataaaaa ctgttgtgga agataatctc
    10861 ggaggattaa atgttaaagt tacaagagta tagacatagt atggagcaga tggcttcaaa
    10921 tttagaagaa ttgagggttt ctctttgaca tagcttcatt gatgaaaaat atacaaatta
    10981 atgaagaaaa gatgaataaa caagactttt ggaatgataa tgaagtcgct caaagagtac
    11041 ttcaagaaaa taagtcttta aaagagacat tagaagaata tgaaagctta aaaagcttat
    11101 tagaagatat agaagtatta attgaaatag gtttagaaga agatgatgat tctgtagaaa
    11161 gagatataga aaaatccata gaatcaatgg aagaaaaatt atctgaaatg aaaataaaga
    11221 ctcttctaaa tggagagtat gataaaaata atgctatatt atctataaat gcgggtactg
    11281 gtggcttaga tgctcaagat tgggctcaaa tgcttttgag aatgtatata agatggtcag
    11341 aaagtaaggg ttataaagtt aagctcttag atataatatc agaccctgaa gcagggataa
    11401 agacagctac aattcttgta gaaggaacaa atgcttatgg gtatttaaag agtgaaaaag
    11461 gagttcatag attagtgaga atatctccat ttgacccatc aggaaaaaga catacttcat
    11521 ttgcatctat agatgtaaca ccagagttag atgaaaatat agaagtagaa attaatccat
    11581 cagatttaaa gatagacaca tatagagctt ctggagctgg agggcaacat gtaaatacaa
    11641 ctgattcagc agttagaata acacatatcc ctactggtgt agtagttcag tgccaaaatg
    11701 aaagatctca gcatctaaat aaggacagag ctatgagact tttaatggca aaattgatag
    11761 aattaaaaga gttagaacaa aaggaaaaaa tagaagatat acaaggcaaa tactcccaaa
    11821 taacttgggg tagtcaaatt aggtcatatg tttttcaacc atataaattg gttaaagacc
    11881 atagaacaaa tgctgagttt ggaaatgtag atagtgtaat gaatgggaat atagacttgt
    11941 ttattaatga atacttaaaa atgaataaaa tagtttaata aatatgcact tacagcactt
    12001 aggctgagga cgataaccct cagccttcgt tctgttttgt tatacttatg aaaaactaac
    12061 attaggagtg aataaattgg atataaatca aattttaaaa aaagagttta atcttagaga
    12121 tgaacaaata aataatacat taaaacttat tgatgaagga aatacaatac catttatagc
    12181 tagatataga aaagaaatga ctggcgaaat gagtgacgtt acactaagag aattttatga
    12241 aaagttaatg tatttgagaa atcttcaaag tagaaaagat gatgtagtta gactaataga
    12301 tgaacaaggt aaattaacag atgaaataac acaaaatata gaaaaagcaa aaacattaca
    12361 agaagtagaa gatatatatg ctccatataa acaaaagaaa agaactagag caacaatagc
    12421 aaaagaaaaa ggattagaaa atttagcatt gtcaatacta gagaataatt tagataatat
    12481 agaaatagag gcaaagaatt atcttgatga agagaaagaa gttctttcta ttgaagatgc
    12541 tctaaaaggt gctagagata taattgcaga actagtctca gatgatgcta agattagaaa
    12601 gtatataaga gaattagctt taagagaagg tatgatagtt agtaaaagtg ccactgatga
    12661 aaaatcggtt tatgacatgt attatgatta tagtgaagca gtaaaaagta tggctccaca
    12721 tagagttttg gcaataaaca gaggagaaaa agaaaacttc ctaaaggtta agttagaaat
    12781 aaataatgat aaagttttaa attacataat aaacgaatat gtaaatgata aaaatttcaa
    12841 aaataaagaa gaaatcgtaa gttcaataga agattcttat aaaagattaa ttttcccatc
    12901 aatagaaaga gaaataagaa atcatttaac agaaatagct caagaaagag ctataagtgt
    12961 atttggaaag aatgttaaaa gtttattgct tcaaccacca gttaaagaca aagtagttat
    13021 gggatttgac cctgcattta gaactggatg taaaatagct gtagtagata aaaatggtaa
    13081 actactagat tatacaacag tatatccaac agaccctcaa aatgatgtag agggagcaaa
    13141 gaaagtttta aaaggattga ttgaaaaata cgatatagat ataatttcaa ttggaaatgg
    13201 tactgcatct agagaatcag aaacttttgt atcagaaatg ataaaagaaa tagatagcga
    13261 agtgcagtat gtgatagtta gtgaagcagg agcatctgtt tattcagcct ctgaacttgc
    13321 aaatgaggaa catccagata taaatgtatc aataagagga gctatatcta tagctagaag
    13381 attacaagac ccattagcag agcttgtaaa aatagaccct aaaagtattg gtgttggtca
    13441 atatcagcat gatttaaaca aaaagagatt agaagaagta ctagatggag ttgttgaaga
    13501 ttctgtaaat agtgttggag tagatttaaa tactgcatca tactccctac tagaacatgt
    13561 tgcaggaata agtaaagcta tagcaaaaaa tataatagca tatagagaag aaaatggaga
    13621 ttttacgtca agagcacaat tgaaaaaagt aaaaagacta ggaccacaag catttactca
    13681 atgtgctgga tttatgagaa tcttagaggg taaaaatcca ctagataata caggtgtgca
    13741 tcctgaaagt tatgatatat gtaaaaaaat gattgagata attggatatt ctttagatga
    13801 tgtaaagaat aagaatatag gcgaaataga tgaaaaaata aaagaaattg gcttgagaga
    13861 attaagtgaa aaactggaag tagggcaagt tacgttaaaa gatatcataa ctgaaatcaa
    13921 aaaaccagga agagacccaa gagaagaagg tataaaacct atacttagaa ctgatgtctt
    13981 aaaaattgaa gatatacaag agggtatgac tttaaaaggt acaataagaa atgttgtaga
    14041 ttttggtgca tttgtagata ttggaataaa aaatgacgga ttagttcata aatcagaaat
    14101 gagtaatagt tttgtgaaag accctatgag tattgttaca gtaggtgata tagtagatgt
    14161 aaaagtcatt ggaattgact taaacaaaaa aagagttgcc ttatcaatga aaaaatagat
    14221 aagtttatgt atggttatag tacttaaatt ctatgtttgt aaatgaggtt attcgagtgt
    14281 ataaatgtta aaaatttgta aaagtataca gtaagaaaat atattaaaat aagaacgata
    14341 gtcttgaaaa tcaggaggaa aatgaatgat ttttataaaa aatggtaaaa taaataccat
    14401 aacaaatggg ataattcatg gagatatact tatagatgaa ggaaaaataa tagaaattgg
    14461 agaagatttg atagctcctt tagatgtaga ggtaatagat gctagtaata aacttgtgtt
    14521 tcctggattt atagatgctc atactcattt aggtttgtgg gaagatggta tagggtttga
    14581 aggagcagat ggaaatgaag agactgaccc gataacacca caattaaatc ccatagatgg
    14641 aattaatcca atggatagaa cttttaaaga agcttttgaa ggtggaataa cttctgtttg
    14701 tacaacccct ggaagtgcaa atgttatggg tggtcaatgt atagctataa agacatgtgg
    14761 aaaaagaatt gataaaatgg taataaaaaa tccagttgca tctaaaatcg cttttggaga
    14821 aaatccaaag agctgttatg gtcaggatga taaatctcct caaactagaa tggctatagc
    14881 tgctttactt agagaaaacc taaaaaaagc agaagaatat ttagaggata ttgatatgta
    14941 tgagagtcat gatgatgaag actgtgaaaa acctgaatat gatataaaaa tggaaagttt
    15001 aataccagtt ttaagaagag aaattccttt taaggcacat gctcatagag cagatgatat
    15061 gtttacagca ataagaattg caaaagaatt taatttaaag cttacattag accattgtac
    15121 agaaggtcat cttattgtag atgaattagt ggaagaaggg tttcctgtaa tagtagggcc
    15181 atctttgtca gaaaggtcta aatttgagct tagaaatttg acatttaata cagcaggaat
    15241 actttcaaat gcaggtcttg atgtatgtat aatgacagac catccagtaa taccagttca
    15301 atatcttcca atatgtgcag gtattgcagt caaacatggt atgaaagaag aaaaagcaat
    15361 agaatcaatt acgataaatc cagctaagac tttaggaata gaagatagag taggctctat
    15421 agaagttggt aaagatgcag acctagtaat ttgggataat tctccactag agattcaaag
    15481 taatgtactt tacactataa taaatggcaa agtagtgtat gagaaaaaat aacttgacaa
    15541 ttattaaaaa attaactaat ataagagtgt aaggaaaatt aaaaaatcaa agaagatctt
    15601 cggggcaggg tgaaattccc taccggcggt atagcccgcg aactacttag gtggttgatt
    15661 tggtgagatt ccaaagccga cagtaaagtc tggatgagag aagatattta tggaagataa
    15721 ataagtttat tttagtttgt ttaaactata tccataaaaa ctcgaagata gggtcttcga
    15781 gttttttgtt tttcctaaaa aatataaaca tattgattag ggggaaaata attatgcaaa
    15841 acacagccaa aagaggcaat atgatttcaa caagaacttt agtagtaatg tcaattttat
    15901 ctgcaatatc ttatttatta atgtttattc aagcaccaat accaggtata tttccagatt
    15961 tcttaaaaat cgatttatca gatattccag caatattcgg aggaatgtct ctaggaccat
    16021 tagttggatt tggaatagtt gtagtaaaaa atgtgttaca agcaataaca gcaacaacaa
    16081 ctggaggaat aggagaattt gcaaatatat taattggtgg ttcttatgta atgatacttt
    16141 gttattcata taagagaagt ggagatttaa aaggtgtact gattggtttt gtattaggaa
    16201 caatatctat gactataatt ggttcattag taaattattt tgtaatgttg ccactttatg
    16261 gtcaacttat gggactagat gctataatag gtttaggttc agctataaat cctcaagttc
    16321 atgatttatt cacttttgta atttggatga tagctccatt taatgtgctg aaggctgttg
    16381 taatatcttt agtaacattg ccactttata aaaaaatggg aaatataata aagaaataat
    16441 tagacaagaa aatataaaat taaatgtatt gaatagccat aatttattta taacttcagt
    16501 catatattgt gattgatata ttaaatatat tatggcttta ttaatttatc aaaaaatgta
    16561 tataaaataa gaaatattta gacaaattta aaatttaaaa taaaggaaga attaaaacga
    16621 ttaactatta aa
//
LOCUS       DAEMAE010000072        16615 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEMAE010000072 DAEMAE010000000
VERSION     DAEMAE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.073"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..1482
                     /locus_tag="KPH93_001847"
     CDS             109..1482
                     /locus_tag="KPH93_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688668.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILSNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KTSNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1751..2818
                     /locus_tag="KPH93_001848"
     CDS             1751..2818
                     /locus_tag="KPH93_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG1688669.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIMFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2939..3247
                     /locus_tag="KPH93_001849"
     CDS             2939..3247
                     /locus_tag="KPH93_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG1688670.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGNLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3240..4670
                     /locus_tag="KPH93_001850"
     CDS             3240..4670
                     /locus_tag="KPH93_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG1688671.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPIIELKNLTTLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIRFREKYKKNPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNSL"
     gene            4748..5122
                     /locus_tag="KPH93_001851"
     CDS             4748..5122
                     /locus_tag="KPH93_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG1688672.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVNGEYWDEVTCLFREGVESC"
     gene            5116..7320
                     /locus_tag="KPH93_001852"
     CDS             5116..7320
                     /locus_tag="KPH93_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG1688673.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEIFEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYNENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESHEYRNGTKIKPEME
                     WCADGIVLITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMNNK"
     gene            7320..8729
                     /locus_tag="KPH93_001853"
     CDS             7320..8729
                     /locus_tag="KPH93_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG1688674.1"
                     /translation="MKNSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQVTSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNIENQEEVRLIFEDTNYKLYITENVYPKIDLLNIEPTRKIIQGVF
                     EEHITTAPGMKYIKEMVNENITPTPGSVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIDNY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMFKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8714..9778
                     /locus_tag="KPH93_001854"
     CDS             8714..9778
                     /locus_tag="KPH93_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG1688675.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCKSKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKKMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9808..11229
                     /gene="nhaC"
                     /locus_tag="KPH93_001855"
     CDS             9808..11229
                     /gene="nhaC"
                     /locus_tag="KPH93_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG1688676.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11644..12108
                     /locus_tag="KPH93_001856"
     CDS             11644..12108
                     /locus_tag="KPH93_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1688677.1"
                     /translation="MTRISNITIAEQSEYCILAIRKTIDFIAEFSEFSKRSLEKISKY
                     LEERGVLSSGAPIVCFHNMDLAKLDVEVGFPVANYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPIV"
     gene            12406..12768
                     /locus_tag="KPH93_001857"
     CDS             12406..12768
                     /locus_tag="KPH93_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1688678.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYQEVGLELA
                     QIGNVLLLSGSDEALKPFIDTKSTFMVDSIDEWRNYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQE"
     gene            13364..14224
                     /locus_tag="KPH93_001858"
     CDS             13364..14224
                     /locus_tag="KPH93_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688679.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKRDRLFPKNVLKLEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14482..16596
                     /locus_tag="KPH93_001859"
     CDS             14482..16596
                     /locus_tag="KPH93_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1688680.1"
                     /translation="MSENKQVANCDIIASNSKYSMYEHDTELITFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDTKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VREKINKIKDSPYFARIDFRLEDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     VERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPRVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMRQLYNDGELVMLNKSYRSTFEIINFTKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKTEIKEFEKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGISITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
BASE COUNT         6577 a         1840 c         3130 g         5068 t
ORIGIN      
        1 aatatatgat aagataaata caatagcaac attttattaa ttttttgggt ttttatatat
       61 ttaggtactg gggtgatata gttgaaccaa gagatatttt ttaaaataat gaatgaaata
      121 ttggaaaata ttgatgaagg ggtacatttt gttaataaag acgggaaaac tataatctac
      181 aataattctg tacaaaaaat ggaaaaaatg aacagacatg atattttaag caattcattt
      241 tttgatatag tcaacaaaat gaaaataaac aggagtacac tattagaagt tttggaaaaa
      301 agggaagtta tcaaagacaa tattcaaaaa tatctggaca aaaatggaaa agagataaca
      361 gcaattaata ctacaatacc aattgatatt aaaggcgagt ttgtaggggc acttgaaata
      421 tcaaagaata tgacaacgat agaaaattta ctaaacagaa attataaaaa aaatttagta
      481 aataaagtta gtagtagaca aaaaaagggt tatgaatttg atgatatttt aggggaaagt
      541 aatttgatta aagaagcaat agcaaaatct aagaaagctt gcaagtctga tgcttcagta
      601 tttatatatg gtgaaactgg aacgggtaaa gagttattta gccagtccat tcactatgga
      661 agtaatagga gaaactatcc atttatagca caaaattgtg cagctttgcc ggaatcacta
      721 ttcgaaggta tcctttttgg aactagcaag ggaggattta caggagcaat agataggcca
      781 ggcttatttg aacaagctaa caaaggaact ttgttattag atgagataaa ttccatgcct
      841 attatgttac aggctaaact attaagggtt ttacaagaag gttatgtaag aagggttgga
      901 ggaactaagg atatacctat agatgtaaga attattgcaa cgacaaatga aagtccaaag
      961 gtaattttaa acgagcataa aatgcgaaaa gatttatatt ataggcttaa cataattcat
     1021 ataaaaattc ctcctcttag agatagaaaa gaggacatac ctctattgtg taaaaaattt
     1081 atttcaaagt ataataaaaa attgaataaa aaagtggtag gaatttcaga tgaagctatt
     1141 aaaattttaa aaaattatga ttggcaaggt aacataagag agctagaaaa cataatttat
     1201 tgtactatga gtatgatgga agatgaggta caaatagaaa caagtatgat taatttaaat
     1261 gactattata accttaaaga gaataagacg agtaataatt attctttaaa cgatttagag
     1321 gacaaaacac ttagtaagat tatcagtgag atagaagaaa agtatatata tgaatcacta
     1381 gaaaaggcat cgtacaacat tactaaagct gcatcaattt taggtatgaa taggcaaaat
     1441 ttacagtata aaataaaaaa atataatatc aaaatcttat aaataatcaa gataaaaaat
     1501 taaaatagaa gcaaaataga agcaaaacca aagtaaagta aaataaagga gcaaaattat
     1561 tataaaagag caaaatattt tgctcttttt ttatttctca taagaaatat tattttttag
     1621 ttatgaaaat acataataaa ataattatag tttcaacatt ctaataaata tttacttaaa
     1681 tttaaaagtt ggcatgattt atgctttaac taattattga gaatatataa atttttagga
     1741 gggtgtatat atgagaaaag taagagtagg aatatgggga tttggagcta tgggaatagg
     1801 tatggctaat atgattctaa aaaaagaagg tatagaaata gtatctgttt gtagtagaag
     1861 tacttcaggg aaaagtatgt atgatgtctt aggaattgaa agaggagaaa gaccagaagt
     1921 tattatcaat aaaaactatg aagaagtttt tagagaaaaa agtgtagatg ttgttttatt
     1981 agcaactgat tcatttacta aaaaggcttt tgataaaata atgttcctat taaatagaaa
     2041 aataaatgtt atatcaacag cagaacaaat ggcatatcca caagctgatg atgcagactt
     2101 agctaaaaaa atggatgaag ttgcaaagga aaatggagta agtatacttg gaacaggaat
     2161 aaatccagga tttgtattag atttactagt actggcactt tcaggtactt gtgaagaagt
     2221 tactagtata aaagctaaga gagtaaatga tttatctcca tttggaaagt ctgttatggt
     2281 ggaacaaggt gttggagtaa caagggaaga atttataaaa ggtgtagaag ataagactat
     2341 agcaggtcat gtaggttttg tagaatcaat aaatatgata gctgatggat taggttggaa
     2401 actagataaa atagaacaaa caaaagagcc aataatgact acagtagata gaaaatctaa
     2461 gtacggagag gcattggctg gaaatgttgc tggttgtaga caatgtggat atggatatgt
     2521 aaatggagaa gttttaattg aaatggaaca tccacaacag ataataccag aagcagaagg
     2581 tattaaaaca ggagactatg tatcaataaa aggtatacca aacattgatt tacaaataaa
     2641 tcctgaaata cctggaggag taggtactta tgcaatgata gtaaactcta ttccattaat
     2701 aataaatgct agaccaggtc ttaaaactat gttggatata ccagtaccta gagcaataat
     2761 gggagatata agaaatcaga tagaagttga attggaagaa gaatcaaagg ctaattagta
     2821 agataatagc tagatgatta gtaagataac agctaactaa ttggtaaaat aatagttaat
     2881 ttaaaataac agtaaattaa ttagtaagac aatagtcaat taataagggg atgaaaaaat
     2941 ggatgctaaa attaatgatt gggttattat acacaatata gtattgactc cagaagaaag
     3001 agctccacaa gttccagagg atactaagaa agtatcctta gaaatgtggg ttaaaggttt
     3061 tatacaaagt gatgcttcta taggcaactt agtagaagtt aaaacaatta caggtagact
     3121 tgtaaaaggt aatttactta aagtaaatcc atattatact catgattatg gtaaatgtat
     3181 accagagtta ctccaaatag gaatccaagc taaagaaata ttatttggag gtgtatataa
     3241 tgaataattc aacatcaatg aaagatatga gttatcaagc tgttatggga agaaataacg
     3301 aaataatgaa aaatgcaatc ggattggatt actcttcttt tgaacaagag ggaatagggt
     3361 ttgattatga gaaaatgatg agtgaaacag gatatacatt acaagatatt gaagcaattc
     3421 aatcacagta tgctgttgga aatactccta taatagagct taaaaatttg actacattag
     3481 ctagaaaatg tgctaaagaa ggtaaaggag ctagaatatt tgtaaaagat gaagctatga
     3541 atgcatctgg tagttttaaa gcaagaagag cagctactgc agtatatcat gccaaacaaa
     3601 tgggatataa aggtgtaata gcagctacca gtggtaacta tggtgcagct gtagcatcac
     3661 aagcggcaat gcagggatta aaatgtataa tagttcaaga atgttatgat tctaatggtg
     3721 taggtcaacc tgaaattata gaaaaagcga gaaaatgtga ggcatatgga gcagaagttg
     3781 ttcagttaac tgttggacca gagttatttt atacgttctt agttttatta gaagaaacag
     3841 gctattttaa cgcatcatta tattcaccat ttggaattgc aggtgtagaa actttaggtt
     3901 atgagttagc tatccgattt agagaaaagt ataaaaaaaa ccctgatata gttgtttgta
     3961 caaatgcagg tggtggtaat cttactggaa ctgcaagagg attgataaaa gctggttcta
     4021 ttaatactaa agtagtagga gcaagtgtag atttaaaagg attacacatg gctagcgata
     4081 atcaatttaa taaaaaatca tttacaacag gacatacagg ttttggtata ccatactgta
     4141 catggcctga taggtcagat gtacctaggt ctgcagctag accacttaga tatatggata
     4201 gatatgtgtt agtaaaacaa ggagaagttt tttatacaac tgaattattg gcacagctag
     4261 aaggtataga aagaggtcca gcaggaaata catctttagc agcagcattc tctttagctc
     4321 aagagttaga tgaggataaa gttatagtgg tacaagaaac agaatataca ggcgcaggta
     4381 aacatatatg tccacagctt acttttgcaa gagaaaatgg aatagatata aaatttggaa
     4441 atccaagaga agaaattgca ggagtaaact taatattacc agaaagacca gaattattga
     4501 aatgtgttga tattgatatg aataaaataa gaaagtcatt tataaaaaat tgtatatcta
     4561 ataatcacat tgataatatt gataagctaa gtaatcgaga tatagaattt ttaatggaag
     4621 aagtaaaaag ttcaagagat tttgttgttg atgtattaaa tagtttatag taattattgt
     4681 tttttaattt aatataaaat aaaaattcgg aaaattcgat taaattgata agaatggagg
     4741 agtgttcatg aaaaaaagag aggatgattt tgaagttaga agaaaacatc ttcaagaact
     4801 gagtgatgat gagttaaaag aaaggttttg gagtttagct acccagatag tagaaccaat
     4861 gatagaactt ggtaaaaaga atacaacacc atcaatagaa aggtctgtct tgttaagaat
     4921 gggattttct tctcttgaag taaaacctat tttagaaggg gttatggaaa gaggtcttat
     4981 aggtaaagga gcagggcatg tagtttataa attggctaaa tctaaaaata taacggttag
     5041 agaagcaggt ttgttattag ttaatggtga atattgggat gaagttacat gtttatttag
     5101 agaaggggta gaatcatgtt aaaggaaaat aaaaaattgg atatagacta tatattaaaa
     5161 gatttggata aatataaacc aaaaagaaga ggttgggttt ggagagaaca cttggaaaat
     5221 cttgaaatgg gaccttttaa atataaagac tgtacaaaac ctttaaagaa aagtgtaggg
     5281 ctaccatcat ctaagtattt caataatata gaccctcaac caagtccagt tattactact
     5341 gaaatagctt ctggtagatt tgaggatgat atacgaagaa tgagaatggc agctcatcat
     5401 ggagctgacc atctaatggt tatcagaact gctggacaat cacactttga tgggcttata
     5461 gaaggaactc cccaaggtat aggtggagtt ccaataacaa gaaaacaagt aagagctcaa
     5521 agaaaggctc ttgattttat agaagaagaa gttggtagac ctattaatta ccattcatat
     5581 gtaagtggtg ttgctggtcc tgaagtagca gtaatgtttg ctgaggaagg agtcaatgga
     5641 gcacatcaag acccacaata caatgttctg tataggaata taaacatggt aaggtctttt
     5701 gtagatgctt gtgaggcaaa gaaaataatg gcttttgcca atatagctca aattgacgga
     5761 gcacataatg ctaatgctac tgctagagaa gcatggaagg taatgcctga acttatggtt
     5821 caacacgctt taaactctat attctcagaa aaaataggta tagataaaag taatatatgc
     5881 ttatcaacag ttccaccaac agcacctcca gcaccatgtt taagaataga cttaccttat
     5941 gctgttgcac ttagagagtt atttagtgat tataagatga gagctcaaat gaatactaaa
     6001 tatatggaat catcaactag agaagcaaca gttactcatg tattaaactt attaacatca
     6061 gtattaacta gagctgacat acaatcaact ataacacctg atgaaggtag aaatgtacct
     6121 tggcatatat acaatataga agcatgtgat acagcaaagc aagctttagt tggtatggat
     6181 ggtcttatgg atatgataga acttaaagat gtaggtgaat taagagacaa agcaagagaa
     6241 cttaaagaaa gagctgtatt atatatggaa gaaatatttg aagttggtgg atattttgaa
     6301 tctgtagaac aaggattttt tgttgattct ggaaattatc cagaaagaaa tggtgatgga
     6361 atatctagaa aaataaaagg tggagttggt gaaggtactg tttatgaaag agaagaagat
     6421 tatttagcac ctgtaactgc tcactttgga tacaataatg tagctcaata caatgaaaat
     6481 gctatagata atccatcaat gttaatagat ggatgtacat ttgaaaatcc agacaaaata
     6541 atatatattg atgagcttga cgattttgat aatgtagaaa atagactaaa agaatcacat
     6601 gagtacagaa atggaactaa aattaagcct gaaatggaat ggtgtgcaga tggaattgta
     6661 ctgataacaa tgatgcttcc tactgataaa agaacagcag agtttgcagc tttggaattt
     6721 gtcaaaaaaa tgaacttaca agaaattgag gttataagta gagaagttat gcatgaatct
     6781 gaagggacta ggatagaagt gaaaggaaga gttccgtttg atatagattt aaacaattta
     6841 gtaataccag aagaacctaa ggtactaact gatgaagaaa taagagcaga tatagaagaa
     6901 aaacctatga aaatagtttc agctacagtt ggagaagatg aacattcagt tggtttaaga
     6961 gaaataatag atataaaaca tggaggtatt gaaaaatatg gtatagaatg ccactatcta
     7021 gggacttctg tacctgtaga aaaattggta gatgcagcaa ttgaactgaa tgcagatgca
     7081 atacttgcat ctacaataat aagtcatgat gatattcact ataaaaacat gaaaaaatta
     7141 cacgattatt gtgtagaaaa aggtataaga gataaagtaa tgattgcgtg tggaggaaca
     7201 caagtaacac ctgaaatagc cgttgaacaa ggcatagatg ctggatttgg aagaaacagc
     7261 aaaggcatac atgtagccac tttcttagtt gaaaaaagaa gagaaatgaa taataaataa
     7321 tgaaaaatag caaagtttct ttaaaaatag atgtgttggt agctgaaatt ggaagtacta
     7381 ctacagtggt aaacgctttc cacgatatta atactaacaa tcctatattt ttaggtcaag
     7441 gtcaagctcc aacaacagtg tttgaaggtg gcgatgtaag aaatggctta tcaggtgcta
     7501 ttaaagattt agctaataaa ttatctgtag atgatataga gtataatgat atgtttgcaa
     7561 cttctagtgc agctggtgga ttaaaaatga ctgttcatgg attagtctat gatatgacag
     7621 taaaagcagc taaagaagca gctttagggg ctggagctgt tattcgtcaa gtaacatcag
     7681 gtagaattaa aagaactgat ttaaataaaa taaaagaaat aaacccaaac ataattttaa
     7741 tagctggtgg agtcgattat ggagaaagag atacagctat ttataatgca gaaatgatag
     7801 cttctatgaa tctaggcata ccagttatat atgctggtaa tattgaaaat caagaggaag
     7861 taaggttaat atttgaagat actaattata agctttatat aactgaaaat gtatacccta
     7921 aaatagattt attgaatata gaacctacaa gaaaaatcat tcaaggtgta tttgaagaac
     7981 acataactac agcaccagga atgaaataca ttaaagaaat ggttaatgaa aatattactc
     8041 caactccagg atctgtaatg gaagcttcta aacttttata taaaaatatt ggagatttat
     8101 taactctgga tgtaggtgga gcaactactg atgtacattc tgttacagat ggaagtgact
     8161 atattaataa aattttagtt aatcctgaac ctactgcaaa gagaagtgtt gagggggatt
     8221 taggtgtata tgtaaatatg aagaatatag tagaagttat aggtaaagaa aacttacaaa
     8281 gtgaactatc tatagatatt gatgcagtaa tagataatta tcctccaata ccaaaatcaa
     8341 aagaagaaat attatttgtt gagaggttga caaaagaagc tgttgttaaa gctatgctaa
     8401 gacattcagg gaaaataaga aatatctatg gaacaactgg aaaagtcaaa attgcagaag
     8461 ggaaagattt aacagaagta agatatatag ttggcacagg gggagcactt actaggctcc
     8521 ctagtagaat tgaaatatta gacaatatgt tcaagtacaa taaaaataat gaattactat
     8581 ttccaaagga aaaaacaaag atattaatag ataatgacta tattatggca tctatgggtg
     8641 ttttatctaa aaaatatgaa gaagcatctc ttaagctcct gttaaaaagt ttgaattttg
     8701 aggaggaaag attatgtatc ctaggttaga aatagatata gaaaagttaa agcataatgc
     8761 aaaattaatt tcgcagatgt gtcatgaaag aaatattaag atatcttttg ttactaaatc
     8821 tttttgtgca caaaaagaaa tagtagaaga aatatgtagt gaaggaatag accatatagc
     8881 agattcaaga atacaaaact taaaaaaatt acaagatata aatctaccta agatattgat
     8941 tagaatccca atgttaagtg aattagaaga agttatcaat tattgcgata ttagttttaa
     9001 ctcagaatta ggaacaataa agaaattaaa tgaattatgt aaaagtaaaa atattattca
     9061 taagatagtt cttatgtttg acttaggcga tttacgagaa ggatattttt atgaagaaga
     9121 cttttttaat aatgtaagag agatagtaag attaaaaaat attgaaataa ttggtatagc
     9181 tacaaactta acctgttatg gagctattat accatctagg gaaaatatag gcagactggt
     9241 aagtatagca aaaaaaatgg aagaaaaatt tggaataaat ctagaaatag tatctggagg
     9301 taattcaagt tccattcatc tgttgataaa taataatatg ccagaaggta ttactaattt
     9361 gagaataggc gaatctattc tacttggtag agaaacggca tacggtgaaa atataaatgg
     9421 aacatatcaa gatgcattta aattaatatg ccaagtagtt gaatgtaaag aaaagccatc
     9481 agttcctatt ggagaaatag gagttgatgc ctttagaaat aaacctgttt atgaagataa
     9541 gggaatctta aaaagggcaa tcatagctat aggtaaacaa gatataaata ttgactcttt
     9601 aattccaata gacactgata taaaaatatt aggggcaagt tcagaccaca tgatactaga
     9661 tgtaagtaac accaaatatg attataaatt aggtgacaat ttagaatttt tattaactta
     9721 tgggggtatc atgtcatcta gtacgagcaa atatgtttca aaaaaaatta tagtgtagtg
     9781 tttaaataat taggaggaaa ctattttatg aaagagcgag aatgtagaaa agcaagtctg
     9841 tttgatgcaa tgataccaat tacttgtttg atattatttt taagtctagg agtattagtg
     9901 tatggttcat cacctcatgt accactaata ggagcagcag cagtagcagg acttgtggct
     9961 gtatatagac ttggatttaa atggaaagag ttagaattaa gtatgtttaa tagtataaag
    10021 atggcaatgc aagcaatctt aattataatt ataataggcg tacttatagg aacttggata
    10081 gtaagtggag tagttccatg tatgatttac tggggactaa aaattttatc accaaatatt
    10141 tttttagtag catcaacatt agtctgtgca atagtatctc ttgcgacagg ttcatcttgg
    10201 agtacaatgg gtacagtagg agtagctctt ttaggaattg gtcaaagttt aggtatgcca
    10261 attggattaa tcgtaggttc cataatatct ggagcttact ttggagataa actatcacct
    10321 ttatctgaaa cgactaactt agctccagca atggcaggaa cagatttgtt tacacatata
    10381 aaatatatgc tatattcgac tataccatca ttattaattt gtttagtaat ttatggtgta
    10441 attggaatga agtattcagg tcaagcacta gatataaaac aaatagaatt aattaggtct
    10501 acattagatt ctacatttaa tactttatca ccagtattat tattagctcc agcaatagta
    10561 attggattag ttgtatttaa agttcctgct ataccagggc ttatagtagg tgctgtattg
    10621 ggagtattgt ttgcagtagt ttttcaagga gaatctatga atacgatatt agatgcagca
    10681 caaaatggat atgtttcgtc aacacaaata caagaggtag atgctttatt atctaaaggt
    10741 gggattatga atatgatgtc cactgtatcc ttaacaatat gcgctttatc tcttggaggt
    10801 attttagaaa aaacaggtat gcttgaagta gtagcatctt ctttactaag attagcaaag
    10861 ggtgtatttg gaacagtatt atgtactatg gtaacttgta caattaccaa tataatagca
    10921 ggtgaacagt atctatctat agttatacca ggaagaatgt ataataagga atacaaaaag
    10981 agaggtatac atcctaagat gttgtcgaga gctttagaag atagtggaac tttgacttct
    11041 ccattagtac catggaatac atgcggggcg tatataacag caacattagg tgtatctgca
    11101 ctaacatatg gtccatacgc tttattaaat attataaatc caatagtatc tttagtatta
    11161 attgcatgta agtttaaaat agcaagaata gaagatgaac ctgaaacttg tttaaatttt
    11221 gatgtttgat aatagataac ttatgtaaga acgtaggaac tacgggggag cctgtgggga
    11281 attagtaaga catattttag tatgcgaaat tctatgaatc aggaatcttg tgactttagc
    11341 cataggaggt tcagattact aaatttaaat ttatattttg atatttgaat tgtttgtaca
    11401 aattttttat agaaaaagat ttcttttaga cttgcctaaa aattaggcaa gtcttttaag
    11461 tatgttctga ttataagagc actgtgtgac aagaatgatg agattatttt tgttatactt
    11521 tcgatataag aaatgaaagg aggctatgtt ttatattcat atgaaataat tagtacacag
    11581 tgaattttgg tgtaatttgg cataaaagga ttaaacatta aaataaagta atggaggaat
    11641 aaaatgacta gaatatctaa tataacaata gctgaacaat ctgagtattg tatcttagca
    11701 atacgaaaaa caattgactt tatagcggag ttttcagaat tttcaaagcg aagtcttgaa
    11761 aaaatttcta aatatttgga ggaaagaggg gttttatcct caggggcgcc aattgtatgt
    11821 tttcataaca tggatttagc aaaattggat gttgaagtag ggtttcctgt ggccaattat
    11881 atagatggaa aaaatgaaat tttacagaga attgttcctg cacagaaaat aataactgca
    11941 attgatttag gaccttatga attacaagac cctactttag aggacttatt ttcatgggca
    12001 aatagcaatg gatataaact tcatggcgat atatattacc agtatttaaa tgatatcaat
    12061 cgtccagcta gtgaatatct tacgaaaatg atgttaccca ttgtatagaa agttaaagat
    12121 tatcgtttgt tagttgataa ttagaagaac atttactgct actgcataaa ttttatccta
    12181 aataatatca aggtgattta atttatgtat tctaatgtaa ctgtagataa tcaatttata
    12241 actaacttag tgtaatttag atttaattta tgtattctaa tgtaatctca aatattgtta
    12301 aggtctactt aagatgctca aaaggctatg cttaatttat tgttatataa tttgtagagt
    12361 ggtataattt agaggtaata ttatatagat atataggggg aaaatatgaa gatatatgat
    12421 atattaacac gtttttatat tcaagatata gaaaaagcga taccttttta tgagaatctt
    12481 ttaaaagaaa aatgttcttt gcgtttttcc tatcaagaag ttggattgga actagcacaa
    12541 ataggaaatg ttttactatt atctggttct gatgaggctc ttaaaccatt tatagacaca
    12601 aaatctactt ttatggttga ttctattgat gagtggagaa attatctatt ggataatgga
    12661 gcagttgtag taagagataa gaaaaaagtt cctacaggat ataatatgac attgagacat
    12721 ccagatggta caattataga atatgttcaa catacaaagc aggagtagtt aaaaatagat
    12781 tttactactg agcaactaat tagaatctat tcttagacgt acaatttatt atttttgata
    12841 ttaacagatt ataagtcaaa ttataatatt tcaaaataga atagatattt atttacataa
    12901 aagagagtgt ttaaaaatga actttttagt tcatgaagca ctcttttttg tatgaaatca
    12961 aaatacatca atatataaaa taaactgatg aagagcttaa attaaaataa aaagattcat
    13021 aacaggaccc aatcaaactt taagataggg taagtttgta atcatactaa ataattgtaa
    13081 aaagctgttt ttagtcgtat aatataaata tttatcattt aattaaggaa tctaaaagga
    13141 atctaataag aagaaaataa taaataactt tttagattta ctaagtgaaa agttattgaa
    13201 aattaaagga ttagagtctt taatcagaaa gtaaaaagta atatattaaa tttatgaatt
    13261 gaaaacttaa acaacaaatt tgtttataaa atagcaagat tcattaaata tagctaagta
    13321 ataattgata taatgttgca tggataaaag gagggaagct gcaatgtctt acaaagtaat
    13381 taatgtcata actgctattg actatataga ggagcattta tcagaaaagc tggatttaga
    13441 tatagttgct aatgctgttc attactcaaa atatcacctg catagaactt tcacaacctc
    13501 tgttggtcta acaatgcacg attacataaa gcgaagaaag ttgacagaag cagcaaaatt
    13561 attggtattt tcaaagaaac caataattga aattgcattg atagctggat atgaaagtca
    13621 acaggcattc acttctatat ttaaagctat gtacaaaaaa tctccaaata aatataggaa
    13681 agaacaagaa ttttatcctt tgcagctgag atttgttttg aaaagagata gactatttcc
    13741 caaaaatgta ctaaagttgg aaaaagaaat taaacttgca agtatgtctg acataccact
    13801 atggatgaat ttagtgagat tagtcataga tggatttcca aatttgcaag aggaagaata
    13861 tatatatcaa ttagaacagt atattttaga agaaagagct ttaatattaa aacttaataa
    13921 tgtcgctatt gcaaatatgg tgtttaatag agaaactaga agtattgatt tttttggaat
    13981 acatccacag tatcgtaatt cagacattgc acaagtgttt ttgaagaaag ttattgaaga
    14041 tttcttgata gatacagata tctcaattac tacctttcga gagggtgaca aagctgatac
    14101 aggacataga gatatgataa agaagttggg gtttgcagaa gcagagttgc ttgtggaatt
    14161 tggatatcct actcaaaaat ttattcttcc ttgtgtagaa gaaagagata atagacaaaa
    14221 ttaattgtag attaaatgca aagtttacca catatttttt gtttgaatta ttttatatat
    14281 aagagtattt ctgcttttaa tgtattttgc ttgtaagtgt acttatggta ataaatatag
    14341 aaagatttag ttttttaaaa aaagaacttt aaagctaaaa agctaacatt taagttaaat
    14401 agcaagaatg attaaaaact ccaaaacaca attaagtata atgattcagt gtaagtactt
    14461 aattaacaaa ggaggattta aatgtctgaa aataaacaag tagcaaattg cgatattatt
    14521 gcatccaatt ctaaatatag tatgtatgaa catgatacag agttaataac tttttttcca
    14581 gatgagattg cacatctaat agagataaat aacaagctag acaatgcttt taagaaagct
    14641 gaaaatcttg ttgacaagct tgataaggat tatatggaca ctaaaatgta tatggtaaaa
    14701 aatcgtggtg aaatagaccc acatgaaatg tttcaaaatg aacagggatt aaaacaaata
    14761 gataactatg gggcttttat ggtaaaagtt cgagagaaga ttaataaaat taaagattct
    14821 ccatactttg ctagaataga ttttaggctg gaagatatgg atgatgaatc taagtattat
    14881 attggtagat ttgcatttga ttatgaagat gagcttataa tacttgactg gcgttctcca
    14941 attgccagta tgttttatga ttatgaaata ggaaaagcag gatatgatgc tccaatagga
    15001 tgggtggatg gagaaataac aagaaaacga caatttaaaa ttaaaaatgg aaaattggaa
    15061 tatgcactag aaagttccat taatatacaa gatgatattc ttcaaaagga acttagtcac
    15121 acttcagatg aaaagatgaa gtcaattatt tcaacaatac aaaaggaaca aaatcaaatt
    15181 attagaaatg ataaagctga tacactgatt attcaagggg tagctggttc tgggaaaaca
    15241 tctatagcat tacacagaat tgcttttctt ctttatcgat ttaaagataa aatatcagca
    15301 aacaatgtaa ttatcctatc acctaataaa gtctttggag actatatttc caatgtactt
    15361 cctgaacttg gagaagagcc attatgtgaa ttaagttttg aaaatatagc agaagtacaa
    15421 ttagatagag ttattaattt tgaaagtgag aaagacccat tagaaataaa tgatgctaaa
    15481 tgggttgaaa gagtgcgttt taaatctaca cttgattttg taaaacttat tgatgattat
    15541 ataaaacaaa tgccgaataa gatttttatc ccaaaagact acacttttgg aagttttaca
    15601 gctaaaagtg actggataca gagtagattt gaggcatata atagatatcc agttaagaag
    15661 agattagaga aggtagctga agacattcat tataaatttg aatcagataa tattatggaa
    15721 gaagatttac caagggtaaa aagtatactt aaaagtttga atggaatgtt gacaataaaa
    15781 aatactctta cactatataa ggatttcttt aaacaaatga atatatcaaa catgtttgtg
    15841 atggcagaaa agaaaacact tgaatggtca gatgtatacc catttatata tattcatgct
    15901 gcatatgaag gtatacaaga agataagatt ataagacatg ttgttattga tgaaatgcag
    15961 gattatactc ctattcaata tgctgtaata aacttgcttt ttaagtgtaa aaagactatt
    16021 cttggagact ttggacaatt ggttaatcct aatcatactc atacactaga tgatatgaga
    16081 cagctatata atgatgggga actagttatg ttgaacaaga gttaccgttc tacttttgag
    16141 attattaatt tcactaaaaa gattcaagac atatcttcat tagagcctat tgaacgacat
    16201 ggcgaagaac cagcgttatt aaaatgtaat aacgaacaag atgaaataaa taagataaaa
    16261 acagaaatta aagaatttga aaagagcgat aatgctactc ttggtattat attaaagaca
    16321 gataatgatg ctaaagctgt atatgatgcg ttaagagaag aatatagtgt tcacttaata
    16381 tcttctgaaa gttctagctt tacaaaagga atatccataa cttctattaa aatgtcaaaa
    16441 ggtttagagt ttgatgaagt tattgtacct tcagtaaata ataaaacata ttatagtgat
    16501 tatgaccgca gtttgttata tattgcttgt acacgagcta tgcacaaact taagttaaca
    16561 tatgttggta cattaactca acttataagc atgtagatta tatcttatat gcatt
//
LOCUS       DAEMAE010000073        16598 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEMAE010000073 DAEMAE010000000
VERSION     DAEMAE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.074"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1164)
                     /locus_tag="KPH93_001860"
     CDS             complement(<1..1164)
                     /locus_tag="KPH93_001860"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG1688681.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINVENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKD"
     gene            complement(1187..1852)
                     /locus_tag="KPH93_001861"
     CDS             complement(1187..1852)
                     /locus_tag="KPH93_001861"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG1688682.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKSNTILLTKNVGDFVNLESDVIGKYVNNFMVNNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1886..2998)
                     /gene="ribD"
                     /locus_tag="KPH93_001862"
     CDS             complement(1886..2998)
                     /gene="ribD"
                     /locus_tag="KPH93_001862"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG1688683.1"
                     /translation="MSFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCIIVKDSNV
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEEEARDLNEAFFYYIKNKKPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESKKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIYGDILIESDVLN"
     regulatory      complement(3130..3251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3440..5923
                     /gene="recQ"
                     /locus_tag="KPH93_001863"
     CDS             3440..5923
                     /gene="recQ"
                     /locus_tag="KPH93_001863"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG1688684.1"
                     /translation="MNKNLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFLELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDKLYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNINSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMVLANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6012..6123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6265..7566
                     /gene="thiC"
                     /locus_tag="KPH93_001864"
     CDS             6265..7566
                     /gene="thiC"
                     /locus_tag="KPH93_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG1688685.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7620..7814
                     /gene="thiS"
                     /locus_tag="KPH93_001865"
     CDS             7620..7814
                     /gene="thiS"
                     /locus_tag="KPH93_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG1688686.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7807..8610
                     /gene="thiF"
                     /locus_tag="KPH93_001866"
     CDS             7807..8610
                     /gene="thiF"
                     /locus_tag="KPH93_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG1688687.1"
                     /translation="MVKIKIFVNESLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELENLLIARHTPNIHNKLKKGKVAILGLGGLGSNVA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDIIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8672..9442
                     /locus_tag="KPH93_001867"
     CDS             8672..9442
                     /locus_tag="KPH93_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG1688688.1"
                     /translation="MDKLVLGGHEFSSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAKEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9459..10562
                     /gene="thiH"
                     /locus_tag="KPH93_001868"
     CDS             9459..10562
                     /gene="thiH"
                     /locus_tag="KPH93_001868"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG1688689.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDLDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10563..11252
                     /locus_tag="KPH93_001869"
     CDS             10563..11252
                     /locus_tag="KPH93_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG1688690.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMKSKDIK
                     KTISNYNEKLN"
     gene            11440..12834
                     /gene="yfcC"
                     /locus_tag="KPH93_001870"
     CDS             11440..12834
                     /gene="yfcC"
                     /locus_tag="KPH93_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG1688691.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13351..14718
                     /locus_tag="KPH93_001871"
     CDS             13351..14718
                     /locus_tag="KPH93_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG1688692.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILSYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKL
                     IQIFE"
     gene            14743..15351
                     /locus_tag="KPH93_001872"
     CDS             14743..15351
                     /locus_tag="KPH93_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG1688693.1"
                     /translation="MRHFSNTYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHEKLFIEIAMEAFKNFDELIIISNNEELYSKYNIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            15399..15770
                     /locus_tag="KPH93_001873"
     CDS             15399..15770
                     /locus_tag="KPH93_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG1688694.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDIILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            15949..16287
                     /locus_tag="KPH93_001874"
     CDS             15949..16287
                     /locus_tag="KPH93_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG1688695.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         6406 a         1830 c         2823 g         5539 t
ORIGIN      
        1 atctttttta gtttttaaat atagcttatt aaagtcatta atttcttcat taatagggat
       61 tctattttcg atttttattc catatttctc taactgattt attttatcag ggttattact
      121 taaaagattt atacttttta tgtttaaatt ttttaatatt tgagcagcca tatagaaatc
      181 tctcatatct tcttcaaatc caagcattaa atttgcttca acagtatcat aaccttcttc
      241 ttgaagttta taagccttaa ttttatttaa aagaccaata cctctacctt cttgcttcat
      301 ataaattaaa actccacttc cattttccac aattttcttc atagaagatt ctagctgtaa
      361 tccacaatca cattttagag agtggaacac atctcctgtt aaacattcag aatgaagtct
      421 aactagagta ttttctacat ttatatttcc ataagtaagg gcaatgtgtt cttcatttga
      481 ataagtatct ctatatccta tgatttcaaa gtttccatat tttgttggaa ggaaagcact
      541 agatactttt tctatagtag tttcatttat ttttcggtat tctataaggt cttttatagt
      601 gattattttt aaattatgct tttctttgaa aatcattaaa tcatcaactc ttgccattgt
      661 accatcatct ttcattattt cacagataag accaatctct ttaaaacctg caagtctagc
      721 aaggtctact gaagcttctg tatgtccatc cctgacaaga actccaccag tttttgctat
      781 taaaggaaag atgtgaccag gtgttttgaa atctttactt gtagaatttt catcacagaa
      841 taattttaca gtttcagctc tttcatatgc agatatacca gttagagtat ctgccccatc
      901 tatagtaaca gtaaaggctg tttgaaatgt atctgtattt tctctaacca taagaggtat
      961 attaagactt ttaaattttt tttcagtggc aggtagacaa attagacctc taccatatgt
     1021 agccataaaa tttattgatt caggagtagc catttcagct gccatcaata aatctccttc
     1081 attctctcta ctttcatcat caaccacgat taccattttt ccatttttta tatcttcaat
     1141 agcttcttca atagtattaa acatgtaatt cactccttta ttatttttaa aacccatttt
     1201 taaataaaaa atctttatct atatttgatt tatggttact actattagaa tttaattcct
     1261 tgtagttatt aaccataaaa ttattaacat actttccaat tacatcactt tctaaattaa
     1321 cgaaatctcc aacattcttt gtcaagagaa tagtgttact tttagtatga ggtataatag
     1381 aaaccttaaa tatttcatca tctaaatatg atatagttaa acttacacca tcaatagcaa
     1441 cagaaccttt gagtatcatt gatgataaaa gttttttatc aggtcgtatg cttactaaaa
     1501 ctgcattttc atctttttca aatttacaga tagttccttt accatcaaca tgacctgtta
     1561 ctatatgacc accaaatctt gaagaaagac tcatagctct ttcaagattt actttgtcat
     1621 ttgcatttaa taaacctaaa tttgtggaac gaattgtctc catcataaca tcggctgtaa
     1681 attcattttt tccgaggttt gaaacagtta gacaaactcc atttacggct atactatctc
     1741 caagatttgt gccatctaat attttatttg atagtatggt tacttttcca ctcttaccat
     1801 tagcagtaat ttttctaagt attccaactt cttctactat gccagtaaac atcctaacct
     1861 cctatatgca tgtattaaca aaaatttaat ttaaaacatc tgattctata agaatatccc
     1921 catatatcaa agttacatta tttattgtta actgtgttga atctgacaaa tgatttattc
     1981 cttctccaga aacgaatgta ggtgcatctt taccacctat aatcttgggt gatagaaata
     2041 atttgacttt atcaactaac ttatttctaa agagactgtc attgagagta gcaccacctt
     2101 caactagaac agaatcaatg tttaactctc ctaatttata cattacaaat tccaaatcta
     2161 ctaagttatt ttttgaagga gaaattattg tttcaacatt attttctttt aacatagata
     2221 attttttact ttccttgcaa catgtgaaaa ctatagtttt actggtttta tctttaacca
     2281 aattactatt aagtggcaac tttaggtgtg tatctattac tattcttgtg ggatgagaca
     2341 ctttttcttg attgagccta caagttagta aaggattatc gttcaaaact gtatttatgc
     2401 caaccattat actttggtat ttatttctgt atttatgagt tagatacctt gatgattcat
     2461 tagatatcca ttttgattct agtgattttg tagcaatttt accatctaga cttactgctg
     2521 attttacaat acaaagaggt tttttgtttt ttatataata aaaaaatgcc tcatttaaat
     2581 ctcttgcttc ctcttctaat attcccacag taacatcaat attgttatct tttaattttt
     2641 ttactccatt accacaaaca agtggatttg ggtctaatgt agatattaca actctcttaa
     2701 tcttattttg tataatttta tctacacaag gaggcgtttt tccataatga gaacatggtt
     2761 ctaagtttac ataaagagta gagtctttta aactttgttt agcactgttt atagcattta
     2821 cttcagcatg attagagcca aatttctcat gatatccttt gcctattaca ttactatctt
     2881 taacaattat acatccaact aatggatttg gatttacaaa gccttctcca tttttagcca
     2941 attcaatagc ttttttcata taataaatat ctttttcttt ttgattcaca aaactcacaa
     3001 tatgtacacc tcctgtttaa ttggataatt tttaaacaaa aaaccctgag aattaactca
     3061 gggtttagaa taatctacaa taaatgttta ataagtgtag aacaaaattg ctctacaaaa
     3121 acactcatta aatatcttct tccatccaga ctttactgtc ggctttggaa tttaaccaaa
     3181 tcaaccaagt tttaccttgg ctcgcgggct ataccgccga ttgggaattt caccctaccc
     3241 cgaagattaa gtattaaatt tatatttaga atgtatcata aattatttta ctttgcaaac
     3301 tattttttta aaaaatatat gttaatatat aatatagaat gtctataaac tttgaaatac
     3361 cacgattgtg aatataaaaa taaaaatttg aacataatca ggaaagaagt ctaattaatt
     3421 taaattagga gagtaatata tgaataaaaa tcttttagaa atactatcaa aatactatgg
     3481 atatacaagt tttagaaaag gtcaggaatc tataataaat tctatcttaa gcgagaaaga
     3541 tgtgttagct attatgccaa caggaggggg aaaatcaata tgttatcaaa ttccagcttt
     3601 gattttagat ggaatgacaa tagtaatttc tccacttata tcacttatga aagaccaagt
     3661 agatgcttta aagactatgg gaattagtgc tgcttttata aatagttcat taagtagcaa
     3721 agaatttaat gaaatattaa ataatattag aaaaaataat tataaaatac tctatattgc
     3781 tccagagaga ttagatgcac aagagtttct tgagctaata aataataaca atgtaagtca
     3841 agttgcgata gatgaagcac attgtgtatc tcaatggggg catgatttta ggttgagtta
     3901 tagaaggatt tctgatttta taaaaaattt gcctaaaaga cctatagtta cagcatttac
     3961 tgcaacagct tcagaagagg taagaactga cataataaat ttactgtgtt tagaaaatcc
     4021 agattattat ataactggat ttgatagaga gaatttatca ataaatatag taaaatcatc
     4081 tagtaaaaat aagtatattt tagattatat tcaaaatcat aaaaatgagt ctggaattat
     4141 atatgttgcg actagaaagg aagttgaaaa tatatataat ggtttatcta agagaaatat
     4201 atctgtgtca aaatatcatg caggtttatc taaaaatgaa agaagtaaaa atcaagaaga
     4261 ttttataaat gataatgtgg atataatggt agcaacaaat gcatttggaa tgggtattga
     4321 taaacctaat ataagatggg ttatacatta caatatgcct caaagtatag aaaattacta
     4381 tcaagaaata ggtagagctg gtagggatgg agaaaaaagt gagtgtgtac ttttattttc
     4441 tccaggagat gtacatatac aaaaatattt aattgatatt ggagttgaaa atcctgaaag
     4501 aaaattattt cagcataaga agcttcaata catgattgac ttagtttaca gtaattcatg
     4561 ctatagaaag acaatattga gttattttgg agaaaattat atagacaatt gcaataattg
     4621 tagcaattgt ttagtagagg gtgaaatagt agataaaact atagatgcac aaaaagtcat
     4681 ttcatgtgta gctagaatga aaagaagtta tggtgttact actattgtag atgtacttag
     4741 aggttctaag aataaaaaga ttttacagct tggattaaat actttgtcta catataacat
     4801 tatgagagat tattctagtg aagacttaaa gaattttata aatacattgg tttctcatgg
     4861 ttttttagat ttagttgaga ctctaggcaa tggaaataga ggtagttttc ctactataag
     4921 attaaatgag atgtctatga aagttttaaa aggtgaagtg aaagtagagt ttaaagaaat
     4981 tgttatgaca aaatcattgg aagtagagga taagttatat aatgctttaa gagaacttag
     5041 acattcaatt gcaagtgaag aaaggatagc tccatatatg gtatttggag atggtacact
     5101 tagagccatg tctagtagtt atccaattaa taaagaagaa atgctagata tctctggagt
     5161 aggagaaatt aaatatcaaa aatatggtag agattttgaa gcagtaatta aagaatatgt
     5221 cgaaaaaaac aatataaata aaaaaataga tgaaaaaaat gtcgatttaa agaacatcaa
     5281 tagtgatttc ttagaggtta atacagataa agttttatat gaaaaactgt tagtcattag
     5341 agaagaatat gcaacaaagg aaaaattacc tgcatatatg gttttagcca ataaggcttt
     5401 gaaagaaata agtggaagat atccacttga tgaagaacaa ttaaaagata tttctggcat
     5461 aggtggagta aaatttgaaa aatatggagc tagtattttg gcagaggtta ataaatatgt
     5521 aaaagaaaat aatattaatg ttatttggga gaaaaagggc aaaaggaagt taatattaga
     5581 tggagaaagt agaaaaaata atgaaatagc attagattta ctaaatcaag gaaaagatat
     5641 acaaagtatc agtcaagaaa tagaagtttc actgtcaacc attattggat atgtgcatga
     5701 ctatgtaaag gatggacata gtatatcatt tgaattagga ctagaaaatt tttataatga
     5761 agataaaaag aagatgattt tagaagtttg cgaaaaagtt ggctatgata atttaaataa
     5821 cataaaaaga aaacttccag attcaataaa gtatgaaaat ataagagctg tgatacttga
     5881 ctcttatctt gaaaacttaa aaaataatat tatagcagag taatttttaa cttaataaaa
     5941 aataaaattt gtattattgt gttgaactaa taattttgct gtgttatact gaatttgtaa
     6001 aaataatgaa aaattagagc taggggcgct agaattggct agctgagaga taaagccgta
     6061 acttacaaac cctaataacc tgatcaagat aatgcttgcg aagggaagct ttttaatata
     6121 attttattat ataaataggc aaattattta atgttagggg aaatttgtct aagtgtatat
     6181 acgattataa gagtgctttc caaacgagga aaagcacttt ttttattttc aaaaactata
     6241 actttaatct tggaggattg taatatgaac tatacaacac aaatggatgc tgctagaaaa
     6301 ggcataataa ctaaagaaat ggaaatcgtt tctcaaaaag aacaggtaga tgtaaatgaa
     6361 ttaagagaat taatagcaaa tggacaagtt gttataccag ccaataaaaa tcataaatct
     6421 ttaagtgcag aaggtgtagg taaaaattta agaacaaaaa taaatgtaaa tctaggaatt
     6481 tcaagagatt gtaaggatat agaaaaagaa cttgaaaaag ttagagtagc aatagacatg
     6541 aaagctgaag ctataatgga tttaagtaat tatggtaaaa ctagagaatt tagagaaaag
     6601 gttgttgaaa tgtcacctgc aatgataggt tcagtaccta tgtatgatgc agtaggatat
     6661 ttggaaaaag aattaaaaga tataacagaa gaagagtttt taaatgtaat aagacaacat
     6721 gctattgatg gagtagactt tattactata catgcgggtc ttacaagaag tgtttgccaa
     6781 aaaataaaaa atcatgagag attaactcat atagtatctc gtggaggttc tttattgttt
     6841 gcatggatgg aattaaataa taaggaaaac cctatttata ctaattttga taaaatattg
     6901 gatatatgtg aagaatatga tgttacattg agtttaggag atgcttgtag accaggttgt
     6961 ataaaggatt ccactgacgg tgttcaaatt caagaacttg tagtattagg tgaacttact
     7021 aaaagagcat gggaaagaaa tgtacaagta atgatagaag gtcctggaca catggccatt
     7081 gatgaaatag aagcaaatgt agtattagaa aagagattat gccatggagc acctttttat
     7141 gttcttggac cattggttac agatatagca ccaggttatg accatataac aagtgcaata
     7201 ggaggagctt tagcatgtgc taaaggagtg gattttttat gttatgtaac tccagcagaa
     7261 catttaagat tgcctaattt agatgatatg aaagaaggta taatagctgc aaagatagct
     7321 gctcatgcag gagatatagc taaaaatgtt aaaggtgctc gtgaatggga taataagatg
     7381 agtaaagcta gagctgattt agactggtgt gagatgttta ggcttgcaat agaccctgaa
     7441 aaggctaaga gatacagaga tgaatcaact cctactcatg aagatagttg tactatgtgt
     7501 ggaaaaatgt gctcaatgag aacggttaag aaaatattaa acaatgagga acttaatcta
     7561 atataatttt tggctaatat gactggaata ataagaagct gaaaagagga ggtgatttag
     7621 tgaaggttaa tgggaaggaa attgaatttg aaaaagattt gactgttata gatttattaa
     7681 ataaatataa tttaaaaagt gatagagttg tagttgaagt gaatttagag ataattgaag
     7741 aatctaacta caatacatat gtattaaaag acgaggatat tgtagagctt attagcttta
     7801 ttggaggtgg ttaaaataaa aatatttgta aatgaatcat taacagatgt tgaggaaaat
     7861 acaactgctt ataaagttag agatagctat aacaataaat gtgatgtatt ggtgttaaat
     7921 ggatatccaa ttaaaacaga tatgcccctt tgtgaaaatg ataaattaac acttatacaa
     7981 aaaggagtta agccatcact tgatgaattg gaaaacttgt tgattgctag acatactcca
     8041 aatatacata ataaattaaa aaaagggaaa gttgctatat tgggactggg gggacttggt
     8101 tccaatgtag ctatctcttt agctagaata ggagtgggag aacttttact tatagattat
     8161 gatgttgtag aaccatctaa tttaaataga cagcagtatt ttatagatga cataggaaaa
     8221 ctaaaaactg atgcaatgat agaaaatata aaaaaaataa atccttttat aaaattaaat
     8281 aaacgagata tttttttaaa caagaacaat atggatatta ttaaggattc agatttaata
     8341 attgaggctt ttgatgatgc ttcatgtaaa gcacaggtat gtaattatgt attaataaat
     8401 ttaaaagata aatacttaat agcatcatct ggtatggcag gttattatga ttcaaatata
     8461 ataactacta agaagataaa agacaggttt tatatatgtg gggattttgt gaatgaagct
     8521 aaatttggtg aagggcttat ggctccaagg gttgctatct gcgcaaatca tatggcaaac
     8581 ttggcaacac aaatacttat agatatgtaa atattaaaat tagatattaa aaagattgaa
     8641 tattaaaaaa attaacatgg agggaaataa tatggataaa ttggtacttg gtggacatga
     8701 atttagtagc agattattgg ttggaacagg taaatatggt tcaaataata tattgcctga
     8761 ggtaataaaa gaaagtggaa gtgaaataat aacaatggca cttagaagag tggatttaga
     8821 caataaacaa gaaaacatat taacgtatat tccaaaagaa atgacaatac ttccaaatac
     8881 atcaggagct actaatgcaa aagaagctgt aagaatagct agaatatcta gaaaaatggg
     8941 atgtggagat tttataaaaa tagaagtaat atcagatact agatatctac ttcctgataa
     9001 tgaagaaact ataaaagcta caaagatact tgctgatgag gggtttatag ttcttccata
     9061 tatgacacca gacctttatg ctggaagaag acttatagaa gctaatgcag cggcagttat
     9121 gccacttgga gctccaattg gttcaaatag aggccttcaa atgaaagaaa tgataagaat
     9181 aatgatagat gagttggata tcccaataat agtagatgca ggtattggaa aaccatctca
     9241 agctatggag gctatggaaa tgggagctga tgcagtactt gtcaacactg ctatagcttc
     9301 agcaggagac cctgtacaaa tggctagagc atttaaatta gctgtagaag gtggaagaga
     9361 agcatatatc gcaaaaacag gaaatgtgtc agaatttgca aatgcatctt ctcctttaac
     9421 aggtttttta ggcaatttat agaaagtggt gattggtgat gagtttttat gatgtaatag
     9481 aaaaatatag ggattttgat tttgatggat atttaaataa tgttactgat aatgatgttt
     9541 taagaagttt atcaaaagac aagcttgagg atttggatat attaaatttg ctttctaaaa
     9601 cggcagtaaa acatttagaa gatatggcac aaaaagcaca taaattaagt gttcaatact
     9661 ttggtaaaac agtatgttta tatacaccaa tgtatatagc aaattattgt gttaatcaat
     9721 gtgtttattg tagctataat ataaagagtg gaataaaaag aaaaaaatta actatggatg
     9781 aaattagaga agagggagaa gcaatttcaa aagaaggttt caaacatcta cttgttttga
     9841 ctggagaaag tagttttcac tcaagtgtag aatatatagg tgaggctata gaaatattaa
     9901 gagagaaatt tccctctata ggtatagaag tttatccaat ggaagtagaa gaatataagt
     9961 acattgtaga taaaggtgtt gaaggattaa cagtatatca agaaacttat gacgaggaaa
    10021 tatataagag agtacatata aaaggcccaa aatcaaatta taaatttaga ctagatgctc
    10081 ctgaaagagg ggcaaaagca ggtatgagga ctttatcaat aggtgcactt ttagggctaa
    10141 atgattttag aaaagaaact ttttttacaa tattgcatgg gaaatactta aaaacgaaat
    10201 atcctcatat tgagctttca tattcaacac caagaatgag accttttaaa gggtgttttg
    10261 aggaattggt agatataagt gatactgatt tagttcaagc tatggtatgt atgagattgt
    10321 ttgaccctca tgcagctata aatatatcca ctagagaaaa tttagaaatg agaagtcata
    10381 taataccttt aggagtaact aaattgagtg caggtgtatc tactgatgta ggaggtcatt
    10441 ctcaagatga acatgatact gctcaattta agataaatga tgaaagtact gttaaagatg
    10501 tggagaaaat gctaaactca attggttatc agcatgtgtt taaagattgg gaaagatttt
    10561 aaatgtatct gataacaaac agaaaattat gtagtgagga acgatattta gaagttatta
    10621 aagagtctat attgagtggt gtagaaaata taataataag agaaaaggat ttagaatatc
    10681 aagagctaaa aaaattatat atgaaaataa aaactaaaat caattgtata gattttcaag
    10741 agcaaatatc agatgaaagt ttgaaaacta atataaatca aaaagagtgt agaaataaat
    10801 ttaaagttaa ttttataata aatagtaaca ttgaattttt tgaaaaagtg gattgtcaag
    10861 gtatccatct tccttttaaa ttattcttga acttaattga aaataagtat aattttaatg
    10921 aaaataaaat actaggatta tctttacata aagtagaaga agtagattac ttagaaaaat
    10981 taataagaaa tcaaaatata aaaatagatt acataacatt atcacatata tatgaaacta
    11041 aatgtaaaga aggattaaat ccaaaaggaa ttgagctttt aaaagaagct aaaaagatta
    11101 ctgatattaa aataattgca cttgggggaa ttttaccatc taatgtcaaa gaaacattaa
    11161 aatactgtga tgattttgct ataatgtcaa ctataatgaa gtctaaagat attaaaaaaa
    11221 ccatatcaaa ttataatgaa aagttgaatt agtaaatata gtatattgaa ttttcaactt
    11281 aaataaaaat aaaaaagtta tcttttaaat tttaaaaggt agctttttta ttttatatct
    11341 ttgctaataa aaaattgtaa aaagggaaaa ctttagtcag agagattaaa caatatccta
    11401 gaaagattta aaatttagta aagtatggag gacattataa tgactgacag taaaaagaaa
    11461 cgatttatta taccacacac ttttacaata atatttttac taatagtctt aatggcaata
    11521 ttgacttgga tagtaccaag tggtgaattt agtagagaag atgtagaagg tagaatggta
    11581 gttgtaccag ggacttatca aagtgtagaa agtaatcctc aaggatttgc agatgttttt
    11641 aaagcaccaa tagaaggctt tatagattca gctgaagttg taggttttgt tttaatagtt
    11701 ggaggtgctt ttggtattgt aaataggact ggagctatag aagcaggaat agcttcggtt
    11761 atctctaagt ttaagggaaa agaacttctt ataattccaa tatcaatgat tttatttgga
    11821 cttggaggta caacatttgg gatggcagaa gagacattac cattttatat gatatttgtt
    11881 ccactcatga catccttggg atatgattct ctaacagcaa tagcaattgt atttatagga
    11941 gcagcagcag gagcagcagc ttcaacaata aatccatttt cagttggtat tgcacaggca
    12001 ctagctcaac ttgctcctgg ttcaggagta acttacagag tagttatttt tatagcatat
    12061 atactaataa gtatagcatt tgttatgtac tatgctaaga aggtaaagga aaatcctgaa
    12121 agttcaatag tatatgaaat agataaaaat aataaatcaa tacttaatca aacttcaaca
    12181 gaaataaaag aatttacttt taaagaagta tctgttcttg ctatatttgg aatagggatg
    12241 gcaataatga tttggggtgt tttaagtcaa ggatggtaca ttccagaaat atcaatgtgt
    12301 tttgctgcaa taggtgtgct ttctggattg gttggtagat tatctcaaat tgaaatctca
    12361 gaaagtttta tagatggagc aaaagattta gcatcagcag ctcttgccat agctttggct
    12421 cgtggaattg taataattgc tcaaaatgga tttataatag atactatatt aaattccgca
    12481 gcagcctttc taggtggcct tccaaaggtt atatttgtat atttatcatt tttcttggaa
    12541 gctgcattgg catttttaat tccatcatct tcaggtcttg cttcacttac agtacctgta
    12601 cttgctcctc ttggtgattt ggttaatata tcagcacaag tgatagtaac tgcttatcaa
    12661 tttggtgtag gtcttacaaa ctttataact ccaacttcag gagtattgat gggagcactt
    12721 gcagtagccc atattccatt ttcaaaatgg gtaagattta taatgcctat cttgttgatt
    12781 ttgacagctg tatctctagt ctttttaaca ataggtattt atatagggtt ttaattaggt
    12841 gtagttttat ttaagatatt tagtaatggt ataaaatgat aatatgtttt caataaattg
    12901 ccactaattt aattagtggt aatttgtttt ttgtatttaa aaataaatat atttacttgt
    12961 gttttgaatt agtatctact tagacgttgt tttaattgtt atattttcat ttgattaagc
    13021 attgatttat tattttttat atttcatata acaatgcaaa aaataatttt catatagtaa
    13081 atgacaaaga tttacaataa atgtatattt tcttactcat tccttaaaat tactcattta
    13141 gtttaagaac tctcatttca tatgtaaagt tggcaaaaaa attaaaatta aaagtaaaaa
    13201 actaaataaa ctaatgttaa tttgaaatat atatgaaggt ttttggagaa tacttactta
    13261 tgataggaaa aaatgttaaa ataatagtgt aatcaaatat aaaggactga aataattaac
    13321 agttattaaa tattaggagt gtaaaaattt atgggattaa aagagtttga ttttattgaa
    13381 gagtctattg atatgttgag gacaatgtca ccaacattag aaactatatc agatgaaata
    13441 gaagagtatt ttgaaaatat cttggatgaa aaaaatcaag aatatataaa tgtaacctct
    13501 agaataaaat cagaatcaag tttaagagaa aagataatta gaaatagata tttaaaaaaa
    13561 tatggagaag caagtaatct tattcataat gtttctgact taataggatt aagaattgag
    13621 tgtagattta ttgaggatga aaacaaaata tatagacttt tgagaagata ttttaataaa
    13681 acagatgaca aaataaacta ttataataaa gagaataaga atattaaatt aaaactatct
    13741 gagaggcaac ccaataagca aaaaaatgga tttgaaatat ataaaataga tggagttttt
    13801 gcgtatttag atagagaagt aaaatttgaa cttcaaatta aatctcttgt aaatgtgttt
    13861 tggagtgaaa tagaacataa aataatttat aaaaacaata catatttact tgctgataaa
    13921 tttataaagg atatgatgga ctctataaag aataatctta ctatgataga taatcagctt
    13981 ttaagtattt ataaaaactt tcattcaggg aagtctttta atatgaaagt aagtaaaaaa
    14041 gaaattgaaa aattatttgc aaaactagta tatgacgctt tttctgaaaa gatgaataaa
    14101 agtataggtt ttgttgtaga ttttaaaaaa ccatgtgaga ctatattgag ctattctttt
    14161 aataaacaag atataagtga tgaagtttta ggtaatttta tgcttgatga gtttgcaaga
    14221 ttaaatgaaa ttgtaaacaa ggatatagat tttaatgagc aaatagaatt tgagagagaa
    14281 ccgacatttg atgataagtt ttgtaaagac ctaggaaatc attttagaag tagattaaac
    14341 acagaatttc catggaacct attttttaga atattatttg aaatagagcc atataacaat
    14401 accaaagact ttgaaaactt tgtagagttt ataaaagaaa atgtgctact agaagaaaat
    14461 agggaacaat tgttatgcca atttgaagaa gatagcaagc ttataattga agatatatat
    14521 gattgtatat ttaatagtat aagtgagatt gatagtgttg aaattttata taactataat
    14581 cttgaaaaaa taaactttac atcaagtgaa atcataaatt gtatatgtag agaatatgag
    14641 tgttatggtg agtacttaga agaaaaagaa aagcttatga atacatttaa agaaaagctt
    14701 attcaaatct ttgagtaata ggtcaatgct gaagtggcgc taatgcgcca tttttcaaat
    14761 acatatgaat taagtaaaat tattttggag gaggcgattt gtatagagaa aacaggtgta
    14821 attttggcag ggggaaaaaa ttctagaatg ggtagagata aagccttttt agagttacat
    14881 gagaagcttt ttattgaaat tgcaatggaa gcatttaaaa actttgatga gttgataata
    14941 atatctaaca atgaagaatt atattctaaa tacaatatta aagtttataa tgatatagta
    15001 aaagatgttg gtccaattgg aggaatatat actgctcttg aatatgcaaa gtatgatata
    15061 gttacaattg catgtgatat gccatatcta aataatcaaa tagtcgaaag aatagctaat
    15121 aaaatggatg ataaaagtgt gatttcagtg acaaatggaa agttacaacc attgtgttct
    15181 ggatataaaa agagtataat aaataaagta tctttatgta taagggaaaa tgatttaaag
    15241 ctcagaagct ttatagataa aatagataaa agttatatat actttgatga agaagattta
    15301 tttttaaatg taaacactgt tgatgagtat gaaaaattaa ctaaagttta attagctatg
    15361 gttgtaaaat agagtaggat ttatatggag gagagtttat gaatttactt aaatgcgatt
    15421 cttgggcagt aatattaaat aatagcgata aagaaagtaa agcatataaa atattagatg
    15481 aacttaaaag gaatatgtac aaagttgttg ctatagatga agagaagaaa cctattgaag
    15541 gtatagatgt gtatgaatgt ttaaaagatg ttccacacaa tattgatgtt gtagcgatta
    15601 ttgataaaca atctaagatg gatataattt tagaagaagt agagcttttg gatatacaaa
    15661 atatgtggtt tgaaaaagga agctttagtg aaatgatgat taaaaagact aaagatttaa
    15721 aattaaatat agaatataat ttaagtttat atgatgagtt gactagataa aattatacat
    15781 cttaataata aaaatataat tagttaataa aacatattat tttatagtat agattggtta
    15841 ataaaagtat agattggtta gtgaaacatt aaattacaat atttgttaaa aaaattgtga
    15901 gttacatgat ataatgtatt aaataggata ctaaggagtt gatatattat gttaatagta
    15961 actacagaaa aagtagaagg taaaaagata tcaaaggttt taggattagt gagaggaagt
    16021 acaataagag caaaacatgt tggaaaagat ataggagcaa gttttaagaa tcttgttgga
    16081 ggagaactta ctggatataa tgaaatgctc actgaagcaa gacaaattgc cataggtaga
    16141 atggttgaag atgcagaagc taaaggtgca aacgcagtaa tagcatttag actgtcttca
    16201 gcttcagtta tgcaaggagc agcagaaatg cttgcttatg gaacagcagt tgttttagaa
    16261 gatgataata gtattcttga aaaataaaat ataaatagtt aaaaagaggc atcttttaaa
    16321 aattttatgg atgccttttt ttatttgaaa aaactgattt gagaagaaat tttaattatt
    16381 atgacaataa aattagggtg atattatata aataagtaaa attatattat ataatgtatg
    16441 gtatagactt taaaaatttt atttgtagag aaaaatatgg acaatgttat aataattata
    16501 gaaattaaaa gtacttacat tcgttatgta tgtgacatgt ataattagtt ttatatttaa
    16561 tacagttttg aagtaatatc ttcattttga gaacgttg
//
LOCUS       DAEMAE010000074        16523 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEMAE010000074 DAEMAE010000000
VERSION     DAEMAE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.075"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            247..2070
                     /locus_tag="KPH93_001875"
     CDS             247..2070
                     /locus_tag="KPH93_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1688696.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHHPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIESFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMIRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICMHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPDTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHNQKI
                     NCAEALVRWLTPDKGIISPVIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVQMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEF"
     gene            complement(2398..2997)
                     /locus_tag="KPH93_001876"
     CDS             complement(2398..2997)
                     /locus_tag="KPH93_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1688697.1"
                     /translation="MAIELKKGQKINLTKKENSNLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3100..3897)
                     /locus_tag="KPH93_001877"
     CDS             complement(3100..3897)
                     /locus_tag="KPH93_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG1688698.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(4010..4115)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4207..5193)
                     /locus_tag="KPH93_001878"
     CDS             complement(4207..5193)
                     /locus_tag="KPH93_001878"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG1688699.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIKHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5676..7406
                     /locus_tag="KPH93_001879"
     CDS             5676..7406
                     /locus_tag="KPH93_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG1688700.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVAELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFIGIQIIGKR
                     VDEAEIELVE"
     gene            7516..7596
                     /locus_tag="KPH93_001880"
     tRNA            7516..7596
                     /locus_tag="KPH93_001880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7550..7552,aa:Leu,seq:tag)
     gene            7931..8446
                     /locus_tag="KPH93_001881"
     CDS             7931..8446
                     /locus_tag="KPH93_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG1688701.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKI
                     YENYSTEGMAFAVFEEFILCIIFCILIVITDNQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8922..9026
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9236..11710
                     /gene="gcvPA"
                     /locus_tag="KPH93_001882"
     CDS             9236..11710
                     /gene="gcvPA"
                     /locus_tag="KPH93_001882"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG1688702.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEMVRK
                     SAGIFDVSHMGEVQIKGVESEKFIQNLVTNDISTLNINDIIYTPMCYENGGVVDDLLI
                     YKFGEEEYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKIVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAINQTRKSKIVVSKTTHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11710..13167
                     /gene="gcvPB"
                     /locus_tag="KPH93_001883"
     CDS             11710..13167
                     /gene="gcvPB"
                     /locus_tag="KPH93_001883"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG1688703.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPIIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13590..15947
                     /locus_tag="KPH93_001884"
     CDS             13590..15947
                     /locus_tag="KPH93_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG1688704.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFENGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIISNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKITNDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(16033..16350)
                     /locus_tag="KPH93_001885"
     CDS             complement(16033..16350)
                     /locus_tag="KPH93_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG1688705.1"
                     /translation="MNEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6229 a         1922 c         2706 g         5666 t
ORIGIN      
        1 attttaataa gtggtttata attaaaaagt ttataaatta tattaatagt aataatacat
       61 aagaacttat atattagaaa aaaattttgt atatatagag aactctaaag atgaactatg
      121 taaaacaagg tttatatatt ctaattgtat agaaaatttt attggagata catgtatatt
      181 attagaaaaa atagtttaga gatattcagt aaacataaaa aataatgtag gggataaagg
      241 tattttatga agagattttt gaggagaatt atcttagttt tatttatttt attgattttt
      301 ataagtttta tttctattaa attaattcat aatgttggag attatggaaa attgattaat
      361 tatgttggga ttgttcgtgg agctagccaa cgtctaacta aactagagat gaatcatcac
      421 ccaaatgatg aattaataga atatattgat gaaatcctac aggaattgat tacagggcat
      481 ggagattatg gtttagtaat cactgattgt aatgaatata atgaggactt acttttatta
      541 gaaaaaaagt gggaagattt aaatattgaa attaaaaaag taagaatgaa agaacagaat
      601 aaccagttat taagtataag tgaagagttt ttttctcttg caaatgatac agtgtttaaa
      661 atagaaagct tttctaaaga aaaaagtaat tacttaatga ctttaataat aattatatct
      721 attataggta tattagcatg tatcatattg attttacagt atagtaaaaa aatgattaga
      781 ctggaaaaat taaatgtaga tttaaaaaat attgcatata aagatgaact aacaggagta
      841 aatactatag aaaaatttaa attggatgca aatcaaaata tttgtatgca taaggataag
      901 aaatttgctg tattctatat tgattttgaa aactttaaat acattaatga tatatttgga
      961 tatgattatg gtgacatgat actgaaaaga tatgctaatc ttatgatgaa tgatataggg
     1021 aaatatgaaa tatttgcaag agaaattgca gacagatttg ttgcgttaag atgttatgta
     1081 gataaggaag atttagtagt aaggcaacga gatgtagaca atgaactaat taatataaca
     1141 aacgaaatta aaaataaaca tagcattact gttgttagtg gaatttgttg tattgaagat
     1201 gtaaatgaaa aattaagtat agatggttta ataaatagag caaattttgc acaaaaaact
     1261 gttaagaata aaccagacac taattatgct ttttataatg acagtattcg taaaaaaatg
     1321 attgaagaaa atactatcaa gagtagaata catgaagcta tcgaaaaaag agaatttata
     1381 gtttatttac agcctaaagt taatttacat aatcaaaaaa taaattgtgc agaagcttta
     1441 gttcgttggc tgactccaga taaaggtatt atatctccag taatatttat tccagtactt
     1501 gagaagaatt tttttattgc cttagtagat aagtatgttt ttgaggaagt ttgtaaatgg
     1561 attagaaaga ggttagatga aaataaacct tttgttcaga tatcagttaa tgtttccaga
     1621 atacaatttt ataatactaa atttgtagaa acttattcca atatacaaaa taaatacagg
     1681 atacctaaaa atactataga aattgaattt acagagagtg ttgcttttga gaatcaaaaa
     1741 catcttttgg aaattataca tgatttgcat gaaaatggat ttacttgttc tttagatgat
     1801 tttggaaaag gttattcgtc tttaagtgtt ttaaaagatt taccatttga tgttttaaag
     1861 cttgactcta tgtttttcaa ggctagttta gataaagata aagagaaaat agtaataaaa
     1921 aatattgtac agatgttaaa agagcttaat atcacaactg ttgctgaggg cattgaatgt
     1981 aaggaacaag ttgaatttct tagggatatt gggtgtgatt tagttcaggg atttgtattt
     2041 tataaaccaa tgccaatatt agaattttaa gaaatattgg ataaagagtt tgtttataat
     2101 agctaaaatg tactttgtaa aatacgaact aattaagtaa aattattttg tactaaataa
     2161 atttttactt tatttacaga atattgatta tttacagaaa agcgagaaaa gtgtgttcct
     2221 ttaagtgtgg gatggatagc acaaatagga tataaaaaat attgaccata taagaatgca
     2281 taatgatata aatatttgaa aggttaccat tatatgaaaa ttaaaaagaa aataaataat
     2341 ttttcgataa aaaagagact atgagaaaag ttactcatag tctcctaaat aatgtattta
     2401 ttttcttcct gcaacccatt ttaagcccca atgaaattca gtatccattt ctctatgacc
     2461 tttgaagtat ttaactactt tttcaacact gaatgtctca tcatttacat tttctatcaa
     2521 tgctattgaa cacatattat agccattttt atgttcatca agtttgacaa ctaagtctga
     2581 atcttgtttt tgtttgattg ttacaattcc atcagcttca gaccagttag ctacaccttc
     2641 gtatataaaa gcataaataa gtattctttt tatatctttt attttattac catttattct
     2701 taaattttct ccttgtgtat tacttcctgt tctgtcatct ccatctaatt gagcaaaagg
     2761 tggattgtct aaactaccaa aggcatttcc taatgcttga acagcacctt ttacaccatt
     2821 tttcatctca aataggcaac ccaaatctaa atctatatta ctgcttctta aagagccaaa
     2881 aaaacccttt ttttgtactt tttgattcca atttaaatta actaagattt cacctaaatt
     2941 gctattttcc ttttttgtaa ggtttatttt ctgtcccttt tttaattcta tagccatagt
     3001 aatcttcctc cacaaacata gatttattta aatggtagca cttaataagt caatattcaa
     3061 caagattaga ataataatat taaaattata ctaaatatac taaaaagtag gaattacaat
     3121 acctttgtat ttttcttcaa ttaagcttct tgctttatct gatgtaagaa catcttgtag
     3181 aactttaatc ttttcactgt ctttatcttc agttctaaat gctaaaagat ttgcatactc
     3241 gtcatttata ggagctgtat aaattccgtt tttcttggca tcaagaccag caggtacagc
     3301 ataagtagtg tctataaatg cagcagtcac atctggaaga gaagatggaa tttgagcaat
     3361 atccaactct ttaaattgta ttccctttgg attttctatt atgtccttag gactaactaa
     3421 tccatccccc ttaggtaacg ttaaaagtcc aatactttca aggtatctaa ggttcttaga
     3481 acaagaagat gggttattgc ttattgcaat tgtatctcct tttttaaatt catcaacaga
     3541 ttttatctta taagagtaaa gtattgctgg gcatacatat agttttttac cagccattat
     3601 tttgtaaccc ttggccttaa cagcttcttt tagataaggt tcatgttgaa atagatttgc
     3661 atcaatctca ccattattta aagcttcgtt tggaagtatg taatcatcaa agtttttaac
     3721 ttctaaagta tatccttttt ctttaataag tggttttaat tcttctaata actctcctcc
     3781 tggaactaaa gtagcaccaa caacgatttt tttatcatct ttagctttag agcatccaac
     3841 cacagctaaa gttaatacta gacataataa caaacataat aattttttga atttcatata
     3901 aattccctcc tgaatataat tgattagtta aaaataaaat acaattcttt atcacactgc
     3961 aaaataaaaa taagcctgag tttaaactca ggctttaaaa gctaatttag actcatcttt
     4021 cagtataata ctgcaggatt tagcaccaat gtatataaaa atatactggt tgctgggttt
     4081 catagggcca gtcccttcac cactctggat aagaagtatc aatttttaat tatgttataa
     4141 atattatcat gattttttac gtctgtaaac ataattttat aaatatttta aaatctttaa
     4201 tgctttttac ttaacaccta aaattccaga aactagaata tcagtatcag cacctaagaa
     4261 atagtcattt atttcaatag aatccagagt atttctcaaa gcattttctt catgctttat
     4321 accaataagt ttattttcta caaatgcaac atcacatttt ccaaaaaaat ctccaaaaaa
     4381 ttttatacta gttattatgc ctttatcaac atttaaatta aactcaacat ttccaccaga
     4441 atattttaat tcatttgaaa gagtatattt tggtgaatta ccaaaattcc aatcccaagt
     4501 ttcgtatttt gatttagtaa gcttttctat ttgttttata tcatgttcac tcaaacaata
     4561 attagtatta tcagtatttg ttttagaaag ataatccatt atagcatctt taaattctaa
     4621 tatgtccatt ttagagtgta aatgttcact tatattggtt acacgagatt ttacagattt
     4681 tattcctttt ccttcaaatt ttataggttt aacttttaat gcttttgaga ggtcatttat
     4741 ttcagatgaa aataaaagtg taccatgatg cattactttg tttttgtagt tgtattgagc
     4801 atttccagaa aatttcttgc catctataag aaggtcattt cttccagaaa attcagcatt
     4861 gacatctagt gttttaagta aatctattat gggttgagtg aatcttctga aatcactaaa
     4921 attattattg ttacaagcta taaatgtaaa atttatattt cctaaatcat gaaatacagc
     4981 tccaccacca gactgtctcc taacgacagg tatagaatat tcttttatat attctaaatt
     5041 tatttcagaa agtgtgtttt ggttttttcc aacaattata gaaggttcat tcctccataa
     5101 aataaataag tcttcatcaa aattttttag aagatattct tccattgcta aattaaaata
     5161 agggtttgtt ttttcattat aaattaataa cattaaagta gctcctccat ataattgata
     5221 aaatttttat tatgaataca attaatttta taattcgaca ataatgtaaa aactccttta
     5281 aattagaaat tttaaaaact atcttgcaaa gtttaaataa ataatatata ctcaaaatat
     5341 gtttaataaa agtgaataga agggtagagg cgcaataatc tatcagtact aataaggagt
     5401 taagcacaga gagttattag gaaaggagat attgccgaag ttttataatc gatgctttaa
     5461 gattatattg ctgggggtac atagaaaata tgtacaactg tcacaggttt atttgtggtg
     5521 agctatcatt taaggaatta tattatattt atttttacta ttgttgatat attgtaatgt
     5581 ttaagccttg agtgtttacc actcaaggct tttttagttt tattgtaaca aataccctgt
     5641 acagttcaca aaattaaaaa gagagggaga tttatatgag gaacaaaaaa attaatatta
     5701 tttttctgac gacaatcatg tttataatgt caacagtaat ggtttttgca gaagaagata
     5761 tagacactat tgcactagct aatgcagaaa aatttgggat tttaacttta ataccaccat
     5821 tagttgctat aatacttgca tttataacta aaaacgtaat aatttcttta cttataggta
     5881 ttctttctgg tagttttata attaaagcta gtggtattaa tgtttttgcg acatttatac
     5941 aggcattttt agatttagta gatagagctt tagtatcttt agcagaccct tggaatgcag
     6001 ggataatact acaggttttg gcaattggag gagtaataaa tcttgttgct aaaatgggag
     6061 gagctaaagc tatagctgaa gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc
     6121 ttataacatg gtttttgggg cttttagtat tctttgatga ttatgccaat tcactaatag
     6181 taggtcctat gatgagacct gttgcagata aaatgaaaat atcaagggaa aaactagcct
     6241 ttataataga tgctacagca gcaccagttg ctggtttagc tataatatct acatggatag
     6301 gtcttgaggt tggtttaata catgatgctt ttgaaagtat aagtatagat gtagatgcat
     6361 ttggtatatt tttaaataca ataccattta gattttacaa tatattaata ttggcgttca
     6421 ttgtaatatc agcattatta ttaaaagaat ttggaccaat gagaaaagct gagataaaat
     6481 ctagaagcag aaaaattagt atagaccttg atgaaggcgt agcagagttg gatgatttag
     6541 ctccaaaaaa tggagtaaaa ttgagtgtat ggaatgctat aatcccaata gggacattga
     6601 taatagttgc attagcatca ttttattata gtggatatac atctataatg ggaggagatg
     6661 ataaggctct gatacaacta tttacaaatt ctccatattc ttttgaagca ataaaagaag
     6721 catttagtgc atctgatgca tctagagcat tattccagtc tgcacttgtt gcaagtttag
     6781 ttgctattat aatggctgta gtaaaaaaga tttttacaat atcggaagca attgatgttt
     6841 ggatagatgg aatgaaaagt ttagtaatca ctggtgtaat attaatactt gcatggtcat
     6901 taagttcagt aataaaagag ttaggtacag ctaaattttt aatacaccta ttatcaggtt
     6961 cactaccacc attcttatta ccaagtttaa tatttggttt aggagcaata atatcttttg
     7021 ctactggaac agcttatgga acaatgggta tacttatgcc acttgcaatt ccattagcat
     7081 actcattaaa tcctgatatg tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg
     7141 caatatttgg agaccattgt tctccaattt cagatacaac aatactttct tcaatgggag
     7201 ctggctgtaa tcatatagac catgttaaca ctcaaatgcc atatgctata tttacagcag
     7261 taatcactat tgtatttggg tatataccag ctggacttgg attgccaata tatatagtat
     7321 taccagtggc aatagctgct atatttatag gtattcaaat aattggtaag agagttgatg
     7381 aagcggaaat agaacttgta gaataaagat ataaataagt ttcacctttt attatcatta
     7441 ttttgttagt ttttagataa ttttaaaaag ttattgaaaa taatagcgaa atatagtaaa
     7501 ataaaagagt gataagcgga tgtggcggaa ttggcagacg cactagactt aggatctagc
     7561 gccattggcg tgggggttcg actcccttca tccgcacttt acagtactta attgtactga
     7621 aaaaaactgg ggttgctggc tttcggagtg ggctatcccg tttttatttt tttgtatata
     7681 tgatagattg gcactttagt ataaagtgct ttttttatag gcaaaaacag tacatttatt
     7741 tctgttttat tatcattcat ctattagaac atactttagt taagaatgtg ttaataagca
     7801 gtgttcacat tcttgataaa atgtggaata atatgtatct ttactaagac ggaaatgaag
     7861 taattaaact attaaataat attataagac gataaataag tagataaatc aaattttgta
     7921 ggaggatatt atggaaaaat atatatggtt gtttccatta ctttttattt ttcatgatat
     7981 ggaagagata ataggttttg gaatatggct taaaaaaaat aaatcaatgc ttgataagaa
     8041 gtatccattt atcagtaaaa tttatgaaaa ttatagtact gaaggcatgg catttgctgt
     8101 gtttgaagaa tttattttat gcattatttt ctgtatactt atagttataa cagataatca
     8161 gtatgtatat ttactatggt taggtagttt tatcgcatat actttacatt tggtaattca
     8221 tatagggcag tcaatcataa taagaaaata tataccttca cttattacca gtatcatatg
     8281 cctaccaata agcatttggt gtataagtaa gagtatttat atagttgatt gtgaaatgag
     8341 tacaacaatt ctgtacagta taattggtat tataattgta gcattgaatt taaagtttgc
     8401 acagtcgctg attggaaaat ttacaaagtg gatgtcaaac atataaattt cagtttattg
     8461 ggcagttcaa ggagtcagaa attggagttt tatagtctta atataatgta taaaatttta
     8521 tagcaatgta tttcataagt ataaagtgac tagaacttta agaaatatac aaattctata
     8581 gaaactaaat agtttcaagt atattttaaa aatcttattt ttcacaagtt taaaattgta
     8641 aacgatttca tcaagtctat gaatatactt ttaacatgtt atagtaaatc tcaatataaa
     8701 tttacatttg gttctttgtg tgttatttta ataataaatt aagtgaaaat gttattttac
     8761 attaatatat aatatctttg cataagagtg aataatataa aagagaaata attgataata
     8821 aaaacttaac aaaaaaatta atacataaaa aaaaataaat ataatgtgtt gacatttaat
     8881 tgaaatattc atacaataaa attgaaaata aatatttcca aatgagatta gcgagagagt
     8941 cctttatacg aaaaggcgcc gaagaagaaa tttaagaaat gccaattttt agagaagctt
     9001 tcaggcaaaa atatcgctaa ttgatgggac tctggagaga cttaagttaa gatactataa
     9061 gtcaccgaag aagcaaaata atattaaatt attaaatctt tcaggtatac aaacagggat
     9121 atataggcta ggattatttt ccatgctgat atatcttttt tttatgaaaa agtacaggaa
     9181 ataatatcaa aattacttaa atacagtagg tctaatttta tagggagggt tatatatgaa
     9241 tgaattgaaa agagtatctt tgtacaatat acacaaagag cttggagcaa aacttgttga
     9301 atttgctgga tgggaaatgc ctttagaata tgaaggtata aataaagagc atgagatggt
     9361 cagaaaaagt gcaggtatat ttgatgtatc tcatatggga gaggtacaga taaagggagt
     9421 agagtcagaa aaatttatac aaaacctagt tacaaatgat ataagcacat taaatataaa
     9481 tgacattata tatacaccta tgtgttatga aaatggtgga gtagttgatg atttattaat
     9541 atataaattt ggtgaagaag agtatttatt agtaataaat gcaggtaata tagataaaga
     9601 tgtagcatgg ataataaaac aaagtgaagg atacaatgta gatataaaaa atatatctag
     9661 tgaagtgagt caattagcaa ttcaaggacc taaagcagaa gaaattcttc aaaaaatcac
     9721 agatatagac ttaaactcaa taaaatttta taagtcaatt ccatcaacaa aagtatgtgg
     9781 ttgtccttgt ttagtttcaa gaacaggata tactggggaa gatgggtttg agatatactg
     9841 taaaaataaa tatgtagaaa taatttggaa tgaagttcta aaggttggag gagaagatat
     9901 atgtccagct ggtctaggtt gtagggatac tttgagattt gaagcagctc tacctctata
     9961 tggacatgaa ataaatgaac atatatctcc aattgaaggt ggcttatcta tctttgtgaa
    10021 aacaaataaa gaatcattta ttggcaaatc aattttatca aaagaaaaag aaagtggagc
    10081 taaaagaaag ttagtaggat ttgaaatgca aggcaaagga atgcctagaa atggatatga
    10141 tataaggatt ggtgataaaa tagttggatt tgtaacaaca ggatgtgcat ctccaacaac
    10201 aggaaaaatt ttaggtatgg gaattataga ttctgagtat gcaaaagtag gaaatgaaat
    10261 aggtatagca atcagaaaaa aagttgttcc tgctgtgata gttaagaaac ctttttataa
    10321 aaaacaatat aaaaaagata atattatatt aaacaaagaa aataaatttt catatatacc
    10381 tgctacaagt gaagataaat caaaaatgtt aaaagtagtt ggattaaatt cggttgatga
    10441 attattttca gatataccag aagaagtaaa attaaaaaga gatttaaatt tggaaattgg
    10501 aaaatctgaa ttagaggtaa gcaaaatagt aaaaagattg tcagaagaaa atttaagttt
    10561 agaagacctg acttgttttc ttggtgctgg tgcatatgac cattatatac catcaattat
    10621 aaaacatata acttcaaggt ctgaatttta tacagcatac actccatatc aagctgaaat
    10681 aagtcaggga actttacaag tagtttttga atttcaatct atgattgcag aaattacagg
    10741 tatggaaatt gcaaatgcat caatgtatga tggagcaact gctgcaatag aagcatgtat
    10801 aatggcaata aatcagacta gaaaaagtaa aatagtagta tcaaaaacta ctcatcctga
    10861 aactttatca gtactaagaa cttacttaca atataaagat tgtgagatag tagagattga
    10921 cttttgtaat gaatatggga ctacagatat agaaaaatta aaagcttctg tagacaaaga
    10981 tacagcatgt gttcttatac aaactcctaa tttctttgga attattgaag aaatggaaga
    11041 aatagaaaag ataacccatg aaaataaggc tatgttaatt atgagtgtag acccaatatc
    11101 attaggagtt ttaaaaacac caggtgagat aggtgcagat attgttgttg gagaagctca
    11161 atcacttgga aaccctctta attttggagg tccttatgta ggatttttag ctagcaaatc
    11221 taaatatact agaaaaatgc caggaagaat agttgggcaa agtttagatg tagaaggtaa
    11281 aattgcatat gtattaactt tacaaactag agaacagcac gtaagaagag aaaaagcaac
    11341 ttctaatata tgttcaaatc aagccttaaa tgctcttgta gcttcaatat atatggctac
    11401 tatgggtaaa gaaggattta aagaggttgg tatgcagtct atgaaaaaag cacattatac
    11461 ttataataag cttgtacaaa ctggaaaata taagccaata tttaaaggta agtttttcaa
    11521 agaatttgct gtacaaggaa atcttaatat agaaactata aatgataaac tcttagaaga
    11581 aaatatatta ggtggttata atttagaata caattatcca gaattaaaaa attcaacttt
    11641 gctttgtgtg actgagaaaa gaagcaaaga ggaaatagat aagttagttg gaataatgga
    11701 gggattataa tgaaagaata caatagtttg ttgatagata tatctaaaaa aggtagaaaa
    11761 gcatactcac ttccaaagtt agatatagat gatataaaaa tagaagatat gattgaccaa
    11821 gatatggcaa gacaaagtga gttgaatttg ccagaagttg gtgaattaga attagttaga
    11881 cattatacat tattatcaaa taaaaacttt ggagttgaca caggttttta tcctttgggt
    11941 tcttgtacta tgaaatataa tcctaaaata aatgaagata tggcagcact tccaaacttt
    12001 actggaatgc atccgtacca atcttcggat acagctcaag gttctctttc tttgatgtat
    12061 gatttatcaa gaagacttgc agaaattaca ggtatggatg aagttacatt acaaccttca
    12121 gcaggttcgc atggtgagtt tacagggctt atgattataa aagcatatca tgaaaataga
    12181 ggtgaccata agagaaccaa agtgataatt ccagattctg cacatggaac aaatccagca
    12241 agtgcagcta tggcaaactt tgatgtgatt caaatagcat ctgataaaaa tggagctgta
    12301 gacataaatg cattaaaaga ggttttaaat gatgaagtag cggctcttat gcttactaat
    12361 ccaagtactc ttggtttatt tgaaaaaaat ataaaagaaa tagctactct tgtacatgaa
    12421 gcaggaggac ttttgtacta tgatggtgca aacatgaatg ctattatggg gataacaaga
    12481 cctggagata tggggtttga tgtggttcat cttaatcttc ataagacttt ttcaactcct
    12541 catggaggtg gaggtccagg agctggtcct gttggagtta aaaaagagtt agtacctttt
    12601 ttaccaatac caataattga aagaaaagaa gataagtatg tactaaatta tgatagagaa
    12661 aaatctattg ggaaaattaa aaatttctat ggtaattttg gagttcttgt aagagcttac
    12721 acatatatac taactatggg aagggatggg cttaaagagg ctagtgaaat ggcagtttta
    12781 aatgctaatt atataaagga aagtataaaa gatgattata ttttaccaat agataccttg
    12841 tgtaagcatg agtttgtatt aggtggattg ccaagaggag aggccaatat aaaaactatc
    12901 gatattgcaa aaagactact agattatgga taccatcctc caacaatgta ctttcctctt
    12961 attataaatg aggcacttat gatagaacct acagaaagtg agagtataga gacattagat
    13021 agttttatag aagctatgaa aagtgttgct aaagaggcaa aggaggaacc agaattattg
    13081 aaaactgctc cacataatac tttggtcaaa agagtagatg acgcaagggc agtaaagaaa
    13141 cctatactaa catggtcaca aagataaata attaaaaagt agagatttaa agtataaaga
    13201 gaattaaatt tataagaaaa agctatctaa cttaatctag tttagatagc ttttttgtat
    13261 attagtatgt atggtatttt ctatataaag ttttaaatta tattagtgct tatatagaat
    13321 attgatgtga tgattatgta tagatattca taagaattgt tgaatttatc ttgctttaac
    13381 tttatttact cttttatatt aagaaaaact taactaaaat atagcgaaat tcataaaaat
    13441 ttaggaaatc ttaagaatta taatatttaa atctttagat ttatatttta ttatataaac
    13501 tatagaataa taaaataaaa aaattgagaa ttttataata tatttgtaga tttgagtata
    13561 aaaaagtgct aagttgaaag gagtaaaata tggataaggt taatttagaa aggtatagtt
    13621 gtgatataaa tgttggttta aataaaaatc aacttcaaag tagaatagat gataatttaa
    13681 ttaatgcatt tgaaaatgga aaaaccaaaa catataaaca aatttttaaa gacaacatat
    13741 ttacactttt taatctcata aatattgttt tagtctgttt attagtttca gttggttctt
    13801 ttaaaaatac cttatttata tttattgctg taattaatac tattatagga gtaatacaag
    13861 agataagagc taaaagatta ttggacaatc tatctgttat aatttcaaat aaggtatctg
    13921 tcataagaga aaaagagaaa aaagagattg atgtacaaga attagtatta gatgatatta
    13981 tgattttaaa aacaggtaat caaatctgtg cagattcaaa agttttaaat ggaaaattag
    14041 aggtaaatga gtctttggtt acaggtgaat ccgatattat tataaaaaat aaaggcgatt
    14101 ttttatattc tggtagtttt gtagtttctg gagaagcttt tgtaagagtt gaaaatatag
    14161 gaaaagataa ctatgcaaat aagataacta atgatgcaaa gatttctaaa aaacataatt
    14221 ctcaacttag aaattcatta aatgcaatat taaaaattgt aggtataata ataattcctc
    14281 tgggaattat attgtttgca aagcagcatt ttggaaatgg agattctata gctacatctg
    14341 tagtaggtac agtggcagct gtaaatggta tgataccaga gggattaaca ttacttacga
    14401 gcattgctct agctgtagga actataaaac ttgctagtca taaaacttta gtgcaagaac
    14461 tgtactgcgt agagactctt gctagagttg atacactatg tcttgataag acaggtacaa
    14521 ttacagaagg taaaatgcaa gttgatgata tagtaatgtt ggaagaagtg gatattaatg
    14581 aaataatggg gaatatatgt aattctctca aagatgataa tgcaacacta aatgcaataa
    14641 gagataagta taatgtttta gatacgtaca aagctaagaa tttaatacca ttttcttcag
    14701 aaagaaaata cagtggggtt acctttgaaa ataaaggtac atatttatta ggtgcatttg
    14761 agtttatatt taaggaaaaa aataaaaaac ttagaataga agttgaaaga tattctaaaa
    14821 aaggaaacag agtaatagtt ttagctcata gtgattctta tatggaagat aataatattc
    14881 ctgaagatat aaagccacta gcttttatat tgatattgga taaaattaga gatgaagcta
    14941 aggaaacttt agaatttttt tacaatgaag gtgtagatat aaaaattata tctggtgaca
    15001 atcctataac agtaagtgag gtagctagaa aggcaggtct taaaacggct agtaatttta
    15061 ttgatgcaac aacacttaaa aatgatagtg atatatacga agcagttgat aaatacagtg
    15121 tttttggaag ggtaagccca caacagaaaa aacaaatgat tttagcatta aaaaaacaaa
    15181 atcatacagt agctatgact ggagatggtg taaatgatgt actagcactt aaagaagcgg
    15241 attgtagcat tgccatggca tctggaagtg atgttactaa gaatgtttct aatttagtct
    15301 tattagattc aaactttgca tcaatgccaa aggttgttat ggagggaaga aaagtaatta
    15361 ataacattca aagagcatct tctttgtttt tagtaaagac aattttttca tttttcttgt
    15421 cgttattaac attatttttc ttcagtagac agtatccatt tgttcctata caattatcac
    15481 tcattggggc tgtaacagtg ggtataccat cattcttctt agctcttgaa gcaaacaaaa
    15541 gtagaatttc aggaaacttt ttactcaata tattaaaaaa tgctttacca ggagcactat
    15601 gtgtgacagt aaatgtaatt attgtttaca atatagttga atatttaggt tatagttcaa
    15661 gtgtattttc cacaatgtgt gctatattaa ctggtgtatg tggtctttta atattaagac
    15721 aacaatgttt gccatttaat aaagaaagat tatttttaat tgtaagtatg actattgcat
    15781 ttgtcattgg gattctattt ttaggtggat tattctcatt tgtagcattg agtagagaat
    15841 taattctcat aactatggca ttagcaatat taatgccaat tatgataaaa gttatgttat
    15901 ttgtcttgga tgagatatta gagacaattg ttccagattt aaattaattt taaaatgata
    15961 taaataagag cagttttagt tgaactgctc tttttagatg tgtttaattt tatgtaagaa
    16021 tatgtatttt tattaatcag ctatatatga gcaacagtag tcaacaactt cattaacttt
    16081 taagtcaaaa cttgaatcgc taggaacatt aaatttttga ccttctttat aagttacaaa
    16141 ctcattgctt ccaggcagtt ttacatccat acttccagca agcatttcca ttatttcttc
    16201 ttctctagta gaaaaagtat attctcctgg caacattaat cctaaagttt ttctttctcc
    16261 attaggcagt attattactc tgctacttac cttaccatca aaataaacgt ttgccttctt
    16321 tactgcagta acattcttaa attcattcat tagataaaac ctccaaaatt atttgtacat
    16381 atatattata taaaaataat gtaaaaaata ctatatattt acaaaaaaat ttagtattta
    16441 ttaaaaaaat taataaaaat aaaaaagaga tgaaattgct taaattgata aaaaatataa
    16501 aataaattgt aaagtaacta aga
//
LOCUS       DAEMAE010000075        16476 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEMAE010000075 DAEMAE010000000
VERSION     DAEMAE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.076"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            63..725
                     /locus_tag="KPH93_001886"
     CDS             63..725
                     /locus_tag="KPH93_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688706.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKIEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            1095..2504
                     /locus_tag="KPH93_001887"
     CDS             1095..2504
                     /locus_tag="KPH93_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG1688707.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVNLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNRDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            2619..2894
                     /locus_tag="KPH93_001888"
     CDS             2619..2894
                     /locus_tag="KPH93_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG1688708.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSIGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            3523..3834
                     /locus_tag="KPH93_001889"
     CDS             3523..3834
                     /locus_tag="KPH93_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688709.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            3836..5761
                     /locus_tag="KPH93_001890"
     CDS             3836..5761
                     /locus_tag="KPH93_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG1688710.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVDFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVVDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            5764..6255
                     /locus_tag="KPH93_001891"
     CDS             5764..6255
                     /locus_tag="KPH93_001891"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG1688711.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            6307..6870
                     /locus_tag="KPH93_001892"
     CDS             6307..6870
                     /locus_tag="KPH93_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG1688712.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            6885..7862
                     /locus_tag="KPH93_001893"
     CDS             6885..7862
                     /locus_tag="KPH93_001893"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG1688713.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYSKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            7849..8172
                     /locus_tag="KPH93_001894"
     CDS             7849..8172
                     /locus_tag="KPH93_001894"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG1688714.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            8289..10067
                     /locus_tag="KPH93_001895"
     CDS             8289..10067
                     /locus_tag="KPH93_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG1688715.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            10064..11437
                     /locus_tag="KPH93_001896"
     CDS             10064..11437
                     /locus_tag="KPH93_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG1688716.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            11443..12111
                     /locus_tag="KPH93_001897"
     CDS             11443..12111
                     /locus_tag="KPH93_001897"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG1688717.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            12388..13437
                     /locus_tag="KPH93_001898"
     CDS             12388..13437
                     /locus_tag="KPH93_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1688718.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMVELKK
                     DGALEAYKPKNYDKVDKKFKDTEDNVSWVGMSVPETAIVVNYKELKKLGIDVPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            13527..14516
                     /locus_tag="KPH93_001899"
     CDS             13527..14516
                     /locus_tag="KPH93_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1688719.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGAIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKATKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            14521..16239
                     /locus_tag="KPH93_001900"
     CDS             14521..16239
                     /locus_tag="KPH93_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG1688720.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTIFVSVISTVISLALALVYAYALTR
                     TNIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLITNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGIVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(16320..>16476)
                     /locus_tag="KPH93_001901"
     CDS             complement(16320..>16476)
                     /locus_tag="KPH93_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1688721.1"
                     /translation="VEENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKR
                     KINKQYQ"
BASE COUNT         6339 a         2009 c         3083 g         5045 t
ORIGIN      
        1 tactataatg tatagatgat aaaaacttta acaaaaattt agtgtatagg agttgtttaa
       61 gtatgataga tgtaagtatt gatgacaaac ttaaggaaag atggcctaat gctaagctgg
      121 gttgcattca agcaaaagta gcagtaaata aaagctcaga aaaattaatt aatgaaataa
      181 atgagttttg tgatactata aatcaaagtc taaaaattga agacattaca aaactagata
      241 aaattaaaga tgcaagaaat gcttataaag agcttggaaa aaatccaagt agatatagaa
      301 cgtcttcaga agcactggtt agaagaatta ttcaagggaa gggtctttat acaataaaca
      361 atatagtaga aataaacaat ttaatatcaa taaaatcatt atatccagta ggtacatata
      421 atgtaagcaa attacattca ccagtatgtt ttactgtagg agatgaaggg gaacagtata
      481 aggggattgg gaaagaatta ataaatatag aaaatcttcc tatattgtcc gattcagtgg
      541 gtaaatttgg gagtccaact agtgattctg aaagagcaat gataacagaa gatgcaaatg
      601 aaatattaat ttgtatattt tcttttagtc aagaatctga tatcgaagaa tattttgggt
      661 atgctaagga gttacttaaa gaatatgctg atggaaaaga tatagaaact aaaataatta
      721 aatagaatct atataacaac gttatgttaa gctaaaattc aatataaaaa taagttaaga
      781 taaagaaact aacataaaat tagtttcttt tttaaaagag tagtttagtg ttataaaaac
      841 agatagataa cccacgatta ttaaaagtat ggtaatatat taatatagta gagcttgtat
      901 ttaagggatt ttgaaagggg tgatttataa taatcatttt atctaaaaga taaaatggtt
      961 ttatggaaat aactatatat ttaaaaatat aaataatgat acagtaattt gggtatataa
     1021 aataagacat acatggtttt gtttaaaaac caataaagta catatgtaat tacaatgtaa
     1081 ggaaggattt aattatgatt gatggaaaag aggtaaaaca agaaagtgga caaaaaaatc
     1141 taaaatttaa gatagctata gtagtaggtg ttttgtttgt agcttatata ggaatggcaa
     1201 tcttttttag aaatcatttt tactttggaa cgactattaa tggagtaaag gcttcaggaa
     1261 aaacagttga gcaagtaaat gatttgttaa cgtctagtac taattcctat actttaaact
     1321 tagaggaaag aaacaataag aaagaacaaa taaaagcaaa agatattgat ttaaaattta
     1381 caccagatga taaagttcaa aagataaaag atagtcagag cgctattgga tggattggtg
     1441 gaatatttaa gtctaaagag tataataata tggtgaatct ttcatataat aaagatttat
     1501 tagaaaaaag atttaacgct ttatcttgct ttgatagtaa aaatgttgta gaacctaaga
     1561 gtgcttatcc taagtatgat agcactaaaa atgcttatgt tatagtgcca gaggtacttg
     1621 gaaacaaagt gaaaaaagat gatttttata agttagtaca aaactcaatt aattctggga
     1681 atacttctat ttctctggat gaaagtaaag tgtatgaagc accaaacaac acaaaggatt
     1741 cagaaaagtt gaaacaagct attaagacac tagataagta tgttggcgta gtcgttacat
     1801 atgattttag tgaccgtaag gaaactttag atggttctga aatcaataaa tggttaaaag
     1861 tagactcaga aaagaattat gacataactt ttgataatga tgctatggta gaatatacaa
     1921 gaggtttaag taaaaaatac agtacttttg gagactcaag accatttaaa actgccacag
     1981 gtaaagatat tacaatttcg ggtggaattt atggttggtt gatagacaaa aagaaagaag
     2041 ctgaagcatt agttgatgtt gtaaaagctg gtaaaaatgt tacaagagaa cctatttaca
     2101 tacaaaaagc tgtatctcgt aatagagatg atattggaaa tacatatgta gagattagtc
     2161 tttctggcca acatatgtgg ttctttaaaa atggagaaat gcttgtatca acagacgttg
     2221 taacaggcga tttagctaga ggatttgcta caccatcagg tatatatcct cttaattata
     2281 aggctagaaa cgtttcatta actggtcaag gttacagttc acctgtaagt tattggcttc
     2341 catttaatgg gaacataggc attcatgatg ctacttggag aaattctttt ggtggaggta
     2401 tatataagtc ttcaggttca catggatgtg taaatacacc tttttctaag gcaaagaaaa
     2461 tatatgaaaa tatagaacca ggtacaccaa ttattttata ctagtaaatg tgttgctaac
     2521 agttaagcga agtcatctta gtttatttag gatggcttcc tttttatata aagcttattg
     2581 aagtttacat aaagactatt taaaaggaga aatacataat gaatgaaaag ttaactaaaa
     2641 ttttagaaga agctaagaat gatgatgaat ttagaaagga acttataaaa acaagagaac
     2701 aacgtgatgt tatggattca ttttgtaagg ttgttacatc tagaggttat gatataacag
     2761 taggggaact tttttccata ggagaagaat atacatccaa tctactgaaa agtgtaaatg
     2821 gaggagcagc atatcctatt gaagattgga gtgattggta cgaggatttt tttataatgt
     2881 tgtcgaatat atagagtaat aatgatatta tctacttaag attaatacat tgccttaaaa
     2941 taaaactatt agaactaagt gtaactttta tcaatcttta aaaatattga tttatttcta
     3001 actagatata aaaaatttag attaaaaata aggtgacaaa aatcgatata gcttgatgat
     3061 ttataaaagt tagataaaat agtgcgagat atttctaaaa attcaaaaaa aacaataaac
     3121 ttcaaaggtt agaggttgaa aaagaatata aaaaactttt atatgtaata aattaaattg
     3181 aaaatataaa aaaaataaaa taaaaatttg aaatgtcgac ataacgttaa ttttaaaaca
     3241 aaataacgct attgacaaac tatatcgctt taaattataa ttattgaaaa taatggtatt
     3301 taaaaaccat actcatgtca tagtctaaaa gtaagtcaaa aaaggatgta aatctaggca
     3361 agtactattc taaaatatgt tttattaaga aataaacgtt gaaaaatatt aattattata
     3421 taaaaatgct gtttatactt taacaatgag gtagtaatct aattagaata aaaaaatcta
     3481 attggttata tacatagtaa aaaggtaaga gaggtgattt ggatggcttt tgaaataata
     3541 aaaagcattg ttgaggcaga gcagacagca gacagtatca aagtaaaagc tgttactgat
     3601 gcagagtcta tcagagctga tgctgtaaac aaatgtgaaa gcatatttgc tgatgtaaaa
     3661 aaacaagcaa agcttatgga agaaactctt attgagaagg cagtcaccga cagtagagca
     3721 gaggttgata aaatcttagc taatgctaaa agtgaatgtc tgaaaattga aaaaactgct
     3781 gaagaaagaa aaagtaaggc tattgaagcg gttattggaa aggtagtgag ataatatggc
     3841 aatagttaat atgaacaaaa tatctattgt tggcttagaa gctcaaaaaa gccagatttt
     3901 aaagctactt atgaaccgtg ggtttgttca gattgatgat agtgcctttc ttactgagga
     3961 ggaggaatta aaaaattatc ttgtaaaaga tagtgaagaa tctgaagtta taatgctaga
     4021 gcagaaaatg tctcatgtaa accaagcaat taatattgtt tcttcactag tgaaacaaaa
     4081 aaaatcccta cttgcaccaa aaagagagtt taacaaaatt gatagtgaaa aagaagggca
     4141 agtttatgct gaagctctgg agttaaacaa tttgcacaag gagataggta caataaggtc
     4201 taacataaac ttacttaaca acaataaaag tatgttagaa ccatgggtag actttgatat
     4261 tcctcttgaa aatatggaaa ctaagtatac aaaaactata cttggaactg ttcccataag
     4321 cgcaaacatt gatgctttaa gagtaaaatt agaagatgaa ataccagaaa gtgttattgg
     4381 gattgtaaat tctgacaagt taatgaacta tgtttactta attactttaa aagaaacttt
     4441 tgatgatgtt ttagaggttt taaaagaatt taatttttca gtagtttcat taccaaatga
     4501 agaaggtaca ccgttacaag ctatcaagaa atacaatgaa atgataatta gacaggaaaa
     4561 cgtttctaag gacaaaacag aggaaattaa aagacatgca gatagtatac acatgcttga
     4621 aaacctatat gatttttaca ctatagaaag agaccaaaaa aagattatag agagacttgt
     4681 caaaacaaaa acaacattct gtcttaatgg ttggttacca agcaaaaggg cagatgaatt
     4741 aattaaagaa attacagaaa agtttgattg ttgtgttcag actgaagaag gaagtaaaga
     4801 agaaggcttc ccaattctgc ttgaaaacaa taaattagta acaccttttg aaagtgtaac
     4861 taacatgtac agctatccaa gtacaaaaga tattgaccca aatacaattt taactttctt
     4921 ttttgttgta ttctttggaa tgatgctaag tgacgctgca tatgggttaa taattgcagt
     4981 agtctgtgga tttgttgtat ataaattaaa gatacaaaaa ggtgaaggaa atttaattaa
     5041 acttattggt atctgtggag tttcaactac tgtatggggt cttatattcg ggggtatttt
     5101 gggggattta attcctataa aagcattaat taatccttta gaggatgtta tgacacttat
     5161 gggaatgtcg ttactgttcg gtattatcca tatatatgta ggtttgggaa taaaagcata
     5221 tacattaata agaagtggaa aggtagtaga tgcaatcttt gacataggtt tttggtattt
     5281 gtgtataaca ggtgtatgtc tacttataat accttttgtt gcaggggaca taggtgtatt
     5341 ttcagaagta ggaaaatatt tagcaattat aggtgctata ggtcttgtac ttactcaagg
     5401 aagaagcttt aaaggtattc ctgttaagct ttttaaagga ttttcaagtc tttatggcat
     5461 aacaagttat tttgctgata tattatctta cacaagaatt atggcacttt gtttaacaac
     5521 aggagttata gcacaagtta tcaacctttt aggggctata gctggtccaa tacttgcagt
     5581 tgtaatcggt gttgtaggac acacaataaa ccttcttata aatgcactag gtgcatatgt
     5641 tcatacaagt aggttacagt atgtagaatt tttcaataag ttttatgaag gcggaggagt
     5701 tccttttgtt ccgtttaagt ataaaacaaa atatacaagc attaataaaa aggagatgta
     5761 aatatggaat ttttaaacgc gttaggaaat ggtcaatttt tggcaatatc aggagctgct
     5821 atagcagcat tatttgcagg tatgggttct gctaaaggta cttcaatagc aggtcaagcc
     5881 gcagcaggtg ttgtaacaga agaccctagt aaattcggtc aacttctagt tttacaactt
     5941 cttcctggta cacaaggtct gtatggattt atcatagctt tccttatttt atcaaaagca
     6001 gggattattg gtggagatgc aataccaaca tcagctcaag gtttacaact tcttatggca
     6061 ggacttccaa ttggtttagt tggtttagta tcaggtatag cacaaggtaa agcagcagca
     6121 gcaggagttg gaatagttgc aaaaagacct gaagaacttg gtaaagctat aacatttgca
     6181 gcaatagttg aaacatatgc tcttttagga ctacttgttt ctttcttagc ttacaatgga
     6241 attgcaattg gttaattata aattataatt cataagtaaa aaaaatgaaa aggaggaagc
     6301 actcctatgg gtaatgaaca aaaaatgatt gacagaatta tagctgatgc caaacaagaa
     6361 gcacaagaaa tattggataa agctaaaagt gaagcagatt taaaagtaaa ttcagctaat
     6421 gaaaaagctg aaaaggaaat ggcttcctat acaaagcttg cagaggcaga agcagaaaaa
     6481 gcggcttcta aggaaatatc aggagcttat atggaggcta aaaagcaaat attatcaaaa
     6541 aaacaagaga ttttagaaga ggtaatttta gaagctaaaa acaaactttt aaatcttaaa
     6601 gataatgaat atgaagagat tatactaaac atgattgaaa aatcaaattg tacagatgat
     6661 tcagagatag tattatctaa aaaagataaa aaaacattaa aagatgtgct atcaaaaaaa
     6721 ggcattaaag tgtctgatga aactagagat attactggag gtttcatagt taaaaaaggt
     6781 gacattgagt acaattattc atttgaagcc ataattgcag tagagcatga atatattgaa
     6841 caaattgcag cagaaatttt gtttaattaa ggaggggtta aaaaatggca gaagaaaaaa
     6901 cttatcccta tgccgttgca cgaataagag tgcttgaaaa acagcttctt aacagacaaa
     6961 tgtttattca aatggcagaa gccaaaagcc ctgaagactc tttaagaatt atagctgagg
     7021 caggttatgc tgatacatca aataataata tacataattt tgagaatatt ttgactcaag
     7081 aattatcaaa gacatatgaa attcttaaaa gtctagctcc tgaagaaaaa tttgttgatg
     7141 tatttcttta taaaaatgac tatcacaatt taaaagtgct gattaaagaa gaaatttctg
     7201 gtgtagatgg tgagaaatat ttaattgatg gaggtacaat tcctcttatt aagcttagag
     7261 aatctttagc aaatcgtagt tttagtgatt tacctaagat aatgtcatca gctatattag
     7321 aggcatttga tgtttatagt aagacacaaa atggccaaat ggttgatatt gttctagata
     7381 aagcaacctt taccagtatg aaagaaactg ctaaagaaag ccaaaacaaa tttgtaatag
     7441 attatgttat gaaagtttgt gatttaacaa atctaaagag ttttataaga gtcaaaaata
     7501 tgaagaaaga ttttgatatg tttatgaatg tgtttgtttc aggtggctca cttgataagg
     7561 aaaaattctt agaagctttt tcctcagata ctccagcatc atgttttaaa tctacttctt
     7621 actctgatgt ctgtaaaaat ggcatggata gtggttttac tgtctttgaa aaactttgtg
     7681 atgactatct tatggaatat gttagaggag caaaatttaa gcctcttaca ttagaaccac
     7741 taattgcata tttatatgct aaagaatcag agataaaaac aattagaatt attttgacta
     7801 gtaagcttaa taatatagac gcagatacta taaaagaaag gctaagggat gcttatgtat
     7861 aaggtaggag tagttggaga taaagatagc atcatgggat ttttagctct tggaatagat
     7921 atcttcccag cctatgatag tgacgaaatt aaaaaaagta tacataagtt ggttgaagat
     7981 gaatatgcga taatttatat tacagaacag gcatcattgc ttgcaaaaga atcaatagca
     8041 aaatacaagg attaccaact tcctgccatt atagttattc caggtattgg aggaagtatg
     8101 ggattaggta tgaatgaagt aagagaatct agtaaacgtg ctataggtgc agatatatta
     8161 tttaaagaat agatgaaagt tgaataaaag acaatctcaa attttattgg tgctaaatat
     8221 atgtactaat aaaatatagg agggaatatt taaattttga taaagtatat gattgcaggt
     8281 gagagaatat gaaaacaggc acaattgtaa aggtgtcagg accgcttgtt gtagcggagg
     8341 gcatgagaga tgcgaatatg tttgacgtcg taagagtttc agataaacat cttataggcg
     8401 agattataga aatgcatgga gataaagctt ctatacaggt ttatgaggaa acttccggtc
     8461 taggaccagg ggaagaagta gtttctcttg gaatgcctat gagtgttgaa cttggaccag
     8521 gtcttatttc aactatttat gatggtattc aacgtccact tgaaaaaatg tatgatattt
     8581 caggtactaa tataactaaa ggtgttgaag tatcttcact tgatagaaca actaagtggg
     8641 aatttaaacc taaaaaatct gttggtgata aggttgtagc aggagatata attggtggcg
     8701 ttcaagaaac agcagtagta gaatgtaaaa ttatggttcc ttatggagtt aagggtacta
     8761 taaaagaaat atattctggt gaatttactg ttgaagatac agtttgtgtt attacagatg
     8821 aaaaaggtaa tgatatacct gtaactatga tgcaaaaatg gccagttaga aaagaaagac
     8881 catatagaga aaagaaaaca ccagattcac ttcttgttac aggtcaaaga gttatagata
     8941 catttttccc aatcacaaaa ggtggggtag cagctatacc gggacctttt ggaagtggaa
     9001 aaactgttac acagcatcag cttgctaaat gggcagatgc agatatagtt gtatatatag
     9061 gctgtggcga gcgtggaaat gaaatgacag atgttcttct tgaatttcct gaattaaaag
     9121 acccaagaac aggcgagtca cttatgcaaa gaactgtgct tatagcaaat acatcagata
     9181 tgccagttgc tgcacgtgaa gcttctatat acactggtat tacaatagct gaatacttta
     9241 gagatatggg atatagtgtt gcacttatgg cagactctac atcaagatgg gctgaggctc
     9301 ttagagagat gagtggtcgt ttagaggaga tgcctggtga agaaggttat cctgcatact
     9361 taggttcgcg tcttgctcaa ttctatgaga gagcaggaaa ggtaaattgt ctaggtatgg
     9421 atgaaagaga aggaacactt acagcaattg gtgcagtttc tcctcctggt ggtgatacat
     9481 cagaacctgt cagccaagca acgcttcgta tagtaaaagt attctggggt cttgatgcat
     9541 ctttagcata taaacgtcat ttcccagcaa ttaactggtt gactagttat tcactctatc
     9601 aggaaaaagt tgatgtatgg gcagataaaa atgtaaatga taatttctca gctcaaagag
     9661 ctcaagctat gaaactttta caagttgaat ctgaacttca agaaatagtt caattggttg
     9721 gtgttgattc actttcagat ggagatagat tgatactaga ggttactcgt tctattagag
     9781 aagacttcct tcatcaaaac tctttccatg aagttgatac ttatacatca cttcataaac
     9841 aatatagaat gatgggactt atattaaaat tctataagtc agcacaagat gctatcaagc
     9901 aaaatgttac tataaacgat attatcaagc tttcagttct tgaaaagatt ggtcgtgcta
     9961 agtatgtaga agaaggcgat atagatgcag cttacgattc aattgaagat gaaatagaca
    10021 acgagcttgc agaccttatt aaaaagaggg gggctgaaga actatgataa aggaatataa
    10081 gtcaatacaa gaggtagccg gtcctcttat gttaataaac ggcgtagaag gtgtaaaatt
    10141 tgatgaactt ggagaaattg aactttcaaa tggagaaatt cgccgttgca aagttctaga
    10201 ggtaaatgga gatactgcac ttgttcagct tttcgaaagc tcaacaggta taaaccttgc
    10261 tgaaagtaaa gttcgttttt taggaaagag tcttgacttt ggagtttcac cagatatatt
    10321 aggtcgtgtt tttagtggaa tgggtagacc tattgatgga ggtccagaaa ttataccaga
    10381 taaaagactt gatataaatg gagctccaat aaatccagca gcacgtgatt atccggccga
    10441 gtttatacaa actggagtct ctgctataga tggacttaat acattggtta gagggcaaaa
    10501 acttcctata ttctcagggt ctggtttgcc acatgccaat cttgcagttc agattgcaag
    10561 acaggcaaaa gtacgtggaa ctgattctaa gttcgccgtt gtatttgctg cagttggtat
    10621 aacttttgaa gatgctgaat tttttataag cgattttaaa gcaactggtg caatcgaccg
    10681 ttctgtagtg tttgtaaacc ttgcaaatga cccagctgta gagcgtgtat ctacacctag
    10741 aatggcactt acagcagcag aataccttgc tttcgagcaa gatatgcatg tacttgttat
    10801 aataacggat attacaaact atgcagaggc attgcgtgaa gtttcagcag ctaaaaaaga
    10861 agttccagga cgtcgtggat atccaggata tctttatact gaccttgcta ctatgtatga
    10921 aagagcagga aaaatgaaag ggcatgaagg gtctataact atgattccaa tacttacaat
    10981 gcctgaggat gataagacac atccaattcc tgaccttaca ggatacatta cagaagggca
    11041 aattatactt agccgtgaac tttataaaaa agatatacag cctcctatag atgttttacc
    11101 atcactttct cgtctaaaag ataaggggat tggtaaaggt aagactcgtg aagaccatgc
    11161 agatacaatg aaccagcttt ttgcagctta ttcaagaggt aaggatgcaa aagaacttat
    11221 ggctatactt ggtgaatctg cactttctga gattgattta atgtatgcta agtttgcaga
    11281 tgaatttgag aaagaatatg tttcacaagg atttagaact gatagaacta ttgagcagac
    11341 attagaaatc ggatggaagc ttcttagtat gttaccaaaa agtgaactta aacgtattcg
    11401 tgatgaatat tttgaaaaat atatgcctaa ggagtgatat ctgtggctag attaaatata
    11461 aatccaacac gtatggaaat gactagactt aagaaactcc ttaaaactgc tacaagaggt
    11521 cataagcttc ttaaagataa acttgatgaa cttatgaaac aatttcttga gattgtaaga
    11581 gaaaataagc gattacgtga agaagcagaa aatgctcttg atactgctta taaaaatttt
    11641 ataatcgcaa gagcagttat gagtcaggag tatctgggtt cagcacttat gatgccaaag
    11701 caatcagttt cagtagatgt ttctacgaga aatataatga gtgttgatgt tcctgtgttt
    11761 gactttaaaa ctgaaaacaa tcaaagtgat atttatcctt atggtcttgc ttttacatca
    11821 ggtgaattag attctgctat ggaagctttc tcagatgcta tgcaaccact tttaagactt
    11881 gcagaaagtg aaaaaagtgc acagcttctt gcacaagaga tagaaaagac acgtagacgt
    11941 gttaatgctc ttgaaaatgt aatgatacca aattacattg agactattaa gtatatagct
    12001 atgaagcttg aagaaaatga gagagcaagt actactagac ttatgaaagt taaggatatg
    12061 gtgcttaaaa aagcacttga agaaaagaaa aagaatgatt tggttgtata attaaatact
    12121 tataaaaata agtctaacat tctactttat agaggttaga cttatttttt atgtttaaat
    12181 taaaatgtag atattataaa aattaatata ttgtttaaaa acagactgta tttaattaaa
    12241 aataatatat ttaacaaaaa tttctaaaaa ttaacgaact gttaatttac acttaacttt
    12301 atattaacaa attaggtgta ttatataaac ataagcaaaa acaataaaaa acaaataaaa
    12361 aaaggaaaaa acacaaaggg ggaagatatg aaagaattta tatttacttg tctgtgcatt
    12421 gtagtgatta catttatgac aagttgcata tcaacaacac cacaaactac tcaaggaaaa
    12481 ggaaataata aacttacaat atatgcagca ctagaggaag agcagatagc ttattatatg
    12541 gaaggtttta gagaagagta tcccaatata gatgtagaga tagttatgga ttctcatggt
    12601 gtaatagctt caaaactatt agcagaaaaa gataatccac aagcagatat tatatgggga
    12661 ttatcagcaa tgaacatggt agagctaaaa aaagatggtg cacttgaagc atataagcct
    12721 aaaaattatg ataaagttga taaaaaattt aaagatactg aagataatgt atcttgggta
    12781 ggaatgagtg tcccagagac agcaatagta gttaattata aggaacttaa gaaactaggt
    12841 atagatgttc caaaaagtta tgaagattta ataaaaccag aatataaagg acttataaca
    12901 atgccaaatc cggcatcatc tggaacaggg tatcttacag tatcagggct tattcaaatg
    12961 atgggtgaaa aagatgctta caattacatg gataaattac ataaaaatat agctacatat
    13021 acacactcag gttcaaaacc tgcgaagtta gctggctctg gagagtatcc aataggtatt
    13081 acacttggat atagagctac acaacaatta tctagtggag aacctataga ggttgtattt
    13141 ccaaaggaag gttcaggttg ggacttagaa gcaaatgcct tagttaaaaa agataatata
    13201 aaaaaagaag caaagttatt tttagattgg gcaataagtg attcagctat ggatagatat
    13261 gcacatgaag tagcagttac ttccataaag acagataatc cagttccaaa aggttattct
    13321 aaacaaccat ttgaacagct tataaaagaa gttgatttaa actctgctgc caaaaacaga
    13381 agtacagtac ttaaaacttg ggaatcaaaa tacggttcaa aatctgaaag taagtaaagt
    13441 ttaaattaat aatttagaga aaagaagttt agaagaaaaa gtaggatata aaatttaaaa
    13501 tataattata taaggagaga taagatatga gttacttaaa aataaataat gtgtttaaaa
    13561 gttatgacca aaaaagggtt ttaaataata ttagtttgga tattgaagaa ggggaatttc
    13621 tatgtttact cggacctagt ggatgtggta aaactacatt acttagaata atagcaggtc
    13681 ttgaagatgt aaatagtgga gctataattc tccaggataa agatattaca aatttagagc
    13741 catcaaagag aggttttggt atagtatttc aatcatatgc attatttccg aatatgactg
    13801 cttataacaa tatagctttt ccactaaaag agagaaaagt ttcaaaagaa aaaatagata
    13861 ataaagttaa ggaagtactt gaaacagttg gattgactaa tgaagctcat aaatatccta
    13921 aagcattatc aggtgggcaa caacagagga tagcaatagc aagagcatta gcacttgaac
    13981 caaagttttt acttttagat gaaccaatgt ccgcattaga tgcaaaggtt agacacaagc
    14041 ttagaatgga tattaaaaga ttacaaaaag aacttaatat aacgacaata atggtaacgc
    14101 atgaccaaga agaagctata actatggcag ataaaatagc tattctaaat ggaggagata
    14161 taatgcaaat aggaactcca gaagaaatat accaaaatcc tcaaaatcta tttacagctc
    14221 aatttatagg agatacaaac tgttttgata atggagattc tatattaact gtaagaccag
    14281 aatatgttca aatagagaaa gcgacaaaag aaaattacca agggataata tcaaatatag
    14341 agtttagagg gaatttatta agagttgaaa taaaagataa actaaatgaa aatttcataa
    14401 taagtgatgt ttctataaaa gagtgggtta atttaaattt agtagaaggt gaccttgtaa
    14461 aaataagtat agacgaaaaa tactatttaa agtatccaaa agttaaagga gcataagcgt
    14521 atgaataata atgcgataag ttctaaaagt ataagtctta aatcaaaaat gaatttagat
    14581 ataaaaaaat gtatgcttac agtattagtt atgtctttag taatattttt attggcacct
    14641 ataatatatc tatttgtaca ggctttttta gataaagatg gagtctttgt aggattacaa
    14701 aactttcaag aatacttggc ttctgaatct ctattaaatt ctctaaaaaa tacaatattt
    14761 gtttcagtta tatcaactgt aatatcacta gcacttgcat tagtatatgc atatgcattg
    14821 actagaacaa atattaaatt taaaggtttt tttaaatcat tagcaatgtt tcctatgttt
    14881 gctccaacaa tgatgcatgc aataggtcta gtgtatttat ttggaaatca agggctgata
    14941 actaatttac ttggagtgca tgttccaata tatggagcag tgggaataat attagcacaa
    15001 attatatatg tatttcctca atcatttcaa atactttata tatctctaag ttcaaccgat
    15061 tatagactat atgaagctag taatactatg ggaataagca agataagaga atttataagc
    15121 ataacaatcc caagtataaa atactcttta ataagttctg catttgtatc atttacatta
    15181 tgttttacag attttggagc tccaaaggtg ataggaggag agtttaatgt attatcaata
    15241 gatgtatata agcaaattat agggcaacag aatatgccaa tgggagcaac tgtatcaata
    15301 ttacttttaa tacctgcaat aatatcgttt atagtaacta aaaaaattga aaaaaatcaa
    15361 aattcatata taacttcaaa gtcaattcca tacaaggtta aaactaataa gttaagagat
    15421 agtattttag gagggattgt aggaattatt gcatttggta taatagctgt aatgttaact
    15481 gtaatcttat catcattaat atcagtttgg ccatatgatt taagacttac actggaacac
    15541 ttttctatga aaaacagtat tgatggaatt aaaccattta taaattcaat aaaaatgtca
    15601 ctattgacag ctattttagg gacgagcttt gttttcttat ttgcatattt aaatgaaaaa
    15661 acagaaaaag ccccatattt aaaatcattt gggtattttt taagtacatt gcctatggca
    15721 ttgccaggat tagttttagg tatagcttat gtattatttt ttaataaatt agagtttaat
    15781 tttatggata caatatacat taagaatttg tttaatggat tatatggaac attacttata
    15841 atggttatat gtaatatagt tcatttcttt tcagtagcat ttataacagc tactactgct
    15901 attaagaaag tagataaaga attggatatg gtagctaaat caatgggaat aaatagcgca
    15961 tcaattctta agaacgtaac tataccactt tgtttgccag cagttttaga gaactttatg
    16021 tactttttcc taaactctat ggtaacaata tcagcactag tttttatata tactgcaagt
    16081 tcaaaagtag ctgcaatatc aattgtacaa ctagatgata aggggagtac tgcacaggca
    16141 gcagcactag ctgtgttaat attagtcaca aatatattag tgaaagtaat atatgaattt
    16201 ataaagaatc gtttagaaaa aagaacaaac tttaattaaa taatttatct tataaaaaca
    16261 caaatttaaa aagtctattc ttatgattta ctatttaaag aatagacttt ttaaatgtat
    16321 tattgatatt gtttattaat tttcctttta ttatcataca tttgagaatc tgcacgacga
    16381 tatgtatcaa ataaagaaac atctactttt tcatcaaaaa tagcatatcc aatagacatt
    16441 gataatttta attcattagt tgagttttct tcaact
//
LOCUS       DAEMAE010000076        16460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEMAE010000076 DAEMAE010000000
VERSION     DAEMAE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.077"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..1279
                     /locus_tag="KPH93_001902"
     CDS             113..1279
                     /locus_tag="KPH93_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG1688722.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREVYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFVWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGVCIWSQNPEYVGFTLNVLERLARRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAADKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQVQKYFPVICGEWSLFNSYACGIDTDGGQSPLNGIESNIDKLSKDEKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            1301..2947
                     /locus_tag="KPH93_001903"
     CDS             1301..2947
                     /locus_tag="KPH93_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG1688723.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWITSLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFNSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRTPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            3044..4417
                     /locus_tag="KPH93_001904"
     CDS             3044..4417
                     /locus_tag="KPH93_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1688724.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYAEVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAILLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQDQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASVIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITVLQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            4865..5818
                     /locus_tag="KPH93_001905"
     CDS             4865..5818
                     /locus_tag="KPH93_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG1688725.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            5856..6818
                     /locus_tag="KPH93_001906"
     CDS             5856..6818
                     /locus_tag="KPH93_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG1688726.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIENIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            6843..7829
                     /locus_tag="KPH93_001907"
     CDS             6843..7829
                     /locus_tag="KPH93_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG1688727.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGVVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPDLEKAVVPRVDEIEEAIKSLIVR"
     gene            7842..8888
                     /locus_tag="KPH93_001908"
     CDS             7842..8888
                     /locus_tag="KPH93_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG1688728.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSNE
                     KKEMKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKA
                     LRAKLMDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            9055..10785
                     /gene="lpdA"
                     /locus_tag="KPH93_001909"
     CDS             9055..10785
                     /gene="lpdA"
                     /locus_tag="KPH93_001909"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG1688729.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYSSGNISKEDIV
                     EENQNTEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVEVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSFSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGMNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAAAFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            11203..14151
                     /locus_tag="KPH93_001910"
     CDS             11203..14151
                     /locus_tag="KPH93_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688730.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENTDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKQKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKYNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGKTNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSGEIAIPHTDGIDVIRPAI
                     SIAILKNPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            14129..14593
                     /locus_tag="KPH93_001911"
     CDS             14129..14593
                     /locus_tag="KPH93_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688731.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            14674..14976
                     /locus_tag="KPH93_001912"
     CDS             14674..14976
                     /locus_tag="KPH93_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688732.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKAFQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIVCTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            15038..16402
                     /locus_tag="KPH93_001913"
     CDS             15038..16402
                     /locus_tag="KPH93_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG1688733.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
BASE COUNT         6566 a         1766 c         3194 g         4934 t
ORIGIN      
        1 gaaaacggat aaaatcaaat ataataactg tatattaaaa taacttaaaa aaattatttt
       61 attataaatg caaaatatac catttaaata agataagagg ggaggacttt atgtgtatat
      121 aaaaggtgta aatttaggag gttggttagt tttagaaaaa tggatgacct caagtttatt
      181 tgaaggcaca gaagctgaag atgaatacta tttaccaaga caactttcaa gagaggtata
      241 tgaatctaga attaagactc atcgtagtga atacatcaca gagagagatt ttgcaacgat
      301 taaatctatg ggatttaatt cagttcgtat accagtacca tattttattt ttggagattg
      361 tgaaccattt ataggatgtg ttaaagaact tgataaagca tttgtttggg cagataagta
      421 tggacttagt atattgatag acttacatac agttccagga agccaaaatg gatttgataa
      481 tggaggaata tcaggtgtat gtatctggtc acaaaatcca gaatatgtag ggtttacatt
      541 aaatgtatta gaaagacttg caagacgtta tgggatgaga catgaattgt atggaatcca
      601 aattttaaat gagcctataa cagaaagaat gtggaacata atgaatgttc caaatcgatt
      661 taaggctgca gataaggaga tggcaagggg gagtaaacct aattcattag agtttttacg
      721 tgatttttat ataaaagcat ataaagtaat gcgtccttat atgagagagg aaaacgttat
      781 agtatttcat gatgcatttg aattaaaagc atggaaagac tttatgagag aggaagaatt
      841 taaaaatgtt gttctagata cacaccaata tttgatgtta gctgaagcag atggatgtga
      901 acaaagtatt gatagctatc tcaaatatat tagagaaaat tatgctaaag atattttaca
      961 agtgcaaaaa tattttcctg ttatatgtgg ggagtggagc ttatttaatt catatgcttg
     1021 tggtatagat acagatggag gtcaatcgcc tttaaatggg attgaatcaa atatagataa
     1081 attatcaaaa gacgagaaaa gagaattata taggaaaata gcaaaagcac agcttgatgc
     1141 atggagaaat ggaagtggtc actactattg gaattataag ttattactag atacagttaa
     1201 tgaagaagga tggataggat gggatagttg ggacctaggc aaatgtgtag ctcaagaatg
     1261 gtatcctata gaatattaaa ctaaaattgg aggaaaagat atgattaaaa accctatact
     1321 tccaggtttt aatccagatc cttgtatttg tagaaaggga gatgattatt atcttgtagt
     1381 ttcatctttt gaatggtttc caggtatacc tgtttatcac tctaaagact taaaaaattg
     1441 ggagctatat actcatattt taacagatga aacaaaaata gatttgaaga agcttccttc
     1501 atctaaagga atttgggcac cttgtttgac ttattgtgaa gaagaagatt tattttacat
     1561 agtttatgga attatgaact ctatgaatgc aagatatttt gatgttgata attatttaat
     1621 tacatcaaaa gatataaagg gagagtggag tgaaccagta tatctacact catcaggttt
     1681 tgatgcgtct atttttcatg atgatgatgg taaaaagtgg attacatcat tagactggga
     1741 gactagagag ggatatgaaa aacctggtgt tatatgttta gtcgaatact gtacaaaaaa
     1801 gaaagaaata gtaggatatc caaagagaat atggtcaggt ggaactgata gaggttgtat
     1861 tgaagcacca catataacaa agcgtggaga ttactattat ataatgtgtg cagaaggtgg
     1921 aactggatat ggccatagtg taactatggg aagagctaaa aatatatggg gaccttatga
     1981 aaaagattca atgaatccaa ttgtaacttc aattccaggt gatttttatg aaagacatga
     2041 ccctgaccat ctaaagccta aatactataa tccagaatca aaacttcaaa aatctggtca
     2101 tggaagctat atagaaacaa catcaggtga agtatattta gttcatctaa catctagacc
     2161 atttgtacca gagttaaggt gtactttagg tagagaaaca gctattcaaa aaatgaaatg
     2221 gacaaaggat aattggcttc gaatggaaga tgaaagtaat ttggcaaaag aatacgtatc
     2281 agaaagtaaa ctagaagaac atttagtttc atctatacca agttttgatg actttaattc
     2341 aaatgaatta ggtttacaat actatgctcc taggatttca ccattatcat ttgcagatgt
     2401 taagtctaga ccaggatatg taaggatcag aggacaagaa tcaagaacat cattaaataa
     2461 agtgagtata ttggcaagaa aattaacttc agtatatgca agaataacca ctaagatgga
     2521 gttttaccca gaggtacatc agcatagtgc gggtttgata atgtattatg ataatatgaa
     2581 ttatatcaat ttaagaaagt attatagtga aacattgggt caaagtgcac tatcaataat
     2641 acatcttgaa aatggagaga agacggaatt tttaaatact agaacaccaa taaaagatat
     2701 accaatatat cttcgtttgt atatacaagg tcgtaagtca tattttgaat ggagttatga
     2761 tgaaaaaaac taccaaagaa taggaaaggt atttgataca actaagtttt cagatgagta
     2821 ctgtaaatat ggagaattta caggtacgtt tataggatta acttgtgcag atcgtgtaaa
     2881 gcataaacac tatgctgatt ttgatttttt tgaatatata gcagatgaat caaaagatgt
     2941 cgattaataa aatcattact aaaattttca gagcaaaggt aagtcttatg ctctgaaata
     3001 agaaacatta gaaggtaaat cttacaaaaa ggagtatggt attatgaaca cagaagttaa
     3061 tgttaaagca actactatta gatatgaaaa gatttcattt tttgaaagaa tttcttatgg
     3121 ttgtggggat ttgggatgca atatcatata ctcagctatg tcagcatttt tgttgtttta
     3181 ttacactaat tatgcagagg ttagtgcagc agctgtaggt agtattatgc ttgtatctag
     3241 gatactggat gggtttagtg acttaacaat gggaattata gtagatagaa ctaaatccaa
     3301 atacggtaaa gctaggcctt ggattttgag aatggcgatt ccttttgcta tagcagcaat
     3361 attattattt tctgttccat caaatcttgg tatcacttct aaattaattt atatttttat
     3421 tacatataac ttagtttcta ctgttattta tacagcaatc aatgtaccct atgcaactct
     3481 aaattcactt ataactcaag accagtatga aagaggagta ttaagtatat ttagaatgat
     3541 attagccact tgtggtacct tactaataaa tggacttaca ctacctctag ttgaatactt
     3601 tggaaataat ttatcagcat ggactaaaac attctttgta tttggaatag catctataat
     3661 ggtatttttt ataacattta ctggaaccaa ggaaagagta aaagcagtta agcaagatag
     3721 gaatgaagta ataccattta aaataggtat taaatcttta tttagaaata agtactggat
     3781 acaaataaca ttatgtttag tttgcatatt tattgtattt gcgataaatg gtggttcatc
     3841 agtatattat gcaaagttta ttttaggaga tgaaaaatta tttggtccta taaatatggt
     3901 ttcaaatata tcccaaatta tagctatgtt tatggttgct ccttttataa aaaaatttgg
     3961 aaaaagaaat gtattaatag taggttcaat aatattaata tcgtcaaatg ttatgtttat
     4021 aatagttgga caaaactata ttggagtaat atttgcaagt gttataaagg gaataggaag
     4081 tgctggaata gcatcaacaa tgtttgctat tgtttcagat actattgaat atggagaatg
     4141 gaagactgga tatagaactg agggtcttat aaatagtgca tctagttttg ggtttaaagt
     4201 gggaaatggg ttaggttcag cgatattagg tgcagtatta tcaattggtg gttatgtagg
     4261 aacatcagct actcaaagtg atttagctat agtatcaata aaagtatgtt ttatatacct
     4321 acctatattt ataacagtat tacaaattat cataatgtct ttttataagc ttgataaaga
     4381 atatagtact atacttaatg aattaaatac taaataatga aaaagtttct tataaagtct
     4441 taagtttaat taaggtctat tgtaaatttt ctttataaaa aaactattat atatttataa
     4501 tatattggag aatttttaga gaagcgaata ataaaaatat aagaagacct caaatgatga
     4561 aactgttggt tttttagaga tgatttaata agttatcgtt atttaaagca ttataagtgg
     4621 acaattaatt ttgagtttat ggagatgata tttaacaatt aaaagtaata tataatatgc
     4681 attaagaagc aatttttcaa ctgtttatga agatggtggt acaagaaaag tatcaccttt
     4741 tttttgttat acaaaaaaac agttgacttt ttatatgaac cccttctata atgttactga
     4801 aattaagtaa aatgattgaa caaatgtaaa gatgattaaa aagcttgatt ttggaggaaa
     4861 gcaaatgaaa aaaattggag acttaagatt aatgatgaag tgttgttcgc tttattacga
     4921 agataatttg aatcaacagg aaatagcaaa tcaattggga atatcaagac caacaatatc
     4981 aagaatatta aaagaagctt ttgaacaagg aatagtaaaa atacagatag ttgatgtttt
     5041 gaaaaatgat taccaaaaaa tagagagaag tttggagaga aaatataaat taaaagaagt
     5101 aatagtagta gatgataaac aggatgcact aacacaaaaa caagaactag caagagctgt
     5161 atctgaatat ttaactagag tagtaaagga aaatgatata ataggtgttt caataggaac
     5221 aacactaaaa gaaataccaa gatatgtgga aaaaagtaac tgtaaaaatg ttacatttat
     5281 acctctatta ggagggattg gagacaatga aatagatatt catgcaaatc aaatagcagt
     5341 aagtttggcg agggcttttg gaggtgattt taaattatta catgctccag ctgttatgtc
     5401 tgacttgtct acaaaagaga aactttgcaa agacgaaaaa attaaagaag tactagattt
     5461 aatagataaa acaactatag caattgtggg tataggaaat cctatgagtt taaactcaac
     5521 aataatggct tctggataca tgaatgaagc agatatagaa gatttaaaaa aatacaactc
     5581 aataggtgca atttgtttac aagcctttga taaagaagga aaaacttcta tacttgaatt
     5641 taatcaaaga gtattaggag tgaaactgga agatttaaaa aagataaaaa gaacaatagg
     5701 tgtcgcttca ggagatgaaa aaatagaggc aataaaggca tcattaaaag cgaaatttat
     5761 aaatagttta gcaattaatc acagtttagc acttaaatta ttagaagatt gtgactaata
     5821 gaaagataga taaaagttaa caggagggaa tgtatatgtc aatatcaaaa gaaacattgc
     5881 ttgaaatgta taagagaatg aatcaggcta gaaaatttga agagaaagtt agttggtttt
     5941 ttgcaagggg tatggttcat ggaacaactc acttatctgt aggtcaagaa gcgtcaagtg
     6001 tggcggctgt aatggcttta gaaaaaggag atttagtatc attaactcat agaggacata
     6061 gtcaatttat aggaatggga atagacctaa ataaaatgat ggctgaactt atgggtaaag
     6121 aaactggatt ttgtaaggga aaaggaggct caatgcatat agcagatatt gagtcaggta
     6181 acttaggagc aaatggagtt gtaggtggag gtcttacaat agctccagga gctgcactta
     6241 cacaacagta taaaaaaaca ggtaaaatag ttttatgtag ttttggtgat ggagcatcaa
     6301 atgaaggtac ttttcatgaa ggaataaatt tatcttcaat atggaaattg ccaataatat
     6361 tttactgtga gaataattta tacggtatgt caacaagcat aaaacgtcat atgaatatag
     6421 aaaatattgc cactagagca gcctcttatg gaatagaagg aatatcaata gatggatata
     6481 acccaataga agtatacgaa acagtacaaa aagctgctga gaagtgtaga agaggagaag
     6541 gacctgtatt aattgaaagt agaacttata gatggttagg tcattcaaaa tcagatgcaa
     6601 atgtatatag aacaaaagaa gaaatagaat catggaaagc aaaagaccct attgaatttt
     6661 taaaaaatta tcttatagaa aataatctat ctaatgaaga tgaattagat aagattcaag
     6721 aatttgcaaa gcaatcaata gaagatgcag tagagtttgc tcaaaatagc ccaaatccaa
     6781 aaatagagtc tttattagag gatgtatatg cagactaaga atggtaaagg aggaaaataa
     6841 aaatgagtac aagggaatta acatatgcac aagcaataaa ggaagcaatg tcagaagaaa
     6901 tgagaagaga tgaaaatgta atatttatgg gagaagatat aggaatatat ggaggagcat
     6961 ttggtgtatc agttggtatg atagatgaat ttggaccaga aagagtaagg gatactccta
     7021 tatcagaagc agctatagca ggagcggcag caggtgcagc agcaactggt ttaagaccta
     7081 ttatggaagt aatgtttatg gactttgtaa caatttctat ggatgcaatt gtaaatcaag
     7141 cggcaaaaat gagatatatg tttggaggaa aagcacaagt tccaatggtt gttagatgtc
     7201 caggtggttc tggaactggt tcagcagagc aacattcaca aagtttagaa gcttggtttt
     7261 gccatgtacc aggagtaaaa gtagttgcac cttctacacc agcagatgct aagggattgt
     7321 tgaaagcagc tatacgagat aataatccag tcatatttgt tgagaataaa ttgttataca
     7381 gaaaaaaagg tgttgtgcca gaagatgatt atgtaattga gattggaaag gcagatatta
     7441 aaagagaggg tactgatgtt acagtaatca cttatggaag aatgttacaa agtgttgaag
     7501 aagcagcaga gactctatca aaagaaaata taaatgttga aattatagat ttaagaactt
     7561 tatatccatt agataaagag actatagtga agagtgtatg taaaactgga agagttttaa
     7621 tttgccatga agcggctaag actggtgggc taggtggaga aatatcagcc ctgataacag
     7681 aaagtgaatc atttgattat ttagatgctc cagtaaaaag aatttgtgga aaagatgtgc
     7741 caataccata taatccagat ttagaaaaag cagttgtccc aagagttgat gaaatagaag
     7801 aagcaataaa atctttaatt gttagataag gagggataga tatggtggca aataaaataa
     7861 aagccacacc agcagctaga agtcaggcaa gaaaagacaa tataaaatta gataggctga
     7921 ttggaagtgg agaaaatgga aggattcatt tagtggatgt gttaaattat ttaaaagata
     7981 ataaagcaaa tacgactcct ttggctagaa gaatagcaga agatttaaat atagatttag
     8041 aaactattgt aggtactgga tataatggaa agataagaaa gtgtgatgtg gaaaaactaa
     8101 ctgcaaaaga aactattgta tcaactaata catctaaatc taatgagaaa aaagagatga
     8161 aaattgaaaa tgaaaattca agcatgttta acacagtaga aggaatattt gaaaagccaa
     8221 atccaatgag agctactgta gcaaaacgaa tgtcagaaag ttattttagt gctcctgtat
     8281 ttacattcaa tatagaagta gatgctacag aattgaaggc acttagagca aagttaatgg
     8341 acacagtaaa agaatcaact ggagtaaaat taactatgac agatttaata gtaatggcag
     8401 tatctaaaat acttccaaat catcaagcct taaactcagc atggacagac gagggtatat
     8461 ttagatataa agatgtaaat atagctattg cagttggttt agatgaaggt ttatatgttc
     8521 cggtagtaaa aaatgcaaac aaaaagtctt taaaagaaat agcaaaagaa agcaaagaac
     8581 ttgcggaaaa agtaaagaca gggaaactta tgccagcaga ccaagaaggt aatacattta
     8641 caataagtaa tgttggaatg tatggtataa ctacatttac acctataata aatatgcctt
     8701 caagtgcaat cttaggggta ggtgctacac aagataagtt tgttccagta aatggagagg
     8761 ctaaaataaa acctataatg aacttatcat taacatcaga ccatagagta atagatggaa
     8821 cagtagctgc aaaattctta aaagatttaa aagaattgtt agaaaaccct ttatcaatgc
     8881 ttgtataggt tttgatattt cgttactaat ataaatagtt tgaatagatt acattattat
     8941 aattgtttca gtaaatttaa agcttttata tattaagaat tatttttaac agtaactata
     9001 tgtgaatatg atttgcttaa ttaaaatata tgaaaataaa taggaggaga gcctatgtca
     9061 gtagaagtaa taatgccaaa agcaggagta gccatggaag aaggtactat agtatcttgg
     9121 ctaaagcaag aaggcgaaga agtaaagatt ggagaaccta tactagaaat aacaacagat
     9181 aaagttaata tggagataga gtcagaagga gaaggtacac ttgctgtaat catacataaa
     9241 gaggaagggg aagtattacc agtatttaca gtaataggtg taatagcaga aaaaggggaa
     9301 aatcaagagg aagtaaaagc taaatattca tctggaaata tttcaaaaga agatatagtt
     9361 gaagagaatc aaaatacaga agttaaagaa gaaaaaataa acaaaaaaga gtgtaatcat
     9421 gattatgatg tagtggtaat aggtggaggg cctgggggat atttatcagc attaaaagca
     9481 gctcttttag gtggcagagt tgctttagtt gaagaaaata tactaggagg tacttgctta
     9541 aatagaggtt gcatccctac aaagacttat ataaaaacag cagaaatatt agaagaaatt
     9601 gaccagttat caaaaagagg cgtaaaagta actgtagata aagaacagga cattaagaag
     9661 gccataaaat ataaaaatag agtagttaaa aaacttacag ctggtgttgg cggactatta
     9721 aaaagtagag atgtagaggt gtttaatctt aaagctagtg ttaaagaaga gcataaagta
     9781 attttatcag atggaaaagt tttagataca gaaaatataa taattgctac aggttccaaa
     9841 gtaaggatat tacctataaa aggaatagaa tcaaatttaa taataactag tacagaagcg
     9901 ttagatttag aaacagtgcc agaagaatta gtaataattg gtggtggagt gattggttgt
     9961 gaatttgcag agatattcaa ttcaagaggt tctaaagtaa ctatagtaga aatggaagat
    10021 agagtaatcc caagaatgga taaagagtta agcgaatctt taaaatattc ttttagtaaa
    10081 aaaggtataa atgtattaac taaaaagaaa gtttctgaat ttaaagaaga aggaaataac
    10141 atattagtgt gcattgaagg tgaagagcca ataaaagcag atttatgtct atatgctata
    10201 ggaagagaag ctaatctttc tggcatagag gacttagata ttaaaataga taaaggttca
    10261 atagttgtaa atagtaaaat ggaaacaagt ataccaagca tatatgcagt aggtgatgtt
    10321 actggaggtg taatgttagc tcatgctgct tttaaaatgg gagaggtagc agcttcaaat
    10381 gctctaggta tgaataaaga agttgactta ggtgcattac ctagttgtgt ttatacaatt
    10441 ccagaagtag catcagttgg tattacagaa gaggatgcta gaaaaaaata taatgtaaaa
    10501 gttggtaagt ttaattttgc tggaaatgga agagctttag cttctggcca agaacaagga
    10561 tatgtgaaag tagtagcaga tgctaaatat ggagaaattt taggtataca tatgtttgga
    10621 tgtggtgtag cagaacttat aaatcatgca gcagcattta aagcattaga gatacctact
    10681 gatgaagcaa gtgagttgat atttggtcat ccatgtacct cagaagcttt aatggaagct
    10741 ttggcagatg taaatggaga atgtttacac ttacctaaaa aataaatcta aacataaatt
    10801 gttaatttaa gtttattatt aatattaatt tatatcataa agttttaaaa cacttactta
    10861 gccatacccc ttattatgca aataatttat ttgcataata aggggtattt tttatggaca
    10921 aatatagaat atagaatcct tgtagttttt gttgtacaaa aaagatagta atataaaata
    10981 acattatcat tttagaatga tttcaattgt aattaggaaa agtgattttt ttgtttttaa
    11041 aaaaagtatt atttgtaaaa tataaaaaaa aatttcttaa tatttgaata aaagaatagc
    11101 ttgatttaaa tgttattact ttataaaaaa ataataagta caaaaaaaac aatatatggc
    11161 atataagttg ctatataaat aataaggagg gaacaattat aaatgaggca aagaataaaa
    11221 aacttaattg aaaatgaaga taagaaaaat cctttaacag atgaagcgat ttcaagtagg
    11281 ttaaatattc gaagagaaga tgttaccttt ttgagaaatg agttaaaaat agaagattca
    11341 agacaaagaa gaaaagttgt tttaattaaa gctataaaag atattttaaa agaagaaaaa
    11401 actataaata aaaatgatat aacaagaaga ttaaacagca ttgggtttac agtatcaaga
    11461 tttacagtca tacaatactt aaaagagatt caagattctg gtgacctaaa acttgataaa
    11521 gagagtgatg ataaaaaaga agaaataatc aataatagag aaaatacaga tatagcattt
    11581 gataaactca taggtagtag tggtagctta agtacaatta taaaactagc caaagcagca
    11641 attctttatc cacctcatgg tcttcatact attattatgg gaccaactgg tgttggaaag
    11701 agtgagttag cagagtgtat gtataaattt gcagtagaat catccagatt ccctaagaat
    11761 gcaccattta tagtttttaa tgcagcagat tattcagaaa atccacagct tttgttatca
    11821 cagttatttg gacatgttaa aggtgctttt acaggagctg atgagaacaa agaaggacta
    11881 gtatcaaagg cggatggagg aatattgttt atagatgaaa tacatagatt acctcatgaa
    11941 ggtcaagaaa tactatttca actgattgat aaaggtatgg tcagaaagtt aggagaaaca
    12001 agactaacac ataaaataga tgtaatgatt atttcagcaa cttctgagcc aatagattct
    12061 catttactta atacatttaa aagacgtata cctgtaacaa ttgaaatacc agaattagtt
    12121 gcaagaccat taaatgagag gtttgacatt ataaataatt tcttcttaat agaagctcaa
    12181 agaatgggag caaacataca tataaagtca gatgtattaa aagctcttat gctttacgat
    12241 tgtataggta atgtaggtca acttagaagt gatattcaag tagcttgtgc taggggacta
    12301 ttaaaccaac ttacaaacaa acaaaaagaa gtaaatataa gcatatcaga tgtacctgga
    12361 tatgtaaaac aaggtcttat aaaaatcaga aattgtagag gtaaaataga aaattatgta
    12421 gatggggatt taattataga ttcaaatctt caaggtgaga tagaaaagaa aaatgaagat
    12481 atatatactt ttccagatga aatatacaaa cttactgaaa gaagacatat tgatttattg
    12541 gaacaaggtt tagattttga tgttataaat agaatcattg gaggagaatt agaaagttgt
    12601 attcaaaaat acataaaaca agttaaaaaa gtttctatta gtaacaatct tccagatata
    12661 agtccgattg ttggaaaata tatcgtagag ctaacttatg aagtaatcaa aatagcatca
    12721 caatacctca gttcactaga gccgaatcta catttatgtt tagcagttca cctaaaagca
    12781 gcttttgaga gattaaaaga aggaaaagta atatcaaatg ctcatttgaa agaaataata
    12841 ggaaattatc cgctagaata ttctgtagca gaaaaagtag ctcaatattt aaaagaaaga
    12901 tataacataa acttaccaaa agaagaaata ggtataatag caatatattt taaaatgagt
    12961 agtagaagag atatgtctga acaaaaaaag ataggtgtac ttcttatggc tcacggaaga
    13021 gtagcatctt ctgtatgtga ggttacaaat aaactattag gaattaatca cgcaaggtat
    13081 ttagatatgc catttgagaa gaaaccggaa gaaatgattg aagaagctac tacaataata
    13141 aaagatattg atgaaggtag aggtgtgatt atattagtag atatgggttc actttcatat
    13201 tttggagata ttatttctgg aaagactgga attaatataa ggacaatatc tagatttgat
    13261 acattgcttt ctatagaagt tatacgaaga gcagttttac cagatttaac aatagacgag
    13321 atagttgatg aagttaaagg tatagagcca caagtaaaag aaaaggttag taaatataac
    13381 aatacaaaag tagattttag aaaacctata attttaacat tgtgtattac tggattaggg
    13441 ggagcttgta agataaaaaa aatcctagag gaaaatatta aaagtataac tgaaaaggtt
    13501 gatatagaac ctataggtat gattgaatat ccaagtcttg atttagagat aaaacaaata
    13561 agcaatgata aaaatgtaat cgccatagtt ggaacaattg accctcagat agcaaatatc
    13621 ccttttttta gtctagtaga cataaatgat aaagcaaagt taaatcaaat aaataacctg
    13681 attgatacaa atcaaattgg taaaacaaat aaaaaatatg aattatcaga tttaatacat
    13741 aaagaagata tatttgtgga tttacaggtt aaatcaaaag aagaagttct aaagtatatg
    13801 agtaaaaaac ttagagagaa aggttatgtg acaaaaaatt tctataaaaa agtagtggaa
    13861 agagataact tatttccaac tgaattatca ggtgaaatag caatacctca cacagatgga
    13921 atagatgtaa ttaggccagc tataagtata gccatcttaa agaatcctat tgtgtggagt
    13981 aaaagtaaag taaaaataat acttttatta gctgtagacc aaaagtgttt taatccatta
    14041 agcactctac tttcatttat agaaacagat gaatttaaga agataaaaaa cattaaagat
    14101 agagatttta ttagggagat gatattagat ggagtcaatg aaaataatta gtagtaattt
    14161 aatttttaaa aatattgaag tgtctaacaa tgaagatgca ttaaagtttt tgggacaacg
    14221 tttgtttgac gaacagtatg taaaagaaag ctacatacaa gctgttgtag caagagaaaa
    14281 aaagtatgct acaggattac caacagaaat atacggagtg gcaataccac atacagatat
    14341 agtacatgta aatgagccag ggatagcaat tggaatactg aataaacctg taaaatttat
    14401 aatgatgggt acagacgata ctgaaattga tgtaaaagtt gtatttatgt tagcagtgaa
    14461 agagccacaa gaacaattgc aactattaga aaaacttatg actatatttc aagataagaa
    14521 tatactaaat aatattgttg acttaagtga agaaagtgtg agtgatttat taaactctaa
    14581 attgaaaaat taaacatgta aagtatgtta aaaaatataa aaagattatt aaagatttct
    14641 aactataggt atctttaaaa taaaataatt ttaatggggg tgactaatgt gactaaaaag
    14701 atattagttg catgtggaac aggagtatgt acttcaacaa ttgctataaa caaattaaag
    14761 aaagcatttc aagacattgg taaattggat atggttaata ttacacaatg taaagtagta
    14821 gaagttgcat caaaagcacc tgattatgat ttaatagttt gtactacaca agtatctagt
    14881 agtataaaaa caccagtgat aaatggatta ccttttttaa ctggtgtcgg aatggataaa
    14941 ttaatcaatg atgtattaga agagttagaa ttataattaa aaaaattaag gaacatggtc
    15001 tctaaaatta taagttttag agacttaaaa ttaaataatg gagggtcttt atatggagat
    15061 attaaatttc attgttggtc taggcgcaca agtaatgatg ccaataataa tatgtatttt
    15121 tggtttgata ttaggtacaa aactaggaaa atctttaaga gctggtttaa ctgtgggagt
    15181 tgggtttata ggtcttaata ctattatagc tttgctaaca gataatttag gtccagctac
    15241 acaacaaatg gttaagaatt tagggttaag tttatcaata atagatgtag gatggccagc
    15301 agcatcagcc atagcttttg catcaacagt aggggcttta attattccaa taggtcttgt
    15361 agtaaatata gttatgctaa ttactaatac cactcaaact gttgatgtag atatatggga
    15421 ttactggcat tttgctttta caggagcttt agtggcaggg gctactcaaa gtgtaatgtg
    15481 gggagtattt gcagcagtag caaatatggt aattgtattg gtaatggcag atttaactgc
    15541 tccaggtata gaagaatatt taggtatgcc aggtatatct cttcctcatg gatttacaca
    15601 ggcatttgtt ccaattgcta tagtagtaaa taaattatta gacctaatac caggtataaa
    15661 taagattgag ataaatgctg atacattgca gaaaaaattt ggattatttg gagaaccttt
    15721 aataatgggg tctgtaatag gtgtaataat tggtattgcc gcgaaatatg atataaaagg
    15781 aatattacaa ttaggtgtaa ctatgggagc tgtattaata ttaattccaa agatggcagc
    15841 attattaatg gaaggattat taccagtatc agaagctgca caggaattta ttgagaagag
    15901 atttaaaaac agaggtaaga tttatatagg cttagattca gcagtaggaa taggtcatcc
    15961 tgttacatta tcagttgcac tagtgttggt tcctttgact atacttatag cagctatatt
    16021 gccaggaaat aaagttttac catttgcaga tttagcagta attccatttg cacttgtatt
    16081 aattgtacca attacaaagg gaaatgtgtt tagaacatta ataataggaa taattataat
    16141 tacttctgga ttattaatcg caactaactt agctcctttg tttacccaaa tggctttaaa
    16201 tgctagtttt aaaatgcctg aaggagcaac tatgatttca agtatttgtg atggtgcgaa
    16261 tccattaagt tgggtatttg taaaagttat gaactataaa gttattggag gagtagtttt
    16321 tggagttata gctttaggaa tggctattta caataggaat agaataataa atgaaaataa
    16381 aaagctatct atagaagaat aaggttaaaa caattttatt aaggagtgta taggttatga
    16441 aaaaatataa atatgattct
//
LOCUS       DAEMAE010000077        16220 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEMAE010000077 DAEMAE010000000
VERSION     DAEMAE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.079"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            184..3087
                     /locus_tag="KPH93_001914"
     CDS             184..3087
                     /locus_tag="KPH93_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688734.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRILMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGNLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEDIFSMSDLNSFVSDSLVKLVKKLMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNINTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMIDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLDILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3120..3566
                     /locus_tag="KPH93_001915"
     CDS             3120..3566
                     /locus_tag="KPH93_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688735.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSVMCKKEYVKDTYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3689..3991
                     /locus_tag="KPH93_001916"
     CDS             3689..3991
                     /locus_tag="KPH93_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688736.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEETFFEEVLTVIEGIDK"
     gene            4172..5578
                     /locus_tag="KPH93_001917"
     CDS             4172..5578
                     /locus_tag="KPH93_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG1688737.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLAVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5922..6980
                     /locus_tag="KPH93_001918"
     CDS             5922..6980
                     /locus_tag="KPH93_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1688738.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPSTIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFAIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIRKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGAIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7082..7768
                     /gene="araD"
                     /locus_tag="KPH93_001919"
     CDS             7082..7768
                     /gene="araD"
                     /locus_tag="KPH93_001919"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG1688739.1"
                     /translation="MLENLKEEVLNANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEEVGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7786..8433
                     /locus_tag="KPH93_001920"
     CDS             7786..8433
                     /locus_tag="KPH93_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG1688740.1"
                     /translation="MLESLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLNEALLKSRYVEEVAEIYYKSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8606..9973)
                     /locus_tag="KPH93_001921"
     CDS             complement(8606..9973)
                     /locus_tag="KPH93_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG1688741.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRAKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTISLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10162..10250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10678..12798
                     /locus_tag="KPH93_001922"
     CDS             10678..12798
                     /locus_tag="KPH93_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG1688742.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKYLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSRICNKLLKSNITANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDILKVKGLKITSGKTNPPPYLNEGTLLTAMEKNSLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12902..14155
                     /locus_tag="KPH93_001923"
     CDS             12902..14155
                     /locus_tag="KPH93_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG1688743.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLMNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDSDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTSIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMAKGGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICSE"
     gene            complement(14309..15370)
                     /gene="ytvI"
                     /locus_tag="KPH93_001924"
     CDS             complement(14309..15370)
                     /gene="ytvI"
                     /locus_tag="KPH93_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG1688744.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLVFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISIIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15644..>16220
                     /locus_tag="KPH93_001925"
     CDS             15644..>16220
                     /locus_tag="KPH93_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG1688745.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDI"
BASE COUNT         6464 a         1812 c         2804 g         5140 t
ORIGIN      
        1 atttaagatt atgaataact aatacattta gtaaattact aaatatacaa aatgtaaata
       61 cttatcaaaa tgtataaata cttatgacat ctagttactt acagggtttg agtagaaaaa
      121 aatatcaaag taagtatggc atgatagttg cttatatatt tatcaagaaa ggagttatta
      181 agcatggagt tgagagataa acttgtaaac atcataaata cagaaaataa aaaagatcca
      241 ttaacagatg ttcaaatagc aaagttttta agtacaacta gagagaacat aactaatttg
      301 agaaaagaac ttaatataag taattcaagg caaagaagat atccttattt aaaatcagca
      361 atttcggcaa tattacaaaa gaataagaat atttctatat cagaaattac aagggaattg
      421 atgacagaag gctttaatat atctagaaga gttgtggagg aacttttacc taaagaaagt
      481 ttagaaatgg atgtagtaga ggaatctgaa gaagaatcga atgacccatt tgaaactctg
      541 ataggtaata aagggagtct tagaaatgct gtggagcagg ctaaatctgc tatattgtat
      601 ccacccaaag ggttaccaac gttgattatt ggggaaagtg gagttggtaa gagtttattt
      661 tctagacata tgtatgagtt tgcaaagcaa aaaaagatag taaaagaaag ctccaatttt
      721 gttgtattta actgtgctga ttatagtgac aatccacaat tattattatc tttgttattt
      781 ggatataaaa aaggtgcatt tacaggtgct gaatatgata caccaggtct tgtagaagaa
      841 gctgatgaag gagttttatt tttggatgaa atacatagat tgcctccaaa aggtcaagaa
      901 atactatttt ctatcttaga taggggaaag tttaggcgat taggtgaaac taatgctgaa
      961 agaaaagtaa gtattatgtt gattggggca actactgaaa atgtagagac taatttatta
     1021 ttaacattca gaagaaggat actaatgcta ataactttgc caccattaca tgatagatta
     1081 ctaaaagaga agatagacct tatatacaat atctttcaac aagagtgtaa tagaataaac
     1141 gcaaaaatat ttgtagataa aaatgtgatt gaaatacttg cactaaaaaa gtttagtgga
     1201 aatataggac aattacaaaa tatgattcag gttctttgtg cgagagcttt tatgaagttt
     1261 ataaattcca aagatgaaga tagtgaatcg atagtagtag tagatataaa tgaagtttta
     1321 aagcttaaag attcttttaa agatgctgcc tttcaagaaa ttgaatatac agagataaga
     1381 aaatatttga aaaatgctat atttatacca tttaatttag aagaatcatc agtaaaagga
     1441 aatcttttaa gtgatgaata tatattacca gaggacatat acaaaacaat tgaaaagaaa
     1501 tattatgacc taaaatccct agacattgaa gatatagata tagagaatat tttatggact
     1561 tttattttaa atagatttgc tgacttaaag tttaatctcg atagtaatga agatattttt
     1621 tcaatgagtg atttaaatag ttttgtaagt gatagtttag tcaaactggt aaaaaaactt
     1681 atgaatgata tagttgaaaa aaatatgaat ttagaagtaa ataaaaatat gtttaaatat
     1741 ctagcaatac acttagaaga agctataaaa agaattaagc taaatcaaaa gataataaat
     1801 gtaaatttag aaaaaataaa gattgacttt tcaaaagagt atgaaattag taaaagtttt
     1861 gcaataaaaa ttgaggaatc aattggtatt aagattccag atgatgaaat tggatttata
     1921 acgctctaca taaagtctgc tctaaaaaat gaagtgaaaa aagataaagt agggttaata
     1981 ataatttctc atggacgtat agctacagag actgtaaatg tagttaaaga acttttaggt
     2041 gtaaagtttc ctgtggctat agatatgcct ttagatgaaa aaccaataaa tatatacaat
     2101 aaggcagtag aattgtcaaa aataatagac caagggaaag gcatattatt tttggtggat
     2161 attggttcac ttacaaacat aggtcaaata gtaaataaga gaacgaatat aaacactaaa
     2221 actattgata gagtagacct cttaatggca ctagaagcaa ctagaaaagt ttctatggga
     2281 gaggaagaac ttgatgaaat atttttttct ttgctaaaag ataggatggg atataactat
     2341 gaattagaaa aacacataga taaatcaaat gcaattgtaa cattgtgtct tactggagaa
     2401 ggaactgcta agtatataag taaaacattg gaagaaaagt atgataacac taaatgctat
     2461 caaatgagtg cattggatga aaatctattt tgtaaaattg aaaatctaaa ggagacaaac
     2521 aacatattag ctattgttgg gacaataaat cctaagatac caggtataaa ttttataccc
     2581 tataataaag aacttttcaa aaatttggat atctatctat caaaatcaaa acaacaagat
     2641 gacagaccag ttaaatcgta tgaaagaatg attgatgagg atttagtgat ttttgaacca
     2701 gatatatatt ttaaaaagga tttattagaa tatgtatgtt caattcttat aaataaagaa
     2761 tatgtagaaa aagattactt agattctgtt ttacatagag aagaaatgct tccaacatat
     2821 tctaaaggag ctataggtgt gcctcatgga gattcttcta ctgtaaatag tacaaggttt
     2881 gtttttgtta aactaaagaa tcctattgat tggggtgtag gtaatgtaaa tttcatattt
     2941 atgccagttt ttcaggctaa tgataaagag attgttaaaa atgttcttga tatactaaaa
     3001 gatgcagaat ttatgaacaa tgccaataag tgttttgata aagatgcttt tagaaggatt
     3061 atatttgata aatttaaaca tctgtagcca tagcaaaaaa attgagagga gttagagtaa
     3121 tgaatgtaga tttaatatca ttaggtttag aatctaatga ttatgaaagt gttattgaag
     3181 aattaggaag tgtaatgtgt aaaaaagaat atgtaaaaga cacatacata aatgctgtct
     3241 tggaaagaga aaggacatta ccaacaggtc tagatattgg agaaatgtgt gttgctattc
     3301 cacatacaga ttcaaagcat gtaaatgagt ctaatgtagc tgtagggata ttgaaaaatc
     3361 ctgtaaagtt taattcaatg atagacccaa aagatagact tgatgtagaa cttgtatttt
     3421 tattagcagt aaaaaaccca gattcacaag taaaactgtt aaaagattta atgtcagttt
     3481 ttcaaaatat aaagttatta aaaaatataa aaaacgcatc aacaaaagaa gaagtagcta
     3541 aattattgga ctttatagaa atttaatgta ggagaatttt ataaataaaa aattaaaatt
     3601 taaacaatca aagtaaatat tattttttta ataggtttaa tcttatataa aataaaaatt
     3661 tagagtgaaa aattaggagg aaattattat gaaacaattt aatgtattat cagtttgtgg
     3721 ttcagggaca gttacatcat ctatggtggc aggtaaatta aaagaagtat taggtaataa
     3781 ggggatttct attactacaa cagaagctag accaacagaa gcactaaatt tagcacaatc
     3841 aggaagattt gactttataa catttacgag tccacttcaa acaggcgatt atggaatacc
     3901 aactataaat gcatttgctt gcctaactgg tataggggaa gagacattct ttgaagaagt
     3961 tttgactgtt attgaaggga tagataagta agttttggag atgtgaattt atagtattta
     4021 aatcaatgat ttagatacta taataagcta atattaacta aatatttgtg gaaaaaacat
     4081 tttatatgtt gtcataaatt aaaaaattat aaaaatagtc acttaagtat taaattaagc
     4141 ttataaatat ttataagtag gagggaaaaa gatgttagaa tttatgaaat ctataatcga
     4201 tacatttggt tctgcaatta tagttccaat tataatattc ataatagcaa agatatttaa
     4261 agtaactact aagaaatcgt ttctttctgc tgtgtatgca ggagttgctt tacaaggttt
     4321 tacactgatt ttaaattcat ttacaccaat aataacacca gttattaata ggatggttga
     4381 gagtacagga gtaaacctac cagtttttga tgttggttgg caagcaacat cattggttgc
     4441 attttcaaca agtgcaggta tgatatattt aggtcttggt atattattac aaacaatttt
     4501 atttttgata aaatggacag acgtgtttca accatcagat ttatggaata actattctta
     4561 tatggtatgg ggagcaatgg ttataggagt aacaggaaat tttcctttag gtatagcatg
     4621 tatggtttta ttaaacttat atagtttatt aatatcagag ttagttgcaa agagatggtc
     4681 cagttactat agatatccta attgtacgat aatagctatg cataatgtcg aggcttctgt
     4741 gtttgcagta tttgcagacc ctatatacaa taaattggga ttaaataaga taaaattaaa
     4801 tccaaaagaa ttagagaaga aattagggtt tttaggtgaa ccaattactt taggtctctt
     4861 tttaggcatg tttataggta tattaggcaa tatgacaaga ataaatacta tggaagcttg
     4921 gggagaaata atgaaagtag gtatatctac aagtgcagtt atggcaatct tccctaaagt
     4981 agcaagcatg tttgctcaag catttgcacc tataacagaa gcagctagaa agattatgca
     5041 aaaagctgga aaccgtgagt ggtacatagc agtaaatgat gctgttggat atggtgaacc
     5101 tgcaacttta atatcaggtc ttatattaat tccaataatg cttgtaatag ctatggtact
     5161 tcctggtaac aaggtattac cagttgtgga tttattggca atcccatata tggttcaagg
     5221 tttggttgcc atacataatg gtaatatacc aaaagtatta gtttcaggta taatttggtt
     5281 tggattagga ttatatgttt gtacatctac agcaccatta tttacagata tggctacaaa
     5341 tataggtgta gctatacctg caggagcaat gttaataaca agttttaata tactaggaaa
     5401 acctttgatg ggattagttt tctttgcatt tctaagtgca aatccaattt atataggtct
     5461 tgcagttgta atttactttg ttctatgggc attgtttaga aaaaacaaaa caagtattct
     5521 tgattattta gaaaaacaag cacttaaaaa tgtagaagaa gagcctgtag cagtttaagt
     5581 ttataaaaat gaaataagaa taattaaagg agtttataat tgattttata taagtatcag
     5641 ataatacctg agaaatattt aaatgaaata tagacttctt tttgtttgat aatatttgta
     5701 attataatac attgtatttt tatagttgag ttttctgtat aacctgccat ttaaaacata
     5761 ctcttttata tttatacagt aatgtggttt aattttataa aatgtaatcc ctactggtaa
     5821 aagactttgt atataaggca tttgattgaa taaaccattt atcttttgta tggcatagat
     5881 attgctatat agaagtatat agcatgaagg gagatattta tatgggaaat aaaatgagag
     5941 catcagtatt gtataatgta ggtgatgtca gatatgaaat ggtagatata ccagaaataa
     6001 cagatacaca agtgcttgta aatgtaaaat atgttgggat atgtggttca gatttaccta
     6061 ggtctatggt aagtggtttg agtggaaata caaaatatcc attgatttta gggcatgaat
     6121 tttctggtga agttgttaaa attggagaaa aagtaaagca tattaatgta ggagatagag
     6181 tagctgttgc accattagta ccttgtggaa aatgtgatta ttgtaatgaa ggtaattttg
     6241 gcttgtgtga tgattacaat ataattggaa caagagtaaa tggagcgttt gcagaatatg
     6301 taagagttcc agaagaacat attttaaaac taccagatac attggattat gaaactgctg
     6361 ccggaattga accatcaact atagcttatc atgggatttc aaagtctaat attagagtag
     6421 gagatagtgt agttgtgtta ggatgtggtc caataggtca gtttgctata caatgggcaa
     6481 aggtgtttgg tgcatctaag attatagcag ttgatatttt tgatgaaaaa ttagaacttt
     6541 ctaaacttct aggagcaaac tatatactta actcaaaaga agtaaatgta atcaaagaaa
     6601 taagaaaaat aacaaatggt ggtgctgatg ttgtaataga gactgctggt agccgattta
     6661 cacaagaaca atctctattt gtagcaaaaa agagaggaaa tatagtgttt gtaggtatat
     6721 cacacacaga attaccactt agtgcagacg ctactgagtg tatattacgt ggagaactta
     6781 ccttgaaagg ttcttggaat tcatatacaa gcccttatcc tggaagagca tggactgcaa
     6841 cattggattt tatgggaaaa ggtgccatta tttttaaacc catgatttca cataaaatag
     6901 gtcttaatga agttggtgac tttttaagta aaatgagcaa gagagaaatt aatttcaata
     6961 aaatcctagt agaaatataa gaagaattta ataaaaaatt ctaaaagcaa ttaaatttta
     7021 taactatgac attaaataaa ataaaaataa ttttcttaaa taaaatatag gaggaagaaa
     7081 agtgttagaa aatttaaaag aagaagtatt aaatgcaaat ttagaattac ctaagaaaaa
     7141 gctaattact tatacttggg gaaatgtaag tgggatagat agagaaaaag gattagtggt
     7201 gataaaacca agtggtgtag agtatagtaa tatgactgta gaagatatgg ttgtagttga
     7261 tttagaggga aatgtagttg atggcaaatt aagaccttct tcagatactc cgactcatct
     7321 agttttatat aaagagtttg aagaagtggg gggaatagta catacacatt caagttgggc
     7381 aacaatttgg gctcaaatag gaaagtctat accatcttgt ggtactactc atgcagatta
     7441 tttttatgga agtataccat gtaccagaaa aatgactaga gaagaaattt gcaatgaata
     7501 cgaaaaggaa actggaaatg taataattga agaatttatg ggaagaaatc caattcattg
     7561 tccaggaata attgtaaatg accacggacc ttttacatgg ggaaaagatg caaatgaggc
     7621 tgtacacaat gcagtggttt tagaagaagt tgcaaagatg gcttattata cagagttaat
     7681 gagtcctgat aatattatgg ataaggttct tatgaataag catttttcaa gaaaacatgg
     7741 aaaaaatgca tactatggtc aaaaatgatt atctggaggt gaattatgct agagtcttta
     7801 aaaggtaaag ttgtggaaat agcaattaaa gcagagcatt atggtttatg taaagaaaag
     7861 actggtaatt ttagtattag agataaggat actggatata tacttataac tccatcaggt
     7921 ataggaagag aagaattaaa agttgaacat atttgtgtat tggatttgaa tggaaatgta
     7981 atagaagttg agaaaggtat taagccaagt agtgaggtat tgatgcatat tgaaatatac
     8041 aaaacaagag aaaatataaa tgctatatca catactcatt ctttatatgc aacagcattt
     8101 tcagtagcaa agaaaccaat tataccaatt gtatatgaat cagttaatta tggcggttat
     8161 gtagatattg ctccatacga aagaccagga actttaaaat tagctaagag tgtttcaaaa
     8221 ttattgatag atacagatgc atgcttatta gaaagacatg ggttgacaac tgtaggtaat
     8281 gatttaaacg aagcattatt aaaatcaaga tatgtggaag aggtagcaga aatatactat
     8341 aagtctttag tattaaatca atttaaagaa cttgaagtag tagatgttga tgagtttaag
     8401 tcttggaaat acccagaaga aataaagttt taatctaaat aattttcatt gaagtggtta
     8461 attaaaatat atgtattatt tatatcaatt actatattgt attatgttaa ttacggtatt
     8521 ttgtaaatat atttattaca ttttttcaaa aaaatataga atccgaaagc catataaaat
     8581 tgacttggga ttctatactt tttatttatg catttttctt tatctgattt tccggagaat
     8641 ttatatgttc aaaatatttt ttagtttcag atactattat aggtgataat gcaagtaaag
     8701 ctataaggtt aggtattgcc ataagtccat ttacaatatc agcaattatc caaacaactt
     8761 ctaactttaa gaaagcacct aataacacca tagccacaaa tacagttcta tacccattca
     8821 tgccttttac accaaacaga aattcaaaac atctttctcc ataataactc catcctaaaa
     8881 ttgtagtaaa tgcaaatagt gttaaactaa ttgtaagtaa tattggtcca aatgtaggaa
     8941 ttgctccgtt aaaagctgct tgtgtcataa cggctccatt taggtctgaa caccatactc
     9001 ctgaaataac aagagagaat ccagtaagag tacagattat aatagtatct ataaaagtac
     9061 ctgtcataga tataagacct tgttccgctg gccattcagt tttagcagca gctgcagcta
     9121 ttggagcact acctagacca gactcatttg aaaatacacc acgagcgata ccatttctaa
     9181 ttgccagtga catagttgct cctaaaaaac caccagtagc agcagtaggt gtaaatgcac
     9241 ttttaattat taacataaaa gtttcaggga ttttattcca aaaagatatt aaaatgattc
     9301 cacagattac aacatatata actgccataa aaggtactat tttagtagct acattagata
     9361 tacttttaag tcctccaaat acaaccaaag ctacaaatac tgctaataca acccctgtaa
     9421 ttctagggtc tatcccgaaa ctattattta ttgcatctgt tattgaattt acttgagtaa
     9481 aagtaccaat tcctaaaagt gcaactagta taccacttat agcgaaaaat attgcaagag
     9541 gtctccattt ttctcccata ccatttacta tgtaatacat tggcccacca gacacttgac
     9601 catttttatc tttagctcta tattttattg caagaacacc ttctgaatac ttagtagcca
     9661 ttccaaagaa tgctgctatc cacatccaaa aaagtgcacc aggtccacca gctttgatag
     9721 ctgtagcaac accaacaata ttaccagtac caactgtagc agctagagca gtacataagg
     9781 ctgcaaaact tgaaacatca ccactacctt tgttttcagc agagaatatt aacttaagag
     9841 ctcttggcag tttagttatt tgaacaactc caagcttaac agtaagtagt ataccagtac
     9901 caactaatag tactagtagg ggtggtcccc aaataaaagc atcaactttg tttaaaatat
     9961 caattagatt cataaaaaca ctccctcatt tttttgcttt acataatcaa tagaattttt
    10021 aatgaagtaa ttgacaggaa ataagtataa tttattaata atttctagtg taataaaaaa
    10081 agctaaatct aaaaaagatt cagctctgga tgtacactaa ttagcaacca aaataaaagt
    10141 attagtaaat actgtttttg gtagataatc cctgtccttt tgcctgagag attgagcata
    10201 aaagccttgc cccttcggcg cccataatgg gtctctccag agttccgtaa attttacagt
    10261 cctactttta aaagtacctg agagtgttac tccttcggtg tgctatatag caactcttct
    10321 ataaaatctt aaccggatat tgatttgtca tagcttattg tattaataat attaatataa
    10381 gtttttatgt tttgtcaact aaaaaattaa aaaacaaaga aaaaatataa aaatgtaagc
    10441 ctatttacga attataaact tagagagatg agtaatatac gtgaaaatga attttgtaat
    10501 acacatacaa atgaagtgta aaataagttt taaataaaaa aataataaaa tcttaaataa
    10561 ttaattagat atgattaaat tatggtaata ttatagttac gatattaaga aaagttctat
    10621 ttagttagat tttaagcgta atatgagatt atgtgtagag aaaggatgta tttttagatg
    10681 ggaaaaatat tagtgctagc agaaaaacct tctgttggaa gagatatagc aaaagtttta
    10741 gggtcaaaaa atgaaaaaaa tggttatata gaggggccaa agtatgtgat aacttgggca
    10801 ctaggtcatt tggttacact tgctgacccc gaaagttatg gagaaagata taaatcatgg
    10861 agcttagaag acctgccaat acttcccaag taccttaaga ctgttgtaat aaaaaagagt
    10921 ggaaagcagt ttaatacagt taaatcacaa atgaatagaa atgatataga tgaaatagtt
    10981 atagcaacag atgcaggtcg tgaaggagaa cttgtagcaa ggtggataat agaaaaatca
    11041 caggtaaaaa aacctataaa acgtctttgg atttcttcaa gtacagacaa agcaataaaa
    11101 gacggatttg caaaattaag aagtggaaaa gaatatgaaa atttgtatta ttcagcaata
    11161 gcacgagcag aagctgattg gattattgga ataaatgcaa ccagagcttt gacaacaaag
    11221 tacaatgcac agctttcttg tggaagagta caaactccta ctcttgcaat gttgcttaaa
    11281 agagaagaag aaataagaaa tttcaaacct aaagagtatt atggattaga attaattgct
    11341 acaaagggaa attcagatat aaaatttatt tggaatgata aaaacaataa ttcttcaact
    11401 tttagtaaag aaaagataga atccacatta aaaaaagtaa aaggtgtaga cataaaaata
    11461 tctgaagtaa acaagtcata taagaaaaaa tattcacctg cactctatga tttaacagaa
    11521 ttacaaagag atgcaaataa aatgtttgga ttttcagcaa aagaaacttt atctataatg
    11581 caaaaactgt atgagcatca taaagtactt acttatccta gaactgattc tagatattta
    11641 acatcagata ttgttgggac attaaaagat agaataaaag ctgtaaatat tagtgattat
    11701 tcaaggattt gtaataaatt attaaaaagc aatatcacag ctaataaatc gtttgttgac
    11761 aattcaaagg taacagacca ccatgcaata ataccaacag aacagaaggt attcatgagt
    11821 gattttagtg acaaagagcg aaaaatatat aatcttgtag taaaaagatt tttagcagta
    11881 ctatctcctc cttttgaata tgagcagaca actataagtg caactgtaga aggagaaata
    11941 tttaaggcta agggaaacaa agtaataaat ttaggttgga aagaaagtta taaagatatt
    12001 gaagatgaag atgagtatga aagtatacca aatgttttag aaaaagatat acttaaggtc
    12061 aagggattaa agataacttc aggaaaaaca aatccaccac catacttaaa tgagggaaca
    12121 ttattgactg ctatggaaaa gaatagtctg gggacagttg ctacaagagc agatataata
    12181 gaaaaattat tcaattcatt tttaatggaa aagaaaaata aagatatata tataacatca
    12241 aaaggaagac aattattaga tttagttcca gtagacttaa agacaccaga acttacagct
    12301 tcatgggaaa agaaattatt ggatatttca aagggaaaac ttagtaaaaa tacattcata
    12361 aatgacatga aagattattc aaaagatatt gtactagaag taaaaaatag tgataataaa
    12421 tttaaacatg ataacttaac taaaaataga tgtccagaat gtggtaaatt tatgttagag
    12481 gtaaatggta aaaaaggtaa gatgttgatt tgtgaagata gagagtgtaa tactagaaaa
    12541 actatatcac aagtaaccaa ttcaagatgt cctaactgtc ataaaaaatt agaacttcgt
    12601 ggagaaggtg aggggagaat cttcacttgt agttgcggat acagagaaaa gttgtcttct
    12661 ttcaacaaga gaaagcaaga agaaaaacag aaggcatcaa aaaaagatgt caatcaatat
    12721 ttaaaaaatc aaaataaaaa tcaagagaca tttaataatc catttgcaga agcattagct
    12781 aagctaaaaa acaaataatt ttaaagttat tattatacac aattataact ttaactgtat
    12841 attagttata tgtactgata taatatagtg tatagtttag gcatgaaagt agggctgtta
    12901 aatggattta agtggcatat ttaaatacta ttgtaaagag tgtgaaaata catggaataa
    12961 ttcgagtgtt gaattatttg agaatataga aacgtatagt aaagattcac aaaaaaagag
    13021 ggaaaaagaa ttagataaat tgatgaatac aatatcagtt catttagaga ggtatccaag
    13081 tgatgctgta ttgagaaaaa tgtgggtaaa aaagggcgag gttttcttac aaaagacatt
    13141 ggaaaaagaa aatattttta agttagaaaa aatggatgta gaggatagaa aaaaattttt
    13201 agatataaca aaacagttta ttagagatgc tagaaaattt gatagtgatt taccgatagg
    13261 tgatattatg caagctatga ggaatgtatg gatttcaaat gcattacaat tattatttgg
    13321 taaagaagta tattattcaa aagctaactt tgcatatagt atgttatatc catatactga
    13381 taattattta gacaatacaa gtatagataa aaatgataag attttattta ataactggtt
    13441 agaaaaaagg ctcctgggag aacacattaa atctaaggat tatcatgaaa gtaaagtatc
    13501 tcaaatgata gattatattg aaagtgtata ccctagagaa aagtttacag aagtctatga
    13561 atcgttatta ttaatattta aaagtcaagt aaatagttta aaacaacatg gtaaggaaaa
    13621 tcatttgtgt aaagaagatt tattatccat ttctattgaa aaaggaggtt catccgtttt
    13681 agtagatggg tatttaataa gtggattgat gacaaaggaa gaaatagagt tttgtatagg
    13741 atatggattt ttattacaaa tatctgatga tttacaagat ataaaagaag atttaaaata
    13801 caaccataaa actattatta cagagatggc aaaagggggt actttagata aagttgtaaa
    13861 taaactaata aattttacta ttgagttaat agatagtttt aaaattaata ataaaaataa
    13921 atctgtaata actatgataa agaatgattg cttaatgtta attttatttt ctgtagttta
    13981 taatgctgaa tttttttctg taggatatat aaaagaagta gagaaattta ttccatatac
    14041 aatagattat tcattggaga ttgaagaaaa aataaaagaa aaatttaaaa atatagatgt
    14101 tttaaataat gaaaatgaat ataaagaaat gattgatatt atttgttcag agtagaaaaa
    14161 aagggagtgt cttagaatag actttttagt ttatgaggca ctctttttgt ataaaatcaa
    14221 atgtattttc atcattctaa caattaaaaa gaggcttatc caatttgaga cagccctttt
    14281 taatttaaat aaaaatgtca aggttaaact atttaaatgc agtaataatc ccattatcat
    14341 gtaagttttt tatgattgta gctgcgatag ggaatccaaa taatccagct attccaaata
    14401 gttgagctcc aacaaacata cacattaatg ttattaaagg atataggcca atttggtgtc
    14461 ctactacctt tggttctaat atttgtctaa caacagttat aattatgtat agtattaaaa
    14521 gtcctattgc taaattaata tttccattaa tgagtgagat aatcatccaa ggtgtcaaaa
    14581 tacttcctgt acccaagact ggcaaaatat caacaagtgc tatgataata gatatagtaa
    14641 atgcattttc aacctttagg atagtaagtc caataagtag ttctatgaat gtcactgata
    14701 gtagtattgc ataggctttt agtagtttta aaagagcatt aatactgcta ttttttatat
    14761 ttattatcat aaattgactt ttttctggaa attgttttaa gataaagttt actatttttt
    14821 gataatcaaa agtaaagaaa aaagaagcaa ttaaagtaat aataagtttt aacataaatg
    14881 caggtgtttt acttgcaata ctagcaatag cattgagtgc atttgttgaa atagaagcta
    14941 ctaaagacat catagattga cttatattat cccaaccaag tattaatgct aggttaacat
    15001 taggtgtact agcatctatc tttgaaaata aagtatttag cgttggttgt atacttgatt
    15061 tatatatttc aggaagtcta aaaaatacat cacttacact tgcaaatatc ttcacaccaa
    15121 agctaactaa taaaaatata cacaacccat aaaaaactaa aagtattact atagaaataa
    15181 gtgtacgatt taaatttgta ttatttgtaa taagtcttat aataggtctt aagatggatg
    15241 caatgataaa actgattaca aaaggcatta ataatggcaa taagaatttt aatacaatat
    15301 atacaatact tgttattaaa actacatatg taaatttaat tataaaattc ttttgctttt
    15361 gaatgtccat aatataaaac ctccaatgta tttgatatta ttctaaatat ggtctttttt
    15421 acagaaaata tactactgta tattgattat aaactataaa gcctatgtaa agtaaataaa
    15481 ttatgtaaaa tttaggttaa ttttgtataa agagatactt ttatatttta caattaataa
    15541 ccaaatacta aaaaatattt taaataaaga acttaaaatt gtgataaaat taatttatag
    15601 atattaaaaa aagtataata tatggttttt aagaggtgct aagatgaaaa attttagaga
    15661 tttaggtggg aacaaaactg aggatggcag aactgtaaag aagggattat tttataggtc
    15721 tgcaaaatta tcaaatctaa gtgaaaatga tataaagatt ttaaaagagt taaatattaa
    15781 atatatattt gattatagaa gtgatgaaga agctcgtaaa catccatcaa caataatatc
    15841 aaatataaaa aatataagaa taccagcaat gagagagtta gaagaatcag gaggaagttt
    15901 tggttctatt gaagatatga ttgatggatt atttgaaaaa gatggtgcat ttaatatgtt
    15961 aaataatagt tactataatt tgcctataaa taatccgtca tataaaaaac tagtagaact
    16021 tataagagat tattctaatt taccaatatt aaatcattgt acagctggta aggatagaac
    16081 tggtgtgggg agtgctataa ttcttatgat tttaggggtg tcgagagaga atataatgaa
    16141 agattatcta aaaagtaatg attttgcaga taaagagata gaaagattta tagaatataa
    16201 acctaaattt aaggatattc
//
LOCUS       DAEMAE010000078        16147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEMAE010000078 DAEMAE010000000
VERSION     DAEMAE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.080"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(198..989)
                     /locus_tag="KPH93_001926"
     CDS             complement(198..989)
                     /locus_tag="KPH93_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1688746.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIEDEYKGSIVPAF"
     gene            complement(1025..1681)
                     /locus_tag="KPH93_001927"
     CDS             complement(1025..1681)
                     /locus_tag="KPH93_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688747.1"
                     /translation="MNSLIDFLTTLFPSALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALVILVQIIQGVGNLAYKKLK
                     "
     gene            complement(1674..2639)
                     /locus_tag="KPH93_001928"
     CDS             complement(1674..2639)
                     /locus_tag="KPH93_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG1688748.1"
                     /translation="MISIKNVNKYYGKVQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGNVFVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERRTVFDNVALP
                     LECFGYSKEEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(2745..2851)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3140..4084)
                     /locus_tag="KPH93_001929"
     CDS             complement(3140..4084)
                     /locus_tag="KPH93_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG1688749.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHSIKSKVLELYNHHCI"
     gene            4195..4662
                     /locus_tag="KPH93_001930"
     CDS             4195..4662
                     /locus_tag="KPH93_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688750.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTVMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(4830..5429)
                     /locus_tag="KPH93_001931"
     CDS             complement(4830..5429)
                     /locus_tag="KPH93_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688751.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDNVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            5675..6271
                     /locus_tag="KPH93_001932"
     CDS             5675..6271
                     /locus_tag="KPH93_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG1688752.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(6365..7324)
                     /locus_tag="KPH93_001933"
     CDS             complement(6365..7324)
                     /locus_tag="KPH93_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1688753.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNENIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(7358..8089)
                     /locus_tag="KPH93_001934"
     CDS             complement(7358..8089)
                     /locus_tag="KPH93_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688754.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATNGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(8082..8852)
                     /locus_tag="KPH93_001935"
     CDS             complement(8082..8852)
                     /locus_tag="KPH93_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688755.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKIYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDDKLIEVGKSFNL
                     SRKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(8937..9647)
                     /locus_tag="KPH93_001936"
     CDS             complement(8937..9647)
                     /locus_tag="KPH93_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG1688756.1"
                     /translation="MFKFDVTKELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(9828..9980)
                     /locus_tag="KPH93_001937"
     CDS             complement(9828..9980)
                     /locus_tag="KPH93_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG1688757.1"
                     /translation="MATFIIAAIVVVLMALAVMHMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(10006..12162)
                     /gene="feoB"
                     /locus_tag="KPH93_001938"
     CDS             complement(10006..12162)
                     /gene="feoB"
                     /locus_tag="KPH93_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG1688758.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDKSKEAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKAILNSIEEITRNCEDELDDDSESIITANRYEFISSIISNIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIIAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIVIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(12262..12483)
                     /locus_tag="KPH93_001939"
     CDS             complement(12262..12483)
                     /locus_tag="KPH93_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1688759.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(12496..12708)
                     /locus_tag="KPH93_001940"
     CDS             complement(12496..12708)
                     /locus_tag="KPH93_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1688760.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(13029..15926)
                     /locus_tag="KPH93_001941"
     CDS             complement(13029..15926)
                     /locus_tag="KPH93_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1688761.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPELSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIGELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMVLSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFLCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
BASE COUNT         5274 a         2862 c         1694 g         6317 t
ORIGIN      
        1 ttagctagta tatagacctt attcttactt aaagttcctt tagattccat attccccacc
       61 acttcttcat ttttatatag ttttacctat aaatatgtta ccacaattct tccaaaaaat
      121 ttgtcacttt ctagttataa aaaaaaatga gtaaatattt ttaattttac tcattttgta
      181 gattttgtat attcagttta aaaagctgga actatacttc ctttatattc atcttctata
      241 aattttttaa cttctttact attcatagcc tctgataaag ccttaatctt atcactatct
      301 ttattatttt ctctacaagc tattatattt acatatggag aatcacttga ttctattact
      361 attgcatcct tagtaggatt tagattagct gttaaggcgt aattagaatt tattactgct
      421 ccgtcaacat cttttaatac tgttggtaat tgctctgcat tcatttcttt tattactata
      481 tttttaggat ttttagttat atctttttta gttataagct ctccatcttt tacttctatc
      541 aatttatttt ttgccaataa tttaagtgct ctagctccat tagttgcatc atttggcaca
      601 gctataacag ctccatcttt taattcatct aatgacttaa ctttttctga atagaatccc
      661 attggctcta tatgaacttt agatgtataa gtaagtttat atcctttctc cttcacagtt
      721 tcctctaaaa atggaatatg ttgaaagaag ttagcatcca aagacccttc atctaaagcc
      781 gtatttggaa gtacataatc atcaaatatt tttacttcta aatcatatcc tttttctttt
      841 aacaaaggtt ttgcaacttc caatatcttt gcatgtggat ttgatgaagc tcctactagt
      901 atttttttat cctctttgtt tgaacaacct actgctgata ttgctattgc tgacaccaat
      961 gctactgata ataacttctt taatttcatt tttactcccc ctctttaaaa tgtttataat
     1021 taatttattt tagtttctta taagccagat ttcctactcc ctggattatc tgaactaata
     1081 ttacaagtgc tattacagta tataccatta ctgctgtatc aaatctttga tatccataaa
     1141 taagcgctat attacctagt cctccagctc ctactgcacc tgccatagct gtataaccaa
     1201 gtatagatat tattgataat gtaattccag atactattga aggcatagct tcttttatca
     1261 taactttaaa tactatttgt ctttttgtag ctccaaaaga ttttgctgct tctatcaacc
     1321 ctttatcaac ttcatttaat gagctttcta taattctagc gataaacggt gctgccccaa
     1381 tagttattgg cactattgca gcagtttccc ctatactagt tccaacgatt aatctagtta
     1441 ttggaatcat tgcaactata agtatcataa atgggaaact tctaaatatg tttactataa
     1501 atcctaatgc actatttatt gtgctatttg gttttaatcc atctggtttt gtaactacta
     1561 atatgattgc taatataaat cctaatattg ttgccactat agttggtact ataaccatat
     1621 acaaagtttg taacaatgca cttggaaata gtgttgttaa aaagtcaatt aaactattca
     1681 tttatcattt cctcccatct tatgccttta ctagtcagat attgttttac ttgctctcca
     1741 cttttatctg aaatgtttat aataagtgaa cctaaaatat cgtctttaaa ttgttctaat
     1801 ttcccaaata ttatagacac atctatattt aattctcttg ccatagtcgt aataatagct
     1861 tcattagaaa tatctttagg gaatagtatc tttatatttg ttccttttgg taatattata
     1921 ctttcttcac ctattaattt tctaagccct ttagtatttc ttaagaatat ttcttctgta
     1981 tctcctactt ctaatacttc tccattttcc attattgcta ctcttccaca aatctgcttt
     2041 ataacttcca tttgatgagt tacaactatt attgttattc ccaacttttt atttatatcc
     2101 tcaagcaatg aaagaattga cttcgtagta tttgggtcta atgctgaagt tgcttcatca
     2161 caaagaagta cctgtggatt tagtgctaaa gctctagcaa ttgcaaccct ttgtttttgt
     2221 ccgccactta gatttctggg tttatcattt ttcttttcag atattccaac tacttctaat
     2281 aactctaaaa ctcttttttt aatttcttcc tttgagtatc caaaacattc taatggcaat
     2341 gctacattgt caaatacagt tcttctttca agcaatgaaa agtgttgaaa aatcattcct
     2401 aactcttttc ttaaatctct catttgcttt tcattcaatg actttacttc tttatcagaa
     2461 acaaatacat tgccttcttg gtattcttct aatccgttta tacatctaag tagtgttgat
     2521 tttcctgcac cactatgacc tattattcca aaaatttctc cagattctat ttcaatactt
     2581 acatctttaa gcacttgaac ctttccataa tacttattaa cattttttat gctaatcata
     2641 aaatttcctc ccatatattc tgctcagttt ttatgtttat cctctcttat gtattacaac
     2701 aaaataaaag cccgagtata aactcaggct taaaaagcta tttatactca tctatcaact
     2761 atattagttg caggatttag caccaatgta tataaaatat actggttgcc gggtttcaaa
     2821 gggccagtcc ctccaccact cttgataaga cgttatttaa tttaattcta ttatcatttg
     2881 ttgctatgtt atatatgata tattcattaa tagtatttgt caataaattt atttaaattt
     2941 tttatttttt attatcgtta attaactatt tttaattagt tttctagctc ctaacacttt
     3001 atattttaat gtctatataa ctatataaca tcattataag aaatatatta aataatatca
     3061 atatatctaa atcatctgta aagtaaatta agaaaattaa cattttctcc aaactctatc
     3121 ctatcttaaa taaaataatt taaatacaat gatgattata tagctctaat actttagact
     3181 ttatcgaatg tttaagttct ataggttcta ttattgtagc attttttcca aaagttaaaa
     3241 aatattcagc cacccaatta agtgtagatt ttggtatata catatctatg tatccaccct
     3301 tagacaaaat tttcaatcct cttgccaaca aaaattctgt ttcacaacgt tttactccct
     3361 catctgtcag attaatttta atatgataat ctgtacctac ttccatattc ttaagataat
     3421 catgtataga agataaaacc ttatatttct tatttgataa gttttctata ctcaaaatct
     3481 tcacaattct gtctgcacga aattccttaa cagattctga ttttatacag taagctggac
     3541 aataccaaag tccattcata gcatacaaac caataggaac aatggtacgt atactatttt
     3601 tttgctttga tgaatattca attttaattg taaattcatt cattactata ttaaacaatt
     3661 ccttcaataa tggcgttgag caatgcctat ctggtgtcca aaaaacaaat ttacgtctca
     3721 tattctcaat attatattga atatcatttg gtatacaatt taaaaatttc tttaaaacag
     3781 aaatagtttc ttgctcaaat ggtaaatcat tataatactc tagtgactga tatgcaaaga
     3841 atatagattt tacctcactc tctgaaaatg taattggtgg aagtatacgt tctttaagta
     3901 catgatatcc tccagccgct ccaacttttg aatatagtgg aaatccaaca ctttccaata
     3961 cctgtaaatc tcttagtata gtacgttttg atacagaaaa ttcttctgct aactctcccg
     4021 ctgtaaaact tctttttgca tttattgtta tcatcagttc aattaaacgc tcagctcttg
     4081 acatattcat actcctaatt taaatatagt gacaataatt gtcaccatta tatgataaga
     4141 tattcttgca tctaaagcaa taataatatt aaagaaagaa gggattgata ttttatggaa
     4201 aatgatacta ttaaggctga tgatattctc aattattgtc tatcaaactt agatgatgtt
     4261 gtactaatgg atagttgggg ggaacgagca atttactaca atcctaatgg tgttttaaag
     4321 cgaggggtat atgttcttac cattaaagaa aaagacagta ataatgataa aggttcgtta
     4381 gttagtcgtc caaatgtata ccgtgtaaat ataggattaa aaaaagaaac ttttattgaa
     4441 atgtttggat atattccaaa gcgtccaggt gtagggcaaa tagttgatat ggattttgat
     4501 tttacaaaat tggacacagt catgccacat cctatctact catggatggg atggatatgc
     4561 gttctaagcc ctactgaaaa gacatttgag aactttaaaa ctttaatagg agaatcttac
     4621 aattttgcaa aacaaaaatt taaaaagaga aaaaacaagt aaaaacataa aaaaataacc
     4681 tctaaataaa ctataatgaa aatatgaggt tacaagataa aaattagtat cttgataaca
     4741 tacaaaaaag cgaagccaaa aaatatatta attacaaggc ttcgctatat ttcaaatatt
     4801 tatttctaac tttaaaacta tttactctat tattttttat tatttcctgt tattcccaat
     4861 ctatcattca tatcttttat catttttcta aaaccactac caatatcgtt tccaacatct
     4921 tcagttccat gaactagttt cataaaatca ttttgacctt ttttcatatc ttcatcagat
     4981 acattatcta atttttctat tctctcatct atatctttta ctaatctttc cccttcagcg
     5041 attaggtcat cattcattct ctttaactct gtatcttctg ttttaccgtc ttttttaaga
     5101 gatgttacaa atgattgtat tttatctcta ctatctttat atgcagcctt tacatcactt
     5161 agatattttt catttcctgg atattcttta tctttaaact gattgtttat actttctgga
     5221 gtatcataaa tagaatatct atttagacct gctatataat cattataact tgttgaataa
     5281 taattcatat atccttcatt attatttaca gattcattag gattttttcc tgcattttca
     5341 tttggttttt cattatctct agcacatgca gttattccta tagctaatac taatgctaat
     5401 cctacaaata aagttctttt taatttcatt tttattaccc tcctagatgt tttgtactac
     5461 aaaaaatttt aataattttc tttcttaatt tagtatttct atatttttat tttttattta
     5521 ctaaaattaa acaagttata atttattttt ttggaaatca taatgaataa ataattaaat
     5581 aaaaaaatta taattttatt attgatattg attatcattc atagtataat ctaattactt
     5641 gatattaatt ttcatttgaa ggaggttatc ttttatgtct tgttgtaatt cacaattttg
     5701 ttgtaaaaat aaaattgaat catcatatat aaagtggctt ttagaagttt taggtccagt
     5761 ctttttaggc tccaaacctg ctgaaataat aaatatttcg tttactgatg taaatagaga
     5821 agaaaaaatt aatgatattt ataaatatct atataaatgt aagaaaatag attttatagt
     5881 tattgataaa gaaaaaaaag gattaaaaat cttatttgta aataaaaaag cattatcaga
     5941 aaaattaaaa tgtaaaaaaa cagtaaactt tttaaaattt ttaggataca aacaaaatac
     6001 gaatgttaat gcttatttag agcatttagt agataaactt aagagtgatg tttttcctga
     6061 tgaaataggt atttttttag gatatcctct taaagatgtg attggattta tgggatatag
     6121 taattatgaa gtttctatga ttaaatattg gaaagtttat ggtgacacaa aacaatccga
     6181 ggacacatat tcgaagtttt tacttcaccg taagaaaatg agaaaattac tagactacat
     6241 aagtgtagat aaaatagtaa gttgttttta attgtgtttt tgtataataa taaaaataca
     6301 ccctctttta ctatacagct tccataataa gggtgtattt tctactttaa tgttttaaag
     6361 tattctaatt ttgattgtac aacatatctt taatatttat tttcttttgt tgcattccat
     6421 tagacatcaa aaattcctgt gtatcttcta attcttttat atccttgtcc gttattttag
     6481 aactaaagtc ataccaagaa tacatttctt ttgtttcatc taaagataaa ccaacttctt
     6541 tagatacaac atccatcact tcatctttgt tttcattcat atattttaaa gtttcttcat
     6601 gaaccttcat aaatcttttt actaattcag gatatttttc agcaaaatca tcacttacag
     6661 cagtaactat tattccactt actaatcctt ctccatttac aatcattttg gcacctgaat
     6721 ttattgtttt caatgccact ggacctgcaa gcattgctac atcagcactt ttactttcta
     6781 atgcagaagc agcactagga atatccatat ttacatattc aacatcatct atagtatagc
     6841 cttctttagc aagtgcagca atcaacagtt gatgaagtat agtccctttt ggacctacaa
     6901 tttttttacc ttttaagtct tttatagatt taatattttc attattagtt ataagcatat
     6961 atccttttgg tgaacgacta taaatattcg taattttaag accaacacca tttgatgcag
     7021 ctattattgc agacgttcct cctaatgcat gtaaaaagtc tagctcacct gctgctaatg
     7081 cttgagtttg ctctggtcct gttgtcaatt catgaaagtt gacttttata ttgtcctttt
     7141 taaattcctt tccaaataaa tcatcctttt tttctaatat actaggaaca ttaagtggtg
     7201 attttacata agttaaattt atttccttag gtaaactagc ctcttttttt gcaggttcat
     7261 ctttcttatc atttgttgtt gaacatccta cagttccaac caacaccatt attaaagatg
     7321 tcattactgc taaaatcttt ttgtttcttt taatcaatta aaactccccc aattccctta
     7381 atatttcttt tttcaagttt atataatagt ttttagttaa atctctatta tattcatctt
     7441 cgacactaaa ctgtttaatc cttttatttt tactaaaaac aattattttc tttgctattt
     7501 ccatagcttc ctcaatattg tgagttacaa atactacacc ttttttagta tttttatgga
     7561 tatttacaac ttctttttgc atttttcttc ttgtgaagta atctagtgct gcaaatggtt
     7621 cgtccatcag caaaatatcg ggattgaagg aaagcgctct tgctatactt actctgtgag
     7681 ccattcctcc tgataattcg tttggatatg agtttttaaa cttttcaagc ttcatcattt
     7741 ttaaatactt gtctaaatca actttatttc tattatcttt ttcagtatgt aaaagtatat
     7801 tttcacttac attcagccat ggcataagtc tactttcttg aaataccatt cctacttttg
     7861 gagtacattt tttatcatta ttaaaaaaat atacgtttcc attagtagca ttttcaagcc
     7921 ctgctaaaat tctaagtagc gttgttttcc cacatccact ttcgcctaat ataacagtta
     7981 tctcttcact agatatattt aaagatatat tatcaagaac taaatgctct ttattatcaa
     8041 ctaaatattt cttatatata ttctctagca aaaatccact tctaaccatt taaatcaacc
     8101 tgcttccctt tgctaaattt tgttattatc tttgaaaata aataatctga aagtatacct
     8161 aaaaatccaa taacaaaaat tcctaccaag acaacatcag ttctagacat atcttttcca
     8221 tcagaaatca aatagcctag tcctgaagaa gctgcaataa gctctgctcc aattatagcc
     8281 ctaaaactat acccaagccc caaactaagt ccaacaacaa tatccagcat agaattagga
     8341 attattattt tataaaaaat tctctttcta gacaaattaa aagatttacc tacttctatt
     8401 aatttatcgt cacaattttt tatgcctttc aatgtattta aaaatattgg aaaaaatgat
     8461 gccaaaataa taattataat tttagattcc tcacctatac caaaccataa aataagcatt
     8521 ggcaccaaag ctagtggagg tgtacttcgc ataaattcaa atagtggctt gaaatattca
     8581 tatatttttt tatttactcc aaatacaact ccaagtggta tagccatcaa tgaacttatt
     8641 aaaaaaccta ttaatatcct tcttacactg gcataaacat ttaagaaaat agaaccgtca
     8701 tatatcattt ttataaaagt atctaagact atttcaggag gaggcagaat atagctactc
     8761 catataccca aaacacatac tatcttccat aaaattaaaa ttattaaaaa aatataatat
     8821 cctttaaacc ttttatgaat tttctcaaac atataaaact ccttttaaca tatctatccc
     8881 tgctaccctt tttatttttt aatatcttaa ataataatca agctatatca taagatttaa
     8941 tctatgccaa gtcttaatcc acctgaactc attctatcag aaggtgtaga atctgttttt
     9001 aagccagaca atctttcaga tggttgtata atgactattt gtccaggttc aacaaattcc
     9061 atatgataag attctcctga agtctgaccg ataaaagtct tccatgatag ttttgatact
     9121 ttaggatatg gttctcttcc agtccaagct actaatgcat ctgggtcaac acaacaaggt
     9181 ccttcaagaa ttagaggatt accatctgtt attatagcaa catcttgatt ttgtcctgtt
     9241 ttatttacta aatgtgtagt aaaaagacct ttttgagata ttacccctga accaatcaat
     9301 tttacactgt aatttaattg ttcagaaaat gccaataagt tttcactctc aacataaatg
     9361 gaatcacctg ggtcaattga tattacatct acatggtaag cattttgagc taggtaaatt
     9421 tcaccttcac cttcaaccac cattattggc atttgttctc ctgtcaatga cctctgaaca
     9481 tgacctaata atgctcttcc caagccacca ccattacttg gacctagcag caatttttca
     9541 aaattaaaat ttcctttaaa tgctaccata gcaccttttt ttgcaaaaaa tttaccactt
     9601 ccccttgcta tacacataag ctcctttgta acatcaaatt taaacataat attcactcat
     9661 attgcttaat ttagcaataa tccctcctaa tcatatattt gtctataatt atacaataaa
     9721 attacaacat agtctaactt acttcaaata tgaaatttgt tatttatata aaaaataaga
     9781 acctgataat aaagcaattt aaacttttta tcaggctctt atcctattta tctcttagcg
     9841 ctatgacaac tcttactact cgagcatcca gaacaaccac atccacaact gctaccattc
     9901 ttttttgaat ttttaaccat atgcattaca gctagtgcca ttaatactac tacaatagct
     9961 gctataataa atgtagccat attcatcact ccttatttat atgaattaat ttttcatata
    10021 acttaattta cccatttttt cttttttcat atttttattt gaataagtta aaactacaaa
    10081 gactactgct actgctataa ctatagatat tattgcccca gcaatacttc ctccagtacc
    10141 taaaactaaa cttccaactt gattgataag aagagcaatt atatatgctg ttaaagtctg
    10201 gaatcctaat gtaatccatg tccatttcca tgaacccatt tctcttttta tggctccaat
    10261 agctgcaaaa caaggtgcac aaagcatatt aaatgccata aatgcaaacg cactagctac
    10321 tgtaaacata cttgcaactg aagcaagaag tgttgggtct tgctctgttg catctgatat
    10381 accaaataaa actccaaatg ttccaactac attttcctta gctactagac ctgtaactgt
    10441 tgctactgat gattgccaat ttccaaatcc aagaggagca aatattggtg caattatatt
    10501 tcctaagcta gctaatatac tatctccagc atctaccatt tgtaacgacc aattaaatga
    10561 ttgtaagaac cagattactc cacaagcaac aaatataatt gttcctgctt tgattataaa
    10621 tgcttttcct ctatcccaca tatgtattaa aacacctttt gcacttggaa tatgatattg
    10681 aggtaattcc ataacaaatg gagatggttc tcctgcaaat aaactagttt tctttaatat
    10741 aattccacaa atgattatca ttgcaatacc taagaaatac attgatggag ctacccatga
    10801 agcacctcca aataatgccc ctgcaaataa tgctattatt ggaatcttag ctccacatgg
    10861 aatgaatgta gttaacataa tagtcatttt tctatctcta tcattctcta tagtacgagt
    10921 tgacataact cctggaactc cacaacctga acttataagc attggtataa atgattttcc
    10981 tgaaagacca aacttacgga aaattctatc cattataaaa gccacacgcg acatgtaccc
    11041 acaatcttcc aatattgata ataataagaa taaaagcatg atttgtggta caaatcctaa
    11101 tactgctcca actccaccaa ttagaccatc aactacaagc ccttgtaacc actctgctac
    11161 atttaaagaa gctagaaaat tacttacatt accttgaata atttctccaa acagtacatc
    11221 atttgtccaa tcagttgcta tagtacccaa tgaacttact gctatatagt aaactcccca
    11281 cattataagt gcaaaaatag gtaatgctag tatacgattt gttacaatct tatctatctt
    11341 atctgataca gtttccttac cttttctatt cttctttatt atatttgaaa ttattgaact
    11401 aataaattca taacgattag ctgttattat actttcacta tcatcatcca attcatcttc
    11461 acaatttcta gttatttctt caatactatt caatattgct ttagaaatat caagtttttg
    11521 tataacattt tcatctcttt caaatagctt tatagctaac cagcgtgttt ctacatctat
    11581 atatggagtc ttttcttcta ttattttttc aattttttct attgcttcct tacttttatc
    11641 aacgaaaggt aatttgaaat ttaatttatt gttactactt gcaagttcaa ctgccttttc
    11701 agcagcctcc ataattcctt gacctttaac tgctgttact tctaccactg ggcaacctat
    11761 aacttcagat agttctttaa tatttatctt atcaccattc ttattgacaa tatccatcat
    11821 attaagagct ataacggttg gtatacctag ctctaaaact tgagttgtaa gatataaatt
    11881 tctctctata tttgatgcgt ctacaatatt tattactgca tctggcttat catctagcat
    11941 aaaattacgt gtaactactt cttctaatgt atatggagat aaagaataaa tacctggtaa
    12001 atctactatt tccacatctt tatttccttt taattttcca cctttttttt caactgttac
    12061 tcctggccag ttaccaacat attgtgatga gcctgtaagc ccattgaaca ttgttgtctt
    12121 accacagtta ggatttccta tgagacctat ttttattgac atttccattc cctccattat
    12181 tatttaaata catcactatt catgatatag aaaatcattc tcattatata attacattaa
    12241 ccctactcta attaatcttt attattccac aataattttt tctgcatctg acttacgaat
    12301 tgatagttca taatctctga ctgttacctc aattgggtca ccaagaggag ctacttttct
    12361 tataaatata ttaacgcctc ttgtaatccc catatccatt atacgtcttc gtgtagctcc
    12421 ttctccttct aactttttga cttttacagt ctctccacac ttaatatcct tcagtctgtt
    12481 catgtcatta cctccttata ttattattct acttgccatt tttttatcta atgcaactct
    12541 agtatctttt acgtctatta ttaagttacc atctatctta gaaactattt taacatttgt
    12601 acctactaca aaacctatac tatttaaaaa agtcctcgtc ttatcacttc ctgaaatttt
    12661 ctttataact acttcttcac ctgtatttga tagtatcaat ggcatcatat aatatccctc
    12721 ctcttaaact aatttaaatc tacaagcaat cgttatctta tatataaatt acaatacctt
    12781 aaatcttaat ctattaaaat aatatcctat tttttccttc tttttacaat tcctaatttt
    12841 gatttaagtt tatcacaata gtatattttt tgcaagtgat tattgtattc ttttgatttt
    12901 ttttctcatt tgatttgttt tattaataaa atcgtaaaaa ttaatacaaa aataaagatg
    12961 ctctaaaata gtatactcaa ctaatattaa agcaccttta ttatatatat cattacttac
    13021 acattgcttt attctttttt aaaagcgatt ttttcaaact cttttacagg cattggctta
    13081 gaaaacaaat atccttgtac catgtcacac ccaatttgct ttagaaattc tacttgcgaa
    13141 atagtctcta caccttctga tattactttc attttcagtt cctttgccat tttaatgaca
    13201 ttagatataa caattttttc attattactc tcatcttttt ctgtaaaaaa tgctctatct
    13261 agttttaata tgtcaacagg caattctttt aaaagattaa gagaggaata tcccgtacca
    13321 aaatcatcca tagagattac aaatcccttt tctttcaaat tgttcattgt atcaagtaat
    13381 ccttctatat tatctaatac tgctgtttct gttagctcta attctataag tcttgtcgga
    13441 attttgtatt tatctacaat taatttaaac ttctctataa aattactatt tacaaaatgc
    13501 attcgagaca agtttacaga tataggtact actttttgtc cttcatccat ccattctcta
    13561 atctttttac acacttcctc aaatacatag aaatcaatat ttacaataaa accattcctc
    13621 tcaaatacag gaataaattc tattggtggt attagacctt tttgtggatt atcccatcga
    13681 acaagagcct ctgcccctat aatttgataa tctgaaagac tatatttagg ttgaaaataa
    13741 acaataaatt ctccattatt aagagcatct accattgaat tttcaatttc tttctctttt
    13801 aatatttttt tatgcatttc cttatcgtaa aaagcaaatg aatttttatg accacctttt
    13861 atagtttttc ttgcagtatt agctctatcc ataatagtat ttatgtcatt atcactttta
    13921 cttattttat atataccaca atctaaaatc aatttataat agtttatttt aggattacta
    13981 agtatttgaa ccttattgta aatattgttt aaccttgttt taatatcatc atctgtctta
    14041 tacttaataa gaagaacaaa atggtcagca gaaactcttg caaaaatttc atcttccttt
    14101 aattcacttg caatagtatt tgatatatgt atcaaagtat catttcccat atcgtatcca
    14161 aacatatcat ttatatattt aaatttgtca acatctatat aaaacaacgc atattcttct
    14221 ggattatttt tagcaaacag tttattcgaa tttattttaa atttatctat actatctgct
    14281 cctgtcaatg ggtctgtata tgctatcttc tttagataat cctctttctt ctttttatta
    14341 taaattatat atttaatagc aattaagaat aaaatacata aaaaagaaat aatttgtatt
    14401 gaatattctt taaaaatcac accaaatggt actttataag gtttgtcaat agtattagaa
    14461 tatatacaat cacttaattc ttcttcagta attttgtcta ttgatttatt taaaatagat
    14521 actaatatag ggtcagtttt atttgatacc cctatacgca tcccatagct tgtatctaaa
    14581 acagatataa cagataaatt ctcactcttt ccttctctta aaagttcatc aaactgataa
    14641 gaactaagta ccattaagtt tgctttacct tcatttatag cgtttagaca actttctata
    14701 ctcttataag atgttgtctt agcattttca aaactttttt ctatataaga atcaatagac
    14761 ttataactac tcgttaaagc aatatttaaa tcattattga gattggtatc cagttttcct
    14821 accataatca ttggaatatt tatgtatgga tttgtcaatt tcatattaaa tcgttttgcc
    14881 ttatcatttt cattgattga gccacatagc atatttgcct taccactttt tatgtaatca
    14941 agactctcat tataattttt tgttttaata tattcaaatt ttataccaca tttttttgat
    15001 ataattgaca ttaaattgga acttatacct ttaaacgaat tactttcctt atcataatac
    15061 tctacaggag accactctga atcatatacc atagtaagtt ttgggtttgc tttaataaaa
    15121 ttcatttctt gccgagtaaa tgctatggaa ttattgacct ctcttttgaa atatttttta
    15181 tataattcca actcataata catatcattt atttttaatt cttttaaagc ataatctaat
    15241 tcttttacca aattaggttt atcttttgca gtagcaaaat aaaaaggctc tactgaaaat
    15301 ttgctaacta ttttcattcc tttagcattt ataatacttc cacagacaaa tgcatctgcc
    15361 tttttttcat tgacagactc aattaattca ccaatagaat tacagtatat atattcaact
    15421 gaaaaattat tttcttctct atacttctca aatgcaatat tatgaaagtt tcctctaagt
    15481 acagctactc tcattccctt aaaggaactt atatcttcat aaaacatatt attgttacta
    15541 tcttttgtat atagtgcact atattcatat cctgaactta gttcaggaaa attaaatagt
    15601 ttatttcttt catcattctt ctgtaaaggt ccaagtaagt ctatttcacc tttttctagt
    15661 ctgtctaaac attcttccca agaccctttt acaaattcat attcccatct tgtatatttt
    15721 gagatttctc ttagataatc ataaccatat cctgtataag aatgctcttt tgatgaggat
    15781 attattccgt agtcatcaca atatccaact cttatttttt ttgaattttc agactcatca
    15841 gcaaagcata gtatattgtt aaaacatgat ataataagta aaaatatcaa agaaagtatc
    15901 aagaagtgct ttttaaatgc tttcatattt aaaaacctcc aatttgccaa cttactttaa
    15961 cttatttggt tttctagttg taactacttt tattgtaatg gtaaatatat caaattgcaa
    16021 tattctcata taatattctt attcaactat aataaattta ttattatcta tttaaatacc
    16081 ctatctaata ttaatttctt ataagtcata aaagtataat taaaactata attctagtta
    16141 ttaatat
//
LOCUS       DAEMAE010000079        15942 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEMAE010000079 DAEMAE010000000
VERSION     DAEMAE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.081"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="KPH93_001942"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="KPH93_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG1688762.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="KPH93_001943"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="KPH93_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG1688763.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="KPH93_001944"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="KPH93_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG1688764.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1232..1657
                     /gene="rplK"
                     /locus_tag="KPH93_001945"
     CDS             1232..1657
                     /gene="rplK"
                     /locus_tag="KPH93_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG1688765.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1728..2426
                     /gene="rplA"
                     /locus_tag="KPH93_001946"
     CDS             1728..2426
                     /gene="rplA"
                     /locus_tag="KPH93_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG1688766.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2478..2606
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2649..3155
                     /locus_tag="KPH93_001947"
     CDS             2649..3155
                     /locus_tag="KPH93_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG1688767.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3213..3578
                     /gene="rplL"
                     /locus_tag="KPH93_001948"
     CDS             3213..3578
                     /gene="rplL"
                     /locus_tag="KPH93_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG1688768.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3949..4737
                     /locus_tag="KPH93_001949"
     CDS             3949..4737
                     /locus_tag="KPH93_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG1688769.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VSGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5208..8924
                     /locus_tag="KPH93_001950"
     CDS             5208..8924
                     /locus_tag="KPH93_001950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG1688770.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRKLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIMKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8966..12451
                     /gene="rpoC"
                     /locus_tag="KPH93_001951"
     CDS             8966..12451
                     /gene="rpoC"
                     /locus_tag="KPH93_001951"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG1688771.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12740..13162
                     /gene="rpsL"
                     /locus_tag="KPH93_001952"
     CDS             12740..13162
                     /gene="rpsL"
                     /locus_tag="KPH93_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG1688772.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13285..13755
                     /gene="rpsG"
                     /locus_tag="KPH93_001953"
     CDS             13285..13755
                     /gene="rpsG"
                     /locus_tag="KPH93_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG1688773.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13804..15870
                     /gene="fusA"
                     /locus_tag="KPH93_001954"
     CDS             13804..15870
                     /gene="fusA"
                     /locus_tag="KPH93_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG1688774.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6237 a         1995 c         3247 g         4463 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttatgctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagtttg tttggatact taaaagaaac
      481 taaacaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaagtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtt ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtgccattaa
      961 gtgaagacga agtcaaagct atggggatag acacaacaga ccctaaagta gtaaatagcg
     1021 atattgattt tgaaatagga gatacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 ttatagaaaa atctgaggag gtgcgctcaa aatggctaaa aaagttatag gtcaaataaa
     1261 attacaaata cctgcaggaa aggctactcc agcgccacca gttggaccag cattaggaca
     1321 acatggtgtt aatataatgg gattcacaaa agaatttaat gctaaaactg cagatcaagc
     1381 tggaatgata ataccagttg ttataactgt atatcaagat agatcattca gttttataac
     1441 aaaaactcca ccagctgcag ttttaattaa aaaagcatta aacttaaaat caggttctgg
     1501 agaaccaaac aagaaaaaag ttgctaaaat gacttcagct caagtaagag aaatagctga
     1561 attaaagatg cctgacttaa atgctgcatc agtagaagct gctatgagta tgatagctgg
     1621 tactgctaga agcatgggtg ttgtaattga agactaatta aaagatacac ttttttttat
     1681 aaagtgggag gcgagaagcc gaaattaacc acaaggagga aaataaaatg gctaaaaaag
     1741 gtaaaagata tgcaggtgca ttacaaaaag tagatagaac taaattttat gatgcatcag
     1801 aagcgttaac attagtttca gatatagctg gagctaaatt tgatgagaca gtagaagcac
     1861 acattaaatt aggtgttgac tcaagacatg ctgaccaaca agtaagaggt gcagttgtat
     1921 tacctcatgg aactggtaaa acaaaaagag ttttagtttt tgctaaaggt gaaaaagcta
     1981 aagaagctga acaagctggt gctgactttg taggagctga agaattagtt caaaaaatac
     2041 aaggtgaaaa ctggtttgac tttgatatag tagttgctac tccagacatg atgggtgtag
     2101 taggtagatt aggtagagta ttaggaccta aaggtttaat gccaaaccct aaatcaggaa
     2161 cagttacatt tgatgtagct aaagctatag atgaaataaa agctggtaaa gttgaataca
     2221 gattagacaa aactaacata atacacgttc cagtaggaaa agtatcattt ggtggagaaa
     2281 aattaactga aaactttact gcattaatgg atgctataat aaaagctaaa ccagctgctg
     2341 ctaaaggaca atatttaaga agtataacag tagcttcttc aatgggacct ggagttaaaa
     2401 taaacccagc aagaacagct gaataataaa actgttgaca caggttgaca aaaatgttat
     2461 tatatcactt gtcaatgtaa aaaagaatag atttgccgta gatagtgggt gcgaaagctt
     2521 aatatcccgc cgaggtttta gataaattga gattactaaa gcttttgatg tctacttttg
     2581 gcaacaaaag ctttttataa tgttgcggta aatactaagc ctatttggtg aaggaggtgc
     2641 acatctatat gagaaaagct atagaaatta aatcagaagt tgtttctgag atagttgaaa
     2701 agttacaaaa atcttctgct gctgttgttg tagattacaa aggattaaca gttgaagaag
     2761 taactgagtt aagaaaacaa atgagagaag ctggtgtaga ttacaaagta tacaaaaata
     2821 ctttagttag aagagcagct aaagaagttg gtatagagca atttaatgat gaattattag
     2881 ttggaactaa tgccatagca tttggatatg atgatccagt tgctccagct agaatattaa
     2941 aaggattcat ggattctcat ccaaaaatga aattaaagat gggtattgtg gaaggtgcat
     3001 tctatgatga gtctaagatt gttgaaatgg ctaacatacc atcaagagaa gttcttatcg
     3061 ctaaattact tggtagctta aaagctccag tatcaaactt cgcatactta atagatgcaa
     3121 tagcgaaaaa ggcagaaggt caagaagaag cttaattgct taactaaaaa attataaata
     3181 ttaatttgaa ataaaattcg gaggtgctaa aaatgacaat agaacaaata ttagaagcta
     3241 tagaaaatat gaaagtttta gaattaaatg aattagttaa agctgctgaa gaaaaattcg
     3301 gtgtatcagc ttcagctcca gtaatggtag ctggtgcagc tgctggtgga ccagctgctg
     3361 aagaaaaaac tgagtttgat gtagtattaa ctgacgttgg ttcttcaaaa gttggagtta
     3421 taaaagcagt tagagaaata acaggattag gattaaaaga agctaaagaa gtagttgaca
     3481 atgctcctaa gacagttaaa gaaggagctt ctaaagaaga agctgatcaa ataaaagaaa
     3541 aattagaagc tgctggagct aaagtagaag ttaagtagtt tctttaaaaa gatacccatt
     3601 gggtatcttt ttttatttat aaaaaattat aaagaaagta tttttataaa ataaaaaaaa
     3661 tttatataat agtactaaat taagggtatc atatatggtg taatggtatt ttaatttaac
     3721 attattatca aattaaaata taaacaattg ttgtaatgtc tatatataaa gtatataatt
     3781 aaactaaaat gtaaaaattt acatattatt ttaaaaaaat acacgagtgt tttataaata
     3841 aaatgtaata aataattaat taacaaatat aaattttaaa ataagttaga tatttagaaa
     3901 aatcgaaatt tgtaggattt aaaggacata tacaaggagg agttaataat ggaaaaatta
     3961 caaggaaaaa ttgcagtagt tactgcagca acaaaaggta ttggattagc atcagcagag
     4021 atattagcaa aaaatggagc aactgtgtat ttagcagctc gttcagaaga attagctaat
     4081 gaagttataa ataaaataag tgcagaaggt ggttgtgcta agtttgttta ctttaatgct
     4141 cgtgaagaag agacttttac ctcaatgata gaggaggtag ttaaaaaaga aggtaagata
     4201 gatatacttg taaacaattt tggttcaaca aacccttctc ttgacaaaga ccttgtaagt
     4261 ggggatacag ataatttctt tgacacagta aatactaatt taaaaagtgt gtatttacca
     4321 tgtaaagcag caattcctca tatgataaag aatggaaaag gcagtatagt aaatatatca
     4381 agtataggtt cagtgttacc tgatttatct agaatagctt actgtgtatc aaaagcagca
     4441 atcaactcat taactcaaaa catagctaca caatatgcta aagacaatgt tagatgtaat
     4501 gctgtacttc cagggcttat tgcaactaag gcagcattag acaatatgtc tccagagttc
     4561 ataaaagaat ttttaaagca tgtcccttta aatcgtatag gagaaccaga tgatatagca
     4621 aaagctgttc tattctatgc tagtgatgac tcatcattta taacagggga tttacttgaa
     4681 gttgcaggag gatttggttt acctactcca caatttgcag ataatatatt aggataatta
     4741 aattaataat atgcaatgaa agcttatgta ttagttatag tataaaggat atttttataa
     4801 agaccataga tataatttat gttggaaata tttgaggttc aaaattccaa gttaaattta
     4861 tttatggtct ttattttgta tattttaata aattgtatat atttgtataa atgtaattat
     4921 ttacaatcat tttaatatgg aattataatt aaataggggg gatggtattt gtataaaatt
     4981 ttatatagaa aattagacct ataaatgaaa aatatggatt ttatattgga aaattgtaca
     5041 taatacataa tatatgctta aaataattta caaaaaatca aaaataatgc atagtttaca
     5101 tataagataa aaaatatatt gaaaaatact tgacattatg ctaaaattat tagatgcgtt
     5161 agaatgaact attgttttat ttttaattat tgagaggtga aaagtgaatg ccacatcctg
     5221 tcacgatagg taaaagaact agaatgagct tttctaaaat taaggaaata gctgatgtgc
     5281 caaatcttat agaaattcaa gtagattcct atgagtggtt tttaaaagaa ggtttaaaag
     5341 aagtgtttga tgatatttca ccaatagaag attatactgg taatcttata ttggaatttg
     5401 tcgactattc tctagatgat aagccaaagt acgatataga agagtgtaaa gagagagatg
     5461 ctacatattg tgcacctcta aaggttaagg taagacttat aaataaagaa acaggtgaga
     5521 taaaagaaca agaagtgttt atgggtgact tccctttaat gacagaaaga ggaactttcg
     5581 ttataaatgg agcagaaaga gttatagtaa gtcaattagt tagatctcct ggtgtttact
     5641 atgcggaaga aagagataaa acaggtaaga gattgatttc atctactgta attcctaata
     5701 gaggagcatg gttagaatat gaaacagatt ctaatgatgt aatatctgta agagttgata
     5761 gaacaagaaa acaaccagtt acagttttac ttagagcttt aggaatagga acagatgcag
     5821 agataattga ccttttagga gaagatgaga gattatctgc tacattagaa aaagataata
     5881 ctaagacagt agaagaaggt cttgtagaga tatacaaaaa attaagacca ggtgaacctc
     5941 ctactgtaga aagtgcatca tctttattaa atgctttatt ctttgaccca aaaagatatg
     6001 acctagcaaa agttggtaga tataaattca ataagaaact tgccttatgt tatagaataa
     6061 tgaataaaat ttcagcagaa gacataataa atccagaaac aggagaagta tttgtaaaag
     6121 ctggagaaaa aataagctat gatttggcta aagccataca aaatgcaggt ataaatgtag
     6181 taaatctact aatggatgat gataaaaaag ttagagttat aggaaataat tttgtggata
     6241 ttaagtctca cattgatttt gacatagatg atttaaacat aaaagaaaaa gtacattatc
     6301 caactttaaa agaaatttta gatggatata gtgatgaaga agaaataaaa gaggctataa
     6361 aatcaagaat taaagaactt ataccaaaac atatattatt agatgatata atagcttcaa
     6421 taagttatga gttcaatata ttctataata taggaaatat tgatgatata gatcacctag
     6481 gaaacagaag aataagatct gtaggtgaat tgttacaaaa tcaagttaga attggtcttt
     6541 caagaatgga aagagttata aaagaaagaa tgacggttca agatatggaa gctataacgc
     6601 ctcaagcgtt agttaacata agaccagttt cagctgcaat aaaagaattt tttggtagtt
     6661 ctcagttatc tcagttcatg gatcaaacaa atcctctatc tgagttaacg cataagagaa
     6721 aattatcagc ccttgggcct ggaggtcttt caagagaaag agctggattc gaagtgcgtg
     6781 acgttcacca ttcacactat ggtagaatgt gtccaataga gactccagag ggaccaaata
     6841 tcggtcttat aaactctcta ggaacttatg caaaaataaa tgaatttgga tttatagaat
     6901 caccatatag aaaatttgat aaagaaacat caacagttac tgatgaaata cattatttaa
     6961 ctgctgacga agaagattta ttcgtaagag ctcaggcaaa tgagccatta acagaagatg
     7021 gtaaattcgt aaaccataga gttgtttgta gaactgtaaa tggtgctgtt gaaatggttc
     7081 ctgaaagtag agtagattac atggatatat ctcctaagca ggttgtatct gttgccactg
     7141 ctatgatacc tttcttagaa aatgatgatg ccaaccgtgc ccttatggga gcgaacatgc
     7201 agcgtcaggc agtgcctcta gttagaagag aagcaccaat tataggaact ggtatagaat
     7261 acagagcagc aaaagactct ggagcagttg ttgttgctag aaattctggt atagcagaaa
     7321 gagtaacagc agatgaaata attataaaaa gagaagatgg aaacagagat agatataatc
     7381 tacttaagtt taaacgttca aactcaggta cttgtataaa tcaaacacct ataataaata
     7441 agggtgacca gataatgaaa ggtgatgtta tagcagatgg tccagcaact gatttaggag
     7501 aagttgcact tggaagaaac tgtcttatag cattcatgac ttgggaaggt tataactacg
     7561 aggatgccat attaataaat gaaagattag ttaaagaaga tagattatca acaattcata
     7621 tagaagaata tgaatgtgaa gctagagata caaaactagg accagaggaa ataactagag
     7681 atatacctaa tgttggagat agtgcaatta agaacttaga tgacagaggt ataataagaa
     7741 taggtgcaga agtagactca ggtgatatat tagttggtaa agtaactcca aaaggagaaa
     7801 ctgaacttac tgctgaggaa aggttacttc gtgcaatatt tggagaaaag gctagagaag
     7861 ttagagatac ttcacttaaa gtgcctcatg gtgaatctgg tataatagtt gatgtaaaag
     7921 tatttactag agaaaatgga gatgatttat ctccaggagt aaatgaatta gtaagatgct
     7981 atatagctaa aaagagaaaa ataaaagttg gagataaaat ggctggtcgt catggtaata
     8041 aaggggttat ctcaagagta ttacctgaag aagatatgcc attcatggaa aatggaacac
     8101 cactagacat aatacttaac ccacaaggta taccatcacg tatgaacatt ggtcaggttt
     8161 tagaagttca tttaggactt gctgctaaga cattaggatg gtatgtagca acatcagtat
     8221 tcgatggtgc taatgagtat gacataatgg atgctcttga agaagctggg tatcctagag
     8281 atggtaaatt aactttatat gatggtagaa caggtgagtc ttttgacaac agaataactg
     8341 ttggatatat gtactacttg aaactacatc acttagtaga tgaaaaacta catgcaagaa
     8401 gtactggacc atactcatta gttactcaac agccactagg tggtaaagca caatttggtg
     8461 gacaaagatt tggagagatg gaggtttggg ctctggaagc atatggagct gctcacatac
     8521 ttcaagaaat acttactgtt aagtctgatg acgttgtagg tcgtgttaga acatatgaag
     8581 ctatagttaa aggtgaaaat atacctgaac caggaatccc agaatcattt aaggttctta
     8641 taaaagaact tcaaagtttg tgcttagatg taaaagtatt gacagatgaa gaccaagaaa
     8701 tagaggtaag agaatctgtg gatgaagatg atacaatagg tgaatttgag ctagatgttg
     8761 taaatcatat gggagaagtt gaagaaagta acataataga agaaattgaa gatgattttg
     8821 cagaaaatgc agaagatgag gatatagaga atttagaaga attcactgaa gatgatttat
     8881 ttgaagaaga aatagatttt gatagtgatg actttgatat gtaaatttaa ttaatgtaaa
     8941 tctcaaatag aagggagaga actccttgtt tgaattaaac aatttcgagt cgataaaaat
     9001 agcattggct tctccagaaa aaataagaca atggtctagg ggagaagtta aaaagccaga
     9061 aactataaat taccgtactt taaaaccaga aaaagatggt cttttctgtg aaagaatatt
     9121 tggaccacaa aaagactggg agtgccactg tggtaaatat agaagagtta gatataaagg
     9181 tgtagtttgt gatagatgtg gagtagaagt aactaaatca aaagtaagaa gagagagaat
     9241 gggacatata gagctagctg ctcctatgtc tcacatctgg tacttcaaag gtataccaag
     9301 tagaatggga cttttacttg atatgtcacc aagatcgtta gaaaaaatat tatactttgc
     9361 ctcatatgta gtagttgatc caggagaaac tggattaaac gaaaaacaat tgcttacaga
     9421 aaaagaatac agaactgctc ttgaaaagta tgggtatact tttactgtag gaatgggtgc
     9481 tgaagctgta aagacattac tacaaaatat agatttagag caacaaagta aagacctaag
     9541 agcggagtta aaagatagta cagggcaaaa gaaagttaga acaataagaa gattagaagt
     9601 tgtagaggca tttaaaaagt ctggaaataa accagaatgg atgattttag atgcaatacc
     9661 cgtaatacca ccagatttaa gaccaatggt acaacttgat ggtggaagat ttgcgacttc
     9721 agacctaaat gacttatata gaagagttat aaatagaaac aatagactta aaagattatt
     9781 agagcttgga gctccagata taattgtaag aaacgaaaaa agaatgcttc aagaagcagt
     9841 tgatgcgtta atagataatg gtagaagagg tagacctgta acaggtcctg gaaatagacc
     9901 acttaaatct ttatcagata tgctaaaagg taaacaaggt cgtttccgtc aaaacttact
     9961 tggtaagcgt gttgactact caggacgttc tgttatagtt gttggaccag agcttaagtt
    10021 ctatcaatgt ggacttccaa agaagatggc attagagcta ttcaagccat ttgttatgga
    10081 taaattagtt aaagaaggat atgcacataa tataaaaagt gccaaatcta tagtagaaaa
    10141 agttaagcca gaagtttggg atgttttaga agatgttata aaaagccatc cagttcttct
    10201 taaccgtgcg ccgactctgc atagattagg tatacaagca tttgaaccta tcttagttga
    10261 aggtaaagct ataaagctac atcctcttgt atgtacagct tacaacgcag actttgatgg
    10321 tgaccaaatg gcagtccatg tacctttatc agtagaagcg caagcagaag caagattctt
    10381 aatgctttct gtaaataata tacttgctcc taaagatggt tcacctataa ctactccatc
    10441 tcaggatatg gttttaggtt gttattatct aacaatagaa gcacaagatg gagctaaagg
    10501 aacaggtatg gtatttaaag actttaatga actattactt gcttattaca ataagtcagt
    10561 tcatctacat gcattagtaa aactaaaggt aacacttgaa gatggaagaa gttcattggt
    10621 tgaaagtact gttggtagat ttatatttaa tgaaaatata cctcaagact taggttttgt
    10681 agatagaaaa gaaaacccat ttgctcttga agttgatttc ttagcagata aaaagtctct
    10741 tggtaaaata atagataaat gctttagaaa acatgggaat acagaaacag ctgaattact
    10801 agattatata aaagctctag gatttaaata ttctacatta ggtggtataa cagttgctgt
    10861 tgatgatatg agtgtcccag aagaaaagaa agtatttata gctgaggcag aagctaaagt
    10921 tgataaatat gaaaaagcat acagaagagg tctaatctct gatgaagaaa gatatgaaaa
    10981 agttatagag acatggacag aaacaactga taaagttact gatgctctta tgggtggact
    11041 agatagatta aataatatat atataatggc acattcagga gccagaggtt ctaaaaacca
    11101 aattagacag ctagcaggta tgcgtggtct tatggcaaat gcatctggta aaacagttga
    11161 gataccagtt aaatctaatt tccgtgaagg tttatcagta ctagaatact ttacatcttc
    11221 acatggtgcc agaaaaggtc ttgccgatac agctatacgt acagctgaat ctggatattt
    11281 aacaagaaga cttgttgatg taagtcaaga tgttattgta agggaaatag attgtggaac
    11341 agaagatact acagagattt atgctataaa agaaggaaat gaagttatag aagagatata
    11401 tgacagaatt gtaggaagat atactataga ccctatatta aatcctgaaa ctggagaagt
    11461 tatagttgaa gctgattcaa tgatacaaga agatgaagca gaaactatag tagctttagg
    11521 aattgaaaaa attaggataa gaacagttct taactgtaaa actaatcatg gagtttgttc
    11581 taagtgttat ggtagaaact tggcaacagg aaaagaagtt aatataggtg aagcagttgg
    11641 tataatagca gctcaatcta tcggtgagcc gggtactcag cttacaatgc gtacattcca
    11701 tactggagga gttgcaggag ctgatataac tcaaggtcta ccaagggttg aagaattatt
    11761 tgaagctaga aaaccaaaag gattagctgt aataactgaa gtatctggta gagttgaaat
    11821 tgatgaaaca ggaaagagaa aagaagtaaa tgtaatacca gaagaaggcg agactcaaac
    11881 atatgtaatt ccatatggtt ctagattaaa agttaagcaa ggtcaaatgc tagaggctgg
    11941 agaccctcta acacaaggat ttataaatcc tcatgatatc gtaagagtaa atggtgttaa
    12001 gggagttcaa gaatatatag ttaaagaagt tcaaagagtg tatagacttc aaggggttga
    12061 tgttaatgat aagcatatag aagttatagt aagacaaatg ctatctaaag taaaagttga
    12121 agacccagga gatacagatt tattgccagg tggatatgaa gatgtattaa cattcaatga
    12181 atgtaataaa gatgctatag ataaaggtct aagaccagca gttgctaaaa gagttttact
    12241 tggtataact aaagcatctc ttgcaactga ttcattctta tcagcagctt ctttccaaga
    12301 aacaacaaga gtattaacag aggcagcaat aaaaggtaaa gaagaccact taataggact
    12361 taaagaaaat gttatattag gtaagttaat accagcagga acaggaatga agaaatatag
    12421 aaatatagct gttgaaaaaa ttgaagatta atagcatata gagtaaagaa atcttgacaa
    12481 ccattatttg tggtgttaaa atagtaaagt gtgttgttta aaagtacact aagggcttga
    12541 acaagctctt agtgactttt agatggcaat aaataagaac taaaaagcat caagctagaa
    12601 aagtgctaat atcacatatt tatgtgttat agacattaaa ttgtaagtaa cgaatgttac
    12661 aatcagtcag tatgaatatt catattgatg tagttggcgg tcactgataa gatcgaaaat
    12721 ttggaaggag gtgcagatga tgccaacaat taaccaatta gttcgtaaaa gtagaaaagc
    12781 gctagagaag aaatctactg ctccagcatt acaaaaaggt tacaactctt taaataagaa
    12841 agtaactgac gctagtgccc cacaaaaaag aggggtttgt acttcagtaa aaacagttac
    12901 tcctaagaag cctaactcag ctttaagaaa agttgccaga gttagattaa ctaatggtat
    12961 agaagtttct gcttatatac caggagaagg acacaactta caagagcata gtgttgttct
    13021 tataagagga ggaagagtaa aagaccttcc aggggttaga taccatatac taagaggtac
    13081 tctagataca gcaggtgttg ataagagaag acaatcaaga tctaaatacg gtgcgaagag
    13141 acctaaagaa gctaaaaaat aaaacaatta atgattgatt atagttcggt gctttaaata
    13201 tatatatatt tacggcatca cggcattaaa tgatttatgt cgagtaccta tgatttaagt
    13261 taaataatta aggagggaag cgaaatgcca agaaaaggta atattccaaa aagagaagtt
    13321 ttaccagatc ctatgtatgg gagtaaggta gttactaaat taataaataa tctaatggta
    13381 gacggaaaaa aaggaaagtc tcaaagaata gtttatgatg ctttcgcaat tgttgctgaa
    13441 aagacaggag aagaagcttt agaagtattt aataaagcaa tggataatat aatgcctgtt
    13501 ttagaagtaa aagcaagaag agttggtgga gcaaactacc aagtgccaat cgaagttaga
    13561 cctgaaagaa gacaaacatt aggtttaaga tggctagtaa aatatactag agctcgtgga
    13621 gaaaaaggaa tggttgaaaa gctagctaaa gagataatgg acgcagctaa caacactgga
    13681 gcttcagtta agaaaaaaga agatactcat aaaatggcag aagctaataa agcatttgct
    13741 cattacagat ggtaggatat atagatatct tataaattca aggatatcat aaggaggata
    13801 accatggcta gaaagtttcc tttggaaaaa actaggaata taggaatcat ggctcatata
    13861 gatgcaggaa agactactac tacagaaaga atcctgtttt atactgggca aactcataaa
    13921 ataggagaaa ctcatgaagg agcttctcaa atggactgga tggagcaaga aaaggaaaga
    13981 ggtataacaa taacttctgc cgctactact gcttcatgga aagaccatag aataaacata
    14041 atagatactc ctggacacgt ggacttcaca gttgaggttg aaagatctct aagagttctt
    14101 gatggttcag ttgctgtatt ctgtgcaaaa ggtggggttg agcctcaatc tgaaaatgta
    14161 tggagacaag ctgaaactta tggagtacct agaatagctt ttataaacaa aatggatata
    14221 ttaggtgcag atttctacaa tgttataagt atgatgaaat ctagattaaa ttctaatgca
    14281 gttcctatgc aattaccaat aggtaaagaa gattcattta taggtataat agacttatta
    14341 aaaatggatg ctgttatata caaagacgac ctaggggttg aaatggaaga aacagatata
    14401 ccagaagata tgaaagaatt agctgctgag tggagagaaa aattagttga atcagtagct
    14461 gaaactgatg aagaattaat gatgaaatac cttgaaggtg aagaacttac tatagatgaa
    14521 ttaaaagttg ctataagaaa agctactata gcttgtgaaa tgaacccagt tttctgtggt
    14581 acagcttata gaaacaaagg tgttcagtta gtaatagatg cagttttaga ttacttacca
    14641 gcaccaactg atataccagc tataaaaggt atattagcag atggagaaga agctgaaaga
    14701 cattcttctg atgaagagcc attctctgca ttagcattca aaataatgac tgacccattc
    14761 gttggaaaac tagcattctt cagagtttat tctggaacgt tagaatctgg ttcttatgtt
    14821 ttaaatgcaa ctaagaataa gagagaaaga attggtcgta tattacaaat gcatgccaat
    14881 acaagagaag aaataactaa agtttatgct ggagatatag cagcagcagt agggttgaaa
    14941 gatactacta ctggagacac attatgtgac cctgctaatc caataatact tgaatctatg
    15001 gaatttcctg agccagttat atctgttgct atagagccaa gctctaaagc agctcaagaa
    15061 aagatgggta tagctcttca aaaattagct gaagaagacc caactttcac agttaagact
    15121 gaccaggaaa caggacaaac tataatatct ggtatgggtg aattacacct agagataata
    15181 gtagatagat tattaagaga atttaaagta gaagctaaag ttggtgcacc acaagttgca
    15241 tatagagaga ctataactca accagttgat gttgaataca agtactctaa gcaatcaggt
    15301 ggtagaggac aatacggtca cgttaagatt agagttgctc ctcaagagcc aggtgaagga
    15361 tacaagttca caaacaaaac tgttggtgga tcagtaccaa aagaatatgt tggtccagtt
    15421 gacatgggta tacaaggagc tatgcaatct ggtatagttg ctggatatcc agttgtagac
    15481 gttgctgttg agttatatga tggttcttac catgaggttg actcttctga gatggcgttt
    15541 aagatggctg gttcaatggc attcaaagat gctatgaaga aaggtaatgc agttctttta
    15601 gagccttact tcaaagtaga agttgttact ccagaagatt acatgggaga tgttatgggt
    15661 gacttaaact caagaagagg attaatccaa ggtatggaag ctagaagtgg tgcacaagtt
    15721 ataaatgcat tcgttccact ttcagaaatg tttggatact caacagacct aagatcttca
    15781 actcaaggtc gtgcaactta cacaatgata ttcgatcact atgagcaagt tccagcttca
    15841 gttgctaaga aaatagctga aggaaaataa tttaaaaata attaataata taaatataaa
    15901 aaataatcag ctagatgaga tatatctcat ctagcacaag at
//
LOCUS       DAEMAE010000080        15729 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEMAE010000080 DAEMAE010000000
VERSION     DAEMAE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.082"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..997)
                     /locus_tag="KPH93_001955"
     CDS             complement(<1..997)
                     /locus_tag="KPH93_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG1688775.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKGFELVAVYSRNLEKARE
                     FGTPHGASVFFDDLEQMAKSKDIDAVYIASPNALHSRQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFRAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPIVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKIADSYIPSEIQGEDGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGIYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSI"
     gene            complement(1174..1713)
                     /locus_tag="KPH93_001956"
     CDS             complement(1174..1713)
                     /locus_tag="KPH93_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG1688776.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(1814..2494)
                     /locus_tag="KPH93_001957"
     CDS             complement(1814..2494)
                     /locus_tag="KPH93_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG1688777.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTNIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDCFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2523..3812)
                     /locus_tag="KPH93_001958"
     CDS             complement(2523..3812)
                     /locus_tag="KPH93_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG1688778.1"
                     /translation="MRELLKVTLIVMLVLVNAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGVGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(4262..5863)
                     /locus_tag="KPH93_001959"
     CDS             complement(4262..5863)
                     /locus_tag="KPH93_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1688779.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINGNAN
                     KDIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     IYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRALILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKDGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIINGKIALENGEITENKCGEVLKR"
     gene            complement(5974..6651)
                     /locus_tag="KPH93_001960"
     CDS             complement(5974..6651)
                     /locus_tag="KPH93_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688780.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(6664..7359)
                     /locus_tag="KPH93_001961"
     CDS             complement(6664..7359)
                     /locus_tag="KPH93_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG1688781.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(7411..8610)
                     /locus_tag="KPH93_001962"
     CDS             complement(7411..8610)
                     /locus_tag="KPH93_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG1688782.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDVQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(8871..10916)
                     /locus_tag="KPH93_001963"
     CDS             complement(8871..10916)
                     /locus_tag="KPH93_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688783.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIIHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKFQSKGFVSKYTFESVVG
                     ESKIIKEKINIAKKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKFKGVI"
     gene            complement(11328..12137)
                     /locus_tag="KPH93_001964"
     CDS             complement(11328..12137)
                     /locus_tag="KPH93_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1688784.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LKYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSVLYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(12221..12754)
                     /locus_tag="KPH93_001965"
     CDS             complement(12221..12754)
                     /locus_tag="KPH93_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1688785.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGIS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(12905..14254)
                     /locus_tag="KPH93_001966"
     CDS             complement(12905..14254)
                     /locus_tag="KPH93_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG1688786.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVNTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVSNGTLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFNLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(14266..15648)
                     /locus_tag="KPH93_001967"
     CDS             complement(14266..15648)
                     /locus_tag="KPH93_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG1688787.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPAQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIDAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDVLMNIDMVGDNLKQAQGMCGSSSGSIPTNVGQPMI
                     RVREITVGGR"
BASE COUNT         5065 a         2938 c         1536 g         6190 t
ORIGIN      
        1 caatactact ttgtcttctt atctcatcca taatttccat tactattatt gaattttcat
       61 gagaatttat atttgattct attttattgt tatttataag acttataaac tctacaagtt
      121 cataatatat tccttctatt tctctaggct tttcctctgc tttttctttt ggaatagata
      181 tatcttcttc tcttccatct ctgtatttaa taatagcttt ttcaaataga ttaagctttt
      241 caattattat acttccatct tctccttgta tttcagatgg tatataagaa tcagctattt
      301 tagaatactg aattatcgca tccatatcat catattgaaa tacagctgaa ccttctccat
      361 ccactccagt ttgaagtata tgagaaactg cctttacagt ttttggagca ccaaataaat
      421 tcactatagg atgtatacaa tacactccta tatccattaa agctccattt gacaactctt
      481 ttttaaaggc attttctata attccatttt tatatttatc atatcttgaa gaatactgac
      541 aatagcttcc aaaaaatctt cttattttac ctattttata caagttttct ttaactgctc
      601 taaagtttgg aacacatgta attctcatag cttccataag caatacatta ttatctttgg
      661 ctgtttttat cattgatttg acttctttta aattagatgc taacgacttt tcacaaagca
      721 catgtttttt gtgttttaaa caagtaatgg cttgccttga atgtaatgca tttggagaag
      781 ctatatacac agcatctata tccttcgatt tagccatttg ttctaaatca tcaaaaaaaa
      841 cacttgctcc atgtggagtt ccaaattctc tagctttttc taaatttcta gaatatacag
      901 caaccaattc aaagccttta accctagatg cagctcttag aaatatattt gctatgttgc
      961 ttgtcccaac aataccaaat cttattttac ccatcataaa tcactcctcc attttctaca
     1021 ctttatttta attttaataa aaaactttac aacttataga tatattataa aataatttta
     1081 tcaaataaaa aagaaagcta attttaaatg attttaccat tttaacatag ctttctttta
     1141 actacaatct aaattcaact tatattttta aagttatctt tctactatat ctttatttcc
     1201 cctcaaaaat tttatagtaa gaatttcatc cacaaacaat gttactaaaa gtatagttac
     1261 aacaattggc caataaggta ttataagact atataaacta tcaactatat acacttgaaa
     1321 atatctaact ccaataaatg tcaatatagt ccaatataca gaaaacttta gacatactat
     1381 tcctctacta ttgtacttat aatctgaata atcccaatag accttattaa aatatacttt
     1441 cataagtatt cctgtaatat attctactac tgtcggcact actagacata ataaaagcaa
     1501 tgcaaatata ttatttgggt caataaatct tgagacctct attataatcg acattgcaat
     1561 tgcatacatt ggtttaaatg gaccatttaa aaatccatct tcttgaaaat gcccttgttt
     1621 ataataacta tataagtttt caattatcca acctataaaa ccatacaaaa aaaaattaaa
     1681 taaaaaatat aatagagaac ctacattatc catgaatatt cctcctagct gtttatactt
     1741 attttttctc atattcatgg atttattcta aatcaaagaa attatattta atttatttaa
     1801 ttaatatttt atattataga ttgtgacatt ctttagagtt taaacttttt aggtattctc
     1861 taacttccaa taaattttcg agtatcttat gtgattgtga tttaatttgc tcatctggct
     1921 cttttaaatc tggtacattt atacatctta taccaccatt gtatgcagct tcgactccca
     1981 taggtgaatc ctctatgaca atacagtttt tgggattaac atttatcttt ttagctgctt
     2041 ttaagaatat ctcagggttt ggtttagagt ttacaacatc atctccacaa actatagcat
     2101 caaagcaatc ttttaaattt gccttagcta atctctttac tgctctttct ctctttgttg
     2161 atgttgcaac agccatcttg tatccatttt cctttaaaaa agagattagt tcattgactc
     2221 ctaatttgat aggagctccc ttcctctcca taaattcaat catattttta gttttttcgt
     2281 catataaatc tattattggc acagaactat cgtatatatt tgtaagacct tctattattc
     2341 ctttacgatt tcttcccata actgatgtat aaatttcctt agtcatagta tatccatact
     2401 tttcaaacgt ctccatccaa aattcaagag aaattctctc tgagtcaaac aaaactccat
     2461 ccatgtcaaa aataattcct tctacttttt gcatacaatt cctccaatat tttttgatat
     2521 aattatatta cactttttat gattaatttt acaaccctca atttatccaa ttctaatatt
     2581 gtaaaattgt aattttcaaa agtgacgctt tggtttactt taatatttga accaagcata
     2641 taggatatcc acccacctat actgtcaaat ccatcatgct ctatatcaag tccaaaatac
     2701 ttattaatat agttaataga ttcagttcca tcaactaagt aagaacctac tcctattttc
     2761 ttaatatgag tctcttcttt gtcaaattca tcttgaattt ctccaactat ctcttctagt
     2821 atatcttcta ttgttaccac accagatgta ccaccatatt catccacaac tatagcaagt
     2881 tgaagttttt cctttcttat tctctcaaga gctttatcta tagttaaatt ttcagatata
     2941 tatataatat ccctcaatat ttcttctagt tcaattttat tttcatttat cttttgattg
     3001 tataaatctc ttatatgtac aaaacctaat atatcatctt tattttttct gcatactgga
     3061 tatcttgtaa caccttcctc ttttaaaata gctaatatct tttcttcaga gtcactttca
     3121 tatatacata ccatatcagt tctaggaacc attatctctc taattttctt ttcttcaaat
     3181 tcaaaaatat tatcaactaa tctttgctct gattcatcta taagtccact tttataactt
     3241 tcttctacta atagtcttat ttcgtcacct gtatggggtt catcaacctc atcagcttgt
     3301 gagtatccaa atggtttaag aaataaatca gtacttaagt tgaaaaaata tattattgga
     3361 tatgttaact tataaaaccc tactaataaa aaagatgtac tcaacattat tttttctgta
     3421 ttataaagtg ctaaagcttt aggaaccaac tctcctatta caacctccat cattgttata
     3481 attaaaaaag aaatggaaat tgatatagta tatataatac tttcactaag attaaataca
     3541 cttactattg gtaaaatcaa ctctttaact gtagattctc ccatccaacc taatgcaagt
     3601 gaacaaagcg ttataccaag ttggcatgca gaaagatatg aatttaaatt gtcttttatt
     3661 attttacatc tttttgcatt attattacct tcagttatca tggtatcaat cctagaacta
     3721 cgaagtttta ccatagcaaa ttcacaagct acaaaaaaag catttactaa aacaagcatt
     3781 actataagtg ttacttttaa taactcccgc ataaagtatt ggttcttata atgtaagaaa
     3841 tctataagaa ctcaaagttc cccccttata ttaataaatt taatttataa tatatattct
     3901 atcatattta ttaatatatc ctactttatt acaaataatt tttatgaact taataacccc
     3961 ttttaatatt tatgattggc tatttgataa atattaaagg ggttgtatag ttatcatttt
     4021 ttatttctag aaaatctttt ttcaaatgat ttagttatct atttatccaa tcttttacat
     4081 aatttagtga ttaaaaatat taattttttc actataaagt ataaatatta tctgattatt
     4141 aaaataaata ttaatactat aaataaaaac gaagcatgat ttttatttaa ataaatctca
     4201 aacttcgtct ttacttactt agatatttat ttactttcta aatcctatta tttttaaaac
     4261 cttatctttt taatacctca ccacatttat tctcagtaat ctctccattt tctaacgcaa
     4321 tttttccatt tataattaca tattcaacac cattggctag taattgtggc tttgaaaaat
     4381 caatatcttc tgacctagga taactatagt tatccatgtc aataactgct atatcagcta
     4441 tcaacccgtc tttaagaata cctctatttt ttattttaaa ctgcctagct ggtaatgaag
     4501 taactcttct tacaatctct tccaatctaa caccagactt tctgtacata ttgaagaaaa
     4561 ttggaaaagc tcctcttctt tgtagttcaa accatgacat atccccacct acactaaaca
     4621 aacaatctga accaaccata acaaatggat tatcaattat tttattatca tgtagtttat
     4681 ctgggaaatc agctctatac attcctccta aacaaaagat aagttcttcg tctccatcta
     4741 aaagtaaatc gagtaataac ttgtcctcat ctattccttt ttgtgtagct atttcacgta
     4801 ttgatttctt ttccatatta atatctttag tgtttagaag tattatactt cccctatctc
     4861 caagtataaa tgcttctttt aatataagtg ctcttccctc ttcatttcta agagcatcca
     4921 ttaccccatc tatacctaat tcaaatagac tagatgatat tgccataata aattgaatta
     4981 ctcttttttt cttcatacaa tgcccagaac ttcttggtac tgtgtcaacc attatttcaa
     5041 caccattata cctagccttt aatatctcat caaatgcttc tatttcaact ggacttaagt
     5101 gagatacttg aaccctagct ccactttttt gcccaacctc tatggcttct tttactgcat
     5161 tatatcttcc tatgtaatct ctaatatgag atgtatatat accatcatgc tctttaatct
     5221 gccttgcaat atcaacaagt tcatcaatat cagcatactt acttggtata tatgccaatc
     5281 cagtagatat tccaaaagca ccacttttca ttccatcatt tattatatct gtaatttcat
     5341 ttttttcctt ttctgtagga ggtctatcct tagaacctcc cataacactc catcttatag
     5401 tcccatgccc caacaagaac cccatattga cagaaatacc acttttagat ataaggtcac
     5461 aatattctgt aaaattattc caacatttat ttttatctat aagatacttt ttttcttctt
     5521 caagtaatag gccattttta tacatatatt catatatatt ttcagaagaa tatggtgtta
     5581 tagaatgtcc acaattacca tttatagttg ttgtaactcc ttgttttaga aacttttcaa
     5641 atttaccatc tagtattgct actatttctt catgaacatg agggtcaata aatccaggtg
     5701 ttataacttt tccacaacaa tcaatatctt tatttgcatt gccatttatc ttagaatcta
     5761 tttttgctat taagttgtct ttaattgcta tatctccata aaaaccagga ttacctgtac
     5821 catctattat aaatccattt tttaagatta aatcgtatat caaaatatcc cctccttaaa
     5881 aaccaatttt taaataatct taatgtaatt tttatatttg aaaatcaaat tattatattt
     5941 catatttgaa aattcactat tacttcattg taattaatca ctaagcttat ctttatacat
     6001 agctcttgca attaatatag taccaaatac acatataaga actactagag actgtggtat
     6061 tataccttgt tttgataata taatagttaa tacagatatt acaattgcta ctagacctaa
     6121 ttttttatct tgtaaaaata cttgtccaaa aactgaccca aatagagctg gtaaaattaa
     6181 gtttaatttt tctactactt cagctggaat tttagataat acacttcctc ctaaaattac
     6241 gcctatagtt agtatactta tattcactaa aatagataca gctattccta ttgtagatat
     6301 tatcgagcct tcttctgtac ctgattcaac ttctgctgcc ttttgagctg ctattgaaca
     6361 tggtaacctc atattagata tattaccaga aagaaaactc atataagttc ctgggatacc
     6421 taagattggt gaataagaaa ctggttctat tatgtaattt ggagcactca tactgacaat
     6481 tgccattgta cttgctaata atgctgatat tggaggcata ataccaaatc caaaactcaa
     6541 aattattcca gggactaatg ctgctataat acccaaagat agtgttaatc ttccatacct
     6601 tatcataaaa ggtaaatatt cttcattata tatagatttt actttactca taaaactccc
     6661 cccttatatt actgaagcta ttatcattcc gctaaacatt gatattgtaa gcccccactc
     6721 tcttagccat tttatatttt tcttcttttc aagcatacac aatattacca ttatagccat
     6781 tccacttata catgcaattg ttccattatc aaagcgcaac accctatcgg catttagata
     6841 tgcaaatgaa cccaacattg ctcctagaga tattattggt ataaatgatt tcttgccact
     6901 agatataaca ttggttatct tatctagttt atgtccaaat agtaatgtga atattatcca
     6961 tcctaaagaa cctaatatca tagtccaaac tgcatttgta aatgcaagtt ttgtcatttc
     7021 aggagaacct aaagttatac ctaatgcatc tgttccaaat ccggctgaca ttgattcaaa
     7081 aattacagaa cctataaatg ataacctcat ccatgctata ggcccaccca tagttactat
     7141 aagcgatacc attccagcaa atatagtcat tgaaggccca atagctgaaa tagcactact
     7201 cttaacagct gactttattt gtttatctgt tattcccatt gttttaccaa ctttgcaaga
     7261 ttttatagca aatataaggg attgtaccac tactattgat actgctactg tactcgctat
     7321 ccacaaaagt ggatgattag ctaattttaa ataatccatt tataaaacct ccctataaat
     7381 cctcaaattt tattaatatt tctaatatat ttatttccaa acctgtttta atactctaag
     7441 tatatttcca cctaatacct tctctatttc ctcatcacta tatccatgct ttactaaata
     7501 tctgacaatg ttttttgaag tttctgttgg attctcaaga ccttttacat attctacttt
     7561 gggatgttct tttgtacctg ttattttttt tatatcaaat ttagcagcta gtactgagtg
     7621 taattttaca tggtcgccat atactgtatc tggtccaaaa gctacatggt ctattcctac
     7681 taaattttta atatactcaa aatgttccat aaatgactct attgaatgtt ctttattctt
     7741 ctctgttatt gtagtatgag gtgctgcttc tacacctata acccctcctt ttttagcaca
     7801 tgctattaat acttcatctg gagttaatct ctttgtgttc cataaacttc ttgccccaac
     7861 atggcttaat attattgggt ccttgctata ttctattaca tcaagtgtag tttggactcc
     7921 aacgtgagaa caatctattg ccattccaat tttattcatt ctttcaacag cttttttccc
     7981 aaaatttgta agacctccat ctttttcttc ttttaatcct gaacctaatg cattggattc
     8041 actgtacgtt atacccatta accttatacc aaatccatac agtatatcta ttctatcaag
     8101 ttcattttct attggtgctg caccttctaa tgtcggtatc aatgctactt taccttcact
     8161 tttagctctg tatatatctt ctactttttc acactttatt acaaaatctt gatgagctaa
     8221 atcacatagt ctcatgccta aatctattaa tatatcatcc catttccacc ctgaatttga
     8281 tgttatcgta catacaccat ccattaaatt atcaaatata gcatcataat atcctcttga
     8341 taatgcttca taagcacatc tttgtttacc ttccctattg tattcaaata tatctctttc
     8401 taagtgttct ggaaataata ctggatgttc atgtaatgat ataaatattt tttcatccac
     8461 tatactttta actttagctt cttgaacatc atctaattca ataaggtact ctttaactct
     8521 tttatctcct ttacacatct caatagatga aatatctttt cctttttcta aataactata
     8581 ggcttcatat cctttataag ctgacttcat aacaacatcc tccaagttct acaatttttt
     8641 atatttttcc tatatagcaa ttgatatgcc aaaatataaa ttttaattgt ataataaatc
     8701 ttgcaaaaaa taaaaacagt agtaaggttg atatttacta ctgttttatt aaaaataatc
     8761 tttttagatt atttattcaa tatttttgaa ttatcttaat cttaaagata attttattta
     8821 tttctatcta ttatagggat ttttttaggg tgttttgcct ttttagtccc ctatatcact
     8881 cccttaaatt ttacatatga tataaagttc tttccttcat cagttataaa actaccttgt
     8941 ttttttactc cagataaaag taaaccatgg ctctttaact tatctattct ttttctaatt
     9001 tggtctacac ttaaaactat atctttttct tttaaaattt cttgcagttt atttcttcct
     9061 aataatatat ttttattatt ccaagtttct atactggtca atatacatat actttcctca
     9121 aagaaattcg attgtttgaa atccaggata attgaatcga aattttcatt aactaaagta
     9181 ttgtttttct caaatctaaa ttgttctggc aaatgctcgt aatctactct atcttcctca
     9241 actatagtat caatataata aattaaattt ttaagctctc ttacatttcc tggccattca
     9301 tacaatgtta aagcttgcat tgattcctct gtaaaacatt tgttgctatt tatttcatct
     9361 aaaaaatact tacttataag aggtatatct tccttacgtt ctctaagtct aggtatctct
     9421 atatgtaata cattaatcct ataatacaaa tcttctctaa aagaaccttc tttaattttt
     9481 ttctttaaat ctttatttgt tgcagctata attctaacat ctattggtat aattttactt
     9541 ccgcccattc tcataacttc tttttcctgt aatactctta gcaatctctg ctgtacatct
     9601 aaagatatat cacctatctc atctaaaaaa attgttcctg tatgtgctct ttcaaatata
     9661 cccatttttc caccttttat tgctcctgta aaacttcctt catcatagcc aaaaagttca
     9721 ctttcaataa gtgtatctga caaagaagat agattaactg caacgaatgg cttgtttttt
     9781 cttagagatt cattgtgtat tgcctgggca aatatttctt ttcctgttcc attttctcca
     9841 agtatcaaca cagaaaagtc tgtagttgct attttttttg caatattaat tttttctttt
     9901 attattttac tttcacctac aacactctca aatgtatact ttgatacaaa accttttgac
     9961 tgaaatttat tttgaatctt gtcctcaagc acttgaattg cacttatatt tttaatgctt
    10021 ataatactct ttattcgttc attattttca taaacattta ctttattaat tatcagtttt
    10081 ttgttattta aatttactac ttcatcatta acttcatctt cttgaaaaaa tatttttttg
    10141 acaaccttat cactaaataa gtccataaag ttattagata taatttcatt ttggtctatt
    10201 ccaactagat tagagaatac tttgttgcaa tatataaatt ttcctaattt atcaacagaa
    10261 gcaatacctt catctataat ctccaaaaaa ctcttcatgg ttttattcat agttgtataa
    10321 tatctatatc cacttactgt atcttcattg tatttttcct taataatatg taatttatct
    10381 atagatatat ttagttttgt aaagatttct ataactgtat ttatatcaat taccttatca
    10441 cctatgtcaa ttatttgctt tatgttttgt ggtatactgt ttctgcttcc cgtaataatt
    10501 ccaatctcac atttagactt atcacaacct ggataatatg gtattagatt aatatgaatt
    10561 aatcctagtt ttctaattat cttggcagtt tcatctgctg actctttata agcatctata
    10621 accataacat cagaatcatt ttctatactt attatctgat ttatattttc aatatttata
    10681 gtacgatgta ttattacaat aggtatattt ttatcaatat tattatgtat atattccttt
    10741 atatcattgt ctgaacatac tacaagctca tagcttgaca gttcaatatt tatcctattt
    10801 acatatacca agttatctat attacaaaat tctccaaata tattttccaa ttgttttgat
    10861 atatctatat ttattttgtc atcagttgat acaaataaaa tattatgttt tttcatagtc
    10921 ccacctcctg tttaacgagt atagaaagta ataatttggt ctattgtagc actttttaaa
    10981 aaagttttaa atcttaaaaa tcaaattttg taccatttac tattattttc cacaataaaa
    11041 taatgtaagc tatctttttt cacatacaat ttagtttcta aaacaattat tttgacatta
    11101 ttggaataga aataaaaatt taatataata gtgttaatat attaacacta ttttcaagaa
    11161 ttataaggaa taaattgtaa caaactttag gaatatataa taatagggtc tcagaactta
    11221 attacaagtt ctaaaaccct atttgtttag tgtaaaaaat atcataaaat ttagcttaaa
    11281 tcatttcata taatattact ctttttctaa gtgataataa aatatatcta ttcctctaaa
    11341 aactgtaaaa attcctgatt aaatagttta agctcatcat agaatatagc atgaccacta
    11401 tactcaaaag tatataaaac tgaatcttct attttttcat tcataaaaac tgcaaattca
    11461 tacggacata tttcatctaa tttcccatga aatataccag taggcactct tacacatttt
    11521 aaatcattaa ataattcttc atctcttaaa gatacagcag ttccaatagt gccaatacct
    11581 gatgcactaa aaccaatatc attaaaccat cttctaaagc tttctggtgg atttgatgca
    11641 aatacttttt cgccaaaatc cgttaccatt tctggtctat cagtacatgc ttgtgctatc
    11701 aatttattta catcttcacg tgtcatacca tatggaaatt ctggaggtct ttgtacaaat
    11761 gatggtgcag cagctgcagc taataccaat tttgacactc catatccatt aaataaactc
    11821 atatacttaa gtacgattgc tcctcccatt gaaaagccaa caagggtaaa gtcttttaat
    11881 ccaatagcat gtaccacctt ataaatatcg tctgctaatt ggctataagt atatcctcct
    11941 gatgttgcat cagatttacc aaaacctctt aaatcaattg aaactgttct atatccgagt
    12001 tttggcaata tatttgtttg atattcaaat atcttatgtc ctaatggcca gccatgtata
    12061 aaaagtacag tttttttagc actcggattc aaatcatata ctgctatatt tatatcattc
    12121 acatatacat aatacatatc aacagcaact cccttcaaga aaatgtatga tggttatttt
    12181 atatatatga ataaaatgat gaataactaa atttttaaat ttatttacaa tacttctcta
    12241 ttgccttttc aaataaactt acatgaagtt tttcatctag gattattcta tttaatatag
    12301 cttttatata agggtcttca ataagactta tatctttata ataattatta atagccacat
    12361 gctccaaatg caaatctagt tttaatctgt tacaaataga atttccataa catacaaaac
    12421 caccattcca atatgcacca caagaacttg tagagcctct atatacagga tttccaccca
    12481 ataatcttat agtctttgct aatatctcca tatgtaacat ttctgttata gatatattta
    12541 tccacatttc acttaactct ctatgcgttt catctaaatc aaaattatgg tatagatatt
    12601 gatttacact cgtaaattca cttgatattc cggcatagtc atccatcaat aattctgcat
    12661 aatacttatt tggacctaaa acttttatct ctggatatgg ctcatcactt gaatatccat
    12721 ttcgcttgtg catgtcgtaa tgtgcatcac tcataatatc tcctccttta aatttaattc
    12781 atgatattaa ttatatgcat cacgactttt tcatataact tagataataa aatctactta
    12841 aataataaaa agccatatta attaaaatta acatgacctt ttatccacaa aattactttt
    12901 ttaattattc atcttttcca gctactgata gctctttaat aacaactgaa ggtgaaccta
    12961 tactactcat tggaaattct aaatcttccc ctatagcttc aatatctttc aacaggttaa
    13021 aatagtttcc tgctactgta atttgctcca ctggaaatac tttttttcct tctttgatat
    13081 aaaaaccttt ggcagctaaa gaaaagtctc ctgttactga atttgcacct gaatgaagtc
    13141 ctgcaaagct agtaaccatc aatccttcat ctatatcctt tataatttca tctaaagatt
    13201 tatctccttt ctctatataa aaattagttg gatatatgct tataggtgat gaatatgaag
    13261 attttattcc atttcctgta gtctttacac ctgccttatt agccgttttt aagttatgaa
    13321 gtaaagttat aagcgttcca tttgaaacaa cttctttttt aaatgtagct actccttcat
    13381 catcaaaagg ggtagatgcc ataccatttt ctaaaagcgg gtcatcaact attgtaacta
    13441 taggagatgc tatcatgtct ccttctctat ttttcaataa agataatcct ttttgagctg
    13501 aatctgcatt aaatatccca gaaaatgtgc taagcaaaga taccatagct tcattaagca
    13561 atatagtttt atatttacct gatggaatac tcttaccacc tactttagat aaagcattat
    13621 caacaccaag ttttgctatc tcacaaggtt ttatttcttc aatagaattt gctatattat
    13681 atcctattcc atcatatttt tgtccattgt cttctataat aggaactaca aaccctataa
    13741 gcatattagt tttattgctt aaattaagac ctttagtatt agatatacca ttacttgata
    13801 cagaataaga tatagtacat ttagataaat ttaccacctt atctgaataa gctttagtct
    13861 ctctttccat ttctaaagct aaatcaatca acttatctgc ctctaaattt tctagctctt
    13921 tagaataagt atttacatca ttatagtgct tatctccttc atatataaat tgtacatctt
    13981 cattttcaat agtgtttact ccatctttta cattttttat caacatatca acagctttat
    14041 catctaaaat ttctgtataa gaataaccta tctttccatt taccttacct ctaaaagata
    14101 aaccaaaaga tttatcaaga ttgtatttct caacttctcc ttcatatata tttatactta
    14161 aattttctcc tgtataataa tatatttcac actcttcaaa accttcactt agagcctttt
    14221 taaataatat atctttaaaa tttttaagtt ccacaattat ccctcctatc ttccacctac
    14281 agtaatttct cttaccctta tcataggttg acctacatta gttggtatac ttcctgatga
    14341 tgagccacac atgccctgag cttgttttaa gttatcccca accatatcta tattcataag
    14401 tacatcactg ccttttccaa ttaaactagc acctctaact ggctcttgta tttctccatt
    14461 ttttattaag taaccttctg aaactgcaaa attaaactcc cctgttacag gatttacaga
    14521 accaccaccc atttttttag cataaagccc atctggtatt gactttataa tatcttctgg
    14581 tttatcttct ccagcatcaa tataagtgtt agtcatcctt gaagttggag caaatttgta
    14641 gttttgtcta cgagaacttc ctgtaggatt cattcccatt cgtctaccat taagtttatc
    14701 tatcatataa gacttcaaaa ttccattttc aatcagtata tttttttgag ctggatttcc
    14761 ctcatcatct atattcatag aaccccagta gtttggtatt gtaccatcat ctatagctgt
    14821 aatctttgta gaagcaatct gttgacctaa tttatctgca aatacagaat taccttttgc
    14881 aactgatgta gcttccaaag aatgtccaca agcttcatga aatattactc caccaaatcc
    14941 attgtctatt gctaccatca tattacctgc aggacaattt ttagcatgca acatagtatg
    15001 cgctactcta gctgattctt ttgcataata ttctgggtct atttcttcaa atatttcaaa
    15061 ccctttacaa cctccaggac cttcaaatcc agtttgattt tcattaccct tagatgcaat
    15121 tgaacttatc cccagtcttg ttcttattct tctatcttca acatatatac cttcagtgtt
    15181 tgcaattaat attttttggt ctttatccaa atagctaaca cttacttgag atatatcatt
    15241 actaaattct tttgcactct tatatgcaac cttcataaca ctcactttat ctttatttcc
    15301 tattgaattt ggataaattt ttatattatg tatgttattt attttgatac tgtcatttaa
    15361 tattacactt tttccatctt ccaatttacc taaagcaaca gcagctttgt atgctacatc
    15421 cagaagacta tttaatgaat tgtcacttgt ataagcatat atactcttta atcctttaaa
    15481 tattcttatt cctataccat aatttcttcc acctattgca ttttctactt tattatcaat
    15541 taaactaata gaattattta tagtgtcttc ttcaaaaatc tcagcaaaat ctcctccagt
    15601 aattagacac ttttctaata ccttagaagc tatatttttt gaaagcataa atttacctcc
    15661 tactttaaat ttttaattaa cttttattca atctttttac ttgttaacaa acattatata
    15721 ctatattac
//
LOCUS       DAEMAE010000081        15543 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEMAE010000081 DAEMAE010000000
VERSION     DAEMAE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.083"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            158..715
                     /gene="efp"
                     /locus_tag="KPH93_001968"
     CDS             158..715
                     /gene="efp"
                     /locus_tag="KPH93_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG1688788.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            807..1262
                     /locus_tag="KPH93_001969"
     CDS             807..1262
                     /locus_tag="KPH93_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1688789.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            1425..2135
                     /locus_tag="KPH93_001970"
     CDS             1425..2135
                     /locus_tag="KPH93_001970"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG1688790.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            2189..3259
                     /locus_tag="KPH93_001971"
     CDS             2189..3259
                     /locus_tag="KPH93_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1688791.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADIIKDYLEFV
                     ILQNKNIKQMLVKANKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            3287..6841
                     /gene="smc"
                     /locus_tag="KPH93_001972"
     CDS             3287..6841
                     /gene="smc"
                     /locus_tag="KPH93_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG1688792.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQKNIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KINNIKNEISHLELKRESLDKNVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSNDVRKNIEELDGLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            6868..8148
                     /gene="ftsY"
                     /locus_tag="KPH93_001973"
     CDS             6868..8148
                     /gene="ftsY"
                     /locus_tag="KPH93_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG1688793.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEDTEEIENFEKIEVGMDINGKKVENISNNDSQEDII
                     EEVENYDEIESKEIEDKEEKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            8251..8619
                     /locus_tag="KPH93_001974"
     CDS             8251..8619
                     /locus_tag="KPH93_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG1688794.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            8616..9968
                     /gene="ffh"
                     /locus_tag="KPH93_001975"
     CDS             8616..9968
                     /gene="ffh"
                     /locus_tag="KPH93_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG1688795.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            10006..10278
                     /gene="rpsP"
                     /locus_tag="KPH93_001976"
     CDS             10006..10278
                     /gene="rpsP"
                     /locus_tag="KPH93_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG1688796.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            10343..10570
                     /locus_tag="KPH93_001977"
     CDS             10343..10570
                     /locus_tag="KPH93_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG1688797.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            10678..11193
                     /gene="rimM"
                     /locus_tag="KPH93_001978"
     CDS             10678..11193
                     /gene="rimM"
                     /locus_tag="KPH93_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG1688798.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNDKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            11202..11897
                     /gene="trmD"
                     /locus_tag="KPH93_001979"
     CDS             11202..11897
                     /gene="trmD"
                     /locus_tag="KPH93_001979"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG1688799.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPVYDTYKHIITTHNINSPSVIYLTPKGKVYNQSIAKQ
                     LSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            12082..12432
                     /gene="rplS"
                     /locus_tag="KPH93_001980"
     CDS             12082..12432
                     /gene="rplS"
                     /locus_tag="KPH93_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG1688800.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            12753..13649
                     /gene="ylqF"
                     /locus_tag="KPH93_001981"
     CDS             12753..13649
                     /gene="ylqF"
                     /locus_tag="KPH93_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG1688801.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            14038..15330
                     /gene="brnQ"
                     /locus_tag="KPH93_001982"
     CDS             14038..15330
                     /gene="brnQ"
                     /locus_tag="KPH93_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG1688802.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKVI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLSSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         6572 a         1490 c         2960 g         4521 t
ORIGIN      
        1 tacttgttta tattgtataa cttatgatat tatgatatta gtaaaaattt gtaatcctga
       61 cttgacagaa ttcaagtaag agatataatt ttaaaggcat agtaatgtaa aatttactaa
      121 aaaataagaa atttatttta atatggagga acaaaaaatg gtatcagcag gtgattttag
      181 aaaaggtgtt acatttgaaa aagatggaca accatgttta gtagttgatt ttcaacacgt
      241 taagccaggt aaaggagctg ctttcgttag aactaaatac aaaaacttaa aaacaggagc
      301 tataagagaa gaaagtttca atccaagtga aaaattccct aaggctgtta tagatacaag
      361 acaaatgcaa tatctataca atgatggtga gttatattat tttatggatc aagaaaattt
      421 tgaacaaata ccattaaact atgagcaagt tgaagatgca attaagttct taaaagaaaa
      481 tgaagttgct acaataagat tttaccaagg acaaccattc caagtggaag ctccaaactt
      541 tgcagagcta gaagttacag atacagagcc aggtataaaa ggtgatacag caagtaatgt
      601 aactaaagca gctacagttg aaactggagc agttgttcaa gttccattat ttataaatac
      661 tggagataaa gtaaagatag atactagaac aggtgaatat ttatcaagag tataaaaatt
      721 tatgtatata aaagcttata tgtcttaata atataagata tatgagcttt tagtttatat
      781 ataaaaaaga aagcgagggg tgttacatga aacatttata tgaagaagtt gcatacttaa
      841 aaggcctagc agaaggacta gaaataagtg ctgaaagtaa agaagggaaa atgatacata
      901 aaatagttga tgcattagaa gtatttgcag atgctattgt aacattggat gaagaacaag
      961 aagaacttca agattttgtt gaatcgatag atgaagattt agcagattta gaagaagata
     1021 ttgctgacat ggaagaagat ctttatgaag aagatgacga tgaggatgat gaggacttca
     1081 gctatataga aatggaatgc ccaaattgtg gagagttagt agaaatagac gaagatttgt
     1141 tatatgatga tgaagtagat gttgtttgtc ctgattgtaa ggctgtgata ttatcatcag
     1201 aagatgattg tgatgatgat tgtacatgtg gtggttgttc aagttgtgat gatagagagt
     1261 aaaaattcat aagaattaaa actgcctatg atgcctatga gatattttgc ttcataggta
     1321 gttttatttt tatgaggaag ggtattatgt attttggttt tgcaatactt ggacaaaaat
     1381 agtacactag aatttataga ataggatttt ggagggatgt aaatatgaaa ataagtaaaa
     1441 aattactaga taatatacaa agatttgaaa atgttataaa ttataaattt aaaaataaag
     1501 aatatatact agaagctctt actcatagtt catattctaa tgaaaataaa aaatataatt
     1561 ttaacgaaag gttggaattt ttaggggatt cggttttagg catagtgata agtgattatt
     1621 tatttaatga agaagctaat ctgcccgaag gggaactaac taaacttaga gcaaatatag
     1681 tttgtgaaga ttcgttaagt gaagtagcta atgatataaa tttaggtatt cacatgttat
     1741 taggtagagg agaagaagca acaggaggaa gacataggac ttcaatatta gcagacgcat
     1801 ttgaagctgt aatagctgct atatatcttg atggaggttt tgaaagtgca agacagttta
     1861 tacttcatca tatggaaaat ataatttatg attcaagaaa aggaaatata tttagagact
     1921 acaaaacgca tcttcaagaa gtgttacaag ggaatggaga aaataatatc tggtacaggc
     1981 ttatagaaga aaaaggacca gaccataata aaagatttgt aatggaagtt ggaattaatg
     2041 acgatgtact tggtatagga gaaggcaaga gtaaaaaaga agctgaacag ttagctgcta
     2101 aaatagcttt aaagaaaaaa ttatgggaaa aataaatata atatgtaaaa atataaaaag
     2161 aaatatttat attagaagga tgttgtagat gaaaaagaga attataccta tatttgttcc
     2221 tcacaaagga tgtcctcatg attgcatatt ttgtaatcaa aaaaaaataa caggtgtgag
     2281 tactgatgtt actagtgaag atgcaagaaa tataattgag gaatgtttag agactataga
     2341 taaagatgca gatgtagaaa tagcattttt tggcggtagc tttactgcta tagatattga
     2401 tatacaaaaa agtttattat cagttgctaa agaatatgta gaaaaaggtc ttgtaaaaga
     2461 tattagaatg tctacaagac cagattgtat tgatgaagat atattaagta tgctaaaaga
     2521 atataagaca agtataattg aactaggagt acaatcctta gatgaaaaag tattattaga
     2581 tagtataaga ggtcatcaat ctgaaattgt atacaagagt tcaaagatga taaaaaatag
     2641 tggaattaaa cttggattac agatgatgat tggtcttcca gctgacacag aagaaaagtg
     2701 tatatttaca gcaaaaaaat ttgtagagct aaaaccagat tgtgttagag tctatccaac
     2761 tttggtagta aaagatacag gtcttgaaaa gttaatggaa caaaataaat ataatccatt
     2821 tactcttgaa gagagtgtaa atatagtaaa aaaagttctt gtattgtttt atgttaataa
     2881 tatcaatgta ataagagttg ggcttcaagc tactgatgat atacaaattg gaaaagctgt
     2941 tttagcaggg ccatatcatc ctgcatttag agagttggta gaagcagata taataaaaga
     3001 ttatttagaa tttgttattt tgcaaaataa aaatataaag caaatgttgg tcaaagctaa
     3061 taaaaagaat atttctaaaa tcataggaaa taaaaaaaca aatgttaaat atatggaaga
     3121 aaaatttggt gtgcttttaa aaacacaaga atcagattta gatataaatc aattagaaat
     3181 tgtgttagat gggaaaagtt taattagtgc taatatgaga gacattcata gaaaattgta
     3241 tgacatttat gacctttagt tttaataatg aagggagaga aaagatttgt atttaaaaag
     3301 actagagtta aagggattta aatcctttcc agtaaaaaca gatattattt ttaaagaagg
     3361 aataacagcc atagtaggac caaatgggag tggaaaaagt aatatatctg atgctgtaag
     3421 atgggtatta ggagaacaga gtattaagag tcttagaggc gataaactcg aagatgtaat
     3481 atttgcaggt acagatacaa aaaaacctat gaattattgt gaagtggccc ttactattga
     3541 taattctgaa aatcaattgg aattagattt tacagaagta actataagaa gaagggctta
     3601 tagaaatggt gaaagtgaat tttttctaaa taataaaagc tgtagattaa aagatataaa
     3661 agaagtcttt ttagatactg gtattggaaa agatggttat tcaatcatag aacaaggaaa
     3721 agtagatgaa atattaagta ataatccttt aagcagaaga aaagtttttg atgaagcttg
     3781 tggcatatca aaatatagat ataaaaaaca ggaagcagaa aggaatttaa gtaatacaaa
     3841 ggaaaattta gaaagaatag atgatgtata tatagaaatt gaaaatcaat taaagcctct
     3901 ttttaatcaa cagacaaagg caaaaaagta cttggaaata agtgaaaaat taaagatact
     3961 tgaagtaaat agctttataa gagaaattga aggaatagaa aaagaattaa gtgaggtcaa
     4021 tgaacatcgt aaggttattg aaaaggagct caatgaaaaa gaagaacaaa aaaatgttgt
     4081 agagaaaaag caagaagata ttaataaaga ggtagaggta ttacaagatg taattgaaaa
     4141 atctgtagat tatataaatt caattaaagg agttatatca aaaaaagagt cccaaataaa
     4201 tttaattaag gaaagaataa gaaattttac gaatgaaatt agtagaaaaa atttagaaat
     4261 aaatgatata aaagagaagc taaatgaaaa taagcaatac ataaaagaat tagaaagtaa
     4321 taaattatca ggtagtgaag aactatctac cttgcagaaa aatataaaag tattggaagc
     4381 aagtaaagat aaacaaaaaa ttaagttaga atccctaaat aatgagatag aattattgaa
     4441 agaaagtata attgatatat taaataagaa acaagagttt agtaataagc tgtccacttt
     4501 aaacgcaaat aaagaaaata tgaatatcag agatgaaaat ataaattcag agataacaga
     4561 gttaaacaaa aatattgaaa taaaatcatc tgaattagat actataaata aagaatttaa
     4621 tatgcaaaat gaaaatttaa agaatgtaaa taatagacat aaagaattat caactaattt
     4681 gcaagattca attagtgaac acaataaact agaagatgaa atacagaaaa gtaaatataa
     4741 tttaaatgga tataattcaa aattaaatgt ctatatagat atggaaaatc attatgaagg
     4801 gtttaataga ggagtaaaag aagtactaaa aaataaaaat ttaaaagggg ttcatggagc
     4861 attaggacag attataaatg tacctgagaa atatgaaaaa tctatagaag cagctcttgg
     4921 tgcttatatg caaaatataa taactgataa tgagtttagt gctaagtctg caataaacta
     4981 tctaaagcag aataatttag gtagagtaac atttcttcca ttaaatataa ttaaatcaaa
     5041 taaaataagt ctaggaaatt taaaagcaaa tactaagttc ataggaatag caagtgattt
     5101 aattacattt gatgaaaagt atagaaatat aattgaaaat atacttggaa gaacaattct
     5161 tataaataat atagatgagg gcataaaatt tgcaaaagaa actggtcata ggtttaaaat
     5221 agtaacttta gatggtgaaa tcttaaatcc aggaggatct ttgactgggg gaagtctaaa
     5281 aactaatggg aatatactat cgagaaaaag atacattaat gaatatacag aaaagataaa
     5341 taatatcaag aatgaaattt cacacctaga attaaaaaga gaatctttag acaaaaatgt
     5401 gaaaaatatc aagaatgaaa ttgattcaca tgaaagtaaa ataaaagatt tagaaaaaag
     5461 tataataata aaaagtacaa gtataaagaa tgttgaatct gaaattgaat cgttaaaagg
     5521 tagtattact aaattagaaa atgagaagaa tgatttaaat tctaacctaa attatacact
     5581 tgaaaaaagc aatgatgtta gaaaaaatat agaagagtta gatggtttat ataataaaaa
     5641 taaagaaaaa attgatgcat tgaatgagga aataaaaaga tacaatgatt tatatgataa
     5701 agaaaaatct gaatttgatg aattgaactt aagtttagtg aagaaaacag aggtttacaa
     5761 tagtatagtt agagatataa aaagaatatc tggtgaaaat tgtgagctag aagaaaagaa
     5821 taaacaatta gaagagtcat taaattatga agaacatgaa ataattaaat tacaagattc
     5881 tatagccact gaagaaaaag aaaaagaaaa tttaacaaaa caactagggg atagtaatag
     5941 aaatcttgaa actagaaaaa tagcaaaaga tgatttaaaa aatagttttg atgaaattaa
     6001 taaagaatta aaaactatag atagacagca tatagagctt aaagaaagtt tatttaaagt
     6061 tggtggaaga ttagaaagat taaaaactag tcaagacaca tatataaata agttgtttga
     6121 gcaatatgat atgactttag tacaagcatt ggaaattaaa gatgaagatt tggatataga
     6181 tagaaaattc ttagaaagtc taaagagaga aataagaagt cttggaaata taaacataga
     6241 ctctataaag gaatatgagg aaataaaaga aagatacgat ttttatagcg aacaaaagca
     6301 ggatttagaa gagtctatgg aagagataga aaaacttata catactttag aagaaaatat
     6361 gaaatctgaa tttgaaatta agtttgaaga aataagtaaa aattttaagt atgtatataa
     6421 gagattattt ggtggaggtt gtggagaatt aactatttta gataaagaaa atttattaga
     6481 aagtgatata ctaataacag ctcaaccacc aggtaaaaaa atgaaaaatt taaacctatt
     6541 atctggtgga gagaaagctt taacggcaat aagcatatta tttgctatat tgattacaaa
     6601 gccaacacca ttttgtatat tagatgagat tgaagcgcca cttgatgatg ccaatatatt
     6661 tagatttgga gaatttttga aggatttatc taaagagact caatttatat cagtaactca
     6721 taggagagga actatggaag ctgctgatta tatctacgga gttacaatgc aagaaaaagc
     6781 catttcaaaa gttattagtt taaaattaaa agaagctcag gaaataacag atattatata
     6841 acttagattg acataggagg aataacagtg ttaaaaaaat tatttggttt tggaaaagac
     6901 aaggaaaagg aaatagaaaa aaaagatgct gaagaagaaa tagaagttga agacagtgtt
     6961 gataatttag aaaacttgga agaaaccatt ttttcaggac ttgaagaaga agtgatagat
     7021 aaagtggaag atattgaaga aaaaactgag gaaagtgtca ataatgaaga cactgaagaa
     7081 atcgaaaact ttgaaaaaat agaagttggg atggatatta atggaaaaaa agtagaaaat
     7141 atttcaaata atgattctca agaagatata atcgaagaag tagaaaatta tgatgaaatt
     7201 gagtctaaag agatagaaga taaagaagaa aagaaagtta acttgtttga aagactaaaa
     7261 caaggattga caaaagcaaa gcaaggaata actgatagaa tagatgaagt tttaaaatca
     7321 tatacaaaaa tagacgaaga attacttgaa gatttagagg aaattttaat aacagcagat
     7381 gttggtgtaa atacaactat ggatataatt gaaagactaa gagataaaat aaagcaaaaa
     7441 ggtattactg aaccaataaa ggtaagagaa gaacttaaat ctatagttga agatattctt
     7501 acaaatgaaa actctacact agatatagaa ccagcaccat gtataatatt aatggtgggt
     7561 gtaaatgggg tcggtaagac tactacaata ggtaaactag caaatagata caaaaaagat
     7621 ggcaaaaaag ttttattagc agcagcagat acatttagag cagcagcaac agagcaatta
     7681 gagatttggg caaatagaac caatgtagac ataataaaac atcaagaagg agcagaccct
     7741 ggagctgtag tatttgatgc tataaaagct gcaaaagcta gaaaaacaga tgtattaata
     7801 tgtgatacag caggaagatt acacaataag gctaatctta tgaatgaact tggaaaagta
     7861 tttaaaatag tagatagaga atttccagaa gcaaagcgtg aagtacttct tgtggtggat
     7921 gctacaacag gtcaaaatgc agttgttcaa gcaaagacat ttaaagaagt tgcagatata
     7981 acaggtatag tacttacaaa acttgatgga actgcaaaag gtggagtagt ccttgcagta
     8041 aaatcagaag tagatgtacc tgtaaaactt attggagttg gagaaagtgt agaagaccta
     8101 caagatttca atgcaaaatc attttcagat gcattatttg gtaactagtg ttcttttaaa
     8161 aataatggtt gacaagagtg ttgttatgat ataaaataca atagtgtaaa gtaaataacc
     8221 tttacagtat actgctagga agtgtaagat atgaatatag aaaaaatggt cgaaattgga
     8281 ttgttatttg aacagtataa agaattatta actgacaagc agaaagaaat agttgctcta
     8341 tactatgaag aagattattc tttaggtgaa attagtgaaa atctgaatgt atcaagacag
     8401 ggtgtttatg ataccttaaa gcgttcagaa aaaatactaa gagattatga agagaagtta
     8461 cacttagtat caaagattca agagcaggaa aaaaatatta agattataaa agataaaatt
     8521 attgacatta aagaagattt attgcacaat agagattgtg ctaatttaat ccctaagcta
     8581 gaaaatatag aagatgtatg tagggagatg ataaaatgat atttgaagga ttgtctgata
     8641 aactacaagg ggcatttagt aagttaaaat ctaaaggaaa acttacagaa gtagatgtaa
     8701 aaaatgctat gagagaagtt aaacttgctc ttcttgaggc ggatgttaac tttaaagtag
     8761 ttaaagattt cataaaaaaa gttcaagaga gatgtgttgg acaagaagtt atgcaaagct
     8821 taactccagc tcagcatgta atcaaaattg taaatgaaga gcttacaagt ttaatgggag
     8881 atgtacaaag taaagtaatg atttcctcaa agcctcctac aattttgatg atggttggat
     8941 tgcaaggtgc aggtaagact actacagcag gaaaacttgg aggatatttt aaaaagcaag
     9001 gtaaaaaacc tctattagta gcttgtgata tttatagacc ggcagctata aagcaacttc
     9061 aagttgttgg agaaaaattg gatatacctg tattcaatat gggagataaa gaaaatccag
     9121 taaatattgc gaaagcaggc ttaagccatg cgattaaaaa tgctaatgat ttagttatta
     9181 tagatacagc tggtagactt catatagatg aagtacttat ggatgagtta aagtctatta
     9241 aatcagaagt taagccacat gagatacttt tggttgtaga ttctatgaca ggtcaagatg
     9301 ctgtaaatgt tgcagaaagc ttcaatgaag cacttggtgt tgatggagta gtattaacta
     9361 aattggatgg agatactaga ggtggtgctg cactttcaat aagagcagtt acacaaaagc
     9421 ctataaaatt tatgggtatg ggagaaaagc tagatgatat agaacctttc cacccagata
     9481 gaatggcatc gagaatatta ggtatgggag atgtcttaag tttaatagaa aaagcacaag
     9541 aaaacataga cttagagaaa gctaaagaat tagagcaaaa aattaaaaaa caagagcttg
     9601 attttgaaga tttcttacaa caaatggaac aaatacaaaa tatgggacct cttgacaaga
     9661 tacttggtat gatacctggt atgggaaatg taaaggacca acttggagat gtagatttga
     9721 atggcaaaga aatgaagaga acaaaagcca taattcagtc aatgactaca tatgagagaa
     9781 gaaacccagg agtattaaat gcttcaagaa agaaaagaat agctaaaggt agtggaacta
     9841 gtgtacaaga tgtaaataga cttataaaac aacttaatga aatgaaaaaa atgatgaaga
     9901 tgtttgctgg ttcacaaaag agtatgaaga aaaggggcgg cttaggcggt ttaccttttt
     9961 ttaaataaaa tataaaaaag taaaaattat tcggaggtga ctgtaatggc agttaaaata
    10021 agattaaaaa gaatgggagc taacaaaaaa cctttctaca gaatagtagt agctgattca
    10081 agagctccaa gagatggaaa atttatagaa gaaataggat actataatcc aatttctgaa
    10141 cctaaacaag taagaataaa tgatgaaaaa gctataaaat ggttagctac tggtgctcag
    10201 ccaacagaag tagttaaaaa attacttgta aaaaacggag taatagaaaa attcgaagct
    10261 tctaaacaag caaaataatt ttttcttggc aattagttta agtaagggct cattgctaag
    10321 gttactttag gagatgaaat atatgaaaga gctagttgta gatatagcta aggctctagt
    10381 tgataatcct gactcagttg ttgttgagga atttgaagat aatgatggta tcgtcttaaa
    10441 gcttacagtt gctcaagagg atatgggtaa ggttattggg aagcagggaa gaatagctaa
    10501 agctataaga actgtcgtaa gatctgttgc aaatagagaa aatataaaag tttctcttga
    10561 gatagtataa aataggagga ttaggtataa tgcctagtcc tttttatata aatgtgtagt
    10621 ttaagatttt atgataatat gaccacataa gattaaattt tttaagaagg tgataacatg
    10681 aaagaaaaat taactcattt taaggtaggt caaatagtaa atacacaagg tctaaaaggt
    10741 gaagtaagag tttatccact tactgatgat atagagaggt ttgatgaatt aaaagatttc
    10801 tatctgggaa aagatttaaa tgataaattg gctgttgaaa aagtaagata caaaggttct
    10861 atggttataa tgaagataaa aggcatagac agcatagaga aagcagaaaa actaaaaaat
    10921 aaatttatgt atgtatctag agaagatagc agagagcttg aagaaggcga attttttata
    10981 gcagatatga ttggaatgga agttttaact gtagatggaa aatatgtagg aatactagaa
    11041 gatgttcttc aatactcagc aaatgatgtc tatgtagtaa aaggtgagga agataaagaa
    11101 tttatgatac cagctataaa aaaatttgta cctacaatag atattgatga aagaaaaatg
    11161 attatagacc ctattaaggg tatgatagat taggtggtaa tatgagattt catataatga
    11221 cactttttcc agaaatattt aattcgtaca tggatgaaag tataatgaag agagcagttg
    11281 aaaaaggtat aattgaggtt catatatata atataaggga tttttctaat aataaacata
    11341 aaaaagtaga tgattatcca tttggaggag gagcggggat ggtaatgact cctcaacctg
    11401 tatatgacac atataagcat ataataacaa ctcacaatat aaatagtcca agtgtaattt
    11461 atcttactcc aaaaggcaag gtatacaatc aatctatagc aaagcagttg tctttaaaag
    11521 aagatataat actactatgt ggtcattatg aaggaataga tgagagaatc attgatttaa
    11581 tagttactga tgaaatttca attggagatt atgtattaac tgggggagag ctcccagcac
    11641 ttataatgat agattctata tcaagattaa taccaggtgt attaaaccaa gaagaatcat
    11701 ttgaagaaga atcatttaaa gataacttat tggaatatcc tcattacact agacctagag
    11761 attttgaagg attaaaagta ccagaagtgc ttttatcagg gaatcataag aagatagatg
    11821 agtggagaag agaagaatct ataaggataa ccaaagaaag acgatttgat ttgtataaaa
    11881 agtctaatga aaaataaaaa tataatgaaa aattaatata tatgtataca ataaagtacc
    11941 tataaatgga attaaaaaca taaaagtgct tgtaataact attgaagtat agtataatat
    12001 aaaatgttga aaatacgggc gttcctctgt ttacactggt cgggtagtta agaacgtcta
    12061 tgatatctag gaggaagaaa gatgaatgaa atgctaagag caattgagca agaacaatta
    12121 aaaaatgaag ttcctaattt tggacctggg gacacagtta aagtacacgt tagaattata
    12181 gaaggaaaaa gagaaagaat acaagtattc gaaggtgtag tattaaagag acaaggtgga
    12241 ggagcaagag aaactttcac agttagaaaa atgtctttca atgttggtgt agaaagaact
    12301 ttcccagttc actctccaaa aatagaaaaa atagaagtta ctagaaaagg taaagtaaga
    12361 agagctaaac taaactactt aagaggtaga gtaggtaaag ctgctaagat aaaagaagct
    12421 agaaataaat aattaaaaac tttacttata aatggggggc tgatttatca gtcctctttc
    12481 tattttaatg aaaattacag caaataatta tactagaaat aaatagaaat atttgttgaa
    12541 aatatacatt atttatctat gaatttatag cttttaaaat gaaaaataaa attggtaaat
    12601 attacattat attttgtaac gatattaaaa ataagagaac agtattacgt aaagtatgac
    12661 gttaaaaaga tgacaatatt atagtttgtg aagatatatt tttttagtgg aattaggata
    12721 taataatata atattaataa ggagtgatac atgtgtcaaa tgaatacgaa gagtatctta
    12781 tggatgacaa tctacatata aactggtatc caggtcatat gaaaaaaact aaggagttag
    12841 taaaaaataa cttaaaattg attgatgtag taattgaact tttggatgct agaatcccgt
    12901 ttagtagtaa aaatccagac atagacaggc ttgtaggaga taagccaaga gttgtcgtac
    12961 ttaataagag tgatatggca gatagggata aattgaatca atggattgaa tactataaga
    13021 aaattaatat aaaagctata ccagtagata caataaaagg tgttggtata aataagataa
    13081 tcgaagaatg taaaaatgta actagagaaa aaatgagttc tttaaaagac aaagggagaa
    13141 aagaaagagc tatacgaata atgatagttg gagttccaaa cgttggtaaa tcatctctta
    13201 taaacaaatt aacaggtaga aaaagcactc aaactggaga taagccagga gtaacgaaag
    13261 ggaagcaatg ggttagatta aagggaaatc ttgaactttt agacacacct ggaatacttt
    13321 ggcctaagtt cgaagaccaa gaagttgcat taaatttagc atttagtaga gctataaagg
    13381 atgagatttt agatactgag actttggctt taagacttat agaaaaactt atgaaaatag
    13441 aacctgagaa gttgaaagcc agatataagt tagattgtct aggagaaact ccaattgaaa
    13501 ctatggatat gattggccat aaaagagggt ttataacagg taaaaaagaa ttagattata
    13561 cacgcatagc cacaactgta ttaaatgaat ttagagatgg aaaaatagga aatataacat
    13621 tggaagttcc agaaaacgtt aaaagataga ttttaaaaca aaatcaaaaa tttaatgtta
    13681 aaaatttaaa aaagcctttt accaaaggct tttttatttt tttgaaatat agacaaatat
    13741 ctccatataa aagtataaat taggttagaa aacctttaaa aatattaatt taagacaaat
    13801 atacctaata ttttgaaaat taaaatgagt atgattattt aaaaatttag catattgact
    13861 tgcttgtttc gtataatata taataagcac aaataaatac aatatattaa aaatttaatt
    13921 tatttaattt tattaattta ataataagtt cttattgttt aattcaactt atttatgcaa
    13981 gcgtaaacaa tcgagacatt ttattaattt aataaggtta aaaaggggga ttttataatg
    14041 ggtaagacca aggatgttat agtatttgga tttgcgttat ttgcaatgtt ctttggagca
    14101 ggtaacttaa tatttccacc ttacttggga ataattacag gtccagaatg gttaattgcc
    14161 ttcttaggat ttacgtttgc tgatgctgga ttggcacttc tagcagtaat ggctacagct
    14221 aaattcgatg gaaacgtagt tgaaatgttt aaaagatgtg gtatgaagtt agggattctt
    14281 ataggttgtg cagatatttt atgtatagga ccattcttag ctataccaag aacaggtgca
    14341 acaacttatg aaatgggtat aatgccttta tttggaactt caattccagt gttattattc
    14401 tgtatacttt tttttgcaat ttcatatgta ttaacaataa gaccttctaa ggtcgtagat
    14461 atagttggac agtttttaac tccagctctt ctaatagctt tagcatttat aattataaaa
    14521 ggtattatat cacctcttgg agatatagta gataaaccta tgatacctaa tgtatttgca
    14581 gaaggtatag gtcaaggata tcagacaatg gatgcttttg cagcaatagc acttgcatca
    14641 gtgctaatag tatctttaaa tgataaagga tattctacta ttagtgacaa gttaaaaatg
    14701 attggtaagg cgggcgttct tgcttgtgga ggattagctt tagtttatgg aggattatgt
    14761 ttccttggag caactgtatc aacaatgtat ggaacagatg cagtacaatc acaagtaata
    14821 gtaaatataa ctgaaggatt gcttggaaat gttgggaaag tgattttagc agtagtagtg
    14881 tcattagcat gtttaactac atctataggt cttacatctg ctacaggtca atatttctct
    14941 agacttacaa aagggaagtt gagttatgaa aaaatagtat tagctgtatc tgtgttcagt
    15001 gcagtagtag catcttttgg tgttggaact ataataaaga tagcatctcc tatattaagt
    15061 atagtttatc caccatcaat tgttttaata attttagctt tcttcaatga aaaaattaag
    15121 aatgataatg tgtacaaagg agcagtatat atgtcattgc ttgtaagtat actaacagtt
    15181 atatctagtt atggagtagc tgttccagta gtaaattctt tacctcttag ttcattagga
    15241 tttaactggg tggtgccagt tataatagct ggaataattg gtaattttat accatcaaag
    15301 agtcaatcta atacacttgg aacaaactaa gttttgttta attaaaatat aatcaattgt
    15361 aaataaatga gttaactaaa agtaaaagga gttatttgta taaataactc cttttatttt
    15421 tagattctat atttaattat ttgtatttaa tatggaaaaa gtataagaaa atatcaaaaa
    15481 aatatatggt ttatttttta acaataatag agattaaaaa agaattttag agtaatttta
    15541 taa
//
LOCUS       DAEMAE010000082        15504 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEMAE010000082 DAEMAE010000000
VERSION     DAEMAE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.084"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(291..1490)
                     /gene="coaBC"
                     /locus_tag="KPH93_001983"
     CDS             complement(291..1490)
                     /gene="coaBC"
                     /locus_tag="KPH93_001983"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG1688803.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GAGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILVGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLAK"
     gene            complement(1495..1761)
                     /locus_tag="KPH93_001984"
     CDS             complement(1495..1761)
                     /locus_tag="KPH93_001984"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG1688804.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(1764..2381)
                     /gene="gmk"
                     /locus_tag="KPH93_001985"
     CDS             complement(1764..2381)
                     /gene="gmk"
                     /locus_tag="KPH93_001985"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG1688805.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(2411..3292)
                     /locus_tag="KPH93_001986"
     CDS             complement(2411..3292)
                     /locus_tag="KPH93_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG1688806.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVARFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(3296..4126)
                     /locus_tag="KPH93_001987"
     CDS             complement(3296..4126)
                     /locus_tag="KPH93_001987"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG1688807.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDREFKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(4413..5447)
                     /gene="ltaE"
                     /locus_tag="KPH93_001988"
     CDS             complement(4413..5447)
                     /gene="ltaE"
                     /locus_tag="KPH93_001988"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG1688808.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            5743..7518
                     /locus_tag="KPH93_001989"
     CDS             5743..7518
                     /locus_tag="KPH93_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG1688809.1"
                     /translation="MALDGLVIHSIVNELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEESEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNTIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIKVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(7722..9374)
                     /gene="abc-f"
                     /locus_tag="KPH93_001990"
     CDS             complement(7722..9374)
                     /gene="abc-f"
                     /locus_tag="KPH93_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG1688810.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTGSYSYISQSENNIEACSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEINIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINDKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYEEYIINRNKKTPSRENQTKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQISKLKNSF"
     gene            complement(9673..10884)
                     /locus_tag="KPH93_001991"
     CDS             complement(9673..10884)
                     /locus_tag="KPH93_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG1688811.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLKLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(11168..11701)
                     /gene="pyrR"
                     /locus_tag="KPH93_001992"
     CDS             complement(11168..11701)
                     /gene="pyrR"
                     /locus_tag="KPH93_001992"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG1688812.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(12061..13008)
                     /locus_tag="KPH93_001993"
     CDS             complement(12061..13008)
                     /locus_tag="KPH93_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG1688813.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKVTVDKPIGRNPKDRLKMAVVKE
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(12977..13423)
                     /gene="lspA"
                     /locus_tag="KPH93_001994"
     CDS             complement(12977..13423)
                     /gene="lspA"
                     /locus_tag="KPH93_001994"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG1688814.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFITVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(13733..14512)
                     /locus_tag="KPH93_001995"
     CDS             complement(13733..14512)
                     /locus_tag="KPH93_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1688815.1"
                     /translation="MKKTIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLVDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKGL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     NKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIEHLDKNKQVQK"
     gene            complement(14567..15049)
                     /locus_tag="KPH93_001996"
     CDS             complement(14567..15049)
                     /locus_tag="KPH93_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG1688816.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            15334..>15504
                     /locus_tag="KPH93_001997"
     CDS             15334..>15504
                     /locus_tag="KPH93_001997"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1688817.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAF"
BASE COUNT         5099 a         2707 c         1589 g         6109 t
ORIGIN      
        1 ttgcatattt tttcattata aaacctctac ttaaatttaa tatattgttc atattttaat
       61 atactttaca agatattaaa atctcagaat ttataagcaa tttcaagtta ttaatattaa
      121 ctttatttta ataattaact attaattata ttttatactt tatgacaaaa taaatgctga
      181 aaactacaat ttaactgaca tttgcgagtt tttactcgct aaaattaaat tgcagctttc
      241 agccacttta gctgtgataa aatacacaaa tatcatattt aaaaaaggtc ttatttcgca
      301 agcaacgctt taactttatc taaaataaca ttggctactt cttctttttt catcttagga
      361 tactcagtaa tatttccctc tacatctatt atttttacaa tattagtatc agctctaaaa
      421 ccagcacctt ctttagttaa atcatttgcc acaataaaat ctaaattttt cttttttatt
      481 tttcctttgg catgttctat aaggtcatta gtttcagctg caaatccaac tagtatctta
      541 tcttctttta tttttcctat ttcataagca atatcataat ttctacttag ttctattgat
      601 aaatcatcat ttttcttttt aatttttttg tcagaataag tttttggttt ataatctgca
      661 actgctgcac tttgaattac tattttattt tcacttaaat ttgatactac tgcttcgtac
      721 aaatcttgtg ctgactctat ttgaacaaat tttttaagat tttgtgggat agaaagattt
      781 gtaggacctg atattaatgt tacatcagca cctctagcta tagcttctct tgctatagaa
      841 tatcccattt taccagaaga cctattagtt aaatatctta ctgggtctat actctctaca
      901 gttggaccag ctgtaactat catactttga ccttttaaat cttgctcttt agtaagatta
      961 gttaaaactt cctctactat tatatctgga ttagctagtt tacctgctcc aatatctcca
     1021 catgcaagtc ttccactttc tggttcaata aatttatatc caaaactctt taaagtttgt
     1081 atattttttt gaacaattgg attttcatac atattagtat tcatagctgg tgctataaga
     1141 acctgtgctt ttgttgccat aacagtagtt gtcaacatat cgtcagctat accatttgca
     1201 atcttgccta taacatttgc acttgctggc actattaaga ataaatctgc tttttttgct
     1261 aatgatatat gttcaacttc ccaagttttt ggttcttcaa acatatcttc tactacataa
     1321 ttttgactca gtgattgaaa tgtaagtggt ttaacaaatt ctgttgctga ttttgtcatt
     1381 ataacatgga tatttgcatt tagttttttt aacttactta caacatcaca agccttatat
     1441 gcagctatac cgccacttat gccaattaca acagttttat cttttagcat gaccttactc
     1501 ctcttctgaa atttgttggt gttgctcagc atgatgtcta gcttcttcta tctctatttc
     1561 ctcttctgtc aataatctat atgttatttt tccacttgca acttctttag ttgctacaca
     1621 tacaggcttc tccttagttt tatttgaaac atatggttct tctccatcaa ttaattttct
     1681 tgctctcttc gaaacagtcc ctactaaata gtatctgttg tctattttct ctagtacctc
     1741 attgatagat ggttttaaca taattataat tcctccttaa atttctctat tataatattt
     1801 ttatatctgc taactttatt cttttcagat gatattatac cttctatttc tttagcagat
     1861 gtttttacat cttcattaaa tataaaataa tcataatcct taatcatttt aatttcttca
     1921 tatgcacagc taaatctctt ctctatttca gcttctgttt ctgtacctct tccaacaatc
     1981 ctatttttta attcttcaag tgatggtggt agtacaaata taaatactcc ctctggacaa
     2041 acttctttta tttgttttgc tccttgcatt tcaatctcca gtaatacatc ttgtcctttt
     2101 tctaaacatt ccattattgc tgcttttgga gttccataaa aattatcata aatttgagca
     2161 tactccaaaa actcaccttt ttctatcatc tcttcaaact tttccttact tatgaagaaa
     2221 taatttactc catcaacttc tccagttctg ggttttcttg tagtagctga tacagaaagt
     2281 tttattgtat cattttcttt aagtagttct ttgcaaatag taccttttcc tgcacctgat
     2341 ggtcctgata caactagcaa taatcctttt ttcacaaaca tttaagcatt ccccttctaa
     2401 tattgtattc ttactctata ttttgtattt gttcccttat tttttctaat tcgcttttta
     2461 cctctactac taaattagtt atatttatat cagatgactt cgaacctata gtgttagttt
     2521 ctctattcat ctcttgtatt aagaaatcta tttttctacc tatagagtcg tctttaaata
     2581 tagtattttt aagttgccct atatgacttt taaatctaac tatttcttct gttatactgc
     2641 ttttatctgc atatattgca acttcttgtg ctaatctatt ctcatctatt atacttgggt
     2701 catccagtaa ttcacttatt ctcaaattca atttttctct ataatcttct acaacactac
     2761 ttgaatattt ttcaatctct tctatatgat tttttaataa atcacatctc atggcaatat
     2821 cttcagctaa ttttttacct tcttcacttc tcatttctct taatttgatt aatgcatctt
     2881 caactgcttg attcaacatt gaccataata aatcttcatc ttcttccttt tcttctattt
     2941 ttacaatatc aggaaacctt gcaacattca taacgctaat atcatctata atgtcaaatt
     3001 tattttttat ttcttttagt atgtctatat actgagatgc taatccctca tcaaaattta
     3061 agtttacatc ttctttgcct aataagtcaa actttatgta taaatctact cttcctcttt
     3121 ttacataatt ttttactaaa tttctaactt tatcctctaa aaatgaaatt tttcttggaa
     3181 gacgtatgtt tatatctgaa tacttatgat ttatcgtttt acactctacc aaaaagtaat
     3241 aattatcatc cttgtattct cctcttccaa aaccagtcat acttatagcc attaattaaa
     3301 cctccaaaac accttcacat atttttactg ctggacctgt catataagag aaatctccta
     3361 catctatttt aactgttcca ccttgagacg ttacatttac actactttct accatattaa
     3421 gtaaattgca cactattgct gatgcagtca taccagtacc acaacctaga gtatatccac
     3481 aacctctttc ccaagtataa acatgtatat tatacttatc ttctaccttt acaaaattta
     3541 cattcgtttt ttcaggaaat attttattat tttcaatctc ttttccatat ttacaaacat
     3601 catctacatc taattcatct actataacta ctgtatgagg aacccccatc aacatagaag
     3661 taactttaaa ctccctatct aaaaccttta tactttcttg cacaaaacgc tctttatttg
     3721 taagtacagg aacgtcctta gctataaaac ttccttttcc catattgact cttatagagt
     3781 ttatttctct attggtaaga tttattttta tatcttttat accatctaat gtatctactg
     3841 taaaactttc ttttttaact atacaattat cataaacaaa tttacaaaaa catctaaggc
     3901 catttccaca catagctgct cttgaaccat cagaattata atataccatt tctacatctg
     3961 caacttttga atctttcaca actaacaatc catccgctcc tacagaaaat cttctatggc
     4021 atactttttt tgctaaagca gtataatcag atggatttat ctggtcgaat cttccatcta
     4081 ttgctataaa atcatttcct attccatgta gtttccaaaa tttcataagt atcgctcctt
     4141 cgtaaattaa cacttaaact attataacat aaaaaaattc ccataaactt ataatttatg
     4201 ggaaaagttt tgtaatgaca actataattc gaggatgtga gggagagttg ccattacaat
     4261 tagggtaatt atattatgtt tttaagctag ttctttctat gtttatatat taatatttct
     4321 atatgaattt tatgtatgtt ttatttaaac tgtttatgaa ttttttaatt aaacatctgg
     4381 taaccagcta taatcctttt agtagatatc tattaacata cacctattaa ttctttaagt
     4441 gtttttaaaa catagtctaa atcttccttt gtaatatcta catgagttac aaatctatat
     4501 tcaccatttt ccattttatt aatttttata tttctttcaa aaaattcttt tattaaaaca
     4561 tcctctttta taatatcttc actcatctta aaaaatacca tacttatatc tggattaaca
     4621 ttatttactt caatgccatc aattttattt aattcacaag ctaaatattt tgcattttca
     4681 tggtcttcat tcaatcttaa agtcattttt tcaatggcaa ttatacctgc tgctgcaact
     4741 atacctactt gtctcattcc tccacccata actttttgct ttcttctagc ttttgctata
     4801 aattctttac ttccagcaag tatagaaccc attggtgcag ctaaaccttt tgacacacag
     4861 aacataacac tatcacaaca ctttgctatt tctttagctt ctacacccaa agttaatgct
     4921 gcgttaaata atcttgctcc atctaaatgt actggtaagt tatgtttctc agctattttt
     4981 ttaatttctc ttaaattatc aaggctcaca acagttccac ctgaatatgc attttctaca
     5041 catataagcc ctgtctgtgg ttcatgtatg tcatctcttc taattgcctt ttcaacaagt
     5101 tcaggattta gagctccact ttcgtcttca tctatagttc taagctgaac tccagctatt
     5161 acagcaggtg ccccaacttc atgagttaca atgtgataac ctttaccaat aattatttct
     5221 tgccctctta ggcaatgagt gaatagagct aattgattcc caaaagttcc acttggaagg
     5281 aaaatagcat cttcttttcc aactttagct gctgctaatt cttcaagttt atttacagta
     5341 gggtcatctc taaagattga atctccaaca ggagcctttg ccatagcttc tctcatttcg
     5401 tcagtaggca tagtaacagt atcacttctt aaatcgataa atctcatttt ttcaaccttc
     5461 tttcatattt atatactata gaaccttaaa attgattttt tctgtaaagt tcatatactt
     5521 ttatagctta tactctacac aataattata ctatatttac tttatattta acctacttta
     5581 aattattaat tttaaaaata aagtttcctt tctaataagt ataaataatt atacaattta
     5641 aatattaatc tctattgttt aaattccccc tagtcacaaa gcgtatatta tgtgtataat
     5701 taatttactg actcattttg gtcaaagaaa ggggtgctat aaatggcatt agatggatta
     5761 gttatacatt caatagtcaa tgaattatca tcaaaactta caggtggaaa aatagataag
     5821 atacatcaac ctgaagatga tgaggtaatt tttaatataa gaaataataa agaaaatttt
     5881 agattggttt taagtgcaag tgcctcaaat cctagagttt atcttacaag taattatcaa
     5941 aaagaaaacc ctctaaaagc gcctatgttt tgtatgttac ttagaaaata catacaaggt
     6001 ggaaatatag tagaaatttc tcaaataggt tttgaaagaa taataaaaat aagtgttgaa
     6061 tctttagatg aattaaaaga aaagacagtt aaaaatatta tgattgaaat tatgggaaga
     6121 catagcaaca taataataac tcatggtgaa gaaaataaaa ttatagattc tataaaaaga
     6181 gtccctttta gcataagtag agttcgtcaa gttttacctg gtcatgatta ttcattacca
     6241 ccagaacaaa acaaactcaa ccctcttgat gatatatcta aggatttgtt tattaaaaac
     6301 ctagaagaat cggaaggacc tatctttaag tctatatatt ctaaatttct aggtataagc
     6361 cctattatag ctaaagaaat ttgctaccga gcaggtgtaa atcaaaatac tatcataaag
     6421 gatatatctg atgaacaatt tgattcttta cacaaagtat tttgtaattt attcaatgat
     6481 ataaattcta acaaatactc accttgcatt attatagata aaaaggtgga taaggtagtt
     6541 gatttcagtt gtataaacct tacattattt agtgatttat catatataaa taaggatagt
     6601 atgtctcgta ttctagaaga tttctataga actaaagata ttaaagatag aattaatcaa
     6661 cgttcatctg atttaaagaa gagtatatct gttaagcttg atagacttta taataaactt
     6721 aaaaaacaag aagaagaatt atcagaatct gaaaatgcag acatttataa gataaaagga
     6781 gaattgatta cttcatatat ttatatggta gaaaaaggta tggaaagtat taaagttgct
     6841 aacttctatg atgaaaattg taatgatgta acaatagaat taaataaaaa cttgacacca
     6901 tctgaaaatg ctcaaaaata ctttaaaaaa tataacaaaa tgaaacatgc taaagtcgag
     6961 atatctcatc aaatttcctt aaataaggaa gaaatagatt acttagaaaa cataatttta
     7021 agtattgaaa actgtgaaaa tttagctgaa ttacaagata taaaagaaga acttgctaag
     7081 gttggatata tcaaaactca aaagaaaaat agtaaaaaag atactatccc atctacaaaa
     7141 cctcatgaat ttgtgtcttc tgatggattt aaaatattgg ttggtaaaaa caataagcaa
     7201 aatgactatc ttacactaag acttgctgat aatgatgact tatggatgca tacaaagaat
     7261 attcctggtt ctcatgtaat aataaagtgt gctggtaagg aagtccctga taatactgta
     7321 tttgaaggtg ctatgctagc agcattcttt agtaagtcaa aactttcttc tcaggtgcct
     7381 gtagactata ctaagagaaa aaatgtgaaa aaacctagtg gttctaaacc aggtatggtt
     7441 atttatgaaa ctaacagtac tatttatgtt actcctgaag aagaaacagt agccaagctt
     7501 aaggttaaat ctgaataaat tctgcactaa atacaaaata agtgtcagac aagtacacat
     7561 tatcattcta aattaaaaag tggccttatg tacataagac cactttttaa ttagctcact
     7621 tttaattttc taataaaata tttttctgtt ttaataacta tacttatatt cccaacaaat
     7681 attttcagtc ttcttatata gatataatta aaaagttaat actaaaagct attttttaac
     7741 ttactgattt gttttagtag ttcattgtat tctgattcgt acaattcttt ttttaagtta
     7801 tctttttcta tagataacat agatataaca gttgtaagtc tattttctaa tactagaaga
     7861 ttttcttttt tggtttggtt ttctctacta ggagtttttt tatttctgtt aattatatat
     7921 tcttcataat tacaatcaaa ctcccttatt ttagcatctt ttatctctat gatatagtta
     7981 caaatatgag atacaaatac tctatcatga gacactataa gcatagtttt atcagtattt
     8041 atcagtgcct tttctaatgc ttccatagat tttatatcta agtaatttgt tggttcatca
     8101 aatactaaaa aattattatc ttccaatatt attttgcata gtgctatttt taccttttct
     8161 ccaccactaa gtacttttac ttttttgtag acatcatcac ctttaaatcc aaacaggctt
     8221 aagtttatcc ttataaagga ttcatcaaat gaactattaa ctttagtatt gtatagaaca
     8281 ctcttttcct catctaataa actttgattt tggtcaaaat aacctacttt taccttgtca
     8341 tttattttta tattgtcatt tttatcagcc aatatttctt ttatcagagt acttttccca
     8401 cacccattat cacctaataa tgctatcttt ttacctcttt ttatcttaaa ggaaacatta
     8461 tctaacagga gcttattttc taactttaga gtcatatctt ttacctctac tagatttttg
     8521 ctaattatct ccataccatc ttgaatctta atgtttattt ctttagaaac tttaggttta
     8581 ttctttacct caagtttatc aattctgctt tttatagcct tcacatttga gtccaacttt
     8641 ttcttattag tctgaccacc cattttgtgc aacctagctt ctgaattacc cattcttttt
     8701 ggtgtttttc ttatgccatc acttaaagct tttttatata aaagagcttt ttcaagcctt
     8761 tttttctctt taacatattc ttgatactca aaatcagctc tttttacttc ttcatctttt
     8821 tgcattaaat aatcagtgta attgcctttg taagctttta gttttccatc ttctagttct
     8881 aatatagttg tacataactc atccagaaaa cgtctatcat gagatattaa taataatgct
     8941 ccctcatgcc ttttaaacat atcttccaaa actccaatgc tttttttatc taagtttgaa
     9001 gttggctcat cagctattat gatatttttc ttattactca gtgcatctgc tatttttaac
     9061 ttaacctttt cccctcctga taaatattct tcaaatttat ctggagcatt taaaagactc
     9121 ttctccttac tatgactaca tgcttcaata ttattttcac tctgacttat ataagaatag
     9181 cttcctgtaa ggtaagtata tccttcatca atttctattt cacccaatat tgcttttaat
     9241 aatgttgttt ttccttgacc atttgaacca actagaccta ttttgtcatt ttctaatatt
     9301 tcaagtttat ctatatccaa aattaattta tctgcataat atttctttaa attctctact
     9361 tttactaaca acataaaaaa tccctcctta acacacttaa atataaggag agattctata
     9421 aataggcata acaaataaac gtatagacta taatctactc tccttaattt aagcatcgca
     9481 aataaagcca ccaaaatagg cttttcaaaa attacaatac tttaaattat aaaaaataga
     9541 ttatattctc attgtcccag ttaccttgcc ctttctttca tttattttta atcattatat
     9601 caacattaat tacaaatgtc tatagaaaat taactaattt tagttattca atatacaatt
     9661 tattctttaa atttattctt cttcaacttt aaaatcctga ttatttaaaa ttctgtttaa
     9721 tacaattcca acaattgcag ctaaactaag accagttatc tttactgtac ttgttatagg
     9781 gattccaata gaaatgcctt tattagataa atatgttgtt ccaagaccta ttaataatat
     9841 aactgctatt attaatatat ttatcttact ttctacacaa gaactgtccc ttatagtttt
     9901 taatcctaca taagttatca ttgaaaatag cattatacta attccaccca taactgaacc
     9961 tggtatactt tgaagaaatc caccaaactt tcctacacat gcaagcaata tagcaaatat
    10021 tgctgtacgt ctaagtattg aagggtcata gtttttagtt atagcaagta ctgctgtatt
    10081 ttctccataa gttgtattgg ctggtcctcc taaacaccct gcaactatag tcgcaaatcc
    10141 atccccaagt aatgttctat ttaatccggg attttctata aagtttttac ctactacagc
    10201 accattagta gtcatatctc ctatatgttc cataaataca gctaatacaa ctggagatat
    10261 aatcacaatt gctcctaaac tgaacttagg aactgtaaag ttcggtattg caaataaact
    10321 tgcttctgta atcgtagtta tatctactaa ctttaacagt aaacagatta tatacccaga
    10381 aaaaacggct attaatattc ctaattgttt tataaatcct ctaccaaact tattggttaa
    10441 aattgctatt gctaaagtta taaatgctat tacaaaattg gctgaagcca tactaaaagc
    10501 tgttggcaat aaatttaatc ctataactac tatcatagct cctgtaactt gtgcaggaaa
    10561 atataattta attttattta caccaactat ttttactata atcgacatta taatataaat
    10621 taatcctgct actattatac caccttgagc atatgctaaa tctccaccat acgcctcttt
    10681 ggctgctagt ataactggta taaacgcaaa acttgaacct aaaaaggcag gtacttttcc
    10741 ttctgtacaa aaatgaaata tcaatgtacc aacaccagca cagaaaattg caactgacgg
    10801 attaaaacct gtcaaaatgg gaactaatac tgtcgctcca aacatggcta atagatgttg
    10861 taaagacatt atcgtttttt tcataaaaac ctccaaaatt attatatata aataaatcct
    10921 ctgtttaatt aatatctttt aattaattat cattccctag attctatttt ttaacctcta
    10981 tatacttatt atatgatttg tcattatttt acattttacg acatcaaaaa ctcaaataaa
    11041 ggagaataaa gactacatta agactcacat ccataaaatt ataatattta ttttgtgcaa
    11101 tctatgtaag ctttatcctc ctccctgact attcaatgtt aagcttttat ataatactta
    11161 aaaataatta ttctttgatt gtaactgaat cttctccatc aatttctaat aaactgacag
    11221 atataatttc atgtctagaa gtaggtacat tttttcctac ataatctgct cttataggca
    11281 actctctatg ccctctgtcc actagcactg ctaattgtat tgatttaggt cttcctatat
    11341 ctataatagc atctagtgaa gctctcacag ttcttccagt gtataaaaca tcatcaacta
    11401 aaactactgt cttatcattt acattaaaat ctaaataagt tcctttaact acaggttcaa
    11461 catgaatttt ttccaaatca tctctataca aagttatatc aatatctcct gtatctactt
    11521 ttgttccttc tatttgttct attttcttag aaattctatt tgcaattggc acacctctag
    11581 tctttattcc aactaaaact agattttcta cacctttatt tctttcaatt atttcatgac
    11641 ttattctagt aattgctctt gctattgcct tttcatccat taattgagct ttttctacca
    11701 tatcataatt attgtcaaag atataatata tctatttgac aaatctactc ctttctttta
    11761 attttttata aatttatttt tatttaaaat ttcaatttaa acaacaaaaa aacttctcat
    11821 cacaagacaa gaagctctta tataaaaaat atacagtgta atttagcaat tgtcttacga
    11881 tatctctgta tcgtttaaag ataatttttt aatctatcaa ctcaaattat atatttatat
    11941 ttcaaatttt tcaagctttt ttaaacaaaa tactttaata taagatattt tatttaaatt
    12001 tcactaaatt tatttactta tataaaaaca aaaaattatt tataggtatt gtacaaatgg
    12061 ttattttatc ttcttaataa cttccttaaa atactcaggt aattctgaat taaattcgat
    12121 atattcctta gtagtaggat gtataaatcc caacttctta gcgtgcaaag tttgtccttt
    12181 taatttaaat ttagtttctt ttggaccata tacataatca cctaaaagtg ggtgatttat
    12241 agacaaagca tgaactctta tttgatgagt tctaccagtt tcaagcctag ctctcatatg
    12301 tgtaaactta tcaaatcttt ctacaacttc aaagtgagtg attgcatttt ttccctcttt
    12361 tactacagcc atttttaatc tatccttagg atttctccct ataggtttat caacagttac
    12421 tttatcttct ttaaccacac catagcatat aaattcatac tccctagtta tagtatgttc
    12481 ctttaactgt tctgctagaa aattatgcgc ataattgttc ttagctatca taagaagacc
    12541 agaagtatct ttgtctattc tatgaactat accaggtcta ataactccat ttattgatga
    12601 caacttatct ttacaatgat ataaaatccc atttaccagc gtattttcat agtttccagg
    12661 agcagggtga acaaccatat cctggggttt atttattatt aagacatcat catcttcata
    12721 tacaatatct attggaatat cttgaggtaa aacttctaat actttaggtt ctggtatctc
    12781 tataacaata ttatcttcct ctttaaccag atatttagct ttttctactt tattattaac
    12841 ctttactttt ccatctttta taattttttg tatataactt cttgacatat cacctaattg
    12901 ttctgctaga taaatatcaa gtctaacttc ttcttcctct tcaagtacta aaaactcttt
    12961 tacttcatcc attatatcac ctacttttac tttcaaaaaa taaaacatat atacataaaa
    13021 acacagttcc tacaactaca aatacatcag ctatattgaa tacatattcc catataattc
    13081 taaagtcgaa gtaatctact acaaaaccta atcgtactct atcaactaga tttcccaatg
    13141 caccagatat aattaatata attcccaacc ttccaaatat atgtactttt cttgtattaa
    13201 gatagtataa tccaaatact gttgcaagta atgcaacagt tataaatatc cattgattat
    13261 tttgcaataa tccaaatgct gcacctctat tttcgacata agttaaatga aatacattat
    13321 ttattattgg tattgttgaa acatctacca aattattcaa tacccatatt ttagacagtt
    13381 ggtctaagcc tatgagtaga attattatta atatatatag caatgatttt tccccctata
    13441 aattatttaa aaaccaattt tatttgaaac acactattat tattatacaa aaaaaatgct
    13501 ctaaagagca actttattaa aattgtattt tcttaataaa gctagaacaa aatttaaaat
    13561 agtatcactt tttttatatt gtagatttct acatatttaa aatcatatta ttatgtataa
    13621 aaaatttaaa aataccatac taatcaattc tttttgtgta ttttatagat taaagattaa
    13681 tgatgtctgt actttaaact tacaagcacc actaatcttc aaacaaatat ttttactttt
    13741 gcacttgctt atttttatct aaatgttcta taagttcaga tactgttacg aatttatatc
    13801 ctctctcttt taatttagga attactactt ctaaagcttg tatagtttgt gatttatcat
    13861 ttcttttagt gttataatca tgtaaaagta ttatattacc attttggacc tttgtaagga
    13921 tagtatttat aattgtatct actccaggat tagaccaatc tctagggtct aagtttgacc
    13981 aaagaattat attcatgcct ttagaaacta taatatcaca gacactttta ttcatggctc
    14041 tatatggagg tctaaatatc tttggttcta ctcctattac actttttata atattttgtg
    14101 tatctgttat ttctttttca acactaccat aactattttt tgcaacattt atatgtgtaa
    14161 atgtatgatt ccctatttca tgcccctcat catattgtct ttttaataat cctttgttat
    14221 attctacttt ttctccaact acaaaaaaag ttgcttttac atcatttttc tttaatatat
    14281 caagtacttg tggtgtaaaa tcctcatctg gaccatcatc aaaagtcaat gctaaaattt
    14341 tttcatcctc atttcctttt tttattatta catcttcata atcatctttt atattatcaa
    14401 ctaaactata tgcttctttt gaactatctt gattatgtgt attatgatta tatgtatata
    14461 cacctactat agataataaa catatacata taccccatat tgttttcttc atgtcattac
    14521 ctcttttcta attcattgta gcaatatatt atataatgtc agttttttaa tctctcaata
    14581 tcccattctt atttaaatca gttaaatttg ttaggtcttc tgaataaaag ttagaatctt
    14641 ctacatcagg gttttgcatt gcatcaccta ataaattatt ttctttcttt gcacaatcat
    14701 tacataaact tgtatctggc aatatatcca atctttccaa atctatttga ttatgacata
    14761 aatcacaaat cccatatgtt ccattctcta ttttagataa tgctaaatct atttgataca
    14821 atcttccctc ttcatgattt attaaactgt tttgcatatt ttgcatatat aaatctgttc
    14881 ccatgtctcc tatatgatta tcataactag ataattctcc tgatgaatac ttttcacttg
    14941 tatgtttagt tgtatctcca aataaagtat tatctttcat atcctctact aaattagtta
    15001 aattttctct ttctttcaat aatttatctt tatatttttt agtatccatt tttaacacct
    15061 ctaattttaa tatttatatc ataaaaatag tatgtggaaa agtaaattaa acatacaata
    15121 aaaataagat ttgattcaaa aaatgaaatg tctcaaaatg aaatttttag ttcatttaac
    15181 ctcctttttt gtatgaaatc aaacatattt ttatcctttc aacaatgaaa aagagactta
    15241 tctctatttt agtataatcc ccttatagta gacatttaaa taaaaggcta ttataatagg
    15301 tctaaaaagc gtatactcta agggggtttt tctatgtcta aaaaacacaa aaaatatagc
    15361 aaagaactta aactaaaagc tgtaaacttg tatataaaag aaggatactc atcatacaaa
    15421 attgctgaga tgctgaatat aagaagcaaa acacaagttc aaaactgggt aaaagattat
    15481 aaaaataaag gtaaaactgc attt
//
LOCUS       DAEMAE010000083        15459 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEMAE010000083 DAEMAE010000000
VERSION     DAEMAE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.085"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(164..1261)
                     /gene="ychF"
                     /locus_tag="KPH93_001998"
     CDS             complement(164..1261)
                     /gene="ychF"
                     /locus_tag="KPH93_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG1688818.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1577..2842
                     /locus_tag="KPH93_001999"
     CDS             1577..2842
                     /locus_tag="KPH93_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG1688819.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADVDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFAFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2857..4143
                     /locus_tag="KPH93_002000"
     CDS             2857..4143
                     /locus_tag="KPH93_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG1688820.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLGVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEDYCVISIVEPK"
     gene            4541..5281
                     /locus_tag="KPH93_002001"
     CDS             4541..5281
                     /locus_tag="KPH93_002001"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG1688821.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNNISEE"
     gene            5311..6246
                     /gene="rsmH"
                     /locus_tag="KPH93_002002"
     CDS             5311..6246
                     /gene="rsmH"
                     /locus_tag="KPH93_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG1688822.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            6730..7077
                     /locus_tag="KPH93_002003"
     CDS             6730..7077
                     /locus_tag="KPH93_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688823.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            7089..9068
                     /locus_tag="KPH93_002004"
     CDS             7089..9068
                     /locus_tag="KPH93_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG1688824.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            9235..10608
                     /locus_tag="KPH93_002005"
     CDS             9235..10608
                     /locus_tag="KPH93_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG1688825.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            10649..11617
                     /locus_tag="KPH93_002006"
     CDS             10649..11617
                     /locus_tag="KPH93_002006"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG1688826.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            11636..12991
                     /locus_tag="KPH93_002007"
     CDS             11636..12991
                     /locus_tag="KPH93_002007"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG1688827.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELESINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCI
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIRDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            13043..14173
                     /gene="spoVE"
                     /locus_tag="KPH93_002008"
     CDS             13043..14173
                     /gene="spoVE"
                     /locus_tag="KPH93_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG1688828.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            14191..15417
                     /gene="murG"
                     /locus_tag="KPH93_002009"
     CDS             14191..15417
                     /gene="murG"
                     /locus_tag="KPH93_002009"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG1688829.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEEKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMESISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
BASE COUNT         6091 a         1687 c         2831 g         4850 t
ORIGIN      
        1 aaaaaagaga ggttttcata atgattatta taaatcattt agaatagctc tcttttttta
       61 aaaaatttat ataattatat aaagataata aaaaatctat taattaagtt taattttcta
      121 attatcatac atacaatagt taaggtgtat atgtttaatc attttaaaca ttaaatctaa
      181 ataatataac atctccatct ttaactatgt attctttacc ttctaattta accaaccctt
      241 tttctttagc agcagccata gtaccatgct caactaaatc attaaaggct attgtttctg
      301 ctctgataaa tcctctttct atatcagaat gtatttttcc tcctgcctga ggagctttac
      361 ttccaacttt tatagtccat gctctaactt cttttggacc agcagttaag aatgaaatta
      421 aaccaagtaa agcataagaa gattttataa gcttatcaag ccctgattgt tcaagaccta
      481 atgtttctaa aaactctttc ttttcttcag cagaatcaag ttcagaaatt tctgcttcta
      541 tttgtgcaca tactacaaca acttcggcat cttctgtttc tgcaaatgct ttaacttgtt
      601 gaacatattt attattttct ccattatcag ctaaatcatc ttctgataca tttgaagcat
      661 atattacagg cttagatgta agtaaatcca acgaatttac gaatgtttgt tcatcttctg
      721 taaattccat agttcttaca cacttacttt cttctaaagt agacattatt gattttaata
      781 aatcaagttc agaacctaaa gttttatcag ccttagcagc tttttgagtt ttattaattc
      841 ttctttcaag aatctctata tctgagaaaa ttaattctaa gtttatagtt tctatatcgc
      901 gaagtgggtc tacagaacca tctacatgta caacattttc atcctcaaaa catctaacaa
      961 catgaactat tgcatcaact tctcttatat gtgataagaa tttatttcct aagccttctc
     1021 ctttagaagc acctctaact aaacctgcta tatcacaaaa ttctatagca gtaggaacta
     1081 ttttttcaga attatataat tcttgtagtt tgtttaatct ttcatctgga acagatacaa
     1141 ctcccacatt tgggtctatt gtacaaaaag ggtaatttgc agattctgca cctgcttgag
     1201 ttattgcgtt aaatagtgtg cttttaccta catttggtaa accaactata cctaatttca
     1261 tattaatcgt cctctctctt attctttttt atattcttta tataacttta aacaatcaat
     1321 ctataggatt gcaaaattgt tttaattata gacatacata ttgattatac atcatatata
     1381 tcaagcttgt aaactagact ttaaagcata ttgatttaaa taatatttgt atttaattta
     1441 aataaaaata ttgcttttag aaaataataa aaaggatata attaaataga aggtttttat
     1501 atgtgagtat atgtaattaa aaaataaata aatttcaatt ttaaaattga tgtttaaata
     1561 aaaataggag gtacatatgg ttaatactaa aaaaataata aatttaggca ataatattaa
     1621 tcttactttg ataaaaacag aaaagtttaa atcgaatctt ataagtttat atgttcagag
     1681 actattggat gaaaaagaaa ctacaaaaaa tgctctaata ccatcaataa tagcaagtgg
     1741 aagcgctaaa tatcctagtg ctagagcaat atccaataaa ttagatgatt tatatggttc
     1801 atctatggga gctgatgctg taaaacgtgg agaacgtcaa gtattgagct ttaaagtaat
     1861 taacataagt gaaaagtatt tagatgagag tatatttgaa gaagtagtag aattttttaa
     1921 tgaagtaata aataatacgc ttgttgtaga tggtggattt aaagaagaat atttaaatat
     1981 tgaaaaagag aatttaagag aaaagataca atcaataata aatgataaaa aggaatatgc
     2041 acaagataaa tgtattgagg ctatgtgtaa aggtgagaga tatagtgtaa gtgaatttgg
     2101 ttatgaagat gaaatagata gtataacttc aagagagtta tatgaacatt ataaaaatat
     2161 acttaaaaca tcacctatag atatagttgt agaaggtaat tttgatgaag ataaagttgt
     2221 agatataata agtaaaaatc taaaatttga aagagaagaa attataaata taccaagagc
     2281 agattttata aaaaatgtgg atgaggtaaa agttatagat gaacaaatgg aaataactca
     2341 aggtaagtta gttatgggtt atagagcaaa tgtggattat gcagatgtag ataaatatta
     2401 tgctttagta gttggaagca atgtcttagg tggtggtcca cactctaaat tatttgttaa
     2461 tgtaagagaa aaagaaagtt tatgttacta tatattctct tcaatagaga agtataaaag
     2521 tatcatgttt atatcatctg gtatagaaac taaagattat gataaagctg tagaattgat
     2581 aaaaaaacaa gtagaaagtg taaaagcagg tgatatatct gatgaagaat tagaaaacag
     2641 taaacttgct ttagttaatt ctatgaaatc tataactgat aatataggtg gtatgtctga
     2701 ttttgctttc tctcagtcta tggcaaagac aaattctgaa gtacaagaca taatatctag
     2761 tatagaaaag gttacaaaaa aagatatagt tgaagctata aaaaatatag agcttgatac
     2821 tgtatacttc ttgagaaatt aatggaggag atgtctatgg aaaaaatagt taatgatata
     2881 ttgaaagaag aagtttatta tgagaaattg caaaatggat taggtgtata ttttatgcct
     2941 aaaaggggtt ttatgaaaaa gtatgctatt ttggctacta attatggttc aaatgactta
     3001 gaatttgtac caattggaga agataagaaa attagagtga atgaaggtat tgcacatttt
     3061 ctagagcata agatgtttga gcaacctgat ggaggagatg catttgataa attttcaaaa
     3121 ttaggagtaa atgcaaatgc atttactaat tttacaatga cagcctactt attttctgct
     3181 actgaaaatt tttatgagag tttagaacat ctaattgact atgttcaaac accttatttt
     3241 acagatgaaa atgttgaaaa ggaaaaaggt ataatagcac aagagataaa aatgtacaat
     3301 gatgaccctg attggaatgt atactttaat tgcttgaagg caatgtatgt aaattatcct
     3361 gctagaattg atatagcagg tactgttgat agtatatata agataactaa ggaagagtta
     3421 tataaatgtt acaacacttt ctataatcca ggaaatatgg ctttatttgt tgttggtgac
     3481 ttagatgtag aaaaagttat agatgtaact aaaaagtcaa acaattacaa agtagacaaa
     3541 ctctctaaga gtatagaaag attttatcca gaagaaccag aaagtgtaaa agaaaaagag
     3601 gttatagaaa aattccctat atctatgcct atgttcaaca taggttttaa agatagtaat
     3661 gttggcttaa aagggaaaga acttttacgt aaagagattg taactgatat acttgtaggg
     3721 atgttattta aaaaaggaag taagctttat gaagatttgt atatgcaagg tttaataaat
     3781 gaaaattttg gagctggatt ttcgtctcaa gtggattatg cttttagcat aatagctgga
     3841 gattctaaag agcctaaaaa agtgaaagaa ataattcttg attatataga aaaatcaaaa
     3901 aaagaaggat tatcaaaaga agagtttgag agaactaaaa agaaaaaaat agggtcattt
     3961 attaagtgct ttgattctat aaactttata ggtaatagtt ttatatctta tgtttttaag
     4021 gatataaatc tattggatta tttagatgtt attaaagata ttacttttga ggaagttgaa
     4081 gaaagattaa aagaacattt taaagaagat tattgtgtta tttctattgt agagcctaaa
     4141 tagaaactta atcataataa gaataactaa ttaagtatta gtttatgttt ttaaatgttt
     4201 attttgataa aacaaattta taaaatatat ttgtataaat tgagctaaaa atttaagagt
     4261 catagataag gtatagaaga ggttgatgta tatatgcatc aatctttttg attaaaaaat
     4321 atctatgtac acttgtgact ttagtcatga gagattcagt ttaaaaaatt ttaaagtaat
     4381 tagattagta taatatagtg tagggattta tagaataaat tttaatattt tttgtagaat
     4441 taacattaat gtgttatcat aatttagtgt aattacaatt aattgtaatt atagcatatt
     4501 gctttggaaa tattttcaat taatacaaag aggtgacgtt atggatagag tagcatttac
     4561 actgtttggc atagacatca tgtggtatgg aatacttatg gcatgtggaa tgattttagg
     4621 aactttaata gcaataaaag aagcaaaaag agtgggaatt aaagaagatg atgtattaaa
     4681 tatagctata atagcaatcc cagttggact aatctgtgca agaatctatt atgttgtatt
     4741 taattggagt tactatgctc aaaatatgtc tcaaattttt aattttagag gtggtggact
     4801 tgctatacat ggagggttaa ttggtggaat attagctggt tatatataca ctaagattaa
     4861 aaatattaac ttcttaaaaa tggctgatac agttatactt ggaatgcctt tagcacaagc
     4921 tattggaaga tggggaaatt ttattaatgg agaagcacat ggaggtgcta cgaatttacc
     4981 ttggggaata atggttgatg gagtaaaggt acatccaact tttttatatg aatccatatg
     5041 ggattttgga atatttatag ttttattact atttagaaaa aataaaaaat atgaaggtca
     5101 agttatagtt acttatataa ctttatattc aataggaagg ttttttatag agggattaag
     5161 aacagatagt ttaatgttag gaccacttag aatggcacaa gttataagtc taattggagt
     5221 tattggtggt ataattgcac atgtttattt atcaaagaaa aataatatta gtgaagaata
     5281 gtttaaaaag tgaatttaag ggaggtaact atggaatttc atcatgtatc agttctttta
     5341 aatgagtgta tagagaattt aaatataaag ccagatggag tgtatgttga ctgtactatg
     5401 ggaggagcag gacattctaa agaaatagta aaaaaactgt cagataaggg attatttata
     5461 gggtttgacc aagataaaaa tgctataagc acagcaaaag agagattgag tgagtatgaa
     5521 agtagagtga agtttgttca cagcaatttt gaaaatataa aagaggaatt agaaaaaata
     5581 ggagtttaca aaattgatgg tgtattagct gatttaggcg tttcatctca tcaacttgat
     5641 gaagctgata gaggattttc ttatatgcaa gatgcaccac ttgatatgag aatggatgtt
     5701 aggtgtgagt tttctgcata tgatgttgta aatacttata cagaagatga attaactaag
     5761 ataataaaag attatggaga agataactgg gctaagcgta tagctaaatt tatagttgaa
     5821 gaaagagcaa acaaaccaat agaaactaca ggagaattag tagatgtaat taaaaaagct
     5881 atccctaaga aagctagaat agatggacct cacccagcta aaagaacttt tcaggcaata
     5941 cgaatagaag taaataatga acttggagta attacaaaga tgataaatga tgcttcttct
     6001 ataatgaatg aaggtggaag aatatgtata ataacattcc attcattaga agatagaata
     6061 gtgaaaaatg catttaagca tttagcatca gattgtatat gtccacaaca tttacctata
     6121 tgccaatgtg acaaagaatc tgaggtgaaa ataataacta gaaaacctat attacctagt
     6181 gaggaagaaa tagaagtaaa tccacgctca agaagtgcta agctaagagt agcagaaaaa
     6241 atataaatta taagactaaa gattaatgtt atatataaaa taaaattatt ataattaatt
     6301 aaaaagtatt atatattttg ttgcactggt aaaagtaatt aaaaaacaaa taacactata
     6361 agaaatgttc tttgttattg agacatataa tgaaaattta gatataaaaa tatagatata
     6421 gatgatatta agtaatataa tggagaactt aggaatcaga gagtagatag ttagttacat
     6481 attatattta tggaagaaat tatctaaaga tactagaagt gataaaaata aatagaaatt
     6541 aatatctaac ctatagattc gtataaaagc ataagtgaat ttataggttt ttgttgtgta
     6601 tagaaaaaaa tacaattaga ttgtgtaata gtgtattaat ttagcgatga aattttagag
     6661 gaagaaatat gattaataac aaatatagta tagaggaaaa cttaaactat atactatcgg
     6721 ggtgagaatt tgaaaaaaaa ttataaaata cataatctaa atgaatataa aaataaaaaa
     6781 aagaataatt ataaaaagag aaataagaaa aaaataggag ctaaatgcat tttactgatt
     6841 gcaacttttt cagtaataat attaaatcta tgtggttatt caaaaatctc agaaatgaag
     6901 tacgatatct attatttaca aaaagattta agaaaaaaag agatagtatt agaggaacta
     6961 aaggcaaaag aaactgaaaa aacatcaata aaagaaattg agctaaaggc taaggaattg
     7021 ctcaatatgg actatccaaa agatggacaa ataaagtata tagatgtgga taactagggg
     7081 gaatagattt gagaaaagta aagaggataa gtaagaaaag gctggtactc gttcttattc
     7141 tagcatgtgc attgtttttt tgccttgtta ttaggactgg atacctacaa cttatgaagg
     7201 gaaattggtt gagtacaaaa gcactagaac aacaaacaag agatatacca atagaaccca
     7261 aaagaggaac tatatatgat agaaatatga aagagttagc agtaagtgta actaagtata
     7321 ctgtttggtg taaacctgtg gaagtagaag ataaaaagga agcagccgaa aaggtagctg
     7381 agattttgga tgaagattat aaagatattt acgctttaat aagcaaaaaa aatatggctc
     7441 ttgtaaaagt aaaaagatgg atagatgatg ataaggcaag tcaaataaga gatgctaagt
     7501 taagtggaat atgggtagca gaagataatc aaagatatta cccatatggg aattttgcac
     7561 catatgtgtt aggacatact tcttcagatg caacaggtat atctggtgta gaaatgcaat
     7621 atgataaaaa gttaaaagga aagcctggaa aacttatagt aagtactgat gcctcaggaa
     7681 gagaaatacc acaaggaatg gaaaaatatt atgagcctgt acaaggtaat ggtcttgtgt
     7741 tgtctataga tgaagtaatt caacactata ctgaaaaagc agtacagaaa gcttatgagt
     7801 taaataatgc aaaaaaggtt actgcgatag ctatgaaccc taaaacaggt gatatacttg
     7861 ctttagcatc aaaaccagat tatgacccta atgactcaag aacgccaata tatccatatt
     7921 atcaagaaga gcttgaaaaa tataatgata aagataaaat aaaaggatat tatcaaatgt
     7981 ggagaaatcc agcagtaagt gatacatatg aaccaggttc tacatttaag cttattacat
     8041 cttccagtgc tcttgaagaa ggagtaatta aagatggaga aaaattcaca tgtacaggta
     8101 gtgtaactgt tggaggaaga aagattaaat gttggagaca ttacagacct catggaacac
     8161 aagagtttaa acaagcagta caaaactctt gcaacccagt atttgttgag ttaggaagca
     8221 gattaggggt tgggaaaatg tatgattata tagaatcatt tggtcttatg gataagacag
     8281 gtatagattt accaggagaa gctaagggaa tactgtacaa cgaaaaaaat gtaggaccag
     8341 ttgaattggc tacaatatca tttggtcaat ctatatcagt aactccaata caacttataa
     8401 cagctatatc atcaatagct aatggtggag atttaatgca accaagagta gtaaaatctt
     8461 atactgacaa taaaggaaat ataactgaaa cagtaaaacc aaaaaaagta agaagtgtta
     8521 tatctaaaga aacatctaaa aaaatgttag aaattgcaga atcagttgta actgaaggtg
     8581 gaggaaagat agcatatata ccagggtata gacttggtgg taaaacaggt acagctcaaa
     8641 aagttataga tggtaagtat gctccaggaa agtatatatg ttcatttgtt ggaatagcac
     8701 cttgtgatga cccgcaaata gtagttcttg ctattgttga cgaaccaaca ggagtaagtg
     8761 catttggtag tactacagca ggaccaatag tgaaagaaat aatgaatgac tctttaaaat
     8821 atttaggagt taaaccagta tataaagaag aagaaaaagc tgagtatgaa aagaagcaag
     8881 ttaaagtgcc agatgtaaga aatctaaaaa taggagatgc tgtaaaggca ttggaggatg
     8941 cgaagttaaa accagactta gatgcagata tagaacttcc agaagataca aaggtaaagg
     9001 atatattccc aaaacctgga gtaaaagtta atgaagactc aagtataacc ctatattttg
     9061 aaaactaaaa agtaacagat aattaacata tacccataaa catcttcaaa aaaacagaaa
     9121 tatagtatat aattataaag ttaagagtta tcgtaggcaa tgtatatata acggtataca
     9181 ttgccttttg ttgctaagag aatatagcta aggttttaaa ggaggagttt aataatggag
     9241 tgcttaacaa taaaagagtt agtacttgca actaatggga agttaatata tggagattat
     9301 aatgactgtg taagtgatat agttatagat agcagggagg caaatgccca aaatgcattt
     9361 gtagccatag ttggagaaaa tttagatggg catacattta tgaagtctgc atatgatagt
     9421 ggatgtaaaa cttttataaa aaatgaaagt aatggaataa aattagaaag ttcggatata
     9481 aatttaatag aggtaaaaga tacatctttg gctctaggag atatttcaaa atactataaa
     9541 gaaaaatttg atattccatt tataggtgtt acaggtagtg tgggaaaaac tactactaga
     9601 gatatgattt actctgccat atctgcaaaa ttaaatatac taaaaaatga aaaaaactta
     9661 aataatcatt ttggagttcc attgacttta tttaatctaa ataaagaaca tgagtgtgct
     9721 gtaatagaga tgggtatgtc tggattcaat gaaataaaat atttagtgga tatagtaaat
     9781 cctaaaattg cagtcatatc gaatatagga ttatcacatg tagaaaaact aggttcacaa
     9841 gagggaattt tgaaagcaaa gatggagatt acttcaaact ttgatgaaac aaatacttta
     9901 attgtaaatg gagatgataa gtttttatct acattaaaag aaaaagaaca tgtatataag
     9961 ttaaaaacat ttggctttaa taaaaacaat gacgtttatt gtgaaagtta tactatggaa
    10021 gaggatagtt taacatttat atgtgtaata aatgggaaaa aagaggaaat atttatacca
    10081 acaataggag aacataatat atataatgca atgtctgcta tattagttgg tttatgtcta
    10141 aatatttctt tagattatat aaaaaaaggt ttaaaaaatt ttaaagggac aaaaatgaga
    10201 ctagacataa taaaaaatga aaaattaaca ataattaatg attcatataa tgctagtcca
    10261 gattctatgg atgctgcttt aaagatactt ggaagatata aaggtagaag ggtagcgata
    10321 cttggagata tactagaaat gggtgaaatt tcagagtatg gtcatagatt agttggaaaa
    10381 tcatctatga ataatactga tattataata actataggtg aaaattcagt gtttattgga
    10441 gaagaagcaa aacaattagg attcaattca gccaatatat atcactttga gaatagagaa
    10501 gatgtgttta acaagttaaa tgaattagta aaaacaggag acactatttt agtaaaaggc
    10561 tcaagaggca tgagattaga aaaaatagtc gaatatctaa ataaataagc ttaaactaat
    10621 gagttaattg acaagggaga ggagttttat gatgttagga ataacagagc ttacgtacac
    10681 cgcattgatt gcgtttttaa tagtaattat aataggacca atatttatac caatgcttag
    10741 aaaatttaaa tttggtcaga cagttagaga tgatggaccc caaacacatt tagctaaaaa
    10801 cggaacacct actatgggtg gaattataat gattgttgca attctaataa ctggacttac
    10861 aagagtaaaa gttagtcatg atatggccgt aggtcttata tgtattgcag gttttggttt
    10921 tattggcttt ttagatgatt ttataaaaat aaagctaaaa agatcacttg gactaaaagc
    10981 atatcaaaaa ataatccttc aagttgcatt atctttttat gttgcttttt atcagtatac
    11041 atcatcatca agtgcttcac aacttatgat accatttaca gattttgtga taaatgttgg
    11101 aatattatat ataccaataa tgatgtttat aattgtagca atagtaaatg ctgtaaactt
    11161 gactgatgga ttagatggct tagcatcagg agtaacatta atagtttctg tattctttat
    11221 gctatttgca agttctattg ctggaaatac agaagtcgca gttttagcgg cagcaactgt
    11281 tggagcatgt ttaggttttt taggatttaa ttcatatccg gctagagtgt ttatgggcga
    11341 tacaggttca atggcattag gaggtgcagt tgtagcattt tcagttctaa ctaactcagt
    11401 gcttataata cctataattg gaggcattta ttttgcggag gctctatcag ttttgattca
    11461 agttggatat tttaaagcaa cgagaaagag attctttaag atggctccaa tacaccacca
    11521 ctttgaacag tgtggttggc cagaaacaag agttgtattt attttttgga ttataacagt
    11581 tgttttagct tggataagta taatagcagt attttagggc tagggggaat gaaaaatgga
    11641 cttaaaagga aaaaaagttt tattagtagg acttgcaaaa actggtatat ctacaattaa
    11701 acatttagat aaattgggtg caagtataat agtaaatgat atcaaagatg aaaataaatt
    11761 gagaaatata cttgatgaat tggaatctat aaatgacata aaatacatat taggacatca
    11821 tccagaagat gtagatgata tagatatggt tgtagtgtct ccaggtgtac cactagacct
    11881 accattcata ttgaaattaa aaaattcagg caagtatata attggtgagg tagaattagc
    11941 ttttaaacta tcaaataatc caatttttat agggattaca ggtacaaatg gaaagacaac
    12001 tacaactagt ttggtaggag aaattttttc aagagcaaaa cgagatacat atgtagttgg
    12061 taatatagga aatcctgtaa tagatactat agaaacttca agtgaagaat ctgtgttagt
    12121 aactgaacta agcagttttc aacttgagag tatagatgaa tttagaccta aagtaagcgc
    12181 aatattaaat attacagagg accacttaaa tagacaccat actatggaaa aatacataga
    12241 agcaaaggca aatatattta tgaatcaaac tgttgaggat ttctgtatat taaactatga
    12301 tgatgagata gtaaaatctt tagctgataa atgcaatgcc aaagtaattt acttttcaag
    12361 aactaaaaaa gtaaatggag gagtatattt agaaaataat gacataataa tagatataga
    12421 tgataaaatc aaatttctaa ataaagatga tgtaagttta cctggaggtc ataacttaga
    12481 aaactgtatg gcagctatag caatagcata tgtctgcaaa attgatttag aagttataag
    12541 agatgtactt atgacattta aaggagtgga acacagacaa gagtttgtaa gaaacttaga
    12601 caatgtaata tatgtaaatg attcaaaggg aactaatcct gattctacta taaaagctat
    12661 acagtcttac gatagaccta taatacttat agcaggaggt atggataagg gaagtaattt
    12721 tgatgaactt cttgaaactg caaagtcata tgttaaatca ttagtcttat taggagaaac
    12781 tgcatcaaat atagaaaatt gtgctaaaaa taaaggattt aatgacatac atatagtaaa
    12841 agatatggaa gaagcagtta agacttctta tgaaatttca aaaagtggag atatagtgct
    12901 tctttcacca gcttgtgcaa gttgggatat gtatgaaagt tttgaagtaa gaggaaaaga
    12961 cttcaaagat aatgtaaaca atttaaaata atctcttacc tctaaactta cttaaatgga
    13021 agaatggagg tagattagtt ggatgcctaa agaaaatttg aaaaaacaaa ttgacataag
    13081 gatgaagact gaatttgatg gagtagtgtt ttacactact atgcttttag tatttgtagg
    13141 aatagttatg gtgtttagtg caagttttat acaatcttca tttaaacaca atgatgcgta
    13201 ctattttcta aaaaagaatg taatatatgc tgtacttgga tttatagtta tgattattac
    13261 atcgagaata gattatagtt tttggaagaa aaatgctaca gcaattggag ctatagcagt
    13321 agtattatta ttgttagttt tgaccccttt ggggatagaa gcaaatggag caaaaagatg
    13381 gttgggaata ggagctttaa catttcaacc tgcagaaatc gcgaagtttg ctacaataat
    13441 attaactgca aaattaattg aaaaaaatta tgataaaata aaatctttga caaaaggtgt
    13501 tgtaccactt ctcgtagttc cagggatatt ttttgcgttg ataatacttc agccaaattt
    13561 gtcaacagca ggtactgtaa ttctagttac ttttgtaatg atatttgttg ctggaatgga
    13621 tatgaaaatt gtatttgcta tgatagggag tggggctgct ttatttgctg cattagtaat
    13681 tgctgaaccc tatagactta gtcgtgttac atcattttta gacccttttc aagaccctct
    13741 tggtaagggg tatcaggtaa tacaaggtct atatgcacta ggttcaggtg gattgtttgg
    13801 tctgggctta ggaaagagta aacaaaagta tttttatata ccagagccac aaaatgactt
    13861 tatatttgct ataataggag aagagttagg attgattggt tgtataatag ttattatgct
    13921 atttgtagtt ttagtctaca gatgtgtaag aatagctttg aagacttcta atgtatttgc
    13981 ttgtatggtt gttataggta taggtgcaca aattggaata caagcagctt taaatatagc
    14041 agttgcgaca tcatcaatgc cagctacagg tgtagcacta ccatttataa gttatggagg
    14101 aacttcactc accatcttta tgggagctgt aggaatagtg ttaaacatct caaagcatgt
    14161 caaaattaat taacaggaga tgatgatttg atgaaagttt tattatcagg cggtggaact
    14221 ggagggcacg tgtatccagc tatagctatt gcaaacaaaa taagagatga gcatccagac
    14281 gctgagataa tttttgttgg gacggaaaaa ggaatagagt cagaaatagt tcctaaatat
    14341 ggatttgaat taaagactgt aacagttcaa ggtttcaagc gaaaaataga tttcgataat
    14401 gtaaaaagag tttttaagct ttttaaaggt ttggagcaat caagaaagat tgttaagaaa
    14461 tttaagccag atatagttat aggaactggt ggatatgtta gtggacctgt tttatttaat
    14521 gcatctatgg gaaaaatacc agccataata catgagcaaa attcttttcc tggtgtaact
    14581 aataagattt tatctaaaac agttacaaag gtacttacaa gctttgaaga ttcacataaa
    14641 agatttcctg aggcagcaga agaaaaatta gtttttacag gaaacccagt cagaaaagaa
    14701 attttactat caagaaaaaa tattgcaaga aaaaatctat caataagtga cgaaaaaaga
    14761 atggtacttt gctatggtgg tagtggtggt tcaagaaaaa taaatgatgc aatgagactt
    14821 gtaataaaga atatggtaaa tgaagatata gcttttatat ttgctacagg caaaagctat
    14881 tatgatgaat ttatggaaag tattagcgat ataaatctta aaccatacca aaaagttgtg
    14941 ccttacttag aagatatggc aaatgcactt gcagcaagtg atttagttat aggtagcgca
    15001 ggagcaattt cattagctga gataacggct cttggaaagc catctataat aatacctaaa
    15061 gcttatactg ctgaaaatca tcaagagtat aatgccaaga gtatagaaaa acaaggggca
    15121 ggaattgcta ttttagaaaa aaatctaact ccagagagcc taaatacagc agtatttaaa
    15181 cttttaggag atagagaact tttggttgat atggcaaatg caagtaagac tataggtaag
    15241 cctgaagcca tagaccttat atatgatgag ataatgaaag tatacaattc aacacaaaag
    15301 tcaacttcta aaaaaacaaa aaaagaaaaa gttattaagg aagtaaaaga aataaaaaaa
    15361 gaaacgactc caagtataga aggtcaagct aaggtaatag gaataaagaa aagataagta
    15421 aatatttacc cctaatttat aatatattag attttctaa
//
LOCUS       DAEMAE010000084        15334 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEMAE010000084 DAEMAE010000000
VERSION     DAEMAE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.086"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2426
                     /gene="priA"
                     /locus_tag="KPH93_002010"
     CDS             <1..2426
                     /gene="priA"
                     /locus_tag="KPH93_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG1688830.1"
                     /translation="QIPVFLSDVIKIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKI
                     KEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECINLIYPKGYKLNNYKVVLLGESL
                     SGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIK
                     LCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKINVGSKQKEILSFLKNNENVEIND
                     LLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIYKSVLKRIKLNNEQQEAVDEIKS
                     NMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSR
                     FGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGARSALFAPFNSLGLIIIDECHESS
                     YKSEKNPKFNAIEVARFMALKNNITLILGSATPSIEEYYRAKSGEYKLINIKSRANDK
                     PLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEENNQVILFLNRRGYANFVSCRKC
                     GYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEE
                     LKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEVDVLIGTQMLSKGLDFENVTLVG
                     ILSADMILNFPDFKSFETTFQLITQVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYD
                     YEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLVIKNIKNMHASLIYLLEKRGIND
                     LGFILGPNPCSISKINQNYRWQILFKDENIEINLLKGIIKYICITKRDLIFDKNINVS
                     IDINPNSVL"
     gene            2444..2884
                     /gene="def"
                     /locus_tag="KPH93_002011"
     CDS             2444..2884
                     /gene="def"
                     /locus_tag="KPH93_002011"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG1688831.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2899..3828
                     /locus_tag="KPH93_002012"
     CDS             2899..3828
                     /locus_tag="KPH93_002012"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG1688832.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3838..4590
                     /locus_tag="KPH93_002013"
     CDS             3838..4590
                     /locus_tag="KPH93_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG1688833.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKCVNSNIMKINFNIVSGLFPIISFIASSFGMSKSEIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            4659..5360
                     /locus_tag="KPH93_002014"
     CDS             4659..5360
                     /locus_tag="KPH93_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG1688834.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            5422..6747
                     /gene="rsmB"
                     /locus_tag="KPH93_002015"
     CDS             5422..6747
                     /gene="rsmB"
                     /locus_tag="KPH93_002015"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG1688835.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLIKSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            6755..7786
                     /gene="rlmN"
                     /locus_tag="KPH93_002016"
     CDS             6755..7786
                     /gene="rlmN"
                     /locus_tag="KPH93_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG1688836.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            7806..8558
                     /locus_tag="KPH93_002017"
     CDS             7806..8558
                     /locus_tag="KPH93_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG1688837.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            8575..10590
                     /gene="pknB"
                     /locus_tag="KPH93_002018"
     CDS             8575..10590
                     /gene="pknB"
                     /locus_tag="KPH93_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG1688838.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPALEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLNQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPDSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSPGTGANIGKTE"
     gene            10698..11435
                     /locus_tag="KPH93_002019"
     CDS             10698..11435
                     /locus_tag="KPH93_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG1688839.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENGAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDVLVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            11533..12417
                     /gene="rsgA"
                     /locus_tag="KPH93_002020"
     CDS             11533..12417
                     /gene="rsgA"
                     /locus_tag="KPH93_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG1688840.1"
                     /translation="MLEGKIIKGISGFYYVDTDNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEVDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVANGEISKERY
                     DSYIQLLNEIRQNNSRRY"
     gene            12422..13093
                     /locus_tag="KPH93_002021"
     CDS             12422..13093
                     /locus_tag="KPH93_002021"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG1688841.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            13102..13743
                     /locus_tag="KPH93_002022"
     CDS             13102..13743
                     /locus_tag="KPH93_002022"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG1688842.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKVNHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            13874..14503
                     /locus_tag="KPH93_002023"
     CDS             13874..14503
                     /locus_tag="KPH93_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG1688843.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLDECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            14558..15214
                     /locus_tag="KPH93_002024"
     CDS             14558..15214
                     /locus_tag="KPH93_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1688844.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVLDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         6183 a         1529 c         2967 g         4655 t
ORIGIN      
        1 atcaaattcc tgtattttta tctgatgtaa taaaaatcgg acacagaata ttagtaccat
       61 ttggaaaagg taataaacca acagaagcct ttgtatttca atttacagat agcttagatg
      121 aaaaaataaa aataaaagaa attattgata tattagatga aaatccaata tttagaaaag
      181 aagatttaga gttggtttat tggatgaaaa atagatattt gtgtacttat atagaatgta
      241 taaatttgat atatccaaag ggatacaaac taaataatta taaagtagtc ctattaggtg
      301 aaagtttaag tggattaaat gatattgaat tgaaggaaaa aatatcaact ttaagtgata
      361 aaaatagaga aatagtagag aaagtgattg atagtaaagg taaaattaag gttgataagt
      421 taaaatatat accaaactta aatagttctt tgtacacaat gaataaaaat ggaattataa
      481 aattatgttg ggaatataaa aatcacaaaa atgaaaagaa agtatgttat atatctttga
      541 gtttagaaag tgataagatt gacgattata tagagcaaaa taaaataaat gtgggaagta
      601 aacaaaaaga gatattaagt tttcttaaaa ataatgaaaa tgtagagata aatgatttat
      661 tggatttatt aaatgcttca aaacaaagta taaattctct aagcaagaaa aagctaataa
      721 ccctagaatt taaggattat tatagagagc caaaaagtat ttacaaatcc gtattaaaaa
      781 ggataaaatt aaacaatgag caacaagaag cagttgatga gataaagtcg aatatgtttg
      841 tagatgataa aaaaccatac ttaattcatg gagtaactgg aagtggaaaa actgaagttt
      901 acatggaaat aatagaattt gctctaaatc aaggcttaga cagtatattc ttagtacctg
      961 agatagcatt aactcctcaa acaatagata ggctaaagag tagatttgga gatttagttg
     1021 gagtgttcca cagtaaacta tctgaaggag aaaaacatga tgtttacaag gctgtcaaag
     1081 caggcaaagt aagagtgtta attggagcta ggtcagcttt atttgcacct tttaattctt
     1141 taggcttaat aataattgat gaatgtcatg agtcatcata taagtctgag aagaatccaa
     1201 aatttaatgc tatagaagtt gctagattta tggcattaaa aaataatata actcttattt
     1261 taggttcagc aacaccctca attgaagagt attatagggc aaaatctgga gaatacaaac
     1321 ttataaatat aaaaagtaga gcaaatgata agcctcttcc aaacattgaa gtggtagaca
     1381 tgaaagatga gttagataaa ggaaatagaa gtatatttag tttgaaatta caaaaagaaa
     1441 tacgttatgc gattgaagaa aataatcaag ttatactatt tttaaataga agaggttatg
     1501 caaattttgt atcttgtaga aagtgtggat atgtgtttca atgtgaaaat tgtgatatat
     1561 ctttaactta tcataagaaa agcaatactg gaagatgcca ctattgtgga tatgagaaag
     1621 aaattccaaa agagtgtcct gaatgtaaaa gtacttatgt taagccattt ggagttggaa
     1681 ctcaaaaaat tgaagaggaa ttaaaatata tttttccaga tataaaaact ttgagaatgg
     1741 ataaagatac aacttcaaaa aaaggtgctt tagatgagat actaaataaa tttaaagata
     1801 aagaagtaga tgtactgatt ggaacacaga tgttaagtaa agggttggat tttgagaatg
     1861 taactttagt aggtattttg tctgcagaca tgatattaaa ttttccagac tttaaaagtt
     1921 ttgaaactac atttcaattg ataactcaag tctctggaag agcagggaga gctgataaag
     1981 agggaaaagt agtacttcag acttatgaca ctgaacacta tgctattaag catgctattg
     2041 aatatgatta tgagggtttc tatgaagatg agataaaaat aagaaaagca tttggatact
     2101 ctccttttaa caacatgtta agtgtagttg ttagtggtga agatgaaaga ttagtgataa
     2161 aaaatataaa aaatatgcat gcatcattga tttatttatt agaaaaaagg ggtataaatg
     2221 atttaggatt tatactaggt ccaaatccat gttcaatatc aaaaataaat caaaattata
     2281 gatggcaaat actctttaaa gatgaaaata ttgaaattaa tctcttaaag ggtataataa
     2341 aatatatatg tataacaaaa cgagatttga tatttgataa gaatatcaat gtatctatag
     2401 atattaatcc aaacagtgta ttataaattt aggaggaatt ataatggctt taagacaaat
     2461 agttcaaata ggagaaccag tattaagaaa aaaatcaaaa aaggttgaaa aaattgatga
     2521 aaagataata caattattag atgatatggc agaaactatg tatgatgcag atggtgttgg
     2581 actggctgca cctcaagttg gaatattaaa aagagtagta gtaatagata ttggagaaga
     2641 gcttatagaa cttataaatc ctgaaataat agaaacatct ggggagcaaa tagatgagga
     2701 aggatgctta agtgttgttg gagaagcggg aaatgttaga agaccaaatt atgtaaaggt
     2761 tagagcttta aatagaaatg gtgaaacaat agagttagag ggagaagaac ttttagctag
     2821 agcattttgt catgagatag accacttaga tggaatttta tttgttgaca aaatagaaaa
     2881 gtagtaggag ttgatttgat gaaaatagta tttatgggaa ctcctgatat agcagttcca
     2941 tgtttacaaa aaataataga tgagaagtat gaaatattgg gtgttgtaac tcaaccagat
     3001 aaaccaaaag gaagaggtaa aaaactaggt atgagcccag taaaagaact ggcaattgaa
     3061 aataatatac ctgtatatca accagtaaag gctagagata aagaatttat agataaaatt
     3121 aaatctttaa atccagatgt aatagtagtt gtagcttttg gacagatact tccaaaagaa
     3181 atattagaga ttcctaaact tggatgtata aatgttcatg tttctttact tccaaaatat
     3241 agaggtgcgg cacctataaa ttgggtaata ataaatggtg aagaaaagac tggtgttaca
     3301 actatgtata tggatgaagg attagatact ggagatatga tactaaagac agaagtaaat
     3361 cttgatgaaa atataacagc aggagaactt catgataaaa tgatgaatat aggagcagaa
     3421 actttaaaag aaacattaag attaattgaa gaaggaaatg ctccaagaga agtacaaaat
     3481 catgaagaat tttcctatgc gccaataatg aataaatcat taggtaacat agatttttca
     3541 aaaagtgcca gagaaataca caatttagtt agaggagtaa atccatggcc aagtgcttat
     3601 actacctata atgatgtcat aatgaaagtt tggaaaacta aagtgttaga tgaaaaaagc
     3661 acaaaagatg taggtacaat aattgatgta agcaaagatg ggataaaagt aagtactata
     3721 gataatgtac ttttaattga agaaattcag atgccgaata aaaagagaat gttggttggt
     3781 gaatatataa aagggaatac tatagaaact ggtttagtat tgggttaggt gaaaagtatg
     3841 acagatataa agaagtattt aacaagtgtt gggattttga ttttaatatt aggaattttg
     3901 ataggagttg ttaatttttt tataataagt ttaactcaaa tattctcctt gatgataaat
     3961 attataataa tatcactaat acttataata ctatgttcaa cagcagttac ttatagagta
     4021 attaaaggaa agtgtgttaa ctcaaatata atgaaaataa attttaatat tgtcagtgga
     4081 ttatttccca ttatatcttt cattgcatca tcatttggta tgtcaaaaag tgagattaga
     4141 agaatatata taaagttaaa taatgaatat atatacagta ataaatataa ttttaatcca
     4201 gaggatataa taattttaat acctcattgt attcaagaga atagttgtaa attaaaagtt
     4261 actaatgata tagataattg taaagaatgt ggaagatgca acgtaggaga attaattaag
     4321 ctaaaggaaa aaactaatgt aaaaatattt gtagctacag gaggaaccct agctagaaaa
     4381 attataatgg ataataaacc taaagctgtt gtagctgttg catgtgaaag agatttgaca
     4441 tctggaatac aagatattaa aaaaattcct gttttaggtg tatttaacaa aagacctaat
     4501 ggaccttgtg taaatactaa tgtagatatg atggatattg aaaaagctat aggcttttta
     4561 acaggaaaaa atatattagt ttgtaattaa actgttttta agttaagtga atattaagag
     4621 gatgtataat aaaaatatat tttaggagag tgttatttat gtaccctgta tatggcggtt
     4681 tttggggatt tgacccaaca atggttgtat taattccagc tatattactt actatatatg
     4741 cacaatttaa agtgagttca acaacaaata agtatttaag agtaaataca cgtcgaggat
     4801 atactggaga gcaaacagca agaagagtac ttgattcaaa tggtttgtac gatgtaaaga
     4861 tagaaatggt tagagggcat ctaagtgacc attatgaccc aagaagaaag gctgtaaggt
     4921 tatcagaaga tgtatattat ggaacatcta taacttctgt tgcagtagct gcacatgagt
     4981 gtggtcatgc aatacaacat gctaaaggtt atgcacctct tcaaataaga agtagtttag
     5041 taccagtagt gaattttgct tcaagtatat cttggttttt aatatttcta ggatttatta
     5101 tggctggtcc atttttaaaa attggtatac tattgttttc ggcttcagtg ttattccaaa
     5161 taatcacatt gcctgtagaa tttaatgctt caagtagagc aatcgttcaa cttggaaatt
     5221 taggtattat agatgagagt gagtcaagac aaagtagaag agtactttca gcagcagctt
     5281 taacatatgt tgcagcagca ttggtttcaa tacttcaatt acttagattg ttactaatag
     5341 ctcaaagaag aaatgactaa aagtaaagtg ggttgtttag aaacaaccct tttttatgtt
     5401 taataaaagg gagaaattta tatggatgca agagaagttg gatttaaagt attatgtgat
     5461 atagaaaaaa atgacaatta ttcaaatatt gcaataaata agcattttaa aaatttagaa
     5521 ataagtgata tggatagagg acttgcaaca gagttaattt atggagtaat agaaaataaa
     5581 tattatttag attatataat caataaactt tcaaaaataa aggtaaaaaa aatgtcaact
     5641 tatgttaaaa tctttttaag aatgggaaca tatcagattt tatttttaga tagcatatca
     5701 gattatgcag cagttaatga aactgtaaaa ttatctaaga aatatgataa aaaatcttct
     5761 ggtttcataa atgctattct tagaaatgaa ataagagcta aagagactat aatggacata
     5821 actgaggaag atagtgtaaa atacttatct ataaagtatt cctataactc ttggattata
     5881 aaaaattgga tagataaatt tggacaagaa tttgctgaag atttgctgga agcgaataat
     5941 gaaaagccta gtatctatat aagaacgaat acacttaaaa taagtagaga agaacttatt
     6001 gaaaaattaa gtgaaatggg tattgtatgt ttaaaagttc ctatggtaga agaagctatt
     6061 aaagtcgaaa aaatgaaaaa tatagaaaaa aatgaattat ttaaggctgg tttatttaca
     6121 attcaagata taagttctat gatagtaggg aaagtaatta atccaaaaga aggttcattt
     6181 gttttggatg tatgtagtgc tcctggaggg aaatctactc acttagctac tttaatgaac
     6241 aatactggtc aagtagtagc tagagatatt tttgagcata aactaaagct tataaagtct
     6301 actgtaaata gattggggct taaaaatgta tttgtagaaa gatttgatgc ttctgagatt
     6361 gatgaaaata gtataagtaa atttgattat gttttagcag atgttccttg ctctggtttt
     6421 ggtataataa gacgtaaacc agaaattaag tataagaaag aagaagagtt aaaagacatc
     6481 acttctatac aaaagaaaat tttagaaaat gcatctaaat atgtcaaagt tggtggaact
     6541 ttagtatata gtacatgtac aattcaagat gtggagaata taaatatagt tacttctttt
     6601 gttgaagaaa atcataactt tgaatttgtt ccgattgata cagtaaatgt agacttagac
     6661 aatcaagata aaggatattt aaagatatat cctaatattc atggtataga tggttttttc
     6721 atagcaaaat taaagagaat aaggtagtga gaaaatggaa aagaaaaaga tagtattaaa
     6781 aaattttact gaagatgaac ttaaagaatt tatgaaaact atagatgaaa agccctttag
     6841 aggaagtcaa atattctctt ggatatataa gggtgcaaaa acttttgatg atatgaataa
     6901 tataccaaaa agtttgagaa ataaattaga agaagtttca tgtatagggc atatagatat
     6961 agaattaaag ttagaatcta aggtagataa tacaaaaaaa tatttgtttt tattagatga
     7021 tggaaatata atagaaactg tgatgatgga ttatgatagt agagttactg tctgtgtttc
     7081 taaccaagta ggttgtagga tgggatgtaa tttttgtgca tctacaatgg atggattaat
     7141 tagaaactta gagccatggg aaattttaga ccaagttatt aaaattcaag aagatactgg
     7201 taagagggta tcaaatcttg tattgatggg gagtggtgag ccacttgata actttgaaaa
     7261 tacaaaacaa tttttaaaaa taattaacga aaaaaatggt cttaatatag ggtatagaca
     7321 tataactctt tcaacttgtg gaatagtacc taaaatgtat gagttggcag atttagaaat
     7381 agctataaat ttagctttat cacttcattc accatatgat gaagaacgaa gaaaaattat
     7441 gcctgtagca aatgcttatt ctattgaaga aatattgaat gcatgtagat attatataaa
     7501 aaagacaaat agaagggtta catttgaata ttctcttata aaaggggtaa atgactcaga
     7561 aaaagaagca aaggcattag ctaagctcct aaaaggtatg ctatgtcatg taaatttaat
     7621 tccaataaat aaagttgaag aaagagaata cgaaaagcca gataaagcat ttatttataa
     7681 atttagggat agtttggaga agaacaatat acctgccaca gttaggatgt ctatgggttc
     7741 tgatattagt ggagcttgtg ggcaattgag aagaaaatac aagtaatttg taaggaggaa
     7801 gtcgtatggt ttatagttgt gcctcccata taggaaaaat aagaaaaaac aatgaagact
     7861 attgtgaggg agaagttata gatacagaac atggtccaat aggaatattt gccatagctg
     7921 atggaatggg tggacataaa aaaggtgaag tcgccagtaa attagcagta gaaaatataa
     7981 ttgattttct aaaggaaaac ttattacaac atgataatgt aaaaatagac tatatagatg
     8041 atatattaaa acaggcttat aataatgtaa actcaattgt gcacaaaaaa tctatggaag
     8101 atatagaatt tgaaggaatg ggaactacat tagttacggc tatagtgtat aataatgtct
     8161 tatatgtggc aaatgttgga gatagcagat gttatttgtt aacagatgaa aaatttgata
     8221 aaataactat agaccattcc gtggttgaag aacttatgat ggcccatgtc attacagaag
     8281 aagaggcaag aagacatcca cagaggaata gaataactag agcaattgga acagatgata
     8341 tggttgttgt agatattttt aagaaagaaa tcaaaaaaag tgatataata cttcttgcga
     8401 cagatggatt aactgggttt attgatgatg aagatataag agatttaata ttagattatg
     8461 aatatgagag aactagtaat ataagtgaag aattgataag tatggccaat gatgtttctg
     8521 gtaaagataa tgtttcggtt atagtgataa aagtataatt aggacggtga tattgtggga
     8581 gatacaattt taggaaatcg atatgaaatc atcaggaaga ttggtgatgg aggaatggcc
     8641 tttgtatatg aggctaaaga cagattatta aacaggactg ttgcgcttaa agtacttaga
     8701 cctgaatttg tagatgatga tgaatttcta acaaagttta aaagagaggc agaagcagta
     8761 gcaagtcttt cacatccaaa tatagtaaat gtttatgatg ttggtgaaga tggaaaggtt
     8821 cactacattg taatggagtt tgtagatggg aagaatctaa aagagattat acaagatgaa
     8881 ggtatattag atgaatatac tgcattagat ataacaaaac aaatagctat ggcacttagt
     8941 gccgcgcaca aaaaggggat tatacataga gatataaaac ctcataatat cctcatatcg
     9001 aatgaaggaa gagtagtaaa agtggctgac tttggtatag ccaaagctgt ttcaaattca
     9061 acaatgacta atattggtag tataataggt tcagtgcatt atttttcacc agagcaagct
     9121 aaaggtaaat ttgtaactaa taatgctgac ctgtattctc taggtatagt tttatatgaa
     9181 atgttgatag gaaaagttcc atttagggga gatagtccaa tttctatagc acttcaacac
     9241 attaatgatg atatagactt tacatcagaa gaaaaagtta ggattcctca aagtgtaaga
     9301 actacaataa aaaagcttac agaaaaatct agtgcagata gatatcaaac tgctgaagaa
     9361 ttgatagaag atatagagta tatagaaaaa aatattgatt tagattttat aaaagaatat
     9421 gatgattttg caactaagaa aattgatgaa aaggaaatta ataaagttgt aaatccaaca
     9481 cttgcaaaac cagctcttga aaaagtggtt aagcctgttg aagtagctga tttggatgat
     9541 gatgaagatt actatgatga tttttatgaa gaagatgatg atgaagatga agaagaggaa
     9601 gagataatga gagctaaaaa gaatcagaga cctaagagta ctccaagtaa gagaactaag
     9661 aaaaagaaga aaaaacagga aagcccaaaa tctagaagaa gattaaaagt aattgcggct
     9721 gttttgatac tgatattgtg tgctcaagta ttcttagcat ataagttttt atttgctgga
     9781 gggtttggca ataaatcttt gactgttcct aatcttgtaa atatgacact agaagaagct
     9841 caaagcgctg ttgaaaaaga aggattatac ctgagtgtaa aaagtgaaga gtataatagt
     9901 gaagttgatg aaaattgtat aatatcacag acacctgaag gtggaagtac aaatgtcaaa
     9961 aagggtgata ccataaatgt tgttgtaagt aaaggttcaa gtcaggcatc tgttcctaat
    10021 gtggtgggac ttactttgag taatgcaaaa caacttattg aagaaaataa cttaaaagtg
    10081 ggaactgtta aatatgaata tagctcaata tataaagaag gtacagtttt aaatcagagt
    10141 ccaggtgctg gctcatcaag agctcaagaa ggtgatgagg taaacctata tgtaagcaaa
    10201 ggttcagaaa aatcaaatac acagactcca acagtaccag ataaaaaacc aacgacacca
    10261 gagaataata caccaacaga acctgattca aactcaggga acagtggtgg aaattcagga
    10321 agctcaaatt caggaaatag tggaggaaac tcaggcaata atggaggaaa ctcaggaggt
    10381 tcaaactcag gaaatagtgg aggaagctca ggaggctcaa actcaggaga caatggagga
    10441 agttcaggag gttcaaactc aggagataat ggaggaagtt caggaggctc aaactcagga
    10501 gacaatggag atagctcagg aggctcaaac tcaggagata atggaggaac ttcaccagga
    10561 actggcgcaa acattggtaa aacagaataa atattaataa aataagagct gtccttttat
    10621 agaaaatatt ttctttaaag ggcagtttgt tttgtataaa atattgaatt ggttataata
    10681 taaggagaaa agtatttatg aagaagaaaa cagttttaat tacaggagga gctagaggga
    10741 ttggtaaagc tatgtctaaa gcttttgcaa aagaaggtta caatgtatta gtaaatttta
    10801 ataagtcaga aaatggagct aaagagttgt atactatatt aaatgaaaaa aatttttctg
    10861 taaaactttt taaggctgat atttcaaata gagaagatgt agaggatatg gtagattatt
    10921 gtataaaaga atttggaggg ttggatgtac ttgtaaacaa tgctggtgta agtcaagaca
    10981 agttgtttac agatataact gatgaagatt gggataatat gatgaatatt aatttaaaag
    11041 gaagctttta ttgttcacaa gttgctttaa aatacatgat atcagagaaa aaaggaaata
    11101 taattaatat atcttcaatc tggggtatat caggtgcctc ttgtgaagtg cattattcta
    11161 taacaaaagc aggtataata ggcatgacaa aggcattagc taaagaagta ggaccatcaa
    11221 atattagagt aaatagcata gctccaggtg tcattaatac tgacatgtta tctggatata
    11281 atgaggaaga tatagacgtc cttgtggaag aaacccctct aatgaggcta ggaactccag
    11341 aagatatagc aaattgtgct attttcttgg catcagacaa atcaaatttt atcactggtc
    11401 aggtaattag tccaaacgga ggatttgtta tttaaaaaaa atgtggtatt atataaaaat
    11461 aggaaagaat aacacgatat actactcata aacagtatgt gtttatataa aaattataaa
    11521 gggaggcatt tgatgctaga aggaaaaatt ataaagggga ttagtggatt ttactatgta
    11581 gatacagata atggtattta tgaatgtaag gctagaggaa tatttagaaa acagaaaata
    11641 acacctctag ttggtgacag agtcaaaata agtattgttg atgaagatga aaaaaaaggt
    11701 atcttagaag aagtagacag tagagataca gagcttataa gacctcctat agcaaatgta
    11761 gataaggcat taatagtatt tgcaataaaa aatcctaaac caaacttatc tcttttagat
    11821 agatttatag ttttggcaga aaaagaaaat ttagaaactg taataatcct tacaaaagca
    11881 gatttagatg ataatgatac attagaaact gttaaaaata tatatgaact tagtggctat
    11941 aaagtcattc ctgtaagcaa tattactaag ttaaatatag ataaagtaaa agaagaatta
    12001 aaagaaaatg tagtagtgtt tgcaggacca tctggagttg gaaaatcaag cttactaaat
    12061 gaaatagatg aaaattttaa acttcaaaca ggagtggtaa gtgataaaat aaaaagggga
    12121 aaacatacta ctcgtcacgc tgaactttta aaattggaat ttggtggaat ggttgcagat
    12181 acaccaggtt ttagttccct tgcacttgaa gatatagagg aagttgaact taaagattat
    12241 tttatagaat ttgataaatt taatgattgt aagtttggtt caaagtgtat tcatgaaaat
    12301 gaacctaatt gtgctataaa agaagctgtt gcaaatggag aaatatctaa agaaagatat
    12361 gatagttata tacagctact aaatgaaata agacaaaata acagtaggag gtattagaaa
    12421 catgattaaa ttagcaccat caatattatc agcagatttt gcaaaattat tagaggatgt
    12481 aagaaaagta gaaagtgcag gatgtgaata tcttcatatc gatgtaatgg atggacactt
    12541 tgtaccaaac attacattag gaccacttgt agtcaaaagt ttaaagaaag aaaatataaa
    12601 tatggtattt gatgctcatc ttatgataga aaatcccgac cagtatattg aagaatttgt
    12661 aaaagcagga tgtgatataa taactgttca ccaggaggct tgtgttcatc tacatagaac
    12721 tatacaaaat ataaaatcac atggaataaa agctggtgta gtacttaatc cagctacacc
    12781 agtagatacg attaaacatg tactacctga tttagatatg gtgttattaa tgtctgttaa
    12841 ccctggattt ggaggacaat catttattcc ttgtgtttta gataagatta aagagttaaa
    12901 agctattata gatagtcaag gattaaacat tgatatagaa gttgatggtg gtataagccc
    12961 taaaaacgta gccgaagttg tacaagcagg agctaatgta atagtagcag gttctgcaat
    13021 atttggaagt gatgacattc aagaaacagt taatttattt agaaagaatg cttctcttga
    13081 agaattagtt taaatttaat aatgaaaatc tgtattgttt taaatggcga aattgaagat
    13141 tacgaagtta caagagatat tataattaag gaatgttatg actgcataat atgtgcagat
    13201 ggaggggcaa atcatactta taaaatggaa ataatgccag attatatatt aggagattta
    13261 gattcagtag aagaagaaaa gataaacttc tataaaaata aaggtgttaa atttgaaaaa
    13321 tttccatcta aaaaagatga gactgataca gagctttgtc tttttttagc taagacactt
    13381 aaagtaaatc atattgattt ttttggtgct ttagggggaa ggattgacca cactcttgca
    13441 aatataaagc tattatatta tttaaaagaa gatggtatat attctaggat actttctgat
    13501 aaagaagaga tgtatatagt tgaaaatgag gaaatttcat tgtatggaaa cccaggggac
    13561 actatttctg taatagctat aaatggtgat gctaagggtg taacacttac tggcttagaa
    13621 taccctctcg ataattatta tatgaaatac tctgtaccta ttggaatatc aaatgttatg
    13681 ttgagtaatt cttgtaagat taaagtagag caaggctgtg tacttgtagt tagaaattta
    13741 taaaatttga ttttgacaat tgattttata ttgtagagca gatacaatca agtaaaatat
    13801 atttatgatt gtatctgctt tttagtatat aattataaat atataataaa ctttgacata
    13861 agggggattt tttgtggtag atagtattat ttttgattta gatggaacat tgtgggattc
    13921 aactgaagaa gtatgcaaag tctggcagga tgttttaagt aaacataagg aaatagaatt
    13981 aagtgtcaca aaagatttat ttagaagtct tatggggctt tcttttgagg aaatagcaaa
    14041 tcgtttattt ccaaatttaa gtgaagaaga aagaatgaat ttattggatg aatgttcttt
    14101 gaaagaatgt gactatttat ctgaaaatgg agctaaatta tttgatgata tagaagatac
    14161 tcttaaatta ctatctaaaa aatataaatt atttatagta agtaattgtc aagctggata
    14221 tatagaatct tttttaaaag cacataaact agaacagtat tttatagatt ttgaatgtcc
    14281 tggtaatact ggtttgcata aaggtgaaaa taataaactt ataattgaaa gaaataaatt
    14341 aataaatcca atctatgttg gtgatacgca aggtgatgca aatagtgcta agtttgtagg
    14401 tatacctttt atatatgcca aatatgggtt tgggaatgta gatgtatatg attattttat
    14461 agactcattt aaagatttgt tagaacatga tatattaaaa taagatgagt ttaaaaatta
    14521 tattaaaata ataaaaaagt tatgggggga tttaaaaatg gaattacaag atactatttt
    14581 taaaagacaa agtgtaagaa agtttaaaaa tcaagatgta ttagatgaag atattttaaa
    14641 aatgataaaa gcagcaggtg cagcaccatc aggcaagaat attcaaaact ggcattttgt
    14701 tgttataaaa cgccgtgatt taatggaaaa gatagcagat gtgataacta aaaagcaaca
    14761 agaaatactt gtagaaatgg ataaagtatc agtggataag gctaatagat ttagaaaatt
    14821 tgttaaaaac tttacactat tttacttaaa agctccagtt ttagtattag tttttacaaa
    14881 ggtgtataac ccatcaggat attatgaatt agagcttata gatgcaccta aggagactat
    14941 agataaatta tttataagaa atccaggtat gcagagtcta ggtgctgcaa ttgaaaactt
    15001 tacactatca gcaattgaac ttggatatgg ttcttgttgg ttgacaagtc aaaactatgc
    15061 agcagatgag atagaagctg ttttagaagc agaaactggt tttgaaaagg gagagtattt
    15121 tttaggtgca atgttagctc ttggagttcc agaggacaat ttgaagagtc catctaaaaa
    15181 accagtagaa gaaatatgta catttataaa ataaaagtca agggcatttt aaaatcgaag
    15241 taaattaaaa acaacataaa ttaaaaataa atatttaaaa aattaaaaag taggttttat
    15301 tgatattaat tttatatttc aaatcattga ttta
//
LOCUS       DAEMAE010000085        14858 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEMAE010000085 DAEMAE010000000
VERSION     DAEMAE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.087"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(84..1391)
                     /gene="grdB"
                     /locus_tag="KPH93_002025"
     CDS             complement(84..1391)
                     /gene="grdB"
                     /locus_tag="KPH93_002025"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(342..344),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG1688845.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIALQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGSI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     IGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(1477..2763)
                     /locus_tag="KPH93_002026"
     CDS             complement(1477..2763)
                     /locus_tag="KPH93_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG1688846.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIALEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCKKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(3042..4364)
                     /locus_tag="KPH93_002027"
     CDS             complement(3042..4364)
                     /locus_tag="KPH93_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG1688847.1"
                     /translation="MKILVVDDELEYGVVMKKILQKKGHLVDIALSGEEAINIIKKDK
                     NYDLILSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQFEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(4364..6358)
                     /locus_tag="KPH93_002028"
     CDS             complement(4364..6358)
                     /locus_tag="KPH93_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1688848.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLINMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIYQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(6445..7692)
                     /locus_tag="KPH93_002029"
     CDS             complement(6445..7692)
                     /locus_tag="KPH93_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1688849.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALLSIV
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMGIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7920..8465
                     /locus_tag="KPH93_002030"
     CDS             7920..8465
                     /locus_tag="KPH93_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688850.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHNNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNIESKVIFAVIL"
     gene            8479..9165
                     /locus_tag="KPH93_002031"
     CDS             8479..9165
                     /locus_tag="KPH93_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG1688851.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     ITGAIDILPYLKKKYTIAIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(9289..9909)
                     /locus_tag="KPH93_002032"
     CDS             complement(9289..9909)
                     /locus_tag="KPH93_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688852.1"
                     /translation="MNYDSNKNKYLLEDINLEDIFVDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYDSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9899..10921)
                     /locus_tag="KPH93_002033"
     CDS             complement(9899..10921)
                     /locus_tag="KPH93_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG1688853.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSAISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYDEL
                     DCWKGMHICGDTRELLDNMLDCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(10922..11560)
                     /locus_tag="KPH93_002034"
     CDS             complement(10922..11560)
                     /locus_tag="KPH93_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG1688854.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     KLNMIKIDKKPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(11678..12829)
                     /locus_tag="KPH93_002035"
     CDS             complement(11678..12829)
                     /locus_tag="KPH93_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1688855.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYNGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIPYEIVEFDETNF
                     EKDDDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGTIAYKKVFIKENNNIIGYL
                     IIRTIQKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDSELLKYMEFKQVENKYIIKYKEEELV
                     IESLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            13251..14063
                     /locus_tag="KPH93_002036"
     CDS             13251..14063
                     /locus_tag="KPH93_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG1688856.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(14151..14342)
                     /locus_tag="KPH93_002037"
     CDS             complement(14151..14342)
                     /locus_tag="KPH93_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688857.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            14593..14841
                     /locus_tag="KPH93_002038"
     CDS             14593..14841
                     /locus_tag="KPH93_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688858.1"
                     /translation="MCNNLQLNKSYFCSLFKKESGYTFTSFSNKIRVEKRKKYLLKKD
                     LSILDVAVLVGFNSQNYYSMVFKKFNNLTPIEYKNIYN"
BASE COUNT         4873 a         2506 c         1563 g         5916 t
ORIGIN      
        1 taataaaaaa agatatcgta aaataatttt aaaaaattat tttttgatat ctttttttat
       61 ttcagtgttg tgttgtattt ttattattca aatactgttt gttcttctat atcttctgtt
      121 agtgcattta aagcttttaa aactatattc tttctaattt ttttactttc ctcttctcct
      181 aacataggat ttccaagtgg atatggtatc cctatggctg gaacaattct attagctcct
      241 attgtaagtg atattggtac tactgtacat atatggacaa ctgctatacc agctctctca
      301 atctctttta ccatcgttgc tccacaacga gtacaagtac ctcatgtaga agttaaaatt
      361 acagcatcta catcatcttc taacaatttc ttagaaaact cttctccaaa tctcttagaa
      421 ttaccaacac tcgttcctgt tcctgtagta gttataaaat aatctactaa ttctccaatt
      481 acaccttctt tttcaagctc tctaactaca tctagtggta caactaaatt tggattttca
      541 aggacatatg ctctatcata ccccccatgt atagttgtaa attctttatt tgacatagaa
      601 tccatatctg ttaaatcgta aattccatat ttagtagcac ttgaagattc aattttgtct
      661 ggattatcaa ttggaactat ccctccagaa gttactaggg caagttttat cttagataaa
      721 tctttaacag gtttcatagg tgtaactcta tcaaattcag gcattggata ctcagtttca
      781 aagctttcac ctttcatctt cttaaccaac atatcaattg ctctttcaga ccctctaagt
      841 tcatggaaga aattttttct tattcctctc attatatatc cttcttcttc aggaggtcct
      901 atttcctcac ctctaaccaa tttaagacct agtttcgtca ttattggaac tacctttcta
      961 agacttgctg ctgaatttcc tgtagacact atatgcaaat ctaatttaaa catatctaca
     1021 cctggatttt ctttatacat tcctgtaact acaggaatat ttagctcttc ttcaactgcc
     1081 ttacaaatac tcccacaagc tactccatat ctacctgcat taaatgctgg gcctgcaata
     1141 aataaatccg ggctttcttc ttttatcatt tttaaaagtt cttgagttgc actctcaata
     1201 ttttcaccaa agtacgtatc tccacaaatt acagttgaga ttatttctgc atcatcatta
     1261 aattgactct gaagtgctat tccaggtcct actacaccag cccttttttc tggcggaata
     1321 tctgcttttt cctctccacc tataccagca aagaaattgt ttatataatg aaccactctg
     1381 attttactca tctcttaatc ctccaaatca ctttatagtt attacatcaa ttagaccaat
     1441 gattacatca acagaacttt atattcaaat acttatttaa taatatcttg ctgtcaaatg
     1501 attatatcca tttgctatag ttgatgctat tataatttgt agctctgcat ccatgcttcc
     1561 gtcttcattt aagcatcctt tatacccacc tatcatcatt tcaacataat ctaaagttcc
     1621 tataattttt tccattgctg ggaaatgaac cactaaattt ccttgaccac aagaaacaac
     1681 tgcatctgct tccttagtag catctgctat agattgactc ttgccatctt ttccaggaaa
     1741 ttcatctgtt atcaaaacta catttgctcc aacttcacta cattttttac aattcatcat
     1801 taggtcagta tctggattcc cataaccttc ttctgtcact aggactccat caacgcctaa
     1861 aaattctatt aatttagcta ccatatcaga gcttctctct ttatcgacta agaatacgtt
     1921 ctcattagtc aaaataactc ctataaagtt taaatctttt ccatgtcttt tatataagtc
     1981 atgtataact ggattattta aatgatggaa tgttgtaacc ttatcacatg gtgctacaca
     2041 gtttccagat attatagctc catccattat ttcagttgga tacataaatg taggcactat
     2101 ctgtttagca tccactccat agtaataagt atcatgtaaa agtccttgtg attgtaacat
     2161 gtgaacatat gctactgttg gcaggtctgg atattctttt acttgtttta ataatggctt
     2221 tgtctcatat acaatgactt catctggctc aacttctctt cctgcctcac ctacatatgc
     2281 acctattttt aatcctgcaa ttctagctgc ttcttcataa tcatgagtat ctataccatc
     2341 ctttgctttt ataacaatac atatattatt tgtttttgaa aaaggtgtat atttagcagt
     2401 aggaccactc atatcaatta caccttcttg aaatccaact atatttccac aagttacaac
     2461 acaagctcct aaaagtacat gggttcttcc actgcctaca gtcttcactt tatttataat
     2521 tccaggaaat attttgcttt catctccaat tttaactcta ggctctatta catctttaac
     2581 tggagctatt cttattgatt cgccaggtct tgcaagttca acatttacac ttacaatcct
     2641 ttcatcttct aatgctatct tttctatttc ttcactacta atatatagta ctccatcttt
     2701 tacttctgtc ttttctccaa attcaacatt ttttataaat atattcccta gctctagctt
     2761 catatgaaaa tcccccttaa tatatttaat cgcaattaaa agtatttatt acgcttatta
     2821 atatataaag cattttctat gccaattatt taaagtatat attctatgta ttaatgtcat
     2881 agaaaatttc atttttaatt tacaccagta ttgaatttac caataattat tgtaaatatt
     2941 aatttttaaa attataataa aaaaagataa acagaatttt tctgtttatc tttttataca
     3001 gtttgagaaa tttttttctc acttaatgag aagttttttt ctcacttaat attatactct
     3061 gatattttat tggttaactg tcttctacta atctccaatt ttttagctgt atttgaaata
     3121 ttatttccac acaaagataa aactttttca atatactcac attcaaactc ttttcttatt
     3181 tctctcaaag gtctaatact tattttatca tctatataca cattatttga tattaaattt
     3241 aaattgtctt ttgatagatt gccttcttca cttaaaacta caagtctatt tattatattt
     3301 tttaattctc ttatatttcc tggataattg taatttaata aaaaatcctt tacttcctta
     3361 tcaatcgagt gtatcttttt atcatgttca aattgatact tatttaagaa aaattctata
     3421 agagtagtta agtcttctct tctctttctt agtggtggta tagttattgt tattgtactt
     3481 attctataaa aaaaatcttc tcttatattt ccatttgata tttccacttt tggttcttta
     3541 ttcatagcac aaattaacct aaagtcaact ttaatagatt tattacttcc tatcctctct
     3601 atagatttgt cctcaagtgt cctcaaaagt tttacctgaa catttaaagg tatatctcct
     3661 atttcatcta aaaatagagt tcctttattc gaaagttcaa atctaccttt tctactatct
     3721 actgctcctg taaaagaacc tttttcatga ccaaacaact cagactccaa tagattttct
     3781 gaaaaagagc aacaatttac aggtacaaat atctcattct ttcttggact tatgctatgt
     3841 atatatctag ctaatatatc ttttcctaca ccagattcgc ccaagattaa tatattaaca
     3901 tctttacaag cagctttttt tgctattttt atgatattat taaaatctct atttttagat
     3961 tccaaagtaa attccaaatt atttttttgt acagaaacag atattttact tgcctttacc
     4021 ttttcaactt cttctaataa attttcaatg ggattacttt ttataaaata agataaagct
     4081 cctcttttca tagcatcaac tgcattttca atacttccgt atcctgtaac taatataacc
     4141 tctatatctc tattaatgga tttaattcta tctaacagct gtactccatc catatttttc
     4201 atcataacat cagataaaat caaatcataa tttttatctt ttttaattat atttatagct
     4261 tcttcaccac ttagtgctat atctacaaga tggcccttct tttgtagaat ttttttcatt
     4321 accactccat attctagctc atcatcaact actagtattt tcattatata gtcacctctt
     4381 tatttaatgg aatttttata tgaaaacaag ttccaactcc caatttactt tcaacactta
     4441 tatctccatt attcttttta atctcattat aagtaatata taaacctaaa ccagttccct
     4501 caccaattgg ttttgttgta tagaagggat taaatatatc ctttagggca tcttctttta
     4561 ttccttcacc attatcttta aaatctataa ataaacaatg atttttttca taacaatata
     4621 taataatttt tccatcttga tatattgcat ctattgaatt agaaattaaa tttataaata
     4681 cgtgttttaa agattctcca tttatatagc atactaaatt atgttcacag tcaattttta
     4741 tttcaacatt ctttaactgt aacattttat attgaagttt cactatattt tcaataaaat
     4801 ttcttatatt tatatgttct aaattatcat ctgatattct tgcgaaattt aacaagttgg
     4861 ttattatatt acttgcccta tctacattac tttctattga tttaacacaa tcatttactt
     4921 cttccattgt aacgttatct tttaagagat agcaattatt tcttattata cctaaaggat
     4981 ttcttatctc atgtgctacc ccagaagcta attgtcctat agatatcatt ttattttctc
     5041 taagtaattt cgcctctact attttatcat tagttatatc tttaataaga actactatat
     5101 actcagtatt atttttttta tactctacag gaaaagtatt tatctttaaa attttattgt
     5161 gtatttctat ttcactagtt ttattagtat tttcagagaa agtattttca ataactcctt
     5221 ttatcatgtt aatcaaatct gctatcttat aactcatttc acccataaaa tcatatagta
     5281 ctttgttcga ttctactata ttgttatttt catctacaag cattattata tctgtaatac
     5341 tattaaaagt tgcctctaaa gtttttttat tttctattac ctgttctgtt tttcttttta
     5401 tttctatctt taacaaatat gtatagctat aaaaaacata aattgatatt aaaattatac
     5461 ctataaaaag ataaatacta ggcacaattc ctcgctcata caatatatca tcaacttcat
     5521 tatatgtaga ataccatttt ttctttaaat ctttatacac tccatttttc tgaagtttaa
     5581 aaataccttt atttaaaata ctaaccagct cttcatattg tttaggtaca gcaagaactg
     5641 cttttttagt atatatagga ttacttaaaa cgctgtattt atttgaaagt ccataattat
     5701 ttatataata tcgcaatact ggttcatcac caacaactgc gtctactttt ccagacatta
     5761 aatggttgac tccagtcttt atatctggag tcaataaaat gtttatatct tgaatttttt
     5821 gctttaaaaa atttatagaa taatcacctt ctggaattgc tactgttttc ccctttaaat
     5881 ccatgtaact atttatatct tgggactttt tatctttcaa tatatttgca ctcaaagtgt
     5941 atattgggtc agtaaatata aatttatttg ccctttcttt tgatggaatc aagtcaaaaa
     6001 actttatact atcatctatt gaatttacga aagattcttt ccaccaatta tttggtttaa
     6061 aataaaaatc tctaccaatt tgaattgaca gagaatttat taaatcaaca ataattccct
     6121 tatactgtcc atcttcctta cctttatatc tcaaaggagg tgaactttgg tcagagctat
     6181 aaatcaaatt cttgtgtttt tctaaccact ttttttcttc ttctgtaaat ggaagtgact
     6241 tcttaatata ttcaaataca tttagattgt actccatatt tacatacata tttgtaagtg
     6301 aaaatactac taaaaatgaa taaataatcc ctataataac aatctttttt ttattcactt
     6361 taaaactcca atcttctttt ttactataat aaattcaata tttaataaca aaatcctact
     6421 tttttcatta aatatttata tttattataa aactaattga atagatgttg aaattatagt
     6481 cattactatt cctatcaatg cttcatatgg aattagctta aatctttctc ctatacccat
     6541 gtttatacta cctgcactag tatgaaatag tgagccatgt ggtaattgct cgaatacact
     6601 agacccagca tttactattg atgcacctga gattggtgat agtccaccat ttattatagc
     6661 atcatgaaaa gtagatgatg ctatagtagc accagctgtt gatgatgctg ttgctaatga
     6721 cataagtata cccgaaactg gtgccaacaa aaattgtggc atatttaaaa attgtagagc
     6781 acctattgta ctctgttgta attcagacat ctgtataatt cctgcgatag tccctgttcc
     6841 aagtaataat atgcatacac cttgcatctt agataatcca aaagctaaat attctcttga
     6901 attgttcaag tttcctgtga caattagagt tacgattcct cctattggaa gggctatcat
     6961 tgggtctata actataggag atacatttcc taaaaacaat agaaatatag ctataattgg
     7021 accacataat gatttaaata aacttggtaa atcttctctt tgctctaaga tttcatatga
     7081 ttgtacttta tttcctttat ttattaatat acttgctaaa ataactgtaa taacaactcc
     7141 aataattgct ggaattatat ttgccatcat aacactagag agattgacag aaaaattatc
     7201 tgctactgct atagtatttg gatttggtga tattatatta cctgctttac cccctcccaa
     7261 catggctaat agtattgaca atttagaata atttaatttt ctacctatat ataatcctat
     7321 aggtgctacc gttattatag atacatccaa atttactcct actccagcaa gtaccatagt
     7381 tgaaatgaca atcgaaagta aagccctctt ttcaccaaat atttttacaa tctgttctga
     7441 tattttatct actgctcctg tttttattaa acttccagct aatacacctg atgccaaagc
     7501 tctaagaatt gatggtgata tattcatggc tccatttgcc ataaaatcta cagtttgtgt
     7561 tatatttgct cctccaacta atcctccaat aaatgctcct aacattaaac tatatacagc
     7621 ttgaaatttt tttataatta aaataattgc tattaataaa ccaaatatag ctccaaaagt
     7681 cgttatttgc ataatatccc ctcctatcat aaattaacta tcttttactt aattttattt
     7741 aaatatattt taagtattct gtatccactt tgtcaattat attttaattt atttaattat
     7801 attgaacata gttttttcaa aacctcattc tatatgttat aataatttca tactatataa
     7861 tcattttact tgattatttt aagttaatat tttttaaatt aaaaagagag gatatttata
     7921 tgaatgattt aaatttaggg gaaaaaatag ctgaagttcg aaaaaaacaa aacttaagta
     7981 ttcgagattt agcaaaacta gctgatgtta caccatcatt attgagccaa atagaacgag
     8041 gtcttgctaa tccatctgta aattcattaa agtctatagc ctcttctcta aacgttcctt
     8101 tatttacatt ttttgtatct gaagtagata aaaaaaactt gatagtaaga cataataata
     8161 gaaaaaaagt tattttacct ggaagcaaag aagttatata tgagatttta attccagact
     8221 ctagtggtaa tttagaattt gcaattatgg atttagctcc aaacacttct tcttgtgttg
     8281 ataggattac tcataatgga gatgaaattg catatgtttt ggagggagaa gttaaacttt
     8341 ttatggatga tgatgaattt actttgtcca aaggagatag tgtaaaagtt cctttaggaa
     8401 caaagcataa atggcaaaat gactcaaata ttgaaagcaa agtaattttt gcagtcatct
     8461 tgtaaaggag taaaaaccat gaagacatat aaatttatat tttttgatgc agatgatact
     8521 ttatttgatt ttaagaaaag tgaatcacat gcttttaaaa aacttctatc tgagtttgat
     8581 ttagaattta attttgagaa ttatatagag tcatatagaa atataagtga taagctttgg
     8641 ctcgaccttg aaaaaaacat tataactttg aatgaactta aattacttag atttgaactt
     8701 tttgcaaata aaatatcttt ggatgttgat tctgaaactc ttagcaaaat gtacttaaat
     8761 tttttaggtg aatgtacctt tttaattaca ggtgcaatcg atatattgcc atatcttaaa
     8821 aaaaagtata ctattgctat aataacaaat ggaatttctg cggttcaaaa gaaaagactt
     8881 gaaaattcaa aaataaaagg atatattgat ggaatggttg tatctgaaga gttgaaaata
     8941 tcaaaaccaa atcctgaaat atttaaatac gctctcaaaa agtttaattg tcatgataaa
     9001 tcttctgcct taatgattgg ggatagctta acctctgata tcttaggtgg aataaattca
     9061 ggaatagata cttgttggtt aaattccaat aacagtataa attatactaa tcatatacca
     9121 acttatgaaa taaatacact tattgaatta aagaaactac tatagtttgt aagtctgttc
     9181 aaattaaata gaatattact ttgcaataat ttttttagtt tatacataaa atgaccaaaa
     9241 aaattattgc aattcataca caaaacaaat cttagtcttg aaaattaact aaaagcattt
     9301 tctaggacac ttactacatc ttgattctaa attttcttca tcccccatta aaataagcat
     9361 tgcaacactt tttatggggg atagttgatg ataatcattg atttctattt ctatactatc
     9421 atatttattt tttacatagt tataaatatc cttttgtctc tctacttcaa aatctttatc
     9481 tcctggatat atttcatgcg atattttgag actatcatac tttcctttta aatacatttt
     9541 tattttctca ttaatataat ctaaacatac tatacctatt ttatccaatg ctagccctgc
     9601 catcatattg caattttcca tatagtcatt catcttaata tcaattttat cccctattgt
     9661 atatagtaca gcaaatgctt ttttacattc tttaggtgtt ttattaatat caacttcact
     9721 tatatatatc ttgatatcta aaatatcatt tatatttctt atttctcttt caactatttc
     9781 actaattgta tctggagctt ttctatttcc ataccccaaa aactttagca caatattctt
     9841 atctacaaaa atatcctcta aatttatatc ctctaaaaga tatttatttt tattactatc
     9901 ataattcatc aatatcaatc ctatctaatt ctttataact aattcttcct gcctttatag
     9961 ctgcttgtaa attttctact ggagtggtat ttaagcagtt acatccaaaa tcacataaaa
    10021 agttatcata tcctctcatc ttctttaaca attttagcgt ctctcttcta attttatctg
    10081 gcttagaacg tccaagaagt tttattgggt ctaaattacc aatcaacact atatctctag
    10141 gaatcattgg agcaatctct tcatagtcta aaatttggtc taaacttact gcatctatat
    10201 tacaatctaa catattgtct aataattctc tggtatctcc acatatatgc atacctttcc
    10261 aacaatcaag ttcatcgtag attttattta aattttttac tatatattca tcaaatctat
    10321 ctttacttag ggttactgat gtcggctctg ctattgaaat atattttgct cctgctttat
    10381 ttgcagctac agcatatctt cttaccaatt cagtcgtaaa ttccaataat tcttctacaa
    10441 attttttatc agttattata catcttaaaa gttgagtagc tccagctaat tgacctgcaa
    10501 gtgtaaatgg accttgtatc gattcatata gtggtttttt aatacttttt gatatagctg
    10561 ataagctctc tatattgagc ggcatacgtc cactcgtata agggtctgga acatctaatt
    10621 ttcttaattt atctctgtca gttatagtat gtgtcaatac acttggaaaa tcataatctg
    10681 gtcttaatgt ctctaaaccc agagtttcac aaaatgtaat cccatcacag aatgagtata
    10741 taaaatctga ttcaaaatac ttgtccatag tctttgctaa ttctaattgc aaatctacac
    10801 tatcatatac ttcatacgcc tttttattta ttaagaataa tccatttgta cccatatcag
    10861 gcagaaaaaa acctctatct tcactattta tatactgaag taaagtcata tctgaaaaca
    10921 tttaaacttc acctcctata agtttgttta ttaatttaac tgcttttgga gcatttgaac
    10981 tgtatccatc tgctcctatt tcttctgcaa actccatact tacaggacct ccaccaatta
    11041 ttacttttgg ttttttatct atcttaatca tattaagttt gtctataacc gatttcatat
    11101 tctccatcgt agttgtcatc attgaagata agcctattat atctacattg ttttttatag
    11161 cttcttctat tatatcttca ctttttctat ttacacctaa gtcaataacc ttatatccag
    11221 tggcttctac cattactttt acaatatttt ttcctatctc atgagtatct ccttctacta
    11281 cagacatcag aattactccc tttgattttt tctttacttt tccagttccc ttcaaaagat
    11341 taataccttt atttaatgca tctgtacata ctataacttc tgaaagatag tattctttat
    11401 tctcaaactt atctaaagct cctatcatac ctttgtttaa accatattca taaatatctt
    11461 ccacatcaat atttttatct atagcaattt ttactaaatt ctctatatta tctacaccca
    11521 tatttaatat acattctgct atatcatcaa gtatgtccat ataatactca tcctttctaa
    11581 ttacttacaa aattgctttt tcaatatcca ataatttctc ttatttaaca aggactaact
    11641 ctaggtatca atcaatttta tttaatatca tctaaaacta ttgataattt aaatttgcag
    11701 tccatggaaa atctacagga aatgcctttg tagcaaattc atatatattt tttagatgtt
    11761 taaattcatt gtactgttct tcattttttt caaaaaacag tttattaagc ttatccaaac
    11821 tttctattac aagttcttct tctttatatt taataatata tttattttca acttgtttaa
    11881 attccatgta ttttaacaat tcagaatcta catattgact aaaataactt cttaaactat
    11941 cacacaactt agtaaaattt attattttca tagttccttg ttgatagtcc aaagattttg
    12001 tcccattaca tttcaactgg tctttaagat ttttcacatg tactttataa tttaaatatt
    12061 ctaatccgaa tttatttgca acatatttca atatattttc aacatttaca gagtttaacc
    12121 ctatttcagc aacttctcct acagtagagt cttctttttg tattgttctt attatcaaat
    12181 atcctattat attattattt tcctttataa ataccttctt gtaagcaatc gttccccagg
    12241 caatagttgc tgcatgcaat agtttttgaa actcatctct cgttcttata aatctagtac
    12301 tattttgatt atatagttca atcattttat ctaaatcatc atctttttca aaattagttt
    12361 catcaaattc aactatttca tatggtattt ttacatcttc aggttttatt gtatatttat
    12421 agaaactctt taccaaacta caatttcttc tactgtatag acttcttgtt cctgatataa
    12481 ttacaatatc tacaccatta taaaacattt tttcttctac cttatccaat atcttagatg
    12541 aatagcctct cttttcataa tctggatgag tacatactcc acctatagct gcaaccttca
    12601 atctgtttcc ttgaatagat acatcttgaa ttagatagtt tacatcagaa accactttat
    12661 catcttctga tattataatc atattttcta tattattttt acttagtaaa agaggaaatt
    12721 cttccatcat agttggttta tgatttcttg atatcctaaa tacatagttt attaattcta
    12781 ttactttatc aaattcttct aattttgcac ttcttggtct taattccatt catattcctc
    12841 caaatattat ttaagatact taaaatttta ttgtctataa taaaacattt attactacag
    12901 ttctaattat atcttttttc tttacaattt ttaattgttt ttttcagcaa atttttataa
    12961 aatataaacc taatttctag ttaaacttat aaaataaccc attatgtata atatattaaa
    13021 atattttcta tatatttata tgactaagtt ctataactaa attgaaataa tcactttttc
    13081 aagaatgtta taattttttt attgtattct ttttgctata gtctataaat aaattaatag
    13141 aaaatcttct aagttacttt taaataatga ctttatgatt caattgtact aaaatagtat
    13201 atgtaggtta caaataatct aataaatatt aaagtttgga ggtaatagat atggcagatt
    13261 gtaattcatg tccatcaaag ggaaattgta atagtcaatc aaattgttct attgaaaata
    13321 accctaacaa taaatttgga aaaataattg gtgttatgag tgggaagggt ggagttggaa
    13381 aatcaactgt aactgcttta cttgctaata agctaaacaa aatgggatat aaggttggta
    13441 tattagattc agatataact ggaccaagta ttcctagact tatgggtgta aaaaatgtaa
    13501 aagcttattc tgatggctct tatatttacc ctgtagaaaa ctcaaataac ataaaagtaa
    13561 tgtctataaa ccttatgata gatgatgaga atgaacctgt tgtttggaga ggacctttac
    13621 ttggaggagt tgtaaagcaa ttttatactg atgtactttg ggaagaatta gactatttat
    13681 taatagatat gcctcctggt actggagatg ttgccttaac ggttatgcaa tctattccta
    13741 taagtggaat agttatggtt tcagtacctc aagatttggt atctatgatt gtatcaaaag
    13801 ctgttaatat ggctaaaaaa atgaacataa atgtccttgg agttattgaa aatatgagtt
    13861 atatccaatg ccctgattgt tctaaaaaaa tcaagttatt tgaaggagaa tctactgaaa
    13921 agtttttaga tgatttagac ttagagcttt taggagaact tcctatgact aaagaaatta
    13981 tagatattac tcataatgga gttacagaaa ttagtgatga tttagattca atattgacta
    14041 atgttgttga aaaaattaaa taaataaaaa ttttataatt ttatatttat caaaaaagag
    14101 gtttatattt attttataat catacaaata aatataaacc tcttttatgt ttatctatgt
    14161 tttaaataca caaaatattc ttctacttca tctccatttt ttacttttac taagtattca
    14221 ttataatgag cattatatgg ctgtgtcatt ttgcattcta tcacattttc tatgtcttct
    14281 aattctaaaa gttctacatc ctttaaaaat tttctttcta tgagttccat taatctttcc
    14341 ataatatatc ctcacttccc ctataattaa gataaactat atatcttctt aatacagagt
    14401 ttatctgttt attatattat atccaatata ttacagtttc taacataatt cataaattat
    14461 ttaacaaata gagattgatt ttttaataaa tatgccttga taaaatgaaa catagaccaa
    14521 ctcttagttt tatgtaaaaa aactatagat tatattcaca aaaattactc ataagatata
    14581 acagtaagta atatatgtaa taacttacaa cttaataaaa gttatttttg ttctttgttt
    14641 aagaaagaaa gtggatatac ttttactagt ttttcaaata aaattagggt tgaaaaaagg
    14701 aaaaagtatc tattaaaaaa agacttgtct attttagacg ttgctgtact agttggattc
    14761 aatagtcaaa attactatag tatggttttt aaaaagttta ataaccttac accaattgaa
    14821 tataaaaata tttataacta aaaaatatca ttctaaaa
//
LOCUS       DAEMAE010000086        14234 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEMAE010000086 DAEMAE010000000
VERSION     DAEMAE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.088"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..901
                     /locus_tag="KPH93_002039"
     CDS             134..901
                     /locus_tag="KPH93_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG1688859.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1185..1382
                     /locus_tag="KPH93_002040"
     CDS             1185..1382
                     /locus_tag="KPH93_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1688860.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1411..2487
                     /gene="vorB"
                     /locus_tag="KPH93_002041"
     CDS             1411..2487
                     /gene="vorB"
                     /locus_tag="KPH93_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG1688861.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2490..3209
                     /locus_tag="KPH93_002042"
     CDS             2490..3209
                     /locus_tag="KPH93_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG1688862.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTVEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3222..3761
                     /locus_tag="KPH93_002043"
     CDS             3222..3761
                     /locus_tag="KPH93_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG1688863.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3918..4976
                     /gene="buk"
                     /locus_tag="KPH93_002044"
     CDS             3918..4976
                     /gene="buk"
                     /locus_tag="KPH93_002044"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG1688864.1"
                     /translation="MKVLIINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIKIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEVVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4994..5887
                     /locus_tag="KPH93_002045"
     CDS             4994..5887
                     /locus_tag="KPH93_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG1688865.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIENISREIGLKAHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5917..7125
                     /locus_tag="KPH93_002046"
     CDS             5917..7125
                     /locus_tag="KPH93_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1688866.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLELLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7149..8408
                     /locus_tag="KPH93_002047"
     CDS             7149..8408
                     /locus_tag="KPH93_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1688867.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGIVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEKEEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            9050..10513
                     /locus_tag="KPH93_002048"
     CDS             9050..10513
                     /locus_tag="KPH93_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG1688868.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMAL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLITCAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRHLKEDKHELN"
     gene            10569..11705
                     /locus_tag="KPH93_002049"
     CDS             10569..11705
                     /locus_tag="KPH93_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1688869.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVAPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLIDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLSGIFTKIMEEFGIYEDDQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            11941..13056
                     /locus_tag="KPH93_002050"
     CDS             11941..13056
                     /locus_tag="KPH93_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG1688870.1"
                     /translation="METLEVRNIELSGTNYEIGYKLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMLELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKGVNRISGLENKREHFLHSTNHIHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKDDIKSITQRISINIEKAPSDFFDFIE"
     gene            13207..14076
                     /locus_tag="KPH93_002051"
     CDS             13207..14076
                     /locus_tag="KPH93_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1688871.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKEIGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEEIKVIGIKDKVILENNSLPD
                     LWEKFRKIHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGKLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPN
                     NVNSQIDIYIPIK"
BASE COUNT         5518 a         1524 c         2749 g         4443 t
ORIGIN      
        1 tttacaaatt aagtataata tttttgtttt ataaaatata attaagataa atagataatt
       61 ttgtaatttt aatatataat atttagtaga gtgtgggagt agcacttaat atttgcagga
      121 ggagtttttg tatatgggag aaataataaa tgcattagat agagccctag acataatttt
      181 gctgttatat catgaaaaga gagaaatggg aataactgaa ataagcaagg ctatgggagt
      241 atataaaagt actgtacata gaactttggt tactcttgaa aacaaaggtt ttgttataca
      301 aaatgcagag aatggtaagt attggctagg aataaattta tatgcaatag gtatggttgt
      361 gggagaaaaa atgtcattga ctgaaatagt aaaaccatat acgaaaaaat taaatcaaga
      421 atttaacgaa gtagttaatg tatctatatt agaagaaaga gctcaagatt ctccaagaag
      481 tatcataata cataaagagt atggttcaaa tcaattactt tcagtaaatc catctgttgg
      541 ttcaagtagt gaatgttatt gttcagcagt tggtaaatgt cttatggcat ttaatgattc
      601 tatagatttt gaaaaatata gaaaaacacc aatacacaag tatacagaac atacaattga
      661 taattgggat gatatgatgc tttttttagc aaaaataaaa gaacaaggat atgctataga
      721 tgatgaagaa ctagaacatg gactaacttg tattggagca cccatcttag ataaaaataa
      781 taaagctatt gcagccataa gtttgtcagg tccaactata agaatgagag aaggggactt
      841 tgagtataag attaaaagag ttatagaaac tgcaaaaagt atatctgaac tttttagatg
      901 atttttaatc tcgataataa aaagaggcta catttaaact gttatttgta aatatcagtt
      961 taaatgtagt ctctttttat ttaatctaaa attaaatatt cttaaaattt aaaatgttat
     1021 tgaaatatat aagcgaatgt gttatctttt aattaagcaa taaaaaatag aacaaggttc
     1081 tatactatag aacaaaattg atgcatatat agtatttata caaaatataa atattatata
     1141 tttaaagtta agtaaaaata ttacataaag gggagatttg agaaatggaa aaagtcaagt
     1201 tgagtgttga aagatgtaag ggatgctatc tttgtattga agcttgtaaa aaagatgcaa
     1261 taagtctttc tgactacaca aataaaaaag gctttgtaac tattaaagtc gatgaagaaa
     1321 aatgtattca atgtggtgct tgttatacaa tgtgccctga tttagtattt gagatattat
     1381 aaattatttt tattagaggg gagaaaaggt atgcaaagtt tattaattaa aggaaatgaa
     1441 gctttagcag aaggtgctat acgtggtggc tgcagatttt atgcaggtta tccaataaca
     1501 ccacaatcag aaatattaga aatgatggca tggagacaaa aagaagtcgg aggagtattt
     1561 atccaaggtg aatcagaaat agccagtgta aatatggtta tgggagcaag tgcacttgga
     1621 gcaagagcta tgacttcttc atctggtcca ggattttcat taaaacaaga aggtattgca
     1681 tattgggtat cagctggttt gccagcagtt gtagcatgtg tgcaaagata tggtataggt
     1741 gatggattta taggagctgg tcaagataat tattggcaag cagtaaaagg cggtggaaat
     1801 ggagattatc atttaatcgt gttggcacct aatagtgttc aagaaaatat ggatatggca
     1861 tatgaatctt ttgaattagc tgaaaaatat atgcatccag tacttatact ttctgatgct
     1921 acaataggtc aaatggtaga accttgtata gcaccaccaa tgaaagaata cgatatggat
     1981 aaagctccat gggctgcaaa aggaacacct gtaggagaaa aaaataaagt aataactgat
     2041 ataacatatt ttgaagacca agatacatgg caaaaagggt atatagaaaa aatgagaaaa
     2101 gttcaagaag atgaacaaag atgggaagag atagaaatag aagatgctga agtggtattt
     2161 gtggcatacg gtatttcatc aagaatagca gaaggtgcag ttagagatgc tagagcaaaa
     2221 ggaatgaaat taggtctaat aagaccaaaa acattgtggc cttacccaag aaaagcattt
     2281 aagaaattaa gtgattcatt gaagggactt gtaacagttg aaatgaatat gatggcacag
     2341 atgaaggaag atgtaattat agcatgtgat aatagattcc cagtatattc agtagcaaca
     2401 gctcaatata tggcagaagt aaatcaatta atagattttg ctcaacagat tatagatgga
     2461 aaagctaagc aagaggaggt attttaatta tggctaaaaa agcaccagca ataatgatgt
     2521 gtgaaaatat gttctgtgga ggctgtggac actctatagt cgatagaatt attggagaag
     2581 tattaacaga aatggatata tgtcaagata caattatatg ttcagatata ggttgtaatc
     2641 atatgtttca atttagtatg aatgtagatg ttgtcgttcc acctcatggt aaaatgggag
     2701 cagcaatggc agctatgaaa agagttagac cagataaata tatatttaca atagctggag
     2761 atggtggagc ttatgcaata gggcttggag aaactatgtc agctgctact agaaatgata
     2821 atgtaatatt tcttgttatg aacaataata tgtttgcaat gacaggaggg caaatggctc
     2881 caactactgt agaaggtcaa tatacagctt ctactccagc gggtagaaat tacgcagatc
     2941 atggttcaac atttgatgtt gtaaaagtta tgggaaattt agatatagca tatctagcaa
     3001 gaggaacaat aactaaccct aaagaaataa atacaacaaa aagatacatc aaaaaagctc
     3061 tagaaaaaca aaaagaaaag aaaggttttt gcttaataga agtgcttgta ccatgtccaa
     3121 caaactgggg attaaagcca gtagatgcta tgaagagaat tgaaaatgaa cttgttccaa
     3181 agtatccact tggagaattt attgattagg gaggaaataa tatgttgaat gaaattatat
     3241 gtgctggttt tggaggacag ggagttctta cagcaggaaa aattataatg tatgttgcat
     3301 ataaggcaga aaagaaaatt acatggttcc catcttatgg aaatgagatg aggggaggaa
     3361 gtgcaaattg caatgttgtt gtaagtgatg aaaaaattgc aaatccttgt gtaaaaaatc
     3421 cagatatact attgggaatg aacagcgtat cagtagataa gtatgaaagt ttaatgaaaa
     3481 aaaatggtta tatgtttgtc aatgaatcac tagtaagtaa ggatagaaaa tacagagaag
     3541 acattaatgt tataaaagta cctgttactg aaatagcaca aaatttaggt aatgaaaaag
     3601 ctgctaatat ttgcatgtta ggtgctatgg taaagagttt agatttattt acaaaagaag
     3661 aatttgaaag tggtatgtgt gaatactttg aaaatcaagg caaaggtaaa ttcaattcta
     3721 aaaatgtaga agcatttgaa gctggatacg actacgaatg aatattttaa atatttaaaa
     3781 taattattga aatacttatt tttatatgtt agtatttaat taataaaata tagaacgtcg
     3841 ttctggacaa taggacgaag tctttgggct gaaatacaag aacgacgttc tatatagtag
     3901 aactaggggg tgttaaattg aaagttttaa taataaatcc tggtggtaca tccacaaaga
     3961 tttctgtttt ccaagatgaa aatgaaattt tgaaaaaaaa tataacccat acaagagaag
     4021 atttaaaaaa tttttctaaa gtatttgatg aatatgatta tagaaaacaa ctgatagtag
     4081 atattttaag tagtgagaat catagtataa attcttttga cgcagtagtt ggtcgtggtg
     4141 gacttatgaa ggctataaaa ggtggtacct atactgtgag tgaagaaatg attgaggata
     4201 tgagaaatga aataaatggg gaacatgcat caaatttagg tgcattatta gctaaaacta
     4261 tagctgatga aataggggta caatcatttg tagtagaccc agtttctgta gatgaatttg
     4321 atgatgtatc gaggattaca ggaatatcag acatagaaaa agcaagttgg ttacatgcat
     4381 taaaccataa agctgtttca agaaaaattg cacaaaaact agggggaaaa tacgaggatt
     4441 ataactttat tgtagctcat ttaggttcag gaatatcgat tgtagcacat aaacaaggca
     4501 aaatgattga tggaagtgga ggtaggtctg atggtccatt ttctccagaa aggagtggtg
     4561 gattacttgc atatcctctt atagaattgt gttactccgg aaaatatact catgatgaaa
     4621 tggtcgcaaa agtaagctcg attgggggaa tgtacgatta tttaggtaca aaggatatga
     4681 taaaaattga aaacagaatc aaggagggtg atgaaaaggc aaaacttata atggatgcat
     4741 ttatttatca agtagcaaaa gaaatttcta tgtatggagc ttcactttca gggaatgtag
     4801 atagagttat tttaacagga gggatatctc actcagaaat ggtagtcaaa ggagtagcag
     4861 aaaaagtttc atatctagct ccaatagagg tagtaccagg agagatggaa atggaagcac
     4921 ttgctcttgg agcacttaga gtactcaacg gagaagaaat agcaaaaact tattaatatt
     4981 taggagaaag agtatgttaa aagattttaa taatttatta gaagaggcta aaaagcttga
     5041 acctgttatt gtaagtgttg ctgttgcaga agacaaagaa gtattaaagg ctgtgaaatc
     5101 agcaacagat ttaaacatta taaagccaat tcttgtagga gacaaggata aaatagaaaa
     5161 tatatcgaga gaaattggat taaaagctca tgagataata ggctgtcaaa atcctgatga
     5221 atcagttaaa aaagctgtag agatagttaa aaataaggaa gctaaagttc ttatgaaggg
     5281 attagtaaat acgagtgtct atatgagagc tatattaaat aaagaaacgg gtcttagaac
     5341 aggaagatta ttgagtttgc ttgcagtata tgaattacct caatatcata aacttcttta
     5401 ttgtactgat agtggaataa atgtatctcc aaacttaaat caaaaaaaag atataatgac
     5461 aaatgcactt cttactatga aaagtatagg aatggaaaat cctaaaactg ctatacttac
     5521 tgcaaatgaa atggtagacc ctaagataca atctacagtg gatgccaact tacttgtaga
     5581 gatggaaaaa tcgggagaaa taccaaagtg tatagtggaa ggtccaatag catttgatgt
     5641 agcatttgat gcacatgcag cagaacataa aggtattaaa agtaatattg ctggtgatgt
     5701 tgacttatta gtatttccga atatagaagc agggaatata ttaggaaagt catggcttag
     5761 atttaataaa gctaagtggg caggtatagt tttaggggca acaaatccga taattttagg
     5821 ttcacgttct gacacagctg aaataaaaat aaattcaata gcattagcgt gcctagcatc
     5881 acaataagaa attattatta aggaggaaaa agtattatga aatatgcaaa aagaatggat
     5941 atggtaaaag cttcagctat aagagattct caaaagaaaa tagctcagaa ggtagcaagt
     6001 ggtggaactg ttatatcttt tgctgcaggg cttcctgacc caaatttatt tccaatagaa
     6061 gagataagtg aaataactca aaaagtactt aatgaaagag gaaaatttgc cctagcatat
     6121 ggacctacta aaggtgaaga tgagttgtta gaactattag taaaaagaat gaaagaagaa
     6181 gaaaatattg atacaaaagc agaaaatatt ataataacta ctgggtcaca acaagggata
     6241 gcattaagtg ctatgatttt attagaaaag ggagatatag tagttacaga aaatccaagt
     6301 tacttaggtg caataaatgc atttagacct tatgaatgtg attttttagg agtagataca
     6361 gatgaagaag gaatggtaac agatgattta gataaattat tatctgaaaa ttctaacata
     6421 aaggtgatat atgtaatacc aacatttcaa aatcctacag gaaaaacatg gtctttacaa
     6481 agaagaaaag attttatgga agtcgtgaat aaatatgatg tcatagtaat agaggataat
     6541 ccttatggag aaataagatt tacaccagaa cctctaccta cacttaaatc tcttgatact
     6601 aaagataaag tattgtacct tgggtcattt tcaaaggtat tatgtccagg atttagagtt
     6661 gcttggatgt gtggtaatgt agatatcata gataaggcag aacttttaaa acaaggtata
     6721 gatttacagt caaatcaatt ttcgcaagta caagtaatag aatttttaaa gaaatacaac
     6781 ttaaatgaac atatagaaaa aataagagca gagtataaga aaagatgtct gctaatgtta
     6841 gatgctatga agaaacattt cccagaggaa gctaaatata cagaaccaga tggaggaatg
     6901 tttatatggg ttgaacttcc tgaaagcata aatgtagatg atttattaga taaagccatt
     6961 gatgctggag ttgcttatgt ttctggagag tccttctttg caaataatgg tccaaaaaat
     7021 actatgagac ttaactatac aacaatgtca gaagaacaaa tcgttaaagg tgttgagata
     7081 cttgcagaag ttataaaaaa agaactcaat tatgcttgtg aataaatata aaaagtgagg
     7141 gtttaaatat gggagaaaaa gaaaacggta taaagtttgg aaagttaatg tttcaacttg
     7201 ttattatagc aatatcttgg gaattagttt atattatacc atttatccaa tatacattat
     7261 atgatccaat cctaaaagca ttagaatgta caaacactca actaggtttt ctacttacaa
     7321 tttatgggtt aggtaatata tttggagctc ctataggagg ctggttagca gatagatttg
     7381 attatagaaa gataatttta ggttctgtat ttcttaatgg aatagtatca tttttgtttg
     7441 catttaatat gaactatcca tttgcagttg ctacttgggt aggttgtgca atcacatcat
     7501 tagttatgaa ctatccttct atggtaaaga tacttagagt tataggaaaa gataatcaag
     7561 gtaaggttta tggatttaat gaagctatgg ttggagtttc tggagtagta atgggagcaa
     7621 tattcttata tatttataca tgctttgcaa ctcctacatt aggtatgaga tgggtaatga
     7681 tatctttagg aatattatca atagcaatgt gtccagtatt atggtttgta attaaagatg
     7741 tagatattaa agaagaaaaa gaagaagcag gtgaaaaaat gtctgctggt gattttataa
     7801 cagtactaaa atcacctaat acttggctag taggaataag tatattctgt gtatattcat
     7861 tcacagttac aatgtcttat tttacaccat atataacatc tgtacttgga ggttctgtag
     7921 cactatctgg tgcattagca ataataagac aacatggtct aaaattgttt ggagcaccat
     7981 ttggaggata ttgtgcagac aaattaaaat ctcctacaaa agtactttta ccaatatatg
     8041 tatttggtat agcagtaata gttttatttc tagtattacc tgcatcaact ccaatgacta
     8101 tattcatagc tttaacattt gtagtaggta tattaggtta catgggaaaa ggtatatatt
     8161 atgcagtaca agatgaagta aaagttccgg ttaaatattc agcaactact ataggtatag
     8221 ctgcagcttt aggattttca ccagatgtat tccaatttgc attaatagga cactggatag
     8281 atacttatgg caataaaggt tacacatata catttatatt ccagatagca attctagtta
     8341 ttggaatact atcttgttta tatatattaa aggttaaaaa gagaagagca agtaaattag
     8401 aagcttaaat attaaatttg gtgattaagt atatataaat acaatactta ttttgatgaa
     8461 tgtaattgta taaataacat aaaaaatatc aatagattaa gttttataat aatgtttaga
     8521 gaataaagta taaaaatcaa aatgttatca agttttctag gttttaacat aaagttaccc
     8581 ctgaaggtgt aaaattgagt gtcaattatt atctattttc ttacctacat aaacctattt
     8641 aatttaaatt ctaagagaat ttcaaaatag atggtagttg tactcaattt tagacctcat
     8701 aataaatacg aaaatttaaa atctaactag atactattta attatatatt aataaatata
     8761 tattgtacta tttatataat aatttcaggc ttttcattat atgtatttca atttacattg
     8821 tttgattatt attcaaacac acgtcgttaa aaattttaaa aatttatgtt tagatatgat
     8881 taatactctt gattttagaa ataaatattt gtagattgta gattaaagat aatataaata
     8941 aatagaaggt agatttttta gatatattaa gataaagatt atataaaata ttgaaatata
     9001 gctgtttaaa ttattgtcta taaatataaa atgaatggtg gtgagttaag tgaccgattt
     9061 agttaacacc attaatggaa ttgtttggtc acctgtatta gttgcaatgt gtttgtgtat
     9121 aggattatat ttttcattga aattaaaatt ttttcaaata agagatgtaa aagaaatgtt
     9181 tactctcttg ctcgaaggag atagttccca tgttgggata tcatcatttc aaggttttgc
     9241 tacatctatg gcgggaagaa tagggactgg aaatatagct ggtgtagctg tagcaatagc
     9301 aatgggaggt ccaggagcat tagtatggat gtgcataatg gctacattag gttcagcaac
     9361 tgcatttgta gaatctactc tagctcaaat atataaagat gagcatgatg ggcagtatag
     9421 aggaggacct ccatattatt ttgaaaaagg tcttgggtgg aaaaagtgtt cagttttatt
     9481 ttcaatagtt gcaataataa gttatacatt ttttatgcct ggtacacaat caaacacttt
     9541 atatctagca atgaatcaag catttggatt ttcaaaaatg acaatagcag tagcatcttc
     9601 tattgcatta gcgataataa tttttggagg aattaaaagg attggtttat ttgctgaaaa
     9661 agtagtacct ataatgggtg gagcatacct tattatggca ttagtaataa ttataacaaa
     9721 tattagttta gttccagaag ctattgtaac aatgtttaaa tctgcatttg gatttggttc
     9781 agtttttgga ggaatgttag gttcagcaat atcttgggga gtaaaaagag gtatatactc
     9841 aaatgaagca ggagaaggtt ctgggactta tggtgcagca gcggcagaag taagtcatcc
     9901 agccaaacaa ggattagttc aagcattttc tgtttatgta gacacattat taatatgtat
     9961 ggctacaggt attatgattg taataacagg aatgtataat gttacaggtg tttgcacaac
    10021 actgccaggt gtagaaccgg ggccacagta tgtacagagt gcaataaata cattgcatcc
    10081 acaattaggt gatattttta tagcaatagt tttattatta tttacattta caactttatt
    10141 agccttctca tatatgatgg agactaatgt atcttactta gtaactagaa ttaaagcaga
    10201 aaaacatgaa aagtacataa ttaatgtctg tagatttatg cttataacat gtgcagcaat
    10261 gtgttgtttt atagaaccac ttacaagttg ggcattaggt gatatatgta taggtgtatt
    10321 aacatatata aatttaattg ccatctttac tctaaaaaaa ccagctataa aagcatatta
    10381 tgattatata agacagaaaa agatgggaat aaaaaggagt gatagaactt ttaaccctat
    10441 agaattagat ataagaaatg ctgagttttg ggaaaataga cacttaaaag aggataagca
    10501 tgagttaaat taaaattata ataaaatata aaaattacta aataaattta gtggggggta
    10561 agattggtat gaaagaaaga ataatacaag tggctgattc aaagaaagaa aaaattctag
    10621 ggctatgtca atctttatat gatgaaccag aaattgcttt gcaagaatat aaatctgcta
    10681 aaaaaatatc agaatttcta agagaagagg gttttgatgt tgaggaaaac ttggcaggta
    10741 tggctacagc ttttaaagca accaagaaaa atggagatgg tcctaaaata gcatttatag
    10801 cagaatatga tgctcttcca ggcaatggcc atgcatgtgg acatcattta attgcatcta
    10861 tgggagttgg agctggaata gctctttcaa gtatactgga tacttacaag ggagaagtaa
    10921 gtataattgg aacacctgct gaagaaactg gagatggaaa gccttacttg atagaacatg
    10981 gtgtatttga tggctatgat gcagcaatga tgatacatcc aaattcgaaa acttgtgtag
    11041 cacctgaaat tatagcaata ggaggtctag atttcatatt tacaggaaaa gcatctcacg
    11101 caggagctaa gccatataac ggaataaatg ctttagatgc agtagtacta ctatataata
    11161 atataaatgc actaagacaa caactaatag atggaacgag aatacatggt attatactag
    11221 aagcaggaac tgctgcaaat gtaatacccg atatgggaaa agtaagatta gagataaggg
    11281 caaaagaaca aaattacttt gatgaagtag tagaaaaagt gaaaaattgt gctagaggtg
    11341 ctgcaatagc cactggatgt gaacttgaat tttatcactt tgaaccaact tgtcaaggat
    11401 taaatgaaaa taaagtttta tcaggtatat ttacaaaaat catggaagag tttggcatat
    11461 atgaagatga tcaagtaatg ttaggttcaa cagatatggg aaatttaagt caaataatgc
    11521 catgtataca tcctttgatg aagttttctg aaaatggaga agaactacat acaaaagaat
    11581 ttttagaggc ttctataaat tcatatgcaa aagatagagt aattgatggt ataaaaatat
    11641 tagctttaac aggatttaat ttatttgaaa atccagagtt attgaagaaa atgaaggaag
    11701 agtagaattt agactatttt taattgtact ctttgaaata agaagagtgt ctcaaaatga
    11761 actttttagt ttatgagaca ctcttttttg tatgaaatta aatatatttt tatcatttta
    11821 acaatgaaaa agaggcttat ctgtttttat ataacctttt ttaacttgta gcacaaaata
    11881 tcatgaatga ttacaattca aatgataaaa tcgattataa gatatatagg aggggtacat
    11941 atggaaactt tagaagtaag aaatattgaa ttgagtggta caaattatga gataggatac
    12001 aaactaggag aattggttgc taatatgcca gagataatag aaggtcagat taataaatct
    12061 aatctggtaa gcaaaaaaga agaaaaagag atgttagagt tgtttgataa atattgtcca
    12121 ggtcttaatg aagaattgca aggttttgct gatgcaattc aagtgaattg taatcaaatt
    12181 ttatattata ctatgactta tcttaaacca ggttgtagtc aagttgcatt agcaccagaa
    12241 ttaactgaaa atgggcatgt actatttgca cgtaattttg atttctcaca caatatggag
    12301 gattttgttt tatgtaaaac taaggtaaat ggaaaatatg cacatatagg tactaccatt
    12361 atgcaatttg gaagaggtga aggtatgaat gaatgtggct taggtgtgag tcagtcatca
    12421 tgtggtattc ctgtaggcaa ttcagatggt ttgagaaaac cagcaattgt aggtttacag
    12481 ttttgggctg ttattagata tttattagaa aattgcaaag atgtagatga agcattagaa
    12541 tatttaaaag atatgccaat tgcatacaat ataaatttat tacttgcaga taagtctgga
    12601 aatattgcac ttgtagagac tttagatgga aaaaaaggag tgaatagaat tagtggatta
    12661 gaaaataaga gggaacattt tttacattct actaaccata tacatattga taaacttcat
    12721 aaattagaac cacaatcaat gaaaaattct attcatagat ataaattaat taaagaatat
    12781 ataaataagt ctaaaaaaat tggagagaaa gagttaatga atttattgtc tttaaagtat
    12841 ccaaatggct tatcttgtaa ttactataat gatttttttg gaactttaaa aagtatagtt
    12901 atggatttaa atataggaaa gtttaatatt ttatggggtg gactagagaa taaatgggag
    12961 agctactatt taaaagacga cattaaatct ataacacaga ggataagcat aaatattgaa
    13021 aaagcaccat ctgacttttt tgatttcatt gaataaaaaa tgtatttaat tattctaaat
    13081 aattaggcta tataaatcaa ctaaaagttt ttatttatca tattgaatta gttataatat
    13141 tttagtatag taaaaatagt gttattttgc atacttaaag agtttaagta gaagagaggt
    13201 gccatattgg attacttaaa gtattttgaa aaagctgtaa tgtatattga gaataattta
    13261 catgaaaata taactgtaaa tgatgtggca aaagaaattg gatattctta ttatcattta
    13321 acacgcttat ttaagtctat gtttggtgag agtgtaggaa gttatataaa aaaaagacga
    13381 ttagtaagta gtacaaaaga acttttatat agtgataaaa aagtgataga tatagctatt
    13441 ggtagtggat ttgagtcttc agaagcattt agcagagcat ttaaatctat atacaaggtt
    13501 agccctatag aatatagaaa aaatagaatt gatgtatttg ttggtaaaaa gaaaaaatta
    13561 gaattagatt ttatgagaca tcttgttgga aatataacga ttaaaccagt tataaaagaa
    13621 atagaagaaa taaaggtaat aggtataaag gataaagtaa ttttagaaaa taattcatta
    13681 cctgacttat gggagaagtt cagaaaaata catcatattg tcccaaatac tttgccatca
    13741 aagagagttt ttggaatctg tgaagcaatc tctgaaatac acctagttag tgaaagtatg
    13801 gagtttaatg agataatagg gcttgaagtt aatagttatg atgttattcc aaattcattt
    13861 gtttcaaaaa ctattaagga aggaaagtat gctatattta ctcatactgg taaattagat
    13921 aatttagata aaacttatga atatatatgg ggtacttggt ttttaagctc aaaagaagaa
    13981 cttgatataa gagatgattt tgaagtatat gatgaacgat ttttaggtcc taataatgtg
    14041 aactcacaaa ttgatattta tatacctata aaataatcta taagaaaact aaaataaaac
    14101 taaaaaataa tttgattaag aaatagatgt tcgtaaaaga gcatctattt tttattttta
    14161 tcagatttag ataattttaa taataaaaga aataaaaata agttcttaaa tttctaagaa
    14221 gaatttagaa tata
//
LOCUS       DAEMAE010000087        14192 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEMAE010000087 DAEMAE010000000
VERSION     DAEMAE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.089"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      complement(125..208)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(441..1283)
                     /locus_tag="KPH93_002052"
     CDS             complement(441..1283)
                     /locus_tag="KPH93_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1688872.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNTGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNKGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANPVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1535..3319)
                     /gene="pepF"
                     /locus_tag="KPH93_002053"
     CDS             complement(1535..3319)
                     /gene="pepF"
                     /locus_tag="KPH93_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG1688873.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSIAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3385..4050)
                     /locus_tag="KPH93_002054"
     CDS             complement(3385..4050)
                     /locus_tag="KPH93_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG1688874.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVKNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4201..4362)
                     /locus_tag="KPH93_002055"
     CDS             complement(4201..4362)
                     /locus_tag="KPH93_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG1688875.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4440..4949)
                     /locus_tag="KPH93_002056"
     CDS             complement(4440..4949)
                     /locus_tag="KPH93_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG1688876.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5156..5374
                     /locus_tag="KPH93_002057"
     CDS             5156..5374
                     /locus_tag="KPH93_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1688877.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5392..7338
                     /gene="feoB"
                     /locus_tag="KPH93_002058"
     CDS             5392..7338
                     /gene="feoB"
                     /locus_tag="KPH93_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG1688878.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTHKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSNMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEESNKKSNTH"
     gene            complement(7498..8553)
                     /locus_tag="KPH93_002059"
     CDS             complement(7498..8553)
                     /locus_tag="KPH93_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1688879.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8601..9401)
                     /locus_tag="KPH93_002060"
     CDS             complement(8601..9401)
                     /locus_tag="KPH93_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1688880.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9394..10185)
                     /locus_tag="KPH93_002061"
     CDS             complement(9394..10185)
                     /locus_tag="KPH93_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1688881.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGILLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10225..10647)
                     /locus_tag="KPH93_002062"
     CDS             complement(10225..10647)
                     /locus_tag="KPH93_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG1688882.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10663..11142)
                     /locus_tag="KPH93_002063"
     CDS             complement(10663..11142)
                     /locus_tag="KPH93_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1688883.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11274..14030)
                     /locus_tag="KPH93_002064"
     CDS             complement(11274..14030)
                     /locus_tag="KPH93_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1688884.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVIAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4595 a         2443 c         1615 g         5539 t
ORIGIN      
        1 tggctacttt aaaagacaaa atttcaccta tcaataaaaa tcttaacgac taatactctt
       61 attttcaaat tttgcaacat ttttgttaat tttaaaacaa aaaaagacta tgcaatataa
      121 aaattatatt catagccggt tgcaccacta actccatata tctccagatg cccaataata
      181 agatatactt ctcagtttct aaaatatttt tattaaaaat cttttatata ttttcataaa
      241 tttctttttt attctaacat tgtaatatta ttttgtcaat atttccatat ttttagatat
      301 aagatactaa aatatctatt ttatctaaag atatttatat ttctacattc aataatagac
      361 aataccctgc aagttaaagc atcattttta tgagcttatc ccacagggta ttatatgttt
      421 actttatatt ataatttatg ttattgatta actttaataa atcctttttc tgaaacaagt
      481 gcttgtcctt catcacttaa tatataatct attaaagctt tacctttttc tgacaaacca
      541 tcttctttat taactaatat aaatggtctt gatattggat atttattagc tattacattt
      601 gcatcagttg cttcaacatc atttattttt aaactattta ctttctcatc aacatatcca
      661 aatgatatat atcctatagc attttcattt ccttcaactg tagtttttat atttcctgaa
      721 ccatcactta tttgtgcatc tttagttagt tcttctgaat caaaccctac aatttcttgg
      781 aatccatctc tagttcctga accttcttct cttgatacta ttactatcgg tgcatcattt
      841 cctcctactt ctttccagtt agtaatcttt cctgtatata tacctttaac ttggtccatt
      901 gtcagatttt ttactgggtt tgcagtatta gttatcaatg ctataccatc tattgctatt
      961 ttagtttcct ttaatccagc acttttttct tcatctttta aatctcttga actcattcct
     1021 atttcagatg ttccttctat agcatttttt atacctccag aagaaccaat ctgttgtact
     1081 tctacactaa cacctttatt tttttcttga aacttttctc cgatagtttc cataagagga
     1141 cctattgaag ttgaacctga aacagaaact ttgcttgaat ctcctgaatc acctgaacct
     1201 cctgtgttag aaccacatcc tactgctaaa gctccaacta acattgtact agctaaaaga
     1261 acgcctattt tctttttaaa cattttactt acccccgctt atattttaat tttttgttgt
     1321 catttttcta tattttaata atacagattc tttgttaaat gaatattaga gcagggttaa
     1381 gtaaatgtaa atattgttaa atacatgtaa aaacttaatt ttatatgatt tattcttaat
     1441 tagattaact ttttaaagtt actttaggca gatataaaca taatacaact catattatca
     1501 gaagtcttca ttgaagctat caacctatca catcctataa ttctttttct agtttattaa
     1561 ccaattcagc aaatacattt aaggcttcct ctatagattc ttttttcgtc atatctacac
     1621 cagcagattt taattgttct agtgggtatt ctgaccctcc actttttaag aagtttatat
     1681 atctatctac tgcaatactt ccttctgaaa gtatctgttg acttaaagca cttgcagcag
     1741 aaaaccctgt ggcatactta tatacataga agtttgaata aaaatgaggg attctagccc
     1801 attctattcc tatttgttta tctactattg cagattttcc gtaatacttt tcatttaatt
     1861 tgtagtatat attagtaaat tcatcagcag taagtggttc tccactctct accctttgat
     1921 gtacaatttt ttcaaattca gcaaacatag tttgtctatg aactgtagtt ctaaattgtt
     1981 ctaaataata atttaataag tatatagttt catccttaga cttagaattt tctaataaat
     2041 aatttataag aagtaattca tttaatgtag atgcaacttc tgcaacgaat atcttatagt
     2101 ccgaatataa aaatggttga cttgttcttg aataatgact gtgaacagag tgccctaatt
     2161 catgaattaa agtgaataga gaattaaggt catttttata gctcattaat atataaggat
     2221 gtgaatcata acttccccaa gaataagctc ctcctttttt accttcatta tcatatacat
     2281 caatccaccc ttcatcaaaa gcacttttta tgaccttcaa gtattcttcg cctaaaggtt
     2341 tgagtgcctc taatatgata tcctgtgctt tatcatatgg tatatccatg tcaaatttat
     2401 ctgtcaaagg aacatacaaa tcatacatgt gtatttcatt caatcctaaa aatttctttt
     2461 taagctcaac atatttattt agattaggaa tacttttatc tatagctgat ataagattat
     2521 tgtaaacatc taaacttaca tcatctgaaa ataaagacgc ttctattgct gaattatact
     2581 ttctagtttt tgcgtaaaat atctctgatt ttattccacc ataaagcgtt gaggcaaatg
     2641 tatttttata tttctcatat gttgaaaatt caccttcaaa agcgtctttt ctaactctac
     2701 tattcttact ttttataaat aaagagtaat ttgaatgaga aagcttaacc ttattaccct
     2761 cttcatcttc tatctcagga aattccatat ctgcatatgc taacatatcg tatacatttt
     2821 ctggaattgt cgataattct gatgtagctg caagtatttc ttcttctcgt tcactcaaag
     2881 tatgtggttt atctctcaat atatccttta tatattttgt ataaaaagat agcttctcat
     2941 cttttaaata ttcacttaac ttattttctt ctattgccag tatttctgga actatataag
     3001 aagtagccac acctaaatca gtagataaca tttctgtctt agtggctctt ccttgattat
     3061 cattaattct agtatcttca tgttgtttca tatgagtata tacatataaa ttttgaacaa
     3121 tcctagatgc attttcagag atatttaata ctttatataa gttttccttg ctctcagaaa
     3181 gtttcccttt atattctttt acttcttgga taagattttt tactttgctt atgtcttttt
     3241 caatttcctc tttactggag tacatcttat ctatcttcca tttatacttt tcttctattg
     3301 tacttctatc agttcccata tcatacacct ctattttaat ttgttttata ttttattcac
     3361 tttacaaaat atattgtata tattttagtc caaataatct acatttatta ctccttctat
     3421 catatttatt tctttcataa cgtcgtgtaa gtgtattctt ttagcataag atacaaaaaa
     3481 attaaccttc cagtattctt cattacgaca ttctatttcc atatttttta cgataagttg
     3541 atttttttca aatttatcat ataaaaaagg tatagagtct aaattgctta attttaatat
     3601 caatacattc ttttcttggc taaataaaaa tgtattgatt atcctaagca caaataatgc
     3661 tatcattaca aataatccac ctagaattgc caattcataa taaccatatc caactgctat
     3721 accaatacat gcagttgccc acatcccagc agctgtagtg agccctttta ctccaccttt
     3781 attttttaaa attgctcctg cacctaaaaa accaattcca gataatactt gagcaggcat
     3841 tcttgctggg tcagcatttg tatacaaatt ataatcaaag aataattgaa tcgaagttat
     3901 tgaaacaata caggaaccta ttgcaattag tatatgtgtc ctaaatcccg caaactgtct
     3961 tattttttct ctctctaagc ctattattcc tcctattatt agtgctaata gtaaccttat
     4021 tgctatctcc ttacttccta tatcaatcat aatataaaac cctcctgact taattacaac
     4081 ttatgtccta cagataattt acacatcaga aatactattt tatatcttaa tattattatt
     4141 aatatatgta aaatatagcc atctgttaat ttttgataca aaaaaaccca caactgtggg
     4201 ctatttgctt aggtacatgt atttatttat taatatatct attttcttac ttgattctac
     4261 taactcttct tgaaatattt ctggatattc tatgtatgta tttatctctt ccctaactgc
     4321 ttcaatctca ttttttaacg cttctaattc attgttcgcc ataataatca ccactattac
     4381 aaatttgtat atttttatat acattttatc aaacaatatt atattttgtc aatattatgt
     4441 caataaaact tactataaaa atgttttaat ttaggtattg tatacctgga tactaaacct
     4501 acaaacatac cagttactaa tgatgccatt aacataaatg gtaagtaccc tatagcaagt
     4561 atattttcta tgattaaact tgcaactatt aattgaccta tattatgccc tattgcacct
     4621 ataatgctta ttcctattag tgataaatct tttattttac tcgctaaata cataaataaa
     4681 agactcagat aagctcctcc tatactaaat agtagatatg ataaagggcc tgcaaatatt
     4741 gatgacaaaa ttaccctgat agtaacaatt ataacagtat ctttgacacc taacaacatt
     4801 aatgaaatta aagttataat attgctcaaa cccaattttg cccctggaaa tataactata
     4861 agaggatttg gtatatatgt ctctagtaca tataaagcta aactataccc tatcataaga
     4921 cctaaaaatg tcatttttct agttttcatt aagttaactc ctccaaattt tatctatcat
     4981 cttacattat aatacaaaat atataaaaac acctaattta gaccaaaata ttttttaatc
     5041 tatttagaat aaatattagt tatttcaatg tgtaattgtc tctataattc ttgacttttt
     5101 gtatcatact tgttacaatt ttaatgataa tgaatttcat taggaggtgt taataatgac
     5161 tgtttacgat ttaaaaatag gagaaaaagg atttattaat agtatagatg gagatattaa
     5221 attatctaaa agattgcttg cacttggatg tataaatagt actgaaatag aagttaaaaa
     5281 ggttgcccca cttggagacc caattataat tagatttaga ggctttgacc ttgcgattag
     5341 aaaatctgat gctaaaaata tttctttaca ataatagagg aggagctacc cgtgattaat
     5401 gtagcattat taggaaaccc aaatgtcggt aaaactaccg tttttaatct acttacaggt
     5461 tcaaatcaat atgtaggtaa ctggcctggt gttactatag aaaaaaaaga aggatttctt
     5521 ggaaaagaga taaaggttgt cgatttacct ggtatatatg caatggatac tttctcaaat
     5581 gaagaaaagg tttctaaatc atatttagaa aatgaagatg ttgatgtaat agtaaatgta
     5641 gttgatgctt ctaatttatc aagaaacctt tacttaacaa ctcaacttat gcaatttaac
     5701 aaacctatag ttatattgct taatatgtta gacattgcag aatctaaagg agttaatata
     5761 gatgctaaaa aattaagtga agaacttgga gtaattgttg ttccaattat agcaaaaaag
     5821 aaaaatggtg ttgataggat agaagaagag attaagggag ctacacaaaa agcttttata
     5881 tatcagaaaa actttggaag tgaaacagaa acatataaaa aattagaatc cattttatcg
     5941 agatgtacaa aaactacata tacacataag aaatctatca gtgataaaat agataatata
     6001 gttttgaatc ctatattagc atatccgata tttattggtg tattgttcct cttatttaaa
     6061 tttacctttg attgggtcgg aggaccacta caagagggat ttgctagtct tatagaggct
     6121 tatatttctg cacctgttag taatatgcta tctaactcta gcccatggtt taaatcactt
     6181 atagttgatg gaataattgg aggtgttggt ggtactttac cattcttccc tttaatattt
     6241 acattattct ttggtatatc gttgtttgaa gatagtggat acatgtcaag aacagcattt
     6301 ttaatggata aagttatgag aaaggttggt ctatctggta aagcttttat acctatagtt
     6361 atgggtatgg gatgttcttc tccagctata atggcaacta gaacattgga aagcgaaaaa
     6421 gatagaagag tcacagctct tattgcacca cttatgactt gtggagcaaa gctacctata
     6481 tatgcactat ttgtagctat attctttcca cataatgctg ctttagtaac tacgtcactg
     6541 tatttggtgg gtatagttat ggcaattgta gttgcattat tcttaaataa aacagccttt
     6601 aaaacagagg ctgaaccatt tatattagaa ttaccagaat atagaatccc tactatagat
     6661 gcacttatta aaaatacttg gaataaatca aaaggtttct tgataagagt tgttactgtc
     6721 atgtttgcaa tgtcagtcgt aatttgggga ctttcttcat tcaatgtttt tggatataca
     6781 gaagatataa atgtaagctt cttggctact ctaggtcata ttatatctcc tatattcaaa
     6841 cctcttggtt ttgatgattg gagaacttca gttgctattt taagtggtct tggagcaaaa
     6901 gaaatcgtag taaactcttt aaatatacta tatggtaatc taactgttgt cttaccaaca
     6961 atatttaatg gagttactgc atatacattc ttgatattta ctgctttata tactccatgt
     7021 atagctgcac ttgcaactat gaaaaaagaa tatggcaata aaatgatgtt tacatcattt
     7081 gcgtatcaat ttatactagc atggataatg gcttttatag ttaaaagtat aggtggagta
     7141 ctatttatgg gtaattcaat aatggaagcc ttgataggtg gaataattat agtccttgca
     7201 ctctttattc tgtttaataa attcaagtct acaaaaaacg gaaatgtctg tagtggatgt
     7261 tctatttgct ctggatgtcc aagtgctaat tcttgctcaa gtaaggaaga aagcaataaa
     7321 aaaagtaata cacactaata tataaatatt ttagtttaaa ataagataaa agtgggtgtc
     7381 tcaaaattaa ctttttagtt catgaggcac tcttttttat acgaaatcaa atatattttc
     7441 atcatttcaa cagccaaaaa gaggcttatc tctattttga gacaacctct ttttttgtta
     7501 cttattcagt ttcttgctac ctaacttcat gtggaaccta gtatcttttg gaacacttgg
     7561 gtctacgcct cttaatgtag gtatttcccc acatattagt tgtataggta ctataaaatt
     7621 aaatgtttga tagtaaggat tatctgtgat atcaatcttt aaatttttat cattttctgt
     7681 aacatctcta cctatagcaa atacattttc cgtccaacca gaaagtacat caatcatctt
     7741 agttactcta ggttcttttc ctgtatctag tataaatata gtagaatcac tatttatagc
     7801 attatatata ccatgtataa actcttcaaa ctcataccca gttacaggta ttctcattgt
     7861 ttccaataat tttaatgctg cttctaatgt atctccatat atatcagagt gacctattat
     7921 tcttatttct ttagaattaa ctaacttttc tttgtttctt tcaatccatt gtttagatag
     7981 cttatacact gcttcaaatc tattaattgc atctaatatc ttatttattt cctcattata
     8041 tttttcacta gatattatac ctttttctct agctatttga agacctaata acattaagtt
     8101 taactttgta caataatatc cttttgtttt agctccagat ttttcttcac cacaatttac
     8161 tgttaatata taatcagaaa tctcatctat tagagcattc ttacaacctg ccatagatgc
     8221 tattttacaa cccttatctt cagctaactt catagcgttg tatgtagaat aactactccc
     8281 accttgagat acacctacta ctagggtatt ttcattatca aacttgaatg tatcttcagt
     8341 tatcataaat ggatacattt tcacaacatc tatatctaat aaattttgta aatatggttg
     8401 cacttgcact cctgagtgat agctagtacc tgaacctgtt attataatct tttttagatt
     8461 tgttctctta acttcattaa ataagctatc tgcattagat ataatttctc tcattctaac
     8521 tggtgtttct aacatataat cttgtattgt cattttatac accttcctta tctatatatt
     8581 caataaatta taattaattt ctataacaaa cctatagcat gtaaagcgat tcctgttact
     8641 atagtgccta tagttaattt tgttacactt acctttctct ttaacatgta gtatattaca
     8701 aatgcaaagg ttaaaggtaa catattaggt attattttat ctagtatatc ttgtaaaatt
     8761 acttctgcac catttaagtt gaatttcaga ggagtagtta tactcaacat agttgcaacc
     8821 attcctccta caacgaataa accaacagtt gtagccatag acattatttt ctccataagt
     8881 ccctctcttt gtattctttc aagagaattt acccctactt gataaccata cttaagtcca
     8941 aatattctaa gggcaaatgc tggtatattg tataatagta agaataatag tggtccaaag
     9001 atattacctt cttttgcaag tgatactcct actcctgctg ctataatcct aaatgttccc
     9061 cagaataatg aatcaccaat tcctgcaaga ggtcccataa gagctgtttt aacagcacta
     9121 attgaacttt catcaaattc tggattatta gcattctctt cttccatagc tgctgttata
     9181 cctaatatag caggaactac tactgtcgtt gtattaaaaa tctccaaatg cctttctatt
     9241 gcttttgctt gattttcttt cttattgtat agcttcttta ttgcaggtaa cattgaataa
     9301 cagtaaccta agttttgcat tctttcgtaa ttaaatgcac cttgaagagc aaatgaacgc
     9361 caaaacatac ttcttaattc tttttttgtt aatttattca aagtcaatct ctccttccgt
     9421 taaagtattg attgctaatt gaggattttg attattttct ttgttattga tataaaagtt
     9481 tagtacaaat gctacacata ctccaaataa agctatagca gttatatcta atccaccata
     9541 agatgctaac aaaaacccta taaagaaaaa tacagacatc ttttttgaaa gtaacatatc
     9601 cataagcaat gcaaaaccta ctgctggcaa caatattcct gcaacaccca gtccgtctaa
     9661 tataacttta ggtattgcat ttatcaatgc tgtaacttta tcagcaccaa tcataattgc
     9721 taaaaaggtt ggaactgatg tacttaaaaa gaataatatt actggcaacc acatcattat
     9781 ggtaactttc ttaaaatcag cctgtttagc atagaaaact gccttttgag aaaagtacga
     9841 atttattatt ctaactaata ctccaagaga ttgtgctaag actgctactg gtactgctac
     9901 tgctaaagca acttctgcac cattacctga taatatagca aaagctgtac ctaatacacc
     9961 tcccgttact acatctggag gagtagctgc acctattcct gcaattccca tccaaattaa
    10021 ttcaagctgt gcaccaatca taattcctga ttttatgtct cctaaaacta atcctactaa
    10081 catacctgta actattggcc tatcaagcat agattgacca aatatacgcc catctagtat
    10141 accaactcct gctataatag ctaacatagt agcttgtact aacatattga acctccattt
    10201 taacttagtt taatttacat taaattacat tacatttttg attttgtttt gattttctaa
    10261 taactcattt acataaacaa ttccgttttt tgcttcctct aatcctgatt taactattag
    10321 ttttgtatcc tcctcaacta ataaaagtat agataaaagc attggtaaat ttactcctgt
    10381 caataattca aatttgaacc cttgtaaaag taagtttgta catatgtttg ccacacttcc
    10441 accaaacaca tctgttaaga caacaaattc ttcactatct ttattatttg ataatatact
    10501 tctaatttct ttctcagcat catctgctga tttatccgtt gtcatacaaa aatactcaat
    10561 attagttaat gaaccaatta ttaatttact tgtgtcaaca agctctctgg acaattctcc
    10621 atgtgttgca attagaaatt ttttcattct ctttctccta aattatttta ttaaatctga
    10681 tagttgtttc tttttgtctc taggtaacat tctaaattct atatcaatcc ctaaatcacc
    10741 tatttgttta aagttattta tatcatcatc atcaacagca actaaatctg taagtttctt
    10801 cttcccttct tcatatctta atcctcctac atttatttct tttaatactc ctcctgaatt
    10861 tttagctaca actaaagcat ctttggtatt ttttactaac actaatacat tgcttttttt
    10921 tgaagaagca aatttttcta ctatttctcc agattcctct acccccctta tatataaagt
    10981 tacattgcta ggtttcgcta agttcagtga catagctttc atttggtcat tttttgcttc
    11041 atcatttgca actagtatta catttatacc tagtgatgtt gcccatccat atgcaacctg
    11101 accatgtatc aatctgtcat caattctcat caaagatacc attttcccca aatcctccat
    11161 aatacattaa aatttttatt gtttatactc tcatttatct ataaaagcaa aaagtatgcc
    11221 aaatactggc atactttttt aaatttcatt cacttatttt gtaatcttgt attttactgt
    11281 ggtagaccat agataatatt ataaatatat cctatctctg aaattggtat ttgcacacta
    11341 taactctttt ctagcacact aaatgcattt tttattagtt ttacttcact atgatggttt
    11401 ttgattaaca aatccatatc agtatactct tcaatttcag tttgtctaac taatctttca
    11461 atcatacaac ttaagtgaac atataaagaa atctttttat catttgaaag ttttttcttt
    11521 tgaatatttt ctatagcatt aattccttct tctattctct ctattatctt ttttgaatct
    11581 aaaatagtta gactatctat tactctaatt aatgaaaaat tctttacaat agaatcattt
    11641 atgttcttta ttttttcatt ttctactact ctgctaaata ttctaaaaac atcatcttct
    11701 ccttttcctg atattatatc ctctaataat ataaaatcta ccccattcac atttggattg
    11761 gcagttccta ctatagcaat cacatcatac atttcaaaaa ctggttcttt atctcccata
    11821 gagacaagac tatcataatc ataaggtatt attttgacat caactattcc aattaaactt
    11881 tcggaaagta gatgttggat ttttatagca gtacctattc ctgtaaaaca agatgatatt
    11941 atagcatatt ctttattttt ttgaggataa tacaattgta tattaggttt agtttcttca
    12001 tccataattt ctgcaatctc ttcaatgttt ttaccttgca ttaataaatt tcctacttct
    12061 agtgcagaaa tagtggatat ttggtctata aataacattg ggctatttat cttattcttt
    12121 aaatcttcat atataagatt aagagagccc atatcaaata atacaattaa accattcttt
    12181 atcctatctc cctctaggta tctagacatt tgttttgata catcagcaat agtagcatct
    12241 ataggcatat caatagctgt aaatatattt ttacccaaca tccgattaca tacattcgcc
    12301 atactactgg cagtagcata tccatgtgct ataatagtcg cattaacttg attaaatttc
    12361 attcttataa ttgttgattt aataaatata atcattatga ttgtatctac attattaaga
    12421 tacatatcca atctattcgt tattaatgtt gatattttac ttattataga gtattctttg
    12481 taactctctt tttttaaata tattagtaat ttattttcta aagctttata ctcatcagac
    12541 aattgataag attcgtcctt caaataaaga taagacgaaa atgccaatac attattccca
    12601 tcgtatttta tattgttatt aacttctaaa tttctaaata tatcctgcat tattgatatt
    12661 ataagctcca taagtacgtt attattttca tcattatttg aaaagactaa attgtccatt
    12721 agtctaatca ttagattaga actcttttta ctaaattttt cctttgttat ttcatttttt
    12781 tctagtctat caaagtaatc taaacatttt atgaaacttt gatagattat atttctttta
    12841 tttcttccat ttgacatttc aatactaaat tgtttaggag aaaagataat gcttttgctt
    12901 ttaccacttt ggaattgaaa tatatctaca ttttctatat aaagatttct aggaaggtct
    12961 tgaagagtaa caaaaatatt tccatttctt ctttgatttt ttgcataagc tcgtccacat
    13021 gcatatttta ttacattttt acattcacct atattcccct tgaattttat ctttaaaatc
    13081 gcatttaatg taatttgagt tatctctata ggcaaaccaa gtaccttact ttcttcataa
    13141 ataaaatgtt ctacaatctt tcttttttca atagtccctc tttcttctat attgggaaca
    13201 taaatattta tgggtattct tcttaaaaag gtttgtaaaa attcagatag actttctgtt
    13261 gtagcaaata ttaatcttac cctagctttt ctaattactc cattctctcc aatcctagaa
    13321 aacgtacctg tatccataaa tgtaaataac ttttcctgac cttcactatt taatctatgt
    13381 acttcatcta aaaataaaac accaccatct gattgttcta ttagacctgc tctagattca
    13441 tttgctccag tatatgctcc ttttacatgc ccaaataata gactagacaa taactccaaa
    13501 ttattataat actgcgcaca gtttaacaca ttgtatggag aatttttttc taatacatta
    13561 ctgcttacac tatatttgta tattatcttg gctaaaaaag tttttcctac tccactttca
    13621 cctgtcagca ttattgtaag tccgccattt ggataaagta cagcggtttt tatttgttcg
    13681 attacttctt ttaagctatt atcatatcct atgatactat taaacacagt ctcttgcttt
    13741 aataatttac actcattttt aagttcatct aaattttcat aaatactttt ttttgtaggt
    13801 cctactacat catttatttt atttttaggt aaaaaaataa caggtctact gtttatcttt
    13861 ataagattat cagattttac taaatcattt aataatctac ttgattgagt tctacttaaa
    13921 ttaaactttt ttactatatc ttctgttatt atgccacttt cttttttaag ttcatcaaag
    13981 ttgttaaata atacacattc atttacaaaa cttaaaattt catccctcat gttcaacctc
    14041 caatacaaat atcaataata ctataatatt agtatatcat ttatatattt atgaaaaaga
    14101 taattatatt cctatcttga ttctcttttg taaggttgta aaatcaaact ttttataagt
    14161 ataaaatcta gattttttaa cataatctca ag
//
LOCUS       DAEMAE010000088        14159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEMAE010000088 DAEMAE010000000
VERSION     DAEMAE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.090"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..437
                     /locus_tag="KPH93_002065"
     CDS             <1..437
                     /locus_tag="KPH93_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688885.1"
                     /translation="DKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKSINE
                     LEDYISQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDKNKL
                     EMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            452..1036
                     /locus_tag="KPH93_002066"
     CDS             452..1036
                     /locus_tag="KPH93_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688886.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLISLKNEKQNI
                     INKINNQDNYQIKDNKRIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDSQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1289..2326
                     /locus_tag="KPH93_002067"
     CDS             1289..2326
                     /locus_tag="KPH93_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG1688887.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2407..2772
                     /locus_tag="KPH93_002068"
     CDS             2407..2772
                     /locus_tag="KPH93_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688888.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2777..3094
                     /locus_tag="KPH93_002069"
     CDS             2777..3094
                     /locus_tag="KPH93_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688889.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEIILGSEKLYVCELRVVLI
                     KVNSLYVERLNKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3142..4746
                     /locus_tag="KPH93_002070"
     CDS             3142..4746
                     /locus_tag="KPH93_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688890.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPIGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDILVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4870..6498
                     /locus_tag="KPH93_002071"
     CDS             4870..6498
                     /locus_tag="KPH93_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG1688891.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6763..8112
                     /locus_tag="KPH93_002072"
     CDS             6763..8112
                     /locus_tag="KPH93_002072"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG1688892.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDSSKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYISEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8268..9347
                     /locus_tag="KPH93_002073"
     CDS             8268..9347
                     /locus_tag="KPH93_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG1688893.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKTGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9692..10600
                     /locus_tag="KPH93_002074"
     CDS             9692..10600
                     /locus_tag="KPH93_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG1688894.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10605..12974
                     /locus_tag="KPH93_002075"
     CDS             10605..12974
                     /locus_tag="KPH93_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG1688895.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLRELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDVNFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13299..14102
                     /gene="proC"
                     /locus_tag="KPH93_002076"
     CDS             13299..14102
                     /gene="proC"
                     /locus_tag="KPH93_002076"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG1688896.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSNVAVTNSDVVIVALKPNICEKALTPLKGLIDVNKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5803 a         1468 c         2601 g         4287 t
ORIGIN      
        1 tggataaaac aataaatttt cttaatggat ttaaaaaaac taaaccatgt aataaaatat
       61 ataaaataat aatagtctta attttaacgt gtattttatt taaatcattt atgtatgtga
      121 aatctataaa tgaacttgaa gattatataa gtcaaaataa gctagtggat aaatcaaagc
      181 acactttacc aacaacagat aaaactagag agaaattttc taaaataaaa cttaatgaaa
      241 taaaaaaaat atatgaaaca attggaataa atagtattac taaaatatcg atagataaaa
      301 ataagctaga gatggaaggt gtgtgtagtg atttagatgt tttaaacaat ctaaggaaaa
      361 taaagtacat aaaatattat tacatagaca gtattgtaag agatggagaa tattatagat
      421 ttagaataaa aagttagatg ggtgattatt tttgagatta aataatagag aaaaaaatct
      481 acttaaaata ttgttaatat tagttattat attggcaagt gatatagcgg taagttattt
      541 ggaagaaaac ctaattagtt tgaagaatga aaagcaaaat ataataaata aaataaataa
      601 tcaagataat tatcaaataa aggataataa gcgtatagat gataaatata tagaaagtgt
      661 tgaattaaat aaaaatgaag aattgaaaaa taattttaat aactcatttt tagataaaaa
      721 aacaaataaa gatatagtgt tattagtaga aaatgaactg agagatattg tgaatgtaga
      781 atatattaat caagaaagta gtttagataa tcacaataaa aaacaagtta atttagaaat
      841 aaaagttaat ggagaacttg caaatgtgtt gaaacttgaa ggtgtaatga agaagttaag
      901 actaaataat aagcttgaaa agatagaaat taacaaatta aaagtcaata attttgattc
      961 tcaaaacatt ccaaatgaat ataaacaaga aaaagacaat attgttgaat gtaaaatgac
     1021 agttagagtc atataacaaa aaagaataga taaagtaaaa aactattatc aattaataaa
     1081 attttataga ggaattagtt ttacgataat tcatttctac aagagggatt gctgatgatt
     1141 ttatcgaaat cctaattaaa gttaatatat tgtaccaaaa tataagtgaa tttaatagaa
     1201 tatttactta tactatagat tgattaataa aaaaatggta taataacaaa ttgtacttta
     1261 atttaataat aagggagtgg aagtcatatt gttagggaga aatgagctat gcccttgtgg
     1321 aagtggcaaa aaatacaaga aatgttgttt aaataaagat attgtttcag aaagagcatg
     1381 cagaaaaact gaactttcac aaaaacaata tacagattta tattcaaagt tatatgatta
     1441 ttcaagacag gataagttta aagaagaatg tgaaaaagct aaagaaatgt tttatataat
     1501 gcaaaatgat agtgtaaatg aaaaatttga aagatttttt aatacttatt taatacaaga
     1561 ccacataatg gaaaataaaa aggttatgac agtcggtttt cttgaagaaa atggtgcaaa
     1621 cctaagtcaa tcagaagtat ccatattaaa aagtatgttt gaatcatacg taagtattta
     1681 tgaggtaaaa gaagtttctg ctggaaaaat agtattaaag gactgtttaa gtggaaatga
     1741 actttgtaca gaagatgtaa aacttctaag aagttttaaa atcggaagtt gtatgatagc
     1801 taggatagtt gatattgaag gaactaacat attgatagat ataactataa gtatatcaaa
     1861 tgaagttaaa gatataattt taaaggatat tatgaatcta ttcaatcaat atcaagatgt
     1921 atacaaagat atgaagtcgt tcttaattta tcatacacat atactataca agtatataca
     1981 acagctttta gacccaagta tagctgaata cttaaagaga gaaagagata aaaacgatac
     2041 aaaagttaat tcagaaaatg aattagctga agaagaatgt aaagttttag atactcttaa
     2101 agaaaatgta gaaaaagaaa atatagatgc atgtatagag ttttggaatg aatataagaa
     2161 aaatcatgaa aatataaaag gcgctgaaaa tggttgggca gctgctgttg aatactatgt
     2221 taaaaaagaa gcaggacaac ctataactca agttcaaata tcaaagaaat acgatataag
     2281 ctctagtaca ctaggaaaga gatacaaaga tttaaaaata tcttaattaa ttataaaaag
     2341 ctttaagtca tagataaaga atctatagta taatatatgt ataatgttta gaaagaggta
     2401 ttaataatga gtcttagttt aggaaatgca attgctgtat caacgaactt tataaaatct
     2461 aacccagctg tacctgtgta caacggagaa gaaatatatg cagttattta tcatatagaa
     2521 ataggatata ataatggtgt atcacctgat accaaacaat atataataat agactataaa
     2581 gagtttgatt tatctatgga taaaaaaaat gaactaataa atattgctaa agagtactgt
     2641 aaatctaaag gtattgtaga aaacgaaaac cttgaaataa accttttaga taatgaagaa
     2701 gcagagaact tcttagaaaa ggtgtttaat aacatgaagg ttataagagg tcttataact
     2761 aaaaaaggat aaataaatgg actatatagg aatagaaaat ataacacctt atgaaaatac
     2821 atatgaattt agtgtatatg aatatgatga tgaaatcatc ttaggtagtg aaaagttata
     2881 tgtgtgtgaa ttaagggttg tattgattaa agttaattct ctgtatgttg aaagattgaa
     2941 taaatcagtt gaagcaatgg tcttagtaaa aaacttgaaa aaagatttag ataaaacact
     3001 tgttgtaaac aaaataaaga attttgtgct agatgagatt tgggtagaaa atctagtaaa
     3061 agagaatata gaagttatat ttgtagaaag ctaggaataa ccctagcttt tttatattac
     3121 aatctaagag aggaaaacaa tatgttaaga gtatctaatg taaaacttgg aattgataaa
     3181 gatatatctt tattaaaaga tgttgtactt aagaagctaa aaataaaaga aaaaaactta
     3241 gtcaaatata gtatatataa agaatctata gatgctagaa aaaaaggaaa aatggaattt
     3301 gtttattctg tagatgtaga agttaaagat gaaaataaaa tacttaactc aaaaataaaa
     3361 gatgtaacaa aaataaagga aatcagatat gtaaatgtac ccataggtga taaaaaattg
     3421 aagaataatc ctttggtaat tggtagtgga ccagctggtt tgtttgcagg tctgctttta
     3481 gctcaaatgg gttatgaacc aataatactt gaaagaggtc ttgatgtaga cagtagaacc
     3541 aaagatataa gtgatttttg gacaactaga aaattaaaaa ataattcaaa cgttcaattt
     3601 ggagaaggtg gagcaggaac tttttcggat gggaaactta caactagaat aaaagatata
     3661 cgatgtagaa aagtattaga agagttagtt cagtttggag caccagatga aatattatac
     3721 tcacataagc cacatgttgg aacagatata ttaaaaaatg tagttaaaaa tataagaaat
     3781 gaaataataa gtttaggtgg tcaagttaga tttaattcaa aagtaacaga tatacttgta
     3841 tcaaatgatt ctatacaatc tgtaataata aatgatactg aaaaaataga tacagataca
     3901 attatattag ctgttggaca tagtgcaaga gatacttatg aaatgttgta ttcaagaggt
     3961 ataaaaatag ttcaaaaacc ttttgcaata ggagctagag ttgaacatcc acaagattta
     4021 ataaataaag cacagtacaa ggatttttat aatcatccta ggttaggagc tgcagattat
     4081 agacttatag agcatgtttc aaatggaaga acagcatata ctttttgcat gtgtccagga
     4141 ggtagtgtca tagcatctgc atcaaatgat tttgaaatag ttacaaacgg tatgagtgaa
     4201 catgcaagag ataaaataaa tgcaaatagt gcatttttag taaatgtaac gccagaagat
     4261 tttaagagtg ataatccact agcaggtgta catttccagc agaagtacga aaaattagct
     4321 tttgaattag gtggaaaaaa ttataatgca ccagttcaac ttgttggaga ttttataaac
     4381 aataagactt ctttaaattt aggaagtgtt gagccatcat atagaccagg atatacactt
     4441 acaaatttaa gtgaatgttt gcctagcttt gtaacagaga ctatgagaga agcatttata
     4501 tctcttgata aaaaattgaa tggatttgca atgtatgatg ctgttttgac tggagtagaa
     4561 actaggtcat catcaccaat aagaatagta agagatgaaa atactttaga atcagtgagt
     4621 attaagaatc tatatccatg tggtgaaggt gctggatatg caggtggtat agttacagca
     4681 gcagttgatg gtataaaatg tgcagaaaaa ataatacaaa aatacaccaa taatacaaaa
     4741 atttaacata aacttaatga ttttttaata tttaacatga atttaacatt atcactccta
     4801 taatttgata aaatacaata ggttataaaa tttttagata cagtaatctc tttacaagga
     4861 ggaaataatt tgaacattat aataagtcta atgggtggat taggtctgtt cttatatggt
     4921 atgaacctta tgggagaagg tcttcaaaaa tctgctggga caaagctaaa aaagataata
     4981 aaactactta ctagtaacct atttatggga gttttggtag gtacaggagt tacagcagta
     5041 atacaaagtt caagtgctac tactgtaatg gttgttggtt tcgtaaatgc aggaataatg
     5101 actttaaaac aggcaatagg agttataatg ggtgctaata tagggactac tgttacagcc
     5161 caattggtat ctttcgactt aactggtatg gccccagtag cattaggtgt aggtattata
     5221 ctttatttat ttggaaataa gcctagaata aaaaatatag ccgaaatact tataggtttt
     5281 ggtatattat ttacaggtat ggattttatg aagatggcag tggagccact aagagactat
     5341 caaggattta ctgacttact tgttacattt ggtagatatc cactacttgg attattattg
     5401 ggatttggga ttacagcaat aatacaaagt tcaagtgcat ctatgggtat gttggttgca
     5461 ctggcagcag aaggtctagt gccattatca gcagcactgc caattcttta tggccaaaat
     5521 attggtacat gtgtaacttc actgttatca agtattggtg ctaacaaaaa tgcacgaaga
     5581 gcagctatga tgcatctaat cttcaatgtt ttaggaacgg tgatattctt aatattcttg
     5641 aataaaccag tagtaagcat ggttacaagt tgggacccaa gcaatgttgc aagacaaata
     5701 gccaatacac atacattgtt taatataata tctgtattga tacttttacc atttacaaat
     5761 cttattataa aactagctat aaagcttgtt cctgatagag caggtgatat agatgaagat
     5821 gaaactaaaa ctattaaata tatagatgat agaatgatag aaactccttc tatagctctt
     5881 gcaaatacga taaaagaagc acttcgtatg ggtgagaagg ctaaggaaag cctaaatgca
     5941 tctatggaag ctttagtaga acattcaact gaaaaaatag ataagacata tagacgagag
     6001 agattaatca atgatttaca gaaagctatt ttaaattact tacttaaact ttcaaaagca
     6061 cctttagatg atgattcaag agaagttgtg gatactttgt ttaatacagt caatgatata
     6121 gaaagaattg gtgaccacgc taaaaatata gctgaattat cgcaagttgc aatagattca
     6181 aatatttctt tctcagaaga aggacaatct gaacttgatg ttatgtataa tagagttgtt
     6241 tcagcatata cttatgcatt agaatcaatg agaactgata atgttgattt agcttgcaag
     6301 gttataaaaa ttgaagaaca agttgatata atggagaaat cttgtagagc taaccatatg
     6361 tatagattga acaacaatct atgtagtata gaaaatggag ttatttattt agacgtaata
     6421 agtaatttag agagaatatc tgaccatgct gtcaatatag cacaacaagt tatagcgaag
     6481 agattaggaa atgattaaaa ataattaatt taaaataata taaaaattaa aatagaggat
     6541 aaatacaaga gaaactttaa atgatacatt tagagtttct ttttttgtaa atttatttga
     6601 ctaaaattta aaaatttata catagttata gtatttagaa gaatcgattt cctggtaata
     6661 aatgatgaga tatgtatgat tttgtggcaa acttttaaaa aaaacaccta tattttgtgt
     6721 tataatctct ttaaaacgaa tatgctgaaa agagggatta aaatgggaaa aataaatttt
     6781 gattatagta aagctacaga gtttttttgt caaaacgaaa tagatgtcat gcaaccatat
     6841 gtagatgttg cacatgacat gttacataat aaaactggtt taggaaatac ttttttagga
     6901 tggatagacc ttcctaaaaa ttatgataaa gaagagtttg acagaataaa aaaatctgct
     6961 gagaagataa aatcagactc agatgttctt ttagtaatag gtataggagg ctcttattta
     7021 ggttcaagag ctgctataga tatggtaagt cattctttta gaaatggact taaaaaagag
     7081 caaagaaagg ctccagaagt atattttgta ggtcataata taagctcaac atatattatg
     7141 gatttattag atataattga aggaaaggat atttctgtaa atgtaatatc taaatcagga
     7201 actactacag aaccagctct ggcttttaga atatttaaag attacttaga gaaaaagtat
     7261 ggtaaggaag aagcaagaaa gagaatttat gcaacaacag attcaagtaa aggtgcactt
     7321 agacagttag ctacagaaga aggatatgag acttttgtaa taccagatga tgtaggtggt
     7381 cgtttttctg tattgacagc agttggatta cttcctattg ctgctgctgg acttgatatt
     7441 gatgctatga tgaaaggtgc aaatgatgct agagaagcat ttcaaaatcc tgatttaaaa
     7501 agtaatgata gttatagata tgctgttgct agaacaattt tacatagaaa aggaaaagat
     7561 gtagagttat tggttaacta tgaacctcaa ctacattata taagtgagtg gtggaaacag
     7621 ctttatggag aaagtgaagg aaaagaaaat aaaggtttat tccctgcttc tgtagatttc
     7681 tcaacagatt tacattctat gggtcaatac atacaagatg gaaaaagact tttatttgag
     7741 acagtactta atgttgaaaa ctgtaagaga aatataacta taagttcaga ggaagtagac
     7801 ttagatggtt taaattattt agcaggtaag actgtagatt ttgtaaatca taaagctttt
     7861 gaaggaactc tattggctca tacagatgga aaagtaccta atttagtaat aaatatacct
     7921 caactagacg agtataattt tggatattta gtgtatttct ttgaaaaggc ttgtggaata
     7981 agtggatatt tattgggagt taatccattt gaccaaccag gagtagaagc ttacaaaaaa
     8041 aatatgtttg ctttactagg taaaccaggt tatgaaaaag aaaaggaaga gctagaaaaa
     8101 agacttaaat aattttaaat agttaaggtt taattaagat taaaataata taatgaaatc
     8161 atcaaaaagg agtttactca aaatagatga tttcattttt tttatgaaaa attgggaaaa
     8221 atatattttg ataatttata ttatattaag atagggagtg gtatgagatg tcaagaagaa
     8281 agaaagggat aagtttagta atattagttg ctataataag ttcaatactt agtagttttc
     8341 taacaataat tttagttaag gataatttag taagcaaatc tacaggaagt tctacgccga
     8401 tagttgtaaa tgatgatggt aagagtcaaa atatttatca agcagttgca gaaaaagcta
     8461 cgccatctgt tgtaggtatt acaacaacta gtgttgatac tagtaatatg tttgcaatac
     8521 caactgaaac acaaggtgta ggaactggaa tcatagttga ttcaaatgga tatatactta
     8581 ctaattctca tgttatatca gatggtcaag ctacaagtgt aaatgtatta ttcaatgatg
     8641 gttcaacaac ttcaggtaaa gtagtatggt ttgaccaaca acttgattta gctatagtta
     8701 aggttgataa aactggacta acaccagcag aatttgctga tagtgacaaa gtaaaagttg
     8761 gagatatatc aatagcaata ggtaatcctc ttggattaga ttttcaaaaa acagttactc
     8821 aagggataat aagtggatta gatagaacta tacaaacaga aaaaactaat atgactggtt
     8881 tattacaaac tgatgcaagt ataaatgcag gaaatagtgg aggaccattg ttaaatcaaa
     8941 aaggtcaagt aattggtata aacacagcaa aagcttctca agcagaagga ttaggatttg
     9001 caataccaat caatacagct aagagtatag ttgaagaagt aatcaaaact ggaaaatatg
     9061 aaaaagtaac tttgggtata aaaggtacag atgtatcaaa ttatgaagca gcaacaggta
     9121 caaaattaag tactgataaa ggtgtttatg ttgcagaagt aatatctggt tcatcagcag
     9181 aaaaagctgg agtaaaggta ggagatatta taactaaagt aggagacaca gatataacag
     9241 gtatgaatga tttaaataaa aagttgtata ctttctcaaa aggtgctagt acaaaaataa
     9301 cagtaaatag aggcggaaaa gcagtaacaa taaatgtgaa tttctaaata tgaccagaaa
     9361 gatggagact acatggtaaa gtgtagtctc tatttttatt taatttttat agtatatgca
     9421 tctaaattaa ttaaaatgat gtattgttta tagcttattg ctatattttc ttataaaaat
     9481 aaatttaata aatatttaaa tttaatttac aaaaacaata taaatatcat aataaaataa
     9541 aattttttaa tattactaaa aatatataga caaaataata acaaatatag tattatgtaa
     9601 ttgtagaaat gcttttcata ggtatattct atgaagataa attacataaa aatgaaaaaa
     9661 tggaatggat attatttagg ggtgaaaaat catgaatcca ttagttataa acttacaaaa
     9721 atgtagcata catgatggac cagggataag aagtacagta ttttttaaag ggtgtccact
     9781 tgaatgtgtt tggtgtcata atccagagag tcaaacatat acaaaacaag ttttgtataa
     9841 tgaagaaaga tgttcaaaat gtgaagcatg tataaatata tgccctcata aggcaattta
     9901 caaaggtgaa acaaaaatat gcctagacca agataagtgt gagttttgtg aaacttgctt
     9961 agactattgt gtaaacaatg caagagagat tgttggtcaa gagtattctg taagagactt
    10021 ggttaaagag atagagaagg atagaatttt ctatgaagaa tcaggtggtg gagtaacact
    10081 atcaggtgga gaagtaatgg ctcaagatat ggacttcatt tgtggagtaa taaacatgtg
    10141 caaatcaaag ggaatccatg tagcaattga tacatgtggc tatgcaaagt cagaaaatta
    10201 tgaaagagtt gctaaatgtg cagatttgtt tttatatgat attaagctta ttgatgaaga
    10261 taagcacata aaatttacag gaaaatcaaa tgacttaata ctgaaaaatg ttaagatttt
    10321 aagtgaatta ggggctaaca taaatataag aataccatta attgtaggag taaatgtaga
    10381 tgatgaaaat ttagaggtta aaaaaatgat agagttttta aagcctttga atatacaagc
    10441 agtaagtctt cttccatatc ataatattgg taaacacaag tatgataaaa tttataaaaa
    10501 gtatgaagga gaagaactac aaagaccatc agaagaaaaa ttagaggaaa taaaaagact
    10561 gtttgaagca agtaatttca atactaagat tggagggtaa taaaatggca agaggaactt
    10621 ttgagagaac taaaaaatta agagaagaaa gtattaatgc agaaccacac atatctattg
    10681 aaagagcagt acttatgaca gaagcttata aaaaatatga gggaagtgta gagattcctg
    10741 tacttcgtgc tttatcattt aagcattata ttgagaatag aactcttagc ataaatgatg
    10801 gagaattaat agtaggtgaa aaaggagatt ctcctaatgg agcaccaaca tatccagaaa
    10861 tttgttgtca tacaatggaa gatttagaag taatgcacaa tagagatata ataaatttta
    10921 gcgtatctga agaagctaga aaaatacata aagaagaaat aattcctttt tggaaaaaga
    10981 gacaaacaag agataagata ataaatgcaa tgacacctga gtggttagca gcatatgaag
    11041 cgggtatgtt tacagagttt atggaacaaa gagctccagg acatacagtt tgtggagata
    11101 caatatacaa aaaaggattt ttagatttaa agaaagacat agaagcaaga ttaagagagt
    11161 tagattttct taatgattta gatgcatata ataaaaaagc tgacttagaa gctatggcaa
    11221 tagcttgtga tgcaatggtt atattaggta aaagatatgc tgagaaagca agacaaatgg
    11281 cagaagaaga aacagatgaa gctaagaaaa aagatttatt actaattgct gaaacttgtg
    11341 atgttgtacc agctcacaaa ccagaaactt atcatcaagc tatacaaatg tattggtttg
    11401 ttcatatagg ggttactact gaacttaata tatgggatgc ttttactcca ggaagacttg
    11461 atcaacatct aaatcctttc tatgaaagag atgtagaaaa tggaatacta gatagagata
    11521 gagcacaaga attacttgaa tgtttatggg ttaaatttaa caatcagcca gcaccaccaa
    11581 aagttggtat aacattaaaa gaaagtagca catatacaga ttttgcaaat ataaacactg
    11641 gtggaataaa tccagatggt caagatggtg ttaatgaagt tagttatata atacttgatg
    11701 ttatggatga aatgaaattg atacaaccta gttcaaatgt tcaaataagt aaaaagactc
    11761 ctcaaaaatt cttaaagaga gcttgtgaaa tatcaagaaa aggatggggt caaccagcat
    11821 tttataatac tgaagcaata gttcaagagc ttatggaagc tggaaaaact atagaagatg
    11881 caagacttgg tggaaccagt ggttgtgttg aaactggatg ttttggtaaa gaagcatatg
    11941 ttctaactgg atatatgaac attccaaaaa tacttgaatt aacattaaat aatggatatg
    12001 acccaattag taaaaaacaa ataggtattg aaactggaga ccctagaaat ttcaaatctt
    12061 atgaggaact atttgaagca tttaaaaagc aattacatta tatgattgac ataaaaatag
    12121 aaggaaatgc tgtgatagaa aatatatgtg caaaacacat gccttgtcca cttatgtcta
    12181 caatagttga tgattgtata gaaaaaggca aggattacca aagaggtgga gcaagataca
    12241 acactagata tatacaaggt gttggtatag gaactataac agatagttta acagcaataa
    12301 aatataatgt atttgataag aaaaaattcg atatggatac attgcttaaa gcacttgatg
    12361 ttaattttga aggatatgaa gctatactta atttagtttc aaataagact cctaaatatg
    12421 gaaatgatga tgattatgct gatgaaataa tgcaagagat attcaatgca tattacaatg
    12481 aggtaactgg tagaccaact gtttgtggtg gagaatacag agtagatatg ttaccaacaa
    12541 cttgtcatat atactttgga gaaattatgg gagcaagtcc aaatggtaga ctttgtgcaa
    12601 aaccagtttc agagggaatt tcgcctgaaa aaggtggaga tactaatgga cctacagcag
    12661 ttataaaatc ttgtgctaag atggaccaca taaaaactgg tggaacatta ttaaatcaaa
    12721 gatttgcacc atcagtagtt caaggagaaa aagggttaga taatatggct aatctagtaa
    12781 gagcatactt taatatggat ggtcatcata tacaatttaa tgtatttgat aaaaatgtac
    12841 tgttagaggc tcaaaagaat cctcaggatt ataaagactt aatagttaga gttgcaggat
    12901 atagtgacca tttcaataat ttaagtagaa cactacaaga tgagataata ggaagaactg
    12961 agcaaacatt ctaggtttgc ttataaataa aacttcttac tatataaaat tgtaactgat
    13021 ataataaagt ataaatggtt tgattccatt tttaaaattt tagattaatt atgagctaac
    13081 tttctaaata ggaagttagc tcgttaagtt taatgaaaaa atcgttaaat ttatatttag
    13141 taaatcatac tcatattagt aagtaataca tacaaaagaa taatttatta tagttataat
    13201 aaggttatat tttataagaa taagataatt ttaagcagtt aaaataaagt caatttagct
    13261 actttaaaat aaaactacta gaaaaaagga gatttgatat gaaaacttta ggatttattg
    13321 gttcagggaa tatgggaagt gcaatgatag gtggtatagt acagtcagga ttagtaaagg
    13381 cagaagatat aactgtatca gatttaagcc aagttgcctt agataaagta aaagaagaat
    13441 ttggagtaaa tactacaact gattcaaatg tagctgttac aaattctgat gtggtaatag
    13501 tagcacttaa acctaatata tgtgaaaagg ctttaactcc acttaaagga ttaatagatg
    13561 taaataaaat aatagtatct atagcagcag gtaaaactat agaaagtctt gaaagtttta
    13621 taggttcaga taaaaaaata gtaagagtaa tgcctaatac tccagccctt gttggggagg
    13681 gaatgtcagc tctttgtaaa aatagtaatg taacagatga agagttaaat atgattaagg
    13741 ctctgtttga atcttttggt gaggcagaaa tagtatcaga atatttaatg gatacagtta
    13801 caggtattag tggttcttct cctgcatatg tatttatgtt tatagaagct atggcagatg
    13861 cagcagtatt agctggaatg ccaagacaac aagcttataa atttgcgtct caagcagtaa
    13921 tggggtcagc taagatggtt cttgaaactg gaaaacatcc aggtgaatta aaagatatgg
    13981 tttgttctcc agcaggaact actatagaag ctgttaaggt attagaagaa gaaggcttta
    14041 gagcagcagt tataaaatct attgttgctt gtatagataa gtctaaagat atgagcaaat
    14101 aacagatgta attaagttta ctatatggtt tcttgaattg cttttttagt gcttattta
//
LOCUS       DAEMAE010000089        13983 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEMAE010000089 DAEMAE010000000
VERSION     DAEMAE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.091"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            149..2026
                     /locus_tag="KPH93_002077"
     CDS             149..2026
                     /locus_tag="KPH93_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1688897.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNEVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AVGGAIMGMGGAYGNAFANQGILTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKIETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLRLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKNAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLVG
                     N"
     gene            2338..3744
                     /locus_tag="KPH93_002078"
     CDS             2338..3744
                     /locus_tag="KPH93_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG1688898.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFITLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3772..4620
                     /locus_tag="KPH93_002079"
     CDS             3772..4620
                     /locus_tag="KPH93_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1688899.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSRERIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5282..5974
                     /locus_tag="KPH93_002080"
     CDS             5282..5974
                     /locus_tag="KPH93_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1688900.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEIAEFTARVLSLIRRYTRFNPKSEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            5971..6906
                     /locus_tag="KPH93_002081"
     CDS             5971..6906
                     /locus_tag="KPH93_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1688901.1"
                     /translation="MKMDEQTIILFLFLLSIGLLLLSIFAFFKLGHIHKRLKDIEEML
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLDFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLTHSKACRIKITISEDSKMVLFRVEDNGVGIAKENLQHIFERLYKCDKGRSKKGSGL
                     GLSIVSQMVERMGGQISVESELGKYTVFTVSLPLI"
     gene            7055..7978
                     /locus_tag="KPH93_002082"
     CDS             7055..7978
                     /locus_tag="KPH93_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688902.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALEVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEISLLADDIGIIDQGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLEIQLNLKNFKVADNHNIMLYDTDVSVAEINRAF
                     IQNNIDVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            7962..8732
                     /locus_tag="KPH93_002083"
     CDS             7962..8732
                     /locus_tag="KPH93_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688903.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTALPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFKPNNLLLSILPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8742..9524
                     /locus_tag="KPH93_002084"
     CDS             8742..9524
                     /locus_tag="KPH93_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1688904.1"
                     /translation="MLDIITTEFQKIKRYNILWVGVVAVLFSALLAVFQIVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTEQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGTKHKKYSHK"
     gene            9901..10437
                     /locus_tag="KPH93_002085"
     CDS             9901..10437
                     /locus_tag="KPH93_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG1688905.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10468..10857
                     /locus_tag="KPH93_002086"
     CDS             10468..10857
                     /locus_tag="KPH93_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG1688906.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGNPDRFDGRIDNHYHIICEVCGEIYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICKNCKKCSQVG"
     gene            10894..11280
                     /locus_tag="KPH93_002087"
     CDS             10894..11280
                     /locus_tag="KPH93_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG1688907.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11407..12849
                     /locus_tag="KPH93_002088"
     CDS             11407..12849
                     /locus_tag="KPH93_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG1688908.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKVAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDMDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13147..13962
                     /locus_tag="KPH93_002089"
     CDS             13147..13962
                     /locus_tag="KPH93_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1688909.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCLIPKK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGMTISI"
BASE COUNT         5041 a         1712 c         2670 g         4560 t
ORIGIN      
        1 acgtgttact ggtaatgcag gcaagatcga atcaattttt atagattatc tatagagtac
       61 tatgggtata atctatttat ttggttcgat ttttttgttt aattggccat aaaacaaaaa
      121 gcctatagtt gaaaatggag gattttttat ggattataaa aatatagctc aagaaattct
      181 actaaatgta ggaggaaaag agaatgtaaa tgaagtgact cattgtatga caagacttag
      241 atttaaagtc aaaagcgctt ctaaagtgaa taaggataaa ttatctaaaa cagaaggagt
      301 aataactgtt gttgagagta tgggtcaaat acaagttgta attggaaata aagtaaaaaa
      361 agtatatgat gaggttataa aaattgtacc tcaatcaaat gaagtaggta aaaagaatga
      421 aagtcaagaa aaacaaggta taattaattc tatattatca gcagtagctg gtatatttac
      481 acctacaata cctgctatag caggggttgg tatgattaaa ggtattttat cagttcttgc
      541 tatgtattat atgaataaaa atggagtaga tataaaagaa acacaaagct atattatctt
      601 aaatgccatg gccgattcta tattttattt tatgcctatt atactaggat atactgcggc
      661 taaagtattt aatgcaaata aaataatatc tatggtatta ggagctacac tttgttatcc
      721 aacttttact gctttgatgg caggggaaga atctgttaga tttttaggat tggcagtaac
      781 aaaagctacg tatacatcat ctgttattcc aataattatt gctatatggg ctctttcata
      841 cgttgaaaaa gtattggaaa agtatatacc tgaggtaatt aaaataataa tggttccaac
      901 tttatcattg gttgtaatgt tacctgcaac attgttttta tttggaccaa tcggaattta
      961 tataggaaat gtcataaact tttcttataa gtatatttat gaattaagtc cagcattgtg
     1021 tggagcattt gtaggtgggt tatggtgtgt gttggttata tttggtgctc atagagcact
     1081 attaccaata ggtataagtg atgttgctca aacaggaaga cagaatttat tggcatttgc
     1141 aggagctgca aacttttcac aagcaggggc tgctctagga gtattcttta aaaccaaaaa
     1201 tcaaggacta aaaacaattt ctatgtctgc aactataaca gctttatttg gtataactga
     1261 acctgcaata tacggtgcca atcttagatt aaaaaaacct atggtatgtg ccgtaatatg
     1321 tggtgcagtt ggaggtgcca taatgggaat gggtggagct tatggtaatg catttgcaaa
     1381 ccaaggtatt ttaactatac ccgtatatgc tgaagcaggt gcattaggtt tcttaagtta
     1441 tctaggtggt tgtgctatag cattttttgg ttcagctatt tcaacttatc tagttggatt
     1501 tgaagatttg gaagagtctt ctaataagga aaattcatct ataaaaatag aaacgaagga
     1561 tggtgttatt gatataactt cacctgtaga gggagaatgt atagaattat cagaagtaaa
     1621 ggatgacgta tttgcttcaa aagctatggg agaaggtata gctgtgttac ctacaaaagg
     1681 agtaataaca gctcctactg attgtgaagt agcttcttta tttccaacat tacatgcaat
     1741 aggattaaga ttagataatg gagcagaaat gttaatacat gttggaatca atacagtcga
     1801 gttaaatggt aaatacttta caaaacatgt taatcaaggg gatttagtga aaaaaggtga
     1861 taaacttata tcctttgata tagataaaat aaaaaatgct ggttatgatg taactacgcc
     1921 agttatagta aacaatacat ttgattttgg gcaagtagtt tcttgtaaat ctagttatgt
     1981 ttcaaccaat gataatataa tttcattagt attagttggt aattaagtta atttgactag
     2041 agataagtaa attgatttaa aaggaaataa gtaaatcaac aattaaatac atctaaaagt
     2101 aagtttattg caatgaattg ttaaagaaaa taattaatat ggagtaaagc tattgcaatg
     2161 aagtaacaaa gaaaataatt aatatagagt aagtttattg taatgaattg ttaaagaaaa
     2221 taattaatat agggtaaatc tatctcaatg aaataacaaa gaaaagagtt aatatagagt
     2281 gaacttattg caatgaatca acaaagaaaa taatcaatat aaaaaggaga aatatttatg
     2341 aatacaggat ttccaaaaga ctttttatgg ggagcatcat cttctgcatt tcaagtggaa
     2401 ggagcttggg ataaagacaa taaaggaaaa accgtagccg actataattc ttttaaaaag
     2461 tctcatttac aggcagatac taaagtcgca tcagactttt accataatta tgaagaagat
     2521 attgaattaa tgaaagaatt agggatgaaa acatatcgtt tttcaatttc atgggcaagg
     2581 ataataccag atggtgaagg agagataaac cagaaaggtt tagactttta taataagata
     2641 atagacaaac taatagagtg tgatattgag ccattcataa ctttatacca ttttgacctt
     2701 ccatttaaat tagtagagaa atataatggt tgggaaagta gagaaactgt ctatgctttt
     2761 gaaagatttg ctaaaatatg ctttaagcat tttggagata gggtaaagta ttggcaacct
     2821 cataatgaac agaatttaat tgttagagtg gaggagagaa ttaatatata tgatgaaaca
     2881 gattcatgga agatagataa aatgcgtgct cagatggatt ataatctttg cttagcacat
     2941 gcattagcag tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg agcagcagta
     3001 tcatcatctg taacatatcc tcttacttct aagccagaag atatatatgc agctagaatg
     3061 aatgataatt ttaaagtgta ctatatgctt gatatgcatc attatggaga atatccaggg
     3121 tattatatga agtatcttga aaaaagaaat atagttcctc acatggaaga tggggataaa
     3181 gagatactta aaaaagctaa aatggatttt atagctgtaa attattatag gacaaattgt
     3241 gcagaagctt tacctgaaga ttcacaacat ccatttggat taagagaagg aactgttgat
     3301 tttagtatgt atggactatt taaaatgtca atgaatccta acttagaggc ctctgagtat
     3361 ggagcagcaa tagacccatc aggtcttaga gttgctttaa atgaatattg gcaaagatat
     3421 cacttacctg ttataataac agaaaatggt ctaggagcta aagatatatt agaagatgga
     3481 aaaattcatg atgactatcg tatagactat ttaagaagtc atataaatgc atgtaaacta
     3541 gctattgaag atggtgtaga gatgattgga tattgtccat ggtcatttac agacctttta
     3601 agttcttctc aaggttttaa taagcgttat ggacttgttt atataaatag aactgaccat
     3661 gaagttttag atttaaaaag aatcaagaaa gatagttttt actggtataa agaagttata
     3721 gagaacaatg gaatagtaaa ataaacataa atatatattg agggttttag tatgtacatt
     3781 acaaaggtat taaataattc gttgctactt gcaaaggatg ataatgatga agaaataata
     3841 ttgatgggga aaggcattgg ccataattat aaggccgggt ctgagttaaa taaaaaagat
     3901 atagaaaaga tttttgttct tcatgatgaa aatataaaaa agagtattat acaattggca
     3961 acagaaatag atgcagaata ttttggtata gctcaaatga taattgcata tggaatagaa
     4021 aaatataact taaaacttat gaaccatata tatttggcct taactgacca cattgccttt
     4081 gcagtgagac gttttaaaga aggtataatg attgaaaatc actatttgtt tgaaatcaag
     4141 gagtttaatc caaaagagta tgatatagga aaatatgcaa ttactgtttt taaggaggtt
     4201 cttggtttag acctaccaga agaagaaatt ggtaatatag caggccattt tataaatgca
     4261 caacaagata atccttatag tgatagaaat aagagaagtg ctaaaattgt aaatgctatt
     4321 ttgcaaatag ttcattatca cttttctatt gtatataata aagagtcatt ttattatagg
     4381 agatttgtaa tgcatctaaa ggccttttca caaagatttc tggctaatga accatcaaga
     4441 gaaagaatag attttatata tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt
     4501 gtgaaaaagg taggagctta tatgtataag gaatactcca gagaattacc aagacaagag
     4561 gagttatatt tgatgattca tattcataag attttaggag aattatgcga aaatagttag
     4621 aatatcttaa tatgctagta atattataaa aaattaaaaa ttaactacac tttaaatatg
     4681 aataattgca ctacttgagg gagttgtctt aaaattgatt tttttaatta aatttgaagc
     4741 tcaactcctt ttttaaacct ttatatcatt acgactttaa tgtttatacg cctgtacttc
     4801 agatatatat ctacttttta catttctcaa catgcaaagc tatcattttt tgcagcatgt
     4861 taattgaaat aaacctttga gttaacgcta aatttattct ggacttacag gaatagttcc
     4921 aaagatactt taaaatattt tctggaatga ttgcttgttt catatatatt ttattgtgat
     4981 gttttgattt aataaattct tcaactctgt atatagtaaa tgtattatca tgtttgtttt
     5041 attcaagcac acttataata cgcttgattt tttcacaatg atgaaatata ctaatgggtg
     5101 ttgtagatat aagaatcaca ttacacttga aaaagtaagt gagaaatgag gtataactcc
     5161 tcatatgata aactactatt tcattgtgga acatattcta agtgatgtca agatactaca
     5221 atatgactaa attcctaatg ataaaaaagt ctacgaatga gtgtagaaag gggggagtaa
     5281 gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt taaaaaagag
     5341 tatctcaatt gaacagattg aagcaaacta ttgccattct gggagagatg gactggttga
     5401 actttcaaaa caagattatc agttagtagt tctagacgtg atgatgccag gtatggatgg
     5461 ttttgaaact atggaacaaa tacgaaaata tagcagtatt cccatcttaa tgctgacatc
     5521 gaaaagcgat aattacagca aagtgcatgg attgcgagct ggtgcagatg actatctaac
     5581 caaaccattt gaaattgcag aatttactgc tcgtgtactg tctttaatcc gacgttacac
     5641 ccgttttaat ccaaagagtg aggagccaca gcaactttct ttcaaaggtt tgttgattga
     5701 ccttgaccag catacagtga ctaccaatgg cgaacctatg ttgcttccac ccaaggaatt
     5761 tggtgtgctt ctttactgtg caaggcatca gggtaagatt ttgacaaaac aacagattta
     5821 tgaggccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg ctattatcag
     5881 tcgcctaaga aaaaaaattg aactagaccc atcctctcct atctatttgc aaacgataaa
     5941 aggaatcggg tatcaattca acacggaggt atgaaaatgg atgaacaaac gataattctt
     6001 tttttgttct tgctgtcgat tggtctgctt ctcctttcca tattcgcctt tttcaagctt
     6061 gggcatattc acaaacgtct aaaagatata gaagaaatgc ttgctgatgt acaaattgga
     6121 aacgaaaatc gtaaaatact aattaagcca tgtgatgtta tggctccatt ggtttatcag
     6181 cttaatgaga ttgtttacga ttatgaaaat aagttacttt ctctgaaaaa gtcggataaa
     6241 gctagcaagc aacttatgac aagtttatct catgatgtcc gtacaccatt aacaactctg
     6301 attggttatc tagatgctgt acacagtggt atagttatcg gacaagaacg ggaggaatac
     6361 cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt atatcgatgt tctttttgac
     6421 tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtgga aattgcagag
     6481 ttgtcacgca atattcttaa agattggatt cctatcttcc atgaaaagaa attggatttt
     6541 gaaattgata ttccagaaaa tcgactaatg gtaaaccttg atccagatgg atattctcgt
     6601 gtagtcaata atctggttca aaacgttctt actcacagta aggcttgtcg aatcaagatt
     6661 actataagtg aggattctaa gatggtatta tttcgtgttg aggataatgg tgttgggatt
     6721 gccaaagaaa atttacaaca tatttttgag cgactgtaca agtgtgataa aggacgttca
     6781 aaaaaaggca gtggtcttgg cctatctatt gtcagtcaga tggttgagcg aatgggtggt
     6841 cagatttctg tagaaagtga acttggaaag tatactgtat ttacagtgag cttaccatta
     6901 atataaagat tgtttttccc ccatgtgaca tatataaaca gggggatttt ttataagcta
     6961 taaagttgca aggttaatac aaggttgttg caaggttaat gtaagattga ggtggtatga
     7021 taactttaat aaacaaagga ggattttact atttatgaat aatttaatta ttgaaacaaa
     7081 aaatctcact aaacaatatg gtgctcaaaa gagcgtttct aatttgaata ttcatgttca
     7141 gaaaggtcgt atttatggat tgctgggaag aaatggtgcg ggtaagacga caactatgaa
     7201 aatgttattg ggtttaacaa aaccaactac tggagaaatt aaaatatttg gtcaggacat
     7261 tcataataac gagaagaagc ttcttcctag aattggatgt ttgattgaat caccaggttt
     7321 ttatcctaat ctgacaggaa cagaaaatct caaaatcttt gcaaggctta gaggaatacc
     7381 aaaaaaaaat gcagtagaat cagcactgga ggtagtcggt ttgccaaaca atgacaaaaa
     7441 gttattttct caatactctc ttggcatgaa acagaggctg gcgattgctc ttgccattat
     7501 gcatgaccca caattgttga ttttggacga gcctataaat ggacttgatc ctattggtat
     7561 tgcagaagta aggaagttta ttcgtgatct ctgcacagaa cgaggtaaaa ctattttgat
     7621 ttccagtcac attctttctg aaatatcatt gttggcagat gacatcggta ttattgacca
     7681 gggcattttg ttggaggaag aaagccttgc acaactggaa gaaaaaaaca gtaaatacat
     7741 acattttgct gtatctgatg cagcacaggc agcaaggctt ctggaaatcc aacttaacct
     7801 gaaaaatttc aaagtagcag acaatcacaa tattatgctt tatgatacag atgtttctgt
     7861 tgcagaaata aatcgtgctt tcattcaaaa caatattgat gtatcagaat ctcatttgtg
     7921 tgaagatact cttgaagatt acttcaagaa agtaacaggg ggtgaaggaa ttgcttgata
     7981 taatttcatg cgaattttca aaactcaagc ggttaaaatt tatactgatt tctattttag
     8041 gtgcttgctt atttccaatt cccgcaacaa ttttgattgc aaaggacaac ttgccctttg
     8101 agcaattgtt taaattagtt gtcaattttg gatatttttt gctattacca attgtattga
     8161 gtattgttgc tagccaacta tttttcatag aaagagataa cgatgtatta aaaaatcttg
     8221 caacagtgcc agtgccaaaa ggaaaactgg ctctggcaaa attagctgtg ttgctgttta
     8281 tatcattgtt ctactccgtt gcagggttag gagctactat cattggaggt ttcattgttg
     8341 gaatagtaga gggggttgct gtaaaattag gaatgagcat tgcttttgga attatgtttt
     8401 ttgttactgc acttcctgtt gtggtactca ttgtatattt caatcgcagc tatatttttt
     8461 ctattatact ttcttttgta tatgctattt tcaattttag tatatcctta aatattatca
     8521 attttaaacc aaataaccta ctattaagca tattacctgc tcctgttata atgcgttggt
     8581 ggatggcatt ttggggagat cccactggcg aatacacggc acttagacag ccatatttgc
     8641 tgtcaactcc tgcttgtgta ggtattttat gtttgataac cattatagca gtcctgctga
     8701 tttgtatgat atacaaaaag caggagaatt aggaggtatc tatgcttgat ataattacta
     8761 cagaatttca aaaaataaaa agatataata ttctatgggt tggtgttgtt gctgttctgt
     8821 tttcagcgct attggcggtt ttccagattg taagttcaca cggtagtgac cctctaacgt
     8881 atgagagctt tgcaaacgga gttatctgga ataatttcag tcttagtttt ccatttggga
     8941 ttacacttat tggaggatat cttattaacc gtgaatatac agaacaaact ttaaaaaaca
     9001 tcctaactgt tcctatttca ttacgcaaat tgctagtggg aaaattaatt gcggttggag
     9061 gaattactgt attgttaagt cttttcagct tcttatgcac atttatttta gggttgctat
     9121 tttgcaatat caatatatct gttacactcg ttgtaaaatc cttagtccaa attttgtctg
     9181 ttaatctgta ttgttatgtt gctgttttgc cgattattgc ttattttggt cgtaaacaaa
     9241 atgcatttct gacaggtgta gggattgcat ttgtatacgg tttttgtggt gtgtttgttg
     9301 ctggaagaaa tctgacagac ttttatccaa tcacagcagg tcttggtcta gttggataca
     9361 acaatggtgc tggtactgtt tatcagccac ttatcggtgc tatgactttg attgtaattc
     9421 taatcttgac aactattcta cttgtattta cacctaatta cgataaagtt atgactatat
     9481 ctaagaaaaa agggacaaag cataaaaagt attctcacaa ataatattaa tattgttttt
     9541 attatttaag ttgccagaca gtgaaaagaa aaaatctgtc gtacagtggt tatatgttca
     9601 tgtattcaag aagctacagt tttgattttt catagatgta gcttcttttt atatttatta
     9661 aattacgact atcacaatat ttacaatagg tatagtaaca agagagaata ctgtcatatg
     9721 tgttttatat attacattaa gtatattcta aaatatatgc tttaaataat atatttgaaa
     9781 ataggaatat ttaaataaaa aaaataatat tatatatagt tgacaaaaac tggaaaatga
     9841 tatactatat aaatatagaa atagtaatca ttactattat taaaaaggga ggaattaatt
     9901 atgaacttaa aaggaactaa aacagaaaag aatttaatgg caggatttgc aggagagtca
     9961 gaagcaagaa ataaatatac atactacgct tcaaaagcta gaaaagaagg ttataatcaa
    10021 atagctgcta tatttgaaga aactgctaac aatgaaaagg aacatgctaa attatggttt
    10081 aaacttttac atgatggtat gccttcaaca gaggaaaatt taaaggatgc agcagcaggt
    10141 gaaaactatg aatggactga tatgtatgca aaatttgcta aagaagctag agaagaagga
    10201 tttgacaaga tagcttattt atttgaagct gttggaaaaa tagagaaaga gcatgaagaa
    10261 agatacttaa agcttttaga aaacttaaat gaaggaaaaa tatttaaaag agatgaagaa
    10321 gttgtttggc aatgccaaaa ctgtggtcat gtgtatgtag gtactgaagc accagaaaaa
    10381 tgcccagtat gtgaccatcc aaaagcatac tttaatataa aagctgaaaa ctattaaaaa
    10441 ttattataat taaaaggaat gttaactatg aaattttcta aacaacgaga actgattttg
    10501 aatgaaatat taaataatcc agttcatcct actgctgatt acctatatga aaacttaaaa
    10561 aaagataatc caaatttaag cttaggaact gtgtatagaa atttagctca gctaacggaa
    10621 catggcttta taagaaaggt tagtattcca gggaatccag atagatttga tggtagaata
    10681 gataatcact atcatatcat atgtgaagta tgtggtgaga tatatgattt agaatctgaa
    10741 gttcttaata atttacaaga attaatatct gaggagacag acataaaaat aacatcttat
    10801 aacataagtt ttaaagggat ttgtaagaat tgtaaaaagt gtagccaagt aggttagttt
    10861 tcaaattcta aaaataatta atgaggtgag tttatgtgta gtgaacaaaa attttttata
    10921 tgtgagactt gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc tattttctgt
    10981 tgtggaaaac ctatgaagga acttgttcct aatacaacag atgcagcagt tgaaaaacat
    11041 gtacctgtga ttgaagtgga tggaaataat gttactgtta aagtaagtag cacaactcat
    11101 ccaatgacta aagaacatca tatagcatgg gtgtatctta tgactgaaca aggtggtcaa
    11161 cgcaaatgtc tagcagtaga tggagaacct gtagttaaat ttgctttgaa tgatgatgat
    11221 aaagtgattt ctgcctatgc ttactgtaat ttacatggat tatggaaggc tgaactgtaa
    11281 tatttaaata aattgaatca aaaaggaggc tataaatgta ttttcataca aatagcctcc
    11341 tataaattta tataaattca agttttaatt ttgtttaatg tcaatataat ataaggagaa
    11401 aataatatgt caatttataa atgtagtgtt tgtggatata tttatgatga atctaagaat
    11461 gataaaacat gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac aaaaggtcgt
    11521 agttattttg gaaagataag tactgtttac tatgaagaag atgaaaaggt agcagaagac
    11581 atagttgaag atgaatctaa attaaatact gaaaaagagg gagatttaaa ttacttaagt
    11641 acatatttaa gaagagatga tgaagttgag aaacatatgg atataattca cgaaatggct
    11701 gttacaggaa aatctataat agaacctatg agaactaaac taccagtgat ttcttgggat
    11761 gatattttaa ttatgggtgc tcaattaaat ccattacctt taaatgagca tgatgaagta
    11821 aatacaacga caataatagg aaaaaaggcg aagaaaccta tgattataga aaatccagtt
    11881 tatatttccc acatgtcatt tggagctttg tctaaagaac taaaaatagc tttagcaaaa
    11941 ggtgcagccc aaaataagac agctatgtgt agtggtgaag gaggtatatt accagaagag
    12001 aaggaagctt catacaagta tatttttgag tatgtaccaa ataaatatag tgtaactgaa
    12061 gaaaatctta aaaattctga tgctatagag ataaaaattg gtcaaggaac aaaacctggt
    12121 atgggaggtc atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt aagaaataag
    12181 cctgtaggtc aagatgtaat aagtccatct tgttttgagg aaatacaatc caaagaagat
    12241 ttaaagaaac ttgtggatga acttagagaa gtttcagaag ggcgtccaat aggtgttaaa
    12301 atttcagcag gtcatattga aaaagatatg gaatttatag cttatgctaa acctgatttt
    12361 gtaactattg atggtcgtgg tggagctact ggagcaagtc caaagttgct taaagatgca
    12421 acttcaattc caacaatatt tgctctttat cgtgctagaa aatacataga tacacatgga
    12481 ttagatatgg atttggttat aacaggtggg cttcgtatat ctactgattt tgctaaggca
    12541 attgctatgg gggctgatgc agttgctatt gcaagttctg ctcttatggc agcggcgtgt
    12601 caacaatatc gtatatgtgg ttctggtaag tgtcctgtag gagtagctac tcaggatgag
    12661 gaattgagaa aaagattaca tatagaaaat agtgctaata gagtggcaaa ctttttaaat
    12721 gtaagtttag aagaacttaa gacctttgct agaatttctg gtcataaaga tatacatgat
    12781 ttaagtgttg atgatttata tactgttaac tctgaaatat caaattatac aaacatacag
    12841 catgtataga ttgaagtttg aaactattag attgtagata gttataaaag taaatgataa
    12901 atatatttta taaaaaggag tgtcttaaaa tgaacttttt agtttataag tcactctttt
    12961 ttgtatgaag caaaatatat ttatcatttt aacaatgaaa aagaatctta tctctttact
    13021 ttgagataga ctccttctat ttatgaaaat taaaagatat taaattgtaa aatattctgt
    13081 gatgtcgtac tattaaacta aagatgctgt attttaaagt aaataaaaat ttaaggagtg
    13141 attattatgg aaagattgaa gatacaagtt ctagttgaca ataatacata tattgatagg
    13201 tactttgttg gagaaccagc agtaagctat tacattgaga ttgatggaaa taggattttg
    13261 tttgattctg gatattcaga tgtattcata tcaaacgctg aaaaattaaa tatagattta
    13321 ggaaatttaa cacatgttgt tttttctcat ggtcataatg accatacaag aggtatacag
    13381 tttttagaaa atagatatga tttgtctaca gttgaactta tttcacatcc taactgtctt
    13441 atacctaaaa aacatggaga gaaaagcata ggagctccat tttctgctga ggaaattaaa
    13501 aatattttta tgtataatcc taaggataag ccatttaatc taagtaagaa ttgtgtgttt
    13561 ttaggtgaaa taccttctat aaataatttt gaaaaaagag ctaaaattgg taaatgtaaa
    13621 ataggagatt tgtgggaaga tgattatgtt ttagatgatt ctgctatagt ttgtaaaact
    13681 gataaaggta tttttattgt tacaggatgt tctcatagtg gaatttgtaa tatagtagag
    13741 tatgctaaaa aggtatgtgg agatgataga gttattggta tattgggtgg ttttcatctt
    13801 tttgaattga ataataggtt agatagcaca atacaatatt tggataaaga agaaataaga
    13861 atgttgtacc cttgtcactg tgtttctttt aaagtaaaag ctaagatgaa tgaaatattg
    13921 aatataaatg aagttggagt agggatgact ataagtatat agtatttgaa agtatataag
    13981 att
//
LOCUS       DAEMAE010000090        13868 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEMAE010000090 DAEMAE010000000
VERSION     DAEMAE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.092"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            300..1004
                     /locus_tag="KPH93_002090"
     CDS             300..1004
                     /locus_tag="KPH93_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1688910.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKVWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            1006..2493
                     /locus_tag="KPH93_002091"
     CDS             1006..2493
                     /locus_tag="KPH93_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1688911.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTETVEHMDSNFRPIKPPQYDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCL
                     AGILAIFTAVILSEKIAKPIKELCEFAKKIGQGDFKRNYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFQLVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2698..4311
                     /locus_tag="KPH93_002092"
     CDS             2698..4311
                     /locus_tag="KPH93_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1688912.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKIVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMFENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQVWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFESFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKFEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4531..5508)
                     /locus_tag="KPH93_002093"
     CDS             complement(4531..5508)
                     /locus_tag="KPH93_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1688913.1"
                     /translation="MIQSEISDRSNYKKINTLEVVIFFVVTYGISLLFGIPLYFKEYM
                     NPQIMVTFMTILPASGVSIAKIYKHKKSDEHIILHCVIIADFLLSLFLVTLKAFGAID
                     DETVILGMGILVLFTGIITICYACLEGKELYPFKNVNKSIICIFIFIIIINLSKCIML
                     GWKDINFINLIMNIFLIFPYIANQSILFFGEEYGWRGFLQEKMQTKFGKRMGVILLGI
                     IWELWHIPLWFTIYHVDGLGILLRLFSVISLSIFFGYVYMKTKNVWSCVVIHLINNTV
                     LLDTNLNNAFLNFNTSSLNSSVILGCLIFSVLLSLFIFSKEYKKEQLIY"
     gene            complement(5525..6355)
                     /locus_tag="KPH93_002094"
     CDS             complement(5525..6355)
                     /locus_tag="KPH93_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1688914.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKTYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTIILKEHEEEYLVKTPIIYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKAKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLCMGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            6428..8155
                     /locus_tag="KPH93_002095"
     CDS             6428..8155
                     /locus_tag="KPH93_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688915.1"
                     /translation="MKKLIHFLKPYRVLIVIVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKVMTVIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQVKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGREDVVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            8193..10055
                     /locus_tag="KPH93_002096"
     CDS             8193..10055
                     /locus_tag="KPH93_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1688916.1"
                     /translation="MSEKRTDKQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQFIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASSRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKLDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGRHKELIEKKGFYEELYNSQFSSEYDEDVV"
     gene            10388..11077
                     /locus_tag="KPH93_002097"
     CDS             10388..11077
                     /locus_tag="KPH93_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG1688917.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPTMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVATREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSASL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            11182..11868
                     /locus_tag="KPH93_002098"
     CDS             11182..11868
                     /locus_tag="KPH93_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG1688918.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            11885..13783
                     /locus_tag="KPH93_002099"
     CDS             11885..13783
                     /locus_tag="KPH93_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG1688919.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKGVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSNSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKVTKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKEFNNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSIVKT
                     NTDNETKNKTTNDSENKNNTNKDKSGNDNNQKLKGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDENSSGIKGTEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQGNPGGFGNRGGGSMSKTTIYSKLILGGASMIIMSIMLVGVSRVK
                     RRRFIKSK"
BASE COUNT         5378 a         1470 c         2349 g         4671 t
ORIGIN      
        1 ttatcaaaaa tatgcaaagc actacacctt caaatatcac ctaaaggaat taaaaggtaa
       61 ttaaagtgaa gtagttcaag atatttctaa aatgagttta ataaattcta taataattta
      121 ttttttgcct taaaattgcc ttaaaatcca aacaatttta aaactaatgt actataaaaa
      181 gctggtataa tttaaatata gtaaaaaata agatttatct tatctattgg gatttaatta
      241 tttaatcaat atatttatac tatttaattt aaataaaaag tctattaagg agtgagctta
      301 tgagtaagtt aatttatata gcggatgatg aagataatat aagaaatttg gtaaagacat
      361 tcttgaaaaa tgaaggtcat gatgttatgg attttaaaac gggtgatgaa cttttagaac
      421 aattcaatat aaaggagtgt gacttagtaa tactagatat aatgatgcct ggttcaagtg
      481 gttttgaagt atgtacaaaa cttcgtgaga aaagtacagt cccaataatc atgcttactg
      541 caagagatac agatatagat tatattacag ggataacctt aggtagtgat gattatttta
      601 caaaaccatt tagcccaatg tctttagtta tgagagtaaa atctatattt agaaggatag
      661 agtttgaaaa gaaacaaaat tatgataaat atagtaactc gatagatatg gaattaaaat
      721 ttggggatgt aataataaat aaaaagaata aaatcgttac aagtaaaaat gtaaatatag
      781 accttacacc aaatgaatat aacctattga cttatttgtt tgaaaatata gatagagcag
      841 tttccagaga tgaattatta aacaaagtat ggggatatga tatagaagtt gagacaaggg
      901 ctgctgatga tacagtgaag agattacgta aaaaaatttt agatacaaat attttaatag
      961 aaactgtatg ggggtttgga tttaggttga aggagaaaag ttaagatgaa gaatggaaat
     1021 gacaagaata gaattggaaa gattaaatcc aaatttaaat taaagttctc aaagaaaaat
     1081 ataagcataa aaactaggat tttgttgagc atactttgtt tgataattat agtatttatg
     1141 ataatattag tatcttttaa tgtatttctt gatacatata taaaaacaac tgcgaatgaa
     1201 gaattgacaa agtctacaga aacagtagaa catatggata gtaattttag acctattaag
     1261 ccaccacaat atgatggaaa gcttcctccc aaagtattat ctaattttat gagaaatgta
     1321 caagataaag ttaggatggc agaaacacaa tcagatgctg atgctatggt agttgattct
     1381 aagtataacc tgatatttcc aacaagagag gatgatttct taaaaaatgt tgatgagatg
     1441 gagctgatta gggagtgtat tcaaaatgaa aaattagatt taaattcaga tgaaaataca
     1501 cggatatcta ccagaaataa ggattattat atttcaactg taaaaattac tagtatatat
     1561 ggtgaacaag acaagtatct tatacttttt atagacatat caaaaactct aaatctagca
     1621 caaaagataa atattgtatt aatatctgta atgtgtcttg ctggaatatt agcaatattt
     1681 acagctgtga ttttatctga gaaaattgct aaaccgataa aagagttgtg tgagtttgca
     1741 aaaaaaattg gtcaaggaga tttcaaaaga aactactttg acttttcaga taaagaatta
     1801 gtagagctct caactgttat gaataagagt gctgagtatc tggataagta tgataatgaa
     1861 caaaaaatat tttttcaaaa tgcatctcat gaattaagga caccactcat gtctattaaa
     1921 ggttatgcag aagctataaa atataatgtt atagatagta aacatgcaag tgatataatt
     1981 cttgaagaaa gtgataggtt aagtgatatg gttgaagatt tattatacat ttctaaaata
     2041 gataatataa ctaaagattt tcaacttgta gaatgtgatt taagagaagt actttcaaat
     2101 tgtggagcta ggcaaaatgt aagagctata aataagggga taaaatttat ttatgaattt
     2161 gatgaagaaa tggttttatt tgaatgtgat gaaaaaaaca tttctaaagc atttatgaat
     2221 cttatagaaa atgctttgag atacgctaag agtgaaataa aaatagtgtg taagtataat
     2281 caaaaaaata tagttgtgat aatagaagat gatggaattg gaatcaagaa agaagattta
     2341 cctcatgttt ttgaaagatt ctataaggga gtagggggaa atcatggaat tggattatca
     2401 atagttaaat ctattgtaaa taagcatggt ggacgtatat acgtagaaaa tggcaaaaaa
     2461 ggagctaaat ttactatatt atttaaattg taaaaaaatt agagaaaata tagagatgtt
     2521 tgaagttgtt ttttatatat atctgttgta tactgttgaa aaaagaacaa tattaagctg
     2581 acatatatta cgatagttga catttaaagt aaatgcttaa atatagatac gaaaggaaaa
     2641 gtaaaatctg aagtgtattt aggtttaagt aagcttctta ttttataaaa aatatgtatg
     2701 aaagaacagc tgatttttaa tattacaaat ttagtaacgg ttgtatttga agcttttgtc
     2761 atccatatgt ttttatcaga ctttttaggc cagaaagaag ggtatataaa aattgtaaga
     2821 tatgctaaac tgggttttat aatatgttta ggattctgta atttaataac tttaaaccct
     2881 aaaatcacaa tgcctttaat tttcatcctt attttttcaa catcattttt atataaagga
     2941 aatttaaaga cacgattgtt tactactgtt ttattaagta tattctttat actgtcagaa
     3001 attgtagtta caagtatttt tgttttattt gtaaaagaag gttttgaaat aatgtttgaa
     3061 aataatagta taagagtatt ggcgacaatt ttatctaaaa ttgtcttttt gttgacttgt
     3121 aaaattattt gtctatttaa aaaagatgta catttagata tgcccataaa gtattggctc
     3181 cctttatttt taatacctat atttagttta tttctgtctg tttctatctt tgatgtaagt
     3241 aaatttttta gtcttgaaag tttaaaattt ctaagcttaa tatctagtgt tggtatttta
     3301 tatattaatt ttattgtctt ttatttattt aaatttatta tagataaaac taaactatct
     3361 atgaaatatg aaatacttga aaaagaaata attcataagg aagaattaag acttagtaat
     3421 gagtgttata aaattattgt tgaacagacc gattctgtcg tttttgaatg gaatatcaaa
     3481 gagaataaat cgtttgtatc gcaggtttgg actgaaaaat ttggatataa caatgcatgt
     3541 aagaatattt ttaaagaaat taaagataaa gacttagttc attctgaaga taaagctata
     3601 tttgaaagtt ttcttgaaag tattaaaaaa aagaatatac acagtcaagc agtatatcgt
     3661 ttaaaaaaat ctaatggtga atatatttgg tgtagaacat caattacaag catttataat
     3721 gatgaaaatg aacttttaag agttgtcgga gtaattgtag atgtagatag tgatataaaa
     3781 aaatttgaag agttaagaac tagagcagaa agtgactcat tgaccaatat atacaataaa
     3841 ggcacatttg aaaaattagt ggaagaaact atagttatga atacaggtga taaaaaagat
     3901 gcattattca ttattgattt agatgatttt aaagaaataa atgataattt tggtcatcca
     3961 tttggtgatt ttgtactgaa gacatttgct gataagatac aggctagttt tggtagtaaa
     4021 gacttagtcg gaagaattgg tggagatgaa tttgttgtat atatgcaaga ttacgttaca
     4081 gaagtaaatt tgcataaaaa agctaaagaa ttaaatagag tactttcaga taattacact
     4141 gatttgagtt ttagttttga tgcttctgtc agtataggta ttgcaaggta tcctcaagat
     4201 ggaacttcat tttttgaatt gtttaaaaat gcagatagag cactttatag tataaaagca
     4261 tcaggtaaaa attcatattg tttatttgaa gaagaactat atgtacagtg aatttgaatt
     4321 tagattttat gtgagagtaa gatttatata tacttgtggc tgtttaattt ataaaatatg
     4381 ttactttgta tatttttata tgtttcagtg aggttaatta acataagcaa aggtgtaagt
     4441 aaatttatat taatttaagt ttacttacac ctttataaaa ataaatttta taaatttaaa
     4501 cagttggttt tataaagatt tttatttatt ttaatatatt agctgttcct ttttgtactc
     4561 tttagagaag ataaataatg aaaggagtac agaaaaaatt aaacatccta atataacact
     4621 tgaattaagg cttgaagtat taaagtttaa aaaagcatta tttaaattgg tatccaaaag
     4681 aacagtatta ttaattaaat gaattactac acatgaccat acatttttag ttttcatata
     4741 gacataacca aagaatatag aaagactaat cacagaaaat aatctaagta atatacctaa
     4801 accatctaca tgataaattg taaaccatag tggtatgtgc cataattccc atataatccc
     4861 taataatata acacccattc gtttgccaaa ttttgtttgc attttttctt gaagaaaacc
     4921 tctccacccg tattcttctc caaagaaaag gatactttga tttgctatat atggaaaaat
     4981 taaaaatata ttcataatca aatttataaa attaatatct ttccatccta acattataca
     5041 tttacttaga ttaattatta ttataaaaat aaaaatacaa ataatagatt tatttacatt
     5101 cttaaatggg taaagctctt taccttctaa acatgcataa catatcgtta taattccagt
     5161 aaataagact aaaataccca ttcctaagat tacagtttca tcatctattg ctccaaatgc
     5221 ttttaaagta actagaaata gagataataa aaagtcagct attatgacac agtgaagtat
     5281 aatatgttca tcactctttt tatgtttata aatctttgct attgaaactc ctgatgctgg
     5341 taatatagtc ataaatgtta ccattatttg tggattcata tattctttaa aatatagagg
     5401 tatcccaaac agcagtgata taccataagt tactacaaaa aatattacaa cttctagagt
     5461 atttattttt ttataattac ttctatcaga tatctcgctt tgtatcataa taatttgtct
     5521 ccttttacat tttaacttct gctgagattt gagttagata ttcattagaa tctcgcacac
     5581 ttatatcata taaaagatat tcttcataaa caaaatcccc catacataaa ttattctgat
     5641 taaaaaaatt taataacttt tcataagttt tgtaaatagt ttcataactt cctttatgat
     5701 atgcagttac ataaagtccc tttgctttga ttgatacagg atatttcttg tactctttat
     5761 taacttttgt aaataaatat gaatatgatt caaaatcttt attaattata tcttcacctt
     5821 taatgattga gccaatcgta ccataatcac tcaactcata gttgtccata aagttaatac
     5881 attcatataa aaaattttct tcctcatctc ctaaaaactg ttctttatat ataataggcg
     5941 ttttgactaa gtactcttct tcatgttctt ttaaaataat agtattcgca tctatattac
     6001 aggcattttt agtaaatgaa atagtagatt ccattagagc ttgaatatta tttagttttt
     6061 caatttcatt tttaatatca atagttttct tttctaaaag ctcaattagt aaattaggtg
     6121 ttcttttgtc tatataagtt ttaatttctt ttaaaggcat ttttacttcc ctaagtgaag
     6181 aaattatacc aaaaatgcta agttgatggt atgagtagta tctatatcca ttttctttct
     6241 ttaattctgg tgagaaaaga cctatatcgt cataatggaa aagtgttttt ttattgattc
     6301 cacaaatctt tgcaaattct ccagttgtaa aataattttt tgaaacttga ctcataatta
     6361 taattcctcc ttgactatcc tgttacagta tagtttatca taatattttg taaaggagtg
     6421 tgattgtatg aaaaaattaa ttcatttttt aaaaccatat agagttttaa ttgttatcgt
     6481 tttgatattt acgtttttac aaacacttgg aactttgtat ataccaacac ttacagcaaa
     6541 tatagtaaac aatggtgtag taaagggaga tatagattat attgtaaaaa caggtttaat
     6601 gatgatgata gtagctggta taacagcttt atcagctgta ttagtttgta aagtatcagc
     6661 aaatctttca tctggatttt gtagagatat tagagaagcg gtatttgtaa aatcacaaga
     6721 tttatcaata aatgatttta ataatatagg tacagcatca atgattacta ggagtactag
     6781 tgacattact ttaataggtc agtcagtgtt tatgtttatt caattagtct taccagcacc
     6841 aattataact gtttctggtc tatttttagc atattctata gataaagtaa tgacagttat
     6901 tattgttgta gtaatgtttc tattcatgtt atcagcattt ttagttggta aaaagcttat
     6961 caagttgttt aaaatgatgc aagtaaaaat ggacaatatg aatagggtgt tacgtgaggt
     7021 agtaactgga gtaagagtta taagagcttt taatagaagt cattttgaga aaaagagatt
     7081 tgatagaact gcaatagatt attctgaaac agcaatttca ataaataaaa tatttgcagt
     7141 acttatgcct attgtaatgt tgataatgaa tctaggtata gtaagtatta tttggtttgg
     7201 cgggatgaga gtaagtaatg gaaatatgga aataggtcat ataatggcac ttgttgaata
     7261 ttgtatctta attttattct atttaatcat gggagtaatg gtatttatgt acatacctag
     7321 agcaggtgca tgtgcagata gagtaaatca gatattagat atagaacctg aaattgtaga
     7381 tggaaatggt cgtgaagatg ttgtctcaga gagggggcat ttagtattta aaaatgtaac
     7441 attttcatat gcacaatcag aagaacctgt tttaaataat ataacatttg aagcaaagtc
     7501 tggagaagta actgcaatta taggaagcac tggctcaggc aaatctacta ttgcaaatat
     7561 aataccacgt ttttttgaaa ttcaatctgg agaaatatct ataaatggtc aggatattaa
     7621 gaagattcct caaaaagaac ttagagataa gattgggttt gtaccacaaa aagctttttt
     7681 atttagtggg acaatagaag aaaatataag gtatggaaaa gaagatgcaa gtattgaaga
     7741 agtaaagcat gctgcaagta tagctcaagc tgatgaattt atttctgata tggaagataa
     7801 gtatgactca tttgtagcac aaggtggaaa taatttgtca ggtggtcaaa aacagagaat
     7861 atccattgca agagcattag ttagaaagcc tgaagtatat gtctttgatg atagtttttc
     7921 tgcactagat tttaagacag ataaaagact tagaaaagcg ttgaagaatg aaatcaaaga
     7981 ttcatcagct ataattatag cacagcgtat aagcacaatc atggatgcta atcaaattat
     8041 agttttgaat gatggaaaaa ttgtcggtat tggaaaacat aaagatttac ttgaaaattg
     8101 tgaggtttat aagcaaattg cagattcaca acttagtaaa gaagagcttg cataggatgt
     8161 agaattaatc tgtgtatttg gaggtatgta atatgagtga aaaaagaaca gataaacaag
     8221 atatagaggt acttagtttt gaagatggta tgtcagcaga aaaagctaag gatgcaaaaa
     8281 aaactactaa gagattatta aaatatatgg caaaacaaaa gtttaaaata ttaattattt
     8341 ttatttcagt attgataagt tcagcactta cagttttggc gcctatggtt atgggaaaag
     8401 ctattgacca actatttaat ggaataaaaa ctgcagtcca aacaggtaca aaattcagtg
     8461 tgaattttag tactatggga ggtatagtat caatacttct agggttgtac ttaattagtg
     8521 cagtatttat atatattcaa cagttcatta tgtcaggagt tgcacaaaat ctagttctta
     8581 gcatgagaaa agatttaagt gataaactca ataaacttcc tcttaagtac tatgattctc
     8641 ataaaaaggg ggaaacactt agtatagtaa caaatgattt agaaaaagta gcagatagtt
     8701 tacaagaagg attaatgcaa cttattacag cagtagttac agtagttggt tctattgtaa
     8761 tgatgataag tataagcgta ccacttacaa tagtatcagc tataacatta cttgtttcat
     8821 tgggtattac tgttgtaatt gcaagaaaat cacaagaaag attctctgaa aatcaaaagg
     8881 cactaggaga gttaaatagc aatatagaag agatatttac tggtcaaata gtggttaaag
     8941 catttagtaa agaaaaggat actataagaa actttaaaat attaaatcaa aatctttata
     9001 atgcaagtag tagagcacag ttttcgtcat atgcaatatc tccaattatt agatttataa
     9061 acctaattgg ctatgttatt atagcagttg taggtggtat atttgcatca actggagcaa
     9121 tgactcttgg tagtatacaa gcgtttattc aatatgtaaa tcaagcatca gagcctacta
     9181 cagaaatatc ttatattgta aatatgcttc aagcagcaat agcatctgca gagagagttt
     9241 ttacagttat ggacgaggta gaagaaataa aggatgaaga atcttcaaag gttattagca
     9301 tgcctaaagg taaagtacaa tttgagcatg ttaaatttgg atatagtgat gattttattc
     9361 ttatgaaaga tattaatatc aatctaaatg caggagataa gattgcaatt gttggaccca
     9421 caggagctgg aaagacaact cttgtaaatt tacttatgag attttatgaa atacaaggtg
     9481 gaagaattac tattgatggt gttaatataa aagatttaaa gcgtggagaa cttagaacta
     9541 tgtttggtat ggttcttcaa gatacatggc tgtttaatgg aagtataaaa gaaaatattg
     9601 catatagtaa gttagatgcc acaatggatg aaattgtaag agcagcaaaa tcagcaagag
     9661 tagaccattt tataaggact ttaccacaag ggtatgacac tatattgact gaggatgctt
     9721 ctaatctatc tcaaggtcaa aagcaacttc taacaattgc cagggcaata ctttcagacc
     9781 catcagtatt gattcttgat gaagcaacat ctagtgtaga tactagaaca gaggtagaaa
     9841 ttcaaaaggc aatgaataac ttgatgaagg gaagaactag ttttgttatt gcacatagat
     9901 tatcaactat tcgagatgct gatttaatat tagtaatgaa agaaggcact attattgaac
     9961 aaggaagaca taaggaactt attgaaaaga aaggattcta tgaagaactt tataacagtc
    10021 aattttcaag tgaatatgat gaagatgttg tatgattatc aagttttagg taactaaatg
    10081 actgataaat attaagttaa atatagtaac atataaggtt atatggaata agagtatttt
    10141 tctatttcat ataacctttt ttatttgtca aaataagata aaatttcctt tctaaattag
    10201 attgaagaat gtatttatca atataaaagt aaaataaccc ttattattat tgaagtaaat
    10261 attgtttgcc acatatatgc cataaagatg cacttatttt acggagattt aacaaagtag
    10321 tgtaaagtat actctaaata taaaataaat caaatagagt taaataaatt gagaggtgat
    10381 ataaatagtg aataaaaaat tgaattatag atttgaaatg aagcacaaaa taactgaagc
    10441 agatgtgcta gctttaaaat caagactcta tccaacaatg aagaaagatg aaaatgcatc
    10501 taaagatgga aaatatctta ttaggagttt atattttgat actcctgaag ataaagcact
    10561 tttggataaa ttaaatggcg ttgcaacaag agaaaagttt aggataagat tttataataa
    10621 tgactgctct tatataagac tagagaaaaa aatcaaacat tacaacatga catcaaagct
    10681 atcagctagt ttaaccaaaa aagaagtaat aaatatacta aacaatgata ttgaatttct
    10741 aaaagagtcg acaaattgtt tattaagaga attttatttg aaattaaaat gtgaaagatt
    10801 agaggcaaaa agcatagttg attacaatag agaagctttt atttatccag ttggaaatgt
    10861 aagagttaca atcgatagtg atattaaaac ttctgtaaat tcggtagatt tatttaataa
    10921 agatttgcca actgtttcgg taattgatga gaatatgact gtattggagg ttaaatacga
    10981 tgaatttata ccagatttta taaaggattt aattcagatt aataagacta cttctacagc
    11041 agtttcaaag tatgcttcaa gtagattata tatttaagaa taaaggctgt ggtttatata
    11101 aagttataca taaataaggt taaaaaacaa ataaattaaa aataaataaa ataaaaaata
    11161 aataagttaa aaataggaag gatgatttta attatgacat ttaatgatat ttttaaatca
    11221 agttttatag aaaacgtatc aggtttttca tttgtggaca gtgctttagc attaggttca
    11281 gcttttttag taggtttatt tatatatatg gtctataaaa agacttatat gggaattatg
    11341 tattctcgac cattcaatgt gtcgcttgta gcacttacaa tgcttacaac ttttgtaata
    11401 ttggcggtta cttcaaatgt agttttatct ttaggtatgg ttggtgcttt atctatagta
    11461 agatttagaa cagcaattaa agacccaatg gatttagtat ttctattttg gtcattgggt
    11521 tcaggtatag ttttaggagc aggtttgatt cccttagctg taatgggttc aataattatg
    11581 ggattgattc taatattctt ttcaaacaaa acaatatctg aaactccata tatattgatg
    11641 gtaaattgta ataatgaaga ttcagaagat atagcaactg ataaaattaa aaaagtattt
    11701 actaaatatc aaataaaatc aaaaagtgtt acaccagaaa aaggaataga attagtgttt
    11761 gaagtaagaa tgaaagatgg agagacaagt cttataaatg acttgagtca agtagatgga
    11821 gttactaatg cagttcttgt aagttataat ggtgactatg tagcatagag aggagtaatc
    11881 ttttatgaaa gataaaaaat ttacccttct tatctcgatt atgattatat ttttatgtgc
    11941 tgtagttgga gtttatagta catctagcaa caaaggtgtt gatttataca gtgatgtata
    12001 tattgaaaaa tattttaaca gagacaaggt tatggaagtt aatatagaga tagatgaaag
    12061 tgacttgaag gatatgaatg aaaatgctat aaaagaagaa tttaaggttg caaaagtaac
    12121 tgtagatgga gatacatatg gaaatgtagg tataagaact aaaggaaatt caagtcttac
    12181 atctgtagca aatagtaata gtgatagata cagctataag attaattttg acaagtataa
    12241 tactagtcaa agtatggaag ggcttactca attaaatctt aataactgct actctgaccc
    12301 atcttatatg agagagtttt taacatatag tatttgtgag gaaatgggat tagcgactcc
    12361 agaatttgca tatgctaaag tctctataaa tggcgaatat catggtttgt atttggcagt
    12421 agaaggatta aaagaatctt atcttgaaaa taattttggt aatgtaactg gagacttata
    12481 taaatcagat gaaggaagct cgttgcaata taaaggagat gacccagaaa gttactcaaa
    12541 tttaatcgtt gaaagtgata aaaagaccgc tgattggtct aaagtcacaa aattattaaa
    12601 atctttggat acaggtgaag atattgaaaa atatcttgat gtagattctg ttcttaaaaa
    12661 tatagcaata aatacagctt tattaaacct tgatagctat caagggagtt ttgcccataa
    12721 ctattattta tatgagcaag atggagtatt ttctatgtta ccatgggatt ttaatatgtc
    12781 atttggtgga tttagtggtt ttggtggagg tagtcaatct atagcaattg atgaacctac
    12841 gacaggtaat ttagaagaca gacctctcat atcctcgtta ttaaaaaatg agacatacaa
    12901 aacaaaatac cataaatatc tagaagagat agtaacaaaa tacctagatt cagactactt
    12961 agagaatatg acaacaaaat tgcatgacat gatagcatca tatgtaaaag aagacccaac
    13021 agcattttat acttatgaag aatttgaaaa aaatataaca tcttcaattg aagattctag
    13081 tgataataag ggatttggta ataaagagtt taacaacaat aactctaata acagtgattc
    13141 taataataat tctaatagtg aaaataagcg ttctggaaat caaagtgatg aaaaagaagt
    13201 taatgctgaa ttaacatcaa gtatagtcaa aactaataca gataatgaaa ctaaaaataa
    13261 aactacaaat gatagtgaaa ataagaataa tacaaataaa gataaaagtg gaaatgataa
    13321 taatcaaaag ttaaaaggtc ctatgggtaa aggaggtaag tcaataccag gggttttgga
    13381 agttgcagaa gatatgagta aaactataaa atctcaattg agtggagaaa cttcttcgac
    13441 aaagcaaaac tctggtgatg aaaattcaag tggaattaaa ggtactgaaa agtttgatga
    13501 ggatatgagt ggtatgccag aaccacctga gggaatggat ggtaaaatgc caccaggaat
    13561 gggcaacatg gataagggag atatgaatgg taaaaatggc aatatgaata tggatagaaa
    13621 tcaaggtaat cctggaggtt ttggcaatag aggaggaggc tctatgagta aaacaacaat
    13681 atactccaaa ttaattttag gtggagcttc aatgataata atgtcgatta tgttagtagg
    13741 tgtatcaagg gtaaagagaa gaagatttat aaagtcaaaa taagattaaa attgaatata
    13801 aataatttta atactgttaa gtctaaatat ttacttagca gtattttttt agaaaataat
    13861 gtgtttga
//
LOCUS       DAEMAE010000091        13853 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEMAE010000091 DAEMAE010000000
VERSION     DAEMAE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.093"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            64..1665
                     /locus_tag="KPH93_002100"
     CDS             64..1665
                     /locus_tag="KPH93_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG1688920.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKIV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSAGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1884..3470
                     /locus_tag="KPH93_002101"
     CDS             1884..3470
                     /locus_tag="KPH93_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG1688921.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQKNSIPSDTEKMLKDVKKA
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSINVEVNVLEYKTNDMNSSEF
                     KRMVSSEIYSLVNSYRKEKGKEALKVSEDLQGMANYWSKYMADKGEFAHVINGKNAAE
                     VFSGGMRSEENIAFVPLTTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSNFAYTG
                     FGLYILPDGQVYATQEFLNK"
     gene            3674..5818
                     /gene="slpA"
                     /locus_tag="KPH93_002102"
     CDS             3674..5818
                     /gene="slpA"
                     /locus_tag="KPH93_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG1688922.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADSTTPGYTVVKNDWKKAVKQ
                     LQDGLKNKTISTIKVSFNGNSVGEVTPASSGAKKADRDAAAEKLYNLVNTQLDKLGDG
                     DYVDFEVTYNLATQIITKAEAEAVLTKLQQYNDKVLINSATDTVKGMVSDTQVDSKNV
                     AANPLKVSDMYTIPSAITGSDDSGYSIAKPTEKTTSLLYGTVGDATAGKAITVDTASN
                     EVFAGNGKVIDYNKSFKATVQGDGTVKTSGVVLKDASDMAATGTIKVRVTSAKEESID
                     VDSSSYISAENLAKKYVFNPKEVSEAYNAIVALQNDGIESDLVQLVNGKYQVIFYPEG
                     KRLETKSADIIADADSPAKITIKANKLKDLKDYVDDLKTYNNTYSNVVTVAGEDRIET
                     AIELSSKYYNSDDKNAITDDAVNNIVLVGSTSIVDGLVASPLASEKTAPLLLTSKDKL
                     DSSVKSEIKRVMNLKSDTGINTSKKVYLAGGVNSISKDVENELKNMGLKVTRLSGEDR
                     YETSLAIADEIGLDNDKAFVVGGTGLADAMSIAPVASQLKDGDATPIVVVDGKAKEIS
                     DDAKSFLGTSDVDIIGGKNSVSKEIEESIDSATGKTPDRISGDDRQATNAEVLKEDDY
                     FKDGEVVNYFVAKDGSTKEDQLVDALAAAPIAGRFKESPAPIILATDTLSSDQNVAVS
                     KAVPKDGGTNLVQVGKGIASSVINKMKDLLDM"
     gene            6064..8409
                     /gene="secA"
                     /locus_tag="KPH93_002103"
     CDS             6064..8409
                     /gene="secA"
                     /locus_tag="KPH93_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG1688923.1"
                     /translation="MSVIDSILDKADEQEIKKLNVIVDKIDALEDSMKNLSDEELKDM
                     TAIFKNRLKKGETLDDILPEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMSPVYESLGMTVGVIISNQD
                     PNIRKQQYKCDITYGTNSEFGFDYLRDNMVPDLSYKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSRMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLKQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            8824..10695
                     /locus_tag="KPH93_002104"
     CDS             8824..10695
                     /locus_tag="KPH93_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG1688924.1"
                     /translation="MNKKNLSVIMATTMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSRTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYVT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10802..11509
                     /locus_tag="KPH93_002105"
     CDS             10802..11509
                     /locus_tag="KPH93_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG1688925.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIENNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            11542..13374
                     /locus_tag="KPH93_002106"
     CDS             11542..13374
                     /locus_tag="KPH93_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp66"
                     /protein_id="HBG1688926.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSDNGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTKNKNDLIDSLAVGV
                     LAAKNSSPIVLAGNKFDTTQKDVLNTKTIDKVTQIGGLGNENVVEDILDIQEETKYTV
                     ETIDELNAAIKRADANDIIKFKPEKEKTINNSFSIETKKTVTIELDGRYRQTITLDIP
                     NGKFNNYAEIEGGVKLKNIKNESLVNKGSIQDLDIYDENGCKIENESSGEIWFVTIVE
                     EANDVYIVNSGDITKISNNSSGTIIRNSGNIDTVTGKKEPAISGNKPKVNDTEKETKA
                     ARGLNPRVEACSVPKKDYVMITIPNSPKDSRYKIYYRVVYNKPYAMDVGDKINIGEWT
                     VAPTDEEPFLEKAKNGCYVEAVEVNTSTKEVSRWGRTNVTDDGF"
     gene            complement(13421..13822)
                     /locus_tag="KPH93_002107"
     CDS             complement(13421..13822)
                     /locus_tag="KPH93_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG1688927.1"
                     /translation="MILKKYKETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKVFTNIVVIVLNYLFSKMIIFKNKK"
BASE COUNT         5772 a         1528 c         2593 g         3960 t
ORIGIN      
        1 aagagaaaat aaatggaaaa taattacaat ataaacataa aatataaaaa tcatcagggg
       61 gatatgaaaa tgaacaaaaa aatattatca ttaggtctag cagtatcatt aatcttagta
      121 aacttcaaaa gtgtaaatgc atcatcagta gtagaaaaaa tatacggtaa agatagatat
      181 gaaacagcag caaagatagc tgataaacag acttatgaaa cagtgatttt agtaaataca
      241 gaaaaatcac ttgcagatgg attaagtgca agtggattat cgggggctac aaaagctcca
      301 atactattta cacaacaaaa taagatacca gcagacacaa atagatgtct aaaaaatatc
      361 aaaaaagcat atataatagg tacagaagat actataagta aatcagtaga aaaagaacta
      421 gattctaaaa atatagaagt caaaagaatt ggtggagaag atagactaaa aacaagctat
      481 ttaatagcta aagaaatagc aactataaaa aaagtagata aagtactatt aactaatgca
      541 tatagtggag aagcagatgc aatgagtgta tcatcagtag ctactagaga tggagctcca
      601 atcatactta cagatggaaa gagtgtgcct tttgatgtaa aaaatattca atcatattgt
      661 ataggttcag aggagataat gagtaatcct ttagttaaaa atacaaactc agtaagaata
      721 gaaggaactg accgatttga aactaacaaa aacgtaatag actacttttt taatagtgca
      781 gatggattct atgtatcaga tggataccaa ttagtagatg caatagcagc cgcaccactt
      841 actaaaaact ctccaatggt actagtaaat gatggaagcg ataagattgt attagaagga
      901 gctaagaata taacctctgt aggtgaaata aatgagaagg taatacaaca gtgtataaat
      961 gcttcaaagt caaatggaca acccccaaca attacagttg ggagtacaga ggtatataag
     1021 ggtgaaaagt ttgacactgg caagttaaat atagtggcta aagataacac tgggaaggta
     1081 ttaccaatag aagttgatgg gtttatagat acaaatagag taggtacata catattgaca
     1141 ttaaaagcta cagacgaatg gggaaaaagc gcaggaaaaa gagtagaaat aaaagtgttg
     1201 gatgataaat ctcatgatta caacagccca gaatttaaaa aaatggtatc tactgaaatg
     1261 tataacttaa tcaattccta tagaaaagaa aaaggcaaag agcctttggt agtatctagt
     1321 aggctagaag gtatggcaaa tgcatggtct aagtatatga tggataaaaa agtatttgca
     1381 cattatatag atggaaaaaa tgctccacaa gtattttctg agtttggaat gagaagtgag
     1441 gaaaatatag catatattta tatagattct aagaacgttc aaacgacaca agatgcaaaa
     1501 gacttggcaa aggctatatt tgaagtgtgg aagaaatctc cagagtacaa tgcaaacatg
     1561 cttagtgatg agttctatag tactggattt gggctttata tattatctga tggtcaagtg
     1621 catgctactc aagagttttt aaatggcaat gaaggtagct tgtaaaaaat tccataaatc
     1681 ttgttttttt gttaatttac tgatattata aaatgaaaat cacatctaaa gtactttttg
     1741 taatctgttt tgctattgct ttacaggtta tggtatttta ggtgtttttt gttttagtag
     1801 tttataaatt ttgagggaat cactactgag gatgtaattt atgattagct gaattatata
     1861 aaaaaattag taggaggagt ttgatgaagg taaataagag agtattatca ataggattga
     1921 caatatcatt aataatggca ggagcaccaa atatcaatgc actttcaagt atagaaaaga
     1981 tacaggggaa agatagatat gagacagctg ccaaaattgc acagaaacaa acatatgaaa
     2041 atgtagtatt agtaaataca gataatacac tagctgatgg attaagtgca agtggattag
     2101 caggaacagt taaagcacct atattattat cacaaaaaaa cagtatacca tcagacacag
     2161 aaaaaatgtt aaaagatgta aagaaggctt atataatagg aactgaagat tcaataggaa
     2221 aatcagttga aaatgaatta aagcaaaaag ggatagaagt taaaagaata ggtggtaatg
     2281 atagaattga aacaagttat cttatagcaa aagaaatagc ttcaataaaa cctatagata
     2341 aggtttttat aactaatggt tatacaggtg aagcagatgc tatgagtgcg tcatcagtag
     2401 catcaagaga tggagcacct ataattttaa ctaatggtaa aaatgttcca tttgagaaaa
     2461 aagaaggtgt gcaatgttat gctcttggtt cagaggagat aataagtaat gatttagtta
     2521 aaaaaactaa tgcagttaga ttagctggag aagatagatt tgagactaat aaaaaagtta
     2581 taaaacattt ttatagttca gcaaaagaat tttatctttc taaaggttat caattagtag
     2641 atgcagttgc tggttcttct atagcaaaaa atgcacctat tgtattggtt gatggaaata
     2701 gtgataagtc tgttcttaga agtgctgata aaatcacagc actaggagga atagatgaaa
     2761 agacgctaga acaatgttta agtgcatcat cactggatgc aagtgcacca acaataactg
     2821 ttggaaattt aaatatttat caaggtgata agtttgatat aagtaaactt aatatagtag
     2881 caaaggatag caatggtaac gatttgacac cagagcttat aggaaatatt aatactgata
     2941 aggtgggtaa atacaaagtt actataaaag ctactgatat tggtggaaag actacctcaa
     3001 taaatgtaga agtgaatgta cttgaatata agactaacga tatgaatagt agtgagttta
     3061 aaagaatggt ttctagtgaa atatatagtc tggtaaattc atatagaaaa gaaaaaggta
     3121 aagaggcatt aaaagtttct gaagatttac aaggaatggc aaattattgg tctaagtata
     3181 tggctgataa aggtgaattt gcgcatgtaa taaatggtaa aaatgctgct gaagtatttt
     3241 ctggtgggat gagaagtgaa gaaaatatag cttttgtacc acttactaca aagagtactt
     3301 atacaacaaa agatgctagg gaagtggcaa atgtcatatt tacagtttgg aagaagtctg
     3361 ataagtataa tgaaaatatg ttgaatagca actttgcata tactggattt ggactataca
     3421 tattaccaga cggacaagtg tatgctactc aagaattttt aaataaataa aattaaagtt
     3481 ttatttcatt agattattat ggtataaaaa cttatatatt tggagaaaat aataatttaa
     3541 aaaaatagct tgcaaaaaga ataaaaatgg attattatag agatgtgaga aatattagga
     3601 atatatggat gattattcta tgtacataat aaagagatgt aattttaata taatgttggg
     3661 aggaatttaa gaaatgaata agaaaaactt agcaatggct atggcagcag ttactgttgt
     3721 gggttctgca gcgccaatat ttgcagatag tactacgcca ggttatactg tagtgaaaaa
     3781 tgattggaaa aaagcagtaa aacaattaca agatgggttg aaaaataaaa ctatatcaac
     3841 aataaaggtg tcttttaatg gaaactctgt tggagaagtt acaccagcca gttctggagc
     3901 aaaaaaagca gatagagatg ctgcagctga aaagttatat aatttagtaa atacacaatt
     3961 agataaacta ggtgatggag attacgttga ctttgaagta acttataatt tagctactca
     4021 aataattaca aaagcagaag cagaggcagt tcttacaaaa ttacaacaat ataatgataa
     4081 agtacttata aattctgcaa cagatacagt aaaaggtatg gtatctgata cacaagttga
     4141 tagcaaaaat gttgcagcta acccacttaa agttagtgat atgtatacaa taccatctgc
     4201 tattactgga agtgatgatt ctgggtatag tattgctaaa ccaacagaaa agactacaag
     4261 tttattgtat ggtacggttg gtgatgcaac tgcaggtaaa gcaataacag tagatacagc
     4321 ttcaaatgaa gtttttgctg gaaatggaaa ggttattgac tacaataaat cattcaaagc
     4381 aactgtacaa ggagatggaa cagttaagac aagcggggtt gtacttaaag atgcaagtga
     4441 tatggctgca acaggtacta taaaagttag agttacaagt gcaaaagaag aatctattga
     4501 tgtggattca agttcatata ttagtgctga aaatttagct aaaaaatatg tatttaatcc
     4561 taaagaggtt tctgaagctt ataatgcaat agttgcatta caaaatgatg gaatagaatc
     4621 tgatttagta caattagtta atggaaaata tcaagttatt ttctatccag aaggaaaaag
     4681 attagaaact aaatctgcag atataatagc tgatgcagat agtccagcta aaataactat
     4741 aaaagctaat aaattaaaag atttaaaaga ttatgtagat gatttaaaaa catacaataa
     4801 tacttactca aatgttgtaa cagtagcagg agaagataga atagaaactg ctatagaatt
     4861 aagtagtaaa tattataatt ctgatgataa aaatgcaata actgatgatg cagttaataa
     4921 tatagtatta gttggatcta catctatagt tgatggtctt gttgcatcac cattagcttc
     4981 agaaaaaaca gctccattat tattaacttc aaaagataaa ttagattcat cagtaaaatc
     5041 tgagataaaa agagttatga acttaaagag tgatactggt ataaatactt ctaaaaaagt
     5101 ttatttagct ggtggagtta attctatatc taaagatgta gaaaatgaat tgaaaaatat
     5161 gggccttaaa gttactagat tatcaggaga agacagatac gaaacttctt tagcaatagc
     5221 tgatgaaata ggtcttgata atgataaagc atttgtagtt ggtggtactg gattagcaga
     5281 tgctatgagt atagctccag ttgcttctca acttaaagat ggagatgcta ctccaatagt
     5341 agttgtagat ggaaaagcaa aagaaataag tgatgatgct aagagtttct taggaacttc
     5401 tgatgttgat ataataggtg gaaaaaatag cgtatctaaa gagattgaag agtcaataga
     5461 tagtgcaact ggaaaaactc cagatagaat aagtggagat gacagacaag caactaatgc
     5521 tgaagtttta aaagaagatg attatttcaa agatggtgaa gttgtgaatt actttgttgc
     5581 aaaagatggt tctactaaag aagatcaatt agtagatgca ttagcagcag caccaatagc
     5641 aggtagattt aaggagtctc cagctccaat catactagct actgatactt tatcttctga
     5701 ccaaaatgta gctgtaagta aagcagttcc taaagatggt ggaactaact tagttcaagt
     5761 aggtaaaggt atagcttctt cagttataaa caaaatgaaa gatttattag atatgtaata
     5821 taagttttaa taaaacctta aatagaaaaa ggcttctctc atgagaagtc tttttctatt
     5881 taaaataaat ataaaataaa atagaggcta tttatagcct ctattttatg tgagaaatac
     5941 ttaaataaaa agatgtttaa tttatttaag taaaatcatt atactataaa tcaaaataat
     6001 tgtctactat tatggatttt taatgtataa tagtaattgg acaatagaaa aggaggtact
     6061 tatatgtcag ttatagattc gatacttgac aaagcagatg agcaagaaat aaagaaattg
     6121 aatgttatag tagataaaat agatgcatta gaagatagta tgaaaaatct atcagatgaa
     6181 gaactaaaag acatgacagc tatatttaaa aatagactta aaaaaggtga aaccttagat
     6241 gatatacttc cagaagcatt tgctgtagta agagaagtat caaaaagaaa attgggaatg
     6301 cgtcaatata gagttcaatt aattggtggt atagtaatac atcaaggaaa gatagcagag
     6361 atgaaaacag gtgaaggtaa aactctagtt gaagtagcac cagtatattt aaatgccctt
     6421 acaggtaagg gtgtacatgt aatcacagta aatgattacc tggcagaacg tgataaggag
     6481 cttatgagcc cagtttatga atctcttggt atgacagtag gagtaattat atctaaccaa
     6541 gacccaaata taagaaaaca acaatataaa tgtgatataa cttatggtac aaatagtgaa
     6601 tttggatttg attatttaag agataatatg gtgccagatt tatcttataa agtacaaaga
     6661 gaactaaact ttgccatagt agatgaggta gactcaatat taatagatga agctagaact
     6721 ccacttatta ttgcaggaga tggtgatgaa gatttaaagc tttatgagtt agcaaatagc
     6781 tttataaaaa ctgtaaaaga agaagacttt gagatggaca gaaaagataa aactatagca
     6841 ttaacagcaa gtggtataag taaagctgag tcattttttg gtataacaaa ccttactgat
     6901 ataaagaaca tagaattata tcatcatata aatcaagctt taagaggtca taagcttatg
     6961 gaaaaagatg ttgactatgt tatctcaaat ggagaagtaa tgatagttga cgaatttaca
     7021 ggaagagtaa tggatggaag aagatataca gatggacttc accaagctat agaagccaaa
     7081 gaaggtgttg agataaagaa tgaatctaag actatggcta ctgttactta tcaaaatttc
     7141 ttcaggctat atgaaaaact ttctggaatg actggtactg caaagacaga agaaggggaa
     7201 tttgagtcaa tctataaatt aaatgttgtc caaataccaa ctaataaacc agtgattaga
     7261 gcagatttac atgataaggt atttaaaaca gaagaagaaa agtatagtgc tgttgtagaa
     7321 gaaataataa ggatacataa gactagacag ccaatacttg taggaacagt ttctgttgaa
     7381 aaatctgaga agctgtctag aatgcttaaa aaacaaggta ttaagcatca agtgttaaat
     7441 gctaaacaac atgataaaga ggcagagata atttctaaag ctggtaaatt agatgctata
     7501 acgattgcta caaatatggc aggtagagga accgatattt ctctaggtgc aggagataaa
     7561 gaagaagaac aagaggttaa agatttaggt ggactttatg ttataggaac agaaagacac
     7621 gaatcaagaa gaattgataa ccagcttaga gggcgttctg gtcgtcaagg agatccaggt
     7681 acatcaagat tctttgtaag tcttgaagat gatgtaataa agctttatgg tggaaaaact
     7741 atagagaaac ttatgaagag aacaagttca aaggaaaata ctgctattga aagtaaagca
     7801 cttacaaggg ctatagaaag agcacaaaaa ggtgtagaag gtaaaaattt tgaaataaga
     7861 aaaaatgttc ttaaatatga tgatactatt aatgagcaaa gaaaagttat atataatgaa
     7921 agaaataaag tgttaaatga tgaagatata caagaagata ttcaaaaaat ggttaaagac
     7981 atcatacaag aagcaggaga aacttattta attggaagaa aaagagatta ctatggatat
     8041 tttaaacatt tatatagtac atttatgcca gcagatacat tactaatacc tggtgtagat
     8101 aaaaaaagtg tccaagagat aattgatagc acatatgaaa tttcaaaaag agtttatgac
     8161 ttgaaaaaga tgatgattgg tattgataag gttgcggagt tagaaaaaac agtactttta
     8221 aaagtggttg atcaatactg gatagatcat atagatgcta tggaacagtt aaaacagtat
     8281 ataggtctta aatcttatgc tcaaaaagac ccatttaagg aatatgcttt agaaggatat
     8341 gacatgtttg aagctttaaa taaaaatata agggaagcaa cagtgcaata cttatataaa
     8401 tttaactaaa aaagttcatg taatttttat taaatgaaaa ataaagtatt ttgattcttt
     8461 atattgtaat tacaattaaa taattaagga atacaaatga aaaagaagct gaaaatcagc
     8521 ttctttttct gtgtgagaaa tacggaaaaa agagatgctt gaaatttctt tcttccattt
     8581 aatattatac tataaagaag atgaaaagtc tattagttat aaactggatt attaaaaaaa
     8641 attacaattt tgttactttt ttgtatacat aaatattaaa aaaataaaat ataagaaaaa
     8701 gtttgtatct tttggttaat tattacaata agtctcattt attgaaataa tatcaaatat
     8761 atattataat tggtaaataa ggaaaaaata ataaaaattt gaatttttta gggggaaaat
     8821 accatgaata aaaaaaatct ttctgtaatt atggctacta caatgataag tacatcagta
     8881 gctccagttt ttgctgcaga aactacacag gtaaaaaaag aaacaataac taagaaagaa
     8941 gctacagaac tagtttcgaa agttagagat ttaatgtctc aaaagtatac aggaggttct
     9001 caagttggac aaccaatata tgaaataaaa gttggtgaga ctttatcaaa attaaaaata
     9061 ataactaata tagatgaatt agagaaatta gtaaatgctt tgggagaaaa taaagagctt
     9121 attgtaacta taacagataa agggcatata acaaatagtg caaatgaagt agttgcagaa
     9181 gcaactgaaa aatatgaaaa ttcagcagac ctttccgctg aagctaactc tataacagaa
     9241 aaatctaaaa ctgaaactaa tggaatttat aaagttgcag atgtaaaagc ttcatatgat
     9301 agtgctaaag ataagttagt tataacttta agagataaaa cagaaacagt aacttctaga
     9361 actatatatg taggtattgg tgatgaaaaa gttgatttaa cagcaaatcc agttgattca
     9421 actggaacaa acttagaccc ttctgcagaa ggatttagag taaataaaat cgataaatta
     9481 ggtgtagcag gagctaaaaa tattgatgat gttcaattgg ctgaaataac tataaaaaat
     9541 agtgacctaa atacagtttc accacaagat ttatatgatg gatatagatt gactgttaaa
     9601 ggtaatatgg tagcaaatgg tacatcaaag tcaattagtg atatttcagc aaaagattca
     9661 gaaacaggaa aatataaatt tactattaag tatactgatg catctggaaa agcaacagag
     9721 cttactgtag agagtactaa tgaaaaagat ttaaaagatg ccaaagctgc attagaaggt
     9781 aattcaaagg ttaaattgat agctggagat gatagatatg taactgcagt ggctatagca
     9841 aaacaaacaa aatatactga caatatagtt atagttaatt caaataaact agttgatgga
     9901 ttagcagcta caccacttgc tcaatctaaa aaagcaccta tattattagc atctgataat
     9961 gaaataccaa aagtaacttt agattatata aaagatataa ttaagaaaag cccatcagct
    10021 aaaatatata tagtaggagg agaatcagca gtatcaaata cagctaaaaa gcaattagaa
    10081 tcagtaacta agaatgttga aagactagct ggagatgata gacatacgac ttctgtagca
    10141 gtggcaaaag ctatggggtc ttttaaagat gcatttgtag taggtgcaaa aggggaggct
    10201 gatgctatga gtatagctgc taaagctgct gaacttaagg ctcctataat agtaaatggt
    10261 tggaatgatc tttcagcaga tgctatcaaa ttgatggatg gaaaagagat tggcatagtt
    10321 ggtggttcta acaatgtatc tagtcaaatt gaaaatcaac ttgctgatat tgataaagat
    10381 agaaaagttc aaagagttga aggagaaaca agacacgata ccaatgctaa ggttatagaa
    10441 acatattatg gcaaattaga taaactatat atagcaaaag atggatatgg aaataatggt
    10501 atgctagtag atgcattggc agcaggacct ctagcagcag ggaaaggtcc aatacttcta
    10561 gctaaaactg acataacaga ctcacaaaag aatgcactta gtaaaaaatt aaatcttggt
    10621 gcagaagtga ctcaaatagg taatggagtt gaattgacag taatacagaa gatagctaaa
    10681 atattaggtt ggtaataatt tatactgtgg agaagattcc ttaattaata caggaatctt
    10741 tttctataat tatgctattt attgtaaatt ataacatttt ataaagtagg aggatgtagt
    10801 agtggtagat gcaactttat tttttacacc acatcaagat gatgaaacct taagtatggg
    10861 aagtgctata attgaacatg ttgaaaaaag tgatacacat gtcatattat gcacagatgg
    10921 gagtaaatct ataataagaa aagttttgga tgatggaggg tcatgttctt atcatattaa
    10981 agatgttcat aaatactctt tatcagaaag tgaattttca aaagatagag atgaagaatt
    11041 taaggacagc tgtgaagcaa tgggtgtaaa agaaagtaat atacatatag aaaataatag
    11101 agcccatgat ggtgagctaa gtaaagaaaa ggcaagggaa ataatactta agtacttaga
    11161 agaatatcca gatgcaaaag taaaaactgt tactccgttt aaagctagtg gaatacatga
    11221 agaccataga gcattaggag aggctgcatt agagttatat agagaaggca aaataaaaga
    11281 tttaagattt tatgtagagc catatgacta taaggatttt aaaaaagtta atccaaatgt
    11341 agaagtatgg aaggtattac ctagtcaaga ggagaagtta ttaagtgcaa tgaatgcata
    11401 taaaaaatgg aatcctgaaa gtggacatta tgctatagga tatcattcag taaaatctca
    11461 ttttgatgag ttagctacaa ataaaataca gtatgtacat gctccataaa atattacgaa
    11521 agaattagga ggtaagaatt aatgaaaata tcaaaaaaga tagtgtcttt gttaactatg
    11581 acatttttaa ctgttacatt atatggaaat acatctaatg catctacaaa agacacatta
    11641 acaggttctg gaagatggga aacagcaata aaaataagtc aagcaggatg gaaaaagtct
    11701 gaaaatgctg tgctagtaaa tgataattca atagcggatg ctttatcagc tactccattt
    11761 gctaaagcaa aagatgcgcc aatattatta actcaaagta ataaattgga tagtagaaca
    11821 aaagcagaat taaaaagact tggtgtgaaa aatgtatatt taataggtgg ttcaattgca
    11881 ttaagttcag agattgaaaa gcagttaaat gcagaaaata taaattttga aagaatatct
    11941 ggaaatagta gatatgatac ttctttaaaa ttagctgaaa agctagatag ggaaaaatct
    12001 atatctaaaa tagtagtagt aaatggagaa aagggtcttg ctgatgcagt gagtgttgga
    12061 gctatagctg cccaagaaaa tatgccaata atactttctg attcagataa tggaactgaa
    12121 gtagccgata attttataga tagtaaggat atagaaaaat cgtatgtaat aggtggtaca
    12181 tattctattt ctaattctgt agaaagaagt ttaccaaatg caacaagaat agcaggtagt
    12241 agtagaagtg agacaaatgc aaaaattata gaagaatttt ataaagatac tgatataaaa
    12301 aatatttatg taacaaaaga tggtacaaag aataagaatg atttaataga ttctttagca
    12361 gtgggtgtat tagcagctaa aaatagttct ccaatagtat tagcaggaaa taagtttgat
    12421 actacccaaa aagatgtgtt aaatactaaa actatagata aagttactca aattggtggt
    12481 ttaggtaatg aaaatgttgt agaagatata ctagatatac aagaagagac taagtatact
    12541 gttgaaacta ttgatgaact taatgctgct ataaaaagag cagatgcaaa tgatattata
    12601 aagtttaaac cagagaaaga aaaaactata aataattctt ttagtattga aactaaaaaa
    12661 actgttacta ttgaattaga tggaagatat agacaaacaa taactttgga tatacctaat
    12721 ggaaaattta ataactatgc agaaatagaa ggtggagtaa agctaaaaaa cataaaaaat
    12781 gaatcattag ttaataaagg aagtatacag gatttagata tatatgatga aaatggttgt
    12841 aaaatagaga atgaaagttc tggagaaatt tggtttgtca ctatagttga agaagctaat
    12901 gatgtatata tagttaatag tggtgatata acaaaaatat caaataattc ttctggtact
    12961 ataataagaa attctggaaa tattgataca gttacaggaa aaaaagaacc tgcaataagt
    13021 ggaaataaac ctaaagtcaa tgatacagaa aaagaaacta aagctgctag aggcttaaat
    13081 cctagggtag aagcatgttc tgttcctaaa aaagattatg ttatgataac aattcctaat
    13141 tcacctaaag attcaagata taaaatttac tatagagtag tttataataa accttatgca
    13201 atggatgttg gtgataaaat taatattggc gaatggactg ttgcaccaac agatgaagag
    13261 ccatttcttg aaaaagctaa aaatggttgt tatgtagaag ctgttgaggt aaatacttca
    13321 acgaaagagg tttctagatg gggaagaact aacgttacag atgatggatt ttaaatatta
    13381 aaatctttta aaaacaaaag gtagacaatt ttttgattgt ctacttttta tttttaaaaa
    13441 ttatcatttt actaaacaaa taatttaaaa ctataacaac tatattagta aatactttta
    13501 caataacatc atttacaaaa agcaaatcaa tcattacata cataattaac atttcaacga
    13561 caccagaaag tagcctaaaa ctaacaaaag acaaaaattc tttaaataag aactttattt
    13621 caattctttt actttcaaac acaaaaaact tattagtaac ataagcaaat aaaacagcaa
    13681 gtatccatgc caaagcattt gcaaccataa aattaaataa aataactcta gtaaaaaata
    13741 aatacgaaac tatatttaca agagtagtaa atgctccaaa aaataaatat aatattgtct
    13801 ctttatactt ttttaaaatc aaatataatc ctcctaagct aatttaatat aaa
//
LOCUS       DAEMAE010000092        13757 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEMAE010000092 DAEMAE010000000
VERSION     DAEMAE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.094"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1120
                     /locus_tag="KPH93_002108"
     CDS             <1..1120
                     /locus_tag="KPH93_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1688928.1"
                     /translation="YKSNYDVNIYKCLIVLFSYWAILVCINALSMIVIVSINNLSSMN
                     ILLNDNFYRYETIVLGNVVLIVLLCIYKFISNDLQVTKKEIYMCIPIVANMISFFIFY
                     KYILKLLDISSATRLDILIMVLLLTFSNIVLISSLVKILKSNKKYIELTKLQEKINAE
                     NKYYSNIKKYYIKTKELNHDMKNHLICIKEMNKKNIDTSNYINNIEKKLDEYSAVFDT
                     GNITLNIILLEKKELCIEKGIDFTININFSDCNFIKEEDTCSIFANALDNAIEACEKI
                     KDDDKKIKLQGMVLNKFFVIKITNTKSNDILLKNNAFLTTKEDKEFHGLGIKSIKNSL
                     EKYDGAISINFSENLFCLNMMIPIRKSQEDSNIAYENS"
     gene            1163..1744
                     /locus_tag="KPH93_002109"
     CDS             1163..1744
                     /locus_tag="KPH93_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG1688929.1"
                     /translation="MIKSCADKIAFLFVLNQVIEKDDLEICSYGLEILLSSIINTLSS
                     LMIGLILGRLIYSIVFLISFCIIRQFSGGYHANSNKNCILIFNSIFFISMVLGTILNN
                     SNPIPLFLVILSSFNFLIIFIFAPVCHTNNPLNETKYKKNKLLSRTISITLFLIILLG
                     CKYLSFYNYVVYTFLALLWINLLLIIQIIINKK"
     gene            1762..1923
                     /locus_tag="KPH93_002110"
     CDS             1762..1923
                     /locus_tag="KPH93_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG1688930.1"
                     /translation="MKKILYNFMKACGVLAMFVAFLSANTTSLWHVYQPKTPEKLKNS
                     KNHQKEGNN"
     gene            complement(1945..2742)
                     /locus_tag="KPH93_002111"
     CDS             complement(1945..2742)
                     /locus_tag="KPH93_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1688931.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCQIANKQNVDLAVQIHFNANKTTLNPMGTETICKTNNGKVY
                     AERVNKKLSTIFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKKIDNDGVKLMYKHAIVYDGEVDKIPATVVGWGYNDGKILICDIKDYI
                     PGQTENLYVVGGGACEKIGSMAKENYTLIKGNGRFDTLYKALDFIDR"
     gene            complement(2739..2999)
                     /locus_tag="KPH93_002112"
     CDS             complement(2739..2999)
                     /locus_tag="KPH93_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG1688932.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYISVILLAL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGEKQ"
     repeat_region   3296..3584
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3430..3452
                     /rpt_unit_seq="attccatatagttaatataaaac"
     gene            complement(3869..4027)
                     /locus_tag="KPH93_002113"
     CDS             complement(3869..4027)
                     /locus_tag="KPH93_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688933.1"
                     /translation="MDNFLQGILASLSASLIVYVASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(4385..5755)
                     /locus_tag="KPH93_002114"
     CDS             complement(4385..5755)
                     /locus_tag="KPH93_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG1688934.1"
                     /translation="MELKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINTDSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAASVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYITSGTDLVYALVASPMSKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6037..6135
                     /locus_tag="KPH93_002115"
                     /pseudo
     CDS             6037..6135
                     /locus_tag="KPH93_002115"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6154..6576
                     /locus_tag="KPH93_002116"
     CDS             6154..6576
                     /locus_tag="KPH93_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688935.1"
                     /translation="MTFVERCYKYIDMNKNNWSPYTVTNTKSWVKNYIEPFFKNLKLT
                     EVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVISEVYRLREIQENSCDFIKLPSKEK
                     YSTNEIYNREEVLLLIEKLLGNNIEIPILLMLLLGLRM"
     gene            6894..7079
                     /locus_tag="KPH93_002117"
     CDS             6894..7079
                     /locus_tag="KPH93_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1688936.1"
                     /translation="MRIHDLRHTSATLLLLGGTNMKVVSERLGHTDIKITMNRYSHVL
                     KEMDKEASDNLSELLFK"
     gene            complement(7442..7522)
                     /locus_tag="KPH93_002118"
                     /pseudo
     CDS             complement(7442..7522)
                     /locus_tag="KPH93_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7562..8230)
                     /locus_tag="KPH93_002119"
     CDS             complement(7562..8230)
                     /locus_tag="KPH93_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG1688937.1"
                     /translation="METVSNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGISLIV
                     IFKKDSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNISFI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDINDLITNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(8329..8712)
                     /locus_tag="KPH93_002120"
     CDS             complement(8329..8712)
                     /locus_tag="KPH93_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1688938.1"
                     /translation="MSQKELAKKIGVSTSQLIKIISRKTRMVSSGILIGVADIFKFGL
                     SEDIIKELVIGAVDVEILNRLMEHKNFQCLINLMRIYSQDMVDLGDMERNQFIDMVTI
                     FLSNLMKESPKYYTEVRKELTDGIK"
     gene            complement(9112..11532)
                     /locus_tag="KPH93_002121"
     CDS             complement(9112..11532)
                     /locus_tag="KPH93_002121"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG1688939.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVKKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMIDSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNVVVK"
     gene            complement(11886..12236)
                     /gene="rsfS"
                     /locus_tag="KPH93_002122"
     CDS             complement(11886..12236)
                     /gene="rsfS"
                     /locus_tag="KPH93_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG1688940.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(12353..12925)
                     /gene="yqeK"
                     /locus_tag="KPH93_002123"
     CDS             complement(12353..12925)
                     /gene="yqeK"
                     /locus_tag="KPH93_002123"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG1688941.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(12926..13615)
                     /locus_tag="KPH93_002124"
     CDS             complement(12926..13615)
                     /locus_tag="KPH93_002124"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG1688942.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4692 a         2266 c         1582 g         5217 t
ORIGIN      
        1 ttataaatca aattatgatg ttaatatata caaatgttta atagttttgt tttcttactg
       61 ggcaatactg gtgtgtataa atgcattaag tatgatagta atagtttcga taaataattt
      121 gagttctatg aatattttat taaatgataa tttttataga tacgaaacta tagttttagg
      181 aaatgtagtt ctaattgttt tgttatgcat ttataaattc atatctaatg atttgcaagt
      241 gactaaaaaa gaaatttata tgtgtatacc aatagtagca aatatgatat cattttttat
      301 attttataaa tatatcttaa aacttctaga tatttcttcc gctaccagat tagatatatt
      361 aattatggta ctattattaa cattttctaa tatagtattg atttctagcc tagttaaaat
      421 attaaaaagt aataaaaaat atattgagtt aactaaatta caggaaaaaa taaatgctga
      481 aaataaatat tatagtaata taaaaaaata ttatattaag acaaaagaat taaaccatga
      541 tatgaaaaat catcttattt gcattaagga aatgaataaa aagaatattg ataccagtaa
      601 ttacataaat aacatagaaa aaaagcttga tgaatatagt gctgtatttg atacaggaaa
      661 tattacttta aacattatat tattagaaaa aaaagaactt tgtatagaaa aaggaattga
      721 ttttacaatt aatataaact ttagtgattg taattttatt aaggaagaag atacatgttc
      781 tatatttgca aatgcattag ataatgcaat cgaagcttgt gaaaaaatca aagatgatga
      841 taaaaaaatc aaattgcagg gaatggtatt aaataaattc tttgtaataa aaattactaa
      901 tacaaaatca aatgatattt tattaaaaaa taatgctttt ttaactacaa aagaagataa
      961 agagtttcat ggattaggaa taaaaagtat aaaaaattct ttagaaaaat atgatggtgc
     1021 aatttctata aatttctctg aaaatttatt ttgtttaaat atgatgatac caattagaaa
     1081 atcacaagaa gattcaaata ttgcatatga gaatagttaa tatccataat ttattaataa
     1141 aattatttaa ggagaaaact aaatgataaa atcttgtgct gataaaatag cttttttgtt
     1201 tgttttaaat caagtaatag aaaaagatga tttagaaatc tgttcatatg gattagaaat
     1261 tttactatct tccataataa atactctatc ttcattaatg ataggtttaa tattaggtag
     1321 attgatttat tctatagtat tcttaatatc tttttgtata ataagacaat tttcaggtgg
     1381 atatcatgca aatagcaata aaaactgtat actaattttc aatagtattt tttttatctc
     1441 tatggttttg ggaacgattt taaataattc taatcctatt ccgttatttt tagtgatatt
     1501 gtctagtttt aactttctaa tcatttttat ttttgctcca gtatgtcaca ctaacaatcc
     1561 actgaatgaa acaaaatata aaaaaaataa gttactgtca agaactatat ctataacttt
     1621 attcttgatt attcttttag gatgcaaata cttatctttt tacaattatg ttgtatacac
     1681 ttttttagct ttactttgga taaatttatt actaattatt caaatcatta taaataaaaa
     1741 ataatttaag gagatactaa catgaaaaag attctatata attttatgaa agcgtgtggt
     1801 gtattagcta tgtttgtagc atttttatct gcaaacacaa cttctctgtg gcatgtgtat
     1861 caaccaaaaa caccagaaaa actaaaaaat tctaaaaacc accaaaaaga aggtaacaac
     1921 taactctagt tgctaccttc caatctatct atcaataaaa tctaatgctt tgtaaagtgt
     1981 atcaaatcta ccattacctt ttatcagggt ataattttct ttagccatgg aacctatctt
     2041 ctcacatgct ccaccaccaa caacatataa attttctgtc tgacctggta tataatcttt
     2101 tatatcacat attaatattt ttccatcatt ataaccccaa ccaactacag ttgcagggat
     2161 tttgtcaact tctccatcat aaacgattgc atgtttgtac atcagtttaa ctccatcatt
     2221 atctattttt ttatttaaaa taccttccgc tattaactta gcaactatgt ctttatgtct
     2281 gatataatag tctgtatctg ctttactatc tacgaaacac acttctatta atattgcagg
     2341 agcttttgta tgactaagcc agtaaagacc tcttacatcc gattttgcac ctctattttt
     2401 aaatattgtt gatagttttt tgttgactct ttcagcatat accttaccat tgttagtttt
     2461 gcatattgtc tctgttccca taggatttag agttgtttta tttgcgttga aatggatttg
     2521 tactgctaag tctacatttt gcttattggc tatttgacat tgctctgcta aatagttatt
     2581 agatttatct acctttccag tatatacagt agctccacct tgtttcaacc atttaacaat
     2641 taaatcagtt aaaattctgt tttcttttcc ttcatctata tagccagttg ctcctgttcc
     2701 ttttccactt aaagtgtgtc ctggtactat tgctattttc attgcttttc accatccttc
     2761 aactgtttgt aaacttgatt tgctcctatt gcaactcccc aacataaaat tccttgcaaa
     2821 attgaacttg gattaaaccc tagcatccat attgaaaata ctataccaag tgctagcagt
     2881 ataactgaga tatatttatt atctaactgt ttgtatttct taaatccaaa tcctaataca
     2941 ttaagagcaa ctactaataa aagtagttgc tctggtataa aacttattaa attatccata
     3001 tcttaacctc ttaattaatt aaaatattcc tctttgaact gcaaatataa aaaaacctat
     3061 taaagtagta atcattggcg tttgggtgat tttttatttt ctcgtaaatg taagctgaca
     3121 caattccagc tactatactc aataaaaagt taattaacat ataaattcac ctccttccta
     3181 tattggaatt tggcgtttat atgagaaaat caccctcagc ttttcaactt tattttcctt
     3241 gctacaatta ttatatcata taattcttac atattttaac aaatatttca attaagtaca
     3301 caggtttgaa tataaaaaag cacctaccac aaaagtaagt gtttttcagt atttttaatt
     3361 ttaagtccat atagttaata taaaacctta aagctacata ataaaaaatt aagaaaaaga
     3421 aaaatttaca ttccatatag ttaatataaa accattcata atcaaaaatg acaatacagt
     3481 atggtcatga tttacattcc atatagttaa tataaaacta cattatataa gatagctaaa
     3541 aatcttttag gcaaatttac attccatata gttaatataa aaccccaaaa taaattgaat
     3601 atttccaatg cctacacata tacaaatctc tcaaatttgc agtgaaccat gagtagtgca
     3661 attgataaca cttatcacaa gtcctcaatg ccttatatct caactgttaa gccttaattt
     3721 cttcaaaaaa tcgaacactg caaaatttct atgactttat tatatcataa aaatattatt
     3781 tttgaatatc tgtaccaatt tgtgatataa taaaagcaag aagagctaca atctattttg
     3841 ctgtagagtg gagttcataa tttagaaatt acttagtttt atggaatctg atttttaaat
     3901 caaattccca accactctta gttgccgctt tgagtggttt tttacgcttt ctaaatagtt
     3961 tgctagctac ataaactatt aagctagcag atagacttgc tagtatgcct tgtaaaaaat
     4021 tatccatgta tattcacctc ccttcttaac gtcgggagga taattttttg atacatgaac
     4081 tccactctat aaattgtaga ttacatcttc ttgctaaaaa tattataaca tataattttt
     4141 acatatttta cctatttatt actttttaca ttataataat attcttttaa tcaaaaaaac
     4201 acctactatt aaagtaaatg tttttaagta tttttaattt taaatccata tagttcatat
     4261 gaaactaaat ataataactc aacatatgag caatatcgag acatttacat accacatagt
     4321 taatataaaa cgaaaataaa aaaattaaac ttataaaacc cttattaatt tacataccca
     4381 ctagttattt atattcaaaa aaatcttctc catctgggtt ataaaataat attctacttg
     4441 ccaatccttg ttcatattta taattttctt tatcacctac ataagaaaat gttaatgtca
     4501 tgcttcttga tggatctaat gaaattatat ctaaataaat agtacatttt tctcctggct
     4561 ttaatacacc gaaactatta aaatccattt ttattttagc atattgcccc atatataatg
     4621 ttgaattagc ttctccataa acttttaatt ttgttacatc taaattttgt gtacttgtat
     4681 tttctatatt tataggaact gttgggtctt tatatttttc agtataatat tcaaggttat
     4741 catcataaca ttcaagataa cttcctttat cttgccaaat tatgtgcttt gaatcaaaat
     4801 caatctttaa tccacatatt atatttttat ttgaagatgg aagactaata ttattgatgg
     4861 tattttcaca ctctaacata attttatcac ttagatttcc tatcccggtt acttttgcag
     4921 catttttaag tatatcttta ttgcttccaa tatcaactag cacaatagga ttatttttag
     4981 acattggtga agcaactaaa gcataaacta aatcagtacc actagttata taaaattctt
     5041 ttgcgccatt atagaactta tttactattt ttttattagt atcatatcta tctactccgc
     5101 ctaatctagt tgaattagta tcattaacta gtttgtcact cattgatgaa gtaccaccta
     5161 ttacataact ttctacacca gttgtattaa atggaacact ttttccatca gttaatacta
     5221 tagctgcttt atctctaact gctacagatg cagcactcat tgcatcaggc tctcctttaa
     5281 aagcattagt caatataact ttatttactt tgttaattga gtttatttct tttgcaacat
     5341 tatagcttgt cttaattcta tcactacctt gaagtctttt agcctctatt cctttatctt
     5401 ttagaagagt ttctgttgct ttatcaatag aactttctcc acctattatg tatacctttt
     5461 tagctttctc tactctttta agagttgcat taggtatgtt atttttctta gttaaaagaa
     5521 ttggtgcatt tgttgctcct gcaagtccac tcgcacttaa tccatcagcc attgtgctgt
     5581 ctgtattaat taatatagct gtagtataat tttgttcatc agctataaga gcagccgttt
     5641 catatttatc ttttccttga atagtataaa ttgtatcaag tgcattagct gatataggac
     5701 cagcagcaaa cattgacacg gctaacccta gtgataataa tttctttaat tccacaatat
     5761 ctccaccttt tatatatgaa atactacata tcttaataat tctacattaa attttctttt
     5821 cctcctttaa cgatatttat cttcctgtta atttaagtgt atacaaatac gtgttataat
     5881 gtaactgtaa agaattaaaa agataatgac ataattagag caatccaact gttcttttat
     5941 aaaactaact tataaagata gcgtacatat atgaatatca aatcagcttt tataagaaaa
     6001 agatgggaaa aattctatgt atatgtggaa taagaaactg gcaaaaagaa acaaaaaagt
     6061 tatggaagct atgaaaagaa aaaggatgct gaaaaacatt taattgaaat aaagtctact
     6121 ataaataata ataagtttgt tgctccaaaa ggtatgacat ttgtagaaag atgttataaa
     6181 tatatagaca tgaataaaaa taattggtct ccatatactg ttacaaatac aaaatcttgg
     6241 gttaaaaatt acatagaacc tttttttaag aatctaaaac tcacagaagt aaatcctagt
     6301 tcagtacaag cttttgtaaa ttataacttt aataattcta cctttactag tgctaaagta
     6361 aggtatagct ttctaagttc tgtaataagt gaagtgtata gattaagaga aatacaagaa
     6421 aattcatgtg attttataaa acttccttct aaagaaaaat attctacaaa tgaaatatac
     6481 aatagagaag aagtattatt gctaatagaa aagttacttg gtaataatat agaaatacct
     6541 attcttttaa tgttacttct aggtttaaga atgtaagaag ctactggact taggtggtca
     6601 gatgttgact tagataacaa tctgataaat gttaatcaaa ttcttgtcta ttctaatggc
     6661 aaaatatctt ttaaagagcc aaaaacacca aaatcaaaaa gagctttatc agctccaaaa
     6721 gaattaattg aaaaattgaa aattgaaaaa ctaagacaaa gcaaattaaa attgcaaggt
     6781 atcctggaaa acagaaataa cttagtttgt ctaaacacca atttaaatcc gtttctacct
     6841 tcttcactaa gaaaaacgtt tcacaatttt acaaaaaaaa taattaaaaa aagattagaa
     6901 tacatgattt aagacataca agtgccactc tgctgctttt agggggaact aatatgaaag
     6961 ttgtttctga aagattgggt catacagata taaaaataac tatgaataga tattctcatg
     7021 tacttaaaga aatggacaaa gaagcttctg acaatctaag cgaacttcta tttaaatagt
     7081 aaaaactgac tgtcagttaa atgtcagtta aacctaaaaa aataggtttt gtcagttgat
     7141 tgtcaatcaa ttattttata atagtttcac ttaaaaacgt ctatatctac acattttact
     7201 atttgtttat gcttattaat atctatctgt tattattaat tctaatagct tctatttaat
     7261 tatttagttc agtttacaag aagctacaca caaaccataa aataggatgt tgacaagata
     7321 acagacagta tgctcctgta aaaggtaaca tactatctgt tatgttgtct ggatttccaa
     7381 atggttaccc tttgtcacac gactttgcta tctccttggt aaactgtaag tttgtaatct
     7441 aaaatcctaa tccatctcta taaaatttaa ttgacttctc catacttttt acacctaaag
     7501 atataaaagt aattttattc acttaatgat ctccataatt cccaaattaa ctatttggaa
     7561 cttaactaaa aaatcccaat ataagggcaa ctattataat ggcaattgga atacatactg
     7621 tataatcctg ttccatagag tcatcatctg aatgtagttt tacaattcgc aatttagcaa
     7681 tcagtttgaa gcagaaatag cctattattg ttccaattac attcgtaatt aagtcgttta
     7741 tatcggttgc tctgtgtaac gtaaaaagct gaatagtttc gataaagagg gataacccaa
     7801 atccaattag aaatgtattt ctcaaacgtt caaaagtttt gcttataagc ggacataaaa
     7861 aacctaatgg gacaaaaagt aaaatattca aaataaaact caaactaaat ccatcactaa
     7921 aaggaataaa acttatgttt ggattaaaga acgtttcccc taactgtgac agtcttatat
     7981 attcactgaa tgtagggata ccaacaatat gcgatagtgt taaacataag tagtaataca
     8041 ttgccagtga taaaatagat gttttgacct ttggaatatc actatctttc ttgaatatta
     8101 caattaagga aataccaatc aagataattc ccaacaagaa aataggatta attaacaata
     8161 aatctttgat agcttcaaac agactgagtt gaatttctac acctgattga ttatttgata
     8221 cagtctccat ttgtacatgc ctccttataa catttcaatt atttctaaaa aacaaaatag
     8281 cagataaatc ttaaaatcat actatcaaaa ttcttaaaat tgcatttatc attttatacc
     8341 atcagttaat tcctttctta cttctgtata gtattttggg gattctttca tcaaattaga
     8401 caaaaaaata gtcaccatat caataaactg gttacgctcc atatctccca aatcaaccat
     8461 atcctgagaa taaatccgca tcagatttat caggcattgg aagtttttat gctccatcag
     8521 acggttgaga atctccacat ctactgcacc tatcacaagt tctttgataa tatcctcaga
     8581 caatccaaac ttgaatatat cggcaacacc tattagaata ccgctgctga ccatccttgt
     8641 ttttctgctg ataattttaa tcaactggga agtggaaacg cctatctttt ttgcaagttc
     8701 cttttgagaa atatggtagc cattatatta atctgaattt cgttgtctgg gtgtttcaga
     8761 gaaagccata gatatgcgct ttttttgcct gttttcatta tataaaaaaa tttcaaaaat
     8821 acaatgatta catttttttc atctaagctt aggatagttc ctcgataaac aatactttaa
     8881 aaactaaatg ctttacatac cacttaatac attaacgcga tttaacatta attataataa
     8941 tgtttaaaac aacaattagg atgtataaga atgaactttt tagttcttta atcatttttt
     9001 tgtataaaat caaatccatt tttataattt caacaatgaa aaaaggctta tctctatttt
     9061 gagacagcct catttatttt ataaatttat atttttgttt atatttttaa attatttaac
     9121 aactacattg actaattttt taggtacagc tacaacttta actatagttt taccttcaac
     9181 taatcctttt attttttcat cttctaaagc taccttttcc atctcatctt tagatatatt
     9241 tgagttaaca gttaattttc ctctaacttt tccattaact tgtacaacaa cttctatttc
     9301 atcttttact aaagcttttt cgtcatattt aggccagtct atgtcaaata cacttccttc
     9361 tttacctatc atagtccata gttcttcacc taaatgaggt gcaaatggtg caattatagt
     9421 tactatagtt tgtacacctt cttttataac tgcttcgttt atattatcta actctttata
     9481 tttatacata tcatttatta attccataag agctgatata gcagtattaa atccgaattt
     9541 ttcacttaaa tcagcagtaa cttttttaag tgtagagtgt atagtatatc tcatatcttt
     9601 atcttgagaa tttaattctc caaactctac atccttttta accacgtctg ctagctcgtc
     9661 aaccaatcta taaaccctat ttaagaatct aaagcatcca tcaacacctt gctctgacca
     9721 gtctaaatct ctttctggtg gtgccgcaaa taatacaaaa agtcttgcag tatctgctcc
     9781 atactcatct attatatcca taggagatac tgtatttcct ttagacttac tcattttaga
     9841 accatccata agtaccattc cttgagtaag taagttttta aatggctcat tgaagtctac
     9901 cattcccata ctcttaaatg cctttacaaa ccatcttgca taaagtaaat gcattatagc
     9961 atgttctact ccacctatat attggtctac tggatgccat ctattaacta attccttact
    10021 aaatggttca ttttcattat tgctatccac atatcttaag aaataccaag aagaatcaac
    10081 gaatgtatcc atagtatcaa cttcccttct agctggcttt ccacagtgag gacaagttgt
    10141 tgacataaat tgctttgaag tagtaagtgg agattctccc tttccagtaa attcaacatc
    10201 tgttggtaaa agaactggta aatcttcctt tttaactgga actattccac attcatcaca
    10261 ataaaccaca ggtataggac aaccccagta tctttgtctt gaaactaacc agtctcttaa
    10321 tctataatta attgttctct ttcctctacc ttctttttct aacttatcta ttattccttc
    10381 aaatccttct gaagcatcta gtccattaaa ctcttctgag tctatcattt tgttatcttc
    10441 tgttattaca tcaattatgt ctaaattata tttttcagca aaatctctat ctctttcatc
    10501 atgtgctgga actgccatta tagcacctgt tccatactca actaatacat aattagctat
    10561 ccataaagga actttcttgc catttaaagg atttataaca tatctaccta taaacattcc
    10621 ttctttttct acatcagccg ctgttctttc tatttctgtc atggtatgca tttttttaac
    10681 aaaagcttca acatctgctt catattcagt ccctgcaacc aattctttaa ctaattcatg
    10741 ctctggagca agtaccatat aagtaactcc ataagttgtg tctggtctag ttgtaaatac
    10801 tgtcatagat ttatcagttc catctatatc aaaaactaaa tctgctcctg tacttttacc
    10861 tatccaattt ttttgcattg ttttaacttt ttctggccat ccatctaatg tatctaagtc
    10921 attaagtaat tcttctgcaa attcagtagt cttaaaatac cattgttcta agtctttctt
    10981 taatactgtt gctttacatc tctcacatgc tccttgaaca acttgctcat ttgcaagaac
    11041 agtctcacaa gaaggacacc agttaacata cgattttttc ttatatgcaa gcccatgctc
    11101 taaaaacttc aaaaatattt cttgtgtaaa tctatagtat tctggagttg aagttgcaac
    11161 ttctttatcc caatcataac taatacctaa taaatttaat tgctctttca tttcttctat
    11221 attttccatt gtccatttat gtggatgtat tccatgtttt atagctgcat tttcagctgg
    11281 taatccaaaa gaatcccatc ccattggatg aagtacatta tagccttcca tttttttgaa
    11341 tcttgcgaca acatcaccta tagaataatt tctaacatgt cccatgtgta tttttccaga
    11401 tggataaggg aacatttcta atgtgtaata attaggtttt tcagtttgtg cagtatccat
    11461 tttatactga ttattatctt tccaaatttt ttgccatttc gattctactt ctttaaaatt
    11521 atatacgctc atattttgcc tcctattttt aattgtgtat aactacctta tttagtatta
    11581 tttacttata atttccaaat aattctttga aaataaaaaa tcctcgtcac taaacttacg
    11641 tttagggacg aggataaatc ttcgcggtac caccctaatt agcctactat atacatagac
    11701 cctcttagat agcttttaat ccagccatgg agaatagatt ttaatctagt aagttcaaag
    11761 ttatataata ttagttctca ccttccacta actctctgaa aaaatataat cttttactgc
    11821 tctagtctat tcaatttaat ataatttttg aaattttcta attataataa catatattat
    11881 catgtttatg ctaatgtgtc aatatctata taaggtgcat ctttccataa tttttctaaa
    11941 ttataaaacc ctctattttc ctcattaaat acatgaacca ttatgtctcc ataatcaagg
    12001 agtacccaag tttgagttcc ttgtccttct ttacctcttg gctctaatcc tagttttgct
    12061 aattcatctt ccacattgtc agcaatagct tttacctgtc tctgagaaga tgctgtagta
    12121 ataataaaat aatcacacaa actagaaact tttcctatat ttattataac tgtatcttga
    12181 cccagtttat cttctatggc atcatatgct atttttgtca tttgttccac tgtcatataa
    12241 aaattcctca ctttcctaat taaaatacac ttagttattg taacatatta atacgatttt
    12301 gtaacatagt caaatttaaa tttattaata tgtttgcctt tatatagctt ttttataata
    12361 acttttcttt aattagatag tttcttgcca taactgttct tggatgtatc tcttcttttt
    12421 tattgatgac aaacattaaa gtattattaa aagaagttaa aagtgcttca tcaagcttcc
    12481 ctccatatgc taattctctt aatgtatcta caactgggaa ttttcttcct tcttcaatta
    12541 aatcagctat gtaaattatt ttttctaaaa gagacatgtc ctctctccct gttgtatgat
    12601 atttgattgc atttataata tcttcatctt ctacttcaaa ctcatactcg catatgtacg
    12661 ccccaattac actatgagac aaggaacgat ttccatcctc tattgggtct aaatatattt
    12721 catacttatt tacataatcc tctatttcct tgtcacttaa atactttgca caatcatgaa
    12781 tcattcctgc taagtaagct ttttctttgt cacatccata aatctcacat aatttttcag
    12841 cacattttgc aacatttttt gaatggctaa gcctccccac tggtagcatt tgatttagtc
    12901 tctggttaat attttctaag ttcatttaat catctccata ttgatatagt ttattttcat
    12961 ataaataatt ttctacattt tcgggtacta agtaacgtat tgacttgcct tcatttagct
    13021 gttcccttat atatgtggac gatatatcca gtgatggaac ataaactgtt ataatgtcag
    13081 cattatactt cttagttagt ttttctatag tctcttgtga ccttaacaaa ctaattcctg
    13141 gtctagttgc agctataaaa gtagctaact caaagttttt ctttacatct ttccactctt
    13201 caacactaca aattgcatct gcacctgtta taaaataaat gtctgcattt ttgtattttt
    13261 tctttaagta ttttaatgta tctattgtat aagtcctttt ctttctttcg atttctactt
    13321 ttgaaactaa aaaatcttcg ttgctttcag ttgctagaag tgtcatatta tatctatcat
    13381 acttatctgt agtgatacat aatttatgtg gtggatttcc tgatggtata aagattattt
    13441 tgtcaatgtc atacttgtct ctaataaatt ctgctgttgc taaatgagca tagtgtatag
    13501 gatcaaaagt acctcccaat atacctattt tgattttgcc tttatggcga ttaaatttct
    13561 ctagtttttc agtatttttc aactctgcca tcttaactag attttcactt ctcatatttt
    13621 gtctcctttt aatgttataa tactattgta ccattatttt ttatttctta aagcttataa
    13681 tttgtttttt tgggtatata attatattta tatgctaaaa aacactttaa aaaataatta
    13741 tttaacatga ttctact
//
LOCUS       DAEMAE010000093        13584 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEMAE010000093 DAEMAE010000000
VERSION     DAEMAE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.095"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(236..1903)
                     /locus_tag="KPH93_002125"
     CDS             complement(236..1903)
                     /locus_tag="KPH93_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG1688943.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFIPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1977..2732)
                     /locus_tag="KPH93_002126"
     CDS             complement(1977..2732)
                     /locus_tag="KPH93_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG1688944.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIESELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2742..3278)
                     /gene="lepB"
                     /locus_tag="KPH93_002127"
     CDS             complement(2742..3278)
                     /gene="lepB"
                     /locus_tag="KPH93_002127"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG1688945.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3444..3980)
                     /gene="lepB"
                     /locus_tag="KPH93_002128"
     CDS             complement(3444..3980)
                     /gene="lepB"
                     /locus_tag="KPH93_002128"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG1688946.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4080..5390)
                     /locus_tag="KPH93_002129"
     CDS             complement(4080..5390)
                     /locus_tag="KPH93_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG1688947.1"
                     /translation="MTELPKKFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KRLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKILDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFNKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5469..6185)
                     /locus_tag="KPH93_002130"
     CDS             complement(5469..6185)
                     /locus_tag="KPH93_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG1688948.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6430..7701
                     /gene="sleC"
                     /locus_tag="KPH93_002131"
     CDS             6430..7701
                     /gene="sleC"
                     /locus_tag="KPH93_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG1688949.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8172..8828)
                     /locus_tag="KPH93_002132"
     CDS             complement(8172..8828)
                     /locus_tag="KPH93_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688950.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10041..10451)
                     /locus_tag="KPH93_002133"
     CDS             complement(10041..10451)
                     /locus_tag="KPH93_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688951.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIKNEDGSQISIAYNYAAID"
     gene            complement(10667..12049)
                     /locus_tag="KPH93_002134"
     CDS             complement(10667..12049)
                     /locus_tag="KPH93_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1688952.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSAGKNNSDEFEIMSSIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKLESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSTSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(12060..12419)
                     /locus_tag="KPH93_002135"
     CDS             complement(12060..12419)
                     /locus_tag="KPH93_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1688953.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSNDEITKLKQIVEKLK"
     gene            complement(12624..13403)
                     /locus_tag="KPH93_002136"
     CDS             complement(12624..13403)
                     /locus_tag="KPH93_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG1688954.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALLVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFILNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4619 a         2438 c         1346 g         5181 t
ORIGIN      
        1 tactctttta aaaaattatc acataaacga acaaaatatc aaacaaaaaa gtaaaattta
       61 aaataaaaag cttgcttcat acaaaagtat aaaacaagcc tttttatata tcaactcaaa
      121 ctttaaaatc tgtatcaatt taattctcca cttgacactt aatatacttc acataaacca
      181 atcacactac ttgctatacc atttaatctt cataaaacaa tctataatac acatattaat
      241 caactatttt acttcctcca ataaattccc taagaattga aatacctggt atgtcagaag
      301 tatcgtccaa ttcaacaaaa tactgcaaaa actttagtaa tctagtagct tcaatatgtg
      361 caggattatc ttcctttatc tcttccaaca aaggtttcgc aaatcgtttt aaaactgcca
      421 attcgtatac acaagcagag ttaaatatag catctgcctc ctcttggtac gggaatatat
      481 tcttcttttc tcctcttcta actgatgccc attgtaaaat agtattatca gcactatatc
      541 ctctaaaatt atagtctcta acaattcttc ttatcattct caaatcagca gtagggattc
      601 gattatggtc atccaaattt atttgagtaa gggcacttat gtatatctta aatttatctt
      661 catctggtat agatgatgtt aaaataggat ttagcccatg tataccttct aatataattg
      721 gctgatttga atctatcttt atctttttac cagtgtattc tctcttacct tctttaaaat
      781 tgaatcttat atcatcaatt tcttcaccag ctaaaagttt ctttaagtct aagttaaact
      841 tctctaaatc tattgcataa attgattcaa aatcataatt tccatattca tctaaaggag
      901 tatgttttct atctataaaa taatcatcta aagaaattga aataggattt aaattattta
      961 ctctaagatg tattgataat ctgtgagcaa aacttgtttt tccagatgaa gatggagctg
     1021 ctattaaaat tactctctta ttgccatcct ttatcatatc cgctatttgg gacaacttct
     1081 tttcatgcaa tgcttcaaat gtccttatta tatctccata ttcattgttt tctattattt
     1141 tattaagaga tactaccttg tcaattccca taagtcttga ccattcttcc gcttcataat
     1201 atacacttga tagcttaggt tgaggtataa acttcatagg aagagtctta tcttcttcac
     1261 taggccctaa aattataacg cctccattat aaagcttaat atcaaatgat tttatataac
     1321 cagttgatgg caacatataa ccataaaaat ggtctatata gtttctacac ttatatactt
     1381 tagcactatc atactctttg tatttcaaaa gctctgcctt ttcatacatt tcattatttt
     1441 caaagataga aatagcttca tttcttgtaa cttcaatttt ttctataaca taatcgttgt
     1501 ttactatctc tttcatcttg ttttttatat tttcaacatc tgcttcttta agctttctat
     1561 ctatatgaac ttcacaatat agcccatctg atagtgaatg ttctattgta actctgctat
     1621 cccaaaagat ttctctacaa gccattacaa atataaacga catgactcta aagtaaactc
     1681 tctctccatc ttcattagta gtatctaaaa aatttatctc acaatccttc ttaactctac
     1741 aatttagctc ttttaattta ttgtctacaa ctgcaagtgt tatataaccc ttatgctcat
     1801 cacaaaattc ttttgctata tctcccaatc tgattccagg ggaattttct tctaatgaat
     1861 attccttatc ttttccttct atttttaagt ttattttttt cacttcaaca tctccttcct
     1921 tttgatgaat ataaatttta attaatgagg catatcaaca agataaagag aggtatttaa
     1981 ccctctccta caatattatt caatttactt atagtatctt caatgtcttc gtaatttatt
     2041 tctaatacta aatattctgc atctaatgtt aagacctctt taatcccctc ttcagataaa
     2101 attcctcttc ctataccaac atgtgtatca ttgataccat cattatcact taagtgtatc
     2161 agtcttattt tatcctttaa gttctttaca taagcatcct catcaattaa acagtgtcca
     2221 gtatcgtaac aaaaacaaat cttagaattt ttaatcctct taaatatata ttcaaaatca
     2281 taagatatat ttccaatatt tgaaaaatcc ccaccttttg aataagtatt ttctatacat
     2341 acatatgaat ttgtatttaa tttgtctaaa aaatccacac tatttcctaa atatttgtcc
     2401 ctattctttc taactctatt tgtcataaca tatcccaaat gaaggttaaa ataccttata
     2461 tcaaatcctt ttagttcaga ttctatcttc tcaataaaat ttatccatga atttcgtata
     2521 tactctatgt tttcgcaagt atttaattcc ataggcaagt gtatccctat agataatcct
     2581 aacttagaaa ttctctcttt atatttacat aactcactac attcagatat attgtcaatt
     2641 ccaatctcta aatgagttat ttgctttata ctctcacaaa tgtttaaagc ctcctcaata
     2701 ttaaacaata aggatgctat accaactgcc atacaaatca cctactcaaa aatacctata
     2761 tctgtaaaag gaaatactct taaaataact tttcctttaa tattttcttc atctactagt
     2821 cccacttctt tatatctact gtctaaacta acttctctat tatctcccat cgcaaatact
     2881 tttcccttag gaatcaccat atcaatatcg ccttcagtat aattatcatg tatatatgaa
     2941 acttcattta ataacttacc atttatataa accttagaat ccttaatttt tagatggtca
     3001 ccaggaacac ctataaccct ttttacaagc tcttttttcc ttccattctc ttgtaataaa
     3061 tcagtcttaa atatcattat atctccacat tttggttctg ataacttata tgtcatcctg
     3121 tttgctacca aataatcatg tgatttaagt gttgggtaca ttgattctcc atttactagt
     3181 gttggtttta taaaacgagt tataataaat gcaaaaaaaa gtgcagtaat aattattttt
     3241 atccactcta caatctcttc tttaatagtt tcattcattc acaaattctc ctttatagtt
     3301 atattatctt tattgataat gtctcttaaa cactatcttt tagataagtc tactaaataa
     3361 ggtgttgtct tttttatata gacaactatt gtgtatggtc tggtgatttc agctaatcag
     3421 cgattgctcc aaattttata tgtttagtca atagttccta tcttagaaaa tggatacaat
     3481 ctgactagaa cctttcctaa aatggtatgc tcgtcgacca atccaacctc atcgtatcta
     3541 ctatctaaac ttttttctct attatctccc attgcaaata attttccttc tggaactacg
     3601 atatcaatat ctccatcagt acgattatta tgtatatagg aagtctcatc taacaattta
     3661 tcatttacat ataccttgga gtcttgtatt tttacatggt caccaggaac cccgataact
     3721 ctttttacta aatctttttt ctttccattt tcctctgtta agtcggtttt aaatactatt
     3781 atatctttgt attttggctc tcctaccttg tacgcaattc tattaactat caaataatca
     3841 cgttcaacta atgtaggata cattgattct cctttgacta ttgttggcac tataaaacga
     3901 gttattgcaa atgccaaaac aagagctatg acaatcactt ttatccattc tacaacttct
     3961 tttttaactg cttcacccac taaaatcgtc tcctctgtgt taaatattaa gtcaatctac
     4021 agcaaaataa tctaccataa acctacaata taaatattat acttcttttt tctaatagtt
     4081 taaaatactt ttctaagacc tttaggataa tgattcttaa ttacaccaga agattctttt
     4141 ccccaaccta aaggtattcc ttcaaccgaa accaaaaccc aacctctact atcattagaa
     4201 tttacaacat ccccttttaa atatttaaga attgattcac tttgtacact gaagttttca
     4261 acatttttta catcttcaac ttttaagtaa tgagatagtg catgagaagg ctcaaatcta
     4321 ttcttcttta aaacccctaa atgcaaaccg tatctcaata cttttagttt tttcgtatca
     4381 ggatatattt caggaagtag gtacaaattt tctcctctta aataaaattt atttttttta
     4441 ttactataaa aatcaacctt taaaaatttc ttttcaaact ccctataatc ctttatttct
     4501 ttatctaaat tttttatttt taattctttg acattgcatt cttcttcttc taattttttt
     4561 attttggcaa caaaatgacc ttctcccttt atcttatgag gccataatct ttccatttca
     4621 ataagttctg catgtggata ttcactgata aactcattta taacatgctc attttcttct
     4681 cttgaaaaag tacaagttga gtaaattaaa ataccatcct tctttaacat tttatatcca
     4741 tctcttataa tctctttttg gatggactga cattctacta cctttgcata actccaatcg
     4801 gatatggcaa cctcatcctt cctaaacata ccttgcccag aacaaggtgc atctataata
     4861 accttattaa aatacccatt aaacgtcttt cttaagctat tagaatcggt gtttgttata
     4921 atacaatttc tcgctccaaa tcgctctaaa ttttccccta gagccttaat tctactagca
     4981 ttaatttcat tagaaaccaa taaacctgta tcgtttagct tagatagtat atatgtagat
     5041 tttccaccag gagcagcaca catatcgaga attttttcac cctctttaat atctgccttt
     5101 ggtattacac tcattgcaga aggctcttgc aagtagtatg ctccagcctc atgcagtgga
     5161 tttctaccag gtctgtcaat tttatcatca taataaaaac cttcttctgc ccatggtatt
     5221 tgattcaaat caaacaactc caaatcaaat aatcttttct tgtcaatttt taaagtattt
     5281 attctaagcc cagtagtctt actttcctca taacttttta taaaatcatc atattcatct
     5341 tttagtattt cttcaatatc ttctaaaaat ttctttggta attctgtcaa atctataatc
     5401 ccccttcaat ctgctttact tgtgtgtcat ttattatact ttaattattt atgtctttac
     5461 cttaaatttt atttcttctt ctttttccta agattaccga aaaacacttt tttagagtga
     5521 ttcatatatt tctcaagttt atcaagattt atcttccaca aaattattac ataaactata
     5581 agtaatataa tagcccatct atattctatc atcaatatga aatcaaaaac tccatgtaat
     5641 aaaattggta ttaacactgc cataaacaaa tactcacgtc ttttattttt atttccctca
     5701 aatttatact tagaaatata gtagcccata gtaattgcaa acattacatg agctggtata
     5761 gagataactg ccctattaat accaacttgc agtgctagat ttctactttc agaaaaaata
     5821 taaaccatat tttcaattgt tgcaaatcct aaagctaaaa atacagagta aataatacca
     5881 tctaactttt cagtaaagtg tttttcttta attagggcag gaatcagtat cagagctttt
     5941 acaccttctt ctacaagtcc tgcaactaca aatgatacat atattagttc tgaataccct
     6001 tcaaatacat taaacttcat cagtaaattt tctaaaagta taattataac acttagaaaa
     6061 gcacctatta aaaaaaattt aattagatgg attagaggtt ctctacaata tctatcttta
     6121 gaccttatcc aaaacatccc tatcaatata ggaactattg ctaataaaaa taaatctagc
     6181 ttcatagatt attttccttt caaaattttt gatttattta tgatttatat catctactta
     6241 tttttaactt attcgtgttt taaatacata ctaaaatata cattaaatta ttttaaatta
     6301 tttaaataca aatatactca aatactcaaa aaaatttaaa tttatatcta tattatttaa
     6361 caaatttcaa gctatattca tatcttgaaa tataagggaa ctaaattcat ttaaagaaag
     6421 ggtgatttga tgcaagatgg tttcttaaca gtaagcataa ttgatgctac taacaataga
     6481 cctattcaaa atgcagtagt aaacatatat tcaatgtcta atggtagcca aagctcatca
     6541 actttatacc aaaacctaag aagtaatgaa tctggtcaag tgactggttt agtattacca
     6601 gcaccagatg tagattactc tctacagcca tctgatgtta gaccttatag ccagtatata
     6661 gtggaagcca tagcagatgg atatgagaca gttgtcatag aaggaacaca gttgttagct
     6721 acaattgaag caagacaagg agttcccatg tctcctagaa caagaagtaa gcgttctttc
     6781 tcaagacaaa gtgagttgat ttttgacata ggagaacata ctctatatgg tacttatcct
     6841 cctaagattc cagaaagtaa cttaaaacca ttgccacctc ctactggttt cgttgtatta
     6901 gataaccctg tagtgcctga atttatagta gttcacgatg gtttgccaga agattcatca
     6961 gctccaaact attggattcc atttaaagag tacattaaaa acatagcatc atctgaaata
     7021 tattcaactt ggcctgaaca gacaatctat gcaaatgtaa tagctataat atcatttact
     7081 ttaaacagag tctttactga atggtataga aataaaggat ataactttac gataacttca
     7141 acaacagcat acgaccataa gtttataaat aatagaaact tatttgaacc aattaacgta
     7201 gttgtagatg ctatttttaa tacttttata aaaagacctc ctacatcaag acagccacta
     7261 ttagcacaat attgtgatgg acaaaaatcc caatgtcctg accaaatgac tcaatggggc
     7321 agtaaagact taggtgacca aggatatgac tatgagagta tactaagata tttctatgga
     7381 gatgagatag tatttgaaag agcgcctata gtaagtggtg taccagtgtc attccccggt
     7441 actacactac aagttggttc ttctgggcag tatgttagaa ccatacaaaa tcagttaaat
     7501 gctatatcta attcttatcc agctgtacca aaagttatag aagatggtat ctatggaaca
     7561 gatacagaaa acgctgttaa gatattccaa ggaatctttg gtttacctca aagcggagta
     7621 gtagatttta aaacatggta tgaaatatcc agagtttatg tagctacaac tagaatagct
     7681 tctttaaatc ctttaattta aagcttgatt tagaacataa actcaaatta ttgaataaaa
     7741 ttattaattt aaaataaaaa tggctgttat gaactaatat ataccataag tatttttact
     7801 tataaggatt gtagtcttaa cagccatttt ttagttataa cttgtttttt ttagaatctt
     7861 aaaactttaa atgggggatt ttaagcttct taaaaacagt gcttagcaac ttagatttct
     7921 ttaaaatatt atatttattt ttaagtttga taaaatgcta ttctaacgaa aagcttactt
     7981 tttatttaaa atctcactta ttatttaaaa tgctaattct cacttagaat attactcctt
     8041 attatttaaa acactatcta tcacttagaa tattacttct tatctaaatt attatttatt
     8101 ataaatataa acaatattaa tttaaaaagt tattcctaat ttaaataata ctattatctt
     8161 catcattaaa cctaaacggc aactttagac ttattagcta aagccaatat tagacctaaa
     8221 gctatagatg caaatgcaga agcaattact ctatactcag cagggaataa gaatacaatt
     8281 gtatgacatg gtatccagaa aaatggtata gtttttaaaa cactaaaacc aaggtaagca
     8341 ttccagtcca caatatttac cacatcttta agttttacct taatcccctt tccaacctca
     8401 tattttttat ctataaaaca gtccataacc ttatggaata aaaatacagt tggtgacatt
     8461 aggatattca tagttgcact cttaaataga gcatttgcaa aagctattcc ctcaaatgga
     8521 agcataccat ccacttgtgc ttgacctaca cctgatgagt agattgacat tccatatgtg
     8581 gtaactacac ctattattcc ccaaactatt gctctataaa atactcctac tggtattttc
     8641 caatccttat acaaaattct agcaccaagt aaatctccca ttgtagctaa tacagcaaac
     8701 tttaaaaaac ctccaatata tggatgtgca cctgttgctt ccataaatac ttctctacta
     8761 cttggtattg ctaagacggc aataatacaa ataagaactc cactccatat aaaatcgcct
     8821 tttttcatat ttataaatac tatttaatat ataaatagtt ctcctttcta ttaaagattt
     8881 atttaattta caatctatct acaattattt attaatcact tattattaga tattcatttt
     8941 ccatatttaa atttattcta tagctttatc tatttttttc tgatattctt atccaccatt
     9001 atctatatat ttattttacg attactcttt cactctaacc ctttaattat ctattttttc
     9061 ttactattct tatcaaccat catccatata tttattttac gattactctt tcactctaac
     9121 tctttaatta tctatttttt tcttactatt cttatcaacc atcacccata tatttatttt
     9181 acgattactc tttcacccta accctttaat tatctatttt tttctgatat tcttatctac
     9241 cattgtctat atatttatct tatgattact ctttcaccct aaccctttaa ctatctattt
     9301 tttctgatgt tcttatctac tattatctgt atatttattt tatgattact cttttaacct
     9361 aacctcctat actttataat aatatgtttt taaccttttt cattattccc aatatgcttt
     9421 ataactacaa atgctctccc cccttaaaaa taataataaa attaatcaac aaatttataa
     9481 tcatattatt actttatgta ttaagcaatt actatgccaa taaattttga tttttttaaa
     9541 caaatcttaa aaataccaaa atgctaattt tctttaactt ttaatgaatc taatcgataa
     9601 catttaattt ttgttatatt tttcagaatg tttcaattat tatttttaag aaaaaaaaat
     9661 aatgttaaca tttcttaaca tacattaaaa aatgttaaca ttataaattg ggtttaatac
     9721 ttttactttg actcgattta cttttattct acttcttact ttatttttaa cttagacttt
     9781 atttttaact taaactttat ttttttatta cttatttgag tcttatttat tttattctca
     9841 ctccatatgt tcaattacat ttgtctaagt tttttgatac ttttattaca taattttaac
     9901 aagcttaagt gttatttggg tagtgcagtt gataattatt taaataatca tcaactgcat
     9961 atttgtatca ttatagtagc ttaatgtatt taatcatact tacttaggat tttgaaatat
    10021 cttatagatt atatttaaca ttagtcaatt gctgcataat tgtaagcaat tgaaatttga
    10081 gacccatctt cattttttat agtgataggc ttcataattt ttaatccttc agtttttatc
    10141 ttttctaatt cttgttccat tttcttaatt gtttcctcaa gatctttttg acttacatct
    10201 ttgactgcat tagggtcttt agcatagtct tttaattctt gaatatgttt agcgtactct
    10261 tcataagtat aatgctcaac accatacttt tccaattcag tagggtcagt tattcctgat
    10321 tttattagag attcaagttt aggtaaagca atattagatg aatcatttcc attcgcaaat
    10381 actgctccag ttcctcctag taccattgca gataccatca ttgcacttat aagggatttt
    10441 tttattttca taaccaatct tcctttctta gagatttatt tgggtagtgt agtttaaaat
    10501 gactattgca atagttatta actacactct tagactatca tagtagttat atttatgtca
    10561 agccttttta aaaaactttt caataaaaaa tactatctca aaacaaaatt aattattttg
    10621 agatagtatt ttaattctaa atcttttata ttactaagat ttatggttac tctatccatg
    10681 ttctatattg ttccttatta actgctaaca aattcccttt tcttgatatg acaaagcgat
    10741 tatcattata gttacttcgc tcaattgaaa cttctcctga atcagtataa agtacatcct
    10801 tactattact agatttattt acatacgttt cttcactatt ctcagaatta gttactttgc
    10861 tcgtagatga ttcttcatgc tctctaaatt caattacaga tacttttgcc gtactaaaat
    10921 gagtctctcc atcactttta gctttactaa caagaatatc agattctaat tttggagaat
    10981 ataaatcagt tgatttacta cttattgaaa atccaatttt atcactttgt tcattttttg
    11041 ttaaaccaaa agattcaacc tcattaatct ctttattttc tgttgcaaaa acaagacctg
    11101 taccacaaac taatacagtt gaaactaaaa aagtaacata agttgatttt ttaaatttca
    11161 taatattaac tattctcttt tctatttttg tctttccaaa cccactacat attgtcattg
    11221 aaaatggatt attctcctct gctagactta ttagtgtggt tgcgtaatca gccctatatt
    11281 cttcaccaag tatactcata accttttcat cacaactaaa ttctatgtct ttatttagta
    11341 aaaaaaacat cacccaaact aaaggattaa accaatggac aacaagagct acaagcataa
    11401 ataactttaa cagattatca aagctattaa tatgaaccat ttcatgagtt aaaacaaagt
    11461 ctaaagtttg attattagca taattcattt gctttggtaa aataattcgt ggttttaaaa
    11521 taccagatgt aagtggtgtc ctagtaaatt cagatatgca aacaataaat cttctaaaac
    11581 ggctattctt acacatccac tttttaatga actcatgatt ttttatatga attgaagttt
    11641 taaaaagatt aattatttta aaatatctaa agcataaaag tatgctaaac ataatagtaa
    11701 ctataaaaga caaaatgccc aacatttcca acatcataat attttcaata ctagacatta
    11761 tttcaaattc gtcagaattg tttttacctg cagagtcact agctttctta agagtataat
    11821 tttctgttga tgtattaccc aattctacat caaaagtcga ttcaattata ctataaatac
    11881 taaaaccaga atttaaactt actggtatta gaagtttaaa taaaacaatc aaccacaata
    11941 atacaaatac ctttttaggt aattttttaa ataaacatgc tcttataaaa ataactatta
    12001 aaagtaatac tccagaagta aaactcattt ctaacaattt aatgaacata aatcttacct
    12061 cactttaatt tttcaacaat ttgtttaagt tttgtaatct catcatttga aagattttct
    12121 ttattaataa aagctgaaag aaatttttct gaagaacctc caaacatttt attaattaaa
    12181 gtttctgttt cctgtttttg tacttcctct ttgcttatta aagctttaca aacaaaattc
    12241 ggctcaattc tctctattgc accttttgac actaattttt ttataacggt atacgtagtg
    12301 tttctattcc atcctgtttc ttcttttaaa atttttacta actctccagc cttgctttct
    12361 cctctctccc atagaacttc cattacttta agttctgaat caaataattt tatctccata
    12421 taaaatcgct cctcaaatat agttatcaca atagtcatta ctataataaa aaactatcat
    12481 agtagtctat actatgtcaa tcatttattt tttaataata aaaactgtct tttaaaaaaa
    12541 ttcttataaa agacagtttt atcaatgtta tactttaatg tttaattaaa gccatatttt
    12601 atttaaatgt ataaccatct atcttagtac atagtataag atttttcttt atctaatagt
    12661 atatcctcta catctggatt ttcttttact aattgtctat aaaaattaca attagtaaca
    12721 cctacatatg gaattaacca tagtagacct attccaagag tagctattga aagtatatac
    12781 catcctataa aactaaagtt tagtataaat aatttaagta catttccttt catcatatct
    12841 aaactagcac ccattgattt aaatacaccc atattatcct tgtcttcaca aattaagaaa
    12901 gtagcttgag aaacatataa tagcaatata taaactggta acataagaac aagtattata
    12961 agaactagag ttattccagt agaaataata gtccctattc caacattatc acttgataaa
    13021 tattcaattg ctccactagt aagtaaaact ccaaatgata aaaacgctcc tataattgtg
    13081 attataaaag aataaatata aacaataatt gattcaaata aataaaaacc aaaacttctt
    13141 aaataaattc tcccacttac aaagatatct cctatcttga ctttttcatc tttagtaatt
    13201 ttcaagaaca tacatgtgaa tcctgctgaa acgcaaaaag ctattacagc cactacaata
    13261 gtagtcaatg gctcaaaagg taagcctaca attgatatta acactaacaa tgcaatatat
    13321 actgccatag aaattgcaca tattccccag tttccagata actgagcttt agatactctt
    13381 tttaattctt gccttgatac cataaaaaac acccctttca ttttataaaa aacttttaaa
    13441 aatataaata accttttaac gtataatatt aatatatcat attttcgact atataagata
    13501 caattattca ccatgtttta tcaagatatg ccataattgt attactagtt ttatatagtc
    13561 aacattacca aatacataaa ataa
//
LOCUS       DAEMAE010000094        13520 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEMAE010000094 DAEMAE010000000
VERSION     DAEMAE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.096"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            952..2310
                     /locus_tag="KPH93_002137"
     CDS             952..2310
                     /locus_tag="KPH93_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG1688955.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIKLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPKNKSDKIEDKNK
                     SL"
     gene            2418..3212
                     /locus_tag="KPH93_002138"
     CDS             2418..3212
                     /locus_tag="KPH93_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG1688956.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKCGFSDLIPALLVVGAKVRLYNKGMLELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLAFEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYNG"
     gene            3205..5511
                     /locus_tag="KPH93_002139"
     CDS             3205..5511
                     /locus_tag="KPH93_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1688957.1"
                     /translation="MVNKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDIKVGADKEGNLKVIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFDARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELKQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKEILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFSSDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPKFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRIRELPMTFEKVYMGMKNNNKEL"
     gene            5650..6927
                     /locus_tag="KPH93_002140"
     CDS             5650..6927
                     /locus_tag="KPH93_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1688958.1"
                     /translation="MPNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGTLNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIVINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7110..7832
                     /gene="yqeC"
                     /locus_tag="KPH93_002141"
     CDS             7110..7832
                     /gene="yqeC"
                     /locus_tag="KPH93_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG1688959.1"
                     /translation="MELSNIIKKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDEFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTNSINSSSTVSIENLKNIVLNPNGLFKHYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCARY"
     gene            7939..8508
                     /gene="mocA"
                     /locus_tag="KPH93_002142"
     CDS             7939..8508
                     /gene="mocA"
                     /locus_tag="KPH93_002142"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG1688960.1"
                     /translation="MINAVIMASGFARRMGANKLLLDYKGCSIIEHVFRAVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYINKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKVDYERIGGKL"
     gene            8505..9524
                     /locus_tag="KPH93_002143"
     CDS             8505..9524
                     /locus_tag="KPH93_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG1688961.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLIIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9860..11485
                     /locus_tag="KPH93_002144"
     CDS             9860..11485
                     /locus_tag="KPH93_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1688962.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11684..12133
                     /locus_tag="KPH93_002145"
     CDS             11684..12133
                     /locus_tag="KPH93_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688963.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGYNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYISTKENSGVTVSSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSLTEAKSDNSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12341..13453)
                     /locus_tag="KPH93_002146"
     CDS             complement(12341..13453)
                     /locus_tag="KPH93_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1688964.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYKDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMFLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         5273 a         1439 c         2431 g         4377 t
ORIGIN      
        1 ttgtatattt tatgttgcag atacaaatat agtaaaggag agtacattgg tacaactgag
       61 atacatagct tattaagttt ttctgtctta tgagaattat attataaaat attcataaat
      121 attttgagtt taaattaaaa aattatttta tgcttagatt aattgatatt taattaattt
      181 aataacaaaa acagtgaaag agtaaataat attatttaca gggggtaaag caaagtatgt
      241 taaataaatt gtacttttag ccccgtacat agagaaatct atgtaggaca caactttaaa
      301 accagtaaat cctaaagcct tgcaactaca ataaaagata gaaagaaact tttatgaatg
      361 tggtgaaaac cagaaacaat agtgaggatg gtatatggtg agagcctaag tacttaaaaa
      421 tggacatttg gtcgccaagc ctagaaatag gaaggtcaaa cgactatctc gagagagagt
      481 acactataag cgattgatag tggaaacggg ttgctcttag caggtaatgc tgaagatgaa
      541 gaaatagtct acgctcatat gaaagtatga ggtcactgga tttgaagtga catctataaa
      601 gtagcgttta taggtaaatg tttaaaactt gaaacatgta aatacttatg aaaatttata
      661 acttataatg attaagattg aaagatttaa attgaattaa atttactata aaaaattaaa
      721 aattcatagc tataatactc ttatatttgt aaagcattgt atataatgta tacaaaatta
      781 aagaataggt ttatttgtta ataattaaat ttattgcatc tgttttttaa gactaaaaac
      841 ttaaacatat gttggagtat agttgatatt tatgaacatc attaatttaa tatattagat
      901 ttttaagtat ttatataatt caataagttt tatgtaacgg gagtttttat tatgaaagca
      961 aaaagtacat caaaatttga attagacgga ataccaccac taaaggaagc aataccttta
     1021 gggttacaac acttaatggc aatggtaata ggtagttgtt taccagcgat attgattgca
     1081 aatgcagctg gacttaatca taagatgtca acattattga ttcaagccgc attagttttt
     1141 gcaggactat caactttatt acaattatat ccaatacctt tgtttaaagg ttttaaatta
     1201 ggaggaggaa taccagttat aatgggagct acagctatgt ttataggagc tggggtatct
     1261 gttgcaagta agtatggact accagtatta tttggctctc aaataatagc tggtatagta
     1321 ataattataa ttggttttgg tcttaaaaaa atacgattta tatttactcc agctgtatca
     1381 ggaacaatag ttgcatgtat gggtcttgga ctttttccaa tagctataaa aaacttagct
     1441 gggggtatgg gcaatgaaac ttttgggaat ccaattaatt ttatagttgg aatatctgta
     1501 tctcttatga tattaggatt aaataagtat ggaaaaggat tatttaaaga tgcatcaata
     1561 ttagttagca taatttttgg atacatttta tcattaattt tgggtatagt aaatttttct
     1621 tcaattcaag agtttacttt ggttgcactt ccaaaaccac ttgcttttgg tctagatata
     1681 aagcttgaag ttgttgtaat gttttcaata atatatttag ttgagatagc tgatataatg
     1741 ggtgcttgta cactttctgc ggtaggaggg cttaatagac aagtaacaga tgaagaactt
     1801 tcttctgctg ttttaggaaa cggagtactc tctacaatag gtgcattatt ttctgcaact
     1861 ccaatgggga tgtttggaca aaattctgca atcgtttcca atacaaaggt tgtaagtaaa
     1921 tttgtactag ctattggagg aattggacta ttcttggcag gaatatcacc tctattagct
     1981 aatttaataa gaacaattcc tccctgtgtg gttggaggtg caacactcgt tatattttca
     2041 actcttacaa catctggttt aagacttgtt agtatggatg gatttaatca agaaaatagc
     2101 atgatattag gtctttctat ggcttctggt attggattca tggtagctcc tcaagttctt
     2161 gaaaaatttc caaagtttat agaaacatta cttgcagatt caagtgtagt ttcaggagca
     2221 atggtagcta taataattca agctttatat atggtaaaat ttcctaaaaa taaaagtgat
     2281 aaaattgagg ataaaaataa aagtttataa tgtaaaggtt taatatatgt gaattaaata
     2341 tactacaatt ataaatataa ataaaaagat ttattaaaaa cttttgtgtt ttaataagtt
     2401 aatgatgggg ggataagatg gttactataa aggaatattc agttccacag agtttagaag
     2461 aagcatataa aattttaatt tctaaaaaaa ataatgttat tttaggtgga tgtggattta
     2521 taaaattaag taataaaaat ataggaacag cgatagattt aaaagatata aatctaaatt
     2581 atataaaaga ggatgaaaaa aatattttga taggtgcaga tacttcactt agaagtttag
     2641 aaattgataa aactataaaa aactactgca gtggaatact ttcaagcgct gtttctaata
     2701 tagttggtgt tcaatttaga caaggggcta gaattggtgc aagtgtattt tcaaagtgtg
     2761 gcttttctga cttaatacca gcacttttgg ttgttggagc gaaggtaaga ttatacaata
     2821 aaggaatgct agagttaagt gagtttctgg atagtgaatt aagtagggat atattggtcg
     2881 aagtaatact accaaaaaaa gatgcgatag ctgtttttga ctcaataaga aagtgtacag
     2941 gagatttttc tgtattaaat tcagcaatgc taaaagaaaa tgatacttat aaaatagcaa
     3001 ttggggcaag acctcaaaaa gctagattag catttgaggc aagtaatata ttaaataaag
     3061 aaaaagatat agataaggca tctattgttg ctagtaagga acttacttat ggaagtaata
     3121 tgagagctag taaagagtac agaaaggata tggcaagtgc attagttaaa aggatgtata
     3181 atgctattga ggagggaatg tataatggtt aataagggga taagaaagat agatagtaca
     3241 gcaattgtca caggaaaacc tctatatact gaagacctca ttatgcataa agatgttttg
     3301 acattaaaac tacttagaag tccacatgct catgctaaga taaagaatat agatacatca
     3361 aaagccgaaa aaattccagg agttgtgggt atttatacgt ataaagatgt accacaggtt
     3421 agatattgtg ttccaggtca aacagagcca gaagcatcac cttatgatag agttttactt
     3481 gaagatacag ttagatttat tggagatgaa gtagctttag tggctgctga agatgagaaa
     3541 acagcactta aagccttaaa aatgataaag gtagactatg aggttttaaa gcctgtgttt
     3601 gatgcaagag aagcagaagg aaatgaagtt gttatacatc cagaaaaaga catagtagaa
     3661 ccataccctt ttggattgga cgcaaaaaat aatatagtat caaaagaatt ttttgaattt
     3721 ggagatgttg aagctaagct taaagaaagt gattttgtgg ttgaatcttc atacaaaacc
     3781 caaagtcaat cgcattgcat gatggaaact cataggtcat attcatataa agatataaat
     3841 gatagaattg ttgtagtttc atcactacaa tcaccacaca atgtaaagag aatacttaga
     3901 aaagtactgg gtataccatc ttcaaaaata agagtaatta aacctagaat aggtggtgga
     3961 tttggaggaa agaatatagc agcaacagat atatttactg catttgttac tatgaaaact
     4021 ggtagaccag caaaatttgt atttacaaga agtgaaactt ttgtaggttc taatagcaga
     4081 catgaaatgt tttttgatat taaagttggt gcagataaag aaggaaattt aaaggtaata
     4141 gagttaaagt cattaaacaa tacaggtgct tatggaggga atggtgtatc tgtttcttcc
     4201 gaatctggac acaacacctt accaatatat aatgatattg atgctgttag atttgatgct
     4261 agaacagtat atactaatag gttaccagca ggagcactta gaggatatgg tgctacccag
     4321 ggaatatttg caatagatag tgctattgac gaattggcac ataagattgg aatggaccca
     4381 cttgaattta gaattaaaaa tataatagat agatatacta acggaaaagc agctgaagac
     4441 gaaatattaa gttgtgagtt aaaacaatgt atagaaaagg gtaagaaatt aattaattgg
     4501 gacgataaat atccatgtaa agatatagga aataacaaag taagagcagt aggtatggca
     4561 gtggcaaatc atggttctgg tataccacgt gtagatatgg caacagtgac tataagaatg
     4621 gatgaagatg gcacatacaa gctattatct ggtcttgctg atttaggtca aggagctgat
     4681 acaattcaag cacaaattgc tgctgaagct ttaaacacta ctgtagatag aatatctatt
     4741 tatacaggtg attcagattt atgtccatat gatacaggag catttgcttc ttgtacaaca
     4801 acggttacag gaaatgcaac aataaagaca gcaaataaat taaaagagat attactttca
     4861 gttgcagagt caaagctagg atttaaaaaa gaagatttag agttgggaaa agatagagtt
     4921 tttgttaaaa acgataatag taagtttgtt ctattggaag aattggggag agaatctgtt
     4981 ggaggaatag gattttctag tgatgatgtg ccagtagttt tggaagcaac agaatctttt
     5041 ggcatgttaa aaaaaataaa accatttata gcaggatttg cagagataga attagataaa
     5101 aatacaggcg aatataaggt aataaatttt gcatgtgtac ctgattgtgg aacagttata
     5161 aacccaactt tggctagaat acaagtagag ggtggtgtga tgatgggtat agggtttgca
     5221 atgtatgagg accctaaatt tgataaagat ggaaggttac taacagatag tttcttacag
     5281 tataaagttc ctacaaaaaa agaagtggga aatatattgg tagatttcgc tgagaatttt
     5341 gacccaggag gtccttatgg tgctaaatca ttaggagaaa tagtcataca tacaccagca
     5401 ccagctatag caaatgcaat atttaatgct actggagtcc gtataagaga gcttcctatg
     5461 acttttgaaa aagtttatat gggtatgaaa aacaataata aagaattata ataagtaata
     5521 tggtatataa aaacatgtaa atttaaatta ttttgtaaaa taaagtaatt aaatggacat
     5581 tatatatggt gttttgtaaa aataccgtat ataatgtcta gtagagtata ttctaaagga
     5641 ggtgagttca tgccaaacag aataaaaaat atacactatg gaactcagag acaacaatta
     5701 aaaaacttaa ctaaaaaaga atatcaagct ctacgagaga tgtgcttctt agctaagaac
     5761 ttatataatg tcggtacata taatgtcaga cagcattatt ttaatcaagg agaacatctt
     5821 aactatgaag ctaattatca tgtatgtaaa gaaaatgaga attataagct tctcaatagt
     5881 aatatagcac agcaaatatt aaaagaagtt gacagttcgt ttaggtcatt ttttgggtta
     5941 attaaattag caaaagaagg aaaatatgat tttagaggga taaagtcacc taaatatttg
     6001 gataaagaag gtttttttag tataataata ggtcaaataa gaatcaaaga agatgggact
     6061 ctgaatgtac caatgtctcc agcatttaaa aagctatatg ggaaagtaag tataaaagtt
     6121 ccacaaaaca tactagagaa aaaaattaaa gaaataagaa taatacctaa acataaagct
     6181 aggttctttg aaattcagta ttgttatgaa ataccgaaat cagaggaagt ttcaaatcaa
     6241 aaaaatgcac tagctataga cttaggttta gaaaatttat gtacatgtgt taccaattta
     6301 ggtgatagtt ttatcataga tggaaaaagg ctcaaatctg tgaatcagtg ggcaaacaaa
     6361 cagaatgata aaatagaagc cttaaaatta aaaaagaata taactgcaat aacaagaaaa
     6421 caatttaaag tttggaataa gagaaataat caagttaaag actatgtaaa taaaacctgt
     6481 ttttatataa ttgattattg tgtaaaaaat aatattggaa atttaattgt aggatataat
     6541 gctaaattag aaaaaagtga taatatggag agaaaaatat atagaaattt tataagaatc
     6601 ccatttggag agataaaagg aaaattagaa tatctgtgta aaataaaaaa tatcaatttt
     6661 attaggcaag aagaaagcta tactagtaaa tctgattttt ttgctgatga ttttctacct
     6721 gatatcaatg tggacaatgt taaaaagtat gactttaaag gggaaagaat aacacgtgga
     6781 cagtacaaat ccagtgttgg aatagttata aatgcagatg taaatgcgag tcttaatata
     6841 ttaaaaaaat cacaagtagt taatttatct aatttacaga ataatcctca aatattaaag
     6901 caaccacaga gagtaagaat atcttaatta tatttaaaag ataaactgat agcgtaagct
     6961 tacagcttag cggcgaattg gatccgcctt gttgtttaca acagaatcta tttactttcg
     7021 agtttatgga atttcaaaaa tcacttttac taaaaaggtc tagctataat aattaagaga
     7081 ttagaaaact ggttataaga ggtgaaagtg tggaactatc aaatataata aaaaagaatg
     7141 aaataataac tgtagttgga gcaggaggaa aaacttcgtt tataaactat tttgcaaatt
     7201 tttatagaga taaattgaaa gttctcctta caacgacaac taaaatatat gtaccaaacg
     7261 attatgacaa tataattatt acaatagatg gaactgttat tccatctatt tgtcatggaa
     7321 taacagtatg tggcagttat ataaataatg aaaataaatt agtaagtatt gattcctcta
     7381 ttttggatga aattgtagac gaatttgatt tagttttaat agagggagat ggctcaaaga
     7441 gaaaaaaact taaaggttgg aatgctaaag aaccagtagt atatcataaa actacaaaaa
     7501 ccataggtat attagatata acatcatttg gaatgaatat taatgaagag aatatacata
     7561 gagtagaaat atttaaaaaa atagcaaatt tagatacaaa tagtattaat agtagctcta
     7621 cagtaagtat tgaaaaccta aaaaatatag tattaaatcc aaatggatta tttaaacatt
     7681 attctggaaa aagagtatta tttattaata aagttgaaaa tgaaaaatat aaaaatttag
     7741 ctataaaatt aatagaaaat attaaagaat atgaaagtga aatagagata ttttatggaa
     7801 gtgtaaaaca aaaattttgt gcaagatatt gaagtattaa aatagtttat atgatattat
     7861 atatatagat aatatcaata gagatattat aaaaatcata gaactatcaa tatattataa
     7921 ataccaagga tgatatatat gataaatgca gtgataatgg catcaggttt tgccagaaga
     7981 atgggagcaa ataaactttt attagattat aaaggctgtt ctataataga acatgtattt
     8041 agagcagtta gcaagattga ttttcatcaa gttgtagtag taagtcaata tgaagaggtt
     8101 ttgttattgt gtaataaata tggttttgaa tatatagata ataaaaatgc aaatataggt
     8161 caaagtgaat ctataaaatt gggagtttta aatagctcaa aatgtgatgg ttatatgttc
     8221 tttgtaggag accagccatt tattgataac ttatatataa ataaaattat aaatactttt
     8281 aaattagata gagattttat agtaattcca agatacaaag atatgtgtgg aaatccagtc
     8341 atattcccat atagcaagaa agaagaattg ttggcactta aagaagatga aaagggtaaa
     8401 accattttaa aaaattcatc aaaaataaag tacgtagaag ttcctaaaga aatgttgttt
     8461 gatatcgata caaaagtgga ctatgaaaga attgggggta aattatgaag ctaataaaaa
     8521 ctatagatgc tgttggacaa gttttatgtc acgacataac tcaaataata cctggagaat
     8581 ttaaaggaag aaaatttaaa aagggtcaca tagtaaaaga agaagatatt ccagtgttat
     8641 tatctcttgg taaagataat ttgtatgtat gggaaaagtc agaaggaatg gttcatgaaa
     8701 atgaaggtgc attgttttta aaagaactaa ctgctggaga aaatttagat tttagtgaaa
     8761 taaaagaagg aaaaatagat tttatagcag catgtgatgg tctattaaag atagatgttg
     8821 atgcactttt tgatttgaat tgtgttggag aaataatgat ggctactctt cataataatt
     8881 ttatagtaaa taaaggttta aaagtagctg gaacaagagt gatacctttg attatagatg
     8941 agaaaaaatt agaagatgca aaaaaagtag ttggtaatag aaaaatagta aatgtagtgc
     9001 cttttaaacc taagaaagta ggaattgtta ctacaggaaa tgaagtattt tatagcagaa
     9061 ttgttgataa atttggacca gtaatagaag aaaaagtaaa gggctttggt tgtgaggtta
     9121 taggacagac tatatgccca gatgataaag aaattataaa gagtgcaata aaagaattta
     9181 taagtcaagg agcagaactt atctgctgta ctggtgggat gagtgtagat ccagatgatg
     9241 ttacgccaac agctataaaa gaaactggag ctgatttagt gacatatggt tcaccaatat
     9301 taccaggggc tatgttttta cttgcttatt atggagaagt tccaattatg ggtataccag
     9361 gatgcgcaat gtatcacaaa acaacagtat ttgatatagt tttatctagg gttttaattg
     9421 atgaaaaact tgataagtat gatatagcta gatatggtca tggtggcctt tgtatgaact
     9481 gtgatgtatg tacttatcct gcatgtaatt ttgctaaaat ataaagttat taaaaaagag
     9541 tatttacaaa ttaagtactc tttttaaata tgaaacaaac agctagaatg atgttttaag
     9601 aaaagcatat tatttagaag ttgtaatagc ttattttcaa tgtgtaaaag aatgaataaa
     9661 gttgcaattt gatttcttta taagtataat ataagagatt gttaattttt ataaatttac
     9721 aatttttagt attattttaa taaatatttc acaaatttag tataaaaaat atactattta
     9781 cgtgataata tataataaaa atgttatcaa agttttatga taaaataaat tatgagcaaa
     9841 aagaaaggaa aatatattaa tgttacaagt tacaggtgtt ggacttagat ttggtgataa
     9901 ggaactattt aaagatgtca acttaaaatt cactaaaggt aattgctatg gaataatagg
     9961 agctaatggt gcgggaaaat ctactttttt aaaaatatta tcaggagaaa tagagccgaa
    10021 tacaggtagt atatctataa ctgataaaga aagaatgtca gttttaaaac aggaccactt
    10081 tgaatatgaa gaagaaactg ttttaaatgt tgttataaga ggtcatgaga gactttggaa
    10141 cataatgaaa gaaaaagatg cattgtatat gaaggaagac tttaatgaag aagatgggat
    10201 aaaagctgcc gaacttgaag gtgagtttgc agaacttgac ggatgggatg cagaaactaa
    10261 tgctgagaaa atacttatgg gtcttggcat aacgaaagat atgcattata aacaaatgaa
    10321 agaactagtt ggtggggaaa aggttaaggt attgttagca caatcacttt ttggtaagcc
    10381 tgagatacta ttaatggacg aacctacaaa ccacttagat tttaaatcaa taaactggtt
    10441 aaataatttt ataatggatt tagaagagtc tatagttata atagtatcac atgatagaca
    10501 tttcctaaat caaatatgta ctaatatagt tgacgtagat tttggtaaaa tacaaatgta
    10561 tgttggaaac tatgatttct ggtatgagtc aagtcaattg gctttacaac tggctaagga
    10621 ccaaaataaa aagactgaag aaaagatagc tcaattaaag gaatttatag ctagatttag
    10681 ttctaatgct tcaaaggcta aacaagcaac atcaagaaag aaacagttag agaaattaga
    10741 agttgaagat atacaaccat caagaagaag atatccatat gtaggattta cacctgcaag
    10801 agaaattggg aatgaagttt tagaagtaca caatttaact aaaactatag atggggttaa
    10861 ggttcttgat aatgtgtcat ttagattgga tagagatgat aaagtagtat ttatgggtga
    10921 tgaaatagct actactgcac ttttcaacat agttatggga gagcttgagc cagacagtgg
    10981 agagtataaa tggggagtaa ctacttctca agattatctt ccaaaaaatc ataataagtt
    11041 ttttgacgga gtagaatatt ctttggttga ctggcttaga cagttctctg aagaaaaaag
    11101 tgaaagtttt ataagaggat ttttaggtag aatgttattc tctggtgaag aagcattaaa
    11161 agaagctcaa gttatatctg gaggagaaaa ggttagatgt atgcttagta agctaatgct
    11221 ttcaaatgca aatgtacttg tattagatga ccctactaac catctagacc tagaaagtat
    11281 aacttctgta aataaaggac ttgaaaagtt cccaggagtg cttttattta cttctcatga
    11341 ccatgaattt atatctacta tagcaaatag aattatagag ataactccaa atggaataat
    11401 ggatagaaaa atggactttg atgagtattt agaaagtaaa gagatacaag accaattagc
    11461 taaaatgtat ggaaaagata aataattatt agtataaaac cttttaatat ttgagacatg
    11521 ctatgcataa gtgtgtaagt ttttaatcaa atattaggag gttttttatg ttgtttaaat
    11581 atatttatta gtagaataga gtaatatttt gtatattaag aatttaaata taatatagta
    11641 gatgatataa taaaaatata taacattttg gggggaatag attatggtta agaaaaggtt
    11701 agtagctgta gtactaagtt tagctatgat tagtgtaggg aatttttcat atgctcactc
    11761 aggagggtat aattcatcag ggggtcatca taacaaaaat aaatcagagt taacgcgtta
    11821 ttattgtgga ggacattcag cacatcaaca caataaaggt ttttgtcctt atattagtac
    11881 aaaagaaaat tctggagtaa cagtatcttc agtacataac gaaaaaaatc ataaaaatta
    11941 ttttgcagaa aaagggtata ataaaggtta tgaagatggg tgtaaagggc tttataattt
    12001 aagtctcaca gaagccaaat ctgataattc atatgaaaaa ggatatttaa ttggatacaa
    12061 tgaatataaa gcaaaaaaag tagatgatgt aatgaagaag atgttagata ttccaatgct
    12121 agcaagtata taataagata gtatttaaaa agatattttc tataaggaaa tatctttttt
    12181 attgtaaagt ataatttttc ataaaaaagt atactaactg aggtgataaa ttaaaataaa
    12241 acagattatt taagtagtta ctaatttgag atagatacat ttaatattaa tgaatctatc
    12301 tcaataagct aagctaattt aaagctaaga tagtttaata ttaattagca ttaaataaac
    12361 tttcagaaat tttgtcagaa gcttctttat ccatttcttt aagtacatga gaataaacgc
    12421 ttagagtagt acttatattt gagtgtccta ctctctctga tataaccttt ataggtactt
    12481 tagaatttat aagtaaagta acatgtgagt gtcttaaatc atggaatcta atatgaggca
    12541 attcattctt ctctaaaaat cttttgaatt ttttactcat aacatcttca gcaataggtt
    12601 cacatttctt attaaaaaac aacaaattat tattatttct aatggaagat tttaataaat
    12661 ttttattctg ttttatttta tattctttta ataaaaacat taattctttg ggagaagata
    12721 tttttctaac agagctttct gtctttggct ctttgagaat aacagaacca ttaattcggg
    12781 aagttatctt atttactgtt attgtatttt cttcaaaatc aatattatcc caagttagac
    12841 ctaaaacttc tgaaattcta agacctaatc caatagccaa attaataggt aattccattt
    12901 ctgtattttt tgctaaatct aataatttta tcatttgttc tctatcataa atctcatttt
    12961 taaatttttt atatcttgga gcctctatac tatccataac attttctttt attaatttga
    13021 gtctataagc tctttttatt gcaagtttca atatatttat atgaatctta attgattgag
    13081 gatttagttt ataagatagt ttatctatgt agttttgtat gtgaaccgga tgtaagtctt
    13141 gtattttata ttttccaata gatggattaa tgtaattctt acaaatacta acataattat
    13201 tatatgtagc taaagatata ttttcactat atttatttag aaaatctaat agaaattctg
    13261 aaactgtaat ttcgcttggt actaaaaaac catccttata taagctttct tttacctcat
    13321 ttagtctttt acttgcttcc ctttttttat caaaagtacc catatttttt tgctttcttt
    13381 tacctgtctt ttcatcttca aattccaggt aaacaacata gtttttattt ctttttctaa
    13441 taaatgcatt catatataac ccaccaatca aaattttata catttattat aacatatata
    13501 tcacaaaata attttgaaat
//
LOCUS       DAEMAE010000095        13498 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEMAE010000095 DAEMAE010000000
VERSION     DAEMAE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.097"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(800..940)
                     /locus_tag="KPH93_002147"
     CDS             complement(800..940)
                     /locus_tag="KPH93_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688965.1"
                     /translation="MENLMMSIIAGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(1200..2360)
                     /locus_tag="KPH93_002148"
     CDS             complement(1200..2360)
                     /locus_tag="KPH93_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG1688966.1"
                     /translation="MKLIKTLLAIGMSVSLLIASCPTSNALSSISSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKTDPCNICVK"
     gene            complement(2531..2734)
                     /locus_tag="KPH93_002149"
     CDS             complement(2531..2734)
                     /locus_tag="KPH93_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG1688967.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2821..3855)
                     /locus_tag="KPH93_002150"
     CDS             complement(2821..3855)
                     /locus_tag="KPH93_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG1688968.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGITGAKSNNTYE
                     KEALKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKVKES
                     VYNQGYNEEDFSVPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(3899..4054)
                     /locus_tag="KPH93_002151"
     CDS             complement(3899..4054)
                     /locus_tag="KPH93_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688969.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTYIQNMLKDLFTNTIHQIFTLSNF
                     NGLNIIY"
     gene            4150..4296
                     /locus_tag="KPH93_002152"
     CDS             4150..4296
                     /locus_tag="KPH93_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1688970.1"
                     /translation="MLLENGTNIKDIQHILGHAKISTTMDTYSHVTNNMRNQTVNVFE
                     SIVE"
     gene            4388..4751
                     /locus_tag="KPH93_002153"
                     /pseudo
     CDS             4388..4751
                     /locus_tag="KPH93_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
     gene            4830..4925
                     /locus_tag="KPH93_002154"
                     /pseudo
     CDS             4830..4925
                     /locus_tag="KPH93_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            5183..5371
                     /locus_tag="KPH93_002155"
     CDS             5183..5371
                     /locus_tag="KPH93_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1688971.1"
                     /translation="MDYISVKAASEKWGISERRIQKLCEENRINGTEKFGRAWMIPKD
                     AQKPIDGRYKSTMKVGEI"
     gene            5374..5997
                     /locus_tag="KPH93_002156"
     CDS             5374..5997
                     /locus_tag="KPH93_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1688972.1"
                     /translation="MNFIDIMPKEVKHKLQEKSYAAGNTILYAEEENNFVYFLLSGVA
                     EAYIQSSNGNFSTLYLYKSGSFFGEIEQFYDGKKPVEITALSDCIVKRLYRTYFLEWI
                     KNDFNATTFLIQGLAEKLIINSQLVENILELTVKERLLRSISLHYHRNTLDSLNKSRL
                     AKEVNSPIRSVNRAIDECKKQGLLDFSDNKFIIINPKELLKFLPYIE"
     gene            6113..7039
                     /locus_tag="KPH93_002157"
     CDS             6113..7039
                     /locus_tag="KPH93_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688973.1"
                     /translation="MNVAITRILNILGDVKIVDKIVNLNSSDINSFLLSVFQSAVIKK
                     TPQNILAAFSTNRFTSPSHFDPIAFHNLEVKLLSLSEKLGIKNLLLSPSAPLGSCSVF
                     GCVNQNNVISALRGTETLSDPSNMIAIIIADGLKKKILRNEDAIHHATTTRVVRAQAF
                     EGKGFYAHFGIFCIVSSGKDKGSYSCESELLIKHLNYYKDLFTNIENSKISICLRKRG
                     GYPDRDGFFEKMTAIVKSSLPEIQITIDLNSEDNAYYKGINFKIYLHCNDDVIEIADG
                     GFVNWISEMTGNKKNRCLISGIGIDRLLTLEE"
     gene            7041..7538
                     /locus_tag="KPH93_002158"
     CDS             7041..7538
                     /locus_tag="KPH93_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1688974.1"
                     /translation="MKLENWSIEYINDLVKNANNRNVSKNLRNIFPYPYSVVDAEKFI
                     THCQRCNNKHINLAIIEDDKAIGSVGVSIGEDIYEKSAEIGYWIGESYWGQGIMTHAI
                     KEMIELCFSQYDIVRLYAVVFSHNKASCKVLEKCGFVFEGTLKNSIYKDGNIYDSRIY
                     ALVKK"
     gene            7658..7963
                     /locus_tag="KPH93_002159"
                     /pseudo
     CDS             7658..7963
                     /locus_tag="KPH93_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            8540..8701
                     /locus_tag="KPH93_002160"
     CDS             8540..8701
                     /locus_tag="KPH93_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688975.1"
                     /translation="MENIKNIQGDAIKAPAPNHTFEKTKAYQDFINVLSQIIEKHGAE
                     VLGEINRAN"
     gene            8750..10275
                     /locus_tag="KPH93_002161"
                     /pseudo
     CDS             8750..10275
                     /locus_tag="KPH93_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045075.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10185..11552)
                     /gene="rlmD"
                     /locus_tag="KPH93_002162"
     CDS             complement(10185..11552)
                     /gene="rlmD"
                     /locus_tag="KPH93_002162"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG1688976.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQIGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLEDSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETCVLL
                     SHKNS"
     gene            complement(11579..12097)
                     /locus_tag="KPH93_002163"
                     /pseudo
     CDS             complement(11579..12097)
                     /locus_tag="KPH93_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(12216..13334)
                     /locus_tag="KPH93_002164"
     CDS             complement(12216..13334)
                     /locus_tag="KPH93_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1688977.1"
                     /translation="MVVVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IKNHKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINAGMNILKEGLRLITIQNK"
     gene            complement(13336..13443)
                     /locus_tag="KPH93_002165"
     CDS             complement(13336..13443)
                     /locus_tag="KPH93_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1688978.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQISKYINNQKKQVK"
BASE COUNT         4444 a         2362 c         1892 g         4800 t
ORIGIN      
        1 tctttcatta tggttatata caatatttga attgtcatta aatatgccaa tagttttata
       61 tattattgat tttattaaaa aaaagagata tacaaactaa taagttgtaa tctctcaaat
      121 gtaaaaaacc aataaattag ctcattggat tttttagtcc ataatttcta tagattattc
      181 aacaaacaat ctatagaaat tatggtatta atgtttaaat aaccaacttt ttctaaaata
      241 acctttatag gaatatatga gttcataagt aaagatagcg aaataagaaa ccagtttggt
      301 atgtaaaatc tagattttta attaaactat aaagattata tattatatta tcaaaaatag
      361 aaaattaaaa ttaaattttt tataataaat tgtatacatt ataaattata ttaaattttt
      421 aatttaagta atagtatatg taatctttag tcatcttctt accagttttt tatccacttt
      481 tctaatgaag ttattttttc atatgttaat tcctcttgaa ataattatac taaaagactt
      541 tttataaaat tataacataa aagtttatgt atttttttga tatgaattta tagtacttat
      601 cttctttaat tgagattgta tatatttaac taaatattac atcaataatt ccatgcaatc
      661 aaatcaatta tttccatatt tttttagaat aataataagt atatattcac aaaattttgt
      721 gttaatattt ttaatttgtg atataataaa agcaaggaaa catattttac ttaactttga
      781 gtgatgtttc cttcaacaat tattttgaag atttactttt ctggaacttt atattaagtt
      841 cccagccact cttagttggt cgctcagagt ggctctttaa tttatcgtaa atgtaactgg
      901 ctataacacc agctataata ctcatcatta gattttccat agttttcacc tcctctcata
      961 agaaagtgaa gaaacttcta tggaaacacc actcatagct ttttcaaata tttattcctt
     1021 gctatattca ttatatcata aatattggaa acataaaatt ttaatactta tcgtctttat
     1081 tattattttt tataattaat aattttcttt ttaataatac gtaatataaa tttatatttc
     1141 ttcaataaaa aagacaatct taattaattg tcttttttaa tatatatttg gctaatgtat
     1201 tacttcacac aaatattaca agggtctgtt ttaccttgtg acttagcttg ttttaatgtt
     1261 actttattta ttgttttact tctttttagt gtagaacaat tttttgttag atgatatgat
     1321 tttccattag gagttacata tactgtactt ttatcagtta atccaggatt attagagcta
     1381 ggctcattac ttggtttgtt cgtattattt gaattagtat cttttacaat acctgtattt
     1441 gaatccccaa cattattagt tatatttaga cattgtttta taacaacatc atcaatattt
     1501 ccaatagcag ttattttact tgcatttttt aatatactct tattgcttcc atttccaact
     1561 aacactatag gtgttttttg tgcaattgta gaaccaacta aagagtaaac taaatcagtt
     1621 ccacttgcta tgtaaaattc ttttgagcca ttataaaatt tatttattat ttgtttattt
     1681 gtatcgtatc tatctactcc acctattctt gttgctttag tttcatttat taacttatca
     1741 ctcatactga cttttccacc aattgcataa gtttctaatc catctgtagc aaaacttgta
     1801 gtatttccat cagttaatac tattggagat ttatctctaa ctgctataga agcaacactc
     1861 atagcatctg gttcaccttt aaaagcatta gttaatatta cttttttaac cgtacttact
     1921 cttcctattt cttttgcaac tttatgactt gtatctattc tatcagttcc acttattcta
     1981 tttatactta tttttttatc acttaatatt ttctgaactg atgctcctat cgaatttttt
     2041 cctccgatta gatatacttc ttttaccttt tctaatcttt ttaatgtttc tgaagggatt
     2101 gtatctttct tagttaacaa tattggggca tttttagctc ctgacaatcc acttgctgac
     2161 aatccatcag ataatccatt ttcagaatta ataagtattg caatatcata attttgttta
     2221 tctgctatta aacctgctgt ttcatactta tttgtacctt ttattgaact aatagaacta
     2281 agagcatttg atgttggaca actagcaatc aataatgaaa cagacatccc tattgctaat
     2341 agtgttttga ttaatttcat aatgaaatct tcctataatt tttataatat tacttaaata
     2401 ttataattct acataaaaga atattttcct ttatgaaatt agtttatatt tcaaaatttt
     2461 gtcacttttt aaatctatat actaaaaaat agactattta taacagtcta tttcttagta
     2521 tatcaaatat ttatttaggt ttacgtttct ttgtactagt ttttgtatat gatattccag
     2581 ttccaggtat actcaatgta gctgattttc ttccttttga attaactgac actctagcac
     2641 ctttaacacc agcacttata cctacactct tcttacctaa atttaatttg agtcctcctc
     2701 ctagatttat gcttttccta aagttaaatc ccatagatat aaccttccct tctatatttt
     2761 aaatatttat acttaaaatt atatttgtta tacacttgtc atcattggta tatctagcat
     2821 ctatatcttt ttacgttttt tctttttata tactactcca gcaactacag ctgctgttcc
     2881 agctccaacc ccataagtag catttctact tttctcttct tctctttttt ctaacccttc
     2941 atcatataca ccttttaact tagtttcaat agacttcatt tcatctggta cactaaaatc
     3001 ttcttcattg tagccttgat tatatacact ttcttttact ttaggtaact taaccttacc
     3061 attatcatat ccttctttaa accaattagc atatttaaca ttacctatag ttgatgtgtc
     3121 aaatctttct cctttgatag cactctcaaa tccttgagta gtgtaatcat tttttaattg
     3181 ttcttgtatt tcgtcataag cattattata agcatctata aattcagaat ctatgttttc
     3241 ttcaaatgtc attttttctt tatctttatt gctatcctct atccctttag ctttatattc
     3301 ttctatcttt ttagtcttat attcattgta tccagttgaa tatccggtat cataagcatt
     3361 ttttaaagct tctttttcat atgtattatt agatttagca cctgtaatcc ctaaattata
     3421 tccagtctct ttagccacct tttcttcctc ttctaattta gccttacctt tttcatatcc
     3481 ttctgagtac tttgattcat atgtatcact ataatctcca ctataatttg atgaactata
     3541 atttccttta tacccatctt catatccttg attatatcct ttttctccaa cagacctctt
     3601 ttgcttttct tcattattaa ccgaagttgt tgttccagaa ctgcttcctc ctccagtata
     3661 aggacaacca ccattattat gtaaatgagc tggataacca ccacaatgat agtgataact
     3721 tcctaatcca gatttatttt tgttatcacg atgacctcct gatgaatcag ttcttcctga
     3781 atgagcatat gaaaagctcc ctacactaat catagttata ctcattgcaa cagctattaa
     3841 ctttttctta aacataatct atttccccct aaaatattct atgattttat tatatcagtc
     3901 aatatattat gtttaaaccg ttaaaattcg acaatgtaaa tatttgatga attgtatttg
     3961 taaataaatc ttttagcatg ttttgaatat atgtagttgt tttgacatca tattcttcaa
     4021 taagtttttt taatatttcg tttttgttaa acataaaaag cgtgcacctg cctttgtata
     4081 gtgatatttg atattttaat tatcactact ttagaagaat acacacttta ttttaaaaat
     4141 cttgatacta tgttattaga aaatggtact aatataaaag atatccagca tatactcgga
     4201 catgctaaaa tttcaacaac tatggataca tattcacatg taactaacaa catgagaaac
     4261 caaacagtaa atgtttttga aagtattgta gagtaaattt gccaccaaaa aacaattacg
     4321 atggcaaatc ggtggcaaac ccacctttat aacatcatat atacagctga aaccctgtta
     4381 ttctctaata agtttaacta cagtctcaac ggtattacca aagactcaca aagctccaga
     4441 cagctatatc aatgtaactg tggactttgg agacagcgtt ggcatgattt ctcttaatag
     4501 tatagtagaa aaggcagacg catacaagcc aaaacagaga ataacttaca agcttataaa
     4561 ggaatatatt cttgagaagt acagctttaa ggtacacaca gcatatattg ccgaagtaaa
     4621 gcgtagcttg ggcttaccta tgtatggata ctcctaatgc agtagaaacg ctaagacaac
     4681 caagaaaaca cccctctcct attcaagtag atgctatcaa agatgcactt gctcattttg
     4741 aagtaatata aaaacacacc ataggaattg ctctttacat taagtaaagg gcaatttttt
     4801 gtacccaaaa atcgaaagga ggaaaaaact tgaacgcaaa aacaatcgcc atcgccaacc
     4861 aaaagggtgg cgtgggcaaa actaccacct gtgtaaattt acaaaggtct accagttgga
     4921 caaggtattc ctgtgtccaa ccttttgctt cacgagcctt ttttatcgcc atacctattg
     4981 gcttgaaatc aagttttctt tcatcttggt acattttaat atcacctcta tatcatttta
     5041 catttcgtgt ggtgatatga gaacgaaaga caattttata ttaggtagtg tataatttca
     5101 tatctgtgaa aaacaaattc gctattattc gtcaatccgt atataataat tatatactct
     5161 tttgcgaaag gaagttaata ttatggatta catttctgtg aaagccgctt ctgaaaaatg
     5221 gggcatatca gaaagacgga tacaaaaatt atgtgaagaa aatcgcatta acggtactga
     5281 gaaatttggt cgagcgtgga tgataccaaa agatgcccaa aagccaattg atggtagata
     5341 taaatcaaca atgaaggtag gtgagattta aaaatgaatt ttattgatat aatgcccaaa
     5401 gaagtaaaac ataaactaca agaaaaaagc tatgctgctg ggaatactat attatatgct
     5461 gaagaagaaa ataatttcgt ttatttttta ttatctggtg ttgctgaagc ttacattcaa
     5521 agttccaatg ggaatttttc aacactttac ttatataaat cgggaagttt ttttggagaa
     5581 atagaacaat tttatgatgg aaaaaagcct gtagaaatta cagcactttc agattgtatt
     5641 gtaaaacgac tttatcgaac ttattttctg gaatggatta aaaatgattt taatgcaaca
     5701 acattcttaa tacaaggatt agccgaaaaa ctaattatta attcccaatt ggtagaaaac
     5761 attttggagc tcacagtaaa agaacgcttg cttcggagta tatcattgca ttatcatcga
     5821 aatacgcttg attcacttaa taaatctcga cttgctaaag aagtaaattc gcctattagg
     5881 agcgttaatc gggcaataga cgaatgtaaa aagcaaggat tactcgattt ttcagataac
     5941 aagtttatta ttataaatcc taaggaactt ctaaaatttc ttccatatat agaatgatga
     6001 aaacagacat cttatttttc aagatgtctg tttttttggg gtcaattgac ctttattatt
     6061 aagctgtcta aatgtaatat acttataagt acaaattctt aggaggtatg tcatgaatgt
     6121 tgctataacc agaattttaa acatcttagg tgatgtaaaa atagtagata agatagtaaa
     6181 tctaaatagc tcggatataa actcttttct attaagtgtc tttcagtcag ctgtaataaa
     6241 aaagacacct caaaacatat tagctgcgtt ttctaccaat cgtttcacta gtccaagcca
     6301 ttttgaccca atcgcctttc ataatttaga ggtgaagttg ttatcccttt cagaaaagct
     6361 tggaattaaa aatctgctac tttctccctc cgctccactt ggtagttgta gtgtttttgg
     6421 atgtgtaaat caaaacaatg taattagtgc attacgggga accgaaactt tatcggaccc
     6481 ttctaatatg atagcaatta ttattgctga cggtttaaaa aagaaaatat tacgtaacga
     6541 agatgctatc catcatgcta caactacaag agtagtacgt gctcaagcct ttgaaggtaa
     6601 agggttttat gctcactttg gaatattttg catagtttcc tctggaaaag ataagggttc
     6661 atattcatgt gaatctgaat tgctaataaa gcatctaaac tattacaaag acctctttac
     6721 aaatattgaa aactcaaaaa taagtatttg cttacggaaa cgtggaggtt atcccgaccg
     6781 agatggattc tttgaaaaaa tgactgctat agtaaagtct tctttaccag agattcaaat
     6841 tactattgat ttaaatagtg aagataatgc ttactacaaa ggaataaatt ttaagattta
     6901 tctacattgc aatgatgatg taattgaaat tgctgatggc ggttttgtaa actggattag
     6961 tgaaatgaca ggaaataaga aaaatcgttg tctgataagt gggataggta ttgatagatt
     7021 attgactttg gaggaataaa atgaaattag aaaattggtc aattgaatat ataaatgact
     7081 tagtaaaaaa tgctaacaat aggaatgtat caaaaaattt gagaaatatt tttccatatc
     7141 catattccgt tgtggatgct gagaaattta tcacacactg ccaaagatgt aataataaac
     7201 atataaatct tgcaatcatt gaagacgaca aagcgattgg aagtgtaggt gtttctattg
     7261 gtgaagatat ttatgaaaaa agtgccgaaa ttggttactg gataggtgaa tcatattggg
     7321 ggcaaggaat tatgacacat gctataaaag aaatgataga actatgcttt tctcaatatg
     7381 atattgttag actttatgct gttgtttttt cacacaataa agcttcttgc aaagtgcttg
     7441 aaaaatgtgg ctttgtattt gaaggaacac ttaaaaattc tatttacaaa gacggcaata
     7501 tttatgacag tcgaatatat gctttggtta aaaaataata attttaacat ctaaatgtac
     7561 cgtcccaacg gaaataaaaa aaaccgttgt tgtggcggca ggtattcata tgccaaaaca
     7621 aaatataagt agcaaagcgg cggttttgaa taacaatcaa agccgcatac gctaccacaa
     7681 agcctactac tctcttgacc gtggtgacgg catcgaacat agtgccttgt ttgtttccct
     7741 ctctcccgat gaaatttacg aacgcaagct cacaaccgag cagctccaca ctgccattgc
     7801 cacattgccg gacaagcagg caaaacgcat ttacgctcat tatttctttg gcatgagtaa
     7861 gaccgctatt gccaaagccg agggtacatc agcaagtagc gtgaaagatg caattcgcag
     7921 agggctatgc agtttagaaa agtatttgaa aaataatttg taagctgccc acgtcgtttg
     7981 cccctctttt tctaaaggta tatgagagga aattttttct ctgatatgcc taacagcata
     8041 attgctcttt gacaatttca tatacgatat tttgagtaca ttacttatgt gaactgaaag
     8101 gcaaagcgac ttaacagaca tcgccatgac agcggtatgg ggaggtgaat ataataccgc
     8161 ctgagcgata aaatggactg ttagaccgag tttagcacac tcgtccgcaa tgacccacat
     8221 aagggataat gatacttcgt gctgttccgc ccccaaaacg atagaaacaa ggggagttcc
     8281 tgcggtatcc gtggcttctg aaaaaacaag ccacggtgct gtgggactat gatagctgtg
     8341 ccaacagcaa ctcaaaatat cccctttgtc ggggtgcgtg gcaaatacgg caaacaggtg
     8401 aatttaatcg caccgcattt ttattgaatg taaatttgcg gtgcatttat tatgctgttt
     8461 ctaataaagt gacaaacttt tcataaataa caaaattgct tatactttat gtaagcaatt
     8521 cacttggagg tgattgcgaa tggaaaatat caaaaacata cagggtgatg cgattaaagc
     8581 ccctgcacct aatcatacat ttgagaaaac aaaagcctat caggatttta taaatgttct
     8641 ttctcaaatt atcgaaaagc atggtgcgga agttttaggc gaaataaacc gtgctaacta
     8701 aaatcatttg cgtgttgatg ttttccaata ctatattaga ggaatgacta tgaataggag
     8761 tggaaaaaaa tgtgtacttt accctcgtgt aagtaccgaa atgcaggttg acggcttcag
     8821 cttggacgga cagaaaaaca gcttaaagcg atttgcagat agagaagaaa tggaaatcgt
     8881 aaatatttat gaagatgcag gcaagtccgg caaatccatt gagggcagaa ctgcctttat
     8941 gcaaatgctc agtgatattg aaaatggttt aggcattgat tatgttttgg tttataaatt
     9001 ctctcgcttt ggacgaaatg ccgccgacat tcttaattct ttggagcata ttcaatcttt
     9061 tggtgtgaat ttgatttgca ttgaagaagg aattgactca tcatagacaa gcggaaagct
     9121 tttaatctct gtgctgtctg ctgttgctga aatagagcgt gaaaacatca ttgaacagac
     9181 tatgaacgga cgaaaagaaa aagctcgtca aggtggttgg aatggtggtt ttgcacccta
     9241 tggctacttt ctcaaagata agcagctttt tatccaagaa gatgaagctg aagcggtacg
     9301 cattatcttt gataaatatg tgaatgggaa tatgggattt tataaaatcg ccaactatct
     9361 taatttacag ggtattcaaa aagtcaaacg ggataacggt acactaaccc aatggagtac
     9421 ccattttgtc agaatgataa ttgacaaccc tgtgtacatg ggaaaaattg cttttggaag
     9481 gcgaacaaga gaaaaggtca aagacactaa gaatgaatac cgccaagtac accaagatga
     9541 ctatatcctt gcagactgtc agcacgatgg tattatcagc gaggagctgt ggacacaggc
     9601 acatgaaaaa cgagaaataa caggtgtgaa agctccctct aaaattggtc gtgacagagc
     9661 acatttgtta agcggtattt taaaatgccc taaatgtggc ggaccgatgt acaccaacaa
     9721 acacgcatgg acaaacaaag acggtacata taaagagatt tactattatg tgtgcagcaa
     9781 ggctcggaca acaagaggga aaagctgtga ctacaaagca atgctcaaaa aaactgatat
     9841 tgagccactt gtcgttgaag caatccgaga tctgattaaa aatgaggatt tttcagctga
     9901 aataaaatcc aaaataggaa aacagattga tacctctacg cttaacagag agaggacgta
     9961 aagctacgcc gaaaagccgt ggaacaggat gccattacct tggagaacat ctacacccta
    10021 ttagcaaatt tcgataaggt gtatgataaa atcagctatg aagagaaaaa atccctaatt
    10081 tcttcgttaa tcaaagaaat agagattttt ccatgtgatg aatcagagct acctctgaag
    10141 tccattttat tcaattttcc ggtgtataag gacggtggag atgtttacga atttttgtgg
    10201 gacaaaagta cgcacgtctc acaatgtgga gtatttggga acatatccat acacttaacc
    10261 atcttaatct cataaccata ccccttaaaa tcaaccaaat caacaaccaa agacttagga
    10321 ttacaagaaa tataaacaat ctccttagca ccaaatccac aaatatccct aatagcatcc
    10381 ttatgaatgc ctggtctagg agggtcaaca ataatcaaat caggcttatc cttcaaatcc
    10441 ttaacaacct tagccacatc cccagcaata aactcacaat tagtaagccc attcaactta
    10501 gcattctcat tagcagcaac aacagcttcc tcaactatct caataccata cactttctta
    10561 gcagcaccag ccataacctg tccaatagac ccagtaccac tatacaagtc aaatacaacc
    10621 ttatcattgt aatctccaat aaaatcccta gcaatactat aaagaacctc agctccctta
    10681 gtattggttt ggaaaaacga aaatggtgaa atcttaaact taagacctaa aatttcttct
    10741 tgtatataat ctcttccata caatactctt aactcatcac attggactgc atctgctaaa
    10801 ccgtcattaa tagtatgaag tacccctacc aactcagctt ttaaatcaat ccctaaaagc
    10861 atatctttaa actcattcat atcaaaatct tcttgagaag aagttactat attaaccata
    10921 atctcattag tatgaatccc ttttctaact acaagatgtc tcaaatatcc tttatgattc
    10981 attgttctat aataaggaag ctttttttca ttgaaaaact ctacactaga aactaatatc
    11041 tttgagaagt cttcatcaac caacatacac tcctcaacag tttgtatatc tatatgtttt
    11101 ccttttttat gaagacctaa tgtcaaaggt ccatttttta cttcatctcc aaaagtatat
    11161 tccatcttat ttctataata tttgttttct ggacttcctt ctatccctaa aaactgaaat
    11221 ccaaataagt cttgctttaa aaataaatcc ataacttgtt tttcttttat ttctaattgt
    11281 ttttcatatg gcacagacaa tagtgtacat cctccacact ctctaaagtg cttgcaagtt
    11341 tcttcagttt caagtggtga gtcctctaat aactctatca tcttaacttc tgctttctta
    11401 cttctaactt ttttaactcc agctctcacc ttttgaccac ttattccacc cttcatatgg
    11461 ataactctat ctccaatttg gcttaaagac gtaccaccaa attccatttt atctacttca
    11521 aattctataa catctttttt cttcacagac attcctccaa tttactatag tatctttatt
    11581 atctttcttc aatcaatttt acttcttcat ctgttaactc tctatactca cctaactcta
    11641 aactttcatc caattttaaa ttgcccatag acaatctttt taagtatact acttttttgc
    11701 ctacgctttc aaacattctt tttacttggt gaaacttacc ttcatgtatt gtaagctcta
    11761 tttctgattc atcatcactt tttaatatat ttaattgtga tggcatagtt ttatatccat
    11821 catcaagcac tacaccttct aaaaaagctt ctacatcttc ttcagtaact actccatcaa
    11881 tttttgcata ataagtctta ggcacatgct tcttaggaga taaaactcta tgagaaagct
    11941 ttccatcatt agttaaaact aataatccct ctgtgtcttt atctaacctt ccaacaggaa
    12001 atggttcaaa agctaagtat gataaatcaa ttaaatctaa cactgttggg tcatatttat
    12061 ctgtagttgc cgatatatat ccatcaggtt tattcatcct gaacctccca tgactaaagt
    12121 cacagggttc ccgcttcata gaattttgca tactaaaata tgtcttacta attctccaca
    12181 ggctatcccc atagttccta cggttcttac ataagttatt tattttgaat tgttattagt
    12241 cttaatcctt cttttagtat attcatacct gcatttatat ctctatcgtg agtttcatta
    12301 caactaggac aaatccattc tcttatattt aaatccttaa cttcctcgtt tttataccca
    12361 catttattac atatttgtga acttgcaaag aatttaccta cttttacaat ttgtctacca
    12421 taccaattag ccttgtattc taattgacga ataaattctg accatgatac atctgaaatt
    12481 aagcgagata gtttatgatt tttaatcata ttctttactt gtaaatcttc tatacaaata
    12541 atatcattct cttttattaa tttagtagat agtttttgta gaaaatcctt tctttgattg
    12601 gctatatgtt cttgaagtct tgcaactttt aatcttgctt tatttctatt taaactaccg
    12661 attgtttttc tcgataattc tctttgtaat ttagcaagtt tgtttaatga cttcttaaga
    12721 tacttaggat tttctataaa gtctccacaa cttgagatac aaaattcttt aattcctaaa
    12781 tctaaaccta tttgattatt agtattttca aatgcttcaa tatctacatc agtacagcat
    12841 aatgatacat aatatctacc acttggctct ttagatatag tagcattaag tattctacct
    12901 ttaggtactt gtttatccct taccttaacc ataccaagtt taggtagttt tatatgttga
    12961 ccacaataca taatgtttcc atttgtaaag ttagttttat atgaaaatct attaattttc
    13021 tttgatttaa atttaggaaa tcctgctttt tctttaaaga attttttata tgcattatct
    13081 aaatctttta aggcgttttg tagtgaaaat ttatcaggtt ctttaagcca atttaattct
    13141 ttttttaaat tggtcaaatc agaactacac tgtttataag taaaagtttc tttattgttt
    13201 ttataaacat ctattctttt agcaagatac ttattatata caaatctaca acatccaaaa
    13261 gttttgttaa ttaactcttg ctgtttttta tttggataca ttctaaactt ataagctttt
    13321 ttcactacta ccacctcact ttacttgctt tttctgatta ttaatatatt tacttatctg
    13381 ctcctctgta ttttctgata cagtagcaac gaaataacta ggattccaaa gatgtctccc
    13441 cataatactg gatgtctata tttgacatac tatatgcata tcctctacca tgagtaac
//
LOCUS       DAEMAE010000096        13450 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEMAE010000096 DAEMAE010000000
VERSION     DAEMAE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.098"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..461
                     /locus_tag="KPH93_002166"
     CDS             <1..461
                     /locus_tag="KPH93_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1688979.1"
                     /translation="SRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKEND
                     IICIEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWYGRQIVNVGKFFASSQIC
                     NKCGYKNEEVKDLNIREWICPSCNETHDRDINAGMNILKEGLRLITIQNK"
     gene            579..1520
                     /locus_tag="KPH93_002167"
                     /pseudo
     CDS             579..1520
                     /locus_tag="KPH93_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(1725..6905)
                     /locus_tag="KPH93_002168"
     CDS             complement(1725..6905)
                     /locus_tag="KPH93_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG1688980.1"
                     /translation="MANKLYSEIINLLEEGRDEFRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIEIGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDMGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLRDI
                     KYQIIEEETKEEESKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKVFIPEGTYSINVSGK
                     DAEKLVGEDSIYNGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYADGGQGGNGEDGK
                     IGATGLTGEMGANAKRDSKRESKAFVENYESSFFMIKAEKGKQGGTGGTGGNGGNAGI
                     GSKSGQIYLIGKAIVLKRNISKKDGSHGMAGKGGSGGKGGSGGLGGIRGIFEVTKTFD
                     KYNINYYFFDRCILDGGKEVPDDGEKLKALYEKHKDWTISKREESGSYGEDGKEGRFG
                     DALKERELLKQEYSLSMDTFFYEFLERFGFSGSKGYRNLRLLCAVGILEADILELSHI
                     YEIFHAVEKVEMQLNREILQKYSGFDKKNKAVISLDDMNIKKEFYKEAKEIYEYLINK
                     LMQSDRIESFIGEKQNFSEWSMILFALEQKVAFYVQMIEENEIGGNRVTDIRSLTEYL
                     DKILIELEQGKKDELKEGYFNTYKESILTQIKYGNQQIEVLKKELENKMLSVNAKMNL
                     LIQEIIKIKENEKEDIEKLKEKQKKLKKQMKKQLILSGIQSALGLLTAFSGVGQACLG
                     AFDSAKSLQENLSAKEEECKWTNTMCDFSDFKLNFDGIDFVVKKQLKEDRKEALGKMT
                     YNLAGIDGVTEVKFDQIKAIMVKSIEDNNYANKANDIQIAKDGLNEGKYEEIYSKIQK
                     KEIGILEANYNNKQQKDKLDALRKSKEAIERQQKISTIKNALSIGSSACFLVNTAISG
                     VNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMAEFKDKTFKESIIPFVEKF
                     DDTVKNQDIFENEISKLKMKEVLTELSTKFASLKMENSEDISIIFKKVQDMMETQIII
                     TDKIDKKKDNLAIGELIYQISDSGNQINEYEKKLIHQIELNQVRYLCQVEAAAFQLWT
                     FPFGSNIVNILNRCHVGITEDENEVVNQAKEQNRQIKEFLDQDVYQWQKTDNHILKVA
                     LTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNQNFDAIKYVTMYANI
                     PNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDITHGLDVKYND
                     SDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSYDSLTNEQKKK
                     LESYFDKPSKYAWSDSIDILKSLGYIDLKGILSKSWYEIKKDIDSDAEIMKHFSILQV
                     DLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYEERLVENIINI
                     "
     gene            complement(6920..9415)
                     /locus_tag="KPH93_002169"
     CDS             complement(6920..9415)
                     /locus_tag="KPH93_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG1688981.1"
                     /translation="MPENNNQLLEEITLLLNKGKAAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMREVEDEDTGISYIECLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGKVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKDSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLETLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSVYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYLKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGRNGADAGYTSYFHGQMFKGYFVKGTSGKKGEKGGAGGNAGRGG
                     NPGNKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYRGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDGITKYHIEFSGGSWNRGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENIIKEKYQGYCSEI"
     gene            complement(9932..10219)
                     /locus_tag="KPH93_002170"
     CDS             complement(9932..10219)
                     /locus_tag="KPH93_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688982.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(10239..10514)
                     /locus_tag="KPH93_002171"
     CDS             complement(10239..10514)
                     /locus_tag="KPH93_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688983.1"
                     /translation="MSLFVGGWSKFRVIEEEDLKVFNEAVGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPQNYLAEIIVFKPLPCDGHNATITSINIIK"
     gene            complement(11133..11312)
                     /locus_tag="KPH93_002172"
     CDS             complement(11133..11312)
                     /locus_tag="KPH93_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688984.1"
                     /translation="MGKDRFEKVYSQGKINVMEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(11625..13064)
                     /locus_tag="KPH93_002173"
     CDS             complement(11625..13064)
                     /locus_tag="KPH93_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1688985.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKVGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNVKRFTSKDFEELNEIIDCMEKNSSNYLIFREQRLKYINKLISKTNNFLIYHF
                     CEIIQHDMIISQLAYSFDNLDNCCIDFFRNKYIEQIRKTYLYGLDGNLIELKKLLTYI
                     YKACRESILKMVENRAPQSRDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEEGAK
                     RLRLLLDAMEFVTITLEDVVFLLGNKIDLIAVDKIETHLRYLQNNCKECKVYPDFVLL
                     CKIVDYTQIYILQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFDILRYLKKG
                     DVESFAESFSNIFKESLQYLKKVISSLNR"
BASE COUNT         4347 a         2387 c         1457 g         5259 t
ORIGIN      
        1 tatcgagaaa aacaatcggt agtttaaata gaaataaagc aagattaaaa gttgcaagac
       61 ttcaagaaca tatagctaat caaagaaagg attttctaca aaaactatct actaaattaa
      121 taaaagagaa tgatattatt tgtatagaag atttacaagt aaagaatatg attaaaaatc
      181 ataaactatc tcgcttaatt tcagatgtat catggtcaga atttattcgt caattagaat
      241 acaaggctaa ttggtatggt agacaaattg taaatgtagg taaattcttt gcaagttcac
      301 aaatatgcaa taaatgtggg tataaaaacg aggaagttaa ggacttaaat ataagagaat
      361 ggatttgtcc tagttgtaat gaaactcacg atagagatat aaatgcaggt atgaatatac
      421 taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa gaaccgtagg
      481 aactatgggg atagcctgtg gagaattagt aagacatatt ttagtatgca aaattctatg
      541 aagcaggaac cctgtgactt tagtcatggg aggttcagaa gacaggcaca ttaacaaaag
      601 ctcaaccaac tgtttcagat ataattacat ttcaaaatta taataaggag gatatgttaa
      661 gacttgcagc ttgcttagaa gaacacttcc cacactccat agcaaatgca gtagtttatg
      721 aagctgaaaa acaagggctt agtcacaaag aaatgcatac agaggtagaa tatattattg
      781 ctcatggtat ttcaacaaag gttgatgata aaaaggttgt aataggtagc aaacattttg
      841 tatttgaaga tgagaagtgt actatcccag acggtgagga ggataagtac aacaatctat
      901 cagatgagta ttcgcaccta tttatggcaa tatcgggtaa attatcagca gttatttgca
      961 taaatgaccc tttacgaaaa gaagcaaaat atgtaatttc aaatttaaga gaatatggaa
     1021 taaaaaagat tgtgatgatg actggagata gcgaaaaaac agctaaatct atagcaagta
     1081 aagttggtgt tgatgaatat tattcagaag tcttaccaga agacaaagcc aattttgtta
     1141 aaagtgaaaa actaaaaggt cgcaaagtaa ttatgattgg tgatggaatc aatgattctc
     1201 ctgctatttc agaatcagat gttggaattg caatgagtga aggtgctgaa atagcaaggg
     1261 agatttcaga tattaccata tcagcagata atttgaataa tttaattgtt ttaaagcaga
     1321 ttagtaataa tcttatgaag agaattgatt taagttctaa gtttattatc ggctttaatt
     1381 taggtctgat tttacttggt gtaggtggat ttgttagacc aagtacatct gcctttttac
     1441 acaacgcatc tactgtcgga ataagtttaa acagtatgac aaatttattg gaaaacacca
     1501 atacttataa ctatcattaa tccctattat ataaaacata tgtatataaa aaatttataa
     1561 ttcatctttt aaaaagaaac tgtcttaaaa gtagcttttt tctaatcaaa attataaaac
     1621 atgctttaca aattttaaac ttaacttatt tctatttttt acaaaaaagg agatgattca
     1681 aattttcatt ttgaaacatc cccttttgta aatttcttta aaacttatat attaattata
     1741 ttttcaacca atctctcttc atagcatttt aaatcatttt gctttagata attaaattca
     1801 gactctacat aaataccaca gcctaccaat tcaattgtca aattaggtat attttccttt
     1861 agtatactag tctgctcata aataattctt tccaataaat caacttgtaa tatagaaaaa
     1921 tgcttcataa tctccgcatc actatctata tctttcttga tttcatacca actctttgac
     1981 aaaattccct tcaaatcaat ataccctaga ctcttcaata tatctatact atcactccat
     2041 gcatacttac taggtttatc aaagtatgat tctagtttct tcttctgctc atttgtaagt
     2101 gaatcataag aaagcttctt attaaaatgt gccttcaaag aaatcttagc catagaatat
     2161 ggacttctac cagagtttaa tcctgcattt ttgaactttt caattccttt atcttctact
     2221 gaagaccata ttgagtttat tgaatctgaa tcattgtatt taacatcaag accatgtgta
     2281 atatctatat agttttgtgg aaatacatat tgataataga catctttttt atcatcatat
     2341 agagagaagt atccactatc atataaatga atatgcacct ttacttttaa actatcctct
     2401 aaaatatttc gtctaaggtt cgggatattt gcatacatgg ttacatattt tatggcatca
     2461 aagttttgat ttaatggaac ttcaaaacta atttcatctc ccgtcaaaag ctttttcact
     2521 tcttcttcat tacaatcata tagagaatat gtattttcaa atcccacacg tgatttccca
     2581 tctaaactac ctgttaatgc taccttcaaa atatggttat ccgttttttg ccattggtaa
     2641 acatcttggt caagaaactc tttaatctgt ctattctgtt ccttggcttg atttacaacc
     2701 tcattttcat cttctgtgat acctacatgg catctattta agatatttac tatattgctt
     2761 ccaaatggaa acgtccatag ttgaaaagct gctgcctcaa cctgacacaa atatctaact
     2821 tgatttaatt cgatttggtg aataagtttt ttttcatatt cattaatttg attaccacta
     2881 tctgatattt gataaattaa ttctccaatt gcaaggttat cttttttctt atcaatctta
     2941 tcagtaatga taatctgagt ttccatcata tcctgtactt ttttgaagat tatagaaatg
     3001 tcctctgaat tttccatttt taaacttgca aactttgtac ttaattcagt taaaacttcc
     3061 ttcatcttta atttacttat ctcattttca aaaatatctt ggtttttaac tgtatcatca
     3121 aatttttcta caaatggtat aatagattct ttaaaagtct tatctttaaa ctctgccatt
     3181 tgagtattta attcatctaa cttcttaata ttattttctt tatctttaat tgcctcttta
     3241 actacctcta ttttgctttc aaaactagac tttacattaa gacaattatt aacaccacta
     3301 attgctgtat taacaagaaa acaagcagaa ctaccaatac ttaatgcatt tttaatagta
     3361 gatatttttt gttgtctttc aatagcctct ttactctttc ttagtgcatc caacttatcc
     3421 ttttgttgtt tattattata attagcttct agtataccta tttctttttt ctggattttt
     3481 gagtaaattt cctcatactt cccttcattt aaaccatctt ttgctatttg aatatcatta
     3541 gctttgtttg cgtagttatt atcctctata gacttaacca tgatagcttt aatttggtca
     3601 aatttaactt ctgttactcc atcaatccct gccaagttat aagtcatttt acctaaagct
     3661 tcctttctat cttcttttaa ctgtttttta actacaaagt ctataccatc aaaatttaac
     3721 ttaaaatctg aaaaatcaca catagtgtta gtccacttgc attcttcttc tttagctgaa
     3781 agattttcct gcaaagattt tgcactatca aatgctccta gacaagcttg tccaacacca
     3841 ctaaatgctg ttagtaagcc caatgcactt tgtattcctg aaagaattaa ttgttttttc
     3901 atttgtttct taagtttttt ctgcttttct tttagcttct ctatgtcctc tttttcattt
     3961 tcttttatct ttataatctc ttgaatcaac agattcattt ttgcattaac agaaagcatt
     4021 ttattctcaa gttctttttt taaaacttca atttgttgat tcccatattt gatttgagtt
     4081 aggatacttt ctttatatgt attgaaatat ccttctttta actcatcctt ctttccttgt
     4141 tccagttcta ttaaaatctt atctaaatac tctgtcaaac tacgaatatc tgtaacacga
     4201 tttcctccaa tttcattttc ttctatcatc tgaacataaa aggctacctt ttgttcaagt
     4261 gcaaataaaa tcatactcca ttcgctaaaa ttctgttttt ctcctataaa agattctatt
     4321 ctatcagact gcatcaattt atttataagg tattcataaa tttcctttgc ctctttataa
     4381 aattctttct ttatattcat atcatccaaa gaaatgactg ctttattttt cttatcaaaa
     4441 ccagaatact tctgaagtat ttctctattt aactgcatct ctactttctc aactgcatga
     4501 aaaatttcat atatatgtga aagttctaaa atatcagctt ctaaaattcc tactgcacac
     4561 aataatctaa ggtttctata ccccttactt ccagaaaagc caaatctctc aaggaactca
     4621 tagaagaatg tatccattga taaagaatat tcttgtttta acagttctct ttcttttagt
     4681 gcatctccaa aacggccttc ttttccatct tcaccatatg aacctgactc ttcacgtttg
     4741 ctaattgtcc agtccttatg tttttcataa agggctttta atttctctcc atcatctggt
     4801 acttctttac ctccgtctaa tatgcatcta tcaaaaaaat agtagtttat attatattta
     4861 tcaaaagttt ttgttacttc aaatatacct ctaattccgc caagacctcc agagcctcct
     4921 tttcctccag aacctccttt tcctgccata ccatgacttc catctttttt gcttatattt
     4981 ctttttaata caatcgcttt tccaatgaga taaatctgcc cagatttgct cccaatacct
     5041 gcatttccac cattacctcc tgtacctcca gttccacctt gttttccttt ttctgctttt
     5101 atcataaaaa agcttgattc ataattttca acaaatgctt tcgactctct tttagaatcc
     5161 cttttagcat ttgcccccat ttctccagtt aatcctgtag ctcctatttt tccatcttct
     5221 ccatttccac cttgtccgcc gtctgcataa agtttgagtt ctcctctttt ttctatctcc
     5281 ttaaaagata ataaaatatc acctgaagtc cctgcattat tgccatttct tccattcata
     5341 ccgttgtaaa tagaatcttc tccaactaat ttttctgcat ccttaccaga tacgttgatt
     5401 gaataagtgc cttctggtat aaatactttt tctgtaagta gtacaatatt cttacctgag
     5461 aagacttctt tctccatatt acaatctatt tttagctcac aatctccata catatatata
     5521 gatgacaaaa tattaagttg atagccagat attattttag tcaaatccga taaattcact
     5581 ttaggtatag gaatatatat agaaagtaca ttttctttct catatgattt tatatatgta
     5641 caactttgaa tcttctctat cctatattcc gcatcttcaa gctttttgct ttcttcttcc
     5701 ttagtttctt cttctattat ctgatattta atatccctta atgtatcttc taaagtgttt
     5761 tttagtaatg attttaacaa ctcacttaaa ccaacaatat gagtctcact acaataacta
     5821 tttaactcta aaagaagacg tgtttcttct aatatattca ctttctgtac gccatcacca
     5881 tatgaaagtt cttgtacaaa ctccataagc tctttcatat tattagttct actacataaa
     5941 tctgtataca tcttgtctaa ttgaatcttt ttatccttta gactatttat agaagataaa
     6001 atggaaatag aatcttccat accttttttg acactttcta tataaggaac taaaagtttt
     6061 tctaggtctt tataggttac atcaacggct ttttgtattt ctattttaca agaagcagta
     6121 agcatcaatc ccatatctgt tttttcaaag gtttcaggta gatttttcat ttcatccaac
     6181 aacttttctg gctgtttatc tagtggatta cataatttaa tagatacacc atctgataat
     6241 gctacattta ataagtacac taattcctta taaccgtcgt aatctttctc ttgactatct
     6301 aggtcttctt ttgctttttt taatatattt cttatttttt cttctgttac attaccgatt
     6361 agtgctttag gtattttatt gataagaaag aagaaatttt ctttatattt tgcataatca
     6421 ccaagcatat ctttcaaatt tttgcctatt tctattaata atgtatgtct agagtccagt
     6481 agggattgat agtctaaaat aactaccatc cctttcacct ttgaagatgt agaaatagtt
     6541 tcccttacaa gcatagcacc agcaatcata tactctgctc cacggttatc aaaaaaacct
     6601 gggcaatcac ataatgaaaa atctctattt gcaatatcta caacttctgg acataaagtc
     6661 ttggaatata caccatttcc aatttcgaca gctgaatctt ttgcagttat atatatttgt
     6721 ccagtctctt catccgtttt ttcttccatt acacagcctt ttaaataatt gatacaagta
     6781 ctctttccaa cgcctgaagc acctaagaat aataaaattg atttttcttt taagtcatat
     6841 tttctaaact cgtctcttcc ttcttccaaa aggtttatga tttcactata aagtttattt
     6901 gccatacatc catccctttc tatatttcag aacaatatcc ttgatatttt tctttaataa
     6961 tattctcttc tacctttgaa acagctgttg ataatttagt gtttggagca ttaaatcctc
     7021 cgttgtctcc tctatctcca tattcagcgt attctcttgc tggtcctgtt ttacctctat
     7081 tccaacttcc gccactaaat tctatatgat atttagttat accatctaac ttataatccc
     7141 aacttgtaaa atatcctgta tagtacacag tcttatctat accacttgga gaaaatgaaa
     7201 gtgtattata tccgcgataa ccaccacttc ctccatatcc acctaaacct ggtctaccat
     7261 ttggacctgc ttctccatct ttcactgtta tatcacaatg attaattatt ctattttttt
     7321 gttcctctga atctgaaatg aagtaaatat tacctttatt accaggattt ccacctcttc
     7381 cagcatttcc accagctcca cctttttcac cttttttacc acttgtacct tttacaaaat
     7441 accctttaaa catctgtccg tggaaataac ttgtataccc tgcatcagct ccattccttc
     7501 cagaagctcc tgtttcacca tttttcccat ctattccttt agaaccattt cctccatttg
     7561 caatcagtgt taaacgataa tttctaattt cttctttaat atataagtaa atattacctg
     7621 aatctctacc atcttcacca tttttaccat acatagaact atctacaagt tctccgctct
     7681 ttccacttac attaattttt acattactac ctacatcaag attgttacaa gcaagaataa
     7741 tatttttctc tgatgcaaag tccattccaa ggttatcatc catataaata gtatctttag
     7801 caaagaacat aaatgtagaa acattgtcat ctacttttct atttttgaga tattccaata
     7861 cactactaat tactaagttt ggaattaatg ctcttatttc aatgcctttc ttatcattaa
     7921 tattaattgt ttttactata aatggattta aaatgtattt accaataaat tctctaatta
     7981 tctcattacc ttcttctcca tatacactta atcttgttat actgtcttct aactcttcat
     8041 tttgtgtaac tgtaacattt gtcaaaattg aattaaagtc acttacacta tatacataat
     8101 cctttttatt tgcaacttcc attaattttt ctctatttgg taatgtaaat acctgttttt
     8161 gcacttcata agaaactaaa actttttcta aattctttct tattataatg aagttttgtt
     8221 ccattatctt cttacttgcc ttttctataa taaattccat atgtacagaa ggtaataaaa
     8281 tttccccagt tccatgttct gtatatttac ttaaagcatc aattatatct aagtttcctt
     8341 ttatcttatt ttgtaatttc atgcaactat cttgtaagaa taagccactt tgtttggcta
     8401 atacgttgat aaatctgtct atagtaagac ttgttgtact ttccaaccag tttttaaata
     8461 cttttacaac ctcatcatat tttgctattt cttgaatcat tgttatactt ccaataatac
     8521 tgataaaatc ttctgtatac gccttaacat aatcttctaa gctagatacg atattgtttt
     8581 gaatttcttt tatcaacgtc tccaaaacta atcttgcatc tgcactaatt ggatactgga
     8641 atgctgatgt cttatcttta acttcatcta attctagtac ttgctttaat aaaccttccc
     8701 tctcatctga tgcaagtggc ttacaaagat gaatattttt tccattatct tttagtaatt
     8761 cttcaaataa ccaagctttt tcctctatat tactatctaa attttcgcat aattgttcat
     8821 attttctttt tataacatta tatatttgtt tttctgttct ccccaagaaa ctttttgaaa
     8881 tgaaaaaagt tatactgtct ttaaaatttt ctttatgaag acacatattt aactgttgga
     8941 acaggtctag tacttttgta agtctagaat ctaatatatc tgcttctggt atcaatacaa
     9001 ttactgcaca tatagatgca gaatttttaa atactttacc taatgaaact gctgcactaa
     9061 tatcatgggc tactccccta ttgtcgctaa aacctgcagt atcacataat tttgcatcca
     9121 ttccttctat tggttttctc atatttacaa tgtttggata taaagtttga gaaacagctc
     9181 ctgaaccaat ctctgccaca gggtctaagc actcaatata tgatatacca gtatcttcat
     9241 cttctacttc cctcattaca catcctgcaa gatagttaat tgcagtactt ttgccacatc
     9301 ctgtcccacc aagaattaga attaaatttt gattttcagc cttatttatt gctgcttttc
     9361 ctttatttaa taagagtgtt atttcttcta aaagttgatt gttattttct ggcatttatt
     9421 ttcccccttg attatatatt ttatttaaat aaagtttaat cccttaagta gattgtaaaa
     9481 tttttagact atatttaaat ttatttttta tttttaataa atttgcattt tcactaaata
     9541 attatttttc tttgtttatt aaaataactg tgttgcagaa tataattttt accaacacag
     9601 ttattttatt taatgcactt attttattgt attttatgta atacagttgt tttttatatt
     9661 atatgtccat tattttccta tggttattac atatatactt ttaattatat ttgtaccatt
     9721 aatttaaaca ttcctatact actgcttaag ttttacacct ataccagctt gtattttatt
     9781 tttatttgct taaataattc aactatactg gtttaaatag gttatcttta tttgcttaaa
     9841 taagttcaac tatactggtt taataggttt cctttagttt aaatatttca cctataccta
     9901 cttgaatagt tcacttttat ttgcttaaat tttataccaa tactggctta aacattatac
     9961 taccatgatt gtattgtcta tcttcaacta caataaaaac aggttctaaa tctttagtag
    10021 atgttatttt taattctaaa aaaacattat cagttggtaa tatttttaca tcataattat
    10081 ttaaattatt attcgtgata ctattaaata catcaccttg acgttcaaaa aaaacattat
    10141 caactattac aaaataaaca tcttcttttc tactattctc aacttctata gtattttgtt
    10201 tagttacatt tagtctcatc ttccacctcc tattaatatt acttgattat attaatactt
    10261 gtaattgttg cattatgtcc atcacaagga agaggtttaa aaacaattat ctctgctaaa
    10321 taattctgtg gaggatttgt aactgaagta gcattgcaaa taaatttaaa atttgttcct
    10381 gctacaactt gagtagcaac aataagtggt tcataatcaa ccccttttaa cattcctaca
    10441 gcttcattaa atacttttaa atcttcttct tcgataaccc taaattttga ccatccacca
    10501 acaaaaagac tcatataaaa acctcctttt atatatattt ttatttatat atatagtagt
    10561 accaattttc tcaatattca atatttcctg tgtaactggt caatgaaaat gcataactgg
    10621 ataaaaatac ctataaaata acttaacttt acatataata agattattct acaatatgaa
    10681 acacatcttg ttttgaatat attctaaata caataatgga tattttcaat tattatactt
    10741 agaaaatatc cattacttaa cttgttttat ttaaaatcgc atagaaactt tattattatt
    10801 ttttactgaa tgaataacca tttcaagtat cataaggtgg tataccctct ccttgaaacc
    10861 acaagaaaca ggacaaactt tacgaatctt ggcttaaaag accacaggta tattccttat
    10921 ttgtgaattg gggtagccat tacaagcaaa ttttacaatt tttatgatga caaaaatcaa
    10981 taaaatcgct tagtatataa cacgaaacat tacttgctac tattaagaat ggttaaatag
    11041 gaagtatgca ttataccaat aaaggaatca ataaagaaag ttaaaacagc cctacaaacc
    11101 aaaaatctat tacatttttc tatatttaaa cgttatttac ttacaattgg agaaataaca
    11161 ggttttcctt ctcggtcaag taaaggcgta aatccggctg cattgccata atagtgaaat
    11221 acataattca cacctgtttc tttatccacc caaatctcca ttacatttat ttttccttgt
    11281 gaatatactt tttcaaatct atcttttccc attttataat cctccatatt tttgattggt
    11341 ctatgccttc ttcacttaag tagaaaaaat acataactta gttttttaat tctaatatga
    11401 gcacgtaaaa atgtcaaaca gcttatttaa tttcataact tcatgttact gtttgtaacc
    11461 ttgcttgttc acttagacta catgacaaca ccatttcacc ctttacagga ttgtattgca
    11521 cacagcagaa atgcttactt tatgggcttt ttcaattaaa aaaaggattt tttctccaca
    11581 gagcatttac cattttttac aatataccta attttaatct catatcagcg atttaatgaa
    11641 gatataacct ttttcaaata ttgtaaagac tccttaaaaa tattggaaaa agattctgca
    11701 aaactctcaa catctccttt ttttaaatac ctaagtatat caaaacatac atcaaaacgt
    11761 gttttcaaaa tttcattata ttcttcatga aaaaatctct ctaaatagat tccaaatatc
    11821 atatattgtt ttaaatgcaa aaatgtttcc tgtaatatat aaatttgtgt ataatctact
    11881 atcttacaaa gtaaaacaaa atctggataa accttacact ctttacaatt attttgtaaa
    11941 taccttaaat gagtttctat tttatctaca gctatcaaat ctattttatt tcctagtaaa
    12001 aatactacat cctcaagagt aattgtaaca aactccatag catccaaaag caaacgcaac
    12061 cttttagctc cttcttctgt atttatataa tcatcatcat attcagaaaa aagtgttata
    12121 taagttccct tgccattaat tgttttagca attcccaatt catttaactc attataggca
    12181 cttctaactg ttggaagaga tacattatat ctattaatta tcgaagtaat tgatggtaga
    12241 caatctccta ttttaatatt tccttcaaaa atctgacaaa ttacatctga aacaattaag
    12301 tcatataaat aacatttttc caatgttaca gaattaaaac tacttgtatc tctactttgt
    12361 ggagcacgat tttctaccat tttaagtatg gattctctac aagctttata gatatatgtt
    12421 aatagttttt ttaattcaat taaatttcca tccaatccat ataagtaagt ttttcttatc
    12481 tgctctatgt atttattacg aaaaaagtct atacaacaat tatccaagtt atcgaaacta
    12541 taggccaact gtgaaataat catatcatgc tgaataatct cacaaaaatg ataaatcagg
    12601 aaattattcg ttttacttat tagtttattt atatatttta gcctctgctc tctaaagatt
    12661 aaataattag aactattttt ttccatacaa tcgataatct catttaattc ttcaaagtct
    12721 ttagatgtaa atctcttaac attgtacata gcaatagaag gaaatattaa actaaaaatc
    12781 ttatatattt ctgatatttg taaaatcttc tcatataata agtcagattc tattgattgt
    12841 ttactcgtac tctggattaa aactggtcta gaaccaactt ttgtctctat aaatcccttt
    12901 tctgagagca tttttattac agtttttata gtattttttg ataccatata ttcttctgac
    12961 atgcttttta tagaatcaag cctatctcca atagtaattt taccagtata aatttgttga
    13021 attatatgct caaaaataat taaatattgt ggttttctct tcatattttt cctcttcgat
    13081 aaattagatt tattgttttt tgtaaggtct atttactttt atttaatagg gcaaaatagt
    13141 tgcttatttt ttcctcataa tctataatta atataaacca tttatatata tggtctaagt
    13201 tttataatac tgtaatgcag taaaatagca tatttcagtt atactacaga aaattaacat
    13261 accattgtag tatattaatt ggaataattt taagtacttc aaaattccag caaaacatat
    13321 atatgttttt gatattatgt atattcgtta atagaatagc tatacttgtt ttacataata
    13381 aattatatga tacttgctat atgtaattac tatatttaat acacataact attatacttg
    13441 ctatatgtaa
//
LOCUS       DAEMAE010000097        13437 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEMAE010000097 DAEMAE010000000
VERSION     DAEMAE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.099"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2955
                     /gene="tcdB"
                     /locus_tag="KPH93_002174"
                     /gene_synonym="toxB"
     CDS             <1..2955
                     /gene="tcdB"
                     /locus_tag="KPH93_002174"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG1688986.1"
                     /translation="ILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYK
                     LLISGELKILMLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLF
                     VSELPDVVLISKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSL
                     TLQDEKTIKLNSVHLDESGVAEILKFMNRKGSTNTSDSLMSFLESMNIKSIFVNFLQS
                     NIKFILDANFIISGTTSIGQFEFICDENNNIQPYFIKFNTLETNYTLYVGNRQNMIVE
                     PNYDLDDSGDISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPE
                     VIVLDANYINEKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDK
                     TLANKLSFNFSDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMV
                     SGLIYINDSLYYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQ
                     SGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFEDNYRGAVEW
                     KELDGEMHYFSPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMK
                     VGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNN
                     KIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFV
                     TINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDN
                     IYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKACKGINLI
                     DDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVF
                     NTPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYY
                     FDPDTAQLVISE"
     gene            3077..3577
                     /gene="tcdE"
                     /locus_tag="KPH93_002175"
     CDS             3077..3577
                     /gene="tcdE"
                     /locus_tag="KPH93_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG1688987.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            3906..4079
                     /locus_tag="KPH93_002176"
     CDS             3906..4079
                     /locus_tag="KPH93_002176"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688988.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICS
                     IIQSPYLIEESIN"
     gene            4296..4436
                     /locus_tag="KPH93_002177"
     CDS             4296..4436
                     /locus_tag="KPH93_002177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688989.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YL"
     gene            4470..4727
                     /locus_tag="KPH93_002178"
     CDS             4470..4727
                     /locus_tag="KPH93_002178"
                     /inference="COORDINATES: protein motif:HMM:NF024320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688990.1"
                     /translation="MNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKRL"
     gene            4697..5122
                     /locus_tag="KPH93_002179"
     CDS             4697..5122
                     /locus_tag="KPH93_002179"
                     /inference="COORDINATES: protein motif:HMM:NF024321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688991.1"
                     /translation="MLSQYTKKAIVESSTTEALQLLEEEIQNPQFDNMKFYKKRMEFI
                     YDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDETLLESYRTNSLRKINSNHG
                     IDIRANSLFTEQELLNIYSQDLLNRGNLAAASDIVRLLA"
     gene            5155..6162
                     /locus_tag="KPH93_002180"
     CDS             5155..6162
                     /locus_tag="KPH93_002180"
                     /inference="COORDINATES: protein motif:HMM:NF024321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688992.1"
                     /translation="MLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTS
                     ENFDKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDIEIKIAFALGSVINQALISK
                     QGSYLTNLVIEQIKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLT
                     KIAPYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPEN
                     NLSQLTEQEINSLWSFDQASAKYQFEKCVRDYTGGSLSEDNGVDFNKNTALDKNYLLN
                     NKMPSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSY
                     F"
     gene            6180..8684
                     /locus_tag="KPH93_002181"
     CDS             6180..8684
                     /locus_tag="KPH93_002181"
                     /inference="COORDINATES: protein motif:HMM:NF023141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688993.1"
                     /translation="MLELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSL
                     SNEISSFLDTIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPY
                     VNKNSITIGANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKL
                     KAKSKNIPGLASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVK
                     NIIHNSIDDLIDEFNLLENVSNELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINK
                     SNGESIYVETEKEIFSKYSEHITKEISTIKNSIITDVNSNLLDNIQLDHTSQVNTLNA
                     AFFIQSLIDYSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTI
                     NVLPTITEGIPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAA
                     TVASIVGIGAEVTIFLLPIAGISAGILSLVNNELILHDKATSVVNYFNHLSESKKYGP
                     LKTEDDKILVPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSI
                     SSHIPSLSIYSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLGNDGTRLL
                     DSIRDLYPGKFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRN
                     KLSYSFDGAGGTYSLLLSSYPISTNINLSKYDLWIFNIDNEVREISIENGTIKKGKLI
                     KDVLSKIDINKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYS
                     LLLSGDKNYLISNLSNTIEKINTLGLDSKKYSVQLH"
     gene            8668..10605
                     /locus_tag="KPH93_002182"
     CDS             8668..10605
                     /locus_tag="KPH93_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBG1688994.1"
                     /translation="MYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILEFYNGSTLE
                     FNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVNKNQVKVNGLYLNES
                     VYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTLVGKTNLGY
                     VEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDISTSLDFSYE
                     PLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSSS
                     FEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTKSFNKMSIDFKDIKKLSLGY
                     IMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEASKLVKGLININNSLFYFDPIESNL
                     VTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPDGFEYFAPA
                     NTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATG
                     WQTIDGKKYYFNTNTSIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTD
                     ANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAVTGWQTINGNVYYFMPDTAMAAAGGLF
                     EIDGVIYFFGVDGVKAPGIYG"
     gene            complement(10940..11638)
                     /gene="tcdC"
                     /locus_tag="KPH93_002183"
     CDS             complement(10940..11638)
                     /gene="tcdC"
                     /locus_tag="KPH93_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG1688995.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLVFILGVFFG
                     NISSPACSEDHEKVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            12055..12300
                     /locus_tag="KPH93_002184"
     CDS             12055..12300
                     /locus_tag="KPH93_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG1688996.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(12518..12787)
                     /locus_tag="KPH93_002185"
     CDS             complement(12518..12787)
                     /locus_tag="KPH93_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1688997.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(12919..13221)
                     /locus_tag="KPH93_002186"
     CDS             complement(12919..13221)
                     /locus_tag="KPH93_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1688998.1"
                     /translation="MEYSKGFKVIYSLVEYSKRFKVIYCVMEYSKRFKVIYCVMEYLK
                     GFKVIYGVMEYSKRFKAIYDVMEYSKRFKSIYSFTKYSNGFEVIYCALKYSKIFLE"
BASE COUNT         5329 a         1540 c         1937 g         4631 t
ORIGIN      
        1 atcttaaata gccatgagat taatttttct ggtgaggtaa atggaagtaa tggatttgtt
       61 tctttaacat tttcaatttt agaaggaata aatgcaatta tagaagttga tttattatct
      121 aaatcatata aattacttat ttctggcgaa ttaaaaatat tgatgttaaa ttcaaatcat
      181 attcaacaga aaatagatta tataggattc aatagcgaat tacagaaaaa tataccatat
      241 agctttgtag atagtgaagg aaaagagaat ggttttatta atggttcaac aaaagaaggt
      301 ttatttgtat ctgaattacc tgatgtagtt cttataagta aggtttatat ggatgatagt
      361 aagccttcat ttggatatta tagtaataat ttgaaagatg tcaaagttat aactaaagat
      421 aatgttaata tattaacagg ttattatctt aaggatgata taaaaatctc tctttctttg
      481 actctacaag atgaaaaaac tataaagtta aatagtgtgc atttagatga aagtggagta
      541 gctgagattt tgaagttcat gaatagaaaa ggtagtacaa atacttcaga ttctttaatg
      601 agctttttag aaagtatgaa tataaaaagt attttcgtta atttcttaca atctaatatt
      661 aagtttatat tagatgctaa ttttataata agtggtacta cttctattgg ccaatttgag
      721 tttatttgtg atgaaaataa taatatacaa ccatatttca ttaagtttaa tacactagaa
      781 actaattata ctttatatgt aggaaataga caaaatatga tagtggaacc aaattatgat
      841 ttagatgatt ctggagatat atcttcaact gttatcaatt tctctcaaaa gtatctttat
      901 ggaatagaca gttgtgttaa taaagttgta atttcaccaa atatttatac agatgaaata
      961 aatataacgc ctgtatatga aacaaataat acttatccag aagttattgt attagatgca
     1021 aattatataa acgaaaaaat aaatgttaat atcaatgatc tatctatacg atatgtatgg
     1081 agtaatgatg gtaatgattt tattcttatg tcaactagtg aagaaaataa ggtgtcacaa
     1141 gttaaaataa gattcgttaa tgtttttaaa gataagactt tggcaaataa gctatctttt
     1201 aactttagtg ataaacaaga tgtacctgta agtgaaataa tcttatcatt tacaccttca
     1261 tattatgagg atggattgat tggctatgat ttgggtctag tttctttata taatgagaaa
     1321 ttttatatta ataactttgg aatgatggta tctggattaa tatatattaa tgattcatta
     1381 tattatttta aaccaccagt aaataatttg ataactggat ttgtgactgt aggcgatgat
     1441 aaatactact ttaatccaat taatggtgga gctgcttcaa ttggagagac aataattgat
     1501 gacaaaaatt attatttcaa ccaaagtgga gtgttacaaa caggtgtatt tagtacagaa
     1561 gatggattta aatattttgc cccagctaat acacttgatg aaaacctaga aggagaagca
     1621 attgatttta ctggaaaatt aattattgac gaaaatattt attattttga agataattat
     1681 agaggagctg tagaatggaa agaattagat ggtgaaatgc actattttag cccagaaaca
     1741 ggtaaagctt ttaaaggtct aaatcaaata ggtgatgata aatactattt caattctgat
     1801 ggagttatgc aaaaaggatt tgttagtata aatgataata aacactattt tgatgattct
     1861 ggtgttatga aagtaggtta cactgaaata gatggcaagc atttctactt tgctgaaaac
     1921 ggagaaatgc aaataggagt atttaataca gaagatggat ttaaatattt tgctcatcat
     1981 aatgaagatt taggaaatga agaaggtgaa gaaatctcat attctggtat attaaatttc
     2041 aataataaaa tttactattt tgatgattca tttacagctg tagttggatg gaaagattta
     2101 gaggatggtt caaagtatta ttttgatgaa gatacagcag aagcatatat aggtttgtca
     2161 ttaataaatg atggtcaata ttattttaat gatgatggaa ttatgcaagt tggatttgtc
     2221 actataaatg ataaagtctt ctacttctct gactctggaa ttatagaatc tggagtacaa
     2281 aacatagatg acaattattt ctatatagat gataatggta tagttcaaat tggtgtattt
     2341 gatacttcag atggatataa atattttgca cctgctaata ctgtaaatga taatatttac
     2401 ggacaagcag ttgaatatag tggtttagtt agagttggtg aagatgtata ttattttgga
     2461 gaaacatata caattgagac tggatggata tatgatatgg aaaatgaaag tgataaatat
     2521 tatttcgatc cagaaactaa aaaagcatgc aaaggtatta atttaattga tgatataaaa
     2581 tattattttg atgagaaggg cataatgaga acgggtctta tatcatttga aaataataat
     2641 tattacttta atgagaatgg tgaaatgcaa tttggttata taaatataga agataagatg
     2701 ttctattttg gtgaagatgg tgtcatgcag attggagtat ttaatacacc agatggattt
     2761 aaatactttg cacatcaaaa tactttggat gagaattttg agggagaatc aataaactat
     2821 actggttggt tagatttaga tgaaaagaga tattatttta cagatgaata tattgcagca
     2881 actggttcag ttattattga tggtgaggag tattattttg atcctgatac agctcaatta
     2941 gtgattagtg aatagataaa aatatgttaa atatatcctc ttatacttaa atatataaaa
     3001 ataaacaaaa tgatacacta cataaagtgt tctatctaat atgaagattt accaataaaa
     3061 aggtggacta tgatgaatgc acagtagttc acctttttat atttctaatg gtaacaaaat
     3121 atttttttat ataaacctag gaggcgttat gaatatgaca atatcttttt tatcagagca
     3181 tatatttata aagttagtaa ttttaactat atcatttgat acattattag gatgtttaag
     3241 tgcaataaaa agtcgtaaat ttaattctag ttttggaata gatggaggaa tcagaaaagt
     3301 agcaatgata gcatgtatat tttttttatc agtagttgac attcttacaa agtttaactt
     3361 tttatttatg ttaccacaag attgtatcaa ttttttaaga ctaaaacatc ttggaatatc
     3421 tgaatttttc tctattttat ttattttata tgaaagtgta agtatattaa aaaatatgtg
     3481 cttatgtgga ttaccagtac ctaagagatt aaaggaaaaa atagcagttt tactagatgc
     3541 aatgacagat gaaatgaatg ctaaggatga aaagtaagta atggtagata taataaagat
     3601 attaacaaat aaaaagtgtt atccaaataa gaatagctga aagttatcat aattcatgaa
     3661 actaataatg aaaacgaggg agcagatgcc aagagacaca caagtattaa atacatataa
     3721 tttcgaagca agtgttcatt actatatgga tgacaaggta gtatatcaaa cattggttca
     3781 caaagatggt gcatggtcag ttggtaaaat ctattaagct acattagtta cagatatcac
     3841 aaactataat agttaaacat agaaatatgt gtaaattgtg atggaaatta ttcaaaaaca
     3901 caaaaatacg tgatgaagga caaaatgata gagaaaataa gtatcaaacc ttaataaatg
     3961 atttaattga tagtttaaaa gttataggaa aaatatataa agaaataaaa acattaaaaa
     4021 aatataagat atgttctatc atacaatctc cttatctaat agaagagtca attaactaat
     4081 tgagtatctt taaattgaaa tgttaggaag tgatttaaat atgaaaactt aaattataaa
     4141 aaatcaatat taatttattt taaaaaaata gaaaggagtg tataagattt attttcaaag
     4201 tttaaaaaca agaaaatcaa tttaaatttc agaaggaata aatgtggtta tagaagtgga
     4261 tttattatca aaaataataa tactaggagg tttttatgtc tttaatatct aaagaagagt
     4321 taataaaact cgcatatagc attagaccaa gagaaaatga gtataaaact atactaacta
     4381 atttagacga atataataag ttaactacaa acaataatga aaataaatat ttataattaa
     4441 aaaaactaaa tgaatcaatt gatgttttta tgaataaata taaaaattca agcagaaata
     4501 gagcactctc taatctaaaa aaagatatat taaaagaagt aattcttatt aaaaattcca
     4561 atacaagtcc tgtagaaaaa aatttacatt ttgtatggat aggtggagaa gtcagtgata
     4621 ttgctcttga atacataaaa caatgggctg atattaatgc agaatataat attaaactgt
     4681 ggtatgatag cgaagcattc ttagtcaata cactaaaaag gctatagttg aatcttctac
     4741 cactgaagca ttacagctac tagaggaaga gattcaaaat cctcaatttg ataatatgaa
     4801 attttacaag aaaaggatgg aatttatata tgatagacaa aaaaggttta taaattatta
     4861 taaatctcaa atcaataaac ctacagtacc tacaatagat gatattataa agtctcatct
     4921 agtatctgaa tataatagag atgaaacttt attagaatca tatagaacaa attctttgag
     4981 aaaaataaat agtaatcatg ggatagatat cagagctaat agtttgttta cagaacaaga
     5041 gttattaaat atttatagtc aggatttgtt aaatcgtgga aatttagctg cagcatctga
     5101 catagtaaga ttattagcct aaaaaatttt ggcggagtat atttagatgt tgatatgctt
     5161 ccaggtattc actctgattt atttaaaaca atatctagac ctagctctat tggactagac
     5221 cgttgggaaa tgataaaatt agaggctatt atgaagtata aaaaatatat aaataattat
     5281 acatcagaaa actttgataa acttgatcaa caattaaaag ataattttaa actcattata
     5341 gaaagtaaaa gtgaaaaatc tgagatattt tctaaattag aaaatttaaa tgtatctgat
     5401 attgaaatta aaatagcttt cgctttaggc agtgttataa atcaagcctt gatatcaaaa
     5461 caaggttcat atcttactaa cctagtaata gaacaaataa aaaatagata tcaattttta
     5521 aaccaacacc ttaacccagc catagagtct gataataact tcacagatac tactaaaatt
     5581 tttcatgatt cattatttaa ttcagctact gcagaaaact ctatgttttt aacaaaaata
     5641 gcaccatact tacaagtagg ttttatgcca gaagctcgct ccacaataag tttaagtggt
     5701 ccaggagctt atgcatcagc ttactatgat ttcataaatt tacaagaaaa tactatagaa
     5761 aaaactttaa aagcatcaga tttaatagaa tttaaattcc cagaaaataa tctatctcaa
     5821 ttgacagaac aagaaataaa tagtctatgg agctttgatc aagcaagtgc aaaatatcaa
     5881 tttgagaaat gtgtaagaga ttatactggt ggatctcttt ctgaagacaa tggggtagac
     5941 tttaataaaa atactgccct cgacaaaaac tatttattaa ataataaaat gccatcaaac
     6001 aatgtagaag aagctggaag taaaaattat gttcattata tcatacagtt acaaggagat
     6061 gatataagtt atgaagcaac atgcaattta ttttctaaaa atcctaaaaa tagtattatt
     6121 atacaacgaa atatgaatga aagtgcaaaa agttactttt aagtgatgat ggagaatcta
     6181 ttttagaatt aaataaatat aggatacctg aaagattaaa aaataaggaa aaagtaaaag
     6241 taacctttat tggacatggt aaagatgaat tcaacacaag cgaatttgct agattaagtg
     6301 tagattcact ttccaatgag ataagttcat ttttagatac cataaaatta gatatatcac
     6361 ctaaaaatgt agaagtaaac ttacttggat gtaatatgtt tagttatgat tttaatgttg
     6421 aagaaactta tcctggtaag ttgctattaa gtattatgga caaaattact tccactttac
     6481 cttatgtaaa taaaaattct attactatag gagcaaatca atatgaagta agaattaata
     6541 gtgagggaag aaaagaactt ctagctcact caggtaaatg gataaataaa gaagaagcta
     6601 ttatgagcga tttatctagt aaagaataca ttttttttga ttctatagat aataagctaa
     6661 aagcaaagtc caagaatatt ccaggattag catcaatatc agaagatata aaaacattat
     6721 tacttgatgc aagtgttagt cctgatacaa aatttatttt aaataatctt aagcttaata
     6781 ttgaatcttc tattggtgat tacatttatt atgaaaaatt agagcctgtt aaaaatataa
     6841 ttcacaattc tatagatgat ttaatagatg agttcaatct acttgaaaat gtatctaatg
     6901 aattatatga attaaaaaaa ttaaataatc tagatgagaa gtatttaata tcttttgaag
     6961 atatctcaaa aaataattca acttactctg taagatttat taacaaaagt aatggtgagt
     7021 caatttatgt agaaacagaa aaagaaattt tttcaaaata tagcgaacat attacaaaag
     7081 aaataagtac tataaagaat agtataatta cagatgttaa tagtaattta ttggataata
     7141 tacagttaga tcatacttct caagttaata cattaaacgc agcattcttt attcaatcat
     7201 taatagatta tagtagcaat aaagatgtac tgaatgattt aagtacctca gttaaggttc
     7261 aactttatgc tcaactattt agtacaggtt taaatactat atatgactct atccaattag
     7321 taaatttaat atcaaatgca gtaaatgata ctataaatgt actacctaca ataacagagg
     7381 ggatacctat tgtatctact atattagacg gaataaactt aggtgcagca attaaggaat
     7441 tactagacga acatgaccca ttactaaaaa aagaactaga agctaaggtg ggtgttttag
     7501 caataaatat gtcattatct atagctgcaa cggtagcttc aattgttgga ataggtgctg
     7561 aagttactat tttcttatta cctatagctg gtatatctgc aggaatactt tcattagtta
     7621 ataatgaatt aatattgcat gataaggcaa cttcagtggt aaactatttt aatcatttgt
     7681 ctgaatctaa aaaatatggc cctcttaaaa cagaagatga taaaatttta gttcctattg
     7741 atgatttagt aatatcagaa atagatttta ataataattc gataaaacta ggaacatgta
     7801 atatattagc aatggagggg ggatcaggac acacagtgac tggtaatata gatcactttt
     7861 tctcatctcc atctataagt tctcatattc cttcattatc aatttattct gcaataggta
     7921 tagaaacaga aaatctagat ttttcaaaaa aaataatgat gttacctaat gctccttcaa
     7981 gagtgttttg gtgggaaact ggagcagttc caggtttaag atcattggga aatgatggaa
     8041 ctagattact tgattcaata agagatttat acccaggcaa attttactgg agattctatg
     8101 cttttttcga ttatgcaata actacattaa aaccagttta tgaagacact aatattaaaa
     8161 ttaaactaga taaagatact agaaacttca taatgccaac tataactact aacgaaatta
     8221 gaaacaaatt atcttattca tttgatggag caggaggaac ttactcttta ttattatctt
     8281 catatccaat atcaacgaat ataaatttat ctaaatatga tttatggata tttaatattg
     8341 ataatgaagt aagagaaata tctatagaaa atggtactat taaaaaagga aagttaataa
     8401 aagatgtttt aagtaaaatt gatataaata aaaataaact tattataggc aatcaaacaa
     8461 tagatttttc aggcgatata gataataaag acagatatat attcttgact tgtgagttag
     8521 atgataaaat tagtttaata atagaaataa atcttgttgc aaagtcttat agtttgttat
     8581 tgtctggaga taaaaattat ttgatatcca atttatctaa tactattgag aaaataaata
     8641 ctttaggcct agatagtaaa aaatatagtg tacaattaca ctgatgaatc taataataaa
     8701 tattttggag ctatatctaa aacaagtcaa aaaagcataa tacattataa aaaagacagt
     8761 aaaaatatat tagaatttta taatggcagt acattagaat ttaacagtaa agattttatt
     8821 gctgaagata taaatgtatt tatgaaagat gatattaata ctataacagg aaaatactat
     8881 gttgataata atactgataa aagtatagat ttctctattt ctttagttaa taaaaatcaa
     8941 gtaaaagtga atggattata tttaaatgaa tccgtatact catcttacct tgattttgtg
     9001 aaaaattcag atggacacca taatacttct aattttatga atttattttt ggataatata
     9061 agtttctgga aattgtttgg gtttgaaaat ataaattttg taatcgataa atactttacc
     9121 cttgttggta aaactaatct tggatatgta gaatttattt gtgacaataa taaaaatata
     9181 gatatatatt ttggtgaatg gaaaacatcg tcatctaaaa gcactatatt tagcggaaat
     9241 ggtagaaatg ttgtagtaga gcctatatac aatcctgata cgggtgaaga tatatctact
     9301 tcactagatt tttcttatga acctctctat ggaatagata gatatatcaa taaagtattg
     9361 atagcacctg atttatatac aagtttaata aatattaata ccaattatta ttcaaatgag
     9421 tactatcctg agattatagt tcttaaccca aatacattcc acaaaaaagt aaatataaat
     9481 ttagatagtt cttcttttga gtataaatgg tctacagaag gaagtgactt tattttagtt
     9541 agatacttag aagaaagtaa taaaaaaata ttacaaaaaa taagaatcaa aggtatctta
     9601 tctaatacta aatcatttaa taaaatgagt atagatttta aagatattaa aaagctatca
     9661 ttaggatata taatgagtaa ttttaaatca tttaattctg aaaatgaatt agatagagat
     9721 catttaggat ttaaaataat agataataaa acttattact atgatgaagc tagtaaatta
     9781 gttaaaggat taatcaatat aaataattca ttattctatt ttgatcctat agaatctaac
     9841 ttagtaactg gatggcaaac tatcaatggt aaaaaatatt attttgatat aaatactgga
     9901 gcagcttcaa ctagttataa aattattaat ggtaaacact tttattttaa taataatggt
     9961 gtgatgcagt taggagtatt taaaggacct gatggatttg agtattttgc acctgccaat
    10021 actcagaata ataacataga aggtcaggct atagtttatc aaagtaaatt cttaactttg
    10081 aatggcaaaa aatattattt tgataatgac tcaaaagcag ttactggatg gcaaaccatt
    10141 gatggtaaaa aatattactt taatcttaac actgctgaag cagctactgg atggcaaact
    10201 attgatggta aaaaatatta ctttaatact aacacttcca tagcttcaac tggttataca
    10261 attattaatg gtaaacattt ttattttaat actgatggta ttatgcagat aggagtgttt
    10321 aaaggaccta atggatttga atactttgca cctgctaata cggatgctaa caatatagaa
    10381 ggtcaagcta tacgttatca aaatagattc ctatatttac atgacaatat atattacttt
    10441 ggtaataatt caaaagcagt tactggatgg caaactatta atggtaatgt atattacttt
    10501 atgcctgata ctgctatggc tgcagctggt ggacttttcg agattgatgg tgttatatat
    10561 ttctttggtg ttgatggagt aaaagcccct gggatatatg gctaaaatat atgtgtgaca
    10621 aaaaattatt cctgtgctac taagaaatta tttttatata ataaatattg agatttaatt
    10681 aaaagtcatg tgttattgta atacatgact tttagttaaa atttttctat catttaataa
    10741 tctattattc ttgactattt tataataaaa ttcgtatatg gaaatattaa tactaaataa
    10801 ttaatagttg atagaaaata gataatgtgc taaaagcaaa aactaattta gagccttgta
    10861 actgtttatt tgcaattata aaaacatctt taaacatatt gactataata taaaatatta
    10921 actataatac aaaacaatat taattaattt tctctacagc tatccctggt atagttatat
    10981 ttccacccat agttgattca taagttatag ttccagcact tatacctctt atagttatgt
    11041 aatcatcctc taatactctt gaaggagtta ttgatttttt ataactacat agtacaacct
    11101 tatcataatt tccagacaca gctaatctta tttgcacctc atcaccatct tcaataactt
    11161 gaataacctt accttcaaat tttacctttt tgtacttata atcatcaggt gttctagcta
    11221 attggtcata agtaatacca gtatcatatc ctttcttctc ttcttcttct tttttacgtt
    11281 gttcttcagc ctttttagct tcttcagctt ttttagcttc ttcagcttta cgttgatttt
    11341 cagcttcaat agctttcttt tcgtcgtctt tcattttgaa ccatggttca gcatcagaca
    11401 atttgctatt taaagtttct atttctgttt tttgagaatc tataactgat gtttgattag
    11461 aaatgacctt ctcatggtct tcagaacaag ctggtgagga tatattgcca aaaaacacgc
    11521 ctaaaataaa taccagtaga gcaataccct ttgtgctctt aaagaattta attattttct
    11581 tagagcttcc ttttccttca ttactaaatt cgttaccctc atttttttta gaaaacataa
    11641 tacaatctcc cttttattag atatatttta ttttaaaagt taaaaactta aaataactag
    11701 ttgactaaaa attatatatt catattttcc taaaatatat ttcccctaag cataatttta
    11761 caaaataaag taagaaaatt caatgtattt tgaaaaataa ggtaaaaatg tcagattata
    11821 atataatttt cttgaaggtg aattttatat attaaatgaa acataaagta ttttatttgt
    11881 ataaaactat attatgatgt aattaagtga ctcagtactt tagaggtggt aaaatataca
    11941 tagatgaaag agcataaata aattgatata ataaaaatta aatattaact ataataaaaa
    12001 aatatctggt actaagtata atacaaaaat aatatgccag aaagaaggcg aagtatggga
    12061 gaactacaaa aaattccagg agtagggaaa gcaacagaaa aatctttaat catgttagga
    12121 tatacaacta ttaaatcact taaagatgca aacccagcac aaatgtatga aaaggaatgt
    12181 ttaatgcgtg gacaacatat agacagatgt cagttatatg tatacagatg tgcagtatat
    12241 tttgcatcaa ctgaaaatcc agaaccagaa aaattaaagt ggtggtattg gaaagattaa
    12301 aaaatcaaaa aagttagata taaaaaagag ttgaatatcc gttttaaata ataaaaacta
    12361 atactcaact cttttttagt cgtaaatttt atgtaaatct atttgtatgg ctaaatatac
    12421 ccatcaataa atgtataaat ataccaatca acaaatgact aaacatacta tctaacaaag
    12481 tattaaataa attgcttaac aaaacattct aaaacaatta attagaaact gtttcttcaa
    12541 gccattttct gcattgctca atatttacca tagggtcaaa accttggtct tttcccttat
    12601 ttaaaagcat atcacaaggg aaagtatcac attcaccaca atgattaagt tccttacttt
    12661 cacagcaagt tttaacctta cattctccac cccaaaatgg tttttccata gttgggcaac
    12721 cagtgcaatt tacttgttct ttttttgtac aactgtcaca acagacacca catcttgatt
    12781 caaacatatt caaacatcct ttccattatt taaaaatatt ataaaataat tttagatgac
    12841 tacaaaagtg caaataattg attttaagtc atctaaacat taaacataaa attattagta
    12901 ctggtaaatt tataaaaact attccaaaaa tatttttgaa tatttcaaag cacaataaat
    12961 tacttcaaat ccatttgaat atttagtaaa actatagatt gatttaaatc tctttgaata
    13021 ttccataaca tcatagattg ctttaaatct ctttgaatat tccataacac catagattac
    13081 tttaaatccc tttaaatatt ccataacaca ataaattact ttaaatctct ttgaatattc
    13141 cataacacaa taaattactt taaatctctt tgaatattcc acaagactat agattacttt
    13201 aaatcccttt gaatattcca taacactata aattactttt agcttttata taaccataaa
    13261 gtttttgtct ggaactttaa atggagtttt atatcaaatt tgcattccat caaataacca
    13321 aacttaattt aattacacat ttcaagggtc aaatatttat tcaatatatt tgataattct
    13381 atgttatagc cataattaaa tataggttat taaagttaaa tatttattta atatatt
//
LOCUS       DAEMAE010000098        13400 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEMAE010000098 DAEMAE010000000
VERSION     DAEMAE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.100"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            320..778
                     /locus_tag="KPH93_002187"
     CDS             320..778
                     /locus_tag="KPH93_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1688999.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFIAVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            839..1114
                     /locus_tag="KPH93_002188"
     CDS             839..1114
                     /locus_tag="KPH93_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1689000.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1173..2447
                     /locus_tag="KPH93_002189"
     CDS             1173..2447
                     /locus_tag="KPH93_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG1689001.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2577..3629
                     /locus_tag="KPH93_002190"
     CDS             2577..3629
                     /locus_tag="KPH93_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1689002.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYANIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3629..4660
                     /locus_tag="KPH93_002191"
     CDS             3629..4660
                     /locus_tag="KPH93_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG1689003.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCSTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4752..5564
                     /locus_tag="KPH93_002192"
     CDS             4752..5564
                     /locus_tag="KPH93_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG1689004.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5567..6487
                     /locus_tag="KPH93_002193"
     CDS             5567..6487
                     /locus_tag="KPH93_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG1689005.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6662..7108
                     /gene="rpiB"
                     /locus_tag="KPH93_002194"
     CDS             6662..7108
                     /gene="rpiB"
                     /locus_tag="KPH93_002194"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG1689006.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7138..7803
                     /locus_tag="KPH93_002195"
     CDS             7138..7803
                     /locus_tag="KPH93_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG1689007.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7913..9415
                     /locus_tag="KPH93_002196"
     CDS             7913..9415
                     /locus_tag="KPH93_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG1689008.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVRKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGSIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEISKKGEI
                     IVKNLIEDYSNLLIDKIRRGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9727..11319)
                     /locus_tag="KPH93_002197"
     CDS             complement(9727..11319)
                     /locus_tag="KPH93_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1689009.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKESALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILILMLLALRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNPKDMSITYVFFSNLLSMYNRKTINKKLAIVFLISIVIQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEESIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     ANMEIIIDADLFKVMLTFKRVN"
     gene            complement(11288..12004)
                     /locus_tag="KPH93_002198"
     CDS             complement(11288..12004)
                     /locus_tag="KPH93_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689010.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILLE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNSSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12554..13354)
                     /locus_tag="KPH93_002199"
     CDS             complement(12554..13354)
                     /locus_tag="KPH93_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG1689011.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNNVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRKNIILLPEINTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFTDKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEHREGIGYKSDHKSFDKLLSNKDVPAILHTPNL"
BASE COUNT         5268 a         1569 c         2246 g         4317 t
ORIGIN      
        1 cataataaat aattttataa aatagttgtt aaacataaaa aaccatttgt aaaaaacaaa
       61 aaaatcattt ctctaacaaa aaaaaccaca aaaaatataa aaaagtatgg ctagttaagt
      121 gattaacaag taaaaattaa aataaaaatc aaaaaaaatt tatcaatgaa agtcagctaa
      181 aatcctagaa gagagcatta tttttgggaa aacattttct tttgcttaac aaaaaaagcc
      241 actttttggc acacaacatg ctttatataa agtcaagaaa aactaagata taagacaaag
      301 gcaattagga ggtgtaaata tggagtttat aaattttgta agtgataaag ttatagacct
      361 taaaatggat tgtaaaacga aagaagattt ctttaaagaa atacataata aagtttttga
      421 tttaggcttt gtaaaagaag aatttggaga aaaaatatta gcaagagaaa atgtatttcc
      481 aacaggttta aatctaggcg attatggagt agccatacca catacagatg cagaatatat
      541 taaagagcaa tttatagcag tctgtacttt taatgaacca gtagtattta gttctatgga
      601 agaccaagat gaaaaagtac ctgtaaattt ggctttcgta ttaggactta atcaaccaca
      661 cagtcaatta tctgtattaa cagaattaat gggaattatg caaaataaag agttagttga
      721 taagttgatg agttctactg ataaagaaga agttttaaag acactaaagt ctttataaaa
      781 ataaaaaagt actaaaaata aaaaatataa aaataaaaat tattaggagg aaataaaaat
      841 gaaaaaaata ttagtagcat gtggagcagg aatagcaaca tcaacagtag tttgtgacaa
      901 ggtagagaga ttagttaagg aaaataatat acaagcagaa gttatacaat gcaaaatagc
      961 tgaatgtgca acaaagcaag aaggagcaga tttaatagta tcaacaacta tactaccaac
     1021 aacttataat ataccagcaa taaaagcaac agcatatata actggagtaa atacagcagc
     1081 attagataaa aaaatattag atgctttaaa ataatcctat catagataca ttgaaataat
     1141 attttataaa acagaaaaag gaggatttat aaatggataa attattagca gtagtacaat
     1201 acattttaaa tatgggaccg acagtaatac taccaataac aattgtagta ctaggaatga
     1261 tatttagaat taagtttaca aaagctttaa agtcaggtct tacaattggt ataggatttg
     1321 tgggtataaa cttagttgta aacctattaa catcaacact agggccagcg gctcaaggaa
     1381 tggttgaaag attcggactt aatttaacag taatagatgt tggttggcca acaacagcat
     1441 cagcaacatg ggcttcacca gttgcaccaa tattaatacc agtttgttta ttagtaaact
     1501 tactattatt atacttcaat aaaacaaaga tattaaacgt tgatatatgg aattattggc
     1561 actttatagt agcaggtgca gttggatata tagtaacagg aagttatgtt tgggctatat
     1621 tctgtgcgat attaatggaa ttattagtac tatttattgc agaaaagaca gcaaaaaatg
     1681 ttcaggagtt ttatgattta gaaggtatag cattaccaac tggttcaaca gcttcatttg
     1741 ctccacttgg atatttagta ggtaaatttg ttgaaaaaat accaggaata aataaaatac
     1801 aagcagaccc agactctata caaaaaagat ttggtatatt tggagaacca atgatgatgg
     1861 ggattatact aggtataata cttggagcac ttgctggata tgatgtagca ggaatattca
     1921 atgtaggtat atcaatgggt gcagtaatgt tacttatgcc tagaatggtt agaatattaa
     1981 tggaaggttt aattccaatt tcagaatcaa taagagattt cttacaagca aaatatggag
     2041 acagagaact ttatataggt ctggatgcag cagtagcaac tggacatcca gcagttatat
     2101 caacagctct tatacttgtt cctataactt tattcttagc agttatacta ccaggaaaca
     2161 aagttttacc atttggagat ttagctacaa taccatttta tatggcattt atagtgtgct
     2221 ttagaaaagg taatatagtt caatcagtta taacaggaac aatagtaata gctatatcac
     2281 tttatatggc cacaaacttt gcagatgttc atacacaatt attacaacaa gcaaatatga
     2341 tgccagaagg aacaacaatg gttacaagta tagatacagg tggtaactta ctaaaatggg
     2401 gaatattgaa attatctcaa ttagtttctt catttggaat attctaatat gtagttaata
     2461 gagattactc ttttttagta aagtatgcga aaatttaaca tttagcagct actaaaaaag
     2521 agtaatattt tttataaatt tttaaaaata taaaatacaa gaggaggaga tttatcgtga
     2581 aagcagcagt attacatggc acaaatgata tgagatttga agatatagaa ataaaaccat
     2641 gtgaaagtga tgaagtaaaa ataaaagtta tggcagcagg aatatgtggg tcagatcctc
     2701 caagagtatt aaaacattgg aaatatccag ttccagctat accaggacat gagttttctg
     2761 gagtaattgc tgaagttggt aaggatgtaa aaaatgtaaa ggttggagat agagtagttg
     2821 caattccttt tataccttgc aatgaatgtg aatattgtaa aagaggatta ttttcattat
     2881 gtgatgacca tggaatgtta ggagctaaaa gttttggagc ttttgcagag tatgcaaata
     2941 taaaagctac aaatgtactt ccaataggag atatggattt tgaagatgct gcaatgatag
     3001 agccactagc agttgctatg catggagtac ttaatatagg tgttcaagtt ggagacactg
     3061 tagccgtaat gggtagtgga acaatgggac agttagtaat acaaggactt aaaatagcag
     3121 gagcaggaac aattatagca gttgatatat ctgataataa attaagagaa tcaaaggaat
     3181 taggggctga tataataatc aatgctaaag atattaaccc agtagaaaaa ataaaagaac
     3241 ttactggtgg aaaaggcgtt gacatagctc ttgaatgtgc aggttcaaaa ataactcaag
     3301 aacaatgttt acttataact aaaaagaaaa gtaagatagg atttttaggg atagcatatt
     3361 cagatataac tttatcagaa gaagcatttg aaaatatatt tagaaaagaa ctagaattaa
     3421 aaggtttttg gaactcttat tctgcaccat ttccaggaca agaatggact aagggaatca
     3481 atctagttaa tgaaggaaag ataaaattaa aagaaatggt atctcataga ttctcacttg
     3541 aagatactta caaagcattt gaaatgataa gagatagaaa agaagaattt aacaaaatat
     3601 taattttacc acaaggggtt gaaaaataat gaaagcagta gttaaaacta aaccaggcta
     3661 tgataatgta gaagttttag aagtagaaga accaaaagct acaggagata aggttaaaat
     3721 aaaagtagaa tatagtggta tatgtggttc agatatacat tcatttaaag gtgaatatgc
     3781 aaatataaaa gccccagtaa cattaggtca tgaattttct ggaattgttg tagaagtagg
     3841 agaagatgtt aaaaatataa aagtaggaga tagagttaca tcagaaacta cttttgcaac
     3901 ttgtgagaaa tgtatatatt gtgagactaa agattataat ttatgctcaa ctagaaaagg
     3961 tattggtact caagtaaatg gaagttttgc tgagtatgta ctatcaagag aagaaagcat
     4021 acatgtactt cctgaaaatg tatctttatt atcagcagcc cttacagagc cactagcttg
     4081 ttgtgttcat gcagctcttg aaaagactac catagaaagt tctgatacag ttttaataat
     4141 aggaccagga ccaattggct tattattagc tcaagttgtt aaatctcaag gtgctacagt
     4201 tataatgtct ggtgttacaa aagataagga aaggttagaa atagctaaaa atcttggagt
     4261 agatagaact gtaaatgtaa tggaagaaag tttggcagat gtagtgaatg aaatgactga
     4321 tggaaatgga gtaaacaaag gatttgattg ttctggattt atgccagcag ttaacgaagc
     4381 cttaagattg cttagaaaaa aaggtacttt cgtacaagtt ggaatctttg ctaaaaaatt
     4441 aaatgaaatg gaccaagaag caataatcca aagagagtta caatatatag gaagtagatc
     4501 tcaaaagcca agttcttgga ttacagcatt agatttaatg gcaagtggga aagtaaatac
     4561 agaagcttta gttactagaa ctgttggttt agacgaattt aaagaaggta ttacagcttt
     4621 aatgaacgga gaagaaataa aagtagcagt aaaatcttaa aaaaacattt gtattatttt
     4681 aaaaaaaatt gtaaaatcta ctgtaggata aaaaaatgta aggtcttaaa aaataacagg
     4741 aggaagaaag tatgagcact cttagagatc attctaataa tattagaaaa aacataataa
     4801 aaatggttgc agaagctaaa agcggacatc caggtggttc attatcaata gctgatatgt
     4861 tgacagtatt atattttgat aagatgaacg tatgtgcaga aaatccaaaa atggctgata
     4921 gagatagatt tgttttatca aaaggtcatg cagcaccagc tttatatgct acattagcag
     4981 aaaaaggatt tttcccagaa gaagaattaa taacactaag aaaatttggt tctaagttac
     5041 aaggtcaccc agacatgaaa aaagtagcag gaatcgatat gtctactggt tctcttggtc
     5101 aagggctatc tgcagcaaat ggtatggcac ttgcaggaaa attagataac aaagactata
     5161 cagtatatac tatattaggt gatggagaaa tccaagaagg tcaaatatgg gaagctgcaa
     5221 tgacagcatc acattacaaa ttagataatt taattgcatt tgttgactta aatggattac
     5281 aaatagatgg ttctaatgaa gaagtaatga atgtaaatcc agtagatgac aaattcaaat
     5341 cttttggatg gaatgtaata gtaataaatg gacattcttt tgaagaaata ggaaatgcag
     5401 ttgatgaagc taaaaaagtt acaggaaaac caactgtaat aatagctaaa acagtaaaag
     5461 gtaaaggtgt ttcatttatg gaaaataatg ctgcatggca tggaacagct ccaaatgcag
     5521 aacaagcaga gcaagcattg aaagatttag aaggaggtct ataggaatgg gaatagcaac
     5581 tagagaagct tacggacaag tgttaaaaga acttgcagaa aataaagata tagtagtatt
     5641 agatgcagat ttaggaaaag ctacaaaaag tatatcattt aaagaagttg ccccagatag
     5701 attttttgat atggggatag cagaaggtga catgattggt acagcagcag gccttgctac
     5761 ttgtggaaaa ataccttttg cgagtacttt tgcaatattt gcagcaggaa gaggctatga
     5821 gcaaataaga aactctgtag catatccaaa tttaaatgta aaaatagcag ctactcatgc
     5881 aggtgtaaca gtaggtgaag atggtggtag ccatcaagct atagaggata tatctctaat
     5941 gagaggtata ccaaatatgg ttgtattaaa tccagcagat gctttagaag caagacaagc
     6001 tattcttgca tctattgatt ataatggacc tgtttatata agattaggta gagctgcaac
     6061 tcctgatgta aacagtgaaa attataaatt tgaaatagga aaaggaactg ttttaagaga
     6121 aggttctgac ataacagtaa tcgctacagg aataatggta gcaaaagcct tagaagcagc
     6181 agaagaactt gctaaagaag gtataaatgt agaagttgtt aatatatcta caataaaacc
     6241 attagatgaa gcattaataa aagaaactgc taagaaaact ggaaaagtag ttacagcaga
     6301 ggaacatagt ataataggtg gtttaggttc agctgtatgt gaagctttag cagaaactaa
     6361 agatgtagta gttagaagaa ttggtgttaa agatgtattt ggtcaatcag gtacaccagc
     6421 tgatttatta aaacactatg gtttaactac agaagatata gtaaaaaata taaaagagtt
     6481 gttatagata taaatatatc taaataacaa attgatttga cataagtcaa ttaaagctgt
     6541 cttaaaaata gaaagaaatt tctacaatgg gacagcttta tttttacaat actttatttt
     6601 tatgataatt ataaaatagt atgaaagtaa taaaaaaaat tataaaaaga ggtgctattt
     6661 aatgaaaata tctattggat gtgaccatgg tggttatgaa ttaaaggaat atataaagga
     6721 aaatttaata aataaaggca tagaagttga agatgtgggt acaaattcaa aagaaagtgt
     6781 agattttcca atatatgcaa aaaaggtaag tcaaaatgtt caaagtaaaa aatctgattt
     6841 aggtatactt tgctgtggaa caggaatagg tatgtctatt gcagcaaata agtttaaagg
     6901 aatcagagca gctgttgttt ctgactgttt ttcagctaag gcaactagag aacacaataa
     6961 tacaaatgta ctttgtttag gagaaagagt tattgggaag ggattagcaa tgagaattgt
     7021 tgaagaatgg ttaaattcaa gttataatgg agaaagacat gcaagaagat tacaaatgat
     7081 tgatgatata gaaaaagaaa atttataaat atataattta taacaagggg gaacgttatg
     7141 tctattattt gtgcttcaat aatgtgtgca gaccaaatga agttaaaaga ggaattagaa
     7201 gctcttgaag aagctggtgt aaaattactt cattgtgatg ttatggatgg aatatttgtt
     7261 aaaaacttgg ctatgggacc tgagttattg aaatcaataa gtgaaaatac aacaatacct
     7321 ttagacatac acttagccac agaaactcca gataaatata ttgatatgat gtcttatata
     7381 aagccaaaat atatttcttt tcatgtagaa tcaagtacaa atgtaaaagc tgatatacaa
     7441 aagttaagaa attatggtat agggccagtg ttagcaataa gccctcaaac aagtgtggat
     7501 aaaatagaag aatatataag tttagttgag ggtatcttag taatgacagt aaatccaggg
     7561 tttgcaggtc aaaagtttaa tctttctgta ttggataagt tggataaact tacagagata
     7621 ctaaaagatt atgataatcc tccatttata gaggttgatg gaaatataaa taaggatact
     7681 attaaactta tgaatggtaa aaaagtagat atatatgttg ttggtacttc agctttattt
     7741 aatgacaagc ctccaatttc atataaagat aaaatagaag aactaaaaga aagtataaag
     7801 taaccatgta ataaaattat aatatatttc tgtttaatcg taaaagttta gctagaaaca
     7861 agaaatttta taatatttaa taaaaatttt gattgagtaa ggagaatttt ttatgggaga
     7921 cctatataaa ctgcaaaatg gtactgatat tagaggaata gcgtatgaaa atgattctaa
     7981 agaagttaac ctaactgtag aagaagtaag aaaaatagct aaagcgtttc atatttggct
     8041 aaaagaaaag actaagaaag ataaagtaac tgttgctata ggaactgatt ctagaataac
     8101 aggaagccaa tttagaagta ctgttattga aactttaact aatgatgatt gtaatgtaat
     8161 tgattgtgag attgccacaa ctccagctat gtttatgaca actattatgg atggatataa
     8221 ttctgatggg tcgattatga ttacagcaag tcacttacct tattattata atggtttaaa
     8281 gtttttcaca gaaagtggag gcttagaaaa aactgatata aaagagatgt tggatatagc
     8341 tgttaaaaat gatagtgtag actatgagga aataagtaaa aaaggtgaaa ttatagtaaa
     8401 aaatttaata gaagattatt ctaatttatt gatagataaa attagaagag gtgtaaattc
     8461 aagtaaaaat tatgaaaagc ctttttctgg gcttaagata ttggttgatg caggaaatgg
     8521 agctggtgga ttctttgctg aaaaagtttt acacatactg ggagcagata cgacaggcag
     8581 tcagtttttg aacccagatg gtatgttccc aaatcatata cctaatcccg aaaacaaaga
     8641 agcaatggaa agcatatgta aagctgtgtt ggacaataag agcgacctag gaataatctt
     8701 tgatactgat gtagacagag ctgctattgt aggtaaaaat ggcaaaccaa taaataaaaa
     8761 tgctcttata gctgttatat catcaatagt attagaagaa catccaaaaa cagctatagt
     8821 aacagattcg attacttctg aaggacttgc taaatttata aatgaattac aaggaagaca
     8881 tcatagattt aaaagaggat ataaaaatgt aataaatgaa gctataagat tgaatagtga
     8941 aggtgaagag tgccatttag ctatagaaac gtctggacat gctgctttaa aagaaaatta
     9001 tttcttagat gatggggcat atttaatagc taaaatttta ataaaagtag caaagttatc
     9061 cttggaagga aagactatag aagaattgat agaaaacctt gaagaagctc aagaagagaa
     9121 agaagttaga ataggcatta ataaagaaga ttttagacca tatgcagagg atatattaaa
     9181 agatttagat tctcatgtta agagtattga tggatggtct gtagcaccaa ataattttga
     9241 aggcataaga gttaattgtg atgaatcaaa tggagatgga tggtttttac ttagaatatc
     9301 actacatgaa ccattattag ctttaaatat tgaatcaaat aaaattggtg gagcagaaca
     9361 aatttattct aagttaaaac ttttcttaga aaaatatgat ttaaaaggtc tttagggtaa
     9421 atagaaagaa tatatttata ttgaagaata aatgtaaatt tatacctaag attacaaata
     9481 ttacaaaatt gtaatcttag gtaacagaga tgtaactgat tatggaaaca ctatgtgata
     9541 ttatagatac agagagatga aatatgtgat gaattataga gaaatataat ttaaaatgta
     9601 ttaatatcac accaaaaagg tataatcaaa aaatatgtaa atataaaccc caacatttag
     9661 aaaaagacct ctaaggaggt ttttttcttg tttaaataac ttaaaatata tttttatatt
     9721 aatatatcaa ttaactcttt taaaagtaag cattacctta aataaatcag catctataat
     9781 tatttccatg tttgcatttt ctaattcaat tatattttga gcaattgata aaccaagacc
     9841 agagccatct gtttttctag atatatcacc ccttctaaat ctttctatta actcttctgg
     9901 ctttagattt agtttatcat ttgaaatatt tttaaacgtt aatattatac tttcttcctt
     9961 tataaacata tctatataga ctctagtgtt aggcatagag tattttagta tattgcaaat
    10021 tagattctca aaaactctcc aagttttttt tccatctatc atggtaaaaa cagcatattc
    10081 aggtgtttct attataaaat ctaaactgct attttgaatt ttgtcttcaa actctacaat
    10141 actttgaatt aaaagttctc ttatatcggt ttttgttata ttaaattcta catctccact
    10201 acttagctta gatgcttcaa atatgtcatc tattaataat tttaatcttt gtgatttttt
    10261 atctaaaata tttatatagt catttatagt ctctctgtca aaatcattat tttttaacat
    10321 atcaacatag cttaatatag atgtaagtgg agtttttaag tcatgagata cgtttgtgat
    10381 aagttctgcc ttcatccttt cattttttat attagcatct acagcagttt caagaccacc
    10441 tgttatatta tttatacttt ttgctaaagt agatatttca tcatccccta taacttctaa
    10501 tttatacttt aaattgccat tttctatatt ttttatattt tttgatatgt tatttatgta
    10561 attaaactta acagataaaa agttgtatat ataaaaatga aaaactgtgt ttacaagtgt
    10621 aaataaaatt actatagcta ttgcctcagc ataatatata tacataaaga atatcaaaac
    10681 cagatattgg ataatagctt gtataactat agatatcaaa aatactatag ctaatttttt
    10741 atttatagtt tttctattat acatactaag taagtttgaa aagaatacat atgttataga
    10801 catatctttt ggattatagt tattatattt aatattttca tatattatgt agactataga
    10861 tataattata aaattgtaga ttattaaatt agtgttataa aacattttat tacttgtaaa
    10921 tgcgtgaaac cttaaggcta atagcatcaa aattaatata agtgtaagtt ctatttttac
    10981 cttagaaaaa aggcttatga cattctctaa gaatttgact ttggtctgtt tatttttttt
    11041 atacaatgta aaacatagta tagtcaataa aaaacttgat aatataatac ctatgcagac
    11101 aaactctaat tctacttttg acaaatgtac aggagtaccg aaaaaataaa aataaggtag
    11161 agcagactct ttataataat aattaatatt ataagaaaag ttattattca aatcataaac
    11221 tgcttcaaat aatatagaaa ctaatagaaa cagtaataat aataaaataa attttgcttt
    11281 agaatcatta ataattaagt ttttcaattt tgtaaccaat gccccacacc acctttaaat
    11341 attttggttc ttttggatta atttcaattt tttctctaat tcttctaata tgaacagcaa
    11401 tagttctctc acaactataa gcatcctcat tccagacatt ttcataaatc tcacgagaag
    11461 agaaaatttt accttttcgt gacataagaa gtgtaagtat tttatattca agtggtgtca
    11521 ttttaatttc ctcaccatct aaagaaacta tttttgtatc tgtatttagt tctagtccac
    11581 cactttttag aatataatta gtatcatttg agctatttgt attactaaaa tcacctaaat
    11641 ttatatacct tctaagttga gattttactc gagcagtaag ttctagtgga ttaaatggtt
    11701 tagttatata gtcatcagca cccatattaa gacctataat tttgtccata tcttctgatt
    11761 ttgcagataa gagaattatt ggtatattcc ttttttctct tattttaagt gtagttttaa
    11821 gcccatctaa attgggcatc attacatcca ttattattaa cttaatatca ttttcgagta
    11881 gtatgtctaa tgcttcaagt ccatcatatg cctttaaaac attaaagtta tcaggtctca
    11941 aatagaactc tatagcatca actatctctc tatcatcatc tacaaccagt atattttgat
    12001 tcaatttatt acccccttga aattttattt ttaaccaatt tttattttat accaaaaaga
    12061 ggtattagaa aacagtaagc gtattcacta tgaaataaaa actagtattt ttaatatatt
    12121 gtcaatgcat atagcaaatt taaaagtata gacatccata ttaatctgaa gttaaaagtt
    12181 tatgaaaata tctaggataa tttatcacac tccttatgtt attttgggtt tattaatttt
    12241 ttaggtctaa acatatatta acaaatataa ataacaaatg agtttcataa ttattaagaa
    12301 tttataacaa atatttattt taatgtagtt taatgtagtt taatgtaaat aggtagttta
    12361 atgtagatat gtataaagca tttaaaaagg cttataggtt ttaatgatga tacttttata
    12421 tattttaaga gatgatgaaa ttagatatga gtaatagttg agtgagaaaa agtagtatag
    12481 cacaaaaagt gctatatcac agaatctcaa ataatttcct tcttataact taaactaaaa
    12541 gaataaatat ttcttataaa ttaggtgtat gaagtatagc aggaacatct ttattactta
    12601 ataacttatc aaatgattta tggtcagact tatagccaat accttctcta tgctctaatt
    12661 catcaacaat tttgtattgt tgacaggcat ttttaacctt ttcatagtct tcagtagaat
    12721 gagctttata aactatttta ttattaaaag ttattttatc agtaaattta ggctttacaa
    12781 aatatttagc atcttttatt ttaccagcat tatataaatt ttggataaca gaaccatttt
    12841 ttaaatgttg taaatggtca tctaacttat aagtatgtgc tatatgagtt acatttttaa
    12901 atttttcttc aaatgtaaga gcaatttttt ccatgtaatt gaagtcagta ttaccatttg
    12961 tatttatttc tggtaataat attatatttt tccttattac accaaggcta ttaagagaag
    13021 ctaaaaattc tctatttcta tattctgatt tttgtatatt tgttaggttt tcatattttt
    13081 tatatttatc aaagacagat ataccacatc cagtagatac taaaaccaca aatacattat
    13141 tttttcctct ttcttttata gcattaacaa tagcactccc agcccacaac acttcatcat
    13201 cttgatgttg aacataaaag acaacataat ctttaaattt tttatgacta taattcacat
    13261 agctactaaa accggttaaa atcaaacata aaactagaat aatagataga atttcatatt
    13321 tatttatagt aaatttttta ttcttaaatg tcatttaatc atctcctgaa aagttgtctt
    13381 atttatatta tcaaacaaag
//
LOCUS       DAEMAE010000099        13250 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEMAE010000099 DAEMAE010000000
VERSION     DAEMAE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.101"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(113..1069)
                     /locus_tag="KPH93_002200"
     CDS             complement(113..1069)
                     /locus_tag="KPH93_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG1689012.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDR
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1437..2225
                     /locus_tag="KPH93_002201"
     CDS             1437..2225
                     /locus_tag="KPH93_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1689013.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKVVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2347..2817)
                     /locus_tag="KPH93_002202"
     CDS             complement(2347..2817)
                     /locus_tag="KPH93_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG1689014.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVKEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILDIIKK"
     gene            2956..3600
                     /gene="uppS"
                     /locus_tag="KPH93_002203"
     CDS             2956..3600
                     /gene="uppS"
                     /locus_tag="KPH93_002203"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG1689015.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3643..4548
                     /locus_tag="KPH93_002204"
     CDS             3643..4548
                     /locus_tag="KPH93_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1689016.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDFGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQAVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4862..6409
                     /locus_tag="KPH93_002205"
     CDS             4862..6409
                     /locus_tag="KPH93_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1689017.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHLGVDIKVLILILL
                     TLFSINVFILYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPALGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINISRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVRFISERLYEIKSL
                     GMKISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDETNYKKLLTDGSYINLEDTLLDK
                     CMAVEEE"
     gene            6412..6810
                     /locus_tag="KPH93_002206"
     CDS             6412..6810
                     /locus_tag="KPH93_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG1689018.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6849..8582
                     /locus_tag="KPH93_002207"
     CDS             6849..8582
                     /locus_tag="KPH93_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG1689019.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWIVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGVYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8814..10025
                     /locus_tag="KPH93_002208"
     CDS             8814..10025
                     /locus_tag="KPH93_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG1689020.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMEIVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10217..10477
                     /locus_tag="KPH93_002209"
     CDS             10217..10477
                     /locus_tag="KPH93_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG1689021.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10830..12542
                     /gene="ptsP"
                     /locus_tag="KPH93_002210"
     CDS             10830..12542
                     /gene="ptsP"
                     /locus_tag="KPH93_002210"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG1689022.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHVLGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         5370 a         1268 c         2204 g         4408 t
ORIGIN      
        1 ttttttaaaa gtaatttgta ttttaaaata aaaacacacc ccctaaatac aaagtaattt
       61 gcatttaaga ggtatgtatt tatcatcaaa ttattagttt attttggaat ttctaatctt
      121 caaccataat atctttaaat ataaaatcat gcattgcatc tagttcatat tcctcaaaag
      181 gtattacata gcctgcatta cctatttttc taccattttc agtagaaaga ggaaattcca
      241 tagatttcaa atttaaattt cctatactta aaagttcttt tcctaaagat attatttcat
      301 taggattcat attagttttt acatacggaa gtattgtgtt aactagatta ggatatttag
      361 tgactggtaa atccttaaca ccatttatta aactctgtat aacacttctt tgtcttctat
      421 ctctttcatc tgtgctatca ttttttctta tacgagaaaa tgcaagtgtc tgatatccat
      481 ttaatttttg ctcaccagaa tttcttatat atttaagaga acctttattg tcatttttat
      541 tccatttata agtttcagga ataaatttat ttaattctct tatttcactc ctatcaacag
      601 taacagtaac accacctaaa gcatcaacta tatccataaa agagaaaaag tcaactacag
      661 cgtagttttg tatatcaagt tggaagtttt cttctatagt ttctattaat aagttagctt
      721 gtccatatac ataggcatgt gttaattttt gttctccacg tcctggtata ttaacataag
      781 tatctctatt taatgaagtt aatttaagac ttttattttt attatcaata gtaagtatca
      841 tcatggcatc agaacgagaa acttcttctc ctggtcttgc atctgtacct aacaataata
      901 tgtttgtgat attttcttcg tttttatagt cattactact aagcgcctct gtatttatac
      961 tatcatcttg tatagttttt aatttagtaa aaacatatcc atatatacta acaggaaatg
     1021 ctataactaa aagggctaga aatataacga actttttgag tcctgacaat aatatcccct
     1081 actttctaaa ttttttaatt acacatagtt aattatatta aaaatttgtt acataagcaa
     1141 taaaaaacac aataaattag cataagtttg tcataatata attattttta tagtatttta
     1201 tagaagaaag tgtgtagtta ttattgattt tattggaata taattgtatg aagacttaaa
     1261 tttttataaa ctgatgtgta tattgaaaaa aatattttcc tgaatgtagt tatagcatct
     1321 tatgaaaggt gcaaattgta atttaaaatt tttagtgtat aataagaatt gaaaattcaa
     1381 accgataaaa attaataaaa aataattata aattaataat ttgggggagg aaatgtatga
     1441 taaaattaat agcaacagat ttagatggaa ctttgttaga tgaaaagagc gaaataaatc
     1501 cagagtttta taaagtattt aaaaaattaa gagagagagg gataatgttt tctgctgcta
     1561 gtggcaggca gtatcaaaat cttataaaaa aatttgaaga tataaaagat gatatgatgt
     1621 ttatatcaga aaatggaaca ctagtagtat ataaaggaaa agaaatatta tcaaatcctt
     1681 taaataaaga attagtaaat gaaatcatag agacaacaag aagtataaaa gggaaaaaaa
     1741 ttgtaatgag tgggaaaaaa tacgcttata tagaaagcaa agatgaagcg tttatacaag
     1801 aggttagtac ttattatgca aagtttaaag ttgtagaaga tttgacgaaa gtagaaggag
     1861 acatattgaa gatagctgta tttgacttca aaggtgcaga acataataat aatatctact
     1921 ttgaaaagtt ttctgataga gctcaagtat gtatttctgg agtggagtgg cttgatttaa
     1981 ctgcaaaagg tgcaaataaa gggagtgcca taaaaaaagt acaaaaaatg ttagatataa
     2041 aatatgaaga aactatggta tttggtgacc agttaaatga tgtggaaatg atgaaaagtg
     2101 cttatcatag ttatgccatg gaaaatgcaa atgaacattt gaaacaaatt gctagattta
     2161 gagcaaaaag aaatactgaa aatggcgttg tagataaaat aaaagaggtc ataaaaattg
     2221 gatagtcata tgtatagatg aaaaagatta ttttgaagaa atcaaaaaat atcaatgtaa
     2281 ttttgataaa taagtttatt tatgcgaaaa ttacattgat atttaataaa catcaataat
     2341 agatatttat ttttttatga tatccagtat tttgttttct atttgagttg cattttcagc
     2401 agaaattaaa taaacatatc tacctgcctt ctcaattata gtattatttg ctatttcaga
     2461 attgattttt tctccatgtc cttctccaaa aggtgcttgt atcatatctt ttttataatt
     2521 ttctaaagtt ttatagattt tatctacctc gtcagttttt ataacgataa tatcttctag
     2581 agataatttt tcagaagaat taattacaaa accttgaatt atgtcttcct ttacatcatt
     2641 aaaatagtta aagtcctttg catcaaagat ttttgattcc tttatgagca aatcagaaga
     2701 ttttaatgct tttttaattt ttgatgtaga aacgtttttg taatcaccac ttgaagaaca
     2761 actaaccatt cctaatgata agattatcga aaaaaacaat attaaaattt ttttcatgat
     2821 aaacctccaa gttttttaaa ctctgtctta aattagttta ctacataatt atataaaaat
     2881 aaatgtattt aatgttacag ttttgattaa taatgtaata ttttgataat aatacctatt
     2941 gagaggtgat tagaaatgag aataccaaaa catgtaggaa ttatacctga tggaaataga
     3001 aggtgggcaa gtcaaaatgg tatgacaaaa gataaaggat atgaaagtgg aatagaccct
     3061 ggactagagt cttatagatt atgtagagat ttaggctgtg aagaggtaac tttttatgga
     3121 tttacaactg ataatacaag acgtccatca gtgcagacaa aagcttttac aaaagcatgc
     3181 attgatgctg caaatatatt aatagatgaa ggtgcagctt tactagttgt aggaaataca
     3241 aaatctccaa tgtttccaga agaattatta ccatatacta atagaattaa agccaaagaa
     3301 ggtcaaataa aagcaaatct tctagtaaat tatgggtggt attgggactt aaataatata
     3361 tcaggagtag aaaatgtaaa taaaaataat atacaggatt acatacagtc atttgatgta
     3421 tccagactag atttgataat cagatggggt ggaagaagga gattaagtgg tttccttcct
     3481 gtacaatcaa tttatgcaga tttttatgtt atagataatt attggccaga ttttaagagt
     3541 gacgatataa aggacgcatt tagttggtat gatacacaag atgtcacttt aggtggataa
     3601 ttaaaaatat tctgatttaa ggttgtagag tgttttaaat tattgaaaaa aaaacttctt
     3661 gatggtaaaa taacactaat aatatgtaaa agtgtaaaaa tttataccaa gaagggtgaa
     3721 gaaatgtcaa aaggcaataa taacaataat tcaagaaaca aatctaaaaa gaccagtcat
     3781 ctgaatagaa aaaagcgaaa attaaataag aaaaagttag cagtgttgat ttgctttaca
     3841 gttttatttt tatttattgc atttaaggct actcaaggtg tagtagcttt ggtaaaaagt
     3901 atggataaaa gtaataaaac tagtcaacaa caaaatgtta actcagaaca atttgacttt
     3961 ggtaatgaag aggaaaataa aaagaaaaaa tatactgtat ttatagaccc aggtcatgga
     4021 ggcaatgata aaggaactga aagcaagacc agtaatagat atgaaaaaga cctaaatcta
     4081 caaattgcaa aaaaattagc aaataaatta tctaaacaaa aggatattca agtagtagta
     4141 tccagaactg atgatacata tatatcatta aaggataggg ctatcttagc aaataatagt
     4201 tctgctgatg tgcttgtttc tattcattta aatgcagaga aaaatggtaa tacggcaaca
     4261 ggtatagaaa cttggtatag gaataaagct acagatggtt caaaagaact tgcacaggca
     4321 gtacaaagta ctatagtatc ctatgttaaa gttagagata ggggaatagt tgaaaataac
     4381 tttgaagtac ttagagaatc aaatatgcct gctatattga tagagtgtgg ttttttaaca
     4441 acaccttcag aggaacaaaa aataataaat gaaaaatatc aagaccaact tgcagaagga
     4501 attgttcaag gtgtactttc atacctagat tctaaaggga ataagtaagt taactttact
     4561 ttaatgtgag gataaattta aaagtttaac tgtgtaattg tattaacttt atttcgtaat
     4621 gttggaaaca ttgtttgaat ttagaaacta tgaaattaga aattatttta tctatatcta
     4681 atttcatagt tttttttgca aaaaatttat agttataaaa tttaaaaaaa taaatttagt
     4741 ataatgtaat gtagttattt atatgtgatt ttaacttgca ttgatttgct atcaattgtg
     4801 ctttataatt aatatatata aagtttaact gtaagataat ttaaaaaata ggggaattta
     4861 tatgaataaa aatagtatga caaaaattta cagtttaaat aaaataattt ttattataat
     4921 tggaatttta ggaatagttt tacttacaag acatcttggg gtagatatta aagttttaat
     4981 tttaatttta ttaaccttat ttagtataaa tgtatttatt ttatatctta aaattaagtt
     5041 gtatgaggat aaaattaatt taggattgat aaataattca gataagttta taaatattac
     5101 taaagatgag aacttcctaa aacatgttca aaattatatt atcagtaatg aaaaagaaaa
     5161 ctgtgtaatg gcttgttttg atttatgtag actcaagctt ataaatgata tatatggtta
     5221 tgagttaggg gatgaagtgt taaataatat tttaagtaac ttaaaaaatt attttggaaa
     5281 agaagctatt tatggaaagt taaaaagtga tgtttttgta ttaataataa agcttgaaaa
     5341 tagggaacag aagattcctt atttagtaaa tcttattaag aataaaattg tgatactaag
     5401 tcagattgac agttttaaaa tggatataaa tattgaggct tctattggaa tttataaatt
     5461 taataataat gaaattgata taaaaaaagc tatagataat gctgatatgg caaggcttaa
     5521 atcaaaagga ctaaaacaca tagagtatgt agaatttgat aatgctatgg aagaagaaat
     5581 acaatcaatt atgaaaatag agcgagattt attcttggct attaaaaata aagagtttgt
     5641 aattcactat caacctaaag ttgattcaag tacaggtaac attataggtt cagaggcatt
     5701 aataagatgg ttgcaccccg cattaggcat ggttggacca aataagttta tacctatagc
     5761 tgaaaaaaat ggtctgataa acaatattgg tagatggcta attcaagaag tttttattac
     5821 cattaacaag tggatttcag aaggtataaa tgtcttacca gtctcaataa atatttctag
     5881 agtagaacta tataaaaatg atttagttga tttttttaaa ctgatgttta atacatataa
     5941 tattccaaaa gaacttatag aattagaaat tacagaaacg acagcactaa gagatgtaag
     6001 atttataagt gaaaggcttt atgagattaa atctttagga atgaagatat cattagatga
     6061 ttttggagta ggtaattcaa attttatcaa tcttaagggg ataccactag atattataaa
     6121 aattgatagg tcacttatat tggatatagt tacaaataca aaaacaaagt ttatggtgaa
     6181 agcaattgtt aacctttcac atgatttaaa cgtaactgtt atatgtgaag gggtagagga
     6241 tatgcatcag gtcaaagttt taagtgaact gggatgtaat ttcatacaag gttatgtatt
     6301 ttttaaacct ttagatgaga cgaattataa aaaacttctt actgatggaa gttatataaa
     6361 tctggaagat actcttttag ataaatgcat ggcagttgag gaggagtaag gatgatttca
     6421 gaagatgtag attattcaat aagaatcata agagctatat ttaataatgg agaagataaa
     6481 ccaatagatg ctaaaatgat attaaaaagt gaagatatac caatactaac actatacaga
     6541 ctgcttagaa agctttgtgc aaacaatatt cttactttta acaaagaaga tggcaagaca
     6601 tacatgttaa atgtgtcatt ggatacaata agtctaagaa ctattgtaga agtaattgat
     6661 ggaaaaatat atataaataa atgtacgaga tatgtatcta attgcagtaa ttgcttaaaa
     6721 ggttgtaaag taaatcgtga attgctagaa atagaaaaag aaatttataa acttttatcg
     6781 agaaaatcta taagacaaat actagaataa aaattttcag ctatgataat aattggaggt
     6841 tttcggttat ggatgatata tctcaagata attttctact atctaaagag tatgagaata
     6901 gcttagatgt agatactaaa aaagcaagtg gaatatatta tactcctaaa ataattgtag
     6961 attatattgt aaaaaaaact ttaaaaaatc acgatataat taaaaatcca tatccacgaa
     7021 tacttgatat ttcctgtggg tgtggaaatt ttcttttaga agtatatgat attttatatg
     7081 acttatttga agaaaatata tatgaattga aaaagaagta tgatgaaaat tattggattg
     7141 ttgataacat acaccgtcat atattaaact attgtatata cggtgctgat attgatgaaa
     7201 aagctataag tatattaaaa gatagcttaa caaataaaaa ggtagtaaat gatttagatg
     7261 aatctgatat aaagattaat ctattttgtt gtgatagtct taaaaagaaa tggcgatata
     7321 agtttgatta catagtgggt aatccaccct atattggtca taaaaaactt gaaaagaaat
     7381 ataaaaaatt tctcctagaa aaatatagtg aagtttataa ggataaagct gacttatatt
     7441 tttgttttta taaaaagatt atagatattt taaagcaagg agggattggc agtgttataa
     7501 ctccaagata tttcttagaa agtctatcag gtaaagattt aagagaatat attaaatcaa
     7561 atgtcaatgt tcaagaaata gtggattttt taggtgcaaa tatatttaaa aatatagggg
     7621 tatctagttg tattctaact tttgataaga aaaaaacaaa agagacatat atagatgtat
     7681 ttaaaatcaa aaatgaggat atatgtataa ataaatttga aacactggaa gaacttctaa
     7741 agagtagcaa gtttgaacac tttaatataa atcagagatt gttatctgat gagtggatac
     7801 ttgtaaataa agatgatgaa actttttata ataagataca agaaaaatgt aaatacagct
     7861 tggaagatat agcaataagt tttcaaggaa taattacagg gtgtgataaa gcatttattt
     7921 taagtaaaga tgatgtaaag ttgaaccttg tagatgataa atttttaaaa tgttggataa
     7981 aaagtaagaa tataaataaa tatatagtag ataaaagtga atataggcta atatactcaa
     8041 atgatataga taatgagaat acaaataaaa ggatactaga tgaaattata ggggtatata
     8101 aaacaaaatt agaaaacaga agagaatgta aaagtggaat aaggaaatgg tatgaacttc
     8161 aatggggtag agaaaagctt ttctttgaaa gaaaaaagat aatgtatcct tataaatcaa
     8221 atgaaaatag atttgctata gattatgata acaatttttc aagtgcagat gtatattctt
     8281 tttttataaa agaagagtat ttagataaat tttcatatga atatctagtt ggaattttaa
     8341 attccagtgt ttatgataaa tactttaaaa taactgctaa aaaaatgagt aaaaatatat
     8401 atgattacta tcctaacaag gttatgaaga taaggatttt tagggataat aattatgaag
     8461 agatagagaa tttatcaaag caaataatat caattttatt aaataagtct atagataaag
     8521 gaaaagtaga aaaattgcag ataaaaatgg ataacttgat aatggattct ttaggtatat
     8581 aagcagttgt aagtattaaa aaattatact cttttaattg tttatttaaa tttagaaata
     8641 aaaagttagc catataaaaa atattaaaaa tatatacttt tcaatatttt tttgataaaa
     8701 taagaatgaa tttgtgcaat tttatttttc tccaataact aaaaatagtt ttttgaatta
     8761 atatttgctt aagaataaaa atcagaaacg tttttgatat agtaggtgat aatatgaaag
     8821 gattagtttt agaaggtgga ggaacaaaag gagcctacca aataggtgca tataaagctt
     8881 taagagagct tggtatagag tttcaaggag taactggcac atcaattggt gctttaaatg
     8941 gagcatatat aattcaaaat gatattgaag taatggaaga tatttggtta aattatgact
     9001 atactcattt tatgaatatt gatagagaaa cctatgagag gtttaaaaat gtagatttca
     9061 ctactaaaaa tctcaatgca gtgtttgagc ttataaataa agctagaaaa aatgaaggta
     9121 tagatataag cccattaaga caactaatgg agacaacatt aaatgaagac ataataagga
     9181 aatcaaaaaa agactttgga cttgttacgg tctggtggga taaaaagatt aatccatatc
     9241 cattgtatat cgaagatata cctaaaggaa gattgattga ttatttaatt gctagttcca
     9301 gcttgcctat ttttcaaacg aatatgatgg atgataagtt gtatcttgat ggaatgtttt
     9361 gtgataacat gccaatagga ctcttggaag acaaaggtta tgaagatgtt gttgtaatta
     9421 ggttgattga tgattttatt gggaaaatga atctaaatcg acatcaaaat ataaatataa
     9481 aaactataat accatcagaa tatctaggag gttctcttaa taaagataga gataatgtag
     9541 aaaaaaacat tcgactaggt tacttagata ctatgaaagc atatgaaaga tgcactggaa
     9601 ttaagtatta ttttgatgta gagtataaat ataatgaaga atattgtttt gataaattta
     9661 gaagtatgag caatgaaaca atacaatata tctgtaattt attgaatata aaaagagatg
     9721 cttcacttag aactgttatg gaaattgtaa taccaaagct tggtgaggtg cttggattgc
     9781 caaaggattt ttcatataaa gatttgtttt actgtatata tgaaaagaaa cttgaagaaa
     9841 ataatataaa caggataaaa ttatatgatt ttaacaaggt tatagatgtt gtaaatacaa
     9901 atattgattc taatagaaat ttagcttcta attttgaagt tagacctaaa attagtatta
     9961 aaaagaacaa aaacacaaaa ttattgacaa attgtattat aaaagacata aaaaatcaaa
    10021 gttaaataaa aaacaaaaaa aaatttgtaa ttatactcga aaaaagtgta tattagtagt
    10081 ataatatttt tgtagcacta gtgggaacaa aaaatgacca taggacaaaa aagtaccact
    10141 tagtaccatt aaagtaaaaa acaggaaaac aattttaaaa aatataaaat gtaaagttta
    10201 taggaggatt taaaaaatgg aaaaagtagt tagtatcaaa aatgcaagtg gattacatgc
    10261 tagaccagca ggaatgtttg ttaagaaggc agctgaattt aaatcaacag tagaggttgt
    10321 agcaaaagac aaaactgtaa atgcaaaatc aataatggga ataatgagtt taggtttagc
    10381 tcaaggagaa gaattaaagt tagttgtaaa tggagaagat gaagaagtag ctttagctgc
    10441 aatggttgaa ttagttgaaa gtggatttgg agaatagata taataatttt taaaaatttg
    10501 atatttagaa tctaatatta cttaaagtaa agtaacagag aagtattaaa catatacaga
    10561 ccaacatttg tagatattta atttctttgt tactttcatg tttatttaat cagaagatta
    10621 agactattgt taattgggta taaaaataca aatattttag aaaatgaaat aagatatttt
    10681 taatcataaa ttcagagtaa aaatacagta acaaatagtt tataaaattt tataaaaaat
    10741 tataagtaaa taaaactata aaaatgtaaa ttatttatat aaatttatag agtttatgtg
    10801 ctagatgata ttttttagga ggtagggtta tggcttacaa aggaataggt gcttctcctg
    10861 gtgttgcttt agggaaagcg ttagtagttg aacatagtga attagtaatt gaaaaaaaat
    10921 ctatagataa tgttgaagct gagattgcta aattagaaga tgcagttgca gtttctaaag
    10981 aagaattagt aaaagttaag gaaaaggctt ctgaagaatt aggagcagag gaagcagaaa
    11041 tatttgaagc acatttatta gttttagaag acccagaatt aattggttct gcaatagata
    11101 aaattaaaac tgaaagtgta aatgcagagt atgctttaaa tgaaataaaa gagatgtttg
    11161 ttagcatgtt tgagtctatg gacaatgagt acatgaaaga aagagcggca gatataaaag
    11221 atgttacaaa tagaatactt agacatgtat taggaattaa agttgttgat ttatctgctt
    11281 taagcgaaga agttgtatta atagcacatg atttaactcc atcagacaca gcaactatga
    11341 ataaaaaaat ggttttagga tttttaactg atataggtgg aagaacttct catactgcta
    11401 tcatgtctag aactttagaa atagctgcaa tcgttggctt aaatgatata acttcaaaag
    11461 ttaaggatgg agactttgta gttttcaatg gggacacagg tgaagtaatc gtgaatcctg
    11521 atgaagagac tataaataaa tatactgaat taaaagctaa gtatgaagat gagagaaaag
    11581 cattacagct attaaaagga aaaccatctg taactttaga tggaaaacat gtggaattag
    11641 ctggtaacat aggaacacct aatgatatag aaggtctaat aaagaatgat gctgaaggtg
    11701 ttggtcttta tagaacagaa tttttataca tggatagaga ttctttccca acagaagaaa
    11761 tacaatatga agcatacaaa gctgtattag aaggaatgga tggaaagcct atagttataa
    11821 gaacattgga tataggtgga gataaagaat taagttattt aagtatggag cctgaaatga
    11881 atccattctt aggttacaga gcaataagac tttgcctaga tagaaaagat atatttaaga
    11941 ctcaattgag agctttatat agagctagtg ttcatggaag attaagaatc atgtttccaa
    12001 tgatttcatc tttagaagag ttgttacaag ctaaagaagt tgtaaaagaa gttttagcag
    12061 aacttgattc tgaaggtgtg gcttatgcta aagatgtaga aattggtatg atgatagaag
    12121 ttccatctgc tgccgtaata tctgatgtat tggctaaaca tgtagatttc ttctctatag
    12181 gaactaatga tttaattcaa tacacttgtg cagttgatag aatgaatcaa aagataagtt
    12241 atctttacaa tcaatttaat ccagctgttt taagacttat aaagactgtt atagacaatg
    12301 cacataaaga aggaaaatgg gcaggtatgt gtggagaatc tgctggagac caaaaaatga
    12361 taccaatact tttaggtatg ggattagacg agttctctat gtctccaata tcaatacttc
    12421 ctgctagaaa gttaataact tcagttaaag agtcagacat gaaaaaatta gctgatgatg
    12481 tacttaatat gggtactgct gaagaaataa aaagctatat agaaaagact tttaatatat
    12541 aattaatgtt gtatagttac taaaattcat ttttttaaat aacaaaatta tattttacga
    12601 attaaaaagg agatgtttca aaataaaaat ttgaagcatc tcttttttgt aaaagattaa
    12661 taaaaattac ttaatttgtg taatagtcgt tttgtgaaaa agcaaaactg cattgtggct
    12721 atagtaggta tggatgttat tagcaataaa agtactatta aaatgaagat tcattctcaa
    12781 atggtatcaa aagtagttgt gatggagcaa cataataatc ttgtgattta ataatgaaaa
    12841 ttaaaaaaat atagtattga aaaataatta aaagtattta ataatagaat tagggaagaa
    12901 taagtttata agtaaaaata attgattagt agttaataaa ataaatgtga tatccaaata
    12961 aatcctaaat ctaatttgag ttttaatata gatagtctaa agtttgatta ttaaagattt
    13021 aattgtaagt tgttacagtt gatgatattg attagagatt aaagtttaga gtagaaagtt
    13081 accttaaatt taaaaatgca agataaccca atctaaaaat tataatcact cgataggttt
    13141 aaggatttat ttgtttatta taaataggag ggtgtctcaa aatgaacttt ttagtttatg
    13201 aggcactctc ttttatatag aattaaatat attttattat ttcaacaatg
//
LOCUS       DAEMAE010000100        13068 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEMAE010000100 DAEMAE010000000
VERSION     DAEMAE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.102"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            102..983
                     /locus_tag="KPH93_002211"
     CDS             102..983
                     /locus_tag="KPH93_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1689023.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAANDTIDLKETIPYPQIVFNQRSGLRYIVDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1191..2054)
                     /locus_tag="KPH93_002212"
     CDS             complement(1191..2054)
                     /locus_tag="KPH93_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG1689024.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDVDYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2209..4143)
                     /locus_tag="KPH93_002213"
     CDS             complement(2209..4143)
                     /locus_tag="KPH93_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG1689025.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKEVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGTRVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTDNLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAILVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4255..5268)
                     /gene="galE"
                     /locus_tag="KPH93_002214"
     CDS             complement(4255..5268)
                     /gene="galE"
                     /locus_tag="KPH93_002214"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG1689026.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5292..6161)
                     /gene="galU"
                     /locus_tag="KPH93_002215"
     CDS             complement(5292..6161)
                     /gene="galU"
                     /locus_tag="KPH93_002215"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG1689027.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6359..6679)
                     /locus_tag="KPH93_002216"
     CDS             complement(6359..6679)
                     /locus_tag="KPH93_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689028.1"
                     /translation="MSSKYNVWSFNYEFLGLNSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSDLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6669..8660)
                     /locus_tag="KPH93_002217"
     CDS             complement(6669..8660)
                     /locus_tag="KPH93_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689029.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIIIYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKILLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8783..11344)
                     /locus_tag="KPH93_002218"
     CDS             complement(8783..11344)
                     /locus_tag="KPH93_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG1689030.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKDGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDRVEIKAAEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFMDYVIKNKTEISEDTWNKGHSLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEETKEEVDSNSQDIGDVVEDKDITDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEETSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKETKEE
                     KEIKEEKLENEKSVVIPIKKKENSNKKSKNSSKDKYIENKKEINNYISDKEDSLDDEE
                     VVSKKSRLKETIIAVVIVAIVGVGYFITVGNNKKNDKEDIPKSSTQQQANNKLTEEEK
                     KAQAEKEKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTA
                     KETAKEYEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKY
                     YDYKLEELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11622..11956)
                     /gene="rnpB"
                     /locus_tag="KPH93_002219"
     ncRNA           complement(11622..11956)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPH93_002219"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12088..12765
                     /gene="srtB"
                     /locus_tag="KPH93_002220"
     CDS             12088..12765
                     /gene="srtB"
                     /locus_tag="KPH93_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG1689031.1"
                     /translation="MKKLYRIIINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPVKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLV"
     gene            complement(12916..12990)
                     /locus_tag="KPH93_002221"
     tRNA            complement(12916..12990)
                     /locus_tag="KPH93_002221"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12956..12958),aa:Thr,seq:tgt)
BASE COUNT         4022 a         2290 c         1327 g         5429 t
ORIGIN      
        1 tcttactcta taatcatata taataaaaat gtaaaaaata tcttaaaggt taacttctac
       61 aaatatataa ataaaaatat ctatgaaagg atgaacaaaa attgaattta tatcacttac
      121 gctattttgt tacattggca catttagaac attatacaaa agcggcagaa aatttatcta
      181 taacacaacc aagcttaagt catgctattt ctttactaga aaatgaactt ggggtggctc
      241 tatttgaaaa agaaggtaga aatatagtcc ttactaagta tggaaaaata tttttaaaag
      301 atgtggaaaa atcattagaa attttagatt caagtgtaaa atcattaaaa ataactggta
      361 ctggtgaagg acaaatagac ttagcttttc ttagaacact tggtactgac tttatcccag
      421 atatagtgca taaattttta aaatctaatc ctgctaaatc aatagatttt aaatttcata
      481 ctggtgtaac tactgacatt attcaaggtc ttaaagaaag aaaatatgat attgcatttt
      541 gttcgaaact tgaaaaagaa aagggaattg aatttatacc tgttgccaaa caagatttag
      601 tactaattgt accatatagt catccactag ctgcaaatga tactatagat ttaaaagaaa
      661 ccatacctta tcctcaaata gtatttaatc agagaagtgg tcttagatat atagttgatg
      721 atatgtttaa aaaaataaat caacagccta atatagtcta tgaagtggaa gaagaccaag
      781 taattgctgg attagttgca aaaaatttcg gaattgctgt agtcccaaat atgaatatgc
      841 ttagctttac aaaagtaaaa gtaattcaga ttattcaccc aagttgggaa agaaactttt
      901 acttggcatt tataaaagat agatatttgc ctcccgcaat taaaaacttt aaaaactttg
      961 tcattaaaaa cgctcaatta taatttatta ataaaggggt tatttttaaa tatcctcttt
     1021 tattcttttt ataaaataaa aaaatctata cttttaaaag tatagattcc taactgacag
     1081 tcgacatccg agtctcagta attatcgtta tacaacagca actggattca gtccctgaat
     1141 aactaggtta tttatattat atcagatttt ttaaaacttg tcaattgtat ctaacgcatt
     1201 ttttcaagcc atggtccaac aggctctaca ataggaactt ctttaaatcc ccaatttctt
     1261 atatcattca cactcgttgg agtatatatt ttgtttctga cagatttgaa tattttgttt
     1321 ttttctccat attgcataca ctttggatta gcttctatta accctcttct tgctctgcta
     1381 agaggattgc ataaaatatc tgctatttct aaaccttctc tatagtctac atcttctttt
     1441 ttggcaatta taaaaccctt aaccttatct cttaattctt catctatatc taagtatatt
     1501 gttccattat taaatacatc aaaaaacgcc ttttgtactt ttaaatttga gacatcatcc
     1561 cttgactcta taactattcg tgcagattct acagtttcat tatttacaaa cgaataaaaa
     1621 ttctgaagaa catgtataaa agccacaaca tacggttcct ttgaaggtgc ataatatttc
     1681 tttaatctag ctttatctac tgttactgat attatagtac actctagtct agatagaaat
     1741 aaaggcaact catcatagaa taacttaatc tgtgaatcat caaacttctc aaatgctctt
     1801 gattttcttg atatatctgt caaatgtatt cctatgttac tggttccaaa acaatactct
     1861 ttaaatgcat ccatttcttt cctcaaagaa catacataat cgccttgact tactgcatat
     1921 ttatattcaa aaattactcc tgtcaatgta aatgggtctt catgatttat atcagctctt
     1981 ccactttcat caataaacat tatatatttt ttctttataa tcttactcac cctcttaaca
     2041 tatgatgata acatctatat tcattttata tgagttttaa aaatattcca agatttttat
     2101 ataacttaaa cttaatttta tactaaaaaa ttgagatgta aaacaatcaa gcttttattg
     2161 attgaatttc acatctcaat tataattatt tttcaatata tatttttact attttgcaag
     2221 aatatccatt aatttatcca ttactatata agatactccg cctcctattt gataaggagt
     2281 ttcaactttt ttatttgcaa atactctttg ttgctctgat gataatcctg aattatccat
     2341 taaaactatt ggtgaatgta attttacagc taatggtcca gctgataaag catctactaa
     2401 agcatctgat ttgctaacta atatagcctg taaatcagta tctccgtaga atttttctat
     2461 tacttttgca ttagtatcta ctctactatc tccatatatt ctatttcctg aaatatcttc
     2521 agtagttata tcattcattt ttgctattac tgaatcgtta attgcacttg gtccacctat
     2581 aaagtatgca ttttctaatt ttctttctgt tagccattta taagattctt ctgtaattcc
     2641 atctttattg actaatatta taggttgttt ttcttcccca gctttagaag ctacagatag
     2701 agcatctgct tccccatttc cagaaaccat atatgcagtt tttatttctt ctcgatttcc
     2761 aagttcttca gctattcttg tagcagtttg atatctatct tgacctgcta ttctctttgt
     2821 ttctgtattg aaagtagttt ttatttcttg ctctaattta gaacttattg cattttctcc
     2881 acctatcaag attactgttt taggtgctaa taacttcatt tcttcaacag tttcagttgg
     2941 taaattatct gtattagtaa gtagtattgt tgagtcatag ctacttgcta aaggagttgc
     3001 tgttatacca tcagctattg cattaccatt tactaatatt acagtatctg aactatcagc
     3061 attccagttt ccaactgaac ttatcttctt agctgtatca aatctatttg cacctataat
     3121 tgtatcccat gtaggattta tagttccatc ttcattaaaa gtataatctt ttccatctat
     3181 tttttgagaa ccagttagca tctttccatc tttgtctaaa tagtaagact taccatctat
     3241 ttgttgccat cctgtctgca ttattcctgt tttgtcaaag taatacttat taccatctat
     3301 ttcttgccat cctgttactg ataaaccatc agatcctaga tagtatttct ttccattctt
     3361 ttcttgccat cctgtttgca ttactccatc tgagttaaag taataagttt ttccatctat
     3421 tactttccat ccagatactc ttgttccgtc agttcctaaa taatattttt tgttattaat
     3481 tgttttccat ccagtttgca ttactcctga agcatcaaag taatacattt ttccatttac
     3541 ttttaaccaa ctattctttt ggtatgttcc atcagatttt acataatact tcttaccatt
     3601 ttctgttttc catcctgttt gatttagatt tatcttatat tttgctgcta tctttacata
     3661 gctgtcatta ctataatcta taattacatc tgaacctaca ggaacttggt caaataacca
     3721 tctaacctct ttattgtgca ttcttataca tccaccagaa atatgctttc ctatagaact
     3781 ctcattatta tttccgtgta ttccatatgt atctccataa gttcctttta atgctaatcc
     3841 catccatcta tctcctaatg gatttcttgg acttcctcca ggtatattac ctttataata
     3901 tggtctattt tttattttgt taactacttt tgttttccct gttggtgttt ctgacccctt
     3961 tttacctgtt gcaactctaa attcttttac aagtttatta tttacaaaat atcctagagt
     4021 attctttctt gaatttacta taatcacatg tttactggct gcgcttgatt ctggtacgcc
     4081 acatgcaaat actaaaacta aagtcagtgc tagtactaaa tactttttta gacttttttt
     4141 cactataact tccccctctt ttagaaacat tactacatgt gtctaattat aacataaaat
     4201 taccaatacc ttatatttac aaatttgtta catttataca tataaatata tattctattc
     4261 ttcataccca ttaggattca tactttgcca tctccatgaa tcttcacaca tctcttcaag
     4321 ttcatactta gctttccatc caagctcttt ttctgccttt gaagaatctg cataacacat
     4381 ggctacatct ccagcccttc ttcctacaat cttatatggt atttcttttc cacttgcttt
     4441 agaaaatgct tttactaaat caagaacact ataccccttt ccagttccta aattataaac
     4501 aacaagtcct ggattttctt ctaatttttg taatgctttt acatgaccag cagctaaatc
     4561 taatacatgt atatagtctc taactccagt tccatcatgt gttggatagt cattaccata
     4621 aacactcaat tccttcaatt tacctactgc tattttcgtt atataaggca ttagattact
     4681 cggaacccca tttggttctt cacctattct accactttta tgagcaccga ctggattaaa
     4741 gtatcttaaa agagctatat ccaacgactt atctgctttt gatatatcta ctaacatctg
     4801 ctctatcata agtttagttc ttccatatgg attggttaca gaaagtggga aatcctctag
     4861 tataggacaa gtatgtgggt ctccatatac agtagctgaa gaactaaata caaacttttt
     4921 aacaccatat tctctcatta attcaaataa atttaaagta cttattagat tattgctgta
     4981 atactcaatt ggtttttcta ctgattcacc aactgctttt aaagctgcaa agtgtataat
     5041 tgattcaata ttattttctt taaatacaac atgcatttca tctttatttc tgatatcaat
     5101 attataaaat tttactggtt ttttagataa ttccttgatt ctatcaacca ctattgagtt
     5161 gctattactt aagttatcaa ctataacaac ttcataacct gattctaaaa gttctattgc
     5221 tgtatggctt cctatatatc ctgctcctcc agctactaaa actgccattg taattacctc
     5281 ttttctaata tttatttttc acttactttt tttctcatat agtccattaa atcatctttt
     5341 aaattttctc tctttagagc aaagtctatt gttgcctcta aaaaaccaat cttatctcct
     5401 acatcatatc ttctaccttc aaagttataa gcataaatag cctcttgttt tccaagagtt
     5461 tgaagtgcat ctgtcagttg tatctctcca cctttaccag gtgtctgttt ctctaatatg
     5521 ctaaatattg caggagttat tatatatctt ccaagtatag ctatatcaga tggagcttct
     5581 tcaattgcag gtttttcaac catatcttta actttatata ctctatcttc gatatgtttt
     5641 acatccaata taccatactt attagtatct tctttagcta tcttttgaac acctaataca
     5701 gttgttttat actcattata agtatctatt aattgtttta aacacggaac atctgaatct
     5761 actatatcat cacctaataa tacggcaaaa ggctcatcac ctataaaact ttttgcacaa
     5821 taaattgcat gtccaagacc ttttggttct ttttgtctta tataatgaat atttaccata
     5881 tttgatatgt ctcttaccat ttctaacatc tctgtcttac ctttttgctc tagttctaac
     5941 tctaattcaa ctgatttatc aaaatgatct tctatagatt ttttatttct acctgttatt
     6001 ataagaattt cttcaattcc cgattctata gcttcttcta ttatatactg taaagttggt
     6061 ttatctacta taggaagcat ttctttgggc tgtgattttg ttgctggtaa aaatctagtc
     6121 cctagccctg ctgctggtat tattgctttt ttaactgtca tctgcatttt ttcacccttc
     6181 ttcgtaaatt ttctattgta ccaaattaat ataccatatt tatacttttt cttcaaactc
     6241 tatattatcc caatatttaa tgctataaga taatataagg tagatgaaat ttcatctacc
     6301 tttagtttta taagttaata ttgtattgtt tatattttat ctaaaactta tacaggtact
     6361 aaatttcatc taagatgcct tttatatcac tatctatatc ttcatctaga taagaatcta
     6421 aatcttctaa gtcaacttct agttcttgat atgtcatatc aacactgaca tcttcatcta
     6481 tatccatttc tgatattaat tctcctaaaa tactatttcc atcagaatct aaatcactat
     6541 ctatatcttc atctatatag ttttcattta aaatttcatc taaatcaatc tcgtaataaa
     6601 cttcattatt tttttcatcc cctgagttca atcctaggaa ctcatagtta aaactccaaa
     6661 cattgtattt acttgacata ctttattcct cctgtgaaaa acatattttc atctagttta
     6721 ttggttccat aatcaacaga attatatatt ttttcaatta tgtcttttct tctttctttt
     6781 gtactattat ttaatgtcaa ttgtttaaat acatatgtat ttgttattct ctcgaataat
     6841 atttctttta tactatcatc atcaatattt ctatagtttt tccacaataa ctcaaataac
     6901 ttaaacattt ccctactagt ttcatctaca taataaggat acatattttc tactaaatgg
     6961 tttaatcttg atatagcttg ttttaaaaat acatcaagac ttgcactttc actttccttt
     7021 aacattctat aattgtattt cttcttatct tctcctgaaa ttacaaagat gatattatct
     7081 aagctttttt caacttcatt taaataactg cttatattat atgataaatc agacaataga
     7141 tttttaatct gcttaaacac ttgtacatca agtttagtat tatacttttc aaacaaatct
     7201 atttcttgat tttctaatat tcttggcata tatgctgaca ataaatctaa attgtttctt
     7261 tgttcttgta tttgcttata aagtgaattt ataacttcta ttcttatttc atcatttaac
     7321 tctttttcta aaatcttatc aaattcagat ttcatatagt aaatatttcc aaagaaatta
     7381 aatattatca tatcattcat agctttagac caattcttat atatcacttc aatagaattt
     7441 tctttaagta gaggttgctt tgcatatata aatacatctt gcataagttt ttgaaccata
     7501 tttttactta agtttaaaag tattttttga ttattaaatt ttatttccgt ttctacaaaa
     7561 ttagaatttt cgttttcaag ctctttatta attacaatgt cttttatatc actatttttt
     7621 atagatatga tttctttttg aaaagacttt agaatagtca agctatcgaa atcttcttga
     7681 ataaaaaatg gagcatgttt taatgtatat cccactattt cattttctat atcttttaag
     7741 tcatctattt gcataatttc tctatttttg tcatttatat gtaggttaaa tacattttta
     7801 ttatcatata ttattatatc ttctcctaaa ttcaacttag aacaattatc ttttgctgac
     7861 tttacaatag tcttcttaga tttcttattt ataaaaatta attggtcttg atattttact
     7921 aaaagcatgt ttgtgttttt ataatcaaat ccatctatat ttcctgatat cttgcagaat
     7981 actatatcac aatctttagg aagatttcct attttttggt tatctttaac tctctcattt
     8041 aaatcagatg ggactacact atctatattg ttattaaata tagatacaga ccttactata
     8101 atcccctctc tttccatatg aaagtatccc ttcaatgaca ctcttttatc ttctaaaatg
     8161 tatctttcta tatcatcaaa atttatttca aatctatttt tcccactgat aaatataaac
     8221 ttctcattat ctttattaat tccacccata taaaacttat tgtcatctaa acttaatata
     8281 gtcatattat cttttagata aaatataaat tctctttgac ttttgcagat taaatcatat
     8341 tcatccaaag ttattttcat agttaaattt gctgtaacat ttttacttga attttcactt
     8401 tttatgtaaa aaggcaagtt atttccatag acaattattt gagaatcttc taaaactgcc
     8461 tctaattttt ctttattgtc tgaaaaacta tatatatatt ttacacctgt atccttatta
     8521 acttcatctg atttgctgat tattatattc cctagatttg ttatatgaag ctttttatat
     8581 tcttttgagt atctacttat gccactaagc tcaaacccat ctgaaagctc atatatgatt
     8641 ttttctccta atttcaacat accttcacca cctaaacaga attataagta ttttataaag
     8701 gtgaacaaaa tgtctcaaaa taaataatat tttttttaat actttttatt ctgagacata
     8761 gtttctcctt ttttataatg ctctaagcac cttcaactat ttcttcataa agtttatcta
     8821 aatgtttaac atattcacga tatccagatt tgttctttaa ctcacttctt aaatcttcat
     8881 atttttgttt aagttcttca gcactcaaag aaggagctga ttcttttaat tcttctaatt
     8941 tataatcata gtacttatca tacatagcta tatatgtatc aatagatttc ttagctagtt
     9001 cattacattt ttcttgtgct tctccatatt gagaataatc acctattttt attttatagt
     9061 atccgttttc ttgaattatt gttgaagaaa tacctttagc ctcatattct tttgctgttt
     9121 cttttgctgt tttttcaacc ttaagtgaac ctgcataaac agtgtaatat acacctttac
     9181 catctttata tgcttccatt tcttttgctt tcttttcagc ttgtgctttt tcttgagcct
     9241 ctttttcagc ttttgccttt tcttctgctt cttttttctc tttttcagct tgtgcttttt
     9301 tctcttcctc agttaattta ttgttagctt gttgctgagt tgatgacttt ggaatatcct
     9361 ctttatcatt tttcttattg ttgcctactg ttataaaata tcctacacca acaatcgcca
     9421 ctataactac tgctataata gtttctttta atctactctt cttagaaaca acctcttcat
     9481 catctaaaga gtcttcttta tctgatatat aattatttat ttctttctta ttttctatat
     9541 atttgtcctt agaagaattt ttagattttt tattactatt ttctttcttt ttaataggta
     9601 ttactactga tttttcattt tctaattttt cttcttttat ttctttttct tcttttgttt
     9661 cttttgcttt tttatcttct aagtttttac tttctttttc aagcttcgat tcattatttg
     9721 aagatacttt ttcatctaag ttattattta ttggtttttc tatttttaca ctacgttctt
     9781 cttctatatg ttgacttgtt tcttcttgtt ttatttcttt taattcattt tgctctattt
     9841 cttctgattt tacttgatga ttttcaactg gtttatctaa aattatatca ggagaaatat
     9901 catttaaatt ctcttcttct tgttcaactt cttcattgtc ttttttaata aatccaaata
     9961 atttagcctt ctttttactc tttttatttt ctggttcaat tatatcttct ttattgctgt
    10021 cgtactcttt atctgtaata tctttatctt ctacaacgtc acctatatct tgactattac
    10081 tatctacttc ttctttagtc tcttcttcag catcatcttc tattgaaata ctacttctaa
    10141 agcaaatatc tttatctgca ttttttatca tgtaaatgta ttcttcaata tcgatatctt
    10201 tatttcttct agttcttttt gatgcaattt catgattttc taattcacta tagttatctt
    10261 ttattagact atgtccttta ttccatgtat cttcgctaat ttctgtttta tttttaatta
    10321 catagtccat aaattcctta aaattttcag gaccaaattc tccaattata gtcagtctca
    10381 ttgcaccttc tataaggtct ctatttcttc tctttaaaaa ttctaataca accgttgcac
    10441 aagcaaaatc tttaataatt tctttgccaa aaccatatcc ttcaacagaa tgaagttctt
    10501 ctaatccaca taacttctta tattcattgt gtattgtttg agatatactt tcattattta
    10561 agctatttaa cttatctata aacttcgatt ctatatcatt tgattttgat tcatgtttta
    10621 cttttgaatt tttaatttca tttagtagca tgtcattttc tgaattatct ttacctaatt
    10681 gtaaatcctc aatttcagct gcttttattt ctactctgtc ttgttcgtta ttttcttcca
    10741 cattattagg taccacatct ttattgctag ctaatatttt gtttacgcag aatataattt
    10801 ctctttttaa ttctcttttc gcatccgctt gatttttaag aaataaatct ccacattctt
    10861 tcttttctaa acttattttt ctaagagata tattaagaag gtctttatat agatttaaaa
    10921 ccttataatc cttttctaaa cgtcttcttc tctctgatag ttccatttta tcataagcta
    10981 aaaaactatt taaatcattg gcttttagag agtctaatcc atctttcata gccttattta
    11041 catactttct acattttgct gtagttaatt ctaaatcttt ttctaattca tctatttcag
    11101 gtatgtactt atctactatc catttgccaa taagtggttc tgtatttctt gctaatgtct
    11161 tataaaacaa tatattaaat tttaaaagct ctgcatattt tgcttcatta aattctactt
    11221 tttcaaattt cttttccaaa acaccatcta gcttttcatt tattttagac tcttgtttta
    11281 aaaaacttgc tgtgacattt aaaacagtgt ttcttattat attattttca ctcaaaacct
    11341 tcactcctta aatcaattat tttattgtaa tatttacatt gtgcaaagtt gctaatttga
    11401 ttatgatatc taaataatta tttactaaat ttgctacatc ttatatcata tcatattatt
    11461 tacatcattt tactttttct ttttatatct atcttatatt atatcatttt taatatctgt
    11521 actaacttat attacaatat tcctcaaaat tttaaaagta gattagacaa atttacaaat
    11581 aaaaaaacat cagtttaaac tgatgttttt gactatcgaa ataattaagc aagtctataa
    11641 gcggagttct gtattagata atcatctttc taggcctatt gtcaccaata ggctcaagcg
    11701 acctacctac cggagagtac gagcagcact ctttattcct atcttggtct tgctccaggt
    11761 ggggtttaca tagccaacta gtcgcctagc tgctggtgag ctcttacctc gcctttccat
    11821 ccttaccaca atagtggcgg tttatttctg ttgcactatc cttaggatcg ctcccactag
    11881 gcgttaccta gcaccctgct ctatggagct ccgactttcc tcatgtaaga ttacatgcga
    11941 ttatctggct tacttaatat ttattttact tagttatgtt aacacaaatt cttttacatg
    12001 tcaaagtaat attacactaa aaatttaaat ttattcaaaa atagtgtaat atagatatat
    12061 aaactacatt taactcgagg tgatatattg aaaaaattat atagaataat aattaacatt
    12121 atactagtat tagtaatact ttacagtgga tttaatatat actcaaaact taccaaatac
    12181 aatcatgata ctaaaatatc ctcagaactt caaaaaaaag aatataaaaa agaggactta
    12241 tcaaagatta acagtgattt taagttttgg ctttctgttg agaatacaaa tataaattat
    12301 ccagttgtac aatctaaaga taattcttac tatcttgaca aagattttta taaaaaagat
    12361 tctatttctg gtacactgtt tatggattat agaaataaat ctattgatga caaaaatatc
    12421 ataatatatg gacataatat gaaaaataaa actatgttca acaacctaaa taagtttaaa
    12481 gatgctgact tctttaaaaa aaataataaa ataaaaataa ccttgaatgg aaaagaattt
    12541 ttatatgatg ttttttctgc ttatattgta gaatctgatt atgattatct aaaaacaaac
    12601 tttaacaatg aatctgatta tcaaaattac ataaatgata ttacttctaa atcattgtat
    12661 aaatctcctg ttaaggtaaa tagtaatgat aaaatagtta cactttctac atgtacctat
    12721 gaatttgatg atgccagaat ggtgattcat ggtagattgg tttaaaactc acagacacag
    12781 tttaaaacaa taattttaaa ctgtgtcata ttttatacct aataatttat aaattaggct
    12841 actaaaagtg tgtaatacaa aataacaaac acatcaaaca ataaaaaaag actatgctaa
    12901 aaacatagtc tttactggag ctggtgatag gaatcgaacc tacaacctgc tgattacaag
    12961 tcagctgctc taccgttgag ccacaccagc atacctattc tattgtaaat tagtaaaact
    13021 aatttttaaa gttaacactc taaaaactgc acatatatct taatgaat
//
LOCUS       DAEMAE010000101        12889 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEMAE010000101 DAEMAE010000000
VERSION     DAEMAE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.103"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..499
                     /gene="bcp"
                     /locus_tag="KPH93_002222"
     CDS             35..499
                     /gene="bcp"
                     /locus_tag="KPH93_002222"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG1689032.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSISDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDVQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            509..1252
                     /gene="yaaA"
                     /locus_tag="KPH93_002223"
     CDS             509..1252
                     /gene="yaaA"
                     /locus_tag="KPH93_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG1689033.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGVED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGSPAILTYDGIQYKNIEAENFTRQDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGIFKVESTQAKKARGMMAKYIVKNRIRD
                     IEELKKFNLEGYKYQENLSNNSEYIFVKE"
     gene            1380..4037
                     /locus_tag="KPH93_002224"
     CDS             1380..4037
                     /locus_tag="KPH93_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG1689034.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAYLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKGALRVLGFAKREISSIPK
                     EDGENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSSALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSALLIAVILFVPVFRNAFNLTILNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4091..4193
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4303..5568
                     /locus_tag="KPH93_002225"
     CDS             4303..5568
                     /locus_tag="KPH93_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG1689035.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSKG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENAEDLIADLNQALNF"
     gene            5598..6488
                     /gene="metA"
                     /locus_tag="KPH93_002226"
     CDS             5598..6488
                     /gene="metA"
                     /locus_tag="KPH93_002226"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG1689036.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKCEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKNSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6977..7420
                     /locus_tag="KPH93_002227"
     CDS             6977..7420
                     /locus_tag="KPH93_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1689037.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7451..9268
                     /gene="ftsH"
                     /locus_tag="KPH93_002228"
     CDS             7451..9268
                     /gene="ftsH"
                     /locus_tag="KPH93_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG1689038.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9446..12145
                     /locus_tag="KPH93_002229"
     CDS             9446..12145
                     /locus_tag="KPH93_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG1689039.1"
                     /translation="MDRPNPDILLKKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRISKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDMVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKYNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDAQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKEN"
     gene            12182..12880
                     /locus_tag="KPH93_002230"
     CDS             12182..12880
                     /locus_tag="KPH93_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689040.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDIEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         5052 a         1503 c         2374 g         3960 t
ORIGIN      
        1 aaaattatta ataaaaacaa aaggagagat atttatgttg agtataggaa caaaagcacc
       61 agaatttaca ctagaagata aagatggaaa taaggtaagt atttcagatt ttaaaggtaa
      121 aaaagtagta gtatattttt atcctaaaga taatacacca ggatgtacac gtcaagcttg
      181 tgcttttaga aatgcatatg atggatttaa aaaggaagat gtacaagtta ttgggataag
      241 taaagatagt ataaaatcac atcaaaaatt tgcagaaaag catgaacttc cttttatttt
      301 actttctgac cctgacttag ttgctataaa agcttttgat gtatggaaag agaaaaaaat
      361 gtatggaaaa actgcattgg gagttgtacg tgcgacatac atcatagatg aaaatggaat
      421 tattgaaaaa gtctttgaaa aagcaaaacc agacacaaat gcacaagaga ttcttgaata
      481 tttggaaaaa caggagtaat aagaatttat gattataatg ttatcaccag ctaaaaatat
      541 gaagaatata gaagtatttg atagagattt gagtttgcct tgttttatag ataacacaaa
      601 agaaatagtg gaaaatataa agacatttgg tgtagaagat tttaaaaata aaatgaaaat
      661 taatgaaaaa ttagctgtat taaataaaaa tagatttgaa agcattaagt tcgatagatt
      721 aggaagccct gcaatcttaa cttatgatgg aatacaatat aaaaatatag aagcagaaaa
      781 ttttacaagg caagatgaag agtttgcaaa tagctgtatt cgaatcataa gtggattgta
      841 tggggttgta aaaccatatg atagtatata tgaatataga ttagaaatgc agacaaaatt
      901 acgagtaggt gagtttaaga atttatacga atattgggga aatcgtatat ataaagaact
      961 aatcaaagaa aaaacagcta tcgtcaatct ttcatcaaat gaatacagta agtctataga
     1021 aaaatttatt aaagatagtg atacatatat tacatgtact tttaaagtaa ataaaaatgg
     1081 tatatttaaa gtagaatcta cacaagcaaa gaaagctaga ggtatgatgg caaaatacat
     1141 tgtaaaaaat aggataagag atattgaaga actgaaaaag tttaatttag aagggtataa
     1201 gtatcaagaa aacttatcta acaatagtga atacatattt gtaaaagagt agaagttttg
     1261 ttaaatatta aatgacattg ttaaatttag gtaaaatata cttagtgtgt attgaattga
     1321 acttgatttt aaaatataat tattcttaaa tcaaaaaaag aaaggagtgg tcttccaaaa
     1381 tggaaaatca caaacagatg ccttggcatt caagagcaac gcatgaaatt ttagaaagtc
     1441 tagattcatc agaggagggg ctcaccgata ccgaggcgga aaaacggctt aaagagaatg
     1501 gtttaaatga acttagaaaa aaaccaccta gaacaacttt acaaatgctt tggagtcaaa
     1561 ttacagatgc tatggttatg atactaatag gagcagcaat attatctttg atatttggcg
     1621 aatttacaga agcttttgta atactaataa ttgtaacagt aaatgcagtt attggaattg
     1681 ttcaagagaa aaaagcagaa tcatcattag aagcattaaa aaatatgaac tcacctacag
     1741 ctcgtgttat gcgtgatggt gaagaaaatg ttattccagc aagtaatctt gtagtaggag
     1801 atattgtttt tttagaagat ggagcgatgg ttccagcaga ccttcgttta atagaaacat
     1861 cgagtttaaa aattcaagag gcttctttaa caggagaatc tgttccatca gaaaaagatg
     1921 cagatacaat cttacctaaa gaatgtgtat taggagaccg tagcaatatg gcgtatacat
     1981 cttcaatagt aacctatggt agaggtgtag gtgttgtagt agcaactggt atggatacag
     2041 aagtgggtaa catagcatat cttttagatt cacaagatga ttttgatact cctataaaac
     2101 gtaaacttaa tactgttgga aagacattaa gtgtcatagg tattattgta tgcgttgtta
     2161 tatttgcaat aggtgcattt tatcagagac ctctcttgcc aatgtttatg attgcaattt
     2221 cccttgccat atcaattatt ccagagggat tacctgcaac agctactatt gtaatggctt
     2281 taggcgtaca gcgtatggct aaaagaaatg ctttaattag aaaattacct gctgttgaaa
     2341 cattaggagg agctacagta atatgttgtg ataaaactgg tacattaacc ttaaataaaa
     2401 tgactgttac gcatattgct gtcaatggag attttgaatc tggaaaagtg acatcaatag
     2461 atactgcatc tgaaaaacat ccattagtgt ataaagagat tgtttatgct ggtgcacttt
     2521 gtaatgatgc atgtattaat ccagataaaa ctgatgaaat attaggtgac ccaacagagg
     2581 gcgcgttaat ttttatggcc aaaaaatttg gaatagatca agaagctctt gaagaagaat
     2641 atccacgtgt atttgaacag ccttttgatt ctgatagaaa atgcatgaca actgttcata
     2701 atatagatga aaagattatt gcttatacta aaggagcagt agacgaaatt cttgacctat
     2761 gtactaaaat attaacttca aaaggtgaaa gaaacattac tgaagtggat aaaaagaata
     2821 tacacgaatt atgtcttaac atgtctaaag gtgcattgcg tgtacttggt tttgctaagc
     2881 gtgagatttc atctatacca aaagaagatg gtgaaaatat agaatatgat ttgacattta
     2941 taggtatggt tggtatgatt gacccaccaa gaactgaggt aattgatgca gtaagtactt
     3001 gtaaagaagc aggtataaga accataatga taacaggtga ccataaagtt actgccacaa
     3061 caattgctca tgagttagga atatggagtg aagaaaatac tgtgatatct ggtgatgaac
     3121 ttgataattt gagtgatgat gaactggatg aagctgttaa aaatactaca gtatttgctc
     3181 gtgtgtcccc atttgataaa cttagaatta tacaatctct aaaaagaatt ggagaagtac
     3241 cagcaatgac tggagatggt gtaaacgatt cttctgcctt aaagtctgct gatattggta
     3301 tagctatggg tataagtggt acagatgttg caaaagattc ttctgacatg attttaatgg
     3361 acgatagctt tacaacaatt gcttatgcaa taaaagaagg tagacgtgta tatagaaaca
     3421 tacaaaaagt tatacaattt ttgcttgttg gtaatatagc agagatactt acgttgtttg
     3481 tagcaatatt attaaattgg gatactccgc ttcttgctgt tcatattcta tgggtaaacc
     3541 ttgcaactgc tacgcttcca gcccttgctc ttggagttga cccaccaagc agaaatataa
     3601 tgaagcacca gccagtgaaa tcaaccactt tgtttgaaaa aaatcttata caaagagttg
     3661 ttacccaagg tatatttgtg gcaataatga ctattttagc atataaaatt ggtcttaata
     3721 tagaaaatca ttctgtaggt caaacaatgg cattttgtgt attagcattt tcacagatgt
     3781 tacgagcatt tagtcagcgt tcaaatacag attcaatgtt taatagaaat aatggtaaaa
     3841 atccatttct tttaatttca tttttaactt ctgcgttgtt aatagcagta atactgtttg
     3901 tacctgtttt tagaaatgct tttaacttaa caattttaaa taacttagag tggcttataa
     3961 cgattgtttt agccataatg tctgtaattc aagtagaggt tgggaaaatt attaaaagaa
     4021 aaaaattaaa aaaatagttg acaaaattag gaaaaatata taagatataa aacatagaaa
     4081 aattaaataa atcttatcca gagaggtgga gggactggcc ctatgaaacc cggcaaccag
     4141 tatatttata tacattggtg ctaaatcctg cggcgaaagc tgagagatga gtataattaa
     4201 gcttatacag agcctgagtt atatactcag gctttattta ttttaactct catctgataa
     4261 atagtttaat tttttttact aaaatctaag ggggatacaa gaatgtataa taaagaaaca
     4321 atatgtgtgc aaggaaatta taaaccaggt aatggagaac caagagtatt acctttatat
     4381 caaagtacaa cttttaaata tagcagtata gaccaacttg ctgaattatt tgatttaaag
     4441 gttgatggac atatatattc aagaataagc aatcctacta ttcaagcttt tgaagaaaaa
     4501 ataagtttac tagagggtgg agtatcttct gtagctgtat catcagggca atccgcaaat
     4561 atgttggcag ttttaaatat atgtaaatca ggagatagta tactttgttc ctcaaaagta
     4621 tatggaggaa cattcaattt actaggacct agtcttaaaa aatttggtat agatttaata
     4681 tcgtttgact tagattcaag tgaagatgag atagtagaac ttgcaaagga aaatactaag
     4741 gttgtgtttg cagaaacact tgcaaatcca actcttgaag tcatagattt tgaaaaaata
     4801 gcaaatgtag ctaagagaat taatgttcca tttattgttg ataattcatt agcatctcca
     4861 gtgctttgca accctttaaa gtatggagca aatatagtta ctcattctac cacaaaatat
     4921 ttagatgggc atgcttcaag tgttggagga attatagtgg atggtggaaa ctttaattgg
     4981 gataatggaa aatttccaga attagttgag ccagacccaa catatcatgg aataagctat
     5041 actcaaaaat ttggaaatgc tgcatatgca actaaagcaa gagttcagtt gcttagagac
     5101 tatggaaatt gtttaagccc attcaatgcg tatcttacta atttaaatgt tgaaacacta
     5161 catcttagaa tggagagaca tagtgaaaat gcacttaaaa tagctagatt tttagaaaaa
     5221 catgaaaatg tagattggat taattaccca ggacttgaag ataacaagta ttatgagaat
     5281 gccaaaaagt atttatcaaa aggatgtagt ggtgttttat catttggagt aagaggtggg
     5341 ttagaaaatg ccaaaaaatt tgtggaaaaa ctacagatag catctttggt tacacatgtt
     5401 tcagatgtaa gaacttgtgt tatacatcca gcttcaacta ctcatagaca attaacagaa
     5461 gaacaattaa ttgcatctgg agtattgcct tcactaataa gattatctgt tggaatagaa
     5521 aatgcagagg atttaatagc tgatttaaat caagctttaa atttctaata ttctaagaga
     5581 aagagaagtg aaatgatatg gctttaatac taccaaaggg attacctgtt attaataaat
     5641 tattagatga aggaatagat gtaatttata aagaagattt taaaaaggaa ttaaagtgtg
     5701 aagaaaacat agacactaag atagctatac taaatctaat gcctataaag atagatacag
     5761 aattagattt actaagaagg atagataaaa caggattcaa tgtgtctgtg gaatttatta
     5821 aaatttcaac aagagaaagt aaaagaagtt gcaatgaata tgttaaagaa ttttataaaa
     5881 catttgatga agccaaagga gagtattttg atggctttat aataacagga gctccagttg
     5941 aacaaatgga gtttgaagaa gttgattatt ggaatgaact agaagaaatt atggattatt
     6001 ctaagagaaa aactaaatct acattatata tatgttgggc agctcaagca agtctttaca
     6061 aatactacaa tgtaaaaaaa cttccactaa gtcaaaaatg ttttggagta tttaagcata
     6121 aggtagataa aaatagtaaa atagtagatg gatttgaaaa tgaatttttt gctccacatt
     6181 caagacatac tactgtaaat attgaagctc tgaaagagaa taaagagttg agcattgtga
     6241 gtcattctaa agaagctggt ccctatataa ttacaaattc aagagatgta ttcgtaatgg
     6301 gtcatagcga gtatgacaaa tacactcttg acaaagaata taagagggat ataaataaag
     6361 gagataaaat atcgatacct caaaattatt atattaatga tgacccttca gaagagccaa
     6421 ctgtaaaatg gaaaaaacat tcagaattgt tgtttagaaa ttggataaaa aactatttga
     6481 tacaataaat tttaagagta tataaatgtt aactaaaaaa atatagaaat ttataggtat
     6541 aaagttaatc ttaatgatac tttatacctt atttttttaa aatttaaccc tataatagta
     6601 gttttatatt ttatagaaat aactttaatt gtagataaag gtagttttaa ttttatatat
     6661 ggtacgataa tggtagacta aacttttaga gtatcaataa atttgtattt taacttttaa
     6721 aagagattaa aaagggagaa atcagtgtaa aatatatata tatcgatttt ttattaaatt
     6781 ttattaaaaa tatcctgtat aaaattaaat atcaataaga taacaaaatt ccaattgaag
     6841 ttacaatatt gtaattgaca ataaatgctt aatactataa tatttaatac attaaacatt
     6901 atagtattaa gtatttattt acagtatgca cttcagccta aagttaaaat aaagtatttt
     6961 cgtagggagc gtttgtatgg atgagatgag ttttaagaaa gagctactag agttgactag
     7021 agatataaat atgaaattta caactttatt aagtgatttt tatcagccac ttggtattac
     7081 agcagtacaa gctttgattt tatctgagtt atgtgaacat ggagagaaaa aaataagcga
     7141 tttagggaaa aacttaaaca tgacaaatag taatgtatca gtaatctgtc aaaggcttga
     7201 aaaaaatggt tttttaaatc gcataagaga tatagaagac caacgaattg taaaggtaaa
     7261 agttacaaat aaatcattgg atatacaaga gcatatttca agtagtattt ttgatagtta
     7321 ttttgaaaat atgaccagtg aaaaattgca agatatggaa gatataatag aaggtttaga
     7381 gaaattaaat aaacttttaa cttacattga ttgcaaataa gataaaaata atttaaagga
     7441 gaataaaatt atggatgaac ataaacccca aaaaaggtct gcactctatt attatacagt
     7501 tataataata gtagtaatgt ttttaaatgc tttaatattt ccaagtttaa tggataaaaa
     7561 aatggttgag attgactatg ggaagtttct cacaatgttg gatacaggaa aggttaaatc
     7621 tgttgagata gaaaacaata gaatagcaat aaatccatct agtagcaagg ataagaatat
     7681 atacataact ggtagaatgg atgacccaga attggtagat agactaaaaa atgcaaaagt
     7741 agaatttact aaggttattc ctaaggaaaa ttcacctctt ttaagcattt tacttacatg
     7801 gataatacct ataggtattc ttatgctatt tggtaactta atgatgaagt ctatgcaaaa
     7861 gcgaatgggt ggtaatgcta tgcaatttgg aaaaagtaat gccaaaatgt atgtatctgc
     7921 tcaaagtgga aaaagattta atgacgtagc tggtcaagat gaggcaaaag aagctttaac
     7981 tgaaatagta gatttcttac acaatccaga aaagtataaa aagataggag cacaaatgcc
     8041 taaaggtgca cttttagtag gacctccagg tacaggaaaa acactccttg caaaagctgt
     8101 agcaggtgaa gcaaatgtac catttttctc aatatctggt tctgaatttg ttgagatgtt
     8161 tgtaggaatg ggagcttcta ggattcgtga tttatttaaa caagcaaaag aaaaagctcc
     8221 atgtatagtg tttatagatg agattgatac tattggtaaa aagcgtgata atggaagtgg
     8281 attaggtgga aatgatgaac gtgaacaaac tttaaatcag ctgctgagtg agatggatgg
     8341 ttttgatggt ggaattggag tagtaatact tgctgctaca aatagacctg acagtcttga
     8401 caaagcatta cttcgtccag gaagatttga tagacgtata ccagttgaac ttccagacct
     8461 tggaggacgt gaagctatat taaaggtaca tgctcaaaat gtaaatgtaa gctcagatat
     8521 agattataat gcaattgcaa ggtctacatc aggagcatca ggtgcagaac ttgcaaatat
     8581 tgtaaacgaa gcagcgcttt tagcagttaa gtctggcaga gatatagtag agcaacagga
     8641 ttttgatgaa tctgttgaga ctgtaattgc tggatatcaa cgtaaaggag ctgtaatttc
     8701 agacaaggaa aagaaaatta tagcatatca tgaaataggt catgcacttg ttgctgctat
     8761 gcaaaaacat tctgcacctg tccataagat tactataata cctagaactt caggagcatt
     8821 gggatatact atgcaagttg ctgaagatga gagtgtactt atgtctaaag aagaagcaat
     8881 tgataaaata actactttta caggtggtcg tgctgctgaa gaggttattt ttaacacttg
     8941 tacatcagga gcatcaaatg atatagaaca agctactaaa attgcaagag ctatggttac
     9001 tcgccttggt atgagtgaag agtttgatat ggtagctctt gaaactgtaa gcaatcaata
     9061 tttaggagga gatacttctc ttgcttgttc ttccgacact gctgctaaaa tagatgaaga
     9121 ggtatttaaa atagtaaagg cttgtcataa aaatgcaatt gaaatcttag aaaataatat
     9181 tgataaattg catgaacttg ctaattattt attggaaaaa gaaactatta caggtgatga
     9241 gtttatggaa atacttaact taaactaaaa tgattataca caagataaaa atgcctattg
     9301 ttaggcattt ttattttata actaaatttt ttaaattcat atttaaatct ttacataatc
     9361 ttaatcacta aaaatacatc ttaactacaa ttttatatcg aaattgatat tataatagta
     9421 atgaatggtg taaggaggtt ttttaatgga cagacctaat cctgatattc tattaaaaaa
     9481 aattaaaaat gaagaagaaa aactaagtag aggtcaatta aaaatattct ttggttatgc
     9541 agccggagta ggaaaaacct attctatgtt ggaatctgca caaaatctaa agaaagtggg
     9601 tgtagatgta gtagttggat atattgaacc acacactaga ccagaaacat tggctttact
     9661 tgatggcttg gaaattcttc ctgtaaagga gatagaatac aaatctataa aattaaaaga
     9721 atttgattta gatgctgctt tagcaagaaa accagaagtt atattagttg atgaatttgc
     9781 ccattctaat gttgctgggt taaggcatac aaaaagatgg caagatatag aagaattgct
     9841 gttagcaggc ataaatgtct acactactgt aaatgtacaa catttagaaa gtctaaatga
     9901 cattgttgag ataattacaa acgtatcagt taaagagact ataccagata agtttttgga
     9961 tacaaataca cagttagaac ttatagatgt tgaacctgat gtacttcttg aaaggttcaa
    10021 tgaaggtaag atatataaga aagaacaagc tactagagca aaaaataatt tttttataaa
    10081 agacaaccta gttgcactta gagaaatcgc acttagaaaa acagcagaga gagttaataa
    10141 agaagttcaa atgaccagac tatcaaaggg tgatgtaact gtaataccta catcagatac
    10201 acttcttgca tgtataagtc catctccatc ttcagcaaaa gttattagaa ctgcatccag
    10261 aatatcagac agtacatttg caaaatggat agctttatat gttgaaactc caaacacagc
    10321 aagacttcca tttgatgaac aaaaacaact acaaaataac ctaaaattag ctaagaaatt
    10381 aggtggagaa ataattgttt tacatggtga aaatataata gagcaaatac ttagaatttc
    10441 aaaactgaga aatgtaacaa aaattgtaat aggtagaaat cattcaagta ataaaagatt
    10501 tagtaaaaga tttaaaaaag atatggttga caaattaatt gatgaagttg attatataga
    10561 tatacatata attccatata aatttgcctc agatgttaaa tacaaaccta aaaaggataa
    10621 gtcatcaata aaatctaaat ttaaaattag caaagttgat tttataaaac ttatatttat
    10681 tactttgatg ataagtattt tagcatatgc agtacaaagt atgggattta taagagaaaa
    10741 tatattgtta atatatatgt tgggtgtagt tttagtttct atgtggacta aagggtatag
    10801 tacaggtata ataagttctg tatttaatat agttctttta aattattttt tcacagcacc
    10861 attatataca ttatctatag cagattcaaa ctatatagta acactggttg tattctcaat
    10921 agtaggtata ataactagca ctcttacatc taagatacaa catgaagcag aaactgctgc
    10981 taaaagagaa gaaaatacaa aaatgatata tcaaataatt aagggatttt taagattatc
    11041 aaataaagat gatatagtaa acaaaggcat agaacttttg tctcttagct taagtagaga
    11101 tgtaatttgt tatttaaatg tagataagta taataaatcg aaactttata aaaaaaatac
    11161 taataataag gataaaaatg atttggatag tgaagatgaa aaagctgttg caagttgggt
    11221 tttatctaat tcaacagtgg caggaaatga cacagataca cttcctggtt caaagggata
    11281 ctatatacca attgtaggga tgaatttaac tttaggagta gtaggaatat catgtattga
    11341 ttcaaaattg gatacagaag atataagctt aattgaaaca ataattgctc aaatggcaat
    11401 agcgcttgat agagaaatct tgtcagaagc taaagaaaat accaatctcg agattgaaag
    11461 ggaaagatta agaagtaatc ttttaagagc agtttcgcat gaccttagaa ctccgctagc
    11521 aggaatatct ggagcagtaa gcacaattat aaaaaataag ggtactatag gtcaagatat
    11581 aatagatgaa ttacttagtg gcgtatatga agatgctcaa tggttaataa gacttgttga
    11641 aaatttactt agtatgacca agatagatga agggaaattg gaagttaaaa aacattctga
    11701 actggttgag gagattatat cagaagcgtt acaaaaaata aaaaaaagaa ttgaaaatgc
    11761 acttatagat attaatatac cagaacagat attatttgtt cctatggatg caaaattaat
    11821 agaacaagtt ttaattaatt taatagataa ttctttgaaa tattcaaagg aagattgtaa
    11881 gatagagata aatgtatatg agaaagatga ttatgtttgg tttgaagtat ctgataatgg
    11941 tcctggtata tcaaaggaac ttaaaaagca tatctttgac agatttttta ctggagaaga
    12001 aggagctaaa gattccagaa agggtgttgg acttggatta tcaatttgta aatctataat
    12061 tcaagctcat aaaggtgaaa ttatggtaga aaacaataaa gataagggtt caacttttaa
    12121 gtttagtttg cctaaggaaa attaatagtt ttaaatttta aaattagttt tggaggaaga
    12181 aatgagtaaa aaacttatat tgttagtaga ggatgataag acaataagaa aatttatatc
    12241 tactgcactt ttaacacagg attatgatgt caaggaggct ataactggaa aagaaggtat
    12301 atctattgct gtctcatatt ctccagatgt tgttctttta gatttaggct tagaagatat
    12361 ggatggaata gaggtaataa aagctatcag acaattttca aatataccaa taattgtagt
    12421 ttcagctaga gaacaagata gagataaggt agaagtattt gatgcagggg cagatgatta
    12481 cttaacaaaa ccattctcca tagtagagtt attagcaaga gtaagagtcg catttagaca
    12541 ttcacaagta gaagctcaac aaaaagatga tgttaaatct acatttgaag tagataaatt
    12601 attgattgat tttgataaaa gaaaagttat tgttgatgat attgaagtcc atttgacacc
    12661 cattgaatat aatattcttt cattacttgc aaagcatcat ggaaaagtgc ttactcataa
    12721 ttttataata aaagaaatat ggggtagtgt tatagggaat gagactaagt ctttaagggt
    12781 ctttatggca acgttaagac gaaaaataga aaaacaacct gcaaacccaa gatatataat
    12841 tacagaagtt ggtgtaggat acagattaaa tgatgaatag ttgcaatat
//
LOCUS       DAEMAE010000102        12877 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEMAE010000102 DAEMAE010000000
VERSION     DAEMAE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.104"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            65..778
                     /locus_tag="KPH93_002231"
     CDS             65..778
                     /locus_tag="KPH93_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689041.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSEVIKVGDITIDIEGNRVYKDKNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1056..2594)
                     /locus_tag="KPH93_002232"
     CDS             complement(1056..2594)
                     /locus_tag="KPH93_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1689042.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGAGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINKDTTISLYLHTAFEKLYKIEKEMN
                     LLYQKSAISGNEQHLIKASDYQEQLIANNFYYIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSDYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            3476..3931
                     /locus_tag="KPH93_002233"
     CDS             3476..3931
                     /locus_tag="KPH93_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG1689043.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            3980..4564
                     /locus_tag="KPH93_002234"
     CDS             3980..4564
                     /locus_tag="KPH93_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG1689044.1"
                     /translation="MKILVFLAKGFETMEFSVFVDVVGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(4569..4892)
                     /locus_tag="KPH93_002235"
     CDS             complement(4569..4892)
                     /locus_tag="KPH93_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689045.1"
                     /translation="MKKCLDSYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            5044..5562
                     /locus_tag="KPH93_002236"
     CDS             5044..5562
                     /locus_tag="KPH93_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1689046.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(5738..6406)
                     /locus_tag="KPH93_002237"
     CDS             complement(5738..6406)
                     /locus_tag="KPH93_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689047.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKDSTIKN
                     IIEQHPEYNRKEKIKTEPASSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVDVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIELANGSEHFYGIGGKEQILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            6521..7936
                     /locus_tag="KPH93_002238"
     CDS             6521..7936
                     /locus_tag="KPH93_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1689048.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHQVDFKLNYTFRNEKDLHTN
                     KEKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIRVYPIQQCAIENILEYGHEIILGYAHLS
                     FDEIEKGVRILSKIIHMNQGT"
     gene            8159..8617
                     /locus_tag="KPH93_002239"
     CDS             8159..8617
                     /locus_tag="KPH93_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689049.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSKKCISYANMNYKQRRDEVNSYLNFNINCEVEVA"
     gene            8614..9198
                     /gene="clpP"
                     /locus_tag="KPH93_002240"
     CDS             8614..9198
                     /gene="clpP"
                     /locus_tag="KPH93_002240"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG1689050.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSTTAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTRGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKEQLDRILSENTGK
                     SIEDIRRDTDRDNFMTAIEAKEYGLIDYIMNRNE"
     gene            9510..10586
                     /locus_tag="KPH93_002241"
     CDS             9510..10586
                     /locus_tag="KPH93_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1689051.1"
                     /translation="MITISLCMIVKNEEDVIGRCLECVKDIVDEIIVVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYIMWLDADDIILEDDREKLLKVKETL
                     DTSIDIVMAKYNVSFDDNGKPTLSYYRERLFKRSSNHKWVDPIHEVIPLYGEIFYSEI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLGPRHQFYYSRELYYNARYEEAIEGFTKFL
                     DSSRGWLEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEVCCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            10653..12791
                     /locus_tag="KPH93_002242"
     CDS             10653..12791
                     /locus_tag="KPH93_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBG1689052.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNSGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGPTGNTGGTGTG
                     GPTGPTGPTGPTGPTGNTGGTGTGGPTGPTGPTGPTGNTGATGFGVTGPTGVTGATGA
                     TGADGVTGATGPTGATGADGATGPTGATGATGFGVTGPTGPTGPTGVGVTGATGLIGP
                     TGATGTPGATGPTGAVGATGIGITGPTGPTGATGADGATGVTGPTGATGATGADGVTG
                     PTGATGATGFGVTGPTGPTGATGIGITGATGLIGPTGATGATGATGPTGVTGATGAAG
                     LIGPTGATGATGADGATGATGATGATGPTGADGLVGPTGATGATGADGLVGPTGPTGA
                     TGVGITGATGATGATGPTGADGLVGPTGATGATGADGVAGPTGATGATGNTGADGATG
                     PTGATGPTGADGLVGPTGATGATGLAGATGATGPIGATGPTGADGATGATGATGPTGA
                     DGLVGPTGATGATGATGATGATGPTGATGPTGASAIIPFASGIPLSLTTIAGGLVGTP
                     GFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVG
                     STVTITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAET
                     RFLLVFTATASGLSLVNTVAGYASAGIAIN"
BASE COUNT         4764 a         1736 c         2275 g         4102 t
ORIGIN      
        1 aaaaaaacaa gatagtgata ctgcaagatt agaatataat aaatttttat ggagagattt
       61 caatatggat acatatttta ataaaaaaat attacttgtt gatgatgaaa aagatatagt
      121 agatttaatt gaagaagtac ttataaatga tggttttaaa aacattatta aagcttataa
      181 tggtttagat gctatttcgc tgtgtaagat agcatgtcca gatgttgtca ttcttgatat
      241 aatgttacca gatatagatg gtatagaagt gtgtaaaaaa attcgagagt tttcatactg
      301 ttctattctt tttctctcat caaaaaatga tgatattgac aaaatactag gtcttagtag
      361 tgggggagat gactatataa caaagccatt cagtccaaga gaaattgttt ttcgagttaa
      421 agcgcaatta cgtagacaac agtatcaaag tattgtacca tcagatagtg aagttattaa
      481 ggtaggagat attactattg atatagaagg aaaccgtgta tataaagata aaaatgagat
      541 tgagttaact ggaagggagt atcatctttt aagctacatg gctcaaaatg taaataaaat
      601 tataggaaaa gaaagactct atgaacaagt atggggagta tatagtagta tttgtgacaa
      661 tacaattatg gtgcatatac gacatataag agaaaaaatt gaagataacc cctcaaatcc
      721 taaaatactt ataacggtta aaggactagg atataaactt gtgaatagaa tcgattagaa
      781 tagattttaa tatcaattaa gatgtctcaa attgaatttt ttagttaatg aggcattctt
      841 ttatatgtga aatcaaatgt attttcgtta tctcaataat gaagaagagg tctatctcta
      901 ttttgagata gcctcattaa gtttatatcc tggcaatgta ctttatgttc taataaatat
      961 ttattttttt agtttatttg cagtgaaaca ttaactagaa cattaaaatt tgcattgaaa
     1021 taccaattaa ataatattgt atattgtgta taaacttata ctaattgttt ttctatatta
     1081 aatactttat caatccaacc tttataaaat ttttcttcat gggaaactag gattatactt
     1141 cctctaaact gtttcaaagc attttgtaaa gcatcctttg tctctgcatc aaagtgattg
     1201 gttggttcat ctagaataag aaaattacaa ggagataaca tcatacaaca taatttaacc
     1261 ttagattgtt caccaccact taaagtagat acacttcttg aaacatgctc ttccttcaca
     1321 ccacatctgg ctagatgacg tctaatctct tttgtattta attttggata tttatctgct
     1381 actatttgaa gtggggtttt atcaggattt tcccatttta aatcttgctc ataataacca
     1441 attttaactt gttctgaaaa ctggaaatct ccagatatac gaggaatatc tttgactaat
     1501 gtctttaata aggtagattt accgattcca ttaaaaccag ttataactat tttttgacct
     1561 ccatcaacag aaaaatttaa ttttggaagt aaagaatagt agtatccaac ttcaagattt
     1621 gttatagtaa gagcattttg agctgatatt tcaatttctg aaaattgaat atttggtttt
     1681 cctgtaaaac taggaggagc tattctttct attttatcca attgtttttg tcttcctctt
     1741 gcaatttttg aatttactcc agctttattt ttacgtataa atgtctcttc tttttctatt
     1801 ttcttttgtt gtgcttgata tcttctaata taatcttctc ttaaatgtga tttttgttta
     1861 aggaactctg aatattttcc atgatatttc ttaatcatgc caaattctat atcaagaata
     1921 cctgtagaaa ttttctctaa aaagtcaaaa tcgtgggaaa ctacaataaa tgcgccatta
     1981 aatgtattta aataatctga gagccattca acatgttctt tatctaaaaa gtttgttggt
     2041 tcgtctaata ataaaacatc atggttagaa agtagtaatt ttgctaaaat tacctttgcc
     2101 ctttgcccac cactaagctc tccaacaaca cgattcattc ctattgaatc taatccaaga
     2161 ccatttgcta ttttatttat ttcattatct atataataga aattatttgc tattagttgt
     2221 tcctgataat ctgatgcttt gattagatgt tgctcatttc cagatatagc acttttttga
     2281 tagagtaaat tcatttcctt ttctatttta taaagttttt caaatgcagt gtgtaaatag
     2341 agtgatattg ttgtatcttt attgatttct gcatattggt ctaaatgtcc tatattgata
     2401 ttaggttgcc acttgatttc tcctgaatct ggaacaactt ctcccaataa aattttaatt
     2461 aatgtacttt tgcctgcacc attttgacca actacaccta tatgttcacc tttgaataaa
     2521 tcaaagttag ctttctcata cagtgattta tccataaaac attgtgataa attagttact
     2581 tttaataaac tcataattca aactcctttt ttttattaaa aaatttatgg aaataaaaaa
     2641 agcagaggaa tggcattgta aaatgccgac ctctgctttt ggaaaaacaa aaggtcatag
     2701 acaagcattg ttgtctatga ccagaaaatc tatattatct atcggcaaat aggtaatttt
     2761 aaataagcat agccaaaaaa ctatactaaa cctatatttg ccgatgaaga tagaaagctt
     2821 taagctttca gattaacctc tacacaacgc ttcatcgtat acatttgtgt agagttaagt
     2881 tttctaacaa gtttaactaa ttctatataa atcataatta ttcacatcct aatcttagta
     2941 ttaattctag tttatctata tttaaattgt acttttgtac tttgtggatt atcttattta
     3001 tttaaaatat gttttattaa ttatagctta agttatgtta tcataatact tagttatagt
     3061 caataagaaa gttatattta ctatatatta tattaattta gcaaatctat cattacaaag
     3121 ttatttgaag attcactacc aataaagttt tatagtctgt aagaaaacta agattatgag
     3181 ttttataatt ttgatatagg gatactattt tcataaatat tataatatgt agaagtatat
     3241 gtctagtatt gttgaacttt caatgatata ttaaggttct aatatattat acaaaataaa
     3301 aacattaatt caacttaaga aattttaagt atcgtatgtg ttttgattaa atacacctaa
     3361 aattttaagt gtaaataaca tatttgcatt aaattttatt gaaatgatta aaattattta
     3421 cttaatgtaa attattagct aaaattgtac taaataatta aggaggtgag tagctttgga
     3481 tgagatagat ttaaaaataa ttaattctct aaaaaagaat agtagggctt caacctctga
     3541 aataagtaga caaattaatt tatcagtacc agcagtctca gagcgtataa ggaaaattga
     3601 gcagtcaaat ttaattcaaa agtacactat tcaaattaat cgggaaaaaa caaaatataa
     3661 gcttttggct tttatattta tcaatactga tttatctaaa agtatgaaag aatttaagaa
     3721 aactatattg gaatatgata ctgtattaga atgtcatcat atttcaggag agtatgatta
     3781 cttaataaaa gtattagcag aagatacaag taatcttgaa gagtttatat cagtgacttt
     3841 gaaggaaatt ggtgggattc agaaagctaa tacaaccatt gttttatcta caattaaaga
     3901 agaatttaat acaataaaag atatttttta aataattgac tagtaaagtg ttaattatat
     3961 tttaggagga aaatagaata tgaaaatttt agttttttta gcaaaaggtt ttgaaactat
     4021 ggaatttagt gtatttgtag atgttgtggg atgggctcgt aacgattatg ggcatgatat
     4081 tgatgtagtg acttgtggat ttaaaaaaca agttatgagc acatttaata tacaagtatt
     4141 agttgataag acgatagaag aagtatgtgt tgatgactat gatgctctag ctattcctgg
     4201 aggatttgaa gaatttggat tttatgatga agcatatgat tcatcttttt taaatctaat
     4261 aagggagttt aattcaaaag aaaaaataat cgcttctatt tgtgtggcag cgttgccagt
     4321 tggaaaaagt ggtgttttaa aaaatcgtaa agctactaca tatcatttga aaaatggtaa
     4381 aagacaaaga cagttaagcg agtttgatgt aaatgtagtt aatgaaccaa ttgttgtaga
     4441 taaaaatatt ataacctcat attgtccaga aacagctcca catgtagctt ttaagctttt
     4501 agaaatgttg acatctaaag aacaaatgga tgaagttaaa ttagcaatgg gatttaaact
     4561 gtaatggttt agagcttttc caagtaatca gaaccccagt cacacatgga atctaaaatt
     4621 ggaataatac tctttccaaa atcagatagt gagtattcta cttttggagg aacaacagga
     4681 taaaccactc ttataattaa accatctgat tctaattcac gtaattgttg agttaacatc
     4741 ttaggcgttg ctttaggaat taattttttt aattcattga acctcaatat agtgtcttta
     4801 agatgccaaa gtataagtgt cttatattta ccaccaatca atgcaagtgt tgcttctatt
     4861 gggcaactgt aactatctaa acattttttc atttttgggc gcctttattt attttaaagt
     4921 atctttttag gtactatatt acaaaaaagt gcattattgt aatatagtac catagtgata
     4981 aaattatagc ataggttaaa taaatcatca acttagccta ataaaacaat aggaggtaaa
     5041 taaatgaatt tcgttgaact tgcaaaaaaa agatattctt gccgtaatta ccaagataga
     5101 aaggttgaaa aagaaaaact tgagaaggtt ttagatgttg caagaatagc tccaactggt
     5161 ggtaatcgtc aacctcaaag attgattgtt attcaggaaa aagaaggtat aaataaactt
     5221 tctaaggctg ccaatatata tgatgcgcca ttagcaattc ttgtatgtgg agataaagac
     5281 aaagtttgga caagaccttt tgatgggaaa caacttacag atattgatac aagtatagtt
     5341 acagaccata tgatgttaca ggcaactgaa cttggattgg caagtgtttg ggtgtgctat
     5401 tttaatccag atataattag agaagagttt agtttgccag ataatctaga accaataaat
     5461 atattattga tgggatatga atctaagata ccagagagcc cagagagaca tgaaaaaact
     5521 cgtgttcctt taagtgaaat tgtttcttat gaaactcttt aattataaaa ttaagttatt
     5581 cataggataa atgtaataaa gtgtttatta taaacaaaat tcaaaataaa taatatctac
     5641 atataattaa ttttagtgaa gtatatctag tttaactaga atgtatactt cactaattta
     5701 tttattcaaa atatttatga ggattttaat atattaacta aaatatatca attgaattaa
     5761 cttttaaatc tggaaaagca cttgaaatat agtgttttat gtcatttaaa tggtcttgaa
     5821 tttgcttttc tgatatttta tcaggtccat atacaaaata cataatattt ttagtatctt
     5881 ttgttatagc tgttcgatta tcaggtccat taagaatact agataatata ccaatattat
     5941 ctgataaaaa taaatcatct tttgtcaaaa tctgttcttt accacctata ccataaaaat
     6001 gttcacttcc atttgctaat tctattttca aaggtaattc tattttatca aggtcatgac
     6061 ctgctgttag aagaagattt ttcaattctg ctaaaaacat tgactcaatt gttacatcta
     6121 catctgggat accttttgat tttaatagaa tagattctaa ttgaagcaat acagggtaag
     6181 ttttcttgaa ttttttataa tactttatat aacttgaagc aggctctgtt ttaatttttt
     6241 ctttacgatt gtattctgga tgttgttcta taatattttt aattgtagaa tctttaagaa
     6301 ttaaaaaatt ttctttatta ggttttgaat gtgtaatatt tataaccata actccaaatt
     6361 taatattggg ataaatttct ttaactttat gtgatatttc taacataata tagactcctt
     6421 tttatttttt attttatgat ataatcttag caagttaaaa gtggattttg tagttccatt
     6481 tttatagtaa ttttatagaa ccacttttgg aggtattaga atgtggatta cgattaatag
     6541 aaatagcaat atatcattat caagacaaat ttattgtgag ataaaagata tgattctaga
     6601 tggaagctta aagtttgagc aaaaattgcc atcatctagg actttatcaa aagaactatc
     6661 tgtatctcgt aatacagttt tagatgcata taaccaatta attgcagaag gatatttgga
     6721 aacaagaaat ggatatggaa caattgtagc taaaggaata tcagggcatc aagttgattt
     6781 taagttaaat tatactttta gaaatgaaaa agatttacat actaataaag aaaaaaatta
     6841 tatagatttt cgttcaggag ttccagaatt aaaactattt ccacgcaaag aactttcaaa
     6901 gctatataaa caaatactgt atgatttatc agattctgat ttcagatatg gaagtacagc
     6961 tggtgtatgg gttttaagag tggaaatatc taagtattta tttcgtacta gaggaattag
     7021 atgtaatcct aaaaacatta tgattgtttc tggttcaaca caaggtttat ctttaatttc
     7081 acaactttta tacaaaagta ataaaaaggt tgctgtagaa gaccctattc ataaaggctt
     7141 attaaaggtt atttcatcta ttggatattc ggttgttgga attaaagtag atgataaggg
     7201 aatgaatacc aacttaataa aatcaataaa ggggatttct tttgtttata ctacaccttc
     7261 tcatcaatat ccattaggag ggattttacc tattcaaaga agactagagc ttattaaatg
     7321 tgcaacagag aatgattact atataattga agatgattat gatagtgaat ttagatatga
     7381 gggtcaacca attagttctc tttatgaact taacccgaat aaagtaattt acataggttc
     7441 ttttagtaaa atattatctc cagctattcg tgttggattt atgttattac cagacaatat
     7501 tttagaaagc tataaaacct taaaaatgta ctctgatgtt catacagaaa caatattaca
     7561 acatgtatta gctgaattta ttggaaatgg aggtttagag aagtacatat ttagaatgaa
     7621 gaagttgtat aacagaaaac gtaagtacct cataaaagtt ttaaacacag aatttaaaaa
     7681 tgaatttaaa ataaaaggtg aagcagcagg acttcatata gttgctcatt tctataatgt
     7741 taaatttacg gaaagtttaa ttcataaaat ttttgaagca ggtataagag tttatcctat
     7801 acaacaatgt gcaattgaga acatattgga atatggccat gaaattatac taggttatgc
     7861 tcatcttagt tttgatgaaa ttgaaaaagg tgtaagaata ctcagcaaaa taatacatat
     7921 gaatcaaggt acatagataa aacatgtagc aatctacata taatagatgc aattaagttt
     7981 aaactttaaa agaaatatat attaatattc ttgtataaaa atacctgata ttgtgttatc
     8041 gatactttgt tgctatacac taaattaaga tagcaattta tattaagaaa aaactttttg
     8101 cataagttat aatacttaaa aggagaggat agagttgaaa acaaatacag atgttattat
     8161 ggagataatt gattatatag aagaaaatct tacagaaaat ctaaatttag ataaaatagc
     8221 tgatattgct aattattcaa aataccattt acacagaatg tttacaaatg ttgtaggatg
     8281 tactatacat caatacatac agaggaggag acttactgaa gcagctagac aacttatatt
     8341 tacagataaa tatattatag acatagctat tacagtagga tattctacac atcaatcttt
     8401 tacactagca tttagaaatt tatataaaga atctccacaa gtatatagaa ataaagatga
     8461 attttatcct attcaacaga gatttgtttt aaatctaaat catcaaattc aaagcaaaaa
     8521 atgtatttct tatgcaaata tgaattataa acaaagaaga gatgaagtaa attcttatct
     8581 taactttaat attaattgtg aggtggaagt agcatgagtt tagtaccaca tgtaattgaa
     8641 caaacaggtc aaggggaaag gtcttatgat atatattcaa aattattaaa agatagaatt
     8701 atatttatag gagaagaaat taatgatgct attgcaagtt tagtggtagc tcaattattg
     8761 tttttagaat cagaagaccc agattcagat ataattatat atataaatag ccctggtgga
     8821 tctactacag cagggtttgc tatatatgat actatgaatt atgtaagatg tgatgtatcg
     8881 actatttgta ttggaatggc tgcaagtatg agtgcttttt tacttgcagg aggaacacgt
     8941 ggaaaaaggt gtgctttacc caattcagag attatgattc atcaaccaat gggaggagct
     9001 aaaggtcaag caacagatgt aaaaatagca gtagataata tattgaaaat aaaagaacaa
     9061 ttagatagga ttctttctga aaatacaggt aaatcaatag aagatattag aagagataca
     9121 gatagagata attttatgac tgcaatagag gctaaagagt atgggctgat tgattatata
     9181 atgaatagaa atgaataaag tatcaaagta tcatataaaa atgtctgctt tacaaatata
     9241 tagagaatat taaatgagag tgtctcaaaa tgaacttttt agttcatggg acactctttt
     9301 ttgtatgaaa ttaaatatgt ttttattatt tcaacaatta ttatcaaatt tcaagtttaa
     9361 taaattctaa tataaaaaat acataaaaga taagactctt tagcaacttt ttgtcgttaa
     9421 aagtttttat aaatttaata acaaaataag ataaagtacc atatattact tatgtctgca
     9481 atatatagaa gacaaaggag ttgtacatta tgattacaat aagtttgtgc atgattgtta
     9541 agaatgagga agatgttata ggaagatgct tagaatgtgt aaaggatata gtagacgaaa
     9601 ttatagttgt agatactggt tctacagatt ccacaaaaga aatagtctca aaatatacag
     9661 acatggtgta cgatttcgaa tggatagatg atttctctgc tgcaagaaat ttttcatttt
     9721 ctaaagcttc taaagactat ataatgtggc ttgatgcaga tgacataatt ttagaagatg
     9781 atagagaaaa attattaaaa gttaaagaaa ctttggatac ttccattgat attgttatgg
     9841 caaaatacaa tgtatccttt gatgacaatg gtaaaccaac tctctcatat tatagagaac
     9901 gattgtttaa aagaagttct aatcataaat gggttgaccc tattcacgaa gtaatacctc
     9961 tttatggaga aatattttat tctgaaattg caatatctca taagaaactc catagacagg
    10021 accctctaag aaaccttagg atttttgaaa aaatgatttc agaaggaaaa gtattggggc
    10081 caagacacca attctattac tctagagagt tgtactataa tgctaggtat gaagaagcaa
    10141 tagaaggttt tactaagttt ttagactcat caagaggatg gcttgaggat tgtatcagtg
    10201 cttgcagaga tttagccaca tgttattact taattaatga tgaaaagagt gcattatatt
    10261 cattgtttag aagtttcgaa tttgatgagc caagagcaga agtttgttgt gacataggaa
    10321 agcatatgtt tgatagacaa aaatataaag aagccatttt ttggtataag gtagcattaa
    10381 caagagataa gaatgatact aatggaggct ttaagtcaaa tgattgttat ggatatatac
    10441 catctataca attaagtgtc tgttatgaca gattaggaga atcagataaa gctatttatt
    10501 atcatgaaaa aacaaaagaa atcaaaccta acgatagtgc tgttttgcac aatgaaaatt
    10561 attttagtaa atttaaaaat agttgatagg tcacaatcat actaaatttg acatattata
    10621 tatagagctt aaaagctcaa atatatcagg aggtgctttt aataatgagt agaaataaat
    10681 attttggacc atttgatgat aatgattaca acaatggcta tgataaatat gatgattgta
    10741 acagtggtcg tgatgattat aatagctgtg attgccatca ttgctgtcca ccatcatgtg
    10801 taggtccaac aggtccaatg ggtccaagag gtagaacagg cccaacaggc ccaacaggtc
    10861 caacaggtcc aggtgtaggg gcaacaggcc caacaggccc aacaggtcca acgggtccga
    10921 caggtccaac aggaaataca ggaggaactg gaacaggagg cccaacagga ccaacaggtc
    10981 caacgggtcc aacaggtcca acaggaaata caggaggaac tggaacagga ggcccaacag
    11041 gaccaacagg tccgacaggt ccaacaggaa atacaggagc aacaggattt ggagtaacag
    11101 gcccaacagg cgtaacagga gcaacaggag caacaggagc agatggagta acaggagcaa
    11161 caggcccaac aggagcaaca ggagcagatg gagcaacagg accaacagga gcaacaggag
    11221 caacaggatt tggagtgaca ggtccaacag gcccaacagg cccaacagga gtaggagtaa
    11281 caggagcaac aggattaata ggtccaacag gagcaacagg aacacctgga gcgacaggcc
    11341 caacaggggc agtaggagca acaggaatag gaataacagg tccaacaggt ccaacaggag
    11401 caacaggagc agacggagca acaggagtaa caggtccaac aggagcaaca ggggcaacag
    11461 gagcagatgg agtaacaggt ccaacagggg caacaggagc aacaggattt ggagtgacag
    11521 gcccaacagg tccaacagga gcaacaggaa taggaataac aggagcaaca gggttaatag
    11581 gaccaacagg agcaacgggg gcaacaggag caacaggccc aacaggagta acaggggcaa
    11641 caggagcagc aggactaata ggaccaacag gggcaacagg agcaaccgga gcagatggag
    11701 caaccggagc gacaggggca acaggagcaa caggtccaac aggagcagat ggattagtgg
    11761 gtccaacagg agcaacagga gcaacaggag cagatggatt agtaggccca acaggtccaa
    11821 caggggcaac aggagtagga ataacaggag caacaggagc aacaggagcg acaggtccaa
    11881 caggggcaga tggattagta ggtccaacag gagcgacggg agcaacagga gcagatggag
    11941 tagcaggtcc aacaggagca acaggggcaa caggaaatac aggagcagat ggagcaacag
    12001 gtccaacagg ggcaacaggt ccaacaggag cagacggatt agtaggtcca acaggagcaa
    12061 caggagcaac aggattagca ggagcaacag gagcaacagg cccaatagga gcaacaggtc
    12121 caacaggagc agatggagca acaggggcaa caggagcaac aggtccaaca ggggcagatg
    12181 gattagtagg tccaacagga gcaacgggag caacaggagc aacaggagcg accggagcaa
    12241 caggtccaac aggagcaaca ggcccaacag gtgctagtgc aataatacct tttgcatcag
    12301 gtataccact atcacttaca actatagctg gaggattagt aggtacacca ggttttgttg
    12361 gctttggtag ttcagctcca ggattaagta tagttggtgg agtaatagac cttacaaacg
    12421 cagcaggaac attgactaac tttgcatttt caatgccaag agctggaaca ataacatcta
    12481 tttcagcata cttcagtaca acagcagcac tttcacttgt tggctcaaca gttacaatta
    12541 cagcaacact ttatcaatct actgcaccaa ataactcatt tacagctgta ccaggagcga
    12601 cagttacact agctccacca cttacaggaa tattatcagt tggttcaatt tctagtggaa
    12661 ttgtaacagg attaaatata gcagcaacag cagaaactcg attcttacta gtatttactg
    12721 caacagcgtc aggtctttca ttagttaata ctgtagcagg atatgcaagt gcaggaattg
    12781 caataaatta gattaaataa ttaaaaaagg cttctagtat cctattgata ttagaagctt
    12841 ttttaagact gattgataaa catagtttta atattaa
//
LOCUS       DAEMAE010000103        12856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEMAE010000103 DAEMAE010000000
VERSION     DAEMAE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.105"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            186..896
                     /gene="rgaR"
                     /locus_tag="KPH93_002243"
     CDS             186..896
                     /gene="rgaR"
                     /locus_tag="KPH93_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG1689053.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1943..2974
                     /locus_tag="KPH93_002244"
     CDS             1943..2974
                     /locus_tag="KPH93_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG1689054.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWNSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3107..4405
                     /locus_tag="KPH93_002245"
     CDS             3107..4405
                     /locus_tag="KPH93_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG1689055.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILIECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVVRIIELI
                     KEKIEIMVREGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4519..4764)
                     /locus_tag="KPH93_002246"
     CDS             complement(4519..4764)
                     /locus_tag="KPH93_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG1689056.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4912..5778
                     /locus_tag="KPH93_002247"
     CDS             4912..5778
                     /locus_tag="KPH93_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG1689057.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5782..6318)
                     /locus_tag="KPH93_002248"
     CDS             complement(5782..6318)
                     /locus_tag="KPH93_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG1689058.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6509..6712)
                     /locus_tag="KPH93_002249"
     CDS             complement(6509..6712)
                     /locus_tag="KPH93_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG1689059.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7003..7767
                     /locus_tag="KPH93_002250"
     CDS             7003..7767
                     /locus_tag="KPH93_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1689060.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKIKN"
     gene            8156..9469
                     /gene="prdC"
                     /locus_tag="KPH93_002251"
     CDS             8156..9469
                     /gene="prdC"
                     /locus_tag="KPH93_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG1689061.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      9873..9958
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            10540..12657
                     /locus_tag="KPH93_002252"
     CDS             10540..12657
                     /locus_tag="KPH93_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG1689062.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTTFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQMPDGEKPSDGQIPDEKKPLDEQTPEEEKPLEEEIVIEEMSLKQD
                     IDKILDMTISQINKIVYNFWEDEENVKADEQSEINQVFTSEDSFISLWYDKKAKVKNS
                     CLLKEIDGSDRPYHDLRFDDITKTLTFDYVINGLVGASSKDNKYVIEGEDGEQTAFCY
                     NNHLRPPSSDGKSPYLPAEEFSGQENQNEDAVKSILYAGSEFDGFGYKQQFNLCEEEN
                     DIMTYSATQSAIWIILGQIDEQERLDQYQGSINLCDKLIERAKTEEERAEYQKKKEVA
                     INTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGV
                     VKLILPDGVTAYDGDGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKA
                     IQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDNVVNPPVP
                     PIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPTDDTVINPPVPPTDDAVLN
                     PPVPPTDDAVLNPPVPPTDDAVLNPPVPPVSDTVEKMPELSRDDTIVKSPKTGDETQL
                     MSYVFISVIAICGLVYQCKIKRN"
BASE COUNT         5181 a         1385 c         2172 g         4118 t
ORIGIN      
        1 ataatatatc tagaaaagta taggaattta tcgtaattta agtttaaata ttaaagagaa
       61 tttctataag tagtttaatc gacaatttaa atttataaag aaatttaaat ttgaaagcta
      121 gtgagtacag tttggaaatt gaaataaagt aaactaaatg atattttaga tttggaggtg
      181 atattatgtt cagggtagtt atatgtgatg acgaaaaaat acaaagaagc atactaaaag
      241 agtttacaga aaaacttttg tctgaaagat gtctaaatta tcaaatactg gaattttcct
      301 gtggagaaga tttaatatcc aaatacccag aaaaaattga tattatattt ttagatatcc
      361 aaatgaaaga tatcaatgga attgaggtgg ctaaaaaaat aagaaagttt gatgaaaaag
      421 tagaaattat atttacaaca gctttttcag agtatgcccc ccaaggctat gaggttcgtg
      481 cttatagata cttagtaaag cctatagagt atagtaactt tgcaatgggt gtaaatcttt
      541 gtattgataa ccttcaaaga aagaaggagc gttatatagt tttaaatagt aaaaaaggat
      601 ttaatagaat actgatagat tctattttat atgttgaaac tgtaaagaga gatttagttg
      661 tacatacaat aggtagagat tataaagcta atatgagtat gaaacaagca gaaaaattac
      721 tgtgtgaaaa tggctttttt agatgtcata caagttttat tgttaattta agaagaatag
      781 aagatcttag ggataatatg attactatta atgaacaatt tatacctata agtaagtata
      841 gacttaaaga tttaaaagta gcattaacaa gcttgttatg tgatataatg tattaatttt
      901 aaaaaggtaa ttgaaaatat cgtaaaaaag atttaatgtc atcacagggc tattgattaa
      961 aactgtctta atatataaaa tgttatttat attaagacag ttttattatg caaaatagca
     1021 gtttatagac ttttaatatt gtttaatatg tatatgtaga ggttccatta tagtagcttg
     1081 actatcaatt agtagcttga ttatcaatat actattattg atttttatat gcagtatatg
     1141 tatttttcga atatgattac ctttttaaaa taatgactat ttataaaact ctatagatat
     1201 ttgtctcatt tagctatgca tactttaatt aattatatta atatttaatg gaggacaata
     1261 taaatattaa agctatatct tatagtttat tgaacctata gtaatgaatt aataaatagt
     1321 ttaattctat gaaaagattt ccttttatgc aatttattta ttgaaatatg atataatata
     1381 tgaaggtgta agtattagtt gttaaaataa tatttatatt agtttttatg tatacttggt
     1441 gtaagttttt catatatcga ttaaaattga tgttgattta ttatttttag tattttttca
     1501 ccttttgatt catactttgt agaatggtgt attttctaaa actggttata agacaagttc
     1561 gttcaaatta gcatttttat agcatataaa taattgttcc ccctaaataa gatttagatg
     1621 tatagtatta aaatcaaaaa gtataaaata aaattgatgc ttaatcatag ataaatatta
     1681 ataaaatata caaggggatt gtctaataaa aaagataatc tcctttttta caaataattc
     1741 taaattgtga tataaatcta aaaattttat cttaacattt aaccatttaa tgataataat
     1801 tgatataata aatataaatt aaatatatgg tgtacttatt ttatgttttt agcaagaaat
     1861 atagcattta aaagataaat tgtgcaactt aaatcaaagt ttaagatgaa ctattaacat
     1921 agatatatta aaaacttttg taatggagaa tatatgtgat atgaataata aagttaaggt
     1981 tgcagtatta gatacaggaa tagacaaaga acatgattac ttaaaagata atttagttgg
     2041 aggaattgct tttgaatgta tgcatgatta tatctttata tcagataaat ttgatgatga
     2101 agatggtcat ggaactgctt gtgcatcaat aataaagaaa gagtatgaag atgtagagtt
     2161 atttgtaata aaaattttga gaaaagatgg tattacaaat ataaaagttc ttgaagaagc
     2221 attaagatac cttttagaca ctaacataag attaattaat ttaagtttat cagtcatagg
     2281 agtagaaagt gttaagggtt tatttgaagt ttgctatgaa ttgtttagaa aaggaaaaat
     2341 aatagtatgt tcactcgcta atgattttga cttaagttat ccagctatgt ttaacaatgt
     2401 tataggtgta aggggattta ctttagatgg agatgattca ttttggtaca acaaaaaata
     2461 tgatgttcaa tgtgttatgg atagcaattc atatataagt tgtgacataa ataattccta
     2521 taggttacca ccaaaatgta atagttattt atcagcaaag cttactggta aaatagctaa
     2581 gatattatca gaaaaaccag gcataacaat atctgattta aatgataagc ttgaatcatt
     2641 agcaactaga aatcattgga atagttgtga ttttgatgag tgtagtagga ttgtaaactt
     2701 taaattggat ttgtatgata aagaaaatgc tcttcttatg aaagttgctg atgttgttag
     2761 ggattgttta gatattgaag ataataatga aaaactcttt aagggtagcc tttttaataa
     2821 ggagataggc ttggtttatg ataattgttt taatttgttg aaaaagctag agcatagatt
     2881 tgacataaag tttaattata tggacatctc aaaatatgac ttagtttcaa tatatacact
     2941 gactgaatta gtggaaagat acaaaaactt ataaataaag atttgtagct aaatttaatg
     3001 tataaggtat taaattatat gtattctttg tcagtagatt aaaagtaaaa aatatactat
     3061 aattagataa cataagagaa aatgccaata caggaggaat tagataatga aatatgaaga
     3121 agcattagag tacatatcac aaactaataa attcggtata agattaggat tagaaaatat
     3181 cggaaagtta ttagaattat taggaaatcc acaagaaact ttaaatataa tacatgtagc
     3241 tggaactaat ggaaaaggtt ctgtgtgttc ttttgtttct aatatactaa tagagtgtgg
     3301 atataaagtt ggtttatata catcaccata tttagaaact tttactgagc gtataagagt
     3361 aaatggacaa aatataccac aagatgatgt tgttagaata attgagttaa taaaagaaaa
     3421 aatagagatt atggtaagag aaggctatgc atatccaaca gagtttgaag tggttactgc
     3481 aatggcattt tattactatt cagaacaaaa ggtagatttt gtggcattag aagttggttt
     3541 gggtggcaga tatgatgcta caaatataat aacaaaatca cttgtaagtg tgatagtatc
     3601 tataagtttg gaccatacag ggatacttgg agatacaata gaaaaaatag cgtatgaaaa
     3661 ggctggaata ataaaggaaa atggagtagt actagtatat gaccagactg atgaagcaaa
     3721 ggatgttata aagtcagttt gtaaagagaa aaaagctaaa tatatagaag ttgattttga
     3781 tgatataaac ataaaaaaat cagatataaa ttctcaaata tacgattgta ctgtaatgaa
     3841 agaaacttat agagaccttg aaataaaatt gataggcgaa catcaaataa acaattctat
     3901 cttggcaatt agtgtaataa aatatctaaa agatataaat aaattagcaa atataaatga
     3961 agaatctata agaaaaggac ttataaatac taaatggcca ggaagaatag aaaagataaa
     4021 agaaaatcca atttttataa tagatggtgc acataatgaa gatggagcaa aatctttggc
     4081 aaaagcactt gataagaatt ttaaaggtag aaaattaacc ttattaatag gtatgttaga
     4141 agataaagat attgatagtg ttttagagat attgctgcca cattttaata aggtaataac
     4201 aacaacacca agtaacccaa gggccataaa ttcagatata ttaagagaaa aagtattaaa
     4261 atatgtagat gatgtaacat caaagcatga gatagaggat gcagtaaatt atacattaga
     4321 aacatcaagt gaggatgata ttataataag tgcaggttca ttatatatga ttggtacagt
     4381 gagaacttta gtaaagaaat tataatttta taaaataaat atacaaataa aaatataaaa
     4441 aagagttgtc taaaaaatgg acaactcttt ttatataaag taagttttta ataattttac
     4501 tgatgtttat ataagtaatt atatagattg acgtatagct ttagataatt ggtctgggca
     4561 agaagtacct tttccaccac agtcaatacc gtttaattta tcagcaactt caagagcatt
     4621 ttggccaatt attaagcttc taagtcctga taaattacca ttacatcctc ctataaactc
     4681 agcatcaaca atcacattat tttcatcaac ttcaaaaatc atttctctac aacatacacc
     4741 acttggatta aatgttactt tcattaaaaa gacctccatt aaatattatt tcttttttta
     4801 acattaataa taataccaaa tatatgcgta ataatgcaac tataaagcat aatacaagct
     4861 aatgaaataa tatattattg tagtatatgt attgtagaga ggaggggtta tatgaaaatc
     4921 tatatttcta actacttatc aaagagtatt agcatagttg attattctac acttgaacta
     4981 gaaaaagaaa tagtattaga agataatata tatccacacc atttttgcat agaaaaagaa
     5041 aaaaatttaa tatacatacc tagttcaagt aatgggatac tctatgtctt agatttaagt
     5101 aatgataaaa taattgatac tgtctctata ggaggaagtc ttagtcaagt tgtacttagc
     5161 gataatgaat tgtttgtggc aaatgaagat tctaatagta tatatgtact agataaaaac
     5221 acattaaatc caataggaat tattggagtt gataatatgc cacatggatt tgactttgat
     5281 agagaaagca aaaagttata tgtaccgtgt ataaactcga tagtatgtat agatacaatt
     5341 aataaaaata tacaaaagaa aataaatatg gattttagag cttggcatat agagcttgat
     5401 aaacaaaaga aagaaatgta tatatcaaca cttgatggaa agcttataat aatagatgaa
     5461 gtaagcatgg atataaaaaa ggtattatat gaatttttat tacccgtaga aataagattt
     5521 aattatagtg gcaaaaaagt ttatgttgct gatttgggtt ataataatgt aagaatttta
     5581 gattatacaa cgagtagata cattggaaat attgaaataa atgggattcc ccaaggatta
     5641 gaaatatcta aagacgaaaa attattattt gtatcagata cacaagaaaa ttcagtaaaa
     5701 gtttatgaaa ctgcaaataa caaattaata aaagaaatca aggtaggaaa agagcctaca
     5761 actataattt gtttgtaggc tttaatgtat gtctcttata agtaaatcga taacttcggc
     5821 atacatataa gatgcattat ttttccagtt attgcaaata agttttgcct gtttctctga
     5881 tgaaacattt agatttaaat ctattaagtt agactgattt tctatgactc taaggttgac
     5941 aataaattca ttatcacttt gttttacaaa actagacttt acttgagtat ctgctagtag
     6001 attttctttg ttagaatcta tatacttgtc tattttttca gtaacactag taggtattcg
     6061 attaaagaag tattctagac tttctacgcc tctttgagta agagcataat attctctgtc
     6121 agagtcttta tatgtagtca aaaacttaga ttccataagc tgagataaaa gctgttgtaa
     6181 tgaaaaataa ttcatcatct cagtctctaa tactacttga gttatttgag aattagttaa
     6241 atccatttta attttttcta atatatataa aattaaaagt ttgtgatagg ctaattcttc
     6301 agacgagttt tcaaacataa atatcatcct ccgtcaaact atttctatat ataatatatt
     6361 aaactaattt gtacaaaatt aaaagaacaa tttgtattgt attattaatc tttagtaatt
     6421 catataaaaa cgattttata aataaatata gaaaaacagg gctgctaatt agcagccctg
     6481 ttgataccca ggtgtcaaaa tttactattt attttccagc catttgtctt tcagccattt
     6541 caactaattt cttagtcata tatccaccaa catatccatt ttctctagct gttaagttac
     6601 ctttgtctat gttttcatag ttagataaac ctatttcgtt agctatttct aatttcattt
     6661 ggtttaaagc agcttttgct tctggaacta ctgttctgtt tgaatttgac atagtaaatt
     6721 tcctcctcaa ataattagtt tatgatgtct aacaacactt ttatttatgt gatgttaata
     6781 ctaatttctc caaaataaaa ataatatata cagaaaaata aaggtaaaaa tgtaaaaaat
     6841 ataaaaaaat taaataaatg agtattttag agagtttaat taggtcaata taggttttat
     6901 ttttttagtt attgtcttat aaattaaata ttacagtact tttttgagct gaaaaaaaat
     6961 aaattttaaa tatagaagaa attatttcaa agggggaata gtatgaaaag agaagttaaa
     7021 aggactaaag ctttgatata tagtgtttta tttttttcat tattattagt gttaatattt
     7081 gtttttgttt atggaataaa atacatatgt tctaaatctg acaattcaaa aatgcctata
     7141 tataaagttg atacaaatga gaaaaaaata gctttgagtt ttgatgtagc atggggaact
     7201 gataatatgg atgatatttt aaaaatttta gataaacata atgtaaaagc taccttcttt
     7261 ttagttggta gttgggtgga tgataatgaa gaaattgtaa aagccataga tgaaaaagga
     7321 catgaaattg gaaatcattc aaatactcat gccaatttga aggaaatatc aaaagaagac
     7381 ataacaaaag agatagaaac tacatcagaa aaaatatata atataacagg gaaaaagact
     7441 aatttattta gacctccttt tggggacgta aacaataaag ctatggacat ttgtagtgat
     7501 ttaggttata aagtaattaa gtgggatgta gattctatag attggaagga gcttggacca
     7561 aatcatgtaa ttgaaaaagt aataaaagaa tctcaacctg gttcaatagt tttattccat
     7621 gctaatataa atgatgtaga taactatttg gatactataa taagtcgact aaaaaaagat
     7681 ggatacagtc tagtcaaaat atctgatttg ttgtataaag ataattatgt agttgattcc
     7741 aatggagtgc aaaaaattaa gaattaataa gtaataaatg tatattttaa gtaaagttgt
     7801 ggtaaattat atagtatatg ttagaaaagt gtcgataaaa agacggtgtc tattttttga
     7861 cactttttat acatgtatgc aatatcaata attatagatt aaattgatta aaaaagtgtc
     7921 taataattga cttttttttg atgggtttaa aaatttagaa gaattaatga atgctgtaag
     7981 acagtatttc caaagggtat aagtttgttt aaaagttggc atagaaattg ctttgttaat
     8041 aagcgatatg ttctatagaa ataaacaata tgttaagacc ttataataga aaaatagcat
     8101 taacattatt tgaacagctt ataaaaaaat taatatactt aagggggcgt actagttgaa
     8161 caaactatat tctattttat taaaacaaca cgttggtggt ccagataagc cagtagtaag
     8221 tgttggagat gtagtaaaaa aaggaactct aatcgcagaa ccaactggat taggggcaaa
     8281 tatttatgcc agtgttagtg gaaaaataag tgaaataaat gaccaagcga tagtaattga
     8341 agctgatgaa gtacaagaag atacttttga accattaaaa ggagaaggta tacttgactt
     8401 aattaaagaa gctggagtag taggaatggg tggagcagga ttccctactc atgtaaaatt
     8461 aaacatagat ttaaatggtg gaacaatatt agcaaatgca gctgaatgtg aacctttatt
     8521 agctcataat ataaaagaaa tagaagaaag accagaaata gtttatcaag gtataaaata
     8581 tgctatggaa gttactaatg ctggaaaagg tatgctagca ataaaaagca aacatccaaa
     8641 agcaatagaa gctttcaaaa aagtaataaa gccaggagac aacatagaag ttgcagaact
     8701 agttgatatg tacccaatgg gagaagagag agcaatagta agagacgttc taggaaaatt
     8761 acttgagcca actcaattac cttcagaggc taatgcagta gtaataaacg tagaaacact
     8821 aactagaata gtagaggctg ttgagcaaaa aagaccagtt atatctaaaa atataactgt
     8881 agttggtcaa ttaaacagtg gaaaagaatc tatagtattt gaagatgtgc caattggaac
     8941 aactgtcgga gaattaattg aaagagctgg tggaataaaa ggtgaatatg gtgaaataat
     9001 cctaggagga cctttcactg gtaaagctac tactttagat gctccaataa ctaaaactag
     9061 tggtggtata atagttacta tgccatttgt taatgaaaag agaaaaatgg gtcttttagt
     9121 ctgtgcatgt ggacctaatg aagagagaat gagagatata gcaactaaaa tgggagttac
     9181 tgatatagta tcagttcaaa aatgtaaaca agctcaagaa attaaaggtg cattaaaatg
     9241 tgaaaatcca ggacactgtc caggacaagc tcaaaaatgt atagaattta agaaagctgg
     9301 agcagaagtt atattaatag gaaactgtac tgactgtagt aacacagtaa tgggttctgc
     9361 accaaaatta aaattaggtg tatatcatat tactgaccat gtaatgagaa cagttaatca
     9421 cccattaata agaagaataa aaggtgacgt aagattaaaa ataaaataat agataaatag
     9481 aagttagaca tccttagaag taaaacttaa aagttacttc taaggatgtt ttttaatgta
     9541 ttttgagggc aatatatata ttgtaaaata aatggaaaaa atagacaaaa tatgctagat
     9601 attgtataat gataacaata gctattacaa ttaatattgg acaaaaataa tgatactcaa
     9661 atatgtggtt tgtattaata tgcatattat attaagtcaa ctatccaata gagtaaaagg
     9721 tttttgtgat gtttgaagat actctatagt atctttttta ttataaaaac taatttttgt
     9781 cttttattag ataagtatct aggatactaa tttatatttt attaagttgt taaaaagtgc
     9841 acttaaaaag ttaaaaaaga aaaattaaaa aacctattta tagaaactgt gaagtatatc
     9901 ttaaacttgg gcacttaaaa gatatatgga gttagtagtg caacctgcta taaatataaa
     9961 taggagtaac ttttaattgt caaaaatata taaaataagt acataaataa gtaattaata
    10021 gaagatagat ataaatatat ttttgaaatg ctccctgcat tcataacgaa tgtagagtgg
    10081 cagatatctg cccaaaactc ctttaattgc tggaagttcc taaagctaac taaaccacaa
    10141 cataatcatg aaataaggat aagtgtgata gagtgtgcat ttaataagca cttgaggtta
    10201 cgaaagtaga aaaaattagt tagatagcat aaggttaaat cctaagtgcc ttgaaaatgg
    10261 ataatcagca ggtaagcttc gaaaagaaga aacttcaacg actagagaga aatctcgtac
    10321 ctacaagtgt ggggaaatgg ggagtaccta atttatggtt aggtaatgat atagtctgtg
    10381 cttaatagaa atattaagaa gttcatagga gaactggtta ggagtaacga ccctaattga
    10441 acacactaaa gatatattat tgaataagat tcatataata tttaataatt ttaattatat
    10501 taagtgaata caatatatgt ttttagtatc aggagaattt tgaaaacaaa aattaaaaaa
    10561 tcaagtataa tcagtttagt aattgctttt agtatggtat ttactacatt tacaccaata
    10621 gtatcttatg ctgacgaagt gactagcaat gatacaatac ttaatggaga agaacaatca
    10681 aatggtcaga caccagatgt agagaaacca tcagatggtc aggtaccaga tggggaaaaa
    10741 ccatcagacg gtcagatacc agatggggaa aaaccatcag acggtcagat accagatggg
    10801 gagaaaccat cagacggtca gataccagat ggggaaaaac catcagacgg tcagatacca
    10861 gatggggaga aaccatcaga tggtcagatg ccagatgggg agaaaccatc agatggtcag
    10921 ataccagatg agaagaaacc actagatgag caaactccag aagaagaaaa accattggaa
    10981 gaagaaatag ttatagagga aatgagttta aaacaagata ttgataaaat tcttgatatg
    11041 actatatctc aaatcaataa aatagtatat aacttttggg aagatgaaga aaatgtaaag
    11101 gcagatgaac aatcagaaat taaccaagtc tttacttcag aagactcgtt tattagctta
    11161 tggtatgaca aaaaagcaaa ggtaaaaaat tcttgtttac taaaagaaat agatggttca
    11221 gatagacctt atcatgattt aagatttgat gatattacaa agactttaac ttttgactat
    11281 gtaataaatg gtcttgtagg agcaagctca aaagacaaca aatatgtaat agaaggtgaa
    11341 gatggagagc aaactgcatt ttgttacaat aatcatttaa gaccaccaag tagtgatgga
    11401 aagagtccat atttaccagc agaagaattt agtggtcaag aaaatcaaaa tgaagatgca
    11461 gttaaatcta tactatatgc aggttctgaa tttgatggat ttggatataa gcaacaattt
    11521 aatttatgcg aagaagaaaa tgacataatg acttactcag ctactcaaag tgcaatttgg
    11581 attattcttg gacaaataga tgaacaagaa agacttgacc agtatcaagg ttctataaac
    11641 ctttgtgata aacttataga aagagcaaaa actgaagagg aaagagctga gtatcagaag
    11701 aagaaagaag tagcgataaa tacaaaagct tatttagaag cattgttaaa agcaggtcgt
    11761 gaagagttaa agccgaatga tacaggaaaa cctagtttga gtaatggttt aactaagatt
    11821 aactttgaaa aaaatgaaga tggtacttat gaaacagaag ctgtagcctt agtaggttat
    11881 agtggtgtag taaagttaat actaccagat ggagtaactg cttatgatgg agatggtaat
    11941 ataataggta caggtgaagt agaaatttca acacaacaga aatttaaact taagagtgtt
    12001 ggaaaaccag atgccaaagc taatatttct gctgtatctt atgattatat ttttccaaaa
    12061 gctattcaat attataaggc agtgcttgat ttaggacaaa aagaccattc atcttcatta
    12121 ccatctagta agcaaaattt attaagttat acaatagaga aaaagaatgg tcaagaagtt
    12181 aactttaata taggtctacc aacagatgat gataatgttg taaatccacc agtgccacca
    12241 atagatgatg atgttgtaaa tccaccagtg ccaccaacag atgatacaaa tggacataag
    12301 ccaaaaccat caccaccaat agatgataca gtaataaatc caccagtacc accaacggat
    12361 gatacagtaa taaatccacc agtaccacca acggatgatg cagtgctaaa tccaccagta
    12421 ccaccaacgg atgatgcagt gctaaatcca ccagtgccac caacggatga tgcagtgcta
    12481 aatccaccag taccaccagt aagtgataca gtggaaaaaa tgccagaact atcaagagat
    12541 gatacaatag taaaatcacc aaaaacaggt gatgaaactc agctaatgtc atatgtattt
    12601 attagtgtca ttgcaatttg tggattagtg tatcaatgta aaataaaaag aaattaatct
    12661 agattattat taagtttaaa ataaaaagtc tcaaattgat ttataagtca ttttgggact
    12721 tttttatgta aatataatat gcaaatatag tttgtttaaa aatatctatt ttaattatat
    12781 tcttaaattt atgattagat acaggtattc attaattgtt tgatgaatag aaaaatgttt
    12841 tgaaggtgaa aatagt
//
LOCUS       DAEMAE010000104        12734 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEMAE010000104 DAEMAE010000000
VERSION     DAEMAE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.106"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            305..1198
                     /locus_tag="KPH93_002253"
     CDS             305..1198
                     /locus_tag="KPH93_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG1689063.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELKNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1198..2592
                     /gene="gltA"
                     /locus_tag="KPH93_002254"
     CDS             1198..2592
                     /gene="gltA"
                     /locus_tag="KPH93_002254"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG1689064.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            2958..5177
                     /locus_tag="KPH93_002255"
     CDS             2958..5177
                     /locus_tag="KPH93_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1689065.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNVSSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIIKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKILDL
                     EGNIIGTTKSEILKNINKKCQGNFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYSEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLITTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LGDVNEIEGAEALIRWNSPEFGFISPIDFIPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5324..6070
                     /locus_tag="KPH93_002256"
     CDS             5324..6070
                     /locus_tag="KPH93_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689066.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVILDGYDVSKLEGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLDGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSRSTDDVLKLFTRINKEQNVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6070..7950
                     /locus_tag="KPH93_002257"
     CDS             6070..7950
                     /locus_tag="KPH93_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689067.1"
                     /translation="MDFRKFAYNNVVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGIQSGSTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAILLTIVS
                     FMILFLITGPISLKLLSKKSILELLIGTKKPKKEIKSSKLFGIIGAVCLILGYAMIIF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNNEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGESSKNNFPMVYSYFSAHNNEKEYEE
                     LQQIRDAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSISQEHINSLKQLKNYNIKNLNIEVKKSTNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFNLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8069..9460
                     /locus_tag="KPH93_002258"
     CDS             8069..9460
                     /locus_tag="KPH93_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1689068.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VIMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9567..10607
                     /locus_tag="KPH93_002259"
     CDS             9567..10607
                     /locus_tag="KPH93_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1689069.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11093..11746
                     /locus_tag="KPH93_002260"
     CDS             11093..11746
                     /locus_tag="KPH93_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1689070.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKNFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDDMKF"
     gene            11822..12031
                     /locus_tag="KPH93_002261"
     CDS             11822..12031
                     /locus_tag="KPH93_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689071.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12171..12734)
                     /locus_tag="KPH93_002262"
     CDS             complement(12171..12734)
                     /locus_tag="KPH93_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG1689072.1"
                     /translation="MNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSAN
                     KRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRIL
                     HGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIALL
                     SLTSMADQLKNISQKLVDRYFPDITKK"
BASE COUNT         5031 a         1247 c         2192 g         4264 t
ORIGIN      
        1 gtatttataa aatttttgtt acaaatttgg cataatatag tatactttaa gtgtgtatta
       61 ggttaaatct ataacaaatt agacatatga tatagactta gtgtttattg gcatttataa
      121 gaaatttgtt aatatcaact atttgtagtt tgggcagtta taattactat aggggggatg
      181 tccaatacaa aacaatcaac caagaactaa tattatatta taaagagtta tagttttgtg
      241 tgtttatgtt tttaattaaa tttaaagtgg gaaaaatata tttgttagaa tggagaatag
      301 cgagatgagt aataaaatag ttagtaagag acaattaaca gatagtattt atttaatgga
      361 aatagaggca cctagagttg caaaatcttc tcaaccaggt caatttataa taattaaaaa
      421 tgatgaaaag ggagagagga ttcctcttac aatagctgac tatgatagag aaaaaggaac
      481 agtaactatt gtattccaaa cagttggtgc ttcaactaaa aagttagcaa tgtttgaaga
      541 aaacgatttt gtaatggact ttgtaggacc attaggacaa gcaagtgaat ttatacatga
      601 agatatagaa gaattaaaaa ataaaaagat attatttgta gctggtggag ttggttctgc
      661 tccagtttat ccacaagtta aatggtttaa agagcatgga ttagatgtag atgtaataat
      721 tggtgcaaga actaaagaat taattatatt agaagatgat atgaaaaaag tagctaaaaa
      781 tgtatatgta tcaacagatg atggtacata tggatttaat ggaagagtta cagacctatt
      841 aaaagattta gttgataatc aaggtaaaaa atacgaccaa gcaatagtaa ttggaccaat
      901 gataatgatg aaatttatgt gccaattaac taaagaacta aatataccta ctatagtaag
      961 tttaaataca ataatgatag atggaacagg tatgtgtggt ggttgtagag ttagtgttgg
     1021 aaatgaaact aagtttgcat gtgtagatgg tcctgaattt gatggtcatt tagttgattt
     1081 tgatcaagca atgagaagac agtctatgta caagactcaa gagggaagag ctatgctaaa
     1141 acttgaagaa ggcgatagtc atcatcatag taattgtgga tgtggaggta acaaataatg
     1201 gatgctaaaa aagtaaaagt accagttaga gaacaagaac ctgctgtgag agcaacaaat
     1261 tttgatgaag tttgcttagg gtataataaa gaagaagcta tggcagaagc taatagatgt
     1321 ttagcttgta aaaaaccaaa atgtgtaggt ggatgtccag taggaatcga cataccaggg
     1381 tttataacaa aaattaaaga agatgatata gaaggtgctg ctaaagtaat agctaagagc
     1441 agttcattac cagctgtttg tggtagggta tgcccacaag aaagtcaatg tgaaggtgta
     1501 tgtatacttg gaataaaatc agatgctgta tcaataggta agttagaaag atttgtagca
     1561 gattggtcaa aagaaaatga cataaattta tctgatacag agcctaagaa aaatcaaaaa
     1621 gtagcagtaa taggaagtgg tccagcaggt ctagcttgtg ctggtgattt agctaaaaaa
     1681 ggctatgatg taacaatctt tgaagcaatg catgagccag gtggagtttt aacttatgga
     1741 atacctgagt ttagacttcc aaaacaagct gttgttcaac ctgaaataga taatataaga
     1801 aaattaggtg taaaaataga aactaatgta attgttggta aaacaattac agtagatgaa
     1861 cttatagaag atgaaggatt tgaagctata ttcataggtt caggagctgg tcttccaatg
     1921 tttatgaata taccagggga aaatgcaaat ggagtatttt ctgcaaatga gtttttaact
     1981 agagtaaact taatgaaggc ttatagagat gactatgata cacctattag ttctggtaaa
     2041 aaagttgctg tagtaggtgg tggaaatgtt gctatggacg ctgctagaac tgcactaaga
     2101 ttaggttcag aatcgtatat agtttataga agaagtgaaa aagagcttcc agcaagagct
     2161 gaagaagttc atcatgcaaa agaagaaggt attatattta atacattaac aaatcctaag
     2221 gaaattttag tagatgaaaa tggatatgtt aaaggtatgg tttgtataag aatggaatta
     2281 ggagagccag atgattctgg aagaagaaga cctatagaaa tagaaggttc tgaatttgtg
     2341 cttgatgtag acacagttat aatgtcactt ggaacaagcc caaatccact aatatcttct
     2401 acaacaaaga gtcttgatat aaataagaag agatgcttaa taacagatga aaatggtcag
     2461 acttctaaag agggggtatt tgcaggtgga gatgctgtta ctggagcagc aacagtaata
     2521 tctgcaatgg gtgctggtaa aacagcagca gcatcaatag atgaatattt aaaagcaaaa
     2581 gttaatgctt aatttgtaaa ttaaagagag ggtgtctcaa aatgaacttt ttagttcatg
     2641 aggcactctt tttgtacaaa atcaaatatg ttttaatcat ttcaactatg aaaatttcat
     2701 tcctgtagaa agtctaactt ttaagtctta gtacaatctc tattttgata tagccacttt
     2761 tctagatggt ttaataattt atcagttatt attgtaatat ttgatactaa ttaacataac
     2821 tttgtctaac tatgataaaa tagttataca ataaaggtct tgtaaggagt gaataatgtg
     2881 tggctaataa aatcagattt gtcaaatcta acattaaata atagaaaaat aaattgtgta
     2941 attaatggta ttttaatatg aaaaaaagag aaaaagtatt tgatgggcta gtttttaagc
     3001 atatattaat cttattaata ctattagttt taattgattt aacaagcacc atcatatatg
     3061 caagtaactc tatggaaatt acttctagaa atatgattga ggcatcaaaa agagaacttg
     3121 aaaactattt agaagtaaac atatctttat taaaagctct tagtcaagat gatagatttt
     3181 ctaatggtga aacatcacta attgaaaagg gaaaattatt aaggccatat caaaaggaat
     3241 ataatctttt tatgattggt ataacagata caaaaggaaa tgtttcaagt acatataggg
     3301 aaaatgttaa ctcaataaaa gataagccta gttttgagaa agcaattaaa acaaagcaag
     3361 tggtagtttc agatattgaa gtcagtaatg taacaggtga taaagtattt atcatatatg
     3421 taccaattat aaaaaataat gagatgattg ggaccatttt tgcttctttt tattttcaag
     3481 atgtaaataa tttaataagt agaagcaatt ttgatgattc tattaaattt ctaatgatag
     3541 ataaagatta tactattatt tctcatccaa acaaaaaata tgtaaatgat aaaagtaaga
     3601 ttcttgattt agaagggaat attataggaa ccactaaaag tgaaatttta aaaaatataa
     3661 ataaaaaatg tcaaggtaat tttttatctt gggataattg gagactatat aatgtaaaat
     3721 acacgaatat aaagtggacc aattggactt tagtatcaaa atgtaatata tttaagaact
     3781 tcaaaaatct aatagttaat tttatgataa agttgtattt ttatatagta atatttatga
     3841 tattgtggaa attaagtaat gctaaattaa tagaacaact taaaaaattg gcttattatg
     3901 attctttaag tggaataaaa aataaagaaa agtttagaaa agattctatg tatattttaa
     3961 agaattatta tcaagataat ttttacttag tacaattgga tgtcaacaaa tttaaatata
     4021 taaatgagat gtttggatat tctgaaggga ataagatatt aattcatata tcacaggttt
     4081 taaacaataa tacaaataaa tatgaaattt gcgctaggat ggataatgac cattttatat
     4141 tgttgattgc ttgtaacact gaagatgaac ttttaaatag attatctaaa ataaataaag
     4201 aaatttgtaa cttaattact actaactcat ctaagtataa aattgttatg tcaagtggaa
     4261 tatataagat tactaaaaag gatgatatta aaaagattga cttgttaatt gatagagcaa
     4321 atattgcagc aaaaagtaaa aaggaaaaat atgaacattc atattcgttt tttaatgaag
     4381 atacaagaaa tcgattgtat aaggaaaaac gattggaaga taatatgaat aaagctttag
     4441 aaaaaggtga atttatagtg tattatcaac ctaagtatag tttaggtgat gtaaatgaaa
     4501 ttgaaggagc agaagccttg ataagatgga atagtccaga atttgggttt atttcaccta
     4561 tagattttat acctttattt gaaaagaatg gttttattgt aaatattgat atgttcgtgt
     4621 ttgaagaagt atgcaaaacg ttaaacaagt ggataaataa agggtatact cctgtaccaa
     4681 tctctgtaaa tatgtctaga gttcatttat atagggacaa ttttatagaa aatataactg
     4741 atttgataag taaatacaat atatctcctg agtttattga acttgaacta acagaaagtg
     4801 ttgtatttga taatttaaat atattgattg atataatgaa aaagattaaa gagataggat
     4861 tccttatatc aatggatgat tttggttcgg gatattcatc tttaaatctg ttaaaagacc
     4921 tttcttttga tattttaaaa ttagatagag gatttctaat tgaaacaaca gatacaaaac
     4981 gtggaaaaat tattatttct aagattgtgg aaatggcaaa agcgattgat attaaagtaa
     5041 tctgtgaggg cgtagaaacc tatgaacaag tagagttctt gaaagagata ggttgtgata
     5101 aggttcaagg atacttattt gctaaaccta tggtattaga tgaatttgaa aagcacttaa
     5161 actttaaatt tgattaatca ttaataattc ttagactatg agatacccta acaacttact
     5221 tacaaatatg taagttaatt gttagggtat ttttatggtt aaagtagtat attcacgata
     5281 ttatggtatt gtagtaaaaa taaaaaatta tggagaatat aaaatgaaca tattgagtat
     5341 tagaaatata tcaaaaacat attgtggaaa tattccattt aaagcattag acaaggttag
     5401 tctaaatata gaaaagggag aatttgtgtc tgttatggga ccatctggaa gtggtaaatc
     5461 tactctttta aatataatat ctacagttga tagacagagt gaaggtgagg ttatattaga
     5521 tggatatgat gtaagtaaat tagaaggaga aaaacttgct gaatttagaa ggaaacaact
     5581 tggttttgta tttcaggatt ttaatttaat tgacacttta actgttggcg aaaatattat
     5641 gttgccactt acactagatg gggcaagtat caaagatatg aatatacaaa ctaaaagtat
     5701 atctaaattt ttaggaatag ataaaatttt agatagaaaa acctatgaaa tttcaggagg
     5761 gcaagctcaa aggtgtgcta ttgctagagc tattataaat aaacctgcca tacttcttgc
     5821 agatgagcca actggaaatc ttgattcaag atctactgat gatgttttga aattatttac
     5881 taggataaat aaagaacaaa atgtaacaac acttatggta actcatgaag catatagtgc
     5941 aagttattca gatagagtaa tttttataaa agatggttgt atatatacag aaataaaaaa
     6001 atctgaaagc agtaattctt tctattctga tatattagct gtattgtctc aaataggagg
     6061 tgtaagatag tggattttag aaagtttgct tataataatg tagttagaaa ttttagagca
     6121 tatttagcat attttttaag ttctgttttt tctataatga tattttttgt ttttgcagtg
     6181 tctatgttcc atccaattat aacaaaatct ggaattcaaa gtggttctac tgcatttatg
     6241 gctcttatga cttcagaatt aataatatta ggttttagtc tactttttat attgtactct
     6301 ttaggaaatt tcttaaaata tagattgaaa gattttggag tattgatgat aattggaatg
     6361 agtaacagac aactgaaaaa attgatatta attgaaaatt tgataatagg gtttttagca
     6421 gtgatgttag gaatattact tggtttatca atttccaaat tgtttttatt gtatttaagt
     6481 aaactgtttt atatgaattt aactgaattt tattttccag taaaagcaat actattgact
     6541 attgtatcat ttatgatttt gtttttaata acaggaccaa ttagcttaaa attattgagt
     6601 aaaaagagca ttttagagct tttaataggt acaaaaaagc ctaaaaaaga gataaaatct
     6661 tcaaagctat ttggaataat tggtgcagtg tgtcttatat taggatatgc tatgataata
     6721 tttggcaata agttaaaaat aaatggaggt atgtatacaa cagcactatt aattactttg
     6781 gggatatttt tatttttttc tcaatttacg attttgatat taaagtattt aaaaaataac
     6841 gaaaaatttt ataagaacaa agtaaatatg ttacttataa gtaatttgat atataaatta
     6901 aaggataata cacgattgtt atttttaata acaatacttc tatcggctac tttagtagca
     6961 tttacaacaa cttcaacact tgtaagttct caaggagaat cttctaaaaa taatttccca
     7021 atggtttact catatttttc agcgcataat aatgaaaaag aatatgaaga actacaacaa
     7081 atcagagatg caatcaaagg atatgattat aaggagttaa gttttcctgt acttaaatac
     7141 aatagggaag tcttactaag tgtaaagaat tataacaaat tatcaaagga attggggtta
     7201 gataaaatta gttcaaagtt agaaaaatat gaaggaatta taattcccag tagcatttcg
     7261 caagaacata taaattcttt aaaacaatta aaaaattata atataaaaaa tttaaatata
     7321 gaagtaaaaa aatcaacaaa taaaataatt tttcctacag gactgtttag taaagtcgta
     7381 gttataagtg atgacttata caatgaagta aaaggtgaat tttcaaaaat taattttcat
     7441 ggatttgatt ataaaaactg gcaaagcagt gcaaagataa ctgaaaattt aaaagaaaaa
     7501 cattttaatt tggatagaga atataacagg tcatattttt taacattacc agatatgtat
     7561 ttagtagagt tacaacaaag taaaattctt caatttatgg gagtttttat aggagtgata
     7621 ttatttatag cagtttttag ttttttatac ttcagattat atacagatga gatagaagat
     7681 aatgtaaagt acaaaaaact atcaaaaata ggtctatctt ttaaaaatat gaataagatt
     7741 gttagtatag agataggtac attgtttttt ataccgtatt ttatagcaat tataaatagc
     7801 ataatttctt tactgttttt aaatcaagta ttaaataata gtttcaactt agaaaatttg
     7861 ggtatattac tactgatatc atctatatat tcactttatt tttacctatt aaaaaaaaat
     7921 tatactagaa aagtttggtt ttttgattaa agtggtatat aatagatata taaattaata
     7981 tttaaaaagt agttaatatt aggggagggc atttgcacct cccttgtaat atgtggaaaa
     8041 ttgaatatta gaagaaggag gagacttgat ggaaaatcaa caattattag gaacagaacg
     8101 aattagtaaa ttactactaa aatattctat accagctata ataggtatgt tggtaaatag
     8161 tttatataat gtagtagata gaatttttat tgggaatata ccaggggttg gaccacttgc
     8221 aataacaggt cttggtgtaa caatgccaat aatgacaata atacttgcat ttgggatgtt
     8281 gataggtata ggaactacta ctaccatatc aataaaactt gggcaaggta aagtagaaga
     8341 ggcaagaaaa cttataggaa atgcaatgac gttatctgta ataacaggaa ttatcataat
     8401 gatacttgga atattatttg caaataaaat acttacattg tttggagcaa gtgaaaatac
     8461 tttaatatat gcaaaatcat atataaatat tatattatta ggtacagtag taaatcttct
     8521 ttccttttct ttaaaccact caataagagc tgatggtagc cctaaaattt cagcaggaat
     8581 aatgatagta ggatgtttaa ctaatatagt tttagactgg atattaatat ttggattcaa
     8641 tttgggaata caaggagctg caattgctac agtaacttct caagctctta cagctatttt
     8701 aactataggg tattatataa gtggaaaatc aaacttacga ttcagtaaat ctaatctaaa
     8761 attagataaa aagctaataa aagcagtatt tgcaataggt atgtcgccat ttgcgatgca
     8821 acttgcagca agtttagtcc aagtaatatc aaatattgca cttaaaactc atggtggaga
     8881 tttagctata ggggctatgg ctactatttc ttctattgca atggtatttt tgatgcctat
     8941 ctttggaata aatcaaggag cgcaacctat tataggtttc aattatggag ctgaaaaata
     9001 tgatagggtt aaaaaagcat atttaggttc attagtagta gctactataa ttttatgtat
     9061 gggtatggta gtaattatgc ttttcccaga agctattata ggtatattta ataaagaccc
     9121 tgaacttatg aatatatctg ttaatggatt aagaatatac ttacttatgt tgccaattgt
     9181 aggcttatca gttacaggaa caaactttat acaatctata ggaaaagcaa aaatggctat
     9241 gttattgagc cttttaagac aagtaatttt attgatacca gcagtattaa ttcttcctac
     9301 attcttaggt ttacagggtg tatggacagc tcaaccagtg tctgatttta tagctacagt
     9361 aattacaggt atagttgtat ttagagaatt aaaaagatat actcctaaaa ctgaaaaatt
     9421 aaatgaaaat gaaagactaa atgagataac tactgagtaa aaatataaat tcagataatt
     9481 gagcaagaaa ttgcaaaaaa tacaattata tgataaaata aaaccgctgt caaggataag
     9541 agaaattcta attatggagt gattttatgg aatttacaaa gaaaagcaaa ttaaaaaata
     9601 tgtatgtaaa catgaatgta gctagaatgt tgtctaagat aaatgagtat aagggtagac
     9661 aactgcttta caaaaaacaa ccaaaagaaa ttttagaaaa cctagaaaaa aaatctttag
     9721 tagattgttc tgaatctaca tacattggaa atcaaagaga tagtagtaat tttaatctag
     9781 aaaagttgat aagtaatgaa gttacaccta gaagtagaga ggaattgagt attgttgaat
     9841 acagagatgt ggtaaaaact attaacagtg cttatgagag tattcccatc agttcacaaa
     9901 cgattcttga gcttcatgga tacttatata aattttcatc tactagagga ggaagttata
     9961 agtcagataa tgattttatt gaacacaatt taaaacctag tgaatttata agtattgatt
    10021 caagtgtcaa taaagttgaa aaagctgtag aagaaatatg tgaagcttat aatacattga
    10081 tagaggaaga tgaaattgat atattaatat taatttctgc atttgtgtta gatttcatat
    10141 tgatacatcc attcaaagaa ggtaatataa aaatggcgag agtacttatc ctattgttac
    10201 taaataaaaa tgggtatgaa gttggaagat atataagttt aggaaagata tttgatgata
    10261 gttcatatga atattatagt aatttaaatt atttgaaagc ctcaataggt agtgaaaaag
    10321 cagatatgaa tgcatggatt gagtattttt tagagacgat attaactgca tatgagaagc
    10381 tggatgatag tttaaatata tctgataaga aaagacaaac aaaaactagt agaatagaga
    10441 aaataataaa ttctaccctt ggatatttta caaaagaaga tataagagat ttatgtcctg
    10501 acattccaga acctactata aatagagttt ttaataatct aagaaaacaa gataagatag
    10561 aagttgttgc aagaggaaga agtgcaaaat ggaagaaaaa atattaaaaa atgtttaaat
    10621 aactaaaatg gtatagtgtt ttacactata ccatttttag tgtcattagt atgagcaata
    10681 atttaacagt ttaagcaata gcaattttat ttattgatta tttttgaact gaaaaatggg
    10741 gttatatggt atactataat gataattaat tttagatgat aagatataac attacagaat
    10801 atcttaggag tatatgtaaa aaaatataat aaaagtgtga ggtttagtaa ttaacaaatt
    10861 atatggcttc acaccttttt ataaaataaa gatacttagc agaataatat tttagttgat
    10921 atgtttataa ggtggtactt gtttaggtaa aaataaatgg taaaaaagca gaagtcatgt
    10981 gtataaaaac ataagttaca ctctgtaata atagttattt tataattcaa caaaagaata
    11041 gaatgggtat atttgtgaaa taataatata aaataatctt aggagtgagc ttatgaagaa
    11101 aaaattatta tatataaatg taaattcaaa acctgaagac ttatcttcaa gtaaaactgt
    11161 agcaagaaaa tttataaata aatttatgga aaaaaacaaa aattttgagg ttgaagagat
    11221 agacctttat aaagaacata taccgagact tgagtatcag tactttgaaa agagaaatag
    11281 catagttagt gaagaaaatg ctaaaaattt agatgataag gatagaaaag agcttacaaa
    11341 gattagaaat ctttgtgacc aatttgtgag tgcaagtgta tatgtaatag cagcacctat
    11401 gtggagttta tcttttccag caccacttaa agaatatata gattgtataa ttcaagatgg
    11461 aaaaactata tcttttgaag gcaatgataa accacaagga attttaaatg atattgatag
    11521 aagtatggta tatatacaat cttctggtgg acacatacca tgggtgttaa agccggttat
    11581 gaataaaggg ttaaattatg tggaaagtat catgaaattt ataggaatta aaaaatttga
    11641 tgaattatta gtagatggaa ctggaactag tgaagaagaa agacaagctg caatagaaaa
    11701 agcatctgaa aaaatagatg gtataataga tgatatgaaa ttttaattat aatattttat
    11761 taaaaatata aaggaaaaac ctcttttaat gtagaaactt atattgaagg aggcgagaat
    11821 catgcgagaa gaaaaaagta atgaaaagta tgattgttat tggtgtaatc aagagaataa
    11881 tttctgtgta gaaataaaag ataatatagt tatgatagat gatggcactg gtacattaaa
    11941 acaagccgtt ttcataggat ataaacaaat ccaaattaat ttaaattgtt cacattgtca
    12001 aaacttaaat agaataaaat taaatttgta gaattttaaa atggaaatta ttgaaggaat
    12061 taaaaataga aagtgttaat atttagactg aaaattatat tttatacaat aaaagtttat
    12121 ttaaataaca ttgtatttta atatttattt tcagtcttac tttattaaaa ttattttttg
    12181 gttatgtccg gaaaatatct gtctactaat ttttgtgaaa tattttttaa ttggtcagcc
    12241 attgaagtca gacttaataa ggctatcaat aaatcatctt tattttctgg atttatacta
    12301 atattttcaa aattagactc tataaagtct ggtatattct ttctttgatt ttcttttatt
    12361 aacatgaatt tttcgtaaca tgaaactaaa tctttttcac tgaaattgtc cttttcatac
    12421 acaccatgta agattctttt aatttcttgt attgtaagtg tatttttcat agaataaata
    12481 attatcatct gtaaaatttg ttctttagta tacttttttc ccttaactgg tttgattaat
    12541 ccctctttgc tgtaattatt tatcatggtt ttagtaagaa ttttatcact ttcaaatctt
    12601 ttattggcag aaagcttatt gtcaattaga gaaataattt gatccatata taagtcaata
    12661 gagggaatat cgtttgaagt tatatctgca ttattcagtg tttctagtat tatagtatta
    12721 agttcattat tcat
//
LOCUS       DAEMAE010000105        12627 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEMAE010000105 DAEMAE010000000
VERSION     DAEMAE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.107"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(126..2006)
                     /locus_tag="KPH93_002263"
     CDS             complement(126..2006)
                     /locus_tag="KPH93_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG1689073.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVFGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASIKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISKNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGVASSVVDKIKSLI
                     EK"
     gene            complement(2217..8138)
                     /locus_tag="KPH93_002264"
     CDS             complement(2217..8138)
                     /locus_tag="KPH93_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG1689074.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRANVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDKISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEKQQEEVNNEQEEVNQEEVNNKDENNDKQQENIDKQEVNSNTNQ
                     DKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALETYADVQNSDIVINL
                     GKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEMNIPKINKISTSTAV
                     GNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTIEVRDIEAYGLYRVS
                     IQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEFQVVPDTKPEPPEGI
                     NVKGKFKSLDVSWKNHKQAKDFDLYYKEKDSNGSWIKYNDKLIRGTSSKIEGLKDNTT
                     YEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKENGGFGIPTNHIVDVV
                     YPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRGPIITFDKSYTMDTF
                     MATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNNNKYVMVRLKEPITA
                     TKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVELKEEVTQEKITELKD
                     RLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQEINKSGKYKDKNGQ
                     YTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYTQYYGESGAYKSGEI
                     NLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVAGATKIPHLNLNGTI
                     NDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSYGYSEKTGVLNTTDI
                     ESDKVTLNVPATAVYQGISKGNDDLNTQVDRLYNSMLAWEQIMDLVYSERGVFKTQDL
                     DGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHVGVEYGSVPGLMNGV
                     PFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLALLAQTFDDKTHSRL
                     EGSTMYKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGSMLKNNSDADLSNDT
                     FYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFKAWGVEITPELNSIM
                     DSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDGIQSGSLVKSNKISI
                     DLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKGNSKAKFTDNISPLN
                     NRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITNTVSDGDVRTENDSH
                     GPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEENPYIIVDMKETLPI
                     VGLKYTKPKTEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSNDKKNWEDVSQGTFEY
                     GQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIEVAELDIIGPPGDNI
                     EIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGSIVITGEYSGNPAFN
                     LPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKENFDSLSDKVKAELYR
                     YNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKARKATRNSIDISNKKV
                     ITVSNKVKGNIVRDK"
     regulatory      complement(8262..8347)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8519..9922)
                     /locus_tag="KPH93_002265"
     CDS             complement(8519..9922)
                     /locus_tag="KPH93_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG1689075.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10375..12270)
                     /locus_tag="KPH93_002266"
     CDS             complement(10375..12270)
                     /locus_tag="KPH93_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG1689076.1"
                     /translation="MLSNKKRSMAIVIAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSTNVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         3507 a         2327 c         1396 g         5397 t
ORIGIN      
        1 tcaaattatt atacatttta acctcaatat tattaaattg ttaagataat gaaaataaaa
       61 taagaatgca tttaaatata taactttata tctaaataca ttcttatttt ctatttactt
      121 aatatttatt tttcaattaa actcttaatc ttatctacta cagatgaggc tactcctcca
      181 cctacttcaa acaactttga tgaattttta ccttttaatg atgattcttg tgatttatct
      241 aacgtatttg tagcaagtat aactggtgaa ttgtttttag ctgctaatgg tccaactgct
      301 aaagcatcta taagtttgtc ttgatttgca actacaactc catctagttt agattgagag
      361 tagaatttat ctattacttt ggcattcgtc tcttgtctag tttgacctgc caccctattt
      421 tttgatatat ctttgtttgc tattttgcca acttgttcaa tagccttgtt agatattttt
      481 tctattccac caataaaata tatatttgac accttatttg atttaataaa ttgttcagct
      541 tcattatcta caccatcttt ttgtgtaagt actattggag ttttttcttt accagccagt
      601 gatgctattg ataatgaatc ggcttctcca tttcctgctc ctatatacaa cttgcttaca
      661 ttattcgtct tatctatttc ttttgctata ttaatagaag tttgatatct tgtatctcca
      721 cctattcttt gtactgatat ttttgaattt acagctttga tagcttcaac aactgaattt
      781 ggcattgagt tgtttccacc tatcacaatt acttttgatg gatttagcct tttcaattct
      841 tctacagttt tttgaggaag cttttcattt gatgataaaa gaatcggtga gttttttata
      901 ctagcaagtg gtgtagctgt aagcccatcc accatacttt tatcttctcc atttactatt
      961 acaactgtat ctgattttgt ccaaccactt tgagatattt tgatagctgt ctcaaatcta
     1021 tcagcacctt ttaatttttt gactgtgaca ttagatgtag aatcacttga atttcctcct
     1081 ccagagcttc caccactaga cgaaccgcct gaattacttc ctccaccatt atttccacca
     1141 ttgccattat ctggtggtaa ttcatccggc tttgataatt cattttcaac tatgtattct
     1201 aaactttttg gaagtgtaac ttcaacatat ttgtttattg tagcttttct tcccttagtt
     1261 gttttttgtg ctaatccaag ttttaatatt acatttgaac cactttcttg tgtttcttta
     1321 ctaacaatag aatagtttat cctaatattg tttaataaat attcaagtgc ttcacttttc
     1381 tctaccttat acttttcagg gtcattttta ctagaaccag ttccaccaat cggttttact
     1441 tcactctcat taataagggt aaatattaat ccttcatcag agtttatatt ctcataatcc
     1501 actgttttgt tgttatatgt tacaattttt aaggctggtt tatcgcttgt tccttttcca
     1561 aaaactttat agtcggcatt tgagttactg ccagattttt ttacagatat tcttcctata
     1621 ttcaatctat ttccgtctag taaatcttca tttgatgtaa catatatatc taatgtagtt
     1681 gtattacttg atttgttgta ttctgctcta caagtagcat ttgcatttcc actttttatc
     1741 tgagatttta tattatcaga ccaagtaatc ttttctttct gctcattatc tactacctca
     1801 acctcacctt gtacttttat actggcttga aatgaatata ctatgttttc tactgagtcc
     1861 acagatatat ctatagtatt tactttctca tacgtttttg ttccttgtaa aactggagct
     1921 gcatatgcac atgtagcact accagttaca gctactgcca tagttgatac tgctatagtt
     1981 cttataaaag attttctttt actcattaac acccgtcctt atctatcatt aactaagtgg
     2041 ataaatttat ttatccacct agcttttata tttaatttat aaattaaccc taaacttata
     2101 gttacattta tatactttac ttttaatatc tactatttaa aaactattct agtacctaat
     2161 catattaaat atacaatgtt agtattagta ttttcacgtt tttagagttc cacctactat
     2221 ttatctctaa caatattacc ctttacttta ttgcttacag ttataacctt cttattactg
     2281 atatcaatag aatttcttgt agcttttcta gctttactat tactatcagt aagacttatt
     2341 gtctttaact catcataatt cttagctcct acaggtacat acattgtatc acttacaaat
     2401 ctttgaccta ctggagtatc tccttgaagg tcattgtatc tatatagttc agccttaacc
     2461 ttatcactta aagaatcaaa gttttctttt ggcatatagt aaacccatgt acctttacta
     2521 atttcaccta attcaccttt ttctggaact tctgcaaata aaagtgcttc tccagatata
     2581 gtcttattgt ttttatctat aagtagtggt aaattaaatg ctggatttcc actgtattca
     2641 cctgtaataa ctattgaacc tgctggaatc ttacctgttt catcttcact tgtgctagag
     2701 tcatcacttt gatatacata atcttctgct aattttccaa ttccattagt acgagtttcg
     2761 tctgcattat tcattgtgcc aatttctata ttatcacctg gaggtcctat tatatctaat
     2821 tctgcaactt ctatattaga cttattattt gagattagtt ttacatatct agcttggtat
     2881 gtccaaagtt taccatcttt attaaatgta actctagctt cattatcttg gtcttttcct
     2941 ccaccaacta aatcttgtcc atattcaaaa gttccttgac ttacatcttc ccagttcttt
     3001 ttatcattac ttacttgtat cttgtaatta gttaaaggat tgtatgttgt gctagtattc
     3061 tttctaaagt taaataatcc ttttaatgag aacttcctag cttctgtctt tggttttgta
     3121 tattttagtc ctactatagg taatgtttct ttcatgtcta ctattatata tggattttct
     3181 tctacattta tatcagggtc tttttgaggg tctttgttcc actctgcttt acttattatc
     3241 tttcctttat atgtagtact tgcatcatta tcttttatat tatcaatttc aggattttgt
     3301 aatggtccat gtgagtcatt ttcagttcta acatcaccgt cagaaacagt atttgttatg
     3361 aatgaccatg ctgatttatt tattttccct tcatctgata attttacagt accaacttca
     3421 aattcttctg ttggatttag gtgataatca tatgcaacta ccttatatgt aaatgctctg
     3481 ttattaagag gagatatatt atcagtaaat ttagctttac tatttccttt tgcatccaca
     3541 aatccaactg ttctgtattt tacctgtgtt ccattttcac catctaaata atttccatca
     3601 cttcttatta tttcgtatcc tagtatctta tctttatcct tatctacatc taggtctata
     3661 gatattttat tagatttaac tagagaacca ctctgtattc catcagaaaa actagcactt
     3721 actttagtat cttttgccat actagctata ttttcattta aaatctttct tctagcttcg
     3781 tcatttacat attgtatctt tcttgattct ttttcgtagt tcttactatc cattatactg
     3841 tttaattctg gtgtaatttc tactccccat gccttaaagt aatctcttaa atctttttca
     3901 acaacacttg atgccattgc tactagcatt tggtcttttg gcaaactatt aattgggtca
     3961 ctacttgaaa gtgctctgta ttttcttgaa atctttgcat agaatgtatc attacttaaa
     4021 tcagcatctg agttattctt taacattgaa cccgtaaaat catcatcata agctagatgt
     4081 aattgccata acattccaag cctttcaaat acattgcttg gtatacctaa gctatttgaa
     4141 gttacatgct catatatctt atacattgtt gaaccttcaa gtcttgaatg tgtcttatca
     4201 tcaaaggtct gagcaagtaa agcaagtaca ttgttactag tttcactata tgtcatcttt
     4261 ccaatatctg taacatgtcc tatctcatga cctattcccc acccaaataa gtttcctcct
     4321 gtagcttttc cactatcatc tatttggaaa ggtactccat tcataagccc tggaactgaa
     4381 ccatattcta ctcctacatg taatccagaa gcatacataa atgctccaat aaacattctt
     4441 tggtatttta tgttcatacg agattttgga gccatatcat ttttagttaa gttaaattca
     4501 ttatcatcta cagttccatt accatctaaa tcttgtgttt taaatactcc tctttctgag
     4561 taaactaaat ccattatttg ttcccatgca agcatactat tatataatct atctacttgt
     4621 gtatttaaat catcatttcc ttttgatatc ccttgatata cagctgttgc aggaacattt
     4681 aatgttactt tatcgctttc aatatctgta gtgtttaaca caccagtttt ttctgaatat
     4741 ccatatgaat taccaacatt tgttcctgga taatatggtt ctccttttac agcatcttta
     4801 tatttagcta attcttctat atatgctttt atctttttct tagattcaga aactcccgca
     4861 ggactcttat catttattgt tccatttaga ttaagatgtg gtatcttagt agctcctgct
     4921 acacgtactt ttatatctcc tccacttgga gtatcttcag ggtatcttat gtataaactt
     4981 cctccacatt ctatgtctaa actatgaagt ttgcttaatt gaattacatt ttttccagtt
     5041 ttaagattta tttctccact cttatatgca ccagattctc catagtactg tgtatatatc
     5101 aacttaggag tttttccact tttacttcca acatatactg tgatttcatc ccctgctcta
     5161 gcagctactc ctaatgcctg ccagtcattt tgcataccta aattattgga atttacagta
     5221 tactgtccgt ttttatcttt atattttcct gatttattta tttcttgatt tacagtagtt
     5281 acatcagtaa gtgtggctct atcattatat aagtcttgag ctaattttaa ttctctatca
     5341 ataactgttt tatatggatg atattctcca ctagctttat ctggagtatt taatctatct
     5401 tttaattcag ttattttttc ttgagttacc tcttctttta attcaacttg taggtcatcc
     5461 ataaatagat ttcttacatc atcttctata ctgtcatact cataaaactt aagttctgat
     5521 atattagact tatatgaata ggatgagcaa acacttactt gtattttagt agcagttatt
     5581 ggttctttta atctaaccat tacatactta ttattattta cagggtctgt ctttgtttca
     5641 aaaggaacat cccccagatt gtgtaattga ccattttcat cccaatatcg tacctcaaag
     5701 tcatttattg tcttgtcaga aacatcaaaa cgaggtgtag ccataaaagt atccatagta
     5761 taggatttat caaaagttat tattggtcct ctattcgttc cctcagctgc attatacgtt
     5821 gggaatgtcc aatgtgttgt aaagtcccca tctactacat tgaatttatc aaatggattt
     5881 ttatcactat attcactttt gtcatatcca tctggataaa caacatctac aatgtgattt
     5941 gttggtattc caaatccacc attctcttta ggagtattaa ttaatctata gttaggtgtt
     6001 gttggtgcat ccatgctttc agttgtacct atatatgttt tggacattcc actagttccc
     6061 aagtggttag tagctgtcat tctaacttca tatgttgtat tatccttaag accttctatc
     6121 tttgaacttg tcccacgaat aagcttatca ttgtatttta tccaagaccc attagaatct
     6181 ttttctttat agtacaagtc aaagtcttta gcttgtttat gatttttcca gcttacatca
     6241 agagatttaa atttaccttt aacatttata ccttctggtg gttctggttt tgtgtctgga
     6301 acaacttgaa attctaatat actatctggg ctaaattgta taggtttata gtttgaatct
     6361 acattttctg gtactccatc taagttttct ggtgttgcat ctttataacc actagaccaa
     6421 tctccattta aagattgtat gctaactcta tatagtccat atgcttctat atctctaact
     6481 tctattgtat ttttactagt ttgtaatttt ttagtagata atacttttcc cttactatca
     6541 attttatcta gtataagttc gtatgctgat acatttactt cattattcca ataaaaagat
     6601 actttttcat ttccaacagc agtgctagtt gatattttat ttattttagg tatgttcatc
     6661 tctggctttg gtatttcttt atatacattg tttaaaaatt caacttttga tatttctgca
     6721 agatttctat cgtttgctgt tgtagctgtt atttttatag ttatcttttt tactgctact
     6781 tgtttaccaa gatttattac tatatcacta ttttgaacat ctgcatatgt ttctaatgct
     6841 gacctactat atgtagatgt tgtagagtta tcatctttac tgatattacc ctcttctttt
     6901 ggagtatctt catctgaatt aggtttatcc attgatgaat tttctttatc attattttta
     6961 tcttgattgg tattagagtt tacttcttgt ttatcgatat tttcctgttg tttatcatta
     7021 ttttcatctt tattatttac ttcttcttga tttacttctt cttgttcgtt atttacttcc
     7081 tcttgttgct tctcatcttt taagtttttc atatattctt tgttttcata aggaatctgt
     7141 tgaacattac catctttgtc agtaacctca actattcctt ttgctggagc agtaccttct
     7201 ggagctttta tattgatgac ttccatatta agtgcttctg ataaagctat atccattact
     7261 gctggttttt cctcagatat tttatcattt tttgaagata ttgtaactcc attattttct
     7321 ccactgaata aattttctac attaccatta atatctacat cactatttcc ttttaattca
     7381 accttacttg tatctactat aggattggta gatactatac tttctaggct ttttgcatct
     7441 tcactttgtc ctaaattttt ctgtacataa tctaagtcaa caatatctac ctttccatct
     7501 ctatttaaat caaattctct tttatttgaa tctatattct ctaaaacctt attataatca
     7561 ctcttatcta ctttaccgtc tccatttaca tcacccatta agaataattc ataatttaaa
     7621 ggtttatttt tattatctac atttgctcta ttgcttaata agactctttg tgaataactt
     7681 gatatatcaa tgtctttaat ttctttgttc ttaaatccag ctccagagac ttctatattg
     7741 tattttccaa caggtaaatt attaaataca atattataat atgatatatt ctcatcttta
     7801 ttttttattt cacttctact tgcagataat ttttttatag tatattctat attatctgtc
     7861 ccaacattta ctgttcctga atctttttct ccaagcttta ttattccact attgttacta
     7921 tcatctttta atcgtaactg catattagtc atactttctg actcgctatt ttttattggc
     7981 agtgaaaaat ttaaatctac ttcaagattc ccccttaact tttctgtaac tgatttttca
     8041 actgccctta gcttcccctc taatataggt tctgcaaaaa cgtttagtgg tgcactacac
     8101 atagatactg ctagtacaca agatattgcc tttttcatga aacaacaatc ctcccttaat
     8161 aaatatctaa tttttcatga atttaaatac tataagtttc agacaataaa aaaagacact
     8221 caaagagaat cttaattata aaataaaaag actttactct ttttagcaac tggctgatat
     8281 ggctttaaaa gctttaatcc ctatagtttt gcgtcattag atttccctaa ttttgccatt
     8341 taattttcac tactttctta cccattttat actatattta aactttttaa tacaattttt
     8401 ttaaattaaa ttataaaaaa ataacattta tattaattta ttaatataaa tgttattttc
     8461 cattataatt taattattta tatagcttcc ttacaagcat aattttgtgt atattttctt
     8521 attctttata tcttccataa aattcatgtg tagcatatag attcttatcc cctgttatag
     8581 ctataccaaa tcccattatt tcataccctt tatgcaacat attagtgcct tcttctggat
     8641 ttgtcttcca ctcattaaaa ataaaatttc ctatatcatc tgcatctcta tttgtatata
     8701 caggtttatc tggaatttta tatttaatta tattttcacc ttgaactgct atatatccag
     8761 gtttaatctc actccaatct aagtatttca aaaaagtaga atatgaattt tttccattta
     8821 tcgtatgaga taaagtcttc ttatttgcca ttaaattaga ccaatcattt gctagaccct
     8881 ctaatctact tgctacagat aacgctctta ctccattctc ttttctatac tgctttgtaa
     8941 gagcatatac tttacccttt attctctctt taaatgctct atcatttgga ttgtaatata
     9001 aaccttgttg atttggtgaa gcttgattta tacattgctt gataacattc tgatttatgc
     9061 ctcctagtac agtcaatttg tctgcaccac ttaaaatact cttgttactg ccattttcaa
     9121 ctaaaactac aggagcattt tttgctattg tagaggcagc taaagcgtct gttaattgta
     9181 agcctttact tagataaaat tctttactat ttttataaaa ctcttgtata actatcttat
     9241 tagtttcaaa tctatctgtg ccacccaatc taatagaatt tgtgctctta actaattcgt
     9301 cgctgattat ttcactagaa ccaagcgcat aactctttaa gcctgtcgtg tcaaatccca
     9361 cactatttcc atctgtaagt attataggta ctccatctct agcagctact ggtgatgcac
     9421 ttatcgcatc tgcttctcct gtaaatccat tggctataat tactttatct atcggcttaa
     9481 tttctttgat tttttcagct atttttatac ttgtatcaaa tctattttca ccaaatattc
     9541 tttgtacaac cttctttttt gatagtaact gattctcaac atttttactt attgttgatt
     9601 ctgagcctat tatatacaca gtctttgctt tgtttaatct ttccatagtt gtgtttggaa
     9661 tctggttttg ctttgtcatc aaaataggcg catttaaggc tccagctaaa ccacttgcac
     9721 ttagaccatc tgctaagcta ttatctgagt tgattagtat tgctgtattg tagttttgct
     9781 tatctgctat ttttgctgct gtctcatatc tgtcattccc ttttatttgc tcaactgatg
     9841 tcaacgcatt taccatagga gtatttacca ttactaaaaa tagtgtaagt cctatagcta
     9901 atatcttttt actattcttc atattatttt ctacccccaa tgtacttttc acattttttc
     9961 ttagtactca tacttatttt taatcaaaag ttacaatttt gttaccatat tttatatatg
    10021 ataccatagt tatccataca actcaaacaa tatacctaaa gtttttattc tctataaatt
    10081 caaaacatta taatttattt tatttaatta agttataagt tcaaaataat ttatgttaat
    10141 aagatgttta atcattataa gaagtatctt tttataataa tttttgcaat atgagtttct
    10201 caacattata atcaaaaaat aaaataaggt atctcaatct aattctaaat gagacacctt
    10261 atttttattt tgttatttgt atattttata atatctacac tatgtcttac atattaacac
    10321 attgtataat ctatattatc taaataaact tttaatatta attcctatat cttattagtt
    10381 cttagtaaat aaatttattt tttctgctat tttttcaata gcgtttttag ctattccttc
    10441 acctatttgt actaacttag tagcattctt aagtcttaac tcaactactt cttcttgtga
    10501 tttagaaagt tcttctgtag ttaatactat tggagctttt gaactagctg caagtggtgc
    10561 tgctgtaagt gcatcaacta attgtccatt tccacctaca taaccatctt ttgctacata
    10621 tgctttagtt gcacctgcat agtacttatt gattacttta gcattagtat cttttctatt
    10681 ttctccagct aatctttcaa ctttattatt taaatctata tcaactaatt tgtcttcaac
    10741 ttcttttgat acagaatttt ctccacctac tatttctaat tcttttcctt ttaataactt
    10801 ttcagcattt gcatctaatt cgttacctgt aactattatt ggagcaccaa attgagcagc
    10861 tctagctgat atactcatag catcagcttc tccgtttcca cctactacaa aagcagtctt
    10921 aaatgtatct tttaattgtt ttgctatttc taaagaagtt tcaaatctat cttctccaga
    10981 aactctctca actttactta cacctaattt agaaagtttt tcttcagttt cttttgatac
    11041 tttagattct ccacctacta tatatatttt cttagaagat agattacttc caagtactct
    11101 taatatttca gcttctattt ctgatggtag tgaatctttt ttagataata atattggtgc
    11161 atttttttga gaggcaagtg gtgctgatgc taatccatca actatagcat cttctccaac
    11221 taaaattata gcttctgctt ctccatcttt gaatctctct ttactaactt ctactgctgt
    11281 tgtaaatctt gaatcaccag ataaaacttc tacttttaat tcttttccat cttttacatc
    11341 agttaatact ttgtgtaagt ctctaactaa cttatggttt ttacttgaaa cacttattgt
    11401 ttctggcttt tcatttgcct tgcttaaaac tatatcaaaa cttgattttt ctggcttatc
    11461 tttatcatca ctatcttctt gttcttgagt aaggattgct tcattttcaa atgttaaagc
    11521 atatccattt ttaactttat taactatttc tcttcctaat gtagttaatc taattccatc
    11581 aaataattct tctgctgata tgtcttgtaa atctgcgttt ttaactgtta tcgtatgtaa
    11641 ttcttttgaa tctatgtcac tataactctt ttcataacct tcaaaagtac cttcttctga
    11701 tgttatcact tttgagaaat ctaaatgttg gtctcctaca tttaatgtaa taacatttgt
    11761 tgaatctttt gctcttttta cttcaactgt atttgtagat tttatagttg ctgttaactt
    11821 gttatcagaa tcttctgcta aagttgcatt gtaagcatta actgcatcta ctatttcttg
    11881 agcagttttg tagttttcaa ttttatagtc aactactttt ccacctaaca cttgatgacc
    11941 tttatcttga atagtaactt ttaaactttg cccttctgtt aaagagttta ttttattttg
    12001 taattgagtt gcattagtta agtttacatt gtctactttt atatcgtaaa ctctatcatt
    12061 tacatttgct cctgcttttg tatcttcaaa cttaacttct aatgcttttc taacctcttc
    12121 gattagttta gcagaatctt tagcacttac tgtgtaattt ttatcaacat tttctgtcac
    12181 agtattgtct gcgaatattg gtgctgctgc actcataact gtagcacctg ctattactat
    12241 tgccatactt ctctttttgt tgcttaacat atttatgtcc tccgaatatt attttttatt
    12301 ttttatattc gtatttccca cacataaata atattatata taatattatt tttcaatata
    12361 ttttatgtgt ttacataatt tactaacaca aacactaata atatcttctt gaaccccctc
    12421 cccacttagt tgtctataat catgctcaaa gatagaaatc cctattttat agtgattttc
    12481 taataaacaa tcatatacta acaaaagtat ttaaactcca tttttatata ctactataca
    12541 ctaatttttt ctttactctt attacttaaa tatatatcac taaattaata tctatctcca
    12601 tatattaaaa tatataacat attataa
//
LOCUS       DAEMAE010000106        12574 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEMAE010000106 DAEMAE010000000
VERSION     DAEMAE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.108"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..497
                     /locus_tag="KPH93_002267"
     CDS             132..497
                     /locus_tag="KPH93_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689077.1"
                     /translation="MNYHTKEELMEALRVVSSSIINCEKVQPKFAEGTSQHSLLRNRI
                     KALHISKALIESEDIIDKYTKEELIEALPPITSIISKCEKGQKKFSEETSHHTRFKNI
                     IEAMYISKSLIMDEISKRD"
     gene            756..1955
                     /locus_tag="KPH93_002268"
     CDS             756..1955
                     /locus_tag="KPH93_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG1689078.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVISVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIHQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNILVIKKIYDDEDLKYVNKIANKITEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            2007..2510
                     /locus_tag="KPH93_002269"
     CDS             2007..2510
                     /locus_tag="KPH93_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689079.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIVKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            2617..2934
                     /locus_tag="KPH93_002270"
     CDS             2617..2934
                     /locus_tag="KPH93_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689080.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            3092..4051
                     /locus_tag="KPH93_002271"
     CDS             3092..4051
                     /locus_tag="KPH93_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG1689081.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRTVNLIPFYTIYQFIVDSNIDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     TIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGITIYINMNKLFPNDIKRFKVII
                     ATSLILGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSSNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            4166..4951
                     /locus_tag="KPH93_002272"
     CDS             4166..4951
                     /locus_tag="KPH93_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689082.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTVLEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGIMSVLIIRFIHPFIKDFVGIIPYRILISMSIITTLWTILDLIVT
                     VITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALLKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            5243..6562
                     /locus_tag="KPH93_002273"
     CDS             5243..6562
                     /locus_tag="KPH93_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1689083.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6637..7065)
                     /locus_tag="KPH93_002274"
     CDS             complement(6637..7065)
                     /locus_tag="KPH93_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689084.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYTKN
                     RRKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(7156..8532)
                     /gene="hydG"
                     /locus_tag="KPH93_002275"
     CDS             complement(7156..8532)
                     /gene="hydG"
                     /locus_tag="KPH93_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG1689085.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(8533..9618)
                     /gene="hydE"
                     /locus_tag="KPH93_002276"
     CDS             complement(8533..9618)
                     /gene="hydE"
                     /locus_tag="KPH93_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG1689086.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLTLDEILE
                     CCDIGYKIGYRTFVLQGGEDTYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSSRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9654..10862)
                     /gene="hydF"
                     /locus_tag="KPH93_002277"
     CDS             complement(9654..10862)
                     /gene="hydF"
                     /locus_tag="KPH93_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG1689087.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIRVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYIKGI
                     LERSLELFNY"
     gene            complement(10877..11128)
                     /locus_tag="KPH93_002278"
     CDS             complement(10877..11128)
                     /locus_tag="KPH93_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG1689088.1"
                     /translation="MKKVAVISAILEEPKECQLKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEI"
     gene            complement(11509..12549)
                     /locus_tag="KPH93_002279"
     CDS             complement(11509..12549)
                     /locus_tag="KPH93_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG1689089.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLESKKDFKNLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTIITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         4622 a         1552 c         1917 g         4483 t
ORIGIN      
        1 gtaatgttac tgatagttag aatatttaag tgttaaaaga tgtgtataaa taataatgat
       61 gtcagttgat aatgatatta aattgaggta tactgtttct ctaaattaaa aaataaagga
      121 gtaaatgtgt tatgaattat catacaaaag aagaattgat ggaagcactg agagtagtat
      181 cttcaagtat tattaattgt gaaaaagtac aaccaaaatt tgcagagggg acttcccagc
      241 attcacttct tagaaatagg ataaaagcat tgcatatttc taaggcattg atagaatctg
      301 aagatattat agataaatat acgaaagaag aattaataga agctttaccc cctataactt
      361 cgattattag caaatgtgaa aaaggacaga agaaattttc tgaggagaca tctcatcata
      421 cacgttttaa gaatattata gaagcaatgt atatttcaaa atcattaata atggatgaaa
      481 ttagtaaaag agattaaagc ttattttgca atttaacatg ttttaatata cattttatat
      541 ataagaaaat aagtaagcat acatgttata aaatattgga acaatatgca attataaatt
      601 tgttaacact aatatctata tgttaaaata tagtagaaga tgttagtaaa taataggatt
      661 agttctattt taatacaatt aaattaagct tggcttgaaa tttgtattta aaaagtaaag
      721 tagtctataa ttaagaaaat ggagatagaa ttataatgga aaagttatat tatacaaacc
      781 agtatataag agattttaca gcagaaataa ttgaaataaa agaagtagaa aataagtatc
      841 atgttttatt agataagaca gctttttttc ctggtggagg aggtcagttt ggagacctgg
      901 ggaaaataga gaatgatgat gtaataagtg tgtatgaaga tggaaaaaat atataccatg
      961 tattagataa aaaacctata aagatacata aggtaaagtg tagtattgat tgggatagaa
     1021 gagaagatgg aatgcatcaa cattttggac aacatgtgtt atctggatgt ttttttaaga
     1081 ctttcaatgc aaatacgaca ggttttcatt tgggaaatga gtatagtaca gtagatatag
     1141 aaggtaattt agaggaggat aaaattagag agatagagtt gtttgccaat gaaattattc
     1201 atcaaaatat acaagtagag acattacttc caacgaaaaa agaactaaaa aagatttggt
     1261 taagacgtga tttgccaaat actgatgaag agataagagt aataaaaata ggtgatttag
     1321 atacgaatgc atgttgtgga gtgcatccaa aatcaactgg tgaattgaga atgataaaaa
     1381 taaagaaatg ggaaaaacat aagtcaagta caagaataga gtttctagca ggtaaaaggg
     1441 cagtggatga agtattgaag agagatatat atttgactaa aatatgtaga tatttaagct
     1501 gtagtgagga agatgctata aatggtattg taaatctaaa tagtaaagtg gaagaggctt
     1561 taattaaaaa aagaagactt gaagaagtag tatctacata tcaggtcaaa gaaatgattg
     1621 aaaaagcaaa caaggttggc aatattttgg tcattaaaaa aatttatgat gatgaagatt
     1681 tgaaatatgt aaataagata gcaaataaaa ttacagaata tgaaaatagt attgcattat
     1741 tagctgttaa atttaatgac aaaataaatt tagtttttgc atgttctaaa aatttaaaag
     1801 atattaatat gggagtttta ttaaaggatg caataagcct tatagatggc aaaggtggtg
     1861 gaagtaaagt attagctcaa ggaggaggta agaataatgg aaatttagaa gctacacttg
     1921 actatgcatt tatgaagata gaaaaaactc tataaatata ttatataaaa aaccttaata
     1981 gataagatag atatggagga ataagtatgg agcaacaaaa ggactacata gagttatatg
     2041 aaaatgaaga attaatagag ataagagaag cacgtatgga tgatttggat acaatagtaa
     2101 aatttaatta taatttggct aaagaaactg aaggaaaaga acttgatatg gatgttttaa
     2161 ctaaaggtgt aaaagcattg ttattagatg agagaaaagg aaaatatcat gtatatacag
     2221 tatttgataa agttgttgca cagattatgt atacatatga gtggagtgat tggagaaatg
     2281 gaaatttcct ttggattcag agtgtatatg tagataagga atatagaaga aaaggtatct
     2341 ttaattattt atttaattat ataaaaaata tatgtgataa agatgaaaat attgttggaa
     2401 tgagattata tgtagaaaaa gaaaacataa atgcaaaagc aacatatgag tctttaaata
     2461 tgtatgagtg tgattataat atgtatgaat acgaagtaat acattcgtaa tataaatttg
     2521 taatacagtt gtactaatgt tataaagcat tgattatatt gacgattata tgaaataaac
     2581 tggatattat aattagaatt tttagggggt ataaaaatgg gcgttttaaa aggaatgttt
     2641 tatgtatttt tgtttttaga tttagtgtca atattctgtt tcttttttaa taaaggaaaa
     2701 atagcatcta ataaaattgt gtttaatgca ataggagtac ttacgtttgt actttgtttt
     2761 atgcttttta gttattatcc taataataat cttattggaa aatttattgc ttctctattt
     2821 tttatatttg gagttgcagg agttatgttg aaagaaaaaa attttttata tgctagactt
     2881 cttttaactg tagttatagt tttttctaca ctaagattgt tcgttattca ataacatgta
     2941 ttcgtatgaa aatttgttaa agtctacaca tttaagtgta ggcttttttg cttttttatt
     3001 taaaaattaa ggaaatttta aggaaatttt aaaatcgtct taaatattta ttgatatatt
     3061 taaaatatta atagcgaaag gagacttatc agtgatagaa agtaaaaagt cagaaaaatt
     3121 gttactgtca attatataca taatattttt ggtatatatt ttaatgatta taagaataat
     3181 tttatttaga gatgtaccaa tttacgctat tttcaaggga acttttagga ctgtaaattt
     3241 aattccattt tatacaatat atcaatttat agtagatagt aatattgact ttatgaaagc
     3301 tactgtaaat attatagaaa atataggtat atttattcct atgggaattt ttttacctat
     3361 agtatgtaaa aatctaaata aaaaaactat aattataaca attatacttg ttagcttagc
     3421 atttgaatta acacaatata tttttgctct tggaagtagt gatatagatg atgttatttt
     3481 aaattcatta ggtggcataa ttggaattac tatttacata aacatgaata aattatttcc
     3541 taatgatatt aaaagattta aggttataat agctactagt ttaattttag gagttatagg
     3601 attaggagtt atttctaaaa attatcataa cttactaact tttaaattta gacctgatag
     3661 aaaaatatca aaaatattaa ttgaagaaaa tagggaaata attaaggata taaataaaga
     3721 tacagtagac atagttggga catttgagag ctttaaaaaa ggaataataa ctattaaagc
     3781 aggctctagt aacaaagtaa agagaccaga gaatttaatt gattctgatg gaaatataag
     3841 aatttattta aatgaaaata caaaattagt atcttatata attaccaaag aatcaaaaat
     3901 ggatatagtt aaatatgaag aatttgatgt gaagaattta gatttattaa ggagatatga
     3961 tactattaat gtttggattg ataaagaaaa tcagccaaag gataaacatg gtattatggc
     4021 tagtaaatta ttaattggat tgtatgaata aaatattaaa taagaataaa agaataacaa
     4081 atataaaaaa cgaaaaaact atttctataa ttatttttac gttgtgtata aaataaggac
     4141 actggtgaag gagtagagat aaattatgga gataataaaa tatatcttgt acttttttat
     4201 atattcattt ttaggatgga cagtagaaag tataggatgt tctatagcaa gcaaaagaat
     4261 aatcaacaga ggttttttaa atggtccaat atgccctgtt tatggttttg gagcggtaat
     4321 tgtgatatca ctattgggaa gatttaataa tgtagtaata gtatttctat taggaatgat
     4381 tttaactaca gtactagaat attttacagg atttatttta gaaaccttat ttcatgctaa
     4441 atggtgggat tattctgaca gaaaattcaa tataaagggt agagtatgtt taaaaaatgc
     4501 aatatacttt ggtataatgt ctgttttaat aataagattt atacatccat ttataaagga
     4561 ttttgtaggt ataattccat atagaatatt aatttcaatg tctataatta caacactttg
     4621 gactatactt gatttgattg ttactgttat aacacttaaa aaattagata taaagttaaa
     4681 cttactagac gatataatta cagaccttaa tgatataaac gtgaaattgg ataagtttga
     4741 tagaggagaa atacaagctt tacttaaaac aataaataac gatgaattag aagttagaga
     4801 aaaaataaac aaaataaata gtaagttgga ccgtatagag tctaatatta ttttacaaaa
     4861 aagagttata aaagcttttc cacatataaa acataaaaaa caacaagaac aactagaaca
     4921 ttttaaaaaa attattaatg agaaaaggta attatacaat ttaatgaaaa agaatagaat
     4981 aatggtattt ataagatgat tttcagagga ctctataaaa atagaataga gtcctttatt
     5041 ttttgaaaaa taatgtttat ttgataaaaa taaaatatat tgtttctgaa taacaaatat
     5101 tttgaaaatt ataaaaacat atattgacaa tttgtgtcaa aaatactaac attaagtata
     5161 taaagaattt tatatgagaa tatctttata aatcgcaata attttaaaac ataaatttat
     5221 ttaaagaggg gatgatttgc caatgagcga gataactagt caaatgataa gtacagaaga
     5281 aaaaaaatat gttgctaaaa ctcaaaagat accttattat ccagtagcat ttaaatcagg
     5341 tgatggtgca atgctatatg attatgaagg taatgaatat gtagattttt tagcaagtgc
     5401 aggctctgca aatgtaggtc atggtaataa ggaaatctca caagcagtta aagaacaaat
     5461 ggatgatata acacaatata cacttgcata tttccatagt gatcctcctg taaaattagc
     5521 tgagaagctt gtggaaatag cccctggaga taatgataaa aaagtactat acagtgcaac
     5581 tggttctgct tgtatagatg ctgctataaa attagcaaga gggtatactg gaagaacaaa
     5641 gataatatct atgtgtgaat cttatcatgg aagtacttat ggagctatat ctatatctgc
     5701 tttaagtaca aatatgagaa gaaaaatggg tccattgtta ccagaagttt atcacttcca
     5761 ttatcctgat aaaaatagaa cagctaaaga atgtttagat gagatagaat atgcttttgc
     5821 acactatcta ccagcagaag aggtagctgc aatatttata gagccaattg caggagatgc
     5881 aggaataata gtgcctccag tagagtgggt acaaggttta agtaaaatat gtaaagaaaa
     5941 tggaatatta cttgtaagtg acgaaataca acaaggtatg ggaagaacag gtaagtggtt
     6001 tggtatagaa aattttggtg ttgaagcaga ccttatagtt cttggaaaat ctgttggagg
     6061 tggattacct ctaggagcag ttgttggaag aacagaaata atgcaaagtc ttgatgcacc
     6121 agcacattta tttactttag ctggaaatac aacagtgtgt gtggctgctt taaaatctat
     6181 agagataatt gaaaaagaga acttacttca aaagagtata gaaatgggag attatattaa
     6241 agcaggattt gaaaaactaa aagaaaaata cgatataatt ggagaaataa gaggaatagg
     6301 attatctata ggagttgata tagttaaagg aaaaggttct aatgaaaaac atccagatgc
     6361 tacagctaag atatgttata gatgtataca aactggatta ataatgatat tcttaggaca
     6421 atctacttta agagtgcaac cacctctagt aataactaag gagcaagtag ataaagcaat
     6481 gaacatcata gattctgcaa tagatgatta tttaaatggt agaattggtg atgaagtata
     6541 tgaagtaact caaggttggt aaaataaata ttaaatgaaa ataggagtta tctcaatttt
     6601 agaaagtaat ttctgaaatg agataactct tttaagttac ccacaaataa gaaaattgtt
     6661 tgattcatta aagactatat ttcggatgtc gtttttgtga gtatcagaga cagtattacg
     6721 agaataatta cctttattcg tactatctgt taatttagtt ataattaaga tagctaaaag
     6781 aataagaatt actgtgtata aagcaataac atatggtttt cttttatatt tgtatttgtt
     6841 attgaaaaaa gaatggccat aattattttt atttacttta gatgtatttg tattcttttt
     6901 cttgttacct ttctttttat catcctttct tctattttta gtgtaaggaa tgactttgtg
     6961 tgtattatca agtttgatat ttttattttt ttgaatattg ttttccaggc tagctttttt
     7021 ttctccaaat tgaatagttt tattggtatt gttgttatct ttcaatgtta atctccctcc
     7081 cttaaacttt ccctttagat aatattacta ctaacctatg gaatatacaa taaataatat
     7141 ataagtaata aaattttata ggtataaatc tctttctcca cgctctaagg cagtcatatt
     7201 gttagatact atgtttctta tatcttctct ttctatctta cctatttctt ctgcgataag
     7261 ttttttaccc ataattttaa gttcttcatc tccataatcg attaaaaatt catttaatgt
     7321 agtaagagca tttggtgtac atacattatg gatttgtcga cttttagcta agctcataaa
     7381 tctatcacca gttctaccct tgcgataaca agctgtacaa taactaggta tatatccatc
     7441 agtaatcagt tctttaagca cctcaacagg gcttctatgg tctccaactt cgaattgttc
     7501 tgtattgtca ccatcttcat atgctttata tcctccaaca cctgttagag aacctgcact
     7561 aatttgtgat acaccatatt ttagtaactc atttctcatt tcggcagttt ccctagtgga
     7621 cattatgatt ccagtaaaag gaacagccaa tctagttata gctactattt ttttgaacat
     7681 atcatcagaa actaagtgag gaaaatcttc taagctcata ccttcagctt ttttcaatct
     7741 agggaaagat atagtatgga aacctacacc aaatttttcc tctaagtgtt cgttgtgcat
     7801 cataagtcct agaacttcaa atttagggtc agataaacca aataaaactc ctgcgccaac
     7861 atcatcaacg ccagcttcca tagctctatc aaaagctgtt aaatggtaat aatagtcatt
     7921 ttttatagat tgaccatgca tttttataaa tgttggtttg tgataagttt cttggaataa
     7981 tatataagtt cctattcctt tttcttttag taatttatac tcatctacac ttgtagctgc
     8041 tatgttaacg tttacccttc ttatatttcc gttttcatta tatgttgaat aaattgcatc
     8101 taagcaatct aagatgtaat ttatatcaca atttttaggg tcttcaccag cttctagagc
     8161 aagtctttta tgacccattt tttctaatat tttaacctct tcctttatct cttccatggt
     8221 aagttttttt cttttaaatt tgttgcattt actaaaacca caataaacac attcatttac
     8281 acagtagttt gatacataaa gaggggcaaa taaaaccact ctattaccat atatttcatt
     8341 ttttatttgc ccagctatac taaataatct atctagttgt tttttatctt ctacttcaag
     8401 aagtgtagct atatctttat aactaagttt ttcccttcta tcagccttat caagtacctt
     8461 ttcaatatca tcagaagttg aattttttgc atcttctagt aaagaattaa taagttcatg
     8521 gtttatgaac atttacaaac gcctccattc aatattatca ccacgagaat agtcaacttc
     8581 aagtcctaat gacttgatat tttcttctaa actttggtga tattcacaag cttcttgacc
     8641 agtaaaggct ttgttatcgt aaagagaata ttgttttcta aattcttgag gagaaaggtt
     8701 tggcataatt acattgcaac cagctaaaag acctgcattt ctactactag ggtttataga
     8761 tgccaaggct gttgtagcgg gtaataaaac ttttggaagt agaagtcttg taattgaaag
     8821 cataagtaat gtcttttcta aatctccatg tttataatcc ttaaaaattg tatcatggtg
     8881 tggaataaaa ggtcctattc caaccatatg aggatttaac tcttttaggt ataataaatc
     8941 acgaacaagg tcttcgttgt tctgaaatgg tgattcaacc ataaatccag cacctacctg
     9001 ataaccaatt tctttaaggt tttttaggca ttcttttcta gttctaagct caatgtttgg
     9061 cggatgtaat tttttataat gactgtctgt agcagtttca tgtcttaaaa gatatctatc
     9121 tgcaccacat aaaaagtatt ttttatacga atcataactc ttttctccta gtgatagagt
     9181 tatagcacaa tcctcatatt tattttttat acttgaaaca atttcacata ttttatcatc
     9241 agtaaaatat gtatcttcac caccttgcaa aacgaatgtt ctgtatccta ttttataacc
     9301 tatatcacaa cattcaagta tttcatcaag tgtaagtctg tatcttatga tatttttatt
     9361 agaagattgt attccacaat agtagcaatt atttttacaa tagtttgtaa gctctataag
     9421 tcctctcaag tagactctat ttccataatg tttatttctc atgtcgctag cttgtttata
     9481 aagatagttt cttaaatttt gttcgaagtt atattcctca ttgtcgatat tttctattaa
     9541 atatagtagt tcatcaaaac ttaatgaatt aatgctgtaa agtttatcta taaagtattt
     9601 aatcttattt ttagtcatat tcaaatatgt aattattaag tatagttaaa tttttaataa
     9661 ttgaaaagtt caagtgatct ttccaaaata ccttttatat atgctattaa gataccatag
     9721 tttacaatag gtacattgaa agattttgcc ttttctattc tagataacat tccagctcta
     9781 ttcatcatac aagcgccaca gtgaactaca agggcatatt tatttatatc ttcagtgaag
     9841 gatactccag aactaaattc aaaagtaata tttttgccag ttttctttct aatcatatta
     9901 ggaattttta ctgttccaat atcatcagtt tgtctatgat gggtacatcc ttcggccatc
     9961 aagattttat caccatcttt taaattttct aaagcataag ctccattaat caattcttta
    10021 aggtctccct tttgtctagc aaacaatata gaaaaagatg taaggggaat atcttttggt
    10081 gtatctttat ctacttgtgg aaatacttga gagtcagtaa taacaagttt tggttttttg
    10141 cctaaattag atagagtttc ttttaaacta tcttcttttg taactataga aattgcgcca
    10201 ttatccaaaa tatctcttat aacttgctgt tgaggaagta taagtctacc ttttggtgct
    10261 gctttatcta ttggaacaac taaaacaact aaatcatttg gttcaattaa atctgatacc
    10321 aatttgaact cagtgctatc tttaggcaaa actcttatta tttcattttt taaattttca
    10381 attccaattt tatctgttga tgagacagaa acaactggac atttaacttt atttttagtt
    10441 aaatttaaaa tttctgattg attcttaata gtatcaattt tgttaagaat taaaatatga
    10501 gggatattct tatcattaaa cttttcaatt agtgataaat cttcttgtga tataccaatc
    10561 tggcaatcca ctactagtaa agctatatct gttttttcta aaacatctag tgatttacct
    10621 attctaagtt caccaagttc accaacatca tcaagaccag cagtatctat aagaacacaa
    10681 ggtcctatag gaaggatttc cattggtcta aatactgggt ctgtagtagt tccagcaata
    10741 tctgagacta ttgcagcact ttgattcgtt atagcattta ttatgctaga tttacctgca
    10801 tttcttttac caaagagacc tatatgtact cttactgatt gtggagtaga atttagactc
    10861 atttgtactc accaccttaa atttcttttt ttgatatact agtttttaca gatacatgag
    10921 gtatgttgcc caatttacca gtcaaactat taatagtatc catttcgcca acaactgcta
    10981 tacatacgat tgatacgcct tcttcttcgc aaggaatgcc cattcttcct ttaataatac
    11041 ctttaaaacc tgatacaact tcattgaatt taagttgaca ttcttttggc tcttccaaaa
    11101 tagcacttat aactgcaacc tttttcataa atatcctcct aactcagaaa atatgtctta
    11161 aacaaatcca aaatcaaaaa acttttcttt gtaaagaaaa gtttctacaa aagaaaagtt
    11221 attaattaca caagtataat gtgctatatt aaactatttc cttacagatt agataactct
    11281 tttctggtag ggttagcttg taaaaattta atttaacaac aattaactga agaaaatttt
    11341 tatacaattt atataaatta taataaaaat taactaaaga aaattgaagt tacttatata
    11401 atagtagtaa attgaaatta agtctataga aaaaccaatt tttgtataaa aataaaaatt
    11461 aaagatagtt atttgttaaa aattgacttt gataaaatat ataattaatt attctttaag
    11521 catataattt ataagtaatt ttagtgtatt ttcaatagct tctatatgtg ttctttcata
    11581 atggtgacta ttgtttgttc caggaccaac acatgctatt tgaacatctt ctccccaacg
    11641 aatagcttct gaagcatctg aaccatagaa agtaaataca tcgacagcgt agtctaaatt
    11701 attgtcttct gcaacatcga caagcttgtt tcttaaatta aaatcatata atgataaatt
    11761 atcttttgca actatagtta caccatgttc actagatgtt tgacttggcc cagtaggcgc
    11821 tatatctatt gctacaaatt cttttgtttt ttcaggtaaa catttagaaa cgccatgtcc
    11881 aatttcttca taattactta tataaaagtt agtagtatgc ttaggtgtta aattgttttc
    11941 tttaaaatat ctacatattt caagaaccat tgctacagct gatttattat ctatatatct
    12001 agattttata aaaccagatt ctgtagcctc aaatctatgg tctaggcata caaaatcccc
    12061 aacacttatt cctaaatcta atacatcttg ctttgtaaat actttttcat caagtctaat
    12121 aatcattgtt tcttcatttc tttcagtggc agcttttgtt tgaccatata tatgagtaga
    12181 agcatactta aatacaacac ttcctctgat ttttttacct tttctagtta taatagtaca
    12241 gttttctccc tctatagctg cccaacatcc accaccaact ttatggtatt taagagtacc
    12301 atcagatgat atttctttta ctatagctcc aagtgtatcc atatgtgcag atattgttat
    12361 ttgatttgaa tcatcttcac caggaagagt agctataagt gcaccttttt ttgtaagaat
    12421 agtgtcaaga cctaaatttt taaaatcttt tttgctttct aaaactgcat tttcagtata
    12481 tccagctggg cttggaatgt ccaaatattt tttcattaaa tttatagtat tagttaaatt
    12541 taaattcata ttgtttcctc ctaaataaat atat
//
LOCUS       DAEMAE010000107        12382 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEMAE010000107 DAEMAE010000000
VERSION     DAEMAE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.109"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..1525
                     /locus_tag="KPH93_002280"
     CDS             56..1525
                     /locus_tag="KPH93_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG1689090.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCIQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SGFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     IDENKNNQNTNNQNTNNQQKPETPPTDNKPNETQPQPQA"
     gene            1590..2183
                     /locus_tag="KPH93_002281"
     CDS             1590..2183
                     /locus_tag="KPH93_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG1689091.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            2365..3711
                     /locus_tag="KPH93_002282"
     CDS             2365..3711
                     /locus_tag="KPH93_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1689092.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPKREITVADEEDLYYQAL
                     DLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            3893..4264
                     /locus_tag="KPH93_002283"
     CDS             3893..4264
                     /locus_tag="KPH93_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG1689093.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            4257..4946
                     /locus_tag="KPH93_002284"
     CDS             4257..4946
                     /locus_tag="KPH93_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG1689094.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEVEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            5467..6333
                     /locus_tag="KPH93_002285"
     CDS             5467..6333
                     /locus_tag="KPH93_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1689095.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            6575..7384
                     /locus_tag="KPH93_002286"
     CDS             6575..7384
                     /locus_tag="KPH93_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689096.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYIGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFVFSNGNEYVGYFEEDVMSGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            7528..8592
                     /locus_tag="KPH93_002287"
     CDS             7528..8592
                     /locus_tag="KPH93_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG1689097.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKINQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEIIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            8620..9339
                     /locus_tag="KPH93_002288"
     CDS             8620..9339
                     /locus_tag="KPH93_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1689098.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            9362..9946
                     /locus_tag="KPH93_002289"
     CDS             9362..9946
                     /locus_tag="KPH93_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG1689099.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDVVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSISHTNKGVFSGLKNPLNVTRYHSLIIDSNTVPKELEITATTDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            9948..11297
                     /gene="pabB"
                     /locus_tag="KPH93_002290"
     CDS             9948..11297
                     /gene="pabB"
                     /locus_tag="KPH93_002290"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG1689100.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVANDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIMDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIKCVNKRIETRPIKGTRPRGKDKE
                     EDLKLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            11287..12030
                     /locus_tag="KPH93_002291"
     CDS             11287..12030
                     /locus_tag="KPH93_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBG1689101.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAIDLNIHIERMISSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVCTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            12064..>12382
                     /locus_tag="KPH93_002292"
     CDS             12064..>12382
                     /locus_tag="KPH93_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689102.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDINYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRES"
BASE COUNT         5206 a         1145 c         2217 g         3814 t
ORIGIN      
        1 tagtaatata ataataggga tatataaata attttattgt aaggagaagt ataggatgag
       61 taatgtgaac aaaaagctag ttatagctat tggagctgta gcattagtag taataatttt
      121 tataggcata atgtgcatac aatttaaagg ggagaaaata gcaaaaaaca catatgttaa
      181 tggagtaaat attggaaaat taactaaatc gcaagcaaaa caggaattag ctaaaaaata
      241 caaattagag aatgttgagt ttaattacaa tgataaaagt tggaaagtaa agagcaaaga
      301 tttaaattta tctactgacc tggataaaac agtagaaaac gcatataatt taaatagaaa
      361 gagtggtttc tttggaaatc tatcaaaaac tatttcagca aactttggca aaaaaagcaa
      421 cttagtagtt gtgataaatt atgataaaaa taaattaaaa gcagaaatgg aaaaaatagc
      481 taaagaaata gatgtagatg taaaagatgc tacacttgat ataagtggtg aaaaagttaa
      541 agttatacca gattctgatg gtcttaaaat ggatatttct aagagtatgg agaattttga
      601 taatcaaact aagaaaggta actataaaaa tgagttagta gtaaaagcta ctccagcaaa
      661 agtaaaaaaa gagcaacttg ctaatataga tacaaattta ggtacgtatt caacaacatt
      721 taaaacttct caaataaata gaagtataaa cataaaatta gcaacagata atataagtaa
      781 tgtattactt atgccaggag aaactttctc atttaataaa catacaggga aaagaagtaa
      841 ggaaaatgga tataaaagtg cacctgtaat aatggaaggt gaaatggaag aagattatgg
      901 tggaggagtt tgtcaagttt catctacatt atacaattct gtgttatacg caggtttaga
      961 aattgttaat gtaaaaaatc atactatacc atcaagttat gttccaaaag gaagagatgc
     1021 aactgttgca gatagtggaa tagatttctt atttaaaaat aatctaaagc atccagttta
     1081 cataaaaaat tacgtttcag gaaatcaaat tgtttgcaat atatatggaa gtgcagaaga
     1141 taaacaaaat ataacaatat caacaaaatt agatggtgtt tcacaaacta caatgaaaag
     1201 agttaatgac cctacaatgc ctaaaggtaa agaaaaagta gacaaaagtg gaagaaatgc
     1261 atattctgta tcaacatata gaacatttaa cgatgcaaat ggtaaaaaaa ttaaaactga
     1321 aaaaatagct aattcatatt atcctaagaa ggaaggtatt atattagttg ggactatgga
     1381 accaaaacca gaggaaaaac caaatataga cgaaaataag aataatcaaa atacaaacaa
     1441 tcaaaataca aataatcaac aaaaaccaga aacaccacca actgataata agcctaatga
     1501 gacacaacca caaccgcaag cttaaagttc tcaagaatag gatttatgta gagtaataaa
     1561 aaatataaat ctaaatagga gggattactt tgaatttaaa taatataacg aattacgttc
     1621 aaagtatttg cgagaatatt tctaatgtct tggatgtaga tgttacttta gttacaaaag
     1681 atttaactag gatagcaggt acaggaatat tcagaggtaa aataggagag acgatatcag
     1741 agaagtcagt gtactctctt gttttaaaag atggaaaatc ctatgttata aataaggaaa
     1801 tagaagagaa ctgtaataaa tgtgcaaata gagaaaattg taaagagtta gctgatatat
     1861 gtactcccgt taaaatggga gagcatatac ttggaatctt gggtatagct gcgtttaatg
     1921 aagagcaaaa agaaaagata ctttcaaaag ataaggagtt aactgctttt gtaaggagta
     1981 tgtctgattt aatatctttt aaactagatg aactttataa acaagaagtt aagactatag
     2041 atgaccttga gagggaagct atagaaaatg ccataaagaa atatggaagc aatacagaag
     2101 gtatgaaaaa agtagctgag gcattaaata taggtatagc tacactctat agaaaagtaa
     2161 aaaaattcaa cattaaaagt taatagtatt gaaaaagagg taaccttttt atgaaaagaa
     2221 gaggattacc ttttttatta tgagaatggt ggaaaaatat gtatagtttt gacttgacat
     2281 attaaatatg ttatcttaaa tatatatttt agagtggagg tgagtattat tttagttaaa
     2341 gatataaaac aagatataga aagcatgcct agaaaaattt tagatatatc tttgatagaa
     2401 gataataaaa gtgatttgac tttttttaat atagaagaag aagatggtga tgaaacatta
     2461 aaagcattaa aacagagtta tgtgactaca aattataaag aagaaactct attaaacttg
     2521 cagggagctt taaattcaaa gtcggataca acgtctaagg agttaaaatt agaatcatat
     2581 aaaagatata acaatgcatt ggatttagca tataaaaatt atataactag tgccattaat
     2641 atggttaata aagctctgga gataaatcct aaagacattg atatactaaa tcttagaggt
     2701 cttttaaagt tattaaaatg tgattttgct aaatcatttg agagttttta tactgcttta
     2761 tgttatgaaa ataatcatat atctagaaaa tatgtaaatc taatttcatc tgaagatttt
     2821 aaaacttttt taggaagata caatcattct ataagattta taaatgaaga attaaattat
     2881 gagtcaatac atattttaga gaacataatt gaagaagagc ctgagctaat agagccatat
     2941 gtaatcttat ctttgttata tgataaacta ggtaatgtaa aaaagagaga aggatattta
     3001 gacaaactta aagagctaga taaagataat cctgtatttg aaaaaaatga agaagaaaaa
     3061 gaagacacaa gcaaaaagga agaaataaaa aaagtaaaaa gaaagaaaag tattttacca
     3121 tatattatag ttggctgtgt tttaataggt atggggatat atttgattca aagcaaaaag
     3181 agaatagaaa acttaaataa ccaaataagt agtaaagagg aaaagttaag tgaaactgat
     3241 aaaaagttag gagaaacgag taaaaaatta gagaaaacga ataaggaatt aaatgaagtt
     3301 aaaaataaag aaccaaaaag agagataact gtagctgatg aggaagattt atattatcaa
     3361 gctttagatt taaagaaaaa caaagaatat gaaaaagcca tagataattt taaaagtgta
     3421 gtttcttctg gtaaaactaa aaaatatatt tctgaatcaa tatatcaatt agcaataaca
     3481 aataaattat taggaaataa ggatgaagct ataaaatatt acaaaaaata tataaatact
     3541 tatactaaaa atgaccagta ttatgatgat tcatattacg agttgggtat gctttattat
     3601 gataatggcg atttaaaaaa tgcacaacag actttttaca gtttgagaag tgaagttcca
     3661 gacagtatgt ataacaattc aaaaataaaa gaaattttaa gtgaaaaata ggtttatata
     3721 gttaggtttt ttgaaacaac caagtatgta ttttattaaa ttaaaataaa aataaattta
     3781 aaaagagatt gtgtcaaatt ataactttct attttaaaga aagatatttg cttgttgata
     3841 aaactacaaa tatatgattt gaagcaatct ctttaatttt tggaggtgga aaatgaaagt
     3901 tttcaatcaa ttagtaataa ttttatctat ctgggtaata ggagagtata taagttcctt
     3961 tatacaagga attatactta ttcctgggag tatagttggt atgctattat tatttctact
     4021 actacaattt aaagttttag atttgagtag tatagaaaat gtaagtggat ttttcctcga
     4081 taatatggct atatttttta ttccagcagg tgtatcactt ataaagtcat tagacctaat
     4141 aagggaaaat gtattagtgc ttttattagt tatatgttta agtacactaa tagtaatgta
     4201 tactactgga attatcgtgg agaaaatgat aaaaaagaag gaagaaggac aaaaaaatgt
     4261 ataatgtatt acagacacca gtatttggta taatagtaac aatagttttt ttcaatttag
     4321 gaagatatat tcaacgaaaa acaagtaatc ctatatgtaa ccctttatta attgcaatcg
     4381 taggaataat attgttttta agcatatcta aaatccctta tgagaattac aagataggag
     4441 ctgatagtct aaatttcttt ttaggtcctg tcacagtagt acttgcagtt ccactttata
     4501 aacaatttga attatttaaa aagcatatgt ttgagattat tgtaggaata ggttgtggta
     4561 tagtgatttc ttttgtatct gtattgataa ttggcaaaat agctaattca gatgtaagta
     4621 taataaattc actaattcca aaatctataa caacaccaat gggaatatct ttagcaaatt
     4681 ctttaaatgg tatagaatca ataacagttg tagctataat ttttacaggt atttttggcg
     4741 gtatggttgc ttctacagta ttcaaacttg ggaatataaa tcatccagtt gccaaaggga
     4801 ttgctcttgg aacatctgca catgctttag gaactacaaa agcttttgaa cttggagaag
     4861 tagaaggagc tatgagtggt gtttctattg gagtttctgg tacaattaca gtaatactaa
     4921 ttccaattat aatgaatttt atataaaaaa taaaattaag acaagtacct tgaatactta
     4981 aaacaaaata aaataaaaaa aatttaaaat aaaaactgaa cgaaaaaaat gcaaataatg
     5041 aaataaaaaa atcatgtact gaaattataa aaagaaaaat ttcatttagg aattaattat
     5101 aaagagataa ttactaaatt tgaatttgct aaaataaaat aaattactgc taaaatggat
     5161 atttagaaaa aaatactata aatttactat attgcaagtt aaataataag cagtatcata
     5221 ttatgctaga aaaagtttca ttcaaagcat aaatgagatg atttgaacgt tttttaagat
     5281 tagaatataa aagattgcac tagacatata tgaaaaagat aatataatag atgatgtaat
     5341 agatgacagt tcaagaaatt atataaaaaa atttatttga aatattatct attacatcag
     5401 gaataatata tgtaagagta agcttttaaa attctaaaga tttagaaaga ttgaagggag
     5461 aaagaaatga gtgaagttaa gaatacccta caaaaacatc cagttttaag atattcaaca
     5521 atgctatttg ggatgttgat gacatctata ggaataaatg ggttcttaag accagcacat
     5581 ctgcttagtg gaggtgctac aggtatagca acatctataa actatttgac aaatataaat
     5641 gtaggtttat taacattttt aatcaatatt ccaatattta tcttagggtt catatacctt
     5701 gaaaaagagt tttgtatttc aagtttggta aatatgattg tattttcatt attattagga
     5761 gcaactcagg aaataagtaa tattatacct atacatgata tattgcttca aagtgtatat
     5821 ggtggtatat tatctggttt aggagttgga gttgtattta gaactaggtc ttctcaaggt
     5881 ggtacagata taatagctgc aatactaaaa attaagaaga atatagaaat gaaagacact
     5941 gctttagcaa taaatggctt gatagtactt acaggaagtt ttttatttgg attggattta
     6001 gcattataca ctcttatagg tctatttcta aatgcgtatt caatgagttt tattaaagat
     6061 gcaatgaatt accaaaaatc tgttatggta atgtcaaatg aagtcgattt aatagcagaa
     6121 gatataatga agagtttagt tagaggtgta acttttttag atgcagaagg tgcatacact
     6181 catcaaaaaa agaagattat atatacaata gtttcatcaa atgagatacc aaagataaaa
     6241 gatatagctt tgaagtatga taaaaaggcc tttatatctg taaatgatgt tacagaagta
     6301 aagggaagag gatttaaagc aaaagattta tagaaaattt tacaagctgg gagtttataa
     6361 tgtaaatttg tatattatat ctctcagctt atttaaattt agtatatgaa aataaaggtt
     6421 ttattaatga tttgtgtatc actttaaaag atatcatttt aaaattaaaa ttgaaaaaat
     6481 tacttaatat gtagaaaaca tgaccactat taggatataa taaatttaat agatttagta
     6541 aaatatctag gaataacgtt ggaggaatgg cattatgaat ttatctaaag aatatgttaa
     6601 tttgtatgat aattatttga aatatataaa tgaagtaaac aaagagatta gaagtataaa
     6661 gaacatgaag aaacgtgttt taaaaagggg cgattttgcc ccagaaaatg aagtattaga
     6721 attggaagga aaaacaatac gcgatataga tgatgtcgat acaaaaaaac caaaaaataa
     6781 aatctataaa ttttcaaatg gggatgtata tataggaaaa ttcttagagg gaaagatgga
     6841 gggaaaaggc tcatatactt tttttgtcga tgaaggaaca gttatggagt acataggaga
     6901 gtttaaagaa gataaaaaaa atggaaaagg aaactttgta ttttcaaatg gaaatgaata
     6961 tgtaggttat tttgaggaag atgtgatgag tggtattgga aatatgttat ataatagcaa
     7021 agatgaatat attggaactt ggaaaaacgg aaaaaaagat ggaaaaggta tttataagtg
     7081 gagtgatgga tgtatatatg caggtgaatt taaaggtgga aagatggaag ggtacggtat
     7141 ttgctataat agtaaagggg aagtacttta tgaaggtgaa tggaagaata atttgattca
     7201 tggtaaggga acttatatat gggaaaaagg taaaaagtat ataggtgaat ttatgcatgg
     7261 taaaaaacat gggcaaggaa ctttttatct aaataatgaa ttggtttatg aaggtacttg
     7321 gaaatttgac aagccatcta tatttgatag aacattagat gaaatatttt ccttgagact
     7381 gtagctttat ctaattagta gataaagctt ttttctaata cttactgtaa ataggtatga
     7441 atgatattga ttagtataca ctatcaatat taacttaata aatgcaatat taaaaaattt
     7501 tgattgcttt taagaaggag agatatattg aagagattaa ttgctttagt gttagtagtg
     7561 cttattgttt tattttatag tcctgaatta ttaaaagaag gatatgattt aaaagaaaag
     7621 ttttttcaga gtgaaatatg gaatgaattt ttagataaaa taaatataaa tgataattct
     7681 aagaaagata aaaaagaaaa aagtcaaaat ttacaatcaa ataatactga aggggagaat
     7741 ttaggagagt cagacataaa gaattttgaa aaaataagct taggagacaa tttaagctat
     7801 gttttatcaa gtattggtaa acctggcaga attgatatta gtgaatacgg ttttgattgg
     7861 tatgtatata atcaatatgg aaaagaattt gcaatggttg gacttgaaaa tgatgaagta
     7921 gtagcacttt atagtaactc tattaattca tgtgaaaatc aagatataaa aataaatcaa
     7981 gatagacaaa cagtgagaac taagattaca ccattaaaat ataagagaaa aggaaatacc
     8041 agatatataa taaattcaga aaatcaatat gatataataa gtaaagaagg taagtatatt
     8101 acaatttttt acgatattca tgaagaaaat agagtttgtt catatttaat aattgataaa
     8161 agtactgaag atgagtttaa aaatttatat ccagatgata gcgaagaact taaaaaatgt
     8221 tttgaacttg aaattataga ccttgtaaat agtgttagaa atcaaagagg gttaaactca
     8281 ttgagataca gtgaacaggc aactttgagt tcaagaaaac atagtgaaga tatgagagat
     8341 aataattttt ttgaccatgt aaataagaag aatgaaactc catttgacag aatgaaaaga
     8401 gaagggatag tatatacttc agctggagaa aacatagcag caggacagat taatgcaata
     8461 tatgcacacg aagcttggat gaactctgaa ggacatagaa aaaatatttt ggggaattat
     8521 aataatattg gagttggagt aatatttgga ggaagctata aaacatatta cactcaaaat
     8581 ttttataaat aactaaaata tttgaaagga agttaaaaaa tgacatcatg gaaaaaaaag
     8641 acagtatata agtgtttaat agcagtagtc ttgttttgtg gaatagtgtt aatatctaat
     8701 tttagcaaag tttctgcttt gatgatggat actaatggaa atgttttgat taaacatggt
     8761 tcaagggaaa aaaagctaat agcaattacc tttgatgatg gtcctcatcc taaagaaact
     8821 agtcaagttt tagatgtgtt aaagaaatac aatgtaaaag ctactttttt tatagctgga
     8881 aaacatgcaa aatggtataa agaaccatta gtaagagcaa gtaaagaagg tcacgagata
     8941 ggaaatcata ctttcaatca tcctgatata agtaatttaa gttctagtca gatagaagag
     9001 gagattgtta agtgtgagga tattttgaag gaagtaactg ggaaaaaacc tacattattt
     9061 agacctccat ttggaagtta tagagaaaaa gacttaatag aaattgcgaa gaagcatgac
     9121 tataaagttg ttttatggac aggtgtagat gtgaaagatt ggaaaaatcc aggagctaat
     9181 agcattgcag ataaaataat taataaagta caaaatggag atataatact tttacatgat
     9241 tatgcaacta atgatacagt tgaagcttta gatatgttta tcccaaaaat gattgaaaaa
     9301 ggttttaaat ttgtaacagt ttctgagttg ataaaataat ttttaggggg agatttacgg
     9361 tatgattctt atgatagaca attatgattc ttttgtatac aacctagtac aatatataga
     9421 agaattaggg gaaacagttg tagtaaaaag aaataatgaa atcaaaataa gtgacattga
     9481 agaactaaat ccagaggtaa ttgtattatc tccaggaccg tgttctccaa aagaagctgg
     9541 tatttgtata gatgttgttg agcattttaa ggggaaaaaa cctatactag gaatttgttt
     9601 aggtcaccaa acgattggac atgtttttgg aggagatatt ataaaagcac aacagcctgt
     9661 gcatggaaaa gtatatagca tcagccatac taataaagga gtttttagtg gacttaaaaa
     9721 tcctttaaat gtcactaggt atcattcact aataattgat tccaatacag ttccaaaaga
     9781 actagaaatt acagcaacta ctgataaagg ggaaataatg gggattaggc ataagaaata
     9841 tttaatagaa ggagtacagt ttcatccaga agctattttg tcagagtatg gtcatgaaat
     9901 gcttaaaaat tttataacag aagctagaga aagggtacat atgtaatatg ataagagaga
     9961 taaacactaa attaaattct tttgagatat ttactatttt tagaaatgaa catgacagtt
    10021 ttatattaga cagtgctatg gataaagaaa aattgggaag atattctttt ataagtagcc
    10081 aaccatttaa ggtattaaag tataaggata ctgatgaaaa tcctttagaa gttttaaaag
    10141 aagaactaca taagtatagg gttgcaaatg atactaatct tccatttgta ggaggtgctg
    10201 tgggatattt atcttatgat ttaggtaatt acatagaaaa tttacctaga actgctgtag
    10261 atgatattga gatgcctgat atgtattttg gattttataa tcatgtaata gttatagacc
    10321 atcttgtgca aaaaacttat atagctactc caaacataga tatagaatta gaagaaaaga
    10381 taatggatga tatagaacaa agaatattaa aagaagagaa aaaaggtata gatagcatat
    10441 gttatgaaga aaaagaagta acacctataa gacttaaatc taattttaca aaggaagaat
    10501 ttaaaaatgc agttcaaagt gttagagagt acataagaca aggagatata tatcaagcta
    10561 atttaacaca aaggttcagt ggagaaacag aacttacaag ctttgaattg tatagggact
    10621 taagaagatt tagcccagca ccatttgggg cattcttaaa ctttgaagat acacatatat
    10681 tatcaaattc tccagaaaga tttataaaat gtgttaataa gagaatagaa actagaccaa
    10741 taaaaggtac tcgtccaaga ggtaaagata aagaagaaga tttaaaactt caacaagaac
    10801 ttagaaatag tgaaaaagat agagctgaac tacttatgat agtagattta gaaagaaatg
    10861 atattggcag aatatctaaa actggaagtg ttaaagttcc agaactattt gtaattgaac
    10921 catatgcaaa tgtaaatcac ttagtttcca cagttgtagg tgagttaaaa gatgataaag
    10981 atgctactga tgtaataaaa gcaacttttc caggggggtc tataacagga gcacctaaga
    11041 ttagagcaat ggaaattata gacgaattag agccaacgca aagaaatgta tatactggtt
    11101 caataggtta tataggtttt aatggtgata tggattttaa tatagcaatt agaacaataa
    11161 tcaaaaatga taaaaaagtc tattttcaag ttggaggagg aatgacttgg gattctgacc
    11221 cagatgaaga atatcaagaa acacttgata aagcaaaatc tataatgaag gctttgaggg
    11281 gatattatga agaataatgt aggttttgat agtagtttaa gtaaatttgg aattggtttg
    11341 tttgaaacta ttagagttaa aaatggaata gcaatagatt taaatatcca tatagaaaga
    11401 atgataagtt caataaattg tttggattta gatataaact ataaaaaaga tttcttgata
    11461 aatgaaattg ttacatatat aaaaaaagaa aatgtcatta ataaagcact tagaattact
    11521 gtttttgatg aaggttataa tatatctatt agggatattc catataatca agagacgtat
    11581 gagaaggggt ttaaattagc tatatcgcca atagttaggg ggaatagttt aattcataga
    11641 cataagacta ctaattattt tgaaaacata tataccaaaa atatcgcaaa taagactggt
    11701 tataatgatg gtatattttt aaattcagaa ggtgtaatat tagaatgttc tatgagcaat
    11761 atcttcttta taaaaggaga aagagtgtgc actcctagtg atagattacc gatattaaat
    11821 ggaataataa aaaaaaggat tatagagatt tgtgacgaat tacatataga gttgatagaa
    11881 aatgaaatta atatatcaga aattagtagt tttgattttg tttttgtcac aaatagtctt
    11941 atgggcgcta taaaggttac agaaattgac aaaataaaat ttaataaaga aaatgttgtt
    12001 tttgataaaa tagtgaagtt attagaataa attaaaatat taacaatttg ttggagacaa
    12061 aatatgatag gttgtattta tgaagtatac aatgaagaga tgactaaaat aatagaaaac
    12121 caaaacactt tggctgtata tttggtgggt tctagtaaag atgtagattt tactctttat
    12181 gatgtagaga taaacgatat tgatgtgttc gtttttgtaa aagaagggga aaaacaagag
    12241 agaatactgt ttaaaaaaaa gggaatagaa tttgatataa attacttttc aaaagatggt
    12301 gtaaaaaaat tattatcaaa tagggaatat ttctttgtga aagaaatgaa agatgctaaa
    12361 gtactgtttg atagggagag ta
//
LOCUS       DAEMAE010000108        12180 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEMAE010000108 DAEMAE010000000
VERSION     DAEMAE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.110"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            511..675
                     /locus_tag="KPH93_002293"
     CDS             511..675
                     /locus_tag="KPH93_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689103.1"
                     /translation="MENKKLQIEKITDKIEIEEVEVQADPCKNDPYNGIYDCLRDCSS
                     GTSPKGSPAY"
     gene            1084..3627
                     /locus_tag="KPH93_002294"
     CDS             1084..3627
                     /locus_tag="KPH93_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1689104.1"
                     /translation="MKKQRGKTVALLSSIILSVMLIFSIIVIRASGYDSQIQEAKNLH
                     GEYQIWFEGIDINKAKKLKNDTEVKKSSSVEYFCEVINKASGVKLALNSFDKDFIESL
                     NYKMIGRNPTKEGEIVIEKEVAKQIGISNPLNKNIDLMLMNKYVDSDGTNKMDSANQT
                     FKIVGLIEKPNKYYTSLSGSLGGIKTQAFIYKGTKLPIRTEDTYKGVIFLKSEKNTSK
                     FISKMEKKLNLTWDYLHENTEVSAAKLLKDLSENKENIQKMFLLVIVSSLVIYNIFNI
                     ILQDMIVQIGLMRSIGMSNKKIKSMFAVLSLIYIVLGTIIGMLLGMILSYIGVRLIYG
                     YSSMMTVEISSIIYSFSVSILAVSISSFIVVRKAVKMSIINSIRSSEMYERKLKYSSN
                     KTNKTQGNIIRSLAIRNIGRNKSRTIITILAITMVGTMFIFNLGTKSLLKANIESGVT
                     GGGLGMSYGSVDKTVSGSYNGTESLFYKIDESMIEEIKNIEGVESVEPNFFNPNGYIL
                     LSKDKISKSYKEELDRRNLLFQDKNDNEYPLLIRGYSDELLKKRESFIEEGKNLINTK
                     NGQYKKVMLVNNTNSQVTHSFDAKVIENVKVGDVIDIKLPVYKDGTQKYETFKVEVSA
                     IMKEVYAAAQDGNVHASGAQVIFREDDYRELTGQKEYNKLYVMAQKGKLYPVEKKLEE
                     LTKNYSFTSIGGKGEDLKLVGVQQSSEERLAVIYQCLIILILAVNSIFIMRSNIISRR
                     KELSTLRAIGMSIKSIKKVLIIESELYGIIATIIGTIIATIYYNWGISKSNKTLLAGG
                     FNRTIEYNIPFNQIFILFAIFIIMGFVAVYLSKDKIEGTEITEGISQND"
     gene            3662..4939
                     /locus_tag="KPH93_002295"
     CDS             3662..4939
                     /locus_tag="KPH93_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1689105.1"
                     /translation="MFDDIQIKFRENDLLINKRNGKVILYNKEKKRHVKLSEEVYEYT
                     RLAEKNNWKVRELIDCFKKDEDKKYIESILNIMVKNEVIDGNLFNKKNFKDMHLSLTN
                     RCNLSCNHCVSSCSPKEEDYMDTSMILKLIDNLCELNVRSLVLTGGEPLVREDFFEIV
                     NYIKKKLPLEELSLSTNATLINDTNIDFIISKFHKVDISLDGIDEETCSNIRGKGVFK
                     KVLNVVYELQNKKFNNISLSMVFGEKNYQMSEKFKKLNEDLNTKAIERYFLPSGRGLI
                     NRKIYSDENSVLPLVIPKFRENEINGKKNRINPCSCSCNACIEQVFIDHKGNIYPCPS
                     LVEKEYLVGSVFDKKIINNLKENELKENQAFRNLNEIYPFNFEKCKDCDVNIFCFKCP
                     AMVNKVKNDDKELNIWCNLTKRNMELAVWGGEV"
     gene            4940..5986
                     /locus_tag="KPH93_002296"
     CDS             4940..5986
                     /locus_tag="KPH93_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1689106.1"
                     /translation="MKFTIFVTEKCNLRCTYCYERGMDRGKSMDINTANKTIDFICKN
                     IKNEELDIPLYIVFHGGEPLINFKLVKHIHKELSKRVLERKIIYDMTTNATYLDEEII
                     NFMCKNIDNISISIDGTKKFHDKNRIFSNGDGSYDLVIKNAKRFLDIGKDVRVRMTYT
                     TDTISELYESIAQIVRYGFTNIVPIADFMSLNWTNNHIDIINEQISKLINLKKEHPNI
                     DISLLDIKNLNIKKGDCFGGIVSFAIDVNGDIFPCTFSVGDNEFKLGSVFDRNISEEK
                     RQELIKIYISENEECKDCLRKEWCTSVRCKLMNRKVTGEFNKAIPIICALEKVILTTT
                     RKIKMEEKSKEAMI"
     gene            6050..7288
                     /locus_tag="KPH93_002297"
     CDS             6050..7288
                     /locus_tag="KPH93_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1689107.1"
                     /translation="MEDRFFINDVCDNIINENQVILANKINGKWIKIPIECYKVIEYS
                     IKNKKPLDKLEDVFNLDEDKKYYKQVVDKLEQLGFVSNFIVDKFKLERVNLVSFSPTN
                     RCNLRCDYCCADSKIENKDYLGTEELKEAIDNIVKINPRILTITGGEPLLRDDFFEIL
                     SYTKEKFKGKIRLSTNATLIKENQVDEIIDGLYAMEISLDGYDELSCSKVRGKGVFTK
                     VINLIKYIKSKGFNNIGLSMVVGKNNENDIDKFNKINYELGTIPIIRNFMNIGRGNES
                     YTRYLDDELDIGYICEDDYINKNSLNSNMCKAGVNQISIDYKGDVYPCPNLEYDDLKM
                     FNILQFDESMLEKILNRNMEIFDKFDILKPMNMEECKDCKVNVFCTTCPSKMYTLKNN
                     KEMLNSNCKKMKSILEPIVW"
     gene            7369..8412
                     /locus_tag="KPH93_002298"
     CDS             7369..8412
                     /locus_tag="KPH93_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG1689108.1"
                     /translation="MEKIKVAVVDTGIDVNDDSIKKYIKFNKNIQLEEVVEYKDLDDT
                     HGHGTLCSKTILSVCRNTDRIEIYPIKIFNQGGCTSGERLLEALERNLDSDIDLINIS
                     ASTLNDKYKRELKYICEELCKKGKIIISSHHKRAKDCDSFPTVFNSVIGVTGSNDIYN
                     DNDYIYEKGKKIQIIANTKECFVEFRNEVTHFGKSSRASAVITGTICNIFNKYGKIMF
                     DELEKIFEKESLDKNINTVKSKFRNKQNNFDLCITKNQKLDISRRIVNMINKKFAVHK
                     IDLDFLDKYSIFNNFTDLGNHNAFKFLTEINKEFEIEISYKGLFLYELENLNDLVNLV
                     CKHLNKASVNNSI"
     gene            8510..9229
                     /locus_tag="KPH93_002299"
     CDS             8510..9229
                     /locus_tag="KPH93_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689109.1"
                     /translation="MKTLKIKNLKKIYGKYENEVHAIAGIDLEIEPKKFTAIVGASGS
                     GKSTLLHCMAGLDKPTSGNVYLDDLDIYTLNDDKLSKIRREEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDSKREDKTHINNLIFRLGLESQVKRFPNELSGGQQQRVAIARALANKP
                     SVIFADEPTGNLDSKTTAEVMELLEICVKEFSQTLVMITHSDKIAETADKIITISDGK
                     IVKILDKSFEEHNMGETEIKI"
     gene            9354..11207
                     /locus_tag="KPH93_002300"
     CDS             9354..11207
                     /locus_tag="KPH93_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689110.1"
                     /translation="MDEMKKKCSKNKKIENRNNKNKSLKRFAFLTFKKNKGKLLLSFI
                     MVIIVAVVDLVQPQVTRMIVDEGIGTKDANLLLKLMFIYGAAGVSSAILYLLLSYLYS
                     AIKKNVTVDIKIKLLNHLSKLSGKYYSNIKTGNIISIIESDIFIIENFGAELAFSILV
                     NIFTASMALFFLVRMQVDLFLIVLILQIILSIFQNKFTKLISKNTREIREDSGSLSNK
                     IQEYVSNIKNVVVSKSRMKFFSSYLSEEKNLIRKYLKLDLIMNGNIAIAKSISILVTV
                     SIYGVGGYKIISGNMTIGELLAFQQYTNMFIGPCISIVRANTRIQQAKASLDRVYSVI
                     DEEIDIKVNNRGLRIEDKECNIIEFKNIYFSYKEIGKKKLQNLEQDEQIDCTYEDIET
                     DSYILSNINITFRKGETTALIGESGSGKSTIVNLLYRLWDIQKGEITIDGINIKNINL
                     KSLRKSINIVTQDMLMFDDTIRNNININKSLSDEELKNICSVVGMNDFISRLENGFDT
                     IIGEKGVKISGGQKQRIALARSLVNDSKILILDEATSALDNISQSEILRNIRAYVKGK
                     IVIIIAHRLSTIKDADNIYVLEKGKVVESGKDKELFIRNGIYRKLVTLNTQ"
     gene            complement(11974..>12180)
                     /locus_tag="KPH93_002301"
     CDS             complement(11974..>12180)
                     /locus_tag="KPH93_002301"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1689111.1"
                     /translation="MSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKKMISLINDYI
                     YFYNNERIQLKNGMSPIEYRTHCA"
BASE COUNT         5122 a         1119 c         2033 g         3906 t
ORIGIN      
        1 ctataacata tttaaaaggc agtaagaaga catatattta aaaaataaat taatcttaat
       61 atacagtaaa aaagattata aaaatataaa tcaaagtctg gaagtatatg agtgttttat
      121 tgaaactaag aagaaaaatt taacaaagat gtatttggta attatcaggt gtatataaga
      181 cagttaagaa ataaaataag taaatttctt ataaaaagaa aattaagaaa tattaacata
      241 atatcgttta tttatttttc tattaaaaag gttttataga tttatttttt atattaagat
      301 aagtaattta taggagatac aaataattaa atctgtaatt acagagcaat tgttagaatt
      361 gtaatctgtt ttacatatag aaaatattta tttatatatt ttatcgatag gataaggcgc
      421 cgtgtataaa aaatattttc aaacaaatta aattgatatg tagcttgtgt aagttaaaaa
      481 gtacaaagtt acagaaaggt aggaataatt atggaaaata aaaaattaca aatcgaaaaa
      541 atcactgaca aaatagagat agaagaggta gaggtgcaag cagatccatg taaaaatgat
      601 ccatataacg gaatatatga ttgcttaaga gattgtagct caggaacatc accaaaaggc
      661 tcaccagcat attaagtttt actatgaaag tttagattat tgacaacaat aggccacctg
      721 ctatgcgggt ggcattcttg gttatacgaa aattaatcat attctaatat aaactattta
      781 aggatttaac ttaataaagt tatttaaaaa ttatgcaact ataaaggtat agatataata
      841 gaataacata taaatttaga ttatacatgt tagccaaagt ctcaatttaa aattttatgt
      901 agtacttgaa ctgcaccaaa cttaaagtgt aaataggata atagaaagtt ttataaataa
      961 gaatattatg taattaagca atgagttatg tccccaaaat agaaaatttg ttttataagg
     1021 gatgataaat ttttgtcatg gaggtgagaa gttttgttta tagtaaaact tgcaatttct
     1081 tatatgaaaa aacaaagagg aaaaacagta gcactattat cgagtattat actttcagtc
     1141 atgcttattt tctcaattat tgtaattaga gcctcgggtt atgattctca aatacaagaa
     1201 gcaaagaact tacatggtga atatcaaata tggtttgaag gaattgatat aaataaagca
     1261 aaaaagttga aaaatgatac tgaagtaaaa aaatcaagca gtgttgaata tttttgtgaa
     1321 gtaattaata aggcaagtgg tgttaaactt gctttaaatt catttgataa agattttata
     1381 gagtcactta attataagat gataggaaga aatccaacta aagaaggcga aatagtcata
     1441 gaaaaagagg ttgcaaaaca gataggtata agtaatccat taaacaagaa tattgattta
     1501 atgttaatga ataagtatgt tgatagcgat ggaactaaca aaatggatag tgccaatcaa
     1561 acttttaaaa tagttggttt aattgaaaag ccaaataaat actatacctc tctatccggt
     1621 tcactaggag gaattaaaac tcaggctttt atatataaag gcacaaaact tccaataaga
     1681 actgaagata catataaagg tgtaatattc cttaaatctg aaaaaaatac atctaaattt
     1741 atcagtaaaa tggaaaagaa actaaatctt acttgggatt acttacatga aaatacagaa
     1801 gttagtgcag caaaactatt aaaagattta tctgaaaata aggaaaatat tcaaaagatg
     1861 tttcttttag ttattgtatc tagtcttgta atctacaata tttttaatat tattctgcaa
     1921 gatatgatag ttcaaatagg acttatgaga tcaattggta tgtcaaataa gaaaatcaag
     1981 agtatgtttg cagtacttag tcttatttat atagttttag gaacgataat aggtatgtta
     2041 cttggaatga ttttatcata cattggagtg agattaatct atggatatag ttctatgatg
     2101 actgtagaga tttcaagtat aatttattct ttttcagttt ctatattagc agtgtcaata
     2161 agtagtttta tagtagtcag aaaagctgtg aagatgtcta taattaattc aataagaagt
     2221 agtgaaatgt atgaaagaaa attaaaatat agtagtaata aaacaaacaa aacacagggt
     2281 aacataatta gaagtcttgc aattagaaat atagggagaa ataaatctag aacaattata
     2341 acaatacttg cgattaccat ggttggaaca atgtttatat ttaaccttgg cacaaaaagt
     2401 ctattaaaag ccaatataga atcaggagta actggtggtg gacttggcat gtcatatgga
     2461 agtgtagata aaaccgtaag cggtagttac aatggtacag aaagcttatt ttacaaaatt
     2521 gatgaaagta tgatagaaga gataaaaaat atagaagggg tagaaagtgt agaaccaaac
     2581 ttttttaatc ctaatggata tattttacta tctaaggata aaatatcaaa atcttataaa
     2641 gaggaactag atagaagaaa tttactattt caagacaaaa atgataatga atatccactt
     2701 cttataagag gttatagcga cgaattatta aagaaaagag aatcatttat tgaagaaggg
     2761 aaaaatttga taaatactaa aaatggacaa tataagaaag ttatgttggt gaataataca
     2821 aattcacaag ttacacattc atttgatgct aaggtaattg agaatgtaaa ggttggagat
     2881 gttatagaca taaaattacc tgtatacaaa gatggtactc aaaagtatga aacatttaag
     2941 gttgaagtta gtgcgattat gaaagaagta tatgctgcag ctcaagatgg caatgttcat
     3001 gctagtggag cacaagtaat atttagagaa gatgactata gagaactaac aggtcaaaag
     3061 gaatataata agctttatgt gatggctcaa aaaggtaaat tatatccagt ggaaaaaaaa
     3121 ttagaagaat taacaaagaa ttattcattt acatcaattg gtggaaaagg tgaggatttg
     3181 aaactggttg gagtacaaca aagctcagag gaaaggcttg cagtaatata tcaatgccta
     3241 attatactta tacttgctgt aaatagtatt ttcattatga gaagtaacat tatatcaagg
     3301 agaaaagaat tgtcaacgct aagagcaatt ggaatgagta tcaagagtat taaaaaagtt
     3361 ctaataattg aaagtgaatt atatggaatc attgcaacta taataggaac tataattgca
     3421 accatatatt ataactgggg aatttcaaaa tcaaataaaa ctcttttagc aggaggattt
     3481 aataggacta tagaatataa cattcctttt aatcagatat ttatactatt tgcgattttt
     3541 attattatgg ggtttgttgc agtatatcta tctaaagata agattgaagg aacagagata
     3601 acagagggta tttcacaaaa tgattaagtt tgagattcta agataacatg gggtgataag
     3661 attgtttgat gatatacaga taaagtttag agaaaatgat ttattaataa acaaaagaaa
     3721 tggaaaagtt attctatata ataaagaaaa aaagagacat gtaaaattat cagaagaagt
     3781 atatgaatat actagacttg ctgaaaaaaa caactggaaa gtcagagaat taatagattg
     3841 ttttaaaaaa gatgaagata agaaatatat cgaatctata ttaaatatta tggtaaaaaa
     3901 cgaagtaatt gatgggaatc tattcaacaa aaaaaacttt aaagacatgc atctttctct
     3961 tacaaataga tgtaatctta gttgtaatca ctgcgtttca agttgtagtc ctaaagaaga
     4021 agattatatg gatactagta tgattttaaa attaatagat aatttatgtg agttaaatgt
     4081 aagaagtttg gttttaactg gtggagagcc attggtcaga gaagattttt ttgaaattgt
     4141 gaattatatc aaaaaaaaac ttccattaga ggaacttagt ttatcaacaa atgcaacatt
     4201 aattaatgat acaaatattg actttattat cagtaaattt cataaagttg atataagttt
     4261 agatggaatt gatgaagaaa catgtagcaa cataagagga aaaggtgtat ttaaaaaagt
     4321 attaaatgta gtgtatgaac ttcaaaataa gaaatttaat aatataagtt tatctatggt
     4381 ttttggtgaa aaaaattatc aaatgtcaga aaaatttaaa aaacttaatg aagatcttaa
     4441 tacaaaagct attgaaagat attttcttcc gagtggaaga ggtctaataa atagaaaaat
     4501 ttactcagat gaaaactctg ttttaccact tgtgatacct aagtttcgtg aaaatgaaat
     4561 taatggtaag aagaatagga taaatccgtg ttcgtgttca tgtaatgctt gtattgagca
     4621 agtatttata gatcataagg gaaatatata tccttgtccc tctcttgtgg aaaaggaata
     4681 cttagttgga agcgtatttg ataaaaaaat tataaataac ctgaaagaga atgagctaaa
     4741 agaaaatcaa gcttttagga atcttaatga aatatatcca tttaactttg aaaaatgtaa
     4801 agattgtgat gtgaatattt tttgttttaa gtgtccagct atggtaaaca aagtaaaaaa
     4861 tgatgataaa gaattaaaca tatggtgtaa tttaacgaag agaaatatgg agctagcagt
     4921 ttggggaggt gaagtataaa tgaaatttac gatatttgtt acagaaaaat gtaatttaag
     4981 atgtacatat tgctatgaac gaggaatgga tagaggtaag agcatggata taaatactgc
     5041 taataaaacc atagacttta tttgtaagaa tattaaaaat gaagaacttg atattccatt
     5101 atatattgta tttcatggtg gagaacctct tataaacttt aaattagtaa agcatataca
     5161 taaagagcta tccaaaagag tattagaaag aaaaattatt tatgatatga cgactaatgc
     5221 aacttatttg gatgaagaaa ttataaattt tatgtgtaaa aatatagata atatttcaat
     5281 tagtattgat ggaactaaga aatttcatga taaaaataga atattttcaa atggtgatgg
     5341 tagttacgat ttagtgataa aaaatgctaa aagattttta gatataggca aagatgtcag
     5401 agtaagaatg acatatacaa cagatacaat ttctgaacta tatgaaagta tagctcaaat
     5461 tgtaaggtat ggatttacta atatagtacc aattgctgat tttatgagtt taaactggac
     5521 aaataatcat atagatataa ttaatgagca aatttcaaag ttaataaatt taaaaaaaga
     5581 acatccgaac atagatatta gcttacttga tattaaaaat ttgaatatta aaaagggaga
     5641 ctgttttggt ggaattgtca gttttgcaat agatgttaat ggagatatat tcccatgtac
     5701 ttttagtgtt ggagataatg agtttaaact tggaagtgtc tttgacagga atattagtga
     5761 agaaaaaaga caagagctta ttaaaattta tatttcagag aatgaagagt gtaaggattg
     5821 cttaaggaaa gagtggtgta cttcagttag atgtaaactt atgaatagaa aagttacagg
     5881 ggaatttaat aaagcaattc caataatatg tgcactggag aaagttatct taacaacaac
     5941 aagaaaaata aagatggaag agaaatctaa agaggcaatg atatagcata aaaatttaat
     6001 gtggaagtac aattctttat actgtcataa atataggggg taataaaata tggaagatag
     6061 attttttata aatgatgtat gtgacaacat aataaatgaa aatcaagtaa ttcttgctaa
     6121 taagataaat ggtaaatgga taaaaattcc tatcgaatgt tataaagtta tagaatattc
     6181 aataaaaaat aaaaaacctc ttgataaact ggaagatgta tttaatcttg atgaagataa
     6241 aaaatattat aaacaagttg tagataaatt agaacaatta ggttttgtaa gtaattttat
     6301 tgtagataaa tttaaattag agagagtgaa tttagtttca ttttctccaa caaatagatg
     6361 taatttaaga tgcgattatt gttgtgcaga ttctaaaatt gaaaataagg attatttggg
     6421 tacagaggag ttaaaagaag caatagataa tatagttaaa ataaacccaa gaattttaac
     6481 tattacaggt ggagaaccat tacttaggga tgattttttt gaaatactat cttatacaaa
     6541 agaaaaattc aaaggaaaga taaggttatc tacaaatgcg actttaatca aagagaatca
     6601 ggtagatgag ataattgatg gtttatatgc aatggaaatt agcttagatg gttatgatga
     6661 attaagctgt agtaaggtaa gaggaaaagg tgtatttact aaagtaataa atttaattaa
     6721 atatattaaa tcaaaaggat ttaataatat tggtttgtct atggttgtag gaaaaaacaa
     6781 tgaaaatgac atagataaat ttaataagat aaattatgaa ttagggacaa ttcctataat
     6841 aagaaatttt atgaatattg gtagaggaaa tgaaagttat actagatatc ttgatgatga
     6901 acttgatatt ggatatattt gtgaagatga ttatataaat aaaaatagtt taaattcaaa
     6961 tatgtgtaaa gctggtgtaa accaaataag tattgattat aagggggatg tatatccatg
     7021 tccaaactta gagtatgatg atttaaagat gtttaatata ttacaatttg atgaaagtat
     7081 gttagaaaaa attttaaaca gaaatatgga aatatttgat aagtttgata ttttaaagcc
     7141 tatgaacatg gaagaatgca aagattgtaa agtaaatgta ttttgtacaa catgcccatc
     7201 taaaatgtat actttaaaaa ataataaaga gatgttaaat tcaaattgta aaaaaatgaa
     7261 aagtatttta gagcctattg tgtggtaaat actattattt taaatatata catactaaaa
     7321 atatttttat aatataaaga tttaagaaag gtaaaagttg gttgttggat ggaaaaaata
     7381 aaagtagcag tagttgatac tggcatagat gtaaatgatg attctataaa aaaatatata
     7441 aaatttaata aaaatattca gcttgaagaa gtagttgaat ataaagattt agatgataca
     7501 catgggcatg gaactctttg ttcaaaaaca attctatcag tatgtagaaa tactgatagg
     7561 attgaaattt atccaataaa aatttttaat caaggagggt gtactagtgg agagagatta
     7621 ttagaagcat tagaaagaaa tttagattca gatatcgatt taataaatat ttcagcatct
     7681 actttaaatg ataaatataa gcgagaatta aaatatatat gtgaagaact ttgtaagaaa
     7741 ggaaaaatta taatatcatc tcaccataaa agagctaagg attgtgatag ttttcctact
     7801 gtttttaatt ctgttatagg tgtaactgga agtaatgata tttataatga taatgattat
     7861 atctatgaaa agggaaaaaa gattcaaatt atagcgaata caaaagagtg ttttgttgaa
     7921 tttaggaatg aagtaactca ttttggaaag agcagtagag cttcagctgt tataacaggg
     7981 actatctgta acatatttaa taaatatgga aaaataatgt ttgatgaact tgaaaaaatt
     8041 tttgaaaaag aaagcttaga taaaaatatt aatacagtta aatcaaaatt tagaaataag
     8101 cagaataatt ttgatttatg tattaccaaa aaccaaaaat tagatatttc aagacgaatt
     8161 gtaaatatga taaataaaaa gtttgctgta cataaaatag acttagattt tttagataaa
     8221 tattctatat ttaataactt tacagattta ggaaatcata atgcgtttaa gtttcttaca
     8281 gagataaata aagaatttga aattgagata agttataaag gattgttttt atatgagcta
     8341 gagaatttaa atgatttggt gaatttggta tgtaaacatt tgaataaagc tagtgtcaat
     8401 aactctattt aactaaaatc tattttacat aaacgaaaat acaataaaat tgaatatttt
     8461 atttgatatt ttaaaattgc acattactag aaaagaaatg gaggaaataa tgaaaacact
     8521 aaaaataaaa aatttaaaaa agatatatgg taaatatgaa aatgaagttc atgcaatagc
     8581 aggaattgac cttgaaatag aacctaaaaa gtttacagca atagtagggg ctagtggttc
     8641 tgggaaaagt acattacttc attgtatggc aggtcttgac aaaccaacat caggaaacgt
     8701 gtatttagat gatttagata tttatacatt aaatgatgat aaattatcaa aaatacgtag
     8761 agaagagttt ggatttatat ttcaaagtta taatcttata cctgttataa atgtatatga
     8821 caatatagta ttacctgtat tattagattc aaaacgagag gataaaacac atataaataa
     8881 tcttatattc cgtttaggat tagagtcaca agtaaaaagg tttcctaatg agctttctgg
     8941 aggtcaacaa caaagagttg caattgcaag ggccttagca aataaaccat ctgtgatatt
     9001 tgcagatgaa ccaacaggaa acttagatag taagactaca gcggaagtaa tggagttgct
     9061 tgaaatttgt gtaaaggagt tttctcaaac acttgttatg attactcata gtgataaaat
     9121 agcagagact gcagataaaa taattactat aagtgatggg aaaattgtga aaattttaga
     9181 taaaagtttt gaagagcata atatgggtga aactgaaatt aaaatttaat gttgttaaag
     9241 aagaataact tttgaataat aaaatccata tagtaaagac atatcaataa tatagaatat
     9301 taaaataaaa agtcttggaa gttatattta gtattgatta ataattaaat aaggtggatg
     9361 aaatgaagaa aaagtgttct aaaaataaga aaatagaaaa taggaataat aagaataaga
     9421 gcttaaaacg atttgcattc cttactttta agaagaataa gggaaagctt ttactttcgt
     9481 ttattatggt aataattgta gcagtagttg atttagtaca gcctcaagta acaagaatga
     9541 tagttgatga agggatagga acaaaagatg ctaaccttct tttaaaacta atgtttatat
     9601 atggagctgc tggagtatct tcagcaatac tttatcttct tttatcatat ttatattcgg
     9661 ctataaaaaa gaatgtaaca gtagatataa agataaaatt actcaatcat ttatctaaac
     9721 tatcaggaaa atactattca aatataaaga cagggaatat tatttcaata attgaaagtg
     9781 atatatttat aatagaaaat tttggagcag aacttgcatt ttctatatta gtaaatatat
     9841 ttacagcttc aatggcttta tttttcctag taagaatgca agttgatttg tttttgatag
     9901 ttctaatact tcaaataatt ttaagtattt ttcaaaataa atttaccaaa cttatatcta
     9961 aaaacacaag agagataagg gaagattcag gaagtttatc aaataaaatc caagaatatg
    10021 tttcaaatat caagaatgta gttgtttcaa aatcaagaat gaagtttttt tcatcatatc
    10081 taagtgaaga aaaaaatctt ataagaaagt acttaaaact ggatttaata atgaatggga
    10141 atattgcaat cgcaaaatct attagtatct tagtaacagt atccatttat ggtgttggag
    10201 ggtacaagat aataagtgga aatatgacaa taggagaact gcttgcattt caacaatata
    10261 caaatatgtt tattggacca tgtataagta tagtgagagc aaatacaaga atacaacagg
    10321 ctaaagcatc tttagataga gtatactcag taatagatga ggaaatagat ataaaagtaa
    10381 ataatagagg attgagaata gaggataaag agtgcaatat aatagaattt aagaatatct
    10441 atttttcata taaagaaata ggaaagaaaa aattacaaaa tttagaacaa gatgagcaaa
    10501 ttgattgtac atatgaagat atagaaacag atagttatat tctaagtaac ataaatatta
    10561 catttagaaa gggagaaact acagctctta ttggtgaaag tggaagtggg aaatctacaa
    10621 tagtaaattt actttacaga ttatgggata ttcaaaaagg tgaaataact attgatggaa
    10681 taaatataaa aaatataaat ctaaaatctc ttagaaaaag tataaatatt gttactcaag
    10741 atatgcttat gtttgacgat acaattagaa ataatataaa tataaataaa agtttaagtg
    10801 atgaagaact taaaaatata tgtagtgtag taggaatgaa cgattttata agtagacttg
    10861 aaaatggatt tgatacaatt ataggagaaa aaggagtcaa gatatcaggt ggtcaaaaac
    10921 agagaattgc actcgcaagg tcattagtaa atgatagtaa gatattgata ctagatgaag
    10981 caacatctgc acttgacaat atatcccaaa gcgagatatt aagaaatatt agggcgtatg
    11041 taaaaggtaa gatagtgatt attatagcac atagactttc aactataaaa gatgcagaca
    11101 atatttatgt tcttgaaaaa ggaaaagttg tggaatcagg taaagataaa gaactattta
    11161 taagaaatgg tatatataga aaactagtta ctttaaatac acaatagaat tattacttat
    11221 agtagaaata gatataattc aataacttta atggaatata cagaatatta tttaaaatat
    11281 tgttatgaaa aaaagtaatc attttattta ttttatcttt gttaaagtat tttaaatttt
    11341 cgtttgagac aagtaagaat ttttctaagg tttcacggta tagcattttc tttatgttat
    11401 accgtttttt cttaaagaag aaagagtaga caatctattg aaatcacaac aaggcatacc
    11461 tatccaaagc gtaataagtt atgtccacac cattcatctg ctaaaactaa gcattcaaaa
    11521 aactgatgac aaatcctata aacacttcct attctcactt caaaattaaa atttatcaga
    11581 taatttcttg tataaattat actatggctt tatattctag ttgctttttt ttatttgata
    11641 aatatactta ttgtttaata atctctctag aagaaaataa tgaagaaaca aaaatatcac
    11701 gtgacgacag tttagccaca aattactttc tagattgtct aaattaatag gggctgaagt
    11761 tatgagtaaa aaaataaacg tataacatag actataaatg tgcgacataa agcatggata
    11821 tatgatataa actttgaata gtatattata atataggtca aagtatcaaa tgtagaagga
    11881 ttatatttat tacaaactaa tattgacaaa taaaaggctt gctcttatat ggatatgtcc
    11941 ctgtcaagta gacagactta aaaaaggcta tctttacgca caatgagttc gatattcaat
    12001 cggactcatt ccatttttta attgtattct ttcattatta taaaaataaa tataatcatt
    12061 tattagacta atcattttct ttggatctgt agtatcaatt aaatatatta attcagattt
    12121 aaaatgactg aaaaaacttt ctataggagc attatcccaa cagttgcccc ttcgggacat
//
LOCUS       DAEMAE010000109        12171 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEMAE010000109 DAEMAE010000000
VERSION     DAEMAE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.111"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(154..411)
                     /locus_tag="KPH93_002302"
     CDS             complement(154..411)
                     /locus_tag="KPH93_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689112.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKGDYEQAEALINSKLR"
     gene            737..1249
                     /locus_tag="KPH93_002303"
     CDS             737..1249
                     /locus_tag="KPH93_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689113.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSVYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKIPSDKELKSIVFTIQNEGNAIK
                     LYDTQDETYSGCKIIVGMEFETGCVFVDGSDELNDEITAFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            1485..2189
                     /locus_tag="KPH93_002304"
     CDS             1485..2189
                     /locus_tag="KPH93_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG1689114.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDSNEN
                     LEEFFTDVILKGGQNR"
     gene            2186..2956
                     /locus_tag="KPH93_002305"
     CDS             2186..2956
                     /locus_tag="KPH93_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG1689115.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTVLEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            2953..3732
                     /locus_tag="KPH93_002306"
     CDS             2953..3732
                     /locus_tag="KPH93_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG1689116.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGVTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEV
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            3784..4476
                     /locus_tag="KPH93_002307"
     CDS             3784..4476
                     /locus_tag="KPH93_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689117.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKINGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            4495..5919
                     /locus_tag="KPH93_002308"
     CDS             4495..5919
                     /locus_tag="KPH93_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG1689118.1"
                     /translation="MKNQSLIAQFRRTFIFIIIASIVATVITYVFAIYLYIYSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKTFKTKEELFNNFINKTVRKGGYIYTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASNKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNRISYEICDSGSGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(6159..8627)
                     /locus_tag="KPH93_002309"
     CDS             complement(6159..8627)
                     /locus_tag="KPH93_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689119.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIFLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKPILDKIDNM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSTLEDFK
                     NGSICFIKNPIAISTPGVKTKYTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWVNG
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSDLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(8630..9298)
                     /locus_tag="KPH93_002310"
     CDS             complement(8630..9298)
                     /locus_tag="KPH93_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689120.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSIIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHSEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPNKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(9583..10110)
                     /locus_tag="KPH93_002311"
     CDS             complement(9583..10110)
                     /locus_tag="KPH93_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG1689121.1"
                     /translation="MKSFANNITFSRIVFALLMVSSTFYSLPFWMCYFWCGISDILDG
                     FIARKLRQQSVIGAKLDSIADFIFAVTILVTVAKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGASIFAFPLLYICFGLDIAGIMICIMAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(10325..11485)
                     /locus_tag="KPH93_002312"
     CDS             complement(10325..11485)
                     /locus_tag="KPH93_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1689122.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASILTTFSYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISHNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEEKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKSALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(11473..12147)
                     /locus_tag="KPH93_002313"
     CDS             complement(11473..12147)
                     /locus_tag="KPH93_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689123.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
BASE COUNT         4181 a         1675 c         1939 g         4376 t
ORIGIN      
        1 tttattttca tataacaagt ctccttagtt ttaagtttta aattactata aaaaggtagt
       61 tttaaagcac acaattttga aaatatagta gtatatttga aagtataata acatataaaa
      121 catgacaaca gaatgtcatg ttttataaat aatctatcgc aacttagaat ttattagagc
      181 ttctgcctgc tcataatcgc ctttatgaac ataaattaag tactcataag agaaatcttc
      241 cttttgacct aaattgccaa gtgttatttc attaaaaaaa ggttttttag agtaagcatt
      301 atttttcact ttatacttat atttaatttt atttactgat aatatattgc atatctcgtt
      361 aaatctattt aaagaatatc ctcggaaaag ctcagttcta tttaaaatca taaggaaaac
      421 ctccttttaa aagtaatata ttgaaaagca taggagaaat gtactataag ttgttataaa
      481 taaaaggtag caaatttaaa ttgatttgta aagtttttaa gagtattata ttagtgataa
      541 aacaaataat ttttagaatc taactaataa caatttaaaa tagataactt atgtaagaat
      601 cgtaggaact acggagatat cctgtagaga attagtaaga catattttag tatgcaaaat
      661 tctattaagt aggaatctta taactttagt catgtgagat tcataagaat atttaagtat
      721 tgaaaggaga gtttaattga aaaaaatatc tcaagaaaaa ataagtacac atatccaaga
      781 agtaccacag gaaaaaattg aacagtggaa atctgtatat gaaaaatata aaagtatttt
      841 aaaagtaaat tctaagtctg ttttagaaat aatagagttt ttaaaacgaa aatatgtaat
      901 agtagaagaa acttcagagg aatttaaaca tgtagttatc aataatatta agttaaacaa
      961 gcctttttca caaaagattc ccagtgataa agaattaaaa tctattgtat ttacaattca
     1021 aaatgaagga aatgcaataa aattgtacga tactcaagat gaaacttata gtggctgtaa
     1081 gattattgta ggtatggaat ttgaaacagg ttgtgttttt gtagatggta gtgatgagct
     1141 taatgatgaa ataactgctt ttaaaggatt agataaatat gatataaata attattattt
     1201 agtagcaaac tatattagat gtctaaagaa atataaatta atagattagt gtttgattta
     1261 agaattatag agtaaatttg aagttagtgt gataatgaaa ctaagcttaa atctaatata
     1321 actgttaaac ataaacttaa atagagttgc caaaataaaa ttggtaactc tattttttat
     1381 tttaattttt attaaaatta gtaggtgtta tagaattgtt attttttcat gttagtattc
     1441 tcttagtaat agatttaagg tattacaaga agggagaaat taatatgaaa gatttaattc
     1501 ttgaaacaaa aaacttatca aaaagatatg gagaacaaat ggcagttaac aatgtttctc
     1561 tacaaattga acgaaatagt atatatggac ttttaggacc aaatggagca ggaaagtcca
     1621 caacacttaa aatgcttgtt ggtcttcttc gcccaacaga tggacgtatt atttttgata
     1681 gaaagccttg gaaacgagaa tctcttgcaa aaataggttc gttaatagaa tcacctgcat
     1741 tgtatggaaa ccttacagca gaggaaaatt tgttagtaca tacaaaaata ttgggaatac
     1801 ctaaagataa aatttatgaa gtgttggaaa tagtaaattt gaaaaataca ggtaaaaaga
     1861 gagcttcaca attttctatg ggtatgaaac aaaggttagg aatagctatt gcacttttga
     1921 gtgacccaga gttgttaata ctagatgaac caactaatgg tcttgaccct tttggtattc
     1981 aagaattgcg tgaattgatt gcttcttttc caaaaaaagg tataactgta attctttcta
     2041 gccatattct atcagaggtt gctcaagttg tagataatat aggaattata aatggaggac
     2101 gactactatt tcaaggaatg cctgactcta atgaaaatct agaagaattt tttacagatg
     2161 ttattcttaa aggaggacaa aacagatgaa tgctttacaa tcagaacttt taaagtataa
     2221 aagaacattt atgggtaaac ttattgtttt tttcccagtg tcttttgcag catatgcatt
     2281 tataatgcaa tcaacactca tgcaaaatcc attatctcaa acaacttctt gggcgtggca
     2341 aaatctttta gcattaattt ttaactggtg gtcattttta tttctgccta ttggatttgc
     2401 attgtttgct actttggtag ctttccagga aaaaaaagct ggaaattatc gtgctttacg
     2461 tacacacaat gtgtctccta tgacactttg gattaataaa gttatagcta tggcagtcta
     2521 cagttttatt tcaacgctag ttttaatatt cgtaacaatt ataactggtt taatattgaa
     2581 agctggtcca gttccttttg gacagattat aggggcgagt attgtatgct gggttgtttc
     2641 tcttgctata cttccactcc agctatggct agctacatgg aaaggtatgt tcctaagtat
     2701 gggagttgga gctttaggca tgatatttgg tgtccttgct gcgacaaaac cattttggat
     2761 tgcagtacct tggagttggg cagttagaat ggtatgccca atcatcaaca tacatcctaa
     2821 cggtactgta ttagaagctg gagacccact tttgaatact tcagtgattc ctattgggat
     2881 tgttgtttct ttggttgtat ttatagtatt aacagcactt actgcagctt ggttcaatag
     2941 gagggatgac aaatgataaa actattatca tcagaatggc ttcgagcaaa gaggacagct
     3001 gtccagtggc ttactctttg tatgcctatt attttttcat tatgtgtggt tttttatctc
     3061 acaactaaat ctggaagtac acaagaattt gcttttgaag gattttttac agtatggact
     3121 gtattcatca ttcctattgg agtaggtatt ttagcaggat ttatagtgca ggaggaagag
     3181 cttgctggaa attttaatgg ttttttatgt gtaggtattt ctcgtgttag attatatctg
     3241 ggaaaatttc tttttcttct gttttgctta actatatgca catttattgc tacattgata
     3301 ctttgcatag gaatgagtat tgcagtgcca tctggagcaa caatatggtt attcctgtca
     3361 gcagcaggac ttgtggtgat tgggacactt ccactattag ctattcatct ttgggtgagt
     3421 tttgcatggg gtatgggtgc atctattggt attagtattg gtggaatttt gatggcagca
     3481 atacttggag taactagcgt tggtgaaaaa gtatgggcat ttgttccatg gacatggcca
     3541 gtaaaactag gtatgcttcc agggatttat tttattaaag aagctggaac tatttcaact
     3601 gaggtatttt actctaaagt gatgcaaaca gctagtatag agcttatagt agtagcaata
     3661 ggtttgatta tattcttgat aggaggcgtt atttggttta aaatgtggga gggaaggaaa
     3721 agttatgagt agacaaatat tgttttaata ctgaccttcc cataagagaa aggtggtgtt
     3781 atattgagta aaatattgat tattgatgat gaaatggacc tagtaatgct acttgaggac
     3841 gaattgaagg caaaaggaca tgaagtgttg atagcttatg atggtcaggc aggcatagag
     3901 ttatcaaaac aggagcctga ccttattata ctggatatta tgatgcctaa aatgaatgga
     3961 tttgaggtat gtcaggcaat tagagatgat gttttatgcc caattatatt tttaagtgca
     4021 aagcaatcag aaaccgacaa aatcaaaggg ttgacctttg gaggggatga ttatgtaaca
     4081 aagccatttg gactgcgtga acttatggca aggattgaag ctaatctgcg tagggaaaag
     4141 cgttcacagt atatcaatga agagaacaag aggtctactt tgtattttgg gaagttatgc
     4201 ttatatatga aggagcgtgc aattaaaatt aatggtaaga atattgattt aacaaaaata
     4261 gagtatgata ttgttgaatt actagcactc catgcaggac aagttttctc aagagaacaa
     4321 atctatgaaa aagtatgggg gtgtgattct gaaggtgatt cctcaactgt tgttgaacga
     4381 gtaaaaaaaa tacgagctaa gttttctgct gtgacaccag aaaaagaata catttccaca
     4441 gtttggggta ttgggtataa gtggaataaa atgtaaagag agagaggtta ttaaatgaaa
     4501 aatcaatccc ttattgccca atttagacgt acttttattt ttattataat agccagtata
     4561 gttgccacag tgattactta tgtatttgct atttatttgt atatttattc cttaaataag
     4621 gatatctatc caccaaacta ttatgagcga caagttccta gaattgaaaa atacatcaat
     4681 gaaaaaaaca tagctttgtt atcacaatcg aatgaagaag gtttaaaaag aacgattcgt
     4741 ggggatgata tgctatatca agttgtggat aataatggca atattctata tggtactaat
     4801 cccaaaaaaa catttaaaac aaaagaagaa ttatttaata attttatcaa taaaactgtt
     4861 cgtaaaggtg gttatatcta tactgttcca atcaaagggg ataatggaaa gattgaggga
     4921 gctgttattc tattctatca agtcaaaata acctttgcaa atagtagagg gagatttgta
     4981 tttgcagtta ttataatggc tctgttttct ccatttctat atatcgtggg ttttactaga
     5041 tggctttcta agcgctttgt aaagaatatc aatcaaccat tacatctatt gattgatgcg
     5101 tccaataaga taaaagaaaa ggaccttgat tttgaaatcg actattattc agataatgaa
     5161 ctcggtaaac tttgtagtgc cttttctgaa atgaaagacg aattaaaagg ctctttatct
     5221 gcacagtgga agatggagca ggaacgtgtt gaaatggtgg aagctctagc acatgattta
     5281 aaatctcctc tttctattat tttgggatat acagatgcac ttattggtaa taatacagat
     5341 gacaatgaaa aacttcatag gtatcttaca gtgataagag aaaatacaga aaaaagtgct
     5401 gctcttgtcc aaaagatgca atatacgtct gacttagaaa aatcaaatat acaattaaat
     5461 cttgttccta taaatttacc tgagtttttg aggcaaaagg tgcaagacta tgaattacag
     5521 gctcatcaaa aggaagttga acttatattg aaaatgcagg gtaatataca atccccgatt
     5581 caaatcgacg tggataggtt gacacgaatt tttgataata tcatatccaa cagtctgcaa
     5641 tacactccat caggtggtaa tattagtatt actgtaaagg atgaaaaaaa tcgtatttct
     5701 tatgagattt gtgattctgg tagtgggttt agctctaaag atttaaaaaa ggctcttgat
     5761 aagttttatc gaggagatga agctaggcag actaaaggag gacattctgg tcttgggctt
     5821 tatattgtaa aacaattagt ggaacagctt ggaggttctg ttaaaataga aaattcaaaa
     5881 tcaggtggag catgtgtgaa attttggcat agcatttaac tatatatttt aaactaaagg
     5941 ggagtgtctc aaaacgaaat ttttagttcg tgaggcactc tttttttgtg aagttaaata
     6001 tattttcatt atttcgacaa tgaaaaaagg cttatctcta ttttgagaca ggcctttttt
     6061 aaagcttata ttatagttaa aaagatttat tagttagatt tgaaagaaaa tgggtacagt
     6121 cattgataat ttaatttata ttattatctg taaaatagtt ataaattgat atctatacag
     6181 tcaattatat tcattttttt aacttttctt aatggaataa gagtagctat aatacatgca
     6241 agtatagaaa taatagtagc ttgtaaaatt gaagtaattg gtatattagt aaaatcaagc
     6301 ttttctatag ttgccatgtt tataataata gcactgacat acccagcaat tgaaccaaat
     6361 atagcagcaa aaattccata ataaacacct tcccataaaa acatcttata taagctacta
     6421 ttactcatac caatagctct tttaactcca atctcattag tcctagtatt tatatttgta
     6481 tgagttgtgt taatgatgtt taaagtacca atcaatccca caaataatat aactgcccat
     6541 gctagaaatt ctatttgttt aaaactctct ttaagttgct tatctgattc aatatatgat
     6601 atccaatgag agccaggatt attttcacaa atctgttcaa tagacaatct tatttgttct
     6661 aaatctgatt tatctttagc ataaatattt atctggttga acttgttttt atttgtcaag
     6721 gtgttataaa cagaatcata tacaataaca tcaacaccat ttacccagcc attaccttgt
     6781 aaaaagaaca ttttatctac cgtactatat atatctagtt gttttttgtt aattgtgata
     6841 ttatctttag gttttaaatt tgtatattta gtttttacac ctggtgtaga aatagcaatt
     6901 ggatttttta taaaacagat agaaccattt ttaaagtctt ctaatgtaga atctgtaata
     6961 gaaggcatta agtcttttaa agtttgttca tcaattccaa tgatttttaa catctcacca
     7021 ctattttcaa aatttatatc tgtatctatt tcgccctgtt tgtattcaga gtactttata
     7081 aaagaagtgc gattgacgtt tttcatgtta tctattttat ctagtattgg cttatcaaaa
     7141 ccagatattt catttgttaa ggaaaaatct ccttctttta aattacttac tgacctagat
     7201 acatctaata cattagagaa ctcactcagt gcaacgaata ctgttatact cataaataat
     7261 gatagtatgg ttattatagt tcttccttta tttcttttta agtttagcca ggctagatgt
     7321 ctttcaaagt tttttattgt tttttctcgt ttaatttttc ttttgatttt ggtagtactg
     7381 ccagccatag caattttggg agagacatgt gaagcatata ttgctgatgg taatgctgaa
     7441 ataagtgaaa aaatcaaaga tatactagtg cttaatacta atgggaacat atatgctgtt
     7501 gtgtttttat gtataagctc agtaatttct ttattatcat ttgctagaag tatatctgga
     7561 tttaagatat tagtgaccat tccagttatg cctttagaag atataattcc aattacagct
     7621 ccaataggta tggcaacagt acataaaatt aagatttgta aaataactat cttatatatc
     7681 tggtttggtt cagctcctat tgctcttaaa catccatact ctttaatttt ctttgtaaca
     7741 gagattttta aaatattgta gattacaagt ccagaagcca gtaaaaccaa taaggcaaca
     7801 aataaagata ccaatataat catagatata ccatcactat tagaactatt ttcatctttt
     7861 tcaaactgta ttttaagagc atttaagaaa atccaattat attgaattga attgtgagaa
     7921 agatttattt ttttagctaa ttgatttact atttcttgaa acttctcttg ctgttttatt
     7981 ttaaaatcta aagaggataa aatatatctc ttgggtaata gattttctga tgcacctttg
     8041 cctacaatac cgtttactat accacttacg tatcctgtat aatcatcctc taaaatacca
     8101 ctcaatttaa agttagctgt gtaattatat ggtggtattg tatcatttaa aagtgaaata
     8161 tctaaattca taggtatagt cactcctagt cttggtttta tacccataag ctttagtgtg
     8221 ttttcatcta aggctatttc gtttttatct ttaggcaaat ttccagattt taatttcatt
     8281 aatttggggt agttggaaag tcctgtttta tcatactctt ttacaagtac agagatacca
     8341 gaatccttta ttttactact gccaagagtt attgaagtac cagcttgata aactctatca
     8401 tctttcttta tgtcatcaac ttgattttta gtaagttggt gaaaggatac atgtctatca
     8461 ccgttaaaag aacgggcttg ttcaattctt aaattttgta ggataccaat tgactgtcca
     8521 accacagttg tcataatagt tgatagtact attgctataa tgatgaatgt agtcataagt
     8581 ttctggcttt ttaattcttt tattgaaagt gaaaggtaat tcttcatttc tatttcacct
     8641 ccataagaac accatcaata attgaaattt ttctatcagc aagttttgca attctatcat
     8701 catgagtaat taccactaaa gtctttccaa actgttttga agtttttata agaagattca
     8761 taacttcact gccactttta ctgtctaagt ttcctgtagg ctcatcagca aatattatat
     8821 ctggttttgc aataagtgct ctagcaattg caactctttg ttgctgacca cctgaaagtt
     8881 catggggaag atgattgagt ctatctgcaa ttccaagtaa tgatgagatt tcatttaaat
     8941 attctttatt aggttgtttt ttatctagaa gtacaggcat tgaaatattt tcagatgctg
     9001 tcagaacagg taaaagatta aactgttgaa aaacgaatcc aaaggatttt cttctaagtg
     9061 cagatagttc tttttcactg tgtttatata tatctttatc atcaagtaac acttgtccag
     9121 aggtaggatt atctaatcca cttaaaatat gtaaaagtgt gctttttcca cttccagaag
     9181 gtccaattat agaaacaaat tcacctgagt ttatttccaa atttacatta ttaagtgcga
     9241 taactttatt gttattagaa ccgtatattt ttgataattg ttttgctttt ataatcataa
     9301 aaaacactct ccttttctat ataagaagag tgtaagtttt aaaactcaat tttttcccaa
     9361 cttttagttt aaatctaaaa agtcttcact atttacagat aattcgcagt actgataagc
     9421 agtttcagta tcatttttaa tttaaaatgt aatttatgca ctaaaggttt tacaatacaa
     9481 ctctattgct ttaaaactat tttacacata aacttgtgga taatttttta tgtttacttc
     9541 ctcattatta tataaattat ttttgtcaca aattctaagt tgtcacataa atatactttt
     9601 tatatcacga tttaattctt ttgattttat cataattgct atttcttcaa aagaagaaag
     9661 aaaagccatg atacatatca taattcctgc aatatcaagc ccaaagcaaa tatagagtaa
     9721 tggaaaagca aagatagaag ccccagtgat tttattcatc aaagtatgaa gagatgaaaa
     9781 tgtacgatac ttaaaaaaac caatactgta actaatcaac cgtagcaatg ctattgcaac
     9841 aatacaccac catagataca tgggaagagc aatatttttt gcgacggtaa ccaaaattgt
     9901 aaccgcaaaa atgaaatcgg caatactatc taactttgca cctattacac tctgttgtct
     9961 caattttcgg gcaataaaac catcaagtat atcactaatt ccacaccaaa aataacacat
    10021 ccagaaaggc agtgaataga atgtagaact aaccattagc aacgcaaaca caatacgaga
    10081 gaatgtaata ttattagcaa aacttttaat ataatcacct ccaaaattct aatttcgtac
    10141 tattctacat acttaaatat atatttctat tattgtttaa aatacctttt accaaagtat
    10201 tataattgaa gcaactctta caacttctac aaaatagtat atattggttg taaattgaaa
    10261 tacattttat aaattctaaa ccaacaaaac ttatataaga gcagtgaaag cttacaaaat
    10321 cattttaatt tcttgttgag aatttagcag taattttagc actgttagtc gaattatttt
    10381 caatagaaat agagccacca tgtttttcac ataatatttt tgaaatataa agacccatac
    10441 caaagtgtgc attgctgtca tttaaagtct tatctctata aaaaggtctt aaagctgatt
    10501 ttagagattc tttactaaat cctataccat catcttcaat tacaaaggta aggagttctt
    10561 gttcaagata tataagtatg gatacattat ttctagcata tctaaaagca ttcgatatta
    10621 tattttcgac gactctaaga atgatatttt catcaaaaaa tagatttata ttatttattt
    10681 gacttttaag tgtaaaagat ttttcttctt gtttagcaat gatagatata ttttcatcta
    10741 agttttctat aaacttgttt atattcgaca tagattttga aacaactaaa tcttctaatc
    10801 gttgagcatt actcatattt tctacatagt attcaattct gttaatatgc tcagacataa
    10861 gttgtgtggt tgataaaatc ttctcatcac taagcttacc tgttggtatg tattttgtta
    10921 aatagtttga gtaacctttt agaactgtta ggggatttct taaatcatga gaaaaagcag
    10981 cattaagttg ttttctatca ttaatcatag tccacatctt tatgttgttt ttatttagtt
    11041 cagacctcat tttttcaaaa gcattacata gattacctaa ttcatcatta ttattatatt
    11101 ctaaacaaaa gtctaaattg ttgtgggata tttccattgc acctttattg agtatttcta
    11161 taggtgtttt tagttttacc ttgtaaaaaa ttatatcagc taataataaa agtcctataa
    11221 aaaatataag tggaagtaca agctgaagta tgttgattat attaagtaat gttttatcat
    11281 aatcactaaa ttggttcaca ttgttttgaa ctatggttat aatacccgta tttataggtg
    11341 tttttaaata cttatcttgt atagaattat atagattatt aagcaaaatg tatgagaaag
    11401 tagtaagtat tgatgctata attagtgaag ataaagtaag aacaaagaaa gcttttttta
    11461 atgacatatt atttatttta tccatttata accaactccc cagactgttt ctatataatt
    11521 ttcaagagta tgttttgata gtttgattcg tatctttcta atgtgttcca ttataacatc
    11581 actatttcca ttgctattgt atccccagac tctatcatag attttttctt tatcaaatac
    11641 ttgtccagga ttcatagaga gaagctctac tatatcaaat tccttttttg aaagtgaaat
    11701 ctgattatta tctatacaaa ttaacctttt ttcgtaatct atcgttaaat cattataaaa
    11761 tttaatttta gtttgatttg acttcctttg ttcacgtctt aaatgggcag atactctagc
    11821 tccaagttcg tctaaatcaa aaggttttac aatataatca tcagcaccag cacgaaaacc
    11881 atttatcttg tcagtatttt caattctagc tgtaagaaat agaatagggc atgatacata
    11941 atctctaatt ctattacaaa cttcaattcc atctatttca ggcatgttta tatcaagtag
    12001 aatgatatct ggatttttgg atattttttt taatgcgtct tttccacttt gagcagtgta
    12061 aacctcataa ccattcattt caaagtaatc ttttaagagt gttattatac cttcctcatc
    12121 atctacgacg agtattttat tgttcataag aatgctccct tctaaattaa g
//
LOCUS       DAEMAE010000110        12032 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEMAE010000110 DAEMAE010000000
VERSION     DAEMAE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.112"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..529
                     /locus_tag="KPH93_002314"
     CDS             167..529
                     /locus_tag="KPH93_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG1689124.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            594..1211
                     /locus_tag="KPH93_002315"
     CDS             594..1211
                     /locus_tag="KPH93_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG1689125.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEST
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1208..1693
                     /locus_tag="KPH93_002316"
     CDS             1208..1693
                     /locus_tag="KPH93_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG1689126.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1736..2254
                     /locus_tag="KPH93_002317"
     CDS             1736..2254
                     /locus_tag="KPH93_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG1689127.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEILI
                     DELINGFDCTIM"
     gene            2390..3349
                     /locus_tag="KPH93_002318"
     CDS             2390..3349
                     /locus_tag="KPH93_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1689128.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMAELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKENKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3375..5078
                     /locus_tag="KPH93_002319"
     CDS             3375..5078
                     /locus_tag="KPH93_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG1689129.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5249..7351
                     /locus_tag="KPH93_002320"
     CDS             5249..7351
                     /locus_tag="KPH93_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG1689130.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEREKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLENTDEKEVINSNFAYKED
                     SDVVKIIFEQDCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKENKEIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKGYQN
                     NYTEDELQEDVLNEKIDIEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFILPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNNGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7365..7805
                     /locus_tag="KPH93_002321"
     CDS             7365..7805
                     /locus_tag="KPH93_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG1689131.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVITI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSM"
     gene            7841..8644
                     /locus_tag="KPH93_002322"
     CDS             7841..8644
                     /locus_tag="KPH93_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG1689132.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            8684..9280
                     /locus_tag="KPH93_002323"
     CDS             8684..9280
                     /locus_tag="KPH93_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG1689133.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMIAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9386..10249
                     /locus_tag="KPH93_002324"
     CDS             9386..10249
                     /locus_tag="KPH93_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG1689134.1"
                     /translation="MKFFKVDTKARVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYTEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10316..11305
                     /locus_tag="KPH93_002325"
     CDS             10316..11305
                     /locus_tag="KPH93_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG1689135.1"
                     /translation="MLLLKKDDIEKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDKAIDNADVIVLATPSKQPVFNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGMDWKW"
     gene            11396..12007
                     /locus_tag="KPH93_002326"
     CDS             11396..12007
                     /locus_tag="KPH93_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689136.1"
                     /translation="MKMVKKLILSSIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         4867 a         1184 c         2280 g         3701 t
ORIGIN      
        1 ttttgttgac aaattatgtt aagagaatta agtataatat gacataaata attactttta
       61 tttatcaatg aaatgtattt attgtgtagc gaaaaatagt cagatagtat agacaataac
      121 tgaaaataaa agcgtttatt gttgtacata tggaggagtt agttttatgg aaaataaaat
      181 tttgatagta gatgatgcta tttttatgag aatgataatt aaagaagctc tgagtaaaag
      241 tggctataat aatttaatag aagcaaatga tggagaagaa gcgtgtaata tatttaaata
      301 tgaaaagcct gatttgatgt tgcttgacat tacaatgcca aaaaaagatg gtttagaggt
      361 gctaagagaa ataaaaaagt tggattcaag tgctaaggtt gtaatgtgct ctgcaatagg
      421 acaagagaat atgattgtag aagccataaa acttggagcg ttagatttta tagtgaagcc
      481 cttcaaaaca gaaacattag ttaaagttgt aaataatgtt ttaaaataaa agcgtaaata
      541 tacactgata caatgaaaag taaataaatg caataataaa gggaggggtt tatattataa
      601 attatttaga acttgatgat gttcatatag atgctcttag agaaattggt aatataggct
      661 ctgggaatgc aattactgca cttgcatcca tgataaatag taatgtagaa gtattaatac
      721 ctatggtcaa gatacttgaa tataatgaag cgacaaattt gcttggtggt ccagaaaata
      781 aagtagtatg tattttactc gatatgaaag gtgatatcaa tggaatgttt atgtttttgt
      841 tggatgaatc aataactcaa cttatgctaa gttctctttt taataaagaa gaatctactt
      901 tagatgagat tgaggcaatt gaaatttcag caattaaaga aataggaaat attatggcaa
      961 gttcatatgt aaatgctatt gcatctatgt taaacatgac tataagtgtt tcaattccag
     1021 atatatgtat tgatatggtt ggagctgttc taaatgtgcc tatgataaga ttttcagatg
     1081 ttggtgacaa agtcttattt atagaaaata aatttaaaat gagtgataat tattttacta
     1141 gtcacatttt gatgattcca gaaatgagct cacttagaga aatactcgtg aggttgggat
     1201 tagaagtatg aatagagaga tagtagtgaa cattgcagat atgaagatag cctatagacc
     1261 aaatgtttta gttacttatg cacttggttc ttgtgtagga gtatgtttga tagataaagt
     1321 agctggaatt ggtggaatgt tacacgttat gttgccatat agtaaagatg ctattaacat
     1381 tgaaaataag tgtaaatttg ctgacacagg aataaatgag ctcattaaat ctatggagaa
     1441 tataggtgca aatagaatat atataaaagc aaaaattgct ggaggggctc agatgttttt
     1501 aggaagtagc aattcagtaa ttgcaagtat aggacatagg aatgttgagg cagtaaaaaa
     1561 aactctttta ggattaaata ttccaatatt agctgaggac acaggtatga attatggtag
     1621 aacaatacgt ttttatactg agacgggcga attacttgta gattcaataa ataaaggtaa
     1681 gaagaaatta taatatatat ttcatagaaa gtgtatttta ggagggaggt attaaatgag
     1741 atttccagat gacccatatt tagattatgg actaattgat gaaggaatta agtatctaaa
     1801 gtttaaagta gatgagcaag attttggggt tgaacttgaa aaggtaatag aaattactgg
     1861 aaatcaagaa attatattta ttccagaact acctacatat tctaaaggca taataaatct
     1921 aagaggaaaa atcattccaa taatagatat gcgtttgaga cttaaaaaag acgaattaat
     1981 gactacaaat tgcatgtata tagtagtaac tgaaataaag gacttagtag ttggatttgt
     2041 ggttgataga gttgatgata tagtaagcct agaaaaaagt aaaatatcac ctccaccaag
     2101 agttactata gaatattcgg attattttgt aagtggagtt ggagaattag aaggtaaaat
     2161 aattacttta ctagatttag agaaactttt gacagataaa gatgaaattt taatagatga
     2221 attaatcaat gggtttgatt gcactatcat gtaatatagg gatatagttg gaatgttatt
     2281 ataaaaagat tgtgatgaga acatttaagt atatagtggt ataattatta agtataaaaa
     2341 ttttaaatca ttaaaatttg aatatattat gtttaagaga ggtttgtata tgaaaaataa
     2401 gcaacaggga acaattttag tcgttgatga tagtgttctg atgtgtgact taatagataa
     2461 aacacttaca aaagataatt ttaaggttaa aaaagcattt aatgcaatag aggcaaaaga
     2521 atttattgaa gaatttatac cagacattat tttgcttgat ataattttac cagataactc
     2581 tggttttgag ttttgtaagg aagtaaaatc taatgctcgt acagcagata tacctataat
     2641 ttttataact tcaaaaaata cagatacaga catagtaaaa ggatttggtg taggagcaat
     2701 tgactatatg gtgaaacctt ttagtatggc agaattaaaa gctcgtgtta tagctcatct
     2761 tgaagtaaag aaatctcaag ataaattaaa aaagataaat aatgaactgg aatcttctct
     2821 tgaaaaattg aatagactcg ttgtaagaga ttatcttaca ggtctttaca ataggcgaca
     2881 tataattaat cagcttatgg agcaaagaag aattaataaa tatagtgaaa catcaatatc
     2941 ccttattctt ggtgatattg atgactttaa ggttatcaat gatacttatg ggcatgaagc
     3001 aggggatttc gtgttaacag taatatcaag tataataaaa aagaattgta gacaaattga
     3061 cactgtatca agatggggtg gagaagagtt tttgataatg ctaacaaata ctccaataga
     3121 aggtgctaag atactttcag aaagaatccg taaagagata aaggagtttg atttcaacta
     3181 taaagaaaat aaaataaagt gcactattac attaggagta gtagaagtaa actcaaatat
     3241 atcattagaa gaaaacatat ccttagcaga caaagcaatg tatgagggta aaaacttagg
     3301 aaaagattgt agtgtagtga gtacaatgaa aggattaaaa aagatataaa cttactttat
     3361 aaaggagtac agatatgaaa agagaagtaa agctttctaa attagcaatt gtattgctga
     3421 ttattatagt attggctgtt ctagcaggga ttttttcttc tatttcatct tataaagcat
     3481 ttgaaaaagt agaaaaaagt attaatgata tgaataattt gagaactcaa agtcagtata
     3541 taaaagatac aactagagaa ataagtgatg tattaagaag atacgtattt acaggagaaa
     3601 agaagaataa agatatttat gaaaaagaat tatacggaaa aacaagagaa tcagctttaa
     3661 acaaattaga aaaaattggt cttacaaaga gcgagtcaaa taaaatctta gaagcaaaag
     3721 aaatatcaga ttctttagta ccaacagaac aaagtgcaat aaaggcagtc gaaagtaatg
     3781 ataagagtaa agctcaaagt ataatttttg gtagtgacta tataaactcc caagagaaaa
     3841 tggattcttt aataaatgaa tttcaagagg atatagttac ccgttcaaat tccaaaattg
     3901 tagaagtaaa aaaagaatta caaagtctaa tattaagaac aatatttata ctttctcttt
     3961 taataatatt gacaataata gaatatacag taataaaatt aaaaatagtg attcctatta
     4021 ataaagtaga aaaacacttt tcaagaattg catctgggga cttaagaaca agtatagacg
     4081 ttgaagaaag taaatctgaa atagggctat tagtgagttc tgtcaaaaag atgcaaagta
     4141 tgcttttaaa ttacatagac cttatagaca gtacattaac tagtattgca aaaggagatt
     4201 taagagtaga gatagaccaa gaatttatag gtgattttaa atcaattaaa atatctttag
     4261 aaagtataat aaactctttt aatggtgatt tttctgaaat aaatttatca gcagagcaag
     4321 ttgcaagtgc atcagaacag gttgcagcag gtgcacaagc tctttcacag ggtgctactg
     4381 aacaagctag ttcagtagaa gaattagctt ccacgattaa tgagatatca gatggaatta
     4441 agaagtgtgc agatagtgct agtcttgcat gtgaggtatc tgaccaatct gcatcacaag
     4501 ttggttttgg aagtagatgt atggatgata tgatgacatc aatgggtgaa ataagtgaaa
     4561 aatcgagtga aatatcaaaa ataatcaaaa caatagatga gatagcattc caaactaata
     4621 ttctcgcatt aaatgcagct gttgaagcag caagagcagg tcaatatggt aggggatttg
     4681 cagttgtagc agatgaagtt aggtctttgg ctggtaaaag tgcaaaagca gctcaaaata
     4741 cagctattct catagaagaa gctattaaaa cagtagaaaa tggtgtcaga attgctaaag
     4801 agactgcaaa tgcattggac ttggtagtaa atggtgtaga aaaaactact aattatatta
     4861 atggtatttc ttcggctgta catagtcaag aagtagcaat tacacagatt ttatctggaa
     4921 tagagcaaat ttccatagta gtccaaatga actcagcaac agcagaggaa agtgcagcag
     4981 cctcagaaga gatgtctgct caagctcaaa tcttaaaaga attagtagaa aaatttcatc
     5041 taaaggaaag tgaagtaaga gaatcatcat gggtttaaat tatgagatgc atatatatag
     5101 tagaataaga gaatgattgt gtatttaaat tatgaggtac atatagtgag ataagagaat
     5161 catcaaggat ttaaattatg agatgcatat ataatagaaa attatttgta ggtcgtaggt
     5221 gataaatgtg aattatattg aaccgagtat gagacaaatg ttagatatgt ttatatttga
     5281 gacaagtcta ttacttgaac aattagatga aattatgatt caaatagaaa gagagaaagt
     5341 atttactcat aacaacatta atgaaacttt tagaataatg catacgataa aaggttcagc
     5401 atctatgatg gggttagaca atatatctgc tttagcacat agtgtagaag acttgtttta
     5461 tgctatacga gaaggtaaaa tagaagtttt agacaatgac ctattatttg atttagtctt
     5521 tcaatcttta gattttataa gaagagatat agatgagtta caatcaagag gttgtatatc
     5581 ttctgattac tctaatttta ttgaaggaat tgaagagtat gtgagtactt tggaattatc
     5641 aagttctatg aaatcaaagg ataatgcaaa tttagaaaat acagacgaaa aagaagtaat
     5701 caattcaaat tttgcataca aagaagactc tgatgtagta aaaataatct ttgaacaaga
     5761 ttgcatgatg gaaaatgtga gagctttcat gcttgtattt gaacttagag attactgtga
     5821 atttatagat ttttatccaa aagatgtaga aacaaatgca gagtctacag aatatattaa
     5881 aagttatggg ttttatatac aatttgtagc taaagagaat aaggaaatag tttataaaac
     5941 tatagaagat aatctctatg taaaagaata tataaatatt agtaaaaaag aaatagaaat
     6001 gaaaagtaca tcaaacaatg atgcagtaga aaataaatta aaagataata ataaagtaag
     6061 taaaggttat caaaataact atacagaaga tgagttacaa gaagatgttt taaatgaaaa
     6121 aattgatata gaaaaagata gtctaagtga agaaaatgaa catttaccaa taagtaagca
     6181 gagtattatg agtgtaaatt tagaaaaatt ggacttactt caaaatatag ttggagaaat
     6241 tgtaattatg gaatcaatgg ttgtaaatag cgtaaattct acaggtgttg atatagataa
     6301 tttcaataag gcaacaagac aattacacaa gcttacagat gaattgcaag atatagtaat
     6361 gtctatgcgt atgattcaaa ttgctggtgt atttcaaaaa atgcatagaa ttgtacgaga
     6421 tatgaataaa aaattagata aaaatgtaga gttaataact ataggagcag agacagaggt
     6481 agataagagt gtaattgata atttagcgga accttttatg caccttataa gaaatgctat
     6541 ggaccatgca atagagccta ttgaggagcg tatagcgaaa ggtaaagaag aagtgggaaa
     6601 aatagtttta tctgctgaga atttaggcgg agaagctgtg ataagtattt cagatgatgg
     6661 tagaggcatt atgagagata agattttggc aaaagctaaa gaacaaggtc ttcttaaaca
     6721 tcaaagtaat gagtatactg atgacgaggt caattctttg attatgactc caggtttttc
     6781 tacaaaagaa tctataactg aattttctgg aagaggagta ggtatggata ttgttcgtaa
     6841 aagtattgaa aaggttggag gttcaattga agtaaaaagt aaacaggatg aagggacaac
     6901 ctttactata aaaataccat taactctttc tatagtagag ggtatgaagt ttaaagttgg
     6961 agattcacta tttattttac caatatcatc aataagaaga acatttaaac ttacagactt
     7021 aaatcaaata atcacagagg gaaataaaac agagttaatt acaatatatg gagcatgtat
     7081 tcccattata aggttacata aaatatataa tattcaaaca aaagtaacca atctgatgca
     7141 tggtaatatg ataattattg aaaatgagca taaagcagta tgcatttttg ttgatgaaat
     7201 aataggtaat caacaagttg ttgtgaaacc atttccagtt tattttaacc gatttaatat
     7261 aaaaaataat ggtttatcgg gatgtacaat acttggggat ggaagtataa gcttaattat
     7321 agatgcagat agcattatag aaatatatta ggggatttta aagtatggct aaagatgaaa
     7381 ttattaaaca agtattaaca ttttatgtca atgatgttat ttatggaata gaattagaaa
     7441 atgtgattga gaccataaga tttcaatcaa taacgtatgt accatgtctt ccaacatata
     7501 taagtggagt tattaatttg agaggaagaa taataccagt tattaatatg catataaagt
     7561 acaatcttcc taaagctgat tataatgaga gaacttgtat aataattaca aaggttgatg
     7621 agtatcaagt tggaattatt gtagataaag ttgttgatgt tattactata gaaaatctaa
     7681 atttactaga gacgactaat tctaacaaca ctaatattaa taaagatata aaagagattg
     7741 cgaaagttga agataattct atattaattt tagatattcg aaagtttttg attaatagca
     7801 tgtagttagt tctgataaat tagcaggagg aagtttttgc atgataaaac tcacagatga
     7861 agaatttata gttcttgtaa accatgtaaa aaaagaatat ggtattgatt taagtaaaaa
     7921 aagagcgtta attgaaggtc gcttatacaa tacaatgata gagaaaaaac ttagttcttt
     7981 tagtcaatat atgaatctat tatttaaaga taagacagga aatgaagcta taaacctaat
     8041 aaataggttg agtactaacc acacgttttt tatgagagaa cctcaacatt ttgagtttat
     8101 acagaatagt atacttcctt tttgggaaga aaataacaaa ataagaaaca taaatatatg
     8161 gagtgcagga tgttctagtg gagaagaagc gtattcaata gctatgactt tggatgatta
     8221 ttttggatat aataaagagt tgtggaacat taaaatcata gccacagata tttcaactaa
     8281 ctcacttgaa aaagctaaaa gaggtatata catagaatca aatgttaaca atgttcctga
     8341 gttatggaag aagaagtatt tcatagacaa taaggatggt acatttaaaa tttgtgataa
     8401 gattagaaaa agtgtaattt ttaagccatt taatttaatg aatgattttt catatcaaca
     8461 ttttgatttg attttttgta gaaatgttat gatttatttt gatttaaaaa caagagaaga
     8521 acttataaat aaattttata atgctaccaa aaaagatagt tttttgtttg taggacatgc
     8581 agaagtaatt aacagaagta gtactaaata caaatatgta aaacctgcta tatataaaag
     8641 ataaaaaggt atttttgata gatgattttt tggaggcggt aatgtggaac gttttaataa
     8701 tatggttatt gctataggcg catctgtagg tggtacagaa gcaatacttg aaattataaa
     8761 agacctacca aaatcaacac cagggattgt tgtagttcaa catatgccag ctatatttac
     8821 ttatatgtat gcacaacggc ttgacaagca gtgtattatg aatgtaagag aagctaagaa
     8881 taatgataga gtcgaacaag gaaatgtgtt aattgcacct gggggttacc agatgaaatt
     8941 gtgtacggat aaacaaggat attatgtgac ttgtgaaaaa ggagaaagaa taagtggtca
     9001 ctgtccatca gtagacgttt tgtttgattc agtagcagaa gtagcaggta aaaattcaat
     9061 aggaataata cttacaggta tgggttatga tggagcaaat ggacttttaa aaatgaaaga
     9121 aaatggttca tttacaattg gacaggatga aaattcttgt atagtatatg gaatgccaat
     9181 gatagctttt gacagaggtt ctgttatgtt acaattacct cttggtgaaa tttcaaactg
     9241 tttgataaga catattaata ttttaaaaca aaaagattaa ttttttataa ataaaaatta
     9301 atcttttatt aaattatatg gataaaaaac cctagaaact ccaaatataa taagagagat
     9361 ttatatttag gaggaaaatg ataatatgaa attttttaaa gtcgatacaa aagctagagt
     9421 tttaagatat gtatttgttt taacattgac tataatggct gttttggcaa ttaaacatga
     9481 ctataaatat aataatttaa ggaaagatgc ttcaattaat ttaaatagta ataaggaagc
     9541 agtgactgtt atgagtaacc agtcattacc aaagggtaat acaaatagta aacaaacttt
     9601 atcagtaagt acaataggaa gtaaaacaat agtgaaaaat gatgagtatt tagaaagtac
     9661 tataaacatg cctgtaataa caaattcaaa taaaattgtt gaaaggtcta caaatgataa
     9721 gataaaaaat gatatatttg aattttataa taaatcatat aaagaagcaa agcagtatct
     9781 taaggataat cctgatgaga aaaataagtt tgttgcaaat gtagattttg aacttaaaaa
     9841 gaatactgat tctgctctta gtataaaagt gagatattat acttatagtg gtggagcaca
     9901 tggattttat caagatatag catataatgt ggatatgaga actggaaaat ttttagaatt
     9961 aatggactta tttaaggata atactaaata tacggaagtt atagacgaag aaattaaaag
    10021 acaaatagct gaacttgaaa aaaaggatga agaaaatatt ggaatatata attttaaagg
    10081 tataaaagag aatcaaaact tttacttgca agatgagaat ctagttgtat attttgattt
    10141 atatgatata actccatatg cagcaggaat accagaattt tcaataaata aagcgttgat
    10201 aagttccatg ctaaaaccag agtatgtaga tttatttgat ttgaaataga agattaaatt
    10261 aaatgttatg ctataatatt tagtatagag acttattaaa attgggggta taaaaatgct
    10321 acttttaaaa aaagacgaca tagaaaaagt atttacaatg agagatgcaa ttgaagctga
    10381 taaggaagct tttagaattt attgtgaagg aaagagtgta aatcctttga gaaccaatat
    10441 atctgttcca agtcaagatg ctagtatgtt atttatgcca ggatacgtag aagaattagg
    10501 ctgtggggga ataaaaattg tatctgtatt tcctaaaaat gctcaaaaag gcaaaccagt
    10561 aattccagca acagtactat tattagatgg agaaactgga gaagttagtg cagtgttgga
    10621 tggtacttat gtaactcaga ttcgtactgg agcagcatct ggagcagcta tagatgtatt
    10681 ggctaaaaaa gattctaaga ttggagcttt gataggtcta ggaggacaag gtgagccaca
    10741 acttgaagct atgatagaag ctagaaattt ggatgaggta agagttttta gtagaaacaa
    10801 ggaaagtaga gaaaaatttg cagaagaaat gaacttaaaa ttaagtaaat ataatactaa
    10861 aatagttgca gttgaatcat ctgataaagc tatagataat gcagatgtga tagttttagc
    10921 tactccatca aaacaaccag tatttaatgg aaacttagtg aaaaaaggag ctttaataag
    10981 tgcagtaggt tcatatatgc cggacatgca agagttgtgc ccaaactgtt taactagagc
    11041 atctaagata ttttttgaat caacagatgc agttttatct gagtcaggag atatattaat
    11101 tccattaaaa gaaggtaaga taagtaaaga tgattttagt ggtgatttag ggaatgttat
    11161 aaacggaact attcctggaa gagaagatga tgatgagata attgtgttta aaactgtcgg
    11221 aataggtgta caagatgtcg taacagcaaa aagaatatac gacaaagcaa aagaaaatgg
    11281 tatcggaatg gactggaagt ggtaaaatat tcaaaataat tctatagtta gacaaattta
    11341 tgttataatg ttgataaata aattttttat tattttaatt gagggaggat gttttatgaa
    11401 aatggtaaaa aagttaatat tatcttcgat tatggctcta gttttagttc taactatagg
    11461 gtgttctaat gcagaagaaa aagtaaagtc gaattttgaa gctaatttag ctgcattaga
    11521 aatgtataag tatgatgaaa aaggaatttc tgatgcatta acagaagctg aatataaaca
    11581 gattcaaact tataacagca aggataaggt ttcaatgatt gctcagttga cacctcttta
    11641 tggtaaagaa aatgctgaaa agatatacga aacaattgtt aaattaattc aaggtgttga
    11701 aaagacagta acagtggaat cttctagtaa ggataagata actttgaaag ttgtaagtaa
    11761 agctttagat actagtaaag taatgagtga agcgaacact ttagtaaaat cttatgtaac
    11821 tgatgaaaag aattacgata aaaaagaaaa agaagttcaa gatgatatga caaaaatgat
    11881 tatagacctt tacaataaga aaccaactaa ggaagttaca actatgatta catatactaa
    11941 aaaagatggt aaatggcaac cacctagtaa tatacaaaag ttatgtgaag gaatatatac
    12001 aatttaattt attttaaaga cacaaaacta tc
//
LOCUS       DAEMAE010000111        11927 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEMAE010000111 DAEMAE010000000
VERSION     DAEMAE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.113"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            173..808
                     /locus_tag="KPH93_002327"
     CDS             173..808
                     /locus_tag="KPH93_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689137.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            818..1522
                     /locus_tag="KPH93_002328"
     CDS             818..1522
                     /locus_tag="KPH93_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG1689138.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            1562..2020
                     /locus_tag="KPH93_002329"
     CDS             1562..2020
                     /locus_tag="KPH93_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG1689139.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            2039..2296
                     /locus_tag="KPH93_002330"
     CDS             2039..2296
                     /locus_tag="KPH93_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG1689140.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            2306..3091
                     /locus_tag="KPH93_002331"
     CDS             2306..3091
                     /locus_tag="KPH93_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG1689141.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILSLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            3126..3653
                     /locus_tag="KPH93_002332"
     CDS             3126..3653
                     /locus_tag="KPH93_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG1689142.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    3828..4060
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4152..7259
                     /locus_tag="KPH93_002333"
     CDS             4152..7259
                     /locus_tag="KPH93_002333"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG1689143.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLANKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFDEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     gene            7555..8427
                     /locus_tag="KPH93_002334"
     CDS             7555..8427
                     /locus_tag="KPH93_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1689144.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            8541..8813
                     /locus_tag="KPH93_002335"
     CDS             8541..8813
                     /locus_tag="KPH93_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689145.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(8976..9548)
                     /locus_tag="KPH93_002336"
     CDS             complement(8976..9548)
                     /locus_tag="KPH93_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689146.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKNDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERMRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            9732..10574
                     /locus_tag="KPH93_002337"
     CDS             9732..10574
                     /locus_tag="KPH93_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG1689147.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLESYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(10677..11753)
                     /locus_tag="KPH93_002338"
     CDS             complement(10677..11753)
                     /locus_tag="KPH93_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1689148.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         4691 a         1339 c         2065 g         3832 t
ORIGIN      
        1 tataatacaa ttatatataa ataatgcagt cttgtatagg aagattgata aaaaataaat
       61 ttaatatgga aaaaataatt attagaacgt aaaatattag cttataaaaa aggaataaac
      121 attttttaga agaagtactt ataatgaaag taattttata gggggagagt ctttgaaaaa
      181 ggtaaaatat accagattag tgatacttgg tattcttata tatttagtat ttaatatatt
      241 agtaatgata attagcaaaa atgtagatac gttggtgtta aaaaatgaaa aagttgatgc
      301 aaagattcat gtaaaaggaa ctatagaaaa agatgccaat gaccaaagta attatctatc
      361 tacatacatc agtaaagaag attctaataa ctttgaaaaa aatcaaaatg taataattga
      421 atatcatgag caaaaattag atgcaaaagt ttacaaaata tatgaaaata ataataaaat
      481 aatggtaaaa ttaaaaataa gtaaccaaat tgttgcaaat caggatacaa gtgtcgaaga
      541 atttgatata atatataatc agatggaatg tttaaaaata cctaaaactt ctataaaaac
      601 aaaagacaat aaacgtggtg tatatgtaat tgatgaacaa tcacaaagtg taaaatttgt
      661 catattagaa ggaataactt atgagaatga atcttctgta tttgtggatt attataaaaa
      721 tgacataaat ggagttacaa gtgttaatct atatgacaaa ataattttaa gacctaacag
      781 tataaacaca aatataagga taaagtaggt gataataatg ggttctatta aagataattt
      841 gtctgagata agaaacaaaa ttgataaagc tgcaaaaaaa gtagatagag acagcaaaga
      901 gattaccctt ttggcagtca caaagactgt tgatgtagac ttagttaatg aggcaatgag
      961 ggaaggaatc acttctgttg gtgaaaataa accccaagaa ttagctagaa agtatgatat
     1021 tataggtgac aaattaaact ggcacctgat aggaactttg caaaccaata aagttaagta
     1081 cataatcgat aaagtttgta tgatacactc tttagataga attgcattat gcgaagaaat
     1141 tcaaaaacgt gcagaacaaa taaacagaga aatagattgc ttagtacaag tgaatatatc
     1201 taaagaagaa tctaagcatg gacttgatag agaagaagtt gtagactttg tcaaagaagt
     1261 gtcagaaaaa tataaaaata taaaaataaa gggtctaatg acaatggcac cttttataga
     1321 agatgaagat gagataagag gtgtatttaa aggtcttaaa gatttatcaa aacaaataaa
     1381 agaactaaac atccctaatg tagggatgga tgttttgtca atgggaatga gtcacgatta
     1441 tgaaattgct atagaggaag gggctaccat tgttagagtt ggaacatcaa tctttggaga
     1501 aagaaactac aataaaaaat aattactcaa atttgaataa attttaagga ggaagttaat
     1561 aatgtcaaat ggaataatat ctaaatttaa gaattggata gttgatgaag aagaagatta
     1621 cattgaagat gaatatgaaa gtggcatgga cgatatagtt caagaagaag aaatgaacag
     1681 tggctttagt actgcaaaag caaataaaat tgtaaattta catacaacta gccaaatgaa
     1741 agttgtaata gttgaaccta aggtttatga tgaagctgca actatagcag accatctaaa
     1801 acaaagaagg gcagttatag tgaatttgga aggattgact aattctgaag ttcgtaaatc
     1861 tatatttaac tttatgaatg gggcagttta tgtcttagat ggaagtatac aaaaggtttc
     1921 taaatcaata tttatactag ctcctaataa tgtggatata gatgcaaata tgaaaaaaga
     1981 attagaaagt aaagcattct tcccttggca aaataaatag taaaaaggcg gttaatatat
     2041 gggaacaata agaattgcac tttattatct tttagatata atagcatgga tgatagttat
     2101 aaaaagcttg atgacttggt ttccaaatgg aagacaaagt aaaatttttg atattttgga
     2161 aaatcttaca gagccaatag aagggccaat aaggtctata atgtataaat atacaaatgg
     2221 acctatagat ttttctcctt taattgcaat agtattattg atgttcctaa gacaattagt
     2281 attggttata ttctaaggtg ataggatgga taaattaaaa ctaacaaatc acataaagga
     2341 tatagacttg aaaaataaaa tgtttaaagt gatagacaag gctaatagtt gtataaaaaa
     2401 ctatgatgtt aagtctacag agtttcttaa tccatatgaa gtgaaaaatg ctgtagcaat
     2461 attaaattct acaaatgaaa taaaatacag tgtggatgga ggttatgaac aagctgaaag
     2521 aagcactgta tttatttatc cattttacat ggaatacgaa gatatagaag atactcttag
     2581 atttttacaa atagaaggga atttcaaatt taaaaatata tctcataaag attacttagg
     2641 gtcaatactg agtctaggta taaaaagaga aaaaattgga gatataatta tacatgatag
     2701 cttttgtcaa gtagttgtaa gtagtgatat ttgcgatttt ataattgtaa acttagaaaa
     2761 ggtttcaaga aataatgtaa ttgtaaagga aatttccaga gaaaatattg ttaatagttc
     2821 ttcaaaatat aaagaagtat catttacagt atcttcagat aggttagatt gtgtgataag
     2881 tggtatttac aatatatcta gacaagatag tgcaaaatac ataaatggag aaaaagtcca
     2941 tgtcaattat gaaaagataa cttcaacttc taaaatagta aaaaatgatg atttgatttc
     3001 tattagaggc aaaggaagag ctaaggtaac acaaatagga gatattacta aaaaaggaaa
     3061 aataaaagtt caagcaagat taattgtata aggactaatt taaattatag ctgggaggaa
     3121 atgtaatgct aactccaatt gagatagaaa ataaagagtt taaaaaaggt ttaagagggt
     3181 atagagatga agaggttgat gaatttttag atatcatcaa ggaagattat gaatctctat
     3241 gtagggaaaa tacagctctt aaagaaaaat tagggttata tcaagaccaa gtaaacaagt
     3301 atgaaaatat tgaagaaaca ctaaaagcta ctttaataac tgctcaaagt gcagcagaag
     3361 acacttgtag cgcggctaat aaaaaggcta agataattgt tgaagaagct gatttaaaag
     3421 ctagacaaat tatagaacaa gctaataacc gtgttataga aataagaaaa gagtatgatg
     3481 ctttagtcaa agaatttaag atatttagaa ataaatttaa atcactgttg gaagatgaaa
     3541 taagaagtgt tgatgagatt ttttatgatg tagatgaaaa atgtgtaaat gcatttgaag
     3601 gaacaacagt gtataattat agtaatgatg aagctgctac aactttagaa taagcttgta
     3661 atttgtttat tttttatgta gaatcattgt gtaagtttga gaaatctata acaaagatta
     3721 aaaataggat aaaaatgtaa gaaaaataga aattattata tttttcaaaa atatattgac
     3781 tattcaaatt atttgttata gaatttcata taaacacaga taaattttta aaagcgttga
     3841 tagagacatt tatctttaga taattttaca gagaattggg tatgctgaga accaatgaat
     3901 ttataaagat aaaaatcact cttgagctgc aggctgaacg attaaattct actaagctgt
     3961 gccggttagt accgttatac tattagagtg ttatatgtag ctagtattta gtttacatat
     4021 agaattaggg tggtaacgcg atttataact cgtccctttg gtttaaaggg ggcgagtttt
     4081 ttagtattta aatttatgtg aaagaataaa aaattttgga attataatat ttataggaaa
     4141 gggtgattta catggcgaaa tttaagccat tggtagacag ttctgtaaaa caagcagaag
     4201 cacaagtttt tgattattgg aaagacataa atatacttga aaaaacttta gaaaaaggga
     4261 aagatgaccc aagttttgta ttctatgaag gacctccaac agctaatgga aatcctggag
     4321 tacaccatgt tttatctaga acacttaaag attcagtttg tagatataaa acaatgtctg
     4381 gttatcaagt aaagagaaaa gcaggttggg atacacatgg attaccagtt gaaatacaag
     4441 tagagaaaga gttaggtctt actagcaagc aacaaataga agagtatgga atagctgaat
     4501 ttaaccaaaa atgtagagag tcagttttct catttgaaaa acaatggaga ataatgactg
     4561 aaagaatggc ttatgaagtt gatttagata atccatatat aactttagat aataactata
     4621 tagagtctgt ctggtggata ctgaataaat tcaacaaaga aggatatatc tatgaagggc
     4681 ataaaatact tccttattgc ccaagatgtg gaactggtct tgctagtcat gaggttgcac
     4741 aaggatataa agaagtaaaa actaatacag ttatagctaa atttaagaaa aaagatgctg
     4801 atgaatactt cttagcatgg acaacaactc catggactct accatcaaat gtttcgttaa
     4861 cagtaaatgc tgatgtagat tatttaaaag ttaaaaaagg tgatgaggta tattatgtat
     4921 ctaaaccttt agctaataaa gtattaggtg aagactatga agttttagaa gaaatgaaag
     4981 gtaaagacct agaaggttta gaatatgaac aattaatgcc ttttgtagaa gttgacaaaa
     5041 aagcattttt tattacagta ggagattatg taactactga agatggtact ggtatagttc
     5101 atacagcacc agcatttggt gaagatgact acaacacagg taaaagatat gatttaccag
     5161 ttattcaacc agtagatgaa actggtaaat ttacatctac tccttgggaa ggtagatttg
     5221 taatggaaga tgggctagat gttgaaataa taaaatggct agcttctgaa aataaattat
     5281 atagcaaaga aaaagtagtt cataattatc cacactgttg gagatgtcaa actccactag
     5341 tatactatgc aaaaccaagc tggtatatag agatgactaa acttaaagat aaattaatag
     5401 aaaataataa tggtgtaaaa tggttcccaa gttttgtagg agaaaagaga tttggaaact
     5461 ggttagaaaa tctaaatgac tgggcaatat caagaactag atattgggga actccacttc
     5521 ctatatggag atgtgaatgt ggtcatacag attcagttgg ttcaagagct gaattagctg
     5581 aaaaagctat agaagatgta aatccagaaa cagttgaact tcatagacca tatgtagatg
     5641 atatacactt aaaatgtgaa aagtgtggaa aaccaatgac aagagtaaca gaggttatag
     5701 actgttggtt tgatagtggg gctatgccat ttgctcaaca ccactatcca tttgaaaata
     5761 aggaaaactt tgaccaactg ttcccagctg attatatatc agaaggtata gaccagacta
     5821 gaggttggtt ctactcatta cttgcagtat cgacatttgt attaggaaaa gcaccatata
     5881 agagtgtttt agttccagac cttgtacttg ataaagatgg taagaaaatg agtaaatcaa
     5941 gaggaaacac agtaaatcca atggaattat ttgaccaata tggagcagat gcattaagat
     6001 ggtatttact atatgtttct ccaccatgga ctccaactaa atttgatatg gatggattaa
     6061 aagaaatcca aagtaaattt ataggtacta tgaagaatgt atataatttc tttactttat
     6121 atgcaaacac agatgatata aatccaactg aattttttgt agaatataaa gatagaccag
     6181 aacttgatag atggatatta tctaaattca ataatttaat gaaagatgta gaagaaaatt
     6241 tagctatatt tgagttaaat aaaactttaa gaatgataca agattttata aatgatgatt
     6301 tatctaactg gtatataaga cgttcaagaa gaagattctg ggcaactgaa ttaacagaag
     6361 ataaaaaagc agtgtacaat acaacttatg aattgttaca tggtctttgt agagctatag
     6421 ctccatttgc accatatatg tcagaagaaa tgtatagaaa cttaacagga gaagtatctg
     6481 ttcatttagc tgaatatcct aagtgtaatg aagagttagt agatactaaa ttagaagaaa
     6541 aaatggattt agctaaaaac ttagttactt taggtagagc ttcaagagaa gtagagagaa
     6601 taaaagttcg tcaaccatta caaaaagtgt tagtagatgg taagtttgaa gatacaataa
     6661 gtgatgtagt tgatttaata aaagaagagc ttaatgttaa agaggtaata tttgctaagg
     6721 atttagatga gtatatgaac tttagtttaa aaccaaactt taaggaagca ggaccattat
     6781 taggttctaa gatgaattta ttcgttggag cattaagtaa attaaatgct catgaaactg
     6841 ctaataaact tgaaaaaggt gagactttaa cagttgattt agatggtgaa gcttttgaat
     6901 ttaataagga tttagtatta ataggtataa ctgctaagga aggatttaat gtatctgttg
     6961 aaaataatct ttttgttata ctagatacta agctaactga agagttgatt aatgaaggtt
     7021 atgctagaga atttatatct aaagtacagc aattaagaaa atctaataac tttgaagtat
     7081 tagataatat agtaatagaa tactgtggtg atgatgaaat tgctaaggct gttgaccatt
     7141 ttgatgaata catcaagtct gaaactttag ctttagaaat aaacagagta gatgataaat
     7201 ctttagagga acaaaactta aatgaccata tgacaggtat aaaagttata aaaaaataaa
     7261 aagaatagtt agttaaccaa aatccctata gaaattagtt tatagatttc tatagggatt
     7321 ttttatgtaa tataattatc tattcaataa aatgttttaa tattccaaaa tttacaataa
     7381 ataaatctat ataaatcata taaattattt atgtcatgtg atagaatatt aatgaataaa
     7441 cgtttaattg aggatgaata atatttttag taagctactt ctgataaatt tatttataaa
     7501 tttatcgtgt gttctgcagg gtggtttatt tattatgcaa aggggtgcgt tttgatgtat
     7561 ggtgaaagta aaaagctaag tattgataat aacatatttt tacaatattg tcttattata
     7621 ttgggtatga ttctatcaac cttaggaata aatttatatc tagcaccagc caagttatta
     7681 agtggaggag tagctggaat ttgtgttata ttatacaaat tatttggtat aaatcaggga
     7741 atatcaagtt tcttgatgaa cataccaata tttattatag ctagaaagta ttttgataat
     7801 aaatttttgc taataagttt tgtcaatatg ttactatttt ctctagcact ggggctaaca
     7861 caagatattg ccaaatattt tccaatagat gatactatgc tacagtgtat ttatggtggt
     7921 gcattaactg gtataggtat ggggttaact tttaaagcaa gagctactgc tggaggactt
     7981 gatataatag cagccataat gaaaagaaag tatgatatac ctatgaaaaa tacattttta
     8041 tttattaatt tttttgtagt atgtgcagga gctttcttat ttggagctaa attggtgatg
     8101 tacactttaa ttacaatgta tataatttct tttactatgg atataggaaa agattgcttt
     8161 gatagaaaaa aatccatact tttaatatca aataaatatg atgaaatatc caaagtgata
     8221 atgaataaaa tgggaagagg agtcactttt ttggaggcag aaggtgcata tacacaaaat
     8281 aagaaaaaaa tgatttattg tataatatct gcaaacgaaa taggtaaatt taaagattta
     8341 atttacgata tggatagtga agcttttatt tctgtcaata acgttgaaga agtaaaaggt
     8401 ggaggcttta aggataaatt tctttaagat tgtatatgtt aaaaaatacc atatgtattg
     8461 agattatacc tagttagatg tatactgtag ttaatggggt acatgaagta ctcaataata
     8521 tactaggagg tataagaatt atgttaagag agagatttgt agtaagaaat catcataatc
     8581 cacatacaag tggatgttgt gggactggag gagaaggatg ttgtggagga gataaccatg
     8641 aacattcaca tcaacattct cacagtcatg ggcatgaaca tgagcatgta aatggtgtag
     8701 gtcacgacca taatcatgat gatggttttt caggtactca tgaacatagc catgagcata
     8761 cacatacaca tggtcatgaa cattcagatg ggcatacaca tgaacataaa taaaaacatt
     8821 ttaaatttat aagagatttt tattttgtga ttccatgaag gtttaaataa aaattgaata
     8881 ttttaagtaa aaattattga caataaataa gaaagtgaac tgttaaagta gataacttaa
     8941 atagttcact tttattaatt atctaagtat taaatttaaa tccaatgatt aactaaaaaa
     9001 gaagctatat tttcataagg tattttttct gtttttaata aactttggac aactaaacca
     9061 tcaactattg acatgagtaa aaaagaaaat gattcagcat cctgttcatt ttcgctacta
     9121 ttatattttt ctagtgattc aacaatttca gatttaagag tagttctcca ttcaatatat
     9181 tttaatctca ttctttcttt taaatcatta tttgaagtaa ttgcctcaca aataagatac
     9241 atatgtaccc taccacgact ttctatagta gatattttct ctaagattaa gttaaccatt
     9301 tctatttgag aattttttga tttgacattt tttacacagt ttagtatagc actagttata
     9361 acttctaaat gagtatccgc aatatcatat ataatatcat ttttagatgc gtagtgataa
     9421 tacaaagtac ctttacttat atttacatct tttgcaatat cagcaaggct agtattttga
     9481 actccatttt tggctattaa attggttgca gatgataaaa tagcctgttt catattattt
     9541 gttttcataa aacctacctt tccatgtata tttataacaa attaataaca ttataagctt
     9601 atgaatattt attgtcaata aagcgagaat ctatatctat tgactttttt tcattcaata
     9661 tataaaataa taattaaatc ggtcgtacga ccgactgaat aaataaaata atatcttata
     9721 gtgaggttaa tatggataaa ataaaattga tagtggacag tgcatgtgac ttacctgatg
     9781 atataataga aaaatacaac atagaagttg ttggtttaaa cgtatctttt ggagaagaaa
     9841 gttatatatc tggaaaggaa atagataatg aaactttcta tagaaaaatg agtgagagta
     9901 aagtgctacc aaaaacatca tgtccttctc ctgataaatt tttagaatca taccattgtc
     9961 aggaagaaag tgtattggtg attaatataa gttcaggatt atctgggact tataatagtg
    10021 cacttttagc aaaagatatg tttgaaaagg aaggaaataa caagaaagtg gaggttatag
    10081 attcacttag tgggtcaatt ggaataggac aattagtgtt aaaagcagca atattaataa
    10141 aagaaggaaa agaaattgaa gagattgttt caataataaa taaatataaa gataatcctc
    10201 cattttttgg aacacttgaa actttagaaa atgctattaa gggtggtaga ataaatccaa
    10261 tagcaggaaa aattataaat accttgaatt ttaaggcgat aatacaagtt gcggatggcg
    10321 ttgtaactcc tatagataag gcaagaggcg aaggaaacag tttgaaaaag ttgattactt
    10381 tggtagagtc aaaaattaaa gataaagaag aaaaggtttt atttatagga catgctaatt
    10441 gcccagaaaa ggctcaaaag gtgagggaag ttatggaaaa ggatgtaaaa tacaaagatg
    10501 ttgtaatctg tgaagttggt tcagtgatgg ggacatatac ttcaaagggt gcaatcttaa
    10561 taactgcaat ataaataatg aattgaaata aatcatagat tgagaaatta aagccatata
    10621 aaaatgctta aaacattttt atatggtttt ttaattttat caaattgatt ttagaattaa
    10681 ccaactacac atatttcttt gtcatgtttg tttaatatct cacaaccatc ttcagtaaca
    10741 agtactaaat cttctattct aactccaaac tcacctggta aatatatacc tggctctata
    10801 gagaatatca ttcctggttg tacagtatct gtatttatag aagatacatc tcctttatca
    10861 tgaacttcaa gtcctataga gtgaccagtt ctatgagtga agtactgtcc atatcccttt
    10921 tcagttatgt agtcacgagc agcagcatct atatcacaga atctaacacc tggttttaca
    10981 atagcttcag ctctcttatt agcctcaaga actatttcaa atatttctct acctttttca
    11041 ggtatttctt tatagaatac agttcttgtc atatcagcac aatagttatc ttttatacaa
    11101 cctatatcta atattattgc atctcctggt ttcacaagtg catttccagg ttcaccatgt
    11161 ggattagctc cattaggtcc aaaacctata atagggtcaa atgaaaatcc atcagtacct
    11221 aggtcttcat atattttgga taatttacca acaagttgtt tctctgtaag ttcaccagaa
    11281 acagtagctt taagttgttc catagccttg tcatttaatt ttgaagctat tctcattaac
    11341 tctttttctt cttcatcttt acacattctt aaagtatcta ttatataaga tgaatttaca
    11401 aacttacttc ctccacctaa ttctattaag tttaataaga atctagctgg ccagttttta
    11461 tcaacaccca tagtagcatt tttgtctata tgttcagtta ttatttctac tggattttga
    11521 gtatcattaa accaaaccat ttcaacacct aaatcttctg aaactgggaa taattcattt
    11581 ataaataatt tattattgcc atttaaattt aagtataatg ctaataatct ttcacctgga
    11641 tggattagta ctcctgtaag ataaaatata gaagtagggt cacttactaa catttgagaa
    11701 atatcatctt ttttcatttg ttcaagtaca gcatttaatc tttgagcttt cataataatt
    11761 tcctcctaat caaaattcaa tacaaatcaa ttttagcatt attaaacaaa aaagagtata
    11821 cttttcttct agaaatataa aaaaaatttg ttatatattt tttatattaa gtttaatctc
    11881 tacttttgca ttaaatacgg aagtaaaatg actttttatt gtgaaaa
//
LOCUS       DAEMAE010000112        11893 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEMAE010000112 DAEMAE010000000
VERSION     DAEMAE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.114"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            255..719
                     /locus_tag="KPH93_002339"
     CDS             255..719
                     /locus_tag="KPH93_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG1689149.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            726..1220
                     /locus_tag="KPH93_002340"
     CDS             726..1220
                     /locus_tag="KPH93_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG1689150.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1236..2261
                     /locus_tag="KPH93_002341"
     CDS             1236..2261
                     /locus_tag="KPH93_002341"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG1689151.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLKAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2248..4695
                     /locus_tag="KPH93_002342"
     CDS             2248..4695
                     /locus_tag="KPH93_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG1689152.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4875..6248
                     /gene="radA"
                     /locus_tag="KPH93_002343"
     CDS             4875..6248
                     /gene="radA"
                     /locus_tag="KPH93_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG1689153.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEMWPVDNLRQAIN
                     IVLGRNQ"
     gene            6251..7321
                     /gene="disA"
                     /locus_tag="KPH93_002344"
     CDS             6251..7321
                     /gene="disA"
                     /locus_tag="KPH93_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG1689154.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAITNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7410..8504
                     /locus_tag="KPH93_002345"
     CDS             7410..8504
                     /locus_tag="KPH93_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG1689155.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8633..10810
                     /locus_tag="KPH93_002346"
     CDS             8633..10810
                     /locus_tag="KPH93_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689156.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRDIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKAYESMKDNIEKNIDVYLKNIFSIVGEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSVIVFSLLILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGAFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILALMINQIRYFRQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLIALKE"
     gene            complement(10996..11841)
                     /locus_tag="KPH93_002347"
     CDS             complement(10996..11841)
                     /locus_tag="KPH93_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689157.1"
                     /translation="MNRNNFSHEIVEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
BASE COUNT         4918 a         1226 c         2135 g         3614 t
ORIGIN      
        1 caataaagat gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca
       61 tgaagcaaaa aattagcact tggttatcaa taaatataat aaataaaaac tcataattaa
      121 tgaaagaata aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc
      181 tttaaaaatt taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt
      241 gtgtggtgat attaatggca actatgacag atataattga aaaatttatt aaagatttga
      301 tggaagaaga taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg
      361 caccatcaca aataaattat gtcttaacaa caagatttac aatagataga ggatattaca
      421 ttgaaagtaa aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag
      481 atggacatat tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag
      541 caaaggaatt attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat
      601 taatattata tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga
      661 aggtaaggtc taatatttta aagaatataa taataggatt atttagtata gaggagtgat
      721 aatttatgct atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag
      781 tcaatggaga aaaaacagag atgtatttat gtagtgagtg cgcaaaagag aatacagaaa
      841 tgaattttaa tttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc
      901 aacctaaaaa agaacttgag gaaaagctag tttgtcctaa atgtaataca acatatagtg
      961 agtttaagaa taatggaaga tttggatgta gtgaatgtta taatgcattt agtagtcaag
     1021 taaatccaat gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa
     1081 aatcttttta taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata
     1141 gagctataaa aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt
     1201 taaaaggttc tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat
     1261 caagagttag gctagctaga aatttaaata actatccctt tccaaataaa ttagataaag
     1321 aatgtgccat ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac
     1381 aaaaagaaga atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta
     1441 tggttgaaga acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag
     1501 taaaaaaaga taaaactata agcataatga taaatgaaga agaccatata aggatacaaa
     1561 ctatatgtga tgacttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt
     1621 tgttagaatc aagtttagaa tatgcattta acacaaagct aggttatcta acatcttgtc
     1681 caactaacac aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc
     1741 agctaggtta tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag
     1801 ggatatatgg agaacgaaca gaagcattag gaaatatata tcaaatatct aatcaactta
     1861 cacttggaag aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa
     1921 tttcaaaaga aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag
     1981 ataaaatatt tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg
     2041 caatgagcca tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag
     2101 atttaaaggc tatagaacaa cttatgatag gtatacaacc agcacatcag tcaataatgt
     2161 ataagagtga tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt
     2221 tggaaaaatt aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa
     2281 aaggcaattg atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt
     2341 gaacatatac ttttaggtct tttaagagaa gaagaaggta tagccgctaa agttctaagt
     2401 aaagttggat ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga
     2461 gaagaaatct cagaagacat agtattaagt ccaaggagta aacaaatact tgaattatca
     2521 ggtatgtttg caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca
     2581 attattcaag aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat
     2641 aggactttag cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa
     2701 gcagaaaata gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat
     2761 ggaagaaatc ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa
     2821 aaagaaatac aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg
     2881 ataggagacc caggcgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc
     2941 ttaggaaatg ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca
     3001 ttattggctg gggctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat
     3061 gaagttgtta aaaatggaaa cataatctta tttatagatg agatgcatac gataataggg
     3121 gctgggtcta ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca
     3181 agaggagaaa tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa
     3241 aaagattctg ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag
     3301 gactcaataa aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa
     3361 ataactgatg atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga
     3421 tatcttccag ataaagcaat agacttgatt gatgaagctg catcaaaagt aagattaaaa
     3481 gaaaatacac ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa
     3541 gaaaaagaag aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa
     3601 caaggtctac ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa
     3661 cattcagatt tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata
     3721 ccagttaata aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg
     3781 cacaatagag ttataggtca agaacaagca gtaaaatcta tctcaaaggc aatcagaagg
     3841 tcaagagcag gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca
     3901 acaggagtag gtaaaacaga gttatctaag gcattagcag aagtacaatt tggagatgaa
     3961 aatcaaataa ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg
     4021 ataggttcac ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta
     4081 agaagaaatc catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta
     4141 tttaatattc tattacaaat tctagatgat ggtagactga ctgattcaaa aggaagaact
     4201 gtagatttta agaatactat tgtaataatg acatcaaacg ttggagcatc tacaattggt
     4261 agacaaaaaa ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat
     4321 gaaaaaatga aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta
     4381 aacagaatag atgatataat tgttttccac tcattaaatg aaaatcatat atcaaaaata
     4441 gtacttttaa tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa
     4501 atgagtgatg aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca
     4561 agacctctta aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta
     4621 aaaggtaatg tgaaaaaggg aagtaatgta gtagcaaaag taaaagatga aaaaatagtt
     4681 tttgaaacta aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata
     4741 atctaaacaa aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta
     4801 tttaatcttt acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta
     4861 aggatgataa aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgtggatatg
     4921 aaacagctaa gtggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag
     4981 aaatagacca aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac
     5041 cagtcagtat aaactcaata gaaagtaaag aagaagagag gtttacaaca gatataaacg
     5101 aattagatag agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg
     5161 accctggtat aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag
     5221 gaaaaactgt tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta
     5281 aaagattagg tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa
     5341 ttgaatctta tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg
     5401 tcttcagtcc agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt
     5461 caaaattcat gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta
     5521 caaaagaagg ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat
     5581 attttgaagg agaaagatat aatacttata gattagtgag agctgttaaa aatcgatttg
     5641 gctcaactaa tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgaca
     5701 atccatctaa aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat
     5761 ctactgtaga aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa
     5821 gttttggtat accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat
     5881 tagcagtatt agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca
     5941 tagttggagg aataaagata aatgaacctt caattgattt agggatagcc atttctgtag
     6001 catctagttt tagaaatata cctatagatg aagatatagc tgttacagga gaagtgggac
     6061 ttacaggaga agtcagagca gtaagcttta tagaaaagag aatagcagag tgtaaaaaac
     6121 ttggatttaa aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag
     6181 gcatagaaat gtggccagta gataacttaa gacaagctat aaatatagta cttgggagga
     6241 atcaataaat atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat
     6301 atctcctgga actccactta gattaggttt aaacaatgta ctaagagcta agactggtgg
     6361 actaattgta attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat
     6421 aaatgcagaa tattcaccat catatctata tgaattagct aaaatggatg gagctatagt
     6481 tttaagtggt gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt
     6541 tatagaaaca tcagagacgg gaacaagaca tagaacagca gaaagagtag caaaacaaac
     6601 tggtgctata gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa
     6661 tgagaagtat gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca
     6721 aactctggaa aaatataaga cagtattgga ccaagctata acaaatttaa atgccttaga
     6781 gtttaatgat ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt
     6841 aatgagagtt acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt
     6901 agtaagtatg caattagaag aattaatggg tacgaccaga atagaccaga aattaatatt
     6961 caaagattat aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt
     7021 aaattcagaa gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt
     7081 ttcagaaagt atggatatgc ctataaaaac aagaggttat aggattctta gtaaaataca
     7141 tagactacca acagccataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat
     7201 tttagatgca tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac
     7261 atatataaaa aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg
     7321 atgaatttta aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata
     7381 atagataagt ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta
     7441 ttttacttgg atttacaata ggtataacaa cctatttaac attaatgaaa gactttgaaa
     7501 tattaacatt tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa
     7561 tagccatttt aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta
     7621 aaatagtgga taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg
     7681 gtcttatagt aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa
     7741 tagtaggtgg aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag
     7801 ttgcgcttaa aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc
     7861 ctattaagga taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa
     7921 aagtattaga tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt
     7981 ttatagaagg aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag
     8041 attcttcaga tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa
     8101 ttcagaaaga attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag
     8161 aagttgatag taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg
     8221 attataactt aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt
     8281 tagctaatgc tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta
     8341 aagaaggaaa agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg
     8401 ttgatggagg aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc
     8461 aaactcctgc aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc
     8521 tataatttgg gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat
     8581 gtttaatgta ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa
     8641 aaagattata tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag
     8701 ttttgcagat gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag
     8761 tatgagtaat atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat
     8821 tggcttgatg aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat
     8881 gggtgctggt ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag
     8941 taaggataga gatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa
     9001 tttaactata aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt
     9061 agggtcatta ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggaaattc
     9121 agatataatt gaaaatggcc aacttataaa aaacagaaac ttatgtctaa ctgcaatgga
     9181 tgaatatgga agaataccta atggaaatgt agatactata aataaaaaag acttatctat
     9241 gccttatgga atatcaactg attatgataa attgattgta gaaaccaaag aagcttacaa
     9301 aaataatgat gtggtttttg tggagcttgg agatacatat agattagatt tatataaacc
     9361 aaatttaaat gaaaaagcat atgagagtat gaaagataac atagaaaaaa atatagatgt
     9421 atatttaaaa aatatatttt ctattgttgg agaaaatgac actgtctaca tagcaagtgc
     9481 atttccaagt gatttagatt ataaaaataa aagaagactc tctccaataa taaaattaaa
     9541 tggagaaggg aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa
     9601 tttagatgtt ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg
     9661 aagaaaatat gaacttatca acagagatga taataaagaa tttttgatgg atgaatatca
     9721 aaagatagta tctatttcaa gcataaggtc tacaatactg aatggttttg taagcatagt
     9781 tttcttgtca tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa
     9841 ttataaagaa acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc
     9901 attgtcattt atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg
     9961 aataattata ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt
    10021 aaaaaacatg ctgtttttta ctggattaac catagtaatt atggttattg atgctggatt
    10081 tggctcatat ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata
    10141 ttatggagta ggaaatgagt atcaaggtgt ggcaattggt agtgcaatat ttacatttgc
    10201 aattttactt acatataaaa atataccaaa gtggtctgta atagtttttt ctttgcttat
    10261 cttaattaca agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg
    10321 tgttgcattt ttactattta tactattaat atataatgtg aagatagact tcaaaaaaat
    10381 tgtattatta ggtatagcag tattatttgt attaggagct tttgtagcaa tagatatgat
    10441 tttgggaagt aattcacatt taggtatgtt tgtaaaagaa atttacttta atggaccagg
    10501 agaaataata caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag
    10561 tgcatgggta aatatactgt taactggaat aggggtgatt ttagctctta tgattaatca
    10621 gattagatac tttaggcaac tgatggatga gtatccaatt gtatttaaag gatttatagc
    10681 atcaatagca ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc
    10741 tacagccttt atttatgtaa ttgtacctat gataacatta agtataaatc tcatagcatt
    10801 aaaagaataa gtgctgtatg aatactatgt aaatatgaac acattaattt aacttccttg
    10861 tttacaaaat aaagaagaaa gtttataaaa gataaattat aataaaaaag agtttatctt
    10921 aaaattgatt tttctaatca aattttaagg taaacttttt ttattataaa gataaataac
    10981 atataatctc atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt
    11041 ttctttaaaa attctgtaaa ataatttcgt attagtaaat ccatgagctt ctaatatact
    11101 gcataaggta tcatttgtgt taattaagtc tttataaata tgagaaatac gcacatcatt
    11161 taaatactct aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg
    11221 ttgtaagtgt gctaccttag cgattttgtt aatagaaata ggcattttat aattaatctt
    11281 tgtatattct aaaactggct caaattttga taaatcaagt actgtttttt tcatattttt
    11341 attacctaca gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct
    11401 tgtaaagagc agattagctg caggaggaga actaagttta atttctctca tgttttctaa
    11461 tataagttta actttacttg aatttttttt atagttattg tcattggcat taaccttaaa
    11521 gtcaaaatag caatttttaa aatcaggtat atatttattt agaaaagaca atggtacttg
    11581 caataatata gaggtatttt gataagtaca tctagttgag tgaataattc cagaattaat
    11641 gaacatacac tcgttagctt ttaaattata ggtgtaacct tccatgttaa tttgtaaatc
    11701 gccagaagtt atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc
    11761 actatcatca aatagtttaa aattaacatc taaattatca ataggttcaa ctatttcatg
    11821 tgaaaaatta tttctgttca taataatctc ctccttataa ttttaaatta taaatagtaa
    11881 atttataata ttc
//
LOCUS       DAEMAE010000113        11737 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEMAE010000113 DAEMAE010000000
VERSION     DAEMAE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.115"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            194..1075
                     /locus_tag="KPH93_002348"
     CDS             194..1075
                     /locus_tag="KPH93_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1689158.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCIEAEKIMIDINYELSKLSNTDEYPYALSISYGLVEWDGISDIETLIAE
                     ADSLMYENKLKNKSTLN"
     gene            1123..3207
                     /locus_tag="KPH93_002349"
     CDS             1123..3207
                     /locus_tag="KPH93_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1689159.1"
                     /translation="MSKYIDFANIEYSLLMNALDVSVSKHLLDEHFTVVWSNDRYYEM
                     FGYTKEEYETLFHNQCDLFYKDNPEDWNELVEYVEKTFSTGAKKYDYVCRMKHRNGKK
                     LWIKLFGFLTGEVVNGYPISYSVMIDITEQMMIKIEQTVTYNNFPGLIAKFKVTDFGF
                     QLLDANKKYFDMFKDNVNFLLDDINLESYLSAVYKYQSDFKAGKYVCFSVSPQNINGD
                     IVHMQVVAECIDWEDNKPIYLLIYSDVTKLVKQNEIIEKNNKELKKLAFIDSVTGGIN
                     RTKFNIIAKEIISKSPSGTYTLIWLNMKKFKLINDLAGNIAGDNLLKYIYNCISKHLH
                     EGELVTRIFADKFILLLKDKPEDITSRLNSMVTHINRFNNNADYKYILYFTAGAYVIY
                     DTSLDITNIQDSANVASKSIKNVENHGLCICNFYSKKDKEKLILEQSIENRMYEALEK
                     REFQVYLQPKLELSTQTICGAEALVRWIHPVEGIIPPNEFIPLFEKNGFIIQLDLYVF
                     EEVCKTLKKWIDDGLNPINISVNMSRYHFSSNNFIKTYDKIAKKYGIPSHLIEIEITE
                     TIVFEDVKVFSEIINEIHKNGFKCSIDDFGSGYSSINMLKDIYVDTLKLDRAFFQSEH
                     LDSQRERDIIVSIIELAKKLKMTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIK
                     DFEKLMFQNSSN"
     gene            complement(3310..3474)
                     /locus_tag="KPH93_002350"
     CDS             complement(3310..3474)
                     /locus_tag="KPH93_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689160.1"
                     /translation="MNKKAILALKLSTFVIALAEVVILKVLRFNHTTNTHQTAVRKTI
                     AWWGFSYAKK"
     gene            complement(3467..6034)
                     /locus_tag="KPH93_002351"
     CDS             complement(3467..6034)
                     /locus_tag="KPH93_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG1689161.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGISVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDISKKIGVLFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDEVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIQGGAISHLDD
                     IYWLTKSELEALIEQLDKNRSLSDMNGLIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     ALQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(6037..6642)
                     /locus_tag="KPH93_002352"
     CDS             complement(6037..6642)
                     /locus_tag="KPH93_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689162.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(7027..8487)
                     /locus_tag="KPH93_002353"
     CDS             complement(7027..8487)
                     /locus_tag="KPH93_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG1689163.1"
                     /translation="MFNELRTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEAIKNTKESIKVWADMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8788..9366
                     /locus_tag="KPH93_002354"
     CDS             8788..9366
                     /locus_tag="KPH93_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689164.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILGNMPQKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYNNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            9417..9650
                     /locus_tag="KPH93_002355"
     CDS             9417..9650
                     /locus_tag="KPH93_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689165.1"
                     /translation="MIELIQKLNDVIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFVIILGIKVRKKVLIVIAIIAIIVNVLIILDR"
     gene            9659..10909
                     /locus_tag="KPH93_002356"
     CDS             9659..10909
                     /locus_tag="KPH93_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1689166.1"
                     /translation="MVKSLNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIIFSPIGGAIADIFNRRNLMVIFDFSSSITVLILALFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIAISCVSFFLSAIMEIFIQIPFIKQEQTSNIIKTIFTDIRDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKITNLHIPFIICALVFIPMAFSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFSGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNEKL"
     gene            11070..11639
                     /locus_tag="KPH93_002357"
     CDS             11070..11639
                     /locus_tag="KPH93_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG1689167.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3965 a         1922 c         1788 g         4062 t
ORIGIN      
        1 ttttattatt tgtacttatt taaggttaca tctagttgta aattggacaa tttttatgtt
       61 aattttattt atgcaaatta aagtaactta caaactttcc acaataaaaa accttttcta
      121 ttcgttaaac tgctccatca ttggtttagc aataaatata ttttttaggt ctttaatgtc
      181 aatagtgtta aatatgccag catatttatt tgatagtaat tctattacaa atatttacag
      241 agtattttct atattgctta gttttatagt tacaggatta tatttcagtt ataaaaatca
      301 taaaaataat aagattaact ttgaggcagt tttagaggac aaaaaaaact taattttttt
      361 attgagtctt atgtttggaa tgtacttgta tctatgtttg agtttgtcaa tatactatac
      421 tgttggaaat gtattgattt taaaattata tggaatcaag tctgccataa tagttttggt
      481 aggtaactat attgcagaaa gatatagctt tagaattact caaataaaag gttatgatat
      541 taaaaatcaa atagaatatg tagaaagtat aaagacagaa tatgaaatta ataaattgaa
      601 tgatattgta tatacagatt tactgacagg cttctacaat agaccttttg taaatcaaaa
      661 actatcagag tggacatcaa atcactacaa atttacctta tgttttactg atttaaacag
      721 cttaaaacct gtaaatgata aatttggaca ccaatttggt gataaataca tatccactac
      781 tgcacaaata ataaaaaaac ttataaatgt agaaaattcc ttactgttta gatatggtgg
      841 agatgaattt ttattccttt tacagaatac aagttgtata gaagcagaaa aaataatgat
      901 tgatataaat tatgaattat caaaattaag taataccgac gagtatcctt atgctctttc
      961 cataagctat ggattagttg agtgggatgg aatatccgat attgagactt taatagcaga
     1021 ggcagattct ttgatgtatg aaaataagct taaaaataaa tctactttaa actagctagt
     1081 tcagatattc tacaaaatat atgttataag ggagtactaa gaatgtcaaa atatatagat
     1141 tttgcaaata ttgaatacag tttattaatg aatgcactgg atgtaagtgt tagtaaacac
     1201 ctattagatg aacactttac tgttgtttgg tctaatgacc gttattatga aatgttcggc
     1261 tatacaaaag aagaatacga aactctattt cacaatcaat gcgacctctt ttacaaggat
     1321 aatccagagg attggaatga acttgtagag tatgtagaaa agacattctc caccggagca
     1381 aaaaaatatg actatgtctg tcgtatgaaa caccgtaatg gaaaaaaatt atggataaag
     1441 ttatttggat ttttaacagg agaagtcgtt aatggatatc ctatttcata ctctgttatg
     1501 attgatatta cagaacaaat gatgattaaa atagaacaaa ctgtaactta caacaatttt
     1561 ccaggtctca ttgccaagtt taaggttact gactttggat tccaacttct tgatgctaat
     1621 aaaaaatact ttgacatgtt caaagataat gtaaattttc tattagatga tataaattta
     1681 gaaagttatc ttagtgctgt gtacaaatat caaagtgatt ttaaagctgg gaaatacgtt
     1741 tgtttttctg tttctccaca aaatatcaat ggggatattg ttcatatgca agttgtagct
     1801 gaatgtatag attgggaaga taataagcct atataccttt taatatattc agatgtcacg
     1861 aaattagtaa aacaaaacga gattattgaa aaaaacaata aagaattaaa gaaattagct
     1921 tttattgact cagttactgg tggtataaat cgtacaaagt ttaacataat agcaaaagaa
     1981 attattagta aatcaccatc gggtacatac actctaatat ggctgaatat gaaaaaattt
     2041 aaactaataa atgatttagc aggaaatata gctggagata atcttttaaa atatatctac
     2101 aactgtataa gtaaacactt acatgagggt gaattggtga ccagaatctt tgctgataaa
     2161 ttcattcttc ttttaaaaga taaaccagaa gatataactt ctagactgaa ctcaatggtt
     2221 acacatatta atagattcaa caataacgca gattataaat atatacttta ttttactgct
     2281 ggtgcatatg taatttatga tacttcttta gatataacaa atatccaaga tagtgcaaat
     2341 gtagcttcta aatcaattaa aaacgtagaa aatcacggtt tatgtatttg taatttctat
     2401 agtaaaaaag ataaggaaaa acttattcta gaacaaagca tagaaaatag aatgtatgaa
     2461 gcacttgaaa aaagagaatt tcaagtttat ttgcaaccca agttggagtt atctacccaa
     2521 accatatgtg gtgcagaagc tttagtaaga tggattcatc ctgttgaagg aataattcct
     2581 ccaaacgaat ttattccatt gtttgagaaa aatggattta ttatacagtt ggatttatat
     2641 gtttttgagg aagtttgcaa gactttaaaa aaatggattg atgatggatt aaatcctata
     2701 aatatttcag taaatatgtc tcgttatcat ttttcatcca ataatttcat caagacatat
     2761 gataaaatag ctaaaaaata tggtattcct tctcacctta tagaaattga aattacagaa
     2821 acaattgtat ttgaagatgt caaagtattt agtgaaataa taaatgaaat tcataaaaat
     2881 ggctttaaat gctctattga tgattttgga agtggctatt cttcaatcaa tatgcttaag
     2941 gatatttatg tagatacatt aaaattagat agagcatttt ttcaatcaga acatttagat
     3001 tctcaacgtg aaagagatat aattgtatct ataatcgaac tggctaaaaa gttaaaaatg
     3061 accactgtag ctgagggaat agagaccagt tttcaaaaag atttcttgca agaaataaac
     3121 tgtgacatgt tacaaggata cgtattctct agacctttac caataaaaga ttttgaaaaa
     3181 cttatgtttc aaaactcctc caattaacaa tataaaattg cttattataa gtgatattat
     3241 tgtgtcaaca aataaaggaa ttgtctcaaa attgattttt tctaatcaaa tttttatttt
     3301 gatacattct catttttttg cataagaaaa tccccaccaa gctatggttt ttcttaccgc
     3361 tgtttggtgg gtattagtag tatgattaaa tctcaaaact ttaagtatta ctacttccgc
     3421 taacgcaatc acaaaggttg aaagcttcaa agctaaaata gcctttttat tcatcaaacg
     3481 tcaccgtacc tgccaacccg tccaccgtta tcatttgtcc gctcttaata gttcgtgtgg
     3541 caatgtgtgt agccataacg gctgggatgc catattcacg tgcgacaatg cttgaatggc
     3601 taagaggacc accaatgtcc gttacaatcg cacttgcaga tgaaaataat ggtgtccatg
     3661 ctggagttgt ggtaactgca accaaaacac ttccaggttg aaagctttca aaatctgctg
     3721 gactattcag tacacaagct ggagccgtca caacaccagt actggtacca atgcccgtca
     3781 acacaatttt cccatctttc tgcttctgta gtgcttgtga tatagttttt ttgtttttct
     3841 ctggcaactt ggatggtgac acataaccca tatatttttt aagttcagct tttcgaacag
     3901 gtattaaccc gttcatatca gatagagatc tatttttatc caactgctct ataagtgcct
     3961 ccaattccga ttttgtgagc caataaatat catctaaatg tgaaatagct cctccttgta
     4021 tgaatcgttc tgagatttcc tgaaacatac agcgaatcag aggatgtccc attcccatca
     4081 aataaatagc attttcgcgc ataggagagg tttcctgcgc ccaatggagc aacttaaaaa
     4141 atagtttttt acgcaatcca cctatgtgtt gtaaaatctc ttcttctgca tgttttctac
     4201 gcttttcaaa cgtggtctgg cgtaaaaaag gactttcccc ttttccttcc acaaatgttt
     4261 ttacggattc aaaagttggt gtaagtgtct cctgtggtgt ggaatacgca aaatcaaatt
     4321 cataagcggt acgaccaaac tctttaaagt acttattgat tcgatttttc cattctatcc
     4381 atacttcctg tgaaactcca acaggcacaa ctgaagacat gaaatcttct gctatcttta
     4441 ttgcagaact gttttgcaga taaaggttga tgctgttatt ttgctttacc cattctgaaa
     4501 taccccacaa ctccttttcc gattgcagag caattgtatc aaacccaagc aggaaaacgg
     4561 aagtatccgt tataccagca cggcgaactg ctccttgaaa taattttgta aataatgttt
     4621 cactgataga agcagcaggc aacgtaagct gaatccttgt gaaataaata catgcggcat
     4681 aaaatattgt ctgaataccc tccataattt gatgggcgtt catcgtatgc aggggctttt
     4741 cttcccattg tttaatagca tcctcatatc cttttcgtgc cgtttcccag cgtgcaacgc
     4801 tgttcataag tgtcctacgt aaagagcgtg gggaaaggga tttgatagca atcaaaagtg
     4861 gctttgattt agcagaaaga taaacatatc cattaataac cgtgtatgcc ccgttttttg
     4921 gcagtagttt tttcgcactt ttaccaaaca tatctatcca taaaagctcc gatgcacggt
     4981 ttacgatttc aagaccaagc gtggcgaaca aaggtgtaac aggatttggt aagtgttctg
     5041 ccaaacttcc ctttgtgtat acaacatcct gttctggcaa tacccattcc ggtggcagaa
     5101 cagtaattgg acgagcctga acaatgtaca atttatcttt ttccagtgcc cactctacat
     5161 ccataggcat ttgataatac ttctcaattt tctttccaag ctgtatcagt tgcattacct
     5221 gattacgagt aagagcatat ttttttctca acctttcagg aaccatggtt tcttctgttc
     5281 cttctgaagt acgaaccgtc ataatctctt tatttgccac ttcatacgat ataattcgtt
     5341 cggagtcctt atccactacg attgtatcgg gtgtaactaa gctagaaacc accgcttcac
     5401 caagccccca tgcagcgtta ataatcattt cgctacgtct accattgatt gggtttaacg
     5461 taaacataat accagatgaa tcggaaaatg caagcttttg tactacaaca gcaagtgcga
     5521 caatctcgtg ctttatatta ttcttcacac gataagcaat agcacgagct gtccataagg
     5581 aagcccagca tctctttaca gcgtcaatga cttcatcctc accttgtatg ttaagatacg
     5641 tttcctgctg acctgcaaaa gaagcgtcag gtaaatcttc agcagtcgca gatgaacgga
     5701 cagctaccga tatgttccct aatccagcat aagctgtttt aattgcgtcc gacacttcct
     5761 gtggcatttc tccattatgg aaaagcaccc ctattttctt agatatatct tcaagttggc
     5821 tggtattatt ggaatcaata ccatctagta gtttattaat tcgagactga atatggttcg
     5881 tctcaacaaa aatctggtat gaagcagtcg taacatgaaa accgtctggc acagagatgc
     5941 ctgctgtcaa tagttttgat aaagacatcc cttttccgcc aacaatttca atggttgctt
     6001 gcttatgttc tagtggtaag acataataaa tcataatcat tcacctccat cattcataat
     6061 tccgtgcaga aatatattca ttgtttcatc caacattttt tccgaactat ctgatggtcg
     6121 ggacgtgtag ctgattgata acatggagtt aattaacaga cgtgtcacaa gagcggtatc
     6181 cgttttgcga aagatacctg tggtaacacc attatcaatt accgatttta taatatcttg
     6241 atatgcataa cgagcatctt taagtctctt tcgatactct tcttctaaat aatcagattc
     6301 tgtatttaac cacatcagta cagttctata ctgctctgga aatctaagat gattaagcat
     6361 gatttttcta aggcactgtt gtggcgaagg ttcaccatca ataatcctat gaacttgttg
     6421 gatagttttt tctatttctt tctcaactgc atatacaaca atctcgtctt tcgttttgaa
     6481 aaaatcgtat agacttgact ttcccatgtt ggctaaaact gcaatttctc tcattgatgt
     6541 tttttggaat ccattttcag ccatgagact caaagcaaca gcaactattt tttctcggcg
     6601 ttctgcttgt tgttcgggtg taagcacaat tccttttggc atagggcgat acctcctttt
     6661 agcgaccact ggtcgtttct tttttattta tcatacagcg accatcggtc acttgtcaag
     6721 gttaattttt ttcattgtat atggtactcg agataaaaat gttcatttta gaaattgctc
     6781 tatttaagta gaaataggct tcatcattag aaaacaagac tataaattta agatatttat
     6841 catgttcaaa tttcatcaat ctcaaagttt aagatattta ttaaaaaata attactaaga
     6901 aatagcatat aaaaattcaa aaaggagttg aacttaaaat tcaattacaa aaaatcaaat
     6961 ttaagacaac tcctttattt tttatttcta ttattcttta tctctactat tctttatctc
     7021 tatttattac aactctctaa catttaccac tactgcctta ggtctagtca tagcctcaat
     7081 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     7141 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     7201 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     7261 ttggttagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     7321 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     7381 atagaataaa ttattttttc taacattgcc tgttattaac tttgctcctt tatgtaatgc
     7441 atcatcaact aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     7501 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     7561 taatttatcc gctacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     7621 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7681 taatactata gccgcatctt ttccaccaag ttccaataat aatggcacca ttccagatat
     7741 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7801 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7861 atctggtata ccagcttttc tcataatttc taccacatga agtgcgctaa tagccccttg
     7921 tgtagcaggt tttaatacga ctgcatttcc accaataagt gctggagcaa tctttgacat
     7981 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggcactct
     8041 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatagcttc
     8101 accttccata ttttttccta catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     8161 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatttc
     8221 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttatatac
     8281 aggcatatca gcccatactt ttatactctc ttttgtattt tttatagcct catctacttc
     8341 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     8401 tattggtttt cttgaattag attctaccca ttttccattt attaaatttt tatatacatt
     8461 ttcttttgtt ctaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     8521 atttaaatta tagccattta ttcgttatgt aaacatttct ttttcttata aacgtttacc
     8581 tagaaaaatt ttttagagtt aacgtttttt ctacattttt ctattttaat aacatactcg
     8641 cttgaaactt attcaaatca aattttaata caagtctcat aatataaatc acttgacaaa
     8701 tttatgaata aacaatacta tatatctatg acttaaaaat ttttttaagt catagataac
     8761 acgtatatac agagaggatt gataaatatg gctaaaagct ttacagaaat agaaaaagat
     8821 aatataagaa aaagactaat ttcagaatgt gaaatcaact ggtctaaata tggatacaaa
     8881 aaaactaata ttgatgaact ttgttcaaaa tcaggaattg caaagggttc attctacaca
     8941 ttctttgatt caaaagaaaa gttatttttt gaagtactaa atggaatcca aaacagactt
     9001 atatcatcag tagatgaaat attaggtaac atgcctcaaa aagaaggttt tgcaaaagcc
     9061 ttaaagatgt tgtatcgatt atatgataaa aacccattct tatattcacc aaataatact
     9121 gattacatca ctttattgaa taagatacct aaagagatgc tttcacaact tgaatacaac
     9181 aatgtacaaa gtttttatag cttaattgaa agacataatc tcaagttaaa aatagagaaa
     9241 gaaaaagcct ttggtgtttg caatgcccta ctgtcaagcc tattgctaaa agaagatatt
     9301 ggatatgact acattgaagt atttgatttt atggtagaca atcttgtaga acacatactt
     9361 gagtaataat ttaaaataaa ttttctaaac atttctttat aactagaagg ggtaatatga
     9421 ttgaattaat acaaaaacta aatgatgtta tttacaaaat aaatgataaa ctaaatatcc
     9481 acacaaaact tgtagaccct agcagtgagc tcatgccgct ttactctatg ttatctataa
     9541 tcgtttctat atttgtaata atattaggta ttaaagtaag aaaaaaagtc cttatagtaa
     9601 ttgctattat agcaattatt gtaaatgttt taataatctt agataggtag gtatatatat
     9661 ggtaaaatct ttgaatactt ttaataaaaa tttcaacttg atgatattag gtcaaataat
     9721 ctcattattt ggtgcatcaa tacttaaatt tgctttgtct ttatacatac tcgatataac
     9781 tggtaaagca gaaatatttg ctactatact agcagtatct tcaatcccaa ttataatatt
     9841 ctcccctatt ggtggagcta ttgctgatat attcaacaga agaaacttaa tggttatttt
     9901 tgatttttca agcagtatta cagtgttaat cttagcactc tttttattca acaataatgg
     9961 ttcaatatta cttgtaggaa ttattatgac catattatca gtcataagca caatgtatca
    10021 accaactgtt cagtcaagta cacctctttt agttgataat gagcatttaa tgaatgcaaa
    10081 tggtattgta gctggagttg catcccttac aaatattgca ggacctgtat tgggaggtgt
    10141 attgtatggt gtgattggaa ttaatgcaat tatagctata agttgtgtaa gtttctttct
    10201 atctgcaatt atggagatat ttatacagat tccttttatc aaacaagagc aaaccagcaa
    10261 cattataaaa actatattta ctgatataag ggatggtatt gttcatatat gtaaagaaaa
    10321 tagatttata ctaaaaatac tttttttagc tatagcaata aacttatttt taagctctat
    10381 gttaatagtt ggtatcccct atatagtaaa aatcactatg ggagctagta acttcttata
    10441 tggtctagca gaaggagcaa tttcttttag ttcactagta gctgctgtga gtattggtat
    10501 tttctcaaaa tatctgaaaa tcactaattt acatatcccc tttatcatat gtgcacttgt
    10561 gtttatacca atggcatttt cagtataccc atttatatta aatacaggat ttccagttcc
    10621 gtttttaatc tttatagtat cttctatgct aatatttttt acaacgactc ttatctcaat
    10681 atatgttata acagtcacac aattaaaaac tccaaatgaa ctcttaggaa aagtaatgtc
    10741 aattcttttt tcaggaacag caattgcaat gccccttggt caggttatgt atggtaaact
    10801 ttttgattta ttatcagata gagtgtacat cattatacta ggagttggat ttattacatt
    10861 acttatagca tttgtatcaa agataacatt gaggaacgaa aagctttaaa tacaaaaaat
    10921 ttggacataa agatttaatt ttcactgatt tctgaattta aatacaaaat tgcgtatcag
    10981 tatgttgacg ttttggaaat tgattgatat aatcaatgga aaataaactt tataaaaatt
    11041 caaaatattc agctagagga gtgataaaaa tgagttacat gccaacttta gaacaagcat
    11101 gggaaatatt aagaaaatac aataaggaag aatttcatat aagacatgct caaattgtat
    11161 ctggagttat gagatactat gcaaaggagt acgaccctga aagagtagaa ttttgggaaa
    11221 tagttggact tttacatgat ttagattttg aaatgtatcc tgatgaacat tgtgttaaac
    11281 aaagagaatt aatgacagag cttgatttag ataaatcaat aatcgattct acaataagtc
    11341 atggatatgg attaactggt tcagatgtta aaccagaaca atttatggag aaagtattat
    11401 ttgctgtgga tgaacttact ggtttaattg gtgcagctgc aataatgcgt ccttcaaaga
    11461 gcgtttctga tttagaatta aaatctgtta agaagaaatt taaagataaa tcatttgctg
    11521 ctggctgttc tcgagacgtt ataagaagtg gtgcagaaat gcttggatgg gaacttgata
    11581 agcttatcca aacaactata gaagctatga gaagcttgat tccagagatg gcaatatagc
    11641 ttcctcatac atataccata gaagttacat ctttaaagag catttctttt tttgatgcaa
    11701 cttcttttta tattttgtag aataaaaagt ttaatgt
//
LOCUS       DAEMAE010000114        11724 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEMAE010000114 DAEMAE010000000
VERSION     DAEMAE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.116"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(225..1613)
                     /locus_tag="KPH93_002358"
     CDS             complement(225..1613)
                     /locus_tag="KPH93_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1689168.1"
                     /translation="MPVNSFDNYYMSWKPNKNRLSNPLYLSLAKCMEEDIEDYVLKEG
                     IKLPPQRELADFLDISLNTVTKAYNLCKEKGLLYGVAGSGTFVSQNAGLSKTILSEEK
                     INKNIDMAMLVPFYNHNKIIQSLAIEILQQPNADKYLEYTHPLGNCIQKQAAKKYLNK
                     FNVNAKEENIIIANGTQNALMLTLVSLFKPGDKIATDTYTYANFIGLANLLHIELVSI
                     NGDDKGMLPESLDNLCKIRDIHGVYLSPSCSNPTNITMRKNRREEIAHVIKKHDLILI
                     EDDIYAFILDNPLTPIYSVIPEQSVYIGGISKSICPGLRIAYMSFPDKFRKKLENGVY
                     SCNLKTSSLNVEIATQLILKDMYEDIICDKRRMAIRRNKIFKKYFPDLDEGYNENSYY
                     QWICLPKGITGRIFELEGYKRNLKLYSSERFSVGDTQNKFFIRLATCSSKNVEEFENG
                     LSILSNIIDECK"
     gene            1760..3160
                     /locus_tag="KPH93_002359"
     CDS             1760..3160
                     /locus_tag="KPH93_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG1689169.1"
                     /translation="MKNKKYISPILENIRNTDFTNDLTIAHNYAVEYINTVDNMRVYP
                     DKKALNNLSRFDENLCKTPTSNAEILHQLGKYGSKATVAQSGGRYFGFVCGGILPSSL
                     CSKWITDVWDQNPALYVLSPISSKLEDICEKWIVKLLGLPENTSVGFVSGSSTAIICG
                     LLAARNYLLNKLEYNVSKKGLFDSPKIKVVLSEGAHSTIFKSLSIIGLGQESIIKVPM
                     DEQGRILVAKIPPLDNSTIVILQAGNVNTGSFDNFEEVCKKAKDVGAWVHIDGAFGLW
                     AAANNTMSDLSKGIELADSWNMDAHKTLNSPYDNGIVLCRHRESLVNAMHMTDDYIVL
                     SKNRDSMMYTMEMSRRARAIDIWATLKGLGSDGVSELVEELHYKAKYFSDCLIKHNFE
                     ILNEVVFNQVLAYFKNDTATEELIRRIQESGVCWLGGSKWNGKSVMRISISSYKTTYE
                     DIDICIEEIVKLSKTL"
     gene            complement(3218..5992)
                     /locus_tag="KPH93_002360"
     CDS             complement(3218..5992)
                     /locus_tag="KPH93_002360"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG1689170.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDDEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEEQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKIEKSILDEIKKLQIKS
                     MRTLGFAYKKMSKSKTEVAITSEGELNLIGNQRGYMKEDNLVFSGFVGIVDPLREGVK
                     ESIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTINFNSKN"
     gene            complement(6164..7420)
                     /locus_tag="KPH93_002361"
     CDS             complement(6164..7420)
                     /locus_tag="KPH93_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG1689171.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLEILAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSTVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVFCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVVQIAGIMNKVAENIDNKE
                     ILDECKSEAQELISKFPLYPEGYFED"
     gene            complement(7492..8994)
                     /locus_tag="KPH93_002362"
     CDS             complement(7492..8994)
                     /locus_tag="KPH93_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG1689172.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(9085..10275)
                     /locus_tag="KPH93_002363"
     CDS             complement(9085..10275)
                     /locus_tag="KPH93_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1689173.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(10515..11603)
                     /locus_tag="KPH93_002364"
     CDS             complement(10515..11603)
                     /locus_tag="KPH93_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689174.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHLDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNIAPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCREKSTQSQK"
BASE COUNT         3794 a         2082 c         1482 g         4366 t
ORIGIN      
        1 ataatttata aactgcttta ctgcaagtga aatattcttt ttatatctta acacaatttc
       61 aatatttcga taaactgaaa cttccaactt ctttgctaca gtatgataag ggatatgttg
      121 taaaataagt cgtggtaatt tcaatatttc taactctaac atatagctat ttattaaact
      181 ctatattgat tatttcacga tgtacttgga ttatggatta attatcactt acattcatct
      241 ataatgttgc ttaaaataga taatccattt tcaaattctt ctacattctt agatgaacat
      301 gtcgctaatc taataaaaaa tttgttctgt gtatcaccta cagaaaaacg ttctgaggaa
      361 taaagtttga gatttctttt atacccttcc agttcaaata ttctccctgt aatacctttt
      421 ggcaaacaaa tccattggta ataagaattt tcgttatagc cttcatctaa atcaggaaaa
      481 tactttttaa atattttatt tctcctaatg gccattctac gcttatcaca aattatgtct
      541 tcatacatat cttttaaaat taattgcgtt gctatttcaa cattcaatga agaagttttt
      601 aaattacaac tatatactcc attttcaagt ttctttctaa atttatctgg aaaactcata
      661 tatgcaattc taagtcctgg acaaattgat ttagaaattc ctcctatata tactgattgc
      721 tctggaataa cagaatatat aggtgttaat ggattgtcta gaataaaagc ataaatatca
      781 tcttctatta aaattaaatc atgttttttt ataacatgtg caatttcttc tcttctattt
      841 tttcgcatag taatatttgt aggattacta caagaaggag ataaataaac tccatgtata
      901 tcccttattt tacataagtt atccaagctt tctggtaaca tacctttatc atcaccattt
      961 atggaaacta attcaatatg gagtaaattt gcaagcccta taaaatttgc atatgtataa
     1021 gtatctgtag caattttatc tccaggttta aaaagtgaaa ctaatgttag catcaaagca
     1081 ttttgtgttc catttgcaat aataatattt tcctcttttg cattaacatt gaatttatta
     1141 agatattttt ttgctgcttg tttttgtata caattaccta gaggatgtgt atattctaga
     1201 tatttatcag cattaggttg ttgcaaaatt tctattgcta aactttgaat aattttattg
     1261 tgattataaa atggaactaa catggccata tctatatttt tattaatttt ttcttctgag
     1321 agtattgttt tacttaaacc tgcattttga gatacaaaag ttccactccc tgctactcca
     1381 tacaataatc ctttttcttt acacaagtta taagcttttg taactgtatt aagacttata
     1441 tccaaaaaat ctgctagttc tctttgtggt ggaagtttta tcccctcttt caaaacgtaa
     1501 tcttcaatgt cctcttccat acattttgct aaagataggt ataatgggtt acttagtctg
     1561 tttttgttag gtttccatga catataataa ttatcaaaag aatttacagg catatttatt
     1621 cctcctaaat ttcttattat tttaattact aaaattgtac acagtacaat ttcttaattg
     1681 aatataatat aattcgaatg atattatcat attaacaata caatttagat gttacaattc
     1741 ttttttatag gaggaactta tgaaaaacaa aaaatatatt agtcctattt tagaaaatat
     1801 acgtaataca gattttacaa atgatttaac aatagctcat aattatgcag tagaatacat
     1861 taatactgta gataatatga gagtttatcc tgacaaaaaa gcgctcaata atttatcacg
     1921 atttgatgaa aacttatgta aaacaccaac atcaaatgcc gaaatacttc atcagctcgg
     1981 aaaatatggt tcaaaagcta cagttgctca aagtggagga cgttattttg gatttgtctg
     2041 tggtggcatt ttaccatctt ctttatgttc taaatggata actgatgttt gggatcaaaa
     2101 tcctgctttg tacgtattat ctcctatttc atcaaagcta gaagacattt gtgaaaaatg
     2161 gatagttaaa ttactagggc ttccagaaaa cacatctgta ggatttgtaa gtggcagctc
     2221 tactgctatt atttgtggtt tacttgctgc tagaaattat ttactaaata aattagaata
     2281 taatgtatca aaaaaagggt tatttgattc ccctaaaatt aaagttgttt taagcgaagg
     2341 agctcattct acaattttta aatctttatc gattataggg cttggtcaag aaagcataat
     2401 aaaagttcct atggatgaac aaggaagaat tttagttgct aaaattccac ctcttgataa
     2461 tagtaccatc gtaattttac aagcaggaaa tgttaataca ggttcttttg acaattttga
     2521 agaagtttgc aaaaaagcaa aagatgttgg tgcttgggtt catatagatg gtgcttttgg
     2581 gctttgggca gctgcaaata atacaatgtc agatttgtca aagggaattg aattggctga
     2641 ttcttggaat atggatgctc acaaaacatt aaattctcct tatgacaatg gaattgtact
     2701 atgtcgccat agagaatcat tagtaaatgc aatgcacatg acagatgact atatagttct
     2761 tagtaaaaat agagattcta tgatgtatac tatggaaatg tctcgtagag ctagagccat
     2821 tgatatttgg gctactctaa aaggactagg tagtgatggt gttagtgaat tagttgaaga
     2881 acttcattat aaagcaaagt atttttctga ttgtctaatt aagcacaatt ttgaaatttt
     2941 aaatgaagta gtattcaatc aagtattagc ctattttaaa aatgacactg ctacagaaga
     3001 gttgatacga agaatccagg agtctggtgt ttgttggtta ggtggttcta agtggaatgg
     3061 aaaatctgtt atgagaataa gtatctcttc ctataaaacc acttatgaag atatagatat
     3121 ttgtattgaa gagatagtta aattatctaa gaccctataa tataatttaa agactgcaaa
     3181 atcgtaatat gcagatgatt ttgcagtctt ataagaatta atttttacta ttaaagttta
     3241 tagttctact atttttcatt gttctaagta ttagctttac aaattcattt gacaatagga
     3301 tagtggatgc aaatagtatt atttttatcc acatatcaaa gtccaaagaa acagaattaa
     3361 agaaactctg gaacacttgt gtaaatatta tttgaattat ccctgtaact actattattt
     3421 gcaatgccag tttattttta aagaaatttg gtattgtact atttaaacca aactctctac
     3481 aatttaatgc attaaaaagg gcactgaacg caaataaaga aaatataaca gtaccttgtt
     3541 cttctgatgt agctcccaat atattaaaag ctgattgagt aaatacaatt gtaattataa
     3601 gaatagcatt tataataata tttacaaaca tagacctagc tattattcca gaatgtctat
     3661 ttattggttt tcttttcaat acataatctc taacaggttc taatcctaat gccagggctg
     3721 gaggaccatc cattattata tttacccata ataattgtat tgtagtaaat ggcatatcct
     3781 tgccagtaag ttgagatatt atagcaatta taaatgctac tatatttaca gtaagctgaa
     3841 actgtataaa tctttgaaaa ttctcatata tacctcttcc ccattttatt ccctctacta
     3901 tagtactaaa gctatcatca gtcaatatta tatctgctgc atttttagat acttctgttc
     3961 ctgaaatacc cattgcaatt ccaacatctg cttgactaag agctggggca tcatttatac
     4021 catcacctgt tacagctaca acttcaccac ttttttgaag tgctgaaact attctcattt
     4081 ttgtatctgg tttacttctt gcaacaatag atattccttt gatttcttct ctcaattctt
     4141 tatctgtcaa tacatctata tatgttgctt caactgcctt tttcccatca tttaataatc
     4201 ctaattcatt tccaatagcc gttgctgtat tgatattatc cccagtaagc atttttacat
     4261 ctactcctgc attaaatgct ttatcaatgg actctttgac cccttctctc aatgggtcta
     4321 ctattcccac aaatccacta aaaactaaat tatcttcttt catataacct ctttgatttc
     4381 ctattaaatt caattcacct tctgaagtta ttgcaacttc tgttttagac ttgctcattt
     4441 ttttataagc aaatcctaat gttctcatag atttaatttg taatttctta atctcatcta
     4501 aaatactttt ttctattttt ggagttattg gaacaatatt ttttccttgt tgtacataag
     4561 aacatttttt taataatact tctggagcgc cttttgaaag taatatatct gaatcctctt
     4621 gacgaattaa tgttgacatt ttcttttctt ctgagctaaa tggtatttgt gatatcaaat
     4681 aagtttgttt tctcatttca ttatagttct tatcattgtg atataaaagt aatgcacact
     4741 ctgttgcgct tcctatatat ttaaatgaat tgtcttcttt ttctatatct gcagttgaat
     4801 ttactataca gttttgttca aaataactat tactttgata ctctcctcca cttatatatt
     4861 taccatcaac atatgctacc tctaccatca ttttgttttg tgttaaagta cctgttttgt
     4921 cagaacatat tacagaaaca gaacctatag tttcacatgc ttctttcttt gttactaatg
     4981 catttatctt tgccatcttt tgcattgtta tagctaaggt aatatttatc atagttggca
     5041 aaccttctgg aacagttgca actattaaag caatacaaac catatatgca tttttagcag
     5101 gttctaatga ttgtaagaaa gataatacac tgcttgtatc taaaattaac ataccttttc
     5161 tctgcatctg tataatcata aacatacaca acaaagtagc tattacacct gatattcctg
     5221 ctattttagc acccaacttc cctaatttaa tttgaagtgg agtttgctgc tcttcatttg
     5281 ataaattctg tgctatctta cccatctctg tcttatcacc agtatgagta acaaccatta
     5341 tacctctacc atatgctaca agagtccctc caaataccat attgacttgc tttgcaggta
     5401 ttgcatcctg ttcgattatt tctgttttag aataaactac ttccatatct aatacagcat
     5461 cagcattctt agctacatca tcagattctc ctgttagcat atcttctcta agtttcaaat
     5521 ttatagactg tataagtctt ccatctgctg gtatcatatc tccagtttct atatatacaa
     5581 tatctcctgg tactaaatca ttctttgata ttttaattat ctttccattt ctaagtactt
     5641 ttacttctat attttctgtt aattttgaca gggcatgagc tgctttttta gattttcctt
     5701 cagttattat acctatagaa atcccaatga aaatagcccc taaaatacct aatgcatcat
     5761 gtaattctcc tacaaaagaa cttattactg ctgctcctat gagaattaat atcataggct
     5821 ctgtcaaact ttctcccaac tcgtcccaaa atgttcgacc ttcttttata gtaaattcat
     5881 ttaatccata tctaagcttc ctttcttcta cctcatcatc atcaagtcca atttctggat
     5941 ttgtttttaa gtattttaat acttctttgg taggtttatt ataatacctc ataactttat
     6001 cccccttgtt aattatctat aaggaattat atgcttatga tagcttgtat attacttttc
     6061 aaaaaaaata aggtgttgat taatcaacac cttatagtag ttatatatta ttaggtttta
     6121 cgttataggc ttcataagtt gcgggctata taaatcaata cacttaatct tcaaaatatc
     6181 cttctggata taatggaaac ttagatataa gttcttgtgc ttctgatttg cattcatcta
     6241 atatttcttt attatcaata ttttctgcta ccttattcat tatccctgct atctgtacaa
     6301 cttctttttc tttcagacct ctttgagata ttgatgtaag acctatacga acaccactag
     6361 ttacaaatgg tgattgtggg tcatcaggta taacattttt attaactgtt attcctacat
     6421 attcaagggc tttttcaaat tgttttcctg taagattctt acttcttaag tcaaccatta
     6481 ctatatgatt atcagtacct ccacttacaa tactaaaacc atgtgaaatc agttcagaag
     6541 ctagagtctg agcatttact acaacttgct ccattatctt tttaaattta tctgtactta
     6601 aatataatag tgaaaaggct tttgctgcaa ctgtattaag atgtatagaa cctaatgttc
     6661 ctggaaaaac acctttatcg agtttctttg catgttctgc tttacaaaat acaataccac
     6721 ttcttggtcc acagaaagtc tttgttgtag atgaagaaac aaaatctgca taaggtacag
     6781 gactaggtat tactttcgct gcaactaatc ctgcaacatg tgccatatct accataaagt
     6841 aagctcctac ttcttttgcg acctttgata ttctttcata atctattaat cttggatatg
     6901 aacttgcccc tgcaattata agttttggtc tacattcttt tgcctttgct tcaagagcat
     6961 cataatctat acgtccagta tttgggtcta ccccataaaa ttcatattta taaatatcat
     7021 gtaaaaaatt aactgtgcta ccatgtgtca agtgtccacc ttggtcaaga cgcatactta
     7081 aaacagtgtc atttggtttt aatattgatg aatatacaca atagttagct gtagaacctg
     7141 aatacggttg tacgtttaca tgttctgctc caaatacttc ttttgcacgt tttattgcta
     7201 ggatttcaag tttatctgct tcttcagaac ctgcttgaaa acgagcccct ggatagcctt
     7261 cttctgtttt atttgtaaat acacatccac ttaattcaag tacttctgtt ggtgctgtac
     7321 tttcagaagc tatcatctca atattgtgtt cttgtctaac caactcatca gctacaatct
     7381 tgtataactc tgggtctgaa atttttgttg tttttaacat aataatcctc ctcttaatta
     7441 aagaatatat tttattagtt taatcttcat tgataaattt attataacgg tctataaaac
     7501 gtaagatatt cctccaccag gtcctaatgg tatgccaata cccatgaata gtaacaatat
     7561 tattatttct attaatgtaa ataccactga gtatggaatc atattcgata tgacagtacc
     7621 tatacctata tctttatcat atcttctggc aaaggccagt ataactggaa aatacggaaa
     7681 aagtggactt attggatttg ttgaagcatc tccaatacga taagctattt gagttaatgc
     7741 tggattaaag tctagtagca taaacatggg aacaaatata ggtgctaaaa tcgcccattt
     7801 tgcagaagca cttcctacaa ataaattgat aaatccagat agcaatataa atcctattat
     7861 caatccatag ctaggtattc ccgactctgc tagtaatttt gctcctgtta ttgataaaat
     7921 tgttcctaag ttactaagat taaataaatt aataaactgg gatgctataa atgctaggac
     7981 tatatatcca cccatatcac tcatactact acccatcata gatactaaat ctttgtcact
     8041 ttttatcttc ttacttactt ttccatacac taatcctgga gttaaaaata taatagtgat
     8101 tataggaact atacctttca ttaaaggtgc attactagat agtatagaac cagtctctgg
     8161 atctttcata aatgcattgt cacctataca taaggcaact atcactacta ctacaactaa
     8221 caatgataat aaagcgtatt ttataccctt attttcaagt tcactcaatt cctgtatatc
     8281 cttatcattt gttgaatcat tatattttgt aaaacgagga gctataaatt tttcagttac
     8341 aaatgctcct gcaaaagtta aaactatcgc tgatataaaa gcaaaataaa gattcattgc
     8401 tggattgcct tggtaagatg ggtccatcat ttgagctgct ggtattgtaa atccagctaa
     8461 caatacatct gtagtactaa gcataacatt tgctgcaaat ccccctgcaa cacctgcaaa
     8521 agctgcaaac atacctacaa gaggatgtct tccaattcct aaaaacacaa gtgctgcaag
     8581 tggtggtaat attataaatc ctacatctgc tgctgtatgt gacaacatac ctgcaaatac
     8641 gattgctaca gttacaagtt tttgtggaac tttacttata ccctttttca ttaacacttc
     8701 cataaatcca gatttctcag caacacctgc tccaagcatt gtaaccagta ctaatcctaa
     8761 aggagcaaat gactgaaagt ttgtaactat gctacctaaa tatgattgaa tctgctcttt
     8821 ctctagtaga tttactacct ttacaacttc ttttgtagaa ggatgtattt cttctactcc
     8881 taaattagat ataactgctg acaatattac aacaattata catagtaata aaaatatggt
     8941 tactgggtct ggaagtttat ttcctgctct ctctacaaca tttagtgccc tcatcaataa
     9001 tccactatct ttgttttttt cctgtgaatt aattttcctc aaactaatac cccctcataa
     9061 actttatctc cagaatgctc tatcttaaat tttatttttt aatttatcca tataatttat
     9121 aacagactga catagtacat ttgtacctac taatatagaa tttttatcag ctttaaattt
     9181 aggattatgc cactgatata tacaatcttg ttctttgttg cctaccccta accagtaaaa
     9241 aaatcctggg gctttttcca tgaatataga aaaatcttct cctcctgtag aaggtattgg
     9301 atcaactata gcatcttctg aatacaaatc acacacagac ttacaagcta ttgcgtacat
     9361 ctctttacta tttataacag ctggtaaatc aaataaataa tctagctttg cagttgcttg
     9421 atatccacga gctatctcac aaactatatt ttctaaattt tctgatattt tctttcgtac
     9481 ttcattacta aagcttctac atgtgcctgt catatctacc ttttctgaaa ttacattatt
     9541 tgcagtccct ccatttatag tacctattga tataacagct gattctaatg gacttatgtt
     9601 tctgcttact atggtttgaa tacccattat aattgcactt gatgctacta tagggtctat
     9661 tgttctgttt ggtatacctc cgtgacctcc aaatccattt acttctatct taatagtgtc
     9721 tactgctgcc attaacccac ctaacttaac acctattttt ccacaaggta tatctggatg
     9781 attatgaagt ccaaatatag catcaacttt tggattttcc attactcctc gttccactag
     9841 catcttcgca cctttattga cttcttcagc tggctgaaat ataaacttta catttccatg
     9901 aagactatct ctaatatgag acaatacata tgcacatcct acaaggcaag atgtgtgtat
     9961 atcatgccca catgcatgca ttataccatc atatctggat ttatactcaa tatcaacctc
    10021 ttcatatatt ggcaatgcat caatatctcc tcttagcgct actgtgggac catcatactt
    10081 cccttttaat aatccaacaa ctcctgtttc cattcctaag tctaatattt ctatgtctaa
    10141 acttactaat aaatctttta tagcttttgt agtcctaaat tctttaaaac ttaattcggg
    10201 gtgcatatga atatctgtaa aaaaatctaa aatatcttct ttatactttt ctgagattaa
    10261 aataagttca tgcatactaa cacctctcca aaattttatt tttttatttt tttataattt
    10321 aactacaaat tataaaagtt aataatcttt ataaagatat tttttataaa tttaatttga
    10381 gtttatattt atattttact atattatttt aattaaatat attatcatgt tttgcaattt
    10441 atttaactat tttgactttt taaattcgga ttatataaaa aatttcttta aataaaaact
    10501 attaataagt ggctctattt ctgactttgt gttgattttt ctctacatgt aattgcttta
    10561 tataccatac agactaaaat taaaagcccc atataacctg tatatggata taaaatgttt
    10621 actagtttac caaatggtaa aaaaccacct atacaagcca aaatagctat tactgttgtt
    10681 accaatttaa atttaggatg ttcatcttca acaattctat tggttacaga ccacaaaagt
    10741 ggaacagctg ttgtatagat acctaatatt aatgcaactg aaaaacatac acctacaata
    10801 ggagctatat tatctgctaa ataaagtgat ggtatttctt tcatgtacaa atttcctata
    10861 tctgaaatca tagccaaata cataactata ccagcagtca taaacactat tccacctaat
    10921 aaacctcccc aaaaagcatc ctttttactt tcaatagaag ctcccattcc tgttaaaaaa
    10981 acaatcatta caataatatt taatgtatta tatattacac cagaaacgat tggactttct
    11041 gatgcttttg taacttcaat atttttcata gcttctccaa ccatagataa accatcaaga
    11101 tgcttaaaca agcttattaa tccaacagca attgtaaata ctgctatcac tggtccaata
    11161 cgtccaagta tgcttactaa tttttctaaa cccatagaca ctgtaattaa agtagcaaca
    11221 gccatcaaca ctcttcctaa gtaaggattt ataccatagt attcagttaa ggttgagcct
    11281 gcacctgaaa tcattactat aagaatccca aataaaaata ttggactaaa ccattcgtaa
    11341 aactttccta aaatctttcc acaataaaaa gtgtaaattc ttgttggttc tttaagtttt
    11401 aattctctac ctctagttat aacttcagca ccaagaaatg aaaacaatac cattgatatt
    11461 agtgctccaa ttaaacccat atatccttgt gaggcaaaaa attgtagaat ttcttgacca
    11521 gtagcaaatc cagaaccaat tgctgtagct aaataagcac ctgcaataca gataatattt
    11581 ttcatattca ctttgtctga catattaaat tacctccatt ataaagttac cttcctctaa
    11641 gaaatataaa ctctcggtaa attaaatgaa aattctgtct ttttagaata gtatttaatt
    11701 cacttttttt attttgtata tctt
//
LOCUS       DAEMAE010000115        11695 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEMAE010000115 DAEMAE010000000
VERSION     DAEMAE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.117"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            101..1033
                     /locus_tag="KPH93_002365"
     CDS             101..1033
                     /locus_tag="KPH93_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG1689175.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYVKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVVLKRVWIIKEMPEPSLPSIAILAAPGSLLLAGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            1138..1605
                     /locus_tag="KPH93_002366"
     CDS             1138..1605
                     /locus_tag="KPH93_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG1689176.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEYAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(1849..2703)
                     /locus_tag="KPH93_002367"
     CDS             complement(1849..2703)
                     /locus_tag="KPH93_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBG1689177.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMVSGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSFKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTFGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAILIGIILIMSG
                     VFMLSIIK"
     gene            2869..4833
                     /locus_tag="KPH93_002368"
     CDS             2869..4833
                     /locus_tag="KPH93_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1689178.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     RNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEEISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETNTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCFTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            4833..5294
                     /locus_tag="KPH93_002369"
     CDS             4833..5294
                     /locus_tag="KPH93_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1689179.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALNLANSEQEIINIFSEEGEM"
     gene            5297..5617
                     /locus_tag="KPH93_002370"
     CDS             5297..5617
                     /locus_tag="KPH93_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG1689180.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            5633..6733
                     /locus_tag="KPH93_002371"
     CDS             5633..6733
                     /locus_tag="KPH93_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG1689181.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            6853..9453
                     /locus_tag="KPH93_002372"
     CDS             6853..9453
                     /locus_tag="KPH93_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG1689182.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESHIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNNILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRKNVLEETPAGNVFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGRLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKALGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNIKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     VLTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQILKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEVISLPENY
                     LISACYPSYYSKDKFIIRLENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            9785..10978
                     /locus_tag="KPH93_002373"
                     /pseudo
     CDS             9785..10978
                     /locus_tag="KPH93_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032602.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(11180..11623)
                     /locus_tag="KPH93_002374"
     CDS             complement(11180..11623)
                     /locus_tag="KPH93_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1689183.1"
                     /translation="MFRYVHTNIIAKDHQKLIDFYKDVFHCKSIGETRDLRGEWLDNL
                     TGIPNAHILGEHLCLPGYEKDHPTLEIFSYDTMENSCNAINKCGIAHLAFEVDDVEET
                     LQLLLQKGGSQVGELVKTKYEDGKKAVFVYATDCEGNIIELQSWS"
BASE COUNT         4570 a         1379 c         1940 g         3806 t
ORIGIN      
        1 agatattttc tatataagaa agatggtcat aatatgtcaa tttgaccata tttttgatat
       61 aattaaaaca caaataattt agtaacaaag gaggcagaac ttgaatttat taaaaaaata
      121 tcctatacct attgcaagtt taattttatc aatctttact ttaggtaact tattacaatc
      181 ttatagtgaa tcattacgta atgtaattgg atttatagga tttattttgt atgcaattta
      241 tatagtcaaa ataatattac ttagaaaggg tgttaaagag caattagaaa atcctttaat
      301 atcaagtaca tttccaacta taacaatggc aacaatgata tttgctacat atgtaaaacc
      361 attatcttta gaattaagta ttatgatttg gagcatagga attgtgggtc atcttgtgtt
      421 aatagtctta tttagtaaga agttcttagt taaattctca ataaaaacgg tatttccatc
      481 ttggtatata gtatatgttg ggatagttgc agctagtgtt acagccccag catttaatca
      541 acttttgatt ggcaaaattg ctttttggat aggatttgta tcttatatag ttcttatacc
      601 agtagtttta aagagagtat ggataatcaa ggaaatgcct gaaccatctt taccgagtat
      661 agctatatta gctgcaccag gttcactatt attggctgga tatattaatt ctttttcaga
      721 taaaaataat gtagtgttat acttgttatt catattatca attgtatttt atattatagt
      781 tttgggatac ttacccaaat tattaagatt gcctttttat ccgagttttg ctgcatttac
      841 attccctatg gcaataagtg caatgggtat gaaactaatg aatggttatt taactaaatc
      901 aggtcatttt ataagttgga tttcatatgc ttctaagttg gaagagataa ttgctacagt
      961 gattatttta tatgttttag ttttatttat tatatatctt agtaaatctg aaactacatc
     1021 taaagctaaa taaaaattat taaatatgtg tatttaatag gaatattgat ataaatgtat
     1081 gaagataaga taaaccatag aaacttaatt tagcttaatt ttattggagg aaaacaaatg
     1141 aaaacaaaag aagaatattt aaaagaagtt agaaacatat cagaagaata ctttagaaaa
     1201 ggagagtttt tttgctctga agcagttctt cagacaataa atgatgcttt aggtcaacct
     1261 ttaagtccag agataactaa attagcatca ggatttccga taggacttgg aaaagctcaa
     1321 tgcctttgtg gagcgatatc tggaggagag atggctttag gtatagtata tggaagagta
     1381 catggagaaa ctatgaatcc aaaaatgttt gaatatgcta aaggtttgca tgattatatt
     1441 aaaaaagagt atggtgcaac atgttgtaga gctattacaa agaagtggga tggtgataac
     1501 tttatgtctc ccgaaaggaa agagcactgt attaaaataa caggacaagt tacagagtgg
     1561 gttgcagata agcttataga agatgaaaaa cttaatatca aatagtgcaa tattggttgg
     1621 tgaatttata aagttaaatt tacttagagt ataagaggct tggtattctc gtattgatag
     1681 aaaaatttat agcatatgta tttaatgaaa tatactttca tttagagtgt ggtttaattt
     1741 cataaataca tatgctatta ttttaagtat ataagcaaat ttatttaaat aaatagaaat
     1801 agttaattta tatgtacaaa aatatatatg attagtgtaa ataaatacct attttataat
     1861 actcaacata aagacaccag acatgattag tattatacca ataagaatag caagccattc
     1921 ttttttgttc ttcttttcct taaaaaatat aattcctccc aaagtggcta ctatcacaca
     1981 actctgagag atagaaaaac ttattgtaac tccaaatgta ttagttgtta tgaacatacc
     2041 taaatttgct attgaccaca atattccagt aattaaattg aataaaacat tttccctata
     2101 tacaatctct ttagaaaaat acttgacaat aatagatgaa gttatcaaca taccaacaga
     2161 ttgaggtaat ataattctat atccttcaat taaaaatatt tgattagtta caacatataa
     2221 tgataaaaat agagatgata ataatacaac tccaacacat tttaacatat ttttatctgt
     2281 agatttttta gtttgataat ttgtgagaag tataccaagt attatacata atattgataa
     2341 agaaccaatc agtaacattt taaaactggt ccattcatta aacaagatta cagctagtaa
     2401 tgtcgtaaaa gttaattgtg aaccatttga aatcggcatt actttagata cactagcaat
     2461 ttgaattgct ttaaactgca ttaattgtcc taaactccag aatatgccag aaaccatact
     2521 tatacaaaat gaaaaaaatg taatttcagg atgtataacc agagttatta tagtggaaaa
     2581 tataaacgca cttatggatg tacctaaaag ctgttcatga ggagaacttt tctttagatt
     2641 tgcaactatg ggcatcaatc cccatcccat tacaggaagt aaagcaataa ttattgttag
     2701 cattaaacta tttcctttca tattaaattt agacagcttt taatttatca tctaaaatta
     2761 aagtgttcaa tacataaaaa ttaccgtatt taatcaggaa atattagtag ttttttagaa
     2821 aaacattttc cattaaaata aagacaaagg gaaggagtaa aattgaaaat gttaactgat
     2881 atgattcaaa atcgtgaaat taaaataata aagtttttat taaaagaagg tcatacaact
     2941 gtaaaagcaa tagcagatga tttagatgtt tcagaaaaaa cagtttcaaa atctcttaaa
     3001 gaagtagaag cagtgcttaa aaagataggt ctttctttaa taagaaaacc taagataggt
     3061 gtttatattg atggagattt aaaaaaaata cttccttacc tagataataa aggaagtcaa
     3121 attcctacaa ccagagagga gagagttatc tatatatatt cagtactttt aaagcgtaat
     3181 gactatatta ctatacaaca actttctgac gaattatata taagtcgtgg aactattgaa
     3241 agggatttag cagaggttga aaaactgcta aaaaacgaag agatttccat atataagaaa
     3301 cctagtaagg gaataaagtt aaacataagt gaaagggata aaagacaact tactgctaag
     3361 tttatacata aattttggag tagaaactgg tatattaagc aagagcaaga aagcttttat
     3421 caagcatttg aaaagataca ggcagatgta aatggtattt tttcggaaga tgacttatca
     3481 ataattattg atattcttag gaatttcagc agagaaagaa aatttgagtt ctctgattat
     3541 gaatttcagt cattagttat tcatttggca atagctgtag agagaattag aaaaggcgaa
     3601 tatatagaag aaagtttgta tattggtaat atagaaaata atcaaatgtc aaataccaat
     3661 tatttagcaa aacaattaga agaaaaattt tcaatattat taccaaaatc tgaaattgga
     3721 tatataaatt tacatttagt agcggctgac caacatagag atactaatga actaaaaaat
     3781 acttcaacta atccttgtga aacaaataca aataatatat caaatcttcg ggaaattata
     3841 aaaaatacat tgtatgagac agcttatgac aaggatttaa ttgatggatt gacttcacat
     3901 gtacattcat caataaatag actgaaatat ggacttagta ttaaaaaccc atatgtaaat
     3961 acaatcaaac aaaacttttc tttatcattt gagcaagctt tagatttaaa gaaagtaatt
     4021 gaatttatat atgacattag gataaatgat gatgaatgtg cttatatagc attacatttt
     4081 gaagctttta gagaaagaat gaaagaactt ccacaaaata ttagagtaat tctagtatgt
     4141 agtacagggt taggttcatc taggttatta tctgcaagaa taaaaaaata ctttccaatg
     4201 ataaagattg aaaaagtact ttccgtacaa gaattagtat ccagtaagat tgatgcagat
     4261 ttagtaataa gtactattta tttagagtta gaaactattc caaccatagt agttagccct
     4321 attttaaatg aaagtgatat aagaaccttg gaaagaaata tacaaaatgt ttcatgtttt
     4381 acttctaaga agtataaatc tttcagaaac ttaatctttg aagagaatat attgttgaat
     4441 attgaagctt caaactatga agatgcaatt aagcatataa cagataacct aataaaaaaa
     4501 ggttttgcaa aagaaggtat agctaaaagt gcaattgaga gagaaaattt atcttacaca
     4561 tctttccaat caatagctac acctcatgct aatcctagtt atattaaaaa atctaatata
     4621 tctgttgcaa cgttaaaaaa tcctattaca tggggaaatg aaaaggtgga agttattttc
     4681 tttatttcat tagaaaatga caaaaaccta gagcttgatg aaatatatga agttttttat
     4741 gacttcattg acaataaaaa aaatataaat gacatattaa gtgcaacttc cacaaaggaa
     4801 gtgtacgatt tgctagtgga ggagagtata tagtgaaaat atcagatgta ttgaaaaaag
     4861 agcaaattgt ttttaatctt gatacaaata caaaggaaga aacaataaaa aaattaagta
     4921 gtatatttgt tgaaaatggg attgtggaca atgaagaaga atatgttaag tctgttttgg
     4981 aaagagaaga acattcaact acaggtattg gtaatggtat tgctattcct catggaaaaa
     5041 gtgatgctgt caaaaaatct tctataatat ttgcaaagac taagcataag attgagtggg
     5101 atgctttaga tgaaaaacca gtagatagta ttttcttatt ggctataagc aatattgata
     5161 aggatagcaa tcatcttgta ctattatcta aattagcaac taaacttatg gatgatgaca
     5221 atgtagatgc actaaattta gccaattcag aacaagaaat aatcaatata tttagtgaag
     5281 aaggagagat gtagagatga aaagaaaaat tatagcagtt acagcatgtg caacaggagt
     5341 tgcacataca tatatggcag ctcaagcatt aaagaaagct tcaaaaaaga tgggtcacct
     5401 tataaaagtt gaaacacaag gagctacagg aatcgataat gagttaacta caaaggatgc
     5461 acaaattgca gaagtagtaa tatttgcagt agatactaaa gtcagaaatg ctgaacgttt
     5521 tgaggggaaa gaaatactag aagtaccggt taatgcacct ataaaggatg cagagaaagt
     5581 aataaatgat gcactaaagt taattgacac taaataaatg gagggaaatg taatgttgaa
     5641 aaagatagtc tctgagttaa aaaagcatgt cttaactgga atctcatata tgattccatt
     5701 agtaattgca ggtgctatga taatggcaat atctcgtgta ggtggttctt tctataatat
     5761 accagatata tgggatgcta aatacgcaga aagtgctagt agtattgtta gattattaca
     5821 tgatttagat ggatttggag gtactgcatt aggattaatg tttcctgtaa ttgctgcatt
     5881 tataggattt tctatagctg ataaattagc tattgtacca ggtcttgttg gaggtatgat
     5941 tgctaaagat ataggtgcag gatttttagg agctttggca gttggtctta tagcaggata
     6001 cacatgttta tttattaaaa atcatgttaa attaccaaaa tcagcagctt caatagtacc
     6061 aatatttata gtaccagtat ttggaacatt aatcactgtt gtattaatca attatgtgat
     6121 aggtatacct tttgccacat taaatactgg attagaaaat tggttaaatg gattatctgg
     6181 tacaaatcag atattaatgg cagctattat aggggctatg atggcagttg acctaggtgg
     6241 accagttaat aaagcagctt tgacaacatc acttgcatta ttaacaagtg gaatttatgc
     6301 acctaatact gctgcaatgg taggaatagt gataccacca ctaggattgg gacttgctac
     6361 tatactagca aaacaaaaat acaataaaca gttacgtgaa gctgggaagt catctcttat
     6421 aatgggttta attggagtaa gtgaaggagc aattcctttt gcagtagaat ctccattaaa
     6481 agtaatacct tcatgtgtaa ttggaacagc aattgcttca gctatggcag ttggtttagg
     6541 ttcagtaaat gcaactccaa ttagtggatt ttatggatgg tttacagtag agaattggcc
     6601 aatgtatgtg ttatcaatag cagttggaac agtaattgta gctgggttag ttatagtatt
     6661 gcgtggaaat ccagtgattg aggatgatga atttgaagat gatgattttg atgaagcaga
     6721 gtgggaatct taaaatttaa taactattta agacgtgtta taaatcatat tcaatatgaa
     6781 tcttatacta aataaagtgt taaatattca gtttagactt aaatataaaa gataaagaac
     6841 agaggagaat aaatgataaa agcacatatt gttaatcata cacactggga tagagaatgg
     6901 tattttactt ctggagatgc attagtactt agtgaacaac tatttacaga tgtaattgat
     6961 gaattagaac gtaatccaga tgtaagtttt gtacttgatg gacaactttc tattttggat
     7021 gattatgtac aattacacaa agaaaaaata gaaatcatta aaaagttaat aaaagaagat
     7081 agactacata ttggaccatg gtttactcaa actgatgcat tctttgctag aggagagtct
     7141 attttaagaa acttaatgat tggaattttt gaaagtaaaa agtatggtaa gtatatgaaa
     7201 attggatatt tgccagatac atttggattt aatgcacaga tgcctatact attaaagcat
     7261 gtaggtcttg ataatatatt cttttggaga ggtatacatc ttggtaaaca ggttcaatca
     7321 ccatatttta agtggaaaag cttaaatgga gaaagtcata tatatgcagt aaatatgcca
     7381 catggatatg gaactggtat gcttttagag ccttcattaa agtacgttaa tggacgctta
     7441 gacccagcta ttgattttat taaatcatat actgatgtag atgaggtgtt aataccttct
     7501 gggaatgacc agttaaatat aataggtgat ttcaaaaata aaattaaagc gattaatgat
     7561 atcggaaagt attcatatat aacgagttca tatcaagagt ttttaaaata tataaaatcc
     7621 atagaatcgc tagaaagcta tagaggtgaa tttagagagc ctgtattagc aagaatacat
     7681 aaatcaattg gttctagtag aatggatata aaattagcat gtgataaact agaaaacaag
     7741 ctgattaaaa gaatagagcc attattagta attgccaaaa agagtaaaat tgaaataagc
     7801 aatcagttac ttataaatac atggaaaaaa cttttggaag gacaagcaca cgatagttta
     7861 gctggatgtg taactgatac tgtaacagac gatattcttc accgtataag agaagcaaat
     7921 gaaatatgtg acagtattga aaatactatt gtcaaaaaga tttcagaagg gcttaaatta
     7981 tctaaaaata atattttagt atttaataca gaagctaagc gttttaatgg atataaagag
     8041 atacaagttg taagtgatag taaaaatatt tattttaaag ataatttaga tgctactata
     8101 attgaagaaa cttatgtaaa accgagaaaa aatgtattag aagaaacacc tgctggcaac
     8161 gtatttattg aagaaccagg atattatata ttaaaagtaa gaataaatgt acagcttcct
     8221 gcacttggtt ataaagttgt tagttttgaa ttaagtgata aagaaatggt atcacttgat
     8281 aaatctaatg acacttttat atctaatgat aattataaga taatctatga agatggaaga
     8341 ttaaatttac aacttaaaga tggaacttac atatcaaact ttttaacatt aaaagatagt
     8401 ggtaatgcag gcgataccta tgacttttct ccattaaaaa atgacgagga tataaaactt
     8461 tcatttgata gagtgtatac agaaaaagct ttaggatatg aaaagttggt ggttaaaggt
     8521 actaccttac taccacttac attagaagat agaaaaaaca aatatcttaa tgggaaatta
     8581 aatataaaag taagtattgt cttatccaaa gaaaatccat tgatggatgt aaaactttgt
     8641 gttgataaca cgatatataa tcatagacta cgtgttcata taaaaactga tataaaagat
     8701 aataaaaaca tagcatcatt gccatttgga tatataacaa gagaaaatgg agttttagac
     8761 aactgggaag atatttatag tgaaatgcct atagatttag aacctttgga aagtaatatt
     8821 actctaacaa accaaaatag aagttgtact gtatttacta gaggaataaa agagtatcaa
     8881 catattgaag atgaaattgt attgacctta cttgctacta cagatgaact aggaaagcct
     8941 gatttattat atagaccagg acgtgcttct ggtgatacaa ctaaaaaagg tcatataaga
     9001 attaaaacag ataaagctca aatacttaag aaattagaat tttcatttgc aatatatatg
     9061 gattctaaat catttgatga attggaaatt gcgaatttaa cccacaatta tctaagggaa
     9121 tgtgtaaatt atcaattaca ggattacaat ttctttttat atcgtataga caataagatt
     9181 caaaaatcaa ttgtcaataa aactgtaagt agagaattag aagtaatatc tttaccagaa
     9241 aactatctta tatcagcatg ttatccttct tattatagta aagataaatt tataataaga
     9301 ttggaaaatc caagtagtaa tagaatgata ttggatgagc aaatatttaa aaatagaaat
     9361 gggaaaattg taaatgcaat agaagatgta gaagaagaac aggtatatga aatagcacct
     9421 tttgacgtta tttcaatatt attggattta taagtgtaaa agtaagttta ttgtttaaag
     9481 gggctgtcgc attagcaaat taaatttgtt ctgtgacagc tttttttact tatcttaatg
     9541 gggacatttt taattgtttt atatagagtt tatgttctaa ataaaattat gcataacaaa
     9601 taaaggcata aaaatttttt tcagataata tttagtaaat ttagaatact tttttttaat
     9661 gatgaagata ttccgggtct ttaaaataaa gtgtgtattc ttctaaaaaa tggtgataat
     9721 taaaatatta aatatcacta tataaaggaa ggtgcatact ttttatgttt aacaaaaacg
     9781 aaatattaaa aaacttattg aagaatacga tgttaaaaca actacagaca ttcaaaatat
     9841 gttaaaagat ttatttgcaa gtacaattca tcaaatgtta gaagcggagc ttgatgacca
     9901 tttaggttat gatagatatg ataataagaa taaaaatact aaaaattcta gaaatggata
     9961 tagaaataaa aatgttaaat cagattttgg agaaataact ttaaatattc caagagaccg
    10021 aaatggagat tttgaaccaa aggtaattag aaattatgaa aatgatatat cgggagtaga
    10081 gaaccaagtg attggaatgt actcaaaagg catgtctact cgagatatat caagccacct
    10141 taagagtatt tatagaattg atatatctca ttctctaata gctaaaatta ctgatagagt
    10201 tctaccttta gctcatgaat ggcaaaacag acctcttgat atggtttatc caattgtatt
    10261 tatggatgct atacattata aagttagaag tgaaggtaga ataataaaca aagctgcata
    10321 tatagccata ggtgttaact tagaaggtat aaaggaggtc ttaggaatat ggattggaga
    10381 aaatgaaagt tcaaagtatt ggctaaaggt acttacagat gtcaaaaaca gaggagttaa
    10441 ggatatatta atagcttctg tagatggatt agttggattt tctgatgcaa ttaaagcagt
    10501 atttcctgat actgaaatac aaatatgtgt tgtacatcaa attagaaata ctttaaatta
    10561 tgtatcatat aaaaatagaa aagaatttgc aaaagattta aagaaagtat atgctgcaat
    10621 tacagaggaa gtagcattaa gtgagctatc gctcttagag gaaaaatggg gtaatcaata
    10681 tgcgatagca ttaaaaaatt ggcgaagtaa ttaaaatgag ctagcaacat accttaaata
    10741 tcctcaagag ataagaacat tgatatatac tacaaattca atggaaagtt acaatatctt
    10801 attaagaaaa gttactaaat cgaagtctat ttttccttca gatgattctt tgcataaatc
    10861 attatgctta gcaactatgg atatttctaa aagatggact caaaagatta gaaattggac
    10921 tcaaatttta gctcatttaa gtatttattt tgaagaaaga attttaactc taaattaata
    10981 aagaaatatt tcaaaaaaat taagaaattt tcttaaaggg gtaataattt ttgcaaaaat
    11041 ggaaaaaata tcaaaataag gctatgcttt ttaaagctat agccttacca ataattgttt
    11101 aattatcact taaaaaataa acttttattt aaggaatata cataaatatt tccagactca
    11161 atatgttcac actttatacc tatgaccagc tttgtaattc aataatattc ccctcacaat
    11221 ctgttgcata gacaaaaacg gctttctttc catcttcata ttttgtcttt accagttctc
    11281 caacttgact gccgcctttt tgaagtaaga gttgtaaagt ctcttctaca tcatccactt
    11341 caaatgcaag atgtgctatt ccacatttat ttatggcatt acaagagttt tccattgtat
    11401 cataagaaaa aatttcaagc gtaggatggt ctttctcata tcctggtaaa caaagatgtt
    11461 ctcccaatat gtgtgcatta ggaattccag ttagattatc aagccattct ccacgcaagt
    11521 cccttgtttc tcctatgctt ttacaatgaa atacatcttt ataaaaatct atcagttttt
    11581 gatggtcttt agcaataata tttgtatgta catatctaaa catatatttt cccctttcaa
    11641 attctaattt gtaagaatat tatacaacta ttcaatacca ttagcaactc taaat
//
LOCUS       DAEMAE010000116        11659 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEMAE010000116 DAEMAE010000000
VERSION     DAEMAE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.118"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..353
                     /locus_tag="KPH93_002375"
     CDS             <1..353
                     /locus_tag="KPH93_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1689184.1"
                     /translation="LIAYFSIVYCVYKASNLRGVSYVHIMTLQVFLYMAISPIPTPGN
                     VGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGIFTFGTHYTLNSWKKEN
                     KVNSNLDKVIEKLN"
     gene            502..663
                     /locus_tag="KPH93_002376"
     CDS             502..663
                     /locus_tag="KPH93_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG1689185.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1004..2272
                     /locus_tag="KPH93_002377"
     CDS             1004..2272
                     /locus_tag="KPH93_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG1689186.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            2467..3255
                     /locus_tag="KPH93_002378"
     CDS             2467..3255
                     /locus_tag="KPH93_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG1689187.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGCMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASRYYRKAMSKVLLKRAIMEVI"
     gene            3255..3704
                     /locus_tag="KPH93_002379"
     CDS             3255..3704
                     /locus_tag="KPH93_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG1689188.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDEEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            3708..5972
                     /locus_tag="KPH93_002380"
     CDS             3708..5972
                     /locus_tag="KPH93_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1689189.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMIDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVITPVLDFEEAIGNKVVVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHTGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKILNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLDVDKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6112..6423
                     /locus_tag="KPH93_002381"
     CDS             6112..6423
                     /locus_tag="KPH93_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689190.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            6709..7209
                     /locus_tag="KPH93_002382"
     CDS             6709..7209
                     /locus_tag="KPH93_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG1689191.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLI
                     IASLWPTIINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            7266..8252
                     /locus_tag="KPH93_002383"
     CDS             7266..8252
                     /locus_tag="KPH93_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG1689192.1"
                     /translation="MSKVKRLTDVWTYINDKQKFTVKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMTILFALKSLQIYNYKLIEIEINS
                     IMRKISYERKSSIKVGIENIKRYIGFVANDTNDGDFKSINVNEFFKASSESLILNIKY
                     KFGNQILEKNICPIGIYLNKGAWFSPAYDCKSDKITLVHIDNIIDIKRTGESKKIHIN
                     LEEWLYEVYNNLYGNSSTTKKIGETIYLNVLLTKEGVSKIKDSSWNLDMEINEDGSGI
                     ISTFIKVEDIDCYASILFLIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            8302..9024
                     /locus_tag="KPH93_002384"
     CDS             8302..9024
                     /locus_tag="KPH93_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG1689193.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEMADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLINNIQSKVEESYE
                     VIFSLYMKENLNGNIRVGLLYN"
     gene            9053..10987
                     /locus_tag="KPH93_002385"
     CDS             9053..10987
                     /locus_tag="KPH93_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1689194.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFNMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     NFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     SLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLENLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSTLEGGYFFPVRWIEYEKEHIDGNAWV
                     IFENNSIVEKYIKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALRFEKKM
                     SFELEKDSLTLETIKERKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDVRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            10980..>11659
                     /locus_tag="KPH93_002386"
     CDS             10980..>11659
                     /locus_tag="KPH93_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689195.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIVDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPII
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKRGIYFEYINKIENLNVIRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKK"
BASE COUNT         4683 a         1057 c         2093 g         3826 t
ORIGIN      
        1 aattaatcgc atatttcagt atagtttatt gtgtgtataa agcttcaaat ttaagaggag
       61 tttcatatgt acatataatg acattacagg tctttttata tatggctata tcaccaattc
      121 caacaccagg aaatgttgga gctaatgagg ttacattttt tactatgttc tccaatataa
      181 ttccaaaaga attattgggg tactctgtac tcttatatag tgggtttgta tactatttta
      241 tattaatagt ttcaggtata tttacatttg gaacgcatta tacattaaat tcttggaaaa
      301 aggaaaataa agtaaattct aatttagata aggttattga aaaattaaat taataaaata
      361 attagggaaa ttctatatat aaatatggcg ttgaataagc tatattgcat tgagatattt
      421 cccttttttg attaaaatta tagaaatttg aaataataag taataaacag aaagattgat
      481 attgttttga aaggagaaac tatgaaacaa gataaaaaaa atgaaaaaca aagtgagatt
      541 tctaagataa aacaaagtaa tgataataaa cttgaaatag ctcaagaatt acatgtaagt
      601 aaaaatatag ggaatatgct tggatataga atagataaga aaaaaatgaa tagaccaagt
      661 taatgaaaat gttgaaaatg gtacatttta aaaaatatat ataatgaaat gttttattgt
      721 taaatttaaa cttattttaa tgaggaaaaa agtcgaaaaa tatctaaaaa gatatggata
      781 taaaaaatat taatatatat atactataaa cttgacaaaa tcaacctttt aataactagc
      841 aaaaaatagt aaaattatat aagcgttatt taattgtcaa aaatgaaagc taaattgcat
      901 gtcctaaaaa aaatgattga taacatttat ttgtgtgcta taattatata gtaaagcaat
      961 aagtatattg cataatgtat gcaaattggg aggagagacg gacatggaag catctataca
     1021 cacaattgcc agcaataatt tattgataat ttttgctatt gtaagtataa ctggaataat
     1081 ttgtagtaag cttagtgaaa taataaaggt tccagatgta gtgttatatt tacttgtagg
     1141 tattttaata ggaccatcat ttttaaaatt tatagatatt cgtggatttc aaatagaaaa
     1201 tcaactaata cttacatttg gttctgcatt tattttatat ttagggggaa aagaaataag
     1261 tttaaaagta ttgagaaatg tgaagatatc tgtattctta ttagctactt taggagttgt
     1321 gatatctgct tttataatgc aacaagtaat aggatttact tttgggataa gtgcaatgac
     1381 tgctttacta gcaggaacta taatagcatc aacagaccca gctactcttg tacctatatt
     1441 taatcaggta aagataaagg atagggtaaa acaaactgta ataagtgaat cagcttttaa
     1501 tgatgcaaca ggagctatac taacatcagc agtgttggct gtaattttgt caaataaatt
     1561 ttcattaggt gaaaatattt atgaactggg gacaatggtt attgtcggag ttttagttgg
     1621 tttaattaca ggaatactgc ttttgaaatt agttaatgat aaaccttatg gaatatttaa
     1681 agattttgca ccaataatat ctgttatttc agttgtagtt gcatatgaat tatctacaaa
     1741 attaggtgga agtggatata tgtcttgctt tattgtaggt ataataactg gtaacaagaa
     1801 aaactttaaa atttggttaa gtcaaaaatc ttatgatgca gatttttatg ttgctgaaac
     1861 tttaggtact atttgtcgta tggcaatatt tataatattg ggtagccaag ttaatttagc
     1921 tcagttaagt aaatatttta tgccatctgt aattacagta ttagtattaa tgtttatagc
     1981 aagaccactt tgcgttttag tttgtacgct tgtagacaga gaagcaaaat ggagtaaaag
     2041 tgaaatatta tttatgatgt gggtaaggga aacaggagta ataccagcag cactttgtgg
     2101 tattatttca gcaatgaaag ttcctggata cgaagtaatt tcatctgtag tatttatgac
     2161 tatactaatt acattagtaa tacaaggaag cactacaaaa ttagtagcta aaaaacttgg
     2221 attattagaa gaagaagtga taaatataag tgaaaaagta tcaacttttt aaagttttgc
     2281 tttaaaatag tatctgctaa tggctatttt aatattgtct aatgatgatt attgaaatag
     2341 tcattttttt atgtataagt aatttacaga atttacatat tagacatatt tattatcaaa
     2401 atttgttgaa gctatgatat aattagttgt atttaaaata agaatagcaa ataagggagg
     2461 aaatttatgt ttacggttat ggatattgta cagccagata cagtagaaga agcgtactca
     2521 attttaaata aaagaaaaac taaccaagta attggtggaa gtgctttttt aagaatgggg
     2581 aaaaaaagaa taggaacagg aatagaatta tctaaattaa atttagatta tataaaagaa
     2641 tatgaagatt atgttgagat tggttgtatg actactttta gaacattaga aacaagttcc
     2701 ataataaaaa ataattttgg aagaataata gaagattcag ttaaaaatat aataggggtt
     2761 cagtttagaa atgtagtaac agttggagca acagtgtttt ctaaatatgg tttttctgac
     2821 ttgatagtag cattactttc attagatacg gaagtcgaat tgtataatat tgggagaatt
     2881 agtcttgaag agtttctaaa tagagactat gagaaagatt tacttattaa gatatacata
     2941 aagaagacaa ataagaatgc ttcttataaa tcacttagaa atgcaaagag tgattatcca
     3001 atcttaaatg tatctgtatc aaaacatatg gaacaattta agttatgtgt aggagcaaga
     3061 ccgcagaaag ctacgattgc aaaacaagct agtgaatttc tatctaacaa tgaaatcaat
     3121 gaaattaata tagataaagc agtagaaata gcatcagaag aactaacttt tggttctaac
     3181 atgagagcat ctaggtatta tagaaaagct atgagtaaag tacttttaaa aagagctata
     3241 atggaggtta tttaatgtta gtgaagatta atataaatgg gaagaaaaaa agtgttgata
     3301 tagaaccaga agaatatctg gttgatactt taagaaaagt tggtaattta agcgtgaaaa
     3361 gaggttgtga cacaggctgt tgtggtctat gtacagtttt aattgacaag aaacctacat
     3421 tatcttgtgc aacactgaca ttgagagctt tagataaaga aataactacc attgaaggtt
     3481 tagaaaatga agttaaagaa tttagtgaag ttttggtaaa agaaggagct gagcaatgtg
     3541 gtttctgttc tcctggcttt atacttactg tacttgcaat gaaggatgag cttgaaaatc
     3601 ctacagatga ggaaataaag cattacctta caggaaatct atgtagatgt acaggatata
     3661 tgggacagtt aagagctatc aaaacttatc tggaggtaaa atagaacatg aatatagttg
     3721 gaaaaggaat caagaaaata gatggaatga gtatcacaac aggaaaacct gtatatacag
     3781 atgatttatc agcaaaagat gctttggtgg taaagatact tagaagtcct cacgcctatg
     3841 caaaaataaa agatatagat acatcaaagg cagaaatgat tgatggtgtt gaatgtgttt
     3901 taacatataa agatgtacca aaatctagat ttacacttgc tggtcaaaca tatccagagc
     3961 catctccata tgatagatta attcttgaag atgttgtaag atatgtaggt gatgaagtag
     4021 ctatagtggc agctatagat gaaaaaactg caattaaagc aatgaatctt atcaaagtaa
     4081 agtatgaagt aattacacct gttttggatt ttgaagaggc aataggaaat aaagttgtgg
     4141 tacatgatga agatagacat tgtaattttg atataggtat gcaaagagaa aaaaatatag
     4201 tatctcatca tgattataca aaaggtgatg tagaaaaagt attaagtaat tgtgatgtag
     4261 tgattgaaga aacttattat actcaagctc aagcacatgc aatgatggaa acgtataggg
     4321 catacaatta tttagaccat acaggaagat tggttgtagt aagttcaact caaataccat
     4381 ttcatgtaaa aagacattta tctcgtgcac tagaaatacc agctagcaaa attagggtta
     4441 taaaacctag aattggtggc ggatttggtg gtaaacaaac tgcatgtgtt gagatttttt
     4501 ctgcaattgt tactttaaaa acagggaaac cagctaaaat aatttatgat agaaaagaaa
     4561 cttttaattg ttcaacaagt agacatgcaa tgagattaaa agttagatta ggtgctacaa
     4621 atgatggcat aataaaggca atagatataa aagcactttc tgatacagga gcatatggag
     4681 agcatgcttc tacaactatt ggtttggttg gagaaaaaac tttaccaatg tataataaag
     4741 ttgaagcaac aagatttatt gcagatgtgg tatatactaa taagatgcca gcaggagcac
     4801 ttagagggta tggagcatca caagggtgtt ttgctgttga atcaactata aatatgcttg
     4861 ctaataaatt aaaaatggac ccaacagatt taaggttaaa aaatgtggta acagaaggtg
     4921 aaactacatt tgcatatggt aaaattttaa atagtgtcga tttaataaaa tgtataaata
     4981 aaggcaaaga acttattgga tggaatgaaa aatatccttg taaacatctt gataatggaa
     5041 aaattagaag tgtgggtatg gcaattacta tgcaaggttc tggtatacaa ggtcttgata
     5101 agtcttcagc tgaaataaaa ttgaatgact gtggagatta tactcttatg ataggttcta
     5161 ctgatatggg tacaggaagt gatacaattt tagctcaaat ggctgctgag gttcttgata
     5221 caagcattga aaatataacg gttatatcag ctgatactga tttagttcca tatgacccag
     5281 gttcatatgc ttcaagtact acttatgtaa caggtatggc agtagttaag gcagctcaag
     5341 acttaagaga aaaaataatt tctgctggag caactaaact agatgttgat aaggatgaag
     5401 tagaatttga tggtataaaa ttaactagta aaaaaggtag cactttatta ttcgatatag
     5461 cagttgattc tacaattggt ccagatacta atcaattaat aggtcttgct tctcatggta
     5521 gcccagtatc tcctccacct tttatagcag gttttgcaga aactgaaatt gacacagaaa
     5581 ctgggaaagt tgatttagta aattatgtag ctgtagtaga ttgtggaaca gttataaata
     5641 aaaatctagc taagatacaa gttgaaggtg gaattgttca agggattgga atggcattat
     5701 ttgaagaagt tagatacaca gaaaaaggaa gcatggacac aaatacattt atgcaataca
     5761 agattccagc tagatgtgat attggaaatg ttaaagtaga ttttgttgaa acttatgaac
     5821 caactgggcc atttggtgct aaatctgttg gagaagttgt tataaataca cctttaccag
     5881 caatacaaga agctgtgttt aatggaattg gtgtaagaat aaatacactt cctattacac
     5941 ctgaaaaagt ttttatggaa atgaataaat gattatttat gaaaatttag actagtttta
     6001 catgttaaac aaccagttat gtcatacctg ttgaaagata tttaataagt agttattata
     6061 atattatgaa ttttacttat aaaatacaaa attaaataag gggatgatgt tttggctggt
     6121 aatctaaata atatgagagc agtaaataat tttagaggag ataagaacat tttagaatgt
     6181 ttagtcagct ttgagggtcg ttcaataagt cagagaaaag taagggtatt ttttaaagaa
     6241 aaacaaaatc aaatagaaat tgattttgca gaagaggaaa tttctaaatt ggttgaaaat
     6301 gttgttttaa atacatcata tcaagaaatg ttatatgatg aaatagagaa acaactggaa
     6361 attgattgta taggtacttg gatgatatta tctaaattaa aagatggtag tagagttcac
     6421 tgattttaaa ataaattatt agagcctaat tttaaggctc ttttttgctg tttttaatta
     6481 acaatagaaa atttatcttt aatttgtata taattttaat atcatatatt ctatatatat
     6541 aaaattgaat ttgtatgtat tatattgtat aatataaaat gaattaggaa aatagttcgt
     6601 taaaataatt tgtttttata aaattactgt ttaagtttat agattttgta taaaataaag
     6661 ttatgttatt aattttttta gaaataaaac ttttattagg agacagatat gagaaaaaat
     6721 agtattaata tagcactaac tgcagcaatt acagcaatct atgcagtttt aacattatca
     6781 ttgggattta taagttatgg accaatacaa tttagggtag cagagattat gatgcttttg
     6841 gcatttttag ataaaggata tatagtaggt cttacattag gttgtttttt agcaaatgta
     6901 ataggaccat atggtgtacc tgatataatt tttggaacat tagccacatt tataagtgca
     6961 agtatgattt atattactag aaagttaggt aaacaaaata aaagtacttt aattatagct
     7021 tctttatggc caactattat aaattcgatt gtggttggac taatgttaaa tgtattcttt
     7081 ggacttccat taattctatc catgatacaa gtaggttttg gagaatttgt agtagtaaca
     7141 attgtaggag taccattttt ttgcttttta atgaaaaaat ataaaaatgt cattaatatt
     7201 aaattttaaa tagtgtatag taatttaatt aattgagagt attattagtg aaaagaggtg
     7261 tttatttgtc aaaggtaaaa agattgactg atgtttggac atatattaat gataagcaaa
     7321 agtttacagt aaaagagttg tcagaggaat ttaatctttc agctaaaaca atacaacgat
     7381 atttaaaaga attaaataaa atgggacttc caattcaagc agaacaaggt agaaatggag
     7441 gttatagggt gctaaataat agctatatac ctcctgttat atttacagaa aaagaagtta
     7501 tgactatact atttgcactt aagtcacttc agatttataa ttataagtta atagaaatag
     7561 aaattaattc tataatgaga aaaataagtt atgagcgtaa gagcagcata aaagtaggta
     7621 tagaaaatat taaaagatac attggctttg tagctaatga tactaatgat ggagatttta
     7681 agagtatcaa tgtaaatgag ttttttaaag cttcttccga atctctaatt ttaaatatta
     7741 agtataagtt tggtaatcag attttggaaa aaaacatatg tcctataggc atatatttaa
     7801 ataaaggagc atggttttca ccagcctatg attgtaaaag tgataaaata acattagttc
     7861 atatagataa tataattgat ataaaaagaa caggagaaag taaaaaaata catataaact
     7921 tggaagaatg gttatatgaa gtatataaca atttatatgg aaatagttca actactaaaa
     7981 aaattggtga aactatttat ctaaatgtat tattaactaa agaaggcgtt tctaaaataa
     8041 aggacagttc ttggaattta gatatggaga taaatgagga tggtagtgga ataattagta
     8101 cattcataaa ggttgaagat atagattgtt atgcttctat tttattttta attggaaata
     8161 atgctaaggt tatagagcca aaatttctga ataaactact ttatgataag gccaatgagt
     8221 taagatattt ttatgaagaa aatatggtat aatatttaga aatgttgaaa ttaaataatt
     8281 attaaggaga tatacgttat aatggaaaat tttaataaga atgctaaaat aattggtatt
     8341 ggagctgaag gtattaatat aataaatgaa gtagaagaaa aaataaaagc aaatatggat
     8401 atagaaaaaa taaacataaa tcaagagatt gaaaaggaat atgttagaag tcttttagat
     8461 ggcgtagata tattattttt gatatattca tctgaagata aacatattag agatattata
     8521 aaagctgttt catatatgtc aaacgaaaga agagtcctat ctataggaat ggattgctca
     8581 gagaaagaaa ataaagaaga tttagagctt gggagagagt ttaaaatcaa taatgatagt
     8641 atatttaaat ttgttgattt aatgaatatc atggtagagt ctatttctga ttcttgcatg
     8701 ataaatatag atattactga tttaaaagaa gctattgtag gagataaagg aattaagtat
     8761 tcttttgaag agtttgaaga tacaaagtca tatagcgaga tggctgacat tttatttgac
     8821 aggatggaat atattggtga ggaatttata agtaagaaag gaattgtatt tgttgaaggg
     8881 agtccagaat tttctataat ggaattgaat gatttaataa ataatattca gagcaaggta
     8941 gaagaaagtt atgaagtaat attttcttta tatatgaagg aaaatttaaa tggaaatatt
     9001 agagtaggtt tattgtataa ttagtatatt ttagatatta aggagagtgt atatgtctga
     9061 aaatataaga ggtacagagt attggctaat ggaagaattt ttttatcagg aaacagatac
     9121 accacagatt tttagagaag aaagtataat tgcacttgga tttgatataa aaataaattt
     9181 aaaaaaagta ttcaatatgg gaaaaaaatc agaaaagtat attaatgaga attgtaatga
     9241 taaagatttt ataaaaaagt ttatgtcgat taaaaaagga gattattttt tatataaagc
     9301 atatcagaat atagaagaaa aagttaaatg taatgtgtct attggcatag ttgaagaaaa
     9361 ttttgaaaat ggatatgaat taagtcatat tttagggcat acactaccag taaaatggat
     9421 gcataatttt gatacagttt caaattgtaa agtatataca agtgaatttt ataaattaaa
     9481 taataatgat attagctcat atttagaaat tattaagtca caagataaga aatcattgag
     9541 aaaaagaata gaagaaagtg aaaataataa atggtattta aaaaactatt attataaaaa
     9601 tgtagataag tgtgtagatg atgaagaatt atttattcag cccattaatc tagaacaatt
     9661 agaaaaccta aaaaatataa agacagatga ttatatttta ttgtataata gcgagaaagt
     9721 aatttgtgaa aatgaccata caatgaagag tctatttggc gtatgtcaag ttatggaagg
     9781 gttcgataaa gagaaagtaa gtactttgga gggtggatat ttttttcctg ttagatggat
     9841 agagtatgaa aaagagcata tagacggaaa tgcatgggtt atatttgaaa ataatagtat
     9901 agttgaaaag tatataaaaa gatttactaa taaaatgcca ttaaatacta tactttatgg
     9961 accaccagga actggtaaga catacaatac aaaacagatg gtttacaatc tagtaagtga
    10021 aactagtcta agaaataggt actatacagg ttacttagat tataaaaaca atgtagaatt
    10081 ttgtacattt catcaatcat atggatatga ggaatttata gagggcctta agtctgatgg
    10141 tgaaggcaat tttaaaccag aagatgggat attaaaggat atatctatag aagcatgtta
    10201 taatgcattg agatttgaaa aaaagatgag ttttgagttg gaaaaagata gcttgacttt
    10261 agaaactata aaagagagga aaaagaaatt agtattagaa aatatagaat atagtgagag
    10321 ttttaatttt gaagaatcag aaaattatgt tttaataata gatgaaatta atagaggaaa
    10381 tgtatctaaa atatttggtg agctaataac attattggaa gatgataaaa gacttactaa
    10441 atcaaatcaa actattgtca aactaccata ttcaaaagaa aaattttctc tacctccaaa
    10501 tttgtatata gttggaacta tgaacacatc agataaatca atagcacttt tagatatagc
    10561 tttaagaaga aggtttaatt ttgaagaaat tatgccagat tatgatttat tgtctattgt
    10621 agatggtata gatgttagaa aaatgctata tacaatcaat aaaaggatag agtttttata
    10681 tgatagagac catgttattg gacaagctta tctacttgct gtagaaaata ttaatgatat
    10741 agtagtagta tttagaagta aaataatacc acttcttcaa gagtattttt attatgattc
    10801 agaaaaagta ggtttgattt taggaggaat tggaagaaat gaaaaagata aatacattgt
    10861 ttataaagaa aaacttatag cttctgagtt atttaaaaat aataatgctt caataatgga
    10921 gagtaaagtt aactattgta taaaacaaaa catatctagt gaggagctaa aaaatatata
    10981 tgagtaaaaa tatatatgtt aaggaaacct atgaatggat aagagtaggt aatggtgaaa
    11041 atgagcttac agaaattgaa tatgaaaaat tgcttaaata tctagagaac aataatgatg
    11101 tattaaaaag taatatagta gatattaaat acaaaaaact cagatttata aactatgtag
    11161 gcattatatg ttttgaaaat gtaatacttg aaatacttcc taaattgtca ctaagtgata
    11221 atcttgtaaa agatagagag atactattac agatgctttc tatatgtaat aaaattccaa
    11281 ttataatgaa tgaaaaaata agattgagtt tgaaaaatta taatctctta aatttttttg
    11341 ttatgtattt cattgagagt atgcaaacac agatgaagag agggatttac tttgaatata
    11401 taaataaaat agagaattta aatgttatta ggggtaaaat attattaagt acatatgcaa
    11461 aagaaaaagg catttctcca atgaaaataa gatgtgaata tgatgagtat agtgaaaata
    11521 atttcttgaa tcaagttctt aaaaaagcat gtatatctat tttatgtaga ataaatgaca
    11581 attcaattca aggcaagatt aaaaaaattt taagttattt tcaaaatgta gatttaattt
    11641 atatagatag aaaaaaatt
//
LOCUS       DAEMAE010000117        11432 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEMAE010000117 DAEMAE010000000
VERSION     DAEMAE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.119"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(9..473)
                     /locus_tag="KPH93_002387"
     CDS             complement(9..473)
                     /locus_tag="KPH93_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689196.1"
                     /translation="MVHLVYCDNSGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVIGTSIPYNYEKSKF"
     gene            739..1677
                     /locus_tag="KPH93_002388"
     CDS             739..1677
                     /locus_tag="KPH93_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1689197.1"
                     /translation="MNKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKNWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            1805..2818
                     /locus_tag="KPH93_002389"
     CDS             1805..2818
                     /locus_tag="KPH93_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1689198.1"
                     /translation="MTKLSKKNMTYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMIFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNTVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            2824..3846
                     /locus_tag="KPH93_002390"
     CDS             2824..3846
                     /locus_tag="KPH93_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1689199.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALVGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            3861..4643
                     /locus_tag="KPH93_002391"
     CDS             3861..4643
                     /locus_tag="KPH93_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689200.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKNGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            4655..5995
                     /locus_tag="KPH93_002392"
     CDS             4655..5995
                     /locus_tag="KPH93_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1689201.1"
                     /translation="MENQEALRHEKIWILLFRYSTPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSMAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSISYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLSARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTHYFIR
                     GKSNLKLKKCNLKLDKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGININLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLVIVLPIQFNLDGVWLAQPIADFIAMIIVLLFLKREFKTSQV"
     gene            complement(6090..8369)
                     /locus_tag="KPH93_002393"
     CDS             complement(6090..8369)
                     /locus_tag="KPH93_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1689202.1"
                     /translation="MNFENTKNKKIIATLLLTLLIIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSIGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKVLQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFNGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDNNKLEEMKINLKKNKTGSMHYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAKFDLLLNKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDIISNYLEPEE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNILKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKTLEETSMDSFS"
     gene            complement(8712..9875)
                     /locus_tag="KPH93_002394"
     CDS             complement(8712..9875)
                     /locus_tag="KPH93_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1689203.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKDLASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVGKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEKMKDKFLKFVSDVEKR
                     AISMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIIPPNKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIVEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(9888..10163)
                     /locus_tag="KPH93_002395"
     CDS             complement(9888..10163)
                     /locus_tag="KPH93_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1689204.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEVIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(10184..11158)
                     /locus_tag="KPH93_002396"
     CDS             complement(10184..11158)
                     /locus_tag="KPH93_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG1689205.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQSLEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKAIFKTNKNIEVN"
BASE COUNT         3928 a         1598 c         1809 g         4097 t
ORIGIN      
        1 ttgatacttt aaaatttaga tttctcgtaa ttgtaaggta tactagtgcc aataactaca
       61 tcttcaattt tttcaacaat aagccaatcg tccatattcc cttgatggtc aaaatcaaga
      121 ggtgttattt cttcaacatt ttcaaattca actaagcata aacatttttt atgccaacga
      181 accttttgct tttcagataa atttaatttc ggttgatttt catttaaaat tttagaaatt
      241 tcatcttctg ttaatttaac atagttttct acttttttaa cagtagcttt tgcagatatt
      301 tttgcagttc ctttttccat aaaataaagt aactcatctt caaatactct gctatgtggt
      361 atttttctac cagcagcacc tctaataacc attgtttttg ttccagctaa gattttatct
      421 aagacctttt ctcccttttt tcctgaatta tcacaatata ctaaatgtac catgttttga
      481 accctcctta ctaaattcct tatctatagc attttatcat tgtttaactt aaaagtcatt
      541 gtatttatat ccaattttta aaaaaaatac tgtttaatat aagaaaacct tatctaagct
      601 atatataatt aaggttaatt aggaattttt atccttttac aaaataatat tgactaaaaa
      661 tagaaaaatg ctataataaa tatgtgctta tgataatcaa tatcacataa gtataataaa
      721 catgtaggag aaataatcat gaataaaata aaatcacttg caatatttat atctattata
      781 actacgttag tacttgttac agcatgttca gataagaata cagaagataa agataaatca
      841 gagacaagag ttgtacaatc tgttaaagga gaggttaaga taccatcaaa tcctaaaaaa
      901 atagttgata tatcaggaag tagtgaagaa ctattattag caggttataa gcctgttgca
      961 acagcaaatg tagactcata tgaaactgat aaattaccaa gttacataag agaagagctt
     1021 aaaggtgtaa agatagttgg gcactcaatg atggatacta tggatatgga agctatatta
     1081 gaagtaaatc ctgatttaat aataatgagt caaagacaag aaaaaatata tgaccaatta
     1141 aaagaaatag ctcctgtagt tatgatgaaa gattatgcaa atgactggag aagcaagtta
     1201 actgatgttt caaagttatt tgataaagaa gaagaagcta aaaattggct acagaagtat
     1261 gatgaaaaag ctacaaagtt aggtaaagaa gttatagaaa agaatggaga aaaaacttat
     1321 ttaccagtgt tggcaagttc aggtcaattt atggtattta gtgatggtgg aataggtact
     1381 ctaattaatg atgatatgaa attagctaga cctaagaata tgccaaaaca ggatggaata
     1441 acactaccaa tggtaagtat ggaaggttta acagatatag atgcagacca tatagttgta
     1501 attgctacag aatcagataa gaaagattta gaaaatagtg ctatctgggc tcaaataaga
     1561 gcagtaaaag atggtaatgt aacaatactt gatgctgctc catttttcag tcaatcatac
     1621 aatccaattg gaaaagagtt attattagaa tcagttaaaa atgaattaac aaaataattc
     1681 ttaacttagt aggtgtgaga taatgatatt catttttcac acctattttt aataaatctt
     1741 aaaattaaaa tacttaatag atggtattat acagacgatt aaaagggaaa aggaggtagg
     1801 atagttgact aagctgagta aaaaaaatat gacttatttg ttgatattag taagtatagt
     1861 tgtacttgta tttggaattg tattatccat aactattggg gcaaaagata tgaatctatc
     1921 aactgttata gatagcttga taaaaatgga agatggaata aatatgagaa tagtaaagga
     1981 tgttagactt ccaagggcaa tagcagctgc acttgttgga ggatttttag cagtttccgg
     2041 ggcaataatg caaggaataa cgagaaatcc tatagctgaa ccatctgtta taggaattac
     2101 acaaggagct acattcgcaa tttcgatatc actggtactt caaaaaaaac ttcctcaact
     2161 tatacatgga agttttagtg taatgatatt tgcatttatt ggagcaagta taagtggatt
     2221 atttatatat tttataagtt ctaaatcaag aggaagagtt aataatgtaa aattagctct
     2281 tgcaggagtt gctcttggta cattgctaat ttctttggca tcagcgatat ctatgtattt
     2341 taacctatct caacagttaa gtttctggat atcaggaggg cttgtaggag tgaagtggga
     2401 aggtataaaa ttgctattta tagctggtgg tattggattt attttagcta ttattatggc
     2461 tccaagaata actatattaa gtcttggaga agaagtggca ataggattag gacaaaaaac
     2521 taacactgtt agatttatat gtatagtact tgtaatatta atgactggag catctgtgtc
     2581 agtgtctggt aatattattt ttataggtct tatagttcct caaatagcta aaggaatagt
     2641 aggttcagat tataagtata taataccgag ttctttagta ttaggagctg tattgcttgt
     2701 atatacagat atattaagta ggatgataaa tcctccatat gagacaccag taggttctat
     2761 aacagcactt ataggagtgc caatatttat atatcttgtt agaaaggggg agaaataggt
     2821 gctatgaaaa atattataaa aataaattct aaaacattac ttgttatatc aattttaatg
     2881 gtgcttattt ttatcacttt tttattgagt ttaaatatgg gttcattttc tatagaacca
     2941 atggatgtta taaagacttt tttagggcaa ggtcagcgta atcatgaaat tgcaatattt
     3001 aaattaagat taccaagaat agtaatagca cttttggttg gttctgcatt atcaacagca
     3061 ggtgtaatac ttcaaggagt tacaaagaat gatttagctg attctggaat tttgggaata
     3121 aattcaggtg ctgcattatt tgtagtagtt tatatatacc ttatgaatgg taatgtatat
     3181 gaaggtatga gtaacttgac tgtatttaca atgcctattg ttgcacttgt aggagcttta
     3241 tttggagcat ttcttatata ttctcttgca tggaaaggtg gtataaactc gtcaagatta
     3301 ctacttattg gtataggtgt aaatatagct tttacttcaa ttttgacgat attccaattg
     3361 aaatttacaa cacaagaatt taatagggtt atggcatgga catcaggaag catttgggga
     3421 accagttgga aatacgtaat agctgtgctt ccatttatag tgatttttat ggggataact
     3481 atttataaat ctaaatatat agatgtttta aatttgggtg atgaagtatc tataagtctt
     3541 ggtgtagatg tagaaaaaca aagaagaaga cttataatat atgcagtaat tttatctggt
     3601 gtatcgacag cagtagcagg tagtatttca tttttaggtt taatagctcc acatataggt
     3661 agaaaaataa tagggccaaa gcataaaaga ttaataccag tatcagcatt gataggaaca
     3721 ttattacttt taatagcaga tactatatca agaaatcttc tagcacctat agaaatacca
     3781 gtgggtatag tagtttctat aataggagtg ccatacttta tttatttaat gttggtaaat
     3841 gattaggaaa gggtgttgtt atgaactgta taaatactaa gaatctaaat gtatcatatg
     3901 gaaatacaga tattataaaa aatttaaatt taaatatacc aaaaggaaaa ataactacta
     3961 tcataggggc taatggttgt ggaaaatcga caatacttaa aactataggt agaattatta
     4021 gccctaatag cggaaaaata tatataaatg gagaggatat atacaaaaag aagtctaaag
     4081 aaatagcaaa ggatatggct gtacttccac aaagccctca agctcctagt ggtcttaccg
     4141 tttcagaatt aatttcttat ggaagatttc ctcataaaag tggatttggt aattctagta
     4201 aagaagacag agagattgta aattggagtt tagaagttac tggaattgca gaatttaagg
     4261 atagaaatat ggaagattta tcaggaggac aaagacaaag ggcatggata gctatggctt
     4321 tggcacaaga gactgatata ttattactgg atgagcctac aacatattta gatttggcac
     4381 atcaattaga gatactaaaa ttattagatg aactaaataa aaaacaagga agaactattg
     4441 tcatggtaat acatgaactt aataatgcag caagatttgc tgaccacatg ataggcatta
     4501 agaatggtaa aatagtttgt gaaggtgatg catatgatgt tatgacgaaa gataatctaa
     4561 aagaattatt taatattgat gcagaaatag caacagaccc aagaacaaat aagccaattt
     4621 gtataactta tgacatggtg taaatggtga caacatggag aatcaagaag cattaagaca
     4681 tgaaaaaata tggattttat tatttaggta ttcgacacct gcaataatag caatgatggt
     4741 aacttcactt tataatgtag tagatagagc ttttattggt tctatggaag gtattggttc
     4801 tatggctatt gcaggtcttg gtgtaactat gccagtattt actctaataa tagcttttgg
     4861 aatgttagta tcagtaggtg ctagcacaag attgtctata aagttagggg aaagaaatag
     4921 agaagaagca gagaagatat taggtaatgc tttgacatta tctataatta tctctttaat
     4981 tataactata ttagggttag ttttcttaga agatatactt tttattttgg gtgcaagtaa
     5041 agattcaata tcttatgcaa aagactatat gagtgtcata ttgattggaa gtatatttaa
     5101 tttagttgca ttttctctta ataatgcaat tagggcagaa ggtaatccaa aattatcagc
     5161 aagaacaatg attgtaggat gcgtattaaa cttgatattg gaccctatat ttatatttgt
     5221 gtttaattta ggtataaaag gagcagctat agcaacagta ctctgtcaaa tagtagtatt
     5281 cttttgggta acacattatt ttataagagg aaagtccaat ttaaaactaa aaaaatgtaa
     5341 cttaaaattg gataaagata tagtaaagaa ggtatttgca ataggcatga caccttttgc
     5401 tatggaagta gctataagta taactcacat gtttacaaat aattctctaa aggtttatgg
     5461 tggagattta gctattgggg ctatgacagc acttacatct attttattga tgtttatgat
     5521 gcctgtattt ggattgaatc aaggtatgca aactataata tcatacaatt acggtgcaaa
     5581 acaatatgag cgtgcaaaga aaactttaat attatctatc atagtgtcag ttgtcatatt
     5641 gtcatttgga tttttggtag tacaagtttt cccagaagta tttgtaggca tatttaataa
     5701 agattcagat ttgatggata tagcagtaag aggtataaac ataaatctga ttacattgcc
     5761 tataatgggg atttctattg taggacctgt gtattttcag tgtataagta aagttaaaca
     5821 ttcaatgttt ttaactcttt taagacaatt tattttattt ataccattag ttattgtact
     5881 tccaattcaa tttaatttag atggtgtttg gttggcacag ccaattgcag attttattgc
     5941 tatgataatc gtattattat tcttaaaaag ggagtttaaa acaagccaag tataagaaat
     6001 ctagaaagta aaatgaaatt ataaaataaa aagacttgtc tttataaaat tattaaaatt
     6061 attttataga gacaagtttt ttattttatt taactgaaac tatccatact ggtttcttct
     6121 agtgtttttc caaaagtcat tttttcaaat atttcttgag gcaaaggttt tgaaaaaaca
     6181 tatccttgta tcatatcaca attaattttt ttaagataat ccatttgcaa gattgtttca
     6241 acaccttctg atatactatt catatttaat ttttttgcca aattaactat agattctatt
     6301 acatgatttt cagcaatatt atctgcatta ggtgagctaa aaaaagcacg gtcaagtttt
     6361 aatgtgtcaa cttttatttc ttttaatata ttgagtgaag agtatccact tccaaagtca
     6421 tctagtgaac attgaaaacc ttcttcgtgg atttggtcta ttatgttaat tagtttttgg
     6481 agattttcaa atacaagggt ttcagttaat tcaatttcta agagtttagc aggaatgttg
     6541 tatttattac gaagcattct gtatttttct aaaaaatcta tattatttaa attagcacgt
     6601 gaaaaattga ctgaaacagg tattggagtt tcaccattat ctatccattt tcttatcata
     6661 ctacatactt tttcaaaaac atagtggtcg agttttgtaa taaaaccgtt tttttcaaag
     6721 aaaggaataa actcgtctgg tggtataagt cctttatttg ggtcttgcca tcttactaat
     6781 gcttctgcac ctacaatagt attatcttta aggctgattt taggctgaag atatactata
     6841 aattcgttat tatttaatgc ttcctccatt ctattttcca tttctttttc tttaatcatc
     6901 tttaagcgtt ctaaatcact ataaaaaaca catgtataaa gtggatgatt tccaatgttt
     6961 cctttattat tttttcgtgc aacatttgca cggtcttgaa ttgaaactaa aggtagattt
     7021 gggtcatcaa ttttatatac acctatagat agggctataa aatatttaca ttcttcattt
     7081 ttattgaaac tattaatatc attagtgata ttttctatgt acttaagaat ttctttttga
     7141 ggaatagttt taataagtac attaaactgg tcattagaaa ctcgacaaag taattcttct
     7201 ggttctagat aattactaat gatatcatgt atgtacttta atgttttatc tcctgcttca
     7261 ctaccaaaag aatcattaat aagtttgaat ttctgaatat ttatagatac taatgaatag
     7321 ctttctgatg aagatgactg tataattttt ttagcttcca tatcaaaacg tgccttactg
     7381 taacctttag ttacagagtc tacataggcg atttttataa gttgtgctct gtttttttga
     7441 tttataaaca taatcaatat aataagtaat aaaaatagta taacaattaa aatattgatg
     7501 aagattgctt tatttagaag taaatcaaac tttgcacttg ctgtttttgt tggaacaatt
     7561 gaaaggaggt ataagttttc ttcttgtaat ggaagatagt gcattgcttt agaaatacca
     7621 tcatttaatg tataatgcat agaaccagtt ttgttctttt ttaagtttat tttcatttct
     7681 tcaagtttat tgttatcaac ctgttttcct tgatgtttaa tcatgtcaaa aaaatttact
     7741 acattttttg gagcattttt gtttgctgaa cttacaataa aatttccatc ttggtcgata
     7801 atttgggaaa atccttcacc attaaagctt tctacactta ataaatttct catagtatca
     7861 atagtattcc aagcagcaac agctccaaca attttatcgc cattgtatag tggtacagaa
     7921 tataaaattc catatgtacc agattgtgat ttaacaggag taatggtttc tttaccttga
     7981 aggacttttc tataaaagtc ttgtccttta acattacctg tatctttaag ggcacttaca
     8041 aaatttccat tagaatcgat aatacctaac caatcaaatt caaataattt taattttttt
     8101 gataagtatc tattgacttc tttttcacta tcattaatct caaaataagt atctcctata
     8161 gaatataatg tcctaaatat acttttacat ttcacattaa ttgtagtgga aatactttgt
     8221 gaaacttcta caagatatcg ttctgtttca ttatgtgtta ctttatttag attcataata
     8281 aaagttacac atgttaagga cacgattata agaagtgtca atagtaaggt agctattatt
     8341 tttttatttt ttgtgttttc aaagttcatt tttttatttc atcctccatc ctttatttgt
     8401 ttacaaataa gaattctctc agacatatta taccaaatat cttttcatac aaagatatta
     8461 aattttattt atttgacttt atatttaact ttatttaagc tttttagaga gtagataaga
     8521 tatgtttata gttttaaatc gtattttatg atgttttaag taaaagatgc taaaaaggaa
     8581 ctgttctcaa aataactttg atacagttcc ttttcagtat tgtaaatttt tataagatta
     8641 gaaaggactt ttagtttata catctatata agtattatat ttataaaaaa tgttttattg
     8701 tctattgcaa tctaaaatgt agatgaaatt ctgtaaatac caaattctct atgaccaaga
     8761 ctttcagctt tggtatgctt accattacat acttctacta tttcattaaa tatttcttga
     8821 cctatatctg ctattttagc accatctgtt ataattctac ctgcatttat atcgatattg
     8881 tctggcatct tgttgtatgt atcactattt ccagtgattt taataactgg tgaaataggg
     8941 cttcctgttg gagtacctct accagttgaa aagattacaa tttgtgcacc acctgctacc
     9001 ataccagtga tagaatctat gtcctgacct ggagtatcca taaaatataa tcctttttta
     9061 ttaggaggta taatatcagc atattcaaga gcacctttga atggcttatt tcctgcctta
     9121 tacatacatc caagtgattt ttcttcaatt gttgaaagtc caccagcttt attgcctgga
     9181 gtaggttgac cactacgcaa atcttccccc atagaaattg ctcttttttc tacatcactt
     9241 acaaatttaa gaaatttgtc cttcattttt tcatcttcaa aacgagtagc caataaatgt
     9301 tctgcaccaa ttacttctgt agtttcactt agtatgctac ttccaccttc atctacaaga
     9361 aggtttgatg caacgcctat tgaaggattt gaagctattc cacttgttgg gtcagaacca
     9421 ccacattcaa gtgctaatgt aatttcactc ataccaaatt caactcttac ttcttttgat
     9481 acttcacgag ccattttacc tactatttca gctcccttag aaactgtttt taatgttcca
     9541 ccacattctt gtataacaac gtaatctacc ttttttcctg ttgtagctat ttcagatgct
     9601 aagtcttttg cttgaatacc atcacatcca agaccaacta ctaaaactgc ggcaacatta
     9661 ggattttttc caaatcctat taatgtcttt gctgtaagct ctatatcaga gccaatttga
     9721 caacatccat gttgatgagg gatagctatt gctccaggca caagtgatgc tattcttgta
     9781 gctgtctcac ttgaacatac agatgttgga ataattaaaa catgattacg aataccaaac
     9841 tgaccatttt ctcttaaata acccattaat ttcattttaa cttcctccta ttttttatct
     9901 ccacgaccac gtgtactttc aatgttatgt acatgaacat agtcaccagt ttttatgtca
     9961 actgttgcaa ttcctataac ttcaccgtat ttgtatacaa catcaccttt tttaacatct
    10021 tctgtagcca tcttatgaaa gacaggtata ttatcattta ctatgtaaga ctttccatca
    10081 attgaaactt tttcaccttt aactaaaggt ctgacacatg taactagatt atctttttca
    10141 tttgcatgaa taattgcatt cattaatttt acctccttaa gtattaattt acttcaatat
    10201 ttttatttgt tttaaaaata gcttttttct ttttttctct tttatctaga taggaaacta
    10261 acattggaca acatattgct gtaacaataa tagcagcagc aacctgtact gttgaaacag
    10321 ctgctattgt agctagagta gggtctatag ctgctaaagc ttcaggagta ccagctgcat
    10381 ttccggctgt tgtacctgta gctgcaccta ctgctggatg ttctgcacgt ataagtttca
    10441 ttacaaagta cccacccata cctgttatta tagtaactcc taaaccaagt acaactccag
    10501 gcagtccagc agttataatt tgattaaagt ttaatgctgc tcctaaagga aaagcgaaga
    10561 aaggaattaa taatgttgta ccaggtgcaa gaaactctcg tatatcttca tcaaggttac
    10621 caagtataaa tccaagtagt attggtataa gtgctgcaaa tagtgctact ataggtacat
    10681 ttgcaagtcc agttactcca aaagcaagca ttgtgaaaaa aggaccatca tttagagata
    10741 agatagatat tgctccaaca tctgtggagt ctccatattc acctgcaaga gcagcaaata
    10801 gacctccatt tgaattagtc atggtagcaa ctatggcaag aggtgtaata ccaattatac
    10861 ctttaggtcc aataaattta ctaacaataa tacctaatac tgcaccaatt ataaattttg
    10921 ttgctgtaag tactatacct ttagtcaatg gcttaccagc ttgcctgata ttgatttgag
    10981 ctccattaca tagacaaaat agagcaagta tactcattgc accagtttta aataaggatg
    11041 ttgtgaatcc tccaatttca agagattgtg gacatactgt attaaatagt gctcccaata
    11101 acaagggaca gaccataagt cctcctggta tctttttcac tgattttaag atgtgcatct
    11161 agtatcactc cctttcattt ttatataggt atctaaaagc aattactatg ccaatataca
    11221 gagtttatat ctttagttat catgattatt tttaataatc gattgaaatg ctgtagaata
    11281 cttataatta aaagtatgat tgacgagaat ttcacttata aagattcttt ttatcatata
    11341 attgtatttt ttattgaaaa caaataaacg tcttatgtat taaaattaaa cataaaacgt
    11401 tttgatatat atagatatgt attattttta aa
//
LOCUS       DAEMAE010000118        11395 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEMAE010000118 DAEMAE010000000
VERSION     DAEMAE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.120"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..351
                     /locus_tag="KPH93_002397"
     CDS             <1..351
                     /locus_tag="KPH93_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689206.1"
                     /translation="IYLEGPSKISLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLTN
                     LYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNYI
                     FKEIETKEVIKLIF"
     gene            377..949
                     /gene="folE"
                     /locus_tag="KPH93_002398"
     CDS             377..949
                     /gene="folE"
                     /locus_tag="KPH93_002398"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG1689207.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            1015..1806
                     /gene="folP"
                     /locus_tag="KPH93_002399"
     CDS             1015..1806
                     /gene="folP"
                     /locus_tag="KPH93_002399"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG1689208.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSEEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRRDKNMASVIGKYDAEVCIMHNQDGTNYDKDIIASIKEFLKESIEMA
                     ISCGVKKEKIVLDPGIGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            1817..2182
                     /gene="folB"
                     /locus_tag="KPH93_002400"
     CDS             1817..2182
                     /gene="folB"
                     /locus_tag="KPH93_002400"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG1689209.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            2175..2681
                     /gene="folK"
                     /locus_tag="KPH93_002401"
     CDS             2175..2681
                     /gene="folK"
                     /locus_tag="KPH93_002401"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG1689210.1"
                     /translation="MNKVYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVVSTDEKLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            2662..3447
                     /gene="aroF"
                     /locus_tag="KPH93_002402"
     CDS             2662..3447
                     /gene="aroF"
                     /locus_tag="KPH93_002402"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG1689211.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQDKPIMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            3702..5492
                     /locus_tag="KPH93_002403"
     CDS             3702..5492
                     /locus_tag="KPH93_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG1689212.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            5510..6676
                     /gene="rpoD"
                     /locus_tag="KPH93_002404"
     CDS             5510..6676
                     /gene="rpoD"
                     /locus_tag="KPH93_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG1689213.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            6841..7530
                     /locus_tag="KPH93_002405"
     CDS             6841..7530
                     /locus_tag="KPH93_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG1689214.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VVIKKLENLIK"
     gene            7544..8641
                     /locus_tag="KPH93_002406"
     CDS             7544..8641
                     /locus_tag="KPH93_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG1689215.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSKKIKEKLNMKHIRVVGNLS
                     TNITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            8704..9276
                     /locus_tag="KPH93_002407"
     CDS             8704..9276
                     /locus_tag="KPH93_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1689216.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESIDVIKSGKELLDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGHPAYG
                     ILHIAGDNSDIRPSDDVKLGIRDYLKRLNI"
     gene            complement(9336..9803)
                     /locus_tag="KPH93_002408"
     CDS             complement(9336..9803)
                     /locus_tag="KPH93_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG1689217.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(10189..10665)
                     /locus_tag="KPH93_002409"
     CDS             complement(10189..10665)
                     /locus_tag="KPH93_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG1689218.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(10785..>11395)
                     /locus_tag="KPH93_002410"
     CDS             complement(10785..>11395)
                     /locus_tag="KPH93_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG1689219.1"
                     /translation="AFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMV
                     LLYITSIGSIKSRIKIDTNRIEESRIENIKYLNKIGYLFLILMIIMTTQFFTTLLSIK
                     TKLSTAMNITTLLVIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLM
                     VNKRFGIGWTINFGNPLGKILYIAIALLLIFSLFSLIKSLLL"
BASE COUNT         4596 a         1232 c         2072 g         3495 t
ORIGIN      
        1 atttacttag aaggtcctag taaaatttca ttagaggaaa agtcttttat aaagcaagat
       61 attggagcaa aaatatcaaa tttaaaaaat aaagaaaaat ttgatgtttt tgaatatcat
      121 tttttgacga atttatattt aaaagatata ataattgggt actttaatat aaatgataaa
      181 tgggtaccaa aagacaaaaa attgctaaaa gttttaaaaa aagaaaataa tgagcttttt
      241 gaactagtgt caaaagtaag tgaaaattat gactatcaaa gacttttaga tgtttataat
      301 tatattttta aagaaattga gacaaaagag gttataaaac taatcttcta gtcacaaatc
      361 aatgggggga gtttaaatga ataaagttga taaagaaaag atacaacatg cagtaagaga
      421 aatactagag gcaattgggg aagacccaga tagagaaggt ctaatcgaga ctccaaatag
      481 agttgctagg atgtatgaag agatattctc tggtcttagt gaagaaccaa gagaccattt
      541 aaaagttctt tttgcagatg aaaaacatga agaattagta ttggtaaaag atattccatt
      601 ttattcatgt tgtgagcatc atctagttcc tttctttgga aaagctcata ttgcttattt
      661 accaaaagga ggaagactta cagggctttc taaacttgct agagtaatag atacattggc
      721 aaaaagacca caattacaag aacgaatcac taaaaatgct gcagatataa taatggaaga
      781 attacaacct tatggtgtct tagtagtagt agaagctgaa catatgtgta tgacaatgag
      841 aggtgtaaaa aaaccaggtt caaaaactgt tacatcagca gtgagaggaa tatttgaaaa
      901 ggacattgcg tctagagccg aggctatgag tttgattact atgaagtagc aatttattat
      961 agatataatc atttattgag atataaaatt taatttaagg gagtgtcatt aattgtgttt
     1021 gattatggta aaagaactta tataatggga atattaaatg taactccaga tagtttttct
     1081 gatggaggag attttaataa cttggacata gcaatacaac atgcaaaaga tatggttgac
     1141 caaggtgctg atataataga cctaggagga gaatctacac gtccaggtca tagctatgta
     1201 gactcagaag aagaactaag aagagtaatc cctgtaataa aaaaacttaa acaagaattg
     1261 gatataccaa tatcaataga tacttataag gcagatgtcg cagaagaagc cttaaaatta
     1321 ggtgtcacta tggtaaatga tgtttggggt cttagaagag ataaaaacat ggcttctgta
     1381 ataggaaaat atgatgcaga agtgtgtata atgcataatc aagatgggac aaactatgac
     1441 aaagatataa tagcgtcaat aaaagaattt cttaaggaaa gtatagaaat ggcaataagt
     1501 tgtggagtaa aaaaagaaaa aatagtactt gacccaggaa taggatttgg aaaggatttt
     1561 gaacaaaata ttgaagtatt gagaagatta aatgaattaa aagacttagg gtatcctatt
     1621 ttacttggga catctagaaa atcagtaata ggaaaagtac ttccagttga gcctaaaaaa
     1681 agattagaag gtacaatagc tactactgtt cttggtataa gagatggcgt agatatagtt
     1741 agagtgcatg atgtttatga aaatttaatg gcagctagaa tgactgatgc aatatataga
     1801 aagtaggtat aatataatgg ataaaatatt acttagtaat ttaggattct atggatatca
     1861 tggtgttctt aaagaagaaa attttctagg tcagaaattc tttgtggaca tggaacttta
     1921 tattgattca agagaagcag gtcttagcga tgatataaat aaatctgtaa gttatgctga
     1981 agtttataat gttgttaaag atattactga aaataaacaa tttaatcttt tggaggctct
     2041 agcagaaaat attgctgaag aagtcttaaa taaatttata cttataaatg gagttatggt
     2101 cagagttaga aagcctgagg ctcctgtaaa tggtatttat gactattttg gcgttgagat
     2161 aaggagaaca agagatgaat aaggtttatt tgggaatagg tactaacatg ggtgatagat
     2221 ttgataattt atcaagagca tgtgaacttt tgaaaaatag tgattctata tataaagtaa
     2281 aagaatcaag tttatacgaa acaaaaccat ggggatatac agaacaagca gattttctaa
     2341 atatgtgtgt agaaatagaa acagagtttg aaccttatga gttgttagaa tattgtcaag
     2401 aaatagagag agaactacac agagagagaa tagttcattg gggaccaaga actatagatg
     2461 tagacatact attttttaat gatgtagttt ctactgatga aaaactaacg atacctcatc
     2521 caaggattca agacagagct ttcgttttga taccacttat ggatttaaat gaagaactta
     2581 taataaatga aaaaacaata aaagaacatt taaacctcct atctgctgaa gaaagagaag
     2641 aagtaaagga gcttgtaggt tatgaaagaa aacctattta ataataattc aaagatagag
     2701 ataaatacaa ataacaaaaa gataactgta aaaaaggata aacttataat agctggacca
     2761 tgtgcaatag agagttatga gcaacttttg gagactgcta aatttgtaaa aagtcaggga
     2821 gcaaatattt taagaggtgg agcgtacaag ccaagaacat cgccaaattc atttcaagga
     2881 ttaaaaaaag aaggtcttga aatattaaaa gcggttaaag acgaagttgg tatggctgtt
     2941 attacagaac ttatggatgt aagagatatg gacgaactgt atagtataag tgatataatt
     3001 caaattgggt caagaaatat gcaaaacttt actcttttaa gtgaagtggg aaaacaagat
     3061 aaacctatta tgttaaaaag aggaatagca tctacaatta ctgaatggat aggtgcttct
     3121 gaatatatag caatagaagg aaatagtaat attataatgt gcgagagagg tataagaacg
     3181 tacaatgatt atactagaaa tacattagat ttagctgcag tacccattat tcaaaaggaa
     3241 acaggtcttc ctgttgtggt agacccaagt catgccactg gagttagata tttggtaaaa
     3301 cctatgtcac tggcatcttt tgcatgtgga gcagatggaa taatggtaga agtacatcct
     3361 gacccagaaa atgctttgtc agatggtgct caatcccttt gctttaatga atttgaagat
     3421 ttaatgaaat caattaataa ttattaaaag aggcaagagc ctctttttta ttcgtaatcg
     3481 ttgctaaggt aaatttataa gttataattt gatagttaga ttatttaata taaataaaga
     3541 tttaatattg acagaattaa tataaaaaat aaaaattttt tgattaataa gaaggatatc
     3601 aataaaaaaa gtcgaattgt gtatattgag tgattgacaa atttagaata tagtgtagaa
     3661 tagaaatgtt aacacaaaaa attacaccag aaggtgataa tatgaatgat ataaaagaca
     3721 ttattgaaga aataaaagct agatgcgata tagcaagtat aatatctgaa tatatgaata
     3781 taaaacaatc tggagctaat tacaaaggat tatgtccatt tcatggcgaa aagacacctt
     3841 ctttttatat aaatacatca aaacagattt ataagtgttt tggttgtggt gaaggtggag
     3901 acgtaataaa ttttgtcatg aaaatggaaa atttggattt tatggatgct gtaaaaattt
     3961 tagcaaataa atgcggtata gaaattaaca ctaacatgaa tgaagaaact aggattaaaa
     4021 tagaaaaatc taagaaattt caagatattc atacggaagc agctagattt tatctttcaa
     4081 acttattggg aagcaaaaat cttgggtatg aatatttaag aataagagga ctagatgata
     4141 aaattattaa gaaatttggc ttaggatttt ctttggattc atggaattca cttatgaata
     4201 ctctaatcag taaaggatat aaaaaacaag acttattgga gtgcggtcta attgcaaaaa
     4261 atagagatgg aactaattgc tatgataaat ttagaaatag agttatgttt cctatattcg
     4321 attatagagg aaatataata ggatttgggg gaagagtatt agacgattct ttgccaaaat
     4381 acttaaattc tccagacaca ttaattttta ataaaaaaca gaacttatac ggattaaatt
     4441 ttgctagaaa aaatttagaa agtaaaacta tagtcttagt tgaaggatat atggatttaa
     4501 tttcgcttta tcagtatggt ataaaaaatg tagtagcaac ccttggaaca gccttgactg
     4561 aacaacaagg tattttaata aagagatatg cggacactgc tattatatct tatgattctg
     4621 atgaagcagg aataaaagcg actttaagag ctatagatat acttactaaa ttaggtatca
     4681 atgtaaaagt gttggaccta aaagatgcta aagacccaga tgaatttgta agaaaatacg
     4741 gacttagtga ttataaaaaa gctatggatg tttctactca ctacataaaa tataaaatag
     4801 atcatcttaa aaaagaattt aacattcaaa aagatgaaga acgagtgaag ttcgcaaaag
     4861 aagcttctaa aattataaaa caattgacaa gtcctgttga aattgatttt tatacaaagt
     4921 atttaagcaa tcaaatagat attaatgtcg agtctataaa gagagaagtc tatggtaaaa
     4981 attacaataa accatataat aacaaaaatc aaaagaaaat agaagaaaaa gtaattgaaa
     5041 aagtcgaagt cagacaagac ggaaaacaat tagttgaaga aactctaata aaaataatgt
     5101 tagaagacaa aaaaattaga gaaatagcgt tacttaaagt agaagaaagt gattttttat
     5161 tgaaagaaag taaagaaatt ttaaattaca tgattaaaaa tcaagaattg gacaaaataa
     5221 ctattgacaa attgaaaagt ttaaacatat ccgaagaata cttaaaagaa ttaaattcaa
     5281 tttctttgaa tagtataaac ttagagaaca caaaagaaat agaaggaata ataacaaata
     5341 tcagaaaaaa ctcattagaa gagcaaatta atagcttgtt gagagaacaa caagaattag
     5401 aaaataataa tgatatgaaa gaggtagatg gtagagttat ggaaattgct ttaaaaatag
     5461 ttgagataaa taaaatttta aagagcttgt agagaaagga ggtttgagtg tggaaaataa
     5521 atcgaataaa aaagagttaa aaaaggttac tgctaagaca ttaatagaaa aaggaaaaaa
     5581 gcaaggttct ctgacacttg cagagataat ggaagctttt tcggagactg aacttgataa
     5641 ggatcaagta gaaaatcttt atgagacttt aggaaatttg ggaatagaaa taacagaaac
     5701 aaaaaactat aaagctgata tagatttttc ggttgctgat gacgatttaa gtataggcca
     5761 cttagatgaa gatgcagagg caatttcaca tgatgactct tctgcaatag aaatagaaac
     5821 tgtggattta tctttaccaa aagggataag tatagatgac cctgtaagaa tgtacttaaa
     5881 agaaatagga aaaattcctc tacttaaacc acatgaagaa gtagaatttg ctagaaggat
     5941 gcacgaaggt gatgagatag caaaacaaag gttagttgaa gcaaacttaa gattagttgt
     6001 aagtatagca aaaagatatg taggaagagg tatgcttttc ttggatttaa tacaagaggg
     6061 aaatttaggt cttataaaag cagtcgaaaa atttgactat acaaaaggat ataagtttag
     6121 tacttatgca acatggtgga taagacaagc tataacacgt gctattgcag accaagctag
     6181 aactataaga ataccagttc atatggtaga gactataaat aagctaataa gagtatcaag
     6241 acaactactt caagaacttg gaagagatcc aaaaccagaa gaaattgcaa aagaaatgga
     6301 aatgacagaa gataaagtaa gagaaataat gaaaatagct caagaccctg tgtctttaga
     6361 aacaccaata ggagaagaag aagatagcca tttaggagat tttattccag atgatgatgc
     6421 tccagcccca gcagaggcag cagcatattc tttattaaaa gaacaaatag aagatgtact
     6481 tggttcgtta aatgatagag agcaaaaagt attaaagctt agatttggtc ttgaggatgg
     6541 tagagccaga actcttgagg aagttggaaa agagtttgat gtaactagag agagaataag
     6601 acaaatagaa gcaaaagcac taagaaaact aagacatcca agtagatcaa aaaaacttag
     6661 agattattta gactaaaaag atacatcatt aatgtttttg tgataaatgc catgagataa
     6721 gtgatatctc atggctattt tgtagtaaag atttaatttt atttttgtag ttatttcaat
     6781 ctaatatcta taacttggat taaatcctat aaataaaggg aattgaaagg agaattatat
     6841 ttgaaattaa cagatagatt attgaaaata gcatcattag tgtcagatgg taaaaaaata
     6901 gctgatatag gaacggacca cggatacata ccagtatatt tgttaaaaga gggaaaagta
     6961 ccatttgcag tgctagcgga tgttaataaa ggacctcttg acaatgcaca taaagaagtc
     7021 atacagaaca atcttttaga taaagttgat ttacgattgg gttctggtat agagatactt
     7081 gaaatagggg aagtagaaga agtgataatt gccggtatgg gtggaatctt gataagtgag
     7141 cttctcgagg ctaaaaaaga ggtagcacat aatgtagaaa aattaatatt acagccaatg
     7201 caagcacaag aagaacttag atattacctt ttaaacaacg gttatgaaat tttagaagaa
     7261 gttttagtga gagaagattt tagaatatat gagataattg ttgccaaata tacaggtaaa
     7321 aatactataa tagaagatga gatatattat gaggttggaa taaagctttt agaaaacaaa
     7381 gattctcttt ttaatgattt tatagaaaaa aagataaaga catatagttc tatagttaat
     7441 aaattagaag gaaaaaatgg ggaagctata gataagaaga gaaaggaaag cgaagttgta
     7501 ataaaaaaac ttgaaaattt aataaaataa ataggaggta tatatgctat taaagagtct
     7561 aactagaaaa attgaaaaaa agtatcctct aaacttagct gaggattggg ataatgtagg
     7621 tcttatagtt ggagattttg atatggatgt aaaaaaggta ttggtatctt tagaagctaa
     7681 tgaagatgtt attgatgaag ctatatctaa aaatatagac ttaattgtga cacatcatcc
     7741 ctttatattt gggaaaatta ataaaattaa tagtggagat ttaaaaggca gacttatcca
     7801 aaaacttatt aaaaatgata tatcacttta ttctatgcat acgaattttg atatagcatt
     7861 tgatggtcta aatgactatt ttatggaaat tatggaattt ggtaattcaa aggttttaga
     7921 tataactaaa agcgaaaatt tatataaatt agcagtatac gttccacata actattcaga
     7981 tgaacttaga aaggttttaa gtaattcagg agcaggtcat ataggaaatt atagtgattg
     8041 tacgttttca atagaaggtg aaggacaatt taagccatta gaaggctcta atccgtttct
     8101 tggaagtgta aatgatatag aaactgtaaa tgaagttaaa attgaaactg tagtaccaca
     8161 gaagctttta ggtggggtaa taagttctat gttagatgca catccttatg aagaagttgc
     8221 atatgatttg tacaagttag aaaataaagg tgagatattt ggactaggta gaataagtaa
     8281 acttgataaa agtatgacat tagaaagtct ttcaaagaaa ataaaagaga agttaaatat
     8341 gaaacacatc agagtagttg gaaaccttag tacaaacata acgaaagtag ccgttgtaac
     8401 aggagctggt tcagaatttg taaaaaaagc taagagacaa ggtgcagaag tactaattac
     8461 aggtgatgta aagtatcatg aggcacaaga tgcacttgat attggtatgt gtatagtgga
     8521 ttgtggtcat tttgatacag aagatatatt taaaaatgtt atgaagagat ttttagatga
     8581 atttagtgaa attgaagtaa taaaaagtaa tgtatatttg aatccattta gtactattta
     8641 gtagtgtttg attataaaaa tactatataa aaaataaatt tgagtttaat tagggggata
     8701 aggatgaaag tagcagtaat ttttcatagt gtttgtggga gtacatattt attggctaga
     8761 gaatataaag aagtgcttga agaaatgaat atcgaggtag atatctttag agtaagtgat
     8821 gaagttgcaa aaacacttcc tcaatattat ttgataaatt ctaaagaata caaagatgaa
     8881 tttgaaagta tagatgtaat taaatctgga aaagaactat tagattatga tgctatattt
     8941 atgggctcac caacatatta tggaaatgta tctggaccaa tgaagatgtt tatggatagt
     9001 ttttctgata tttgggtagg tgctcctttg agtggtaaaa tatttggatg ttttgctaca
     9061 gcaggttcac aacatggagg aggagaacta gctttgcaag ctatgaatat atttgcccaa
     9121 catatgggaa tgactctctt gtcagttcca tgttcagtaa gaggaggtca tccagcgtat
     9181 gggatacttc atatagcagg agataattct gatataagac caagtgatga tgtcaagcta
     9241 ggtataagag attacctaaa aaggcttaat atataagaat atcaaataaa aatacctaaa
     9301 agtggctgta tatactttta ggtattttta tttgattata agcgtgaagc tttaccagaa
     9361 atatggtcaa tttctatttt aacaacagtt gttaagttca tatccttctc tatgtacttc
     9421 atgccttctt caaagaatcc tttagaatat ttttttatta tttctacaag agcattcttc
     9481 ttttcttcat tttctacagt gcaagctttt ccaaaaacta tagcactaga ataagtagta
     9541 ctaaatttat ctggaataac actttcatta gcaactacta aaaaagaaac tttgttgttt
     9601 ttcgatatat tatctaactt atgaccattt cttgcacaat gaaagtaaat agaatcatta
     9661 aagtatacat aatttacagc aactccataa ggataaccat tttcagatat agttgaaaaa
     9721 gtaccaaatt caccattttt taatacttca acagtatctt cttttgtcat ttctcttttc
     9781 ttcaatctca tttctttaaa cataatatca tcctttctaa ttttgatgct taaattaaat
     9841 tttagaacat ttataaagat tatgcaatgc tttttacaaa ttttaagaaa aaaatatagt
     9901 ttaagataaa aggtagtgaa agagaagtta tttggagatt tatttatgat attattgtta
     9961 aagtagtaca aacaatacta ataaatttaa tagttggttt cttgttattt aaggtagtaa
    10021 gcatgtctat aagaagggga agttatcgag ggtagaagtt gttatttata acgatattat
    10081 aaaaaaaata ttattatata aaataataaa tagtaaagag gtatcaagtt taatataata
    10141 cttaatacct cttcaaaatt aaattataaa agtgttatgg gaattgtcct atacatggta
    10201 aggagataat aaagaatgta tctttaaagt ctcaactaaa cagcaaaatt tatccaataa
    10261 acatacaata aatgcttctt tgtatctagg tggcttaaca gtaagtggcc acatatgttt
    10321 taaaattatg tccgtttctt tttcatttat ttgaaaaaat aaagttgcat tttttaatgc
    10381 ttctctaggg tgagtaaatc catgtaatcc ttttctatca cctttgtaat gccaatcata
    10441 aagaaaaaag tcgtgtagta aagctcctct aataacactt tgaatatcaa cacttaagtg
    10501 caatttttta cataatagat aactatagta tgctactgac aaactatgtt caagacatga
    10561 aacattgcca tgatgaggaa atgttctcat aagttgaact ttttcagaag aaagtatctc
    10621 agaagcacat tcattaaaat atttgatttc agcttttgac aacattatga aaatctccat
    10681 tcttagtaat atataaatac tatacttcaa cgataatatg aaatatttct tatcctacaa
    10741 aatataatac ccaataatac aagtaagata ccttttatgg tattttaaag taagagagat
    10801 tttattaggc taaacaatga aaaaattaaa agtaaagcta tagcaatata tagtattttt
    10861 ccaagaggat tgccaaaatt tatagtccat ccaataccaa accttttatt aaccatcaat
    10921 gatgggtcat ttggattatt atacatgatg cctgctatcc aatacttctc atcttcttct
    10981 ggagaataag atgagttgaa ttttaaattt ggggaattaa tatatgttgc tattaaataa
    11041 attataacta aaagcgttgt aatattcata gcagttgata gttttgtttt tattgataaa
    11101 agagtagtaa aaaactgtgt tgtcattata atcatcaata ttaaaaacaa ataaccaatt
    11161 ttatttaaat atttaatgtt ttcaattcta ctttcttcaa ttctatttgt atctatctta
    11221 atccttgact ttatggagcc aatagaagtt atatacaata aaaccattaa acaaaagtca
    11281 gtaccgatta atttaaatac attaagaaat gatttatcta taaaaacatc aggagaccca
    11341 tttatgttcc aatgagtggc aattgagtct ggtaatttat tataattaaa tgcta
//
LOCUS       DAEMAE010000119        11358 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEMAE010000119 DAEMAE010000000
VERSION     DAEMAE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.121"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(40..2181)
                     /locus_tag="KPH93_002411"
     CDS             complement(40..2181)
                     /locus_tag="KPH93_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1689220.1"
                     /translation="MLLSIVMMVKNEENILEKTLKSLSALRNSIESELIIVDTGSTDN
                     TIRIAKQYTEKVYFHNWNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFES
                     GLNKKFNSASVRLKNLYSFDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNI
                     ADFEHYGYLFEDEEIRINKVKRNEKLLLEELKEDEKSPYTNYQIGKNLIILGKYQEAL
                     DYLEKSYKLYSKLEIVPGYLITNLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYY
                     KLEKYDLVISEYDNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYREIPNSKVERDSF
                     YKSMEDFINNIKDDEKELIYKSLCDIEGNYGLLNKIRINKHVSLDTCRKILNEEKDIV
                     YASVIKIAFEEKVDLLDIFYNMEYVWIEKYLRYILALERSFNVSIYKYIISCPNTSEI
                     EKIRVYKVISKVLLESANLSDKKYKELFYIYIMYSYKYIKYIYSNLNDCELIKFVCND
                     IDKFTLEFKHLLDGKRKINNPNEKLEYLYNMRILLNEYPFYNRIIKIFISELEESITE
                     SKEFKLLKDNFIIRIENMIENGKINDAKLLIEDYSKTFADDVKILNIKAILFMCEDKF
                     EEADFALKKALSLDLENEDTIYNIEYLKNLK"
     gene            complement(2275..3147)
                     /gene="fliC"
                     /locus_tag="KPH93_002412"
     CDS             complement(2275..3147)
                     /gene="fliC"
                     /locus_tag="KPH93_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBG1689221.1"
                     /translation="MRVNTNVSALVANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILLRMRTLSLQSANEIN
                     NTEEREKIADELAQLKDEIERISSSTEFNGKKLLDGTSTTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTTSIGSMKAGGTSGTDAAKTMVSSLDVALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(3248..3583)
                     /locus_tag="KPH93_002413"
                     /pseudo
     CDS             complement(3248..3583)
                     /locus_tag="KPH93_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
     gene            complement(3586..5109)
                     /gene="fliD"
                     /locus_tag="KPH93_002414"
     CDS             complement(3586..5109)
                     /gene="fliD"
                     /locus_tag="KPH93_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG1689222.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSEIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKALAGYSSNYDDKGMFEKLKKIVFEYSGSSASKFAKKAGIEKTSSA
                     SQNVYSKQIAEQERNINSLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(5137..5487)
                     /gene="fliS"
                     /locus_tag="KPH93_002415"
     CDS             complement(5137..5487)
                     /gene="fliS"
                     /locus_tag="KPH93_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG1689223.1"
                     /translation="MELDLNELNGLSKEELLLMLVDGTVKYINISKVALLNRDYLKAH
                     NELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPIA
                     EQIRDTWHEVYNKL"
     gene            complement(5487..5882)
                     /gene="fliS"
                     /locus_tag="KPH93_002416"
     CDS             complement(5487..5882)
                     /gene="fliS"
                     /locus_tag="KPH93_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG1689224.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEEIKDTWHEADRKSREERSRYK"
     gene            complement(5904..6116)
                     /gene="csrA"
                     /locus_tag="KPH93_002417"
     CDS             complement(5904..6116)
                     /gene="csrA"
                     /locus_tag="KPH93_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG1689225.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            complement(6110..6502)
                     /locus_tag="KPH93_002418"
     CDS             complement(6110..6502)
                     /locus_tag="KPH93_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG1689226.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDVLKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCGMQIILQDEKYSIWHPLLRGDGGC"
     gene            complement(6518..7450)
                     /gene="flgL"
                     /locus_tag="KPH93_002419"
     CDS             complement(6518..7450)
                     /gene="flgL"
                     /locus_tag="KPH93_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG1689227.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     ASLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(7469..8779)
                     /gene="flgK"
                     /locus_tag="KPH93_002420"
     CDS             complement(7469..8779)
                     /gene="flgK"
                     /locus_tag="KPH93_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG1689228.1"
                     /translation="MAGLFSILNTANSGMSAHQKSIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEGLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLEINELKNVDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(8797..9213)
                     /locus_tag="KPH93_002421"
     CDS             complement(8797..9213)
                     /locus_tag="KPH93_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG1689229.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQILGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(9218..9493)
                     /gene="flgM"
                     /locus_tag="KPH93_002422"
     CDS             complement(9218..9493)
                     /gene="flgM"
                     /locus_tag="KPH93_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG1689230.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEATRNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            complement(9644..9958)
                     /locus_tag="KPH93_002423"
     CDS             complement(9644..9958)
                     /locus_tag="KPH93_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG1689231.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIEENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     IN"
     gene            complement(9982..10410)
                     /locus_tag="KPH93_002424"
     CDS             complement(9982..10410)
                     /locus_tag="KPH93_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG1689232.1"
                     /translation="MGINIDKLNISPSRSIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTNKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(10419..11087)
                     /locus_tag="KPH93_002425"
     CDS             complement(10419..11087)
                     /locus_tag="KPH93_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBG1689233.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKSRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
BASE COUNT         3384 a         1993 c         1114 g         4867 t
ORIGIN      
        1 tatttatatt attattttct aaataaccta gttttgaatt tattttaaat tttttaagta
       61 ctctatatta tatatagtat cctcattttc taagtctagt gataatgcct tttttaatgc
      121 aaaatcagct tcttcaaatt tatcttcaca cataaacaaa attgccttta tatttaaaat
      181 ctttacatca tctgcaaatg tttttgagta gtcttcaatc aataactttg catcatttat
      241 ttttccattc tcaatcatat tttctatacg aataataaag ttatctttta ataatttaaa
      301 ttctttactt tctgttatac tttcttctaa ttcacttata aagattttta ttattctatt
      361 gtaaaaaggg tattcattta ataatattct catattatat agatattcta atttttcatt
      421 aggattattt atcttccttt taccatcaag tagatgctta aattctaaag tgaatttatc
      481 tatatcatta caaacaaact ttattaattc acaatcattc aaattgctat atatgtattt
      541 tatgtattta taagagtaca ttatataaat ataaaaaagt tctttgtatt ttttatcact
      601 taaattagca ctttctaaaa gaactttaga tataactttg tacactctta tcttttctat
      661 ttctgatgta ttaggacaac taattatata tttatatatt gatacattaa aacttctttc
      721 taatgctaaa atatacctta agtatttctc tatccaaaca tattccatat tataaaaaat
      781 atctaataag tcaacttttt cctcaaaagc tatttttata acacttgcat aaactatgtc
      841 tttttcttca tttagtattt ttctacatgt atctaatgat acatgtttgt ttattcttat
      901 tttattcaaa agaccataat taccttcaat atcacaaaga cttttatata tgagctcttt
      961 ctcatcatct tttatattat ttataaagtc ttccatactt ttataaaaac tatctctctc
     1021 aaccttcgaa tttggaatct ctctataata gttttttatg tcttcaaatc tatttaattt
     1081 atataaggac ataaataagc taaagtacac attcttcttt ttttccacat cttcaatatt
     1141 atcatactct gaaattacta aatcatattt ctcaagctta taataaattt ttatcaatgt
     1201 aattatagct tcgtctctaa atcctacagc atcagtatct gaaagcaaac tatttgcttg
     1261 cgtactcatt tcataattat ctagcaaata aatatatctt ttatatgaat ctatactatt
     1321 ttcatacttc cctaaatcaa cttgtgcttg tgcaatatga taatatatat caatattatt
     1381 tctgtcctta ttaatatatt ttaagcataa tttttcacac tttttagcct ttcctaagta
     1441 taaatatact tttgcaaggt ttgttatcaa gtatccaggt actatttcta attttgagta
     1501 taatttataa gacttttcta gataatctaa agcttcttga tattttccta atataataag
     1561 atttttccct atttgataat ttgtatatgg agatttctcg tcttctttta attcctcaag
     1621 caacagtttt tcatttcttt ttactttatt tattcttatc tcttcatctt caaacaagta
     1681 tccataatgt tcaaaatcag caatattatt aaatatagga tttttataca ttggttgttc
     1741 atgaactttc ccacagtatt taaaatcaag atttttgaaa aatctcaaca ctgaacaata
     1801 tccatataat tttttatcaa atgaataaag attttttagt cgtactgagg cagagttaaa
     1861 ttttttatta agaccacttt caaaaaactt tataatattt gaataatcaa ttaatacttc
     1921 atctgcatct aatataagta accattcgcc cttagcgtag gatatagata tatttctcat
     1981 actagaaaaa tcatcattcc aattatgaaa atataccttt tctgtatatt gttttgctat
     2041 tcttatagta ttatctgtag aacctgtatc aactattata agttcactct ctattgaatt
     2101 tctcaatgct gataacgatt ttaaagtttt ttcaagtata ttttcttcgt ttttgaccat
     2161 cataactata ctaagtaaca tacatatttt cctttcaaaa ctatatttct aaaaaaagaa
     2221 aagaataaag caaagcctta tcctttcttt tttattcaat atactaaatt tattctatcc
     2281 taataattgt aaaactcctt gtggttgttg attagcttga gcaagcattg attgtgatgc
     2341 ttgaactaat atattcattt tagataagtt aaccatttct gaagcaacat ctgtatctct
     2401 tattcttgat tctgctgctg taacattctc taaagtatta tttaaattgt tttgagttga
     2461 ttctagtctg ttttgttgtg ctcctaattt tgctcttgat gagtttaagc tcttaagagc
     2521 tacatctaag ctagatacca tagttttagc tgcatctgtt cctgaagttc cacctgcctt
     2581 catacttcct attgaagtag ttgttatacc agctgaagcc attatacttg cagtatttac
     2641 taattgaatt tttatctcat tgttattatt acttgtacct gaaacattag ttccataact
     2701 tgctccaact tgtaatctta ttgttgtaga agttccatca agtagcttct taccgttaaa
     2761 ctctgttgaa cttgatattc tttcaatctc atcttttaat tgtgctaact catcagctat
     2821 cttttctctt tcttcagtat tattaatttc atttgcagat tgtaatgata aagttctcat
     2881 tcttagtagt atatttccag tttcttctaa cgaaccttct gctgtttgta caacagatat
     2941 accatcttga acgtttcttc ctgcttggtc taaacctttt atttgagctc tcattttctc
     3001 agatattgca agtccagcag catcatcagc agctctcttg attctaaccc cagaagataa
     3061 cttctccata gacttacttt gtccatttac gtttcttccc atttgattgt ttgctaccaa
     3121 agcacttaca tttgtattaa ctctcattat tttaccctcc ttagtatagt tgacatcctt
     3181 gtcatttcta cttttaactt ttgtattaac tatccattta tattatcgga caagttaatc
     3241 tataacttta tactttttta ttaaaaatat ttactttttc tctatttaaa ctataataca
     3301 ttgtgtttac ctgtttggta agcttgtact catgtaattc tttttttact tctgataatt
     3361 gttcttgcaa agcatctctt atcttagcat caatttcaaa tagttccata ctatcataaa
     3421 gttgtataaa ttcactttta tcaacttcac ttatactatt tataatgttt tgtctctcat
     3481 ctaattaaaa acttatattt ttatactcca ttttttcaat aaaatttata atttttaaag
     3541 atatatcttt atataaattt attttttctt ctaacttgtc catgattaat taccttgtgc
     3601 ttgtgagaaa tagttcattt gtgaagaata ttgatttaac aaagactcta aagctgaata
     3661 tttagcataa agtcttttct ctttatcatt cattttttca actaaactat ttatatttct
     3721 ttcttgctct gcaatttgtt ttgaatatac gttttgacta gcagaagaag tcttttctat
     3781 acccgctttt ttagcaaatt tagaagctga acttccagaa tattcaaata caattttttt
     3841 taatttttca aacattcctt tatcatcata atttgaagaa tatccagcta aagctttata
     3901 taccttgtca ctatcatcta taagagcctt agaaaaagta tctgcattaa atgatatttg
     3961 tccttttttg ttataattct catgctcact aatacccagt tttcttaaag caataatagt
     4021 atctgcatct ccaaaaaaca tagactgaat atcttcaaca aaagctctta attcatcatc
     4081 atttctaagt attccttcct ttgccttctt ttcccacttt tctatttctt cagttgtcat
     4141 atcctctttt tgttcatctg taagaggtgg ataatctttt gattttttag tagtaactaa
     4201 tccataaacc ttatccatca atgcattata atcatctaca aatgctttca tatttttagt
     4261 agcttcttca gtgtcagtaa cagaaaccag tttagcactt ccttttgaat ttacattata
     4321 atcaatgtta tctattttaa atgaattctg ttctttactt atgtgtgtta ctcttccttg
     4381 gtcatcagtt acatcagctt ctaaattttt tccatatact actgcagatt tagttccact
     4441 agcatcagtt gtaaatgtaa taccattatc actggcaatt tgtccagcta aactatcatt
     4501 tccaattact tttaaatcaa tctctgatga tgaaccagtc tgttttcttg atataatcaa
     4561 ttcacctgtc atctcgctat aagaagctct tatatcatta tctgcacaaa gggagtttat
     4621 cttacttaca atagtacttg tagtatcttg ttcagatatt ggtatattta caccacctat
     4681 atttaaagaa cttgctccac tagcatttgg aggaaactgt tgaacaatag ggtcacttga
     4741 atttaactta atagttactt ttgctggttc agccatttga gaaactgcaa attgataatt
     4801 tattttttct gcaccagctg agccttttgc tactataata ctttcatcag aactagttat
     4861 tcttgtagca gaatatgctt ttttatttgt tatactatta ggagagtctc catttagata
     4921 cttgtcataa agattctttg actcttttat aatatccctg tatatttctt gtttccattt
     4981 aactatttgt tgatcttgtt ttgctttatt aactttttcc ttatctctta tcatactagc
     5041 ttctattata ccttccatat caaaatttcc tgaaaggcct gtaactctta ctggacttat
     5101 acttgacata tgtttatccc cccatcatga ttatagttat agtttattat atacttcatg
     5161 ccatgtatct cttatttgtt ctgctattgg taatatatca tcaattattt taatatcttt
     5221 atttatatca gcctttacta attcatgctt tatgaattca taaactttat acatatcctt
     5281 tgcccattgt cctacacttt ggtctaatgt gaccattaat tctgtaaata tattttgaac
     5341 tctaacaagt tcattgtgtg cttttaaata gtctctattt aaaagtgcga ctttagatat
     5401 attaatatac ttgactgtac catctacaag catcaatagt aattcttctt ttgataaccc
     5461 atttagttca tttaaatcta actccatcat ttatacctac ttctttcttc tctacttttt
     5521 ctatcagcct catgccatgt atctttaatt tcttctatta caggtaatac attatctatt
     5581 atttgtatat cttttctgat atttgctctt gaaagttcat atcttacaaa atcataaact
     5641 ctatacatat ctttcgccca ttgacctgca ttttggtcta atgtaaccat tagttctgta
     5701 aatatatctt gaactcttat aagctctcta tgtgcttttc gtgtattttt atctataata
     5761 gctcctctag ctatttttgt atattttact gcaccatcaa caagcataag caataattgt
     5821 tcttttgatg ccataaaaat agcattttgc ttataagaat tataaggatt ttctccgtac
     5881 atatattact tcctccaagt gttttatttt aatgatttta agatttttat atttttattt
     5941 gtggctttta tattttcatt ttttactttt tcatatattt cttttcttaa gatacttata
     6001 ttattgggag cagatatggc taattttatg ttatttccat ccactcctac aacttttact
     6061 tctatgttat ctcctataag tactgcttca tctttttttc tcgaaattac tagcatccac
     6121 catcacctct caataatgga tgccatattg aatatttttc atcctgtaaa attatttgca
     6181 ttccgcaatt atttcttaca tttattacta taggagcttt catgttcaca gttgattctt
     6241 caagtgtttg accaacagtg attatattaa gcaacataat atcatttgga gattttactt
     6301 caagtttttc tattgtttct tgatttagga ctatttcata ttccttaata atttcaaatg
     6361 gtgatacaac aataaatcct atacaatctt cttcagtaga attaaattct tttaaaacat
     6421 cttcattttc tatatctaaa agctcatact cttttaagtt ttcaaatcct agtattcctt
     6481 tttttaatgt aatcttcatc atatctgcgt tattttacta tatataatct aatatagaca
     6541 cattaaataa ctttcctcct gtttgtagtg aagcttgata agttaattct gcagccttta
     6601 attgaacaag cttttcccca taatctattt catcattttg cgataaaaca tcctttaact
     6661 gtatcaaacc ttcatcattt ttttctttca tggcatctac cgtgtttgcc tttgtaccat
     6721 atatagacct aacatttact gtattatcta ttagattctc catgtcacct aaacaagttg
     6781 ttgtcaactc ttttatattt gcatcacttg gatttgccag tttctttgat atattattta
     6841 acttttcaaa tggatcagtt tttgttggat caaacccaag actactcaac ttaacattat
     6901 aatctacact gattccttga gctacatcta cacttaaaga atcatttaat ttattaagat
     6961 ttaagctagc attaaacttt aaacttactg agccatcagc attctctaca cactcaacag
     7021 gtggtgtgcc tgtatttgaa cctgaaaata tgtacttacc ttcatgtgtt gcatttaaag
     7081 catttgctat ttctttcatt ttttcattgg ttgaattact aatagttttt atttcgtcat
     7141 ctgaatatga accattccca cttgatacca aacctttctt tatatctgct aataaattac
     7201 caagttggtc taatgaagta tcagttgttt ctaaccaatc tgctgtatct ttacaatttt
     7261 caatataagt ttccatagtt tttatttcac tttttgaatt caatatcttt attgccttat
     7321 atggattatc tgatagttta tttatttctt tcgttgtgtt gatttgtcta ttgaccttgt
     7381 ctaatctttg caaattatct tgcaatgaat ttagataact actggacatc atacctgtac
     7441 taactctcat tataaattcc tccttttatt aaattatccc atttattaca acatccaata
     7501 gttgtactgc tgttgacata acttttgcac tagcttgata ggaacgttgt actttcatga
     7561 gttctgtcat ttcttcatct aaagatacac ctgaaacact cattcgtgaa ttatcaacat
     7621 tatttataat tttacgttga ttcacttcat cttgattaac tttttgagaa tctaaaccta
     7681 ccgattctgc aaaagccttg tagtggtcac ttattgtgct tacttttcca ccagctaccc
     7741 caatatctat tttttcatcc tttaacttag caatctttga agcttcttca gtagtaagct
     7801 tcaattcatt tgaattactt tttattgctg gatttacttt tataatatct tcaccagcta
     7861 catttgaggt tataaaaaaa tctcttacag ttattccagc agatgtatta taaacatcat
     7921 ttatctgttt tgctaatcca tcagatacat cttttaagtc gcttttatat gtatcaattt
     7981 taccatgcat ttcaaacaag ccttgtaaag ttcctgatat acctgttttc caagttccat
     8041 ctacattttt caactcatta atttctaatt taccatctga aagcatattt ttaacatcgc
     8101 tattttcaag gtctaactta aaactcaagt tatccaatat tctatctctt tcatcaagca
     8161 aatcatttgg tgtagagcca gaaccagaga taatatcaat agatttattt aagccttcaa
     8221 gactttttaa catgtcattt atttgtttta tctcagtatc tatattggaa tctatatctc
     8281 cttttaactt atcaagcttc ttataacttt cgcttattaa agtcgctaaa ttagatgcat
     8341 tttctaccac cagacttttt ttactatcac tatttatatc ctttgataat gactcccatc
     8401 ctttaaaaaa atcatctata cctgcagata taccatagtc tgatggttca tttagtatag
     8461 tttctatagt attataataa tcatattttg aaactgtatt cccatactta tgagcttctg
     8521 ctctaaactg gtaatcataa aaactatttc tagctcttgt tacatctgtt acatgtactc
     8581 cagtacctaa ttgtcccctt ccaattgaat tgctcaaact aggcatatac attggactat
     8641 ttgtagcaaa ctcggtcctt tgcctagaat aaccatctgt atctaggtta cttatattat
     8701 gagaaattgt ctgtattgat ttttggtgcg cactcattcc tgaattagct gtatttaaaa
     8761 tggaaaataa tcctgccata tttctcctcc caatatctat tttctaatgt ttccataaga
     8821 attataagtt gtagtttgtt tatcaactgg ttttatgaaa ttaatcattt ttttcgtgaa
     8881 aataagttct ttctttatta aagaatcatt tgattcttgt tgatttttag ctaaagcaag
     8941 aatatgcttt aagtctatat atatttcttt taaatattca tcattactgt tttcaacaac
     9001 ctcacttatt gaaacatcat cccctaaaat ttgtcttctt tttatttcta tagatgcaag
     9061 ttctctacta atatcctcta gtttaggatt caacctattt aaattttcta agtctttttc
     9121 aattatataa tcaaattgtt cttttagtaa ttccatcata ttactaatta tctttttttc
     9181 ttcatatatt atgactttta aatctgtaat catgacttta tcctcgcatc tcctctatca
     9241 tttttttagc aatttcttct ggtttaattt tgtaagttcc attttctaat gcacttttta
     9301 tttcttctat ttttttattt ctagtcgctt catcgctcat atttgataca cttgacattt
     9361 cttttgcaaa tttagatagt tctattttgt ctgttttatc aactttttga acttgttgta
     9421 ccttttttac atccgattta ttctttagat aattatttac tacaatattt gaacttacac
     9481 ttttaatatt cataacctca cctccaaaat tacatcctta tattttaatt atcgctcaaa
     9541 taagaaaaat atttatacga ttcaaataga ttttaaattt tttataaaaa taccccttga
     9601 ttaaaccaat atagaataat ctaaggggat attttttaaa gtattaattt atgtctgaat
     9661 cttctatttt agataatctt acagataatg tttcatttgt aataatactt tctccataag
     9721 caaacaacct gtcatttgca tatatatcta atttttcatc tacattctta tttagtttta
     9781 ttatagaacc ttcttttaaa tctaatatat tttttattgt ttctgttatg tttcctatag
     9841 aaacagccac atttactttt gcattaaaaa cattttgatt ttcctctata atcccagtct
     9901 tttcttctat tagttgacca aaattcaatt catatatatc ttcttttttg ctattcataa
     9961 aacttttctc cttatgtaat cttatctata catatctatc aataagcctt gtatcatatc
    10021 aacagtagaa acaatatcta tattttcttt ttcttcacta agtagtttat tagtcatatc
    10081 ctcaagcttg ttatcaataa tctttacatt cttataatat tgatgctccc agaaactact
    10141 acttttatca agtgaataca tattatctac aacagatttt aggtattctt ttactgcatt
    10201 tttataccta ctaatgtcac cataattttg tgtaagtaca agcctctccc cagccttttt
    10261 tacaccatct aaattccttt ttatatcttc tagtgaagaa gattgtttta caaagtttaa
    10321 gctgtcagaa aacttattat tttcctttgt ctttttaact tctatacttc tacttggact
    10381 tatatttagc ttgtctatat ttatacccat agtaaatcct accctctata atatttcata
    10441 accttaggta tataattttg tgtctccttt ggcattttat acaagtcaga aattgattct
    10501 acgcctctac ttctcattct accaggtcca ccattatatg ccatcaatcc catttctatg
    10561 ctacctccaa acatatctat atattcctta atatgtctag taccagcatc tatattttgt
    10621 tctatgttaa aagggtttgt tactcccaaa tctctgcaat tttctggcat aagttgcata
    10681 agccccttgg caccagcaga agatacagta tttgggttaa aatctgattc aaccttaatt
    10741 attgccttta taagatttgc atctacatta tatttctttg atgcttgctc tacagcattt
    10801 tcaattctcg actttacact tttatctttt gtatcaactt caataaactt accaactgta
    10861 ttatttgcta cactatcaag ttcattaaac agtttttcag aattagatga taaattatat
    10921 ccattagagg tttgttgatt attttgtgat aatgctttca acaatgtcat cataatcata
    10981 tcaaatccag acgaattatt atatccacag ttgcattgat tcgtggtatt actaccagat
    11041 aattgggtca atgccagtat atttattagt gaacttattt cgttcaaaat aaaactcccc
    11101 tttctaaatt ttaaagttat ccttatataa ttcaagatac ttgagttcct ataaaatctt
    11161 cttgttatag actactttgt taaattaatt tcttttatat ttattaaaac tttagattta
    11221 gtttcactac attatgatta tcggataaat aaaaaaaata tttatactta aattatttac
    11281 taattatttt ttaattgact actagacctt ttttattaat actttccttt attatgatta
    11341 ctattaataa ataatcaa
//
LOCUS       DAEMAE010000120        11274 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEMAE010000120 DAEMAE010000000
VERSION     DAEMAE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.122"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(75..5156)
                     /locus_tag="KPH93_002426"
                     /pseudo
     CDS             complement(75..5156)
                     /locus_tag="KPH93_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(5167..6699)
                     /gene="purH"
                     /locus_tag="KPH93_002427"
     CDS             complement(5167..6699)
                     /gene="purH"
                     /locus_tag="KPH93_002427"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG1689234.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(6692..7285)
                     /locus_tag="KPH93_002428"
     CDS             complement(6692..7285)
                     /locus_tag="KPH93_002428"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG1689235.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKGNKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(7279..8355)
                     /locus_tag="KPH93_002429"
     CDS             complement(7279..8355)
                     /locus_tag="KPH93_002429"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG1689236.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(8349..9716)
                     /gene="purF"
                     /locus_tag="KPH93_002430"
     CDS             complement(8349..9716)
                     /gene="purF"
                     /locus_tag="KPH93_002430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG1689237.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(9728..10426)
                     /locus_tag="KPH93_002431"
     CDS             complement(9728..10426)
                     /locus_tag="KPH93_002431"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG1689238.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(10432..10908)
                     /gene="purE"
                     /locus_tag="KPH93_002432"
     CDS             complement(10432..10908)
                     /gene="purE"
                     /locus_tag="KPH93_002432"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG1689239.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
BASE COUNT         3232 a         2253 c         1196 g         4593 t
ORIGIN      
        1 catcacatta ttacatctct tcaaatctaa atatctaaaa tatatattct agctatcaat
       61 taaacttcaa agttctatct aaaatattta actccagatt caaatatctt ttggtctttt
      121 tcacctataa tattcttaaa tacttcctca ccaattctct cagaatgtcc cattttacca
      181 aacactctac catcagcact agttatacct tcaacagcat agaatgaacc atttggatta
      241 aattcaatat cataagtagc ttcattatta aagtcaacat actgagttgc tacttgtcca
      301 ttagcaatta attctctcat aacatcttca ctagcaacaa atttaccttc tccatgagaa
      361 attgctatat tgtgaatatc tccaacatta gtttgagcta accaaggaga tttattagat
      421 gaaattctag ttctagcaat cttagcttga tggcgaccta tattattgta agtaagtgtt
      481 ggagcacttt cacttggaac tcttatttct ccatatggaa ctaatcctaa tttaatcaat
      541 acttggaatc cattacagat acctaacatt agtccatctt tttgagttaa aaattcattt
      601 atagcttctt gcactctagg atttctaaat acagtagcta taaatttacc agaaccatct
      661 ggctcatcac cagcactaaa tccacctggt aacattatta tttgagaaga ttttatttcg
      721 tttactattg tatctataga atcttctatg tctttataag ttaaattctt gaatactttt
      781 atgtttgcat tagctcctgc tctttcaaat gctcttgcag agtcatattc acagtttgtt
      841 ccagggaatg ctggtataaa tactcttggt gtagctatgc ttaatgatga ttttttagct
      901 tctccttgtg atatataact tatagtttct atctttgatt ttacttcttc tgttttagtt
      961 gggaatatag gctctaaagt actacaatgt gctttatata attcatctaa tgcaagttct
     1021 tcacctttga taaatatact agcttcttta actgtacttc caagcacaac ataattgtat
     1081 ccttctaaca tacttaaatc atcatttgct aattcaagta ctatatttcc ataactagcc
     1141 tcaaataatt tctcatctat aaatgctttt tcagcttctt gactaaactt aaatcctatt
     1201 ttattaccaa aagccatttt acttattgct tcacctacac ctgcaaatcc taatgcatat
     1261 gttgataaaa ctttatttcc atgtataagt tctcttactt tgtctaagtt tctctttaat
     1321 tcctcaaaat ctacaacatc attttctgcc ttgcttacac ataacattac tacttttgag
     1381 tctgcttttt taaattctgg agatactact ttcttcgcat ctactgtatc aacagcaaat
     1441 gaaactaatg ttggtggaac ttcaatatct ttaaatgtac cagacatact atcttttcca
     1501 cctatagcag gtatctcaaa tttactttgt gcataaaatg ctcctaaaag tgctgagaat
     1561 ggtttacccc atttttctgg attattgcca agtttttcaa aatactcttg gaatgtaagt
     1621 cttgttgtac tataatttcc tccaatagca accaatttac acacagattc tactactgca
     1681 tataaagcac catggaatgg actccatttt cctacctttg gattgtatcc ataagtcatt
     1741 attgtagaag tattagtttc tcctccaagt actggtatct tagcaaccat accttgtgtt
     1801 ggagtcgctt gatactttcc tccaaatggc ataagtactg tattaccacc aattgtatta
     1861 tcaaacatct ctactaatcc tttttgagaa caaacattaa ggtcagataa tacttttgta
     1921 aacttgtctt tcatacttgc acacttaaca tcttttacta tttcattaga actaaaaaat
     1981 gtattttctt catctacttt agtaacatgg acatcagtag tttgttttac tccgtttgta
     2041 tctaagaact ctctatttat atctacaatt gcttttccat tccagaacat tcttaaatat
     2101 cctgtatctg ttactgtagc tacatgtgta gcttctaagt tttcttctac tgctaattgt
     2161 ataaatttat ctttattttc tttttttata gctactgcca tacgttcttg tgactctgaa
     2221 attgcaagct cagttccatc aagtccatca tattttttag gcactaagtc taagtttata
     2281 tctaagctat cagcaatctc tcctatagca acacaaactc cacctgctcc aaaatcatta
     2341 catcttttta tcatttgagc aacttcttta tttctaaaga atctttgtat ttttctttcg
     2401 ttaggagcat ctcctttttg aacttctgca ctacaagtta agattgactc ttcactatgt
     2461 tctttagatg aaccagttgc tcctccacat ccatctcttc ctgtttttcc tccaagaaga
     2521 actattacat ctcctgcttc aggtctttct cttactacat tctcttttgg tgctgctgct
     2581 ataactgcac ctatttccat tctctttgca acaaagtttt catcatatac ttctgcaact
     2641 tgtccagttg taagacctat ttggttacca tatgaactat atccatgagc agcttctgta
     2701 gttatttttc tttgcataag ttttccagat aaagtatctt ctaaagtcgt tcttgggtca
     2761 gcacttcctg taacacgcat tgcttgataa acataacttc ttcctgataa tgggtctctt
     2821 atagctccac ctaaacaagt agctgctcca ccaaatggtt ctatttcagt tggatggttg
     2881 tgagtttcat ttttaaacat tactagatat ttttccatct taccatcaac ttcaacatct
     2941 acatttatac tacatgcatt tatttcttca ctttcatcta aatcatttaa ttttccattt
     3001 ttctttaact cttttactgc aacagtagct atatccatta aacatatatc tttatgtctg
     3061 ttcacatata cattgtctct tgagttaaga tacatttgat acgcttcttt tactatatca
     3121 ttaaacttac catcttctat ctttacattt tctatttcag tcataaaagt agtatgtcta
     3181 caatgatcag accaataagt atctaatact tttatttctg ttatagttgg atttctcttt
     3241 tctgtatctt taaagtattt ttgaacgtgt tgcaagtctc ctaaagtcat tgctaatcct
     3301 ttttcactta caaatgttct tagtccatct tcatctaaat ctataaatcc atctaatact
     3361 tcaactgttg ttggtatttc agtttccatt tttaatgttt ctggtttttc taaagatgct
     3421 tctctactat ctactggatt tatacagtaa tcttttattt tagaaaactc ttcatcagtt
     3481 attttgcctg ataatatata aacttttgct gtattaatag caggtcttat cccttgattt
     3541 acaatttgta cacattgagc tgcccaatca cctctttggt cgtattgacc aggaagaaat
     3601 tctattgcaa atactctttc atcatttaat tttggtattt cttcataata tactacatct
     3661 aaatttggtt cagagaatat tgtctttgct gcattttcat aaacttcttc tgatattcct
     3721 tctacatcat atctatttaa tattcttaaa ttttctatat tatctatgtg taaactttcc
     3781 accaagtctt tttttaaagc tttagcctca aggtcaaaac cttctctttt ttcaactaat
     3841 actcttctaa ccattgagtc tttattttca gtattcaaca taccttatcc tccattttag
     3901 aaaaattatc ttcctatgtc tgttctatag tgcattcctt caaagtttat cttctttatg
     3961 ttttcataaa ctttttctgc tgcatcctta actgtaactg actttgcagt tataccaatt
     4021 actcttcctc cattggtaac taaattacca tcaagcattt tagtgccact atggaatact
     4081 actatatcat catccaagtt ttcaagacca gttataattt tccctttttc ataactgtct
     4141 ggataccctc ctgatgtgag cataacacat acagcttctt catcactata atttatttct
     4201 atttctttta acttattatc tagtatagct tccattatct catgtaaatc tgtttctaat
     4261 ctaaataaaa cagattgtgt ctcagggtct ccaaatctta cattatattc aagtactttt
     4321 tctccatctt cagttatcat aagaccaaca aataaaatac ctttaaaatt aagtccatct
     4381 tttttgaagc cttctaaagt tttttctaat actgtctctt ttactttatt tgctaattct
     4441 tctgtatata tttcactagg tgaaaaagtt cccattcccc cagtgtttgg accttgttca
     4501 tagttgttta ctttcttatg gtcttttgca cttgccatag gaactattgt atcattatct
     4561 acaaatgcta gaattgaagt ttctattcct tttaaaaact cttcaattac tattttatct
     4621 ccagcagctc caaacttctt atctgacatc atttctttta aagttgctat agcatcttct
     4681 ctattttcag gtattacaac accttttcca gcagctaatc cgtccgcttt tattactact
     4741 ggatatccaa aactatctat ttctgatata gcttcttcta agtttgtata ttctttatat
     4801 tttgcagtag gtatgttatg ttttatcata aattcttttg aaaaagcttt acttccttca
     4861 agttgagcac attttttatt aggtccaaat actcttaatc cttctttttc aaactcatct
     4921 actattccca taactagtgg tacttctggt cctactattg tcagacctat ttcattttct
     4981 ttggcaaatt ttaaaagttc ttctatatta gtatcaccta tatttacaca ttctgctaca
     5041 tttgcaattc ctgcattccc tggtgcacaa taaatttttt ctacattttt ttctttgctt
     5101 agtttccagc atatagcatg ctctcttccg ccaccgccta caaccaatat cttcataata
     5161 tcctccttag tgtttgaagt gtcttattcc tgtaaatacc ataaccatat catacttgtc
     5221 acaagcttct attgaatctt tatcttttat agagccacct ggttgaacta ctgctgatat
     5281 tccatattca tgagctaatg ttacacaatc atcaaatggg aaaaatgcat ctgacgctaa
     5341 aacagctcct gtaaaatctt tatctttgtt attttcaaga gcatttttta atgcccaaat
     5401 tcttgatgtt tgtccacaac ctaaagctag agtttgtcca ttttttacta ttgcaattgc
     5461 atttgatttc atatttttta ctactctcat accaaattcc atgtccttaa gttgtgcatc
     5521 tgttggtttt gcctttgtta cattttcact tttttctgct aatatgttat ttctatcttg
     5581 tatcaataat tttccatcta aatatttcat ttcatatggt tgtaaactat tttctatttt
     5641 agctaatttt aatattctta gatttttctt ttgtttaagt atctctaaag cttctggttc
     5701 aaaatcatat gctactacta tttctaagaa tatttcattt aatttctctg ctgtagcttt
     5761 atctatagta gaagttattc ctactattcc tccaaatatt gaaaccttat ctgcttcata
     5821 acattttgta tatgcttcaa aagcatcttt tcctagacct accccacatg aattagcatg
     5881 ttttatagct actgaaacta cttcttcact atctttaaat tctctcatta cttctaaaca
     5941 tccatgtaaa tcatttaagt tgttaaaaga aagttcttta ccgcctaatt gctcatagtt
     6001 taagattgga ttttttgcat ttgactgtga atataagaaa ccattttgat gtggattttc
     6061 tccatatctt aaagtttgtt ctttttggaa agttaaattt aatatgtcag gatacgtatc
     6121 tccaacttct ccagcaaaat aactagatat taacgcatca tatcttcctg tagttgagaa
     6181 agctttatat gataattttt ttctgtcttc taatgtcatt gtatctgttt ttaatttttc
     6241 tattatagaa tcataatctt ttatatctac tactactatt acatctttat agtttttggc
     6301 agcagaacgt atcatagatg gtccaccaat atctatattt tcaattattt catcatgact
     6361 ttttccagct tttaaagttc cttcaaaatc atatagattt acagctacta aatctattga
     6421 atgtatttta tgctcattta cagtttctac atgagattct ttatctcttt tataaagtat
     6481 accaccatgt atatatggat ttaatgtttt tactcttcca tctaatattt ctggaaaatt
     6541 tgtaacatct tcaacttgca ttacattaac accattttct tttaatgttt taaatgtatt
     6601 tcctgtagat ataatttcat aatctaactt atttaactct ttagcaaatt ccactacacc
     6661 agtcttgtca gttacactta ttaaagctct cttactcatt tataaacact ctcctacctt
     6721 gaagttttaa tttattttca caaaaaagac ttatactttc ttttaaaatc ctatgttcaa
     6781 cttctaacac tctttttgca agtgtctttg catcatcatc ttgatttacc ttaactacat
     6841 cttgcattat gattggccca gtatcagcac cttcatcaac aaaatgaact gtagcaccag
     6901 ttactttagc tccgtaatct ataacacctt gatgaacttt ttctccatag aatccagctc
     6961 cacaaaatga aggaattaat gatggatgta tgtttatcat tttgtttcta aactcattaa
     7021 ccagctttgg gctgattatt tttaaataac cagccaaaac tactaaatct attttatttc
     7081 ccttaagaat ttctattatc ttgtcttcat cagtttcaca aattgctttt atgttatgat
     7141 ttttagctct ctctagccca taagcacctt gcttacttga gataactact ttaacctgac
     7201 cttttatttc tccagactca gtcccatcta taactgcttg taaatttgtt cctccaccag
     7261 atattaaaac tcctatattt agcataattc tacaccttca tgactatcta ctacttcacc
     7321 aattatatac gctttatctt ctaataattc actatatttt ttatctacat aagcagcatt
     7381 gtcattttct ctattattta taaagtttac aacatcatca gcattttctt tatctactat
     7441 taaaactagc ccaataccca tgttaaaact cttatgaagt tctctctcat caacagcatt
     7501 aaaaccttct atcattttaa atataggagg cttctcccaa gattttttat tgatatctag
     7561 tcctaaccct ttaggtataa ctcttgttat attttctata agccctccac cagttatgtg
     7621 agctatagct tttatgtcat gtttagctaa tacatctaaa gctaatttaa catatatttt
     7681 agttggtgtt aaaagtgctt ctccaactgt cattcctagt tcttcaacat attgttccat
     7741 tttataattg taagtttcta aaaatatctt tcttataaaa gaaaatccat tgctgtgtac
     7801 tccacttgaa gatataccaa ccaatacatc tcctgattta acgttgtttc cagaaacaat
     7861 cttttctttg tctgctattc ctacagaaaa tcctgctaaa tcatagtcat cttctccgta
     7921 cattccaggc atttcagcag tttctccacc aattagtgca caaccagaca ttttacaacc
     7981 atctgcaatt ccaccaacta ttttttcgat atgctcagga actaatttcc caagagctat
     8041 atagtcaagg aagaataatg gttttgctcc ttgacaaatt aaatcattca cacacattgc
     8101 aactaaatct attccaacag tatcatgctt gtccatcatc tgagctattt ttaacttagt
     8161 cccaacacca tctgtagatg caagcaaaac tggttctttc atacctacaa aatcttttaa
     8221 actgtataat ccactgaagt tacctaaatc tccaactaca tttccatcat aagttccttt
     8281 tattttacct tttataaggt ctactgctct atttccttca tctatatcta cacctgattc
     8341 tttatatgtt aacatgaaag tagttcctcc ttatcaattt tcttaactgg gtattctcca
     8401 tcaaaacaag ctttacagaa agtagactta tgttctgttg catctaacat tccttctata
     8461 tctaggaatt ttaaactatc acatccaata tattctctca tttcttctac gttgttactt
     8521 gatgcaatta atttagagcg atttggagta tctattccat agtaacaaga gtaagctact
     8581 ggaggagaag ttattctaag atgaatttct tttgctcctg cttctcttaa agattttact
     8641 aattgtttag atgtagttcc tcttacaatt gaatcgtcta ctaaaattat agactttcct
     8701 tttattattg ttgaaagagg atttaatttt atttttactg ctatttctct ttcttcttgt
     8761 gtaggtttta taaatgttct tcctacatat ctatttttaa ctaagccttc acttattttt
     8821 aatccactag catttgcata accaatagct cctggccaac ctgaatctgg aactggaact
     8881 accatatctg cctttacagt ttcattctga gctaatctct caccacattt aattctaaat
     8941 tcatatgcat ttacattgtc tatagttgca tcatttcttg caaagtatat gtgttcaaat
     9001 atacaacttt tcttaactgg tttataattt tctgaataaa aatatgattt tagttctcca
     9061 tctttaacta ctattatctc tcctggctct acatctctta taacttctcc gccaagtata
     9121 tctattgcac aattttctga tgcaaatata tattcatcac cctttttacc aagtacaaga
     9181 ggtctgaatc catgtgggtc tctaactgca accaattctt cttgactcat tattacaaga
     9241 gcatatgcac cttttatata atccattgtc acttttaaac tttctactat atcccctttg
     9301 taatatctcg caagtatata taaaatcact tctgtatctg aattagtttg gaacatatat
     9361 ccatcttctt ctagcatatc ttttaaataa tttgcattaa ctagatttcc attatgagct
     9421 atggctaatt gtctttttct acaacttcca actaaaggtt gacagttaga aacatgactt
     9481 cctccagctg ttgaatatct aacatgtcca atccctatat ttccttttaa cttagataat
     9541 tcattttcct taaaaacatc tccaactaat cccatatctt ttttgtactt aatttctcca
     9601 tcatctaaaa gagctaaacc acagctttct tgccccctgt gttgcataga atataaagaa
     9661 taatatagtt ctttagttac atctttatta gagtaaatcc ctaaaactcc acacatacat
     9721 ttgcctccta tttattattc attcttgaaa gtacttccgt ataaccttct acaaggtctc
     9781 caaggtcttt tctaaatctg tccttatcta acttttcatt tgtatttaca tcccaaagtc
     9841 tacaagtgtc tggtgatact tcatctgcca gtattatatt tccttcagaa tccttaccaa
     9901 attctatttt aaagtcaact aactttaggt ttaattttaa gaaaaatgct tttagtaatt
     9961 cgtttatttt taaagtttct tctcttaaga attttaattc ttctctagtt gctaacttca
    10021 ttgccactgc ataatcatca tttagcattg ggtcaccata agcatcattt ttgtagctta
    10081 tttcaaaaat tggctcatca aaaactacac cttcttcaag accaactctt ttacagatag
    10141 aaccagcagc aatatttctt actattactt ctaatggtaa aatctctact tttttaacta
    10201 acatttctct atctgataaa ctttttataa agtgagtatt tatattattt tcttttaaca
    10261 tttcaaagat tattgttgat atcttattat ttagtatacc ttttgaagat atttctgctt
    10321 ttttttctcc attaaatgct gttgcatcat ctttataata aacaacatat tcattttcat
    10381 tatctgtaga atatacttgt tttgcttttc cttcatataa taacatttct gctaccccct
    10441 taaattcttg tcgtcttcta acaccttttg tgccatctct tctctatatg atttcaaatc
    10501 ctcttttaat tttgggtatt ttaaagtcat tatttgtaat gccaataaag ctgcgtttaa
    10561 acctaagtct attgtaactg ttgcaactgg gattcctttt ggcatttgaa ctattgataa
    10621 aagtgagtct agtccatcca tagttgatga tttaataggt aatcctatta caggaattaa
    10681 tgtttgtgaa gctattacac ctggtaagtg tgctgctttt cctgctgctg ctattattac
    10741 atcagtatca tcttctattt cttttaggaa tattgaaagt tgttctggtg ttctatgagc
    10801 tgataatgct cttgctacaa cttctattcc atatttttca agcagtttta tcccttcttc
    10861 taattttgga taatctgatt ttgaacccat aactactgct actttcatta gacttcccct
    10921 ctccgtttat aagttatttt acactctaaa tcattcgtat tattatacga attatacacc
    10981 ttaaatttta tattaatata catatattat caactctttt ttgaaatttt ttaaaaaaaa
    11041 tatttcttgt atttttttca ttcgctacgt atcgaacttt atttatgttt ttttgttaaa
    11101 ataaaaaaca gactatctca aaattgattt tctaattaaa ttttaagctc aatacccttt
    11161 ttaaaatctt gtatattatt ttttattact tcttttttaa ttaatacctt ggatttataa
    11221 tcttgttttt tagttctgaa tgttagcttt acttagataa aaccaataca aagt
//
LOCUS       DAEMAE010000121        11108 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEMAE010000121 DAEMAE010000000
VERSION     DAEMAE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.123"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..1150
                     /locus_tag="KPH93_002433"
     CDS             83..1150
                     /locus_tag="KPH93_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG1689240.1"
                     /translation="MKNKLIYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     QREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGKKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPASITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1320..3221
                     /gene="cooS"
                     /locus_tag="KPH93_002434"
     CDS             1320..3221
                     /gene="cooS"
                     /locus_tag="KPH93_002434"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG1689241.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKIADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3214..3687
                     /locus_tag="KPH93_002435"
     CDS             3214..3687
                     /locus_tag="KPH93_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1689242.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3662..4888
                     /locus_tag="KPH93_002436"
     CDS             3662..4888
                     /locus_tag="KPH93_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1689243.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENIYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVK
                     GVAFSNGEILDADMVIVATGVKPNADFLDETGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFIFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(5027..6214)
                     /locus_tag="KPH93_002437"
     CDS             complement(5027..6214)
                     /locus_tag="KPH93_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1689244.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     KNLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6487..7311
                     /locus_tag="KPH93_002438"
     CDS             6487..7311
                     /locus_tag="KPH93_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1689245.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVRETIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCNFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7440..8705
                     /gene="gluD"
                     /locus_tag="KPH93_002439"
     CDS             7440..8705
                     /gene="gluD"
                     /locus_tag="KPH93_002439"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG1689246.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8898..9599
                     /locus_tag="KPH93_002440"
     CDS             8898..9599
                     /locus_tag="KPH93_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1689247.1"
                     /translation="MDFFPPHDYKLEELDWIFRHELMHFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVAGL
                     NKQSDIRKRVINMFSEKHKNGILMALIISLFSSITFFKFDSTSIIEENDFLERSRYTF
                     EGRQEDMFVTIDFTYADAPIKLRKEHEENCRANEIVPKDSGSIEIIPRFYKDFMDLKN
                     KLDLILMLILLIFYI"
     gene            9884..11017
                     /locus_tag="KPH93_002441"
     CDS             9884..11017
                     /locus_tag="KPH93_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG1689248.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTIGNSILLYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDEEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELYWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGVEYYDSLLND"
BASE COUNT         4351 a         1179 c         1954 g         3624 t
ORIGIN      
        1 atgtattgat tatatttaaa aacgttatac tctaaaaaag tatattttat ttaaaaattt
       61 attaatacaa aggagattta ctatgaaaaa taaattaatt tacatatgta ttatgagtct
      121 attgctattt gtagtaggat gttctaataa tccaaataag gaaagtacca ttaaaaaaga
      181 ctctaagaat aaccaaagcc aagttaaaga agaacaaaga gaacctgtga aaaaagatat
      241 aactataagt ttcgctggtg atgtaactat ggggaactat aaaggttcat catattatgg
      301 aagttttgac catgaattta aaagacaagg agaagattac aattattttc ttaaaaatgt
      361 aaaacctgta tttgaaaaag atgacttaac agtggttaat ttagaaggtc cacttactac
      421 agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga aagcctggat atgtaaatat
      481 tttaaaatct ggaagtgtag aggcagtaag tattgcaaat aatcattcag aagattattt
      541 tgaagaagga aagaaagata cacagtttat attggacgag aataaaataa attattttgg
      601 attagggaaa gatgctgttg tagatgtcaa aggaattaaa gtgggtctat tagggtataa
      661 tggattatca actgaatata atgaaaaaaa cttaaaacaa atggaagatg atattaagtc
      721 cttaaaaaag aagtctgatg ttgttgtagt gtactttcat tggggaatag aattagatta
      781 ttatccagat aaaaaacaaa aagattttgc tcattatgct atagacaagg gagcagattt
      841 agttatgggg tctcacccac atgtgattca aggaatagaa aaatataaaa ataagtatat
      901 agcttatagt ctagggaatt tttgctttgg aggaaataag aatccttctg atacagatag
      961 ttatatatat caacaaacct ttactttttc agataataaa ttaacttcaa ttaaagagcc
     1021 aaatataata ccagcttcaa taacatctag tagctccaga aataattatc aaccaaaagt
     1081 attggatgga agcgagaagg atagagttct aaataaactt gaaaaaataa gcaaagattt
     1141 aaataaataa aatgtaaaaa aaaataaata tatatattta ttttaataat ttatattttg
     1201 tttagggtat gggaaatcgt ggtattaaat aagtagattt taatagttaa attatagtca
     1261 attataataa agctatttaa tctacttttt taatataaaa atataagggg gaatttgtaa
     1321 tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa agttttgtag
     1381 cttcaaagga tgttgaaaca gcatttcata gaacagaaga tcagaaagtt aagtgtggtt
     1441 ttggacttca gggggtttgt tgtagactct gttcaaatgg accgtgtaga gtaactccta
     1501 aatcaccaag aggtatatgt ggagcagatg cagacactat agtagcaaga aactttttaa
     1561 gagcagtagc atcaggagca gcttgttatt tacatgtggt agaaaataca gctaagaatt
     1621 taaaaaatgt tggaataact aaaggtgttg ttaaaggaga aaaaacatta aatcaattag
     1681 cagagatgtt tggaatagaa agtgatgaaa agtatgataa atgtataaag attgcagaca
     1741 aagttatcaa cgatttgtat aaatcaagag atgataaaat ggaattagta gaaaaaatag
     1801 cttatgcacc tagagtgaaa aagtggaaag agttaggtat aatgcctggt ggtgcaaagt
     1861 cagaagtttt tgacgcaata gtaaaatcat caacaaattt aaacagtgac ccagtagaca
     1921 tgctagttaa ttgtttgaac ttaggtatat caacaggatt atatggtctt acactaacta
     1981 atcttttaaa tgatgttatg ttgggagaac cagttataag aatggctcct gtaggattta
     2041 atgtaataga cccagactac ataaatataa tgataacagg tcatcaacat tcaacatttg
     2101 caaatttcca agatagatta aaagatgaag atgttataaa attagctcaa tctgttggtg
     2161 caaaaggatt taagctagta ggatgtacat gtgttggtca agatttacaa ttaagaggag
     2221 aacattatca agaagtattt gctggacatg caggtaacaa ttttacaagt gaggctgttt
     2281 tatcaactgg agcaatagat atagttcttt cagaatttaa ctgtactata ccagggcttg
     2341 aaccaatagc agataaatat aaagtaaaaa tggtttgttt agatgatgtt gctaaaaaag
     2401 cgaatgcaga atacataggt ttagacagaa gtaaattaga tgaacttagt aacactttaa
     2461 tagaaaaagc attggagtct tataaggaga gaagaggaag tattgaaata gatataccaa
     2521 aagaccatgg atttgaacaa tctttaactg gtgtaagtga aaagaacctg aaagcgtttt
     2581 taggagattc atggaaacct cttattaatt taattgcaga aggaaaaatt aaaggtgttg
     2641 cagctgttgt aggatgttca aatatgacag caggaggaca tgatgtaaat actgttgaac
     2701 ttactaaaga attgattaaa aaagatataa tagtactatc tgcaggatgt tctactggag
     2761 gattagaaaa tgttggtctt atgtcacctg gagcagaaga attggcagga gaaaacttaa
     2821 aagaagtatg taagacttta ggaataccac cagtattaaa ctttggacca tgtctagcta
     2881 taggaagact agaaatagta gcaacagaat tagcagctga acttggaatt gacctaccac
     2941 aattaccatt agtgctttca gctccacaat ggttggaaga acaagcatta gctgatggag
     3001 cttttggtct tgcccttggc ttacctcttc atttagcttt acctccattt ataacaggag
     3061 gaaaattggt aacagaagta ttaacagaaa aattaaaaga cttaacagga ggacatgtta
     3121 tagtgaatcc agaccctaaa tcttcagcta atcaattaga agaaataata atagatagaa
     3181 gaaaaaattt agggttaaag gatgtggaat gtaatgcata gaatttttat aaataaagat
     3241 ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa taaagattat
     3301 gacatgtaca ctcttgattt agaaaatata gataatgata gtagagggca tatagaatta
     3361 gatagtaaac ataataatcc agttccaatt ctttgtagac actgtgatga acctgaatgt
     3421 gtattagcat gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat cgtttcatat
     3481 gatgaagaga aatgtggttc atgttatatg tgtgtaatgt cttgtccata tggattatta
     3541 aagccagatg atagaagcaa gcaaaacata cttaaatgtg atttatgtaa agatgaggag
     3601 tatcctagat gtgtagctaa ttgtccaagt ggagcaattg aattacagaa ggaggaaaat
     3661 gatgaattat gtagtgttag gggctagtgc agctggtata aatgcagtaa aaacattaag
     3721 agaattagat aaagattcaa atatagtagt aatttctaaa gatgaaaaca tatactcaag
     3781 atgtatgctg catcatgtta tatctgaaca cagaacttta aaacaaataa attttgttga
     3841 tgaagatttt atggagcaaa ataatgtaaa atggattgca ggtaagactg ttaaaggtat
     3901 agacataaat aaaaaagtag ttcagacaga agacattact gtaaattatg ataaactttt
     3961 aatagctacc ggtgcatctt ctgctattcc tcctataaaa aatttaagag atggaaactt
     4021 tgtatattct gtgagaaaca tagatgatat ttataaaata aaagaaaaag cacaaaattc
     4081 taaaaatgta gttataatag gtgctggttt agttggtata gatgcactag tgggattatt
     4141 taaatatgag cagttaaata tttctgtagc ttttatggaa aagtacattt tagatagaca
     4201 attggatgaa tatacagcat cagtgtatga aaataagttc aaagaaaaag gtgtaaaatt
     4261 ttacccaagt gcatcaatac aagaaattgt attggataac agtaaaaatg ttaaaggtgt
     4321 tgcattttca aatggtgaaa ttttagatgc agacatggta attgtagcta caggggttaa
     4381 accaaatgct gactttttag atgaaacagg aatagaatat gatagaggca taataataga
     4441 tgatatgtgt caaacaacac aaaaagacat atatgcagca ggagatgttg taggtaaaaa
     4501 tgctatttgg cctctggcag taaagcaagg aattgtagct gcatataata tggttggaaa
     4561 agataagaag atagaagatg aatttgcttt taaaaatagt atgaatttca tggatatacc
     4621 aactatttct ataggaatga atacacctgt agatgatagc tataaagtat taaccagaca
     4681 tggaattaat gattataaaa agtttatttt taaagataat gtaatctatg gagcagttat
     4741 acaaggagat atttcatatg taggtgtatt aacttattta ataaaaaata aagttgaaat
     4801 atatgattta gaaaatagga tatttgatgt atgttatgca gattttttta atataaaaga
     4861 aaatggagaa ttttgttata gcgtatagta tatacatgga gtaaggttta taaatacagt
     4921 aaatcaataa atagagttat tctaaaataa gaaaaatgac aaatcttatt tagaaatagc
     4981 tctatttttt gtaattaagt ttaaattata ttcaatttgt aaaagattat tttgaacaat
     5041 tatacatata ctctctagtt agaggtaaat cattttttat gcctttagtg aaaagtattt
     5101 gatgtatatc tataatacca gtgttgaaag cagcagcaca agaacaaagg tataagtccc
     5161 acattcttat aaacttatca tcaaacattt ctttaatctt tggaagattc ttagagaagt
     5221 ttttttgcca gtatagtaag gtttttacat aatgtaatct tagactttct acgtctatag
     5281 tatgatactt gtaatcacta caaatatgta tcatttctct taggctagga ataactcctc
     5341 ctgggaaaat atattttttt atccaagggt ctccataaga ttcctgcaat ccacttatat
     5401 agtgaagtaa aaataatcca ccatctttta aaacagcatt tacgttttta atgaaaagtt
     5461 catagttttc tctaccaaca tgttcaagca tcccaacgct tacaactcta tcaaacataa
     5521 ggtctgattt ttttaattct ctatagtcca taagtcttac ctcaaggtag tcttgaaggt
     5581 tctcttcttt aattcttgcg ttaaattcct taaattgctc ttcgctaagc gtaattccaa
     5641 cgccgtgaat tttatatttt tttgcagctt ctattagtaa aaatccccaa ccacaaccaa
     5701 tatcgagaag gctcatacct tcctctaaat ttagtttagt taatatgtgg tgaactttat
     5761 tgacttgagc atcataaaga gtatcattat catttctaaa gtatgcacaa gaataactaa
     5821 gtgtcttatc taaccataag ctataaaaat cgttaccaat gtcatagtgg gcatgtattt
     5881 ctttcttttg atgtctcatt gaatttgatg agtgcaatag atgttttaca gctgaattat
     5941 caatattgaa tttgtctatt tgttttaaaa gtagatttaa agttaagaat aaatctccct
     6001 caacttcaat gtctctattt atgtaggctt ctccaagtgc taaagatgta ctttttaata
     6061 gttcagactt gctaatatca ttatttacta taactttgaa ctgaggggag ccctctccaa
     6121 ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg tgtttcacca aatctctcta
     6181 aatagtcttt taaaaaattt tttgtaatat tcatattacc agctccttct aatgtaaatc
     6241 cagtaagttt ccctgcttaa aatatattat agtccttttt ttaaaaaagt aaaatttttt
     6301 caataaattt tttatttaga ggaaatttat tttttataaa gaaatttcat gttgtagtat
     6361 attttttgcc tataaatttt ggtactggat ataatattaa actgttaatg atataataaa
     6421 tttaatctat aaagtaaata aaattttgta ataaatagtt aaagctattt tataaggaga
     6481 tataggatgg aagtatatgc tttggttggg tcaagtggca cagggaagag ctttaaggct
     6541 ttagagttcg catatgaaaa tgatatagaa tatataatcg atgatgggat tttaatatac
     6601 aaaaacaagg ttttagctgg gatatcggca aaacaagcaa atactactat agaagcagtt
     6661 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaaac aattaaaaaa
     6721 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta
     6781 gagagattgt caattggaag ggtctgtaac tttataaata tacaagatat aagtactaaa
     6841 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca
     6901 gcagttgaga taaaaagtat ggcaagtggt cttagtataa attctttgaa gagacttttt
     6961 agaaaaggaa ataataggaa catggtagtt ttagaaaaaa caattataag accaactttt
     7021 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa
     7081 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat
     7141 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa
     7201 gatatacagc agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa
     7261 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac
     7321 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaatttagga
     7381 aatggttttt tctaaatgga gtatacataa ttataacgta tttataaggg ggctttcaaa
     7441 tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc tcaagtaaaa aatgcatgtg
     7501 ataaattagg tatggaacca gcagtttatg aattattaaa agaacctatg agagttatag
     7561 aggtttcaat tccagtaaaa atggatgatg gttctataaa aacttttaaa ggatttagat
     7621 cacaacataa tgatgcagta gggccaacaa aaggtggaat aagattccat caaaatgttt
     7681 caagagacga agtaaaagct ttatctatat ggatgacttt caaatgttct gtaacaggta
     7741 taccatatgg tggaggtaaa ggtggaataa tagtagatcc atcaacttta tctcaaggtg
     7801 aattagaaag attaagtaga ggatacatag atggaatata taaattaata ggtgagaaag
     7861 ttgacgttcc tgcaccagac gtaaatacta atggtcaaat aatgtcttgg atggttgatg
     7921 agtacaataa attaactgga caaagttcta taggtgttat aactggtaag ccagttgaat
     7981 ttggtggttc tttaggaaga acagctgcta ctggatttgg tgttgctgtt actgcaagag
     8041 aagctgctgc taaattagga atagacatga aaaaagctaa aatagctgtt caaggtatag
     8101 gaaacgttgg ttcttataca gttcttaact gtgaaaaact tggtggtact gttgtagcta
     8161 tggctgaatg gtgtaaatca gaaggttctt atgctatata caatgaaaat ggtttagatg
     8221 gtcaagctat gttagattat atgaaagaac atggtaactt attaaacttc ccaggagcta
     8281 agagaatatc tttagaagag ttctgggctt cagatgttga tatagtaata ccagctgcat
     8341 tagaaaactc tataactaaa gaagttgctg aatctataaa agctaaatta gtttgtgagg
     8401 ctgctaatgg accaactact ccagaggctg atgaagtatt tgctgaaaga ggaatagttc
     8461 ttactccaga tatattaact aatgctggtg gagttacagt ttcttacttc gagtgggtac
     8521 aaaacttata tggatactac tggtcagaag aagaagtaga gcaaaaagaa gaaatagcta
     8581 tggttaaagc atttgaatct atttggaaaa tcaaagaaga gtacaatgtt acaatgagag
     8641 aagctgctta catgcattca attaagaaag ttgctgaagc tatgaaatta agaggatggt
     8701 actaggattt actcttaata aataatagga tataattatt taagatataa aggggctgta
     8761 catagtgcag cccttaaatt atgtagctta caatgaagtg agctattttt attttaactt
     8821 ataaataact tgataaatta tgtaatttag aatgcataaa taagatgggt gaaaattaat
     8881 ggttgattta attttgaatg gattttttcc caccacatga ttacaaatta gaagagttag
     8941 attggatttt tagacatgaa cttatgcatt ttaaaagcaa agaccttcat ttaaagtatc
     9001 tagtattatt tttaaaaata gtatattggt ttaatccatt tatatatata atggaaaaat
     9061 atatggattt ggattgtgaa ctttattgtg atgagagagt tttaaaaaat tgtactgtac
     9121 aacaaaagca agaatatgct ctaactattg taaattcaat gaaaagaggt ataaattttt
     9181 caaataaatt tgtagctgga ttaaataaac aatctgatat tagaaaaaga gtaataaaca
     9241 tgttcagtga aaaacataaa aatggtatct taatggcttt gattataagc ttgttttctt
     9301 ctattacatt ttttaaattt gattcaacta gtattattga agagaatgat ttcttagaga
     9361 ggtcaagata tacgtttgaa ggaaggcaag aagatatgtt tgtaacaata gattttacat
     9421 atgcagatgc acctataaaa cttagaaaag agcatgaaga aaactgtaga gccaatgaaa
     9481 tagtaccaaa agattcaggt tcaatagaaa ttattcctag attttataaa gattttatgg
     9541 atttaaaaaa taagttagac ttaatattga tgttgatatt attaattttt tatatttaag
     9601 taattgaaaa ttttattatg gattcaattt tatatgtgtt taagtatgaa atttatagat
     9661 agttgaaata attaaatatg attgatgcat aggaatcatt actaagctat aattaattat
     9721 aagggagttt aaataaatta tgagttacac aattataaaa tgataaaaaa tagttatctg
     9781 ttacaaaaaa tgtatctttt taattaataa agataatatg aaaacttaaa aggtaaataa
     9841 acttaaatat aaaatactct taaataagat aggagaaaaa tgtatgtcta actttattct
     9901 aaatgggatt tttagaacag ttatagtaag tagtctaagc attcttctga tattgatttt
     9961 caagaaaaat gtatttaaaa gatttagcaa aaaatttaat tattatatat ggatgatagt
    10021 tgttattaaa ttatttttac catttacata ttatactttc acaataaata tcttgagaag
    10081 taaaaaacac attaatatta ataacataaa tttagaggga tttaataatg tttcaactat
    10141 aggtaatagt attctattgt acacttggat tataactgta gtagtgtatt taatttacac
    10201 tatatttaaa tatataaaac ttaaaaattt aataaatgac ttatcttatg atgttgatga
    10261 tgaagagatt gtaaatcttt ataaaaatat attagaagaa tttaatataa caaaagatat
    10321 aaaactcaaa tactcttatg aagttgaaac accagcattt tttaattcat gtgtgctttt
    10381 gccaccacat gagtataaat tgaaagagtt atattggatt tttagacatg agcttatgca
    10441 ttttaagagt agggaccttt atttaaaata tgtaatcctg tttttaaaaa cagtatattg
    10501 gtttaatcca tttatatata taatggataa acatatagat ttagattgtg aactttattg
    10561 tgatgaaaga gttttaaaaa atcgaaattc agaagaaaaa aaggattatg ctttaacaat
    10621 tctaaatgca atgagaaaag gttcaaatac ttcaaataaa tttatagctg gccttcataa
    10681 gcaatctgat attaaaaaga gagttatgaa tatgtttaat gaaaagtata agaatggtat
    10741 cttaatggca ttaactttat gtttattatc ttctattact tttttgaagg ttaattcaac
    10801 tagtattgtt aatcttaata tattgccaaa aggaatggaa gaaaaagatg ttactaatag
    10861 tgcaattgtt acaatgatag aatttacata tgcagaagct cctaaaagta tgagagaaga
    10921 acataaaata aattgtgaat tcttaggttt aatacctaaa gactctgata aaataggtgt
    10981 tggtgtagaa tattatgaca gtctattgaa tgattaaata aatattattt taacagatat
    11041 taattataaa gttttgaata gtaatttgtt gagaaataga tatatcctta taagaataga
    11101 tacttata
//
LOCUS       DAEMAE010000122        11026 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEMAE010000122 DAEMAE010000000
VERSION     DAEMAE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.124"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..926
                     /locus_tag="KPH93_002442"
     CDS             162..926
                     /locus_tag="KPH93_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG1689249.1"
                     /translation="MQIIDININNLDNEHICCSISEKKGENCVFSKKTWMKNNFNKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYMFINCFWISGKFKGQGYSNLLLQECIDD
                     AKSKGKCGLVVLSSSKKMPFLSDAKYLKYKGFCLADTLEPNYELLYFPFIDNASIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYALILKKIANFRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTSEILSEKKIEKFLDTNIKKI"
     misc_binding    1146..1359
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1613..2647
                     /locus_tag="KPH93_002443"
     CDS             1613..2647
                     /locus_tag="KPH93_002443"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG1689250.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLINHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLISVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2688..3905
                     /gene="argJ"
                     /locus_tag="KPH93_002444"
     CDS             2688..3905
                     /gene="argJ"
                     /locus_tag="KPH93_002444"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG1689251.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3920..4780
                     /gene="argB"
                     /locus_tag="KPH93_002445"
     CDS             3920..4780
                     /gene="argB"
                     /locus_tag="KPH93_002445"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG1689252.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIQGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4810..5997
                     /locus_tag="KPH93_002446"
     CDS             4810..5997
                     /locus_tag="KPH93_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG1689253.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNGNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNIDSTVCAIILEAIQGEGGVNVLEKDYVLELVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVATSVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6107..7129
                     /gene="argF"
                     /locus_tag="KPH93_002447"
     CDS             6107..7129
                     /gene="argF"
                     /locus_tag="KPH93_002447"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG1689254.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTTIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYETRINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7256..7873
                     /locus_tag="KPH93_002448"
     CDS             7256..7873
                     /locus_tag="KPH93_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG1689255.1"
                     /translation="MKKIILALTTFLLAVSLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDKKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMLGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFKCSKKDNKWIISSVNDVVADVLLSNLVTAGQEFSNSMNALE"
     gene            complement(8091..8789)
                     /locus_tag="KPH93_002449"
     CDS             complement(8091..8789)
                     /locus_tag="KPH93_002449"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG1689256.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PLLNIVEETVKKLPTLSQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNTL
                     IQNIKIDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8951..10168)
                     /locus_tag="KPH93_002450"
     CDS             complement(8951..10168)
                     /locus_tag="KPH93_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG1689257.1"
                     /translation="MLGKKCMDYLQVLGKISSTTNGLTRLILTQEHKKSIDLISSWMK
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNKSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10360..10773)
                     /locus_tag="KPH93_002451"
     CDS             complement(10360..10773)
                     /locus_tag="KPH93_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689258.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERACKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         4329 a         1193 c         1831 g         3673 t
ORIGIN      
        1 tatttaatta tatatgcaat ttgataattc aaataatcta aaaataatat tatgtaaaat
       61 aaaaatatag atgacataat gttgttatat tcaaaatgtt aaaataaaaa atatatagaa
      121 tatatttaaa aattattatg taattagaaa aggagataag tatgcagatt attgatataa
      181 atataaataa tcttgacaat gaacatatat gttgttcaat ttctgaaaaa aaaggagaaa
      241 attgtgtttt ttctaaaaaa acttggatga aaaataattt taataaaggt ttagtcttta
      301 aacgatttga tgtaaggggt aaagctttta tagaatatat tccagcagaa aatgcatggt
      361 gtcctattac tgcagacaat tatatgttta tcaactgctt ttggatatct ggaaagttta
      421 agggacaagg atattctaat ctattgttac aagagtgtat tgatgatgct aaatcaaaag
      481 gtaaatgtgg attagttgtg ctttcgtcta gtaaaaaaat gcctttttta tctgatgcta
      541 aatatctaaa atataaaggc ttttgtttag ctgacacatt agaaccaaat tatgagctat
      601 tatattttcc atttattgat aatgcttcta ttccatgttt taagaattgt gtaaaacaaa
      661 gaaagttaaa tgagaatggt atagttttat attatagtaa tcagtgtcca catacagaaa
      721 aatatgctct tattttgaaa aaaattgcaa attttagagg tattgatgta aatcttataa
      781 aattcgaaac agcaaaagaa gcacaaaatg ctcctgcacc atttactaca tacagtcttt
      841 tttttaatgg tgaatttgta accagtgaga tactttctga gaaaaagatt gagaaatttt
      901 tagataccaa cattaaaaaa atttaactaa aaagagatgt aaatattgta ttttgagagt
      961 atattatttt ataatctaat atatgggaat tttttaagtt gatgaatgat aatataaaaa
     1021 ttataactca atttagatac aaaatgctgg aaattaacca tatagaaaat atttccaaaa
     1081 attttaaaat tatattgaca aagttaaaaa aataaactat catataaaac aataagaaaa
     1141 taaattaaaa tcctgtgaaa aagagagtag cataaaactg gttgatagag agctgggaat
     1201 ggtggaagcc tagtaacgaa ttttattgtg aatagagctt tggaggaggt taccagaagt
     1261 aatagtatgg taattggtga atctcgcctt aagagattag agtgggtaag attatcttat
     1321 caattagagt ggtaacgcgg gtgtatattc gtctcttggt ctttgaccag gaggcttttt
     1381 taattttaaa tatacatttt gtagatactt tttaatttga gttttaatga ttattgtaat
     1441 tttaccaata agagtggtac cgcggaaaat cgcctcttag tatttataat gctaagaggt
     1501 tttttgtttt aattagaata ttttaatcta cgtattatca aatgattatc atatagtaaa
     1561 gtggaaaggt agaattttaa gactatttga atataaaaat ggagggaaca aaatgaataa
     1621 aataaaagtt ggtattattg gtgcaactgg atatgtcggg gcagagttga ttagattatt
     1681 aataaatcat gataaagtcg aagtgacagc gataggttca aattcgtatg ttggaaaaga
     1741 tatagttgat atatatccaa gtataggata taaaaataac atgatatgta tagaaaatga
     1801 aaaagttata gatatgtgtg atgttgtgtt tacagccctt ccacatggag ttagtgaaaa
     1861 gtttgtaata aaggcaatta aatcaaaaaa gaaagttata gatttaggtg ctgactttag
     1921 aataaaagat gaagaagtat atagtaaatg gtatggagta tcctttatag ataaaatctt
     1981 gcataaaaaa gctgtatatg gattaagtga aatatacaaa gaagatataa aagatgcaga
     2041 tataattgca aatcctggat gttatccaac tagtatttct ttaccattga tgccattact
     2101 aagttctaaa ttgataaaaa ataataatat aataattgat tctaaatcag gtcttacagg
     2161 tgctggtaga gaattatcga ttagtagtca ctttacggag gttaatgaaa atatcactgc
     2221 atataaaata gggaaacaca gacatactcc agaaatagaa caaaatttaa gtgaatcctg
     2281 caaagaaaaa gtaagtgtag tttttacacc aaacttaatc tcagtaaata ggggaatctt
     2341 atcaactata tattgtacaa aagaagataa tatttctata aatgatatac ataggaaatt
     2401 aactgattat tatgaattta aagaatttat agaagttctt ccattagata aggtagcttc
     2461 tttaaagaat gttaggttct caaataaatg tgtaatatca ttacatgaaa atggagatac
     2521 attgataatc tgttcagcta tagataatat gattaaggga gcagcaggtc aagctataca
     2581 aaacatgaat ataatatttg gtattgaaga aaatacaggt ttaaaaaata ttgcaccatc
     2641 attttagaaa taaaaaatac taaactcata taattaggag gataaatatg gagattataa
     2701 agggtggagt tacagtttca gaaggatttt ttgcttcagg gattcactgt ggcttgagaa
     2761 aaaataaaga aaaaagagat ttagctttgg tttattcaga tgttttatgt gatgcagcag
     2821 cagtagtaac tcaaaataaa gtaaagggaa atcctgttta tgttacacag gaacacatta
     2881 aaaatggaaa agcacaagct atcatagtaa atagtgctaa tgctaatacc tttaatggaa
     2941 aagaaggact tataaatgct tataaaatgg ctaaattcac cagtgataaa ttaaatatta
     3001 aagaaaatga tgttttggtt gcatctacag gtgttatagg aaagccatta aatatagatt
     3061 taatagaaga aaatatggat gaattagtga ataatctaag taaacaaggt catattggag
     3121 caagagaagc tattatgaca actgatatta taaaaaaaga aatagcagta gcaataatgt
     3181 atggagataa aaaaataact attggaggta tggcaaaagg ttctggcatg atacatccta
     3241 atatggcaac aacgttagga tttataacta cagatgcaaa tattgatgga gttttgctaa
     3301 aagaagcttt gaaaattgct gttgataaaa gttttaatag agttagtgta gatggagata
     3361 caagtactaa tgatatggtg atgattctag caaatggaaa agcaaaaaat gatagaataa
     3421 acaaaaaaga tgaacattat caagtatttt tgagtgcact tacatatgtc tgtatagaac
     3481 ttgctaaact tgttgcaaaa gatggagaag gatgtacaaa actaattgaa tgctatataa
     3541 atggagcatt aagtgaagaa catgcagtaa aattagcaaa aacagttata tcttcttcac
     3601 tagtaaagac agctgtattt ggagctgatg caaattgggg cagaatattg tgtgctttag
     3661 gttatgcagg tgaagaaata gatatggaaa aagttgatat tatttttgag agtatgaaag
     3721 gatatattga agtatgtaaa aatggaaatg gattagattt taatgaggaa aaagcaaaaa
     3781 aaatccttga agatgacgaa ataagtatat tagtagattt aaacatgggc aatgccagag
     3841 gaaatgcatg gggatgtgat ttaagctatg actatgtaag aataaatgga agttatagaa
     3901 attaggagga tatactttaa tgattaatat agaaaaagca aatacactta tagaagcttt
     3961 accatatata gaaaaacatc agggaaagac tattgtagtt aagtatggtg gaagtgcaat
     4021 gaaaaaagat ggtctaaaag aatctgttat ggaagacctt gtgttaatga gttatgttgg
     4081 aatcaatata gttttagtac atggtggagg ggcagaaatc aataagatgc tagcaaaagt
     4141 ggatatagaa agtaaatttg taaatggtct tagatataca gatgaagaga ccatggaaat
     4201 tgtaaaaatg gtgcttgctg gaaaggttaa taaagaccta gtaaataaaa tacatacaaa
     4261 aggtggaaaa gcagtagggc tttgtggaat agataataat atgattttat gtgacccata
     4321 taaaaattat gaacttggat ttgtaggaga aatcaaaaaa gtaaatgttg agcttataga
     4381 atcttgtcta aagtcaggtt atatttcagt aattgcaact ataggtgttg gagatgatgg
     4441 agagacttat aatataaatg gtgacacagc agcttctgct atagctaaag agttaaatgc
     4501 agataaattg atacttctta cagatgtccc tggtttgtta agagagccag atgaagaaaa
     4561 atcattaata acagaagtta tattagagga tgtcgataaa ttatttgaag aaggtataat
     4621 tactggaggt atgataccta aaatacaggg atgcgtagat gccttaaata acggagtaaa
     4681 tagggtacat attttagatg gaagagttcc acattctata ataacagagt tatttactga
     4741 tagtggaata ggtacattaa taagaaaaga aaatgaataa aattataagt ttaagagggg
     4801 agaataagaa tgaataacaa caatacaatt tcaaaatgga acgaatattt tatagataca
     4861 tataaccaac ctaattttgt catagattat ggtgaaggaa gttgtttttt tgatactaat
     4921 ggcaataaat acatagattt tacaagtgga tatggagtat cttctcttgg gtatagtaac
     4981 ggtaatttaa agaatgctct aaaagagcaa gtagataaat tattacatac ttcaaattta
     5041 tattttaatg agccagtgtt gtattcagga gagaaaataa ttaattcaag tggaatggct
     5101 aaagtatatt tttgtaattc tggaacagaa gcaaatgaaa cagcattcaa aatagcaaga
     5161 aagtatagta gtgataaata tggtaatggt agaggaacta tcataagttt aaaagactct
     5221 tttcatggta gaacaatgat gtctttaatg gcaactggta tggacaaata ccataagtac
     5281 ttttatccac ttcccgaagg ttttaaatat atagaaagaa ataatattga agatttaaaa
     5341 aataatattg attcgacagt ttgtgcaata attcttgaag cgattcaagg cgaaggtgga
     5401 gttaatgttc ttgaaaaaga ttatgttttg gaactagtaa aaatatgtca agaaaaggat
     5461 attgtagtga tttttgatga agtccaatgc ggtataggaa gaactgggaa actttttggc
     5521 tatgaatatt ttgatgtaaa accagatata gttaccgtag caaaagggtt gggagcagga
     5581 ataccagtag gaggagtact agtaaataaa aaactttcaa aagtactagg aaaaggagac
     5641 caaggtacaa cttttggagg taatttatta gctatggtgg caacttcagt tgtattagat
     5701 gagatatcta aagatggttt ttataatgaa gtattagaaa aaggaaatta tataagaaaa
     5761 tctattgaaa gttttaataa taaagttgta ttaaagacaa aaggtattgg tctaatgata
     5821 ggaattgaaa ctaatattga atctagtatt atagaagaga aagctagaaa aaaaggattg
     5881 cttatcttga ctgctggaaa aaatgtattg agatttttac caccattaac aatcagttat
     5941 aaggaaatag atgaggctct tgaaatccta aaagatatat tactagagat taactaaata
     6001 ttcgaaaaat agtttaatta aagacaaaaa ttagaagtaa ataaaaaagt aaagtgttaa
     6061 caagtttaat tgaatttaaa aagtttataa aaaggggcga attaatatga ataatataag
     6121 tttaaaaggg aagagttttt tgacattaaa ggattttaca aaagaagaga ttagatattt
     6181 attaacttta tcaagttatt taaaaactaa gaaaaaagtt ggtataaaag gcgatttgct
     6241 acaaggaaaa aatatagctt tattatttga gaaaacatca acaagaacta gatgttcatt
     6301 tgaagtagca gctcatgatg aaggagcaca tgtaacttat ctaggtccaa atgattgtca
     6361 tatgggtaaa aaagagtctg tatcagatac agcaaaagtt ctaggaagat tctatgatgg
     6421 aatagaattt agaggcttta aacaagaaac agttgaaagt ctagcaaaat attctggtgt
     6481 acctgtttgg aatggactta cagatgaata tcatccaaca caaatactgg cagacttttt
     6541 aacaacaatt gaaaatgtag ataaagattt gaataaagtt aaatttgtat atgttggtga
     6601 tgctagaaat aatatgggaa actcactgat gataggatgt gctaaaatgg gaatccattt
     6661 tgtagcacta gcacctaagg agctttggcc aaatgaagag ttagttaagg aaatgaagga
     6721 aatagccaag aaatctaaag gagatataat gttaactgaa aatattgatg atgttaaagg
     6781 tgcagatgtt atatatacag atgtatgggt ttctatggga gaagaggagc aatatgaaac
     6841 aagaataaat caacttaaag attatcaagt aaatatggat atgataaaga aaactgaaaa
     6901 tgaaaatgtt atatttttac actgtcttcc agcatttcat gatttagaaa ctaaggttgc
     6961 tcaggagatt tatgaaaagt tcggattatc agaattagag gttacaaacg aggtatttaa
     7021 ttcaaaatat tcaaaggtat ttgaagaagc tgaaaataga atgcatacaa taaaagctgt
     7081 tatggtttct actatgactg atgtaaatat gaacaataat ttaatataga catgttttaa
     7141 tatggatttt actttaatga aaatttataa aatattttaa atatattatt caaaaactct
     7201 cattatatgt taaaataact tatgtatcaa caaattaaga gggaggattt gagatatgaa
     7261 aaaaatcata ttagctttaa caactttttt attagcagta tctttaacag catgttcaag
     7321 tgcaaaacca gaagatacaa tcgatagttt ttttaacagt gcgaagaagt ttgattttga
     7381 aggcatgaat aaggtaatgg aaaataatga caaaaaatat aaggatatat taaaggaatt
     7441 agatacgaaa gacccaaatg cccaatatgt tttagattat ctaaaacaga atgcatctaa
     7501 aatcacatat acaataaaag acagcgaggt taaaggtgat aaggctacaa tcaaagtaga
     7561 atgtaaattt atagattcaa caccattact tcaagaaata gtagctgagg catttacaaa
     7621 gatgcttggt atgagttttt caggacaaga cttaactgat gagaaaacaa ctgaaatgtt
     7681 agtatcaatt atgaaagaaa aacaaaaaag tgttaaagag acttatgtta caaaaactgt
     7741 agagtttaaa tgttctaaaa aggataataa atggataata agttcagtaa atgatgtagt
     7801 agctgatgta ttgttatcta atcttgttac agcaggacaa gaattttcta actctatgaa
     7861 tgcacttgaa taatattgtt taggtgtagt aaagtaataa atatatgatt atttttataa
     7921 ataaacatgt aaatatttat gaaggatact atttttggta tcctttttat taatatactg
     7981 aaatacatgg ttaatttcta tattgtcaag aaactatgat atatgttata agtctatact
     8041 atttaagata aacttataac atatattttt tatagtatag tgcatgaaat ttatttaact
     8101 aattttagat atttattgac aactgattca gccaagcatt ttgatgaatc aacaatattt
     8161 attgaaggat atgatttttc taaaactaca tttaaatcag tacaagcgat tactatattt
     8221 tctatagatt catttttaac atcttcaata agtttattcc aaatatctat attatgtgga
     8281 ttttctttat ctatttttat attttgaata agagtgttta gttttatttg ccattcatct
     8341 ttaaatataa attcatgtcc attatgtatt ataccattct ggtaaattgt agactcaaaa
     8401 gttgaactag tcgaaaaaag agttactttt tgtgagagag taggtaattt tttaactgtt
     8461 tcttctacaa tatttaatag tggtatatta atagactctt ttaattcttt aaagtatata
     8521 tgtgctgaat tgcatggcat tgcaataaaa tttacaccag ttgattcaag cttttgtaat
     8581 ccatcaatta tagtttcttt cataagttca tgatttattg gacggtcaat ataaaatggg
     8641 gtaggtaatg aatatatcat catctttgga aattcatcat catattttgc tccatattga
     8701 gattgacact catcaataat taaatcaaca aaaggggctg ttgaccttgg acccattcca
     8761 gccaaaatac caattaattt ttcattcata aaaattatct cctttacata taaatatatt
     8821 tatgtataaa ataaaaatct ataaaagcat aaaattatat tttataatag attatattat
     8881 acacttgtat aatataaata taaagtgagt ttttaaatta tatattttaa tatactttct
     8941 tatataaaaa ttattttaaa tcaagattat ctagtatttt taaaaatatt tttacagcgg
     9001 tatccaaatc atctacagaa acactttcat taggattgtg acttacaccc ccagcgcatc
     9061 ttataaatac cataccaata tcagttatat tatccatttc ctgagcatca tgaccagcac
     9121 cactataaat ataaaaagga ttaagattta aatctataaa acttttttct atgatttttg
     9181 ttattttatt agaacatggt acaggaacat tttcaaaagc aagttcactt gtataattaa
     9241 gttttctatt ttcacatata tgtgatattt cattaaatat cttttctaca gaattatcga
     9301 gaatttcttg tgaacaagag cgtacatcaa gagtaaattc agctctttca ggtaccacat
     9361 ttgatgaaga tggatagaaa ttcatctttc ctacagtagc aaccaaatca gcagtcgttt
     9421 tagctacttt ttcgatagct aatacacatt cacaggcaca acatccagca tcaagtctca
     9481 tattcattgg aagtgtacca gcatgtccag acttaccatt tactgatatc ttaaaacttt
     9541 taaatcctgt aatacttgat acaattccag caggaagaga ttcattttct aaaacaggac
     9601 cctgctcgat atgaaattct aaatatgcat ctacatcttt tctaggatgc aaatttgtta
     9661 agttttttgt attaaatcca aactttgtta cagcttcttc aagtgtaatt ccattttcat
     9721 caacagattt aagaagttcc tctttaaaag taccagcaaa aactttactt cccattaaag
     9781 aagtttcaaa tctagttcct tcctcttcag cgaaagcaat tagcttaata ttgaaaggat
     9841 aagacttatt attgtgtttt gcttcttcaa gaccaactag aggcactatt atacctaaca
     9901 taccatcaaa tatacctcca cactttacac tatcttgatg agatgcaact actaatgtgg
     9961 gagcatttgg aaagcttgat ttatatgtac ctataacatt tccaatgtca tcaatttcaa
    10021 tatcaagatt taaacctttc atccatgaac taattaaatc aatagatttt ttatgttctt
    10081 gagtcaagat aagtcttgta agaccatttg ttgttgaact tatttttcct aatacttgta
    10141 gataatccat acattttttt cctaacatat ttacctccta aaaatataat ttttatttaa
    10201 gctaagatta gcttttatac ttataatttt tgaactttca aataattttg tatgtatagt
    10261 aaaatactat tttagtatta ataatgatat aagaacataa agtctgtaga cgaagagcag
    10321 aagttttaag ttaaaaaata ttaaatgtga tttttattat tatagtattt ttctaaaata
    10381 atatctcttt ccagttagag gataattttc aagtgaaaat actttatggt atccaaactt
    10441 ctcataaaat ttaggtgctt gaaaactgaa tgtatctaaa aaaacatatt tacaagctcg
    10501 ttctttagca attttttcag ccttatataa aagttgactt ccagtacctt tatatctaag
    10561 cttctcacta acccataagt attttataga aagccaatta ccatgagtaa cacctataaa
    10621 acctgcaaca attttacctg ctttatctcg aatatatatt cctaaatcca ctggattttt
    10681 atcttcaagt ctagcaagat tatattttaa taatccttga aaaatagtat cttcatcttg
    10741 tttttctatt ttttctgtaa tttctaattc catatttgaa ctttctcctt gtataatctt
    10801 ttgcatattg gtatttttca ataaaatcag tagaaagaat atagtttaac attattctaa
    10861 tagtcattta tgttaatgtc aaactaataa tacttattct taattttata ttgttgatat
    10921 taaatttttt aaaataaaaa tgtagtttaa gttatataaa aagaaagcat caataaattt
    10981 agtaaaccta aattgttgaa tattaaaata agttaattta atacat
//
LOCUS       DAEMAE010000123        10992 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEMAE010000123 DAEMAE010000000
VERSION     DAEMAE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.125"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(62..586)
                     /locus_tag="KPH93_002452"
     CDS             complement(62..586)
                     /locus_tag="KPH93_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG1689259.1"
                     /translation="MKSVVVIYKSKYGSTEKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIVLGGGLYACNIAGISFFKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPVEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            complement(635..1132)
                     /locus_tag="KPH93_002453"
     CDS             complement(635..1132)
                     /locus_tag="KPH93_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1689260.1"
                     /translation="MDAETLMKKFMEDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAITLEKKGFIQIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRDMQEKVLKLLFEDFTEKEVEQLFFGIMKLSTGIENVKNYVEN
                     SDVGE"
     gene            complement(1374..2651)
                     /gene="hflX"
                     /locus_tag="KPH93_002454"
     CDS             complement(1374..2651)
                     /gene="hflX"
                     /locus_tag="KPH93_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG1689261.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEELLNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNKELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLTNNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DKILDSIDKEGLFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVASYFNEN
                     TSVINTEYREDGALLDVECSNIEYNKYKKYVLE"
     gene            complement(3368..5200)
                     /locus_tag="KPH93_002455"
     CDS             complement(3368..5200)
                     /locus_tag="KPH93_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689262.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLCLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEDQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEENEAM"
     gene            complement(5193..7439)
                     /locus_tag="KPH93_002456"
     CDS             complement(5193..7439)
                     /locus_tag="KPH93_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689263.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNIGI
                     QQGGVENSVPSVIREGELDKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLESNSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKKDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(7507..7968)
                     /locus_tag="KPH93_002457"
     CDS             complement(7507..7968)
                     /locus_tag="KPH93_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1689264.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKNGQSQRELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(8272..8838)
                     /locus_tag="KPH93_002458"
     CDS             complement(8272..8838)
                     /locus_tag="KPH93_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG1689265.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            9525..9863
                     /locus_tag="KPH93_002459"
     CDS             9525..9863
                     /locus_tag="KPH93_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689266.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            9885..10202
                     /locus_tag="KPH93_002460"
     CDS             9885..10202
                     /locus_tag="KPH93_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689267.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFASMVGVSPWYITQIESGKKNPS
                     LKTFIKFVSVLNISADILIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            10352..>10992
                     /locus_tag="KPH93_002461"
     CDS             10352..>10992
                     /locus_tag="KPH93_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689268.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKLKDNTELEK
                     IKMFKYKNGYIPNKLNLSLGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTK"
BASE COUNT         3760 a         1831 c         1169 g         4232 t
ORIGIN      
        1 tgtctaataa atttctatga aatataactt taaataataa ataattacaa atatatacct
       61 cttaaagttc tcttatataa tcaagtattg gagttaaatt ttctttatta caccaatcaa
      121 aggcttctcc agacaataaa ggtgcttcta ctggtttaag accttgacgt tcttttttag
      181 ctatagattt tttaaaaaaa ctacaaagag tataatctat aaaagacatt tttgtctcat
      241 cccatgcccc acgacaataa aaacaaggaa ctttgtttaa ttcatcctta aagtttactt
      301 ctcttaactt ctttattact tcatcatcat atggagcagc tcctacacca aatactacta
      361 gatttttttt ctgtaattct ttataatttt ttttaaagaa agaaatccct gctatattac
      421 aagcataaag acctcctcct aaaacaatcg tatcatactt cataaccttt tcaatattaa
      481 tattcttagt ttcaaaaata tcacaagaca ataattctga aagccattta gcatattttt
      541 ctgttgaacc atacttactt ttataaatca ctactacact tttcatataa attactccct
      601 ttctgatgtc atacaaagtt gatttgacac ttgcttattc tccaacgtca ctattttcta
      661 cataattttt cacgttttca attcctgtac ttaatttcat aattccaaaa aataactgtt
      721 ccacttcttt ctctgtaaaa tcttcaaata aaagctttaa tactttttcc tgcatatctc
      781 ttactttttt agaatactct tccactctat ctttcgtcac aagacagata gctcttgaat
      841 ctttttcatt ttgctcaatt tgtataaaac ctttcttttc taaagtaata gctagttttt
      901 ttacattttg tcttgagcac cccattaatt ctccaagttt ggttaatgta agagtttcat
      961 ctacagagtc cgcaatagct aaaagtaacc attgtttcat tgttaattct gggtctaatt
     1021 tatcacatgt agtttgaagt ttattttgca ttacaaatat acttgcaaat atagcttgtt
     1081 ttctactatc atcatcttcc ataaattttt tcattaaagt ttctgcatcc attattatac
     1141 acttctcttt atcttagatt ttaatttttt gataacttta aatagtaact agttacttta
     1201 tattatagta attagttacc attgtgtcaa taaatttatt tttattataa atggcaaaac
     1261 ggcatagctt tatggctata ccgtttcata aaatcatact ttacaataat tttataattt
     1321 attataaaat tataaattaa atgtatttta agctataata attttttcaa aacttactct
     1381 aaaacatact ttttatattt attgtattct atattgctac attctacatc taaaagagct
     1441 ccatcttctc tgtattctgt atttataacg ctcgtattct cattaaagta agaagctaca
     1501 tgacctttgt catatggaat taagaactta catcttacat aatctttaaa tatgctatca
     1561 caaatacatt ttatcatttt atctatattt atatcttttt taactgaaac aaataggcct
     1621 tctttatcga tactatctaa aattttatct aatacttcta catctattaa atcaatttta
     1681 ttatacacat gtatcatagg tataccatct gcacctattt gttttaaagt atcttctgtc
     1741 actttaatat gacttttata gcttggattt gaaatatcaa taacatgtaa cagtacatct
     1801 gcctcacatg cttcttctaa tgttgaccta aatgctttta ctaagtcatg tggtagatta
     1861 cttacaaaac caacagtatc agataataaa aattctttgt tatttgtaag aactatattt
     1921 ctgacagaag tttcaagtgt agcaaataac atgtttttct caaacacttt cttttcttca
     1981 gagtttttaa atatatctac cagtttattc attattgaag atttaccagc atttgtatac
     2041 ccaactagag ctacatttgg aagatttgat tttcttctca tacttctttg agtttctctt
     2101 tggaatttta aatcatctaa ctccttattt aaagatgtta tcttttcttc tattcttcta
     2161 cggtcaagtt ctaatttttt ctcaccagaa cctctatttt tagtaccaac tccaccactt
     2221 tgacgtccta aattttcatt tgaacctata agtcttggta acatatattt taaacttgct
     2281 acttctactt gaagtttagc ttctctagtt tttgcccttt gtgcaaatat atcaagtatc
     2341 aatgctgttc tatctataat ttcacaatta agcttttctt caagattttt tagttgtgat
     2401 gtagaaagtt cattgtaaaa tataactatc tctatgttct ctttctttat caagtttaat
     2461 agttcctcaa ctttacctgt cccaatgtaa aatgctctat taacttgctt tgcattttga
     2521 gttatactcc ctacaacatc aatatcacat gctgaacata agttttcaag ttcaatcatt
     2581 agttcattaa agtcctcaga tttattattt atgtttaatc ctactgttat tccttttttc
     2641 gccatatcca tattctcctt atctgctatt ttttatttaa tctatagcac gagattatgg
     2701 ctctataatt aaattcatca agaattttac ttttttctta tccttaaatg acacttctta
     2761 tttaccatta taataaccat aatcgtgcat tattttctca atcttatata aaaaatcact
     2821 ttcttacacc tcctcccaaa tatattatgc agtttttcaa caaaaaaact ccttacaaat
     2881 ctaagtaata caaaacaagc tacttatatc ataagaagga ttaaagttct aaatatatat
     2941 taaaaataca ttagtttaat taacttacat gtaattatca ttttatattt aaccctattc
     3001 ttaaatttaa gtagttattt gcatgattaa taaaagtaat aaaaatatat acttcaatat
     3061 aatcatcaac tattaaaatt aaattctatt tataataaaa tttaaagaat aaaattaaat
     3121 atacattttg ataatcacct ccaacatttt ccttactaca tattataata aaatatacac
     3181 tttgtaaaga cataaaatta tgtggctatc tcaaaatata gagaatcttc tttttcattg
     3241 ttaaaatgat aaaaatacat tttatttcat acacaaaaga gtgccttatg aactaaaaag
     3301 ttcattttaa gacaccctca tttattttat cttattaatt gtatgtctat gctaagatag
     3361 ttgtttgtta cattgcttca ttttcttcaa attgagaatt gtaaagatta gcatagaatc
     3421 cgcctttctt tagaagttcc tcatgatttc cttgttcaac tatatcgcct tcattcatta
     3481 caagaatcat atcagcatca cgtatagttg ataatctgtg ggctataaca aaacttgttc
     3541 taccttccat aagattatcc atagcttttt gaatcaatac ttctgttcta gtatcgacag
     3601 aacttgtagc ttcatctaat attaaaattt taggatcagc taaaatagca cgagctatag
     3661 tcaataattg tttttgacct tgagaaacat tacttgcttc ttcatttaat ttcatcttat
     3721 atccatctgg taatgtctta ataaatctat gaacatgtgc agatttagca gcctctataa
     3781 cttcttcatc tgtagcattc aacttaccat atcttatatt ttccataatt gaaccactaa
     3841 ataaccatgt atcttgaagg accataccaa acatttctct aagttcacta cgattaaaat
     3901 ctttaatatt atgaccatca atcagtattg aaccgctatt aacatcataa aatctcatta
     3961 aaagtttaac tattgtagtt ttacctgctc ctgtaggtcc aacaatagca actttttgac
     4021 caggtttaac atttactgag aaatcattta ttatagtctt gtttgggttg tatccaaagt
     4081 tgacattttc aaaatcaatt ttaccttcta aaccatctat tgaaactgca ttttcaacaa
     4141 tttggtcttc ttcttcctct tctaaaaact caaatactct ttcagaagct gctgctgttg
     4201 attgcaataa attggcaacc tgtgcaatct gagtcattgg ctgtgtaaag tttcttacat
     4261 attgtataaa tgactgtatg tcaccaacct caattgtttt cttaattgca agccaacctc
     4321 ctaagattga caccattaca tatcccaagt ttccaataaa catcataagt ggttgcatta
     4381 tacctgataa aaattgagac ttccatgctg aattatacaa actatcattt aacttatcaa
     4441 actcttcaat tacttcatct tcacgattaa atgctttcac tattgtttga ccactgtaag
     4501 tttcttcaac ttgaccattt acatttccta aatattcttg ttgatatcta aagtattttt
     4561 gtgatttctt tattattctt gaaattacaa acattgatat tggtaatatt aaaactgcca
     4621 ctaatgtcat aattccactt atagataaca tcattatcaa aacacctatc atagttgtta
     4681 ctgatgttat aagctgtgtc atactttgat ttaaactttg atttaaagta tctatatcat
     4741 tcgtaattct cgataatatt tcaccatgcg tcttagtatc aaaatacttc attggcaatc
     4801 tatccaattt tgctgaaatc tcttttctta aattatagga tactttttga gaaattccac
     4861 tcattataaa tccctgtata aaagagaaca gtgcacttat caaataaaga caaagtaaaa
     4921 aggttaaaat ttttccaatt gcattaaaat ctatacctac attccctcca gaaactttac
     4981 tcactaaccc ttcaaatatc tttgtagttg cctttcctaa aatttttgga cctactatag
     5041 aaaacgatgc actaccaata gcaaatacaa ttactagaat tattgatatt ttaaatttag
     5101 ataaatatgc aattagtttt cgcatagtcc ctttaaagtc atttgctttt tgtcctgctc
     5161 ccatagattt tcccatagga cctcttccca ttctactcat tagctagctc ctcctttgaa
     5221 agttgtgata aagctatttg ctcataaact tcgcaatttt ttagaagctc tttatgtgtt
     5281 cctattccaa ctatttctcc ctcatttaaa acaattatct gttctgcatt taagatagtg
     5341 ctaattcttt gtgcaactat tagaactgta ctttcagcag tttttgattt taaagcctta
     5401 cgaagagctg catcagtttt taagtcaagt gctgaaaaac tatcatcaaa tatataaact
     5461 tctggatttt ttgctattgc tcttgcaata gaaagccttt gtttttgacc tccagataca
     5521 tttgttccac cttgagaaat ttcagtttta aaagcttccg gtttagaatc tataaactct
     5581 gtagcttgag ctatttcagc tgctgttctc atttcttcat ctgttgcgtc ttttctacca
     5641 tattttaagt tggattctat agtaccagaa aacaatactc ccttttgagg tacatatcca
     5701 attttttccc ttaaatcatg ttgagaaaca ttttttatat taactccacc aagtaatatt
     5761 tctccttcag taacatcaaa gaaacgagga attaaattta taagagttga cttaccacta
     5821 ccagtacttc ctataaaggc agttgtttct cctggttttg ctgtaaaaga tatatttgtc
     5881 aatataggct cttctgcatc tggatacatg aatgaaacat ttttaaattc aacaagaccc
     5941 tttttatctt tcttaaactc ttgtgcctct gatttttctg ggtcttttat gcttatatct
     6001 gacactaata cttcatctat acgtgtagct gaaacggatg ctcttggaag catgatagat
     6061 accattgata tcattaagaa tgacattatt atttgcattg tatattgtat aaatgccatc
     6121 atatctccaa cttgcattgc tccagaatca acactatatg cacctctcca aactataaga
     6181 actgtgataa ggttcattat aagcatcata gcaggcatca tacatgtcat aactctattt
     6241 acaaaaagat ttgttttagt taaattttta tttgctttat caaatctttc ttcttcatgt
     6301 ttttcagtac taaaagctct gataactgga ataccagtaa ttatttcacg acttactaag
     6361 tttaacttat caacaagctt ttgaactagt ttaaacttag gcataaccac tgagaataac
     6421 actacaatta atgataaaat agcaactact gctactgcta taatccaggc cattgaagta
     6481 tttgtgttta aaacctttat cactccacct atagccatta tcggtgcata gaatactatt
     6541 ctaagcaaca taaccatcaa catttgtatc tgttgaatat catttgtact acgagtaatt
     6601 aatgacgctg tagaaaattc attcatttca gtatttgaaa atgacataac ttttctaaag
     6661 actccacttc ttaaatttct acctacactt gctgctactc ttgctgctaa aaatcctact
     6721 gttattgtag ctaccatact aatcattgct ataccaagca ttttagcacc agtaaataat
     6781 atataattat tttgagtttt ttctgtatca atacctattt tctcatattc tgaccttaca
     6841 tatgaaaccg agctttgtgt tatcatactc tctggcatac tttcaaagcc tttgctcatc
     6901 tctttagtca atgtatctaa ctgttccttt ggcattgctg atagcaattt aaatatatca
     6961 acatctccac tttgagtagc ttgtggtgga agtttactca ttaactgagc tttcatagct
     7021 tttacctctt tactattgct ctcaagccct gatactatta aaatagcttt accaaaaata
     7081 acatttaaat catctatagt gtctttgtct ttagtattta actcataaag agattcatct
     7141 tttagacctg ggtacttatc tttatactta acataatcct ttttattcaa caaagtataa
     7201 ttatctagta ctttatcctt actactttta tccataaata aagtgatttt atctaattct
     7261 ccctctctaa tcacagatgg aacagagttt tctactccac cttgttgtat acctatattt
     7321 actatattgg atgtgtattc tggtaaagaa aggtcacata ccgcttgaat taccaataga
     7381 cagactatta ttactattga taatgccgat ttctttaaat atttaattaa tttaaacata
     7441 catttcttcc cttctgtaat attaattagt ctaataacat taaattgaaa aacattattt
     7501 taaaccttaa aaatatttct catcttcatg cttatgacat cctgctttta aattatctcg
     7561 tacttgcatt agaagtcttc taagtactat tttttcttct tcactaaatc ctaaaaaaaa
     7621 ctctctttcc aattccttgt taaattcttc aactttatta catatttcta aacctttgtc
     7681 tgtcaaaaaa atcctagata ctctttggtc cttttcatct tgttttcttt ccacaagtcc
     7741 agctttttcc atcctcttta tcataactgt agttgtagat gcttttactt tccttttatc
     7801 tgctaattcc ctttgacttt gcccattttt catctttaaa gaaaaaagta tttgtggttg
     7861 accatgatat aagcctaatt tatcaaaata aaaatgagtt cttataaaat atattcgcat
     7921 taattgcaag aaaacatcat ttaatttttc ttcagaattg ctattcatac ttaccctcct
     7981 tatttttata ttacttagtc ggctaagcat tattttacta caccatatac attttttcaa
     8041 tatatttata aaaaattcat ataaatttca tttattaaat attttcacat catatagtta
     8101 taattttaat aaataatagt aagattttta attagtcagc tacttatatt ttttattgta
     8161 aagcttaaaa taaacctttt catatatttt aaaataaaaa agttgtccca aaataaattt
     8221 tgaaacaaca tcttacttga cagtatcttg attatataac attataatac tctatttatc
     8281 ttcgtatcca tttacaacaa catctaactt cccagtttct ggtgatataa ttagaccatg
     8341 tacaataata ccatctggaa ttaaaggatg cccttttatt aaatcaatac tatcttttac
     8401 agattcttct gcagaatcga atccatgcaa ccattgtttt gtatcaattc cagcactacg
     8461 aagtgtaagt attacatcat tagatattcc tctatctaaa attttaccaa gtaacttatc
     8521 cgtatcaaca ttgcacatac cacatccatg atgccctaca accataacct catctacatc
     8581 aaattcgtaa attgcaacta aaacacttct cataatactt ccaaaaggat gcattatagt
     8641 agctcctgca ttttttatta gtttcacatc tccatttttc aaattcatag ccttaggcaa
     8701 aagctcagtt aatctggtat ccatacaaga taagactact atttttttat ttggatgttt
     8761 tgatgtcaca tattgttcat attctttgtt ctctacaaat gatttattat actctaatat
     8821 ctcttcaagt tttctcatat gtaatatcac cctctttaaa aattttttta ttattatatc
     8881 actattaaag tacaatatat acacatataa tttaatttat atttattaaa taattttata
     8941 agattattac tctactataa atcttatata taacttgtat ttataagtat ttgtatattt
     9001 attttttata atctaaactg atattatata ttttcattat ttttttaatt ataatttttt
     9061 taaaatttta ttctaaatac cttccctata attaattttt tatttcacct ctatgtaatt
     9121 agatttaaaa atatttacct taccaatata atgattaaac tcaaaaggct tatcgaaaaa
     9181 ttttccatgt acataaattg ttcaatatat aaaatcgaaa atttttttaa ttttcaaaat
     9241 atattctaca tatctaatat gtaattgcaa taaaaaatgc tataattaac atattattaa
     9301 ttcgttttat tattgtagta tagctatgaa attatgaaaa tatttctcta attaaaaata
     9361 tgatttatcc atatttgttt attgtagata cagactatac aaaaataaat aacacttatt
     9421 tcagtataaa aacatatata gtctatattt agactaaaaa agtataaata gtctatataa
     9481 aacaacttaa actatgaata taatctaaag tggagggata ataattggca aatataggaa
     9541 aaataatagg agatagaata aaagtctata gaacaaaatt aggatatagt caagagtttc
     9601 tagcagaaaa ggcaggttta catccaacat atattggtca gattgaaaga gctgagacaa
     9661 atacatctat caatattata atgaaaattg ctactgcttt agatatacca cttgaattgt
     9721 tatttgcaaa tattattaca acagaaggcg ttgataactc tgtaccttta gaatgttatg
     9781 aaatgattaa taaattaact gaaaaagaac aaattgcaat gctagagtta ataaagtgca
     9841 tcattaaata caaagaaata taagtgaggg aaatagtaac aataatgaat tatataggta
     9901 aaagacttaa agaagaacgg aaaaaagcaa atttaacttc aaaagaattt gcaagtatgg
     9961 ttggagtatc gccttggtat ataactcaaa tcgaaagtgg taaaaagaat ccaagtctta
    10021 aaacattcat aaaatttgta agtgtattaa atatttctgc agatattctt ataaaagata
    10081 ttacttccac tggaaagaca tatttagaaa atgatataaa tgaagagtta aaagacttaa
    10141 attcaagaga actaaatctg atatcacaga ttatagacat cataaaaaag aataaaatat
    10201 aattctttat attttgtata aaaaaaaagg ttatttgaaa taaataacct ttttttgtct
    10261 ttttgtgtta acattgtctt atagtgctaa tgagtactat tagaccatgt tctatggcct
    10321 tattttaata taaaacgatt ggagataggc tatgattgtg attgaaggta gcgataaatt
    10381 taagattgca aaagaatata ttgatgtaga atatactctt tttagcaaag taacttttag
    10441 gtatgaaaag ttgaaactta aagataatac tgaattggaa aaaattaaga tgtttaaata
    10501 taaaaatggc tacatcccta ataaattaaa cctttcttta ggatatggat tctcttctta
    10561 taaaaagcaa ataattagag aaactgtaga tactttaaga ttgacagaaa ttttttcaag
    10621 cgagaacata gaagatatta aatttataaa agatggtaca aaaaaattag aaattagcat
    10681 agagaaagtt gtgaaattta aacgtcgaaa aaaaagaaat tatgtttgtt gctattgccc
    10741 tgatatgtat agagacataa aactcgacaa agaatctatc aataagatat acaacagaaa
    10801 aataaaaata gaaagagaag ttaatatttt tgaagatgag gatgttataa taaacaaaaa
    10861 agtattgaag tttccaaagt cttggacaaa gaatatgcaa aaatattggt taagtgaaaa
    10921 taagtatccc atacattcta ctgtaattga tgatgataga tataaatgtt gtaatgtaca
    10981 atatacaaaa aa
//
LOCUS       DAEMAE010000124        10779 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEMAE010000124 DAEMAE010000000
VERSION     DAEMAE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.126"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..1094
                     /locus_tag="KPH93_002462"
     CDS             246..1094
                     /locus_tag="KPH93_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG1689269.1"
                     /translation="MNNQKNLNIPITISSYTLGTDVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1173..3095
                     /locus_tag="KPH93_002463"
     CDS             1173..3095
                     /locus_tag="KPH93_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1689270.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVLGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPVLGHEAELKSAEISKEVLVVGGGVGGML
                     AAWICAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDAVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDNGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3177..4040
                     /gene="aroE"
                     /locus_tag="KPH93_002464"
     CDS             3177..4040
                     /gene="aroE"
                     /locus_tag="KPH93_002464"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG1689271.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNTKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4240..5448
                     /locus_tag="KPH93_002465"
     CDS             4240..5448
                     /locus_tag="KPH93_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1689272.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYGKEKVYD"
     gene            5693..7558
                     /locus_tag="KPH93_002466"
     CDS             5693..7558
                     /locus_tag="KPH93_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG1689273.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLILSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEYGYKDAKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKVEFEKEINDILLSEKWKNMYFEALRDASGKTEDMIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEERFKVDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKMVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSKIKLIN"
     gene            complement(7733..9097)
                     /locus_tag="KPH93_002467"
     CDS             complement(7733..9097)
                     /locus_tag="KPH93_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1689274.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGISVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKEQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9275..10618)
                     /locus_tag="KPH93_002468"
     CDS             complement(9275..10618)
                     /locus_tag="KPH93_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG1689275.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10691..>10779)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         4021 a         1364 c         1838 g         3556 t
ORIGIN      
        1 aatagaagaa tacaagaatt atcttataaa taaaatctat aaacaaaatc tatgaattaa
       61 taggaaatta tcaatttgtt attagtaaag tctcgtgata caataaattt ataaaatcta
      121 gataaataga aatcaaaaaa tacttttatt tttaaggcaa aataatacta ctagtaggat
      181 aaataaaaat ataaaatcaa aaatactatt aattataaag aaaataaagg gggcaaattt
      241 aagctatgaa taatcaaaag aatcttaata ttccaattac aattagttct tatacattgg
      301 ggacagacgt ttcattccgt gatagagttc gtattgcaaa agaagctgga tttgatggta
      361 ttggattgcg tgctgaaaat tatattgatg caaaaaatgc aggtgttaca gatgaagaga
      421 tgattgcaat tcttaatgaa tacgatatga aagttacaga agtagaatat ataactcaat
      481 ggggaacaga agaagataga acggaagctc aaagagaaaa agagcaaaat gtttatcata
      541 tggctcactt atttaatgtt aaacatataa attgtggatt attggagatg ataccagaag
      601 aacaaattat aactgcattg ggagaattat gtgacagagc aggtgaactt attataggtc
      661 ttgagtttat gccatatagt ggtgtaccag atttggctac tgcatggcgt gttgtgaaag
      721 gatgtaatcg tgaaaatgca atgcttatat tagatacttg gcactgggct agagcaaagc
      781 agacagcaga ggaccttgca cctgttccag cagataagat agtatcaatt caagtgtgtg
      841 atgttttaga aactccttat gaaaaactta gggatgaatc actacatgac cgtttggctc
      901 caggtgaagg atatggaaac acagaggaat ttgtgaaaat aataaaagaa cacaatatat
      961 caccaagagt cataggggca gaagtaatat ctgattctct aatatctaaa gggttatcta
     1021 ctgctgctga aactgtattt aatgcaacta aaaaagtttt ggataaagca tggtctgaag
     1081 tatctccaaa ataaacttat ataatataaa aatatacaga atattataat ataaaactct
     1141 taaatataaa acttgaaagg aaggaaaacc ttatgaagat tagttctatg tttacaccta
     1201 taagaattgg ttcaatgaca gttccaaatc gttttgtagt tcctcctatg ggaaacaatt
     1261 ttgcaaacac agatggtact ttaagtgaaa cttctaaggc ttattatctt gagcgtgctt
     1321 taggtggttt tggattaata actatagaat ctagtgttgt agacaaaaaa gcaaaaggag
     1381 gtcctcgtaa gccttgttta tatgatgata gcacaataga tagttttaaa aatgtaatag
     1441 atgcttgtca tgatgcaggt tcgaaggtat ctatacaatt acaacatgct ggttctgaag
     1501 gaaatgaaaa agtagcagga catccattaa aagctgcttc tgcaatacct gcatcaaatg
     1561 gtcgtaatac accactagca ataacaacag aagaaatata tgaacttata gaatcttatg
     1621 gagatgctgc attacgtgca caaaaagctg gagcagatgc tgttgaagtt cattgtgctc
     1681 atggatatct ggttagtagt tttatatcac aaagaacaaa taagcgtgta gatgagtttg
     1741 gtggatgctt tgaaaataga atgagattac ctcgtcttat cattgaaaat atacgtaaaa
     1801 aagtaggaaa ttctctagcg atattatgtc gtataaacag cactgatgat gtcctagggg
     1861 gaattgacgt acatgatagt tcagtaatag cagcatatct agaagattgt ggaatagatg
     1921 gacttcatgt ttcacgtagt atacatatac atgatgaata tatgtgggct cctacgactt
     1981 tacatgctgg attcagtgca gaattagtta ctgaaataaa aaaggctgtt agtataccag
     2041 taataacagt tggacgttat acagaaccac agtttgctga gttaatggtc agacaaggaa
     2101 gatgtgattt agtagcattc ggtagacaaa gtttggctga cccagaaatg cctaataaag
     2161 ctaaaaatgg aaagctagac gaaatgatac catgtattgc atgtttacaa ggatgtgtac
     2221 caaatatgtt ccaaggcaaa cctatagcat gtcttgcaaa tcctgtatta ggacacgaag
     2281 cagagcttaa gtcagccgaa attagcaaag aagttcttgt tgttggtgga ggcgttggag
     2341 gtatgcttgc agcatggata tgtgctaaaa gaggacataa tgtaacttta gttgaaaaat
     2401 ctgaagttct tggtgggcaa atgcgtttag ctgcatatcc tccgggaaaa ggagatatta
     2461 ctaacttagt tagaagttat atttcaaaat gtaatcaata tggtgtaaaa atatgtacta
     2521 atacagaagc tacagtagaa cttataaaag agaaatcacc tgatgcagtt attatagcta
     2581 ctggagcaac tccacttgta cttcctatac caggaatcaa tgattcagga ttaattcatg
     2641 ctgtagacct tcttgatggt aaaaaatctt gtgggaaaaa agtacttgtt gttggaggtg
     2701 gaatggttgg atgtgaagtg gcagccttcc ttggagaaca agaacatgag gttactgtta
     2761 ttgagcttag agaagaagtt ggtgcagatg taatttcaga acaccgtaaa ttccttatga
     2821 atgattttga aaattataat atacatacca ttactggtgc aaaagtttct aaattttatg
     2881 ataatggtgt cgactattta cttacagatg gcacagagca tgatttaaaa ggttttgatt
     2941 cagtagtact tgctatgggt tctcgtaatt acgacccatt aagtgaagtt ataaaagaag
     3001 ttgtaaaaga aacttatatt gttggtgatg cagtaaaagc tcgtagggca ttagatgcaa
     3061 caaaagaagc ttttgaagta gcattaaata tataaataat aaataaattt ttctttgtaa
     3121 ataactgaat aggttatttg caaagaaaat gataatattg ggaggagatt atagttatgg
     3181 aaatttcagg tagaacagga ttatttgcat taataggtac accagttggt cactctaaat
     3241 caccagtgat gtataattat agttttaaga aattagattt agattataga tatttagcct
     3301 ttgatatcac agtagataag gtaaaagagg cacttttagc aataaaaact tttaatataa
     3361 aaggagccaa tgttactatg ccatgtaaat ctgctgttac agaatatatg gatgaacttt
     3421 ctccagcagc tcgtataatt ggagcatgta atacaattgt aaatgacaat ggaaagttag
     3481 ttggtcatat aacagatgga gtaggctatg ttcgtaattt aaaagaaaat ggggtagaag
     3541 ttaaaggtaa aaagattaca attatgggtg ctggtggtgc agcaactgct atacaagtac
     3601 aatgtgcttt agatggagca agagaaatat ctatctttaa tactaaggat gatttttata
     3661 aaagagcaga gcaaacagtt gagaatataa aaaaagatgt accagaatgt gttgttaatc
     3721 tatatgattt agaagataca aacaaacttt atgaggagat agaatcaagt gatatattga
     3781 caaatgcaac tcttattggt atgaagcctt atgacaatga aacaaatata aaagatacta
     3841 gtgttttgag aaaagactta gtagtaacag atgtcgttta taatcctaaa aaaacaaaaa
     3901 tgattgaaga tgctgaagct aatggatgta aggctattgg tggattagga atgttattat
     3961 accaaggagc agaagctttt aatctatata ctggattaga aatgcctgta gaagaagtta
     4021 atgaattatg ttttaaatag ataagagtga tatcttaaac aatgaaaaaa catacgtatt
     4081 tcaatatttt taataataaa ttacaaattg tcatagttgg ttttgcatca tagtccagtt
     4141 aaatttgaag ctttaactga ctatacttta aatccattat ggggatgagt ttattgtaca
     4201 caaaaatgtt aataaattaa caagaatggg agaaaaatta tgaacaataa aaaatattat
     4261 cctactgcca tagcgttgta tttttcatat tttttacttg gtattggaat ttcaatttta
     4321 ggacaataca aaccagaatt ttcatcaatg tggggggcta aaacactatc agatggaaca
     4381 ttggatgtaa gtattgtttt agctgttatt gctgcactag gattagggag acttatatcg
     4441 tatccatttg caggacctat ttcagataag tatggaagaa aagtaagtgg tcttatagga
     4501 aattttctac atgcaatttt ctttgttgga attgtatttt caccaaattt ttatatagca
     4561 tatgtatttg caataattgg aggggcagca aattcattct tagatacctg tgtaactcct
     4621 tcttgtatgg agatatttgc tagtttagga acaatagcaa atatgtttac taaatttaca
     4681 atagctttag cacaattttt acttcccttt ataattggat ttgttgcagc aaattcaata
     4741 tcatttaaag ttatttttat agtaacagcg attcttattg tagtagatgc cattttgata
     4801 gctgtgttac catttccacc agctaataat gttattgata acaaaggcaa aacagttaaa
     4861 tcagagaaga tgaaatttac accaacaagt atagctcttg tttgtattgg atttacatgt
     4921 acatctacat ttgtattatg gttaaattgt aatcaggaat tgggtaaatt atatgggatg
     4981 gctgacccta caaaaattca atccttttac tcaatgggag taatatgtgc agtattaata
     5041 acttctttac ttattaaaaa gtatattaaa ccaataagaa tattagttat atatcctata
     5101 atagctttac ttatgctatt agttgtatat tttgttcaaa ctccaactat ttgtatgtta
     5161 gggggatttg taataggtta ttcagcagca ggaggtgttc ttcagttaac aacgtcaact
     5221 gcaaatgaaa tgtttccaac taataaagga aaaataacat ctatagttat gattgcatct
     5281 agtatagcaa actatgttat tttaaatata gcaggtatta ttacaaaatc agggggagtt
     5341 aatggaccaa aatatgttgt attgtttaat gtagcaataa catttgttgg aattttactt
     5401 gcattgtttg taaacatgag atatggaaaa gaaaaagtgt atgattagga tgtctaatat
     5461 tagtataata tatttataga gaagtccaaa tttaggatgg gtttctatta aaaataatta
     5521 aaattaataa ggatattatt tacagttata agatagggta tttacataat taataataca
     5581 gatattacta gaatttcaat ttctagtaat atttttttgc gtataaatgt aaacttgacg
     5641 aaataagtag tataatcaat ataaattaca tcatgtaaag gaggataatt tggtggagaa
     5701 tgtttttaag cgattacagg aatttaatgg ttatgatgga tacaaagaat catttgaaat
     5761 gaattaccta tgtatatatg aaagtatacc tttaagagag caagtagagc ttgcaaataa
     5821 tctagtagat gaaattttaa atatgtataa aagtgaatct aatgaaattt atttattgga
     5881 ggatagtaat tctaagtctt taatttgtta tttcgaaatt tttatgaaaa agataaatac
     5941 tttggtaaaa gaaatgatta ttgatgagaa atggttgtat aaattaacta aagaattaat
     6001 atataagagt aaaaaagttg agtatgtaaa actagggtta attttaagtg agaaatatct
     6061 aaatgtagaa aacttaagag aagttgtaga tacattttct aaatctggag aatacgtatt
     6121 ttatctgagt aatactatta aaaaacttga attttacaat acatatttat ttaatttatc
     6181 taaaaaggct acaggttcaa taaaagtatt tgctattgtt aatatggaaa atttagattc
     6241 taaaataaat tcctatctaa ttgaatatgg ttataaagat gcgaagtacg aacgattatt
     6301 gatgaattat attatttcaa ttgtagattt gaatgaatat cttgaaaaac gagatttaga
     6361 taaagaaaag ataaataatt tagcacgtct tatatgcaat tacttgctaa gtgtagaatt
     6421 taaatatata ggaaataaat tagaattagt gaatcgattt ttacctactg tagtaaatta
     6481 tgggactaac tttgaaagtt tatattcaat tttcctaata gctataaatg ttttaaaaga
     6541 tgaaaatata gagtgcaata aggttgaatt tgaaaaagaa ataaatgata tcttgcttag
     6601 tgaaaaatgg aaaaatatgt attttgaagc attaagagat gctagtggaa aaacagaaga
     6661 tatgataaaa atgtcagaaa tttatgatgt aaacttatct tttgatgact tgttaccgta
     6721 tttgaataga gatattagag attttgaggt atattggcat atatctaaaa aaggaacgac
     6781 aagttccaga ttaaaattgt taaatttttt tgaagagagg tttaaagtag atgatttaat
     6841 tggaaaaatg aaagatatag aaaaagataa attgactcaa gaatattatg atgatatgtt
     6901 attttttatt gtgcttaaag gaagtaaatc actatatcct gaaggaaaga atatatcttt
     6961 aaaaggaata tttggaaata taaatgaagt aagaaaagaa tccataaata ttttgaaaag
     7021 atatagagag aaattatctc ttgaagaatt aaaaatggtt aaagaagcct atgaaaaaga
     7081 aaaaaatgtt atattgaagg atgaattaag aagagtatta tatgaaagta acaatttaaa
     7141 aaaagaattt gtaaatattg aaaaaataaa agtagatgaa catggtaagg atatctattt
     7201 gacttcaata gccgttgctg gttcaaggtt tagaaataga gaatatcttg aaaaagaatt
     7261 ggagaagagt aagatatact atctaactag agagaaagat aatctatatg atgaaaaagc
     7321 tattaaaata gttggtgaaa ctggatatgt aataggttat gttccaagaa aagaaaacta
     7381 tattttgagc aatttattag atggaggaaa actcttgtat tgtagagtaa ctgaatataa
     7441 tttatatgaa gattgtattt atgcaaatgt atacttgagc tataaagatg taattgaaac
     7501 ggttgaaaac tctctaaaga tggttttaga taagagtaaa ataaaactta taaactaaaa
     7561 aatttataga ttacttagat gatataagtc taacagattg gttatttttc tggaattatg
     7621 atgtatttta tgagcaatgg taatgaatag aaaataaaaa aacaattgtt ttgtatatat
     7681 gatgcataaa tatatttata tattcatatt tacaaaacaa ttgaaattta tattatttta
     7741 tgattttaaa tttagatgga attttaactt ctgccttatc ttttatttta gaagcttgtt
     7801 ccttaacttt atttaacaat tcagattcat ctacaactaa tacttttctt tccttcatca
     7861 accatttacc atcacaaata gtagaatcta cattttcact agtcatagaa taaactatat
     7921 ttccaattgg attatgcatt ggcaaacaat gaattgtatc tggatttaga ataatcatat
     7981 cagctttttt tcctacttca agactaccaa tttctttttc taataaagca cattttgctc
     8041 catttatagt agccatttct aacacttctt cagctggtac aactgttgga tttaaagttc
     8101 tacctttatg tattaatgaa gtcaaataca tatccctcat aaggtccatt ctattatttg
     8161 aaggagctcc atcagtacca attgaaacag atattccttt ttctaacatt tcaggaattg
     8221 atgcaaatcc tagaactact ttcatagctg aacctgggtc atgagaaact tttacatcat
     8281 gtaatctaaa taaatcaatt tccttatgag taagccaaac tgtgtgagca gctaaaagat
     8341 ttggtcctaa tacacctagt ttatcaagat attcaactgt tccaactcca ttgttttgct
     8401 ttacaaatga aatttcatca gctatttcag caatatgcat atgtatgcct gtattgtatt
     8461 tatcagctaa ctctttagtt ctaacaagta attcttcagt tgtattaaaa atagttctta
     8521 atgcaaacca tattttaatt ctctcattag atgtgttatt aaacttatgg aataagtcta
     8581 attgagtgtt gatttcttca tcagtagttt tttgccatgc ctcaggaaga ccgtctcctg
     8641 tatccataac tgatttagct aaacatgctc taagtccagt tttatcaaca gcttcaacca
     8701 tagcatctac atattgacca ccagattcta aaaatgtagt gacaccagat tttatcaatt
     8761 caacacaaca tgcagtagat gaaatcaagg aatcttcata attgaagcta ctttcgtatg
     8821 gccaaattct ttctctaagc caagttaaaa gtacaacatc atctgcaaga ccacgtccta
     8881 attgttgaga taagtgaaca tgtgtattga ctagccctgg cattagtatt tttcctttag
     8941 catcatatat ctctgcatct ttatctacaa tattaatatc aaacttacca acctctttta
     9001 taatattatt ttcaataagt atgcttccat tttcatatac ctctctatct gcattcatag
     9061 taattagata tgcatttttt ataagtaatt gtgacataaa caccactcct tttctatata
     9121 tttttatatc tatgtattat gataataaat tttctataca aagttaatag caaaatcaat
     9181 ttacatataa agtgattttg ctatttataa atttaaaata aataagattt tattctatat
     9241 aatttaaaat attgacctag aattatttaa aatattaact tagaactaag tttacaactg
     9301 caagtaaacc tataatccaa agtgtaatac tgattttatc agtttcttta gctaccacat
     9361 gcattattat atatgaaata aatccaaagc ataaaccaat acttatacta taagttaaag
     9421 gcatcaataa aactgtaaca aaagcaggta ctgctatttt gctatcagca aaatcaatct
     9481 gttttatgct ttcaaacatg aacacaccaa ccattacaag tgcaggagct gcagcgtaag
     9541 taggaactat accaagtaat ggtgtaaata taagtgtagc taagaataat gctgctgtta
     9601 ctattgaagc aagtcctgtt cttgcacctg ctgcaatacc agaagcagat tctccaaatg
     9661 ttgtaacagt actagttcct aaaaatgaac ctattaaagt agaagaaaca tctgcatata
     9721 acattcttcc taaatctttt atttcaccat tctcatcttc aagacccatt tgtttacaac
     9781 aagtaataag aaatgctaat gaatcaaaca tgtctataaa catgaaagag aatataggtc
     9841 caattaatga gaatttcaat gcacctacaa tatctaattt aaaagcaact ggtgctatac
     9901 taggaggtgt tgagattatt gctgttggca actccacaaa tccaaatata acagatatta
     9961 ttgttgcaac tactatactt attaatattc cacctttaac ttttttatat tctagtattg
    10021 ccattaagat aagagcaaaa agagctatta aaactgttgg tgtaaaatct gcaagtgaaa
    10081 ctatagtatc tggattagct attataacac ccatattttt taaaccaaca aaagcaagga
    10141 aaagacctat acctgctgaa gatgctactg ccaattctct aggaattgcg tctgcaattg
    10201 tctttcgaag acctgttact gcgagtgtaa agaagacaca tccagataga aaaactaccc
    10261 caagacctgt ttgccatggg attcctgccc ctataaccaa agaatatgtg aaaaatgcat
    10321 ttaatcccat accaggagct agagcaaatg gtgcatttcc taaaatcgca aatataagtg
    10381 taccaatggc tgtagctagt atggtaactg ttataagggc ggctttatcc attccagcct
    10441 cagataatat tgctggattt acgaaaacta tgtatgccat tgttaagaat gtagtaactc
    10501 cacctattac ctctgtttta aggctagagc cacgttcgct aaatttaaac attttgtcta
    10561 aaaagcttaa tgatgaatta tttccacctg gttgtacatt gttagtgttt ggtaccattt
    10621 ttacctccaa aaaactttaa gatataaaca aaaaaatata tattcatata gatagaatag
    10681 aacatatatt caaagttgat attctattca tagaaaaccc atttgaggtg agtttgtaga
    10741 aactattaga ccgtattact aatattatat gaacatttt
//
LOCUS       DAEMAE010000125        10701 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEMAE010000125 DAEMAE010000000
VERSION     DAEMAE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.127"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1340
                     /locus_tag="KPH93_002469"
     CDS             <1..1340
                     /locus_tag="KPH93_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG1689276.1"
                     /translation="RYVIPIKSSYKNEVNGTILDTSSKGNTVFIEPISVSNLSTELTM
                     LKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVFAKAKYSQKIKGITPKIN
                     NNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAGGKTVTLKTVGLLTLMVQ
                     CGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIKNIAEIMSLSNNSTLVLF
                     DEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKKFATLHPEFENAGMMFDK
                     ETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVIDRNYNLDLVKRSKLQSN
                     IEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFNNVEVLFNDNFISVNIKR
                     LQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKTLKKIQKEIKENRK"
     gene            complement(1403..2026)
                     /gene="ytaF"
                     /locus_tag="KPH93_002470"
     CDS             complement(1403..2026)
                     /gene="ytaF"
                     /locus_tag="KPH93_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG1689277.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLFGCLLGNMFVKNINLNLSWLSGLILLVIGFMKIC"
     gene            2214..2858
                     /locus_tag="KPH93_002471"
     CDS             2214..2858
                     /locus_tag="KPH93_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689278.1"
                     /translation="MEQHSKVGNFGDLLKILLEEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNTDSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            3068..4126
                     /locus_tag="KPH93_002472"
     CDS             3068..4126
                     /locus_tag="KPH93_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG1689279.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HQEEISEFNGFVFENEFNAEEILTYYYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKEYINDHIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFRMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            4230..5414
                     /locus_tag="KPH93_002473"
     CDS             4230..5414
                     /locus_tag="KPH93_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1689280.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTSSGDLLGSVDIVSNNSIDNISLKTKLKMKF
                     AN"
     gene            5621..6334
                     /gene="rpsB"
                     /locus_tag="KPH93_002474"
     CDS             5621..6334
                     /gene="rpsB"
                     /locus_tag="KPH93_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG1689281.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            6413..7324
                     /locus_tag="KPH93_002475"
     CDS             6413..7324
                     /locus_tag="KPH93_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG1689282.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            7408..8112
                     /locus_tag="KPH93_002476"
     CDS             7408..8112
                     /locus_tag="KPH93_002476"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG1689283.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            8138..8695
                     /gene="frr"
                     /locus_tag="KPH93_002477"
     CDS             8138..8695
                     /gene="frr"
                     /locus_tag="KPH93_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG1689284.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            8698..8901
                     /locus_tag="KPH93_002478"
     CDS             8698..8901
                     /locus_tag="KPH93_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689285.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDKQNINYTIKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            8930..9664
                     /locus_tag="KPH93_002479"
     CDS             8930..9664
                     /locus_tag="KPH93_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG1689286.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            9677..10456
                     /locus_tag="KPH93_002480"
     CDS             9677..10456
                     /locus_tag="KPH93_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG1689287.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGVPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         4457 a         1100 c         1888 g         3256 t
ORIGIN      
        1 atagatatgt aataccaatt aaatcttctt ataaaaatga agttaatggt actatattag
       61 atacatcatc aaaaggaaat actgtattta ttgagcctat tagtgtttca aatttaagta
      121 cagagttaac tatgctaaaa gctgatgaaa ctattgaaga atacaaaata ttatcttatt
      181 taacagaact tatatttgag aagataagtc agataaaatt aaatatagaa atactttcag
      241 aatacgatat ggtatttgca aaagctaagt atagtcaaaa aataaaagga attactccaa
      301 aaataaataa taatggctat ataaaaataa ttaaaggtaa acatccactt ctaacaggag
      361 atgcagtacc tttagatttt gaaataggca aaaattatag aagtcttata ataacaggtc
      421 ctaatgcagg aggaaaaact gtaaccctta agactgttgg tttactaact ttaatggtac
      481 aatgtggatt tgatatttct gcaaaagaag gtagtgaatt tagtgtattt gaaaaagttt
      541 ttgtagatat aggagataat caaagtattg aaaatgcctt aagtactttt tcatcacata
      601 taaaaaatat tgcagagatt atgagtttat caaataattc tactcttgtt ctgtttgatg
      661 aaataggaag tggaactgaa cctaatgaag gagctggttt agcaatatca ttattagaag
      721 agttttacaa aatgggatgt ataactatag catcaactca ttatggagag ataaaaaagt
      781 ttgctacatt gcatccagaa tttgaaaatg ctggtatgat gtttgataaa gaaacattag
      841 aaccattgta taagttaact attggaaaat ctgaagatag taatgctctt tttatttcta
      901 aaaaaatggg aataaaaaat agagttttag atagggctaa agaatatgta attgatagaa
      961 actacaattt agatttagtt aaaagaagta agctacaatc taatattgaa gataaaaata
     1021 gctctgaagt cattttaaac tacacagatt ataatgtagg ggataaggta aaattactag
     1081 atgaagatga ttttggaatt gtatataagc ctatggataa gtttaataat gtagaagtat
     1141 tgtttaatga taattttatt tcagtaaata taaaaagatt acaacttagc ataaaagcat
     1201 cagaattgta tccggaaggg tatgatttag atgtattatt tactagtttt aaagaaagaa
     1261 aactagaaaa agatattcaa agaggttcaa aaaaaactct taaaaagatt cagaaagaaa
     1321 ttaaagagaa tagaaaataa atataaaaag cacaaaagaa agcaatctaa agagaaaatt
     1381 ttatctttag attgctttct tattaacata ttttcataaa tccaattact aacaagatta
     1441 aaccacttaa ccaagataag tttaaattta tgtttttaac aaacatattt cctagtaaac
     1501 aaccaaataa tactgcaatt atatttgata ttaaagaaaa tagaataact tgtatataat
     1561 tcacacctac aagaccacta ccaaaaccga tagctaatcc atctaaactt aaagcaagtg
     1621 caagtgtaaa tgcttcagca gatttaagca ctttagattt acttgtatct aagacatcag
     1681 gttcgatact tacattaaaa tttaatttaa ggtctaacat attaaaatta taattctttg
     1741 agtgtataga ttttttctta aaataagatt taaaaaaact ttcaattagt ctagcacttc
     1801 ctaataaaaa taagataaag aaacatatta acattgttat attttgaggt aaaaagtctc
     1861 taactattgt acctgcaaaa agcgatattg ctaaaatagc tgaacaaact acgctgataa
     1921 ttatatttga atataaggga actttaactt tgtttacgcc atatccaaag cttgtcacaa
     1981 aggcatctat acaaagagaa gtaactaata aaattgattc aatcacagta gattaacatc
     2041 ctttctctta aattttgtac ttatattagt tatagtattg taaaaatgga taaattgtta
     2101 cagataatcc aatagattgt tgataaaatt tcaacatcat acaacaccta aataacaaaa
     2161 aaatcatatt atatagtaat ataaaaccaa tcataataaa gcaggtgaaa ataatggaac
     2221 aacatagcaa ggttgggaat ttcggtgatt tgttgaagat tttattagaa gaaaaagaat
     2281 tatcaatggg agaactaagt aaaaaaagtg gaattgataa atctactata tctcgtatag
     2341 ctaataataa acaaaagcca aatataaatc atttagaaaa gatggcaata catttaaaca
     2401 ttaacctaga agaactattg aaagcttcag gatatgaatt tagaaacagc aataatcaaa
     2461 tatttaatac agatagtgat tttagtaact ttgatgatat tcttgggttt gcaaatttaa
     2521 tcaatgataa gaactttaat ggaaatattg aaaaagagtt aagtaagtgt aagttatatg
     2581 tccaaacaga tgaaggtaaa aaattacttt ttgataattt taataaaaaa atagattcta
     2641 ttgaaaaaca aggtcaattt acagatagat taagacaaat gtatgccgat ttttgtacag
     2701 atggacttac aataaagaaa tatttattga ttggaagtat gctactttac tttgttatat
     2761 caacagatgt tattccagac ttcgtgttcc caataggatt tatggatgat ttagtagcat
     2821 taaatatagt tacaaaatta cttaaaaatg ataagtagtt ttaaaattac atttaatatg
     2881 taaataaatg aaagtatata taaaatttat tattttttat aagaaataac ttaaaagtac
     2941 aataaacaga ttaaaaattt aaaactaaga atttaatata tattttgtaa ctatttaagt
     3001 atataaaata aaataatatc caaactaata caaaatagta ataattgttt tgtactggag
     3061 gatatttatg aattgtaaat ggatatctaa aatagatgag agaaaaaaat gccatagaga
     3121 agcagattct tctggatatt gtatctttca taaagaaaat aagtctgatg aagagataca
     3181 gttgatgatg gatactcttc atcaagaaga gataagtgaa tttaatggat ttgtttttga
     3241 aaatgaattt aatgcggaag aaatattaac ttactattat aaaatactgg atttttctga
     3301 atccatattt aaacaaaaag caaattttaa aaaatatatt tttaaaaaga atattatttt
     3361 taactataca gaatttagag ataaagtttt atttaatggt tgtgtattct tagaaaactg
     3421 tgattttaat agaaccatat ttagtaaaga atatataaat gaccatatat ttgaaaaagt
     3481 gaaatttaaa ggacctgatt tggtagttaa taaagtagaa aattttccac ggatggatgg
     3541 aataattttt agcatgtgta caaaatttgt gttaaagaat gttgaatatg gaaaaagtga
     3601 gtatgagcat ggaaaaataa attatagaat agctagaaat caagcaacaa aaattggaga
     3661 atatgagatg ataggatttt actattataa ggaaagaatt tatagtagta aaataatgaa
     3721 acgttctaat tatcctactt tcagtgatta tttagttgaa aaattttttg accaaatagc
     3781 acgttatacg acaggttatg gagaaaagcc atggaatata ttattagtga ttatagcaat
     3841 aataagtgtt tttgctttat tatacttatt tgtaggcata gaaagttcaa atagtacact
     3901 tgtagcttta gatataaaca atataggtga ttactcacta agtgagattt tcagaatgta
     3961 tatggattta tggtatttta gcatggccac atttagtaca gtaggatatg gagatatggt
     4021 tgctacaagt ttgattggga aagcattggc aggtatagaa gtattttttg gagttacaat
     4081 aggagcaatt tgggcatctg tgattattaa aagaatgata aggtaatatt tgtgtaaata
     4141 tggataggta ttaattacta gatataaatt ggactaaata gggaaaattt aaatataaat
     4201 atacttaata atccaagaag gtgaaaaatt tgaaaaatgc actaaaaaaa attatatcat
     4261 tagccatgat tctagttttt ataataccaa attattcata tgcagatggt aaatctgtag
     4321 acaagtattc aaaaacatct atacttatag accaagaaac aggtagagtt ttatatagta
     4381 aagaacctga tatgaaagta ccattagcta gtttaagtaa gatgatgact tttttacttg
     4441 caatagaagc tattgaaaat aaagaagtaa aagaaactga taaggtaaaa atagacaaat
     4501 ctattgcatc agtaaaaggg tcaagttata aacttaaaga tggtgaagaa gtgccactta
     4561 tagagcttat gagaggtctt atgattgtat caggaaatga tgcggcaatt gctatagcaa
     4621 aacatatttg taaaacgaaa gaagcatttg tagatagaat gaataaaaaa gcaaaagaaa
     4681 taggaatgag caatacacat tttctaaatc caaatggtct tccaatatac gatttaagca
     4741 accccaaaaa accagcaaaa gaaaatatat ctactgcaaa agatatagct attcttggaa
     4801 aatacatgtt tgaccattat gaaaaacaag taacagctat aactgatatg gagacatata
     4861 caaattcaga tagaagtttt gaaaaaagca atacaaatgc attattaaga attataccag
     4921 aagtagatgg tataaaaact ggatatactg gcaatgctgg atattgttta tcattttcta
     4981 tggttgtaaa taaaaatgat aaaaatgaga aaaaacgtag agtaataggg gttgtattag
     5041 gtactaatca taaaaataaa agaacctctg cctcattagc tctactaaaa tatggaaaag
     5101 aaaatttcaa tatgaaaaaa gtaattgata aagatagctt tattggtaag aaatatataa
     5161 aaggaatgaa agaactagag gttacattaa aagcaaaaga tgaactttat acaatattaa
     5221 aagatgatga aagtataaaa tcagaagtta agattaaaaa tatagagtac cctgttaaaa
     5281 aaggcgatac tatgggtatt ataaaatatt atactagttc aggtgactta cttggaagtg
     5341 tagatatagt aagtaataat agtatagata atatatcatt aaagacaaaa ttaaaaatga
     5401 aatttgcaaa ttaaattcaa aaaatggtat aaaatacaca aaaaaatata aaaatagaaa
     5461 atatggaaaa aatattgtta tttcttcacc tctgtgatat actactagag gtgaaaaaat
     5521 aaaataaatc acacacagca ttgggatcct taaaaggtgc ctattacatg gttttttagg
     5581 gagaagatca agctggaggt ataaaaccag gaggtaagat atgtcagtaa tatcaatgaa
     5641 acaattatta gaagcaggtg ttcactttgg tcatcaaaca agaagatgga accctaaaat
     5701 ggctaagtat atattcacag agagaaatgg aatatatata atcgacttac aaaaaacagt
     5761 taaaaaagtt gaagaagctt acaagttcac taaagaagta gctgaaacag gaaagccaat
     5821 cttattcgta ggaactaaaa aacaagctca agatgcgata aaagatgaag ctgaaagatg
     5881 tggaatgtac tttgttaatg aaagatggtt aggtggaatg ttaacaaacc acaaaactat
     5941 aaaaacaaga ataaataaat taagagaatt agaaaaaatg gaagaagaag gcgtatttaa
     6001 tgttcttcct aaaaaagaag ttataaaatt aagagctgaa aaagaaaaac tagaaaaata
     6061 cttaggtgga ataaaagaca tgcctgaatt accaggtgca atgttcgtag ttgacccaag
     6121 aaaagaaaac atagctattc aagaagctca tagattaggt ataccagtag ttggtatagt
     6181 tgatactaac tgtgacccag aacaattaga ctttgctata ccaggaaatg atgacgctat
     6241 aagagccgta aaattaataa ctggtgctat ggcaacagct gtaattgaag gtagacaagg
     6301 tgctgaagaa gaagtagctg aagaccaaga ataataattt gacaattaaa tggtaagggt
     6361 cgcccttacc atttataata tggaatagaa taagtaggag gaaacttaaa aaatggctaa
     6421 tataactgct caaatggtaa aagagttaag agaaagtaca ggcgcaggaa tgatggactg
     6481 caagaaagct ttacaagaag ctgaaggaaa catggaaaaa gcagtagatt tattaagaga
     6541 aaaaggatta tcaaaagctg ctaaaaaagc tggtagagtt gctgctgaag gtctagttgc
     6601 aatagaaatg aatgatgata atacagttgc ttctatggta gaagttaact cagagacaga
     6661 tttcgttgct aaaaacgaag acttcaaagt gttcgttaaa gatgctgctt gtatggcatt
     6721 agctactgat aaagaagata tagcttcttt attaggtgaa actcataaag agggaataac
     6781 tttacaagaa gttttaaata atagagttgc aaaaataggt gaaaaattag actttagaag
     6841 atttgctaaa gttgtaacta atggacaagt tgctggatac attcacggtg gtggaaaaat
     6901 aggtgttctt gttgaaatgg aaacagaagc tagagatgct aaagttttag aattaggtaa
     6961 agatgtagct atgcaagttg ctgctatgaa tcctaagtat gtttcaagag atgaagtaga
     7021 tgctgaatac atagcacatg aaacagaagt attaactcaa caagctttaa atgaaggaaa
     7081 accagctaat atagttgaaa aaatggttaa aggaagatta gaaaaagaat taaaagaggt
     7141 ttgtttatta gagcaaactt tcgttaaaaa tccagatata actgttaaac aattagttgc
     7201 tgatgttgct aaggcagtag gttctgatat aaaagttgtt aaagttgtga gattcgaagt
     7261 tggtgaaggt atacaaaaga gagaagaaaa ctttgcagaa gaagttgcta agcaacttaa
     7321 gtaataacca aagagagaac acgcaggtgt tctctttttt aaaaaataga aaaaatggat
     7381 atgaaggatt gaaataggag gtttctgatg gataaaccaa tgtacaaaag ggtattgtta
     7441 aaattaagtg gagaagcatt agcaggagaa agaggttttg gtataaataa tgatgtagtt
     7501 aatgatattg ctattgctat aaaaaaaata caagaaatag gtgttgaagt agctgtagta
     7561 gtaggtggag gaaacttctg gagaggtaga actagtgaag gaatggatag aactactgct
     7621 gactatatag gaatgttagc tacagttatg aatgcaatgg cgctacagga tgcactagaa
     7681 aatatagatg ttgctacaag agttcaaact gctatagaca tgagacaaat tgcagaacca
     7741 tatataagaa gaagagcagt tagacactta gaaaaggaaa gagttgttat atttggtgca
     7801 ggaacaggaa acccttactt tacaacagat acaactgctg ctttacgtgc tgctgagatg
     7861 gaagcagaag ttatattgtt agctaaaaat gttgatgcag tatatgacaa ggatccaaaa
     7921 gtacatgcag atgctaagaa atttacagaa ttaagttaca tggaagttat acagaaagaa
     7981 ttaaaagtaa tggattctac agctacatct ttatgtatgg ataataaaat tccaataaag
     8041 gtatttgagc ttacaacaga gaatataata agagcagtaa aaggcgaaaa tataggaact
     8101 actgtaaaat aatgaagaat gaaggaggag taaaattatg aaactggaaa tacataagca
     8161 attagaagag aaaatgaatg ggacaattga tgctttaaag tttgaatttg gaactataag
     8221 agcaggaaga gctaatgcac aaatgctaga caaaataaga gttgattatt atggaactcc
     8281 aactccaata aatcaaatag gtgctatatc tgtaccagaa ccaagaatat taatgatatc
     8341 tccttgggat aaatctgcta tgcatgaaat agaaaaagcg atagctaact cagacttagg
     8401 attaaatcca tcaaatgatg gtgaggttat aagactttct gtacctgctt taacagaaga
     8461 aagaagaaaa gaattagcta aaaaagctag taaagctgca gaagaattta aagttagaat
     8521 aagaaatgaa agaagagacg ccaatgagaa aattaaaaag atggaaaaag gcggagaatt
     8581 aactgaagat gaattaaaaa aagcacaaga tgaagttcaa aaaatgactg ataaatttat
     8641 aaaagaaata gatacattat tatctaaaaa agaaaaggac ataatggagg tataatattg
     8701 aaaaaatatt ataacctttt aggtcttcat gtagatgagg taaaacaata ttttgataag
     8761 caaaatataa attataccat aaaatctatt gaaggtaaaa aggataaaga aaaacttgta
     8821 gttcctagag ttataaaaat atctgagata ggtgatagtg tagaactgat aatcacatac
     8881 ttttctgact ccttagttta aggagtcaga acaatattgg aggcaaaata tgaataacaa
     8941 cattatatat gatatagact taaataacat acctactcat attgctatta taatggatgg
     9001 aaatggaaga tgggcaaaag ctagatttct tccaagaact gcaggacata aggctggagt
     9061 agaaactata agagatatag taaaagaatg ctcaaaatta ggtgttaagc acttaacatt
     9121 atatgcattt tcaacagaaa actggaaaag accgaaactt gaagttgaca cacttatgaa
     9181 cttattatct acatacttaa gaaatgaaat agctgaactt caccaaaaca atgtaaaagt
     9241 aactgctata ggagatataa gtgctttacc aaaaacttgt ataagagaat taaactcagc
     9301 taaggaactg acaaaaaaca atacaggagt taacttaaat ttagcattaa actacggaag
     9361 tagagcagat ataaaaaatg ctcttataga tatagtaaaa aattgtgaaa gtggtaaaat
     9421 agatataaac aatattgatg aagatattat aaaaaactat ttaagcacaa agtctatacc
     9481 agatccagac ttggtaataa gaacaagtgg tgaacaaaga ttgagtaact ttttactttg
     9541 ggaagttgct tattctgagt tttactttac tgatatacat tggcctgatt ttaaaaaaga
     9601 agaactacag aaggctatat acgtatatca aagaagggat agaagatttg gaggacttaa
     9661 gtaaggagat tattttatgc ttacaagaat tattgcttca ctagctctgg ttccattatt
     9721 tctatttgtt gtatatgggg gtgttcctct atatatagct gagacagcaa tagtttatat
     9781 tgcattgcat gagttttata aggcatttaa aataaaagat gtacatccaa tattcattat
     9841 aggatattta ttttctattt atttagctgt aaaaaatatt tttaatttac cattagaata
     9901 tacttatgca gtaattttta ttttattttt agcaagtata atttatatgt tgatgggcaa
     9961 gaacaatgtt atagatgttt cgattacgtt tttaggagtt ttttatatag gagtattttt
    10021 agattttata attataacta taaatggatt tgaaaaaggt agcatatatg tatggcttat
    10081 atttgtgata tcttttatga cagatatatt tgcgtatttt agtggttatt tacttggaaa
    10141 acataagcta attcctaaag ttagtcctaa aaagactata gaaggagcta ttggagggat
    10201 aataggaagt acactttgtt gtattttatt tggatattta tttggcattg atttgcttca
    10261 attagcgata ataggaagta taggaagtgt aatagctcaa ttaggagatt tatttgcatc
    10321 ttctataaag agatatgtag ggataaagga ttatggaaag ataatacctg gtcatggggg
    10381 tatattagat agatttgata gtgttatatt agttgcacct tttgtatata gtgcgattaa
    10441 attttttatt agataaagat tggttggatt taaaagagca atctataatt gctagtaatg
    10501 aaaatagcta tgtaactttt aaatgcagag atattctgca gttattagtt acatggctat
    10561 tttcattgtt caaaaattta gaagatattg gggaattgta taagaatatg tattacttta
    10621 taataagata ctatgtttgt agtaagtata ttttttatac tataactatc attaaaaaat
    10681 atttttttgt ttaataattt c
//
LOCUS       DAEMAE010000126        10529 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEMAE010000126 DAEMAE010000000
VERSION     DAEMAE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.128"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..242)
                     /locus_tag="KPH93_002481"
     CDS             complement(<1..242)
                     /locus_tag="KPH93_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689288.1"
                     /translation="MFKKENRYMTREIAENLPIEITILLWDLIDNLDIEKDYLQIFEI
                     NPIGLGVVEIVHRQEVPEYEASIYIQNDLIKYKLKI"
     gene            complement(383..844)
                     /locus_tag="KPH93_002482"
     CDS             complement(383..844)
                     /locus_tag="KPH93_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG1689289.1"
                     /translation="MDKKKIPAKRVDIVSLKLVKESSVLYETRKISNPYDAYRLVKNF
                     LVDSDREKFVVVCLDTKNQPVSIEIVSIGTVNSAMVHPREVFKVAVLSNASKIICFHN
                     HPSGNTNFSKEDEVITKRLQKCGEILGIELVDHIVVGDDDKYFSFKENFKI"
     gene            complement(908..1243)
                     /locus_tag="KPH93_002483"
     CDS             complement(908..1243)
                     /locus_tag="KPH93_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689290.1"
                     /translation="MINLFKKEDLNLLLEYPKEVVENVDNVINILDESYGDNRKITDN
                     GGYVCIIEDIKEIEYLKSHILNGLIEEFSDVIYESEVDIYNSTLYLLSSDYSITIISK
                     NEETEHLLK"
     gene            complement(1687..1833)
                     /locus_tag="KPH93_002484"
     CDS             complement(1687..1833)
                     /locus_tag="KPH93_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689291.1"
                     /translation="MTDKIIKPKNLFIYELKCDLIEDDERFVIKGKLDKEEINNLIFY
                     LQGV"
     gene            complement(1851..4832)
                     /locus_tag="KPH93_002485"
     CDS             complement(1851..4832)
                     /locus_tag="KPH93_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBG1689292.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVEDLFRFL
                     EITQKELLDEFKYNRGSNYKKAFIDLINSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKQDRNPKETLFGFNQRVFVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     MGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKKSKRAIFPRFHQLDAV
                     RKIVADLEVNKAGKNYLIQHSAGSGKTNTIAWTAHRLSSLHDEDNNAIFDSVIVVTDR
                     KVLDKQLQDAIYQLEHKNGLVVKIDDDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLEKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKIIDAENEKNEKSSIDKMN
                     DLIEKRTNLDHISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPVSVYYKVGKKIEDNPEFDKGEAKRAINKFISLNEHNIRQKVETIVDDFVDNR
                     SMWLEGRAKGMIVTASRLHAVKYKLAIDQYIKEKGYNMKSLVAFSDTVKDEDIEYTEV
                     GMNKEVALDINDSNLPSIFEKSDFRLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKHTFILDFQNSVEDIQEAYKPYFEMTNIDKVTEPNVVNDLWYQLHEY
                     GIYTDEEVNDFAVLFYKDKRTNTQDAKMNSLIDKAVERYCYLDEEDEKSADEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLRKLPKKDKERIDIDDILSLDYYKLQKL
                     GEDGKKGSDISLYNGEGELVGISETATSRVSEADEEYLEDIIQKINDVFGVGLTEEDR
                     IIIDQYEEMFKNDKSIMDIAKANSYEDLVSTFGKSYFKRGIVKTKNMNDRLVKEILTN
                     NNLQNYMIHYLAEKIYAEANR"
     gene            complement(4894..5796)
                     /locus_tag="KPH93_002486"
     CDS             complement(4894..5796)
                     /locus_tag="KPH93_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689293.1"
                     /translation="MKLRQNYYELEEFIHREIQDYVCKSAQEGFKLSIQSSYKLYKNL
                     HTNTIRADIQKERVNIVNAIPIYNTSQKNNKKYTPDIILYTDNNREIYCEISVYKSVN
                     TADYFEEWKVLGKTVIEIRKTEIMDMHLDGYRLSKDLIISYLYDPILDKARKQKISES
                     QKIIDKSNNIISKHKNLNKENNVKENNSKEKIREKEYKEFDDKVNYDIKKHLNNLNEN
                     NILIKEYVDKRTDKQKRSDKRNGREPKKELVYFDVKSNKEARIIYAVIMKNSKRAYRS
                     IPAIIGKYLRDDGYKVGVAGKKRK"
     gene            complement(5872..6192)
                     /locus_tag="KPH93_002487"
     CDS             complement(5872..6192)
                     /locus_tag="KPH93_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689294.1"
                     /translation="MLYVGDYIDMKTLNIRYKDGFKIEEDTRLYPINSDINDKIGVLV
                     EHRKLNATNRYLRYGIQKISGKCYFFDVRNSVISKTKESAYNTFICPYCRERMFIVSA
                     FERQ"
     gene            complement(6226..6678)
                     /locus_tag="KPH93_002488"
     CDS             complement(6226..6678)
                     /locus_tag="KPH93_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689295.1"
                     /translation="MKLSRKNITMQVLSKFIENNLEKIENVETKANQIVFRTIDGDKK
                     IKIKTSKNYSEGEDPNTYLWTSFSSEELEEDYDYYTIICNEDIEKAVVFTKEELKQHY
                     VLETKKKKNGEIDFELYPHFIGRRCIDARVHLYKEPIDITKYVNNYIF"
     gene            complement(6710..8077)
                     /locus_tag="KPH93_002489"
     CDS             complement(6710..8077)
                     /locus_tag="KPH93_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG1689296.1"
                     /translation="MRYRYRDDEEMKDSGVEWIGKIPKDWEVKRIKHLFELKKDKSDE
                     ENPTVLSLTQKGLKIRDVSNNEGQLASTYVGYTKIEKNDFILNPMDLISGYTDKAEIE
                     GVISPAYTTFRSKNKVNINHDYYKRYFQMHYHHNFLFPWGEGVSFEHRWTLKNEVFLN
                     LPVITNRLEEQEKIANFLDEKTSQFEFIISKKEELIKKLEEAKKSLISEVVTGKVKVV
                     KTDDGYKLVKRSSEEMKDSGIEWLGEIPKDWEVKNFKYMFTLNKGLSITKADLKESGI
                     PCVNYGEIHSKYRFELKPSKHKLKYVDESYLKSNSISLLKYGDFVFCDTSEDIEGCGN
                     FTYLNENNKVFAGYHTIIARTLEQVNYRYMSYLFDSNDWRIQIRTKVSGVKVFSITQS
                     ILKGTKVILPDLLEQRNIAQYLDSKCKGIDSIIDKTKLQIDKLKEAKQSLISEAVTGK
                     IEILD"
     gene            complement(8077..10104)
                     /locus_tag="KPH93_002490"
     CDS             complement(8077..10104)
                     /locus_tag="KPH93_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG1689297.1"
                     /translation="MSIDNNFNHIAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIVKSVYKEYDFLPEEEKDELVIVDLKENHNIDLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEIAQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNEMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEMLFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKVNPSAIVNVYGQELLDESFAICQADMIMKGQNPDNIRLGNT
                     LTQDRFSGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEDKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEEIKEI
                     YERFEESENSKIFDNEGFGYRKVTIERPLKLSFRVNEEAIENVKNTTQFINLSVSKKK
                     DEEVKVKEEAEGRVKQDKLLKLLESFDSEFEYMKRNKFIKDLKSKSKLYDVALSAGLI
                     KAIVNAIGVRNEDAVVCKDAKGNIESDSSLKDTESIALKEDVYEYFEKEVKPHVEDAY
                     IDESSIDNIGYEIPFTRYFYKYEKLKSFDDIMKEVESLESEIALEIRKVLG"
BASE COUNT         3113 a         1843 c          969 g         4604 t
ORIGIN      
        1 tatattttta atttgtattt aattaagtca ttctgtatgt atatactagc ttcatattca
       61 ggtacttctt gtctatgaac tatctcaact actcctaatc ctataggatt tatttcaaat
      121 atttgtagat aatctttttc tatatctaag ttgtctatca aatcccatag taatatagtt
      181 atttcaattg gtagattttc agctatttcc ctcgtcatat atctattttc ttttttaaac
      241 attttacaac tcctatttag tttaattatt tatatgtata ttattttttc aagggctttt
      301 cgggcttaac ttaaaactaa tttatttcaa ttagtttaga acatagaaaa agtcttagcc
      361 taatttcttt aagctaagac tctcatatct taaaattctc tttaaagcta aaatacttat
      421 catcgtctcc aacaacaata tgatctacta attcaatacc taatatttca ccacacttct
      481 gtagcctttt agttataact tcatcttctt tactaaagtt agtattacct gatggatgat
      541 tatgaaagca tattatttta gatgcattac ttaatactgc tactttaaat acttctcttg
      601 gatgtaccat cgctgaattt acagttccta tgcttacaat ttctatacta actggttgat
      661 tttttgtatc taaacatact actacaaact tttctctatc actatctact aaaaagttct
      721 ttactaatct ataagcatca taaggattgc ttatttttct agtttcatat agaacactac
      781 tttcttttac tagtttaagt gatactatat caactctttt tgcaggtatt tttttcttat
      841 ccatgattat ttcctccttt gtttctcaaa attaaaataa gcatatacac tttatcaatg
      901 tatatgctta tttaagtaag tgttcagttt cttcattttt agatattata gtaatgctat
      961 agtcactaga tagtaaatat agagttgaat tatagatatc aacctcactt tcatatatta
     1021 catcactaaa ctcttcaatt aatccattta atatatgtga ttttaaatat tctatttctt
     1081 taatatcctc aattatacac acatatccac cattatctgt tatttttctg ttatctccgt
     1141 atgattcatc aagaatattt ataacgttat ccacattttc aactacttcc tttggatatt
     1201 ctaataataa gtttaaatct tctttcttaa ataagttaat cattttaatt tcctccttat
     1261 aattttttga actaaaaaaa gagatgttag tatttctacc aacatctcaa tgtgttattt
     1321 tttgtatgtt cctctttagt atgatatcta tttaatgtat atttcaaaat tataatatat
     1381 atttataatt ataattatta cattttacta gattccttat ttattttgaa gttttacata
     1441 ctcttcttca aatacagtcg aattatcttg tacaatctct aactttatac cttctagcat
     1501 atcatataac ccatccacat ataggtttga tctattttgt atatattttc atctatatta
     1561 tctgttaatt ctatcgtttg atctattgat atatcttcaa tattttgtac ttcctcaaat
     1621 ccatatagtc tagttaatat attacttata tcgtatggta ttaatatgga attatctaat
     1681 atatttctaa actccttgta aataaaatat taaattattt atttcttcct tatctaattt
     1741 acccttaatt acaaatcttt catcatcttc aattaagtca cactttaatt catatatgaa
     1801 taaattttta ggttttatta ttttatctgt catattgtac tcctttttat ttatctattt
     1861 gcttctgcat atattttttc agccaaataa tgaatcatat aattttgaag attattatta
     1921 gttaatattt ctttaaccaa tctatcattc atattcttag tcttaactat acctctctta
     1981 aaataactct ttccaaaagt actaaccaaa tcttcataac tgttagcttt agctatatcc
     2041 ataatactct tatcattttt aaacatctct tcatattgat ctattataat cctatcttcc
     2101 tctgtaagtc ctactccaaa gacatcattt attttttgga ttatatcttc taaatattct
     2161 tcatcagctt cacttacacg agaagtagca gtttcagata taccaactaa ttcaccttca
     2221 ccattatata atgatatatc actacctttt ttaccatctt cacctaactt ttgtaactta
     2281 taataatcta aacttaaaat atcatctata tctattcttt ctttatcctt tttaggtagc
     2341 ttccttaaaa gtgctactaa gtaaacctgt aaccttaaaa gatttaagtt ctttataggg
     2401 taaatttgga ttaagaaatt ataaaaagta atatactttt gacatttttt cttaaactca
     2461 tctgctgact tttcatcttc ttcatctaaa taacaatatc tttcaactgc cttatcaatt
     2521 aaactattca tcttagcatc ttgtgtattt gttcttttat ccttatagaa taatactgca
     2581 aagtcattta cttcctcatc agtatatata ccatattcat gtaactgata ccaaagatca
     2641 tttactacat taggctctgt aactttatct atgtttgtca tttcaaagta tggtttatat
     2701 gcttcttgaa tatcctctac actattttgg aaatccaaga taaatgtatg cttattagga
     2761 tatgttctat taagccttga taaagtctga acagttttta caccatctaa cttcttatca
     2821 acatacatag cacataactt tggttggtca aaaccagtct gatacttttc tgcaactata
     2881 agtaatctaa agtcactttt ttcaaaaata cttggtaaat ttgaatcatt aatatctagt
     2941 gctacttcct tattcatacc aacttctgta tattcaatat cctcatcttt tacagtatct
     3001 gaaaaagcaa ctaaggattt catattgtac cctttttctt ttatatattg atctattgct
     3061 aatttatact taacagcatg taatctactt gctgtaacta tcattccctt agctctgcct
     3121 tctaaccaca tagacctatt atccacaaaa tcgtctacta tagtttcaac tttctgacgt
     3181 atgttgtgtt catttaaact aataaatttg tttatagctc ttttagcttc tcctttatca
     3241 aattcgggat tatcctctat ttttttacct accttataat atacactaac tggcataaag
     3301 ttctttaata cgtctaatat gaatccttct tctatagctt gcttcataga atataaatga
     3361 aactcatgag gtagtccatc ttctcctatt ctaccaaata cttgcatagt cttattctta
     3421 ggagtagcag taaatgcaaa gaaacttata tgatctaaat tagttctctt ttctataagg
     3481 tcattcatct tgtctatact agatttttca ttcttttcat tttcagcatc aattatctta
     3541 gcttcttcta atgtttttcc tgctaaagtt tcttttaatg cattcatatt ctcaccagat
     3601 gtagaagaat gagcttcatc tattattact gcataacgtc ttttttcaag ttgttctatc
     3661 ttaggtaatg catattggaa cttttgtata gttgtaataa taatctttgc tctgtttaca
     3721 atagcatcag ctaaatctga tgaattttta tcatcatcta tcttaacaac taatccgttt
     3781 ttgtgttcta attggtatat agcatcttga agttgcttat caagtacctt tctatctgta
     3841 actactatta cagaatcaaa tatagcattg ttatcttcat catgaaggct cgataatcta
     3901 tgagcagtcc atgctatagt attagtcttt ccactacctg cactatgctg gataagataa
     3961 tttttacctg ctttatttac ttctaaatcc gctactatct ttcttactgc atctagctga
     4021 tgaaatctag gaaatatagc tcttttactt ttcttatcat cttcttggat aaagtaaaat
     4081 ctctttataa tatcaagtaa actatcttta gtaagtattt cttcccaaag ataagaagtt
     4141 ttttcttttc cttctactac aggatttccc ttacccatat tgttaccttt attaaaaggt
     4201 aagaagaatg ttttatctcc ttttaactct gttgtcatat atacttcgtc agtatctact
     4261 gcaaaatata caaatactct ttgattaaat ccaaataaag tttcttttgg atttctatcc
     4321 tgtttgaatt gttttatagc attatacaca ttttgattag tgaattggtt tttaagttcc
     4381 attactataa caggaaatcc gttcaaagat aatactatat ctaaagtatt gttgtgcttt
     4441 gaactatatt ttaattgtct tgttattttg aatatatttt gcttataatt ttcgtaagct
     4501 gttttgttag tagttagatt tggtttgttg tagaataatt ggaattttcc gttcatcatt
     4561 atatcttcta caccatttct aagtacactt ataagccctc ttcttttgat gtttgagttg
     4621 attaaatcta taaaggcttt tttgtaattg cttcccctgt tgtatttgaa ctcatctagt
     4681 aattcttttt gagtaatttc taaaaatcta aataaatctt ctacgaataa gcaattttca
     4741 agatcaaatt cttgtgaatg accttgttcg tatccaattg aaactaaatg attttcaata
     4801 gactcttcta aatgtttctc ttttggtaac attgctattc ccccatttct atattgttaa
     4861 tctatatatt ataaagctta tattaatcat taactactta cgttttttac cagccacacc
     4921 aactttatag ccatcatctc ttaaatattt tcctattatt gcaggtatac ttcgataagc
     4981 tcttttgcta tttttcataa ttactgcata tataattctt gcttctttat ttgatttaac
     5041 atcaaaatat actaattctt ttttaggttc acgtccatta cgcttatcgc tccttttctg
     5101 cttatctgtc cttttatcta catattcttt gatcaaaata ttattttcat ttaaattatt
     5161 taagtgtttt tttatatcat aattaacttt atcatcaaat tctttatatt ctttttcacg
     5221 tattttttcc tttgaattat tttcttttac attattttct ttatttaaat ttttatgttt
     5281 agatattata ttatttgact tatctataat cttttgagat tcacttattt tttgctttct
     5341 agctttatct aaaatagggt catataaata agatattatt aaatccttag aaagtctata
     5401 tccatctaaa tgcatatcca taatttcagt tttccttatt tctattactg ttttacctaa
     5461 aactttccat tcctcaaaat aatctgctgt atttacagat ttataaactg atatctcaca
     5521 atatatttct ctattgttat cagtgtaaag tattatatct ggtgtatatt ttttattatt
     5581 tttttgactt gtattataaa ttggaatagc atttacaata ttaactcttt ctttttgtat
     5641 atctgctctt atagtattgg tatgtagatt tttatataat ttatatgaac tctgtatact
     5701 taatttaaag ccttcttgtg cacttttaca tacataatct tgtatctctc tatgtataaa
     5761 ttcttctaat tcataataat tctgtcttag tttcatactt ttagagtctg ttctaaatat
     5821 acattcttta tttgtctttt tataatgttt caagtgaatt ggtactatgc ttcattgtct
     5881 ttcaaatgct gatactataa acattctttc tctgcaataa ggacatataa aagtattata
     5941 tgcactctcc tttgtttttg atattacact atttcttaca tcaaaaaaat aacacttccc
     6001 cgaaattttt tgtataccat atcttaaata cctattagta gcatttagtt ttctatgttc
     6061 tacaagaact ccaattttat catttatatc actatttata ggataaagtc tcgtatcttc
     6121 ttctatttta aatccatctt tgtatcttat atttaatgtt ttcatatcaa tataatctcc
     6181 tacataaagc aatgtaaaat ataattttat tgattatata tattactaaa atatatagtt
     6241 atttacatac ttagttatat ctattggttc tttatataaa tgaactctag catctataca
     6301 tcttcttcct ataaaatgag gatataattc aaaatcaatt tctccatttt tctttttctt
     6361 agtttctagt acatagtgtt gttttaattc ttcttttgta aataccacag ctttttctat
     6421 atcttcattg catattatag tataataatc ataatcttct tctaattctt cactagaaaa
     6481 acttgtccat aaatatgtat taggatcttc tccttcgcta taatttttac ttgtttttat
     6541 ctttattttt ttgtcaccat ctatagttct aaaaactatt tgatttgctt ttgtttctac
     6601 gttttctatt ttttctaagt tattttctat aaattttgat aaaacttgca ttgttatatt
     6661 ttttctactt aatttcatat ttttcacctc tgttcaaata tactaagagt tagtctagta
     6721 tttctatctt tcctgtcact gcttcactta ttaaactttg tttagcttct tttagtttat
     6781 ctatttgtaa tttagtttta tctataatag aatctatccc tttacattta ctatctaaat
     6841 actgagctat attcctttgt tctaataaat ctggtagtat tacttttgta ccctttaata
     6901 ttgattgagt tatactaaaa actttaaccc ctgatacctt tgtgcgtatt tgtattctcc
     6961 aatcatttga atcaaataga tacgacatat atctatagtt tacttgttct aaagttctag
     7021 ctattatagt atggtatcca gcaaacacct tattattttc atttaaatat gtaaaattac
     7081 cacacccttc tatgtcttct gatgtatcac aaaaaacaaa atctccgtac tttaataatg
     7141 aaatagaatt cgattttaaa tatgattcat ctacgtactt taatttatgt ttactaggct
     7201 taagctcaaa cctatatttt gagtgtatct ctccataatt tacacacggt attccacttt
     7261 cttttaagtc tgcctttgtt attgataaac ctttatttaa agtaaacata tacttaaagt
     7321 tcttcacttc ccaatcctta ggtatttcac ctaaccattc aattccacta tccttcattt
     7381 cttcactact tctcttcaca agcttatatc catcatcagt cttaacaacc ttcaccttac
     7441 cagtaacaac ctcacttata aggctctttt tagcttcttc taactttttt attaattctt
     7501 ctttcttaga tataataaat tcaaactgtg aagtcttttc atcaaggaaa ttagctattt
     7561 tttcttgttc ttctaaccta tttgttatta ctggaagatt taaaaatact tcatttttta
     7621 atgtccatct atgttcgaat gataccccct ctccccacgg gaataagaaa ttatgatgat
     7681 aatgcatttg aaaatatctt ttataataat cgtgattaat atttacttta tttttacttc
     7741 taaaggtagt atatgccgga ctaattacac cttcaatttc tgctttatca gtatatcctg
     7801 atattaaatc cattggatta agtataaaat catttttttc aatcttagtg tatcctacat
     7861 atgttgatgc taattgtccc tcattattac ttacatctct tatttttaat cctttttgag
     7921 ttaatgataa taccgttgga ttttcttcat cagatttatc tttctttaat tcaaataaat
     7981 gttttattct ttttacttcc caatccttag gaatcttacc tatccactca actccactat
     8041 ctttcatttc ctcatcatct ctatatctat atctcattac cctagcacct tccttatctc
     8101 cagtgctatc tcactttcta aactttcaac ttccttcata atatcatcaa agctttttaa
     8161 cttttcatac ttataaaaat atctagtaaa aggtatctca tatcctatat tatctatact
     8221 actttcatct atataagcat cttcaacatg aggttttacc tctttttcaa aatactcata
     8281 cacatcttct ttaagtgcta tactttcagt atcttttaaa ctactatcac tctctatatt
     8341 ccctttagca tctttgcaaa ctactgcgtc ttcatttctt actcctatag cattaactat
     8401 agctttaatt agtcctgctg ataaagctac atcatataac ttagacttag attttaaatc
     8461 tttaataaac ttattcctct tcatatactc aaattcacta tcaaaacttt ctaaaagttt
     8521 taataactta tcttgtttta ctcttccttc tgcttcttcc ttaactttaa cttcttcatc
     8581 ttttttctta gaaactgaaa gatttataaa ttgagtagta tttttaacat tttctatagc
     8641 ttcttcattt actctaaagc tcagttttaa aggtctttct attgttactt ttctatatcc
     8701 aaagccttca ttgtcaaata ttttactatt ttcactttct tcaaatcttt catatatttc
     8761 ttttatttct tctatttgtt caagacttat ttcttttctt ttatttccta aactttttct
     8821 cattaattga taatattcac tagcatttac aagttgtatc ttaccttttc ttttatcctc
     8881 ttttttatta gtaagtaccc atatatatgt agctattcct gtgttataga acatatcagt
     8941 aggtagtgct attataccct ctagtaaatc attttctatt atccattttc taatgttaga
     9001 ttcaccactt cctgcatctc ctgtaaataa tggtgatcca ttgaatatta tagctattct
     9061 actaccttct tcatcatcat acattttgct tatcatattt tgtaagaata gtaacgaccc
     9121 atcacttact cttggtgttc ctgccccaaa tcttccatca aatcctaagt cagcttcttc
     9181 ttttactttc ttctcttcat ctttccatgt gacaccaaat ggtggatttg atattaaaaa
     9241 tcttatcttt tctccactaa atctatcttg cgtaagagta tttcctagtc ttatattatc
     9301 tggattttgt cctttcatta tcatatctgc ctgacatatt gcaaatgatt catctagtaa
     9361 ttcttgtcca tatacattta ctatggcact tggatttact ttttccacat agtcttcagc
     9421 tattgaaagc atcccaccag taccacagca aaaatctgct atagatatta cttttccttc
     9481 ttctgtaagg aagttttctt tgcccataaa taacatttcc atacaaagtc ttataacttc
     9541 tcttggagta tattgttctc ctgctgctga gttttctgta aatcttctta gcatttcttc
     9601 gtatatgtat cccatttcgt tgtttgatac agagtttata tgtaggtcta cttcatacat
     9661 tttgctaagg actgcatata gcttgtcttt tttatctaat tgagctattt catctttgaa
     9721 tttaaatttc cctattattt cttttacatt gtttgagaat ccatttaggt attcttcaaa
     9781 gttcgatttt atattttcac tatcatctaa cagttttttc catgtaaaat cactcacatt
     9841 gtagaactgt aagtctatat tatggttttc ttttagatcc actattacaa gttcgtcttt
     9901 ttcttcttct ggtaagaaat catattcttt gtatactgat ttcactattt ctctatcata
     9961 atcatctaat agacaatcaa atcttcttat tactattagt ggtaacatta cttttctgta
    10021 gtcttcttct ttgtatgtac cccttaaact ttctgctata ttccataaaa aagcaatatg
    10081 attaaaatta ttatctatac tcacttatac tatctccttt taattaaatt ttaaattagt
    10141 ttatattata attatatcat ttcatggcac aaaaaaaaca tattgacaaa actatcaata
    10201 tgtttttcgt ggatttcaat ggtggagacg aggggatgct acattcttat ttttacatct
    10261 tcatgtctca tcactttttg cgttatatca acacttataa tttttatatt tcatgtatat
    10321 ttttataagt tcatgtatgt tattaaatta gttgtgtgct aaatgtgtgc taaattcata
    10381 tattattcat taataaagtg gtatcgtcca aaacgatacc taataaatat aatatcttat
    10441 aaataataag cctatcatta tactctattt atttatgtag aatcgttcga caaattactg
    10501 atataagttc ttaaatatgc tataaatta
//
LOCUS       DAEMAE010000127        10444 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEMAE010000127 DAEMAE010000000
VERSION     DAEMAE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.129"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(30..728)
                     /gene="yunB"
                     /locus_tag="KPH93_002491"
     CDS             complement(30..728)
                     /gene="yunB"
                     /locus_tag="KPH93_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG1689298.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            942..3635
                     /locus_tag="KPH93_002492"
     CDS             942..3635
                     /locus_tag="KPH93_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG1689299.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNTPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            3935..4333
                     /locus_tag="KPH93_002493"
     CDS             3935..4333
                     /locus_tag="KPH93_002493"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG1689300.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            5244..5810
                     /locus_tag="KPH93_002494"
     CDS             5244..5810
                     /locus_tag="KPH93_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689301.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSINARNLAKTLNCSTKPL
                     FRIYTNMDEIKKDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAEWNRITIENAKKVTGLSIEKSEILFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            5828..6553
                     /locus_tag="KPH93_002495"
     CDS             5828..6553
                     /locus_tag="KPH93_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1689302.1"
                     /translation="MLESKIRTPEEIKRYMLDLKEWLAQERSKPVEEMSDFFSKRIDI
                     YDNIHLGNWAEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKRKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHEKNGTMIIKAEK"
     gene            complement(6926..7861)
                     /locus_tag="KPH93_002496"
     CDS             complement(6926..7861)
                     /locus_tag="KPH93_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG1689303.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNHLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(7905..9077)
                     /locus_tag="KPH93_002497"
     CDS             complement(7905..9077)
                     /locus_tag="KPH93_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1689304.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIVLKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(9064..9528)
                     /locus_tag="KPH93_002498"
     CDS             complement(9064..9528)
                     /locus_tag="KPH93_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689305.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(9543..10385)
                     /locus_tag="KPH93_002499"
     CDS             complement(9543..10385)
                     /locus_tag="KPH93_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG1689306.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKKRRT
                     SNKL"
BASE COUNT         3540 a         1781 c         1627 g         3496 t
ORIGIN      
        1 gaatttattt aaaaatatac aactttatat tacttgttgg tattctttcc agtgttaggt
       61 aaaacatttc ctagattttt ttctggaata tttacataac tttctggaac atcacctact
      121 attatagttt cacaaatagg tatttgagct ttaatttgtt ttgttgaagt tataagtggt
      181 attactactt taacttgtgt ttgagcttct aaatatattc tatgtctagt ttgatttata
      241 cctgaagact caaattccgt tttaaagttt actgatactg ttcctatagg ttcaatagac
      301 acttcaagtt taggaccgta tttagctaat ataggacttc ctaatgctgt tcctataggt
      361 atgtaagaac ttgctgtctt atcctttttt aattcatctt gtatttctaa agctattgca
      421 gatgcaattt cattcatcat tatagtgttt gcttgtatca tagttatttt gccattatta
      481 tcaagctgta tatccattaa atcttcataa tttatttttc cttctaccat ctcagcaaca
      541 gctttattta tcgacctatt tgctagttca agagctttag tctctgctaa aactgttatt
      601 gtcggtctta aggttttatc tatatatata aaactgccta taaatacaga caagaaaaga
      661 attactaaaa aaatagctat gatttttcta aattcatttt ttgtttttct atctactatt
      721 ctattcaaaa ttatcccctc ctatttattt atatgtagcc ttggcataat ttttataata
      781 tttgtcaaaa ataaataaaa taaaaatata gactttttaa tatattatgt ttatacatat
      841 acaatgtaca gaatattata gcaaattcat tttttctatt aattacaaaa ctactcctat
      901 ttgtgttata atattgcatt aagtaaggag gttaaaatct tatgaataac gataatgata
      961 aaaatggaaa taaaatcagg agaaaaaaag ttagctcatc tggtaataca aataaaccaa
     1021 ttaatagaac ttcagctaac aagactcgtt caacaaaaaa caagaaaaaa tctaaaaaaa
     1081 gcgataaatt taaaaaacta agagtatttg ggatagtatt tttagtttta ctagttgttg
     1141 gtacagcagg tactgctggt ctagtatttg cgtcactaag agatgtaact ccagttacag
     1201 aagctgtttt agataaacag acgaaccaaa caactactat taaatacgct aatggaaaaa
     1261 cattatctac agctccaagt gtaaataaaa agacacccgt tccattagat aagatttccc
     1321 cttatcttca acatgctgta attgcaatag aagatgagcg tttttatgag cataagggtg
     1381 tcgatattaa aggtttattt aggtcagtat taaagaccct tacaggtact aagcaaggtg
     1441 gtagtactat tcctatgcag gtatccaaaa tgctgttaac aactgaacag caaactatac
     1501 ctcgtaaaat aaaggatatt tactatgcac atgagatgag taaaacagtt agtaaagata
     1561 aaatactaga aacttattta aataacttct tcgttggtag aggtctagca ggtgctgagg
     1621 ctggtgcacg tggttatttt gataagtctg cagctgacct cactctagca gagtcagcac
     1681 ttcttgctgg ttcaactaaa aatccatcaa gattttcagc ctataaaact tctaagttag
     1741 agggaaatga aactaaggag gatttagaaa ataaattatt attttttgtt aacacaactg
     1801 atgatgattt agatgaccct actcaagtgg actttgatat gattgaaaaa ataaaatctt
     1861 gggagcttat atctaatgat acttatagac aattaaaagc aggtacatta gttgttagaa
     1921 aggctgtaag taaccctgaa gctaaaaaaa gacaagaaat tgttttaaag aaaatgttag
     1981 aattgaaata tataaaacaa agtgaatatg atgaagcaat caaagctaaa atagaaataa
     2041 aattgcctca atcatcagat aaagtttctt catctgtaga agaccttatc gaaagtgaag
     2101 ttataaatgc tttaatggaa caaggacata caaatgatga agctcaaaac ttattctaca
     2161 atggtggtct aattgtaaac acaactatag accctaaaat gcaagatgct ttagaagaag
     2221 aatttgatag aaatagcaat ttcccaggac atatggtcgg ccctgatgga gttagccaac
     2281 cacaagctgc aatggtaatc ttagattata aaaatggtga aataagagca ctagctggag
     2341 gaagaaatat aagtggtaga aaaactttga atcgtgctac taaccctcat cagccaggtt
     2401 cttctataaa acctttagca atatatacac ctgctattga taccctaaaa ataacacaat
     2461 caactgctct aagtgattct cgaggaggat ataagtttga agaaaataat aaatggaatc
     2521 caagaactac tactgctggt catggttcta tgagtttacg taaagcactt gctaaatctt
     2581 caaacacgat agctgtcaaa actgctgaaa tgcttggaga ctcttatgat gaatgtgtag
     2641 atataatgat ggattactta aagaactttg gtataacaac tttaaaaaat aatcactcag
     2701 gttcatctga agcaagtgat agaaaattcc catcattgac actaggtggt atggcgaatg
     2761 gtattactcc acttcaaatg gctgctgctt atggtacatt ggcaaatcaa ggtgtttatg
     2821 tagaaccttc tatttttact actataacta cctttgatgg tcaattatta gttaaaaaca
     2881 ctcctgaaga acataaagtt gtagaccctg aagtagctta tgttgttaca gatatgctag
     2941 aaagcgtaat tactgaaggt actggtggtg ttgcaacact tccaaaggga atgcctgtag
     3001 caggtaaaac aggtactaca aatagtgcat atgatgcttg gtttgtcggt tacactcctt
     3061 actatgttgg tgcaacttat attggagatg atgctggtag aaaagatgat tctggtaaca
     3121 ctataaaacg tagagaagta ccacatggaa gtactagtac tgcaaaacta tgggaaaaaa
     3181 ttatggaaaa aatacatgct aatttaacgg ttactgaatt tgaagttcct aaaaatgtat
     3241 attttacaaa aattaattta gaggatggag gaaagcaatc ttctggctct aaagcagcat
     3301 ttattgaagg tactgctcct actaaagtaa gttctcaacc atcatctgaa gatacaaaaa
     3361 aaccagacaa taaccaacca gaagaaaata ataataacaa taataacaca ggtgataatg
     3421 gtggcggttc tacacctcct gataatggcg gaaacaatgg tggtggctct actactcctc
     3481 ctgataatgg cggaaataat ggtggtggct ctgctactcc tcctgataat ggcggaaaca
     3541 atggtggtgg ctctactact cctcctgata atggtggaaa taatggtggt ggctctacta
     3601 ctcctcctga tggtggtgga actccagcaa cttaataaaa aatagtaaat atctggcagc
     3661 gataaaattt ttttttctag ccagaaaagc ccatgaaatg agcatttctg ctgtgttcgc
     3721 tacaaccctg aaacgggtaa attggtgctc taatggtgcc caagtgaaca aacagggtta
     3781 tgaacagcag tatgaagtaa tgaaacgaag taaaccgttt gacattttca cgcacgaata
     3841 ttagaatgaa aaaagcgagt aatgtgttgt gtgccttagg cggaaaaggc gtataaaaga
     3901 ttatttatat ggatacaaca ggaggatcaa cctgatgaaa tggaaggaac tttataataa
     3961 ggctagggga aaattaaatc cacgcacaat ttcacctttt atcgatgctg gtggggttgc
     4021 cgcagcaatc cttaccgaca gcggaaatgt ttatactggt gtgtgtattg atacagcctg
     4081 tacattggga atgtgtgcag aaagaaatgc tatcgccaat atgataacaa atggcgaaag
     4141 taaaattact aaattagttt gtgtgatgag caatgggagt gttggctcac catgtggtgc
     4201 ttgtcgtgaa tatttaatgc aacttgataa ggatagtgct tgtattgaga ttttagttaa
     4261 tgtcgaaaca gaaaaaactg ttaaattgag tgagcttttg ccggattggt ggggttccga
     4321 caggtttatt taactttatc aaatgagtat tttgtttcat gcaccttatt ggtataatac
     4381 gtacttccct tttatccgtt ctcaatagtg gcaagcagta cttcatggta cacacaaaaa
     4441 tattttacag gagtgtatat aatcttgcca tgattgttat gattgttttc ggagattcca
     4501 gtttgtcagg agggtaggac ctatctgtcc gttgtacgcc ctgcttcgtg ggcatggttc
     4561 ccacggggca taataaagag cgccccagat ggtagttgat acctccgggg cgctctttat
     4621 tatgtcccac atgggaaccc tatcccggtt tcgaggggat cgcccctgaa tggccgccgc
     4681 cttatagcct cccatagtgc ttgcggtttt ggataaagta tagtatagta aacacgtcag
     4741 cagaccacac aagggcttgt tgattttttg ttttgtggtg cccaacttta caaaaccacg
     4801 ctgttcaagg tcatgccacc ttatgatgga gaaaatagct atacaggcaa taaaaaatag
     4861 aatttaaata aaaaatataa ggagttatct caagatgatt aaaaaaatca ctttgaaacg
     4921 ctcctttttt tataattaca tcgtatcatg tgagatttca tcctcaatca acgcagttct
     4981 ttctctgtta cccatacaat gatttgtacg ttgtcttatc gtacaaagaa gctttgacga
     5041 agtttcaaat ttaactaaac tacattttac tgtttatctc ctttttaaga tataagtaca
     5101 catattatgg tatttattga aataaatcat attacactct atgtctgtag cttttctttg
     5161 aagtctattg acacagttat atgcctttgt tataataaca acaccagttg cgcaacttaa
     5221 gttaccgaaa ggaggatgaa aagatgccac caaaaccgat tattacaaaa caagatatta
     5281 ttaacgctgc aattcagttg gtaagagaaa atggaataag tagcataaat gcccgtaact
     5341 tagcaaaaac ccttaattgc tctacaaaac ccctttttcg tatttacaca aatatggatg
     5401 agataaaaaa agatattaaa atagaactag acaattacta taattctttt atggaaagta
     5461 aaataaatga tgaaaatcgt ctgttgagtc aaggacttgc atacattgaa tttgctcaaa
     5521 atgaaaaaat gattttcaat accttattta tgaatatgac aatgaagggt tcatccttac
     5581 aagatattat tcatgcagaa tggaacagaa ttactataga aaatgctaaa aaagttacag
     5641 gcttatctat agaaaaatca gaaatactat ttattaactt ttggctttat tctcatggag
     5701 tggctacaca aattatttcc aatggtatag acattccatt agatatggta caacagttac
     5761 ttgaaaatgc ttttgagcga ttttcattgg atataacaaa gaccgaataa agaaaggact
     5821 gttttatatg ttggaaagta aaataagaac acctgaagaa atcaaaaggt atatgcttga
     5881 cctcaaagaa tggttagcgc aagaaagaag taagccagtt gaagaaatgt cggatttctt
     5941 ttcaaagcga attgatattt atgataatat tcatcttggt aattgggcag aagaatatgc
     6001 acatatagca gattattttg atactccact atcttcttta cttgatattg gttgtggtac
     6061 gggtctagaa cttgcatcga tttatcgaaa atttcctaat gtacaaatta caggcattga
     6121 tttatcaaaa gaaatgatga gtaaacttaa agaaaaatat tcagataaaa atattgattt
     6181 aatcttggca gattattttg aatatccttt tgagcaagag aaatatgatg ctgccctctc
     6241 atttgaaacg cttcatcatt tcaaatatca gaaaaagcag aagatatata aaaagctttt
     6301 tcaaacaatt aaacctggag gatattatgt tgagtgcgat tatattgcct gttgtttaga
     6361 ggaagaaaca atttgtcttg aacaatatga atttaaacgt aaagaaaatc atgtgcctga
     6421 tgatgttttt gtccatattg atattccttt gactattgaa catcaaatac acttaatgaa
     6481 gaatgcaggc tttagagctg tgtatgtact tcatgaaaag aacggcacaa tgattattaa
     6541 agctgaaaaa taaactcaaa atattttcct aattgtagca tatcgcttat ggacaatcta
     6601 caaaattcat agatatgaaa tttaaaagag ttttatattc atccccttaa aaactagtgt
     6661 tctactacat agcaggacga aaaaaaaaca gtgcgtaaca ttttcttata atacgcactg
     6721 ttttcatttc aatcccgtat atcaacaatt aggttagctt taatgttaca ctacttattg
     6781 gcgcttacct aaatataact ctccttattt atgcaatcaa ataaatattt tattttttaa
     6841 gtcttaaact atatcttttg tttctagttg tttacatagt atagatattt atatcataaa
     6901 ttatataaac ttaatatttt tctaattact gtataccaaa tgtttgaaat ctatttaatt
     6961 tgccatatct cttaataagc atttcccact cttctttatc atagaaatca cacttatttc
     7021 taccaaatag cttggcaact tcaattgcaa atcttccagc tgaatctaaa tcactaaaat
     7081 tagttgcacc tgtagcacat ccagcaacag gcatctctgt tgtaattgct actcctacta
     7141 ttggtgcatc tgtagctact gctggttgta aaatactatt taagtgatac agatggtttc
     7201 catatggtgt tatatcttga atagacaaag ggaatacaaa tggagatttc cctgttacag
     7261 tctgcataat atctaaaagg ttatcactag tttttaatat gtagccttct ttaacagttg
     7321 gtgatattgc aaatcctcta gtattgatta ctctatttcc tttagtagta tctatagaaa
     7381 gtattgcatc acaactacta tcaacttcat tttcattcat agtcatcata tcaattggag
     7441 aatccataaa aggagtagga tagtgttctt tagttggagc atctgggcaa atatgagttg
     7501 aaattacaac atcaccatct aatatatccc cttttcgttg catatccaaa agtttagcag
     7561 ccactgcaat tgctaccaat gcgccatcac catctgatac aaaacctata acttctggtc
     7621 ttgctccaat ccctcctagt ctacctaaaa tgccaagagt aggagcatct ccacctttta
     7681 attttccatt tttaccagga attgtaagct ttactaaatc tgttgaacct ttacttgaag
     7741 acaattcttt tacagttaca tctacttcac cataaccttg taaataagtt tttacttctt
     7801 ccccattagc atttgcttta tctaaaatgt cataaacctc tattacttgt tttaatatca
     7861 tgtctaatcc gatttccttt cttaatatat tttaaaatta tatattattt actatacaaa
     7921 atatctactc cacacattgc tagcacctta attgctttta taagtgctac tctgccttct
     7981 tcaccaccag ctgcaacagc aaattcatct gtatgagttg cagtattttc ttttaatttt
     8041 atatatgctt gtattgcagg tatctcatga gttaaatttc ctgtatcagt tgaacctata
     8101 ccttgactta aatctctttc aacatagtct tcacccaaaa cttcaaaatt ttgtctaact
     8161 atattcacta attcagaatc attcttaatc tctttgtata tttcatctaa ttgaactatt
     8221 tctacattag ttccagtaga aattgcagct cctttagcac aatttttaag catttcacaa
     8281 atctcattta aatattctat gcttaaggct cttacattaa atatagctgt agatttttca
     8341 ggaattatat tatgtatgct acctccatca gttataattc catgcactct tgcataatct
     8401 ttaagatgta atctcatgct atttacactg ttaaatagtg ctattactcc acttaaggca
     8461 cttttccctt cccaaggaaa agcagcagca tgtgatgcct taccagtaaa atcaaatttt
     8521 aaattaactt gagcaaatga tatatcatca ggcattgaag catcacttgg atgtaaaatt
     8581 aatgcagcat ctacattatt aaatacaccc tctttaatca tagttatttt tccacctact
     8641 ctttcttctg caggcgttcc aaaaactttt acagtccctt ctatattatg ttttttcatg
     8701 atttctttta atacgatacc tgcaccaaca gaacttgtac ctatgatatt gtgaccacat
     8761 gcatgtccca ttcctggaag tgcatcatat tcagctaaaa aagcaactgt tttcccatta
     8821 gttccatttg aatacacagc ttcaaaagat gtttctaatc ctcctacacc tttagttaca
     8881 ttaaattcat tttcttctaa caaatcacat aatgcttttt gtgacttata ctcctcaaat
     8941 gctaattctg gatttgaaaa taaatagtca gaaacttttt caaatgatgt ctttttctct
     9001 tcagataata actttatttc ctctttaatt tttgctttta attcattcat atttactacc
     9061 tccttaaagc attccattta atcttaaaac tgtttgagat ataaaagcag aaccaaagta
     9121 agtagatgtc ataactataa atgatataac tactaacttc caactcaaag tctttaatat
     9181 atctaattgg ttacctacag agattcctgc aaaagctaat aatggtgtta ctgtagacat
     9241 aaaattgatt ttccctacat ttgttgctat gatatccccc actggagata ttggaagcga
     9301 taaaacgaat gcaactaatg tagcccatgc aaaacctggt aaattaggtt ttttaacaaa
     9361 attctttatt ataatagcaa ttatcaccaa caaaaatcca gatacacttc caatcaataa
     9421 atcttgagcc tgagtcttaa gtgctatcat ttgtccaaag caaataataa tatatataaa
     9481 caatactatt tttatctgct ccataatagt cttaaaagtt ttttccatat atatatcctc
     9541 ctttacaatt tattacttgt tcttcttttt tttcttcctc taaatctttc aataactgga
     9601 gctactttgc tataatagta gttggctacc ggtaaagata aaaataattc tatatatatt
     9661 ccagtaacag ctgtaatcaa tccactagta gctgaatatg ctaaaatctg ctctgacatt
     9721 tgtggaaccg tattcattaa tgatgctgtt gcagctccca tcattgaacc actacccatt
     9781 cctgatgcca ttgctaaagc atatggatga aatcctaaaa gtacacccac tggtgctaaa
     9841 aatgaaaata ataatgttcc aaatatacta ccacttatgt aagttcctag tgttcctata
     9901 ccttctggtg agtttaatcc atacttatct ccaataattc ctagtgctgt gtcacgacac
     9961 aatgaaaaag taccacctaa tgcacttctt ccaagaccta atgctactcc aacaggtaat
    10021 gctatcaaca ttgtacccat atgaccaaat tcttgtaata aaagtgctgg tcctgcttga
    10081 agtaatatac caactttagg ccctgcatta attccaaaca ttatccctaa tggatatagt
    10141 gtcaaagata ataaatatgg agatacattt atttcattat cccctataaa cttttttaaa
    10201 gctggtataa ctctccctaa taaatgagga gaaacagcca ttcctatgaa tatagcccat
    10261 agtatactaa gtaaagttac attaattccc ataactttaa atgaaatagg tccaattaat
    10321 tctgccaata ttgtgattat aaaggctgct attatggcat ttctaatccc tgctttttct
    10381 ttcatttcta tacctccttg taatttttat aattttctta taatataatt gttagtgatt
    10441 ttat
//
LOCUS       DAEMAE010000128        10417 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEMAE010000128 DAEMAE010000000
VERSION     DAEMAE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.130"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            366..1526
                     /locus_tag="KPH93_002500"
     CDS             366..1526
                     /locus_tag="KPH93_002500"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG1689307.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNAEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1528..2658
                     /gene="glgD"
                     /locus_tag="KPH93_002501"
     CDS             1528..2658
                     /gene="glgD"
                     /locus_tag="KPH93_002501"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG1689308.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2687..4129
                     /gene="glgA"
                     /locus_tag="KPH93_002502"
     CDS             2687..4129
                     /gene="glgA"
                     /locus_tag="KPH93_002502"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG1689309.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQTYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4143..6563
                     /locus_tag="KPH93_002503"
     CDS             4143..6563
                     /locus_tag="KPH93_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG1689310.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIAELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQNRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6568..8433
                     /locus_tag="KPH93_002504"
     CDS             6568..8433
                     /locus_tag="KPH93_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG1689311.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELNICVKVNEGYNVKSIFL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQTYKSWWDVKTLPNINELEHSYMDYIIYDK
                     DSVINKWINMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFKSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYKDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRILILVN
                     RSENVESISLCMESSFIEEIPIKYSLKNVNSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8678..9331
                     /locus_tag="KPH93_002505"
     CDS             8678..9331
                     /locus_tag="KPH93_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1689312.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            9427..10026
                     /locus_tag="KPH93_002506"
     CDS             9427..10026
                     /locus_tag="KPH93_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HBG1689313.1"
                     /translation="MYPNKETAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEHCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKKQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4208 a         1016 c         1883 g         3310 t
ORIGIN      
        1 ctttggataa ttatttaaca aaaattagat tatattttgc tattacgaat ttaacaaata
       61 agcatgttta tatgacacaa aagatttata aaaaatgttt ttatatttat aaaataaaat
      121 ttgtattatt ttttaaccga taattgaata ttaataaata ctatagtgct tatggaaaat
      181 aatgtatacg ttggtaagtc cattatgggg tatacattat atcaattgta tagttgatat
      241 tattttattt ttattaaggt attatttaag caacatttat acacgacatt tattgactga
      301 taaagtttat aaaagtaaaa atgcaattat aaatattaat tactagatga ggggagtgtc
      361 attaaatgaa aaaagagatg ttagctatga ttttggcagg agggcaaggt tcaagattag
      421 gtgtttttac aaaaagaatt gccaaaccag ctgtatcatt tggaggtaag tatagaatta
      481 tagattttgt gttaagtaat tgttcaaact ctggtattga tactgtaggt gttttaactc
      541 aatatagacc gttgatatta aattcacata taggcatggg aagtcattgg gatttggaca
      601 gaataaatgg tggtgtctat gttttacaac catttatgaa tgaaaaagaa ggaaattggt
      661 ataatggtac agcacatgcg atatatcaaa atatggactt tgtagatacc tataatccag
      721 aatatgtgtt gatattatct ggtgaccata tatataagat ggattatagc aaaatgttaa
      781 aatttcataa ggaaaaaggc tctaaagcta caatagcagt gattgaagtt ccttgggatg
      841 aagcttcaag atttgggata atgaatacaa atgaagattc aagtatctat gaatttgaag
      901 aaaaaccaag tgaacctaag agcaatttgg catctatggg agtatacata tttgattgga
      961 aaatgttgag aaattatttt aaggaagcag aaaaaaatgc agaaataaat tatgatgatt
     1021 ttggaaaaaa tttaatacct aaaatgttag aagataatgt tggaatgtat gcttatccat
     1081 ttaaaggata ctggagagat gtaggaacta tacaaagtct atgggatgct aatatggata
     1141 taataaaatc tccagaaaca cttgatttag cagaccctaa gtggaagata tatacaaata
     1201 ctatggcaat gcctccacaa tatataggta aaaatgcaaa tgttcataga tcaatgatag
     1261 ctgatggttg tagaatttta ggtgaagtag gtaactctgt attatcacat ggagttgttg
     1321 ttggtaaagg aagtaaagta attgattcag taataatgcc aaatgttgta ataggtgaaa
     1381 atgtaactat agaaaaagct atgataggtg agtgtgcaac tataaatgat aatgttcaga
     1441 taaagaatgt aaataatgaa ataaatgtag tttctgagta tgaaaatatc gaacctagat
     1501 gcgtattaat agaaggaggt ctttaagatg agaaatgaat gtttagggat aataaattta
     1561 aacaaaaaag gagacccagc aataaacaaa cttaactatg gaagaccaat agcatctaca
     1621 ccaatagctg gaagatatag aataatagac tttgctttat ctaatatgat aaattcagga
     1681 atcactaaag ttggaatatt cgcaaaggaa aaatacagat cattgacaga ccatataggt
     1741 agtggtaaag attgggattt aagtagaaaa aaaggtggcc tatcaatttt tagtccagaa
     1801 aacactaagt atagaaatac ttactcacat agagaaggtg atatatatac tatcttagct
     1861 aatctagatt atatagaaaa gagtgaagaa gaatatattt taatagctcc aagctatatg
     1921 ctatgtaatt taaattattc tcaagcattg gaataccata aaaaatctca taatgatata
     1981 actataatat ataaaaatgt aaataatgca aataaagatt ttgctggtaa cttaacttta
     2041 aatttagata gtaacaatag ggtaataaat gtaggaaata atttaggcaa atttcctaga
     2101 gcaaacatat gtatggagac atatattatg aagcgtgaag attttgttga atgtatatat
     2161 aacattgtaa ataaaggcag ttattgttat ttagaagaat ttattattga agaagcagaa
     2221 aatatgaaaa taggagccta tgaatataca ggatatctaa aatgtgtaaa ttctgttgaa
     2281 tcatattttg aaatgagcaa agatttatta aatttagaag ttgcagatga attattatat
     2341 tctgaaagaa agatatttac aaaagaaaag aatgaatcac ctacaattta tacagatagt
     2401 gctaaagtag aaaatagctt tatagcctct ggttgtttga ttgaaggtac tgtaaaagat
     2461 agtatcatat ttagaaaggt aaatgttgaa aaaggaacag ttatagaaaa ctcaatagta
     2521 atgcaaaatt gtgtaataaa gtcaaatgca aaactatata atgcaatatt agataaaaat
     2581 acgagtgtat caaatgggaa agagttaaga ggagatgaaa aatatcctct tgtagttgaa
     2641 aagaatacaa atatataaat aaatttttta gaagggggaa tgactaatga aggtttttta
     2701 tgtaacagca gagtgttggc cctttgcaaa aactggagga ttaggagatg tttcttatgc
     2761 attgcctaaa gaattaaaaa aagaaggtgt agatgtaagg gtaataatgc ccaaatattc
     2821 aactatacca agttatttaa aagatcaact aaaagaaata gctgtgttca gtgtacgtgt
     2881 tggatggaga aatcaatact gtggtctttt agaaatggaa ttggatggtg taaaatttta
     2941 ctttatagat aatgaatttt attttagaag agaagatgaa agaaagagta tctatggata
     3001 tggagatgat gcagaaagat atactttctt tacagatgca gttttagaag ctataagtag
     3061 aattgatttt tatccagatg taatacatat aaatgattgg catacgggga tgttaccatt
     3121 aattctaaaa gaaagatatg caacccttga ggggtataaa aatataaaaa ctatgtatac
     3181 tatacataat ctccaatatc aaggtgtatt tgataagcat gttttatatg atatattgga
     3241 cttaccacaa acatactttg ataatggaga tatagagtat tatggctcaa taaattttat
     3301 gaaagctggt ataaattttg cagataaaat tataactgtt agtcctactt atgcaaatga
     3361 aatacaaaca tctttttatg gtgaacaatt agatggatta cttagaaaag agtcaggaaa
     3421 attaaaaggt atattaaatg gtatagatta tgatttaaat gaccctgcaa aagataaaga
     3481 tatatttgtt cattatgatg tagactcaat tgataaaaaa gtagaaaata aattgagatt
     3541 acaagatata ttgggactta aaaaagattc atctattccg ttgataggta tagtttcgag
     3601 attagtttct caaaaaggat ttgatttaat agcatacatg atgccagaac ttatgagaga
     3661 agatttacag attgtcgttt taggaactgg agaacatcaa tatcaatcaa tgttcaacta
     3721 ttacgattct aatttttcag ataaagtatc agctagaatt actttcaatg catcattggc
     3781 tcaacagata tatgctgcaa gtgatatgtt cttaatgcca tcattatttg agccttgtgg
     3841 tataggtcaa atgttagcta tgagatatgg ttctttacca atagttaggg agactggagg
     3901 gcttagggat acagtaacac cttataataa atttactgga gaaggcaatg gatttagttt
     3961 taaaaattac aatgctcatg agatgttttt ctgtttgaaa aatgcaatta aagtgtttaa
     4021 ggacaaagaa aaatggatta aattagttga aaatgcaatg aaaacagata atagttggaa
     4081 aaaatcagct aaggagtata tagaaacata tagagacata tgtgattaaa ggagtggttt
     4141 ttttggtaac aataagcaaa aagaaattca agcaaaaatt tgaagtaaaa atgtatagct
     4201 tatatgcaca atctatcaag gaagctactg atgaacaatt gttaaatgta ttatgtagtc
     4261 tattgaaaga tgagatagca aaaaaatggg ttgctacaaa gttagataag aaaaaagaag
     4321 tttattattt tagtttagaa tttttaatag ggagacagtt aaaatctaat ttattaaatt
     4381 taaatattga agaagaagtt agagaaggtt tatctgaatt aggaatcaac ttggatgatt
     4441 taattgaagc tgaagtagac ccagccttag gaaatggtgg acttggtaga ttagcagctt
     4501 gctttttaga ttctatggca tctttgaaca taagtggtca aggatatgga ataagatata
     4561 aatatggtct gtttgaacaa aaatttgtaa atggttatca ggtagaagtg cctgataact
     4621 ggcttacaga aggacgatat gcatgggaaa ctgtaagacc aaatgaagca acaatggtga
     4681 agtttggtgg tgaggtagaa ttaatcaagg aaggtagtca tttaaaagta attcataaaa
     4741 actaccttcc tgtaatggca atgccttatg atataccaat aatcggatac caaaaccaat
     4801 gtataaatac tttaagattg tttaagagtg aaataccaaa gagagatttt ggtgaactta
     4861 cctcaaatgc tctaaattat tctggtagct atgaagaggc tttaaaacat aagtattata
     4921 ctgaggaaat atcacaggta ttatatcctg atgattcaaa ctatgcaggt aagttgttga
     4981 ggttgaaaca agaatacttt tttgtaagtg cagggataca agatataata agaaaatata
     5041 aaaagaacaa attaaatatt aataatttat tcgataaggt agcaattcat ataaatgata
     5101 cacatcctac cttatgcata gcagaactta tgagaatttt gcttgatgaa gaaaatttat
     5161 cttgggatga agcatgggaa ataactaaaa aaacagtatc ctatactaat catactataa
     5221 tgtcagaagc aatggaaaaa tggcctgtaa gtatgatgaa agaactatta ccaagaattt
     5281 atatgataat agaagaaata aatagaagat atgtagagga attaaataat aaaggatatg
     5341 accaaaatag aataaaaaga atgagtataa tagactgtga caatatcaat atggcaaact
     5401 tatgtattgt cacaagtcat agtgtaaatg gagttgctaa gcttcatact catattcttg
     5461 aaacagaggt attaaaagat ttctatcaag atgaaccaaa taaatttaat aataagacta
     5521 atggaattgc acataggcga tggcttataa gttcaaatcc tcaacttagc aatttaatta
     5581 cagatttaat tggtgattca tggaagaagg ataccttaca gttaaaaaat attgaaaaat
     5641 ttaaaaatga ttcctctgtt ttacaaagac ttgatgatat aaaatacaat aataaagcaa
     5701 atctagcaaa gtttataaaa gataaatacg atttaaatgt agacccaagt tcgatatttg
     5761 atgttcaagt taagagattg catgcatata aaagacagtt actgaatata tttaatgtac
     5821 ttcatatgta tcatgaatta cttgataatc ctaatttgaa tcttgaccca agaaccttta
     5881 tatttggcgc aaaagcagca ccaggatatt atttggctaa atgtataatt aaatttataa
     5941 attctgttgc aagtactata aataatgatg ttagagtaaa ggacaagtta aaagtagtat
     6001 ttttagagaa ttatggagta tcattagcag aaataataat accagcagca aatgttagtg
     6061 agcaaatttc aaccacaaca aaggaagcat ctggaacaag taatatgaag tttatgatga
     6121 atggtgctat aactttggct acacttgatg gcgcaaatgt agagatatgt gaacaggttg
     6181 gtaaagaaaa tatgttttta tttggactta gtgcagaaca agtactaaat tataataaat
     6241 atggtggata ttcttcactt gacctatatc attctaatat ggatataaaa agggtagtag
     6301 atgatttaat aaatggattt attcctaatc ttggcgaaga aggaagaagt atttataact
     6361 cactcactac ttacaatgat gagtattttg tattgagaga ttttgaaaat tatggtcaag
     6421 ctcaagcaga tataaacagg ttatatagag ataaagaaaa atggaataaa atgtctttag
     6481 ttaacatagc taactctgga tttttttctt ctgatagaac aatttctgag tatgcaaaag
     6541 acatttggtt taaaagggtg tgattaaatg caaagtgtaa tagaatataa ctcttgggat
     6601 aaaaatttta aggctccgtt tggagcttta aaatttgatg aggaattgaa catttgtgtt
     6661 aaagtaaatg agggatacaa tgtcaagtct attttcctag aaataaatag agaagaagaa
     6721 atgagaacaa taactttaaa tgaagaatta gataatgata aacttggaaa gtgcttttgt
     6781 ggtaagattg aaaagtttga tgggacaggt gtttattttt attactttaa agttgatgtt
     6841 gaaatggatg gtcagataaa gactttattt tatggaaaaa acagagacaa cggatactct
     6901 tgtgaatata attatagtga tattaataaa tatcaaatca ctgtttacaa agattttaaa
     6961 gtaccaacat ggtacaaaga aggaatactt tatcatatat ttgtagatag attcaataat
     7021 ggaaatcgta gtggtaaggt tgataatcct aaaaaaaata gtttcatata tggaaattgg
     7081 gaagatatac ctatgtacat aaaggatagt caaggggatg ttataagatg ggatttccat
     7141 ggtggaaatt taagagggat aatcaataaa cttggatatt taaaaaaatt aggtgtaagt
     7201 atattatatc taagccctat atttgaagcc tctagtaatc ataagtatga cacaggtgac
     7261 tataaaaaaa ttgacccaat gtttggggat gaagatacat ttaaagaact tattgataaa
     7321 gcaaaagaaa aaggtatatc aatagtatta gatggagttt ttagtcatac tggagcagat
     7381 agtaaatatt ttaatatgta tggaaactat aatagtttag gagcctatca atcaaaagaa
     7441 tctccatatt attcatggta tatgtttgag gaatttcctc aaacatataa aagttggtgg
     7501 gatgttaaga cattaccaaa tataaatgaa ttagaacata gctatatgga ttatataata
     7561 tatgataagg atagcgttat aaataaatgg ataaatatgg gtattaaggg ctggagacta
     7621 gatgttgcag acgaactacc aactaagttt ataagagagt taaaaaagga attaaaaaag
     7681 gcagatgatg actcaattct tataggtgaa gtatgggaag atgcatcaaa taagattagc
     7741 tatggtcaga gaagaagtta tttattaggt gaagaattag attctgttat gggttatcct
     7801 tttagaaata acatgttttc tttcttaaaa ggagaaatta attcatacga actttgtaat
     7861 agatatatgc agattaaaga aaattatcca aaagaatcat ttaagtctaa tttaaattta
     7921 attggaacac atgatgttac aagagctaaa acagaattaa atgatgatgt agatttagtg
     7981 aagcttgctg tagctataca gatgacattt gaaggagtac catatatata ctatggagat
     8041 gaagcaggtc tttgtggagg tgttgaccca gataatagaa gaacatatcc atggaaaaat
     8101 gaagatgaag atatgcttaa tttttataaa gatgttataa aaattagaaa taagaataaa
     8161 cttttaagtt ctggaaatac agaatttata tatactaata atgattctgt attttccttt
     8221 attagagtta atgaaaataa tgatagaata ttaattttag taaatagaag tgaaaatgtg
     8281 gaaagtatat cattatgtat ggagagtagt tttattgagg aaatacctat aaagtatagt
     8341 cttaaaaatg taaatagtac tatacaaatt gaaaataatg agttgaaagt agatttagac
     8401 tctaaatcat ttagaatttt tagagtaaat taaatatatt tgtttagaaa aataaataga
     8461 aaataaggtg aatgtggaaa gagttttgtt ttatcaaaac tttttctact ttttttgtat
     8521 aaattatcat aagaatttta gtatttcaaa atattacaaa gaagtataaa aaatatcata
     8581 attgtgatat tatataaatt agtaagataa aactgaactc tctaccaaag ttgtagggag
     8641 atttaacttt tataatctta atatggagga acgtcttatg aaggtagatc cacttacaac
     8701 tcaagtatat gactatatat caaagaaaat ccaaaatgga gagtatgaag ctaatcagag
     8761 aattactgaa tctgagattt gtaaatctat cggagttagt agaacaccag cgagagaagc
     8821 attaactagg ttagcaggtg aaaatttact agaaaaaata cctaataagg gatttgtagt
     8881 aaaagaattt caagaaaagg agaaattaga cacctattct gtaattggag tattggatgc
     8941 gttagccggt tcttcagcat tacaaaattt aactgaatca gacctagtaa aaatggaaga
     9001 attaacagaa atgatggcgg tctctataaa gtataaaaat tacaatagct atttaaaatt
     9061 aagtaatgaa tttcacgata tatacataac taaatcagat aatcaggtgt tagtaaatct
     9121 attgaactct ttaagatata attttatgtc aaagtcatat actagtgata atgaagatga
     9181 gttatataaa atgttgacat acagcaacaa tcagcatatt gaagtagtaa aattcatgag
     9241 ggagaatgat ttagaaaatg ttgaaagagt tttgagagaa cattggaaaa cgatacctat
     9301 agcagaaatg aaaaaagaga agcttttata aagtttctct ttatttttta taccatttta
     9361 tcactatttt tttgaattat taagtagaaa ataataatta atttaaattt gaggaggaaa
     9421 actaatatgt atccaaataa agaaactgca atagaagagt tagaagtggc tggcaaaatg
     9481 aatcccggtc catggataaa acattcttac aatgttgcta atgtagctaa attgattgca
     9541 gagcattgtg aaaatttgaa tagcgaaaag gcttttgtgt gtggattatt acatgacatt
     9601 ggcagaagaa ctggtatttc ttctgtaagg catattattg atggatatga ttatgttatt
     9661 tctaagggat gggatgaagt ggcaagggta tgtttgacac attcttttcc tgttaaggat
     9721 atagaagctg atattggaaa taaagatatt agtaaaaaac agtatgattt tatcaatcac
     9781 tatttaaaca accttgaata taacgattat gataagttga ttattctttg tgatgcatta
     9841 gcagatgcaa atgggttttg tattttagaa aaaagatttg ttgatacaac acgaagatat
     9901 ggaatatatc cgttttcagt agaacgatgg aataagactt atgagtataa agaatatttc
     9961 gagaaaataa tcaataagtc tatatataca ttgttaccta atattgaaaa atctatatat
    10021 tattaaatcc taagttaata acttaatatc cagtatcacc agcagttggt cattatgatt
    10081 gactgctttt attctgctta tattaaaaaa atacatcatg gtttgtgtat atcaaagtta
    10141 tatggagtga aaagagaatt tattttatca attaaaaaat ttaataaatt ttatattaaa
    10201 attaaaatac aaaaatagga aaattattta gtccaactaa accaagaatt ttgatatatg
    10261 aaataagaat aatataaaag tttagtaata aaatacttac aaattaaaaa tagtaaactc
    10321 aaaagcttac taaaactcaa aaaagtaaaa atgtcaaaac aataaaaaat aaaaaagttt
    10381 ataaatacta agttttaaaa aaattaatat aaacaat
//
LOCUS       DAEMAE010000129        10374 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEMAE010000129 DAEMAE010000000
VERSION     DAEMAE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.131"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1896)
                     /locus_tag="KPH93_002507"
     CDS             complement(<1..1896)
                     /locus_tag="KPH93_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1689314.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKALSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILLKYNLILVSKPYYGVKIEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNIDLIELQKVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYNNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVIPSFTESVFYRESLSSTNIDDFLAIPHPMELS
                     SIKTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFD"
     gene            2131..2583
                     /locus_tag="KPH93_002508"
     CDS             2131..2583
                     /locus_tag="KPH93_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1689315.1"
                     /translation="MVLQEKNIFIDKNLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HENEGNIHLKTLSKLATCLMEDDFISEVKNASDASHLVKYITNKMKEE"
     gene            2586..2894
                     /locus_tag="KPH93_002509"
     CDS             2586..2894
                     /locus_tag="KPH93_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG1689316.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHKIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3011..4117
                     /locus_tag="KPH93_002510"
     CDS             3011..4117
                     /locus_tag="KPH93_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG1689317.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYILPLIIGSSLVVAIPKLIALAM
                     GITSLDAYKNISGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSAAGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4192..6870
                     /gene="mngB"
                     /locus_tag="KPH93_002511"
     CDS             4192..6870
                     /gene="mngB"
                     /locus_tag="KPH93_002511"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG1689318.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQKRKLVIGPWYTQTDQLVISGESIVR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDTIGKLKVRASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVVKDIWRLMFENT
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGL
                     PEVRNEVVEFETYIPGQDFVIKNKNGEILNYIIKEKEDISDYVLTQTIRLNPSKKIYI
                     PKNVYRAKIALSVKDIPPMGYTQLVFELDTKNKCKVKTSNFKEIENKYYKITANKNGS
                     VEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     ILLKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNDQALSHRVRV
                     LFNTEIASKFSIADQQFGVIQRPTVLEKDLVLWKRDNYSWQEKPITIEPMQSFVTLEN
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGVVVRIFNGMKNEDAKGSLNINKNINGAYS
                     AMLDEIYDNTNKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7192..9525)
                     /locus_tag="KPH93_002512"
     CDS             complement(7192..9525)
                     /locus_tag="KPH93_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1689319.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFESWESFIKESRKNLKYYLVDKESKFEFFNDNKELGALD
                     TTKNDVSKEDSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNVVDSYG
                     VNQKVINQMLLSKSREDLMDIDLGGNTISYKLSPIKNMTLIYAVPKVISKGDSREYSY
                     GNMYSNDNISSYINNVENNSYYEISRMVLKIILAIVVIVALVFPYRKSKELLGFDTLS
                     RIPLEILFIILAIIQGIVDLYPTGIISDTLNGNYVEYLINNDISAESASILVSLMNLT
                     YWFVIFSIIFVFITLLKHILNVGIREYFIKNTVTGIFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVIILGINLLIIVIMCSMWFFGIILALIYSAVLFKILSDYSKKTICEYNQLL
                     DVTKKISDGNLEVNMEKDLGFFNPIKEELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VHLNIVNVDIVSLMKQTLLELDDKLADSSLILKNNFSSEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNRVNIMLKNMSATEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9500..10216)
                     /locus_tag="KPH93_002513"
     CDS             complement(9500..10216)
                     /locus_tag="KPH93_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689320.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3859 a         1447 c         1346 g         3722 t
ORIGIN      
        1 atcaaaatca ttacaattag atatactatc tctaatatca ttgtcatgaa ttatctttaa
       61 aaggatatca taaatggagt ttatttttat ataatcatct ttatttatag ccaacatcat
      121 gattaattta acagtagaat cttcactcca atatattggt tcattcaata tggaaataca
      181 aatcttagtt tttatagagc ttaattccat aggatgaggt atagctaaaa aatcatctat
      241 attggtagaa gataaacttt ctctgtagaa tacagattca gtaaatgatg gaattacaat
      301 ttcactttga gttaatttgt tgcacatcaa ctttataagc tcatctttat ctttgatttt
      361 aggattaaca ataaataaat ttttatcaaa gaaattatcc aaaagattat tataaatatg
      421 agaattatta gtgattgact tagatatatt ctctatatct ttttttaata gttttaaatc
      481 tactaataca ataggaatac tatcatgatt taaaggaatt gtcgatatta ttatatctac
      541 atctgacaat tcactagcta aaaattgatt aaatgataga cattgaagta tatttatttt
      601 atcatgaaaa actttgttta attgaacctc taataaccta cttgaaccat accctgaacc
      661 acatactaat actacgtttt tacacttaat attgttttga aaaaatctct ctattccagc
      721 acctatatgc agggatacat atccaatttc gtcttctgtt aaagagtaaa tactattttt
      781 aaaaactttt tctatagcat ttaaagttat ttcaaatgct aaaggataat tactctttat
      841 tgtatttata aggggattca ccttatttaa attataagat ttagaattaa gaattgattt
      901 aaagtgcagt actaaatcat caaataatac actatcattt ctaaggtcaa aagtatattg
      961 attgttgatt acttctaaaa tttcttcaac atactctata attttagtat ccactctatt
     1021 ggaaacgtta tccaaaagag aacttttagt tataaaatga ttatatagat aaactctatc
     1081 tgcctttgaa ataattatag tatatttact ctcaatataa ttaaatatat tttcaacagt
     1141 tgtattcgat tgaaaatctg ttaaaacaac atctaaaaca ttatctaagc agtaaccatc
     1201 aagtattcta cttatagtaa tagctaaatg gataataaag ttttttagat taaaatctag
     1261 gaaatttata ttaaatttat taatttcact taacactacc ttctgaagct ctattaaatc
     1321 tatattatta aataattcta tttctctatc tgtaattcca attatatagt tttcaaagtt
     1381 cctatttatc atattattag aaatgcatct tcgaatatct atttccttcc cttcaatttt
     1441 aactccataa tatggcttag aaactaatat taaattatac tttaacaaaa taggctttat
     1501 tctttttata tcatttagta ttgtaaattt actcacatac aagctattag ctaaatcttc
     1561 tagttttata tatgtattgt tataaagaat atattttaaa atatattgat ttctttctat
     1621 aggagaatct ggtatttcac ttgaatccat aatatcatca gatattaatg ctagatactg
     1681 ttggtataat gctaaattaa gaatatctat ataatatcct tctccttttt ttattttaat
     1741 aatagcacca tttttctcta aagttccatt tatagaagta atatcatttc ttatagttct
     1801 ctcactcact tttaaatgat tacttaaagc tttacaagta attttatcat cactttcatt
     1861 taatagttta aacaacgtga gcaatctctt gtgcataaat atcccccctt atagaatttc
     1921 ttctaaatta tactatttac actcatataa tacaattact ctaataaaat tgtattataa
     1981 taatttattt ccatttcgac atggaaatta gttattatca taatataaat ttccatgtcg
     2041 aagtggaaat tagttatatt taatttaatt aggataacga ttacaatata aatataagtt
     2101 aagacattac gaatggagtg gaaaaaatat atggtactac aagaaaaaaa tatatttata
     2161 gataaaaact taaaaagcaa agaggaagta ttaaatttta ttgcagatag tgcttatata
     2221 ctaggtatta caactaataa agaggctctt ttaaaagacc ttctaaaaag agaaaatgaa
     2281 tttccaacag gaataaaaga tggatttgcg atacctcata caaaaagtgt aaatgtaaag
     2341 gaagcttcta tactctatat taaaacaact aacgagttag agtggaaaac tttagatgat
     2401 agtaaggtta gatatgtatt tagtttgtta gctccacatg aaaatgaagg aaatatacat
     2461 ttgaaaactc tatctaaatt agccacatgc ttaatggaag atgattttat aagtgaagtt
     2521 aaaaatgcta gtgatgcaag tcatttagta aaatatataa ctaataaaat gaaggaggaa
     2581 taattatgaa aatagtagca ataacatcat gtatagcagg attggcacac acacctatgg
     2641 cagcaaaagc tttagaacaa gctgcatcaa aaatggggca taaaatcaaa gtagaacaac
     2701 aaggtgctat gggacgagta aatactataa caaaagaaga agcacaagat gcggacttta
     2761 tacttatagc atctgaccaa actatatctg gaatggaaag atttgatggt aaaaaagtga
     2821 taaaagtcaa gataggtata gcgttaaaaa aaccagaagc tgtcttaaca aagtgtatag
     2881 aagctatttc ttaataaaaa aaatctcact ctaatataga atgagaaaat ttaaaaattt
     2941 aaaaattgtc taatgtaatt ataacatatt atcaaaaaaa tataacaaac ttatatagga
     3001 ggaattaaaa atgagtaaaa aaaataaatc tcttttaagt gaacttaaag gacacttaat
     3061 gacaggtatt tcatatatat taccacttat aataggctca tctttagttg tagctatacc
     3121 aaaacttata gctttagcaa tgggtataac aagtttagat gcttataaaa atatatcagg
     3181 tttttatcat attctttact taatggaaca agttggttgg actggaatag gtctgttaaa
     3241 cacagtattg gcagggttta tagcattttc aataggtgaa aaaccagcta tgggcgcagg
     3301 tttcattggt gggctcatag caagtaatac taatgcagga ttcttaggag cagtaatagc
     3361 aggtttcttt gctggatatg tttgtaaatt tttaaagaaa aaaattaaaa tatctggttc
     3421 agcagcagga atgatgccac ttataataat gccacttata acagttggac taactggttt
     3481 tttgatgagt gttgtattag caggaccttt aggaaatata aatacttcat taaatgcttg
     3541 ggtagctgaa atgtgtcaaa atggaacaaa ttcagtagta ttggctttaa ttttaggtgc
     3601 aatgatagga ttcgatttag gtggaccagt aaataaagca gcttggatgg caggaaatgc
     3661 attattgtta gaaggtatct atctaccagc tatattagta aactgtgcaa tagtcatacc
     3721 tccactagga tatggtttag ctacattcat aagaaaatca aaattttcta atgcattagc
     3781 tgaaacagga aaaggcaata tagttatggg tataattgga ataacagaag gtgcaattcc
     3841 tttcacttta acaaatccat taaaattagt tcctgttaat atgataggat gtgctttggg
     3901 tgtaggatta tcagctttgt taggtgtaca tgctataatg cctccagttg gtggattata
     3961 tggatttata tcaataggtt ctggatgggc ttacttagta ggtgcaatat ttggagcttt
     4021 agtaatagca atattatcaa caactttagt agacttcaat gaagatgaaa ctacggaaaa
     4081 tacagaattt gatgaaatag agttagatat aatgtaaatt aaaaacttaa ttttatgtaa
     4141 ttgagaattg agtgtactct caattctcaa ttacattttg ggaggatttt tatgaagaag
     4201 acaaaagtac atgtagttcc acacagtcat tgggatagag aatggtattt tacaacatca
     4261 aggtcaaaaa tatatctaat gaaagatttt caagatatat tagacatatt ggaagaaaaa
     4321 gaagacttta aatactttac tatggatgca caagcttctc tcttagatga ttatttaaaa
     4381 tggagacccc aggatgaata tagaataaga gatttagttc aaaaaagaaa attagttata
     4441 ggaccttggt atactcaaac agaccaattg gttatatcag gagaatctat agttagaaac
     4501 ctttactacg gaatagatag atgcaatgaa tttggtgaac ctatgagggt tggatatgta
     4561 ccagattctt ttgggcaatc agctcaaatg ccacaaatat atagaggttt tggaatagat
     4621 agttcattat tttggcgtgg agtttctgat gatatggtgg atacaacaga atttatatgg
     4681 aaaggttctg atggttcaaa agtattagca gttcaaatac catttggtta ttactatgga
     4741 ggtaatatac cagaagatga ttcagattta aaagaatatt taaaagatac aattggaaaa
     4801 cttaaggtta gagcttctac taataacgta tattttccaa atggatttga ccaagcacca
     4861 ataagaaaaa accttcctga tatattaaaa aaagctaatg aaatagataa tgaaaatgaa
     4921 tacgaaataa gtgatataga aaactatata gattcagtaa gaaatgaaag aaatgaattt
     4981 acagagctta aaggtgaatt tttaaatgct aagcatatga gaatacacaa atcaatattc
     5041 tcctcaaggt cagaccttaa gataatgaat aacaaattag aaaattatat tacaaatgta
     5101 cttgaaccat tgttaacact aagttattca cttggaaatg aataccctca cctagttgta
     5161 aaggatatat ggaggcttat gtttgaaaat acagctcatg acagcatagg ttcttgtaat
     5221 agtgacacaa caaatgaaga tgtatacaca agatataaac aagccagaga tttgagtata
     5281 aatttattag atttgcatat gagacttata agtactcaaa tcaaaaataa taatgaagaa
     5341 ataacactaa cagtattcaa tggattacca gaagtcagaa atgaagttgt agaatttgaa
     5401 acttatattc caggtcaaga ttttgtaata aagaataaaa acggagaaat tcttaattat
     5461 ataataaaag aaaaagaaga tatttcagat tatgtattaa ctcaaacaat aagattaaac
     5521 ccaagtaaaa aaatatatat accaaaaaac gtatatagag caaaaattgc actttcagta
     5581 aaagatatac caccaatggg atatactcaa ctagtgtttg aattggatac taaaaataaa
     5641 tgtaaagtaa aaacaagtaa tttcaaagaa atagaaaata agtactacaa aataacagcc
     5701 aataaaaatg gttcagtaga aatattagat aaagaatcta ataagactta taaaaaccaa
     5761 gctgtaattg aagacaatgg agatgatgga gattcataca actattctcc tccaagaaaa
     5821 gatatttatg tttcatcttt agattctgta tcttcagtag aagtgctaaa atcaaatatt
     5881 caagaagaaa ttctacttaa atattcattg actataccag cttcactgga agaaagggcc
     5941 ataggtaaag tttctgtgaa aatgccagta acaatgaaaa ttaccttaga acctaaagaa
     6001 caaataatta agtttaatgt taatataaaa aatgaccaag cactatctca tagggtaaga
     6061 gttttattta atacagaaat agcatctaag ttttctatag cagatcaaca atttggggta
     6121 atacaaagac caacagtatt agaaaaagac cttgtattat ggaaaagaga taattactca
     6181 tggcaagaaa aacctataac tatcgaacca atgcaaagtt ttgttacttt agagaatgga
     6241 caaagaggaa tagcattaat aactggttgt gtaagagaat atcaaatagt tggtgataat
     6301 ttagatacaa tagcattaac actattcagg tcatttggat atatgggaag agaaaattta
     6361 ctttatagac caggaagagc ttctggagaa aaaataatag caacaccaga tgcacaatta
     6421 cttaaagagt taaactttga ctttggtctt tacatatata acaataaatt tgatgaagca
     6481 aatgtagcta atacagctaa gagattctta acacctatac aaatatatga atatgctgat
     6541 tttttaaatg gtagacttat atttgcattt aatgacgaaa aacaaatata tgaaaatgaa
     6601 tatagtttga tgaatgtaaa taattcaaat ttcacagtaa gtgcaataaa gaaagaagaa
     6661 aaaggagacg gagttgtagt tagaatcttc aatggtatga aaaatgagga tgctaaggga
     6721 agtcttaaca tcaataaaaa tatcaatggt gcttatagtg caatgctcga cgaaatctat
     6781 gataatacta ataagcttaa agtaaatact aaaactgtag aagtaaattc tttgtcacat
     6841 tgtaaagtac aaacaatagt aattaaataa tataaaatag cttatacaag atttcaaaag
     6901 aaattcctaa ccttgtatta atagtaaata caagtttaca ctatttagaa tatctatgac
     6961 actaaataac attttgcaaa ataaaaagat gaatcctaat atattaggat tcatcttttt
     7021 taaataaatc agcattctgt tttgaaataa gatttacaag ttgttttgaa ataatgctta
     7081 caaattattt taaaacaata cctataaatt tttatttaaa gaatacacat aaagatttcc
     7141 agactctttt attctataga ctttataatt attaaagaag tctaatttac tctaattttt
     7201 gttaaaagta attgtaactt taaataaatc accatccaca cttatattca tcttaccacc
     7261 ctgtaattct ataaaacttt ttgctattgc taaaccaagt ccagaacctt ctgtgttacg
     7321 tgacttatct cctcttacaa atctctccat tatatcatct acactaaaat caatctcagt
     7381 tgcagacata tttttaagca taatatttac tctattgtct gtttctaaaa tatcaatata
     7441 cactcttgaa tttggcattg catatttgct tatatttatt ataagatttt caaatactct
     7501 aaatgtacgc tgactatcta gtggtaatat tattttctca ctagaaaaat tattttttaa
     7561 tattagagat gaatctgcaa gtttatcatc taattctaaa agtgtttgtt tcattaaaga
     7621 tacaatatcc acatttacta tattcaaatg aacatctcca ctatttgcct tacttacttc
     7681 gaacaagtct tctattaaat gctgtaatct ttgtgacttt ctatctaatg tatcaatata
     7741 caatttacga ttttcgtctg ttatactttc atcttttaat aaatctatat aagtgattat
     7801 agatgtaaga ggtgttttta ggtcatgtga tacatttgat ataagctctg ttttcatctt
     7861 ttgactctta acctcttcat caacagcttt tttaaatcca gtttgaatat ttcctagttc
     7921 ttcttttata ggattaaaaa atcctaagtc cttctccata tttacttcta agtttccatc
     7981 tgaaattttc tttgtaacat ctaaaagttg attatattca catattgtct ttttagagta
     8041 atctgacaat atcttaaata gtactgctga atatatcaat gcgagtatta ttccaaagaa
     8101 ccacatacta cacattataa ctataattaa taaatttatc cccaaaatta taactatagt
     8161 tttgtttggc ttttctttta gatttattgt tctaatttga ttaataactg tttttcctgc
     8221 tttttttaac catacaaaaa atattcctgt cacagtattt tttataaaat attctcttat
     8281 tccaacattt aaaatatgtt ttaaaagtgt tataaataca aatattattg agaatattac
     8341 aaaccaataa gttaaattca ttaaacttac caatatacta gccgattcag cacttatatc
     8401 attgtttatc agatactcta cataatttcc attcaaagta tcacttatta taccagtagg
     8461 atataaatct acaatccctt gtataatagc aagaataata aaaagtatct ctaatggtat
     8521 tcttgataat gtatcaaatc ctagtaattc cttagatttt ctgtatggga atactagagc
     8581 tacaatcaca actatagcta atataatttt taaaaccatt ctagaaattt catagtaaga
     8641 gttgttttct acattgttta tatacgaact tatattatca ttactataca tatttccata
     8701 actatactct ctactatcgc ctttacttat tactttaggt acagcatata ttagtgtcat
     8761 gttttttatt ggagataact tatatgaaat tgtatttcca cctaaatcta tatccattaa
     8821 atcttctctt gatttagata acaacatttg attaataact ttttgattta ctccataaga
     8881 atccacaaca tttacttttc catctttatc aaaattcatt acaacataga atctatagtt
     8941 ttcctttata tctgtaatct gtttttgtct ttcctcatca ttcatttctg tattttttga
     9001 ctggttttca tcagaatctt cctttgaaac atcattcttt gtcgtatcaa gtgctcctaa
     9061 ttctttatta tcattaaaaa attcaaattt actttcttta tcaactaaat aatattttaa
     9121 attttttctt gactctttaa tgaaagactc ccaactttca aagttttcat taaaatctgc
     9181 attaatacta tcatctgtat aataagtttc tgaaccatca taagtatctt tttctttact
     9241 tacatttttt atatagttca tagtttcttc attgataaat agctttgatg gttgtatcat
     9301 cttaccttct tgttcttcct tcatattatg atataaaaca taacttcctt tatatatact
     9361 atctacaaag ctttcctgtt caaatggatt ctttgctcca cctttagcac ctgcttgtat
     9421 agtagaatat tgactaatca tacctaatga tgctgataac ataagtataa aaactataat
     9481 agtaactata tatttatctt taaatttttt caattttgta tccaacaccc cacaccacct
     9541 ttaaatattt aggatttttt gggtcaatct ctatcttttc ccttatattc ctcacatgta
     9601 ccataactgt atctgtattt acagcatttt cattccaaac tctttcatat atttcatcag
     9661 cagaaaaaac tcttcctgca tttctcataa gtaaacttag tattttaaat tctataggag
     9721 ttgtttttat atactttcca tcaatactaa cttcttttgt gttttcattt aattctaatc
     9781 cacctattgc aaacacacta tcatcacttt ctaccttttg ctctttattt ccaaccatgt
     9841 ttagatattt agtgtacctt ctaagttgtg aattaactct agccaatagt tctaatggct
     9901 taaaaggttt tgttacatag tcgtctgctc ctatattaag ccccattatt ttgtcaactt
     9961 cttctgattt tgcagatagc attattactg ggaaattata gttttctcta agcttcattg
    10021 taagtgttat accatccatt ttaggcatca ttatatcaac tatagctaaa tgtatttctt
    10081 ggttttctat aaccttaaga ccttctaatc cattgtatcc cttaaaaaca ttatatcctt
    10141 gatttaatag atatatttct atagcatctg ctatttcctt ttcatcttct accaccaaaa
    10201 tattatatga attcattcta atctccttta tattaattta taaattttct ttacatatat
    10261 gattatacaa tatgatagaa gtttttccat ttatatatat atttatatct tttgttctgg
    10321 ttatccgtat ttaatgtcca tcttagatac ctccttctag tttaaattta atat
//
LOCUS       DAEMAE010000130        10213 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEMAE010000130 DAEMAE010000000
VERSION     DAEMAE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.132"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            321..1751
                     /locus_tag="KPH93_002514"
     CDS             321..1751
                     /locus_tag="KPH93_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1689321.1"
                     /translation="MSSQESKPKGNLISNFRDDMRNPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNRRNA"
     gene            1821..2957
                     /locus_tag="KPH93_002515"
     CDS             1821..2957
                     /locus_tag="KPH93_002515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG1689322.1"
                     /translation="MKIHIQRGLLKMIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKE
                     IIKLASNENPLGPSPKAMETMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVAN
                     GADNVITLIGEAFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGIL
                     DRITDKTKLICVCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVD
                     GLKYVRENYNVIVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGA
                     TAALDDEEFIKETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDL
                     KKRGFVIRPSATHARVSIGTMEENKKFVAVLKEILKESQVLDNR"
     gene            2965..3201
                     /locus_tag="KPH93_002516"
     CDS             2965..3201
                     /locus_tag="KPH93_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1689323.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3218..4267
                     /gene="vorB"
                     /locus_tag="KPH93_002517"
     CDS             3218..4267
                     /gene="vorB"
                     /locus_tag="KPH93_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG1689324.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDIAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREDDLGWTYDGSNRDHAKVADNQKPTFCMEKRI
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVNKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4269..5009
                     /locus_tag="KPH93_002518"
     CDS             4269..5009
                     /locus_tag="KPH93_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG1689325.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKATGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5012..5551
                     /locus_tag="KPH93_002519"
     CDS             5012..5551
                     /locus_tag="KPH93_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG1689326.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDILVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5675..7774
                     /locus_tag="KPH93_002520"
     CDS             5675..7774
                     /locus_tag="KPH93_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG1689327.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTNLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDNYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPIIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPTNSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCQAVSEARKQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSKPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8041..8556)
                     /locus_tag="KPH93_002521"
     CDS             complement(8041..8556)
                     /locus_tag="KPH93_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG1689328.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDADFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8648..9859)
                     /locus_tag="KPH93_002522"
     CDS             complement(8648..9859)
                     /locus_tag="KPH93_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG1689329.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSASNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3866 a         1253 c         1867 g         3227 t
ORIGIN      
        1 aataaaatta taaaaactta aaatcactag aaatatgata aaacaattta aaatacgtat
       61 tttaattaca tttgaaaaca agaaaataat aaaaaataaa tctaaattaa gcctaaatag
      121 ctttttagtt aattttaata gtaaaaaata aaatttaaaa agtgtttgac aattatgaat
      181 tttgagaata taattaaatt aaaattatac acatataaaa atatactcct gaatcgagaa
      241 tgagatattt aagattcaaa atatatatta ttatacgtga ataataaaaa gtaataaaat
      301 tttataaggg aggggtagat gtgagtagtc aagaaagcaa accaaaagga aatcttatat
      361 caaattttag agatgatatg agaaacccta agaaaagagc aatatacaca tattcaagtt
      421 ctataaactt agcgtacaat tttataggtt actttgtatc aactgttaca aatatctttt
      481 taactgatgt ggcaaaactt ggtgtaatta tggcaggtgg tatgcaaatt atacaatctt
      541 tcataaaaat tgctgtagct cctataacag gtacagtatt tgacaaacaa ccattcaaaa
      601 aaggaaagta ttatccatgg cttagatatg caccagcagc attagcatta acatatggaa
      661 tatttttcat agttccatta atgaaaataa gtccagcatt gcttttacct ttgatgacag
      721 ctttattatt aatagcatct tgtgctcagc aagtagtttt tactataatt tatgctattt
      781 accctgtagt tgctaagaca cctagagata gagttatagg ctcaactact acgaatatat
      841 tcaaagaagt tggtaaattc ttagtaggtt tttcatatcc gttattatta gttttatttg
      901 cagatgtatt aggtggagaa ggaatgggtt atttaggtac ctatttattc tttgcagcag
      961 gatgcctttt agtattttgg ttttcttcaa cagagataat aaaatctgga gcagaagaag
     1021 aagttcttca aaaagaagag aagccaaaga ttaaagcaag tgaaatggca aaagctttat
     1081 tcactaatgt accacttatg atggcatttt gtttagaatt tcttatatgt ataagatcta
     1141 taggattagg acctttagct ccatattatt ttaaatatgt aatagaagac gaacgtggat
     1201 tagcagtatt tttatcaatc atgcctttgg taagtgtagc attcatgttt ttcgcaccag
     1261 tgtttataaa aatctgtaga gagcaaaaac tagcttcaat aatttctttt tctatatgtg
     1321 cgttatgcca cttattggtt gcatggactc cttgggggaa aactacttta ggagtaacta
     1381 tgttattggc aattggtgga ggtttttcaa atgtagtatc aataataaat cttaatttct
     1441 ttgccggttc ttgtgattat gggcattgga aatcaggaaa agacttacct ggtttaagta
     1501 tgtcactata tccagttgca atacaagtag gagtattact tgcaactact ataagaacag
     1561 ttttaatgaa ctcaatggga tatcaagctg atatggtagt cacagaagca gttaaatcag
     1621 gttttataaa tatgatttct tattctatgg caataccttt aataatagcc gttgttatag
     1681 caatactata tccagtaagt gacaagaaat taaaccaaat aagagaagag ttaaatcgaa
     1741 gaaatgctta gttaaaagaa agttaagtag atggttaatt taagctatta gagtaagtag
     1801 acgcaatagc gtttatataa atgaaaatac atatacagag ggggctttta aaaatgatag
     1861 aaaaattatt gagaaatact gtaaaagaat tacatcaata tgttccaggt gaaccaatag
     1921 aaaaagttaa ggaaaagtat ggtgtaaaag aaataataaa attagcttca aatgaaaatc
     1981 ctctaggacc atcaccaaaa gcaatggaaa ctatgattga aatgttaaaa caaggtcaac
     2041 tttatccaga gccagaagct aacgaactta gaagaaaatt agctgaaaag ctagatttaa
     2101 aaccagaaaa ttttattgtt gctaatggtg cagataacgt tatcactcta ataggtgagg
     2161 cttttataaa tagaggagat gaagttatat attgtaaccc aacatttcca tcatatagaa
     2221 ctgcaactat aaaaaatgaa gggataccag tagaagtacc tttaacagaa gattataagt
     2281 atgatttaca aggtatatta gataggatta cagataaaac aaaattaata tgtgtatgta
     2341 accctaataa tccaacagga acaattgtag atgataaaga gttagaagaa tttttaaaga
     2401 aagtccctga aaatattata actatattgg atgaagcata tatagaattt ttaacagttc
     2461 ctaattatgt ggatggactt aagtatgtaa gggaaaatta caacgttata gtaacaagaa
     2521 ctttctctaa aatatatggt cttgcagggc ttagagtagg atatggtatt gctaaagatg
     2581 agataataag aactttattt acagtaaaag agcctttctc tgcaaatcgt gttgctataa
     2641 gtggagcaac agcagcactt gatgatgaag agtttataaa agaaacatat gaattaaata
     2701 gagttggaat ggcttacttt aaagaagagt ttacaaaaat gggattcgat gttgtagatt
     2761 cacaatcaaa tttcctttat gtagatatga aaacagatat acctaagtta tttgaagact
     2821 tgaaaaaaag agggtttgta ataagaccaa gtgctacaca tgcacgtgta agtattggaa
     2881 ctatggaaga aaataagaaa tttgtagcag ttttaaagga aatattaaag gagagccaag
     2941 tattggataa tagatagaaa tattatgaaa cggggtgtag atatgaactt aaaaataaac
     3001 gcagagagat gtaaaagttg tgaatactgc gttatatctt gtaaaaaagg agcattaaaa
     3061 atatcaagta agataaataa agaaggttat gctcatgtag aagttgatga aagtaaatgc
     3121 atactttgtg gaatttgcta tcaagtgtgt ccagataatg tttttgaaat aattaaagaa
     3181 gaagcatgtc aagaggctta gaaggagttg ggataatatg aaaaagatgt ggaaggggaa
     3241 ccacgccatt gcagaagcag ccctaagagg aggttgtgaa ttttatgcag gatatccaat
     3301 tacaccacaa acagaggtaa tggaattttt atcacataga atgtctgaac ttggaagaac
     3361 ttttatacaa tccgaaaatg agatggctgc tatatatatg gtatatggag catatgcaag
     3421 tggaatgagg tctatgacga gttcttcagg accaggaata tctttaaaac aagaagggat
     3481 ttcttactta tgtgcaaatc attacccttg tgtaatcgta aatgtacaga gatgggggcc
     3541 tggtcttggt tcattggact cagcacagac agactatcta agagatacta gagggggagg
     3601 aaatggagat tatcacttga tagtttatgc accaaacagt attcaagaga ctgtagattt
     3661 gatgtataac tcatttgata tagcggagaa gtacagagta cctgttgaaa ttttgacaga
     3721 agcagcttta ggtcaaatga tggaaccagt agaatttcca gagtttaaga aaagagaaga
     3781 cgatttaggt tggacttatg atggaagtaa tagagaccat gcaaaggttg cagataacca
     3841 aaagccaact ttttgtatgg agaaacgtat aagaataact gaaaatgaac aacaatggga
     3901 agattatcaa gtagaagatg cagaatatgt gtttgtggcg ttcggcatac catcaagaac
     3961 aactatgaat gctgttagaa gacttagaga acaaggtgaa aaagttggaa taataagacc
     4021 aataacagtg tggcctttcc catataaagc atttgaaaag gtaaacaagg atgtaaaagg
     4081 atttatttca gtagaatcaa cagatacagg tcagttagta gaagatgttg ctttagcctc
     4141 aaagaaagta tgtaaagaaa atgtgcctgt gtatggtcta tttagtggaa atcatatacc
     4201 aaaaacattg caggtaatgg acacttatag caaaataaaa tctggcgaaa tcaaggaggt
     4261 gttctaatat ggctacagaa aaattaacac ctagcgttgt ttcaatacca aacaagtttt
     4321 gcccaggttg tggtcatgga atagtaaatc gtataatagc agaggtaata gaagaacatg
     4381 gatatgaaaa aaatcatgta ttaacattag gagttggttg tgtctgcaat atgaatttta
     4441 gctggaatgg tgataagatg caaactgcgc atgggagagc ttcatctaca gctataggag
     4501 ttaaagtagc acttccagat acattagtga tgacttacca aggtgatgga gatgcttatg
     4561 ttataggtct ttctgaaact ttaaatacag catatagaaa tcataatgtg actgtatttg
     4621 taataaacaa taataatttt gcaatgactg gtggacaaat gtcatggacc actatgccgg
     4681 gtcaagtcac aacaacaagt gtaaatggaa gagatataaa ggctactggt agtccactga
     4741 aagttcctga gatggtggca aacttctttg atgtagcata tgtagcaaga ggttcagttc
     4801 atagcccaaa ggaaataatt aatctaaaaa aatatataaa aaatgcaata gaagctcaat
     4861 taaatggaga ggggtattcc ttagttgaaa tattagcacc ttgcccaact aactggggtg
     4921 tatcactaga gaaatctatc aaatggatgg aagaagaaat agtgccatat tatgctcttg
     4981 gcgaatttaa gcaaagggat ggtgaatagt tatggaaaaa gagctagtat ttgcagggtt
     5041 tggtggtcaa ggagttttaa ctcttggtct gattgtagct gaatcagcac ttgaattggg
     5101 gaaacaagta acttggatgc ctgcatatgg tccaactatg agaggtggaa aagcctatag
     5161 tgtagtaaaa ttttctgatg agagtatagg tggacctgat atggaagaaa tagatatttt
     5221 agtagcaatg aataaaccat ctttagacta tataaattta gttaaagaag gtggaacagt
     5281 agttattaat acatctgcaa tagatgagaa tgtaacatta agagaagata taagtgtagt
     5341 taaaataaat tgtcaggaat tagctcaaaa agtaaacaat ccaaaagcag caaatatagt
     5401 agtacttgga gcattaattg ctaaaacggg gttatttgaa aaagaattag ctttaaaaac
     5461 tatgtgtgat ttctttgagg aaaaaggaaa aggaaagttt aatgttcaaa atgaagcagc
     5521 attcatggaa ggctataata acgccatttg ataaattacc cttatcttaa gtgatttatc
     5581 tcaagattta ataatcttta gtgccaagag aaacttcatc ttgagataaa attcacttaa
     5641 aatcacaact ataaacatat aggagcgtga atttatgagt ttggaaaaac ttattagacc
     5701 aaaaagtatt gctatagtag gagtgactga taaacttggt tttggaagaa gtgcagcact
     5761 tagtatagta aaaagtaagg agacagatag agtatattat gttaatccca aaagagaaga
     5821 attatttgga agaaaatgtt acaagacgat acaagaagtt ccagaagtag tagactgtgt
     5881 agtagtatgt acacctagaa atgtagttcc atctgtatta aaagattctg gagagttagg
     5941 aacaaaggca gcggtggtat atgctagtgg atttgcagag gaaggtacag aagaaggtac
     6001 aaatttagaa aatcaactta tagaaatatc aaatacatac gatatgaaaa tattgggtcc
     6061 aaactgcatg ggattgctaa attgtataga caaagttaat ctgtgggcag gaggttctaa
     6121 atgggattta gatactaaaa aacctggtat aggaattgta ggtcaaagtg gatttataac
     6181 tgctgaaatt gtctcaagtg attattttaa tatatcttat ggtttttcaa caggaaatgg
     6241 aaatatagtt acactagaag atgcagtaga tttccttgta gatgataatt atgcatcagt
     6301 aatagcaatt taccttgaag gtttaaaaaa tcctcaaaaa tttatagatg ctttaaaaag
     6361 agcagcacaa aaaagaaagc caataataat attgaaatca ggaaggtcag aaaaaggtgc
     6421 tatttctgct gcttctcata caggaaattt agctggttcg agtaaagctt ttgaaagtat
     6481 ttttgaaaaa tatggagtaa tttctgtgga aaatttagaa cagtttatgt gcttagcaca
     6541 agcttttagt gttctagatg gtaatttacc tacaaattct aattttgcag caataaactt
     6601 ctcaggagga gaaaatacta tttgtgcaga tttagcagaa gaaaatggtg tagaacttgc
     6661 agaaatatct tctgaaacta aagaagaaat gaaaaaatat cttccaggat ttgcaacacc
     6721 taaaaatcca ctagatgcta caacagcatt gttccacgaa aaggatatga tagtaggtct
     6781 attacatact tttgaaaagg atgatagtgt tggatttact atggtaggtg caaatataag
     6841 agatgaagaa aatgaaatgc atgaaacact ttgtcaagct gtaagtgaag ctagaaaaca
     6901 aggattgaaa aaacctgtgt ttgcagttcc tacacttgaa ggaaatagat atttagatta
     6961 taggactaga ttagaagata atcaagtgcc tataatgtca tcagttggaa ctacatttac
     7021 ttgttttaat aaaatggcta agtttataga ttatgattat tctaagagaa ctttggaatt
     7081 taaagctgtt aagaaaagag agtcaaataa tgtagtagca ttatcagaat tagatagtaa
     7141 gattgagatg aaaaaatatg gagtaccagt tccagggcaa ggtaatgcaa aaagtattga
     7201 tgaattagac gaaatcttaa aatatattaa atatcctgtt gttttaaaaa taaattcttc
     7261 tgaaatatta cacaaatctg atgttggagg agttaaaata ggaattaaga atagagatga
     7321 ggcagtagat gcatataatg agattttaac taatgtaaaa aaagcaaaac cagatgctaa
     7381 tattgatggt atactagttc aagaaatggt agaaagtggt atagagatta ttattggtat
     7441 aactaatgat gaccaatttg gaccaatgtt attagtcggt ttaggaggag tatttgtaga
     7501 ggtatttaaa gatactacat tatatccatt acctataaat catgatgaag ctattatgat
     7561 gcttaaaaaa ctaaaatcat ttaagctttt aaatggctat agaggaagta agccttgcga
     7621 tattgatgct cttgcagata tgatggtcaa attaggaaaa tatgcttatg aaaataagga
     7681 tgaagtcaag gaaatagatt taaatccagt gtttgtatat cctaaaggaa aaggcgtatg
     7741 tgcagtagat gccttaatag ttaaatataa atagtttata agttttggta ttcaaccata
     7801 attaaagcac cgactgaaaa tcacattttg taagttagat agtgtgattt tcggtcaccc
     7861 caaatttaat ttaaaaatat tttaaagtga aagtgtagtt ttaagtttca ttgtgaaata
     7921 gtactgtata aacaataaaa tactatgtat aaaatgaata ataaaaggtg ttttaatgat
     7981 aagaatcaat cttgattttc attaaaacac cttctttatt atattaaaaa tttttaagcg
     8041 ttatattgtc acaggtgggt ttgttggagc tgcatatgta cggttagcaa attcttggtc
     8101 aacaaagaat aaaccaccct ctccagcaag tttaacacgt tttatattgt cttcacaatt
     8161 tgtttcttct tctgcttgtt cagatataaa ccaaagtaag aattgagaag ttctaaagtc
     8221 tgattcagag ttggctacag ctgctaagtt gtttattaaa gctgtaacct ttttttcatg
     8281 agctaacgta cgctcaagaa tatccattgc agaagtaaag tctgcatctg gcattgggat
     8341 agcatcaagc tgaacctttc ctcctacatt atgtaagtat ttgtaaaaaa acatagcgtg
     8401 gtctgtctct tctttatatt gaacataaaa ccaattagaa aatcctttaa gtccttctgc
     8461 ttcaaggtaa gaagacatag aaagataaag atacgcagaa tatagttcat ggttaatctg
     8521 ctcattaagt aatttttcca ttttagctga aatcattttt atacctccaa attttaaatg
     8581 ctcttataaa atataataat attaaatgga aaaatattca ataaaagtgt ttaaatattt
     8641 tacatattta ataaaaattt atactaccat taaaaaaatt ataacttaca atggctatag
     8701 agccgattaa tggtgcattt gctccaaaat ttgaatgtct tacacttatt tttttatatt
     8761 tagaaaaact tgctgaagca gtcaattttt ttaataatgt attttctata aatgtaccag
     8821 gtatattaaa gtcatagcct attattatag aactacaatc aatcaatgta agtgtattaa
     8881 ctaatgcata tgatacgtat gtacaaaatt catcaagcaa aaacatggca agactatctt
     8941 tattatttgc ctcatctatt aatttgacta aagaaaaatc tttacagtta attaatgggg
     9001 agttaggata aatatgactc aatgattcaa ttcttttaat tagattactg gcacttgtat
     9061 aataatctaa acatccattg ttaccacaac tgcacaatgg tccactaaaa ttaattgatg
     9121 tatgtcctat ttctccgctt tgtcctaggt tgccagtatg aagcttattt tctagaacta
     9181 gaccagctcc gataccattc attatgtgta ggtatatata atttgatata tttttaccta
     9241 aaccatacat tttttctgct aatgcacccg aattagcatc gtttatcaaa aatattggca
     9301 aatcagacat ttcttttata aaatctacta tatttaggtt tgatacattt ccaaagtctg
     9361 ggggatttag tatggtacca tttatatcat ctactggacc tattgaagaa ataccaattc
     9421 caattatatc tctcttgttg attttcatta ttttagtaaa cattctcttt attatatctt
     9481 ttaaaacatc atttgataga catttagggt aagtttcaga aagttgtttt acaatattac
     9541 cttttaaatc tgaaataatt accttacaga tatttcttct tattaggatt ccacaaatac
     9601 aaggcgatat attagaaata tctaataaaa taggtttacg acctgatgaa tgtgcattta
     9661 aagttgatga ctctatttcc ctaatcatgt tttcattaat aagttcagta actagatttg
     9721 ataaagtcat tttagtaagt cctgtagact tagctaaatc aactctggac ataggtgtat
     9781 ttgtagaaag taatttcaat attattaact tatttttaat ttttacatct ttgatgttag
     9841 aaccttgttt gttactcatc gtaaatctcc ttaaataaag ttaagtgttt tatttatttt
     9901 caagtatact atcaccttat aatagagtaa cattttttac ttgaaaatat cattaattag
     9961 aatatatcac attatatatc ataaaacata taaaaaaatg ttagaattaa attaaaagtg
    10021 aaaagtagat tgatatataa atagtaaaca cttaattaaa aaaagataat gatagcaata
    10081 attattgtta ccaagaatct actacttcaa gtgttacctt aggtatttaa gttgataatg
    10141 gtttgcacat aataaacttg atgtaatata tcttcttaat aatatcgata aaagatttac
    10201 ttaaaaataa tat
//
LOCUS       DAEMAE010000131        10070 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEMAE010000131 DAEMAE010000000
VERSION     DAEMAE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.133"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..1128
                     /locus_tag="KPH93_002523"
     CDS             88..1128
                     /locus_tag="KPH93_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG1689330.1"
                     /translation="MYLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1267..1719
                     /gene="tsaE"
                     /locus_tag="KPH93_002524"
     CDS             1267..1719
                     /gene="tsaE"
                     /locus_tag="KPH93_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG1689331.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELKYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1719..2435
                     /gene="tsaB"
                     /locus_tag="KPH93_002525"
     CDS             1719..2435
                     /gene="tsaB"
                     /locus_tag="KPH93_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG1689332.1"
                     /translation="MKILGMDTSSMAASVAVVEDNNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVEEIVSTNEEFII
                     VGEAVYKYKDKLENIENIKIPSPANNVSNAGSLCSLALNKYSKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2425..2901
                     /gene="rimI"
                     /locus_tag="KPH93_002526"
     CDS             2425..2901
                     /gene="rimI"
                     /locus_tag="KPH93_002526"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG1689333.1"
                     /translation="MEDNIKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2907..3923
                     /gene="tsaD"
                     /locus_tag="KPH93_002527"
     CDS             2907..3923
                     /gene="tsaD"
                     /locus_tag="KPH93_002527"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG1689334.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4220..6928
                     /gene="hpdB"
                     /locus_tag="KPH93_002528"
     CDS             4220..6928
                     /gene="hpdB"
                     /locus_tag="KPH93_002528"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG1689335.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIVHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKIPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSVINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6932..7189
                     /gene="hpdC"
                     /locus_tag="KPH93_002529"
     CDS             6932..7189
                     /gene="hpdC"
                     /locus_tag="KPH93_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG1689336.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7182..8132
                     /gene="hpdA"
                     /locus_tag="KPH93_002530"
     CDS             7182..8132
                     /gene="hpdA"
                     /locus_tag="KPH93_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG1689337.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDMFLDNGIACYVGHETAF"
     gene            8440..9378
                     /locus_tag="KPH93_002531"
     CDS             8440..9378
                     /locus_tag="KPH93_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1689338.1"
                     /translation="MDKSRSNLIIKNKDKKVRSGWIIIAVMATFYILQYYSSTLLMEI
                     LRKILIARGDINLKTNYFSEYANWLNDFGLPIVFQILTDALMITIPIIVWRFIMKNPI
                     SEMGLHSYKIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVISWVPKISPLTVSWILV
                     FILIAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIIV
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSNK"
     gene            complement(9500..>10070)
                     /locus_tag="KPH93_002532"
     CDS             complement(9500..>10070)
                     /locus_tag="KPH93_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1689339.1"
                     /translation="LYPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQPNYSGIIKF
                     IFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKLGVIILGICWSLWHLPLIFTLY
                     TPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCAIIHILNNSAFLITNSKESTIT
                     YHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3955 a         1040 c         1873 g         3202 t
ORIGIN      
        1 aaaataaagt tattattaaa aaactataaa gattaataat caaactttta ataataacta
       61 atttaataat gataggggtg tatattcatg tatttgtcta aaaaaataaa aaaattattt
      121 ttagatgata aagggtatta ttttaatagt aattggaaag atattcatat tggtatttat
      181 gatacaaaat taggagattc tttaaagtat atcactaatg aatatattga aaatatggaa
      241 agggtaaaat acgattatcg tgaattaaaa aatatagaaa atcaaactga tggtatgtgt
      301 attttagcaa taaggcagaa ttacaaggca ttagagtatg taaaaaatca aaccgaagaa
      361 ttatgtttag aagcaataaa gtataattat aaagcacttg aatatgtaaa agagcaaaca
      421 gaatatctgt gtcttgaagc tataaaaaag gattgtaatg cacttaagta tgtacgaaat
      481 aaaacagaag ggcttattat aaaagcaata agtcattctt ccaatataga tgttgtaagt
      541 atactaaagg ccttagagac tcaaacaaga agaatatgtt tagaagcaat taaaaaagat
      601 ggcaggtgtc ttgcatacgt tagagaacag agtgaagaat tatgtataga ggccattaag
      661 caaaattata aagcactaaa atatgtaaaa aatcaaacgg aaaaaatgtg tatagaatcc
      721 gtaagacaaa atggtatggc attacaatat gtaaataaac aaactgataa aatatgtata
      781 gaagctgtca aacaagatgg aagaagtcta caatttgtca ataataagac agaagaaata
      841 tgtataaatg ctataagata tttaaataaa aaatataata ttaaagatgt actaagttac
      901 atagataagt atacagaaga tatatgtata gagattgtaa gacaaaatgg aaaaatgctt
      961 atgtatataa aaaatcaaac agaaaaaatg tgtattgaag cagtaaaaga gaactataag
     1021 tctcttaagt atgtaaaaga gcaatctgag agaatatgta aagaagcatt gaagcaaaac
     1081 cataaagcaa aagagtatgt aaaaattgct attgatgatt gtatataagg tgtagataca
     1141 gccatcaata gtgaaagata aaaaaagata aagacaagta ctttataata atctaggctt
     1201 atactataat taattaagca gtgatataaa ttcacatgac gattatatca aaatggagat
     1261 gaattgatgg caaaaatata tttagaaaat gaaaataaaa ctagagaaat aggatataaa
     1321 ttaggtaaac ttttaaaaga aggcagtgtt atctgtttag taggagattt aggagctggt
     1381 aaaactacaa tgacacaaag cttggcagat tcattaggaa tagaagatta tataacaagt
     1441 cctactttta ctataataaa tgaatatgaa ggaaaaatac ctttgtatca ttttgatgtt
     1501 tatagaatag gtagtagtga tgaaatgtat gatattggtt atgatgaata tgtaaattca
     1561 aatggaatct gtataataga atgggctaat ctaatagaag atatacttcc aaaagaatat
     1621 ttgaatatag aattgaaata taaagatgaa ggaagagaga tgatattaac tccaaaagga
     1681 gagttttata aagagatagt tgaggagttg attaaataat gaaaatacta ggtatggata
     1741 catcttctat ggcagcaagt gtagcagttg ttgaagataa taacttaata tgtgagttta
     1801 cagtaaacaa caaaaaaact cattcacaaa agcttatgcc aatgatagaa aatatgttga
     1861 gtatgagtga tttaagtata aaagatatgg atttattagc tgtatgtata ggcccaggtt
     1921 cgtttacagg acttagaata ggtatggcta cagtaaaagc tatggctcat gtaaataata
     1981 taccaataat agcagtaaat tctttagaat ctctagcaaa taacataaat ttctgtgata
     2041 gaaagatatg ttgtatatta gatgcgcaga aaaatcaagt ttattcagct aaatacaagt
     2101 ttgaaaatgg agagatgata gaattagatg gtgttgatgt tgtagagttt gatgctttag
     2161 tagaggaaat tgtatcaact aatgaagaat ttataatagt tggagaagct gtatacaaat
     2221 ataaagacaa attagagaat atagaaaata taaaaatacc ttcaccagct aataatgtat
     2281 ctaacgcagg aagtttatgc tctttagcgt taaataaata tagtaaaaat atagatgtac
     2341 atacttgtta tacgataaat cctatgtata tcagaaaatc tcaagctgaa gtacaatatg
     2401 atgaaaaaat gaagaggcta aataatggaa gataatataa aactaatagt agatgatata
     2461 aaaatagaag aaatgactac agaagatata gatgaagttt ttgaagtaga aaaaaattgt
     2521 tttgaagatt attggtcaaa agagtctttt agaaaagagt tgtctaatga agtagcaagg
     2581 tatatagtag ctaagttaaa tggaaaaatt gttggatatg tgggaatttg gcttatactt
     2641 gatgaaggtc atataaataa tgtggctgtt catagtgact atagagggaa aaaaataggg
     2701 gacaagttga taaaaggcat agttgattta tgtaaagata ataatattgc atctatgaca
     2761 ttagaagtta gagcatcaaa taaaatagca caaaatttat atagaaaata tggatttaaa
     2821 atgggtggaa taagaaaaga atactacagt gataataaag aagatgcaat tataatgtgg
     2881 aaccaattaa aagaggtgta gagtttatga gtgatataat aacattagct atagagagta
     2941 gttgtgacga aacagctgca tcagttttaa aaaatggtag agaagtactt tcaaacataa
     3001 tctctaccca aatagagaca cataaaaaat ttggtggtgt agttccagaa gtggcttcaa
     3061 gaaaacatgt ggaaaatatt gatatagttg ttcaagaagc tttagacaaa gcaaatatag
     3121 gttttaatga tatagaccat attgcagtta catatggacc tggactagta ggtgctttat
     3181 tggttggtct atcttatgct aaagcacttg catatacttt gaatattcca cttgtaggag
     3241 taaatcatat agaagggcat ctaagtgcaa actatataga gcataaggat ttaaaacctc
     3301 cttttataac attaatagta tctggaggtc atactcactt ggttgaagta aaagattatg
     3361 gcaagtatga aattttagga aaaacaagag atgatgcatc tggagaagca tttgataaaa
     3421 tttcaagagc aatgaattta ggttatccag gaggacctat tattgataat ctagccaaga
     3481 agggaaataa acatgctata gagtttccaa gagcctattt agaagaagat agttatgatt
     3541 ttagttttag tggattaaaa tcttctgtat tgaattatct aaatgggaaa agaatgaaga
     3601 atgaagaaat agtagttgaa gatgtagcag catcttttca agaggctgtg gtagaggttt
     3661 tatctactaa ggcactaaaa gcagtaaaag aaaagggata caatatcata actctttctg
     3721 gtggagttgc ttctaattcg gggcttagag ctaaaataac agagcttgct aaagacagtg
     3781 gtattactgt taagtatcct cctctaattt tatgcacaga taatgcagct atgatagggt
     3841 gtgcaggata ttataatttt ataaacggga aaacgcatga tatgagttta aatgcagttc
     3901 caaatttaaa gataaatcag tagagttaga taaatttgta aatataaaag tattgtttca
     3961 aaaaagtaag cagaagtgaa attaaatata ataaaaatca atatagtata tattaaaact
     4021 tgaagtaaaa ctatttataa tctttatatt ccaatgaatt gcaacacatt ttaggatata
     4081 caggttgtct ttggcttaga tttgcttcaa gtttaatttt ttctatagaa agattatttt
     4141 aaaaagtggg taatataccc ttttttttca ttcttaaaaa gttttatact aattatagaa
     4201 agattaagga ggggaagaaa tgagtcaaag taaagaagac aaaataagaa gtattttgga
     4261 agcaaaaaat atcaaatcaa attttcaaaa taaagaaaat ttaagtgaat ttaatgaaaa
     4321 aaaagcaagt aaaagagcag aagacttatt agatgtatat tataatacat tatctactgc
     4381 agatatggaa tttccatatt ggtataatag agaatataga aaatcagatg gtgatatacc
     4441 agtagttaga agagctaaag ctcttaaagc tgctttttca catatgacac caaatataat
     4501 acctggtgag aaaatagtaa tgcaaaagac acgccattat agaggttctt ttccaatgcc
     4561 atgggtaagt gaaagtttct ttgttgctca aggagaacaa atgagagaag aggcaaagaa
     4621 attagctagt aacacagctg atgaacttac aaaatttggg tctggtggag gaaatgttac
     4681 tgaaagtttt ggaaatgtcg tttcaatagc tggtaaattc ggaatgagaa aagaagaagt
     4741 acctgtactt gtaaaaatgg caaaagaatg ggttggaaag tctgttgaag atttaggttt
     4801 tcactatgaa aaaatgatgc cagattatga tttaaaagaa aatctaatga gtacacttat
     4861 atgtatgttt gattcaggat atacacttcc tcaaggtaga gaagttataa attattttta
     4921 tcctttaaat tatggactag atggaattat agaaatggca aaagaatgta aaaaggcagt
     4981 tgcaggaaat gcatctggag atgggcttat tggtatggac cgtctatatt tttatgaggc
     5041 tgttattcaa gttattgaag gattacaaac ttggatttta aattatgcaa aacatgcaaa
     5101 atatttagaa agtatagaga cagatttaga agctaaaaaa gaatactcag atttagtgga
     5161 gatattagaa catattgtac ataaacaacc tcgtacattt agggaagctc ttcagttgac
     5221 ttatacaatt catatagcat cagtaaatga ggatgctata tctggtatgt ctataggccg
     5281 ttttggtcaa attttatatc cttggtatga acaagatata gaaaaaggat taattacaaa
     5341 agaagaggtt atcgaattat tagagcttta tagaataaaa atcacttgta ttgactgttt
     5401 tgcttcagcg ggcgtaaatg gaggagtact atcaggaaat acatttaata ctttatctat
     5461 aggtggatta aaagaagatg gttctacagg agctaatgaa ctagaggagc tattattaga
     5521 agcaagtatg agatgtagaa ctcctcaacc tagtttaact atgttatatg atgaaaaaat
     5581 tccagaagat ttcttaatga aagctgctga atgtacaaag cttggttctg gttatccagc
     5641 ctgggtaaac aactcaaatg gaacaacatt tatgatgaag caatttgctg atgaaggtat
     5701 gactgttgaa gaagcgagag catttgcatt aggaggatgt ttggaaacgt ctccagggtg
     5761 ctggaaacag ttaactttaa acgggaagac atactctatt gcaggtggag caggtcaatc
     5821 tgctggctct ggagttcact ttatagctaa tcccaaaatt ttagaacttg ttcttatgaa
     5881 tggtaaagac catcgtatga atatacaagt atttgagcct cataataagc cattagatac
     5941 ttatgaagaa gtcatagaag tatttaaaga ttattataaa caagcaataa atgttttaga
     6001 aagagcaaat aacattgagt tagatatatg gagaaaattt gatacttctg taataaattc
     6061 actattaaag ccagactgtt tagacaaagg tcaacatata ggaaatatgg gatatcgtta
     6121 taatgcaaca ttaaacgtag aaacttgtgg tactgttact atggtcaatt catttgcagc
     6181 acttaagaaa cttgtatatg atgacaaagc atttacaata gaggaaatga aggatgccat
     6241 attaaataat tttggattta aagatgcatt agaagtagga aattattcta tggcagacca
     6301 agtaaaagta gataagactg gtaaatatga tgctatttat aaggcctgtc ttgatgcacc
     6361 taaatatgga aataatgatt tgtatgcaga taatattctt aaaaactatg aggtatggtt
     6421 atcaaaagta tgtgaagaag cacaatcttt atatgctaag aagatgtacc cttgccaaat
     6481 atctgtttct acacatggac cacagggagc tgctaccttg gctactccag atggtagact
     6541 aagtggaaca acatattcag atggttcagt ctctgcatat gcaggaacag ataaaaatgg
     6601 agtatatgca ttatttgaat ctgcaactat ttgggaccaa gcagtagttc aaaattctca
     6661 gatgaattta aaacttcatc caacaactat aaaagggcaa caaggaacta aaaaattatt
     6721 agatttaaca agaagttatt taagaaaagg tgggttccat attcaatata atgttgtaga
     6781 ttcagaaaca ttaaaagatg cacaaaaaaa tcctgacaat tatcgtcaat taatggtacg
     6841 tgttgctggg tttactcaat attggtgtga attaggtaaa ccaatacaag atgaagtaat
     6901 tgctagaaca gagtatgaag gggtgtaaaa tatgagaaag catagtgatt gtatgaattt
     6961 ttgtgcagta gacgcaacca aaggaatttg tagattatca aaacaaatga ttaatttaga
     7021 tgacgcagca tgtccagaga taaaagtaat gccaaaatgt aaaaattgta aaaattttgt
     7081 tgaagctaat gatgaaggta taggcaaatg tgttggtcta gagaaagaag actgggtata
     7141 ttcaacattg aatgcaatta cttgtgaagg acatgtgttt aatgagtagt caaaagcaat
     7201 tagaaggcat gattttcgat gtacaaagct tttcagttca tgatggtcca ggttgtagaa
     7261 caactgtatt tttaaatgga tgtccactga gttgtaaatg gtgtgcaaat ccagaaagtt
     7321 ggactgtaag accacatatg atgtttagtg aattatcttg tcaatatgaa aatggatgta
     7381 ctgtatgtca tggtaagtgt aaaaatggtg cattgagctt taatcttgat aataagccag
     7441 ttatagattg gaacatctgt aaggactgtg aaagttttga atgcgttaac tcatgttatt
     7501 ataatgcgtt taaattatgt gcaaaacctt atactgtaga tgaacttgtg caggtaatta
     7561 aacgtgattc taacaattgg agaagtaatg gaggagtaac atttagtgga ggtgagccac
     7621 tacttcagca tgaattttta catgaagtat tattaaagtg tcatgaggta aatatacaca
     7681 ctgccattga aacgagtgca tgtgtatcaa atgaagtttt taataaaata tttaaagata
     7741 ttgattttgc atttattgat ataaagcata tggatagaga aaaacacaaa gaacaaacag
     7801 gtgtgtataa tgatttaata ttagaaaata tatcaaacct tgcaaattct gattggaatg
     7861 gaagattagt acttagagta ccagttatct ctgggtttaa tgatagtgct gaaaatataa
     7921 gtgatattat atcttttatg cataaaaata atttggtaga aattaatcta ttgccatttc
     7981 acagattagg agaatcaaaa tggattcaat taggcaagga atatgagtat tctgataagg
     8041 gtgatgttga tgaaggtcat cttgaagaat tacaagatat gtttttggat aatggtatag
     8101 cttgttatgt tggtcatgag acagctttct aaaatcaaat acaagtttta attaaaaaag
     8161 gatgttacat taaaagctag agttacatcc ttttttatgt gggttttatg tagaaataat
     8221 taaataaagt tttatatatt gtttatattg atttatgttc ataaatattg attcaaattt
     8281 ataccttata tattttttat agtttaaatt acagttagag aatatattac gtttacttta
     8341 ttgaagcaga agttttgtgg taaaatacta ctatgaaaaa atttaataga atatatttta
     8401 taaataacta aaaataatag ttagtaggag gtcatctata tggacaaatc aaggagcaac
     8461 ttgattatta agaacaaaga caaaaaagta cgctcagggt ggattattat agctgttatg
     8521 gctacctttt atatactaca gtattattca agcactctac ttatggaaat actaagaaaa
     8581 attttgattg ctagaggtga cattaatcta aaaacaaact acttttcgga atatgctaat
     8641 tggttaaatg actttgggtt accgattgta tttcaaattc taacagatgc acttatgatt
     8701 actattccga taattgtatg gagatttatt atgaaaaatc ctattagtga aatgggatta
     8761 cattcttaca agattaagaa gaaagaagga tatgtaggaa tgttgttagg aatatttaac
     8821 tgtacagtaa tctttattat aacaataaca attggtggtg gtcatgttat ttcatgggta
     8881 ccaaaaattt cacctttaac agtttcttgg atacttgtct ttattttaat tgcttttgca
     8941 gaagaaattt taaatcgtgg tttttttatg gcagttttac gaagatgtaa aaatatttat
     9001 tttataatga ttgttccatc tgttattttt gggttgattc atatatggaa tcctgatgtt
     9061 acttttttat ctgtaattaa tattataatt gttgggattc ttttttcata tatgtttata
     9121 aaatcgtcga atatatggat gtgtataggt tatcattttg catggaatgt atttcagggg
     9181 attatatatg gtatgcctgt aagtggactt caagttccag ggattataac tacacaaatc
     9241 actcatggta atctattaaa tggaggaatg tttggtattg aaggtggaat tttgactact
     9301 tttgttaatc tacttagctt tatatttgta tggtattact atagagattc taagtatgat
     9361 tttgttagta ataagtaagt gtgaataatg ttaagaacta attaattata ataaatttta
     9421 ataatatatt ctaggcttaa agtattctgt aggattgttg gaaatttaca aaaatataaa
     9481 aataacttgg atattctatt tactccaagt tatttttata ttcttttgta aatagaaatg
     9541 gaacataaaa aattaaaaca accatagata ctattagtat atcatggtaa gtgatagttg
     9601 attctttaga atttgtgata agaaaagcac tattatttaa aatatgaatt atagcacaaa
     9661 accagatgtt cttagttttt atataaagat atcctaaaaa tattgaaatt ccaactatat
     9721 gtatcgaacg taatattaat cctaatattg gtgtttcagg tgtatataat gtaaatatta
     9781 gtggtaagtg ccacaaactc caacaaatac ctaatattat aactcctagt tttttaccaa
     9841 atttattaaa aaaaatattc tgtaaaaaac ctctccaggc atattcttca ctaaaaaaaa
     9901 tataaatagt agttaatgat gttattggag taaatataaa ttttatgata ccactataat
     9961 taggttgagc cccttgtagt attaatataa atgatgttgg aacaattaca gaaatcataa
    10021 agtaaataat taacaaagaa cctagtttaa tattttttac aggatataaa
//
LOCUS       DAEMAE010000132         9973 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEMAE010000132 DAEMAE010000000
VERSION     DAEMAE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.134"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(441..1598)
                     /locus_tag="KPH93_002533"
     CDS             complement(441..1598)
                     /locus_tag="KPH93_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG1689340.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKTKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1752..2321
                     /locus_tag="KPH93_002534"
     CDS             1752..2321
                     /locus_tag="KPH93_002534"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG1689341.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNGKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKEAHDAQLDYIVTETRIITPNN"
     gene            complement(2503..2919)
                     /locus_tag="KPH93_002535"
     CDS             complement(2503..2919)
                     /locus_tag="KPH93_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689342.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3145..4239)
                     /locus_tag="KPH93_002536"
     CDS             complement(3145..4239)
                     /locus_tag="KPH93_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689343.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGMFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(4662..5603)
                     /locus_tag="KPH93_002537"
     CDS             complement(4662..5603)
                     /locus_tag="KPH93_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG1689344.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            complement(5596..6423)
                     /locus_tag="KPH93_002538"
     CDS             complement(5596..6423)
                     /locus_tag="KPH93_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG1689345.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYILLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(6594..6977)
                     /locus_tag="KPH93_002539"
     CDS             complement(6594..6977)
                     /locus_tag="KPH93_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG1689346.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(6946..7233)
                     /locus_tag="KPH93_002540"
     CDS             complement(6946..7233)
                     /locus_tag="KPH93_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG1689347.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(7266..8423)
                     /gene="alr"
                     /locus_tag="KPH93_002541"
     CDS             complement(7266..8423)
                     /gene="alr"
                     /locus_tag="KPH93_002541"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG1689348.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPEEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTD
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(8438..9028)
                     /locus_tag="KPH93_002542"
     CDS             complement(8438..9028)
                     /locus_tag="KPH93_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689349.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(9049..9510)
                     /locus_tag="KPH93_002543"
     CDS             complement(9049..9510)
                     /locus_tag="KPH93_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG1689350.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(9525..9905)
                     /locus_tag="KPH93_002544"
     CDS             complement(9525..9905)
                     /locus_tag="KPH93_002544"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG1689351.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KVESIAGNFAAKEAISKSVGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         3196 a         1858 c         1058 g         3861 t
ORIGIN      
        1 atattgataa gtatgaaatt gtttaaccaa gtcaaaatga tatataaatt tatatttaaa
       61 tgtgtttaga gtttaattta ttttcaatgt ttaaaaaagt tattctatgc atctaattta
      121 attagtaaac tgaggttcta gttagataaa ttatattaca atgaatatat aatatgcagt
      181 ttgcgatatt ttaaaagtaa atgatataat atttggaaca actgggttgc aatgtgtttt
      241 taaaaaatat caaaataagg ctatgatttt taaaaccata gccctgtctg taaaaattta
      301 attatcactt aaaaaataaa tttttataaa aaaatacaca taaatatttc cagactccct
      361 catgaactaa aaagttcatt ttgtgacatc tcttttataa tgttctttat tttatagtat
      421 aagtttataa aaattaaatc ctatttatca atctgttctc taagccactt agttgctaca
      481 tctaactgtt tattttcatc tttctcttca atagtaggct ttataccttt ttcattgata
      541 taatctccat taggtgtaaa atattgagca gtagttagct tgtatcctgt tccgtccttc
      601 aatcttacaa ctgattgtac caacccttta ccaaaagttg tagtacctac agaaatgcct
      661 tctttattat caacgattgc agctgttaat atttcagcag cagaagcact ttctccatta
      721 gttaacacta ctatcggaag tcccaatttt tctttatcag atttcaaata ctcagtattt
      781 cccttattat cttttgtata tactatagtt ccttcaccaa ttaactcatc agcaacttct
      841 ttacatatat ctagcattcc accaggatta tttctaaggt ctattactaa tcccttgata
      901 ttttttgttt ttaattcttt taaagcttta tcaaagtcag tatatgtact tgatataaat
      961 tgggtaattt ctatatatcc taagtcatta tcaattactt tactttctat actcttctca
     1021 attatctggt ctgtttttac tttaaagttc aattgtttat cttctcttaa tatagtcaat
     1081 tcaactgttt tccctttttt acccttcatc atacgaacag cttcatctga atttttatat
     1141 gagacagatt ttccattgac ttttactaat ttatctcctg attttacacc tgctttttct
     1201 gctggagagt ctttcatagg aactactact aagccaccat cttttgatgc ccctatatac
     1261 atacctacac caacataaga accttcacta tcattaatta gattctccat ttcatcttta
     1321 gtataataac cagaatactt atcatttgtt cctagaaata gaccttttga tgctccattt
     1381 accaaatcag tatctgatac ttttttataa aaatcttgtt ttataataga ttctagtcct
     1441 aacaacttat catatttttt atagtcttca tagagttctt tcgatattac aactttattc
     1501 cccaatgtca actgaaatgc tgatgtaacc atagctgtta taattactaa aatcacccct
     1561 aaaaaaatag ctttcttctt tgaaatcaca agatacatct ccttttacat aaactattat
     1621 tattatatga ctagctgtta tttttattac atttacccaa atatattgct ttattacaat
     1681 aaattatact aaaatttata ttaaatacaa ttgctgtaca cattataaca tattaaggga
     1741 ggaattattt tatgaaaaaa gaatttagaa aaaaagttat tgaatcaaga aaaaataaga
     1801 gtgcaaactt cgtacttagt aattcaaatt taataactga aaaattactt caaatggaag
     1861 atataagaaa tgcaactaat ataatgcttt atctaaattt taacaatgaa gttcaaacag
     1921 ataatttaat caaaaaactt ttgtctctaa agaaaatagt atcaagtccc atcacaataa
     1981 aagaaaatca tacattaatc cctactcaaa taacagattt aaaagaggga ttgagaattg
     2041 gagcatatgg aataagagaa cctaacggaa aatcacctac aattgatatt aaggccttgg
     2101 atgttgtaat tgtaccagct gtagcatatg atatacattg ctatagatta ggatatggtg
     2161 gaggatttta tgatagattt ttagaaaact taagaaaaga tgctataact ataggcatag
     2221 catttgactt ccagatattc gattctattc caaaagaagc tcatgatgca caattagact
     2281 acatagttac tgaaactaga attatcactc caaataatta gaacattaag tacttccaga
     2341 ccttgtttaa tatagtaata aattctactg ttaatatctt actttataag cattttaagt
     2401 catggctttt acaacaataa gaaccataca caatttactt acattaataa aaaccatgac
     2461 tttattgtac aatatgcagt tatttaatta taaaatacct tcttatttcg tcgcatttaa
     2521 ttctattcta tctggaagtt cactcatatt tgtttcaaca actatgtatg gaaaagaatc
     2581 ttccttatct ttatacttta tcttgactct caaaacatcc tttccttcct caaccacact
     2641 ttctattttg tccatttcta taccatattt attatttcct ctagtcacta taatgtatat
     2701 tttgttatcg attttagccg ctaatgctct ttcttcattt gtatacttat ccatcacatc
     2761 tagtatcttt tctggtattg aatttctttg taatactgta taatctatag gtttagattt
     2821 ctctacaaac atacttggta tgaatataaa tccccctata acgaatacaa gagctataac
     2881 tccataaatt atacctttaa tattaaactt tacattcaat cttatcactc ctcccatata
     2941 ttctaaatat atgatttata aacttatctt agactctaaa ttattgatta gtatatattt
     3001 atccataatt ataaatatat gtaatgtaac taaaaaacat gtttttccta tttcataata
     3061 aacgagctaa aaaataaaaa gagctatatg aaaacatttt cattgttttc atatagctct
     3121 tgggtttaca atattaaaaa atatttattt gtcactgatt ccttcttgat atccagatgt
     3181 ttttgtaatc tgtctataaa gaacacaaac cagtattatg acgcccatat atccagtata
     3241 tggatataaa ataccaacca gcttatcaaa aggtaggaat ccacctataa gacctaatat
     3301 agctgctatt aaagttatta atttaaactt aggatgatta tcatctacaa atctattagt
     3361 tactgaccat aataaaggta cagctgttgt atatatacct aatataagaa ttattgagaa
     3421 taaaactcca attattggtg aaatcttatc tgctaagaat aatgatggta ttgcttttga
     3481 atataagttt cctatatcag aaagaatagc taagtgcatc attatagccg cagccataag
     3541 tgctactcca cctaatatac caccacaaat agcttctttt ttgttagcag cacttgctcc
     3601 taatccagtt aagaatgcca taacaacaat tgtgttataa gtagtatata ttacaccaga
     3661 tagataagca tttggaactg gtttagctat ctttacagtg ctaagaactt gtgcagcttc
     3721 tcctatatta cctatgtttt tagctaaact tataccacca actaaaagtg taaagattat
     3781 tattataggc cctattccac ctagtatctt tgataatcta tcaagaccta atgaaacagt
     3841 taataatgct actactgcca tacctactct acctacaaat ggatttaatc catagtattc
     3901 agttaaagtt gctcctgctc ctgaaatcat tactacgaaa acaccaaata agaataaagg
     3961 tccaaaccat tcaaagaaca ttcctaaata cttaccacaa taaacacgat aaattttgat
     4021 aggttcttta agttgaagct ctttaccttt tgacataact gaagcaccca tccatgagaa
     4081 taataccatt gaaattatcc caccaataat acctgcaaaa ccataaaaag agaagaattg
     4141 cattatttcc tgtcctgttg caaatccaga accgattact gttgctacat acgcacctgc
     4201 gaaactgatg actgttttaa catttacttt ttctgacata tctcagttac ctccccatgt
     4261 gaaatttttt cttctgctta aacaattcga taaactacct acaatttcct ttaacataaa
     4321 atacatgata actgttttat agctttttag tataataact taaaacttac aatctaattt
     4381 tataccctag tcctcctttc ataatttttt tagattcttt tatctaagta gtcattatat
     4441 ataaaattaa tttctctata aattatatat tagtcatatt tttctgaata gtcaatatat
     4501 atttaaatat tttctctatt tttgtaaaaa tttgaatatt ttattacatt tttgatatat
     4561 tgatatcata taaaaaaaag agagatataa attaatataa cactttatat ctctctttta
     4621 aatatattat attattaaca aacatgaata atttatatct cttacattct ttttgcaata
     4681 atagcttctt taacagtctt tactatgtca tcacaagtta attcatattt ttttaacaat
     4741 tcatttggag taccagattc tccaaatgta tctttaattc caactttctt tacaatggtt
     4801 ggacaagact ctcccactac ctcacttaca gcagaaccta atcccccaat tatgctgtgt
     4861 tcttctacag ttacaatagc attcgtttct tttgctgatt ctaatattaa atctttgtct
     4921 ataggtttta tcgaggacat gtttatgact ctagcattta taccttcttc ttgcaacttt
     4981 tcttgtgcta atagagcttc atttaccata attccacaag ctataattga tacatccttt
     5041 ccttctctta agactgtacc ttttcctatt tcaaatttat aatcttcatc aaataaaact
     5101 ggtactgaac ttctccctaa tctaacataa acaggtccat tatacttagc tatttcaaaa
     5161 ataatttttt ctgtttcaac tccatcagct ggaacaagaa ctgtcatatt tggtatagct
     5221 ctcattatag caatatcttc tacactttca tgagatgctc catcctctcc aacagtaaga
     5281 cctgcatgag tagcgcatat ttttacattt agctttggat aacaaactga atttcttata
     5341 atctcaaagg cccttcccgt agcaaacata gcaaaagtac ttgcaaaagg tattttacca
     5401 gcagttgata atccacaagc agcgcctatt aaattttgtt cagctatacc catattaaaa
     5461 aatctatctg gaaatgactt gtaaaaatca tgtgtttttg tagactttga taaatctgca
     5521 tctaatacta caacattgtc atttatttga cctaatttta ctaatgcttt accatatgct
     5581 tctctcgtcg ccattttact cattgtcagc acctcctaat tcaagcaagg ctttttctag
     5641 ctcctcatca cttggagctg tcccatgcca tcctgcttga ttttccataa aagatatccc
     5701 tttgcctttt atagtctttg caactatcat tgtaggtttt ttaactgttg atttagctat
     5761 atcaagagcc gcaaatattt ggtcaaaatc atgtccatct atctcaatta cattccaacc
     5821 aaaagcttta aatttatcaa ctattgaacc taaactcatt acactttcta tatctccatc
     5881 tatttgaagt ccattattat ctaaaaaagc aattaaatta tcaagtttat aatgtgcagc
     5941 actcatagca gcttcccaaa caattccttc ttgaacctca ccatcaccaa gcaaaatata
     6001 cacattccac ggagcattat ccaactttga tgccatagcc ataccacatg caactgaaaa
     6061 accttgacct aaagaaccag tagatatttc cacacctggc gtgcctttca tgtctggatg
     6121 accttgtaac atccttccta tcttccttaa tccattcagt tcttccttat caaaatagcc
     6181 tttttcagcc aatgtagcat ataatgctgg tgcagcatgt ccttttgata atacaaatct
     6241 atctctatcc tccattttag gatttgaaga atccacattc atttcatcaa agtataacgc
     6301 agtaagtatt tcaactactg ataatgaccc tcctggatgt ccagatttgg ctctatgtat
     6361 cattgaaact atatctcttc ttatgatacg cgcaatctca tttaatcctt tatggtctct
     6421 catgcaatta cctcccttga gttcgtaatt ttatatcaat tccatattat tatatcactt
     6481 ttatgctatt tttttgcgtt atttttaatt ttattaacat agtacattaa aattaaatcc
     6541 taagagatat ttaaaagaaa aaagggattg tctaataaaa gacaaatccc tctttaaaaa
     6601 gtaaacaatc ctaaacttat ttgaagtcca tcatctactt ttatcatcat attttcatct
     6661 agacaaccaa ttttttccct cagtctcttt ttatcaatag ttctaatttg ctctaaaaga
     6721 attactgagt ctttattaag cccatattca ttggaactta tctctacatg agtaggtaat
     6781 ttggctttat taatctgaga tgttatagct gatactataa ctgtaggact atatttatta
     6841 cctacatcat tttgtattat cagaacaggt ctaacgcctc cctgctctga accaacaact
     6901 ggacttaaat cagcgtaata taaatcacca cgctttattt cacaattagt actcaccttg
     6961 ggcaatcttc ccttcatttt tatcatcaga agatacttct tcaactgtca tacctaattc
     7021 agctagtgac agattaattt gacccatttc tctataaccc gttttcatag attctttaat
     7081 atgaattttc tttttctgag ttatatagaa ctgaaaagca gcttttgcaa aatcttctct
     7141 gttactgttc tccatctgaa ctatactatc aacttcagaa atcagagagt caggtaaagt
     7201 aacttctatt ttttctttac tcttatcgta cacaaaatcg cctcccagat tataatatca
     7261 tggttttatt ttagcaaata actgtttatt tgtacaagct cattattttc catataaaca
     7321 cggtcaactc ttcttgatat catacataac acttcatagt ttatagttcc taagtcttta
     7381 gctatacgct cagctgtaac ttcgccttct ccaaatagta taacctcatc tccaactttt
     7441 atgtctatat ctttgtcaat tctaaccatt atttgatcca tacatattct accaactaca
     7501 tcaaacactt ctcccttaat aagaaccttt ggatttttct ggattctagt aaatccatct
     7561 gcgtatccta ttggaactgt agctattgtt tctttatctg tagttgtgta ttttagtcca
     7621 taacttattc ctacacctgg ttcaacttgt tttatatgtc cgatttttga ttttaatttc
     7681 atggctggtc ttaattctaa tctatcttta aatacatcat cagatggata atgaccatat
     7741 aatattattc ctgctcttac catatttaat cttaaatcag ggcaatccat aatagctgca
     7801 ctgtttgata catgttttat agctattttt acgccagcct catctaattt atcagacata
     7861 aatttataat tattagcttg tttataagtg tactctttac ttacttcatc agctgtagca
     7921 aaatgagtaa acataccttc taaatctata tattctaatt tatttagttc tattatttcc
     7981 tgtactgact cctcattagg ttggaaacct attctagtca tccctgaatc tattttaaca
     8041 tgaacacagg ctttttctcc taaagatttt gcaatttcat ttatcttttg tgctgtttct
     8101 aaagaataaa ctgtcatagt tattttattt tttatagaat cttcaaaagc ttcttctgga
     8161 gtatatccca agttcaaaat aggaagtgtt atgccatttt gtctaagttc aattccttca
     8221 gcagttcttg ctactgctaa ataatctact ttttcttttt ctagcaattt tgcaacttct
     8281 actgcaccat gtccatatgc atcagctttt attactccac aaatcttaat atcttcttct
     8341 aataaatttt taatattatt taagttaaat cttaagttat ctagatttat ctctgcccat
     8401 gtaggcactg ttattttttg catgtcttta gtccccctta gtttctgtat tcaaaatctt
     8461 tgtattttac tgtaaatctt ggcactccct ctttatctaa cacttccatt ttttcaggat
     8521 tttttgtgtc agcatttaca tataatactt gcttgttaaa atatttatta tccccaggaa
     8581 taaaagtctc taaaaccaaa tgtcctttgg ataactttac tttcatctcc tcattttgaa
     8641 gatagttttt tataaagtct cctacaaaaa gatattgatt attttttcct gtattaggta
     8701 actccactac atcactaatc tttggatttt taactaatat tttatcacct tgatattcta
     8761 tacttttccc ttttaaatgt tttggggaaa ttacttctaa tttatagtta tcggtttttt
     8821 tgtaagtatg aattaaaaca taattgtgtg ggcttttatt tccaactact tctacttcag
     8881 ctttacaact gtaataattc atatcagaaa tctgcttttg aaaatctttg tatacttctt
     8941 cttttgtgga ctgtcgtttt tggcatccta ttacaattat aaccagtgcc aagaacataa
     9001 tacatactat ggtccacttt tttctcacat cacacctcct acttcagttt attcattgta
     9061 taaagctttt attatatcat atctacaaat tataccttta agtttgttgt catcatctac
     9121 tacaggaact ctgtttattg attttttaat cattacatta gctacatcat caaacttatc
     9181 atcttcatga acctttatta tatccttaga cataagttct ccaaccttat aagcagctac
     9241 ttgttttata tctttttcaa ctttttttaa gtcatctaag aaaatcaatc cttgtaatag
     9301 atttatatag agaggtgctt ctatatattt ttctttttta agaatatctg tttcagaaat
     9361 tattccaaca actctattct ctgaatctac aactggcaga cctcctattt tttctgcaat
     9421 tagcatatta gctacatctg ctatactatc atcttgtttt gctactatta catctgttgt
     9481 cattatttct tttgctgttt tatccatcat aatgtaatca ctccttagtc cttaattatg
     9541 gttatcgcat ttgctatagc ataatctttt gaatgtgata ttgaaacttt tatttctaaa
     9601 acattataat ctatgcacat ttttgctaga ttattataag tttttactat tggttttccc
     9661 atttcatttc tgagaacttc tatatcttta aagttaaata ctctaattcc tgtacctaca
     9721 gatttactta tagcttcctt agctgcgaaa ttacctgcta tactttctac tttaaaattc
     9781 ttagagttaa aatactctat ttctctatct gtaaatatct tctccaaaaa cctattattt
     9841 ttgtctacag cctttcttat tctatcaatt tcaattatat ctacgccaat atcaaatata
     9901 ttcattgttc ctccagaaaa tttataatac ttctttcaat aattaataat gaatttataa
     9961 atttactatc gat
//
LOCUS       DAEMAE010000133         9898 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEMAE010000133 DAEMAE010000000
VERSION     DAEMAE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.135"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..837
                     /locus_tag="KPH93_002545"
     CDS             163..837
                     /locus_tag="KPH93_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689352.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNLDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            861..1865
                     /locus_tag="KPH93_002546"
     CDS             861..1865
                     /locus_tag="KPH93_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1689353.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTISVITFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIKKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKVSDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYKQYTKFTITFKCDL
                     "
     gene            1977..3206
                     /locus_tag="KPH93_002547"
     CDS             1977..3206
                     /locus_tag="KPH93_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG1689354.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENRDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3214..3888
                     /locus_tag="KPH93_002548"
     CDS             3214..3888
                     /locus_tag="KPH93_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689355.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKVLKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3891..5123
                     /locus_tag="KPH93_002549"
     CDS             3891..5123
                     /locus_tag="KPH93_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689356.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5409..6674)
                     /locus_tag="KPH93_002550"
     CDS             complement(5409..6674)
                     /locus_tag="KPH93_002550"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG1689357.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMDAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASEIGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSTNPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6727..7875)
                     /locus_tag="KPH93_002551"
     CDS             complement(6727..7875)
                     /locus_tag="KPH93_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG1689358.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELSIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIVKNYKVPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7966..8511)
                     /locus_tag="KPH93_002552"
     CDS             complement(7966..8511)
                     /locus_tag="KPH93_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG1689359.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8734..9678)
                     /locus_tag="KPH93_002553"
     CDS             complement(8734..9678)
                     /locus_tag="KPH93_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG1689360.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMSKKLLKAGGVLGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3536 a         1516 c         1303 g         3543 t
ORIGIN      
        1 tatatttctt caaatttatt gcaattatgt ttaaaatata atttatcaag taaaatatag
       61 ttatattaat ataattattt taggtaataa ggtagaaata ttttaattta tcaagtagaa
      121 tatagttatg ttaatataac tattttgaat taggaggtaa gaatgtttaa aataatggta
      181 gttgaagatg acgtctcatt gaaaaatatc atagcaaagt gtcttactaa atggggacat
      241 gatgttcatc aaatagaaaa tcttgaaaat ataatagaag agttcaaaaa ctataaccca
      301 gaattagttt tattggacat aaaccttcca ttttatgatg gttttcattg gtgtaatgaa
      361 ataagaaaaa tttcaaaagt tcctataatt tttatttctt caagaaattc taatctggat
      421 gtaataatgg gtgtaaattt aggtgcagat gattatatac aaaaaccttt ttctgtagat
      481 gtcttagtag ctaaagttaa tgccctttta agaagaactt ataattttgt tgataataac
      541 tcaaatcaga ttattcacaa tggtgttact ttagatttat caactgcaac aataaactat
      601 gaagataata ctattgagct aacaaaaaat gaaatcaaaa ttctccatga gcttatgaaa
      661 tataaaggtc agatagtaag taggaataaa ctcatgaaaa aattatggga taatgattgg
      721 tttgtagatg ataatacatt aactgtcaat gtaaatagaa tacgctcaaa attaaatgaa
      781 attggtttag aagattttat agaaacaaaa agaggattgg gttatataat cagttaaaac
      841 aattctaagt aggtgaattt atgagcttta aagattttct caaagataaa ctaggttttt
      901 tagtatacaa ttttactttc ttaattttta caatatcagt tattacattt tctcctgtag
      961 atgtttttct gactgatact attttatata tattagttgt aaatgttgtg tttttatttt
     1021 tatatctatt tattagctac atcaagaaga acaaattttt agataaaata aaaaatgata
     1081 cattcaaaat taatatgaat gatatagaat tagctagatg taaaaatgaa gaaaaaatat
     1141 acttgaacat tattaaaaat catcaatatc aatgtaataa cataataaat aataatatta
     1201 agaaatttga agaaaacaaa gagattttaa atatgtgggt acacgatgta aagataccta
     1261 tagctataat aaaactcatt cttgaacaaa atgaaaattt aattgatgaa aaagtttcag
     1321 atgacataga tagtgaaata tttagaattg aaaactccgt tgaaaaaatt ttatatttat
     1381 caagactcga agattttcat aaggattttt taattcaaga ggtaaatatt gaaaaaatca
     1441 ttagaaatat tataaaaaaa tactcaaaat attttatttc aaaaaaaatt attttagact
     1501 taaataattt gaattttaag gttttatctg atgaaaaatg gctatacttc atattcgagc
     1561 aattattgag taattcccta aaatactcct cattaggaga taatatatct atctattgca
     1621 aaagaaccaa aaattattta caattaagag tggaagatac tggttgtggt ataaaaaagg
     1681 aagatttaaa tcgaatattc aataagggat ttacaggtaa taatggaaga aacaattcta
     1741 aatctacagg tcttggactt tatttagtaa aagaactttg tgataagctt ggtcataaaa
     1801 ttgatgtaga ttcagagtac aagcaatata caaaatttac tattacattc aaatgtgatt
     1861 tatagtatct tattattgat agtttatttt cattaactta tatttaactt cttatagtaa
     1921 ttctatatgt acatcccagt atataattaa gtagttttta taaaaaggag gaagacatgt
     1981 cttttctaaa aaaatttaaa aataaaagat ttagaaataa cagttttaaa agtaacaact
     2041 ttagaaatag cagttttgga agtagtggct tcaagtctaa aaataaaggt tttaaaaaca
     2101 atccctttaa aaataagaga ttcaaaatat atgcaggaat agcagtaatc atacttttaa
     2161 ttctaggagt tcttgcttat gttgattcga aaaacactga attacaagct aatgaaaatt
     2221 ttatcgaaac atatactatt cctgaaaatg aaaaaatatt tataaatggg atggtcgttc
     2281 ctaagcaaac taaggacttc aatatatctg gagattatga gttaagtgat gttaatgtta
     2341 caaatggtca aaaagtaaat caaggagacc ttctttttac tgctaaaaat ccaactataa
     2401 tagcagaaat agatagttta aaatcacaac ttagtcaata caaaaaacaa aaaatatcac
     2461 tttctgatat tgctgaaaat agagatgcta ttgcttctat aaatgcacag ataactgctt
     2521 taaactctca gatagcttct ctagataaaa aagcgtatga cagagttact gctccttttg
     2581 atggcacagt atacttgaat gaccaaactg gaaacccaga ccaaccagtt tcatttatga
     2641 ctattcaagg tctagagttt tatatgaaag gacaagcaag tgaacaagat ttacctaaaa
     2701 tgaagattga ccaaatggta aatatactag ttttttcaac tgaccaaaag cttactggta
     2761 gaataagttt tatatctgat aaaccatcta ctccaaatac tgaaatggga gctcagcaga
     2821 atacactatc ttattatgat ataaacatag catttgataa tcaagaagga ttagtaaatg
     2881 gattccatct tcaagcatca cttgaagtag ctaattcaag ttttaaaata ccagcatctt
     2941 gtgtacttaa agacaacaaa cactcatatg tatttaagga tttagatggt atattgaaaa
     3001 agcaaattgt agatgtagca agtcaaaatg atgattttgc tgtagttaga ggcggtcttg
     3061 aacaaggcga tataataata aaacacccta ctaaagaaat gaaagaagga gacccagttc
     3121 aaggcgatgg cgtaactgct ggttctaata atggagatac tactccaaac aaaaaagtag
     3181 aagaagttac tatggatgtg aattaattta catatgttaa ttaaattaga aaatattcaa
     3241 aaatattaca aagtagggaa agatgaatta catgttttaa aatccttaaa tttagaaatt
     3301 gaatctggag aatttgtcat gataatgggt aaatctggta gtggtaaaac tactttactt
     3361 aatatcttag gttttctaga tgtatttgat gaaggaagat atatatttga tggaacagat
     3421 gtaactaatt tgagtgaaaa tgaacgttct gtttttagaa atattaatat tggatttgtt
     3481 ttccaacaat ttaatttaat agaaactcta aatgtatatc aaaatgtaga gttgcctcta
     3541 atttacaata aagtactaaa aaaatctaat agagaagaaa ttgttaaaga taaattaagt
     3601 tctgttgggc ttttggacaa acttaagcag aaaccacttc aattatctgg tggtcaacaa
     3661 cagcgtgtag ccattgcacg ttgtctagca aatgaccctc agataatctt tgcagatgaa
     3721 ccgactgggg cattagatag tgaaactagc agagaaatta tggagctttt aacaaggctc
     3781 aacaaacaag gtaagactat aataatggtt acacatgatc aagatttaac taagtatgca
     3841 actaaggtta tacgacttaa agatggtgtg tttacttcgg aggtgtaatc atgagtttat
     3901 taaaaaattc tttggccaat ttaaaaggtc acaagcttcg tgtatttgta gctttactct
     3961 ggattataat aggaataact tcagtaatcc ttgttagttc tataggtaat ggatttcaaa
     4021 aagaaattaa gaaatctgta aacaatgtaa atcctaataa aactactatt tcgtttgaat
     4081 cagccgataa cacaggtctt actgatgata tgagtatttt tttaaaacca tttaatgcaa
     4141 aagatttaga agaattatca tttgttgaag gagtagaaag aatagctcct tcaagagatg
     4201 ggtttaacct tgattcagtt tactcttcac aagcatcttt tgataaaaaa actacgtatg
     4261 ttgatgttgg accagtaaaa aaagattcta aaataaattt aatttgtggt agagattttt
     4321 ctttagatga tgaaaaaaga aaagtaattt tacttacatt acaaagtaca agtgaaattt
     4381 ttgaaaatcc agaagatgcc ttaggtcatg gtatcaacat aaatggaact atatttgaga
     4441 taataggagt attagatgat tctcagcaaa accaagctgg aggattcttt ggaggttacc
     4501 aagatatgca atttactact tccctagtac ctaaaaaggc ttttgacact ttaatgagtc
     4561 aaaattctta ttcaaatgaa atttaccagt tagacttagt ttcttctaaa ggctataatg
     4621 taaatgaagt tgcaaacaat gttatagcta aattatatga gatgcaccct ggtatcaatg
     4681 gttcttatac tacaccagac ccaactgaac agacagctta tcttgaaagc attaattcca
     4741 atgtaaacaa atatgtatct ataataactg tagttgcaat gtttgttggt ggtattggtg
     4801 taatgaatat aatgtatgta tctgttatgg aaagacaaag agaaattggt ataagacgtg
     4861 ctattggagc aaaacctagg tctatattat ttcaattttt agttgaagct gtttttataa
     4921 cagtatgtgg tggaatttta ggaactatag ttggatttgc tgctactaac tatgtttcta
     4981 aatatatagg ttttgaagcg atacctagct taaatagctt attttatgca attgtagcta
     5041 ctatattgac tggagttgta tttggtctaa ttccagcctt taaagcctct aaactagacc
     5101 ctataaaggc aatttataag tagaaattgt ataaagattt tcgattttta gattaattta
     5161 tataaagcat acttaaatat ataacgcact attatacatg atagattaag aacttaatct
     5221 ttccaaatat aatagtgcgt tctttcataa aaaggattct cttccttact atatctaaat
     5281 taccaatata tcatgttcta attgttcaaa ctaatacttt caatttgaat taatcaatct
     5341 cattctccta atatacaatc ttttactttc aagcattgta ctctctgcct ttaaaaaata
     5401 atcttaaatt aggcgttata tacaatctcc ccattacata ttgtataatt aacttttcca
     5461 tataaagatt tacctttaaa tggtgaatta tcagctttag aaacaaagct ttcaactgtc
     5521 cattcttctt ctggattgaa tataacaatg tctgctacag ccccttcttc tataaatcca
     5581 atatttaaat tgtacaactt tgcaggattt gtagacattt tttctattaa ctgcatcata
     5641 ctaagatgat ttttatgcac caaatttgtt attccaagag ctaaagaagt ttcaagccca
     5701 ataattccac ttggagcttt tataaactct ctatcttttt cttctttact atgaggtgca
     5761 tggtccgttg ctattatttc aatagtacca tcatttaatc cctctataat cttatctcta
     5821 tcccatttac ttcttagagg tggattcatt tttgcatttg ttccaaactc caatacatct
     5881 tcctcagtca atgtaaagtg atgtggtgaa gcttctgcaa ctacatttgc acctaagtat
     5941 tttgcaaatc taaccatatc aacagataca cctgaactta tatgttgtat atcaacctta
     6001 gcaccagtct ttaatgccaa catacaatct ctggctacca taacatcttc tgccacactt
     6061 gatgcacctt ttaatcctat ctctgaagct acctttcctg catttacacc tggatttcca
     6121 actaaagaag ggtcttcttc atgaaaacta agtggtacat ccaactcttt tgccttaatc
     6181 attgcatcca taattagatt gctatccatt aaaggtaatc catcatcagt aaatcctggt
     6241 acaccagctt ttttaagagc ttccatatca actaactctt taccttcaaa ccctttagtt
     6301 atagctgctg cttgtaaaac atttatacat gcattttttg acttttcttt aacataatta
     6361 aaagtatctt cattatctac aataggatta gtatttgcca tacatataac agttgtaaat
     6421 ccacctctag cagcagattt agctccactt tctatatctt ctttatatgt aaacccaggg
     6481 tctctaaagt gtacatgtac atccactaaa cctggcgcaa ctacattacc actagcatct
     6541 attatttttt catattcatc actttcatca aactttccta ttttataaat tttattttct
     6601 tttataatta agtcaatatt ctcatctctt tgacttttag ggtctattac tctaccattt
     6661 ttaattaaaa tcatattatt tctcctttat atttttaatt tatatagact catataatat
     6721 tatacattat tttacatact taaatacaaa attacgtttg acatcaatag cctttttagg
     6781 acaaagttca tggcaacagt aacatcttat acatttactt aagtctataa tagcaccctt
     6841 agtccctatc tctatagtgg atgcaggaca agcttccttg cattttccac attttataca
     6901 atcttgaaat ctaaccacag gctttggtgt tataagcttt gaaataggtt catttaaaaa
     6961 ttttggtagc tttacggaaa acaatcttaa atctttactt acatttggaa ccttgtaatt
     7021 ttttacaatt aaatctttta tatcttcacc taaaatttca atatctgaaa aatcttcttg
     7081 tatatagcct ctcttaatag cacctctaat tgtagcaact ctatttggaa gtaaacttat
     7141 aatcttacat gcaacaacat ctatagcata tggattttgt gatgctaata aaactcctat
     7201 ttctcgagta gtaccagctg aaggtccatc accttccata cctactatac catccataat
     7261 tgtcaatgtt ggttttatat aatcacaaat atctaaaagt acattttcac aaaacagctc
     7321 ttctgtagga aatctataat gtatctctgc ttttttcaat ccagctatac atccatataa
     7381 gttttttgta ccaccagtaa aagttgccat tatatgagtc tttaatttac aaagatttat
     7441 tatgtaatct gaatctacta ttggtttaat aatatccata tattttagaa catgtgcttt
     7501 ttcactatgt atcttaacat ctgaaacatc ataatttaac ttaatgctca gttcatttgc
     7561 tacttcttca attccagatg ccttataaat gctcttaaga gaactttgat tatatggacc
     7621 accagggcta tctgctattg taatatcaca atttaactct aaaagttttt cacatacaac
     7681 ttttacaacc ataggatgtg tagttgtagc ttcttcaggc ttttttttca tcaataaatt
     7741 tggttttata agtactttac ttcctttttt aacaaactta tctataccac caatatcctc
     7801 taaatttttt tcaatagata actttacttc ttcataattg taagttttac aactcctaac
     7861 agataccgac ttcatattat ctccatcctt ctatcacaac agtttatttg tataatttaa
     7921 aaaaggagaa accaaagttt ctccttttaa tctatgtttt aattattatt ttccaaaatg
     7981 tctgtttaat aatcctaaga atgctttacc atgtctagct tcgtctttac acatttcatg
     8041 tactgtatca tgtatagcat ctaatcctaa ttctttagct ctcttagcta attttaattt
     8101 tccgtcagtt gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc
     8161 aactacaact tctccaagaa gttctgcaaa tttagcagca tgttcagctt cttcaaaagc
     8221 tattctctta taagcttcag ctacttctgg ataaccttct ctatcagctt gtctactcat
     8281 tgctaaatac attcctactt ctgtacactc accagtaaag ttggctctta atccctcgat
     8341 tatttctgca tctacacctt gagctactcc tattctatgt tcatcagccc aaaccatttc
     8401 gcctttcatt tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc
     8461 agcatctcct tcatgtatat atccacatac tgtacaaaca aattttttca taattaattt
     8521 cctcccaatt ttatataatt aaatttttat ttttaagttt aataacttgc tacaagaata
     8581 tatatacccc agactttata gcttaaacac taaaaataaa atttatttcc taattaattt
     8641 tatcactttg aaacaccagt atcaaccaag ttttgatgtc aaatttctac ataatacata
     8701 atttatttga ttaattagga aataattgtc atattaaata ctttctaatg aagactcttc
     8761 tgaatcctta agtttcactt ttttgctctt taaaaaagat aatttagttt ctgcagttat
     8821 gattgcaaca aatatcaaga tgctaccaat aaccaatttt gctgtaataa gctcctttag
     8881 aattattata gaaaatattg taccaaatac agcttctgtg gataatatta tagctgttct
     8941 agttccatca actctttttt gacatatagt ctgaaataaa aatccaattg tagtactaaa
     9001 tacagctagg tacatagttc ccatgtaacc agataattca gcttccatct ttaattgacc
     9061 tgcaaatgtt tgaactacaa cagacattaa aaacgctact gtaaattgaa tagctgtcaa
     9121 tgccattggg ttgttatctt ttgcaaactc acttgtaaag aatatatgaa atgcaaatcc
     9181 gaatgaacat ataagtgtta gaaaatctcc aaaattaata gagaaatcag cttctaaaga
     9241 aagtatccca attcctataa gagctaccaa actacttatt attcctatct tatcaagttt
     9301 tcttctataa agtataaatc ctatgaatgg aactattaca acattagctg ctgttataaa
     9361 cgcattttta gatggtgtag tgtacataag tccaatagtt tgtactgtga aggctagaaa
     9421 taaaaatatt cctaaaaccc ctcctgcttt taatagtttt ttactcatat tggcacgaat
     9481 ctgtttaaaa aacagtaaat tcataattat agctgccaag aaaaatctta aagttatgat
     9541 ttgtagaggt gtaagcccac cgtccaatgc aaattgggtt gctacaaagc cacttcccca
     9601 tataattgct ataaatatta gtcctatttc tccaaagtat tttttcaata aagccccctc
     9661 ctttttattt ttttgcaaaa ttataaaccc cctaaacagc aagagagttt ttctataagc
     9721 attactataa attaatatag taaatttttt cagtatttta aatctaaatt tacatacatt
     9781 ttgagctcaa atttatatcc actatttaac attaatatat taatcatttt ttgtaaacac
     9841 ctttttaata aatttttata ttttttatat ttttttacct atactaaatt tttttata
//
LOCUS       DAEMAE010000134         9879 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEMAE010000134 DAEMAE010000000
VERSION     DAEMAE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.136"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            81..1556
                     /locus_tag="KPH93_002554"
     CDS             81..1556
                     /locus_tag="KPH93_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG1689361.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVAAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLERIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1643..2056
                     /gene="speD"
                     /locus_tag="KPH93_002555"
     CDS             1643..2056
                     /gene="speD"
                     /locus_tag="KPH93_002555"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG1689362.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2086..2937
                     /gene="speE"
                     /locus_tag="KPH93_002556"
     CDS             2086..2937
                     /gene="speE"
                     /locus_tag="KPH93_002556"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG1689363.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCADDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2927..3805
                     /gene="speB"
                     /locus_tag="KPH93_002557"
     CDS             2927..3805
                     /gene="speB"
                     /locus_tag="KPH93_002557"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG1689364.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     IPFMIGGEHLVTLPAFKAVYEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(3985..4185)
                     /locus_tag="KPH93_002558"
     CDS             complement(3985..4185)
                     /locus_tag="KPH93_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1689365.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4652..6142
                     /locus_tag="KPH93_002559"
     CDS             4652..6142
                     /locus_tag="KPH93_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1689366.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDIMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANAKIKDFSNINLEK"
     gene            6422..7918
                     /locus_tag="KPH93_002560"
     CDS             6422..7918
                     /locus_tag="KPH93_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1689367.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEISLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKSKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8052..8966
                     /locus_tag="KPH93_002561"
     CDS             8052..8966
                     /locus_tag="KPH93_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1689368.1"
                     /translation="MKKIILKDILFLVTTLSIIVILLLVTNITANSKIKSTSETYLKS
                     DKNSVSQNGIQSNTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVARL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIQPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKIPHHGRYDKLTEAFLESISPQCAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            9084..9275
                     /locus_tag="KPH93_002562"
     CDS             9084..9275
                     /locus_tag="KPH93_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689369.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            9498..9848
                     /locus_tag="KPH93_002563"
     CDS             9498..9848
                     /locus_tag="KPH93_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689370.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         3950 a         1041 c         1783 g         3105 t
ORIGIN      
        1 aaagtaaaaa taaaaaatag aaataataaa taataaatat taaaaatatt tgaatataga
       61 gaggagatga atttgaaatc atggatagcg agtttgcaaa tgcagttgaa ttaagctatg
      121 agtgtgctcc attattagag tcgttaaaag aatactcaga gaaagacata gcttgttttg
      181 atgtgcctgg acatgtaaaa aatagaggtg tagctatatt aaataaatat ctaggtgaaa
      241 gtctcatgaa aatggatata aattcatcac caactatgga taatgtagct gccccaaatg
      301 ggattataaa aaatgcacaa gatttgctag cacaagcata tatggctgat gaggcattct
      361 ttataacgaa tggcacaact caagcaatac atgcaatgat tttgagtgtt ataaaaccag
      421 gtgaaaaagt attacttcca agaaatatac ataaatcagt aataaatgcg ttaatacttt
      481 gtggaggaat accaatattt atacaaccag aatttgatga aaaactaggt ataagtttaa
      541 atattactct tgaaaaagtt agaacagaga tagaaaaaga ttgtaatata aaagcattat
      601 ttttccttaa tccaacttac tatggagttt gtgcagacct tgagcgtatt atagaactat
      661 gccataaaaa taatgtttta gtgttagttg atgaagctca tggtgcacat tttccatttc
      721 atttagattt acctccttct gcaataagtt tgggagctga catggtagct gtaagtatac
      781 ataaaactgg aggagcattg acacaatctt cagcactctt attgaataga gataatgtaa
      841 gttttgaaaa ggttcttcaa tcaatcaata tgctacaatc gacatcagca tcttatttac
      901 ttatggcaag tatagatggt gctagagtta atctagttga gaatggtgaa aagcaactgt
      961 caaaagcatt aaatctatct aggtatgcaa aatctaaatt gaacaaaatt gatggaataa
     1021 aagttttatc tacagaaata ttgaaacaaa aaggtgttaa atttatagat gaaacaaaat
     1081 tgtgtataaa tgtaaaagaa ttaaatttga ctggatttga agtatacgat ttattatata
     1141 aaaatttttc aattcaagta gaattgggag attcatataa tatactggca cttgtgtcca
     1201 ttggaacaaa taaatcagat atagatagat tagtaaaagc actttctata attgcaaaga
     1261 tttatagaaa agaatcaaca ttaaatgaat ttaatatggt tcaaataaat ccaattatta
     1321 aacttaatcc aagagaagct ttttatgcac caaaggaaag tgtagaaatc aattcatgta
     1381 tagatagaat ttgtggagaa tcaataatgg catatccacc aggtataccg attattgctc
     1441 caggagaatt gataacagaa gaaattatgg agtatattat tttcttaaaa aatagcaatg
     1501 cgtatcttac agatgtacaa gataaaaact tagatagaat actggtaata aaataaatta
     1561 ctttattttg caagggcaag tgtttgttaa gtagagttta tcaaatagaa cttattaaaa
     1621 gtataaagga gttggggttg aaatgaaatt tgaaacacta ggaagacata tactagtaga
     1681 atactataat tgtgatgaaa acgtacttaa agacccacgt cttatagaaa attttatgaa
     1741 tgattcagca ttaaatgcaa aagcgacaat tgtggattct gtattccacc attttaatcc
     1801 ttggggggtt tcaggagctg ttataattgc tgaatctcat ttgacaatac atacatggcc
     1861 tgaatatggt tatgcagcag cagatttttt tacttgtggt gatatagacc catggaaaag
     1921 ttttgaatta ttagagcaac ttttaaagtc tgaaagaagc gagtctacag aaattccaag
     1981 aggcttaact acaaaaatta aaaaatattc aaaaaaagat ttaggtaaga ttactcataa
     2041 accagaagca gtataaaaca tacaagagaa agtgaggatt attatatgga actttggtat
     2101 acagaagaat ggacagaaaa tgtaagattt tctataaaag taaataaaca tctatttgaa
     2161 ggaaagtctc aatttcaaag gatagatgtg tttgatagtg atgaatttgg aaaatttcta
     2221 acaatagatg gattaatgat ggtaacatat aaagatgaat ttatatacca tgagatgata
     2281 actcatgttc ctatggctac aaatttaaac ataaagaaag tattagtcat aggtgggggt
     2341 gatggaggta cagtaagaga attaagccgt tatcctcaaa tagaaaagat tgacatggtt
     2401 gaaatagata aaatggttgt agatgtatct aaggagtata tggatatatg ctcatgtaaa
     2461 ttagatgata aaagagtaag tttatatttt gaagatggtg ttaattttgt taagtgtgca
     2521 gatgataaat catatgactt gataatagta gattcaactg acccaatagg accaggcgaa
     2581 gggcttttct caactgattt ttataaggat tgttatagaa tactgactga tgacggaatt
     2641 ttagtaaatc aaagtgaaag tccatatttt gactttaatg ctaaagagat gaaaagagct
     2701 aataaaaaat taaaacagat atttccaata tcagaagtct atcaagcaca tataccaaca
     2761 tatccatctg gtcactggtt gtttggattt gcttcaaaaa aacttaatcc agtaaaaaat
     2821 caagatagaa atggatggga gaaattaagt ttaaaaacta aatattataa tagtgatata
     2881 catttaggtt cgtttatgtt acctcaatat gtgaaggaga tgctagatga agaataattt
     2941 ctatcatatg aacaccttta tgagtatgga caaaaattat gaagaatcta atcttatagt
     3001 atttggtgtt ggatttgatg gaactacttc taacagacct ggggctagat ttgcaagtag
     3061 ctctatgaga aaagaatttt acggtcttga gacatatagt ccttttttag atttggattt
     3121 agaggattat aatatatgtg attatggaga tttagaaatt agtgttggaa gtacagaaca
     3181 agtcttaaaa gaaatctatc aagagacata taagattgtt agagattcaa agataccctt
     3241 tatgattgga ggagagcatt tagttacatt accagccttt aaagcagtat atgaaaagta
     3301 caatgatata tatgtaattc attttgatgc ccatactgat ttgagggaag aatataataa
     3361 tagtaaaaat tctcatgcaa cagtaattaa aagaatatgg gatattgtag gcgataataa
     3421 aatatttcaa tttggtataa ggtctgggac aaaagaagaa tttaaatttg ctacagaaga
     3481 aaaacacaca tacatggaaa taggaggaat agatacattt gaaaatatag ttaatatgct
     3541 aaatggaaag aatatttatc taactataga tttagatgta ttggatgcat ctgtatttcc
     3601 aggaacaggt acaccagaac ctggtggtgt aaattataga gagtttcaag agatttttaa
     3661 gattataaaa aactctaata taaatatagt tggttgtgat attgtagagt taagccctga
     3721 ttacgataca acaggtgtat cgacagttat agcttgtaaa atcttaagag agttatgctt
     3781 aataatatct gataaaatta aatagagtat tttatataaa taatatatat tataatataa
     3841 gaagttattt taaaaaagtt agtttaagtt tgacgctttt ctaaagtaac ttcttatttt
     3901 tgtaattaaa aataaatata ataaacaaca aaaaagagcc ctcgatagct ccttttctaa
     3961 aatatatata tttaaattaa ataattatat tctatttacg ttttcagctt gaggtcctct
     4021 attaccttga gttatatcaa agctaacctt ttcgccttct tctaaagttt tgtatccatc
     4081 attttgtata gctgagaaat gtacgaaaac atcatcttct ccctctacag atatgaaccc
     4141 aaatcctttt tcattgttaa accattttac tattccgttt ttcattataa gaaaacctcc
     4201 gcattattaa aaaatacaaa ctaaaaaaat aagtctgttc tattaatata acataaaatt
     4261 taaataaaag caaataaaat ttttaaaaaa attaattact tttagaattt aataaaaatt
     4321 gttaaattat atagtgagaa gtattgtaaa atttgacgaa aatgcaaatg taaataataa
     4381 atttaatatc aaaaaatgta acaaaaaggt gtgcaagtaa aaagtaaaat taaaatatat
     4441 aataaaaaat attatatatt agattgaaaa gtaaaaataa aaatagtgaa atttcagaaa
     4501 tatcaataat attatataat aaaataaaaa aacactatat atattcgata aaataaaatt
     4561 taaacatata atattttatg tatatattaa taaaaggaca tattgtgtta taatggtatc
     4621 gtataaaatc atttacgggg gtggtattaa aatgtttcaa tttgaaacac aaatacacaa
     4681 attaagacat gatgttttaa ctgcaatagc tagacttgga aaagaagata accttaccag
     4741 agaagcaatg gataatattc aatatgaaat aataaaaggt gagaaaccta catacagatg
     4801 ctgtgtttat aaagaaagag ctataatatc tgagagagta aatgtaagta tgggattaca
     4861 accaggtcaa gataagatag atttagatat aatggatata gaagaagatg aacaaattat
     4921 atatattttg gaagctgcat gtgacagttg tccaataaat gaatttacag taacagatat
     4981 ttgtagagga tgtctagcac atagatgtaa agaagcttgt aaatttggag ctataacaca
     5041 tgttggagga atggcctata taaatcatga attatgtaag gcttgtggaa tgtgtaaaaa
     5101 agcttgtcaa tatgatgcca tctcagaggt tgttagacca tgtaagagtg tttgtccaac
     5161 taatgcctta ggatttgata gagaaaatat gaaagctatg atacatgagg ataaatgttt
     5221 aaactgtgga gcatgtatgt cagcatgtcc atttggagca atatctgata agagtttgat
     5281 agctcctgta gctagaaaat tagttcaaaa agagaaaatg tatgcagtag tagctccagc
     5341 tataacaggt caagttgaag caaatgttac atatggtcaa ataaaaaatg ctataaaatc
     5401 actaggattt gttgatgtat tagaagcagc ttgtggagca gatgcagtta cagttcatga
     5461 aagtttagaa tttgctgaaa gaatggaaaa gggagaagct tttatgacaa attcttgttg
     5521 tccagggttt gttggttata tagaaaaaac atacccaaat catgtaggaa aaatatcttc
     5581 tacagtgtct cctatgatag ctagtggtag atttataaaa agtcatgaac cagatgctaa
     5641 agttgtattc ataggaccat gtacagctaa aaagagtgag gcagctaaga aagaattaaa
     5701 aggagctgtt gattatgtac ttacttttga agaaataatg gcattatttg aggcatttga
     5761 tatagacttg gctaagtgtt caaatgaaga tatagatgat gcatcaattt ttggaagagg
     5821 ttttggagct gccggtggtc ttacaaaagc tatagaaaat tatcttgctg aaaaaggtat
     5881 tggtgttaat tttgaacctg taaagatttc tggttcaaga gaaatcaaga aaactatgac
     5941 tatggctaat ttaggaaagc tagaaggtaa ttttatagaa ggaatgatgt gtgaaggtgg
     6001 ttgtataaat ggttctggta aattcttatc aggtgcaaag gttaaagcca cttttgatag
     6061 aaaaaattct caatcaacta agaagagtgt cttagcaaat gcaaagataa aagattttag
     6121 taatataaat ttagaaaaat aaagataata caaagcaaca aaaatacata ttgttaatag
     6181 agttgttcta agttgaagtt taacttttgg acaactctat ttttatttat atatttaatt
     6241 attgaacaac taaaataaaa attatataca cttaaaataa aaattacata tataatattt
     6301 tgtatataac ttaacaaagt atatgcctat gttataataa tttatagaaa taaataaaaa
     6361 atacaatata cataatagta gaaatatagc aaaaaggtgt gatttatagg gaggattagt
     6421 tatgtttgaa tttgaaacgc aactaaaaag gttaaaacat gaagttttaa cagcagtagc
     6481 aagactatct attgaagaaa atttaacaaa agaaaatata gaaaaaattc catatgaaat
     6541 aattaaagga gaaaaggcga gatatagatg ctgtgtttat aaagagagag aagtagttct
     6601 tgaaagggcg caacttgcat taagtttaac tccaaatagt aaatatggag atataaatcc
     6661 agattcagtt agtttggaca cagatgagca aattatatat atagtggaag cagcatgtga
     6721 tagatgtcca ataaatgaat ttacagttac agagctttgt agagggtgtt tggctcatag
     6781 atgtaaggaa gcatgtaaat ttggggcaat ttcttatata aatgggagag catatataaa
     6841 tcatgattta tgtaaggctt gtggaatgtg caaaagttca tgtcaatatg atgctatatc
     6901 agaagttgta agaccttgta aaagtgtttg tccaacagga gccttggatt ttaatagaaa
     6961 cactatgaaa gcaatgatac atgaagataa ttgtataaat tgtggagcat gtatgtcagc
     7021 atgtcctttt ggagcaatat ctgataagag tttgatagca cctgttgcaa aaaaacttgc
     7081 aaagaaagaa aatatgtatg caatagtagc tccagctata acaggtcaaa tagatagtaa
     7141 tattacatat ggtcagttaa aaaatgctat aaaatcactg ggatttgtag atatgataga
     7201 agcttcttgt ggagcagatg cagttacagt tcatgaaagc aatgagctta ttgaaagaat
     7261 aaaatcagga gataattaca tgacaaattc atgttgtccg ggatttttta agtatataga
     7321 gaagatgttc ccagatgaaa aagatagaat atcatctaca gtatctccta tggtagcaac
     7381 aggaagatta gtaaaaaaaa tagattctga tgccaaagtt gtatttatag ggccatgtac
     7441 agctaaaaag agtgaggcaa cagagataag tttaaaagat tcagtagatt atgtacttac
     7501 ttttgaagaa ctaatggcat tatttgaagc atttaatata gatttatcaa aatgttcaaa
     7561 tgaagatata aatgatggtt cgatttttgg aaggggattt ggagcatctg gtggacttgc
     7621 aaaatcagta cagaactata ttgctgaaaa ggggcttagt tttgattttg aacctataaa
     7681 agtatctggt ccaagacaaa ttaaaaagac aatgacaatg gctaaattgg gaagattaga
     7741 aggtaatttt atagaaggaa tgatgtgtga agaaggttgt attaatggag caggtaagtt
     7801 tttatcaggt gtaaaatcta aaaatacttt tgatagaaaa aatggacaat ctactaaaaa
     7861 gagtgtttta tctaatgata agattaaaga atttgaagat gtaaatttag aaagataatt
     7921 gttttggtat agtcattttt ttatcatgcc acaaatatgc ctagaatttg ccctttttat
     7981 atgtgtattt tacagagtta agattgctat actataaata tgtaataaat aaaatttata
     8041 ggagcttttg gatgaagaaa ataatattaa aagatatcct gtttttagtt actacattat
     8101 ctataatagt aatattattg cttgtaacaa atataactgc taatagtaag ataaaatcaa
     8161 caagtgaaac ctatttaaaa agtgacaaaa atagtgtgag tcaaaatggc atccagagta
     8221 acacaaaaaa tactgcattt aagactacta tactaagcac tggaaagtca gattgtatat
     8281 tagttgagat aggtaacaaa gtcataatga ttgatacagg agaagataaa aatggaaaac
     8341 aaatagttgc tagattaaaa gaaaaaggaa taaatacact tgactatttg attttaaccc
     8401 atctagataa ggaccatata ggtggagtag atagtgtgtt atctagtgtt aaggtaaaga
     8461 atattataca agcaaattat aagaaagata gtaagcaata tgatgaatat attgactcac
     8521 ttaaaaaagc tgatatagaa ccagtgctac taaaagataa tatgaatatt gtaatcaata
     8581 gtgcagaaat aaacatacag ccggcttcaa aaagcaagta cgagagttca aatgattatt
     8641 caataataac taatataagc tatggagcac ataagttttt atttgcagga gatgcagagg
     8701 aagaaaggtt gtcagaattt ataaatggaa ataccttaaa atatgatttt gttaaaatac
     8761 ctcatcatgg aagatatgac aaattaactg aagcattttt agagtctatt tcaccccaat
     8821 gtgctgttat tacatgttca gagaaaaaag aaccagagga aggcgtttta aaaatattgg
     8881 aaagattgaa tataaaaaca tttttgactt ctaatggaga ggtagttatc aacagtgatg
     8941 gtaagacatt gagtgtgaat caataatttt tttaaaaata tctaaaaaaa tttaaaaaaa
     9001 tcattagcaa atattacccc ttaaacatag tatattttag gagctgaaac tccacataca
     9061 aaatgactag gaggtaacat attatgttgg atagattttt tggtgaaggc ggatttggtg
     9121 gatgtgcttg gtggataata atattattct tcttattctt agcattccaa gattgttggg
     9181 ctgaaatgga cttatgtgca tggatacctt tcttaatatt attattaata ctttgtgaat
     9241 gtggtgacgt ttttgatgga ggatgtggat gctaaaaaaa tcaatttaga tgatttaaca
     9301 aagcttatgg tgggtataaa caaaatacca aaccaatcac aatctagtgt ttgaaggtag
     9361 tcggaaatcc cccgctacct tctttttttt attgttaaaa tttatttttg aattatttca
     9421 tttacatata ataattttta cgataaagtc tgatataatt aaaaagaaaa taaaatttat
     9481 ttataggaga tgtaaaaatg gctatagtgg ataagatttt caatgagata aaacttatgg
     9541 ttgcaagact tttattggga aaaaaataca gtgaatatga agaaaaagat ttagtgtata
     9601 atacagaaga agaagttata cttataactt taaaaagatt agtttttcag ggaaacgtaa
     9661 atgaagcaga agaaatttta tttgataaag ctaagagtgt aaatagtgaa aatatgcagt
     9721 atatagcaat agaattttat actatgctta tggaaaaaac tgatgaagaa ttagaagcta
     9781 tgaatttttc caaacaagaa gtttatcaag gtatagaaga tattagaaaa gtattgaatt
     9841 tagaataaaa gatatatgaa aatattgaaa taaattaat
//
LOCUS       DAEMAE010000135         9856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEMAE010000135 DAEMAE010000000
VERSION     DAEMAE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.137"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            349..915
                     /locus_tag="KPH93_002564"
     CDS             349..915
                     /locus_tag="KPH93_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1689371.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNARGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            955..1536
                     /locus_tag="KPH93_002565"
     CDS             955..1536
                     /locus_tag="KPH93_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1689372.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1593..2510)
                     /locus_tag="KPH93_002566"
     CDS             complement(1593..2510)
                     /locus_tag="KPH93_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1689373.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPFIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRNVNSL"
     gene            2640..4271
                     /locus_tag="KPH93_002567"
     CDS             2640..4271
                     /locus_tag="KPH93_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1689374.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTISTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTVIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEMAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4274..5221
                     /locus_tag="KPH93_002568"
     CDS             4274..5221
                     /locus_tag="KPH93_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG1689375.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5506..5769)
                     /locus_tag="KPH93_002569"
     CDS             complement(5506..5769)
                     /locus_tag="KPH93_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG1689376.1"
                     /translation="MEYSYSKINLKKGDIVEVNLEKQANVILLDHINYVKFKNQKNYD
                     YYGGFAKKNPCRMRVPNTGIWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5868..6113)
                     /locus_tag="KPH93_002570"
     CDS             complement(5868..6113)
                     /locus_tag="KPH93_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689377.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            complement(6538..6906)
                     /locus_tag="KPH93_002571"
     CDS             complement(6538..6906)
                     /locus_tag="KPH93_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG1689378.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            7351..8307
                     /locus_tag="KPH93_002572"
     CDS             7351..8307
                     /locus_tag="KPH93_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG1689379.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQGIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTEDMLEKLSEKGIGK"
     gene            8525..8872
                     /locus_tag="KPH93_002573"
     CDS             8525..8872
                     /locus_tag="KPH93_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689380.1"
                     /translation="MLNLKEIRKEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKNIENVLLDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            8912..>9856
                     /locus_tag="KPH93_002574"
     CDS             8912..>9856
                     /locus_tag="KPH93_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG1689381.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTL
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNDSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKN"
BASE COUNT         3640 a         1296 c         1458 g         3462 t
ORIGIN      
        1 gttgttttta agataaaaat taaatgtatc aattattgtt ttatacaaat atttccaaac
       61 ttgctctcat ttaaatagta tgcttagaaa ttttagaatt attgactcta actttattac
      121 ttacttcact ttataacaat caaatacgag tttaaatatt ataaaactac aaaataattt
      181 aaagcatata atgtcaatat gtttaaatta taaaatattc atgtagttat ataataaata
      241 atttataaat atagccaact tagcctaaat acgtatataa tatagctaat atggttctta
      301 tacttgatac catcaataaa actaaaaata taaaaaggag aacttaaaat gagtgttagt
      361 ttacaaaaag gacaaagagt tagcttagta aaagataata atcctgtaaa aaatctagta
      421 gtaggcttag gatgggatat gaataaattg ggtaaaaaaa actatgacct agatgcattt
      481 gcaatagcat tgactaacca agataaaatg aatgctagag gtgacttggt ttattttgga
      541 aatcttaaac acccttctaa agctattata cacactggag ataacttaac tggaaaaggc
      601 gaaggtgatg atgaacaaat aattgttaat ttagaagaca tacctgaata tgtacataaa
      661 atagtttttg gagtttctat atacaaagct aaaaaaagag accaggattt tggtcaaatg
      721 aacaactctt ttataagatt aattgattca aattccaagc aagaattatt taaatataat
      781 cttcaagaag acttttctgg taaagtaaca gttttggcag gagaaattta tcgtagaaat
      841 gaagaatgga aatttaatgc tttaggaatt ggtcaaaatg aagagcttcg tgaattaatt
      901 aatacatata agtaataatt acaataaaaa atttataatg aggagttggt ttaaatgggt
      961 ataactttag caaaaggaca aaaagtaagt ttaacaaaaa gtaaccctgg tctaaaaaaa
     1021 gtaatagtag gactaggatg ggatattaat aaatatgatg gtggctttga tttcgattta
     1081 gatgcttctg catttttaac tggaactgat ggaaaagtaa cgaatgatgg agactttata
     1141 ttctataata atttaaaaca cgcttctgga gctgttgaat atatgggtga taatagaaca
     1201 ggtgttggtg atggtgatga tgaacaaata aatgtagact tatctaaaat acctcaaaat
     1261 attgctaaaa tatcatttag tgttactata aatgaagcaa ttacaagaag acaaaatttc
     1321 ggacaagttg aaaattcata tataagaata tataatgaag aaactaatga ggaacttatt
     1381 aaatatgaat tgggtgaaga ttttagcata gaaacagcaa tagttgtggc agaactatat
     1441 agacataatg gagagtggaa atttaatgct ttaggttctg gatttgagga tgggcttgca
     1501 ggattgtgta aaaattttgg cgttaatata ggctaatata tctaaataaa aataagagta
     1561 tattgatata ctcttatttt tatttagata tattataaac tgtttacatt tctactcttc
     1621 tttctcgctt gttttttccc atattctact gaaatatcaa aaaatgctga aatgttttct
     1681 gtaaaaaact tgcttctatg atatgcaata tcaaatgtcc tatgtaagtt aataccttca
     1741 atatcacaag cccacaaaag atgctttttt aaataatcct caataagtaa ttcagatata
     1801 actgcaatgc caaaattatc tacaactgct cttaatatag cttcagagtt ataacatgac
     1861 catctaatat tcattgggat ttttaattct tttaattgct tttctaattg tgctcttgta
     1921 ccacttccta attcacgcat aattaatggt tgatttgaaa gttctgaaac ttttatagag
     1981 tctcttttgt agaactcatg cttgtgtgaa catataagta caagattatc attaataatg
     2041 ctttttgtaa caatatctgg gtgcttaatt tttccttcta caatcgcaat atctaattca
     2101 ctattaagta atttttttgc aatcagtctt gtatcttcta caaatacttc aggttctata
     2161 aaaggattta ccttgtatag ttctaacatt attgggctta ttacacatgt accaacagtt
     2221 actgttgccc caactcttat acggatattt tttgcacaat gcttcataca atcattcatt
     2281 tcttcattta cagctaaaag atgcctccca tatccaagaa gttttttccc tgcctcagtt
     2341 aaatataatt tttttgaaat tctatcaaat agcttaacac catactcttt ttctatttct
     2401 gaaatagctt ggctaactga tgactgggta atatacatat ttcttgctac ttcactcatt
     2461 ttaccacatt cagcaacctt aacaaagatt tcaagactac gaattgtcat attatcacaa
     2521 ccttagcaaa cattagtaaa tgcttatgat tttattaata ttttagtatt ttacaaatac
     2581 attgtcaaga agtataattt tattatagag ttataaagta gattggagtg tgatatattg
     2641 tgaaagtatt aattataggt ggagttgctg ctggaacaaa aactgctgca aaaataaaac
     2701 gtgaaatggg tgataattgc tctgttacaa tactaaataa aggcgatgat atttcttacg
     2761 caggttgtgg tcttccatat tacgttggta aaataattga agacaaatca tctttaatcg
     2821 taaatacacc tgaaagtttt tctaaactaa caggagcaga agttcgttgt ggagttgaag
     2881 ttacgtctat tgatagaaat gcaaaacaag taactttgaa aacgatttca acaggagaaa
     2941 aagaaaatct aagctatgac aaacttgtaa ttgctacagg ggcagaccca ataaaaccac
     3001 caatacctgg aatagattta cctggagtat tctttatgcg tactccaaat gatgctataa
     3061 aactaagaga ttctattgaa aacggtatta agagagctgt tgttattggt ggaggtttca
     3121 ttggtcttga aattgctgaa aatctttctg caatgggtat acgtgtaagt gttgttgata
     3181 tggctgaaca tgttatgcct ggatttgata ctgattttgc tgaatacgtt gaaaaccata
     3241 tggctgataa aggcataatg atttttacag gcgaccaggt tgtgggtatt gaaggtgaaa
     3301 ataaggttga aaaacttcgt actaaaaata gagctattaa aacagattta gtggttatgt
     3361 cagttggaat ccgtcctaac acaggttttc taaaagacac agggttagaa tttgctccta
     3421 ataatacagt tattgcagat gaatatatgc ttacaaatga caaagatatc tacgtagttg
     3481 gagactgtgc ttttgtaaaa aattctttaa caaataaacc tacatggtca cctatggggt
     3541 catctgcaaa tcatgagggt agaatttgtg cacaaaatat atctggaaaa tctaaaactt
     3601 acaatggtgt tcttggaaca acagtagtaa aattacctga gctcaatgca ggaaaaacag
     3661 gtctaagtaa agaaatggct gaaaaagaag gttataatgt ttgctctgta accatagcta
     3721 ctgatgataa agctcattat tatccaggtg catctaattt tattataaga atggttgctg
     3781 aaaaaaatac tagaaaatta ttaggtgttc aagttatggg acctggagcg gttgataaaa
     3841 tagtagatat cgctgctact gcaataactt taggagctga cctttattca ttagaaagta
     3901 tggaccttgc atatgcacca cctttttcaa cagctattca cccttttgta gtggcagtaa
     3961 atgttttaca aaataaactt aatggagaac ttgatagtgt tttacttgac gaaattgaag
     4021 attttgacag ttggactaaa cttgatgttt caaaagctcc ttctattcct agtttacgtt
     4081 atattcctgt tggtgaaatt aatggagaaa ttgaaggtct tgctaaggat gaaaaaattc
     4141 ttcttatatg tgctaaaggt aaaaatagct atatggcgca aaatcgctta agacgctttg
     4201 gctatacaaa tacgaaagtt cttgaaggtg gaatactatt ttatccaaat cttaaggtaa
     4261 tggaggagta aatatgagta atgttacagc agaacaaaaa aaggaagtaa aaggtagagg
     4321 ttttctacca agcagggatg gagaacattt tgcagcacgt ataataactg taaatggtgt
     4381 aataaatgca tcacaagcaa aaaaaattgc agaagctgct gaaaaattcg gaaacggtca
     4441 gttagcattt actactagac taacagtaga attacctggt gtaaaatttg aagatattga
     4501 agctctaagt gaatttatcg ctagcgaaaa tctaataaca ggtggaactg gatcaagggt
     4561 tcgccctgtt gtggcttgta aaggtactgt ttgtgttcat ggtctagctg atacacaggc
     4621 acttgccgct gaaatacacg aagaatttta caaaaattgg tatgatgtaa aactacctca
     4681 taagtttaaa attggcgttg gtggatgccc taataactgc attaaacctg acctaaacga
     4741 tcttggaatt gttggtcaac gtgtacctga ctatgattca gaactttgtg ttggttgtaa
     4801 aaaatgtgct gttgtagagg tctgtcctgt taaagcagct aaattaacag ataaaggtaa
     4861 acttgaaata gatagtaatc tatgtaataa ctgtggtaag tgtatagaaa gttgtaattt
     4921 tgatagtatt gaagaaaaag aatctggtta taaagtctat ataggaggaa aatggggaaa
     4981 atcagttcgt cctggaacac agattgatag attattttca aaagaggaac ttatgacttt
     5041 aattgaaaaa gcaattcttt tatttagaga acagggtaac acaggtgaac gttttggaat
     5101 aactatcgat agaatcggtg ctgataaatt tatagaaatg cttttatcag atgaagtttt
     5161 ggaaagaaaa gaagagatac ttgaggctct tttacacctt acaggtggag ctgtatgcta
     5221 acaataaata ttttaattgt acaaataaca tatgaaaaaa attttagtta agaataatta
     5281 aaattctatg tatgatatat gataaatggg gctatctcaa aacacagata cgcctctttt
     5341 tcattgttga aatgataaag tatatttgag ttcatacaaa aaagagtgtc ccatgaatta
     5401 taaaattcat tttgaggcac ccacattatt ttataataat tgcttattaa ttttatttta
     5461 actatataaa atgtacaaaa agtaagaata tccaatattc taaaattaat tttgaattgt
     5521 attgattgaa aagtttacta ttccactatt tccatcttga tttactacta gataccatat
     5581 tccagtatta ggaactctca ttctacatgg atttttcttt gcaaatccac catagtaatc
     5641 ataatttttt tgatttttaa atttcacata atttatatgg tctaacaata ttacattggc
     5701 ttgtttctct aaatttacct ctacaatatc cccttttttt aagtttatct tactataact
     5761 atattccatt gaattactcc tccagtatcg aataatcttt gttatttact catctcaaat
     5821 atataaaaat tcatttaaaa tattacaaat actatataat tagactatta tccagtttta
     5881 accttattta ctacaatcca aaggttatta aaaatattat tggcaacaac ttctccatcc
     5941 catacaactc tttcttcttt attttctaat aaagcatagt acccagagtc agaactttca
     6001 tctttataga ctgtaacata aaactcttca tcactatatt caggtatatt tttaaagtct
     6061 tcagatattt tacatgttaa tatataccct tctaattctt catctataaa caaagcaatt
     6121 cctccaatct ctatattttt actaattata ccatattatg tcataaactt tttattttat
     6181 tacaaatttc tattttttct ttactttata aataaattat ataaattttt tctttttaac
     6241 tttatattac actaattaat tcttttgttt tcttatacat ttctttataa tttataaatt
     6301 tagccattaa tttatacgac tttatatagg taaacatata ctctcactta atattaaata
     6361 atttacattt tttctatact ataatcacat tcttttgtca gttagtaatt ttcaaagtag
     6421 attacttcaa ttaaaatata ataaaattga tttaaaaatt ataataaact aatacctatt
     6481 aaaaatatca taaaaaaaca agtattttat cgaatgtaaa atacttgttt cttataatta
     6541 tttttgattt tttagcatat catttagagc atcatatgtg aaatttatta tttcttcaca
     6601 agttacttta ttttttttaa gagatacaca tatttctgag ctgtatttat ttcttactct
     6661 attcaatagt tctcttgaat acttccttgc tatattttca tctaaattac tatttcgccc
     6721 atttaaatac cctattataa caacagcagc atttacagct ccacaaacac ttccaacatt
     6781 cattccaacc cccataccac ttcctattga gattggtata tctgtgttgt gttcctcatt
     6841 ataagatttt attaatgcct ctgcacaagt gtatccttgg ctatgatata ttgatggtct
     6901 tgtcattttt atacttcctt tcaaatttta ttttatatgt tttgctactt taaacatgct
     6961 aatggtctac atatcatata tactattttt gtatctataa aatccaacct atattcacta
     7021 taaataaatt tataatttta tcaatttata ttacttttta tttataaagt tactttaatt
     7081 taaagcttta cttgatattc ttttattata tattaaatta actctatttt ctgtgactta
     7141 gtcacacaat ctatatataa taatttaatc tactctttta ttgttataca atagaccatt
     7201 atatattagc tttaaatttt taggcactta ctgcacttca ttcaattaag tcgtatatta
     7261 ttatctttaa ttctttgtat ttaaacaaaa gatttgctaa aataaaaata agtagtacta
     7321 cataaattaa acccttagga ggttgtaaaa ttgaaaaaaa tacttataac aggtgcatta
     7381 ggacaaatag gttcagaatt aacaataaag cttagaaatg agtatggaga acaaaatgta
     7441 atcgctagtt caaggagagt aaaagaaggt aatccagtat gtgaatctgg tatatttgag
     7501 atattagatg ttacagataa gaatcgtttt tttgaaattg caaaaaaata tgatgtagat
     7561 actataattc atctagcatc tttactttct gcagttgctg aaagtaaacc tctagaagct
     7621 tggaacttaa atatgaatgg attaataaat ggtcttgaaa tagccaaaga attggattgt
     7681 aaatttttta cacctagttc aattgctgct tttggtgaaa attctccaaa aaatatgact
     7741 cctcaagata cattacaaag acctaatact atgtatggtg tgactaaagt ttctggagaa
     7801 ttactatgtg attattatca ctcaaaattt ggagtagaca ctagaggtgt tagatttcca
     7861 ggtcttattt cttatgttac gcctcctggt ggaggaacta cagattatgc agttgatata
     7921 tattatgagg cattaaaaaa taagagatat aaatcttaca tagcagaagg tacaaaaatg
     7981 gatatgatgt atatgcctga tgcacttcaa tcaatagtag atttaatcga agctccagcg
     8041 gataaactca tccatagaaa tgcttttaat attactgcta tgagcttttc tcctgaagaa
     8101 atagctgatt ctataaaaaa atatattcct gattttgtaa tagaatatga tgtagaccct
     8161 gtaagacaag gtatagctga ttcttggcct aactctctag acagtagtag tgcagtaaaa
     8221 gaatggaact ttaagtttag ttatgattta gataaaatga ctgaagatat gttagaaaaa
     8281 ttatcagaaa aaggaatagg taaatagcaa cctatcattt ttatcaaaat aggcaatata
     8341 ctccatcttc aatgtaagca gggtggagat ataggcctgt aatatgttaa tataagttaa
     8401 aaatgggtaa aaaattatat agctaaagac ttttaatctt aaaatatatc taaataagat
     8461 tcaaaaataa ataatagtat taaaaggaaa ggaaaagcta ctatgtaggt agcaggtact
     8521 aatattgcta aatttaaaag aaattagaaa ggaaataata aaaaaataca taggtgatag
     8581 tttaatttct gaacttacag aggaattaaa aaatatactt ataacagatt tacagtctaa
     8641 cccgtttaaa agtaatattg aactcagtat aataccagca aatatagaaa tatttaaaaa
     8701 tatagaaaat gtattactag actctaaagt taatttaaaa atgggttggg aaagaatact
     8761 tataaattat atcttaaaag atttatttga attaaattat gaaataaata aaagttgtaa
     8821 tgaacctttg atgtttagag tcattatatc tattgattct tatgatgctt aggctcaata
     8881 aataatttac ctgaggatgt gctttagatt tatgaaatac aagttattta tagactttga
     8941 atttaatata ctagacgata ataaaaataa accaaaagaa tataatggtg cggaattaat
     9001 ttctattggt ggagttttag ttgataacga atttaataca ctagataatt attactcttt
     9061 agttaagcca aaatacaata aaatactttc aaataaatgt aaaaatttaa ccaaattgaa
     9121 ccaactggat atcaataatg ctcctaatct tttatatgtt atggatgatt tttataaatg
     9181 gttttgtaaa tttgatgatg ttacaatata caactggggt gattttgata taactggcct
     9241 tctaacttca tttagagtat ataaatatac aggtaaatgc ttagagttat ttaatatgat
     9301 gattgacatt caacctttta tatctcaaca tattacatac aataatagaa tcttatctaa
     9361 gcaaatatct ttactgaata tgaaaaaaat tttttctgta gaaggagata ttaaacataa
     9421 ctcactttca gatgctgtgg atttaatgaa cgtatgcaaa tgctttttct ttaactcccc
     9481 taaaaatatc gatattcttg aagaattata taataaatta cctgctctca aaacaccttt
     9541 atattatatt ccctactttg aagatgaaaa ttttgaactc aaatttgata gaactccaga
     9601 agatataata atatatctta aacaaatatt aactatacta aatataagta aaaaagaaat
     9661 ttattttaaa aaaagaagtg tattagtgaa tgactctaaa attattaact ttaaaaatct
     9721 atcttcttct ttcaaattaa taaaactcaa tgaatttgaa gaatatcctg attttatctt
     9781 aaaattagga gataaaaaga gttttgttga atctgttatt aaaataaata aaagtaacag
     9841 aaaatctata aaaaat
//
LOCUS       DAEMAE010000136         9764 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEMAE010000136 DAEMAE010000000
VERSION     DAEMAE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.138"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            464..1372
                     /gene="trxB"
                     /locus_tag="KPH93_002575"
     CDS             464..1372
                     /gene="trxB"
                     /locus_tag="KPH93_002575"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG1689382.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEEKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARKNIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1465..3288)
                     /gene="typA"
                     /locus_tag="KPH93_002576"
     CDS             complement(1465..3288)
                     /gene="typA"
                     /locus_tag="KPH93_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG1689383.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3500..3742
                     /locus_tag="KPH93_002577"
     CDS             3500..3742
                     /locus_tag="KPH93_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689384.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3890..4081
                     /locus_tag="KPH93_002578"
     CDS             3890..4081
                     /locus_tag="KPH93_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG1689385.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4372..6873
                     /locus_tag="KPH93_002579"
     CDS             4372..6873
                     /locus_tag="KPH93_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG1689386.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQISNGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEIETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGNPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7033..7701
                     /locus_tag="KPH93_002580"
     CDS             7033..7701
                     /locus_tag="KPH93_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689387.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7685..8962
                     /locus_tag="KPH93_002581"
     CDS             7685..8962
                     /locus_tag="KPH93_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG1689388.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDDQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRVNKKNYNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9363..9584
                     /locus_tag="KPH93_002582"
     CDS             9363..9584
                     /locus_tag="KPH93_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689389.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         3890 a         1104 c         1652 g         3118 t
ORIGIN      
        1 aactattaat cataattggt tcaacaaaag tatatagtta ataatgttta atttgaagaa
       61 attgttagtg tgaaattaca tatatattca aattaaattc ataaatattt cacataataa
      121 tagtatactg taaacaaaag gaggtattaa atatgggaat agtacttata acagcagcat
      181 tagttttggt tatgtatgga ctttcaattc tatcatatat atcagaaagc aatatattag
      241 aagacactgc aaatgataac caataaggtt ttgaattata taaataggtg agaactgtaa
      301 ataaaagtta atttttattt gcagtttttt tatatatatg taaaataaag ttaattaata
      361 aaaaataatg ttttgtttat agtatatatg gataataata gtaaagtata agaataatga
      421 gaataatata taagaaataa taatataaag agaggtaaac gttatggtag atatcattgt
      481 aattggtgct ggaccagcag gtttaacttc agcaatttat gctatgagag caggactaag
      541 cgttacagta tttgaaaaaa atatttatgg aggtcaagtt gcaagtacct ctgaggtaga
      601 aaattatcca gcagttcaaa agatttctgg ggtagaattt tctaataata tatacaatca
      661 agcagttgct caaggtgttg atattcagtt tgatgaggtt gaagagataa atttagagga
      721 aaaagtaaag gttgtaaaaa cttcttcagg tgaacataaa gcaaaggctg ttattcttgc
      781 aaatggagtt gaaagacgta aattaggttg tgcaggggaa caagaattta caggtagagg
      841 agtatcttac tgtgcaacat gtgatggagc attttttaaa gataaagaag ttgctatagt
      901 aggtggagga aatactgcac tagaagatgc tttattttta gctaataatt gcacaaaagt
      961 gtatttaatt cacagaagag atagttttag aggtgaagaa gtgttggaga aatctgtgaa
     1021 agcaagaaag aatattgaga tactttacag tcatggtgta gaaaaaatag aaggagaaaa
     1081 gactgtatcg aaaatagaag taaaaaactt aaagactgaa gaaaaaagaa ctatagatgt
     1141 tagtggtatt tttattgcta taggtcttaa acctaataat aaaatgtttg aaaatgttct
     1201 tgatttggat gaaggtggat atataatttc agatgagtct tgtacaacat cagtagaagg
     1261 tgtatatgta gcaggagata gtagaactaa gtttttacgt caaataataa cagcagcatc
     1321 agatggagct attgcagccg ttcaagcagc taattatata aatgttgaat agtataaaaa
     1381 atttcaaata aaaatagcca tataaactga aataattttt cagtactata tggctatttt
     1441 gctaattaga ggtaagtgta tgttttattt accttgtcta gatttgttat atcttaatct
     1501 atcatgttca tttaagaatc tttttctaag tcttatagaa tcaggtgtaa tttcaactaa
     1561 ttcatcatct tctataaatt ctagtgcttc ttcaagagta aatattctag gaggagataa
     1621 ttttatagca tcatctgaac cagaagcacg tacgttactc attttcttgt ttttacaagg
     1681 gttgactacc atatcatctt ttctggaatt cataccaatt atcatacctt cgtaaacttc
     1741 tacaccaggg tctacaaaca ttacagctct atcacttaag gcattaagag aataccccat
     1801 agtaacacca ggtccttgtg ctattaaaac accatttcct cttgaaggta tttctccttt
     1861 aaattcttca aatttttcaa atgaacgaac taaagtacct tctccacgag tagcgtttat
     1921 aaactcactt cgatatccta aaagaccacg agtaggtgct aagaactcta tttttacata
     1981 gtctccttct attgacatag actccatcat accttttctc atgtttaatt catttataat
     2041 agtaccggag taaacttcag gacagtttac aacaactctt tctattggtt ccattagttt
     2101 tccatcttct ttatgcatta aaacttcagg tttagaaaca ccaacttcat aaccttcacg
     2161 tctcatattt tcaagtagta tagataagtg aagctctcca cgtccagaaa ctttgtatcc
     2221 atcagtagta tcaagaggtt caactttaag accaacattt acttcaagtt ccttttcaag
     2281 tctatctttc aaatgtctcg ttgtaacgaa ttttccactt tttccaacga atggagagtc
     2341 atttactaag aagttcatag aaagagtagg ctcttctatt tttatcattt ccataggtag
     2401 tggactatct aaatcgcata tagtctcacc tatagatata tcaggaatac cagctataac
     2461 aactatttct ccactagtag cttcatcaac ttcaacttgt tttaaacctt catacacaga
     2521 aagttttgat acttttcctt tagatacaac agaatcttct ctacatatag caacttgtgt
     2581 attgttttta agagtacctt tgtaaattct acctatacca agtctaccta cataatcatc
     2641 atatgcaaga gctgatattt ggaattgtag aggttcatta tcgtaattag gataagcttc
     2701 aacgtgattt acaatagttt taaatagagg agaaaggtcc tcactatcat catccatttc
     2761 aagtttagca ataccttgtt tagcaatacc atatattata ggaaattcgc attgctcatc
     2821 tgttgcattt aagtctacaa ataaatcgaa aacttcatta actacttctt ccgctctctg
     2881 gtctttttta tcaatcttgt ttataaataa tattggtttt aaaccgaact ctaaagattt
     2941 ttgtaaaacg aatctagttt gaggcattgg gccttcacta gcatcaacta atagaataac
     3001 tgtgtcaaca gttttcataa cacgttcaac ttcagaagaa aagtcactgt gtcctggagt
     3061 atcaactatg tttattttgt aatcttcata gtttattgca cagttttttg aatatatagt
     3121 tatgcctctt tctttttcaa ggtcgttact gtccataacg cagtctttaa ctacttcgtt
     3181 ctttctaaaa acaccacttt gagaaaggaa agcatcaacc aatgttgatt ttccagcgtc
     3241 aacgtgggct ataactgcga tatttataat tttatgtttt tgtgacattt ttgttgctcc
     3301 cttcgattaa aataacttat taattatagc acaatatatt attaggtaca aggaggaata
     3361 tattaaattg tattaatata gtttaaattg gataaaactg ttagttttac atctgaaaat
     3421 tattctgttt aaaaaaattt tttagacaaa taataacctt aatagataaa aagattaata
     3481 aaagagtaaa ggatgatata tgttggaagt aaccaaaata ataattaaat attttgcaag
     3541 tgaaagtgat acttttgtaa aagaagaagt ttgtaatttt gaagaacttc ttaagtggtc
     3601 tcataaattt attgaaggta aaaaaataga agatgttttg tatataaagt ctaaagaaaa
     3661 ctataaagta aatgtgatta agtctttaga aattaaggag ttggaaatag tgagaaaaca
     3721 acagatacca ccatacatgt agtattaata atatataaaa ttaatatgta ataatcacaa
     3781 taggatataa atggtatata tggtaataat ttatattaaa aaaactttta taaagtttat
     3841 atttgggtat aaacgtatta attagacatt aattaggagg aacttaaata tgataagagt
     3901 atgtccaaac tgttcaaatg tggatgtaaa taagctaaaa actttggttg gagaagaaaa
     3961 tgtaaagact ggttgtatag gtcagtgtag agcatttaaa aaagaagctg ttggaataat
     4021 agacaatgag cttgaaataa aggaaacaga agcagagttt tttgaagcat gcaaagaata
     4081 aattttaaat aaaatataaa ttataataaa tagagcatat aaattgtata agtattaaac
     4141 aatttatatg ctctattttt atatatacat ttaattatga gtatactttt ctattaaata
     4201 caaataaata taactaatac atttatttat taaaaaaata ttgatttaat atacctatag
     4261 ggggtataat aaaaatatag atttctaaat aagtaattta tcaatattac atatcaaaat
     4321 aaaacgttac aaattagcta aaaatatatt gaaagaaagg aagattttat tatgagttca
     4381 aataaagtgg tagaaagtta taaaattaca ggtatgacat gtgcagcttg tgcaaaggca
     4441 gtagaaagag taactaagaa gatggatgga gtatatgacc aaagtgtaaa tatagctact
     4501 gaaaaactta aaatagaata tgataattct aaggttaatt ttgatgatat aaagcaggtt
     4561 gtagaaaaag caggatatgg aattataaag gaagaaagta ataaaaaaat tgatatgaaa
     4621 attgatggaa tgacatgtgc agcttgtgca aaagctgtag agagagtggt aaaaaaattg
     4681 gatggagtag aaagtattag tgtgaatatt gcaacagata aagctaatat agactatgac
     4741 ccatcaaaag taaaattatc tcaaataaaa gcagctattg aaaaagctgg atataaacca
     4801 atagaagagg taagaaataa ggttgatgtg gatgaagata aattgagaaa agaaagagaa
     4861 atgaagtctt tatttgttaa atttatagtt gcaatagtat ttgcagtacc tctgttttat
     4921 atagcaatgg gacctatgat aataaagcct atagggccat ggcctttgcc agaaattata
     4981 aatcccatga caaatacttt taattacgca ttaattcagt taattttagt aataccagta
     5041 atgatagctg gttataaatt ttatataaat gggtttaaat cacttttttc tttaagccct
     5101 aatatggatt ctttagttgc aataggaaca ttagcagcat ttttatatag tctatatacg
     5161 acactacaaa tatccaatgg acaaattcaa gggatgcacc atcatcagtt gtattatgaa
     5221 agtgcaggaa taataatagc acttatctta cttggtaaat atttagaatc aaaatcaaaa
     5281 ggaaaaacat cagaggcgat taaaaagctt atggggctac aacctaaaac tgcaatagtg
     5341 ttagttgatg gtaaagaaat tgaaactcct atagaagaag tagagatagg agatatacta
     5401 ttagtaaaac caggaactaa aatacccgtg gatggagttg taatagaagg atatacatca
     5461 gttgatgaat caatgcttac aggtgaaagt ataccagtgg aaaagaatgt tggaagtaag
     5521 gtgacaggag ctagtattaa taaaaatggt gttattaagt ttaaggctga aaaaattggt
     5581 ggagatactg ctcttgctca aataataaag ttagttgaag atgcgcaagg tacaaaagca
     5641 cctattgcta aacttgctga tactgtatct ggatattttg taccaatagt aattgcaata
     5701 gctgtagttg catcattatt gtggttttta ataggaggaa aagatatagt atttgtactt
     5761 actatattta tatcagtact tgtaattgct tgtccttgtg cattaggatt agcaacacca
     5821 actgctataa tggttgggac tggaaagggc gcagaaaatg gaatcttgat aaaaggtgga
     5881 gaggcactag aatctgcaca taaagtaaat acagtgatat ttgataaaac aggtactata
     5941 acagaaggaa atccaaaagt aacagatata gtgttaaata ataatgtaaa agaagaatat
     6001 cttatcaaga ttgcttcaag tgcagaaaaa ggttcagaac atcctcttgg agaagcaata
     6061 gttaagtatg gagaagagaa gaatataaaa tttgaaaaag ttgataattt taaagctata
     6121 ccaggtgctg gtatacaagt tactattaat gatgaaagta tattacttgg aaatagaaag
     6181 cttatgaatg acaataatat aaagcttggg gatttagaag aaaaatcaaa tgtattggca
     6241 agccaaggta aaacacctat gtatatagct gtagatggaa atttatcagg tataatagca
     6301 gttgcagatg tagtcaaaga aagtagtaaa aaagcaatag aaatacttca tgatatggga
     6361 ataaaagttg caatggtaac tggagataat gcgaaaactg caaatgctat agcaaatcaa
     6421 gtaggtatag atatggtttt ggcagaggtt ttaccagaag ataagtcaaa agaagttgaa
     6481 aaactacaaa atcaaggcaa gtttgttgca atggtagggg atggaataaa tgatgcccca
     6541 gctcttgcaa aagcagatat agggatagca ataggaagtg gaacagatgt agccattgaa
     6601 tctgcagata tagtacttat gaagagtgat ttaattgatg ttccaacagc aattaaactt
     6661 agtcatgaaa ctataaaaaa tataaaacaa aacctatttt gggcatttgg atataatacg
     6721 attggtatac ctgtagcagc tggaatatta tatgtatttg gaggaccttt attaaaccca
     6781 atgattgcag ctgcagcaat gagcttaagt tcagtttcag tcgtatcaaa tgcacttaga
     6841 cttaaaaact tcaaggcata taagagagat tagattaata aattaaataa aatgtatcaa
     6901 aaagagctat ctttaaatta aaaggattaa tttacaaatt aagatagctc ttttttcatg
     6961 taaaattcac aacaataaga taaaatataa gtaatatatg aaattttaaa taaaatagaa
     7021 aggattgata gaatgaattc gtcaatactt gtaattgaag atgattcaaa tatacaagag
     7081 ttaatatcag aatttttaag tgcagaaggt tatcaagttg atacagcaaa tgatgggttg
     7141 gaaggtatac aaaagtttaa gcaaggaagt tatgatttag taatattaga tataatgatg
     7201 ccaaacttag atggttatgg agtttgtaaa atgataagaa agtcatcaag cgttccaatt
     7261 atatttttaa ctgctttaaa tgatgaagga gaccaactta aagggtttga cttagaatgt
     7321 gatgattata taacaaaacc attttcgttt aatcttctta taaaaagagt agaagcaatt
     7381 ttaaggcgaa gtaacaagac tataaatgat aaatttatag tttttgaaaa attaaaatta
     7441 gatttaaaca catatattgc agagatagat ggagaaccta tagaacttac actaaaagag
     7501 tttaatatat taaaagcttt gatagaaaag tatccacaag ttataactag agaaggtctt
     7561 ttggatagta tatggggtta tgactattat ggagatacta ggattgttga tgcacatata
     7621 aaaaatataa gaaaaaaaat atcattacca tatattaaaa ctgtaaaagg aatagggtat
     7681 acacttgaga aggatatttg ataagtggga aaagttaagt ataaagtata agttattttc
     7741 aataacgaca tcattattaa tagcattagc tttaataatc tatctaattt tatatttttt
     7801 attgccatca tattatcatg agtataaaat tgagtctctt caagaaagtt tgaaatcttt
     7861 agtagatagt tcaattcatt tcgatacata tactttagaa gaaagacttt attatatggc
     7921 taaagaccaa aatttggcta ttttattaaa agatgaccaa gggaaaattg tatatggtaa
     7981 gaatgaagta gttattttaa gatatagtaa gtacatgatt aatagtcttg aggatgagta
     8041 tagaacttct atacctatat atactaaaga tgcaaaagat ggtccatata ctttagaact
     8101 cgttatgcca ttacaaccaa ttgacgaagc aaacgaagtt ataagaaaac taatgccata
     8161 catcatatca atagcaatat tgatagcgat tattggagca tatatatatt caattgtaat
     8221 aacaaagcct cttataaata taatagagag tgagagagaa caggaatata gaaggaagga
     8281 ttttgttgct acaatatcac atgaactaaa aactccaata accataataa gtggtcaaat
     8341 tgaaggtatg atttacagtg taggtaagta taaggatagg gatacttatc tgaaaaaatc
     8401 gtatgaatgt acacaagaat taaaagattt agtaaatgaa atgatagagg tttcaaaatc
     8461 agaaatactt gaaaaagatt tgaaattagt atcaataaat ataagtgaac tattaaatag
     8521 acttgtaaaa agacaagtat ttttaataga agaaaaacat atgaaaacta ttttaaaaat
     8581 agaagaaaat ctagaaatta aagcagacca agagaggata actaaggcaa tcaataatat
     8641 aataaataat gctataaaat attctcctga agaatcggag cttataatta gactctatga
     8701 taaaaataaa cgagtaaata aaaagaatta taatcaaaga gtagttttag aaattgaaaa
     8761 tactggtgta actattgaga aacgttattt agaagagata tttaatccat tttatagaat
     8821 agaaaaatct cgctcaagaa aaactggagg aagtggactg ggactttata tagtcagcca
     8881 aatttttaaa agtcatgggt ttgattatag cataaaaaac aaagaaaact ctgtagtatt
     8941 cacagtagaa tttaaaaatt aattacttat tgtaaagaat atataacttt caataatagt
     9001 tcattttaaa atcacaaaaa attcataatg aaactgtatt atataaatat aaagaagctt
     9061 taaaccataa aatacactag ctataatgat gagatttcct taaatcctcc atataagtca
     9121 tcattatagt aacccaacaa ttaagttact atgaatttaa aaaataacac caagcatata
     9181 atctatgctt agtgttattt ttattttgaa ataaaaaata tttaatttat tgaaatatat
     9241 agatttagat agtttaaact atatttaaga cttaaaaatt ataattgata ttacaaataa
     9301 atatatgtac tttgggaaaa ataaatatag gaagtattat atttacagag gaggtagttt
     9361 tgatggatag attaaaaaga gatatgaaaa atatgaaaga agatttaaaa gaaggtgtta
     9421 aaaagacagc ttatgttatg aaaaatacag ctgaagattt gaaagatgat gcacaaggtg
     9481 ctatggtaaa cttagaaatg aaaaaagatg agatgatgga aagatatgaa caaaagaaat
     9541 tttctaaaga attagagaag caaatgaaga atgaaatgaa ataagctttt atttatgaat
     9601 aacaaattat tgtattcata taaaaagtag ccagtatatt aaatagttta tttaaggaaa
     9661 tagactttct aaaaataact gtattagatt tagaaagtct atttcaaatt agttttatga
     9721 atagacataa ggagaaagta gatgttatag aactaaattt taaa
//
LOCUS       DAEMAE010000137         9687 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEMAE010000137 DAEMAE010000000
VERSION     DAEMAE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.139"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      55..98
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            199..1167
                     /locus_tag="KPH93_002583"
     CDS             199..1167
                     /locus_tag="KPH93_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG1689390.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFANLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQERIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1169..1732
                     /locus_tag="KPH93_002584"
     CDS             1169..1732
                     /locus_tag="KPH93_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1689391.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFITQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKISLTGGK"
     gene            1740..2429
                     /locus_tag="KPH93_002585"
     CDS             1740..2429
                     /locus_tag="KPH93_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1689392.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2556..3284
                     /locus_tag="KPH93_002586"
     CDS             2556..3284
                     /locus_tag="KPH93_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG1689393.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKKFLEVAEDTYYEEVRLQGMIIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKNYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKTLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3298..3972
                     /locus_tag="KPH93_002587"
     CDS             3298..3972
                     /locus_tag="KPH93_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1689394.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDAGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            3998..4834
                     /locus_tag="KPH93_002588"
     CDS             3998..4834
                     /locus_tag="KPH93_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689395.1"
                     /translation="MNKIVKKSISLMIILTIFIFTLTACEKDEQPDSVDIQAEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            4850..5521
                     /locus_tag="KPH93_002589"
     CDS             4850..5521
                     /locus_tag="KPH93_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689396.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FLLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5511..6743
                     /locus_tag="KPH93_002590"
     CDS             5511..6743
                     /locus_tag="KPH93_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689397.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKNPKDLVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKKFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKSGDEIVLELREDIEDEV
                     ILLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIEINVT
                     SDIDIGTTFELSIPKKLK"
     gene            6893..7210
                     /gene="trxA"
                     /locus_tag="KPH93_002591"
     CDS             6893..7210
                     /gene="trxA"
                     /locus_tag="KPH93_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG1689398.1"
                     /translation="MAKVINTSEFRSSVEGSKGIVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7227..8105
                     /locus_tag="KPH93_002592"
     CDS             7227..8105
                     /locus_tag="KPH93_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1689399.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            8375..8743
                     /locus_tag="KPH93_002593"
     CDS             8375..8743
                     /locus_tag="KPH93_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1689400.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            8746..9117
                     /locus_tag="KPH93_002594"
     CDS             8746..9117
                     /locus_tag="KPH93_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG1689401.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(9253..>9687)
                     /locus_tag="KPH93_002595"
     CDS             complement(9253..>9687)
                     /locus_tag="KPH93_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689402.1"
                     /translation="GDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEISYTYNINA
                     KDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEVTRKVNSI
                     IDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
BASE COUNT         3962 a          934 c         1673 g         3118 t
ORIGIN      
        1 cagtctatag tttttaagaa tctatagtaa gtagcctaaa agtatgattt tacatctgta
       61 cttgttcgag aactccaagt ataaaaaact aaggaattat atagtggcag tatataatta
      121 atacccttag acacaccttt ttgaaagtgg aaaaaagatg tgtagttctc ctcgaccaca
      181 aatcaaggag gataaaaaat ggaaaagaga aaagtaataa ttgattgtga cccaggaatt
      241 gatgattctt tggcaattct tctggcttta aactcaccag agctagaagt aattggaatt
      301 actacatgct gtggaaatgt tccagcaaat ataggtgcag aaaatgcact aaaaacactt
      361 caaatgtgtt cttcactaaa tattccagta tatataggag aagaagcacc actaaaaaga
      421 aaacttgtaa cagcccaaga tacacacgga gaagatggta ttggagaaaa cttttatcaa
      481 aaggttgtag gagctaaagc aaaaaatgga gcagtggatt ttataataaa tactttacat
      541 aatcatgaaa aagtatcaat aatagcactt gcaccactta caaacatagc taaagcactt
      601 attaaagata agaaagcatt tgcaaatctc gatgagtttg tatctatggg aggagcattt
      661 aggattcatg gaaattgctc tccagtagca gagtttaatt attgggtaga cccacatgga
      721 gcagattatg tttacaagaa tttatctaaa aaaattcaca tggtaggttt agatgtaact
      781 agaaaaattg tacttactcc taatattatt gagtttataa atagacttga taagaagatg
      841 gcaaagtata taactgaaat aactagattt tatattgatt tccattggga acaggagaga
      901 ataattggct gtgtgataaa tgaccctcta gcagtagcat actttataga tagaagtata
      961 tgtaaaggat ttgaatcata cgtagaagtt gtagaagatg gaatagctat gggtcagtct
     1021 atagtggatt ctttcaattt ctataaaaaa aatcctaatg caattgttct aaatgaagtt
     1081 gatgagaaga aatttatgta catgttttta aagaggcttt ttaaaggtta tgaagacatt
     1141 atagactctg tggaaggagt gatatagtgt gaaaagaaaa atatgtgtaa aaactattac
     1201 acttatagct tttggtattt ccataaatat aataggcgct tttatagcta tgggattaag
     1261 attacctgta tatttagatt caataggaac tataatgata gcttcacttt taggacctaa
     1321 atacgctgtt gttacaggtg tgtttggaag tttaataagt ggaattacgt ttgatgtgta
     1381 ttctctctat tttgcaccag ttcaaatatc aaccggatta cttgctggaa tagcatttaa
     1441 aaagaatttt ttaagaggtt taaaaactcc actaggagta cttttatttg ctattccaac
     1501 atctataata agttcaataa tttcagcatt tttatttgga ggtatgacct cttcaggctc
     1561 atcctatata gtgcaaatct tgaaagtgtt aggactggga gatgttttta gtgtattcat
     1621 aacccaagta tttactgatt atggagataa gctgttagca gttgttttag tgaatttagg
     1681 attaaatgca gttccaaaaa ctttaaagat atcattaact ggaggaaaat agatagaaaa
     1741 tggatagata tagtgagata acaaataaaa atcaaagaga aattgttctg cttaaaggat
     1801 ttccttgtat atggggaaaa tgttcttttt gtgattatat agatgataat tctaatttag
     1861 aagaagagat gaataaatta aatctaaagg ttttaaaaaa cgtaactggg aaatatggag
     1921 ttcttgaagt cataaattct ggaagttgtt ttgaattacc taaggatact ttagaaaaaa
     1981 taaaatgtat tataaaagag aagaatataa aaaaattatt tttagaaagt cattggtctt
     2041 acaaaaatag gcttaaggaa atgagagagt attttgaaat tcctgtggta tttaaaattg
     2101 gagttgaaac ctttgacaat gattttagaa ataatatttt aaataaaaat gcaaatttta
     2161 agactccaca agatgtaaaa gaatattttg attcaccttg tattatggtt ggaataaaag
     2221 gacaaacaaa agagatgata gataaagata tagagattat cttaaatact tttgaaaaag
     2281 cgacagtgaa tgtttttata aataattctt ctagtataaa aagagatgaa gagttagtga
     2341 aatggtttgt tagtaagtat aaattcttgg atgaaaatcc aaatatagaa gttttataca
     2401 ataatacaga ttttggtgta ggagattagt gttaatttag tttaaattta aaattgaaaa
     2461 gtttcttatg tttttgaaaa agcttctaat agttagaagc tttttttgta gagttttatg
     2521 ttacaataag aaaaaaggaa ttaaggagta attaaatgat gtttgatagg ggaaaatgga
     2581 ataaattgga ttataatgaa cttttagaat atatgatgtc tatacaagat ataaaatata
     2641 gagattttaa taaaaagctt atacctggaa cagaaaatat aataggtatt agagttccta
     2701 atttaagaaa gttatcaaaa gaaataagtc aagggaattg gaagaaattt ttagaagtag
     2761 cagaggatac atactatgaa gaagttaggc tacaaggtat gataattggc aatataaata
     2821 gtaattttga agaaacttta tactatgtaa aaagatttat acctaaaatc gataattggt
     2881 cagtttgtga tggattttgt tctggtttaa agtctatgaa gaaatataaa gaaaatatgt
     2941 atgatatact taaaaattat gtttattcaa agaatccatg ggaaattaga tttgcattag
     3001 ttatgttttt aatatattat gttgatgata aacatattaa agaaatattt gaatactgta
     3061 ataatataca aagtgaagaa tattatgtta aaatgggtat ggcatggttg ctatcaattt
     3121 gttttattaa atgtgaagag gaaacttttc tctatataaa aaataataat ttagatgatt
     3181 tcacttataa taaaactcta caaaaaataa ttgaatcaaa tagagtagat ttagaaaaga
     3241 agaatataat aagaagtatg aaaagaaaga caagaagatg ttgacggagg aatattaatg
     3301 aataacaatg aagacagaat tgacaaattt ttagaacttc aaagcaaagg atttaaattt
     3361 gcagagatag ctcaaaatct aaagattagt caaagtactt taagaacttt tttaaataaa
     3421 aaaggatata agtcagaaaa aggtaaatac atattaaaag acgaattaaa ttataataaa
     3481 agtttagata atagaaactt ggaagaagaa aaagctaaag agtatgaaaa aagtaaaaag
     3541 aatactataa gtaatagaaa aaaagtaagc accaaaaaaa agatttctaa tgaaaaggct
     3601 ttaagtaaaa gtgttgaaca aattgagttt aatgatgcag gtttaaaaaa tgcaaagcca
     3661 aaacaaaatg ttaagcctaa gaaagataga aaaataaata taactcaaga agatttagat
     3721 aagctttgtg aagtatatga ttggtattta gaagtaaaag attacaatat aaagcctaaa
     3781 aaagtatcta ataaaaaaga tgtacatata gatgatgaac acatagatga attaaaaaca
     3841 acaagaatac gtatagataa agaaatatgg aatgattttg aaagattatg tagtaattcg
     3901 aattttgaaa aacatcaaat acttacacag gcattgaaag aatttatgaa aaagtataaa
     3961 aatctactat aaaatactac tatatgaggt gcttaatatg aataaaatag taaaaaaatc
     4021 aatttctcta atgattatat taacaatatt tatttttacg ttgacagcat gtgaaaaaga
     4081 tgaacagcct gattcagttg atattcaagc agaagataaa aatgagataa aaatagatga
     4141 gggaaatgca aaagtattga atataggaaa atcagaaatt ataaatatag atgaaggtga
     4201 taaagtagat acatcaacaa aggtagaaaa taattcaaca tttaatatat ccaatgttga
     4261 attgatatat aatgaatatg atgagaataa aaaaattata tcttcagata gtaaggcttt
     4321 gttggacatg actttaatgc ctgggaaagt tgcttacata gaatgtgggc ataaaacttt
     4381 tgtaaagagt gtagaagttt atgcatatga atatgaggca gagggtaaga ttgtgtatgt
     4441 taatttaaaa gaaaatacta ttaatataag aaataataat attaagctag aaaatagtag
     4501 tcagtatgag gtgctatcta catcagaact taaaaaagta aatgaatcaa atgagggaat
     4561 tacgtatcaa gtaaaagtaa aaaattcatc atcaaaagat ttaggaaata ttattcttaa
     4621 gacagcagaa gttaatgaaa atggagaata tttaacagta aatcgtgtgc catcatataa
     4681 gattttaaag ccttcagaag agacagatat agatatattg tgctcaacaa aggcaaaaag
     4741 tgttgaaata gtgggatata cgtatgatga tataaaagaa aaagccaatg tggatattga
     4801 cttaaaatct cataaagtta agatagacaa ataaaggtag gggatactta tgaaggtttt
     4861 aatagttgaa gataataaga tacttttaga aagtgtagta gaagaactta gcaagcattt
     4921 tgaaactgaa aagtgtgaag atggagaaga agctttatac ttagtaaatc aaaatatata
     4981 tgatttagtt attttagact taatgcttcc taatataaat ggatttgata tcctaaagaa
     5041 aatgagagtt aataatatag atacacctgt acttatattg acagcaaaag aaacattaga
     5101 tgacaaagta gaagcattta ctataggagc taatgattat ttgacaaaac ctttttatat
     5161 ggaagagtta gtagcaaggg tatacgctat attaaggact aatggaaaga taaaagaaag
     5221 aaacggatta gaatttaaat ctttatattt agatacacta gaaaaaagag tgtatattga
     5281 aaaagaagaa ataaaacttc aaaacaaaca gtttaacttg cttgagtatt ttctattaaa
     5341 taaaggttca attttattaa aagagcagat ttatgataga atatggggaa ttgattcaga
     5401 tgcaactatt gaaatagtgg aagtatatgt aagtaatcta agaaaaaagc taagtaaata
     5461 tggatatgat aaatatatta aaactaaacg taaggtagga tatatattcg atgataaata
     5521 agaatgtatt taccagtact aaaaatcatt taataaaaat gtatattatt gtagtaggta
     5581 gttttttaat aatcttctct atatttatat actcatattt taggggactt acatatagtg
     5641 gaattgatag tgagataaat gatgaacttg aatatatagt ttctcaattt aaaagaactt
     5701 catttttgaa cccaataaga ctcaaaaacc ctaaagattt ggtgtatgta tatgaagatg
     5761 gaaggataag ttactatact caaaatgaat attttgatga gttgcttcca gatagaagac
     5821 tagataaaaa aaattcgttt tttaaataca cagaaaatgg atatactttt agagaattaa
     5881 atgtagatgt aggtagatac caaatacaaa taattagaaa tatagattcc gagatgaatt
     5941 ctttaagaca gcttacatct gttcttataa ttggaatatt aatttctgta ataattactt
     6001 actttgtggc tgtgtatctt actagaaaag ctttaattcc aatagaaaca gcatggaaaa
     6061 atcaagctaa gtttatacaa gatgcttctc atgaattaag aactccaata acaatagttt
     6121 catcaaaatt agaaagtatg ttaaaatcac cagaaagtac agtaaatgat gaagttgaaa
     6181 caatagctac tgcaatgaag gaaactagaa gacttaaaaa aatgataact gatttattgt
     6241 ctcttactaa agaggactct attgttaaag ttaatttaga agaaatagac ttagaaaagt
     6301 tattggaaga aatatcagaa gactatattg acattgctga gtttcaggaa aaaaaattcg
     6361 tgtttaattc taaactgaag aataaagtta taataacaga taaaaataaa ttaagacagc
     6421 ttatattgat tttcattgat aatgctttta aatatacaaa atcgggtgat gagatagtat
     6481 tagaattaag agaagatata gaagatgagg taattttact tataagtgat actggtatag
     6541 gcataaaaaa agaagaaata cctcttatat ttgatagatt ttttagaagc gaaaatgtaa
     6601 gaaataaaga tttagaaggt tctggaatag gactttcaat agctcgtatg attagcttaa
     6661 acttatctat tgagataaat gtaacttctg atatagatat tggaactaca tttgagttgt
     6721 caataccgaa aaagttaaaa taataaaaat ataaaaaatt gtttttagaa atgaagaatg
     6781 ggtatactaa ttagtataaa atataaaaag accttataaa agtaaattta tatttataaa
     6841 tttcatagta taattatata gggataataa agtttaatag gaggatttaa aaatggcaaa
     6901 agttataaat acatctgaat ttagaagtag tgttgaaggt agtaagggaa tagttgtagt
     6961 tgatttcttt gcaacttggt gtggaccttg taacatgttg ggacctgtat ttgcagaatt
     7021 aggtgaagag atgaaagata aagctagatt tgtaaaggtt gatatagatg agagcttaga
     7081 aatagctcaa caattcaatg tatctacagt accaactatg attatattta aagatggaaa
     7141 accagtagaa actttaattg gattcatgcc aaagaacaaa attgaaatgc aagtaaaatc
     7201 atatttataa aaaatgggtg agtattatga gatatgatat agctataatc ggcagtggtc
     7261 cagctggatt gtctgctgct ataaatgcaa aaataagaaa taaaacaatt atcatgtttg
     7321 gtaatgataa tttaagtaat aagcttgtta aagccccttc aatagataat tatttaggct
     7381 tttatgatat aagtggtgat gaattaaaag acaagtttaa atcacatata gatagtatgg
     7441 atatttcaat tgaaaataaa agaataaata atatttatgc tatgggagaa tactttacaa
     7501 taatgtctgg aaatgatatg tatgaagcta ctactgtaat ccttgcaact ggggtagagt
     7561 atactagacc aatcaagggt gaagaagaat ttctaggaag aggtgtaggc tattgtgcta
     7621 catgtgatgc accactttat agaaataaaa aggtagcagt tataggatat aatgaagaat
     7681 ctaaagaaga agcaaatttt ttaagtgaac tcacttcaaa gacttatttt atacctatgt
     7741 acaaaaaaga taatcttatg agaagttcag ataacctaga tgattctatt gaagttatac
     7801 atgacagacc agtacagata gatggagata aacttgtaaa caaagtatct tttaaagaaa
     7861 accacattga agttgatgga gtattcgtta taaaagatag tacggctcca agtgcattgg
     7921 ttcctggaat agagattgat ggcattcata ttaaagttga caataatatg aagactagta
     7981 tagatggatg ctttgctgct ggagattgtg ttggaaaacc ctattcttat ataaaagcag
     8041 ctggtcaagg gcaaatagca gctttaaatg ctgtttacta tttagataaa ttaaagaggg
     8101 cttaatattt atattgatta actttttaaa gatatttata aaaaactgtc ttgaaataat
     8161 tttattattt caagacagtt ttttattttt aaattattaa taaaagtatt ttaaaatcta
     8221 tatgtaatta cttttattaa aaaaattcaa agttatatta ggaaaaattg ggattattta
     8281 taatataccc caaaatttaa aaataatggt tgaaaaatct aattaagagt actataattt
     8341 aattaagtaa ttacttaatc aaaacgaggt acttatggaa aatttggtta aaatatttaa
     8401 agcattgagt gatgaaacta gacttaacat acttatatta gtatccaaga gaaatatatg
     8461 tcaaaagggg atttctaaat atctagggat ttcagattct gctgtttcac aacatataaa
     8521 aatattaaaa gatgttggga taataacagg atataaagaa ggatactatg ttttatatca
     8581 tattaataaa gaatcattta atatatgtgt agattttata aattctatgt taagtaattc
     8641 tagtgaaagc tttatagatg tatttgatgt gaatacaata catctaggat gcagtaaaga
     8701 atgtaaatct ataaaaaaat gttgtaagag aagggagaaa taataatgaa agtatgtata
     8761 ccagtagaag agaataaagg attggatagc aaaccatatg gacattttgg ttctgctcca
     8821 atttttgttg tgtgtgattt agaaagtgga gaagttaaat ctttagataa tggagattta
     8881 gatcatgaac atggaaaatg tcaaccatta aaagcattgt cagggacagc tgtagatgca
     8941 gtagttgttg gaggaatagg acaaggagct ataattaaat taaatggaat gggaataaaa
     9001 gtttacagag cagaaggtga tactataagt gctaatttag acctacttaa aaatggaaaa
     9061 ttagtagagt ttccatcaga ccatacttgc tcacatgatg gatgtggaca tcattaaaaa
     9121 aaacagctga tgaaatatat gcttcatcag ctattttttc ttttaataaa tacaatttta
     9181 ttcttttaaa accaaaattt agatttttat gttttttgta gtatccttga taatatccca
     9241 agtttactac ctttagattt tttttgtatg tttttctata tagtcaatat catttaatat
     9301 gtcattaagt tccttattaa gtgtttttaa atcatcacca tctacattgt agtttaaaaa
     9361 tagttcctct acattatcaa tgatagaatt gacttttcta gtaacttcaa cgaatgtttt
     9421 aaagttacta ctatttttag catttagaaa tttttttaaa tcctttgata taaatgatac
     9481 tatttcatat tcatcattaa atagacctct accaatagga tatggttcaa ttctctgtaa
     9541 aaaatattgg tctttagcat taatattata agtataagat atttcccaac cgtctcctaa
     9601 atcaaacttg ataaaatata atgtactact tacttttgat attcttgtgg caccaagttg
     9661 tcttaaaaga acttcaatcg aatcacc
//
LOCUS       DAEMAE010000138         9635 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEMAE010000138 DAEMAE010000000
VERSION     DAEMAE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.140"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            38..763
                     /locus_tag="KPH93_002596"
     CDS             38..763
                     /locus_tag="KPH93_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG1689403.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     DSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGRESFKSARAYADENFIYIVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(891..2087)
                     /locus_tag="KPH93_002597"
     CDS             complement(891..2087)
                     /locus_tag="KPH93_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689404.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGNLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIVKEALKLDKKVYSFRNRDESKKYYDHLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(2090..3115)
                     /locus_tag="KPH93_002598"
     CDS             complement(2090..3115)
                     /locus_tag="KPH93_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1689405.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGMLGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLVFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPVVVMLL
                     VKRKDRA"
     gene            complement(3187..4317)
                     /locus_tag="KPH93_002599"
     CDS             complement(3187..4317)
                     /locus_tag="KPH93_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1689406.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKNDKENKQASSGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKNLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGFNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLYEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(4545..4725)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4778..5671)
                     /locus_tag="KPH93_002600"
     CDS             complement(4778..5671)
                     /locus_tag="KPH93_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG1689407.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVISHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAKIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMDKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(5751..6653)
                     /locus_tag="KPH93_002601"
     CDS             complement(5751..6653)
                     /locus_tag="KPH93_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG1689408.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNISSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(6703..7863)
                     /locus_tag="KPH93_002602"
     CDS             complement(6703..7863)
                     /locus_tag="KPH93_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1689409.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQINKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTEDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(8018..8323)
                     /locus_tag="KPH93_002603"
     CDS             complement(8018..8323)
                     /locus_tag="KPH93_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1689410.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSKISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(8421..9395)
                     /locus_tag="KPH93_002604"
     CDS             complement(8421..9395)
                     /locus_tag="KPH93_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG1689411.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTIIA"
BASE COUNT         3123 a         1768 c         1055 g         3689 t
ORIGIN      
        1 ctacatatat aaaaataaat ttatgaaggt gattttaatg aaaactattt ctaaacaaga
       61 attagaaaaa acacttgaga aacattattt atggcttaaa aacaataatg ctggagaaaa
      121 ggcagattta aaagaattaa gattagaaaa tatggactta agaggatact gcttaagtaa
      181 tattgacttt tcgtggtctg accttatcaa tttaaattta gaaaaagcag atttagaaaa
      241 ttctattttt aataactctt atctttcgga ttgttcatta aaaaattcta tattaaaaaa
      301 tgcagactta agtggtgcaa attttagatt ttgtgactta tctgactcag atattagagg
      361 agctaattta gaaaattcca atttagaaca tgctacttta aatggagtaa tatcagatga
      421 aagtactcgt ttttttaaat tgtattgtcc tgaaacagga gcatttatag gctataaaaa
      481 atgttttaat tttagaatgg tacaattatt aatacccagt gacgcaaaaa gagtgtctgc
      541 tacatcaaat gcttgtagat gtgataaggc aaaagttctg tcaattacta gtatagatgg
      601 tagagaatct tttaaaagtg ctcgtgcata tgctgatgaa aattttatat atatagtagg
      661 agaaacgatt gtagctgaag attttaatga aaatagatgg aaagaatcga ctacagggat
      721 tcactttttt ttaactagag aggaagctat tggttatcta taaaatgcta aagttttata
      781 aatatataaa tacataaaca aaataacaaa agagtatgga ttgtaatttc aaaaaaatta
      841 taatctatac tcttttttct atagcacata agtacatttt atttcataaa ttataataaa
      901 ttatttatat ctgcactatt aattatttga gaaactttat ctatatgttc tatcttgcta
      961 tccaagtggt cataatactt cttgctctca tctctatttc taaaagaata aactttttta
     1021 tccaatttta aagcctcttt tacaatatct atattcctct tgtttgtttc tcctacacta
     1081 aatcctgtat ctattataat cttagaatca tttaaatttc taatagccaa atcgaaactc
     1141 tcatcactta taggctcaaa tggattttct gtaaacatct ttatccccat agttctacca
     1201 atttcataat caatatcatt ttcatgtatt atcccagaat acagacctat acctttttta
     1261 gtaaaagctc tataaattgg tgttgcttta cccccaccac caattataaa aacttcattt
     1321 ttagatttat tactaatttc tactgaaccc aaaagactat taaaattttt atcgtccaat
     1381 tcataaagtg aatttataat atcctcatct actacatctt caggttgacc ataagctatg
     1441 actttattgt tttttataag agcaacctta tcacaacttt ttaatgctat gtctatttca
     1501 tgtaatgaaa gtattactga tattgacttt tctttactta attttttcag tatgtttata
     1561 agttccaatc tatgttttat atccaagtga gttgtaggct catctaatat aattatttct
     1621 ggttcttgaa cgagagctct tgcaaccaac accttttgtt tttctccatc acttagttca
     1681 tcaaaatatc ttttctttaa atgtaaggca tctacagact ccaatgcttc atctaccatt
     1741 attaaatctt tcttagatag attcccaaaa aatcctgtat atggatacct tcctatattg
     1801 actatatctt gtacagtcat taaatcacct aataatctat ttgtaagtac taaagccatt
     1861 ttttttgata atgcttttct tgatatacaa tttatgttta caccattcaa atatatttca
     1921 ccttttattg gtgatataag tttagataag gaccttaata gggtagtttt tccagctcca
     1981 ttacttccca aaagacatag tatttctcca ttttttactt ctacatttat attacttata
     2041 actaccttgt tattgtatcc tacacttaaa ttattagttt tcaacatgtc taagccctat
     2101 ccttcctttt caccaataac attactacaa ctggagctcc taataaagat gttatcgtac
     2161 ttattggtaa ttctactggt gataaaatta ctcttgatat taaatcacaa agagctgtaa
     2221 ctatactgcc taaaaatatt actactggta tcaaaactct attgtctgat gttttaaata
     2281 ccaattttga aatttgtggc actgcaagtc caataaaccc tataggtcct gcaaatgctg
     2341 atactattgc tgccaatata ctagatataa atactatcaa aactctaaaa aatcttatat
     2401 ttatgccaat actttttgca tactcttctc ctaataaaaa agcatttaaa ggttttatta
     2461 tgaatacagt catcaaaaga cctaacatac caactattat aagtatttct accttttccc
     2521 atgtaaatcc agaaaaactt cccatagacc acatagtaaa ttcttttaat ttatcactat
     2581 ttgcaaatga ttgtaacaca tttgttatgc cactacatac atatccagtc ataattccta
     2641 ttattaaaag agttgttata ttctttacct tatttgcaaa taaaatgact atagacatta
     2701 caattaaagc tcctataaat gctgctacaa atatactcat tgaatttaca ctttcaaatc
     2761 ccaatgtgta tcctcccaaa atcatgagag ctactgataa agtagaacct gaagatactc
     2821 ctaatatata cggttcaact attgggtttt taaaaaatat ctgtaataaa atacctgata
     2881 ctgctaggca tgctccacct accatggttg caagtactct agggattctt atattgtata
     2941 ttattttata atgttctgta ttatccatac ctcttaatac aacatctatt acttctttaa
     3001 attcaatttt tacagagcca aatgctacac acactaaaaa agtaagtatc atacttataa
     3061 ttaatactat aaatattgtc gtattcctta ttctcatttt attgataaaa tccatttgta
     3121 tactcctctt agcaaagcta tattaactaa aatctataaa acctatcttc ttgtgtcctc
     3181 tctgtactat ttatcataat ttatataatg tttgattttt tcacctgtag tacctgggtg
     3241 gaacatctca actaaatcta ctattaattc atccgtctta tctactgatt gataataatc
     3301 tggagcaaac acccataggt ttccgttttt tacaacatcc aaatcagcca atacaggtgc
     3361 actagatttt atagaatcta aatttggtgc ataatctgaa cttgatgagt atacaaatat
     3421 atctgcatct tttgcaactt catacaattg ttcaatactt actcgccctt catttgaatc
     3481 tattgaagca catgcattta ttccaccagc catttttatc atattatcta catatgaatc
     3541 tttaggagct atataagcac ttccttcata tacagatgcc catacaactt taggtttagc
     3601 tttgtttttt atttttttac ttacttcttc taccttttgt acagttttat ttagttcact
     3661 agtagccata tcttctttat cataaaatgc agctattaaa cttgtccact ccattcgtcc
     3721 aaatggattt tgctctttat attcattatc tacaacataa ttaaacccta attcctttaa
     3781 cttatcaata aattttgtat cagcttctga taagccacta ctcactatag ttaagtcagg
     3841 atttaaatct actatttttt cataatctgg tgctaaattt gaaccaacgt aatgtatttt
     3901 tccatctttc attaaatttt taatttcttt aatagtccag tactgctctt ctgttgttac
     3961 tgcttttata ctatctagtt catctattgt tctcagagaa caagcctgtg ttgtagatgc
     4021 tattaataca ttgtccacag gcgttctaac tatattagca tttttatatt tttcaggaat
     4081 tttaatcttc tttggaatta aaatcagttc tcgattatct ccatcagtca cttttttaat
     4141 tccatcttct aaatcttcaa cactaaatcc actagcatat tttaattcta atttcttacc
     4201 actactagct tgtttattct ccttgtcatt tttactacac cctataagac ctcctaccat
     4261 aaaaattacc atagcaatta aaaaaaactg ctttaaaaca tttgatttaa atttcatctc
     4321 tttcactcca atatcttatt ttttatgagt ggctaaaaaa ctttaataat atatgctttt
     4381 taaagtatac ttattaaagt tatcctaaag tcatctttaa taagtataac cttttaataa
     4441 aataaaaaac ttctcctaat ttaataagga gaagttcata tatcaacttt acaaaaacga
     4501 attgatactt cattttatta taaattacaa tctccaccct aaattgaact tatataatac
     4561 tgggcaggtc tcctgactca cggcttttcc tccatcatcc ttcccaaaat ttttataatt
     4621 tcagtggatt ttgatttcgt atccgtatac agtagcgggg gctgtagtga attttcatca
     4681 ctttccctat taaactcata tagtacccta gtattatttt aatttaattt ttattaagat
     4741 aattgtaatt tattgttgac ctctttgcaa ggttgattta acatatattt aattcattca
     4801 tcaatttttt aatttcacat acttgttttt catttggttt tacaaatgga gcagtcatat
     4861 aatcgcttat ttgtagtcct ctgatattca tagctttttt cattgctggt ataaatggat
     4921 tggttattaa gtaaaaatcc attgctttat taatatattt ttgaatttct ataaccttgt
     4981 ccatatctct attattgtaa gcttcagccc atttggagca aacttctgga attaaatttg
     5041 atagtccccc tatacaacct gctcccccag acattagatt atgtataaag ttttcatcat
     5101 aaccactata aaccttaaat tctgggaact ctattttcat agtatttatt aaatctctgg
     5161 tatgtcccat caaagttact gtatccttat atccaactat gtttttatgt tttcttgcaa
     5221 gtcttaatgt aagttctgga cttaaatcat aaccagttct atctggataa ttgtataaat
     5281 atattttagc ctctgtattc ttagctattt cactataaaa taactcaata ctatcatcat
     5341 caagagagaa ataataaggg cttattatca taactccatc tgctccacta ttatgtgcat
     5401 agtttgaaag ttctatagat tcatttatac acatacgact tgtacctata aacactttta
     5461 ctctcttatt tacatgacta attactaaat ctataagttc tttttgcata tccatagaca
     5521 tattaaagaa ctctccagta cttcccatta tgactagtcc atctacacct ccatctataa
     5581 gatggtcgta cactttttta ttaccttctt tatctaaatt tccttcttca tcaaatattg
     5641 taacaactgg tgtcaaaaat tctgctttca taatttaaac tcctaaaatt tattttaata
     5701 tgttcattaa tacatcagat ttagctttaa tattaattat attaaaacat ttacttaatg
     5761 taatttttta aaattccaat actctcaatc tcacattcaa ctacatctcc actactcata
     5821 tatcgaggag gtgaaaatcc catccctact ccagaaggtg ttccagtagc tataatgtct
     5881 ccagcctcta aagtcatacc ttttgaaagt tctgaaacta tgtctgaaac gtctgatatg
     5941 aataatcctg tattagaact ttgtctttct tcaccattta ccctactaca tactttaagt
     6001 tttatgggaa aaggaactgc acttttatgt aaaatacatg gacctaaaga ggtaaatgta
     6061 tctaatcctt ttcctctaaa ccattgaaca tgttcttttt gcaactttct agcagaaata
     6121 tcatttataa cagtgtaacc aaagatataa tcttctacct cttcttttct aatattaaca
     6181 cctgttttac ctattactac tccaagttca acttcatagt ctagttgatt atttatctca
     6241 aaatgaccat ctatctcatc ttctggacct attgcctttg tcacacgttt tgtaaaataa
     6301 accgtttttt gtggttcttc aaaactatca tcaaagtgag tttgagtttc ttcaagatgg
     6361 tctttatagt tcactcccac acataagata tcatgtattg gtttttctat aggaacatgt
     6421 acagtacatc tctctctttt aaaaacatca taattataga atgtactttc atttataata
     6481 gttttcaact taataatatc ttcgctagat atattttgaa ttaattcatt taaatcctta
     6541 aaatttctac taagatttaa agagttaaca tctataataa ataattcatc tttagaaaaa
     6601 actccagttc ttttacattc attttctcct tcagtaaaac ttacaaactt cataatctca
     6661 ccctttatta aaattctatg tatttatttt cttacttaaa gattatacat aattgcatac
     6721 tctagctatt gccatttcag tatatccaag agactctgct tttgattgtt ttccatttgc
     6781 tacatctaca accatattta acaaatcctc tgttaattct tccattgttt gtggaccata
     6841 tataactgga ctagcatcaa aatccatatt atcttccata tttgcaaaag ttattttatt
     6901 tccagttatt tttattactg gagctattgg atttcctgat ggtgttcctc ttcctgtact
     6961 aaataccact atttgtgctc ctcctgctac cataccagca acagatgaag catcatttcc
     7021 aggggtatcc attataacaa gacctttctt attaatttgt ttggcataat catatacttc
     7081 acttatttgt ctatgaccac ctttatgtat acaaccaagt gatttttctt ctaaacttgt
     7141 taaaccacca gctatatttc caggagatgg attaccttct ctaacttctt ctccaaccaa
     7201 ttttaatgaa ttttcatatc tatgtactat atgtaatatt ttttctttta cttcttcatt
     7261 tactgctctc ctagctagta tatgctctgc acctataaat tcagtagttt cacttaaaat
     7321 tgatgttgct cctaaatcga caagtttatc acttaattca cctattaatg gatttgctgc
     7381 taatccactt gttgggtcag acccaccaca ttcagttcct agtataagtt cagatattgg
     7441 aaattcttct tttctaagta aagaagcttc ttgtgccatt tctctagcat aacgaacagc
     7501 tttttctata gtttttaaag ttccatgttc ttcttgtatt acaagagttt ttattggctt
     7561 atttgttctc tcttttattg catcaactac caaatcattt tggcaatttt cgcatcccaa
     7621 agatacaact attacaccat atacatttgg atttgctgca taaccagcca taacatccat
     7681 agttaattgt tggtctgaat gtacttgtga acatccattc tgattattaa aggtaactgc
     7741 tccttgaact tgtgatgcta ttattctagt tgtatctgat gcacacacac tcgtaggcaa
     7801 tattaatatg tggtttctta taccaactct tccatctggt cttttatatc cataaaattt
     7861 catactgatt tcctcctaaa atagtctata tacttttata attttcatat attataattt
     7921 ttattacact cactaatcaa acagctaaaa tcctatttaa aaactatttg acactaaaag
     7981 caatgaatac aacttttata actctataaa tattatttta tatattagtc ataacttgtt
     8041 tatccaaatc ctctctaaca cttctaacat tatgaatatg tacatgttga ccacttttta
     8101 tttcctgatt tgcttctcct atatgttcac catatttagt aactttatca ccttgggcca
     8161 tatcttttat agccaattta tgataaatag ttatatcatc tagtgctgta atcgttttta
     8221 ctgttttgtc ttttaattta aaactaattt tactaccttt agctattgct tcaatagcta
     8281 ctgcaacatt atctttttca tcaattatca ctgcatttat cattattctt gctctccttt
     8341 ataaataata taattttatt aatatcctaa aatctaatta aagaatttat tattttacca
     8401 acaactcact gtaatataaa ttaagctatt attgtaccat cctcattttg ttttggaaac
     8461 ttaggacatc catacttctt agcccaccaa ctagttataa gaggtactaa tattgcagtg
     8521 acaactactg cggctgccac ttgtgttgta gctgttgcaa catatggttg ccatgatggg
     8581 tcaactaatg caacagcagc aggtacagca attgcatttc cagcagttgt agctacagcc
     8641 catcctgcat atcctggacg ttttcctata aatttatcac agaatactat aaatatacct
     8701 cctacaaata cagatattaa tccaagtaat actcctgata aacctccttt tagtatactt
     8761 gcaagattta tgctactacc aagtgcaaac cctacaaaag gtacgagtaa attacaccct
     8821 ggctctaaaa actctttcat ttttttgtca aaatttccta ttatcatacc aacaagtatt
     8881 ggcactattg cagccaataa tgacaacaaa ggtacatttg caagacctga agtcccaaga
     8941 gctattaatg taaaaaatgg tccatcattt aaagtaagta aactcatagt tgcacaatct
     9001 gtttcatctc catatgaagt catcaatgaa agatatatac taccattact atttgtaact
     9061 gcacttataa cagccaatgt tgtaagacca aggacaccat ccattccaaa taactttcct
     9121 ataagtatac ctatcaaagc acctatagca aatttagaac caagaagtat accacctctt
     9181 tttaaaactt ttggcatttc tctaaattga agctgagttc ccaagcatac cagttgaacc
     9241 ccaattatag ttgcagaccc aacacttgaa aatactgctg tagtaaacga acctatttgt
     9301 acaatatctg ggcaaaaggt attaatgaaa gctgctataa acattggtac tatcatcata
     9361 cctgcaggaa tttttttaat aaaatccatt atcataaagt taatcctcct aaaacatgat
     9421 atttaattac tcttattaag tttttagctt taattctaac ggccgtttta gttcgtattt
     9481 aagaactagg tttttaatta cgaatctttg aattaatgat agcattttcc tatttatgag
     9541 tcaataaaaa aaataaattt ttgtcaaaat tttttcaaac tttctgataa tacattgtat
     9601 attatcaaat ggcttcctta tatgcaaaaa tacaa
//
LOCUS       DAEMAE010000139         9616 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEMAE010000139 DAEMAE010000000
VERSION     DAEMAE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.141"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            69..1259
                     /locus_tag="KPH93_002605"
     CDS             69..1259
                     /locus_tag="KPH93_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1689412.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMLKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTT
                     FENL"
     misc_binding    1393..1648
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1707..2708
                     /locus_tag="KPH93_002606"
     CDS             1707..2708
                     /locus_tag="KPH93_002606"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG1689413.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            2812..3201
                     /locus_tag="KPH93_002607"
     CDS             2812..3201
                     /locus_tag="KPH93_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG1689414.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVITKKDKHSNIIENVEFSIKDEEE"
     gene            3362..4570
                     /locus_tag="KPH93_002608"
     CDS             3362..4570
                     /locus_tag="KPH93_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG1689415.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(4657..5733)
                     /locus_tag="KPH93_002609"
     CDS             complement(4657..5733)
                     /locus_tag="KPH93_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1689416.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            5962..6489
                     /gene="hpt"
                     /locus_tag="KPH93_002610"
     CDS             5962..6489
                     /gene="hpt"
                     /locus_tag="KPH93_002610"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG1689417.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            6578..7510
                     /locus_tag="KPH93_002611"
     CDS             6578..7510
                     /locus_tag="KPH93_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG1689418.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVIF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKFFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            7572..8798
                     /locus_tag="KPH93_002612"
     CDS             7572..8798
                     /locus_tag="KPH93_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1689419.1"
                     /translation="MKKVVIIGDGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            8882..9103
                     /locus_tag="KPH93_002613"
     CDS             8882..9103
                     /locus_tag="KPH93_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG1689420.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            9292..9462
                     /locus_tag="KPH93_002614"
     CDS             9292..9462
                     /locus_tag="KPH93_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689421.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
BASE COUNT         3770 a          992 c         1678 g         3176 t
ORIGIN      
        1 aataatatta aattgcatct agtaatggac ttaacaatta taaaaatttt attcttagga
       61 gggaaaaaat gagtaagtat aattttgaca gagtaataga tagagtagga acagattgtg
      121 taaaatggga ttttagaact aattgttcaa caaaagcaca aaaagatggt ttgccttttt
      181 ggattgctga tatggatttt gaatgtgcag aaccaataat agaagcatta cgtaaaaaag
      241 ttgaacataa aatatttgga tatagttcaa atgatagtga taaatacttt aatgcagttt
      301 gtgattggta taaaaggaga tttaactggg aaataaatag aaaagatatt atttttagtc
      361 ctggtgttgt acctgctgtt gctattttag taagaatatt aactaattct aatgaaggtg
      421 taatagtcca aaaaccagtg tattacccat ttgaagctaa gataaagagc aataataggg
      481 acgttgtaaa caatcctcta atatatgaga atggtactta taggatggat tatgatgatt
      541 tagaagaaaa agctaagtgt agcaacaata aagtactgat actttgtagt cctcacaatc
      601 ctgttggaag agtttggaga gaagatgaat taaaaaaggt tgtagaaata tgtaaaaaat
      661 atgacctgtg gataatagct gatgagatac attctgattt aattagaaaa ggatttaaac
      721 atactccttt acagtcatta tgcccagagt acaaggataa aatagttaca tgtatagctc
      781 caagtaaaac atttaatctg gcaggaatgc agttgtctaa tataatagtg aataatgatg
      841 aactaaaaaa gaaatatcaa gaagaggtta cagctgttgg agttgcaact tcaccaaatc
      901 cctttgctat tgtagctaca atagcagcat ataatgaaag tgaagactgg cttaatgaat
      961 taaatgatta cttagataat aatatacaat ttattgatga atacttaaaa gaaaacctac
     1021 caaaagtaaa acttgtttat ccagaaggaa catatctagc atggttagat tttactgctt
     1081 atggattaaa tgaagtagaa cttgaggatt taatgcttaa aaaagctaat gtattatttg
     1141 atgaaggtta tatatttgga aaagaaggaa taggatttga aagaataaat gtagcttgtc
     1201 cacagtcttt attaaaagaa tgtatggaca gattaaagac tacatttgaa aatttataaa
     1261 gtataggtaa aaatttataa agtgtttaaa aataaaaaaa taataaactt gacaaatttt
     1321 aactaatcac ttatcatact attatgaata aatagaaaac ataagagagc taaattttat
     1381 tgaattaaac tcttaaagac tttgaaagga agagtagtta tagaatgtaa ttaaagcgag
     1441 ttggtgatgg tgtgagacca atatgaagtc tatagtgaag aacatcctgg agttgctaac
     1501 cgaaatttta aaagagtagg cttagtcgga accaaaccgt tatcatattg gggtgtatcg
     1561 agtaatatct gtacattgtg agctgggttt gaatttattt caaactaatc agggtggtac
     1621 cgcggaaaag caacctttcg tcccttttat atagggatga agggtttttt taatggaaaa
     1681 atatataaat atagggagtg aatgatatga gcacaataat accaaaggat tataaaagta
     1741 gtttgaacgt aattgataca caaatagcta taaaaaaatt aaaagatttt tttgaaatga
     1801 gactaagtaa tgaattaaat cttataagag tttcttctcc gttatttgta ttaccagaaa
     1861 caggtgcaaa tgataattta aatggagaaa aggctgtaag ctttgatata ccatttatga
     1921 ataaaaatgc agaaattgtt caatcacttg caaaatggaa aagattagcc cttaaaaaat
     1981 atgggtttga agttggtagt ggtttatata cagatatgaa tgcaataaga aaaaatgaag
     2041 aattagacaa catacattct ctttatgtag accaatggga ttgggaattg gttatagata
     2101 aaagtagtag aaatgaaaag actctaaaag atgttgtaaa aagattgtat ggagtattta
     2161 aagatactga aatttttgta tgtagtatgt atgaaggtat aaaagaaata ttaccagaag
     2221 aaataacttt tataacatca caagagttag aagatatgta tccagaatta tcaccaaagg
     2281 aaagggaaga taaaattgta aaagagaaga aagcagtatt tataactcaa ataggaaaaa
     2341 cattaatatc aggagaaaaa catgatggaa gaagccctga ttatgatgat tgggaattga
     2401 atggggatat attattttac aatcctgttt tagattcagc attagagtta tcttcaatgg
     2461 gaattagagt tgatgaagaa tctcttgata gacaattgaa acttgctgga tgtgaagaga
     2521 gaaaagagtt tgactatcat aagatgcttt taaatggcga acttccatat acaattggtg
     2581 gaggaattgg tcagtcaaga atatgtatgt atttcttaca aaaagctcat ataggagaag
     2641 tacaagttgg agtgtggcca caggatatga ttcaaaattg tagtagtgca ggaattgaat
     2701 tattataatt taaatataaa ctaattgata aaatttatat ctaatatgtt catcaaatta
     2761 attatatcgg ttataatgta gaataaccta taattataag aggtgataaa tatggagttt
     2821 ctaaaaataa tcattaatat agttttagat attttaaaaa aaattttggt gagatttaaa
     2881 aatgctaaat ttggtttggt ttttgtattt gatttactta aactacctga ttttatgaca
     2941 gataaaagaa tcaatatagt tgataaaata aaggtaattt ctgtattaat ttttactata
     3001 tcttactttg tatctggggt tgatataatt ccagagatga ttgcaggggc atttggtttt
     3061 atagatgatg ctatagtttt aatttggagt ataggtatag tgaatgaaga aattaataag
     3121 tatagagtta tcacaaaaaa agataaacat tcaaatataa ttgaaaatgt agaattttct
     3181 ataaaagatg aagaagaata acaatttata tatgttattc ttttttttat taacaaaaaa
     3241 tggttaaatt gttgacggag aggtaaaact ctattatcat aataagtgga tatccaatat
     3301 ttaactaagt attcaatgtg caagaatgct taatttaaaa aagttatgga gggtaaatat
     3361 tatgaagaaa aaaataggtt tagtgcccaa actaatcatc ggtatcatag tcggtatttt
     3421 aatcggaagt tatgcaccag aaataatagt acaaatatta gtaacagtta gtactttatt
     3481 ctcagcattc ctaaaatttg ttataccatt tataattata ggttttgtaa cagcaggtat
     3541 agctgattta gcaacaggtg caggaaagtt attggggata actacgggaa tagcatatgg
     3601 ttctacttta atagcaggat tattatcatt tgcagtatct acattaatct tcccaaattt
     3661 tattgatgca agtgttgcaa gtcaaattgg agacccagag gcaggaatgt tagctccaat
     3721 attcacaatt ccattagagc ctatggtaga tgtaacagct gcaatcgtat ttgcatttgt
     3781 aatgggactt ggaatatcag ctttaagaaa tcatggaaaa ggagagactt tatttaattt
     3841 cttccaagaa tttcaaggaa tcgtaacgaa aacgttgtca acagtgatta tacctttatt
     3901 acctttatac atagcaggaa cttttgcaaa cataactata gcaggggaag tatggacaat
     3961 attaggggta ttctggaaag tatacttagt agtaatacct ttacattttg tatatatggc
     4021 atgtcaattc acagcagcag gtgtattttc aggaaaaaat ccagtaagaa tgttaaaaaa
     4081 tcaagtacct ggatatttaa cagctatagg aactcaatct tcagcagcaa ctataccagt
     4141 taatgttggt tgtgcagaaa aaaatggagt aagtaagcaa ataagagaat ttgttgtacc
     4201 tctttgtgca acaatacatt tatcaggtag tatgatatca ataacaggat ttacaactgc
     4261 agttttaatg atgaatggta tgccacatgg aataggtgta ttattccctt atattgcaat
     4321 gttaggtata gcaatggttg cagcaccagg agcaccaggt ggggctataa tgagtgcact
     4381 tcctttccta ccaatggttg gaataccatc agatggtggg cttgcaagtt taatgatagc
     4441 tttatactta actcaagaca gttttggtac agcatgtaat gtatcaggag ataatgcaat
     4501 agcagtagta gtagataaaa taaatgatgg aatgagtaaa aaaggaaaca aagaagagca
     4561 aagtgcttag ttttttaata atcaaaaatg agttatctca aatagagata gctcattttt
     4621 tatggtataa aaaaattgta aaataaagtg atttttttat ttaaaaattg ctttaagaga
     4681 tgatattaat aatttatttt cattaggttt taatatacat actctaaaga aatactcatc
     4741 tagaccttca aatgaacaac aatctcttat tattattttt tgtggaagca gttgttctcg
     4801 aagagattta gcagtcaatt cctttgtctt gattttacac aatatgaaat tacctttagt
     4861 attgtatata tctaagcttt ttatattctt aagctcttta agcaggtaat ctctttcagt
     4921 gttcattaag gaaatagtat ttgatataaa atctaaatca ctaaacatga tttcccccat
     4981 tttagaagct attatgttta tattccataa atctagattt ttatttattt catttttgac
     5041 attagtgtta gatataaggc catatccaag ccttattcca ggcgttgaaa aaaattttga
     5101 tgtacctctt ataacgaata aattagaata atcatcaact aattgagtac aagagtatgt
     5161 atctgtatca gtaaactcca cataagtctc atcaatcatt aaaaatgagt ctgtattttt
     5221 taaaatttct ctcacttcta ctttagtaaa tgcaaatcca gttggattac taggattaca
     5281 gattactacc aaatcataat ctttagcatt gatagttttt ataagatttt ctaaatttat
     5341 atgaaaatta tcctcttctt tggcaaagta tttttctata gaacaattaa ttttagataa
     5401 ctctttttca tactctgaat aggctggaga gagtaaaaga gcctgtttag gattaattgt
     5461 atgtataaat gatgaaatta gttctgtagc tccactgcca agtactatat tatctataga
     5521 acatttgcag taattggata tagaagtttt taagtctatg taatcagggt caggatacat
     5581 agataccata tctatattgt ttacaatgta ttgtttggct aaacttgatg taccaaaagg
     5641 atttatattt gagctaaaat ccataaattc ttcctttgaa aagccatact tactagataa
     5701 actatataaa ttagctccat gatttgtgct cataaaagta cctccaaaaa taaaattata
     5761 taatcgataa aaataactga ttaatatatg tacatataga ttataataca aaggaatcgt
     5821 tattccaaat ttttaatgga aaaagcaaaa aagtatggat aagctgttaa tttaggttta
     5881 aaaatgatga aaaagatttt aattgatatt ttggtatgct ataatatata agttaataga
     5941 aggaaagcgg gaggatacaa aatgtacaaa gttacaggaa aaatgttgac agaagaacaa
     6001 ataaaagaaa aagtttatga attaggaaaa aagatagaag aagactttaa aggcgaagat
     6061 ttactagtgg taggtatact taaaggagct agtgtatttg tttcagattt aataagatgt
     6121 atagatttgg atgtaaatat agattttatg agtgttacaa gttatggaaa ctcaacagag
     6181 tcttctggaa cagttaaaat attaaaagat ttagatgtag atattgaagg aaagaatgtg
     6241 ttgattgtag aggatataat agattctggt ttaacattaa gtaatctagt tgcagcttta
     6301 aaaactagaa atccaaaatc tttaaaacta tgtactctac ttgacaaacc tcagagaaga
     6361 aaagctaata tacctgtaga ctatgttgga tttgttatag aagacaaatt tattgttggt
     6421 tatggaatag actatgctga aaagtataga aatttacctt atataggtat agttgaggat
     6481 gtagaataga tttaaatgtg aatcaaaagc actaatttct aagggagtta gtgcttttta
     6541 gttaattata taaaatttta gtaaaggaga catctatatg gatattcagt taaatatatt
     6601 attatttttc caaaatttaa gaaatccaat tttgaacttt atatttcttg tctttacaat
     6661 aagcacagaa gcaccgttat taatattaat tacaacaatt atatactggt gtataaataa
     6721 aaaatgtgga cagaaaatac tgtttgctat aattggaaat tttgttgtaa atttaggcat
     6781 caaagagttt gtaaaagctc ccagaccaat aggaataaaa ggtcttgaat cattgagagt
     6841 ttctactgct ggtggttatt cttttccaag tgctcatact caaactgcaa caacattttg
     6901 ggttagtatt atgactatat ttaaaaagag atatcttcat ttaataggtg cagttatggt
     6961 tttaggagta ggattgtcta ggctgtattt agctgttcat tggccaatag atgtgatagc
     7021 gggttggata ttaggaatat tttttacagt tatatttgta aaaatatttg actatattga
     7081 tgataataaa aattatattt tacttgtagt tttacttata ccatttataa tagtagctat
     7141 atttctaaat agccctgaat attttgaaaa atttggtata atagtaggat ttgtatttgg
     7201 atatatggtt gaggatagat ttgttaaatt taatacagat aataataaca aaaaaataaa
     7261 ttttagtaat aaaaataagg ggagtaaaaa tataattttt tcaagaatat gtaggttttt
     7321 ggttgggata gtaacaatag gaatgttata tattggcatt aagtttttta tttcaatgtt
     7381 aatagtcact ttaaatataa gtgactctac tatgagtata atttttatga attttattaa
     7441 gaatacagta gttgtttttt atggaatagc aggagctcca gcattattta aacttttaaa
     7501 attaaattga ttttaaggta tagagtcaat agtttaaatt aaaaaaatta agggtagaaa
     7561 agagtgatta tttgaagaag gtagtaataa taggtgatgg accagcaggg atgatagctg
     7621 catcaactgc atgtgagaaa ggctatgatg taacattaat tgagaaaaat cataaattag
     7681 gaaaaaaact tgcaataaca ggaaaaggta gatgtaatat tactaacgct tgtgaaatag
     7741 aggaattaat agaaaatgta cctacaaatg gaaaatttct ttatagtgct ttttatactt
     7801 ttacaaatga tgatgtaata agtatgttta ataatcttgg agtaaagact aaaacagaaa
     7861 gaggaaaaag ggtttttcca gagagtgata aagcatttga tattgtaaat gctcttgaaa
     7921 gacaacttaa aagtaagaaa gtaaatatat tattaaattc gaaagtagaa aaaataatat
     7981 ctaaaaataa taaaattgag aaagtaattt taaacgataa aaaagaaatt aagtgtgatt
     8041 cagttgtagt agcaactggt ggattaagtt atccacttac tggttctact ggtgatggat
     8101 ataaatttgc aatttcacag gggcacacaa ttatagatac aaaaccatct ttaattggta
     8161 tagaagtaca agaaagtttt acaaaagatt tagaaaaatt atcattgaga aatgttgaaa
     8221 taagggtgtt taatagcaaa caaaagaaag tttatagtga ttttggagaa cttgaattta
     8281 ctaaatttgg tttagatgga cctataatca agtctgcaag ttgtagaatg aaagatacaa
     8341 ggaaagaaaa ttacactata ttattagatt taaaacctgc attagatgag gaaaaattgg
     8401 ataaaagagt tcaaaaagat tttcagaagt atacaaataa aaaatttgaa aaagcattag
     8461 atgatttact tccaaaaaaa ctaataccta taataataaa cttaagtgaa attaatccaa
     8521 atacggttgt tcaccaaatt tctagagagc agagaaaaaa cttagtccat ttattaaaaa
     8581 atttaaagtt tacagtaaag agatacagac ctattgaaga agctataata acttctggtg
     8641 gtgtaaaagt aaacgaaata aattcaagca caatggaatc aaaattagta gaaggtttat
     8701 tttttgctgg agaagtaata gatatagatg cgtatacagg tggattcaat ttgcaaatag
     8761 ctttttctac tggatatttg gcaggtttta attgctaaaa attacctgaa ttaaaaatgt
     8821 ttaaaatcac ataatattat cagcaacaca aaataaatta aacagaagga aggtaatcaa
     8881 catggcaagt aacaataaca acaacagaac agtagtacca ggagcaaaag aggctttaaa
     8941 ccaaatgaaa ttagaaatag ctaatgaact tggtatgagt aattatcaac aagttgataa
     9001 aggaaacctt actgcaagag aaaatggtta tgtaggtgga tacatgacta aaaaattagt
     9061 tgaaatggct gaacaacaaa tggctggaaa aagcaacaga tagttataaa taattattta
     9121 taactattgg ctattagttt agctagagct atctcaaata tagaaagtaa tttctaaatt
     9181 gagatagctt ttttttatta aattattaaa tagatatatt tttatgtaat aaattttatg
     9241 tatttggata aaataataca aatgaatatg ttacataagg agtgatgtaa aatgagtagt
     9301 gtaaattttg aggatttaaa aaacaaattt attaatagtg acttagatga aaaaataaga
     9361 atatatacaa caacagaagg tttatcagta gaacaattta aagagctact aaaatattat
     9421 ccaatacaac atttatctaa attagaaaaa gcacttggat agcagtattt agtccaaaat
     9481 taaaaaggca atttatttaa tattaaaaat attttttatt aaataaattg cctttttata
     9541 ataggtttaa attattatgc ttttgatata taattgtaga ttctctcatt tccaagttca
     9601 atagacccag tattaa
//
LOCUS       DAEMAE010000140         9596 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEMAE010000140 DAEMAE010000000
VERSION     DAEMAE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.142"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(82..1281)
                     /locus_tag="KPH93_002615"
     CDS             complement(82..1281)
                     /locus_tag="KPH93_002615"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG1689422.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGALKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     misc_binding    complement(1534..1741)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1852..2589)
                     /gene="truA"
                     /locus_tag="KPH93_002616"
     CDS             complement(1852..2589)
                     /gene="truA"
                     /locus_tag="KPH93_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG1689423.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     gene            complement(2611..4044)
                     /locus_tag="KPH93_002617"
     CDS             complement(2611..4044)
                     /locus_tag="KPH93_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689424.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            complement(4047..4745)
                     /locus_tag="KPH93_002618"
     CDS             complement(4047..4745)
                     /locus_tag="KPH93_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689425.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            complement(4774..6162)
                     /locus_tag="KPH93_002619"
     CDS             complement(4774..6162)
                     /locus_tag="KPH93_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689426.1"
                     /translation="MKVLKFITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMYIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MEIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNRNTYSSKSSANIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            complement(6298..7446)
                     /locus_tag="KPH93_002620"
     CDS             complement(6298..7446)
                     /locus_tag="KPH93_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG1689427.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            complement(7501..8319)
                     /gene="larE"
                     /locus_tag="KPH93_002621"
     CDS             complement(7501..8319)
                     /gene="larE"
                     /locus_tag="KPH93_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG1689428.1"
                     /translation="MEVSFVKEKEKLDKLKKRLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLQDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            complement(8589..9290)
                     /locus_tag="KPH93_002622"
     CDS             complement(8589..9290)
                     /locus_tag="KPH93_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG1689429.1"
                     /translation="MGIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYRHFRPIMNVDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT         3064 a         1726 c          902 g         3904 t
ORIGIN      
        1 aatttaataa attcaaataa aaaaaaagag catataaaaa cttaaaaatc gtttttatac
       61 actcttaaat aatttatttt attattttcc agctttcata gctctgattt ttaatggtaa
      121 agtgaataaa ttaataaatc cttctgcatc tttgtggtca taaagttcac tatttccaaa
      181 agaagaaatt ccttcatcat aaagagcatt ttcagtaaat attccagcag gttttatgtt
      241 tcctttgtaa agtttaactt ttactgttcc tgtaacatta tcttgagttt gttccataaa
      301 tgcatctata gattctctta atttacaata ccataatcca ttgtaaataa gttctccgta
      361 tttataagat accatttgtt tgaaatgtaa tgtatctttg tctaaagtag cactttctaa
      421 aatattatgt gcttcataaa ggattgttcc tcctggagtc tcatatatac ctcttgattt
      481 cataccaaca agtctattct caactatatc tataacacca attccatgtt tacaacccaa
      541 ctcatttaac ttatgaatca gtgcaactgg agataattct tcgccattta cctttttagg
      601 tacacctttt tcaaagtaaa cttctacata ttcagctaca tcacatgcat cttctgggtc
      661 tacacattgt ttgtaaacat cttttttatg ttcattttct aggtgctcta tatccccacc
      721 ctctgttgac acatgccaaa ggttagcatc tactgaatat attttagctt tagttgctgt
      781 tacctttata ttgtgttttt cagcatagtc tatagcatct tctcttgatt ttatatccca
      841 aattctccaa ggtgcaataa cttttatagt tgggtcaaga gctgcaattg tagcttcaaa
      901 tctaacttga tcattacctt ttcctgtaca tccatgacat atatattttg caccttcttt
      961 atgagcaact tcaactaatt ttttagctat tataggtctt gctaaagaag ttcctaataa
     1021 gtattttcct tcatatttag cctctgcttt tacggcttta aatatagttt cagttacaaa
     1081 ttcttcactt atatcatcca catatgcttt taatgctcca ctttgtaaag ctttttcata
     1141 tacatcttcc attttatctt cctgacctac atttccacaa acagctataa catctatatc
     1201 ttcataattt tcctttaacc aaggtattat gattgatgtg tcaagtcctc ctgaatatgc
     1261 taatactact ttttccttca tttttaattc ctcccaatct taaattataa tttttatttt
     1321 atagaaattc ttaatattga atgactctga gaatttagac tcacataaaa tcattttaat
     1381 actaaaaaaa ccccttagta ttttaaaata ctaagaggcg aaatatccgc ggtaccactc
     1441 ttattgataa acttagctaa tcaattagta aatgttcaac atactagata aacactaata
     1501 attaaatata ttaaaaaacg cctcttggaa aaaccaagag acgaaatata tccgcgttac
     1561 cactctaatt gataagataa acttatccac tctttctctt aaggcgagat tacctactaa
     1621 catactaaat tcaattagta tcctccaaag ttcttttcac taatacttat ttgccaagct
     1681 tccaccatcc tcagctctct ataaaacaca taaaagttac tctctttctc acaggattta
     1741 aatataaagt tttatatctc tcttataaaa tatataatag tttatttttc catttatgtc
     1801 aactatataa tttattttat ttttatttta aagagagttt tatataattg actaatatgt
     1861 tactttccaa agacataatc ctttagctgg tgcagtatct gatgattgac atctatcttt
     1921 tgattcaagc atatacttaa tatcttgtgg agctcttctt tttagaccaa catctatcaa
     1981 agcacctact attatcctta ccatgttata caaaaatcca tttccttcta catatatttc
     2041 tataacatta tcatcttctt ttatatttat agaataaatt tctcttacat ttgatttttt
     2101 cttagattta gaagaagcaa aactagtaaa atcatgttct cctactaaat actcacttgc
     2161 ttttctcatt ctatctaaat ctaatttttt acttacatga gtagcatatt ttcttataaa
     2221 aggattatga tacttattat tgtcaatttt atacatataa gtctttgatt ttacattgta
     2281 tcttgaatga aatctttcat caacttcttc aacagttttt acaactatat cttctggtaa
     2341 atattcatat aaatattttt gaattttatc caaagaatcc atgcaattag ttctaaaatt
     2401 taccacttgt cctcttgcat gagctcccat atcagttctt ccagaaccta ttatctccac
     2461 tgtttcatta gtcattttac ttaaaacatt ttcaagtttt tcttgaatag tcatattatt
     2521 gtcacctaat ttttgatatc ctttatatct tgaaccatcg tatgctacta tcatttttat
     2581 gtttctcatc ttaatcctct ttctaagaat ttatgcttgt ttagcaaatt tattttttat
     2641 attaaaagtt atttttgttc caacattctt ttcactttct attttcattc ttccaccatg
     2701 aagttttaca atttcattac taattgctaa tcctaatcca ctaccagcct tattataatc
     2761 ttcttggaaa aatttatcta acactttatt taaattttgc ttttctatac ctgaaccatt
     2821 atcctctact gatatggttg ttatctcttc atcttgagaa gctacaactt ttatataacc
     2881 accttgactc gtaaatttaa atgaattttg tattaaattt atgagaacct gtcttaatct
     2941 atttttatct ccttgaatgt tctctataaa ttcattttca aattcaaata aaagtgatat
     3001 atctttttca gctgctttaa ctttaagttg atttacaaca cctacaatta aaccttcaac
     3061 atcaactata tcaacatgaa gttttatcct gtctgaagaa agccttgaaa agtctaataa
     3121 ttcttcaact agttttataa gtctttctgt ttcatcttgt ataatgccta aacctaagtc
     3181 tagctcttct cttgttatac tttcataacc taaggtttca ctccacccct ttatagatgt
     3241 aagaggagtc cttagttcat gagatacaga tgatataaat tcctctttta atttttcact
     3301 tttgtcaatc tcatgtgcca tatgttcaaa tgtctgtgct aaatcaccaa tttcatcatc
     3361 tacaatcttc atcttttcta attttatatt ataatttcct acagccaatt catttgcaaa
     3421 tttctttaac tctcgtaatg gttttataag agtctcagca aatttaagac ttattagtat
     3481 agctataata agtataaata ttcctgctaa tataagccat gtagcttgct ttaatattgc
     3541 attatcaata gcatctaatg atactgtata tctaacaaca ccttctatta tattatttac
     3601 cttaagtggt aacgagatac tcataacatg ctcccctgta tctggtattc tataggtata
     3661 gggtgttaaa tttttagttt ttatatcttt taatgcattg ttaacatctt catagtttgc
     3721 tttttcttta cttttaaaac catattggtc taatataata tttttatctt tatctattat
     3781 tgaaattcca aattctgaat ttttagttaa gggttgttta tcaaatatat tttttacttt
     3841 tttctcaaaa cttgtagttt ccattgtaat agcattatat tcatcagcat attttatttc
     3901 agattcaagt agttgtttga cagaatcata atagtagttt tgtatgtaaa acatgaacaa
     3961 tccttcaaac agagtaacca ttataataat tattataaaa tagtttttta ttactttttt
     4021 tctcagacct tcaaaatgta acactattat tcttcctttc taaaacagta tccataaccc
     4081 caaatagttt gtatatattt tggttttgat gggtcatctt ctattttatt tcttatcctt
     4141 cttatattta catctactat tttaaaatca tataaatagt tagttcccca tacttcgtct
     4201 aatatctggt ctctacttaa cgactgttta gcatttgata ttagatattt cactattgaa
     4261 aattcagttg gtgtaagctc aatttccttt ccatttttaa ataattttct tttgtctata
     4321 tcaagtataa atggtggaga aactatctca ctcgacttaa cacttgaatc atcctttgct
     4381 actcttctaa gaagagcaga aactctgact aaaagctctt ttatactaaa tggttttatg
     4441 atgtaatcat ctgctcctga aataaatcct tctattttat catcttcttg agctttagca
     4501 gtaagcatta ttatacctac ttggtcaaaa ttctccctta taaatttaca tacttctata
     4561 ccacttatat ctggaagcat cacatctaaa agagctattg taatatcctt ttctgttgtc
     4621 attttttcta tagcttctct tccagtacct gcctcaacta tttcatagcc ttctcttttt
     4681 aagtttatac taacaaaact tctaatacca atttcatctt ctagtataag tatcttttcc
     4741 ttcataaaat aaaacctcca attgagtata atattaacca tatattaatg agaaatattc
     4801 ttttaatgcg tctatagtca tatctagctt atccatttca tttgcattgt ttacaactag
     4861 taagaaagta tattcatcgc tctcttgaag tacaatagaa ttttgagtac ctgtattttt
     4921 tctatcttca agtttatttt tgctctctac tactatatta aataaattct tacggtctga
     4981 atagtcaaaa tagtaaaatt taaataccgc tttttctcca acaaattcct gttcaacaag
     5041 tattttatca aataaattat ttggaataaa taatttaaaa ttatatttat aattataata
     5101 tatctgactt gtaaatatta aacttgagtc agcaccttct tttccattcc atctatacca
     5161 acttatattt gcagatgatt tagaactata agtatttcta ttattacctg tactgcctgc
     5221 aactataggt atttctataa tcttatcttt atcaatatca tctacaggta tataataaga
     5281 tttagtaata gttttatcat aatcactaaa agctttgtta attttcccat ctttcatata
     5341 aagtatctgc gtaatatagt tattatcttt caatatagga atatcaagta caataccttt
     5401 tatggctgca gatgaatttc caacagatga agcaacattg cctaatgtta tatataactc
     5461 acttaggttt tttacatttt caaacttaac agaatctttg atttccattt tcccatcaaa
     5521 atttgcaaca cttgcaaaca tttcattagt cttattattc aaatgaagca tcaatatgtc
     5581 taacttgtca tctccatcta tatatccaat ttttactttc atgtcattaa aatcttcttt
     5641 attttgaagc catgatgcat ccaagtcata tatttttgtt atttcattat ctactacttt
     5701 atatatatac atattcgttt tgtctttgga tttaaccaat aagataactt ccttatatcc
     5761 atctgagtcc aaatcataaa aattagcata ttctattgat tctccatttt ctaaaaaatg
     5821 gtctccaagt acgtattttt ctccattgta actcaaggtt acaaacccta cctgactcac
     5881 attattactt aaatcttctt tttgttcaaa tactactaat tcatcaattc catcgctgtt
     5941 taaatctacc ttatttattt ttcctacttc ttttgtatta gaaggcaata tcatagatga
     6001 atctggtttt agtaactggt caataccatc atacaatact ttttttgtat catcatagtc
     6061 aggtatcttc gctaattgtt ctggagattc agtttttata ttacatccag taacaaataa
     6121 catcatcagg gctactacag taataaactt taacactttc atttaactcc tcctaatcct
     6181 ataaatcttt aaatttgttc acaaaaatct cctcacaata attagacgtt tcaaggcgtc
     6241 ataatgtttt acttatttta ataaaatttt atattttaca aatgaatcat gtaaaactta
     6301 tttattattt ctaggaggct taggacccgg atattggaaa aagttagttt ttaacatacc
     6361 attatacaat tttctttttt tgtctgcttt ttgcccaaac tcatattcga aatcttcata
     6421 agatgttatc aaataatatg accaattttt taattttcta aatgcataac caagctcctt
     6481 atacaactgt ttaacactgt ctttatcttc taatctttcc ccataaggag gattagtgat
     6541 tataaaccca aactcatctt cacttttaaa ttgagttgca tctcctacat taaactctat
     6601 atattcatct acacctgcta tttcagcatt ctctctagct atatctatag attcttcatc
     6661 tatatcatat ccataaatct taaatttaga ttcattgtct atcttattaa aagcatcttt
     6721 tcttacatcc caccaaattt ttttatcaag agttctccac ttttctgata taaactctct
     6781 atttaagcca ggtgccatat ttataccaat catagcagct tcaatcaata tagtaccaga
     6841 accacacata gggtccacta aaactctacc tgctttccaa ggagttaaat aaataagccc
     6901 agcagctaaa gtctctctta ttggagcttt atttgccttt tctctatatc ctcttttatg
     6961 caaagcatct cctgttgtat ctatagaaat agttacttta tctttgtgta taaatacaaa
     7021 aatagggtat ttctccttat cttctttaag caaaccatcc tccaaataac ttttctttaa
     7081 gctttctact atagcctttt ttactatggc ttgtacatct ggtgtactgt ataattttga
     7141 ttttatagag gaagcttttg atataggaaa ttgagctcca taaggtatat atctagacca
     7201 gtttattctt tttgtatttt caaataattc atcaaagctt tttgcctcaa attcagcaat
     7261 tttaagatgt actctttcag cacatctcaa ccacatgttt gactttgcta ttccaaattc
     7321 atcagtttta tatgttatcc ttccatcttc tgtttttatt atttcatatc ctaaattagt
     7381 aatctctcta gccagcattt tttccattcc aaaaaaacat ggcgatatta atgtataatt
     7441 tttcataaaa cctcctgttt ccttatatta actataaaac ttatttattt ttaaaataat
     7501 ctacacattt aaattcatac ttcccatttt atatcctgac atatctagtg taacatattt
     7561 aaagccaaat atttttaatt tagtatcaac tttattaaaa ttgttatttt caaaaaattt
     7621 acctagttct tcctgtccaa cttctattct agctatatct ccatgcattc tcactctaaa
     7681 ttgagaaaac cctaaattga atagatattc ttcactcttt tctactattc ttaaattttc
     7741 atctgttatc tcaactccat aaggtatcct acttgcaaga catgcaaatg atggtttatt
     7801 aaaagtattt aatcctaatg tttttgaaag cgacctaatt tcttcttttt ttaaactact
     7861 atcttgtaat ggactaatca ctcctaattc actcaatgcc ttaagcccag gtctatagtc
     7921 tcctaaatca tccatatttg tcccatctac tatgtacttt atattgtgtt cttttgctac
     7981 ctcttttatt ttagagaata tatatttttt acaatgatag catctatcaa caccattttc
     8041 tttaaattct tttaaatcaa agttttctat atttaatatg atatgtttaa caccaaaatt
     8101 ttgagtatat tgtttagcct cttcaatttc cctacttgaa tgcatcattg cgtgaatagt
     8161 cacagctata acattttcac ctaatgtatc ttttgcgacc ttaagcaaaa aattactatc
     8221 tactccacca gaataagcaa ctacaacact gcctaattca agtaatcttt tttttaattt
     8281 atctaatttt tctttttcct tcacaaaact tacctccata tctggaaaaa tatgaactat
     8341 aaaactatat attatttttt acattcttaa tgattataac atataaagta tttattttat
     8401 aaattataat tacctttaaa tctattaatt ataatattaa aaatattaaa gtattttttt
     8461 aacctttata atttaaacat attgctgtgc tatactgtgt ataaaaacct agtttgacac
     8521 actccccata actaaagcaa ggggattctt ggtgactaac aatttaatac tatctaaatg
     8581 atagaatatc aatcccacca aaaaagcact attgtagagc gttttaatac atccttaaga
     8641 gcctttaagg tttcataccc ttgtataata atatcctcac aataaaatac taattcaata
     8701 cataagtcat caaatttttt accatcttta gttaaatttt ttacagaata ttgaactgta
     8761 tcacatccaa ttgcgattgg aaagattcca tgcttttcat accaatattt tgcaatagct
     8821 atatgttctt catctctagg acaatcatta aatccaccaa taggtatgta tgcaaatact
     8881 tcatatggtt taattgttgg aattttagct atgtaaactt tgttatattc atgaaatata
     8941 tctacattca taattggtct aaaatgtcta taatcataaa aatcatcttc taaatcaatt
     9001 agctccttta ataattctga aatctcactc ttcctaaaat caaaaaattg atttacatca
     9061 atttcattat attttgttaa acaatactct ctatagtttt caagagaacc atactcttta
     9121 gtaatcatat ctatattttt tcttaatagc cctacttttt catcaatgac tataattggt
     9181 gtatattctt tttcttttcc atcttctatc atcttttcaa ataaaacatc aatatcaatc
     9241 tcattgttca aaggaacaca ttcaaaatca tataaatctt caatacccat atttatctcc
     9301 tattcttttt agatttaatt ttttgaaagt tttattttta ttgtcagatt tttaatacca
     9361 ctattattta aactataaat atagaggcaa tttacaaaat atacttatat aaagcaaaaa
     9421 ttcatttcgt ttttctatat aaaactgtat gaacaagata ttttttatga caatcccctt
     9481 atataaatat gatagcacaa aacaaatgtt catggttaat aataagtaat tttgtaataa
     9541 ttcgttgtaa tattataata ttttaatata aaacatatga tataatttgt aataag
//
LOCUS       DAEMAE010000141         9348 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEMAE010000141 DAEMAE010000000
VERSION     DAEMAE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.143"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..322)
                     /locus_tag="KPH93_002623"
     CDS             complement(<1..322)
                     /locus_tag="KPH93_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689430.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKVGFPKF
                     KSKKI"
     gene            complement(967..1269)
                     /locus_tag="KPH93_002624"
                     /pseudo
     CDS             complement(967..1269)
                     /locus_tag="KPH93_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(1466..2788)
                     /locus_tag="KPH93_002625"
     CDS             complement(1466..2788)
                     /locus_tag="KPH93_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689431.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(3145..4785)
                     /locus_tag="KPH93_002626"
     CDS             complement(3145..4785)
                     /locus_tag="KPH93_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG1689432.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAEGKQTDVCAGRVIKNPLSIAK"
     gene            complement(5095..5751)
                     /locus_tag="KPH93_002627"
     CDS             complement(5095..5751)
                     /locus_tag="KPH93_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689433.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(5777..6478)
                     /locus_tag="KPH93_002628"
     CDS             complement(5777..6478)
                     /locus_tag="KPH93_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG1689434.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKAALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLTGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            7283..7762
                     /locus_tag="KPH93_002629"
     CDS             7283..7762
                     /locus_tag="KPH93_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1689435.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDFLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDIFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            7759..8538
                     /locus_tag="KPH93_002630"
     CDS             7759..8538
                     /locus_tag="KPH93_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689436.1"
                     /translation="MNKNILNNSFPEIPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFGKGLVATLAITLILGTTALAVGQISSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IISEFSNGYKFKGAYTVNEKALDKEDNVLGELKALSFEYKNGNDEISLKTANNVLGNS
                     SENLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKVDEKSGKYTFSVGPNNESGKVE
                     INEFKFLTWKQDGVYYSFTVQDSNLSMNELVKMVHEVIDAK"
     gene            complement(8746..9246)
                     /locus_tag="KPH93_002631"
     CDS             complement(8746..9246)
                     /locus_tag="KPH93_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689437.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVLKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         3170 a         1681 c         1073 g         3424 t
ORIGIN      
        1 taattttctt tgatttaaat ttaggaaatc ctactttttc tttaaagaat tttttatatg
       61 cattatctaa atcttttaag gcgttttgta gtgaaaattt gtcaggttct ttaagccact
      121 ttaattcttt ttttaagttg gtcaaatcag aactacactg tttataagta aaagtttctt
      181 tatcgttttt ataaacttct attcttttag caagatactt attatataca aatctacaac
      241 atccaaaagt tttgttaatt aactcttgtt gttttttatt tggatacatt ctaaacttat
      301 aagctttttc cactactatc acctcacttt aactgttttt ctgattatta atatacgatt
      361 attagtatac atgttctcta ccatgagtaa cttccttttt tcatcactca ttataatatt
      421 aacaaacgta attatttaaa acaattattt cgaaggttta tatatttatt tttaaataac
      481 atatggaaga accgtaagta ctaactttta tcatttttga ggttgtcgtt cacataagtt
      541 cgctactacc tatgcagttc tcttatgaac ttcttacact ttcatgcaag cacagactat
      601 atcttatcct tcggcattat ccgttaaggt ctacccactt ccactaccga taacttgtag
      661 tgtacttccc tcaagaggaa tagtcgttga agtttctcct attcggagtt tacctgctga
      721 ttgcccattt taaagtactt agggtttaac cttataccat ctaatcaatt ttttctactt
      781 tcgtaacttt cacacttagg tttatttcat ccttatgttt tagtttgatt agctttaggg
      841 gtttccagca attcaagtag tattggatag gttctatcct atctctacat ataggtttcc
      901 ctatatgctg actattttag ctactaactc atgactaaaa tcacgagtgt gcgtagctat
      961 tttttaatca aaaagtccca taaaatgccc catactcatt acccattcac tattatcaat
     1021 ctcatttgtt cctgcaatga aaatatcttg tcgtctttgc atattactca atgatttttt
     1081 taaaggcctc caaaatacat ctgctacatt ttcagtccct tcttgttcac gaaatggata
     1141 cacacctttc cagttataat ctgatttcat atgttgcttt aagacatttt caacctcatc
     1201 tccagtagca ttttctaatg cttcaaaata atttcttaca actttttttg aatcctgata
     1261 ttttcccata actatctcct cccagattta tttaagttat aaattaactt tcttatttta
     1321 tgcatacgtt ttccctttta atgatttttt agctgtttta agtttatttt atgaatacat
     1381 atgcattttt ctaaatatta acatatatct aaaaaattgt aaagaaaatg ttatcataaa
     1441 aatttaattt ttgacttata gctatttaaa acaacaaaaa aggtttaaaa cgcagtattc
     1501 tactgggtct accatacttg ctcataagct gttcgctcac tacctcacaa taaccagaat
     1561 cacacaatct aagtataatc ctgtccatag ttctaatact tacaccacat atagtagcta
     1621 attcctttga tgtaaattca gtttttccac tatttattat tttattaaat actttatata
     1681 cagtattagc acttagccct gtctctgtag caatttgata aaaacgttca tcaaaactat
     1741 ttttctcttt gacagtagga tttggcttta gtggtcccat aatttctcca ttttcaaaaa
     1801 ctacatatgc tgcatcattt ttttgacttt gagccatctt aatactctca taagcgttaa
     1861 atttagcttc atttgctgtt tttccataac caattcctat ataagtgttt ataactgaaa
     1921 cttcacgaat catatccaac aaataaatat ctctgtattt ctttgtttct aactcaagta
     1981 tttttttagt tgtaaaaatc ataaattcac ttctactctg ctctaccaaa acaccatcta
     2041 tacggctatt gaaatggtat agtttatcaa gaatcttaat cctttgtgaa agataagcgt
     2101 actcatcttc cttcatcagt gaatattctg aaggcatatt cagtttaata gcaagaacaa
     2161 caatctgatt ctcattgttt ttctttgcaa tataacgcat ctgaatgttt tttatacttt
     2221 gcaccattac gtcctcagaa ggcaaggcta aaactactgg aatattatct tttttcatta
     2281 tatgataggc ctcttcaaga cctgtaatac aacaacatat atcattattt tgataattat
     2341 atttatgaaa ttcaagcaca aactttatat agtcctcttc taaatacttt tgctctgcaa
     2401 aaaacatatg ttcatcctta aattcaactc caatttcttt atacaatctc ttcatcatag
     2461 ccttatcata agtatctata ctaagtctac taatatcaaa tttaagcaaa tatgtaattt
     2521 caagcaacac tctgtacaat gttgtttcat gtctaggaat ataatcagac aatacaaaat
     2581 ctttttgtct tttttcaaaa aatttaaatg gcgacttacc tgtaaaaaga actgcatcca
     2641 ctctacctct atatttttcc aataaatcag gtacttcaat atatctatca tatctaagaa
     2701 aatctgcttc aatattagga aaattctttt tacttactgc tagtatttta tcgattacca
     2761 tacctggtcc tacataaagt attttcattt cgtctctcct ttcctaatga tactattatc
     2821 tcttaaaaca ttttctaaac ctaccatgac taaagtcaca ggactcctga ttcatagaat
     2881 tttgaataat caattatacc taatataaaa agttacttta agttttttaa ttacgactat
     2941 ctgttgaaac atttttgtat ttaaattttt gtatttaaat cttattcata aatattaatt
     3001 acattttaca attaatatat tgttttttct acttaaagtt aaattcttta gaaaacacaa
     3061 ttataacaat ctttattaat ctaacaaaaa tagccttcct tatatttaaa gaaagactat
     3121 ttttataaaa gttcttaatg tattctattt tgctatgcta agaggatttt taattacacg
     3181 tccagcacat acatcagttt gctttccttc tgccaacgca attttaccat tcaccaacac
     3241 atattccact cccactgtat actgcttagg attactataa gtcgctttat cttctattgt
     3301 atctgggtca aacaccacaa tgtcagcaaa ataattttcc ttaagtagac ctctttcttt
     3361 ataaatgcct aagcgttttg ctgaaagata agttatcttt ttaactgctt cctcaaaagt
     3421 caacatacct ttttctctaa catagtaaga gaaaattcgt ccaaaagtac caaatgcacg
     3481 aggatgaggc tttccaagag aaagtaaacc ttctgtagaa aggtccatac tatcagaacc
     3541 tatacatgac tttggatggc aaacgacctt atctgtatcc tcttgacctt gtgtatgaac
     3601 aactacttga attttacttt tttcttcaat caaaagccta aagaatgtat caaatggatt
     3661 ttcaccattg attttggcta cctccattaa agttttaccc tctacatatt tatttgcttc
     3721 agtcattaca gaggctagta caacaccgtt ccacccacct gctaattggt agaaacattg
     3781 ccatccagga atcccttcct ccaactcttt tataattctg tctctatttt cttgtacttc
     3841 taatcgtttt accatgtttt ctacgccgcc ttcaaaagcc caaggaggta aaagtgcacc
     3901 caaagatgtc attccagctg tatatgggta acaatcaaaa gttacttcca aaccatctgc
     3961 ctcagcttca tctagctttt taaatacctc atcaatagtt ccaaaattct tatgacccat
     4021 gacttttaaa tgagaaatat gtagaggaac tcctgcagtc ttagctatat aaattgcttc
     4081 atcaagagct tccatttctt catcaccttc atctctaata tgagatatat agtatccatt
     4141 gtatggtatc aattccttac ataattcagc catttcttct ttctttgtgt atgctcctgg
     4201 taaataaact aatcctgatg aaagagctac acatccagct tccatagctt ttctaacttc
     4261 caacttcata tttttaagtt gctctaaagt agctggttcc tttgaaaatc ccatttcatt
     4321 gatacgtaca ggtccttgtg ctacataagc tacaacattg actgctggag aattttcagt
     4381 taagtaatca aagtattctt gcattgtatt ccaatgaagc tctaattttg ttggaattgt
     4441 accaattatc ctagttccaa gatagtcaat taactcttgc ttcttctctt ctcttatcgg
     4501 agcaactcct ataccacagt ttgcaataac atctgttgtt acacctgtat ggatacggct
     4561 tcttgtgtat ttatcataca caagagataa atcagaatga gtatggaaat ctataaaacc
     4621 aggtgataca aattttccag ttgcgtctat agtctcttta gcctcttttt gacttaaatc
     4681 tccaatagcc atgattttgt ctcctacaat ccctacatca cttttatatc ttgaatttcc
     4741 tgtaccatca acgattattc cattgacaat tttaacatca aacatgctta ttctcctttc
     4801 aagacttatt tatattcata tttcttaata aacaatccta ttcttttttc tcaacaaata
     4861 attctagtct ttctccttaa taaacaatcc tgttcctttt cttaacaaat aattctagtc
     4921 tttctcctta ataaacaatc ctgttccttt tctcaacaaa ttattctagt ctttctcctt
     4981 aataaacaat cctattcttt ttcttaacaa ataattctag tctttctcct taataaacaa
     5041 tcctattctt ttttctcaac aaataattct agtcttcctc cttaataagc aatcctattc
     5101 ttttgtttta ttgttatcaa ttacaaagaa gacccttgtg ataagtatcc cacctaacac
     5161 aataaacaat gttaataatc cacctggtat accaattttt tcaccaaagt acataattgc
     5221 taacgctaca gcaatcgtac atccaccagc tcgaatatcc ttacgtgcaa tatcaacata
     5281 aattgctcca aataatgctg gaagtatata attaaatgaa ttagtaacaa attcaggtaa
     5341 caaaggaatt actttatagc ctacaatggt aaaaatggta atcatagaag ctgatacaaa
     5401 gattgagcaa catgtaccaa ttgttccaac aacttcaccc tcatgagttc ctgcttctac
     5461 acctgcaatt ttctgtgcca ttgatgctgc aggcatacgt atatctccaa cagaacctgc
     5521 aagccaacct atataagaac ctggagctcc tagtgtcgga taataagcta taggttgaac
     5581 aatccaacta accccataag tcgcagcgac taacccccaa atttgaaata taacagaaag
     5641 cattggcatc tgaccaaagc gaatacctac atagatagcg ggaataaagt ttgcaataat
     5701 tgcaagacct atagaaattc tccctatttt tatcatcttt ttatcataca taattatcac
     5761 ctcttatccg ttttaatcac gataatataa tctgtgtacc aaacatacca attaacatgg
     5821 caatacctaa ggataattct tgtaatcgtt gattcttttt aaataaagtt gtcaatacta
     5881 gcataactat agctgaaata attgctggtg taagttttgg tatttcaaat gtttttattt
     5941 gatttgaaag aaggtaagca aataatccaa ctgtagctcc tgtaagtagt tttttaaccc
     6001 aagtaggatt atacttcatg ttcatctttt caacaattcc acgcattcta tgacccaaaa
     6061 taaatacaac aaacagccaa cctaagttat ttaaagccat tccccatagt gcatatgaaa
     6121 aagcttctgc tccaaatgca gcactaccta cttctacccc agataaattg gatgtaagag
     6181 atattactcc aagctctgtt ctagcagctc cgacatcaca gagcctcatc caagtcgtag
     6241 gagctccaat cacagctacc agtgataata atgtaataac aggagataga gaaggtccaa
     6301 ttgctgtaac actagctgag cgaattgctg ctttaattcc tttcctttca ataccaagat
     6361 ttgaagcctc ttttagcgct gctttcataa atacgattgc ttgaacaact gaaataatta
     6421 ctaaaaaact agaaacaatc caaagacctg ggctattaat catttcttga aacttcatac
     6481 tttattcctc cttaaaataa attttttaat gttttattgt gagctttctg taatcttgtt
     6541 atgtttttat attgttttaa tttattttat ctaagatata gtatctttaa taggagatta
     6601 tctgtcttaa agtctcacta aaaacattaa tacttaaata ttttattgat aattggtaaa
     6661 tatcagaaaa ttcacatatt atttttttaa cctgtaatta acttataagg tcttttatta
     6721 ttttgacatt tcaatataaa acttttaatc aaaaaacttt attattttaa aaaaaattat
     6781 tatttttata atttatattt tttaagttta tttatgttca tctctatcta aaataatgac
     6841 atttctaact cttaattctc atttaagttt cattgttttt tcttaaaagc ctacttttag
     6901 tcaatatact gttatcttta ctcattaatt aaagaactta ttttaaatct atacatgaag
     6961 taaataatct aaaagcatta ttttctaact actcaacatt aaatacttac tattttctaa
     7021 ttactcaata taaacactta ctataaaact aaatatataa atagcttcta aaatttaata
     7081 tatctacatg gctttatata tgacgggaaa aaatactaga aaaaaatata ttatagaata
     7141 tttgtataga ataggcaaaa ctttcaaact taaatttatt aaataatcat aaaaaaagtg
     7201 aaccatctat agtaaaattt tatctaataa gtgaaaggca aaaaagtagc gctacgattt
     7261 gcatagaaag gaaggaactc agttggataa aactacattt acaaacaata tacttgaatc
     7321 agaacagact ttgtatcgag tatctaagtc tattctagga aacgaccaag actgtgaaga
     7381 tgccgtcaac aatgccatct taaaagcata tgaaaagttg gattttctta aggaagaaca
     7441 atactttaaa acttggttga ttagaatagt cataaatgaa tgtaattcat taagacgaaa
     7501 acgattaaaa tcattatcat ttgaagacat cttcaaaaac aaaaaaattg atgaaaaaga
     7561 tgactacagc gatttgtata ctgctattca aagtctacct aaaaaaatta aaattccaat
     7621 agtcctttac tatattgaag gttattcagt agatgaagtt aaggaaatat tagatatacc
     7681 tcaaggaact gtaaaaagta gattatcaag aggaagaaga ttattaaaaa ctaaattaga
     7741 aaacacggag gtaatcatat gaataaaaat atattaaata attcgtttcc agaaattcca
     7801 gaaagttttc ataatagttt aagtaaaaca ctaaatagtt taccagaaag ggaagaaaat
     7861 tataaaatga ttaataataa aacacataaa ttaccatttg gaaaaggctt agttgcaact
     7921 ttggccatta ctttaatatt gggcacaaca gcccttgcag taggacagat atccagtcta
     7981 gttggaaaat caagtacgaa agctacctat acatctatcc caactacaga gcaagtaaaa
     8041 aaagacttta actttacccc taatataatt tctgaattta gtaatggata taaatttaaa
     8101 ggtgcatata ctgtaaatga aaaagcactt gacaaagaag ataatgtttt aggagaatta
     8161 aaagcactta gttttgagta taaaaatggt aatgatgaaa tatcacttaa aacagcaaat
     8221 aatgtacttg gaaatagttc tgaaaatcta aaagttgtag atacttacaa agatattaat
     8281 atttcttatg aagcatttgc acaaaagttt gtaccagaag gttataaaat gacagaacag
     8341 gataaagttg atgaaaaatc aggcaaatac acatttagtg taggtccaaa taatgaatca
     8401 ggcaaagttg aaattaatga atttaagttt ttaacatgga aacaagatgg agtttactat
     8461 tcttttacag ttcaagactc aaatttatct atgaatgaat tagttaaaat ggttcatgaa
     8521 gtaatagatg caaaataaac ataagtaata ataagttgtt tatgagttta ttatttacga
     8581 ctttaatttt tttattagta aacatggttg tatcttccat ctttatattt tctttaaatt
     8641 taatatataa aattttagat atttaactac tttcaaaaga accactatta aaaagccctt
     8701 aaaaatattt catttatatg gaattttaag ggctttaact ttattttata taagtgtaag
     8761 ctccatttga attacctctt cttttacagg tctatcattc catgtaaaac cactagatat
     8821 tgtatttacc tctacaaacc ctaaggattt ataaacatga atagctctac tattactttt
     8881 caacacatca agctcaattt tttcataccc atattctttt gataaatcaa taatttgttt
     8941 taaaagacta cttccgatac ctttttccct atactcttta agaataccca tgcctaaata
     9001 accaactttt tcagttttag gcaaaacatc acaccaacca atacagttat ctgattctaa
     9061 atcaattata aacagttgtg gtaagttttt atttatagta ttttttataa attcaactgt
     9121 ttcttcaaaa ggaaaggctt catccatagc tagatatttc ctctcttttg ctattttatc
     9181 aaaagtttgc caatacgaat aaatatactt ttcttctgcc tttataaatt ttatgccttc
     9241 tatcaactta acatttcccc tttttaattt atgattgtag ttaacttatt ttatatgatt
     9301 ctagtttata atgctatata aattactata tattagctta aaatatct
//
LOCUS       DAEMAE010000142         9335 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEMAE010000142 DAEMAE010000000
VERSION     DAEMAE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.144"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..742
                     /locus_tag="KPH93_002632"
     CDS             113..742
                     /locus_tag="KPH93_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689438.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGKAVFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGSEDKYDIVEMFYCKDGCNNGDGL"
     gene            743..1525
                     /locus_tag="KPH93_002633"
     CDS             743..1525
                     /locus_tag="KPH93_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689439.1"
                     /translation="MVNKFNNKFLRLVKTVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTSLKMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1518..2396
                     /locus_tag="KPH93_002634"
     CDS             1518..2396
                     /locus_tag="KPH93_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689440.1"
                     /translation="MNNLVCDSGYIIRNLYKSYEVDNEEHVVLSDISLNIDRKHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKMINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLFMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKGI"
     gene            2429..3388
                     /locus_tag="KPH93_002635"
     CDS             2429..3388
                     /locus_tag="KPH93_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1689441.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3702..4394
                     /locus_tag="KPH93_002636"
     CDS             3702..4394
                     /locus_tag="KPH93_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689442.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4404..5408
                     /locus_tag="KPH93_002637"
     CDS             4404..5408
                     /locus_tag="KPH93_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689443.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESCLYLGSSFLGRNISVILKQLHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIREEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQIDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSISFNKDVNSLIKV
                     "
     gene            5663..6514
                     /locus_tag="KPH93_002638"
     CDS             5663..6514
                     /locus_tag="KPH93_002638"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG1689444.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAIYRIIMGVVLAVLA
                     FTNIISV"
     gene            6616..7362
                     /locus_tag="KPH93_002639"
     CDS             6616..7362
                     /locus_tag="KPH93_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689445.1"
                     /translation="MKILTVNNLSKVHGKKIIFNALNDINFSIEYGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNLLLLKGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDKRLNEVSEILGIKDLLGKRTFEVSGGQAQRTAIARALINK
                     PSILLADEPTGNLDSKSSKTVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     AIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7362..9278
                     /locus_tag="KPH93_002640"
     CDS             7362..9278
                     /locus_tag="KPH93_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689446.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIIISSSLLFSFIMFIAHPDL
                     DVSQFPDYLKEGYNMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIIKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAVTST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFIKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVNRQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMRFIENNLKKENIDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITEVIG
                     VKPISFEDNESISLLSSSLPGNKKIKENVIVGNRSLKVVKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIET
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYIVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3644 a          886 c         1646 g         3159 t
ORIGIN      
        1 atgtagataa aaattgtttt attaagaaaa aattaatata ttgacaaaat ttaagtgctc
       61 atttaaacta ttaattgata ataatattca atattaaata ataggaggcg ttatgaataa
      121 atatcttcta ataaatccag tggcagaaag aatgtatgga gaaaagtttc accttattaa
      181 agcaaatttg ataaaaaagg ggtacataat agcagaatgt gaaccacagt tagaatatgt
      241 aaaaaagcag tacaaagaat atacaaaaaa gactgaaaat acaatgttgg attgtagatg
      301 tccagaaagt ataagccttt taaaaagaaa taatttgata aataattttg aagtacctat
      361 gatagaacca atattagtaa gaacttgtag ggttttatac gataaatata taaaaagtaa
      421 agatgatatg ttggtagtaa cctgtccatg tacacaatta agagactttg caagtgaaaa
      481 gtttaaggat aagggtaagg ctgtgtttta tacatggaaa gagtttgctg aacaagaagg
      541 tgtaaaatca cttggaaaaa tagatgaatc accaattcca ttaggatatt tcaaagatac
      601 ttttgaaaaa gttcttgaag tatcaagtga agacgaaata ttaagtgaaa tacaaaaaat
      661 aagaaatggc tctgaagata agtatgacat agtagaaatg ttttattgta aagatggttg
      721 taacaatgga gatggtttgt aaatggtcaa taaatttaat aataagtttt taaggctagt
      781 aaagactgtt ctgatatttt ttataattct tttattatgg aaaattacaa attatcttgg
      841 tatttggagt gattatatat taccatcacc cgaaaaggta tatagtacat ctttaaagat
      901 gataagtgat ggttcaatat ttattaatgt atatgcaagt atgaaaagag ttttgatagg
      961 ttttgcaata agtactgcta taggtgtgcc tttagggata ttctttggaa tttatagtgg
     1021 agtatacgaa tactttaaat ctctaataaa ttttttaagg aatacacctc cccttgcttt
     1081 aataccaatg cttatattat ggtttggtat aggagaggaa tcaaaaatta taattatagt
     1141 tttagcatcc tttttcccaa tctttacaag cacattgaaa gggattaaaa attgtgactc
     1201 aaaactgatt gaagtaggta gagtgtttga gttttcaaag cttcagataa tatttaaaat
     1261 aataatacca aatgccattc ttgatattgc tgttgggtta aagttagctc ttggatatag
     1321 ctttagagca attattgggg cagaacttgt agcagcttca tctggtcttg gatacttgat
     1381 atctgatgga aaagaaatgt ctagaacaga tgttgttatt gtaggtataa tcgtaattgg
     1441 acttttagga ataattacag attacatatt ttcgattatt gtgaagaagg taagtaaggg
     1501 aaagatggtg gaagcatatg aataatttag tatgtgactc tggatatata attagaaatt
     1561 tatataaaag ttatgaagtt gacaatgaag aacatgtagt tcttagtgat atctctttaa
     1621 acatagatag aaagcatata acagtaattt taggtgaaag tggttgtgga aagactacct
     1681 tgcttagagc aattgcatca cttgaaggta taaatagtgg tgaaattagg tttattaatg
     1741 ataataaaga gtatagacct aatgttggtt ttgtttttca ggagagtaga ttgatgcctt
     1801 ggttaaacgt aagtgaaaat atagcttttc ataatcaaag aaaaaatact attttaaaca
     1861 aggtgcttag aaaatttaaa cacaataaaa gaattattgg taaaatgata aatgataatt
     1921 ttagaagctt agaaacaaat gataatttga acaaatcaaa taaatataat aggaaaagta
     1981 atattgatgt agaaaaatat cttaagatga tgaacttgca gaaatttaaa aactcttatc
     2041 caaatgaatt gtcaggaggt atggcacaga gagtaagcat agctagagct ctttctttca
     2101 atccagatat gttgtttatg gacgagcctt tttcggcatt agattatttt acaaggattg
     2161 atatgcaaaa tgaagttata agaatacata agtcaactaa taaaggcgta atatttgtaa
     2221 ctcatgatat tgatgaagca ttaaagatag gcaaaaaaat agttgttttt acagacgaaa
     2281 gaaaaataaa agaatttaat atagaagatg gttataatag agatttaact tcagagtact
     2341 atattaaatt aaaaaaagat attttaatga caatgaaatc aaataaaggc atttaaataa
     2401 attaataata ttttaaggag tagagtaaat gaaattaaaa aagctattga taggtataac
     2461 gatattaaca cttgcaacag catctttagc tggatgtgct aaaaaaaata atggagaaaa
     2521 actttcagaa ataaatttaa cttatgtaaa atcaccatta aatgttcctt ctataattca
     2581 aaaacaggat gatttatttg gaaaggaatt taaaaaggat aatataaaag ttaactttca
     2641 cgaaattact acaggtccag agcaaacaca ggctttagct gctggagaaa tagacttctt
     2701 acatgcacta ggtggtacgt cagctttaat agctgcatct aatggagtag agttaaagat
     2761 attaaacact tatagcaggt caccaaaagg atttatgatt ttgactaata ataattcaat
     2821 aaaatctgca gcagatttag taggtaagaa ggtggcaggt ccaaaaggga ctatattaca
     2881 tcaggtattg atttcagcac ttgataaaga aggattaagt atggatgatg tggaatttgt
     2941 taatatgggt attccagaag catcagcagc tctttctgat ggtagcgtag atgctgcatt
     3001 gattgctggt ccagcagctc ttaaagctat gaagtcaggg agtaaacttg tagcaaatgg
     3061 tgaaggttta gtagatggta taatagtaac tgctgttagc acagactttg cagagaaaca
     3121 tccagaaata gttgaaagat ttatgaaggt tgaaaaggaa actttagaat atgtaaataa
     3181 taattttgat gaagctatgg aaaaagtagc taaagaagtt gatttaagtc ttgaagaaac
     3241 taaagaatta tatgcttggt atgattttag tttagatata acagataaag acatatcttc
     3301 gcttgaagat acacaagact tcttaataaa aaataagtta caagagaaaa aagtgaatat
     3361 aaaagaatta atctataatg ctaagtagaa actatgctaa aataatacta agtaagaaat
     3421 ataataaaag tttaagttac cttattaagg taactttttc tattttaaat tagtaatttc
     3481 aatttatttt agaatgacta attgataata atgattaatt aatcataaaa agatactttt
     3541 ttatagggtt ttattaagat gtctaataat atagttatac ttatgaagtc agtaattgtg
     3601 aagttagcaa tatgattaaa tttatatatt gtaaaaagat tataatacat taaaattgaa
     3661 cttttatatg atgagtaaat aggtatatta ggaggaatta tatgagttat aaagtatata
     3721 ttgtggaaga tgatatatca ataagtacat tacttaaaga ttatattgag aaatatggat
     3781 ttgaagtgat tgttgaagaa aactttgatg atataatgat tacatttgaa agttttaagc
     3841 cacacttagt tctattagat gtaaatttac ctaaatttga tggtttctac tggtgtagat
     3901 gtattaggca acagtcgaat ttacctataa tttttatatc agctagagat ggtaatgtag
     3961 accaaattag agctttagaa agtggagcag atgattatat aacaaaacct ttttactatg
     4021 atgtagtaat ggcaaaaata aagagtcata taagaagggt atatggtgaa tattcaccta
     4081 aaatagacga aagaatagtg gagctagatg gattaaagtt ttatccagaa aggttagaac
     4141 tagaattaaa tggtgagaat ctaattataa ctaaaaaaga aggtatactg attgaatgtc
     4201 ttatcaagag atacccaaag gtagtaagta gagattatct tttagaaaag atatgggatg
     4261 atattgagtt tgtagaagaa aatactttaa atgtaaatgt tagcaggata aggaaaagat
     4321 ttgaggaatt aggcatagat aacgctgttc agactgtccg agggttagga tataaattaa
     4381 ataagaattg gtaaggagaa agtatgaagt tatttttaaa agattataaa ggttatattt
     4441 ttatatatta tataggtata gttatgacaa ttatttactg caatcttatg aagtttatta
     4501 gttttggaga gagtttatat attcttttat ttagttcttt tatattgtta tgttttttaa
     4561 tatataagta ctataaaaat agagaagtat ataatctgtt tgaaaaagga gtacattctt
     4621 tggaggagtc atgtttgtat ttaggaagct cattcctggg gagaaatata tcagttattt
     4681 taaaacaact acataaattg tatcaatcaa tgatacaaga gcataaaaag atacatagtg
     4741 aacatttaac tttcatcaac caatggattc atcagatgaa aacaccaata tcagttataa
     4801 gccttcagct tcaagattat gagggagaag aagtaactga aaatataaga gaagaaatag
     4861 atagattaaa taaagggtta gatatggcta tgcattttgc aagggcagat aattttcaaa
     4921 aagactttat agttgaaaaa gccaaattaa atgaaattgt atctagtatt gtaaatgaag
     4981 aaaaaaggtt atttataaaa agtgggatga ttcctaagat tcaaatagac aagtttactt
     5041 atgtttatac agatgtaaaa tggatgaaat ttataatagg tcaattaatt acaaatggag
     5101 tgaaatattc taaagattat agtaactacc taactataaa atctgaagaa aataataagt
     5161 atgtaatttt aagtataata gatgaaggaa ttggaattgt ttctaaagat ttaaaaaggg
     5221 tatttgaccc attttttaca ggagaaaatg gtcgtaagtt tggagaatct acaggtatgg
     5281 gtctttatct agtaaaaaag gtatgtgatg atttaggtca taaagtaagt ataaagtcag
     5341 aagtaggaaa aggtacagaa gtttcaatct cctttaataa agatgtaaat agtttaataa
     5401 aggtgtaaat agtttgacaa agatgtaaat tatctgttac aatagggttt ttagttaatt
     5461 gtactttttt attaaacaaa atattcatag ttaaataatc caaatttaaa agaagttatt
     5521 tgcaaacctt acatagatgt aaggtttgtg taatgtttgc agatagttaa attttctaat
     5581 ctaaattaca attaatataa gaaataacat aagggaaata acataaaaaa gaaattttgt
     5641 aaaaaaggaa ggatgtttat tgatgagttt agatgttatt tttatattga aatcagtcat
     5701 aatagctata gtagaggggc ttacagaatt tattccagtt tcatctacag gtcatatgat
     5761 tttggttggt aatttaatag attttaaggg gcaatttgct gaaatgtttg aggttgtaat
     5821 acaacttgga gctatattag cagtggtagt actctactgg aaaaagataa aagatagcgt
     5881 tatagaattt tttaaattta tatttactgg tggaaaagaa ggaaagattg gttttagatt
     5941 tggaatgaat gtaattatag gttgcatacc atttgcgatt ataggagttc tattttacga
     6001 taatataaaa tcattgttta atttacaatc tgtaattata ggatttatag tgggtgggat
     6061 tttattatta gtagtagaaa ctttgtttag aaaaaagaat cattccacag ataatataga
     6121 taaaattact ccaatacaag ctttaaaggt tgggacatta caagttttgt ctgcatggcc
     6181 tggtatgtca agaagtgcat ctactattat gggtggatgg atagcagggc ttaattctcc
     6241 tacagcagca gaattttctt tctttttagc tgtaccagct atggtggcat cttcaggaaa
     6301 agatttattt gaatttgatt attcaataat gacaccaaca ttgtggattg ccttggtagt
     6361 tggatttata gtagctttta ttgtatcaat aatagttatg gaaaaattcg tgaatttttt
     6421 aaagaaaaaa ccaatgagag tgtttgctat atacagaata atcatgggtg tagtacttgc
     6481 agtattagct tttactaaca taattagtgt atagaaaaaa tagatttatt atctatagaa
     6541 attaaactaa gataaattat aattaacaat tatatatgtt atttcaaaaa ttagattagg
     6601 aaggatagtt aaataatgaa aattttaact gtaaataatt taagtaaagt acatggtaaa
     6661 aaaataattt tcaatgcatt aaatgacata aattttagta tagaatatgg agaatttgta
     6721 ggaattatgg ggccatctgg cagtggaaaa actactcttt taaatatgat ttctactatt
     6781 gacaagccaa ctacaggaac tatggagcta aagggcaaga atcttttatt gttaaaaggt
     6841 gaagaacttg cattatttag aagaagagaa ttaggttttg tatttcagga ttttaattta
     6901 ttggatacct taactatagg agaaaatata gtattaccat tgacattaga taaggtcagt
     6961 gtaaaagagc aagataagag gttgaatgaa gtatctgaaa ttttaggaat aaaagattta
     7021 ttaggaaaaa gaacttttga agtttcaggt ggtcaagccc agagaactgc cattgctaga
     7081 gcattaatta acaaaccatc aatactttta gcagatgaac caacaggtaa tttagattca
     7141 aagtcgtcaa agactgtaat ggagctattt caaaagataa ataaagaaaa taaagttacc
     7201 actatgatgg taactcatga cccattagca gcaagttatt gtagtagaat tttattcata
     7261 aaagatggag ctatatataa tgaaatatat aagggaagta gtagagagca gttttatcaa
     7321 gagataatgg atgtacttac attattggga ggagataatt aatgaatata aaacagtttg
     7381 ctattaacaa tatttcaaga aatattaaag catatttagg atatttagca agtattatta
     7441 tatcgtcttc actactattt tcttttatta tgtttatagc tcatccggac ttagatgtat
     7501 ctcaatttcc tgattattta aaagaaggtt ataatatgag taagataatt gcatatttgt
     7561 ttttattctt ctttgtattt tattcagtta gtgtatttct taaaagtagg tataaagaat
     7621 ttggaatact ttatattaca ggtatatcaa aacgccaagt aatgaaatta atatttatag
     7681 aaaatatgat aataaatata gtatcttctg taattggaat aataataggc ttaatctttg
     7741 caaagatatt tttagtggtt atgtctacat tcttagaatt atctccatta aaattctata
     7801 taccatttaa tgcgatattg tctactttaa tctattttat gattttagct gtattaacat
     7861 caatatttac ttcttttata attaaagaaa atcaggtgtt gaagttactt aaaggtacaa
     7921 aaactccaaa accagagcct aaaacatcac taatgcttgc tatattaagt atttgtttgt
     7981 ttataatagc gtattataat gctgtcacat caactgacca atatacaaca tacctgcgct
     8041 tagttccagt tacatctctt actatagtag ctacctactt atttttttct caatttagtg
     8101 tgttttttat aaagaagcta aaattaaaca agagatttta tagaaaaaat acaaatatgc
     8161 tttggatttc aaatttgatt tataaggtaa aggataacgc tagaatattt ttcttgataa
     8221 ctataacctc tgcagtagca tttactgcta taggtacagt ttattccttt tggaaagatg
     8281 taaatcgtca aattaattta atatatccta atacaatata ttattcaaca atgaccttgc
     8341 ataatgaccc taaaaagcct gatagcaaag aaaaagacaa ggaaagaatg agatttatag
     8401 aaaataatct aaaaaaagag aatatagact atacaagaat taatggtgaa tttaaaactg
     8461 tatttcccaa gaaaagtgat tttaatgtca gaattatgaa agaatctaag tacttagaaa
     8521 taacagaagt tataggggtt aagcccataa gttttgaaga taatgaatct atatcattat
     8581 tatcatcaag tttaccagga aataagaaaa taaaagaaaa tgttattgta gggaatagaa
     8641 gcttaaaggt ggtaaaacaa gtagaagagt gtgttatgcc agcatattat aaaaatatgt
     8701 atgtggtaaa ggacaatttt tatgatagta tagaatcaaa atttatagtg gatagattta
     8761 ctgcatttga agtagaggat tcaagtaaaa ctatagagat atgcagagaa tttgaaaata
     8821 aatttaataa tgagtcaggg atgcaaccat atttgttttt ctctaaagaa ttaatgatag
     8881 aaactggtaa attaatgtat tctacattta tatttttagc aatatttata ggtttaattt
     8941 tctttgttac tacaagtagt tttttatata ataaatttta tatggattgc caagttgata
     9001 agaaaaagta tgaacagtta aataaattgg gtatgacata taatgagatt aaaaaagctt
     9061 caacgattga gattggaata gtatttttgt ttccttatat agtggctgtt atacattcca
     9121 tatttgcact tactgcactg aaaaattctt ttgatataga agtagcatca tcagctgttt
     9181 tggtaatggg aagcttgttc ttggttcaaa tagtttattt tttaattgta agaaataatt
     9241 atttaaaaga gataaggtta aatttagtca atttttagtt actattatta aatgataaca
     9301 aaaagggttt aaaattaatt aatattactg gaatt
//
LOCUS       DAEMAE010000143         9288 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEMAE010000143 DAEMAE010000000
VERSION     DAEMAE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.145"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..1900
                     /gene="prdR"
                     /locus_tag="KPH93_002641"
     CDS             140..1900
                     /gene="prdR"
                     /locus_tag="KPH93_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG1689447.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2329..4209
                     /gene="prdA"
                     /locus_tag="KPH93_002642"
     CDS             2329..4209
                     /gene="prdA"
                     /locus_tag="KPH93_002642"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG1689448.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSAGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4290..4574
                     /locus_tag="KPH93_002643"
     CDS             4290..4574
                     /locus_tag="KPH93_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689449.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4612..5337
                     /gene="prdB"
                     /locus_tag="KPH93_002644"
     CDS             4612..5337
                     /gene="prdB"
                     /locus_tag="KPH93_002644"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5062..5064,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG1689450.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5417..6175
                     /gene="prdD"
                     /locus_tag="KPH93_002645"
     CDS             5417..6175
                     /gene="prdD"
                     /locus_tag="KPH93_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG1689451.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6190..6657
                     /locus_tag="KPH93_002646"
     CDS             6190..6657
                     /locus_tag="KPH93_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG1689452.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVGSDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6675..7157
                     /locus_tag="KPH93_002647"
     CDS             6675..7157
                     /locus_tag="KPH93_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG1689453.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7177..8184
                     /locus_tag="KPH93_002648"
     CDS             7177..8184
                     /locus_tag="KPH93_002648"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG1689454.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8304..9185
                     /locus_tag="KPH93_002649"
     CDS             8304..9185
                     /locus_tag="KPH93_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG1689455.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3635 a         1142 c         1865 g         2646 t
ORIGIN      
        1 taaagtgatt atataaattt aagtgctaat tacttaataa attttaataa taattattta
       61 ataaataatt attataatat gctagaataa atatatataa ataagttttg atgaggtact
      121 actataaagg ggggacttta tgttttcgat accagaagta aaaaaagtag aagaggtaat
      181 ggatacaaaa tttacaacta tagacgaaga cacaagaatt gaagatgcaa taaaagaaat
      241 gataaaaagc aacacaaaaa ctctaatggt aatagattca tctgaccaat taaaaggcat
      301 tatatccatg acggatatac ataatcttta tgagatgcat aaaaagtatg aaggtcaacc
      361 agtaaaactt ataatgaaga aggatgtaat atatgtcaac gaaggtttaa ccttagatga
      421 atgtagagat ataatgatat taaaaaatat agggattctt cctgttctta gagataataa
      481 aattattgga gttctaaaac aagagcatat tagggactat ttatatatgc atttagaaga
      541 ttatggattg actttgaagt atattatagg tcaaattaaa gaaggaattt gtgctataaa
      601 taacgaaggt gttgttatct tgtggaataa gtttatggag gaaaggtatg atataaagtc
      661 tgaagacata gttggtcgac cgatgaatga atttttggaa aatactattt cagaaaaagt
      721 actaaatagt aaagttggaa tgagtgactt gtattttaca gataaaaaag aaaatatgta
      781 tgcattagtt catgcaaatc ctatatttta taaagaagaa tttatcggtg ttgtatgtac
      841 agaagttgat gttacagaag ctaagatatt agctcttgaa cttgaaaaag taaatgatac
      901 attaaaatat ttaaagaacg aagtcaaaaa tttatctaaa ggtagttttg ataagatttt
      961 aggtaaaagt tataaactgg aaaaatcgaa agcgattgca aaacaagtgg ctagaacaaa
     1021 tagtagcata tttatttggg gtgaaagtgg tacaggtaaa gaggtatttg caagagctat
     1081 tcatgattat agtgagagaa aaggtcaatt tatacctgtt aactgtagtg ctataccaaa
     1141 tgagttattt gaaagtgagt tttttgggta tgaatcaggt gcatttacag gagcgagtaa
     1201 aaagggtcga ataggtatat ttgaacttgc aaaagatgga actgtatttt tagatgaaat
     1261 tgccgattta cctcttagta tgcaagcaaa gttgcttaga gtattacagg aaaaagaaat
     1321 aagaagagtt ggtggagata ctacaataaa aataaatcct agaataatat cagctaccaa
     1381 taaagatttg agtaaaatgg taaaggcaga aaaatttaga gaagatttat actatagatt
     1441 aaatgttgtt gaaataaaga ttcctccact tagagagaga aaagaagaca taggattatt
     1501 ggttcatagc tttttagagg agatatgtaa acaaaataat aagcctgttc ttacaattag
     1561 caaagatgta atagatatat ttcagaatta cagatggaaa ggtaatatta gagaattaaa
     1621 aaatacgatt gaaaatattg ttgtattgtc acaaaattct aaaatagatg tggatgatgt
     1681 accaagttat atgatggatt ctacaaataa tagtactgaa gaagaagagt atcctttaga
     1741 tttaacgaag gcaactcaaa aaattgaaat aaagaatata accaaggcac ttaaaatgtc
     1801 taatggaaat aaagctaaag cagctaagat attaaatata cccagaacaa ctttgtatta
     1861 taagatagac caatacaaaa tagatgtgtc taaaatatga cacatgacta aattttgaca
     1921 aaatgataaa ctttaattta aaacctagta aagccaacgc ttgtataagt gttgtgtata
     1981 tagatgtgtt aaaaaaaatg aaaaagtgtt gattaattga cacttttttt gtttgagaaa
     2041 atgtttacaa atacaaaaaa taacatattt taaataaata tttatataat atttggctaa
     2101 aaatagcgat agatgaagaa tctattatag aaaaaatctt tttgttaata agttggcata
     2161 ggaattgctt ataatataaa gtgactttaa gtgagaaaaa aataatatta atcataacaa
     2221 attatgtagg ttttaattta cttaatttct cagttaaggg gttacctgtt gaggaattga
     2281 gatttaaaat aataaataag aaaataatgt aaggaggtat tttatactat gtcaataact
     2341 ttagaaacag ctcaagccca tgcaaatgac ccagcagttt gttgttgtag atttgaagcg
     2401 ggaacaatta tagcgccaga aaacttagaa gatccagcaa tatttgcaga cttagaggat
     2461 tctggattat taacaatacc agaaaatgga ttaactatag gtcaagtact aggagctaag
     2521 ttaaaagaaa ctttagatgc tctttctcca atgactacag ataacgtaga aggatacaaa
     2581 gcaggagagg ctaaagaaga agtagtagaa gaaacagtag aagaagcagc tccagtatca
     2641 gaagcagcag tagttccagt aagcgcagga gttgcaggtg aaacagttaa aatacacata
     2701 ggtgaaggta agaacataag cttagagata cctttatcag tagctggtca agcaggagtt
     2761 gctgctccag tagcaaacgt tgctgctcca gtggcaagtg cagcagcaga agtagctcca
     2821 aaagttgaag aaaagaaact tttaagaagc ttaactaaaa aacactttaa aatagataaa
     2881 gttgaatttg ctgatgaaac taaaatagaa ggaactactt tatacatcag aaacgcagaa
     2941 gaaatatgta aagaagctaa tgaaactcaa gagttagttg tagatatgaa gttagaaata
     3001 ataactcctg ataaatatga aacttacagt gaagctgtat tagatataca accaatcgct
     3061 actaaagaag aaggcgaatt aggttcaggt ataactagag ttatagatgg agctgtaatg
     3121 gtattaactg gtacagatga agatggagtt caaataggtg aatttggttc ttcagaaggt
     3181 gagttaaata ctactataat gtggggtaga ccaggtgctg ctgacaaagg tgaaatattc
     3241 atcaaaggtc aagtaacaat aaaagcagga actaacatgg aaagaccagg acctttagct
     3301 gctcaccgtg catttgacta tgtaactcaa gaaataagag aagcattaaa gaaagttgac
     3361 aactctttag tagttgatga agaagtaata gagcaataca gaagagaagg taaaaagaaa
     3421 gttgttgtta taaaagaaat aatgggacaa ggtgcaatgc atgataacct aatattacca
     3481 gttgaaccag ttggtacatt aggagctcaa ccaaacgttg acttaggaaa catgccagtt
     3541 gtattatctc cacttgaagt attagatggt ggtatccatg cattaacttg tataggacct
     3601 gcatcaaaag aaatgtcaag acattactgg agagagccat tagtaataag agctatggaa
     3661 gacgaagaaa tagatttagt aggtgttgta tttgttggtt ctccacaagt aaatgctgag
     3721 aaattctatg tatctaagag attaggtatg ttagttgaag ctatggaagt tgatggagct
     3781 gtagtaacta ctgaaggttt cggaaacaac catatagatt tcgcatctca catagagcaa
     3841 ataggtatga gaggtatacc agtagttggt gtaagtttct cagctgttca aggtgctcta
     3901 gttgttggta ataaatacat gactcacatg gtagacaaca ataagtctaa acaaggtata
     3961 gagaatgaaa tattatctaa caacacttta gctccagaag atgctgttag aataatggct
     4021 atgcttaaaa atgctatcga aggtgtagaa gttaaagctc ctgaaagaaa atggaatcca
     4081 aatgttaaat taaataacat agaagctata gaaaaagtta caggagaaaa aatagtatta
     4141 gaagagaatg agcaatctct accaatgagt aagaagagaa gagaaatata cgaaaaagac
     4201 gaaaactaaa atttagatac taactaagat ttagacaata acataaattt agatccaagt
     4261 ttaaaacagt atagagaggt agaattataa tggataggtt aaaatatatc tcatcagaga
     4321 ctttttatga aggtattata gttgacatca aaggtggtgg agtgactata gacctaaaag
     4381 gtagacttgg acaatttaaa atacctaata gaatgcttat aactgactac gaacttaaga
     4441 taggtcaaga agttggattc atgctaagct atcctgaggt attaagtcca gagcctaatc
     4501 aagagtatgt agaaaatatt aggagagaaa aagaaaaaca agcagaaatg caatcaaaac
     4561 aagaagaaaa ataataaata atcgtttgaa aatagaaggg agaggaaata tatgagcctt
     4621 acaacagtac aaggacttca atctgaaata ttcgttccaa taacacctcc tccagtttgg
     4681 actcctgtaa ctaaagaatt aaaagatatg actatagctt tagctacagc agctggtgtt
     4741 catttaaaat ctgacaagag atttaatcta gcaggagata ctacatttag agctatacct
     4801 aatacagcta cagttgatga aatgatggta tcacatggtg gatatgacaa tggagatgta
     4861 aacaaagata taaactgtat gttccctata gatagattac atgaattagc tgcagaagga
     4921 tttatcaaag gagtagctcc tatgcactat gcattcatgg gtggtggagg aaaccaacat
     4981 gtcttcactg aagaaactgg tcctgctatc gctgctaaac ttaaggaaga gggagtagac
     5041 ggtgtagtta tgacagctgg ctgaggtact tgccatagaa ctgccgtgat cgtgcagaga
     5101 gcaatagagg aagctggaat acctacaata ataatcgcag ctcttcctcc agtagttaga
     5161 caaaacggaa ctcctagagc agttgctcca ttagttccaa tgggtgctaa tgctggtgaa
     5221 ccacataata tagaaatgca aacacatata ttaagagata cattagagca attagttgca
     5281 ataccatctg ctggtaagat agttccatta ccatacgaat ataaagctca cgtttaaaat
     5341 gtaatttata tttgaaaata tagaaccctt ctcctacaca gttaggagag gggttttaag
     5401 aacaaaggtg gatattatgg aagaaaaaat aatgagaaga cttgttataa aaccgtttca
     5461 tatgaatgaa gttaatttcg gttcaaaaac ttctataaag aaagatgtac ttacaataga
     5521 tttatcaagt atagatgaaa taaaagaaag agaagagtta ataacagaca taaaagtaga
     5581 cattataaaa cctggagatt atgacagaga aattaatact attatggaca ttataccaat
     5641 atcaacaaaa gttttaggca gactgggaga gggtattaca catactttaa cgggtgtata
     5701 tgtaatgtta actggtgcag atgaagatgg aaatcaaatg catgaatttg gttcttcaga
     5761 aggaattttg aaagaccaaa tggtatttgg gagatatgga actccatcag ttgaagatta
     5821 tataatccat gtagatgtaa ctttaaaggg aggtttacct tttgaaagaa cacttccact
     5881 tgcagcattc agagcatgtg atgactttat acaagaaata agagcgtctt tgaaaatgga
     5941 agacggaaga aatgctactc aagttcgtga atattttgac aaaattcgac caaatgcaaa
     6001 aaaggttgtc atagtaaaac aaatagcagg tcaaggtgct atgtatgaca atcagttatt
     6061 ctcaaaggaa cctagtggat ttgaaggagg aacatcaatt atagatatgg gaaatgtacc
     6121 tatgattata tctcctaatg aatacagaga tggtgccctt agagctatga cttaagagag
     6181 gtggatatta tgggaatagg accttcaaca aaagaaacat ctcttcatca ctttagagac
     6241 ccacttttag acatagttgg aagtgataag gatgtagatt tacttggagt aatagtagta
     6301 ggaactccag atggaaatga aaataaaacc tttgtgggac aaagaacggc cgcttggctg
     6361 gaagctatga gggtagatgg agctatagtt tcatcagatg gttggggaaa ttcacatgtt
     6421 gactacgcta acacatttga ggaaatagga aaaagagata tacctgtagt tggagttaca
     6481 tttaatggga ctcaagctaa gtttgttgta agcaatcaat atatggatac catagttgat
     6541 atgaacaaat caaaagaagg tattgagaca gaagtagttg gagaaaacaa cacaaatgaa
     6601 atagatgcta aaaaagcttt ggcattttta aaacttaaaa tgagaaaaca tggataaaaa
     6661 atttaagtaa gggaatgagt tttgtgggaa tttctacatc aatgaaaaac acatcacttc
     6721 atatatttag agacccatta ctagatttga tagataatga ttctgacata gacttgttag
     6781 gagtggttgt agttgggaca tcagagcaca atgaatggaa aacattttta gcaactagac
     6841 ttggaagatg gattgaatca ttgcgacctg atggagtgat tataacttta gatatggcag
     6901 gaaaccagca cattgatttt acaaatgcta tagcagagtt tgtaaaatca gatataccta
     6961 cagtaggtct tactattatg ggagcagatg gattagtaat taccaatcca tatttggata
     7021 aagctaccat aattgattat aaaaagacaa caggctacat agaaacagaa gtagttggag
     7081 acaatcacat ggatgaagtt gatgttaaaa aagcattggc tttcctaaaa ttaaaaatgc
     7141 gtaaagacgc aaaataaacc taagggggaa tttgatatga aatttagcag aagtatacaa
     7201 gctatagact ctcatacagc aggtgaagca actagaatag ttgtaggtgg aatacctaac
     7261 ataaaaggaa attctatgcc tgagaaaaaa gaatatttag aggaaaatct agattattta
     7321 agaactgcta taatgttaga gccaagagga cataatgata tgtttggttc tgtaatgact
     7381 caaccttgtt gcccagatgc tgactttgga ataatcttca tggatggtgg cggatacctt
     7441 aacatgtgtg gccatggtac aataggagct atgacagcag ctatagaaac aggtgtagtt
     7501 ccagcagtag agcctgtaac tcatgtagtt atggaagctc cagcaggaat aataagagga
     7561 gacgttacag ttgtagatgg aaaagctaaa gaagtatcat tcttaaacgt accagctttc
     7621 ttatataaag aaggtgttga agttgactta ccaggtgttg gaactgttaa atttgatata
     7681 tcatttggtg gaagcttctt tgctataata catgcaagtc aattaggttt aaaaatcgaa
     7741 cctcaaaatg ctggtaaatt aactgaacta gctatgaaac ttagagatat aataaatgag
     7801 aaaatagaaa tacaacatcc aactttagct catataaaaa ctgtagactt agttgaaata
     7861 tatgatgagc caactcatcc agaagctact tacaaaaacg tagttatatt tggtcaaggt
     7921 caagttgaca gatctccatg tggaactgga acaagtgcta aattagctac tttacatgct
     7981 aaaggtgaat taaaagtagg agaaaaattc gtatatgaaa gtatattagg aactttattc
     8041 aaaggtgaaa tagttgaaga aactaaagtt gcagatttca atgctgtagt acctaaaata
     8101 actggttctg cttatataac tggatttaat cattttgtaa tagatgaaga agacccactt
     8161 aaacatggat ttattcttaa ataagtaata aataaattgg gaaattaaaa tagaatagta
     8221 gccatttatc ggctactatt ctatagcttt taggctataa gattcagaat atatgaaaaa
     8281 ataaaaaaat aggaggatgg attatggtag caatcttaaa ggcattatat gtagtattcg
     8341 cagcaatctt tgcagttgtg tttggtaaag acgtagctaa agcaaacaaa gaggggaaac
     8401 tagaagaaca aagtactcca aaagttgctg taacaggatt tataacagac ttctttgata
     8461 cattaggaat agggtcattt gctccaacaa tagcaatgtc aaaggcttta aagttaaata
     8521 taccagataa aaaaatgcct ggtacattaa acgtagcaca tactattcca gttgttacag
     8581 aagcattcat atttacatca atcataccag ttgatggtgt tactttagta tctatggttg
     8641 tggcagcagc agttggttca tatataggtg caggaataat agctaagatg gacgagagaa
     8701 aaatacaact tgttatgggt gtaactttag cattaacagc aatattaatg ttattaggtc
     8761 atccttggat taacgtatta ccaggtggag gaaatgaatt aggtcttact ggtgctaaat
     8821 tagtaatagg tgtaataggt aactttatac taggagcttt aatgacagct ggtataggtc
     8881 tatatgctcc aggtatggca atggtttatt tcttaggaat gtctgcaaaa gttgcattcc
     8941 caatagttat gggttcttgt gcattattaa tgccagttgc aagtatgaaa ttcataaaag
     9001 aagatgcata tactaaaaaa gcatcagtta taatagctat atgtggttta ataggagttt
     9061 tcgtagctgc atatatagta aaaactctac caatggatat attaaaagca ttagtaatag
     9121 tagttatagc ttatacttct gctacaatga taatggctgc aaataaaaac aaaaagactg
     9181 cttagttaaa gactacttag tgtttattaa atattacagc taataataaa gattataaac
     9241 tgctttaaaa tattgaatta atattttagg gcagtttttt atttacta
//
LOCUS       DAEMAE010000144         9115 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEMAE010000144 DAEMAE010000000
VERSION     DAEMAE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.146"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..554
                     /locus_tag="KPH93_002650"
     CDS             <1..554
                     /locus_tag="KPH93_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1689456.1"
                     /translation="SSIIMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIF
                     YNDVNMEISKSIRHNTDFSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNE
                     DIIYSLQKDMIGILMPNTSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQ
                     YKSSFGDSINFKNTVEEELQYDV"
     gene            544..2208
                     /locus_tag="KPH93_002651"
     CDS             544..2208
                     /locus_tag="KPH93_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1689457.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YDNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTNNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIKNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            2257..3513
                     /locus_tag="KPH93_002652"
     CDS             2257..3513
                     /locus_tag="KPH93_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1689458.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFALSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            3536..5617
                     /locus_tag="KPH93_002653"
     CDS             3536..5617
                     /locus_tag="KPH93_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG1689459.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYVIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKMGNSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSTKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVFVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSD
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVIIL
                     SMLYIKKYKRR"
     gene            5654..6739
                     /locus_tag="KPH93_002654"
     CDS             5654..6739
                     /locus_tag="KPH93_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG1689460.1"
                     /translation="MRKYKNKFKLKRSILISVIILIFIFIIYFVSIELKDMSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKAKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNTILNELIIDVANPL
                     DVNVLNIGSNFVHLEGYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKSVNTVDELISALHSSTVNNREQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHAPSKVVNGEEQARCFEAYKQVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            6793..7917
                     /gene="wecB"
                     /locus_tag="KPH93_002655"
     CDS             6793..7917
                     /gene="wecB"
                     /locus_tag="KPH93_002655"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG1689461.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLAKKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPKKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            7993..8967
                     /locus_tag="KPH93_002656"
     CDS             7993..8967
                     /locus_tag="KPH93_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG1689462.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     NIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKQLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         3843 a          845 c         1475 g         2952 t
ORIGIN      
        1 tatcttcaat tataatgggt aatctgaaca aaagtataaa tgaattgcaa aatacaaata
       61 aaaaactatc tgaacaatat aaggaattag tgactattga tagtgaaact ggacttagaa
      121 atttaaagat tttttataat gatgtaaata tggagataag taaatctatt agacataata
      181 ctgatttttc tcttatgata gtaaaattac cgtactacgg aaatctacag actatatttg
      241 gtgaaaacaa aactaataaa atagtaaaat atattggttc aaatattata gaatgcacaa
      301 gaaatgaaga tataatctat agtttacaaa aagatatgat agggatacta atgccaaata
      361 catctttgga aggttcaaaa gttgttaaag acagaataaa gaaaaggata aaagaattaa
      421 atttagattt aaacaatagg ggaaaatatg taaatataga tgtcaaaatt gcatttttac
      481 aatataagag tagttttgga gatagtataa attttaagaa tacagttgaa gaggaacttc
      541 aatatgatgt ttaaaatatt caataaaagt ttattaaaat tagtagtcgt attatcaata
      601 gtactgacaa tatcaaattt tagcttatct tcatatgcat cagaaattac ttccagcaca
      661 gataaggtat taatcatata tgattctaag aaagaaacag cttacaatag agatatttta
      721 aatattatga ggactctatt gggaagattt agttcagata tagagctatt aaaattgagt
      781 aactatgatg gtgagataaa taaaaattat tacagtcata tatttatttt aggaatcaat
      841 gaaaactcat atgataatga taaaaacact aaaaacttga ttagttcctt aaattcatat
      901 aaaggaacta tatgttggtt aggatatggt atagaaaatc ttttggaaca taaaaaatac
      961 aatttagact atgtaggaaa aacaaacaat atagttagtg taaattatag aggtaaatca
     1021 tataacttgg atgaacatta tgtttttaac attgtagaat caaaagatac aaataataaa
     1081 gtaataggaa gtattaatga taccttaaat aagtatccat atataataaa tgataaaaat
     1141 cttttttatg tatcaaaatt agatttagat ggagttttat tttatatatt ttgcgattca
     1201 cttaacgata tttttaatat taagacgttt gataaaggac gtatatttgt aagaatagaa
     1261 gatgtacatg cttttagaga accaaaaaat cttgtagaga tagcggatta tttaagtagt
     1321 aagaatatac catttacgat tgcattaata ccagcatatg taaatcctaa aaatcataaa
     1381 gtaataactt tatcagaaag ccctgaaatt gtcaaagcaa taaaatatat gcaagataaa
     1441 ggtggaactg tgatattgca tggatatact catcaataca agaaagaaga agtttctgga
     1501 gaagggtatg aattttggga tggtaaaaaa gatgaaccct taaaagaaaa tatgaagata
     1561 tttgtcaaag atagagtttt gaatggtctt agagtatgta tagagaatgg aatttatcct
     1621 cttgcatttg aagcgccaca ttatgcaatg gaatcagagg gatataaaga attaaaaaaa
     1681 tatttttcta cttatatggg gcaacatcaa aataatgata aaaaatttag cacaaataca
     1741 tatccttata ttattaggga tacagaggag tttaatatat ttatacccga aaatttagga
     1801 tatatagacc cagaagataa gtttactttt caaaatataa aagaaaattt agataaatta
     1861 tccattgtaa gaggttttag tggagggttc ttctttcatt catatttaaa tatagagtat
     1921 ctaaaaaaca caatagaata tttagaaaaa cagaatatag aatttatgaa tttaagagat
     1981 tttaataatt gggtaaaagt tgatgaaata caaataaaaa ataatggaga tgaaattata
     2041 gtcaactatg ataaggactt agatgaaata actaaaagtg atactagatt taaatccata
     2101 tccaatatta gtaaaatttt aatattcata gtatctataa gtgttttaat atttgtgatt
     2161 atatttatat attttaagag aatagataaa aagaaatttt taaaataaat gattttatat
     2221 tagaatcaat attttaaaat attttaggag aaatttatga gagatttaaa tgtatcagag
     2281 tatttgttcg tatttagttt gtttagtata tggtctttat tgcttataaa tattatttta
     2341 gctatgggtg gatatatatt ttattttaaa aactttgata aagaaataaa agaaattgat
     2401 gaatatccta tgatatcaat acttgttcct gctcataatg aagctaaagt gataggtaga
     2461 actgtggagt ctttgctatt actcaattat cctaagagta agatggaact tattgttata
     2521 aatgacaatt cttctgataa tagtaaagaa attttagaaa atataaagga taggtacaac
     2581 aattataatt tcactataat aaatacagat agtttaacgg gtggaaaagg aaaatctaac
     2641 gcattaaata ttggatatac gatttcaaaa ggagatttta tagctgttta tgatgcagat
     2701 aacacaccag ataaaaatgc acttagatat ttagtacaaa ctatagtaat gaatgatgaa
     2761 ttaggtgctg ttattgggaa atttaggact cgaaataaaa ataaaaacct attaaccaaa
     2821 tttataaaca tagaaacact aagctttcaa tggatgtcac aagctggtag atggcaactt
     2881 tttaatctat gtacaatacc aggaactaac tttatattga ggagaagtat catagaagaa
     2941 attggaggat gggatagtaa agcaattgca gaagatacag aaataagttt taggatatat
     3001 aaattgggat acaagataaa gcttgtacca caatctataa catgggaaca agagccagaa
     3061 actgttaagg tgtggataaa gcaacgtact agatgggcaa aagggaatat atatgtatta
     3121 atgaaatata taaaaaatat atttaaacag ggaagaaata agattgtatt tgatatagct
     3181 tacttttttt ctgtatattt tctattttta acatctgtca ttatatccga tattttattt
     3241 gctttaagta tatctaaact agtagaaatt tctattccaa ttaacttttt tctaatatgg
     3301 atactatctt atcttttatt tattatagaa gtgagtataa gccttactat agaaaaaggt
     3361 gaagcaacaa ttgaaaatat attcatagtt gcaataatgt attttacata ctctcaacta
     3421 tggctgtttg tagctataaa aggaatgata gagtatctaa aagatataat ttttaaaaga
     3481 gaggttaaat ggtataaaac agaaagattt tgattcatgt acaggaggaa ttaacatgaa
     3541 aaaatttatt atatcaatta tatctttagt attgtttttt tcaaatatat cacttatata
     3601 caaagtaaat gctgacgaga ctaaagttaa aaattataag tttgaaagag atataactat
     3661 tgatggtgtt ataggaagta atagtacttt ttttgaagta aataaaaatt gggatattga
     3721 agaagtttta ttacatctta attttagtaa gagtcaaata ttaaatggag atgtttcaag
     3781 tttaactgtt ttgataaata atgtaccaat aaaatctatt aaattaaatg ctaagacaaa
     3841 ttataaaaat acactagaag tattagttcc aaaagattat gtaattcaag gatataacga
     3901 aataaaaata aaaacataca aaacaatatc tgataaaatt tgccaagatg attccaatac
     3961 tgggaactgg atggttattc acaaagaatc atatatatca attagatata aacagaaaaa
     4021 gatgggaaat tcaataaatg aatatccgta tccatatgca gaaattgaaa ataatcataa
     4081 attagataca actatagtag taccagataa tatgactaga ggagagacta cagcagtgtt
     4141 taatttggca tcagcatttg gaaaaataac aaaaaatgat gatttaaagt tagatgttaa
     4201 attatattca gaaatgaaaa attggtcaga tgataatatt atatatattg gtaaacctga
     4261 aaatacagca gaagaaattt tggatatttt aagtacaaag gaacaaaccc tattatcttc
     4321 aaactgtata atcaagcaag tagattcacc atacaataag aataagaaga tgatggtggt
     4381 aattggtagt aatgaagatg atttaataaa ggcatcaaat ttattaatag aaaatagact
     4441 ttctaatcaa gttttatcat catcagtatt tgtaaacaaa gagactaata taaaaatcaa
     4501 tagggaacaa aaattaaatt taggtcattt aacattaaaa gacttaggat attctgactt
     4561 tttgttggaa ggagcattta atcaacaagc tttgtttgat gtcaaaatac caacgggtaa
     4621 ggtgttagat gatggttcaa agattatctt aaatttaaga tattctgaca atttagattt
     4681 tgaaaaatct ttagttacag tgtctataaa tgatgttatt gtgggaagta aaaaattgga
     4741 taggtcacat tctaataatg ataagttaga attaaaaata cctaaagata tagataataa
     4801 aaactattat caagttaaat tgacatttaa tttgagtata aaaaattcga attgtgttac
     4861 tagagagagt aataatcctt gggcatatgt atcaaataat tcttatttgg cgttatctac
     4921 gaaagaaaat gaaaccttat cttttgaaaa ttatccgtat ccatttgtta gagatgatga
     4981 atttaatgat ttaactgtga taatgccaga ttattcaggg tcacaagcta tgacctggat
     5041 gtttagatta ggtgttactt taggtgcgaa tataaattca cataatggaa atataaatgt
     5101 aattagagga aaagaattta gtgacaagta taaggatact aatattgtag tttttggagt
     5161 accacataat aattctgtta taaagatgtt aaataataat ttaaatataa agttcgataa
     5221 aaattactcc gattttatat caaatgataa gataagcttt attgatgatt atggaaaaaa
     5281 tatttctacc attcaattaa taaaatcacc atataataat caaaaaaata tcatggtaat
     5341 tagttcaatg aatgaaaaaa acttatattt aggaatggat tatttgttga ataaaagtaa
     5401 agttaatgat ttaaaaggag atacattgat aattgatgaa tacggagagg tagaagattt
     5461 agcttataat ttaaaatcta agaaagaagt aaaagattca agctggaata tgagtataaa
     5521 taagacaact aaagtatttt taatgatttc ttttattact ataattgtag tcataatatt
     5581 atcaatgcta tatataaaaa aatataaaag aagataatat gataaattta ttattttaaa
     5641 agaggtatat aatttgagga aatataagaa taaatttaaa ttaaaaagaa gcattttaat
     5701 ctcagtaata atcttaattt ttatatttat aatttacttt gtaagtattg agctaaaaga
     5761 tatgagtcta gtaaataatc aatataaagg taagattaaa tcggccaatt tatctgtaga
     5821 ttatgaaata aatcaagtta tgaaggatat agataaattg ggattgaata cagtaaatgt
     5881 acctataatt ataaatgtag agtccattaa ttctgatatt atgtcaatag ataataatag
     5941 taaagcaaag gcaattaagt taataaaaaa attaaataaa aaaggtattt caacaatatt
     6001 agaggcatat ccatggattg aaaatggaaa attgtatgag actgactgga accctataaa
     6061 taaaaaaaga tttttttata cttggcaaaa tactatatta aatgaactta taattgatgt
     6121 agcaaatcca ctggatgtaa atgttttaaa tattggttct aattttgtac atttggaagg
     6181 atatcaacaa aattggggtg aaataataga ttttgtacaa tccaaatttg atggtcttgt
     6241 aacatacaga actaattggt ggtatacaaa aaaagatgac ttaagtagta agttgtttta
     6301 tgaacaaaag ttgaacaata atttttttga taagttagat tttatatcta tagcagcata
     6361 ttttgagtta agtaataaat ctgttaatac agtagatgaa ttaatttctg ccttacactc
     6421 atctactgta aacaatagag aacaaaatat aaaacaagaa atatataacc tttataaagc
     6481 tcatagaaaa cctatatttt ttggagaatt aggtttttca aatagagaaa gtgcaagttc
     6541 tcagccgtgg aatcatgctc cttcaaaagt tgtaaatggc gaagaacaag caaggtgttt
     6601 tgaggcatat aagcaagttt ttgaaaatga agactggata aatggttttt ctgtgttttg
     6661 tgtaggtaaa atagatgacg aaaaaaattt ttacccatca aaagagagta taaaagttat
     6721 taagtcttgg tatgaataat tttgaaagtg tagaatttat atagattaaa tagacgatta
     6781 ggagagatat aaatgaatga catcaaggtt atgactgtat ttggaactag acctgaagct
     6841 atcaaagtag cacctttaat aaaggaactt gaaaaaagag agaatataaa aagtattgtt
     6901 tgcgttacag ctcaacatag agaaatgctt gaccaagtta tagaaacctt taatataaat
     6961 gtagactatg atttggatat aatggaaaaa ggacaaagcc tgaatgatat aacatgtaag
     7021 attttaaata aacttccctt aattttaaac aaggaaaatc caaatataat attggtacat
     7081 ggagatacaa ccacaactct agctacaagt ttgactgcat tttataataa aactcttgtt
     7141 ggtcatatag aagctgggct tagaacctat gataaatatt ctccttttcc agaagagtta
     7201 aatagacaac ttacaggtat tattgctgac atgcactttg ctcctactaa tttggctaaa
     7261 aaaaacttga tttctgaagg aaaacctaat aataatatat ttgtaactgg taatactgct
     7321 attgatgctt taaagatgac aataaaagaa aactataatc atcctataat tgatgagatt
     7381 ggcaatgata ggatgatttt gttaacctct cacagaagag aaaatttggg taaacctatg
     7441 aaaaatatat tcagagctat aaagcgaata gtagatgatt ttgaagatgt acagatagta
     7501 tatcccatac atttaaatcc taaaatacga actatagctg atgagatatt tggaaaattt
     7561 cctaaaaaaa ttcatattat agagccttta gatgtagctg attttcataa ctttttaaat
     7621 aaatcttata tgataatgac tgatagtgga ggaatacagg aagaagctcc atcattaggt
     7681 aaaccagttc ttgtgttgcg tgataaaaca gaaagaacag aaggaattga agcaaagaca
     7741 cttaaattag taggtactaa tgaagataga atatataatt cagtgagtga tttattaata
     7801 aataaagata actatgttca gatgagtaaa gcatctaatc catatggaga tggaaatgca
     7861 agcaaatata tagtggatat aataattaaa aaatttaatt gtaaatattt aaattagata
     7921 aggtctaaaa cattaactaa tgatataaga tttatgataa tataaggaat acaattttta
     7981 agggggagta atatggctag aaaattaata aaaaatttag gtaaaagtaa aagtgtaaag
     8041 agagtgaaac ttttatttaa gaagattttt attactgtat tcatagtagc aagtatagtt
     8101 gctattttta atataactaa atattttgag gaattataca aagtaaggga tttaaaaagt
     8161 acaaaaattg aatactatat ggatgtggct gatgaagcag gggatggaaa ggtccaatta
     8221 agttggaagg ccttacttgc tatagacatg gtaatacatg atgaagattt aagtaatata
     8281 aaaaagaaag acacattgaa tataggggaa aagtttatag tagaagataa aaatgataaa
     8341 ggcgaaaagg tgtataaggt aaaaaagttt aataaggtat taagcgaatt gaaatttgac
     8401 tcttctcaaa aaagtagagc aagaaaatac atgaaagatt tggaatacac atatcttgga
     8461 aataaacaat tagatagtag tgatgaaaaa attaaattta taaagcagtt agaagactca
     8521 gctataagag aatatattga ttatggaata ttgccatcta taacaattgg acaagctata
     8581 ttagaatctg gctggggaaa ttctaaactt acaaaacaga gtaataattt atttggtata
     8641 aaagcagata aagcatggaa aggaaaaagt gtagaaattt caacttcaga gcattataat
     8701 gaaaaaattg tagctagttt taggtcttat aattcattac aagattctgt caaagaccac
     8761 agtttatttt taattaataa taaaagatat agaaaacatg ggttgtttga ggcaaaagat
     8821 tatattagtc aagctcaagc attagaaaat gcaggatata gtacagcaga agataaaaaa
     8881 ggaaatcgta tatatgcaga gctattaata gatgttatta ggagttataa tttgcaattg
     8941 atagacaata aagttgagac aaaataattg ttaactttat atgaggagat aacaagataa
     9001 gattattttc tacaaaaaga atagaaaaaa tgtaaaaaaa actaaaaaaa tgtattgact
     9061 tgttatttaa aaggtgatat tattattact gtactcgaaa gagaacaaaa tgaac
//
LOCUS       DAEMAE010000145         9098 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEMAE010000145 DAEMAE010000000
VERSION     DAEMAE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.147"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            472..1875
                     /locus_tag="KPH93_002657"
     CDS             472..1875
                     /locus_tag="KPH93_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1689463.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEVCYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2282..3304)
                     /locus_tag="KPH93_002658"
     CDS             complement(2282..3304)
                     /locus_tag="KPH93_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1689464.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3339..4937)
                     /locus_tag="KPH93_002659"
     CDS             complement(3339..4937)
                     /locus_tag="KPH93_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG1689465.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSIVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNVDDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4979..6244)
                     /locus_tag="KPH93_002660"
     CDS             complement(4979..6244)
                     /locus_tag="KPH93_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1689466.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLRQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAVLT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPVVGAILAILFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6389..7801)
                     /locus_tag="KPH93_002661"
     CDS             complement(6389..7801)
                     /locus_tag="KPH93_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG1689467.1"
                     /translation="MASTNKNNVTSKGEEKEASTSGLESCTVKENKAKTNQTNFSNIN
                     ENQTNENKTNTYKGDIDKGNIDKTNTNKSDINKVNIDTMDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            complement(7839..8543)
                     /locus_tag="KPH93_002662"
                     /pseudo
     CDS             complement(7839..8543)
                     /locus_tag="KPH93_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(8661..>9098)
                     /locus_tag="KPH93_002663"
     CDS             complement(8661..>9098)
                     /locus_tag="KPH93_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1689468.1"
                     /translation="LNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDL
                     QVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKN
                     EEVKNLNIREWICPSCNETHNRDINASINILKEGLRLITIQNK"
BASE COUNT         3151 a         1669 c         1093 g         3185 t
ORIGIN      
        1 ttatattcta atattctact atactacaca acaaacccta tattatattc taatatgcta
       61 ctatactaca caacaaatcc tacattatat tctaatatac tacccactat tttgcgctac
      121 aacaatttct ctatttatat gatttgtaaa atatagtaca acatttttta tcatttttat
      181 tccagctaaa tttgtaatat tttattcaat gcataatcaa taattaatca atacatgctt
      241 tataatacct tactcaatat ataatcaata cataatttat aatttgtaat actttattca
      301 atacataaat agaaatctct atatacataa attcactttt tcatacatct tacaaattta
      361 tttttattta gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa
      421 tatggaaaat ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg
      481 ctaaacaact tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg
      541 gaaagagata atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac
      601 gttgctggat tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca
      661 ccacaaaaat atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct
      721 ccaggaacag gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt
      781 ttcaatgtta ccggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt
      841 agaactctat ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata
      901 gatgctgtag gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac
      961 caacttttag ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct
     1021 accaatagac ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac
     1081 attcatatag gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat
     1141 gacaaaccta tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc
     1201 aatggggctc atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac
     1261 agtgagtgta ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta
     1321 gaatctaaaa actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct
     1381 ggacatgctc ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt
     1441 cctagaggac aagcattagg ttatgttctt caattacctg atgaagatag atatatttat
     1501 actaaggacg agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagag
     1561 ttaatattta atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata
     1621 gctaaccaaa tggtttgtga atatggtatg agtaatttag gatttatgac tattgatgga
     1681 aatgataaga ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaagtt
     1741 tgttataaag agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct
     1801 aaattcttat ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa
     1861 gaagctgtaa actagtatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct
     1921 ctttttattg ttaaagttat aaaaatatat ttgatttcat acaagaaagt gcctcataaa
     1981 ctaaaaaatt cattttgaga cacttccata ttttaatttt gtctatttgc ataatattat
     2041 atctacataa aactaaactg ctgtaaactt aactacctcc agtaatgttt gaagtggtat
     2101 aaaagctagt agcctagttt ctacacctat aatttatatt ttacataact aaatttagta
     2161 gatttttata ctcacaattt agaacaattg gaaacttaaa aatttcttat cagacatcta
     2221 caacttaatt aggtatctat cacttgatta aacatttatc acttaattaa atatctatca
     2281 cttaaagtgt agaacctctt tcaatcaatt ccggttttaa catttcaaca actgactctg
     2341 gattttcttc atttaataag gacttaagta tgttaatggt tttttcaatc aacaaatcta
     2401 gtggttgtct aatcgtcgtt aaattataac tcttccatga agccatttga atatcatcat
     2461 atcctatcac cgataaatcc tctggcaccc tcaacttata ctcaaacctt gcagcatcca
     2521 ttacaccaat agctatcaag tcacttgtgc aaaaaattgc tgttggagga tttgataatg
     2581 ctaacattct cttacctgct tcatatccag aatcataatc atagtttgca gattcttctt
     2641 taagattatg aatcccactt tgtcttagct tagaatagaa tccaattttc ttttccattt
     2701 cctcacttgt ttctctcgta aattgaatat atcctatatt cttgtgacct ttcttaagca
     2761 gatattcagc agccattccc ccaccttcaa ttgggtctac atagactgta cttattttta
     2821 taccaggtat aaaacgatta aaaagaatca caggaatatt attttcttca caagtttcag
     2881 ccatcacctt attcatggct gaagctgtaa taatcacagc ttctacttga aactgaatta
     2941 cttttgaaat tatactatct atactttgtt gtctcggaac tttaaaaaca agacactgtt
     3001 tacctatctc ttgaattttt tctacaaata aattgataat tctattgtaa aatggaccta
     3061 gactgtcacc aactatcaat cctataacat tagtttttcc actaatcatt ccccgtgcaa
     3121 ccaaatttgg aacatatcct atttcatttg ctacacgaaa aattctttct cttacttcag
     3181 gttttacgcc ctttccattt gcactaccaa atgctcttga aacagatgat tgagatactc
     3241 ctgccaatct tgcaacatct tttgatgtag ccgatttatt ataatgaact ttcttttgat
     3301 tcatttagta tttctcctta aatatctagt atttcttctt aaaattgtat caaaaataat
     3361 ataatatatt ggataataaa aaaagctaat acaattaatt cttgtttacc taaagaaaat
     3421 ggcactatag atactccatc tactttatcc attccttgag aaataaggtg atttctcata
     3481 ccaaatgcag tgtatttttt aatatcatct acattatgat ttttctttaa aatgacacta
     3541 aaaatatatc caaatatcaa tgttattgta gcacctattg ctggattcca caaccaactc
     3601 acattatatt tattagaaat aaaaaatcct aaaactaacc ctacaataaa accaatagat
     3661 actcctttat cattagcttt atatgtaaac attgctagta caaatgcacc tgtagctggt
     3721 ccagcaaagt atgagatata gttaccaaca aggtcaagaa ttgatttaat gcttcctcca
     3781 aatcccataa taataaaacc tgtcataaca actcctataa ctacagttat aatcattgta
     3841 acttttaatg atgattcttt gttatctttt ttgaaataaa actcataaat atccttcgta
     3901 aaaacagttg tcatactgtt taatatagag tcgatactag acattgctgc tgcaaacaca
     3961 cctgcaataa taattccaat ggctcctact ggaagttcat ttaaaataaa agtaatcatt
     4021 atttcatttg aagtttcaaa tgtccttccc ccgtaaaaaa caaataaaat tactcctacc
     4081 aatagcataa agtagtaaac taaattcata acaaatgcac taactacaaa agaatttttt
     4141 gcacatttta aagatttaga tgttaaaact ctttgtactt gagattggtc aaaacagaag
     4201 tatcttatcc acattacagt tccaccaatt aaagtagccc aaaaagtatt tgtaactgaa
     4261 atatccctac tgaaatcaaa tgtatttaat tttccactct gttgtgcaat ttgtaatgta
     4321 tcaaagaaac ttagattcat tccttttaag gctacaataa ttactataaa tacagcactt
     4381 attactacta tcatttgaat gctatcagtc catataactg ccttaatccc acctaataga
     4441 gtatatataa ttgatacaag tacaactatt gggactaaag cataaagtga ccagccagta
     4501 atcatttgaa taataagcac tggaatatat actatagaac tagcttgaat caatgaatta
     4561 ataaaaaatt gaataatagt taatcctctc gaataatttc ctaaacgaag tcccatatat
     4621 tcatatatag aggttatttt taatttatag ataactgggg tggtaatgct cagtgcaaaa
     4681 aatatcgcta aaggtactgt aatattaacc attactggat acattccatc tacatatgcc
     4741 catccaggac cacctataaa accatttgca ctaatcatag tagctgcaac tgataaagca
     4801 attggaatcc atggcattga cctacctgcc aagaaataat cttcttgatt tttattatct
     4861 tttcctgaat aataaccaat aactaacatt aatagcaaat atcctattat aatacataca
     4921 tcaattgatg aaatcataag ccaccccttt ctaagtaaca aaataaattc ctagtatttt
     4981 atttcttatt atttaacgtc tctggtaaga ataaccacac aatagcacct gctacaaaga
     5041 ctgctattga tatgtaaaag atagtcatca tacctaaagt accagaagct gtaccagcta
     5101 tagcaggtat tgcaaatgta gatataatcc ttcctccatt aaatgacata ccagatgaca
     5161 acgaacgata tttacttggg aacaactctg tgtagtatgc tccccatgct ccagaatatc
     5221 caacagcaac acctagtaat actgatatca tgtagaatgt tgttgtatta tctccaccta
     5281 attttgtata tataaaaaca aatactgctg ctaatgccat acctataaaa ggtatttttc
     5341 ttccaaattt atctgcaatt gaaccccaga ataaatatcc tactaattga ccaattccct
     5401 gtaaactata tatagaacca gccactgctg ctgacattcc tattcctgtc actaggtatg
     5461 tagttgcatt atttgaaaat gctgaatatg ctgtaaaatt tgcacctgat aaaagaatcg
     5521 ttcccaatcc aatccataag tattttccct tatacatact tatatagcta atcttttcac
     5581 cagtttcttt tcccttttta actaattcct ttgtatactc caaatcatct ggaacaaata
     5641 aaattgctaa gatagctcca actacaggtg gtataatcgc agtatacatc agtatttgcc
     5701 aatttgcatc acctaaaacg ccatatactt tcccaccaaa aatcatggct aatgaaaata
     5761 aagcagttaa tacagcagct aacttaccac gatatttact tggaaacata tctgcaacta
     5821 aaggcatagc tgttccaaac acgcccccta acactactcc tgtacacaaa cgtgctattt
     5881 tccataacat aatatttgta ggagataaca ttggtataaa tgttgtcaat ccaatcagta
     5941 aggcaacact aattaaagtc ctcttcttcc caatttttgt agaaatcacc ccaaagataa
     6001 acgtacctgg aattgtacca aaagacataa ctgcaaatat ttcttctgtt tgtcttaatg
     6061 aaaaaccaaa tcccccatca gctaaagcat aaatcattgt tggtttaata aaagtaccta
     6121 ccataaaatt gtagcaataa aaaaagtata ttaaagcaat tgaaaaaaat gcaattcctc
     6181 tttttaagtt tgaatagtct tttcctaata tgatatcatt ttctatttca accgttgttt
     6241 tcatattttt ataattcccc cttttaaata ttagtcataa ttttgctaaa agtttaaaca
     6301 tctatttttc atatacattc cctacttaaa ttttacaaaa caatacttcc ctatatacat
     6361 ttgaaaacta tttatactct aaacagcctt attcttctac tcccatatac atttgtttta
     6421 agacatccat accatcaaaa gttatccact cctcctttac tttaccttgt agtatatgat
     6481 aatgattgat tcccattacc tctatatgct ttccagaagg ctgtccaaaa aatcctatac
     6541 cttcatttat tccacgtaat ctccatctta cagatacatc ataaccttca tttttttctc
     6601 tttgattaca agttactctt tccacttcat aacttccatt tggaattgat gcaagtaagc
     6661 ttacaatcat tccttgaatc tcatcataac catttaaatc tttatcacat ataaaatgga
     6721 ctactgcatt atcatgatag tattttttaa cttcatttat atatttataa ttataaatac
     6781 gacttgtcat ttcaagcatc atttcgccaa cagaatcatc ttttgcttga tacttagttg
     6841 gcataaattg accatccata gattcacaga taccataagt tgattgtaaa ctaagcactt
     6901 tagattcact tgcctttgcc atactctttg ctacttcctg agggtttaat cctaattgag
     6961 taacaatcca taaattatct ctaaccaacc attcttcgtg gattctattg ttagttgtgg
     7021 cacaatcaat tacagttcta aagtttattt ttctacctgt tgctggtcca aaattactat
     7081 cacctaagtt agttgctgta gacaaaacac ggtgagaaga taaaaaacca tcagcaccaa
     7141 agttagacca tattacattc tgaccaatta atcttctatc aggaaacgca tgcaatgttt
     7201 gaagagtgtt ggaaataaca tctttaatac caactaaatt tgaagaacca caatgcattg
     7261 taacattatt gtgatatgta tcataaataa cacctatacc tttttcttcc cagatattat
     7321 gtgtaatttt cataatataa tctacaaaat ctctatactt agaatcaaat cctggtatct
     7381 cctgctccct ttttgtctgc tttgaaaaat cattgtaatc cattactcca acaggcgtta
     7441 cttctaatga atctccatag taaatttttg tagatgacat attatcttcc cccttaaata
     7501 taactttttt atttttttca gtaagagtat ttgtatttaa tttatctgta tccattgtat
     7561 ctatgttaac tttatttatg tcggatttat ttgtgttggt tttatctata ttccctttat
     7621 ctatatctcc tttatatgta ttcgttttat tctcatttgt ttgattttca tttatattac
     7681 taaaattagt ttgatttgtt ttagctttgt tttctttaac cgtacatgat tcaagcccac
     7741 ttgtagaagc ttctttttct tcccctttac ttgttacatt atttttgttg gtagatgcca
     7801 ctttatatcc cccttcgttt tagaatacgt atgcataatt aagcattaaa aatactttta
     7861 gttctctcaa gaatatcaag cccttgttgt ttcaaccaat aaggaatatc aattaacacc
     7921 cagttttctt gaagtttatc tcctttacga taataaacat caacaacttg catatctgct
     7981 ttaacctctc cacctggtaa tcctaaaaaa cctccacatg gtacattagt cagattaggc
     8041 catccaaaga aacaagcaaa attaccttct gcaaatctac atacatgccc attaaaagtc
     8101 tttcctttta aattatttct aaatggtagc tgatgttgtt cttgatatct aggtatagta
     8161 taagaagcac caattccacc tggaccatac caaatcatat ctttagccca agatttttca
     8221 agtatttctg gtggacatcc catagcccca ctttcattta attcagataa atcatctacc
     8281 attttattta ctaatgctaa agtttttact ccttcaactt catctgcatc ttcaaaaagt
     8341 aatccattat ggtcaattgg gcctggatat acaaaatatg aacctgtttg tggtggcaat
     8401 ggattaactc ctgcttgaat cataaacccg attatatcta caaataatcc agttttagca
     8461 attttaccat cttgaacaca gttaaattct gcatacctta aactaatcat tttacgagta
     8521 tgtttaatac caagccaatc cttctgaacc tcccatgact aaagtcacag ggttcctgct
     8581 tcatagaatt ttgcatacta aaatatgtct tactaattct ccacaggcta tccccgtagt
     8641 tcctacggtt cttacataag ttatttattt tgaattgtta ttaatcttaa tccttctttt
     8701 aatatattta tacttgcatt tatatctcta ttgtgagttt cattacaact aggacaaatc
     8761 cattctctta tatttaagtt cttcacttcc tcgtttttgt acccacattt attgcatatt
     8821 tgtgaacttg caaagaattt acccaccttt acaatttgtc taccatgcca attagccttg
     8881 tattctaatt gacgaataaa ctctgaccat gacacatcag aaattaagcg agatagttta
     8941 cgatttctaa tcatattctt tacttgtaaa tcttctatgc aaataatatc attctctttt
     9001 attaatttag tagatagttt ttgtagaaaa tcctttcttt gattagctat atgttcttga
     9061 agtcttgcaa cttttaatct tgctttattt ctatttaa
//
LOCUS       DAEMAE010000146         9096 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEMAE010000146 DAEMAE010000000
VERSION     DAEMAE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.148"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..1290
                     /locus_tag="KPH93_002664"
     CDS             85..1290
                     /locus_tag="KPH93_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG1689469.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1361..1582)
                     /locus_tag="KPH93_002665"
     CDS             complement(1361..1582)
                     /locus_tag="KPH93_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689470.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1724..2323)
                     /gene="recR"
                     /locus_tag="KPH93_002666"
     CDS             complement(1724..2323)
                     /gene="recR"
                     /locus_tag="KPH93_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG1689471.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2336..2686)
                     /locus_tag="KPH93_002667"
     CDS             complement(2336..2686)
                     /locus_tag="KPH93_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG1689472.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2745..4382)
                     /gene="dnaX"
                     /locus_tag="KPH93_002668"
     CDS             complement(2745..4382)
                     /gene="dnaX"
                     /locus_tag="KPH93_002668"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG1689473.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKKYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4459..4723)
                     /gene="ffs"
                     /locus_tag="KPH93_002669"
     ncRNA           complement(4459..4723)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPH93_002669"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4761..4852)
                     /locus_tag="KPH93_002670"
     tRNA            complement(4761..4852)
                     /locus_tag="KPH93_002670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4816..4818),aa:Ser,seq:gga)
     gene            complement(4853..5308)
                     /locus_tag="KPH93_002671"
     CDS             complement(4853..5308)
                     /locus_tag="KPH93_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG1689474.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5629..6900)
                     /gene="serS"
                     /locus_tag="KPH93_002672"
     CDS             complement(5629..6900)
                     /gene="serS"
                     /locus_tag="KPH93_002672"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG1689475.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIR"
     misc_binding    complement(7026..7231)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7359..8240)
                     /locus_tag="KPH93_002673"
     CDS             complement(7359..8240)
                     /locus_tag="KPH93_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG1689476.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8252..8761)
                     /locus_tag="KPH93_002674"
     CDS             complement(8252..8761)
                     /locus_tag="KPH93_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG1689477.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGILAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(8889..9005)
                     /gene="rrf"
                     /locus_tag="KPH93_002675"
     rRNA            complement(8889..9005)
                     /gene="rrf"
                     /locus_tag="KPH93_002675"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2918 a         1605 c          983 g         3590 t
ORIGIN      
        1 ataaccttat tcacaaagac agtttttata tatcaaaata taatacaata taaatactat
       61 gctacatttt aggaggattt tattatgagc aaaaaagtat taataatgtc tgcttccact
      121 ggtggtgggc ataatagggc tgctctagct attaaagaag aacttacttc caaaacactt
      181 gatggagagc caattgaatg tgaaataata gatagtttga aattagttaa taatacaatg
      241 gataaaataa tttcaagagg atacgaaaaa tctgctatat atactcccaa agcctatgga
      301 agtgtatata gattatctga aacaaatctt ttatcaaaaa atgaatttaa agataatcta
      361 ctaataacat tcatggctaa aaaatttaaa aagcttattc gaagtgaaaa accagatttg
      421 attataggaa cgcatccttt tccaatgatt gctctaagta cattgaaaaa aaactttaat
      481 ttacataata atgaatcaaa tgcttacaca gaacattttt ataaacatta tacaaatacc
      541 attaatgttc ctcctcttat ttctgtatta actgattata ctactcattc tacatggata
      601 caaaatgaaa ttgattacta tatcgttggt catgaatatg taaaagaatt attggtgttt
      661 gatggagttg aaccgagtaa aattagaaca tttggaatcc ctgttgaaaa atcttttctc
      721 tctcacagag ataaagatat tgttctttct gagttaaatt tatctcctga taaattaact
      781 gttttactta tggggggtag ctttggggct ggaaatataa aagagacact tgatgaatta
      841 ctggatactg atagagactt tcaaatactt gtaattactg gtaagaatga aagcttaaaa
      901 gaaaaaatcg aaaaaaaact tatgtctcgt tatcatgata aaaatgtatg tgttcttgga
      961 tatactgata agatgaatga tattttagct tctatagatg tattagtaag caaacctggt
     1021 ggacttacta caactgaggc tcttttaaaa gatgtaccta tgatagttcc ttattatata
     1081 ccaggtcaag aagaagagaa tcttgacttc ttatctaatt gtggtgcagc acttagaact
     1141 acaaaaaaat ataacttgtc aatattacta aaagttttaa tagatgaccc tagcagatta
     1201 gaaatgctaa aaagaaatat taaatcaata agaaagtcaa attcagccca aaatatagct
     1261 aatttaattt taaatataat ttctggttga taaaataaat aaaatcccta tagtatatat
     1321 attatctatt agggattttt tattttctaa ttatttaata ttaaattggt tcataaaagt
     1381 cttcttcgta atcatactga taatttttat aattatttct aaattcagca tttttatttc
     1441 gtacattttt tttagttttt ttcttattag aaaaacttat atcctcaaaa tcatcatatt
     1501 taggtttatc ttttgatttc atttttttaa ctcttttttc tttcattgca ttatgttctt
     1561 ctaagttcat aattggtttc atgttgcacc cctatcaaaa tttttaaatg atttatacaa
     1621 caaattatac aatacagaag aaatttattc aataaatcta aatcaaaatt aatttgaatt
     1681 taacccaaaa aaattacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc
     1741 cttctagtgc ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa
     1801 gaccatgagc tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca
     1861 tggctgtagc ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct
     1921 gattgccaag tctttggata agttccttta tttttaacat atctggtcca attccatcca
     1981 ttggagaaat tactccattt aatacatgat actggccttt aaactctctc gttctctcca
     2041 tagctgctac atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat
     2101 ttgaacacat gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta
     2161 tttctctttt tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat
     2221 taataatatg aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa
     2281 attcctctat aagtctcgtt attggacctg tataaacttg cataaatacc acctactaga
     2341 ataacccagg tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat
     2401 caatatttct caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat
     2461 catctggatc aactacttct ggtttgatag ttatatctat aacttctttt ttaccattaa
     2521 cttttacagt aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt
     2581 gttgagcttt ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat
     2641 ttccaccacc aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc
     2701 ctaaacttat tttcatttta ttattatatc ataatagtat tttattattt actctcattt
     2761 tcatcaatac tatcttttat ttctaatatc tcttcactca ctatgttctt taatatctct
     2821 tcaccaatat cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt
     2881 actattttta cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt
     2941 attgtatctt gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca
     3001 tacaacatat tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc
     3061 atttttttat cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata
     3121 acgtcctcat tttttacatc ttcataaact acattttctt gttttacatc tatttctcta
     3181 tcgacctcat actttttttc tttattattc ccaatttttt cagacttaaa gttccccagt
     3241 tcaatcattt tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac
     3301 attggttgag caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac
     3361 tttatatcat cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct
     3421 atatttttag actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca
     3481 tccaattctg atgaaacctt acataccatt aaatttctaa aatgatcaat taaatcattt
     3541 ataagatttc ttatatcttt accccataag acaaattcat ttaatatctc aagagacttt
     3601 ttagtgtcct gctctacaat acattgagac aattcaaata attgttctat atttacgctt
     3661 cccattaatt caacaacatc tttataatct attttacttt ctccaaaaga catacattgg
     3721 tctaatatac taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga
     3781 gctttatcat ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata
     3841 tctttaactg taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt
     3901 attttgtgag gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag
     3961 gtctttaata atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat
     4021 actttatatt tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca
     4081 tctacactgt tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat
     4141 acagattcac atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt
     4201 actgctcttg aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac
     4261 aaataagcat gtcctatatt atcactatat atttggtttt ttaaagtctt aataatatga
     4321 tcttgaccaa taacatcttc aaacttttgt ggtctatatg ctctatataa tgctttatgc
     4381 atctttttac ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag
     4441 tcttttatat attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa
     4501 acagttagct caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc
     4561 cttccggatc tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac
     4621 ttatttaggg cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg
     4681 gattgcaggt tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat
     4741 ttaattactt tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta
     4801 cccatatact cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc
     4861 tttttctcaa tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat
     4921 caaaagctac ttttacatta ttattattaa aataatctaa tttaaactca tgttgttttt
     4981 ctatatatga attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa
     5041 ctattgcacc actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca
     5101 acctccaacc acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgctg
     5161 tagaatcttt taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa
     5221 ctataatagc acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg
     5281 cttctttcat ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct
     5341 tatcataatt cattttataa ttcaacttaa ttttattttg tttatttaat tattactata
     5401 tatagctata ttttttttaa attagttcta aaataatttt tagatattca tacaagagta
     5461 ttttataaaa atatttagtg ttttatgatg aaaattctgc catgtaaatt aacataaaaa
     5521 aactatgaaa ttaatattta taataatttc atagtttttt aatttattgt ttactcataa
     5581 tatattctct tcattaaagc atactctaca tatacaataa atataaaatt atcttataac
     5641 atcaacaccc atataaggtc ttaattcttc tggaactact acagaaccat ctgcttgctg
     5701 ataattttct aatatagcag ccaaacatct tcctatcgct aatccagaac catttaatgt
     5761 atgaacatat tctgcttttg attttttatc tcttttaaat cttattcctg ctcttcttgc
     5821 ttggaaatct tcaaagtttg aacaagaaga tatttctaca tatctattgt aacttggcat
     5881 ccaaacttct aaatcgtatt taaatgcagc tgtaaatccc aaatctcctg tacatatttt
     5941 tacaactcta tatggaagac ctaacatttg caacatagtt tcagcatcat gagttaatga
     6001 ttctaattca ttataagaat cttctggctt aacaaattta actaattcaa ctttattaaa
     6061 ttgatgttgt ctaactaaac ctcttgtatc tcttcctgct gaacctgctt cagatctaaa
     6121 acaaggagta tatgcacagt atttaattgg aagctctgca acatctaata tttcattagc
     6181 atgtatatta gtaacaggaa cttcagctgt tggtattaag aaatactcta atccttctat
     6241 tttaaacata tcttcttcaa atttaggaag ttgaccagta cctataaaac tatttctatt
     6301 ggccataaat ggaggtaaga cttccgtata gccatgctta gctgtatgag tatttaaaaa
     6361 gaaattcatt aaagatcttt ctaatctagc tcctaatccc ctatataaag taaaccttga
     6421 cccagttatt ttccctgctc tttcaaaatc taaaatacct agacctgtac ctatatccca
     6481 atgcgcttta tgttcaaaat caaatttagt aggctctccc caagttctta tttgaacatt
     6541 atcttcatct gtttcacctt gtggtacctc tggatgagga acatttggta ttctcattaa
     6601 tgtatattcc atttttgctt ctacttcttt tactttctca tcaatacctt ttattttatc
     6661 tgataactct ttcaatcttg ccttttcttc tgttacatct tttccttctt ttattaattg
     6721 aggtattttt ttagaattta catttaactc atttttcata acttcaactt cttgaagtat
     6781 ttctcttctt ttattatcca attccactac agcatcaagg tcaaattctc tttcacctct
     6841 tctttccata gcttttttga tatcatctag attttctctt attcttttaa tatctaacat
     6901 attaattcct cctaataatt ttgataatat aagttataat tccaacttaa tttacaaaca
     6961 ttatacttgt aaactcttag tttaaacaaa ttttaaaaat aaaaaaagac ctccgtttac
     7021 cctataaaag ggacgaaaat cttttatccg cgttgccacc ctaattgact atactaaaat
     7081 gtataatcct ctcagaccta taacgtaggt aaacgtctag ctttaactag aagctctaga
     7141 gtagattcaa aaaccattgt aatgagtttt caccatccac tcattctctg aatacaatct
     7201 gttttttact attttccttc acagcttttt aactaattgt tttatttgat aatcattata
     7261 ttatatatat taacatcttt caatactatt attcaaattt ttttagaatt ttctttaatt
     7321 ttttatatta aattcatttt ttctatttaa ttaagacctt atcttttatc cattgtattt
     7381 aaattacctg tattattaaa taataatgaa gaaggaacaa atggaacttt tatatcattc
     7441 tcatcaaatt tattttttac cttttccatt acatcaaagt atacatccca gtaatcatca
     7501 gtttttaccc aaggtctagc gactatttgc atggtgttat ttcctataga gtttagacct
     7561 attataggct ttggatcatt taatattctt ttttcgtttg taaaaatttc atttaatata
     7621 gacttaacta catctatatc actatcatat gaaatattat atgaaaaatc tattcttctt
     7681 ttgttctgat gtgtgtaatt aattatatta ttacttgtca gttgaaaatt aggtataatt
     7741 atagttttat tatctggagt cttaagaaca gtgctaaata tttgtatgtc agaaactgta
     7801 ccttctacac ctgaaccttg aatataatca cctatattaa atggcttaaa aaataaaata
     7861 atcataccag aacctaaatt tgataatatt tctttaaaag ctaaacctat actaaatcca
     7921 gcagctccta acacagccac aactgatgta gtttgtatcc ctataatgcc taaacttatc
     7981 aatgatataa tagataacat agatatatat attccatata caataaaatt atttacacct
     8041 ttactcattc catatttgat tagaaacttt gaaaccatcc ttcttactat tttagatata
     8101 taaataccta tcacaaatac aattattgca tatataatat ttggtatatt atcactaaaa
     8161 ttatcgagca tattttctaa agttctctta gttatattgc tatttgttga ggcagtctta
     8221 gccaatgata aaaaattcat ttaactcctc cttatgataa tattccgttg tcctttaact
     8281 cttttactat agcttctata gtttcttctt ttattgcctc aattgtatga atatggctac
     8341 attctgtaag aaccgataat tgtttaaatt catttgtctc aactttcttc ataaagttgt
     8401 ctatatccat tttagaataa atattaagtt ctgcagttat ctgaccatat gttggatgat
     8461 ttacaatgac atcttttata ataccaccta aatctacaat tatctttaat tcatcataaa
     8521 tttctttaga atccttatga tttttacatt ttatttgttt tgtaaaatac ggttgattat
     8581 atattatata tccttgaggt gtagctatta tttcaaatcc tctagctctt ataagggcta
     8641 tatcttgaac tattacttgc ctacttacat ttaatagttc agctagtatt ccacctttca
     8701 caggatgctt agattctttt aaaatatcta ttaatttgct tcttctttca ttagaattca
     8761 tattacacct tctttctaaa taaaaaactt tacctatact atataatttt atattttatc
     8821 ttattataaa tcaatattga ctttatattc tatttaatct taacttttat aataaataaa
     8881 aaaagactac gtggctacgt cctactctcc caggcagttt cccaccaagt accatcagcg
     8941 ctagagagct taacttctgt gttcggaatg ggaacaggtg tatcctctct gctattgtaa
     9001 ccacataatc tttatgctta atatttctat taagttttta ataagtttat agaacctagt
     9061 aatactagtt tctttagagt taacactcta aaaact
//
LOCUS       DAEMAE010000147         9050 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEMAE010000147 DAEMAE010000000
VERSION     DAEMAE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.149"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            859..2529
                     /locus_tag="KPH93_002676"
     CDS             859..2529
                     /locus_tag="KPH93_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG1689478.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLDGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTILLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDVDNFKNINEKYGHSYGDKVLRAIATEVLEYIPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIHMYTNTQHEIESNKYYCTITAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2647..3381)
                     /gene="pflA"
                     /locus_tag="KPH93_002677"
     CDS             complement(2647..3381)
                     /gene="pflA"
                     /locus_tag="KPH93_002677"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG1689479.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMGKALKLARHLESRNIP
                     VWIRHVLVPGITDDVDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3541..5772)
                     /gene="pflB"
                     /locus_tag="KPH93_002678"
     CDS             complement(3541..5772)
                     /gene="pflB"
                     /locus_tag="KPH93_002678"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG1689480.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDNIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6064..7125
                     /locus_tag="KPH93_002679"
     CDS             6064..7125
                     /locus_tag="KPH93_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG1689481.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVILFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNAIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7216..8829
                     /locus_tag="KPH93_002680"
     CDS             7216..8829
                     /locus_tag="KPH93_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1689482.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
BASE COUNT         3398 a         1184 c         1385 g         3083 t
ORIGIN      
        1 aaccctttac aacaaatgga aactatggat gtaataatta aaataaaaaa tattatttta
       61 tatttataaa cattgacatt ctaattttgt agtgtaatcg tattttttat taattcttac
      121 aaaatcaatg aaaataagga tatccatttt aaagtacata aatttgaaaa atccagagaa
      181 atatacttaa attatgtaag taaaagaggg gttgaatcag ttgaaaatgt aatactaata
      241 aataatacat caatcctaga taccctgtaa gcaactctat tttcatgtac taaaaatgta
      301 cctattttat taacttgaaa agaataaatt aaatgtaagt acataagaag agataaaaaa
      361 caaaaaatat ttatataaat aaatataaaa aataatataa tccctattaa aaaatttata
      421 gataatgaag aaatatcaaa atcttatatt ataggaagag gcacttcttt accaaaatct
      481 atagaaaaac aataccaaat ccaaagacaa taggtggaga agataaaaat aaaataaatt
      541 caagagtaat agaagagagt tttatatgat aaagaactga gaactcttta tataacgaaa
      601 aatagaattt taatagaaaa tgatttaata gatgatttaa catggtgttt tagcaggtaa
      661 aaataaatca ccagtaatga ttatagaaaa attactttac gaatctcaaa aaacttagtt
      721 aagagtaagg gatttgataa gataactaaa gttgggaaat gtgtttgatg agataaaaaa
      781 attagtaaaa tagtataatc taaataagaa tattttgttg attaaactac actaatagta
      841 atattgtagg gggaaaagat gagagaagaa tcgaactctt taagtttaga ccatatagaa
      901 attttctttg aacttttatc tcaaagtaca gaagattata tatttttttg ggatataaat
      961 aggaataaat ttaaaatttc ttcagcaatt tttgatgaat ttaacttaag taaggagata
     1021 gaatctgatt tagtcaattg ttggagtaaa attgtttatc cagatgatgt tcaaatatgg
     1081 aaagatgata tacaagaatt actagatgga aaaaaaggtg agcacaattt agaatatagg
     1141 ttgattaata aatatgaaga aatagtatgg atttcttgta gaggtaaagt ttatgtgtca
     1201 gatgacccaa aaactatact tctagtcggg agaataaaga atattggtga aaaaaacaaa
     1261 tttgattcta tcacaggaac gtggaataga gagcaatttg aacatagaat gaattatctt
     1321 ataaaagaga aaatttataa aaatggtgct atgtttatca tggatgttga taattttaaa
     1381 aatataaatg aaaaatatgg acattcttat ggtgacaaag tattgcgtgc aattgctact
     1441 gaagtattgg aatatatccc aaaagatgta agattgtata ggttagatgg agatgaattt
     1501 gcattttttt accctatgtg tactaaagaa actatagaga aaatatatga aaagatacat
     1561 atgtatacaa atactcagca tgaaattgaa tcaaataagt actattgcac aataactgca
     1621 ggagttgcta tgtatccaga agatggagat aattatttag atttatttaa acatgctgat
     1681 attgcattgg atatagcaaa aattagtggt aaaaatagaa ttaagttttt ttcacaagag
     1741 ctctatgaaa ataagctaaa ggtaatttca atgcaacaaa aactaagaga gtgcgttgaa
     1801 aataatttta atgactttga gctgtttttt caaccccagg taaatgctgt tacaaaagag
     1861 gtaatagggg cagaggtact tttaagatgg cattcgagta cttatggaga agtatctcct
     1921 gttgaattta taccaatact agaacagagt aatttaatta taccagttgg gaagtggatt
     1981 ataaaagaag ctgtaaaaca gtgtaaagaa tggcataaaa taaatccaga ctttaaaata
     2041 tctgtaaatg tatcgtatat tcagttaaag gaagattttt ttagagattt tatagtagaa
     2101 tgtttagtag agtatcaatt aagacctgaa tttttaattt tggaattaac agagaattgt
     2161 tggataccag atataaattt attaaatgat aaatttatta gtttaaaggg tataggagta
     2221 tatattgcaa tagatgactt tggtacagga tattcatctt taaattacct taaagaactg
     2281 tctgttaata taataaaaat tgaaagaagt tttgttaaga atattacata caatagttac
     2341 gaatatactt ttcttgaata tattattaaa ttagctcata ttataaattt aaaagtttgt
     2401 gttgagggaa tagagtctta tgaagagtat gatatagtaa aaagcttagg tgtagatatt
     2461 attcagggtt ttttatttgg aagacctgta agtgcctctg aattttataa atttcaacta
     2521 tcaaagtgat gtatataaaa tgagatatat agtatataaa aatagggtaa agtggaagtt
     2581 ttgaagtcta ttaaaaagtt acactttacc ctattttgtt atgtcttcta taaagattaa
     2641 tatatactaa ctattatata ctttcactcc aaatttagag attatttcac tagctttttg
     2701 gacatcttcc ttagtggcat caggaacatc ttttagttca taatcgatac ctaaactttc
     2761 ccatttgtgt attcccatag aatggtatgg tagcaattca aatctttcta catttttcaa
     2821 tgtagctaca aattgaccta gtttttctaa attatctaca tcatcagtaa tgccaggaac
     2881 taaaacatgt cttatccata caggaatatt tcttgattct aagtgtctag caagttttaa
     2941 tgccttaccc ataccaacac cagtcaaatc aatagatttt tcctcaacca tatgttttaa
     3001 atcaagtaaa accaaatcag tattatctaa aactgggtct atagtttcta tatctacgaa
     3061 tccagatgta tccaagcaag tgtgtatatc attttctttt gcttttttaa ataattcact
     3121 taaaaattct ggttgaagag tggattcacc accagaggca gttatacctc caccagaaaa
     3181 cttcataaaa gaagtatact taagagcatc cgttattatt tcatcaaccg tgtattcttt
     3241 tcctgattta atatcccatg tatccctgtt atgacagtat ttgcatctaa gaggacaccc
     3301 ctgaaaaaat aatatatatc ttattcctgg tccatctaca gtaccaaatg tttctataga
     3361 atgtattttt cccttaatca tagagttcac ctcacaatat attttaataa tcttatcaat
     3421 agttataata tttctctaaa aaaggccatc ttataccaga aataattcaa atataagata
     3481 gccttttatt tttatttttt acacagtagt aatcttagtt aaagtataaa aatttaaaga
     3541 ctaagccatt ttaccgtgga atgttctgtt tataacatct aattgttgct ctttagttaa
     3601 ttttatgaag ttaactgcat atccagatac tcttatagtt aattgtggat attcttctgg
     3661 atgttccata gcatcttcta aagtagctct atcaaataca tttacattta agtgatgagc
     3721 attttgagcg aagtatccat ccatcattgc agaaagattg tttattcttt cagtcatatc
     3781 ttttcctaaa gcagcaggta ctatagagaa agtattagat ataccatctt gagcatgctc
     3841 atatggtaat ttagcaactg aagataatga agctaaagca ccattagtat ctctaccatg
     3901 cattgggtta gcaccaggag caaatggagc accagctctt ctaccatctg gtgtattacc
     3961 agttttctta ccataaacaa catttgaagt tattgtaagt atagattgag ttggataaga
     4021 atttctataa gttttattct ttcttatttt attcataaag ctttcaacta actctacagc
     4081 tatattatca actctatcat cattgtttcc gtattttgga taatctcctt ctatttcaaa
     4141 atcaacagct attccttgtt catttctaat agttttaacc ttagcatgtt ttatagcaga
     4201 taatgagtct gcacaaactg ataacccagc cataccacaa gccatagttc taaatatatc
     4261 tctatcatgt aatgccattt ctaatgcttc ataagaatat ttatcatgca tataatgtat
     4321 tacatttaaa gtatttacat ataatgttgc taaccaatca gtgaatggtt caaatttact
     4381 cataacttca tcataatcta ggtactcaga agttataggc tcaaatcttg gtccaacttg
     4441 aacaccagat ttctcatcta caccaccatt tatagcgtat aataaagttt tagctaagtt
     4501 aactctagct ccgaagaact gcatttgttt accaattctc attgcagata cacaacaagc
     4561 tataccatag tcatctcccc agtatgctct cattaaatca tcattttcat actgtactga
     4621 acttgtatct atagatactt tagaacagaa atctttgaat ccttgaggta atttagttga
     4681 ccatagaact gttaagtttg gttctggaga aggtcctaat gtatatagag tatttaacat
     4741 tctaaatgag tttttagtaa ctaatgttct accatctatt cccatacctc ctatacactc
     4801 agttacccaa gttgggtctc cagagaatag gttattgtaa tcaggagttc ttaggaactt
     4861 aaccattctt aatttcataa caaaatggtc catatactct tgtaattctt cttctgttac
     4921 gattccagct tttaaatctc tttcaaagta tatatctaag aaagtagatg ttctacctaa
     4981 agacattgca gcaccatttt ggtctttaac agcacctaaa taaccaaagt ataaccattg
     5041 tacagcttct tttgagttag ttgctggttt tgatatatca aatccataag attcagccat
     5101 ttttttaagt tcatttaaag ctgatatttg ctcagttatt tcttctctta atctgataac
     5161 ttcctcatcc atgcaagaaa cttctaatga tttcttttgc tcatttttat cttctattaa
     5221 agcatcaaca ccgtatagag caactcttct gtagtcacca attattctac ctctaccata
     5281 agcatctgga agacctgtta ttataccaga ttttctagca gctctcattt ctggagtata
     5341 aacatcaaaa actccttggt tgtgtgtttt tctgtattta gtaaatatat cttttatttc
     5401 tgggtctaat tcatatccaa aagcttcaca tgaattttca accatcttga taccaccata
     5461 tggcatgaca gctcttttta aaggggcatc cgtttgtaac ccaactattg tttctaattc
     5521 tttatctaaa tatcctggag catatgcagc tattccagaa acagtttttg tatcaacatc
     5581 taaagtacca ccattttctc tttctttttt aaataggacc attgcttcgt cccataattt
     5641 tttagtattt tcagtagcac cagctaagaa agagtcattt ccctcatatg gagaatagtt
     5701 taattgtata aactctctaa catttatttc tttagtccat cttcctgttt taaatccttg
     5761 ccatgcattc attaagtatt tcctccttag atttaaatga taaataatgc tattaaaatt
     5821 aatttttata tacttaattt attttatata tttttttaaa aatatatttt ctaatataat
     5881 aatagcatat tattcgtatc ctgtatacat aaaaataaaa aattgtcgct ttaaagtata
     5941 aaacagatta tatgttaata atgtggtgaa attataccca tatataaata aaataaaaca
     6001 aataaatagc taattaagga gaaaatattt tttatttttt gtttaggata aataaatttt
     6061 attatggaaa aaagaaaaag catacatctt cacaaactga atatttattt aataggggga
     6121 ggtgatagat tgtttatcac agtgatatta tttttagtag gtttcctatt aattactaaa
     6181 ggcgcagata tctttataaa ttgtactgta gagataggta gaaaaacgaa tatatcagaa
     6241 attatacttg gagctactat agttagtttt gcaacaacat tacctgaatt tacagtgtct
     6301 ctacttgctt ctattgatgg tcatactact atgagtttag gaaatgcagt tggttctata
     6361 atatgtaata caggattagc attagggttg gttgttttta taagtccatt taatgtagat
     6421 aaaaaaatgt ttttttctaa gtctttacta ttaatagtat ccgttatagt tttaatttta
     6481 ttgagtttag atggagttat aacaagaggt gattcattat tacttataat aatattaatc
     6541 ttttatatgg ttaataatta tagaagtgtc gttggaaaat ctgatactaa aaatagaaat
     6601 attaaaaata acgctattaa agacatatct actaaaaaag ggaaaaatta taggggtttt
     6661 tccgtattag aaattgtaaa aatactatta ctttttgcta caggattgat aatgatgata
     6721 atcggttcac aaatccttat agagagtgga gtaataatag ctagtttttt aaatatacct
     6781 caaggtatag taagtttaac tataatagca cttggaactt ctctgccaga gatagtttcg
     6841 tcgattactg ctataagaaa aaatcatcat gaaatatcag taggaaatat tttgggtgca
     6901 aacatattga atatagtatc agtaatagct gtatcagcaa taccaaataa cataccaata
     6961 ctatctcaaa atagacaatt ggatatacca ttcatgatat tattattatt aatagtaatc
     7021 ataccaacac ttaaatctaa tagacttagt agaatacaag gtatattgat gttattcaca
     7081 tattttctgt acatatcaat tttgtatttc atgtatatta tttaactata ctgtttaaaa
     7141 tattaattgc agaatatcct aaaaaagtat ataatggata ggttaagatt aattgataac
     7201 ggagaggaat aatatatgaa tataacaaag tttgaagatt taccaataag tgagggaata
     7261 aaaaaagcta tagctgaaat ggggtttgaa gaaccatctc ctatacaagc acagtctata
     7321 cctgcgattt tgtcaggtaa agacgttata ggacaggctc aaactggtac gggaaaaaca
     7381 gctgctttta gtatccctat actagaaaca atagacccaa ataatagaag tttacaagct
     7441 gtagtgcttt gtccaacaag agaactggca atacaagtat caacagaaat aagaaagtta
     7501 gctaagtatt cgcacggaat aaagacatta cctatatatg gtggtcagcc aatagataga
     7561 caaataaaat ctctaaagag tggtgtgcaa gttgtaatag gtacaccagg acgtacaata
     7621 gaccatatca atcgtaaaac attaaagatg gataatgtaa aaatgattat attagatgaa
     7681 gcagatgaaa tgttagatat gggatttaga gaagatatag aaatgatttt aagtaagatt
     7741 ccagaagaaa gacaaacaac tttcttttca gctactatgc caagaggaat tttggaatta
     7801 actaagagat atcaaaaaga cccagagcat ataaaagttg ttagaaaaga acttacagtt
     7861 tcaaacacaa agcaatacta tatagaaact aggtcatcaa ataaattaga agtattatgt
     7921 agattagtag atgtatatga ccctaaacta tctgttgttt tctgtaatac taagagaaaa
     7981 gcagacgaat tggtaggaga tttacaagca agaggatatt ttgcagatgc attacatggt
     8041 gacttaaaac agactcaaag agatatagta atggataagt ttagaaatgg tacaatagat
     8101 atacttgttg ctactgatgt tgcagctaga ggtatagatg ttgatgatgt tgaatgtgta
     8161 ttcaactatg atttaccaca agatgaagag tattacgttc atagaattgg tagaactggt
     8221 cgtgctggta gagaaggtat gtcatttact tttgtatttg gaaaagaaat gagaaagatg
     8281 aaagatatag aaagatatac taaatctaaa ctgataaaac ataatatacc aacaataact
     8341 gatgttgaag agaaaaaagt aggtactttc tttgcacaag ttaaacaaac tatagaagaa
     8401 ggtcatttaa ctaaacaatt acaatggtta gaaggcttct gcaatgatga agattatgca
     8461 atggttgata tagctgctgc acttgtaaaa ctatctctag gtgaagaaat gaaagaagag
     8521 ataatagaag aaaaacctag aagagagcgt ggagatagaa aaggtggtac tggagctaaa
     8581 gatggtatga ttagattatt tatcaatatt ggtagaaatc aaagagtaca agcaaaagat
     8641 atagttggtg ctatagctgg agaagttgga ataccaggta aagtagtagg tactatagat
     8701 atatatgata agtatacttt tgttgaaata cctaaaaaag atgcaaaaac agtaattgaa
     8761 aagatgaaag atattaagat aaaaggtaat aagattaata tagaaaaagc aaataagaag
     8821 aaaaaataat ttatagtata taaaactata attagaaaat atataaaata atatattgga
     8881 tatttaaaaa gcctaatgtg ttttcaaaac agatgtgtag aatctgtaat agttaacaac
     8941 ataaggcttt tattgttttt atataagaaa tctgaataac ttactgttaa atctttaaac
     9001 tactccacct tacttatgtt aaggatggag tattctactt atttttgata
//
LOCUS       DAEMAE010000148         9037 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEMAE010000148 DAEMAE010000000
VERSION     DAEMAE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.150"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..204)
                     /locus_tag="KPH93_002681"
     CDS             complement(<1..204)
                     /locus_tag="KPH93_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689483.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGK"
     gene            complement(705..1685)
                     /locus_tag="KPH93_002682"
     CDS             complement(705..1685)
                     /locus_tag="KPH93_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689484.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1849..2721)
                     /locus_tag="KPH93_002683"
     CDS             complement(1849..2721)
                     /locus_tag="KPH93_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1689485.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTILNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     ILAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(2895..3446)
                     /locus_tag="KPH93_002684"
     CDS             complement(2895..3446)
                     /locus_tag="KPH93_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689486.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHVYKNLNKGKTIFHNIEVYR"
     gene            complement(3466..4368)
                     /locus_tag="KPH93_002685"
     CDS             complement(3466..4368)
                     /locus_tag="KPH93_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG1689487.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLYDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRSTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4383..6755)
                     /locus_tag="KPH93_002686"
     CDS             complement(4383..6755)
                     /locus_tag="KPH93_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG1689488.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSSLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDAKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(6976..7665)
                     /locus_tag="KPH93_002687"
     CDS             complement(6976..7665)
                     /locus_tag="KPH93_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689489.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKG
                     SLKEQVEDKKEESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFMVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SIVYKFDISNH"
     gene            complement(7906..>9037)
                     /locus_tag="KPH93_002688"
     CDS             complement(7906..>9037)
                     /locus_tag="KPH93_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1689490.1"
                     /translation="FYKIGYIKYSVFFYLFSVYYKCLDAVIYRILFFAITGNVISSKE
                     SLLVPRLESIKIQIYSNLIIILIYLIFSFFRKSIKLISQDKRNYVYLLFALLANTMNM
                     FVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELI
                     KNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEIDEYVNNLKDEIKT
                     FKYIYNTGNMILDIIINEKSEICLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNA
                     IEACDKIVDENINKYIRIKGTITKSFFVLKCENSKLNQLTFNKNILLTNKKDKFVHGI
                     GIQSIKSSLQKYNGELLFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         2941 a         1589 c          847 g         3660 t
ORIGIN      
        1 ttttccacaa caatactcat atgtctccac attatttgga atatttacaa atacaaattc
       61 tttacagttc tcacaatact ttaatataga tatagttcca caagtgtaac attcaaatat
      121 tacacaatcc tcattttcct ctactatttc aaacttttta ctatgttcac cacactctgg
      181 gcacttgtga ttatttacat tcataaatac tcctccttat tagtatagtt acatgtcaaa
      241 aacttaatta atgtattcta tattacttat tatatattct ataataaatc atattctaat
      301 ataataaaac aaattaaata aaaaaaaacc ataaatttta aatttatgga ttaattcttg
      361 taatgataac tctatttgag gatgtgaggg agggttatca ttacaatagg gtattatatt
      421 atggttttta agctagtctt tctacacctc atatgataca tatgctatat gaattttata
      481 tgatttttac ataaatattt tatgaaattt aaatttttta gtcattttga aatttgtaaa
      541 aagttctcta aaaatccaca gcatcataaa cagccaaaat ttaagtaaaa catcataaaa
      601 aaatttgtcc aataatttta acaagtaatc tcacctcatt ataattattg acacaaactt
      661 aaataaacaa tctataaatc ttctataaat cttaaacttt ccccctattg atacacaatt
      721 tctagtttgt caattttatt ttgcatttct aagaaaattt cttcaacaga attgttaaat
      781 atattgccta aatagattct tttatctttt tgaaatgaat aagattttac actaccatca
      841 gaagaaatat ataaatgact ccactctgct ccatattttg aaaacctctc ctttgtatct
      901 ttaaacctca tatcttctat ttttattgct ttttctctgt ctcctcttac tacagtgtga
      961 ccccttttta ttaaactggt tatttttata tcttctttta atttaatacg tgtatcatca
     1021 ttttttgaat atactttcca tatcacttta tgacgataat tatctaattc ctttattaaa
     1081 cttctatcaa tagtaaagct atctttagtt accacaatta aatcaaaatg gtctaacata
     1141 tattgaaata tttctctcat acttttattt tttaaaatat cctcaccaat aacctctata
     1201 aatctacagc ctttctcata catgcagttc aataaacttg tgatttcaga aaaataatca
     1261 tttgtattat tatctccatc tatttctata gtaaagtaca aaggagtaat gtatgattta
     1321 tttcctgtta tctcaaatac acttgatagt gtttgtatat catcttcaaa cgttaagtat
     1381 acattctcat aaaacataaa aaattccata actttttctt tcatattatg gatatttcta
     1441 tatctattgg ataatatatt tattatatcg tcagtagtat ttttcccatc acattcagcc
     1501 agtataacaa tttgcttcca acttaagcta tccacatttc cgatactttt tttatatcca
     1561 tcttcttcat cataaatagg accaattata atgttttctt ttggccacag atgaacccta
     1621 gttccatctt ttaatttcgg atatttattt tttacagact cataaaatct acttgtatca
     1681 cccatgattc tttactaccc cccatagttt aaatattcta tgctaaaaat agcactttat
     1741 ttttattaat tcaacaatta ttcaaaattg gtcattatat atgtttaaat ggatttaaca
     1801 tcaaccttat ttatatgtta ttgatgttta tatatgttat ataaaatctt atttctcata
     1861 cttcaatgta atcttaacta tttctggttt acaattaagc ctaattaaaa cttttgagcc
     1921 tcctaagcct ttatttaaaa tcatagtgct attatctttt tcatattttc cccaatcata
     1981 cttaggaaag aactctctat ttggagataa tacacctcca acaaaaggta ttcttattat
     2041 acctccatga acatgtcctg ctaaaatcaa atctgctccc cattttgcat atttttcaaa
     2101 gaaaaatggt gtatgtgcta aaagaatgtt atattcattt tcattaactt tacctagatt
     2161 gtcttctaca aaattgtcac ttagttctaa ttttttgtta ttagcaaata aatatggata
     2221 gcattccaat ggtataatta aaccatataa attaagatga ctattacctc tatatattct
     2281 tacagattca ttttccatat ttattatttt cttcgagtat agttccttaa aataatcctt
     2341 atatagatgt ttatactttt ttattaaaga tttttgttca tgatttccaa ttatatgata
     2401 tacttcatat ttttcaacca aattatctat taaatctaat gcaacttgaa aatttttatt
     2461 ttctccatct actaaatctc ctgttataaa tataacatct ggatttaatg attcaatttt
     2521 atctatcaat accttattat ttttatcaaa caccttatta tgtaagtcac tcacttgaac
     2581 tattttaaag ttatcaaatt cctttggaat ttttttgttt aaaatagtat attctgtcgt
     2641 ttctatatgc tgagtttcat acttatacag aatatacaat aatattaata ctaatactgt
     2701 cattgaaacc tttataatca tattctcttc ctctttattc tattctattc tattttatta
     2761 atattattat aatgctttta tgtttttttt aaaacaataa catcatcaca tacatataaa
     2821 tattttcgac attataaaaa taagttatcc ctttctaaaa ttttcattta ttttagaaaa
     2881 ggataactat aaaattatct ataaacctct atattgtgaa atatagtttt tcctttattt
     2941 aaatttttat atacatgctc taaagttcca tcaaacctaa tagaatgtcc agactctaaa
     3001 atataagttt tcccatccac attaaggtca agtttacctt ctattacgag cacatactct
     3061 tctaaaacat gactatgtgt agatgaaaca tgagtacagt tttcttcaag ctcaattata
     3121 aaaagttcaa accttctttg agggctaaaa gggtatattg gatataactt catgttccct
     3181 ttttgttcaa caataggctc tacttcttct ttttttataa cctcataggt atttgtattt
     3241 tcatttaata gtgtagtaaa atttatttta aatccagatg aaattttcca taaagtagat
     3301 actgttggac ttgattcacc tctctcaatc tgtccaagca ttgctttact gacacctgtc
     3361 atctttgaag ctttgtctaa acttatgccc atttcttgtc tcaataacct taaattttct
     3421 cccacattag gaaatttatt atccatgtta aaatcctcct gatagctatt tgtattctat
     3481 gatttttact cctgtaagtt ctactatctc ttttaatttt aacatatgat tgttacaaat
     3541 ttcatcaaat tcatatatat aatcttcaag ccctaatgcc ttatatttat cgattccaaa
     3601 tttatgatat ggaagtaact ccatttttcc tcctggaaca ttttgtttga caaataatgc
     3661 agtacttctt atgttttctt cactatcatt tactccataa ataattggca ctcttataac
     3721 catagattta ttcaactttg aaagcctaca tatattatct aatataattt tattacttac
     3781 tcctgtatat tctttatgaa tattatcatc catagatttt atatctacaa atatgtggtc
     3841 tattttctca aatacatcat ttaccttatt ccaatcaaaa tatccacaag tttcaattgc
     3901 aatatttatg cctttatcat aaaaaatatc aactaattct cttaaaaaat caatctgaag
     3961 tgtaggttca cctccagaaa aagttatccc tccattagat tcaaagaaaa atataaaatc
     4021 tttttcaact tcctctacta tttcttcaac actcatttct tcggtcatta tacaaatagc
     4081 atctttaaga catactttga tacactctcc acataaatca catttattat ttatttgtgt
     4141 tctatcatat agtgaactta tattttgagg acaaacatct atacatctat tacaggatac
     4201 acatttatct ttcataacac ctaattttac tatattactc caggaatctg gattagaaca
     4261 ccacttacat tttaatttac atccttctaa aaatatagta gtccttattc catctccatc
     4321 atttactgaa aaatgttgta cttttacaac tctacctttt ttactcataa tctacctcat
     4381 aattaaaaat tgctatgtga atttctagct attatagcat cttgcatttc tcttgataaa
     4441 ttaacaaatt gagtactata tccagcaact ctcactaata aatctttata ttcttctgga
     4501 ttttcttgag cttttaatag agtatcattt gatatcgtat taaattgtac atggaatcct
     4561 cctaaagcaa aatatgattt aatcatagat gttagatttc taagtcctct ttcagtttcc
     4621 accaaatctt gattcagtct taaatttaga agtgttccac cagtatgaac atcatgattt
     4681 atttttgatg cagatttcat tgctgctagt ggacttgttg tatcagtacc aacaaatgga
     4741 gaaactccct ctgtcaaagg ttttgtcgct tttcttccac aaggagttgc acctacgatt
     4801 tttccagttg gtacatagtt tgatataccc ataaatgcac tattaaattt agagccaaaa
     4861 atatccttgt attttctagt ttcagtataa taaaaatcag aaacttcaat tgctaatgaa
     4921 tctacataat cattatcatt tccatactta ggaacatcta acgcaagttt tcttaattct
     4981 tcataacctt cccaatcatt atttagtgct tttgttaatt cttctagcgt agtcacctta
     5041 tcctcaaata ctaatttttt tatagctgcc aatgaatttg aaactacacc taaccctata
     5101 ccagttaata caggacctat attatatttt gctcctcctt tacttaaatc aactccctta
     5161 tccaagcatc catctacaca agtagataaa aatggtcttg gtaccatttc tttatgaatt
     5221 tgttgagcaa cagtagttcc tataacagaa ctttttacaa gatatgctaa ttgctttaaa
     5281 aactcatcct taagttcttc aaaagaagta aattctgtga tatttttcgc atctagcccc
     5341 attttttctc cagtcaatct actcttacct tcattcatag catattctaa agctgctgca
     5401 aaattgatat ttacggctga agtccactgt cctgtttttc taaaatgagg aactacacaa
     5461 ccacagttac tccaatctct agcatcttct gcatcatatc catcttgtag caacatttgt
     5521 gcccctgcac tatcgctgtg tatagcaggg aaaccagttc cttgttttac caatttagca
     5581 actgccatta cgaaattatc tggtgctcct tgactcaccc taacacttaa tcctggctga
     5641 tgagttttta cactttctgt agcctttaaa cacatatatg agatatcatt cgttccatca
     5701 gtaccatttc tctttttacc accaacagta agattttgga attgattata tcctgcaaaa
     5761 taatttgcag tatttgctga tatggtccat acccattctg ataacttaat ccaaagtgct
     5821 tctatcaatt cttgagcttc tgattcagtt attttccctt ctctagcatc attttcataa
     5881 tatggataca tatattggtc aaatctacct aaatttaatg ccaatggatt ttctgataga
     5941 ataccaccta attgaacaaa ccacacaaat tgtattgctt cataaaaatt agttggagga
     6001 tttgctggta cattcataca tatctctgaa atttttataa gttctaactt tctcttttca
     6061 tctgtttctt gcttactcat ctcttttgca agacttgaat atctttgtgc caatgtcata
     6121 ataccctgtg caactataga aacagatttg taaaaatcta tcttttcaat attttctgaa
     6181 actgatatat ctaattcttt aattttttta tctgcatctt ccttaatccc tttatatcct
     6241 tttacaaaca atatatcttg ataatctggt gttacttcac caactgcctg tctccactta
     6301 gaatcattat ctattattcc tgtatctact aatatcttag ctgtatcttc tgggattctt
     6361 gctaagaaaa cttcttccaa agattttccc ttccaatatg gaaatatttc ttttcttata
     6421 aattctattt gttcttttga tattacatat gggtcttgag ttcttttatc aaaagtatcc
     6481 atttctttgt ctacccattg ccaagaaaac tctggtgtca atatccctgt tcttctaaat
     6541 tttcctgctg tacctactat taattcattt tcaaagattt ttataggaag ctctttacat
     6601 gtctccaaaa aagctttagc tcttcttata cagatagctt ctccctcagt ttttttatgt
     6661 gattctgtaa aaattctagc cctttcacaa catatcatag gtttagaatt aatatattct
     6721 tgtctaaatg cttctattct attagtttga ctcatttaaa atcccccttt ggataatata
     6781 tactacattt ttgtatgtta taacatactt taattatatt atactgtcct ttagcataaa
     6841 atggaagcaa aataacttat tttttttcat ctaccttgaa cacatatata ttaatacttt
     6901 ttttgttaaa tttataaaat ctaaaaaaat tttctatgat atattttcta tattgttata
     6961 gttatttcta atatactaat gatttgaaat atcaaatttg tatactatac ttagttttaa
     7021 aaccttggtt gattgtttaa ctttgttaac ttcaacatgt atatccttca tataaggaag
     7081 ctctacaaca cctactccct tattaaattc ttcttcaacc atagaaaaaa tttctttctc
     7141 agttaagttc gtattagtca atgacttaaa taaattgtat ataaatttaa tatatttatc
     7201 actctcagat aacttatcat cttctggata ctctttagca cagagaatat ctattgttac
     7261 ttttccttct ttatcgtctt cagacataga atagaataaa cttcgaatat ttttttcagg
     7321 tggtttttca gcaccattta tagataccat aaagctacca tctgattttg agtcagtaat
     7381 actataacca ctgtctatta tagctttcga aaatctttga gataaatctt tttttacttc
     7441 ttctctacct ttcgcatcat taatcttttg tgagtaatta ttaaatagct caatagattc
     7501 ctctttttta tcttcaactt gttctttcaa tgaaccttta ctaatttgtt ctgagctact
     7561 taagttctta tcagatacag gagacataca tcctaccatc aatgctaagc taagatttaa
     7621 agccacaatt attgccactc tatttatatt cctttttact ttcatacaca atcctcccat
     7681 cacactaacc gctctaaagt attactcatt gcactagaat atgtttatat tctctttata
     7741 ttaaagattt attattcaat acatatcacc ttcctatcca attcttataa cttactatta
     7801 gtaaaattat aagcttctta atacagaaat aaggatgtta tcctttgtta ttaaaagata
     7861 acatccttat ttttttgtat caatacatat gtcccaacta gtaacttaat cgccccaact
     7921 gtcagtatct tggtccaatg gtatatatat atttagtata aatttatcga tactattttc
     7981 aaataataat tctccattat atttttgtaa agaagattta atactctgaa tacctattcc
     8041 atgtacgaac ttatcctttt tgtttgttaa caatatattt ttattaaaag taagctgatt
     8101 tagtttacta ttttcacatt taagtacaaa aaatgacttt gttatagttc ctttaattct
     8161 aatatattta ttaatatttt catccactat tttatcacat gcttctattg cattatccaa
     8221 aatattagaa aatatgcttg acacatctat aggttttaca aaatttactt ttgaaaaatt
     8281 tatagagcaa gtaaatttaa ttcctttttt taaacatatt tcactctttt cgttgattat
     8341 aatgtctaat atcatatttc ctgtattata gatatattta aatgttttta tttcatcttt
     8401 taaattgtta acatattcat caatttcttt gctattattt ctaaggttgt ctatacaata
     8461 aatgtgatta tttatatcgt gatatagttt cttaactcgc atatgagatt cttgaatact
     8521 taaataatgt gcatattgca tatctaactt attttttatt aattcagctt gggattttaa
     8581 tctattctct tttattattt ctttaaacaa tagtataaga aatatactac tagctcctac
     8641 caatttggga ttaacaaaat tatcaaaata gtatccttct gaatgtaatg acccaaaccg
     8701 ttctaatttt tttaatacat aatttacaaa catattcatt gtattagcta ataaggcaaa
     8761 taataaatat acatagtttc tcttatcttg gctaattaat tttatagact tcctaaaaaa
     8821 gctaaatata agatatataa gaataattat taaattacta tatatctgaa tttttataga
     8881 ttctaagcga ggtactaata aggattcttt agatgaaata acattacctg taattgcaaa
     8941 aaatagtatt ctatatataa cagcatccaa acatttataa tatacactaa atagataaaa
     9001 gaaaacggaa tacttaatat aacctatctt ataaaaa
//
LOCUS       DAEMAE010000149         9023 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEMAE010000149 DAEMAE010000000
VERSION     DAEMAE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.151"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..730)
                     /locus_tag="KPH93_002689"
     CDS             complement(<1..730)
                     /locus_tag="KPH93_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689491.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVK"
     gene            complement(748..1683)
                     /locus_tag="KPH93_002690"
     CDS             complement(748..1683)
                     /locus_tag="KPH93_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1689492.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIDIKNNGPKIHNVESIFWDGFSTKEGQDRGY
                     GLHLVKNILDKYNYSILVNSDDFLTEFVILIPKYY"
     gene            1891..4476
                     /locus_tag="KPH93_002691"
     CDS             1891..4476
                     /locus_tag="KPH93_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689493.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QASVDRFRRELGPDHVYFKDIKRNQLELIKSSKDIKNLGITSHYGYTDINEKLPINIE
                     YVNKNYLTSHSKLIKGHFPKANNEVVVEKWVLNSMGLKPEINQDITFKLFKKEKPETF
                     KVVGILEDRAIQKKQGMCEIFLNINESKLDNFSYVYVEFNEGSDINTNINNIVKNTML
                     DKNSVGKNKTLIEATMENGTLDGSSKYIAIAMSIFSGIVIYSIYVISIYQRVQEYGIL
                     RAIGATNFRIFKLMFYELFILALIAIPTGICTGMVGAQIFNKTAGNIQFEGDVRVTPF
                     VIPDKVIFLAIGCIILTILIISFFTYLKIRRISPIDAIRKTFGTDKNISKVNSLISKL
                     TSNMSATKSISTKNILRNKKGFIIIILSMSIGGIMVIKENYKYSFSDTQNKDSQERAY
                     MNADFILSNFTFKLNENYKANSNADINGLNDNQIDKIKNIDGIDKVKTASILNTRIEL
                     DKINKLDYYNIISSSPYYNEFPILTKNKTTGKYTLAQRLRGYNDEMINSLEKYLVSGS
                     INLEKMKKENLAIVYVPQISKTNKYSKSYIPGVGTSVVDIKVGDTIKVKYPKGEIDTD
                     LYMRQKDNYEYLEYEFKVGAIVSYPFADNNSYSGDEGIDVITSSEYLKKLTGVNKYNT
                     VYIDLKENANIKKINTLLGKIGSEVPGTTTVDMSVEKENSDNMTARAMIYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISDRRFKNMILYEGILYGTLSSVITIVA
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYIIVILANIIVGILATYIPLRKINKISIVES
                     INITE"
     gene            4488..5159
                     /locus_tag="KPH93_002692"
     CDS             4488..5159
                     /locus_tag="KPH93_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689494.1"
                     /translation="MPILETINLGKIYGKKETSVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            5186..5527
                     /locus_tag="KPH93_002693"
     CDS             5186..5527
                     /locus_tag="KPH93_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689495.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKFIKK"
     gene            complement(5887..8529)
                     /gene="adhE"
                     /locus_tag="KPH93_002694"
                     /gene_synonym="adhC"
     CDS             complement(5887..8529)
                     /gene="adhE"
                     /locus_tag="KPH93_002694"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG1689496.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVAKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAKEGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTASSGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLEGLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         3224 a         1327 c         1201 g         3271 t
ORIGIN      
        1 ttttaaccct ataaaatcta tacagaaaat ctttttctag agatttgttt aatatggcat
       61 tttcttgaaa atttataaac cttatctcta atgaagattt atttataggt ataaccttat
      121 gtataaatct tagatttact atgtattgtc tatgagtcat aaagaaatta ggaggtaggc
      181 gtttttttaa atcatcaaag ttctcataaa actttattat atcttcatct ttagtgtgta
      241 gattaattgc tcttgaattt ttctcaaaca taactatatc atcaaaatta atcatatgta
      301 tctgcttcct tattttatag acaaacagag tgtcttctat aaaattatta ttagcaattt
      361 tatgagagtt aatatggtct attgctatgt taattgaatc aagtattttt tcttttttaa
      421 atggcttaac tacaaaatct ataggatgca ctttaaagct ttcaagacta taatcagcaa
      481 agcccgttac aaatactata tgtatatcct tatctataga aagaatttct ttagcagaat
      541 caatcccatt tttatatggc atatcaatgt caataatcaa caaatctatt ttctcgtttt
      601 ttataaactc aattgcctct ataccatcct taactttttt aacataatct acatatccag
      661 attctttaac tatatttgct atatcatctc ttacaatttt atcatcatcg caaatcaaaa
      721 cttttatcac agactttctc ctcctaacta ataatatttt ggtattaaaa ttacaaactc
      781 agttaaaaaa tcatcactat ttacaagtat actataatta tacttatcta atatattttt
      841 aactaggtgt agcccataac ccctgtcttg cccttcttta gtgctaaaac catcccaaaa
      901 tatactttca acattatgta ttttggggcc attatttttt atgtctataa tatactgaaa
      961 ttcatcttca tatgcatgta atcttatata cttcttttct ttttttatag tagacaaaga
     1021 ctctattgca ttatctatta tgtttaatag tactgaactc atatcattag gttcaatttc
     1081 taaattatcc ataaaatctt gaatgtagac atcaaaagat atatcctttt caagacattc
     1141 ctcgtatttt aatgaagcta tagcatctaa ataagatact tttccatacc ttgaattttt
     1201 tgatttaaaa ctatttgata tattcattat atattctttg gcatcttcat ctagcccagt
     1261 atttaataga gaatacacca cttgcaaatg tttcaaatag tcatgttttt gacttcttaa
     1321 ttttatgatt atattatctt tatgatttat tttactatct agtttttgta gtttatattc
     1381 ctgttcatta ttattattta tcaatcttat aactttaaag cctgtataaa aacttaaaat
     1441 aattaaaacc aaaaaaagta taatatttaa tactccttca agtaaaaaac tatatcctaa
     1501 ccttaattgt atatatttag tgccctccat aaataaaatc aatgcaaaac acgatataaa
     1561 aaatgttaat atgcctcttt ttaatatgtc taattctttg taaaatttat acttattcat
     1621 tataagattg ctaaatataa acaaagttag tattactata ggaaatacaa aattgttatc
     1681 catgattccc ctcccctcta caatttatat agggattata tcattttaaa aaaattatgt
     1741 aaaaagttaa aatttacata cagattaatt aaacttgtaa aaatacagat gaatttgtac
     1801 aattaactac ttaaaatttg aaaatactta ctttgatata tattttagat aaatatataa
     1861 ttgatttagt taagaaagga agtggtaaat atgaaagata atttgaagat agtactaagg
     1921 tacataaaaa gttataaagc tagatcttta gcaattgctc tgagtataat tcttgctaca
     1981 tcacttattg ttggtattgg tactctttca agaagtgctc agcaagcaag tgtagataga
     2041 tttaggcgtg aacttggacc tgaccatgta tattttaagg atataaagag aaatcagcta
     2101 gaactcataa aatctagcaa agatattaaa aatttaggta taacatctca ctatggttat
     2161 acagatatta atgaaaaatt acctataaac atagaatatg tgaataaaaa ttatttgact
     2221 agtcattcta aacttataaa aggtcacttt ccaaaagcta ataatgaggt ggtagttgaa
     2281 aaatgggttt taaatagtat gggtttaaaa ccagaaataa atcaagacat tacttttaaa
     2341 ttattcaaaa aagaaaaacc agagacattt aaagttgtag gaatacttga agatagagct
     2401 attcagaaaa aacagggtat gtgtgaaatt tttttgaata tcaatgagtc aaaattggat
     2461 aacttttctt atgtttatgt tgaatttaat gaaggtagtg atataaatac aaacataaat
     2521 aacatagtaa aaaatactat gttggataag aatagtgttg gaaaaaataa aacgcttata
     2581 gaagctacta tggaaaatgg tactttagat ggctcaagta aatatattgc aattgctatg
     2641 agtatatttt ctgggattgt aatatatagt atctatgtta tatcaatata ccaaagagta
     2701 caggaatatg gtatcttaag agctattgga gctaccaact ttaggatttt taaacttatg
     2761 ttttatgaac ttttcatact tgctttaata gcaataccta caggcatatg tacaggtatg
     2821 gttggtgcac aaatatttaa taagactgcg ggaaatattc aatttgaagg agatgtaagg
     2881 gtcacacctt ttgttattcc agataaagta atctttctag caattggatg tataatactg
     2941 acaatactaa ttataagttt ctttacgtat ttaaaaataa gaagaatttc tcctattgat
     3001 gccataagaa aaacttttgg aactgataaa aatataagta aagttaattc tttaatatca
     3061 aaattaactt caaatatgtc agcaacaaaa tctatatcta ctaagaatat ccttagaaat
     3121 aaaaagggat ttattataat tatattatcc atgagtatag gaggtataat ggtcataaag
     3181 gagaattaca aatattcttt ttcagataca caaaacaaag atagtcaaga aagagcatat
     3241 atgaatgcag attttattct atcaaatttt actttcaagt taaatgaaaa ttataaagct
     3301 aattctaacg cagatataaa tggtcttaat gataatcaga tagataaaat aaaaaatata
     3361 gatggaattg acaaagtcaa aactgctagc atcttgaata caagaataga attagataaa
     3421 ataaataaat tagattacta caacattata agttcatcac cttattataa tgagtttcct
     3481 atattgacaa aaaataagac tactggcaaa tatacgctag cgcaaagatt aagaggttat
     3541 aatgatgaga tgataaattc attagagaaa tacttagtga gtggaagtat aaacttagaa
     3601 aaaatgaaaa aggaaaacct ggctatagta tatgtcccac aaatttctaa aacaaataaa
     3661 tacagcaaga gttatattcc tggtgtagga acgtccgttg tagacataaa agtaggagat
     3721 actataaaag tcaaatatcc aaaaggtgaa atagatacag atctttatat gagacaaaaa
     3781 gataattatg aatatctaga atatgaattt aaagttggag ctattgtgag ctatcctttt
     3841 gcagataata attcatactc tggagatgaa ggtatagatg taataacgag tagcgaatat
     3901 ctaaaaaaat taactggtgt aaataaatat aacacagttt atatagatct taaagaaaat
     3961 gcaaacataa aaaaaataaa tacactttta ggtaaaatag gaagtgaagt tcctggaact
     4021 acaacagtgg atatgtcagt ggaaaaagaa aactctgata atatgacagc tagagctatg
     4081 atttatgctt atggcatagt agcagttatg ttcataatta gtgtatttaa tataataaat
     4141 aacataagtt ataacttaac ttcaagaact agcgaatttg gaatgttgag ggctattggc
     4201 atctctgata gaagatttaa aaatatgata ctctatgagg gaatattata tggaacactc
     4261 tcaagtgtaa ttactattgt agcaggtctt ataatacaat ttagaatgta ttacacgtat
     4321 aattttgtat catatggatt aggattttca atagattaca aaatatatat catagttatt
     4381 ttagcaaata taattgttgg tattctagct acatatattc cattaaggaa aataaataaa
     4441 attagtatag ttgaatctat aaacatcaca gaataggagg ggtaattatg cctatattgg
     4501 aaactattaa cttaggtaag atttatggaa aaaaggaaac atctgtacat gctctaaagg
     4561 atgcaaattt aaaaataaat aaaggcgaat ttgttgctat aattggacca agtggaagtg
     4621 gaaaaagtac atttctacat ttagtaggtg gtcttgaaag accaagtaat ggtactataa
     4681 aggtagatgg taaagatata tgttgtcttt cagataaaga attagcaaga tatagaagac
     4741 aaaaggtagg atttgtcttt caacagtata acttaattcc tgtattaaat gtaaaggaaa
     4801 atatagaatt accactaaag ttagataata aaaaaatcga taaagaatac attgatgacc
     4861 ttataaattt attgggtctt aatgaaagga aaaatcatct tccgaatcaa ttatctggtg
     4921 gtcaacaaca aagagttgct attgcacgtg ctttatctgc aaaaccaagt ataatcctag
     4981 cagatgaacc aacaggaaat ttagacagta aaactacaga agaagtcatg gatttactaa
     5041 aaagttctat aaaaaaatat aaccaaacac ttataataat aacacataat gaaaatattg
     5101 ctagaaaagc agatagaatt atatctataa tagatggaga gcttaaatta acactctaaa
     5161 actatttata gaaaagagga tttaaatgtt attgatttta ggtaatttaa atttaaactt
     5221 ggattttgac tacagaataa taagagaaga aaatgacgat gtagatatat tcatagatat
     5281 taattatcgt agcttagata tagatactga tggttctaac ttatttaatt ctagaataca
     5341 atttccattt gtaagagcat tgattctaag gctaaataag aacaatcaat gtatgacaat
     5401 tcatttgtta agagacatag atttattctc tgcttttgcc aactttgaag tggattatac
     5461 tgactctata ataaatataa aaaatcaaaa tgagaaagta attttaaata aatttatcaa
     5521 aaaatagtaa gggggcctta ataggcccca attctttttc ttttctaaac ttattatatc
     5581 atgataaata aattagtcaa tagaattttc tgaatatttt tataattttt tcataaagtt
     5641 ttttatatat tatgctttaa aattatatgt aataaaaaaa ttttccgact aattttaaag
     5701 ttaattgatt aaatgcacat attcaaaatt accattaaat tactgacttt ttatcatttc
     5761 cattcagaat ctaagttata atctattatt tataactatt tatacgtgtc tttaataaaa
     5821 ctctatctta ttcaaatcta ataatttata tacaaaccca atagaaccta tgctgtacaa
     5881 tgaatattat ttaccataat aaacttttag atatatctct tttaattcac tcatcaatgg
     5941 atatctagga ttagcacctg tacattggtc atcaaaagct tgttcaacca tagcatccag
     6001 tccttctaag aatttagatt cttctacccc agcctctttt atagtcttag gaagacccac
     6061 ttttgccttt agttcatcta ttgcttttat aaggttatct actttttctt ggtctgtatt
     6121 tcccttaatt cctgcaaaag aagcaaattc agcatatctt tgtatacaat caggatatct
     6181 gtattgtgag aatgcaccca tcttatttgg agcttctgca caattaaact tgattacttc
     6241 atttattaat aatgcatttg caacaccgtg ttgaacatga tggaatgcac ctaatttatg
     6301 tgctaaagag tgacatattc ctaagaaagc atttgcaaaa gacattcctg ccatagtaga
     6361 tgccattgcc attttttctc ttgcttcttt atcattcttt ccatttttat atgcacgagg
     6421 taaatattca aatattattt tacctgcttg tagtgctaaa ccatcagctg gttcagttct
     6481 taacatagat acatatgctt ctagtgcatg tgttaaagca tcaacacctg atgacgctgt
     6541 tagtcttggt ggcatctcca tcatcatatc agcatcaatt atagccatgt ttggcattaa
     6601 ttcataatct gctaatggat attttacgcc tgaatcttgg tcagttataa ctgcaaatgg
     6661 agttacctct gaaccagttc ctgctgaagt tgggatagca gcaaagtatg ctttttcacc
     6721 catcttaggg aatacatata ctctttttct tatatccata aatctcatag ctaagtcttg
     6781 gaaatctact tctggatgct catacattac ccacattatc ttaccagcat ccattgcgct
     6841 acctccacct acagctatta ttaaatctgg ttggaaatca gccatagctt tagccccttc
     6901 ttttgcacaa gctaatgttg ggtctggagc tacatcaaag aatgtagtgt gcttaattcc
     6961 catctcatct aataaatcag ttaacttctt agtgtatcca ttattatata ggaaagagtc
     7021 tgttactata aatgctttct ttttatccat aacatcctta aattctcttg ctgctacacc
     7081 taaacatcct tttttgaaat aaactttttc tggtgctctg aaccaaagca tattctctct
     7141 cctttcagct actgtcttga tattaagcaa atgttttact cctacatttt cagatactga
     7201 gtttccaccc caagaaccac aaccaagagt aagagatggt gccaatttaa agttatataa
     7261 atcacctata ccaccttgtg atgatggagt attaactaat actctacaag tcttcatagc
     7321 atttacaaac ttttctactc tctctggatg atttatagag tctgcatata atgaagatgt
     7381 atgaccatat cctccatctg caactaattt ttctgctttg gcaactgcat cttcaaaact
     7441 tttagacttg tacattgcaa gtacaggaga aagtttttca tgtgcaaaag gttcatttaa
     7501 ttcaacagat tctacttcac caattattat tctagctgtt tttggaactt ctacatcagc
     7561 cattttggct attacgtgtg ctggttgtcc aactattctg gcatttaatg aaccatcaat
     7621 tattactact tttgctactt tttcagtttc ttctttattt aggaaatgtg ctccacgata
     7681 tttaaattct tttttaactt cttcatatat tttttctggt actattacgg cttgctcgga
     7741 agcacatatc ataccattat cgaatgattt tgatactaag attgaactta cagccatttt
     7801 tatatctgca ctctcatcta ttattgcagg cacatttcca ggcccaactc caacagctgg
     7861 tttacctgaa gagtatgcag atttaaccat tccaggtcca ccagtagcta atataaggtc
     7921 tgcctctgcc atgacaacat tagttaattc aatcgaagga acatctaccc aacctataag
     7981 accttttggt gctcctgcct taactgctgc atcatgtaca actctagctg cttctatagt
     8041 acagttttta gctcttggat ggggagagaa aattatacca tttctagttt ttagtgctat
     8101 tagggatttg aatattgcag ttgatgtagg attagttgtt ggtataactg ctgataaaac
     8161 acctactggc tcagcaattc ttgtaaaacc aaatgcttca tctttttcta tgacaccaca
     8221 agttttagta tctttataag cattatagat atattctgaa gcaaaatggt tttttataac
     8281 cttatcttca acaataccca taccagtctc ttccactgcc atttttgcta gaggtattct
     8341 ttgttgatta gctgcagttg aagcagctaa gaaaatctta tctacctgtt cttgcgtaaa
     8401 tgttgcaaat tctttttgtg ccaatctaat ttcttctaat ctcattctta atgtttcaac
     8461 actgttgaca atttcaggac tacatacttc aacatttgat ttttctacta ccttttcttt
     8521 tttctccata ctaaaaacct ccctaaaata attatgcatc tctaattttt ataagtctta
     8581 aatgcatcat ctatattttt aacttatact taaaataatt ttatagattt catctctata
     8641 ccttagattt tatttatttt actctcttag gtcaagaagt ttttttctga atattttgtc
     8701 aatttatttt ttaacaaata tattattttt tttactttta ctaaaatttt ttatctaaat
     8761 gagatatatt tcatttgaat aatttacttc gactaaataa aatactaaaa tgataattta
     8821 tatctttcat tttttttact tattaaattt tatatgttat ttgcaggtta tttgtataag
     8881 tagtaaataa aatttttgtc gtatttttat aaaataacgc ttatttttga cttgtgtgca
     8941 atttttcttt ttataacaac tgtatggttt taaaaaatac acaaactaaa attattctaa
     9001 tttccataag ttatttttaa gtc
//
LOCUS       DAEMAE010000150         8972 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEMAE010000150 DAEMAE010000000
VERSION     DAEMAE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.152"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1664
                     /locus_tag="KPH93_002695"
     CDS             159..1664
                     /locus_tag="KPH93_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG1689497.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMAKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2055..3152
                     /gene="dinB"
                     /locus_tag="KPH93_002696"
     CDS             2055..3152
                     /gene="dinB"
                     /locus_tag="KPH93_002696"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG1689498.1"
                     /translation="MKLHRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKSIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVDPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSISLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3415..4551
                     /locus_tag="KPH93_002697"
     CDS             3415..4551
                     /locus_tag="KPH93_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689499.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4544..6091
                     /locus_tag="KPH93_002698"
     CDS             4544..6091
                     /locus_tag="KPH93_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG1689500.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKVNEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6425..7291
                     /locus_tag="KPH93_002699"
     CDS             6425..7291
                     /locus_tag="KPH93_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1689501.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     ITNPVVPAITIESLIALIITLFINIFVCMYEYRVGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7432..8574
                     /locus_tag="KPH93_002700"
     CDS             7432..8574
                     /locus_tag="KPH93_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG1689502.1"
                     /translation="MRYVEYGKTGKMISVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMKPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKA"
BASE COUNT         3454 a          957 c         1592 g         2969 t
ORIGIN      
        1 ttatttacca taaaactatc ttgaaaaaaa ttaatcttaa ttgtataata ttactttgag
       61 tgaaaaagta ttaacatttt tttataagat gttttactaa aaaaatatat ataaatatac
      121 tgttatcaac aagaaattta atttagtgag gtgttaagat gaaagtagga tttgaccaca
      181 aaaaatattt agaagagcag tctaaatata tcttagagag agtaaataat tttgacaaac
      241 tttatttaga gtttggagga aaattgatgg gagaccttca tgcaaaaagg gtacttccag
      301 gatttgatga aaatgcaaag ataaaagtat tacaacatat gaaagaaaaa gttgaagttg
      361 taatttgtgt ctatgcagga gatatagaaa gaaacaaaat aagaggagac tttggtatca
      421 cttatgatat ggaagtttta agattaatag atgatttaag aggttatgaa ctagatgtaa
      481 atagtgtagt aattactaga tttgaaggac aacctgctac aactgtattt ataaataaat
      541 tagaacgtag aggaattaaa gtttataagc attatccaac aaagggatat ccgtcagatg
      601 ttgagactat agttagtgat gagggatatg gagcaaatga atatatagaa actacaaagc
      661 caatagtagt agtcacagca ccaggaccta atagtggaaa acttggtact tgtctaagtc
      721 agttgtatca tgaaaataaa agaggtaatg tagttggata ttctaagttt gagacattcc
      781 cagtttggaa tgtgcctgta aaacatcctt tgaatatagc ttatgaagca gctacagttg
      841 acttaaaaga tgtgaatatg atagattcat tccaccttga aacttatggt gaaatgtcag
      901 taaactacaa tagagatttg gaattatttc cagtgcttaa aaaaataata gaaaagataa
      961 ctggaaaaga atcaattttc caatctccaa ctgatatggg tgttaataga gttggttttg
     1021 gaataataga tgatgaagta ataagaaaag cttctataga agaaataata agaagatact
     1081 ttaaaactgc ctgtgagtat aaaaagggac aagttgataa aggtgcttat gatagaatga
     1141 aaatgattat ggaagagctt aacttaaagc cagaggatag aaatgttgta ttacctgcta
     1201 gaaactacag taataaattg aaagaaagtg ctgacaagaa tgatacttgc cctgtagttg
     1261 cattagaatt agaagatggt acaatattaa ctggtaaggg ttctgattta atgaatggaa
     1321 cagcagcagt agtattaaat gccattaaac atttagccaa tatatctgat gatatgcact
     1381 taatatcacc tgtaatatta gaacctatca taaacttaaa aacaaagact ttagctagta
     1441 gaaatgtttc acttagttgt caagaggtgt taactgcact tagtatttgt gctgtaacta
     1501 atccaactgc acaatttgct atggcaaagt tagcattatt aaaagggtgt caagctcatt
     1561 ccactactat attaaataga gatgatgaac aaacatttag aaagcttggt atagatgtaa
     1621 cttgtgaccc agagtatcct tcacaaaatc tttactatag ttaaaattgt ataaaaaaat
     1681 atgggctgtt ttaaagtaat agtttgagca gtccattaca attataataa aatcagtagg
     1741 ctgtcttgta taagacagtc ttttgttttt aaaacatccc ataaattaaa tatatatact
     1801 gttatccata aggaatttta aatttagttc cttggaatac ctattgtaaa acttttatat
     1861 agtttttgca agtgttttta aggatttttt ataaattata ttgaagttaa tggattatag
     1921 atagttttat ttatggaaaa tatgaatata taaagaaaat aatgaaaaaa taccaaaaag
     1981 aaacataagt ttggtataat taaaaagtaa tataaataaa atttaatcta tttatacttt
     2041 aaaaggtgat aatgatgaaa cttcatagaa aaataattca tatagatatg gacgcttttt
     2101 atgcatctat agaacaaaga gataataaaa aattaaaagg aagacctgtt attgttggtg
     2161 gaaatccaca aagcagaggt gtggttgcaa cgtgttctta tgaagctaga aaatttggta
     2221 ttcattcagc tatgccatca gctgtagctt acaataggtg tccatatgca gtttttgtaa
     2281 gacctagatt tgatgtttat aagagtgtgt cagagcaaat aagagatatc ttttatagat
     2341 acacggattt agttgaacca ttatctttag atgaagctta cttagatgta acaaaaaata
     2401 aaaaaagtat tgattcatca attgagatag caaagcaaat aaaaaaagat atatttagag
     2461 aagttggtct tacttcttca gctggtgtgt catacaataa gtttcttgct aagattgcat
     2521 cagacttaag aaagccaaat gggttaactg taataaccga agaaaatgcc caggactttt
     2581 tagataaatt acctgtgaat aaattttttg gtgttggaaa agttacttca aacactctta
     2641 aaaatttagg tataaaaact ggatatgatt taaggtgttt aaatttattt gaacttgaaa
     2701 atatattcaa aaaaagaggt tatgaattat ataaatttgc tagaggaata gacgatagac
     2761 cagtggaccc aaatagggtt agaaaatccg ttggagcaga gactacatta agtcataatt
     2821 tggatattga tgaagaagaa acacgcaaca ttcttgatga actttgtgaa gaagtatgtc
     2881 atagattaaa aagctctgag aagtttggaa aaacattaac attaaaaatt aaatatgagg
     2941 attttacaaa gataactaga agcattagtt tagaacacta tattgatgaa tataatgata
     3001 taagaagtgg tgtagataat ttgttgagaa atgttgaggt taatggaaaa caaattaggc
     3061 tattgggagt gacaatatca aacttaagcg ataaaaaaga aacttataaa gatattacat
     3121 tatttgaata catggatagt atccaaatgt gagttttgtt taaatgaaat aacactagct
     3181 ttaatactat ataattgagg ctagtgttat tttatgaata tttttatact ttttgttttt
     3241 tgacattata tattatatac tatatataaa tactttatat ttgtagcaat attcagacaa
     3301 atattgacct aaaagtcgta ctttgtttac ataaataaaa tagtatgata taattataaa
     3361 aacaacttat aatattggag aaggttgctt gttcgaaaaa ggggatgata aattatgata
     3421 gagataagaa atgtaacaaa aaaaatagga aataatgtta tattagatga tatttctcta
     3481 gttgtagaaa caggtacatt ggtagttttg attggttcga gtggttgtgg aaagactact
     3541 actttgaagc ttataaacaa attaataaaa cctacatctg gtgagatata tattaatggc
     3601 aaaccaattt ctcaggaaaa tgaaatagag cttagaagaa aaataggata tgttatacag
     3661 aacacaggcc tattccctca tcttacaata aaagaaaata tagagctaat tccacgccta
     3721 aaaaaggaaa aatccgttga agaaattgaa aaaagaacgt tgcaactact tgagatggta
     3781 ggacttgata gtgatgaatt tttgaataag tatccttcag aacttagtgg tgggcagcaa
     3841 caaagaatcg gtgttgcaag agccattgct actgatgctg aaataatatt gatggatgag
     3901 ccttttagtg ctttagaccc aataacgaga acatctttac aagaacaatt attttctctt
     3961 caagatgaat tgaaaaaaac tataatattt gtaactcatg atatggatga ggctttaaag
     4021 atagctgata aaatttgtat tatgaaggat ggaagaatag cacaatatga tactcccgaa
     4081 aatatactta gaaagcctgc aaatgacttt gtaaaagatt ttataggaga agatagagtt
     4141 tgggataatc cagaatatat aaaagcaaga gatattatga taaaaaatcc tattgctgtt
     4201 aattctacta gaactgtaac tcaaggaata gaaattatga gaacgagtaa ggttgatagc
     4261 ttactaatta tagatagagc aaaaacatta aaaggtattg ttactgtaaa agatatgaaa
     4321 gatattgatg ataagtcaat attacttgct gatattatgt caagtgagcc tttgcatgta
     4381 aatgaaggtg ataatttagt agaaatacta aatgtaatga atcgtaattc ggttggatat
     4441 attccagtta tttctgatga aaataaactg gtagggctta ttactagaag tagtttgctt
     4501 tctgttttaa gtgaacaatt cttagaaatg gaggtgagtg ttcttggcta atttttttaa
     4561 ctttatacta ttacaaaaag ataagattat agaactttta atacaacata tgagtctaac
     4621 tgttacatct attttaatag ctataatagt tggtgttcct cttggaataa ttatttcaag
     4681 aatctcatct cttagaaagt ttgtacttgg atttgtaaac ttagtccaag cagttccgtc
     4741 tatggcactt ttgggtcttc ttgtacctat acttggtata ggaagtaaac cagccatatt
     4801 tatggtagtg gtttattcat tactaccaat tgtaaaaaat acgtatatag gtataacaag
     4861 tattgaccca gtagtattag aatcagctaa aggtattgga cttacaagaa atcagacatt
     4921 atttaaaata caattccctt tagcgcttcc tataataatg ggtggagtta gaatatctgc
     4981 agttacagct gttggtttga tgacccttgc agcatttata ggtgctggtg gtttaggata
     5041 tttagtattt tcaggggttc aaactgtaaa taataatatg attttagcag gggcaatccc
     5101 atcatgtata attgcactgt tagttgatta tttatttagt aaaattgaag ttgcagttac
     5161 tccaaaagga ttaaatccaa aagcacctaa gaaaaattat atagcactta aagtaatatc
     5221 tgtagtgctt gttgtttcaa tgttgttcgt tgtttttagt tctagttttt caagtaaaaa
     5281 agatacaata actattggtt ctaaagatta tacagaacaa cttatacttg gtaatgttta
     5341 tgctgaatta gtagaaaaaa atacaaatct taaagttaag aagaatttaa atctaggtgg
     5401 ttcatctgtt gcatttaatg caattaaatc tggtgagtta gacatgtatg ttgactatac
     5461 aggtacactt ttggtaaatg taatgaaaca tgctccgatt aaagatgctg atgaagcata
     5521 taatgttgta aaagatacta tggaaaaaga aaatcagtta actttacttg accctcttgg
     5581 atttaataat acttacacat tagccatgat gcctgaaact gctgaaaagt atggaataaa
     5641 tactatttct gatttgacta aatatggtaa agaatttaca ttctcaccaa ctttggagtt
     5701 tgaaaataga gaagatggtc ttgtaggact ttctagagat tatggtttaa aattcaaaga
     5761 tgtaaaagca atgaatggaa gtttaagata tacagcttta gataataatg aatctcaggt
     5821 tatagatgca tttttaactg atggtcttat taaaaagttt aatcttaaga tattagagga
     5881 cgataaaaac ttctttgtac catattatgc agcaccatta gtgagagaag atactttgaa
     5941 gaaatatcct gagttagaaa aagttatgaa tatgctttca ggaaaagtaa atgaagaaac
     6001 tatgagagaa cttaactatc aagtggatga acttggtaaa tcaccagaag aagttgctca
     6061 ttcattctta gttaaagaag gcttagtata aaaaattaat agtttatatc aaaaatttaa
     6121 gggagtgtct caaaatgaac tttttagttc atgaggcact cttttttata tgaaatcaaa
     6181 tatattttat catttcaaca ataaaaaaga agcttatctt tattttgaga cagtctctta
     6241 tttttatgta taccaaaacc atacctacga tttttattta taattaagtt tcaaatagaa
     6301 tgaacaagta caaccaattt tgaatataaa tatagtaaat gtgcttatat acgattttat
     6361 tgtctggttg attgatattg atagatagct ttctttattg aatcgaaggg agggggaatt
     6421 tgttatggat aactataaga aagtgaagca agtattgtgg ataattttat ttgctaattt
     6481 tgcagtagca ttattaaaaa tcattattgg taatcaaata aaaagctata gtatgacagc
     6541 agacggattt cattctttgt cagatggagc atctaatata gtagggctta taggtatttt
     6601 ctttgcatct aaaccaaaag ataaaaatca tccttatgga cataaaaagt ttgaaattat
     6661 aaccagttta tttataagtg gtatgttgtt tgttatagca attaagataa ttcttagtgc
     6721 agtgttaaga ataactaatc cagttgtacc tgctataact atagaaagtt taattgcttt
     6781 aatcattaca ctctttataa atatttttgt atgtatgtat gaatatagag ttgggactaa
     6841 actaaatagt tatgttttga tttcggattc attgcataca agaagtgata tatttgtgtc
     6901 tctaggtgta ttggttactt tagttggtgt taagttagga tttccagtaa taatagaatc
     6961 tatagttcct attattattt ctgcgttcat aatttattcg gcttatggaa tatttaggcc
     7021 atctattgga attttggtcg atagagtggc tgtagatgag gattatataa aagaaattgt
     7081 gtttgaattt aatgaggtaa gggatgttca caatattaga agcagaggta gcaaaagttc
     7141 tatttatatt gacatgcatg taatggtaga cccatttata agcgtagagc aatctcatga
     7201 tttaacacat aaaattgaga aacaaataca agaagaaatt aatgaaaatg ctcaagtaat
     7261 agtccatatt gaaccttttt atagttttta atttagctat aaaatacaag ttataaaaaa
     7321 atataattta taaaaaaaat attataccat aaataattta taaaaattat ttatggtata
     7381 atattttcta taaagaaatt aagtcttaat atatctaagg aggaatttac aatgagatac
     7441 gttgaatatg gaaagacagg gaaaatgatt tcagtagtcg gctatggagg attaaggttt
     7501 gatttagaaa aaagtaatca agaaaatgct gatttagtca aatatgcgta tgaaaaaggt
     7561 attaattact ttgatacagc accagggtat tgtgatgaca gaagtgaaga tatatttgga
     7621 ctagccttta gacaaatgat aaaggaaggt aagaccgatt tttatgtatc aactaaagaa
     7681 aagccaaaag catgtgatac atcagaaaaa gctatagagg ctgtaaaaaa atcaatagaa
     7741 cgattaggcg tatctaaaat aaatttttat catgtttgga atattagaaa gatggaacat
     7801 tacgaactag caatgaaacc tggaggtcaa tatgaaggat tattgaaatg taaagaagaa
     7861 ggattgatag accatatagt attttcatct catcaaccag gagatgaggt tatagatatt
     7921 ctaagtgaaa ataagtttga aggtgtaact atgggaatta atatattaaa ctttccatat
     7981 aggctaaaag gtgccaagta tgctgttgat aatggatatg gtgttgtggc aatgaatcca
     8041 ttaagtggtg gaactattcc aaagtataat aaagaacttt cttttctatc aaaagatggt
     8101 gaaagtacta tagaatcagc tttaagattc aacataggaa tttcacaaat aagtatatct
     8161 ctgatagggt ttaataaaaa acaagatata gatgatgcat gtaaaattgc agacgaaaat
     8221 agaatttatt cagatgagga tatacatgat attgagacta gattaaataa gaatatgaat
     8281 gaaatatgta caggttgtgg gtattgtaaa gtatgtccaa aaggtattaa taccccagca
     8341 tatatgcttt tttataatga aaagcagatg tttaaaaaga gtgatgaaga aatgacaaag
     8401 ttagtttatg gcttaggaca ttggaattat acaatgaata gtaaggcaaa agctaaagaa
     8461 tgtatatctt gtggaaaatg tgaagttgaa tgtactcagc atttacctat aatagataga
     8521 ttaaaagaaa ttaaaaaatg ggaagaagat ggagctaata gtgttaaagc ctaattaatt
     8581 ttatactata tttgaaattt gtatagtatt tagtaatgct ttaaaatata gttttaatat
     8641 ttaaggagac agattataat tttgtctcct taaattatgc tttaatttgt tatagtttaa
     8701 ttccataagc aacgttgtat atttttatat aaagtttttc aaatatttta cattaatatt
     8761 gcctacatta atattgctta caaattctca ttttatacaa cactaatcta tgttatataa
     8821 acaatacttg actaattata acatcaataa agtaaatatt gtggttgtaa ataaagagtt
     8881 attcaattac aataaaacaa gtaagaacat tatgaagatg gctattaaat ataaggaata
     8941 aattgtaggt gtcagcaaat cctatataat ga
//
LOCUS       DAEMAE010000151         8863 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEMAE010000151 DAEMAE010000000
VERSION     DAEMAE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.153"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(307..1047)
                     /locus_tag="KPH93_002701"
     CDS             complement(307..1047)
                     /locus_tag="KPH93_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG1689503.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(1068..1856)
                     /locus_tag="KPH93_002702"
     CDS             complement(1068..1856)
                     /locus_tag="KPH93_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG1689504.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(1944..2034)
                     /locus_tag="KPH93_002703"
     tRNA            complement(1944..2034)
                     /locus_tag="KPH93_002703"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1998..2000),aa:Ser,seq:gct)
     gene            2293..3057
                     /locus_tag="KPH93_002704"
     CDS             2293..3057
                     /locus_tag="KPH93_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG1689505.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFISTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(3337..4452)
                     /locus_tag="KPH93_002705"
     CDS             complement(3337..4452)
                     /locus_tag="KPH93_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG1689506.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGVIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKNEV"
     gene            complement(4493..6019)
                     /gene="glpK"
                     /locus_tag="KPH93_002706"
     CDS             complement(4493..6019)
                     /gene="glpK"
                     /locus_tag="KPH93_002706"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG1689507.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            6312..7409
                     /locus_tag="KPH93_002707"
     CDS             6312..7409
                     /locus_tag="KPH93_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG1689508.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIKNSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLEKESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSRLEIKKVI"
     gene            complement(7985..8497)
                     /locus_tag="KPH93_002708"
     CDS             complement(7985..8497)
                     /locus_tag="KPH93_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689509.1"
                     /translation="MKVKKFKSGIVMALTVIAIGTSLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKVNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT         2869 a         1561 c         1017 g         3416 t
ORIGIN      
        1 aatcttatct accttcttag attgcttatt agctttattt aatttttctt tatttaaatt
       61 ctcaactttt ttattatacc tttctcttgt tttatcttta tatttagtat ttttattttt
      121 atttcccatt catttcccat cctctataaa atttttttaa ttgagcttat atattattta
      181 aatattatat atttaagata ccatatattc ctacaaaaag gtaacttact atgtatctat
      241 ttttaatttt tgcataaaaa agccatctcc tttagaatta attctgtatt gagacagcta
      301 atttaactat ttatcctttg aaacattttc tacattatca tatttactat caactaaaac
      361 tatatcgact cttctatttt ttgacctacc ttcttcagat gaattttctg ctattggtct
      421 aaactcacca tatcctacag cagataattt atctggtgct atacctgcat tgtcaataag
      481 aagttgaaca acattggttg ctctcataac tgataaatcc caatttgact taaactctga
      541 attttttata gacatattgt ctgtatggcc ttctactctc atgtaactat tcatttcatt
      601 taacatctta cctatttgaa taatcttatc tctagaatta tcctttacaa ctgctttccc
      661 tgtgtcaaat agtattgtat ctttaaggct tattactaat cctctatctt gaactcttaa
      721 tgacactgaa ttagccatac cattttcatt taaatacttc tggatttctt caccaacttt
      781 tttaaactta gcattttgca aagtatctaa atcattactt cctggttcaa ctactggttc
      841 tattttgcta ttaccacctt caataactcc agaagaacca ccaaaagcag aatttagaga
      901 ttgagataat tgtttatatt tctccgcatc aacattactc atagaataca ttattacaaa
      961 gaaaatcata agtaatgtta tgaggtctga ataagttaaa agccatcgct cattgttttc
     1021 ttctttttca tcttcatcat acaacataaa tattccatcc ttttatatta ttcagctcta
     1081 ccaccaattt cttcaagttc aattagttct tttgaattta aaaatatttt taatttttct
     1141 gtcattaaat tagaattatc accctcaagt atggataata ttgcttctat caccaaaagt
     1201 ctttcactta tttcctgttg atttatagcc ttaagttttg atgctattgg taaccatatt
     1261 aaattggcag aaccaacacc atacaaagta gccaaaaatg caacagaaat ttgaggtcct
     1321 aatgcatctg gattacttga aagattgcct aaaatatgta ctaatcccat aactgtacct
     1381 attatcccca gtgtaggtgc atatccacct gctgaatcaa aaattgctat tccttcttta
     1441 tgtcgttttg atgttatctg tatctgtgat tccaatatat ttcttatagt aactgcatcc
     1501 accccatcta ccataagttc gagccccttt ctaataaaag ggtctaattc atcattctca
     1561 cttatttctt tgtcaatact aagaagcccc tctttcctag cctttgttgc aatttctttg
     1621 aaatagatta tattttctat gttattaggc tttttatttt taaaagctaa taacattatt
     1681 tttatagatt ttttaaacac agggaatggc gtcgaagctc caacagctcc tatagttcca
     1741 ccaaacacaa caagtgctga tgaaaataca aataatgctc caacatgtcc cccatcaata
     1801 acaaaagcaa ctaccagaga tgctatcccc actatgaaaa aaattaaagt cgtcatctat
     1861 tccctccttt ctttagttat acatatctaa tcatttatga cattataaaa agactatctt
     1921 taacaaaaat agcctttaaa aattggcgga gaaggaggga ttcgaaccct cgcaccggtt
     1981 acccagccta atcccttagc aggggatcct cttgagccac ttgagtactt ctccatatct
     2041 ctatactagt aaattatata ataaaatcat attttagtca attgttttat aaaatttgag
     2101 ttattttgaa ttattaaaca tctattttta cttttttgct taaatgtaag ttttaaatat
     2161 ggtaaaatta tcttgcataa cacttataat attatttgat aaatatacat acattaacaa
     2221 atatacctaa actctgactt aattgtaagt tatgccaaaa ctaaaataat actagtccaa
     2281 ggaggatatt ttatgttacc acaggaaaga tacgagaaga ttttaagtat tctcaaatca
     2341 gataatataa ttaaaataaa tgacatagtt caaatgtttg atatatcaat agaaactgca
     2401 cgaagagatt tagcccatat ggagagtatc ggtcttttaa aaagagttta tggtggtgct
     2461 ataccagtta tagcaagtag cgtagaactt aactatgtca aaagaaaaaa aataaactca
     2521 aacgataaaa aacagattgc tattaaatgt agtgagttta taagcacaaa tgatactatt
     2581 tttatggata ctggaactac tgtacttgaa gtttcaaaaa atttgaaaga taaaaagaat
     2641 ctaactgtaa taaccaactc aattttagtt gcaaatgaac ttatgaatac taatataaaa
     2701 gtatatgtgc ttggtggcta tcttagagaa actgaaggtt ctttttctgg tccgcttaca
     2761 ctgtcagcta ttgaacaatt taatgttgat aaagctattt taggtgctgg aggtatcact
     2821 cttgaaaatg gaatctctga ctatcactta gaagaagcac ttgttagaaa aaaaatgatt
     2881 gaaaggtcta gaaaatctat agtagttgtc gaacctccta agtttggtat aaattcattt
     2941 gcatcagtag ctcctataga acatatagat actattatta cctctgctag tatagaccat
     3001 aatataataa tagagttttc tcaaaaagga atacctttag tcatagctga taattagctt
     3061 gtatgattga tttttagttc cttgatgtaa taaattaaaa ttaaggagcc aaataattat
     3121 ttatttagct ccatttatgt ctaaactata ctttatattt taattatgaa ctactttcag
     3181 ttaacttatc atttttagtt atttgactgc cattttattt aatttataag ttttggttat
     3241 tcgattattt tttaattgtc tgtcttttta atttattttt agttatctaa actcctactt
     3301 taaaataatt taaatttgac ttaattatta tttattctag acttcatttt tcttcatttt
     3361 aatactttgt tcttcttcca aagttaaatc ttttgtcata agtatggcaa gtgcaattgc
     3421 caatactcct ccaaccaatt ttccaattac aacaggtgta atcatctctg gaactactcc
     3481 tgctgtaaat cctaaatggt ctcccaaagc tgctgtagca caaaccagcc aagcagtatt
     3541 tactactttc ccctttggac tcatatcttt catcatttta tatactggaa tactattggc
     3601 taaagtaaaa actaatcctg ctgcactagt ggcatccata cctaatttcc cacccactat
     3661 tgtaagtggt ttattaagaa ctttaactaa taaatgtaat attggaaaag tacctaataa
     3721 cactattgca atacttccaa ttacttgaat accttctctt ataggtgtca ttccttttat
     3781 aagtactata cctgttattt cttgaaatgc tgctgctccc aaacctatcg ttatcaatat
     3841 agatataaat tttccaaaaa ctaatgaccc atttatcata agtgtagaat taaattttaa
     3901 tcctactgcc aatattactg ccaatattat cactggtatc atatttataa ttaccattga
     3961 taagttaaat cctgctatta atcctcctat aacacctcca aaaggtattg ttatcaggcc
     4021 tatcagtaac ccttttgcaa agaatggtct gtcttcatat tctataagac ctagtcctac
     4081 tggaattgaa aatactaatg tacatccaag catggatgac acgaccaatc ctgcaagcat
     4141 tcctgcctct ttattgtgtg caagttccat agccaatgga taccctccca tatcattcgc
     4201 taaaatagtt gcaaacatag atgggtcagc tccacagaga ttgaagattg gtattattat
     4261 aggacctagt acatctgaaa ttactggagc taaacaaact ataccaacca ttcctaaagc
     4321 caatggaccc attgaattaa acccttcttc aaactgctct ccaagtccaa atttatttcc
     4381 aattattcta tctaacgctc ctaaaagcac tcccacagcc attatgtata atataatttt
     4441 atcaaagggc atttttaatt cctcctatac atgtaaaata ctctattgta acttaattag
     4501 cacacacctt tttggtatcg ttttcccaat tcatagctct ttttactgct tgattccatc
     4561 caagtaatat ttcttctctt ttttgattag tcatatttgg aatatatttt ttgcttattg
     4621 taaattcact tctaatttca tcaatactgt tccagacacc acaggaaaga cctgccatat
     4681 atcctactcc aagtactgtc gtctcgggat tttctgcaat ttcaattggt atacctaata
     4741 tgtcggattg aaattgcatt gtaaagttat ttttagtaat tccaccatct actctcaaca
     4801 tttttatttt tttccctgag gctaagttca tagaatcaac aacatccttg acttgaagtg
     4861 caacagactc aagagtcgct cttactatat gctctttttt agtacttcct gttatcccaa
     4921 ccattgtacc tctagcatat tggtcccaat aaggtgctgc tatacctgca aatgctggta
     4981 caaaatatac acctccagta cttgatatgg aatttgccat gtcatcagtt tctgaataat
     5041 tttttataac tccaattcca tctctaagcc attgtattgc agcacctgct atatacactc
     5101 caccatcata agcatatgta actttatctc ctattttcca agcaacagtt gtcaaaatac
     5161 cattacctgc tatatttagt tcctcaccta tattcatata tagaaaacat cctgtaccat
     5221 aagtattttt aaccattcct ggttcaaaac atccattgcc aaataaagct gctggttggt
     5281 ctaccattga agcagtaatt tttatagaaa ccccacaaaa tgcctcctta tctgtagttc
     5341 caaaatcacc actcgtaggc attatatctg ctaaaatact tctaggcact cctataatgt
     5401 caagcaactc tctatcccaa tccatatctc ttatattaaa aagcattgtt cttgctgctg
     5461 tagatacatc agttaaaaaa gattttcctc ctgtcatttt ccatattaac caactatcta
     5521 atgttcctgc aagtacttct ccatttttaa ttttttcttt tacccctttt acattatcta
     5581 ttacccattt aatttttgta cctgagaaat atgggtctat aattaaccca gtcttatcta
     5641 ttatcttatt ttctaaacct ggtatattct ttaaattttc cacataatca tacgttctcc
     5701 tgcactgcca tacgatagca ttgtaaagtg gtttacctgt atttttatcc cataacatta
     5761 ctgtttcacc ttgattatct ataccaattc cttttatatc actttcattg attccatttt
     5821 caaaagcctg ttgtaataca ttattactca cagtttttgt attattccaa atttctatag
     5881 ggtcatgttc gacatatcca gattgtggat atatttgagt atgttccata taatcactaa
     5941 aaacaaactc tgatttttcg tttatcaata ctgctcttgt tcctgttgtt ccttggtcaa
     6001 ttgctaatac atatttcact ttaaatgtcc ccctttattt tcagaattaa taaattcttt
     6061 tttatttttt gaatatataa tctattcaag ttatttatat tttatatcat catgtggatt
     6121 tatgcaatat tcattacaat attttttgtt atttttaatt atcgtgatta aaaaagtatt
     6181 tttattgaaa tattattgca atatttatta ttttattcta taatctagtt aaaataacac
     6241 aaaatatttt aatatttaca catagtcagt cattcaaaaa tttaaattta actttttaag
     6301 gaggttttac aatgaaaaaa acgtatctat tcagtcctgg gccagtaatg gttacagata
     6361 aagtcagaaa ttcacttctt cactatgata tatgccatag aggaaatgag tttatggatt
     6421 tattcaaaga tacacaacaa aaaatcaata aactatataa tgcaacttct aattactact
     6481 cattaattgt atctggctct ggaacatctg taaatgaatg tgtactatct tctatttttg
     6541 acaaagaaga tgcagttctg ctagttagta atggtgtttt tggagaaagg ctagaagaaa
     6601 tattatctac ttataatgta aaaacatata agcctaattt tgaatgggca gaatacccgg
     6661 acctagacat tttagaaaaa tacataatta aaaattctga tataaaagta attgctatgg
     6721 tgttccatga aactagttca ggaatgataa atccagttcc tgaactagga aaacttgcta
     6781 agaagtataa caaaatattt tttgtagatg caataagtgc atcagcaggt gaatatattg
     6841 atgtggaaga gtttaatata gatataataa caggtgttgg tggtaaagct cttggagcat
     6901 ttccaggttc tgcatacctt tgtgctaaag agagtatttt acaaaatatt aaagaaaatc
     6961 aatgtaagaa tgtgtatctt aatctttata aacactataa agttgctaaa tcatcttctc
     7021 aaacaccaaa tacacctaat gttactttaa tatttgccct taatgaagct ttaactgagt
     7081 ttttggaaga tgacagtaaa atagaaagat acaaagaatg ttctgctatt ttaaggaatg
     7141 gcatggaaga attgggtcta acatttttac ttccagataa gtatatgtct aatacagtaa
     7201 catctgtatt tctacctaaa gaaatcaatg taaatgagtt tatattaggg ctagaaaagg
     7261 aaagtggcta tgttgtctat ccaggaaaag gcaaattcct agatgctaat atattccaag
     7321 ttgcaaatat gggagaaata tatccagaag attgttataa attcttagat atcttaaaat
     7381 caagattgga gataaaaaaa gttatataac agtttgtttg taaaatccta caatatatca
     7441 gtataaatta aatagtctga ttttttaaaa ttttcctata atatcctata ataaattata
     7501 ttttatactt ttgtataagg gtgaactata atatgtaaag ttcaccccta tttcaaatct
     7561 ttttccttta gtataatctt tttcttttaa tttattttat atttttgata caattattct
     7621 tacatttaat tgttttttgt tttgaaacaa tcgtttttta tttaatcatt ttatattctt
     7681 gatacaatca ttcttacatt taattatttt ttatttttga cacaatcatt cttacattct
     7741 attattgttt gtttttgacg caactttttt tattcagtca tttgatattt tcaacacaat
     7801 cattcttaca ttttattatt gtttgttttt gacgcaatcg ttttttattc agtcatttac
     7861 tattttcgac acaatcattc ttacattcta ttattgtttg tttttgatac aatcgttttt
     7921 tattcagtca tttactattc tcaacacaat taccttttca ttcaatcaaa gtaatcttta
     7981 agcattattt ttcaagctca tcaaagtaat tatatacgat aacctcacct gtctctttat
     8041 ctgtagtctt tataatgccc ttagctccat ttacctcttc ttttgtaaat tcatcatcta
     8101 acacaacttt gaatttatct tcctttccat aaccttgttg aaattcatct ctcaaaattg
     8161 catttttttc tgcttttggc ttcaactcaa attcaatccc agatttcatt tcatcaaaat
     8221 aatttaatac tacaacctcg ttattcactt tattagttgt ctttataatc cctttagctc
     8281 catttacttc ttcttttgta aattcatcat ccaatgttat attaaatttg tcttcttttc
     8341 catattcctc ttgaaaatcc ctcttcattt cattgtattt agtttcaaac tccttactct
     8401 tgtttaattc catagctgat acatttgtca atgatgttcc aattgctatt actgttaatg
     8461 ccattactat accactttta aattttttta ctttcatatc ttacctccta aattaaatgt
     8521 tctcaatgaa attatataaa gaaaatccta agttttacag tttcattctt gtaactaaaa
     8581 ttactattat gtaaccaaac tttttgtttc aactattcct taaaagttgc ttttttgagg
     8641 aatagttgga gttaaataat gagattttat ctatatttat agatttaaaa tcaaatttat
     8701 ccatataaat aaatacttat ttttgcaaaa taaaaatttt taattactct aatccttctt
     8761 atttacttaa aatcctatga taagaaattt aagagtcttt accttcttta ataataattt
     8821 atttactttt aatatatcga gtatatcaaa attattttac tga
//
LOCUS       DAEMAE010000152         8862 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEMAE010000152 DAEMAE010000000
VERSION     DAEMAE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.154"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(260..823)
                     /locus_tag="KPH93_002709"
     CDS             complement(260..823)
                     /locus_tag="KPH93_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689510.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(983..1696)
                     /locus_tag="KPH93_002710"
     CDS             complement(983..1696)
                     /locus_tag="KPH93_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG1689511.1"
                     /translation="MGKIAKILLSLIVIVLVLASIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPNLKDGNLVVSLENVKLGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPVTLKNISIKRGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1988..3652
                     /locus_tag="KPH93_002711"
     CDS             1988..3652
                     /locus_tag="KPH93_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG1689512.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3759..4658)
                     /locus_tag="KPH93_002712"
     CDS             complement(3759..4658)
                     /locus_tag="KPH93_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG1689513.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDNSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4685..5323)
                     /locus_tag="KPH93_002713"
     CDS             complement(4685..5323)
                     /locus_tag="KPH93_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG1689514.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKGIKPEDMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5664..6662)
                     /locus_tag="KPH93_002714"
     CDS             complement(5664..6662)
                     /locus_tag="KPH93_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG1689515.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNIFYIATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFITSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNVGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6760..7335)
                     /locus_tag="KPH93_002715"
     CDS             complement(6760..7335)
                     /locus_tag="KPH93_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689516.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKINKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            7651..7914
                     /locus_tag="KPH93_002716"
     CDS             7651..7914
                     /locus_tag="KPH93_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689517.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(8351..8728)
                     /locus_tag="KPH93_002717"
     CDS             complement(8351..8728)
                     /locus_tag="KPH93_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1689518.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         3122 a         1355 c          930 g         3455 t
ORIGIN      
        1 tatatgtgta tgtataaata ctttttatac actatttgtg ctatccatct cacacctaaa
       61 ggagtgggat ttctgtacta ttctgtaata tatatatcta aaaatattat aaaaatacaa
      121 atagtatcat aaatttaaaa actgaagaaa gtattctaac taagatttac aaattcttaa
      181 tcctatcgaa gttaaactac tatcttcagc ctaattatct agcaatattt ttttatatga
      241 aaaactctaa atcaagtttt taaatgtttg cgatttttga tacataagaa tgttgtctag
      301 aattatattc tgataataaa ccttcatact ctaaggttaa gccagataaa ttataaaaat
      361 catttggcgt tacttcaaat gcttgtttat ttaaaatgtc tatctcactt ttttctaaca
      421 tgtcagcaac aaaatttgaa catacatatt tatattcaga ttgtctctga atattcatta
      481 gtaaatatat taatgccatt gtatcgtaat agtaatcttc tctataatca ctaattaatt
      541 ttatattttt atatagattt tcatattgta aattatctac ttctaacgaa tatactctac
      601 acacagtgtt ttttcttata gcatataatc cttgatttat attttcttca acaaatcctc
      661 caataaatgg attccttgga ttcagtcttc caaaagaata cattggtttt agattttcat
      721 ttaaggatat tgacacatgt gtatacagtt ttttacttat attccttatt aagtaagata
      781 atatagttcc tgtatatgtt gttacaatat agactttatt catcatttcc ccccaatcta
      841 gtaactaaat aatattatac acttacaatt taatatcata agatattcta acataataaa
      901 tgtggtttta caaattttga ttttaaggta ctttaatatt atataaaata taataaaaat
      961 ataaatataa gttttctata ttttaaccaa cagatgtttt cttcaaatta atcagatgat
     1021 ttagtatatc ttgaaccttt ccagagtcct ttaaatatcc taatgcccca tttatttgtg
     1081 attttaaatc tatttctact tccatatcca aatcaacatt tcctcttttt atagatatat
     1141 ttttaagtgt tactgggtaa agataacttt tttccaaagt aatcttattt ccattaacct
     1201 caaatggaac ttttttattg tagttactta atattttttc taacatttta tcacttagct
     1261 taaattttcc aagtttcaca ttctcaagac ttacaactaa gtttccatcc tttaaattag
     1321 gtctcagttc aagttcatat tgactattta ccaaaccaaa taccttatat ggtcctgcta
     1381 cctttatttt accatttttc atttcaacaa aattattttc aagctcatta atgccgtgct
     1441 tctgcatcaa tgtatatatt aaatttttaa actcttcctc tgaaacagat atttttccaa
     1501 ccattctaag tggatttttt actacttcca tagaatttat atatgagcct tttgataaat
     1561 attcttcatc tatgatttta ttattttgtg aactaacatc atatctttct tttggtgtca
     1621 agatatatac aacaatactt gcaagtacta aaacaataac aattaaactt aacaaaatct
     1681 ttgcaatctt tcccataatc gtctcctttg caaatctatc agcagattta aaataaattt
     1741 agataactaa cttcacataa tattcttaac aatatacata ctataataca gtcttttaaa
     1801 aattaattca accaatctca ttttttgtaa ataaactgta aaaacatgtt tttagtataa
     1861 aagttaatat tttgcataat actcttaatt acacctttat ttttttctaa tcttggatta
     1921 aaattaatat tatgcaatac agtgtagtgt tctgtattat aatcaaatta aataggaggt
     1981 atagaatatg tcaaatgaaa cgaactcatc taactttata aaaaatatta ttataaacga
     2041 ccttgaaaca ggaaaacatg acagtatcat aactcgtttt ccacctgagc caaacggata
     2101 tttacatatt ggtcatgcaa aaagtatatg tcttaatttt ggattagcta aggagtttaa
     2161 tggaaaagct aatttaagat ttgatgatac aaacccatta aaagaggatg ttgaatatgt
     2221 agaatccata aaagaagatg tcaagtggtt agggtttgat tggaacgagt taaattttgc
     2281 atctaactac tttgatgaaa tgtataagag agctttaata ctaataaaaa aaggtaaagc
     2341 ttatgtatgt gatttgacac aagaggaaat gagagaatat cgtggtactc taactgaacc
     2401 aggaaaagaa agtcctcata gaaacagaac tattgaagaa aaccttgatt tatttgaaag
     2461 aatgaaaaat ggtgaattta aagatggtga aaaaacatta agagccaaaa tagatatgtc
     2521 ttctccaaat ataaacctta gagacccaat tatatataga atatctcatt caacacacca
     2581 caatacagga gataaatggt gcatatatcc tatgtatgct tttgcccatc caattgaaga
     2641 tgcaattgaa ggaataactc actctatatg tactttagag tttgaagacc aaagacctct
     2701 ttacgattgg tttgttaaag aatgtgagat ggaaaatata cctagacaaa tagaatttgc
     2761 tagacttaat ataaataata ctgtcatgag taaaaggaaa ttaaaacaac ttgtagatga
     2821 gggtattgtt gatggttggg atgaccctcg tgttcctaca atatccggaa ttagacgtaa
     2881 gggatatact gcagaagcat tgcgcaattt ctgtagtgaa ataggggtat caaaagttaa
     2941 ctctacagta gatagtcaaa tgcttgatta tttcctaaga gaaaacttac aaccaaaggc
     3001 tccccttact atgggagttt taagaccttt aaaacttatt ataaccaatt acccagaaga
     3061 taagattgag atgcttgaaa tagagaataa tgctaaagat gaatctcaag gaaagagatt
     3121 agttcctttc tctagagaat tgtatataga acaagatgat tttatggaag aacctgttaa
     3181 gaaatatttt agatttttcc caggaaatga agtccgttta aaaggtgctt attttgtaaa
     3241 atgtactgat gtaattaaag atgaaaatgg aaatgtagtt gaaatacatg gtacttatga
     3301 ccctgaaact aagagtggct ctggctttac aggacgtaag gtaaaatcta caatacattg
     3361 ggttgatgct aaatcagcta taccatgtga atttagatta tttgaaccat taattcttga
     3421 tgatatacct gaaaatgaag gtaaacattt cttagaacaa ataaatccta attcattgga
     3481 aatattgcaa ggttttgttg aacctacaca gataaaagat gctaaacctt ttgataaatt
     3541 ccaatttgtc agaaatggat tctttagtat agataataaa tatacaactg atgaaaaatt
     3601 tgtatttaat agaatagttc ctctaaaaag ttcttttaaa ccaggtaaat aatcttacaa
     3661 tcaaaaaatg aagacttaaa atataaatta cttctactat aaataaactt agttagaagt
     3721 aattttttat ttgagtcttc attatttact ttaaatgatt aacttttttc actacagatt
     3781 cccataactt ctattccatc ttttatgcat tctatgttaa gaggaaaatc tggtcctttt
     3841 tctccgtcaa tatctgttat aagtgaatct tcacattcta tatgcacctt tttagtttta
     3901 aagtatagta tttcatttcc attatattga tctaaataat cacctctaag cacacttatc
     3961 aaaactggta ttgattttgg tattggcatt cctttaaaaa taattacatc caaatatcca
     4021 tcatctattt cagccttata tgctagattt atatttcctg ctgttttgcc attaaaaatc
     4081 aacataagat acatatctcc atcataatac atttcctcag aagatacttt tatcttaaat
     4141 ttcctcatat acaatgcttc ttctattccc tttatataat aagaaattct tccaaatgaa
     4201 ttttttaagt ctgtgtttat tctttgtgat acatccgtaa acataccagc acttgcaaca
     4261 tttataaagt acttattatt gactttccct atgtctattt tctttggttt tgaatttatg
     4321 atttcattta tagcttcttt tatatcaaat gaaatttgaa gagcatttga aaaatcattt
     4381 gcagttcctg taggcaaaat tccaattgga atttttatgt ctaattcttt cattgcattt
     4441 actactaaat ctacagtacc atcaccacca gctatcaata tgtgttcata agaattatca
     4501 acttccttaa atgcattctt tacaggttct ttaatagtta atctataggg tatcaataaa
     4561 taattttttt cttgatataa ttctatgata gtgtctaaat ttgataatat ctttttttca
     4621 cctgaattag gattgtatat aagttttact tttttcatta tttctcctta ctcattttat
     4681 tcttttagta tccaaaatct tcatttaaaa aaatcacatg tattctttct ggtgttaatt
     4741 gtctttttat aagtgtataa ctacagcata ttcttcctgg acttgaacag tctatacata
     4801 taccagtagt cttacatcca gtatttgtac ttaatctttt acaattggct ggagcagcta
     4861 ttgtttttgt tctttttata gcttcatcta catctttaac tattttattt actcctgcta
     4921 taactattac tttatcaggt ccatatatca tagcagcaac tctattccca ttcccatcta
     4981 aattgtaaag ttctccatcc tctgttatcg cattagtact tgtaaaataa gcatctgcag
     5041 aaaatgactt tctgtatatt tccttcatat cttctggttt gatacctttt gcatatctat
     5101 caagaaaatt atatttttct cttataaaat caatcactcc tgtttcaaat aaggtcatag
     5161 aacctccaca tcctactaga gaacccactt taactatttc ttcaattttt ttaagaagct
     5221 cttctttgtt acttactaag taccctttca tattattgtt ttccagagat tttattgttt
     5281 tttctattct tttttcatta agccaattta aattttgatc cattttaact tcccccttaa
     5341 tttttaatat gtacttaatt atttataaac tttattttct ttataagttt attttagtac
     5401 atctaaaaaa aaatacaata taaaacttaa atagttttcc ataatttttt ccaaaataac
     5461 ttactcaatc aaaaaaggaa agtagatgta ctattctact ttccctatat tgtatgtagt
     5521 attatatatt gcctaaaatc tatatattaa ttatatgttt catattatag aatatcacag
     5581 ttaattaaaa tgtaatacta tattcttttt aaacatataa attgtattat taaatttaat
     5641 atatccaact tcaataaatc ttattaattt gatgaagaag atgctatagc tgcacttgtt
     5701 gctgccatta cacatgcaat ttgaatagca acaacaatat ttaaaaatat attattagtt
     5761 atagaaccta cattattctc atttcgtatc aaatctgcat aaattgaagc tacaatagca
     5821 gaactaatca ttactctatt actttttcct aaagaccaat ttccaaagcc tttttcttct
     5881 ttaagtttat ttgaaacatt ttttatctct tctattattt catcttctct ataatctaat
     5941 aatgaaattg ctcctattat tggatatcca taataattca ttttacaatc attttcttta
     6001 aatgcttttt taagttcaat agtcttctta catttaagca tagttctatc atcactaaat
     6061 aaaagaattt gggataatgc ttgtaaatca tttcctttat aaaaaccatt ttcatttaga
     6121 aattcataac atttttctat gtattctaaa tcttcatcta aattttttga atgaattgca
     6181 ataagtgcta tattacaata gtcatcacta gaagttataa atggatgttt tttcctcatg
     6241 taatcatata ttattttcat ttttgatata catgtaaggt aatccatttt ctctctattt
     6301 tcataaacta ttgatgctgt aagtggtaaa tatgtatcgc cccaaaactt ttcactttta
     6361 agttctttgt atatatctaa aatcatctta aaactacttt ctggatttga ttcaaatgaa
     6421 agtagtgttg ctatataaaa tatattatat cctctaaaat tagagaataa tccagtattt
     6481 tttttaataa gctctattcg ttcttctact aatctaggac ttattctctt gttcttaagg
     6541 gtaaatgcaa gtgcacaact atgttgtatc aatcccattt gccaagaacc tttaacttca
     6601 tttaatgctt tgtaattttc tattaataag tttacttttc catttataaa agattgttcc
     6661 atattgccct cctagtaata tatctcattg tattaatagt tttatattta ttatatatat
     6721 attaaatact acaatcaata catattactt caaaggacat cattcttcaa caataatgta
     6781 gccttttttg taatgtcttc ccatattatt gactctatca tagtactctg attcactttc
     6841 attactccat ttgggcacaa atacatctat tgcattagaa ttactaaaat acttactaga
     6901 ccccctatcc ataacagttt ttaattttcc atctacaata atcttagttc caaaactcaa
     6961 gtgattgttt gctacaaaac catcttctaa atcatctcct gtagcagtta tcgctccata
     7021 tccattttct tgattaagac ttgtgtaata actaacttca aaagttatag gtactaactt
     7081 atttatttta atacccaact gattttgtag tttctccttt tgtaccttca acttatactc
     7141 atcactattt ttatagtctt ccataccttg attatacttg gaattaaagc ttttacctga
     7201 ctttactata gtatctatat ttactataga ttctttacaa tgatctgcat ctatttttgt
     7261 ttctccattt accaataatg gagttaaaca tacactacat acaacacatg taatcaatgc
     7321 ttctttaagc ttcataaatt ccccccttat tattttgata tattagcata tttaatatta
     7381 tatgtctaca tttagattta aataccacac ccatttgctt taagaagttg tcttatattt
     7441 aattcacctt aaacacttat aaatcctcta tcttagaatc atatagaata aagcttttat
     7501 cgttaaatat tttttattaa tcttttaaaa tctaaaacta ctgtatttaa ttttctcttt
     7561 tttagaattt aagaattaaa attctataat tttaaaacat attgaattta tttttttgag
     7621 atgatataat tttcttaaga ggtggttgat atgataaaaa atacaaaaca aattacagta
     7681 gaagttaatg aaattttatt acaagaaata tctaagataa ctaatgacga aaataatatt
     7741 ttgacagatt tgataaatga atcaattgta aattatattc ttgaaaacaa aaaacataac
     7801 attgaaaata aagatttaga agaagtgatt aaattttaca aaaaagaaaa tactgaaaaa
     7861 cctaaaagtg cagcaagagc tatagctgaa gcaaaaactc ataccctgta ttaatcaaat
     7921 aaaaaagttc ctaaaaacta ggaacttttt tattttaata attattaata tatctcaata
     7981 tttatctcaa atttatcaat ataagcatgt ttaatacaat ctattgcata cttatcttac
     8041 cctagaaata atcaaaacaa aaataaaatt atgcccttta tatctaaaac aattaattta
     8101 catgattaat atacaaaaat tctgactttt aaattcgtat acaatatcaa actaaaatac
     8161 tattaataac aaacccaata taaattaaat tcaaacaggt ttgattttac atcaaactta
     8221 ttgtattaat attaaataac aagacaaaaa tatttataaa aatcacaaat tttacataaa
     8281 actttatatt tgattcattt aactaattat tgtaaaaact atttatttta tgataattaa
     8341 ctacaccaaa ttattcatct tttttaatat ctttaaccca tttttcaaca gagttcaatt
     8401 cttcttcatt tacccccttt ttgaacaaag atttcataaa actctcaata gaacttccat
     8461 gcatattatc taaaaaagtt tttgtttcaa aatgtaaata ctcttcttct ccaataagaa
     8521 ctgtataatg tgtatgtcta tcaatctttc ttgcacttaa gaattgctta tttactaatc
     8581 ttgataaaag tgttagtgtt gtagtttgct tccaattata tagtttttcc atagcttcaa
     8641 tgacatcttt tgatgctact gtaaaatctg aactccaaat aaatttcatg acttttaatt
     8701 ctgctggtgg tatcttagta atagtcatat gattttacac tcctctcaaa cttctacata
     8761 attaaatact atattaatag acttttcgag tcaaaggttt tatttaaaat atctcatcca
     8821 aatttattaa tacaaaaaat ttatattttt attcaaaatt aa
//
LOCUS       DAEMAE010000153         8857 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEMAE010000153 DAEMAE010000000
VERSION     DAEMAE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.155"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            251..1609
                     /locus_tag="KPH93_002718"
     CDS             251..1609
                     /locus_tag="KPH93_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1689519.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDNEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAMSIAIARIIGCIIIIRAL
                     FKGNEFISMSKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFVV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            1882..2262
                     /locus_tag="KPH93_002719"
     CDS             1882..2262
                     /locus_tag="KPH93_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1689520.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            2387..2587
                     /locus_tag="KPH93_002720"
     CDS             2387..2587
                     /locus_tag="KPH93_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689521.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            2783..3511
                     /locus_tag="KPH93_002721"
     CDS             2783..3511
                     /locus_tag="KPH93_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1689522.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            3533..4450
                     /locus_tag="KPH93_002722"
     CDS             3533..4450
                     /locus_tag="KPH93_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG1689523.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            4592..5776
                     /locus_tag="KPH93_002723"
     CDS             4592..5776
                     /locus_tag="KPH93_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1689524.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            5883..6071
                     /locus_tag="KPH93_002724"
     CDS             5883..6071
                     /locus_tag="KPH93_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG1689525.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            6221..6871
                     /locus_tag="KPH93_002725"
     CDS             6221..6871
                     /locus_tag="KPH93_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG1689526.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            6974..7912
                     /locus_tag="KPH93_002726"
     CDS             6974..7912
                     /locus_tag="KPH93_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG1689527.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            7938..8789
                     /locus_tag="KPH93_002727"
     CDS             7938..8789
                     /locus_tag="KPH93_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1689528.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         3577 a          845 c         1600 g         2835 t
ORIGIN      
        1 ttttaataat aactgaaaat atttatatta aattaataaa tattttaaat gtggtatcca
       61 taaaaaataa taaacataag ataattataa agatatactg tgttcaagtg atattaaaca
      121 agatttatat tatttgaaag cagtatatta taatgcgaac aatacaattc taaactttgc
      181 attgtcaatt tacagttcga taaactaatt aaaaattaaa tttatataaa gagggaaaag
      241 aggaataggg atgtttataa gaaaaaatgt tataaagtta gcaattccta taatgataga
      301 gcagacgttt gtcatgcttt taggagtatt taatacaatg atggcaggtc atattgggga
      361 agaagctgta tctgcagttg gaatggttga ttcaataaac aatatattta tatctgtatt
      421 tgctgcatta tctgttggag ctactgttgt tgtagctcaa caaataggaa aaaaagataa
      481 tgaaaaggtg aatgaaacta ttaagcaggc tctagtgtct ggagtaatat tatcctttac
      541 aataacaata ttgatgtggg tttttagaac tggtgtaatt aatttatttt atggttcagc
      601 agaagaattg gtaaaatcaa atgcaaaatt atacatagag tttacattat ttacgtatcc
      661 atttattgcg acacaacaaa tagcaaatgg tattttaaga gggtgtggag atgtaaagaa
      721 acctatgtat gtgactatgt ttatgaatat agtaaatgta acattaggat atatacttat
      781 atatggtatt gatttaaatc ctctaggaat aaatttgcaa acaccatcct atggcataac
      841 aggtgcagct atgtctattg ctatagctag aataattgga tgtataataa ttataagagc
      901 tttatttaaa ggaaacgaat ttatatctat gagtaaaata tttccattta agattgatgt
      961 tgaaactcaa aagtctatat ttaatatagg gataccagct ggagttgaac aactactttt
     1021 taatgcaggt aaattgattg ttcaagtatt tatagttact atgggaacat cagcaattgc
     1081 atccaatgca ataggaatgt caattgcatc cataataaat gtaattggga atgctttagc
     1141 tttgtctgct acaaccttgg tgggacagta tataggacgt ggagatatta aaggagctaa
     1201 gagtacttta ttatatttaa ctaaatttgc aacaatttgt ttatttgtag taggagctat
     1261 atttatacca attgcaccct ggatttcaag tatgtatact caaaatgcta gtgtaattga
     1321 catctcaaca caattaattc gaagtgattg tttagctatg gtaatatggc ctatatcatt
     1381 tgtattatca gcagggctaa agggagctgg tgatactaga tatacaatga tgactgcagt
     1441 aataggtatg tggattttta gaatttttac aggttacgta ttgggggttg tattagaatt
     1501 tggagtgtta ggaatttgga taggtatgta tactgattgg cttgttagag ggactttata
     1561 ttgtttaaga ttaagaggaa caaagtggct aaaacacaaa gttgcataga atatatgaaa
     1621 tcacttttag atggataact ctatttagaa gtgatttttt atgtagttat tatcataatt
     1681 ataagtatga tatataaggt gattaaatta cataaataaa gcctataata gtatggatgc
     1741 tataaattat atattgagca gaattacata atttggcgat aaatattaga aatatatatc
     1801 tatagaaaat aatatatatg ggtatattat taaatataaa aaatattagg tatttattta
     1861 ataataaagg tgggtattgt tatgataaat aaaataggaa aaataactct ctatgtaaat
     1921 aatcaagagg atgcaaagtt gttttggaca gaaaaattga actttgtagt aacttatgaa
     1981 aaggaaatgg gtccaaatat gaaatgggtg gaagtagcac caagtaaaaa tgaatttaca
     2041 acatttgtgt tgtatgataa aaatctaatg atgagacaaa acaaagagat aaatgtatct
     2101 catccatcaa taattttaag tacaagagat attaatggaa cttatgaaca aatggcaaat
     2161 aacgaggttg aggttgggga attaatggag atgccatatg gaaggatgtt ttcttttaaa
     2221 gaccaagatg gaaatgatta tttggtcaga gaggataaat aattttacaa agtattaaat
     2281 ttactcaata tctgatataa ttagagacaa taattattat aaactagtct aggattagaa
     2341 ttatataatt aaaaattaag tgtaaatttt gagtagaggt gataaaatgt ttaagaaaat
     2401 ggcagtacta aaagatatag caactaaaat aggtcgtaaa aaagcgtatg aactattaga
     2461 aatggttgaa ggtaatgatg cctttgtagc tgaggtgaag ataaaaaaga atggaataga
     2521 atctaaaaaa gaagaaatta tgttaaaaga taatcaaaaa ataatattag agtatataga
     2581 aggctaattt ttaaatacat ataagttgat atactaaatt tctgagatga gtttacataa
     2641 aactcatctt ttttactttt caataacttt gttttattat ttaatataca ttttatctgc
     2701 ttagagttgc tgatatataa aaattactga tataataaaa gaatggatat taatgtaata
     2761 ttttatagaa aagaggggta ttatgcttga aataaaaaat ctttccttta atgttgagtc
     2821 taacaatgag gaactaggaa taataaatga tgttagcctt tcatttgaga ggggaaaact
     2881 tattgttatc acagggccaa atggtggagg aaaatctaca attgctaagc ttattatggg
     2941 aattgaaaaa gctacgagtg gtcagataat tttagatgga gaagatataa caaatcttag
     3001 tattacagaa agagcaaaaa aaggaatagg atatgcattt caacaaccac caagaataaa
     3061 aggtatgaca gttgaaaatt tacttacact tgctcatgga aaaccattgt ctactgatgt
     3121 atgttgccaa tatcttacag atgttggatt gtgttcaaaa gactatttaa atcgtgaagt
     3181 tgataactct ctttctggag gggaaatgaa aagaattgag atagcaactc tttttgctag
     3241 agatttaaag gtttctatat ttgatgaacc tgaagcaggg atagaccttt ggagctttgg
     3301 gaaactcaat gaaagtttta agaaaataca tgaagagtct aaccagacta taatcataat
     3361 ttctcaccaa gaaagaatat tagagttagc tgatgaaata attgttctac aagatggttc
     3421 agtaaaaagc catggaacaa aagaggctat attacctgaa atcatgtgtc aagttaattc
     3481 aagttgtgaa ttaatgaaag acatgaatta attagaaagg aaggcaaatt aaatgaacaa
     3541 aatagattta aatcttttag aaaaagttgc tgaacttcgt ggaactccat taggagcata
     3601 caatatacgt aaaaatgggc aaggagtaga gagacattca actgaaaatg taactataat
     3661 tcctaaaact gataaacctg gaatagatat tatagtaaag ccaaatacaa aaggagaaaa
     3721 tgtacatata ccagtaatag taacagagac tggtgtacag gatatggtat ataatgattt
     3781 tgaaattgga gaaaatgcag atgttgttat aattgctggg tgtggtatac ataactgtgg
     3841 ttgtgatgat agtgaacata atggtataca tagatttttc ttacgtaaag gagctcgcct
     3901 aaaatatgta gaaaaacatt atggtgaagg tgatggagaa ggaaagagaa ttttaaatcc
     3961 agttactatt gtcaatatgg atgaggattc atattgtgaa atggaaacaa ttcaaattaa
     4021 gggtgtagat tcaactaaaa gagaaatgga tgcaaaccta ggtaaaggag ctaaaatagt
     4081 tgtatttgag aaattattaa ctcatggtaa tcaagttgca gaatctaata tgaaggttat
     4141 cttagagggg gaaaactctt ctgctcaagt agtttctcgt acagttgggc aagataattc
     4201 taagcaaaag tttaatcctt gtgttattgg ggattccctt tgttctgcac atgttcaatg
     4261 tgattcaata attatgggaa attctcaaat tagtgctata cctgaaattg ctgcaaacca
     4321 tccagatgct cttttagtac atgaagcagc tattggaaag attgcaggag accaaatact
     4381 aaagatgatg acattaggtc ttactgaaga agaagccgaa caacaaatac ttaattgctt
     4441 cttaaagtaa attaaagatt ttatttaatt taatataatt aatatagtat aatatagtga
     4501 ataagtctaa aatattttta tttttaggct tattttcttt atgtaagaaa agctattaga
     4561 gaaattatat tgtaattagg atgtggaata aatgaaaaat ttacttataa gaaatgggac
     4621 aataatatct ccaacagatg gtatcaaatt tgaaaaaaga gatatcttgg ttttacatgg
     4681 gaaaatatat gctattggat tgaaccttaa agagaaaata agaaaagaaa aattacttac
     4741 aaataaagag ttcgagataa taaatgccga taatatgtat atttccccag gatttataga
     4801 tgttcacacc cattgttata agagaaaatt acctacaggt atggattctg attcaattgg
     4861 aattgaaaaa ggttctacag ttatatttga tgcaggtaca gctggtcctt taaactacta
     4921 tgattttaaa aaaaagtata tggatgagtg taaaacagaa gtatattctt ttttaaatgt
     4981 tacaaatgag ggactaaata tattgaatga atcaacttct ttagatgtta taaattttga
     5041 caaggttgag tcagttgtag aaaaaaatat agaaaagata aagggtataa aagtaaaagc
     5101 gagtaaattt caatctaatg aaagtggtat agatataatt aagaaatcaa agcaagttgc
     5161 taaaaaatta aatttaccct tagtggtata tataggagag tatccatcat atatagatga
     5221 agtaatgaat attttgggaa aaggagatgt ggttacacca gtatatggaa atagtacaaa
     5281 tgggatcctt aaagaatctg gcacattaag aaaatcagta ataaatgcaa agaacagagg
     5341 agtattgttt gatgtatgtc atggattgaa ccaatttgat tttaaagttt ttaaaaaagc
     5401 aataaagcaa ggtttagagc cagacctaat aagtactgat atgcatataa aaaacatgaa
     5461 agacgtaaac tattctcttt taaatgttat aaataaaatt atggaacttg ggatatcctt
     5521 agaaaagtgt atagaaaaag taactgaata cccaacaaag attttcgatt tacatggaaa
     5581 taaaggcaga ataaagattg gtggagaagg tgattttaca atattcacaa tggaggagtg
     5641 taatgatatt ataaaagact acaataataa tgaacttgtt ttaaataaaa aattaagatt
     5701 gcaatatact gtaaaatcat ggatgaaaag cagtgaagtt tatagacatg gatacgaaaa
     5761 tactataata aactaaacaa tagtgataaa ataataaata tatgtttaaa ttggtatata
     5821 ttggataaat atctaaggaa acaacaatta aaaaataata attttagggg aggtatttta
     5881 gtatgaaagc atttgttgat aaggatgtat gtgtagcttg tggagcatgt ataggaacat
     5941 gtccagacgt ttttagtatg agcgatgaag ggcattcagt agcaattgaa ggagaaatac
     6001 cagcagactt acaagattta tgtaaagaag ccgaagaagg atgtccagtt tcagctataa
     6061 cagttaaata gagctgttta aatttatttt taaaataaat atataagatt taattgggta
     6121 cttaagctat agtattagaa atttctatta ctatagcttt ttaaaatata taaacttaaa
     6181 atataagata ttacttttta caaatatgga ggactgaaat atgaaaaaaa attatgaaat
     6241 agtattattc gatttagatg gaacacttac agaccctaaa gaaggaataa caaagtctat
     6301 tcagtattct ttaaatagct ttggcataaa agaagatttg gaaaacttag accaatttat
     6361 aggaccacca ttacatgata cttttaagga gtattataaa tttgaagata aaaaagctaa
     6421 agaagctgtt gaaaagtata gagaatattt tgctgataaa gggatatttg aaaataaaat
     6481 ctatgagaat ataaaagaga tattagaaat gctctataaa aatggtaaaa tattattagt
     6541 agcaacctct aagccaacag tttttgcaga aactatactt agatattttg atatagatag
     6601 atattttaaa tatatagcag gtagcaatct tgatggtaca agagtgaata aaaatgaagt
     6661 tatacaatat gtactagacc tatgtaatgt aaaagataaa gataaagtta ttatggttgg
     6721 agatagaaag tatgacataa ttggagccaa aaaaataggt atagattcta ttggagtgtt
     6781 gtatgggtat ggttcttttg aagaacttag tgaaagtgaa cctacttata ttgtcgaaaa
     6841 tgtagagtct ataaaagata ttttgttata aatatctttt atagacaaaa atattacaaa
     6901 atggtgtcgc atatttaaaa atatgatatt atagttaagg aagtcattta aaaaagagga
     6961 ggatgcactt aaaatgaaat ctattttaat tatgggtgga agcgacttca taggttctgc
     7021 acttgctaag cgcttgatta aatgtgggta tcaaattgat atacttacaa atggtaaaaa
     7081 ggaaatagat tataatggtt ttaaagaaca tctaatttgt gatagaaaag ttcgaaaaga
     7141 tatggaaaac attatcacag gaagaaaata tgattacata tatgatatga cagcatatac
     7201 aaaagaagat gtaagtaatc tgattgattt tataagtatg gataacttaa aaaagtatat
     7261 agtattgtca gcaggagcag tatataaaga tagtggaaga aatataaaag aagaaaatga
     7321 gaaaggtgaa aatgaaaact ggggaaaata tgggttaaat aaaaaagagg ctgaagattt
     7381 tattataaat agccctatac catatataat tattagacca acttatatat atggtgaaaa
     7441 taataattta tatagagagt attatttctt tgaaaaaata gaaaaaaatg aaaagatacc
     7501 agtaccaaaa ggaaaacaag ttagtaatca atttatatat attggggatt tagtaaaggt
     7561 attggaaagc ataatgaaaa atcctcatgt aagagaggcc tataatgtga caaatccaca
     7621 gttaatttct tgggatgacc ttatatatac atgtggagaa attattggca aagaacctat
     7681 aataaaatat gtagatatgg aaaaagtaga gtttagagaa agaacgtatt tcccatttag
     7741 aaatatagac tttaatcttg atataaataa gcttattgaa catggtcttt atataccaaa
     7801 tgtgttacta aaagaaggtc ttacagcaac atataaatgg ttttcagcaa ataagccaaa
     7861 aatgcatgac agaaaaatga acaaagttga gtctgtaatg caaatagttt aaaaaaataa
     7921 ataagtaggt agatattttg aactcaaaaa ttaagtattt tttaaaattt tttatcttaa
     7981 tatttatttt gatttttata tactcagttg cgatagagcc gagtctatta attgtgaaaa
     8041 aatataatat aagtgaaaat ggaacattga taaaaagtga tacaattgag agtaaatctg
     8101 aaaggataaa agtagttcaa atttcagata ctcaaatagg tagtttttat tcaactaaga
     8161 atctcaaaaa agtagctaat aagattaaca ctttgaatcc agatataata gtatttactg
     8221 gtgatttaat tgattactcc aataaaaatc caagtgtaga tgaaattaca actattttat
     8281 caagtatgaa tgctaggttg ggaaaatttt cagtatttgg caatcatgat tatatgtata
     8341 aacttcctag gtattatagg cagattatga aaaattccaa cttcaatctt ttagtaaatg
     8401 aaaataaaaa aataaaatta aaagatgata aatatattaa tatattaggt gttgatgaaa
     8461 tattaaatgg aaatcctaat ataaaatatc tggagagtca aatagataat aagaatttta
     8521 atttattgtt agcacatgaa ccagatttag tagatatgct tagtaaagat acaatgaatt
     8581 tagttctatc tgggcatagt catggtggtc aaataagatt acctattaag ggagcattag
     8641 ttactcctcc atatggaaga aaatatacta aagggtttta tgatataaat ggaaatcatt
     8701 tatatgtgag ttcgggtctt ggaagtacaa agttaccgtt tagatttttt aatataccag
     8761 agataataga atttaatatt ataatctaac ataaaatatt tagaaaatat aattttgctt
     8821 tttataatat gtaaatgtat atactgtaaa aatgggt
//
LOCUS       DAEMAE010000154         8760 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEMAE010000154 DAEMAE010000000
VERSION     DAEMAE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.156"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..455
                     /locus_tag="KPH93_002728"
     CDS             <1..455
                     /locus_tag="KPH93_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689529.1"
                     /translation="LKGVGDNSQMIKSLGINIGSIKILGLMISNGLIALSGSLMAQFL
                     GFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGML
                     STDLKLITAIVIIAFLATGNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            442..1197
                     /locus_tag="KPH93_002729"
     CDS             442..1197
                     /locus_tag="KPH93_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1689530.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1328..2350
                     /locus_tag="KPH93_002730"
     CDS             1328..2350
                     /locus_tag="KPH93_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1689531.1"
                     /translation="MINKKRLASLILAGALSVSMLTGCSQGGDSGNSKQESNSKSKEV
                     KKIGITQLVEHPALDATKAGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILMTAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNDNISY
                     VKTKQE"
     gene            complement(2514..3071)
                     /locus_tag="KPH93_002731"
     CDS             complement(2514..3071)
                     /locus_tag="KPH93_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG1689532.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REGILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(3077..4153)
                     /locus_tag="KPH93_002732"
     CDS             complement(3077..4153)
                     /locus_tag="KPH93_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG1689533.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEGLNEKLCFELINIVENPFTYTIYVRKTDLSNECIPIQIELEKSKMRFKK
                     GDSIYLDFPHEYLFCFRYNYNKKE"
     gene            complement(4154..4837)
                     /gene="modB"
                     /locus_tag="KPH93_002733"
     CDS             complement(4154..4837)
                     /gene="modB"
                     /locus_tag="KPH93_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG1689534.1"
                     /translation="MGTDWSPLWISLKTSILSTVITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVIIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4986..5798)
                     /gene="modA"
                     /locus_tag="KPH93_002734"
     CDS             complement(4986..5798)
                     /gene="modA"
                     /locus_tag="KPH93_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1689535.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTNKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLISDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDATKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5991..6920
                     /locus_tag="KPH93_002735"
     CDS             5991..6920
                     /locus_tag="KPH93_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689536.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTVKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNDRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(7093..7542)
                     /locus_tag="KPH93_002736"
     CDS             complement(7093..7542)
                     /locus_tag="KPH93_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689537.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGLLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFVLFIVECVYLVGRYILKVKINWSEIIENEKDNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKGRVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7749..8729)
                     /locus_tag="KPH93_002737"
     CDS             complement(7749..8729)
                     /locus_tag="KPH93_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1689538.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLAKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHTELNSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPYILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKELWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3038 a         1416 c         1073 g         3233 t
ORIGIN      
        1 ctttaaaagg tgttggtgat aactctcaaa tgattaaatc tctgggtata aatatcggtt
       61 ctataaaaat actaggactt atgatttcaa atggacttat agctttatct ggtagtctta
      121 tggctcagtt cttgggattc tcagatgtaa atatgggtat aggaacttta gttcttggaa
      181 ttgcctctat cataattggt attacactat ttaaaaaatt tacttttatc aaagatacta
      241 ctgcaataat tgttggctct tttatatacc aattcactat atactttgca atgagtttag
      301 ggatgttatc aactgactta aaattaataa ctgccatagt tataatagct ttcttagcta
      361 ctggtaactt aaatatttca ctaaaaaaaa acaaatgcaa agctagtacc aaaaataaat
      421 cagaaaaaag aggtgttgta gatgttacaa attaaaaatc tttcaaagag tttccctaat
      481 ccatacggtg aacctaacac tatatttgaa aatttatcaa tcgatataga agatggtgaa
      541 tttgtaagta tcataggtag taatggaact ggtaaatcta ctttattaaa cataatatct
      601 ggacttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
      661 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      721 tgtccttcta tgactgttag ggaaaacttg tctttagctc ttaataaagg aaaactatta
      781 aatttaagaa aatgtcttcg ttacaaaaga gattttttag aaaatttatt ggaaggtgta
      841 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      901 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      961 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
     1021 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcag
     1081 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
     1141 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
     1201 aatatatcta ttgtattaaa ctcaacttta aaataaagtt aaagctataa taattatggt
     1261 tactttgtaa taattcatat gaatatatga acatatataa taataaattt ttgaggggga
     1321 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtgt
     1381 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
     1441 ttctaaaagt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcact
     1501 agatgccact aaggcaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
     1561 aaatattgat atagacttcc aaaatgctca aaatgatatg cctactacac aaagtattgc
     1621 aagtaaattt gcatctgata agaaagattt aatatttgct atatctactc cgtcagctca
     1681 ggcagcattt aatgctacta aagatatacc tatacttatg actgctgtga gtgaccctgt
     1741 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1801 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1861 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1921 agaatatgca tctaaaaatg gctttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1981 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     2041 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     2101 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     2161 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggtgaatc
     2221 agtatctgat atgcctgtag ctacatcaga tgacacagat atcattgtta atgaagatat
     2281 attaaaagct ttaggtatgg aaaaaccttc taatgataat atttcttatg taaagactaa
     2341 acaagaatag aaagatattt tctctatacg attaatatgc ctgtttctat aaattaattt
     2401 atagaaacag gcatatattt ttattttaat gtattatata tttaaagatt atatgtaaat
     2461 actcttaatt tgtttttata aattttactt gtataaactt tatacaatca tatttattct
     2521 tctatcttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     2581 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2641 atgattactc ctccacctat ccatacatta tcacctattt ttacaggaga gccatactct
     2701 atacctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2761 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2821 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatgttt
     2881 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat accttctctt
     2941 ttttcatatt catcttctaa agtattatta aacaatctag ttaatttttt acaatactct
     3001 ctttctttta ctagcaattc atcatttgca tagtatcctt tcccggacaa cattttttct
     3061 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaaacaa aataaatatt
     3121 cgtgtggaaa atctaaatat atgctgtcac ccttcttaaa tctcatttta cttttttcca
     3181 attctatttg aataggtata cattcgttag ataaatcagt ttttctaaca tatatggtat
     3241 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaacctt
     3301 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     3361 ctatttctct gtttaatgtc aactcgcatc cccaatctgt agcataaata gtatttttat
     3421 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     3481 ttggagattc aaagagttct tttttaggtc tctttggaag gccttctccc ttattataca
     3541 ctataatatc atcacaaact cggtaggctt cttcaatatc atgagtaaca aatagtatat
     3601 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3661 ggtccagtgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3721 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctaccctcaa
     3781 acccatttaa tttaagtata tctaaatacc ttgctactat ttcctttttt tcactcttgc
     3841 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3901 agttttgaaa taaaaaacct acctttctat cttgtgttga taaatttatc ttcttttcag
     3961 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagacctg
     4021 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaaa cccagagtac
     4081 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcta
     4141 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     4201 aatttagtaa taaaatcatt gctatagata ttgctacaat aattataacc cacatcatag
     4261 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     4321 ttccaggtat atttccagct atcataagtg tagcaccaaa ttcgccaaga gctctggcaa
     4381 acgacagcac caatccacca attattccag gccatgctaa tggaactgct atcttaaaaa
     4441 atactttcca ttcattaaga cctaatgtcc tagcagctga caacatatta ttgtcaatct
     4501 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     4561 ttacagttgc tgtccaacta aagattaaag tcctatcaaa atttaataaa aactttccta
     4621 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4681 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4741 taatatatga cacaaatatt cctattacaa aagttattac tgttgacaaa atagatgtct
     4801 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4861 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4921 ttattttcaa ttttcattta tttatatata atttatactc aattatacat aatttattta
     4981 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     5041 aataaaaatt cttcaaattt cttagtagca tctacgtttt tactagcttt aattactgaa
     5101 actggatatg taataggtga atgagttttt tcgtctgttt tttcaactac ttttatctta
     5161 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     5221 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     5281 aaatttaaat tagttaacac ctcatctgca tatttaccag ctggaacact ttcagcttca
     5341 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     5401 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaatt
     5461 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     5521 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     5581 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5641 aaacttgcag cagccgatat atttaactct actgaatcac tacttttatt tgttttttct
     5701 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5761 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5821 ttgatacata ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5881 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5941 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     6001 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     6061 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     6121 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tgttaaagta aaggatacac
     6181 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatc tctggtcaag
     6241 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     6301 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatgac agagtttcta
     6361 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     6421 agctattgcc aggaatacca tgtgtactca taaatctagc ttatagaatg caaggtttct
     6481 atgttgctaa aggaaatcct aaagaaataa caacctggga ggatttaaca aaaccaagca
     6541 taactattgt aaatagagaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6601 gacttctaaa ctttaatggt aagcatataa aaggttatga taatgaagag ttatcacatc
     6661 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6721 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6781 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6841 ctcctgagtt taaagctgaa cttcaaggcc ttggcggata tgatttaact gatactggaa
     6901 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6961 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     7021 gtctttcatt atgtctttta ttttatcatc taatacaaaa tttaatataa ttaattgtat
     7081 aatcatatta tattaatata tatttacata atagtatttt tctttaatat agattaacat
     7141 atgatatgtt ttaaacatca gtacccttcc tttcttatat atatgtggca acacatatga
     7201 gtttaataaa ttatatacaa tattgctttc ttcttgtatt ttttcaacca acaagttatt
     7261 tataaaatta tctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat
     7321 atatcttccc actaggtata cacactctac aataaataat acaaatacaa tgcagtcagt
     7381 acctgttgtc ttatataaat tacccgatac taagggcttt gcgaagaaat tataaaaatt
     7441 caaagtatca cctgtataat caaaaagtat taatagtagt cctagaactc tcaatacttt
     7501 aatattcaac atgttcttat ctttattatt tttgtttttc atatatacta cctctctttt
     7561 ctttagattt tattgtaatc caatactaac tcttttattt gaggtataaa tgaatttaat
     7621 tggtagttat tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt
     7681 aaacataaaa atagcctatc tattttttaa tatataaata caaaaataaa taagctacta
     7741 atattcaact aacttttatc acttaaaaca ttactatcta taaatttaaa tattttattg
     7801 aagtatactg tactatcatg ttcaaatcca tcaacatgac caataccttc tataagccac
     7861 agctcttttt tatcatgttt aactaaatca tacattttct tgctattttg ataattacaa
     7921 acagtatctt ttgttccatg tataaacagt atgggtacat tagatttctc aactgactca
     7981 tatggtttta catcactgta ccaaaatcca gatttaaact tattatataa atctccacaa
     8041 atcactacat aattgattaa tatatacggt attcgttttt ctagtactcc cattctaatt
     8101 gcatctttca tttcagaata aggagaatcc agtatataaa aatcgacctt atcatctttt
     8161 gaatttagct ctgtatgcat agcagctgtt cctgccccca tggaaaatcc atgcactcct
     8221 aatcttcctt caggaaattt attttttaca aatttagcta gactatctaa atcatacctt
     8281 tcataaagac caaatgtgta attatctcca ccactattac cagtatttct ttgattataa
     8341 actaatacat tataaccttt atctaaatat ctatatgcta cctttaacat ttcatagtaa
     8401 ctacttccaa ttccatgaac aagtatcata gtatctcttg tctttatatt tgaagttata
     8461 aaaagacttt cgagcttgta attattagaa ctttgtatca ttaattcaga tgtcttgtag
     8521 ttttttaatt tctccagtgg tttatctttt ctctcagaaa atatttttac tatatcttcg
     8581 ttttttatta actgctctaa accaaccgaa ctattataca ctaatttgcc aaatatataa
     8641 gatacaatta ataggattaa tagaaacaca attgatataa ttataaattt atttcttaag
     8701 ctaatacaat ttttaaaact atttttcata tttatttacc atacaatttt cctatattat
//
LOCUS       DAEMAE010000155         8745 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEMAE010000155 DAEMAE010000000
VERSION     DAEMAE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.157"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..461
                     /locus_tag="KPH93_002738"
     CDS             <1..461
                     /locus_tag="KPH93_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689539.1"
                     /translation="YIYKLNINEDGIYNIILHWISEYDYNDIDTLTKINNLEIIFTVI
                     EKVVRGLFVEMLCKLQNICKINDSTKLHNKLQYIKDILYNGLKENIFLEKLNYKLLDR
                     RLTKKINLYMKKNAELYESRVETILLNIREYVDLDEFELFIIDKLIQKIK"
     gene            645..1320
                     /locus_tag="KPH93_002739"
                     /pseudo
     CDS             645..1320
                     /locus_tag="KPH93_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            1314..2709
                     /locus_tag="KPH93_002740"
                     /pseudo
     CDS             1314..2709
                     /locus_tag="KPH93_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            2970..3224
                     /locus_tag="KPH93_002741"
                     /pseudo
     CDS             2970..3224
                     /locus_tag="KPH93_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3234..3413
                     /locus_tag="KPH93_002742"
     CDS             3234..3413
                     /locus_tag="KPH93_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689540.1"
                     /translation="MIHVEISEKEFFRKIEEAFNKGEGNVEIDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            3425..3778
                     /locus_tag="KPH93_002743"
     CDS             3425..3778
                     /locus_tag="KPH93_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG1689541.1"
                     /translation="MRKYKAILLTLIISIVAIIGFIIYNNYFIKWNEVEKYIDKYMTY
                     QVVLDDDIERITKETYTKENYDGILYKSFYKNYPDYEYQYFYSGDYYALHVNKVMLQA
                     YHEEDGSKLQKIIKN"
     gene            3853..6408
                     /locus_tag="KPH93_002744"
     CDS             3853..6408
                     /locus_tag="KPH93_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1689542.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDGKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKATLVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDKLASKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLEDPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            6425..7087
                     /locus_tag="KPH93_002745"
     CDS             6425..7087
                     /locus_tag="KPH93_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689543.1"
                     /translation="MKLLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGDVFMDNLDLYTLSDDKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTDEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(7376..7486)
                     /locus_tag="KPH93_002746"
     CDS             complement(7376..7486)
                     /locus_tag="KPH93_002746"
                     /inference="COORDINATES: protein motif:HMM:NF025126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689544.1"
                     /translation="MKHSINFKRFLSRGNQNVLSEFILVVLAHNISKFHK"
     gene            complement(8099..8671)
                     /locus_tag="KPH93_002747"
     CDS             complement(8099..8671)
                     /locus_tag="KPH93_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG1689545.1"
                     /translation="MKILVKILINIICIITLIFSSLSAYIKLSEYRKADEVYTELREN
                     TSNNNSKHQELYDKNNDYRFWLKINNTNIDYPVVQGYNNDFYLTHNFYKNYLPSGNIF
                     MDYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEALFKENNLVETKYKVQTYTYKIF
                     SIYTADLIYRDYLSIHFNNDEFKDSLNYIT"
BASE COUNT         3639 a          871 c         1285 g         2950 t
ORIGIN      
        1 agtatattta taaattaaat ataaatgaag atggcatata taatataata ttacattgga
       61 taagtgaata tgattataat gatattgata cattaactaa aattaataac cttgaaataa
      121 tatttactgt tatagaaaag gtagtaagag gtttgtttgt tgaaatgcta tgcaaattac
      181 agaatatatg taagatcaat gacagtacaa aattacataa taaattacaa tatattaaag
      241 atatattata taatggactt aaagaaaata tatttttaga aaaactaaat tataaactac
      301 tagatagaag gttaacgaaa aaaattaatt tatatatgaa aaaaaatgct gaattatatg
      361 aaagtagagt ggaaactata ctactaaata ttagagaata tgtagattta gatgagtttg
      421 aattatttat aatagataag ctaatacaaa aaattaaata ataaatccaa tcgtaggtgt
      481 tatccctatg aatcgtatcc accattgaaa tccacgaata cctatgtcca attggatata
      541 ggtattttta tacattttta atttaaatat tattgataaa tacatttaaa aagtgttaac
      601 ctaaagtgaa gaatctaaat aaaaatacaa tattaggaga ggttatcatt acaatagcag
      661 cttgtgatga tgaaaaagaa atacaaatcg taataaaaaa tcaagttgat aatatattaa
      721 aaaataaaaa caaagaatat cgaattaaaa aaatatagct caaaagaaga accattacaa
      781 agttaattga gaggatacaa tactcttaga tataaaaatg gcagaactaa atagtataga
      841 tatcgcaaga aacataagaa aagtagatga taatatttat aacatctcta ataaattatg
      901 tacaggaaag atatgaagta agagcttatt gatacttatt aaagccaata aattttcaag
      961 aactagaaaa acacatatta acatgtatca aaaaaattga aaaaaataaa aataagtcta
     1021 acacatacaa aatctactct agcaaaaaaa tatatagaag ttcaaaaaaa gaacatgcta
     1081 agacatacaa aaaataaaac ttttgatgct atatgtatcc tagaaaaaat agaaaaagat
     1141 ttaaatatag aggaacttgt aagatgtcat aaaagttata taattaactt aaactatgta
     1201 gaaaatataa agtcaaatac tgcaatatta gacagtcaag aagaagtacc tattagcaga
     1261 tatagatata aagatataaa agagagattt ctaaaattta ttggtgatag aatatgctaa
     1321 accatataaa taaaatatta ttaatattat cattgtttac accaataatg atactaaaaa
     1381 gtatattaac ttacgataaa gatacagata agactaagaa aatatctttt tttactgtat
     1441 tattagctta tcttataact aaaatcatta tattatttaa cgttgataag acccatgatt
     1501 ataatatgaa gaaaaatcta tatgatataa tctattattt gatatttatt atttgggaac
     1561 ttactataat tatttttata agataggaat tgttaaatat atagtttcat ttttcttatt
     1621 taacgtatat caccctttaa taaaagatgt tatatctata aatttatttt ttgtagttac
     1681 aagtaatgta atatcatcag aggaagctct attagtgtct agttatgata atgctaaagt
     1741 agatatatac tattatctta taataatagt tatatatagt agcttgtgct atatttaaat
     1801 ttattaataa aagattggga ttagataaag ataataaaat aaactatata tacttatctg
     1861 ttgctttaat aacaaattta tttactatat taataattta tataaataga aaaatgtatc
     1921 gattttgtgg gtattaggaa atatatatag ttgggtatat tgtaatacct aggttaatac
     1981 tagctagtag cttagtaatt attatagtgt ttataaaaat aattaaggaa aataagttaa
     2041 aatcgcaaaa tgaagtaata aagaataagc tagatatgca gtattctcat tatctaagta
     2101 ttcaagaatc acacactaaa gttaagaagc tttatcatga tataaataat catatatatt
     2161 gtatagaaaa tctcaaatat aaaaatagga aaattgatgg gaatgtaaat attttaaaag
     2221 atgaaattaa ggaatttaga tcaatttaca atacaggtaa tgttatattg gatataataa
     2281 taaatgaaaa aagtgatgta tgtgaaaaaa gaaaaattaa attcatttgt gatatcaatt
     2341 tttccaaagt gaattttata aaacaaatag atgtatctag tatatttgca aatacttaga
     2401 taatgcaata gaagcttgtg ataagataca tgataaaagt attgataaat atatacgaat
     2461 aaaaggaact atctcaaagt cctatttcgt aattaaatgt gagaatagta aaataaattc
     2521 aattagaaag aataaaagtt tattattaac agataaaaag ataaacttgt acatggaata
     2581 aggcttcaaa gtatcaaatc ttctataaaa aaatatagag gagatttatt atttgaagat
     2641 aaaaaagaca aatttatatt gaatatttat acaccattaa atcaaaatat tgatagttgg
     2701 gtcgattaac ctaccagttg gggaacatat attgatacta aacatatagg gatgttatct
     2761 tttaatagaa aaagatagcg tctttttttt atgagaaata acgagttttc aatatataag
     2821 gaggattata ttaagataaa aaaattataa tagctatgat tatagctata agcttaacgc
     2881 taggtttaat aacaggatgc acaaataagg tttcagatac aaatgaaaaa atatatacca
     2941 ttgaacagat ttctagtatc aaggaagact tgtcaaagaa aatatcagaa tcaatagaaa
     3001 atagtggtct tagagtagtt gaaaataaat cagatggaag ttttgttttg tctttgggaa
     3061 acttggagta tacaaaagaa caacctagcc aagttttaca ttattcaatg actaaggaca
     3121 agaagggtac gaaggaaata ttatcaattc aatgtgttaa agatttttct tataatgaaa
     3181 agctgtcaga agatgataaa ttcataatgg ccatttataa catatataga tcgttgattc
     3241 atgtagaaat aagtgagaaa gaatttttta gaaaaattga agaagcattt aataaaggtg
     3301 aaggaaatgt agagattgat aatttgggag atataaaaat tcaagttaag aaatttgatg
     3361 ataaaactaa gtctttagaa ttaaaactta ttaaagaatt taaattgaag taggaagatt
     3421 cactatgaga aaatacaaag ctattttatt aactttaatt ataagcattg tagccataat
     3481 aggatttatt atatacaaca actattttat aaaatggaat gaagttgaaa aatatataga
     3541 taaatatatg acatatcaag tagttttaga tgatgatata gagcgtatta caaaagaaac
     3601 gtatacaaaa gagaattatg atgggatact ttataaatca ttttacaaaa attatccaga
     3661 ttatgaatac caatattttt atagtggtga ttattatgca ctacatgtaa ataaggtaat
     3721 gctacaagct tatcatgagg aagatggtag taagcttcaa aaaataatta agaattaaaa
     3781 gatttaaaat atccaccaat ctactcaata gatgagttgg gtaaataatt tatagtaaaa
     3841 taggaggatt atatggggat ttacttaaaa atgtcactag catatttaaa gaaaaataaa
     3901 cttagaacgc tacttttaat aataggagtt atgttaggtg ttatgttgat atttggacct
     3961 agtgttataa aagattcaca aaatgaaaac aatgtagtag caatacatga actttacagt
     4021 ggataccatg tagagtttag tgatttgaca cttgatgatg ctaaaaagtt agaaactgat
     4081 gggaaagtat caaagggtaa ttcggtacaa aatctagggc gtatttcaga taaaaaagga
     4141 aattcattta tattaacttc atcaaacgaa gagtatctaa aaagtagata caacaaaata
     4201 atagaaggta gactacctaa aaacaacaat gaaatagtat tagagaaaaa agcactagaa
     4261 gcaatgaatt taagtgaagg agtaggatct acattaaatt taacaataaa aaagaactat
     4321 aaagatacta acggaagtaa tcaattattt tctataaata aagaatttaa aatagtagga
     4381 gttatggaaa aaccaaaagg atactatgat gtggtttggc aatacgaagc agtaacttat
     4441 ggcaatgatg aaacaaacaa cattatacct catgatgcga ttagttataa tagtatatta
     4501 agctttaaaa gtggatggag aaatattgaa ggtgaatgtg attatcttat gaaaaaatat
     4561 aagttaggac aaaaaagttt tctaccaaat gtacctcttg taagggaact tcgttatgta
     4621 gaagatgata agaatgatcc gaatgttttt aaaagaaatg ttttaataac tacagtatct
     4681 ataattttca tatttaatat tttcaatatt acactaaatc agtcagtaaa agaaatggga
     4741 ctgcttagac tgatgggcgc aaagaaaaga aacgtaaggt gtatgataat ctatcaagca
     4801 ttaattataa caataatagg tatactactt gggttagtag ctggggttac atattcatat
     4861 ataggaatta gtaattataa cgaagtttta tacagtgaag ctggtcttaa ccctaaatta
     4921 tatataaata atgaaatagt tataaaagct acattggttg gaataatttc tgtttttata
     4981 tcttgtatta taccaacttt taaaatcggc aagatatcac ctattgaatc aataataaat
     5041 acagacaaaa ttaaaaatca cagaagtaga cttggaatag acaaactagc aagcaaaata
     5101 tttggatttt atggatttat gggacttaaa aatatcggaa gaaataaggt tagagctgct
     5161 atatctatgt tgtctattgc tttaggagga tatatattca ttactacatt ctcaagtatg
     5221 caagaagagg tcaacagtaa aattgaagat atgcaaaata gatataatat aacaatgcaa
     5281 tttggagtaa atgcagaccc gcaaatatca aaatacacag atagtgatgt agagaatata
     5341 gaaaaaatta agggtgttaa aagtatacat aaaattcaaa taacaaatgg aatgtttact
     5401 tttaataata aggatataaa caaagaattt acaacctata atggagtaga aaaaaaagag
     5461 ataatggaag ataaattagt tttaaagctt tatgatgatg attatataga tgaaaaatta
     5521 aatgactttg ttgaagaagg tagcttagat gatattaaaa aggacactga tggatatcca
     5581 aatgtagcag tatataatta cttttatgat atagttgatg atcatactat aaagcctata
     5641 ttcaaagaat taaatatagg agacataata actgtaaaaa ttcctgttga agaaaataat
     5701 acgattgttt ataaagagat gaaggtaaga gtggctgtaa ttttaaagga tgaatggatt
     5761 gctatgggag atggcgattt tatgccagat ctagaaatta taacaagtaa tattcactct
     5821 aaaaacataa caggagaaca aaaatacact caattaggca taaatttaga agatccatat
     5881 gatgagattg ttaataatga gatacagaaa atttcaagaa gtataccagg gtctctattt
     5941 aatagtagat taagttatca tgaaatgtca gaaagtatat ataaaagtta cattaaatca
     6001 caaatagcaa taattgcact agttttaata atagcatcaa ttaatatttt ctgtactata
     6061 agaacaaatc tactaattag aaaaaaagaa atttcaacgc ttagggcatt aggtgttagt
     6121 cttaaaaata tgaaaaaaat gcttatatat gaagcattag catatgcagt ttttagcttt
     6181 ataatagcta tgattccatc atttataaat ctagttaaat ttacaaattg gaataatgat
     6241 gcgtatataa actatggaat agagaatttt atgtctttta cattcccaat taaggaatct
     6301 ttactattct ttgctatatc tgtatttgta tgtttaatag cagtagttgt aagtaataga
     6361 gattttgaaa gtatgaatat tattgaaggt ataaaagaga atgaataatt tgggaggtaa
     6421 atgtatgaag ctgttaaaag ttatagatat taaaaaaata tatggaaaag gagaaaatga
     6481 aataaaggct gttgatggaa ttactttaga gatagaaccc cataaattta gtgcaataat
     6541 tggacaaagt ggttctggaa aaagcacatt acttcactgt atggcagggc ttgataaacc
     6601 aactttaggt gatgtattta tggataattt agatttatat actttaagtg atgacaagct
     6661 atctaatctt agaagtaaag aatttgggtt tatattccaa aactataatc taatacctgt
     6721 tatcaatgtt tatgataata tagttcttcc tattttaatt gctaaaggaa agatagataa
     6781 taattatatt aatgatttaa taactaagtt aggattagaa agtcagatta ataaatttcc
     6841 taatgaatta tcaggtggtc aacaacaaag agttgcaata gcaagatcac ttgccaataa
     6901 gccatcaata atttttgcag atgaacctac tggaaattta gatagtaaaa caactgatga
     6961 ggtgatgaat ttactaaagt attgtgtaaa tgaatacaag cagacgttgg ttatgataac
     7021 tcataatgat gaaatagcta agatggctga ttgtataata actattagtg atggtaaagt
     7081 catataaaat gcattgtata aaggtttaat cgtgggtatt attcttgtga ttcttcccca
     7141 ccatttaaat ccacgaaaaa aggactgtca aatgagtaat tttaaattgc tgtgcgacag
     7201 ttcttttttt acttgttttt cattaattta tatttatgca aaatttgagc ttaaaacggt
     7261 cataagaaat aaaggtataa aaataccatt ttaaaataga ataatttaca aattgtaaga
     7321 ttaaaaaata tttttgagag gatgaatata catactacat ttattaatca ttatcttatt
     7381 tgtgaaactt agaaatatta tgggctaaca ctacaagtat aaactctgaa agaacatttt
     7441 gattgcctct gcttagaaat cttttaaagt taatactatg ttttatttgg gcgaaagctc
     7501 cttatgcctg aatacttcta ttcattctaa gttcacatac ttgtgtacca gtgattcttt
     7561 ctaaattatc ttaatgccaa aatcttgttc gcatttcaaa ataaagtcag gtagctactt
     7621 taacatttta ggtatatttc ttcaatagtt tgtctccaaa caaaggtaca tctattagca
     7681 acagatttaa ttttacttat atcaataaat aatatatcct ttgaaacttc attaatgtca
     7741 ccaagataat ttataagtta agctattgta tactatcttc accaactaca tcctcaatag
     7801 ttttatactt tcttacttca gcttggtaat ctaaatctgt aataattatt tctctttgat
     7861 tttcatctct ttgcctacaa aagtttaata tgaatattat tccataatta tcaattttaa
     7921 gttcaaaaaa gattgcaggt aagtctgtat ttacacaaac ctacttatca gtctcttttt
     7981 ttgaaattaa tttagcatga actatagtac gagcatctct aaattcatag ttataagtat
     8041 acaaagttat aattttatct gaaatatcta cctttatatc agatttgtgt actgatcttt
     8101 aggttatata attcaatgaa tctttaaact catcattatt aaagtgtata cttaaataat
     8161 ctctgtaaat taagtcagca gtatatattg aaaaaatttt ataagtataa gtttgtactt
     8221 tatattttgt ttctactaag ttattttctt tgaaaagtgc ttcatcagta tagttttcta
     8281 gttgaccaaa catagtttta tttttcatat aatgaccata gactattaaa ctcttatcat
     8341 tttcaaagtt atttctataa tccatgaaaa tattacctga aggaagataa tttttataaa
     8401 aattatgagt taggtagaaa tcattgttat atccttgtac tacaggatag tctatatttg
     8461 tgttgtttat tttaagccag aatctataat cattattttt atcataaagt tcttgatgtt
     8521 tactattgtt attgctagtg ttttctctga gttcagtata tacttcatca gcttttctat
     8581 attcagataa ttttatgtaa gccgaaaggc tagagaaaat tagtgtaata atacaaataa
     8641 tgtttattaa tatttttact agtattttca attaatcacc ccctgtattg ttatataaaa
     8701 gattaaataa attttatata gttaaaattt ttattatagt tattt
//
LOCUS       DAEMAE010000156         8726 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEMAE010000156 DAEMAE010000000
VERSION     DAEMAE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.158"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..795)
                     /locus_tag="KPH93_002748"
     CDS             complement(64..795)
                     /locus_tag="KPH93_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1689546.1"
                     /translation="MKFSHKLNNLSHAEKHIFYYIDSSIHTVKDLSLTALADINNVST
                     TTIIRMCSKIGLSGYSELKHILKDLDKESTSISSGYHVSNIIDNLNQNLSNLNLTNIN
                     KLAKNIKSANRIIIVAVGLSKPIGEYFSKLLMQANKNSFYVYESHMIDLLDQTTEKQD
                     LVIFVSNSGETRTLITACEKFRYKNLNTAAIINSPNSTLSMLVDIPINTCSQKLNISG
                     YDTTPRSTILVIIDILFETYLNIIE"
     gene            948..2918
                     /locus_tag="KPH93_002749"
     CDS             948..2918
                     /locus_tag="KPH93_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1689547.1"
                     /translation="MNLAKMTNKNLIVLDVSVATKEEAIRLLIDKLYKENIISSKEKF
                     FDTVMEREKHSPTGLERGLAIPHGKCDSVNRSAFAVARLNNKINDWESIDESNEVDLV
                     FLLAIPKSEKETTHLKLLSELSVALMDENFYNDLSISKNADEFLKNLNRSDKEEKDTN
                     KYDKTVLVVTACAAGIAHTYMSAEALEKVGKEMGIKVISEKQGASGIEDRHTKEQIQK
                     ADGVIFACDVAVKNIERYQGKSFVKVRVAEPLKHSKNLLNKVLQNPDGKVEVLSNEEA
                     FTSTYELKQGTLKEMMEAVMTGISYMIPVIVSAGLMMGIAKLTAVAIGQIDNMGTFIE
                     SGNTFYELLGYLDMFGSMIFKFIYPIFSAYVAYSIADRPALVPGFVGGAFAGGLHFTF
                     WGVEGGVPSGFIGALILGLVAGYVTRFLNENIKLNKNLQAMKPMFLLPGITMLVIFLL
                     NFYAVDPAFGRVNAFIANIIESFGTASTIALTVVIAACTAFDLGGPVNKAAGAIAIGL
                     AADGVFPLTARVLSIVIPPIGLGLATVLDKIIVKRRVFDDNLRVVGNSSIILGLIAVS
                     EGAIPFMLKNPLITIPINILGAIIGSITAVMLGAIQWNPLPAIWGWPLVENLWAYLVG
                     LIVGTLFIALANIFIRFNIIKKQKKNDMNKTA"
     gene            3024..5702
                     /locus_tag="KPH93_002750"
     CDS             3024..5702
                     /locus_tag="KPH93_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG1689548.1"
                     /translation="MEEDMNNRKKVYVVTHSHWDREWYFTIEDSNILLSENMPYLMDV
                     LEKDMDYTSYTFDAQLSVVEELVKLYPEEKERLKKLVSDKRIFVGPWYTQTDSILVNK
                     ESIIRNLLYGTRLGNKYGHSMKVGYLPDIFGQNAYLPSIFNGFDIEYSILQRGIYIDD
                     LKGNLNFKWVSPDNESVRANNIFLGYGPGKFLSSDNQYIQDKLLPMLEKLESLNGDCD
                     NLLLPCGGDQVLVRKQFPQTVKELNEKQDKYEFVLSDYETFMKDTWINDFDNKIHGEL
                     IACEKSRIHNTIKSQRYDIKYLNTLVENKILYVLEPLSVIGKFSGISYKSRWLDIMWK
                     LIFDAHAHDSIGGCNSDETNRDIVTRLKKVNAMCDDIINIVKKQITMAISKYLNRDNI
                     LVVFNTKNKCMNEIIETVVFTKEKDFTINTIDKEIVEFTINNQEVISGGKQILVTAEG
                     EKEVELPGYYRTEIRLANVNVKPMGFTTLVVNQNSKEVADILVASNDRSIENDLYKLV
                     FNNNNLLLINKYTNKKIDNIVYFENCGDYGDSYDFSPLDNDKDILASKAELLEVSKSN
                     LVQTMKLKHSIELPFDIDERKLNKKSTILEIITRIEIRKGENFIRVSHDIENTVKDHR
                     VRVVYNTNIKSKVSLSDQGFSAITRQNCNRYLDNWKERKFAEKPVCIYGLENFVALRE
                     NSNTFALVTKGIKEYEILEDSKIALTLYRSVGLLGRDNLAWRPGRASGINNKVMYTKD
                     AQLFNKKLKFEYAIYYDKSDEDVATLFDVTDKFINKYTTYQNQDLNLFEERLERFEIP
                     KYHTINLSSYSLFNIDNDDVFMSVCKESYEEDGVIVRLFNPTNEAKEVSINSEYIKDI
                     KITNLYERELDTLDSNVQIKAKGYITLKLLGGGYNE"
     gene            5695..7389
                     /locus_tag="KPH93_002751"
     CDS             5695..7389
                     /locus_tag="KPH93_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBG1689549.1"
                     /translation="MNKTILLQDIEQRLKNIYKEKYKPKFFEMMKSTIEKFEDKDFDK
                     VDAISEKNVYLITYGDSIYEEGIPTIHTLKKFLNNKVGYTITDVHILPMFEYTSDDGF
                     SVVDYMKIDKNLGNWESIKNLSKDYRLMYDFVANHISKSSIWFQGYLRGEEKYQNYFI
                     PEDENFDYSNVIRPRTSPIIHKYQGKNGVKTAWTTFSEDQIDINVKHFPVLVETTEVL
                     LNYALNGASSIRLDAIGFLWKESGTTCIHLPQTHMIIQLWRVMLDYFKKDTQIISETN
                     VPHLENISYFGDTTNEAHMVYQFTLPPLTLHTFTTHDSTKLTKWASTIDKVSDNATFF
                     NFLSSHDGIGMRPTEGILTEEENKRLVDKTIINGGRVSYKDNPDGTKSVYELNINYLD
                     ALLNKDEDIDEDIQIQKILSAHALLLSFIGVPAIYYHSLLGSKNYYEGVEKSGINRRI
                     NREKLEYNRICYELEHDSRRNKIFTQLKNMIDIRKGESAFSPYANQNVLDFGREIFAL
                     ERENKETNEKVVFITNVTPNEKIIDCGFNGINILENTNIEGKLTLGAYKFAWIKIK"
     gene            7646..8356
                     /locus_tag="KPH93_002752"
     CDS             7646..8356
                     /locus_tag="KPH93_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689550.1"
                     /translation="MINIGICDDELHYRLKIKDILKKVLSSYTMDYNIHEFSSGKELL
                     CNYPKNLDILIIDIQMEILNGIDTSRKIREFDENLEIIFMTSFPEFMQEGYEVKAYRY
                     ILKPINEKKITKNILPCIDEIMKKRNNYLTIKVKNYVDRIKIDSIIYIETDRPNIVIY
                     THDNTYTTKMSISKIEKILSEFGFFRCHNSYIINLKLVQSMNGNSVVINGKSIPVSKY
                     RVKGLKLAITNILGDIIC"
     gene            8350..>8726
                     /locus_tag="KPH93_002753"
     CDS             8350..>8726
                     /locus_tag="KPH93_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1689551.1"
                     /translation="MLETLNINLWDIINIITGCIDWIIFFLVMSILGKKNISFRKYTI
                     YMTLVLVSMSLVNFMSIFPNFKIIICIVIGSLFYIASYKESIYKCILITLLFWLGLMM
                     GEGLAIGIVVVINQLNNIDIILNG"
BASE COUNT         3434 a          919 c         1536 g         2837 t
ORIGIN      
        1 ttaatatgtt ttatcctagt ggttttaaag aagacatttt tacttataac tatatctttt
       61 ttattattca ataatattta gataagtttc aaataatata tctataataa ctagtatagt
      121 agaccttgga gtagtatcgt atccagaaat attaagcttt tgagaacatg tatttatagg
      181 tatatctact agcatagata gtgtagagtt agggctattt attatagctg ctgtgtttaa
      241 gtttttgtat ctaaattttt cgcatgctgt aattagtgtg cgtgtttctc cactattaga
      301 tacaaatatt actaagtctt gtttttctgt tgtttggtcc aacaagtcaa tcatatgaga
      361 ctcatataca taaaaactat ttttattagc ttgcattagc agttttgaga agtattctcc
      421 aattggcttt gagagcccta ctgcaactat tattattcta ttggccgatt taatattttt
      481 agctagttta tttatatttg ttaaattaag atttgataga ttttgattta agttatctat
      541 tatattacta acatgataac cactagatat agatgtagat tccttatcta aatctttaag
      601 tatatgcttt aattcagagt aacctgataa tcctatctta gaacacattc ttattattgt
      661 ggtagtgctt acattattta tatcagctaa agctgtaagt gataaatcct ttactgtatg
      721 tatgctcgaa tctatataat aaaatatatg tttctcagca tgactaagat tattcaattt
      781 atgtgaaaat ttcattatca aactactaat attaatcacc acctttgtat atagtaaaaa
      841 agtacaaatg tactttttat gtataagaaa atgtttttca tctaatatat aataatatta
      901 taagataaat aagcatttaa gtaaatgtat taataggagg gtgagatatg aatttagcta
      961 aaatgaccaa caaaaactta atagtgttag atgtaagtgt agctacaaag gaagaagcta
     1021 taaggttatt gatagacaag ctttataaag aaaatattat tagttcaaaa gaaaaattct
     1081 ttgatactgt aatggaaaga gaaaagcatt ctccaactgg actagaaaga gggttagcta
     1141 taccacatgg taaatgtgac tctgtaaata ggtcagcttt tgctgtagct agattaaaca
     1201 ataaaataaa tgattgggaa agtatagatg aaagtaatga agtggattta gtatttttat
     1261 tagctatacc taagtctgaa aaagagacta ctcatttgaa gttattatct gaattatctg
     1321 tagctttaat ggatgaaaat ttctataatg atttaagtat ttctaaaaat gctgatgagt
     1381 ttttgaagaa tttaaatcgt agtgataagg aagaaaaaga tacaaataaa tatgataaaa
     1441 ctgtattggt agtaactgct tgtgcagcag ggatagctca tacatatatg tctgctgaag
     1501 ctttagaaaa agtaggtaaa gaaatgggga taaaagtaat atcagaaaag caaggtgcta
     1561 gtggtataga agataggcat acaaaagagc aaatacaaaa agcagatggg gtaatatttg
     1621 cttgtgatgt tgctgttaaa aatatagaaa gatatcaagg aaagtcattt gttaaggtta
     1681 gagtagcaga gcctttaaaa cattctaaaa atttacttaa taaagtactt caaaatccag
     1741 atggaaaagt tgaagtttta agcaatgaag aagcttttac atctacatat gagttaaaac
     1801 agggtacatt aaaagaaatg atggaagctg taatgacagg tatatcatac atgattcctg
     1861 taatagtttc agcaggtctt atgatgggga tagctaagct tactgctgtg gctatcggtc
     1921 aaattgacaa tatgggaact tttatagaaa gtggaaatac attttatgaa ttactaggat
     1981 atttagatat gtttggaagt atgatattta agtttatata cccaatattt tcagcttatg
     2041 tagcatattc tattgctgat agaccagctt tagtaccagg atttgtaggt ggtgcatttg
     2101 cgggaggact tcattttact ttctggggtg tagaaggtgg agtaccatca ggatttatag
     2161 gagctttaat attaggttta gttgcaggtt atgttactcg atttttaaat gaaaacataa
     2221 agttaaacaa gaatttacaa gctatgaagc caatgttcct attaccaggt ataacaatgt
     2281 tagtaatatt cttattaaac ttctatgctg tagaccctgc atttggtaga gtaaatgcat
     2341 ttatagcaaa tataatagaa tcttttggca ctgctagtac aatagcatta actgtagtta
     2401 tagcagcttg tactgcattt gacttaggtg gaccagtaaa caaggcagca ggtgctatag
     2461 ctataggtct tgcagctgat ggtgtatttc cattaacagc tagagtatta tctatagtaa
     2521 taccaccaat aggattaggt ttagctactg ttttagataa gattatagtt aagaggagag
     2581 tatttgatga taatttacga gtggtaggaa actcttctat aatacttgga ctaatagctg
     2641 tgagtgaggg ggcgatacct ttcatgctaa aaaatcctct aataacaata cctataaata
     2701 tattaggtgc aataataggc tctataacag cagtaatgtt aggtgctatt caatggaatc
     2761 cattgccagc tatatgggga tggcctttag tagaaaattt atgggcatac ctagtaggtt
     2821 taatagtagg tacattattt atagcattag ctaatatatt tataagattc aatattataa
     2881 aaaaacaaaa gaaaaatgat atgaataaaa cagcataata ataattggga attattcaga
     2941 taaatttaac tagaataatt cccatttgta caagtggata tttaataaaa ttatttagaa
     3001 ataattaaaa aagatgattt tctatggagg aagatatgaa taataggaaa aaagtatatg
     3061 tagtaactca ctctcattgg gatagagaat ggtactttac tatagaagat tcaaatatac
     3121 tattaagtga aaatatgcct tatttaatgg atgtacttga aaaggatatg gattacactt
     3181 cttacacttt tgatgcgcag ttatctgttg ttgaagaatt agtaaagcta tatcctgaag
     3241 aaaaagaaag gcttaagaag ttagtaagtg acaaaagaat atttgtaggt ccttggtaca
     3301 ctcaaacaga ttctatactt gtaaacaagg aatctataat aagaaatctt ttgtatggta
     3361 caagacttgg aaacaagtat ggacatagta tgaaggtagg atatcttcct gatatatttg
     3421 gacaaaatgc atatttacca tctatattca atggttttga tatagaatac agtatattac
     3481 aaaggggaat atacatagat gatttaaagg gaaatttaaa ctttaagtgg gtttctcctg
     3541 ataatgaaag tgttagagct aacaatatat tcttaggata tggaccaggt aagtttttat
     3601 catctgataa tcagtatata caagataagt tactacctat gcttgaaaaa ttagaaagtt
     3661 taaatggaga ttgtgacaat ttacttttac catgtggtgg agatcaggtt ttagtaagaa
     3721 agcaattccc acaaactgta aaagaattaa atgaaaagca agataagtat gagtttgtac
     3781 tatcagatta cgaaactttt atgaaggata cttggattaa cgattttgat aataagatac
     3841 atggagagct aatagcatgt gaaaagtcta gaatacacaa tactataaag tctcaaaggt
     3901 atgatataaa atacttaaac actttagtag aaaataagat tctttatgta ttagaaccat
     3961 tatctgtaat aggtaaattt tctggaataa gttataagtc tagatggtta gatattatgt
     4021 ggaagttaat atttgatgca catgcacatg atagtatagg tggatgcaac tcggatgaga
     4081 caaatagaga catagttaca agacttaaaa aggttaatgc aatgtgtgat gatattataa
     4141 atattgttaa gaaacaaata acaatggcta taagtaaata tttaaataga gacaatatat
     4201 tagttgtatt taacactaaa aataaatgta tgaatgaaat aatagagact gtagtattta
     4261 ctaaggaaaa agactttaca ataaatacta tagataaaga aattgtagaa tttactataa
     4321 ataaccaaga agttataagt ggtggtaagc agatattagt aactgctgaa ggtgagaaag
     4381 aagtagaatt acctggatac tatagaactg aaatcagatt ggctaatgta aatgttaaac
     4441 ccatgggatt tactacatta gtagttaatc aaaatagcaa agaagtagct gatatattag
     4501 ttgcatctaa tgataggagt atagaaaatg atttatataa attagtattc aataacaata
     4561 acttactatt aattaataag tatactaaca aaaaaataga taatatagtt tattttgaaa
     4621 attgtggtga ctatggagat tcatatgatt tctctccatt agataatgat aaagatatac
     4681 tagcaagtaa ggcagagtta ttagaagtat caaagagcaa tttagtacaa actatgaagt
     4741 taaagcacag tatagaattg ccttttgata tagatgaaag aaaattaaat aaaaagtcaa
     4801 ctatattaga aataataact aggatagaaa taagaaaggg tgaaaacttt ataagagttt
     4861 cacatgatat agagaatact gtaaaagacc atagagtaag agttgtatac aatacaaata
     4921 taaaatcaaa agtgtcatta agtgaccaag gattctctgc cataacaagg caaaactgca
     4981 ataggtactt ggataattgg aaagaaagaa agtttgctga aaagccagta tgtatatatg
     5041 gcttagagaa ctttgtagct ttaagagaga atagcaatac ttttgcatta gtaacaaaag
     5101 ggataaaaga atatgaaata ttagaggaca gcaaaatagc acttacttta tatagaagtg
     5161 ttggtttatt gggaagagat aacttagctt ggagaccagg tagagcttct ggtataaaca
     5221 acaaggtaat gtatactaaa gatgcacaac tttttaacaa aaaactaaag tttgagtatg
     5281 ctatatacta tgataagtca gatgaagatg tagcaacatt atttgatgtt actgataaat
     5341 ttataaataa gtatactact taccaaaatc aagatttgaa tttatttgaa gaaaggttag
     5401 aaagatttga aatacctaaa tatcatacta taaacttatc aagctatagt ttatttaata
     5461 tagataatga tgatgtattt atgagtgtgt gtaaagaatc ctatgaagaa gatggagtaa
     5521 tagtaagatt gtttaatcca acaaatgaag ctaaagaagt aagcataaat agcgaatata
     5581 taaaggatat aaagataacc aatttatatg agagggagtt ggatactttg gattctaatg
     5641 tacaaataaa agctaaagga tatataactc ttaagttatt agggggagga tataatgaat
     5701 aagacaatac tgttacaaga tatagaacaa agacttaaaa atatatataa agaaaagtac
     5761 aagccaaagt tctttgaaat gatgaaatct actattgaga agtttgaaga taaagatttt
     5821 gacaaggtag atgctatatc tgaaaagaat gtatatttaa taacttatgg agatagtata
     5881 tatgaagagg ggatacctac tatacatact ttgaaaaaat tcttaaacaa taaagtagga
     5941 tatactataa ctgatgttca tatacttcct atgtttgaat acacttcaga tgatggattt
     6001 tctgttgttg attatatgaa aatagataaa aatcttggaa actgggagag tataaaaaat
     6061 ttgtctaagg attacagatt aatgtatgat tttgttgcaa atcatatatc gaagagcagt
     6121 atatggtttc aaggatactt aagaggagaa gagaagtatc aaaattattt tataccagaa
     6181 gatgaaaact ttgattactc aaatgtaata agacctagaa cttctccaat aatacataag
     6241 taccaaggaa aaaacggtgt aaagactgct tggactacat tcagtgaaga ccaaatagat
     6301 ataaatgtta aacatttccc agtacttgta gaaactactg aagttttgct taattatgca
     6361 ttaaatggtg caagttctat aaggcttgat gctataggat tcttatggaa ggagtctggt
     6421 actacttgca tacatttacc acaaactcac atgataatac aactatggag agtaatgctt
     6481 gattacttta agaaagatac tcaaataata tctgagacta acgtgcctca tctagaaaat
     6541 ataagctatt ttggtgatac tacaaatgaa gcacatatgg tatatcaatt tacattaccg
     6601 ccattaacgc ttcatacatt cactacacat gattctacca agcttacaaa gtgggcttcg
     6661 actatagata aggtatctga taatgctacg ttctttaact tcttatcaag tcatgatgga
     6721 ataggaatga gacctacaga gggtatactt acagaagagg aaaataaaag attagtagat
     6781 aagactataa taaatggtgg tagagtatca tacaaggata atccagatgg aactaagtct
     6841 gtatatgagt taaatataaa ctatttagat gccttattga ataaagatga agatatagat
     6901 gaggatatcc aaatacaaaa gatattatca gcacatgcat tattattatc atttataggt
     6961 gtaccagcta tatactatca ttctctatta ggctctaaaa actactatga aggagtagag
     7021 aaatcaggta taaacagaag aataaataga gaaaagttgg agtataatag aatttgttat
     7081 gaattagaac atgattccag aagaaataaa atatttactc aacttaaaaa tatgatagat
     7141 ataagaaagg gagagagtgc attttctcca tatgcaaatc aaaatgtatt ggattttgga
     7201 agagagatat ttgcactaga acgtgagaat aaagaaacta atgagaaggt agtttttata
     7261 actaatgtta cgcctaatga gaagattata gactgtggtt ttaatggtat aaatatatta
     7321 gaaaatacta atattgaagg aaagttaact ttaggtgcat ataaatttgc ttggataaaa
     7381 atcaaatagg agtttctgcc ttcagatatt acctaaggtg gttaaagcgg agtagtatgg
     7441 tatttattta aatatgaggt cattgattat ggtttagccc aaaatgttac atttaagttc
     7501 tgaaaagtaa tatttattgt ccaaaaagta gtatttaaag tctaaatagc cataaatata
     7561 tggcaaaaag atgtaaattg aagtaatata aaagtaagct caagtatacc aatgacaagt
     7621 ataccaatga aaggatgttt agactttgat taatataggg atatgtgatg atgaattaca
     7681 ttatagatta aagataaaag atatattaaa aaaggtatta agctcatata ccatggatta
     7741 taatatacat gaattttcat caggaaaaga gttgttatgt aactatccaa aaaatttaga
     7801 catattaatt attgatatac aaatggagat attaaatggt atagatacct caagaaaaat
     7861 acgagagttt gacgaaaatc tggagataat atttatgact tcatttcctg aatttatgca
     7921 agaagggtat gaagtcaaag catatagata catattgaaa ccaataaatg aaaaaaaaat
     7981 cacaaaaaat attttacctt gtatagatga gataatgaaa aaaaggaata attatttgac
     8041 tatcaaagtt aagaactatg tagatagaat taaaatagat tctattattt atatagaaac
     8101 agatagacct aatatagtga tttatacaca tgataataca tacactacaa aaatgagtat
     8161 ttctaaaata gaaaaaatat taagtgaatt tggatttttc agatgccata atagctatat
     8221 aattaattta aaattagtac aatctatgaa tggaaatagt gtagtaataa atggaaagag
     8281 tataccagta agtaaatata gagtgaaagg gttaaaatta gcaataacta atatattagg
     8341 agatataata tgttagaaac attaaatata aatctttggg atattattaa tataatcaca
     8401 ggctgtatag attggattat attcttttta gtcatgagta tattggggaa aaaaaatata
     8461 tctttcagaa aatatacaat ttatatgaca cttgtactcg tatctatgtc attggtcaat
     8521 tttatgagta tatttccaaa ttttaagatt atcatatgta tagtaatagg ttctttattt
     8581 tatattgctt catacaaaga gagtatatat aaatgtatat taattacctt attattttgg
     8641 ttaggattaa tgatggggga aggtttagct ataggtatag ttgtagttat aaatcaatta
     8701 aataatattg atataatact aaatgg
//
LOCUS       DAEMAE010000157         8666 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEMAE010000157 DAEMAE010000000
VERSION     DAEMAE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.159"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            308..1294
                     /locus_tag="KPH93_002754"
     CDS             308..1294
                     /locus_tag="KPH93_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1689552.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTSRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNVIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPRDISVAGFGGYDILSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1464..1610
                     /locus_tag="KPH93_002755"
                     /pseudo
     CDS             1464..1610
                     /locus_tag="KPH93_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1632..3113
                     /locus_tag="KPH93_002756"
     CDS             1632..3113
                     /locus_tag="KPH93_002756"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG1689553.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     VYSGNAIEVDGKLHMYYTGNIKYSTEDRYAYQNLAIMDKDGKMTKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNIKNIDEDF
                     GYMWECPDYINIDGKDVLIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFQIDIMK
                     EMEKGFDFYAPQTFTKESQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETIGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKVGNTVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYKYTKFKLKQGIEL"
     gene            3139..4110
                     /locus_tag="KPH93_002757"
     CDS             3139..4110
                     /locus_tag="KPH93_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG1689554.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSGFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNDCHSLHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKNAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAAHSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4366..4968)
                     /locus_tag="KPH93_002758"
     CDS             complement(4366..4968)
                     /locus_tag="KPH93_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1689555.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPGKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIKVDNC"
     gene            5249..6460
                     /locus_tag="KPH93_002759"
     CDS             5249..6460
                     /locus_tag="KPH93_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG1689556.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6528..6725
                     /locus_tag="KPH93_002760"
     CDS             6528..6725
                     /locus_tag="KPH93_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG1689557.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6759..7730
                     /locus_tag="KPH93_002761"
     CDS             6759..7730
                     /locus_tag="KPH93_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1689558.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNVLPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7917..8294
                     /locus_tag="KPH93_002762"
     CDS             7917..8294
                     /locus_tag="KPH93_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1689559.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3521 a          903 c         1378 g         2864 t
ORIGIN      
        1 aaaattattt tttaagtgat aattaagtat ttatcgataa ggctatggtt gtaaaaatca
       61 tagccttatt tttatattat aaataaatta gagtatttat caattcatag tagataaaat
      121 tgaaattaaa tataacaaca aaaaatgaaa attacactta atttaatgtt gaagactcaa
      181 ttatttggat gtggaatgga tagtacaagt agtatataaa aagtattgac tggacaaata
      241 actatatttt attttaaata tataatataa tgtaataaaa tatatcaaat gtagaaggtg
      301 ataacctatg aaaaaaataa caataaatga tattgctaac ttggcaggag tatctaaaag
      361 tactgtatca agatatctaa ataataaaga tatcagtgat tctactaaag agaaaataaa
      421 gacaataata gatgaatatg gatatgagcc gaatgcattt gctcaaagtt tgagagctaa
      481 aaaaacatac tttattggta ttataacacc gtgtttagat tcttttgtaa aatctaaaat
      541 tatgatggca atagatgaag aactcaaaga attaaaatat acatcactaa ttattaatac
      601 tagtagaaaa ataaggtcag aaatagatag tatatcaaaa ttagctagtc tgaaagttga
      661 tggaataata ctaattggta ccgaaataac taaagaacat aagaatgtaa ttgaaaagtt
      721 agatatacca atagttgtag ttggacaaaa agttgatggt atcaatagca tagtaaatga
      781 tgattatgga gcaggatata aaatgggtca atatattgct aataaaggtt acaaaaacat
      841 tgtatattta ggtgttgatg aaagtgatat atcagttggt ttaaatagaa aaaatggagt
      901 acttaatggg ttaaaagata aagggtatga tgcaaaagta ttttatactg actttgacca
      961 agaaacgtct atacaaagaa gtggagaaat gctagaaagt gaaaatccag atataataat
     1021 atgtgcaact gataatatag caatagcaac aatgaaagaa atcaataaga gaggtaaaaa
     1081 tataccacga gatatatctg tagctggatt tggtggatat gacatattgt ctattataag
     1141 ccctaaactt acaacaataa agtttgaaaa taaaaatgca ggtaaagtag cagcaaatac
     1201 gatagtaaac ttaatacaag aaagaaaaga accactatta aaggagatta aatttgaatt
     1261 aatagaaggt gaaagtacaa taaataaaaa ttaagataca cattacatat attagtaatg
     1321 tgtatttttt tactaaaaaa actataattc taaaaattag gaaaactttt ttgaaagaaa
     1381 tattgcatct aaaataaatt tatgataatc tttaattaaa ccggtttcga aaccggttta
     1441 attaaaaccg agagaatatc aaaatgagtg caaaaataaa tgattataac aaagtatcaa
     1501 agcaattatt agagtacata gaaaataaac aaaatataaa aggtgtagca cattgtgtaa
     1561 cgagattaag aattgtttta gatagtaata ataaagcaaa tataaaatta taaaagaaaa
     1621 ggaataaaaa tatgtacaaa gagcctaaat atagaaccat attagaatcg acaaaagaag
     1681 aattaaataa attaagaaat atatctttaa atgataaata taaaccacta tttcatatac
     1741 atccacaaca tggactactt aatgacccaa atggattagc atattataat ggtaagtacc
     1801 atgtatttta tcaatggtat ccatatgatg caactcatgg aatgaagcat tgggcttatg
     1861 taagttcaga tgattttgta aattggaata gagaagatgt agcattaata ccaatcgaaa
     1921 gttatgaatc tcatggagta tattcaggta atgcgataga ggtagatgga aaattgcata
     1981 tgtattatac agggaatata aaatatagta cagaagatag atatgcatac caaaacttag
     2041 ctataatgga caaggatggt aaaatgacta aatatgaaaa taatccaata gtaagtgaaa
     2101 taccaaaagg atatacagga catgtaagag acccaaaagt atttaaaaga aaggataaat
     2161 actttatgct acttggagca caaacaagtg ataaaaaagg agttataata gtatatgaat
     2221 ctaaaaactc tatagattgg aattttaaag gagaattaaa tataaaaaat atagatgaag
     2281 attttggtta catgtgggaa tgtccagatt atattaatat agatggaaaa gatgtattaa
     2341 tattttcacc acaaggagta gagcctaaag gatttgatta tcaaaatata tataatgtag
     2401 tatatgctat tggtaatatg gatttagata atttaacatt tcaaatagat ataatgaaag
     2461 aaatggaaaa aggatttgat ttctatgctc cacaaacatt tacaaaggaa tcacaaataa
     2521 tactatttgc atgggctgga atgggagaag ttttataccc cacagataaa aacaaatggg
     2581 cacattgtct aactgttcca agaaaattaa atataaaaaa taataagcta ttgcaaatgc
     2641 ctgtagatga attaattaaa ttaagatatg atgaaacaat tgggcaaaat actataaaga
     2701 ataatattaa tattatagaa aatgatgaaa atgtatatga gcttaacatt aatataaaaa
     2761 atattgacag caataaattt gggctggaat tattttcatc acaagatgaa ggtgtaaaat
     2821 tagaatttaa caaagtagga aacactgtaa ctttagacag aagcaacttt aaaaaagttt
     2881 tcggtgttga atatggaaca aatagaaaag aatatataaa tattgatgaa aatactaata
     2941 tcaaagtact agcggataga agtattttag aaatttttat aaatgatgga gaagtagtat
     3001 ttactagtag aatatttgct aaagaaaatt ctaatcaaat aagggtttat agtgataaaa
     3061 tagtagagta taagtatact aaatttaaat taaagcaagg tattgaatta taaaaataca
     3121 ttataagggg tagaaaagat gaaaaaagta attagtatag gagaagcttt aatagacttt
     3181 atacctaatc aaaatggttg taaattaaaa aatgtatcag gatttagaag agtagcagga
     3241 ggagctccag caaatgttag tgctgttgta gcaaagcttg gaggaaaatc aagttttata
     3301 tctaagttag gaaaagatgc atttggtgat tatataatag atgtattaaa tgaagtaaat
     3361 gtaaatactg attatgtatt gagaacaagt aaagcaaata cgggacttgc ttttgtatca
     3421 ttaaaagaag atggtaatcg tgatttctca ttttatagaa atccaagtgc agatatgctt
     3481 ttagaagctg atgaagttaa aaaagagtgg tttaatgatt gccatagcct acatttttgt
     3541 tcggtagatt taatagatag tccaatgaaa ttagcacata aaaaagcaat agaatacgca
     3601 cttgaaagta atagtataat aagttttgac cctaatataa gattaccttt atgggatagt
     3661 gagcaatctt gtaaaaatgc aatatcagaa tttttacctt ttgctcacat tgttaagata
     3721 tctgatgaag aattagaatt tataacagga gaaaatgata tagaaaaatc attacataaa
     3781 ctctttgtgg ggaatgtgga attagtatta tatacaaaag gaaaagatgg agtagatgcg
     3841 tacactaaaa aagtaaaagg tatgtgtaaa ggagttaaag taaatgcaat agatacaacc
     3901 ggagcaggag attcttatat aggttcattt ttatacacat tgttgtataa acaaataaca
     3961 ttgaatgata taaaagaaat gaaacaagaa acgttaaatg agtatttaga attttctaat
     4021 tattatgcgg ctcatagtac gactattaaa ggtgcgataa gttcatatgc aacaaaggaa
     4081 gaaataactg aatttataaa aaatttatag taggaatgag taataggttt tgaaggaggt
     4141 aacatgtaaa gagtttttaa ggatgcttac tatgaaaata attaatatat aaaaagtaat
     4201 aaagaggctt tctcaaaata gagataagcc tctttatcat tgttgaaatg ataaaaaata
     4261 ttttaattat tgttactttt tagaagaata tatatcttat tttaaataag caaattatgt
     4321 aagatttatt tatattatca atactttact tggagtatta tgtctttagc agttatctac
     4381 tttaattgaa ttatctaaaa tataataaat ttgttttgca aattgacgag aatgttcttc
     4441 tttttcttta ttcataaaat taggtttacc taaatcacgt tcaacaagca ttcctatata
     4501 ttttaactgt gaatgcttac agtagcgctt cataccattt tcccaaatat cagcaccctt
     4561 tccaggtcta tatccacatg ttgtaataag tgctactggt tttcctgccc atagagcggg
     4621 acctttctca ttaccatagt atttattcat tccatacacc aatctgtcaa gtagtgcttt
     4681 cattggagaa gtgcagtacc aagaataaat tggagtagca aaaacaatta aatcacacat
     4741 aagaattttc ttgaaaattt tctgaacatc atctttacaa taacatccaa aatcaaataa
     4801 gttatcctga cattttttac aagcaatgca tggctctatt ttacaatcat ataaccatat
     4861 tacttcaata tttgaattat aattttctaa ttcctcaata aaaggtttta aaatagcagc
     4921 agtattacct tttttcttag gacttcccat aagaatgcat acttccatat tgatacctcc
     4981 acagtatttt aaaatagttt ttagtgctta atatagtttt aggatactat gagaatacgt
     5041 taacaacaat acactttgta tatttgtaat aaaattgaaa caaattgttt taccaataaa
     5101 agttggaatg tatctgtaat taaaaatatt ttaggtatat ttttacatat atgtgtgtag
     5161 ttaactatgg taatttattg taaaattatc ttataggatt ataattgctt aatttatgat
     5221 tatatttaaa atcttggagg taaaaaatat gtactcaaat gaagactata aatttataga
     5281 agaaaatgca ccatgttata tatatgatga gactaatata attgaaaaat gtaaggaatt
     5341 gaaaaataat attttaggtt ttgatttttt atattctata aaaacaaatc catataacaa
     5401 aattgtaaaa agcattgcag agcaaggttt tggagcagat gcttcttcaa ctaatgaagt
     5461 tatgatttca tcaaaatgtg gagtttctaa agatgatatt tattattcat caccaggcaa
     5521 aacagagatt gatatagaaa ctgttttaga taaatgcata attattgcag atagtattac
     5581 agaaatagaa cttataaata agatatctaa aaagcataat cagaaagtta aaataggaat
     5641 aagaataaat cctgatttta ctatggatga tgatagtgct aaatcaagca aatttggtat
     5701 agatatagaa gatatgagca gtgtttttaa gatgttaagt atgtgtgatt atgtatctgt
     5761 agttgggatt catatacata ttaaatctca ggtattggat tttgaaaagt taggacatta
     5821 ttatgaaaaa tgttttttta tagctttaaa tttaaataaa agtaagtata ttaatattga
     5881 atttataaat tttggtagtg gacttggagt agcttatgat aatcttagag aaaatccact
     5941 taatcttaaa gagctttcaa aattaactca aaagattgct tttactaatt caaacacact
     6001 gaaatcaaga cttcttattg aatcaggacg tttcatagtt tgtaaatcag gtaattatta
     6061 tgttaaaatt gtagatatta aagtatcaag agggaaaaaa tatcttattg taaaaaatgg
     6121 acttaatgga tttatgagac ctattatttc taatttaatt gaaaatatta cttgtggagt
     6181 agaattagaa ggtcaagagc cattatatac atcaaatcat gccttttctc tttcagtatt
     6241 aaataattta aaagaagaag agtatgtaaa tgttgttggg aatctctgta catcattaga
     6301 tgtaattgca aataatgtta aattgaaaaa agcaaacata ggagatttga tttcaattag
     6361 taatgctgga agttactcct attctttatc accactatta ttttctagtt atgaatttcc
     6421 taaacaatat tataaaagaa aagatggata tattgagtaa gcaatagcat aaattaataa
     6481 acttagtatt attgaaaaaa tttaaaatgc agggggtgta attctatatg gaattgtgga
     6541 aaagaaattt atttgtatgt tggataggta tgttttttag ttctataggg atgagccaaa
     6601 tagcacctat acttccatta tatattaaac agttgggggt aactgatgtt agtttaatac
     6661 aacagtactc aggtataatt tttggatgta cttttgtagt agcagcattc ttttcgccaa
     6721 tttgagggaa agctgctgat aaatatggaa gaaaatcaat gcttttacgt gcaagtattg
     6781 ggatgggaat agttatattt accatggcgt ttgttcaaaa tgtttatcag ttattaggat
     6841 taagaatact tcaaggagtt tttacaggtt atgcaacagc atgtactact cttattgcaa
     6901 cacagacaga taaaaatcat tcaggatggg ctttaggtac tcttgcaact gcatcaacta
     6961 ctggctcatt aattggtcca acagtaggag gatatataga atcaatctta ggtcttaaaa
     7021 gtacatttat tataacagga ggattgttgt ttgtatcatt tatcatctct attctatttg
     7081 tagtagataa ttttaaacct aaagaaataa aagaatcaat ttctatacct aaagaacaat
     7141 taaatgtatt gccaaataaa tttttaattg catctatttt tgtaaccaca ttcattactc
     7201 aactagcttt atattctata gaaccaattg taaccatata tatatcacaa cttacaaatt
     7261 ttgcatcaaa tgtagcttta atagctggat tgacattttc tgcatcagga ttagcaaatt
     7321 tattagcagc tcaaaaactt ggaaaaatgt cagataaaat aggtcctcaa aaagtattgt
     7381 taatttcact tttatgggct ggaattatat ttataccaca agcttttgta aaaactgcat
     7441 ggcaactgat gttgttgaga tttttattag ggttgtcagt tgcagggctt aatccttctg
     7501 ttaacagctt gcttaaaaaa attgcaccag aagaatatgt gggaaaaata tttggatata
     7561 atgcatctgc ccagtatata ggctgttcat caggagcatt tttaggggga caaatatcag
     7621 cacatttagg tattagaaca gtattttttt caacaagttt attgttgttt atgaatgcac
     7681 tttggatgta taaatttact ggattgttta ttaaagataa gactaaataa tcatactatt
     7741 tacatttcta attttatgat gtaaaataaa tatttcttat ttaatttatc atagaattat
     7801 acaaaaaata accctatggt aaaaattgga aaatattatt atcgacaaaa tctttgactt
     7861 aaaaactata ttgcgatagt attaatttaa taaaagtttg agaggggcgt aaggttatga
     7921 caatttcaaa aataccacaa gcagaattaa aagttatgaa gtttatttgg gaaaaaaatg
     7981 atacagtaac atcaaaagaa actattgaag ctatggaacg aaaatatggt tggaaacaaa
     8041 caactacatt gacactttta tcaagacttg ttaaaaaaca ttttttaagt gcagaaaaaa
     8101 tagatagata cactcattat acaatagttg ttggacacaa ggagtattta agtgttgaga
     8161 caaaagattt tttaagcaat atacacgaca attctttaca gagcttgatt tcagcactac
     8221 atgatgatga agttgttgat aaagatatgt tggatttatt tgaaagtcat tttaaaaatt
     8281 tagaagaaga gtaattatat caggatagaa ctctaagaat ttaattaatc tatggctgaa
     8341 aaccataaat ggtattattt aatcagctac tcactattca taaaataata ttaaataggt
     8401 tctaattagt aaaatgccta aaataattgg aatagggagt atcaagttaa atgataccac
     8461 ttatttaagt aagaaatgat ttttaaaatt taaaataatt ttataacttg tcaatattca
     8521 ttaatctaaa gtattatttc tatttaacca tatcaaaata tatttgacta tttttaatta
     8581 ttacaacata aatctgtctt ttgttattat aggtaaacta aaagtaagaa atgatttcta
     8641 tatttttaaa ctttattatt ggtaac
//
LOCUS       DAEMAE010000158         8633 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEMAE010000158 DAEMAE010000000
VERSION     DAEMAE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.160"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            305..1261
                     /locus_tag="KPH93_002763"
     CDS             305..1261
                     /locus_tag="KPH93_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG1689560.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            1438..1593
                     /locus_tag="KPH93_002764"
     CDS             1438..1593
                     /locus_tag="KPH93_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689561.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1723..2736
                     /gene="aroF"
                     /locus_tag="KPH93_002765"
     CDS             1723..2736
                     /gene="aroF"
                     /locus_tag="KPH93_002765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG1689562.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2758..3822
                     /locus_tag="KPH93_002766"
     CDS             2758..3822
                     /locus_tag="KPH93_002766"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG1689563.1"
                     /translation="MGEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLVRGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKDREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYGIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3822..5135
                     /gene="aroA"
                     /locus_tag="KPH93_002767"
     CDS             3822..5135
                     /gene="aroA"
                     /locus_tag="KPH93_002767"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG1689564.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKREDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            5113..6192
                     /gene="aroC"
                     /locus_tag="KPH93_002768"
     CDS             5113..6192
                     /gene="aroC"
                     /locus_tag="KPH93_002768"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG1689565.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGKTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQELSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIVECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGKQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSINRQQNTVNIKDKKDDILSIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            6195..7391
                     /gene="pheA"
                     /locus_tag="KPH93_002769"
     CDS             6195..7391
                     /gene="pheA"
                     /locus_tag="KPH93_002769"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG1689566.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETIQFFNSIMEISRGLQKRLMDNGSEQKIEFCLDS
                     SNKKFDLSNVNKYKSLKYELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEIDYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVKKLQDKTKAAIASKK
                     AANIYGLEIISPCINDITNNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7438..8247
                     /gene="aroE"
                     /locus_tag="KPH93_002770"
     CDS             7438..8247
                     /gene="aroE"
                     /locus_tag="KPH93_002770"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG1689567.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDSAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMASIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRNKDDKKLLNS
                     KAILIDYEELKCIKGDIILNATPVGMYPNVGISPVSKSIIQNFDTLIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDIKIDNSILDAIYNELKLEFL"
     gene            8259..>8633
                     /locus_tag="KPH93_002771"
     CDS             8259..>8633
                     /locus_tag="KPH93_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG1689568.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKN"
BASE COUNT         3522 a          847 c         1519 g         2745 t
ORIGIN      
        1 tatagtttag ttaaaataag aatgaagtgt atgtgctgtt gtcagtttag cacatgcatt
       61 ttatttttta gcttttttta gtatcaataa ttttttagta ttaataatat gaaatatgtt
      121 ttaaaataag tatatagtgt aatctgtaat aaaaactttg gttaaataat aaatgtactt
      181 aacacttacc ttataataaa tttaatataa atattaaata ataattatta aaatagtgta
      241 aaataattac agtaaggatt ataaaaaatt tttagaatgt aaactgaaat ttaggagtag
      301 atgtatgtat atactgaatt taaatacaag agagactatt gaagatttta gggataaatt
      361 ttatgtagca gaaaattctt atcttatatt aagtgctcca aaaaatttaa aattgcttaa
      421 ggaaactttg gatatagatg aaataacttt taatgattgt cttaaatttg atgaaataac
      481 caaacttgat ttatttgata attacgattt tttaagccta aatacatttg aattaagaga
      541 tggagaagct gttatagaag aggtaaatat gtatttatca gataatttta tattggttgt
      601 ggtgaatgaa gaacactttt tatttgaatt tgtaaaaaat attattttga agaactctca
      661 acttgaaaaa aatccagtta ttaatctatt taagataaat tacttgattc ttagagaagt
      721 tataaaaaat ggttttgagt ctttggaaaa agttgaagag ttaattcttc aaatagaaga
      781 tgaaatgatg gacaacatta ataagaatca tgtatctaga ataagtgatg taagaggatt
      841 aacaaggatt atagttaaga atactagacc tcttttgtat attggggata gaatagttaa
      901 ggaaaatata agatatttaa agtattcaaa tgtaaaaaaa tataatttag aaaattttca
      961 aggtatagat tttggaattg acaaattata caatttcgca ttgtcaacac gagagttagc
     1021 agataaatta ttagatattt attcttccag agtaggtgag aaaacaaata acttaattac
     1081 aaaattaacc ttgttaacag ccatatcagc accacttaca attataactg gtatatatgg
     1141 aatgaacttt agatatatgc cagaattaaa ctggatttat ggatatccag caactttatt
     1201 ttttatgtta tgtataattt ttgttggaat tataatattt aaaataaaaa aattattgta
     1261 aattacagat gatgtttaat tgattttata aaaatacatc aattgagaag ctgatttaag
     1321 taaataaaaa aatattttaa attgaaaata tttatagaaa atattgacaa ttatattttc
     1381 tttgttataa ttattaaaca agaagaataa taaattataa aaatggagga taatactatg
     1441 aaaggaatat taaaagaact aacagcaaaa ctttatagct ttttttactt tatgttctgg
     1501 ggcttttatt ttagcgctgg ctatttcttt tacaaaaata tatgtacttc ctttctggtg
     1561 aggccttatt ttagtgattt tgagcataag taaattttat aaaaatgatt attttatata
     1621 tttatataat atttagtgct tgatagatta ttttagagaa ctcattagtg agttctcttt
     1681 tttattttaa atataaaatt ataatattgg gaggtagtaa aaatgatagt agtattaaaa
     1741 atgggagcag ataaaaatga agttaaaaaa ttaattgagg ctattgggag agaaggagta
     1801 gaagttaatc ccatagatgg aacagaatta actgttttag gtttagttgg agatactagt
     1861 aaaatagatg ccaaaagaat agaagcaaat aagatagttg aaaaagttat gcatgtagta
     1921 gaacctttta aaaaggccaa tagaaaattt catccagaac cttcaatcat aaatgttaat
     1981 ggaatggaaa ttggttcaaa gaaaattgct atgatagctg ggccttgttc tgttgaaaca
     2041 gaagaccaaa tagtatctat agctaaagat gtgaaaaaat caggtgcagg atttttgaga
     2101 ggtggagcat tcaaaccaag aacatcacca tacgctttcc aaggattgaa atatgatgga
     2161 ttggatttat taaaaaaagc aaaagaaaaa acagggcttc ctatagttac agaaataatg
     2221 tcaacacaag atatagatat atttgaagaa aatgtagatg taattcaagt tggagctaga
     2281 aatatgcaaa acttcgattt attaaaagaa ttaggtaaaa caaataaaac aatactttta
     2341 aaaagaggat tatctgctac aattgaagag tggctaatgt cagcagagta tataatggct
     2401 ggaggaaatg aaaatgtagt tctttgtgaa agaggtataa gaacttttga aacatatact
     2461 agaaatactt tggatttaag tgcaatttta gctgtgaaga aacttagtca tttaccagtt
     2521 atagttgacc caagtcatgc agcaggtaaa tcatggatgg ttgactcttt atctaaggca
     2581 gcaattgcag tcggagcaga tggtctaata atagaagttc ataatgaccc agcacatgca
     2641 ctttgtgatg gaaaacaatc gattaagcca aatgaatatg atgaattgat aagtgaatta
     2701 aagactatcg catcagcagt tggcagagaa atataatcta taaaaggatg gttaaacgtg
     2761 ggagaaaaac tattagtaaa tttaaaagaa aatagctaca atatatttat taaaaaaaat
     2821 atactaaaga atataggaga agaaataaaa aaaatatatt ttggaaataa gatttttatt
     2881 gtaactgatg aaaatatagc aaaattatac ttggataaag taaaaaataa tttaagtgaa
     2941 tctggatttg aaattaaatc tttaatatta aaatctggtg aagaaacaaa atcatttaat
     3001 actcttccta aaatatacga tagtttactt gactttaagt taactagaaa agatttgatt
     3061 attactttag gaggaggggt aataggtgat ttaggtggat ttgcagcagc aactttttta
     3121 agaggtataa gttttattca aattccaaca tctttattag cacaagttga tagttctgta
     3181 ggtggaaaag ttggagttga ccttgtgaga ggtaaaaatt tagtgggaag cttttaccaa
     3241 ccaaaggctg tgtttatcga cccaaatgta ttattatcac ttccagaaaa attttataga
     3301 gatggaatgg cagaagttat aaaatatgga tgtataaaag acaaaacttt ttttaataaa
     3361 cttttaagtt taaaagatag agaagaagta atgcaacata tagaatacat tatacataaa
     3421 tgttgcttta ttaaaaaaac tgtagttgaa gctgatgaaa aagactttgg agatagaatg
     3481 cttctaaact ttggtcatac attaggtcat gctattgaga aatactacaa ttttacgggt
     3541 tatacccatg gagaagctgt tgccatagga atgtataaga taactttaga aagtgaaaag
     3601 ataggaatta caaaaaaagg tacatcagaa cttataaaat caattttaat gaactataat
     3661 ttgccttatg ggattgaatt aagagataat aaatctatag tagatgctat atctcttgat
     3721 aaaaaaaatc taggtgatac attaaaaata atacttttaa aagatattgg agagagtatt
     3781 atatataata ctacttctaa attttttcag gaggtaagat aatgggaagt ctaaaaatat
     3841 atccaagtaa attatctgga gatgtaaaaa taccaccatc aaaaagtatg gcacatagag
     3901 cagttatttg ttcatctcta agcaatggaa aaagtagaat ttctaatata gatttttctg
     3961 atgatataat agctactata agagcaatga catcattagg agctattata gaaaagagag
     4021 aagatatttt agaaatttcg ggaatttttt ctaaagaagg tattttaaat agagagaatc
     4081 agttgaatca acctaaattg acaatagatt gtaatgaatc aggctctaca ttgagatttt
     4141 tagtacctat atctttagca tttgatggtg taaaaaggtt tataggtaga ggtaatcttg
     4201 gaaaaagacc acttgatact tattatgaga tatttgacag acagaatata aaatatagct
     4261 acaaagaaaa tcagttagat ttgattatat caggtaagct taaaccagat gaatttagag
     4321 taaaaggaaa tataagttct cagtttatca caggtctatt gtttatattg cctacattag
     4381 agagtgattc aaagataata attactactg agctagaatc taaaggatat ttagatttaa
     4441 ctctttcaac gataaaagat tttggggtag aaattataaa caataactat aaagaattta
     4501 ttataaaagg aaatcaaacg tataaagcga gagattataa agtagaagga gattattctc
     4561 aaggagcatt ttatttatca gcagatgcta taggagaaga tattagcata ttagacctta
     4621 aagaagattc tcttcaaggg gatagtgaag tagttgaaat tttatctagg atgggaatgg
     4681 aaatattaag agaaggcaac aaaataaaag gaataacaaa tggtttaaat agtactttga
     4741 tagatgcatc acaatgtcca gatataatac ctgtattatc tgtagtagca tccttaagta
     4801 tagggaagac tacaataatt aatgctggta ggcttagaat taaagaatgt gatagacttc
     4861 atgcaataaa tgttgagtta agtaaacttg gagcaaatat agaggagaaa gaggatagtt
     4921 tgataatcga aggagtaagc aagttaaatg gtggagtaga agtgtggagt cacaaagacc
     4981 atagaattgc tatgacactt gcaattgctt cttgtagatg tgataaacca ataatattaa
     5041 aagattttga gtgtgtatca aaatcttatc cacatttttt taaagatttt aagatgttag
     5101 gaggtcgaat tgatgagtgg aatatgggga aataatttaa aagtatcaat ttttggagaa
     5161 tctcatggaa atgctatagg tataaatata gatggtcttc cttcgggaat tgaacttgac
     5221 cttgataaga ttgataaaga aatgaagaga agagcacctg gaaaaaattc tatatctact
     5281 tcaagaaatg aaagtgatat accagaaatt ctaagtggat attttaatgg aaaaactaca
     5341 ggaacgccat tatgtgcaat cataagaaat agtgatactc gttcaaaaga ttatggagag
     5401 cttaaaaatt taatgagacc aggacatgca gatttcacag gtaatgtgag atattctgga
     5461 tttaatgact atagaggggg aggtcatttt tcaggtagaa taacagctcc attagtattt
     5521 tgtggggcta tatgtaaaca aattttatca caaaaaggaa tagagatagg agcacacata
     5581 aaaaaaatta aaaatataga agatatgagt tttgattatg taaatatttc aaaacaagag
     5641 ttatcaaatt tgcaaacttt ggaactacct ttgcttgatt tatcaaagga agaagccatg
     5701 aaaaatacga ttatagatgc aaaaaatcaa ggagattcag ttggtggtat agttgagtgt
     5761 gcagtagtag gtattaatgt agggcttgga aatccatttt ttgactctgt tgaatctacc
     5821 ttatcacatt tattgttttc tgttcctgct gtaaaaggtg tagagtttgg cttaggtttt
     5881 gaacttgcag atatgtatgg ttctcaaagt aatgatgaaa tgtattatga aggaaagcaa
     5941 gttaagagca aaacaaataa caatggaggg attataggag ggattactac tggaatgcca
     6001 ataattttca aggtggcaat taagccaact ccatcaatta acagacaaca aaacacagtt
     6061 aatataaaag ataaaaagga cgatatttta tctattaaag gcagacatga cccttgtata
     6121 gttcaaaggg ctatacctgt aatagaagca gtaacggcca ttggaatttt tgatttgatg
     6181 aaaggacgat aataatggag gatttaacga gatatagaaa tgagattgac aatatagata
     6241 aagagattat tcaattattt gaaaagagaa tgaatacagt tcttgaaatt gccagacata
     6301 aaatgaaaaa taacactact atacttcaaa aagatagaga agaaaaagta ctaagtagag
     6361 ccgtagataa tttagataat aaagcatatt cacaagaaac gatacagttt tttaattcta
     6421 taatggaaat tagtagagga ttacaaaaaa gattaatgga taatggtagt gagcagaaaa
     6481 tcgagttttg tttagattca tcaaataaga aatttgattt gtcaaatgta aataaatata
     6541 agtctttaaa atatgaatta aataaaaaaa atatcctagt aggttttcca ggaaaatcag
     6601 gttcatttac agaagaagca ctaaataaat tcttcaacaa aaaaatatca aaaaaacaat
     6661 ttaaagaatt tgaagatgtt ttcatagcac ttaaaaataa agaaatagac tatggaataa
     6721 tacccataga aaattcatct acaggagcta tctctgaaac ttatgacctt ttaagaaagt
     6781 atggttttta tatagttgga gaagaatgca taaaaataga ccaaaattta atagggataa
     6841 aagatacaaa acttgaagat ataaaagaaa tatattctca cccacaaggt ataggacaat
     6901 gtagtgaatt tttaaaacaa aatagtacat ggaaattgat accatttaat aatacagcaa
     6961 caagtgccga acttgtaaaa aaacttcaag ataaaacaaa agcagcaatt gcaagcaaaa
     7021 aagctgcaaa tatctatgga ttagaaataa taagtccttg tataaatgat attacaaaca
     7081 actatacaag atttgtagta attagtaatc aaatacacat tgaagaagaa tcaaacaaaa
     7141 tgagtgtagt attttctgtt gaacatgaag caggaaaact ttataaagta cttggatact
     7201 ttgctgaaaa taatataaat atgacaaaaa tagaatcaag acctatgaaa aatgcctcat
     7261 ggagatattt cttttatata gattttgagt gttctatata caattctaaa gtgtacgatt
     7321 tattagaatt gatagaacat aatacagcat attttaaatt tatgggagta tataaaaata
     7381 aaactgaata gataagataa atttttattt aatttataat tattaggagg tataagtatg
     7441 aattttttcg gtctagtagg tgaaaaactc tctcacagtg tttcacctca aatccacaag
     7501 agagtattcg agatattgaa tattgaaagt gcttataaaa actttgaaat ttcaaaagaa
     7561 gatatatcta agctagacag tgctataaaa ttattaggaa tacaaggagt aaatgttact
     7621 gtaccatata aggagcgtat tatgaaatat cttgatttta tatcgccaga ggcaaaacgt
     7681 ataggagcag ttaatacaat tttactcaga gaaaatatgt tatatggtta taatacagat
     7741 tactttggcc ttgatagtat gtttaaaatg gctagtattg atgttcaagg aaaagtagca
     7801 gttatattag gaactggagg tgcttctaaa gctgctctta catattttat cgattctggt
     7861 atagaaaagc tttatgtttc aactagaaat aaagatgata aaaaattatt aaatagtaaa
     7921 gctatattaa tagattatga agaacttaaa tgtataaaag gagatataat attaaatgct
     7981 acaccagtag gtatgtaccc taatgtagga atttcaccag tgtcaaaatc tattatacaa
     8041 aattttgata cattaataga tttaatttac aatcctggag aaacagagtt tttaagaatt
     8101 ggtaatagta tgggcaaaaa aacctgtgat ggattatata tgctggtagg gcaagcaata
     8161 aaatctcagg aaatttggca agatattaaa atagataata gtattttaga tgctatatat
     8221 aatgaactaa aattagaatt tttgtaaggg agattgttat gataaataaa acaaaagaaa
     8281 aattgatatt aataggtatg ccaggtagtg gaaagactac aattggtaaa cttttggcca
     8341 aagaatataa ttgtagtttc tgtgatatgg atgattatat aattcaaata tctcaaaaaa
     8401 gtatagcaga gttgttttca gaaggagaag atatttttag gaactatgag actcaagcat
     8461 gtagagaact ttctataagt gataaaacag tgatatctac aggtggaggt gtaataaaaa
     8521 aggatgttaa tatggaaata ttaaaagaaa caggtataat aatttttatt gatagaccta
     8581 tacaaaagat tttggaagat ataaatatta attcaagacc attattaaaa aac
//
LOCUS       DAEMAE010000159         8589 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEMAE010000159 DAEMAE010000000
VERSION     DAEMAE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.161"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..2073
                     /locus_tag="KPH93_002772"
     CDS             37..2073
                     /locus_tag="KPH93_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG1689569.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIKALKDDFDVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKDGGFYSAQDADSEGEEGKFYI
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSEKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLENDIYLEYSNKCLDFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNENCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDSRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIISFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(2266..2832)
                     /locus_tag="KPH93_002773"
     CDS             complement(2266..2832)
                     /locus_tag="KPH93_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1689570.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVVPSPHDKRAINVRV
                     TDLGKKVMVTCNRTGINFMADVFHEFTKDELETLWNLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            3119..4057
                     /locus_tag="KPH93_002774"
     CDS             3119..4057
                     /locus_tag="KPH93_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1689571.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKTKAVIQIAHGMAETAQ
                     RYETFAKVLTENGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYGNNLSGLILCGSNGKQGIILNFAHMIINREI
                     KKYGRRSRSNKINDLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINKEEVFEDIICWLNNKIEILR"
     gene            4438..5172
                     /locus_tag="KPH93_002775"
     CDS             4438..5172
                     /locus_tag="KPH93_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689572.1"
                     /translation="MNKILRVKNIKKYYKSRGNVTKAIDNISFEVERGEYIGIMGASG
                     SGKTTLLNCISTIDKTTEGNIYIGDKDITTMDEDELSKFRREELGFIFQDFNLLDTLT
                     AHDNIALSLSINGVNSKEIDNKIKKIANKLGVHEILNKYPYEISGGQKQRIACARAII
                     TNPSLILADEPTGALDSKSAKLFLENLEVLNHELKATILMVTHDAFTASYCKRILFIK
                     DGKIFNELVRGDNTRQEFFDRIIKVI"
     gene            5195..7324
                     /locus_tag="KPH93_002776"
     CDS             5195..7324
                     /locus_tag="KPH93_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689573.1"
                     /translation="MFFRKNKSRRVRNKRKNNYVSKVSSVSSESFKKLALNNVKKSLK
                     NYLIYFITLVFGVIILYTFNSLDNDIAILSNNALLSSSISFVRGIVSIFSILVCIIFA
                     FLIIYANNYLMKQRKKEFGIYSILGMDRRDINKLMFREMMIIGIFSLIIGIVFGVFIS
                     KGLSIFALKMLGISSSGFSFSILTTIKTIVFFGIVLLLVNKFNKKTIKKYKLIDLLNA
                     NKKNEVSVAKGKKSNLILFFLSIVSIVLAYIIPLKMKISTFVLCICLILILVGIYLFF
                     MSVSDFIMLKIKKHRFIYYKNLNPFTVSQISSQIKTMSMSITMICMLLFSSLIVIPFG
                     ISFGTYLIDDLQEATPYDVTLSKLFMNGDNIVEYSTKKSDINSKIPSLKDELIANDFP
                     LISFSKSTSDLRIYNLKNISQYDFITNDKDKTIKLKDNKLHIVSISDYNSTRKQSGLK
                     EINLKDNEFAINCASENYRQFYEKYIKSQPQNYITLNGVKLKLNQTSLYTNSYNTNTV
                     PLDLGTIIVPDAVIKDLNPIMITLNIDYKERSSNLENKFMDSYYKFEEKIDNSEDYSI
                     LYKSVIDGEKRALNTSFSFVAIYIGIILLISAGAILALQQLIESTVNKERFKLLSKLG
                     VNKKDMKKAIFIQISILFAVPLILALINALFISKVLFVIMPFIAETGVITNMLLTLGL
                     IIIVYGIYFIASLLESLNIVNNSKEII"
     gene            7541..>8589
                     /locus_tag="KPH93_002777"
     CDS             7541..>8589
                     /locus_tag="KPH93_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689574.1"
                     /translation="MQIKIGKVIQHLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEC
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNNRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNMSMDCKGIINVYKNQYKELKKKLENRNSNKNAIERKMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMR"
BASE COUNT         3428 a          843 c         1386 g         2932 t
ORIGIN      
        1 caaaatatat aataaaataa caaatgaggt ggtattttgg acagtaatag aaaacctaat
       61 aatctaataa atgaaaaatc tccatatctt ttacagcatg catataatcc tataaactgg
      121 tatagttgga atgatgaggc ttttaaaaaa gcaaaagaag aggataagcc aatattttta
      181 agtgtaggat attcaacatg tcattggtgc catgttatgg aaaaagaatc ttttgaagat
      241 gaagaagttg ctgaaataat gaatagaaac tttgtggcga taaaagtaga taaagaggaa
      301 agacctgatg tagatagcgt gtatatgaca gtttgccaag ccatgacagg tagtggtgga
      361 tggccaatga ccataataat gacaccagat aaaaaacctt tttttgcagg aacatatttc
      421 ccaaaatatt caaggtataa taggcctgga gttatagatt tattagagaa tgtatctgaa
      481 aaatggaata caagcaggga tatattaata aaatctggag atgaaataat aaaagctctt
      541 aaggatgatt ttgatgtaaa aaatactgag ggcgatttat caaaagagat gttgagttca
      601 tcagttagag tatttaaggc tatttatgac gaaaagtatg gtggttttgg taatgctcct
      661 aaatttccaa gtcctcagaa tcttatgttt ttgatgaaat attatagtat agaaaaagat
      721 aaagatgtgt taaaaatggt ggaaaaaact ttagatggaa tgtatagagg aggcttgttt
      781 gatcatattg gatttgggtt ttcaagatac tctactgata aaaagtggtt ggctcctcat
      841 tttgaaaaaa tgctatatga taatgctatg cttactatag cttttttaga tgcgtataaa
      901 ataacaaaga aagagttata taaagaaatt gctataaaaa ctatagatta tgtagttaga
      961 gagatgaagg ataaagacgg tgggttttat tcagcacagg atgctgatag tgaaggtgaa
     1021 gaaggtaaat tttatatatt taatcctctt gagataattg aagttttggg tgaagaagat
     1081 ggaactttct ttaataatta ttttgatata actagtagtg gaaattttga aggcaaaagt
     1141 attcctaatc ttataaaaaa taaagaatat gagagacata atgaaaagat agctgacctt
     1201 agtgaaaagg tatttgaata tagaaaagaa agaacatctt tgcataaaga tgacaagata
     1261 cttacatcat ggaatgcatt gatgatagtt gctttaacaa aagcatatag tactttagaa
     1321 aatgatatat atttagagta ttcaaataag tgtcttgatt ttataaataa taatcttgta
     1381 aatgagtcag gtagattatt agctagatat agagatggaa gttccgatta tttagcatat
     1441 ttagatgatt atgctttttt aatctgggcg tatatagagc tttatgagtc aacatttaat
     1501 atgaaatatt tagaaaaggc tttaaatttg aatgaaaact gtatcaacct tttttgggat
     1561 tatgaaaaaa gtgggtttta tatatatggt aaagatagtg aaaatttaat agcaagacca
     1621 aaagatttgt atgatggagc tattccatca ggaaattctg ttcaattata caatcttata
     1681 agacttgcga aaattactgg agatagtaga cttgaagaaa tgtcatataa acaattaaaa
     1741 ctatatgtgg ataatgtaaa gagttctcca actggctaca gcttttatat gctttcattg
     1801 atgtttgaac tttattctac taaggaaatt atatgtattt ttaaggaaga ttctgattta
     1861 attgcattta aagaattgat aagtgaaaat tttataccaa atgcaacttt tttagcaaag
     1921 aaatacaatg aagaaaacac tattataagt tttttaaata actatagatt gaaggatgat
     1981 aagactagtt attatgtgtg tcaaagtaat agttgtagcc aaccaattaa tgatttacaa
     2041 aaattaaaag atatgatttt aggagtagaa taaccataat agtgaaaaag gagatgcttt
     2101 aaaataaaag tctaatcatc tccttttttg tacaaaaaac agaaaaagag ttcctaagaa
     2161 cacctaatct atgtaatgat tgttttgtta aaaaataaaa atgacattat agttatataa
     2221 aatattgcac cataaaaaat tagtattatt atagatgcta tagtattaat cgtttttatt
     2281 taccctattt cttctttttg caaattcttc aagtgcttca gatttaattt tatctatttc
     2341 ttcatattcc ataaaattag catcttcttc aaatccatct tgttcttcac cattaaaacg
     2401 atacattttt tttagtaaat tccataatgt ctccaattca tctttggtaa attcgtgaaa
     2461 tacatctgcc atgaagttta ttccagtcct attacatgtt accataacct tttttcctaa
     2521 gtctgtaact ctaacattaa ttgctctctt atcatgagga cttggaacta catcaacata
     2581 tccatttttt tcaagattgg caactagtct atttatattt tgtttacttg tacccatttt
     2641 tcttgcaata ttatttaatg ttgtttcttc ctctggtaaa tgaagaattg ataaaatagt
     2701 catatactgt cttgaagtaa gtataccaaa gtatttatct ccttctattt gtattttgtt
     2761 tgtaagggta aaaagagttg catatgtttg attcattaaa aagagctctt ttaattcatt
     2821 tgaataatcc atgaaatata tctcctttat ttttagatgt tgttttgtcg aaaacaggat
     2881 atcatgaatt aattctaatg tcaatatggt gtcaaaatat attaaagtat tactttttta
     2941 tgtaaatatt aattaattaa attattgtat aattttatgt aaaaatttct atgtacaatg
     3001 ataacttcta tgaggaatta ataattctta aattttttta gaaagatttt gacttgaaaa
     3061 tacttgtaat ataatatata aattaatata ctgaataaaa tcaatcggag ggataattat
     3121 gaagtgtaca aactttactt tcaaaggaga ggaaggttta gatatatata catataaatg
     3181 ggaagatgaa aatataaaaa aaacaaaggc agttatccaa attgcacatg gaatggctga
     3241 aactgcacaa aggtatgaga cttttgccaa ggtacttact gaaaatggtt atatagtata
     3301 tatcaatgat catagaggtc atggaaaaac tgcaaagata atagaaaatg ttggacattt
     3361 agctgaaaaa gaaggattta gatgtcttgt agaggacatg tatactttaa caaatattat
     3421 aaagaaagaa aatgaagatt tgccaattta tctatttgga catagcatgg gttcatttgc
     3481 aagtcaaaga tacataatgg attacggtaa taatttatct ggtcttatac tatgtggttc
     3541 gaatggaaaa caaggaatca ttttaaattt tgctcatatg ataatcaatc gtgagattaa
     3601 aaagtatgga agacgttcta gaagtaataa aataaatgat ttgatatttg gtggcgaaat
     3661 aataagaaga aatgaaaaga ctaagtttga ctggttaagt agagataaag aacaagttga
     3721 aaaatatata aatgacccat tttgtggagt agtgtgtagt tgtggctttt tttatgattt
     3781 agttcaaggg ttaaaagaaa ttgaggataa agaaaactta aaaaaagtgc cacttgatat
     3841 acctatttac ataatatctg gagataaaga ccccatagga aaaaatggta aaggagtctt
     3901 aagattaagg gatagatata taaaattagg agtaaaagat gtaacttgca aactttacaa
     3961 agatggaagg catgaattgt tgaatgaaat aaataaggaa gaagtttttg aggacataat
     4021 ttgttggcta aataataaaa tagaaatatt gagataaaag aatataatta tatattaata
     4081 ttaaatttga ttctatggaa atgtttgaaa aagtatattt aatatatgta aaagagaaaa
     4141 gaaagtgttt caaaatgatt atttattatc agtgaaacac ttttttattt tttgatgtgt
     4201 atatttacta ctttcaagag aagaatgtat taaaatctta agaaaatctt ttgatttaag
     4261 tacacatctt cttaagaaaa ttaaattatt ctagaaaagg aactagaaat aagtaggtgc
     4321 tattgatttt gataaatata agaagcttat ttatgaaatt gataagaaaa aaatacgtaa
     4381 aaaatataaa tttatttgtt tttaatttca aaataaaact taatggaggt aatggatatg
     4441 aacaaaatac tgagagtaaa aaacataaaa aaatactata aaagtagagg taacgttaca
     4501 aaagctattg ataatattag ttttgaagtt gaaaggggag agtatatagg tattatggga
     4561 gcttcaggaa gtggtaaaac tactttattg aattgtattt ctactataga taaaacgaca
     4621 gaaggtaata tatacatagg agataaagac ataacaacta tggatgaaga tgagctatct
     4681 aaatttcgta gagaagaact aggttttata tttcaagatt ttaatttatt agacacactt
     4741 acagcacatg ataatatagc attgtcttta tctattaatg gagttaattc aaaagaaata
     4801 gataataaaa taaaaaaaat agcaaataaa ttaggtgttc atgaaatact taataagtat
     4861 ccatatgaaa tatctggtgg tcaaaagcaa agaatagcat gtgcaagagc tataataaca
     4921 aatccatcac taatattagc agatgaacct acaggagctc tggactctaa gtcagcaaaa
     4981 ttatttttgg aaaaccttga agttttaaat catgaattaa aagctacaat attaatggtt
     5041 acacatgatg cttttacagc tagttattgc aaaaggatat tatttataaa agatggtaaa
     5101 atattcaatg agttggttag aggagataat actcgtcaag aattttttga tagaataata
     5161 aaagtaatat aagctcttgg gggtgatgat aaacatgttt tttagaaaaa ataaatcaag
     5221 aagagtaaga aataaaagaa aaaataatta tgtttcaaaa gtgtcaagtg tgtcaagtga
     5281 atcatttaaa aaattggcct taaataatgt gaaaaaaagt ctgaaaaatt atttgattta
     5341 ttttataacc cttgtatttg gagttattat tttatacact tttaattctt tagacaatga
     5401 tatagcaatt cttagtaata atgctttatt gagttcaagt atatcatttg ttagaggtat
     5461 tgtaagtatt ttttcaattc tagtgtgtat tatatttgca tttttaatta tttatgcaaa
     5521 taactattta atgaaacaac gtaaaaaaga gtttggaata tacagtattc ttggaatgga
     5581 cagaagagac ataaataaat taatgtttag agaaatgatg ataataggta tattttcttt
     5641 aataataggt attgtatttg gagtatttat ctctaaggga cttagtatat ttgccttaaa
     5701 gatgttaggt atatcatcat caggattttc tttctcaata cttacaacta ttaaaacaat
     5761 agtatttttt ggcattgtat tgttattagt caataaattt aataaaaaaa caataaaaaa
     5821 atacaagtta atagacttat taaatgcaaa taaaaagaat gaagtttcag ttgcaaaagg
     5881 gaaaaaatct aatttgatat tattcttttt atccatagtg tcaattgttt tagcatatat
     5941 aataccattg aaaatgaaaa tatctacgtt tgtactatgc atatgtttaa ttttaatatt
     6001 agttggaatt tatttgtttt ttatgtcagt atctgatttt ataatgttaa aaataaagaa
     6061 gcatagattt atatactata aaaatttaaa tccatttaca gtaagtcaaa taagtagtca
     6121 aatcaaaaca atgagtatgt ctatcactat gatttgtatg ctattatttt cttctttgat
     6181 agtaatacct tttggtatat cctttggaac atatttaata gatgatttac aagaagcgac
     6241 accttatgat gtaacattat ctaaattatt tatgaatgga gataacattg tagaatacag
     6301 tacaaagaaa agtgatataa atagtaaaat tccttcactt aaagatgaac taatagcaaa
     6361 tgattttccc ctaatttcat tttctaaaag tacaagtgat ttaaggatat ataatttaaa
     6421 gaacataagt cagtacgatt ttataacaaa tgataaggat aagacaatta aactgaaaga
     6481 taacaaactt catattgtct caattagtga ttataattct actagaaagc aaagtggttt
     6541 aaaagaaata aatctaaagg ataatgaatt tgcaataaat tgtgcctcag aaaattatag
     6601 acaattttat gaaaagtaca ttaagagtca gccacagaat tatataacat taaatggagt
     6661 aaagcttaaa ttaaatcaga caagcttata taccaattct tacaatacta atacagttcc
     6721 tttagatttg ggaaccataa ttgttccaga tgctgtaata aaggatttaa atcctattat
     6781 gattacatta aatattgatt acaaagaaag aagtagtaat cttgaaaata aatttatgga
     6841 ttcatactat aaatttgaag agaaaatcga taattctgag gattatagta ttttatataa
     6901 atcagttata gatggagaaa aaagagcttt gaacacttcc ttttcttttg tagcaatata
     6961 tataggtatt atattattaa tatcagcagg tgcaatacta gcattgcaac aacttataga
     7021 atcaactgtt aataaagaaa gatttaaatt attaagtaaa cttggagtaa ataagaaaga
     7081 tatgaaaaaa gctattttta tacaaatatc aatattattt gcagtacctt taatattagc
     7141 tcttataaat gctttgttta tatcaaaagt cttatttgtt ataatgccat ttattgcaga
     7201 gacaggtgtt attacaaata tgttactaac gttaggtctt attatcattg tatatggaat
     7261 ttattttatt gcaagtttac ttgaaagctt aaatatagta aataatagta aagagataat
     7321 ttaagttttt aaaagactat ttagtgttaa tctaattaat taagctacac ttaagttagc
     7381 ttaattaata gattctatat ttcaagccaa tgagatacgg ttttagatta aaatataacc
     7441 aatggttaaa ttacagacat tttgttaact ttccctcgat ttactagtaa atattttttt
     7501 tatatactta aattacacca aatgtactag ggaggtatat atgcaaatta aaataggtaa
     7561 agtcatacag catcttagaa aagaacgaaa tttaactcaa gaacaattag caaagtttat
     7621 aggtgtgtca acaccagcag tatctaagtg ggagagtgga aattcttacc cagatataga
     7681 attattacct ttattagcag acttttttaa tgtgtctatt gataagcttt taaattacaa
     7741 gattgattta agtgaagaag aagtaatgaa aatttataaa gaattagaat caggttttgc
     7801 tagaattgaa attgatttat caacagaaga accaaaagaa gagtgtagac aagacttaga
     7861 atctgtaaaa aaactttcta atatgtatat agagaagtat cctaaaagct atttgttgaa
     7921 gctaaggata tgttcgctgt atcagatgta ttcatataaa tttggtaaga gtgaacttaa
     7981 taacagagta aaagaaacaa cgaatattct tgaagatata gtaagaaata cagatgatat
     8041 ccaaataaaa gaaacagctc ttattatatt atcaaacgca tattgtatgt tggaggatta
     8101 tgaaaaggca gagttgtact taaatatgat acataagtca ataggggata caagtgttaa
     8161 tttagctatg atatatttaa agcaaaatag acttgaagaa gcagaaatat tgcttcaaaa
     8221 taaattgttt agtaatgtat tcaatatgag catggattgt aagggaataa tcaatgtata
     8281 caaaaatcaa tacaaggaat taaagaaaaa attggaaaat agaaattcta ataaaaatgc
     8341 aattgagaga aaaatggagt atataaaaaa taagttgctg gggtatgcaa atctttcttt
     8401 agaaataaaa aaaatgctca gtgaagataa aggtgcattt tttagtatgt atatggatta
     8461 tatggaattg tctctaattt ttttattttt taacatgaaa gaagaagcaa aaaaagcctt
     8521 atatagtcta aaagaaatat tagaaaaata tcctatacat gaaagtttgg atgtaagtca
     8581 aatgagatt
//
LOCUS       DAEMAE010000160         8556 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEMAE010000160 DAEMAE010000000
VERSION     DAEMAE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.162"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(174..1295)
                     /locus_tag="KPH93_002778"
     CDS             complement(174..1295)
                     /locus_tag="KPH93_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG1689575.1"
                     /translation="MATNKDYIEYVHKVYNYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGTTCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LLSKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(1356..1841)
                     /locus_tag="KPH93_002779"
     CDS             complement(1356..1841)
                     /locus_tag="KPH93_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG1689576.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SKAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(1843..2511)
                     /locus_tag="KPH93_002780"
     CDS             complement(1843..2511)
                     /locus_tag="KPH93_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689577.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDATGPLKVLDF
                     NNVLGSFGTLGTVNDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            2902..4926
                     /locus_tag="KPH93_002781"
     CDS             2902..4926
                     /locus_tag="KPH93_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1689578.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRNRLEDIPLLLESFLKELPYKNIKLDKNLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNVSIFNDLSQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTHNGYLISNKGRKGSSITEKGIKIIENNII"
     gene            complement(5109..6296)
                     /locus_tag="KPH93_002782"
     CDS             complement(5109..6296)
                     /locus_tag="KPH93_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG1689579.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDEEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDANIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(6498..7769)
                     /gene="brnQ"
                     /locus_tag="KPH93_002783"
     CDS             complement(6498..7769)
                     /gene="brnQ"
                     /locus_tag="KPH93_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG1689580.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQLA"
     gene            complement(7910..8554)
                     /locus_tag="KPH93_002784"
     CDS             complement(7910..8554)
                     /locus_tag="KPH93_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG1689581.1"
                     /translation="MIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEVISD
                     LDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKNSKL
                     IGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVFEYT
                     NLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
BASE COUNT         3049 a         1456 c          988 g         3063 t
ORIGIN      
        1 aatagattta aaatataatt acttttccat catatttttt tcaatattcc aaaaccagtt
       61 tcattatatc aaaataagat tgctcatatt aattatattc taattaacat aagcaatctt
      121 tttctataac ttctataatt ataattttaa atgtagaatt attagtttgg atattatccc
      181 aatctctttt caaccaatct acaccatata tctacaccat attctaaagc tttcgtatca
      241 aatgttacat ttggattatg aaatcctggt gtagcatcag ctcctaatcc aatatatgta
      301 gttttacatt ttagtttttg tgtataataa tggaaatctt ctccaccaga atttttaaat
      361 atacctatag atgcatcagc tcccaataca tcttttatag tttcttcagc caagtttaca
      421 agctcttcat caaattctgc tgctggtact ccatctttgg aaagcagtac agcttctgca
      481 tttatagaac ttacagcagt ttttacagca tgtattgctt tttcttttaa ttcttctatt
      541 atatcattac tttctgccct tatatctaaa gccataaatg cttcatctgg tatattatta
      601 taagtttctc ccttaactga tatatttgtt acttttatag aatttggtat agctggattt
      661 actcttattg catttattgc attcactgct aaacaaacag catctactgc atttactcct
      721 aaatgtggtt tagaaccatg acaagtggta cctttaatcg taaattccat tatacaacta
      781 gcacaatggt acattgcagg tatcgccttt cccaacctac aatcttgtcc aggtcttaaa
      841 tgcatcccta ccatttcttc tacatcatct aacaaaccag aatcaattac tcttaatgct
      901 ccatataact tttcttcacc tggttgaaaa agtattttta acttacctct ttttactaaa
      961 tttctctctt ttattaactt tgcagctgtt aaaagcatag aagtatgacc atcatgacca
     1021 caagcatgta tcattttctt ttctccatct atgataaatt ccaatgcatc catatctgct
     1081 cttatagcaa atactggtcc ttctttttta gaatctatag ttactataat accagttcct
     1141 gcaactcctt tattaatatt ttcaaacccc attttttcaa gctcttttaa aacaaactct
     1201 gatgtcttat attcttcaaa tcctttttct ggtatagtgt gtagataatt atacacttta
     1261 tgtacatact caatataatc tttgttagtt gccataatat agctcctcct taaaatttaa
     1321 actttccatt tatatcaaat ttttattata attacctata ctacgaaata attcattata
     1381 agcattgcaa tacaaccatt taaagttgct atcacaaaca tatgtggata atatttagtt
     1441 tttaactcag ctgttcctaa aagtctcccc ataaattgta tctgcgctcc tgaaagcaat
     1501 aacattggta atagaattgc aacatgtgct ttacttattt gacctgctgt aaataaacct
     1561 gcgattactc cagatgctcc tcccattgat ataaatattg tcattatagc tgttgctgca
     1621 acacctggaa gtccaaagat actcattaca ggtgtaaata ttgtccctat tacatccaaa
     1681 agacctgtta aattgaacac agaaattata gcaaaaccga agatgacatt tggagccatt
     1741 gaatttgtac ttatttggaa tccatttcta gctcctccaa taaacgcatc tatcacattt
     1801 ccttttttaa cttttacttc tttttttgct acctcactca tgttaagctt cccccttgtc
     1861 aaatttattt aaatataatc taagtaagtt cgccccaaca aacttcataa ttaatattaa
     1921 agcaagtggt ataaatattg gtacatctcc tataaatgga aatagtgctg ctccagaagc
     1981 aaagaaattt gtaattgcag acgcacctga aaattgaaat gcacaaaata cagccttttg
     2041 tttttcatct atatatccat catctttaag ttctttggtc atagatgcag ctgcatctgt
     2101 agactgtaaa ctagctatca aagttagccc tgcaaatcca ggtagcccta ataatggctt
     2161 taatagtgga ctaagaagct tttgagctgc cttcattcca tctaagtggt ctataactct
     2221 aactactcca agtgcaaaca taactgatgg tattaatgtc aatgcatata gccatccatc
     2281 tctaggacca gtacctccat cacctctaaa gtttgcagct aatgtaccta caccatcatt
     2341 aactgttccc aatgttccaa aagaacctaa tacattgttg aaatccaata cttttaatgg
     2401 acctgtcgca tctttaaata ttcctgaaaa aaataatgct cccaatatta atgctacata
     2461 cccataccat tttacttcaa ctttttctgt tgttgattct aatttactca tataaattct
     2521 ccctcctgtt taaatgaatt ttattagtat acttgtttaa taagcaaaac ttatgccaat
     2581 accttaatga taaagcctct gtatttattt ttttattgta aatttattcc attaattaaa
     2641 tagcttataa ttacttactt ttcaagttta aaaatataat atttttttat taattattta
     2701 aaacagacat cacaatataa aaaagctatt ttccttatct tgcaaaaata attttaaaat
     2761 aggtttttat aggataaaat cctaaaaaaa cctattattt aatcaaatat ataaatttat
     2821 actgctattt gttatataat ataacttacc ttatacttta aatacttcaa aaataaatac
     2881 tattttgtta ggagaaataa aatggacaaa aagaagttaa tccttataac atatgataaa
     2941 ttgaatagtg accactacaa agaagaatta acaaactttt ttggagacga aataatcata
     3001 gaaacacaaa atatacttga tggaataaaa gaaaatttag agggagatgt agtgctttct
     3061 ctatcacctt taactagtaa ttttttaata aaacatttta aggaagatat tgaaattatt
     3121 catggaacga aagcactttc aaaacttgga tatgaaaaaa tgatgaaact acctccaggc
     3181 acaaaatcgt tacttatgac aaccaataaa acttcagcat ttgaaatggc tacttatcta
     3241 tataaaattg gtataaatca tatagatttt gtacctacat atccagattg tgatgaaata
     3301 tatgatttag atacagctat aacaccaggt cagattagat ttataccaaa atacataaaa
     3361 aatattgttg atttaggttg gagaaaaatt tcattagata catatatgtc attattagta
     3421 gtattaaaac taaaaaatga gaaatttata gaaaaactat acaaattgtc caaggagaca
     3481 ctaagtcatg atttcttaaa tacatcatta gataatatat ctaaattaaa aactatactt
     3541 tacatgacca ttgatgaaat aggtgatggc ctaatatttt tcaatacctt taacaaggtt
     3601 acttttgtaa ataagtcttt acttaatatg ctggaactag atgaaaaact tataaaatca
     3661 ccttctttaa tggaatatat gccaaagagt ttcctagata aaattacaaa gaatttaaat
     3721 attgataata tgattatata catagacgag atagataaaa aattcatact ttccaaaaaa
     3781 ccattttatc tttataaaaa tatagagggt tgcttaatta cattaaaaga tgtaaacaat
     3841 atagaaatat tagaacaaaa aataagaagt gattctgtta aaagaggcta tgtagctaaa
     3901 tataaattta ataatattat tggaaatagt tctattataa aagattgtat taaaaaggca
     3961 aaaaaaatgg ctcttacaga taatcctata ttaataacag gtgaaacagg aacaggtaaa
     4021 gaagccttta ctcaatcaat acacaatcat tcaaatagaa aaaataaacc ttttgtagca
     4081 ataaattgtg cttcattacc aagtgaatta cttgaaagtg aactatttgg atatgaagat
     4141 ggttctttta ctggagcaaa aaaaggtgga aaaaaaggat tattcgaatt agctcatact
     4201 ggcactatat ttttagatga gataggagat atgccacatg atttacaggt taaactttta
     4261 agagtcttac aagaaaaaga aattagaaaa attggtggta caagtatcat tccaattgat
     4321 gtgagaatat tagctgccac aaataaagat ttagaaaaat taattataga aaataaattt
     4381 aggatggatt tattttatag gataagtatg ttcactttag atttacctcc tcttagaaat
     4441 agattagaag atatacctct tcttttagaa tcatttttaa aagaacttcc ctataaaaac
     4501 ataaagcttg ataagaatct tttagaagct ttaaattcat atacttggat ggggaatatt
     4561 agagaattga gaaattgtat tgaatatatg gcttacatgg gttctaatta tcttacaata
     4621 aatgatttac ctcaaaacat atcttcaaaa ttaaataata atcatatgtc aagtaatgtg
     4681 tccatattca atgatttaag tcaatatgac aaaaacatat gcatttctat cctaaaatca
     4741 ttgcacatga aaccaatggg aaggacaaaa ttaatgaaat ttatggaata taatgtcaca
     4801 gaatatgaag taagaaatat gctagaatat ttgacacaca atggatactt aatttctaat
     4861 aaaggaagaa aaggctcatc aataactgag aaaggcataa aaataataga aaataatata
     4921 atttaaacct atagtaattc atctttactt tatcaaaatt tatatataat tataattttt
     4981 tatttttcta taaatttttt ataaaataat aaaacttatt tatattaaaa agataaaaat
     5041 atatttgatt tatcacaaaa agagtgcctc ataaactaaa aatttcattt tgaggcactt
     5101 ccctaatatt aataaattaa ttggcttaaa attactgatt gataattata aagtacctct
     5161 gttaaaggag taaattctga accgattcca aatcttaatt ctgtaaacgc ctctaaatag
     5221 tctgggtcac atacatcttc ttgtgttaaa tcttgtttta ccatttcagt agcaagataa
     5281 attgctaatg atggtagaaa agcatccatt ggcataggat atccgccaag tctatttgac
     5341 ataccaaaag ccttagcctt ttcacttatc atgtcattta ttttatcata attttgagca
     5401 tctttttcag atatttttaa acccataaca ctaggatacg tttgtattgg tgatggattg
     5461 ctttgctctg caactatata ctttaattct aaagccttag tcaatataac ttcatccata
     5521 tactcattta caccaaatac atttatatct tttccatatt ttttaacttg tttttcaata
     5581 tcttcattta aaaattgctt aactttgttt ttgtcttctt cagtatttat atttggagta
     5641 ttaacttgta caaatggcaa tcctatattt ttgcaagtct ctttaatcat ttctaatctt
     5701 ttggctatat tagcatcttt caaatcatct gtagaggcat aatgaataaa agcttttgct
     5761 cccatttctt ttgaacgttc agctaatact tttcctcttt cctctgcact aacccctaga
     5821 ttcacatcaa caaattggct taattggttt ttatcatctc ccataggagc agaaatagtt
     5881 attatttctg gacgcttttc ctttaccttt tgtaaggcag gtaaaaggcc tgcttggtca
     5941 gttgaaacta caatagcctg aacttcttca tcatcagcca actttactat cttattaata
     6001 gcactatcta tattagaagt aaaattctct ggcaatacaa catgctttat agtttgttta
     6061 actttagttt gaccatcttt gtctttatca attttattct cttcctcata ttcttttgcc
     6121 atctcttcca ccatattgta ttgtacttta ttttcagata atggttgagt taccactgca
     6181 actttaaaat catctataat tttctcttta tctttcttgc ctacaagtaa actacaacct
     6241 gtaagtccaa taattaaaca tatggatagc ataaatgctg ttatttttct aagcattaat
     6301 taaaacctcc taaatttgta ctttcttatc actattacta attttatcat acttcaaaga
     6361 aaaattatat tataaaaaat tatatacatg ctattctaat ccaatctaaa aagtactatg
     6421 ggtagattag gtttagatta aacctaccca tagtactaaa aatcaatata ttataaaatt
     6481 aaattaatta caaattatta tgctaattgt ttgttgcttt ttataaagtt accaactatt
     6541 ccacccaata tagctggtat tacccagtta aagcccaaac ctgcaagtgg caagctttgt
     6601 ataaatggaa cagctcctgt cattccattt atagctgata ataaactaat cactaatgta
     6661 acatatgctg cacctttaaa agcattatca ttctttatat gtttaccagc caactccata
     6721 acaactattg ctaataaaac aggatatata gtttgtagga ttggaactga tatagctatt
     6781 atagaatcaa cacctaagtt tgatataagc atactagcta cacatatacc tataaccagt
     6841 tgtttataac tcgccttact tattccttca aaataagttg atattgatgc tgttaaacct
     6901 gtagaagtag tcagacaggc aagtgctaca attacaccta aaattacctt tcctacttga
     6961 ccatataaca atatactagt tatttcaact aataaagatg cttgaggtat actagtatca
     7021 tataaagttg aaactgttgc acctagataa gtaagaccac catatactac tgctagaaat
     7081 acacaagcta caaaaccagc tttaatagtc attcctattt ggtctttatc tgatgtatat
     7141 cctttagcta ttatagacat caacattata gttgccatag atgctgcacc tagagcgtcc
     7201 attgtttgat aaccttgtga tataccttca ccaaatagat ttttatctat catatgctca
     7261 ggactcatag gtcctgctgg agaaattata ccctttacta ttaaaacaaa taatactaat
     7321 aataacgctg gagttaaata cttacctatt atgtctataa ccttagaaga cctaatagta
     7381 aatgctaaag ttaacccaaa gaagattatt gaaaatatag ctttagctac tgggccatgg
     7441 tctccaaata taggcataat acccatttca tatgttgttg cgcaagttct tggtattaca
     7501 agaagtgcta aacatgctag agcagctaca ctcattactc ttgctaaccc tttaccagct
     7561 ctcctaagta catcatccat gcttcccttg catcttgctg ttgctataat aacaagcatt
     7621 gctataccag catctgcaac aacaaatcca ataagaggaa ttaaccagtc tttgccggat
     7681 tccatcccta aaaatggagg aaacataaga tttcctgcac caaagaacat agaaaataaa
     7741 gcaaaaccaa ctataaaaat atctctcatc tgcttactca cactaaatta ccccctttga
     7801 aaatattttg aatattaaga taatcttact ttattttcag aaaaatgtca atctggtaaa
     7861 aattcacaca ttgttaacgt tttcccaatt tatttcatga atattttttt cattttatga
     7921 taattcccat caatttcgtt aaatcaattg cttgcgacac atcaaaaatc gctcctctaa
     7981 tatcatctat accaatctca attccctcaa tattattagt agtaaaatct atgccagaaa
     8041 gtttagttcc attaaagttt gcttttttta agtttgtata ctcaaatact agattgtcac
     8101 cttttacttc ttgaaataca gaattttcaa aatttgaatc taagatagaa actatcttaa
     8161 atttagagaa cgaaaagtta gaatacttac ctaaaacatc cttgaacaaa acgtttttaa
     8221 gtatacaatc atcaaatcta actcctatca atttactatt tttaaattca actctgtata
     8281 tacttcctcc atcaaaaaat gtatttgata aattgcagtt ttcaaatacc acatcaagta
     8341 aatcaatctt ttttaaattg caattttcaa aggatacatt tctaaatata caagattcaa
     8401 agctaactct tacagaatct aaatcactta taacttcatt ttctataatt ttattaaata
     8461 ttttttcatc attaaatata tcttcaagta tatcttcaag attttcaata atttctaatt
     8521 ctcctgtaaa tttaggtttt tgcactttaa taattt
//
LOCUS       DAEMAE010000161         8415 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEMAE010000161 DAEMAE010000000
VERSION     DAEMAE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.163"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..958
                     /locus_tag="KPH93_002785"
     CDS             92..958
                     /locus_tag="KPH93_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG1689582.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCSNLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIIDLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKTNLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            1071..1475
                     /locus_tag="KPH93_002786"
     CDS             1071..1475
                     /locus_tag="KPH93_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG1689583.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            1651..2100
                     /locus_tag="KPH93_002787"
     CDS             1651..2100
                     /locus_tag="KPH93_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689584.1"
                     /translation="MKSEIILEMKQINSTEYDEMLQVWESSVRATHDFLTKKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKAFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2257..2634)
                     /locus_tag="KPH93_002788"
     CDS             complement(2257..2634)
                     /locus_tag="KPH93_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1689585.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            2896..3813
                     /locus_tag="KPH93_002789"
     CDS             2896..3813
                     /locus_tag="KPH93_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG1689586.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDSPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVAKQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWNTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSSYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGDEMVGPWVSRIDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            4297..5715
                     /locus_tag="KPH93_002790"
     CDS             4297..5715
                     /locus_tag="KPH93_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG1689587.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GIFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIVPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            5791..6603
                     /locus_tag="KPH93_002791"
     CDS             5791..6603
                     /locus_tag="KPH93_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1689588.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDEVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVIGNPRESYIDGIWNKENEEDWLTEVQIPVKRIL"
     gene            7035..7652
                     /locus_tag="KPH93_002792"
     CDS             7035..7652
                     /locus_tag="KPH93_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG1689589.1"
                     /translation="MEIFAIGHSNYPYDKLIDMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFVYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(7809..8261)
                     /locus_tag="KPH93_002793"
     CDS             complement(7809..8261)
                     /locus_tag="KPH93_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG1689590.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
BASE COUNT         3316 a          876 c         1403 g         2820 t
ORIGIN      
        1 ttaagaataa gaactgttaa ataagggact tgattatcat ttagttatag ataatataat
       61 attaaaaata aagcttaaga ggagaaaaaa tatgatagat atacataaaa atatatatga
      121 taataagtta tttgaggagc taaagattga ctgtaaaaag tgttttggac tttgctgtgt
      181 agctctttat ttttctgctt cagatggatt tcctattgat aaggaatcag gaaaaccctg
      241 tagtaattta caaccagatt ttaaatgttc tgtacataat agtcttatga agcgaggttt
      301 taaagggtgt actgcatatg attgttttgg ttcaggtcaa aaagtagcac aagtaacata
      361 taaggggata gactggatgc aatcaccaga attaacaaat caaatgtctg aagtgttttt
      421 aattatgaga caattacatg aaatgttatg gtatcttaaa gaagcatctg tattaaatat
      481 atcagataca atcaaatcaa aaattgattt aataattgaa gaaactgaaa agataactaa
      541 tatgagtcca gaacagataa ttgatttgga tatcatatca catagaacaa aggttaattt
      601 attattatca caagcgagtg agtctgtgat gggaaaggtt aaatcattta tcaagacttc
      661 tactcttaaa aacatgaaaa aattaagtaa aaatatagat ttaataggag cagaccttag
      721 aaaaacaaat ctaataggag cagatttaag aggtagattt ctaatagcag caaatttaag
      781 aaatacagat ttgagtgggg caaatttaat aggagcagac ttaagagatt gtgatattag
      841 aggtgctaat cttgaaaata gtatattttt aacacaatta caagtaaata ctgcaaaagg
      901 agattctagt acgaagttgc cagcttcact aatacgccct aaatactggg agaaataatt
      961 aaattaatat ataattttac aagatatgaa cttttttgac tttaattcat attatacatt
     1021 agagattctt tacaagaatt tcttaatata atatagggag agatataaaa atgagttatc
     1081 aaaattataa atgtgaaaga gattatgtgt atgatgatta taaatacaat aagtgcaaca
     1141 agtgctataa tgattatgag gaaatgacac atgttcatga atattcagaa agtgttaagt
     1201 tagcagaaga atgtgaggat agacataacc accgtgcagc aggagttact ggtgaagtta
     1261 taccaataaa tggaggtaca aatcatgttc ataaaataaa tgataatgta gatttccttg
     1321 accatttcca taagatatgt gttacaacag ggccagcaat tagaatacct ggaactgata
     1381 aacacattca cttaatatgt ggagaaacta ctgttaatga tggtcattgt cataaattcc
     1441 tttttactac tcaaatagaa gctcctttag tataagctga tgtgaattat tgtagtaaat
     1501 ttgctagatt atagtatata gatgttaaaa atatataata gataatattt aaacttaatg
     1561 aggactatga tatttaacct tcgtagtcct ttctaagttt tgtatgaaga agtgttatac
     1621 aattgaaagt gatataggga gaaaattttt atgaaatctg aaataattct tgaaatgaaa
     1681 caaataaatt caacagaata tgacgaaatg ttgcaagtct gggaatcgtc agtaagagca
     1741 actcatgatt ttttaacaaa aaaagatatt gaatctttga taccattagt tgaaattgga
     1801 cttaaagaag tagaaaatat agtttgtata aaagataatg atataataaa gggctttata
     1861 ggaatagata aagataaaat tgagatgctc tttatagaag ataaatatag aggaaatggt
     1921 attggtaaaa aattaataaa atatgctata gataaataca atgtaaaata tgttgatgta
     1981 aatgagcaga ataaaaaagc tgtaggattt tatattcact tagggtttaa agcgttcgat
     2041 aggtctgaaa tagacggaca aggtaaccca tttccaatac tacatatgaa attaatttaa
     2101 agttatactt ctcacttatt cgcaaaaata tttacttaaa ttttttatac aaataaaagt
     2161 cgtgacagtc ttaggacacg actttcttac tacactatta aattgaaatt tatctgttct
     2221 atattatgat tattagatgt agttttttta ttttaactat gtacttaaat accaagtata
     2281 cccataccta tcaagtactt cagcagcgcc atcattttga tgtggtggtg ggtgtggtaa
     2341 aaattcttgt acaagcgtac caccctcggc aagttttaca gatatttctt ctattgcttc
     2401 tcgagatggt agaacaacac aaatcgccat gccagaaatt acaacaggct tgtctgtatc
     2461 cgcacccatt atacaacctt catattttgt gaaagtaaca cttgcgtgca taacaggatt
     2521 gtttggattg ctgtctaatc gcgcaaatat agtttttcct cctccgaaag cctcagcata
     2581 aaaattaaat gctttttcac aagtaccatt aaatattaaa tatggagtta gcatgacaaa
     2641 tatatcctcc ttgatataga ataatttata gttgcacctg atagtctaaa ttctgattta
     2701 gtaaattgta tcataggtaa accttgatgt atataatttt ttattattag attatacaaa
     2761 tttattaatt aaaaaaaatg ttatatgtat ggaagattta caggataaaa tataataaaa
     2821 agattaagat gacattataa aaaatgtata ttgacattga cgctacgtaa ttgtatatac
     2881 tttgtcttaa aggagatggt aaatatgaat attatagaaa ttcgctcaga taaaatatac
     2941 aagaagataa tggattcacc aataaacaaa aaagaagata tatacagata tgaattgatg
     3001 aagccttttg aatttaagtg gaagtgtatg aatgttccaa tagttgctaa acagaaaggc
     3061 ggatatgatg taattatagc aagtgaaatg ttaggggttt tatcgcctaa ggatattgat
     3121 gaaaagcaaa aaaagaatat aaatgtgtta tctgctgata aaatttggaa cacttgcaaa
     3181 gaaactatag aaaactctat aaatgctttt ataaaagaag ggtatgattt aaacattaag
     3241 gattataaat attcaatatt attggcgaat ccaaatagtt cttacacaat attaagtgat
     3301 ggatactggg gtgatggtgg gattcctgga tatatatttc tatcattggt tcctaatgaa
     3361 tatactatca atagattacc agtattaata gcacatgaat gtaatcacaa tattagattc
     3421 cagtttatag agtggaataa taatataaca ttagaagaaa tgatgataaa tgaaggtctt
     3481 gcagaaaatt ttgcaacatg gatgtttgga gatgaaatgg taggaccttg ggtcagtaga
     3541 atagatatcg aaacactaaa tacgtatata aagccaataa taaaaagtgc tttaaaagaa
     3601 actggatttc aaaatataac atcttatctt tatggtgatg atatagctaa aatgcaagga
     3661 tattttccag tagggttgcc ttattgtgca ggatatgctt gtggatatta tatgattaag
     3721 tattatttag aaaagacaaa taaatcaata atcgaagcca ctttattgcc ttatagtgag
     3781 ataatagaag cagtaaaaga gttttgggaa taaattttat aaattgcata atttgaaata
     3841 aattatgcaa ttattttatt tttaagaaaa aataaattga caataattta taagaaatat
     3901 acaataatat atataaagaa atatttcaat atttaaaagt tttaagattt catatctaga
     3961 gtacctaaca ttacatgtta gtttcttgtt acttatctaa ttactttata tttcgtaatt
     4021 atatataaaa ttactataaa aaatgaaata tattaaacaa tcaactgaaa aatgaaatgt
     4081 taataaaaaa aaattcaaat tataaatatt aaatgaatat aattaaaaca aatatataaa
     4141 tttaagtata acactcacat tttaatgtat tgtgaaattt ttgttgagct ttatgtacca
     4201 taacttataa aatttatgtc atgttatatt tgaataagta ataaaattgt agatgtaaat
     4261 taatctacaa aatttatatg aaatggagtt gatgctatga cagaattaat gattataagt
     4321 gggttagtac tattgatttg tataacttct agcaaggtat tgtataaatt tggagtacca
     4381 atattattga tttttatctt actagggatg ttatttgggt ctgatggaat ggtaggaatc
     4441 tattttgata actatgaatt aacaaaacaa ttatgttcta ttggactaat atttataatg
     4501 ttttttggag ggtttggaac aaactggaat atggctaaac ctgttgctat accttctata
     4561 ctaatgtctt cactgggtgt aataattacg gctggtgtca caggtatatt ttgccacatg
     4621 gtaataggaa ctagtttact tgaaggttta ttaattgggg caattgcagc ttcaacagat
     4681 gctgcatctg tgtttgcggt gttgcgttct caaaaactaa acttaaaggg ttcacttgcc
     4741 tcattattag aagttgaaag tggaagtaat gacccaatag cgtatatgtt gacactaatt
     4801 cttttgacat tgatgaataa ttctggggta gaacttattg taccaatggt aataaaacaa
     4861 atagcttttg ggttagctat aggatttata ctagcaaaat tatctgttta tattttaagg
     4921 cattcaaact tcgagataga aggattttat actatattta taacagctat tgcaatactt
     4981 tcttatgcat ttagcgagta tcttggagga aatggatatt taagtgttta catttcaggt
     5041 attattatag gaaatagtag aatacctctt aaaaaaagtt tggttcattt ctttgatgga
     5101 atttcatgga ttatgcaaat aatgttattc tttatgttag gattgttatc tttcccttct
     5161 aagttaccaa atgtatttgg tatagcaatg gccatatcta tattcatgat agttattgca
     5221 agaccattag caacatttat agttctagca aaatttaaat tctcaaataa agaaaaatta
     5281 ttcatatcgt gggtaggcct tagaggtgcg gcttcagtag tttttgcaat ttatgctgtt
     5341 actcaaggag tagccataga gaatgacata ttccatataa tattttttat agcgttgctt
     5401 tcagtaggaa tacaaggttc actgatacca actgtagcca gaaagctgga tttagttgat
     5461 gataatacac ctgtattaaa aacatttaat gattatgatg gagaaattag ttctaaactt
     5521 atcgaagtaa atgttgatga gggtaataaa tgggctaata agagtataat agattctaat
     5581 ataccagacg atatattaat agttatgata aaaagaaagg aacaggtttt aatacctaaa
     5641 ggttcaacca ttataaaaac aggagatatt ttggtattga gtggagatag aattgaagaa
     5701 ttaatacaat tataatttat tttgtattga ctttaaccta aggggaaagt ttaaacttaa
     5761 attaatcaaa tctaatgcgc aggtgatgag atgttttcta taggaatgtt ttctaaaata
     5821 aataaaataa ctacaaagac attaaggtat tatgaagata tagggttgtt aaaaccagaa
     5881 tatgtagatg aatttacaaa atataggtat tatacaacag agcaattacc taaattacat
     5941 cagataataa ctttaaaaca aatgggttta tcattagctg agataaaaaa agctgtagaa
     6001 aatcctgaag aagttgaaaa tattttaatg aataaagaaa aagaaatgtt ggatgtaatt
     6061 aaaagtgaag aatataaaat aattaaacta cgtaattacc ttatgaattt gaaaggagaa
     6121 gtcaatatga atgatgtaat tattaagtct ttaccaaaag ttaaagttgc ttctatgaga
     6181 aaagttttaa agggatatga tgaacttttc aatctatttc caaatgtaat ggctgaagaa
     6241 atgatgaaaa cagggtgtac atgtgcagtt ccagattatt gttttaatat atatcatgat
     6301 ggtgaatata gagagactga tatggatgtt gaaatgtgtc aagctataaa tgatttaaag
     6361 gaagatacgg atatattaaa atttaaagaa ttagatgaag ttgaaaatgc agtttgtata
     6421 ttacacaatg gtccatattc aacattagga aaaacttacg cagatgcatt taaatggata
     6481 gaggacaata aatataaagt gattggaaat cctagagaat cttatataga tggtatatgg
     6541 aataaagaga atgaagaaga ttggttgaca gaagttcaga taccagtgaa acgcatatta
     6601 taatctgtct acaaagtata aataagaata aaaaagaata aaaaaggtgg tgtctcaaag
     6661 taaacttttt agtttatgag acactctttt tcatatgaaa tcaagtatat ttttataatt
     6721 ccaattatga aaaaaggctt atctctattt tgggagagcc tcttattttt ttattataat
     6781 agttttctaa agatgctcta tctataaatt tttatcaaag attttataaa taattgtatt
     6841 taatatacaa aatatatata aatattgatg ttttatatat aaagttgtat atttttttaa
     6901 ggatataaag taaaaaaata taaagtataa agaaaaaaga tattggatat ttaaagtaat
     6961 actttattta attttaagta aatcgggtat ctacatatat agatactaat ttcaaagaat
     7021 gggagggtat ttatatggaa atattcgcaa taggacattc aaattatcca tatgataagc
     7081 ttattgatat gataaaaaaa tatgacataa attgtgtagt tgacattaga gaaactccat
     7141 attctaaata taatattcaa tacaataaag aggcatttaa cgaatctctt agaaattccg
     7201 gatttgtata tatatacatg ggaaaagaat ttggagctaa aagaactaat aaagatgtat
     7261 atacgcaaga aggttatgca gattttgaga aagtagcaaa agaagatatt tttttaaacg
     7321 gaatagaaag attaaaaaag ggttgtcaaa tgggttatag gatagtttta ttaggagcta
     7381 tgcaagaacc tataagatgc catagaagta ttttggtggg aaaagtatta aataaagaag
     7441 gttttgatgt aaaatatata atgcatgaag gaaatttggc atatcaggaa gatatagagg
     7501 aatcgttatt ggataagtat tttagtgata gaaagcaact gagtattgac aatttattag
     7561 ggagtgcatt aacgagagaa gaaatgatac aagaaggtta taagctagct aataaagaaa
     7621 taggatatag aactgagaaa ctaggtaaat aaaaatataa aagtagataa aatacagtac
     7681 ttaccttaag taaaataata ttatgaattt ataatagata ggcatgatgt gccaattaaa
     7741 aatattctag ttatttaata atatcattaa atttagggta ttttttaata gcacatcatg
     7801 ttgcagtttt aaaaaataaa atctctaata ttgccataat caactgttct attaagagta
     7861 ggatgactag atataaatct aactgtaatt tttgaattta acaaacaagg atttgaaatt
     7921 aaaaatactc tatctccatt gacaatagaa gtaatggcta ctggcaaaat atctacaaat
     7981 gtatcatatc tgtttttagc aatttgataa ggataatttc ctacaaaaac aaatagattc
     8041 aaatcatata tattatcagt aaaccaagcc cactggccat aaactttgtc gatattcatt
     8101 gtgcctaaac caagtgtatc aaagtatgtt gctatagtta aataaatatt ttccatgttt
     8161 tctaaatatg aaacagtata tcttctgaaa cgtatgggtg ctgtggaaga aataggataa
     8221 accatattta catttagatt atttgcattt aaattaacca aagtatcaca tcctaaaatt
     8281 agtctataaa atacaatatt taaatggatg cgattttgtt acatctaaag tgtaatattt
     8341 tatatattga aaaagtggta aaagtttgaa tgattaaata atactataaa aaatttataa
     8401 aatgtactta ttgtt
//
LOCUS       DAEMAE010000162         8291 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEMAE010000162 DAEMAE010000000
VERSION     DAEMAE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.164"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1282
                     /locus_tag="KPH93_002794"
     CDS             92..1282
                     /locus_tag="KPH93_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1689591.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTNIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGRMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1452..2414
                     /locus_tag="KPH93_002795"
     CDS             1452..2414
                     /locus_tag="KPH93_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG1689592.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2535..3197)
                     /gene="zmp1"
                     /locus_tag="KPH93_002796"
     CDS             complement(2535..3197)
                     /gene="zmp1"
                     /locus_tag="KPH93_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG1689593.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALNQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3297..3382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3568..6486)
                     /locus_tag="KPH93_002797"
     CDS             complement(3568..6486)
                     /locus_tag="KPH93_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG1689594.1"
                     /translation="MKKENRKTLLISLIMILSMLVSTIYPTVSYASELGENSQIQSSS
                     TNSSTGEDKESDNKKPEQIPEKDKATDNKKPEQTPEEDKSTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     ETNAKIHIETTGKIESGSGDVDVEIKPGDKEDIPDAKGTLKKLVEGRKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDITLYDELPEGLTLINGSVSVVTSDGKELSDFNIEQNENSI
                     SVDFGNIDKSYTVKYKARISDKNVKHDSKYKNVASIKSDGKKIQEDDATVSVFDRGDN
                     YLLTKGHSGATNITQVGQVINYQISVNDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEVPMNGSWTPWSKEKIANNISYKIEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSKIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNQALKISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGARFNLEDAFGNIVVKDLVSDEDGVIQSSVKKTGIYYLVEVMAPSGY
                     EKLKEKVKVEIGNKDIGKIVDIGNIENIRQENPPVNPPVPPNTDEPIVNPPVPPSTDK
                     PRKPSSPSDTDETAVINPPVPPSEDMINPPIPEIVNPPIPPSEEMIETPVKQIVPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIVAIVGLIILRRNKNTN
                     "
     regulatory      complement(6604..6689)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6817..7995)
                     /locus_tag="KPH93_002798"
     CDS             complement(6817..7995)
                     /locus_tag="KPH93_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1689595.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         2601 a         1315 c         1015 g         3360 t
ORIGIN      
        1 aacaaataat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata tatacataag
       61 caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc aaactttagt
      121 tgcaaacagt tctgttataa gagctatgtt tgaagaagga aagaaacttt ctgatattta
      181 tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac ctccagagac
      241 tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag ttcatggata
      301 tatgaataac tcaggatatg aggatgtaag agatgctatt gctgaacaca taaataaaaa
      361 ggatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg ctggtggtct
      421 aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt ttgcaccgta
      481 ttttggcgag tataaaaatt atacagaaaa ctatgatgga aaattaatag aagttcctac
      541 aaatatagag acttttgaac cagatttaga tgctctaaaa aatgctatta ctccaaaaac
      601 aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt ctgaagaatt
      661 attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata caaatatata
      721 cctaatttct gacgagccat atagagaaat aatttatgat ggtgccaaag ttccttgtgt
      781 actaaaatac tatagaaatt catttatagg atactcttat agtaaatcat tatctctacc
      841 tggtgaacgt ataggatata ttgttgctaa tgggcgaatg gatgattttg atgatgttat
      901 gtcttcatta aatgttgcaa ataggatact tggatttgta aatgctcctt ctctttttca
      961 aagagttatt gcaagaagtt tagatgctga agtcgatgta aatatttata aaaagaattt
     1021 tgaccttcta tataatagtt taatcgatat gggatattct tgtgttaaac ctaatggtac
     1081 attctatttg ttcccaaaag ctccaattga agatgataaa aaattctgca atgatgcaaa
     1141 acaatttaat ttattattag taccaggttc ttcttttggt tgtccaggtc attttagagt
     1201 atcatattgt gtatcttatg ataaagttaa aagctctttg ccagcatttg aaaaattagc
     1261 caagttatat aatttaaaat aactaaatca aagtaattca aataattaac taccttagta
     1321 tagaatactt acattctata ttaaggtagt ttataatttt atgtactata ttttatacat
     1381 accatattat tccaatatgc ttgaatattc ttatctatag tactaatata aattacaagg
     1441 aggtgtttga cttgaaaaaa ggttatgttt atataatact gactacctta ttttttagtt
     1501 caatggaaat tagtctaaaa actgtcacta atgactttaa tcccttacaa ataactttat
     1561 caagattttt tgttggcggt ttagtcttac taccttttgc tgtaaaaaga ttaagagctc
     1621 ttagtctttc tattacaaaa tcagacttaa aatactttgc atttttaggt tttatgtgtg
     1681 tagtagtaag tatgtcattg tatcaactgg ctgttctaaa cacaaaggca tctgtagttg
     1741 ctgttttgtt cagctgtaat ccggtatttg taatgctttt agcttactta attttgaagg
     1801 aaaaaatata taggcacaat gtaatttcac ttatattaga agttcttgga attatagtta
     1861 taataaatcc attgcataca aaactaactc ttagtggaat attcttaaca ttatcttctg
     1921 ctattatttt ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa tttggtggaa
     1981 tagttgtaac ttgctttagc tttatatttg gtagtttgga aatgttgata ttggttttac
     2041 tcactaaaat taattttatc gcagatatat taaataaaaa taaacttcat gtatttgcaa
     2101 atatccctat ttttagtgga tataccttac acaatatgcc cattatgatt tatgtattcg
     2161 tatttgttac aggtgttggt tatgcacttt actttatggc aatggaagct acatctacta
     2221 gtcttacatc tttagtattc ttctttaaac cagtaatatc tccaatacta gcattattaa
     2281 tacttaaaga aattatccct ataaacatgg ttataggaat tttattgata gtgattggtt
     2341 caataatttc tattatacct acaataatta ctcaaatgca taataaacac gttgaagcct
     2401 tgtataagga gtagtaattg ataatctatc aagttataca taagagaaaa tttattagat
     2461 ttaaacgcaa tataaaagag atgctattat aatagcatct cttaatttat atttatttta
     2521 aatttattaa agctctattt agctaggttt tgcaaaaagc tataagtttg tgggcaagct
     2581 gattttaact tactatttgt atcttgattt aaatagtaat atgcaaatgc ttctgcaaaa
     2641 aattcttctg gatatacacc taagtagttt acattaccta aagaacggcc ttctttagca
     2701 aatatttgtt tgaattgtgc tgatttagat atatcattta atactatatg gtctattgca
     2761 tgtgctgttt catgtaattc taaatttatt gcatcatgcc ctttaccttt gttactaaat
     2821 cctattctaa gagctactgt acttccacct aaacctggaa catcatccca agtttttcca
     2881 gtaccttccc atcccttagg aactacacct tttaaataag catattcttt ttcatctgtt
     2941 aactttccac ttaataattt tatttttaag ttattttgat ttaatgcatt taaatacttt
     3001 ccatctatat tagctaatct atttaccata gcattagctt cgtttttatc ataatttcca
     3061 gttggaaaaa ctactatttg actaagtgtg tctttatttt gttgtatagt agtactatct
     3121 gcatgtgcac ttactggaac tgaacttatt aagaatattg atattatagc tattaataat
     3181 tttttacttg gtctcatatt attccctcct ttgagttagg ccgggagtaa taaatttcaa
     3241 aataaaaaag atacccaaga gtatctttgt atatacaaaa agaaattttt actcttttcg
     3301 gcaactggct ggcatggttt actaaacttt agcccctata gctttgcgtg actaggtttc
     3361 cctagttttg cctcatttaa ttttatatct atattatagc ttatatcttc ttaattgtct
     3421 atatcccttt ggaacttttt tctaaaaagt tattattttt ttacataaat cttcaaaaaa
     3481 aaatatcttc taacaatatt ttcattatta aatattattt tagaagatat tttttaatat
     3541 aacttacatt ttttataatg ttagggtcta atttgtattt ttatttcttc ttaatatgat
     3601 aagtcctaca atagctacta taactaaaag tatttctcca ataattgtag tactatcccc
     3661 tgtttttggt ggtactggtg gatttaccaa agtatcatct ttaactttat tatttttttc
     3721 ttctgacact gatggcttta ccacttctgg tattggaaca atttgtttaa caggagtttc
     3781 tatcatctct tctgatggtg gtattggtgg attcactatc tctggtattg gcggatttat
     3841 catatcttct gatggtggta ctggtggatt tataactgca gtttcatccg tatcagatgg
     3901 acttgatggt ttccttggtt tatctgtgct tggtggcact ggtggattta ctattggttc
     3961 atctgtattt ggcggtacag gtggatttac tggtggattt tcttgtctta tgttttctat
     4021 atttccaata tctactatct taccaatatc tttattacca atttcaactt taactttttc
     4081 cttcagtttt tcatatccac ttggagccat tacttctact aaataatata ttcctgtttt
     4141 ttttacacta gattgaatta caccatcttc atcactaact aaatccttaa ctactatatt
     4201 tccaaatgca tcttctaaat tgaacctagc acctgaaagt acatttttat ctaaagcatc
     4261 cactttttta gcttcaaaag cataaccttg cttagtagtc tctactcctg gaggacttga
     4321 accattaatc tgagccacat tttgtatagt ctctccaact tttacatttg tgaagtctgc
     4381 atcaaacgta acccaacttt gcacaggttc accttctttt atctgtttaa attcgtattt
     4441 atttactaat cttattgtat tttttccatc tccagcttct tttttaagtt caagattatc
     4501 agaatatttt atattagata tttttaaagc ttggttcact tcatccaaaa gatttacttg
     4561 tcctgcatca taaaccccat aagttgacat attgattttg tatttaacaa tctgattatc
     4621 actactattt aaaacctttt tatcaacttc tttttttgcg cttattaatc cagaattttt
     4681 cttaaaattt atctcatcat ttttatcaat tttactatta ttggcatcaa gaacagctct
     4741 atttatatag ctatcttcaa tactaattac ctttaatgta taagctataa aaaactttga
     4801 ttccacttct tctttactga gattaattgt aaatccagtt ataactttat ctacttgccc
     4861 actttcattt cttttttctt ctattttata tgaaatatta ttggcaattt tttctttact
     4921 ccaaggagtc cagctaccat tcattggaac ttctacaact ctaaaatcat gccctgcttc
     4981 ccctgaggta gtcaatctca taccttctgg tatattatct gttataacaa cattacttat
     5041 tggtgactta tcatcattta ctgaaatctg atagtttatt acttgaccaa cttgagtaat
     5101 atttgttgct cctgaatgtc ctttagtgag aagataatta tcccctctat caaatacact
     5161 tacagtagca tcatcttctt gtatcttctt accatcagat tttatactag ctacattctt
     5221 atatttacta tcatgtttta catttttatc actaattcta gccttatact taacagtata
     5281 cgatttatct atatttccaa aatcaactga aatagagttt tcattctgtt caatattaaa
     5341 atcactcaat tcttttccat cacttgttac tacacttaca gaaccattta tcaatgtcaa
     5401 tccttcagga agctcgtcat acaaagtaat atcttttagc tcttcctgct tttcatttac
     5461 ttgtatactg taatttatgt ctttattttt aactggcata aataccgtag ttgattttct
     5521 tccttcaaca agcttcttaa gtgttccttt agcatctgga atatcttctt tatctccagg
     5581 ctttatttct acatctacat ctccacttcc actttctatt tttccagtag tttcaatatg
     5641 tatttttgca ttagtctctt catcaacttt tttaacatat gcttccaaac ctattttccc
     5701 ttgcattaca ctttgaccat taggtaattc tctaaatgta agtgtatact ctccattact
     5761 ttctgtttta tcaaagcatt cacttgtata atctaaatta actttattga atacatctgg
     5821 taccttaaaa gttattgaat cacctgcttg tattcctttt gatgttggag tataagttaa
     5881 attaaatgat atttttttca ttgattccgt ttctattgta ttgttattaa tttttacaac
     5941 aaatccatag tcttcatttt cttcattaag ttcttcttct aaaactttca ataaattttc
     6001 atcttctaat agattatctt cctctggagt tttttctgta tttttattgt ctagtggttt
     6061 ttcatcttct aaagcttgtt ctgacttttt attatctgtt gacttatctt cttctggtat
     6121 ctgttctggc tttttattat ctattggctt ttctccttct ggggtttgct ctggcttttt
     6181 attatctgtt gacttatctt cttctggagt ttgctctggc tttttattat ctgttgactt
     6241 atcttcttct ggggtctgtt ctggtttttt attatctgtt gccttatctt tctctggaat
     6301 ctgttctggt tttttattgt cgctttcctt atcttctcca gttgatgaat tagttgaact
     6361 actttgaatc tgactattct ctcctaattc tgaagcataa gatacagttg gatatattgt
     6421 agacaccaac atacttaaaa tcataattaa actaattaat aatgtctttc tattttcttt
     6481 cttcatataa atttcacccc tttaggttat ctataattta taatctaatc ttccattaat
     6541 atcgtatata aagtaatttt tactaaaaaa ttatacaaaa aaagctatga aaataaataa
     6601 atttatattc atagccggtt gcaccactga ctcagtatat ccataaggtg cccaataata
     6661 gaatatactt caacatctct ataatatttc ataaaaacaa ttaaaattgt tttatttata
     6721 ttaaaaatac aattatatac ttctattata ttatgcctct atactattgt ctatcctttt
     6781 tacatttttt acctttatta tgaggttaat taataattaa ataaaatcct tatctacaac
     6841 aattacacaa ttatattcag gatttatctc cttaactaat tttgttatat cttcttttaa
     6901 gtcatcctct gacatgtttt tggaaaattc ggaaggatta actactatat caaatattaa
     6961 attcttcttg cttccctttc caactattct aaaatcatgc atagatttta caaggctatt
     7021 tttcttaagt atttcttcaa gttctttttt ggcggacata acttctttat cttcaagaca
     7081 tattgggtcc atgtgaatta ctagatatat atctaatttt tcagatattt ctctctctgc
     7141 agtatctata atttcatgta tttccattat atctatgttg gatggaattt ctgcatgtat
     7201 agatgcaata catctaccga ctccatagtt atgaataatt aaatcatgtg tacctattat
     7261 atatttataa gataacaata gctctataat agaatttacc atttcttcat ctggtgcttc
     7321 tcctagaaga ggatttatag tatcttttac aagagagaaa ccagcataaa gtatcactaa
     7381 tgatactacg ataccaacat atccatctat aggaaaatta gtaaatctag ctacaataaa
     7441 tgatataact acacaacttg aagtaaatac atctcctaaa gcatcaagtg aaactgcttt
     7501 taaggctgaa gaatcaattt tatttcccat aaatttatta aaacgactca accagatttt
     7561 aatcattata gatgcaatta aaagtataag tggtattact tcaaatttta caggaattgg
     7621 atttactatc ctttctatag atgactttac aaattgaagt ccaaccaaca tcaccataaa
     7681 tgctactatt aatgctgata aatactctat tcttccatga ccaaaaggat gttctttatc
     7741 agctggctta cttgctaatt taaaacctat catagttatt gctgaagatg ccatatcaga
     7801 aagattatta aatgcatcag ccattatagc tatactagat gtgagcatgc ctatgaatac
     7861 ctttatcaca aataaaagta aattggataa tatacctact acaccagcta cataaccata
     7921 cttatttcta acatctgtat tttgaacgtt ttcgctatct ctgataaatg ttttgactag
     7981 gattttggaa aacatgaaac ttacctccct gccctttatt ttattaacaa attttataat
     8041 ctatatcttt atttatgata acataattcg tatataaaat tcttaaaaat cactctactt
     8101 taataaaata ttattctata tatccatttg gatacaaatt attgtatttt gtaaagtttt
     8161 tataatttaa tatctgttta atacattcta tgtcaagttt tacaaaaatt atttattatg
     8221 ataatatcat ttttcccaag tattacttag aatatgattt ttttgttttg tctgtacata
     8281 ttttaaattt a
//
LOCUS       DAEMAE010000163         8218 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEMAE010000163 DAEMAE010000000
VERSION     DAEMAE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.165"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(153..1820)
                     /gene="bclA2"
                     /locus_tag="KPH93_002799"
     CDS             complement(153..1820)
                     /gene="bclA2"
                     /locus_tag="KPH93_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG1689596.1"
                     /translation="MSDISGPSLYQDIGPTGPTGPTGPTGPRGATGATGANGITGPTG
                     NTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGANGI
                     TGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGATGLTGATGAT
                     GANGITGPTGNTGATGANGVTGATGPIGNTGATGSTGSIGATGANGVTGATGPIGATG
                     PTGADGEVGPTGAVGATGPDGAIGPTGNTGATGATGANGLVGPTGATGATGANGLVGP
                     TGPTGATGPDGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGAN
                     GAAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTGATG
                     ANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLLTFNSSF
                     INGTNITFPTSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVN
                     TLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(1999..2538)
                     /gene="hpt"
                     /locus_tag="KPH93_002800"
     CDS             complement(1999..2538)
                     /gene="hpt"
                     /locus_tag="KPH93_002800"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG1689597.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSAFTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2866..4173)
                     /locus_tag="KPH93_002801"
     CDS             complement(2866..4173)
                     /locus_tag="KPH93_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG1689598.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDVAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTENQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4585..6360)
                     /locus_tag="KPH93_002802"
     CDS             complement(4585..6360)
                     /locus_tag="KPH93_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1689599.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6357..6494)
                     /locus_tag="KPH93_002803"
     CDS             complement(6357..6494)
                     /locus_tag="KPH93_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689600.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6572..7141)
                     /locus_tag="KPH93_002804"
     CDS             complement(6572..7141)
                     /locus_tag="KPH93_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1689601.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7325..7960)
                     /locus_tag="KPH93_002805"
     CDS             complement(7325..7960)
                     /locus_tag="KPH93_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1689602.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2344 a         1642 c          946 g         3286 t
ORIGIN      
        1 cctattaaat attatcttat ttattggtat atggaatcat attgtttatt attttataat
       61 cttttcatca tataaaccta ttaaatatta tcttatttat tggtatatgg aattattttg
      121 tttattattt tataattttt tcatcatata aactattgta ttctataaac tgatacatat
      181 ccagctggag aagtacctgt cgtgcctgaa ccaaatgtaa aacttaaagt aacagctgct
      241 gctctagcat caactaaaaa acttaaactt acgcttacat ctgatgaagt aagcgtattt
      301 actgcttttg caataaagtt attttgagct actccattaa agttagtagt aattgacata
      361 aatcctgcaa gcgaaagtat ggcacgtata ccgaaagata cattgtatat ccctccaact
      421 gcaagattta tagtactact tgttggaaaa gttatattag ttccatttat aaatgatgaa
      481 ttgaatgtca ataatgtatt attccctaaa cttgaagaag atactgtaaa ttgtgcattg
      541 tttgctgcta acactcctgt tgctccagtc gctccggttg gacctgttgc tccatttgct
      601 cccgttgctc cggtcgcccc tgttgctccg gttgctccat ttgctcccgt tgctccggtt
      661 gggcctatcg ctcctgctac tccatttgct cccgttgctc ctactgctcc ggttgggcct
      721 gttgctccat ttgcccctgt tgctcctgtt gctcctgtag ctcctgctac tccatttgct
      781 ccggttggac ccgttggacc tattggacct gccgctccat ttgctcccgt tgctcctacc
      841 gctccggttg ggcctgttgc tccatttgcc cctgttgctc ctgtcgctcc agttggacct
      901 actgctccat ctgctccggt tgggcctgtt gctcctactg ctccggttgg acctattgcc
      961 ccatctggac ctgttgctcc agttggacct gttgggccta ccaaaccatt tgctcccgtt
     1021 gctccggttg ctccggttgg gcctaccaaa ccatttgctc ccgttgctcc ggttgctcct
     1081 gtatttccgg ttggacctat tgctccatct ggacctgttg ctcctactgc tccggttgga
     1141 cctacctctc catctgctcc ggttggacct gttgctccta ttggacctgt agcacctgtt
     1201 actccatttg ctcctgttgc tcctatactt ccggttgacc ctgttgctcc tgtatttcct
     1261 attggtcctg tagcacctgt tactccattt gctccagtcg ctcctgtatt tcctgttgga
     1321 cctgttattc catttgctcc tgttgctccg gttgctcctg ttagtccggt tgccccagtt
     1381 gctccggttg ctcctgtatt tcctgttgga cctgttattc catttgctcc agtcgctcct
     1441 gtatttcctg ttggacctgt agaacctgtt attccatttg ctcctgttgc tcccttgttt
     1501 cctgttggac ctgttattcc atttgctccc gttgctccgg ttgctcctgt cggacctgtt
     1561 attccatttg ctccagtcgc tcctgtattt cctgttggac ctgtagaacc tgttgttcca
     1621 tttgctccag tcgctcccat atttcctgtt ggacctgtta ttccattcgc tccggttgct
     1681 cccgtatttc ctgttggacc tgttattcca tttgctcccg ttgctccggt tgcgcctcta
     1741 ggtccagtag ggcctgttgg ccctgttggc cctgttggac ctatatcttg atataaactt
     1801 ggacctgaaa tatcactcat ataattaatc ctcctttttt aaagttagag taatactcta
     1861 taaatattga gtattactct atataattat atgaatcgac aatttcttat gtgataatgt
     1921 atctatttgc ttatataaaa aaacctcaac acgattaata aaaatcatat taaggcatcc
     1981 acaaaattat ataatagctt atctatcttc atcagtaaca ttgaatatat aaggaacatt
     2041 tctatagtaa tcaccaaaat caaacccata tccaaccaca aacttatctt ctatttcaaa
     2101 tccacaataa tctggcacta aatcgacttt tcttctgctt ggtttatcca aaagaacaca
     2161 gcactttaag ctagcaggat ttttagcttt tagatgtttt aacacaaaat ccatagtaaa
     2221 tgcagaatca gtaatatcgt ctacaaccaa aacatcatat cctgctatat cagtagtcac
     2281 atctgaaact actttaaccc ttccagtact ttcttcattg tttccataac tagatgtagt
     2341 cataaaatta actctaagtg gtaaatttat atttctaact aagtctgcac aaaatataaa
     2401 gctacctttt aatagagaaa ctaccataag atttttatct ttataatctt tttctataat
     2461 tgctcctaat tctcttaatt tatctttaat tttttcttct gaatacaata cttcccattt
     2521 ttttgtttct atatccataa aaagacctcc aattgtaata atccttctta attttataag
     2581 agattgctca ctttttcaat actcttattc aatattagtc aatattattg agtttttatg
     2641 ttcattcaac aattattaac aatgtatctt atattttaag tagttcatat atccacacta
     2701 aattacatac tatatatttt aaacaaaaaa agaaaaaact tgcactaaca agttttttct
     2761 attatacata aatttataca taatcatatt ttattagagg gtactaatta aatttattta
     2821 tgatattttt gttatacctc ataatacaaa acatattata aagtactagt caactttatt
     2881 catatcattc attacattta agattatctc atctgttata gacatacctt tatcacttac
     2941 tctacctaag ttttgtatac tttgctcaac acttgaacca actataccat ttagtggtgg
     3001 tacaaaacaa tcttgcttag ctattatagc actttgtata gctgcagatg ctgcagaaga
     3061 taattttaat gcacaaccag ctttcgcacc gtcacatatc attccactta agtcagctat
     3121 catattttct atagctcctt ctatttgatt ttcagtacca ttcataagcc aagagatacc
     3181 agcagttgct ccagtagatg cagcaacacc acatccacat acagcagata accttcctgt
     3241 ataattctta atataaccag ttactagatg agatatagct aaagcttttg ctagtctttc
     3301 atcagattgt gggaattttt tattataagc aacgattgga agaattgctg ttaatccatg
     3361 gtttccactt ccatttgaac tcataacagg catttttacc cctgccattc ttgcatctga
     3421 agctccagca gttaacatca ttgcataatt tataacatca ttacctaaaa gaccttcttc
     3481 tatagacttt ttaattccat atccgacacc aataccagtt ttttgtttta atccatattc
     3541 agccatctct tcattcattt ttactccatc tagtaaaaat tctatatctt taaaatcaat
     3601 ttcttctaca ttttttatta attcttgtat agttactgta tccattaaac tttcagcttt
     3661 ttctgctaca tcatttgaag ctgatacttt tggttcattg tctaataata cttctccatc
     3721 tttttctaaa aacgtaaaat tatcatgttt agttcttatt ctcactttag caatatggtt
     3781 ttttcctttt aacacaactt ctataaatac tttctctctt gtatcagcag gtgttattga
     3841 tagtggagta ttatccatat aatcctctgc tattttaact tcctcttttg ttagagtttc
     3901 taacacactt aatccatttt cagaatgtcc acctactata cctagagctg ctgcaatttt
     3961 taatcctaat ctttcagtac ctggtatacc tacgcacata ccatttttat agatactagg
     4021 actaactaac atacgatttt ctactatttc ttctcctaat aattcctttg cctttgcaca
     4081 tgctagagct aaagctactg gttcagtaca ccctactgct ggtttaactt ctgcctttaa
     4141 catttccact aattcttttc ttatatctct cattatatat aaacctccca attaagttta
     4201 gatttagtac tcaaataatt aattaaataa tttcttatct atatttatag caaaatacat
     4261 gccatatttt ttatatcttt ttatattttt agtcttatat tctataacca ttggtatgac
     4321 tgcttctatt tatgtatttt ttttatttgc atagagtatt tatattcttt aaaacggaaa
     4381 aacatatgac aatttcgcaa aaagacaaaa aaaagtctca aaatgagatt ctatatcatt
     4441 ttgagacggc tcaaacgtat tactttttaa tttataaaaa ccatttttta attcataaaa
     4501 aaagattatg caaaacactt atagcagaag tttaaagtta tgaatttaga aaatttaaag
     4561 tttatatatc tttttattat attactatga aattatattg tatttttcaa tttttctata
     4621 taatgtagct cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc
     4681 tttgtactta tttaaagctt ttattatctc aatcttctcc agttcatcaa gtggaattat
     4741 cctatcgctt ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac
     4801 attcattgtg attattgaag aagaagacat attcacacta tattcaataa tattctgcaa
     4861 ctctcttata ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat
     4921 gtcaataaca tttttatcta atttttttga atactcttta accatatgct tgattataag
     4981 tgggatatct tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct
     5041 ataatataaa tcttccctaa aggtgccatt ttgaaccatt ttttctaaat ctttatttgt
     5101 agccgcaata attcttacat ctattgaaac atttgattta cttccaattt tattaagttc
     5161 cttttcttgt aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc
     5221 atctaaaaat atacttccct tatgtgctac ttcaaacatt ccaagctttc cacctttttt
     5281 agctccagta aaagctccct cttcatatcc aaataattca ctctctaata aattgtctgg
     5341 tatagcagca cagtttacag ctataaaagg attatctacc ctattgctat ggttgtgtat
     5401 ggctcttgca aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt
     5461 tgatttagaa gctattaagg cttctttttt agaatttctt atagctacac tttctccaat
     5521 tatgttttcc aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc
     5581 tcttttatct ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt
     5641 aatgttgtat attcccttcg aatcatatcc gtacctttta taaaaaaaac tacaggattt
     5701 atccttctta aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt
     5761 tttgccagtt atactatctt ttatattgaa aagctcttta aattttgaat tatatttatc
     5821 aatttttcca tccacatcaa tagacacaac agctttgtcc atattgttta atagtgtctc
     5881 taattttttc ttttctaatt caagctcata agttttaatt tgagctttta atttattaga
     5941 tattaagtct gccatttttc ctaaaaagtt tataagacca cttttattat tttctataat
     6001 ttgtgcttgt tcttttgtaa aggctataag gcctataaca ccatgagaat cgccatcaca
     6061 tataatagga caacacacct ctgcaaactc tacacaagct ccacttttat agcattccct
     6121 acatatatca ctactctttg ggtcatcaat tagtattgta atctgttctg taagtgattt
     6181 tttaaaagcc gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc
     6241 tgcaatccta aataactcct tatttactat agttacatct atattgagta ctgatactat
     6301 agcttcagct atattttgaa tatcagattt tatatattct aaacctatca taatcatcat
     6361 tccttatttt tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta
     6421 tttaaagtaa attggattga cattagaatt acaaagagca taacaattat tattaattca
     6481 taatttattt ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt
     6541 atattatatc catatttata ttttaactca tttatcttct ttttcccaca taaatatatt
     6601 ttaatttatt cgactcttta atcataccgt caaagtttaa tcttgaaaat atattaaata
     6661 cttcatgtgg atagtgaatc tttgcaacag aaccacaaaa tttatgttta agatagttta
     6721 acttaacttt taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta
     6781 acactctttg tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa
     6841 ctattattat cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa
     6901 ctacattgca attttcatta tacactagag aataacagac atcttcataa ttactattct
     6961 ccagtatagt tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac
     7021 caacaagcaa tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt
     7081 catcagatgt atatttagct ttaactggct gtttatttat ctgacataat atgcttttca
     7141 caatgcttca cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa
     7201 tggcgagcaa ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt
     7261 ttaatatgat attataccaa atttattagg attttattca taaaaatcag tctatttttt
     7321 atgtttattc attgttattt tcatccaatt tctttatttt cgatatggat acttcatatg
     7381 ccattttttt gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc
     7441 taccccaaag ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac
     7501 cccaaactat tgatggtata taatcagatt tgttataagg tctgtttata gcaactaata
     7561 aatcggttat ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc
     7621 catctaaaaa tatactatta gggtctttat tttcctcatc tatgcttttt atttctctga
     7681 caaacactgt aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt
     7741 ggcctacgac attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg
     7801 atacagtcat tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat
     7861 tataaaattt ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc
     7921 ttaagtttac tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg
     7981 ttaattctta tatttctggt atcagcttat ataataaata tatttataag ggatgtattt
     8041 tattacaaat ctttgattgc tgtttgaaag tttttattta gttataaatt tcatttaata
     8101 tttaactttt ataattataa aattattatc tttttgtacc acatatacta attaaactac
     8161 ctatttcagt tttggattaa ttataaaatt cctattatat aattaataag tcaaatat
//
LOCUS       DAEMAE010000164         8217 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEMAE010000164 DAEMAE010000000
VERSION     DAEMAE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.166"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..727
                     /locus_tag="KPH93_002806"
     CDS             56..727
                     /locus_tag="KPH93_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689603.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQLIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKVCLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDENIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            729..1637
                     /locus_tag="KPH93_002807"
     CDS             729..1637
                     /locus_tag="KPH93_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689604.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELSLMLNQILEGKEVTYP
                     DTKDTRASKISQQVKKIKDMIEIEVEQSKLEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRVNLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1712..2398
                     /locus_tag="KPH93_002808"
     CDS             1712..2398
                     /locus_tag="KPH93_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689605.1"
                     /translation="MSILVTNNLVKHYGKDETKVKALNGVSIEIERATFTAIVGTSGS
                     GKSTLLNIIGGLDSPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2395..4950
                     /locus_tag="KPH93_002809"
     CDS             2395..4950
                     /locus_tag="KPH93_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1689606.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     AYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNTKK
                     RILESVRHSNMTKVLSHKYMTQKKGLFVGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAIQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGVIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     VGLSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFSLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINAPLYIGMIISNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(5144..6343)
                     /locus_tag="KPH93_002810"
     CDS             complement(5144..6343)
                     /locus_tag="KPH93_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG1689607.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6462..7349
                     /locus_tag="KPH93_002811"
     CDS             6462..7349
                     /locus_tag="KPH93_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1689608.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENGKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7539..8096
                     /locus_tag="KPH93_002812"
     CDS             7539..8096
                     /locus_tag="KPH93_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689609.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
BASE COUNT         3244 a          960 c         1258 g         2755 t
ORIGIN      
        1 gtatgataat gcaagaaaat aattatttat ctaaaaaatc taaaggagag taaagttgaa
       61 cttattaatt attgaagatg atataaactt aaatgaaggt cttttttatg catttgagaa
      121 tgatggattt aatgtattta aggcttatac aaaacaagaa ggattaaata tatttaacag
      181 taaaaatata gattttataa tcttagactg caatcttcca gatggagatg gatttgatgt
      241 atgtcaacta ataagggaaa agtcagatat accaattata atgcttactg caagagactc
      301 agaaattgat gaagtaaaag gattagaaat agggcttgat gattatatta caaaaccatt
      361 ttctttatct gtattaaagg caagagtcaa agtagcaata agaaaaaaat cgaataataa
      421 agtaatatat tccaatggaa taaaattgga ccaaaagcta ctaaaagtat ataaaaataa
      481 agtatgtttg gaacttagct ctgtagaata taagcttcta agttatctaa ttgaaaataa
      541 aggtcaaata ttattaaagg aacaaatttt acaccatatt tgggacagcg aagaaaatta
      601 tgttgatgag aatattgtgt ctgtaaatat aagaagactt agagtaaaag tagaggatga
      661 cccatctaat ccaaaatata taaaaacagc ttatggaatg ggttatttat ggaatgaggt
      721 agaataagat gtttatatta gcctttataa tatgtacagt tgttatattt atagtatcta
      781 ctaaaataaa tcaaaaaaga tatgatgaat taagtttaat gttaaatcaa atattggaag
      841 gtaaagaagt tacttatcca gatacgaagg atacaagagc gtcaaaaatt tctcaacaag
      901 taaaaaaaat taaggatatg atagaaatag aggtagaaca gtcaaagtta gaaaaagaag
      961 cgattaaggg tttaatatca aatatgtcac atcaattaaa aactccactt tcaaatataa
     1021 ccatttattg tgaacttctg gagaatataa atatttccac attacagaag aaagagtttt
     1081 tacaaaagat gaaaaatgaa acttttaaga ttgactggtt gttacaatcc ctatttaaaa
     1141 tgaccaaatt agaggatgga gttatagaat ttgaagtaga agaactacta attaaagata
     1201 ctcttattca gagtatcagt actatcttta ataaagcaga agctaaaaat ataagggtaa
     1261 atcttgaacc attttcagat ataaagttag ttcataataa aaagtggacc atagaagcaa
     1321 ttgtaaatgt tttagaaaat gcaataaaat actcaccatc agatagcacg attactattt
     1381 ctgtaataaa aatggaactg tatacgaaga ttactattaa agatgaagga ataggtattg
     1441 atagcagaga actaaatgat atttttaaaa gattttacag aagtaaaaat gtggaaaacc
     1501 aaaatggtac agggattggc ctatatctta caagattaat tttagaaaaa gaaaatggaa
     1561 acattatagt agaatctaaa ctaggaagtg gatgttgttt tagcatcttc ttacagaatt
     1621 gtaagagttt aaactaactt tgtaggatga gtgtaattat cgtcacttac aatattttat
     1681 agaacatata tctattgaaa ggagaaatta aatgagtata ttagtgacga ataatttagt
     1741 aaaacactat ggaaaagatg agactaaagt aaaagccttg aacggagtaa gtatagaaat
     1801 tgaacgagct acatttactg ctatagttgg aacttctggt tctggaaaaa gtacattgtt
     1861 aaatattata ggtggattag atagtccaac ctcaggtgat gtaataatta aaggaaagaa
     1921 catatctaaa ataggaaaaa aagatttgac agtatttaga agaagaaata taggatttat
     1981 atttcaaaac tacagtttaa tgccagtatt aaacacttat gataatatag ctcttcctgt
     2041 tacattagat aaaggaaacc atatagacca tgaatatatt gaaatgctga tgaagacatt
     2101 gggaatttgg gataagcgtt taaaatttcc aagcgaacta tctggtggtc aacaacagag
     2161 ggtagcaatt gctagagctt tagcaaataa acctgcactt atattagcag atgaaccaac
     2221 aggaaatctt gatagtaaaa ctacaatgga agtggtatgt ttattaaaag aaagtagtgc
     2281 aaaattccat caaacgatac ttatggtaac acacaatgaa aatatagcac aagtatgcga
     2341 ctcaattatc catattgaag atggagttgt ggtgaatact ggtggtgaag tactatgatg
     2401 tctctagtaa aattttcaat tcaatatata aagcattata aaaaacaatc tatatctata
     2461 gttttgagca tagtactatc agtagcatta ttgacgggaa ttggttcttt agttcatagt
     2521 gcagacaaaa gtagaattga aaagataagg gaagatagtg gagattatca ttatttctat
     2581 aaagtagata aggaacaact taataaaatt aaagaaaata agaaatcaaa ggaatacact
     2641 gtagatagac ttggtgttac aaaacctaaa aatcagatag atgaaccttt tattatagaa
     2701 tttttaaatg ctgattcttc atatttagac atgacaggta gaaaattatt aaaaggaact
     2761 cttcctagta aagatggaga gatagtactt gacacatatt ctctaaacaa tttaaatgta
     2821 aacaaagaaa ttggaacaga aatcaattta gatgggaaaa tctataagtt atgtggtatt
     2881 ttatcaagtc tttcaaaccc agaaaccgct atggagggat ttgtaaacga ttatttacag
     2941 cctaaagaaa aggattctta tatagtttat gtaaaatatg atgaaaataa aaatattaaa
     3001 aagcaaaatt ataaatttat gaaaaaattc aacatatcaa aagaaaaagc ttatataaac
     3061 tggcctcttt cagttgaatt tggagtaaaa ccaccaaaaa atatagaaaa atataatata
     3121 tttgagttct taaaaaattt agaattaaat acaaatgcaa ttatattact tgttggagtg
     3181 tttggtgcag cagcagtata tagtattttc catgtttcaa tacttcagag aatatcacaa
     3241 tatggggttt tagaagtatt aggtgctaat aataaacaac ttttatttct actattttta
     3301 gaattatttt tactttttat tgttggattt cctataggtt gcttcctagg tattggagta
     3361 gcgtctacaa tatatgaaca atttccacat atatttttga gtagtgatat tgttccagga
     3421 gcatttttta taagtgaaaa atctatttat ttaggatttt tatttatctt attgttgtta
     3481 attttaattg ctgtacgtgt tgtacatcag ttgaataaat actctagtat agaatctatg
     3541 aaaaacttta agattactaa tacgaaaaag agaatattag aaagtgtaag acattctaac
     3601 atgacaaaag tattatctca taaatacatg actcaaaaaa aaggattgtt tgttggaatt
     3661 ttgttttcat tatcacttgg tggaattata tttctatgtt ctagttactc tatacagctt
     3721 actaaaaaca ataatgaact tacaatgaaa gctgatgatg gtttaaattc tgactaccaa
     3781 ataactatgc aaacaacaga ttttgatgta ggtataccaa aagataaaat ttcaagatta
     3841 aacaatgttg agggaatatc gaaagtctat ccagtaagat atttatttgg agctatacaa
     3901 attaaagaag aacaactatt ttggaaaaat ttctttaaac ctcttgaaaa agattacagg
     3961 ataaagactt tttttgatgg tatttgtaca aagcaaaatg aaggtggcta tcttttaaaa
     4021 acaaatattt atggttataa caatggtatg ttagaaggat taaattctta tattatagat
     4081 ggggtgatta atacccatga tatggttaaa aataataagg ttatagttag actacctatg
     4141 gatggaactg ggatgtatga tgcagttgat ataaatccag gagatacaat aaaagttaaa
     4201 gtgccaaaga caatgaaacc aacagatgaa attgttaaat ttaaagaaga aaataagaat
     4261 gattatacaa caaaagaatt tgtagttgct gctactgtaa aacgtgtgat ggcaaataac
     4321 atatatttca ttggtgatta tggtatggat atagttatga cgaatggaca aatgaaaaat
     4381 aactttgata tcgaaaatta taattcagta agtataaaaa agacagaaga tagtaatagt
     4441 gtgggattat ctgagaacat aaaaagtgtt gtaagtgatg taaaaagatg tatcgtaaca
     4501 gattatacta ttgctattga aaaaaataat acctacttaa atcaaaaact cttatttata
     4561 tatggtattg tatttgtact tcttgcaata agtttattcc atataatcaa tacagtaagt
     4621 tacttgattt tttcaagaag acatgagttt ggaattttaa gagctatggg tattacagat
     4681 aataagtttt cacttatgat gataagagaa gggtttttat atggattcta tgcaagtatt
     4741 ataatggtaa ttggaagtgt tattggacaa tttatgattt attttatggt aaaacgtgta
     4801 tatctatata taaatccaat tcttaaaata aatgcacctc tatacatagg tatgattatt
     4861 tcaaatatca ctattagtat agttgctgta attattccag taagacaaat tttgaagagt
     4921 gatattattt cagaaataaa taagagttag agttatataa atttttaaga ttatataaat
     4981 ttctgacaat cttgtttaaa ttatttaaat atatcttatc tgtgaacatt aaataaacct
     5041 cttaatagat ataactcttg aaaagtcata tctattaaga ggttttaata aattttcatg
     5101 ctaatatata taatccaaaa aatttaaatc ttattaaaac atactatttt gtatagttat
     5161 cgaaatatcc ttgtatgtaa ataatagggg tacccttatc tccacttcca gatgttaaat
     5221 ctgctaaaga acctataagg tctgttaatc ttcttggagt agtaccttga gaaaccatgt
     5281 ttcctgttaa atcatcagat ttattatctt tttcaactat gtattttgaa attgcttctt
     5341 ttaactcatc accagataag tcagcaaaat cattatctgc taagtatttt aattttactt
     5401 cgtttggagt tccttctaat ccttttgtat atgcaggaga tactactggg tcagctaatt
     5461 cccatatttt acctacaggg tctttaaaag ctccatctcc ataaaccatt acttctacat
     5521 ctttccctgt gatttctttt atattacctt ggattttgtt tacaacttca tcacaattta
     5581 tagggaataa ttttactgtt tcttctgtag ctttgtttga acctaaaagt ccatactggt
     5641 cattgtaacc actaccatct acacttgctg tcattatatc atcaagtgaa tatacttttt
     5701 tagctccatt ttgtcttaaa attctctttg ttctttgtct tgtatgtata tcacaagtta
     5761 aaatacttgt tgtatagtta agaatagttt ttggattgtt tgcaaatact atttctacct
     5821 ctgcaccaca atccttgatt aaattcttat aataatcaac ataatctaca ccagtaaatg
     5881 tatgcttgtt atatccaaat agttctctgt atttttcttc tgttaaaaca tctgaccaag
     5941 gatttattcc cttatcatca agttcatcaa tacttattaa gtgatttcca acttcatcag
     6001 aagggtagct taacatcaat acgacttttc tacatccttt tgcaattcct tttaaacata
     6061 tagcaaaacg atttctactt aaaattggga atataacccc aacagtatca tctccaaatt
     6121 tatcttttac atcttttgca atattgtcaa cgtgagcata atttccctgt gctctagcta
     6181 caacagcttc tgtaacggca attacatctt tgtccctaac ttcaaagttt tcactttttg
     6241 acgcatttaa aacagaatca acaactattt taactaagtc atctccttgt cttataatag
     6301 gtgctctaac ccctcttgaa acagttccaa ctaatctatc cattatctaa aatcctccta
     6361 aagaattaat attgtcatat tagtaccatg tatattataa ttcaaatata attataagta
     6421 aaataaataa tactaatatt tgacataagg agtaattata tatgaatata aaacttgaac
     6481 tttataaggt ttttaatgca gttgtaaata ataagagttt ttctcttgct gctaaggaat
     6541 tgtttatgtc acagccagct gtaagccagt caataaagca attagaagaa caattagata
     6601 ctttactatt ttatagaaac aataagggag taaaattaac tccagagggg aaaattctaa
     6661 gtgaacatgt cactactgca ttaaagttaa tatcatcagg agaagataga ataaataaat
     6721 ttaaaaaatt agaatacggt tctctgaaaa taggagtagg agatacagct gcacgttttt
     6781 tcttgttaaa atatttagaa atttttcata agaagtatcc acatatacat gtatctacaa
     6841 taaatagaac gagtagagaa cttatatctt tactcaaaga tgggaatata gatatagcaa
     6901 ttataaacat gccaatagaa gatgatacac taaatattgt tgagtgtatt gaaatacatg
     6961 atatatttgt atgtgcaaat gattacatag aatacaaagg taggaaaata tcattagaag
     7021 aattaaatac attgccttta ataatgctag aaaataaagc aaattcaaga ctttatgtaa
     7081 atgaatattt tttatcaaaa ggtataaaac taaacccgga tatagaattg ggttctcatg
     7141 aattgctttt agaatttgcg tatataaatt taggagtgtc ttgtgttata gaagaattta
     7201 gcatagatta tttggaaaat gggaaactgt ttaaattaga tataaaagag ccaataccta
     7261 agagaaatat aggttactgc catttaaaag atatatcttt atccttagca accaaagaat
     7321 ttttaagcat gatatctaat aacatataat ttagaataca tatttcacaa gtaatttaat
     7381 ataaaaatag agataagttc gtaaatttga aagattcata tagtaattag tttttattaa
     7441 aaataaaaat aaggttaatt tgagaacatt tatataatta atatcgtatt ttaaatatga
     7501 acagtatatt tagattaatg tataaagggg gattagaaat gtctgattct gaatatcctc
     7561 aaatgaaaac cttagaaact agaaattgta tactaagacc tgcatcatta gatgatgtcg
     7621 aagatttttt tgactgttat aaaaataaaa tagtagtaaa acatttacca tttagtgagc
     7681 ataaatcttt agaagataca agaaagttta taaaatcatt ttttctaaat ccctataaaa
     7741 aagggaaaat tggacacttt gctatagtat ataaaagaga caataaagtt attgggaata
     7801 tggggtttaa taatataaat cctaaagcat tagaagcaga aataggtata tgtataaatc
     7861 caagctattg ggggcatgat tttgctactg agataacaaa aagagtaata caatatggat
     7921 ttagagaact aaacttaaat aaaataattg caataaccta tgaagaaaac caaaattcta
     7981 caaaatcatt agatttatta ggatttaaat gtacaggaaa gtatatcaaa aaaattcata
     8041 ctggaaatac tgtaaaaaat gttccttgtt atcaatatga attgaaaaaa caatagatat
     8101 aatcatttaa tatttatgct agttatttaa taatgattgt tttctaaata aaatttcaat
     8161 aaagtggttg cctcaaaata tagataagcc tgttttttat tgttaaaata ctgaaaa
//
LOCUS       DAEMAE010000165         8170 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEMAE010000165 DAEMAE010000000
VERSION     DAEMAE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.167"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(335..1150)
                     /locus_tag="KPH93_002813"
     CDS             complement(335..1150)
                     /locus_tag="KPH93_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1689610.1"
                     /translation="MKTGKYFETVSGIEIHYIDEGKGKPVIFVPGWTFSCDVFEAQVN
                     RFSSEYRVIAVDPRAHGRSTVTDIGVDHTTHAQDIVKLIKHLGLNDVILVGWSFGAYE
                     TWGVIREIGIEHIKGIFNIDMSPKAISTNLGDWVEDSVEALCETIQVILTPQGFKEVL
                     KEYTNEVMIQKTLSEEEMEFIMSCSTMPYYLASAEYAIGVTGDYRKEAKLADESEGCH
                     TEFMIAEHWQDVAVPYMKKLCPNTKLHILGGHMAFWEYADEFNAILSEFLKKC"
     gene            complement(1370..2116)
                     /locus_tag="KPH93_002814"
     CDS             complement(1370..2116)
                     /locus_tag="KPH93_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1689611.1"
                     /translation="MKIAILTDLIVKWLKQGGATVYTGKVDKSNNYLAEQCQIANRQN
                     VDVAIQIHFNADHTTLDKMGTETIYKTNNGKVYADRVNTKLATVFKNRGAKSDVRGLF
                     WLSHTKAPAILIEVCFVDSKADTDYYIRHKDIVAKLIAEGILNKSINSNSTESGGNNN
                     MDKFDTAIVYSGETDKAIATIMSFYISNSTIVDIKDYKSYMCRNVFVIEGGATEGIKK
                     YPDKYTNFMGADRKETFKLVLEYLKNKKLL"
     gene            complement(2116..2373)
                     /locus_tag="KPH93_002815"
     CDS             complement(2116..2373)
                     /locus_tag="KPH93_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG1689612.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGANQTYKQLKEENK"
     gene            complement(2393..2623)
                     /locus_tag="KPH93_002816"
     CDS             complement(2393..2623)
                     /locus_tag="KPH93_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG1689613.1"
                     /translation="MNEELFKENLKRHEVTINKHNDEIDELKVANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAIQRGIF"
     gene            complement(2681..4228)
                     /locus_tag="KPH93_002817"
     CDS             complement(2681..4228)
                     /locus_tag="KPH93_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689614.1"
                     /translation="MATVYEFNYTGSEQSITLKPGKYKLECWGACGGTVDASDWTDCT
                     KGGYSKGEIVFKKRTNLQIYVGQSGYEKVTEGSSLTRSGFNGGGVAGKVTTGKFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGGIGVGGGSGYALTKDSYKPAGYIP
                     TSEYWLENVVMESGGNTTKSDGYAKITLLKSLPFLTVSSYNSTQITFKADHTNPTLLT
                     KIEVFIDDILKETIITDLTTEKTINYILEYNALHTLKIVVTDNANTTAEKVVSISKNI
                     MPLGDDASLQDIASKVKEMKHGLINGKTSIINVLSLKNIESSLNNSLVELSEKVKESF
                     DSSDTSLQDLINQLTQANNTISQLNSKYKYSSGTFTYYTGAYPNGINIGFRPDVISYT
                     VINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNHYGITFTDNGWIPKGPVNNGVYS
                     YIAVKCC"
     gene            complement(4363..4545)
                     /locus_tag="KPH93_002818"
     CDS             complement(4363..4545)
                     /locus_tag="KPH93_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689615.1"
                     /translation="MNINNIVVRIVSERILNRGLNPLKNRPFELDDVTNIEYRKAVED
                     YIIEHSGVVEGTEPTK"
     gene            complement(4545..4837)
                     /locus_tag="KPH93_002819"
                     /pseudo
     CDS             complement(4545..4837)
                     /locus_tag="KPH93_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4851..6344)
                     /locus_tag="KPH93_002820"
     CDS             complement(4851..6344)
                     /locus_tag="KPH93_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689616.1"
                     /translation="MATYDVDRGVKLREGEHTDEEIFLRACSYQTGGRTYYGTFEVGN
                     EIKVFELETRLYTATTTNVRYYSTSGSEVMVRDVVMRQNVTAMFVAKPTINIKDNLGI
                     ISDACEIEYTISDGFPELRYNIVYKLNNDIIGQIVNTVDSKYKISLTDEYLSKLSHNS
                     TNHIVIEFNDFNNRNMLTKTVIFTKGNTKPKLNITSYNSTTTIFTAIDTDNNLSKIEW
                     FIDDVLKETITTDLYLEKIINYELTDNAVHTLKIVATDAENATVEKVLSISKEIMPFQ
                     SDASLSDISTKLAEIGEGFKNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSD
                     ASVQDLMNQLTQANNTISQLDSKYKYASGTANARENSSLIACIYDPNTSHTVEETSPY
                     WLDLNGIGFIPDIFFAECEYEPNSDAFYKYFVFAIKNTFSISNNTGFVVNITFNKEYG
                     DRSFKLRGDLYTLGKRHVSMDNTGVRVPALNTLNNLRAYKWHAAKFK"
     gene            complement(6360..6707)
                     /locus_tag="KPH93_002821"
     CDS             complement(6360..6707)
                     /locus_tag="KPH93_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689617.1"
                     /translation="MQDLETKIGTVNIKIDTTKTELTSNIETTKTELIGKIGDTTQLT
                     TTDKTNIVSALNEVKTSVDSIETTADKTSIKDTDNLFESDNVEGALKEVMQEVKGNRS
                     SIISSINSNLIPM"
     gene            complement(6695..7222)
                     /locus_tag="KPH93_002822"
                     /pseudo
     CDS             complement(6695..7222)
                     /locus_tag="KPH93_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
     gene            complement(7217..8107)
                     /locus_tag="KPH93_002823"
                     /pseudo
     CDS             complement(7217..8107)
                     /locus_tag="KPH93_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
BASE COUNT         2519 a         1514 c          967 g         3170 t
ORIGIN      
        1 tcttgaatac atagcaatag gtttattcac aagaagatta gatattacaa tattagcaat
       61 aatattagta gccattataa caggggtatt ttactattta attagaaaga aatatataac
      121 tctgtaaaaa ctaaaatcaa atatttagtt gacaccattt tactaatgta tgctatggag
      181 tttttactca ctttactata taattcggcg tataagcact atacattata taatttatac
      241 aaagatagtc catgataata tgagctatct ttgtatgaat agtctataaa ccagttctgt
      301 attttacaat tttaatacta ccttcccaca ttcactaaca ttttttcaaa aattcagaca
      361 atattgcatt aaactcatca gcatattccc aaaaagccat atgccctcct aaaatatgga
      421 gtttagtatt cggacagagc ttcttcatat acggtacagc tacatcctgc caatgttcag
      481 caatcatgaa ttctgtatgg catccttcgc tctcatctgc cagttttgct tcctttctgt
      541 aatctccagt tacacctatt gcatattctg ctgatgccaa ataatatggc attgtagaac
      601 aactcatgat gaattccatc tcttcttctg aaagtgtttt ttgtatcata acctcatttg
      661 tgtactcctt caaaacttct ttgaatcctt gaggtgtaag aattacttgt attgtctcac
      721 ataatgcttc cacactatct tccacccaat cgcctagatt tgtagaaata gcttttggtg
      781 acatatcaat attgaaaatt cctttaatgt gttctattcc aatttcacgg attacgcccc
      841 atgtttcgta tgcaccaaaa gaccaaccaa caagtattac atcattaaga cctaagtgtt
      901 tgataagctt tacaatatcc tgtgcatgtg ttgtatggtc aacaccaata tctgtcacag
      961 tagaacgacc atgtgctctt ggatcaactg cgattactct gtactcagaa gaaaatctgt
     1021 tcacctgcgc ttcaaagaca tcgcaagaaa aagtccaacc tggaacaaat ataactggtt
     1081 ttccttttcc ttcatcaata taatggattt caataccaga aactgtttca aaatatttac
     1141 cagtcttcat tttaactcct ccattcttct atccaaaaat aatataccta cttacaaata
     1201 ttgctaattt cagtttatca tattttgtca ttttaaatca attcaaattc ttactaaaaa
     1261 gatacacaag aaaattttta aaaaaacaca aaaaacttct gtatatgatg gccactacac
     1321 taatttcatg aataacaagt tattacctgt tgtatctata aacctttatt tatagtaatt
     1381 tcttattttt taagtattct aatactaatt taaaagtttc ttttctatct gctcccataa
     1441 agtttgtgta tttgtcggga tactttttaa ttccttctgt tgctcctccc tctattacaa
     1501 atacatttct gcacatataa ctcttataat cttttatatc tactatagta ctattactaa
     1561 tataaaaaga cataatagta gctatagcct tatcggtttc cccactgtag actatagctg
     1621 tatcaaattt atccatgtta ttatttcctc cactttctgt actgtttgaa ttaatactct
     1681 tatttaaaat accttctgct attaatttag caactatatc tttatgtcta atataataat
     1741 ctgtatctgc tttactatct acgaagcaca cttctattaa tatcgcagga gcttttgtat
     1801 gactaagcca aaaaagccct cttacgtctg attttgcacc tctgttttta aatactgttg
     1861 ctaatttagt atttacccta tctgcataca ctttaccatt attagttttg tatattgtct
     1921 ctgtgcccat tttgtccaag gttgtatggt ctgcattaaa atgtatttgt atggccacat
     1981 ctacattttg cctattggct atttgacatt gttctgctaa atagttatta gacttatcta
     2041 cctttccagt atatacagta gctccacctt gtttcaacca tttaacaatt aaatcagtta
     2101 gtattgctat tttcattatt tattttcctc ctttagttgt ttataagttt gatttgctcc
     2161 tattgcaact ccccaacata aaataccttg taaaattgat gaagggttaa atcctagcat
     2221 ccatattgaa aatactattc ctagcacaag caatataatt ggaatgtatt tattatctaa
     2281 ttgtttatat ttcttaaatc caaatcctaa tacattaaga gcaactacta ataaaagcag
     2341 ttgctctggt ataaaactta ttaaattatc catctcttat cctcctaatt aattaaaata
     2401 ttcctctttg tattgcaaat ataaagaacc ctactagtgt tgtaatcatt gttccaatta
     2461 gccactttaa catacttgta agtgaattta gattctcaca caatgctttt aactctgctt
     2521 tagactctat atttgctact tttaattcgt ctatttcatc attatgttta tttattgtta
     2581 cctcatgtcg tttcaaattt tctttgaaaa gttcttcatt catgaaaacc tccttattta
     2641 atagtaaata gggcttagaa gttatctaag cctttgataa ttaacaacat ttaactgcta
     2701 tataactata cacaccattg ttaactggac ctttaggtat ccatccattg tcagtaaaag
     2761 ttattccata atgattatga ttaacatcaa gatatttttc tccagttgta taattataaa
     2821 aaagatagaa taaaggtgtg tatacacaat aatatttttc ttctacagca ttgataacag
     2881 tgtatgaaat aacatctggc ctaaaaccta tatttatccc attaggatac gcaccagtat
     2941 aataagtaaa agttccacta gaatatttat atttagagtt taactgtgat atagtattat
     3001 tagcctgtgt taactggttt attaaatcct gcaaactagt atctgaacta tcaaaggatt
     3061 cttttacctt ctctgacaat tctactaaag aattgttcaa acttgattca atattcttta
     3121 atgataatac atttatgata gaagttttac catttattaa gccatgtttc atttctttaa
     3181 ctttacttgc tatatcttgt agactggcat cgtcacctaa tggcattata ttcttgctta
     3241 tacttacaac tttttctgct gtagtattgg cattgtctgt aacaactatt ttaagcgtgt
     3301 gtagtgcatt atattctaat atatagttaa ttgttttctc tgttgttaaa tctgttatta
     3361 tagtctcttt taatatatca tctataaata cttctatctt agttaacaat gtagggtttg
     3421 tatggtcagc tttaaatgta atctgtgtgg aattataaga agatacagtt aaaaatggta
     3481 agcttttaag taatgttatt ttagcatagc catctgattt tgtagtattt ccacctgatt
     3541 ccataactac attttctagc caatattcag aagttggtat atatccagca ggtttataac
     3601 tatctttagt tagtgcgtaa ccacttccac cacctactcc tattccacca taacctcctt
     3661 ttcctaacga accatggtat tcttctgtat cgtaacttgt tccaccttgg tattgagaac
     3721 caccaccaca aaaatctctg tcacgaccaa ctccattaac acctacataa ccaccaccat
     3781 gaccaataga acgagcagaa gcaaaattat ttttcatacc tccgccaccg cctgcaacaa
     3841 gtatgcgtga aagcaaactt tcagtgttac cccaagttgc actaggatga taaagtctta
     3901 tatcagttgc tccaccaccg tatttagaat aagcgaattt accagtagta actttgccag
     3961 caacgcctcc accattaaaa ccacttctag taaggcttga accttcagta actttctcat
     4021 aaccagattg accgacataa atttgtagat tagttctttt tttaaataca atttcacctt
     4081 ttgaataacc accttttgta caatcagtcc aatcgcttgc atcgacagta ccaccacaag
     4141 caccccagca ttctaattta tattttcctg gttttaatgt aatactttgt tcacttcctg
     4201 tataattaaa ttcataaact gttgccattt aatccaccta tttcttaatt tatggtttta
     4261 ttgtacctaa ttttaaaact ataaacgtct acaaagtgtg gatgaagtgt agacaaaatg
     4321 taaatgaatt tatatatttt aagaaataaa aaaggacatt acctattttg taggttctgt
     4381 tccttctacc actccactat gctctataat ataatcctct actgcttttc tgtactctat
     4441 gttagtcacg tcatctaact caaaaggtct gttttttaaa gggtttaatc ctctgtttaa
     4501 aattctttct gatactattc ttaccacaat attatttata ttcattataa taatcctcct
     4561 accttttcat tttctgcaat tagtaattga ttttctagct cttgtattct cttttcttct
     4621 tcgcttaata ggttggaata tcttttaaaa taggttcttt tgttattgga tttatagatt
     4681 ctatatactg tttactatag tctatatttc catattcaac atcaatataa tgtaattctg
     4741 ttattgtatc atgctctaat atatctcctg ttgcttctcc agtttgtaaa agtattttac
     4801 cagtttggtc gtgaattatt ctatttgctc tattcatttt atcatctcat ttatttaaat
     4861 tttgccgcat gccatttata tgctctaaga ttatttagag tatttagagc aggtactcga
     4921 actccagtat tatccatgga aacatgtctt ttgccaaggg tatataaatc tcctcgtaat
     4981 ttaaaagatc tatcaccata ttccttgtta aaagtaatat taactacaaa cccagtatta
     5041 ttagaaatgg aaaaagtatt tttaattgca aaaacaaaat acttataaaa agcatctgaa
     5101 tttggttcat attcacattc agcaaaaaat atatcaggaa taaatccaat tccatttaaa
     5161 tcaagccaat aaggacttgt ttcttcaaca gtgtgagaag tattagggtc atatatacat
     5221 gcaattaagg aagaattttc tctagcatta gcagtcccac tggcatattt atacttagaa
     5281 tctaactgtg atatagtgtt attagcttgt gttagctgat tcatcaaatc ctgcacacta
     5341 gcatctgaac tatcaaaact tgttttaatt ttctctgata actcaactaa ggtattattt
     5401 aaacttgctt ctatgttctt taatgctaaa gtgtttataa tacttgtttt accattctta
     5461 aacccttctc caatctctgc taacttagtt gatatatcac ttaaactagc atcagattga
     5521 aatggcataa tctctttact tatactcaaa accttctcaa ctgttgcatt ttctgcatct
     5581 gtagcaacta tttttaatgt gtgtactgca ttgtctgtaa gctcatagtt gatgattttc
     5641 tctaaatata aatctgttgt tattgtttct tttaatacat catcaatgaa ccactctatt
     5701 ttagataaat tattatctgt atctattgct gtaaagattg tagtagttga gttataagaa
     5761 gttatattta attttggttt agtattacct tttgtaaata taactgtttt agttaacata
     5821 tttctattat taaaatcatt aaactcaata acaatatgat tagttgaatt atgacttaat
     5881 ttagataaat attcatctgt aagacttatt ttatattttg aatcaacagt attaacaatt
     5941 tgacctataa tatcattatt caatttataa actatattat atcttaattc aggaaaacca
     6001 tcgcttatag tatactcaat ctcacaagca tcacttataa tacctaaatt atcttttata
     6061 ttaatagttg gttttgctac aaacattgca gtaacgtttt gacgcataac aacatctcta
     6121 accataacct cagaaccact agttgaataa taacgcacat tagttgtagt agcagtataa
     6181 agtctagttt caagttcaaa tacttttatt tcgttgccaa cttcaaaggt accataataa
     6241 gtacgtccac cagtttgata tgagcaagca cgaagaaata tttcttcatc tgtatgttct
     6301 ccttctctta atttaacacc tctatcaacg tcataagtag ccatttaacc tcctcctttc
     6361 tacattggta tcagattact gtttatactt gatataatac tacttctatt accttttact
     6421 tcttgcatca cttcttttaa tgctccttct acattgtcac tttcaaataa attatctgta
     6481 tcttttatac ttgttttatc tgctgttgtt tctatactat ctacactagt ttttacctca
     6541 tttaatgcac taacgatatt tgttttatct gttgtagtaa gttgtgttgt atctcctatt
     6601 tttccaatta actctgtttt agtagtttct atgttgcttg ttaattctgt tttagttgta
     6661 tcaattttaa tattaacagt acctatttta gtttctaagt cttgcatatc tttgagtgtt
     6721 gcaaagttaa tacatcaatt tctccataag tttctatatc tggtggactc caagggtata
     6781 tagttatttc ttgacccatt agggttgtta taccaaaatt catataattg tctttgctta
     6841 taagcactct agtgtaatct agtgtcatat tgcatggctt aatattactt acaaaagaat
     6901 gaacttcctc aaaccaatct tgatttctag catcactctc aaggtgtatg ttataagtag
     6961 cattattaat agttaattca taattgcctt ctccaactac attatctagc cagttcctta
     7021 aaaatctctc tgagtaaggt agtttactta tatatttact aaaaatccta aaccttctat
     7081 cctctaaagt ctcatttgac ttgggattta tagacattat cttttcccat ctttttatgc
     7141 cacttggagt taaatcctct aaaaactggt cacttgatag gtcatttaat ttttcatgta
     7201 gtgtttttat ttctttgttt tctgcatctt cccaactcat atttaactca tttaaatagt
     7261 cgtttattgc ttcttctgct atagtcttta tattttccca agtgtagcca tttttatatg
     7321 ttatctctgc tgatatattt atagttgtac ttgtaattcc ttcaactgtg actctatgac
     7381 ctattggtgc taatccaaga ccttctcctt ggttttgtag agggtcaatt tcttcttgaa
     7441 ctaaattaac taaatcacta gatggaactt tgaagttaga attaattatt actaacttaa
     7501 cagtacctcc accgttccaa actggatata ctttaactcc cccaacgtca agcaatttat
     7561 taacttcatc cttatagttt tgtatattcc ccccaaaaga ttgagaattt aaactatcat
     7621 aatatctttg tcttaaactg tcctcagatt cttcatcttc tccatttatc agtatgtctg
     7681 ttaactcagc agtttcaaga ccatctatat atccaattgg aatcaactga ccaagttcaa
     7741 aaatagttcc agcagtttca catttcatct tatatattcc ttcactaatc ctttcggttg
     7801 caatataatt gtactctcct aagttgaata ttgaatcaat aggaatatct atattaaaaa
     7861 ctccttttgc aattgtattg gttgcaggta aaggtgtaat acctctctct ttacatctct
     7921 tctctaaata ataataactg gcagtatcta cgaatgtttg gtctaacaat tcatccattg
     7981 caatgtatgt ttctgtgagt tctatggcaa caggagcaag agcattatat attatagacc
     8041 cttctctttt atcaaaagtg tctggtacac tatctaacat tcttttaatt atattttcaa
     8101 aagtcattaa ctcaaacact aaatactcac caccttctat tctatattat taatcgtctt
     8161 tttaattata
//
LOCUS       DAEMAE010000166         8057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEMAE010000166 DAEMAE010000000
VERSION     DAEMAE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.168"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(7..606)
                     /locus_tag="KPH93_002824"
     CDS             complement(7..606)
                     /locus_tag="KPH93_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG1689618.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIYGSEKLSFQEDKEFIEAYVDGSYEHSIKMYGSGVVILKNNEVIKTYSEKGKEE
                     SLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKKGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(624..3743)
                     /locus_tag="KPH93_002825"
     CDS             complement(624..3743)
                     /locus_tag="KPH93_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBG1689619.1"
                     /translation="MDNIISVLNDVVFLDIEVTGLDCLNSEILEVGAVKVKDWKVYTY
                     ESLIKNKFEVPIEVFSVCRNLDKNDLEIANEIEMVEDRLVNFVEDNFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSRFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGELLSLIFLK
                     RLVEGGKYGDIEEINYSVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIEREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGVDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYSIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNLSVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            3964..4788
                     /gene="pdaA"
                     /locus_tag="KPH93_002826"
     CDS             3964..4788
                     /gene="pdaA"
                     /locus_tag="KPH93_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG1689620.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPDLIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(4884..5669)
                     /locus_tag="KPH93_002827"
     CDS             complement(4884..5669)
                     /locus_tag="KPH93_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689621.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     FQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGIETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDIAGKIYHNIGYKEVNEWTILFK"
     gene            6134..6499
                     /locus_tag="KPH93_002828"
     CDS             6134..6499
                     /locus_tag="KPH93_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689622.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            6577..7131
                     /locus_tag="KPH93_002829"
     CDS             6577..7131
                     /locus_tag="KPH93_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG1689623.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PIIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            7351..8025
                     /locus_tag="KPH93_002830"
     CDS             7351..8025
                     /locus_tag="KPH93_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG1689624.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT         2794 a         1258 c          823 g         3182 t
ORIGIN      
        1 tttttattat acaccaatag cttttttggc taatttatca gcttcctcat tatacttatt
       61 tccagaatga gctttaacct ttgtaaattt tacattaagc ttattcttaa tactgtcata
      121 gaaattttta tatgctatag ttcctttttt atttgttttc caaactccag tacaccactt
      181 ttcaatccct tcatagtcaa agtataaaat caagttttga acattattgt caatacagta
      241 ctgcattgca attttagaag cttctatctc tccagctaca tttctcatac ttactaaaga
      301 ttcctctttt cctttttcac tataagtttt tataacttca ttatttttta aaatcacaac
      361 acccgaacca tacattttta ttgaatgttc ataacttcca tctacatatg cttctataaa
      421 ctccttatct tcttgaaaac tcaatttttc tgacccataa atatattctt tagcctcttg
      481 aaaagtagaa aaactcttat attcagctcc tgagaatcca tttacttgtt ttttgcactc
      541 atcccatgta ttatatacac ctatattttt accttttttc acagcataaa acttttgttt
      601 tgccaaacat atcactccca ctcttaattt ttatcttctt ctctcataaa tatatctaaa
      661 tttataagat agtttctatc aaaatatttt actttcatat ctttttttat aatatcaata
      721 tggtacactt ctccttttac agctctttgt cttatatttt catttatatc tttttttata
      781 atatccttat caacatcatt gttatccatt ttagcatcaa cattcaacgc ttttatagta
      841 tcaaatataa cagattttag tattaaactt ctagatttat ttaaatgtcg ccttatataa
      901 gtataaaact cattgctatt aacctttgaa attttacaat cgtgtaaatc tttctctaat
      961 ctctttaaca cacttagatt agttcctaca tcaaaaatca caaaacatcc atagtcatat
     1021 ttgcttctta aaattctccc catagcttgc tttaccttta tggtcatttg aggataattt
     1081 atagtatagt agtctatgct atacttcttc attatagtaa aatacaaagg gtcttttgga
     1141 tttagatttg gaagcttgtc taaagttaca caaaccaaac catcccctgg aatgtctacg
     1201 ccttcaaaac atccttttga gccaagtact acacattttc tatttaaatc attaaggtgt
     1261 ctaatgccct ttttatcagc ataaatttct aagttaaaac tatgtaagta ttttttcaat
     1321 atttcataag ttttttcttg tctatcttta ctattaaaaa gagctaatgt atgaccttct
     1381 gtaatctttg atatattaga tataattttg ctcatttcat ttggaaattc agaatttctg
     1441 tactcgcaaa tatcagaaaa tccaactaca gagactctat ttttataatc aaaaattggt
     1501 tctataatct tttccacatt cttaactctg tctattccta atgtattttt aaaataagac
     1561 atactttcag ataaactcaa agttgcagat aaaaatattc ctttttctaa ttgagataaa
     1621 atattttctt caaataaatc tgctattttt aatggcacta ctcgaaactc aaagtcatta
     1681 taattcttat caatttcaac tatccttgca taatcatctt tttcatcata ctccaaaaag
     1741 atttcaaata taatctttat atcctctaaa tctcttgtct ttgcctttcc aaatttatat
     1801 atatctgctt ctttatctat actatcatca tcaatattcc tataaattat tattattatt
     1861 gaaactaaat tttttattat tttctcacaa ctcaacttta tcttttcact ataagctcta
     1921 taacttattt cagtatctac accatccttt tttagttttc ccacgatttc atctatttgc
     1981 aagttcagtt cccatcttaa attataatta cttacattat tatattcatt acaattacca
     2041 aaatttaata ttgaatctat ctcttcaata atgagattta tgcttctagc aattttttgt
     2101 ttatcttctc tttcaatttt taatacatta taaaatttat caaaagcctt tatttttctc
     2161 atatttctac tatatttttt atatataaag ctactatttt gaataaactc gtaaggataa
     2221 atttcttgta ataaatacct taaagacttt gaatttataa tgcttgaaaa gaaatcatat
     2281 cctttttcag tcaaattatg tgcttcatct actattatat tttctaaagg tttttcatcc
     2341 ttatacggcc atttagctaa tagtgaatga tttactactg ttatatgttc ttctttcaat
     2401 tcttctattc tgtttttata taaacaatct tttttgcatt ttttaggtct gcacatattt
     2461 gggtcacaac ttacatttct taagtgagta cttatttcct tgaagttgtc aaacaccgaa
     2521 taatttattt cttctatatc tccatacttg ccaccttcaa ctaacctctt caaaaatatt
     2581 aaagataaaa gttctccttt agtagggttt tgagactcat aatcatctat atatgcttcc
     2641 agtctatcta tacaaatata gttatttttt ccctttatat atccgtaact tacttttcca
     2701 tttaaaccca aagaatctaa cacatttgga atatctttat ttattaattg tatctgcaat
     2761 tcctttgtat cagtagaaat taataatctt tttttattaa ttctagcctc taatattgca
     2821 ggcaataaat atcctacact tttacctata ccagttggtg cctctataca tgcaattttt
     2881 tcttcatctt cgctatttct aaacaactct cttattgttt tagtaagctc atattgacca
     2941 ggtctatact catatgtaaa tccttcttta ttctcccaaa tagttttgta ttttaataat
     3001 tcttcataat ttttttcttc ttcatttaaa atttttaagg tttctctttc cttcttcttt
     3061 tcttcttttg agtcaaatat attatattct tttttgatat ttatattatt tgataaatca
     3121 taatttgcct cttctaaaaa tctgctccat tcccatgttg gaagattaaa ttttttaaga
     3181 tatgaattta ttttaaaact caatggctcc aaagtagttt tttcatagtt attaaatttt
     3241 actaaaaggc aatttacaat atttattata tcaatagcat ctgataaagc tctattttcc
     3301 actttagaat tacaatttgt taaagtattt tttaagtatt ctaagctata atctttgtga
     3361 tatggttcta aaataactgc taattcaatt aaatcaataa atttattttt taactttggc
     3421 atatgatact caaaaaactt ttttttcaga cttaaatcat gacatataat gaagttatct
     3481 tctacaaaat ttacaagtct atcttctacc atttctattt cattagctat ttctaaatca
     3541 tttttatcta aatttctaca tactgagaat acttctatag gtacctcaaa tttgtttttt
     3601 attaatgatt catatgtata tactttccaa tctttaacct tgacagctcc aacttctaaa
     3661 atttctgaat ttaaacaatc taatccagta acttctatat ctaaaaaaac aacatcattc
     3721 aatacactta ttatattatc catttttata tcccttcaag ttaaatcagc ctaacttatt
     3781 tacaatcatt tctctttata ttttatcatt tatatacact ttatttaaga taattaatat
     3841 caactttata tcttacagta aacttctttc atttattatt tgtatatgtg aatattttta
     3901 actataaata ttcaaactat attataagaa atatacttta aattttaaat aagaggtgaa
     3961 attgtgaaaa aagatagcct aaaaaaatat attatgatag gagcatttgc tctaatactt
     4021 tttggaattg ccagtataaa ttttaaatca ttagataaaa caaaaactca aatttctagt
     4081 ccaacactcg acacacatga atatgattgg tattttaacc ctagggagga tggtaaacag
     4141 ccatcaccta taaaagaagc cgattttttt aaaaaatacg gagcttatta tgtaggaaat
     4201 cccaatgaaa aagttatata tctatcattt gatgctggat atgaaagtgg aaacactcca
     4261 aaattattag atacgcttaa aaaacacaat gctaaagcgc aatttttcgt agttgaaagt
     4321 tatataaaaa gcaatccaga tttaataaag cgtatggaaa aagaaggaca tttagtttgt
     4381 aatcattcaa aaagccatcc atcaatggca ggtattacag attttgaaaa attcaaagaa
     4441 gaaattacaa gtgtagaaaa agcatacaaa gatgttactg gaaaagaaat gcctaagtat
     4501 tttagacctc caatgggtaa atttagcgaa caatctttaa aatatacaca agatttagga
     4561 tataaatcaa tattttggag ttttgcttat gtagattggt atgaaaagaa acaacctact
     4621 catgaatttg ccaaaaacaa aatttattca cgaactcatc ctggtgcaat agttttactg
     4681 catccaaatt catcaacaaa tactgagata ttagatgagg ttcttacaca ttgggaaaaa
     4741 gaaggttata aacttaaaac acttgattat ttaaataata aaaaatagta tcataaatta
     4801 aaaaaactct atcatgaatt atagtaaaaa tatagttatt tataaatact ttaattctcg
     4861 atagagtttt ttatttttta tagttattta aagagaattg tccattcatt tacctcttta
     4921 tatcctatat tatgatatat tttacctgct atatcattgt tataaaaaag acatggaact
     4981 tttccttcca acaataattc tttactcaaa ttgtaaacca tataacttgc aagtcctttt
     5041 cccctatatc ttttatctgt acttacagag accaccattg ccaaaaaact agtttctata
     5101 ccagttgaca cagtgctaat catagtatca tcatttctaa taaaatatgc tcttacattt
     5161 ccctctttta aatgttcttt cctttcttct atgtaatttt tacttacttt atagtctgac
     5221 ctatttaaaa gttttcctat ttcatcaata tctttttcct gagcattttc tattttatac
     5281 tccttcatgt tggaaaaatc tatttctttt atttctttta atacacaaaa tttattatct
     5341 agtttttcat aaaaatcttt gtatttactt attagtctat ctattacaca tttttttcca
     5401 cttatgcttt ctacatctaa atcttttata tgattcttca tttctcctat atcaaaatta
     5461 tttttaaaac tataaattat aagtgttgac ttatttttta atattataga ggttatattt
     5521 ccatcttcca cctgaaacca tatatcttga tattgagagc taaatccata attctcaacg
     5581 tatccaatta tgaataaatt taaactttct tcatgtgata catattgcca aaattctttt
     5641 tccaaagtat tatctatttt tacaatcaag catatatcct ccttctatgt taataatgat
     5701 ataccttata atttatagta tcatttaaaa ccattatatt ctaccaatta ttctaaattt
     5761 ttaaatatct atgcaatatg ttcgtattta tttttatatt ctgttactgc ttcactccaa
     5821 gcttcttcta ctgaaatacc tctcttattt gcaatttttt gtgctaattc tattattttc
     5881 atagcttttc ttgatatatt catatatatt cctcctctaa attatcataa gttattttaa
     5941 agttaaattt ctatagtcat gttatatatt tatttactta tatcttacta attatttatg
     6001 tactaaatat gaactacaat agaagaaatt tagaacttaa taaaacataa aatttatata
     6061 cagttcatat atatttgtca tatcacaaga caagtgatat aatttattat cattttactg
     6121 ggggtgatat gctatgaaaa taagagctga atatacatgt cctcttgaaa ttgtacatga
     6181 cataatcaaa ggaaaatgga aaactatcct tatatttgaa ttaagacaat ctaataaaac
     6241 attttctgaa ttgcaacata atatttatgg aataaatcaa aaaatgttga ttcaacaatt
     6301 aaaagagtta cgtgattttg ggattattga taaaataagt tctgctggat atccattaca
     6361 tgttgaatat tttcttacta atcgtggtaa aaaaatgtta aaagcagtgg aaataatgca
     6421 agaaatagga atcgaatata tgcttgaaca tggtcaacaa gaaattctag ataataaagg
     6481 aatttgctac aataagtaat cctatacata ccaaaaagta agtaatttac attttttaca
     6541 atagtattat aataaaaata aataggaggt ataatcatgt ctatagcttt aatattgatt
     6601 gatattcaaa atgattattt taaaaatgga aaatgtgaat tatttcaatc agaagaaact
     6661 gctgaaaatg ctaaaaaaat actattgttt tttagaaaaa acaatttacc aattattcat
     6721 atacagcata tatcaacaga taaaacagct tctttttttc ttcctaatac tcatggttca
     6781 gaaataaata aaattgtttt tccactagat aatgaagaaa ttataactaa acacacacca
     6841 gatagtttct ttgaaacaga tttgcaaagt atccttgaga aaaaaaatat tactaatcta
     6901 gtaatctgtg gaatgatgag ccatatgtgt attgatacaa gtgtaagaac agcaaagaaa
     6961 cttcgatacg acataactct aataagcgat gcatgtacaa cgaagaattt atcttggaat
     7021 aatatagaaa ttaatgcaaa tactgtacat caagtattca tggcatcact acagaacagt
     7081 tttgcagatg taaaaaacac agataaattt ttatctaact attacaaata aaaacataca
     7141 ctatgttaaa agagcaagtt tgatttattt aagcctgctc tttaatcatt taaaattaga
     7201 tgctttctat catttttata tacataatat acgtttatac attttatatt catatataac
     7261 tattatataa attttaagct ttactatatt cacataaaaa atgtagtatt atagtatata
     7321 taatattata tagaaggggg tgctagataa atgaaccatc atatattgat tcaatttgaa
     7381 tatatagcaa gaatcattac tgctggtttc tgtggggctt taattggata tgagagaaag
     7441 aatagactaa aagaagctgg aatacgcact cattttatag tggcattagc ttcagcactt
     7501 ataatcattg tttccaagta tggatttaat gatatagtgg gtactccagg tattggtcta
     7561 gacccagccc gcgttgcagc acaagttgtc agtgggatag gttttttagg tgctggatta
     7621 atatttatta gaaatcaatc agtaagtggt cttactacag ctgccggtat atgggcaaca
     7681 gccggtacag gtcttgccat tggctctgga ttatacttag ttggtattgt ttcaagtatc
     7741 attattgtta ttgtacagat aacacttcat agagatagga aatggatgaa actccctaca
     7801 gcagagcaag tttctcttaa aatttctaac agcactgatt ccatagaata tattgaaggt
     7861 aaatttaaaa aatctaatat tgaaattata aacatgaata ttaaacgtct tgacggggat
     7921 tggttagaag tagttatata tactaagtta cctaaaggat ataaaaaaac aaatttatta
     7981 aaattattta gcgataatcc tcttatagaa actttagaaa tataatataa aaattgaagc
     8041 ttaaaaataa taatata
//
LOCUS       DAEMAE010000167         7993 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEMAE010000167 DAEMAE010000000
VERSION     DAEMAE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.169"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..455
                     /locus_tag="KPH93_002831"
     CDS             <1..455
                     /locus_tag="KPH93_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1689625.1"
                     /translation="LVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYILDQII
                     NNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGIDEEDISRVFDKGFTGKNGRNQLY
                     KSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDYLSLDS"
     gene            886..2046
                     /locus_tag="KPH93_002832"
     CDS             886..2046
                     /locus_tag="KPH93_002832"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG1689626.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKNEIRKCCIEEESSVDVICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITMIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYSYKNFILPKVNVDTLLIGGGGAHNLTLIRYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            2048..2437
                     /locus_tag="KPH93_002833"
     CDS             2048..2437
                     /locus_tag="KPH93_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689627.1"
                     /translation="MSNNKKKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFEIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            2701..3012
                     /locus_tag="KPH93_002834"
     CDS             2701..3012
                     /locus_tag="KPH93_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1689628.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEAGVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     Y"
     gene            3098..4444
                     /locus_tag="KPH93_002835"
     CDS             3098..4444
                     /locus_tag="KPH93_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1689629.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKANAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIVIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            4677..5774
                     /locus_tag="KPH93_002836"
     CDS             4677..5774
                     /locus_tag="KPH93_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG1689630.1"
                     /translation="MGRLGISIYPEKTTFKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWSVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DNLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGCIRKEELFILDYIKPWQKFNFKPSTK"
     gene            5873..6787
                     /gene="murQ"
                     /locus_tag="KPH93_002837"
     CDS             5873..6787
                     /gene="murQ"
                     /locus_tag="KPH93_002837"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG1689631.1"
                     /translation="MLIKTLENLVTEDRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVDIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAVEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNIKYDIAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKNILDRQEGYISEALKSIETL"
     gene            6897..7592
                     /locus_tag="KPH93_002838"
     CDS             6897..7592
                     /locus_tag="KPH93_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG1689632.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFD"
BASE COUNT         3159 a          842 c         1479 g         2513 t
ORIGIN      
        1 aattggtaaa aatgtctata aaaaataatt catttttgct tataaaaaac aatgtagaag
       61 ttagtttgaa tgaattagaa aatgatgttt atacagattc taaatgtatg tcatatattt
      121 tagaccaaat tattaataat gctataaagt attcaaagga agttggtaag attgaattta
      181 attcaaaaaa gttagaaaat ggagtggtat tatctataaa agattttggt ataggtatag
      241 atgaggaaga tatatctaga gtttttgaca agggatttac ggggaaaaat ggaagaaatc
      301 aattgtataa gtctactgga atggggatgt attttgtaaa gaaaatgata gatagtttag
      361 ggcatgagat tgaggtatgt tcggaaaatg gttcatatac aatttttaat atctattttt
      421 atgatatatc ggattattta agcctagata gttaggttat tggactataa aatctatcac
      481 catatacaaa tcgacatata caataagaat aagtttagtt cttgaaaata gtgaagattt
      541 aattagtgcc tcaaatgatg ctctatctat attataaaat aataattatt atatatgaaa
      601 aacatatcag atagatacct ggtatgtttt ttatttgtaa taactatttt aatatggttt
      661 ttggagaaat aatatgcttt atttatgaaa aaaaattaca tgatattaca ttgacactga
      721 aaatgaatta aggtaagatt tagctaaggt aaaaaacgtg tataacatta actcaattaa
      781 ttttgtaaga gtaaattatc ataattaaag atgaaataag attaattcta aaattattaa
      841 acttaatatc ttgatttggg gaggaataac tataaaatta aaaggatgat aactatgtat
      901 agtgtaggtt taatgtcagg aacttcctta gatggagttg atgcagtact agctgaaatt
      961 agtgggaatg gtagaaatac taaagttaag caaatagaat ttataactct agaaattcct
     1021 aaagatataa aaaacgaaat aagaaagtgc tgtatagaag aggaatcgag tgttgatgta
     1081 atctgtagct taaattttaa gcttggatat ttatttagta aagctgtaaa atcagtgtgt
     1141 cataaagcaa actttcatat agcaaatttg gattttatcg catctcatgg tcaaacaatc
     1201 tttcatatac caagaagtta taataatttt gtatcttcaa cgcttcaaat aggtgaacca
     1261 gcagttatag catatgaaac aaatacaaag gtaatatcta attttagagt aatggatata
     1321 gcagctggtg gagaaggtgc tccattagta ccatatagtg aatttttatt atatagtgat
     1381 aacaataaaa atttagcact tcaaaatatt ggtggaatag gaaatattac tatgatacct
     1441 aaatcatgta acatagatga tgtatttgca tttgatacag gaccaggaaa tatgataata
     1501 gatggagtat gtcaaagatt atttaataga aagtatgata aaaatggata ctttgcatca
     1561 aaaggtaaaa ttaatgaaga aatgttaaaa gatttgatga gtcataagta tcttagtcaa
     1621 gcgccaccta aaagcactgg aagagaggta tttggacaag tctatttgga taacatgctt
     1681 aacaaatata aacatgtaga taaatatgat ttgatagcaa cggtaactat gtttacggca
     1741 aaagctatat attatagtta taaaaacttc atacttccaa aggttaatgt agatacttta
     1801 ttaattggag gtggaggagc tcataatcta actttaataa gatatataaa agagttatta
     1861 ttagaggttg aagtattaac tcaagatgaa tatgggtatt cttctgatgc aaaggaggct
     1921 ttggcatttg taatcttggg caatgaaaca ttaaataatt ctttttctaa tgtaatatct
     1981 gcaacagggg ctaaaaataa agttatttta gggaatataa cacctaaacc ttttggaggt
     2041 aaatgacatg tctaataata aaaaaaagaa aaataataat tataaaaatg caaaaagtac
     2101 aaaaagtaat aaaaatacta agaaacaaat ggagaaaaat caaagaacag attataatat
     2161 tccaactata agttttaaaa gtggattaat attaatagtt tcatctctag taagtactgt
     2221 agtttttcct ttgttattat ctatatttga aatagataat agatttggag ttgttatagg
     2281 aaattcgtta atcacatctt ttgcagttgc atatacaaga tactttgttg aaactaagag
     2341 aggtttttct ttaggatttt ttagacttta tattttattt gctttatcat ttgcaataat
     2401 aggttacttc tggatatata aaggaataca tatttaaaaa agtatggaga ctattttatg
     2461 ttaataataa tatagtcttt tttctttttt gtaagaaaag atatgagtat tttaagtagt
     2521 gtgtaacttt agcaaaaaga atgactaggt aaataaagca attaaagcaa gatataaata
     2581 tatgaaataa aattacaatt aaaattgtaa attatgaaaa aatatataga aacgtattcc
     2641 tgtaatcaaa aatacatgtt ataatgattg aaaagaaata aaaaaacagg gggaatcaat
     2701 atgagagtat tatttgtttg ttcatcaggg atgtcaagtg ctatagcagt taatgcatta
     2761 caaaaagaag gagcaaaaaa tggaatagat atagatgttt tggctgttgg tactcaagag
     2821 tttgaggatg aagttaaaaa tggatgggat attgctatgg tagctccaca agtcagacat
     2881 agatttgact atttaaaagc ttttgctgat gaggctggag taccatgtgc attaatacaa
     2941 gcgcaagctt atagtccatt aggaggacca aagctattaa aacaagttca agagctgttg
     3001 agtaaatatt agttgcagta ttgttaggtt agaagaaagt taattttata aaaattttaa
     3061 tgataataaa aataaatatt aattaaggag agaaaagatg ctagataaat ttacagaatt
     3121 tttagacact aaattatcaa cttctatggc cagattggca gaacaacgtc acttaagagc
     3181 tgtaagagat ggaatcgtag cttcattacc acttataata gttgggtcat tttttttgat
     3241 attagcattt cctccactac cagattcatg gggagtgact cagtatttaa aggcaaatgc
     3301 agttacaata ttactaccat atagaatgac attttttatt atatcagtat attctacatt
     3361 tggcataggt tatagtttgg caaagtcata taagttagat ggattgacag gaggtttatt
     3421 agctacagct acattccttt tgacaatagt tcctactgca atagagggtc ttacccctga
     3481 tttattaaag atagttgaaa agagtccaga acttttagct tggtataatt caattcctca
     3541 aggatggcaa atgccaatgg ctaatcttgg tggaggttca atgtttgttg ctattgtaac
     3601 atctataata ggtgttgaga tttatagatt tactgacaaa tctaagttca agataaccat
     3661 gccagaagca gttccagcat cagtcacaag gtcttttgaa gctctgacac cagccgcatt
     3721 aataataatt ttaatggcta ttgttactta tttcctaggg tttgattggc atggcttcat
     3781 agctaaatta gtaactccat tagtttcagc cactgacaca ttaccatcag taattatact
     3841 aatattttta ataacgtttt tttggtcatt tggaatacac ggtgtatcga taataggctc
     3901 attggcaaga cctttatgga tacaattatt agatgctaat acagcttcat atgctgcagg
     3961 tcaagcagtt ccaggtattg ctgctgaacc attttttcaa tggtttatat ggattggagg
     4021 ttctggagct acaataggtt tagctttact tcttgcattt agagcaaaat cagcttatgg
     4081 aaaatcttta ggaaaagcaa ctgtaatacc agcaatattc aatataaatg aaccaataat
     4141 atttggagca ccaatagtat taaacccaat attgataata ccatttgttg tagcaccaat
     4201 agtaatggca acagtttcgt ggtttgctat gagtgctaat ttagtatcta gagtagtagt
     4261 tactgctcct tggacattac caggaccagt aggggcatat ttagccactg gtggggattg
     4321 gagagcttca gttcttaact tattatgtat agctatatca atagtaattt actatccatt
     4381 tgtcaagatg tacgataaga ggttacttca tgaagaaaaa gttgaagagc aagaagctta
     4441 ttaattagaa actatattgg aaattaaaaa ctatattaga ttagtataaa tttcaaagta
     4501 taacagttat gaaataagta acgaagatat tttgttgatt gtttataaaa aaagaaaagg
     4561 taaacttttc tttttttata aatatatatg aaactttttt tcaccaaatt tttaaaaaag
     4621 tttcgtgtaa ttcaaaataa atttaaacac atatagggta taaggagatg acacagatgg
     4681 gaagattagg aatatcaatt tatccagaaa aaacaacatt taaggaaata tatgattata
     4741 tagataaagc tgctgaaaat ggatttacac gcatattttc atgtttatta tcagttaaag
     4801 atactaaaga aaatataatt aaaaaattta aaagtataaa tgaatacgct aagtttaaag
     4861 gttttgaggt aatacttgat gtaaatccca gagtatttgg tgaattagaa ataagctaca
     4921 aagatttgac tttttttaaa aagacgaagg cagacggaat aaggcttgat atgggattta
     4981 caggttctga agaggcatta atgactttta atcctcaaaa tcttaaaata gaaatcaata
     5041 tgagtaataa tactaattat atagatacaa taatggacta ccaaccaaat aaagaaaatt
     5101 tgataggatg tcataatttt tatccacata gatatagtgg attaaattta gaacacttta
     5161 taaaatgtac agaaagattt aataagtatg gattaagaac agcagtcttt gtaacaagtc
     5221 aaaatgaaac cacatttgga ccatggtctg taactgatgg gcttccaaca attgagatgc
     5281 acagaaatct acctatagat attcaagtaa agcatataat agctatgaat aatataaatg
     5341 atataataat atcaaattgt tatccaacat atgaggagct aaaggtatta ggttctatga
     5401 gaaaagatat ggttacattt gatgtagcaa tagaggaaaa tatacgagaa gttgagaaga
     5461 agatactctt tgaagaaata cattttaata gaggggacat atctgacaat ctgataaggt
     5521 ctactcaatc tcgtgttaaa tataaaggat acaaattcga actatttaat actcctaaaa
     5581 taataaaaag aggagatata gtaattgaaa gtagtgaata tggtcattat gcaggggagt
     5641 tacaaatagc tttgactgat atgaaaaaca gtggtaaatc gaatgtagta ggatgtataa
     5701 gaaaagaaga attatttata ttagattata ttaaaccatg gcaaaagttt aattttaagc
     5761 cttcaacaaa ataatttatt atgaaaatat taatttatta tgtagatata attatgaagg
     5821 ttggaattaa aatatgttta aaaatttaat attaagaaaa tgaggaaatg atatgttaat
     5881 aaaaactttg gaaaacttag ttacagaaga tagaaataag aatacattgc aaatagataa
     5941 agaagataca ctaggtataa tagaattaat aaataacgaa gataaaactg tagcctatgc
     6001 tgttgaagaa caaaaggaga gtatagcaaa agctgttgat ataatagtag atagaatgaa
     6061 acaaggaggt agattattct atataggagc aggtactagt gggagaatag ggatactaga
     6121 tgccacagaa tgtcctccaa cttatggagt ggattttgag cttgttcaag ctataatagc
     6181 tggtggaaat caagcaatat ttaaagcagt agaaggtgct gaagatgata aagaacttgg
     6241 aaagcaagat ataatagata gaggagttac atctaaggat gttatatgtg gtatagctgc
     6301 atcaggaaga actccttatg ttataggtgc aatggagtat gctaaggaat tggggtgtgc
     6361 agtactatca ataactatga atccgaatag tgaaatgagt aaaaaagcag atttaccaat
     6421 aaacattata gtaggggcag aagtaataat gggttcaact agaatgaaaa gtggcacagc
     6481 tcaaaaaatg gtatgtaata tgttaacaac agcttctatg gttaagatgg ggaaagtata
     6541 ttcaaactta atggtagatg ttaagacatc aaatgaaaag ttggttgaaa gagcaaaaag
     6601 aataataatg atagctacaa atataaaata tgacattgca gaaaaattct tagaagaagc
     6661 agataatagc gttaaattgg ctatatttat gataaaaagt ggtttagata aggatagtgc
     6721 aaaaaatata ctagatagac aagaaggtta tataagtgaa gctttaaaat caatagaaac
     6781 attgtaaaat aaataataat actgataacc tttcaatatt aaaagaacag cattggaagg
     6841 ttatttttag atattaagta ttgaaagaat tattaaaggt attgtaggag gatagtatgt
     6901 attttttagg ggtagatggt ggaggaacga aaacaacatt tacattagta gatgaagaat
     6961 taaatatagt aggaaccata accaaaggaa cttgtcatta taatcaaata ggatttgata
     7021 atctaacgaa attattaata actgggttgg aagaagtttg taaagatgct aagataaatg
     7081 tagaagaaat aacgtatgca tttgttggac ttgctggata tggaaaaata aaagaagtat
     7141 tgtatgcatt agaagtagca acaaaaaatg catatagcca tataaattat actctaggca
     7201 atgatgtaga aatagcatta gcaggttctt taaatgggga aaaaggtata aatataattg
     7261 caggtactgg ttcaatagca caggctttag ataaagatgg gaatcttcac agatgtggag
     7321 gatggggcta tgtattagga gatgaaggtt cagcgtatta tataggaatg gccaccctaa
     7381 aaatgttcac aatgcagtct gatggaaggt gttcaaaaac aaaattatat gatttaataa
     7441 aaagacatct taatatagag aatgattatg acataataaa atatgtaaat gatgaaatac
     7501 aaggagatag aatagaaata gcaaagtttg ctacgatatg tttaaaagca gttattgaag
     7561 gtgataatac tgctagtaaa atatttgatt aaaaaatagc tacgcacact tgtgacttta
     7621 gtcatgagtt agtagctaaa atagtcagca tatagggaaa cctatatgta gagataggat
     7681 agaacctatc caatactact tgaattgcta aaaaccccta aagctaacca aactaaaaca
     7741 taaggatgaa ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt
     7801 ataaggttaa accctaagta ctttaaaatg ggcaatcagc aggtaagctc tgaataggag
     7861 aaacttcaac gactattcct cttgagggaa gtacactaca agctattggt agtggaagtg
     7921 ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttac atgaaagtgt
     7981 aagaagttca taa
//
LOCUS       DAEMAE010000168         7914 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEMAE010000168 DAEMAE010000000
VERSION     DAEMAE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.170"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..1849
                     /locus_tag="KPH93_002839"
     CDS             56..1849
                     /locus_tag="KPH93_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1689633.1"
                     /translation="MNIKDRLSGLRKLMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWQDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLVDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKVKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1930..3312)
                     /locus_tag="KPH93_002840"
     CDS             complement(1930..3312)
                     /locus_tag="KPH93_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG1689634.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSEMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGSSISMYGYRYVFLFSSITVLIGLFLFIFGNRKFYKPLN
                     NYPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDSNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLV
                     MNKKLVELSN"
     gene            3570..3917
                     /locus_tag="KPH93_002841"
     CDS             3570..3917
                     /locus_tag="KPH93_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1689635.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIVQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EENLEFVALVLYM"
     gene            3935..4969
                     /locus_tag="KPH93_002842"
     CDS             3935..4969
                     /locus_tag="KPH93_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689636.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARIKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5018..6067)
                     /locus_tag="KPH93_002843"
     CDS             complement(5018..6067)
                     /locus_tag="KPH93_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1689637.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKVDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6342..7877
                     /locus_tag="KPH93_002844"
     CDS             6342..7877
                     /locus_tag="KPH93_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689638.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKDPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINENDLGKSNKVYATIEVDMKGNQNVDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRENNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
BASE COUNT         3087 a          970 c         1356 g         2501 t
ORIGIN      
        1 ctttacatac ttatatattt agttattcaa actattattt caaggaggtc ttgttgtgaa
       61 tataaaagat agattaagtg gattaagaaa acttatggaa gaaaaaaata tagatgccta
      121 tatgattcca tcttctgata atcaccaaag tgaatatgta ggagattact tcaaatcaag
      181 agaatttata tcagggttta atggttcagc ggggacagtt attgtgacta aagacgaagc
      241 tgggttatgg acagatggaa gatattttat acaggcagag tctcaattag aaggaagtac
      301 aataaaatta ttcaaaatgg gacaagaagg ttgcccaact acagatgaat atttatacaa
      361 aaatattcct gaaggaggaa ctttaggatt tgatggtaga gttatatctg ctagagaagg
      421 agctacatta gctgaaaaat tatctaaaaa aggtataaag atagaatatc agtatgattt
      481 aatagatagt atatggcaag atagacctgc tttatcagat agtaaagcat ttttattaga
      541 tgttaaatac tgtggagaaa gttttagctc aaaattagct agattaagag aaaaaatgag
      601 tgaaaaaggt acttctactc atgtaataac tactcttgat gatatagcat ggttattcaa
      661 tataagaggt ggagatgtta aatataatcc agttgtactt tcttatgcag taataacttt
      721 aaaagaagtt tatctatttg ttgatgaaag caagttaaat gaagaaatac taaatgaact
      781 agctaaggaa aatgttcaaa taaagccata taatgatgtg tatgaatttg taaaaaatat
      841 agacaaaact gaaaaagttt tagtagatgg aactaagctt agttatacaa tttacaataa
      901 tataccttgt gaagttgaga aagttgatga gttcaatcca gtaatgtttt ttaaagctca
      961 aaaaaatgag gtagaattag aaaatataag aaattcacat gtaaaagatg gtgtagcttt
     1021 tactaaattt atgtactggt taaagaaaaa tgtaggtaag atggaaatca ctgaaataag
     1081 tgctactcaa aaattagagg acttaagaag agagcaagaa ggtttctttg agccaagttt
     1141 caatacaatt gctgcatata aagagcatgc tgcaatgatg cactatagtg caactccaga
     1201 gtcaaattat aaattagagg cagaaggatt atttttagta gattcaggtg gacaatacta
     1261 tgatggaact actgatataa ctagaactac agttttagga cctataagtg atgaattaaa
     1321 actacacttt acttctgtag caagaggaat gataaatctt tctaaagtta agttcttgca
     1381 tggttgtaga ggatataact tagacatact atctagaagt tgcatgtgga atatgggaat
     1441 tgattatcaa tgtggaactg gacatggtat aggatttgtg ttaaacgttc acgaagctcc
     1501 aaatggattt agatggagag tagtacctga aagatttgat agtgcagttc ttgaagaagg
     1561 aatggtaact actaatgaac caggtatata tatagaaggt tctcatggta taagaacaga
     1621 gaatgaaatt gtagttagaa aagctgaaaa gaatttctat ggtcaattta tggaatttga
     1681 agttgttaca ttggctccta ttgacttaga tggtatagta ccagaattaa tgaataaaga
     1741 tgaaaaagat tatttaaact ggtatcataa gttagtttat gataaaatat cacctttctt
     1801 aactgatgaa gaaagagaat ggttaaaagt ttacactaga gctatatagt ttgtactaga
     1861 tacaataaaa atcgaggtat tataatgcct cgatttttta ttgtatattt tattaacttt
     1921 aagaaatttt caatttgata attctactaa ttttttattc attacaagta gtataattcc
     1981 aaatatcaat aaaaatgatg gtatcactat gaagacttgt aacataccaa gttggtctat
     2041 aatagcttgc gtatagccag ataatttatt tgctgcaaat ttgcctaaaa accaaacacc
     2101 cattaggaat gaaatatact tttcaggcga aagtttattt accataccat acccgactgg
     2161 ggaaaagcaa atttctccta tactttggaa tgttaaaaat ccaactaccc aaaatacact
     2221 tgctttgtga attgaattag agtctgttcc tgtttgtata actgctacta tcataaataa
     2281 aaaagctata gctaatagta taaagcctaa gctcattttt tttggcacac ttaaatcacc
     2341 ttttttagtt tgagataatt ttacccatac taaagcagag ataggtccaa gaataacaca
     2401 aaggagacca ttatatgaat ctatccatga agtaggaatt tgaaaattgc caacagtaag
     2461 atttatgtgt tcctttgtat agagtgcaat agacatagag gcttggttat atgctatcca
     2521 aaagaatact gtgaaaaaag ctaacaccaa tattactatt attctttttt tctcaatcaa
     2581 agttaaaggt cttgaagcga gcattttctt atattctagt gattttctta tagactctct
     2641 cccattacta gggtaattat tcaaaggttt ataaaacttt ctgtttccaa atataaagag
     2701 gaataaacct attagaaccg taattgatga aaataagaat acatatctat atccatacat
     2761 acttatagag gatccgcttt tcatagcaaa ccattcatta gcaaccaaac cgcatattaa
     2821 agggccaaat aaagtaccta gattagtagc catgtacgta atagagaaag ctccatcttt
     2881 tcttgggtca tcattggtat ataagtttcc aaccatagta tgtaagtttc ctttataaaa
     2941 acctattcct aatccagata aaaatatcat gatgtttata tccatctcac tatttgctat
     3001 atatcctaga aaataaccaa ttgaaaccat tagcattcca agtataatac agtttttggg
     3061 acctaaaaat ctatcactga cccatccacc gataactgga gagaaataag tccatgctaa
     3121 aaaagatgca agcattgatg cggattctgc tggtgttaac cctaaaccac ctttggagac
     3181 ttgttcagct aaaaacaata ataataccaa tttcatacca tagtatgcaa gtcctacaaa
     3241 ggtaaatatt gaacagcaga aatataaatc tctaggttgt ttaagttttt cttgcgtttt
     3301 taaaactgac atatgttgag aacctccaga taaatagttt aacaaaattt taccatacta
     3361 tatgagtata tgtttttttc atatagacgt caacttatga gataatttaa ttaagtcgaa
     3421 atatatgtaa aaatagtatc atttttgtgt attcagtaat atttttttat gataatatat
     3481 gttattatta tattaaaata caaacatatt tatggaaagg atatgccaat ttagtagtta
     3541 gattgattgt ttggcaggtg ggatgagcta tgataagaaa gaaatctgaa ttacaagtag
     3601 aagtaaaaga aaatttaaat ggtggtatag gaaaggttaa gcttgaaaac atagtacaaa
     3661 atgaagagct taaaggaaaa ggaagactat ttaaaagagt agctttacca gtaggaagtt
     3721 caattggtgt gcatgaccat acaacagatt ttgaagtata ttatatacta aagggaaaag
     3781 gtaaagtatt tgataatggg gaatttgtag aagtaaatga gggtgatgta gtgtatacag
     3841 ctgatggcga aaaacattct atagaaaata taggagaaga aaatcttgag tttgtagcac
     3901 tagttttata tatgtaaaat atggaggagg agtcatgaca ggcaagatag aagaaattaa
     3961 ggacaagata aatagatttt gcaactccaa tttaaatcaa gaatacaaag atataagctt
     4021 taaaattttg caaaaccttt tagataataa taaagagata attcattctt ctagagctga
     4081 tatatggtct tcagctatac taaatatagt tttagaacaa aatcttttat ataataagaa
     4141 gcatccacta catataacaa aaaaagagtt ttcaaaacaa ataggtgttt ctttaaatac
     4201 tataaataat aaatcgtctt taataaagga tgtttgtagt attgactttt taaatcaaga
     4261 tactatagct atttctaatg tagaattttg ggtaaaagag accaattcca aatcaaagtc
     4321 tgtagattta gatttagaaa agaagaaaat actatataaa agatatatta agctatcaca
     4381 agatgcgaat agtgatattg aatcaattag atatatggag gaagctgtaa atataggtaa
     4441 aagtatgatt actaaagaag ataaagaact tggattttgg aaaggtttat ctaccagagc
     4501 ctatatgata gcacttgaat cccttgctag aaagttagaa agtattaata atctaaagga
     4561 gtccaaaaaa gtattagaat atttaataga aattaatcct agtgatgagc agggaataag
     4621 atacaaatta ttaaatgtat taattagatt aaatgataga acttcaataa ataaattatt
     4681 tgaactttat aaagaagaaa aatctgctac atggatgtac agtaaagctc tttattactt
     4741 taaaaataaa agtatgtttt tggcaaatga ttctattaag gttgctagaa ttaaaaataa
     4801 gtatgttgga ttatatttgt tagattggcg aaatgctttt ggtagagaat ttgttacaga
     4861 ggaagaaaag gcagaagctg tctattacta tgatgaaaac atagtgattt ggaatgaagt
     4921 taaaggttct atggattggc tattaaaaaa aatgttggaa ttttcataaa aaaaactact
     4981 ccttttactt atataaaagt aaggagtagt ttttaattta cacatctagt tttaaatctt
     5041 caggttttat tagacctttt tttctcataa attctgaaat caacaaagtt acaatagcag
     5101 gaagtataaa attaagtaaa agtataccaa cccacataga tatacctccc tttccagcac
     5161 tttgcatagc agttactgtt ccaaattgtc caaccaatcc acaagtaccc ataccagaac
     5221 caataggaat attttccatt ttaaatattg ttgtagataa tggtccaagt aatattgaag
     5281 ctacagtagg tggtatccaa attttccaat tttttattat attaggtact tgcaacatag
     5341 atgtcccaag accttgtgcc acaaggccac caactccatt ttctctaaag ctcataacag
     5401 caaagcctac catttggcaa caacaaccta cagttgctgc cccaccagca agaccaccta
     5461 aacttaacat catacaaata gctgcactac ttataggtaa tgtaagagct attccaacaa
     5521 gtgcagaaac taaagctccc attattatag gttgcatagt tgtagcttgc attattatat
     5581 ttccaaatga agtcataaaa gcagaaacag ctggaccaat aagctcagct acaagtactc
     5641 caattagtat tgtcacagca ggtgtaacca atatatctac ttttgtttct tttgaaacta
     5701 attttccaac ctctacagca aaaagtgtag cgacaaatgc tccaacaggt cctcctaatt
     5761 cattaccaca agctccaaca agtgcacaag aaaatagtac aagtggaggt gcttgaagtc
     5821 catacgcaat agccactgct atagctggcc cagtaacctg tttagcaaga gggcttataa
     5881 cttcagtaaa aagagaaata ttgaaattat ctcctaatgt attgaaaatt gttccaatta
     5941 aaagggaaga aaaaagaccg agtgccatat aacttagtgc gtcaataaga tacctttgaa
     6001 cagaaatttc aatgtttttt cttttagcaa atgatttgaa atttcttttt ttataattga
     6061 tttccaattt aaaatcctcc tcatataata taagtataat ttctttttaa ttaattatat
     6121 aacttttcag ctgacaagac aaatgttatt tcactaaaat tagactattg taatagtaag
     6181 aaattaacat taattttagt tttaaatttt aaatttatgg tttataatta gtatagggat
     6241 attctgaaat ataataattt tccagtttat cccaatattt aacttaggta gattgcttac
     6301 aaaacaaatg attatatatt aggctaaaag gaagtggata gatgatattt gtaaaaaata
     6361 agaaatacag aaaaattgcc ataattatat ctattcctat attgcttgtt gcgcagtact
     6421 acctttttaa atttggaata cttagaccaa tggtaaaggg agtagaaatt gaaattgtag
     6481 atggggaata tgttaaagat atagataaat atgtagtaaa attaaatgaa acagttactt
     6541 tgtctacagg tgaatatatt aaggttcctt cgtatgctaa agatccaaat atttggttta
     6601 atgtattgga taattcagga actttaaaaa tagatggaaa taaaatgaca gcattaaagg
     6661 ttgggtattc ttctgtgggt atcatgaaaa attcaagagt attaaaaaag gctactataa
     6721 aagtagtaga cccagaggta gagtcactag atatggagat taatggggat ttaaaacatg
     6781 ttggtgatag tgctactata gaaagcacaa tagaagttga ttatgataag tttaaaaaat
     6841 catataagcc agtttataag agtagcaatg aaaatatttt aaaaatcaaa ggaaataaag
     6901 taagtgcagt tggagttggt aaagctacaa tatatgctaa gtgtggaaat aaagaaattg
     6961 agagaacatt taggattcaa gctagagttg ctgaaataaa tatggatgat ataaaaatag
     7021 aagttgacca aaatgaaaaa ttgaaaccag atataataac ttctccacct ggcttagaac
     7081 caccaaaaat atattatgag ttagtagatt caaaattacc tgtagaaaga gcaataagtt
     7141 tgagtagtaa tggaaatgtt gtaggcctta gagaaggtaa agatagggtg agaataactt
     7201 gtggagataa atctaaagta ataacagtaa cagtagtcaa agaaactata acgaatgact
     7261 atatacaaaa cttggttggt tcacataaaa tagtaaacaa taaagttatt attaaacttg
     7321 aatgggatta tatgaaagat gtctttgatt atgatatata tcttaaagat aagttgtcag
     7381 gagaatcatt taagaaaatt aaatctataa ctataaatga aaatgattta ggtaagtcta
     7441 acaaagtata tgcaactata gaagtagata tgaagggtaa tcaaaatgta gattttgata
     7501 tttatgttat agggaaaaag gattcagata caaccaagcc aagtaatgta ataaatatta
     7561 ctaatggaaa tggaaattca aataatgata ataatattaa agacatgagg gtagaaaata
     7621 tatcggctaa tgttgataga gagaataatg tcattagagt taactggagt agtatatctc
     7681 ataaggactg tacttacagt atatatgtaa aaaataatac taatggagat ggagaattta
     7741 ctctatatca aagtgatata cagggaaatg agcatactat aagtattcct gaaggagatt
     7801 taaatctaga tatttatgtt gttgcaaatt ataatggaaa tagctctcaa aatagtgatg
     7861 ttataaatgt aagatagatt tgtatacaaa ttgaaagtgt tgagtttatt taaa
//
LOCUS       DAEMAE010000169         7909 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEMAE010000169 DAEMAE010000000
VERSION     DAEMAE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.171"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            216..587
                     /locus_tag="KPH93_002845"
     CDS             216..587
                     /locus_tag="KPH93_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689639.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            complement(584..724)
                     /locus_tag="KPH93_002846"
     CDS             complement(584..724)
                     /locus_tag="KPH93_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689640.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(1382..2278)
                     /locus_tag="KPH93_002847"
     CDS             complement(1382..2278)
                     /locus_tag="KPH93_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1689641.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLTDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKIVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFEGIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     IFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            2420..3847
                     /locus_tag="KPH93_002848"
     CDS             2420..3847
                     /locus_tag="KPH93_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1689642.1"
                     /translation="MTNSFKKDNNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQAGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILSALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSNCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILISFYLFKYNKNEQ"
     gene            complement(4044..6638)
                     /gene="clpB"
                     /locus_tag="KPH93_002849"
     CDS             complement(4044..6638)
                     /gene="clpB"
                     /locus_tag="KPH93_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG1689643.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETVIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(6675..7271)
                     /locus_tag="KPH93_002850"
     CDS             complement(6675..7271)
                     /locus_tag="KPH93_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689644.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSRYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENIYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(7288..7692)
                     /locus_tag="KPH93_002851"
     CDS             complement(7288..7692)
                     /locus_tag="KPH93_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG1689645.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKSNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2622 a         1231 c          822 g         3234 t
ORIGIN      
        1 tcaaaagttt ctattgtatt tattaattaa tttagtttct tatgtcaaca taccacctct
       61 catcaaatta ccatttgtat aatttatatt atacatatac aaatggtaat tttcaatata
      121 ttttattatt ttaacccttt ttttttatat ttttggtaat tttattttac tttttataaa
      181 aataacttat aatttcggta aggagtgatg tacatatgga aagtttagga gatagaatag
      241 caaatctacg aaaagaatta gatataaatc aaaaagaact tgctactaaa gtcggtataa
      301 ctgaggctag cttgtcacgt tatgaaaata atttaagaga acctaaaagt gaaataatag
      361 ttagattagc aaaagcatta gaaacatcaa cagattattt attaggcgta aatgataata
      421 caaaaataag caaagaagat aaattaatca tagagaactt atcagtaagt gaaaaaacaa
      481 aaaaactctt agaaaaaata tattctcttg aaaaagaaga tagagaagct atagaaaaaa
      541 tgattgataa tgcctattta aaaagattcc ttaaagaaga gaattaattc tcttcttttt
      601 ctttatacaa aatctcaagt aataattcat aaaaagtaat atcttcactc aataaatgtt
      661 ctaatttcat tcctatatct tcaatttttt ctttttttgt aacagtagtg tttgactttt
      721 ccacatatac catcccccaa caaacttacg ttcttaatgt tagtcagaaa ttcccaacaa
      781 atttttaata aagtttatgt aattgtattt tgattttata aaatatattt tgattatcca
      841 aaatttccct agataattat cttaattata taataatact tttattctac agttgcaata
      901 aaaaactcgt aataataaca aaaaaatcta ttattgtaga atcttgtatg ttttttctat
      961 ttttatatat ttttcaggat gatttttatt ataaaatttt ttataaatat ataatttttt
     1021 tatatttatt tgactttctt ttaattcttt aggatggatt taaattttct ataaaaatca
     1081 tactatttta gctgtttact tgcttattta atagttttga ttaaatatct ttcagaatct
     1141 aagccagatg tctaaaaaaa aacaaatttg taatataatc attttttctg ctattcctac
     1201 ttttccaact ttagtttcat agtaaagtac acttttcata gacactacat tctttcatac
     1261 tcattttcac atttaactat acaaatagtc ttactcattt acatcctata ctttattaat
     1321 atttattaag aaaaactcaa agatttaaaa atatatattt tctataaagt atatttataa
     1381 attatttcat atcttctttt aaaataacat tactaacttc tctatgttca tttctatact
     1441 tagttggaga tatattataa ttccttttaa ataagcgact aaaataattt atgctatcaa
     1501 agccgcatct catagcaatt tctgtcatat ttaaatctgt ttcctttaaa taatatatag
     1561 ctttatcaag tctgattcca tttataaaat cgattgtagt cttacctgtt atttgcttaa
     1621 atattctaca aaaatgatgt gcactcaagt tcacacgact agcaagttct tttaatgtaa
     1681 ttttatccat atatttttct tctatcaaat caaagatacc ttcaaatttt tctaaagaat
     1741 actttctttt tataaattca tcttcagaat atattttatc aatatgactt cttaataaga
     1801 gtactattaa ttgatagata ttagctttta tagataactc atatccaact tctttattgt
     1861 gatattcatc aagtatagtt gtaatacatt ttaaaatcag agaatcattt tgaattagat
     1921 tattaaatac aatatgattt aaagccaatg gtgctaagta cttagtttgt aacaaatcaa
     1981 cttgattact aaaaaggaat gtgggttcaa tacgaatact atagaatttt aaattatctg
     2041 ataagctttc taaataatgt aattcattac tatttgctat tacaatgttt ccaggcacta
     2101 tcttaaaatt atccaatcca cattcaagat atgctatacc atcaataaaa taatatattt
     2161 gtaaatgctc atgccaatgc cttttaaagt ttgctccttt tttaatgtca tccgtcaaaa
     2221 aaatatctat aaagttttct tgacctttcc tgttaaaata tgtattttta tccttcatta
     2281 ttcccctctc atcaaaatag tgctaattat aagcaatata gttattgtat tcattacttc
     2341 atatcgctat tatataaaat gtaactatca ttgtaaacat taacataatg ccagtcatta
     2401 taataaggag gtaataaaaa tgacaaactc ttttaaaaaa gataataata gaaaaaaaat
     2461 cacagtttta tttgtagtca ttgccatgac ttttatggca acattagatt ctagtattat
     2521 aaatgttgct ctcccagttc ttgcaagcaa actaaatgta tctctagcat ctattgaatg
     2581 ggtaatagca agttactcaa ttattatttg ttcaacacta ttattttttg gaagacttgg
     2641 agatattatt ggtaaatcaa gagtattcca agcaggcact attctcttta catcagcttc
     2701 attactatgt ggattaagta attctttaac tttacttata gtttgccgct ttattcaagg
     2761 aataggcgct tctgcctaca tggcaaacaa tcatgggatt atcacagaac tatttccaaa
     2821 agaaagcaga ggaaaagccc tagggattct agttactgca gttgctattg ggaatatggt
     2881 tgggccatct gtgggaggat ttattttgtc aatatttgat tggaatgtta tattctttat
     2941 aaatattcca atcggtctta tcgtaatctt tttaaataca aaatttcttc ctaatagtaa
     3001 aaaatcttca gaaaatatgg ataaaactgg tgctatactt caatttttag gaacaacatt
     3061 attttttagt gctctaatta gtgctcaaca aacaggatta ctaaatccat atatattaag
     3121 tgcactatta ttatcaatta tttttattat attatttttg atattagaaa aaaaacatcc
     3181 tcaaccgctt cttgatcttg aaatttttag aaattttaaa ttttcattga atttaatttg
     3241 tgctcttaca tcattcattt gtattgcatc atcaagtatt ttaattccat tttatttaca
     3301 aagtactatg aaacttcccc ctattcaagc aggtttattt atgatactat cgcctttgat
     3361 tttagccatt ttttcaccta tatttgggaa tatatcagat aaaattaagt cagaaaaaat
     3421 tattttaatt ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa gattgaaaga
     3481 aagttctgcc cttattttat ttgtaattta tatatcgata atatcaatag gacaagctat
     3541 atttcaacct gctaacaatg ctttgataat gtcaaactgt tctagaagta agctaggagt
     3601 tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa gttataggta ttactatttc
     3661 tacaacactt ttatataatt ttatgagtat taaagctgga tatagggtca atgattatgt
     3721 gattaataat gataaaatat ttgtatttgg aatgagaaat gtttatatta ttgttacact
     3781 tgtttgctta ataggtgcaa tattaattag cttttatcta tttaaatata ataaaaatga
     3841 acaatagttt tttataatta tattcaaatt atgattatat tataagaaac ttactaaaat
     3901 actcccttag agttaagttt aatttgaaca tattaactta aaaacatttt attagtttga
     3961 taaaatattt cttttactat agtttataaa taaaaatcag ctgagcaaaa tatttggtat
     4021 attatgctca gctgattctc ttattattta actaaaactt caatattttc atcttttcca
     4081 tctataacta tagtacatcc attatacaca tctccagcaa taagtttctt tgcaattaca
     4141 gtttctaatg tatttcctat atatctctta agaggtcttg ctccatatac agggtcatat
     4201 ccttctctta ccataacatc ttttgctgaa tttgtaactt caatagtaat atctttttct
     4261 tttaatctat tttttaaatc tttcataaat atatctataa ttttcttgat attttcttgg
     4321 tctagaggct taaacattat aatatcatct actctattta aaaactcagg tttaaatcta
     4381 tgcttcattt cattcattac taaattgtta gtagtctctg ttatatttcc tcctgcttct
     4441 aatagataat tacttcctat attagaagtc attattataa gagtattttt aaagtctact
     4501 gtcttacctt tattatcagt aagtctacca tcatctaata tctgtaagaa catgttaaat
     4561 acatcctcat gtgctttttc tatctcatca aatagtatta cagaataagg agcacgtcta
     4621 actgcttctg taagttgacc accctcttca tatcccacgt accctggagg aggtccaact
     4681 agtcttgata cagcatgttt ttccatgtat tcagacatat ctattcttat tatattatct
     4741 tcactatcga ataaatttct agcaagagtt tttgcaagtt cagttttacc aacacctgta
     4801 ggtcctaaga aaataaatga accaattggt tttctttcat cttttaatcc tgctcttgca
     4861 cgtattactg catttgaaac tgctgtaact gcttcatctt gtccaataac tcttttatga
     4921 agttcatctt ctagtttaag taatttttct ctttctcctt caactagttt agttactggt
     4981 atacctgtcc actttgatac aatgctagat atttcttctt cagtaacttc ttcttttaaa
     5041 agagaatttt cagaaccatc attcatattt tcttcgtact gctttatttg ttcttctaac
     5101 ttaggtattt caccatattt aacctctgct gctttgttaa aatcatattc tctttcatac
     5161 ttttcagctt tacctttagc ttcgtctaat tgagccttta agtttttaat atctaatatt
     5221 tgactctttt ctttttcata ttttgctgtc atttcatcat ttttagattt taactctgct
     5281 atttcttttt gaatatcatc caatcttgat ttacttttat catcattttc tttaagtaat
     5341 gcttctcttt cagtttctaa tgtaaataac tttcttctaa caacatctaa ctctgttggt
     5401 aatgaatcta tttcactacg aatcatagct ccagcttcat caattaaatc aattgcttta
     5461 tctggtaaaa atctatcttg tatatatcta tgagaaagtt ttgccgctgc tacaatagca
     5521 ttatcatgta ttcttactcc atggtgtatc tcaaatcttt cttttaaacc tctaagtata
     5581 gatattgtat cttcaacagt aggttcctct gcaattacag gttgaaaacg tctttcaaga
     5641 gccttatctt tttctatata ctttctatat tcatcaaaag tagttgcacc aatacagttt
     5701 aattcacctc ttgcaagcat aggtttaata agattacctg catccattga accttctgtt
     5761 ttaccagctc caacgatagt atgaatctca tctataaata gtataatttt tccatcagaa
     5821 ctttgaactt cttttaagac tgcctttaat ctttcttcaa attcacctct atattttgca
     5881 ccagcaatta aagcacccat atcaagtgag aatacaattt tatcttttaa accttctggg
     5941 acatctcctc ttacaattct ctctgctaaa ccttctacaa ttgcagtttt acctacacca
     6001 ggctcaccaa tcaatacagg attattttta gttcttcttg ataaaattct tataattctt
     6061 cttatttctt catctcttcc aattacaggg tctaatttat ttttcttggc taaatctact
     6121 aagtttgttc catatctagc aagtgcatca tatgttcctt ctgggtcttg tgtttctacc
     6181 ctttgactac ctctcacttg tgataaaata tttaaaaagt cattcttatt tatattatat
     6241 tgttttaata tcttgccaac agctgatttt gattctgttt ccatcattgc aagcataaca
     6301 tgttctacac ttatatatga gtctttaaat tctttagaaa tactctctgc ctttataagc
     6361 acctcattta tttttctagt agctgatact ccttgtgagt cagcaccttc tccatatact
     6421 tttggaagtt tatcaatttc aaagtttaca gtatttctca aagaatcaat tgatatattc
     6481 attttttcga ttatatttgg tatcaatcca tcctcttgat taataagtgc tgagaaaaga
     6541 tgaattacat ctacttgttg gttatgattt tttactgctt cgttataagc ctcatttaaa
     6601 cttttttgaa ctcttaaagt cattttttct acgtccatat aaatccctcc attttaatat
     6661 tatatccaaa taaattaaat tctctcattt ataattattg attttctaag ctctaaaaga
     6721 ctatcaagta aattctcatt tttttgtata tcgaaactca atgttaaatt aaatgttcca
     6781 tctgaattaa aaaaatcgaa tacttctttt gattttgcta ttaaatacaa gtcttcatct
     6841 ataagtcttt tttcacctat taataaatac tgacatttat ctataaagct ctttttaaaa
     6901 tcaatagtgt atatattttc taaaatatta tattgataaa tagcaatatt attaaacact
     6961 tttaatatgt ttgaatttac aaaattgtaa atttgattat tgttatacag tgatatttgt
     7021 tcgtatatat attccctaaa taaaggatga atgttttgct taagttttaa agtaacttca
     7081 gctggtattt caaaataaag cttccagtac ctagagttat ttttttctaa aataaaagct
     7141 actttgttca aactggtaga actaatctcc tttagtgtac aattaaaatt atagtgtctc
     7201 aaactagttt tttcattctc aaatacataa tcaaaccact gtagaacatt tctatcaagt
     7261 tttaaactca tataaaaacc ttcttcctta tttactaacc ttattaggtc tttttatctg
     7321 attatattct gctaataaac tttctttatc agctctacta cctttatatg ctatatgtgg
     7381 atttatcata tactcaccat ttttcactct ttgtaaaaaa tccatttttt tcagctccgt
     7441 aaacaatgaa gataaagaag tttttggata ttcaaattca caagataatt catataatgt
     7501 ttttctaatc ttattacttt tatccataga gtttattaat tttaatggaa ttaaatccct
     7561 cttagtatac attccttcta ctactttaat ataattctta aaacaaacat tcatatactt
     7621 acttttattc ataactaaac ttttactctc tgtatattcc tgatttaaat ccttttttaa
     7681 tgtcttatcc aaattaaata tctcttctca ctctccttat ctctaattga taatataaat
     7741 atattacaaa aaaatatgta tgtcaaaact gaaaatacaa aaagtttacc ttataagtaa
     7801 acttttattt agctataagc taaactttat tattttttta atttagtatt tccaatttat
     7861 tggcaggatt tttgaataat atttataaat tttttaaaaa aatttaaat
//
LOCUS       DAEMAE010000170         7891 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEMAE010000170 DAEMAE010000000
VERSION     DAEMAE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.172"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..276)
                     /locus_tag="KPH93_002852"
     CDS             complement(<1..276)
                     /locus_tag="KPH93_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1689646.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLA"
     gene            complement(289..1020)
                     /gene="chbG"
                     /locus_tag="KPH93_002853"
     CDS             complement(289..1020)
                     /gene="chbG"
                     /locus_tag="KPH93_002853"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG1689647.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVIKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDV"
     gene            complement(1046..2356)
                     /locus_tag="KPH93_002854"
     CDS             complement(1046..2356)
                     /locus_tag="KPH93_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG1689648.1"
                     /translation="MKNGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELKEFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(2454..3767)
                     /locus_tag="KPH93_002855"
     CDS             complement(2454..3767)
                     /locus_tag="KPH93_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1689649.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMSSIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(3789..4100)
                     /locus_tag="KPH93_002856"
     CDS             complement(3789..4100)
                     /locus_tag="KPH93_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1689650.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(4300..5004)
                     /locus_tag="KPH93_002857"
     CDS             complement(4300..5004)
                     /locus_tag="KPH93_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1689651.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(5099..6460)
                     /locus_tag="KPH93_002858"
     CDS             complement(5099..6460)
                     /locus_tag="KPH93_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1689652.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTMSLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     TIITILTGARVPLAYFISRPEILGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            6955..>7891
                     /locus_tag="KPH93_002859"
     CDS             6955..>7891
                     /locus_tag="KPH93_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1689653.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKNMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEICGDINKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQAGDLYIKQASKIIQYVINKKYIVYRIGGDEYII"
BASE COUNT         2636 a         1408 c          880 g         2967 t
ORIGIN      
        1 tgctaattgt tgaaaattca tagctgtcat taaatggtct tgtgcatgaa taagtataac
       61 acttatttca gttttattac cattagcttc attttgtatc aattctgttt ggaatttatg
      121 agctttatga attgattctt tcgacttttc aatacattct ctagctttat ctatattccc
      181 gctctttgct tcttttattg cttcaaatgc tagccctttc gcatcacctg caaatccaat
      241 tattccaaaa gatatctcta tcatcttctc atccatatat tcgacctcct aaacatctct
      301 atagcttgat atgactaatc cattcttttc taaaaattct ttgacttttt tagatgtaag
      361 tatcttgtgc tctttcgttc tatcaatagc ataagatgta gaatttaaaa tataatcatc
      421 taaaaaagct gggtgagaca taatatccac tacatcatac ttcattattt cgtctatatt
      481 ttttataaaa tattcttcac ttacattttc cttataaaaa ctatctatta aaggaactac
      541 tttagaatac tctaaattat attcaaatcc acctcttatt ggaagattat actttattac
      601 aatcttctct attactggct ttaaacttac taaagtatgg atatgatggt gactgtctaa
      661 atgagatata tctattccaa gcccttttac tctatctatt tgagcacaaa gttctctata
      721 aatctcatca caatccattt tttcaataat ttcgtctgta atcctcctaa taaaaaaacc
      781 gtctttatca acaatagttt ttaagttaga taataatggt ctaccacaac ttaaagtcat
      841 atgaactcca caatttaaag ttttattttc tttaagtaag cccactccat gttctacccc
      901 aggcatattt gccatcatag aagtagacct aactatacca ttattatgag ctgaaattat
      961 tccatagttc acagcttcac aatatccaaa atcatctgca tttattataa ttttagtcat
     1021 accataacct actttctata gacaactatc tgttaaatct aggaagataa tctttatgag
     1081 cttccaacat ttcgtctaat atagtttttg ctaagtcatc agatggaatc aatgggttta
     1141 tacaaagtgc cagaagagca gattcataat cgccatatac tgctgcttta gctgccaata
     1201 cttcaaatga tttgatttgg cttactaatc catgcacaga atctggtaaa tatcctatac
     1261 ttattggttt tggaccattt tttgttatta cactactcac ttctactgct tgttcatctt
     1321 taaaatctct tatagaacca ttatttaatg tattaactac ttggatatct tttttgtcat
     1381 tgtatataga acttattaaa ttacaagcag catcactata gtaagcccct cctctttttt
     1441 caagttgagg tggttttaca tctaattttt catccttata taattcaaat aattgttctt
     1501 ccaattcttt aactacttga cctctagctt ttccctcttt aaattctttc aactcatcct
     1561 caagcatttc tttagtttta tagtagtatc tatggtatgg acaaggtatt gcacccaaag
     1621 attttacaaa ttcagcatta aaatcaattg cctttatatt ttgcatactt atatctgctt
     1681 tgacaaactt atcaattgct tcttttgtaa catcttctcc atctaaagat acatttagac
     1741 cataaaccat gtgattaaga cctgcaaaat ccatacttat tctgtcactt tctacattaa
     1801 atagtttggc aacatcattt tttaaatgaa ttggcacatt gcatagacct atatatcttc
     1861 taaaattagt atatttgaat acagcctctg ttattattcc tgttggattt gtaaaattca
     1921 ccatccatgc atttggacat aattcttcca catcttttat tatatcaaat ataactggta
     1981 ttgtgcgaag ggctttaaat aaacctccag cgccattagt ttcctgcccc ataactccat
     2041 gagataaagg aatagtttca tcttttattc tcgcatctaa aagacctact ctaagttgtg
     2101 ttgtcacaaa atctgcatct cttagagctt ctcttctatc taaagtaaga tttattttca
     2161 tatctactcc agcttttttt accatcctct tagctagatt tccaactatc tctaattttt
     2221 cttttccagc ttctatatct acaagccata attcctttac aggtaactct tcatatctat
     2281 ttataaaacc ctctacaagc tcaggagtat aacttgaacc tcctccaatt gtaactattt
     2341 ttagtccatt tttcatctca ttataccatc cttacctata atttaatata tttctacaaa
     2401 accctatata tttatttaaa accccataca tttatttacc gaaataaaac tacctagttg
     2461 ttaattttca atttattagc ttccattttt tcagatgcta tgacaaatgg aatatatatt
     2521 aatattgata atattaaatt aaatagtgct aaagctccac cagtccagtc acctgtggcc
     2581 attgctccac ctaatatagg aggtgttacc caaggtattt taggtaaaat tactggtgct
     2641 actaatccaa aatctattgc taagtatgat acagcactac atattactgg tgcaagtaca
     2701 aatggaatca taaatatagg atttaaaact attggaagtc cgaatatcaa tggttctcct
     2761 atattaaata ttgcaggtgg cccacctaat actatcattt ctttacgtct tcttccagct
     2821 attaataaag ctacaattaa tcccaatacc attccagaac cacctaaata tacaaatgca
     2881 tcaaagaatg cacctgcaag tacatggtat ccttccgttg ctcctgcttg agccaaggca
     2941 gcattttctc cacctaattt cataagtata gtttctgtaa aaggaagtgc tatatttgca
     3001 ccatgaagtc caacagtcca aagtatatga attataaatg caataagcat tgtagaacct
     3061 agactatcag ctacatttga tagtggtgct cctaaataag tatttaaaat atctgttaag
     3121 aaacttccat tagaaagcat ttttataaat attcctatca cagtaaatat cattatagtt
     3181 aacattcctg gtattaactt tgcaaatgat cttgaaactg ctggtggaac tccatctggc
     3241 atttttatag ttaatttctt tactttagat aatcttacaa atatctcagt tgataataat
     3301 cctattaata tagaagagaa taaagctgct gtacctaagt aggtttgttg tatcattccc
     3361 catccttcaa ctactgtagt tcctccctca ggaactattt taccaatttg tggagccatt
     3421 ataaaaaaga tagataaagc tataagccca gattgtaacc catcactctc ataagatttt
     3481 gctagaaaat aagcaacacc tattactaag aatactgcca ttgtacctat tgctccccac
     3541 caaaggtcgc ctccaaatgt ggtccagttt tctccaaata tacttgacat caaatttttg
     3601 tatgcttcta atggtaagtt atttatcaat gttcccaatg cacctaccat agttataggt
     3661 atcattacta taaatgcatc tcttactgct actaagtgcc tttgagctcc tattttagca
     3721 gctacaggca ctatatattt atccataaat gacatgaatt tttccatttt acatcctcca
     3781 gtacaacttt attttgaact atttaactct acagctctat ctaacacagc ttttccatta
     3841 catcttccat aatccatcat atttataact tctactggaa tattatatgg ttttgccatt
     3901 tcagttgctt ttcctaatac atatctaact tgaggtccta aaagtaagac atcacaattt
     3961 tctatttcat tttttaagtt cacttctgat atagcccata tttttgcttc tataccgttt
     4021 tcctttgctg catcctccat ttttgtaact aataaactag ttgacattcc tgctgaacaa
     4081 gctaacatta tttttatcat tttttcccct ccaaattaga ttcattaaat attttttaag
     4141 tcataagttt tttaaactat acatttaaaa tatgcttttc tattaatttt ttcttaatgt
     4201 atatatctta tttttaaatg tatatacatt taattcttga tattattata tcccaaatgt
     4261 atatacattt caagaaaact ttttcatttt atttaaattt tattcgacaa cagcattcaa
     4321 agttaatgta tatccatagt gatatgtgtg tgagtactca aaaatctcac tattttgtaa
     4381 cttacaaatt tggcttatat ataaaagtgg ctctggttct tctaatttta gattatccat
     4441 acactcttca tttgatatta cagcttgtac ttctctctct gacatggcta tcttgtatcc
     4501 acacacttct tcaacaaaat tataaagaga attttcacaa tgagtcttat ttaaatttgg
     4561 aaataactta actggcatat aagtaatcat atatgaaact cttgcatcat tggctaatct
     4621 tactcttttt atttcccaaa cattttcttc taaacttata ttcaacttac ttgataaatt
     4681 ttcatctgcc tgaactattt caagagatac caatatatta gatactttat agcctttact
     4741 ttccatctct ttagtaaatc cacttacaga cgatatatta tcatatatat tatttgcaat
     4801 aactatacta cctacacctc tttgtctagt tatatagcct tctttaacta gattattaag
     4861 tgcagacctt acagtcattc tcgttacatt aaactcttcg ctcaactgtt tttcagaagg
     4921 gattaaatcc ccttccttca aactgtctga atttattaac tctctaacat gattttctat
     4981 aactttataa attggctctt tcatctaatt cactccttat taattaaatc ttttatataa
     5041 taagataatt ttatatacaa acaaagaaaa tgtctataca tttaagataa attaaatttt
     5101 attcagacat cttgtacaac tttccgagtt ttaataaaga tataaataga cttatcatta
     5161 atatgccttt taatatactt gataaggtta tactccacca tacaccattt agacccaata
     5221 tttctggtct agatataaag taagccaatg gtactcttgc accagtcagt atagtaatta
     5281 tagtagaagg tatataggtt ctccctaatc ctttgaaagc tcctgttgtt attatttcta
     5341 aacacataaa aaattgagaa tagcctaata ttttaagata aattgctcct ttttctattg
     5401 cctcactttc atctataaaa atagaaaata tactctctcc tgcaaatact aataccaaag
     5461 tagttattat cccaagtaca gaagatacag ttatagctat tttacaacct ttatttattc
     5521 tagaatactt cttggcacca taattttgtc caacaaaact gcctaatgca actgatagtc
     5581 cttcagctgt catccaggaa attgcctcta tttgcgagcc tactttttgt actgcaacag
     5641 ctacaggtcc ccatgatgct actattactc ctaataacat tgagaatact gtaaacatac
     5701 cactttgtat tgctataggt aatcccagtt tatacagaac tttataatat ttagtctcta
     5761 tttctttaaa tagccttaat ttaaaatatt cttctttact tttaactatt atatatataa
     5821 aacatgtagt aactactatc tgagcaaata cagttgcaat agcagctcct gcaacaccaa
     5881 gttttggagc aaatccccat ccaaaaatta atatagggtc aagtataata ttagttatta
     5941 atcctactgt attaatacga aaaggagttt tactatttcc aagtccgttg aaaattgcag
     6001 taaatactgg attaataaaa taaaatacca tacctagagc tactattatc aaatactgtc
     6061 tagacatggt tataacttct aagtctccta atctaaaaaa acctatcaat tgtttattaa
     6121 gtactattaa agacattgta tatattattg ctagtataat atttatttct attgctgact
     6181 tcatatatga tttagtagtg cttatattat ttgcaccaat actttgtgct acttttatct
     6241 ctccacctac ttttgaaatc ataataaatg ccattgcaag ccatggaaaa aatccagctg
     6301 ttccaactgc agctactgct ttacttccag ctttaccaac ccatatcata tctatcatgt
     6361 tataccccat ttgtataaat gaagtcccca ttattggaag tgatagttta agtaattttt
     6421 ctgtaattct tccttctgtt aaattaagtc tgtctttcat tttccacctc ttttatatta
     6481 aatttatttg cttatatagc tagattaatc attttatttg attgagttca ctatccttca
     6541 atttatatta tcatatctca gaattgcatc tcaaccatac ttatgtttaa gataaagtat
     6601 tttacctatc tataaattaa tacttatgaa tataaatcta gccatttcat caatacatat
     6661 tataaccctt aacttaactt caattaagta tacatattgt aatcaattag aatattttat
     6721 actgtatttt cgattaagaa atgacaaata taaatgattt atctataatt ttatttatac
     6781 ataaaatagt atactataat aataaaaaaa ctatatttga atactttaag catgtttact
     6841 aattgtaatt tccacacaaa tttgataata tatgaatata ttatggtgaa atttatagat
     6901 atatattaat tatggggaga ttgtgggagg ttcttttcac aagacattta agctatgaaa
     6961 ataaataaaa aattcaatag agttgatatg caggtttcaa tacttactgc aattattgta
     7021 ataatatctt cctcatgtgt atattttttt aattatacac taacttataa gaacatgata
     7081 tactctttaa gtgagaggtc aaaacatatc tatgaatatg tagaaagaaa tattgataaa
     7141 aataccttta ctgaaattaa ttctaaagaa gaccaatcaa aaaagtctta taaaagttct
     7201 aaaatcctat tagaatctgt aaaaaatact actggagtta tgtatctata tactgctaaa
     7261 aaaacaaaag atggatcact agtatatgtt gttgatggtt taaattcatc tagaagtgat
     7321 tttagaaatg ctggtgattt gatagaaaaa gagatttgcg gagacattaa caaagcatta
     7381 gataatcata tattgttacc taaaaaaatt aaatcaacat cttggggata catatttata
     7441 tcgtattttc ctattcataa tgatgttggt aaggttgttg gagtaatagg aattgaattt
     7501 gaagcaaaac atcaatacaa tacatttaaa tttataagta tagttacacc aataatagca
     7561 ttattaactt gtctaatatc agcattgatt gcagtgattc tatttaaaag aatatctaat
     7621 cctacatata ctgattttgc taatacagat tatttaacag gactaaaaaa tagaaatgct
     7681 tttgaaatag atatgaataa cttaaacaat tcaaatctaa agaatctaat atctatcata
     7741 tcaatagatt tagatggttt aaaaaagatt aatgataaat ttggccatca agcaggagac
     7801 ttatatatta agcaagcaag taaaattata caatatgtaa taaataaaaa atatatagtt
     7861 tatcgtattg gaggtgatga atacataatt a
//
LOCUS       DAEMAE010000171         7890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEMAE010000171 DAEMAE010000000
VERSION     DAEMAE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.173"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            51..935
                     /locus_tag="KPH93_002860"
     CDS             51..935
                     /locus_tag="KPH93_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG1689654.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKSYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHTLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1034..2209
                     /locus_tag="KPH93_002861"
     CDS             1034..2209
                     /locus_tag="KPH93_002861"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG1689655.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2337..3191
                     /locus_tag="KPH93_002862"
     CDS             2337..3191
                     /locus_tag="KPH93_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689656.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKDGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3235..4470
                     /locus_tag="KPH93_002863"
     CDS             3235..4470
                     /locus_tag="KPH93_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG1689657.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVISICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKNFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAINSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4572..5513
                     /locus_tag="KPH93_002864"
     CDS             4572..5513
                     /locus_tag="KPH93_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG1689658.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVVRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGKSTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYIASGDDLVYALVASPLAKNAPVA
                     LVSNKSDKSILSGANKVTAIGISDNSVIEQCLNAVKK"
     gene            complement(5787..5947)
                     /locus_tag="KPH93_002865"
                     /pseudo
     CDS             complement(5787..5947)
                     /locus_tag="KPH93_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter"
     gene            complement(6064..6528)
                     /locus_tag="KPH93_002866"
     CDS             complement(6064..6528)
                     /locus_tag="KPH93_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689659.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDINIEEIQEAINKTSEETEEKINWTNAWGKKYPILLRY
                     QTDVNIPNYAYKVNMMLEELKQEYQYNELEAMLVLKDILYNVWKNRKSKRKQ"
     gene            complement(6850..7080)
                     /locus_tag="KPH93_002867"
     CDS             complement(6850..7080)
                     /locus_tag="KPH93_002867"
                     /inference="COORDINATES: protein motif:HMM:NF024076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HBG1689660.1"
                     /translation="MIDIQDKKYCLALEEVIEYVNNPVFTQFCSDMKSKYNCNEKIEF
                     SSCSWEFGWNIKLKKSGKNLCTVYLKEKYFTF"
     gene            complement(7366..7719)
                     /locus_tag="KPH93_002868"
     CDS             complement(7366..7719)
                     /locus_tag="KPH93_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG1689661.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKSDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         2897 a          930 c         1336 g         2727 t
ORIGIN      
        1 ctagtaaaaa ttaaataata ttaatcaact tattgaatgg aggaataaaa atgaaatttg
       61 tgactttttg tagtagcaat gaagaaaaaa taggtgtatt taattcagaa acaaatagta
      121 tatatgaaat taatagttta ggattaagta aatcatatac tgatatgaat gattttattg
      181 aaaatgtatc tacaggagat ttagaaaaaa taaaaaacaa ttcatttgaa aacgcaaagt
      241 gttataagtt agaagaagtt aaattatgtt ccccaattgt aagacctaaa aaagatatta
      301 tatgtttagg tctaaactat aaagaccatg tcaatgaaat tccagatgga gttataaaaa
      361 acgtagttat gccagattat ccaatttatt tttctaagag agcagacaaa ataattggag
      421 tggatgataa gataagctta catggagatt tggtagagaa gttggactat gaatcagaac
      481 ttgcagtaat cataggtaaa gaaggtataa atatatcaaa agaagatgct tatgagtata
      541 tatttggata tacaatagtg aatgatataa gtgaaagagc gcttcaagat aaacatgttc
      601 aatggtttag aggaaaaagc ttagacacac atacatctat gggaccttgt atagtacata
      661 aagaagaatt tgaacatact ttaaaattag atataagtag tgtagtaaat ggagaagtaa
      721 gacaaaattc taatacagag tactttatat ttgatatacc tactataata aatgatttat
      781 caagaggaat gactctaaag ccaggagata taatttcaac tggaactcca gcaggggttg
      841 ctatggggat gaatcctcaa gtatatttaa agcatggcga tatagttgaa tgtaaagtcg
      901 aaggaatagg agttttaaaa aatatagttg attaatggat aaatatgcca tataatttta
      961 ttagtacgaa taatttaatt aatataggat taatcaaatt tatgattaat ttattataga
     1021 gagggatgag tatatggcaa gagaactaga attaaagtat ggctgtaatc caaatcaaaa
     1081 accatcaaag atttatatga aaaatggaga acttcctata gaagtattaa atggaaaacc
     1141 tggatatata aattttttag atgcatttaa tagttggcaa cttgtgaagg aattaaaaga
     1201 agctacaggt ctcccagcag caacttcatt taagcatgta agtccagcag gagcagcagt
     1261 aggagtacct ttatcagata ctttaaagca aatatatttt gttgatgatt tagagctttc
     1321 tccattagct tgtgcttatg caatggcaag aggagcagat agaatgtctt cttatggtga
     1381 ttttatagca ctttcagatg tatgtgacaa agagactgca actattatag caagagaagt
     1441 ttctgatggt ataatagctc caggatatac agatgaagcc cttgaaatat taaaaggaaa
     1501 gcgtaaaggc aactataata ttgtaaaaat agatgaaaat tatactcctg agcctattga
     1561 aacaaaggat gtttatggaa ttacttttga acaaggtaga aatgaaatat taataaatga
     1621 agacctatta aaagatattc caactgataa taaaattttt acagacagtg caaaacgtga
     1681 tttaattata gctcttatta cattaaaata cacacaatca aattctgttt gttatgctaa
     1741 ggatggacaa gtaataggag taggtgcagg tcaacaatca cgtattcatt gtactagact
     1801 tgcaggtaat aaagctgata cttggtatct tcgtcaacat cctaaagtat taaacttaaa
     1861 gtttaaaaag gatataggtc gtccagatag agataacact attgatgtat atctttctga
     1921 tgattatatg gatgtgcttg ctgatggcat atggcaaaac ttttttgaag aaaaaccaga
     1981 gcctctaact ggagaaggaa aaagagcatg gcttaaaact ttaacaggag tagcacttgg
     2041 ttcagatgca tttttcccat ttggagataa tatagaacgt gcaaaaagaa gtggggtaag
     2101 ttttatagca caaccaggtg gttctattcg tgatgataat gttatattaa cttgtaataa
     2161 gtataacata gtcatggcat ttactaaaaa tagattattc catcattaaa atactgaaag
     2221 tataaatata atatagatta agtaaaaatc tacaacaagt tttgttgtag atttttttat
     2281 ttgctgtata attttaaata gaactttatt tttagatatt caatttggag gttgatatgg
     2341 ctttaaatat aagaaactat agcctattat gtaatcaagg aacagctaaa tttaatgaag
     2401 atgtagtcgg tataaatcca tttggggcat gggttttaga tggtgctaca ggacttaatg
     2461 gaaaaaattt agtgtcaact gaaagtgacg caaggtggta tgttcagtgg tggaataggt
     2521 atttatataa aaatataaat aaagaagaat cattaaagag tattattaat actggaataa
     2581 tagaagtaaa aaaagagtat gaactaagat taaatgggtt taaggttgaa aaattagatt
     2641 taccatctag ttctatagtt gttgtaaaat ttcatgaaaa taaagtagag tattttttat
     2701 taggagattg tgccttgttt attaaaaatg gagattccaa agttataaaa gataggagta
     2761 tttgtaaatt tgataatact gtatttgatg aaatgagtaa actttctaac ctggaagaga
     2821 tggcatttga tgagattaga actagtgtta tggatacaat agtaagcaat aggttgaaga
     2881 aaaatacgaa agatggctat tggatattgg attttgatga ggaagctgtg gaaaattcta
     2941 tatatggata cattgaaata aaaaaagatt ttcaattgat gctaacgagt gatggtttta
     3001 caagtgcatg tgatagatat aatctaataa aagaagaaga attgatacgt atagcagaga
     3061 agtttggtat aggatatata cacaataaag taagagattt tgaagataat gactataaag
     3121 cggttaaagt gccaagattt aaagtaaaag atgattcatc ttgcttatat ttagatatat
     3181 ataatgatta gactgtataa tactatttaa ttttagaaag gaggcaagtg aaaagtgaaa
     3241 attcttcata taataacaca aaaaccaaat agtacaggta gtggtatata tttgtctggt
     3301 atgataaaag ggttcgaaaa aataggtcat aagcaagctg taattgcagg tatagatgta
     3361 aatgatgatg taaattgttt tccaagtgaa gtaagttttt atccagtaaa gtataattgt
     3421 ggggagttaa attttcctgt tgtaggtatg agtgattcta tgccatatga aagtacaaga
     3481 tataaagatt taaatataga catgataaat agattgaaat atcaatttaa agtaaatata
     3541 gataaggcta tgaatgattt taaaccagaa cttataatat gtcatcattt gtatctctta
     3601 actgcatttg ttagggaaat agtaaaagat ataaaggtta tatctatttg tcatggaact
     3661 tgtttaagac agttaaatac cattgattta gagaaagaat acataatagc aaatataagg
     3721 aaattagatt taatatttgc tcttcatgaa aatcaaaaat atgatattat aaagaatttt
     3781 ggagtaagtg aaagtaaagt agtcgtaatt ggtagtggtt ataatgatga tatattttat
     3841 aataaaaatt ataaaataaa ggatgataaa ataaaaatag tatttgcagg taagatatgt
     3901 aaatcaaagg gactgattcc atttataaaa gctatatcta aattaaaata tagtaaagat
     3961 ttaatagaag tcaattttgc aggtactgga agcgatatcg aatcatataa tgagatagtt
     4021 aaactggcaa gtaagtctcc atttaaaatg aatttcctag gaaagttaga acaaagagat
     4081 ttagctgagc tattcaacag gtcacaaata tttgtgttac catcatttta tgagggatta
     4141 ccagttgtgg ttttagaagc tttatcttgt ggcacagatg taatcactac tgatattctt
     4201 ggtgttaaag agtggattgg tagtgagatt aataactctg gaaagataga atatgtttct
     4261 ttacctttta tggagaaaga aggtattcca aaagatgaag agttatatga ttttgaaaat
     4321 aatttgtata atgcaataaa ttctaagata caatctttat tgaataactc aaataagaaa
     4381 acatctgtag atatgtcaaa aaaaacgtgg gatggcttag catatagaat aaatgaagtt
     4441 attttaatgg atgaattgtg tttagtctaa aattatttaa attataaaaa aagtgtaact
     4501 atttttacac tatttttaaa tgattttata ttatactaat actaaatata tttgttttag
     4561 ggaggtaatt tatgaggatt agtaaaaaag tattggcgtt agggatttca gcattattgt
     4621 taagtgttag ttttccaaca tcaataaacg cactagataa gattgaaaat attcaaggag
     4681 tagataaata tgaaacagct gggcttattt cagataagca agattttaca acagcagttc
     4741 ttatcaatgc agattctaca atggtagatg ggattgctgc aagtggtctt gcgggagtaa
     4801 ataatgcagc tattttatta actaataagg atgatatacc agaagcaaca cttcaaagac
     4861 tgaatagagt tactaagata tatgtaatag gtggagaaaa ttctataagt aaagatgtag
     4921 aaaaaatgtt acttatgaga agaatgcaag ttgtaagaat tgatggtgtg gatagagtag
     4981 atacaagtta taaaattgca ggtgaaatag aaaaaattaa aaacagtgat aagatgtttt
     5041 tggtaaatgg atttaaggat gaagctgatg cagtaagtgt tgcatctgtt gcttatagag
     5101 atggagctcc tataatattg acaaaagata taccttcagc agaagaagac acagacttag
     5161 acccatggtt tggagtttct cctgttccag tatatgctat tggaggaaag tctacattaa
     5221 gtgattacat agtgagtaga tatagagcaa ctagaattgg tggtgttgat agatatcaaa
     5281 ctaataaaaa tgttattgaa aagttctata atggagcaaa agagttctat atagcaagtg
     5341 gagacgattt agtttatgct ttagttgcat caccacttgc aaaaaatgct cctgttgctt
     5401 tagtttcaaa taaaagtgat aagagtatat taagtggagc taacaaagta acggcaatag
     5461 gtataagtga taatagtgtt atagagcagt gtttgaatgc tgtaaaaaag taatagagtg
     5521 ttattagtaa aatgaatata tacattgaaa attagtagat tgttcatttt ataatggtat
     5581 aaaattatga taagaggcta tcaaatggag ataagcctct ttttcattgt tgaaattatg
     5641 aaaatatatt ttattttata taaaaacagc gtaacttttt gtgtatcctc tcataaatgc
     5701 tctcctgcac aggatagcta caggaagtaa ggaacaggaa ataacggtag ccgtcaatct
     5761 ctcaggtaat catcagcagg gttgttatct gtaatacaac gaatggaaag aaaaggtatc
     5821 ttattcacat aacatacatg agtatacttg ctgtttccat atctactgtt aagggtgcaa
     5881 atttttcatt tattttttta cgtcccttat cagtaataaa agtttcacct gtagcaattc
     5941 ttccccaaaa tacggtttct tcttggtttg tttcttttac agccatttca cgtattttta
     6001 tcaattcacg gtttgcttta aaaaatacgg ttttcagcac tttagtttcc ccaaaacggg
     6061 gatttattgc tttcgtttgc tttttcgatt tttccagaca ttgtataaaa tatctttaag
     6121 aactaacatt gcttccagtt cattatattg gtattcctgt tttaattctt ccaacatcat
     6181 gttaacctta taagcataat taggaatatt aacatctgtc tgatatctaa gaagaatagg
     6241 gtattttttt ccccaggcat ttgtccaatt gattttttct tcggtttcct cacttgtttt
     6301 attaatcgct tcctgaattt cttctatatt aatatcaaaa tcaatcaatt catctaatga
     6361 cacattgtaa agtattgcta attttttcga ctgacgaatg tctgggactg tttcatccgt
     6421 ttcccatttt gaaacagtct gcctgcttac ccccagcttt tcagctacat cttcctgtga
     6481 aagcccatgt ttcttccttg cctgaaataa actatttcct aaattcattg aaattcctcc
     6541 ttatctgttt atgggattat tatattattt tataagaaca aactctacca actaaaatta
     6601 tcatatttgc tatttttctt aacataattc aaagactgta tttattctgt aaattaaaat
     6661 ctgctaacct ctgcgaatat caataagacg gaaaatatca aaatacattt tttctttatc
     6721 ttccaaatca atcattaact acttttatcc attccctgtt tttgtttggt acgtaaatat
     6781 atcttaattc tggtgtaaac tcctgtaaaa tcgcttcgac aacctctttc tctttctttc
     6841 caataacaac taaaacgtaa aatacttttc tttcagatat actgtgcata aattttttcc
     6901 agattttttt aatttgatat tccagccaaa ttcccaactg caagaactaa attctatttt
     6961 ttcattgcaa ttatatttcg atttcatatc cgagcaaaat tgagtaaaca caggattgtt
     7021 aacatattct ataacttcct ctaatgccag acaatatttt ttatcttgta tatcaatcat
     7081 atacgaactt ctttctccta gtggttaatg tttttttgta cattgtagca tattaccttt
     7141 atgccgtcat ttcttttctg tttttgaaaa aatacttatt aagcctatga tttgtaaagg
     7201 gtgttattca gcattactta cgcaacaagc ccttgacaaa ctatgtgata tagtatttat
     7261 gcggatattc tcgattttta aacgttttta ttatcaaaaa aagtatctca tgaactaaat
     7321 gtctattttg agacactatc tccctttctt atattatatt attttttata tggaataaat
     7381 ttcataagta ttatcttcct ttaatttttc taaccaaacc atctctaaaa tatctagttc
     7441 ttctaaatag tctttctctg ttttgttatc atcatgtttt ataagttcta aaacttcaaa
     7501 tgtaaatgct ttttcaccat attttttcca ttcattattt aattctttta tcatacatga
     7561 gccagcacca agctgaaatt tgagagagtt aaatttgctt tttatgtcat tactttttcc
     7621 aaggtaaatc tttttagtga tatcactttt tattatgaat acacctgtgt caaatttcat
     7681 ttctttgtat aattgtttta attcacgttt tctatccatg attttatata cctctcttta
     7741 taattgatat gtaatatgta attataaaaa tatattacat atcaattata aagagattaa
     7801 ttttatatgt aaatagtttt tataaattta attatgaatt agtcattaat aaacaagaaa
     7861 attagctatt taagtccatt ataaaggctg
//
LOCUS       DAEMAE010000172         7854 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEMAE010000172 DAEMAE010000000
VERSION     DAEMAE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.174"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..75
                     /locus_tag="KPH93_002869"
     tRNA            1..75
                     /locus_tag="KPH93_002869"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Thr,seq:tgt)
     gene            90..174
                     /locus_tag="KPH93_002870"
     tRNA            90..174
                     /locus_tag="KPH93_002870"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Tyr,seq:gta)
     gene            184..267
                     /locus_tag="KPH93_002871"
     tRNA            184..267
                     /locus_tag="KPH93_002871"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Leu,seq:tag)
     gene            292..366
                     /locus_tag="KPH93_002872"
     tRNA            292..366
                     /locus_tag="KPH93_002872"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Gly,seq:gcc)
     gene            391..467
                     /locus_tag="KPH93_002873"
     tRNA            391..467
                     /locus_tag="KPH93_002873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:425..427,aa:Arg,seq:tct)
     gene            477..552
                     /locus_tag="KPH93_002874"
     tRNA            477..552
                     /locus_tag="KPH93_002874"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Gln,seq:ttg)
     gene            561..636
                     /locus_tag="KPH93_002875"
     tRNA            561..636
                     /locus_tag="KPH93_002875"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:594..596,aa:Lys,seq:ttt)
     gene            639..727
                     /locus_tag="KPH93_002876"
     tRNA            639..727
                     /locus_tag="KPH93_002876"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:673..675,aa:Ser,seq:tga)
     gene            731..806
                     /locus_tag="KPH93_002877"
     tRNA            731..806
                     /locus_tag="KPH93_002877"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Phe,seq:gaa)
     gene            813..886
                     /locus_tag="KPH93_002878"
     tRNA            813..886
                     /locus_tag="KPH93_002878"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Met,seq:cat)
     gene            901..977
                     /locus_tag="KPH93_002879"
     tRNA            901..977
                     /locus_tag="KPH93_002879"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:935..937,aa:Ile,seq:cat)
     gene            995..1071
                     /locus_tag="KPH93_002880"
     tRNA            995..1071
                     /locus_tag="KPH93_002880"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1029..1031,aa:His,seq:gtg)
     gene            1080..1155
                     /locus_tag="KPH93_002881"
     tRNA            1080..1155
                     /locus_tag="KPH93_002881"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1113..1115,aa:Lys,seq:ttt)
     gene            1163..1236
                     /locus_tag="KPH93_002882"
     tRNA            1163..1236
                     /locus_tag="KPH93_002882"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1195..1197,aa:Cys,seq:gca)
     gene            1244..1320
                     /locus_tag="KPH93_002883"
     tRNA            1244..1320
                     /locus_tag="KPH93_002883"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1278..1280,aa:Arg,seq:acg)
     gene            1333..1408
                     /locus_tag="KPH93_002884"
     tRNA            1333..1408
                     /locus_tag="KPH93_002884"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1366..1368,aa:Val,seq:tac)
     gene            1484..1897
                     /locus_tag="KPH93_002885"
     CDS             1484..1897
                     /locus_tag="KPH93_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689662.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILVVAYADLGHLKLSDVVKRLSRQVD"
     gene            2206..5637
                     /locus_tag="KPH93_002886"
     CDS             2206..5637
                     /locus_tag="KPH93_002886"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG1689663.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5879..7807
                     /locus_tag="KPH93_002887"
     CDS             5879..7807
                     /locus_tag="KPH93_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG1689664.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3080 a         1009 c         1581 g         2184 t
ORIGIN      
        1 gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta ggttcgattc
       61 ctatcaccag ctccatgtaa aattaatacg gaggatttcc cgagcggcca aagggggcag
      121 actgtaaatc tgttgtcatc gacttcggtg gttcgaatcc accatcctcc accaaaaaaa
      181 tgcgcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc cattggcgtg
      241 ggggttcgac tcccttcatc cgcaccattt ttatttaatt ttaataaacg agcgggaata
      301 gttcagtggt agagcgcaac cttgccaagg ttgaagtcgc gagttcgaat ctcgtttccc
      361 gctccaaatt ttgtgtttat attgtaatgt gggtgcatag ctcagctgga tagagcaacg
      421 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg
      481 gattagccaa gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg
      541 catccccagc caaatagtat ggtctattag ctcagtcggt agagcacctg acttttaatc
      601 agggtgtccc gcgttcgagt cgcggataga tcaccaatgg agaggtgtcc gagtggttta
      661 aggagctggt cttgaaaacc agtgacttcg aaaggggccg tgggttcgaa tcccaccctc
      721 tccgccaaat gcccagatag ctcagtcggt agagcagggg actgaaaatc cccgtgtcgg
      781 tggttcgatt ccgcctctgg gcaccagaat atggcggtat agcttagttg gctagagcgt
      841 tcggttcata cccgaaaggt cataggttcg actcctatta ccgctactaa aatttaatgt
      901 gggccattag ctcagttggt tagagcgccc ggctcataac cggtaggtct ggggttcgag
      961 tccctgatgg cccaccacat taaattaact tatggtgggt atagctcagt tggttagagc
     1021 gccagattgt ggctctggag gtcgtgagtt cgactctcat tatccacccc aaattatatg
     1081 gtctattagc tcagtcggta gagcacctga cttttaatca gggtgtcccg cgttcgagtc
     1141 gcggatagat caccaaaaag gcggcgacat agccaagtgg taaggcagtg gactgcaact
     1201 ccttgatccc cagttcgaat ctgggtgtcg cctccaataa aatgcgctca tagctcaact
     1261 ggatagagtg tctgactacg aatcagaagg ttaggggttc gagtcccttt gggcgcacca
     1321 tataaatagc gagggattat agctcagctg ggagagcacc tgccttacaa gcagggggtc
     1381 acaggttcga gccctgttaa tcccaccagt gcaaacctct agtaaaacta ctagaggttt
     1441 tatttttgat tgaaaaagtt tttatattta ggaggtaaga aacttgtata tagtaatagg
     1501 aaatgaaata gttgatagtg aagaacttaa aataattata gataaaaata gtaaatttaa
     1561 agttgaaaaa gatttaagta aaagcacaaa aagagaagat gtaatagcat atcagttaag
     1621 tatagattta aattacttag attctcttat aaatgagcaa tgtaatttag ccagtctatc
     1681 tgatgaagag aaatttgatg aatatatgac tttatctgat gaacttgcac tggatttaga
     1741 agagctaatg ccaaaatata caataataaa tgctagagca tataaattag acgaggtaga
     1801 tggaattgta aaaataatac tagttgtagc gtatgcagat ttagggcact taaaactttc
     1861 tgatgtagta aagaggctat ctagacaagt agattaggat atgaatatga actatattaa
     1921 aaaaatgtat aaaatcttac gtaaagatga aaataattat gtaaacatag aaaaaaacag
     1981 tataaaatca agtcaattgt aggaattttc acataaattc atctgaaatt ctataaaaaa
     2041 tgccttaatt ttaatgaggt aaatcgagtt tacacagtct aaaaattact tatatttata
     2101 aatttatctt attaattaag gaactttctt aaacatcttc aaaaataaag tattttagtc
     2161 tataatcttt aatattacac aaacaataaa ggagaagaat agagtatgct taaaaagttt
     2221 aataaaatac tagtggctaa tagaggcgaa atagctataa gaatatttag agcctgttca
     2281 gaattgggga taaaaagtgt tggtatatac agtaaggaag ataaatatgg attgtttaga
     2341 acaaaagcag atgaatcata tttgatagga gaaggtaaag ggccaataga tgcatattta
     2401 gatatggatg gaataataga cctagcaaag agaaaaaaag ttgatgctat acatccagga
     2461 tatggttttc tagcggagaa tgcagaattt gctcgtaaat gtgaagagaa tggaattact
     2521 tttataggac catcatctaa ggtaatgaat atgatggggg ataagattaa ctctaaaaaa
     2581 atcgcaaaag aagttaatgt tcaaactata ccaggagtag aaaaagcaat aagaagtact
     2641 gaagaagcaa aggaagttgc aaacaaaata ggctatcctg ttatgataaa agcatctaat
     2701 ggtggtggcg gtagaggtat gagaattgta catcgcgaag aagacttaga gcttgaatat
     2761 gaaactgctt gtagtgaatc aagaaaagct tttggagaag atattatatt tatagaaaaa
     2821 tatatagctg atccaaaaca tatagaagtt caaatattag gagataacta tggaaatata
     2881 gttcacttat atgaaagaga ttgttctgtt caaagaagac atcaaaaaat aatagaatat
     2941 gcaccagctt tttcgttaga tgataaagta agaaaagaga tatgtgaaga tgcagttaag
     3001 ttatcaaaac atgtgggtta ttctaatgca ggaacattag aatttttagt agatgcaaat
     3061 ggtgggcatt attttataga gatgaacaca agagtacagg ttgaacatac tgtaacagag
     3121 atggttacag gaatagatat agtgcaaagt caaatactaa tagcccaagg ttacagttta
     3181 gatagtgaag aaattaatat aaaatcacaa gatgatgtgg aaataagagg ttactctatc
     3241 caatgtagaa taactacaga agaccctaag aataaattca tgcctgatac aggtaaaatt
     3301 caagtttata gaactggttc aggatttggt ataagactag acggaggaaa tggatttaca
     3361 ggggcaaaca taagtcctca ctatgatagt ttattggtta agacaatatc ttgggataga
     3421 acattccaag gagcaataaa taaaactata agatctataa aagaacttag agttagaggt
     3481 gttaaaacta atgtaggatt cctagttaat gttttaaata atcctatatt ctcaaatggt
     3541 aaatgtagta ctaagtttat agatgaaaat ccagatttat ttgaaataac agaaagtaaa
     3601 gacagaggaa caaaattact tcaatttata ggagatgtaa ttgtaaatga taatgcttgt
     3661 aaagaaaaac cattatttga tgcattacat gacccaagaa tggataagga tggctcaaaa
     3721 tcagaaggaa gtaaaatact tttcgataag ttaggtaaat ctgcatatat agaaaagata
     3781 aaaaatgata agaaattatt attaacagat acaacaatga gagatgcaca tcaatcatta
     3841 ttagcaacaa gaataagaac atatgactta ttaaaagctg ctaagccaac agaaaaatat
     3901 caaaaggatt tattctcact tgaaatgtgg ggaggagcta cttatgatgt tgcatataga
     3961 ttcttaaaag aatctccttg gcgtagatta caaaagctaa gagaagaaat accaagcata
     4021 atgttccaaa tgttgcttag agcatcaaat ggagttggat ataaaaacta tccagataat
     4081 gtaatagaag aatttacaaa agaatctgct agacaaggta tagatgtatt tagaatattt
     4141 gattcattaa actgggttga aaatatgaag ccatctatta atacagcttt agaaactgga
     4201 aaaattgttg aagcaactat gtgttataca ggtgatatac tagataagac taaaactaaa
     4261 tacaatctag aatattatat taaaatggca caagaattag aaagcttggg agcagatata
     4321 atagcaataa aagatatgtc aggtcttctt aaaccatatt cagcttacac actagttaaa
     4381 gagttaaaga aaaatgtaaa agcaccaata catttacaca ctcatgatac aagtggaaat
     4441 ggtgtagcaa cttgtttaat ggcatcagaa gctggagtag acataattga tgctgcatta
     4501 gaatctatgg caggacttac aagtcagcca tcacttaatg ctatagtaga ggcacttaaa
     4561 aatacagaga gagatacagg tatagattta tttggatatg atgagttagg aaaatattat
     4621 aaggatttaa gaaaagttta taataaattt gaaagtgatt taactaattc ttgtgcagaa
     4681 atatataatt ttgaaatacc aggaggccaa tatacaaact taaaacctca agctgatagt
     4741 ttaggattag ttaatagatt tgatgaagtt aaagaaaaat ataaagaagc taatgaagtt
     4801 gtaggagata taataaaagt aactccatct tcaaaagttg taggagattt agctatcttt
     4861 atgactaaaa ataaattaga taaagacaat ataatagaag aaggtaaaaa tttatcattc
     4921 ccagattctg ttgtggatta ctgtaaaggt atgattggtc aaccagaagg tggaatacca
     4981 aaagatttac aagaggtagt tctaaaaggt gaagaagcta taacagtaag accaggttca
     5041 ttattaccag cagaagattt tgatgaaata gctaaatatc taaatgaaaa atatgatata
     5101 aatgctaata taagaaatgt tataagttat gctttatatc caaaagtata tgaagattat
     5161 atcaaacatt tacaacatta caatgatata tctaaacttg aaagtgatgt attcttctat
     5221 ggattaaata aaaatgaaga atgtgaagtg gaaatagaag aaggtaaggt attaacaata
     5281 agattagttg aaatcggtga agttaaggaa aatggattta gaactatagg atttgagtta
     5341 aatggaatgg ttagagaagt agaaataaaa gataagaatt tctctggaaa aattaataat
     5401 gttgaaaaag ctgatatgaa cgaccctctt caaattggag caagcatacc aggaaaagtt
     5461 ataaaaataa tggttaaaga agaagatgaa gtaaaagcga atcaaccatt aatcgtcata
     5521 gaggctatga agatggaaac tataatagtt gctaagactg atggagtaat taagtcaatc
     5581 aaagtaaaag aagatgatat ggttgaagat aaacaattat taatgataat gaagtaattg
     5641 aattaaaata aattaaaaag cactctgaaa tgtttaataa aaaatgtttt agagtgtttt
     5701 tatttatttt aattaaatat ttgttaaaaa aaagaggaca atcacctcca gatgtagaaa
     5761 taaagctagt aaggaaaatg ttatctaaaa attctaaata tagtaaatat tttccataat
     5821 atttcttagg aaataacagc aaagataaaa tacataaatt taaaaggtgg gtgcttatat
     5881 gaaggtttat gatagtaaaa tgttgagaaa tgtagcggta ttaggacata gcggatgtgg
     5941 aaaaacaaat ctaatcgaaa ctatagctta cacagcaaac actaataaga tacctaaatt
     6001 aacagacaag gtaaatatga cttatagcat gggattaata cctatagagt ataatgatta
     6061 taagtttaac ttattggata ccccaggata ttttgacttt agcggagacg ttgtgtcatc
     6121 tcttagagca agtgatgcag caataatagt aatagatgca acagcgccta ttcaagttgg
     6181 tacagagaaa tcattagaac taactgagag tattcctaag ataatgttta taaataaaat
     6241 agataatgaa aaggcaagat acaaagatgc aatagcaatg ttaagagaaa aatacaataa
     6301 taagatagtt cctatgataa gtcctatata caaagataaa aactttgtaa agttacataa
     6361 tgtttttgaa aacatagatg atttagaagg tgaatttaag gaacaagcta tgagtgttaa
     6421 agaagcatta atggaactta tagcagaaac tgatgaccaa atattagata aatattttaa
     6481 tggagaagaa ctaactacgg aagagataca aaaaggtatt attataggta tacaaagagg
     6541 tgatattata cctgtaatat gtggttcaac tataaataat ataggcacaa aagaaatatt
     6601 agatacaata tcaagttatt tagaacctat ctttacagag gaaagcaaac catttagggg
     6661 attagtattt aaaactatgg ttgacccatt tgttggaaag atgtcatata taaaaattac
     6721 tgagggtgta ctttctaagg ataaagatgt ttttaatata aataaaaatg taaaagaaaa
     6781 aatagctaat atatatacat tgagaaatag tgaattagta gaaatagaaa aagctaaagc
     6841 tggtgatata gtagttataa ctaaagttaa ttctctaaaa acaggagaca ctatatctgc
     6901 agataaagat gcagaagctt tagaaaagat agattttcct aaaccacaaa tatattatgc
     6961 agtaactcct aagaataaag gtgatgaaga aaaagtagca tctgtattaa ataaactagt
     7021 agaagaggac cctacattac attggtatag aaatacagag acaaaacaag cacttttagg
     7081 aggtcaagga gaacttcata taaaaacaat taaaaataaa atgaaagata agtttggtgt
     7141 tgatgttgaa ttgaatgacc ttaaagttcc ttatagagaa actataaaag gaacagctga
     7201 tgtacaagga aaacataaaa agcaatcagg tggtcatggt caatatggag acgtaaaaat
     7261 aagatttgaa agatgtgaat ctgattttga atttacagaa gaaatatttg gtggttctgt
     7321 accaaaacaa tatataccag cagttgaaaa aggtctaaaa gactctatgc aaaaaggtat
     7381 attagcaggt tatcctgtta caaacataaa agctacgctt tatgatggtt cataccatga
     7441 tgtagactct tctgaaatgg cctttaagat ggcagcaagt gcagccttta aaaaaggcat
     7501 ggaagaagcg cacccaatac tactagaacc tataatgaaa cttaagataa ctgttccaga
     7561 agaatatatg ggagatgtta tgggtgatat aaataagaga agaggaaaaa tatttggaat
     7621 ggaaccagat gacaaaggga aacaaataat atttgcagaa gctccacagg cagagacatt
     7681 taaatatgca atagatttaa gagcaatgac acaaggtaga ggatactttg aaatggaatt
     7741 agagagatat ggagaggttc catcacagtt tgcagaaaaa ataattgggt tagctactgc
     7801 aaagtaatga acaaaataaa tagaatcata aataattaat agatactatt aaaa
//
LOCUS       DAEMAE010000173         7821 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEMAE010000173 DAEMAE010000000
VERSION     DAEMAE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.175"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    96..307
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            470..1759
                     /locus_tag="KPH93_002888"
     CDS             470..1759
                     /locus_tag="KPH93_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG1689665.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1813..2892
                     /locus_tag="KPH93_002889"
     CDS             1813..2892
                     /locus_tag="KPH93_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG1689666.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     SKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGNTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2913..4457
                     /locus_tag="KPH93_002890"
     CDS             2913..4457
                     /locus_tag="KPH93_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG1689667.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKDKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALR
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCDEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4634..5176)
                     /locus_tag="KPH93_002891"
     CDS             complement(4634..5176)
                     /locus_tag="KPH93_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG1689668.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYLEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMLPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5324..5608)
                     /locus_tag="KPH93_002892"
     CDS             complement(5324..5608)
                     /locus_tag="KPH93_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1689669.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYVL
                     VYRENEDKEKNEHDKNLNEIINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5671..6435)
                     /locus_tag="KPH93_002893"
     CDS             complement(5671..6435)
                     /locus_tag="KPH93_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG1689670.1"
                     /translation="MNNYLKFATYQLIGFTSSIPKLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAEASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6717..6983)
                     /locus_tag="KPH93_002894"
     CDS             complement(6717..6983)
                     /locus_tag="KPH93_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1689671.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7025..7780)
                     /locus_tag="KPH93_002895"
     CDS             complement(7025..7780)
                     /locus_tag="KPH93_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689672.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEANSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEVQNNKIPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2724 a         1004 c         1278 g         2815 t
ORIGIN      
        1 taaaactaaa aaatgcaagt aattgacttt ttaagttgac aaaatattgc atatatatta
       61 tgatataaat cataaattaa ctacaaatca atattatata ttcgtagaag aagaaagtag
      121 cataaaggaa ttttacagag agttagattt ggctgagaac taatattgga atttatgtga
      181 aaaggacttt ggagaatact attgtaatta tgttagaata gtggtttaat tcgccttaag
      241 aaaatgagtg agtaaaatta atttttacta ataagagtgg taacacggat ttatattcgt
      301 ctcttggatt tttccaagag gcgtttttta ttataaaatt atttcagaaa atttaaaact
      361 atatattaaa tgaaaattat aaaaatatga tagttaataa atttataaat tagaaaattg
      421 taaaatatac taaggtcagt agctttacga tttaaatgga gggatgaaaa tggaagaaga
      481 aaaaattaac tatgctctgg aacagattcc agagaatcaa aaacgtggtt gggtagctat
      541 gttttcagtt ttagtagcaa taggtgtgga tttatcatct gttattttag gcgctgagtt
      601 ggcacagagt atgccaatga aacaagcaat attgtcagta attgttggtt catttttttc
      661 tgcaattttg tatactacat gctctttggt tggttcatct acaagtttat ctacatctat
      721 gataacaaag tatgtatttg gagaagctgg agcaaaaata ttttctctag ttataggggt
      781 ttctctatta gggtggtttg gagttcaagt aggtttcttt gctcaaaatg cacagataat
      841 aataaaagat atatttaatt tagatgtcag tatgcagatt ttaagcctaa taggtggcct
      901 cttgatgatg agtactgcaa tttatggata taaggcaatg gagaagttga gtgtgtattc
      961 agtaccattt ttattagttc ttatgatgct aactatattc ttagctttta gagcaaatgg
     1021 aatttcagtt gatgataata tgaaatctac tatgacattt gcaggtggag tttctctgtc
     1081 aatgagtata ataatagttg gtgcaattgt atctcctgat ataagtcgtt gggctaaaag
     1141 tagaagagac tgtgcgcttt caagcttttt gggaatacaa tttggaaatg cttttatgat
     1201 aatagtttca atagtattag taaaatgtat gggaacttca gatattatga gaatttttat
     1261 tacattagga attgctatac cagggataat agtacttaca ttagctcaat ggactacaaa
     1321 tacaagtaat gtatattcag catcattaag tatagctctt gtacttaaga aggctcctga
     1381 gaaagtatta actatagtcc ttggtattat agcaacatta ttggctgtat ttggaatata
     1441 tgaaggtttt ataggatttt taaatttact tggaattgta attgcacctg ttggtggtgt
     1501 atatacagca gaatattaca tagtaaagca agaacttaaa ggctttgata aaggagtatt
     1561 atataaacca atagttaaac gttcaatagt gtcttggata attggtatat taataactta
     1621 cctttcaaca tatggattta taactcttac tactatagca cctttagatg gttttatagc
     1681 agggtttgta gtacaatcta ttattgggaa agtgctttgt tcaacgaaaa ataagcaaga
     1741 aatagacaaa gcagtataaa gaaatttgtt tataaagtat aaaataaatt agaaatatga
     1801 ggagaaagta acatgagatt tttaacagaa gaaagtgtag ataaaatagc agttggtgca
     1861 gcagtgcttg gaactggagg tggaggtgac ccatatgtag gtaaattggt tgcaaaacaa
     1921 gccattaaaa agtatggtcc tgtaaaagta ataagtttag atgaacttga tgatgatgct
     1981 ttggtagttc cagtatctgg tatgggttct cctgtaatta caatagaaaa attactttca
     2041 gaagtagagt taactacccc tttagaaata atggagaaat tgttaaatag aaaagtggat
     2101 gttataattc ctatagaaat aggagggata aactcactta tgccgatagc agttgctgct
     2161 aaaaaaggac ttcctatctt agatgcagat tctatgggta gagctttccc tgaagctcaa
     2221 atggtaactt tttatcttga aggatacgag gcatctcctg ccgtaatgtc tgatgaaaaa
     2281 ggtaattctg ctatccttta tcctgtggat ggaatttggt cagaaagatt agctagaact
     2341 ttaactgtag aaatgggagg aagttcttct attagtgact ataatttaag tggagctcaa
     2401 gttaaaaaag ctgctatagg caatactctt acaatagctg aaactatagg aggattttta
     2461 cttgaaagta aatctgattc aaaaggtgct gttgagaata tactaaaaga attacgtgga
     2521 tacaagttat ttgaaggaaa agtaattgat attaagagag agttgaaagg tggatttact
     2581 agaggacatg ccttttttgc tgggattaat gagtatgatg gagagtattc tatattattc
     2641 caaaatgaga acttaattgc caaaaaaggt aatacaccac tttgtattac accagattta
     2701 atagcagtat tagatttaga aactggattt ccaataacta cagaaagaat aaaatatggc
     2761 tctcgtgtta tggtagtagc ttttccttgt aatgaaaagt ggagaacaga aaaaggaatt
     2821 gagacagttg gacctggata ctttggatat gatgtggaat ataaaacagt agaagaatta
     2881 caaggaaaat aaaagtttag gaggaaattt tgatgtatcg tgtaggaatt gatgttggag
     2941 gaacaaatac agatgcttgt atactggatg gggaattaaa agtaattcat tcagttaagg
     3001 tagcaactac aaaagatgtt gaaactggtg tttacaatgc cttaaaaaaa gttatagatg
     3061 aaagtaaaat tgatcattca ttaataaaat atgctatgct tggtacaact cattgtacaa
     3121 atgctattgt agaaagaaaa agacttaata atgtggctat gataagaata ggaaagccag
     3181 ccactactgc cattcttcca tttatagatt ggccagaaga cttaagagaa aaaatagaat
     3241 tagattcaat attggtatct ggtggatatg agtttgatgg tagaaagata aatgaacttt
     3301 gtaaagatga agttgtagag ttttgcaata gaataaaaga caaagtggaa agtgttgcaa
     3361 taagtggagt atttgctcca gtaaataaac aacaagaaga agaagttgca aaatgggtta
     3421 gagaaatttt aggggatatt cctatatctt tatcaaatga aataggtaat ataggtatat
     3481 tagaaagaga aaatggagct attttaaatt ctgcattgtc tcaagtaggt aaagctgtat
     3541 ctttaggatt tgaaaaagca cttagagact taggtataga tgctcaaata tatttcagtc
     3601 aaaatgatgg aacattaatg aatttagagt atacaatgaa atacccaatt tttacaattg
     3661 gatgtggaat aacaaattct ataagaggag cttccttctt atcaaaagta aaaaatgcaa
     3721 tagtactaga tgttggtgga actacaagcg acttgggtgt actatataat ggattcccta
     3781 gagaatcatc tattccagtt acaattggag gagtacatac aaactttaga atgccagatg
     3841 ttttaactat tggtcttgct ggtggtacat gtgtaaaagg agaaaaagat aatataaaga
     3901 taggtcctga aagtgtggga tatagaataa ctgaagatgc aataatattt ggtggagaca
     3961 ctctaacttt atcagatgtt gttacaaaat tagatatggc atttgaagat agaaaaagca
     4021 atgttgaaaa cttagataaa gagtactgtg atgaggtata taagaaggtc attttaaaat
     4081 tagaggatgc tgtagaccaa atgaagacta gtcaagatga tgttgaattg gttttaactg
     4141 gtggaggaag tataattgtt cctgaaaaac taaaaggaat aagcaatgtt ataagacctc
     4201 ctcattatac tgctgcaaat gctattggtg cagctttagg acaaataagt ggagatgtag
     4261 aaaaagtata cagtttagat aaaatgtcga gagaagatgc catagaagat gcaaaaaaag
     4321 aggctgtaaa taaagctcta aaagctggtg ctaaagaaga tactatagaa gtattaaata
     4381 tagaagatgt tccacttgca taccttcctg gtaatgcact tcttataaaa gtaaaagttg
     4441 ttggtgattt aatttaaaga ataaatataa gtatttccag attcgtattt ccagaatcat
     4501 ataaatattt ctggactcat ttttaaactt attttatcaa taaaaacagt ataacttttg
     4561 actatattct tataagtttc cgaacagccg atatatacct attttttgag gtttatcggc
     4621 tgtttgttat cttttatcgc attccaacta tttttcttac cgtatttgct gtacgaattg
     4681 taatcttatc acttacattt gaactggcag tctttgacca ccaatttgtc tttttataat
     4741 ctttacgact aaaagaccaa aagatagcat ttttataatg atatactttt tcatattctt
     4801 ctttaggatt tccaacctca tcataaaact cgtcataaga taagtcagga agcataaaaa
     4861 taagattatc atatatttct ttgttgttat ctccccacca attaggagca ttatttagta
     4921 tatcttttaa ctcctcttgt gaaataataa aaactggtat ttccaattca aactgttttt
     4981 ttatcattaa tcttattgta tttgagataa ccttgctgtc atctacatca cttaaaaaga
     5041 caacattacc actattaagg tatgtagaaa cttctaaata accaagttct acaaagcttg
     5101 cttttaattc agacataatt atcttgttct taccacttat attgatacct cttaataatg
     5161 cgatataact tttcatataa taatttcacc tctaaacatt cacatttttc tatgtagtac
     5221 aaactggaat ttgcatcttt tataattctt tcaaaacttc ttcatatgta atttttttat
     5281 gctaaaaaaa tcggtactta atttgtaccg atttttgttt ttcttagtaa agaactcgtt
     5341 tttttgcttc tgctatagct ctagcagcac tttttgcttg cattcttttt tcttcttctt
     5401 ttttaaattc atttattatt tcatttagat tcttatcatg ttcatttttt tctttatctt
     5461 cgttttctct gtatactaat acataatcta ttattgattc gtttattatc tgggatatac
     5521 ttttattttc attaattcct attctcttaa cttccttaag tagagtctca tcaatatgaa
     5581 ccgttgtttg ctttgtattt tttgccacct taatcacctc ttaaaaaaaa ttctaacatt
     5641 aaaatttttt ttagtcaata aattttaata ttaaaattct ttaaatgttt tatttgattt
     5701 tctaaaattt tctattgtat ttatcatctt attttttata ttttcaccta gtacagaaga
     5761 ctttaatcta ggattttcga tatggtcata aattggttct aaaaattcga cttcaacagg
     5821 aacagagtta attttaccaa taggttggta tccttcataa gtatcttttg aattttttat
     5881 tactagcgga acaatagggc attttgcttt atatgctatt tttaaagctc cacttctaaa
     5941 ctccgaaacc aatgaatttg gttctttaat ccatgttaaa tctccttctg gaaatactgc
     6001 catactttga cctgtaagta tattttgaga ggcttcggct atactcttta taccttcacg
     6061 attgtttttt ctgtttacat atacacatct taataatttt gcccattctt taaatatagg
     6121 tatatttctc caaactggtt catctgcaat tacacatcca attggtcttt ctacacttgc
     6181 tgttagtatg aagctgtcaa gcatactaga atgatttacc acaaatagaa gtggttcttt
     6241 tggcagtgtt tccttaccaa ttatattaag ttctatattc actatatcta aagatttttt
     6301 agcttgtttt tgcataaatt caaatttttc ctttaagcta aatttatcag gattcttttt
     6361 aattttaata agcttaggta tagaagatgt aaaacctata agttgatatg tagcaaattt
     6421 taagtaatta ttcataaaat tatctcctta aaataaaatt atttattact gtaaagattt
     6481 taacataaaa taaaatttaa gtctatttta attcaatttt gtaacatata tttcaaatag
     6541 acagagattg agattttata tttctaattt tagcaatcat attttatcaa taaattacta
     6601 ttctattaat ttaattaaat aacctttaaa tttaattata aaaactgttg tagaattttc
     6661 tacaacagtt taaaaaattt accttacaaa aacttatttt ataacaagag agtatattat
     6721 aaggctagta cagcccattt tttgcactga gaaattcttt tgccatcatc accttgttct
     6781 ttgtcatatg caaattgatt aagcatttca catggaaatg tatcacatag accacaatgt
     6841 gtaaggtttt tatcttcaca acaagatttt acaggacatt gttcacccca aaatggtttc
     6901 tcaatctctg tacaaccttt acaattgact ttttccttgt acccacattc ataacataaa
     6961 attccgcatc ttgattctat cattctaagt cactccttaa atataaattt tatatgattg
     7021 tatatcaaaa tttatacttt tcattgtaaa aatcggacaa attttttata taggttgttg
     7081 gattaacacc acaaatagat ttaaaatcat ttataaaatg agattggtcg taataaccaa
     7141 tatcttgtgc taaagtcgca aatggtattt tattattttg tacttcttgt aatacaaggt
     7201 ttatacgaag taatctaagg tatgatttaa cactcatccc aagagaatta ttaaaaattc
     7261 tatttaaatg tcgttcacta taacaactaa tttgtgaaat gtttttaaca gacataatag
     7321 aattctgttt tctagcattt tcaagaattg gtatagtcat atctactata tttgatttca
     7381 ataagtatga taagaataac atatctaatt gttctactaa ttcctttata gtacttgttc
     7441 tttcaaaaat ggaattagcc tctaagctaa ataatgtgtt aaaatcttca agtgaaattt
     7501 ttaaatctgt agattctctt tgagaaagac ctgtcaaata atatactcca cctgggcgaa
     7561 actcaacgaa tactctaaaa agtacatttt ctatatcatt ttttacaata gtagtttttg
     7621 ttgtagctcc ccaaaatgca ctttctatac cttttttatc aaatttaaat atcatacatc
     7681 cactagcatc aggtattaat gtaagatttt ctttaatact tgtgtctgga atagatattg
     7741 tatagtgtgc tatatactgt cttaacaatt tatgtggcaa aatatatgta tactgtttgc
     7801 aggtatttaa aatgttattg a
//
LOCUS       DAEMAE010000174         7755 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEMAE010000174 DAEMAE010000000
VERSION     DAEMAE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.176"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..2333
                     /locus_tag="KPH93_002896"
     CDS             105..2333
                     /locus_tag="KPH93_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG1689673.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLKPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSGDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSVSALNNVLSLSNGIGRINLNILNNFDSKISRPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2570..2869)
                     /locus_tag="KPH93_002897"
     CDS             complement(2570..2869)
                     /locus_tag="KPH93_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG1689674.1"
                     /translation="MYKNINKNQLKEMMKNEKNILLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3077..6118
                     /locus_tag="KPH93_002898"
     CDS             3077..6118
                     /locus_tag="KPH93_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG1689675.1"
                     /translation="MFKQKLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNSESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDEGKINLDTPVVNYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLGDNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRNGKGYYLVDEKYTSQSYMFGSVKATLGLSD
                     ETPGYIVNTKIIDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGIPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWGTAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKDIA"
     gene            complement(6280..7554)
                     /locus_tag="KPH93_002899"
     CDS             complement(6280..7554)
                     /locus_tag="KPH93_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1689676.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NENEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCINACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNDALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2865 a         1085 c         1111 g         2694 t
ORIGIN      
        1 ttaaaattaa gacaaaattt taactttact atttgtgtaa aaagtaatat aatttaaaat
       61 tgattataaa tctaactttt ttctttatca aaatatttta ggaggtgaga catttgaaaa
      121 aaactaatag tttaaaagaa attttaaact ggaataatat caagaattta aaaccttcaa
      181 agggtttaaa aaatatatta gaaattttta aaagtgacct aaaagatata cgtggtaatt
      241 atgcagtact tgttatagtt ttaggtcttg cttttttacc atctctgtat gcttggttca
      301 atataaaggc atcctgggac ccttatggaa gcactgccaa tatgcttgta gcagtaacaa
      361 atgaagataa aggaaatgaa atagcagggc aaaagattaa tgtaggagac caattaattg
      421 aacaactcaa agataacaag agtttaggtt ggcaatttgt agatgaaaaa acagctcttg
      481 aaggtgttaa aaaaggtact tattatgcat cactaaagat tccaaaaaat tttacaaaag
      541 atttgacatc tttaattaca gatgatgtta aaaaaggcga aattatctat actgttaatg
      601 aaaaaataaa tgcagttgca ccaaagatta cagataaggg tgcttctaca atacaactac
      661 aggttaatca gactatagta aaaacagtta gtgaaatcgt tcttggtata tcaaacggag
      721 ttggtgcagg aattgaacac agtcttccaa aattgacaaa agttgaaaat tcccttattg
      781 accttcaaaa gagatttgac catataaata agactgttga tttagcttct gatgctactt
      841 caaaattatc tgatacagtt aaagatatta aatctgattt acctacaatt aaaaaaactc
      901 tgaatgatac aaaactgtta tcaggtgact taaaaaaatt cttagaggat acaaatgata
      961 atttagatga actttctcct cttattaagt ctgacttaaa cttaatggtt gacctctcat
     1021 cttctgcttc ttctcttaca ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg
     1081 tacctaagtt gatagataat ttatcagaaa aactttctaa tttgcaatct ttaaacgata
     1141 ctttggtgga ttttttaact aaattaaacc aacttacatc aaataataga ttagatgatg
     1201 taatagataa tcttgaagac tctagcaata aaatagattc atctatatca acactaaatg
     1261 atattaaaaa caaagttata tctggacaac aaccatctgt aagtgctttg aataatgttt
     1321 taagtctatc aaatggtatt ggaagaatta atttaaatat tttaaataat tttgactcaa
     1381 aaatatcaag acctataaat agtatatttg caaacagtat aaaagtagca aatgatataa
     1441 ttactgtttt agataaggca gaagcaaaac ttccaaaggt tgaagaaata cttactactt
     1501 ctctaaaact ttcaggaaat gcacaggaaa gtatatcatt gataagagaa agacttcctt
     1561 tagcaaaagg gatgttggat gatttgattg atactttaag caagattagt aatggtgaag
     1621 atatgaaaaa attagtcagt ttgcttgaaa gtgatatatt aacaaagtct aatttcttaa
     1681 aagaacctgt tgaggtgaaa acagagaaat tatatccaat tgaaaattat ggttctgcaa
     1741 tgacaccatt ttatagtgtt ctttctacat gggttggtat attacttatt tcagcactat
     1801 tgtctacatc tgtacatgga aactataaac cttatgaaat ttactttgga agaggcctta
     1861 cttttttaag tatagctctt gttcaaggtt ttataatcgg aactggagat atattattat
     1921 taggagtaac agcaaaacat ccaattttat tgatagttct gcttatgttt actagtatgg
     1981 tatttaactt tatagtgtat tctttagttt ctgtatttgg aaatgtagga aaagcaattg
     2041 caatagtact attagtcgtt cagattggtt cttctggtgg tacattccct atacaagtta
     2101 cacctacatt cttccaaaga ataaacccta taatgccatt tacctatgca ataggtgcta
     2161 caagggaagc agttggtggt atatatgctc caaatctaac taaagatatt ttagttctat
     2221 tactatttat gattctgtct atttgtttaa atgtacttct aaaaggacct ataaatagag
     2281 ttataaaacc atttaatgat aagtttggcg atagtaattt aactggtcat taaaaaatta
     2341 attctgtatt taaatatatt ttttaataat attctaagtc catataaaaa ggtagtagct
     2401 aaaaagctac taccttttta tatcttaaaa actaatactt actacaaaaa ctgtcataga
     2461 aaaaagcgaa aaaattcgtt ctttcctaaa tttatcttat aaatatttag atatattcct
     2521 acttaataag cagagctata catagtattt aatgtataac ttacattatt taactcatat
     2581 aatcaattat tcctgagttt aaattatata aatgctcaaa accttcttca gctaacaaat
     2641 tacatgcagt aacacttcta tgaccacttc tacaataaac tactactttc ttatctttat
     2701 aatcgtaaat ttcatctata ttattgatta gttcttgtaa ggatatattt atagcatttt
     2761 ctattttata ttccttaaat tcatctttag ttcttacatc caataataaa atattctttt
     2821 catttttcat catttcttta agttgattct tatttatatt cttgtacata tgtctaaaat
     2881 tcctccattt ttatttatat attacctata ccctttattc tttatacaac aacaaatatt
     2941 atttttatat ttttctctgt tatataaaaa gctatactta taaaagcttt ttttattgat
     3001 aataatctgt tgattaaaat aattaagaat gttatgctaa ttataaatta ttaaaaaggg
     3061 aaagaggtaa tgtaatatgt ttaagcaaaa gctctctaaa ttattatcta gtacattagt
     3121 attgagtatg ctatttactg ctgctccaaa tattactttt gctgataata caaaagataa
     3181 ttccgaaaaa tatcaaagtt ctgatataga attacatgat tatagtaaaa attcggaatc
     3241 ttacacaaaa actaaagctc ttgccaaaga gaaaatccaa actttgctat caaagtatgg
     3301 tgctgtaagt gctcaatatg ccttgattga taatggtaaa attgagattt ctggtaatgg
     3361 tggagtttac agcaaacaag ataacaaaaa cttaaataaa gataacatgt atagtatcgc
     3421 atctatcagt aaaatgttta ctacaacagc tgttatgaag ctggttgatg agggaaaaat
     3481 aaatttagat actcctgttg taaattatat acctgaattt aaaatggctg atgatagata
     3541 caaagaaatc actccaagaa tgttattaaa tcattcttca ggattgatgg gaagtagttt
     3601 taaaaatact attttattag gagacaatga ttcttatggc catgataatt tcttaaaaga
     3661 attacaaaaa caaagattaa aagctaaacc aggagctttt tctgtttatt gtaatgatgg
     3721 atttacttta gctgaaattt tagtagagag agtatctggt atgtcattta ctaattttct
     3781 tgataaatat ataaataatc cacttaactt acaaaacaca aaaactccag aaaatagttt
     3841 tgacagttca aaactagcaa aagcatatgt accttattgg gaagatgcag ttccacaaga
     3901 taaccttaat gctataggtg caggtggttt atattcaagt gcagaaaacc tatgtacatt
     3961 tgcacaaaca tttatgaaaa actcaaatgg tattctttct cctgcttcag taaaagctat
     4021 ggaaaacaaa gaatatttaa atggtctatg gcctgaagga gaagactcta tacttggata
     4081 tggtttaggg tgggattgtg tcaacactta tccatttaat cagtataatt taaaagctct
     4141 tacaaaaggt ggagactctt tattattcca cagtaatctt atagttcttc cagatgaaaa
     4201 tatggcagtt gcagttcttt cttctggtgg aagtagtcaa cttaacgaaa taattggaca
     4261 agaaatctta ctatctgcat taaaagaaaa aggtaaaatt aaagaaataa aacctgataa
     4321 aacatttagt aagccacaac aagttaaaat gccaagctct ttaaaagaaa atagtggtct
     4381 ttatgcttct tcaaatatga taaaagttga tgttaatgac aatggtactt taacagtatc
     4441 ttctccttat atagaaaatg gacctgaaga taaatacgta tacataggtc aagatagatt
     4501 tgtatctgaa aaaggtaata gttgtttaaa atttgtaaaa gaaaaaaaca atatcacata
     4561 tttaaatatg agctcttatg atgatgtccc tggattaggt cagacagcaa gtttatacta
     4621 tgtagctcaa aaggtagatg acaacaatat atcaaatagt gtaaaagaag tgtggaaaaa
     4681 aagaaatggc aaaggttact atctagtgga tgaaaaatat acttctcaaa gctatatgtt
     4741 tggttctgtg aaggcaactc ttggtctttc tgatgaaact ccaggttata tagttaacac
     4801 taagataata gatgagaata actcaaatgc ttttatagaa atacctggtg taataggtcg
     4861 tgatttatcc gacattaaac ttcataaaga aaatggtaca gagtatttat cttttggcac
     4921 actaacgtat gttagtgaag attctataac taatttgcca gcagaaaaat cttttacttg
     4981 tgaactagaa tccaatggtt atgcaaaatg gtataagatt ggtgacgata tagcaaataa
     5041 aaaaatagaa gttaatcttc ctcaaaattc atcttttgct gtttatgatg ataaaggtat
     5101 tcctgtaaac tattctttag ttacaaagaa caatagagtt agattgccaa aaggaggcgt
     5161 aattgttttc ttaggaagtc caaatgctag atttgaagtt acatatcaag atgaggtaaa
     5221 tgcaagtgca cttacaggaa ctgatagata tgaaacttct attaagataa gtcaagcagg
     5281 ttggggaact gctgaaaatg cagttttaat aaacgactca gcaattgcag atgctttggc
     5341 tgcaacacca tttgcataca agaaaaacgc acctatatta ttgactggaa gctctcaaat
     5401 aaatgaaaaa actttggctg aattaaagag attaaaagtt aaaaatgtat atgtagttgg
     5461 tggagaggct tctataaatg aaaaatcttt agatactata aaatcaaaca atatctctgt
     5521 atctagaatt tcaggaagtg atagatatca aacttctatg aacattgcta aagaattgaa
     5581 taatattagc aatatatcaa agatttcagt agttaatggt gaaaaaggac tagccgatgc
     5641 agtaagcatt ggagctgtat cagctcaaaa tgatatgcct ataatactta ctaatgaaaa
     5701 tagtaatata actgaaataa ataacttgtt taaaaacaaa aaaattgata aatcttatgt
     5761 aatcggtgga gaatatacgg tttctaaaaa tatagaaagt aaacttcaaa atccacagag
     5821 aatatcaggc agcactagaa atgaaactaa tgcaaaagta ataaaagaat tttacaagga
     5881 ttctaagata gacaacttat atgttgctaa aaatggaatg aataaacaag atgatttaat
     5941 agatggatta tctgttggag ttttggctgg aaaaactaaa tctcctgtaa tgctagtagg
     6001 aaactcacta gattataatc aaaaagaact atttaaaact atgagattta aatctgtaac
     6061 acaaattggt ggaaatggaa atgaaaactc atttaaacaa ataaaagaca tagcttagca
     6121 taactaaaaa aggttatatt caagtaacat taattgaata taaccctttt ttatttcagt
     6181 tataaatttt atatgtgtaa agataaaatt tatatattac atgataagtt tattaaaata
     6241 taaataagtc taaaaacttt gcctttttta agttagtttc tatttgtcat tcatacttaa
     6301 caatttatct aaatatactg actttaattg tttactagct aaaatctttt tagctggttc
     6361 aagctttagt atagctgata gtatagctcc catagctcta tgactagcta gagcatcatt
     6421 atcaaaaatt aaattttgta actgcccttt ttcgattgcc atgagaactg ctctatgtac
     6481 tgaatttgct ggagttatta ttttttcatc tctctttaat aacataatgc tatttttatg
     6541 gcaatttcta acacaaactc cacaacctag gcatctatcc tcatcaattt ttatatattc
     6601 tttaccatct tccttaacct tgcttatcgc atctattgga catgcattta tacatttacc
     6661 acactttaca caattttcat ggtttatatt tggtataaaa ctggttgtct gtactggatg
     6721 aagatttcca aatcttttag ctgctaccat tgcttcacaa caacatccac aacaattaca
     6781 tataaacgta accccttttc taacattttc tccacattga actaagttat gctcatatgc
     6841 ttggtgaagt agttctttac attctatttt atccacgcgt cttgcaaatt tattatttat
     6901 taaagaattt gctacattgt caaacgtcat acatatatcc ataggagcat cacaagcttt
     6961 tccaacatgt tgcattctat gtctacagta gcacatactt atacctatat gagttgattc
     7021 atcaattata tgagtagccc tttcataatc taaaatacta acctcattct cattagttaa
     7081 aacttcctct tgaacatata ctcttccaag cttcgtttct gttgaataaa ataaatcttt
     7141 gataaaatct tcttctacat tcatatattg atagtaaagt tctgctaaaa gtttttggtc
     7201 tatatcgtgt ctcgttctca tcatagcaaa ctcaaaaaaa ccagccattg gaggaggcat
     7261 tatatatttc ttccctttat tatcttctat atctaatatc agagctttac ttgcaagttt
     7321 atccaatact ctatatgctt cactttcact tacactccaa attttagctg ctgtttttac
     7381 tctgaaaggc tttattggaa gctgagcaac taacttcgcc tcttgttctg tatatagtac
     7441 atttagtatc ttgtataatg tatctgatgg tggagcacct tgtggaaact tgtttattct
     7501 ttcctctaaa cttttataca tattcttagc agttatatga gaaatagtaa tcaccttctt
     7561 tttcttttgt tttccaataa aattaaatat tctttcttct tatatagact cttactttat
     7621 ataataccaa ttctttctaa atagttaaat acctctagca atttattatt agatttctta
     7681 tataaataaa aaaatcgttt aagtagtata ataatagttt atactactta aacgatttta
     7741 ataatagttt atata
//
LOCUS       DAEMAE010000175         7645 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEMAE010000175 DAEMAE010000000
VERSION     DAEMAE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.177"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            30..476
                     /locus_tag="KPH93_002900"
     CDS             30..476
                     /locus_tag="KPH93_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1689677.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNRIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            478..1572
                     /locus_tag="KPH93_002901"
     CDS             478..1572
                     /locus_tag="KPH93_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG1689678.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKIAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCENRSRFASEIISSIRNEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGINSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMISMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTHSQFGCILD"
     gene            1660..2190
                     /locus_tag="KPH93_002902"
     CDS             1660..2190
                     /locus_tag="KPH93_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG1689679.1"
                     /translation="MKSLILYSSLTGNTKKIACSIYEAIQGKKEIKNISELVDDSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASTKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKVKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EANVFAKNMMIKASTM"
     gene            2517..3350
                     /locus_tag="KPH93_002903"
     CDS             2517..3350
                     /locus_tag="KPH93_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1689680.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDNIRNIIFQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICIVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIVRHVTVIIFIDILVFIALLIIGVYKLFKKY
                     N"
     gene            complement(3599..4975)
                     /locus_tag="KPH93_002904"
     CDS             complement(3599..4975)
                     /locus_tag="KPH93_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689681.1"
                     /translation="MKYKIGFLTTSYFMSDKFKKVLKELNDICEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDDKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            5243..6415
                     /locus_tag="KPH93_002905"
     CDS             5243..6415
                     /locus_tag="KPH93_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1689682.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6429..7076
                     /locus_tag="KPH93_002906"
     CDS             6429..7076
                     /locus_tag="KPH93_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689683.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            7078..7587
                     /locus_tag="KPH93_002907"
     CDS             7078..7587
                     /locus_tag="KPH93_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG1689684.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
BASE COUNT         2908 a          816 c         1315 g         2606 t
ORIGIN      
        1 agattataat tatagaaggg aaatttaaga tggattttaa aaatttacag tatgaaagtt
       61 tgtcatatat tatttgtaac ttacaaaaaa actttaaact ttattgtgaa aaatgtttaa
      121 aaccatataa acttacgaat ggtctatatt tctatttaat ttatattaat aaaaatagga
      181 attgttcttt gaatgatgtt tctacagaat ttgaagttga taaagctcat acaacaagaa
      241 ctatatctag gttagaacaa gatggataca tagaaaaaat ccaaaaccct aatgatagca
      301 gagcttttca gttgagagta acagataagg gtgaggaagt attaggggat ataaaaaaca
      361 tattttcaaa gtgggataac cgcataaaaa aagagttctc agatacagaa tataaagaac
      421 tagtaaagaa tttacatgta gtaaaggata taaaaacagc tgtggaggaa gaataggatg
      481 tattgtaatt tattaaaacc aattaaaatt ggaaatttgg aattaaaaaa tagagtatct
      541 tttgcaccta caagcatggg gcttaaattg gaagaaaaaa ttaaaaagtt ttctgatatt
      601 gctaagtcag gagtagcttt gattactcta ggagatgtat ctattagacc tagttttcat
      661 aagatagcta taagcctatc ggatgaagat ggagtcatga aatacaagaa aattgtagat
      721 gaaatacaca attctggagc aaaggtatca gcacaactat tttgttcaga ttatgatgtt
      781 aatttaatta aagatactat gaagatggga attacttcac atgatgagat aaaaaaaata
      841 atgaatgatg gagtaaaaga ttacataaca aatatgccca aagaagaaat taaaaatatt
      901 ataaatttat ttaaagtaac agcattgaat gcaaaaaaag caggttttga catgattcaa
      961 attcatggtg ataggcttgt aggaagtttt tcatcaagta tatttaataa tagaaatgat
     1021 gaatatggtg gaacgtgtga gaatagaagt agatttgcaa gcgaaataat tagtagtatc
     1081 cgaaatgaag ttaaggacat tccaatagac tataaatttg caataagaca agaaaaccct
     1141 cattatggga atgcaggagt tttactttca gaagttgagt attttgttaa aaaatttgaa
     1201 tccttaggta taaacagttt tcatgtaaca cttgcaaatc attcaaaatt agaagatact
     1261 ataccaacca ataatcatcc ttactttaaa gatgaaggct gttttttata tttagctgat
     1321 gaagtaaaga aacacacaaa tcttccagta tgtggggttg gaaaattgtc tagcccagat
     1381 tttatagaaa gtattatttc aaataataga gtagatatga tttctatgtc aagacaatta
     1441 ctagcagata gtaattggtt acagaaagta aaagatggaa gagtagatga gattaaaaaa
     1501 tgctgttatt gtaataagaa atgtgcagat gccttacaga cacattctca atttggatgt
     1561 atcttagatt aaattgtgtt tgtagaaata aaaaatctgt taatattaat atatttcttg
     1621 taagttagat aattatttag ataaatagga ggatacgtca tgaaaagttt aatcttatat
     1681 tcaagcttaa ctggaaatac taaaaaaatt gcctgttcta tatatgaggc aattcaaggg
     1741 aaaaaagaaa ttaaaaatat aagtgaatta gttgatgata gtattgatta tgaaaattat
     1801 gatatagtat ttttaggcta ttgggtagat aagggaattt gtgataaaaa ttcaaaacaa
     1861 gtgcttgaaa atattcacaa taaaaagata gcactatttg gaacaatggg agcaagtaca
     1921 aaaggaagtt atggcgctag tataatagaa aagatagaat caattattcc aaaagataat
     1981 gaaatattag gttcattcat atgtcaaggt aagatagcag aaggattgaa agtaaaatat
     2041 aaagaaatgt taaaactttc tcctgataac gaacatattc gtcaacaatt aaataaccat
     2101 gaggaatctc aaagtcatcc agatgaacaa gaaatatatg aagctaatgt atttgcaaaa
     2161 aatatgatga ttaaagctag tactatgtaa cagttaaaca aagagtttat ataggatata
     2221 gaatcaataa tccaataaaa agtatagcaa atgatttgct atgctttttt tatgaaatta
     2281 aaaaaataat aaaaatactt gactgtaaac cttgtttata cattatttta tagtaaataa
     2341 gatagtttta aatatagaaa aagagatatt attgaaagga gatattattg tatgaaaatt
     2401 tataaaagct acttagtttg attttaagac ataataaaca tacaatttta tagagtgaaa
     2461 gtatttaaga ctatattata gctataaatt aatagaatag agaggtagtg tttaaaatga
     2521 caattaaaga agttgaagaa aaaacaggat tgacacgctc taatattcgt ttttatgaga
     2581 acgagaattt aattaaaccc cttcgtaatg agcgtaatgg ctatagagaa tattcagatg
     2641 aaaatgtaga ggacatcaaa aaaattgcat atttgcgtac attaggaatt actattgata
     2701 atatccgtaa tattatattc cagaaagtat ctttagttga agttgtagag aaacaggaga
     2761 aagttctcga aactaaaata tcagacttag aaaatgcaaa aactatgtgt aataaaatgt
     2821 tagaatctga tgagctaagt tatttgaatt tgaaagtaga gcaatacata actgagttac
     2881 cagaatactg gaataaaaac aaacctgttt ttaaacttga ctctgtaagt tttttatata
     2941 tatggggagg gaaaattacc tggggaatta ttactattgc ttgtttgttg attgcaataa
     3001 cagtatttcc aaagttgcca cctcaaattc ctatccaatg gaataatggt gtagctaatt
     3061 atgtagataa aaaatttatt tatgcctacc ctgtcatttg tattgtattc agaatacttt
     3121 tacgtccatt tatatggaga tggttacaaa tacatgcttt ttatagtgat actattgcag
     3181 attatttaac gaattttatg tgttttgtag ccttgtctat agaaatattc tctatacttt
     3241 ttatttacaa tattgtcaga catgttacag taattatttt tatagatata cttgtattta
     3301 ttgctttact gataataggt gtgtataaat tatttaaaaa atataattaa cttttaaaaa
     3361 taagtaataa atgatagcgt aaaaaataac cactgcaaat tatggattgt agtggctatt
     3421 ttattatttg tatatgaaat tattttgttt ttctaataag ctacgatata ttatagttta
     3481 tttcactgat aaaaggttta ggtattttaa agtaaaaagt tgtatattct aataaaattt
     3541 aaacattttt attctttaaa attagagaaa atacattgct tttattaaaa attaaatatt
     3601 aagaatttaa tttctgcatc ttatttagaa aagataagaa atcaacttta tatatttttt
     3661 ttggccttcc tctttgatta tcgagttttg tatttacaat agttgcaatt cctgctttat
     3721 ataagatact caatattcta ttagcacttc taagagttat gttcatatag tctgctaata
     3781 tttctgctga tacattattt gaatttctat cattaatcat acacatgatt ttagatacat
     3841 ttgtattaga tagaggtata aaagaagcaa ctttattggc tattgaacta tcttcaatat
     3901 tattagaatt tttatttgaa tataaaggac cgattgtatc attagtatca tttactataa
     3961 aagtacagtt tccattcaaa gaagcagatt taccattagc ttgacgagca ttttgctctg
     4021 catgaatatt tgtatttcca atgccccaac ctatatttaa gctatattta atttctttta
     4081 aatcttctga aataaaaaaa tctgtatagt tatttgtcat attgtttaac atttctttag
     4141 tagtgtgtat ctctattttc atatcatttt ttttgacctg aactccataa aatccatgtc
     4201 ttgatagaga atttaatata gtgttatata tttgattttg tatctcatca tttttaaata
     4261 tcaaatcttc atttatgtac tctgaactat ttactgtaac tatacaagtt gcagatttat
     4321 ttttgtctaa gttttgtaat gttatttcat tcaccagaga tttaaatgta ttcaggattg
     4381 attctgtaga tggaaccatg taaatacaat catatccaag cttattaagt tcgcaactaa
     4441 tgatgtaatt tctagtaaaa gaaaggttaa tcttatcatc tcgcaataag tctaaatgta
     4501 tttccaataa tttatcattt aattttttaa agtcatacac aggttgaggg atattaaaat
     4561 ctttaattgt atatggacat ttattagaat ctattaatga atatatatca taataattgt
     4621 tgtcctctct taaaaaatcc ataaatgatt ttgtaaagtc aatatctttt tgactattta
     4681 atagtttaaa taagtattta taaaaatctc tttcatcaag ctttaaaaag tttaatggta
     4741 cttttatgtc ttttatattt attataagtt cactatatgg aactatacca ctacatacaa
     4801 atccatcaaa attatctact tgttcgtaat atatattagg taaattgtta aaaggatttt
     4861 ctcccttaag ctcaataaat gtaatttcgc aaatatcatt taactcttta agaaccttct
     4921 taaacttgtc tgacataaaa taagatgttg tcaaaaaacc aattttatat ttcataaaac
     4981 ctcctttaaa atttcgagag tattgtaact aatcaatcaa tttataacct cattttataa
     5041 tagtagataa aatgcgtcaa tttattttac aaaaggataa ttgaaagaaa aatcagaaaa
     5101 tataaaaaag actgaaaaat atgttgactg gcaggaatga ttgtgctatt ttgtaattaa
     5161 ggactagtct tttaaaattt cctaaaataa tcttaaattt taggaaagaa ttacatttta
     5221 tttaaaaata aaggagatta ttatgaatac atatttaaat gagatttcta agtttgttga
     5281 tgagaataga gaagaaattg tttcattatg gaaagagatt gttaatatgg aaagctatac
     5341 acattgtaaa gaaagcgtaa ataagttggc taagagatta aagtttgagt ttgaaaaaga
     5401 aggtttagat tgcgagttag tagatgttgg agataatggt agtacattga taggtacact
     5461 tggttccaat atagataaaa agccaataat tttttcaggt cacatggata ctgtctttga
     5521 aacaggtaca tttggagaga atccatttaa aataatagaa ggaaaagcat atggcccagg
     5581 tgttttagat atgaagggtg gtataataat ttccttatat gttattaaag cattaaataa
     5641 aataggatac aaagaaagac caataaaaat agtattttct ggagatgaag agataggaca
     5701 taaggattca acaggtgcag atgttatatt aagagaagca aaaggtggtc tttgtgcttt
     5761 taatatggaa actggattag tagacaatag tttgtgtgta ggtagaaaag gaagaatagg
     5821 atgcaatata catgtaaaag gtgtagaaac acatgcaggt aatgattttg aaggtggaag
     5881 aaatgctata gaagaaatgg ctaataagat actacgcata caaaaattga ctaatttaga
     5941 tgttggaact acagtaagtg taagtgtgat taagggtgga agagtggaaa attctattcc
     6001 agaagattgt agtataaaag tagaccttag atttgaaaaa gtagaagaaa tggagaatgt
     6061 taaagaacaa gttagagaga tatgtaaaga aacttacata gaaggaactt ctacagaagt
     6121 taattttata agtgaaatga tgccatttga aactacagaa gatgttatga gatttcatac
     6181 atttgtaaat gaagtgtcaa aagaaaatgg atttggagaa ttaaatgcta agagacttgg
     6241 aggtagttca gatgctagtt atttaactat tgcaaacgtt cctgcaatct gttcatttgg
     6301 tgtaaggggg gaatggaatc atacatcaag agaatatgca gtagtagatt ctatgtttga
     6361 gagggtgaaa ttaatttcta cagttatttt aaacttagat aaatttgagg tataaagggg
     6421 gattttatat ggatgtaaca gctggagata ataaggtaac atggaaaggg tatttatctt
     6481 tatttgtatt gatagtatta ttttcaggag tatttaaaga tagccaaggc ttccttaaag
     6541 cattagattt tgctaatctg acaggttcat ttggtactat tgcagatggt gtagacttta
     6601 tgggtaaagg aggaacggga gcaaaagatg gtttcttatt tgctctgact ttgattccaa
     6661 ctgtctgttt tgctgtaggt cttatagatg tagttgaatc tatgggagga atgaaagcag
     6721 catcaaagat atttaatcca ttacttagac ctctgcttgg catacctgga atagctggaa
     6781 tagcatttgt atcttcattc actagttcag atgtagcctc aataatgacg aaagaattat
     6841 ttgaaagtgg ggaaataaca gatgatgaaa gaactatatt tgtagcatat caatatgccg
     6901 gttcagctgt tatactgaac acaataaata ctcaagctcc acttttacca atagcattat
     6961 tggcattagg tccaataatc cttatagaga tattctgtaa aatacttggt gctaatctag
     7021 ttagattaat cataggtgta agaaataaaa agaataaggc taaggaggct gtgtagtatg
     7081 agtcaacaaa atactggaaa tgcaattgta aatagtaaac ctaagaaaac tgctgttgag
     7141 atatttatgg ggggggcaaa aaaaggtttt tacataggtg ttgaacagat attaccagct
     7201 atgataatgg gttatgtaat agtgcagttt ttacaattaa ctggtttagt tgatatttta
     7261 ggaaatattt ttggaccagt aatgggtatt tttggactac ctggggaatc tgttgtagtt
     7321 ttgatagcag cattctttgc taaggcagca ggagcagcta cagccgcaaa cttgtttgca
     7381 caaggtctaa tcactgcacc acaagcaacc atattaatta tgccatgtat gttaatgggt
     7441 actctagttg gtcattatgc cagaatagtg cttgtagcaa atgccagtac taagtataga
     7501 atgttactgt tggcagttcc tatatttgat gcaatagtgg ggatgttgat agtacgagtt
     7561 ttacttactg ttatgggatt aatgtaagta acataaaatt atagcctaat tataaatgat
     7621 atataagaat taatatatac aagaa
//
LOCUS       DAEMAE010000176         7612 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEMAE010000176 DAEMAE010000000
VERSION     DAEMAE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.178"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(223..372)
                     /locus_tag="KPH93_002908"
     CDS             complement(223..372)
                     /locus_tag="KPH93_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HBG1689685.1"
                     /translation="MPDFYIGTGCVIGTVMTIQDKIVPNLVGVYIGCGVSYSNLGKIN
                     LDLKK"
     gene            complement(712..1524)
                     /locus_tag="KPH93_002909"
     CDS             complement(712..1524)
                     /locus_tag="KPH93_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1689686.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPDKYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIDYAQNRN
                     KETNLNINYLFQSYLNMSYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLRPGGKL
                     ILDVFTINKYNNFEEIKYWEINEEGGFWSNKKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLFEIENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(1893..2795)
                     /locus_tag="KPH93_002910"
     CDS             complement(1893..2795)
                     /locus_tag="KPH93_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1689687.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWNREVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSTGSLNREYKNQLYKMINELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(2845..3306)
                     /locus_tag="KPH93_002911"
     CDS             complement(2845..3306)
                     /locus_tag="KPH93_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG1689688.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSEDYELWSGNIMF"
     gene            complement(3358..3813)
                     /gene="def"
                     /locus_tag="KPH93_002912"
     CDS             complement(3358..3813)
                     /gene="def"
                     /locus_tag="KPH93_002912"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG1689689.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3785..4048)
                     /locus_tag="KPH93_002913"
     CDS             complement(3785..4048)
                     /locus_tag="KPH93_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689690.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(4145..4861)
                     /locus_tag="KPH93_002914"
     CDS             complement(4145..4861)
                     /locus_tag="KPH93_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689691.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISKYLIVTPLGK
                     TGYLVSRFGIPGILAFIITMILLIFFSLTRISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKVQYILFFLPTFWISKAFKEDKYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4858..5541)
                     /locus_tag="KPH93_002915"
     CDS             complement(4858..5541)
                     /locus_tag="KPH93_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689692.1"
                     /translation="MRLKNLILGDMKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLVDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICNS
                     WKKVGGVKL"
     gene            complement(5538..6383)
                     /locus_tag="KPH93_002916"
     CDS             complement(5538..6383)
                     /locus_tag="KPH93_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689693.1"
                     /translation="MITVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(6497..7087)
                     /locus_tag="KPH93_002917"
     CDS             complement(6497..7087)
                     /locus_tag="KPH93_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689694.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            7376..7609
                     /locus_tag="KPH93_002918"
     CDS             7376..7609
                     /locus_tag="KPH93_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG1689695.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         2691 a         1194 c          705 g         3022 t
ORIGIN      
        1 gtactcctta tattgtatca ttctaacatt gatactttaa tattatttta atcttaagca
       61 ttactaattt gatttaaaat tataaacagg tttcaatatt ttaattatgt caactaaatt
      121 tcttctagtt ttaatactct catcaattga tttgcgtgct tgagattctt catcaatagt
      181 attaattaaa tccataaggt attttttatc ttataaaatc atctattttt ttaaatctaa
      241 attgattttg cctaggtttg aatatgaaac tccacatcct atatagactc caacaagatt
      301 tggaacaatt ttatcttgta ttgtcatgac agtacctatt acacatccag tacctatata
      361 gaaatctggc attattctta tcttagactt tgttgtaaat tcttaattac ataattctat
      421 tatctgcaaa tatctttata ttttccttga acctctaaca ttaatataac atccttttta
      481 gctatacatt tttaactaca tacttaattt taataacatt gtttgtttag aaaatatact
      541 ctcgataaat ttcacataga aataataatt caaacattga tttttatata agcaaatttg
      601 taatgtatta aaaataacat aaaagtttcc atttttcaat atgtaagctt ttcttatgtt
      661 attttgttat tagaggtttc aagtttttat ttattcattt tgaaaaaatt attatttttg
      721 taaaactaat cccatagtca tactatcgtc agaatatgct tcacctttaa cattactaaa
      781 aaactcaaca gatttaaatc caatattttc aatttcgaat aagaaacttt cttttgaaaa
      841 atagtgattc caaacataaa acacattttc atctttttct gttattacaa gagtttgttc
      901 taatgtatta taatcattgt atttacaatt atcttgtaga cacatatatt ttttattact
      961 ccaaaatcct ccttcttcat ttatttccca atatttaatt tcctcaaaat tattgtattt
     1021 atttattgta aatacatcta atattaattt tcctccaggt cttaaactat cataaatttt
     1081 ttccattaac aaccttctgt tttcagttga caaagctcca taatcacaat aaattaaagt
     1141 agccaaatca aattcctcat tataactcat atttaaataa ctttgaaaca agtaatttat
     1201 gtttaaatta gtctccttat ttctattttg tgcatagtca atagaacgtt ttgagaaatc
     1261 aattccagtt accttataac ctttttgtgc aaatttctct gcatataaac ctgggccaca
     1321 acctaaatca agtaatttag gatacttatc tggatttgca actgtaacta tccaatttac
     1381 agaatcctcg ataaagttaa aatttctact tgctccttca aaattagtgt ctaagtgtgc
     1441 ttttaataat tgctttgaaa tatactcatc atcccaaaaa ttaatttcac tttgtttata
     1501 aagctctggt ctttctaaat acatacataa tttgtttaac aaaacatacc actccttaaa
     1561 atttattttt aaaataaaaa aatcacagag ttcactctgt gatttaactt caaaaagatg
     1621 agtttaaata agagtaaaat agaaccaaat atttttaagc acaacaataa aactatagct
     1681 tactttgttt gcttattaaa tttgatttta ttcactcttt tccagcaata cttatatcaa
     1741 cgattttatg atatcaaata tcaccgaaca acatcatcct ttcatatttt tattttatga
     1801 attaattata tatttaaatt ttatatttgt caataaattt taattttaat aaattttaat
     1861 catcatagta aaaaaaattt ttttatataa aactaatcaa aaaaagatat ctgtacatct
     1921 cctgagtttt ttaacttttc cttcattatt ttagagtaac tgcttgatag agaatattta
     1981 tatctaagtt cattaatcat tttatacaat tgatttttat actccctatt aagtgaacct
     2041 gtgctatata gtattgataa tttatcaaat aattcaggaa actctttttt aacaaagtca
     2101 aaaaaaacac ctctagttat accccttaag tataaagtcc caggtaaaac ataatgtaca
     2161 ttacattctt ttgcatgtct aaatagtgaa tttatattgt caaaatcatc tgtaatatat
     2221 ggtataattg gcataatatg tagaccaact gatgcgttag tttttctaaa ttcttttaac
     2281 attttaaatc gtctcattga atccacacca tttggctcaa taagcctttg tgtatcttca
     2341 tttactgtgg taattgtaga tgctacattg atatatgtaa tccgagatag cttatcaatt
     2401 aaatcgtaat ctcttaaaat taaatctgac tttgtagaaa taatagctgg tgtcttgtac
     2461 tttatcagca gatttaatat ttctggcata attttatagt cagcttctat tggttgataa
     2521 ctatctgtaa ctcctcctat attgattact tctctattcc atttactgct tttaagttgt
     2581 ctctcaagtt tttctactat atttgttttt acataaatct cttcaaaata attattggag
     2641 ttgatgtact tatgagagta aatagcataa cagtatttac atccatgctc acatccacga
     2701 taaatattta aatcccattt atatggcata ctccttttta atttattaca agctatttca
     2761 caatttattg gaatatattt tattttatta tacaaataat cacctcttta tatataataa
     2821 tattataatg ttaatctata gtaattaaaa catgatattt ccagaccaca attcataatc
     2881 ctcactatat ttacaaagta atatattcca ttcatttatt ggtattcctc cacatgttgc
     2941 tttttcttct aaattatata agcattgctt taaatacatt ttattatgcc aatcatcata
     3001 taaattttct aaatttaaac tatgttcatt agatatatca tttgcaaaat ttctattttt
     3061 ccactttaat atacttgtat atttaagttc attaggttta taatttcttt ttaacatttc
     3121 atcatgtaca atttttgtat aaaatttaaa ttcatctata ctataattca atactttatt
     3181 cactaataga tggtttggag tacctttttt atctatagaa ccttttatag caagtagctc
     3241 tctccattga cttactaatt gatttttagg taatacatca attaaatcct tatgccataa
     3301 tctcataatt taactcctta ctataaaata ttttataaat tcaataacca atctatctta
     3361 caattttaca tgttcaataa ttttatctgt aaaaactata ccatttaaat ggtctatctc
     3421 atgacaaaga cattttgcca taaaacctga acctttaatt tctattttct caccgtattc
     3481 gtttagagct tgcactgtaa ctttttttgg tctttttaat ttaccccata cttctggaaa
     3541 acttaaacat ccttcaacaa caatttgttc cccttcttgt ttaataattt caggattaac
     3601 aagcttaata agtccttctc ctaaatctat aacaactagt cgctttaata ctcctacttg
     3661 gcatgcagct aatccgcctc catttggagt attgtacatg gtctctgcca tgtcatttaa
     3721 tatttctctt atcttattat ctacgttttc tacgtactta ctcttttttc ttaaaatttc
     3781 atcatcaaat gttcttatct ttcttatagc cataatattc tcccctactt atctgtgctc
     3841 ttgattttct ttcactttct tcaaataaaa atacactttc aattggttca ttaaaaattc
     3901 ttgaaatatc atatgccaac catattgaag gttcattttt accagtttct atagcattta
     3961 tagcctgtct agatgttcca actaatcttc ctagttgctc ttgtgtcaat cctaattttt
     4021 ctcttaatat ctttatttta tttttcatat ttgctccatt catgtaaggt taactttaca
     4081 ttttaaatat aacatttttc aacattgctg tcaagtcaac cttacattta tatagtaaat
     4141 tttcttattt aactattttt ttactaaact ttttaaataa taataaaatc catattaatg
     4201 atataacaat tgaaataaac atatagatat atttatcttc cttaaatgcc ttcgatatcc
     4261 aaaaagttgg taagaagaac agaatatatt gcactttatt taaaataaaa aatggtgcta
     4321 aaatacccat cataaaaaca cctgagaatt tcgtaattac cattccttct agtttatttg
     4381 atgataaaga aatcactaat attgatataa taactccttg taataaaact aataaagaaa
     4441 ttgataaatt taacaagaaa gaaattctag ttagagaaaa aaagataagt agaatcatag
     4501 taataataaa tgctaagata cctggtattc caaatcgtga aacaagataa ccagttttgc
     4561 caagcggagt tactattaaa tattttgaaa tactgtcatc aatttctccc aatatgacca
     4621 ttgtagaagc aaaaaagaac ataaaaggtg taataaaagc taaaagtaaa tcaaacatca
     4681 agaaatacgg ctctaaattt aattgataat aaaacttgtt aagtgatata ttctgtatta
     4741 atggtattcc aaattttata aatactccac ataatattgg agaaacacat actataaaaa
     4801 gcatagcatc tctcttaatt tgttcaaact cttgtttaaa cgcatttata atctttatca
     4861 tagttttact ccacctacct ttttccaact gttacaaata aaataataag ttataaaata
     4921 aattattatt ataattgata ttaaaattaa aatattaatc attataaaat ttttatctcc
     4981 actaataaga gaaatacata tattaaaagg atacaaattt gcatatgatt ttgtatcaac
     5041 aagtacattg agtattggtg gaataaagca tataatctca ataggaatac ttaaaacaat
     5101 aaactgattc aaactagatg ctttacttgc aagtatcaat ccaagcaaag aaaatataat
     5161 agaagaaaaa aaagtaccta tagttgatat tataatatta tctaaattta atgtaatagc
     5221 aataaacata gctacaatac tagatattac tcctaatgaa attacctttg ataaaatata
     5281 ctcagatact tttactggag atacagctaa ggaatttaaa accctttgac ttttctcaag
     5341 aagtacaata gctcccataa aaaacaatcc cattgctgct gggtcagaat aaatcataat
     5401 tatagctatt ttttctctca taaaagttgg aaatacattg ataatacaaa tatatataat
     5461 acttaaaaaa agatataaaa aataaaagcc atacttaaat tgaaacttca tatctcctaa
     5521 aataagattt tttagcctca ttgtaaattc ctccctgtta tctcaataaa aatatcattt
     5581 aatgttggtt cactactatg tattgataaa agtttatttt tctcaataag catattcaaa
     5641 tttttatcat tagcagtatt attcagaaaa cattcagaag ttttttctcc attatcaaag
     5701 taagtatatc taactttaat agctcctttt gacataataa gattatgtgg tgtatctaaa
     5761 gcagatattt taccatttac aatgaacgct actctgtcac aaagctctgt tgcatctaac
     5821 atgttatgag ttgttaaaat aatagtttta cctttagatt tttctgataa aattatatct
     5881 ttcataacct tgctatttga tgggtcaaga ccacttgttg gttcatctaa aaataaaata
     5941 tctggattat gtaataaagc ttttataaaa tttaatctag atttcatacc ttttgaatac
     6001 tcagataccc ttttatttgc ttcattttcc agtcctactg attttaataa ctcatcaata
     6061 gatagtaatt ttttagaata tagagaccca aaatatttta aattttctat tgcagtcaat
     6121 ttttcatata aactaggaaa ttcaaaatca acgcctatat tttcataaaa tttatttgag
     6181 tgtcttttac tctctactcc atttacaatg acacttcctc cataattagt tatcattcca
     6241 attagaatct tttgtaatgt agattttcct gcacctgatg gtcctaaaaa tcctagtatt
     6301 tccccttttc ctacttcaaa gttaatattt tgtaaaaaat ctctatccgt ataactaaat
     6361 gataaatcat caactgtaat cattcaatat caccctttca actttttgac tattttgact
     6421 atatggtcaa taaaatataa tttttttgtt tgttaatact ttttaataaa agttattagc
     6481 aaacaaaatt taaattctat tccataagtt gtataactaa tccgtttatc attaatctta
     6541 aagcatcttc aaaacaatta cttccaacct cgcgcttata taacatggta agaaaaactg
     6601 ctctaaatgc tccactaaag ctttctatgt ctttattttt tgcatttggt atgcaagata
     6661 taatctctcc aatttcaaag tcatcatgtc taaagtgttc ttcaattata gaatctggta
     6721 actttctcac caatatctca aattctccat ttatcatgag atttaaaatt gaagtactat
     6781 caaccatcct atagaatttc ataaatatat ctgtcaattg ttcacatgta atactttcat
     6841 ctactctatt tatctcatat aagatttctt tttgtataga gtcatgtata tctctcaata
     6901 cctcaaaaaa gagcaattct tttgattgaa aaaatagata aaaagttcct tttggtattt
     6961 ttacccgttt tacaagctca tcaacagtag tttttctaat accatattgc tccatacaca
     7021 atcttgcttc ttctttaagt ctttttatta tatactctct ttctttatca gaatacgatt
     7081 taggcatctt tttcctccaa tttgactaaa ataatttata tagtcaatat aatacttttt
     7141 ctatctaatg tcaatagtat tattagtata tttaaaaata agcttaaaaa ctttcgaaaa
     7201 ttctgaattt tatatagcat tttttattat ctagtgttat aattaagata taaataatat
     7261 attattctaa ttttcatatc tatttgctta tttcttaact tagtttctaa tagaagtaat
     7321 catatttatc tttttaaaag tttcaacttt aataaaaaat aaaggagttg attttatggc
     7381 tactactaca aaaaaccgtt tttacgatta tgttcaagaa aacttaaata aatatgttga
     7441 agtacattct tactttagca gttgtcctct atatggtgaa attatagaag cagatagctt
     7501 atcaattaca ctttgctcaa gatatataga tgaatctaat ccaactttaa atgaaactta
     7561 cacattatat ttaccattaa gttcaataat atctattaga acactataaa aa
//
LOCUS       DAEMAE010000177         7583 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEMAE010000177 DAEMAE010000000
VERSION     DAEMAE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.179"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..343
                     /locus_tag="KPH93_002919"
     CDS             32..343
                     /locus_tag="KPH93_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG1689696.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFK
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGNEIEQL
                     T"
     regulatory      436..528
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            628..1422
                     /gene="thiD"
                     /locus_tag="KPH93_002920"
     CDS             628..1422
                     /gene="thiD"
                     /locus_tag="KPH93_002920"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG1689697.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAVT
                     AQNTKGVFDVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKHLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGKEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1446..2234
                     /gene="thiM"
                     /locus_tag="KPH93_002921"
     CDS             1446..2234
                     /gene="thiM"
                     /locus_tag="KPH93_002921"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG1689698.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKSTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2257..2889
                     /gene="thiE"
                     /locus_tag="KPH93_002922"
     CDS             2257..2889
                     /gene="thiE"
                     /locus_tag="KPH93_002922"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG1689699.1"
                     /translation="MIDKEFLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIEVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGIGSIFTTSTKLDAKSASFETVKEIKEK
                     VDIPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3028..3330
                     /locus_tag="KPH93_002923"
     CDS             3028..3330
                     /locus_tag="KPH93_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1689700.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGNIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVAIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3396..4145)
                     /locus_tag="KPH93_002924"
     CDS             complement(3396..4145)
                     /locus_tag="KPH93_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689701.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVAILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIENLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCASFSLAMWRLKKEMVS"
     gene            complement(4139..5101)
                     /locus_tag="KPH93_002925"
     CDS             complement(4139..5101)
                     /locus_tag="KPH93_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689702.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNSGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMNVRENSIFISVNDSRTAF
                     TLINKWLLEHDIKFDAIEIVEPSLEDVFLALTSSKKDLKEGWEC"
     gene            complement(5105..5563)
                     /locus_tag="KPH93_002926"
     CDS             complement(5105..5563)
                     /locus_tag="KPH93_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1689703.1"
                     /translation="MSYKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHIGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5919..6275
                     /locus_tag="KPH93_002927"
     CDS             5919..6275
                     /locus_tag="KPH93_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1689704.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVIGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6346..7062
                     /locus_tag="KPH93_002928"
     CDS             6346..7062
                     /locus_tag="KPH93_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689705.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIKALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7055..>7583
                     /locus_tag="KPH93_002929"
     CDS             7055..>7583
                     /locus_tag="KPH93_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689706.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEVNKFKNS
                     KFIMNLAIGDFGGIFYGIGILSNLGFIQSILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQIATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFIS"
BASE COUNT         2961 a          875 c         1249 g         2498 t
ORIGIN      
        1 ttatatattt aatgttagga tgtgatttaa gatgtatttt acatatatta taagatgtaa
       61 agatgattct ctatacactg gatataccag taatatagtt agaagaatga atgaacataa
      121 gttaggaatt aattcaaaat acacaagagc aaaggggttt aaaaaactag aagtttattt
      181 tgtaaccaac acaaaaagca atgctatgaa gttagaatac tatataaaaa aattaactag
      241 aaataaaaaa ttgtctataa taaaaaatcc aagtatactt ataaatttaa ttgataataa
      301 agaggattat attattggta atgaaataga acaattaaca taaatattta tagtaacatt
      361 ataatgagtt ggtataatgt aaaaacttga caataaatac tattgtaata taatgaagtt
      421 ataaatatag taaattaaaa tagctagggg agccaagagc tgagaggtat accgaccctt
      481 acacctgatc tggataatac cagcgtagga aagcttagta aaaattcaag tatgtattat
      541 ttgtgatata tgctatttct aagctatacc tattgtaggt atagcttttt taattaagtt
      601 atttaaataa aactttagga ggcaacaatg agtaattata aaataccaac acttacaata
      661 gctggctctg attcatctgg aggagctggg attcaggctg atttgaaaac gtttagtgca
      721 attggtactt atggtatgag tgtgataaca gctgtaacag cacaaaatac taaaggagtt
      781 tttgatgtag aggatttaaa taagaaaata ataaaaaaac aaatagaggc agtctttgaa
      841 gatatacctc caagagctgt aaaaatagga atggtttcaa gccctgagat tatacttgaa
      901 atagttgaga atctaaagaa atacaatcca aaatatttgg ttgtggaccc agtaatgata
      961 tctaaaagtg gatattatct attaaagcca gaggcaaaag aaaacttaat taagcatttg
     1021 ataccacttg catacataat tacaccaaat ataccagagg cagaagaaat cacaggaata
     1081 aaaatacata atgtagatga tatgaagaga gtaggaaaag aaatactaca acttggacct
     1141 aaatttgtac ttatgaaagg aggtcattta gatggagaag cagtggatat tttagttggt
     1201 aaaaatatat ttaaagttta taaaagtgaa agaattgata aaaaaaatac acatggaaca
     1261 gggtgcacat tatcttctgc aataacatct tatctagcat taggatatga aataacagaa
     1321 gcagtaaatc taagtaaaat ttatataaca gaagcaataa aaagaagctt tgatataggt
     1381 catggagtag gaccagtaca tcatttttat aaatttgagt aataaaaaaa tattggaggt
     1441 agaatatgta taatttaatt aaagatgtaa aaaaattaaa tccattggta attcattata
     1501 ctaataatgt aactataaat gattgtgcta atgtaacttt agctgtagga gcaagtcctc
     1561 ttatgagttt ttcatatgag gaagtagaag aaatggtaag tgtagcaaac tctgtagtaa
     1621 taaatatagg aactatgaac tcaaatatgt tggatttatt cctattagct ggaaaagcag
     1681 caaataagta caataaacct gttgttttgg acccagttgg agtatttgca agcaaagcga
     1741 gagcagaact tacaagtaga cttttaaatg aagtcaaatt tagtgttgtt aaagggaatg
     1801 tgtcagaaat aaagtttata ggtggcttta atgtaagggg taaaggtgta gattcatttg
     1861 atgaagaaga agattctact gagataataa gaaagattgc tgaaaagctt gaatgtgtag
     1921 ttgtagcaac tggaaaaata gatataataa ctaatggtaa aagtacatat aaaataaaca
     1981 atggaactga taaattaaaa ggaattacag gaactggatg tatgactgct agcttgattg
     2041 caagttttat ggctgttact gagaatatat tagaagcagc aacaatgggt gttcttacaa
     2101 tgtctttaag tggagaattg gctaatttaa ataatccgcc aataggtaca tttaaagaaa
     2161 atcttatgaa tgctatttat caaatggata tagatacttt aagtaaaaat tctaatattg
     2221 aatttttaaa ttaataaaga gtaagggaga cttaaaatga ttgataaaga atttttaaaa
     2281 aaatgtttaa agttgtatct agtaactgac tctgaaatgc ttaaaggtag agacttttat
     2341 aaatgtcttg aagatgcaat atcttctgga ataacaactg tacaacttag agaaaaaaat
     2401 gcttctggaa gagagttttt aagaaaagct atgaaactta gagaaataac taaaagatat
     2461 ggtgttaagt ttataataaa tgatagagtg gatatagcac ttatttgtga tgcagatgga
     2521 gtacacgtag gacagagtga cattgaggtg agagaggtta gaaagcttat aggaaacaat
     2581 aaaattctag gagtatcagc aagaacttta gaagaagcta tttgtgcaaa aaatgatggc
     2641 gcagactatt taggtatagg gtctatattc acaacatcta ctaaattaga tgcaaaatca
     2701 gcttcgtttg aaactgttaa agaaattaaa gaaaaagtag acataccatt tgtactaatt
     2761 ggaggtataa atttagacaa tatagataaa cttaaatgtt tagaaagtga cgggtatgct
     2821 ataatatcag ctatattaaa agctgaagat atctctaaag aagtggaaaa gtggacatta
     2881 aaaatataaa attggctctg gtagtgaaaa aaatggaaaa atttgatata atctaagagt
     2941 aaaatttaag taattataaa tgataaactt ttctacatat actttttaat caaaatatat
     3001 gtagatttgg ttaaaaagga gctataaatg attaggatat tgttagtgtg tgttggaggc
     3061 atgtcttcta ccttattggt aaataaaatg gagaaggatg caaaaaaaag aaatatagac
     3121 tgtaaaatat gggcagttgg agaaggaaat ataaaatcgg agcttgataa ctttgatatt
     3181 ttacttcttg gaccacaact tagatttatg cttgatgatg taaagtctat agttggagat
     3241 agagctccag tagctataat agatatggta aactatggta cttgtaatgg tcatgctgtg
     3301 ttaaattcgg ttttagaaat attaaaataa atgataaaag tggattctat ataatagagt
     3361 ccacttttat tatttgttat taataggttt aaaacttagc ttaccatttc tttttttaat
     3421 ctccacatag caagtgaaaa acttgcacag cacaatatta aaaatagtag gataacaggc
     3481 agtatatctc caataagaat agtctcacca aatattaagc tacgtaaagc gttaataaca
     3541 tgggtaaatg gattgagcat cactgctact tttaatcctc cagataaatt ttctataggg
     3601 aaaagagctg aacttaaaaa gaaaatagga agtacaattg cagtcataat tgtttcataa
     3661 actatctcat ttggcaataa taaacttata gaataagcta gacttgataa aaagaaggct
     3721 gtcataaata tcagcactat cataagtagg attccaccaa taccagattc tatccttact
     3781 gaaaagaata tacttactat acacaaaata gctacttcta tgaaggaaac taatatagct
     3841 tctaataatt gaccaaggac gattgaatat ctactgatag gtgcaattaa gactctgtag
     3901 aaacttccac tgttcttcat tataaagttt ataataccac cactactaca agaagaaaac
     3961 acaacaagta caataatacc tggtagtata aaagcagtat aattattgat atttatatta
     4021 ttcatagttt ggtttgcaat agaactataa agcacaagcc aaagaaaagg ttgtaatata
     4081 gtaactacaa atgagattgg atttttaaaa cgccacttca tactacgcca taagatattt
     4141 aacattccca tccctccttt aagtcttttt tagaacttgt aagtgctaaa aatacatctt
     4201 cgagacttgg ctctacaatt tctattgcat caaattttat atcatgttct aaaagccatt
     4261 tatttattaa agtaaaggca gttctactat cgtttacact tataaaaatt gaattttctc
     4321 gtacgttcat aaactttact aaaccagtac ttttaattgc atctttatat ttttttatat
     4381 cttcagtatt gtggaaagtt atacgtagta tattttgtct aatataacta cgtaaacttg
     4441 atggtgttcc ttgtgctaaa tcttttccat tcttcataat acaaatgtta tcacttaatt
     4501 gttcagcttc ttcaagataa tgagtagtta gaaatatagt tgtgccatac tcatctctaa
     4561 tttttaataa catatcccac atagattttc ttgaatctac gtccatacca accgttggtt
     4621 catctagaaa tagaatttta ggagatgata ccatattcat agctatgtcc aatctacgtt
     4681 ttaccccacc tgaatatgat gaagtaggat attttaagta actagataag tcaaacttat
     4741 caattaaaga gtctattctt tgttttgcaa tctgtggttc tactttatac attttacttt
     4801 gaaataccat attttccata agagataagt gttcatcaat agagatttgt tgtgctacac
     4861 aagcaatctg agtacgtatc caagaaggat tatctactaa atcttttcca aacattgtta
     4921 catttccaga tgtaggcttg taaaaagtag tcaaaatatt gataagagaa gatttaccag
     4981 caccattagg accaagtaaa gaaaaaattt ctccagaatt tacattaaag cttagaccat
     5041 ttaaagcttt cacaccattt ttatattctt ttactaaatt gtctatttca atcgctaaca
     5101 tatattattc ctcctcttta agtggaaata ttatctctgt aatatagttg tctgggtttc
     5161 cttttatcaa catacctggt cctttaataa atacttctct ccaaggagtt tgtattttta
     5221 tattattttc atgaatatat ttaaatatca tttcatacgt ttttggcaaa ttactataag
     5281 aacctatgtg agtaaaaaat aaagcttttg ttttaggaaa ttcttttgta gttataccca
     5341 tacgatttgg agtctcagca gttggaacac ataattccat ttctgcaaca ccagtttttt
     5401 ggtcaacatc atagtagcaa aagaatggtg ccccgttaga ctttcctttt atagctttaa
     5461 atacatttgg aaaatatttt gttgcctcag tcataggtcc attataacgc atggtagcca
     5521 ctgtgactga ttcgatattt ttgatttcaa ttttataact catttgttta cctcctaatg
     5581 tgtgttcata ttcttactag atttttaagc attaatacat ataagatttt gattagttga
     5641 aaacatataa tcctatctcg tttgcttgtt ttcaagtcta acataacata aaatatatat
     5701 cctctcattt gatgctccat tttgtaaagt tattatttaa gctttacttt agatttttta
     5761 gtaaattaaa ataaagcaat ttataaaata agtatttaca aattaagtgt attaatcgta
     5821 taatacactt aataggatac tagataaggt ctgaaataaa ggcgtatttt tttaaagtaa
     5881 gtgtactatc gtattaatac actagaatag gaggaatcat gaattttgaa ttagataata
     5941 caactcccat ttatttgcaa atagtaaagt atataaaaag acaaatagta ataggagagt
     6001 tgaaaccagg agagacaata ccatcaagaa gagaaatggc acttaatcta aaagtaaatt
     6061 taaatacagt tcaaagagca tataaggaga tgggtgatat gaatataata aatactttta
     6121 aaaattatca aagcagtgta acagttgatg aaaatatatt aaaaaattta aaattagagc
     6181 ttataaatga atcactatct gtttttatag aagatatgaa agcaataaat gttagcaaag
     6241 aagaagtttt gaaaataata gaagataagt attagttttt aacaaataga aaacttagtt
     6301 tagaaagttt atttataagc atattggatg tggaggatag taaatatgat agaaataaag
     6361 aatgtcagca aaacatataa aaggatgcaa gggcttaaaa taagaaagat aaaagcttta
     6421 aaaaatgtaa gttttaatat agaaaaggga aaaataactg cattacttgg tataaatggt
     6481 gttggtaaat caactatgtt aaaagcaata gcaggactta taaacattga tagtggagag
     6541 attcgtatag atggagaaaa aataaatgaa aaggtataca ataaattggc ttttgttcca
     6601 gatgtacaat ctcattttag caatacaaca ataaaagaaa catttgaatt tatggaaatt
     6661 ttttattcaa agtggaataa agaaaaatca aaagaaatga tggacatatt taaattggat
     6721 gaagatgaaa taattgataa cttgtcaaaa ggaaacattg caagagtaaa attaatactt
     6781 ggattttgcc aagacccaga gtacatactt cttgatgaac catttacagg aattgatttg
     6841 tttaagaggg aagagtttat aggagtaata gcgcaatata tggaggaaaa tcaagctatt
     6901 atcataacta ctcatgagat agtagagata gaaagtttag ttgatgaggt cgtaattctt
     6961 gatgaagggc agattataac ttcatttaat gcagaagaat taagagaaag agaaggtaaa
     7021 tctatattag ataagatgag ggaggtatat aagaatgaat aaacttctat atctaataaa
     7081 tgtagatttg agaagaagca ataagtttta tatagcttat atatcatttt tttcattaat
     7141 tacacttagt ttaaatttat tcgaagttaa taaatttaaa aattctaaat ttattatgaa
     7201 tttagcaata ggagattttg gaggtatatt ttatggaata gggatactta gtaatcttgg
     7261 ctttatccaa agtatattgt tctttgggat gttgggactt tttatatact atatttttat
     7321 gtggaaaaga gagttctttt caaataatca aagtatttat acacttatga tgttacctca
     7381 aaataaattt aagatttgta tatctaaatc aatagctttg ataacaatga tatatggttt
     7441 tttgataagt cagatagcaa cattatttat atgtaaatat atatttaatt tcatttttag
     7501 aaatatgcca ataatcaata tgaactttgc caaagattta tattatttaa atttgaagag
     7561 tataccaatt gattttatct ctt
//
LOCUS       DAEMAE010000178         7548 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEMAE010000178 DAEMAE010000000
VERSION     DAEMAE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.180"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..1347
                     /gene="nifV"
                     /locus_tag="KPH93_002930"
     CDS             154..1347
                     /gene="nifV"
                     /locus_tag="KPH93_002930"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG1689707.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1372..3297
                     /locus_tag="KPH93_002931"
     CDS             1372..3297
                     /locus_tag="KPH93_002931"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG1689708.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIDGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDDKDYENISLLDNLEIPNILDNLGSGILEVKNTTKRTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3319..4314
                     /locus_tag="KPH93_002932"
     CDS             3319..4314
                     /locus_tag="KPH93_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG1689709.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIIDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4643..5116
                     /locus_tag="KPH93_002933"
     CDS             4643..5116
                     /locus_tag="KPH93_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1689710.1"
                     /translation="MVGSMEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRVCENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKKVVTKLLEKMSKNVENEWFKVKR"
     gene            5150..5644
                     /locus_tag="KPH93_002934"
     CDS             5150..5644
                     /locus_tag="KPH93_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689711.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKIYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPKEYLGLNWSSNKKAMVIHRMAVNPSFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLIKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5741..6262
                     /locus_tag="KPH93_002935"
     CDS             5741..6262
                     /locus_tag="KPH93_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1689712.1"
                     /translation="MIEFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYEKVHFNNF"
     gene            6451..6762
                     /locus_tag="KPH93_002936"
     CDS             6451..6762
                     /locus_tag="KPH93_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1689713.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6790..7476
                     /locus_tag="KPH93_002937"
     CDS             6790..7476
                     /locus_tag="KPH93_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1689714.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIATYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDIIRNYVILYNNSSYTFEVVISKIMIGTVFGIVLGLVKYRSRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3018 a          754 c         1464 g         2312 t
ORIGIN      
        1 taatagatta caagatatga aaagctaaaa aaataaattg caaatgaaat taaaatgaat
       61 aaaaaataaa aaaataatca taagttagag gtatcaatga aaaataagac ttaatatttt
      121 taaaattaaa agtatactaa ggggggcatt aatatgtgcg ttatatcaaa agatagagca
      181 aaggaaataa aaatagtaga tactacttta agagatggag agcaaactgc tggggttgta
      241 tttgcaaata gagaaaaaat aatgattgct gaaatgttaa gtgacctagg tgttgaccaa
      301 attgaagttg gtatacctac tatgggtgga gatgaaaaaa atgtaataaa gcacatatgt
      361 agtagaaatt taaaatcaga tataatggct tggaatagag cagtaataaa agatgttgaa
      421 gaatctattt cttgtggtgt tgatgcagta gctatatcaa tatcagtatc tgacattcat
      481 atagaaaata aattgagaac ttctagaggc tgggttttag aaaatatggc taaaacagtt
      541 gaatttgcaa agaaaaatgg attatatgtt tcagtaaatg gagaagatgc ttcaagagca
      601 gatatagact tcttaactga atttataaat gttggaaaac aagcaggagc agatagattt
      661 agatactgtg atacagtagg agttatgaat cctttttcta taaagaatgc aatagaaact
      721 ctttatgaaa gaactaattt tgatatagaa atgcatacac ataatgactt tgggatggca
      781 actgcaaatg cacttgcagg aatagcagca ggagcaaatt atgttggtgt tacagtaaat
      841 ggattaggtg aaagagcagg aaatgcagca ttagaagaag ttttaatggc acttaagtgt
      901 gtttataaat gtgatttaaa taatatagat actagaaaat ttagaggaat atgtgagtat
      961 gtagcacagg catcaggaag aatccttcct acatggaaac cagtagttgg ggataatatg
     1021 tttatacatg aatctggtat acatgcagat ggagctttaa aagacccaca taactatgaa
     1081 ccatttgacc caagtgaagt aaatcttgaa agaaaaatag taataggtaa acattcagga
     1141 agagctgcag ttataaataa gttgagtgaa tatgaaatgt atatttctcc agaaaacgct
     1201 accaaactat taaatgctat aagagctaca tctataagat taaaaagaag tttaatggat
     1261 aaagaaattc ttcaattata ttgtgatata ctagcacatg agaaaggcac aacagaagaa
     1321 gaagcggtta gaggttcata tatttaagat ataattgttt aggaggaaaa tatgggagat
     1381 aatatagttt ataaaataat taaaaaacat atagtagatg gagaagctgt tgctggaagt
     1441 tcaattggta taaaaataga ccagacttta acacaagatt ctactggaac aatgacatat
     1501 cttcaactag aagctatggg aatagataaa gtaaagacta aaagaagtgt ggcatttgta
     1561 gaccataata tgttacaaca aggatttgaa aatgcagacg accataagta tatacaaaca
     1621 gtggcagata aatatggagt ttatttctct aagccaggaa atggtatttg tcatcaagta
     1681 tttttagaaa gattttcaac gccaggagat acacttttag gttctgatag ccacacacct
     1741 actgctggag gtgtgggcat gatggctatt ggagctggtg ggttagatgt tgctttagcc
     1801 atggcaggag gagcatacta tataaaagca ccaaaagtct gtaaggtgaa tctagttgga
     1861 aaattaaata atatggtatc atcaaaagat ataatattag aagtgttaag aaaacaaact
     1921 gtaaaaggtg gagtaggtaa agtttatgaa tatggtggag aaggagtcaa aagcctatct
     1981 gttccacaaa gagctacaat aacaaatatg ggggctgaac ttggagcaac tacttcaatc
     2041 ttcccaagtg atgaaaaaac actagaattt tttaagagtc aaggaagaga agacgcatgg
     2101 atagagttaa aaccagatgc agatgcagtt tatgatgaag agattactat aaacttggat
     2161 gaattaaaac cattagctgc aaaacctcat agcccagaca atgtagatga agttgaaaat
     2221 ataggcaaga ttaaaataga ccaagtggca ataggaagtt gtacaaattc ttcttatgaa
     2281 gatttaatga aagtagcaca aatcctaaaa ggaaataaag tacataagga tgtgagcttg
     2341 gtaatagcac caggttcaag acaagttatg gagatgattg ctagaaatgg ggctttggca
     2401 gatataataa gtgctggagc tagaatacta gaaaattctt gtggtccatg tattggtatg
     2461 ggtcaatccc caggtactga ctctgtgtct ttaagaactt tcaacagaaa tttttatggt
     2521 agaagtggga ctttgtcagc acaagtatat ttagtaagtc cagaagtggc agcagtaagt
     2581 gcaatcaagg gagtacttac agacccaaga gaatttgata ttaaatttac aaatttagat
     2641 gtaaatgaat ttttgataga tgacagcatg ataattaaac ctgctgatgt aggaagtgat
     2701 gtagaagtag ttagaggacc taatataaaa cctttccctc ttaatacaga gctaagtcag
     2761 tctatagatg gaaaagttat attaaagaca gaagataata taactactga ccatataatg
     2821 ccatcaaatg ctaagttact tccttttaga agtaatatac cgtatcttgc aaactattgt
     2881 ttcaacactg tagatacaga atttcctcag agagcaaaag acaataatgg tggatttata
     2941 gttggtggag ataattacgg tcaaggttca agtagagaac atgcagcttt agcaccatta
     3001 tatctaggtg ttaaaggggt aatagttaag agctttgcaa gaatccacaa ggctaacttg
     3061 ataaatagtg gtataattcc aatggagttt tgtgatgata aagattatga aaatatatct
     3121 cttttagata atctggagat accaaatatt ttagacaatc taggtagtgg aattttagaa
     3181 gttaagaata caactaaaag aacttctttt aaggttaaag tagaattatc agcaaaagaa
     3241 gttgatgtat taaaagctgg tggaaaatta aactacacta aaaatcaagc taattaatat
     3301 gaaaagaggg aatattgaat gtataaagtg acacttatac caggagatgg aataggacca
     3361 gaagttgcga aagctatgaa aaaggtagta gaagctactg gagtagaaat agaatgggaa
     3421 gaagttaatg caggagaggc agttattgaa gagtatggta ctccacttcc tgaatatatt
     3481 atagatagta taaaaaagaa caaaatagct ataaaaggcc caattactac tcctgtaggt
     3541 aaaggattta gaagtgtaaa tgtaacttta agacaggcat tagatttata tgtcaattta
     3601 agacctatta aaagttttaa ggggataaaa tcaagatatg aagatgttga tttagtggtt
     3661 gtaagagaaa atacagaaga tttatatgct ggaatagaac ataaaatagg tgattatgca
     3721 gctgaaagta taaagataat aacaagaagt gcaagtgaaa gaatcattga ttttgcatgt
     3781 aactatgtta aagataataa gagaaaaaaa gttacagcta tacataaggc aaatataatg
     3841 aaaatgtctg atggattgtt tttagatgtg tttagagaag ttgcatcaaa acatggtgta
     3901 gaatatgatg atttgattgt agatgcagca gctatgaact tagttttaaa tccagaaaat
     3961 tatgatgtaa tggtaatgcc aaatctttat ggagatatac tatcagattt aggtgctggg
     4021 cttgttggag gtttaggtat aattccaagt gcaaatattg gtaaagattg tgcgatattt
     4081 gaagctgttc atggaagcgc tccacaaata gcaggtcaaa ataaggctaa tccaacagca
     4141 cttattcaat catcagtaat gatgcttaga tacttaggcg aatatgaaaa tgcacaaaaa
     4201 atagaaacag ctttagaaaa agtatttctt gaaggaagta aattgactgt agatttaggt
     4261 ggctcagctt ctactacaga gtttgcggat gaggtgtgta agtatattgt atagatttta
     4321 tttagggaaa aagtaaaata tagattaaag gaaagaggct ttctcaaaat agagataagc
     4381 ctttttcttt attgaaatga taaaatatat ttgattttat acaaaagaga gttcctcata
     4441 aacgaaaaag ttaattttgg gatatccatt ttaattttaa aattaattga ttaatgtgca
     4501 aagaaagatt aaacaaattt attattttta attgaacttt caaccaaaat atggtaaaat
     4561 ttaaaaaagt tgaactttca actaaaaata aatatattga ttatgtgaaa ttctataaaa
     4621 caggaactct ttgactttag tcatggtagg ttcaatggag gtgtgtttga ttaagacttt
     4681 agatagtaat atattaagag aagtaggtac tttatctaga gcagtaaatt caataaatga
     4741 tattaagtac aaggaattaa agttacaaaa aggacaattt acatttttga caagagtctg
     4801 tgaaaatcct ggtataaatc ttgtggaatt atcaaatatg ttaaaggtgg acaaggctac
     4861 aacaaccaag gctatacaaa aattaataaa ggcaggatat gtagataaaa agcaggataa
     4921 gtttgataaa agaggatata atttaactcc aactgataaa tctttggaag tttatgagtt
     4981 gataattgaa gaagaaaata gaagtataga gatatgtttt gataatttta cagatgaaga
     5041 aaaaaaagtg gtaactaagt tattagaaaa aatgagcaaa aacgtagaaa atgaatggtt
     5101 taaagtaaag agatagtaaa taaatacatt aacttaagga gctattttta tgattagaaa
     5161 agcgaatatg aatgatttag aaagcattat gaaaattata aaatctacag tggaagaaat
     5221 gaagatttat aataatacac aatgggatga aaactatcca ttagaaaaag actttgtttc
     5281 agatattaag aaacaagatt tatatattta tgaagtggat ggagaggtag cagggtttat
     5341 atgtcttaat tatgaagagc caaaagaata tttaggttta aattggtcat ctaataaaaa
     5401 agccatggta atacatagaa tggctgtaaa tccaagcttt agaaagacag gaatagcatc
     5461 taagttagtt gattttgctg aaaaattagc tgtagaaaat aatgtatcat acttaaagag
     5521 tgatacatat tcaataaact caaaaatgaa tttattactt ataaagtgtg gttttataaa
     5581 aatcggagaa atgagttttt taggaaagga gaaatctttc tactgttatg ataaaatatt
     5641 ataaaaagca tttataatac aaggggtaaa taatagattt tcagcattag taagggcata
     5701 atataaccta gtgtatgcta ttatgaaagg gatgatttat atgattgaat ttctaaagaa
     5761 aagaagaagt attagaaaat acaaggatgt tgaagtagaa aaagaaaaat tagataaaat
     5821 attaaaagct gctcttttag ctccatcatc aaaggggctt agaacatggg aatttatagt
     5881 ggtagatgat aaagaaaagc taattaattt atctcaatgt agaactaaag gtggtggatt
     5941 tttcttaaaa aatgcacctt tagctattgt aattattgca gataaagaaa aaaatgatgt
     6001 ttggatagag gatgcaagta tagctgcttc atatatacaa cttcaagctc atgaactagg
     6061 tttaggttct tgttggatac aagtaagaaa tagaatgcat gatgataata tagaagctga
     6121 taagtacatt agagaagaat taaaagtacc tagcaagtac agtgtagaat gtataatttc
     6181 tataggatat cctgatgaag aaaagaaagc atacagtgac tctgatttag actatgagaa
     6241 ggttcatttt aacaactttt agtatataat atgctgtatt aagacattat atttttttct
     6301 attgttaata tatacttaaa attgtaaaaa tatatctgaa ttttactaac ttatctaggg
     6361 gttatgtaca aggattttaa agataaatat agaaattaat gtttataatc gatacataac
     6421 tttagtaaca aaatttaagg aggttaaagt atgggtatat ttaaaacaaa aatggatgag
     6481 gactggaaag taaactacat taaagagttt aatgaaatga gagattctta tgagagtaaa
     6541 cttcaaaaaa aacagtttga ggtagatagt ttgaaatcag aattggatag actaagaagc
     6601 tataaaaata gccttaagcc aaaagaaaaa caaattactg atgatgatat aaataatata
     6661 aagagtttaa gaagggatgg cttaagctat aaagaaattt caaatcaaac aagttggagc
     6721 aaagcaactg taagtagagt attaaatgga ctttacgatt agcatataca tggggtgatt
     6781 taaattggga tgaagtatag ttacaaaaaa tgcataatag attctatttt attgatgttt
     6841 atagttcaaa tattaaggat gattttgaat tatgtacttc taagtcaatt tgaatttact
     6901 cttgaaaatt ttaatattat aaacttaatt tcttttacat tagttggttt atcattaatt
     6961 ttattcttaa aagataattc tttgtataac aaggttagaa atagaaaaat cacagaagct
     7021 tttgaagaaa ataaaaacaa tatacttatt gaaaagtgta agctgatact ttttgttgtg
     7081 gttttatctt tagcaataat tgcaacttat tgtactaagg gatatgtact ttttaatgta
     7141 actatgatga ctttgtctgt attgatagtt ccaatatttg aagaattatt ttttagagaa
     7201 tatatatgga actatctaag taattttata aagtcaaaga gtaaaattat ttgtataaca
     7261 tctatactat ctggaattta taatatagga tatatagata ttataagaaa ttatgtaata
     7321 ttgtataaca attcaagcta cacctttgaa gttgttattt caaaaattat gataggcact
     7381 gtttttggta ttgtacttgg attagttaag tataggtcta gagatgtagg attttgtata
     7441 ctattgagga gtttatttgc tatatttatt aggtagatag tataattagt ttataaggtt
     7501 gttttaaatt taattttaaa tcaaccttat tttatttgtg ttctataa
//
LOCUS       DAEMAE010000179         7521 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEMAE010000179 DAEMAE010000000
VERSION     DAEMAE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.181"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..1761
                     /locus_tag="KPH93_002938"
     CDS             61..1761
                     /locus_tag="KPH93_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689715.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVVEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEGTGTDENTDSKDSENQGNV
                     "
     gene            1931..2548
                     /locus_tag="KPH93_002939"
     CDS             1931..2548
                     /locus_tag="KPH93_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689716.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNISELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2909..4102
                     /locus_tag="KPH93_002940"
     CDS             2909..4102
                     /locus_tag="KPH93_002940"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG1689717.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4120..5412
                     /gene="lysA"
                     /locus_tag="KPH93_002941"
     CDS             4120..5412
                     /gene="lysA"
                     /locus_tag="KPH93_002941"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG1689718.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEV"
     gene            5629..6288
                     /locus_tag="KPH93_002942"
     CDS             5629..6288
                     /locus_tag="KPH93_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689719.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDNTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTSGGSDSGGNSPNSNN
                     Q"
     gene            complement(6444..7484)
                     /locus_tag="KPH93_002943"
     CDS             complement(6444..7484)
                     /locus_tag="KPH93_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1689720.1"
                     /translation="MFIGILFFKFINTPSDFISSIEGFLKFFSPFLLAILLALLLNPL
                     VMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLNRLINEMPMYTDYIDS
                     FIEKNMSNIDFLKTLIPHIQHSLDNLLKEASKFIGKIPKNFLIYTLSISSMLFNMTMG
                     FILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHDIFYDYLLGRILDSLI
                     IGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPPIAMTILYSPQKTIWV
                     IVFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFGFIGVFLSVPIAAVVK
                     TYVDKYIDNRLQ"
BASE COUNT         3027 a          810 c         1336 g         2348 t
ORIGIN      
        1 attttttgta aatatatgga tataatagta ataataacat tttttagaga ggagtattaa
       61 atgaaacgat atcttaaaac atatggggtt gcattaataa ttgtaataat ttatgcattt
      121 attaaattac ctgtactaag attagatttt acatctttat tttcagtagg aataattttc
      181 tttgttgtag caggaatact agatatgatg ttagatagaa atgaaagagc atctaaaatg
      241 gctaaatata atttttcaat agcaatagct ttgcttgtgt atattgtagt agttcccttt
      301 ataacttcta cacctgtact tcatgcaaaa tcatatagag aactattggg aaaagtaacc
      361 gaaagtaaat ttactgatga cattagcccc gtcagtgtaa atgacattcg tcttgtagat
      421 gaagatatgg caatgaaatt gggtgacaaa aagcttggag aagttcctgc aatagggagt
      481 gtatccaaat taggaaaatt tcatatacaa aatgtagatg gagagttgta ttgggtagca
      541 cctcttgttc acagagacat aattaaatgg ataacatctc taagtggtac aagtggttat
      601 gtaaaagtat cagctagtaa tcctcaagac gtccaattag ttcaagaaat agatggaaag
      661 ccaataaaga tagtttacca accagaagcc tatttacatc aagatttaca aagacattta
      721 tatatacatg gtattgtgaa tgttggtatg acagatttca ctcttgaaat aaatgatgaa
      781 ggaagacctt attgggttgt aagtttatat gaacataaaa ttggctatgg aggagcaaat
      841 gccacaggaa tagctactgt tgatgcagaa actggaaaaa taaatgttta tgatgttaag
      901 aacacaccta aatgggtaga tagaatacaa ccacaaagct ttgtaacaga ccagattaaa
      961 gactggggtg tttatgttaa tggattttta aattctgtta tatcagaaaa gggagtttta
     1021 gtaccaacag aaggtacatc attagtttat ggaaatgata ataggtctta ttggtataca
     1081 ggtataacat cttcaggtgg agatgaatct acaattggat ttatgttagt cgattctaga
     1141 acaaaagagg ctagattata taaacaacca ggagctactg aaacggctgc aatgaaatcg
     1201 gctgaaggta atgttcaaga aaaaaattat gaagctacct tccctgtaat gtataatata
     1261 ttgggtcagc caacatatgt atcatcatta aaagataagg ctggtcttgt taagagagtt
     1321 gcatttgtat ctgttgaaga ttataatgta cttggaatag gtgaagataa gaatgaagca
     1381 ttaagaaact ataaggatgc attagaatct aagggtaatg atttaaaact tgataatgat
     1441 ttaaaagatg aggtagtaga aggcacagtg acaagaataa gtccagatgt gcgaggtggt
     1501 aacactaatt attatgtaac tttagattca aataaagata taatatttag ggctacttca
     1561 aaagtatcta gcgaattgcc attgactcag gttggtgata aagtcaaaat atcgtattca
     1621 aaagaagaat ctggtgtaat agaaatgtca gaatttgata acttaaatat tgcaaacttt
     1681 gaagataaaa aagcaaaaga aggaacagga actgatgaaa atacagattc caaagattct
     1741 gaaaatcaag gtaatgtata atttaaataa aaagcttgat aacagtattt agcatagtga
     1801 tagctaattt ttatagaatt taattctata aaaattagct attttatttt aattctttgt
     1861 aaatttaatg tcataaaatt aatataatat tagtacaact ttgcaaataa tacttcaagg
     1921 agaatgtact atgacgaata tggaaaaaga gatattactt ttaattagca gggttagatg
     1981 tgttacagag tctcaattta gaaaaattta tggagaccaa aaaagataca ataaaaaaaa
     2041 tttcaaaaaa actttgagaa aaatgtgtag tgaatataca ctaaaaaaat atccttgtga
     2101 tgtggattat tataattata aagacctctc ttatgtatac tatttaaacg gtagtaaact
     2161 ttttaaggga gatgatttaa taaaggctct agtaggatca gaattagttg ttagaatcaa
     2221 tactgcaggt tgtgatgtaa aaagatttta ccgaaatgtt agtgtagatg atgtgaaata
     2281 cgatttattt attgaatata cagaccaatt tggtaatata aagcaagttt tagttgatgt
     2341 tgacttaaat aatgaaatag acatttctaa atacgaaaat ctttctaaaa aaatagataa
     2401 atctacaatt ccatttttta aagtaccaaa cgttttggta gttacaccag ataatatatc
     2461 tgaattaaat gatttagaga gatatgataa tgagtatagc ataaatttta tagatttagg
     2521 attaaataaa ctatttaatt gtatataagc taactttaga aaagaaaact atcggtttgc
     2581 cacattttgt aaaataattg ttgcaatatt atatagaata tgataaaact atattaataa
     2641 attaattaaa aataatgagc ctagggtaga ggtagctgta ttcaatagta ttcaatagga
     2701 gctggtaagc gttgataatt gatgaaaggg tatatagccg aagaaatttt gttgacggac
     2761 aaattttctg gacttatgca gaatatgcat aaggctgtca cttttaaagt ggtgagctac
     2821 aaggttactt ggtctatgaa atttacacgt ccaaagtata cctttgggcg tttttattta
     2881 tcaaaattta gcttgagggg gagaaagcat gagtataatt gtccaaaaat atggtggaag
     2941 ttcagtagca gatactgaaa aaataaaatc aatagcagaa aatataatag aacgaagaaa
     3001 agaaaatcca caaatggtaa tagttgtgtc tgccatggga aaatccactg atgaatatat
     3061 aactttggca aaggaattga gtaatgaacc tagtaagaga gaattagatg ctttaatgtc
     3121 tactggtgaa atgatttcag catctttatt atcaatagca ttaaatgcac ttggatgtaa
     3181 agctataagt tataatgctt atcagttaaa tataaaaacg agtggtcttc atggaaagtc
     3241 tcaaattgat gatataaacg taaacagaat taaaaatagt ttagatgaag gaaatgtcgt
     3301 tatagtaact ggatttcagg gaatcaacga agatggtgat gtaaccactc ttggaagagg
     3361 tggctcagat acatctgctg tagctcttgc ggttaaatta aatggaaaat gtgagatata
     3421 tacagatgtt gatggaattt attttacaga cccaagaaaa tattcaaagg ctagtaaact
     3481 tgatgaaatt gaatatgaag aaatgcttga gttagcgagt ttaggagcac aagtaatgca
     3541 ttcaagaagc atagagcttg cacaaaaata tggtgttgaa atctatgtcg gacgtacatg
     3601 tggtacagaa aaaggaacat atataagagg gggaaaagac atgaaattag aggataaagt
     3661 aataacagga ttagctacta gtgatgatga cagttctata acaataaaag attttaaagc
     3721 tgaaaatata tctagcttat ttgaagatat agcaactata ggtataagtg tagatatgat
     3781 aagccaaact gcacctattt tagataagat aagtgtatca tttacagttc caaaagaaga
     3841 gttgggagaa tgtaaaaaga tagtatctaa atatacagat gaagaacatg tagtgattga
     3901 taataatatc actaaatttt ctttagtagg tcttggtatg aaaaatactt ctggagttgc
     3961 tgctaaagta tttaagatat tcaatgaaaa tggtataatg ataaaactta taactacatc
     4021 agaaattcgt ataacttgtg ctataaattc tgatgataag caagtagcta tagaaaaaat
     4081 agcagaagta tttaatatat aaaatttggg gaggctatta tgaaacttca tggaactatg
     4141 actgtaaata atgatgaatt atatattgga ggagtgagtt gtttagaact tagtaaaaaa
     4201 tatcaaactc cactttatgt atttgatgaa gctttagtta gacaaaattg tagggaatat
     4261 atagaatatt ttaaggctag agagggaaaa aataaggttg cttatgcagg gaaagctttt
     4321 ttacctcttt acatgtgtaa tcttataaat gaagaacaaa tgtgtttaga tgtagtttca
     4381 ggaggcgagt tatatacagc ttataaatca aattttccta tggaaaaagt tttctttcat
     4441 ggaaacaata agactaaaga agaaataaac atgggattag atttaggtgt aggtaaattt
     4501 gtagtggata atttttatga attagatttg cttgaagcat tatgtgaaga taaaggtaaa
     4561 actcaagaga tatactttag aataactcct ggtatagaag ctcatacaca tgattatata
     4621 aaaactggac agatagattc taaatttgga tttgctttag taaatggaga tttatttaga
     4681 gcaatagaaa aattaagtga atataaaaat attaaacttg tgggtcttca tgctcatata
     4741 ggttcacaaa tatttgatgt agaaccatac atagatacag tggatataat gatgaacttg
     4801 gtaaaagaaa taaaagaaaa attcaacata caactaacag aaattgacct aggtggagga
     4861 gttggagttt attatagcga aggagataaa cctaaaacta taaaagagtt ttgtgaaact
     4921 ataatcacta aagcagaaac ttcatgtaaa gagcttggaa tagaagtacc tacgttggta
     4981 atagaaccag gaaggtcaat agttgcaaat gcaggaagta cattgtatac agtaggctct
     5041 ataaaagata taaaagatgt tagggtttat gtaagtgtag atggaggtat gacagataat
     5101 gtaagacctt cgttgtacca agctagttat gagtgtagta ttgtgaataa aattaatcat
     5161 gaggctatga atcatgtaac aatagctgga aaatgttgtg agagtggaga tatattaatt
     5221 ggagatataa acataatgga tattaaaagt ggagatatac ttataacaac atcaactggt
     5281 gcatatggat attcaatgtc ttcaaattat aataaaatac caagagcagc agtagtatca
     5341 gtatttgatg gaaatgataa gataatttgt aagagacaaa gctatgagga cttattagct
     5401 ttagaggtat aaaatggaga agtatataat aaaaattaaa tattaaagtt tattatgagc
     5461 agatttgcta ggatatgtat ctctaaatta atttttggtt tattgtatcg ttcgtagcat
     5521 atctgcttat tttttgtttt actaacaaat ttcttgtaat aaatataaaa tattttaaaa
     5581 actttataat tatgataaaa tttaaagtat atgacaagga gggatttaat gagaggaaga
     5641 gtaattttat taatatcgat attatctata tttcttgtag gttgttcatt taataaaaaa
     5701 gatgaaataa atttagtaga gcaaggaaaa acatatttag aaaaacataa ctataaaaaa
     5761 gctatggaat cattatcctc tgcactggaa gaagataata ctaatgaaaa tgcaagagct
     5821 atgtatatgc aagctatgag aatgtcaaat atgacggagt ttgaagaatt aaaaaattat
     5881 gagggtgcaa tcaaggaact taaacttata gagaaaatca aaaatggttc ctctgttatt
     5941 aaagaagagg caactaaaaa gaaagaagag ttaactaaat tagaaaaaga acaaaacgaa
     6001 gcagaggaac aaagaaagaa aaaggcaaaa gatacggctg gagaagatag gtatagagtt
     6061 gaatcagatg caaggaaatc ttcttatagt ggaagtagta aaaataataa atctagttct
     6121 aaaaataatg ataaaaagtc agatagtaat aagtctgaca ataataacag ttcttctcaa
     6181 aatcaagaaa agccaaataa cagtaatcca acaccagcac caactccaca agatactagt
     6241 ggtggttcag atagtggtgg aaattctcca aactcaaata atcaataagt ataaaatata
     6301 tacataaatt taatgaacaa aatatatatt ataaaaaata tctcttagca attttttctt
     6361 actaagagat atttttttga gaaataattt aataaataac agttaaattt taaactaaaa
     6421 attaaagtca cagtagagat aagctattgt aaacgattat ctatatattt atcaacataa
     6481 gtttttacaa cagcagctat tggtactgaa agaaaaactc ctataaaacc aaatagagat
     6541 tgacctacca aaatagcaga tattacccat atagctccta taccaacttg attacccata
     6601 acttttggtt ctataaaatt tccatctaat tgttgcaata taaatagaaa tacaatgacc
     6661 catatagttt tttgtggact atataatata gtcatggcta ttggagggat tgcaccaata
     6721 aaaggaccaa aatagggaat tatattacct aagaatatta ttgaagctaa aaataaagca
     6781 ttttctattc tgataacaaa ttgaaatcct aaaaatgcga ttataccaat aataagagag
     6841 tctaatattc taccaagcaa ataatcatag aaaatatcat gagtagtcct aaaaaattct
     6901 ataatgttat ctgccttgtt tctagcagta gaagaatata gaaatctctt aaatcccaat
     6961 gcaatttttt ctttgtcata tataatgtat atagatagta taaatcccat tgtcatatta
     7021 aatagcatag aagatatact aagtgtatag attaagaagt ttttaggaat tttaccaata
     7081 aatttacttg cttcttttaa taaattatct aaactatgtt gtatatgagg aattaaagtt
     7141 tttagaaaat ctatgtttga catatttttt tctataaaac tatctatata atcagtatac
     7201 ataggcattt catttatcaa tctatttaat gtattagcta ttgatggaat taataaccta
     7261 atggcaaagg ctaaaataaa acctataaat atataagata aaaaaatggc taaaagtcta
     7321 tgtgctttga actttacctc aaaaagcatc acaagtggat ttaaaagtaa agcaagtaaa
     7381 atagctaata aaaatgggct aaaaaacttt aagaatcctt caatactaga tataaagtct
     7441 gatggagtat taataaattt aaaaaataaa attccaataa acacgactat taaataatat
     7501 tttatatttt cctttgaaat a
//
LOCUS       DAEMAE010000180         7490 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEMAE010000180 DAEMAE010000000
VERSION     DAEMAE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.182"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..1429
                     /gene="cdeA"
                     /locus_tag="KPH93_002944"
     CDS             104..1429
                     /gene="cdeA"
                     /locus_tag="KPH93_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG1689721.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SNALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISMLLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNIVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNLTGLWLTVPFAEVLTLFISILFIKKYKGRYKY"
     gene            complement(1524..2318)
                     /locus_tag="KPH93_002945"
     CDS             complement(1524..2318)
                     /locus_tag="KPH93_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689722.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKILGEAS
                     KTNLSTTVNPILTYITILPFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEVMTGYSIELSVLLKRDMIWTSISALIDPSTSSTPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILIVIYMVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2319..3194)
                     /locus_tag="KPH93_002946"
     CDS             complement(2319..3194)
                     /locus_tag="KPH93_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689723.1"
                     /translation="MSSSIKVENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEKLCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILHEGKIIIDEEIDIIK
                     QKAHYITGNKEELEKLECIKNMKPKKAFGNTVAYFYYGDFSENDEKILKNSTIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3198..3566)
                     /locus_tag="KPH93_002947"
     CDS             complement(3198..3566)
                     /locus_tag="KPH93_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1689724.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3748..4407)
                     /gene="deoC"
                     /locus_tag="KPH93_002948"
     CDS             complement(3748..4407)
                     /gene="deoC"
                     /locus_tag="KPH93_002948"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG1689725.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKVKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNENTTD
                     Y"
     gene            complement(4443..5987)
                     /gene="murJ"
                     /locus_tag="KPH93_002949"
     CDS             complement(4443..5987)
                     /gene="murJ"
                     /locus_tag="KPH93_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG1689726.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKDNFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVIASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGVYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6461..7345)
                     /locus_tag="KPH93_002950"
     CDS             complement(6461..7345)
                     /locus_tag="KPH93_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1689727.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
BASE COUNT         2489 a         1209 c          794 g         2998 t
ORIGIN      
        1 ttgttgattt taagctgttt atcgtataaa taaaattttt cttgactata tagttactat
       61 atagcttatt atatatagag agtaataaaa ggaggtaaaa tttatggaaa atttatttac
      121 aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta tatccatgat
      181 atttatatct ttgtacacaa taatagatgg tatctttgta tcgacattag ttggttccaa
      241 tgctcttgct agtataaata ttgtactacc cataattaac cttgtttgtg gatttggaat
      301 aatgatggca actggtggag gagctatcgt ttctatacgt atgggtgaaa atagacagga
      361 tgaagccaac tctacatttt cttttatagt tttgttttca ttgattgttg ggattttatt
      421 cacagtaatc tcatatttct ttatcaaaga aatatctatg ttacttggtg caacagataa
      481 gttattacca tattgtataa cttatggtaa agttatgatt ttatgtacac cattttatat
      541 tttaaaattt atatttgagt attttgcaag aactgatgga aattctaaat ttagtttatt
      601 tctatcagtc attggtggtg taacaaatat aatattggat tatgtattta ttaaatattt
      661 tggaatgggt cttttaggag ctgcagttgc aactgctata ggtattattt taacttgtgt
      721 tttaggtatt atttacttct tatctaataa atctacacta aaattaagaa aaccaaaaac
      781 tgattttaga cttataagag atactatgat aaatggttct tctgaaatgg ttacagaatt
      841 atctacagga attacaacat tcttatttaa tatagtagct ttaaaattag caggagaaaa
      901 tggacttgct gctcttacta tagtattgta cgctcatttt ttaatgacat cagtctatct
      961 aggattcgct gctggagtgt ctccattaat aagctataat tttggtgctg aaaacagtga
     1021 taaattaaaa gaaacattta aacattctct aaaatttata tttgtttctt ctcttttagt
     1081 gtttattatt gctttagtat ttgcaccatt tattgttagg gtctttgtaa atccagataa
     1141 tacagtattt aaactagcct tacaaggatt aaaaatattt gcatttgctt ttttatttgt
     1201 tggtataaat atatttgcat caggattttt tacagcattt cacaatggaa aaatttcagc
     1261 tattatatct tttagtcgtg cctttgtttt tataatcata ggaatcataa ttcttcctcc
     1321 tatgttaaac ctgactggat tatggcttac agttccattt gctgaggttc taaccctatt
     1381 tatatctatt ctatttataa aaaaatataa aggtagatat aagtattaaa aattaatatt
     1441 taaaatcaac atttactaaa actaataaac ctgctttaaa atacaaattt ataaatttga
     1501 tttttaagca ggttttattt tagttataat aagtcatttg catactcttt aattggtgct
     1561 tttctaagta ataaaattat aaatattatg caaataacta taattcctac ttttgttcca
     1621 aataaaataa aaggattctg aaatgaataa ataatttttt ctaaatacaa tagcatttta
     1681 tgcatatttt tagttgcaaa attaaccata taaattacta ttaatatcgg taatcctatc
     1741 aatatagtaa tactagtaac ttttctaact ctatatgtaa aagctccaag taatactcct
     1801 ataaagccta tacatatttc atttatacat atttgtacaa aataatttaa ataagttagc
     1861 ggtgtacttg atgttgaagg atctatcagt gctgatatag atgtccaaat catatctctc
     1921 tttagtaaaa ctgatagttc tatactgtaa cctgtcataa cttctataaa tagctttgat
     1981 aaaaaaatca aaactatacc taaaatagaa atcacaaagc ttaaaataat taaatctatg
     2041 acaatagctt ttatacaact ttttctatct gctcttatac ttaaagcccc tgaaaagtct
     2101 ttatttgcta tatttactcc atacacaaat ggcagtatag ttatataggt taaaatagga
     2161 ttcacagttg tagataaatt agttttagat gcctctccca aaattttgag gataaacact
     2221 ggcaaaatat tcataacaat tcccacaatc ataaatataa ttatatacaa tttagtattt
     2281 ttattaaaaa attttatata tggctttaat tcattcaatt acaattcctc cttcttagtc
     2341 atgttaacaa acatatcttg tagccctatg tatcctacat caatagttga atttttaagt
     2401 attttttcat cattttcact aaaatcacca tagtaaaaat aagcaactgt atttccaaat
     2461 gcctttttag gtttcatatt ttttatacat tctaattttt ctaactcttc tttatttcca
     2521 gtaatataat gagctttttg ttttatgata tcaatttctt catctataat aattttcccc
     2581 tcatgcaata ttattacatg ctccagtaaa tcatctattt catcaatcaa atgagttgat
     2641 attattattg tccttggatt ttttatataa ctatctaaaa ttatgttata aaactcttgt
     2701 ctatttactg catccaaacc aattgttggc tcatcaaata tggttatagc tgaattagaa
     2761 caaataccta ttatatttga aagtatagtt ttcatacctc ttgacagttg tttatacttt
     2821 tttttagtat ttaaatcaaa atatttgcat aatttttctt ctaagccttt atcataactt
     2881 ttataaaaac tagaagaaaa ttcaaatatc tgacctactt taaaatctgg actataaaat
     2941 tctttttccc tgacaataca aacttcttcc aaaactttta tatcttcttt tgggttcttt
     3001 cctaatattt ctatctcacc ttcattttga attaactgat ttaccattat atttaataat
     3061 gttgtttttc ctgctccatt cttacctaat aaaccatata tcttattttt ttcaaagttt
     3121 agagacatat tattaaaaat attttgcttc ttaaaactgt gagataaatt ttctacctta
     3181 atagaactac tcatatatta atcctccttg aaattaatta taatatcaac caattcttct
     3241 ctatttattt ctaatttgtt agcttcttga agtagatttt ttacaaaatt atgtttaaaa
     3301 ttttcttttc tagcttcttt tataatagtt tttgcaccat ctgatacaaa catccctatc
     3361 cccctcttct tatataaaat atttttatca accaatatat ttataccttt taatactgta
     3421 gctggattaa ttttataaag ctttgaaagc tctgttgttg atggaacctg ttcttcttct
     3481 tttattccat ttgatagtat ctcatcttca attgctcgtg ctatctgaat aaatataggt
     3541 tcttcattat ttaatactaa tttcataggt tcctcctttc tccattagtt agttactcaa
     3601 ctaattaact atataactta taatatcttg aaaaatattt tctgtcaata ctaaataaaa
     3661 aacagaggga aaattccctc tgttttttgt tctttatatt tataattaaa ttttagtttt
     3721 tgtaatccta tccataagtt aaaaacctta ataatctgtt gtattttcat tttttaatat
     3781 tttacatatt gagctagttc ctaacctttc tgctcctgcc tctataaatt tttctgcatc
     3841 agaaattgat ttaactcccc ctgcagcttt tatttttaca ttttttccaa catgctcttt
     3901 cataagcttt atatcttcta aagtagctcc acctgttccc attccagtag atgtttttat
     3961 aaaatctgaa cctgacattg ttactatttc acacattttt actttttcat cttcatctaa
     4021 ataacatgtc tctataatga cttttaatac tttatctcct atagcttttt ttatttcttt
     4081 tatttctttt cctatattat cataatcttt attttttata tctgaaatgt ttacaaccat
     4141 atcaacttca tctgctccat tttcaatagc atcttttgct tcaaatactt ttgtagcagt
     4201 agtttgatat cccaaaggga accctatcac tgtacatatc tttattttac cttttagata
     4261 ttctgatgct cttttcacat atgatggtgg tatgcaaact gatgctacac tcatatctac
     4321 agcttcatca cataaaattt taatatcttc ccatgtagtt gtagctttta aaatagtatg
     4381 gtctactgtt tttaatatat gtttcataaa atcctcctaa gtttataatt aatataatta
     4441 gattactttt ttaaatgcaa ttttctcttt gcaatgttta gtatcatatc aacctcttca
     4501 actttaaaaa tagtcattag tagtgtataa actccacctc caactataac tgaaatagct
     4561 agtgatacaa actcattaaa tgttcctact cccaaaattc caaatataaa tttatacgta
     4621 aagtatgata aaacacccat aatcaaagat gcaattaccg atttcaaagt tgcttttatt
     4681 attttatctt gtccaaaata tccaactttt ctctttaggt tcaagaagag taacaatata
     4741 catgcaattg atgatgaact agttgcaagt gcaaggccac catgagccat tggttttatt
     4801 aaaactaagt ccagtactat atttacccct acagatataa tcccatttac cataggtgtt
     4861 tttgtgtctt gtaatgaata aaaaaccttt cccaaaatat ctcttaaacc aaatgcagtc
     4921 ataccaactg cataaaaaat taaggcagtt gctgtcattt gggttgccct agcatcaaaa
     4981 gcacctctct caaaaatgat tctaacaaca ggagttgcaa aaaaaataga agcaactgaa
     5041 ataggaatca tagaaattat tatcatattt atactagatt taactgaact agtaaacttc
     5101 ttttgattat tttctgcact taacttagat aacataggat acataacaga tactattgaa
     5161 gctgtaaatg tccctgtaac aaaaccattt aatctatccg cataagtaag tgctggaatg
     5221 cttcctttaa ctaatgttga ggctaaagtt gtatctacca atgagttcac ttgattaaca
     5281 gcaacaccta taaatactgg tgaaagtaat gctagtaatt taataaggct atcatcttta
     5341 aagtttaagt agtataagta tttgtagttc gtttttttaa caaaaaacat gtaaaaaagc
     5401 agttgaacca ccatagcaac cacagcacca attggaagta tgtatggtcc aaatacggta
     5461 cttaacatta ttgaaataat tattactata ttatagggta tacttccaaa tccaactaca
     5521 ataaagttat ctttaatttg taaaaatgct gacataacac tcgtaatacc tatgaatatt
     5581 attcctgtta tcaatatttt agtaaatttt acggtaagta aaaacctctc tccttcaaat
     5641 cctatagcaa atatacttac aagttgcttt gagaaaataa ctcctaatat agctacaact
     5701 atgcatatac caactattat atttaaaaca ttgtttaaaa actttagtgc ttgcttttca
     5761 ccttgtttac tacttatatc ttgatacatt ggaataaaag tagtaactat agcagtacct
     5821 attgctgcaa atataatatt tggtatattc atagctgtta aataactttc tgtatataag
     5881 cctgtgccat aaattgatga taatactaat tctctaccca ttccaagaat cttagagatt
     5941 atagtaacta tcatcaaata aaaagttgct ttagctacct tgctcatttt atcactccac
     6001 ttctaacata cctcttttta atcaagtaaa atttggctca ttagatataa ttctatcaac
     6061 taatttgcct tgcttagctc tatcaaaata taattaacac ctatttctta atatatcaca
     6121 aactacatta ttcttcatat ttttttattt ctccagtaag ttttacatct ttcccatata
     6181 aattgtatag cttcaattat attcaaagaa atggagttgc tacttgataa ctttaaaagt
     6241 aaaaaacaga ttttattatc tttttaaact atattctaac atattttgtc catattgaga
     6301 aattttacta aactattatg tattttatta aataaataaa ataacagcac acctataaaa
     6361 tacaagtgtg ctgttcataa acagatttta taaaaaaata attcctaagt agagattaac
     6421 atgctttctc atactctcat atataatgtg tttaaaaatg ttaataaaaa tgctttaatt
     6481 gttttaactt acttggatgt cttaattttc ttattgcttt agcttctatc tgtcttattc
     6541 gttctctggt aactccaaat acctttccaa tctgttctag tgttttaggg tcatcatcat
     6601 caattccaaa tctcattctt aaaacttgct gttcttgctc tccaagacct gtaagtagtt
     6661 cttctatctt ttcttttaaa ttatgctgtt caacttcgtg tattacaaca tctttaaaat
     6721 cagcatctga tggtataaat tcaactaaac tactatcctc atcttctttt aatggagtat
     6781 ccaaagatac aacattttta tctcttctta ataattccaa cactttgtca acttctaagc
     6841 cacatgcatc tgcaatctca tctatagttg gctctcttaa taatacattt aaaagttctt
     6901 gtttcttttt ctttacaaaa tttattctct gatgaagatg tataggtatc ctaatagtat
     6961 tttcgcaatc gtcgatatat cttgttatac tttgttttat ccaccaagtt gcataagtgc
     7021 taaatttata tcctttttta taatcgtact tttctacagc ttttataagt cctatatttc
     7081 ctgcttgaat taaatccaac atatcaatac tatctctttt atatctcttt gctatgctta
     7141 ctaccagcct ataatttgaa tttataaatt tttcttttgc tactgaagat gagttgatta
     7201 tttccttagc cagctctacc tcttctcctg ctgacaacat tttgtattcc tctatttcct
     7261 ttaaatacat tttcatcgca tttgatacat ttgatgaacc cacacaaata taattttcat
     7321 aattattttc ttttttagta tccataatca aaccccttct attcatagaa tttgctaaaa
     7381 caaaaataaa taatttaaac ctacataatc tacaattaca taatatccca ttctttaaat
     7441 gaattgtttt gtatattatg tatacaaatt tataaaaact atacctataa
//
LOCUS       DAEMAE010000181         7465 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEMAE010000181 DAEMAE010000000
VERSION     DAEMAE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.183"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            235..331
                     /locus_tag="KPH93_002951"
     tRNA            235..331
                     /locus_tag="KPH93_002951"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:269..271,aa:Sec,seq:tca)
     gene            482..1189
                     /locus_tag="KPH93_002952"
     CDS             482..1189
                     /locus_tag="KPH93_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG1689728.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(1331..2167)
                     /locus_tag="KPH93_002953"
     CDS             complement(1331..2167)
                     /locus_tag="KPH93_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1689729.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHRKVIDNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            2355..3260
                     /locus_tag="KPH93_002954"
     CDS             2355..3260
                     /locus_tag="KPH93_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG1689730.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRHEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            3269..4453
                     /locus_tag="KPH93_002955"
     CDS             3269..4453
                     /locus_tag="KPH93_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689731.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSDLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEGKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RIINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            4568..5869
                     /locus_tag="KPH93_002956"
     CDS             4568..5869
                     /locus_tag="KPH93_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG1689732.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGKIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSILIESIFFLLEKISNLSFAYTNIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            5823..6866
                     /gene="holA"
                     /locus_tag="KPH93_002957"
     CDS             5823..6866
                     /gene="holA"
                     /locus_tag="KPH93_002957"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG1689733.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(7053..7319)
                     /gene="rpsT"
                     /locus_tag="KPH93_002958"
     CDS             complement(7053..7319)
                     /gene="rpsT"
                     /locus_tag="KPH93_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG1689734.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         2935 a          710 c         1147 g         2673 t
ORIGIN      
        1 ttatatttgt actggaaatt atattttgat tatatagaga agaaactctt tatagattat
       61 taaaatataa tttccagttt tttattttgg atatgtaata aaacaggtaa attttaacca
      121 tgtatagata aaagctattt aaattataat ttactatcag ataaatctat aaaaattgtt
      181 gcaaagagtc aaaatatatg atattataga tttcgttgag aaggatttca ttatgggagt
      241 ggatgggtgc cggtgtgccc tctagtcttc aaaactagta tgaggggtta agagcctctt
      301 aggtgggttc gattcccacg cattcccgcc aatatgtaaa aggtatcttt taattaaaag
      361 ataccttttt aaatttattt taagataatt tatttttaat aatatataat attttgtatt
      421 atataatgtg aattttctaa tactattata tatttagata aaaatctagg agtgtggaaa
      481 gatggaaaat aattttacaa cgagaactag ttttctagtt ggagatgatg ggatagaaaa
      541 acttaataat tcaaatataa tagtatttgg agttggagga gttggtagtt ttactgttga
      601 agctttagca agagctggag taggcaacat tactattgta gattttgacg atgtagatat
      661 aaccaatata aatagacaaa taccagccct acattcaact gtaggaaggt acaaagttga
      721 tgtcatggaa gaaagaatat tagatataaa tcctaatatc aatataaaaa aaataagaag
      781 tctttataat aaagatacta gtgatgaaat actaactgaa agatatgatt atgttgtgga
      841 tgcgatagac atggtaagtt ctaaaataca tctaattgaa acttgtgaaa aaaaaggatt
      901 gaaaataata agttctatgg gtatgggaaa taaattagac ccaactaaaa tagtcgttac
      961 agatatacac aaaacaagta cttgtcctct agctaaagtt atgagaaaag aacttaggga
     1021 tagaggtatc aaaaagttaa aagtagtata ttctactgaa caaccaatag aacttaagaa
     1081 gaaagtaatg aatggtagaa aagtaacacc tggtagtgta tcatttgtcc catctgtagg
     1141 aggtttaata atcgcatctg taatagtaaa tgaactatta ggacaataaa ttttaaatat
     1201 tttaatttat aaattttata tacaaacttg aaggctagaa gcattaatat aaaacaattt
     1261 tatattcata cttttagcct aattatttta tattacctta ttttatgtag ttgtagtttt
     1321 attttttaga ttatgtatct atttcataat tatattttgt aaatataatt tctccatcac
     1381 tataaaaaac aatatctcct attctatctg ttctataaat tgatacatgg ttttgtttta
     1441 gattatcaat aaccttccta tgtggatgac catattgatt tttatatcca catgatattg
     1501 cagctatatc cggactaatt ttttttataa attctaaaga tgatgaagaa ttacttccat
     1561 gatgacctat ttttataaaa ttaatattat ttaatttaaa agaatgtaaa atttcttttt
     1621 cattttcttc ttcagcatca cccataaata aaaaagaatt atcgttaaaa gttaatttga
     1681 aaactataga atttaaatta gattcttctt gtatatatga tggacttaat acatatatat
     1741 ttatattgtt atctatgttt aaaacatcat ttttatagag atgctgaata gataagtttt
     1801 tatctgtaca agaatttatt agattttgat aagcctcact atcagttgat tgttcaggca
     1861 tatatatgga gtttacatta aattttttta taacattatc aagacttcct atgtggtctg
     1921 aatctggatg tgttgctatt ataatatcaa tagtttttat tcttttttgt ctaagataac
     1981 taataattat attttcgcta tcttcatcac ctccatcaat taatatgttc ttatttgtgg
     2041 gtgtttgaac taaaatacta tcaccttgtc caacatcaat catatgtata gatagtagag
     2101 attttttgtc acagccgatt aaaagtgata ttataataag tattgataaa aaaattttat
     2161 ttctcaataa aaaatcactc cattcattac atgtttatat aaatttatga tgggtaatat
     2221 aagatatata tgacttatta tattttatat tttaaacaat ataaatgaaa ttaataccat
     2281 aataaaattg gagtttttta tatatacaaa ataaaatttt taaaattaag caataaaaaa
     2341 ggtaggagga ttttatgttt ttcaaagata ttgaactaaa ttcaaaaaaa gagttagacc
     2401 catattttga tttagtagat tatgaagctt gtgagtactg ttttagtact ttatatatgt
     2461 ggcaacatgt ttataagaca ggatattata taggtgaaga ttttgccgtt ttagttgggg
     2521 aatacgaagg agatagcttc tcaatattac cacttgctaa aaaagataaa cttccagaag
     2581 ttgtagattt tgtccttgaa tatttttcta aaaataataa aaaaatatac ttaagaggta
     2641 ttactactga agtagtagag tttttaaaag aaaaatatcc aggtagattt gagtatatag
     2701 aagaaagaga tttatttgac tatatttatg atgcagaaag tttaagaact ctagctggta
     2761 aaaagaatca aaagaagaga aaccatataa actatttctt aaaagagtat gcaggtagac
     2821 atgaagctaa attattagat aaagaaaact ttgatgagtg tttagtacta atgaaggaat
     2881 gggaaagtaa taaggaagaa aataatgaat ttgatgaaag tatggatgat gaattaatag
     2941 gtatcaaaaa gatatttaac cattatgaca tattaaaaga taaggtaaaa gtgtttggtg
     3001 tttatgtgga tggaaaatta gaagctttta gtataggaga acttttaaat ccaaatatgg
     3061 cattgattca tatagaaaaa gctaatcccg atataagagg tctgtatcca tttataaatc
     3121 aacaattttt ggtaagtgaa tttaaggacg ttgagtttgt aaatagagaa gaagatttag
     3181 gtattgaagg attaagaaaa gctaaacttt cttatcatcc ttgcagattt gttgaaaagt
     3241 acagtgttag ggaggcttaa tagactatat ggagattagg tatgctaaag aagaagagat
     3301 agaaagtata aaagagattt ggagttattg ttttaacgat acggagagtt ttatgaagta
     3361 ctattttaat gataaatata aatctgaaaa tactgtagta gctttagatg aaggaaaaat
     3421 tatttcttct cttcagctaa atcagtataa gttattacta aatagcaagg tatataatac
     3481 atcatatgta gtaggagttt ctacacttcc agagggaaga ggagctggat atatgaacaa
     3541 agtaatgaaa tttactttaa acgaattgta taaaaaaggg caattagttt ctatattgat
     3601 gcctattgac tatagacttt atagaagatt tggatatgag cattgttacg accagattga
     3661 atatactatt aacacagatg atttaaaaaa ttttaagtca agtggaaaaa tgataaagtc
     3721 tgatttatca caaattgatg atttgatacg aatagataga acttttttaa atgaggttaa
     3781 tggtaatgtg ttaaaagatg aacattatta tgaaaactta tttaaagaga ttcagagtga
     3841 agatggattt ttatacattc atgaaggaaa tgaaaaagat ggatatatag tttattttct
     3901 tcaggaaggt aaaatgtttg tcagagagtt attttataaa aatatagatg cccttaaatc
     3961 aatgcttaaa tttatatata atcacaatac tcaatgtaag atagtcacta tatctactcc
     4021 tacaatagat aagataagat ttatattaga caaccctaaa gatagtgata taaaaataaa
     4081 accttttatg atgggaagaa ttataaatgt aaagaagttt attgaagata tagatattga
     4141 gaaggatatt aatagctcat ttaatttatt aatagaagac aagtttatag atgaaaataa
     4201 tggtctattt aaaatttcta tacagaataa gaaggtatct gtagagcagt tagataaaaa
     4261 aggtgcagaa aagcctcaag aagattttga cattaaatta gatataaata ccttaacaca
     4321 gttatcattt tcttatatag atgtaaatga agccattttc ttaaatgata taaaagatgt
     4381 ttcagaagaa acactagaaa cacttaattg tattttttct aaaaaaaata cctatataaa
     4441 tgaatatata taggtagagt acgttaaaag attttaaaaa tcttttgacg tatttttttg
     4501 tttagataac aatttatctt gtttaaaaat tttctatttg aaaataatct aaataatata
     4561 cttttatatg ggagtgatta taattcaaat aagaagacct ttattaatta tattaatgtt
     4621 tgtggtagct atatcattta ttattacaaa taacacaaaa aacgacgaag aacttaatga
     4681 taagataata actatacaag gagttgtaaa aggaaaaatt gaaaagaaaa gatacaatca
     4741 gtataaggta ggagtttttc taataaatga ttatacaaaa aataaaaatt tgaagattgg
     4801 tcagaaggtt aatattacag gtaaatttaa aagtttagat aaaatgaagt atgatgattt
     4861 tgattatgga aggtatataa aaagcactgg atataaaggt atagtgtata taaataacta
     4921 taaaattatt gataaaaata aactttactc tttaataggg aaaataaagt tttatattag
     4981 taagacttat aggtacttat ataagaaaaa ctcagatttt ataaattcta tattattagc
     5041 agaagttgaa aatttgactg aggaacaaaa agaaattttt accagaacgg gaactagcca
     5101 tgtaatatct atatcaggtc ttcatacagg aatattatgt gtaattatat cctttctatt
     5161 aagaggtata aataaattgt ataaattatt aatactgtcg atttttataa ctttatattg
     5221 tataatggta ggtgcgtctc catccataat taggtctata gcatttgtta tgatttttta
     5281 tttatcaata tttattgata gaaagaaaga tggaatatca gcattgtctc taattgggat
     5341 aattttaatt atgaataacc cctatgttat atataatgta agttttcaat tgtctttttt
     5401 agctacactt tctatattaa tattctataa taaaataaat agtattataa aattaagtat
     5461 ggtatcattg acaatatcat caaatatatt aactttacct ataatttatt atacatttaa
     5521 aggtatacca ttgctttcaa ttataggaaa tttaattata gtaccatttg tgggagtaat
     5581 aatgtattta agtattgcaa gtttaatagt atttaaagta agtgtagtaa tagctaaaat
     5641 tatatctttt tttaatagca tattgataga aagcatattt tttttactag aaaaaatcag
     5701 caatttaagt tttgcataca ctaatataga aagtcctaaa ttttatattg ttgttattta
     5761 ttacatagga gtgttttttt acatatttta catagaagga aaagagataa aggagcaaga
     5821 gaatgaatta caaggatatt ataaggaatg taaaagagaa aaactttgaa aaaatgtatc
     5881 ttttttatgg aagagagtat tatttaattg agaatgcaat aaaagcattt aaagatagtc
     5941 taaacgaggg aatgcttgat tttaatttag atataataga tgggaaggaa atagtcttaa
     6001 atcagcttat aagctctata gaaacacttc catttatgga tgatagaaaa atagtaataa
     6061 taaaagactt tgaactatta aaaggaaaga aaaagaattt tacagatagt gatgagaaat
     6121 acttaataga gcatttagac aatattccag atactacaac tatagttttt gttgtatatg
     6181 gagatgtaga caaaagaaaa tcattagtta agaaaatagg gaacaatgga attgtttttg
     6241 attgtgataa gctttctgat atggatttat ttaaatggat aaaaaaatct tttgctctaa
     6301 atgatgttat aatagataat tctcaaatca tgtattttat tgaacaagaa ggatatagag
     6361 ataagagtag tgaaaagaca ttatctgatt tagaaaatga aataaataaa ataagttcct
     6421 ttgttggtaa agggaataat gtaaccaatg atgtaattga taaattatct caaaaaaaag
     6481 ttgagaatga tatattcaaa ttaatcgatt atattggaga acaaaatgct tctaatgcaa
     6541 tgaaaatatt aaatgatatg atacaagaag gtgaatctgt attaggtata ttttctatga
     6601 tagcaagaca atttaaaata attatgcaag taagacagtt acaacttgat ggatattcaa
     6661 caaaacttat agctgataaa ttaaaaatgc atcaatttgt cgtaggaaaa gcattaaagc
     6721 aaactaaaaa tttttctgat gatataattg tggaaatatt aaactatata ttagaaagtg
     6781 attacaaaat aaagacaggt cttataagag atactttagc agttgaaatg ttggttagta
     6841 gatactgtaa aagagaagca atttaaaaaa tctatatatt aataaaaaat aaatagcaag
     6901 atatgttata gttcagagta gatgtaagct aaattaagtt tgatatacaa tcaactataa
     6961 tatgtctaac tatttatttt ttattgtata aatttgcaaa ataaaaaacc cctacatagg
     7021 ggttattata tataagaaat ataaactggt aattaagcgt tcataccgtt taatttttga
     7081 gctaatttag ctacttttct agctgcagca ttcttatgta tagttccttt agaagctact
     7141 tggtatattc ttttttgagc gtattggaac ttagcaacag catcctctct gtttccagct
     7201 gcaacagcat cttcgaatct tcttattgca gtttttattt gagattttct aactctattt
     7261 aaagcagttt tcttttcgat aacacttatt cttttcttag ctgattttat atttgccacc
     7321 gtgttcacct ccctgtattt ttttatcata ttcatcgtca acatattaga ttttaacaga
     7381 aataaatcta aaaatcaagg attattattt atatatttta ataatgtttt ttattttgat
     7441 tttttagact gtaaattgtg tataa
//
LOCUS       DAEMAE010000182         7355 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEMAE010000182 DAEMAE010000000
VERSION     DAEMAE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.184"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(381..2447)
                     /locus_tag="KPH93_002959"
     CDS             complement(381..2447)
                     /locus_tag="KPH93_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG1689735.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLISSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWV
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKRFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2524..5553)
                     /locus_tag="KPH93_002960"
     CDS             complement(2524..5553)
                     /locus_tag="KPH93_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1689736.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFLDGLHSNLEQKESYKNIYKIK
                     NKDNECGDGNIYASVQYIDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIMSI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTESKFEEYGYMEK
                     NKFLKKLRGLDKYLQENYETENTYIYKFESKDKKLKWVRMNIFEDELKILGVVVDVTK
                     EFIEKKKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNSIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVEMKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5606..7066)
                     /locus_tag="KPH93_002961"
     CDS             complement(5606..7066)
                     /locus_tag="KPH93_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG1689737.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDLNKINELV
                     KNGTYKKDAVAQFNNDKNFETWFESFKAKLENAIKK"
     gene            complement(7158..>7355)
                     /locus_tag="KPH93_002962"
     CDS             complement(7158..>7355)
                     /locus_tag="KPH93_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689738.1"
                     /translation="VFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVKA
                     LFSIFVIVCFLIAFKYVVNPV"
BASE COUNT         2353 a         1282 c          685 g         3035 t
ORIGIN      
        1 aatactaaat aatattttct acatgcttgc tttttattta ataatactag ataatatctt
       61 tcacatggtt gctttttctt taataatact aaatagtatc tttcacactg ttgcttttta
      121 tttaataata ctaaatagta tctttcacac tgatgccttt tctttaataa tactaaataa
      181 tatctttcac attgttgctt tttctttagt aatactaaat aatatctttc acattgttgc
      241 tttttctaaa caactataaa taatatatta aacctttatt tctcttttct aataagttaa
      301 ataatacatt ttacacatat ctatttgtct tgcacataat aaataataca ttatatttct
      361 atctaaattt agattctaaa ttaaatagta taaattatat tcttcaataa acaagttttc
      421 aaatccaaga ttcgctctct atctttaaac tttattgtaa acataattcc atctttttta
      481 attatttctc cttcatgaaa tcttttatgg tataccttat cccctacacc aatactgtca
      541 atttcttttt tcgttggaga ttttatatca ttaataaata atgatttatc aactttctta
      601 ccatacttat ttaaggtaga gcttatacat aacttttctt cagctcttgt aatagcaaca
      661 tacataagcc ttctttcttc ttctaattgt tcttcttttt tctcttcctt acaaatatca
      721 tatgattttt catgaggtat tgttccttca tttacaccta taatataaac atttctaaat
      781 tctaatcctt ttgcactatg catagttgta aatataacac cctcagtctg cttattcttg
      841 ttattttccg ttaactcagt ctttacccta tcaatatgct ctaaaaactc tgtaacagta
      901 ttaaaattag ttgctgaact ctcaagttca tttaatatct ctacaagtcc attagtcttt
      961 atctttctgt tgctacaata atctaaaacg tatctatcat aatcaagact agttctaata
     1021 taagatattg catttttagg atttaaagta tttaaataac ttaaatctat ttccaaatca
     1081 tttattgttt taacttgttt aggatgaaga ttacacttat ttattaatgc tgttataaag
     1141 tctttttcat ctttaaccat atttatactg tctttggata tatatctaaa aggcttgttt
     1201 ataattctca accaatcttt atttgatttt ggattaatac ctattctcaa ataagctagt
     1261 atatcttgac ttacccaatg gtcatatatt gtaattactg agtcttttac tacaaatgga
     1321 atcctcatat ccataaatac atctactaat gctcttgact gtatattagt tctataaatt
     1381 actgcaaaat ctgaatactc tacataatct ttttgtattt cgtcaattat ttccctggca
     1441 atatataagg cttcttcctc tgaatcatgt ggagaaatat aggtaacact tcctccatcc
     1501 ccctgactac acttaatgac tttttcatat ctatttttat ttttgtcgat aagtctattt
     1561 gcagtatgca ctatctcact tttagacctg taatttatat ctaatattat ttttttagta
     1621 ttattaaagt attgttcaaa ttcaagcaaa aaatctggtc tagcacctct aaatccatat
     1681 atactttggt cttcatctcc aactgcaaat atattgttta atggactaga aattaacttt
     1741 aaaacttcaa actgtacttt gtttatatct tgaaattcat ctatcaatat atatttatac
     1801 acatttctaa ccatttctag tgcagactta ttgttcaaaa gtaaataata agttcttata
     1861 agcatatcat caaaatctat tttgttaact ttagattttt gttcttcgta taagttgtat
     1921 acttttaaaa actcatcttt tgttaatact tctgaattaa attcattttt atccataagt
     1981 tcatttttta cataggaaat ttcatttata acttgtccta tattctcatc atcatcagca
     2041 ttctctatat ttaaactttt taagattgcc ttgattgtca ttcgctttga cttttcatca
     2101 aatatactgt ctaaattgta tctttcaaag tatcttagaa ttttaaaaaa tacagagtgg
     2161 aaagtaccat aggttacctt attaagtctt atatcatcac ttaaattcaa agctctattt
     2221 ttcatttcta ttgaagatgc cttggtaaaa cttattgcca agattcttgt cggtgctatg
     2281 tttttgttta caaccatatt ggctattcta tatgtaatta ctctggtctt acctgaacca
     2341 ggccctgcaa gtatcatgca aggcccatct atatgctcaa ctgcttctaa ttgattttca
     2401 tttaactttc ttatatctat atttgttgtg attattagct ccaccaccct aatttataat
     2461 tatagtattt tctttttaat tataaactaa aagttcataa aaataaattt aatttttcct
     2521 ctattaaacc acatcattcc tattttcatg acatatcttg agctcttttt taattttgtc
     2581 acttatatct tcaggtataa atgatggttt actaatatag taaccttgca tataatctac
     2641 tccaagatta attagtgttt ccatttcacc tttcatttct acaccttctg ctatcacttt
     2701 aatatttctt tcattagaat atgagatgag attttttaat attttctgtc tgttttcatc
     2761 tttatcaata cctcttacaa tagacatatc aatctttaca tagtctggca ttatatataa
     2821 tagtacagca tccccattgt aacctgtccc aaagtcatct aaagcaattt ctatattcca
     2881 atggtctata tatgctttct ttttctcaat aaaatcttca ttaatcttat cattctctgt
     2941 tatttctaat actactctat ttaaatattc accatataaa tcttcaattt cctcaaaatc
     3001 tacttttgat aaattattat taggaataga atttaagaac agtttgcaat cgccaaattt
     3061 ttctttgtat tttttaaatg atttaagtgc tttaaaccat gtaatacgct caatttgata
     3121 taatttagac tgtgaacgag ctaatctaat gacatctatt ggtgaactaa aagtttttat
     3181 tttaggtctc ataagtgctt catatgcaaa tactttacct ttacaatcta caataggctg
     3241 aaatgcatac tcaactagtt ggccatcaat taacttgttt aattcttctc tattactcaa
     3301 taagaatgaa tccttattat atatattaat atcaaactca ctaacttcac ccttaattgt
     3361 atttttaatt tgatacatag caaaatctgc gtatctcatt aattcatcat agttggttga
     3421 atcatcaggg taccatgaaa ttcctgctga tgcccttatt ttaaattctg ttccatcatg
     3481 aagttttaat aaggtattat tcatctgttt cttaacagaa ttaataatat ctcttatact
     3541 atctttactt tcatatccat atataaatac ataaaactca tcacctgaaa gtctagatac
     3601 tatactattc catgatataa acttcttaag tatatctgca gcacacctaa tatattcatc
     3661 accagcatca tgtccatatg tatcattaat atattttaga ttatctaaat cccacataat
     3721 aaatgctgct atcttaagtt catctttatt tttaaatttt tcttgtatct gtgaatgaaa
     3781 agctctccta tttaaaagat tcgttaaaat atcaaaatct ctttcatatt ctatcttctt
     3841 tttctcaata aattcttttg tgacatcaac aacaacacca agaatcttta attcatcttc
     3901 aaagatattc attcttaccc acttaagttt tttatcttta gattcaaact tatatatata
     3961 tgtattttct gtttcataat tttcttgcaa atatttatct agtcccctta attttttaag
     4021 gaacttattt ttttccatat agccatattc ttcaaatttg ctttcagtag taaacacgct
     4081 aaaaaattct tttgtacaaa aaacattctc tgcgtttttg tataactcaa aagctccgat
     4141 tggcatatct accatttcaa taatctgtga taattttgat gaagaatctg caacatttgc
     4201 actgagtaac tctatagctg aagataattc atctatttca gatatattta tcttatcaag
     4261 atttactggt tttgatggat tactatttct aactttgttg gctaaaagaa caattggttt
     4321 tgtaaataat ctcgatgcaa tgtaaatacc tattagacct attaataaag aaattgctat
     4381 agacattata actaatttct ctaccctttt tgaaaatgac aaaagacttt tttcttcaat
     4441 aatacccaaa agagcccaac gttcattttc aaatggagta ttggagttat ataaatctat
     4501 gtattgtaca ctagcataaa tattgccatc accacattca ttatctttat ttttaatctt
     4561 atatatattt ttatacgatt ccttctgctc taaattagaa tgtaagccat ccaaaaattt
     4621 ttcttttaat gaatatccat ttgaaattac attttcaaaa gccatatcat tatttctatc
     4681 tacacctaaa atataattac cttttttatt tccatttatc tctgagtatg gaagtaattc
     4741 tcttaaataa tttacactta aatctattcc aagtactcca tatacagttc catcttcata
     4801 gataagtgga actgtatatg tcataagcct atcacctgca tttgtcaata aaaacttact
     4861 tccccagtat cctaaattag aacttgatac atttggatta tcaattgcag ctctaaatgg
     4921 tttataaaaa aaatcatctc cttcttgctc attttcactt ttaaatgaaa acttaggttc
     4981 ccaataactg tccatagata ttccaaaact tcttgctata tctgatgaac ctctctcaat
     5041 taacaaatca gaattatcat ttggattaaa cttagggtct aaatccctta tatacaatcc
     5101 agtcctactt atttcacctt tactatctat tggcttatcg ttaatatttt tgtcacttag
     5161 gattataaaa gcccctgtaa cagaattttt tcttaaaaga tatattaaat catttgatat
     5221 actttttata atatctttgt taatttcttc attgttgcct acatcctcaa ttaaggcatt
     5281 tttttcattt aatacttttt ttacagagga attaattgta gcttccgttt cacttgtatt
     5341 tgaccatctt tgtaacattt cattttgtat ataattttta cgattaatta ccctttcatt
     5401 taagatgtca aacgaattat catttaattt cttaatagtt cctccccata aaattgtggc
     5461 acaaaataaa cttgtttgaa ttagcataac aataattagg ggaaatatca tctttttcat
     5521 tattgaattg ttttttctta gattgttttc catacttatt ccacctatac ctttgtgctc
     5581 gtcgacacac taactgccaa ttcatctatt tttttatagc attttcaagc ttagccttaa
     5641 aactttcaaa ccaagtttca aaattcttat cattattaaa ttgtgctact gcatccttct
     5701 tataagttcc attttttact aattcattta ttttatttaa atcttcttta gctttatcaa
     5761 gcattgaata ttcaagtatt tgtctagctt tagttccacc atcaaatgct ttattagtat
     5821 ataaattaca actgttaatc tgttcaatag atgaggctat tgaaccttca actttatcag
     5881 ataaactcaa attatctttc ttcataactt cttttataac atccaaatca ttcgtttttt
     5941 tctttactgg taagtatcca gaagcaatag agaattttat attaggttct ttatctgtaa
     6001 accattttaa aaattctact gatgcatatt cttcttcttt agtggatttt gttacagcca
     6061 ttcctgcacc ttgttgtaca gcatattttc cttctccttt aaaacgtggt gcttgaaaaa
     6121 cagatgtttc aatattataa ctctcattat catttagcat tactctatct ggaaagtatg
     6181 ctgctcctga actagaacct acaagagcta atatatcacc tgtttttgca tcatcagaac
     6241 gaaacttacc ataagaagca aaatatccat ttatatatgg tacatagaaa ttatcccata
     6301 gttttttcat aactttttca tcaatattta gtgtaacttt accatcttta acagaaaaaa
     6361 tttctactcc tagttgttta cttcctataa taatataatt tgctaaagca tctcttccaa
     6421 aaaatgcctt accatcatta ggctctttcg ttaaactatc tgtccattca taatattttt
     6481 ttgatgtctc tactaatcct tcaattgttt ttatatcatt tatatttgaa tttgtagctt
     6541 ttgcaaattt atcccaatca gttttattta acatcaaaat ttcagttgat tttgcaattg
     6601 ggaaaagttt aaactcatcc ttagaattta ttctgccttc ttctacatat tcgtctatgt
     6661 attcatttaa ttcttcttta cttatgtact tatccatatc tactagtaac cctaatttat
     6721 ccatttcata tgcagtatct gagtatgctg caaatatatt tggtacatct tcagaaccaa
     6781 cttttttatt tgcagaatct aaaacttttt ctgataaatc aactacatta ccttgactga
     6841 atgcttcaac aataattcct ttttcagcac ctacagtgtc attaaattcc ttaacaagct
     6901 tatcaaaagc cattttttgc tgtccattat aataatgcca aatttgtatt gatgtcggat
     6961 tttctgggtc taacttataa ttatcttcct tctttaaact acatcctgta agtaataaaa
     7021 caatcattga tgtgataatt aattttgcaa acttacacct attcataaaa tctactcctt
     7081 caaattataa aatttataat agcattaaaa ctgtaacttc actatttata ttaattatag
     7141 aacgtttatt gatatattta aacaggattt acaacatatt taaaagcaat taagaaacat
     7201 actattacaa atatagaaaa taaagctttt acataatttt tatttctaaa tgcaccaaat
     7261 gacaccaatc cttgttgaat agctactgta attactaaaa gtatcattat tatattaagt
     7321 tgaaccaatt taaataagta aagtaatgaa aacac
//
LOCUS       DAEMAE010000183         7351 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEMAE010000183 DAEMAE010000000
VERSION     DAEMAE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.185"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(255..680)
                     /locus_tag="KPH93_002963"
     CDS             complement(255..680)
                     /locus_tag="KPH93_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1689739.1"
                     /translation="MHGELKIKMYSFTMDCKDPYELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNPEYKPPVWPEKPEAQQQMAHMDFAVNDLEEAVQYAIRCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(838..1293)
                     /locus_tag="KPH93_002964"
     CDS             complement(838..1293)
                     /locus_tag="KPH93_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG1689740.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYELPIYQQSNNFQLYFEVDDIKQWESKINRIENIDFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALGEISKRTMYPVEFIIQFQ"
     gene            complement(1331..2164)
                     /locus_tag="KPH93_002965"
     CDS             complement(1331..2164)
                     /locus_tag="KPH93_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1689741.1"
                     /translation="MLKKESIRFINKNRSLLVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGSIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQKSGNISSQEISKKVFYSSRHLSRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(2438..3124)
                     /locus_tag="KPH93_002966"
     CDS             complement(2438..3124)
                     /locus_tag="KPH93_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689742.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILYIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLDKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(3139..5616)
                     /locus_tag="KPH93_002967"
     CDS             complement(3139..5616)
                     /locus_tag="KPH93_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1689743.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFISFSIGLSYE
                     SVQRKMAKGMSGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLIAADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRHTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYTVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTHILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPTVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSVLLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIISIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKNILPLYVMNNEISGNGVTLSRLEGTDNIELYNSM
                     FAINYTNFEIKKQVTEAFKDKRSVILNEDTLKKVGLSIGDTITLSKDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(5836..7248)
                     /locus_tag="KPH93_002968"
     CDS             complement(5836..7248)
                     /locus_tag="KPH93_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689744.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     TDIYDSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRLLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDYTLLKRAFSNLIVNAFTYGDKNTKVCVHITHI
                     TLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGG
                     NISVESILGLGSSFYIIFPI"
BASE COUNT         2577 a         1152 c          795 g         2827 t
ORIGIN      
        1 tattattcta aaaaatataa taattttatt tgttgtttta atattataac acatttgata
       61 atgtccaatt catatatgca aaatttatat actttaaaat atctaagaaa ttattcaata
      121 aaaaaatact atttttttaa attatcatta ttgataaagc tctactattt agatttttag
      181 tgtattcata ataagtaaaa gtacaacagt atatactgtt gtactttttt gtaattatta
      241 aacaatattt tggtctataa caatgaaaaa tggctacttt ccataattga tttcatctga
      301 cataagcaaa aaggatgtcc agctgggtca atcataactc tccagtcatc agtaaattgc
      361 tcctctgcga ttgtagctcc acaacgaatt gcatattgaa ctgcttcttc taaatcatta
      421 acagcgaaat ccatatgagc catttgctgt tgagcttcag gtttttctgg ccacacaggc
      481 ggtttatact caggatttcg ttgaaatgtt ataccaggat acgttccctg atttgttcct
      541 ggagcaccaa cacatgcata ttcttcatta taaaatgcta tttcccaatt gagtaacgct
      601 gcataaaatt ttgctaattc ataggggtct ttgcaatcca tcgtaaatga gtacattttg
      661 atttttagtt cgccatgcat aacgtaatta cctccaaata taaatttctt cgtcttttca
      721 tttttaagct aaattcaaac attaagtatg taatttattc caagtgtttt tctattatgt
      781 cacaagcctt tgtttttgca atataaagaa aaaattatat attttaatat tttataatta
      841 ttgaaattgt ataataaatt caactggata catcgtacgt tttgaaattt ctcctaaagc
      901 taagccttgt tttaaaagcc tttttatgac actttccata ctttctgaaa tttctataat
      961 atttttatca aaatcataaa acctaaaagt tctttgaccc catgggtatt cctttatata
     1021 atgtaaaaaa tcaatatttt ctattctatt tatcttactt tcccattgtt tgatatcatc
     1081 aacctcaaaa tacaactgaa agttatttga ttgttgatat attggtaact catatccaat
     1141 tatagcttta taattacttt gtaatccaaa tccattttca aatgatacat gttctcctaa
     1201 atctaatgta attttttgct ccataacatc ttcataaaac ttttttgatt tatttatgtc
     1261 tttgacaatt agcaatactc ctccaaattt catcttatca ctatcctttc tttgttataa
     1321 gtattataac ttaaaatttt gcaatctcac tgtaaaaatc agacatatta gctcggtatt
     1381 tttgaggagt tattccacag acaattttaa aatctttaat aaaatgtggt atatcataga
     1441 atcctaattc attacatata gacatcaatg aagttttatt attgtttaga agttttatag
     1501 atttattaat acgtaccaaa cgagaaaatg acttcatact caaccctagg tactgattaa
     1561 aaagtctgct taaatgccta gaactataaa atactttttt agaaatttct tgtgaagata
     1621 tgtttccaga tttttgaata atcaactgaa ttgccaaaat aaactcatat gagtattcaa
     1681 ttttaatatt agaaatcaat aattcttcca actttaaaat caatttatct atagataaag
     1741 attcattaaa tattttttta atttctaaat ctaatgaagt attaattata gaaaatggaa
     1801 caactttatc aataagttcg ctttgctgta tacctataag cgggaaaaaa ccagcaggtt
     1861 gaaattctat aattagtata acatcacatc tattagcaat acttcctact gtttttggtt
     1921 tagtagttgg accaaataat agactatgta atcctgtggc atcataaaaa aacaccaaag
     1981 ttacacttcc atgaggaata atcgtgtaat tatcagaaat tattgtttga tttggaaacg
     2041 atatagtaaa attcgatatc caatttctta attgtataca aggcaagaga taaacaaaat
     2101 tgtcttcaca aactagaagc gacctatttt tatttataaa acgaatactt tcttttttta
     2161 acatatatta tatcctgtcc tatcgtgaat atttattaaa ataagaagaa tccatactat
     2221 ctctacgtat cattaactag ttttaataat aaagatagaa tgaatactca ttagatattt
     2281 aataaacaaa taaaattaaa ataatcaata aaaaatatag tactcttatc tgttatttta
     2341 atattatagc acatttataa tatttaattt atatccactt tacatatgtc taatttatat
     2401 aattaaaaat gattatctca aatacaataa ttagtcctta tacaattaaa ccgtctgaaa
     2461 ctgtaatagt acgactacta cttttggctg cttctggtga gtgtgttacc ataattatag
     2521 tttgtccatt gtccctgttt attttatcca gtaaatctat tatttctatc cctgttttac
     2581 tatctaaatt tccagtaggc tcatcagcaa ataaaatctc tggttttcca attaaagctc
     2641 gagctatagc aacacgttgt tgctgaccac cagataactc tcgtggtgta tgtttacgtc
     2701 tatcagttaa accaactata tataagattt catctaattg atttttataa tcttttaaat
     2761 ttttaccatc taatagcaaa ggaagcatta tattttcctc cacatttaaa tttggtatta
     2821 agttataaaa ttgaaatacg aatcctatat ttctacgacg tattatactc attttctcat
     2881 cattaaaatg tgatatatca gctccattca tatacacttt tccactcgtt ggagtatcta
     2941 aaccacctaa aatatataaa agagtacttt taccagaacc agattgaccc atgatagaaa
     3001 caaattcacc tttcattact tgtaaagaca cctcttttaa cactcgagtt gttgtatttc
     3061 caattttata ttctttgata atattttttg cttctacagc taaactatta tgtgtaattt
     3121 tcatattaac ctccataatt attcaaactt aatttcttct atcagtttca ttttcttccc
     3181 ttttataata ggcactatcg aacctattaa agttactata attcccaata acccaacaat
     3241 aataaatgtt ttaaaatcta aatctggttt cattgaaatt tttggtcctg caactataaa
     3301 gatagtttga atctcaagta tagatattac aataccaata acagctccaa gtaaacctga
     3361 agtaaaccct tcaatcaatg tcacttttat attttgttta ttacttagtc ctatagattt
     3421 atacatagct atagaacgtc ttttttgaat ataattaatc agtagattat taataatacc
     3481 tactgtagca agaagaaaaa tgaaataggt catcttattc ataggagaca aaaaactact
     3541 tatagtattt aaagaatcat tattaaattc ttcaactgta cgactccaat tatatgtatc
     3601 tccaaataaa tcccttatct gaatcataac agcatctgga ttgactgctg tatatactaa
     3661 aaaaccataa ttagttttat caaaatcatt aactgcataa tgtgatggta taacagcttc
     3721 tacatcattt gcacgagatt taaaactacc tactatttta taagaaaaat catacttatc
     3781 tttagataga gttattgtat ctcctataga taaccctact ttttttagag tatcttcatt
     3841 caaaataaca gaacgtttat ccttaaaagc ttctgttact tgttttttta tctcaaaatt
     3901 tgtataattg attgcgaaca tagagttata tagttctata ttatctgtac cctctagtct
     3961 tgataatgtt acaccattac cagaaatctc attattcata acataaagag gtaatatatt
     4021 tttaattcca tccatatgtc taacatcttc aataaactct tcattcatct ttccatctgc
     4081 aaaaccttgt aattctgcgt cacgaaacac atctgttata taagttttga caaagttacc
     4141 aacaacgctt attgcaataa ctgcagaaat acttataaat agtaaagtga tattctgtat
     4201 tatatttttg ttatttttca tatttctagc agctaattta ccttcattcc ctaaaatatt
     4261 tttataaaca aactcaaaaa ctatagatat gatatcagta ataagaggaa tcaacacaat
     4321 agtagctaca attaatccta gtaatgaaaa tccgccagct agatacaatg tattttcagg
     4381 ggatattctt ggaagtaaaa cagataaaat aaacattata cttcctataa aaagtataga
     4441 acgatttgaa acattctttt cttctactgt tcctagtaca atatttttta ttggcaaatg
     4501 gctagctttt ttaacaggta tatatgcact aaatgaagaa acgattattg ctactataac
     4561 agatataatc actccaatgg gtgatattac tgttggaatt gatattcctt gttcgagaga
     4621 atttcctaac ccatgaagca ttaaatttaa aacaactaca ccaataggaa tagcaattaa
     4681 tcctcctata cttccgtata atatactttc aagcatcaat atatgagtta cagttttttc
     4741 attggcacca atacttctaa atgttccaat aaaaggaaga cgttctaatg taataacctt
     4801 ataactgctg tatatgataa atatactaag ggttaatgca aaaaaactta ttaaaaagaa
     4861 aggcattgtc ttttgtctag catctgaaat tatcttagtt tcatttatag tatttgatac
     4921 tgtatatttt tcagtagaaa gttcttcgga taatttattt actagatttt ctgtcatttc
     4981 ttcttctgac tctataagaa ctcttgtata tccactgcct ttatttataa tttttgatag
     5041 agtctcttta ggtagcagtg cattcacacc tcttgtatgc ctcaaaaata ctgtatcata
     5101 agatgcaata tcactaactt gaaaagtata agactttcca taaatctgta aagtaataga
     5161 atcacctttt tttattttat attttgatgt aaaacggttt ggaagaataa ttttatcacc
     5221 agaaaaatca gtaatactat cgccattttc taatcgtggc ttgttgattt tatttagttg
     5281 acttaagtct gctgctataa gactaaattc ttcatagtat ccccctttat tataaatagc
     5341 agagctttcc aatacaccaa cctttgattt tatagcattt aaatcaggta tgtcttctaa
     5401 attggttaat atatctggat ttttactttg gacagatata gtcgctgtac cagacatacc
     5461 ttttgccatt tttctttgta cactttcata agataatcca attgagaatg aaataaaaag
     5521 taaaactgtt gataaaacta tggacaataa cataactgct gttctaattt ttcgttcttt
     5581 aatatttgtg aaaatatact ttaatataat tttcaaattt acaccttctt cctttatcta
     5641 agatatttca gtataatatt tttatacttt aatccaaatt gaatctctaa tagactcctc
     5701 catactttta tcattctatt tataatattt ttaatcaaat cacctcctta aataaaaaag
     5761 attgataaac tactatataa atataataat ctatcaacct taatatattt tttatttgac
     5821 cttaattaac cttaattaaa taggaaagat gatataaaat gaactaccca atccaagtat
     5881 actttcaaca gatatattcc ctccatgaac ttcaataatt tgctttgtta ttgctagtcc
     5941 taaaccagtt ccttctattt tttgttttgt attgcttcct ctataatacc gattaaacaa
     6001 atttgatgcc tcttctcttg acatcccctt tccattatca gatataatga tttcaattct
     6061 ttcatttaat gttatatgtg ttatatgtac acaaacttta gtatttttat ctccatatgt
     6121 aaaagcattg acaataagat tactaaaagc tcgttttaat agtgtataat caaaatcaaa
     6181 gttaatatta tctatattac tcttaaattc aactatattt gattcatatt ctggataatt
     6241 taaaatatca atggataatt ctttcaagag tcttactaaa tcatagttat ttttatttaa
     6301 agggattatt ccatttttta attgataggt taattttaaa tcatttataa gtgtatttgt
     6361 atattctatg ttctttaaca tgattgaagc atatttatat actttttcag gtgaataatt
     6421 agtactacta tctgtaagaa gctctgcata cccttttatt ggtgaaagtg gagtttttaa
     6481 atcatgagtt atatttgaaa tccactcttc tctcataata tcagttttat tttgtatctc
     6541 atcacttcta tgaatttctt catttaaaat atttaaacta tcatatatat cagtaaaaac
     6601 acctttaata ttaactggaa gatagcttcg ttttgaaata tcactaacag cagatgtaat
     6661 atttgatatg cactttgtta cccagtaacc ataagcaatt ccagaaacca aaattattaa
     6721 aaatataatc cctatgattg aaaatacaat tgacttacca cctacaaatt tacttccatc
     6781 aaaaaacata ttcactttag atatatttaa tggaaaatat ataatataaa tatagtcatt
     6841 tttattattt ttaattgtat taaagtatac agttccatta cttgaattat tttggttagc
     6901 taatgttaat aattcagaat tagaataata atctttatgc tcttttggtt ctaaaaaccc
     6961 ataaactcta tttccactag aatctaaaat ttgcaaccac aattgatttt ctcgaagctt
     7021 ttctaatcca gactgtttta cttgtggctt attatctata aatacgattt gttcattaaa
     7081 gttttctgta aattttttcg gccagtcact acgaataaat tccccatttg gtttctgaat
     7141 agtaataagt gataagaaaa tacctattac tgtcaggatt aagcaaataa aaatcataaa
     7201 aaaaataatg tatgaatgaa atactgtttt ataactaggt ttatgcattt aatcaactct
     7261 tttcttaaac ttttatttta gtttgattgt tgcttatata aaatagacaa taattattga
     7321 catgtaaaaa ttcatttaaa aatacaactg t
//
LOCUS       DAEMAE010000184         7305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEMAE010000184 DAEMAE010000000
VERSION     DAEMAE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.186"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            665..1516
                     /locus_tag="KPH93_002969"
     CDS             665..1516
                     /locus_tag="KPH93_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG1689745.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTISIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1520..1990
                     /locus_tag="KPH93_002970"
     CDS             1520..1990
                     /locus_tag="KPH93_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689746.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            2003..3313
                     /locus_tag="KPH93_002971"
     CDS             2003..3313
                     /locus_tag="KPH93_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1689747.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGAEVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFNNAKDAKDILDAFTPVMTKEEYLTFLEKHDKTL"
     gene            3525..4271
                     /locus_tag="KPH93_002972"
                     /pseudo
     CDS             3525..4271
                     /locus_tag="KPH93_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4631..6172
                     /locus_tag="KPH93_002973"
     CDS             4631..6172
                     /locus_tag="KPH93_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG1689748.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSNVEKKGLKKAGISILIYVAI
                     IIALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            6207..>7305
                     /locus_tag="KPH93_002974"
     CDS             6207..>7305
                     /locus_tag="KPH93_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG1689749.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASYAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMI"
BASE COUNT         2559 a          983 c         1213 g         2550 t
ORIGIN      
        1 attatcatct taatttattt attactatat caattcactt attatcgtct tacttaattt
       61 acttatcgct atatcaattc acttattatc ctctcatttg ttatcatctt acttagttta
      121 cttattgcta taccaactaa cttattatta tctcacttat tatcatctta attcatttgc
      181 ttattatcat ttactttatt caatttctat atcaaaaatt agtatttgta cttaatactt
      241 aaaattaatt taaagtcttt ccatattatt tattttttat tgtaatatcc atttccatac
      301 tttctaagct ccttctactc atttatttta aaatttcaat ttactatttc tcaattctat
      361 attattattt ttcaatttta tattactaat tttaaagctt tttagctatt attcctacaa
      421 catttaaaca attttttcat aataaagcta tattacaata tattcaaaat atgtaatatt
      481 ttagatattt ttcgttgaca atgttaaatt tttgttttat tcttaattaa ggacatgtta
      541 ttgacatttc tgaaacttat ttgaaatagt tttttcattt tatttaggaa aacgtattat
      601 acttaagaaa acgtattttc tcatttcaga taagtaaaat atttattctt ttggggagga
      661 aattatggaa attaataaaa aacattttat tcaagtattt ttaacttgct taatcttggt
      721 agtaatatgt gaatttatag ggcaaagaac tatatctatt ggaaaagcct ctatagtgct
      781 gttccctatg ctttatgcag ttataattgg acttataata acaccagatt tattaggtaa
      841 aaaaattaaa gcacttaaaa aagtagttgg agaaaaagaa ataaatattg ctggtgaagt
      901 tgttggaatt gcactagtaa tgcttggtat aaaatatggt actacagttg gaccaaacat
      961 tgacaagata attcaagctg gaccagcatt tatcgctcaa gaatttggtc atatacttgc
     1021 accaatcgtt gcattacctc ttgcattaat gtttggaatg aaaagagaag ctgttggagc
     1081 tactgcaagt ataagtcgtg aaccttcttt aggagttata tcagaaaaat atgggataaa
     1141 ctcaccagaa ggtagtggtg tacttggtac atatttaatg ggaacagttc ttggtactat
     1201 atactttagt gtactaggtt caatatctat ttatagtggt cttcacccat atgctttggg
     1261 aatggcctgt ggtgttggta gtggaagtat gatgacagca gctgctggtt cattatctac
     1321 catggtacca cctgaaatgg cagatacaat acttgcctat gctgcaacaa gcaatatgat
     1381 gtctagtata acaggaatta tattcttagt ttttgtatcc ttaccattta ctaactttat
     1441 gtataagatt ttagaaccta aattttcgag aaataaaaag actaaagaaa ttaaatctac
     1501 agaaggagag tgctaataaa tgtttgctaa tttaaattta aagaaaagtt catatcagtt
     1561 attggttatg tctgttattg gagttataat tcttatagga caaagaatta gcgttggtac
     1621 atctatagtt acagctcttc ctggtatggt gatgctaata ttagctgcta tggcagctat
     1681 gattataaaa gatttatttc ctaaatcaat ctttccagct tttggatttg caactataat
     1741 cggtctttta cttagtatgc catatagtcc aacatctgaa gtattcttaa ctaacactaa
     1801 taatattaac tttatggcta taactactcc actactagct tttgcaggaa tttctgttgg
     1861 aaataaaata gaagctttaa aagaaatgtc atggaaaata gttataattt cacttattgt
     1921 atttacaact atattctttg catgtgcgag tatagctcat atagttttaa gtattcaagg
     1981 taaaatatag gaggaattta ttatgaataa agaacaatta aaatctctag taataaaaac
     2041 tatagatgaa aatagagaaa aaatattaaa aataggtcaa ggaatataca aaaatccaga
     2101 atatggatat aaagaattta aaactacaga agctgtttca aacttttttg agcaagaatt
     2161 aaatttatct gtagaaaaaa atatagcagt tacaggatgt aaagcagatg caaattctag
     2221 caagaaaggt cctcatatat ctatacttgg agaattggat ggtatatcat gtaaagaaca
     2281 taaagatgct aatgaaatag gtgcttctca tacttgtgga cataatattc aaatagctgg
     2341 aatgttaggt gctgctgttg gtttagtaaa aagtggcgtt ttagaacatt tagatggtaa
     2401 agtatctttt atggctactc ctgctgaaga gtttattgag ttggaataca gagaacaatt
     2461 aaaaaaagaa ggaaaaataa cttattttgg tggaaaacaa gaattaataa aaagaggtta
     2521 ttttgatgat atagacatat ctatgatgtt tcatgcttct gatttaggag aagataaggc
     2581 tctagttggt cctgagagta atggatttgt tggaaaaaag gttaaattta taggtaaaga
     2641 atctcatgct ggttctgctc cacatgatgg aataaatgct ttaaatgcag caatgcttgc
     2701 tataaacaac gtgaatgcac ttagagaaac ttttaaagaa tctgaaagag ttagattcca
     2761 ccctatcatc acaaaaggtg gagatatagt aaatgttgtt cctgctgatg ttcatatgga
     2821 atcatatgtt agagctagaa ctataaatgg tatgattgat gctaatgaaa gaataaataa
     2881 ggctttgatg gctggtggaa tggcagttgg agctgaagtt gagataactg aaattcctgg
     2941 atatcttcca atattacgat acaaaagtct tgataattta tttaaaaaca atcttgaaga
     3001 tttaggtcta aaaggaaaag ttattgatgg tggagatttt acaggctctt ttgattttgg
     3061 agatgtgtct catataatgc ctactcttca ccctatgatt ggtggtgtaa aaggagctct
     3121 tcatacaaga gattttgaaa tagttgatga agatttagct tacataattc ctgctaaagc
     3181 aatggcactt acagttgtag accttctttt taataatgct aaggatgcta aagatatatt
     3241 agatgccttt actccagtta tgactaaaga agaatatcta acattcttag aaaaacacga
     3301 taaaacttta taagtctatt tttataatag ggtggtatct actgtaaaag ttataccact
     3361 ctttgttttt gtaacagtat acttcttttc ttttagtttt taaattaaac caaaatatat
     3421 tatcctcctt aaaactttta gagctacgtt gaaatatcag tagaactatg atatattctt
     3481 tctaattaat ataaatttct attctaattc aaggtggtga aaaaatgttt aatgcaggaa
     3541 atcgaattat aaatacatgg ttgtacccta ttcaagatgg ctttgtcttg attgatactg
     3601 gatatgaaaa tggttttaaa catttcaaaa aacgaatttc taaattcaac ataaaaatca
     3661 aagatattcg atacatattt ctaactcatg ctcatgatga ccatgttggt tttctaaatc
     3721 agattctaga cgaatctcct aatgcaaaga ttgttatgca tgataaagct ttagaagttc
     3781 tatataaagg tcaaaactct tttattggtg gttgtaccag tcgtactgcc tttctctttt
     3841 gccaaattat gaaactattt ggaaaaggtg agcatcgttt ttctccactt aaacaagaat
     3901 ttgaaaaaag gtgtattctt gtgtctgaca agaatcgtaa agaaatttaa aaagaactaa
     3961 atggaaaaat tatagataca ccaggacata cttctgattc aatttcacta ctaattgatg
     4021 atggtgtttt attttgtgga gattctgcaa tgaatggatt tccaagtact cacaaaatta
     4081 ccatatttac agaaaacaaa ctagaattta ttcaatcttg gaaaacaatc atcaatacaa
     4141 aacctaaaat gatttatcct ggacatggaa aaccatttga atacagtaaa ttagaacaaa
     4201 atctaatcta tgtaaataaa ataaatttat atcctctaca tgattcaaaa gatattcaca
     4261 gatttttgta gaaaatacaa agtaaatttc tttgattaat taaaaaacct aacactttac
     4321 tacactactt attttaataa cttaatcttc cactagattt ttataaaatt ttgttttaaa
     4381 attaatttta aaggttatat actgcacaga tatattaaat acaattcgtc ataaatctac
     4441 ttaaagcata tttaactagc actactcaac cagaaaattc agaaaattgc attgacatag
     4501 gaaaaagttt attttatact ttttttagga catattgtgg acacgtctta aatcgacaag
     4561 tagaatttac attgcacgct taaccacaat acttttttta attatatttt taaacattag
     4621 ggaggatatt atgaatgata acgtagttaa gaagaaaaac ttttttgaca ggacattaaa
     4681 cagaatagaa actgttggta ataaactacc agacccagtt acaatatttt tagggttatg
     4741 tgtactttta cttatactat ctagtctagt tggttctatg ggtatatcag tagttcatcc
     4801 tggtactgga gaaactataa ctgctgtaaa tttattgact gtagaacagc ttcaaattct
     4861 tttaggaaac atagttagca atttccaagg gtttgctcct ttgggtctag ttctagtaac
     4921 tatgataggt gctggtgtat gtgacaaaac aggacttatg acagcaacaa tcaaagcatc
     4981 agtaagcaag attcctgaaa caagagtaac tcttgttgta atgactatag gtatgcttgc
     5041 caatatagct tcagatgcag gtacaatatt atttccacca ttagctgcac ttgtatatct
     5101 tggtgttggt agacatccac ttataggttt attttctgga tatgcagcag tctgtctagg
     5161 atttgccgca aacattatga taagtgtcaa tgatatactt gctgcaagtt ttacagttcc
     5221 agcagctcaa atgattgatt ctggatttca agccaatgca acaatgaact tattttttat
     5281 gatagcatca acttttgttc taatactttt ggcaacatgg gtaacagaaa aaattgtagc
     5341 tcctagattt ggtaaatatg aaggaaatgc agagttagat gttgatggaa gtcttagcaa
     5401 tgtagaaaag aaaggtctta aaaaagctgg tataagtata ctaatatatg tagctattat
     5461 aatagctctt agtgtaattg gtaaaaaacc attcttagct gacccagaaa caggagattt
     5521 actatcaaac aatgctcctc ttatgaaagg tatggtacct ataatagcac tagcattttt
     5581 tatacctggt gttgtatatg gaaaaactat aggcaaaata aagaaggata aggatgtagt
     5641 ctctataatg gctagtgcaa tgagtgatat gggcggatat ataatactag catttgctgc
     5701 ttctcaattt ttacaattat tcacaaatag taatttaggt ctgatactag ctgtaaaggg
     5761 cggagaattt ttaaaatcag caggaataaa tggaccttta ttgttgatag gatttattat
     5821 attatcgtgt tttataaatt tatttatagg tagtgcatct gcaaagtggg ctattcttgc
     5881 acctatattt gtgccaatgt ttatgatggt tggatacaat ccagcactta ctcagatggc
     5941 ttatcgtata ggtgattgta taacaaatcc acttagtcca ttatttccgt acttccctat
     6001 tatactagca ttcacaagaa aatacgataa agatgctggt atgggtactg taattgctaa
     6061 tatgatacca tactctatat cattcttaat agtgtggact atactattaa tattgtttat
     6121 ggtatttaat attccactag ggcctggaat acttccttca tatagcatgt aacttattta
     6181 aatctttcta aaaggagaaa taatttatgt tagataaaaa tagacttata aataatttta
     6241 tggatatggt tagaattgat agcccatcta atcaagaatt agaaatgtct aagtggttgg
     6301 taaactactt aaaagaaaga aatatagatg ccattataga tgatgcaggt gaaaaatatg
     6361 gtggtaatac aggtaatgtc attgcctata taaaaggtga agaaggaagt agaccacttt
     6421 gtttatgtgc acacatggac caagttcaac cttgtttagg tgtaaaacca atactagatg
     6481 gtaatgtagt tagaagtgat ggaactacta cacttggtgc agatgataaa gctggaatag
     6541 ctgctatact tgaagcttta gaacatgtta ttacagaaaa aataccacat agagatattt
     6601 atctttgttt tacaatctgt gaggaagctg gaatgcatgg tgtcaaaaac tttaatcctg
     6661 acaacttacc ttgtaaagat atggtgattt tagattctgg tggagctatt ggttcgatag
     6721 catacaaagc tcctgcccaa caatctataa aaatatcttt tcatggaaag aaagcacatg
     6781 ctggtataga acctgaaaag gggttaaatg ctatattagt ggcctcttat gcaataagca
     6841 atatgcacat aggcagaata gattcattga ctacttcaaa tataggtaaa atagaaggtg
     6901 gtggagctac aaacatagta actgataagg ttacattgac tgctgaaatt aggtctcata
     6961 tacctgaaac tttagagtat gagcttaatc atatggaaaa atgttgtaag gatgctgcat
     7021 ctaaatttaa tactacttat acttttgaac ataatatgtc ttaccctagt tttgaactta
     7081 gtagagatag tcacgttttc aagttaagtg aagaagcaat aagacaagtt ggtattgttc
     7141 caaatccaat ggtaattggt ggtggaagtg acgcaaatat attagctaat cttggatata
     7201 attgtgctat attaagtcta ggtatgtatg atgtacacac agttaatgaa tatgtaaata
     7261 tagacgaatt atatgatact gccaaaatag tttatcatat gatta
//
LOCUS       DAEMAE010000185         7264 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEMAE010000185 DAEMAE010000000
VERSION     DAEMAE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.187"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            222..1391
                     /locus_tag="KPH93_002975"
     CDS             222..1391
                     /locus_tag="KPH93_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689750.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            1772..6025
                     /locus_tag="KPH93_002976"
     CDS             1772..6025
                     /locus_tag="KPH93_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG1689751.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYKKLGNINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDANIIFPENAQLYIAIGAGLLSVDEDSIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEIYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEINHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRLRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAMYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKTSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            6174..6668
                     /locus_tag="KPH93_002977"
     CDS             6174..6668
                     /locus_tag="KPH93_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG1689752.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNEYIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            6784..7161
                     /locus_tag="KPH93_002978"
     CDS             6784..7161
                     /locus_tag="KPH93_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG1689753.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         2923 a          752 c         1326 g         2263 t
ORIGIN      
        1 tatatattta gtaaggaatc atacttttta taaaatgaaa gataagatgt cgaaaacttg
       61 cataaatctg aaaaattttt attattaaaa tactgatttt cattttaaaa atgataaaaa
      121 aatgacaaat aaagttgcaa atagtaatac ttataagtat aattaaaaaa gagaaaaggt
      181 tttgttaaac tttataacaa aaaaagaagg gatgattcaa aatggagaag ttaacagaca
      241 ggagacaggt taaggcagta agcatggaaa ccttcatttt cctaatatta ctagtagttg
      301 gttttgggta tgttggtagt attatgggag caggaatgat gttcaaagta ataatgagta
      361 cagcacatgc tttactatta gagacagttt tcttaattat ggctatggca gttcttgcag
      421 gcgcattaag cgcactactt tcagagtttg gagtaatagc attaattaat aaaattttcg
      481 ctgtatttat gaagccttta tatggactac ctggtgctag tatagcagga gctataacaa
      541 cttatttatc agataatcca gctattatac cttttgctaa agataaaaca ttcacacagt
      601 attttaaaca atatcaagtt ccagcattat gtaacttagg aacagccttt ggtatgggac
      661 ttatattaac aacatttatg atttctcaag gtacagaata tgtacttcca gcattaattg
      721 gtaatttagg tgcgattata ggtagtgtta taagtgttag aataatgttg acatttacta
      781 agaagtttta taaatacaat ccggaagaag ataaagcaac aggaacatta gagaaaaagg
      841 atgaatttag agaaatcaga gaaggaaatg tatttcaaag agctttagat gcaattcttg
      901 aaggtggtaa aatgggggta gatatgggaa tggctataat tcctggagtc ctagtagtat
      961 gtacattggt gatgttatta acttttggac catctacaga ccctgttaca ggtcaagaag
     1021 tttatacagg agctgcatat gaaggaataa aattacttcc agttattgga gataaacttg
     1081 gatttatatt agaaccatta tttggattta catcaccaga ggcaatagca ttccctataa
     1141 cttcattggg agctgttggt gcagcaatgt ctctagttcc agagtttata aagtcaggag
     1201 ctataactcc taatgatata gcagtattta cagctatggg tatgtgttgg agtggatatt
     1261 taagtacaca tattggtatg atggatgcat taaatgctag acagttggca ggtaaagcga
     1321 ttctttctca tacaataggt ggtttatgtg caggagcagc agctcacttt atatttacac
     1381 tcgttggata aacataatca gctaagataa aaaataatat acaaaaaaat ggttgttgat
     1441 atgtaataag aatttacata tcaacaacca tttttttctg actaaagata atttataata
     1501 cataaacatt aagtggatat tagagttaag ttatatttat gtaaaaagta acaaaaatct
     1561 gtagaaattt atgacaaaat ttagtacact aactatatgt tcttatttaa aatttaaggt
     1621 tagaattagg aattacctag tagagtaagc tttgattaat aaaagaatca taaaataaaa
     1681 atattagtca taaagattat aaataactta cttaaaataa agccatagaa cttacaaaaa
     1741 ttaaaaaagc atataaatat ggaggggtac aatggatgaa ttatttcata taggaattga
     1801 tgtaggttct acaacggtga aggttgtggt acttaataat agtaataata tagttcataa
     1861 agaatataga agacattatt ctgatgtaaa aaaatcagtt aaagaagtac taaatggtat
     1921 atataaaaaa ttgggaaaca taaatactac cattataata actggttcag gtggaattgg
     1981 aatatcaaaa aaattaggcg taaaatttgt tcaggaagtt atatctagta caaaatcaat
     2041 agagtatttt catcctgaaa ctgatgttgt catagaactt ggaggtgaag atgcaaagat
     2101 aacatatcta agtggaggaa tagaccaaag aatgaatgga atatgtgcag gtggcactgg
     2161 agcattcata gaccaaatgg cgtcactttt aaagacagat gccagtggat taaatgaact
     2221 tgctaaaggg tataatgtta tatatcccat agcttcaaga tgtggtgttt ttgctaaaac
     2281 agatatccaa cctcttataa atgatggagc taatcagact gatatagcaa tgagtatttt
     2341 caatgcagtt gtcgtacaaa ctgtgagtgt tctttcttgt ggaagaaaaa tagaaggaaa
     2401 tgttgcattt ttaggtggtc cattgtactt tttatctgaa cttagagaag catttaaaag
     2461 agtattggat ttaaaagatg caaacataat tttcccagaa aatgcacaac tttatattgc
     2521 tattggggca ggattattat cagtagatga agatagtata caactaaaat ctctaataga
     2581 aaaattggat agtataaaaa atattgaaga tggagaagta aatttattag aacctttatt
     2641 taaagataaa aatgagtatg aagaattttc aagaagacat gaaaaagaaa aaataaatta
     2701 tgtggacata aattcaataa aaagtaattg ttatttagga attgacgcag gctcaactac
     2761 aactaaagca gcacttattg atgaggatgg aagacttgta tattcttatt ataatagcaa
     2821 tgaaggaaat ccattaaaaa caaccattaa agtcataaat gaaatatatg atatattacc
     2881 aaaaaatatt aagatattga gttcaactgt aactggatat ggagaaggtc ttattaaaaa
     2941 agctcttaaa atcgataatg gagagataga aactatagcg cattacaaag ctgctaaatt
     3001 tttcaataaa gatgtagatt ttatacttga tattggtggt caagatatga agtgcctaaa
     3061 aataaaagat ggagtaattg ataatataat attaaatgaa gcttgttcat caggttgtgg
     3121 ctcattttta gagacatttg caagttcttt atctatgaca atagaagaat ttgcacatga
     3181 aggtatttat tctcaaaatc ctgtagattt aggttcaaga tgtactgtat ttatgaattc
     3241 tagagtaaaa caagctcaaa aagaaggcgc aagtgttgga gatatatcgg caggactatc
     3301 ttattcagta ataaaaaatg ctttatttaa agtaataaag attagagatt taaatgagat
     3361 tggtaataat attgttgtac aaggtggaac cttttataat gatttagtgc ttagaagctt
     3421 tgaaaaacta attggaaaaa atgtaattag acctaatatt tcaggaatta tgggagcttt
     3481 tggttcagca cttatagcta aagaaaaata tgaaagtgga tacaaaacgt ccttgttatc
     3541 taaggaagag ttgaataata tcaagctaga agcaagtgta actagatgta aagggtgttc
     3601 aaatcattgt cttcttacaa taaataaatt ttccgataat gagattttta tttcaggtaa
     3661 tagatgtgag aaaggtgaag ctatttatgg cgataaaaaa ataggattag aaaagaaaaa
     3721 accaataaat ttatttaagt acaaatacaa tagaattttt agatacaaac cattggaaaa
     3781 agaagaagca aaaaatgggg aaataggtat accaagagta ttaaatatgt atgaagatta
     3841 tccattttgg tttacttttt tcaatgctat gggctttaga gttgtacttt cagataggtc
     3901 atcaaaacag ttatatgaaa ctggtataac tagtattgca tctgaaacag tttgttatcc
     3961 aggaaaatta gttcatgggc atatagaaaa tttaattgca aaaggtataa aaaatatatt
     4021 ttacccaagt gtaacaaatg aaaataaaga ggattcaaat gcagataatt attataattg
     4081 tcctgtcgta atatcatatt ctgaagtaat aaaaaacaat gtagagaata taagaaataa
     4141 gaacataaat tatataaatc catttataag tctaaatgac aaggaaaaat tgaagaaaag
     4201 actatatgat gaattatcaa gacatttttc tgatataaaa ataactaaag aagaaattaa
     4261 tcatgcagtt gataaagcta ctgatgaaca gaatttattt aaaatggaga tacaagcagc
     4321 aggtgaaaga gcacttaaag aaataaaaga aagaaatatg aagggaattg tgttatgtgg
     4381 aagaccatat catatagacc cagaaataaa tcatggaatg ccagagctta taaattcatt
     4441 agatatggca gtgcttactg aagattctat atgtcattta gccaatgtac aaagaccact
     4501 tagggttgta gaccaatggg tatatcattc aagattgtat aaagcagcta gttttgtaag
     4561 agataaaaac tatttggaac ttgttcaact taattcattt ggctgtgggt tagatgcagt
     4621 aactacagac caggttcaag aaatacttaa tgaaaaatcg aagatatata ctattataaa
     4681 gattgacgaa ggcaacaatt taggagcagc taaaataagg ttgaggtctt taaaagcagc
     4741 tatgtttgag agagaaagca aaaatataga tgtaaaaaat ataaaggtaa acaggataca
     4801 gtatgctaaa aataatcaga taacgaacaa acatactata ttagcaccac aaatgtctcc
     4861 aatacatttt caattccttg aaaaagctgt taacttaagt ggttataata tagaaattct
     4921 aaaagatact gatagtagtg taattgaaga agggttaaga tatgtaaata atgatgcatg
     4981 ttatccagct ataatagtag taggtcagct aataacagca ttaaaatctg gcaaatatga
     5041 tttaaataat acttctgtta ctataactca gactggtgga ggttgtagag ccacaaatta
     5101 tattggattc ttaagaaagg ctatgtatga tgctggattt aaagatgttc ctgtcattgc
     5161 acttagtgta aatggaatag aagatagtgg tataatggat aatatatcac taaaattaat
     5221 caatagactc tttatgtctg ttgtttatgg tgatttgctg atgaaagtac tatatagagt
     5281 aagaccatat gaaaaagtta ctggaagtgc aaatgctcta tatgaaaaat gggttgatat
     5341 atgcaaaact tctcttgtta aagcaaaaat ttctgtattt actagaaata taaaagatat
     5401 agtaaaagaa tttgataatt tagagatact agatatcaaa aaaccaaaag taggtttggt
     5461 tggagaaata ttggtgaaat tccatccaat tgcaaataat aatttagtag atatattaga
     5521 gcgagaaggt gctgaagcag tcgtaccaga ccttacaaat ttcttcttaa gttgtgcctt
     5581 taatactata tacaaacaca ctcatttaga aggaagtaga aagagtagaa tgataggaga
     5641 agcatttata tacataactg gaatatatca aagagtatat aaaaaagcac ttgataagag
     5701 tgagagattt tatgctccag ccaatataaa aaatgttgct aaaagtacag aaccagtagt
     5761 gtcattaggt aatcaaaccg gagaaggttg gcttttaaca ggtgagatgg ttgaattatt
     5821 aaatgaaggt gtagaaaaca ttatttgtat gcaacctttt ggatgtttac ctaatcacat
     5881 aattggtaag ggttctataa aagaattaaa gaggttgtat aaaaatgcaa atataatacc
     5941 tattgattat gacccttctg caagtgaggt aaatcaactt aacaggataa aacttatgct
     6001 ttctaaggct tttaaaaata tataagattt atattttcag atgttaaaag ttaatgaatt
     6061 taaaaatata ctctatatta agtatctata aaaaagagtt atcctatttt ttaggataac
     6121 tctttattag ttaaaaaatt atatgtatac tgatattaaa ggggggaagt aatatgagtt
     6181 tagtatatga tgtttataaa tcaaaattag gaaatttgta catattaagt gaagatggtt
     6241 ttatagttag tgtatacata ggagatgaaa aatttaataa tttaaaaaat gaatatatta
     6301 aaaaaggaat tgatataaag agaagtaagg aagatttaaa ggaagcaata agtcagatag
     6361 atgattactt taatggaaaa agaaaagtct ttaatttaaa tattcgtatt gaagggactg
     6421 aatttcaaaa atcagtttat agggaaatgt taaaaatacc ttatggagaa acattgtgtt
     6481 attcagatat agcaagaaat ataaacaatc caaaatcagt aagagcaata ggacaagcaa
     6541 gtaaaagtaa taaaattcca ataataatac cttgtcatag agtagttggc aaaaaagata
     6601 ttggtggata tatgggaaat cattctgatt tgaaagagat actattaaat ttagaaagac
     6661 agagttaact tagaaagaaa tgtttttatt gttaatattt gatataataa tagtaaaaag
     6721 aagtattttt tacaaaaaca ataaagaatt tattttaatt tacaaaaatg aggaggaatt
     6781 ttaatgtgtg ataaagttat agcatcaaaa tatcatgaga agggatttaa ttgtgcagaa
     6841 tcagttataa aagcatataa tgaggaattt aatacagata taccagtttg tttaggaagt
     6901 ggactgggtt cagggtgtgg ggttgctagt ttatgtggtg cagtaaatgc atcaaatata
     6961 ataattggat atgtaaaagg cagaaaccac gaagatgaaa gtactaaagc taaagtatat
     7021 gctaaagatt taactacaac agtaagaaaa gagtatggtt cagagttatg tatagaccta
     7081 aaaaaagatt tagttgcttg tagagaaata atggatttcg catatgatag tctaaaggaa
     7141 actttaaaaa aagaattata agaaaataag ctatcaaata tgaattattt gatagcttat
     7201 ttttatagtc gatattaaat aaatcaaata tattttagaa ttattatttg taaataaaca
     7261 gcaa
//
LOCUS       DAEMAE010000186         7238 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEMAE010000186 DAEMAE010000000
VERSION     DAEMAE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.188"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..758)
                     /locus_tag="KPH93_002979"
     CDS             complement(<1..758)
                     /locus_tag="KPH93_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG1689754.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYVWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAVYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPVWIILGIVFYLCRGKKFRLSLYYVLMCIFIFVAMANGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLF"
     gene            complement(769..2172)
                     /locus_tag="KPH93_002980"
     CDS             complement(769..2172)
                     /locus_tag="KPH93_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689755.1"
                     /translation="MKWKITRNFIFTIVFVVISVVIINIISILYVVSTDSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKNYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVHVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDSGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2176..2901)
                     /locus_tag="KPH93_002981"
     CDS             complement(2176..2901)
                     /locus_tag="KPH93_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689756.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLSNIYTAKTKEEGLEVFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDIQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(3013..3786)
                     /locus_tag="KPH93_002982"
     CDS             complement(3013..3786)
                     /locus_tag="KPH93_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689757.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLLFFGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLLANGLTL
                     SIINISFILLLGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIIAIPYFSVGFIPDKAIKFMSLTPNWIMMSQQ
                     IVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(3792..4679)
                     /locus_tag="KPH93_002983"
     CDS             complement(3792..4679)
                     /locus_tag="KPH93_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689758.1"
                     /translation="MNKLEIEKLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDDDYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKHLNIKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPCGANIQPVQ
                     AKFEDVYMFYFDLDDAREV"
     gene            complement(4693..5715)
                     /locus_tag="KPH93_002984"
     CDS             complement(4693..5715)
                     /locus_tag="KPH93_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689759.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILIISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTDEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRVVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAVFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VISNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAISLCFLVIK
                     SDYISR"
     gene            6283..6459
                     /locus_tag="KPH93_002985"
     CDS             6283..6459
                     /locus_tag="KPH93_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689760.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2531 a         1135 c          655 g         2917 t
ORIGIN      
        1 taaaataaat acttcatact cttccctttt tctccattgt aaagtaacat taaaggtaga
       61 gaagcaatca tcatccattg gtaattttgt aaaatagaat tttttaaact atagtctcca
      121 tttgccattg ctacaaatat aaatatacac attaaaacat aataaaggct taatctaaac
      181 tttttacctc tacacaaata aaaaacaatc ccaagtataa tccaaactgg tccaccttct
      241 actaaaaatg gtactggtac caaaatcata aataaagcaa taactctgtt agtgacttta
      301 aaaagtaaag ctactgtaaa tattcctaaa attataggaa tgattatcat caataatcct
      361 ataataaaat atttaatttc tttttctttt ctaaatttct ttataaactc tattgcttgt
      421 aaataaaccg ctattaaaaa taatgttgaa aaaatgttat ttataattat agctccttct
      481 ggatgtgaaa atacattatt aattatttga tttcctatag ccataataac agaccaaaca
      541 tacaatctac ttatatattt ttttctgtta ctggtgtgat aaaatccttc aacagccata
      601 aatataaata gtggtgctga tattctgcct acccaatgaa accaaattgg tatttgcatt
      661 gtttgtggca taaatgctgc tatatggtca aatgtcataa atattaatgc taaaatctta
      721 atagtaaacc cacttatacc ttttttaaaa aaatccatat catatctcct aattttgttt
      781 tatctctata ataatttttg ttcctattcc aatttcacta cttacataaa ttttaccttt
      841 atgaatctct attatttctt tagaaatagc cataccaaga cctgaacctt tatgcatctc
      901 ccctgtatta gtccctctat aatatttttt aaaaatatgt ttcaaatcac ttttgcttat
      961 tccaattcca ctgtctttta ttataatttc tatattatct ttttttacaa tttctacact
     1021 tatcaaagtt ccttcactat tatgaactat tgaattaaat ataagattgg ttatagccct
     1081 tctgaataag attgaatcta catgtacttc aattaaatct tcattgcttt caaactcaat
     1141 attcctattt ttatatctat tatcatttaa aatatcaatt attatatctc tgactaaact
     1201 aactaaattt atttttttct tgtccagaac tatagtatta ttttttaatc gagtagataa
     1261 atttaaatca tcaactaaat cttttatata cttagactta ttatatataa tttctgtata
     1321 ctctcgtatt tcatcttctt caaactcata atttttgtca ttaattatct ctgcataccc
     1381 ttgtatagat gctagaggtg tctttatatc atgtgttata tttgccagcc attcctctct
     1441 caactcttca ttttcttttc tctcaacttc atttactctt aaagtatcag ctaacatatt
     1501 tatatttttg aaaacttctt catatatacc tctgtcctta agatataaac tataatttcc
     1561 attagccata gtctcaatac tccatagtac gcttgaaatt ggttttgtaa gttttctact
     1621 aaataaatat cctacaccta aagctaccac gctatcaatt actagtgtta taatgataac
     1681 tttatttaaa gtttttttaa tgttattttg agaataggtt aaaattattc ttgatatatc
     1741 tctactaggt atacctacta ttatgtttag atgttttttg tttaaatatt tttctagtac
     1801 aaaattaaca tactttgttt ctatatactt gtaattattc accatttgaa agggtgtata
     1861 tttttttgga gtatcctttg gcacatttac tccataaact tcctctccta aatcatttaa
     1921 aacctgtatc catgaatttg atttttctaa tttttcagct ccaatcttag atagtttaaa
     1981 ttctccatct ttctcatata aatctttttc aaaagagcgt gcaaattctt caggattatt
     2041 tccagaatct acaactttaa aaaaactatc agttgaaacc acatacaata tagatataat
     2101 atttataatt actactgata taactacaaa cacaatcgta aatataaaat ttctagtaat
     2161 cttccacttc atattctaat cttcctttac tgctaattta tatccaagac cttttattgt
     2221 cagaatatat tcaggctttg aaggattatc ctctaatttt tccctaattt ttcttatatg
     2281 aaccattata gtattgtcaa atccaacata atcttctccc cacactttat cccatagttt
     2341 ttctttactt ataatttgat tttgattatg tgcaaataaa gataggattt ttagttcctt
     2401 tgcagacagt cctatagatt ttccatcttt tataagttct gccctacttt catttaatat
     2461 atatgggcca aatgatataa ttttttcttc attattattt aaatttttac tttcattttg
     2521 aatatcacta aaataactta atcttctcaa atgagcttta actctaaaag ccacttcctt
     2581 agggctaaaa ggttttgtaa cataatcatc tcctccaatc gctagtccaa gtaacttatc
     2641 taattcttcg gttttagctg ataaaaatat tattggagaa ttggaggttt ttcttatttc
     2701 tttacagata tcataacctt cgccatctgg taacataata tccaacacaa tcaaatcagg
     2761 attaatactt ttaaatactt ctaatccttc ttctttagtt tttgcagtat atatattgct
     2821 aagaccctct ttttttagga cagtttctaa aagcttcaat atttctacct catcatctat
     2881 tattaaaaca cgattttcca taaataacct cccgtaataa tttacatata tattagcata
     2941 tattttatct aaaattcatt tatcttattt ttaattaaac taaaatataa ataaaattac
     3001 tattattttt agttaaaacc ttttgcttga tgtaaaattt tcatatgtta ttttagttaa
     3061 cactatcatt aatataattg agattactat agaaaaaact atcaaaatac ttggtacatt
     3121 atatctaaca atttgttggc tcatcataat ccaatttgga gtcaatgaca taaattttat
     3181 agctttatct ggtataaaac ctacactaaa gtatggaatt gctattatca atccacctag
     3241 tataaatgga attacagcag atttaaatat agatgaaagt gttaagccta tttgtgcaaa
     3301 tataaaagag gcaaaaatag tagtcaatat aacaattata tacatttgta ttactgtaag
     3361 atttaaatta ctatttccaa agtaccataa gttatgaatc ggttcgttta aatttttaaa
     3421 atcaaaaaaa ctaaaacctc taattcctaa taacaatata aaagaaatat taataataga
     3481 taaagttaat ccattagcta atagttttga taatactact gatttttttc cctttttagt
     3541 tgtatttatt aaacctgtca tattagaatc atattcttta atgtaatttc cagaagttat
     3601 tataaacatt atcaaaccta ttagaggtat tgagccttct atcttgtaaa atatatctgc
     3661 ataagtatct aaaacatcaa actttctaat cagaagaaat ccaaaaaata gtaaaaacag
     3721 ccaaattatc caaacacttt ttgattttaa tatttttatc atttcagctt taactaaata
     3781 catcatttct actacacctc ccttgcatca tccaaatcaa aataaaacat gtatacatct
     3841 tcgaattttg cttgaactgg ttgtatatta gccccacatg gaatatcctc tgaaacaatt
     3901 ctaagtacta ttgaatcaat gtcactttga atacttacaa ccttatattt agattgtata
     3961 tcatcaactt cacattcatt tttaacttga acattatata ctttattttc catgtgactt
     4021 aatatgtctt tgtgtgttcc tttcattagg agtttgccat ctttaatcat tattgtttcc
     4081 tttgcaactg attctatatc ggaaattata tgagtagaaa gtattattat cttatctcta
     4141 gacatttttg ctattaaatt cctaaatcta gctctttctt gtgggtcaag acctgctgtt
     4201 ggttcatcaa ggactattat cttagggtca tttagtaaaa cttgtgctat cccaactctc
     4261 ctcttcatac ctcccgaaaa ttttcctata gacttatttc tcacatcata aagacctact
     4321 aaatctaata attctcctat cttaatattt aaatgttttc cccttaaacc ttttaaagct
     4381 ccaatatact gtaaaaattc ttttgctgta aaatttttat aaactccaaa ttcttgtggc
     4441 aaatatccta aaatatttct ataatcatca tccattttaa atatatcctc accattataa
     4501 agtatctgac cttcatcagg ttttatcaat gttgttattt gtttcattaa tgtagatttt
     4561 cctgctccat ttggtcctag aagaccataa actccatttt ccagagttac tgtaatcttg
     4621 tcgtttgcta cttttttacc atatatcttt gaaagttttt ctatttctaa tttattcatc
     4681 ttaactcctc ttttatctac ttatataatc tgattttatt actaaaaaac ataatgatat
     4741 agcacctatc aatattgcca tcaaaaggcc tattgctata ttgacaatat ctatattcac
     4801 tttaaatatc ataatatcac ctagataatt tcctattatt ttattaaaac caaatatcaa
     4861 atcataataa tttacatttg aaataactga aagtaatttt ttaggtaata tatggattac
     4921 tgacattatc ttagttatag atataataaa tataaaaaaa cttattgaag atattgaaga
     4981 tttacttata aaagacccaa atacggctat catggcccat ccttcaaaca tcaaaagctc
     5041 tatgcctata tttaatatta catactttgt tatacttaaa ttagttttca atgcaactac
     5101 attgtctact actctaaaag attctaagtc tccattaaca ggtactcctt gttgcataca
     5161 agttataata aatattccca ttaaataaac agaataaatt actaatggaa ctaataaagc
     5221 aacaaatatt ttagatttta atgctttttt cttctctttt gatgatagta taatatcttt
     5281 tactgaagta acgacttcat ctacgtaaat attggatata attatagata taatgattaa
     5341 cattcctaca tttataagtg aatttgtcac tctatagtta aatactttcc aaaaactaat
     5401 atccttatat tcattcacac ttaaattatc aagttttttc tttgacatta attcaatttt
     5461 tttagaaaat tcatctgtat ctgtttgatt tgaaaactgc tttaaaacat taatcttatt
     5521 ttgaaatttc tcattaccta tgtcgatacc acttctcgta tcttctatac gtcctttttc
     5581 tttatctata taataacttt ctgtttctaa aacaggtgta ataaagatac taattattag
     5641 tattaaaact aatatcccta gagatataaa catactctta gatttagcca atttttttat
     5701 ttcccaattt accaatatat ttctcctcct aaatcatttt aattacacat atatattaaa
     5761 acttaaatct tatataaata taatcttaac cttaaataaa gcttaaatta ttttataatt
     5821 tacaggtgtg tttattattg gagttacatt tattattttt ctgggaaatt atacattttc
     5881 aattattttt gatattttta ttgattggaa attttattgt tgttcaaatt gtagttattc
     5941 tttaaattta aagatggagt tttttaagaa tagaacacct actgtccttg ttgtggaaag
     6001 attattaaat agttgcatat aactttattt atcttgaatc ttctttttaa aaacaattat
     6061 aattttagaa tattttctat tctcaaacaa gcatgttgaa taatgaagca tcttctagta
     6121 ttatatactt gtagaataaa actaaattgg taaaactggg aatatttaac cttaaggagt
     6181 taagttatag aaactagtac taaaaaattt tataaattat gttaattgac taatagaatg
     6241 ggacttttta tttttactaa attttttagg gggatggata atatgtttat tggagaaata
     6301 gacaaatgta cacatatact aactgcttac atcagtagtt catatgatta ttgtaatttt
     6361 ttagatacac agttagatga ttttatatca gaatatggag aaacggtagt tgaaacctgc
     6421 ttacatcaag tcttattatt agtaagtaga tacaattaaa attaaatttt tctagaaaaa
     6481 cctttttaga tttatctact ctaataaata tataattttt tgtgctttat aagtaactat
     6541 aacttctgct aaagtaaaga tgtattggga gtacatcttt actttcccat tatagaagtt
     6601 aaaagcatac acgataaaat caattataca atacaaatta tatacttctt atatctaact
     6661 taatttacaa gaatcatcct atatgtatat tctgcaatcc actaaacatg gctctaaatc
     6721 tttactattt tatacattct ctataaatca tacaaaccat atataaaatc cccctaatat
     6781 gtttaaacca tatataaaat aacaccaata tttagaatat tcaatatcaa ctgcataact
     6841 ggtcaataaa aacgtataac tagacaaatt tcaatattta tataaataat acaagaaaaa
     6901 caagcatttt atttatataa tttttttatt ttttgtttat attaaaaaac tatattgatt
     6961 aatttatttt atttagtaat tataaaaatt cgtcttagaa tatattctat atttgtagat
     7021 tagctttaat atttttaatt taacaaaaat taagctccca atatttttat ccacttttat
     7081 ctcattaatt aattatatta tttttatgct ttttacaatt tttcctattt aaatatatat
     7141 taatctaatc ctctttcata aaataaatgt tactcttatt aattaaaaaa attataacca
     7201 cttttgattg ctaatcaaaa gaaaatatct aagtaaaa
//
LOCUS       DAEMAE010000187         7189 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEMAE010000187 DAEMAE010000000
VERSION     DAEMAE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.189"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(507..1334)
                     /locus_tag="KPH93_002986"
     CDS             complement(507..1334)
                     /locus_tag="KPH93_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG1689761.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            1716..2036
                     /gene="sugE"
                     /locus_tag="KPH93_002987"
     CDS             1716..2036
                     /gene="sugE"
                     /locus_tag="KPH93_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG1689762.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(2169..2849)
                     /locus_tag="KPH93_002988"
     CDS             complement(2169..2849)
                     /locus_tag="KPH93_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG1689763.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            3245..4297
                     /locus_tag="KPH93_002989"
     CDS             3245..4297
                     /locus_tag="KPH93_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1689764.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVKSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLTKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4344..4760
                     /locus_tag="KPH93_002990"
     CDS             4344..4760
                     /locus_tag="KPH93_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1689765.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNYGDSI
                     DEFSRRLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            4789..5262
                     /locus_tag="KPH93_002991"
     CDS             4789..5262
                     /locus_tag="KPH93_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1689766.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            5309..6067
                     /locus_tag="KPH93_002992"
     CDS             5309..6067
                     /locus_tag="KPH93_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1689767.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            6091..6930
                     /locus_tag="KPH93_002993"
     CDS             6091..6930
                     /locus_tag="KPH93_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1689768.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLSFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
BASE COUNT         2603 a          836 c         1299 g         2451 t
ORIGIN      
        1 cagttgctta aaatatactt gatatatcaa ttattattgt tttttcagtt gcttaaaata
       61 tacttgatat atcaactaat gttgtttttt aattaaaaga aattttagga aagataatat
      121 caactaggag agataatatc aacattgaag aaggtagagc aagatttcta cataattgtg
      181 aacattatct ggcatgtgtg cattggtgtt ttaagcaagt aattcaatag aaagatgtga
      241 ctaaaaatag aaagaaatac cataatccaa atgtaaagct agaagacata ataagtaaaa
      301 aagtaaagat atttaataaa agaaataact aatagtgatt aaattagtaa atgctgataa
      361 ttagcataat cattagtaat tattagtatt atttaattaa attaatttta gcattatttt
      421 tatgaaatta attttaaatc aaattaatga gaaccccata aattttttta acacttaatt
      481 tatgatttat ggggttatgt tctgttttat ttcaaagtat taagttcata taaatcagtt
      541 cttctgtgct taagaagtgg taattgttgt cttatgtcag gaattaaatc tctatcaatg
      601 tcagcataaa taatacattc tttttcatca gcttgagcaa ttattcttcc ccaagggtct
      661 gaaattcttg aatttccaaa agctatataa gaagcattca tatttctagc aggtgcagca
      721 ccaacataga atatctgatt atctaaagca cgcattctta tagataactc ccaatgggcg
      781 ggaccagtag tcatattaaa tgcagcaggt aagataacga tttcagcacc tttaagagcc
      841 attaaacgtg ataactcagg gaaacgaata tcatagcaaa tagctatacc aacttttcca
      901 tattcggtat ctattacagt gacatcattt ccagctgtaa gagtatctga ttctttaaaa
      961 ctaacttttc ctgtaacttc aatgtcaaat aagtgcattt tacgatgttt tccaattaga
     1021 gctccatttt tatcaaatac atagcaagta ttatagattt ttccatcttc taactcagga
     1081 atactaccag aaactagata aattccatgt ttctcagcta atttacttaa tatcgttgtt
     1141 gtttcacctg gatattcttc agcaaattta ggaaaatatt tattttcata tgggcagtta
     1201 aacatttctg gcaaaactac aattttagct ccattgatgg cagcttctgt taccatatct
     1261 acagcatgat tgatattttc aattttattt tctgtagtaa tcatctgaca tactgcaatt
     1321 ttatagaaat tcatagaaat tactcctttc aatattttta tgttattata acatattaaa
     1381 taaaaattag atattcttag atttacctaa atgggtatgg agagaagtga ataagaatat
     1441 taagaggtat ataaaagtta tttttattat agatggaacc catatattga ctttttttat
     1501 atatgttgat agtattatta tagataaaat agaataattg atagagtttt atagggttcc
     1561 gcaattatta atgattggtc tggtccaaga taaaacccac aacatagttt ttgttgtgaa
     1621 cacggaagga caaaaacctg ggagatattt tataatattt ctcaggttat tttattggaa
     1681 aaataagaga tatatttgaa aagagggatg agtttatgaa atggttattt ttgtttgttg
     1741 caggtttgtt tgaagtgttt tgggcttttc aactaaaaaa ttcacatgga ttttctaaat
     1801 taactccaag tatacttact gttgttggaa tgattgtaag tttttatttt ttgtcattag
     1861 cactaaaaca tcttccactt ggaacttcat atgcaatatg gactggaatt ggtactgttg
     1921 gtacagctat tgtaggcatg gctatcttaa atgagcctat tagtatatca agaattgcat
     1981 gtataggctt tattgtggtg ggaattgtag ggttaaagct attatctact aattagggta
     2041 tattaattta aataagctat gaaattaaag gcgagaaaat gtttaaaaat aatcaacatt
     2101 taaaaagtct ttacttcata gcttatctta tttataagtt tattatattt gaaaattata
     2161 tgtatatgct atttatctaa taaaagagca gttaaatcca tagggtcaac tattacacca
     2221 tctttataaa cacgcatatt tccagatgct atttcatcta ttaagattac ttcatcattg
     2281 tagaatccaa attcaaattt gatatcataa agttcaagac ctttttcagc tatagtatca
     2341 cgaactatgt tagaaatttt ttgagtcatg cttttcattg aatcaaattg agcttgagtc
     2401 ataatgttta aagcttctaa accttcagat gttataagag ggtcacaacg gtcgtcatct
     2461 tttaaagttg cttctacgta acaatctagt ttagcacctt cttcaacaca tgaaccataa
     2521 cgacgtaaga aactaccaac agctctataa cgacaaataa cttctaatcc attaccaaaa
     2581 acttttgctg gtagaacttc catagttgct tcttctaaat tagcacttac ataatgagtt
     2641 ttaattccag cattgtttag aatttcaaag aacttaacag acgtctctaa attagctcta
     2701 ccaattccat ctatacttag acctacacta ttttcgcctg ggtcaaatac tccatcttta
     2761 ccagttacat cgtctttaaa tttaagtaag taatttccat tatctaattc aaaaacatct
     2821 ttagtctttc cttgatacac ttttttcata aacaacctcc aaaaagttaa attattatat
     2881 tgaataaggc attagtgtca aaaaagttct gatattcgca tgaaaattta tattgatacc
     2941 ttatatttaa tattatatca caaatagaag gggaatttcc gaacaatttt ataaaaataa
     3001 caaaaaaagt acattttata aaacttataa taagagaact agtataaaag gttagtgtcg
     3061 tttatattga cacacataag ttttaatata agaaactttt gacacacttt aacacacatt
     3121 taatgatagt tttataaaat gcatgaatac aatacttaca tatattggca tgaaagttgc
     3181 tttagatatt taacatataa acaataagta tatattctca aataaaaagt aggggtggtt
     3241 aatagtgaag tcagttagat tttatggtat aagagacaca agagtggagg atgtggatgt
     3301 accaaaaata ttagaaaaag atgatgtaat aattaaagta aaagtagctg gaatatgtgg
     3361 ttctgatata tcaaaatatt caaagacagg tccacatatg gttggagaaa ttttaggaca
     3421 tgaattttct ggagaagtgg cacaggttgg taaagaagta aagagtttta aaataggaga
     3481 tagagttgct gtttgtccag ccatgccatg ttttgaatgt gatgaatgta aaaaaggatt
     3541 gtattcaagg tgtaataatg tagcaataat aggaaataaa gaacttggag gttgttttgc
     3601 agagtataca aaggtgaaag agagaaattt aataaaaata ccagatgaga taagttatga
     3661 gactgccgct gcactagaac cagtatgtat agcaggacat ggtcttttta ggtcagaagc
     3721 taaagttgga gatactgttg tagtacttgg tacaggccca ataggattat tttcaataca
     3781 atgggcaaag atatttggtt ctacaaaaat aatagctgta gatgtatttg atgagaagtt
     3841 agacttggca aaagagttag gggcagatat ttgtataaat gcaaaagaaa agaatattgt
     3901 agaagaaata aaaaggttaa ctgatggaga tggtgcagat attgttattg aatcggctgg
     3961 tactccaatg acatgtggtc aagtactgtt gctaactaaa aagggaggga cagtattgta
     4021 tgcaggtgta ccatatgggg atgtagcttt gactagagag caatttgaaa agatagtaag
     4081 aagtgaatta actgtaaagg gaacttggtt tggaaactca tttccattcc caggaaaaga
     4141 atggagtgct ggattgtatc atatgcaaaa gggtgatatg aatgttgaaa aattagtaac
     4201 acatagaata aatttagaag aagtaccagc ttattttgaa aaagtataca aaagggatat
     4261 tttctttggt aagataatga ttaatataga taattagatt agagtactaa ataataatcc
     4321 atataaaaaa ggttaagaaa ggtatgattc atatggcagg aataattata gccacacatg
     4381 gaaatatggc taaagcaatg ttagaaagtg ctgaattaat agtgggaaaa caggaaaatg
     4441 tagaaacttt aggattaaac tatggtgaca gcatagatga gtttagtaga aggttagaag
     4501 atggaattga gaaatataag gatgaagggg ttttagtatt gcttgatttt tatggcggca
     4561 ctccatttaa tacatctgct attataataa ataaatttgc taaggtatgt gagttagaat
     4621 gtttgacagg tgtaaattta cctatgcttt tagagttatt tttaaacaga gatactatga
     4681 agttaaaaga cttgaagaat ttatgtgagg aagttgggat atcaggaatc aaagatgtta
     4741 aaactgtttt aaagttgtaa aataaattaa aaatttggag gaattattat gagtaatata
     4801 gcattaacaa gaattgatga tagattaata catgggcaag ttattactgc ttggtgtaag
     4861 attacatcag caaaaaggat agtaatcgtg gatgatttag tagtaaagga cccatttata
     4921 gttcaagttt tgcaaatggc agctccatca actgttaagg ttgaagtaca tgatgtagaa
     4981 tcaggcgctg aagttttaaa atcacataat ggagatgaaa atttaatagt attggtcaaa
     5041 tatcctaaaa ctgtcttagg tttagtaaat agtggggtag atttaaagga attgaatgtt
     5101 ggaggtatgg gagctgggct tggtagaaaa agtttttata aaaatatatc tgtgtcagat
     5161 gaagaaaaag aaatatttag agagctaata tcaaaagatg ttaaatgttt tatacaaata
     5221 gtaccagatg caaagaaaat tgatgtagga acattattat aaagaagact atcttgtaaa
     5281 tctttaaaag aaaataggga ggaagaatat gcacatttca ctaattcaag cagtattaat
     5341 agcaattttt tattatcttt cttggagtcc gtggttgact tatgttggat tttttacatg
     5401 gaatagacct ctattggctg gatttgtaac gggaattata ttaggagacc ctgttcaagg
     5461 agcaattata ggagcaggta taaatatgat atatctaggt tttatatcag cagggggagc
     5521 tcaaatggga gacccagctt ttgcaggata tgtaggtact gctttagcta tagcatctaa
     5581 gctagatgta agtactgcaa tggcaatagc tgtaccattg ggaactgttg caactgtact
     5641 ttggattgga aagatgacag taaattcttt ttttgcacat tgggctgata gagaggttca
     5701 aaaagggaat gttgatagag tagcatttat aaatatagta ccaccacaga tactgttatt
     5761 tgcaatgagt tttataccag cattattagt tgtttatttt ggtccaaatg cgatagatgg
     5821 tatgttggaa gtaatgaatg agaatgtgtt acatgtattt aatgtaattg gggctatgtt
     5881 gccagcactt gggattgcta tgaatttaaa attgattgga aacaagttta ctatgccctt
     5941 tttcatatta ggaatactaa tggctgttta ttttaaagta gatataatag taatatctgt
     6001 tattggagta atacttgctc ttacaattac ttcaattaag tacggaaaag atagtgctac
     6061 atcttaaata catgtgaggg ggaaaatatt atgtcagata taaaaaatga agtatctaag
     6121 aaaacgttaa gcaagaaaga tgtaataaaa tcatggttaa gatggttttt ctttgcacaa
     6181 tctaattaca attatgaaag attgcaatca acagcttttt cacattcaat gttgccagta
     6241 cttaaaaaat tatatccaga taaggaagag ttaaaacaag aagtggagac tcatctatca
     6301 ttttttaaca cagaacctat atgtggatgt gttatacatg gaataacaat agctatggaa
     6361 gaagaaaaag ctaatggagc agatgaaata agtggagatg gtatgaatgc tatcaaaaca
     6421 ggcttgatgg gtccactagc aggaattggt gatactttaa ctcaaggagt tataactcct
     6481 atagtgctag ctgtttgtat tggtttaact gagggaggag catcaattgc aggccctata
     6541 ttatttgtaa tagctcaata tataataatg acttcaattt cttttggaat gtggacaaat
     6601 ggatataagt atgggaaaaa agctgttgaa agtatacttc aaggtggaat agtaaataag
     6661 gttatagaag gggcttctat attaggtact ttagtcatgg ggggacttgt aggtaggttt
     6721 attaatttat ctacaccaat aagttatact agtggtgatt ttaaatttag tcttcaaaca
     6781 gatttacttg ataagatatt tccaggatta attcctttgg tacttacttt attagtactc
     6841 tttgctttaa aaaaaggtct ttctccaata aaaataatgt ttatactgat agtagtggga
     6901 gctattacag gtattttagg actattttaa ataaatatat attggtgtaa gttaggattc
     6961 tatatctatt gattatattt gtaggatgtt ggaaaattta tattaaaaag tttggcaatt
     7021 agggtaattt tataatagat taaattttaa acttatgtaa gctatatgta gtatttaata
     7081 ttttaaaaag tatctcagaa agatttttaa tcagaatgag gtgctttttc atttttatat
     7141 aaaatattaa gtaatttcaa aaaaatataa atttatttaa tttaattaa
//
LOCUS       DAEMAE010000188         7168 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEMAE010000188 DAEMAE010000000
VERSION     DAEMAE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.190"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..897
                     /locus_tag="KPH93_002994"
     CDS             58..897
                     /locus_tag="KPH93_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG1689769.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            1165..2646
                     /locus_tag="KPH93_002995"
     CDS             1165..2646
                     /locus_tag="KPH93_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1689770.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRALEYEINDIM
                     TVTITGWIILSFLFLGIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIANIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            2679..6116
                     /locus_tag="KPH93_002996"
     CDS             2679..6116
                     /locus_tag="KPH93_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG1689771.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSNYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFMSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIILIIYIIYKNNKSEKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVKRIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGEEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYTNAGYLSMIGYTREQLEKELKYKACNLIFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNENW
                     QKPFGIKPDNNYSQMVKIIANSIVHKDNKEIFLNTFLCKKLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIHSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIHQNELENNISDNNIKLADK
                     YEESKPVLKEILDSIDNYIYIVNQNTYELLFVNNKVSRLDLDIKLGETCYKSLLKFDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            6134..6880
                     /locus_tag="KPH93_002997"
     CDS             6134..6880
                     /locus_tag="KPH93_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG1689772.1"
                     /translation="MLNSITLILGIIFTTYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVATESLIIFYPKNSLESKSDYLLILGASVKK
                     TTLSTTLKGRLDTALKYLKIDDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHNERKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIVFDR"
     gene            complement(6829..7149)
                     /locus_tag="KPH93_002998"
                     /pseudo
     CDS             complement(6829..7149)
                     /locus_tag="KPH93_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
BASE COUNT         2991 a          681 c         1113 g         2383 t
ORIGIN      
        1 cagtgaaaat tttaaatttg agtttaaaga aaaataatct agggggaagt aaaaataatg
       61 aatattgtta ttgtaggact aggagttata ggaggctctt ttgctaaagc attaaaaaaa
      121 gctggatatg aaaatgtatt tggagtagat gtagatttag aaactttaaa aaaagcagaa
      181 aaagcaaaaa tcataaaaaa aggatgtacg acaggtaaag agttatttaa aaaagctgat
      241 ttaattatac tatctatata tcctagatta gttgtagatt ttctaaataa taataaaaac
      301 ttttttaaaa aaggaactat aattactgat actacaggaa taaaagaaac acttatcaat
      361 gatgtgttgc aaattattcc agatgatata gattttatat ttggacatcc aatggcaggt
      421 agggaaaaaa aggggattga ttttgcaagt gaacaagttt ttaatggcgc aaattatata
      481 attactccaa ctggcagaaa caacataaaa aatttagagt tagttgaaaa tttaatttta
      541 gagattggat ttaaaagagt aaaaaagtta acttcacaaa aacatgatga gattatagct
      601 tttacttctc agttacctca tgttatggca gttgctctta taaatagtga tgaagaaggt
      661 agagatacgg gtaaatttat tggagatagt tatagagatt taactagaat tgcaaatatg
      721 aatgaagact tatggagtga acttttttta ggaaatagag ataatttact aaaagttata
      781 gaaaactttg aatcagaagt aaatttagta aaagaagcta tatttaacaa tgataaaaat
      841 aaattgatag agtattttaa aaaatcatca ataagaagag aagctttaga aaaataaata
      901 ttaatattta agctatatat agatgggtat aaatataagc gatagtgtat ttaattgtat
      961 taataaatat ggattcaata taattaaata tgctattgat atgtttgtac ccttaagtat
     1021 tgttatcttt taaatgataa tgatatttat ttaaatgctc tttattaaca atttataaaa
     1081 cttaattgta aaaaatactt attaatagta aaataaagtg aatcaattat attataattg
     1141 ctaaagagag tgaggtgtag ctatttgaaa aaaaatagat taatagcaaa gaatatatca
     1201 atagcattta tagtactatt cttttttagt gtatttacat tcttttatgt aggtaatatt
     1261 aatagagcgt tagaatatga aataaatgac ataatgacag ttacaattac tggatggatt
     1321 atactttcat ttttattttt aggtattata atttacatat tatattcaaa agcaaagagt
     1381 caaaaaacta tagaaaaagt agcatatacg gattttgtaa caggctattc caactggaga
     1441 aagtttgaat tagatgttac aaatttatta aagaaaacat cacaaaataa taaatatgca
     1501 atggtcatat tcgatattga taaatttaaa gcaattaatg atatatatgg ccataaaaag
     1561 ggaaatttaa tattaaaaga tattgcagat actttaaatg aattgactaa tatcaatgaa
     1621 acttttgctc gtgtaagtgc tgataatttt aatatattat taacatataa caaaaaagag
     1681 gatataataa atattatcaa aaagattatg gcaaataatg aacttgtaaa cttatctttt
     1741 ggaatttatg aaattaaaga taaggattta tctgtaagtg tatatagtga tagagcttca
     1801 cttgcaaaat catcaataaa aaataatagt gatgtaaatt ttgcattttt taatgataaa
     1861 ttaagagaga aattattact tgaggataag atagaaaaag aaatggaata tgctcttgaa
     1921 agtggacaat tcgtaatgta tttacaacca aaatataata ttaaattaga taaattttgt
     1981 ggaagtgaag ccctagtcag atggcaatat acagaaaaag aggtcatata tccaggtgat
     2041 tttattccta tatttgaaaa aaatggattt attagaaaaa ttgatatgta catattagaa
     2101 caagcttgta aagaaatccg gtctttattt gataaaggaa tttcaccttt acctatatct
     2161 gtaaacttct caagagttga ttttttaaaa aaagatttta ttgcaaacat agtaaatata
     2221 tgtgatagat ataaaatacc atatagttta atagaaattg aaattactga atctagtatg
     2281 tttggagata ctgatactct atttaatgtt agtagaaacc tacaggatat tggctttata
     2341 gttgcaatgg atgattttgg ctcaggatat tcttctgtaa atatgcttaa aaatatacca
     2401 ttaaatgtaa taaaattaga tagaggattt tttgttgatg ataaagatgt tgataagagt
     2461 caaattgtta taaaaagcat agtttctctc attaagcaat taggaataag ggttgttgct
     2521 gaaggaattg aaacaagaag tcaaatagaa atgttaaaaa aagcaaattg tgatattgtt
     2581 caaggatatt atttttcaaa accattacca attaaagaat ttgaaaaatt agtctataaa
     2641 atatagtcag cgagtcttaa atgggaggtg gtgattaagt gataagagca aaatcgttaa
     2701 aaaatgtact gagtattgtt ttaatatttt tatttacaat aatttctttt tctgattttg
     2761 catttaaaac taatgtccaa gaagaagaaa atagtaaaat taatttaagg gaaatatccc
     2821 tacaaagttc caatgctata aaaataaaat tagatagttc tctatccttt attaaaagtg
     2881 tggcaaatat ggtttcttat tttaatacta tagataactc aaaaatagtt ccattattag
     2941 ctaatatatc aaaagaatct gattttcaaa gaatctctat tgtatctcct gatggaataa
     3001 gtcataccag cgatggcaaa attatcaatg tgtcaaatta tcaatatttt aagaaagcta
     3061 ttaaaggaga aagtaatata tctgaagttg taaagtctcc aattgatgga aaagatactt
     3121 ttgttatagc tactcctgta tttaataaga ataacaaaat aattggtgtt ttatacggtt
     3181 cttactataa tgataaatta gctgaattta tagatataac aagttttaat agtgaagggt
     3241 acatagatat atttgaatct agtggccaat ttgttttaaa atcttcacat acaaacaagc
     3301 tgattaagtc aagtagtaat atatatgata tatttaaaaa agcttctttt gatgatggtt
     3361 atagctataa aaaaatatat gaagatacaa aaaataacaa gagtggcttt atgtcatatg
     3421 aatacaaagg agaaaatcgt tatgctactt atacacctat tggtgtaaat gactggtatg
     3481 taatttctgt gataccacaa tatactgtaa gaagaaacat tgataaaata aatcaattag
     3541 cattttctct tattttcaag gtattcttat tgtttattat acttataata tatattattt
     3601 ataaaaacaa taaatctgag aaagatatac taaaagctaa caaagaaatc tcatctatta
     3661 caaatagcat tcctggagga gtacaaaagt gtaccctggg tgaaacatgc aatattaatt
     3721 ttttaagtga tggatttata gagttgtttg gttatacaag agaagaaata aaaacattgt
     3781 ttaataatga tttttatagt ttaatacata aagatgatgt taaaagaata aaaaaagaaa
     3841 tttctaaaca actttacaaa aataaaacta ttgaattaga atataggatt ataacaaaaa
     3901 atggtaatac agtatgggtt ttagataagg gagagcttat aacagaacct gatggagaag
     3961 agtatttttg ttgtgtaact gtagatatta ctagaacaaa acaattagaa caagagatgg
     4021 aacaaaaaaa tctagaactt gaaatgatat cagaaaatgt tttaggtggt ataattataa
     4081 cagaatttaa tgatgatttt actataaaat acactaacgc tggttatctt tcaatgattg
     4141 gatatacacg tgaacaatta gaaaaggaac ttaaatacaa agcttgtaat cttatttttt
     4201 atgaggatag aaagaaggct cttgaaagta tacaacatca attaaacaca aaaggaatag
     4261 ctgtatatca acatagaata agaagaagag atggtaatat tatctgggtt tctataaaag
     4321 gaaggcttat tattgatgat gatggtattg aaaaaggtat ttggattcta aatgacataa
     4381 cagatttaaa aaatgcagaa caaaaactta aaataaatga agaaagattt agaatcgcat
     4441 taaagcaaac atcaaacact atatttgagt atgatataaa aacaaagtca cttttaaata
     4501 ctagtaaaga gcttgataaa tataatattt ataaaattac taaaaatgtt ccatattctc
     4561 tagtagaatc aggagttatt cataaagatt ttagtgacga ttttataaat atgtatgaaa
     4621 atgtgataaa tggagcaaaa aaagcaagtt gtgtagttaa gatgaaactt ccagatgaaa
     4681 attatatatg gaataaagtt accttgacta ctatttttga tgaagatggt aatcctgtaa
     4741 gagcaattgg tatacaggaa gatataagca aacaaaagga agcagaaata agatacaatc
     4801 aagaaggtaa gtatcattca gctatattgt cggaagcaat gataacttat gaaataaatg
     4861 tatcaaagga tacttttatc aaaggaaatg aaaattggca aaaaccattt ggaattaagc
     4921 ctgacaataa ctattctcaa atggtaaaaa ttatagcaaa tagtattgta cataaagata
     4981 ataaagaaat atttttaaat acatttttat gtaaaaaatt attggatgca tataaaaata
     5041 atgtaactga aataaaatta gaatatcgaa gaatgaatca agaaaataaa atgatttggg
     5101 tcatttgtac aatgcactta ataaaagatt ctattacgaa cgatataaaa gcacttgctt
     5161 atgttaaaga tattgacaag caaaaaaatg aagaaattag attaaaatta aaagctgaaa
     5221 gagatactct tacaggatta tataataaag gtactacaga aaaattgatt aatgatttct
     5281 tgaactctga ggaagcaagc ttaggaatgc atgcattttt aattgttgat atagataatt
     5341 ttaaatctgt gaatgataat ttaggacatg tatttggaga taaagtatta tttgaagttt
     5401 ctaaacacct aaaacctata tttagaaaag atgatatagt aggtcgaatt ggtggagatg
     5461 aatttatagt attcttaaaa aatattcatt cttttgagaa taccaaaaag aaagcagaaa
     5521 aaatatgcga tatatttaga aattcttata cggaaaataa aaagacatat aaaatatctg
     5581 gaacagttgg aatttcattt gtaccagaac atggaactgt atttgaagaa ctttataaaa
     5641 aagcagacat agcactttat tatgcaaaaa gcaaaggaaa agataagtat gcactttata
     5701 atgattgtat ccaccaaaat gaacttgaaa ataatataag tgataataat ataaaattag
     5761 cagataaata tgaggaatcc aaaccagtac ttaaagaaat tcttgattct attgataatt
     5821 atatatatat agtgaatcaa aatacttatg aacttctttt tgtcaataat aaggtgtctc
     5881 gtttggattt agatataaaa ttaggagaaa cctgttataa atcactatta aaatttgata
     5941 gaccatgtaa taattgtcct cttaacggat tagaagaaaa taagcattca aaattcaaaa
     6001 tagagacaaa tacatttggt aaaaatatca gtatagcaag ttggattaat tgcatagatg
     6061 gagaatataa ctgtcttata gagtgtatag attcttcttt atacaaaaat aaataaaaaa
     6121 ggactaagaa ggtatgttaa atagtataac tttgatactt ggtataatat ttacaactta
     6181 ctatttttat ctcaaaatag tttttggagg aatttcattt agtgagattt tcttagttgt
     6241 gggaattgta ctaattatct atcaaatatt taagaaaaaa atcaaagaaa agaaggtttt
     6301 ttatagagtg ttaaaaataa taatcagcat ttttttaata gtatttgtag ctactgagag
     6361 tctaattatt ttttatccta aaaacagttt agaatcaaaa agtgattatc tcttaatttt
     6421 aggagcttct gtaaaaaaga ctacactaag tacaaccctg aagggtaggt tagacactgc
     6481 attgaaatac ctaaaaattg atgataattg ttatgtggta gtatctggtg gtaaaggaag
     6541 tggagaaaaa ataactgagg caaaagctat gaaagattac cttattaaaa atggcataga
     6601 taagaatagg ataatagaag aggataaatc cactaatact tatgagaatt ttaaatattc
     6661 aaaattgaag atagaagaac ataatgaaag aaaactaagt gatttaaagg taaaaatagt
     6721 aacaacagat tttcatgttc ttaggagcaa aatattagca tatagaaatg gatataaaaa
     6781 tacgtctttt tatgctagta aatcaaaact ttcatttgta ccaacttact atacaagaga
     6841 attttttgca ttatggaaaa caatcgtttt tgatagataa taaataaaaa gactagatat
     6901 aaactcatat ctagtctttt ctattccctt aattctaatc cattttccat ctttacttgc
     6961 tgtagaaatt tcaatattgg gatttacaag taattgctta taagaagttt tatgttttcc
     7021 gattccaaaa tataattttc attcataatc cattacaaat ccgaatggtc ttactcttgg
     7081 tttgttgtat tcaactgtag caaaatagac tgttgagttt tcttttaaaa aattcattat
     7141 ttttttcatg attaaatcct ctttgtat
//
LOCUS       DAEMAE010000189         7088 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEMAE010000189 DAEMAE010000000
VERSION     DAEMAE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.191"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            118..999
                     /locus_tag="KPH93_002999"
     CDS             118..999
                     /locus_tag="KPH93_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG1689773.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPSRSLNLDTFVNELNKTDEAMKKALGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1060..2268)
                     /locus_tag="KPH93_003000"
     CDS             complement(1060..2268)
                     /locus_tag="KPH93_003000"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG1689774.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAREAEERFKSVFQKGQIPEDIQTIQ
                     VEEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2341..2593)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2745..3734)
                     /locus_tag="KPH93_003001"
     CDS             complement(2745..3734)
                     /locus_tag="KPH93_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG1689775.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLWNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKIDIRLSQSNIVNKQINLLIEK"
     gene            3886..4326
                     /locus_tag="KPH93_003002"
     CDS             3886..4326
                     /locus_tag="KPH93_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689776.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4653..4880
                     /locus_tag="KPH93_003003"
     CDS             4653..4880
                     /locus_tag="KPH93_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1689777.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4906..7026
                     /gene="feoB"
                     /locus_tag="KPH93_003004"
     CDS             4906..7026
                     /gene="feoB"
                     /locus_tag="KPH93_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG1689778.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFIIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIEDVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
BASE COUNT         2521 a         1024 c          982 g         2561 t
ORIGIN      
        1 ataagttaca ctttagttat cttagttaca actttgtcgg tttacttttt ctactttctt
       61 cgatataatt ataatatatt tttgatacaa ttcgacaaaa gggggaatgc tgtaattatg
      121 gtaaaaaaaa gaaaaaagaa aactgttaca cttactatac ttgttgtaat agttattggt
      181 attttttcta tggcatgggc ggacatgtct agaaaaaaag aaatgaaaga aactcaagaa
      241 agacttaaag ctgaaagatt agaaaaagaa agaattgaaa aagaaaaaaa tgaacctatt
      301 attggtgcaa aaaaagaagc tgtaaaagaa tatggatatg acgcaaaaat agtttgggac
      361 aaacttaata aatatgacta ttcaaacaat ggagaaaaga tagtctttct tacatttgat
      421 gatggaccat ctactactaa tactccacaa gttctagata tcttaaaaag acatggtgtt
      481 agaggaactt tctttattaa gggtgattct cttgaaagaa aaggagctaa tgaaatttta
      541 aaaagaacat ttgacgaagg aaatgcaata gcccatcact cttatactca tgactataaa
      601 aaactatatc ctagcagaag cctaaatctc gatacattcg taaatgaact taacaaaact
      661 gatgaagcta tgaaaaaagc cttaggaaag aatttttcct caaatgtagt tagatgccca
      721 ggcgggtata tgtcatggaa aaatatggag cccctaggaa actacttaaa agagaaaaac
      781 atggcatcaa ttgattggaa tgctttaaat gctgatgcag aaggtaaaaa gaaaaatgca
      841 caagaattat ttgaacatgc aaagaaatct tctgaaggca aagaaatggt tgttctttta
      901 atgcatgata cttatggaaa acaagaaaca gtaaatgcat tggaccaaat tataacatat
      961 tttaaagaca atggttatca atttaaaatt ttaatataat aaaaaagcaa gtttattgaa
     1021 tatatatttt atactcaata gacttgcttt tactttactc tattttacta gctcgatttt
     1081 tacaaatttt ttctttccta tttgaacaac taattcactc tcttttacaa tagtgcttaa
     1141 atcctctact ttttcaccat ttacctttac tccaccttga gaagctaatc ttctaacttc
     1201 actcttactc ttaacaatct cattagaaac tagtacttcg attaagtcaa atccatcttc
     1261 ctcaacttga attgtttgta tatcttcagg tatttgacct ttttggaata cagatttaaa
     1321 tctttcttca gcttctctag cagattcttc tccatggtat aatgttacta tttctcttgc
     1381 taagttcatc tttatatctc ttggatttac tgaaccctct ttcaactgag attctatttt
     1441 attaacttca tcaggatgaa catctgttac taaattgtag tatttaatta taagttcatc
     1501 aggaatttcc atagattttt ggtacattat tcccgcttct tcatctattc caatatagtt
     1561 acctaaactc ttactcattt tttctttacc atctaatcct tctattaaag gcatcattat
     1621 tactatttga gactccatac caaattctct ttgtaatgat cttcccatta gtaaattgaa
     1681 tctttgatca gttccaccaa gttctatatc agcttctaat gctatagagt catatgcttg
     1741 cattaatggg tagaagaact catgtactga tattggcatt tgtccttcat atctcttttt
     1801 aaaatcttct ctttctaaca ttctcgcaac agtaatagtt gctgctaatt ttattacatc
     1861 ttcaaaattt aacttggcta accactcgct attaaatctt acaatagttt tttctttatc
     1921 taaaactttg aaaatttgtt cttcataagt ttttgcattt gcaagaactt gctctgttgt
     1981 taaagctttt ctagctttag attttccagt tgggtctcca atttttcctg taaaatctcc
     2041 aattattatt actatttgat gacctaaatc ttgtaattgt ttcatctttc taagaactac
     2101 tgtatgccct aaatgtatat ctggagcact tgggtctaga cccaatttta ctatcatagg
     2161 ctttccttct ttttctgatt taataagctt ttcccttaac tctttctcat ctattaaatc
     2221 atctacacct ttcataatta ttcgcatttg ctcgtctatc gatttcattt ttcattcctc
     2281 ctaaaatatt tatataatct ataattttta tttttataat aaaaaaagct ttcatcctct
     2341 taaaaggacg aaagcattgc ttacgtgtta ccaccttagt tcatcaatat ctcacgatat
     2401 taatctctta gagtacaatt acatatactc gtgtactata acgtgtacta atccgatatc
     2461 tcttaatggt tgttatcttt cagagatagt gctctgagat gtattcaaac taaatcaatt
     2521 tattccattt caccaaactg gaactctctg ttaaataaaa tctagcccta cttcttctct
     2581 tcatagcatt tatatattca gattatttca tcaattacaa gaaatttttc ttaacttact
     2641 cataatatat tattattttt atattttgtc aacctttttt attattatat acatgttttt
     2701 ttacattata ctactttttt atatttttta ccaataccta aagtttattt ttcaatcaaa
     2761 agatttattt gtttattgac tatattactc tgactcaatc ttatatctat tttcttattt
     2821 aaaaatgcgt ttataattat taaactaata actgttacat caaatataaa aaacagcaat
     2881 aaaattatta tcaaaaataa aattctagca tataataatt ttctaaactt agctattata
     2941 aagttttgta gtgacataca tgagtctcta ataataaaat agacacttat ataggttatt
     3001 gatataaaga ttgtttttaa ataaacagaa tcaactttaa cattccacag ttttaaaact
     3061 tcaaaaatgg ctagataaga aacacatatg taaaaagctt tttttgagca acataaaaat
     3121 tggtttaaac ttctaaaatt cttaaatatt aaaaattttt ttaaagaatt agaatctaaa
     3181 atatgtaatt tattccctaa aattaaactt aataaatata tgcaaaacac cataccaaaa
     3241 ggaaacaatg ctatcattaa ataataaata aaatttgtat acccattaaa ataaatccat
     3301 ttgctatatc cttgaaattc tttcataaaa ctataaccta ttaatgttga agcatatata
     3361 tcaatgaaaa ccattacaat aaccccaaat actgaaccat atactaaatc atccataacc
     3421 attgtaggct tatttattag atagccacac attataccaa gtaatacact ctgacttgcc
     3481 caaatagcag ttcctagatt tccaagtacc agagaaatta ttaaaagcga agtaattcca
     3541 gataaaatgg tatactttaa atcacatttc aagcagatta tacaaaaaaa tactggcact
     3601 ataaaatcta agtaaatagc atatccaaat ccagtactca cagctataat tgttactact
     3661 atgaataaag atgacaataa agctgcttca gttaattttt tagtagtatt caaaacactg
     3721 cctccataaa tcataaattt tctaactaaa gttttgtttt taatatagta tatcactttt
     3781 taaaaatatt tttaataaat ataaatttta ataaaggtat ttatgttaaa aatggtataa
     3841 tgtatcttaa tattcagttt tttaagatgc taggaggtaa ttactatgtt taaaattaaa
     3901 cactttaatg atttgagttt agatgaattt tatgaaatag caaaatcaag atatgaggta
     3961 tttgcttgtg aacagaagat attttcatta aacgattatg atgatataga taaaagctct
     4021 tatcacatat ttttaaaaga aaatggattg atttgtgctt atgctagaat aattcctaaa
     4081 gaatacagct cttataatga tgtttccatt ggtagagttt tagttctttc tagtcataga
     4141 agaaaaggct tagcaaaaca aatgatggat tgtgctatag attttataaa agttaactta
     4201 cacgaaaata atataacctt atcagcacaa acttatataa agaaccttta cttatcttgt
     4261 ggttttaaag aaatctcaga agtatacgac gaagcaggta ttgaacatat taaaatgaga
     4321 ttatagtgtt gacaagctaa tattgttaat atataattta agtataaatt aaatattgaa
     4381 ttgatattta ggtatttgaa aataccttac atagagtcag gaggcttttg ctttctgact
     4441 tttttattat caaaattcaa taattatatg gattcaaagt gttaattttt attctatatt
     4501 ctactttccc caaaagtaag aattataccc aactttgtat gataggcatc ttgcctatct
     4561 tttttttgta aaagtaacaa ggtaataata taatgcatag tttagaataa gaagattagc
     4621 ccaggctaaa tttcaagcat ggaggtgcta ctatgaaaaa ccttaacgat attcaagtga
     4681 aaaaaactgt caaagttgaa gatatattat ctagtggtaa tttaagagaa agaatgttag
     4741 cgcttggtct tactagagga gctgttatag atgtagttag aaaaggtcca aaaaataatt
     4801 taactgtcta taaaattaga ggtagcaaga tagctctacg acaagaagaa agtagcttaa
     4861 tattagtttc tgacatttaa aaatttctag caggaggata ttaatatggg gttgacacat
     4921 aactcaacta aaatgagttc tttaaaagac atgtttgata tagacaataa ggaagaccag
     4981 tttataattg cattggctgg caatccaaat acaggtaaaa gtactgtatt taatcatctt
     5041 acagggctta gacagcatac tggaaactgg ccaggaaaaa ctgtagctac agctcgtggt
     5101 aattttaaat ataaaaatac cgaatatgca ttaatagatt taccaggaac atattcttta
     5161 tttgccttat cacaagaaga aattgtagct cgtgatttta tatgttttgg taatccagat
     5221 gcagtaatag tagtgtgtga tgctacatgt cttgaaagaa atttaaattt agtatttcaa
     5281 gtgatggaac ttacagataa agttattctg tgtattaatt taatagatga agctagaaaa
     5341 aaaggcatta ctattgataa aaaactttta gaggattctc ttggtattcc agttatttta
     5401 actgctgcaa gaaatggctc tggtatggat gaactattgg atactttaaa tgatgtatct
     5461 tttgacaaat ataaattaaa taataaacct gttagataca atgaaaatat agaggatgtg
     5521 gtaaaatcaa ttcaaccaga attggataat attataccag gtataaattc tagatggttg
     5581 ggacttagac ttattgatgg agatgaaagt atatttgaat ctatgagtaa ttatatagac
     5641 aaagattcta tagatgcgat taatgaagtg aagaaaaaga ttccagataa tataaataaa
     5701 cagaaaataa gagatgagtt tactaaaatc aactatgatt atgctaaaaa actgagtgat
     5761 gagtgttgtt ctaatgttgc taaaaaatca actgatagag aagaaaaagt agataaaata
     5821 ttaacatcta aaatatttgg gcttccaata atgctcttat tattaggtac tatattgtgg
     5881 attactatag aaggggctaa ctatccatcc actttattat ctaatttatt actcggattt
     5941 gagccaagta tttcgggcat attaaatagt attaattgtc catcatggtt aaatgatatg
     6001 ttggttttag gattatacag aacattagca tgggtaatct ctgtaatgct acctcctatg
     6061 gcaatatttt ttccattatt taccttactc gaggattttg gatatttgcc aagagttgca
     6121 tttaacttag accacctatt taaaaaagct tgtgcacatg gaaaacagtg tttgactatg
     6181 tgtatgggat ttggatgtaa tgcagctgga gttattggtt gtaggataat tgactctcca
     6241 agggaaagac ttatagctat acttacaaac aactttgttc cttgtaatgg tcgttttcct
     6301 actttgatag ctatatctac aatatttttt agctcagtta taaccaactc tttcgtatca
     6361 agcgtagcaa ctgcactttg cataacttta ctgataattc taggtgttat aataacattg
     6421 ttggtttctt atactctttc taagacgcta ttaaaaggag ttccgtctac atttacatta
     6481 gaacttcctc cttatagagt tcctcaaata ggaagaactt tatatacctc tataatagat
     6541 agaacaatat ttgttttagg aagggctgta atggtagcca tacctgctgg tgtcataact
     6601 tggatatttg caaatatata tataggagat ttaagtatat tatcacatgt tgcaaacttt
     6661 ttagaccctt tagctaaatt aattggtctt gatggtttta tattacttgc ttttattctt
     6721 ggattcccag caaatgagat agttgtacct atacttttaa tggcttacct agcaactggt
     6781 tctatgatag aattagatag ctttagtgct ttaggtcaag ttcttagaga acatggatgg
     6841 acttatttga cagctttaaa tgttatgctg ttcagtttac tgcattggcc ttgtgcaaca
     6901 acacttttaa caataaaaaa agaaactggt agtttaaaat ggactgcctt aggattctta
     6961 atgcctacta tacttgcttt tgttgtttgt tttttaacaa ctacagttta caatttattt
     7021 atataattag tattaactga cagtagacat atattagaaa aactatttaa aaatttatat
     7081 actttatt
//
LOCUS       DAEMAE010000190         7067 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEMAE010000190 DAEMAE010000000
VERSION     DAEMAE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.192"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            392..901
                     /locus_tag="KPH93_003005"
     CDS             392..901
                     /locus_tag="KPH93_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG1689779.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            929..1123
                     /gene="rpmI"
                     /locus_tag="KPH93_003006"
     CDS             929..1123
                     /gene="rpmI"
                     /locus_tag="KPH93_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG1689780.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1178..1534
                     /gene="rplT"
                     /locus_tag="KPH93_003007"
     CDS             1178..1534
                     /gene="rplT"
                     /locus_tag="KPH93_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG1689781.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1844..2389
                     /locus_tag="KPH93_003008"
     CDS             1844..2389
                     /locus_tag="KPH93_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689782.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIRVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGMIITPPKEDEGK"
     gene            2826..4751
                     /locus_tag="KPH93_003009"
     CDS             2826..4751
                     /locus_tag="KPH93_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1689783.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSIVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIRRNGRAEY
                     DDIIKNSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDGGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEVSFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKNIRDNNSGKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     ENVNLQEQFQYKGKLYANDLVEDAKKWVKVMKKKKTDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVEGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KDSWIINDKFSKTVEVE"
     gene            5146..7050
                     /locus_tag="KPH93_003010"
     CDS             5146..7050
                     /locus_tag="KPH93_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1689784.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGSVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFVDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKSIKMK"
BASE COUNT         2985 a          713 c         1333 g         2036 t
ORIGIN      
        1 gtagtatagt attatattag aatacaatat aggatttgtt atgtagaata tataaagtca
       61 tgtagaatat acctatataa aaggattttg ggtataaatt taaaaattaa tagcaaaatg
      121 agtattatag acatataatt agtagtagga agtaattaaa tttttcctaa acaaaaaaaa
      181 tagttgcaaa taatttgatt aagtgatata gtaaataagg taagaataaa acaaagaaga
      241 agtccgcttc tcaccttacg ccagaggtta gtaaggtaaa tataagttaa agcttaattt
      301 taatttaagt ttgtttaaat ttggtaactt atattgcgga tgattacgtc atccgctttt
      361 tattttataa aaattttgga ggtgtcctac aattagcaaa gaattagcaa tcaatgaaca
      421 aataaaagat aaggaaataa gagtcctttc gccaacaggt gaacaacttg gagtaatgcc
      481 tactaaagaa gctcaagcaa tggctaatag caaaaactta gatttggttc aaatctcgcc
      541 aaatgcaaat ccaccagtat gtaagataat ggattatgga aagtttagat atgagcaagc
      601 gagaaaagag aaagaagcta agaaaaaaca aaaaactata gttgtaaagg aagtaagact
      661 tagaccaggt attgaacaaa atgatttaaa tacgaaagct aataatgcta taaagttcct
      721 taaaaaaggg gataaagtca aagttgaact tagatttaga ggaagagaat taggacataa
      781 agacataggt aaagaagtta tgcttaagtt tttagatata ataaaagagt ttggagaacc
      841 aacaaaagcc cctgcatttg agggtaataa tatggttgta attatagatc caaaaaaata
      901 gatgattaac tgagaggagg aatttgtcat gcctaaaatg aaaactcata gaggtgcagc
      961 aaagagatta aagaaaacag gaactggaaa gttaaagaga gctaaagctt ttaaaaaaca
     1021 tatattaact aaaaaatctg caaaaactaa aatgaactta agaaaatcaa ctttagtaag
     1081 tgatggagac gctaagagaa tagcacaatt attaccatac taagatacag attataataa
     1141 taactataga agagggatat aaaaggaggt cataacgatg gcaagagtta aaaaagctat
     1201 gaacgctcgt aaaaagcata agaaaatatt aaaacttgca aaaggattca gaggatctag
     1261 aagcaaatta tatagaccag caaacacatt cgtaatgaaa gcattaaaaa atgcttatat
     1321 aggaagaaag ttaaagaaga gagacttcag aaaactttgg atacaaagaa taaatgcagc
     1381 tgctagaatg aatggaatat cttattcaag attaatgaat ggattaaaat tatctggtgt
     1441 tgaagttaac agaaaaatgt tatctgaaat ggctatacaa gacccagaag gatttgcaaa
     1501 attagcagaa gttgctaaag caaaattagc ttagtattga atcatgagat gtaaagtctt
     1561 tgagttatat aaataattca aggacttttt tattttttga aaaatactta ttagtgtcat
     1621 gtacaagcaa ttttataatg ataaataaaa taagcaatac aatagaattt tctgatattt
     1681 attataaaaa ttgttacaat tatttatgat agctataaaa agtcatagta aaaatgatat
     1741 tgatatatgt tgatatatgt ataaagtttc caatcaatct tgaattaata ttatcaacat
     1801 gataatatta tcttattaat aatataaata agggggagat aatatgaatg agaaactaat
     1861 ggattgcttt gtaaacccaa ttaaatgcaa attattactt gaaatttttt ccaataatcg
     1921 aactacagct aaacaattag gagaaaaatt tcaagatata tcaaaagcaa cactttatcg
     1981 ttaccttaat aaaatgctta aagatgatat tataagagtg gttgaagaaa ataaaataag
     2041 gggaactgtt gaaaaagtat atgctatgaa gttagatata gctgatgatg tgaacaaaat
     2101 tatagatgag aactctggag aagaatatgc tcaactgttt actcaatata tgatgggatt
     2161 tttaagagaa ttttatgaat atgctgaaag aaaggaaaag gatatcttaa aagatggttc
     2221 aggattttct gtctgtccag tatatgctag tattgatgaa atagttgaag tgagtaaaaa
     2281 aattgctagt atactagaac ctttaagaaa taattcacac gaagaaggta gaagaatgca
     2341 ttctatagga atgataatta ctccacctaa agaggatgaa ggtaaataaa tctgatttac
     2401 atgaggtaga gaccatttat acagggaatc gttttatgat tcacagtatg tatccagagc
     2461 aaaatatctc tgcttagatt atacttcatt ataccatttc atctaatgac ttggagtggt
     2521 acaatgaagt tttttaattt ttcatatcaa taattaaaga aataaacttt agaccacata
     2581 tacaattagg ttacattata caattagacc agatatacaa aataaaaatt agtattttta
     2641 tagcagacaa attgactgtt attcttctta aaaatcatct attagttatt gttagaatat
     2701 tgaaagaaat tatagagacc tattggataa atgaatattg cccatagtca tataaactgc
     2761 tatagtttaa aataaagcag gacgttaaat tttattataa tctaaaaatt taagaggggg
     2821 attttatgaa aataaaattt gttattatgc cattagtgat atttttaatg gtattatttg
     2881 tagggtgtca aagtacaaaa gatacagaag ttaaagcaaa agatacagaa attaaaatat
     2941 taggtacagg tgatttgcat tcaattttga cagactctat agtttcatat gtaaatgagg
     3001 aaagggaaaa gaataaaaat ctgcttatgg tagatgcggg agatttctat ggtacacaaa
     3061 gtagagaaat gtgggaatgg tcttctggaa aaaagttaat taatattaga agaaatggta
     3121 gagcagagta tgatgacata attaagaata gtaaagatga agtacctata gttaaagata
     3181 tggcaaagtt gaaatatgat gctgtagttt tgggagataa tgaatttgtt tcaaatgata
     3241 aacaaagtct tgataaacta gtttctgatt ttaaaaataa taatatgcca ttggtatcag
     3301 caaatatata tgaacaatct ggagaaaatt atgtacaacc atatgtaatg aaaaatatta
     3361 agacagatgg gggcaatgta aaagttggaa tactaggtct tactataaaa gaagtagggg
     3421 aaacccttgg tcttgaagat tttgaaaagg ataaaaaagc aagagagctt ggagagcaag
     3481 ctgattacaa aggaaaatta tatgcaaatg atttagtaga agatgcaaag aaatggataa
     3541 aagttatgga aaaagaaagt ccagacataa tagttgcagt tgtacactca ggggaagaac
     3601 ctaaaacaga tagaaatcca ggaaatagaa taaaagagtt agcaactaca gtggatggaa
     3661 tagatgcaat tgtagctggt catactcatg aaaaaataga tgaacataaa tataaaaata
     3721 aatctggaga tgaagtaatt gtcacacaac caggttggca tggagataga gtgtcagagg
     3781 ttagtttcaa attaaataaa gagaatggaa agtgggacat aaaggaaaag tctagtaaat
     3841 taaaaataat tgataaagaa gtaagtatag ttgctactgc agatttacat tcacatttct
     3901 ctgataaatt aacagttgac cttgttaatg aacgaagtaa cccaattgaa cctgttgtag
     3961 tagatgcagg cgacttctta gaccctcaaa cagatgaaat gactcaatgg tataaagagt
     4021 ggaaaaatat aagagataat aatagtggca aaacgattag tagatgccca atggtcatgg
     4081 atatgaatca gggaatgtat gatgcagtag tacttgggaa tcatgaattt gtatctgaaa
     4141 aagatgaatt ttggtcagat gaacgccttt tagatgctgt tatagaggat tttgaacagg
     4201 tggcaatttc tgtgttatcg gcaaatatat ataggcaatc tggtaaaaat tatgttaaac
     4261 catatattat aaaagatgta gagacgagtg aaggtaatgt aaaagtagga atattgggac
     4321 ttactataaa agaagtaggt aaaggtcttg aaaatgtaaa tttacaagag caatttcaat
     4381 ataagggtaa attgtatgca aatgatttag tagaagatgc aaagaaatgg gtaaaagtta
     4441 tgaaaaagaa aaaaacagat ataatagttg cagttgtaca ttcaggagag gagccaaaaa
     4501 atccaaaaca tccaggaaat agaataaaag agctagcaac tacagtagag ggtattgatg
     4561 caattgttgc tagtcatact catgaaaaaa tagatgagca tgaatataaa aataagtctg
     4621 gagagaaagt tatagtgaca caaccaggtg agcatggtaa atattattct aaaattactt
     4681 ttaaagtgaa taaagaaaag gattcatgga ttattaatga taaattcagt aagacagttg
     4741 aggttgaata actttataaa atttatagat aaaataaatt atatgatatt agtatgatat
     4801 acaagataaa gtttttaata ttagtataat gtgaaatatt ctagcaataa gagaataaac
     4861 aagataaata aaaatatttc aacatgtaag atatagtatg aacatattat actaatgctt
     4921 actttataat ttgagcatgt tagaataagt cttatttgac aattttagtg cactagagta
     4981 agtattattt taccagtaaa aaatattgag aactatatgt agatttgctt aaaaaggaat
     5041 ttacatatag ttcttttcgt ataataaata gtacaatctt atatggtaaa gataaagtgt
     5101 gatattaaat tttattatca tccaaaatct aaggaggagt ctcttatgaa aataaaattt
     5161 attatagtag catcaataat atttttaata gtattacttg tggggtgtca aagtactaaa
     5221 gatacagaaa tcaatgtact cgccacaagt gatttacatt caattattcc agataattta
     5281 atttcctatg tagaagagga gagaaaatat gatgagaatc tacttatggt agatgcagga
     5341 gatttttttg acatgcaaag tggtgagatg aataagtggt ttactggtca aaaactagta
     5401 agatttaaag atgggatacc agagtacaaa aaaatagctg aaccacgtga aggagaagta
     5461 ccattggcta aaaaaatggc aaagctgaaa tatgatgcag ttacatttgg gaatcatgaa
     5521 tttgtatcta atgacaagca gagccttgat aaattggttt ctgattttaa aaataacaat
     5581 ataccattag tatcagcaaa cgtatatgaa caatctggag aaaactacgt aaagccatat
     5641 gtaatgaaaa atataaatac tgacaaggga tctgtaaagg ttggtatact tggtctgact
     5701 ataaaagaag taggggaacg ttcaaggttt aaaagttttg aacaagataa aaaggctaga
     5761 gaacttgggg agcaacctca atacaaaggg aaattatatg caaatgattt agtagaagat
     5821 gcaaataagt gggtaaaggt catggaaaaa aaagaaaagc cagatataat agttgccatt
     5881 gtacactcag gtgaagaacc aaaaaatcct aaaaatccag gaaatagaat aaaagaactt
     5941 gcaacaactg tagagggaat agatgctatt gtagcaggtc atacacatga aaaaatagaa
     6001 cagcatacat ataaaaataa ttctggtgaa gaagtaatag taacacagcc gggagctcat
     6061 ggtaatagta tatcaaagat taattttaag ttggataaga agaatgataa gtggtttata
     6121 aaagacaaat atagtgaatt aaaggttttt ggaaaggaag taaatataat tacaacttct
     6181 ggtctgaatc caaaattttc agatgaaatg gcagttaatt tgtttaatga aagggataga
     6241 gatgttcaac ctatctttgt agatactgga gattttttaa atactaatac aaaagaaatg
     6301 actaagtggg ctaaagaatg gcaatacaat gaaaataaag gtatctataa aaaaataaat
     6361 gaatggccaa ttgtatatct tggttatgat gctgtagtac ttggtagcca tgaatttaca
     6421 gaggataaag agacttttga agaaaaaaaa tctacattgg attatattat agaaaacttt
     6481 gaagaaatga agataccagt attatcagct aatatatatg atgaatctgg agaaaattat
     6541 gtaaaacctt atataattca taatgtagaa actagggaag gaaatataaa ggttggagta
     6601 ttaggtctta ctactactga taaggatgaa gaagacttaa gtagctcaag cttaagcaaa
     6661 gattcaaaga cttcgaaaat tcctgaagaa acaggaaagt tatacactaa caatctagta
     6721 aaagatgcaa gcgaatgggt aaaagttatg caaaaggaaa gtcctgatgt gattgttgca
     6781 gttgtacatt cagataataa tagtactcta aaaaatccaa gtagtgaagt aaaaaaactt
     6841 gcaatgtcag tagatggaat agatgctatt gttgtaggtc atacgaaaaa taaggtagaa
     6901 gaacatattt acaagaataa gtctggggat gaggtcatag taacacaatc tggagaagac
     6961 tgttattcaa aaataagtct tgaactaaga aaagaaaatg gcaagtggaa tatcataaac
     7021 aaatatagta aatcaataaa aatgaagtga atttattgat atataaa
//
LOCUS       DAEMAE010000191         7022 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEMAE010000191 DAEMAE010000000
VERSION     DAEMAE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.193"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            239..889
                     /locus_tag="KPH93_003011"
     CDS             239..889
                     /locus_tag="KPH93_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1689785.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEDV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1191..3257
                     /locus_tag="KPH93_003012"
     CDS             1191..3257
                     /locus_tag="KPH93_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG1689786.1"
                     /translation="MKEVVWSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENAYTSQVYILNNKSGELKQITSINSVGAYAWKDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLSEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYLYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSEHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFASAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3276..5231
                     /locus_tag="KPH93_003013"
     CDS             3276..5231
                     /locus_tag="KPH93_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG1689787.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWVSNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPISLGNKLWLLTTKNPTDSKKSEPDRAVEGIDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENTTKAITPKFCEVASIDVSSDKKRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLSS
                     SEMNMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTSFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQIGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5247..6437
                     /locus_tag="KPH93_003014"
     CDS             5247..6437
                     /locus_tag="KPH93_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG1689788.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVLVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         2636 a          824 c         1327 g         2235 t
ORIGIN      
        1 atctatagta taatagttta atttgaagta caacgattta atctatagta caataacttg
       61 atttgcaata caatgattta atctatagta taatagcatg atttacagta caataattta
      121 aattgtagta caaaagtact atcaaataag aatttctact ttaaaaatat tactaaaagt
      181 aattaaagtc gagtaattca atataatatg gcaaaagaat agaaataagg tgagagtagt
      241 gaaagtaaag tcaaatttag atgaaatttt atatcataag attattgaaa gtcttatccg
      301 tggtgaatac tctgtaggtc aaaaaatatt actaaatgac ctttgtgaaa aatttgaagt
      361 aagcagaaca ccagttgtac aagcagtaaa aatgctaaat aaagatggtg tacttactat
      421 tatgacaaat ggtaaagtgt acgtaccaga gtatgaatat gatatggtaa aacaagtttg
      481 tgaaacgaga acactgattg aaacatatgc tttagaaaaa atgatgcaag aagaggaaga
      541 cgtttttcaa caaaagttag atgtaataaa aagatactct gataaatgtg aggagtacta
      601 tcgtcaagaa aaatctgtag aacttgcact agcagatttg gaattacata aagccattgt
      661 agaaggggca aataatgaga ttttaaatga tgtatatgtt ggtatacaag gaagatttat
      721 agtagtgaac tatttaattc gaccattaaa aaatcgtaat tatgaaggaa cagtacaaga
      781 ccattttgaa atattaaaat tcattgaaga cagagataca aaaaaagcag tagataaatt
      841 aagaaatcat attcaaggaa ctattaaaag attctgtgaa gatgaataat aagttttgaa
      901 gttgctatat atacttatct attaagttgc taaatgtagt ctaagtataa tctaattata
      961 gtttaaacat aatctaaata tagtgtaaat aagtctaagt ataaattaaa tatagattaa
     1021 gtatagacta agtatggatt aagtataaat taagtaccaa ttaagcatgg attaaatata
     1081 ctttaaatta caaatattac aaggaagcca gaaggcttcc taaaaaaata acctttataa
     1141 aattttttat tttaaattta aaatttttta ttttaaattt aaaataaaaa atgaaagagg
     1201 tggtatggag tttgaaaaaa attactaaag aaaggataga gaatactatg aaaaagctat
     1261 ttacgagtga aacatttttg gaatatcaat ttataagtga ttgccagata tcaccagatg
     1321 gaggatatac agcatttatt gtaaaaaaag ctgatatcaa agagaatgct tacacaagcc
     1381 aagtttatat actaaacaat aaaagtggcg aattaaaaca aataacatct ataaactctg
     1441 ttggagcata tgcctggaaa gatgaaaata ctatcttgtt ccctgctttg cgaaatgaga
     1501 aagtaaaaga agctgtcaaa aatggtaaac aatgtatgag ttattatgct ttatctttaa
     1561 atggtggaga agcagaagaa ctttttagac tccctatcaa aggtggtaaa ttgaacccta
     1621 taggaaaagg tttgtatgca gttattgatt cttatgacaa tgaccgacct attgtagagg
     1681 gattgtcaga agaagaacaa cagaaaaaaa taaatgctta taacaaaaga cactatgaac
     1741 atttcaaaga aattccttat gctgtcaatg gggagggata tattagcaga aaaagaaaac
     1801 gcttatatct atacgacagt aatataaatg agttgaaggc aattacagca cctatgttta
     1861 atgttgtagg tatgaaaata agtgatggaa aaatcttata tatagggcaa gagtttaaag
     1921 atgtaaaagg tttgaaaaat ggggtctatg tatttgatac aaacacaaac acaaatgttt
     1981 gtatattaga aaaagataag tatattataa aaggttttga attatatcaa aatcaagtga
     2041 tactaaacct aactgatgct ttatcttatg gaaatggaga aaatggagat ttttatacta
     2101 ttgatattga tactaaagaa atgaaacttc tttcagaaca tcaacatcat tgtattggaa
     2161 atacagtaac atctgatgtt aaaatgggtg ctggtcagac aacaaaggta gatggagaat
     2221 atatttatta cacttctaca gtagatatgg attgtattat tgaaagaata catattccaa
     2281 caggaaaaca ggaaaaagta acacagacag gttcagttga ttttatagat gtaaaagatg
     2341 gcaatattgt atgtgtgggt tgtttaggaa atggattacc agaagtttat acagtagaga
     2401 acggaacatt gtgcaagaaa acacatttaa atgaccatat tttagaggaa tataaaattt
     2461 ctgtgccaga atacatagag tcaaaaggaa gttctaaatg ggaaatacaa ggatacgtat
     2521 ataaaccagt agattatgag gtggggaaaa aatatccagc tgtattggcg attcatggag
     2581 gaccgagatt gacatatgga ccatatttta tgcatgagat acaggtattt gcaagtgcag
     2641 gttactttgt attcttctgt aacccaagag ggtctgaagg tcgtggcaat gcttttgcag
     2701 atattaggaa acagtttgga gatattgatt atatagattt tatggaattt acagatactg
     2761 tacttgaaaa atatacagat attgataaaa caaaattggc tgttgaaggt ggaagttatg
     2821 gtggatttat gacgaattgg ataattggtc atacaaaccg ttttgctgtt gcttgtgctc
     2881 agcgttctat tgcaaactgg agtggcatgg agggtacaac agacattggt tattatttct
     2941 gcaaaggaca aacaggtgct tctcatatgg aaaaccacga gcttcaatgg aaacagtcac
     3001 ctcttgctta tgctgataaa tgtgttacac cgactttatt tttacatggt gaaaaagact
     3061 atagatgcta tatgcaagaa gcattccaga tgttttcagc attgaaaatt catgggtgtc
     3121 ctacaaaact ttgtctattt gaaggggaaa accatgaact atctcgttct ggtagaccaa
     3181 aacaaaagct acaaaggtta gttgaaatgc tagattggtt ttctgtgtac ataaaaaaag
     3241 ataacatagg acagtagaga aaggagaata taaaaatgga aaaaataaag attgaaaatt
     3301 cagatatttt tgaatatata ttccctcatg atatttcttg ttcacctgat ggaaagcata
     3361 ttgcttatat tatatcaaat atcaacgagg aaaaagattg ctatgaacat gacttatatg
     3421 tgatggacat caaaacagaa aaacaaatac atatgactca aacaaaagat gtaacatcat
     3481 ttagttgggt ttctaataca gaactacttt ttacatctaa aagaaataag ccaaaagcag
     3541 gtacaacaga tttttacact atttctatag aaggaggaga agcaaaaaaa gcattctcta
     3601 tcccaaaagc ttgtagtgta cctatatcac ttggaaataa attgtggtta ctgacaacaa
     3661 aaaatccaac ggattctaaa aaatcagaac cagatagggc agtagagggt atagattatt
     3721 ggacatttac agataaacct tttataagag atggagaaaa cttttctcag agaagacgtg
     3781 taacattaga attatatcaa gaaggtgaaa atacaacaaa agccattaca ccaaaatttt
     3841 gtgaagtagc aagtatagac gtttcttctg ataaaaaaag aattttatat actggtcaga
     3901 tttatgaaga ttgtgcaact cctttttctg gactttggga gtatcatata gacagtggtg
     3961 aaaccaaaga acttgtacca caaggaaagt accaaataag tttggcaaaa tatattggca
     4021 aagataaagt aatgttgcaa gcttcaacat tagaccgttc tatcacacag aatcatgata
     4081 tttttatact tgatttgagt tctagtgaaa tgaatatgat tgctagtcca gatggtatgt
     4141 atgctacact actggatgta gatgcagtat atggtggtgg acgtagtaat aaagttatag
     4201 gagataaatt tattggtgca agaatttgca gaactatgac agaatttaat gaatttgata
     4261 caaagacagg taatatacgt atcatcacaa aagtagatgc ttttacatct tttgatattt
     4321 acgacaacac catgtacaca gtaatgttga aagattatga gttagcagaa atatatagca
     4381 ttgatatgac tacaggtaca atgaaaaaaa tgacttcttt ttctaaacca tatttagata
     4441 ctcataaggt ttcattacct gaaaaattga cgtttgtagc aaaaaataaa gaagaagttg
     4501 atggttttgt tataccaccg atagatgcta aagaaggcga aaaatatcct gctgtattgt
     4561 tcattcatgg tggacctaaa tgggcatatg gatatatgtt tacacacttg aagcagtgtg
     4621 taacatcaaa gggaatgtat gtgatttatt gtaaccctca tggtggtgac ggttatggtg
     4681 aaaaattctt agaaatggta gaaagatggg gttatgttga ctatgagcat cttatggaat
     4741 ttgtagatac ttgtattgaa aaatatcctg ggattgatgc agaccgctta ggggttgcag
     4801 ggggaagtta cggtggttat atgacaaatt ggataattgg tcatacagac cgtttcaaag
     4861 cagcagcttc tcaaagaggt attagtaact tgattacagc ttctttaatt attgattttg
     4921 gggatagaat tatgaagcag acttgtggag ataagacacc ttggaatcat gaggaggttc
     4981 tttggaatca ttcacctatt aaatatgtga aaaatgttaa gacaccaaca ttgttcttac
     5041 atagtgatag ggactataga tgttttatgg gggatacttt ccaaatgttt acagcattaa
     5101 aacagattgg tgtagataca gaaatgtatt tgttccacgg agatacacat ggattgtcta
     5161 gaaatggaag accatctaat agaattgcac gtgcaaatgc tattgtagat tggtttgaaa
     5221 gatatttata ataagggggt tattctatga gtgatatgtt attatggttt agtgtcgtat
     5281 tagttttatt tgctattggg gatttaattg caagtaaaac taaagcaaaa gtatcagctg
     5341 tatttgttac actactttta tttttgattc tatttgtcac aaaagcaata ccagctgata
     5401 ttattgaaaa agcaggtatg accgcagcag ctagttggag tgttccaatg attatgttta
     5461 gcatgggaac aatgttgaat gttaaacaat ttatagacga atggagaaca gtattgacag
     5521 catggcttgg aattgtagca gttatcgttt gtgtctcatt atgtattcct ttgtttggaa
     5581 aatcaacagt attgacaagc ataccagtta taaatggagc tttacctgct actactatta
     5641 tgacacaagc agcactagaa aaaggactga cacttgcagc agctacagca acagttgtat
     5701 ttgcaattca gaagtttatt ggtacaccaa tagcttcaag agcagcacta caagaggcaa
     5761 atcgtctttt agtagaatac catgaagcaa aatcaaaagg cattgattta gcaaatgttg
     5821 atactgataa gaaagaagca gagaatacag gcacaaaagt aaaacaagct ttctgtgaaa
     5881 aatacgataa atattattct acaaatgttt gtatattttt tatagcacta ttctcttatc
     5941 tagggtatga actttcagag attattcatg taaattattc tattgtatgt cttgttgttg
     6001 gtgttcttgt aacacgtatt ggtattgtgc caaaagacat tttggaaaaa ggaaaaatca
     6061 agggatttat caacatggtt gtgtttgcag ctgttattcc ttctttagca aaagtttctt
     6121 tgacagattt aattagtctt tttgtaccaa ttgtaggtat gtttgcagct tctatcattg
     6181 gtatattcct tatgatgaag gtgttaccag gttggaaaat tatcggttct aaacctcttg
     6241 cttttggagt agggttttgt cagatgttgg gattcccaac tacatattta atttcaaatg
     6301 aagtgtgtaa tgctgttgga gaaacggaag aagaaagagc atatttgatg tctaaaatta
     6361 tgcctaagct ggtagttggt ggcatggctt gtatgatttc tattgtagta gctggtctta
     6421 tggtaccaat gttatagata gatttcatag ttaaaaaagt gctattaatt tataggagaa
     6481 gctaaaaagc agtattaatt tataaataaa gctagaaagt aaaattaatt cataaacgaa
     6541 ttttatatta gttaatttgt tataagaata ttttcttttt agcattataa tcttgatttt
     6601 atcaaggaac agatttttta tttgctattc taatagttac agtttatttt tattttctaa
     6661 aatacaactt taacttgatt tatgtttaaa tagggtattt aataatattt ttggaaaatc
     6721 tttagaaaga attaaaaaga gaagaaaaaa tggagtgcct caaagtgaat ttcttagttc
     6781 ataaggcact cttttttgta tgaaatcgaa tatattttaa tcattttata agagatggag
     6841 aaaatttttc tcaaagacaa tgtttaacat tagacttgta tcaagaaggt gaaaatacaa
     6901 ttcaatagtg agaaagagat ttatatctag tttgagacaa gtctttttaa ttattaaagt
     6961 tattttttat cttatttaaa aaagagcatc agtattttat aaattagtat tttttataag
     7021 ta
//
LOCUS       DAEMAE010000192         7014 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEMAE010000192 DAEMAE010000000
VERSION     DAEMAE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.194"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            238..1155
                     /gene="pstC"
                     /locus_tag="KPH93_003015"
     CDS             238..1155
                     /gene="pstC"
                     /locus_tag="KPH93_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG1689789.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWIPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTVITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1157..1981
                     /gene="pstA"
                     /locus_tag="KPH93_003016"
     CDS             1157..1981
                     /gene="pstA"
                     /locus_tag="KPH93_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG1689790.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2000..2764
                     /gene="pstB"
                     /locus_tag="KPH93_003017"
     CDS             2000..2764
                     /gene="pstB"
                     /locus_tag="KPH93_003017"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG1689791.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            2792..3445
                     /gene="phoU"
                     /locus_tag="KPH93_003018"
     CDS             2792..3445
                     /gene="phoU"
                     /locus_tag="KPH93_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG1689792.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            3604..4473
                     /locus_tag="KPH93_003019"
     CDS             3604..4473
                     /locus_tag="KPH93_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG1689793.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            4560..5996
                     /locus_tag="KPH93_003020"
     CDS             4560..5996
                     /locus_tag="KPH93_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG1689794.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKNVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESDRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6044..6871
                     /locus_tag="KPH93_003021"
     CDS             6044..6871
                     /locus_tag="KPH93_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1689795.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         2798 a          720 c         1340 g         2156 t
ORIGIN      
        1 ttttaatata gagaaacaac agtatattgt aaagtaaatg tcaaatatag ttttaaaagt
       61 aaaattaatg ttaaaaaagt aaatataatt ttacacttgc ttaacttaat cctaatatag
      121 atttaactta aaaaaagtag aataaaagta caggaaattt gatgaagacg aattataaaa
      181 aattaaattt tgaatacaga acgaaaatcg ataagtatat tttaatggga gggtattatg
      241 atagtaaaat ctagattagt aggtttagaa aaaaatgaca ataatggcaa taagacaaaa
      301 tacgtaattg aaaaagtagc aaaaaatatt tttctactta gtgctttagt agcagttgca
      361 agtttacttt tgataatagg attcgtattt tataaaggat taagaccatt tatatttgaa
      421 ggatattcat ttatagactt tttaactggt gcagattgga ttccttctgc taataaattt
      481 ggaatatcat ctatgatagt agcatcaata tttgcaacac ttggagcact tgtgatagga
      541 gtacctgttg gtatattaac ctctgtattt atagcagaag tagcacctaa aagattagca
      601 aaaataatgt caccagcagt tgaactttta gcaggtatac cttcagtgtt atatggtgta
      661 tttggtcttg cagtgatagt tccaactata caaagtatat ttaatcttcc aaaaggtcaa
      721 agcttgttag caatagtaat agtactagca gtaatgatgt taccgacaat aatatctgta
      781 tcagaaacag caataagagc agtaccaaaa gcatacaaag aaggttcatt agcacttggt
      841 gcatcaaaaa tagaaacaat attcaaagtt gtattacctg ctgcgaaatc tggagtatta
      901 gccgcagtag tgttaggagt aggaagagct ttaggtgaaa caatggcagt aatacttgtt
      961 gcaggcaact caccagttat gccaactgta ataacagata gtgtaagacc acttacaact
     1021 aatatagcac ttgaaatggg atacgcgttt ggtacacatc aagaaatgct atttgcaact
     1081 ggagtagtat tgtttacatt tatattaatt ttaaacttag ttttaaataa actttcaaat
     1141 aaggcggtaa attaagatga gaaagttcaa agagaatcta ctgaagtcat tagtatatct
     1201 atcagcacta tttacaatag tatcattagt agttatagtg ggatttatat ttataaaggg
     1261 aattggaaat atgaatttta atttcttatt tagcaattat tcagcatcag gtgatggtgg
     1321 tatacttcca atgataatta caacattata tacagtagta gtttctatag ttgtagcaac
     1381 tcctatagga atattggctg ctatatattt acaagaatat gctaaaaaag gaaaagcagt
     1441 tacagctata agatttgcaa ctgaaagttt atcagggata ccatctatag tatatggatt
     1501 atttggagga attttctttg tagttacatt aaaaatggga tattccatag ttgcaggttc
     1561 acttacagtt gcgataataa tattaccagt aataattcgt acaacagaag aagctttaaa
     1621 aacagttcct caagcttata gagaatcatc attggcatta ggagctacaa aatttcaaac
     1681 tttatataag gtagtacttc caagtgcaat accaggaatt ttatctggag taatcttatc
     1741 agttggtcgt ataattggag aatcagctgc aatattactt acagcaggta ctgtagccaa
     1801 gatgccaggt ggaatatttg atagtgcaag aacattaact gtacactcat acttactgac
     1861 taaagagagt ggagatatag caacagcagc cagtatagga attgttttaa tagtaattgt
     1921 attagcacta aacatgttag caagatttgt tgctaagaaa ttaaataaag ctaattatta
     1981 aatttaagag gtgtgaataa tggaattaat cgataagata aaaatgagtg taaaagattt
     2041 ggatttattt tatggtgata agcaagcatt aaagaaaata aatatggata taaaagagaa
     2101 taaggttact gctttaatag gtccatctgg atgcggaaaa tcaactttta taagaacgtt
     2161 aaatagaatg aatgacctaa tcgaagatgt aacaattaaa ggaaacattt ctgttgatgg
     2221 agaggacata tatacctcag atgatgttat aaatcttcgc actaaagtag gaatggtatt
     2281 tcaaaagcca aatccatttc ctatgagtat atatgataat gtggcatatg gtcctcgtac
     2341 acatggacta agagataaga agcaattaga taaaattgta gaagaaagct taaaaggtgc
     2401 agctatttgg gatgaggtta aagacaggct taaatcatct gcattaggac tttctggtgg
     2461 gcaacaacag cgtatatgta tagcaagagc aattgctatg agaccagaag ttattcttat
     2521 ggatgaacct acatctgctt tagacccaat ttcaacatta aaagtagagg aacttataga
     2581 ggatttaaaa aaagactaca caatagtcat agttacacat aatatgcaac aagctgctcg
     2641 tatatcagat gaaacagctt ttttcttaaa tggagaggtt atagaattta gcgatacaaa
     2701 aactatgttt accactcctg tagataaaag aacagaagac tatataacag gtagatttgg
     2761 ttaaaatata taactataaa ggagttagac aatggtcaat aatagtttag atataagtat
     2821 tgatacttta aaagaacaaa cagtacttat gatggaaaaa tgtgaagaag caatagaaaa
     2881 gtctgtgact tgtatgatag agaaggattt agagggtgca agaaaagtag tagaagatga
     2941 tgaggaaata aataaattaa gagaagaaat aagagataag agtatagaac taatagtatt
     3001 aaagcaacca atggcaagag acttaagatt tatttattca ttaggtaata tatctataga
     3061 gttggaaaga ataggagatt atgcagcaaa tatagctaga gaaatgttga agataggtgg
     3121 agaggcttat ataaaagagc tgatagatat tcctaagatg gcaaaagaat gtaagtctat
     3181 gatgtctcag actagagaag ctttaatctt atttgatgca agtatagctt atgatgttgg
     3241 aactaaagat gatattgtag atggattata tgaacaagta caaattgatt gtttaaaaat
     3301 aatgcatgaa aactcaaata ctatagacca aggggtaaaa cttctactta taggaagata
     3361 tcttgaaaga attggagacc atataacaaa tgtatgtgag aagataattt ttgcaattga
     3421 gggtaaaatg gttgagatag gataaaaaaa ctgctttaaa gcagtttttt tattgtctaa
     3481 attaagaaag aattgataaa aaaatatgat taatgggtaa atttaaaaaa ttgtgatata
     3541 ctaggaacat attaaatatg aggatgcgta ttttatataa ggtgcttttt aggaggtgga
     3601 actttgaaac ttcaaaagat gggtatgagg actataaaaa caggaattag tgtcacttta
     3661 tgtatgattc ttggtgacta tttagtagaa aatatgtttt attctctagt agcatgtata
     3721 atttctgttc aagatacggt aaaaggttct ttaaagacag gaatcaatag agtggaaggt
     3781 actattttag gtggtataat aggattcata tttgccatta ttaaagctgg agaccctttt
     3841 ttatgtggtc taggagtaat gtgctcaata tatttatgca atctgtttaa aataaagtca
     3901 gtagtagttg cctgtgtaac ttttctggct atacatctag gtgtaggaaa tagtgaccct
     3961 gtgtattatt ctttacatag ggtgttagat acatctgtgg gtgttttgtt tggagtactt
     4021 gttaattatt atgttgctag accaaattat ctaaatagtg ttatagatga atttaaaaag
     4081 atagaagaac tttcaatgtt gcttattgaa gataaaattc ttagaaaaga aaatttaaat
     4141 gtagagaaat ttgaagaaga agtagaaaaa ttagaagaaa tatattcaaa acttattgat
     4201 gagttggatt atagtttgag tgaaactaat atagaaaaaa ttgagaatgc cttaaggata
     4261 tgtagagaaa tacattttca tatgcagtct atagagttac ttgaaaataa actttatttg
     4321 aaagagcata catataaata ccttaagaaa acatataatg tggataagct agactggaat
     4381 atagatgaag aaaaaagccc tgtgttcaac tatcatctaa tgaaaataat gattgaaata
     4441 gagttgttga gaaagctaaa taaatcatat taataatagt agttaaataa taaataaaaa
     4501 atagtatctt tgtaaatatt aaacatatat aagtttaatt tatggaggta ctatttttta
     4561 tgaatactag aaatgacaat tctaaaatac tagaaatata taaaatgaag ctttttggcg
     4621 aacttgtaaa agcagtgtgg aatgatgata tagaggatat tgaaaactta cctaataaaa
     4681 ttttaaatga agatttaaag gataatttaa ataattattt tagcggaaaa tttgataaag
     4741 atactatggt aaatttaatt agggtaacta tgggattgga tgtgacagaa gatacagata
     4801 aaaatttaaa agagtcacta catgaagctg taaatttaga tgaagtcaaa agaccaataa
     4861 tatctgtaat atcagatgct tgcaaatatt gtgatgaaaa taataaggaa gatgagtcat
     4921 gtaagataag aagtaagcat attaattgta atgaggaaga tgtatgtagt gcttgtggtg
     4981 attgtgtatc aagatgtaag ttgggtgcaa tttcagataa aatagaattt ataccaatga
     5041 taaaattgtt aaaagatata aagagccctg tatatgcaat agttgcacca gcttttgtag
     5101 gacagtttgg gaaaaatgta agccctggta aattaagaac tgcattaaaa aagataggat
     5161 ttgaagatat gatagaagtt gctttggcag cagatatgct tactgcaaaa gaagcatatg
     5221 attattatga gcatatgaaa aaggacgaag aaggatgctt tataactagt tgttgctgtc
     5281 ctatttgggt tagtttgata caaaatagtt ttcctcagat attggagaat gtatcgccat
     5341 ccgtttctcc tatgattgct tgtgggagag ctgtaaaaat tttaaatcca gatgctaaag
     5401 ttgtattcat aggtccttgt atggcaaaaa agaaagaagc gatgttagaa gatataaaag
     5461 atgcagttga ttttgtgctt acatttaaag agttgcaaga aatatttgat gcattagagt
     5521 taaatccagc aataatggat gagagtgata gagtagaggc gtccctttca ggtagagttt
     5581 atgctagaac aggaggtgta agtaaagcag ttaagttatc agttagaaga attgacaaaa
     5641 atataaaatt tgaagaaaag gcatttgaag gaacaaaaga gtgcatggac ggtctaaaga
     5701 aagttctgaa taaagaaata gaagctacat ttatagaagg aatggggtgt gtaggaggct
     5761 gtgtaggagg tcctaagcgt attttaagtg tggaagaagg tactaagaat gttgatgaat
     5821 atagtgaaag tacgaatatg gaaactccat ttgataattt aaatgtatta caatttttaa
     5881 cttctttggg aatcaaaaga gtagaatctt taggagagaa agaagaagaa aaggttaaaa
     5941 aaatatttag tagagatata aaaagtaata agaaagagaa taaatataaa aaataacttt
     6001 ttaagtctta aacttatgct tttataaaag ggagatatgg gggatgaaaa ttaaagataa
     6061 taaggctgtt tttcatgtat ttataaggat atttcttata gctgttttaa ttttaactgg
     6121 aacattattg gaggaaaaaa ttaataatgt tccagttgaa aatataagtt caaaaggtga
     6181 aaatacactg gaactgcttt ctcaaagtaa tactttagga gaaatcatat acaaagcatc
     6241 tcaagaagaa acaaaaggtc caggggctac ttctgaaaaa gtagcataca taacaataga
     6301 tgatggtcca tcaaaatata caaatgcact acttgatatt ttaaaagaaa atgatgtcaa
     6361 agcgacattt ttcatgctta atcaaaatat gaaaaaccac aaggaagaag taaggagagt
     6421 cttggaagag gaaaactcaa taggttttca tagtgtaagt catgatatac ataagttata
     6481 taaaagccca gaagtaacag ttggggaatt taatacatgc aataatactc tacaagaaat
     6541 aactggtcag agttcaaaat taataagatt accatacgga agtaaaccat atatgccaga
     6601 aggttcatat aataaactta tggcaaatga ttatttaatt tgggactgga atttggatac
     6661 acaggattgg aagtcttcta caagtcagat agtaagtaat atattatatt atggaagaaa
     6721 aagagatgaa ttagtagtat taattcatga gaaagagcaa agtttaaatg ctttaaataa
     6781 tattataaga atcttaaaag agagaggata tacaatattg ccaattacag aaaatataac
     6841 acctaaaaat ttctggcaag aaaacttata ataaatttaa atatggtaaa agataaagta
     6901 aattatttat aaaataaaac atataatata aaaaatacga ataagagata tactataagg
     6961 ataatataat ataaaattaa tgagaaagca ttaaaattaa aatagaataa tctt
//
LOCUS       DAEMAE010000193         6990 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEMAE010000193 DAEMAE010000000
VERSION     DAEMAE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.195"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(124..1515)
                     /locus_tag="KPH93_003022"
     CDS             complement(124..1515)
                     /locus_tag="KPH93_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG1689796.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            complement(1971..3113)
                     /locus_tag="KPH93_003023"
     CDS             complement(1971..3113)
                     /locus_tag="KPH93_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1689797.1"
                     /translation="MSEEKNPKENILDESKNSDNVNSKKDDVSDEKNTDNKVKKREVK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKNNYLQVRASHILIKTVDDKGKQVSNSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSNLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            complement(3224..4267)
                     /locus_tag="KPH93_003024"
     CDS             complement(3224..4267)
                     /locus_tag="KPH93_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689798.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKSIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTLKLDDKEAIINDKDIELCKKEVQ
                     VESEKIGLNKFEELDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWFFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKN
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDVLEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            complement(4789..5346)
                     /locus_tag="KPH93_003025"
     CDS             complement(4789..5346)
                     /locus_tag="KPH93_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689799.1"
                     /translation="MNKLLRPITILLYIVYILSAVFFVANKEYGSLGLVAISLAGTFI
                     LSFINKQTERLLSKELYIILALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKLFLVIFLFMFSMGVASLWEIGEFLMDTFIGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSSI"
     gene            complement(5374..6900)
                     /locus_tag="KPH93_003026"
     CDS             complement(5374..6900)
                     /locus_tag="KPH93_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1689800.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTNCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMAAVLENKLADGMFISIDDPSITFRQY
                     NDGNENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKKVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDHIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
BASE COUNT         2295 a         1244 c          614 g         2837 t
ORIGIN      
        1 tataatatct atttgtgtaa agcttttttt cataatcaaa gaggaaatta catttaattt
       61 tatatttaaa tgtaacttcc tttatttaaa gtaattcatc tatttacaat aattcgtcat
      121 gaattatttt tcttttgtta gtaaatccat agccattaat cttgttaata catttcttat
      181 tcccaataat attaaaccaa atattattaa aactgttgct attcctccaa atatctgtaa
      241 aaaaccaagt gtggccgtaa aaccagcaat ctttcctgat aataaacttg caaaaaaact
      301 tgttagatac catgctccca tagataacgt ggcatactta gctggagcta atttattgaa
      361 cattgcaagt cctataggag ataagaataa ttcacctact gttgtaaata tataagtcaa
      421 tactataaac aatatactag cctttaccat tccatctgga ccattagcta atgtagcaaa
      481 taccataact ataaacccaa cacccattat tatcattcct aatcccattt taactggaat
      541 agagaaatca cctcttttgg tatgagataa tttaacccat agggtagcta atactggaca
      601 taaagccaca cataatatag catttagtga tgtaagccat gctactggca tttcaaatcc
      661 aaatagagtt ctatttacat tatcccttac ataaatcata aaagaacttt gtgtttgata
      721 gtatgctgtc cagaataaga ccacaaataa aaataaaata accattgcta ttatcctatt
      781 tttctccaac ttagtaagtg gtccatactc aattttttca cctttattgc ctttagtcac
      841 aggatacttt ccgacttcat ttaataattt tggtgcaaga agtgtaaata taatcatacc
      901 taacatcatg cctataccac aaattaaaaa cgccatctta tatccataag atactatatc
      961 tccacttgct cctcttactg caaatacatt atcagaaagc aatccacata ttattggagc
     1021 tataaatgcc cctacattta ctgccatata gaataaactg tatgctgcat cttttcttga
     1081 cacatcagtt ttaggatata tatccccaac tatatttgag caacttgatt taaaaaaacc
     1141 atttcctaaa ataattattg atagacctac ccaaaccatg gcaatagatg tagatgaagc
     1201 aaatagtacg aaatctccta ttgctattaa aagtgcacct atagaaatac atttttgttg
     1261 acctaaaact ttatctgcta gccagccacc gactaatggt gtaagatata taagacctgt
     1321 aaataaacca aacatagatg ttgcttcttg agtagaaaga cctaaaccac cttgagctac
     1381 acttgctgta aaaaataata ccaataaagc tgttaaacca taataactaa atctttccca
     1441 taaaattgtc atgcaaataa gccaaaaact cttcggatga ccactttttt tagatacgct
     1501 ttctgtactt gacataaaaa aaacgccccc ttttgtttta tagttaaatt taatattaat
     1561 attgctttaa ctattttagc acatacttat aatatcatct tttctatgtt tactcaatag
     1621 attatcgcct gtattttcat ttttttatta ttatttttaa aattatgaaa aatattattt
     1681 ttttagtatc ttcagtagaa aatcttaata tatatatatt taaacaacaa cttaaatctt
     1741 caattctttt tttgtttatt ttttcatttt tataaatttt catttaaaaa attcaaatta
     1801 aaaatatttc tttttaatat ttttttgcat gttatttctt tatcacttca tatatataaa
     1861 tagattttat ttcaaaataa taaaagctat tttagactct ctatattgag agtaaaaata
     1921 gcttttatcc atacagttaa tttttctttt ttttaataaa ttgactttat ctattcttta
     1981 gctttcattt gatatttatc tgtaatttta acattatatt tttcttttaa attttgtata
     2041 agtaggtttt gtttttctga tgttaaattg cttactaaat ctgattttac ctcttcaaat
     2101 ggctgatatt tttcatcagt tttcttaatt atatgatatc cataatcact ttctacaagt
     2161 ttgttagata cctctccctt cttcaatgca aatgctgctt tttcaaaaga ttccaccatc
     2221 tgacctttac caaagaaacc taaatctccg cctgattctg cactactatc ttcagagtat
     2281 tttttagcta gagtagcaaa atcttcccca gcttgggctt ttttaagtat ttcttcagct
     2341 tctttcttaa gttcagcttt tttactatta ctaacttgtt tacctttatc atcaacagtt
     2401 tttattaaaa tatgtgaagc tctaacttgt aaataattgt tcttgttttt attgtaatag
     2461 ttctctgctt ctttatcagt aacttttgat ttttcattta agtattttga tgctactagt
     2521 tcctttttca tatcatcttt tatatatttt tcagtcaaat caaactgttt taaaaactct
     2581 tctttagtca ttcccattgc tgattctata ctactcatta aattattata ctgagcatct
     2641 agttggtcat tagttatact tattttctct tttgctgctt cagaggataa tatatcagat
     2701 gtaatcatag tttcaatcat atttgattca tatgaacgta tttcatcatc agtcatagta
     2761 gtttttgcat ttaaataaaa taatggctcc attctctttc ttaacatttc acctgttatt
     2821 tttttatccc caatagtagc tactacctca ctgctattat agtgttttga agtagcaggc
     2881 aattttcttc cagcttcttt gccaaaaaca tagcctccac ctaaacatat agctcctact
     2941 gcaacaattg ctacaatagc tttaagtgtt ttgttgttta gctttacttc ccttttttta
     3001 actttgttat cagtattttt ttcatctgac acatcatctt ttttagaatt tacattatct
     3061 gaatttttag attcatctaa tatattttct tttggatttt tttcttcaga cattaatacc
     3121 ctccttaatt ttaattgtgc aatacttcac atttattatt ttacattaaa ataatattaa
     3181 atgtgtgaat cagaaatgaa actcttatga atttaaaatg aaattaaata cttctcaaac
     3241 tataccttat aaagttcctt cctacttttg tatctccata ttttaacatt tgtataactt
     3301 cattcttttc cttttgcaaa taaatggcac attcttctaa agacttaaat atttctaaaa
     3361 cgtcaccttt tttatttact aattcaactt ttttctttcc acctcttttg attatagact
     3421 tatatttttt aaaatattca tcatattctt catcaataca ttcaaatcta taattcattt
     3481 ttaatgcttc aattttatca tttgtcaaaa tttcatttct tttttcatta gaaagtctta
     3541 tactaaatat tttattgttt agaatattaa atatctcaga tggagatttg ctattatcga
     3601 gataactcca ttcatttttg cagtattcat ctatgtttaa tacttcactt aaataattta
     3661 ttgcatctcc aaagtaatcc atatatccat attttaagtt ttctcttata taccccaatg
     3721 gaactttgag ttttttacta cactgtatat gagtgtcaaa aaaccactct tctttagtat
     3781 acaagtttat agcttttata ctttttcctc tttttatttt aatttctttt atgtactcta
     3841 aatcttcttc tgtcaaacat tcacttactt ctttttcttc aataacattt ttttctagat
     3901 tataattttc attttggtca agttcttcaa acttatttaa acctattttt tcactttcaa
     3961 cttgaacttc ttttttacat aattctatgt ctttatcatt aataattgct tctttatcat
     4021 ctaattttaa ggtattctca ctaattaacc aatcttcttc tattattttt tcttctattt
     4081 cattatcatc atttactact tctactttag ttaatatttc attatcaata ttaaccacaa
     4141 ctccttcttc atctatcgac ttttcttcag ataatatttt ttcgtttttt aatattactt
     4201 caccttcttc ttttactgac tcactttttt gtcttttttt cacaatacca aaccactttt
     4261 ttaacactca atgttcctcc taatacttta ttactattct atactatcat ataaaatatt
     4321 actgttctta taaatcactt aattttcata tgtttttgat atgatactcc ccatgtttaa
     4381 agaaaagata ttcaagtgaa aaagatgacc tatttctagt agcccaacat gacatcaaat
     4441 ttaagatatg caattactgt tcctaaacta atccatacat caatttagac tagtagattt
     4501 ttactttcta caccattatc aaaaaactta ctattatact taggatacaa tataacattt
     4561 acataaatag actatattat catttgattt gtatttattg cccataatgg tattatatat
     4621 taattattta cataatccaa caatttctat ctatcaccgt atatctcata tgtaaaaata
     4681 tattcaataa taattataaa taacaaaaat ggatttaatt aatcatatag accttgaaac
     4741 tcaacatgat taattaaatc cattataact aatataaaaa atttaaaact atatactaga
     4801 tttttttact ataaaaggaa tagttataat tgtacctatt aaagcatcta ccatatcaat
     4861 catagtatct tctaatcctc cagcctgtgt agtagtccca ataaaagtat ccattaaaaa
     4921 ttcacctatt tcccataaac tcgctactcc catactaaac ataaatagaa atataactaa
     4981 aaacaattta ctcattttac ttatctctct ttgtgtataa aagtatttaa ttatagaaaa
     5041 agccacaata caagatatta tacctgacca taaatgcaaa aaatcatcat aataatttat
     5101 atcataaaaa ttaaaacaag tacctaataa tgatgaaaac attataaata aagcaagtat
     5161 tatatataat tctttactca ataatctttc agtttgctta tttataaaac tcaaaatgaa
     5221 agtaccagct aaactaattg ctactagacc tagacttcca tattctttat ttgctacaaa
     5281 aaatacagca gataaaatat aaactatata caataatata gttattggcc ttaaaagttt
     5341 attcatatac actttacctt cctaaaaata attttataat aacttaggat taacatcatt
     5401 atcaccatgt ccataaagtt ttaatggctt aagagctggt ccttcaagta tatttccctt
     5461 atagtcaaat ctagaaccat gacatggaca atcccatgtt ttttcttcac tattaaaatt
     5521 aagttcacat cctaaatgag tacatgtagt gtctacaata taaaaatccc cattatcatc
     5581 cttatataca ccatatcttt ttccatcaat attaacaatc tttccttctc cattattttt
     5641 aggcatatct ccactaccta tttttaattt tccacttaca taatttattg ctacgtttgc
     5701 attttccttt ataaaatctt ttgaaaagta gctacctaat ctggatggat taaaagtatc
     5761 tttataatca gaattattat ttattatcaa atcttttatt attattccag cagcagttcc
     5821 attagtaatt ccccatttgg agaaccctgt agctacataa atatggtctt ctcttttgtt
     5881 gatataacca atataaggaa cattgtcaaa actcatatta tcttgagcag accatttccc
     5941 cttaaaatct tttaccttaa agactttttt cccataatgc tctaaatcat caaatatttc
     6001 tttctctgtt cctccctgac cgactttatg gtctcctcct ccaaaaatca gtaaattttc
     6061 atttccatca ttatattgtc taaaagttat tgatgggtca tctatactta taaacatacc
     6121 atctgctaat ttattttcca aaactgcagc cattaaataa gcacgctctg catattcttt
     6181 tgcaaaataa aaattcaatc catcatacca tggactatgg gatgaaatta taacattctc
     6241 agcttctatt atattatcct ctcttgtttt aaccctacat attttgcctt tttcaaggtc
     6301 tactactggt gtattttcat atatttttag ccctaaattt actgctgctt ttgccaatcc
     6361 atcaatatac ttctttggat taaattgacc ttgattagta aaactaatag ccccctttat
     6421 atctaatgga atattttcta tagtttcatg atactcacaa tttattccta tttttttaca
     6481 tgtacgatat tcatctttaa tattctttat agtatcctcc ttttctgtaa atacaaaaga
     6541 agtttccctt ttaaaatcac attttatatt gttttcttct attatttgtt caactaaatt
     6601 taaagcttca ttatttgcat tataatatga ttttgcttta tctaatccat attttttctc
     6661 tatcttagag taaaatatgt catgctgaca agttacctta ccagtattcc ttccagaagt
     6721 tccatatcct attttatcag cctcaacaag cactactttt ttaccttctt ttgctaataa
     6781 atatgcagtt gtcaatccag ttattccacc acctactata agacaatttg tttttatatt
     6841 ttcacctaat ttgttatatt cttttggact tgagtttaaa aaccaatatg attcgttcat
     6901 ttaaaatact cctttacata ttaaatagta atagttttcc catcatggag tttatttatt
     6961 ataaatagta taaaaaataa gcttaataaa
//
LOCUS       DAEMAE010000194         6987 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEMAE010000194 DAEMAE010000000
VERSION     DAEMAE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.196"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    159..369
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            595..1410
                     /locus_tag="KPH93_003027"
     CDS             595..1410
                     /locus_tag="KPH93_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1689801.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1438..2115
                     /locus_tag="KPH93_003028"
     CDS             1438..2115
                     /locus_tag="KPH93_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG1689802.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2174..2896
                     /locus_tag="KPH93_003029"
     CDS             2174..2896
                     /locus_tag="KPH93_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG1689803.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            3541..4692
                     /locus_tag="KPH93_003030"
     CDS             3541..4692
                     /locus_tag="KPH93_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG1689804.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFKPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            4738..5913
                     /gene="thiI"
                     /locus_tag="KPH93_003031"
     CDS             4738..5913
                     /gene="thiI"
                     /locus_tag="KPH93_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG1689805.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            6069..6872
                     /locus_tag="KPH93_003032"
     CDS             6069..6872
                     /locus_tag="KPH93_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1689806.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEEQSKDKNEVIKEVLQNKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSAKSVKTEIHSKEGWGPLN"
BASE COUNT         2792 a          684 c         1339 g         2172 t
ORIGIN      
        1 taaaaatatg aaatatacta tttataatta cacaaataat atcaataaat ataaacagta
       61 gtatttagat aatttacaag taaaaattat taaaaaaata aaaaacttat tgacaaaaat
      121 attataagga agtacaattg tattaacaat ttaaggaaac aaatccaatg atagagaaag
      181 tagcatatat ggatttacag cgagttaggg attggtgtaa gcctagcaat gaagtctatg
      241 tgaagagagc tctggaggag attatcagaa actttagtat gataattggt ttacccgcct
      301 taagggattg agaggataag tacttactta tcaaaaagag tggtaacgcg gatataattc
      361 gtctcttagc tgtaaagcta agggactttt ttgatttaaa aataaaaata tccatacagc
      421 ttaaaagcta acagtcacaa attttgaaag taatataagt atttatcaat aagagtggta
      481 acgcggtaaa gtcgtctctt agtcattaaa gactgagaga ctttttttgt aactcgaaac
      541 atttaagtat taaaatttga gggaaattat aattaaaaga gaggggtagt taaaatgaaa
      601 gtatttaaaa aattattaag tttaggatta gtattaggat tgacattatc gctagtagga
      661 tgtagtggtg gaggagaaaa aacaaagtta gagcaaataa aagacaatgg aaaattggtt
      721 gttggtacta gcgcagaatt tcctccattt gaatttcata aggtagtaga tggaaaagat
      781 tcaataaaag gattcgatat aatgttggct gaagaatttg caaaagagct tggtgtaaag
      841 gttgaaatta aggatatgtc atttgatgga ttaataggtg ctttaaatgc agaccaagtt
      901 gatattgttc ttgcaggtat gtctccaaca ccagagagag aaaagagtgt tgatttctca
      961 gaattatatt atttaagtag aaatgcagtt atagttaaag atgcagatat agacaaagta
     1021 aaaactgaag atgatttaaa gaaacttaga gttggagttc aagcaggaag tatccaagaa
     1081 gaatatgtag ttaacacttt aaaaatgaca actacaaaat ctttaaaagc aatacctgat
     1141 ttaattacag aattaaaaaa tggaaacata gatgcagttg ttactaacga agcagtttct
     1201 ttaataaatg ttaaaaaata tgatggaata aaaatggcaa atactgaagt tgggaaagat
     1261 gtaacagaag gtatggcagc agcaatcaaa aaatctgaca ataacaaaga ttttatagaa
     1321 ttattaaaca aaaagataaa agaattacaa gatggcaaga agatagaaga atttttaaat
     1381 gaagcatcta ctgaagctgc ttcaaattag taagaaagtt atgtgggaga tgaaattatg
     1441 agtttagatt ttagtttttt aagtagattt gggacttctt ttttggaagg aacaggtgtg
     1501 acagtatcaa tttctcttgt ggcattatgc tttggattta taataggtat aattatctgt
     1561 atggcaaaaa tatcaaagag caaagtatta agagcaatat catcaatcta tattgaggtt
     1621 ttaagaggga caccattatt agtacagata tatatagtat ggtttggttt acctcaatta
     1681 ggaataagat tccctatgtt gtttggtata ccaagtgaat ttatagctag tgcatttgca
     1741 ttatcagtaa actctggtgc atacgtggca gagatactta gatcaggaat acaatcagtt
     1801 gataatgggc aaatggaagc aagtagatca ttaggattaa attattggtc aactatgaga
     1861 tatataataa tacctcaagc tataaaaaat atattacctt cattagcaaa tgagtttatt
     1921 acacttgtaa aagaatcttc aataatatct gtaataggag ttgttgagat aatgcgtact
     1981 gctgatatag taaaaaatgc agcatttaga gcattagagc cattaatagt agcagcagca
     2041 atttactttg ttataacatt tactctatca agattagttg gattattaga aaagaaacta
     2101 tctgtgtcta actagatgag agcatgttta gcatttaggg aaaacataga atagtaaaaa
     2161 ggaaagtgat aatatgatta caataaaaaa tttaagtaaa tcttttggag atttaaatgt
     2221 attaaaaaat atagacttag aaatcgctaa aggcgaaata atggttatag taggtccaag
     2281 tggttctggt aaaagtacac ttcttagatg tatgaattta ctagaaatac ctacaggtgg
     2341 agaaataata tttgaaggaa aaaatttggt tgataaaaaa actaatattg atgaggttag
     2401 acaaaatata ggtatggtgt ttcagaactt taatttattc ccacataaga ctattttaga
     2461 taatataaca ttagcaccaa ttaaattaaa aaagatgact aaggaagaag cagagaaaaa
     2521 agcagaaatt ttgttgagta gagttggtct tttagataaa aaagattcat atccatcaca
     2581 attatctgga ggacaaaagc aaagaatagc aatagctaga gcacttgcta tggaaccaga
     2641 tatgatgttg tttgatgagc caacatctgc tcttgaccca gaaatggtaa acgaagtttt
     2701 ggatgttata aaagaacttg ctaaagaagg tatgacaatg gcaattgtga ctcatgagat
     2761 gggatttgct aaggaagttg cagatagagt tatttttata gatggaggaa gtatacttga
     2821 agataatact ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa aagcattttt
     2881 agcaaaagtg ctataatata taaaaaggat gttattgata ttagaaattc aatacatcct
     2941 tttttagtga tggttaatat tagttttaaa attaaagtta tattaatatt aactttattt
     3001 tacgattttt tgcagaaaaa aaagagctcg gggtaagctc ttttttccac cgaattaatt
     3061 tccgttacta atccgatttt taataaatat aaaatatatc cttatgaaag ggtattggga
     3121 atagatatat taactaacta gggttaccag ggggataacc ttaatttatt ataacaaaat
     3181 gcgacacaaa aatcaatatt ttttttgaaa taaattataa aagttatttt tataacaagt
     3241 aagcttataa atttaacttt tatagtaaaa ataagtacat gtataatata atatatttat
     3301 tttatataat atattatttt atcatatatt gtattttata atgtattttt gtagggtata
     3361 gtataagtat attatatttt actatgaaaa tttgtaataa agtgaaaaaa ctagcttttc
     3421 atgaaataat attttataat ttaagattgt ctttttaata aagattaaca aaaaaaatac
     3481 tataattagt ggtataatag ttagagttga agattttaaa taaaggtagg tgtaattaag
     3541 ttggaaatat acttagacaa tagcgcaact acaaaacctt atcaagaagt tatagataaa
     3601 atggtttatg cacttaatac ggaatatgga aatccttctt ctgtccatag aaaaggtgta
     3661 gaagttgaaa aagctataaa ggaagtaaga caagatatag ctaaatctct aggagcgaaa
     3721 gaaaaggaga tttattttac atcaggtggt acagagtgta ataatactat aataagaggt
     3781 attactagtt taaataaaaa aagaaaaaat cacataatat ctactaacat agaacatcct
     3841 tctgttttaa atacattaaa agatttagaa gaagatggtt ttgaagtaac ttatttagag
     3901 gtcggaaaag atgggaaaat aaatatagag gatttaaaaa atgcgataaa gtcaactaca
     3961 tgcttagtaa gtatgatgca tgttaacaat gaaataggga ctatacagcc aataggtgaa
     4021 gttggaaagt atttgaaagg tttaaaagaa aagatttatt tccatgttga tgctattcag
     4081 tcatatggaa aaattaactt taaaccatct aaatacaata tagattttat gagcgtaagt
     4141 gcacataaat tccatggtcc aaagggaatt ggatttatgt atataaaaga aaataatcgc
     4201 cttaagccta tgttaacagg tggtggtcag gaaattggaa ttaggtcggg aacagaaaat
     4261 gtacctggga tatatggtct tggagaagct gttagaatac taaataaaga tttagatgct
     4321 gtcatttcga aagtaaataa cttaaaaaat atattaaaaa atgaaataat tgataatata
     4381 gaagacataa aaattaattc tccagaagat ggagtatgtc atatattaaa tgtatcattt
     4441 agagggacaa aaggagaagt tttactccat tacttagagc aaaaaggtat ttatgtatca
     4501 actggctctg catgttcctc taaaaagaaa ggtagttacg tattaaatgc tataggtctt
     4561 acaaatgaag aaataaatgg aacaataaga tttagtttat ctgatatgaa tacagaagaa
     4621 gaaatgttag aagctgtgaa agttttgaaa gaatcaattt gtgacttaag gtcaataatg
     4681 aaaagaaaat aaagaatact gtataaatag tgataataaa ttagtttaag aggtgaaatg
     4741 gtgtataata tattaatcgt aaagtatgga gaaattggag taaaaggaaa gaatagatat
     4801 atatttgaga ataggcttat aagaaatata agaaatatgt taaaaccaat tggaagattt
     4861 aatgtatata aagagtatgg aagaatatat gttgatttag aagactatga ttatgaagaa
     4921 gtaattgaag aagttagaaa agtatttggg atagtgggtg tgtgccctgg agtaagagct
     4981 aaaaaagatt atgatacatt aaaagaaata gctttaaaaa tgttagaaga aaaaatagag
     5041 gctggatata aaacttttaa ggtagagtca agaagaggag ataagtcttt cgggttgact
     5101 tctcaagaaa tgagtatgga tataggtgga tacttattat caaaggtagg agataggatt
     5161 aatgttgatg taagaaaccc agaagttaaa ataaagtgcg aatacagaga atttcatact
     5221 atggtctata gtgatactat acctggatat ggtggattgc cacttggaac gaatggtaga
     5281 gctatgtctc ttttatcagg aggtatagat agtccagtag ctacatggat ggttgcaaaa
     5341 agaggtatgg aagtagaggc agttcatttt catagttatc catttacaag tgaaaggtca
     5401 caggaaaaag ttaaggattt agctaaaata ctggcaaaat actgtggtag agtaaggctt
     5461 cataaagtta atatcttaga aatacaaaaa gcaattggcg agaattgtaa tgaagaagaa
     5521 gctactattc tttcaagaag atttatgatg aggatagcac agagactttc tgaaaaaaga
     5581 cattgtgatg ctttaattac aggtgaaagt attgggcagg ttgcatctca aactatacaa
     5641 ggacttactt gtactaatgc cgttgtagac ttaccagtgt ttagaccact tatagcaatg
     5701 gataaatcag atatagttga tatagccaaa aaaataggga cttttgaaac atctatagtg
     5761 cctgaagaag attgttgtag tgtattttct cctagaaagc ccgtcactaa acctagatta
     5821 gagaaaatag aaaaatctga aacagcactt gacattgaaa aattagtaca ggatgctatt
     5881 gacaaaatag aggtagagga tattgagttt tagcatacga gttatttaat tattaaattg
     5941 aatattgagt atattagtag ttctgtaagt atattagtag ttctgttgaa aggatttatg
     6001 tagacaattt attaatatgt agtatttaaa aataagtact ataattttat ttaatgggaa
     6061 tgattattat gaataaaaag ggttttacac taattgaatt gttagtagtt atatctataa
     6121 taggcatttt agttatagta gctgttccag cattgtttag aaatatagag aaaagtaaag
     6181 cagttacatg tctttctaat agagaaaata taaagactca aattgttatt gcaatggcag
     6241 aggaacaaag taaagacaag aatgaagtca taaaagaggt attacaaaac aaagatggta
     6301 agtactttga aacagaacca aagtgtaagt caggtggaat atattcagca acgtttgatg
     6361 atggttatga tggaataact ggaatagaaa gcattgcaaa agtgtatgtt acttgtacaa
     6421 aacatccaga tggtgttgaa atggctaggg atgtacatca aagtatgaaa gatttgattg
     6481 catcatttgc acaagaccct tctataatac caggagcttc aaagggtaat gatgatttta
     6541 gaaaatattt attagacaat aaatataaaa atgggtggcc tacaattcca gatgaattta
     6601 aggcaaaata tggattaagt aaagatacac tatatataca accatatgca tataatccta
     6661 ctaaatctga tgctactgta gttgtatttg caaataataa gactggaggt aattggtata
     6721 cttctctagt ttacgattat gatgaaggta gatggtataa aggtaaaaat ggtatttctg
     6781 ttgcaggtag gtcatgggat gttgacacag atagtgctaa gtctgtaaaa acagagattc
     6841 attctaaaga gggatggggt cctttaaatt aatataacat gttttattaa tctggtattg
     6901 attaaactat ggaaaatagc agtttctata gatgacgaat accagatttt tatttatata
     6961 taattaagat atattaaaag tgtttat
//
LOCUS       DAEMAE010000195         6908 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEMAE010000195 DAEMAE010000000
VERSION     DAEMAE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.197"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..433
                     /locus_tag="KPH93_003033"
     CDS             92..433
                     /locus_tag="KPH93_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG1689807.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYDV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            584..997
                     /locus_tag="KPH93_003034"
     CDS             584..997
                     /locus_tag="KPH93_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG1689808.1"
                     /translation="MLELKNNSGTSERKLKYPCIYKHFKGKYYATMGLSKAIDNIENI
                     CEIYGRENLIQNRNKYKLVVRHTEREEDIYVYRDLHGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1199..1840
                     /locus_tag="KPH93_003035"
     CDS             1199..1840
                     /locus_tag="KPH93_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG1689809.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIIKMNDTSHIKNHVKINNSALE"
     gene            1864..3117
                     /locus_tag="KPH93_003036"
     CDS             1864..3117
                     /locus_tag="KPH93_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1689810.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKFGDMLFAHFGITGPCVLIMSSYINKIIEKEKIE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLGDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3163..3813
                     /locus_tag="KPH93_003037"
     CDS             3163..3813
                     /locus_tag="KPH93_003037"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG1689811.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQTAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPNADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3864..4460
                     /locus_tag="KPH93_003038"
     CDS             3864..4460
                     /locus_tag="KPH93_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG1689812.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNGDHERISQEILEVIKQNYFKYSK"
     gene            4473..5312
                     /locus_tag="KPH93_003039"
     CDS             4473..5312
                     /locus_tag="KPH93_003039"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG1689813.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5559..6713
                     /locus_tag="KPH93_003040"
     CDS             5559..6713
                     /locus_tag="KPH93_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG1689814.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMLLAHSGLNLPIIEKLHSL
                     EEAIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
BASE COUNT         2862 a          648 c         1199 g         2199 t
ORIGIN      
        1 ttaagtaaat cagtcttaca tatatttccg atagattata ttgataaaaa acactaaaat
       61 taaattaata aaagtacttg gagggatgga aatgaaagag gaaaatttta ttaatgttga
      121 atacgaggat aaacgttttt caattaatag taaagaagtt ttgtacatag aaaagcaaga
      181 atatgaagta aagatttata ctgaaagtgg taattatgac gttggagaaa atgctccttg
      241 tataagaaaa ctattgaaag aagaaaattt tttcaaatgc agtaatagtt atattgtaaa
      301 tctagacaag tgtgaatttt atcttagcaa taattgtatt tttgtgaata ataattttat
      361 aagaatttta ggttcagaaa gaaaaaagaa attaaaaaag aaattagaaa aattatatga
      421 ttatgaagat taatattgag aggctgtatt ttatacagcc tctttattta ttcattgtat
      481 tattaataaa ttttaaatta tgataaaata taaaacaagt aacttatttc agaataaaat
      541 actttttaaa agaaagggac gagatatggg agattataat caaatgcttg aattaaagaa
      601 taactcaggt actagtgaaa gaaagttaaa atatccttgt atctataaac atttcaaagg
      661 gaaatattat gctacaatgg gtcttagcaa ggctattgat aatatagaaa acatatgtga
      721 aatatatggt agagaaaatt taatccaaaa cagaaataaa tataaattag ttgttaggca
      781 tactgaaaga gaagaagata tctatgttta tagggattta catggaaact tttatcataa
      841 aaaagaagaa gatactaatg atttagtttt atacaagact ttatatgatg atacaggaat
      901 atttgcaaga cctttggata tgtttttaga aaaagtagat acagataagt atgtcaattc
      961 aattcaagaa tacagatttg aagaagtata taaataatat aagagaaata tgtttataaa
     1021 tttagaaaaa ttctttaaca gtacaataat ttgcagaaat acaaatgaga tttattgtaa
     1081 ataatatgtt agattagatt ataaaataga aacaaagtaa agtattatat gatataatta
     1141 agttttgaga agtgataatt tatatttaat aaaaaatagg catatattag gaggacatat
     1201 gggaaatact ttttatatag taagacatgg acaaacggat tggaacatat tgggaaaaac
     1261 acaaggtcat ggtaattctg accttacgcc tcaaggtata gaacaggcaa aagaactttc
     1321 tgaagatatt ggcaagtatt caattgatta tatttttagt agcgaccttg ggagagcaat
     1381 gcaaacagct caaattttag gagataaact caatattgaa gttcaaaaaa ctgaggcact
     1441 tagagaaatg ggattcggag tttgggaagg ccttttaata aaagaaattc aaaaagatta
     1501 ttcagatata tatgcaacat ggagaaatga gccacactta gtaaatatac cagaagggga
     1561 aactcttaag attataaagg aaagagtaga tgcttttata aaggaactaa atgaaaaata
     1621 tgacaataaa aatataattc ttgttacaca ttcaattaca ttgagggtta tgttgttgtc
     1681 atttttagaa tcaggtatgg aaaatatata tagaattaag caagataata ctgctttaaa
     1741 tatagtagaa tttaaagatt atggacctgt tataataaaa atgaatgata caagtcatat
     1801 aaaaaatcat gtaaagataa ataactctgc actggagtag gaatcaatta taaggagaaa
     1861 tttatgagta aagttatagt agttggtgga ggagcttctg gaatgatggc agctttgtca
     1921 gcttcaaaaa acaataatga agtaatatta gttgagagaa atggagaatt aggaagaaaa
     1981 cttagggcaa ctggtggcgg tagatgcaat tttaccaata atagagagat agaagatttt
     2041 tttgataaag ttgttagtaa taaaaaattt ctctatagta gtttctatac atttacaaat
     2101 aaagacctta tttcgtattt tgaaagtaga aatttggaat ataagattga agaagaaaat
     2161 gaccacaaag tatatactaa aaatgataaa tctatagaag taatagaagt attaaataaa
     2221 gatttactca atcacaatgt aaagataatg tataataaaa aagttataga tattataact
     2281 gaagaaattg ctctaaaaga tgattctaat aaggacaagt caaagtatct aataaaaggg
     2341 attattttag ataatggaga caagatactt ggagacaagg ttattatatc aactggggga
     2401 gtgagttatt caaaaacggg ttcagatggt tcaatgtaca agattcttaa aaagcatgga
     2461 catacattaa acaaactata tcctgcttta gttccactta caatagagga aaaatggatt
     2521 aaagatttac aaggaatatc catgaagaat gtggaaatat cttgtaagat taagaaaagg
     2581 aaaatatcaa aatttggaga catgttattt gcacattttg gtataacagg accttgtgtt
     2641 cttataatgt cttcttatat taataagatt attgaaaagg aaaagataga attaaatata
     2701 gatttcttac caaatttaag tactgatgaa atctctagta taatcagagc atttccaaat
     2761 aaaaatgttc taaataatct taagcaaata ctacctcaaa atttcttaaa agagattttt
     2821 tctctattat ctttaggtga taaaaaggca agtgatttgt ctaaggcaga tgaaattaga
     2881 attattgagt atataaaaaa tatgaagcta acttgtaatg gaactacagg tataaacact
     2941 ggtatggtta cttctggtgg tatttcagtt aaagaaatta attcttctac tatggaatct
     3001 aaattagtta agaacttatt ttttacaggt gaagtaattg atatagatgc agaaactggt
     3061 ggatataatt tacaaatagc attttcaact ggatatcttg cgggtattag tgtataaata
     3121 taatttttta ataattaaat atgaataaat agaggtgtat atatgggtaa tttagttata
     3181 gctgtagatg gtccagcagg agctggaaaa agtacaatag ccaagatagt agcaaaaaag
     3241 ctaaatataa attatataga tacaggtgct atgtacagag cagttactta taagtgttta
     3301 aaaagtggta ttgatgtcaa taatgaaaaa gaagttattc aaacagctga aaattcggat
     3361 atagatttta aagataacaa tatatatttg gacaaagaag ttataaatga agaaataaga
     3421 acaatagaag taagtaataa tgtttctaat gtagcaaaaa taaaagaagt aagacagttg
     3481 atggttgaag ttcaacgaaa aataggaatg aaaagttcag taatactaga tggtagagat
     3541 ataggttcat atgttttccc aaatgcagat tacaaattct ttttagtagc tactccagaa
     3601 gaaagaggaa atagacgcta taaagaactt tgtaataaag gatataatac tactttagaa
     3661 gaagtaattg aggatataat aagacgagat gaaattgatt caaatagaga atttgcacca
     3721 ttagtaaaag ctaatgatgc actagaaata gatactacag gaaaaactat agaagaagtt
     3781 gtagaagaag ttgtatctaa aataaatctt tagtttttta tgcatcataa tgaaaataaa
     3841 tgtagtgttg aagggagata aacgtgaatt tttatagatt tgtaataaat atttttaagg
     3901 gtttttctaa aatatttttt aaatatgaag ttataggtgc agaaaatatt ccagatagag
     3961 gaaatatagt aatagcatca aatcataaat cgaatcttga ccctatattc ttggctgctg
     4021 ccatagaaaa tagagaaata gctgcaattg caaagaaaga attgtttaaa gtgaaaccgt
     4081 tgggatttat attaaaaaaa ttgcatgtta tgcctataaa tagagaaaag ccagatgtat
     4141 ctacaataaa aactatttta aggtcagtta gagatggtta tgtgttagga atatttccag
     4201 agggtactag aataaaagga gattcttttg gtaaggctaa ggcaggtctt tctgtattta
     4261 caataaaaag taaatctaaa gttgttccag tatcaataat atccaaatat aaacttttta
     4321 gtaaagtaat tgtatatatt ggagaaccaa tatcatttga agaacatttt aaggaaaaat
     4381 tgagtaatgg tgaccatgaa aggatatcac aagagatttt agaagtgata aaacaaaatt
     4441 actttaaata ttcaaaataa gggggtaaag ttatgaacat aaaaatagca aaaaatgctg
     4501 gcttttgttt tggagtaaaa agagcaatga aaatggcatg ggatgaagtt gaaaaaaatg
     4561 attctggcat atatgcttta ggtcctctta ttcataacaa gcaagcagta gcaaaatatg
     4621 aagaaaaagg tcttaagact gtgaatgaaa ttgatacaat accaaatcat gaaaatatga
     4681 taataaggtc tcatggagtt cctgaaaata tatataagga agccaaagat aaaaaattaa
     4741 aaattgtgga tactacatgt ccatttgtaa agaaaataca tacagtagta agtgagtatc
     4801 ataataaagg ctatgaaata atagttattg gagatatgaa gcatccagaa gttataggta
     4861 taaatggatg gtgtgaaaat tcagctataa taataaagac tttagaacaa atggaaaata
     4921 tggaatttga taactctaaa aaatattgtc ttgttgcaca gactacaata aatccagagt
     4981 tgtatataag tatagttaat aaactatcag ataaattaga agaaatagta tttaatgata
     5041 caatttgttc agctacaaaa acaagacaag aatcagctaa agagttagct aaagaagttg
     5101 attgtatgat agttatagga ggtaaacaca gttcaaatac tcaaaagtta gtaaaggttt
     5161 gtgaagattt agtaccaact tttgcaatag agactaaaga tgaattggat gtaaatactc
     5221 ttaaaaaata taaaaatttg ggtattacag caggggcatc tacacctaat tggataatag
     5281 aagaagttgt aacattcctt gaaaatttat aatttgatag gtaataaaaa taggtggaca
     5341 ttgttataat atagaactta tcataatatc cacctttgat ttcatattac atgtggtaaa
     5401 tagataacct aatatcaaaa agtgtgtatc ttaagatgaa aaactaatag tataagatgc
     5461 gcacttttta atattagaaa ttcaaaagaa atattgatat tgattatcaa tagaaaatat
     5521 aatataatta ttgcaaaatt agtctaggag gtaaattaat ggaaatgttg atatccgctt
     5581 ctgaagaggc gtttttacat gtaggttcta tgattggatt ttttattttg ttatttggat
     5641 atattaatta taaaactagt ggaaatttta ctaatatcat atcaaaaaac agaaaatttc
     5701 aacctttaat aggtgcttta attggagcta taccaggttg tggcggaagt ttggcaataa
     5761 tgccattgta tataaatgga aaattaagct ttggagcaat aattgcaagt ttaatagcta
     5821 gtatgggaga tgcagcattt gtacttattt catcaaatat taaaatgtat ttttttgtca
     5881 ctatagttag tactattaca ggaataataa ctggacaact agtggattac tttaaactag
     5941 aagaaaaatt agggttaaaa aaccgtagaa aatcagataa atacgatata agtaaaaata
     6001 actctaaaga aaataaagaa caacataatc atgatgaaat aattttagac actctagcta
     6061 aatctcatgg aaatacaaat agacttgcat tcataataac acatggaaag ggatataaaa
     6121 tttatatagg aattatagta atcggtttta tatttatgtt acttgctcac tctggactta
     6181 atcttcctat aattgagaag ttacattcat tggaggaagc aatagcagtg attggaatat
     6241 tattttcaat aatttatatg tggtgtttta aaaaggtatt taaaaattct aatcaaaaag
     6301 aggaagaaaa taaaaagatt tctttacgag aaatgcttat acattctgtt ggagaaatat
     6361 catttgtaat tacatggatt tttattgcat atcttattta cgatttgata atattaattt
     6421 taggtggaga tgaatactta gttaaactag tattatcaac aggtgtaata agtgtattta
     6481 taggtgcagg tcttgggctt attccaggtt gtggtataca aattgtgctt atgtcatttt
     6541 atttgaaggg aactatacca cttggagcag tgatagccaa ctctatttct caagatggag
     6601 atgcactttt cccactttta gcaatggata aaaaatcttc tttatgggct atgattatta
     6661 ctactatacc agcaatactt gtaggtatga tagtttatgc atttttaggc taaatataat
     6721 tataaataaa gacgtattaa aaaaataata aagcacataa atttatttat aagaatagtg
     6781 aagattaagt atagaaataa aaatttcaat atgaattaaa ttattgctta aatattaatg
     6841 agaacatggg agataatatt attcctcatg ttctctctat ttaattaaag gaattataga
     6901 acaaattt
//
LOCUS       DAEMAE010000196         6866 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEMAE010000196 DAEMAE010000000
VERSION     DAEMAE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.198"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            47..1171
                     /locus_tag="KPH93_003041"
     CDS             47..1171
                     /locus_tag="KPH93_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG1689815.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNMVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIFLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLENNTSIQSKQHSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     HIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESKFWQKYEGKID
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1645..2469)
                     /locus_tag="KPH93_003042"
     CDS             complement(1645..2469)
                     /locus_tag="KPH93_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689816.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTSVYLASTVS
                     TITRKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLEIYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(2817..3872)
                     /gene="ispG"
                     /locus_tag="KPH93_003043"
                     /gene_synonym="gcpE"
     CDS             complement(2817..3872)
                     /gene="ispG"
                     /locus_tag="KPH93_003043"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG1689817.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATTEALVESAMGH
                     IKILEDLDFHNIVISLKSSNIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(4055..5059)
                     /gene="rseP"
                     /locus_tag="KPH93_003044"
     CDS             complement(4055..5059)
                     /gene="rseP"
                     /locus_tag="KPH93_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG1689818.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(5072..6226)
                     /locus_tag="KPH93_003045"
     CDS             complement(5072..6226)
                     /locus_tag="KPH93_003045"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG1689819.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHSDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEILPKNIEIEVLAGMEGLKIISSLDEVDILLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(6390..6593)
                     /locus_tag="KPH93_003046"
     CDS             complement(6390..6593)
                     /locus_tag="KPH93_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689820.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         2291 a         1205 c          714 g         2656 t
ORIGIN      
        1 ttgatatata atatgtttat ataaactaat gtatgtgagg tgaaatatga gcaaaaaaac
       61 aaaatatttt tctttaattc tctttcctat tgctttgctt ttaaatttta tagccagcaa
      121 agttccaaat atggttgaaa aatattactc ccaatttata gataaaataa ttgtacaaat
      181 attaagtaaa gtttcaggaa ttttcccatt ttctctctat gaaattacaa tgtacataat
      241 agttatatct atcttcttat ttttatgttg tactttatct acaattttta ataagaaaaa
      301 aaaattaaac atatttttaa aaaattctat tttaaatata ttatccattg tatcaatagt
      361 atactttctt tttgtagtgc tttggggaat aaactataat aggattccac tagaaaccac
      421 acttataaat aactataatt tagaaaacaa tacctctatc caatcaaaac aacatagtgt
      481 taaagagctt actaaattat ataaattcct tgtaattaaa gcaaatgaaa ctagaaaact
      541 cacattacaa gataaaaatg gtattgtaaa atctaataca gattataatg gtgtcataaa
      601 tagagctcaa ctaggatacg ataatatctt ggatatatta cctagtgtta gtggtagtta
      661 ttctaagcct aagtatatta tttcatcaaa tctaatgtgc tatacaggga taactggcat
      721 atactttcct tttacaggtg aggcaaatgt aaatatagca atacctgact tgtatattcc
      781 ttgtacagtt gaacatgaaa tggcacatca aaggggattt gctagtgaag atgaggctaa
      841 ttttatagcg tatcttactt ctataaaaca cccccatatt gattttaact attctggata
      901 tattttagct ttaaattata ctgcttctgc tttaagtaag gttgattata atgcatatgt
      961 tgatatttct gctggtattt cagattctgt ccgtagagat ttaaaaaatg aaagtaaatt
     1021 ttggcaaaaa tatgaaggta aaattgatga gatttctaat gagtttaaca attcttactt
     1081 aaaagctaat ggcgttactg aaggcactca gagttatggt aaaatggttg atttattgct
     1141 aacttattat gaattatatc catataacta aacactacaa ttcagaactt tattcatata
     1201 cagctggtat taaatcagct gttttctatt taaataaatt atttagctcc aagcaatctt
     1261 atataatttg aaagtactta tactatctgt ataacatttc aattctaact attttagtta
     1321 aaacatttct attatctttt ttgataaatg ttttaactaa gatttttaaa acataaaact
     1381 tccatattat ttcactatct atataaaaag ctaaaaattt acatataaaa ttacataact
     1441 aaaaaatcat aaatttatag aaaacatata ttcaaaataa aaaagcatgt atcaaaactt
     1501 gacacatgct tgtaatctta tgtatatacg ccattttgtc acaataaaaa tttccttgta
     1561 acactgacat ttttaaacaa aatagttctt atttaattaa attaacatta gttaaattcg
     1621 tacttatttt gctatcatta aacattactt atagtaattt tctaatacac tatttgaaac
     1681 ttccttccct tgaaaacttt tcaaccaatt ctcatatgct gaatttttcc atacactacc
     1741 atcattaaat gttacttgtt ttacacaaga tataatgtac tttacaccat gctctccagt
     1801 tgattgtttc catttatcaa acaatagaaa ggcatcattt tgattcttac ctggtgctat
     1861 tgcattttgc tttgcatatt ctttctccat ttgtttccat tctttcaacc aattttctac
     1921 ccatgccttg ccttgttgtt tttcaaacat acttattata tcctgtggtg gtgattgttg
     1981 catttttcta taagtatgtg aatatgattt tggagacaca ggagatatgc ctgtaacaat
     2041 accatagtct acaccagctg ggctaaaccc aacacttcct acttcgccat ctgctgccac
     2101 attttgagca tcccaatata tctcaagtgg ttttccattt ttatcatatg ctaagcacgt
     2161 aatttcataa tccataattg ctttttcact gctatttgtt aagcttataa cattgctagg
     2221 ccaatgatat ggagtatcat catactgtat tttgctatta tttataaata atggaatatt
     2281 ttcattgtaa acaagacctt tactactttg aatatctgat actttccttg tgatagtact
     2341 tactgtactt gctaagtata cacttgtttt ttctgaattg gtcgtactaa gagaaaatgt
     2401 tgttgtcgtt ccagtaatca gcaaagcaac aactaaaata ggtaagctag atattttttt
     2461 atatctcaca gttcttcacc ccttcttcta ttaacttata ataaaacaat ttaccaaaac
     2521 attaaaatct atgttaacat aatttaccat atgttctagt aactatatcc tatcatagtt
     2581 actatcaagt ttcaatacta ttgatggaaa ttttcactat attaaatctt tttttaattt
     2641 tataaattta caatatgatt taactcatac atatatagtc aaattagaat ataagtttct
     2701 tacatttaat gaagtttata aaataaaaaa tgactagata attaaatact gtaaataaaa
     2761 actcattaca aaagtattta aaaatctagt caattaatat atatctgaat tgtatactat
     2821 aatttctcaa tctcttctag caactcatca actaatttgt taccatcaat cattctcact
     2881 atttcaccct ttttaaataa aagaccttca cctttacccc cagcaatacc tatatctgct
     2941 tctctggctt caccaggtcc atttacagca cagcccataa ttgcaacagt gatatttttt
     3001 tccatactac caatcttttc ttcaacctca tttacaacac taattaagtc tatattacat
     3061 ctaccacatg tagggcatga tataacttta atcttatcat ttaaaagtcc caaacttctt
     3121 aaaatttgtt taccaacaat aacctcttca acagggtcgc ctgttaaaga tattctaaca
     3181 gtatctccaa ttcctttgag aagaagtgct cctaccccta tagaagactt tattgttcct
     3241 ttgtgaacac ttccagattc tgtaattcca atatgaagag gataatcaac tttttttgat
     3301 attaattcat atgcatcaac agttttataa atattggatg attttagaga tataactata
     3361 ttatgaaaat ctaagtcttc aagaatctta atatgtccca ttgcactttc aactaatgct
     3421 tcagtagttg cagagccata cttttttaaa agttctttct ctaaagaacc tccattgact
     3481 ccaattctaa tttttaaatt tctttcctta catttctcaa ctaccatctt aactctctct
     3541 atacttccga tattaccagg atttattctt attccatcta caccttgttc tatagcttca
     3601 agtgctagct tataatcgaa atgtatatct gcaataattg gtatatttac actagacttt
     3661 atctttgcta tatttttagc agctttaata tcaggaacag caactctaac tatatcacaa
     3721 ccagcttcct ctagtctctt tatttgagct attgtagcat ctgcatctct tgtatcagta
     3781 tttgtcatag attgtatact tataggattt tctcctccta tttttacagt tcctacactt
     3841 acttctctcg tctttcttct ctcgtaactc atattccacc tctatatatt attttatatt
     3901 gaatctcttt aaatattaaa atattataac ctacatgtat attataaatt aaaaagctat
     3961 ctagttaaat aagaataaat ttcatacata aaaattagtt tattcttatt taaaactaca
     4021 tagcctattt tcttaaaatc tctaaaaaac taaatcattg aaataatctt agtatatctt
     4081 tatatgtgat aaaaagcata aataacatta aagctccaaa accaacaaca tttatcatgc
     4141 cttctttatt tgggtcgagt tttttaccac ctctaacagc ttctagtaat agcataagta
     4201 ttctccatcc atcaagtgct ggtattggta atagatttac tatacctaaa tttaagctta
     4261 tgacagctgc taaatacact acatttatga tgcctgtacg agcagcatcc gataccatac
     4321 caataacacc tacgggtcct gctacagcat tgccaatccc tcctggtaca cgtcctgtaa
     4381 tcatttgacc tagaaatgtt agcatctgtt ttgtcatatc tacagtagtt ttacatgcat
     4441 ttactattga ccccaaaaag tctttttctc tttgtggttg aatccctatt tcatactttc
     4501 cattgttatt tttaggtgtt acatttacaa ctttatcact tccatctcta gttatactaa
     4561 gttttaattc tccaccagaa gaagtattta ttatatttgc aacttcatcc caagtcttaa
     4621 cagaattacc attaatttta tttatcttat ctcctacttg aagccctaca gcctgtgcag
     4681 gagtatctgg cattatcttt cctaatttag tcgttggaga acctatatac aaaaatactg
     4741 gtactaaaag tattgctgct aatattatat tgaatattgg acctgctaca atagtactaa
     4801 atctttgtaa tatagacttg tttccaaaag aatttggact tatttgctct tcatcttcac
     4861 cttccatact tacatatcca ccaattggaa gtgctcttat agaatattct gtatcttttt
     4921 ttacactata tatcttaggc cccataccta tagaaaactc atttactttg ataccacttc
     4981 tttttgcaaa tataaaatgt cctaactcat gtattaatac aattatacta aatagtatta
     5041 atgcggctat tatagtcata aattcacctt cctatttttt tatcagattt gcaacataag
     5101 ctctactcca tctatctgtt tctaagattt cttctaatgt tggttcactt atgctactat
     5161 gaacttctaa agtcttctct atgtaatatg gtatgtcata gaaaccaatt ttatcctcta
     5221 aaaattcact tactaaaact tcatttgctg aatttaaaac agaagaataa gttcctccca
     5281 tttttaaaca ctcatatgcc aattttaaac atggaaaagt ttctaaatct ggttcttcaa
     5341 aagttaaagt actaaatttt gaaaaattca ttctttcaaa gctactttcc attctatctg
     5401 gatatgtaag agcatactgt attggtagtc tcatatctgg acatcctaat tgagcaatta
     5461 ttgagctatc tgcatactgc accattgaat gaattatgct ttgtgggtga actactacat
     5521 ctatattttc ttgttctacc ccaaacaacc atctagcttc tatcacttca agtcctttat
     5581 tcataagcgt tgaagaatca atacttattt ttcttcccat actccaattt ggatgcttca
     5641 aagcttcatt tttagttata tttacaagtt ctcccttttt ctttcctcta aatggaccac
     5701 cagatgctgt aagtattatc ttttctatat tttttttatt ttctccattt aaacattgaa
     5761 atatagcact atgttcacta tcaacaggaa gaatatttac attgtatttt tctgcttctt
     5821 tcattacaag ttcaccagca gttaccaacg tttctttatt cgctaatgct atgtctattc
     5881 ccttctttat agcacaaaga gttggaacta atcctatcat acctactaca gcagttagaa
     5941 ggatatccac ttcatcaagt gatgatatta tttttaatcc ttccattcct gctaaaactt
     6001 ctatttctat atttttaggc aatatttctt ttaatttcaa tgctttattt tcctcaaaaa
     6061 ctgttacata tttaggttta aactctacta tttgttctag caataattca atattactgt
     6121 tagctgatat tgcaactatc tcaaacttat cactatgttc tctaaccaca tctaaagttt
     6181 gtttacctat agagcctgta gaacctaaaa ttgatatctt tttcatataa taatcctttc
     6241 taataataaa tataccctgt caaaacatta taacactatt aaaacaatta tttttactat
     6301 tcatagcaat ttcatatatt attccacaga ttaaaattaa tctattattt tttgtatttt
     6361 gtggaatatt ataaaatatt ctagtattat taaatgccct tttttatttc cacaacatct
     6421 tctattttac agtctagtgc taaacatatc ttctccattg tatccataga aacagattta
     6481 tcatttccta gtctgcctaa aacagaaata cttatacctg atatatttac aaattccatt
     6541 ttctttattt ttctgtcaat taacaatttc cataatggag cgtatgaaat catgttatca
     6601 cctcgcaaaa tatattctat cacattttat ctattttgga aaattttcat ctatttagat
     6661 aaataattat tttatctaaa tagatttcaa ttatagttta ttaaatataa atatgttaaa
     6721 ataaaacaaa accataagtt taaaaattaa gctttatata tatttatttc attatgttac
     6781 taaaaaaata actatataaa aaatatttac tattatatat ctatttaaaa cacattattg
     6841 tagcgtcata taaacatatg caaata
//
LOCUS       DAEMAE010000197         6818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEMAE010000197 DAEMAE010000000
VERSION     DAEMAE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.199"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            5..697
                     /locus_tag="KPH93_003047"
     CDS             5..697
                     /locus_tag="KPH93_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689821.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     NIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            893..2962
                     /locus_tag="KPH93_003048"
     CDS             893..2962
                     /locus_tag="KPH93_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG1689822.1"
                     /translation="MDKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDKGTMASLLPTFKLSVGGKLTSKEKGKFQQEGKSTDLSSSDSE
                     YDKIMINEQASEEYLGYLKNIKVYVINTYTGVFYTNTSYSNLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELDEVYEVDEGFEAYISFPEEPTIEDGIVYTNFQ
                     IFKQATEKVRLFFVASLSLAIICLLSIILYSKIKGEVLKRNNVFLLIYSKIPMEFNVI
                     LVSISIAILIMYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFQTSI
                     IVRLILWLIKTVREISNAIKKVSLARKVIVIVIISLALIMVISTLSIGIGLLFSICIF
                     LGFTIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRA
                     IEEQVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKS
                     KRLKLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPN
                     DKVYIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNF
                     DPSEIMERFKRADESRNTEGSGLGLAIARDLVDLQGGKFYIEIDGDLFKANLEFQKYE
                     EEITNIE"
     gene            complement(3168..3491)
                     /locus_tag="KPH93_003049"
     CDS             complement(3168..3491)
                     /locus_tag="KPH93_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG1689823.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSIYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3484..4185)
                     /locus_tag="KPH93_003050"
     CDS             complement(3484..4185)
                     /locus_tag="KPH93_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG1689824.1"
                     /translation="MERTQQLRESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDKNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4463..5848)
                     /locus_tag="KPH93_003051"
     CDS             complement(4463..5848)
                     /locus_tag="KPH93_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1689825.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEINTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTADSLLPASGGQNAIVAILAGLFQHGDRVGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYTCKNENIKGLYIIPDYQNPTTHIMSQNGRKMVANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMIKSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEDEK"
     gene            complement(5956..6099)
                     /locus_tag="KPH93_003052"
     CDS             complement(5956..6099)
                     /locus_tag="KPH93_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689826.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(6295..6717)
                     /locus_tag="KPH93_003053"
     CDS             complement(6295..6717)
                     /locus_tag="KPH93_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG1689827.1"
                     /translation="MNTFKTEVLLKYSAKDIFNTFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESIITRYELVHIDLENTKLIFIESESERNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         2607 a          790 c          945 g         2476 t
ORIGIN      
        1 aaatatgtac aatatattag tagtagacga tgataaagaa atcgttgatt caatagaaat
       61 atatcttaaa tcagaaggat ttaaaatata taaagcatat gatggaatga aagctttgga
      121 aataataaca gagaataaca ttcatctaat actaatggat ataatgatgc ctaaattgga
      181 tggaatcaaa gcaactgtaa aaatacgaga agaaagaaat attccaataa ttttaatttc
      241 agcaaaaagt gaagatacag ataaaataat aggattaaat attggagctg atgattatat
      301 aacaaagcca tttaatctat tagagcttat agctagagtt aaatcaaact taagacgata
      361 tgtaacttta ggtaattatg aagatacatg taaagaaatt ttaaaaagtg gagctttgga
      421 gttaaataca ctaacaaaag aagttaaaat tgatggtgaa aacataaaaa tgactccaat
      481 tgagtataaa ataattgaac ttcttttaga aaataaagga cgagttttct caatagatga
      541 gatatacgaa aaagtatgga atgaggaaag ctttaattca gaaaatacag tagcagtaca
      601 tatcagacga ataagagaaa agattgaaat aaatccaaaa gaaccaagat ttttaaaggt
      661 ggtgtgggga gttggttata agattgaaaa attttagtgg aaaaattata aaagaaaaga
      721 gtttaatatt taattgcatt ttgattttag tcatttttac atttacacaa atgttaggaa
      781 ttttaatatg ttttaatttt ttctctgaga gtttaattta ctctatgggg attatgcttt
      841 tcttaaactt tattttatat aaaatactga caatgtagat gaggtgggca atttggataa
      901 atatagagta ttaaagttag tttcattaat cataatatct atctcatttc cattaactat
      961 attgttggtg acaataggac ttgctgagcc taaatacaat atacaaaaaa cttctacaaa
     1021 gagtgggatt tttgatatat ctgatgattt ttatgatagt aatatatttg acaaaggaac
     1081 aatggcaagt ctattaccca cgtttaagct atctgtaggt ggtaaattaa ctagtaaaga
     1141 aaaaggtaaa ttccaacaag aaggtaaaag tacagattta agtagtagtg attctgagta
     1201 tgataaaatc atgattaatg aacaagcttc tgaagaatat ttaggctatc taaaaaatat
     1261 aaaagtgtat gttattaata catatacagg agtattttat acaaatacgt catattctaa
     1321 tttatatgat tttaaacaaa aaactaagag ctattgtaat gtagagataa taaataagat
     1381 aggaaagagc tcatatatta aaacaataaa tggagaaaaa tttgaactga ataatttgag
     1441 tctggaacta gatgaagtat atgaagtaga tgaaggtttt gaggcatata tatcttttcc
     1501 agaagagcca actatagaag atggaatagt atacactaat tttcaaatat ttaaacaagc
     1561 tacagaaaaa gtaagattat tttttgtagc tagtttaagt ttagcaatta tatgtttatt
     1621 atctataatt ttatatagca aaataaaagg tgaagtttta aaaagaaata atgtattttt
     1681 attaatatac agtaagatac ctatggaatt taatgtaata ttagtatcta tttcaattgc
     1741 aattttaatt atgtatgccc ttgctgtatc ttatcatatt gatgtaattg cattagtttc
     1801 tttatcttta ctgatagtat caaataatat attttttata aatgtgcaat tgagttattt
     1861 agaaaataaa atgtgtatat ttcagacaag tattatagta agattgattt tatggttaat
     1921 aaaaactgta agggagatca gtaatgctat aaagaaagta tctttagcaa ggaaagttat
     1981 tgttatagtg ataatatctt tagctttaat catggtaatt agcactctaa gtataggtat
     2041 aggattactg ttttctatat gtattttttt aggatttaca atatatataa ctaaaagact
     2101 ttcatatctt agttacgtaa tagaaggtac agaaagaata aaaaagggag aattagatta
     2161 taagataaaa attactggga atgataattt tacgtcttta gctgaaaata taaataatat
     2221 tggagaaggt cttgatagag ctatagaaga gcaagtaaaa agtgaaagaa tgaagtctga
     2281 ccttataaca aatgtatctc atgatttgaa aactccactt acttctataa taaattatgt
     2341 agatttaatt aaaaaagaag aatcaataca accagagtat ataaatgatt atataaatgt
     2401 acttgagtca aagtcaaaaa gactaaaatt attgatagaa gatttatttg aagcgagtag
     2461 agtaagtagt ggcaatatag aattacaaat tagcaagatt gatttagtgc aactattaag
     2521 acaaagtata ggtgaacttg aagaaaaatt atctaaaaat aatttatatt taaaattaaa
     2581 tgttccaaat gataaggtgt atatatgggc agatggaaga agaatgtata gagtatttga
     2641 aaatctttta tctaatatag caaaatattc cttagaagaa actagagtct atattgatgt
     2701 atatgactat ggtgaaaatg taaaggtaac tatgaaaaat atatcttctt atgaattaaa
     2761 ttttgaccca agtgagatta tggaaagatt taaaagagct gatgaatcaa ggaatactga
     2821 aggaagtgga ttagggcttg ctattgcaag agatttagta gatttacaag gtggaaaatt
     2881 ttatatagaa atagatggag atttatttaa agcaaattta gagtttcaaa aatatgaaga
     2941 agaaataaca aatatcgaat gagatagtat ctaagtatat atattttttt tactgtcgaa
     3001 agcttttatt ataatatatt agaatattta cagtagagaa tgaagagtaa aaacagagta
     3061 actttaagtc actctgtttt ttacttaaaa tcaataatat aacaacttaa attaaaattg
     3121 tacaaatagt tttaaaataa cttatacaat aaaacattat ccaaatatca taagataatt
     3181 ccaaataaat atatagatat cactcctgta ataatagaaa taccaataga tttaaactta
     3241 tatgcaacaa gaagtacaat cataaaagat ataattttag ggttagctat agataaatat
     3301 atactatctc cattaaaaaa tacatcttta gcagttaagg aagtaagtat tgctacaggt
     3361 ataaatttca tgattttttt caaaaaatca ggcatctctt tttttgaaaa aattaacata
     3421 ggaagcactc ttggtagata tgtaacaatg tacattccaa ttataataaa aaatatataa
     3481 ttattattca taagattcct cctttttaca gtaagattct tctataaaga aaccaattaa
     3541 agatgctaaa attgatgcaa ctattattac taaattgctt ttgaataatg catataaaat
     3601 aacagaaata atgattgatg atataaatac tacaatataa atcttatttt ttatttgaga
     3661 aactattaaa gctataaaca tagaagtcaa tatgaagcca gaaatagaac tgtctataga
     3721 tatactttct cctaaaaatg ctccaataaa attagctaaa actgatgaaa ttaagcagaa
     3781 gaaattgaga taaatagcat ttttatcaga ccaatttcca gcagtaaatt tttcataatt
     3841 aactgcaaaa gtttcatctg ttatagtatg acaaaatggc aatataaatt ttggactttt
     3901 attttttatg tacatgttca aattagaact cattagaaaa tgtcttaagt taactataaa
     3961 tgtagtcaaa acaatagatg ttacacttgc accagtagaa atcattgaag ctgcaatgaa
     4021 ttgagaactt cctccaaata ctaaagttac cattagtgct atttgaaaag gtgaaagacc
     4081 agatttttgt aatagtattc ctcctactat acctataggt atataaccta agaatatagg
     4141 aattgaagca ataaagcttt ctcttaattg ttgagttctt tccacaataa atcactcctt
     4201 ttgtattaaa tgtattaaag tattagtaaa acagaaaaat agattaattg tataattgca
     4261 attataatta aaaataaatt tggattaata tatagattac tatataacaa atagataatc
     4321 ttaaactatt attaaatttt atctatttgt tattatgatg taataataca tactaatctc
     4381 tataaattaa ttactatctg gtgtaatttt attttaacaa caaaaaaaaa tacacacaat
     4441 taaaatattg tgtgcaatac aattattttt catcttctaa tagtcttttt aatacgctca
     4501 gaccagcttt aagttcttct gtgctttctg tagcacatac agctattctt attgcagaaa
     4561 ttggtttttc ttttccaaca gcaaaacgtt cagaagcata gacttgtaca ccttctttaa
     4621 gtgcttgtat ttcaaatttt tctgccatta ttccatcagg cagaatcaac catctaaata
     4681 tacattcttc atttcccaaa agattatatc cagataaata ttgattaata atcttatttc
     4741 ttcttcttgc agatttacgg cgtgcattaa ttaatttatc ggcttcacct gatttaatca
     4801 tacgataagc aatttctgtt aaaaaataag attgagataa attaatgttg tataatgcgc
     4861 ttgataatgc ctctcgatac tgtttaggtg tagagatata tgctagccgt aaactaggtg
     4921 caataatttt tgataaactc gtaatataaa ttgtttgatt gggcagatag gaagccactg
     4981 gatttaaatg tgtttcatta agtaaactat gaatagcatc ctcaataaca attaaattgt
     5041 atttagatgc aatatttgca accatttttc gtccattttg tgacattata tgtgtagttg
     5101 gattttgata atctggtatt atgtatagac ctttaatatt ttcattttta caagtatata
     5161 atagtccttc ttcactaatt tcattatctt cttgtttaat tggaattagt tgaatcccaa
     5221 gcattttagc tgctgtcttt attccaggat aagttaatgg gtctacacct actctatctc
     5281 catgttgaaa caaacctgct aaaatagcaa caattgcatt ttgcccacca cttgcaggaa
     5341 gtaagctatc tgctgtagtt tcaagcccag ctttataaat taatttggca atagcttctt
     5401 tttgccattt agcaccatct ctttgtccat attggaatat agttttaaaa tctatttctt
     5461 taattatgtg tttaaacaaa gtattaattt cttctagtgt gaaatctgga ttcattgtgc
     5521 ccatttcaat aaatgtaatt ttattattac tggacatcaa gaatagattt gaacgcgtat
     5581 cataagatac aaaagttcca cttccagtaa ctccacttat tagacctttt ttttcacaaa
     5641 ttttaaaagc tctagagata gtactcacat taacatctaa gaaatctgcc aattcacgtt
     5701 ggggtggtaa tttagtacct ggtaataaaa atccattgtt gatatcttgt tctagttgtt
     5761 ctgcaagagc cttatataat atttgccctt ttgaaggtct ctttggtttc cagttcatag
     5821 gataattttc aaaagaatta attggcataa gttttcacct cacattatta atttgatttt
     5881 ttctttatct aaaacaataa attctaagga tacaaataaa aactgatagg aaataattct
     5941 atcagttttt gtcttttaat tgatttcaaa tcttcctata atttttatac cttctttatc
     6001 ataaagaggg attgtttttt taaatatatt aaaatttctt tttcttgtat ataaaacagc
     6061 ttcttctggt gtctttggtt gattatcaca atcgtacaaa tctctttttt ttaaactaaa
     6121 agcaataata cataaaatta ttattgctaa tgatactcct aatgtggtat atattcatag
     6181 tgtcacatgt ctatcaatgt ttttttcagt aattattgta acaaatagta gaacccataa
     6241 aatacttaaa aatatgtttt tagatagctt ttaaatatat aatgtaactt atatctataa
     6301 attaacacta ttattctcta attccatttc tagttttttt ataaatttat taaattcttc
     6361 tggttgcctt tttccaaaca atactcttac aagagctgaa ttgatttttc caaatatatt
     6421 tctctcgctc tcactttcaa taaaaataag ttttgtattt tctaagtcaa tatgtaccaa
     6481 ttcatatcta gtaataatac tttctctttt gttgcttgtt tttatctcat atactctatt
     6541 tttttcgaaa ttagttattt caaccttaaa tcttttatta tttttttgtt gtacaaatgt
     6601 gcctactgct tttttctcat taaaattagg aaaatttagt cttgcagatt ttgtaaatgt
     6661 attaaatatg tcttttgcag aatattttaa taaaacttca gttttaaatg tattcatatc
     6721 gttttccctt ctgtatataa atttaatatt tataagttaa tttttaagtt aaaatagtat
     6781 aatatgccat attaaatcat gttaaaacta tttatata
//
LOCUS       DAEMAE010000198         6672 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEMAE010000198 DAEMAE010000000
VERSION     DAEMAE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.200"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(237..1673)
                     /locus_tag="KPH93_003054"
     CDS             complement(237..1673)
                     /locus_tag="KPH93_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG1689828.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIILQGDSNLSIGATQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVDMSNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRIFKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFGSMKDSYFS
                     GIIRRCSQYYYNTDYDTYLNIKDKVEEAYSLFYGNYFTECNIDLISDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNDKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEASYGEKW
                     EKLGSEVIGKETIYYYKHHEPDKTNQLQ"
     gene            complement(1883..3283)
                     /locus_tag="KPH93_003055"
     CDS             complement(1883..3283)
                     /locus_tag="KPH93_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG1689829.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDKKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALETKDVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     EEYYGDKVNIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            complement(3310..4656)
                     /locus_tag="KPH93_003056"
     CDS             complement(3310..4656)
                     /locus_tag="KPH93_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG1689830.1"
                     /translation="MENEAKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGA
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDSQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTSVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMLYSQITARITKCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNMDTLESDLLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEVCSVGGVQISDIDEKDLIKIIENTLYID
                     NKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDIKVLAGESMYYYKKIKD"
     gene            complement(5026..5700)
                     /locus_tag="KPH93_003057"
     CDS             complement(5026..5700)
                     /locus_tag="KPH93_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1689831.1"
                     /translation="MNDELKSILYSKGVDIVRFVDISEFPTNQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQSKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLINSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            complement(5761..6507)
                     /locus_tag="KPH93_003058"
     CDS             complement(5761..6507)
                     /locus_tag="KPH93_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG1689832.1"
                     /translation="MKIENLSIDDIKRGYIFNADLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQIG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
BASE COUNT         2194 a         1191 c          613 g         2674 t
ORIGIN      
        1 actgattcat ttctagcatt taaaatatcc ttaataagtt tataattctt taaaaataaa
       61 tatatattat tcctaaactt ctaaaactaa ttttataata aaattaaaaa aggtgctatt
      121 ttcaagcacc ctacaatttt actttttaac aaatttaata ctctacaaat atatcaatat
      181 tacattaaag gtaaacacta tatattaaaa ctttatatca ttatataata caaattttat
      241 tgtagttggt ttgttttatc tggttcatga tgtttataat aatatatagt ttcttttcca
      301 attacttcag aaccaagctt ctcccatttt tctccatagc tagcctctat ttgttcacat
      361 tctgaatctg ttgatgctac aataagacca taattaggtc tgaatatagc tgtttcttcc
      421 tttccatttt tatagcttaa tttatcatta tttagcctta acaagtttcc attcttttga
      481 taattaaaat taattccatt aataacttct ggactagatg aatcaataaa tatataattg
      541 ttaaagttat tttttaacat taaattgcct cctccatgta ccgatttaac gatataatca
      601 taggcatcta tattgcatct caatgttaat tttggtattt gaagggtttt aaatacataa
      661 actttatgtt tatattctaa gccatcatcc cagttatcct tattatcact aattaaatca
      721 atattacatt ctgtaaaata atttccataa aataaagaat atgcttcctc taccttatct
      781 ttaatattta aatatgtgtc ataatctgta ttataataat actgactaca ccttcttata
      841 ataccagaaa aataactatc tttcattgaa ccaaatgaaa agccacaata cccacattgg
      901 tcaatcgtaa tattattgat tttataactt ccatacccct catacccctt aacttgacta
      961 cttataagac caactttctg tattttttca atccttacat tatttatact agttccagtt
     1021 ctaatcctca ttccattttg acatttaaat attctactat tagaaataat tgtgtctatt
     1081 cctgtatcaa tacccagccc acaagatgaa actgtaatgt catttacaat tgagtaatat
     1141 ggtatcctaa ttcctgtacc cgaaaaacca cttatataaa tattttcata atgacctaga
     1201 cctttaatca tattcttact gttatcatac aggattgctg atacattgct catgtcaaca
     1261 ttcttttcat tcttattaat aatcatctca taatgatatt taggattacc ttttgtcggt
     1321 ggttcttcat ttacctgtat ctcacaacta tcagaataaa aatttatatt tttaatacat
     1381 tgttttttcc ctgtcaatgt aataatactc atattttcta ctccacgtgt tataaattgt
     1441 gtagccccta tagataaatt agaatcacct tgcaatataa tttctttatt gattttaaca
     1501 ccacttgtaa ataagtactt accttttgga aaatataaga cacaaaaatc tgtttctatt
     1561 tcatcaataa tatcctgcaa atcaggacct acgttgtacg aaccatcatt tttaatacct
     1621 ctttctacta cattaatcca tcttccatct ttaatcccag ttcttccttc cataatatta
     1681 tatattcctt tcccatttac ttaatttgaa cctaccatga ctaaagtcac aggtttacca
     1741 cttcatagaa ttttacataa tcaaattaat tttttatatt atttttatat tattcaatac
     1801 acactattac caaaccattt acattaaaac tatagtccta gctctccaat aaaatacatc
     1861 tattcaaata ctacgtattt acttatttct ctatcaattt ataatagtgt acagttattc
     1921 caaactctat tttttcacca attttctccc atgttccccc ataatgagcg gaaagtatat
     1981 tttcatcttt aataattgtt gacataatgt cacctacctc tggtttatgc atcttaataa
     2041 ccttatcctt actatttata tatatatttc ctttttttat atttacttta tccccataat
     2101 attcttcaga tataccaatt ccattgatac tacttagttt tccatcgtaa aatataacag
     2161 tttgtcgcat attattgtat gttaaatttc ctttttctga cttaacaatc cagtcagtag
     2221 catctatatt gcatgttaat agtacatcct tggtttcaag tgcttctata agatacacct
     2281 tatgcttatc ttctttgaaa tcttcccagt tatcttcatt agtatttgac aaaactatat
     2341 tacttgactc caataaatta ccatagaata aagaatatgc ttctttaacc ctattatcca
     2401 acattaaata attgtcatag cttacatcat aatagtactg cccacacctt gtgatatttc
     2461 ctgatatctg aacattggat atagaatcaa accaaactcc acaatatcca cattggtcaa
     2521 ttgtgatatt tgtaatgaaa tttaaacctc taccaatatt tttaattcca acttttttta
     2581 tttcttctac cctcatacca tttataaaag ttccagtaga aattatcatt ccatcaacac
     2641 agccccaaac cttactgtta gcaatcgttg aattttcccc tgtactaatt ccaagtccag
     2701 atgtaaaaac tgtaatatca tttgctgtag aattattagg catatcaagt gcaattccag
     2761 aaaaaccact aaaagacaga ttttcataat gacctggttc tccctgttta tcttcacata
     2821 caatagctga tatatttcta taatttataa ttacattatg atgaaatcta ggttttttat
     2881 ctgttggagg ttcatgatta gatgtcatat ctctatcaca actatctgaa taaaaacaga
     2941 tactcttaat acagtgtctt acactttttg ctgttataat tgctatattt tcacttttag
     3001 gatttttctt tcctttaaaa gtaaattgtg ttactccagc agtgttttta ggaacattcg
     3061 ccaaaatata agaatctcca cataaggtta atcgtttctt aatcttaaca ccactttcaa
     3121 ataagtattt tccttttgga aaatatataa tagaatcttc tggtgcagca tctataatct
     3181 cttgcaactt ggaacttaca tcttttgttt catcattaca aatcccataa tcaaggacat
     3241 taatccagcc acttatatct ttaattatta tagactcttc cattaatcac tacctcctag
     3301 tttgataatt taatctttta tctttttata atagtacatg gattccccag ctaatacttt
     3361 tatatcaatc tgttcccata ttccaccatg taatttattt atttcttctg tagaatctag
     3421 tactgaatat attacagtat tcttttttaa tctaggaacc attaaatact tacctttatt
     3481 atctatatac agagtgttct cgattatttt gattaaatct ttttcatcta tatcagaaat
     3541 ttgaacacct cctacagaac aaacctcacc attataaaat ttataagtat ttacaccatt
     3601 ttctaacagc aagttacctt ttgcacattg tataaagtta tctgcttcag cataacatgt
     3661 tagcattaca ttgtttgttt catttgcctt gattacatat atcttatgtt catcaagtaa
     3721 atcactttct aaggtatcca tattactatt catcaataca atattgcaat tttctaaaat
     3781 atctccataa aaaatggaat aagcttcttc ttttctatct tccatattct cataagcatt
     3841 ataatcaaca cttttataat actgaccaca tttagtaatt ctggcagtta tctggctata
     3901 gagcattttt tcaaacataa atccacaata tccacattgg tcgaccataa gattcataat
     3961 caggttaaaa ccaaatcctt tattattaat acctatttct cttatttctt ctattctcac
     4021 attatttaag ctaacactag tagtaatctc cattccattt ttacattcgt aaatcttgct
     4081 actagaaagc attgacttct ctccaagcct cataccaaga ccacatgaac taacaatgat
     4141 atcattgcca gttgaatagt atggaatttc aatccctatc cctgaaaatc cagaaataaa
     4201 cagatgttca aagtgaaata gtccttgtgt ttctccatat tctgtaatag cagatacatt
     4261 tttaggtggt attttctcta tatcatgact atcataataa aaattaatat ttttgataca
     4321 ttgacttgtt cctgtcaatg caataatact catattagaa actcctcggg ttataaaatt
     4381 ggttgttcct attattgatt gttttttatc taaatttgat acttgactat ctcctccata
     4441 ataagaatct ccttgaagtg ttatttgttg actaatttta ataccttttt caaataaata
     4501 attacctttt ggaaaatata taatggcacc ttctagtgac ttatcagaaa caatattttg
     4561 tatcatatta cttacatcat atttcccagt aatatctatt ttactgttta attctagtac
     4621 attaatccaa tctggagttt tagcttcatt ttccatgatt aattcctcct tttaaatcta
     4681 aattcttttc acagaaacaa atttttttga ttataactga agtgactttt actctctttt
     4741 ggaattttga gaggacaatt ttcattttta taacatcatt atagttctat tataaatcaa
     4801 ttataatttc attatatttt tactattacc aagttattct taatttttta atacaaattt
     4861 tatataatga ttaaaatagt taagctttgt atcaatataa tttaatccca ataaaaaaac
     4921 actcatttct gagtgttcta tacaagtttt tattttgaga catcttatat aatcttatat
     4981 tttaaaaagt ttacaatatc caatttaata tcttctctag taaagttatt tttgatttgc
     5041 atatcttaat gaccatgggc aactcatcat acacttcaat acacaaaaac actttgaaac
     5101 atcaataata gattctcttt ttcctggcaa agtccactca tttccatgta tagcttttgc
     5161 agggcagttt tctacacaaa cattacaatt tagacatttt gaattaatca aaggatatct
     5221 ttcaacagaa attggagcat ctgtaagtac tgtacacata ctaaaagcac atccgtattc
     5281 ctctgttaca aacaaattat tttttcccat aaatccaatg ccactaatat ttgctattgt
     5341 cttatgtggt aaagggctaa ttcctgattg catttctgga ttaatatagg ctttttcaaa
     5401 atatccatgt tctagattgt ttttctctga ttgagaatac gccctatatc ctttactttg
     5461 aatatatttt gaaatccaat cagctaattc ttctactttc tcttcttttt ccaagaactc
     5521 atcactatct gtaggtaagt tattgtatat atctgtaata aatttttttg ataatcctat
     5581 acaaaataaa attgctttag aaaaaccttg cgtttgattt gttggaaatt cagatatatc
     5641 aacaaaacga acaatatcta cacctttact gtataaaata gattttaatt catcattcat
     5701 atttgactcc tctccctaga taaaaataac attatacatt agtaaaattt attattttaa
     5761 ttatgttaac caatactttg tacattcttt atttctactc ataaaaccat actcaatcaa
     5821 atatcttcta attgttgcaa aatcatcata tatagattct aaaatctgat taacttcctt
     5881 ttctgaatat ttcttcccac gttcaaattg ttccaaaatc ttagataaaa ttacgacctt
     5941 tttcttttct tttgaagaaa atacttttaa ttttaaagga ttcatacttt caaatgatgt
     6001 atccaaaatg tgttttctct cttcttcagt tactacatat ctttcatcta ccattcgagc
     6061 atgttcatgg attggtatta tagtgctact tttattagat ttatcttcaa atacagattc
     6121 atatagtgct aaatataact tagcttgttt tgctttttct ctgaagataa atttttgatg
     6181 gcgtatagta gatgtggata ctccaatctg ttttgctatc tccttatctg gtaaatcact
     6241 aataaataat tgaaataatt gtttttgatt ttcagttaat gtgttgtact ttgaatcact
     6301 attaagcaat tgctctaaat aatcactatg ttctaattcc atatgaagtt caatagcttt
     6361 tgaagcttca aagaatctat tacctatttt atatatttct cctacctcat atgtttttgt
     6421 acatttaatg caactataac aatttaaatc agcattaaat atatagcctc gtttaatatc
     6481 atcaatagaa agattttcaa ttttcatgtt actacctcca tttcatagtt tacatttttc
     6541 ataaatacat tatactaaag tttattaata taatcaacat ttttgctttt tgtttataaa
     6601 acctaattat ttattaattt aaaattacat aatataaaaa tatatttaaa caaaactatt
     6661 caaattttta gc
//
LOCUS       DAEMAE010000199         6644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEMAE010000199 DAEMAE010000000
VERSION     DAEMAE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.201"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(536..1288)
                     /locus_tag="KPH93_003059"
     CDS             complement(536..1288)
                     /locus_tag="KPH93_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG1689833.1"
                     /translation="MFAIIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIAGSILGVRCVLSISEDILNVLV
                     VVMILIVAIYTFVSKNLGQEDNFESINKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1349..3004)
                     /locus_tag="KPH93_003060"
     CDS             complement(1349..3004)
                     /locus_tag="KPH93_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBG1689834.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKFDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDSKYKEI
                     FEPKKENKFCDICKSIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDVDKNISELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKIRKENIRLEEALKKANSKLLEEEYIINPIDMFN
                     NNLKEYPFELELYIEKEIKNMNLEGVCNLIDTNKLIYSDVRDIIQEMIFVLSRAVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIQKYYKENISLNSISEVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDI
                     SFSISDICKKVGFNNSSYFSQIFKKFNNVTPNEYRKNILDNKH"
     gene            complement(3233..3601)
                     /locus_tag="KPH93_003061"
     CDS             complement(3233..3601)
                     /locus_tag="KPH93_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1689835.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEG
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELEN
                     CMDKIDEKIYYLKEKKMCEK"
     gene            3750..4550
                     /locus_tag="KPH93_003062"
     CDS             3750..4550
                     /locus_tag="KPH93_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBG1689836.1"
                     /translation="MEKYATMIKEKLNEGKILCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHGALDRKDIQQHMIAAHAGGAASLVRLVNNDPDLVKPIMDMGTDIICFPFINTVQ
                     DAQKAVAACTYPPNGIRGYNPQRASYYGDMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVKGIDIIALGPGDLSLDMGFSGYMDRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAKILPLSLTLSINYYLFIMRLYQFKNNIKL"
     gene            complement(4698..5126)
                     /locus_tag="KPH93_003063"
     CDS             complement(4698..5126)
                     /locus_tag="KPH93_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG1689837.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPDEEAKDECIELGKMLAE"
     gene            complement(5611..6549)
                     /locus_tag="KPH93_003064"
     CDS             complement(5611..6549)
                     /locus_tag="KPH93_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG1689838.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVIQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWARDEFERDLDEAEL
                     YAQLIKCTRALGMPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2241 a         1099 c          666 g         2638 t
ORIGIN      
        1 taaaaaaatt aaattagcat aaccttgtta aatatagatt tgaatgtgaa tttaaagtta
       61 tgtaaaatta aaaatacagg aatgaaaatt ccattcctgt ataaaatcta acttttgagt
      121 ctcagtacaa tcttattttg agacaatctt ttttgtttta tattttctta tatttaaaat
      181 ataacgcata tcttatgcaa catattcata tttttcttta tattctttca tcgcatcatt
      241 ccaagcgtct tgtactgtta cacctctttt attagcaatt ttttgtgcca gttcaattat
      301 cttcatagct cttcttgata tgttcataaa cattcctcct acaaattacc atatatttca
      361 tatatatatc aaggttatat ttttatgata tataatcctt ctattgtaag attacaattt
      421 atatgtgtat tatatatgaa ctatattaga agataattag aatttttata aagctatatg
      481 attagattaa atataaaagg tttacaatta cttataaatt taaaagggct ataagctaaa
      541 ctaatgtttg atataccagt tttacgacca taaacaatgc cattactaaa aatattggtt
      601 ttatcatctt tgaacctttc tttatagcaa ctttagcacc tacataagct cccattatca
      661 tcactaatgc aaacactata gcaattttat aatctacttg accatttatc atgaataata
      721 acaatgaagt tatattactt gttaagttta atatttttgt atttgcagaa gcatgtaaaa
      781 aatcatatcc atatattttt ataaatccaa aagtcaaaaa agttcctgta cctggaccaa
      841 aaaaaccatc ataaaaaccc attataagag ccataagcat acctaatctt aaatttttct
      901 tattaataga ttcaaaatta tcttcttgac ccaaattttt agaaacaaag gtgtaaatag
      961 ccactattaa aatcataact acaaccaaaa catttaatat atcttcactt attgataata
     1021 cacatctcac acctaaaata ctaccagcaa tagtaaatgg tactaatttt tttaaaaggt
     1081 tattattgat ttttccagac tttgcgtatc tataagcact acttatacat cctgctgatg
     1141 ctgcaaattt attcgtgcca atagcaagat gtactggcac tccaatagcc ataagaacag
     1201 gcatacttat aagtcctcct cctcctgcta tagaatctac aaaagcagct aaaaaccctc
     1261 ctatacataa aaaaataatt gcaaacataa aaatcctcca tcattctctt tcaatttttc
     1321 ttacaataca tatgttatat gcttttattt aatgtttatt atctagtata ttctttctat
     1381 attcatttgg agttacatta ttaaatttct taaatatctg tgaaaaataa ctactattat
     1441 taaaacctac tttcttacaa atatcactaa tactaaaact tatatctaac aaatactttt
     1501 tagattcttc tatcctaaga agatttatat actcagttat tgaaacctta tttctttcat
     1561 taaaaataga actcaaataa ttggggctta taaaaaagac ttcagatata ctatttaaac
     1621 ttatattttc tttataatat ttttgtatat attcgtttat attttcaatt agatttcctt
     1681 tatcttttct ccaaaatgct ctattcttat caatacactc cttggaaaat tcaaataata
     1741 cattccataa atcttcatca ctgtctacaa gtgatatttt tttattatat ttatttctta
     1801 aataagatat aagttttaaa tcttctaaat ctctaagtac tgctctggat aaaacaaata
     1861 tcatttcctg aatgatatct ctaacatcac tatatattaa tttatttgta tctattagat
     1921 tacaaactcc ttctaaattc atatttttaa tttctttttc aatataaagt tctaattcaa
     1981 atggatattc ttttaaatta ttattgaaca tatctattgg attaataatg tactcttctt
     2041 ctaataattt actatttgct tttttaagtg cttcttcaag ccttatattt tcttttctaa
     2101 ttttcaatat ttcctcatct tttatactaa gttgattttc aaagaaattc ctcttagcta
     2161 tttcctgaat aaattcagac atcattaata aattttcttt taatacatat aatctacttt
     2221 taggatttaa tggaaattca ttgtaaattt tgtttatgta ttttatatca ataccggaac
     2281 tacttgataa ttcagatata tttttatcta catcaataga cttatatact ataaaattac
     2341 cacattctat atatccaact atattttctt ctaatactat aggtatctct aaactggaaa
     2401 caccatactc acaatggtac actttttcaa aattatcatt atttatgctt ttaacaagtt
     2461 ttttagtaca cggttttata cttttacaaa tatcgcaaaa tttattttct ttcttaggct
     2521 caaatatttc tttatacttt gagtcataaa cagatattct aagcttcaat atattagata
     2581 tttgatttgc aagattttca attatgttta cattaactag ctcttgtata ttgttctcag
     2641 aaaatactat gggtatttgt ctcaaactcg aaaatttatg aggaacataa gtaattaata
     2701 ttactaaatc aaatttacca gtgtttataa tttcatgttc tgaatatggt gggcaataaa
     2761 ctatagatgt ttcctttata ttgacctttt taccatcaat tatttgtatt ccttctccct
     2821 gaatcacata taaaagctgt tcttcactta aatgaaaatg cttttcttgg cagtttcctg
     2881 gaaaaaatct tacaattcca gcactaagtc tattgaattg tgtttttgat ggttcgtgta
     2941 tccacataac ctctccccaa tcaaattttt gtacttcatt atttgactca ataaaatcca
     3001 ccataaactc acctcgtagt ttttgtctaa atattttttt attcctttaa atatctcctt
     3061 ataatataca ttatcataat tttctttttt attgtataga taaatagtta tatagaacat
     3121 tgtccaattc ttcctaaaaa tatgttgtaa aaaatctttc ctcaaatgtt tccaattgaa
     3181 tttactcttt atattagaaa aatcaaaaaa ttatttcttt attctataat gattattttt
     3241 cacacatctt tttttctttt aagtaatata ttttctcatc tattttatcc atacaatttt
     3301 ctagttcatg tattttattt ttaatatttt gcttatgttg ttgaaataat tggattcttt
     3361 tatctattgt accatctccc atatcacaaa aatgaaagca ttcatggata tctttaatag
     3421 ataaaccagt attttttaat tctttgataa atattagacg ctctacatct ccttcagaga
     3481 aatagcgaac accacttggt tttctattga taggaagtaa taaacctttt tcttcataaa
     3541 atcgaatagc agatgaagaa atttttgtta tttttgatac atcagttata gaatatttca
     3601 taatctttac ctccctaaat tttatatgaa taagaatatt ataaaaatac cttatacagt
     3661 agtcaacaaa aaagtattga cttcaagtta ggttgaagtt gtactctttg aagtgtaaaa
     3721 gaaaacttac aaaagaaagg atgatataaa tggaaaaata tgcaactatg attaaagaaa
     3781 agctaaatga aggaaagatc ttatgtggag tagctgtttc actgtctgat tcggttgtaa
     3841 gtgagttaat cggtatatct ggatatgatt ttatttggat tgaaggagaa catggtgctt
     3901 tagaccgtaa agacattcag caacatatga tagctgcaca tgctggtggc gctgcttctt
     3961 tggtacgatt agtaaacaat gaccctgacc ttgttaagcc cataatggat atgggaacag
     4021 atattatttg tttcccattt attaatacag tacaagatgc acaaaaagcc gtagcagctt
     4081 gtacttatcc tccaaacggt ataagaggat acaatcctca aagagcctct tattatgggg
     4141 atatgaatta ttttgactat gtcaaaaacg caaacaatga aacattaaaa atgatgttaa
     4201 ttgaacagaa agcaggttat gataatcttg aagaaatagt aaaagtcaag ggtattgata
     4261 ttatagcatt aggccctggt gacttatcct tagacatggg attttccgga tatatggata
     4321 gaattgaaat aaaagatatg atttctcatg ctgctgaaat atgcaaaaaa tataagatgc
     4381 cctttattat ttttcctaca atagaaaaga aaagtattga agaatgggtt aatattggag
     4441 caaatatgct ttgttttgcc aagatactac ctttatcact tacgttatca atcaattatt
     4501 atctttttat aatgagattg taccaattca aaaacaacat taaattataa atctaatttt
     4561 atactaaaag agactaatat tttatctatc tctattaagg atattttctt atgtatcata
     4621 atattttagt ctctcttagt attatatact gctatcatca ttagttgcta tttatgctat
     4681 ttttataata tacaaattta ttcagctaac attttgccta attctataca ctcatctttt
     4741 gcttcttcat ctgggtcttc cattgctgta attccttctc caccaataat tgtggcacca
     4801 gcattttgca tacgttcttc ccaatctctc atccattcac aatttcccca tccatatgaa
     4861 ccaaataatc caatctgttt acctgatacc atagattcta attctgaaac aaatggctcc
     4921 atatacccct cttctagttg ctctgctccc atagatgggc aacctaatgc aaatttatct
     4981 tcttctttta aatcacttgg ttttagtaag ctaacatcta aaacttctgg tgtcttacct
     5041 tttaacttca caccttcagc tacaaaattt gccatttttt cagtatttcc tgttccactc
     5101 caataaacaa tatatatttt actcattttt acctcctgta gtatttttta attaatcaat
     5161 agttagttat agctaactaa catctaaaaa acaaaaaatt cctttatctg aaatctcttt
     5221 atttttctaa atatagaata tcataattga ttttgaaatt caatatttat tgataatata
     5281 tatcatttat attttatatt tcttatatat ctcaaaacat tgtatcgtat ttctaatctt
     5341 tcatagaaaa atttctaact tatagtactt ttgcatctct tataaatcat cttatatatc
     5401 ttttatatgt ataagtacat agaaaaacac acctttccca gatgtgctat ctataatatc
     5461 gtatatttat ttttcaattc tgactagcta atatgtttat aaattttatt acttaatacc
     5521 tttatacatt gatgtaaaat cttacaccta agtaattttt tatttgtctc aaccctatat
     5581 aacatagcta ttttatcatc aaatcaaaaa ttatcttttg ctattattct tgtatttata
     5641 aagatttaaa tataagtatc catttccttg ttctgttcta ggcattccaa gtgctctcgt
     5701 acattttatt aattgtgcat acagttcagc ttcatctaaa tctctttcaa actcatctct
     5761 tgcccattct ttaattagtg ctagtgaccc tgatacgtat ggtgcactca tactagtacc
     5821 actcataata gcaagtttat tatccatata tgttgatata atatttcttc ctggagctac
     5881 caaatctatt gtagtatttg aattactaaa cttttcaacc aaatagtttt catttattgc
     5941 acctacttct attacctcag cataactggc tggataacta tactcacttg tactagaatc
     6001 accatctcca ttattacctg ctgcacacac tacagaaata ttatttttta ctgcttgtat
     6061 gacagcattt tttaaattct tatcatcttt gtttcctcca agagacatag atataatatc
     6121 aaccttgtta tttacagcaa agttaatagc attaattata ctttgatatg ttccagtacc
     6181 atctttattt aatgcttttg ctattaataa tttacaatct ggagctactc ccataacttc
     6241 attattataa ttagatgcag ctataatacc cgccacatga gttccatgac cattaaaatc
     6301 ctcatatata tttttatttc cattactgtc atcactaaaa tttgcaccac caattatttt
     6361 tccttttaaa agaggatggg atatatcaca acctgtatct attataccaa ctacaatatt
     6421 tttaccagta taaccttcat cccacattcc cctagcattc atttgtttta ttccatatgg
     6481 gaatttattt tttgcacctc ttagattctc atttatttca tacggaatta gtttcattct
     6541 tttattcatc atatatcctc ctaaaaattt tattgcttat aaaaatcata aattttattt
     6601 tataataggt ttataaatca attcaaaacc tatatctgct tatt
//
LOCUS       DAEMAE010000200         6531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEMAE010000200 DAEMAE010000000
VERSION     DAEMAE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.202"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            244..1077
                     /locus_tag="KPH93_003065"
     CDS             244..1077
                     /locus_tag="KPH93_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG1689839.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIAVGVRVSSLNTKENEIS
                     RSAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1170..2102
                     /locus_tag="KPH93_003066"
     CDS             1170..2102
                     /locus_tag="KPH93_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1689840.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFSKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2154..2636
                     /locus_tag="KPH93_003067"
     CDS             2154..2636
                     /locus_tag="KPH93_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1689841.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLVDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2648..3517
                     /locus_tag="KPH93_003068"
     CDS             2648..3517
                     /locus_tag="KPH93_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG1689842.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKIAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIQGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKIHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKAINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3551..4276
                     /locus_tag="KPH93_003069"
     CDS             3551..4276
                     /locus_tag="KPH93_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG1689843.1"
                     /translation="MFKIAIVSICLLVSLVDINPIFAGSSSNETSNSDTIKVLNAKSL
                     NFKQGNYEVKVSIPKIKGLKDKTLENKLNNEFLQYGEKIYSEFQNKMKKENNKGHRYL
                     NVIYNVKNNSKDFLSIEMIKEEIEASSSVTKKHYTVDKNREILLNLPIFFKDSRSIRV
                     ISDNIKEQMRNQIKKDDTKSYFIDQDKDSSMKDFESINKYQDFYFNKDGNLVISFDEY
                     EVAPGYMGAVEFVIADKVIKRLR"
     gene            4318..5139
                     /locus_tag="KPH93_003070"
     CDS             4318..5139
                     /locus_tag="KPH93_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG1689844.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPDSEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRENGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5144..5905
                     /locus_tag="KPH93_003071"
     CDS             5144..5905
                     /locus_tag="KPH93_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG1689845.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYKIIVQTNDTSTDFIKKSLKSWYKMESERVVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTSQKNIYINSKISMARLDVIDYVIVHEFSHLV
                     HMNHSKKFYDLVKTIMPDYKDKENWLKINGYKIII"
     misc_binding    6065..6322
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2880 a          615 c         1104 g         1932 t
ORIGIN      
        1 aaattataaa tgacatttta actagaatta attgtaaaaa acactatata atactcaatt
       61 ttcaacattc tatacaatta tacagaaatt atacagcata ataatcaccg aaataacaaa
      121 ttttaaaaat taagcaaaac tttttgtcga agttcaacgt ctatatatat gtagtgtaaa
      181 tttttgggat aaaatactac aaaaatataa atgtaaaaat atagtaatat tggaggtcga
      241 acgatgagaa ataaaaagaa aaaaataaat agaaagaagt tgtatttgct tttaagtgct
      301 attgtagtat gtctcgcttt tatagcagtt ggtgttcgcg ttagctcttt aaatacgaag
      361 gaaaatgaaa ttagtagaag tgcaaaactg tcaagttcta ctattacaga tatagtatct
      421 aatacatcaa tagaagttag taaagggcca agtaaaagtt ctggaaaaat agcatacata
      481 actatagatg atggtccatc taaatttact gaccagatga taaaaacttt aaataaatac
      541 aatgttaaag caactttttt tatgatagat gggaatatga aggaatatcc acagcaagtt
      601 aaaaatataa ttaaaaatgg gaacacagca ggttttcata gtgtatcaca tgatattcac
      661 aagctttatg ttactagtac atcagcaaaa gaggaatttg atacaaatga tcaaactttt
      721 tataagataa ctggtaaaca ttcaaaagta ataagaatac cttatggaag caaaccatat
      781 acaccacaag cctcttatca ggctttagtt gatgctggat ataaaatttg ggactgggac
      841 ttagatacag aagactggag atctaattct tctcaaattg tacaaaatgt aaaaaatcat
      901 attaagaata gaaaaggtga agataaagac caacttgtag ttttaatgca tgagaaaaaa
      961 cagagtgcag aagctttaga ttcagtttta aaattcttat cagatgaagg ttatgaattt
     1021 gcgccagtag atcaaaacca aataccaaaa aattattggt tacgtaattt agagtagtta
     1081 aaatataaga atattgattg atatttttag aaattgagaa taaaaatgag tgaggtgtag
     1141 gtatattgaa taaaaaactt tatataggaa tggtcggaat attgtctttg atgatggtcg
     1201 gttgtaataa aagtttagca aaagtaaatg atgtggaaat cacaaaagaa cagtacaaaa
     1261 aaacaaaagc agtcttatct gctactaata attatataaa tgggcagtct ttagatgaat
     1321 tagaaaaaac tttggataaa aaaggtagaa ataaactaga aaatgttata atatcgttta
     1381 tggtggataa tgaactttta tatcaagagg ctaaggataa aggactaact ccaagtaaaa
     1441 gtgaagtaga ctcaaaatat caagaactag aagataagat gaatttgaat acaagctaca
     1501 aggaaaaaat ggacaaagca ggtgttgata aagaatattt aaaacaagaa atatctagag
     1561 atttagcaat agataaaaat aaaaaggcct ttgaagatag aataaacata agtgataatg
     1621 atatggaagc ctactataca agtcataaaa aagattttaa tgtagaagaa gtaagtgctt
     1681 ctcagatact tatatcgact ttagataaaa ataaaaaaga agtaagcaaa gataaaaaag
     1741 aagctttgaa gaagaaggca gataatatat tgactaaaat caaaaatgga gaaagttttg
     1801 aaagtttggc taaaaaatat tcggatgata aagcaactgg caaaaatgga ggacagctag
     1861 gatatttttc aaaggatgat aaaaatgctg agtttacaaa agaagtgttc aaattaaaga
     1921 aaaatgaagt ttcaaatgtg tttgaaacta gttatggata tcatatagtc aaagttactg
     1981 ataaaagaga aagacaaaaa agttttaatg agtgtcagag tttaataaga gaatctattt
     2041 taaatgaaaa gtatattgag catataaaaa aattgaatga agatgctaaa atagacagat
     2101 agaatttcta acagctcaat cctagcctgt aatcggaggg agattttaca ggaatgttta
     2161 agagtttaaa gagaacgaaa gttgagaaat acattatcga taataaagac tctttttata
     2221 gaatagcata tagttatacg aagaatgaag aagatgcctt agatgtggtt caagaagcca
     2281 tgtataaggc actatactca gtggaaaata ttaaagaagt aaattatata aagacatggt
     2341 tttataaaat tttagttaga acgagtatag attttattag aaaaaaccgt aaatataata
     2401 atatgacaga tattgacctt gtagatgaga ctggagagta tgataaatat actgatttgg
     2461 atttaagaag agctttggaa gaattaccta ttgaatacaa atctattata atattaagat
     2521 tttttgaaga tttaaagatt gaagaagtag caataatatt ggatgaaaat gtgaatactg
     2581 ttaaaactag gttatatact gctttaaaaa aactaaaatt aaaaattgaa gagtagggat
     2641 aaaaagaatg acaagcaaag aaaggttaaa taagttaaaa gatgagtatc ataaaattcc
     2701 aatacccaag aaattagata ctataataaa tcatgaaaaa atagaaaaca tatatcgaga
     2761 gaaaaataaa aaagtaaata gattaagatt taaaattgca atagcatttg catgtatatt
     2821 tacagtatta gtaaatataa gtcctgtatt tgcagataat ttttctaaga tacctgtaat
     2881 tggagcaata gtagaagtaa taactataaa aaattatagt ttaaaaagtg aaaattatga
     2941 agctgagatt gatattccta aaatacaagg tttaaaagat aaaaacttag aacaacgatt
     3001 aaacagtagt tttatggaag atggaaaaag actttatcat caattccaag agagaatgga
     3061 aaagatacaa tcatcaaaga ataaaggata taaatcttta agtttgagtt atagtgtaaa
     3121 aaataattct aaaaaatttc tatcaataga gatgacaaaa aatgagatag aggcttcatc
     3181 atatgtgagt aagatacatt atactattga taaaaaaaga caaatagttt tgactttacc
     3241 aatgcttttt aaagatgata agtatataaa agttataagt gataatataa aagaacaaat
     3301 gagagaacaa atgaaaaagg atagtacaaa atcctatttt atagatcaaa aaaaagactt
     3361 gcctgttgaa gattttaaag caattaataa gtatcaggat ttttatttta ataaaaatga
     3421 agaccttgta atttgctttg acgaatatga agtagcacct ggttatatgg gtgcagttga
     3481 gtttgtaata ccatataaag taataaaaga tttgtaaatt gaatagtagt ttatatataa
     3541 ataaaaatct atatttaaaa ttgctatagt ttcaatatgc ttattagttt cactagtaga
     3601 tataaaccct atatttgcag gtagttcctc taatgagact tcaaacagtg atacaataaa
     3661 ggtattaaat gctaagagtc tcaactttaa acaaggcaat tatgaagtga aggttagtat
     3721 tcctaaaata aaaggtttaa aagataaaac tttagagaat aaattaaaca atgagttttt
     3781 gcaatatggg gaaaaaattt acagtgaatt tcaaaataaa atgaaaaagg aaaataataa
     3841 ggggcataga tacctaaatg taatctataa tgtcaaaaat aattctaaag attttttatc
     3901 aatagaaatg ataaaagaag agatagaagc ttcatcatct gtaactaaga agcattacac
     3961 tgttgataaa aatagagaaa tactattaaa tttgccaata ttttttaaag atagtagatc
     4021 tattagagtt ataagtgata atataaaaga gcagatgaga aatcaaatta agaaagatga
     4081 cacaaaatct tattttatag accaagataa agattcctca atgaaagatt ttgaatctat
     4141 aaataagtat caagattttt attttaataa agatggaaat cttgtaatta gttttgatga
     4201 atatgaagta gcacctggtt atatgggagc agtagaattt gtgatagcag ataaagtaat
     4261 aaaaagacta agatagatat atagaagatt tttagataga ctggtaagga gaattatatg
     4321 aatatatatg aacaagcaat aagattaata gaaaataatg aggattttgc atttgctaca
     4381 ataacatctc attcaggttc aacaccacgt gaaactggag caatgatgat tgtgaaaaat
     4441 gattctacta tatttggctc agtaggagga ggtagtgtag aagcagaatg tataaaacat
     4501 gcaattaatg ttataaaaaa tagagaatca atgttgtata aatttactct aaacaaatct
     4561 gatgtggcaa aactaggaat gatatgtgga ggtactggag aaatacaaat agattttata
     4621 gatagtaaat taaaaagtaa tatagaaaaa tttaataaga gactaaagga aaatacaagt
     4681 aaagcgtata tatttggggc aggtcatatt tcaagagatg tcgctgtgat actatctctt
     4741 ttagaattta gaacagtagt aatagatgat agagaagaat ttgctaatca tgaaaggttt
     4801 ccagactcag aagtagtagt tttagattct tttgaaaata taccagattt tccaactgac
     4861 gaaaatagct atataattat tttaacaaga ggtcatctat atgattcaag tgctttagaa
     4921 tgggctctta aaagagagaa tggttatata ggtatgattg gaagtagaac aaaaattgga
     4981 ttgacgtacg aaaaactcat gaaaaaaggt tttaagaagg aagaattatc aaaagtacat
     5041 gcaccaatag gcataaaatt gaatgctcaa acaccagctg aaatagctgt atgtatagct
     5101 gctgaactta taaattgtag agctaataag gaaaaataaa ccaatgaaca cagggagtaa
     5161 agatattgat actttaaatg ttaattttag agggattgaa ataaaagcag ttttagtata
     5221 taaaaataga aaaaatattt ctataaaaat agatccattt ggcaaaataa ttgtaatgag
     5281 tcctcctaaa atatctaaaa aaataataaa agatataatt attgaaaaag gagactggat
     5341 acttaaaaag tcagaaaaat ataaaggaag agaagaagta tacaaacaaa gaatgtttat
     5401 aactggcgaa aaatttcttt atttaggtga agagtatcat ctagttataa aagaactgtt
     5461 gaaagattct gtcaaaaaaa gtaactgtag aataactatt aacgaatata aaataattgt
     5521 tcagacaaat gatacaagta cagattttat taaaaaatct ttaaaatctt ggtataagat
     5581 ggaaagtgaa agagtagtat tagagagaat agattttctt aaattaaatt gtgaaataat
     5641 gaagcaatta gttccagcaa gtgttaaggt aaaagaacaa aataagagat ggggaagttg
     5701 tacatctcaa aaaaatattt atataaactc aaaaatatct atggcaagat tagatgtaat
     5761 tgattatgta atagtacatg agtttagtca tttagtacat atgaatcact ctaaaaaatt
     5821 ctacgattta gtaaaaacca ttatgcccga ttataaagat aaagaaaatt ggttaaaaat
     5881 aaatggatat aaaataataa tctgaaaacg attattccaa aatataggtg taaagtataa
     5941 aaaaagtatt attgtgaaaa aaataaataa tctgaaaatt ttaaaaaagc cattgacatt
     6001 gtaaatgata gggaatataa tctatattaa caacaaaaca gaattaaaat ataaaattaa
     6061 aaaattaaaa gcaatgacag agacaacaat actaatagtg ttatccacag agaaccaatg
     6121 ttgctgagaa ttggtgttaa cagtagtata atgatcactc tggagctttt gtactgaata
     6181 tttttagtaa gtacaaacgt ctgtcaacgt tatatgacta aggttaaggt aattaattac
     6241 ccgacctgaa aaaagagggg tagatgtatt ttttatatct acaaataagg tggtaccgcg
     6301 gaatataact ttcgtcctta taagtaatat ttatattgct tataaagatg aaagtttttt
     6361 tattttaagc gatataaata aatttaatcc atataaaatt aataaaaaaa taataaaaat
     6421 aaggaggaat ttttatggaa aatactgtat atgccaaatt aaaccaagga atagattctt
     6481 ttttaagagt ggctatgact ctaagaagaa gagaagttga tatacaatca a
//
LOCUS       DAEMAE010000201         6439 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEMAE010000201 DAEMAE010000000
VERSION     DAEMAE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.203"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(235..2622)
                     /gene="cadA"
                     /locus_tag="KPH93_003072"
     CDS             complement(235..2622)
                     /gene="cadA"
                     /locus_tag="KPH93_003072"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG1689846.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKISIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEADDVINKIKEIINSTEPGLD
                     IQVESTNKVKGRTTEKPGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGISMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2645..3010)
                     /locus_tag="KPH93_003073"
     CDS             complement(2645..3010)
                     /locus_tag="KPH93_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689847.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3210..4061
                     /locus_tag="KPH93_003074"
     CDS             3210..4061
                     /locus_tag="KPH93_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689848.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMNSNMGMPNNMNSNMGMPNNMPNNMNSNMGMPNNMNS
                     NMGIPNNMSNNMNSNMGMPNNMSGNMNNMYGNTSMPNNMNSNMGYNMNNTMGIPNFSC
                     NQNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQTTNIE
                     EFDEEEM"
     gene            complement(4498..5586)
                     /locus_tag="KPH93_003075"
     CDS             complement(4498..5586)
                     /locus_tag="KPH93_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689849.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5711..6439
                     /locus_tag="KPH93_003076"
     CDS             5711..6439
                     /locus_tag="KPH93_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG1689850.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2258 a         1072 c          702 g         2407 t
ORIGIN      
        1 aacttattaa taaatcttct atatctaaat tttaaaatta caatataaaa gctctacact
       61 aaataaatac attaaataaa cataatgtat aatgaatata gatttatata tacaaaaacg
      121 ctccaataaa aaggtaccaa taaagttaaa agaaacttat tgataccttt tattattata
      181 tacttcaata tataattata agttttttga atagtatgga tttacattta tcccttatct
      241 catagctctc atagcattta ctactgctat aagtgccaca cctacatcag caaatatagc
      301 ttcccacata gtagctactc ctccagcacc tagtatcata actataattt ttactcctag
      361 agcaaatatt atgttttgcc acactatctt attagtcttg tttgctattt caattgcttt
      421 agatatcttg ctaggctcat ctgtcataag cactacatct gcagcttcaa tagcagcatc
      481 tgaacctaag cctcccatag ctataccaac atcagcacga gctaatactg gagcatcatt
      541 tattccatca cctacaaatg ctattttctc tttttcagtt cttccttcat ataaatcttc
      601 taatctatct accttttcat taggaagtaa atttgagtaa actgtatcca attctaattc
      661 ttgagcaata ttttttgcta ctttctcatt atctccagtt aacattacaa cttcttttac
      721 ccctattgct tttaagcttc ttatggcatt tttactatct tcttttactt catcagatat
      781 aactatataa cctctatata ctttatcaac agcaatatag actactgtac ctacttcttt
      841 agctgatgaa taagatatat tttctttttt cataagtttc tcattacctg ctaatataaa
      901 attgccatta tgctttactc taattccata agctgctatt tcctcataac tgtctattgt
      961 atctaaatca atttttttat tatagtaact taatatagac tttgctattg gatgatttga
     1021 atttacctcc gcaatagctg catattctaa taattcttct tctgatatac cttcaggatt
     1081 taacttagtt acattgaaca cacctttagt aagtgttcct gttttatcaa atactactgt
     1141 atctacactt cttaaagctt ctaaatagtt acttccttta ataaggatac catttttaga
     1201 tgcaaaccca attccactaa agaaactaag tggtattgat aaaactaatg cacaagggca
     1261 agaaacgact aagaatatca atcctctata taaccaatca ctaaatacct cacctgagat
     1321 tacaagtggt ggcacaaatg ctattaataa agcagctatt actactattg gagtatagta
     1381 tcttgaaaac ttagatataa agttttctgt ctttgacttt ttaatgctag aattttcaac
     1441 aaggtcaaga atcttagaaa ctgttgattc tccaaaactc tttgtaactt caatactcaa
     1501 taaagcattt ttatttataa cacctgataa aatttcgtct cctttttcaa cttctctcag
     1561 tactgactct cctgtaagtg cagaagtatc tagcatagaa attccatcca ctactacacc
     1621 atctaatggt actttctcac caggttttac tactattata tcgcctacat ttacttcttc
     1681 tggtgacacc accttaactt cagaattaac ttttaaattt gcatagtctg gtcttatttg
     1741 cataagtgaa gttatagact ttcttgattt accaactgca acaccttgta aatactctcc
     1801 tactttatag aaaagcataa cccctactgc ttctgataac tcccctattg ctaatgctcc
     1861 tactgttgct agagccatca aaaagttctc atcaaatact cttccttttg atatatttct
     1921 tattgctctt aaaagcacat cgccacctac tattacatag gcaactataa atactatatt
     1981 gctaaactga ctttcataac ctgtagctgt ttgatatata ccaaatatat atacaagtgc
     2041 tccaattata agtggaatta attctttttt atttgtatca tttacagctc ctggtttttc
     2101 agtagttctc ccttttactt tgttagtaga ctctacttgt atatcaagcc ctggctctgt
     2161 tgaatttatt atttctttta ttttatttat aacatcatcc gcttcaaagt tattacttat
     2221 atttactgta agttttttat ttacaaagtt taaattagaa gattcaactt cttttaattt
     2281 agaaactttg ttattaattt cctctgcaca atgtgcacag tttaatcctc ctaatataag
     2341 ctctttttta attgatattt tactagatgc attttcttta gaacttattt gtatatcaag
     2401 ccctggttct gttgaatcta ttatatctat tatcttttct atagtagtat cctcattaaa
     2461 gctttccttt atatttactg taagtttttt atttataaaa tttaaattag aagatttaac
     2521 ttcttgtagt ttagaaactt tattattaat ctcttctgca cagtgagcac aatttaatcc
     2581 tcctaggata aattcttttt taattaaatt atttgcttcc atatatattc actcctctca
     2641 tttatcattt ataagtctct ctaatatgat ttaatccaca atcaaatatc tgacttatat
     2701 gactatcatc taatgagtaa tacactgttt ttccttctct tctaaacttt actaatctag
     2761 cctgctttaa tactctaagt tgatgagata ttgcagagtg agtcatattt aataaactcg
     2821 ctatatcaca tacacacatt tcatttgcaa ataatgcata taatatcttt actctagtag
     2881 tatccccaaa tactttaaat aattctgcta aatcatataa catctcttca tctggcatag
     2941 ttgatttagc ctctgttact atttcttcat gtactatatt acaattacaa tcatttatct
     3001 cttctctcat ttttaatcct ccaattattt taatatatga ataactgttc atatgttcat
     3061 tatatgctat catttctaaa tttgcaacaa taatttttat taatttaata taattagaat
     3121 gcacatatta taaacttttt taatatatat attattagaa taagtttttg taaacttttt
     3181 tatagttttc taggaggtgc aagtttaata tgagtaacaa gaaaaaaaag gatttagata
     3241 ctagttacat gccttactta gacccagcaa actatgcaga ttatattact aatgaagaaa
     3301 ttccccaaga tgaattgaga aatgaccctc aaatttctaa tggttataca aaaataccta
     3361 ataaccagaa cataaataac ctatatccta ataatgtaaa tcattatcct aaaagtagta
     3421 attctccaaa caacttgcct aacacaaata atacatccgg aaatatgaat tacaatacat
     3481 ctgctacaaa taatatgcct aataacatga ataataatac gggtatgcct aataatatgt
     3541 ctaacaatat gaatagtaat atgggtatgc ctaacaacat gaatagtaat atgggtatgc
     3601 ctaacaacat gaatagtaac atgggtatgc ctaacaatat gcctaataat atgaatagta
     3661 acatgggtat gcctaataac atgaatagta atatgggtat acctaacaat atgtctaata
     3721 atatgaatag taatatgggt atgcctaaca atatgtctgg taatatgaat aatatgtatg
     3781 gtaatacaag tatgcctaat aacatgaaca gtaatatggg gtataatatg aacaatacta
     3841 tgggtattcc taacttctca tgtaatcaaa atatgcctcc taatgtactt atgatgatac
     3901 caggagttat atgtcataat acaatgcaag gtatgcctgt agttatgcct agtactatgc
     3961 ctcctaatat atatcctact ccttatggtt ctagtaatat gtctatacaa ggtataccac
     4021 agactactaa tattgaagaa tttgatgagg aagaaatgta atatatattt ttttaagtaa
     4081 gttgtataaa acattaataa ttgaccataa taataaatgt attcctcata atattccccc
     4141 aatattatta taggtataga aagttgactg aatatgcgct tattcggtca acagatatta
     4201 acataatcta ataagataaa catagaaaaa gtcagataaa atttaatttt atctgacttt
     4261 ttcatttaaa aatgctctct tgtgttggta ggtcacaata gtatggtact cgtatttgag
     4321 actaatattg gcaaacaaaa taaagaaaag tataaatttt ttataataaa ctgtataaaa
     4381 ttttaataat tgaccacaat aataaatgta ttcctcataa tattccccca atattataga
     4441 aataaattta agcgagatta aattcagtta atctcgctta aataatgtct aaatttatta
     4501 ttccaaaaaa tcatcaaaat ctaatgaacc tgattctgca aagtcatata atgcattttg
     4561 ttcaaagaaa aatttctctt caaatttaat atagtcacta taattagatt gacttatttc
     4621 attgatataa ttgcaatcat tttcgttata agtaccaata aaaaacttat ttcccattaa
     4681 gtaatatata gctttcatat cattatttat tatatataca ttttctttta catggtcatt
     4741 attaaaatta aatctagtaa aaaatatacc ttcattcata ttacttttta acaaaaatgg
     4801 attttctcta taaaatactc ctgcaaattc ttcaggtgaa tttacactgt atacacttac
     4861 aaattcactt ttagaaatct catcaaatac ctcaaaatca tacataggtt ctttatctgt
     4921 cactaataga gagttaatcc taaaaccagt ttcataacac ttacttatgc aaaaagcaat
     4981 tttacatata tagtatccat cattatcttc atataaagct tcacttatat atctgccttg
     5041 acctttatca cttaccacat ttttaagtaa tgttccattt atattagtaa tatgcatatt
     5101 tgcaatctcg tcacttccag aaaagtactt taaactttct ctatcccatg ctataaatct
     5161 catagtcata taatttataa attcaacttc ttcatcaact cttttacata ttttattgta
     5221 taatttttct atagttaaat ctgttttata gttttttata atatcaatat actcacattt
     5281 atctcctggc aatagtttat cataataaag attcttatta ccaaaataag atactaaaaa
     5341 taaagattca tccttcgtta attcaactct atctgaacca aatcctccca tcaatctttc
     5401 ttcttccata taagcttctt cttgtgttgg gttatttaaa ctcacataat ctgctaatcc
     5461 taatccctct gcatctaata aaaaatattg acaaaagtga ttgttttcat catctatcca
     5521 atttattata agacctaaac ttcccataag tctagaattt gtagcctttg caaaattaaa
     5581 attcatatag tcacatcctt taaactctaa aatacttgtt ttttaatata taaattatta
     5641 tacatatatt ttaacaactt agatacaatt tataatataa tataaggaaa tgattaattg
     5701 aggtggtctt atgaaaaata tagcagcttt ttttgatata gatggtactc tttatagaga
     5761 ctctcttatg gttgaacatt ttaagaaatt aattaaatac gatataattg accaaaaaaa
     5821 ttggtataaa catgctagag acacttttat ggattgggat aaaagacaag gtaattatga
     5881 tgattatcta ttagaaatat gtgaccttta tgttgattca ttaaaaggat tagataaaac
     5941 ctgtatcaac tttacaagtg accaagtaat taaattaaaa gcagatagag tctataaata
     6001 cacacgttca aggattgagt ggcatctaaa tcaaggacac atagttatat ttatatctgg
     6061 tagccctgga tttcttgttg aaaaaatggc aaaaaaatat aatgtaaccg attttctagg
     6121 aagtgattat gtctttgaaa ataatatatt tacaggcaca gttattccaa tgtgggattc
     6181 tataagtaag aataatgcta taaacgattt tgttgtaaag tatgatttag atttatcaaa
     6241 ctcgtatgcc tatggagata ctaatggtga tataaatatg ctaaaaagag ttggaaatcc
     6301 aatagcaata aatccaacaa aggaactttt atctcaaata tcacttgatg aggaaatttc
     6361 taaaaatgtt gagataatag tagaaagaaa agatattatt tattctctat ctacagatgt
     6421 aaatatagtg gatatttaa
//
LOCUS       DAEMAE010000202         6418 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEMAE010000202 DAEMAE010000000
VERSION     DAEMAE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.204"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..931
                     /locus_tag="KPH93_003077"
     CDS             <1..931
                     /locus_tag="KPH93_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1689851.1"
                     /translation="KKNHKIINPFSVKISQQYPTVFNLSIQFSKIIENEYHIKISQDE
                     IGFIATHIAVPFEKREEANFNKKYKIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSL
                     LEEKAVIEFAPDLIFSITNLLFEISAPVILINEILDELDYLDIKESVRFADHIGNISS
                     PKQYILGLFDKNHFRCIKEITKYKDILDSMSQCIVDEGACSQTYPKDVWERESYLSTI
                     YTNGVAIPHPIEMTGNKNIISVALIQTDISYENRVPKIIFMISLIKGNLELHKQISKY
                     LSKIMADKDMVDMLNKSQSYEEFMYKLKIYLGG"
     gene            934..1977
                     /gene="ypdE"
                     /locus_tag="KPH93_003078"
     CDS             934..1977
                     /gene="ypdE"
                     /locus_tag="KPH93_003078"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG1689852.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKVICDGLGS
                     IIFSKIKDESAPNIMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFTQKIRITT
                     REGKKIPAIINATYNNGKAENIYADIGAYTEEDVYSLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELKKENINCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDRGVMLMHRDRTLVPSRKMIDYVMKIADENNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLKNIIKLYTQLILN
                     FDEEKYKHIRNFI"
     gene            1992..2297
                     /locus_tag="KPH93_003079"
     CDS             1992..2297
                     /locus_tag="KPH93_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1689853.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDVEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            2394..2729
                     /locus_tag="KPH93_003080"
     CDS             2394..2729
                     /locus_tag="KPH93_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1689854.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            2761..4083
                     /locus_tag="KPH93_003081"
     CDS             2761..4083
                     /locus_tag="KPH93_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1689855.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFVSLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQREETKNIFSKEEEDLLDDLGLDF"
     gene            4093..5163
                     /locus_tag="KPH93_003082"
     CDS             4093..5163
                     /locus_tag="KPH93_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1689856.1"
                     /translation="MNNTMERLVSLICKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQIIDGRYIDEADKKTTGFIKRVVSQGSYIPDIIELLRELHIKKLAIENQAMSI
                     QEYLSLKNAGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFSEVVCEIKKDMT
                     ELEVSALIQYHALRKGASGMAFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGVNARDIDKVAREIIDKY
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILTEGMVMSCEPGIYIPNVGGVRVEDDI
                     VIINGKGVSLNKTSKEFIILGE"
     gene            5168..6355
                     /locus_tag="KPH93_003083"
     CDS             5168..6355
                     /locus_tag="KPH93_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1689857.1"
                     /translation="MKYNFDKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAIVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDLKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDSMTSYVEENFEIVYDFFETHIPKANVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLKEALNRIKKTYIG
                     MYC"
BASE COUNT         2437 a          716 c         1238 g         2027 t
ORIGIN      
        1 aaagaagaat cacaagataa taaatccttt ttcagttaaa ataagtcaac agtatccaac
       61 tgtttttaat ttatctattc aattttcaaa aattatagaa aatgagtatc atataaaaat
      121 aagtcaagat gaaataggat ttatagctac tcacattgct gttccctttg aaaagagaga
      181 agaggctaat tttaataaaa aatataaaat agcaataata tgttcttctg gcggaggcag
      241 tgcatttttg ataaatttaa gacttaaaga aatctttcca aacgctgaga taagaaattt
      301 ttcattgcta gaagaaaaag ctgtaataga atttgcacca gacttaattt tttcaataac
      361 aaatctttta tttgaaataa gcgctccagt tatacttata aatgaaattt tagacgaact
      421 ggattattta gatatcaaag aaagtgttag atttgcagac catataggaa atatatcaag
      481 cccaaaacaa tatatattag gtctgtttga taagaaccac tttaggtgta taaaagagat
      541 aaccaagtat aaggatatcc ttgatagtat gtcacagtgt atagttgatg agggagcttg
      601 ttctcaaact tatcccaaag atgtgtggga aagagaaagt tatttaagta ctatttatac
      661 aaatggggtg gcgatacctc atccaattga aatgacaggt aataaaaata ttatatcagt
      721 agctttaata caaactgata ttagttatga aaatagggtg ccaaagatta tttttatgat
      781 ttcattaata aagggaaatc tagagcttca taaacaaata tctaaatatt taagtaagat
      841 tatggcagat aaggatatgg tagatatgct aaataagtca caatcttatg aagagtttat
      901 gtataagtta aaaatctatc tgggaggtta gacatgaata tagagttttt taagaggtta
      961 tcagaagcag atggcatagc atctaatgaa gaagaggtta gacatgtttt attagaggag
     1021 ctaaaagctt atagtgataa agttatatgt gatgggctag ggagtataat cttttctaaa
     1081 ataaaagatg aaagtgcacc aaatataatg atttgtgctc atatggatga agttggattt
     1141 atggtgagaa gtatagataa acttggtatg attcacttaa taacaatagg aggagtaaaa
     1201 cctcttgcac agtttacaca aaaaattaga attacaacaa gagaagggaa aaaaatccct
     1261 gctatcataa atgctacata taataatggt aaggctgaaa atatatatgc agatattgga
     1321 gcatatacag aagaagatgt atatagttta ggcattaacg ttggggatat ggtaacatat
     1381 acaacaagct ttgaggaatt tactttgcca gaccgattag tgggaaaagc ttttgatgat
     1441 aggataggat gttttgtgat gggagaagtt ttaaaagagc ttaagaaaga aaatataaat
     1501 tgtaatattc actttgcagc tacaagtagt gaagaagttg gaataagggg ggcaaagaca
     1561 tcaacacaat taataaatcc cgatatagta tttgtaatag atgtagcatg tgctaagaat
     1621 gagtttgtga gagattatac aaatcagaaa caaattgata gaggtgtaat gctaatgcat
     1681 agagatagga ctttagtccc tagtagaaaa atgatagatt atgtaatgaa aattgctgat
     1741 gaaaataata taccacttca acatgatatg tttgaatcag gtgggactga tggaggagaa
     1801 gcacatcttg taaatgaagg gaaaccttgt gtagtaactt gtgtaccagt tagatatggt
     1861 cattgtgcat tttcaatagt aagtaataaa gatttaaaga atattataaa attgtatacg
     1921 cagttaattt taaattttga tgaagaaaaa tataaacata taagaaattt tatctaaagg
     1981 agaataagaa aatgtcaaca gaagtacaaa taatggagct tatatcaact gcaggagaga
     2041 gtaaatcgaa agcttttgag gctttaaaaa aagtaaaatc aaaagatttt aaaggagcaa
     2101 aattgttaat agaggaggct cgtaaaatag atgttgaggc tcacagaata cagacagaat
     2161 taataactca agatttaaat gaagatgttg aaagcccagc aatgagtctt ttaatggtac
     2221 atgctcaaga ccactatatg acttctcaac tagcgaaaga cttgatagag gctttgatat
     2281 attgtcttga taagtagttg tagtgtataa atagattgtt tatgtaaaaa ttcttttaag
     2341 agtttagata cttttaagag ataataaaaa tacaaacttt aggaggaatt attatgaaaa
     2401 tattattatg ttgtgcaggt ggattttcta ctaatatgtt aatgcaaaat atgaaaaagg
     2461 ttgtaaaaga gagtgctaaa cttaatgaaa aagattttga gtttacagct ataccagcag
     2521 attcaataga aagtgaagtt gatgattggg atatagtact tataggtcct cagatagctc
     2581 acaaaataga cttcttaaaa ggaatattag agccaaaaaa taaacctttt gcagtaatag
     2641 ataaagatgt gtatgggaaa atggatgggg ctacagtcat gaagttggca ttaataactt
     2701 ataagaaaca tcaattagct caaggataat aataataatg agggaaaagg gggattagat
     2761 atgttttcaa aatttgaagc atttatgaat agagtattta tgcctttagc tcacaaggtt
     2821 gacaatcaac gtcaccttgg ttctataaag gctggtatgg ttgctatgac gccattcaca
     2881 atattaggaa gtatatttgc aatacttcca gcacttccaa atatgcttgg agaaaataat
     2941 ccaataagca aattcatact gaataattca gcaatattag acttaccagt aagtctttcc
     3001 attggcttga taggattata tgcttgtatg tgtattgctt ttaatctggg taactactat
     3061 aaattatata taccaggttg tgttgcacta tcaacttttt cttttttatt cctagtagct
     3121 tcatttacac cagatggagc tttagaaata ggaaatttag gagctaaagg gctatttaca
     3181 ggtatgatag cagggcttgg ttctgtggag ttatataatt tttgtaataa gaagaatatc
     3241 acaataaaaa tgccagaaaa tgtacctgac tttgtgtcta ggtcttttga attaatacct
     3301 accactataa tagtttgtgg aacttttata gttgctcgtt ttgtttcatt aaaggtattt
     3361 ggagaattac ctccacagat acttactcaa tttttatctc cattggtagg aagtatggat
     3421 aacccatggt cagttcttgc acttaacttt gctatatgca ctatattctt ttttggaata
     3481 cattcatcag tatttagccc tataactaga cctataatga ttacatttat agctgaaaac
     3541 atagcagcta tgcaagctgg ggaaacaata cctcatttct ttacagcagg agcttcaagt
     3601 gcgttctttg gatttacagg ttgtggaatt agtataggtt gtgtaattgc gtgtatgtta
     3661 tcaaaaaatg catcttataa gaagattgga cgtgtgtcat tattccctgc attatttggg
     3721 ataaatgaac caatattatt tggagcgccc ataatattaa atccaataat gtttatacca
     3781 catgtatttg gtggagctat aataggtaca ttaccaatgt tttttatgca ttggggatgg
     3841 ttagcaaaac cagtatttga ccctccatat gtgggagtgt tcttagaagg atttttaact
     3901 aatggagatt ggcattctat attagcaaat gcattacaat tagtattagc agttttatta
     3961 tattggccat tctttaaaat aatggaacgt caacaaaata ataaaaaaca aagagaagaa
     4021 actaagaata tattctctaa agaagaagaa gacttattgg atgatttagg gttggatttc
     4081 taatatttta ttatgaataa tacaatggag agattagtat ccttaatctg taaaagaggt
     4141 gttgacgctc ttttgattaa agcaaaaaat actaaaagat acataggagc attatcaggt
     4201 agtggtgtat acgtacttgt tactaaagat aaaaactatc aaattataga tggaagatat
     4261 attgatgaag cagataaaaa aacgactgga ttcataaaaa gggttgtatc tcaaggttct
     4321 tatataccag atataattga gttattaaga gaattgcata taaaaaagtt agcaatagaa
     4381 aaccaagcta tgagtataca agagtatctt tcactaaaaa atgcaggttt tgaaatcaat
     4441 ttaataacca acgaattatg gaagctaagg gctataaaat ctaaagaaga aattgaactt
     4501 attaaaaagg cttgtgagat tacagatgaa gtatttagtg aggttgtttg tgaaattaaa
     4561 aaagatatga ctgagctaga agtatcagct ctaattcagt atcatgcatt gagaaaaggt
     4621 gcaagtggaa tggcttttga aacaatcgtg gtatcaggag aaagaggtgc aatgccacat
     4681 ggaagaccta caaataaaaa attgaaagta aatgaagcta taacgataga ttttggagtt
     4741 gtttatcaag gatatcaatc ggatatgact cgtacaatat ctataggtaa acctcctaag
     4801 attattaaag agatatatga tgtagtatta gaagcacaat tatctgcaat agagtcaata
     4861 aaggaaggcg ttaatgctcg tgatattgac aaagtggcta gagaaattat agataaatat
     4921 ggatttggtg aatattttaa tcatggattg ggtcatggta taggtcttgg agatggcgaa
     4981 gttcccactc taaatccaaa tagtgaagat atattaacag agggaatggt aatgtcttgt
     5041 gagccaggta tttatattcc aaatgtaggg ggagttagag tagaagatga cattgttata
     5101 ataaatggta aaggtgtttc acttaataag acatctaaag agtttataat attaggagag
     5161 taaaattatg aaatataatt ttgataaagt attaaatcgc aaggaaggaa attgtaggaa
     5221 atggagcaac catgtaatta aagaaaagtt cggtttaaat gaagatgcaa ttcctatgga
     5281 tttagctgat atagattttg agtgtgctcc tgctataaaa gaatctatct taaaaagagc
     5341 atcagtagga gattatagtt atacttttat aggagatgac ttctatgatg ctattataaa
     5401 ttggaataaa agaagatttg atgtgtatat agaaaaagac tggataaaat taacttttgg
     5461 tactgtttca actcttcatt atatagtcca agcatatact aaagaaggag agggtgtatt
     5521 gataaataca cctgcttatg acccttttgc agaggctata gtaaataata atagaaagtt
     5581 gtgttgtagt tcattaaagt tagataacaa taggtattat ttagatttag aagatatgga
     5641 aaatcaaatg aaatacgaga atataagatt gcttatattc tgtagtccac aaaatccatc
     5701 aggtcgtata tggacaaaag aagaactata tcaagtgtca gagttatgtt taaagtataa
     5761 tgtaattttg gtaagtgatg aaattcatag agatataata tttaaagatt atagatttgt
     5821 atctttatgg aatgctcatc cagaaattta taaaaatagc ataatttgtg tatctcctaa
     5881 taaagggttc aacttaggtg gattaaaaac gtcatatgta ttaataagag atttaaaaat
     5941 tagggagact ttattagaaa gactaaaaag caattcaata acttctccaa atgtatttgc
     6001 aataccagca atcgttgcag cgtacaatga aagcgaagaa tggctagatt ctatgactag
     6061 ttatgtagag gagaattttg aaatagttta tgatttcttt gaaactcata taccaaaagc
     6121 caatgtgatg aagtcagact cttctttttt agcctggata gatgtaagag aagtgtttag
     6181 aaatgaagaa gaatctaaag aattttttag acatgcaaac ttaactatgg tagttggaag
     6241 ttattttgtg aaagatggag atggatttat tcgtattaat gtgggatgcc ctagagaaac
     6301 attaaaggaa gctctaaata gaatcaaaaa aacgtatata ggtatgtatt gttaaattat
     6361 aaaaaaggta tctatggttt atataaaaac acattattta gttatcaaat attactgg
//
LOCUS       DAEMAE010000203         6337 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEMAE010000203 DAEMAE010000000
VERSION     DAEMAE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.205"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(54..662)
                     /locus_tag="KPH93_003084"
     CDS             complement(54..662)
                     /locus_tag="KPH93_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG1689858.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFIAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKYDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDIYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            complement(888..1592)
                     /locus_tag="KPH93_003085"
     CDS             complement(888..1592)
                     /locus_tag="KPH93_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1689859.1"
                     /translation="MFNIELENKEYKLLCKNYSKNDGVKAIFLYLVIMLVLFLQGYLY
                     KTNLSPTILNVLQGIFSTLILFIGLGFVLFSKEKLNTIGITRKNLKQSLFYGILGATI
                     LIIFIILFNKVIEGKKVVFLFPTITTAITFIVTAISEEVVFRGYIQTRLTGLIQNNII
                     TSCINAFLFLSTHYPVRWAASGFSVTILSGFYVVCLILLHFVCDLVYRKTNCIWGSYL
                     LHVLYNVFTSILIFSI"
     gene            complement(1987..2532)
                     /locus_tag="KPH93_003086"
     CDS             complement(1987..2532)
                     /locus_tag="KPH93_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG1689860.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(2650..4473)
                     /locus_tag="KPH93_003087"
     CDS             complement(2650..4473)
                     /locus_tag="KPH93_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689861.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSIILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVDNVFKSELNDNKDGSLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSIDRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANSHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVEYGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(4486..6261)
                     /locus_tag="KPH93_003088"
     CDS             complement(4486..6261)
                     /locus_tag="KPH93_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689862.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLNYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIERDESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNISFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVIKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         2168 a         1082 c          568 g         2519 t
ORIGIN      
        1 aaaaattttt tcagattaat taaatctttt tatatatttt ataataatcc aaatcatgct
       61 aaacatttag acagaaaact tttttcttta atagtaaata cttttgtttg aatgtcagaa
      121 taattatttg aatatatatc tataaaacca tgatttgcat ctaaaatctc aatttggata
      181 ttttgtttcc tagaaagttg gtaagaaata gaatcaacat caaatgaatc gtactttgca
      241 aatactaata aggtaggaca ttttggaatt acatccatgt aatctcgaat ccgagaacca
      301 taacaacaaa ttatgccatc acaaaaagaa tcttcactgc atctccatgc aatagttgct
      361 cccacgctat acccagcaat aaatacatat ttatactctg attttaaatc atttataagc
      421 ttttcaactt ttttataaat atcaaaacca acattattta taaaaaaatc atatgcctct
      481 tggacttttg aatatggaaa aactcttttt tctcccagaa gttcaggaca aaatacatca
      541 tatccatatt tataatactt ttgacaaaca tcttctataa agctattaat cccatagatt
      601 tcatgtaaaa caacaatagc tacatcactt ttatgtatat actttatcaa attatccacc
      661 acctttacaa aaaaataaat ttttatattt aatttaatgt attcctctaa atatatcaat
      721 tttctgtttt aaaacttaat ttatttctta attcaatatt atttactatg cattcctcaa
      781 attaaaaaga tatatatcct taatcagata cacgtctata attatactag ttaatttaca
      841 aaattagcta gacttttaca atcaattgct tatcaaattg gaattagtta aatagaaaaa
      901 atcaaaattg atgtaaagac attatatagt acatgtagta ggtatgaacc ccatatacaa
      961 ttagtttttc tatatactaa atcacacaca aagtgaagaa gtataagaca tactacataa
     1021 aatccagata aaattgtaac tgaaaagcca gatgctgccc atcttacagg atagtgtgta
     1081 gatagaaaaa gaaaagcatt tatacatgat gtaattatat tgttttgtat caatccagtt
     1141 aatctcgttt gaatgtatcc tctaaaaaca acttcttctg aaattgcagt aacaataaat
     1201 gtaatagctg tagtaatagt aggaaataaa aaaacaactt tttttccttc tataacttta
     1261 ttgaataaaa taatgaaaat tatgagtata gtagcaccta aaattccata aaaaagactt
     1321 tgctttaaat tttttcttgt aattccaatt gtatttaatt tttcttttga aaataataca
     1381 aaccccaacc ctataaataa aattaaggtt gaaaatattc cttgcagcac atttaaaatt
     1441 gttggactta aatttgtttt atataaatag ccttgtaaaa acaataccaa cataattact
     1501 aaataaagaa atatagcttt tactccatca tttttagagt agttcttaca taatagctta
     1561 tattccttat tttcaagttc aatattaaac attaatgtta ttacctccta gtcaaagtta
     1621 tctttattta acttttataa tgtatcatac ttatactaca tacgtcaaat tttgactcat
     1681 acattattca tattttgata tatctactct attcattagt tttgatgcaa aagtatgcat
     1741 taactttttt acgattatta tacctatttc acatagtaga aaatctctat aaacccaata
     1801 atcacaatgt ttttctatct actagttttt gtttactgct tcctagttca tctcaattaa
     1861 ttttgtaaaa atctgttcaa atattagtca tttttagcta aaatttaata actaaaaaag
     1921 agtaccttac ttataatttt aaaaattata aaataagata ctcctaaaat ctactttata
     1981 ttaaatttat tttccaaaat atctatttaa taatcctaag aatgctttac catgtctagc
     2041 ttcatcttta cacatttcat gtactgtatc atgtatagca tctaatccta attcttttgc
     2101 tttcttagct aattttaatt ttccatcagt tgcaccatac tcagcatcaa ctctaactct
     2161 taagttttct tttgtatctg caactacaac ttctccaaga agttctgcaa atttagcagc
     2221 atgttcagct tcttcaagag ctattctctt ataagcttct cctacttctg gataaccttc
     2281 tctatcagct tgtctactca ttgctaaata cattcctact tctgtacact caccaacaaa
     2341 gttagctctt aatccttcga ttatttcttc atctacacct tgagctactc ctattctgtg
     2401 ctcatcagcc caattttttt cgcctttcat ttcttcaaat ttatcagctc caactttaca
     2461 tactggacat tgtgctggtg cagcatctcc ttcatgtata tatccacata ctgtacaaac
     2521 aaatttcttc ataattactt tcctcctaaa ttttatatat caaattaaat tttttattta
     2581 ttaagttagt ctatatatac ccgcaccata tacctttaaa caattttttt taaagttttt
     2641 tctaaatact tattttccct gtgtcaataa tatgtttcta taataatgac cttttaattt
     2701 tatgagttct ttatgatttc catattccac aattctacca ttttccaaaa ctattatatt
     2761 atcacaattt gatatggtac ttaatctatg agctataatt atacttgtct ttccataagt
     2821 taaattattt attgcttctt gaattttagc ttctgttgct atatctatat tacttgtagc
     2881 ctcatccaaa ataagtattg gagcattttt taatactgct ctagcaatac ttattaattg
     2941 tttttgacct tcacttaaat cttcacccct atctgataat acagtactat atccttcacg
     3001 cattttaact ataaatttat gtgaatttgc tattttacta gcatatataa cttcttcatc
     3061 agtagcctcc agatttccat accttatatt ttccataact gtatcattga atatataacc
     3121 ttcttgtgga actactccaa aacaagttct caaatagttt ctgtcaatag aatatatatc
     3181 cttaccatct aaaaatatac ttccttcatc tatatgataa aaatttgtaa ttagatttat
     3241 aattgttgtt tttccacttc ccgtctctcc tatgatggca tttgatgtgc cagctttgat
     3301 ttttaggttt atattgtcta aaattttctt ttttgaatca taagaaaaac ttacatcttt
     3361 aagctcaatt tctcccttta caaaatgaga gtttaaactc ccatctttat tatcgttaag
     3421 ctcactttta aacacgttat ctacttcttg ctcttcttgt tcatctataa tttcaaaaac
     3481 tctttcacat ccagctatgg ctgaaagtaa tgtattaaat aaatttgcca attcatttaa
     3541 aggtcttgtg aattgtctta cataacttaa aaaactagct ataaccccta tggttataat
     3601 atctttagat gcaagaacgc ctcctataat acatatggtt gcaaatccta tattactaat
     3661 cacattcata ataggcatta aaagagaagt gtaaatttgt gatttaaggg aaatatttag
     3721 aagtctatca ttgacttctt taaaatcatt tacagatttt ctttcataat taaaagattt
     3781 tactacattt atgtttgata tcatttcttc aatatgtgca tttagttttc ccaactcaac
     3841 ttgttgttgc ttaaaaaaaa ctctagttct ctttgcaata aaattactca caaacattac
     3901 tatcggtatt aatattatac tgcaaaaggt taaaattggg cttaatacaa acatcataat
     3961 aaaagtcatc aatatgttta agattcctgt aattaactgt actaatgaat ttgatattcc
     4021 tgtactaata ttatcaacat catttgttac tctgctcata atatctccag tagaactttt
     4081 atcaaaaaag gttattggta gatttttaaa ttttaaaaat aaattacttc tcatctcttt
     4141 aactatactt tgagataatc tagctgtaca tatcccctta gacagtttaa ctgaaaactc
     4201 aattatataa aatgaaagta aaataattat tccttttaag aaaatggatt ttgaataagt
     4261 atcgatatct attatgtcaa ctacctttcc aatagtatat ggtattacag ttgatataat
     4321 tgaatcaatt attactagca aaaaaataaa tctagtaatc ttcttatctt ttttaaagta
     4381 cataaaaatt cttttaaacg tttttttaaa atctttaggt ttttcttttt tctttaatct
     4441 actaaccttt tgatttccag tacttaaatt caatatactt cctccttaat ttacatcttc
     4501 acttactaaa ttctgcaacg caaaaatttt tttataaatt ggagaactaa ccagtaaatc
     4561 ttcatgtgta gcaaatccaa ccacctctcc ctcatccaag actaaaatct tatcacagtc
     4621 aattatagaa ctaatctttt gtgaaatagt tattacagta catttcttac tttccttttc
     4681 tcttaaatta cttatatatt cttttatatt ctttcttata attgtctctg tattaatatc
     4741 cattgcacta aaacaatcat caagaattaa tatttgtggt ttttttgcta tagctctagc
     4801 tatagtaatt ctttgtcttt gtcctcctga tagattctta cccctttgaa ataaaacctc
     4861 atcataagta ttttcgagtt ttgttataaa ttgatttgca tttgctatct cgcatgattt
     4921 tataacatcc tctttgcata tatctctatt tcccataact acattctcca atatgctttt
     4981 tccaaacaca ctactatttt gaaatactac acctattctt tcccttaaac tttcttcaga
     5041 aatactgtat atactacgtc ctccaatctt aatatcgcct ttatcaatta tatataattt
     5101 ttgaattaaa tttactaaag aagtctttcc tgaaccagtt tgaccaatca caccaatatt
     5161 ttgaccttct tctattttaa aggatatgtt ttttaatatt ttttcatctg tatttttgtt
     5221 atacgaaaaa tctacatttt caaaaattat atcacaattt acaaacttta aatctttttc
     5281 tccaacatct aaactttcat ctctctcaat tacttcttca ttctcatcaa ccaaaacctc
     5341 acttattctc tcataagacg cttttgccct aacaaaaaca ttaaaaacat gtgaaatcat
     5401 tataagagat gttaatattc tagtcatata gtttacataa gcaactataa tcccaatttt
     5461 aatttctcct gaataaatta aatgccttcc aatcagtaat attattatta tagaaacatt
     5521 aatgattatt gtaattgtcg gtctaaaaaa tgacatcata gttgtagctt ttctactatt
     5581 tttaaaaagt tcatcattta actcttcaaa actttggttc tcataatcgt atctacaaaa
     5641 tagtttaact acttttattc cacttaaaaa ctgtcttagt tttgaattta ctttgtctgt
     5701 actttcttgt acatttttaa ataatctata ccctatcttc atattgagca atattataat
     5761 aaatataatt aaaactgcaa ttagaataat tattgacatc tttaaattta attttatagc
     5821 tataattaaa cttcctatac acattattgg tgactttata aaaactctca tcattccatt
     5881 aacgaattgt tgcatttgat tgatatcatt agtaattctt gttattaatg aagctctccc
     5941 aaatttatct atttgtttta atgagtatgt atttatcttt tcaaatacat cctgtcttaa
     6001 atcgtaagaa aaatgctgag aaactcttgc tgagattaca cttcttatta ttgcaaatat
     6061 tgcacctaaa aaagttatac ctatcattat agctcctgtt gatagtatgt aatttaagtt
     6121 cttatttgaa acccctatat ctattatttt tgccattata gttggttgag ctaaatcaca
     6181 aaaagcttcc aatgttaaaa aaaatactgc tgttagaaat aatactatat atttttttat
     6241 gtatttttta aaatacctca ttcaatcatc ccttcataga attttctcat taccaaaagt
     6301 atatcataat ggctaattta attgattata tcaatta
//
LOCUS       DAEMAE010000204         6298 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEMAE010000204 DAEMAE010000000
VERSION     DAEMAE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.206"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(102..1337)
                     /locus_tag="KPH93_003089"
     CDS             complement(102..1337)
                     /locus_tag="KPH93_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689863.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DDNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDVYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAIAAPTF
                     SGEYQTQSDSILRCTKHGHIRLAFTKILALFSIFIIMFVICISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(1338..2225)
                     /locus_tag="KPH93_003090"
     CDS             complement(1338..2225)
                     /locus_tag="KPH93_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689864.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYTMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(2370..3485)
                     /locus_tag="KPH93_003091"
     CDS             complement(2370..3485)
                     /locus_tag="KPH93_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689865.1"
                     /translation="MKKINNFTYRKLRDKYEFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPDMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(3521..4219)
                     /gene="vanR"
                     /locus_tag="KPH93_003092"
     CDS             complement(3521..4219)
                     /gene="vanR"
                     /locus_tag="KPH93_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG1689866.1"
                     /translation="MNGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQLRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(4600..5280)
                     /locus_tag="KPH93_003093"
     CDS             complement(4600..5280)
                     /locus_tag="KPH93_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689867.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(5330..>6298)
                     /locus_tag="KPH93_003094"
     CDS             complement(5330..>6298)
                     /locus_tag="KPH93_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689868.1"
                     /translation="SSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAITSNRFEPI
                     FTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNERK
                     KFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQE
                     FTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLTR
                     AFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTERD
                     NNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKETVFTIKLSLCFL"
BASE COUNT         2122 a         1048 c          709 g         2419 t
ORIGIN      
        1 ctatatctgc tattaagatt tgttaactaa atttaacttt tatactacat atataattat
       61 tgtatcctct agtttatatc actattattt ccatcaaatt gttacgatac ttgatgtttg
      121 caataggtac gaatagccaa aaatataaaa ataggtattt ctatcaccga gaaaaacagt
      181 ataatgtgtg gtgtccaaaa actcatacct cctaagtgta agaaattaaa tgatgtcatt
      241 tgatataata aactaatctt aaaaccaatt ccccctggcg gtaataggta tccaatccaa
      301 ctaacaccta ttcccatagg aattaagcaa atagaaaaag caatcagcat agaagtaaga
      361 gaatccttac attttgcaga taaaaataat gttaaactaa ccattgcaag tattgaaatt
      421 agaccaccaa tagctataag aatctgtgct tgtagtaaat ttatatttac tagactaatg
      481 acagaaaaca acatttgaaa tgaggttttc aaacattcag taccaaaagc taaatcagaa
      541 attgccaaat ggatagaaat acatataaca aacattatta taaatataga aaatagagct
      601 aatattttag taaacgctag acgaatatgt ccatgtttcg tacaacgaag gatgctatct
      661 gattgtgtct gatactcacc agaaaaagta ggtgcagcaa ttgcaacaca tataattaat
      721 aatataaaaa tataaaatat tatataatcg aaagcatctt tagaataacc tgggtatatc
      781 tcaaatggag tatctacttt tctataaagt tcttgagctt tcatttgtgc actggaagtt
      841 tgtcgttcaa tcttcataat atcatttaaa tgagttttgc attgttcata aaaagaatct
      901 aaatcatttg tatttatttc tgataattct gttgcaaatc ctgtttctga attagcaaat
      961 gcttcaggaa gtctattcaa caatgggcga attggtaaaa tgcttttaga ataaacatct
     1021 agtggaaaat cttcactacc tactttacca tacttttctt ctgttttttt ccatatattt
     1081 aaagcatgct ttaatttttt tggtgttaca actccactac taatttcact attattagca
     1141 taaattgttt ttttatattt aatagcatct aaaccactca attcaactac cttgccatta
     1201 tcatctaaat aattgatacc ctcataagta attggaagaa aagccatcaa tgcagataaa
     1261 accaacgcca acaataataa aattaatgta cgacgtgagt gcaacactcg tttaatttcc
     1321 aaataaaaaa tacgcattta tttcccctcc ttactataaa ctgcttctgg aaataaccat
     1381 aaatataaat cttctaatcg tggttcaaca gcaatagagt tatcaatttg tgacttttct
     1441 gccaagtaac gaatagatac ttgattgttt ttttcactac gctgattgat tatacgtaaa
     1501 ttcatttcat attttgaaat atctctattt gatataagac attcccatac ttttccctct
     1561 actttcttaa ccagtttatc ggttgttccc acatcaagaa tttttccact tttcataata
     1621 gcattttgag ttgatatgta ctcaatatca gatacaatgt gagtagatat caggacaaca
     1681 cgattatgag aaaattctga aataaaatta cgaaatctca ctcgctcacc agggtcaagc
     1741 cctgctgttg gttcatccat tataaggatt ttagggtcat ttaacatggc ttgtgcaatt
     1801 cccactcgtc gtttcattcc accagataat ttaataatct ttttattttt tacatcagat
     1861 aatgtaagga tttccaatag attattaatt tttcctttta cttctcttgg tggtacatct
     1921 ttcaatgctg ctatatattc taaataatct tttacagaaa aatcctgtgg gaaaccaaat
     1981 tcttgtggta aaaatcccag taaatttctg tactcagcac ccattgtata aatatttttt
     2041 ccattataca gaattttgcc attggtaggt ttcataatgt ctgtaataat tctcataagg
     2101 gtagttttac ctgctccgtt cgctcctaga aatccccaca cacctggtgt taaagtaagg
     2161 caaacatctt ctacagcaat tttatcctta aattttttcg tcacatgttc aatttttaat
     2221 tccatatttg aaactcctct ctttatatac ttacctgcaa aaacaaaagt gccttgctca
     2281 gtatttattt tactaaacaa agacactcat agttttaata ttaaatagat aatttcttaa
     2341 gaaaatccta atatttgatt tatattttac taaatagatg taggaagcga tatagtaaat
     2401 gttgtgactt catctttact ttgaacagta atatcaccac catgcaaatt tataatttcc
     2461 ttagctatag aaagccctaa acctgcacca cccatatcag attttctagc ttcatctaaa
     2521 cgattgaact tttcaaatat agcagaaagt tgttcttccg gaattgtctg tccataattt
     2581 ctaaaagcaa tatctacctt gctgtcttta tgaaccgctg agataataat ttctgtatta
     2641 ggatagctgt aagcaatagc atttttcaag atattattaa atactcttgc aagtttttca
     2701 gaatctgctt ttattgttaa actttcattt gcttttagaa tggctctatt tcctttttca
     2761 gatagcaatg ggtaaaattc atcctttaac tgaagtaaca tataatatag gtctactttt
     2821 tcgaaattaa ttttgatttg attagaatta tatcgtgtta tttcaaaaag ttcattgata
     2881 agtttttcta aatattgtgc tttgtcaagt gcaacttcag tatacttaga tttttgagaa
     2941 ttatccatat caggtatttc ttttaataag ctcaaatacc caataactga agtaagtggt
     3001 gtccttatat catgtgctag atacatcaca aggttattct ttttattttc agcctctttc
     3061 actgcttgtt gacttaacaa aacagacatc ttaatttggt tcatttgttg ctctatttcc
     3121 tttaatggct tagatagttc aaccaattca tcattttgtt gataaatagt atgtgttgca
     3181 ttgatgattt ctcttaaata attccatggt tttttccaat aaaaattgaa aatagtgata
     3241 tatccaatta taaaaaccaa aaaaaataat acatctcttc tcaacttaag ccatttaaca
     3301 agaggatttg aaaaatttga aaacgtataa tcagccataa acattaaaac caatactata
     3361 cctgtataga tagctaacat tatatataat ttatacttta aatacgcctc tgtatttttt
     3421 tctcttttta taaattcata tttatctctt agtttcctat aagtaaaatt attaatcttt
     3481 ttcataatac tacatccttt cttgacaaga aaaataggtg tcaaatcttg taaccaacac
     3541 cccaaattgt ttttatatat tttgcttcat ctacagtatc acctaacttt tcacgaagat
     3601 ggcgaatatg aactgttata gtattgttac ttttattgta atattcatct ttccagatag
     3661 catgaaaaag attttctgaa tttaccacag agcctttatt ttttaataat acatgtagaa
     3721 tagaaaactc tgttggagta agtgaaagag ctttaccatt taaatgacat tcatatgttt
     3781 gggtattcaa ttccagacct aaaaaagaaa aaatatgtga aagttcttca ccagattgta
     3841 aacttccact gtacttctta tatcttctaa gctgtgcctt aactctagca acaagttcca
     3901 aaggtcggaa aggtttagta acataatcat ctgcacctaa agttaagcct gtaatcttgt
     3961 cggtttcttc atccttagct gttaacataa tgattggata agtatatttt tctcggattt
     4021 tttggcataa agaaaatcca tttactcctg gcaacataac atctaaaatt gcaaggtcaa
     4081 attcatttaa attaatgcag tttagtgcat caagaccaga ataaaattta taaacaatat
     4141 agttttcatt tgtaaggtag acttctatta aatcagctat atcacgttcg tcatctacaa
     4201 ctaaaatttt tccattcata gactttaaac atcctttcta tatatattta tatatattat
     4261 actaaattta atttttttta tataattttt tacattagaa tttattaaga aattcttaat
     4321 cttattattt aactatttta ttatttaata tatattaaag ataaaaaaat aatatataga
     4381 tttagatata caatttattt atcttttcaa atataatcta taaaataata cttccgttac
     4441 gtatttttta tataataaaa ctgactgtaa tttaactggc aaagtttaga ataataggct
     4501 ttatcaatta aattacagtc agctatttta taataattta gtttagatat cgttagcatt
     4561 tcacatttat aataattatt aatgatatta atatgtttat tattccttat taaaataata
     4621 tcctgctcct cttattgtta taattttctg aggattttta taatctttct ctattttctt
     4681 tcttaatcca ttcactgcaa ctactacact atagttatcc tctaagtatt cattcatcca
     4741 tacatgttca tatatctctt tagtagtcaa agtcttttca ggattctcta taaaaaagtt
     4801 taacaactta ttttctgttg gagtcaattt tattatttta tcttctaact ttattactcc
     4861 actagttcta ttatattcct ctaattttgt attattttca ttattattta catatgagtt
     4921 cattcttctt aaaatagctt ttacttttgc atataatatt tctaaattta gaggctttat
     4981 taagtaatca tctccaccag aatttaaagc atttaaaatg ctttggtctt catttatata
     5041 agtcatataa acaattggac aagtagttac atttctaatt tttttacaaa gagtccatcc
     5101 atcaccatca cttagaatta tttctaaaaa tattaaatca tatttattgc taaatatttt
     5161 tccaatagct tcttcacaat tatatgctaa atctatactt attccttttt cctccagaaa
     5221 tccttttagc ttctggcaat tatccttgtc tccatctatt atcaatatct tagtttgcat
     5281 tataatcact cccaaatatc attattcttt tatattaaat aagctattat tataaaaaac
     5341 atagacttaa ttttattgta aaaacagttt cttttcctag ttctgaatct acaaagattt
     5401 ctccaccatg ctctcttata atggatttga caatatataa ccctaaccct tgcccttttg
     5461 gctcattatt atctctttct gtaaagaatc tgtcaaatac ctttggtata ttttctggag
     5521 gtataccttt tccattatca gaaattttaa taattgcaaa tccatcttct ttatccattg
     5581 ataaaattat ctttccatca aatggagtaa aggtcaaagc attataaatt aaattctcaa
     5641 atgctcttgt aagcatctct ttatttatct ttgcatatat ttttttctcc aatacataag
     5701 tttcaaagtt tacaccacat gcttgtgtat ctggcaaatt attttcatat atctctttaa
     5761 taaaatcatt cagacatatt ttttcactaa taccaatgct tttatcttgt gatgtaaatt
     5821 cttgcaaact acgtactcta ttttgtactt catttgactt ttgatttata gcataaagat
     5881 aggttcttgt ctcttcatct acatgaatat taccaacctt tattaagtct ataaatgctt
     5941 gtatagccga aacaggtgcc tttaaatcat gtgctacatc tgccaagaat tttttacgtt
     6001 cattcaacaa tgttgtaagt tcattagttc tcacttcaac ttcatgttca agattccttg
     6061 taagtttttc attctctttt attattattc tattcctgtt tatcatcata acagaaaata
     6121 aaataacaat tacaaaccca caatattctg tttgccaccc tgtaaatata ggctcaaatc
     6181 tattactagt aattgcatca aataatatgc taattccaaa aacagtgttt actgaaacta
     6241 aaatataaga acttatatgc tcagagcatg tctttttata tattgaaacc agcgaaga
//
LOCUS       DAEMAE010000205         6291 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEMAE010000205 DAEMAE010000000
VERSION     DAEMAE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.207"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(13..681)
                     /locus_tag="KPH93_003095"
     CDS             complement(13..681)
                     /locus_tag="KPH93_003095"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG1689869.1"
                     /translation="MDNKCNNVYSDEAEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
     gene            complement(709..1467)
                     /gene="hisF"
                     /locus_tag="KPH93_003096"
     CDS             complement(709..1467)
                     /gene="hisF"
                     /locus_tag="KPH93_003096"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG1689870.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDSGA
                     DELVFYDITASNEDRKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEDSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKMEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            complement(1449..2171)
                     /gene="hisA"
                     /locus_tag="KPH93_003097"
     CDS             complement(1449..2171)
                     /gene="hisA"
                     /locus_tag="KPH93_003097"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG1689871.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAVENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFEIYEKIAKETSLNVIASGGVTSIEDIKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLEE"
     gene            complement(2168..2773)
                     /gene="hisH"
                     /locus_tag="KPH93_003098"
     CDS             complement(2168..2773)
                     /gene="hisH"
                     /locus_tag="KPH93_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG1689872.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDINEIKQADSLILP
                     GVGAFRDSISALDKLGLIPVIKEHVSKGKFIIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            complement(2775..3353)
                     /gene="hisB"
                     /locus_tag="KPH93_003099"
     CDS             complement(2775..3353)
                     /gene="hisB"
                     /locus_tag="KPH93_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG1689873.1"
                     /translation="MRTWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGVRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            complement(3356..4405)
                     /gene="hisC"
                     /locus_tag="KPH93_003100"
     CDS             complement(3356..4405)
                     /gene="hisC"
                     /locus_tag="KPH93_003100"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG1689874.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSNLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDSVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            complement(4439..5065)
                     /locus_tag="KPH93_003101"
     CDS             complement(4439..5065)
                     /locus_tag="KPH93_003101"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG1689875.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            complement(5087..6031)
                     /locus_tag="KPH93_003102"
     CDS             complement(5087..6031)
                     /locus_tag="KPH93_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG1689876.1"
                     /translation="MKFLRIEDEVIYFKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
BASE COUNT         1942 a         1242 c          534 g         2573 t
ORIGIN      
        1 cattaatcta ttctattttc ttcttttaag taattctttt tttatttcat caatttcaac
       61 acctttttct atcattaaaa ccaatgtatg atatactaaa tcgctaattt cgtaaataag
      121 ttcactttta tcggtatttt ttgatgctat aattacctca ctactttctt cacctacttt
      181 tttaagaatc ttatctattc ctttttcaaa aagataatta gtataagaac cttcaactgg
      241 attatttttt ctttcattta ttaaatcata taattctttc aatatatttg tttgtacttc
      301 aagctccatt tttgaactat catcaaacaa gtctctgtaa aaacaagaat aattgcctgt
      361 atgacaagct acaccagttt gttcaacaaa aagtagtata gtatctacat cacaatcata
      421 agacatctta actacttttt gagtattacc agatgtctca cctttaaccc aaagttcttg
      481 tctacttcta ctaaaatagc aagctacttt atcttttaag gttttcttta tagcatcttt
      541 attcatatat gctaacatca gtacatcttt actaactacc tcttgaacaa caacagggac
      601 taacccttta tcatcaaact ttatacttct tataaattcc tctgcttcat cactgtatac
      661 attgttacat ttattatcca tatttctaat ctccttttag tatttaactt ataatcttac
      721 ttctacaccc atgtctttta aatatttctt taaatcgttt atttttatct cgccaaaatg
      781 aaaaacagat gctgctaaaa ttccatctac attagactta ttaaccgcct cataaaaatc
      841 ttccattttt ccagcgcctc cagatgctat gacaggtaca tttactaaag aagttatctt
      901 tgataataat tctatatcat atccattttt cattccatct tcatccatac tatttacaac
      961 gatttctcct gctccaagtt ctacaccttt aacagcccat tcaattgcat ccagattagt
     1021 tttttctctt ccacctttta cgtatacgct ccatgaatct tcttcatttt tctttgcatc
     1081 tatagataaa acaacacatt gtgctccaaa ttttaatgaa gcttctctta ttaattcagg
     1141 atttttaact gctgatgaat ttatagaaac tttatcagca ccttttctta aaatatcagt
     1201 aaaatcttct aatttattta ctcctccacc aacacaaaat ggtatattta tattttctgc
     1261 aacttttgtc acaaattcta aggatgtctt tctatcttca tttgatgcag ttatatcata
     1321 gaatacaagt tcatctgcac cactatcact ataaaatttt ccaagtactt ctggactatc
     1381 aacatctact atatctttga atttttttcc ttttacaacc cttccatttc ttacatctaa
     1441 acaaggtatt attcttctag taagcataat tgttgtgcct ccttaaagtc tatcttttta
     1501 tcataaagtg ccttacctat tattgcacca tataagttca tagcttttag tctttttatg
     1561 tcttctatag aagttactcc tccagaagca ataacgttta atgacgtttc ttttgctatt
     1621 ttttcatata tctcaaaatt tggaccttgt aacatcccat cttttgagat atctgtataa
     1681 acaatagttt gtacacctat cttttcaagt tgtttacaca atgttaagga gtctacatta
     1741 cttacaactt cccaacctct aactgctacc ttcccatcct tagcatctat agaaactact
     1801 attttctctt tatattcatt gaccaattct tcaaccaaat ttaaattttc aactgctata
     1861 ctccctaata ttactctagt aacaccagca tttattaaac ttttgacttt ttccttatct
     1921 ctaactccac cgccaacttg aataggtata tctatattgt ttgctatgtc ctcaattatt
     1981 tttgtattta caccaaattc acttctagca ccatttaaat ctactatatg tatatattct
     2041 gctccttcat ttttccattt atatgctact tctaatggat tatcataata tacatttact
     2101 ttgtcaaatt ctccttgtgt gagtcttaca cacttattat cttttatatc tattgctgga
     2161 aatattatca ttttatcatc tccccatatg ctcttaatat atttaaccct acttctccac
     2221 ttttttcagg atgaaattgt ataccatata cattgccttt tctaacaata gcaggtattt
     2281 tcttttcata ttctgaaaat gcaactagtt cctcatttga tgaatttgca taatatgagt
     2341 gaacaaagta tacatagtca tcttcattta tgtatttaag tatatcatca tttgccttat
     2401 taaatttcag attattccat cccatatgtg gcacttttaa acttatatcc agtttatcaa
     2461 tacttccttt tataagccct aatccttcat attctccata ctcataactt ttttcataaa
     2521 gcaattgcat tcctagacat atacctatta tgaatttccc cttagaaaca tgttctttta
     2581 ttactggtat caaacctagt ttatctaaag cacttataga atctctaaaa gctccaacac
     2641 ctggaagtat aagtgaatct gcctgtttta tttcatttat atcactagaa attttagttt
     2701 ctattccagc ttttctaaaa cccctagaca cagaatctat atttccaaga ccatagtcta
     2761 ctattatatt catgctacaa aacccccttt gaagatgcta tctcattaga aactatttca
     2821 ctaccctctt ttaaagctct tgcaaatgct ttaaaaatac tttctatctt atgatggtca
     2881 ttttctccat ataagagatt gatatgtaat gttactctag attcatttac aaaagctcta
     2941 aaaaattctt taaagcattg agtactcatg ctaccaatca tttgggtatc aaatttagca
     3001 ttaaatacaa gataaggtct attgcttata tctattgcta ccattgatag agattcatcc
     3061 ataggtatat agatattaga atatcttctt actccttttt tgtcacctag agcattttta
     3121 aatgcttcac ctatagctat acctatatct tctacactat ggtggtcatc tacatatgta
     3181 tcacccttac aaatcacttt taaatcgaac ttgccgtgaa atgacattaa ggtaagcata
     3241 tggtctaaaa atcctattcc agtatctatt tcagctttac cagaaccatc aatatttaat
     3301 tctactaaaa tctgtgtttc aagagtattt ctttctacct tccaagttct catacctatt
     3361 tctccttttt caaaatatct cttatagctc caacaaacat agaattttct tctttatcac
     3421 caattgtaac tctgtaataa ttttctaact ttccaccaaa ttttcttatt aaaactcctc
     3481 tatcaataag tttttgtgat aaattttcga tttcagaata aaataatata aaatttgctt
     3541 gtgatttata tgctttaatt cccataccaa tcatttcttt atataaaact tctctttcct
     3601 cttttacttc ctttatataa gcctttacca catctttatt tctaagtgct cttgtagcaa
     3661 taaagtctga taatgaattt aaattatatg gaggtcttac tttctcaaca gaatttatta
     3721 aactactatt agataacata tatcctgttc ttattccagc caatccaaat gcctttgata
     3781 aagttctaag aactattaga ttatcatact taaaaacatc acttaacatg ctttcttctc
     3841 caaaatccat atatgcttca tctaaaacca ctagactatt agttgaatct aaaagcttta
     3901 ttatctcttc cctctttaat attgttccag ttggattatt aggattacat acaattacta
     3961 ttttaggatt attctccttt actttttcaa tcacactatc tatatttata actaaatttt
     4021 catcactttc aacacctata aatttagacc catttatctg actatatata gaatacatac
     4081 taaaacttgg agaaaaactc aatattactt catctttatc tacaaaagta tgtattatta
     4141 aatcaattat ttcacttgaa ccgtttccta ctagaagatt tttcttattt actcctgaaa
     4201 tatttatgta atctattata gattctttca aattactata agaatcttca ggatataaat
     4261 ttaaatctat atcactatct gaaatttctt taataagata tttaaacaat ctcttacttc
     4321 cttcatttgc atcaagtttt actttacagt ttacgtgatt tggttcatat ccccttaact
     4381 ctcttatgct ttctttttct ctcaacttaa atccctcctt ataaaactca attatatttt
     4441 aattatcaag ttcttcaaat aaacgaatta tattctgaat ttgttcatat ttaaatctat
     4501 agctagctct atttgatata attctagcac ttatattgca tatgtcttct aatatttcaa
     4561 gaccatttgc tttaagtgta ttgccagtct caactatatc tacaattaca tctgacaacc
     4621 ctactattgg agctagttct acagagccat ttaattttat tatttcaatt ttttgacctt
     4681 tctctttaaa atactttttt gctatattgg gatactttgt tgccactttt agatattcat
     4741 cttctctata tatgctatct ccctttaatc ctgctacagc aaatttacat tttccaaaac
     4801 ctaaatcata tatttcatat acatctgtct cattctctaa aatagtatct ttacctgcta
     4861 taccaaggtc agcgactcct ttttcaacat atgtaactac atctgatggc tttacatgta
     4921 tatatataat tttattttct tcatctttaa aaattagttt tctcgtatct gtgtctatac
     4981 tatctcctag acccattttt ttaaattttt taaatgattc cccttcaatt cgtccttttg
     5041 ctagtgctat agtcaaataa tccaaagcaa tccctccttc taaactttac ttatatacat
     5101 acttcattaa ttcatcaaca cttagtgtaa atccaatagc tggaatttct tttccatatt
     5161 ctttagttaa agaatcatat cttccaccac taagaatttc tttatatgag tttgggtagt
     5221 atcctctaaa cataattcct tcataatagt caagcatagt aatcattgat aaatcaaatc
     5281 tagccttatc taaaaagtta cattcctcaa ttaaattatt tacttgcttt aattcttcaa
     5341 gagcttgctt catcttatta ttacaataga acttgtttga tttttcaata acgttgtata
     5401 aattcccttg caagcttaaa atatttgata atagctcttt tacttcacgc ttaattttca
     5461 gttcttcaat atatactttt aattcatgag tgttcttcgt atatagtaaa tttttaattt
     5521 gattttcaca attcttattt aaatttaatt cttctaacaa accatgaata tacttgctac
     5581 tcccaacctc taaaataaaa ttattattgt atttttcaag tatcttaaga gctaatttca
     5641 ctacttctct atctgcttct acagaggctt ctcctagata ctcgcaacct atttgtctta
     5701 tctcttttat tccaactgta tttttatttt tatatattga tgaattataa aaaagcttaa
     5761 gctttaaacc atcttcccat cttgggacaa gactttttat tatacttgta gttatatcag
     5821 ctctaagcac cattactttt ccatttacaa cctttaccat agattcttct ggaattttat
     5881 tatttatagt tgtaaattca tcatattctt caaataattg tggttctatt tgaaaatatt
     5941 cttcatccaa aaaaagttta tctatttccc tttttaattt atatcttttt ttaaaataaa
     6001 taacctcatc ttcaatcctc aaaaatttca cattctcacc ctcttcatta aatttactct
     6061 tatatttgca aatttaaaac aataaaaaaa ccatataggc tttataccta tatggctata
     6121 attttctctt aaaattactt cccatcatag atacaagacc aattacacta aaaacagggc
     6181 ttgtgtctat gaattaattt catattacaa tccctttaat ctatgatgat ggtgatgatt
     6241 taattttgta tttaatttta ctatatttaa tctcaccatg ataatttcct c
//
LOCUS       DAEMAE010000206         6290 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEMAE010000206 DAEMAE010000000
VERSION     DAEMAE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.208"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            328..486
                     /locus_tag="KPH93_003103"
     CDS             328..486
                     /locus_tag="KPH93_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689877.1"
                     /translation="MKIFSFLLFVVGICFCIAGFIGHYPAFAGATLSFFATTMISVSQ
                     VKTKKKMK"
     gene            complement(773..1477)
                     /locus_tag="KPH93_003104"
     CDS             complement(773..1477)
                     /locus_tag="KPH93_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689878.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKTQQGNINNDVPKSFEVKNLKVDYENHIVSINDEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1468..3075)
                     /locus_tag="KPH93_003105"
     CDS             complement(1468..3075)
                     /locus_tag="KPH93_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG1689879.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYYEEGKDKWK"
     gene            complement(3329..4507)
                     /locus_tag="KPH93_003106"
     CDS             complement(3329..4507)
                     /locus_tag="KPH93_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689880.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4733..5227)
                     /locus_tag="KPH93_003107"
     CDS             complement(4733..5227)
                     /locus_tag="KPH93_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1689881.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5228..5989)
                     /locus_tag="KPH93_003108"
     CDS             complement(5228..5989)
                     /locus_tag="KPH93_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1689882.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFIGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2110 a         1171 c          683 g         2326 t
ORIGIN      
        1 cataaaaaga taaaaagggc aattttttta tatcaaaaaa aatattaaat tgatattatc
       61 catatataaa cttatattat ctgtataaat gttatattta acaattctac ttgcttccct
      121 tacacttatt agcatatagt tgtaagagct tgttacaaaa caacacttag cacactttaa
      181 aaagtgtggt ctgataagta gtcaacaaaa aagacataat aaagtataaa atatgatata
      241 cctttccatg tcaccaaaac tgatgatttt actattaata tatgctaaaa tatgcttaat
      301 aaattaaatt ttaatgggag aatagttatg aaaatattta gtttcttgtt gtttgtagta
      361 ggaatatgtt tttgtattgc tggatttata ggacattacc cagcatttgc tggtgcaaca
      421 ttgagttttt ttgcaactac aatgatatct gtaagccaag tcaaaactaa gaaaaaaatg
      481 aaataggcaa atcctaactt tacaagttaa tattacacaa gtaattagtc taaaattgga
      541 tactttttct attacagaaa tacatcatcc actttgtatt ctcttgaaaa aataaaatag
      601 ctatgtaaat ttaagattag atagactaaa tctctatcca tttcattgta aaatatacat
      661 agctatttgt atttgattta aaatacttag attttcttac ataacaaata atttctatct
      721 gattattact tatattcatt taacaaccac tcatcaacta ctcatttagt atctattcgt
      781 ccgccatacg gtaacctacc ccaacctcag tcaaaatata ttctggctga gcaggattct
      841 tttcaatttt ccttcttata cttgccataa aaactctaag cgactgattt tcactttcat
      901 agtaatttcc ccaaacttta tctattataa acttatgggt taaaactctt cctgaatact
      961 tagacatcaa ctcaataatc ttatattcta ttggagttaa atgtatttct tcatcattaa
     1021 ttgacacaat atgattttca taatctacct ttagattttt aacttcaaaa gactttggaa
     1081 catcattatt tatattaccc tgttgagttt tatttctaag agctacacgt actctagcca
     1141 aaagctctgt tacactaaaa ggtttagtta aataatcgtc tgctcctcca tctaaagctt
     1201 ccaccttatc cctctcatgc tcccttgctg aaacaacaat tattttatta gtctttgcac
     1261 atgctctaac tcgtcctatt acctcaatcc catcaatatc tggtagtcct aaatccaaaa
     1321 ttatcaaatc tgggtcaaga gaatgtataa gtgatatagc tgttcctcca ttatccgtct
     1381 ctacacacct ataattttga gtttcaagag atacttttat aaatttacaa attggcttat
     1441 cgtcttctac aattagaact aatggcttta tttccacttg tctttcccct cctcataata
     1501 cataggtatg ctaaatctaa atgtagctcc tccagaagga ttattaaagg cgacaatttc
     1561 tccaccatga gcttctacta tagatttaca tatctccaat ccaagaccaa tacctcttct
     1621 ttcaagattt ctagacttat tcttagtata aaacctatta aaaatattcc cgatatcttc
     1681 atttgggatt ccttttccat tatctgcaac ttcaaatagc acttgatttg actctttttt
     1741 tacagttagt tctatcttag aatttttagg tgtatgcctt atagcattat caattagatt
     1801 tactaaaact tgttctatta ataatacatc cacatgaacg attattatct catctggaat
     1861 atttgttttt atgtctctac tattggcaaa ctttttgact cttaatatag attcagctat
     1921 tatttcatca acaacctctg gtcgtttttc aatatccaat tttccttcat ctattctggt
     1981 catactaagt atattctcaa cagaatgagt tagccaactt gtatcttcat atatattttt
     2041 caataattct actctagttt ctttatctat cacatcatca ttttcaagta ttgttgaaga
     2101 tgaacccaat atacttgtaa gtggagttct taaatcatga gaaattgacc taagcaagtt
     2161 tcctcttaat ctctctctct ctgcctcaag atttgctttt ttatttttct caaataaatt
     2221 ttctctatca attgccaacg caacctgcgt tgaaactgct tctaatagta tcttttcatt
     2281 ttctgtaata atatctatct taaaacacgc aacacctata acaccaagaa catttgattc
     2341 ttgacctttt atagggaaat aatatgcact gctgttttta caatattttg tcccaattcc
     2401 aactgggtta cctgtcctaa aagcttcttt tatagcttcc ttttctaata ctgaatggaa
     2461 tatattctca cttccatcat tgttacaaat ataaatactt ggcttaccta gattattatt
     2521 ctcatctgca atagtcacta ttacagttct atttaatata tttactaaat tagacccaca
     2581 aaattcaata atttgatttt tattttttgc tttaagcaac gttttgttat tctcatagag
     2641 taatcttatc attttttctc tcttatgaga aagtctcact tctcttttta tcctagaagt
     2701 taaagtactt gttataattg ctgccatcag cataatacaa aaggtcatag gataatcttt
     2761 tctataagct aaaaaagtat ataatggatg cgtaaagaaa aaattaaatg atagcactcc
     2821 tattactgaa gaaagtattc caaaaaaata tccataggtt atacttgtag caaatagtac
     2881 acctaatatg tatactagta taatattaga ttcattaaaa cctacctttt caaaaactac
     2941 cgatattcca gtagcaattg tcataaccaa tatagttttt atgaagtctt taataattat
     3001 ctttttactg agatagccat cttcatcata atcatactct tttaagtctt catgttgtag
     3061 tcgttccttt ttcatttact acaattcctc cttattgtta ctgtttaaat tggctctatt
     3121 ttatataata caattttatc ataataaatt caatcaatac atttaattaa tgtacttaaa
     3181 ttactaaaag gtgctgtctt aaaatgaaaa taaatctcta tttcaaaaca gcacctatat
     3241 atttaaatta ctaatttagt ttttacatac ttaaatcaat agttttattc aatttcataa
     3301 attataatgc ttgaattatc gaacattctt atccaacaag cataaatata aagtgggcac
     3361 atatacctgc acacaagcca cctatggtat gcgaaagtat cgccttacca gccaatggtc
     3421 ttgcatctaa agcatccatc atacctatgt gtgtacttaa atatccactc cagcacatac
     3481 ccattgctgt gaatactgct atatcattag gagttatagc gcctgatttt ataaactctg
     3541 gtactaaaga tattgctgct ccaacagctc ctaatgcagt tacagggaat gctattgcct
     3601 ctggtgatgt gaatccaaat aatggttcta ttatgaagct aatcttatca ccaatagcag
     3661 gtaataattt tataccttca tatgcagctc ctgtgtaaac agcttgacct gtagcagggt
     3721 ctgtagatgg tccaaaagtt aataacataa ctaatgtaca aactactaaa actcctggta
     3781 ttatagccat acccatttca acacctaact taccaccttc aagtatagca tccaatgttc
     3841 tttggaatac attaccatct cttatttctc taaactcttc aagttttgca cctttatcat
     3901 ttatttgctt ttcagtatca tttttagggt cataattata atacttttta gtaaaagtca
     3961 acataattct aacactaata atactaccta ttattgcccc aacattacct attatagctg
     4021 gtagtacata ttctttacct tgagctatca taaaagttgt tacaataagt cccataccaa
     4081 aggctgttcc taaattacat aaagcaggta cttgatactt cttaaaatat tgtgtaaatg
     4141 ttttgtcttt agcaaatggt ataatagctg gattatcaga taaatatgtt gcaactactc
     4201 ctgctatact agcaccaggt aagccccata taggtctcat aagaccctta aatatcttat
     4261 taactaaaga tattactcca aactctgaaa gcaacgcact aagtgcacct gcaagaactg
     4321 ccatagccat tattaaaaat actgtgtcta ataaaagcgc atgtgctgta ctcattatta
     4381 ctttaaacat cattcctgca cccatgatgc ttcctacata tccaaatcct accgccaata
     4441 gtacaataaa tacgaaggtt tccatactta ctgccttgac tcttctctta tccgacagct
     4501 tttccatctt gaaatcttcc cccttttaat tttaaattaa ggttcctaac attggtacaa
     4561 attttttggt acaaactgag ttctaaaact tacactcgta atgcaagttg tcagtttttt
     4621 gaaataaaaa aaaattacgt ttatagaata agaatatcct ctttaatttg gatactctta
     4681 ttctatttta tgttattata ttatcatcct tttgtttaaa agtcaatttt ttctattttt
     4741 gttgtttaat tttatttata ctctttttat atttttcatt ttcttttatc tttgtaagca
     4801 ctccatataa gttgactttt ctaaatacag tagttataaa taccaatcct aatgcaattg
     4861 aacttgcaca tgataatgta ctcacaccat attctacagc ttttatataa ttattggtca
     4921 aaacagaata catagtatta tatattccat aacctggtac taatattatt aaacaacaaa
     4981 cacttaatgt agttgctgga gtcatataaa ttcttgcaca taactcacaa tatatcgcaa
     5041 aacaaactgt agctataaaa aaacataagt tttctgttac tcccatattt aaaagaaatt
     5101 tatatgaaaa ccaagctata cttccaccta ttcctgctag tattaagttt ttccctttca
     5161 tattaaatag cactccaaag cttaaggcag taaaaaaagc tgctattact tctataaaca
     5221 aatgcactta tatcccccct aacttgaagt acaatccaag taccaagaac attcctactg
     5281 ctatagatac acctataaaa aatacttctg ctaattttgc aagaccagaa atcaactgtc
     5341 catctaacaa atctcttagt gcattagtca aagcaagccc gggaactaag agcattattg
     5401 ctcctgctat taaatggtct aatgtcttta taaatccgag ctttaaaatt aaaattgaac
     5461 aagaagctat aataactgct ccaattgcat ttacaaaaaa atcattaagg gaagatttac
     5521 ttaaatacat aataacaaac ttagtcagaa tcccaacagt aaatgcagct ataaattctt
     5581 ccacatttcc tccaaataat atagaaaaag ttgctgctgc aatgcctgca aaaaatattg
     5641 ttattaaatc cgagtaatag ttatcttcac ttatttcatc taaaattttc tcacataatt
     5701 caatatctaa attatttata cttattgctc ttgataatga gttaacttca tgaaccaagt
     5761 ttaggtctac tcttcttgag cttattcttt taattgctga atgtaatttt ccatctacaa
     5821 atacagatgc cattacagct gttggagttg caaatacatc tacatggtct attccaaaag
     5881 attgacatat tctagctatc gtttcttcaa ctctataggt ttctcctcca ctttgcaaca
     5941 tagctttacc tgcatttgag gaaaagttta aaattctatc tatatccaca aaacatatct
     6001 cccattcttt tatagtatta ttcatatttt aatacaagat attatttatt taaactattt
     6061 taaataattc atatccaata tttacatttt atgaaaaaac ttgtttttta tgcctgtcaa
     6121 aaccaaattt taaggttgtt tacaccataa aatggctgaa ttaatatttg acaaacactt
     6181 tcaaccattt atataacctt ctaaattatg tgatttttta ctacaaataa aattatatct
     6241 cttttaattc tttctcttga gtatttaagt ttacaacaac ttcatattta
//
LOCUS       DAEMAE010000207         6178 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEMAE010000207 DAEMAE010000000
VERSION     DAEMAE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.209"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(217..738)
                     /locus_tag="KPH93_003109"
     CDS             complement(217..738)
                     /locus_tag="KPH93_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689883.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENEKTNSYYFNRMLENSRSHHLVN
                     IEWFEKLLNDIKD"
     gene            complement(790..1638)
                     /locus_tag="KPH93_003110"
     CDS             complement(790..1638)
                     /locus_tag="KPH93_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBG1689884.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNSNYKFYYKKLLSIIEALRKELNVPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDFALGKISYDEFESKL
                     IKLNDN"
     gene            complement(1785..2066)
                     /locus_tag="KPH93_003111"
     CDS             complement(1785..2066)
                     /locus_tag="KPH93_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689885.1"
                     /translation="MPKIEKDLDGTTTITYLNDDVDVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            2460..3314
                     /locus_tag="KPH93_003112"
     CDS             2460..3314
                     /locus_tag="KPH93_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1689886.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSF
                     IKKGEITTLKAFPPISFKINIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQPQGVLGISICNNSEKWKYFISVSSTKSIDDTLEEYT
                     VPAFTWAIFSGEGQCPQAMQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            3514..5019
                     /locus_tag="KPH93_003113"
     CDS             3514..5019
                     /locus_tag="KPH93_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1689887.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMIELLDEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYNLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLNGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSSGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNVLLKSVINYAVYKKEPNQKSGDFSLVLYPNIPRN"
     gene            5046..5546
                     /locus_tag="KPH93_003114"
                     /pseudo
     CDS             5046..5546
                     /locus_tag="KPH93_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5706..6053)
                     /locus_tag="KPH93_003115"
     CDS             complement(5706..6053)
                     /locus_tag="KPH93_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1689888.1"
                     /translation="MHENKKNLLDGSLAQYNDAAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGENECDVKNGDTVFVPTGEWHEITNGSNEEMIAV
                     QITKINAGAEYRG"
BASE COUNT         2312 a          839 c          850 g         2177 t
ORIGIN      
        1 tttattatat caaatgtcat acttttcatt ttaattgtct gtaattcaac aatgaacaac
       61 tttatattta taaattttac ttacatagta taagtgtctt ttaacacctt atataacaaa
      121 aagacagata ttcaattgaa aatcatctgc cttattttta atctatatat catacattta
      181 tataaagtat atatgtatat ttttttatta ttaaaatcaa tctttaatat catttagcaa
      241 cttctcaaac cattcaatat taactaaatg gtgagaacga gaattttcta gcatcctatt
      301 aaaataatat gaatttgttt tttcattttc tatggattca tttaatagag aaattcttac
      361 ttgagtttgc ttaatttgcg tttccaataa ttctattaac ttttcatttt tataatcatt
      421 ggcaaataat agtgttgcgt aaaatgatag atttatatac tctgttttag aaccatactt
      481 atacattagt ttttcaaatt cctgacatcc aagttcagta attatgtagt tatgagtttc
      541 tctattttta ttagattgtt tattttcaac tttttcaata aacttttttt cttcaagttg
      601 ctgtaaatta taataaaaag aacctttagt gaaatttact atgtacttgt aatgcttctt
      661 ttccattaat gctaataact catatccata agaatttgga ttttgtttta ataatcctaa
      721 aattaataag ggtatcaaaa aatcacctct ttcaatctat atttactagt atattaatga
      781 atattttatc taattatcat taagtttaat aagtttagat tcaaactcat catatgatat
      841 ctttcctagt gcaaagtcca tactatttat atatattttt tctgtttttg tatatgtctt
      901 ggaattactc ggtattatca actcactttc attagctaat gcttccataa tatgcttttt
      961 cttatgaaaa gcttcaaaat aacgtaatcc aaattttctt tgagatattg catcaatatg
     1021 aataccatca ggattagatg taagacctgt tgctgtaaca aagtaacaat tatcttgttc
     1081 aaaagcaaat ttctgtaact cttgattgat aaatacgtac tcagtacaac tttttccaaa
     1141 acctactttt cctaagaagt ctcccaatcc accaataata attggtatat ctggaacatt
     1201 taattctttt ctaagagctt caataataga aagtaatttt ttataataaa atttatagtt
     1261 gctattatta ctgtcatttt ctccctgatg ccataaaatt cctgtcaatt cactgctttg
     1321 tatggcaaat ttagcttcac ttatagcatg tttaaaaagt ataccatcta tattccattc
     1381 atctaaggaa ctacctccct cagcacaagg aattagccca attctatcct cttgattttc
     1441 acaacaccat gcatctgcaa atgatgcagc aagacttacc ccagaaacgg gtctatcata
     1501 attaataggt tctgtcatca tttgccatct tccattacgt aacatctgta ttctttcatt
     1561 ataaattgga gtcacttcac ttatgaatcc tcttccagcc atattggact gccctaacat
     1621 taaaaatgat ttaatcattg ttattatcct ttcttaaatt cttattgtat ttatttttaa
     1681 tagtagtcta attagactat ctatgtttat attatagtct aattagacta taagtcaata
     1741 atattataaa aaatacttgt cctagttcac ttattaaaga agtgctaatg actagcaact
     1801 tctttgtttg aattttttat ttgtaattgt ctgtctacca ttattttctc tattgctcca
     1861 tatattattg tatctttttc ttgtttttct tctagtgtga actttcttcc taagattgtt
     1921 tcaggagata ctacatggca gattgtcttt ccatgtagta ttgtcataga tattttataa
     1981 ccattctcat ctgttggaac atctacatca tcgttgaggt atgttatagt agttgtacca
     2041 tccaagtctt tttctatctt tggcatttaa atcccctcct ctttatattt tatgtatctt
     2101 aaaaattgtc ctattctcaa tcattattta taattgagta actaagtgtt gcttgattaa
     2161 aggtaaactt caaaaatagt taactatgaa taaaatgtta tatataatta aatatctaag
     2221 ctaaaatatt ataaaaattt cataataaat taaaactgtt atcttatata tcttatataa
     2281 aaattagata ctaatttgtg atatacttta atgtgaaaaa caatactatt tgttcatttt
     2341 tgtagttaat tacagacttt ctcatgttca ttctttatta agctagttaa ttagtgcata
     2401 gaaagagagg attaactatt tcaaaataga tattatataa attacaagga gctgatttta
     2461 tggaatggat tgaaagatta aacaaagcaa ttaactatat tgaagaaaac ataacaaaag
     2521 aaattgaata tgaacaagtg gcaaaaatag cttgttgttc tacatatcat tttcaaagaa
     2581 tgtttgctta tatggcagat gtaccacttt ctgaatatat tcgtcgtaga cggatgtctt
     2641 tagcagctgt agaattacaa aatgatgata aaaagataat tgatgtggct ttaaaatatg
     2701 gatactcttc tccaacagca ttcaaccgtg catttaaaag tattcatggt gttgccccat
     2761 catttataaa aaaaggagaa ataacaactt taaaagcctt tcctccaatc agctttaaaa
     2821 ttaatattaa aggagccgaa gaaatgaatt atagaattga aaagaaagaa gcatttcgta
     2881 tagttggtgt atctcagcca cttcacaatg aactagaaaa gaattttgaa attgttcccc
     2941 aaatgtggca aaaagttgct ttagatggaa cacttcaaaa attaatccct atgatggata
     3001 gtcaacctca aggtgtatta ggtataagta tttgtaataa ttcagaaaaa tggaagtatt
     3061 tcatctctgt ttcaagcaca aaatctatag atgatacatt agaagaatat actgttccag
     3121 cttttacttg ggcaatattt tctggagaag gacaatgtcc acaagcaatg caagaactag
     3181 aaaagcgtat tgtaacagaa tggttaccta cttctggtta tgaatatgat aatggtcctg
     3241 atattgaact gtacttaaat ccagaccctc aaaatgctaa atttgaagta tggatacctg
     3301 tagtaaaaaa atagtttaaa attaaaaaat gaagaaagca taagattaat agtataaata
     3361 tttacctaga ttacaagctt ttaaatatac tataaaattt actgtataaa aataaaccta
     3421 aaaaatatat cttgtatttt gaaggtttat ttttgtgttt taaatataat aaatctgtta
     3481 ctgaatataa ttatattcaa ggggtgtgat ttaatgaatg tggcaattta tttacgcaaa
     3541 agtagagctg atgaagaagc agaacataga ggtgaatttg aaactctaag tagacataag
     3601 acaactcttt taaaactagc aagagaacaa aatctaaatg tcattgaaat taaagaagaa
     3661 cttgtttctg gggaaagtat agaatataga cctaagatga tagagctact agatgagatt
     3721 aaaaataata actatgatgc tgttttagta atggatattg accgtttggg tcgtggaaat
     3781 atgcaagacc aagggctaat actagataca tttaaaaaat ctaagacaaa aattattact
     3841 cctagaaaaa cgtataactt aaatgatgag tttgatgagg agtattctga atttgaagct
     3901 tttatggctc gtaaggaatt aaaattaata tctagacgta tgcaaagagg aaaaatcaaa
     3961 agtattgaag aaggtaaatt tatagctcaa gctcctcctt atggctatga tattaaatat
     4021 ttaaataata aagaaagagt acttgtaata aatgaagata aagcaactgt tgttaaaata
     4081 atctttgata tgtatttaaa tggagatggt gcatgcaaaa tatccaatta tttaaatgaa
     4141 cttggctata agaccacaac aggtagaact tggtatgaaa agggtataag agatattata
     4201 aaaaacaaag tgtattgtgg atatatagta tggaataaag tcgagaggaa aagaaattct
     4261 agtagaacaa gaggaattga ggaacaaatt gaggtaaaag gaaagcatga accaataata
     4321 agtgaagaca tatggaataa ggcacaaaat ataagaaaaa aacgtattaa ttcccctact
     4381 ccaaacaata aaataacaaa tccattggct ggaattgtaa aatgtggata ctgtaatcat
     4441 actttaatag ccaaatctaa tactacatca agtggatatg agaaatatat ggtttgtact
     4501 aattgtaaaa ataaagctac taaactatac atattagaaa ataaaatatt agaggtctta
     4561 aataaatggt tagtaaatta tagactatct ttaaaagatg aaaaagacaa ctctaaacct
     4621 gaaaatactt tagaacatac cttgataaat ttaaataaag aattggatac tttagaaaaa
     4681 caaaaagaac gactacatga ttttttagaa caaggaattt atgacagtaa tacatttggg
     4741 gaacgtttaa aaatactctt gtctcgtata gaaaatgtaa gagaagctat taataaaaca
     4801 aatatagata ttgagatgca aaaggaaaga aatacagtta aaacacaaat tgttcctcaa
     4861 cttgaaaaaa ttttaagtga atatacagag gagaaagaac cttatctaaa aaacgtattg
     4921 cttaaaagtg taataaatta tgcagtctat aaaaaagaac ctaatcaaaa atctggtgat
     4981 ttttctttag tactttaccc taatatccca agaaattaat tatatatttt aaatgttata
     5041 aatagactga ttaccaattt ggtgtaatgg ctggagcttt tgcaggtgga gagggtgact
     5101 ttacaacagc atttgaacct acagcaacag caatggaaaa agaaggtagt ggatatatag
     5161 tcacatctat aggaaaagat tctggagaaa taccttttac agcatactct actacgagat
     5221 catacatgaa aaacaataaa gatttaattc aaagatttac aaatgctgtt tataaaggtc
     5281 aattatgggt tcaacaagca tctagcaaag aagtagctaa ggctatacaa cctttctttg
     5341 aagatatatc tttagaagat ttaacaacag ttgtagatag atataaatca atagatgcgt
     5401 ggtctcaaaa ccctgtatta aaagaagaag gtttgaagaa attgatggct gttatgaaag
     5461 aagctaaaga actagataag gaagcacctt atgaagatat tgtaaatact gagtttgcaa
     5521 acaatgctat aaaaaatata aaataaaaat gtgcttaatt ttaaccaaat atttttctgc
     5581 ttttatgata aaaattaata aatttataaa aatataaaaa gagtctttta aaagactctt
     5641 tttatatttg agattaaagt ttatataata aaatagaaat caaagtacta gttgtaccta
     5701 tatatctatc ctctgtattc tgctccagca ttaatttttg taatttgaac agctatcatt
     5761 tcttcattac ttccattagt aatttcatgc cactctcctg ttggcacaaa tacagtatcc
     5821 ccgtttttta catcacattc attctcacca agtttcattt taccttctcc ttttaggaac
     5881 acaaatattt gttcaccatt agggtgtcta tgagcattaa gaacttgatt aggtttaaaa
     5941 taataaactt ctgaagttat atcgttacca gcaaatactg gtatttttgg aactgctgca
     6001 tcattatatt gagctaagct tccgtctaat agattctttt tattttcatg catactagaa
     6061 atacctcttt tctatattat agatttacta ttattatacc aatataagaa aatagtattc
     6121 tcactttaca caataggaat aaaattcttt tatttttaaa tacaagtata gtactatt
//
LOCUS       DAEMAE010000208         6172 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEMAE010000208 DAEMAE010000000
VERSION     DAEMAE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.210"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..764
                     /locus_tag="KPH93_003116"
     CDS             114..764
                     /locus_tag="KPH93_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG1689889.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKGIVKKMGNTYEILNYDELHRLAFEVKEFLEDE"
     gene            complement(904..2280)
                     /locus_tag="KPH93_003117"
     CDS             complement(904..2280)
                     /locus_tag="KPH93_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1689890.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2296..4209)
                     /locus_tag="KPH93_003118"
     CDS             complement(2296..4209)
                     /locus_tag="KPH93_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1689891.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLSSLYDEWEEFM"
     gene            4345..4977
                     /locus_tag="KPH93_003119"
     CDS             4345..4977
                     /locus_tag="KPH93_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG1689892.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5116..5655
                     /locus_tag="KPH93_003120"
     CDS             5116..5655
                     /locus_tag="KPH93_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG1689893.1"
                     /translation="MIFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5832..6002)
                     /locus_tag="KPH93_003121"
     CDS             complement(5832..6002)
                     /locus_tag="KPH93_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1689894.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2272 a          853 c          872 g         2175 t
ORIGIN      
        1 atggatttgt caagtttaat ttagttgtaa ttctatcttt tattaaaaat aacataaatt
       61 aaaacatata aataaaatat tttatacaat atattgtaaa ggaggtaact ttgatgaaag
      121 cagaaaaaac aagtatatat ccaattatta aaaataattt aaacaaaata tcaattgttg
      181 aatataaaaa gggtcaaaga tttgtagcat cagataaaga cttacaagaa gtgttcttta
      241 taatagaggg agttgcatta gtggagtgta ctactagagg aggaaataaa tttcttgttg
      301 acattgtacc tgaaaatgaa tttataggca aaatcagtta tatatatgaa cataatttga
      361 aatgtgatat atttgcaaag accaatataa aattatttcg atttgaaaaa aatgtatttg
      421 aagagtttta tctcaaacca gattttattg cactatttca cagaaaatgt acaagaagaa
      481 tatatgaatt atataaaact agaatggtaa gagaactatt ttcttgcaca gaagttattg
      541 cctattgtat atcaaataaa caagaaaata atgtttgcaa tataaaagct attcgtaatt
      601 tcagtgaaat ctattcaatt agtagaaaaa gtagatacca ttctctgtta aagttaactg
      661 aaaaagggat agtaaaaaaa atgggtaata catatgaaat tttaaactat gatgaattac
      721 atagattggc atttgaagta aaagagtttc tggaagatga gtaaagattg ttacttaatc
      781 aaaataaggt ataataattt ctgtaaaaaa tagaaatagt ctcactgaaa atcaaaaata
      841 ctttagttat tttcaatgag actgtttaga aatattgtat aattacaatg tatagtataa
      901 ttattagtca atttccttta ctatattaat agcccatttt ttagataatg ctctaggaac
      961 gccaaatata aacttaacca cttcttcact ataagtgagt aaaacgagta caaatatagg
     1021 tgcaccttta aatgcagcta taaaagttag aggaatagaa gctaaccaca tacatcctaa
     1081 ttctaaaaat agagcatatt tagtatctcc tccacttctt aaaataccaa ttataaccaa
     1141 tgtattaaaa gatttaagag ccattagtat tcccattata aagaatatct tagttatatc
     1201 agaagctaag ctatcagaaa cactaaacat ttttaataat aatggtatat ttaaaattaa
     1261 aataattcca agtattaaac cagctgaaaa tacaagtata gaaaactttt tagcatattc
     1321 aatggctctt ttaatatggt cagctccaag ttcatttcca agcataatag atgcaccaga
     1381 tgcaatacat acagctgtca ttatgaagaa attacctgta ctggttgcta tctggctagc
     1441 agctatagca gaagtaccag ctcttgcata tgcaacagaa tacaggacag ttccaattgc
     1501 ccatacagag tcatttacaa atataggaaa gctttttgat gaaaaagatt ttataaaaat
     1561 tttatcaata gcttttagat tttttagacc aaactttaat atatagtctt ttttataaag
     1621 gtaaacataa gtaatcatta aaattaattc acatattcta gcaataacag tagctagagc
     1681 agcacctttt acacccaatg caggaagacc gaagttacca aatataaatc cataatttaa
     1741 aataacattt gttacaagtg caaatgcact acaaatcata cctaacttag gatttctaac
     1801 acctcttgaa ccagtactaa atacagtact tacagcaatt aatggataac taaatactgc
     1861 gatactaaag taatctatac aaagttttac tacctcagaa tcatacgaaa atatgtgtat
     1921 aattggcttt ggtgttagta aagcaggaat aacaaataca aaacttaaaa caattgccaa
     1981 tacacaagta agacctgtga cttttcttat attactcaca tccttctttc cataaaactg
     2041 tgaaataaaa actccagcac ctcctgtgat accagataag gacatattaa aaagaaagaa
     2101 aaattggttt gcaactccaa ctgaagctac agatgtttcc cccaaactac ttatcataat
     2161 tgtatctatt acatttacag aagtagaaat taagttctgt atgataatag gtatacataa
     2221 agatagaagt attttataaa atcttttatt ttcgtctttt aaagaaaata gttcttgcaa
     2281 ataatcctct cctttttaca taaattcttc ccactcatca tatagagagg aaaggttatt
     2341 ttctaaatta attttttctt ggctaacttc tttagcttta tctggattag agtatacttc
     2401 ttcttgacat aaaagtatat ctaatttttc aatttcaatc tctaactttt caatttcttt
     2461 ttctatattt tgtcttttta ttctattttt cttttctatt tctctttgtt ctctctcttt
     2521 tcttttttca tcttttattt gagttctagt tttttcttct ttttcttcta taatactcat
     2581 ctcttggatt tgcctttttt tatctatata ataatcataa tttcccagat attcagtaat
     2641 tccattttca tctaaaacta aaattttatc aacaacagta tttagaaagt atctatcatg
     2701 tgatattgta aatatagtcc catcataatt agttaatgcc tcttcaagga cttccttaga
     2761 atcaatatct aggtgattag taggctcatc taataacaat aagtttgcat ttgatagtat
     2821 aagtttaagt atggcaactc tagctctttc tccaccactc aatgtagaaa tcttcttaaa
     2881 aacttcttca tctacaaata aaaatgcccc taacatattt ctcaaagtag tttgagtaag
     2941 atgagtatta ttttcccaaa tctcatcaat tattgtgtta tctaaattaa gagttttttg
     3001 ttcctgatgg aagtaggaaa catgtacatt agttccaaat ttaatattgc cactaagagg
     3061 ttgtagttca ttagttatta ttttaaataa tgtagattta ccaacaccat ttgcacctat
     3121 aagtgcaact ttttcccctc tatatatatc taaatccaag tctttaaaaa gtattctttc
     3181 tccataaccc atagatacgt ctctaatttg aagaacatca ttaccactct ggacagaagg
     3241 cgtaaattgt atacgagctt ttttcctata agcttctggt ttatctaaaa cgtctacctt
     3301 gtctaatgct ttttctttac ttcttgcacg ctttaggtgt ttttctctac cataagcttt
     3361 tagtctttca attgattctt cttgcttttt tatttctttt tgttggtctt caaatttctt
     3421 aagctctagt tctttttcta ttttagataa ctcaacaaat ttagagtagt taccattata
     3481 tactttaagt tttttgttgt gaacttcaaa aattctattt acggattggt ctaagaaata
     3541 tctatcatga gatattaaca taacagtccc tttatattgt tttaaaaata cttctaacca
     3601 ttctattgct tcagagtcta agtggttagt aggttcatca agtaacaata gagttggttt
     3661 cttaagaagt aattttccta aaagaactct agttttttca ccaccagaaa gtatgcttat
     3721 tggcttatcc atatcattgt cactaaatcc taatcctttt agaactcctt tggcttcaga
     3781 tttatatcca tatccattta aatctgaaaa taattctagt ttatgtgaat attcatccat
     3841 gattttttct ataattggag agtttgtttt tgtactttct tcagatattt taacctccaa
     3901 ttctcttaaa taggattcca tttctatcaa atttttaaaa acttctagta cttcttccaa
     3961 aatagtatta tccgaataaa aattggtatt ttgttctaaa tatccgattt cacaatcttt
     4021 agaagtgtaa atatctccac tatcatatcc ataaattcct gaaattattt taaataaagt
     4081 agtttttcca gtaccattaa cgccgattat accaatttta tcgccctcat ttactgtaaa
     4141 actaatattt tctaatatag aatctatacc aaaactttta tttaagttat tacatgacaa
     4201 aacaatcata aatttctacc tccttgaact atctaaaatt ataatgtttt gatataatat
     4261 aatcaaggtt atatgtatta gttttgaaaa agaaatattt tattgtgaat caaacaaaaa
     4321 agaggacact tttaggaggt gaagatgttg ggaaataaaa atatatcaat ggcagttata
     4381 agaaggctcc caaaatatca tagatatctt ggagacttat tagataggga tatacaaaga
     4441 atatcttcta aagaattgag tgatataata gggtttaccg cttctcaaat aagacaagat
     4501 ttaaacaact ttggtggatt tggacaacaa ggatatggtt ataatgtaga agctcttcat
     4561 actgagatag gtaaaattct tgggttggat cgaccataca acgcagttct tgtaggagca
     4621 ggtaacttag gacaagctat agccaattat gcaggattta gaaaagctgg attcgagata
     4681 aaagctttat ttgatgcaaa tcctagaatg ataggtttaa agataagaga gtttgaagta
     4741 ttagattcag atactttaga agactttata aaaaacaata atatagatat tgctgtatta
     4801 tgtataccta aaaatggagc acaagaagtt attaatagag ttgtaaaagc tggaattaaa
     4861 ggtgtatgga attttgcacc tttagattta gaagttccga aaggtgttat agttgaaaat
     4921 gtaaacttaa cagaaagttt atttacttta tcgtatttaa tgaaagaagg aaagtagaca
     4981 acttaaacat attaataatg agtagttgtg aagagtattg agaaagtcag aagatttaat
     5041 attaaatcct ctggcttttt gaaatattag gataaaaatt taaataatgt atggaaataa
     5101 acaatattaa ggagtgtgat ttttgtggag gaattatgga aaaaggcaat tgaatttcat
     5161 ggtcatgaat gtccaggctt agctatagga tttaaagcgg cacttgcagc aagaaaatat
     5221 ttaggatgta agcagtcagg agatgaggaa attgtatgta tttctgaaaa tgatgcttgt
     5281 ggaatagatg gtatacaagt agtgctaagt tgcacaatgg gtaagggaaa tttactactt
     5341 aggatgactg ggaaatcagt atatcatttc tttaatagag aaaatggaaa atctataaga
     5401 atatatgtaa agtcaaagcc atttgagatg gatagagaag gatacaaaag atacttatta
     5461 gaaagtgatt ttgaagagat atttgagatt acagatacta agttaagact tcctgaaaaa
     5521 gcaagaatat ttaaatcact taaatgtgaa gtgtgtggtg aatttgcatc tgagcaatat
     5581 ataaggattg aagaaggaaa gaaagtatgt cttgattgct ttgataaata tgaaagattt
     5641 tatgaggagt attaaaatta taaattagat taaagttaaa agtattagat atgtgctaac
     5701 agtattttaa gatgaattag gtagcaacta taataaaata tataaataat attacttgaa
     5761 tcatacataa ttaaaaagag gctgattagt atttagaaaa taaaatcagt ctctttatga
     5821 ataacgaatt attattctgg ttgaggtgca tctacaggac aagctcctgc acaagaacca
     5881 cagtctatac aagaaccagc atctataaca tatgcgtcgt ctccagctga tatacagcta
     5941 acagggcatt cagcctcaca agcaccacag cttatacaag cgtcagtaat tttatatgcc
     6001 attttgaaat cctccctttt atatgtattg taattcaata acattataac aaatattatt
     6061 gaatataaca attaaattaa ttctacaaca aaaactttat tatgatattc ttgttttaaa
     6121 gttttacaat aaagtttaat ctgcttatat ttttatttat tttaaagaag tt
//
LOCUS       DAEMAE010000209         6102 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEMAE010000209 DAEMAE010000000
VERSION     DAEMAE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.211"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            199..897
                     /locus_tag="KPH93_003122"
     CDS             199..897
                     /locus_tag="KPH93_003122"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG1689895.1"
                     /translation="MNVIGIIGAMDEEVNILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            920..1921
                     /gene="trpS"
                     /locus_tag="KPH93_003123"
     CDS             920..1921
                     /gene="trpS"
                     /locus_tag="KPH93_003123"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG1689896.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEDIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            2508..3710
                     /locus_tag="KPH93_003124"
     CDS             2508..3710
                     /locus_tag="KPH93_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1689897.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFNPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDTGT
                     LIVFYVKE"
     gene            3715..4485
                     /locus_tag="KPH93_003125"
     CDS             3715..4485
                     /locus_tag="KPH93_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689898.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNGNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKDFRLLMHI"
     gene            4631..6052
                     /locus_tag="KPH93_003126"
     CDS             4631..6052
                     /locus_tag="KPH93_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG1689899.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIIVAI
                     ILGILFFKKAKHIKEQNNHKLAS"
BASE COUNT         2345 a          642 c         1118 g         1997 t
ORIGIN      
        1 tgaatgggtc tttgaggagt aagatgaaat gtaatttaag attacagagt atttgatatc
       61 gtgtatgcac gttatagcat atattaagat atatatattc gattttgagt gtatataaat
      121 ttaggtggta ccacggaaaa tatatccgtc ctattgattt aggacggatt ttttaatata
      181 gaaaatttag gaggaaaaat gaacgtaata ggaattatag gtgcaatgga tgaagaagtt
      241 aatattctag ttgatttaat ggatattaga gagactataa aaaaagctag cttagaattt
      301 tataagggaa ttttggaagg taagaatgta gtattagtaa agtgtggcat aggtaaggtt
      361 aactctgctc tttgtgccca gatactaata agcgaattta aggttgatgc tattgttaat
      421 acaggtgttg caggagcttt aaacgaaaaa cttgatgtta atgatatagt aatatcaact
      481 gatgctatac agtatgatgt agatactaca gcatttggag accctaaggg agttatacca
      541 agaatgaaga cttctgtatt taaagcggat gaaagactta tagatgctgc ttacaaatcc
      601 tctgtggaag aagtaaagac tcataaagta ttaaaaggaa gagtagttac aggagataaa
      661 tttataaatt ctaaagaatt aaaagaagaa ttagtaaatg attttggtgg atattgtgga
      721 gaaatggaag gtggagctat agctcatgta tgctatttaa ataatactcc gttcgtaata
      781 ataagagcaa tgtctgataa ggcagatgga agcgctgatg ttacttacga tgtatttgta
      841 catgatgctg ccaataactc taaagatata gttttaaata tgttaaaatc aatataaata
      901 tataaggagg tacaaaaaaa tgagtgaaaa aaaagttata tttagtggag cacagccatc
      961 aggcaaaatg actttaggaa attatttagg agctataaaa aactggactg aacttcaaga
     1021 taattatgat tgctattata gtatagttga tttacatgca ataacagtac ctcaggaccc
     1081 taaagtttta agagcgaatg ctatagaatt acttgctcaa tatatagctt gtggattaga
     1141 cccagaaaaa aatactatat ttatacaatc acatgtcaaa gagcatgttg aacttatgtg
     1201 ggtattaaat actatgacct atatgggtga attaagtaga atgactcaat tcaaagataa
     1261 atcacaaaag agtgaagcaa atctaaatgc aggtttattc acatatccag ttcttatggc
     1321 tgctgacata ttgttgtatc aaactgacct agttccagtt ggagatgacc aaaaacaaca
     1381 tttagaatta gctcgtgatt tagcaaatag gtttaataat agattttcac ctacttttgt
     1441 agttccagaa ggatactatc caaaaggtgg agcaagagtg atgagtttgc aagagccaac
     1501 taaaaaaatg agtaaatccg attcaaatga aaatgcattt atactattag cagatgattc
     1561 agattctata aagagaaaga taaaaagaag tgttactgat tctttaggtg ttgtaaaata
     1621 caatgatgaa caaccaggta ttaaaaatct aattgatata tattctaatt taagtaaaaa
     1681 gagtgtagaa gatattgtaa acatgtatga aggaaagggt tatggcatat ttaaagaaga
     1741 tgtggctgaa gtagtatcag aagctttaag acctataaga gaaaaatatg ttgaccttct
     1801 taataataaa gattatttag aaaaaatata tagtatggga gcagaaaaag ccgaaaaaca
     1861 agctagaaaa actcttagaa aagtttataa aaaagttgga tttatagaga gaagatacta
     1921 attattagct atctttactt gataaataaa ttttaaagtt attaaaaaag tatatgatat
     1981 gaaagagaga gtctcaaagg aaacttttgt tcatgaggca ctcttttttg tatgaaataa
     2041 aatatatttt aatcatttca acaatgaaaa agaggactat ctctattttg agacagccct
     2101 tttgataata aaaaaagatg agcttttgct catctcaaag gggttagatt attcttactt
     2161 tggggaaagt agaataaagg taaaagttaa ctgatgtcaa ctttgtcttt tttgcatatc
     2221 actactgagc ataaaaaaat cagaaaacca tagccttctg aaacaacttc taacatacat
     2281 tgtattttat acccaatatg taatttatga gttaatttta actcttataa tgaaaattgt
     2341 caataatata tcaaaaaaat attttttaaa tttatataat taaatgaatt tatgtttata
     2401 tataggtgat atgatacaat aattatggaa tttattttta tgtatctaac ttattacaag
     2461 aataataaat ttaaattaag taatgtttta ttttttaaag ggggattatg aaaacatcaa
     2521 ttttaatcgc attaatagaa aagacaagcc tcattgtagt actgtttctt ctaataacaa
     2581 agttaaaaat atttaagcag atatttcaaa aggaagaata tagttttaat gacttggtct
     2641 gtatagcatt agtttttact tttttagcca tatttggaac ttatagtggt ataaattata
     2701 tggggtcaat agtaaacact agaataatat ctatagtatc aggtggaatt ttatttggtc
     2761 caatggtagg tattacagca ggagtttttt cagggattca tagatatttt atggatattg
     2821 gggggataac atctgttcca tgtcttttat caagtatact agcaggtgta ttatcaggat
     2881 ttctatataa gaggatacca aaacaacata gagtaatgta tggaatattg gtaggtatga
     2941 tttctgaaag ttttactata ttacttatat acttaatatc atatccacat tctttagcaa
     3001 ttcaaataat tggtggaata tatttacctt taatagttgg gcagattgga ataggatttg
     3061 ttatatctat agtagagggg attgagaaag ataaaaagga tatagaagcg agaaataaag
     3121 cagaaataaa agcacttcaa agacaaatta atccgcattt tttgtttaat tccctaaaca
     3181 caatagcttc atttataaga tttaatccag ataaggctag agagttaata ataaatttat
     3241 caacttatct aagatataat cttgaatata gtgataactt aattgatata aataaagaga
     3301 ttgaacaagt taaatctttt gtagagatag aaaaagctag gtttggagaa ttactaactg
     3361 tatcttatga tatagaagat gtaaacataa agattccaag tttgattata caaccattag
     3421 tggaaaatgc aataatacat ggtattttag aaagtggtag agctggtgta gtgaaaatat
     3481 ccataaaaaa gttaccgtat tcattagaaa atactgtgag aatatcaata gaggataatg
     3541 gtataggtat aagtgaggaa attataaata atgtatatca agataatatg ccagaaaata
     3601 agattgggct ttataatgta catttaaggc ttaaactgat gtatggaaga gggcttaata
     3661 taagaagaat agatactggg actttaatag tattttatgt aaaggagtag aagtatgaga
     3721 gctataatag tggaagatga atttccagca agaaaggaat tgagatattt tattgaaaat
     3781 aaaagtggca tcgaagtagt aagtgaattt accaatggta tagaggtgtt agactttatt
     3841 caggaaaata aaatagatgt aattttttta gatataaata tacctcatct tgatggaatg
     3901 ctacttgcaa aaacattaaa tcaatttaaa tcaagaccta aaattgtttt tataacagca
     3961 tatgaaaatt atgcagtaga tgctttttct ttggatgtat ttgattatat attgaaacca
     4021 tattctgaag agagaattat ttctatgctg aataaattag aaaaaagtga gatgagtgat
     4081 attgaattat ctaatggaaa tagtaattta tacaaatata agaaagaagc agtaaatcag
     4141 gagatagaag aaataacaca taaaattagc ctttggaaag gtgataaatt agttgtaata
     4201 gatattgatg atatatatta ctgtgaagct aatgaaaggc aaacatttat atatactgag
     4261 aaagaaaagt ttatattaaa agaaggcata tcagaagttg aaaatcttat aaatgataaa
     4321 acatttttta gaacacatag gtcttatatc gtaaatttaa ccaaagtaaa agaaattata
     4381 ccatggttta ataatacata catattaaaa ttaaaaaata gtgactatga agtaactgta
     4441 agtagaagca aagtaaaaga ctttaggctg ttaatgcata tataaagtca tttcatgtac
     4501 ttaaatatgc acttaatgta attaatattt atattagcat aaaaaagtat tactatttga
     4561 actggaaata gttttcttaa actcataaaa atttataaag caattatata aaaactaagg
     4621 ggatgaattt atggtaagct ttttaggttc aatagtagta cttatagtag gttattttgt
     4681 ctatggtact tttgtagaaa aggtttttgg aatcaatgac aaaaatcaaa ctccagctat
     4741 agcttgtagg gatggagtag actatgtgcc aatgaaatgg aagagaatct ttttaattca
     4801 gtttctaaat atagcagggt taggtccgat atttggagct atacaagggg cattatttgg
     4861 accatcagca tttttatgga ttgtgtttgg aacaatcttt gcgggtggag tacacgattt
     4921 tgcttcagga tatctatcaa tgaagaacaa aggtacatct gcatcagagt tggtaggtct
     4981 ttatctagga aatggtgcaa aaatagcaat gagaatattt agtgttattc ttttagtatt
     5041 ggttggagtt gtatttgtaa caggtccagc aggattatta aagacattga caggtgtaaa
     5101 cgcacaaata tgggttggag tgataatact ttattatatt atggctactg ttttaccaat
     5161 agataaattg attggtaaaa tatatccttt atttggagca gcattattaa ttatggcagt
     5221 aggtgtagct ggaggattaa taataaaggg gtataacata ccaaatataa atttacaaaa
     5281 tatgaatcca aatggaacac ctttattccc atacttattt ataacaatag catgtggagc
     5341 aatatcaggt ttccatgcaa ctcaatcacc acttatggcg agatgtgttg agaaagagag
     5401 tgaggcaaga ccagtatttt atggttcaat ggttgcagaa ggtataatag ctttagtttg
     5461 ggcagcagca gccatgtcat atttccatgg tatacctcaa cttaatgtga tatttaatga
     5521 tggtggagca gcaacagtag taaatacagt atctgtcgga ttaatgggtc ctattggagg
     5581 agctttagct atacttggag ttgtagcatg tcctataact tctggagata cagcatttag
     5641 aagtgctagg ctaacaatag cagatgcaat gaattataat caagatgcag tcaaaaatag
     5701 atttttaata gctttaccat tgttcgcagt tggtgttgct cttacattta tagattttaa
     5761 tataatttgg agatatttct cttgggcaaa tcaaacactt gcaatgataa tgttgtggac
     5821 tggttctgca tatttagtca aagcaaataa aaatcattat ataactacat taccagcact
     5881 ttttatgact gtagtaacat ttagttatat aatgcaagca aaagaaggct ttagactacc
     5941 agttaatata tctaatggaa taggtattat tgtagcaata attcttggta ttttattctt
     6001 taaaaaagca aagcatatta aggaacaaaa taatcataaa ttggcaagtt agtaaaataa
     6061 taactataaa aattaaaaaa ttatatttaa attatgttca aa
//
LOCUS       DAEMAE010000210         6094 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEMAE010000210 DAEMAE010000000
VERSION     DAEMAE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.212"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(302..1183)
                     /locus_tag="KPH93_003127"
     CDS             complement(302..1183)
                     /locus_tag="KPH93_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG1689900.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLDAND"
     gene            1639..2613
                     /locus_tag="KPH93_003128"
     CDS             1639..2613
                     /locus_tag="KPH93_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689901.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVISSHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIYEVQKKLQKE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     NIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2729..3775)
                     /locus_tag="KPH93_003129"
     CDS             complement(2729..3775)
                     /locus_tag="KPH93_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG1689902.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDVIYKSLSHPLPFDKTAYPTVGAVAQTPKKPAPPGKHTMLPHQEGS
                     KVVGNFGKNGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAVIGFMN
                     TPHGMFALAPAGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEK
                     ANTGLGQIMYVRYE"
     gene            complement(4386..5237)
                     /locus_tag="KPH93_003130"
     CDS             complement(4386..5237)
                     /locus_tag="KPH93_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1689903.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNAEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNIDNA"
     gene            complement(5254..5391)
                     /locus_tag="KPH93_003131"
     CDS             complement(5254..5391)
                     /locus_tag="KPH93_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689904.1"
                     /translation="MFKSGDLILESLKKYLSKARFDIVRPVLTPDLGACLLAKKKYYS
                     K"
     gene            complement(5599..>6094)
                     /locus_tag="KPH93_003132"
     CDS             complement(5599..>6094)
                     /locus_tag="KPH93_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1689905.1"
                     /translation="SLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFL
                     QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVPWSEFIRQLEYKANWYGRQIV
                     KVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLIT
                     IQNK"
BASE COUNT         1960 a         1018 c          744 g         2372 t
ORIGIN      
        1 tataattaat atacttttta atcaaattta aacagaataa ttataccaat atataaaaaa
       61 atttctaaaa agcaataaaa ttaagttata agcactaata tatttctatt attttaataa
      121 gtatacaatt agattagaat ctaacgctag taataaaaaa attactttcc caaaatagat
      181 ataagcctca ttttatcgtt caaatgatga aaatatattt gatttcatga aaaaagagtg
      241 cctaatgaac taaaaagttc attttgagac actcattttt ttatataaat ttatatagtt
      301 tttaatcgtt cgcatctaac cactcagtta agacttttat atatttctca ttttcttcaa
      361 cgaatggcat atgtcttgaa tactcaaata actcccactt actattaggt attctatcat
      421 acatagtctt tgcaatatat ggtgaacata aatctattgc tccactagtt actagacaag
      481 gctcttttat ttcatgtaat ctatctgtaa attcatatcc agaaagagtt cctgtaggag
      541 aaaattcatt ttgtccccat cctacaatat atgcttcaga acctgatttt ttaggtcttc
      601 tcaggcactc tggagaatct tctgtcactt cacctgcaca atacatttcc ataaatcttt
      661 ctaatgcatc attatattct ttgctagagt aatctccagt atttactgca tcaagtaatg
      721 ctttttggtc agcctcactc atatatgata ttcttctttt ttgttctttc tcccaaagtt
      781 ttgcagaaga aagagtagat gaaaggatgt aagacttaat accttttggt ttatattcaa
      841 tagcatacca aatagcctgc atacctcccc aagattgacc taacagatga atttcatcaa
      901 gtcctagatg ttttcttagt tcaataagct cctctatcca agtatctgca ttaaataact
      961 caggatgccc ttctacaaat gaatttccac aaccaatttg gtcatacatt attacttgtc
     1021 taccactttc agctatttta tccaatactt caaaatagtt gtgtgtagaa cctggacctc
     1081 cgtgaagtaa tactaatggc ttcttccctt ctgtattttc tcctactata cggtaatacg
     1141 ttttaaaacc tttaaaaggc atatatcctt cagtaatttt catgtttatc tcctccaatt
     1201 tgtaattata aacaataatc aagttcatta acaatgctat ttaaaacttc ttatacatat
     1261 ttgattatgt attttaatct ttatacctac ttttataaat agtataacat aaaagttaaa
     1321 aatctctata ctaaaccaat tataaaatca tattaactat ataattttat tttttaataa
     1381 tagtatatac atatttgatt attttcattt gctacttaaa agcaatttta agttttcttc
     1441 ctataataaa cttcctcttt atggtctcat gttagtctct aaatttaaaa ttttacaaaa
     1501 aattttcata aaataaaatt atattgattg tatctaaaca aatagggcaa taaaataatt
     1561 aagtatagtt acttaccttt caagcaatat tagttttatg ttagaatttt attatattga
     1621 tttacaggag gtttacattt gaataatatt aattcactta aagtaataaa attatctctt
     1681 ggatgttctc ttgctatatt tattgcttgg ctattaaaat tagaatactc aatggttgct
     1741 ggagttatag tactacttac agtaaaggac actaaaagag aaactcttaa aggctccatt
     1801 ggaaagattt atggttttct tttatgtact attttttcat atttatgttt taatatttta
     1861 ggatataatt tgacttcatt ttcaatattt atatttataa ttataccact ttgttttttg
     1921 ttaaatattc aagatgttat tgctatgtgt gtagtcatat catcccatta ctaccttcaa
     1981 ggagaaactt ctatcaaatg gattttaaat gaagctggaa tatttgcagt aggaactgga
     2041 ataggtgtct taataaatat gtatattcca acaaatatac ataaaatcta tgaagttcaa
     2101 aaaaaattac agaaagaagt gagtattgta cttatagaca ttgcagatat tattgtaaat
     2161 cctaaaaaag aaaatggata tagtagagat ttatatacat taaattcact tattgacagc
     2221 agtatttctg aaacttacga taatattaat aatactttat taagtgatac tagattcttc
     2281 ttagagcata tgaatattat aaaaagccaa cgggatatct tagaaaattt gtatagttat
     2341 gtatctcaac taaattctac accaccacaa gctcatattc tatctgcttt tatacataag
     2401 ataggttata ctgaatttga agctgaaaca ggcaatcttc ttcttgaaga attaaagaga
     2461 ctaatgctta gtatgaaaaa ccagcctcta cctgttgaca gaacagagtt tgaaaataga
     2521 gctattttat tcctatgctt gactgagtta aaacagtttt tagttaatcg taaacatgct
     2581 cagatgctta gagataataa tttttataaa taaaaagggg agaaatccag cagttgatct
     2641 gactacaaaa tatcagatat aatattaaat gcgcccctag taagagggcg caatgtttat
     2701 aacttattct acggtcatga ttaaatcatt attcatatcg aacatacatt atttgaccaa
     2761 gtccagtatt agctttttca tcagtgattt ctattttaat agtcacatca ttttttttgc
     2821 agtacttcgc taattctgca ccagaacgat ttttaccact aaaatctaaa gaaaatggct
     2881 tatcatattt aaaaggatat tcaacaggtt tacccgctgg tgctaatgca aacatcccat
     2941 gtggtgtgtt cataaatcct attacagcaa tcgtaagatt tgatgggtct aaatttgatt
     3001 cactacctgt aaatgtaatt gtatttactg atgaattaac tacactacat cgaacacctt
     3061 catacattac atgtttttca tttatatctt ctccattttt tccaaaattt ccaacaacct
     3121 ttgacccttc ttgatgtggt aacatagtgt gcttcccagg tggtgctggt ttttttggtg
     3181 tttgtgctac tgctccaact gttggatatg ctgtcttatc aaatggtaat gggtggctaa
     3241 gactcttgta tatgacatcc tttcctgctg atttattagc atcaatatct acatgataca
     3301 aacgcccatc accaaaacgg ataagattcc actgatggtc atttgatgtg ctgtggacat
     3361 aataagattc aattcccata gcgtcacaca aagctttcat gctcctagcc attcctgaac
     3421 atgcaccttt tttagtaacc aatgtatagg cagtattcgc tccttctact gcattccagt
     3481 caacatatgt tgtgttattt ttaatgtgat tataggcaac taaaatctta gcatctgtag
     3541 tcatattctt tgtgatttta ttgttcataa aatctgctgt tgcttgtgct actgagttta
     3601 gttgggcttg tgacagcttt ggaccatata cgctattggt tccagtgaca tagctttttg
     3661 gtgtttttgc tgttacctct ttggctgtca taccataaac tgtcatactc atacctgaga
     3721 ctaaaacaat tgttgtaatc aataacttgg tagctaactt cctaattttc ttcatactta
     3781 gtattctcct ttataaaata ttgttacttc ctattctcta ccattaataa agttcatttg
     3841 ggatttaaaa aggtggattg tcaatatgta tgtttcttca tttatacgtt cgtgaaatgt
     3901 ttaaatttca tggaaaatct aatagatatt ttctagataa tttgtataca atacaactcg
     3961 caaatcagta tgtttgctgt tagtttgaga aaatgatgat gattaatata tgcggaatct
     4021 ttgacctata ataaatacta ctgtcttcaa tcattaataa tggtaagagt aaagagaatt
     4081 atcaataata cagttaaaat caactttgta tacatttcat acattctctc tccacaacat
     4141 tatacctcct ataatttgta tattcaatac attttttagg aaaatctagg atatatctgt
     4201 aagaatataa aaaaggagtt gagcttaaaa tttgattaaa aaaattaatt ttaagacaac
     4261 tcatttgtat tataaaaatg ctatatatta ttattgagta agtcaatatt ttagatattt
     4321 atctatttct tattgactta cgcttatagt ctaacttatt tgtttagttg atatataatc
     4381 tagttttaag cattatcaat atttaaataa tcaagtatcc tttttgtttc ttgtagtttc
     4441 tgttctgcct catttaagtt ttcttgaatt atagaaatat atattatatc tatcaacgta
     4501 agcattgcta ttcttgaaga aatagctcct tctctaagcc ttttttctat atttggtact
     4561 actagcttta aatcactaat cttactaaga tgattgtcac tatatttagt tatacaaatg
     4621 accttacatc cttttaattt tgcattttct atagacttta ttacttcttt tgattctcca
     4681 gagtaagata ttgctatagc tatatcttct ttatctacat ttgaagatac cataagttgt
     4741 gtatgactat ctaaagaggt aaatgcctgt ttatttatcc ttaaaagttt catctgtaaa
     4801 tctagtgcaa ctaaagccga accacctact ccaaatatat atacattttt agcctttatt
     4861 atctgttcaa ctgcttcttt tattatatct ttttcattca acaaccttgt ttgattgata
     4921 gtctctatgt ttttgtttgt aattttgttt ataacactat ctatattaca atcatagttt
     4981 tcatctacat atgtgtattc attttctttg cccatcaaat ctttagctat ctcaattttt
     5041 aaatccccaa aacccgaata tcccattttt ctagaaaacc taactatact tgctgtactc
     5101 gtttttgagt ttgatgccaa ttctgataca gaaagcttac ttatagcctc tgcattatta
     5161 agtatgtaat cagctatttt tttatctact tccgttaaat cttcatatat ctgttttaat
     5221 tttaaaatgc aactcatacc ctaccacctc tacctattta ctataatatt tcttcttagc
     5281 aagtaaacat gctcctaaat ctggagtcaa cactggtctc actatatcaa atctcgcttt
     5341 actcaaatat tttttaagtg attctaatat caaatctcct gacttaaaca ctccacctga
     5401 ataagatacc tttatctttg taccttgttc aaaatgatgc tctaatccta ttataagcct
     5461 acttaattca taagctgcat cctgaacctc ccatgactaa agtcacaggg ttcctgcttc
     5521 atagaatttt gcatactaaa atatgtctta ctaattctcc acaggctatc cccgtagttc
     5581 ctacggttct tacataagtt atttattttg aattgttatt agtcttaatc cttcttttaa
     5641 tatatttata cttgcattta tatctctatc gtgagtttca ttacaactag gacaaatcca
     5701 ttctcttata tttaagttct tcacttcctc gtttttgtac ccacatttat tgcatatttg
     5761 tgaacttgca aagaatttac ccacctttac aatttgtcta ccataccaat tagccttgta
     5821 ttctaattga cgaataaact ctgaccatgg cacatcagaa attaagcgag atagtttacg
     5881 atttctaatc atattcttta cttgtaaatc ttctatgcaa ataatatcat tctcttttat
     5941 taatttagta gatagttttt gtagaaaatc atttctttga ttagctatat gttcttgaag
     6001 tcttgcaact tttaatcttg ctttatttct atttaaacta ccgattgttt ttctcgataa
     6061 ttctctttgt aatttagcaa gtttgtttaa tgac
//
LOCUS       DAEMAE010000211         6069 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEMAE010000211 DAEMAE010000000
VERSION     DAEMAE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.213"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..486
                     /locus_tag="KPH93_003133"
     CDS             136..486
                     /locus_tag="KPH93_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG1689906.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            505..2148
                     /locus_tag="KPH93_003134"
     CDS             505..2148
                     /locus_tag="KPH93_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG1689907.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKDLVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNADLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKDINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2229..4286
                     /gene="recG"
                     /locus_tag="KPH93_003135"
     CDS             2229..4286
                     /gene="recG"
                     /locus_tag="KPH93_003135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG1689908.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKDLIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMGLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4345..4899
                     /gene="rsmD"
                     /locus_tag="KPH93_003136"
     CDS             4345..4899
                     /gene="rsmD"
                     /locus_tag="KPH93_003136"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG1689909.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4901..5398
                     /gene="coaD"
                     /locus_tag="KPH93_003137"
     CDS             4901..5398
                     /gene="coaD"
                     /locus_tag="KPH93_003137"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG1689910.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5402..5926
                     /locus_tag="KPH93_003138"
     CDS             5402..5926
                     /locus_tag="KPH93_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689911.1"
                     /translation="MKIDLEMIELFEQLEDIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
BASE COUNT         2477 a          631 c         1167 g         1794 t
ORIGIN      
        1 aaaaactttt ctaggtttaa attataccat atttatagta tgatatgatt ataattttat
       61 ataataaata agtaaaaata gtaaaaaata aattaagaaa tgtttttaat aaaaataagc
      121 ttaagggggt caaccatgag tgcaaaggta acgaatcaat atggaaatat agaaatagat
      181 aatcaagtaa tagctcaagt cgtatataga gctgcaatgg aaagttatgg cttagtaggt
      241 ttagcatata agtctaaagg aatagtagag ctattaaaag gcgagaacgc aactaaaggt
      301 gtaagagttg aggaagtaga agaagatgcc atagcaatcg aactttatgt tataatacaa
      361 tatggtacaa atatatctac tgttgccaat aatataatag atagagtaaa atatgtagtt
      421 gaaaaaatga ctggcgtaag agttactaga attgatatta atgtacaagg aataagagtt
      481 aagtaatttc ataggaggaa acaaatgatt cagtatatag atggtaaaag gttaagggag
      541 atgtttattt caggtgcaaa caatctccaa aacaataaag atttagttga caagttaaat
      601 gtattccctg taccagatgg agatacagga actaatatgt cattaacaat atcttatgcc
      661 ttaaaagaat tagcaaaagt tgaaaatgat aatatatcag atatcggaaa agcattatct
      721 aaaggctctt taatgggagc tagaggaaac tcaggagtta tactttcaca aataatcaga
      781 ggtatagcta aatctataga aggaaaaagt aaattatcaa cagaagattt agcaaaagct
      841 tttaaaaatg gttcagacac agcatataaa gctgtaataa aaccaattga aggaactata
      901 cttactgtag ttagagagag tggagagttt gcaataaaaa ctgcgaaaaa agagaaagat
      961 gtagtaaagt ttttaagtat gcttgtgaag gaatccaatt cttcattaga aagaactcca
     1021 gaccttctaa agaatctaaa agaagctggt gtggttgact ctggtggaaa gggtcttgta
     1081 cttatatatg aaggaatgtt agcttctata aaaggcgata acatagaaat aaaaaatgct
     1141 gacctagata ctaatatttc aacaagtatg gattttgcaa agagtactac tagcacagat
     1201 gatataaaat attgctattg tacagaattt attttagaaa gttctaaggt agaagatacc
     1261 aaaataagag acataatgat ggcttatggt gatagcttag cagtagttgg agatgatgga
     1321 gtcataaagg tacatgtaca cactaatgac ccaggaaatg tacttcaaga agctctaaag
     1381 tacggtcaat tattaactat aaaaatagaa aatatgaagt tacaacatga aaatacatta
     1441 ctggatgtag aagaaaagaa agaaaatgat agtgaacctt tagaagaaga aaaagagttt
     1501 gggtttatag ctacttctat gggtgaaggt ttggcaaata tatttaaaga tttcggagtt
     1561 gaccatataa tagaaggtgg tcaaactatg aatccaagta ctgaagactt tatgaatgct
     1621 ataaaggata taaatgctaa aaatatattt attttcccaa ataacagcaa tataataatg
     1681 gctgctaatc aggcaaaaga attgagtgat aaaaatataa tagttatacc tacaaaaaat
     1741 acgcctcaag gatttgctgc attagtaact tttaatggag aattaagcga agatgaaaat
     1801 aaagaagcta tgatgaatgc tttaaactca gttaaatcag gtcaagtaac ttttgctgtt
     1861 agggatacag tgatgaatga aattgatgtt aaagaaggta atataattgg aattgcagaa
     1921 ggaaatttat tatcagcagg agattatgtg gatgaagtaa catcaaacct tattgaaaag
     1981 ttggttgatg aagatactgc tataataaca ttattctttg gtgaagatgt tacagaatct
     2041 caagcaaatg aactacgtac ttctttagaa gaaaagtttg aagatgtaga tgtagagtta
     2101 tattatggag gacaacctct ttattattac ttaatttcag ttgaataatg attataagaa
     2161 cctgtatgac caagtagctt ttataaaatt ggttgtacag gatttttgtg tagtaggtga
     2221 tatatttgtt ggatttatat aaagatgttc aatatgttaa gggaatagga cctaaaaaag
     2281 cagataagct aaataagctg ggtatattta cactaaaaga cctattgtat tattttccaa
     2341 ggcaatttga agatagaaat aatctaaaga aaatagcaca attagaagat ggtgagaaag
     2401 ttaccataaa agcagtaata tcaagtataa atacatttag cccaaaagaa ggtatgactt
     2461 taacaaaaat tgatgtaaaa gatgagacag gctcggctaa attagtgttt tttaataaga
     2521 gttatataaa aaatactttt cgtccaggag attcaatatt agtttttggg aaggttaaga
     2581 aaaaatttaa taacttagaa cttacatcat gtgaattaga atatcttact aattcaccta
     2641 agaatacatg tagatttatg cctgtgtacc agcttacata tggtgttaca aataaagaaa
     2701 ttatgagtat aattagaaca gtacttgaag ataaagactt aattatacaa gaatatatgc
     2761 cacaaagaat tatagaaaaa tataggctat gtagtataga ttttgcagtt agaaacatac
     2821 attcaccaag tagtaaggaa tcattaaaaa tagctttata tagaattgta tttgaagaat
     2881 tacttatact acaactaggg ttgtttgtat ttaaaagtgg cagaaataaa gaagatggaa
     2941 taaaatttga aacaagtaaa gatttaaaaa aaataataag tgctttacca tttaaactga
     3001 ctaaagctca aaatagggca ttagatgaaa ttatacaaga tatgaattta gagaaaataa
     3061 tgaacagatt ggtacaaggt gatgtaggga gtggtaaaac agtagtagct ctcttagcac
     3121 ttgcaaattg tgttttaaat ggttatcagg gtgctttgat ggcacctaca gagatattag
     3181 caggtcaaca ttatatttca cttactgagt ctctaaaaga ttttggaata aatgtaggtc
     3241 tactaatagg tagtcttaca aaaaaacaaa aggatacagt attggaacaa ataaagaata
     3301 atgagattga tatattaata ggtacacatg cactaattga agataaggtt gaatttaata
     3361 atattggttt agttataaca gatgaacagc atagatttgg tgttatgcaa aggagcaaac
     3421 tttcattgaa aggagcaaat cctgatatat tagtaatgac agcaacacca ataccaagaa
     3481 ctttagcact tatactttat ggagatttag atatttctat tatagatgaa cttcctccag
     3541 gaagacagcc aatagaaaca attgctattg aaaaaagtaa gagagataga gcatataata
     3601 atcttgtaag aagagaggtt gaatcaggga gacaagtgta cattgtatgt cctttggtag
     3661 aagaaagtga agccatagaa gccaaatcag cagttgaact tgttgaggag ttaagagcag
     3721 aatattttca cgacttaagg cttgggttgc ttcatggtaa aatgaagtca agtgaaaaag
     3781 atgaagtaat gggacttttt aaaaataaag aaatagatat tttagtttca acaacagtaa
     3841 tagaagttgg tgtcaatgtg ccaaatgcaa cattaatgat aatagaaaat gcagaaagat
     3901 ttggacttgc acaattacat cagcttagag ggcgagttgg aagaggaagt cataaatcgt
     3961 actgtgtact aatttatgat tctaaaacag atgtatgtag acaaagaatg gctataatgg
     4021 aagagaccaa tgatggtttt aagatttcag agaaggattt agaaataaga ggacctgggg
     4081 aattttttgg gacaaggcaa catggtcttc cagaacttaa ggtagcaaac ttatttaaac
     4141 atataaaaat actgaagtta gcacagcaag aagctcgata tatattgggt gaagataata
     4201 atctacaatt aaaagaaaac atggcactca aaaaagaaat tatagataaa tttaaagata
     4261 cattgaaaga aatttcatta aattaatttt aaattgtgtt aaaatattac taatgtaata
     4321 atgtgctgaa gggagattgt catattgaga gtaatttcag gaaaagcgag aggattaaaa
     4381 ctaaatactc caaaaaatga agatgtaagg ccaactacag atagagtaaa agagtctcta
     4441 tttaatatga taaattcata tataatggaa agtgaagttc tggatttatt tgctgggact
     4501 ggttctttag gaatagagtg tttatctagg ggtgccaagt catgtacttt tgtagatatc
     4561 agtaaggaaa gcatagatat tgttaagtcc aatataaaaa aggcaagagt tgaaagtgaa
     4621 agtattattt taaacctaga ttttaagact gcaatagata aacttaaatt acaaaatagt
     4681 aaatttgaca taatttttat ggaccctccg tattataaaa atatgtttat agaagctatt
     4741 gaaaaaattg ataattctaa tctattgaac gaagatggaa taatagttgt agaacatgat
     4801 acgaatgatt tatttccaga taagatatgt aaattggaga aaactagaga taagaaatat
     4861 ggaaatacta cattaacttt ttataagatg gaggcataat atggaaaata aacctagaaa
     4921 agctatattt gcaggaagtt ttgacccaat tactaatgga catttagata ttatttgcag
     4981 ggcttcgaag ttatttgacg aattacaaat aggagtatta aataatccta ataaaaaggg
     5041 tctatttagc tttgatgaaa gagtaaaact tatagaaaaa agtacaagcc atttaaataa
     5101 tataaaagtt gtgacttttg atggtttatt aataaattac tgtcaggaaa atgggattgg
     5161 agcattagtt agaggtgtga gaagtggtgc tgatgttgat tatgaacttc agatggcgca
     5221 tatgaataga gagcttaatc cagatataga gactataatt ttaccaagtt gtacaaaata
     5281 ttcatttata agttcatctt taataaaaga ggttttgctc tttgatgctg atataaaaaa
     5341 tttggttcca aagattgttt tggaagaatt aaagaaaaaa actagtgggg gtaattgata
     5401 tatgaagata gatttagaaa tgattgaact atttgagcag ttagaggata tatttagaag
     5461 tgcttctaca atccctttct cacataaatg cacagtggat aaagatgagg ttctagcaat
     5521 aattaacgat ataagaactt taataccaga agaagtaaca caggcagtat ggattaataa
     5581 agaaagaaat aaaataataa gtcaagctaa gcaagatgct acaaatatag ttgaacaagc
     5641 gaaaaaagaa gctgagagaa tacaacaaga atatcaagat aatatagagg aattaaagaa
     5701 aaattctgaa gatgtagtaa aggcatatgt agaatccagt gaacctgttg ttcaagcaga
     5761 ccaaagagca aaagatatag ttgatagagc tgagagaata gccaatgaga taagaattgg
     5821 gtcaattgaa tatgcagagg atgtgctttc tagtgttgag tataatttaa gagaaatatt
     5881 agaagaagta aaaagagata aagctgaact tagacctaaa aaataaattt taaataaata
     5941 aaaatggagc tatcacacaa aaaattccaa ttgatgtgtg atagctctct tttaatatag
     6001 caattttttg ttacctaatt gtgcttttca gattacttat atcttatttt gatattataa
     6061 tgtagttta
//
LOCUS       DAEMAE010000212         6057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEMAE010000212 DAEMAE010000000
VERSION     DAEMAE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.214"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(514..1035)
                     /locus_tag="KPH93_003139"
     CDS             complement(514..1035)
                     /locus_tag="KPH93_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689912.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEIMKNKE
                     YQFNLIYDKNQFVGIILCWETDSFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTVIL
                     EIDLPIDEISIYRKAFYERVKYKTNDYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     NFNQYLRNNVMSF"
     gene            1391..2188
                     /locus_tag="KPH93_003140"
     CDS             1391..2188
                     /locus_tag="KPH93_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG1689913.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            2298..2888
                     /locus_tag="KPH93_003141"
     CDS             2298..2888
                     /locus_tag="KPH93_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG1689914.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGARNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVRADWSCFTDFNIVSRNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2954..4651
                     /locus_tag="KPH93_003142"
     CDS             2954..4651
                     /locus_tag="KPH93_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG1689915.1"
                     /translation="MDYTKTYEEWTKGSYFDEDTKLELEKIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTGANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNIPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKSNNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            4865..5704
                     /locus_tag="KPH93_003143"
     CDS             4865..5704
                     /locus_tag="KPH93_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG1689916.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFLKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLIGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         2389 a          616 c          994 g         2058 t
ORIGIN      
        1 atagttataa tgagcttaga gaagaaccaa tttttaaggg tgtaattgaa aagattttag
       61 agatggaaaa aatgttaaaa aatgaagaat aagtaaaaat ttaatatagg acttaagact
      121 aatcaaatta aaaggaagtg tttcaaaata aaaatttaaa tcaccttttt tgttggtctt
      181 aaacaattat ttagaaaaat agaggtttca aaataaaaat ctgaattatc tgtcttatta
      241 cacaaaaata gaaacctaaa tttgcaaaaa taaaataaag tttgttatat aaaatgtaat
      301 tttgtctata aaatctcaca tgttttctta actttaagtg tgaaaaagag ggtgtctcaa
      361 aatgaacttt ttagttcatg aggcatcctt ctttgtatga aatcgaatat atctttataa
      421 tttaaacaat gaaaaagaat cttatttcta ttttgagaca tctcttttat ttaattatga
      481 aattttacac agttcaataa ataggaatta catttagaaa ctcatgacat tatttcttaa
      541 atactgatta aaattatcat attccatttt agacaatttt tctgggtaag acattacaat
      601 cagattatgt ccattaaatt tctcatggta tggtggatga atgtgttcat aatcatttgt
      661 cttatatttt actctttcat agaacgcctt gcgataaatt gaaatctcat caattggaag
      721 gtcaatttct aaaataacag tttttccttt cttatttaaa agctctaatg ctctttgtcc
      781 atatctatta ttacgcattt ctggaaaaat gcagaaatgt tctacataga taaagctatc
      841 tgtttcccaa cataaaatta tgccgacaaa ctggttttta tcataaatta aattgaattg
      901 atattcttta tttttcataa tttctgcttg caaagaagat tttctttgtt catgaaatgg
      961 aaaactaatt ttatataatt ccatagcctt gtaatataat ttattttcac tggttcttaa
     1021 ccgttcaaat ttcactttac tctccctaat cacgaaagtt ctaatttatt tatgagttca
     1081 ttttaccata gtcattattt gcatacaatg tgttttaagt tgagtgtcat ttctttcttt
     1141 tatttatgtt tatatctcat agactaatgt ataattgctt aagacataac ttaaaactat
     1201 tatattaagc tttgataatc taaaaacagt atatttatgg acttttctag gtctaaaatt
     1261 aatcttaata attttagaag ttacaaaaaa gttacataaa tttggaaagc tataatatac
     1321 atatataata taaggttaaa actgtaatga ataatattaa tttaaaattt gaacatggaa
     1381 ggagattatt atggagaaaa gagaaaagag gataataata tcatctttat taagtgtatc
     1441 aattttaatg ggtttggtaa gtatatattc tatattaaat aaagaagaca taagtttaac
     1501 agttaaaggt caagagcaaa aagtatcttc ctttaagaaa acagttgagg aacttttgga
     1561 tgaacagggt gtaaagtata attctgaaga taagattaat ccaagtttag atacagaact
     1621 aaaagatgat atgaaaataa aagttgttaa ggtaactaaa agtaaaaaag aagagattga
     1681 aaaaattcca tttgatacaa agcatgtaaa tgatagtaat ttgttaaaag gaaaatctaa
     1741 agtttatcaa gaaggtcaag agggagaaaa aaaactagtc tataatttaa cttaccatga
     1801 tggaaagtta gtcaaaaaag tcttatcaaa agaagtaata tctaaggagc caactacaaa
     1861 aattataaaa tatggaacta aagaaaaagt attaatagca tcaagaggag caaatataag
     1921 aggaggcaaa catatgaaag tggttgccac tgcatatgca ggagatacaa taacatctac
     1981 tggtacaact ccaagatggg gtgttattgc agttgaccca cgtgtaatac catatggaac
     2041 aaaagtatat atacctaagt tgggtatgac ttttgtagca gaagattgtg gaggtgcaat
     2101 taaaggtaat agaatagata tttttatgaa cagtgaagga aaagcctcaa attggggaag
     2161 gaaaagtata gatatatatc tacattaaat tagtgaatag aataacttta cttgaaggac
     2221 aaaaaagtat aaaatatgaa ttaactaaac tgttagatta ttcttaaaac ataaaaatat
     2281 atctaagggg ggataagatg aaaatagtct gcttaggaga cagtttaaca tatggatttg
     2341 gagtatcacg aagtaattca tggacaaaca ttgtaaataa agagactcga ttagaaatag
     2401 taaataaggg tataaatggt gatacaacaa gtggtatgtt agctaggttt aatgaagatg
     2461 ttgtaaaaaa ctctcctgat atagttttta taatgggtgg caccaatgat tttattgctg
     2521 gtgcgagaaa tgaaattatt aattctaata taatggctat ggtgcatcag tcatattcaa
     2581 aaaatatcat acctataata ggtataccat taaaaccaga tattccaaat gttagggcag
     2641 attggagttg ttttactgat tttaatatag tatctcgaaa cttagaatca tatagttatt
     2701 ggataaaaaa attttgtatg acatttaata ctaatttcgt agatttttat tcagaatata
     2761 ataaaaatat ggaactagaa ggatataaaa aattatattt tgatgggtta catccaacca
     2821 aggaaggtca tagaataatg gcagacattt ttattaattc aattaataag tatatagaag
     2881 agttatagat tacattaaaa tgcttgttta tgtgtcagtt gcaatgaata catacagtag
     2941 agaggggttt attatggatt atactaaaac ttatgaagag tggacaaagg gttcatattt
     3001 tgatgaagat acaaagctag agttggagaa gataaaaaat aatgaaaaag agatagagga
     3061 tagattttat aaggatttag aatttgggac tgcaggtctt agaggaataa ttgaagcagg
     3121 aacgaataga ataaataagt acacagttag aagagctact tttggattgg caaactatat
     3181 attagaaaac acaacaaaag aagaaacaag cagaggagta gtaatagctc atgataatag
     3241 acacaagtct agacaatttt gtatagaatc tgcaaatact ttggcagcat gtggtataaa
     3301 agcatatata tttgatagtt tgagaactac tccagaatta tcttttgctg ttagaagctt
     3361 aaatgcaatt gctggtatag tcataacagc aagccataat cctccagaat ataatggata
     3421 taaggtatac tgggaagatg gagcacaagt tatgccagag attgcaaatg caataacaga
     3481 gaaagtaaat agtatacatg attatagtac aataccaacc ttaacagggg ccaataaaaa
     3541 tttggtggtt ttgttggatg aatcacaaga tactaaattt atagaagcag taaaaagcca
     3601 aattataaga aaagatttag ttaaaaatgt aggtaaaagc tttaagatag tctatacgcc
     3661 tctttgtgga acagggaata ttccaataag aagagcattg aaagaagttg gttttgaaaa
     3721 tataattgta gttccagaag aagaaaatcc agacccaaat tttgcagggc ttgattatcc
     3781 aaatccagaa gagaaaaaag ctttaaatag aggtatatta ctcgcaaaag aaaaaggagc
     3841 agaccttgta atagcaactg acccagattg tgatagagta ggtgtagcag taaaaactac
     3901 tacaggtgag tatgctttac ttacaggaaa tcaaattggt ggtatgctga cacattatat
     3961 aatagaagga ttaaaagaaa ataataagtt aaaagaaaat cctacaataa taaaaaccat
     4021 agttacgtca gaatttggag cagatatagc taaatcaaat aatgttgatg tattaaatgt
     4081 tcttacaggc tttaaattta ttggtgaaaa aataaaatta tttgaacaaa ataaaaatag
     4141 aagttatgta tttggatatg aagaaagtta tggttactta gttgggactc atgctagaga
     4201 taaagatggt gtagtttctt cattgttaat atctgaaatg gcagcatttt actattcaaa
     4261 aggtatgagt ttatatgatg gtttaataga actttataaa aagtatggat ttttcaaaga
     4321 acagactata tctttgactt taaaaggtat agaaggtgta gaaaagataa aagaaataat
     4381 atcttacttt agagaaaatc aaattgatta tataaatagt attaaagtag ttgataaaaa
     4441 agattataaa aatggtatag ataatcttcc taaatctgat gttttaaaat actttttgga
     4501 agatgaatct tgggtagcta taagaccatc tggaacagaa ccaaaactta aattttacat
     4561 agctgtaaaa ggagctagtg atattgaagc agataaaaag attcaagggt tgaaaaaata
     4621 tatagatgac atggtcgaaa aattaaaata aaatattatt attttataag agaattttgt
     4681 aataatattt atttagctga aaattataat ttactttcat tgagtagaat atggaaatgt
     4741 tagtagaatt attattttca gcttttattt gtgttatttt tacttaaata attatattat
     4801 agaatgaatc tgagtataat taatattgta taatgaaatt aatttgatag aaaaggggat
     4861 atttatgttt ggtatagata agaaattgat tgaatatata attgcaaaat ccaattacag
     4921 tgaattaagt tcaaagtcag cagaagtatc tttcttctta atgttgtcta tatttccttt
     4981 tttgatattt acaataagtt ctatagctta tataccaatt cttcatttaa ataagtatat
     5041 agctttgttt agaaatatga tgccagaagg tgcttttgct gtgttatctt caattatagt
     5101 ttctgcaata gacaatagaa acctgaaatt tttagcagtc agttttgtac ttactatgtg
     5161 gacattttca agagcagtaa aagctcttat taaaggaatg aatagagctt ataaagttaa
     5221 agaaactagg tcttttttaa aaattttatc tatatcattt ttatttacaa taatgctttt
     5281 ggtacttatt tttttatcta tgatatttct agtttatgga gaaaaaatag gttattttat
     5341 ttttaacctt ataggattgg atgaaatatt tataaaaata tgggatattt taagatacac
     5401 tgtaggtata ataacaataa tagtgatatt tacactttta tataaatata ctccaaataa
     5461 aaagttgact attaaagaat ctgcgccagg tgccatcttt gctacttttg cttggttttt
     5521 agtatcgttt ttatattctt attatacaaa ttactatgct aactatgaag taatctacgg
     5581 aagcatagcc gaaataattg tacttatgac ctggatgtat tttagtagtt ggtctatcgt
     5641 tattggctat gaggtaaatt caagattgta ttttagaaag ataagacatg aaatgttaaa
     5701 gtaaagtatt aaaataaaag ataggattta atattataaa tgtaattgaa ttaataaaga
     5761 aatattgaca tttaagttaa aaagtctaga atttttctgg gcttttttta tttttagtat
     5821 aacttgacaa actttatttt atgagataat tttaatttat aatcatcttt gaaaagatga
     5881 ctattgataa gtaagagata aaattggtag aagcatcaaa aaatatacta aataaaaatt
     5941 atagtcctaa tgagttaatg tctgtatttt caaataatgc agtatttagt catgtgtttt
     6001 tatatgcgcc tgtgttccta attaccaata tattagaata agctactgta attagat
//
LOCUS       DAEMAE010000213         6033 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEMAE010000213 DAEMAE010000000
VERSION     DAEMAE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.215"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            324..1013
                     /locus_tag="KPH93_003144"
     CDS             324..1013
                     /locus_tag="KPH93_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689917.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVALQKKTGKSTNLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNTVK"
     gene            1448..1987
                     /locus_tag="KPH93_003145"
     CDS             1448..1987
                     /locus_tag="KPH93_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1689918.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            2006..3049
                     /locus_tag="KPH93_003146"
     CDS             2006..3049
                     /locus_tag="KPH93_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689919.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKESN"
     gene            3051..3887
                     /locus_tag="KPH93_003147"
     CDS             3051..3887
                     /locus_tag="KPH93_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689920.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            3992..4666
                     /locus_tag="KPH93_003148"
     CDS             3992..4666
                     /locus_tag="KPH93_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689921.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGTK"
     gene            4666..5718
                     /locus_tag="KPH93_003149"
     CDS             4666..5718
                     /locus_tag="KPH93_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1689922.1"
                     /translation="MIKFKKVISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWDILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
BASE COUNT         2301 a          607 c         1103 g         2022 t
ORIGIN      
        1 attaataata cataataaag taataaaaaa ctgtttaagt ataataactt atatttagac
       61 agtttttttc atttataaat aatataaatg aaattaaaaa aatttaaagg atagaaattg
      121 gttgaataaa tgtataaatt atttcatcga aaaaataatg acaaaaattg taactaaatt
      181 gtaataaaat ggtagttatt tagcataaaa taatgtatca tagaagtata ttataaaatt
      241 aataaaatag gatttttata agaaaactgc tggctgttga tatttaaagt gagtagttaa
      301 attatgttta ggaggggtag aatttgttac aaaaagtatt atggaaattt ttaaagctag
      361 tagtcggttt atttatatgt tcagttggta tagtattgac gataaattgt aaccttggat
      421 tatctccctg ggatgtattt catcaagggc tttcaaatca tataggaata actataggaa
      481 ctgcttctat aattgtaggc tctatagtag ttattgctga tgtcgttctt ggagaaaatg
      541 taggatgggg aactgttttt aacatgttac taattgggtt ttttatggat ttacttttat
      601 atagtaattt aattccagag gctgatagtt tatttgtagg aataattatg ttgatattag
      661 gattggttct tttgtctgtt ggaatggtat tttatatggg aagtggttta ggaagtggac
      721 ctagagatgg tttgatggtc gcacttcaaa agaaaactgg aaaatcaact aatttaataa
      781 gaggtactat agaagttgga gcactaatag taggattttt attaggtgga aaagttggta
      841 taggaactat aataagtgca tttggactgg ggtattttac acaaatggta tttggcttat
      901 ttaaattaga ttgtagtaag attaaacata gatttatagt agatgatata aaatttatca
      961 aagtgtatat gagtggtgag aaaaaagctg ctgttcaaaa tacagttaag taatttaaga
     1021 ataaattcaa cgaaatgtta agtattttaa attaacattt tgtttagatg gataaattga
     1081 atattaaaag aacattaaaa tatgaggatt gctagaacac tataaaatat agatttctag
     1141 caatttttta tttgttcaat gtttttttag tattagaagt cttattctct atttaatgaa
     1201 tgaattaatt tataattaga gatactaaac tttatagata ttaatataac cacctttaaa
     1261 ctgtgtattt tccaaagcct ttaaaatgct tgtatttatt ctaaataaaa aaataaaaaa
     1321 atgtcaaaaa actcttgtat tatatccaaa ttttatatat aattacctat gttgtttaga
     1381 aattttaaac tcaatgttta gtaaacaaaa tgtttagtaa aataaaacag aaactaggag
     1441 gtgcaacatg gatataggtg aaaagataaa aagattaaga acagaaaaac aactgaccca
     1501 ggaagaatta gctaatagat gtgaattgtc aaagggattt atttctcaat tagaaaataa
     1561 cttaacatca ccatccatag ctacccttat agatatactt gaaatactag gtacaaattt
     1621 aagggaattt tttaatgaaa tagatgatga aagaatcagt tttacaaaag aagatatgtt
     1681 tgaaactgaa gatgaagatt taaaatataa attaaagtgg ctaattccaa attcacaaaa
     1741 aaatgaaatg gagccaataa taataacatt gtatccaggg ggacagtata aagaagaaaa
     1801 gccacatgaa ggtgaagaat ttggatatgt attagcaggt tcaatatatg tccatatagg
     1861 cgaaaagaaa aataaagtaa aaaaaggaga gagtttttac tttagaccga aagcaaatca
     1921 ctatatatca aacgaaggca aaactacagc aaaagtgatt tgggtaagta cgccaccatc
     1981 attttagaat agaggtgttt aataattggt agaaaatata atagaattaa gggatatatc
     2041 taaacattat gaggaactta ctgttttaga taatttcaat ttagatataa agaaaaatga
     2101 gtttcttaca ttacttggac caagtggatg tggaaaaact acaacattaa aaataatagc
     2161 aggatttgaa tatgctgatg atggtaaagt tttatttgaa ggtaaggaga taaataattt
     2221 gccaccatat gaaagacaag taaacacagt atttcaaaaa tatgctttat ttccacacat
     2281 ggatgtttat gaaaatgtag cttttggact aaaaattaaa aagactccaa aagatgtaat
     2341 agatgctaaa gtaaaagagg ttttgaaact tgttgcacta gaaggttttg aaagacgaca
     2401 agttgaatca ttgagtggag gacaacaaca gagaattgca atagcaagag ctctagtcaa
     2461 tgaaccaaag gtgttgttac tagatgaacc acttggagca cttgatttaa agttaagaca
     2521 agagatgcaa atagaattaa agagaatcca aaaaaaatta ggaataacat ttgtttttgt
     2581 aactcatgac caagaagagg ctcttactat gtcagatact attgttgtaa tgtataaggg
     2641 taaaattcaa caaatgggaa caccacaaga tatatacaat gaacctaaaa attcatttgt
     2701 agctaaattt ataggtgaaa gtaacatatt tgatggaatt atgatagaag attataaagt
     2761 taatttctgt aatagagatt ttgagtgtgt tgataaagga ttcgaaaaaa acgaaaatat
     2821 agaagtagta ataagaccag aagatataaa aatggtaaag ccagaagaag gaatgttaaa
     2881 ggggaaagtt acatctacag tgtttaaagg agtgcattac gagatagaat tgaatgaaaa
     2941 tggaagattg tggatacttc ataatactaa aaatgctgag gttgggactg aactaggcat
     3001 ggatatatat ccagaagata tccatataat gagaaaagag agtaactaag atgaaaagaa
     3061 aatctttttt agcatatcca tatgtagttt ggagtgcgat atttgtaata attcctttaa
     3121 tactagtggt attttttagt tttacaaaag aatcaggtgg aggttatgcc tttacacttg
     3181 agaattataa agaagtaatt gaccctatat atatgaaagt ttttgggcgt tctattcttt
     3241 tagcaggagg agctacatta atatgcctta tagttggtta tccagtggca tatataattt
     3301 caaaggctcg tgtaagtaga agaggttcat taatattatt atttattctt cctatgtgga
     3361 tgaactttct tttaagaaca tatgcatggg ttgctatatt aggaaaaaat ggacttttaa
     3421 atacattttt aggttggttt ggaatacaac cattagcaat tttgtatact aattttgcta
     3481 tacttttagg tatggtgtat aactttttac catttatggt tcttccaata tatacagcac
     3541 tatcaaagat ggataatgat ttgataaacg ctgctcatga cttgggtgca aataatatga
     3601 cagtttttag gaaaattata tttcctttga gtttaccagg tgtaatgtct ggaataacta
     3661 tggtatttat gccagcagtc actacttttg caatatcaag actgttaggt ggaggtaaaa
     3721 taatgctggt tggagatttg attgaacaac agtttactgt tgttggagat tggaactttg
     3781 gttcagctat atcgatattt atgatgatag taatattaat atctatgtct ataatgtcaa
     3841 aatttggaga tgaatctgac aaagaaggcg gtgggttact attttaaaat taaagaaaat
     3901 aacttctaat atatatttag cattagtatt cttatttttg tatgcaccca tatttgcttt
     3961 ggtactattt tctttcaatg attcaaaatc aatggcaaga tggaatggat ttacatggaa
     4021 gtggtatggt caattacttc aaaatgagag tataatgagt gctttatatt atactattgt
     4081 aatagctata ttagcgtctg taatatctac aatagtaggg actataagtg ctataggaat
     4141 acataagatg agaggtaaga gtaagaaatt aatactaaat gtaaactatc ttcctatttt
     4201 aaatacagag atagttacag cggtagctct tatgagtctg tttgtatttg taaaaatgga
     4261 atttggattt acaacaatgc tattggcaca tataatgttt tgtttgccat acgttatact
     4321 ttcagttttg ccaaaaatga agcaattacc tgataatata gaagatgcag ctatggactt
     4381 gggagcaaca cctatatacg ctcttagaaa agtaatttta ccacaaataa aaccaggtat
     4441 agtttctgga tttttaattg catttactat gtcaatagat gactttataa taagtttctt
     4501 taatgctgga aatggtgtaa gcaatctttc tatagaaatc tatggaatgg ctagaagagg
     4561 tataaaacct gaaataaatg cattatcaac aataatgttt gcagtggttc taggtttact
     4621 tttactagct aataaaaaag aatctatagt aagggggact aaataatgat taagtttaaa
     4681 aaagtaatat cccttatagc tgtaggtatg atgacagtta ctttatttac aggatgtaat
     4741 aaagcaacag attctacaaa agttttaaat gtatataatg ttggagatta tatagatgag
     4801 tctcttatag ataagtttga aaaagaaaca ggaatagatg ttcaatattc aacttatgat
     4861 actaatgaaa tgatgtatca aaaagtgaag agtggtagta cacactatga tttagtattt
     4921 ccttcagact atatggtaga gaaaatgaaa aatgaaggtc ttttggaaaa attagatttt
     4981 aagaatatac ctaatatgaa gtatttagat aagagttttt taaatcctgt atacgacaaa
     5041 actaatgaat atagtgttgc atatatgtgg ggaactcttg gaatattgta caataagaaa
     5101 gaagttaaag accctatgga tagctgggat atactttgga atccaaagta taagggaaat
     5161 ataatgatgt ttgactctgt tagagatact ataggaataa ctcttaaaaa attaggatat
     5221 agcatgaata gtgtaaatcc aaaggaaata aatgaagcta aaaatctttt aatgaaacaa
     5281 aaggatttag tactggctta tgtaaatgat gaaggtaagg atagattgct tggtggagaa
     5341 gtggcaatgg gtatgctgta ttctggagat gcagttacac taatggaaca aaatcctgac
     5401 cttgattatg ctatacctaa agagggaact aataaatggg tagatgctat gtgtgtacct
     5461 acaacagcgc aaaataagaa agaagctgag ttgtttataa actttttatt agacccagag
     5521 aatgcaaaag taaatgctga gtacattggg tattcaacac ctaatacagg agcactaaaa
     5581 ttattagacc cagaaataac tcagaatcct gttgcatatc catctaaaga agtacttgat
     5641 aaatgtgaaa cttttgaaga tattggtgaa aatataaaat tatatgacaa ggcttggata
     5701 gagttaaaat caaaatagag tcttgttggt ttaagtaaat ttacttaata aatacttaat
     5761 aaaactgttc ttttttagaa aatttatttt ctaattattg aatagtttta ttttttgttt
     5821 aagttatatt tttgtttagg tcatgttttt attttgagtt tatttttatg agaatttaaa
     5881 gtccagtaag tttcattgat tactattttt ataataataa ctataaagag gctgtctcaa
     5941 aattgagata agcttctttt taattgttga aatgatgaga acatgtttga tttcatagaa
     6001 aaaagagtga ttcatgagct aaaaagttca ctt
//
LOCUS       DAEMAE010000214         5999 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEMAE010000214 DAEMAE010000000
VERSION     DAEMAE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.216"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..528
                     /locus_tag="KPH93_003150"
     CDS             82..528
                     /locus_tag="KPH93_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689923.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSRK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKDEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            813..1517
                     /locus_tag="KPH93_003151"
     CDS             813..1517
                     /locus_tag="KPH93_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1689924.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKVNTVEESKKILKFGELSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            1504..2748
                     /locus_tag="KPH93_003152"
     CDS             1504..2748
                     /locus_tag="KPH93_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1689925.1"
                     /translation="MYLYNPEVKKFLSKYATLMFIVIIISIGFSVINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESDIVDIITQGKSTENIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILILVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAMQ
                     SMKIPMEIKNQNLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQSGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            2828..3505
                     /locus_tag="KPH93_003153"
     CDS             2828..3505
                     /locus_tag="KPH93_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689926.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDGEYFDDLMETLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            3506..>5999
                     /locus_tag="KPH93_003154"
     CDS             3506..>5999
                     /locus_tag="KPH93_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689927.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQIRETIKNDGSFHVTFYDVNRKNVEYVTKSAEVEKHAFTKQLGYSKLENSENGIVS
                     IKQYDKNAINGYKISIKEGRFPAKEGEIVLSDNIINLIDDNLKIGDKITLKVGDMFDS
                     SKKKIDSMVFHEGDYIANEKDRTFEVVGIIKKPGFERYNGIATAITYFNPMDNYNDTI
                     NVSVALRNPKDVYEISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYANINSSI
                     KSIIIAVTILVIICTIATVYNSFSISITERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGIISGTVAIDLLFKIINKHFTESVVTQMSLKIVYNPIIIIVSVIIVLFTIF
                     ISILLPAISASNISPLNVIKNNGEYKVGKVKSLRLIKMIFKTEGVLAYKNIRRNRKKF
                     TVTLFSLMVSIIIFISFSGFTLLLLKGEEIRNSQRNYDLYLTAKGTAKSIDDTISKLE
                     AISGIKNFELATGQYMSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKTNNLIQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKIKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFVA"
BASE COUNT         2506 a          543 c         1027 g         1923 t
ORIGIN      
        1 tttaaaaaat aacataattt ttattaaaat aatacagata ctatttatag atagaaataa
       61 aaattagaaa ggagaaaaaa tatgttaaca tatttaatga aaagacaaat gcataaaaaa
      121 gatgatggaa tgagtaaacc aatgatgatt acaggagctg taattacagg tgtaggagca
      181 tatgctgtat acaaaatggt aaaaagccgt aaatctaatt ctcaaatggt aaatacaaat
      241 tattatggaa atagcgatta tgatgattat aaatatgacg aatttgatta tgactgtgat
      301 tgtagagaca caaaacaaga ttatgaatat gaagaattaa aaaaaaaagt aaaagaattt
      361 aattcaagaa gaataaattg tgaaaattgt ccacatgttt ctcaagaaga aatgatgcag
      421 tatgtaaata gtgttaaaga tgtaaaagat gagattgatt tacatgaaga tgctaaaaaa
      481 gataatattt acaaagaaga tgaatacaaa aaatatgaag aagaataagt aatattctgt
      541 ataactgaac aaatagtaat gttaaattat ttaatatcta gttttaatat gtaagttgta
      601 acttaacttg aaagaatcag tatcattctt tagatagtta agttacaact tacattttgt
      661 ttttataaat tttaatagtt ctttgtgact attttgtaat tttagaaata ttttagtatc
      721 cagggtaatt gtgtataata aaacttgtat aggtagatag tgtaaaatta agcagatagt
      781 ttaaaattaa aatgaaaaga aaggaaatag ttatgttaaa gttgttgata gttgaagatg
      841 atagtactat ctcttttgga ataaagtatg ctttagaaca agaaggattt agtatagata
      901 tatcaaagga tttatcgagt ggaaaagaaa tgatttcttc taatgaatat agtatgatac
      961 tgttggatgt tacattgcca gatggaactg gttatgagct ttgtcaatat ataagaggat
     1021 tctctcaggt tcctataata ttcttgactg cttgtgatga agaagtaaat attgtaatgg
     1081 gtctcgatat agggggagat gattatataa caaaaccttt tagaattaga gaattaatat
     1141 ccagaataaa agccgttttg agaagaaagg ttaataccgt agaagaaagt aagaaaatac
     1201 ttaaatttgg agagttaagt atatatacat tagaagctag agtatataaa aacgataaag
     1261 aaatatttct gacatcagtt gaatataaac ttcttttaat tcttatacag aataaaaata
     1321 ctgttttaag tagaactcaa atattggaaa aactttggga tgtaacttat gattttgtaa
     1381 atgacaatac tctaacagtt tatataaaaa ggcttagaga aaaaataggt gatgatttat
     1441 gtgaaccaat ctacatagaa actgtaaggg ggattggcta taaatggata ggaagtgaaa
     1501 atagtgtatc tatataatcc agaagtgaag aagtttttaa gtaagtatgc tactttaatg
     1561 tttattgtta ttataatatc tattggattt agtgtaatca acgtgtcttt aactaaagag
     1621 atggttgtaa gaaataatca agctataata gggactttat cgagtaagta tccaaactta
     1681 gaaagtgata tagtagatat aataactcaa ggcaagtcta cggaaaacat agattatggt
     1741 cagaaaatat taagtaagta taattatgat aaatctatta agataaactc tgagcctatt
     1801 atttctaaat tagtcttaga gactatcaaa ataaatataa tattagtatg tataatattt
     1861 attttgatac ttgtgttggt tgttagatat tttaagtcaa tttacaatga tttatcagat
     1921 atgactaagt atgtttacta tagttcagag ggtaagccat ttgatatgaa aaacaagaat
     1981 caagaaggac agataggtct tctaaaaact gagctcttaa agatgacaac aattcttaat
     2041 gaaaaggttg aacttttaaa aagagaaaag atatttttaa acaatacaat ttccgatata
     2101 tcacatcaat taaaaactcc tatgacttct ttaataatgt tgaatgattt attatataat
     2161 gatgtaccat atgaagtaaa aatcgatttt ttaaataaga taaaaaatca attaaataga
     2221 atggattggc tgattaaaag catgcttaaa ttatcaaagg tagaagcaaa agtgataaat
     2281 tttaaaaagg ataaggtgaa atttagtgaa ctcatacata gagcaatgca atctatgaaa
     2341 atacctatgg agattaaaaa tcaaaatttg actatagagg gaaatgataa tgtatcttat
     2401 attggtgata ttgattggtc tgtggaagca ctagtgaata taatcaaaaa ttgtgtagaa
     2461 catactcctg aatttggaaa tataattata acctataaag aaaatccatt attttctgaa
     2521 ctaataataa aagatgaggg tgaaggtata gataaaaaag atataccaca tgtatttaaa
     2581 cgattttata gaggcagaag tagttcaaaa gaagatagtg taggaatagg tcttgcaatg
     2641 tcaaaatcta taatagaaag ccaaagtggc gatatatatg taaacagcga aaaaggtaag
     2701 ggtacagaat ttcatataat atttcataaa atgtatgata gtgactaatc tgtaatattt
     2761 aattcacttt atagtaatag ccaaagccta atatatgaag tatagaatat aaatattgga
     2821 ggtagttatg gaaattttaa gagtagaaaa tctaactaaa agttatggca aaaatgaaac
     2881 aaaagttgat gctataaaaa atgtaagttt atcagttgaa aaaggaacat ttatagcaat
     2941 aacaggtcca agcggaagtg gtaaaagtac cttattacat ttattaggag gagttgacag
     3001 gccaactagt ggtaaggttt atataaatga tgttgatata tataatttaa aaactaagga
     3061 tttggctata tttagaagac gaaatatagg tcttatatat caattttaca acttaattcc
     3121 agtattaaat gtgaaggaaa acatattgct tccagcagag cttgacaata gagatattga
     3181 tggagaatat tttgatgatt taatggagac attaggtcta attgatagag aaaagcacct
     3241 tccaaaccaa ttgtcaggag gtcaacaaca aagaacctct ataggacgtg cattaattaa
     3301 tagaccttct ataatattag cagatgagcc aacaggtaat ttagatagta aaaattctaa
     3361 agaaatacta gaattgttaa aattatcagt aaaaaaatac aaccagacac taataatgat
     3421 aactcatgac aaaaatatgg cacttcaagc ggatagaatt ataagtatag aagatggaat
     3481 cataaaaagt gatgaggtga tgtagatgaa tttatataca tcactaacta ttagatactt
     3541 aaaacaaaat aaaagaagaa ctattgtaac cataatcggt ataatacttt cgacagcttt
     3601 aatttgtggt ataggaaata tatttgaaag ttttatggat tatcaaatta gagaaactat
     3661 aaagaatgat ggaagtttcc atgttacatt ttatgatgta aatagaaaaa atgtggaata
     3721 tgtaacgaaa tcagctgaag tagaaaaaca tgcatttacc aaacagcttg gatattctaa
     3781 attagaaaat agtgaaaatg gcatagtaag tataaagcaa tatgataaaa atgcaataaa
     3841 cggatataaa atttctataa aagaaggtag atttccagct aaggaaggag aaattgttct
     3901 aagtgacaat attattaatc ttatagatga taacttaaaa ataggggata agataacttt
     3961 aaaagtagga gatatgtttg attcaagtaa gaagaagatt gatagtatgg tatttcatga
     4021 gggggattat atagcaaatg aaaaggatag aacttttgaa gttgttggta taattaaaaa
     4081 acctggattt gaacgttaca atggaattgc aactgctata acttatttta atcctatgga
     4141 taattacaat gatacaatta atgtatcagt agcactaaga aatcctaaag atgtgtatga
     4201 aataagtaat aaaataagta aaaatatata ttcaaataaa gataaagaag cagtttctga
     4261 tatgataaaa tacaatgaac atttattgag gctacaaggt gctagtaagt atgcgaatat
     4321 aaatagctct ataaagtcta taattatagc agtaactatt ttagtaatca tatgtacaat
     4381 tgctacagtg tacaattcat ttagtatttc tataactgaa cgaaaaaaac agtttgggat
     4441 attaaattct ataggtgcta catctagtca aattaaaaga ttagttttta tagaaggtat
     4501 tataattagt ttaattggta taccaatagg gataatatct ggaacagtag ccatagactt
     4561 attatttaaa attataaata agcattttac tgaatcggtt gttactcaaa tgagtttgaa
     4621 gatagtgtat aatccaataa taattattgt tagtgtaata atagttttgt ttacaatatt
     4681 tatatcaatt ctattacctg caatatcggc ttctaatatt tctccactta atgtaattaa
     4741 aaataatgga gagtataaag tgggaaaagt taaaagttta aggcttatta aaatgatttt
     4801 taaaactgaa ggagtacttg catataaaaa tataaggaga aatagaaaaa aattcacagt
     4861 aactcttttt tctttaatgg taagtataat aatatttata tcttttagcg gatttacatt
     4921 acttttatta aaaggtgaag aaataagaaa ttctcaaaga aactatgatt tgtatttaac
     4981 tgcaaagggt acagctaagt ctatagatga tactataagt aaattagaag ctatttctgg
     5041 aataaaaaac tttgaattag caacaggaca atatatgtct ataagagtaa gtgaaaataa
     5101 aataaataaa tctaaggaag atttaatcag aaaatattat caaaaatata agattggaga
     5161 tagttatgaa tatgatttca gtaataatga attaattttt ccaggagatt ttgcagtaaa
     5221 aaacaaaaca aataacttaa tacagggttc ttttaataag gagagagcaa ttgaagaaaa
     5281 tggtgttata ttagttagaa aaagcgcatt tgaagaaaat gggaagaaag gtgtagtaga
     5341 acttaccaac tataaagtag gagatacagt aaattgtgaa tatcttgatg aaaatgcttt
     5401 aagtaaaaaa ataaaggtca agattcttgc tataactgat gaagaacgtc taggtcttgg
     5461 ttatcaaaat atggggttac aatttataac ttatgatgaa gttgctaaaa atttaaattt
     5521 aaaattaagt aaaagcctta tatttattga ttcaggagga agcattaaga ctaagaagga
     5581 aatagaagct ttggcaaata aaaataattt taacttttat gatgaaagtg caagcaatga
     5641 aagtgaaaaa caagatttaa aggttattaa aatatttgta tatggtttta tagtagtgat
     5701 atctttagta agtgttacaa atattctaaa tacagtaagt acaagtataa atctaagaaa
     5761 aagagaactt gcagtaatac aatctatagg ggttactcct aaaggattta ggaaaatgat
     5821 atatttagaa agttttatat atggtatact gtcattgttg tttggaatac caattagtat
     5881 tggtataatt ctaattatga acaaacttat ttcaggtgta attgaatttt ctccgattat
     5941 accgtggaca gccatagtta tctgtatagt aagtgttttt ataataactt ttgtagcag
//
LOCUS       DAEMAE010000215         5989 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEMAE010000215 DAEMAE010000000
VERSION     DAEMAE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.217"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..478)
                     /locus_tag="KPH93_003155"
     CDS             complement(<1..478)
                     /locus_tag="KPH93_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689928.1"
                     /translation="MFPILLFDNNLNDFTIIVGAFFSLLIISLISGLLATLILPEKWV
                     FTVTRGGLFISLLITVLGGIWPMIGRFYPKEYKSTDIFKRSMAIEGLFEWLGLLCLIL
                     LIEIFARQSEFCEYIVSLGKSLLILHSIPFYPFECFGGKRIWNYSKILSIITIVISI"
     gene            complement(521..694)
                     /locus_tag="KPH93_003156"
     CDS             complement(521..694)
                     /locus_tag="KPH93_003156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689929.1"
                     /translation="MDNIIIREMVDDERRFVWELGKSSFGLVESLAFRKPKKAFIASG
                     FHLWSTSAQKSNS"
     gene            complement(931..3201)
                     /locus_tag="KPH93_003157"
     CDS             complement(931..3201)
                     /locus_tag="KPH93_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1689930.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIKDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIVRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSTLNNMKSNIKNFKSGSLSYNMSGNGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTMYILFIQKKSKEKLEDFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKRNLVLIYFDIDKFKLVNDRFGYEEGDRVLKGIAEIVKS
                     IFKEQSVFSRISNDNFAIIFEKKNDRESVVEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEDGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKIELSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENESFPTFPISINLSRVHLENPDFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGDAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSVKGK"
     gene            complement(3472..4926)
                     /locus_tag="KPH93_003158"
     CDS             complement(3472..4926)
                     /locus_tag="KPH93_003158"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG1689931.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLDDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGSIGQ
                     KYSDLCIITSDNPRTEEPELIIKDILEGIDKKKENYHVVVDRKQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            5090..5959
                     /locus_tag="KPH93_003159"
     CDS             5090..5959
                     /locus_tag="KPH93_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1689932.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDANSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         2085 a          959 c          624 g         2321 t
ORIGIN      
        1 ctatagaaat tactattgtt attattgaaa gtattttact ataattccaa atacgttttc
       61 caccaaaaca ttcaaatgga taaaaaggta tactatgtaa aattaataaa ctttttccaa
      121 gtgaaactat gtattcacaa aattctgatt gtcttgcaaa tatctctatc aacaaaataa
      181 gacacagtaa tcctaaccat tcaaataatc cttcaatagc catacttcgt ttaaatatat
      241 cagttgactt atattctttt ggataaaatc gacctatcat tggccatata ccacctaata
      301 ctgtaatcaa caatgatata aacaaccctc ctcttgtaac agtaaatacc catttttcag
      361 gtaaaattaa agttgcaagt agaccactaa ttagagaaat aatcaacaaa gaaaaaaatg
      421 cacctactat aatggtaaaa tcattcagat tattatcaaa taataaaata ggaaacaata
      481 tcaataagtt taaaatcaaa taaactccta attgtttaat tcatgagtta ctcttttgtg
      541 ctgatgtact ccacagatgg aatccagaag caataaaagc tttttttggc tttctaaatg
      601 ccaaactctc taccaatcca aaacttgatt taccaagttc ccacacaaac cgtctttcat
      661 catccaccat ttctctaatt attatattat ccaaattatc cctcccaagt tcttaaataa
      721 tttttaatat acttaattgc ataaactcta caattaaata aatgtttcta tcaaaacact
      781 tcatatactt taaacaaata tcatagtatt tctataacaa tttatattct taatagtagt
      841 atatttttat aaaaaaaaag tgagcactga ttaaacaagg ttcacttttt ttaattttta
      901 tattgtattt cataaattta tttaatttgt ttattttccc tttacactat atctatctaa
      961 tattaatttt tcatattctt ctattggcat tggtctagca aataaaaatc cttgagccat
     1021 atcacaccct ataaacctta aaaattctgc ttgctccaca gtctcaacac cttctgaaac
     1081 aacttttata tcaagttgct ttgccatttg aacaatactt gaaactacga tttgactctt
     1141 ttttgcatct cctactgtta taaaaaattg tctatccaat ttcaatacat caattggcat
     1201 atctttaagc atatttaaag aagaataacc agaaccaaaa tcatccatag aaattttaaa
     1261 tccaacagat tttaaagtct gcattatttt aaataatatt tttgtattat caaagattgc
     1321 actttctgtc aattcaattt caattaaatt tggctcaatt tcatattctt ttgttattct
     1381 ttcaagttca gatataaaat ctggattctc aagatgtacc cttgataagt ttattgatat
     1441 aggaaatgta gggaaacttt cattttccca acgtttaaaa tttttacaaa cttgagtaaa
     1501 tacaaacata tccaattctg ttataaaacc attcttctca aaaataggaa taaatacacc
     1561 ggggcttatt agtccctttt taggatgttg ccaacgtaca agggcttcag cacctacaat
     1621 tttagtagtt gacaactcga ttttaggttg taagtaaact ttaaattgtt cattttttaa
     1681 ggcatcatgc atttcttgtt caatatcacg ttcttctatg agagtttctt ttaaattttc
     1741 agcataaatc tcataaactt ttaaatattt tctttttact gtagtttttg caatcatagc
     1801 tttatcaaga caagtagaaa tatttgtttc tccatcttca acgaaatata cacctatgga
     1861 tggaattaaa ttgagttcta ctcccaaact tgtttttatc attgacaatt ttttacgtat
     1921 aagttcacat atctctacaa ctgactccct atcatttttc ttttcaaata ttatagcaaa
     1981 attgtcattt gaaattctag aaaatacaga ttgctcttta aatatacttt ttactatctc
     2041 tgctatccct tttaatactc tatctccttc ttcataccca aacctatcat ttactagctt
     2101 aaacttatct atatcgaaat aaattaaaac tagatttctt ttctcatgag aggataaaaa
     2161 ctttcctccc tcaagattaa atttatttga gttgccaatg cctgtaacgt tatccttaaa
     2221 agcaaagtct tctaattttt ccttactctt tttctgtata aataatatat acattgttaa
     2281 tgccgtaaat attataatca agactacaca tgctgataat gtaagtttta taatagcata
     2341 tgatttctca gatatagcag ttttaggaac ttcagaaaaa agataccaat cattgattcc
     2401 tatgggggca taacttaaat aatatccatt tccagacata ttataactta atgaacccga
     2461 tttaaagttt tttatatttg atttcatatt attaagagta gattttgata ttgaatcact
     2521 tctgccaatg taagaaaata aattttcttt agtagagttg gcgtttttac tgcttggatt
     2581 taaaacaata tcaccattac ttctaactat ataagaatat ccttttccat taaatgtagg
     2641 tacagaaagt attttttcat acaacttcgt actatgtgta gcaaatataa ctcctttaac
     2701 ctcattatct ttataaattg gtacagaata tacattaata ttactattgg tatttttatc
     2761 atcttttatt acatctttta attttcctgt tataaccact tctccttgca ttgattttaa
     2821 gaaataatct cttttagaga agtcttctat ataaccatct gtagtgtatg ctgtaccatt
     2881 tggtaaaata actcccattc ttttaaatga actattaata gcttgtcttt ttagtataga
     2941 aaggatatta tctacttcaa aagactcttc tccttcaata aatattgaaa ttgactctag
     3001 taactttata tctccattaa tttgtttttt taacacattg acgctttgat ttgctatttc
     3061 tgaaagatat tgattcgttt cttcacctaa tgtcttattt aaccttgaac cataaatcaa
     3121 acaggaaatt gtaattacta agatacttat gacagcaatt atggacaaac catacctatt
     3181 attttttgca tcacttaaca ttatgcttcc tgcctttctc gaatctatgt atgtataatc
     3241 attatcttat gacaattatt gtaaacattt tataataata cttatttttc catatttact
     3301 gtaattatac aaaattataa actttattac aacaataaat taattaatat agatattagg
     3361 gtttatatta ttgcattctc tattttacaa tcatttacat ataaaaaaat gtaatatcaa
     3421 agtgaaactt gatattacat ttttgttatt gtattttgtt tactttttat actacatttt
     3481 acttaaacac tcatttgcaa cttctttatc atcaaaatga tgcttaactt tacctataat
     3541 ttgataagtt tcatgtccct tacctgttat aattacaaca tcatcctttt ttgccatact
     3601 tatagcttct gctatagctt gttttctatc aacaacaaca tggtaatttt cttttttctt
     3661 gtcaattcct tcaagtatat cttttattat taactctggt tcttcagttc ttggattatc
     3721 agaggttata atacataaat cagaatattt ttgtccaatt gatcccatca aaggtctctt
     3781 ttcagtatct ctatctccac cacatccaaa tacagaaatt atttttcctt ctgcaaattg
     3841 ttgagttgtc tttagtacat tttcaagtgc gtcaggagta tgagcatagt ctacaattac
     3901 acttatacct ttatcatttg gaactgtttc aaatcttcct gatactccat tagaaagtct
     3961 aagaccttct ttatatattg gtttaggtat gccaagtaca tagcaagcag ctataactgc
     4021 taaagtatta tatactgtaa acattcctgg aactggtata aatattactt cttcatatga
     4081 aggtgttatt aatctataag atactccatc cgaatctgat tttatatctc tagccataaa
     4141 atcagcctta gtatcaacac cataagtgta acaaggtaca tttatacctt ttatattttc
     4201 atatatcttt tttccacctt catcatctat gtttattata tttgccatag tagtcttaaa
     4261 aaataatttt tcttttgctt ttctataatc atctaaatct ttatgaaaat ctaaatggtc
     4321 tggtgttaaa tttgtaaata tgcctagttt aaagtctgtt tcgtcaactc tatttaatgc
     4381 caatgaatgt gatgaaactt ccatcgcaca gtagtcacat ccattatcca acatattatt
     4441 aaaatgatat tgtaaatcta tactttctgg agttgttgaa ttggaaacaa tgtctttttc
     4501 tccgtcaaaa atttttatag ttcctataag tccaacttta ttcttattta aagttaatat
     4561 ctcatttagt atagtagtta tactagtttt tccattagta cctgtaactc ctataacgtt
     4621 aaatttttga gatggatgat tgtaaaaatt atcagctact tttgccatat cttttcttgt
     4681 atcttttacc tttataaatg tacatccttt tatttctaca tcttcttcaa caagaaatgc
     4741 acttgctccc ttttctatgg catcttttat atacttatga ccatctgaca caaatccttt
     4801 tatacatata aataatgtac cttttgtaac ctttcttgaa tcatattgta cattattaat
     4861 atctatattt aattcaccct tgacattgat tatatccaac ccctgtataa tatcatttaa
     4921 tttcataaat gctttcctcc ctttaaactc tttctgctaa aattacacta catataatat
     4981 tatattaaga attttttact atgtgaatgg ttaaaaatgc ttgaaacatt gcaaaaaatg
     5041 ctctataatt ttctataata taactgagga aaggtcttct ataaaaacta tggataataa
     5101 aatcggatac ttaaatgata actttaaaat atttaatatt agagataaaa aagatataca
     5161 atttgaatac cataatcatg attttaataa gataattata tttataaatg gaaatgtaac
     5221 ttattttgtt gatggtattc cttataaatt aaaaccctgg gatatacttt ttatcagcaa
     5281 caatgaaatc cataaaccag ttatagattc aaatgtattt tatgaaagaa ttgcaatatg
     5341 gattagtcca gaatttatga aaaaaaatag tgatgctaat agtgacttag aaaaatgttt
     5401 taaaatagct acagaaaata aatgtaatag attgcgttta aatatacatg atattgaatc
     5461 tattaagagt tttattgagc aaataaatat ttccgaatcc aatactgaat ttggaaatga
     5521 aatattgaga aacgcattat ttatacaact tatgatattc ttaaacagaa ttttcctaaa
     5581 aaatgaaaat atggaatcaa cagaagacgt cttttatgat gaaactatta agaaagtatt
     5641 aaactatata aattcaaata tagatgataa gctttcaata gatagtatat cgtctgaatt
     5701 ttttataagt aagtattatc ttatgagaaa atttaaatct catactggaa catctataca
     5761 caattatata gttcaaaaaa gattaatttt ggcaaagtca ctaataacca atggtcacct
     5821 tatgagtgaa gtttgtaata aatgtggatt caacgattat tctagttttg taagggcatt
     5881 taaaaaaaac tacggtgtat ctcctaaaaa ttatataaac aataaaaatc ctttagatat
     5941 tactctttta gatgaataga tactttatgt aaattttatt ttaataaaa
//
LOCUS       DAEMAE010000216         5897 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEMAE010000216 DAEMAE010000000
VERSION     DAEMAE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.218"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..1473
                     /locus_tag="KPH93_003160"
     CDS             109..1473
                     /locus_tag="KPH93_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG1689933.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKSVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LRDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYEDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLKMIPSGGCCINDTIT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKFL
                     KKIM"
     gene            1611..2777
                     /locus_tag="KPH93_003161"
     CDS             1611..2777
                     /locus_tag="KPH93_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1689934.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVLFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2894..3676
                     /locus_tag="KPH93_003162"
     CDS             2894..3676
                     /locus_tag="KPH93_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG1689935.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3795..4448)
                     /locus_tag="KPH93_003163"
     CDS             complement(3795..4448)
                     /locus_tag="KPH93_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689936.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4472..5836)
                     /locus_tag="KPH93_003164"
     CDS             complement(4472..5836)
                     /locus_tag="KPH93_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG1689937.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPDIEFNDLKNTLKYSQIASALTAIIGAVIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2223 a          754 c          897 g         2023 t
ORIGIN      
        1 tgttatattg agatgaattt attatgatat aaaaaataat gttaggataa atttaaatct
       61 ttttctaaaa atgtattagt ataattctta aagaaaggat ttttaacaat ggatataaaa
      121 gagttagtta aaatgcaaag aaaatattat aatacaggta aaacaagaga tatatcgttt
      181 cgtatagaac agttgaagaa acttaagtca gttgtatcac aaaatgaaga aaaaatactt
      241 ttagcactaa agaaagattt aaataaatcg gattttgaag gttttatgac agaggtaggc
      301 atgttttata gtgaattaaa ctttgctata aagaatataa gaaaatggag caaaataaaa
      361 agagttaaat caagtatggt taattttcca tctatatcca aagttgttcc acaaccttat
      421 ggtgttactc tcataatgtc accttggaat tatccatttc aattagcact tataccatta
      481 gtttggtcat tagcagcagg gaattgtgtt atattaaaac cttctgaata ttctgtgtca
      541 acatctagtg tagttaaaga catagttgaa aatacttttt caaaagaata tgttgcagtt
      601 gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg aaagatttga ttatatattt
      661 tttacaggaa gtactaatgt tggtaaaata atcatgaaat cagcatcaga gcatttaact
      721 cctattactt tagaacttgg aggaaaaagt ccttgtatca ttttaagaga tgctaatata
      781 gaccttactg ccaaaagact tacttgggga aaactaataa atgcaggaca aacctgcgtg
      841 gctccagatt atgttttagt gcatgaagat agaaaaaatg agttaataga gaaaataaag
      901 tattatacta ataagtattt tggagataat ccatgtaata atgagcaatt tcctaaaatc
      961 ataaatcaga gacattttaa tcggatatta tctcttattg ataaagataa gatagtctat
     1021 ggtggaaatt ataataagga gacactaaag atagagccaa ctatagtaga taatgttaat
     1081 tgggatgata atataatgaa ggaagaaata tttgggccta tatttcccat tttaacatat
     1141 gaagatttgg atgaagtaat acaaaaaatt atacaaatgc caaatccatt agcactttat
     1201 atttttacaa aaaataagta cctagaaaat aaacttttaa aaatgattcc atcaggtgga
     1261 tgttgtataa atgatacaat tactcatata gctacgaatt acttgccttt tggtggtatt
     1321 ggtgaaagtg gtatgggttc atatcatgga aaggctggat tcgatacatt tacgcactat
     1381 aagagcgtat taaaaaaatt aaatttggat gtccctatta gatatgctcc atatgataat
     1441 aaattaatta aatttcttaa aaagataatg tagttgtatt atggaaaaat aaatataaat
     1501 aaaattgagc atttgtattc tgaatatatt ttatttttaa tttggtatat ttgttataaa
     1561 agtggtgatt ttgtgataaa atttaattat atagaaataa ggaggaaaaa atgaagatta
     1621 attggaatac aaaatacaca acaatagcaa tttatacatt tataatagct gctagtagta
     1681 ttatatttta tctagtatca tcacaaattg atgtattttc taataattta gatgctatat
     1741 ttacaacatt acaacctttt ataataggat ttgctattgc atatcttctt aattttattt
     1801 taaagtttta tgaagacaga atttttataa aaagtgagaa attaaaaaaa ctaaaacaaa
     1861 gttcaaaaag aggcttgggt cttttattaa catatgcaac agctacattg atactttact
     1921 tatttatgca tttcgtattg ccacaagtga ttgagagtat tgttggacta gcaaatgata
     1981 tacctatgta tgtaaacaat gcaactaagc ttattgataa attaatgact gatttaaatc
     2041 ttgatgagca atactttaat ttggcagttg ataagtggaa tgagtttgtt acatatatta
     2101 taaagtttgt aacagattta atcccaatat taggaagtat gttaaagaat gtagcctcta
     2161 gtatatggaa tattgtacta ggattaattg tttcagtata cttattaatt gataaagaaa
     2221 aattctatgg attaagcaaa aaaataactt atgcagtatt tacagaaaaa caagctgcca
     2281 gaattttaga attaactcat agaagtaact atacatttgg aagattttta ggtggcaaaa
     2341 tattagattc attcattata ggtattctaa catttgtaat acttacatta gttaaaatgc
     2401 catatacatt attaatatca gttataattg gtattacaaa tattatccct ttctttggac
     2461 ctttatttgg agctatacca tctacactaa tagtgctgtt tgtatctcct ataaaagctt
     2521 tctggttatt gttaattata ttaataatac aacagataga tggaaatatt ataggtccta
     2581 aaatacttgg agattcaatt ggtatatctg cattttggat tttgttctcc ttattggttg
     2641 caggaaaatt attaggtttt attggaatgg ttataggagt tccaatgttt gcagtaattt
     2701 attctataat taaggatatt gttgaaagta aattggacaa aaagggatta ccaacagata
     2761 cttccgatta tatgtagcta aaaaatatta tgtataatta aaaatatata aattaagcaa
     2821 tatttaaagc actgagaaag ttaaactctt ggtgcttttt agatataaaa ttattataga
     2881 agaggtgttt tttgtggcat ttatagaatg taaatttaaa tctaaatata ctggaggaga
     2941 aactgatata attgtaattt tgcctgtagc aaatggaaaa gaacttttta gtggtaaaga
     3001 gatatatgat tttgaaggta aatttaaaac attatactta tttcatggtt tagaagatga
     3061 ccaaagctca tggattagat acactaatgt agaacattat gctaaaaata aaaatatagc
     3121 cctagtaata ccaagagtag aaactagttt ttatacagat atggcgtatg gacaccaata
     3181 ttttactttt gtaagcgagg aattacctaa atttgttaga gctatatttc ctttatcaga
     3241 taaaagggag gataactttg tagctggtat gtctatgggg ggatatggag ctttaaaatt
     3301 agcactttca aagccaaaag agttttcggc agttgcaagt ttttctggtt cgcctgatat
     3361 gattcatgag ttagaaaatc aacaaataga aaccaatgca gacattctat ttcacgattt
     3421 tggtagacca gaagacgcta gaaatagtga gaatgattta ctttacatac ttaagaacct
     3481 taaagaaaga aatgaagata ttcctaaaat ttatcaattt tgtggtgata gagatttttt
     3541 atataaaaat aatcagatgt ttaaaaaata tgcagaatct ctaggtgtag atgttgaata
     3601 tgaagagtgt gtaggtgggc atgaatggcg tctttggaat ttatggataa agaaatttat
     3661 agatataatt gggtagtaca cttatggtat taacagagat attataaaat atctttaaat
     3721 atgataaaga ctgaaatcct aaaatcaaat tatgatttta aaatttcagt ctctaagaat
     3781 ttaataaaat tttattaagt tcttacacga gttattattg gagtaaaatt gtcaactata
     3841 tctatatagt atttactcaa ttcctctaaa atctcttcgc ataaatccgt actatgacca
     3901 agattaataa gaaactgttt tgtaaaatgc tttattacag cttgagaaga tatatcgtac
     3961 ttgtgtgaag tataaactct aattatttca ggtttcatag caagtatacc aatacaagaa
     4021 agtattaact catcatgttc tatagattct ttatcattaa catatctttt catgaccatt
     4081 tcggttacac ttctttgata gcttaaaata gcttcttcat aagctatatc caaataaaaa
     4141 tttctatatg cttcgttttt tagaaggaaa caaaggtcag cattcatagc aagaaagaac
     4201 atcatcatag taccttcttc gtgatagtta taaattaaag actgcatttc atttcttata
     4261 tcttcatata ttatagctcc tatttcgctt ttacttgaga agtaatagct tattagacct
     4321 aaatttatat tagctctttc tgcaatttgc ctagttgttg tcttatgata accttgctca
     4381 aaaaataatt ctctagcagc acttataata ctagcttttg tttctttgcc cttttctgat
     4441 tttgacatat ttactgtgta tacctctctt ttcaatcttt ttttgaaaat ttgctaacta
     4501 taaatattgt aatagaacca cctattatag gaaccaaaat tctatcaaat acagtaagaa
     4561 taccaatcat atttaataat gccaatataa cacttactat aacagcatag atagctcctt
     4621 tttttgtagc acctttccag caaagaccaa ttacaaatgg tgcaaatatt acacctgtac
     4681 atagtggagc cggccaaaac aataattcta ataaactatc aaatttaagt gctatgactg
     4741 cacctattat agctgtcaaa gctgatgcaa tttgagaata ctttagagtg ttttttaaat
     4801 cattaaattc tatatcaggg tgtaacaatt tgttataaat atctcttgat ataatagcac
     4861 tcattgaggt cattaatgaa tcggcagtag acattatggc agccaatata gcagctaaga
     4921 ataaaacaga gattaaaggt ggcaaagtag tcgatatcat ttgaggtaaa acctgaacag
     4981 atgatatgtt tggaaataaa ctaccaccaa tagctccaat tattcctata gggaaagcaa
     5041 gaaataatag tataaatcct gcaattacac tacttttaac tgcattttta gcattatcag
     5101 cacattgata tcttaaaaag ctttcataag aaaatattga gcctaaaact aatggtaaaa
     5161 ctgtatacat aaaatcacta atcgatttat ttcctggaaa gaatggttct tttaccattg
     5221 attgtaacat aagtaaaggg ttgtcgccaa ctaattttaa gcttatataa gctactattg
     5281 gcaaaaacac tatacaaata gcaagttgaa taaatgctga tactgcaact ccccacattc
     5341 ctgatgtata agtatatata atgataacta taactcctat aattacagct atatcataag
     5401 gaataccaaa tgcagtagat acagaaccaa aagaaattaa ttgactaccc ataatagcta
     5461 gtatagatat tggtgttaca agactttgat aagctcttaa aaatacacta tgaccatatc
     5521 tagtttcaat ataatcagca ggtgttatat cacaagataa gcttcttaat tttcctgcga
     5581 aaaatgcatt tataaagcaa gctaaagctg cacctatagt agaatacatt ccaacccaaa
     5641 gtcctctttc agctccccat tgaactccac caactaagca ccctcctcca aaagatgtag
     5701 cagcaatggt tgcagctaat ataggcaaag gaagatttct acctgcaatt aggaaatcgg
     5761 atgctgtttt aacttttttt gaaaaataaa aaccaatcca tatgtatatt gcacacgtaa
     5821 caattaaaag taacatagtc tcttctcctt tataaaatat tttaataaaa tatataaaat
     5881 atattttata taaaagt
//
LOCUS       DAEMAE010000217         5836 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEMAE010000217 DAEMAE010000000
VERSION     DAEMAE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.219"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            50..835
                     /locus_tag="KPH93_003165"
     CDS             50..835
                     /locus_tag="KPH93_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG1689938.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNSTNLDKKQSIAKYASKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1114..3024
                     /gene="phnW"
                     /locus_tag="KPH93_003166"
     CDS             1114..3024
                     /gene="phnW"
                     /locus_tag="KPH93_003166"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG1689939.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLDIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKKYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3379..4200
                     /locus_tag="KPH93_003167"
     CDS             3379..4200
                     /locus_tag="KPH93_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689940.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINIEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGISNFNEVGASI
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKVKLKKDK"
     gene            4241..5008
                     /locus_tag="KPH93_003168"
     CDS             4241..5008
                     /locus_tag="KPH93_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG1689941.1"
                     /translation="MEKGSIKILGEGFISSGEYESIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNLVVNGKTIIIGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            5016..5636
                     /locus_tag="KPH93_003169"
     CDS             5016..5636
                     /locus_tag="KPH93_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG1689942.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSTITFEELKLIVNIIEENFMSIYNENGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEVNDISIKLS"
BASE COUNT         2455 a          501 c         1092 g         1788 t
ORIGIN      
        1 cattgtaata ttataggaga aaaatatatt atatttagga ggaaaacgga tgtctttaaa
       61 ttcgatagat agaaaaaaag aaatacttaa attattagat tcaaaaggga aggtaaatac
      121 taaagagtta gtaaaaagat tagaagtatc atcagaaaca ataagaagat atttggaaga
      181 attagaaaat gaaaataaat taaaaaaggt atatggtggt gctataaaaa taaattatgg
      241 agtgattgaa ccagaacata taaagagaaa cagtactaac ctagataaaa agcaaagtat
      301 tgctaaatat gcatctaagt ttgtaaatga caatgagtca atcatcatag atgaaggtac
      361 taccaatttg cagattgtag attatataat atctgtaaac aatttaaaaa ttattacaaa
      421 ctcttatcca gttctatcaa aattgatttc ttacgagaat caaggcttat ttgatggaga
      481 agttatattt attggtggaa aagtaaattt aaaacaccaa aggacagcat gtccaatatc
      541 aatagacact atgaaaaact tatacgtaga taaagctttt atatctagtg aaggtatact
      601 tgatagtttt ggaattagta gtatagacca aaataaggca ttgttgtcaa aagaatatat
      661 aaaaaattca aaggaaagtt ttgtattatg tgatagttct aaaataggaa ttgctagtat
      721 gtataagata gcagatttag aggaattaga ttttgttatt tgtgatgtag aacctccgaa
      781 tgaatggaag gataagttaa atgagataaa tataacatgg atagaagcaa aataaaccct
      841 tagttttata taatacaact atgcttaata taactaaatt cataaaatat agttagttca
      901 tagattaaga ttagaaagta aattttagat tgtaacttag gattttatgg aaatacgata
      961 caaaataaaa acaactaaat acaactaaaa acagaaattt aatatatatt taacctaaat
     1021 atgatgtata attaacatat gaaagttata ctttaaaagt aaaaacaatt aaaaacacaa
     1081 aaattctaaa aagaatctat ggaggctata aatatgaaaa agatatatgg agaaaaaata
     1141 aaagcagtag tattcgattg ggctggaaca acagtagatt atggatgttt tgcacctctt
     1201 aatgtattta tagagatatt taaaagaaga ggtatagatg taacaatgga agaagcaaga
     1261 aaacctatgg gaaaattaaa aatagaccat ataagagaaa tgtgtgaaat ggatagaata
     1321 aaaaatcttt ggtcagataa gtttggtaaa gtgccaactg aagacgatgt aaatgaactt
     1381 tatgctgaat ttgagccaat gttatttgag acattagaag attatacgac tccaattcct
     1441 catgttgttg aaactattga aaaattgaga aaaaatggat taaaaatagg ttctacaacg
     1501 ggttatacaa gagagatgat gaacatagtt gaaccaaatg cagcaaagaa aggatactca
     1561 ccagattttc ttgtaacacc atcagaagta tctcaaggaa gaccatatcc ttggatgtgc
     1621 tataaaaatg ctgaagcttt aggggtgtct cctatgagtt ctatggtaaa agtaggagat
     1681 acaataagtg atgtaaaaga aggcgtaaat gcaggaatgt ggagtgttgc tgtaataaaa
     1741 ggttcaagtg agttagggct tacacaagaa gaagtagaga atatggataa ggaagaatta
     1801 aaagctaaga tgagtatagt ttcaaagaaa tttaaagagg caggtgcaca ttttgtaata
     1861 gaaactatgg ctgagttaga agatatacta ataaaaatag agaacgaaac tattaaatca
     1921 gattttgtac cagaaaatga ttacatatta cttacaccag gaccattatc tactactaaa
     1981 tcagtaagag catctatgtt aaaagattgg tgtacttggg atgttgaata taataaccta
     2041 gtacaagatg ttagaagaag attagtatct ctagcaaccc aaaatacaga taaatacaca
     2101 agtgtattaa tgcaaggaag tgggactttt tcagtagaag ctataatagg aagtactatt
     2161 tcaaaagatg gtaaacttct tgtaattgca aatggagcat atggaaagag aatgaaagat
     2221 atatgtaatt atcttgacat agaatttgtt gactgcacat ttaaagatat agaagcagtg
     2281 gatttaaatg tagtagaaaa tttattaaaa gaaaacaaag atataacaca tatatcaatg
     2341 gttcactgtg aaactactac aggtagatta aaccctatac aagaggtagg gaaacttgca
     2401 aaaaaatata acaaaattta tatagttgat gctatgagta gttttggggg aattgagatt
     2461 gatgttgaag attttaatat agatttttta gttagtagtt ctaacaaatg tatacaagga
     2521 gtacctggat ttggttttat aatagctaat aaagaggaat tatctaaatg taaaggtata
     2581 gctaaatcat tatctcttga tgtgtatgcg caatgggaga ctatggaaaa aaataatggc
     2641 aaatggagat ttacttcacc tacacatgtt gtaagagctt tttatcaagc cttattagaa
     2701 ttagaagaag aaggttctgt tgaaaaaaga tatgctagat ataaagaaaa tcagtttaca
     2761 atagcttcaa gattaaaatc tttaggcttt gatactttgg tgaatgataa tgctcaatca
     2821 cctgtaataa caactttctt atatcctaaa aatgcaaagt ttgaatttat ggaattttat
     2881 acttatttaa aagataatgg atttgttatc tatccaggta aattgactga tatagataca
     2941 tttagaatag gtagcatagg tgaagtttat ccaactgata tggagagact agctgatgta
     3001 atagaaaaat ttattaatag atagaagaag atagtgtctt gtagcttaca gaatagtgca
     3061 gaaagcccac tcctttaggt gtgagatagt acaaacagtg tataagaagt attgacaata
     3121 ggatttaaag acttatattt tagaagttta tacaatgaat atataaagat tgagtaatag
     3181 ggatgtattt tacttaaacc acaatctaag aaattagatt tatagtttaa ataaagcatt
     3241 ttccctatta tttttatgcc tatttataga aaataaataa tagttgtttt attaagtatt
     3301 attatcatac ttgacaacgt aacaatgttt tgttatatta tgtttaaaga tagaatacgg
     3361 taaagaaagg atatcattat gaatgaagaa ttaagttcac taagatttga tggtgttgga
     3421 aaatgtaaag gtggaatttt tgataatgtt aatatatcag gaacaggaaa aatagaagga
     3481 agtgtaaaat gtagtaaatt tgattcttct ggttcagcaa aagtttttgg gacaacagaa
     3541 tgtaatgaat tttttacatc tggtttaagt aaagtagagg gaaacatact ggcatcaaaa
     3601 gttgagatat cggggcttct tagatgtaca ggaaatatta attctgttga aattgatact
     3661 agtggaatta tcaatataga aggaagtata aaatcggata ttattcatgg agaaggatat
     3721 ttaaaagcaa atgagaatat agattgtgaa aatatcaatt taagtggaat cttggagtgt
     3781 aatggatttt tgaattgtga agaagttaat attagtttaa atggaatttc taattttaat
     3841 gaagttggag caagtattat aaatataaaa aaggggcatg gagattattg caaaaaattt
     3901 cgtatgctag aaaaaaagca tatcaacttt gaaaaagtgt cttcaaattt aataagtttg
     3961 ggtctgttat caattagaaa gtatacagaa agtaaattgt ttgcaaatat tatagaagca
     4021 gatgaaataa gcttagaaaa tagtgaagtt aaagttgtaa gaggaaaaaa tattaaaata
     4081 ggcaagggat gttatgtaga aagtttggag tacagtgaaa gtatagaaat tgatgaagat
     4141 tcaatagttg gtgaaataaa gaatataagt gaaaaagtaa aattaaagaa ggataagtaa
     4201 taagtaggtt taaaaataaa atttaaaatg aggtaataat atggaaaaag ggtctataaa
     4261 aatacttgga gaaggcttta taagtagtgg tgagtatgaa agtataaaaa tcgtgggaag
     4321 ttctaaatca aaaggaaatg ttaaaagtaa cgaagtaaaa gtccttggag atgcaaaatt
     4381 tgaaggtgat atcaatataa aaaatttggt tgttaacgga aaaactatta taataggcaa
     4441 tcttaaagca gagcgtgtaa gagtgaatgg tcaacttaac atagagggag atgcagaaat
     4501 agatgactta gttataactg ctcaacagaa agtggatggc aaacttaaat gtaataatgt
     4561 tacaataaga ggacaattaa gtgtaaatga aaatatgtat gcacaagata taaaaatcta
     4621 tggcgaaatg agaaataaat caaatataga atgtgaagat ataaaggtat atggagtgat
     4681 aaaatgtgat ggtcttttaa atggagaaaa tatatatgtt tattcaggag gaggttctta
     4741 ttgtaaggag ataggggcaa ctaatataaa aataggaaga agcaaagatg ctttttcatt
     4801 aattaaaatt attaatattt tttcaggaaa gtttaaatgt aatttgatag agggagatac
     4861 tattgaatta gaagatgcat caataaaaga tatacgaggt aaaagcatat cgctagtaga
     4921 taattgtgat gttggaaata ttgaatacag tgataattta tcggtttcag ataattctac
     4981 tgtaaaaaac agtataaagg tgggctagaa gagttatgga agagttaata tctaagaaag
     5041 atttattgag caaaactaat atatcttatg ggcaattata tagatggaaa agaaaaaaca
     5101 taataccaga ggaatggttt ataaaaaaat cagcttttac aggtcaagag actttctttc
     5161 caagagacaa aatactagaa agaatagaat taatattgtc tatgaaagaa gagacttccc
     5221 ttgatgatat tgcaaatatg tttatgaaaa aagatattaa taaagaattt gacattgatt
     5281 ttattctcag taaagaagtt atatgtaatt acactaaaga aatatttcaa aatttatata
     5341 agaaagagca aagcataggt aaaaaagaac tgcttatatt gagtataata gagaaatttt
     5401 tagttaaatc tacaataact tttgaagagt taaaattgat agtgaatata atagaagaaa
     5461 attttatgtc aatatacaat gaaaatggaa agatttattt gtttagaaag tttggagttc
     5521 cattcgtagt aggatgtctg gattataaaa aagtaaaatt tgaaaaagat gtagttaaga
     5581 ttattgagat tgacttgaca aaagaagtaa atgatataag tataaagtta tcatagtgca
     5641 ttatcttata taatgtatta taataatcta cttaaaatac actttattta aatatttagt
     5701 ttaaagatga gattttatgt gagtatgtaa aagttaaaga tagatttaaa tagattatag
     5761 ggattagtaa acaataaaat gaaccatgat gaactactat aaattgtatt taaactacat
     5821 tttctactta tagtaa
//
LOCUS       DAEMAE010000218         5682 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEMAE010000218 DAEMAE010000000
VERSION     DAEMAE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.220"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(75..761)
                     /locus_tag="KPH93_003170"
     CDS             complement(75..761)
                     /locus_tag="KPH93_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG1689943.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKKRLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQGAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLKEKEIKKVALLGTKYTMEQDFYKNIIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIVSESSKKAFLSIIDKLSKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            886..2268
                     /locus_tag="KPH93_003171"
     CDS             886..2268
                     /locus_tag="KPH93_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1689944.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTVYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVVDKVLSDYNLIGHKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            2670..3938
                     /gene="eam"
                     /locus_tag="KPH93_003172"
     CDS             2670..3938
                     /gene="eam"
                     /locus_tag="KPH93_003172"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG1689945.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(4031..4387)
                     /locus_tag="KPH93_003173"
     CDS             complement(4031..4387)
                     /locus_tag="KPH93_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689946.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(4538..5632)
                     /locus_tag="KPH93_003174"
     CDS             complement(4538..5632)
                     /locus_tag="KPH93_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1689947.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         2232 a          765 c          807 g         1878 t
ORIGIN      
        1 ctccagtaat ataaattata aaaagtctta tttacacaaa tctatagata ttctttttgt
       61 taattaatat tgtcttaaat tgaacttagt gcggcttcta ttgcatgtat tactgttgta
      121 tcaaatagtg gaatagaagt gtcattttgt ttaattaaaa gacctatttc tgtacatcct
      181 aatataacac cctctgcacc ttgtttgcta agcttatcta taatagataa gaaagctttt
      241 tttgaagatt cagatacaat tcctaagcaa agttcgttaa atattgtatc atttactatt
      301 atacggtctt cttcatttgg aattaagact tcaattccat tattaataat tatgttttta
      361 taaaaatctt gttccattgt atatttagta cctaataagg ccactttttt tatttctttt
      421 tcctttaata ccgtagcagt tacatctgct atgtgaagta atggaatatg tatactttct
      481 tgtattttat ctgatacctt atgcatagta ttagtacaga taacaataaa atctgcccct
      541 gccccttgta atttaattgc agcgtcagct aaaatcttgg cgcttttttc ccaatttcca
      601 ctagattggc attcttcaat ttcttgaaaa tctacactgt aaagtataca cttagaagag
      661 tgtagtccac ctaatctttt ttttattact gtattaataa cttgataata tgtaatggta
      721 ctttcccaac tcataccacc aattaagcct atagtcttca tattatacct ctcttattta
      781 tttattttga aatagctatt tacaaataca agtataacat ctaaaataga agcatacaat
      841 aataaaattg tatggagtac aattataatt aagaggagta attatatgcc aataaattct
      901 tttgaaaact atcctatgac atggaaacca aagttagata accgaaaacc accaatatat
      961 aaaactttag caatgttatt agaagaagat attaaaagag gcaatttaaa tcctggagat
     1021 aaactgcctc cacaacggga attagcagat tttctagatt taaacttaag tacaataaca
     1081 cgagcattta aactttgcga agaaaaggga ctaatatgtg caaaagtggg aaagggaaca
     1141 ttcatttctt ctgatgtaaa tgtaagtaat acattattat atcaaacgga atcaaaagac
     1201 ataattgagt tagggacagt tcatcctccg tatgagcaaa atacatatat tattgacttt
     1261 attaaaaatg tactaaaaca accagaaatg gataggtttt tacaatacat gtctccatct
     1321 ggcacatata tgcaaaaaaa gtcaattgca aaatggatag aaagaaataa tgtatacacc
     1381 agtgaagaaa atatattatt atctacagga ggtcaaaatg ctatatgtgc aactttacta
     1441 ggattattta aagctggtga tagaattgct acagattctt taagtttttc tggaattaag
     1501 tcaatagcta aaatgatagg tattcaatta gttccaattc cacaagaaaa taatgaaata
     1561 tcaattgaat atcttgaaag ttattgtaaa aatgaaaata taaaaggaat atatcttata
     1621 ccagattatc ataatccaac gacacatact atgtctgatt tatctcgtaa aaagatagcc
     1681 aaaatagcaa agcaatataa tataataatc attgaagatg ctataaatag catttttaga
     1741 gacggtatac aaactcccat attttcttta gctagtgata atactgtata tatttttagt
     1801 acatctaaat ttttatgtgc tggtcttaga gtagcatttg tagttgctcc aaaacgatat
     1861 atagaaaatt tagaaaatgc actatataat atgaatctaa tggtttctcc atttacagca
     1921 gaaatagtac accgtttatt atattctcct attatagata aaattataga agagaaaaga
     1981 gatgcaatta tagaaagaaa tgaagttgtt gataaagttt tgtcagatta taatttgatt
     2041 gggcataaga attgcagttt tcgttggtta cttcttcctg atgaattgga tggaaaatca
     2101 tttgaaattt ctgcaaaaaa tctaggagtt caagtatatt gtgctgaacg attttcagtg
     2161 ggaaactcta cagttccaaa agccgttcgt atttgtgtaa cagctccaaa agatgtagaa
     2221 gaattggaaa aagggctcaa tataataaag tcacttctta agagttaaat ttattttact
     2281 attatttaat taaataaata aaaaggcgga gaaatttatt ataataaacc tccgctttta
     2341 ttatttaatg aaatacttta ctttttatta aaaaatttaa ttttaataat cttgaaaata
     2401 taatcgtttt atggtaaaat aattcttaaa aatagaaaaa attaatcatt tgttaaaaaa
     2461 aatgcataat ttattgtaat ttattgacga aggttgaaaa atattcataa atgtgcgaaa
     2521 taacagaaaa aatgcaaaaa ttaattcaat ttcatttgtt ataaaatcta tatggcaaaa
     2581 ttaaatctgc ctatcagaat gatataaaca catcagaaag gaggacgcta tcaaataata
     2641 caagtagtta actttgatag caaaaaatta tgaatgaaca aactagaata tccttagaga
     2701 gagctgctga attaaaatca aaaattgatg attatattca ggctagaaaa acgattaaca
     2761 gaggtcttga aaaagaagaa gagataaata aacgaaaaca gaaaatatta agtatcttaa
     2821 atggaactga agaggattgg aataactaca aatggcaatt atcaaataga ataacagatg
     2881 tagatacttt atcaaagatt ataactctaa ctaaaaaaga aaaagaatat ataaaagagg
     2941 ttggtactca atttagatgg gcaatatctc catattattt gagtcttata gacccagaag
     3001 atatatgtga cccaataaaa ttactatcta taccaacaca tatagagttg gaagatgaac
     3061 aagaagattt ggacccaatg ggagaagaat atacaaaccc agcaggatgt ataactagaa
     3121 gatacccaga tagattaata ataaatgtaa caaatgagtg tgctatgtat tgtagacact
     3181 gtcagagaag aagaaatatt ggacaacaag attctcataa gtcaaaagct attatccaag
     3241 aatctataga ctatatcaga gaaaatgaag aaataagaga tgtactagta actggaggag
     3301 atgctcttac attaaaagat gattatttag agtggattct tagtcaactt aaagagatac
     3361 cacatgttga ttatgttaga ttaggtacta ggactcttgt tacaatgcca caaagaatta
     3421 cagatgaatt ttgcaacatg ctaaaaaaat atcatcctgt atatataaat actcatttta
     3481 atcatccaat ggaaataact aaggaatcta aagaagcttg tgaaaagtta gcaaatgcag
     3541 gagttccatt agggaatcag gcagtattat taaatggaat aaataatgat aaatttgtaa
     3601 tgagatgttt aaatcaagaa ttactgaaaa taagagtaaa accttattat atattccaaa
     3661 gtaaacatgt aaagggaaca aaacatttca atacatcagt agatgatggt cttgaaatca
     3721 tggagtattt aagaggatat acatcaggaa tggctatacc aacatatata gtaaatgctc
     3781 caaaaggagg aggaaagact cctttgcttc cgcaatacct tgtatcaaaa ggaacagatt
     3841 atgttatgct tagaacatgg gaaggaaaag ttataaaaat ggaagatgaa cctgctgtag
     3901 atataaagaa acttataaaa gagcaagcac aggactaaaa aagtatttaa agtctataaa
     3961 aagaaacaac tttaaaataa aaagaaaatg gatgccgaat ttatatcaat ttgcatccat
     4021 tttttattag ttatttatta ttttgttgtt gattaactaa ttcaagatat ccacttgggt
     4081 ggaagtaata tatctcagaa gtattttcat ttacaaagta accaatatca ccatctgcat
     4141 ctggtaaaaa tacataacca ttaagatttt tagcttttaa tgcttcaaaa tcactagcat
     4201 ttccttgata tatgtaatca acttcattgt tgtattttac taaaagttct ttagcttgct
     4261 cttgagtcaa tttagtattt tggcttacag gaacattctt ttgagaatct gcataagaag
     4321 attcatttac tggtgcagtt aataacaaac aagataaaac taataaagat ataagtgctt
     4381 ttttcatact aaaaccctct ttcatgttta attatataag attcattact taataatatt
     4441 gtatgtttcc tataattaaa tattaccatt aaagagaata atattacagt tacacagctg
     4501 taacttttga ttacaaaatc gtaattaaat tgtattttta ttccatttgt atcttatcat
     4561 gtctacgatt tacaaattca cagaaatata tctttataac acccatcaca ggagcagata
     4621 gaatcatacc aggtatacca aagaatcctc caccaacagt aactgctagg attgtaaaga
     4681 atgggctaag acctagttgt ccacctacga tttttggttc tataaatgta acttcaactt
     4741 gttgaattat tattaagtaa attaaagaag ttaaagcaat tgttggattg gagaataaat
     4801 taattattac aactggtgcc atacctatta ctggaccaaa atatggaacc atattcatga
     4861 cacccataag tgtaccaaat aatagtgcat attcagattt taaaaagtaa agaccaattc
     4921 cagagagtac tccaactata aaagaatcta aaatcttacc tgtaaagtat ttaccaatat
     4981 ttaaatttaa gctttgacat agttctatta taaagtctcc atatttttta cctaaggaaa
     5041 tataaactac tttctttgta aacagtaaga atttttcttt ttctaataat atatagaaac
     5101 aaattataaa acctaaaaga aattgtacta taaattttcc aactgaaaat gtagtgctaa
     5161 atattccacc aagtgaacca ataagcaagt tactaaattt aggcattaca ctcataattt
     5221 tgtcaccata ctcttttaat gtattagggt caactgattg taaactctta cctaagtcga
     5281 ataagaactg ctctgtttta tctatgtatg taggaatttg agcaactaaa tcagctaaac
     5341 tatttactat ggatggaact gtaaataata taaaggaaat tagtataact atgagtacac
     5401 cataagtaag aagtaacgaa tatattctct taaaatttaa tttactttct aagaacttta
     5461 ctagtgggtt gaatatatat gctaatacaa atgctattat aaatggtgtt agtagtgaca
     5521 ttaataaact tatcactcca aaaaaatatt tgtaattatc aataacttta atcaagatat
     5581 atgaaacaac tacagtgagt acaatattta gataaatttt tttatcttgc aaaataacac
     5641 aacctttctt atatttatta aatttagata tacttaaatc ta
//
LOCUS       DAEMAE010000219         5619 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEMAE010000219 DAEMAE010000000
VERSION     DAEMAE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.221"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      32..134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            228..1604
                     /locus_tag="KPH93_003175"
     CDS             228..1604
                     /locus_tag="KPH93_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG1689948.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1717..2862)
                     /locus_tag="KPH93_003176"
     CDS             complement(1717..2862)
                     /locus_tag="KPH93_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1689949.1"
                     /translation="MNTFEQLKISSTLIEGLKKQDITSPTEVQSLVIGKIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3114..3821
                     /locus_tag="KPH93_003177"
     CDS             3114..3821
                     /locus_tag="KPH93_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1689950.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFISDIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3868..5463
                     /locus_tag="KPH93_003178"
     CDS             3868..5463
                     /locus_tag="KPH93_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1689951.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLVLIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSILETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVAVMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
BASE COUNT         1906 a          648 c          889 g         2176 t
ORIGIN      
        1 atataaatta aaataatatt cgtaaagaaa aattcatgtt ttgtactgta taacatataa
       61 ttttgacaat atgggtcata agtttctacc ggaataccgt aaatattctg actatgtata
      121 ctattattta gttaattaaa tatttaatta actgaattat gaaggatacg gctcagctta
      181 tcaactactg tgtcgttttt ttgtaaaatt taataggagg tgttgttatg caaacaacaa
      241 acagtagtac tatgcagaaa ctttttccaa ttttgactaa taaaaatgtc gacatgaaga
      301 aggaaataat tgcaggagta actacttttt taactatggc ttatataata gctgtaaatc
      361 caaatatttt atctgagaca ggcatgcctg caggtgcact tgttacaggt acttgtttag
      421 cagcagcatt tggatgtatt ttaatgggtg tagttgctaa ccttcctttt gctttagcat
      481 caggtatggg tcttaatgca ttttttgcat atacagtagt tttacaaatg ggtgttcctt
      541 gggaagtagc tcttacagca gtatttgtag aaggtattat ttttatagta ttatcagtaa
      601 gtggagttcg tgaagctgtt gtaaatgcga taccaaaaaa tatgaaatta gctgttacag
      661 gtggtatagg aatatttatt gcacttatag gtctagttaa cagtggaatt gttattggag
      721 accaagctac tcttatcaaa atgggtagat ttacaccagc agtaataata acttgtgtag
      781 gattaataat tattgctgta ttagataaaa aaagagttaa aggttctata ctttttggaa
      841 tagtagtgag ttcattactt gcttgggcat ttgcttttat gaatccagag tatgcacaaa
      901 aattaggtat atatttacct ggtggattat ttaaattcga aagtttagca ccaatagcag
      961 gtaaaataga tttaggatat gtactacatc caacaaatat aggtggtttt ttagtagtag
     1021 tttgtacatt tttatttgtt gatttttttg atacagtagg tacattagtt ggtgtttgtt
     1081 ctaaagcaaa catgttagat gaaaaaggga atgttcctaa tgtaggtaga gccttactta
     1141 cagatgcggt tgcaactaca gttggtgcag gacttggagt atctacagtt acaacttatg
     1201 ttgagagttc aacaggagtt atcgcaggtg gaagaactgg atggacagca ataacagttg
     1261 gaattttatt cttagctgca atgtttttct ctcctgtatt tatagcaatt ccatcatgtg
     1321 ctacagcacc agctttaata tatgttggat atttaatgct tggtacagtt aaggatatag
     1381 agtttgataa tataactgaa ggtgttccag cttttgttac tattgcttgt atggcattaa
     1441 catatagtat aggtgatggt ttaactttag gtatattaac ttatgttttc gtaaatatat
     1501 tttataatat atttggagct aaaaaggcag aagataaaaa acatgtttct gtagtaatga
     1561 ttgttctggc aatattattt ataataaaac tttggctact ataatggtat tatttaaaat
     1621 tttaatttaa tataaaataa agggctgtaa tttattatca attgtgatga taaactacag
     1681 ctcttttgta tttgttgtat tttcgtattt attgtattat ttaataatgt ctattagttc
     1741 tccattttgt aaagttttag gtgtaattac tatattgaac tctttttgca aatcttttat
     1801 aatatctaat tctttttttg ttattattga gattgtgtta ccacttcgat ttcctctagc
     1861 agttcgacca catctatgca ggtattcatt tttacttttt ggaaaatcta agttaaaaac
     1921 atgacttaca tccacaatat ctagtccacg agcagataaa tcagttgtaa ttaaaatctt
     1981 tgctttacca agtttaaatt tatttattgc attctttcta tcttcttttt ccatgtttcc
     2041 aaaaattcct attgctttgt aattatgata atttagtttt gatactagga cttctatatt
     2101 tttttcatta tttacaaata caatagcttt ttttggattt gtagctgcta atgcctttct
     2161 aagaaaagta aatttatccc ttatctcacc taataaatat gagtgattga tgttaggatt
     2221 tatttgtgat ttttcctttg tttttattat ttcaaactcc ttcataagtt tatcacaaat
     2281 ttttatagtg ctatcagtaa gacttgcaga gcatcctata atttgtctat ctcttaacgt
     2341 tgttcttatt atgtcttcta tacaagttat attttttcca ttcaatagat tgtccacttc
     2401 atctaagaca atagttttta tattatgtga ttttagcttc ttttgtttta tcaagtctaa
     2461 aactcttcca caagagccaa ttactatgtg tggttttact gcttttatat tttttatttg
     2521 tttttgtata ttgacctccc caattaaagc taaagaagta acagagagtt ctgcattttt
     2581 agccaataac tcaacttggt ttgtaatctg cattactaat tcatgcgtag gtgcaagtat
     2641 aagagcttgt gtttctcttt ttgatgtatc aattttttca aatataggta aaaggtacgc
     2701 tagtgttttt cctgttccag tctgtgaatt aatcaataaa tccttatttt gaattatctt
     2761 ccctataaca agtgactgaa cttctgttgg agaagttatg tcttgttttt taagtccttc
     2821 tattagagtg gaacttattt ttagttgttc aaatgtattc atattttgct ccttatattt
     2881 aataattata gtcaactatt ttttattata cattgtttat gtaaatcgaa ctatatattc
     2941 ttttataatt atgtgtaatt tgaaagtaaa tatcaattat atttatacct ttctatttct
     3001 gataactttg cattttatag atttctaaaa taaacaaata aaagttgtaa ttgtttgata
     3061 tttgttatat attatatata acaaatatca aaactataat atggagggaa attatgttaa
     3121 aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt agtaagtagt gtctcatttg
     3181 atgtaaatga tggagagctt tttgggttta taggacataa tggagcaggt aaaactacta
     3241 ctattaaagc aattgttgga atacacgatt ttgaagaggg agaggtacta ataaattcta
     3301 aatctataaa aaaacagcct gttgaatgta aaaaggaaat ggcatatata cctgataatc
     3361 cagatttata cgagtcatta actggtatac aataccttaa ttttatatct gacatatttg
     3421 aagttgccaa agataaaaga gaagagttaa taaaatatta ttcaaataag tttgaactca
     3481 ataaagcatt aggagattta atatcttcat attcgcatgg tatgaaacaa aaattagcta
     3541 taatttcggc tttgatacac agcccaaaaa tcttaattct tgatgaacct tttgtaggtc
     3601 ttgacccaaa agcttccttt atattgaagg aaattatgag ggagttttgt gacaaaggtg
     3661 gttgtatatt tttctcaaca cacgttttag aagttgctga aaaaatttgt gataagattg
     3721 ccataataaa agatggaaat ataattgctt atggaactac agaagaagtt aagggaagta
     3781 attcacttga aagtattttc atggaattaa tagagaaata aaatgttttt tatacaatat
     3841 agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa gtatattaat aaaaactaat
     3901 ataataaatg aatttaaatt aaatgcgtta aaaaaagctg atggtagaga aaaacaaaaa
     3961 ataatgggaa tgatgctttc agttgttata atagcagggc tattaatttt ttatgtaacg
     4021 agcttaagcc tttctttagt tgatatttta aagcaaataa atcagatgga aatgctactg
     4081 atatttggat ttggattatc aacattaaca actattgtta catcattata taaaacgtca
     4141 tcatatctgt ttcaagcaaa agatttagac cttttgacta gtttacctat aaaagaatct
     4201 actgtgctag caagtaagat attaatgttg gttggagcaa actatttatt ttcttcaata
     4261 tgtatgatta taccagcagt ggtttatttt tttaattctg atgtaagtat aacgtatttc
     4321 ccttatttga tactgctatt tttagcttta ccattattac caatagttgc ttcatcaata
     4381 atttttatgt ttatagggca gatatcatca agaataaaat acaaaaattt agtattaatt
     4441 ataggtagta ttatattgat gttgctttat actttactta tgacaagact taactctatg
     4501 tcggttgaga tgttaaagaa tagtgtatct attttagaga cgattagaaa aatatatcca
     4561 cctatatatt attttgttga cgctcttaaa acaggaagcg tagtttcctt tttagcattt
     4621 atagcaattt caataatacc attttttatg tttgtaacat tatttgcaaa aaaatttaaa
     4681 gatataaatg ctaagatgag tgaaggatat aagtcaaaaa cttacaaatt taagagccaa
     4741 aaaacttcaa gaccttcaaa agctctatta aaaaaagaat ttaagagata tacttcttca
     4801 tttatatact ttttaaattc atctgcaggt ttgttatttc taatagtagg tacaatttta
     4861 atactattat ttggagcaga taaagtgggt gagttactga agttgaatat agattttaat
     4921 tttataaaaa tacaattatt gggagtattc ttctttataa tagtaatgaa ttgtacaaca
     4981 tactgttcaa tttctcttga aggaaaaaat ttatggatac tgaaatcttc tccaatagat
     5041 gaaagagaaa tattctggtc aaaaatattg atgaattttt tattgaatgc accactatct
     5101 attttatgtc taatattagt atctataaga ttagactttg aaattcagtt tattattgta
     5161 tcaatctgtt tgattgtaag tttttcaatg tttgtagcag tgatgggatt attatcaaat
     5221 ttattatacc caaaccttga ttggaaaaat gaggtggctg tagtaaaaag aagtgcaagt
     5281 atgataatta caatgttagt atcattagct tatgtaatta tacttggagg tatatatgta
     5341 ttacttggaa tttcgttttt aaatatatat attattttgg caagtatact tactttgata
     5401 ttagatttta tgatatggag aattatttct actaaaggag taaagatatt taaaaattta
     5461 taggtgtatt tatgtcaaat aaagacttat cttttttagt cttgcaaata ataggttaag
     5521 tagtatacta tgtatagtga tatgaaaaaa tttaggagaa aataaatgag aaaattatcc
     5581 aaaagagaaa aagattttat tcaatatgga tttttatta
//
LOCUS       DAEMAE010000220         5601 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEMAE010000220 DAEMAE010000000
VERSION     DAEMAE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.222"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            3..743
                     /locus_tag="KPH93_003179"
     CDS             3..743
                     /locus_tag="KPH93_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG1689952.1"
                     /translation="MKDYEDVVYIKLIKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKATAMI
                     EGAGTGAGGIFLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYINSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            952..2103
                     /locus_tag="KPH93_003180"
     CDS             952..2103
                     /locus_tag="KPH93_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG1689953.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRAAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            2128..2883
                     /locus_tag="KPH93_003181"
     CDS             2128..2883
                     /locus_tag="KPH93_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG1689954.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKYIKKVVGTGYGRGVMDFADKKVTEITCHTRGVYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSASMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            3076..5463
                     /locus_tag="KPH93_003182"
     CDS             3076..5463
                     /locus_tag="KPH93_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG1689955.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKVQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSNIIDNFYKKDTLKNAFVVKNGIKSQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANQNAFKELVKLKSAK"
BASE COUNT         2218 a          597 c         1071 g         1715 t
ORIGIN      
        1 atgtgaaaga ttatgaagat gttgtttata ttaaactaat caaaaaagaa cttgaagaat
       61 ggaagaaaaa tatattaaag aaaccttcta tatttgacaa agcatcaaag ggtatacaga
      121 ataaatttaa tggagtattg ccagataaat atcatgaaat aattacttct tctataaaag
      181 taatgacaaa aaccgtattg tttggatata agtatgctac aaagccacca cttagaaata
      241 tgtcattaga agaaagagaa aaattagttt tagaaaaagc taaaacttac aaagccacag
      301 caatgattga aggagcagga acaggggcag gaggaatctt cttaggttta tctgattttc
      361 ctttgctttt aggtattaaa gtgaaatttt tatatgatgt agcttcaata tatggatatg
      421 atgttaaaga ttatagagaa aggatttata tattaaacat attacagttg gctttttcta
      481 gtcaacagca tataaacaaa acttttaaat tgatgaataa ctgggatgaa tatataaata
      541 gtctaccaga agacatcaat gattttgatt ggagaacatt tcaacaagag tatagagatt
      601 atttagattt agctaagttt ttacaactaa tgcctggaat aggagcattt gtgggatttt
      661 atgtgaatgg aaaattaatt gataaattat gtgaaactgc tgtgtctgct tatagattga
      721 ggattcgaga gcttaagtta taaaagttat aagagaagtt ataacataaa aaattagtta
      781 tacaatatat ctatatacat ttaattttgc attgataaaa acaattatgg ataaaaggta
      841 aagcatgata gaatctaatg tagaggtttg tattttaatg gaaaaattta gtttttattg
      901 tttaagatac aaattgaatt atgaagagag gtaattggga ggaatatttt catgacaaat
      961 ttgccaaaac aatttgatag cttcaatgag gctagacaaa aaggatttat aaatgcaaag
     1021 gaacttaagg aaagtggaaa aaagatggtt ggagttttct gtacatttac tccagttgaa
     1081 attccaatgg cagctggagc aactgtagta ggtgtatgtg gtgttagtga agagccaata
     1141 cctgatgcag aaagggtgct tccaagaaat ctttgtcctt taattaaatc aagctatgga
     1201 catgcaataa cagatacctg tccttatttc tatttttctg atttgttgat aggagaaacg
     1261 acttgtgatg gtaagaaaaa aatgtatgaa gaacttgcaa aagttaagcc aacatatgta
     1321 atgcatttgc caaatacctc aaaaggagaa tttgcctata aactttggaa agatgaaatg
     1381 ataaggttaa aagaagaggt tgagaaatct ttaggtgtaa ctataactga agaagatata
     1441 agagctgcta taaaagataa aaatgaagaa agagaacttt taaaagagtt ttatgcactt
     1501 ggtaaattac aaccatctgc acttacaggc cttgagttac ataatgtttt atatcaagca
     1561 ggttttaaat ttgaccgtgc agaacttaaa cattctttaa gaaaagttat agatgatatg
     1621 aaagaaagat atgaaaaagg tgaatgtcct gttcaaaagg ataagccaag aatattgata
     1681 acaggttctc ctattggtgg aataagcgaa aaaatagtta aaactttaga agatgcaggg
     1741 gcatcagtcg ttgcatatga actttgtgga gctattaggt caaacgattt attggttgat
     1801 gaagaaatag aagatgtata tgatgcttta acacaaaaat atataaatat aggttgttct
     1861 tgtatgatga ataatgataa tcgtattgaa ttattagata gaataataga tgaatataat
     1921 gtagatgctg taatagatgt agttttacaa gcatgtcata catttaatat agaaagttat
     1981 agaataagag aatttgttac aaaagaaaaa aataagccat ttatgtctct tgaaactgat
     2041 tattcaaaat cagatactga acaattaaga actagatttg aagcgtttgt agagatgttg
     2101 taggaataaa taaaagtagg aggaactatg tttagtatag gagtggactc agggtcagtg
     2161 gcaacaaagg gtgtgttatt tgatggtgaa aaaattatta agaaaataat tataccaact
     2221 ggatggagcc ctaaaagtac atctaaacag gtctatgagt tgctaagtag tgaaattgat
     2281 aaaaaatata taaaaaaggt ggtaggtaca ggttatggaa ggggagtaat ggattttgct
     2341 gataagaaag taacagaaat aacttgtcat actagaggcg tttacttttt aaacaagaac
     2401 ataaggacta ttttagatgt aggtggtcaa gatagtaaag ttataaattt agatagagat
     2461 ggaaatgtat ttaactttat aatgaatgat aagtgtgctg caggaactgg tcgatttttg
     2521 gagattactt ctaatctttt aggaagcgat attgaaagta tagatacatt agctaaagga
     2581 tatgaaccag ttaatatatc tagtatgtgt acagtttttg ccgagtctga aattgtaagt
     2641 cttctagctc aaaacatttc tactggagag gtagcagcag ggatacttaa atctattgca
     2701 aataaatccg cctcaatgtt agcaagaggt gaggtaatag atgaagttgc atttactgga
     2761 gggcttgcaa aaagtaaaga gctagtaaaa atgatagaag aaatactggg taaaaaaatt
     2821 tttattgcag aagatactca aataatagga gctttaggag ctgctgtaat aggatttaga
     2881 taaaaatata taaaaaagaa aatgaaaagc tgaatttatc ataaataagt tcagtttttt
     2941 attttatttt gtttatttat aaaggaaaac tttggtataa ttgatttaaa tgattgtata
     3001 tttgtaaata cttgatattt agtcatatat aacattagaa ttttaaaaat tagctcaaaa
     3061 taagggggag aaagcgtgaa aaaatttact tcaaaaaaag tagcaaggtt tgttgcactg
     3121 tttttaatat ctattttagt aatgtcaaca attcctgtgt cagctgacaa tagcaataca
     3181 ttgaatcaga taaaaaaagc agaatacagt gatacttata aacaatatct aaatgatgct
     3241 aaaaatggac gtacagaaaa atataatggg attataccaa atccatatca gttggaaggt
     3301 actcaaattc aaagtaaagg aagaacagct ccaacatctt atgaccctag aaaattaggt
     3361 cttatgacat cagtaaaaaa tcaagaggat ttaggaatat gctgggattt tgctgcaatg
     3421 gcaaccttgg aaagtttctt gaagttaaat aattatggag actacgattt atcagaagaa
     3481 cacttaagat ggtgggcttc agatggtgaa tatggatggt ctgtaaatga tatggatggt
     3541 gctttaaatt atgaagctat gggatattta acatcttggt caggtcctaa attagaaaaa
     3601 gatatcccat acaatggtag agtatctaaa gtacaaggag caaaaaaacc aactaatatg
     3661 aatacagcac ctactgtttt taatgtgaca gatgctgtgt gtgtaagtaa tgacataaat
     3721 tctacaaaaa atgctatatt acagtatgga gcagtaacgt ctggttatta tgaagatatt
     3781 aaatatcaaa gtgatgacca aaattcatat tattgtccaa caaaaagttt taatacaaat
     3841 catgctattt caattgtggg ttgggatgat aactattcta aagataattt taatgcaaac
     3901 attagaccat caaaaaatgg tgcttggtta ataaaaaata gctggggaga ttataattct
     3961 gagggtggat atttctggat atcttatgaa gacaagacac ttatgtctga tatagataat
     4021 ttttcaatca aaggtggtaa aaaaccaaat gatgatgaga agatgtatca acatgactat
     4081 gcaagtattg tacctttaat ttcaaaaaag ataacagctg caaatgtatt tgatttcaat
     4141 agaggcgatg aaactctaaa atctgtaatg ttcttaactg aaagtatagg agctaagtat
     4201 gaagtatatt atgctccagt agtaaatggt atacctcaag aaaacaatat gaaaaaatta
     4261 aaagaaggta ctgcccaata ttcaggatac ataacagttc caattgattc ttttaatata
     4321 ccagaaggaa agggtgcaat tgtagtcagt atagagggga aaaatggaga gtcaactata
     4381 ggttctgagt caaatgtacc aggatatgat acatttaaag caaaagctaa tttaggtgaa
     4441 agttatatca tagataatac tggaaaattc tttgatataa atagagatag taacttctat
     4501 ccgtgtaatt ttactataaa agctgttaca gaaaagtctt ctggtgagag tatcccaaat
     4561 gagtctttaa ttggtagtga tagatatgaa actgctatca aagttagtca aaatggtttc
     4621 aattcttcag aaaatgtagt tttagtaaat ggcagttcaa tagtagatgc attggcagca
     4681 actcctttta catctgctat aaattcacca atattattga cacaaaaaga agctttaaat
     4741 tcaaaaacta aagctgagat acagagatta ggtgctaaga aagtatacct aataggcgga
     4801 gaaaattcta taagtaaaga aattgaacaa caattaaaaa gcttaaatat atcgatagag
     4861 agaatatcag gaagtgatag atataagaca agtttattat tagctcaaaa gttaaatggt
     4921 ataaaaaatg tatctcaaat agcagttgta aatggagtga aaggattagc tgatgcaatt
     4981 agtgtaggtg cagcagcagc agaaaataac ataccaatca tacttgcaaa tgaaaagagc
     5041 gaattgcaag gagctgatga atttctaaat tcattaaata tagagaaatc ttatattatt
     5101 ggaggaacag catccctatc aaacaatcta gaaagtaagt taaaaaatcc aactagatta
     5161 tctggtagta gtagagatga aactaattca aatataatag ataattttta taaaaaagat
     5221 actttaaaaa atgcatttgt agttaaaaat ggaatcaaga gtcaaaatga tttaatagat
     5281 ggtttggcag ttggtccttt aggagcaaaa actgggtcac cagtaatttt agttggtgat
     5341 aaattagctg atagccaaaa agaagttctt aaaaataaga ctttagaaaa agtaactcaa
     5401 gttggtgggg gagcaaatca aaatgcattt aaagagttag taaaattaaa aagtgctaaa
     5461 tagggtactt aagtattcac atatactagt gtctattagt aacttatctt aataaaagct
     5521 gaaaataatt actttaaatt attgataaat aagataatca atgattgtaa agttttgttt
     5581 tcagctttaa atttatgtag t
//
LOCUS       DAEMAE010000221         5583 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEMAE010000221 DAEMAE010000000
VERSION     DAEMAE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.223"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(74..2293)
                     /locus_tag="KPH93_003183"
     CDS             complement(74..2293)
                     /locus_tag="KPH93_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1689956.1"
                     /translation="MTGRNKSFTLIIILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAIQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVKNDGTIILHPQGDRLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKNWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIVLIFIAIIFYINYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVNGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCTD
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNILNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLDGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(2564..5218)
                     /gene="adhE"
                     /locus_tag="KPH93_003184"
                     /gene_synonym="adhC"
     CDS             complement(2564..5218)
                     /gene="adhE"
                     /locus_tag="KPH93_003184"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG1689957.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLETVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETRTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEFKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1823 a          993 c          595 g         2172 t
ORIGIN      
        1 actaattatt tagcagtgat ttattaaaat caagcattca taatagtgct ttatataaaa
       61 atcaaatttt gttttaaagt aaatctaaaa aattcttagc tggcattggt cgtgcaaaat
      121 aaaatccttg aactaaatca catccaaatt gtttcattaa ctcagcttgt tctttactct
      181 caacaccttc tataattaca actaaatcta gtgattttgc tagagacact attgattttg
      241 ctatattgcg acttctctca tcatgttcaa taccatcaag aaaagactta tctagtttca
      301 atatatcaat tggcatatct cttaaacaac taatagaaga atatcctgta ccaaagtcat
      361 ccatagaaat agaaaatcca tacttcctca actcatttaa aatattcttt aaaatattta
      421 actcattaat agccacagtt tctgttatct ctatttcaat taaatctgaa cttagattat
      481 acatttgtat tgttttattg ataaacttta caatatctgg ttgatacaac tcaactctag
      541 ataaatttac tgaaactgga actatctttt taccttgctt tttccattca gataaatcag
      601 tgcaaaccct ttcaaataca aatctcccaa tttttaaaat caacttactt ttctctgcta
      661 ttggaataaa tactgcggga cttataaaac catgttctgg atgattccat cttattagtg
      721 cttcactccc tatcatttct ccatttacaa gtgaaaactt aggttgataa tacacttcaa
      781 attgattttt taccaatgct atttttatat catctaaaat cattgattct tcactaagct
      841 tattcctaac atcttcatta taaatagcat aatttatatt tttaccttta gcaacagacc
      901 tagccatatt tgctttattt acagcctttt gaatatctac ttcaccttct tcaacaaaat
      961 aaatacccac acacagagaa atctcaaaga ctatactcaa attactttcg atattatttc
     1021 gtatactatc aagttttctt ataattttat ttcttccatt tttataatca cagaaaattg
     1081 caaaataatc acttgaaagt cgtgcatata cttcaccttc tgttaattct tcttttaaag
     1141 cttttgctat cgtaattaat aaattgtcac caaattcata tccaaaagta tcattaatca
     1201 tcttaaagtt attaatatca aaatatacta taatataatt taaagatggt ttatctaaaa
     1261 gatacttact acactctcta taaaatttat tttgatttcc tatataggta actttatctt
     1321 cataagccaa acttaatatt tctttttcat tagatttttt tatataattt atataaaaaa
     1381 taattgcaat aaatattaat acaataaata acactgcata tatagctctg ttgattattt
     1441 gttctgaatt actaaataca acagtttctg gaattgaaaa aatcaaattc cagtttttta
     1501 tataattatt ttcactattc ccattatcca tagtagcata tcctaaatat cttctttctt
     1561 ccaacatatt tagtacggtt gcacctgttt tattttcttg caattctttt tttaatctat
     1621 ttacttcttg aatatcatta tcctgtttta acaatttata aatatttttt gatagtctat
     1681 ccccttgtgg atgaaggata attgtaccat cattttttac aacataagat atactattat
     1741 tttcataaaa agatacatta ataagtttca tcagagtatt gatattgtaa gtacaatata
     1801 aaactccaac tactttatca ttatttaata atggtactga aatagcaatc ttctttatag
     1861 acttgtttac ttcatcaaca tatggacttg aaacatactt cttaccttta attgagtttt
     1921 tgaaaaattc tctatctttc actgaaaact tatcaccttt ggtattttca gcattaccat
     1981 ttatatcaac tattgccata gtgtaaaatt tttgattttc catttcatct aaaagatttt
     2041 tcattttttc ctcattaggt atatctggat ttgaagcata atagttagaa aaaatattta
     2101 aaacatctaa atctttttca atttgtcttt gtgctagttg agagccttgt attgcaactt
     2161 cttttatatg tatttttgcc tctgacataa gaagatgttt tgtatcttct atatagatat
     2221 accccattgt caaaaataat gatgtcaaaa aaagaattat aattaatgta aaacttttat
     2281 ttctccctgt cattcgactc ctcccctaat tagtcaatac ttcatgtaga taatatatcc
     2341 tttttatacc aattcatcat ttatattgta tataaatatt cttaaatgtt acacataaaa
     2401 tcaacctaat aaccataaaa acattctttt attattataa taacactatt taattgaatt
     2461 ttaaattaaa gaataaaaac tatccatttt ctcaaacaaa aaaactctta tacaataaaa
     2521 ctttgtttta tgtataagag tttttatcta ttttatagta aacttatctt ccataatatg
     2581 aatttaaata catatctttt atttcattca taagtggata tcttggattt gctcctgtac
     2641 attggtcatc aaatgcttga attaccattt catctattga atccataaac ttaaattctt
     2701 caactccaaa ctcttttata gatttaggaa taccaacttt aagttttaaa tcttcaatag
     2761 ctaccaataa actttggaat ttctcttcat cagaatttcc ctttaatcct aaaaatgaag
     2821 ctatttcagc atatctcttt aatgcttctg gatatttata ttgagagaat gctcccattt
     2881 tctttggtgc atctgaagaa ttaaatttca taacttctgt tatcaaaaga gcatttgcaa
     2941 caccatgtgg tacatggtgg aaagcaccta atttatgagc catagagtga caaacaccca
     3001 aaaatgcatt tgcaaaagcc atacctgcca ttgtagaagc atttgccatt ttctctctag
     3061 cttctgggtc ttttgcacca ttatcatatg cacgtggaag atattcaaat atactcttaa
     3121 tagcttgtaa agctaatcca tttgtataat cagttgcaag catagataca tatgcttcta
     3181 gtgcatgagt taaagcatct ataccagaag cagatgttaa gctttttggc atattcatca
     3241 tcatatcaga atcaactata gccatattcg gcatcagttc ataatctgct aatggatatt
     3301 ttactcctgt atcttggtca gttataacag caaatggtgt tacttctgaa ccagtacctg
     3361 ctgatgttgg tatagctaca aaattagctt tttcacccat tttagggaat gtatataccc
     3421 tttttcttat atccataaat ctcatagcta ggtcttggaa atctacatct ggatgctcat
     3481 acaatgtcca cattatcttt cctgcatcca ttgcacttcc tccacctata gatattatta
     3541 catctggatt aaattctctc atagctttag cacctatctt agcacattcc aaagttgggt
     3601 ctggttctac ttcaaagaat gtagtgtgct taatattcat ttcatctaat aaatcagtaa
     3661 ctgctttagt atatccatta ttgtaaagga atgtatctgt tacaataaaa gctctcttct
     3721 tattcattac atctttgagt tcttttaatg ctactcctaa acaacctttt ttgaaataaa
     3781 ctttttctgg tgctctaaac caaagcatat tttctctcct ctcagctact gtcttaatat
     3841 taagtaaatg cttaactcct acattttcag atactgaatt tccaccccaa gacccacatc
     3901 ctaaagtaag agaaggagct agtttaaaat tatataagtc ccctatacca ccttgagaag
     3961 aaggagtatt aattaaaatt ctacaagttt tcatggctga agtaaactta tcaagttttt
     4021 ctctttgatt tacatcatca atatataatg aagatgtatg cccaaatcca ccatcttcta
     4081 ctaatttttc tgcttttcta actgcatcat caaaatcact tgccttgtac attgcaagta
     4141 ctggtgaaag tttctcatga gcaaaagctt cttctatttc aactgattct acttcaccta
     4201 ttaaaacctt agtatctact gggacttcaa agcctgcaag ttttgctata gtacaagctg
     4261 tttgcccaac tattttagaa tttaatcctc catttactaa aattatattt cttatttttt
     4321 ctgtttcatc tttatctaat aaataagcac cacgctcttt aaattctttt ttgacttcat
     4381 tgtatatatt ttctaatact ataaccgatt gttcagaagc acatatcata ccattgtcaa
     4441 aagtcttaga tacaagaatt gagtttacag cagtttttat atctgcactt tcatcaatta
     4501 ttgctggagt attacctgca ccaactccaa tagctggttt tccagatgaa tatgctgatt
     4561 taaccattcc agggcctcct gtagcaaggg tcaaatccac ttctttcata acagttgtag
     4621 ttaattctaa agatggctca tctatccatc ctattatacc ttcaggagct ccagctaaaa
     4681 cagcagcctc aagaacaact tttgcagcct ctatagtaga attttttgct cttggatgtg
     4741 gagagaaaat tattccattt ctagttttta aagctaaaag tgctttaaag atagcagtag
     4801 aagttggatt agttgtaggt acaactgctg caataacacc tattggctca gcaattttag
     4861 tcataccaaa tgcttcgtct ttctctataa caccacaagt tctagtttct ttatacgcat
     4921 tatatatata ctctgaggca taatgattct ttataacctt atcttcagca attcccatac
     4981 cagtttcttt ttgtgccatt atagctagag gaactctttg cttattagct gccaaagatg
     5041 ctgctaaaaa aattttatct acctgctctt gagtatatgt agcaaatatt ttttgagctt
     5101 ctctgatttt tgctagtttt ttaactaagc tttcaacgtt atttacagtc tctaaaccaa
     5161 ctgacataac ttcattttct tttttatttt ctattattac ttgttcttta tgagccatct
     5221 taacaacctc ctgagatagt tttttattta ttatgttaat ttattaacat tatagcattt
     5281 actttttcta ttttcaagtt atttttttaa cattttattt tttatctaat ataacatcat
     5341 ttataccaat aatatctagc tttacaaatg cttagtttaa ctagctatca ctttattttt
     5401 tacgtaaatt actgataaaa tcatctaaaa acttaaggat aacgttattt ttttaacgtt
     5461 tatatttttg tattttttac aaataaaact ttataaaaat gatttttaat atggtcctgt
     5521 atcaagttta aaaataaaaa tacttgaaaa tgaaaataat aacttaatta acaagttaaa
     5581 gta
//
LOCUS       DAEMAE010000222         5583 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEMAE010000222 DAEMAE010000000
VERSION     DAEMAE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.224"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..1670
                     /locus_tag="KPH93_003185"
     CDS             246..1670
                     /locus_tag="KPH93_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG1689958.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKVPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1705..3834
                     /locus_tag="KPH93_003186"
     CDS             1705..3834
                     /locus_tag="KPH93_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG1689959.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKNKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKNKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKTFIDK"
     gene            3864..4286
                     /locus_tag="KPH93_003187"
     CDS             3864..4286
                     /locus_tag="KPH93_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1689960.1"
                     /translation="MSKVLNDNNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4305..5456
                     /locus_tag="KPH93_003188"
     CDS             4305..5456
                     /locus_tag="KPH93_003188"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1689961.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKTRKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
BASE COUNT         2320 a          574 c         1015 g         1674 t
ORIGIN      
        1 gaattattat tttcatttct attaaattta aatattttat aaaaatagta ttatttacaa
       61 aatcatatac tccaaaatac tacataaata aaattaaaca aaaactaaaa tgacaaatgt
      121 cattatctaa agtgacatag aaacggtttc ttaaagttgt tactataaaa tataaactct
      181 ataataaaag tataacaaaa gggaatcata tcgatataga atataattca agaggaggta
      241 aaattatgag tagttctaat caatcgacaa taaagacatc tatacagcga gtagggaaat
      301 ttttaagtgg tatggtaatg ccaaatatag gagcttttat agcatggggt cttataacag
      361 cattatttat accaacagga tggatgccta atgaaaaatt gagtactata ggcgacccaa
      421 tgataaaata ccttttacca ctattgatag cttatactgg tggtaaagca atagcaggac
      481 aaagaggtgg cgtaataggt gcagcagcag ctatgggagt aattgttggg gcagatatac
      541 caatgtttat cggagcaatg ataatgggac catttgcagg atgggtaatt aagaagtttg
      601 ataaatttgt agatggaaag gtaccaacag gatttgaaat gttagtaaat aacttttcaa
      661 taggtataat aggtatgctt cttgcaatac taggatttta tgcaattgga ccagcaatag
      721 tagcagggac agctcttata gaatctggag ttcaatttat agttagcaaa agtttactgc
      781 ctttagtttc agtatttata gaacctggaa aagtattatt cctaaacaat gcaataaatc
      841 atggtatatt agggcctata ggtatagctg aggcaaaaga agcaggtaaa tctataatgt
      901 tcttactaga aagtaaccct ggaccaggtt taggagttct attagcatat tggatgttta
      961 gtagaggaag tgtaaaacaa tcagcaccag gagctgtaat aatacatttc tttggtggta
     1021 tccatgaaat ctacttccca tatattttaa tgaatccagt tttaatattg gctacaatgg
     1081 caggtggagc agcaggaata ttaacttttt caatattagg agctgggctt gtagcagcac
     1141 catcaccagg aagtatattt gcacttatgg cactagctcc aaagggagga ttacttccag
     1201 tattagcagg ggttgcagtg gcaacagttg tatcattctt agtggcagca ccatttgtta
     1261 agagagcttc agcaaatgaa agtgaagaag acagtacctc tttagaagaa gctaaagcaa
     1321 aaatgagtga tatgaaatct gcaagtaaga actctgagaa aaatattgag aaaaaacagc
     1381 ttgaagtaaa tgagattaag aagattgtat ttgcatgtga tgcaggaatg ggttcgagtg
     1441 caatgggcgc ttctagattt aaaaatagaa taaaaaactt agatttagat atagaaatta
     1501 caaattcatc tgttgataat cttccagatg atacacaaat agtagtaact cacaatacat
     1561 tagttgaaag agttgctaag aacaattcaa gtgttgagat agtttcaata aacaattttt
     1621 taaatgaccc aaatttagat acattattta aaagattaga atctaaataa aaataaaaag
     1681 taccactaat taggagtgga taatttgaaa aaaaagaaga ttacatctag gcaaaaaaag
     1741 ataatactaa tgatagtaga aaattcaaag aaaaacattc caataactat ttctgagatt
     1801 gcagggacgt tagagttaag ttcaagaact gtacttagag atatgtctgg aattgaaaaa
     1861 tggtttgatg aaaatgattt taattttgta aaaaaaccag gagtgggatt gattttagag
     1921 gaaaatatag aaaatcaaaa ctttataatt gagttgttag aagaagaaaa aatagaaaaa
     1981 gaatacagca aggaagaaag aaatttaatt atattatcaa agctactagt ttctaacgag
     2041 ccagtaaaat cttattattt tgcaaaaata ttaaaggtat ctgaaggggt actgaataat
     2101 gattttgcat tagcaagtaa atggttggaa agatttgata ttgagcttgt tagaaaacca
     2161 gggcttggtg tctacctaaa aggtcaagaa aaaagcttta gagaggcata tgtaaatctt
     2221 atatacgatt cattcaatga aaaagaaata ctagatatgg tcagaaatat aagtgaaaat
     2281 atacaaacag ataaagcaat agaaatttta agtgaaaata gacttttaaa tttaatggat
     2341 aggtgcataa taagaaaagt tgaagctaca ttaacaaaga agctttcaga tttagatgta
     2401 aatttagctg atagtgcata tatagggctt gtagttcata tttcattagc cttacagagg
     2461 ataaaaaatg gtgaaaatat aactatggat aaagagtttc taaaagaatt atctataaca
     2521 gaagagttta aattagctaa agaaatcgta aaaggtatgg aaacagattt taacatggat
     2581 ataccagtag atgaggttgg atatataact atgcatataa gaggtgcaaa gcaaaggtca
     2641 tcttcaaatc ataaggcttt aaacttggat gatatagaaa taatggaaat tacaaataag
     2701 atgatagatt tggctgaaga tgaatttaaa ataagtttaa aaaatgacga gaggttattt
     2761 aaagacctag caaatcactt aggaccttct ataaatagat tgaatatggg attagaaata
     2821 agaaatcctc ttttagatga aataaaaaac aagtattcat atgcctatga tggagttgaa
     2881 aagatttcta gaattataaa agataaacta aatataaatt caatacctga atcagaaata
     2941 ggatatattg caatgcactt tgcatcagcc atagaaaaaa atcttatgat gaatactaat
     3001 ataaatattg ttgtggcatg tcccactgga ataggtactt caagattctt atcaactaag
     3061 attgaaaata aatttcctaa tttaaatata cttgaaacca tatcagctat aaacatagat
     3121 gaagagtatt taaaggaaaa agatgttgat ttaatagtgt ctacagttga gttaaataca
     3181 agtttaaatt atatatgtgt aggtccattt atgagtttag atgatgaaca gataatcaag
     3241 gaaaaaataa aatctatagc tcaaaataaa ttaattaatt taaatacaaa aaatgatact
     3301 aaaaataaga ataaagtata tgaacagata acagaaagta tgaatatagg aaaagatatt
     3361 cttcaatttt tagaggaaat tagatttgaa aaatttgaaa gtaaagattt aagtgagctt
     3421 attgaagact catcaaaaat atttgcaaaa tctagtgaag atataatgtc tataaaagaa
     3481 tcgttaaaag aaagattaaa aatctcaatt ccatatatag aagaatcaaa aattctattg
     3541 ttacattgta tgagtgaaag aatagatatt atgaagttat caataataaa actggagaat
     3601 cgaatcgtgc tagattcaaa tgaagaaatc gacaatgtag tttttatgtt acttcctaaa
     3661 aattcaccaa gttatcaaag acaaataatg agtgaaataa gtggttcatt aatagacaat
     3721 ataattttta ctaataagat aaataaattc tctatagaag agatgacatt agaggtaaaa
     3781 gatatagttt ttaattttta tacaaacaga ttaaaaacat ttatagataa ataaaattaa
     3841 tattaaaaag gaaggttgat actatgagta aagtattgaa tgataataat atatttttag
     3901 ggttagatag tgtttcaaag gaagaagcta taactttagc aggaagaaag ttagttgaaa
     3961 atggatatgt aaaagaagaa tatataccag caatgctaga aagagaaaaa gtaatgacta
     4021 catatatggg aatgggagtt gctattccac atggtgtaaa tgaagcaaaa aaagaaatat
     4081 tatcttctgg aatagttata ctacaattcc ctaatggtat agattttgat ggagaaaaag
     4141 catacttatt aataggaata gctggtgttg gagatgaaca tttagaaata ctatctaata
     4201 tagcagtagt tttagatgat gacttaacag aaagactaaa aaattcaaat gacaaacaag
     4261 cttttatgga agcttttgcc aactaatctt aggagggatt taaaatgaaa aaggcaatcc
     4321 agtttggagc aggaaatatt ggaagaggtt ttattggagg cttgttagtt aagtcgggtt
     4381 atcatgtagt atttgctgat gtaaatgagg agatattaaa ctcaataaac aaagataaaa
     4441 agtatactat acacataaga gatgtagaat gtatagatga agtaattgat aatataagtg
     4501 cggtttcatc tataaaagaa gaaataatag atgagattgt acaggctgaa ataataacta
     4561 cagcagttgg acctttagtt ttgactaaaa tagcctctac aattgcaaaa ggtataaaga
     4621 ctagaaaaga aaagggatta acaagtaatc taaatataat agcctgtgaa aatgcaatat
     4681 acgcaagttc ttctttaaaa gaagaggtac ttaagtattt aaataaggaa gaagtagaat
     4741 atttagagat atatgttggt tttccaaact gttcagttga tagaatagtt cctcctggta
     4801 aaaatgaaaa tcctcttgat gtaacagttg aaaattttta tgagtggaat gttgaaaaac
     4861 aaggatttaa aggggaaata ccaactatag ttggaatgaa tttagctgat aaccttatgg
     4921 cttatataga gcgtaaattg tttacactga atacaggcca tgctataaca gcttatatag
     4981 gatatttaaa aggatataag actatagaag agagtataaa agataaattt atctgtgata
     5041 tagtcaaaag tgcaatggta gaaagtggag aaggattgat taaaaaatac aattttgact
     5101 cagaagcgca ctataaatat attgataaaa ttttaaatag atttaaaaat ccatatctta
     5161 atgatgatgt attaagagtt ggaagggaac cattaagaaa actaagtgat aaagatagat
     5221 tgattaaacc tttaatgaca gcaaaatcat atggattatc agttgacaat ttgattcttg
     5281 gaataggtgc agcgcttcat tataataata gtgaagatac tcaaagtgta gaattacaag
     5341 agttgataaa gtctaatggg gtaaaaaaag ctgttgctaa aatagctaat ataagcaatg
     5401 acgaagaact tttaaataat atcgaaaaaa gttatatatt tatgaaaaat ttataaattt
     5461 acttgaaaaa aagtagttct gataaagtaa ctataatata ttattctaca cagtttgaat
     5521 tatttgttga ataatagaga attataatat ataattaaaa ggaataaatt aatatattta
     5581 cct
//
LOCUS       DAEMAE010000223         5545 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEMAE010000223 DAEMAE010000000
VERSION     DAEMAE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.225"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..1069
                     /locus_tag="KPH93_003189"
     CDS             152..1069
                     /locus_tag="KPH93_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG1689962.1"
                     /translation="MKNIKVGMIGLGSIAQKAYLPILTKSERFKFVGAFTPNKVKREK
                     ICSDYRIIPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEESGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPSISGEECVKAQRLLEKIVSSVK"
     gene            complement(1191..2120)
                     /gene="pfkB"
                     /locus_tag="KPH93_003190"
     CDS             complement(1191..2120)
                     /gene="pfkB"
                     /locus_tag="KPH93_003190"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG1689963.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELKKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNKDSVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            2390..3115
                     /locus_tag="KPH93_003191"
     CDS             2390..3115
                     /locus_tag="KPH93_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1689964.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3198..4352
                     /gene="nagA"
                     /locus_tag="KPH93_003192"
     CDS             3198..4352
                     /gene="nagA"
                     /locus_tag="KPH93_003192"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG1689965.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFDEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNKVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTKYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGYHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLSSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(5048..5530)
                     /locus_tag="KPH93_003193"
     CDS             complement(5048..5530)
                     /locus_tag="KPH93_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1689966.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT         2046 a          714 c          757 g         2028 t
ORIGIN      
        1 tagatatcct agaaaattac tttattaata tttctcattt tcctaattta cttatataag
       61 ctttacattt atttttgata caaagtttac aaatttatgt gtctgaatta taatcaattc
      121 tgtaaatata aaaaccttag gagaaagtat tatgaaaaat ataaaagtgg gaatgatagg
      181 tcttggctcc atagctcaaa aggcatattt acctatatta actaagtctg agagatttaa
      241 gtttgtagga gcttttactc caaataaagt taaaagagaa aaaatctgta gtgattatag
      301 aatcatacca ttcgattcta ttgagtccct agctaaaaaa tgtgattgta tttttctaca
      361 tagctctaca gaaacacact acgaaattat aaaaatatta ttaaatttag gtgttcatgt
      421 atatgtagac aaacctcttg cttcaacagt aaatcaagga gaagaactta tcgagttaag
      481 tacgaaaaaa aacttaaatt taatggttgg attcaaccgt agattctgtc ctatgtacaa
      541 agaaattaaa aataatgcta cagaaatcgt atctatcaat atatgtaaac atggattaaa
      601 ttccttaaga aatgttagat tcgacagtac tcttatagat gactatatac atgttattga
      661 cactgcttta tggttagcaa atgaagatgt tgaaattagt ggagaagatt tatttttaac
      721 agacaataag aatcttatat ttgtaagtca taaacttaaa ggtaaacatt tctctataaa
      781 cacctctatg catagagatt ctggaacaaa gttagaacaa gttgaaattc tttcaaaagg
      841 taagattcaa agagttaaga atcttaatgt actggaaata gaagaaagcg gaaacctaac
      901 tttaaaacaa agtggtgcat gggtaaatat attaaaacaa aagggttttg aggatatatc
      961 aaatcatttt attgattgta tagaaaataa tataaaacca tctattagtg gagaagagtg
     1021 tgtcaaggct caaagattac tagagaaaat tgtaagttca gtaaaataaa tatttattat
     1081 aaaaataggt taaagatgaa ttctctaacc tatttttata taaaactata tcttttatat
     1141 taaaatctat tacaataaca ttaaacttat tatattaaca ttaaatgtat ttaacttaac
     1201 ttttttactt caactaaatc ttgatattta tcaatttctc ccaaatctac aaatccagtt
     1261 tccatattta aagcattact tataccacaa gcattcgcaa atttaagtaa attttctatt
     1321 ttataacccc ttaaaatacc tacactaaat ccagccactg tgctatctcc actacctaca
     1381 gtatttatac actttacaac tggtactttt acatcatata cactatcttt atttaaatac
     1441 accataccat cttttcctaa agatatacat acattctctg cacccatttc aataagtttt
     1501 tttcctgaca agataatttc ttctcttgat tctatgttta ttcctaataa atgtcttatt
     1561 tcatctatat ttggctttat taaatacggt tttgatttta aagctatttt taatgcttct
     1621 cctgatgtat ctaatataaa ttttatatta tttgctctac atatattgcc tatcttttca
     1681 taatagtcta tcggcatatt ctcacaataa ctaccagaag ccactagtat tttagtattt
     1741 tttagtactt cctctatatc attttcaaat ctctctaaat cgctcttttc tactattggt
     1801 cctttttcta aaaattctgt actaacttta ttgtcctcaa ttatgtttaa gcaatttcta
     1861 gtttcctgct ctatttttgt aaacttattt tgtatatcta gttttttaag ttcatttttt
     1921 ataaattctc cattaaatcc acctagaaat cccatagcta aaggattttc ttttaaaatt
     1981 ttacatacct tagctacgtt taatccttta cctccagcag ttgcattctt acttactacc
     2041 ctttgcacct cacctatatt tatattattt actctataca ttctatctat agatgggtta
     2101 aatgttatta ctgtaatcat actagattct cctcattatt aatttttcac ttttctaaac
     2161 aataaattaa tctgtaaaat aaaaaatact tcgtctataa tatactcaaa gtggttatta
     2221 ccaatttgaa tataaattat tttaattgtt ttgtcaatgt atcataattt tataataaat
     2281 taatgacctt acttattgaa ttttgattaa actttattca ttgacataaa taattctata
     2341 ttctataatc aaattgtggt aataaccact tagaaaggac tatattttta tgataaaaag
     2401 aaatgataaa agaccaatat acgaccaact tgttgagata ttaagaacta agattgaaaa
     2461 tgagatggaa ccaaacgata gaatgctttc cgaaagaaaa atttgtgatg aatatggagt
     2521 aagtagaacc acagttagac tagctatggc tgagttggaa catatgggat atatatacaa
     2581 aagacatgga aaaggaactt ttgttgctgc actaagtcaa aattctcaaa acttaatgga
     2641 aagttatagc tttacagatt atatgagaac acaaggaaaa aaaccaggta caaaagtatt
     2701 atcatttgag gtagtagaaa gtacaaagta tttttctgaa aaattaggta taactcctgg
     2761 tgaaaaaatg ataaaaataa tgcgtttaag acttgctgat gaccttccta tgatgcttga
     2821 aagaacatat ctccctatga aagaatttat aggattgaca aaagaaaaaa tcgaacaaaa
     2881 acctttatat gaaatattta gagaagatta tggtgagatt attaaagttg ctgatgaaga
     2941 attttcagct ggaataatct ctgataagga agctaagtta ttggaagtac caggagattc
     3001 accttgtttg aaattacttc gtaccacata taatgatgac aatatagtta ttgaatttac
     3061 tttgagtgtt gctagaagtg ataaatttgt atatagagta agacatgtaa gatagtaaat
     3121 taaatagctt tcaagaaaac agaatgatat tataaacgtc aatgccacaa tgatagtata
     3181 attataagga ggttattttg aaaagtttta tttatgcaga taaatttttt atgaaacatg
     3241 gaatcaaaga aaaaggttac cttagtataa ttaatggcaa attcgatgaa tttcaacaag
     3301 aaagacctaa tattactgat aatgttatag atttttcagg taaatatatt gctcctggct
     3361 tagttgatac tcacattcat ggtcttttag gtgcagatgt catggacaat acatttgaag
     3421 caataaacac aatttcaaaa ggcttattaa aatatggagt aacatctttc ctacctacaa
     3481 ctttaactga ttctgtagat acactaagtg attctgtaga aaatataagc aatagtttta
     3541 ataaagttga gggagcaaaa atacaaggga tttatttaga aggtccgttt tttacagaaa
     3601 aatataaggg cgctcaaaat gaaaaatatt ttaaagaccc tgacatcaat atattaaaat
     3661 tatggcaaag aaaatcgaaa aatttaataa gaaaaatagc aattgctcca gaacgtaaag
     3721 gttcaataga attaacaaaa tatgcaacta aaaataatat ctctgtttct cttggccata
     3781 gttcagctac atttgaacaa gttaaacaag tcgttaattc aggtgctaaa gtgtttgtac
     3841 atacatataa tgggatgagc cctttaaatc atagagaacc tggaatggtt ggagctgcta
     3901 tggtgctaaa agatacttat gctgaattaa tttgtgatgg ttatcatgta agcccaatag
     3961 ctgttaaaat aatgatggat gcgaagtcta gtaataacat agctttaatt acagattgta
     4021 tgagggctgg tgctatggag gatggaaaat atactcttgg taagtttaat gtaaatgtaa
     4081 aaaatagtgt tgcaagatta agtagtggta gtttagctgg aagtgtatta actatggaca
     4141 aggctgtgag aaatatagtt gattggaaca tatcaacttt agaagatgca ataaaaatgg
     4201 ctacatatgt ccctgcttta agttgtaata ttgataatgt ctgtggctct atatatcaag
     4261 gtatggatgc agactttata gtaacagata atgactttaa tatacacgaa acttatataa
     4321 atggtatatg taagtacaaa gttaataaat aggttcaata taaggttatg gaggctgtct
     4381 aaaaagagtc tggaaatatt taaagttatt tctccaaaat ctgatttaac acttttattt
     4441 ccatatcttt aatatattat tgcgatagag gttattttaa acaaatatat attataagta
     4501 tttttgtgat aatattttcg ataatatgta aatttaaagt aacttttcta ttatttgtaa
     4561 gtttttatct tgagcaaaca tataaatttt taataaagca tgagataaac cctatatttc
     4621 aattactaat gatgagattt tgattgataa cagatatatt ttcaaatgta aaaatactaa
     4681 aaatcatatc aattttaaag atattgtagg tgtaaataaa caagataata ctatagaaat
     4741 ttttacaaat gaaagcactt attgtatata tctaaaaatt ttaagcattg agggcgaaga
     4801 atatctaata gatagactaa aatcattttg ttgattttat atattttatg ttaaatataa
     4861 agcagagtag ttaaaactac cctgcttttg taattaaaat ttaaaaataa taactcataa
     4921 tatattgaat ttgagcttaa cagtaactat tgtaataata gcatcaatct tttgtgttga
     4981 atataattta tttacactat agttaagcat gatttctaat gttattttct ctaatttaca
     5041 attatgatta aaattcatta atatatatat atttataagt tgtattgttt atattgttta
     5101 ttactttttt aaatccacta gctgtaataa taaatgtctt ttctggtatc cctggtgaaa
     5161 agattatttt atcatggtta aatatatctt gatctattac aagagagata tttttctcat
     5221 atccaaaagg agatacacat ccaggcaaac attgtgtttt ttgtgttaat tcttcttttg
     5281 aacatataga tacttttcca cctaaaacat tttttatagt ttttggatct aatttttttc
     5341 cttctgtggt tacaaatata tagtaatttc cagacctatc ttttataaat aaacttttac
     5401 tttctactcc agtatattgt aatttttctc ttatttcttt agctttttca aatgataaaa
     5461 tgggttcatg gtcatattct tcaaacgtta tatgattttc ttttaaaata tttttgatat
     5521 tgttgtacat atattttcct ttcta
//
LOCUS       DAEMAE010000224         5545 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEMAE010000224 DAEMAE010000000
VERSION     DAEMAE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.226"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            272..2014
                     /locus_tag="KPH93_003194"
     CDS             272..2014
                     /locus_tag="KPH93_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG1689967.1"
                     /translation="MVKSKKKIFVSLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICKNINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTISKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQKEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDTVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFNLEEEYGVSNNILKEKNAPFWVLVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2178..2372)
                     /locus_tag="KPH93_003195"
     CDS             complement(2178..2372)
                     /locus_tag="KPH93_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689968.1"
                     /translation="MKIITIGSGLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2369..2950)
                     /locus_tag="KPH93_003196"
     CDS             complement(2369..2950)
                     /locus_tag="KPH93_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1689969.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCIANGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            3118..3936
                     /locus_tag="KPH93_003197"
     CDS             3118..3936
                     /locus_tag="KPH93_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1689970.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IENTIKRIGGNDIMKNYDVTIKNFASKYMMTLRMVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKHSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKAVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYEVDGPMFNIYHVSPGNDNNPDNWVTEVCFPVKKK"
     gene            4808..5374
                     /locus_tag="KPH93_003198"
     CDS             4808..5374
                     /locus_tag="KPH93_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689971.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVRINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFD"
BASE COUNT         2114 a          719 c          735 g         1977 t
ORIGIN      
        1 tttttatata tatcttttaa tttaaattgc accaaaaaca tccatatttt ttacatataa
       61 taggtctaaa actatcatag cttttactat tttgtactta aaatttattt atcattttac
      121 cagtataaat ttcatatact tttttgaagt taaatttttt atattattta taatcttatc
      181 tatatcttta tgttaaattt tcttaacatg cattttttat atttaatata gtaaaataat
      241 taataactat atccatgatt ggaggatttt tatggttaaa tccaaaaaga aaatatttgt
      301 ttctctaatt ttaattgcag tttttattat atttgcatca tacataagaa attatactaa
      361 tgatacaaaa aaagattctg ttagaggaac tattcctttt gaaaaaatgg aatataagcg
      421 tcctaatata cagtcaattt gcaaaaatat taacaagtgt aacgacaaac ttttaacagc
      481 taaaacagct aaagaacaat taaatttatt taatgaagtt gataaaattt atcaggattt
      541 ttacagtaca cttactatat caaaaattag aaataacata gattcatcag acgaatttta
      601 ctctaaggaa tataagtatc ttatgagtaa atcagtagat gttgacatgt catacaaaga
      661 ctttcaagat agatttgtaa cttctaaatt ctctaaagaa ttagaaaaag aacttggaaa
      721 ggatacattt aattatctaa agaacattgg aaaacttaat tctaaagaag ttgaaggttt
      781 gcttaaaaaa gagcaagacc tagttgtaaa atatgaagac ttattagcta aatctacagt
      841 atcaatagat ggtatagagg tagactttga agaagccttg tcaagaccaa atcttagccc
      901 tgaagaatat gtgaaaattt attcagatta cttaaaaaaa tacaatccta tatttggtaa
      961 catattctta gaacttatac agacaagaac tgaaatagca agtaagcaag gatttaagaa
     1021 ttatatagat tatgcctata tgaacctaaa caaagactat tctcaaaaag aagctaaaaa
     1081 atttagacaa gatgtaaaag attacatagt cccactatat agagagattt catctaaacc
     1141 ttctgattct tctatttata ttaaagtata taaaaatcgc tcatttagga agtttgatac
     1201 ggtacttgaa gatatatctc caaaactaaa agagagtttc gactatatga aaaaatatga
     1261 cttgtacgat tattcttctg gaaaaaataa atcgcctgga ggatatacta cttatataaa
     1321 taaatataaa gcacctttct tgtttaatac atgggacaat tcttttttag gtgttactag
     1381 ttttgctcat gagtttggtc atttttataa ttactacaac agtattaact taaataataa
     1441 gatgcaacca agtatagatg tatgtgaagt tcattcaaca tctttagaaa ttctatttta
     1501 caaatatttt gatgattttt ttggtaaaca atctgaagct ataaaaaaag aacacttatc
     1561 gattgtttta aatactatta ttgatgcttg tctttatgat gaatttcaag aaattatata
     1621 caagaatcca tatatgagtc taaatgatat caataaattg ttttttaatt tagaggaaga
     1681 atatggagtt tccaataata tacttaaaga aaaaaatgct cctttttggg tacttgtatc
     1741 tcataacttc caagttcctt tttattactt gagttatggt cttgcatctg atgtatcact
     1801 tcagatatgg caattatcac aagaagatta cagaaaagct gttgatgtat atatggattt
     1861 tttaaatcaa aatactgatg ctggatttaa ggatgtggta gaaaaggtga atttgcaatt
     1921 acctttccaa gatggaaatt tagaggaaat aagctctact ttatatgatt attttggtat
     1981 agataatcca cttgaactaa aaaatgcttc ttaattttta atactttatg ctcactctta
     2041 agatttagta ttagattgaa ataagtgata ttttttcaca cacaattaat gtttaataaa
     2101 attatatggt aaagtatcag aaatttttaa gactcttgtc tttataattt ctgatacttt
     2161 atctattaat aatattatta tttttttata tatataatta aaaggattgt ggaaatcaat
     2221 aaaaatatcc ctgtaaaaat agcactattc atatggaact taatagaact tgcatctgtt
     2281 accttattat tcttaatcca gaacccacaa accatagttg aaagaaaaag taaaacagtg
     2341 ataagacctg aaccaatagt tataattttc atcttacatc aacatcctct ccaataatat
     2401 tttctattaa aatatcaata aatttatctc gctcatattt attttttata tttataccac
     2461 tgtataactg aaatgattct gtagataaag cagaaatctg tattggtaaa ataatttgca
     2521 gggcaataat tcttagactc atttcatctt ttttatttcc aaaaatttta cgaagcatag
     2581 ggacaatatc aagttctgct tgcatatctc cattggctat acattgattc aacacaaaag
     2641 gaagcacttt ttcataatgt aatccagtgt cacacaattt ttttaataaa tttttcaagt
     2701 cttcaatagg tcttagagta tatacactat cgtcatatag ctctgctatt atatttgaca
     2761 tcacatctcc tatggcagta ctcaacaaat tatcctttgt tttaaaatga taatttatta
     2821 aaccaactcc aacatttgca cgctcagcaa tttttctaac tgtaatctcc tcaatatcat
     2881 ctacttcatt taaaagattc aatgtaactt caattatacg ttctctagca tctttatcat
     2941 tacttttcat aatagacctc caaaacaatt aacattttca gttctgaaca cgttcagtat
     3001 aatatgtttt tcatcatatg tcaatataat ttaattataa ttttagttga ctctaacatt
     3061 atgatatagt ttatattaat attaagctaa aagtaccttt ataaaggaga caaaaacatg
     3121 tttaagatag gcgatttttc aaagctaagt aaaattagta ttcgtatgtt acgtcattat
     3181 gacgaaatag ggcttttaac tccttctcat acgaataaaa ctaatggata tagatactat
     3241 agtgcagacc aactttctac tacaaacaga atacatgcac taaaagatat gggctttggc
     3301 ttgtattcta taaaagaaat tttgacagaa tataacgata aagaaagtct tataaaatat
     3361 ctaaatatcc accattctca agtaaaagaa caattagaag atacacagaa aaaactatta
     3421 aaaatagaaa acactattaa aagaattgga ggaaatgata ttatgaaaaa ttatgatgta
     3481 actataaaga attttgcatc aaaatatatg atgacactta gaatggttat accaacttat
     3541 caagacgaag gaatgttatg gcatcaagca tttttagaga caaaagacca gaatgttcaa
     3601 attgaaccac caaagcattc taaggcagtc ttttatgata ctggatataa agaagattat
     3661 gtagatgttg aagtacaagt tgctgtatct ggaaagtata aagatactga gcatgttaaa
     3721 tttaaggctg ttccaagtgt gactgctgca actgcaattg ttaatggaaa tttcaatcaa
     3781 gttgcagatg cttgtgaagc tattggtaat tggatatcag ataataacta tgaggtggat
     3841 ggtcctatgt ttaatattta tcatgtaagt cctggcaacg ataataatcc tgataattgg
     3901 gtaacagagg tatgcttccc agttaaaaag aagtaatttt atcttatata ttaattggat
     3961 aatattttaa tttgttagac aaattacttt tagtgttatg tataaatacc tacaatttaa
     4021 ttaggatatt atcttaattt gtcagataaa tcacttgtga tgttatgttt aaatacctac
     4081 aatttaatta ggatattatc ttaatttgac agataaatca cttgtgatgt tgtgtttaaa
     4141 tgactagaat tttattagga tattatctta atttgtcaga taaattattt ataatgttat
     4201 gtttaaatac ctacaattta attaggatat tatctcaatt tgtcagataa atcaattatg
     4261 attttatgtt taaatgacta gaatttgatt aggatattat cttaatttgt cagataaatc
     4321 aattataaga ttatatacta tccttatact ctagttaaat atattttaat cactaatata
     4381 aattagtttt tttaacaaaa caataaaaga gtaaagtaca aataattcct ttgtgctcta
     4441 ctctttttaa ctttctttag acaatgatac aatgataaat ataaaaaatt atatattcca
     4501 ttctatagaa atatgctcac caataacatg tatgctgtta atcaatatat tatatcaaaa
     4561 ttacataaaa cttataggac aatcatatct tattacaaga aaaatgtctt atccatattg
     4621 aataagtcat tcagttcata tacaaagtca atttcattga gaaacattta atatatgttt
     4681 ataaaaattt catactattc tttcatattt gcatgtgata taatttatta actcataatt
     4741 ttgttaatca gttgtgcttg tagattattt atataatcta atattttaag aaggtggtgt
     4801 aataactttg aaattaaaaa ttgttggtag tggcggtatg tcaattattc caagttcatt
     4861 ttgcaaatgc aaaatatgtg aagaagccag acaaaaaggc gggagatatg aacgattagg
     4921 gccaagttta tatattgatg atataaaaat gctgatagac acacctgaag acattgcagt
     4981 agcttgtaac agacaaaaaa tatcagaagt aaaacatatt tcaatttctc accatgaccc
     5041 agaccatgtt aagggtatta gaattgtcga aaaaatagga tgtgacttta tcacaggaga
     5101 aaataatcca attggctttt atgcgcttcc tgaagtaatt gaagatataa accgaacgaa
     5161 tctcgattgt cttaaatatt atggtgatgt tataaattgc atttctatta atgaaacttc
     5221 acacgtaagg ataaataatt ttgatattga tttgattaac aataatccag acaggaatat
     5281 aaccttctat gttatcaaag agaatgaaaa aaaagttata tacgcctgct gtaatcccaa
     5341 gccatttact catagtgata tgtactttga ttaaaaaata gctacgcaca cttgtgactt
     5401 tagtcatgag ttagtagcta aattagtcag catataggga aacttatatg tagagatagg
     5461 atagaaccta tccaatacta cttgaattgc taaaaacccc taaagctaat caaactaaaa
     5521 cataaggatg aaataaacct aagtg
//
LOCUS       DAEMAE010000225         5528 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEMAE010000225 DAEMAE010000000
VERSION     DAEMAE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.227"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            330..1166
                     /locus_tag="KPH93_003199"
     CDS             330..1166
                     /locus_tag="KPH93_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1689972.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVEREKQGIKLPNLILNETKQLYREEFEFGLWAIDVIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1519..3096
                     /locus_tag="KPH93_003200"
     CDS             1519..3096
                     /locus_tag="KPH93_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1689973.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMIPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAALYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGKSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEVCND"
     gene            3089..4273
                     /locus_tag="KPH93_003201"
     CDS             3089..4273
                     /locus_tag="KPH93_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1689974.1"
                     /translation="MIDFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRSSVANWYIKRFNCSVDSDWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRDGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKDNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDNCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSHLKIS
                     SEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKI
                     TL"
     gene            4291..4890
                     /locus_tag="KPH93_003202"
     CDS             4291..4890
                     /locus_tag="KPH93_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1689975.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKSIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4914..5384
                     /locus_tag="KPH93_003203"
     CDS             4914..5384
                     /locus_tag="KPH93_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1689976.1"
                     /translation="MFKIFKKKSIKSPISGKVIELSKIQDVVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIAVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSIGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2083 a          574 c         1043 g         1828 t
ORIGIN      
        1 agctataatc tgaatttcaa taaatatatg tataattaag ttgaaatact tgaaaatgct
       61 ggaaaaaatt tgaaaataat ttttgtaata taaagcgtcg attaatttta aataattaaa
      121 tatacataag aaataattaa atatatttta atgaaaatat gctaaaatat gacaaattaa
      181 ttggtaacaa taaaaaatat tttgttattg tagtattata gggtaaattg caaaatatat
      241 acgaaagtaa aattttaaat attgacatta aattaaatat actatactat gtaagtgtga
      301 tgagagaatt tttttagagg ggatttatag tgaatataaa aaagatattt aataacaatg
      361 tagtagtaag ctccctagaa gatggcacag aaataattgt cactggtgct ggagtaggat
      421 ttaagaaaaa agtaggcgat ttgatagatg aaaatttgat ttctaagaaa tattttgttc
      481 aagacgatca aagggacaaa tacaatcaaa tacttaataa gacctctatt gaatacttta
      541 aaatttcaga agaaattata gaaaaggcta acgaagtttt aaatactcaa gtgaatgatt
      601 caataatatt agccttaact agtcacatag aatttgctgt cgaaagagaa aaacaaggta
      661 taaaattacc aaacttaata ttgaatgaga caaaacaact ttatagggaa gaatttgaat
      721 ttggattatg ggcaattgat gtaattgaaa aaaggatagg aataaaattg cctgaagatg
      781 aagcaggata tattgctatt cacataatca atggtttgga aaattctcaa aaagatgaag
      841 gtattaatat actagagttt tcaaaacaag ttattcagat aattgaagaa gtacatggat
      901 ttaaattgga tgtaaattca ttaaattata ctagacttat aactcactta aagttttttg
      961 ttcaaaggat tctccgaaaa gaaacttaca atgatagtga tattgaagat atgtacaagt
     1021 tagtgaataa aaattacaat aaacctaaag tgtgtactga agaaattgca gcattggtat
     1081 tagacagatt tgaatataag attagtaaag aagaagaatt atatttaatg attcacataa
     1141 ataaaataac aaacagtggt tgttaggatt gtaactgata aagcaggcaa aacctaagtc
     1201 attgagctta actattagat taagtttttt gatttaggtt tttttattag atacaatcaa
     1261 aatacagaca gtgacttttg ggattgtaac tggttaagca ggcaaaacct aaatcattga
     1321 atttaactat taggttaagt tttttgattt aggttttttt attagataca atcaaataca
     1381 gacggtgact tttaggattg taactggttg agcaggcaaa acctaaatca ttgaatttaa
     1441 ctattaggtt aagttttttg atttaggttt ttctataaat tcgattatgt ttttagataa
     1501 aaataggagg ggaaaattat gaaaaaaaat aagttaggtt tatggaactt cttccaaatg
     1561 ttaggaaaaa cgtttatgtt cccaatagca ctacttagtg tttctgggat gatgttagga
     1621 cttggggcag gttttacaga ccctaagatg attgaaatga ttccattttt agggaatgaa
     1681 tatgttaact tagttttaaa cttcatgtta acaataggat tatttgcatt taacaacttg
     1741 ggagcattat ttgcaatggc aattccacta ggattattaa aaaaagaaaa agagtttggt
     1801 gctttttcag gattagtagg atttatagca atgcatatag gtacaaattt ttatttaaca
     1861 agagctgatt tgttagtacc agcagaacag atgtcaacta atggtcaagc tatgattatg
     1921 ggtatacaaa catataatac aagtgtttta ggtggtattg tagcaggatt aattgtttat
     1981 gcaatatatg ataaagttag taatttaaag ataccagaat cattaggatt ctacagtgga
     2041 ccaagattag ttccaattgt tagtttaata gttatgagta tagttggatt attaatacct
     2101 attgtttggc caccattttt caatgcattc caaagtttag gaagatggat ttcttcagca
     2161 ggaccattag gatatttttc atatgcagtg gcagaaagag ttacaattcc atttggacta
     2221 aatcatcttg taacatctgt attccgtttt acaccaatag gtggaactgc tgttattgat
     2281 ggagaaacgt actttggtac attgaacatg tttatggcat atgtagaaaa taatcagatt
     2341 atacctcttg atttagctgg taaaatggaa caaggtaaat tgatgattca atatggatta
     2401 gctggtgctg cattggctat ctaccgtaca gcaaaaccag aaaatagaaa ggcacttaaa
     2461 ggacttttaa tatcaggagt gttgacagtt attataggtg gtataagtga accaatagag
     2521 tttctattct tattcatatc accagcatta tttgctttcc atacatttat gaatggtcta
     2581 gcaaacatgg tacttccata cttaggcgtg ttgatgggat tcacaggaga tttaattgca
     2641 tttataagtt ttggtgtgtt aagaggaaca gctacaggat ggccaatagc agtattagta
     2701 gcagctttat actttgcaat atactatttt gtatttaagt gggcaattat taagtttaat
     2761 ataaagaccc caggacgtga ggataaagaa ataaaagtat ctgaaggaaa tggttcagat
     2821 tttaaaaatt tgtctacata taaaggacaa caaatgatat ctgctttagg tggtaaatct
     2881 aatatattat cactagataa ttgtgtaact agattacgtt taaaactaca agatgtatca
     2941 ttaataaatg aggatgcaat taaagaggct ggtggtatag cagttgttaa attggatgag
     3001 catacaatac aagtaattat aggaactcaa gtgtattctt taagaaagca gatggataaa
     3061 gctatggaag gtcatttgga ggtatgtaat gattgatttt gataaaaaag tagaccgatt
     3121 gggtacatat tgtacccaat gggactatgt agaagataga tttggtaaga aggatttgct
     3181 tccttttaca atttcagata tggatttaga gtttccattt gaaataacag aggtgttgca
     3241 ggaaagatta aatcatagag ttttgggata cagtagatgg aaacatgatg attttagaag
     3301 ttcagttgcg aattggtaca ttaaaagatt taattgtagc gttgatagtg actggatata
     3361 ttatagtcca agtgtaatgt attcaattag caaacttatt gagatgttaa gtaatgaagg
     3421 agatggaatc cttataaata ctcctgcata taatgctttt tatgatgtga taacaaataa
     3481 caatcgtaat attataaagt cacatcttgt aaatagagat ggattctatt caattgattt
     3541 taatgatttt gagaagaagt gtaaaaaatc taagatattt attttatgta atcctcacaa
     3601 tccaactggt agagtatgga gtgagttaga acttgttaag atgataggaa tttgtaagga
     3661 taatgatgtt aagataatat cagatgatat acacatggat attgtatatg acaatagaat
     3721 gacacctatc ttaaatgttg caaaggacta tttagattct gtattcattt gtacatcagc
     3781 atctaaatct ttcaatatac cagcattgac tggatcatat gtgattatac caaatgaagg
     3841 tattagaaat aaatttgaaa atatcactag ataccgtgat tttgtaaatt caccagcaat
     3901 tctagcaata ttagctacaa tggtctgtta tgataactgc gaatattggt tagatgaact
     3961 attaaaatac cttaaagaga atcttaaata tacgattgat tatataaaag ataatttaga
     4021 accattgaaa ttggtcatgc cagaaggctg ttactttgct tggatagatt tttcccacct
     4081 taaaataagc agtgagaaat tccaaaaatt attgatggat attggagaag ttgctattat
     4141 gtcggggtct gtatatggtg aagaaggtga gttttattta aggttaaatg tggcatgttc
     4201 aagagaaaaa cttgaagatg gtttaaatag gattaaaaaa actatggatt atattgcaaa
     4261 aataacttta tagtaggaag ggagatagtt atgggatatt taaaaacaat ggttgatgtt
     4321 atgactactg aaatgaatgc cataagatgt ttaatagatg aagcaggaat agaatatgaa
     4381 agtatagtta gtgaaattgc aaattgtgaa ggcaaagtaa tttttatggg tgtaggaaaa
     4441 tctgcgcata taggtaagaa attggcagct acatttgcta gtactggtac accgagcttt
     4501 tttgtacatg caacagaggc agtacatgga gatttgggaa tgattgaaag taaggatatt
     4561 acaattttaa tatcaaatag tggaaattct atggaagtgg taaactgtat aaaatctatt
     4621 aaagcaatag gttctaagac aattgcattt acttcaaaca gaaattcagt attggcaaaa
     4681 gagtgtgatt atgctttaat atatcctgca aaggatgaag cagaccattt aaatctagca
     4741 ccaacaacat catctacaat tactttggtt ttaggagatt ctattgcatg tgcattatct
     4801 aagagtagta attttggttc ttcagatttt tataagtatc atccaggagg aagtttaggt
     4861 gagaagttaa aaacagctaa caatggataa aagcaaaaag gagagtagtt tttatgttca
     4921 aaatatttaa aaaaaaatca ataaaatcac ccatatcagg aaaagtaata gagttatcta
     4981 aaatacaaga tgttgtattt tctcaaaaaa ttatgggaga gggtgtagct atagattcta
     5041 caggagatat tgtatatgct ccaatcaatg gaaagatagc agtagtagca gagactaaac
     5101 atgcatttgt aatagaatta gaaaatggga tggaacttct tattcatgtt ggattagata
     5161 ctgtaaattt aaaaggcgaa ggttttgagg cattggtgag tataggagaa caagtgaaag
     5221 aaggaacacc aatgttgaaa attaaccgtc ctttgattga atcaaagggt atatcactta
     5281 ttactccagt tacaataact aatcattcag agtacaatat gaatacatgt aatgtaggca
     5341 atagtgttga aggtgggaaa gatactgtaa ttgagtttaa ataaagaact aaaatacaag
     5401 attaatattt ataaatactt ataaacttat gttttataag attaattgta tagatttgtt
     5461 gtataaaaaa gaactcttta attataaatt aaggagttct ttttattttg taaatattgt
     5521 ttagattt
//
LOCUS       DAEMAE010000226         5513 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEMAE010000226 DAEMAE010000000
VERSION     DAEMAE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.228"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..247
                     /locus_tag="KPH93_003204"
     CDS             23..247
                     /locus_tag="KPH93_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689977.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKENMIYVPQIEAYIDLNSIK"
     gene            274..798
                     /locus_tag="KPH93_003205"
     CDS             274..798
                     /locus_tag="KPH93_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1689978.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRVHSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLARVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(903..1049)
                     /locus_tag="KPH93_003206"
     CDS             complement(903..1049)
                     /locus_tag="KPH93_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689979.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1188..2114)
                     /locus_tag="KPH93_003207"
     CDS             complement(1188..2114)
                     /locus_tag="KPH93_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG1689980.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIALLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2304..4175
                     /locus_tag="KPH93_003208"
     CDS             2304..4175
                     /locus_tag="KPH93_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG1689981.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLDYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVMDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4267..5202)
                     /locus_tag="KPH93_003209"
     CDS             complement(4267..5202)
                     /locus_tag="KPH93_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG1689982.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         2208 a          590 c          883 g         1832 t
ORIGIN      
        1 tatataagga gtagataaaa atatgataat aaaacttact aaagacaatt ttaattttag
       61 atttaaacaa tatgaaggta tgttttttca tgctgaagta gttgaaggta tggagtccat
      121 tagatataaa ataactaaag ataaagaagg tacagaatta aaagaatttg gaagtacaca
      181 tataaaagag aatatgatat atgtacctca gatagaagca tacatagatt tgaacagtat
      241 aaaataaaga ttatgtttga gggggaatcc aatatgagta agcctataga tataacaata
      301 agacctataa gaagagaaga tgcacaagct tttaatgaaa ttagaagagt acacagtgtt
      361 atgagaaata ctttggcatt aatgagtgac agagtcgata aaactgaagc tatgttagct
      421 tctttgggag aaaatgatta tatgtttgta gctgaaacta ataataatgg agaaaaagat
      481 gtaataggct ttgctgggtt acatgtaaat tcttctccac gattgagaca tagtgcagaa
      541 ataggaataa gtgttgcaga gaattatcag ggaagaggag taggtaaaaa gcttatggaa
      601 caacttttag acatagcaga taattggatt atgttagcta gagtaggatt agaagtaatt
      661 gtggataatg aaaagggatt aaatttatat aaaaaactag gttttgaaat agagggaact
      721 aaaaaatatg ctgtaatcag agatggaaaa tttgaagatg tttatattat gggaagatat
      781 aataaaaatt taatttaaaa aagctaatag agaagtaaaa tagagtgtct ttaataaaaa
      841 atttacatta aagataatct attttacttc tctattttat taaaaaactt atttttaaat
      901 gtttacaaaa acattctata tacaaataaa attattagca atatcaacgc tattttaaaa
      961 gctaaactag caacagtttt aatgattttt acagaaacac ctatagcaac tatagcaaat
     1021 actattttaa aaagtaaatc catatccata tttaagcctc ctattctata atacatatag
     1081 atattattga caaatatcta ggaaaaaatt ccatcaatat actacaataa aatgtttata
     1141 tttgtaattt attgaatatg ttattatata atacataaaa aaataaatca agataagagt
     1201 aaaatactta taaaaggtat agatataact gaaaatagtg ttgtaaaaaa cacacattgt
     1261 gaagctagtg ttatattgga gtcatattga ttagccatta tagcagtatt tgcagcagca
     1321 ggcattgaag aaattactac tggtatagct aaaatcatct tatcatttat ccatcctttt
     1381 agaataaaat atacaatcac aggaagtacc aataatctta taaaagtgac tatatataat
     1441 tttttgttga caaaacaatc gagagctgaa gaattagcaa gtaaacttcc aataataatc
     1501 atggatattg gtgtagttat acttcccaac atctcaagtg gctcgtttat aaattggggt
     1561 aacctaagtc ctgttacaaa taaaaaaagt ccaataacaa tagctattgt agcagggctt
     1621 ataagcgatt ttattgaaaa acctctactt gtactgcctt tagataaaag ataaattcct
     1681 aaagtgaaag aaaataagtt aaaaggcaaa ttgaatattg cagtatagaa tattgcttca
     1741 tgacctaaaa ctgcttcaat tacaggataa cccataaaag caacatttga aaaaacaact
     1801 atatactgat atattcccaa atctttttta tcattacatt ttaaaatgta ttttaataaa
     1861 agtgcaatta taattgaaac tatatacata atcaaagatg taaataataa caatagtatt
     1921 ttttgtatca ttttactatc aaagttaatt tgcatggagc taattatcat tgatggtaaa
     1981 aaaactgtca ttgtcaaagt tgaaagctta gatgtgcagt ggtcatctaa taagttgaat
     2041 tttctgacaa aatatccaac taaaataatt ataaataaaa ctgcaacttg gataaaaata
     2101 ttgctgaaat tcataaagtg tcccccctat atatacatta tataccaatt acataataaa
     2161 atctaatgat tataaaattt aaaatttaat gttacaatat agtgacaaaa ggtaaaaatt
     2221 caatattata gcattaattg tggattataa tgtataataa tgtttgagag aatatatttt
     2281 ataaaatggg taggtagcga ataatgaaaa atacaaaatt ttctataaaa aaaataaaaa
     2341 atatatctaa tgacccgaaa gtcgtacata ctaagggtgc cctccttgaa aagatgggta
     2401 agatggaaag tgcaatagag ttgtataaga gtgctgcatt ggataattac gtaaaatctc
     2461 aatatacttt aggaaatata tatgaaagta aaaaacaatt taaagaagca gaaaagtggt
     2521 attcaatggc atataaaagt ggaagtgaag atgctgcttt tgatttaggt aatatgtact
     2581 ataagttaga tggctatgaa tatgctatat attggtatga aaagatagca aatttagatt
     2641 atttacaagc acaaaataat ttaggtgtat gccattttaa aatgaaagat tttgtaaggt
     2701 gtgaaaaatg gttaaaaaca gcagcagata agaatttagg taaagcttgt tttaatttag
     2761 gggtattata tacttttttg gagaaagatg atgaagcata cgaatactat aaaaaaggtt
     2821 ctgtgctttt agatgatgat gcaaaatata atttagcaat attaaatgga aaaaagaaag
     2881 ataataaatc aacagtaagt ttatataaac agttgtacaa gtcaggtcat atgaaaggtt
     2941 gctttaactt gggtatgatt atggaaataa atgataattt agaagatgca gagaagtatt
     3001 ataaaaagag tgctgataga gatgatgtga agtctgagta taggcttgca tatgtatatg
     3061 atagaaaaga agagctgggg gacgcaattt attactatga gaaagcaata gaaaaaaatc
     3121 atacactttc taagtataga cttgctaatt tatataatag agaaaatgag atagagaagg
     3181 caaaaactta ttataaaatg gctgctgaag atgatataac agaagcaaaa aataatctgg
     3241 caggtatata ttttgaagaa aaagattatg aaaatgctat taaatattat gaggatgcta
     3301 tagctgttgg atgcaaaagc tcccttgaaa atcttgggga tttatattat cagaatcaag
     3361 atatagaaaa agcgatttcg tattattcga ggattccaaa taatgtatct tgtcaaatca
     3421 aattaggaaa tatctatgaa gatttaaata atattgaaga agctataagt tggtataaga
     3481 aagcatctga aaatggagat acgaggtcta gttatagatt gggatgtata tatgaaagct
     3541 taggaaatac aaaaaatgct agaaagtatt ttgaaatggc aagtagcaaa aatcatatga
     3601 atgctcgtat acatctaggt agaatttatt ttagagaagg taaattggaa gagtctaaaa
     3661 tgatgtttga tacacctgcc aatgaaaata atgtgtatgc tcaacatatg gtaggattaa
     3721 tttatgatat gttctataaa gattatgtaa attctaaatt ttggtatgaa aaagctagag
     3781 cacaaggttg tgttgaatca atatataatt taggtcaaat ttatttaaaa ttaaatgatg
     3841 acgcagaagc tgagaaatat tataaagaag gtattaaata tggaagtaag aagtgtgagt
     3901 atatgcttgc aggtctttat tataaaaaga gcctagatat gtatatttct ttagcaaatg
     3961 aagagtacga taattgtgaa gagatagtag aaggttttcc taatttaaat atagactttg
     4021 atgaagtatt aattcctcca tttaagttgc aagaagaggt aatggatgaa gaagagtacg
     4081 taccaattta tatactaaat ataaaagaaa gtttagattc aatgctagaa gggcttgaaa
     4141 ctgaaatgat tatagatgaa gaacatgata gttaaaaagt attataagat tatatagatt
     4201 taaatacata aaagccatta ggataaatga aaataaatta attctaatgg cttttttatt
     4261 aatattttat aatagcttat ttcttgacat gaaaaatgta atcactatac atattataga
     4321 ggtaataaga agtattatcc aaaatccgtt tgggttatta gaaaatggca tgttgatgac
     4381 attcatacca aagaatcctg atattattgt aggtattgag aaaattagtg taactactgt
     4441 taaaatttgc ataacattat tttgattatt acttataatt gcactgaaag catccataat
     4501 tctactcaat atatctccgt atattttagc catatcaaga gcctgtctat tctcaactat
     4561 aacatcttct aataggtctt catcatctgg atatttcttt ataccagcag ttctaaccat
     4621 tttgtttaaa acaagttcat tagattttag tgaggtagag aagtatacca gagatttttc
     4681 taattccaat aattgaacca attctttgtt tttcattgat ttgtaaattt ctcgctctat
     4741 tatgattgtc attttattta tttttcttaa gtaatgcaag taataagctg catttttgtg
     4801 aagaatttga agcaaaaagc gagtcttttt aaacgtaaaa aaatctttaa tatgaccaac
     4861 tataaagtca tttaaaatct tagtttgagc atcacaaact gttacaatag cttcttcagt
     4921 tagtataata cctaagggta ttgtagtata atgagaagaa ttagagtcac tttcgtctac
     4981 tggtatatct ataagtataa gagtatgatt gtcttcaaca tctattcttg agcgttcttc
     5041 ttcatctagt gcagattcta tgctttctac atctatattt aataaattag aaatttcgtt
     5101 gatttcacta tgattaggtt caacaagatt tatccaacag ccatcttcaa aaaaactaag
     5161 tttctctaat tttgaatcta tagttttgta atatcttatc atggcaacac atcctttatt
     5221 cagttttata taaatctcat catttaatag tatctattaa ttaactaatt tatataactg
     5281 aatatatgta ttagttttga ttttagatat tattaagtga tgaaaatgta aaaaattctc
     5341 tccgcaacgg actagtatcc attgcccatc actctcctta tttattattt tatatatagc
     5401 tatttatgaa atgcgtattt taagcactct ttaatataat accttttcca ggtatataaa
     5461 tcaagttttg atttgattaa ataatatttt taccataatt tacacataaa gta
//
LOCUS       DAEMAE010000227         5471 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEMAE010000227 DAEMAE010000000
VERSION     DAEMAE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.229"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..556
                     /locus_tag="KPH93_003210"
     CDS             110..556
                     /locus_tag="KPH93_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1689983.1"
                     /translation="MESKFFNWYTQSLGGTLGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYILLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILTTVVFGF
                     IGARVYFIIPAILITFNLYSDILIAKLKCILCKNKENDANLKESEV"
     gene            826..1278
                     /gene="accB"
                     /locus_tag="KPH93_003211"
     CDS             826..1278
                     /gene="accB"
                     /locus_tag="KPH93_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG1689984.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            1294..2655
                     /gene="accC"
                     /locus_tag="KPH93_003212"
     CDS             1294..2655
                     /gene="accC"
                     /locus_tag="KPH93_003212"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG1689985.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEENNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            2670..3524
                     /locus_tag="KPH93_003213"
     CDS             2670..3524
                     /locus_tag="KPH93_003213"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG1689986.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            3545..4489
                     /locus_tag="KPH93_003214"
     CDS             3545..4489
                     /locus_tag="KPH93_003214"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG1689987.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSIVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYNKFRNIGKCL"
     gene            4678..4938
                     /locus_tag="KPH93_003215"
     CDS             4678..4938
                     /locus_tag="KPH93_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG1689988.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            5091..5462
                     /locus_tag="KPH93_003216"
     CDS             5091..5462
                     /locus_tag="KPH93_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG1689989.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILNCNGYEVE"
BASE COUNT         2110 a          591 c          984 g         1786 t
ORIGIN      
        1 ttaaacataa attaatgaaa ttaacaaaat tttattcata agcatataaa gatatatttt
       61 actatttaaa tgcatatcat tatatataca gttttaggag gtgaggtata tggaatctaa
      121 attttttaat tggtacactc aatccttagg aggaactctt ggcattatat catgtatatg
      181 cgcctactta aatggataca tgtttgttta tacaaatagt gctagcaaat actttgaaag
      241 tataggattt ctgggaataa tttcaagtta tatactactt ccactttgta tgcttacttt
      301 gatttttgga gttatatatt cttatactcc agaaacatcc gacaaaagta ttttcaatat
      361 accttttttt actttaaata aaagattaat acttactaca gtagtttttg gttttatagg
      421 tgctagagtt tattttataa ttcctgcaat tctaattact tttaacctat atagcgatat
      481 tttaatagct aaattaaagt gtatcctatg taagaacaaa gaaaatgatg ctaatttgaa
      541 agaaagtgaa gtctaaattg cataatattg gtatttgtct taaaaattcc cgaggtatta
      601 atttgtattc tcttgtattt tgcaaaaaat attgaatttt attcatttta tacttataat
      661 ttatattaat aatggaaaat taaaacaaat ataagttact ggttgttaat ttaaaattaa
      721 aagatatatt gatgtgttga ttctctagtc agcacttttt cttttataaa tttgtttttt
      781 gtgatataat attagcacca ggtaataata caagggggta acaaaatgaa catcaatgaa
      841 attaaggaat tattaaaggc catagactca actaacttag aatatgtaaa acttgaaagt
      901 agcgatttaa gattagaagt atctaaaaaa gctcaatcta caagttcacc tgttttaagt
      961 gtacaacaag aatcagtagt ggatctttct ttagaaaagc cagttgtaaa cgatacacct
     1021 gtcacatcta atgagaatct atcagtagta gtagctcctt tgatgggaac gttctatgat
     1081 tctccaagtc cagatgcaga tagctttgtt aaagttggcg atgtagttga agaaggcgac
     1141 actttatgta tattagaagc aatgaagctt atgaatgaga taacaagtga aattaaaggt
     1201 gaaataattg aagtattagt tagtaatgaa gaactagtag aatataatca acctttattt
     1261 aagataaaac cattgtagta ggaggtattt aagttgataa aaaaaatact agttgcaaat
     1321 agaggcgata tagcagttag aatcatcaga acatgtaaag agctaggtat aaaaactgtt
     1381 gctatctatt ctgaaataga taaagattgt tttcatagat atatagctga tgaatctatt
     1441 tgtataggac caaataatat aagtaagagt tataataata tagaaaatat aatctatctt
     1501 gctcttaaat taaagtgtga tgctattcat ccaggctttg gatttttatc agagaatcct
     1561 gaatttgcaa agcaatgtga agagaacaat attattttta taggtccaac aagagaacaa
     1621 atgattttga tgggagataa atctagagca agagaaacta tgatggaatt gaatatccct
     1681 gttgttcctg gttcagaatc tgttttgaaa actaaggaag aagctttaga agttgcaaga
     1741 gaaattggat atcctgttat gataaaagcc tcaagtggag gcggtggaaa aggtatgaga
     1801 atcgttagaa aagaagaaga acttttttca aattttgata tggcaagttc agaagctcta
     1861 gcagcatttt ctaatagtga tttatatatg gaaaagttta tagaaaatcc tagacatata
     1921 gaagtacaag tatttggaga taaacattca aatgcaatac atttaggtga tagagattgt
     1981 tctatgcaaa ggcgaaatca aaaagtaata gaagaatctt taagtccata cctttcagat
     2041 gaagaaagac aaaaacttca taaaattgca gtagatatag taaaaggtgt aggttatatc
     2101 ggtgcaggta ctattgagtt tatagttgac aaagataaaa acttttattt tatagagatg
     2161 aatacaagaa tacaagttga acatccagtt acagagatgg taacaaatct tgaccttata
     2221 aaacttcaaa tatctatagc taatggggat aaaattcctt ttaaacaaga agatattact
     2281 tttagaggtc atgctattga atgtagaata aatgcagaag attcatcaaa gaattttgct
     2341 ccatcaccag gtaagataga atcattaaat ttacctggag gttttggtgt taggtttgat
     2401 acttttgtat atgcaggata tactatacca cctttatatg attctatgat aggtaaatta
     2461 atatgttggg ctgaaactag agaagaatgt ataagtagaa tatatagagc tctagacgaa
     2521 attattgtag aaggtattaa tacaaatgta gaatttcaaa aggcacttgt aacaagtgag
     2581 gagtttagag aagatactca tcataccaaa tttatagaag atgtctttat gaagaaagaa
     2641 tttgctacat tataaggagt aaatagatta tgataaaaaa atttttatca tcaaaagaat
     2701 ctaagtatgt tacgatttct ttagatgaca actttaaaaa gaatagtgta gatgataaat
     2761 tttggaccta ttgcaaaggt tgtgatagtc atgtatttag aaaggacata gaagaaaatt
     2821 catttgtatg tccaaaatgt tcaagacatt atggcttaag agcaagaaaa agaataaatt
     2881 tattgattga taaaggaact tttatggagt ttaattctga tgtagaattt caaaatccac
     2941 taaattttcc taaatacaaa gaaaaagtag actcttataa ggaaaaaaca aaagagtcag
     3001 aagctgttgt cacaggttat ggaagaataa atggaattaa aacggttatt tgtgtaatga
     3061 atcctgattt tatgatggga agtatgggtt ctattgttgg ggaaaaaata acttattcaa
     3121 tagagtatgc tgctgaaaat aatttaccta taattatttg ttctgcttca ggaggagcta
     3181 gaatgcaaga aggtatggta tctcttatgc agatggcaaa gacttctcaa gcactttcta
     3241 aacttgaaga aaaatcattg ccatatatat cagttctaac tgaccctact actggaggag
     3301 taactgctag ttttgctatg cttggtgata ttataatctc tgagccaaat acgttgatag
     3361 gttttgcagg tccaagagta atagagcaaa caataaatca aaaacttcct gaaggatttc
     3421 aaacttctga gtttttactt gaaaaaggtt ttattgacat gatagtagat agaagaaaaa
     3481 tgaaggaagt attatatcaa attcttgcta tgcacaagaa atagggcata aaggaggaac
     3541 tgttttgatt gaaaataata gtgaaaaaat aaaagtctta gaaaatgata ttaaacaatt
     3601 aataactatt tctaaggcaa ataatataga tttaagtgat aaaataaatt cacttaatga
     3661 gaaattggaa aagcttaagg aagatgcttt ttcacatcta tcagcatatg aaaaggtaac
     3721 actaagtaga gatataaaga gacctacgac attagaatac atagagcata tttgttctaa
     3781 ttttttagaa cttcatggag ataggctttt taaagatgac ccttctatag ttggagggat
     3841 tggtcagata ggaaagttta atgttactat agttggacac caaaaaggaa gagatacaaa
     3901 agaaaatata aaaagaaatt ttggaatgcc tcatccagag ggatacagaa aagcattaag
     3961 acttatgaaa caagctgaaa aatttaatag acctataata acatttattg atacatcagg
     4021 agctttttgc ggtctagaag cagaggaaag aggacaagga gaagctattg caagaaactt
     4081 acttgaaatg agtaaacttt ctgttccagt aattactttt gtgattggcg aaggtggtag
     4141 tggaggagct ttaggtattg gtgtgggcaa tgatgtttgt atgttagaac attcagttta
     4201 ttctgtgatt tctccagaag ggctttctag tattctattt aaagattcat ctaaagctaa
     4261 agaggcttgt gatgttatga agcttacaag caatgattta tatgatttaa aaataataga
     4321 taagattata aaagaacctc ttggaggagc acaaaaagat gtagaagctg tttcaaaaga
     4381 aataaaagct tatattttag aaagattaaa tcattataaa gatatggata aagaagaaat
     4441 tatagctcaa agatataata aatttagaaa tattggaaaa tgcttataga atataaagaa
     4501 atttgtagtt ttttcataat aaaatggtta aaaaaaataa aggttttctt aaaactatta
     4561 tagaagtata tttttataaa agtatacttt aaatgatgat tgtaattagt aaatggcaat
     4621 tatgatttag tactttaggt aatggatact ttaacaaatt aacaaggaga tgttgaaatg
     4681 gaggtactaa aagtttcatc aaaatctaat cctaattctg tagctggagc cttggctgga
     4741 gtactaagag aacgaggcgt tgcagaaata caagctatag gagctggagc tctaaatcaa
     4801 gcgataaaat caattgcaat agcaagaggt tttgttgcac ctagtggaat ggatttagtt
     4861 tgcattcccg catttactga tatagaaatc gaaggtgata agaaaacagc cattaaactt
     4921 ataattgaac ctagataata tatctcttta ttagaattta attaagtttt aaaggttggc
     4981 tatagaaata ggatactata gtgaaccttt tttactttat atgttttatt ttatgtaatt
     5041 tttaatgtat aatcttaagt aaacaaataa gattggagga aatatacatt atgaaattaa
     5101 aattattggt agaagagtat gcagtatgta gattaaataa tgatagtaaa ataccaacat
     5161 ggatagatac agagaagttt tactctataa ctagaactga tgatgaatta agtgttgtat
     5221 gtttaaataa taatattcct tctgatgtta agtcagaaaa ggagtggaga atacttaaga
     5281 tattaggtcc tttagacttt tcacttgtag gaattttatc taagttaagc agcttgttag
     5341 cagacaacaa aataagcata tttgcaattt ctacatatga tacagactat atcttagtaa
     5401 aagaaaaaga tatagaaaat gcttgtaaaa ttttaaactg taatggatat gaagtagagt
     5461 agttaaatac t
//
LOCUS       DAEMAE010000228         5452 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEMAE010000228 DAEMAE010000000
VERSION     DAEMAE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.230"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(154..738)
                     /locus_tag="KPH93_003217"
     CDS             complement(154..738)
                     /locus_tag="KPH93_003217"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG1689990.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(823..1725)
                     /locus_tag="KPH93_003218"
     CDS             complement(823..1725)
                     /locus_tag="KPH93_003218"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG1689991.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDNELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIIGII"
     gene            complement(1718..2410)
                     /locus_tag="KPH93_003219"
     CDS             complement(1718..2410)
                     /locus_tag="KPH93_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG1689992.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTLMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNEGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(2449..3369)
                     /gene="pyrB"
                     /locus_tag="KPH93_003220"
     CDS             complement(2449..3369)
                     /gene="pyrB"
                     /locus_tag="KPH93_003220"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG1689993.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKNMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGISED"
     gene            complement(3666..4664)
                     /locus_tag="KPH93_003221"
     CDS             complement(3666..4664)
                     /locus_tag="KPH93_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1689994.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSINQNVDSTNTNQQNNNSNVPTANSNAVQAVLNLA
                     YSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTVSKANLQPG
                     DLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFVTAKRIL"
BASE COUNT         1672 a         1039 c          558 g         2183 t
ORIGIN      
        1 cttattttgt aaatatattt ttaacaaaaa aaacagctaa ccttcattta taaggctaac
       61 tgctttttat ttttaagtta tatgttatat tacaagtttt tactctaaaa aatttacctt
      121 ttttaagtaa cactatgaat aattaagttt ttattacatt cctaattcct tgaactctct
      181 acttccaggc ttaacaactg gtaattttcc ttccttacat aaaggacact catcagattc
      241 ataaacttga atatcaagtt ttatagcact atatataggc atacctatat catgattagt
      301 tctatctgct atacatgcaa cacctacaac ttctccacct aaggcttcta atactctttt
      361 tgtctccata gttgatttac cagtagttac aacgtcttca gcaattatta tctttgctcc
      421 ttttttaact tcaaatcctc ttcttaactc cattgtattg tcttttctct cagtaaatac
      481 agcttctttt cccaattgtc ttcctaactc ataagaaact attactccgc ccatagctgg
      541 tcctactact aagtctatat ctaagtcttt tatttgttca acaactgtac ttaatacctc
      601 agcagcatat tctgggaatc ttaatacttt tgcacattgt acatatctat tactgtgttt
      661 tcctgaagat aataaaaaat gtccctccaa caatgcatca cttttcttta atatatctac
      721 tacatctact ttactcatat tatttttcct ccatgttaat gttataaaat tattgtttat
      781 ttaatctata ttttttatct ttaaaatact atttattctg ccttatataa ttcctattat
      841 ttcgtctaaa ctttttattc cttctctatc cataaattca ttaattccat ctattatttc
      901 aatacctatt ctaggattca taaagtttgc agttccaact tggatacaag tagctcctgc
      961 cattataaat tctatagcat ctgtagcttt agttattcct cccattccca ttacaggtat
     1021 atttacattt ttacacactt catgtaccat tcttaaagct ataggtttta tagctggtcc
     1081 agataaccca gcatatacat tttcaaatac tggtcttcta ttttttatat caattgccat
     1141 tgctttaaaa gtattaacta atgaaactcc atcagcgcct tcttcttcac aaacttttgc
     1201 cattcctaca atgtcttctg cattaggtga taattttatt actaaaggca gtttcgttat
     1261 gtttcttacc tcacgaacaa cttctcttgc tacttcattt tttataccaa aagccattcc
     1321 accagctttg acatttgggc aagatatatt taattcaata atgtctatag gtttatcatt
     1381 taacatttga acccctaata agtagtcttc taatgtccca cctccaacat tagctattct
     1441 gactaagtct agtttactaa aaaaaggtag ttcattatct ataaatcctt gaactccagg
     1501 gttttcaagc ccaacactat tcatcattcc agatggtgtc tcatgaactc tcataccatt
     1561 gttaccaggt tttttattta aagtaagacc tttgctactt ataccaccaa gtttttgtat
     1621 atcataaatc tcattatatt cttttccaaa tccaaaagtt ccagacgcca ttattactgg
     1681 atttttaaat tctacacttc caaatttcac acttaaatta cccattaaaa atcacgtcct
     1741 ctccccagaa cactggacca tctttacaag tctttttatt tccttcatta gttttacacg
     1801 tacacactaa acaggcacct actccacaag ccatatggtt ttcaagagat accataattc
     1861 tcgttttagt accttctacc atcttaacta atttttccat cattagagtt ggaccacatg
     1921 ttaatatata atcatatttc tcaacatcaa ttatatctgt tacaaaagta tttcctactg
     1981 ttgtatagac tttattacat acttgcttat attcatcttc taatataaca tcttctctaa
     2041 acccaagata tgcgtcacaa ttttttatat ttttagcaac caaatatagt ggagctacac
     2101 cgattcctcc tcctactaga gctacctttc catcaacctt agcatatcca ttaccataag
     2161 ggccttctaa ttttatagta tcatttacct ttaggttact caaaatttga gttccttctc
     2221 ctactacttt ataaagaaaa gttatccctt cttcatctat atcatgtata cttataggtc
     2281 ttgaaagtac tggataagta tcccatgctc ttagcatata gaattgtccc attttacctt
     2341 caaaattacc ttttactttc attctataca tatcttcgcc tatatatata ttttccagaa
     2401 ttttatacat cccaaaatct cctttgtttt actattatat gataactatt agtcttcact
     2461 tatacctaaa agttttatta ttaaagccat tctaacatac attccatatt tagcttgttt
     2521 aaaatataca gctctatcat cactatctac atctatatct atttcattta ctcttggtaa
     2581 tggatgcatt actaacatat ttttagatgc cttttctaat ttagctttat ttaaaatata
     2641 atagttttta agtctttcat actctgattt atcttcaaat ctttcttgtt gaactctagt
     2701 catatataaa acatccaagc ttcctataac atcatccaaa ttattagttt cataatatgc
     2761 atgtcccttt atagcttctt ttatataatc tggcattttt aactcttcag gcgctataaa
     2821 tacaaacttt gtatttttat atcttgccat agccttaacc aaagaatgca cagttcttcc
     2881 atattttaaa tcaccacata atcctattgt atggctttct aaagttcctt ttaatgattt
     2941 tatagtaagt aagtctgtaa gtgtttgagt tggatgctgg tttttgccat ctcctgcatt
     3001 tataaatggc acttccgtat atctagctgc ttctgaagct gcacctgatt gaggatgtct
     3061 catagctatt atatcaacat aaccagatac tatcctcatt gtatctccta gagtttcacc
     3121 ttttgatgca gaggatgaat ttggctctga aaagcctact acacgtcctc ctaatctgtt
     3181 catagcagat tcaaagctta atcttgttct tgttgatggt tcataaaata atgtagctaa
     3241 tagttttcct tcacatatac gagaatattt tgacggattg tcaattatct tttgagctaa
     3301 ttctaatatt tcatctattt cttctattga aaagtcttct ggttggatta aatttcttga
     3361 ttttaacatc tttcgtcctc cttttttagc ctctctgaac taaaattaaa agtatttttg
     3421 tactgttagt agcttaccac ctttacccta tcctgtcaag ggattgcaca aataatcatc
     3481 atatgaaaag gctcctattt aacagtcata ttaacttgaa tcttgattag atttaaagtg
     3541 atacaaaaac gctctatagt acatttaaaa tataatcatt attttgtttc catgattaaa
     3601 ttaaatataa tatagagcgt ttatatatat ttatgtattt atcaatttgt catattacaa
     3661 accgattata atattctttt tgctgtaaca aatcttgctg tatagtatga tgaatttata
     3721 ctagttacac taactgtttt tcctgttgat ggtgagtgta tcatattacc cccacctaca
     3781 tatattccaa catgactaac agaaccagaa ccattagttg tgaagaatac taagtctcct
     3841 ggttgtaaat ttgctttact tacagtttgt ccatatttag attgtgcttt agatgttctt
     3901 ggtatattct ttcctactgc atttctatac acatattgtg taaatccaga acagtcaaaa
     3961 gtgtttggtc cttctgctcc ccaaacatat gggcaacctt gcttagaata tgccaagtta
     4021 agtacagctt gaactgcatt agagttagct gttggcacat ttgaattatt attttgttga
     4081 ttagtatttg tagaatctac attttggtta atacttgggt tcacttgtgg ctcttctatt
     4141 acaggagctt tatcagatat ataagatgat tttacatatc cttctgtttc cccatcttta
     4201 acttttaacc aatctccatt atcttccaaa accaataaag aacttccttt ttccaactct
     4261 tcaattactt ctgaactttc actcttatct tttcttaagt ttactttatc agcgttagta
     4321 tatcctacac cttcagatat atctacatat ctagtagcta accaaccttc accatcttca
     4381 actttaacct taacccattc tccttgagtt cctaaaacag taaattcatc tccagaataa
     4441 gctatatttt gaacttgacc ttcttctctt atctttatag ctactccatc ttttattttt
     4501 gcaactttat attttacttc tttatattca cctaaattta attcagtatt tgttttatca
     4561 tccttatttt gagatgaatc atttacttca tctgcattta caattgaact tactgataat
     4621 gccataactg atgcaaatac tggtattaat atctttttct tcacgataaa cctcctaaaa
     4681 ttttcccata attatcttat gattctattt tgtaggtgaa gtgtacctaa ggataaaata
     4741 ctaatacaca atacattaat aaaagctcta tctgtaactt ttttgtaaac cactttatat
     4801 aacttttata gtatcattct attaataaaa taaataaatt agcttatcca atatgtcacc
     4861 taaatataca ttatttactt aattatataa tatgggtcta taaaagtaaa gaattttgta
     4921 ccattcaagg ttaaagtgcg cataaatatt gttttatgtg tttttttatg cacttttata
     4981 tgtcgtatat tttttgatgg atttagtata aagttagata ttctgtgaaa tttttaaccc
     5041 tacataaatc aaagcctatt tcctaatagt gattctcaaa ttctaataat tctaagttaa
     5101 aaagatgaaa attttggaga atataaatat tcaaaaatta agtatgaaaa gattgtccta
     5161 gcagtaatct tttcatactt aattttttta gataatatat caatagtaac tatcattaag
     5221 atataatagt taaatttttt actaactttt ttaaatacat tattatcaaa gattaatata
     5281 agaagaatgc ttaaactact tagtataaca actttgaaaa ttccatttta aatgaagttc
     5341 gacatacact tttcccaatt gtattttaca cctgaattta tctatttttt ttaaattata
     5401 aaccttacaa ttaataattt atttcactta cttaagttct ttataagtat at
//
LOCUS       DAEMAE010000229         5450 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEMAE010000229 DAEMAE010000000
VERSION     DAEMAE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.231"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(92..1159)
                     /locus_tag="KPH93_003222"
     CDS             complement(92..1159)
                     /locus_tag="KPH93_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1689995.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            complement(1171..2859)
                     /locus_tag="KPH93_003223"
     CDS             complement(1171..2859)
                     /locus_tag="KPH93_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG1689996.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYIENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     NFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVVGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYFLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            complement(2877..3872)
                     /locus_tag="KPH93_003224"
     CDS             complement(2877..3872)
                     /locus_tag="KPH93_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1689997.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            complement(3873..4709)
                     /locus_tag="KPH93_003225"
     CDS             complement(3873..4709)
                     /locus_tag="KPH93_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG1689998.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGVTIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLFYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            complement(4713..>5450)
                     /locus_tag="KPH93_003226"
     CDS             complement(4713..>5450)
                     /locus_tag="KPH93_003226"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG1689999.1"
                     /translation="RYEVLYYEGLDKVLELLDENKIDYLSIMDHSPGQGQYTNPTFYR
                     EYATKVWGVTENYVDTWLDDLVNLHDNLDWNKIANIIGIAKTKNINVASHDDDTLEKL
                     DFIKNIGINVSEFPITLEVAKHSKKLGVLTCLGAPNIVRGKSHNNNLKAMDAILEDCC
                     DIVCSDYLPSAMIKSMCIVAERINNLNKAVSLFTSNPAKAVGIYDERGSIKENKKADL
                     VLVDIDSEYPKVVNTIVNGKTVYKREV"
BASE COUNT         1707 a          972 c          644 g         2127 t
ORIGIN      
        1 tttcttgacc tatattattc tcaacttata ttattataat aaaatctact taaaaaaatt
       61 ctttcttttt actccatttg tatagtttta cttaactctc tttttctgtc ttaccatcat
      121 attttgatat ccatttattt agtatatctt ctctattttt tgctgccgat tttaaatcat
      181 tatctatcat ttgctctaat ggctttttag ggaatccttc tggtattgga tttcctgtag
      241 aaatagtagt aactgcatag ttcttagagt attcgttcat tgcatcttta cttatagccc
      301 aatctaaaaa tacttttgat gcttctttta tattatcttt tttcacaaga gcatttgctt
      361 ctatatccca tccagaacct tcttttggaa atactacttc tactggatag ccttcctctt
      421 ttaattttat acctctatat ccaaaagata taccaatagg atattctcct gaagctgctg
      481 atgtagctgg agctgaacca gattgagtgt atacaccaat attctcatgc aacttatcca
      541 tatactgcca tgctttttct tctcccataa tttgtataag ggctgatact gttaagtacc
      601 ctgtaccaga agaagatgga ttaggcatag atataagacc tttatattct ggctttatta
      661 aatcttcgta agaactagga actggtatgt tcttatcact taattcttta gtgtttactg
      721 ttatacctgt catccaagca tctatcccaa cccaagaagg aacttcttta tcatctttaa
      781 atgcagactc tatttcttca gaaccttttg gagcatatgg ttctagcatg ccctggtcat
      841 ctgcgaccaa taagcttgtg gctgcaactc cccatacaac atctgcttga ggattatcct
      901 tttcagctaa taatcttgct gttatatctc ctgttgatgc cctaacaata tttaatttaa
      961 tatctggata tttctcttta aatgtagcta aatatggctc tatactatct tcttcaattg
     1021 ctgtgtaaac tgttaaatcc ccttttgtct tactatcaga ctttgaatct gaacctgatg
     1081 aatcagtact acatcctgtt attacactta ataaaagtgt tgataatact cctaaagaaa
     1141 ctagcttctt aaatttcatt tttacctccc ctatgcaaca tctttttgct gccatttttg
     1201 tgtgttattt tttaatttct ttgttacaaa ttcgtacaaa agtcttacta ttatatttgt
     1261 taaaagcact aatactgcca tagcagctga tggtcctata taccctatct catctaactt
     1321 tagtattgat attgatgcag tctgagtttg aggtgtatat aaaaatatta atgctgaaac
     1381 agtaaccatt gaatttacaa aataatatac tactatttca agtattgatg ttatagacat
     1441 tggaactgtt atattaaaaa atactttata gaatggaata tttatggatt ttgcaactaa
     1501 ttcatactct ctatccaaca tttttaaaga tgaatttgaa gttataaaac aaactgaata
     1561 aaaatgaact atatttacta taactaatat tgctgtactt ccatataaaa aatttaatgg
     1621 attacttgct gaattgaaaa aagttatata tgatatacct aaaactaagc caggtaaggc
     1681 cattggtatc attgataaaa agtaagctgt ttgtcttaat ttaggaaact tatccacttt
     1741 ttctatcata taagcactaa aaaatacaaa tatagttcca aatattgctg ttaaagttga
     1801 tattttcaaa ctgcttaagt atgtttttat tccacttccc aaaatacctt taaatgtata
     1861 gtgttccatt gtaaagctta aattatatgg ccataacttt ataactgaac ctacaactga
     1921 aacgaatatt aaaataaaaa ttagcaatgt tataaaacta catactatta aaaatactct
     1981 atctctaaac ttattttctt taatcttata tggcatagac tttgcagtta tggtcatacc
     2041 ttgtttctta cttgccatct tatctaatgt aaatgctatt agtacaggaa ttaacattac
     2101 catagagaca gtagctccca ttggtatgtt aaattgtcca actacttgtt tatatatgtc
     2161 agtggccagt acattgtagt ttcctccgac taccttagct gctccaaaat ctgtaaatgc
     2221 aagtataaag gatacaaaga atgaactaag taacgagtac tttataccag gaattgttat
     2281 tttgcaaaat ttcttaaatt cactagcgcc caagctatct gctgcttcat aaagtctgta
     2341 atcagccatc gaaagtgcta cagataatat taaaaaggcc tgtggaaatg taaatagaat
     2401 ctgagaaata attattccta atggaccata tagtggtact tccaaaccca ttgttgtaaa
     2461 gattccctta tttccaaaaa ggtatataag tgaaatacca tatagcatag ttggtgcaaa
     2521 aagtggaaac attcccatag ttttaaaaaa atttttaaac tttatgtttg ttcttgttat
     2581 tgcgtatgaa tatataaatg ctaaacatag tgatattaaa gatgagactg tagatacaaa
     2641 aattgaattt ttaaatgatg ctataagact tggattttca atatatttac taaagtttga
     2701 aattcctata aaatttccac ttgtatccat aaatgcattt tttaataatg atactaatgg
     2761 aaataatata aatactatta gtacaattac cgaaaaaatc aagaaatatt tttgtattgt
     2821 gctatcactt attcttcttt tattttttga tgttatcatt tattcacccc tagctattaa
     2881 gcacatccct ctgatttata ttgtacaatt ccttctttag gtatcttaaa gtttatttta
     2941 gaacctatgc acagtttcat ttgttctgca actttggaaa ctacatctaa tttcatttgc
     3001 ttagaacgat actcaactgt aattctatag ttaaatcctc taaattcgat atctaaaatt
     3061 tctcccactt ttatatcctt atctttagaa tctctattgg actctacttt tatatcttct
     3121 ggtctaatag ctatcttatt aataccatca tctataaaat ttattgttcc tataaaatca
     3181 gctacaaaag gtgtttctgg atttttatat acctcctttg gcgttccaac ttgtattatt
     3241 tctcctctat tcataacaac catcttatca gctaaacaaa gagcttcttc ttggtcatgt
     3301 gttaccatta tagttgtaat gcctagtttt ctatgtaaac ttctaatctg ctccctaagt
     3361 ttaagtctta cttttgcatc taatgcagat aaaggttcat caagaagtaa aaagtctggc
     3421 tctaaagcaa tcgcacgagc taatgcaacc ctttgttgtt gtccacctga taattgtgat
     3481 ggatattttt tctcatatcc actcaattct acaacgtcta gaacttcttt aactttttta
     3541 tcaatattag atttacttat gtttttattt tctagtccaa aagctatgtt tttgtaaaca
     3601 ttcatatttg gaaataatgc atatgattga aaaactatac caaaatttct actttcaggt
     3661 ggtgaattag tcatatcttt atcatttatt attatttttc caccatgttt atcctcaagc
     3721 cctgcaatta ttctaagcag tgtacttttt ccacaaccac ttggacctaa taagcaaata
     3781 aactcccctt tttcaatatc aatagatatg tcttttaaca ccttaacttg tccataactt
     3841 ttacctacat tttttacttg taaaaaagac atttataata cccctcctaa agctaaccta
     3901 aacttaaata agtcttcttt atttaagctc ttgcttccaa gttcataatt atgttgagaa
     3961 taaaatccgt ggtaatctga gcctcctgtg accactagat tgtacttctt agcataaaaa
     4021 agcgattctt tttctagttc tttgttgtga gatggatgat atacttctat tccatctaat
     4081 cctactttta ccatctcttc aatagcgcta aaattatcca tttgacctgg gtgagctaac
     4141 acacaaattc caccagaatt ttttatagct tttattgcat ctctgtaatc aacatatttt
     4201 aaagatacat ataccttgcc gtttcctcta tgaaacaatt ctttatatag acttgaatat
     4261 atattatctg tatatccttt atctataaga gccttcatta tatgttgttt aaatattcca
     4321 gtctttgatg agtatttctt tacatcctcc agagatattt tgtaccctaa atctataatt
     4381 ttatttacca tcttaacaga tgctttatgt ctgtcacata ccattggatt gcacaaattt
     4441 tttatttctt gactgtttaa atctatgtta tatccaagaa tatgaactct ttttctattt
     4501 ttataatcat aagctgaaat ttctatacct ggtattattg ttactccata atgttctcca
     4561 agcacaagag cttcatccaa tccagataat gtgtcatgat ttgttattgc taagatattt
     4621 atattctttt ctgctgcttt ttttattatt tcttcagtag taaaattatt atctgatata
     4681 cttgtatgac agtgtaactc tgttaacaat ctttatacct cccttttata aactgttttt
     4741 ccgtttacaa tagtatttac aacctttgga tattcactat caatatctac taaaactaaa
     4801 tctgcttttt tattttcttt tatactacct ctttcatcat atataccaac tgctttagca
     4861 gggtttgagg taaataaact gactgcttta tttaaattat ttattctttc tgcaactata
     4921 cacatagact ttatcatagc tgatggaaga taatctgaac acacaatgtc acaacaatct
     4981 tccaatatag catccattgc ttttaaattg ttattatgag atttacctct tacaatattc
     5041 ggtgcaccta aacaagttag aacacccaat ttctttgaat gttttgcaac ttcaagagtt
     5101 ataggaaatt cagatacatt tatgcctata ttttttatga aatccagttt ttctaatgta
     5161 tcatcatcat gtgatgcaac atttatattt ttagttttgg ctatacctat aatattagct
     5221 attttattcc aatctaaatt atcatgcaga tttactaaat catctaacca tgtatctaca
     5281 taattttctg taactcccca cactttagta gcatactctc tataaaatgt tggatttgta
     5341 tactgacctt gtcccggaga atggtccatt attgataagt aatctatttt attttcatct
     5401 aatagttcta aaaccttatc taaaccttca taatacaaca cttcatatct
//
LOCUS       DAEMAE010000230         5366 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEMAE010000230 DAEMAE010000000
VERSION     DAEMAE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.232"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..665
                     /locus_tag="KPH93_003227"
     CDS             168..665
                     /locus_tag="KPH93_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG1690000.1"
                     /translation="MYKIGESVMYPKEGACCVNDIVTKKINHQMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDKFIQSIDKTDGVWVFDRKQRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDCSKQLCSTDKEFLNKAQRFALSELAAAQCKSYTVVLEEMKKHILN
                     SKSKN"
     gene            complement(838..1086)
                     /locus_tag="KPH93_003228"
     CDS             complement(838..1086)
                     /locus_tag="KPH93_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690001.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(1152..2168)
                     /locus_tag="KPH93_003229"
     CDS             complement(1152..2168)
                     /locus_tag="KPH93_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG1690002.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKIKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVEQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSGSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(2226..2993)
                     /locus_tag="KPH93_003230"
     CDS             complement(2226..2993)
                     /locus_tag="KPH93_003230"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG1690003.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRIDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENDGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDITQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(3061..5250)
                     /locus_tag="KPH93_003231"
     CDS             complement(3061..5250)
                     /locus_tag="KPH93_003231"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG1690004.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRDLAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKENKKENSKKEYYKNNKKEKDN
                     KDKKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         1641 a         1028 c          537 g         2160 t
ORIGIN      
        1 tatatttttc tatttagttt tatataccat ctaatctaaa cttaatatta tatattaata
       61 tttttgactt gtcaagtttt ttatggtata atattaatta ttagataatt aaaacactgt
      121 acctaagttg caatatctaa aattcagttt ggaggtataa ctaatatatg tataaaattg
      181 gtgaaagtgt gatgtatcca aaagagggag cctgctgtgt aaatgatatt gtaacaaaga
      241 aaataaatca tcagatgcaa aaatactatg aattaagtgt cattttcaat agtaacctta
      301 aaatttcgat tccagttcta aatgcagaca ggattggcat acgtccagtt atggatggaa
      361 atgacgttga taaatttatt caatctattg ataaaacaga tggtgtatgg gtttttgata
      421 gaaaacaaag gcttaaactg taccatgata aatttcattc aggggatgtt tttgagattg
      481 taaaattaat taaaatgctt atgatacaag actgttcaaa acaattgtgt agtacagata
      541 aggagttttt aaataaagct caaagatttg ctttatcaga attggctgct gctcaatgta
      601 aatcttatac agtagtatta gaagaaatga aaaaacatat actaaattct aaaagtaaaa
      661 attaaaccag tcttaaagac tggttttatt tttatactaa tcatttatgc tactctatct
      721 tactttaaac tgcctaatct aatattaata aagttacccc aattagatag atgtctttat
      781 tcaaataaaa aaactaagag taaatcatat tactggttta ctcttagcta ataaaactta
      841 atcttcttca agttcttttt tctcttgtaa taatacactt aattcatttg cttctaaaac
      901 ttgtgcctct gcaatatcaa gtaactcttg ttgccattcc acacatttat ctttgtaatc
      961 aaaaggtagc gttctgtcaa ctatgtactt atcaatacag tcctccgcac ttttaattat
     1021 ttcttttctt tcatcaataa atttttctaa ttctactaag gcttcttcat aagcttttct
     1081 actcatataa attccccctt tgtacaatta ttctacatta aaatgatttt tcctcttttt
     1141 tgtataaaat tttatttgta tataactttt ccatcaatat cttttatagt ttgacatcca
     1201 gttaatatca tagagccact tagctctgat tgaagtttat ttacataagt ttctacacca
     1261 tcagttgcac caccaaatga agcagtcaca aaaggtcttc ctattaatac tgcatctgca
     1321 cctaaaccta tcatttttag tacatctaaa ccagttctca ctccaccatc tgcaagtatt
     1381 gtaactttac ctttaacagc atcagcaatt tttggtaaaa cttcacatgc tcctggcgta
     1441 cagtcaagta ctcttcctcc atgatttgat acaactattg catccacacc tgcttctact
     1501 gccattaaag catcctcaac agtcattata cctttcaata taaatggtag tttagtagac
     1561 tttactaact cctttatttg ttcaacattt ttaggaagta ctggttttcc atgtaatgat
     1621 aaagttacta gaccacaggc gtctatatca actccaactg caaatgcacc agcttcttca
     1681 gctaatctta tcttttttat aatattttca ttatcccaag gtttaataaa tacaattcca
     1741 gctccgttat actttttgac tacatctaaa ttttccatta aaaaggcatc tactgccgta
     1801 tcaccaacca ttgcataaat accactatta gcacatcctg caactactgg ttctatgtaa
     1861 tctctttcat taatttttcc acccatattt aaagtagttc ccgttactgg agctgcaaat
     1921 ataggtgcac tcattttctt tccaaacatc tctatagatg tatcaggatt cgatacatca
     1981 tgtattactc tcatatttac ttttattttt tctaaactct tgctattttc tataaaagaa
     2041 gaaccacttc ctttacctcc catgccaggc acttcacctg cacaagcaac cccattacaa
     2101 accttacaaa ctttacaact tccatttaaa ttttctctag cactttttaa taattcatta
     2161 tattccatct ttaatccccc attaactata ttgatttata ttatatacat tatatcatat
     2221 tatatttaca gtatattttt taaaaaactt tttctgtgga taggagttat gccatatttt
     2281 tcaatagctt catagtgttc tttagttcca tatcctttgt gagatttaaa cccatattct
     2341 gggtatactc tatcatattg gtacattata ctatccctag taactttagc aagtatactt
     2401 gctgctgcta ttgatataga ttttgaatct ccttttacaa ttggattttg agttatatca
     2461 atacctggta ttgttgctgc atcaactaac aaataatctg gtgctttttt taaacaattt
     2521 atagcctttt tcattgccat atatgtagca tttaatatat taaattcatc tatttcttca
     2581 ttgtttacta tacctattcc ataatctagt gcttcttctt ttattacttc aaaaagttca
     2641 tctctttttg cctcactcag tttcttagag tcatttacac cttctatttt tgtatctttt
     2701 ttaaatacta caacagaagc tacaacaggt ccagcaagtg gccctcttcc cgcctcatca
     2761 attcctccaa tatataaata acctttatca tagccatcgt tctcaaatat atttatagtt
     2821 tccaatctct cttcttcttt tctaatatta tccagctttt ttgctagttt tatagctaaa
     2881 ccttgtacag attttctttc atcaatcctt aacagttcta tatattccat atatttttct
     2941 acttctaaag tctctattat ttctttaatc tccctcacac ttttatcctg cataccattc
     3001 cctcatcttt cgtttttaaa aatttaagtt ttaaatatat taaagttttt taattagagt
     3061 ttaattgcat ccactatagc cacattttaa acatatacta catccacctg tatgtgcaat
     3121 tccttcactt ccacactcag ggcacacaaa tttttcatct tcttcaaaat ttttatttat
     3181 tatttctttg gcttttttat ctttattatc tttttctttt ttattatttt tataatattc
     3241 ttttttacta ttttcttttt tattttcttt attattttct tccttttttt catttattaa
     3301 aattccactc cttttacatc catctctaaa tattgttact cctttaagac cagcctccca
     3361 tgccttaata tatatattga aaacatcttc aactgtagtt tcaaaaggta tatttattgt
     3421 agatgatata gaagcatcaa tatacttttg ccatactttt tgcatgttta ttctttcttc
     3481 atatcctata ttcatagcag taacaaaaaa atctggtaaa cattcttcat ctgtaatacc
     3541 tttcttatcc atatactctt taacaattgg agtatatact ttataataca catcttcatc
     3601 atgcaagctt tcagtttttc ttatatacga aagattaaat ataggttcta ttccaccact
     3661 aattcctatc attgtagata tagaacctgt aggaggtata gttaaaagtt gtgaatttct
     3721 aagcccatac tttttaacca tttccttagt gtcttcattt agattttcta taaaaaactt
     3781 agatttagaa atattttcat atttatactc actaaatgtt ccatattctt tagcaattaa
     3841 tgctgattgc ttcacagctt catttaacat agtcttacca attaaatcac atagttctaa
     3901 agattcatca gaaccatatc ttattcccat ttttataagc atatcagcta tacccattac
     3961 acctaaacct atctgtctat acttatgtac actatctctt tgttcttgca gtgggtgcaa
     4021 gttcaatccc tcatctaaga catcatttaa agctactaca gcttctctaa cgcaagattt
     4081 taatttatta atatcaaaag tagcatgttc accaaatggt tgtattataa actctgacaa
     4141 gtttattgag ccaagtagac aacttcctcc acttggaagt ggctcttctg cacaaggatt
     4201 tactcctgaa aagcaaaagt tcttatcttc actcataaaa ttatatgttt ttattttatc
     4261 ccaaaacaat atacctggtt ctgcataatc ccagttattt ttcacaaatt tattaaataa
     4321 atcataagca ttcactatct taactatttt ttctccagta gtttcaactt caaaagtaca
     4381 attatataac tttttttctc ttacagcttg cataaactca tctgttactc ttacagaaat
     4441 atttgcttta gtaaccttct ccaaatctgt ttttatatct ataaactctt ctatctctgg
     4501 atgatttaca tccatagaaa tcataagagc tcctcttctc cctctttgac caattaagcc
     4561 cgttgtcata ctgtaaagtt ccataaaaga gactgctcca gtcgtatgtt ttgcagcatt
     4621 gttaactttc gctccttttg gtcttagctt tgaaatatca attccaactc ctccaccata
     4681 actaaaagtt ctagcaagtt tttttgcagt atcaaatata tcttctatgc tatcttttgg
     4741 tggttctatt acataacaat tggaatatgt tacttttttt ccttctttct gtaatcctct
     4801 atttgctaaa attcttccag caaatagaaa tcttttttgt cttattagtt ttgatattct
     4861 tgaactatta tttgaaattc tctttatcca ttcatcaaaa ctctcaccat tatgtctata
     4921 cttgttcttc catattgatt tttgtaattc atcaaactcc caagctttag tccttatttc
     4981 agagcgtctt tccctatata atatgtagtg ttttgctata tcttttctat ttgattccat
     5041 aagtagtact tcaactatat cttgtatttc ttctacggat aagcattttt ccatagaatt
     5101 atagtcatta gctatttttc ttgatatatc atatgccaaa tctctatcta ctccaccttc
     5161 actatttata ctagctttat agacagcatc ttcaatctta cttttactaa aaagttcttt
     5221 tgtaccatct ctttttatta tgtacttcat ttttagacct ccaataaatt agtattaaat
     5281 taatacacac attaactata actctaaaca aactttttat atatttgtat attttcatat
     5341 taattatcta acataatata ttatac
//
LOCUS       DAEMAE010000231         5354 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEMAE010000231 DAEMAE010000000
VERSION     DAEMAE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.233"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(206..1390)
                     /locus_tag="KPH93_003232"
     CDS             complement(206..1390)
                     /locus_tag="KPH93_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1690005.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMENNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYIGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIENVGYVNPNGAFYTFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1763..4114
                     /locus_tag="KPH93_003233"
     CDS             1763..4114
                     /locus_tag="KPH93_003233"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG1690006.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDEELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRYKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4136..4675
                     /gene="nrdG"
                     /locus_tag="KPH93_003234"
     CDS             4136..4675
                     /gene="nrdG"
                     /locus_tag="KPH93_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG1690007.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFVTEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2092 a          597 c          849 g         1816 t
ORIGIN      
        1 tataataaat ttagaattta acttatatag tttaatttga taatttgatg aaaaaaataa
       61 aagacttact gatttttaaa tctataagtc ttttattttt tatctaacat ttaaattaag
      121 ttgttgaata tatcttatat tacttacttt atatattaat tatcattaat acattatatc
      181 taattactat tcatctaaat tgttattatg ccattatttt atatacaaat tcttttagtt
      241 tatctaatcc agataagaaa gtattttcac tgcaagcata agatatacga atatatttat
      301 ccataccaaa tgctatgcca ggaacaactg caacattata ttcttctaaa aattgattac
      361 aaaattctat agaaaaacta tctttatatt caaatttctc agatacttta gaaagatcta
      421 taaatgtata gaatgcgcca tttggattca catatcctac attctcaata gagtctagtt
      481 ttgatttaat taaatctctt cttgatttat atgttttaac catattgtct atgtcaattg
      541 agcaatcttt caaggctcca taggctatgt attgtgctgt taaacttggg tgagatataa
      601 gatgtccttg tatagaactc attgctttag caatcgtttt attagatgca gtatatccta
      661 atcttaatcc agtcattgca gccgacttag aaaaaccatt tatagttatt gttatgtctt
      721 tagcttcttc gcttaatgat gcaattgatg taaattcacc aatgtagcat attttttcat
      781 atatttcatc tgctaaaata tatattttat tttgtatgca tacatctact atttctatta
      841 attcatcttt tgtataaaca ctaccagtag gatttgaagg attgtttatt acaagaattt
      901 ttgttttatc tgttatagat ttttcaagtt cttcttttgt taacttaaat ccattttctt
      961 ttttagtatc aataaaaact ggaacagcat taactaactt aaccatttca ggataactaa
     1021 cccaataagg ctttggtaat aagacttcat ctccttcgtc tgttaaagct agtaaagtat
     1081 ttgttattga gttttttgct ccacttgata caactatttc gtctattgaa tagttacaat
     1141 tattttcttc aataagtttt ttacatattt cctctctaag tatttttaat cctggaacta
     1201 aatcatattt agtgtaatct ttatttaaag aatcaatacc ataagatttt gcgttgttag
     1261 gtacattaaa atcgggttct cctatactaa gatttattac tttaactcca ttattttcca
     1321 tttctttaac tttagaacta atacctattg tgtaggatgg tgttataaag tttagtcttt
     1381 ttgataacat aaaatgcctc cttttaaatt attatttata ttatacgaca aatttaaaga
     1441 atttacataa ggaaatttgt agatatatac attaatatat ataatatcct acactttttg
     1501 cctaaaatta gtgtaaaaaa aagagaaaaa aattttgact ttcaaagttg ttagaaacgc
     1561 tactgttcaa aatatccaca tagtatttgc aatgatttgc gcaagtttga ctattatgta
     1621 caaatttggt aatcttaata tagcaattat taagataatc cctttaactt agttgaaggg
     1681 gattttttta gacttaacat actacatata gtggtatggg gattaataaa atacaatata
     1741 tagtaacagg aggttttttt aattggttga attcgtaaaa aaaagagatg gaagagtaat
     1801 tccatttaat gaagatagaa taacaagagc tatattttta gcagcaacga atgttgcaga
     1861 aagagaagga atagtacctg attataaatt atctgagcaa ttaactcaag aagtaataaa
     1921 gtttttaaat cataaatact cagaatcagt tccaagtgtt gaagatatac aagattctgt
     1981 agttaaggtt cttatagaaa ctggccatgc aaaaactagt gaagaatata taatatacag
     2041 aactgaaaga agtagaataa gaaattcaaa aacaagattg atgaaggcaa tagaggaaat
     2101 tacctttgaa gatgcagaag atgctgatat aaaaagagaa aatgcaaata ttaatggtaa
     2161 tacagcaatg ggaactatgt tacaatatgg aagcactgta tctaaagaat tttgtaaaac
     2221 ccatatatta aaaccagaac attcttttgc tcatgataat ggagatatac acatacatga
     2281 tatggatttt ttaaatatgg gaacattaac ttgttgtcaa atagatgtta aaaaactatt
     2341 caatggtgga ttttcaacag gacatggttt tttaagagag ccacaagata taataagtta
     2401 tggagcatta gctgcaattg ctatacaaag caatcaaaac gatcaacatg gaggtcaaag
     2461 tataccattt ttcgattatg gattggctga aggtgtatat aagacattta agaaattcta
     2521 cataggaaat ttagctaaag cgttgaagtt atttaaagga attgaaaata atgatgtgat
     2581 aaagaacata gtatataata cagagaaaga aacaaatcaa aaagttggtc ttaagagaga
     2641 tgaattgtat ctaaacttag aaaaagagaa attaatacag acttttgata tagatgagga
     2701 acttgtaaat aagatgcaaa actttgcatt tgaagaatcg tatagagaaa ctgataaaaa
     2761 aacatatcaa tctatggaag catttataca caatctaaac acaatgcact ctagagcagg
     2821 tgcacaggta ccattttcaa gtgtaaactt cggaacagat acttctgaag aaggaagaat
     2881 ggtaactaag aatcttctat tatctcagga aagaggttta ggtaatggag agacaccaat
     2941 ttttccaatt ttaatattta aggtcaaaga aggcataaat ttaaatcctg aagaccctaa
     3001 ttatgattta tttaaattat catgcagagt atcagctaaa agactattcc ctaattttag
     3061 ttttttagat gcgcctttta atgcaaaata ttataaaaaa ggtgaaccag atacagaagc
     3121 tacatatatg ggttgtagaa caagagtact tagtaatgtg tgtggttcag aaaccgttag
     3181 tggtagagga aacatatctt ttactacagt aaatttacca agattgggta taaaacatgg
     3241 tattataaat aatgaaaagg ctaatttaga tggtttcttt gaagaattag atgagaagat
     3301 aaacttaatt atagaacaat tattggaacg tcttgaggtt caaggaaata aaaagatgaa
     3361 gaattttcca ttcttaatgg ggcaaggtgt atggaaaggt tctgatgatt taggaccaga
     3421 agatacacta aaagaagtaa taaaacaagg aacattgaca attggattta taggtttagc
     3481 agagtgcctt attgctctta taggtaaaca tcatggagaa agtaaagaag ctcaagaatt
     3541 aggattaaaa atagtgtcac atatgagata taaaatggat gaagcaacgg acaaatataa
     3601 attaaatttc tccttaatgg gtactccagc agaaggtctt tcaggtaggt ttactaaaat
     3661 agataaaaaa gtttatggag aaattaaagg tatcacagat aaagaatatt atactaattc
     3721 attccatgta ccagtttatt acaatataag tgcatatgat aaaattgaga tagaagctcc
     3781 ataccatgaa ttgactaatg caggtcatat aacatatgta gagttagatg gagatccatc
     3841 tgataattta gaagcttttg aaactgttat aaaagctatg aaagatttgg gtataggata
     3901 tggaagtata aatcatcctg tagatagaga cccaatatgc ggtttttcag gtgtaataac
     3961 aagtaatata tgccctgtat gtggaagaaa tgaagatgaa agtgacatca aattcgaaag
     4021 aataagaaga ataacaggat acttagttgg tacagttgat agatttaata atgctaaaaa
     4081 agcagaagta agagatagag taaaacatag atagttttat ttgggggaat aagttatgaa
     4141 aataagaatg tcatctacta taagttatga cagcatagtt gatggaccag gattaagaat
     4201 ggtaatatgg actcaaggat gtattcataa ttgtaaagag tgccataatc ctcagacaca
     4261 tgatttatgt ggaggatttt atatggatac agaagaaatt ataaataaag tcaaatcatt
     4321 gaaattacaa aaaggcatta cgctatcagg aggagaacca tttttacaac cagaaccatt
     4381 agaagaaata gcaagagaag ctaaaataaa tgggttagat gtatggtcat atactggatt
     4441 tacgtttgaa caactgttag ataaaaaaaa tagagcgtat tttaaaaatt taaatctact
     4501 aaaacaaatt gatattttag tggatggaaa atttgtaact gaaaaaaaag atataagctt
     4561 aaagtttagg ggttcatcaa atcaaaggat aattgatgtt caaaaaagtt tgaaatataa
     4621 aaaagtattt ttggttgaac aatatatgaa ggatgattta tctatagcag aataaaatat
     4681 aaataaacag gattaaatat atacttatat ttaatcctgt ttattttcat ataatatttc
     4741 aagcttgtat aatgtaacaa ttaaaccaaa atattcattg tatatttgaa attaagtttt
     4801 ttatagttta gaggtgtttt atgtggatta caaagaatat agcatccata cgttatttaa
     4861 tcgattctta tgccaagaaa caattaattt atagaaattc ttaattgtta caatataaat
     4921 atttagacaa gttataagat actgataaat aatttagaaa gtttattaat ctaatattgt
     4981 gtgtgttgat attaagtggt caagtagttt aaaaaatgaa aaagatttaa aataagtgtt
     5041 ttaactaaat acataagtgt taaattcatg tataagtata ggttagatat ataaggaata
     5101 attatgtaat atctatcttt gtaaaaaaat attaaatttt gtaatttgtg gataaatatg
     5161 tttataatgt ggataaaatt taatgtaata agttaaagca atatgaatca actgtttata
     5221 atgtggataa tattataaat gtatgaataa ttgcagtatt taaaaacaaa aaaagttaaa
     5281 aaagttgttg actctaaaag aaaaagttgg tatagtaata cttgtcctca agaaaagggc
     5341 aaacaaataa agca
//
LOCUS       DAEMAE010000232         5313 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEMAE010000232 DAEMAE010000000
VERSION     DAEMAE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.234"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(233..949)
                     /gene="dapD"
                     /locus_tag="KPH93_003235"
     CDS             complement(233..949)
                     /gene="dapD"
                     /locus_tag="KPH93_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG1690008.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1168..1917)
                     /locus_tag="KPH93_003236"
     CDS             complement(1168..1917)
                     /locus_tag="KPH93_003236"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG1690009.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIHEAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(1984..2871)
                     /locus_tag="KPH93_003237"
     CDS             complement(1984..2871)
                     /locus_tag="KPH93_003237"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG1690010.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(2988..3989)
                     /locus_tag="KPH93_003238"
     CDS             complement(2988..3989)
                     /locus_tag="KPH93_003238"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG1690011.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRIVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4419..5300)
                     /locus_tag="KPH93_003239"
     CDS             complement(4419..5300)
                     /locus_tag="KPH93_003239"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG1690012.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFTIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNSGFKF"
BASE COUNT         1742 a          960 c          655 g         1956 t
ORIGIN      
        1 gaaaaattga aggaaataaa aatacttgtc agcaagaaat aaaaatgata taagaatgaa
       61 taagagaatt gtaaaaaaat aaaatgatta tcaataaaag ataaaaatat tataaaaaag
      121 agggtgtctc tggtgaatta aattcataca gagacacctt tttaattaat tttttaaatt
      181 agtatattat tgtttaatta atctttttaa ttaatagtat agtattattt aattagtcta
      241 aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct ttcattttta
      301 taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta gttacaacag
      361 caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata actgcattag
      421 ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc tctaaaacac
      481 cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct ctagctccaa
      541 taacagcatt catatctacc atagaacctt caccaataac agctcctata tttataacag
      601 ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt attgcacctg
      661 gttcaattct tgcatgttca cttaaagtat tttttagagg aatagcagaa tttcttctat
      721 catattctac gtgagtatct gttatatcat ttccatgttt atctaatact tccattatat
      781 cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta aatttatcag
      841 ttgattggaa gtgtaaagaa ttagtattta catatacctt aacaggtgtt ttctttttaa
      901 cttcttttat atatcttgct atttcataag catcgtttaa atttaccatt tgttaattct
      961 ccttgtattt cattatattt cttttattat atacttattt attaatttta gtatatactt
     1021 acttcttatt atttatattt tagttagttt ttattgtata gacatagtat taaataaatt
     1081 tattatatat gtttgatttg tattgcgtat tattatatct atattgcata tgtttaattt
     1141 atattgtgta ttattatatt tatactatta tcccaacatg ctgtccatat tataaaatcc
     1201 tggtttttgc ttaactaagt atttggcagc agctatagaa ccacttgcaa agataccacg
     1261 agattgagct tgatggctta ttgtaagaac ctcattgtct ccagcaaata gagcatcatg
     1321 ttcgccaaca atatttccac ctcttatagc atgaatacca acttcatttg gttgtctttt
     1381 tgcactacgg ccatatctac cataattatt ttcaacgttt ggcaaaacct cttttatagc
     1441 gttagcaatc ataacagctg tgccacttgg ggcatctact tttctgttgt gatgtttttc
     1501 tattatttct atatcaaatc cttctagtaa tttagctgca tctttaacca attttactaa
     1561 tacatttaca ccaagagaca tattatatga ttggaataca ggaataactt tagcagcttc
     1621 atgtattttg ttcatttcgt catcattata tccagtagta gcaattacta aaggtgtttt
     1681 agtctttaaa acgtaagata agatattatc taaattagag tgatgagaaa aatctataac
     1741 tacatcagct tcctcaacta tagtactcat atcttcatag attttaaggt cgccttcata
     1801 gctatccaga cctctagctg caacacaaac caattctaat tcattatctt ctcttatagt
     1861 atcggctaaa atcttaccca tttttccatc ataaccagtt gctataactt ttaacataat
     1921 ttcctcctat gattaagggc taacttttga ccaaacttat tttttaccat gcattaatct
     1981 ttactatgca ttaactttta gtccaaagtt aactaattct ttctttaaca cttctaaatt
     2041 tgctgggtcc atttctgcta gaggtaatct taaatctcca acattgaatc ccaataaatt
     2101 cattgcagtc ttaactggta taggatttac ttctataaat aaagctgcaa ttagagcatc
     2161 cataccaagt tgtaattttc tagaaccttc tatatcgcct tcaaagaatt tagctaccaa
     2221 atcgtgagtt tcttgagggc aaacattagc aagtactgat ataactcctt gacctcctaa
     2281 agataataaa ggaagtatag tatcatcatt accagagtag atagcaaagt catctggaac
     2341 taaactagct atttctgcaa cctgtcctag gtctccacta gcttctttta cagcaactat
     2401 gttatcaatt ttagcaagtt caactataag gctaggactt atatttacac aagttcttcc
     2461 agggacattg taaagtataa ttggaagttt aacactgtta gctattgttt caaaatgacg
     2521 cttaagtcca gctttattag ctttattgta ataaggtgtt attattaata gagcatctac
     2581 tcctaatttt tcagcttctt gacttaagtt aactgagtgc atagtatcat tagaaccact
     2641 tccagctata acaggaactc ttttattaac tttttcaaca acatacttaa tagtagcaag
     2701 ttgttcttca tcagtcatag tattagcttc accagtagta ccacaagata ctatagcatc
     2761 agtaccatta gctatttggt actcaactaa ttcacctaat ttgtcaaaat caactttgtt
     2821 atcctttgta aatggtgtaa ctagagcaac agcagaacct ttaaatatca tataaaatca
     2881 atcccctctt ttttattttt gtgttttata ttacagttac ttctaatgct tgtttttgag
     2941 ttactattaa tattttacaa aattatatat ttatacaagt ttgtatatta tactaaatcg
     3001 tatgataaag ccatttcagc tatttgaatt gcgttagttg cagcaccttt tcttatattg
     3061 tcagcaacaa cccaaagatt tacaccatta tcaacactaa aatctcttct tattcttcca
     3121 acaaatactt catctctatc atcaaattct acagcagttg gatattcgtt tttagcagga
     3181 ttatcaacta ctacaacacc tggagcttct tcaagtgttt ttactaaatc atctaactca
     3241 aatggattgt taaattcaac atttatagat tcactatgac cattttttac tggaactctt
     3301 acagtagttg cagttatttt taaatcgtag ttatcaagta ttttcatagt ttcatttacc
     3361 attttcattt cttctttagt gtatccattt tcagtgaatg aatctatatg aggaagacag
     3421 ttgtaagaga tttgatgagg gtagaatcca gtaggattac cgttcattcc tttctctaaa
     3481 tcttcaacac ctttaacacc agaaccagat acagcttggt aagtagagta aacaattctc
     3541 ttaattttgt atttatcatc aagagcttta agaggaacca tagcttgtat agtagagcag
     3601 ttaggattag ctattatacc cttatgattt tttatggctt ctggattaac ctcaggaact
     3661 actaaaggca catctggatt cattctccat tgactagagt tatctactac taagattccg
     3721 tttgaagcag ctattggagc atacttttca ctaatacttc caccagcaga gaataatgct
     3781 atttgtatgc ctctatcagt aaatgaagtt tcctttaatt cttctactat atattcttta
     3841 ccacaaaaca ttactttaga accagctgat ttagaagaag agaataaaaa tagattttct
     3901 attggaaaat ttctttcttc taatactttt aaaaatgttc tacctaccat tccagtagca
     3961 ccaactattg ctacatttac ttttttcata taaattcacc ccaattttaa attattaaaa
     4021 cattgtttta tttgatttta gttatacaag taagatatgt taatattttt atatatctaa
     4081 aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa aggtatactt
     4141 tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca aaaataagtg
     4201 acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa tattttcttc
     4261 ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat actcttagtt
     4321 acagctacct ctaccctagg ttaataggat attaaaattt gattattcta ataataatac
     4381 atatttaaga aaaaatcaac ttatttatat aaatattgct aaaatttaaa tccagaattt
     4441 gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag aggaagtctt
     4501 aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac tggatttact
     4561 tctataaata aggcagcaat taaagcatcc atatcaagtt gtaatttttt tgagccttca
     4621 atatcacctt caaagaactt agttactaaa tcatgtgttt ctttaggaca tatgttagca
     4681 agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt atcatcatta
     4741 ccggagtata tagtaaagtc tttaggtact aatttagcta tttctgcaac ttgagctaag
     4801 tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc agccacaaca
     4861 gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat tggcaactta
     4921 acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt cttattatag
     4981 taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg actcatatga
     5041 acagacaaca tagtattgtt tgaaccagta cctgctataa caggtatcct cttattaact
     5101 ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt agtcgcctca
     5161 cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata ttcgattaat
     5221 tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac taaagcaaca
     5281 gcagaacctt taaataacat ataatcaaat ctc
//
LOCUS       DAEMAE010000233         5244 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEMAE010000233 DAEMAE010000000
VERSION     DAEMAE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.235"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(5..985)
                     /locus_tag="KPH93_003240"
     CDS             complement(5..985)
                     /locus_tag="KPH93_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG1690013.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMVVDVSHLNEKSFWDLMSIASKPVIASHSNARSLCDAKRNLTDNQLKEISNTDGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASMPSVDGLESTHTAHNIVVELENRGYKKKDIDLISSGNFYRLFRDILK"
     gene            complement(1041..2243)
                     /locus_tag="KPH93_003241"
     CDS             complement(1041..2243)
                     /locus_tag="KPH93_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG1690014.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMIVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2438..2992
                     /locus_tag="KPH93_003242"
     CDS             2438..2992
                     /locus_tag="KPH93_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1690015.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            complement(3107..3898)
                     /locus_tag="KPH93_003243"
     CDS             complement(3107..3898)
                     /locus_tag="KPH93_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG1690016.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEVYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIYKFKDYLNVDGITNFKVAEDIFFYIVVDFSKFNLESIKEYTNV
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMNGTEEILSNILDESCLLNI"
     gene            complement(3926..5014)
                     /locus_tag="KPH93_003244"
     CDS             complement(3926..5014)
                     /locus_tag="KPH93_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG1690017.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYVKSKYNINLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESINYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFVL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDV"
BASE COUNT         1783 a          891 c          563 g         2007 t
ORIGIN      
        1 tatattattt tagtatatct ctaaagagcc tataaaaatt accactggag attaaatcta
       61 tatctttctt tttataacct ctattttcaa gctctactac tatgttatgt gctgtatgtg
      121 tgctttcaag accatctaca cttggcatac tagcgtttcc agtaaaagaa cctatagtct
      181 ctttccctaa gtaatcacta aaatcaaaac caaaagatac atgttctata cctataagat
      241 ttaccatata gtctatatgg tcaaccaaat gtattaaatc ttgcttctta ggattactat
      301 tagtaaatcc tctaaaacta tttattccaa ctaatccatc tgtatttgaa atttctttta
      361 attgattatc tgtaagattt cttttagcat cacataaact cctagcatta gaatgagaag
      421 ctataactgg cttacttgca atagacatta aatcccaaaa tgacttttca tttaaatgtg
      481 atacatctac taccatgccc aatttttcca ttttattgac tgctttttta cctatttcag
      541 ttaaaccacg attttcatcc cctaaacatc ctgtagcaag ttcattctgt tcattccaag
      601 ttaatgatgc atgtcttgca ccaacttctt tataaaaata atctagtaaa tctatatttc
      661 ttccaatatg gcttaatcct tcaagaccaa ttaaaatatt tattttgttt tcacttatac
      721 ctttatctat atctgaatat ttttttacta tatttacaac atctttcata tcttcaattt
      781 catcttttat agagcttata atttgtttaa ctcttttttc aggctcttta tcatatggtg
      841 ggtctatcca tattacaaaa atagctcctt ctatatttgc tcttttaaaa ttatcaagat
      901 ggattcttct aaatacatct ttttccccgt taagccttct tattgttaca tctgcccaag
      961 tatctgaatg tccatcgaaa atcataacaa cctccaatta aataaaaatc tttctaaaaa
     1021 caactttttt tcacgtaaaa ttatatcaaa atatttccta ctaaaagacc aaaataatta
     1081 ccaactatgt atccaaatgt tccaactata agtattggcc ctaccagctt agaccatcct
     1141 tttgatattg ccatagccgc tgctgttgta ggtccaccaa tatttgcatt agatgcaaga
     1201 atagcttctt ctaaactaaa cttaaatatt tttgcaacta taaatgtaac tatcatgttt
     1261 attagtacca ttattccgca gtataaaagt agtaatggtg atttttgaat tattaatgtt
     1321 attgaagcag gaacacccac tacaacaaag aatatgtata ttaaaaatgt acccaactcc
     1381 tgtgctcctt ttatatcacc aaagaaatta ggcataaatg tggcaaacaa catagtcaaa
     1441 gtagttatta ttagatattg atttccaaat aaagtattta acattgctaa aaaaggattt
     1501 gataatggta ttatagcttt aaaccaagtt gatagttcta cagatactgc aacaattata
     1561 aaggcgctac caatggctat tgctatatct tttaaagata tttcttttct tccccaatag
     1621 tttgctgcta ctgtttctcc ttcagttata cctatacttt ccatttcatc tacatgtgga
     1681 tgtttaaaat gttttctaaa aaatccaatt gatggtatgg ctattaagac aaagaaatat
     1741 aatgccatta aaagattatc tgctacaact gttgctgata ctaattctcc tggcgcatca
     1801 aatgcccctg acatagcagc aaagtttaca tttccaccta tataagaacc tgtcatcata
     1861 gctgcaacaa tatttaaatc tggtactaca ttttttaatg ctaaaaatcc aatcatagct
     1921 ccaagtacgg ttcctacaga acctacaaga aatataatga gcaatcttcc actttctttc
     1981 catatctttt taatgttaca ttgatataac aataaagcta tggctaaagg aacaacatat
     2041 ccccatactt ggtcatatac aacagactct actggtataa tatttaaatt tgataataac
     2101 attgctccta caagagctat tattgctccc gacactttcg atgcccaatt gtacttttgt
     2161 tccaagtaaa tactaaatgc tgcccatcca gttacaactg cccataatac ccaaatatta
     2221 tcagggctta caaaactctt catcttaatt acctccattt cattaaatta aaatatcgtt
     2281 atcaatattc aatatattga atattaatac tattataatg catcttttca gaaaattcaa
     2341 tataacataa aaaatacatg cattataaaa ctttttttta ttttattaat ctttataaat
     2401 tatatgttat aatcatgtct gagggggttt gtatctaatg aatatagatg tcggaagcaa
     2461 aattagatct cttagaaaat ctaaaaatat aagtatctct actttagcaa aaaattcaga
     2521 tttaagtaca ggcttaataa gtcaaattga gcgtaatatg gttgttccat ctattgtagc
     2581 aatgtggaaa atatcaaaag ctcttgatat aaatataggt tatttttttg aagagattgg
     2641 aagagaggac tctgatatag tagtaaagaa aaataatcga aaaaagatag taactaatga
     2701 ttctacaaaa ttttacgaac ttcttgttcc taatttatct ggaaaaaaaa tagaatttat
     2761 acttgttaca ctagatggtc atacacaaaa aaacagtgaa tttgtgacac acgaaggaga
     2821 agaatgtggc tatataataa aaggtaaaat gaagataaca cttggaaata aggaatatct
     2881 attggaggaa ggagatagtt tttattttaa cagtactatc ccacatgtgt atgaaaatta
     2941 tgatgatgag gtatgtatct cagtctgggc aatgactcca ccttcatttt aacatgttga
     3001 gttcttactt aaatataaat tgataaaaat ctgaaataaa ttagtatgat aaactttact
     3061 ataatttatt tcagattttt ctagtttatt atttatatat cagtcattat atattaagta
     3121 ggcaactctc gtctaaaatg ttagatagta tctcttcagt tccattcatt ccattgaaag
     3181 aacttgatat aaatgaattt tctatttcat ttttatttat atcaccatgc ttcaatacat
     3241 aggatttatt tgagatatta agcataccat agtccataat agaatctcca actgcataaa
     3301 aatattctat tcctaattct ttagtcaagt attttatagc attttctttt gaaatttctt
     3361 ttggtataaa atatattttt ctgccactta cataaaactt ccaatttttt gattccaata
     3421 catttgtata ttctttaatt gattctaaat taaattttga gaaatcaaca actatataaa
     3481 aaaatatatc ttctgcaact ttaaaattag ttattccatc tacattcaaa tagtctttaa
     3541 atttataaat catactttca atgggctcat agtcagattt tagtttatct attttttcac
     3601 tccatgattt caatatttca ttatctttaa gtataactcc tccatttgag gttatactcc
     3661 aaggaaaata aatcccatat ttattaaact ctatacgctt aaattgctcc actgttctag
     3721 tagttgtagg tataaacata ccataatatt gtatttgctt tattaaattt atagtatcta
     3781 aagatatata agatatttct ttttccctat aaacttctat attagcatat ttactatcag
     3841 catttaaaaa tttattggag taaatgatac tcctgtctaa atctgaaaaa acaatcattc
     3901 aatattctcc tgtctaatat atttcctaaa catctttcat attttttatt attccacaac
     3961 atttataggc ttttaaatcc atttgttcaa cttcaacatt tttctcctta gctaatacga
     4021 atatatggtc taaagattta tcatttaagt ctttaacaag aatcttatac ggaactcttc
     4081 taagtaaaac ccttgtcgtt tctcctacac ctggtttgat aaagttaata tctgttatgt
     4141 tatacttttc ttttatattt aaaacatcaa agtatccatc tcttgttata atatcacttt
     4201 cccagttttc aacttcatca gatatgttct taaattgatt tttaaaacat tcttctatag
     4261 tttcaatata gttaatagac tcatctttat cttttaaatg tctataaaac ttagctccat
     4321 ggaagtcatt tttaccaatt aaatcctctc ttagtacagt tctgcttact agaccagaaa
     4381 cggttgaatt taaacatgca cttggtatta agaaatcttc tctaattcca taaagtcctg
     4441 aatatccagc agggtcagct aatacagcaa gtgttgagtc aaaatgtttt ttaaatttaa
     4501 cattaagctc atcacatgct ttttcaagtt cttttgatat agttccttta ccagtccatc
     4561 catctaaaaa ttgaatcttt gagctcggat gattctttac tatatattca attgcattca
     4621 tatctattcc cttatctctt attatagaga ttgtatagtg aggtaaattt atattatact
     4681 tgctttttac atatcttttt gccaaaatac caataggtgt tcctgctcta gcaagtgaaa
     4741 ctagtactac atcatttcca ttttttttaa ttattttttc acacataacg cctatagaaa
     4801 atgcaagctt atctttattt tctcttagtt tctcatgata taaattcaga taatcttcac
     4861 taacttcata ctctattggt atcatttctg aataatgtct accattttgt atttttaatt
     4921 ctctttcttt atttccttct tcttcaatta aattcgatat atcttttaat aaaaaaatga
     4981 catcatcttt agaatacgtg ctaaaatctc tcatatgact caactcctat cttaaaatcg
     5041 cgatatattt tatcctgtta aaacacttta gctacaacat actatagtta attcttttat
     5101 acttgtcttt ttaattatat caataaattc tagataagaa ttttcacttt tatttagctc
     5161 tacaaataaa taacacttag aaaaattatg tttatctata ttgtaaatat aattttgaat
     5221 atctatatta taaaaactct ttag
//
LOCUS       DAEMAE010000234         5209 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEMAE010000234 DAEMAE010000000
VERSION     DAEMAE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.237"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(45..194)
                     /locus_tag="KPH93_003245"
     CDS             complement(45..194)
                     /locus_tag="KPH93_003245"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1690018.1"
                     /translation="MQKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQNWVKD
                     YKNIG"
     gene            844..1578
                     /locus_tag="KPH93_003246"
     CDS             844..1578
                     /locus_tag="KPH93_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1690019.1"
                     /translation="MNLTKGLDVLKISSNVLGEDKVMYIPVIYTEDEATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNSIKVYAYKSEVSYIT
                     GEETPFKLCMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEILNLGEKMEV
                     IHTGGHTRGHICLYLKESKILIAGDLLQIENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQKNIIETLKNLIIE"
     gene            2562..3818
                     /locus_tag="KPH93_003247"
     CDS             2562..3818
                     /locus_tag="KPH93_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG1690020.1"
                     /translation="MIEKFKTLIWIKMIIRIKIVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFY
                     FTLSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLAKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            3881..5074
                     /locus_tag="KPH93_003248"
     CDS             3881..5074
                     /locus_tag="KPH93_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG1690021.1"
                     /translation="MLTSLALIFLLGMASGGIFKKIKLPSLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGIEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGLCLAVFFKNVHMRDSAK
                     GVLLLSISFLMISLETTLEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRIVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMVSGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
BASE COUNT         1874 a          579 c          881 g         1875 t
ORIGIN      
        1 atatactttc tttctaataa tagatttaat tcctagttct ttcatcaacc tatattttta
       61 taatctttta cccagttttg aacttgcgtt ttgcttctta tattcagcat ctcagcaatt
      121 ttgtatgatg agtatccttc ttttatatat aagtttacag cttttagttt aagttctttg
      181 ctatattttt gtatttttta gacatagaaa aaccacctta gagtatacgc tttttagatc
      241 tattataatg gccttttatt taaatgtcta caataagggg attatactaa aatagagata
      301 agcttctttt tcattgctga aatgacgaaa atataattga tttaatataa aaaagagtgc
      361 tttatgaact atttattttg aacagtagat acacttgcac tcaatttagt tactactaag
      421 ctttatgaac taaaagttta ctttgaatcc tatttttatt aaaagatatt ttatcctttc
      481 taaataatca tctatataca gtattgcgtt tcttttattt attcataatt tattttctta
      541 ttaaagtttt tctatattca tactttctat attactttac ttttaactct atacgaattg
      601 tcaaaaataa aatagttatt agaaaaaaat aaaagtaatt gcaaagcttg caattactta
      661 cttatatact aattactata ccacaaaata acaaaaaaat caagtcgagt aatccctcaa
      721 attaagtgat atactagata ttaataattt ttaattaata aaatataact agaggcaaat
      781 ctatatttct caatttattt aattaaattg acctctaatt aagtaattat aaggagaaaa
      841 attatgaatt taacaaaggg gttagatgtt ttaaaaattt catcaaatgt attaggtgaa
      901 gataaagtta tgtatattcc tgtaatctac acagaagatg aggcaacact tattgacaca
      961 ggtcttccag gtcaaggtga tttaataatt gatgctctta ataaaagtaa cacgagcttt
     1021 gataagttaa aaaatataat aatcactcat catgatatag accatatagg aaacataaat
     1081 tatctaagag aaaaatctaa gaatagtatc aaagtatatg catacaaaag tgaagtcagt
     1141 tatataacag gagaagaaac tccttttaaa ctttgtatgt tggaacaaat ggtagataag
     1201 attgatgata agatgcttag tatgttaaat gttatgggac ttggatttaa atcatcatat
     1261 actaaagtag acgtgtcatt agataaccat gaaattttga atctaggaga gaaaatggag
     1321 gttatacata caggaggtca tactagaggt catatatgtc tttatttaaa agagtctaaa
     1381 atacttattg ctggagattt gctacagata gagaatggag aacttaagcc tgttgatgta
     1441 atgcacagca acaaacaaga gttaaaagat gctattaaaa atataagtaa ttatgatata
     1501 gaaacgatag tttttagtca tggtgggtta tatcaaaaaa acataattga aacattgaaa
     1561 aacctaatta ttgagtagta ctatatgatt tacaataatt tcttaaatta cttaaaattc
     1621 tatctaaaga tgtagaatca aaattaaata taaataataa gaattttgtt ttagaattat
     1681 acttaagggt taagggtgat acgtagtatt acaagtattt tagaatatag ttttttatag
     1741 gaattttaag gttctacaat acttcagttg aaaaatattt aacagcatag tataatagga
     1801 aagaaaatat tttaaataaa ctttagtata caaagtgaag aaaaatattt aaatcatata
     1861 tcatgatgta gagtgaggta aaatatttaa atcatgtatc ataatacaaa gtgaagaaaa
     1921 atatttaaat catgtaccat aatgcagagt gagaaaaaat atttaaatca tacatcataa
     1981 tgcaaagcga agaaaaatat ttaaatcatg tatcataatg caaagttaag aagattattt
     2041 aaagcatgta tcataataca aagtaaagaa aatcatttaa attatatatc ataaaaataa
     2101 tatgaaatat tgaacactca taatattaga ggctttaata ttttaattga taggtcatat
     2161 tatttataac attaaaaata caatttacat aaaatagaca tatgaaaaat aaaaaattat
     2221 acaataatta tgggaatgag gttctccctt ggatgataat atcctaaact gccaatgagc
     2281 tgatgacttc tatgatatca tagatgcacc agcttttttt gttgtttaaa tcgataaaat
     2341 ggaggtgaat tacttactaa atttttattt ttcaaatttg aagaaatatc taattaatag
     2401 aaaaaatagt agattatatg ttaattattt tattaagaat aggttaagat tgtacctata
     2461 tcctatcaat ttaaaaaaca attagatagt cattacattg actacagttg atgcaataaa
     2521 actttcaaaa aatcttatga tgcaaataga aaggaattaa aatgatagaa aaatttaaaa
     2581 ctttaatatg gattaaaatg ataataagaa ttaaaatcgt ttataaaacg tatggtggac
     2641 tatttttgtt aggactcata ggtataccag ttggtgcaat tataggtctt atagatacaa
     2701 tatttggaac ggttcttcta aaagttacag atattcgaga aacatatcca atgtatctga
     2761 taccattttt agcagtagta ggagttgtta ttgcttattg ctattttaaa tttggaggaa
     2821 aaagtagtaa aggaatgaat ctaatatttg aggtaggaca tggagaagaa gaaattattc
     2881 ctttaagatt ggttccattt atcatatcag gtacatggtt aactcattta tttggtggta
     2941 gtgcaggacg agaaggagtt gctgttcaga ttggtgcaac cttttcacat tgggtaggta
     3001 agaggttgcc tataaaaaat gcttctagta tttttctagt aactggtatg gcagctggtt
     3061 ttgcaggatt atttgagaca ccaatagcag caatactatt tgctatggaa gttttggtag
     3121 ctggttcact ggagtatcaa tcgctttttc ctgcgtttac tgcttctttt acagcaagtg
     3181 cagtatcaaa agctttgggg ttggagaaat tttattttac actttcttct aaagttgtat
     3241 ttgacttatc aatattttgg aagcttattg ttttaggaat tatttttgga atggttggtg
     3301 gagcatttgc atggtgcctg aaattggcaa aaagaaaaat aggtaataga ttaaaaaatc
     3361 ctatgataag gattgctatt attggagtat gtcttagtgt attattcctt ttattttata
     3421 aaggaagata ttcaggttta gggacaaatt taattcaaaa tagtttctat ggtggagaaa
     3481 tttattcatt tgactggctg ttaaagttta tacttacaat actgacttta tcagcaggtt
     3541 ttcaaggagg agaggttaca cctttgtttt ccattggagc tagtcttggt gtattgctag
     3601 caggattttt taatttgcca attgaattag ttgcagcgct tggctatgct agcgtctttg
     3661 gcagtgctac aaatactttt tttgctcctg tatttatagg ggcagaagtt tttggataca
     3721 gctatttacc ttatttcttt gtagtatgcg ctatttcata tatatttaat atggataaat
     3781 ctatttattc actacaaaaa atttcaacga aacaataaat aaaacaacat aatgtatgat
     3841 tagaagagaa agaatattag agaaaggata gggtaggaat atgttgacaa gtttagcttt
     3901 gattttttta ttaggaatgg cctcgggagg tatttttaag aagataaaat tacctagttt
     3961 attgggaatg ttacttacag gaatcatatt aggtccatat gttttaaatt tgattgataa
     4021 ttcaatacta gatatttctt ctgaccttag aaagattgca ttgattatta tattaacaag
     4081 ggctggatta tctttagaca taaacgattt gaagaaggtg ggaagacctg ctgtacttat
     4141 gtgctttatt ccagctactt ttgaaattat cggaatgata gttttagcac caaaattatt
     4201 aggtgtttct atattggaag cagcagtcat gggtgcagta gtaggggcag tttctcctgc
     4261 aattattgta cctaaaatgc ttaaattgat ggaagaaggt tatggtatag aaaaaagtat
     4321 tccacagatg cttttagcag gaacttctat agacgatatt ttcgtaattg tgatgtttac
     4381 agtattcaca ggattggcac aaggaaatag tatatcagct attagttttt tacaaattcc
     4441 agtatcaatt atattaggtg taatagctgg agcagtgatt ggactctgtt tagcagtatt
     4501 ttttaagaat gttcatatga gagattctgc taaaggggtt ttgcttttaa gtatctcatt
     4561 tttaatgata agtttggaga ctactttaga gggtattgtg ccattttccg gattattagc
     4621 agttatgagt attgggatat tccttcagat aaaatatcgt gtagttgcaa gaagattgtc
     4681 tataaagtat tcaaaactat gggttggtgc tgaaattctc ttgtttgttt tggtaggggc
     4741 aacagtagat ataagttatg catttaaagc aggaattgga gctgtaattt taatatttgg
     4801 agttctttta ttccgtatag taggagtatt cttctgcctt attaagacaa atttgacaat
     4861 aaaagaaaga ttgttctgta tgattggcta tattccaaag gctacagtac aggcagcaat
     4921 aggaggtgta ccacttgcaa tggggatggt atctggacaa cttattttga cgttagcagt
     4981 tctcgctata cttataactg cacctcttgg agcttttgga attgatgtaa cttataaaaa
     5041 attattaact tctgtaaaag gcgagagtaa gtaaaaataa atattatttt aagtggccaa
     5101 ttatcttttg gaaaaggaat atactattga atacaatata aaaaaatcat ttgggagatt
     5161 aaagaatgta gaagttaaga acaaatttta aggtatgtta taattatta
//
LOCUS       DAEMAE010000235         5159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEMAE010000235 DAEMAE010000000
VERSION     DAEMAE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.238"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            513..1508
                     /locus_tag="KPH93_003249"
     CDS             513..1508
                     /locus_tag="KPH93_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1690022.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSHKIYDGSTERLFNHFQKYKKITVSFSNETNIIFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDAMIEEEDIGSIVERIYMKGSDEYYEQL"
     gene            1486..2349
                     /locus_tag="KPH93_003250"
     CDS             1486..2349
                     /locus_tag="KPH93_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG1690023.1"
                     /translation="MSIMSNYNEASSNSSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKIFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2346..3140
                     /locus_tag="KPH93_003251"
     CDS             2346..3140
                     /locus_tag="KPH93_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG1690024.1"
                     /translation="MIKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3425..4804
                     /locus_tag="KPH93_003252"
     CDS             3425..4804
                     /locus_tag="KPH93_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1690025.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILIFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIC
                     KYKNRYKYM"
BASE COUNT         1846 a          520 c          834 g         1959 t
ORIGIN      
        1 atattataaa tgaaatttaa attcaaccct gaagaaataa gctaaatgta ttagtatata
       61 aatccatact tgaacataaa taacctttat gatatgatat caataataat attatgagag
      121 gagtgccttg atgtcggagt tatataatgg taaccaacaa ttaaatagtt ggagacacat
      181 atattctgta taatcagaaa ttttaagtgt atttttaata catttaattt ttgtgtctta
      241 ttctaccttt atagataact gacatatgac aacaaatagt atttaaaata tatattactt
      301 tgttaaatta tggtgtagta ggctttaatc tgctacactt ttattaattt tgtatagtat
      361 tattttgata actattttga aatagagagc attaattttt ctagtccaca ggagttttct
      421 gtttttgtgc tgttaaatat aagcacagaa aaaggcagat atatacctgt ggactttttt
      481 tatataaatt atgaaatatg gaggtagtta aaatgagcat aattgaactt aatgaactga
      541 ctaaatctta tagaagattt gagaaaaagg caggatttat ggggagtgta aagtctttat
      601 ttaataggaa atatacagaa aaaacagcta tatcgtcatt tgatttaaag atagaagaag
      661 gagaatttgt tggattaata ggtcaaaatg gagctggcaa aactactctt attaagatgt
      721 taactggtat tattgcaccg tcttctggag aaatatctgt tatgggttat taccctaaca
      781 aattagaaaa taattttaaa aaacaatatg ctgttgttat gggccaaaaa agtcaactat
      841 tctttgaatt gacaccagca gatacgtttc ttttatttaa agaattatat gatatatcta
      901 atgaagaata taaaaaaaac ttggattact ttatcgaatt atttgaggta tctgactatt
      961 taaatgttca agttaggacg ctttctttag gagaaagaat gaaaatggaa ctaatagtag
     1021 cccttctaca taatccaaaa atattatttt tggatgaacc aactataggc ttagatgcca
     1081 tagcccaaaa acaaattagg tatttcctaa aagaagtaaa tgaaacaaaa ggaactacta
     1141 ttatacttac ttctcattac atggaagata ttaagagtct ctgtagtaga agtattgttg
     1201 tagaaaatag ccataaaata tatgatggtt ctacagagag actatttaat cattttcaaa
     1261 aatataaaaa aataacagtt agtttttcca atgaaactaa cattatattt ccaaaagatt
     1321 gtaaagttgt tgaacaaaca aatcataaga catcagtaat tgtgccaaaa gaaagaggca
     1381 atgaaacgat taaatggatt tttgataaat atgatgttaa agatgcaatg attgaagaag
     1441 aagatatagg tagtatagta gagagaatat atatgaaagg aagtgatgag tattatgagc
     1501 aactataatg aagcaagttc aaattcttca atgttttata aatttaaaca gagtattaga
     1561 aggtattatg aagtagccaa aattaacttt aaagtgcaac ttatctggag atttgatgtg
     1621 gtggttaata tggtgtttac gattgtaaaa attttatttg cttatatttt atggaaagca
     1681 atttttggtg aaaataaaat agtctctggt tttacattta actctatgtt gtcttactat
     1741 ataataagct catttttagc aggtattgat atgtcaaaga gtaccagtga agagatatgt
     1801 aatcgtgtaa aattgggcac attttcaaag tacatgatta ttccaattaa tgttcaaaat
     1861 tattttattg ctaaaaattt tggaatgaca tgtttttatt ttgtatttaa tatgatttct
     1921 gcatttgtat ggatttttat atttaaaata gattttgcct ttacatcaaa tccagttcaa
     1981 atattaattt caattttgat gatttttcta gggtttattt ttatgatgaa actccacttc
     2041 tttattggga ttttgtcatt taaatttctt gagattagtt tttttagaat gataattgaa
     2101 aatattgttg aatttattac aggaacaatg ataccacttg tactcttacc agaaactata
     2161 gtaacagtta tgaagatatt tccattttat tatacaacat atttgccatc tatgttacta
     2221 ataggtagaa acagcaatga ggcaatacaa ggattaatta ttctagcatt atgggtttta
     2281 tttttttctg tagtaaataa agttatgtat aaaagaatga gaacattatt tgatggggtg
     2341 ggtatatgat taaaaaaaat ttgaaaataa tatttatgtt attaaaaatg aaattatcaa
     2401 agatgatggt atttagattt gatttttttg gagcattttt tgtagatagt tcattattta
     2461 tattacaact tctaatgttt aatagtatat attctcatgt agactcaata gggggatggc
     2521 aacaaggaga gatgttaata tttataggaa ctttctcatt aataaatgcg ataaatatga
     2581 cgattttctt ttttggtatt tatgatattc cacgaaaaat ccaggaagga gaattagact
     2641 actatattac aaaaccatta gacccactat ttaggttaac atttgaaaat attaatattg
     2701 gttcatcacc attaattcta gcaagtatac ttattgtttt atatggaata tctgagttaa
     2761 actcagagat aactttagtt atgataataa tatatatttt catggtgtta ttgatgacat
     2821 tactttttta tgatgtatgt atcatactta gaacagtttc attttttgtt atatcatctt
     2881 ctgcgataat gagattagaa gataatttat tgccaatgaa catgaaaatt cctggtgtaa
     2941 tatatgaagg tgtttttaaa gtattattct atctaatctt accatatgga attatgtcga
     3001 caattccgac tcaaattttg gctgggacaa taacaattaa tggattaata tactcaattt
     3061 taattgtatt tttgtttaca ttgtttatgt ggagattttg gaagttaggt atgagacatt
     3121 ataaaagtgc aagtagttaa taaattggta aaaatagaaa atgtataatt atatgtaaat
     3181 acgtttcata tgacactaaa ctatgtaaac aagtatttgc actaaaggca ctgaaagcta
     3241 taaaatgatt aaagtaagga tttaaaagtt actttagtag aaatataatt gtgtaaaaaa
     3301 gcaagattaa ttaaaactat atccgaataa ttagatataa tttttatgca gaaaggaggt
     3361 ctgtaaatgt ctaattgttt ggaatatatt ttaaaaataa ttaatgtaca gaaaaataaa
     3421 caaaatgagt gatgtatatt attcagaaaa tcatgaacaa gataatcttt atgagcaaga
     3481 taatttactg gcacaaagat ttaatttatt tactctattg aagtttgcat ttcctactat
     3541 ttttatgatg atttttatgg ggttgtatac aatcgtcgat actatatttg tatcaagatt
     3601 tattaataca aatgctctat cagcaattaa tattgtatgt ccaattatta atcttatagt
     3661 tggaattgga actatgcttg ccactggagg aagtgctatt gtagcacgta agatgggaaa
     3721 tggtgatatt gagggagcaa ataaagcttt ctctttaatc gtattatcag gagttgtaat
     3781 tggggtaata attactggta taggtattgt atttatggat tatatcatta tatggttagg
     3841 agcaagtgaa gttttaatta aatattgtcg ttcatatctt tttattatct tgatttttgc
     3901 ctctgcgaat atactacaag tattatttca atgtttattt gtaacagctg gaaagcctca
     3961 atttggatta tggctagtgt taggtgctgg gtgtgctaat ataattcttg actatgtgtt
     4021 cattgttctg ttacaaatgg gtattagtgg agcagcactt gcaactggag taggatacct
     4081 aattcctacc atttcaggta tggtcttctt ttcaaaaaat aaagggttac ttaaattttg
     4141 tatacccaga ctagataaaa gaatattatt tgaagcttgt tttaatggtt catctgaaat
     4201 ggtgggtcaa ctatcaacag cagttactac ttttttattt aatgctatga tgatgaaatt
     4261 aattggtgaa gatggagttg cagctattac aataattatt tattctcaat ttcttttaac
     4321 ttcgctttat ataggtttct caatgggtgt tgcaccaata ataagttata actatggaag
     4381 tgaaaacaat acgcagttaa atcatgtttt taagatttgt atattattta tttcagtagc
     4441 atctttattg gtatttatct tttctatatc atgttcacca atcattgtag agattttttc
     4501 tcaaagagga agccgagttt atgaaattgc aaggtctgga ttttatatat tttcttttag
     4561 ttttattttt tgtgggtata atatttttac ttcgtctatg tttacagcat tatcaaatgg
     4621 aaaggtgtct gcaatattat cttttctacg tacatttgga tttataacta taggtttatt
     4681 gatattgcca aatatattaa atataaatgg gatatggtta gcagtaccaa gtgctgagat
     4741 tttaacttta tttttaaccg ttttttgtat atgtaagtat aagaatagat ataaatatat
     4801 gtagatatta aataaatagt ttaggtagat attttatgtt ttaataagta attcaattag
     4861 tggtagatag atactattga aaattaaagt tataattgat ataaagaatt aataaaaata
     4921 acatataaaa gtttaaaagg ggagttgaac ttaaagtttg attagaaaaa gtcaatttta
     4981 agacaactcc ttttatttta ctattgtatt aatctgtatc tgtaagtgta ttagtctaat
     5041 attataaact ttggatgaaa ttaattttct ataggtttta atgtatcata tactttattt
     5101 taaagaaacg catcttaatt ttcataatga ttgaatttgt atattagaga tgttactat
//
LOCUS       DAEMAE010000236         5152 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEMAE010000236 DAEMAE010000000
VERSION     DAEMAE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.239"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            123..884
                     /locus_tag="KPH93_003253"
     CDS             123..884
                     /locus_tag="KPH93_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG1690026.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKQGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            949..1731
                     /locus_tag="KPH93_003254"
     CDS             949..1731
                     /locus_tag="KPH93_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG1690027.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVIKDKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCELLKDNTVKISGEAVLFMQGAIKIDI"
     gene            2005..2424
                     /locus_tag="KPH93_003255"
     CDS             2005..2424
                     /locus_tag="KPH93_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG1690028.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDKIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(2527..2793)
                     /locus_tag="KPH93_003256"
     CDS             complement(2527..2793)
                     /locus_tag="KPH93_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG1690029.1"
                     /translation="MKNFTLIYFIVINSIAFFSMYIDKKRAVRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3020..3721
                     /locus_tag="KPH93_003257"
     CDS             3020..3721
                     /locus_tag="KPH93_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690030.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERINNLLDISID
                     RVEDYFKDKKVKESEEIVPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDAELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            3803..4411
                     /locus_tag="KPH93_003258"
     CDS             3803..4411
                     /locus_tag="KPH93_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1690031.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSNE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGTKLFE"
     gene            4735..4827
                     /locus_tag="KPH93_003259"
                     /pseudo
     CDS             4735..4827
                     /locus_tag="KPH93_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
BASE COUNT         2114 a          471 c          881 g         1686 t
ORIGIN      
        1 ttatataggt aaattgttga attaagttta tattatttaa ataaactact taatttagat
       61 aatataaaaa gtcagaattt taagaataga gctaaataat cttagttatt aggagaatta
      121 ctatgatagg aatatatttt agtggaacag gaaattcaag atactgtgta gaaaaatttt
      181 tacaagaata tgatataaca gcaaattctt attctattga agataatgaa cttttacagc
      241 atatcaataa tcatgagaat attattttta gttatcctgt acaatatagt aatgtgccaa
      301 aaatattgaa agattttata ataaatcatt caactttgtg gaatggtaaa cgtgttttta
      361 ttattgcaac tatggctcta tttagtggag atggctctgg tgtgttatct agaatattaa
      421 aaaaatatgg tgctataact gttggtggat tacatgtgaa gatgccagat agtatagggg
      481 atgaaaaggt attaaaacat tctttagagt ataatagaaa gcttgtaata aaagcagaaa
      541 agaaagtaac taaggctgct aaaaaactga aacaggggtc accaccaaaa gaagggttag
      601 gttttttgag tcatattgta gggttatttg gacaacgatt gtattttatc aataaaacaa
      661 atatgtatac agataaacta aaaattaata ctgaggaatg tattgtatgt ggtaaatgtg
      721 ttaatttatg tccaatgaag aatctagtaa ttaaaaattg tatgattgtt gcaaatgggc
      781 aatgtacaat gtgttatcgt tgtgtaaaca attgtgctaa aaaagcaatt acattgttag
      841 gtaaaaaagt tgttgaacag agtaatatta aaaaatatat gtaagaaatt taaagtttta
      901 ttgaatgtaa attgatgtat attttatgtt agaaagagag gtcaagtaat ggaatactac
      961 atagttgatt catttgcaac taaactattt aaaggaaatc ctgctggagt ttgtgttctt
     1021 gacagaagaa tacctttaga gttaatgcag aaaatagcag aagaaaacaa tttaccagaa
     1081 acagcatttg taattaaaga taaagggaat tatgaattac gatggtttac tcctaaagca
     1141 gaaattgatt tatgtggtca tgcaacttta gcagcagctt atgtaatatc aaattttatt
     1201 gatgttaatg taaaaaagat agatttcttt actcaaagtg gaaagcttga agttacacga
     1261 aatggaaatc tatatgaaat gatatttcca gaaataatgc ctattgaaat agagttatca
     1321 ccacaacaag ctaatttgat aggctgtgta ccaagtgctg tttattcttc aagagattta
     1381 atcttattgc taaacagtga acaagaagtt ataaattata aacctaatta cgcacaattg
     1441 cgtaaattga cagactggtt aggtataata atcacagcac aaggaagtaa tacagatttt
     1501 gtatcaagat atttttgtcc agaattagat tcagaagacc ctgttactgg ttcatcacat
     1561 tgtaatctta ttccatactg gtctgaaaag ttgggaaaac acaaaatggt agctgctcaa
     1621 ctttctaatc gtggtggtat aattcagtgt gaactgttga aggataatac tgtaaaaatt
     1681 tctggtgaag cagtcttgtt tatgcaggga gcaataaaga ttgatattta aataagtata
     1741 ataactaatt tatgatttat tatgaaaaat aattaatctt cttagagtta ataataataa
     1801 agtagagtta aaaagcttaa tttcacttaa atcaatatga tagatgttga tactaagatt
     1861 gacaagcatg attaaaaaat ataatattta atctataaag ataaaaacac agtctggttg
     1921 gtagtccaga tgcagcaata gctgtcagta acctgcctcc ttggttgtcc gttctttaga
     1981 aagtaattta aggaggaact agcaatggac aaaattagtg gaaaattaac agtcttattt
     2041 gaagaaccat tttgggtagg tatatttgaa agacaagatg gtaaaaaata tgaagcatgt
     2101 agggtagttt ttggagcaga acctaaagaa gtagaagtgt atgaatttat acttgaaaga
     2161 tttttctcat tagattttgg tagcattaaa ttagaaaaaa atgtgaccaa agacaaaatt
     2221 ggatacaaaa gaatgcagag aaaagtaaaa aaggaacaag aaaaagaaac tatagggact
     2281 aaggctcaaa atgctcttaa gcttcaatat gaagaaagaa aacaggatag aaaaaaccta
     2341 gctaaaaata gaaaagaaga agaaaaagaa agactattta atttgaaaca agaaaagaga
     2401 aaagctaaac ataaaggtca ttaattttaa ttaaatatct ttttaataat tgctagaaaa
     2461 aggtgtataa aatttttaat tttatacacc ttaatttatt gaaaagaata aggttttata
     2521 tttcaattac tttaaaatat aaaaatatac taataaaaat tgtaaaaata gtataaatgg
     2581 tataccaaat gtaaatttaa tgtgtttagt tttatgtcta aaagaataca ttccaatcat
     2641 agaacctata ctacctccaa ttactgctat agacataaga gtagcttctt ttattctcca
     2701 ttcgtttcta actgctcttt tcttatctat atacattgaa aagaaagcaa tagaattaat
     2761 cactataaaa tagattaaag taaaattttt cacagccttg cctccataaa aatatattat
     2821 acattataaa acaagatatt tgttttggaa agttaaattt gtaattagta catataaaat
     2881 atttgataag aatcaatgta atattaattg gaaaatttaa aaaaattaaa caattgctaa
     2941 atagggggta tatttataag acacaaagtg ataaaatcta tttagaaaat aatgtaaaat
     3001 aacaggagga taaggcaaaa tgaaatttat agtagaaaaa gaagtttttg ataaattaga
     3061 aaatgtatgt tttggtgtag tagttgcaaa aggaatagat aatactaaag aaatagaaag
     3121 aataaacaat cttttagata taagtataga tagagtagaa gattacttca aagataaaaa
     3181 agtaaaggaa tcagaagaaa ttgtaccata tagagaggca tttagaagtc taggcatgaa
     3241 cccaaataaa tttatgagtt cgatagaagc aatgacaaca agggttgcta agaataagaa
     3301 acttcctcat ataaatccta tcgtagattt aggaaactct atttctttaa aatatttact
     3361 tccaatgggt gctcatgata tggattttag aaatgatgat gtatatgtta gattttctaa
     3421 aaaaggggat aaatttgtac catttggaga aactgatgct gagttaatgg aagaaggaga
     3481 attaatttat tcagttggag acatggtaaa aactagaaga tggatatgga gacaaggaga
     3541 ggaagggaaa attactaata gctcaaaaaa tatatttttt cctatagatg gttttactga
     3601 tgctaattta gataaggtaa tgagtgcaag agaagaatta gcaaaattgt taaaggaaat
     3661 atttaattgt gaaattaaag taggttttgt agataaagat aatcctgaaa tggaaatata
     3721 ataagttgtt gaatatttgt taagatatta tatttattta atgatgttaa aaaggtatta
     3781 aaaaagaaag gtcgtagtag atttgaaaaa aactataata aagaatgtaa atccaatgat
     3841 tgaaaataag tttataggac tttttgaaat agagtataaa aacaaattag atgaagataa
     3901 ggtttggatg gtagcatcaa gaaaaagtaa cgagcaatta aaaaatattt atttgaagaa
     3961 tgaagaagat agtgtagatg cagttgtaat agttggactt cataaaagta gtaaaaaatt
     4021 aattttaata agacaattta gagtacctat taatggatat atatatgaat taccagcagg
     4081 tcttgttgat gaaggagagt ctatagacat ttctgttgaa agagaactta gagaagaaac
     4141 aggacttact cttttagaaa ttaaaaaaaa taaaagtatt gataaagttt atttatctcc
     4201 tggaatgagt gatgaatctg ttgcatttgt ttattgtata tgtgatggtg atataacaga
     4261 tgaattttta gaaccagatg aagagattga agcgctttta atttcgcaag aagatgcaaa
     4321 agagatttta caaagtaatc ataagataga cacaaaagca tttttgattt tacaaatgtt
     4381 tgtgagttta ggaactaaat tatttgaata gatatataga aaagaggtta tatagaaaag
     4441 taaaataata aagtttattt tacttgtata aattataatc tcttttttta tacaataaag
     4501 tataattttg tgtattttta aataagtgac ttgactctta aattttcata atatgttata
     4561 aaccaatttc agatactaca ataattaaaa tcccaaaatt atttaacata tttgcaatca
     4621 aaatgagaaa aggtaaaggc tgaatgattg aaaaatttga tgtgaaatag tagattagtt
     4681 taatattata gtttgtaggg ttaaaaatca gaaattgtaa aggagataaa ttctatgaaa
     4741 aaattagtta ttattttaat tagtatcatt agtgtggtta gcttgacagc ttgtaaaaaa
     4801 agcaaggtta cttttgaaag caatttggtt aaggagaaag aaaaaccctt aaaataaatt
     4861 ccagcttgtc tataacttga tattcagtac ataaaagcag ttaatattta tgattgactg
     4921 ctttttctta tcaaaaagga taggttgtgc atttttactt gaaatagttt atacacatca
     4981 aaagttggtg agcatgtttt ttgaaatggt aagtatgaaa tcttgataac acaaggaaat
     5041 gaatttaagt tttcaattaa ttatatttcc agaagttagt taacaataag ctaattatta
     5101 tttatatttt gatatataaa taataattgt agcaactttt aaaaaatcat at
//
LOCUS       DAEMAE010000237         5147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEMAE010000237 DAEMAE010000000
VERSION     DAEMAE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.240"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(288..1694)
                     /locus_tag="KPH93_003260"
     CDS             complement(288..1694)
                     /locus_tag="KPH93_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1690032.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRVWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELVSEFI
                     NNSLDNDFYIELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQEKINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKIILKLPLIK"
     gene            complement(1739..2446)
                     /locus_tag="KPH93_003261"
     CDS             complement(1739..2446)
                     /locus_tag="KPH93_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1690033.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFKNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTSDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PRHLLTVRGLGYKLMIN"
     gene            2617..3531
                     /locus_tag="KPH93_003262"
     CDS             2617..3531
                     /locus_tag="KPH93_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690034.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPKGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3531..4292
                     /locus_tag="KPH93_003263"
     CDS             3531..4292
                     /locus_tag="KPH93_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690035.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYS
                     SKFLCSAILVSVAVLMQVLSIVLFGHFRGVIESISVFSLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMVAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4304..5041
                     /locus_tag="KPH93_003264"
     CDS             4304..5041
                     /locus_tag="KPH93_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690036.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILISIFCAYICRLEHMNHNWNNVMTLPVKLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTILFSEIANRNFRKNDVIS"
BASE COUNT         1770 a          804 c          679 g         1894 t
ORIGIN      
        1 caataataaa attaaatatt aactaaaaaa taaaaaaaat ggaattatct aaagaattta
       61 ataaccttac aataattcca aaatattatt tctatgaaaa ttttgatacc atgagtaact
      121 cagccataag cgcataaagc acaagttact ctttacttta ataaaacttg acaagaaaag
      181 aatttatcat tattctacta taatagagtt aatttgtcaa taaagaataa agttattttc
      241 aaaatatttt cattcttcat tcttatctgt tattatttta taaaatatca cttaattaga
      301 ggtaatttta gtattatttt tagtccctga ctatcaatac tcgtaatatc taatttacct
      361 ctatgtgcat taataatgcc agatactatc aaaataccta gaccatgatt ttgacctttg
      421 gcatatgcct cacttagtcc attttggctc tttaataaac tattaatttt ttcttgactc
      481 ataccctttc catcatcttc aacaattata tagcaaaaat ctaaatcaga cttagaggta
      541 acccttatat tacatccttg ttggttatga gttatactat tttgtattaa attactaata
      601 gcccttttta ataatttttt atctgcatct ataataatat cttcatttag tatatcaagt
      661 tcaatataaa agtcattatc caaactatta tttataaact cagaaacaag ctctcttaaa
      721 aacgcagaaa ctctaatctt atcacaattc aatgcttgca tattatattc aagtttagac
      781 actaaattga gatcatttac taattctctg attttgaccc cttgattgca tataatagat
      841 acttgctctt gttctctatt tgaaagacta ctgctttctt caagttctcc agcatatcca
      901 attatcattg aaagtggagt tcttatatca tgagatattc ctgcaatcca gttagaccta
      961 gcttcctctt tcttcctaag tttttcatct ttcatttgca actctttaga tacagtatta
     1021 atagattttg aaatatcact tagtattcct ttttcttgta aaagtactgg ttcccctttt
     1081 gacataagct ttataccagt aataattggt tttatagatt tcaccatttt taaacctatg
     1141 aatagagcta ttaaaaaagt aactattgcg ttaaccagaa acatacctat tattaacata
     1201 ggaaattgtt ctatttcctt aatcgtataa gtaaggttaa acttagtata cttatccttt
     1261 ggatagcctg ttactaatat tccatttgga tgttcccata catacacagg ataatccttt
     1321 aaaaagaacc ttgaaaactt tgctacatct gtcaaagtat aatgacttgg aatttctttt
     1381 ggtaaatcaa atccccatac tctatctcca ttgttatcaa ttaacattgc ccaaacttta
     1441 tttttatgta gtacttcctt actagaatca tctaaaataa actttccatc ttttaaaatc
     1501 aaattatctt caatcataat caagttggat tttatttctc tgctatcact tgtatctaaa
     1561 ttatcagagt atttagatac aaatagcata tatgaaaaca gaagaaaacc tgctatattt
     1621 aatacaataa aaagtaatga aatcaagata cttgaaccta taaatcgcct caatatgtac
     1681 tttacactat ccaaaattaa atcactcctc ttgatttctt ttattctaat atctaaaatt
     1741 aattaatcat caacttatat ccaagacctc ttactgtaag aagatgtcta ggtgtagatg
     1801 gagttttttc tatcttctct ctaacccttc taacatgaac cataagggta ttttcatatc
     1861 catacatatc atctccccaa atggcttgac atagtgaatc actagtaaca attctccctt
     1921 tattttcata caactttaaa agcaatgaat gctctttagc tgttaaagaa atctcttgcc
     1981 catctttctc aacacatgca ctatttaaat tcacaacaac agaatctccc agtttaaata
     2041 ctggcaactc ctcttcttct ataggcacat atacacgatt taatataccc acaagtctta
     2101 aagtaagttc ctttggtaaa aatggcttta ctatataatc atcagcccct aagcccaaac
     2161 caatcaatct atcttcatct tctcctcttg cagagagaaa aataactggt atcttagatg
     2221 tctctctaat ttttctcata atagagaaac catctccatc tggcaataca acatctaaaa
     2281 ttgcacaatc tggctttaca tttttaaata ccctaacagc ttcctcataa cttgatgcta
     2341 aaaatactct actaaatccc tcttttctta ggatttcatc tatcattttt aataattcct
     2401 tgtggtcatc aaccacaagt atttttttat cttttattga ctccataatc atcaccctcc
     2461 tttagtatac catataccca ttttcacaac tttgcctaaa tagactttta aggttattgt
     2521 aaggtaaact tcagccaact gaaatgtaac caagttatta tactgatata agctaaaaga
     2581 atcaataaac acagattaaa aaaggggatg aaacttatgg aaatgataat tagaacacaa
     2641 agactttcaa aggaatacaa taaaatattt agagtaaagg atatagacct aagagttcca
     2701 aaaggagctg tgtacggttt tttaggtcct aatggagctg gaaaatctac cactcttaaa
     2761 atgcttcttg gacttgctaa acccacacaa ggaaatatta atattttagg taaagagtta
     2821 aatgaaaaaa atagaatatc aattttaaaa gatataggtt ctttgataga atctccatca
     2881 tactatggtc atctgacagg gcttgaaaac atgactataa tgcaaagact cttagatttg
     2941 cctaaaaaaa atgtaaatga agcattaaaa attgtaaggt tagaaaatca aaaaaataaa
     3001 aaagttagtc aatattcttt aggtatgaag caacgtcttg gcattgctat ggcaataatg
     3061 aaatttccca aattattaat acttgatgaa cctacaaacg gactcgaccc agcagggatt
     3121 gaggaaatta gagaattaat aaaatctcta ccagaaaaat acggaatgac tgttctaatc
     3181 tctagccatc ttttaagtga aattgaccaa attgccacat caataggaat aataaatcat
     3241 ggcgaactgg tttttcaaga tagcataaaa gaacttcata atagaggtca aagtcagatt
     3301 gctatcaaaa ctaagaatgt tgtatctgca caaaaactat taaatactaa aggatatatg
     3361 tcacttatac aagatgggta cttgactatt gaaaatttaa aagactcaga tgtggctaaa
     3421 ataaacggta tgcttgtcca ttcaaatata gatgtattta gaatagaaga acataaaaaa
     3481 agcttggaag atatattcct agaacttact ggtaaggcgg tgactttata atgaaatacc
     3541 ttggtctaga attttataaa cttaaaagaa agaagattat tctgatgacc tttttatttg
     3601 tagctgttga aataatgtgg tgtatcgtaa attcaaatag ggctattaca cgtaatccag
     3661 atatgttagg tggatttgaa tattcctata tgcttatgag ttttgcaagt ttaaatggtc
     3721 tattctttcc tatattaata tcaataatta catcacgtat aagcgatata gaacataaag
     3781 gagatacatg gaagctttta aaatcttctg taacatcttt aaatagtatt tattcatcta
     3841 aatttttatg ttctgctatt ttggtatctg tagctgtatt gatgcaagtg ttatctattg
     3901 tgttgtttgg acattttaga ggggttattg agtcaatttc tgtattttca cttttaaaat
     3961 acacattagg aacaattctt gtaagtattg ctatcattgc actacaccta tggattgcaa
     4021 ctgtaattcc aaatcaaatg gttgcaatta catttggcat gattggtagt tttataggtt
     4081 tgacatcagg gttgttttat caaggtattc gtagattatt tatatggtct tattacctag
     4141 agttatcacc tttaagttac gcctatgata atgttttagg ttcatctgtc tatagaattg
     4201 atatgaattt ttctattacg attttagttt tcttaattgg aatgctaatt tattatattg
     4261 gaagaaatca tttattaaaa aaagaagtat aggagggatt taaatgttaa ggaagtcagt
     4321 tttgggagaa attctaaagt taaaaaattc ttttatatgg tatgtattat tagctttgcc
     4381 acttataagt gtattgattg gttctggtaa tttttatcta aatcaaggca tactcaaaaa
     4441 agaatggtac agcttatgga ctcaagttag tcttttttat ggggaatttt tctttcctat
     4501 cttaatctca atcttttgtg catatatttg taggcttgaa catatgaatc ataattggaa
     4561 taatgtaatg acactgccag ttaagttgaa aaatatcttt ttatctaaac ttacagtagt
     4621 ttcaatactt acagctatta cacaagtttt ctttgttatc ttttatatag ttgcaggaaa
     4681 tatgtttggt ttttctggta gtgtacctaa agaattgact ggttggattg ctaaaggttg
     4741 gattatttct atttcaatag cttctataca gttatacatg tctataaaga ttagaagttt
     4801 tgcaactcct attggtatta gtctttgtct ttctctattt ggtatgggat taatagtagc
     4861 aaaacctgct gttggtatat ttttccctta ttctatgtta ggtagtgcta tgggagttat
     4921 aaagcaatct gctttaaatg tcaatgacac tatagttaca atatgtgttg catttattac
     4981 tactatatta ttctctgaaa ttgccaatag aaattttaga aagaatgatg ttatttctta
     5041 atggtaaacg ctagatattt ttttgtttaa aatttatatt ctttttagtt gaaatatata
     5101 tcctttttag ttaaaaatct atatctttta ttttaatttt atatgtt
//
LOCUS       DAEMAE010000238         5131 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEMAE010000238 DAEMAE010000000
VERSION     DAEMAE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.241"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..718
                     /gene="tcdR"
                     /locus_tag="KPH93_003265"
     CDS             164..718
                     /gene="tcdR"
                     /locus_tag="KPH93_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG1690037.1"
                     /translation="MQKSFYELIALVRNNSVDDLQEILFMFKPLIKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFAEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NVLNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVILYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1049..>5131
                     /gene="tcdB"
                     /locus_tag="KPH93_003266"
                     /gene_synonym="toxB"
     CDS             1049..>5131
                     /gene="tcdB"
                     /locus_tag="KPH93_003266"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG1690038.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDTYIDTYKKSGRNKALKKFKEYLVIEILELKNSNLTPVEKNLHFIW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIIESASNDTLESFREN
                     LNDPEFNHTAFFRKRMQIIYDKQQNFINYYKAQKEENPDLIIDDIVKTYLSNEYSKDI
                     DELNAYIEESLNKVTENSGNDVRNFEEFKTGEVFNLYEQELVERWNLAGASDILRVAI
                     LKNIGGVYLDVDMLPGIHPDLFKDINKPDSVKTAVDWEEMQLEAIMKHKEYIPEYTSK
                     HFDTLDEEVQSSFESVLASKSDKSEIFLPLGDIEVSPLEVKIAFAKGSIINQALISAK
                     DSYCSDLLIKQIQNRYKILNDTLGPIISQGNDFNTTMNNFGESLGAIANEENISFIAK
                     IGSYLRVGFYPEANTTITLSGPTIYAGAYKDLLTFKEMSIDTSILSSELRNFEFPKVN
                     ISQATEQEKNSLWQFNEERAKIQFEEYKKNYFEGALGEDDNLDFSQNTVTDKEYLLEK
                     ISSSTKSSERGYVHYIVQLQGDKISYEAACNLFAKNPYDSILFQKNIEDSEVAYYYNP
                     TDSEIQEIDKYRIPDRISDRPKIKLTFIGHGKAEFNTDIFAGLDVDSLSSEIETAIGL
                     AKEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSMSQDSIIVSA
                     NQYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPEL
                     STLLQEIRNNSNSSDIELEEKVMLAECEINVISNIETQVVEERIEEAKSLTSDSINYI
                     KNEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVET
                     EKTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEY
                     NSSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGL
                     PIIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIAS
                     GFSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKVMM
                     PQDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIY
                     DVLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGE
                     FYWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGS
                     GGTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESD"
BASE COUNT         2095 a          524 c          829 g         1683 t
ORIGIN      
        1 gaatattatc aaattaatta ttaaattgat gtatttataa ttgatatttt cgtataaaac
       61 ttaatttatt ttctaattct ataattgtaa tgactgattt gatgtgtatg ttgtcaaaat
      121 ttttaaaaaa atcatcatta taaatataag aggggatgat tttatgcaaa aatcttttta
      181 tgaattaatt gctttagtaa gaaataactc agtagatgat ttgcaagaaa ttttatttat
      241 gtttaagcca ttaataaaaa aacttagtag agttttacat tatgaagagg gagaaacaga
      301 tttaataata ttttttattg aattaataaa aaatattaaa ttaagtagct ttgcagaaaa
      361 aagtgatgct attatagtca aatatattca taaatcatta ttgaataaga cttttgagtt
      421 gtctagaaga tattctaaaa tgaagtttaa ttttgtagaa tttgacgaaa atgtcttgaa
      481 tatgaaaaat aattatcaaa gtaagtctgt ttttgaggag gatatttgtt ttttcgaata
      541 tattttgaaa gaattatctg gtattcaaag aaaagttatt ttatataaat atttaaaagg
      601 atattctgat agagaaatat cagtgaaatt aaaaatatct agacaagctg ttaataaggc
      661 taaaaataga gcatttaaaa aaataaaaaa agactatgaa aattatttta acttgtaatt
      721 aatgtattta aagaagtatt tacaatagaa atcaaatttt agaattaact ttattataaa
      781 atcgataact ttatctaaga atatcttaat ttatatattt tatatacaag aatgtttaca
      841 tatttatttc aggcaacatc tttattcaat cgaagagcaa attaatcagc tgagtatctt
      901 caatttaaga tgttaggaag tgaatgtata tgaaaaccta agtagatatt agtatatttt
      961 atacatagaa aggaggatat ataaaagagt tttagcattt agatgtaaaa atatttaata
     1021 aaaatattat agtaaaggag aaaattttat gagtttagtt aatagaaaac agttagaaaa
     1081 aatggcaaat gtaagatttc gtgttcagga agatgaatat gtagcaatat tagatgcatt
     1141 agaagaatat cataatatgt cagaaaatac tgtagttgaa aagtatctaa aattaaaaga
     1201 tataaacagt ttaacagata cttatataga tacatataaa aaatctggtc gaaataaagc
     1261 cttaaaaaaa tttaaagagt acttagttat agagatatta gaattaaaaa atagcaattt
     1321 aactccagtc gagaaaaatt tacattttat atggattgga gggcaaataa atgatactgc
     1381 tattaattat ataaatcaat ggaaagatgt aaatagtgac tataatgtta atgtttttta
     1441 tgatagtaat gcatttttga taaacacatt gaaaaaaact ataatagaat cagcatcaaa
     1501 tgataccctt gaatcattta gagaaaattt aaatgatcct gaatttaacc acactgcatt
     1561 tttcagaaaa cgtatgcaaa taatctatga taaacagcaa aatttcataa attactataa
     1621 agctcaaaaa gaagaaaatc ctgaccttat aattgatgat attgtaaaga catatctttc
     1681 aaacgagtat tcaaaggata tagatgaact taatgcttat attgaagagt cattaaacaa
     1741 agtcacagaa aatagtggaa atgatgttag aaactttgaa gaatttaaaa ctggagaagt
     1801 attcaattta tatgaacaag agttagtaga aagatggaat cttgctggtg catctgatat
     1861 attaagagtc gctatattga aaaatattgg tggagtctat ctagatgttg atatgttacc
     1921 aggaatacac ccagatttat ttaaagatat aaataagcct gattcagtaa agacagctgt
     1981 agattgggaa gagatgcagt tagaagccat aatgaaacat aaagaatata taccagaata
     2041 tacttcaaaa cattttgata cattggatga agaagttcaa agtagctttg aatctgtttt
     2101 agcttctaag tctgataagt cagaaatatt tttaccacta ggagatatag aggtatcacc
     2161 tttagaagta aaaattgcat ttgccaaagg ttctattata aatcaagctc taatttctgc
     2221 aaaagattca tattgtagtg acttactaat aaaacaaatc caaaacagat ataagatact
     2281 gaatgatact ttaggtccaa ttattagtca aggtaatgat tttaatacta caatgaacaa
     2341 ttttggtgaa agtttgggag ctatagctaa tgaagagaat ataagtttta tagcaaaaat
     2401 cggaagttat ttaagggttg gattttatcc tgaagctaat actacaatta ctttaagtgg
     2461 tcctacaata tatgcaggag cttataaaga tttattaaca tttaaagaga tgagcataga
     2521 tacttctata ttgtcatctg agttaagaaa ttttgaattt cctaaggtta atatatctca
     2581 agcaacagaa caagagaaaa atagtttatg gcaatttaat gaagaaagag ctaaaattca
     2641 atttgaagaa tacaagaaaa attattttga aggtgcactt ggagaagatg ataatcttga
     2701 tttttctcaa aatacagtaa ctgacaaaga atatctttta gaaaagatct cttcatcaac
     2761 gaagagttca gaaagaggat atgttcatta tattgttcaa ttacaaggag ataaaattag
     2821 ctatgaagca gcatgtaact tatttgcaaa aaatccttat gacagtatac tatttcaaaa
     2881 aaatatagaa gattcagaag tagcatatta ctataatcct acagatagtg aaatacaaga
     2941 aattgataag tatagaattc ctgatagaat ctctgataga cctaagatta aattaacatt
     3001 cattggtcat ggcaaagctg aatttaatac tgatatattt gcaggtcttg atgtagattc
     3061 attatcttca gaaatagaaa cagcaatagg tttagccaaa gaggatattt ctcctaaatc
     3121 tatagaaata aacttactgg gatgtaacat gtttagctat tctgtaaatg tagaagagac
     3181 ttatcctggg aaattattac ttagagttaa agataaagta tcagaattaa tgccatctat
     3241 gagtcaagac tctattatag taagtgcaaa tcaatatgaa gttagaataa atagtgaagg
     3301 aagaagagaa ttattagacc attctggtga atggataaac aaagaagaaa gtattataaa
     3361 ggatatttca tcaaaagaat atatatcatt taatcctaaa gagaataaaa ttatagtaaa
     3421 atctaaaaat ttacctgaat tatctacatt attacaagaa attagaaata attctaattc
     3481 aagtgatatt gaactagaag aaaaagtaat gttagcagaa tgtgagataa atgttatttc
     3541 aaatatagag acacaagtgg tagaagaaag aattgaagaa gctaaaagct taacttctga
     3601 ctctattaat tatataaaga atgaatttaa actaatagaa tctatttctg atgcactatg
     3661 tgacttaaaa caacagaatg aattagaaga ttctcatttt atatcttttg aggacatatc
     3721 agagactgat gaggggttta gtataagatt tattaataaa gaaactggag aatctatatt
     3781 tgtagaaact gaaaaaacaa tattctctga atatgctaat catataactg aagagatttc
     3841 taagataaaa ggtactatat ttgatactgt aaatggtaag ttagtaaaaa aagtaaattt
     3901 agatactaca cacgaagtaa atactttaaa tgctgcattt tttatacaat cattaataga
     3961 atataatagt tctaaagaat ctcttagtaa tttaagtgta gcaatgaaag ttcaagttta
     4021 cgctcaatta tttagtactg gtttaaatac tattacagat gcagccaaag ttgttgaatt
     4081 agtatcaact gcattagatg aaactataga cttacttcct acattatctg aaggattacc
     4141 tataattgca actattatag atggtgtaag tttaggtgca gcaatcaaag agctaagtga
     4201 aacgagtgac ccattattaa gacaagaaat agaagctaag ataggtataa tggcagtaaa
     4261 tttaacaaca gctacaactg caatcattac ttcatctttg gggatagcta gtggatttag
     4321 tatactttta gttcctttag caggaatttc agcaggtata ccaagcttag taaacaatga
     4381 acttgtactt cgagataagg caacaaaggt tgtagattat tttaaacatg tttcattagt
     4441 tgaaactgaa ggagtattta ctttattaga tgataaagta atgatgccac aagatgattt
     4501 agtgatatca gaaatagatt ttaataataa ttcaatagtt ttaggtaaat gtgaaatctg
     4561 gagaatggaa ggtggttcag gtcatactgt aactgatgat atagatcact tcttttcagc
     4621 accatcaata acatatagag agccacactt atctatatat gacgtattgg aagtacaaaa
     4681 agaagaactt gatttgtcaa aagatttaat ggtattacct aatgctccaa atagagtatt
     4741 tgcttgggaa acaggatgga caccaggttt aagaagctta gaaaatgatg gcacaaaact
     4801 gttagaccgt ataagagata actatgaagg tgagttttat tggagatatt ttgcttttat
     4861 agctgatgct ttaataacaa cattaaaacc aagatatgaa gatactaata taagaataaa
     4921 tttagatagt aatactagaa gttttatagt tccaataata actacagaat atataagaga
     4981 aaaattatca tattctttct atggttcagg aggaacttat gcattgtctc tttctcaata
     5041 taatatgggt ataaatatag aattaagtga aagtgatgtt tggattatag atgttgataa
     5101 tgttgtgaga gatgtaacta tagaatctga t
//
LOCUS       DAEMAE010000239         5113 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEMAE010000239 DAEMAE010000000
VERSION     DAEMAE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.242"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1247)
                     /locus_tag="KPH93_003267"
     CDS             complement(<1..1247)
                     /locus_tag="KPH93_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1690039.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGAVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYDVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAIIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSYIGVKNDDFKA"
     gene            complement(1469..2233)
                     /locus_tag="KPH93_003268"
     CDS             complement(1469..2233)
                     /locus_tag="KPH93_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690040.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTTMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(2227..2952)
                     /locus_tag="KPH93_003269"
     CDS             complement(2227..2952)
                     /locus_tag="KPH93_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690041.1"
                     /translation="MDKNINNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGIKPSIETKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEVIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(2954..3322)
                     /locus_tag="KPH93_003270"
     CDS             complement(2954..3322)
                     /locus_tag="KPH93_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1690042.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(3497..5041)
                     /locus_tag="KPH93_003271"
     CDS             complement(3497..5041)
                     /locus_tag="KPH93_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1690043.1"
                     /translation="MRNLQKKLIFFLFICIILQITYTTPVYSLCKLYKNNSVNNFESI
                     VSNNQFISKYAIQIILIMTIICIMLISYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNTFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDMFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFELLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         1796 a          861 c          475 g         1981 t
ORIGIN      
        1 atagccttaa aatcatcatt ttttacccct atataagatg atactatagt tataatactt
       61 aatactataa atactacaaa acctttttta tatccatcaa ctatattata tgaatttact
      121 tgtaaagaga tagctataat caaattaata attccacttc ctataaattc aaaagtatta
      181 ataacactag ttgccagtcc agaataaatt tctttatttt tatacttggc atcattaaaa
      241 acctgtagat gtgacataac tatacatccc ataataaaga atgatattgg taaaactata
      301 attgggggtt ttacctggta tacaactaca attacaatcc atataaacaa atctattatt
      361 gccccaaatt ttattatatt aaatttacta gaccttattt tagcaaatac aaaatccata
      421 attattgaac caaatataaa tccatatgta aaaaatgata ctataaatgc agaaaaactc
      481 ttactcacat cataaacgtc cattatgtat cttactcccc acaagcttat aaatgcactt
      541 gtaagaccca caaaagagaa cattatcatt gaattatacc atgttgattt gttttttata
      601 acagacttaa taccatcaac aatattaacc ttctctgact tttcataggg ttcagtatct
      661 acattgaatc catactcttt tggtgtatct ctaacaataa tatacataaa acatccaatt
      721 acagcaccaa ttataccgat taataaaaat gaatttctcc aaccaacaag ttcagacaaa
      781 aacacaagtg gaaaagtagc aagaactccc cctaaatttc ctacaaatga aaatttagct
      841 gttgcagaag caaattcgga cttattgaac catctccctt gaatttttaa aatacaaagt
      901 aatattactg atgttcccgc ccctacaatt acacgagaaa tatatgctaa ttctatactt
      961 tgaatcaaac caaataaaat tgaccctaaa cttgccatta ttattccaag actgctaatt
     1021 tttcttgctc catatttatc aatcaaaatt cctgcaggta tttgcataaa tgcatacgca
     1081 tataggcaga aggatgctat atttgaaatt tgtatagaat taaaacctaa ctcatttgct
     1141 aaattgtctg aaacaacagc agttgacatc cttaaaaata atactaaaac aaatgaaaat
     1201 gtaattatac cccaaacact ccacttagcc tttgtataac ttttcatatc ttctccctca
     1261 aatccatata tttttaaatt ttcttgatgt aacttatata aagtgtatcg ttattcttac
     1321 aaatttttac acgcaatatc attatacaac atatgtatat atttaactag aaataaaacc
     1381 ttatttatat tatatacatg aattaactac ataaataaca agagccatct tttaacatta
     1441 ctcaaaagat ggctccatat aaaaacattt attctaaatt taatttttta tctaatataa
     1501 attttgtagc gaaaaataat gctattgcaa ctacaaggct accaagtatt gcaagcataa
     1561 aactaggact ctgataaaga tgatatacca tagttgtatc ttcatttggt aaaatacttc
     1621 caactgcatc tccaacaatt gatattacta tatttatagc tataaaagaa gcaaatgcca
     1681 ctatgtttct atgtttatta aattttggca attgtcccat tgagattgat gtatataata
     1741 acaaaataaa tgatgaatat gaaataaaca ttactactac taataataaa atatctaaaa
     1801 agttttcaga aatccattta aaactagtgt taaacaaatc taaaatttca ggtagtaaaa
     1861 ttccagaagt accaaaaagc ataagtactc caaaagtaaa tactgcaact ataaaactaa
     1921 gtacagcgta tataagtgct gttatacatt tagataatat aagtgaacta gtgctaacag
     1981 gaagagtaaa cattaaataa ccctcatccc ccaaaagatt ttttctaaac ctttgaattg
     2041 taactacaat agttaagact cctaatgcca taaaaagact tgttaatact agtattccta
     2101 ttccctgtac ctgaagtata tttgtattca taaatattcc attaaataca gctactatca
     2161 atatggctat atataatggt ataaatattc ttccacttgc ttttaactca tattttaata
     2221 atttacctaa catttaaatt cctccctaaa taatgcatct atagattttc ctttttcttc
     2281 tcttattgct tcaacgtctc cttgtaacac tatttctcct tttgatatga atattacctc
     2341 gtcacatatg ttttctattt cacttattaa atgggtagca atcagtaaag tactatcttc
     2401 agaataattt tttaatatag tcttaagtat atatgacctt gcagctgggt caacacctcc
     2461 tataggttca tccaatatat atatgcttgc atttcttgac attactaaga taagttgaac
     2521 tttttcttta gttcctttag acattgtctt tagcttttca tttacatcaa tatctaaact
     2581 tttaatcatc tcatttgctc ttctaacatc aaagtcacta taaaaatcat agaaaaactt
     2641 aagtaaatct gataccttca tccaatcatt taaataagtt ctttctggta aatatgaaac
     2701 tattttttta gtctctatgg atggtttaat tccatttatc ataatttcac cattatcagg
     2761 ctgtaacaat ccattcatta gcttaatcat tgtactttta ccacttccat taggtcctaa
     2821 cagcccgact atttttccct ttggtatatt taaatcaata ttttttaaaa cattcttagt
     2881 tccataactc ttgtttacac ctttaaattc aactattcca tcttgagaaa cattgtttat
     2941 atttttatcc attttataat tctcctttca aattttctgt aataagtttt aacatttcat
     3001 ctctatcata tccaagcttt tctaaagtta cttttaatgt atttatctcc acattagcta
     3061 tttcttcttt catagctttt attttttctt tatcatctgt tacaaatctt cctttagttc
     3121 tctgactata aactaaacct tgtctttcaa gttctgttag agccttttgc attgtatttg
     3181 gattaacttc agcatcttct gctagtgctc tcactgtttc taatttagat cctattttaa
     3241 tttcccctga aattattttt aactttaaat gttctactaa ttgtatatat ataggtttgt
     3301 tattatcaag ttcccattcc atgttcatgc ctccttatta tattattgta ttaatttgtt
     3361 aatacaataa tataataata caaagtatat gtcaattact ttttaataaa agtataaaaa
     3421 attttacaaa aaaatgaaca ctacattaga gtccattttt aacaaaattt attaaattct
     3481 ttatattaca gattaattac cctctcacca aattagatac aattaaatgt tcaaattctt
     3541 caattggcat tggctttcca aacaaatatc cttgtgccat atcacatttt acttccttta
     3601 aaagttcaaa ctgttcttct gtctcaactc cttctgatat gactttcata cccaactctt
     3661 ttgccatttc tattacttta gtaacaatta ttttgcttct tttactatta taagtttcca
     3721 taataaattt ttggtcaagt tttaagatat ccataggaat ttcttttaac atatttaatg
     3781 atgagtaccc tgaaccaaaa tcatccatag atattaaaaa tcctattttt tttaaattat
     3841 tcattatatc aattaaaata tcaaaattat caaatatgat actctccgtt aattcaagtt
     3901 ctatatactt tgcaggtata ttgtatttat caattatatt tttgtatgtt tctataaaat
     3961 tattgcaata taaatgtatt cttgaaacat taactgaaac ggtaactgga tttattcctg
     4021 catcaatcca ttttctaagg tatatacata cttcttcaag tacatataaa tctaaattta
     4081 taacaaaacc gtttctttca aaaaatggga taaattcgtt tggaggtatt agtcctttat
     4141 caggacgttt ccacctaaca agtgcttctg aaccttgtat ctctcctgta ttaagttcta
     4201 tcttaggttg taagtatata ataaactctt tattttctag acttgaaaac attgtatttt
     4261 ccatctcttt ttcttttatc tcttggtcat gaatcttatt atcataaaat gcatatgaac
     4321 ttttatgtcc tccctttgcc attttatgag gtatattagc cctatctatg attgagttga
     4381 tatctctatc ttctggaagt ataaaataga ttccacaact taaaactaac ttatattttt
     4441 cttcttgatt attattaaac atgtctaatt cttcaaatat agtttttaat ctctttgtta
     4501 tatcttcttc ttctatatat ttcataatta taataaaatt atccgcagat attcttgcaa
     4561 acatttcttc ttcaaatgta ttactcttaa taatctttgt taaattacgc aaaatttcat
     4621 tcccaacttc atatccaaat aaatcattta tgtatttaaa tttatcaata tctatatata
     4681 ataaagcata ctttacttga gtatttttac atagtatttt gttagcatca attacaaatt
     4741 tatctatagt atttgctcct gtaagattat ctgtatatgc tatcttttgc aatttaattt
     4801 taaaatttaa tttatcatat attatatatg atataagcat tatacatatt atagtcataa
     4861 taagtattat ttgtattgca tatttgctaa taaactgatt attagacaca atagattcaa
     4921 agttatttac ggaattgttt ttatagagtt tgcataaaga atagactggt gttgtgtatg
     4981 taatttgtaa aattatacaa ataaataaaa aaaatatcaa ttttttttgt aaattcctca
     5041 taaaaatttc ctcccattaa aacacctcta ttttattata ttttattagc aaataagtac
     5101 atctcaaaag ttt
//
LOCUS       DAEMAE010000240         5074 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEMAE010000240 DAEMAE010000000
VERSION     DAEMAE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.243"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            33..644
                     /locus_tag="KPH93_003272"
     CDS             33..644
                     /locus_tag="KPH93_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG1690044.1"
                     /translation="MINDIKNKFKDYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            942..1280
                     /locus_tag="KPH93_003273"
     CDS             942..1280
                     /locus_tag="KPH93_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG1690045.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1687..3123)
                     /gene="miaB"
                     /locus_tag="KPH93_003274"
     CDS             complement(1687..3123)
                     /gene="miaB"
                     /locus_tag="KPH93_003274"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG1690046.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENSVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDKIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3371..4807)
                     /locus_tag="KPH93_003275"
     CDS             complement(3371..4807)
                     /locus_tag="KPH93_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG1690047.1"
                     /translation="MKFYKRILALTLSISFVFANIQSVNAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETYARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYAIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMI
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEHIIMSNSSDAYYEVRNDSLGNADGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1710 a          767 c          555 g         2042 t
ORIGIN      
        1 aatttttaat aatattattt taggaggtaa aaatgataaa cgatattaaa aataaattca
       61 aagattacaa accatatata aatggataca agaatatgaa gagagcatct gttttaattc
      121 caatagtaga aataaataac acacactata tattatttga agttcgctca aaaaatttga
      181 agcatcaacc tagcgaaatt tcttttccag gtggtaaaat agaatcaggt gagactccat
      241 atgaagctgt tattagagaa acttgtgagg aacttggaac ttttagtgac aatatagaag
      301 taatatcaag tcttgacctc cttataacac cagtaaagtt tatcatacat ccatacttag
      361 gatatataaa aaatattaat aatttaaata taaacaagga tgaagttgac catacttttt
      421 tggttcctat aaaatatctt ttagagaatc ctccaaatac ttatagtaat actgtaaaag
      481 tttttccaga tgaaaatcta ccatatggtt caattccaaa ccaaaaagat tataaatttg
      541 aaagtggaag ttatcctatt ctgttttata aatataaaga ttatgtaatt tggggaatga
      601 cagcaaaaat actagaaaac tttttaaatt ttttatcaga ataacactca taataaatca
      661 ttaaaacttt tacataagaa atttataaaa gtttctaaat ttaacaattc taaaatatca
      721 ggaactatat tttgtagcac ttttttgttt tattattgtt tgaatatttg atggttttca
      781 atgcaatttc aacataaata ctatgtatta gtgtatactg ttgatgaatt aaagcatgat
      841 aatctattta ataaaatatg aagtaaatga ttttaatgtg tctttatggt aaaattacta
      901 ataagtaata aaattaatta aaatatatgg aggtgcttat aatgtttgct gtaggatttg
      961 ttgttataat tgcacttgcc tttttcattt taagtgtact attatttaat ggaaaagcca
     1021 gtatgctact agcaggttat aactctatga gtaagaaaga aaaagcacga tacgataaaa
     1081 aaaaattatg tagaagttct agcattgtta cttttattgt ctctataatg ctatttatta
     1141 tggcattttt gggatataga atagagagtg gtcaaatgga cgaaaaaaaa atggttccct
     1201 tttctataat atttataata gtcatacttt ccagtgtatt ttttaatatc ctctactcta
     1261 acaaaaaatg taaaaaataa atagttgtaa gttgagcagt taattataaa agattgactg
     1321 ctttttcatg tctattgact actcttatta aatattttaa catttaaaaa tctatgtaaa
     1381 aataatgaat atagtacaaa tttctaataa tgattttacc tttgtggtta cagtccacta
     1441 tatataattt aatatgataa cagggaatta ctaatgacaa ctaagtaagc ttttataaaa
     1501 tataataaaa caaaaataga tgtacaatga aattatatct tatatcaaat ttatttttat
     1561 taatagcaga aacttacatt agcataacat aagacaaatt cgcttaaaat aaaaattttt
     1621 aatataattt gttctatagt acatctattt tgattagttt acataatttt attattttgt
     1681 acaatattaa ttatttacta gtatcccatt taatgaaaaa gttctaggtt cagtaatttt
     1741 gaccataact aatttaccaa ttaaatcatc atttccacct tcgaaattta cgagtttatt
     1801 ttgtctagtt ctaccagcaa acttattttc attattttta cttcgtcctt caactagaac
     1861 ttcaactatt ttatctttat atccatcatt tatttctgca gaaattctat ttacagcttc
     1921 taacacccta ttaaatctat catgctttat atcttctggt atttgatttt ccattttagc
     1981 agcaggagtt ccctgtcttt tagaataaat aaatgtaaaa gctgaatcat atctaacttt
     2041 ttcaaccaca tctaaagtgt ccaataaatc ttcttctgtt tctcctggaa aaccaatcat
     2101 taagtctgta gaaaaagcaa tacctggaac ttccttttta gctttttcta ttatttccaa
     2161 ataatattcc tttgtataat gtctattcat ttttttcaaa aggctactgc ttccacattg
     2221 tattggtaga tgtaaaaact cacacacctt atcacaatct ctaattgcgt atattacttc
     2281 atcagatata tcttttggat gagatgtcat aaatcgtacc ctttcaattc cttctatatc
     2341 attcactttt cttaaaagtt ctgagaatga aactgaattt tcaagtgtct taccataaga
     2401 atctacattt tgtccaagta aagtgatttc tttcgttcca tttgcaacaa gctcttttat
     2461 ctcgttaatt atatcttcag gagttctact tctttctctt cctcttgtgt atgggactat
     2521 acaatatgtg cagaaattat tgcatccata cattatattt acaaatgctt ttaattcaaa
     2581 ttttctatta cttctaagac cttcgattac ttctccatcc acatcccata catctactaa
     2641 cattttgtca gaatttattg attctgtaag taattgcgga aatttatata gattatgtgt
     2701 tccaaatata aggtctacat gtttatattt ttttcttaat tcctctacaa catgtggttg
     2761 ttgcatcata catccacata cagctatttt catatctgga ttttttcctt ttagatgttt
     2821 taattgtcct aagttaccat acacttttaa ctctgcattt tctctaactg cacatgtgtt
     2881 atatattatt aaatcacttt cttcaaccat cattgacatt tggtatccca tctcttctaa
     2941 catcgaacat aatttttctg aatcatgttc attcatttgg cacccaaagg tttgagtaaa
     3001 cacgagtttt ctctttcctg ttctgttgaa gtattcttca ttttgtctgt gtatttgttc
     3061 tatatattta tcttgttctt gtattttctc tataggtatt gttacttgtt ttcttttgct
     3121 cacgtctatt cctcctagtt aatttcttat attacacata accttaatta tatcaaataa
     3181 gaaaaatctt ttaaagatat tattattttt ctagttgtat tttaatagta aatctaatga
     3241 tttatatata ctatatttct aaaatataaa atcatataat tattataata ataaaaagat
     3301 gttttaaatt aaaacttaaa acatctttcc ttaaataaag tatatgattt actattaaat
     3361 acaattacat ctattcattt tcaattaaat aacaatcatt taatattaaa tattgacctt
     3421 cctcaaccgt tatgtatctt cttcctgaaa aagtatcatt agtaactatt ccatctgcat
     3481 tacctaagct atcatttcta acttcataat atgcatcaga agaatttgac attataatat
     3541 gttctccagc aggaatttct aatccaattt tatacatacc atttccaaac ttaccattct
     3601 tggccttctg ggctttagca tactttgcta atatcattgt tgaatcctca attttaatat
     3661 attgaccatt ctttaaggtg atatatctag tcccactaaa aatatcattt gaaagtatac
     3721 tatcagcatt acctgtgcta tcacttgtaa cttcataata agaagcataa cttcctgagt
     3781 ttgaagttat taagtattca cctgctgaga tatcttttcc aaccttatac attccatctt
     3841 ttatagtctt tgtagtaggc ttaacatttt ttacaacccc agtattaata tctccaattc
     3901 catttgtgat atttaaacac tgttgtacta tatttgaatc tatatatcct atagaagtta
     3961 tcttcttagc attttttaaa atgcttttat tgctaccatc attaactaaa accattggac
     4021 catgttttga taaagaagaa cctacaagtg catttgttaa ttcataagct ccagctatat
     4081 aaaactctct cgcatcctta taaaacttat ttattattgc tttattagtt tcaaaacgag
     4141 tactcccccc tagtctagta gatttagtat tgtttactaa agtggtactc attgaagctg
     4201 ttcctcctat tgcataagac tttaaaccac tagttgaaaa aggtatactt tgtccattag
     4261 taagaattat tggtgcttta tctcttgctg ctacagatgc aatacttata gcatctgctt
     4321 ctcctttata tgcattggtt aacataacag tattaacttt ttttatcgaa tttatttctt
     4381 ttgcaacatt ataacttgtt tttattctgt catttccctt aattcttaca actttcattt
     4441 tcttagattt taatgaattt tcgacagatg tactaataga atatgttcct ccaattatat
     4501 aaacttttga tacatttttt aatcttgcat atgtctcagt tggtatagta tttttttcag
     4561 ttaatagtat aggggcattt aaagctcctg ctagtccact tgcacttaat ccatctgcga
     4621 tagagttact agtattaatt aatatagcag ttttatatgc atttttatcc gctattttac
     4681 ctgcgatttc atacttattt tttccttgta ttttttctac agatgatatt gcatttactg
     4741 attgaatatt tgcaaataca aaagaaatac ttaaagtcaa tgctaatatt cttttataga
     4801 atttcataat aaaattgtcc ccctcataat aaatttttaa tatataaata catactctta
     4861 aattggttat gtacttaaca aatttatgta aaattgcaaa ctatattaga ccatgttact
     4921 aataaatatc aatgcaatta ttcatttagc tataattgta tttacattta tattaaaaat
     4981 caactagatt tgtataaata cttataatta tatttttatt cttaatattt tttttattat
     5041 gttattttaa tagatttatt tttctttttt aagc
//
LOCUS       DAEMAE010000241         5006 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEMAE010000241 DAEMAE010000000
VERSION     DAEMAE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.244"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..1897
                     /locus_tag="KPH93_003276"
     CDS             14..1897
                     /locus_tag="KPH93_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG1690048.1"
                     /translation="MSNKNKENITLSNQIKGTTQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIIMNKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYVDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDEWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPALGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKDWFYIKDMSKIDTPVEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVINYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTEEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNVDRFKLAYMFMFVQTGCPCVYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNREIFNFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKDIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2122..2802
                     /gene="pgmB"
                     /locus_tag="KPH93_003277"
     CDS             2122..2802
                     /gene="pgmB"
                     /locus_tag="KPH93_003277"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG1690049.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWKKLAHKIGINIDTKFNESLK
                     GISRMESLDRILEFGNKKDLFSKEEKLKMAEEKNNYYISSINDMTSSDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIANAAKCKNNKPHPDIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGSYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2799..>5006
                     /locus_tag="KPH93_003278"
     CDS             2799..>5006
                     /locus_tag="KPH93_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG1690050.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENTFNTPVFTVG
                     SMMYIKTNGKVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESDLLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYVAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEIEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNKFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWDETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIEN"
BASE COUNT         2069 a          455 c          950 g         1532 t
ORIGIN      
        1 gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta atcaaatcaa
       61 aggaacaact caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatataa taatgaacaa
      301 agaagtagaa actgaattat ttgattattg gatagctaag tgcaagcctt taaataaacg
      361 ttcacgatat ggttttataa tagaaggtca agaagaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      481 tggatatcca tatttaaatt atgtagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat gaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttatttttg
      721 tcctattaca attggaaaaa ccaatcatag atacgataca gtagattata tggaggtaga
      781 ccctgcatta ggagataaag agactttaaa aaaactaata gaagaagcac ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaagac tggttttata taaaagatat
      961 gagtaaaatt gatactccag tagaacagat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaattattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agaggagata aatgctgaag agtttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaatg ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacgggatgt ccttgtgtat actatggtga tgaaattgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga atagagagat
     1621 atttaatttt atgaagaaga ttattaggct tcgtaaagaa aataaagagc ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaaca attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa agatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat ttattttaga
     1921 tttcatgtta aattattata cagtttaaac aaatttttat gaacgataaa aaaatatggt
     1981 ctacaagtga gtgaattgaa agtagatttt aaattaaata ctgagttaga gtagataatt
     2041 ttataaataa tatcttaaat acaagataga aaataagttg attagtagtc taaaaaattc
     2101 ttgtagaaag aggaaataag aatgattgaa gcatttatat ttgatttaga tggtgtaatt
     2161 acagacactg catattatca ctatatggct tggaaaaagc tagcacataa gattggtata
     2221 aatatagata caaaatttaa tgaatcttta aaaggcataa gtaggatgga atcactagat
     2281 agaatattag aatttggaaa caaaaaagat ttattttcaa aagaagaaaa attgaaaatg
     2341 gcagaagaaa aaaataatta ttatatatca tcaataaatg atatgacttc gagtgatatt
     2401 ttgccaggga tagaaagctt gttaattgat gtaaaatcta ataatataaa gataggtcta
     2461 tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt tgggtataag tgacaaattt
     2521 gattttatag ctaatgcagc aaaatgtaaa aacaataaac cacatccaga tatatttctt
     2581 atgtcagcga aagggcttaa tgtaaatcca caaaattgta tagggataga ggatgcaagt
     2641 gcaggtatag acgctataaa ctcagctaat atgttttctg ttggtgttgg aagttatgaa
     2701 aacttaaaaa aagcaaattt agttgtagat tcaacaaatc aattaaagtt tgaatatata
     2761 caagaaaagt ataatgagta tatagttagg agaattatat gaaatgttta tttagtaatg
     2821 tagatgagtg gaagattata caagataaga ttgaaatcaa agaaaataga ttagcagagt
     2881 ccataatgag tataggcaat ggatatatgg gtatgagagg aaactatgag gaaaattata
     2941 gtggagatac acacaagggg tcttatatag ctggagtatg gtttccagat aaaactcgtg
     3001 ttggttggtg gaaaaatggg tatccagaat actttggtaa aattcctaat tcagtcaact
     3061 atataggtgt aagaattttc atagatgatg tagaattaga tttagcaaaa tgtaaagtag
     3121 aagattttca tagagaatta gatatgaaaa atggtatttt aaatcgtagg tttattgtaa
     3181 atataaatga taaaaaattt gaagtgaggg ttactaggtt tttaagtata tctgttaagg
     3241 aactcgcagt aattaaatac agtataaaat cattaaattc taatactaaa ataaaatttg
     3301 taccatattt agattcaaat ataaaaaatg aagattcaaa ctatgaggaa aagttttgga
     3361 atggaatttc ttcaagttct ttagaatata aagggagtat tctttcacga acaaaggaga
     3421 atacatttaa tacaccagta ttcactgtgg gttcaatgat gtatattaag acaaatggca
     3481 aagtagaagc tatatctcat gagagtgatt ataattactg tgaaaattca ataacaatgg
     3541 atgttttaaa aaatgaggtt gcaagtatag aaaaatatgt atctataact tcaactagag
     3601 attataagga aagtgaccta ttaagaaaat gtgaagacat tttaaataga gaactatcta
     3661 aaagctatga tgatatatta aatgaacata caaaattatg gaataaaaga tgggaaagtg
     3721 cagatgtaaa aataaatgga gataaaagta gccaacaagg tataagatat aatttattcc
     3781 aactattttc aacatattat ggagaagatt cccgattaaa tataggtcca aaaggattta
     3841 ctggtgaaaa gtatggagga gctacttatt gggatacaga agcgttttgt gttccagtat
     3901 atctaggtgt agcagataag gaggtaacta gaaatctttt agaatatagg tacaatcagt
     3961 tagaacaggc aaaggataat gcaaaaaaac tagggcttaa aggtgctttg tatccaatgg
     4021 taacatttga tggagtagaa tgtcataatg aatgggagat aacatttgag gaaatccacc
     4081 gaaatggaag tatagtatat gcaatatata actatacaaa ttatacaggt gactataaat
     4141 atataaaaga aaaaggaata gatgtaataa tagaaatagc tagattttgg gcaagtagag
     4201 ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg agtaacaggg ccaaacgagt
     4261 atgaaaacaa tatcaataat aattggtata ctaattacgt agcaaaatgg tgtttagatt
     4321 atgctgtaga aaatatctta cgattagaga aagagtgtaa tgaatgtatt gacagaaata
     4381 atgtgactag ggaagaaatt gagttttgga aaataatatc aaataaaatg tatctgccat
     4441 atgatgagga actacaaata ataattcaaa atgataattt tttagataaa gaatttgtag
     4501 aagtaaagga tttaccaaaa gaaaattttc ctcttaatca aaattggtct tgggataaga
     4561 ttcttaggtc gtgttttata aaacaagcag atgtattaca gggaatttac tattttggaa
     4621 ataaatttac taaagaagag aaaaagagaa attttgattt ctatgaaaaa tatactgttc
     4681 atgaatcttc tttatcttct agtatctatt ctattattgc ctctgaaata gataacttag
     4741 aaaaggctta tgaattatac tcaagaacag ctaggcttga tttagataat tacaataatg
     4801 atacaaatga tggactacac ataacttcaa tgtctggttc gtggctttcc attgtacatg
     4861 gatttgcagg tatgagaacg tgggatgaaa cactatcttt tgaccctaag ttgccaaaag
     4921 agtggaaaaa ttattctttc aatataaatt atagagataa tattattaat gtagatgtat
     4981 gtagtgattt aattaaaata gaaaat
//
LOCUS       DAEMAE010000242         4974 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEMAE010000242 DAEMAE010000000
VERSION     DAEMAE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.245"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(144..1430)
                     /locus_tag="KPH93_003279"
     CDS             complement(144..1430)
                     /locus_tag="KPH93_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1690051.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1553..4651)
                     /locus_tag="KPH93_003280"
     CDS             complement(1553..4651)
                     /locus_tag="KPH93_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1690052.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHVK
                     CYKALQGKDSPCEFCTNSILKEDKYYIWENTNTRTNRHFILKDKLIKWKGKPSRLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQSLTNLDIKELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMASSLKQDDEIIGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSVWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     LTNNILFKHKELADKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSRNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQINLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYKILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYGIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
BASE COUNT         1633 a          870 c          454 g         2017 t
ORIGIN      
        1 tctagcattt ttaataacaa gttctagtat tttatatcaa taatttgttc tagtattttt
       61 cataataagc tttaatactt tatatagtaa tttgttgtag aattttatat aatataatga
      121 tttatacaat tttataaaat atattaaaac agtatttcag taatattttt tggtcttcct
      181 ccaccaatac tagtctcttt tgcacatatt cgacctaaac ctgcatttac tattttattt
      241 aatatacgtc ttgcacttct ttcacttaca tttagatact ctgctaactc ttttgaatca
      301 taaatcttac tttttctatt ctcacttata gcaataattt ttgcaactga ctcacaactc
      361 attccagtct ttttagatat ttctattacc ttttcatcag atagcatcaa agaataattt
      421 aattcatttt cggaagctaa aggtcctctt atgttttcat cttcatctac taaatatata
      481 ccaatttctt ttgaatctat ggagtgtctt aatgctttat gtgcattatt ttcagcctga
      541 tacgcagtta ctccagtacc aattcctata ctaagagaaa agcccatgtc ttttatctct
      601 ctctttagat tgaataactt atcataatta tactcattat cagctacacc tttatgaaca
      661 aatacaacat aatcatttct tccaagtaaa aataacgaac cttgaatgct tcttacatag
      721 tttactacaa gcttatccat atctgatttt tttatcatgt cagaataata gttatctatc
      781 ttctctttat aatctattag gctcaatatt tcgacagcaa tttgagaaaa tcttgccttg
      841 cttagagcgt attcactctt taccttatca taactttctt tgattaattg tatagtagct
      901 tgtaaacgaa atacaggata gcctctttct tttaattcat tatatactgc accaaaacca
      961 gttaacatta tatccgtctt attttcttca aataatttta tatgccattc tacaagttca
     1021 gactctccta cttccaaaga aaatggatta caaaatacac tcgttgcttt aatatcaaat
     1081 tctttaagtg catcttccac aacatatcta tcaactacat ctatactgaa tttatctaat
     1141 tttatatttg cactttttat tgcccaaaat gccttcatta tacttgtccc acttctcggt
     1201 acaaatgaat gtggcttgct tatctcatac ttagatttta taaattcata aactgcacat
     1261 cctgtaaata taatagcatc acattcatct tcacatttag aaataacttc tattgtatct
     1321 actacttttt ctcttacata tattttagtc tttaaatcac tgtctatctg ttttaaatca
     1381 tcctttatct taaacccaga gtctgaagga cctataatac ccactatcat aaacttcgct
     1441 cctaatccat aaatattata ctttttaaaa attatatcct cactattttc tacttctatt
     1501 taattatttt gaactactcc atcctaccta catttagaat ggaataatta gcttattttt
     1561 gataaagtaa cttctcaaaa tcagcacttg gaataggctt acaataataa taaccttgaa
     1621 ttaccacaca atctaaactc actaaaaatt cagcttgctc ttttgtctct acaccttctg
     1681 ccactacatc catatctaaa tcttttgcca tggctatagt gtgctttaaa actatttttt
     1741 ctctgtctgt gattccttct tcattaaaaa aaccacaatc tatctttaat gtatcaatat
     1801 taatattttt taacatattt aatgaagaat atgctgaacc aaagtcatcc atatttaaaa
     1861 caataccatc cattctaagg tcggctatta tcttagtaaa taattttata tttttaataa
     1921 ataaactttc tgtaagctct aattctaaaa aagattttgg tataccatat ttatttatta
     1981 aacttaaaac aatttcttta aaatgtggat tatgcaaatg taatcttgat acattaacag
     2041 agataggaac tggctcttca cctaaatcta tccattttct aatcaaagaa catacttgtt
     2101 cccatacata catgtctaat ctgataataa acccattctt ttcaaatagt gaaataaatt
     2161 taattggagg tattatacct ttttctggat gaacccatct aaccaatgct tctgcaccaa
     2221 caattttatt tgcattaaga ttatacttag gttgtaaata tacatggaat tgcccatttt
     2281 caagtgcgta ttccatttct ctttccatct gtttttcttc aagtgcatca tttcttatag
     2341 tatcatcata aaatatataa ttattgatac tttgtttctt tacttttttg tgagcatatc
     2401 ctgccatttc acacgcattt gtaattggaa tatgattatc caaaatttta taaaccccaa
     2461 aacatggtac taacatatat tcaaattttt ttctatttaa gctattttta atcttataaa
     2521 ttactttatg aatatctcta tcagaataac tctcataaaa aatgcaaaat acatctgcat
     2581 acatacgaca acatatacca ttagaaccaa tatttttttg aatcaactct gcaacatata
     2641 ttaaagcttt atctcctcct ctaactccat aaatatcatt aattaccttg aacttatcta
     2701 tatccatcat tataattgca tagcgcttgt caggatatct tttaagcatt aaatttattt
     2761 gttcacaaaa actttcataa ttgtatagcc ctgttaatgt atccttttgg tttttatgta
     2821 gttgcataag ataatctata ttttttatag caactaaaat atgtgtttgt ttctcaccta
     2881 acttatttct actactaatc ttcaacatat aaattaatgc ccaatagtac tttttatcta
     2941 ttcctaaacg tctatactca aaatttagtt ctaaatcgtc ttgcttgcta ttattaagac
     3001 tatcaagaga ttttagaagg tggttatcat caaataatat tatatcgtca ggatgaatta
     3061 aatatttttt tatgtatgaa aaagtttttt caagattttc ctttatagga aaactaaaaa
     3121 tattgctctc tgattgataa attggagtaa ttaaattcgt atcaatgcaa atagataaaa
     3181 ttatatcata tgtattataa gctaattttt cataagcctt agaatatcct tttatcatta
     3241 aatcatcaaa ataaacctta tcagctaact ctttatgttt aaataaaata ttattcgtca
     3301 aatcagctaa gtcactaatt acttccattt cattatttct aagtttgttc ttattatagt
     3361 acctcttctt atcttggtac attctctcgt cagcaacttt aattagctta tgtatatcaa
     3421 tattatcgtc actccaaaca ctgcccactg ctacactata tatcatctca tccttaaatt
     3481 cttcacttag ccaactcact aattcttcaa atagttgttt tgatatatct tcacataata
     3541 caacaaactc atctccacca atacggtaaa cattgtaaga atgaaagtat ttttttatta
     3601 ttacacatgc tgacacaata gcagagtcgc cattagcatg tccaaagtta tcattaatat
     3661 cttttaatcc atttatgtct acaaatacaa cacctataga tttaactttg cctttttgaa
     3721 aattatctat atacttaata tatttgtttc tattatacgc accagttagg aagtcatagt
     3781 agctcatata atttaattta ttttctactt ttcttttttt catctcagtt aataaaaaat
     3841 aagataggga tgtcaaaaga gatgtatctc caacagcctt atgtggatta tcaactccaa
     3901 taaaacctat tatttcatca tcttgtttta gtgaagatgc cattaaactt tcaatttttt
     3961 gaggttttaa aatttcatat tctattggct gttcttcttt tatattttct atatcatcaa
     4021 ttattacaaa gcctttttgt tcgaataata aaaaccaatt ctttaattcc ttaatatcaa
     4081 gatttgttag tgactgtttt tgttcatcaa caccttcatt acaccattca tatgtattag
     4141 ttactttttt tctatcttct gatacttcaa aaacatatgc tctatcagct ttatgaaatt
     4201 taccaatatt ggttaataca agatttacag catcagaaaa attagattct gttataagat
     4261 gtttaataca tccaactaat aattccttta tctctaattt ttctgctatt gacttactta
     4321 tatcttcctt ttctgtaata tcaagagcta tttctaatcg tgaaggttta cccttccatt
     4381 taattaactt gtcctttaat ataaagtgtc tatttgttcg agtattcgta ttttcccata
     4441 tgtaatattt atcttctttt aaaatagagt ttgtacaaaa ttcgcaggga gaatctttcc
     4501 cctgtaatgc tttataacat tttacatggg atatatcatt aagtcccatt gcctttttcc
     4561 caatttcatt aatatataaa agttcatatg tatctaaaga agaaacatat acaatttcat
     4621 ccaactcatc tattaaattt attacatcca tttatatata tctcctttta ttcattttcc
     4681 agattgaatt tttaaagttt ttaaaatttt gctatatatt tggcaatgat attatgtttc
     4741 tttatgtttt aaaatagcac cttaatgagg ttctattttt tagtaaatag aattattatt
     4801 gtgtaaatcg tataatatag ttttgtaata cttaatttaa tatatttata attaattatc
     4861 ttattatgta tttacaatta attatcatat aaatgacaaa tctccacctc aattaagtat
     4921 tccaatcata tataaggata atacttcatt catttaaaaa tataaaacta ttat
//
LOCUS       DAEMAE010000243         4958 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEMAE010000243 DAEMAE010000000
VERSION     DAEMAE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.246"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            609..4748
                     /locus_tag="KPH93_003281"
     CDS             609..4748
                     /locus_tag="KPH93_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG1690053.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANVPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQATPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDEVSVPGVDPNTGFPLPDIGGGTGAEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDIPASIINPEYSLDGGVTFQPWTGNLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPSSIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSPTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGASFQPWN
                     GSLNIGTVVPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVMDSSTVTTPIVASA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLADDIPNVILNPEYSLDGGVT
                     FQPWNGNLNIGTLDTGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPLAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIKNIIIRGTVSQTAIGIIINTATISSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQAVVVPGDVLDYTIEARNAGPSTAQNVTLTDNIPVS
                     ILSPEYSIDNGVTFQPWTGSLNIGTLDTGGIRNIIIRGIVSETAVGTIINTATVSSTT
                     PDPNPNNNTSTSEIDISSSADISVIKLANKTEACVGDEIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKTVFSLDRGVSFQPWTGSLNIGTLTAGTLRVIILRGIIKSTCLDKLTNI
                     AEVTSTTPDSDLNNNISRVQVEIKQCCCNDCCCNNCSNCWCNCCCKDCYCNCCKNDFW
                     C"
BASE COUNT         1905 a          719 c          834 g         1500 t
ORIGIN      
        1 tttagattaa agtgccagat tatgcattta agtagatttt aagcttgatt tgattagaaa
       61 ggtacaaact atcattgaaa aaaattaaac ccatttaaaa attaatataa aagcttgtta
      121 ttttgagaaa aatagactct ctaaacttaa ttaaataaaa ataaaataca gaaaattata
      181 tctctgaata tcttaagtaa ttgagtttaa attttatgat attttgtttg atgatttata
      241 atataaaaac atattcttaa tctgctcaaa ttaaaaagag taaaaaataa caaaaaaatc
      301 actgacattt cactgacaaa ttgtaatttt gttattataa atacattgaa aatacttgat
      361 tattgaaaaa tggcataaaa taataccttt tatgccattt actattattt gataattttt
      421 aacttatgac ataagatatt ttttcacata tacaataaaa tataaaaaag ttgatattgc
      481 aaataaaaaa atacatgtta gatgttatag aaaaaaaacg cacttatagg cttaaaatgt
      541 cacataaaag agttttaaag aataatatat tatgaacaca atttttgtaa tcacaaagga
      601 gtgataatat ggcaacagta tcaggacaaa tagtttttga tagaaataga agtgctacga
      661 ttgattctgg agactcagga atagccaatg tcccagttgt attacaagat atagcaactg
      721 gagttagact tgtggttctc acagatgcat caggaaatta tgtttttact aatgttccaa
      781 atggtaacta tagaattgta gaagcttttg gaactacagg aggagtacca acaccaggga
      841 attttgcaac agcgatagta ggtccagttc caattgcaga aatgccacct attagttctg
      901 tgacaaatcc tcctattggt tcaacaaact tagatggtac aacacctaac acactattaa
      961 taacagtagc aggttcagat ataacaaatc aaaatatatt aaatgggcct gtgatttata
     1021 cacctataga gaatatattg gattcttgcg tatctgtatc taatataaac cttattacag
     1081 atgcagataa cggaacattt gggttttttc cgccaggtac accaagtaat acaggacctg
     1141 aaatagaacc ttacccaaat gtgacgccag attttacgta tgttgtacca gacccaacta
     1201 agtttacacc tttagatgga gaatatacag tacaaaatat tatgacaaat gcaatgagta
     1261 atgttatagg agcttggtgg cgtattgctg accatactac aggaaatgaa acaggaagaa
     1321 tgatggtggt aaatggtttt aatcctggag ctgtattttt taaatcaaca gtacaagcta
     1381 cacctaacac taattattta tttagcactt ggattttaaa cctttttaag gtaacaggat
     1441 atccaccacc acagctaggt gtaagaatat ttgaccaaaa taataatata ctatatcaag
     1501 caacattagg agctcaaatt ccagttaata caaacgcacc agaatggaaa gaagtaggta
     1561 ctgtgattaa ttctcaaaat aataatcaac ttacagtaga attttttagt gagggagaaa
     1621 gcgccattgg aaatgactat gctatagatg atgtttcttt aagagaaata gctgtcccaa
     1681 cttttactcc aataaaaaca agtaacacag aaactgctaa tgtaggtgat ataatcacat
     1741 atacagttac attagataat acttgtgata gccctttaac taatgtattt tttagggatt
     1801 taattccaaa tggtttagag tttatacctg gaactgtaac ggttgatgaa gtatctgtac
     1861 caggagtaga ccctaataca ggtttcccac ttccagatat tggaggagga acaggggctg
     1921 aagtaacttt tgatgttgtg gttgaaagta ttcctaaccc aaatcctact aataatatag
     1981 ctactataga ttactcctat acacctgtag aaggcggtat accaaatgat ttttcagtag
     2041 attctaatcc agttcctgtt gaagtaatct cagcagatat tgaagttaca aaattatctg
     2101 aacctactat agtgaatcca ggagaggaat taatatacac tataaaagta gtaaataatg
     2161 gaccattccc atctgagaat gtggttttaa cagatgatat accagcttct attataaatc
     2221 cagagtattc attagatgga ggagtaactt ttcaaccatg gactggaaat ttaaatatag
     2281 ggacattaga agtaggagag acaagagtta ttataattag gggtattgta aatccatcaa
     2341 cagtaggaat tataacaaat acagctgtag tatcttcaac tacagcagac ccaaacctta
     2401 ataataatac atctacaata gagacagaag tgaatttatt agcagatatt ttagtaatga
     2461 aaacagcaga acctaattca gctgttccag gtactttatt gagatatact attcaagtag
     2521 aaaacttagg accagcaaat gctgaaaatg taatattaaa tgatgatatt ccatcttcta
     2581 ttataaatcc agaatattca ttggatggag gagcaagttt tcaaccatgg aatggaagtc
     2641 ttaatatagg aactttaaac tctggaattt cattaacagt tttgattcaa ggaacagtta
     2701 gtttaaattc ttcagaatat atagtaaata cagcaacagt ttcttcacct acacctgacc
     2761 cagatttaag caacaatata tctaccatta ttacaccagt taacccacaa gcaggtattt
     2821 ctattataaa agtagctgac gaggatgtag ctgttccagg agaagaattt gtatatacaa
     2881 ttgaaatttt taatgaagga ccatctaatg ctacgaatgt agttttaaca gatgatatac
     2941 cagatgttat tttaaatcca gaatattcat tggatggagg agcaagtttc caaccatgga
     3001 atggaagtct taatataggt acagttgtac cagggcaatt aataagaatt atcataaagg
     3061 gattagtaag ttcaactgct acaggagata ttactaatac agcagaagta agtgcagatg
     3121 taccagaacc agttatggat tcatcaacag tgacaactcc tatagttgca tcagcagata
     3181 tagaagtaat aaaaactagc aatatggata cagcagttcc gggagaaact tttagttata
     3241 ctataacagt tacaaattta ggaccatctg ctgcacaaag tatcgttttg gcagatgata
     3301 taccaaatgt tattttaaat ccagagtatt cattggatgg aggagtaact ttccaaccat
     3361 ggaatggaaa tcttaatata ggcactttgg atactggaga aataagaagc attataataa
     3421 gaggaacagt gagccagaca gcagtaggta caattataaa tacagcaaca ataagttcac
     3481 ctacacctga cccaaatcca gataataata cttcaacaga tgaaacagat ataagtccat
     3541 tagcagatat ttctgtgata aaaggcaacg aaccacttgc tataccaggg ggaagattta
     3601 tttatggaat tgaaattgca aatgcaggac catcttttgc agagaacgta actttaacag
     3661 ataatatacc agcttctatt ttaaatccag agtactcaat agataatgga gtgactttcc
     3721 aaccatggaa tggaagcctt aatataggta ctttagatgc tggagagata aaaaatatca
     3781 taataagggg aacagtaagt caaacagcaa taggtataat tataaataca gcaacaataa
     3841 gttcaactac acctgaccca aatctaaata ataatacttc aacatcagaa gcagaggtaa
     3901 gttcatcagc agatatttct gtagtaaaac gaagcaatca ggctgttgtt gtaccaggag
     3961 atgtactaga ctatacaatt gaggcgagaa atgcaggacc atctactgct cagaacgtaa
     4021 ctttaacaga taatatacca gtttctattt taagtccaga atattcaata gataatggag
     4081 tgacttttca accatggact ggaagtctta atataggtac tttagatact ggagggataa
     4141 gaaatattat aataagggga atagtaagtg aaacagcagt aggaacaatt ataaatacag
     4201 caacagtaag ttcaactaca cctgacccaa atccaaataa taatacttca acatcagaaa
     4261 tagatataag ttcatcagca gatatttctg taataaaatt agcaaataaa acagaagcat
     4321 gtgtaggaga tgaaatagat tttgtaatag tagtatcaaa tgcagggcca gaaaatgctc
     4381 aaaatgttac tttaatagac aatgtttcag ataagcttaa gaaaacagta ttttcacttg
     4441 ataggggtgt aagtttccaa ccatggactg gaagtcttaa tataggtaca ctaacagctg
     4501 gaactttaag agttattata ttaagaggaa ttataaaatc aacatgtctt gataaactaa
     4561 caaatattgc agaagttact tcaacaacac cagattcaga ccttaacaat aatatatcaa
     4621 gagtgcaagt agaaataaaa caatgttgtt gtaacgattg ctgttgtaac aactgtagta
     4681 attgttggtg taattgttgt tgcaaggatt gttactgtaa ttgttgtaaa aatgactttt
     4741 ggtgttgatt ttataaaata attttgtata ccttaaatta aactgaaata aaaaataaat
     4801 tcacatatga gtaaaacatt ttataaatat taataaattt tctatctttg atatggacca
     4861 cattcgtaat actagttatt aaatgaagtg gtcctattta attcattaaa attatttatt
     4921 ctaattaaat tttaaatgta ttaaataact ttgatttt
//
LOCUS       DAEMAE010000244         4893 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEMAE010000244 DAEMAE010000000
VERSION     DAEMAE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.247"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..242)
                     /locus_tag="KPH93_003282"
     CDS             complement(<1..242)
                     /locus_tag="KPH93_003282"
                     /inference="COORDINATES: protein motif:HMM:NF024776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1690054.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSV"
     gene            complement(386..976)
                     /locus_tag="KPH93_003283"
     CDS             complement(386..976)
                     /locus_tag="KPH93_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG1690055.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(979..1854)
                     /gene="dpsA"
                     /locus_tag="KPH93_003284"
     CDS             complement(979..1854)
                     /gene="dpsA"
                     /locus_tag="KPH93_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG1690056.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLNQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKDAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(1930..3321)
                     /locus_tag="KPH93_003285"
     CDS             complement(1930..3321)
                     /locus_tag="KPH93_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG1690057.1"
                     /translation="MNYYELLRIKKERHKNKKFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNDEVVKKVNFSIFNPKFNIYMYQYKDLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRKGKIFVNT
                     EYAVYNATKPYSVNDIGYFDNDGYLIFEGRSDDIVNIGGFKVSSTKIENEVKKIPQIE
                     DAVVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3325..4461)
                     /locus_tag="KPH93_003286"
     CDS             complement(3325..4461)
                     /locus_tag="KPH93_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG1690058.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4493..>4893)
                     /locus_tag="KPH93_003287"
     CDS             complement(4493..>4893)
                     /locus_tag="KPH93_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG1690059.1"
                     /translation="LILRPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFY
                     FGFLFAALIISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAA
                     VVPFLIGDTIKSVIASFLGTKLNNVLRRNL"
BASE COUNT         1608 a          851 c          564 g         1870 t
ORIGIN      
        1 tcaacagatt taaattttct attatcattt tctagactaa tatttttaaa tctgccccta
       61 gtttcatcat caaatgcagt tttaccttta tttttataat cttttaccca gttttgaact
      121 tgtgttttgc ttcttatatt cagcatctca gcaattttgt atgatgagta tccttctttt
      181 atatataagt ttacagcttt tagcttaagt tctttgctat attttttgtg ttttttagac
      241 atagaaaaac ccccttagag tatacgcttt ttagacctat tataatagcc ttttatttaa
      301 atgtctacta taaggggatt atactaattt tgagacagcc tctttgctag tattgcatct
      361 aatacataat attatagaac caattctatt gatgtggact ttttataaca ggctgtagct
      421 gtttattctc tagagcaagt tctatagttg gaattattag ctcagtatgt gcaatcatag
      481 aattaggctt tgctttgcaa ttatcttgac caaaaggtac aaaatatata tttttagaat
      541 taaggagaat accaatattt ttgaaattaa agcttagagc atcattcgtt gatattgata
      601 ttactagtgg tttatcatta cgaagatgac cttttgctgc cattaatact ggtgaatctg
      661 taataccatt tgctaatttt gcagcagtat ttccagtaca aggagctatt attataatat
      721 cagcgattcc tttaggtcca aatggctctg cctcttcaat cgtaactata ggtttatttc
      781 ctgtaagttc atatgctttt ttcataaact cttcagaatt tccaaatcta caatcgatat
      841 tttgtgatac atctgaaaat atagtgtgta cattagctcc ttctttaacc aaattctcta
      901 actctataaa tattttatca taagtacaaa acgaaccagt aaatcctact ccaattgtaa
      961 gtccttcaag tctcatttct aatcccttct ttcatttata atattttcta tagcgttaac
     1021 aagaatttca ccagatgtct ttggtgaata tttaccagga agcccaaggc aaagagtcgc
     1081 attaatccct gcgtctttaa caacagaata atctactcct ccaggagaag aagctatatc
     1141 gataatagtt acttcttgtg atactttttc taatctttct ttcgttaaaa tacactctgg
     1201 tatggtgtta aaaatataat caaattgtaa aagtttttca tcaagttgat ttaaagaaat
     1261 actggaatat ccaaatgcat tagccgtact tcttgcatca ttatttcttg caccaacaca
     1321 tacatttgca tccaaagcgt ataatttttt tgcaagtatt tgtgcacatc ttccataacc
     1381 taatactaaa cagttacttt gatgtatatt gattatactt cttttaatag cttctgctat
     1441 agccccttca gctgtagcaa ctgcatttaa aatagcaaca tcattcattt tcataaaatc
     1501 ataaatctga atagagtgtt gctcgcaaaa tttacaaact ttagttggta tgttacctcc
     1561 aaataatatt tgattaggag ataaattaat tagcaagtta tcaatagtag catcaagaca
     1621 atcatcagta ttttcaatat taacttgatt ctttgtaaat ggaattggac ataaaataat
     1681 ggaacttttg gatagtgctt cagctagtga agttgatttt ccaacgattc catttataat
     1741 ttcatttgat atcccataag tgactacaga atacccttta ttaattagtt tttttaccat
     1801 gtacacttgt cgcaggtctc ccccaataac agttatatcg tattgatttg acatattgta
     1861 agcctccttt actatataca atatgttttt atcaatacat tggttcacta aaattactaa
     1921 atattatact tataacattt ttaataatgc taatctatct atcttttcag aagaggtata
     1981 tggaaatgaa tttaaaaaga taattttctt tgggatttca tttttcatta gattatcttt
     2041 cattttaatc aataaatctt tctttgtaat tttgtctata gttattacaa ataatacaat
     2101 ttgatttcct cttatagtgt ctgaatacgg caatacaact gcatcttcaa tttgaggaat
     2161 ttttttaact tcattttcaa tcttagtaga actaacttta aatcctccaa tatttacaat
     2221 gtcatcactt cttccttcaa atattaaata tccatcatta tcaaaatatc caatatcatt
     2281 gacagaataa ggtttagttg cgttatatac tgcatattct gtattcacaa aaatttttcc
     2341 ttttctaatg tatatatcta ttcctggaaa tggtctgcct acacttagag gtttttcaat
     2401 tagttcatca tagcataaat atgtaatata atttaattca cttgccccat agtataaaat
     2461 aatttcagac tttggcatat atttttttag atttcttgca gtatcttcaa ataataattg
     2521 agaccctgta aaaatactta caactttgag tactggttct tttaagtgtt taaccaaaag
     2581 ttgaagtttt gtaggtataa ggtatatgtt agttacatta tattgtttta ttgtcctgat
     2641 ccatgattta caattaagac ctgaacttat tacaatacta cctccttcat ataatactga
     2701 cattatagaa tttagattcc ctgtaaaact aagtgttcca ttaacaaaca agattgattc
     2761 tccagagata ttaaaaatgc cattttgtat aggaaaaaat ccagcccaac tctcatatgt
     2821 tctatataat acttttggaa cacttgtaga acctgaagat aaagctccca tacaaatact
     2881 tttatctaaa taatttataa gatccttata ttgatacata tatatattga atttaggatt
     2941 aaaaatagaa aaattaactt tttttactac ctcatcattg ttatcatcat ataaatcaat
     3001 aacgtccata gattcatcag atataatgat tgaaatatta ttttttagta aaatatcatc
     3061 taatactttt ttagaaagat tataatgaca aataatagga atattttttg aataattaat
     3121 agcaaaaaaa ctaatcaact gaaacattat atttttagaa tatattaaaa ttgggatatt
     3181 ttcacctaaa gaaatttgtt ttcctagttc ctcactataa ctcaatatat tttcataagt
     3241 atatttttct ccatctataa ttaaaaattt tttattttta tgcctctctt tttttattct
     3301 taaaagttca taatagttca taatctacac cctttcaagc agtaaagctg aacctagacc
     3361 accagctgca gctatagagc aaattccata tctaccctta gtcttttcta atgcttttaa
     3421 aagatgtaat gcaataattg cacctgaagc accatatgga tgtccatatg ctaaagctcc
     3481 accaaatata ttatatctat ctattaactc tggatacttt ctttgaaaca acacatctat
     3541 aactgcaaat gcttcattaa attcaacagc agatacatct aaataattaa ggctttctat
     3601 ctctaaaagt ttatccattg cttgtatcgc tgatgttgga cttaaggttg catctgttcc
     3661 tattgtgcat gtattaataa tttttgcttt aggcttcttt tttacatgtt ctaaatactt
     3721 cttagaacat attataataa atgaagctcc atcattaata agacatgaat ttgctgcatt
     3781 tgtaatagtt tcttttccta aaattgatgg catcctatct aataaccttt gactcatctt
     3841 atcacgtata ccttcatcat aggtactatt atttatagga gatataatat cctcaagtat
     3901 tttttcttct cttgcttctt ttgctctttt atgactttcc tttacccaaa aatccaagtc
     3961 tgctttctca atttgatata actctgctac tctttccgcc ccttcgagca tactattttg
     4021 gctattatca tcaggagaaa attgagcaac tgtataattt ggattatttg tattatatcg
     4081 cttatcattc ttatgatatg tgcgcattgg ttgtagagaa ctactttcaa aaccacctgc
     4141 aataattaaa tcacactgac cagatttaac ctttgaaaaa gctatgtcta tactcatcat
     4201 agcagaagca cactgcatat ccacagtaaa agcaggtact tcatttgaca ctcctgctgt
     4261 taaagtcatt aatctagcta tattccctcc tgtcccaact gcatttccac atataatctc
     4321 atcaattttg tcaatttcat actttttaat taaatctttt aaaacacttg ctcctagtaa
     4381 ttcaggttga acaaattgaa aaattccatt ttttagacct atatgacttc tcaatcctcc
     4441 taatataaaa acctccttca tacattacac ccttccttat attatagatt tatcatagat
     4501 ttcttctgag tacattgttc aatttagtac ctaaaaaaga tgcaataact gactttattg
     4561 tatctccaat taaaaatggt actactgctg caaaaagtgc tgcttctact gtcatttgat
     4621 taaaatagca cataaatact gttcccataa aatatataat aggcattcca acaaaaattg
     4681 ttattagtat atatctctta atatcattat ttcgtccttt taataaacta ataattagtg
     4741 ctgcaaacaa aaaaccaaaa taaaaaccac ctgttgggct taacaacttt ccaataccag
     4801 atgtacctcc accaaatact ggaatcccaa ttaaacctat cattatatat acaccaactg
     4861 tactaaatgc ttgtcttggt cttaaaatca aag
//
LOCUS       DAEMAE010000245         4867 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEMAE010000245 DAEMAE010000000
VERSION     DAEMAE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.248"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            344..3178
                     /locus_tag="KPH93_003288"
     CDS             344..3178
                     /locus_tag="KPH93_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1690060.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIQSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIRVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSIL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDEDLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            3221..4465
                     /locus_tag="KPH93_003289"
     CDS             3221..4465
                     /locus_tag="KPH93_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1690061.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMLD
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGNFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         1906 a          623 c          714 g         1624 t
ORIGIN      
        1 ataaattatt cttaacttta tcattttttt catatatctt catctatatc gaccatatat
       61 taattacgtt gaccatatat taataaatga tattacatag actgaagtat attaaaaaac
      121 ttaaatttag ttatttaaca acataatact ctaatttaat ctataaaaat ccatatctaa
      181 tataatgcaa ctcttatttt aacaccatct attacacaaa acaacatatt aaaaagtaaa
      241 atacaaaata tccactataa accttatttt aatactaaat atatattata atatatttaa
      301 tgtagcttaa atatagttta aacataaaga aaggaaacgg tatatgctaa gaaaagataa
      361 agttctatta tctctacata aattatgtga aaatatgact ttaaaagacc ttcaatcaga
      421 taaaacagga ttcactacaa tagaaattgc taaccattca tctcttgata gaagtaatac
      481 cagtaaagaa ttaaataacc tatataatga aaaaaaagtt ctgaaaatat ctggtaagcc
      541 aattttattt ctacatgcta gtattataga aaatttaatt ggccgtactt tgaacagtga
      601 tgaattttct ataaataatt gtaacgagct acttaaatca agtaaaaatg actcagaaga
      661 tattttttca acattacttg gacatgactc tagtcttaaa ctacctatag aaaaagctaa
      721 atctgctata ctctatcctc caaaaggtct taatacatta ctcataggac ctactggtgt
      781 tggaaaaagt acatttgccg aaacaatgta caaatatgct gtagaaagta aaatcttttc
      841 ttctgaaagc aaattcgtag tatttaactg tgctgaatat gcagaaaatg ctaacttatt
      901 attaagtaat ttgtttggtt atgtcaaagg ttcttttact ggtgctaata aggataaatt
      961 cggcatagtt gcacaggctg atggaggcat attattctta gatgaaatac acagattacc
     1021 accagaaggt caagagatgc tttttctttt gatggacaaa ggcatataca gacgattagg
     1081 agaatctgac attgtgcaca aagctaatgt ctttattgtg tgtgccacaa cagaagatat
     1141 acagtcttct ctacttggga cttttttaag aagaatccct gttacaatta acctacctgc
     1201 tttacaggat aggtccttaa aagaaagatt atctcttatt aaatacttct ttattataga
     1261 atcaaaatac accaatgcaa ctataagagt tcacaaagat gtaattaaat cattactcct
     1321 ttatgattgt tttggtaata taggtcagct taaatctgac atacaactta tgagtgcaag
     1381 agctttttta aattacaaga ctggacttaa ggataatata gaaatagatt tatcccttgt
     1441 tcctgattac atctatattg gccttttaaa attgaatgaa aatcgtagta atttaaataa
     1501 attatcagaa cttgattata tgctttttta tgaatttaat ggtaataact tagaagttga
     1561 atatacaaaa gaactctcta aatacaatat agatacatca aacaatggat ttaaatactc
     1621 tacagagatt ccttatctgg aaaatgatat aaggagtatg tttataaatt acatggaaaa
     1681 gtactctaca aaattacttt cgatatcttc agtaactcat tctgattata caagtaatga
     1741 aatttttaaa gtaatcaatc caaagattta ctatgctgtt gaacattctt taaaagaggc
     1801 tgaaaaaata tcgagaaaca aatacacaaa acaaacttat attgctcttt caatgcatat
     1861 aagtgctctt atagaaaata taaatcaaga cacatataat cctaagtaca atttatataa
     1921 tgataaaatt ttcttagata aagatttaaa tatagctaaa atagttttaa gtataattga
     1981 aaacgactta caaacatctt tacctaaaga tgaagtaaaa tttatatcca tgtttctaaa
     2041 tagcatgtat cttgaaccaa acaatcaatc tgaaagtata ggagtcataa tacttgctca
     2101 tggaaattca acagcaagca gtatgtgtga tgtagcaaat tcattgcttg gtacaaacca
     2161 ttgtaatgca attgacatgc ctcttgatgt aaaggtaagt accatacttg ataaaactat
     2221 agaattggta aaagaatgtg acgaaggtaa gggtgtactt cttttaacag atatgggctc
     2281 tcttatagct tttggagaac ttatttcaga aaaaacaaaa atcaaaactc gctcaattga
     2341 aatggtatct actccaatag ttttagaagc agtaagaaaa agtatactac cagaaatgac
     2401 tttggatgaa ttagctaact ccttaagctc tttaaatcca tatattggac gagcagcaat
     2461 tgattcaaca gaaataaact atcctgaaaa gtcaagagag tatgtaataa taactacttg
     2521 tataacagga tatggttcag ctatgaaagt agcagacttt ataaattctt cacttaagaa
     2581 tattagtaaa tacaatatta atttagttcc tcataatata gaaagtttta aaatgtataa
     2641 gaataaattt agtaatacta aaattctggc agttgttgga agtgtagatt ttaatgtagc
     2701 caacacccct tttatacaca tagaagaact actaaatgga tatggcctag atatgctaaa
     2761 taaaataata gaaggtacat tcaatgccaa tagtattaca aatataaaag aaacaaatct
     2821 atcatacaac agtttaataa taaactttat gacccaaaac ttagaacttt tggactctgt
     2881 taaattattc aactatgtca attcttcctt atttacaata atagaattaa tagatgaaga
     2941 cttaaagagc aaatttaagc taggatatat actacattgc tcttttatga tagaaagatg
     3001 ttttaaaaat acaacttttc catataataa cattactgaa ttaattagta aaaatcatga
     3061 actatatagt ttaatacgtg aagcctttaa accaacagaa gcttatttta atattctgat
     3121 accagacgaa gaaattgctt atataataga catgattaat gagtatctaa ataaataaaa
     3181 tttatagcac ttattttgtg catattaatt aatatatgaa atggaggacg agattttgaa
     3241 aggaaaaata aaacagttaa caatacttgc attaattttt atatttataa ctcctgtatt
     3301 tgcatttgca gatactccac cagtaccaaa ttcttcaaga gcagcactac ttatagacca
     3361 ggaaactaaa agaatattat ttgaaaaaaa cattgatgaa aaaatgccat tagcgagttt
     3421 aagtaaaatg atgacctttt tattggcaat agaagcagtt gataaaaatc aagttaaaga
     3481 gactgatatg gtgaaaatag acaagtctac agcttcagta ggaggttcta cttgtaaact
     3541 taaagatggc gatgaaatat ctctaggaga gcttatgcaa ggtctaatgc tggtatctgg
     3601 aaatgatgct gctatagcca tagctaaaca tattggtaaa actgaaaaga attttgtaaa
     3661 tatgatgaac aagaaagcag aagaaatagg aatgcttgat acgtattatt ttaatccaaa
     3721 tgggcttcct atatatactg acccagagca taaagaacct cctattgaaa atatgtcaac
     3781 tgctcacgat atagttacac ttggaaagta tatgtacgac cattatgaaa atcaagttac
     3841 tagaataacc actatgcaag tatataatga tactaaaaag gattttacac attataacac
     3901 aaatccccta cttgtttcag tacctggtgt tgatggaata aagacaggat atacagataa
     3961 tgctggatat tgtcttgcat tttctatgat ggtacctaaa gatgctaaaa acgaaagaaa
     4021 tcatagattg ataggtgttg tacttggtga tggtaacaag aaaaatagaa tttcttcttc
     4081 tgctacttta ttgaaatatg gtaaagataa tttccattct aaaaagatag cacataaggg
     4141 tgatattata gagactcctt gtgttgatgg tattgatgat tttaagataa cagttaaggt
     4201 tgataaagat ttgtatggtg ttgtttcaga taacgagaat atcaatccta aggtagtatt
     4261 taaaaatatg aattatccta tccataaggg agatattgtt ggtgttgcaa agtattataa
     4321 tgattctggt aattttgttg gaagtgttga tgtaaaaagt gaaagtaata taggatgtat
     4381 tcctttgaag gataagatta agattaaggt tgctaagata aataaaaaac ttgagattaa
     4441 aaattctgtt tgttttaaag catagtaaag catattatta aaagggtatg ttaaatttat
     4501 aaagactcca ctttgtggag tttttttgtt ttttacaaaa aattaaaata gtggcaacaa
     4561 ttgaggatgt gagggagagt tgccactatt ttgtataggg tattatatta tgtttttaag
     4621 ctagttttac tatcttctat gtttatataa taactcttgt gtgtgaattt aattgataat
     4681 taatatgaaa attatgtgaa gttttgtaat aaaaattaaa agtgtagaac ttatttaaaa
     4741 tatatattta aataatacaa gaatatttta aagagtacta agttttatgc aaagtaacta
     4801 agttttatat attatgtttt aaatatctta aatatactag atacattaaa tattagtatt
     4861 cattttc
//
LOCUS       DAEMAE010000246         4864 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEMAE010000246 DAEMAE010000000
VERSION     DAEMAE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.249"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            215..883
                     /locus_tag="KPH93_003290"
     CDS             215..883
                     /locus_tag="KPH93_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690062.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKFKSR
                     KGFRYDRDFIEARREEINIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1042..1548
                     /locus_tag="KPH93_003291"
     CDS             1042..1548
                     /locus_tag="KPH93_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG1690063.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGVTEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1676..2692
                     /locus_tag="KPH93_003292"
     CDS             1676..2692
                     /locus_tag="KPH93_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG1690064.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTSANKKNKSSNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKDAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3046..3750
                     /locus_tag="KPH93_003293"
     CDS             3046..3750
                     /locus_tag="KPH93_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG1690065.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGK
                     CKISILDIDNKEVSVD"
     gene            4336..4815
                     /locus_tag="KPH93_003294"
     CDS             4336..4815
                     /locus_tag="KPH93_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG1690066.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGVVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEHSLFVKENVKRFRLIGYLLLLNSIIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         2043 a          379 c          815 g         1627 t
ORIGIN      
        1 aagtacttat ttgaaatatt tgagtatact ttataagtat ttttatgcta actagaaaca
       61 tttaatttat ggcatttgta tttgatttat tttaatgtga ataatttcaa agttaatttt
      121 attcaatata gtactgtaaa agtataaata acatttaata aaaatatata ttatattagg
      181 tattttaaat aaatttttta aagggtgatt ttttatggaa aatataacta gttattttac
      241 aacgatacta tgtgtattta tatgtttatc atctgtattt atatttactc aactagctag
      301 agtatttatt aataagaaga agattaatca aaaatttaaa tcaagaaaag gatttaggta
      361 tgatagagat tttattgaag ctagaagaga agaaataaat ataaaggata acaataataa
      421 taaaaataag agtaacaata agaaattaaa ggaagaaaaa gtatttaaat acgataatgg
      481 tgatttatat aaaggtgaat ttgttgatgg aaagaaaaat ggatttggaa tatatatttt
      541 ttcaagtaag gaaaagtatg aaggattatg gaaagatgac aagatgcatg gaataggaaa
      601 atatacttat agagatggtt caatatatac aggcgaattt aaatacggat taaaaaatgg
      661 tttgggaaaa ttaacatatc ccaataatga tatttataag ggatattttt tagataataa
      721 gaaaaatggt aagggtgtat tatataaaaa tgatggaaat aaacaagcag gaatatggga
      781 agatgatgaa caatgtaaaa gtttagattt taaagattta aataataata aaataaataa
      841 tgtttatcaa aatagattaa ataacaataa attaaaagtt taattattct aataaaaaac
      901 tctttattta tgatggaata taggatatat ctatgttgta atttgtaaaa tcttcctata
      961 tcatataaaa ttaagtattg attagtctat tacataaata tagaatataa atatatccat
     1021 ttaataataa ggagaagaga tatgaaagag tatgtagttg tggatttaga aactacagga
     1081 ttagatccat ataaaggttg tgaaataata gaaataggag taacagaaat aaaaaatgaa
     1141 caaattgtaa aaaactattc aaggttaata aaacctaagg ggataatttc ttcatttata
     1201 acagaattga cacatataag taatgaaatg gtagaaaatg aagaaccact agaattagtt
     1261 ttacctagat ttagaaaata cataggtgat agaactataa ttgcacacaa tgcgaaattt
     1321 gatttgaaat ttttaaatta ctatcttaga atgttagatt tagaaccaat aaacaattat
     1381 atatgtacag tagagttatt aaaaaaatgt aaaagttata aaggaaaaaa taagaaactt
     1441 gaaacagctt gtgcttatta caacatagag aatataaatg ctcatagagc tgacagtgat
     1501 acactagcaa ctgcaaaact atttttgaaa ataaaagatg aatattaatt ataaatatag
     1561 agaaaattag tgaaaatatc ctttataaaa ttgaccctta aacttacaat tttgttcttt
     1621 gatttatttt gtagaacaat gtataatgta ccttatatga agaagggaga ctaaaatggg
     1681 ttattacaat gataatgata ataataaaat aagacgtaaa aaaattagta atagcaaaga
     1741 tagtaataaa aaatctaaac attcttatac ttcggcaaat aaaaaaaata aaagttctaa
     1801 tacaagttct agtaaaagaa tgagtgaaaa agtagattca cagaatctta taaaaagaga
     1861 tagagatagt tttacaagag ataatgtaag acctttaaat acagattaca atcaaagaga
     1921 aagaagaaga gtcgagtata aacaaagaga gaaaaaatta aaaagtaaaa aaagaaaaaa
     1981 acaaaaaagg attagaatag ttagggcatt gttatctatt atgttagttg cactaattat
     2041 attttcagtg gcaggtgcaa tatttacagt ttcagctata aaagggtcac cagaagttac
     2101 taaaaagcta attaggtcta aatatataag tagtgaggtt gtaagtataa aacaaatgcc
     2161 aaatgattta aaacatgcaa tagtttctat agaagatgag agattttata agcataaagg
     2221 agtagatgtt atttctctag ttaggtcagt attaaacaat gtgtttacag atacaacaca
     2281 aggtgcaagt actatagata tgcaagtttc aaaaaacctg cttactagta atgataaaac
     2341 tatgaagcgg aaaataaggg atatgtataa tgctaagcag atgaataaga ttatgactaa
     2401 ggatgaaatt cttgaagcat atttaaataa tatgtatcta ggtaaagatg catatggagc
     2461 tgcaaaggga gctgaagtct actttggtaa aaaagttagt gaattaaatt tagctgagtg
     2521 tgctatgtta gctggtatga caaataatcc tgcaaggtat attgaccatg gagaggctaa
     2581 gaagagacaa gtgattgtat tgtataagat gcatcagctt ggttatataa ctgataagga
     2641 gtatagaata gcaaaagctg atgacactcc atttaaatca gaaattgatt aaaattgtaa
     2701 gaatatagta ttttcaatag tttaaacaaa tgaaaaggta ataaaaaagg tgatttgcca
     2761 cccatttgcc atcataatta taatctaatg gcaaattgtt ttaatgtaca aatcagataa
     2821 tataatattg ttttaaatac tagattatgc taaaatttaa ttgtattttg taaattaaaa
     2881 aatttataac ttagttttct agggttccgc attttataat ggtcaggtcc aagagaaaac
     2941 gcacaattat tgtgtacacg gaaggataaa agcctgggag atatttaata atatttctat
     3001 aggctttttt atttttacat attttaaatt tgaggaggtt ttctaatgaa aaaatttgtt
     3061 attgaggatg cattttggaa tctatttcct agtgcaaaaa ttggagttgt tatttgtaat
     3121 gatatagata actctataaa agatgaagac tataagaaaa taattttgaa gggagaagaa
     3181 gaagcattaa aatacttgga ggactcagaa tttagtaaca accaagtgat taaagtatgg
     3241 agagaagcat ttaaaaaatt taaaacgaaa aaaggagcaa ggtcttctat tgaggcatta
     3301 ttaaaaagag ttcataatgg aaataatata ggaactatta atcctttagt ggatatctac
     3361 aattttatct cattaaaata tgcattacct tgtggagggg aggatataga taaatttata
     3421 ggagatatta ggctaactag agcaattggt aatgaggagt ttatcccact aggtacagat
     3481 gaaaattcat ttccatatga aggagaaata atttataaag atgatggtgg agcaatttgt
     3541 agatgttgga attggcgtga agcagtgaga acaatgctta ctgaagatac taacaatgcc
     3601 tttttatgta ttgaattagt agatgaaagt agatttaaag aatttgaaaa tgctttaaat
     3661 gaattagcta aaatagttca agaaaaattg ggtgggaagt gtaaaatttc aattttagat
     3721 attgataata aagaagtatc agtagattaa tctggaggtg ctgttgtaat agattatttt
     3781 agtcaaaaag atacttatat tgaggatagg aaaatttatt ttagatataa caattattaa
     3841 agtatataat gaggattagt attctattga taagtgataa gataaaaaat attgtcaact
     3901 aattatcttt agataaaata attgttaaaa gaacaaatta tgaaaagttt aagaaaaaac
     3961 ttggtatacc taatgaaaga tttgaagaaa tttcaagagg attatataat attaaaagaa
     4021 tttaaaaaca aaaataacta agtattgata tgattagtta tgaatatgat tatatttaat
     4081 attataaggg aaagaaagtt ataagtttaa aatgatttat aagtcattat atcttataaa
     4141 tataaaataa agatttagaa tacaacaaaa gcctttagaa tagaaagaaa cgtattttaa
     4201 gggcttttta ttatactctt ttttaattac ataattaaca ataaaaatat attgttaatt
     4261 atgatgtttt tattgaaaaa cgatataaga tgatgtatta tactatatat aaattatttt
     4321 aaaggagttg gtatgatgtc taataaactt ataaaaaatt tactaagtgg tatattacaa
     4381 attttatttt ttcttttagg agtagtaatt gttgcaggag gatttaaaag ttttatgtac
     4441 ctttgtttta gtggagaagc aactctacaa ggtactattt atggaatatt aatgtttatt
     4501 ttaggtgtat catattttat tattataaaa tcattgatag aagtattagg tagttctgaa
     4561 catagcttat ttgtaaaaga gaatgtaaaa agatttagac ttattggata tcttttgctt
     4621 ttaaactcta taattgagtt tatttccact tttggtacta ctggaaaagg aatgagattc
     4681 ctagatttag gatttggatt ttattttact gttccagtat ttgtatattt tataacttct
     4741 cttatgagtt ttgtaattgc agatggattt gtaaaggcaa taaagataaa agaggataat
     4801 gatttaacta tttaggcgtg acattttaat atagtaggag ataaataatc tgaaaagagt
     4861 gatt
//
LOCUS       DAEMAE010000247         4811 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEMAE010000247 DAEMAE010000000
VERSION     DAEMAE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.250"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    233..464
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            572..2053
                     /locus_tag="KPH93_003295"
     CDS             572..2053
                     /locus_tag="KPH93_003295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG1690067.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSKINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFHGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKVDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2063..2956
                     /locus_tag="KPH93_003296"
     CDS             2063..2956
                     /locus_tag="KPH93_003296"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG1690068.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSAGVFNVSRVALLVSALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3080..4141
                     /locus_tag="KPH93_003297"
     CDS             3080..4141
                     /locus_tag="KPH93_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG1690069.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4283..4795)
                     /locus_tag="KPH93_003298"
     CDS             complement(4283..4795)
                     /locus_tag="KPH93_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG1690070.1"
                     /translation="MSYFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLIKED"
BASE COUNT         1783 a          602 c          761 g         1665 t
ORIGIN      
        1 gtaatattac agatatttaa tggtcatctt ttgattactt tcattaaaaa caccttcatc
       61 actatgaaaa tctaggtttt taagctttta aaaaagttaa aaaaatattt ttctaatact
      121 caatttgatt acaatttttt tacaatttaa aaaatatttt ttccaaattt ttatattaat
      181 acttgacttt gtctgtttta taaactagaa taattaataa tattaagaat attgaaaggc
      241 attgattagg aagagtaaat ctgaatcgat taccagagaa ggaaactact agctgaaatg
      301 tttccctaat tagaaggatt gaaaactacc taggagcttc tatccgaagt tatcttaatg
      361 attttagtag gaatagacgt ttttattact tgcgttaaag tatcaagtgg attttttaaa
      421 atcaatttgg gtggaaccgc gggaaacaga ttctcgtccc ttttttaggg atgagggtct
      481 ttttttatta cctaaattta atttttaaaa tattctgtat ctaacttaat tagctaatta
      541 aaaatttaaa atatatggag gattataaaa aatgtattat tcaagtacga gaggcaccga
      601 agaaaaagta actgcatcac aagcaataat aaaaggaata tcaagtgatg gtggtcttta
      661 tgttccaagt gaatttccca atgtaaaaaa tgaacttata aatttagtaa atttaacgta
      721 ttctcagata gcattttttg tacttagtaa atttttatgt gattttactg aagatgaaat
      781 taaaaactgt atagaaaatg catatgatga aaagtttgat tgtagtagta tagctccatt
      841 aaataaagta aatgatactt atttcttaga actatatcat ggtccaactt tagcatttaa
      901 agatatggca cttactataa tgcctcatct tcttaaaaca tctataaaga aagataattt
      961 agagaaagat gttgtgattc ttactgctac atctggagat acaggcaaag ctgcacttga
     1021 aggatttaaa gatatagata aaataaagat aattgtattc ttcccagaag atggtgtaag
     1081 cccagtacaa aaacttcaaa tgaaaactca aactggaaaa aatacatatg tagttggtat
     1141 aaaaggaaat tttgatgatg cacaatcagg tgtaaagaag attttttctg ataaagaatt
     1201 taatcaaaaa ctattggata ataattatat attatcttct gcaaattcaa ttaatatagg
     1261 aagacttctt ccacaagttg catactattt ttattcatat atgaaattag ttagcaatag
     1321 taagataaat ttaaatgata aaataaattt tgttgtccca actggtaatt ttggtaatat
     1381 tctagctgga tattatgcca aacaaatggg tcttccaata aacaaattga tatgtgcatc
     1441 aaatgataat aatgttcttt atgatttctt taaaactggt gtatatgata aaaatagaac
     1501 attgaaatta actacttctc cttctatgga tatattaatt tcaagtaatt tagaacgatt
     1561 actgtttgaa atatcaaata gaaatactga tattgtaaat aagctgttat cagaccttag
     1621 tgataaagga gtctacaaaa taagtgatga tatggaaaaa aatttagcct cattccatgg
     1681 agaatattca aatgaagaag aagttaaaag cacgataaac aatgtgttta aaaattataa
     1741 ttatttgata gatactcata ctgctgttgc atacaattgt tatgaaaaat ataaaaaaga
     1801 aacttctgat aacacaaaaa ctgttattgt aagtactgca agtccattca agttttctga
     1861 agatgttcta aagtctatag attgtgattt taaagacctt gatgactttg ctataattga
     1921 tagattatca agtatttcta aagtagatat tccaaaacct attaaaaatc ttaaaaatgc
     1981 agaaatacta cataaagata tttatgaaaa agatgaatta aaactagcta taaaaaaatt
     2041 cttaaaggta taggtgatta ttatgttaga gattatagtt cctgcaacaa gtgctaatat
     2101 aggtccagga tttgattgtc ttggaattgc gttaaatata tacaataaat tttatgttga
     2161 agaaattgaa agtggtcttg aaatagaagg ttgtgaagat gcttacaaaa acgaaaataa
     2221 cttagtttat acatctatga agtacttttt tgatagagtt aaaccagaaa aaatacctgc
     2281 tggtatcaaa ataaaaatcc aaagtgaagt acctatatgt agaggtcttg gcagtagtgc
     2341 ctcttgtata gtagctggag ttatagctgc aaatgctcta tcaggtgcta atttagataa
     2401 aaatcaatta ctaaatatag cttcagaaat agaaggtcac ccagataatg ttgctcctgc
     2461 tattttagga aatatgattg tatctgttac tgataatgag aatatacatt atgatattat
     2521 aaaaattcct gaagaactta aattttgtgc aatgatacct aattttaagt tatcaacaga
     2581 aaaagctaga ggtgttttac caaaggaaat accatattct gctggggtat tcaatgttag
     2641 tagagttgct ttactagtat ctgccttact aaacaaaaac tttgacctac ttaaagtagc
     2701 ttgtcaagac aaattgcatc aagattatag gggaactcta attgagaact acaatgatat
     2761 tgttgaaaag tctgaacaac taaatagtat tggagtattt ttaagtggag ctggacctac
     2821 tataatgtct ttaataaaag aaaatgatga tagttttgta gataatatga aaaattacct
     2881 acaaaaacta aaatctgatt gggaaataaa agagttatgt tgcgattcta atggagctgt
     2941 tttaaatata atataattat cgtcttctat acaaaatata actactcttt tattataatt
     3001 tgtagtataa taattaggac taggtaataa tattaagtat tataatctag ttctttaaac
     3061 atgtttacat tgaggaggga ttatcataaa agagttaata attagagatt tagttgcaaa
     3121 agttcctata attcaaggtg gaatgggcgt tggtatatct aggtcatcac tagctggtgc
     3181 agttgctaag cttggtggtg ttggtgtaat ttctggagtt caaattggat atgatgagga
     3241 agattttgaa actaatacta taaatgcaaa tttaagagct ataaaaaaac atatttctaa
     3301 ggccaaagaa atctcaaatg gtggtattat tggtattaat tttatggtag ctatgaaaga
     3361 atatgaaaca tacgtaaagg aagctgttaa agctggtgta gaccttataa tatctggagc
     3421 tggattacct aataagctac catcattagt aaagggaagt aatgtaaaaa tagcacccat
     3481 agtttcaact gcaaaagctg ctaatgtaat tttaaaaatg tgggatagaa aagagaaaac
     3541 tactgctgac ttaatagttg ttgaaggtcc aaaagctggt ggtcaccttg gatactctaa
     3601 cgaggaactt gataacatag attctataga ctatgataag gaatttgttg aaattttaaa
     3661 agttgcaaat acttatggag aaaaatttgg tagaaacata ccagtagttg ctgctggtgg
     3721 tataacttct agtagtgatg ttaaaaaata tatagatatg ggtgcaagtg gtgtacaagt
     3781 tggtacaaga tttgttgcta cttacgaatg tgatgcccat gagaacttta aaatggcata
     3841 cataaatgct agtgaagaag atgttcatat agttaaaagt cctgtaggtc ttcctggacg
     3901 tgccataaga aacaaattta tagaagaagt taaaataaat cgtccagaaa taaagaagtg
     3961 ctataattgt ttgattcctt gtaatccaaa agaaactcct tactgtattt ctcaagctct
     4021 aatcaatgca gttaaaggag atgttgaaaa ttcactttta ttttgtggaa atgatgccta
     4081 taaaattgat aaattaagca ctgttgaaga tgttatcaat gaattgattt ctgaattata
     4141 attttaaaaa ttttatcaag ctaaggagtt taaatacgtt attcattaat ctttataaac
     4201 atatttacgt ccttagcttt tttgtgtcaa tttcctttta tggagatttt aatttaatta
     4261 tagatagtat taacctttat agctaatctt cttttataag tacattggtt atttcagcta
     4321 tatataaagt atgataatct ttttgtggat accatcttcc atctatacct tcagcaataa
     4381 aacattgagg ttcaagtttc tgactataca tttttttgca tataatagcc attttagctt
     4441 ctttaaaata aggtgtatca tttaagtgtt ctatattaaa tccaatctgt gatatcttat
     4501 cttcttctct accagatact tttccacaat atgaaagctc tttacgaaaa tcttcatcaa
     4561 aaaatgtaag agaaaattta tctgtattat ctataaattc cttagtataa cgctgtggtc
     4621 ttattactac aaaagcaaca tcttttcccc acataactcc aagccctccc cagctagcag
     4681 tcattgtatt cactttacct tcattttctg ctgttataag tgtccagtct ttacctatca
     4741 atccaaatgg attattttta atctcttctg ccaaaatctc tttaaaataa ctcatatctc
     4801 taataacctc t
//
LOCUS       DAEMAE010000248         4808 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEMAE010000248 DAEMAE010000000
VERSION     DAEMAE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.251"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..755)
                     /locus_tag="KPH93_003299"
     CDS             complement(<1..755)
                     /locus_tag="KPH93_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG1690071.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNV"
     gene            1029..1703
                     /gene="fsa"
                     /locus_tag="KPH93_003300"
     CDS             1029..1703
                     /gene="fsa"
                     /locus_tag="KPH93_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG1690072.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1825..3219)
                     /locus_tag="KPH93_003301"
     CDS             complement(1825..3219)
                     /locus_tag="KPH93_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG1690073.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTLVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYVLGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEVNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLVKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3297..4643)
                     /locus_tag="KPH93_003302"
     CDS             complement(3297..4643)
                     /locus_tag="KPH93_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1690074.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         1864 a          678 c          627 g         1639 t
ORIGIN      
        1 tttacattaa attctgataa aatatctact atatcaggag taggcgcttt ttctgtaatt
       61 atagaactaa tatcttctaa atgagcaaac ttgaaatttc catcaaattt aaacttttta
      121 tcttccataa ctataaatac ttttttactt gaatttatta tagccttctt tgtgagacca
      181 tcttcaattt caaaagttgt aagtgaagag ttgaatacat ctactccaca acttcccata
      241 aaagtcttat caaacttata tttcgatata aactctatag ttggagtacc aacaaaaccg
      301 tctaaactga gatttaataa acctccagtg gaaattatat ttaaattatt tggttttgaa
      361 gtaactacat ttagtatatc caacatattt gtaacaagtg ttactttttt attactttca
      421 gctaacaact ttgcaagcaa gatgttaatt gtagagatat ctaaaaatat agtttctcta
      481 tcttcaatta aattaaatgc tttttctgct attattttct ttgttggaat attaatctct
      541 tttctgtctt ttgtatcctg attctctagt gtttctctag ctagtacagc tcctccataa
      601 attcttctta ttgaagaaat cttttctata ttttttaaat cttttcttat acaatcttct
      661 gtaacattaa atttagaact gagctctttg acagtaactc taccttcttt ttttaggata
      721 tttaatatag cttggattct ttcttctgca aacattttct acctcttttc gcaatatagt
      781 attatagtaa atattataag ataaaataca atatatcaac tattttaaca tgaaatacaa
      841 aattaattta atataaaaag tatgtttttt ctaactatat atttagaatt tagtaaataa
      901 aaaaattata aaactaagaa ggaaaagtaa aacaataata aaaaataaaa aacaataagc
      961 aaaatgttga aaataatacc aaatcaatat ataataagaa taaacaaaga ttaaaggggg
     1021 atattttaat gcaactaatc ttagatacag gtaatgtgga ggagattaag gagttatgta
     1081 cttgcttacc aatagatggt gtaactacaa atccaagtat tgtttcaaaa gaaaaaaaga
     1141 attttaagca acttataaat gaaattggtg agattatagg tgaggatatg cctatacatg
     1201 ctcaagtgtt aagtacaaag tatgaagaaa tattagaaga agcattatat ataagtagtt
     1261 tgagaaaaaa tatatatgta aaaatacctg taacacaaga tggtttaaga gctataaaag
     1321 accttcacag aaaaggtgta aaaataactg ctactgcaat attcactgct catcaaggtt
     1381 ttttagctgc aaaggctgga gcaaattata ttgcacctta tgtaaatagg cttgataata
     1441 tatctgggga tggagtagct atggtttcag aacttataaa aataatagat acttataaaa
     1501 tggatacaaa agtacttgca gccagtttta aaaatgcaca acaagttata gaattaatgc
     1561 aacatggggt gcatagtgtt acagtaccat atgatatttg taaatctatg atgaatcatc
     1621 cattaactga ttggagtgta gataagttta tagaggattg ggagaatact tttggaaaag
     1681 gaagtaaaac aaataatatt tagtattatg gtaaaaaagt agttttgtcc aatttcacaa
     1741 gaacttatat aaaaagatga aaatatgaat tttcttttta ataatttcgt tattatctta
     1801 agtcaattca tattttcatc ttttttagat ttctagtcct ataaatttcc accaagggaa
     1861 tgttatacat attattataa agtaggttaa aggaatcaaa gttaaaccgt atttgaaaat
     1921 aaccttgcta ttatacagat tttcaccatg accaaccatt attgtagcat gatgaaatgg
     1981 caatatgtac tgtatactta ctataatgta gacaaataga cataaagtaa cactattttc
     2041 ctttagtata tgtaaacttc caagtaacgg tattactaca gatgatgtag tgacagcact
     2101 tccaagacat atattcaaga ttattgtgag agttatcaaa aacaatatcg aaataatttc
     2161 tgaagagttt ggtataaact ttaccaaata atttcccatt atatctacaa ctccagagta
     2221 ctttaacact cctccaattg aaaatgctgc tataaaaaaa ataaggacct tccaatttat
     2281 ttcttttaaa gtactaaatc caactatttt tcttagtaat aaaagaagta cacaaattga
     2341 cattataatt aatgtgttta tgttatgtat gaactgagtt aaaaacataa aaaacattat
     2401 tcctaggcat aaaaatacgt gtttttgctc tttgtttagc aaattaaatt ctttttttaa
     2461 attagaatat gaaaaatgaa gtttattaac ctcaagattt tcattagaaa taaattttat
     2521 tgaatgcaat tcttttttaa agacaaagat aaataaaaaa taagttataa aacaagtaat
     2581 tatagttgga acagacatat aaaaagccca ttgaccccaa tttatattac aatttccaag
     2641 ttctagtaca acatagttta gaagggtgtc tccatttaca aaaaacattg atgtaaaggt
     2701 agatgctgta aatatactaa ataaaagtat agattttgaa tcttcagtta tctgttgttc
     2761 ttttaaaaac tccttgtaaa aagcagatac aagaataacc cttggaaatg gttgtgggat
     2821 aaaaaatatt aaaagtatac ctaaaacata cgataaaaga atcatttttt taggggtatt
     2881 tacagtattt aaaattaact tctcagaaat aatttttgcg acacctgttt ttgtaactgc
     2941 attagtaatt atgtatgcaa gaataattat gtaaaaattt tctgtaaata aaaactgtaa
     3001 aataaccttt attggtgcaa tgttaaagat aaacgctaga attacaaaaa acaatgcaac
     3061 aagagttttt tcaactgagt ctacagccca tatcaagata gttaaaaaag ataagcatat
     3121 agaaaatttt atgttgaaac tgtaattaag agagaagtag agaattatgg tagctataac
     3181 agtcatataa aattgttttg gcttaagaat aatacccatt tttatattca cctccataat
     3241 ttattatatc ataatggagg tgaatacaat gaatgtgaat acattttata aaaagattat
     3301 tcattgattc tgtgatttac aatagcttta gttttccaat accctttctt atagtagata
     3361 actgtaacaa taagacctaa agtccaacca aaaggaatac ccatccaaat tccgtctgtt
     3421 ccaaaaaatt tggaaagcat ataagaagct ggaagtcttc caagccataa agatactatt
     3481 gagcttataa gtgcaaacat ggaatcacca gcacctttca ttgcacttga aagtacaaaa
     3541 gaagttccaa taaataaaaa gaatggtcct attataaata agtaagaaga gccaatttta
     3601 atgacatctt tatcagtatt gaatagagca attagatttt ccctaaatag caaaattaaa
     3661 gcaataactg taactgctaa aactaaggtc atttttatag attctcgaat gcctttttta
     3721 actctttcat tttctccagc acctatgttt tgagcaacaa aagtagaaac agcagatcca
     3781 agattcataa taggtaagct tataaaattt tctattctaa gaccagcacc aaaagctgcc
     3841 atagcagatg taccataact attaactaaa aattgaagag ccatatttcc aatagaaaac
     3901 aacatttgtt gaactccact tggaagtcct aatctaagcc catctttaaa tattttgtta
     3961 tcatatacaa ttcctttgat tttaaattta agcacttcat gttttttatt taaataaatt
     4021 atacttatta taaatgatac tgcctgagcc attatagtgg caagtgcaac tccctcaaca
     4081 cccattctta gcactacaat aaaagttaag tctaaaacta tatttagaat tgtggctatt
     4141 atcagaaaat aaagtggagt tactgaatct ccaagtcctc taagtattgc acttactgag
     4201 ttatatccag cagaaaataa tattccacta aatattatag taagatacaa ttttgattga
     4261 gcaaaaacgg attctggtgt attcataagc ttaagcatgg ggccaccaaa tactattcca
     4321 agaatactta ttaatataga accaataaat aaaagtatat atgtagtatc aatagtagct
     4381 tttacctttt ttaaatctcc tgagccataa aattgtgaca ctaaaataga aaatccaagg
     4441 ctaacaccca taagtgctgc caccaataaa aacattatag ggttagctcc agagactgcc
     4501 gccattgctt cttttccgat aaatctacca actattatac tgtcagctgt attgtatagt
     4561 tgttgaaata aactaccaat aagcataggc atggcaaaaa agaatatgga ttttccctca
     4621 tgacctgtag ttaaatcttt caccttattc ctccaaataa aaaattatta atttattata
     4681 gtataatctt aacataaact ttatatccaa actataaata aattgtatca aaatatcata
     4741 tattttttta aaatgtaaag aatataatta taatttgaat ttaataaatc aatatttaat
     4801 ataatcga
//
LOCUS       DAEMAE010000249         4793 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEMAE010000249 DAEMAE010000000
VERSION     DAEMAE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.252"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..842
                     /locus_tag="KPH93_003303"
     CDS             39..842
                     /locus_tag="KPH93_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690075.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYIDN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEIAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFINDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            854..1984
                     /locus_tag="KPH93_003304"
     CDS             854..1984
                     /locus_tag="KPH93_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1690076.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESEHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNNYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1988..3397
                     /locus_tag="KPH93_003305"
     CDS             1988..3397
                     /locus_tag="KPH93_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690077.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMLREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKDLKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEVTIIECDNEIRRVYKVKTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGESKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3608..4117
                     /locus_tag="KPH93_003306"
     CDS             3608..4117
                     /locus_tag="KPH93_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG1690078.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         1998 a          395 c          840 g         1560 t
ORIGIN      
        1 tatagatata aaaataatta tagaaagaaa ggcacattat ggaaaaattg agagttattg
       61 aaatgataaa aaataatccc aatattatag ctacaataga taatccaact gatgaaatga
      121 aattgttagc tattaaagaa aatgggcttg tactagaata tatagataat ccaactagag
      181 agatgcaaga actagcaata gataataata ttagagcaat taaatttata gataatccaa
      241 cggaagatat gatgataaaa gcagtaaatg agggatggag tatactggat tatatcaaaa
      301 acccaactga taaagttatt gaaattgcta taaatcaagc aggatgggca attaaatatg
      361 ctaaaaatcc aagtgaagaa ttacaactat tagcagtaag aaaaaattat gattcgataa
      421 agtttataaa acaaccttat gaaagtgtac aagaggaggc agtgagaata agttatgatg
      481 ccttgagata tatagattca cctagttata atgcagaact tatagctata aaaaataatg
      541 aagcagctat tagctttata aatgatttag ataaaaataa aatgttggaa tttctaaaag
      601 tgaatatttt agtgattaag tacattctga aaaaagtatc taaagatgaa ttagagagtg
      661 tattaaaaga agtattatcc aaagaagatg ttgaggaaaa gtatattaga gattttttga
      721 attgtagtgt tatagataga aatagtgaaa gtataccaat agataaaatt atgtttattt
      781 ataaatatgg aagtaagaca gcaaaaaaaa tagctgtaga tgagaaatta aagattatgt
      841 aggagagaga aaaatgaatt ttatagacac attaaaaagc gttgacttaa tattatccac
      901 tggagaagta cctttaatag taggtgaaag tggaatagga aaaacagctt tagcaaataa
      961 gcttgccaaa gaaaatgatt ggagtttaat tgttattgat ggaaacttac ttaaagaggg
     1021 tgaaatagga ggtcttccaa ctatagaatc ttatgtagga gttaattcta atggatataa
     1081 gactgaaaag aaaacaacag tgtatgctgt tcataacaaa ttaagagaaa ttgatgaaga
     1141 gatatctaaa agtaagacag ttcttttatt tatagatgag ataaaccgtt gtgagcatac
     1201 agtacagcaa gaacttatga atttaatttt aaatagagaa attaatggat ataaactgca
     1261 tgatgatgta aagattttag cagcaatgaa cccatcaagt aaatatggtt cagattttga
     1321 ttatcaagtg gtagatatgg atgcagccca agaaaatagg tttgtttggt taaatatgga
     1381 gagtgagcat acacaatgga taaaatgggc aatagatgag ggtattgaaa gaaaagttat
     1441 cgagtttatt tccacttttc cagaatatct gcataaaata aatgaagatg atgtaagagc
     1501 tactcctaga agttatgaga gagtatctaa aatttataaa gtttataaag aaaaaaataa
     1561 ttctattcct agagcagtat ttttaaatgt tgtaaagggt aatgtaggta aagttattgc
     1621 agaagaattt ataagtttta ttgaatcaaa ctctgaacca ttaatatcat atgaggatgt
     1681 ttttttagga gaatctattg atgagtccat tgtagaaaga gtaaaaaatg agagtcatac
     1741 aagactttat ctatcagcta tgaacattct aaaagactta gaattaaata ttaagaatga
     1801 taaatatgaa tcaaacaact atattaatag atttattgaa tttttaaaaa tgtatcctgt
     1861 agatttaatg ataggtatta tgaaggatat tagaaacagt tatattgaag tatataaaaa
     1921 agctatagaa aatgaagagt ttgtaaaatc atattttgaa tcttatagtt taataagggg
     1981 ataatgtatg gaaaattatt ttgataaaca agcaaaagag ctttataata aagcaagtga
     2041 aattataaat actcattcta tgctaaaggc aaatcgtgct aatgagaaat ttgatataga
     2101 tataccacaa gattttaagt atgagttttt tagtcttgta gataaagtga atttaagtct
     2161 tatggaagaa gaagataatt tttatggata ttttttgttt cagatgttaa gagaaataag
     2221 atttgatata agtagtccta caggtgtgaa ttttaaggga gctaaatatg ttatatattt
     2281 taatccaata atctttttaa cgcttgatat aaagcaaatg gagactacaa ttaaacatga
     2341 aattcatcat atattatcta tgcatctgat gagagcaaag gatttaaaag gaaagtatag
     2401 taccttggca attaatatgg caatggatat agtagtaaat aaatttctaa acaatttacc
     2461 accatatgca actaccttag agtgggtaaa tttaaaatat tccttaaaac ttgaaccata
     2521 tgaacctttt gaatattatg tagaaaagat tcagactgaa ttagatttga tggaagtgga
     2581 tgaagatggt gaggaagatg atagtgataa atatggagat atagaaattg attatagccc
     2641 cgaaagaact catgatatat gggatgaatc caatgagata gatgaaaata cccttagaga
     2701 atttactgaa aaatttatta acagttctca aaaaggtaaa gttcctgctt atttagaaag
     2761 tatgatatca tcacttaaaa atagtaaggg tgaattgcca tggaatttat atcttaatag
     2821 aataatggga acagttgaaa ataataaaaa gaagactata acaagaagaa atagaagaca
     2881 accaaatcgt ttagatttga gaggagaact tagaagtcat aaggcagaaa tagcagttgc
     2941 acttgatgta agtggaagta ttagtgatga agagtttaaa caagcgatta aagaagttct
     3001 tagtatagtg aaaaactata atcatgaagt tacaattata gaatgtgata atgaaattag
     3061 acgtgtatat aaagtgaaaa ctgaaaaaga tataaaagat agaattagga taggtggagg
     3121 tacaagattt tctccagtat ttgagtatgc taataataaa aagattaatt tgttagtcta
     3181 ctttactgat ggtaaaggtg aaagtaagtt acaagtaata cctagagggt ataaaatttt
     3241 atgggttatt tctggaagag gagataaaat ttcattaaaa gaaccttatg gagcagtaaa
     3301 aaaacttact aaagttaaaa caaatgatga aatattagaa ataaatgatg taagaaatga
     3361 tgggtactca atgaatagcc aagcaccaat tttataggta ttcacaggag ataagctgtc
     3421 ttaatttatt ttgatatgct tgttataaaa atctaaaaaa tgcattaatc aatggttaat
     3481 gtatttttta attttaataa tataagtttt acttacatta aagtctcaaa atgtgttaaa
     3541 atataatata tagcagatag ttttggcaag gtggatttag ttgtcaaaac tatttttttt
     3601 attaaggatg gtgatgtttt tgtttaagat tgatgattat ataatgtatg gtatgacagg
     3661 agtctgcaaa gtcatggata taacaaatga acgatttaca aatggaataa aaaaagagta
     3721 ttatgtattg agtcctattt attctaataa tacagtaata aaaattcctg tagataatga
     3781 aaaagtacca atgagaaagt tgctttctaa aattaatgtg ctttcattaa taaatgatat
     3841 tcctaatatg gatacatcat ggatagataa tgaaaaattg agaagtgagc aatttaaaaa
     3901 aatactcaga ggtggtaaat gtgaagagtt aataaaatta gtaagaagta tagataataa
     3961 cagagagtat gtaaaatcta ttggtaaaaa gacacatcaa gcagatgata atataatgaa
     4021 agaagctgaa aggttattaa atgaagaatt tgcaactatt ttagatattt ctcctaatga
     4081 agttagttca tatatttcaa gtcatatacc tcaataaagt gttttagtat cctttattta
     4141 atctatttta agtattattt atgtatgctg gttttgataa aataaaatat ataattaaat
     4201 tatataaaaa attaagagta attgtattga agaattacta taagatataa tttcttaaag
     4261 atttaaattg ttattgtaga tgtttttata ctttttaata taaagtaaga ttaataagtg
     4321 tattgtaagc tttaagtgaa attgatactg taatgagtta tttagatttt aaaatgtata
     4381 atattataaa aaatcctttt aggttttgaa tgaataagat ggatactatg ttgaatagaa
     4441 tccatcttat ttaaatttta tttatgttgt ttgtaagtat tatttttgta catgaaaaca
     4501 cagtgtaaaa aatatgctta gaattatgaa gttttattaa aaatattcga caaaataaga
     4561 ctaaaaatat tcttagactt ataactaaga taaatattgt atatttatag tgtagcctga
     4621 aaaattgtga atgtacgaaa tttatttagt attttattga aacaaagtag aagaagcact
     4681 atagtttatt tttgaataga ttgttatttt ttagtgaaaa agttattagg ttaacacata
     4741 aatataattg cttataaata gtttaagcat attaaaaaag tagttattag ata
//
LOCUS       DAEMAE010000250         4732 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEMAE010000250 DAEMAE010000000
VERSION     DAEMAE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.253"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..629
                     /locus_tag="KPH93_003307"
     CDS             <1..629
                     /locus_tag="KPH93_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG1690079.1"
                     /translation="KIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQF
                     LALKAVLNMDGVFATNVKKVNMIYGICTFTTFKMLNSNHFFLTSKKEQRGALCITIDA
                     YGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMAI
                     SLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            869..2128
                     /locus_tag="KPH93_003308"
     CDS             869..2128
                     /locus_tag="KPH93_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG1690080.1"
                     /translation="MGRRATRVSRLGKEKKRSVLIVLAALVIIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSIVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTIYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVNQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2387..3382
                     /locus_tag="KPH93_003309"
     CDS             2387..3382
                     /locus_tag="KPH93_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG1690081.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKVKKKLHKKLVVAGLAIVMISMIIISSII"
     gene            3628..4695
                     /locus_tag="KPH93_003310"
     CDS             3628..4695
                     /locus_tag="KPH93_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG1690082.1"
                     /translation="MSNIKIIASNKCWIENIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGILKKKFKKDKWIKLLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKNHDNALIWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPQAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
BASE COUNT         1963 a          468 c          799 g         1502 t
ORIGIN      
        1 tgaaaatagt aacctacaat attcataaag gtatggattc aaataataga ttaactctaa
       61 ctaaaatggg gttatattta aaacaattgg attgtgatgt aatttgtttg caagaagtat
      121 tatatcctca atttttagct ttaaaagctg ttttaaatat ggatggtgtc tttgctacaa
      181 atgtaaaaaa agtaaatatg atatatggta tttgtacatt tacaacattt aaaatgttaa
      241 acagtaatca ttttttctta actagcaaaa aagaacaacg gggagctctt tgtataacta
      301 tagatgcata tggaagaatt ataaatgtaa taaatactca cttgggactt gatagacagg
      361 agagagcaaa acaattagac gaaataatag actataggaa cagattagtg gggatagtta
      421 ttttatgtgg tgattttaat gagaaaaatg tctttttaag tatgtttaat gatatggcta
      481 tttcactaaa caagtcttac ttaccaacat ttgaaaaatc aaactctaga atagattaca
      541 tttttgttaa taaaaatact gaactaaaag gatacacagt agaaaaaatt tacctgtcag
      601 accattatcc tgtaataggt tacatttaaa tttcaatata aatgaaatta gttgttataa
      661 aataaacaat ttttttgtta agaaatagta aaagaaatat aataaaaaat atcaaaaata
      721 aaactaatta taagacataa atttacaata aagttacatt tgattaaatt ccttgtttaa
      781 caaaagctat tcaaatataa tctaaggtag ttttgaaatg ggaactctat tatgcaaaat
      841 taagctagtg ttaaaatgag gtgaagttat gggcagaaga gcaacaagag tttcaagatt
      901 aggtaaagaa aaaaaacgaa gtgttttaat tgtattggca gctttagtta taataatggg
      961 tggatttact tattttttta acagtaaatt tctgtacaat gggaaaatcg ctaaaaatgt
     1021 atatatagaa ggagtaaatg tatctgatat gactaaagca gaggcactaa aagcgataac
     1081 agataagtat actccagaag acttaaatct tacttatgat gggaaaaaat atacaatatc
     1141 accaaaagat attgatttaa aatatgatac agaagatgtt gtaaaagatg cttatgaaag
     1201 tacaaaaaaa gggtcctatt ttcaaaactt aaaaaaatat attgatataa gagtgaataa
     1261 ggcaaatatg aagataaaag ctgaatatga tgaggcaaaa cttagttcca aggtgtcttc
     1321 tatagcagac agtataaatg ttaagatgaa aaatgcttct attagtgttg gaagtggtgg
     1381 actaagttat acagactcta ttgtaggtag agaatttgat ttggcagcaa ataaagagtc
     1441 tatatataat atgataaaaa ataaagagca taaaacttta gaacttaaag taaatcttca
     1501 aaaaccagat ataacaactg aacaagtaaa aactgtaaat tctgttatag gtcaatattc
     1561 aactacttat tcacaggctg tagagggtag gtcatataat gtaggattat cagcaagaaa
     1621 aactagtgat gtattattaa tgccaggtga agaattttca tacaataaac ttacaggacc
     1681 tagtaataaa gcaaatggtt acaaagatgc acctgttata gtttatggta agttagaaca
     1741 atcagcagga ggaggtgttt gtcaaacatc ttcaacaatt tataatgctg ctcttctctc
     1801 aggtatggag ataactcaag ttacaaatca ttcatcagca tctacttatg tacctaaagg
     1861 gagagatgct actgtgagcg atgggggatt aaacttaaag tttaaaaatc catataagca
     1921 tcctgtatat attaaaaatt atgcaggtgg aggaagtgta tcatcagtaa tttatggtaa
     1981 ttctggtgat aaaccaaata tatctataga ggtaaaccaa actggtcaga ataaatattc
     2041 tacatatcga atatttaaag attcaagtgg aaaggttata aaaaaagaac atatatcaaa
     2101 tagttcttat aaagaattaa aaaaatagat aattttttag agaaatattt gtagtataaa
     2161 agggagtgtc tcaaaatgaa ctttttagtt tatgagacac tctttttatg tgaaatcaaa
     2221 tatattttca tcatttcaac aatacaaaag aagtgtatct ctattttgag acagtctttt
     2281 ttgtaaaaat taacactagt agttcttgtt ttaatattac aatcattaat atatagaaaa
     2341 tattcctata tattggtata attgtaaata gatttagtta tataaaatgt atgaggaggt
     2401 gacatggttg aattttatag gcaatagata tgaggtagta aattctgatg acgttttaga
     2461 aattaacaaa atctataaag ctagagatgt tttttacagg aaaaatgtgt taataaaagt
     2521 tataaagcac aataattata tctgtgaaga ttttgtttca aatttaattg atgaaagtac
     2581 tgctttagat gagataaact ctccttatat actaaaaata atagatgttg ggattcactg
     2641 tacagaagaa acaacattat attatgttgt aagtgaagac tttaatggta tcggactaga
     2701 tgaattaatt ctaggtaatt atcttcattt agaagcaatt gtaaacataa tgatacaagt
     2761 tttaaaagct ttagaaatga tacataggaa tcattcatat catggtagtt taaaatcaag
     2821 tagtataata gtagatacag aatacaatat aaaaatttgt gattttggaa ttacaaaagc
     2881 aaataatggg gttaacacaa gaagctatgg aaacaggaga tatttatgcc cacatcaact
     2941 atgtataaat tatactgata aagaaagtga tttttttgca acaggtctta ttctatttga
     3001 atctatattt aaaaaattgc cttttggaga atcaaattca gaagaaaaaa tgctacaatc
     3061 tattgataag ggattagatt ggaatagtat aaaagcaatt aatggaaata cagaacttat
     3121 aaatgttata aaaaggcttc ttggaagaaa taataagtat actagagcca atgatattat
     3181 tattgattta agtaaagtaa tgtatgaaaa agcatatata gaagttgaag atgagaaaga
     3241 agaagaacag aaacaagaca aagttataca ggttaaaaaa gaatataaaa gtaaagtgaa
     3301 aaaaaaatta cacaaaaaac ttgttgttgc tggtctagct atagtcatga tttcaatgat
     3361 tattattagt tccataatat aaaaattgta tattgagtat tttaaaatca atgtctataa
     3421 atgatataca tagaaatttt agtgtagtct taaattatga aaggaagtgg ttattatgtt
     3481 aataaaaaat aatgaggtat taaacgtcta ggagtatttt aacaaatagc cacctgcttt
     3541 tacagtttag aataaaaaag ctgtggctaa aggtcacagc tttttgctac tttttaaagg
     3601 caggagcata ttaaaaagga gcaaattatg agtaatataa aaataatagc aagtaataaa
     3661 tgttggattg aaaatatagc aaggaaacaa cttgaagata tttcaaagtt aaatggaatt
     3721 ttaagagtag taggattacc tgacctacat gcaggtaaaa tacctgtagg tttagcagtt
     3781 gagactaaaa atattattta tccacatata attggaaatg atataggatg cggaatgact
     3841 ttatttaaaa ctgggattct aaaaaagaaa tttaaaaaag acaaatggat taagttactt
     3901 agtaagatta aagatttgtc tgatatagaa ataaaaaaca catataaaga agaatgccca
     3961 attttaaatt taggaactat agggagtgga aatcattttg tagaaattca atgtataagt
     4021 gaaatttata ataaagaaca atttgaacaa ttaaaatttt catctgatga tattatgatg
     4081 ttagttcact gtgggtcaag aaattatgga gaagatattt taaaaaaatt ttatgataaa
     4141 gacggacttg aagttgagag tgagaaagcg atagattata ttaaaaacca tgataatgct
     4201 ttaatatggg cagagcgtaa tcgtgaagct atcagtaaaa aaattatgca gtcaataggt
     4261 acatctggag atgttgaaac aatattctct ataaatcata actttataga gaaaagggaa
     4321 gataaattta tacatagaaa aggtgctgtt tcaagtgaaa gaggagctgt gattatacca
     4381 ggttcaagag gcagtctatc atatatagtt atgcctactg aaaatacaga gatatcttta
     4441 tattctctat cacatggagc aggtagaaaa tggtctcgtt cagtttgtaa atctcgactt
     4501 aaaagtaagt atagtaaaga tactataaaa caaactaaat ttaaaagtca gattatatgt
     4561 aatgatgtaa atttattatt tcaagaagct ccacaggcat ataagaatat agagcaaatt
     4621 attgaaagtt tgatagaata tgaacttatt caagtagtgg caactatgaa gccattgatt
     4681 acttataaag gataattttt aaagttaaaa aagattaaaa ttatattata aa
//
LOCUS       DAEMAE010000251         4726 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEMAE010000251 DAEMAE010000000
VERSION     DAEMAE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.254"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..734
                     /locus_tag="KPH93_003311"
     CDS             162..734
                     /locus_tag="KPH93_003311"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG1690083.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            798..1448
                     /gene="fsa"
                     /locus_tag="KPH93_003312"
     CDS             798..1448
                     /gene="fsa"
                     /locus_tag="KPH93_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG1690084.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            1600..4524
                     /locus_tag="KPH93_003313"
     CDS             1600..4524
                     /locus_tag="KPH93_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1690085.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         1913 a          434 c          924 g         1455 t
ORIGIN      
        1 atattaagtt aattactttg aattttaagc tcctgtctat gatatagaca ggagcttttt
       61 tagtatttta atataaaata cttacaagaa tagatagcaa aattagaaca aatataattt
      121 ttttattaat ataactttag aaagggctag gtggaagttc aatgaaggct ttacaagaaa
      181 agatattgag agaaggcagt gtttcaggaa atgatatact aaaagttgat agttttttaa
      241 accatcaaat tgatgtagct tttttaaatg aaataggaag agaatttaaa gagagattta
      301 aagaagaaaa ggtggataag atatttacaa tagaggcttc aggaatagct atagcatcaa
      361 ttgtttcaca gtattttgat aatgcacctg tagtatttgc aaaaaaatca gaatctaaaa
      421 atttggataa agatgtatat gagactaatg tatattcatt tactaaggca agagaatatt
      481 cagttaaagt ttctaaaaag tacataaaca aaggtgaaaa tatattgata gtggatgatt
      541 ttttggcaaa tggaagagct gctcttggac ttaaagattt aattgagcaa gcagaagcta
      601 acttagttgg agtaggaatt gtcattgaaa aaggatttca agctggagga gcacttttaa
      661 aggctaatga tgtaagatta gagtctttgg cggtagttga atctatagat aatggaactg
      721 taaaatttag gtaatttatg atattatata cttgtgttta gcacatattt taactatgta
      781 attatggagg attttttatg agatttttta tagacacagc caatattgaa gaaataaaag
      841 aagcaaatga tttaggtgta atatgtggtg taactactaa tccatctcta atagctaaag
      901 aaggtagaga ttttatagag gtagttaaag aaataagtga aatagtagat ggaccaataa
      961 gtgcagaagt aataagttta gagcataaag gtatgataga ggaagcagaa aaattatcta
     1021 agatacataa aaatatagtt ataaagatac ctatgacagc agaaggtcta aaagcggtga
     1081 aagtactatc aagtaaaggt ataaagacta atgtaacact tatattctct gctggacaag
     1141 ctcttttagc tgctagagct ggagctacat atgtaagtcc ttttgttggt aggttggatg
     1201 atatatcaca aaatggtctt gatttaattg aagaaatcgt tgatatattt agtgtaaata
     1261 gtattgaggc tcaaataata gttgctagtg ttagaaatcc aatacatgtt ttacaagctg
     1321 ctagaatggg agcagatata gcaacagttc ctcttaaagt tataaaccaa atgattaagc
     1381 atcctttgac agacaaagga atagatagct ttatgaagga ttgggaaggt gcttctttaa
     1441 aattataatt ataaactacg ctttataagt atattaaaga ggttattatg gaaattctaa
     1501 gtaataaggc taggctattt tgcctagtct tattttggtg tataatatat tgaaacatat
     1561 tattgaaata gattaatttt ttaagtgagg aggcgaaaaa tggataaaat attaagagag
     1621 atacaaagag aagataaaaa aaatccatat actgaccaag aacttgctga aatgttaaat
     1681 gtagctaggt ctgaagtgat atcagtgagg aaaaaaaata atattttaga ttcgagagaa
     1741 agaagaaaaa aaatattaat aaaagatata tcaaaaattg taagtgaaaa tcctcaaatg
     1801 tctgaaagaa aaataactga gatattaata gagaatggat ataatatatc gagaagtgca
     1861 gtttcaaaat tattaaaaga ggaaaattta aatcagtcaa agattgggat taaagataaa
     1921 gagccagcat catcaggaaa gtatgtgaag gtgagtaaag ttgtagaaga agatggattt
     1981 gaagaattga taggtataaa gggaagtttg aaagaaaagg taaatctagc taagtcagca
     2041 ataatgtatc ctcctaatgg ccttcatact ataatatatg gagaaacagg ggtaggtaaa
     2101 agtgaattag ctacatgtat gtataaatat gctataaaaa acaacatcaa agaagagaac
     2161 agcccattta tagtgtttaa ctgtgcagac tatgcagaaa atcctaatct attgatagct
     2221 caactctttg gggtagtaaa aggtgcatat acaggagctg atgcaaatag agaaggtctt
     2281 gtagaacaag ctaataatgg aatattgttt ttagatgaaa ttcatagatt gcctgctgct
     2341 gggcaagaaa tcttattttc attaatagat agaggagaat ttagaaggtt aggagaaagt
     2401 agttctaata gaaaggcaaa tgttctcata ataagtgcta caacagaaaa cccagattct
     2461 aatttacttc aaacatttag aagacgtata ccaatggtaa taaatttacc taatatgagt
     2521 gaaagaccaa aatctgaaag atatgaaata ataataaagt tttttgaaag agaagcaaag
     2581 cgagtaaaca agaattttat aataactaaa gaagttatgt gtgcacttat gaattataag
     2641 tgtactggaa atataggtca gttaaagagt gatatacaag taacttgtgc tagagctttt
     2701 agcaaatcaa tattttctag cgataaggta attatagatt tagatagttt aagggattat
     2761 ataaaaagtg gatattatga ttgtaattgt aaaaatgaaa agtattctga gttttcagtt
     2821 gaggatatat atatagatat cagtgaattg gtgagtggaa aaaatagaat tttatctgat
     2881 aatgagataa gcgagatata taattatgca gaaaaagagc ttaaggtttt ggaaagtaag
     2941 tgcttttcag aagaagagtt aaaaaatgca tttatagaaa agttagataa gaagtttaat
     3001 gctattaaaa acaataaaaa tttatctgat agaagaagtc gaatagactg gggaggacaa
     3061 ttaaaagagt ctacattgga gataatggat aaggtaattg tatttattaa ggagcagttt
     3121 aagcatgtaa atcaaggatt gtacttggct ctagcaattc acattgagca tgctataggt
     3181 aggataaaag atggaaaaac tataataaat ccgagtttag ataaaataaa agcatcaatg
     3241 ccaatggaat atgaattgtc aagatatata atgggtatag tggaagattt gacacaagta
     3301 acatttccag aagatgaact tggatatttg gcttattata taaataaatt ttgttataat
     3361 gaggaaagta taaaagataa agttaaagta gtaatagtta cgcatggtaa agttggaatt
     3421 gaaatgtcta aagttgtaaa tcatatttta ggtatagaat gtacactagg tatagaaata
     3481 gcacttacag attcaccatc agaggggata gagcatgtat tagaagagct tcaaaagata
     3541 gaagctagaa aaggtattct agttctaatt gatatgggtt ctttggtaat tcttggtgat
     3601 gaggttgaaa aaaggcttgg aattagatgt aaaactgtaa atagagtaga tacgttatta
     3661 gcaatggaag caggaaaact tgcaaccatt gaaggaaaaa gtttagatgg tataatagca
     3721 gatttaaaga aaaataagaa ttatgccatg ataaatacaa ataaatttag ttacagaaaa
     3781 aacgagtatg gaaagaaaaa tgtaatcatt actttatgtt tgagtggtgt aggtacagct
     3841 ttaaatttaa aagagcatat agaaaaacaa attaaggaat atgatacttt aattgaggta
     3901 aagccagttg cttttttaaa taataattta gaagaagagt taaatgatat tgaagctgaa
     3961 tataatatta ttgctatctg tggaactatc gatatagatt ataaaaacgt accttttatt
     4021 tcttataatg aggtacttgg taaaaatggt attaataaag tattagagca tttaaacagt
     4081 aaacaaccag ctttaaatat tgaaaaccag ttgaataatt taatacatga agatttgatt
     4141 ttatacaatt ttgaaggaat ttcaaaggag tatataattg atactcttgt ttcaaagctt
     4201 gaagaaggtg gatatgttga ctcaaagtat atattaagtg tatataaaag agaagcaatg
     4261 ggaagtgttg taatggcctc taaggtagcc gtgccacatg gacttccaga aaacgttata
     4321 aagcctgcaa tagcaatcgc aaggttgaat aagcctatag tgtgggacaa taaatttatg
     4381 gtggatttgg tagtactact ggcacttaaa gaaaataata agaaagaaat acgaagctta
     4441 ttttcaaaga ttaatgacca gcatacttta gaagttttat taaatactga agataaaaat
     4501 gaaataaaag aacttttaag ctaattatta gtacagagaa gaaagacaaa aaatttgctt
     4561 tagtataaaa tttaaaacta cttaattgct aagtagttaa ttactcgata taaggctagt
     4621 tctatgaaat ttatttatat gattttatag aattagcttt tttcttggta aataaaatat
     4681 atgaatttat ttatgcaaaa agtaaatttt attggacaat tctatg
//
LOCUS       DAEMAE010000252         4725 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEMAE010000252 DAEMAE010000000
VERSION     DAEMAE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.255"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1202
                     /locus_tag="KPH93_003314"
     CDS             <1..1202
                     /locus_tag="KPH93_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1690086.1"
                     /translation="LNENRLVLTDIEFKSVLNNILVMLSRHKYNEKEFIENYIENYRS
                     KRSSILENEQNKEVIINSIKTFCKNLKLATSIDISNDKVFEEFLYKHISSLCKKIKLG
                     INQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINEDEVANIALHIGGAVERASY
                     NNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILKIIPSYLVEYIKVIDVDFVI
                     STVPIEVGDVPVINISPILSEKEIKLIEKYIETGNIYLDLDIKSLFDSELFFTDLVFK
                     RKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIGNMVTIPHGAIGKIFDNKIA
                     IGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIYKRVDSIAKVISICENSSFE
                     KFVNLFN"
     gene            1369..2286
                     /gene="pfkB"
                     /locus_tag="KPH93_003315"
     CDS             1369..2286
                     /gene="pfkB"
                     /locus_tag="KPH93_003315"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG1690087.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVNEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKKLKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDILYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNNELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            2323..4242
                     /locus_tag="KPH93_003316"
     CDS             2323..4242
                     /locus_tag="KPH93_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1690088.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKQEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVDMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YAINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMIGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1907 a          461 c          862 g         1495 t
ORIGIN      
        1 tattaaatga aaatagattg gtattaactg acatagaatt taagagtgtg ttaaataata
       61 tattagttat gttaagcaga cataagtata atgaaaaaga atttatagag aattatatag
      121 aaaattatag gagcaagaga agctcaattt tagaaaatga acaaaacaaa gaagttataa
      181 taaattcaat aaaaacattt tgtaaaaatt taaaattagc tacatcaata gatataagta
      241 atgataaggt atttgaagag tttttatata agcatattag tagtttatgt aaaaaaataa
      301 agttaggtat aaatcaatca cctataatgg ctcaagatat aaaaataaaa tatccatttg
      361 cttttgaact agctaatata gctaaaaaga caatagaaaa agatttgaag atagaaatta
      421 atgaagatga agttgcaaac atagctctac atataggagg agcagttgaa agagcatctt
      481 ataataataa agataaggtt ttcaaaacta taatagtctg tacttcaggc ataggcacat
      541 caatgttaat aaaagctaaa ttagagaata tatttaagga aaaattagaa atactaaaga
      601 ttattccttc atacttagtt gaatatataa aagttataga tgttgatttt gtaatatcta
      661 ctgttcctat tgaagtagga gatgtgccag taataaatat atctccaatt ttaagtgaga
      721 aagaaattaa attaattgaa aaatatatag aaacaggaaa tatataccta gatttagata
      781 taaaaagcct gtttgatagt gaattatttt ttactgattt ggtgtttaaa aggaaagaag
      841 aagttataga ttttatggca agtaaacttg tacaaaaggg ctatatagat gaggatatga
      901 aaaaatctta ctttgagaga gaaaaaatag caacaacaga aatagggaat atggtaacta
      961 taccacatgg agctattgga aaaatttttg ataataaaat agcaattggt atattaaaga
     1021 agcctatttc ttgggaaata tcagatgttc gtttaataat aatgttagct cttgataaag
     1081 ataaaatatt agattatgaa gtaatatttt caaaaatata taaaagagta gattctatag
     1141 caaaagttat aagtatatgt gaaaatagta gttttgaaaa atttgtaaat ttatttaatt
     1201 agatgggtag tattgtatat acacctgtaa atttggataa tataaaacta aataaggtaa
     1261 aaacgataaa tttctctttt aattaagaga aattttttga ctttcaagga tattaatata
     1321 aagttaagat ataaacataa agcaaataaa taaaaaagag gtggcaatat gatttataca
     1381 gtgacattaa acccatcaat agattatata gttaagctag atgaactaaa gacaggaagt
     1441 acaaatcgtg taaatgaaga atacgtttat ccaggaggaa aaggtataaa tgtatctcgc
     1501 atacttaagg aacttggaaa tgataataca tctttaggtt ttataagtgg ctttactgga
     1561 gaatatataa taagaacatt ggaagaaaaa aaattgaaga cagattttat aaagataaaa
     1621 agtggttttt caagaataaa tgtaaaaata aaggcattaa aggaaacaga gattaatgga
     1681 caaggtccaa atatagatga tgaagacatt gatattttat ataaaaaact tgataagcta
     1741 aatcaagatg atattttaat attggcagga agtataccat ctactttaga tgaaaaacta
     1801 tacgaaaata taatggctag attagaaaaa aagaatataa aagttgtagt agatgcaact
     1861 aaaaatcttc ttcttaatgt tttaaaatat aaaccttttc taattaagcc taataacaac
     1921 gaattagaag aactatttgg tgttaaatta aattcaattg aagacatggt aaaatatgct
     1981 aaaaggttaa aggaaatggg agccataaat gtcttagtat caatgggcaa agacggagca
     2041 ttgttgatta cagaggaaga tgaagtgctt ataagtgatg ttcctaaagg aaaagtaaaa
     2101 aattctgtag gagcaggtga ttcaatggta gcaggattta tatctgggta tttgaatact
     2161 gggaaatatg attatgctct taaattggga gcagcttcag gtagtgcaac tgctttttca
     2221 tatgacttag ccaaaagaga atacattgat aaattggtaa atgaaatatc tgtaaagcag
     2281 ttttaaaata ttaaaaaata tatagaaagg taattaatat ttatgaaaat tatagattta
     2341 ttagatgaaa agtcaattaa gttaaattta aattcaaaaa ccaaacaaga agcaatagaa
     2401 gaattagtag atttggttgc aaacagtggg aatttaaatg ataaagaaaa ctacaaaaaa
     2461 gcaattttgg ctcgtgaaga aatgagtaca acaggaattg gagaaggtgt agctatacct
     2521 catgctaaaa attcttctgt tactaaagct tgtatagcag cagcagtgtc aaaagaaggt
     2581 atagattatg aatcgtttga cggaagttta tcaaatttat tttttatgat agctgcacca
     2641 gacggagcta acaatactca tcttgaagta ttatcaagac tatctacaat acttatggat
     2701 gaagacttta gaaataaact tataaattca agttctgaaa aagaatttaa ggaaataata
     2761 gataaaaagg aaagagaaaa attcagtgaa gaatatcaag atgagaaagt tgctgaaaaa
     2821 aatataatag atactaaaga gaatgatgct aataagtatc ctaaggtttt agcagtaaca
     2881 gcatgtccaa ctggaatagc acatacattt atggcagctg aaagtttaaa taaaatggcc
     2941 gaaaataaag gtgtatcaat aaaagtagaa actaatggtt cagcaggtgt taaaaataag
     3001 cttacaaaag atgaaataga aaatgcaaca tgtataatag tggcagcaga taaaaatgtt
     3061 gatatggcta gatttaatgg caaaaaagtc ataaaaacta aggtggctga tggcatacac
     3121 aaagcggaag aacttataga caaggctgta aatggagatg ctccaattta ccatggagga
     3181 gatggaagtc acaacgaatc aaatgaagaa agtgaaagtg gatttagaaa agtatacaaa
     3241 cacttaatga atggtgtatc taatatgtta ccatttgtta ttggtggagg gatacttata
     3301 gcgatagcct ttttacttga tgattatgct attaaccctt caaactttgg ttcaaataca
     3361 ccgatagcag cattttttaa aggaatagga gataaagctt ttggatttat gttacccgta
     3421 cttgctggat atatagcata ctctatatct gatagaccag catttgttgt aggatttgta
     3481 ggaggtgctc tagcaggtga tggtggttca ggctttttag gtgcactact tgcaggtttt
     3541 atagcaggtt atttagtaga agggcttaag aaaatattta gtgtactacc agcttcattg
     3601 gaaggaataa aaccagtatt gttataccct ttacttggaa cacttttgat gggaattata
     3661 atgacatttt tagtaatacc tccagttaca gcaattaata atgcaatgat tggattttta
     3721 aatggtttag gtggaacttc aaaaatagtt ttaggattag tattaggtgg aatgatggct
     3781 gtggatatgg gtggacctgt aaataaagca gcatatgtat ttggagttgc atcacttgaa
     3841 tcagggcagt ttgaaataat ggcagcagta atggctggtg gtatggttcc gccacttgct
     3901 atagctcttg caactacatt ttttaagaat agatttacaa aagaagaaag agattctggt
     3961 aaagttaatt atgtaatggg attatcattt gttactgaag gagctatacc ttttgcagct
     4021 ggagacccac ttcatgttat accagcttgt gttggaggtt cagcagtggc aggagcactt
     4081 agtatgttgt tcaatgcagc tcttcgtgca cctcatggag gagtatttgt tataccagtg
     4141 gttacccatc catttgcata tatattagct atagctgtag gtgctttggt tggaatgatg
     4201 cttttagcat tacttaaaaa accattaaat caagaagtat agtaattaaa ggatattcaa
     4261 gaacttaaaa tatgatgttt tataaaaatt aatatattgc tgaattattt taaaataaga
     4321 attttatgag gctgttatgt aacgatttgt aattgttatc ttgacagtct tttattttta
     4381 tataagtaaa ataagtgtgt cttgcttgat ttatatttaa attgttttta agttagaata
     4441 taaagaaaat tatatattat tttaatatgc gttcgaacta gaaaataata tttgttgtat
     4501 tttttgtata taaaaatata ttgtaatatt aaaaaaataa tagtaaactg attgcataaa
     4561 aatgattttt atataaattt taaaaagaag aataaatttc taacgaaatt ggatatttct
     4621 tctgtaaata tatattcaaa aaaagaatta taagagttta gtcagtagta gtgtgagtaa
     4681 taaggacttt ataaatacat agaaatataa tgatattaat atagc
//
LOCUS       DAEMAE010000253         4617 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEMAE010000253 DAEMAE010000000
VERSION     DAEMAE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.256"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            125..2536
                     /locus_tag="KPH93_003317"
     CDS             125..2536
                     /locus_tag="KPH93_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG1690089.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKSGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            2833..4410
                     /locus_tag="KPH93_003318"
     CDS             2833..4410
                     /locus_tag="KPH93_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG1690090.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERLSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTITVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         1870 a          553 c          848 g         1346 t
ORIGIN      
        1 aaaaataaaa ctataatatt ctataattaa tatgtactca taatccatat tttatcaaaa
       61 taaaatatga ccttaattta gaaatctgat ataataaaaa ttaaatacat ataagggggt
      121 aaacatgaga aagtacaaat caaaaaaatt gtcaaagcta ctagcattgt tgacagtttg
      181 ctttttaata gtgtcaacaa tacctgtctc agcagaaaac cataaaactc tagatggagt
      241 agaaactgca gagtattctg aaagctatct tcaataccta gaagatgtca aaagtggaga
      301 cacagcaaaa tataatggag taataccttt tccacatgaa atggaaggta caacacttcg
      361 aaataagggt agaagtagtc ttccatcagc atataaatca agtgtagcat acaacccaat
      421 gaatcttggt cttactacac cagcaaaaaa tcaaggaagt cttaatacat gctggtcttt
      481 ttcaggtatg tcaactttag aagcatatct taaattaaaa ggatatggaa cgtatgactt
      541 atcagaagaa catttaagat ggtgggcaac tggtggaaaa tatggatgga acttagatga
      601 tatgtcaggg agttcaaatg taacagctat agggtatctg acagcatggg caggtcctaa
      661 gttagaaaag gacataccat ataatcttaa atctgaggca caaggtgcaa ctaaaccttc
      721 aaatatggat actgcaccta ctcaatttaa tgtaacagat gttgttcgtc ttaataaaga
      781 taaggaaact gtaaaaaatg ctataatgca atatggttca gtgacatcag gttatgcaca
      841 ttattcaaca tattttaata aagatgaaac agcatataac tgtacaaata agagagctcc
      901 attaaatcac gctgtagcga tagtaggatg ggatgataat tattcaaaag ataactttgc
      961 atctgatgta aaaccagaat caaatggagc atggttagta aaaagcagtt ggggagagtt
     1021 caattctatg aaaggattct tctggatttc ttatgaagat aagactctac taacagatac
     1081 agataactac gcaatgaaat cagtatcaaa accagatagt gataaaaaaa tgtaccaact
     1141 tgaatatgct ggtcttagta agataatgtc aaataaagta acagcagcaa atgtatttga
     1201 ttttagcaga gattctgaaa aacttgattc tgttatgttt gaaacagact ctgtaggagc
     1261 aaaatatgaa gtatattatg caccagtagt aaatggagtt cctcaaaaca actcaatgac
     1321 aaaacttgca agtggaacag tatcatattc tggatacata aatgtaccta ctaattctta
     1381 cagcttacca aaaggtaaag gagcaatagt agtagttata gacaacacag caaatcctaa
     1441 tagagaaaaa tcaactttag catatgaaac tgatatagat ggatactatc tatatgaggc
     1501 taaagcaaac ttaggtgaaa gctatatact tcaaaacaat aagtttgaag atataaatac
     1561 atatagtgaa ttttctcctt gtaactttgt tataaaagct ataacaaaaa catcttctgg
     1621 acaagctact tcaggagaat ctttaactgg agcagataga tatgaaacag cagttaaagt
     1681 tagtcaaaaa ggatggactt cttcacaaaa tgcagtatta gtaaatggag acgcaatagt
     1741 agatgcttta acagctacac catttacagc agcaatcgac tctccaattc ttttaacagg
     1801 aaaagataac ttagattcaa aaactaaggc agagttacaa agattaggaa ctaaaaaagt
     1861 ttatctaata ggtggagaaa attctttaag caagaatgta caaactcaac ttagtaatat
     1921 gggtatatca gtagaaagaa tttcaggtag tgatagatac aagaccagta tatctctagc
     1981 tcaaaagtta aatagtataa aatctgtttc acaagttgca gtggcaaatg gtgtaaatgg
     2041 acttgcagat gcaataagtg ttggtgcagc agctgctgat aacaatatgc caataatact
     2101 tactaatgaa aagagtgagt tgcaaggtgc tgatgagttt ttaaattcat caaaaataac
     2161 taagtcttat ataattggtg gaacagctac tttatcatca aatttagaaa gtaagctttc
     2221 aaatccaaca agacttgcag gaagtaatag aaatgaaact aatgctaaga taatagataa
     2281 attctaccct agctcagatt tgaaatatgc ttttgttgtt aaagatggtt caaagagtca
     2341 aggagacttg atagatggat tagcagtagg agcattaggt gctaaaactg attcgccagt
     2401 agttctagtt ggaaataagt tagatgaaag tcaaaaaaat gtacttaagt ctaagaaaat
     2461 agaaactcct attagagttg gtggaaatgg aaatgaaagt gcttttaatg aactaaatac
     2521 tcttttagga aaatagtgaa tggaaagata gtaaggatat acattttatt attaaaaaat
     2581 attcaataga agtttataaa tataaaataa caaaaagggg tgtctcaaaa tgaacttttt
     2641 agttcgtgag gcactctttt ttgttatttt atatttttat catttaaaga atgaaaaata
     2701 ggcttgtcta tacttttaga tagttctctt attattgatg aaaaatgtaa taattttaaa
     2761 ctgtaaaata gtgtaaagtt attttataat ataaatatag taagatttta aacaaaataa
     2821 gggggaataa taatgaaagc accaaaaact attttaacaa tactgacaat agcgcttact
     2881 ttaagtagca tttctataat accatcatat gcacttacag aggaaaaatt aataggtaat
     2941 ggtagatatg aaactgctgt aaagataagt caaaaggcat acagttctag ttctaatgta
     3001 gtgctagtta atgacaattc attagctgat gcactttcag ctacaccatt tgcaaaggct
     3061 aaaggagcac caatactttt gacagaaagt gataagctag atgatagaac tgaaaaagag
     3121 ataaaacgtc taggagctaa agatatttat ttaataggag gtactgctgt acttaataaa
     3181 gatatagaga acaagttaaa aggtaatgga cttaatgtag aaagaattaa tggtaaggat
     3241 agatatgaaa cttcactgat tttggctaat aagcttaagg atataaaaga tattaaggaa
     3301 gttgctgtag taaatggtga aaaaggattg agtgatgctg ttagcgttgg ggcaccagca
     3361 gctcaaaata agatgcctat aattttgtct agtcctaagg atggagtaga agcatttgat
     3421 aaatttataa gagatgagaa agttataaaa gcatatgtaa ttggaggtac aacttcagtt
     3481 tcaagagctg tagaaaagag tcttcctaat gcagaaagat tgagtgggaa agatagaaat
     3541 gagactaatg ctaaggtcat agaaaagttt tatactgata caaatttaag taatttatat
     3601 gtaactaagg atggtagtaa aaatgagaat caattaatag actcgttagc tgtcggagta
     3661 ttggctgcaa aaaatgagtc tcctgttgtt ttggttggta ataaattaaa tacaaaacaa
     3721 agagatatac ttagtactaa aaaattaaat actataacac aggtaggtgg aaatggaaat
     3781 gaagaagctt ttgatgaaat taaaagccta caagaaaaaa ctgtgtttga agcaaaaact
     3841 gttgaagaat taactgatat gataaatata gcaagtccta atgatattat aaattttaaa
     3901 ccaaaggaga atactgtaaa tgaagctttt agaatggtaa caaataaacc aataactgtg
     3961 aatataaagg gagattgttc aaagactatt acagtagata tgcctaatgg tgaagtgaat
     4021 aactatgcta ctttggtaaa tgttatagtt agaaatatcg gagaaggtgg atttaataac
     4081 catgatacaa tcactatatt gtcagttcgt gacaaaaatg gtagagttat agaaaatact
     4141 agaaattcag atatagatac gttgatgata ttagcttctg ctaatgatac aaaattgatt
     4201 aatgatggat atataggaaa acttatagat aattcatcta atagtgatat aactaataat
     4261 ggtactatag ataaaaaagt taatcaggtt gaggatttag aggctaaagt agattcaatt
     4321 gaaaaagcta tagattctat aagtcagaaa gtcaataaaa tacaagatat actagataag
     4381 cttggatttc taaagaaatt tcttagctaa gaatatgtat atttaagtga agtgtgtgtt
     4441 gtaattgttc taaagttata tagttaaaca agtttatata caaacagact tatagataaa
     4501 tatgtagttc aatatatatt tgaataagct ttggggaggt aacatgggga ttggaattga
     4561 atgtaacaaa aaaataaata aaagtattga caaaactaga ataaagaaaa ataatct
//
LOCUS       DAEMAE010000254         4608 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEMAE010000254 DAEMAE010000000
VERSION     DAEMAE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.257"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            498..1907
                     /locus_tag="KPH93_003319"
     CDS             498..1907
                     /locus_tag="KPH93_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG1690091.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKDKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTNITIPEVKLPLGISFFTFQIMSYVIDVSRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEHREHSWDKFTKGVNRFVVGLGKKVILANQLGIIADGVFNKSAGDLS
                     RLEAWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     NAFNVFIIEYYYVIVGAIILSTPIVKVVKSLLIKKNNNTLNSTLYQVTNAFFIISCMF
                     IVIVMLSSSTYNPFLYFRF"
     gene            2135..3115
                     /locus_tag="KPH93_003320"
     CDS             2135..3115
                     /locus_tag="KPH93_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690092.1"
                     /translation="MISINTKTQASLNKTKVGNYYLGKDNWILGMFSEILDQKQLNRY
                     EKSINYLSNLSKDMGKDVYFTMMPHKTNMLKHLYPKYVDNMDNIDINLKNFKNRLNPD
                     LIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMASNMDLSLSKQDLDK
                     HFSSYKKSVITDKKFIGSYNQNIDMLIKENEYVVYMTKGNQDYSFDINGEEKEEKEVY
                     ATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRDSYQAPTTLLLADMFKE
                     VEIADPRNINSIDKTYKEMIEESNADIVMFMYNSFGFAQMLDAMIDKGIK"
     gene            3315..4298
                     /locus_tag="KPH93_003321"
     CDS             3315..4298
                     /locus_tag="KPH93_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690093.1"
                     /translation="MKEVFNAHITQKQNKKIKQKYYLTKDGWLLGKFAKLLPDYHIKS
                     YSKAINNLSNLNSEKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDTDDYFYRSFTEKERENFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDNHLK
                     RYSTRYVNDKIFIGSYNKKMGYPLKKKESISYVEISDAKYKYFISNGTKNTEINENLI
                     KSKLKDKKEWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
BASE COUNT         1834 a          511 c          675 g         1588 t
ORIGIN      
        1 ttcaacaaat agtaactttt ttacaaaaaa tggaaactat ggaagcaata attaaaataa
       61 aatttataat caatcataat tattcagtat aataattcga tattatttta aagtatttgt
      121 catttaaaac aaacttgtga taaaagcata tctgaaagta ttctgattct aatacttaat
      181 aaagagttct ttttacagtt atagatataa acaacatcta tcttaataga ctgtatagag
      241 gcacaatcaa aggtaaacac atatagttaa gtaatatatc cttgattttt ctttattata
      301 atagctttat taattcattt taatattaca tcattttaaa aaaattatat gaaaagtcaa
      361 aatttacata tagattaatt aaacttgtaa aaacacatct aaattcatac aattaagtac
      421 ttaaaacttg aaaatactta ctttgctata tattttagat aaagatataa ttgatttggt
      481 taagaaagga gtctaatatg atattcagta gtttaatatt tttattttta ttctttccag
      541 tcacactcat ttgctattac ttggcaaatc ctaagtataa aaatgttatg ttacttctag
      601 caagtattgc attctacgca tggggagaac caaaatatat ttttttgatg atactatcaa
      661 taatagtaaa ctatatattt ggtattaaag tttctggcag taaagataaa aaaatgtggg
      721 ttgtgctttc tgtgatttac aatatatcta ttcttgctgt atttaagtat agcaattttt
      781 ttatagataa tattaatttt ttattcaata caaatataac tatacctgag gttaaattac
      841 ctttaggtat ctcatttttc acattccaaa taatgagcta tgtaatagat gtatctagaa
      901 atgatgctaa agttcaaaga aaattagcta atttagcatt gtacataagt ttattccctc
      961 agcttgtagc aggtcctatt gttagatatc agactgtggc agatagcatt gagcatagag
     1021 aacatagttg ggacaaattt actaaaggcg taaacagatt tgttgttggt cttggaaaaa
     1081 aagtaattct ggcaaatcaa ttaggtataa tcgcagatgg tgtatttaac aagtcagctg
     1141 gagacttatc aagacttgag gcgtggctag gtatagtttg ctatacactt caaatattct
     1201 atgactttag tggctatagt gatatggcca ttggtttagg taagatgttt ggatttgagt
     1261 ttttagaaaa tttcaactat ccatatatct ctcaaacagt atctgaattt tggagaagat
     1321 ggcatatatc tttaggtact tggtttaaag actatgtgta cttcccatta ggtggaagta
     1381 gagtctctaa ggcaaaacta tatagaaatc tatttgtagt atggttttta accggcatgt
     1441 ggcatggagc tagttggact tttattatat ggggtctata ttatggaata ttaatatcag
     1501 tagaaaaagc attcttagaa aagtttctat ataagatgcc aagaattgta agacacatat
     1561 atctacttct tatagtaatg attggatggg tattttttag agttgatgat ttaccagcag
     1621 ctattaaatt tataggtatt atgtttggtg ctggaaatta tccagttatc agtaatgcat
     1681 tcaatgtatt tataattgaa tattattatg taattgttgg agcaatcatt ctatcaactc
     1741 ctattgtaaa agttgtaaaa tcattattaa ttaagaaaaa taacaacaca ttaaatagca
     1801 cattatatca agttactaat gcgtttttca ttatttcttg tatgtttatt gtaatagtaa
     1861 tgttaagtag ctcaacctat aatccattct tatattttag attctaattt ggaggtataa
     1921 acatgagtaa aaaaaaacaa ggattgttgc catttcattt ttaattatag tatttggatt
     1981 tttcatacta aacttcacac ttccaaagaa aaaattaagt ataactgaaa atagaaatct
     2041 tgcaaaattt cctactgtag aaaatttaaa aaaaggtgac tttatagcca agtttgaaga
     2101 atattttaat gaccattttg catttagaga tgaaatgata tcaataaata caaagactca
     2161 ggctagttta aataaaacta aggtaggaaa ctactatcta ggaaaagata actggatatt
     2221 aggtatgttt tcagaaattt tagaccaaaa acaattaaac cgatacgaaa agtccattaa
     2281 ttatttatct aatctaagta aagatatggg aaaagatgtg tattttacaa tgatgccaca
     2341 taaaactaat atgttaaaac atttgtatcc taaatatgtt gataatatgg ataatataga
     2401 cataaattta aagaatttta aaaatagatt aaatccagat ttaattaaat atatagacat
     2461 ggatgagtat tttttaagta actattcaga taaagaaaga gaaaatttat attttaaaac
     2521 tgaccatcat tggactggat atggagcatt tgaaggcttt aaaatgatgg catcaaatat
     2581 ggacttaagt ctatctaagc aagatttaga caagcatttt agtagctata aaaagtctgt
     2641 aattactgat aagaaattta taggaagtta taatcaaaat atagatatgc taattaaaga
     2701 aaatgaatat gtagtttata tgacaaaagg taaccaagat tatagctttg atataaatgg
     2761 agaagaaaaa gaagaaaaag aagtttatgc aactgaaaga gataataaag agtgggatta
     2821 tggtggtgca tatattagag gagctaaaac taatatatta actataaaaa accctaaagc
     2881 attagtagat aaaaaaatac taatatttag agattcatat caagctccta caacacttct
     2941 acttgctgat atgtttaaag aagtagagat agcagaccct agaaatataa actcaataga
     3001 taaaacatac aaagaaatga ttgaggaaag taacgcagat attgtaatgt ttatgtataa
     3061 tagttttggg tttgcccaaa tgttagatgc aatgatagat aagggaataa agtagcaata
     3121 aaaagcctct attaaacttt aagtttaata gaggtttttt attgtttatt ttttattact
     3181 ttggtaaaaa taaactaatc aaaataaaag caaggagatg tagcatattg aataaaagat
     3241 ataacctact tataatatca gttatattat caatagcttt aattacattt aataataaac
     3301 agaataatat tacaatgaaa gaagttttta atgcccatat cactcagaag caaaataaaa
     3361 agattaaaca aaaatattat ctaactaaag atggatggct attaggcaaa tttgctaaat
     3421 tattacctga ttatcatata aagtcttact ccaaagccat taataacttg agtaacttaa
     3481 atagtgaaaa agatttttac tttgtttcac ttcctcataa gacaaatatg cttaaacact
     3541 tgtatccaag tggtatagaa ggtaaaaata atatagatat taacaaagaa aacttagaaa
     3601 agcaattaac agatataaat ttcttagata cagatgatta cttttataga agttttacag
     3661 aaaaagagag agaaaacttc tttttaaaaa cagaccatca cggaaattcg ttaggagcat
     3721 ttgaagaata taagtttatt cttgataaat tagaccttgg attatctaaa tcagaaatag
     3781 ataatcattt aaaaagatac agtactcgat atgtaaatga caaaatcttt ataggtagtt
     3841 ataacaaaaa aatgggatat ccacttaaga aaaaagagtc tataagttat gttgaaataa
     3901 gtgatgcaaa gtacaaatat tttattagta atggtactaa aaatactgag attaatgaaa
     3961 atctcataaa atcaaaatta aaagataaaa aagaatggga ttatggtgga gcttatatga
     4021 gaggttgtga ttgtcacata cttaagatta gaaattcaag ctctttaact aataaaaagg
     4081 ctcttatatt tagagattcg taccaagcac ctatgacttg gatgttggct gatactttta
     4141 aggaacttga aatactagat cctagatata taaaaaatat agatatgacc tatgatgaga
     4201 taattaaaaa cagtaatgcg aatgtagttt tatttatgta taatagcttt ggatttgctg
     4261 gaatgataca agaaatgatt gacaagggaa tagcataaca atttgaatca actccttttt
     4321 atacaaaaaa tagttctcaa taattcctaa tctatatgat aatttagtac atctctttaa
     4381 caataaaaat aaaatcacaa aaattcttat accatcactt atccttatga taaattggtt
     4441 gagaaaaaat tttataattt atatgatatg caatcttaat taattcttta ttttttaatt
     4501 ctttatctac tgcaaccttt gataaaagaa accatccaag actattaata tgaatttata
     4561 taaaaattac tttcaaataa agtactgcag tatttttatt taaagtat
//
LOCUS       DAEMAE010000255         4550 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEMAE010000255 DAEMAE010000000
VERSION     DAEMAE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.259"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            176..889
                     /locus_tag="KPH93_003322"
     CDS             176..889
                     /locus_tag="KPH93_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG1690094.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLINRDYKVDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKENTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQSLKFYLDS
                     PVSNSGNLKYGILEYAKIWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKDCNIVNLNK"
     gene            1005..1874
                     /locus_tag="KPH93_003323"
     CDS             1005..1874
                     /locus_tag="KPH93_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1690095.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLRENKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKTGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2029..3174)
                     /locus_tag="KPH93_003324"
     CDS             complement(2029..3174)
                     /locus_tag="KPH93_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1690096.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEIKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(3507..4283)
                     /locus_tag="KPH93_003325"
     CDS             complement(3507..4283)
                     /locus_tag="KPH93_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG1690097.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADAINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         1671 a          572 c          708 g         1599 t
ORIGIN      
        1 ataatataga atctatgatt ctgtattatt aagtggggtt aagcgttata attaattata
       61 aatattgaat aatctaaatg gataagataa tggtattaaa aattatccta aaaggtgaaa
      121 taatctgtat ttaatgtagt acagttatat atttaaagca aggtggtgaa atgatataaa
      181 taagatatct aaaagagggt ttgatgatag tgataaaaga tggttttcaa gtaaagaatt
      241 aacaagactt ataaaggcta aagaagaaat agaatggcta atcaatagag attacaaggt
      301 agacccagtc gttacttttg ttggtgatag atatcagttt tctataagac aaagagatgc
      361 attaaaaagg gcagtctgta ctgatgaaaa aaatattata agacaatcaa agaggctttc
      421 tttagataaa atcaaagaaa ataccgtaaa tatagatggc tttaatttga taatatccat
      481 agaggtagca ttatctggtg gtactctagt aataggaagt gatggcaata taagagattt
      541 agcaggtctt agaggaactt ataagattat agataaaaca gaagaagctt tgaatttgat
      601 aggtaaattc tttaataaat acaatgcaca gagtcttaaa ttttacctag attctccagt
      661 ttctaattca ggaaatttaa agtatgggat attagagtac gctaaaattt ggggaattga
      721 aactgaagta gaattagtaa aaaatgcaga tgttgtattg gaaaaattag atagagtagt
      781 tagtagtgat gcagttatag ttgataagtg tataagttat tttaatgtgg ctagaggtat
      841 aattgaagag tatattaaag actgcaatat cgtaaattta aataagtaga taaatagaaa
      901 tgagaatata agtatatata gcaatattat tagtaataca agtaaagttt gaaaatagga
      961 gaacgaatac atatcgaatt tcataaaatg ttgtaaaaag acatatgaga ggatggatat
     1021 gtgtgaaatt aactaaacta aatattaaaa aatatagagc acctatatat aaatatgctt
     1081 tagcaaatgt aaatttgagg tctgctaaat ctactaattc aagtatcata acagtaatac
     1141 ctcagggagc taaaatggaa gtattggatg aagaagatga ctggattaag gtgatgtata
     1201 attctcaaga aggatatgtg tataaggatt tggtatcagt aagcgaatat gcttggagta
     1261 acttaaactt aagagaaaat aaatctacaa cttccaatat aattacagta attcctgaaa
     1321 aatctagggt tgaagtcctt caagtagatg gagattggag taaggttgta tatgatgata
     1381 aaacaggata tgtttttaat tattttttat caactgatgg aaataaacca aatgaattag
     1441 attataagta tttttatact gacatgatta aatttgttaa cgaaaataat attaaaagta
     1501 ctagtgatta tctaattgta acagacctta gaaataaata tacttatatt tttaagaaag
     1561 ataatggagg ctggggacaa ctttacaagt ggcaatgtac tataggtaag cctgagactc
     1621 caactataac aggtatattt tatataagtg gaagaaaacc ttcttttgga acagatgagt
     1681 attcagttaa gtatgctact agaattaaag gtggatatta ctatcattct gtgctatatg
     1741 attcaacagg aagttatata atagatggca gacttggtga agctcttagc catggatgta
     1801 taagattaag tacggaaaat gcaaagtgga tatacgataa tataccagat acaacaacag
     1861 ttataattca ttaagaaaaa ttttaagttt tttattgaga taatgattct taaattaata
     1921 ggattatata ttaacttctc ataaagagta gtatttaaaa agtgtaataa gagagtgtta
     1981 ttaacaaaat aaaggtattt tgaggcactc tcttataaat atacataatt aagatatttt
     2041 cataaacaat tctataatct tctctttatc aaagaaaaca ggattagcac cagcacaagc
     2101 atctttcata gagttttctg caagcaattc aaaattagga ttatcaacac ctacatcttt
     2161 taaagatttt gggattccaa cctcttcaga aagagctttt atcttatcta ttacaaagtc
     2221 aacacactct ttatctgatt tattatttac atccattcca ataacttcag ctattggtcg
     2281 gaatttggca ggggcagact tagcgttttc ctcctcaact attggaagta gcatggcatt
     2341 acatactcca tgaggtaaat catataaacc tcctaattga tgagccatag catgtacatt
     2401 tccaagacct gcattactaa aggcaattcc atttaaaaaa caagcataac acatttgttc
     2461 tctagcttct atgtcattac catttttaac ggctcttgga agatatttaa atatctgctt
     2521 tatagcatat aaggcagttg aatctgtaac atcataagca ccattagcta ctacagcttc
     2581 tatagcatga gttagtgcat ccataccagt tgctgctgtt aaattagatg gtttactaat
     2641 cattaattct gggtcattta ctgtcattgt ggctagtgaa ttggtatcaa ccattatcat
     2701 ttttacatgt ctgtcttcat cagttataac atagtttatt gtaacttcag cagatgtacc
     2761 agcagtagta gttatagcta taataggaag acatttttta gaagttttgt tgataccttc
     2821 atagtctcta atatctcctc cattagtagc caatatagag atagctttac cacagtcttg
     2881 tggagaacct cctcctatag tgatgacgaa gtcgcaattt tctgacttta atattttaag
     2941 tcctttattc acattagtta cagttggatt ttgtttaatt tcatcataaa caacaaagtc
     3001 tacatctatc tgagataaaa tatctgttac tttttttaca gtaccatttg aaactagaaa
     3061 tttatcacta acaacaaatg cttttttagt tcctaatgat ttaattgaat ctttagcttc
     3121 attaagacat cctttcccaa gtaaatttat tggtggtaca taataaatgc tcataactaa
     3181 aatctccttt tattttcatt aatttttatg taaaaatata tatctaacaa ttttcactaa
     3241 tttactcata atatatcgta tcttagattg atatagagtc agtagttctt atataagaac
     3301 ttaattcgta attacgatta tgattagaat ttaacatagt atttttatca tgtcaataaa
     3361 aatataaaaa tattgtaaaa aaaaacaaag ttactgtagt taaatcagca actttgtttt
     3421 tatagtataa atcaaataaa tacattagtt taaaacaaaa tttttaatta ttatgttttg
     3481 atatcttcca tatacagaat attaatctaa tttattacaa tgtcttcatt acgttcttcc
     3541 atcattgatt gaatattttt ctgagcattt aagagtaatt tttttatcag ttctattttt
     3601 tcatcattag ctctaaataa aggcagtgtt acactaacag cagctcttat acatccattt
     3661 tttcttattg gtacggcaag acattttagg tgttccattg attcctcaca ctctgaagct
     3721 atttgtgttc tttgaacttc aagaagctgg tcgtataaaa catcaaaatc tgtaattgat
     3781 ttttctgtaa aaccttttag accatctgga tataagctta ttagtttttc cttgtcaaaa
     3841 tcacataaaa gagcttttcc aagacctgta caattagcag gtattttttt tccaacataa
     3901 gaaataagtc taattggttc tggagaatct acttttccaa tataaagaac ttcattattg
     3961 tcaagtatac caagctgaca agtttcagaa caggaattaa ctatattcgt catttcgtca
     4021 attatatagt taaaaatagt tcttttttca aaaaatgaag accctaatgt gtaagaacgt
     4081 atccctatag aatatttgga tgtgttttta tcaatagtaa tgtattttct agcattaagt
     4141 gtatgaacta taggaaagat gctacttttt ggagcattga tagcatcggc tatttctgta
     4201 agagtatatc cattttcgga ttctgctaat gcttctaaaa tatctaatat cctagctgtt
     4261 gggcgatgat tatctgaagt cataaatttc cccctctttt tattaattta aagtattaga
     4321 atatgttgat tttagcacac tatacgatat tttacaatgt agaaaataaa tatgtaaatt
     4381 tatatataaa taagctcttg atttagatac agatagatat ttttttgggc tgtacattaa
     4441 ggaatagcta atttccaaaa tatacctaaa gaaagtaagt gatatttagt tatataattt
     4501 aagtaaatat atgaaatagt gatttttata tgtaaatatc aaaatattat
//
LOCUS       DAEMAE010000256         4531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEMAE010000256 DAEMAE010000000
VERSION     DAEMAE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.260"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(108..338)
                     /gene="dltC"
                     /locus_tag="KPH93_003326"
     CDS             complement(108..338)
                     /gene="dltC"
                     /locus_tag="KPH93_003326"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG1690098.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(357..1505)
                     /gene="dltB"
                     /locus_tag="KPH93_003327"
     CDS             complement(357..1505)
                     /gene="dltB"
                     /locus_tag="KPH93_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG1690099.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1505..3019)
                     /locus_tag="KPH93_003328"
     CDS             complement(1505..3019)
                     /locus_tag="KPH93_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG1690100.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERDFGDIDVIDMDKLKDIINEYQGKSLDKESWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPKLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3140..4330)
                     /gene="dltD"
                     /locus_tag="KPH93_003329"
     CDS             complement(3140..4330)
                     /gene="dltD"
                     /locus_tag="KPH93_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG1690101.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTLPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNEYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         1475 a          797 c          455 g         1804 t
ORIGIN      
        1 ttcaaaagac tttgtattat cattaaaaaa gtccatcaaa tattagtatc agatggactt
       61 tgtaaaattt tgttttataa attaatattt aaagtctaac tactaatcta ttttcttgtc
      121 tccaagaatt ttactaaatt atttacagta gacatatctt ttctctctaa atcagttggt
      181 tgaagctcta tccctaatct attttcaatt tcaagtaaaa cttcaattat agctaatgag
      241 tctaaaagtt ctgtctcaaa taaattaagg tctaaatctt ctcttatctc atcagtccct
      301 aatacatctt caaatatttc tataactgtt tcttgcataa attttcctcc ttaaaattaa
      361 gctaaataac ctgagaatat cagcaatcca aaacacacaa tatggaatgt aatgaaaatc
      421 tgcactctct cgaaccattt atctttttta tgttttttat aaaatttaga ttttcgctga
      481 tatatatcag ttaacacaag tgccaatcct tggtatacac cataagcaac ataataccaa
      541 gttagaccat gccaaaatcc cattgttatc attgttatca tctgagccac atgagcagca
      601 gtagtccttt tcttaaatct tttctttctc attgatgaca taacaaatct agaaaaaata
      661 taatctccaa accatcttga gagacttata tgccatcttg tccagaactc tttcatatct
      721 ttactaataa atggcttgtc aaagtttata ggtacttgta ttccaaatat gtaccctgta
      781 cctatagcaa ataaactata tcctgcaaaa tcaaagaaca ggtataaact atatgcgtac
      841 atataactta aaatatttat aaaactcata tctttaggta ttcttgatac ccaatatgta
      901 tttatcaaaa atgctataac aaacttataa cctactccca taacaatatt ttttattcct
      961 ggcagtaagt attcttctat atactctttt ctagagattt gtttttccaa gtcctgttca
     1021 aatcttcttg acctatcaat aggtccagaa cttaaagttg gaaaaaataa cataaaataa
     1081 agcatctttg aaattttaac ttcttttata gctccatcat aaatttcaat aaccatttgt
     1141 atagttctga aatttaagta tgatattcca ataaatccaa ttattccaaa agaagtaact
     1201 ggagatattt tatttattat tattgggagc attgatgcaa acaagaatcc ccaatatatg
     1261 tatttatttt tagtttttct tcttacatat tcatatcctt taactattat tacttcaaga
     1321 ataataaata ttaccaagta ctttagttgg acatcaattc ctactattaa gaatatcata
     1381 aacaaacttg ccaacatacc ataatacttt atattcttgc ccattaggcc taaaattact
     1441 gctggtattg atgttaataa taatatataa agataaaaat aatctccata ctgtgaaaaa
     1501 atcattaaat ttcctccata agtttctttc tgtctatttt accattgata tttgttggaa
     1561 actcagagat tattgaaata tttcttggaa tcatatatga aggtatatac tttccaagtt
     1621 ccttttttat tatcatccca tttttaatat tacttaaatc attcttttca tttaactcaa
     1681 caataccttt taaataagca attttttcgt ccttatatac tggtaaaact actgcatttt
     1741 ttacattatg tacttttctc aaattatttt ctatatcttc tatttcaatt ctaaatccat
     1801 ttaatttaat ctgaaaatct tttcttccac aataatatat gtttccatct aaaagatatc
     1861 ccatatctcc agttttataa gctctccact ttacaccatc tatttcatca tagaaaaata
     1921 cttcatcagt cttttcttta ttattgaagt aacctttaga aactgatggt ccaattatta
     1981 taatctcacc cttttcattt tctttaagtt catttccatc ttcatcaagt atttttattt
     2041 tacaatttga cataggatat ccaactggaa gacttttttc atcatctata gctttttgag
     2101 tcatatcatt tacacttaca ccaacagtag cctcggttgg tccatatcca ttgataattc
     2161 ttgtgtttgg aaatctactc ataagttctt tagtaagatt ttttgaaagt gcttctccta
     2221 tgaatatcat tgactcaagc ttaggaagca ttttactatt aaactctttt tcagttatac
     2281 atactcctgc aaaagaagga gttgacaccc atacagctat atctgagata ctaaattgtc
     2341 taaatagttc tttatagtct gctaatacat cttttgatat tgaaaataat gtagctccat
     2401 ggataagacc agggtatatt gttgtaactg aaaggtcaaa agaatatgct ggttggttca
     2461 ttattacttt ttcacttcca tctatgttta gatatgggct tatccaatct gaaaagctat
     2521 ctaaattgtt tgaacttatt tgaactcctt ttggttttcc tgtacttcct gatgtaaata
     2581 atatatatgc attttcgtca tcttttaccc agctttcttt atctaatgat tttccctgat
     2641 attcatttat tatatccttt aatttatcca tatcaattac atcgatatct ccaaaatctc
     2701 tttcatcact aaagttaaat attacttttg gttttatctc tttagtgact tcaaaaactc
     2761 tatcaattgg aaaactaata tcaagaggca catacgctct acctgatttt aaagctccta
     2821 tcatacatgc cattatcata ttttctttat ttccataaat tactatagga gtatcttctt
     2881 ctttgtatac atcctttaga aatactgata tagcatctga atattcattt aaatctttat
     2941 aagaaagttt atctccatta cacattagtg ctgttctatc tgtatttgaa tattttttta
     3001 ttccttcaat aattttcata taagaatgct cctttaaaat ccgttataaa taaatggcaa
     3061 ttctttaaat ggagtaaatg ttagaactcc tatcgccaat attataacca tagttaaaca
     3121 aatactagtt aacaaatctt tattgttctt taaatatttt aaataatctc tcacagacat
     3181 ctacccatcc tttcgtacca agatgcatta catcccttaa ataatactta tctgaccctt
     3241 tatctttaag atttagaact tcaaagcctt tgctttctgc tattttttgt gctttatcgt
     3301 agtactcatt tctttctttt tcagaaatac cagtcaaatt atagaattta tccatacttg
     3361 ggattaatac aacaactggc ttaataccta agtcagtaca tacatttagc atcagtttat
     3421 aattttcaaa ctccttagat gttaataaat ttacatcttt gtctcttcct ttgtattgct
     3481 ctataccatc tttaaaatgc tctttataat aatacttatc aatattcagt ggatttttac
     3541 caactctttt tttagcatct tcaatggctt tctttctctc atatgaccaa tttattggtc
     3601 tttttctttt tgttgctctc tttttatcta gcttaattaa tcgtttataa agaataccct
     3661 tttctttaag agctatacag tactttcttc cttgaaaata aggctctaaa agcacctttt
     3721 cagctttttc aggcaatgtt ttaggctcat atagcttcgc atataatgct tctgctttat
     3781 actcgtcact tccccataat agcttacttg atttttttgc atattcaatt ttattttgtt
     3841 tagatatttt tggattatct aaaaatcgat aaaattgtat tggtgaaaat ctagactgat
     3901 aatggtgact tgtgactccg tctttttcca taaaccattg catagaaatt aacaagacaa
     3961 ctttcttatt atcaatatta ggattcatac ttcctaatat tgcagcatct tgtaatgtct
     4021 gagtataggc tctcccaatt gcaaacacct tatttttact cctatttgta ttaaaatagt
     4081 actttggatg ttgttttgtt gaattactaa gttctgatga ccccaaaatc ataatatctt
     4141 tttctcttaa aaaatggtta tttgctgtaa cccctttatc ttttatatca cttaaagtat
     4201 catccataat tggaagtaga tttttctccc tcaaaagttc atcagtttta gaatcaagaa
     4261 atttgtctaa cccaaataaa aatactacgc ctattataaa aggtgtaata aagtatatca
     4321 attttctcat attttctctc ctctaaaaat attcaaattt atgttattat taatcatatt
     4381 agttttgata tgtatattca actacaaaat tgtaataaaa tcgtcaaaac tttttttgat
     4441 actgttacaa aattgtaaaa aaaagaaaaa aaagcaccaa aaataaattt ctttttaaca
     4501 ctttttcata cttataattt gacattttaa c
//
LOCUS       DAEMAE010000257         4501 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEMAE010000257 DAEMAE010000000
VERSION     DAEMAE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.261"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..306
                     /locus_tag="KPH93_003330"
     CDS             76..306
                     /locus_tag="KPH93_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG1690102.1"
                     /translation="MEIKKKLVMSFKNIKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLTSVVEAKVVETSTTPYDLVIE"
     gene            319..468
                     /locus_tag="KPH93_003331"
     CDS             319..468
                     /locus_tag="KPH93_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG1690103.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            725..1162
                     /locus_tag="KPH93_003332"
     CDS             725..1162
                     /locus_tag="KPH93_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG1690104.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDVLYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1418..2311
                     /locus_tag="KPH93_003333"
     CDS             1418..2311
                     /locus_tag="KPH93_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1690105.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWRHIAVLRPSQIAYMLFESFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGFACGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMTYYFFNVQLLLHYPIKLCVIAQYVQIIFSLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2489..3313
                     /locus_tag="KPH93_003334"
     CDS             2489..3313
                     /locus_tag="KPH93_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1690106.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3411..4019
                     /locus_tag="KPH93_003335"
     CDS             3411..4019
                     /locus_tag="KPH93_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1690107.1"
                     /translation="MEKLLRGKIMKADSTKEQLITATIQLLSSHKDISSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKHHMDKYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKVSVPYILIQEEIVYPFEILPLIKAHYENQNNKKTESEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            4123..4299
                     /locus_tag="KPH93_003336"
     CDS             4123..4299
                     /locus_tag="KPH93_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690108.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         1742 a          526 c          611 g         1622 t
ORIGIN      
        1 taatattgac aattaaaata ccaatattaa caatcaaaca attaaaatat aaattattat
       61 atcggaggta attatatgga aattaagaaa aaattagtca tgagttttaa aaatataaag
      121 gataaacaag tttctttttc tatagaaaat ccaaaggatg atttaactga agaaaatata
      181 aaatctgtca tggatttgat agtatctaaa aatatttttt cagttggagg atttgaccta
      241 acttctgttg ttgaagccaa ggttgtggaa acaagtacca ctccttatga cttagttata
      301 gaataggagg ctaattttat ggatgatagt ttacagctgt taataaccaa tgttggtttt
      361 cctatagctc tatccatgta cttgttggtt agaattgagg gtaaattaaa tactttatca
      421 aacagtattg atacattatc caaaaatatt ttgatattta agaagtaatt tgacaatata
      481 tgtaagtcat aaaataggtc tatttgtaaa ataattagat atattctatg cgaaatacta
      541 ggatatttgg ataatttatc caaatatcca tagtattttt ttgaatttta ccacctcaaa
      601 tttgaatttc actattttat ttttgaattt cactatgctt aatttgaaat ttagaacgca
      661 tctagttgga ctcaataaga tataatgcta ttctaatgac agtaataaaa tgaggtgatg
      721 attaatgaaa atacatgtcg aacaaggtat taattgtgat gatattgaaa taaccataaa
      781 atgttctgct ctagataata acattgaaaa aataatttca ttattgagta caccattact
      841 tgatataaaa ggtaaaaaat caggagagat ttataagctc aatgtctgtg atgtcttata
      901 cttcgagaca gttggaaata aaacatttgc ttattatgaa gatgatgtat ttgaaattga
      961 tttaaaaata tataaattat caaaattatt agctaataca tctttctcac gaatatccaa
     1021 atccatgatt ttaaatatgg actacataaa aagtataaaa gtaatatcac ataatcgcat
     1081 gcaagctact ttaaaaaaca acgaaaaagt tattataaat cgtcgttata taaaaaatat
     1141 taaagaaaag ctaaatattt aataaaaatc taaagtaatt tattaatatc actaatttca
     1201 aatttattga cctattcaat aattaaaata atataatatg ttcaatatta aatgctaatt
     1261 ggttatattt agtgtaaaaa taattaatca aaatataaaa aataggatat tactgtaatc
     1321 aataactaac atcaaacgat aacttaatac atatagaaat aaataaacaa ttaataaaaa
     1381 ttaaaacttc attataaaac ggaaggatag tgaaaaaatg caactatata acaccccatt
     1441 taatacaaac aaacacctaa tctatttctt tacatttact ctagtattga actggatatt
     1501 cagtcttaca cctgcattac tcgggattac aaatacgata cttggagaaa tcatttttta
     1561 tcttgcatat acatcaccag ctattgtagg tctatttttt atttctacaa tctatccaca
     1621 aagtgcaaaa caagatttta tttatagata cctaagcttt aaagaaatcg gaataaaatg
     1681 gatattatta acaatattat tttttgcttt aacttttatt acttctttaa taatcggaaa
     1741 ttactttaat gtaaaaactt ctgatataga ttggagacac atagcagtgc ttagaccttc
     1801 tcaaatagct tatatgctat ttgaaagttt tatattaagt atacttagtc aagaagctgg
     1861 atggagagga tacgccattg acaaattatt agttagattt ggatttatag gctcttcaat
     1921 tattttagga tttgcttgtg gaatctggta tctagggtcg tattttacac ctaatcaaat
     1981 accatataat ttggcacaat actcactatt tgatgctttt ttatttatac caaacattgt
     2041 acttttaaac tttataatta attttgtata tatcaataca agtagaagta ttttatcagc
     2101 tggattagtt catatgacgt actatttttt caacgttcaa ttgcttctac actaccctat
     2161 taaattatgt gtaatagctc aatatgtaca aataatattt agtttaatat ttcttgtata
     2221 tatgatttct tctaataaat ttaaacagaa ggtaaattct gaaattgaac agataaagtc
     2281 ggatgatttt gaatttgagt tagactggta attttaatat gaattataaa ttaagataat
     2341 ctatgatttg acacgattac tacttaaata taatttactc tctataattt attgcaacat
     2401 ctggaaaatt attgtatttc cacaaactct tgaagtatat aaaaacaaaa tatatactga
     2461 ttataagtta aaaggaggtc atattttcat gtttaaaata ggagaggttt caaaactaac
     2521 acaaatttct attcgtatgt tgcgttatta tgatgaactt ggtattttaa agcctgcaga
     2581 aatagacaaa tatacaggtc atagattata ctcagttgaa cagatttcaa tactacaaag
     2641 aattgtatta ttgagagatt caaaatttag cgttgcagaa atagcaaata tcgtacataa
     2701 ttggaatgat gaatttgtaa ttaaggaatt gaatagaaaa aagaatgaaa tacaaaagga
     2761 aataaagcag gaacaacaaa gaataaataa aattgataaa tttatagaag ctataaactg
     2821 tgataaagat gaaattcatt acaatgttgt atttaagaaa attccaagtt ataaaatcat
     2881 atcacttaga gagataatcc cagactacca atctgaaggt atactctggg aaaagttatc
     2941 taaatttatt aaagaagaac atatagaagt atcacgtcaa tccaacaata atatagcttt
     3001 ttaccatgat gaagaagtca aagataatgg tgtagatatt gaagttggaa tggttgtaaa
     3061 gaagattgga aagaacaaat caggatttat atatcgcgag acagaagaaa ttgatatgat
     3121 ggcatgtacc atggtgtatg gtccatatga aaatatagct ggtgcatatg aatcgttctg
     3181 ttattggtta gataaaaata gtgattatca aatatcaggt ataaatagac aaatcggtca
     3241 taagggtgaa cataatgaaa taaatcctga aaattatctt acagaaattc aaatccccct
     3301 tataaaagtc taatcgatta gtacttaaat acttaagtac taatcaccca ttgacatccc
     3361 aaaattttca tgatagtatc taaacgtgta ttaaacatgt ttaatttttt atggagaaac
     3421 ttttaagggg aaaaattatg aaagcagatt caacaaaaga acaattaatt actgccacaa
     3481 tccagttact atcttcacat aaagatattt caagcattac agctagagaa attgtagcta
     3541 aagcaaatgt caatttagct atgattaatt attattttaa atctaaagag gagttaatta
     3601 atatttctat taaaaaattt ttaaagcatc atatggataa atataaggct acacatgata
     3661 ctaaaaattt agcacctaaa cagcaattaa gggaaatact aattgcattt tttgatttta
     3721 taggtgaaca tagtcaattt ataaaggttt ctgtacccta tatattgata caagaagaaa
     3781 ttgtttatcc atttgagata ctacctttaa ttaaggctca ctatgaaaat cagaataaca
     3841 aaaaaactga atctgaatac agaactattg cgtatcaaat aacttcattt attcagttgc
     3901 tttttcttcg ttctaatgct tttttcaagt atagtggcat tgatattctt aatgttgagc
     3961 agagaaatgg cttaatagat tttcaattag atttattatt ggtagatatt acaaaataat
     4021 actatattaa ttttttatgt acattttttt attttatggt aacgcttgaa aatattctca
     4081 ttgactaaaa atatttgcat tagataaggg ggaggaattt taatgttttt tacatttata
     4141 ggagtaatat cagcaatttt attgttgtgt acaattatat gtggtttatg gataaaactt
     4201 aataatcgta ataatgactc taattctcac tttacttttt caatactttc aatcgcgttt
     4261 tcactgttga ttgtggtagc ctttttagta agaaattaat gtctttatta tgttggggtt
     4321 tattattata gatttttatt attgcatgat tttgtcatat tacatcattt ttattatatt
     4381 acagagtctt atttattaca ggatttttat tatattgtaa agacttattt attacagtgc
     4441 tttgttatat tacagacttt tatattacaa catttttatt atattacaag ctcttattta
     4501 t
//
LOCUS       DAEMAE010000258         4399 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEMAE010000258 DAEMAE010000000
VERSION     DAEMAE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.262"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(17..1408)
                     /locus_tag="KPH93_003337"
     CDS             complement(17..1408)
                     /locus_tag="KPH93_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG1690109.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSILGVIVLIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELKKISIDVDDLLTQADRVREMYKEENTTYDIAINYIN
                     TVSILNGIGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYNKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(1574..1822)
                     /locus_tag="KPH93_003338"
     CDS             complement(1574..1822)
                     /locus_tag="KPH93_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690110.1"
                     /translation="MMICFAMTFALLLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKPIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            2088..4217
                     /locus_tag="KPH93_003339"
     CDS             2088..4217
                     /locus_tag="KPH93_003339"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG1690111.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VNKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQENKTDIKNDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYKNYD
                     YTGEKNTKKIAMKTLKDSKKLIRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         1568 a          643 c          546 g         1642 t
ORIGIN      
        1 aatttaatat tattaattag tattcattta gtccattttc tgatatggca tatatttttt
       61 cattgtaaat attaatagca taaacttctt tgcctctaaa taagccttta tttactacag
      121 cataataata aatattttta tattttccaa acaattcttc taaactcttt ttacttttta
      181 catatctatc tgctttctct atatctaata tttcaatctt tttatactta ttttcttttt
      241 catcaagagt attagttaac acaaacttag ctttatcttt tgtgatacca aattgataac
      301 ttccatcttc taatattcta aagtatccat caccataatc tactttgtaa tcttttttac
      361 catctttgta tgaatataaa tctttagttt ttgcacttac caaaacttca tttattaatt
      421 tattatactc atatacttca gcataatagt atttattatt ttttatcatt tgtggaagtg
      481 aatttataga tagatttgcc ttgtctttgc cacttttttt agctattaca tttataatgt
      541 catcagatgt taaagtatac aatgataaat tctttttata ttttttttct aaactctgta
      601 ccttttcatc atctgaataa tcttctttta aatactcaat atattgtaat gctctttcat
      661 aatctccaga agtatttgcc tcttcagctc tacttatata gtaatcatac atttcttcaa
      721 tacattgttc tttcccattt tgagccatac tataatactt ttcatcttct tttaatactt
      781 tattatactt ttcaatagct tcataatact tatatgcttt ttggtctttt actgctgagt
      841 cataaacatc tctagactct ttgattgttt ctatattctg tttgtataca tctaggttac
      901 tacctatgcc atttaatatt gaaacagtat ttatgtagtt aatggcaata tcataagtag
      961 tattttcttc tttatacatt tctctaactc tatcagcttg agttaataag tcatccacat
     1021 ctatacttat tttttttaat tctttcacgg tatttattaa tccaataaaa ctttcttgac
     1081 ttatatctcc atctaaatat ttatcaccac tatttaatag taatttattt atttttttag
     1141 aaacagactt attaaacctt tccatttttg aatcagaaaa ctttttttct atacttgtgt
     1201 agtaatcaac agctatagaa tactgtcttt tctctataat ctttaacaac tcattagctt
     1261 catttcctac actaaactcg ttactactta agaaaaatac tattatcaaa acaattacac
     1321 ctaatataga tagtgaaatc gcaactatag atttcctttt ttcatcaata aaatctacag
     1381 ccttctcaaa taaatctcta aaattcatta tagttccctc tccatttaat aatctttata
     1441 ttttatggta aactaaattt cttattggtt attatatcat aattcttgaa aaaaaataaa
     1501 aacacaagtt aaatcgtgtt tttattttac ctatcaatca ttattttata cataaatatt
     1561 tcctttacat aaattatgat atctttcttt ttatatccct agaatgactc ttttgttttt
     1621 tgttagtact gacagttttc gttcttgttt tatgtatagg tttgctatca aatatttttc
     1681 tagcattgca taaaacaatt ccaattgtta aacaaactgt aagcacgctt cccatcaaaa
     1741 agtaataatc caatcctata tgtactccca taactgaatt tattaacaat aatgcaaaag
     1801 tcattgcaaa acatatcatc atggagataa ctaaaattct tagaattttt ttcaatccac
     1861 tcacctcgta taatcactaa aattgacact accccttatc cattactata ccaaattagt
     1921 aaaaaaatag caattactat tgtcatttat attaggaaaa tattaaatct ttttaataaa
     1981 tatctttttt tgacataagg attacttaac ttttaataaa gtagtaacac taatatgtat
     2041 taattaatag aataaaataa atgataattt attgaggggt gatttacatg ccaaatttgc
     2101 caagtttagg gtcaaaggct cctgatttta aagccaatac aacaaatggc cctattagac
     2161 tctctgacta taagggtaat tggattgttt tattttcaca tcctggtgat tttacaccag
     2221 tttgtactac agaattttta tgttttgcta aatattatga cgaatttaaa aaaagaaata
     2281 cagaactaat tggtctaagt gttgatagta acagttcaca tttagcttgg atgtataata
     2341 tttctttact tacaggtgta gaaattccat ttcctattat agaagataga gatatgagaa
     2401 ttgccaagtt atatggtatg atatcaaaac caatgagtga tacatcaact gttcgctctg
     2461 tatttattat agataataat caaattctaa gaacgattct ttattatcca ctaactacag
     2521 gaagaaatat tccagaaata cttagaatag tagatgcact tcagactagt gatagagata
     2581 atatagttac tcctgcaaac tggtttcctg gaatgccagt gattttacct tatcctaaaa
     2641 actataagga attaaaaaat agagttaaca gttgtaataa gaaatattcc tgtatggact
     2701 ggtacttatg ttttgtacca gataattata atgatgaaga agtgaacaag aaaattgata
     2761 atacttgtag ctggaaaaaa gaacatacta aaaacattga aaatgaatgt aactgtgaac
     2821 atgaacatca tgactacctg aacaaagctc ttgactgtaa acaagaaaac aaaactgata
     2881 ttaaaaatga ttgcaaccat gagaaaaaac atactaaaaa tactaacaag gttcacaact
     2941 ccaaacaaga taagtttaaa gataagtctt gtgatgaaat gaattttaac tatgacaaag
     3001 atgaatcttg tgacaaaata aattctagct atgacaaaga agatagtagc tatgaagatt
     3061 tctacaaaca taattataaa aactacgatt atactggtga aaaaaatact aaaaaaatag
     3121 ctatgaagac tttaaaagat tcaaaaaaat taattagacc acaaataaca gacccataca
     3181 atccaatagt tgaaaatgca aactgtccag atataaatcc aattgtagca gaatatgttc
     3241 ttggaaatcc aactaatgta gatgctcaac tattagatgc agttatattt gcttttgctg
     3301 agatagacca gtctggaaat ttgtttattc cttatcctag atttttaaat caattacttg
     3361 ctcttaaagg tgaaaaacct agcttaaaag taattgtcgc tattggaggt tggggagctg
     3421 aaggtttttc tgatgcagca ttaacgccta catctagata taattttgca agacaggtca
     3481 atcagatgat aaatgaatat gctttagatg gaatagatat agactgggaa tatcctggaa
     3541 gtagtgcatc tggaataaca tcaagacctc aagatagaga aaacttcaca ctcttactaa
     3601 ctgccataag agatgttata ggagatgata aatggcttag tgtagctgga acaggagata
     3661 gaggatatat aaattcaagt gctgaaatag ataaaatagc tcctataata gattatttta
     3721 atcttatgag ttatgatttt acagcaggtg aaacaggtcc aaatggtaga aaacatcaag
     3781 caaatctttt tgattcagac ttatctttgc caggatatag tgttgatgca atggtgagaa
     3841 atcttgagaa tgctggaatg ccttctgaaa aaatccttct tggtatacca ttttatggaa
     3901 gattaggtgc tactataaca agaacttatg atgaacttag aagggattat ataaataaaa
     3961 atggatatga atatagattt gataatactg ctcaagttcc gtatttagtt aaagatggag
     4021 attttgcaat gtcatatgat gatgctttat caatattctt aaaaactcaa tatgttctta
     4081 gaaattgtct aggtggtgta ttctcatgga cgtcaactta cgaccaagca aatatactgg
     4141 ctagaaccat gtctattggt ataaatgacc ctgaagtatt aaaagaagaa cttgaaggta
     4201 tttatgggca attctaatag aatttaggaa aattttatag ttatataaaa agcagatttt
     4261 attatattaa ataagttgta cctaattaat aatattatta attgtttata aattagatac
     4321 ttattttttc ataatacaat ctgctttttt aatcaaaatt tatttaatta caagttattt
     4381 aattataagt tgtttaatt
//
LOCUS       DAEMAE010000259         4345 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEMAE010000259 DAEMAE010000000
VERSION     DAEMAE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.263"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(320..1795)
                     /locus_tag="KPH93_003340"
     CDS             complement(320..1795)
                     /locus_tag="KPH93_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HBG1690112.1"
                     /translation="MNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAINYMK
                     KYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLVEDCEFIISNLNYLSNIIENSLN
                     KKTKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYTYEG
                     NDFPEYVPIIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNMLVY
                     VAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQILTMGSPKF
                     DSLIYNLKQNNINKDYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIENSQE
                     YGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSHALI
                     TDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYIVGEEDMDLIKFMNLVKN
                     NNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
     gene            complement(1833..2573)
                     /locus_tag="KPH93_003341"
     CDS             complement(1833..2573)
                     /locus_tag="KPH93_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1690113.1"
                     /translation="MGNATRIYEKRVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQVCDFINTLYDTKVKSKMPFDTIILAGVTMYINDDELKIALKN
                     LLGILDKKATIYISIPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLEEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            complement(2586..3230)
                     /locus_tag="KPH93_003342"
     CDS             complement(2586..3230)
                     /locus_tag="KPH93_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBG1690114.1"
                     /translation="MPTTKTIVINCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINDGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            complement(3203..3820)
                     /locus_tag="KPH93_003343"
     CDS             complement(3203..3820)
                     /locus_tag="KPH93_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HBG1690115.1"
                     /translation="MKGNEMENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNE
                     TMLGNLSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNL
                     DIWICDLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSND
                     IKMIENADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
BASE COUNT         1509 a          639 c          421 g         1776 t
ORIGIN      
        1 ttacaataat ttcattaaga ccatatattc taacattact ttccaattat atgcttagga
       61 gtacatatct atttaaagtt ttccctttta ttttcacttt tagggataaa aaatttgtaa
      121 ttttctctac ttttgataaa ttttatttta gactggttat ataggaatac agtctaaaat
      181 gttgtataga gtaacaaaat aaatttaaaa aaggtcaaac tttaattaat ttgcccttta
      241 ttatcttata tttattaaaa ttgataattt tttaactcct tcttaagcct aaatttatct
      301 aattatcaat taattttaac taaatatggt tattcaaaac gtattctaat acattagtat
      361 gtattttttc accacaactt ccatctgtat ttgatagtac actatttaaa gaatttaatc
      421 tttgatttag cttataatca ttattatttt ttactaagtt cataaatttt attaaatcca
      481 tatcttcttc tcctactata taatttgaaa atatatcgaa tattcttgaa tcataatatc
      541 cacgttccat atctatatca taaattaata taggtttctt cgtttgaata tattctatta
      601 atacagaaga cctatccgtt attaaagcat gggataagtt gaatgattca tagtaagata
      661 cagagtcatc taaaataata ttatttttta tagatattga atctagcaaa gttttgtatc
      721 tattaaaaca ttctggaact ttagatttta catactcaag ttctaatgga tgtggtctat
      781 atataagacc atactcttga ctattttcaa taatatcaaa tacattttca atctcatcaa
      841 tgacaccatc ttttccatta ggtattttca ttagattagt tgtccataag aaaattattt
      901 taccctttaa tttagattca taatctttat ttatattatt ctgttttaaa ttgtaaatta
      961 aagaatcaaa ttttggagat cccattgtta gtatttgatt tttatttata ccactacatt
     1021 ctgctataat ttcaaatgca ggagattgca caataacctt atcaaaattt ctagatgcta
     1081 tcagttcata aaaatttaag taaaaactaa caggataagt tccaagtatt ccataagcaa
     1141 catatacaag catattagta tattgtttca agttataaga aaaataatta ctatctacca
     1201 ttgtagcatt gttacaatca tcatattgat tatgaacata tattatatca ggcttctcct
     1261 tagataaatc aaaatcatca taatgtatta ttggaacata ttcaggaaaa tcattacctt
     1321 catatgtata tatttgagtt ataccattag gcgtatcaat taacttgtaa taaggtatgg
     1381 gaactacata gcagtcacac tgctcatcca aaaccgcaga tttccagata ctttctaagc
     1441 tattccacat tttagcatta taaggcataa atacaatttt agttttttta tttagactat
     1501 tttctattat atttgataaa tagtttaaat tacttatgat aaattcacaa tcttcaacaa
     1561 gtcttatatt tttatacatg gaaagtctta aaatttttac ataagtttca tcaattaaat
     1621 tcaacaggtt tttgttgctt cttccagagt tatttatata ttttttcata taattaatag
     1681 cttcaagaga tgaattaact attgattcac ttaaattatt attttttatg taatcaatac
     1741 cctctataag tatttgtata agatttaaaa cttcattttt tttattatag ttcatcagat
     1801 tcaccttaat tttgatttta tttatattaa aactatcgct ttagtataaa atagtgcctt
     1861 tctgtatcag agtattgatt tatatctgtt aagaagcttt tgctttcaac aatttcaaat
     1921 ccttcctcta ataatggttt taaaatttct tcatattctt ctatagttct atatataaca
     1981 ttatattctg aatttaattc tttcgagtaa aattctttta aagttaatct cttatttatt
     2041 ccaataggga tagatatata tattgttgct tttttatcta atattcctaa taaatttttc
     2101 aatgctattt ttaattcatc atcatttata tacattgtga cacctgccaa tataattgta
     2161 tcaaatggca ttttggattt aacttttgta tcgtatagag tattgataaa atcacatact
     2221 tgaaaatcac agtcattatc agaaaatctt tttttagcaa tagatattaa gtcttcagca
     2281 aaatctgtcc caagataata gttacatata ggaactatat catcagctaa cctacctacc
     2341 ccacaaccta tatccaatat atttgaattt ctagttatat ttagtttagg aattattatt
     2401 tctttttcat atttatctaa atttttagca taatcaggat tttggtcatt acatttcact
     2461 gaaatatagg gattctctgg aatatgcttt ttagctcgtt tattccaaaa atcttgaaca
     2521 gtatcttttt ttatatctac acgtttttca taaattctcg ttgcattacc catataaaat
     2581 ctcctttatt ttttgttcct ataccatttt aaagcttcat tatattcttc aggtgtatca
     2641 atttccctta attcaacatg cgctatttta agtggtaagt tgggtgaaat cattttatat
     2701 acatgatttg taccagagct taaattttta gaatatattt taaacaaacc tgtccactca
     2761 taatctccat ttgtccttga aaattctgta gcaaactctc catcattaat aaagtctgtt
     2821 ttaacataga cagggtcttt agtatagggt tgacatacac caataatttc tccatctgta
     2881 ttaacaaaat tatcgaaatc ttctttgaaa aaaagtatat caccatctag tgatataaca
     2941 gtatctctag aatatttttt acctaaagat aagcttgcag cagtcttagt gtttaagtaa
     3001 tcatgattaa atacaaacat tatgtcttta cgataagaac atacaacttc aattaatttt
     3061 tctgcttgaa agcctactac aatacgtatg tcatcataat catcaagaca ctctaattgt
     3121 cttattatta atggcttccc atctatatct attaacgact tatttgaatt taatcctagt
     3181 ctacttcctc tacctgcaca gtttataaca attgttttag tagttggcat aaacgttcct
     3241 cctcataaaa tgcataatca catactttta atattccagg agctataggt cttacccctc
     3301 caaatccaat tccaatatca gcattctcta tcatttttat atcattactg ccatcaccaa
     3361 ctgcaactat aggacctttc atttttttta taatatcttc tttttttatt ataactttaa
     3421 ttttatcaat gaaatcacca tttgaatgag ctatagatga ataatattta ttttctaaag
     3481 aaagctcttt cattaaatca caaatccaaa tatctaaatt atttgtcaca acagtacatc
     3541 tatctggatt ttcttttata aactttacaa ttttttcatt aagattaatc ttagcaacta
     3601 tacttttaac tttagaaata ggaatacatt ttaataaatc gactcttttc ttaaaacttt
     3661 cctcaaaaga taaatttccc agcatagttt cattagtaag attttgcata agttcatatt
     3721 tatttataca cttagaaatc tcaggaagta tttcttgttt agttaatgtt gaatctaaat
     3781 caaaaacaaa tttataattt tccatttcat tccctttcat tgaagcataa aatcctattt
     3841 ttgagtgata agtacatcaa acaatattaa gttagaaatt tcaataattt tacagagttt
     3901 ataattttat aacatatttt taatttttat tataaaacaa tatatggttc aatcaaaaaa
     3961 gctaaaaact aaatcttatc aaaatgaaga aatcttgcgt tcttaactaa caaaaatact
     4021 ctataaatgg tttttaagta ttaaaataac ttgataaaaa ttcaattgtt acaaatactg
     4081 ctgttttatg tcaagttaat tcaattattt aaaaacacta aaatcattga aattactaaa
     4141 aaattgtctt aatgtgcaaa atcttcgttt ttaggatatt gcccgattta attatataca
     4201 tattatcgtc taattttaaa aaaaattaag agaaaatatg tataattttc atttatattg
     4261 ttttttgttg tttatttttt gaacatgctt gtctatttta ttattataag caaatatgat
     4321 aaaattagtt tcccttgaat caaat
//
LOCUS       DAEMAE010000260         4308 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEMAE010000260 DAEMAE010000000
VERSION     DAEMAE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.264"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..1725
                     /gene="aspD"
                     /locus_tag="KPH93_003344"
     CDS             97..1725
                     /gene="aspD"
                     /locus_tag="KPH93_003344"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG1690116.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGISELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKNTSPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1846..2841
                     /locus_tag="KPH93_003345"
     CDS             1846..2841
                     /locus_tag="KPH93_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG1690117.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3026..3409)
                     /locus_tag="KPH93_003346"
     CDS             complement(3026..3409)
                     /locus_tag="KPH93_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690118.1"
                     /translation="MLNKKLPDIELKIMKYIWSTGYKTVISKDVADEIEKTYGWKHTT
                     TITLLVRLTKKDFLISQRIGKHVHYRVLIKEREYLKIEGKRIFGGLHNNPLSELILKL
                     HNEEEITEEKIIEIGSWIKSWKDED"
     regulatory      3637..3734
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3789..3965
                     /locus_tag="KPH93_003347"
     CDS             3789..3965
                     /locus_tag="KPH93_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690119.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENIVE
                     SCLHQVMVLISKYN"
     gene            4191..>4308
                     /locus_tag="KPH93_003348"
     CDS             4191..>4308
                     /locus_tag="KPH93_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1690120.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFK"
BASE COUNT         1537 a          545 c          673 g         1553 t
ORIGIN      
        1 ttctataaac ctattataat taaagtataa tcatttttgc aacttgttta ttttattata
       61 aaaatgtgaa acttattaca taaggaggaa tttaacatga aaaatcatac ttataaagaa
      121 atcaaaaaca tatatggaaa aataagccct tttgaattta aggacaaatt aatagatatt
      181 gcaaaataca ctgcaaaaga aaataacaga gaattacttg atgcaggtcg tggaaatcca
      241 aactggactt gttcaactgc tagagaagcc ttttttactt ttggtcattt cgccattact
      301 gaaactcgtt ctaattggga tttaggtcat ttggctggaa tgcctcaaaa aaagggtatt
      361 aaagaaagat tttttaaatt tataaatgaa aatatagata tgcctggagc atatttagct
      421 agagatatta taaactttgg tataagtgaa cttggatttg atggagatga gtttgttcat
      481 gaactagctg atggaattat tggtgataac tatccacttc cagatagaat gttacctcat
      541 atggaaaaaa tagtacacga ttacctagtt caagaaatga aatatgatat atcaggtaaa
      601 tatggtgatg tagaaatttt tgctgttgaa ggtgctacag ctgctatgtg ctatatattt
      661 gattcattaa tggcaaatga acttctaaaa aaaggagata ccatagcctt aatgactcca
      721 atatttacac cttatcttga aatacctaat cttcctcgtt acgactttaa agttgtaaat
      781 ataaatgcta atgaagttga tgaaaaaggt gctcacacat ggcaatatac aaaaaaagaa
      841 ttagagaaac ttcgtgacaa atctattaaa gctttattcg ttgttaatcc taataatcct
      901 gcttctatag ctatggatga aacatcttgt aataatctaa tagatgtaat tgaaaattat
      961 aataaagatt taatgataat atcagatgat gtatatggaa cttttgtaga agatttttca
     1021 tcattaatgt caaaactacc ttacaacact gttggagttt attcttattc aaaatatttt
     1081 ggagttacag gatggaggct tggtacattt gcccttcata agaaaaatgt ttttgataaa
     1141 aaaataaatg atttaactgg agaattaaaa aaatcagtag ataagcgtta cagtgatatg
     1201 agtcttaacc cttctagcct ttcatttatg gaaagagttg ttgctgatag cagacttgtt
     1261 gctctcaacc atactgctgg tctttctact ccacagcaag tacaaatggc gtttttctct
     1321 gcttttgcat tgattgacaa agtagatgcc tacaaaaaac tcaatatgag tatctgccat
     1381 agacgtcaaa aactattatt tgaagcttta gagcttccaa ttaatgaaaa caaaaacaat
     1441 gctgcatatt acactcaatt tgatattgaa gaatgggcta aattaaacta tggagaagac
     1501 ttatttaaat ttataagtaa aaatacatct ccagtagatg ttctttataa attagctaat
     1561 gactattcag tagttttact tagtggaaat ggattctatg gccctgaatg gtcaattagg
     1621 atatctctag caaatctata tgatgaagct tatactaaga taggaaaagc cataagagaa
     1681 atactaaatg gttatatcgt tgaatggcaa aaaattaaga aataattata ttacactgaa
     1741 atatctaaat tatatgaata aaagaattta tgtagacctt aactactttt tttgatataa
     1801 tcctatcgga attataaatt ttatttatga aaggcagaga atagtatgag tattaagaaa
     1861 aaagttgcca tagtttttac tggtggaact atatctatga cagtagatga taaagtcgga
     1921 gcagctatac caactttatc aggtgaacaa atactatcaa tggttaccaa cattgataaa
     1981 gttgcagatg ttgaagtttt taattttgat gaaattccag gacctcacat aacaccttat
     2041 agaatgatgg aattaaaaaa ttatgtaaat gatttattag caagaaaaga tattacaggc
     2101 gttgtaataa cacatggtac agatagttta gaggaaacag cttattttct agaccttaca
     2161 attgacaaca tcaaacctgt aatagtcaca ggtgctatga gaagtagttc tgagcttgga
     2221 tatgatggtt caagtaattt atctgcatca gtttgtactg cagtatccaa agatgctatg
     2281 ggaaaaggag tccttgtagt tttaaacaat gaagtattat tagcttctga agctactaaa
     2341 acaaatactc tttctctaaa tacatttaaa tctctaacta gtggcccatt gggaataatt
     2401 gactgcaatg aacttgtcct tacgagagat attgtaaata gaacaattat agatactgat
     2461 aaggtagaat ctaaagttgc tttatttaaa gcatatgtag gagaaaatgc tgactttata
     2521 agatttgctg ttgatagtgg ttataaaggt atagttattg aggcaatggg tagaggtaat
     2581 attcctcctc aaatgctttc tggagttgaa tatgctagag aaaaaggact tcctgtagtt
     2641 atagtttcta gatgtcattc aggtagagta ttcgatagtt atggttactt tggttctggt
     2701 agagatttaa aaaatcttgg ttgtatattt ggtggagact tgccaggtca aaaagctaga
     2761 ataaaactta tcttagcact tggaaaaact gataaccttg atgaaatcaa agacttcttt
     2821 gaaaaaggaa tctattgtta attctaatag cttctattta attatttatt gatatttcaa
     2881 catttttaaa tcaaaatcta atacttatgt cagttaaatg tcagtaaaac attttatcca
     2941 caatttttat ataagttttc cacaacttat ccccaatatt atccacaaaa aggtagttac
     3001 taatataagt gctacctttt atttattaat cttcatcttt ccagcttttt atccaacttc
     3061 ctatttctat tattttttct tcagttattt cttcttcatt atgtaatttt aaaattaatt
     3121 ctgataaagg attgttatgt aaacctccaa aaattctttt tccctctatt ttgagatatt
     3181 ctctctcttt tattaatact ctatagtgta catgcttccc aattctctgt gatattaaaa
     3241 aatctttttt agttagcctt actaaaagtg taattgtagt tgtatgcttc cagccatatg
     3301 ttttttctat ttcatctgca acatcttttg atattacagt tttataacca gtactccata
     3361 tgtatttcat aatttttaat tctatatctg gtaatttttt atttagcata atatattcct
     3421 tctttcaatt tatgtcactt cttcaaattt atctagctta aattaataat aggaactttt
     3481 tagaacaaaa actataggtg atttttacca agtattctat atgtttagtc tgttttttaa
     3541 gtgtaaaatt atgttcatat ctgtaaattt attcaatttt caaacaatct tgttgaataa
     3601 taactaattc tattgtatta ttttagttgt agaataagac taaatcggca aaactagaga
     3661 aatttagtga cgcaaagcta tagggactaa gacttatata aatatcttat gagttatgtc
     3721 agccagttgc caaagagata ttgttctttt tgtttttatg aaagtttttt agggggataa
     3781 taatttatat gtttggaaat aaaatggata aatgtacaca tgtattgact gcttatatta
     3841 gcagttcata tgattattgt aattttttag atacacagtt agatgatttt atattagagt
     3901 atggagaaaa tatagtagag tcttgtttac accaagtaat ggtattgata agtaagtata
     3961 attagataga cattgatttc tagagaaccc tttttagttt tatctacatt tatattaata
     4021 tatagcaatg taaaacgctt aaatgtattt ttaatattaa gaataactaa aatgttaact
     4081 aaaaaatata attaagtaga catttctatt aatctatgat ataatagtat ttgtggtaat
     4141 acacaattaa atcaagtata tcttgatata ggttaatagg agtattttta atgaataatg
     4201 gaatagtgaa atggtttaat aatgaaaaag gattcggttt tatatcaatg gaaggtagag
     4261 atgatgtatt tgctcatttc tcagctatac aaacttcagg atttaaat
//
LOCUS       DAEMAE010000261         4249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEMAE010000261 DAEMAE010000000
VERSION     DAEMAE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.265"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            285..950
                     /locus_tag="KPH93_003349"
     CDS             285..950
                     /locus_tag="KPH93_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG1690121.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKANIIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            972..1904
                     /locus_tag="KPH93_003350"
     CDS             972..1904
                     /locus_tag="KPH93_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG1690122.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1925..2566
                     /gene="pcp"
                     /locus_tag="KPH93_003351"
     CDS             1925..2566
                     /gene="pcp"
                     /locus_tag="KPH93_003351"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG1690123.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2686..3018)
                     /locus_tag="KPH93_003352"
     CDS             complement(2686..3018)
                     /locus_tag="KPH93_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690124.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3284..3904
                     /locus_tag="KPH93_003353"
     CDS             3284..3904
                     /locus_tag="KPH93_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1690125.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         1596 a          565 c          637 g         1451 t
ORIGIN      
        1 ctttttataa tataatatct aatttaaaaa tactatttca aacattgaca tatacttaaa
       61 ctatatactt acaaaatcaa ttcatattag cttaaaatat aatctttctt attcaaattt
      121 ctatacacaa ccattattta ctttgatact ttaaaacatc ttccctattt tttcttaaat
      181 tttataataa tagtcctatt aacgcaaata ctataaaaag agtttaactt tatcctttaa
      241 ataaatttaa attaaaacat atagaaatgg aaggagaaca atttatgata aaactaatcg
      301 gtatattgat agttgtaatt ggatttatac ttaaaattga cacattattt actgtattat
      361 tagcaggagt ggcaacagga attgttgcag gattagattt taatcaaatt tttacaattt
      421 taggagattc ttttgtctca aacagaggtg tatcattatt catattaaca ttgccagtaa
      481 tcggtgtatt agaaagatat ggtttaaaac aaagagctgt ttctttaata gaaaaattaa
      541 aaagacttac aacaggaaaa gtacttacta tatacatgat agctagacaa atagctggtg
      601 ctctatcaat aagaatgagt ggacatcctc aatttgtaag accacttgtt aatcctatgg
      661 cacaagctgc tggtttaagt aatagtgatg aactaaaaga atcggatgaa gaagccataa
      721 aagcattgtc tgctgcatca gaaaactatg gaaactttta tggtcaaaac ctatttgcag
      781 gtagtagtgg agtattactt atagcatcta ctctgactca atttggatac aatgtaactg
      841 gacttaatat tgtaaaggct aatattatta tggcagtaat agcacttgta gtagctacaa
      901 tccagtttac cttatatgat aaaaaattaa acaaaagtca taaaaaatag ttttatttag
      961 gaggaataaa aatgcaaaat tttataaaca tatcattgga aatcttttac gcactaatgg
     1021 gatttttaat gattgttata gcatataagt cttttactac cataaataac aataagaaat
     1081 atggtacatc attattttgg atacttatat cactgccatt catatttggt agattgattc
     1141 cagccaatat tataggaata atattaatat tatctagttt attaacactt tctaaacaag
     1201 ttgtatttgc aaagtatgaa gaaccagatg aaaattttgg aaaagaacaa gcagataaac
     1261 ttaaaaataa aatatttata ccatcgttaa tattagcatt tgctgcggta gtagtagcta
     1321 tgagcctttc taattttgat aattcttctc aatttgcgat aggtgtgggt tcaataattg
     1381 cattaatatc agcccttata ataaccaagg caaaacctgc tacatcagtt caagatggct
     1441 caagattact tcaacaaatg ggacctgcta gtatgttacc acaactttta gttgcattag
     1501 gagctttatt tactcaggct ggggtcggtg aagttatttc aaccatgata tctggtgtag
     1561 tccctgctga tagcagatta tttggtgtta tagcttatgt attaggtatg gttatattta
     1621 ccatgataat gggaaatgca tttgccgcat tttcagtcat aacggcagga attggtattc
     1681 cttttgtatt gtctcaaggt ggaaatcctg ctataattgg agcattagca ttaacagcag
     1741 gatattgcgg aactttgctt actccaatgg ctgctaattt taatatagtt ccagcagcct
     1801 tgctagaatg taaaaatgat tatattgtta taaaatatca agctccagtt gctttggtat
     1861 taattatagc ccacatatta gtaatgtact ttttaggatt ttaatataat taggaggtaa
     1921 aataatgaaa attttgttaa caggatttga cccatttggt ggagaaccaa taaacccagc
     1981 tcaagaagca gttgaaaggg tcaataataa tataaacggt gcagaaatca taaagataac
     2041 tataccaaca gttatgacaa aatctgttga agctatagat aaagctattc aagagcacaa
     2101 tccagatata gtaatatcag ttggacaagc tggaggtaga tttgacatta cacctgagag
     2161 agttgctata aatatagatg attttagaat aaaagataat gaaggtaacc aggttataga
     2221 tactattata aaagaagatg gtgaacctgc atatttttca aaattgcctg taaaagctat
     2281 ggtaaaacat atgaatgaaa ataaaattcc agctagtgtt tcaaatactg caggaacatt
     2341 tgtttgtaat catgtgatgt atggaatcct ttatatgata gacaagaaat atcctaatat
     2401 aaggggtgga tttatacaca tcccatatac aacttcacag gtaatagata aaaaaaatac
     2461 accattcatg tctttagaag aaatagtaaa aggtttagaa ttagctatag aagcttgtat
     2521 tatatacaaa gaagatgtaa aagaaattgg tggtgaaatt tcataataaa ttttttagta
     2581 tttttataaa ttaaaaatac taactattaa tttaaatata gactgtgagt agattattca
     2641 aacaatttta ctcacagtct ttttatggta tttacagata taaaattaag gtctattcct
     2701 ctctccccac tcacacattg catctaaaat tggaatcaga gattttcccc tttctgataa
     2761 actatattct accttaggag gtatttgagg atattcagta cgaataatca agttatctgc
     2821 ttctaattct tttaatgata aactcaaagt tttataggag atagtattta tataacgttt
     2881 taatgcatta tatcttacaa cttcaaattc tacaagacag taaagaatta tcattttata
     2941 tttaccacta atcagtgaaa gtgtataact aaatcctgtt tcacttaaat ctgtatttga
     3001 tatacagtct atactcatat attacactct ccttttggtt agtattgaac aaaaatgtgc
     3061 gtacttgttt ttagaataat ttataataaa attatagtgt aagaataatt gaaagtcaat
     3121 tttataatag gaggtgtatt tactaaaaaa agagttattt taaataggat tctaagatta
     3181 gacatttcta tataaattga taatattcat gaaaatccta ttttataaaa aataatatct
     3241 cttagaaaat ctattaaata aaattcactg gaggaaattt ataatgaaag ttttattgat
     3301 aaacggtagc ccaaaccaat atggatgtac ttatacagca ctaaatgaga ttactaaagt
     3361 attgagtaaa catgatatta aaacagaaat actgtattta ggtaaagaag caatcccagg
     3421 atgtattagt tgcgcatcct gttttgaaac tggaaaatgt attcgaaatg ataaagttaa
     3481 tgaattgatt gaagatctgg ataatattga tggtattatt attggttctc ctgtttactt
     3541 ttctagtgca actggtcaat taacttcttt tcttgaccgt ctatttttca tagctggaag
     3601 tcgcatggct acaaaattag gtgcttcagt tgtgtcatgt agacgtggtg gtgcaagtgc
     3661 aacatttgac caactaaata aatatttttc aatcagcaat atgcctattg tatcatcaca
     3721 gtattggaac caggtacatg gctttactcc agaggatgtt atgaaggacg aagaaggatt
     3781 acagactatg cgaactctag gagaaaacat ggcatggctt cttaagtgta tatcagcagg
     3841 taaaaaagct ggaattaaag agccacaata tgaagaaaca attatgacta attttataaa
     3901 ataaataaaa aatgcactaa tattaattaa aacaaaacac taatttataa ataaaataag
     3961 tataaatgta ggataaaaag cactaaaaca gaggtattga attttatatc aaacctctgt
     4021 tttattatta cattaataac caaaccatat ataacatcat atatttctaa atagattata
     4081 atataatatt ctaatgttat tgaaattaca ataactgaat ttctgctcat aattttaaat
     4141 attctacaaa tctcatctaa ataacaattt taattgacct aaaacaatcc tcatattgtt
     4201 cattttgtat taaaaatatc acttacaatc aaaaaccatt tattaagta
//
LOCUS       DAEMAE010000262         4239 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEMAE010000262 DAEMAE010000000
VERSION     DAEMAE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.266"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(141..734)
                     /locus_tag="KPH93_003354"
     CDS             complement(141..734)
                     /locus_tag="KPH93_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG1690126.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(805..1668)
                     /locus_tag="KPH93_003355"
     CDS             complement(805..1668)
                     /locus_tag="KPH93_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG1690127.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDEFTYKSLVNALEFKDDLYLENPKEENIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFRDCIEENDLIELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            1831..3000
                     /locus_tag="KPH93_003356"
     CDS             1831..3000
                     /locus_tag="KPH93_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1690128.1"
                     /translation="MLNIRTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVNPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNILSF"
     gene            3101..3577
                     /locus_tag="KPH93_003357"
     CDS             3101..3577
                     /locus_tag="KPH93_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG1690129.1"
                     /translation="MKEILINPIGKIRIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTTVQILNIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(3982..>4239)
                     /locus_tag="KPH93_003358"
     CDS             complement(3982..>4239)
                     /locus_tag="KPH93_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1690130.1"
                     /translation="DVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEE
                     VKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         1432 a          684 c          509 g         1614 t
ORIGIN      
        1 tctattatga ctaatcttca caatataaaa aatcgcagtt tagatattcc ttatacaaat
       61 ttataaggaa taataaattg cgatttttat ttttaagtaa tacgattaat cgatactact
      121 tcagtattta aattcatttc ttaatagtca tacttattcg tagtttcgca cattactacc
      181 tttatttttt tattaaacat attcttatat tctaataaaa actcatttag ttctgaatcc
      241 ataagattta tttttctatc actacaaagc attatctttt cttggtctat tattacatta
      301 atatcattta agtcattttt tataaatgtt gttatacaac tctttggttg gattttttcg
      361 ccatatttct cataaaatcc tgcactcttc ttaaatgact ctaaatcttc tgtagatact
      421 ttgctggaag tattattgct cataaaacct gctgtagaag ggtcgattcc aaagtttcta
      481 agttgttttt ctacttcttc agtgtccata ccatatctct ccataaattg cttttgtatt
      541 ttcataaatt tttcttgaga tatgttgtta tctctcatat aatcgaatat ttttcttgaa
      601 aactcagcca tagacatctt tccatccata acagaaaaat ataagttata tatgtattgc
      661 tcaaatttat ctacatcaga atctttaact ttgttcttta gttcgttaaa gtctattatt
      721 ctatcatcag acatttttca tcctcctagc taaattttta cttgtataag attataccat
      781 atttctatta tatttatata cacactattt ttttaattta acgatttttt ctgtagcatc
      841 taattcgcac attctgccca aaggaatagt tatcattggg atacaatgac ctgactgaag
      901 attatatata gaaggtttat tgaccttatt tgcaaattct attagtaatt ctattaaatc
      961 attttcttca atacaatccc taaaatctcc aaaaattata ccattacatt cctcaagttt
     1021 tcctgaagaa tatagttgtg ttaacattct atccagttta tattgaggtt ctccaatttc
     1081 ctcaataaac aaaattttat cttttgcatc tatttcatat tttgttccca ttgtggcaat
     1141 tattaaggat aaatttcctc ccattattat tccctctgct ttaccttcac atacagtata
     1201 aatattttct tcttttggat tttctaaata taagtcatct ttaaattcta aagcatttac
     1261 caatgattta taggtaaatt catcccattt acctacattc gctgccatta ttccatgata
     1321 tggtaaaagg ccacaatttt tataaaaagc taaatttaaa cctgttatat cagaaaaacc
     1381 tataaatggt tttggatttt gttttattat atcataattt attttatcca atattcttgt
     1441 tgttccataa cctcctctta agcacataat tatatctaca tctttatcta aaaacatatc
     1501 ttctatatct cttgctctaa tatcatcatt accagctaaa taacctttat aatttaaact
     1561 acaactttca gaaaacttaa cttcgtaacc ataactttct aaattaaact ttatttcctc
     1621 caaacttgta tttcttaatg gcccagatgg cgcaattatt cctaacattt aaacacctct
     1681 tcttataaaa tgatatattc ttctccatac taaatatctc taatataatt ataagatatt
     1741 ttatttgtac ttgatacata ataatgattt acataaaata atataattaa tgaagaaaag
     1801 taaattttta atatagttag gatgtgtttt atgcttaata taagaactct ttgtcatgag
     1861 ataaatgatt gggttataaa tataagaaga gatttacata agactccaga acttggatta
     1921 gaagaatttc aaactaagaa aaaaattatt aaatacttaa atgaaatagg tataaattat
     1981 attgaatata agaatcatac agggataact gcatacatta acgtgaatcc aaactttgaa
     2041 acagttgcta taagagctga tatagatgcc ttacctataa cagaagaact taattattct
     2101 tataaatcta ttaatattgg taaaatgcat gcatgtggcc atgatgcaca tactgctatt
     2161 ttactgggta cttgtaatat actatttaaa ttaaaggatt acttaaatgt aaacgtaaaa
     2221 ttcttttttc aacctgccga ggaaactatt ggtggggctc aacttatgat agaagatgga
     2281 tgtcttgaaa acccaaatgt aaaatatata tttggacttc atgtaaatcc caatatcaac
     2341 aaaaatctaa ttgaattaaa atacaacact ctaaatgctt ccactgatac acttcaatta
     2401 actgtacatg gttcaaaatg tcatggagct tatccacatc aaggtattga tgctattgtt
     2461 atatctgctc atattattac agctctacag acaatcgtta gtcgaaacac aaatccaact
     2521 gattctgtgg ttatttctct aggaaagatt gaaggtggaa tcaaggaaaa tatagtttgt
     2581 gataaagtag ttataagggg tactttaaga accttgactc ctgaaactag agagttttca
     2641 aaaaaacgaa ttagagaaat ttgcgatttt acttgtaaaa catttggagg aagtatttct
     2701 gttgaaattg aggaaggtta tccagctctt atcaattcaa accatctagt agattacgtc
     2761 aaacaaaatg ctgtagaatt gtttggagaa gaaaatatta tccttaaaga ctcgcccact
     2821 cttggggctg aagacttttc gtattttcta agacactgtg aaggagcttt ttaccacctt
     2881 ggttgtgcaa atcgtgaaaa aaacattact tcccctttgc atacctctac ttttgatatt
     2941 gacgaagatt gccttattac tggtgtcatg ctccatgtaa aaaatatatt atcattctaa
     3001 ctattaatat ttttatttat tattttatat tgaccctcac atgatgtcat gttataccct
     3061 aaaattacaa atgataaatt aaatagttgg aggtaatatt atgaaagaaa tattaataaa
     3121 tcctataggt aaaatacgta ttcaaggcga agaggtcttt attaaattag acaaaaagta
     3181 tgtacctgca ttaaatgaac tcgacgattt cagtcattta aatgtatttt ggtgggctga
     3241 tgggtttgat aatcctgaac taagaagtat tttagaaacc ccaaaacctt ataaaagtgg
     3301 accagatgtt ataggaatat ttgcaacaag gtcacctata agacccaacc caatagcatt
     3361 gactactgta caaattctca atatagacca tgaaaatggt ataataaaaa taccatatat
     3421 tgatgctaat gataatagtc ctgttattga cttaaagcca tatactccaa gcgttgatag
     3481 ggttgaaaat ccgtcagttc ctaaatggtg tagtcactgg cctaagagtg tagaaaaatc
     3541 aggagaattt aattgggaag atgaaattga attttaaatt acttttttct tgatttcatt
     3601 aactcgtttt ccacatttat tttatttaca cttaccaata ttttatgtca catatgcata
     3661 taatattagg ctgtattata taatacagcc taatattata taataaattt ctatttttcc
     3721 cttaaaccat ccacattctt tctaagctac ataagatttt tctattattt ctttttgagt
     3781 ttaatttagc attatgttga ggaaaaaaca tttcatgcat ttttaataaa ttaaattttt
     3841 cttttattct acgattttat aatactgaac ctcccatgac taaagtcaca gggttcctgc
     3901 ttcatagaat tttgcatact aaaatatgtc ttactaattc tccacaggct atccccgtaa
     3961 ttcctacggt tcttacataa gttatttatt ttgaattgtt attagtctta atccttcttt
     4021 tagtatattt atacttgcat ttatatctct atcgtgagtt tcattacaac taggacaaat
     4081 ccattctctt atatttaagt ccttaacttc ctcgttttta tacccacatt tattgcatat
     4141 ttgtgaactt gcaaagaatt tacctacttt tacaattttt ctaccatacc aattagcctt
     4201 gtattctaat tgacgaataa attctgacca tgatacatc
//
LOCUS       DAEMAE010000263         4180 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEMAE010000263 DAEMAE010000000
VERSION     DAEMAE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.267"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1059..1448
                     /locus_tag="KPH93_003359"
     CDS             1059..1448
                     /locus_tag="KPH93_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690131.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2264..2734
                     /locus_tag="KPH93_003360"
     CDS             2264..2734
                     /locus_tag="KPH93_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG1690132.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSDGYSVEETSKIIQHPIEFVKQCENSKE"
     gene            3054..3923
                     /locus_tag="KPH93_003361"
     CDS             3054..3923
                     /locus_tag="KPH93_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG1690133.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDIKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT         1815 a          307 c          672 g         1386 t
ORIGIN      
        1 aaaaaatata atgacatcct aaaaaagttt taattatacg ttgttctgtg tagttataat
       61 agcttctatg taactattgt aagtatattt tattatagtt atttatttaa agtacgataa
      121 atgttttaaa ttgaagtatt ttgcccccct tgagttctac taaagtagag tttttgtgta
      181 gtatttttag aaataacata tatatatatg tatttatgtt gaaaaaaata gaaaaattat
      241 aatataatta ataaaaaaat ttaaaatttg taatatataa ataaaaaaac tctaaaatag
      301 aaggatataa ttatatagaa tgtttcaatt aaacaaggca aaaactataa tcttaaaatt
      361 aaaattaaca ttattcttaa gaagtaagaa atttaagaag taaaaagttt agtagtaagt
      421 tatagcaagt aagaaattta gaaagtagga agtttagtaa taagtcatag tgagtaagaa
      481 atttagaaag taggaagttt agtgataagt ggtagtaagt aagaaattta gaaagtggaa
      541 agtttagtaa taagtcatag tgagtaagaa atttagaaag tggaaagttt agtgataagt
      601 agtagtaagt aagaaattta gaaagtagga agtttagtaa taatttgtag caagtaagaa
      661 atttagaaag taggaagtta gtaataagtt gtagtaaata aaaaatttag aaagtaggaa
      721 gtttagtaat aagttgtagt aaataagaaa tttaggaagt gggatttact gaaatacaaa
      781 tatttaaaat caaagagttt aaaagaaagg agagctactg gacaagtatc caaaatagat
      841 agctgagcac aaaatatttc ataaaattaa gcaatataat acgagagaat tttgacatta
      901 tttatatata tttaacgtgt acaaatagct aaatttagga ttaagattga aaaatatctt
      961 ataagtctga gcttatataa atccgtataa gatgtattta atcgaaaaat tttattatgg
     1021 atattgcttg tctatattct gagtagcaag gttttatcat gactataaaa aagttgccac
     1081 aatcagaatt aaagataatg aaatttatat ggaaatcaga ttccaaagta acatctagag
     1141 atattgtttt aggaatggag caaaaatatc aatggaaaca aacgacaact ttgactcttt
     1201 tatctagatt agtagtaaaa agatttctaa attctcaaaa gatagataaa tatacacatt
     1261 atgaagtttt aattaaagaa aaggaataca taggagttga gacaagagat ttttttagaa
     1321 atattcatga ttcttctata aaaagtttat tgttgtcatt acatgaaaat attaatttga
     1381 gcaaagatga tatcttactt attgaagagt ggataaaaaa tctaaaagaa gaggagaaag
     1441 acgtataagc aaacgtattt cttatagcat caatataaag tgtatgtaag tgtatttatg
     1501 taatatatta gtctacttaa gtaatatgac aaatgattat aagtggatta tgatactata
     1561 atgtgaatct aaataaatta agtataaatt gatagtggat attctattta attaaattta
     1621 aaaagctacc tttacttata agtataatta aggtagcttt ttaaatagag gtagtgtgat
     1681 taaagagaat ttttaaatat aaactgtaga gaattaaagt aactttttaa atataaattg
     1741 tgaagcttta atatagatga caatatatat tttgcaaata tgacacagtg ttttcgtatt
     1801 taagatgata aaataaatag gaattaatga agatattgaa aaaataaaaa taaaagatta
     1861 atagaagata ttgaaaaagt aaaaacaaaa gattaataaa agatagtgaa gagatagaaa
     1921 caaaagtgtt aatagaagat attgaaaaaa taaaaacaaa agattaataa aaggtactgg
     1981 agagatagaa acaaaagtgt taatagaaga tattgaaaaa ataaaaacaa aagattaata
     2041 aaaggtactg gagagataga aacaaaagtg ttaatagaag atattgaaaa aataaaaaca
     2101 aaagattaat aaaaggtacc gaagagatag aaacaaaagt gttaatagaa gatactaaaa
     2161 aagtaaaaat aaaggattaa taaaagatac tgaagagata gagacaaaaa tgttaataga
     2221 agatactgaa aaaaatagaa ataaaaaatt aggaggaaaa tttatgaaat atcaggtttc
     2281 attattagca gtgaaggata ttgaagtatc aaaaaaattt tacaaagaat tatttgaaca
     2341 agaaattgaa ttggacttag gtaaaaatgt aacatttaaa ggaggatttg ctatacaaga
     2401 agacttcgca tggcttacag ggattaatcc agatacaatt atacaaaaat caaataatat
     2461 ggaactttat tttgaggttg atgattttga cgaatttatt gaaaaactca attattataa
     2521 agatgtagag tttgtccata agccactaaa acatgaatgg caacaaagag tagtgcgtat
     2581 ttatgaccca gataagcata ttattgaaat aggagagtct atggaagtta tagctagaag
     2641 gtatttatct gatggatact ctgtagaaga aacttctaaa ataattcaac atccaattga
     2701 atttgtaaag cagtgtgaaa atagtaagga atgattgttt aaattataat tgtttagtag
     2761 aaattaattg tagtggaact aattcaagat aatatatggg actagtattt gacaaatgtt
     2821 ataaattaaa ttaaaataaa gaaaatatta gtaaagaaaa aataaagaaa aatttaataa
     2881 agaaagataa ggggaatatt aataaagaaa aaataaatac aatattaaaa tatattaata
     2941 aatttgaata tttataatgt tttgaatttg aagtggaaat acttaaacgt ataaagctta
     3001 ttatcatgaa atttctatat taatatgtag taattcaata ttattataaa ggagtggtta
     3061 ttattaaggc aattgaactt tataaaaaat tagatttaga atttaatatt aaaaatatta
     3121 atgatgattg gagttttatg aattttgaaa atgattttat cactccagaa tttagaaaaa
     3181 agtacatagg attagtatta gataatgctc aaaatattaa taaagtatac acgactactt
     3241 ttcctgataa agagataatt aaacaggtta tagataaaga tgaaaaagat attttaattt
     3301 tttcacatca tgcaatggga tacatagcat cagatgaagg ttttccattt cacgacatac
     3361 cactatctta tatggaagag atgaaaaatc gtagaatttc tttttatgta ttacattctc
     3421 cactagacaa ctatagtgac tattcaactt ctgttagttt tgcaaaagcc ctaggtttag
     3481 aaatagtcaa accattttgc aaatatgacg ataaaattga ggtaggagtt atatgtaaga
     3541 catcactaaa atcaatagaa gagattaagg aattaattaa aaaatctgtt ggtcatgata
     3601 ttaaattgta tgattatgga gattctgtat taaaagatgg tttagttgct attgcagcag
     3661 ggggaggaag ttatccattt gtagcaagag aagttgctga acttggcata aatctatata
     3721 ttactggatt tactaaacct ttaaaacatt ttgaaccagt attagagttc catcaaatag
     3781 caaaagataa tttaattaat gttataggtg ctactcatta ttctacagaa aaatttgcat
     3841 gtatgggcat ggtcgattat tttaaaaatc taggtttaga atcagaattt ttgcaaggaa
     3901 agtattactt agaggattta taggatatat taaacaataa aaataagcag ttagtaaaaa
     3961 tttgttttac taactgttta tttttttacc catttatcca gtgttttgaa aaaatctatc
     4021 tttatttttt aaactaagaa aatagacttt taattgctga ttagagaagc tttagttttg
     4081 atgtctttta aattattttt cgatattttt tagtttattt tttgatgtat tttagattat
     4141 tatttaggat tgattagaat ttataaatat ttattttaga
//
LOCUS       DAEMAE010000264         4088 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEMAE010000264 DAEMAE010000000
VERSION     DAEMAE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.268"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..2337
                     /locus_tag="KPH93_003362"
     CDS             67..2337
                     /locus_tag="KPH93_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1690134.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRQKGGPCWSAGMHAQKYDFGIARSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLYSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALSAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISEFIEESLKNTSNKVNYASNELDTTSNKVNYVLDELD
                     ITSNKVNYVSDELDITSNKVNHVSNELGMASNKINYVSDELDITSNKTDYISNELYNL
                     SNKSIKDNIPIKMVVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFMTDTATLCDLF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPVHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDENLDIEKLKNNYFTIHSPVAWEDKKFETPSGKYELYSERIKS
                     LGISPTPVYISNRYREIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQSMAKK
                     VGIKDKDIVTLRSKKAKINVQINIDDGVGNHIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            2424..2759
                     /gene="spoIIAA"
                     /locus_tag="KPH93_003363"
     CDS             2424..2759
                     /gene="spoIIAA"
                     /locus_tag="KPH93_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG1690135.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2773..3201
                     /locus_tag="KPH93_003364"
     CDS             2773..3201
                     /locus_tag="KPH93_003364"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG1690136.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3224..3988
                     /gene="sigF"
                     /locus_tag="KPH93_003365"
     CDS             3224..3988
                     /gene="sigF"
                     /locus_tag="KPH93_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG1690137.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         1678 a          395 c          776 g         1239 t
ORIGIN      
        1 gtttaataaa ttatagtgta tatgttaatt tttaataagg taagaaagta gagagaagga
       61 tattttatgg atataataaa aaaattaagt catgggtgta cattagattg tcatgattgt
      121 tgtaaattca atgtttatac taaaggaaat aatgtagtaa aaattgaagg agataaaaat
      181 catccttaca caaaaggttt tatatgcaaa aaagggatgg ctcatttaga tagattaaat
      241 cataaagata gaataaaaac tcctatgctt aaagttgatg gagtatggaa agagatttct
      301 tttgataagg ctatagaaat aatggcagaa aagcttacat actataaaga aaagtataca
      361 tcaaagtctg tcatgcatta tgaccaatat ggaagtgggt ctgtgctaaa atacatagga
      421 gatatatttt ttaattttta tggaggtgtt agcagacaga aaggaggtcc ctgttggagt
      481 gcagggatgc atgctcaaaa atatgatttt ggaatagcta ggagtcatgc gatagaagac
      541 atgttaaata gtaaaagtat atttgtgtgg ggaaaaaatc cagcatatac tacaattcat
      601 actatgcaaa taattaaaaa agctaaagaa aaaggtataa agatagtagt gattgacccc
      661 atatatacaa aaacagctca aatcgcagat aagtatgtac aggtaaatcc tggaacagat
      721 ggagctttag ctatagctat ggcaaaaatt atagtagaag acaaattata tgatgaagaa
      781 tatataaact catatgttat tggttttgaa gaatataaaa aatatttata ttcgttggaa
      841 ttaagttttt taattgatga atgtggtgtt aaagagaatg atataagaga gttagtggat
      901 ttatatacta acaaatattc aagtataaat gttggatatg ggcttcaaaa atataaaaat
      961 ggtggaaaca caataagagc tatagatgct ttaagtgcta taactggtca aataggattt
     1021 agtggtggag gggtaaacta tgctaataaa gtttatccaa gtgttataaa ttctgaccct
     1081 tacaatagcc aatcatatgg tgaagatagg gagttttatg taagtaatat cagtgaattt
     1141 atagaagaat ctttaaaaaa tacatcaaat aaagtaaatt atgcatcaaa tgaacttgat
     1201 acaacatcaa ataaagtaaa ttacgtatta gatgaacttg atataacatc aaataaagta
     1261 aattatgtat cagatgaact tgatataaca tcaaataaag taaatcatgt atcaaatgaa
     1321 cttggtatgg cgtcaaataa aataaattat gtatcagatg aacttgatat aacatcaaat
     1381 aaaacagatt atatatcaaa tgaattgtat aatttatcta ataagtcaat caaagataat
     1441 attcctataa agatggttgt gataactaaa agtaatatgc tgaatcaact gccagattta
     1501 gtagaattgg agagggtatt ttcgaaaatt gaatttaaag tatgttttga tatgtttatg
     1561 actgatacag ctactctatg tgacctgttt ataccttgta caaatacact agaaagtgaa
     1621 gatataattt atagttcaat gaccaatcca tatataacct ataatgaaag agctgtaaag
     1681 ccagtacata aattgatgga tgaatattac ttctttatgg agctagctaa aaagatgggg
     1741 ctaaatgatt atccatttgt ggaaaagaga acttatttag aaaaagtgat tgaaccactt
     1801 aagcgttttg acgaaaatct ggatatagaa aaacttaaaa acaattactt tactatacat
     1861 agccctgttg cttgggaaga caagaaattt gagactccat ctggaaagta cgagctttat
     1921 tcagaacgta taaaaagttt aggaataagc ccaacaccag tttatataag caatagatat
     1981 agagaaatag aagataaaaa tatttctttt agattattaa ctaatcatca tgcagatact
     2041 ttatttagtc agcattttat ggataaaaaa tctatagctc aagcatatat aaatcaaagc
     2101 atggctaaaa aagtaggtat taaggataaa gatattgtga cattaaggtc aaaaaaagca
     2161 aaaataaatg tacagataaa tatagatgat ggagtaggta atcatatagt aaaaatgtat
     2221 gttggttggt ggaagaaaca tggaaatcct aatagtttaa cagacactgg tatatcagat
     2281 tttggtggtc aggtaacata taatgaaagt atggtagaaa taatacgaca aaactagtta
     2341 aatttcattg aaggaataaa aatataatta tagaattgat taaaagttac tatttttaca
     2401 aaactattga aggagggatt ttgatggtaa attattcctt agaacataag aatctataca
     2461 tagaatttat gtgctcagaa ctagaccacc atgtagccaa tgaaataagg gaagaaatag
     2521 ataacttatt aagtgtaaat caagtaaaaa atgttgtatt taattttgaa aatattaatt
     2581 ttatggattc ttcaggtata ggtgtcatca taggtagata taaaaagatt tctaatgaag
     2641 ggggacgagt ttcagtaatt aatataagtt cacgagttaa gaagattttt gatttatctg
     2701 ggttaaataa aattattggc atttatgata cgtatgaaga agctctaagt tctttgtagg
     2761 gaggagtatg tgatgaataa tattatggaa gttaaatttt cggcaatatc tgaaaatgaa
     2821 agtttagcaa gagttatagt agcatccttt gcagctaagt tagacccaac agtagatgag
     2881 ttagtagata taaaaactgc tgtctctgag gctgttacga atgctataat acatggatat
     2941 gaagaagaca gctctaagtt tgttttctta agatgtgaga tagaaggaaa tactataaag
     3001 attatagttg aagatgaagg gtatggaata gaaaatgtgg agaaggcaat ggaaccactt
     3061 tatacatcaa aaccagaact tgatagatca ggaatgggat tcacagtaat gaaaagcttt
     3121 atggacgatg tagaggtaag ttctgtaaaa gataatggga ctagaattga aatgactaaa
     3181 aaaataaatg tgcctaaata aagtccaaaa gtgaggtttt attatggaag taactgttgc
     3241 cagagaagaa aaaaaacctc ttcttagtca tgaagagact ctagaactta ttgaaaaagt
     3301 tcaaaatgga gatgaagagg caaaagaaat attaatatca agcaatttag gtctagttag
     3361 gagcgttgta tctagatttt taaatatagg atatgataga gaagatttat ttcagttagg
     3421 ttctataggc ttaataaaat caatatataa atttgaccca aaatttaatg taaaattctc
     3481 tacttatgca gtaccaatga ttttaggtga aataaagaga tacttaagag atgatggtat
     3541 gataaaagta tcaaggtcgt taaagcaaat agctgtaaag gcaaagatgg aaagtgaagc
     3601 tttaactaaa aaactaggta gagagccgag tatagaagaa ttagctaaag caatagatgt
     3661 agaaaaagaa gatttagtaa tggctatgga agctaatttc aatgttgagt atttgcaagg
     3721 agttattcat gaagaggaag gttctccaat atgtcttatt gataaaataa gcatgaaagg
     3781 agaaagtgaa gaagaaaaag tagtggataa catattgtta aaagacatac ttggaagatt
     3841 ggataagaga gagcgtcaaa taatagttct cagatacttt gaagatatga ctcaaagtga
     3901 aataggagag atgctcaata tatcacaagt tcaagtatct aggatagaga agaaagtatt
     3961 gtctaagtta aaagaatata tatcgtgata atacttgtga tatgtagaat aacaaaatat
     4021 aataatataa taaatatatt actaaaataa aatgtaataa ctttttatgt taagttttta
     4081 aattatta
//
LOCUS       DAEMAE010000265         4069 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEMAE010000265 DAEMAE010000000
VERSION     DAEMAE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.269"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..2249
                     /gene="nrdE"
                     /locus_tag="KPH93_003366"
     CDS             150..2249
                     /gene="nrdE"
                     /locus_tag="KPH93_003366"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG1690138.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     DLEERLKYLIDEGYYSKEVMNQYSIGFIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGTIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIEKNQT
                     FEGFDKSEYAKGRNSKVLSKYYEQSYLPKSEKVKALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFITDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2262..3233
                     /gene="nrdF"
                     /locus_tag="KPH93_003367"
     CDS             2262..3233
                     /gene="nrdF"
                     /locus_tag="KPH93_003367"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG1690139.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQVFWEQNVKQFWLPEEISVSKD
                     IKVWNELSDKEKELYKKVLGGLTLLDTKQGNNGIPSMMNLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KIDKEILEEKMYSILYSLMENEIEYTNVIYKESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3309..3935
                     /locus_tag="KPH93_003368"
     CDS             3309..3935
                     /locus_tag="KPH93_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1690140.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEVPKQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKCLDVLIKEDLIYT
                     LMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLRRKSYIHHEIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1610 a          445 c          769 g         1245 t
ORIGIN      
        1 atatgtagtg cttttgtttt tgaaaaatac ttattgcatt tctataatta tagattaatt
       61 ttgggatttc aactgataag taccaaaaga aataataagc aaattaaatg aaattatggc
      121 acaagattat gttaaaagga cggtaaataa tgaattggat agagcttaac aatcaagtga
      181 taattaaaaa tgaacaaggc aagtaccaat tggaaaagga taaggaagcc ttaagtagtt
      241 acatagaaga atttatacaa ccaaagttga gaaaatttaa tgacctagaa gagagattga
      301 agtatttaat agatgaaggt tattattcaa aagaagtaat gaatcagtat agcataggtt
      361 ttattaaaaa tatatatgat atgattgata aacataagtt tgaatttcaa tcatatatga
      421 gtgcaaataa gttttatcaa aattatgcat taaagagcaa tgatggaaag gaaatattag
      481 aaacatataa tgataaagtg ttaattgtag ctttgacact aggaaatgga gataaagatt
      541 tagctttaaa cttggcagat aaaatagtaa aacaagaatt tcaaccagct acacctactt
      601 ttttaaatgc tggaagaaaa agggctggag aaatggtatc atgtttttta ctatctgtag
      661 aagatagtac agaaggtata tcgtatgcta tatcttcatc aaatcactta tcaaagattg
      721 gaggaggagt tgcactaaat ttatctaaac ttagagcatc tggcgaatct atcaaagata
      781 tagaaggtgc agctggaggt gttgttggag tagctaaaat gttagaacaa tcatttagtt
      841 actttaatca aatgggagct agacaaggtt ctggtgcggt gtaccttact gtgttccatc
      901 ctgattttga attattgatg gatactaaaa agataaatgc agatgaaaaa ataaggcttg
      961 ctacattatc gttaggagct ataataccag ataaatttat ggagttggca gaaaaaaatg
     1021 aagtagcgta tgcattttat cctcatactg tgtataaaaa atatggagtt tctcttgatg
     1081 agatagagat ggatgagtgg tatgataaat tggttaatga tagtgatata agaaaaaaag
     1141 aaataaatcc taggcagatg ttgactaaga tagcacagat gcaacaagaa agtggttatc
     1201 catatgtagt gtacatagat acagctaata gagaacatac attaaaagat gttggaacga
     1261 ttaagatgtc taacttatgt tgtgagatat ttcaatatca aacaccttca gaaatagagg
     1321 ggtatggagg aaaaaatgaa tggggacaag atataagttg taacctaggt tctttaaata
     1381 tagccaatgt gatggataat aaaactatag aaagtacagt tgaaacagcg ataagagcac
     1441 tgtcatttgt agcagatagc acagatataa aaccagtgcc aacggtaagt aatagtaaca
     1501 gcaagtctca ttctatagga ttaggagcta tgaatttaca tggatactta gtaagagaaa
     1561 atattttata tacttcagat gatgctatag atttttctaa tgtatttttt gcaatggtta
     1621 gatactattc aattaaagct tctatgaaaa tagctataga aaaaaatcaa acttttgaag
     1681 gctttgataa atcagaatat gctaagggaa gaaatagtaa ggttttatca aaatactatg
     1741 aacagtctta tctacctaag tcggaaaagg taaaagcatt atttgaaggg atttatatac
     1801 caacaaaaga agactggaca aaattattgg atgaggtaaa agaaaaaggg atatataatg
     1861 catatttaat ggcagtagca ccaactcaaa gcattagtta tgtccaaaat gctacatcaa
     1921 gtataatgcc aattactgag ccagtagaag taagaacgta tggagattct actacaatat
     1981 atccaatgcc atttttaaca aatgataata tgttgtatta tcaatcagca tacaggatgg
     2041 atatgagaaa ggtcatagat ttagtggcaa cagttcaaaa ccatgttgac caaggtatat
     2101 caacaacatt gtttatcaca gatgaaaaga caactagaga tatagctaga cattacatat
     2161 atgcctataa aaaaggtttg aaaagtttat attacacaag aacgaagatg acaagagaca
     2221 ctcatgagtg tttagtgtgt tcagtatagg gagagaaata tatgacattt aatttacata
     2281 aaatacataa tgcagttaac tggaatagag aagaagatgg atttacacag gttttttggg
     2341 aacaaaatgt aaaacaattt tggcttcctg aagaaatatc tgtttctaaa gacataaagg
     2401 tatggaatga actaagtgat aaagaaaaag aattatataa aaaagtattg ggtggtttaa
     2461 cattattgga tacaaaacag ggaaataatg gaataccatc tatgatgaat ttaacagaaa
     2521 acctgcaaag aaaagctgtc ttatctttta tggggactat ggaagaaata catgcaaaaa
     2581 gttattcttc tatatttatg actttacttt ctaatttaga aatagatgaa ctatttgaat
     2641 ggatagaaac agaaccaaca ttacaaagaa aggctgattt agttttagct caatacgaaa
     2701 atactacaaa tcaagaggga ttatacctat caatggttac aagtgtattt ttagagagtt
     2761 tcttgtttta ttcaggattc ttttatccat tgtatctatc tggtcaagga aagatggtag
     2821 ctagtggaga aataatatcg cttattctta gagatgagtc tctacatggt aagtacatag
     2881 gattgcttgc acaagaaata tatgattcat ttgataaaat tgataaggag atattagaag
     2941 agaagatgta ttctatactt tatagtctta tggagaatga gatagagtat acaaatgtta
     3001 tatacaaaga aagtgggctt gaaaaagagg ttgtgaactt tctaaagtac aatgcgaata
     3061 gagctttaga aaatctagga tttgagaagc tatatactgt agatgctata aatccaattg
     3121 tattaaatgg tcttagcact gaaactaaaa cacatgattt cttttcaact aagggtaatg
     3181 gataccaaaa aggagtgtat gaggaattag aggatgaaga ttttataata taatttataa
     3241 ttatgacaca ctgttgtcac ttttagcgtg ataatatcaa tgtataaaat atatacggag
     3301 gtatttttat gaaatatgaa tggagaaaaa gagataaaga gctatattta cctaaaaaga
     3361 atcctgtaat aattgaagta ccaaaacaga aatttatcat gttaaaaggg agtggagacc
     3421 caaattctaa agaatttact gaagcaatag gagtacttta ttctttggca tatgcaataa
     3481 aaaccatgcc taaaaaaggc attgtaccag aagggtattt tgactatacg gtttttcctt
     3541 tagaaggtat atgggataag ggagaaaaaa gcaaatgttt agatgtactt attaaagaag
     3601 atttaattta tactctaatg atacgtcagc cagattttgt tacacaagac ctgttggata
     3661 aagctactga tatagtaaag aagaagaaac ctcataatct tcttgaaaaa gttagatttg
     3721 ggtctattaa tgaaggttta tgtgttcaaa tgcttcatat aggttcatat gataatgaac
     3781 cagaaagctt tgatattatg aacgaatttt gtaaaaaaaa taaacttaga agaaaatctt
     3841 acatacatca tgaaatttat ataagcgatg ctagaaaagt atcgcctgaa tcattgaaga
     3901 cggttttacg ttttgaagta gaaagggaag tataaagatt ttgaataatt tataagagtt
     3961 ataaaaagtg aagtctgttt agacttcatt ttttgatgca gttttaaaat taaatttgat
     4021 gtttcataac atttttaaaa ctttctactt ttattagtgc aaataactg
//
LOCUS       DAEMAE010000266         4054 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEMAE010000266 DAEMAE010000000
VERSION     DAEMAE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.270"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..2069
                     /locus_tag="KPH93_003369"
     CDS             57..2069
                     /locus_tag="KPH93_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1690141.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TADELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKGNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEHLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2311..>4054
                     /locus_tag="KPH93_003370"
     CDS             2311..>4054
                     /locus_tag="KPH93_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690142.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKAVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKTLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIGIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFNTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIVESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSDIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEME"
BASE COUNT         1748 a          434 c          665 g         1207 t
ORIGIN      
        1 caaaagttaa aagattaaaa ataaagtaga aatttatgtt aagtaaggag attaatatga
       61 gaagaaatac aaaattatta acaacaggga ttctttcaat ggcaattgtt gcacctacaa
      121 tggcatttgc tactgaatct aatgctatgg aaaataacgc tgatttaaat ataaacttag
      181 agaaaaaaag tatcgtttta ggtagcaaat caaaagttag tgtcaaattt aaagaaaaac
      241 cagatgcaga tagcattaca ttaaagtata aatgctatga catgccattg gatacaactc
      301 taaattacaa tcaatcaact ggggcatatg aaggaactat caattataac caagacccag
      361 aatatctaaa tgtttgggaa ctacaaggga taacaataaa cagcaaaaat aatcctaaaa
      421 ctttaaacag acaagaccta gaaaagatgg gattaaattt aaaagactat aatgtaacac
      481 aggaatgtat aattgaagac ataacttcta gaaaagatgt aaataaatat ttgagaaaaa
      541 cttcttcacc tattacagaa cttacaggaa gtgatagata cgaaacagca gttaaaataa
      601 gtaaagaggg ctggaaaaat ggttcagata aagtagttat aataaatgga gatgtaagta
      661 tagatggcat tatatcaact ccactggcaa ctacatataa tgcgccaata cttttggttg
      721 aaaaaaacaa tgtgcctaat agtgtaaaat cagaattaaa gcgcctaaac cctaaagata
      781 taattataat tggagatgag aatgctattt ctaaaactac tgctaatcaa attaaatcaa
      841 ctgtaaatgc tagtcaaaca cgtttaaatg gttctaatag atatgagaca tctttattga
      901 tagcaaagga aatagataaa aatcatgatg tggaaaaagt atacataaca aatgctaatg
      961 gcggagaagt ggatgcactt actatagcag caaaagcagg tcaagacaag caaccaatta
     1021 tattaactga taaagatagt attacagaca atacatataa atggttaaag agtgaggatt
     1081 tacaaaatgc ttattttata ggtggtcctc aaatgatatc aacaaatgtt ataaataagg
     1141 taaatggaat aactaaggat agtgttacta ataatagagt atacggagca gatagacacg
     1201 aaacaaatgc aaacgtaata aaaaaattct atacagctga tgagttagag gctgttttag
     1261 tagctaaatc agatgtactt gttgatgctt tagcagcagg tccattggct gcgaacttaa
     1321 aatctccaat acttataaca ccaaagacgt atgtatctgc ataccataaa ggtaatttag
     1381 aagctaaatc agctaataaa gtatacaaaa taggaggagg attgacttct aaggtaatga
     1441 gctctatagc atcatcatta tctaaacaca atacgactcc aacagaacca ggaaatagtg
     1501 ggggcaagac agttatgatt gacccagggc atggtggttc agcacctgga aattcatctg
     1561 gaggaatgat tgaaaaagat tacaatttaa atacttcact tgcaacaact gaacatttac
     1621 gttcaaaggg attcaatgta ataatgacaa gagacacaga taagacttta tctcttggaa
     1681 atagaactgc tctatctaat tcattgaaac cagatttatt tacaagtata cattataatg
     1741 gctcaactaa taaacaaggt catggtgtag aagtatttta taagcttaaa gataaaaatg
     1801 gagggactac taaaactgta gctaccaata tattaaatag aattttagag aaatttaaac
     1861 ttacaaatag aggtataaaa acaagagtac ttcctagtga ttctacaaaa gattatttat
     1921 acgttttaag aagtaatgat atgccagctg tacttgtaga atgtgcattt ttggataatg
     1981 aaaatgatat gagtttaata aactcatctg caaaagtaaa agaaatgggt acacaaatag
     2041 gtaaaggaat agaagattca ttaaaataaa tggaaataaa aatatttaaa aacttaatgt
     2101 aaatttatag ttgtaaattt atactaaagt caaaaaatga aatataagtg tatcaatagt
     2161 aagactgtat gtacatttgc acatgtgaat gcaagtatat atacagtctt tttttgtagt
     2221 taaatgcaat aatttgattg tgttttgtaa aatacacaat atgatataat tagataatat
     2281 acacttaatt taattgagga aggtgattat atggaatatt tatttgaaaa aagtagctta
     2341 aaagaatatg gaaatgaagc tttagataaa tatagagaat tattagataa aactggtaat
     2401 aattcaaggt gtattctttt attagtacca aacaataaca caaagatacg atatgaaagg
     2461 gctctaagat taaattatag tgaagaactg aaaataacaa catatataag ttttgtgaag
     2521 aaagaattag ttaaatactg gccactaata atagaaaaat gtgatggaat aagtacaaag
     2581 gctgtatctc caacatttat atcaaatagt ttaagtgaat atttaatagt gcaaaaagtt
     2641 aaggaaaaaa ggatacaaga aggatatttt gaagatatta caggaaccaa taaatccata
     2701 gcaaatagta taatgactaa cataaataag tctgcattta atttaattga ttttgatttt
     2761 attggagaaa aaatatattc atcaaagaaa aataaagata gtatatatag attttcatac
     2821 actcaaatgg atgaaattat atcatattat ataaattcct tattatcaaa ttcaatttta
     2881 gataatgcat taagcatata tttatacaat caatacctac ttaatgatga tttttattta
     2941 aaaaccttat ttacagaagt gagatatata attgtggata gccttgaaag ctcaagtaat
     3001 gcagaagttg attttataac agaagcattg gataatacat tacaaagtta tatatatttt
     3061 gattatagta gggattattc tgtatttaat aatgtggata tagactatat aaatgagaaa
     3121 ataatatcaa aaattaagcc taaagaagaa aatattaaaa agaaggagat tggaatagaa
     3181 gatttatact tattacctgc aaatatagaa ttaaatcaat caagtcaatt atacaatgaa
     3241 atgttagatt taataagtga aaaagttata tctcttatag aagaagatgt atctccaaga
     3301 gatatagcaa ttatatctcc tataaacaat agtatattgg agcatcaaat tagagattct
     3361 ttgatagaaa aaaatataga tgtttttaat actaagaagg ataaaaaagc tgtagattat
     3421 ccatatggaa atgcattggt agtggctaca tgtatattct atggatattt agattttata
     3481 aaagatgatg aatacttaag ctttttagaa actcttttag aaataaacag gataaaagca
     3541 tttaagattt ttaatgaaac aagacattta agtattgata atatagatga acatagtcag
     3601 tatcgtgata ttttacagta tattgaagaa aaaaagaaga gtgatataaa aatacatgaa
     3661 tttttaactc aattttacat agataaaatg ttaaatttaa aagaaggtaa aaagaatgtt
     3721 gggttatgta aaaagataat agtcgaaagt gagtctttta gtgaaagttt aagtctactt
     3781 gggatgaaaa aagaaaagat atttatagaa gctttaaaaa gtacaataaa cgattattac
     3841 tctgttgtcg atatagaaga gttgaaatcc aaagacaaaa tagtaataac tactccttat
     3901 tcatatatct catcagatat agatagaagt atacaaatat gggtagatat aggaagtaat
     3961 gcttggaata tgaagataga aaaagatata agtaatttaa ttgtacttag aaaatctttt
     4021 gaagaaaaaa agatttatac aaatgaaatg gaag
//
LOCUS       DAEMAE010000267         3994 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEMAE010000267 DAEMAE010000000
VERSION     DAEMAE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.271"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            41..802
                     /locus_tag="KPH93_003371"
     CDS             41..802
                     /locus_tag="KPH93_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG1690143.1"
                     /translation="MKNKIETLMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHVMPKDFLKNGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHIAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1111..1389
                     /locus_tag="KPH93_003372"
     CDS             1111..1389
                     /locus_tag="KPH93_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690144.1"
                     /translation="MSYKKAIHVLPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELAIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            1682..2257
                     /locus_tag="KPH93_003373"
     CDS             1682..2257
                     /locus_tag="KPH93_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG1690145.1"
                     /translation="MQKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRINAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPSHLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSESGVDLKKGKLNYEIILDIGKEKQKSSN"
     gene            complement(2364..3197)
                     /locus_tag="KPH93_003374"
     CDS             complement(2364..3197)
                     /locus_tag="KPH93_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG1690146.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(3271..3894)
                     /locus_tag="KPH93_003375"
     CDS             complement(3271..3894)
                     /locus_tag="KPH93_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG1690147.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAIDAILNKIN"
BASE COUNT         1491 a          480 c          615 g         1408 t
ORIGIN      
        1 acattaaatt ggaaatatag aattatctag gggtgatact atgaaaaata agattgaaac
       61 attaatgaac aactttgtaa aaaattatca taaatcaaat aatataacta caaagtggca
      121 agaactttta tttgcttatg cctctgctga agatgatatg tttcagaagc taaaaagtgt
      181 tgtaggaaca tctcatgtta tgccaaaaga ttttctgaaa aatgggaaaa ctataattac
      241 atattttata ccttttgatg aaagtgtaaa taatagtaat attggagggt ttgaatgttc
      301 tgaaatatgg gcaagagcgt acatagaaac taatatgttg atttcaaatt taaatgattt
      361 tttatgtgaa gagctagaga agataggggc taattcatat gctattcctg ggtcgtataa
      421 ttttgacagt gaaaaattgg tcagtgattg gtcacaaaga catatagcat tcatagcagg
      481 tcttggaact tttggtttaa ataatatgct tattacagaa aaagggtgtt gtggaagaat
      541 aggaagcata atcacaagtt tagaattaga gccgactaaa agaccagaaa atgaatactg
      601 cttgtataaa cataataata gttgcaaaaa atgtgtcaat aaatgcgtaa aagaagcttt
      661 aaaagaagat ggatttaata gaaataagtg ttatgaaatg tgtatgataa atgaaaaaaa
      721 atacacttat ataggtggat gtgatatttg tggaaagtgt atagttggag ttccttgttc
      781 agtaataaac ccatcaagat aatggattta agttgactaa taatttaaaa tatagtttat
      841 aatgaaaaaa ttttagttat caaaataaat ataatataaa aactaaataa atgtgtttaa
      901 aaaatggata aataatttgt gttgattgac aaccagttat actgttgata gacttatatg
      961 tatcgctgtg atatataagg agggttgaga ttttatgttt gatttaatct ttatttttgc
     1021 atagcaaggg ctattgcaac tatgaaattt aatatataaa tagccctttg ctattcctaa
     1081 tgaaaaatta ctatttaata ggagagcaaa atgagctata aaaaagcaat acatgtatta
     1141 ccagaagaat tacttgaatt aatacaggaa tatgtagatg gtgaatgtat ctatattcct
     1201 agaaaatcta ataataaaaa agaatgggga agtaataccc ctactcgtga agagttggca
     1261 attagaaata tgcagattta cgaagactat caagttggat atgatttgca atatttatcg
     1321 gaaaagtatt atttatcttt gaaaagtatc caaagaattg tattacaaga aaaaagaaaa
     1381 gatatctaag gatgagccag tatagtttta taattatact ggctcatttt cttttgataa
     1441 aagtgtttga ggtatagttt cttttaaggg gatgatataa tttaagtaat ttcaatgact
     1501 tatacagtag ctaacaaagt tggttgtaaa gtacctagta agaagtaata gcttattata
     1561 tgcttgaaat taaaatttat taaaattagg aggtcttgtt ttgaaaaaaa tgcttgatta
     1621 tattccacct tttcataact ttctattagt ttagcaatct tatagaaagg aaattgtatt
     1681 tatgcaaaaa attacttggg aggtggaata tatctcccag ttacctgtaa ttaggtattg
     1741 taagaaatgt ggaaaaaaga ctgaatatat ttgttcaggt ttatttagga ttaatgctca
     1801 acataaatat ttggatatat ggttaatata taagtgttca aactgtgatt caacatggaa
     1861 ctcaactata tattctcgtg tcaatccaaa aagcttaaat ccagaaacac tagaacaatt
     1921 ccatacaaac gattataatc ttgctaagca atatgctatg aatatggaac ttttacatag
     1981 aaatggagca gaagttggta ttccagagta taagatagta ggacaagaaa ttaacattaa
     2041 tgaccttaca caagttcata ttattagtaa atatccatct catttaaagg tgtcaacatt
     2101 attacgtgag aaactaggtt tatctaggaa ggtatttgac gaaatgttag attgtggaat
     2161 tattagaagt gaatcaggag ttgatttaaa gaaaggtaaa ttaaattatg aaattatttt
     2221 agatataggc aaggagaagc aaaagtcttc aaattgatta gaataactaa cttaatatat
     2281 tttcaggaac atcattttaa aaaaatagtg actttaaaaa atttaaagcc actatttttt
     2341 aatatgtttt atctatataa ctattatttt tctaggtttt ttaatatttc gtaatagtgt
     2401 gttgtggcta tttcacttga ttcgcctttt ttacaaaaag ataatttaga tagttttgga
     2461 actacacgtt caacgacaat tttattacct atcattttag attcaagtat cttcaatgct
     2521 tctaaaaacc tcttatcctt ttttgcacga ttataaaatg ataaaacgta gacataaagg
     2581 aataggttat aattacggaa aggatattca acttgcataa ataaggttcc aattccatag
     2641 tgacaagggc cgattggttt tttaatagtc caatggtcaa gtagaaaatc tacagctttg
     2701 tcaagttttg attctttatt tagataatta ctaaaacgga atgcatttaa tatattaaga
     2761 gttggtagtg gatttgagta ttcagtttct ggacctcgac caaaactaaa tttattgcaa
     2821 cgccacccac catcagtata ctgtgtatct aaaaaatgtt gaaacgtttt ttgcattcta
     2881 atgtcagatg catatcccat atgacagagt acgttagcag catttgcagt atgacaaggg
     2941 taaatacctc catttggata tagtttaaaa cgtccatctt ctcgccatgt gctaaatatc
     3001 aagtcagcac attctttaag taaagtatca tcagggttca cacctaattc taatagataa
     3061 agtacactat caagagtaga gaaaggagaa ccttttatta aacgcttatc aggtgtagtc
     3121 caatagtctg caccattatc atacctgtgt gataatatta tttcaatatc tgataaagat
     3181 tgattttcta cattcatttt ttaccccatt tcatattata ttctattaaa tctatcaaaa
     3241 actttattat aatattattt atatttataa ttaatttatt ttatttaaaa ttgcatctat
     3301 agctgtatca ggaacaactg ctaaatcttc tgcattgaag taataaacta tattagaatc
     3361 aaataatata tttgaatttg gaggaaattc atcatctcca aaccaaagta taaataccat
     3421 agatgtatta cccatatatg taaacctata agctaaatct cccatgttta ctttttcagc
     3481 tccaattttc tccataacta acttatattt gtctatttga gatgtaaaaa cttctgaaag
     3541 cctatataga gtcctgttta aaaaatttgg ataataaaca ttgcctcctg gtatttcttt
     3601 aaaagttaca tatttattag ttctaggaat acctttagca ttgattaaat atctaactat
     3661 caagatttta agctcaaagt tattgcaaag gctaccatta ctattccaaa cttctccact
     3721 tggatagtct ataatatatt gtctatcaaa aaaattgagt gtgaatctat ttaaatcatt
     3781 attgaaaggt atattactta aattactaat tgtatgaggg tctaattctt tgagtttttt
     3841 tcgaacatag tcaaatggaa cattataatt attttttgtt atcttttcat ccataatttt
     3901 ttcctccaat ttaaacaatt aaaagtatat ttataaaaag ttttcttatt attaatttta
     3961 ttatatattc aaaatattac aataataaat tgtt
//
LOCUS       DAEMAE010000268         3977 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEMAE010000268 DAEMAE010000000
VERSION     DAEMAE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.272"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(172..3837)
                     /locus_tag="KPH93_003376"
     CDS             complement(172..3837)
                     /locus_tag="KPH93_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690148.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     VEGDTNAGNAEGDANAGDAEGDTNAGNAEGDTNAGNAEGDTNAGNAEGDANAGNAEGD
                     TNAGNAEGDTNAGNAEGDTNAGNAEGDINAGDAEGDTNAGDAEEDVNEVIVEEDAIAE
                     ESMSAGDFKISGGEIDVDFKFASGVLTILSGKQMTIQNNNPNTPTNQRIVVPKDVKAE
                     LIFAGVNIKATCPFTLTPDKDGGAYAHIILQDDSKNILTSSNIYYPGMRAGKSTTLII
                     DDSVVNRDIEGEFIVPENGKIPRDVTLENGDTLKKGDRLTLLDSKNRGSLTVVGNTHS
                     SGIGGGDTESGGNIIINGGIINATALGGSQSAGYGAGIGGGGNGTGGDITINGGTVTA
                     RGGYHGAGIGGGYYLSLKGVLSPAVVDPNTGQGYSGNITINGGLSYSYGGAHGSAFGD
                     GCVNSGTNRGYRVLVTGGTILPYMTGSGSWIFDLSATTGEVIVTGGSLKATTFKSFNG
                     AVAYGDLEKKTKVFMNKISLTYLGQDKVNTTLVDDFKMLINGKIYPYGAPSYTDAQGI
                     LYLWFSDQFKGSEVSVDLSVIDKITGEILKPDDFYIKDIGSGSQFLKQYEEFTIDDTP
                     PYLINKRYDGLPFEEDVEKGFLDWILKDGGVPVTTPKGEKLTDKKFMTISSQRLNDET
                     MEPDTNLGSNDLTKQIDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADAET
                     SLVVEESKVKTDVFKPTDAFTLNATVSPDKKEGPDCASPRGYVQFYINGKEYKDPVEL
                     KTHDKGSESHNYSTASIEWRPLDSNHHVLTTEQTITVKYIGDDINYIEGSEDEKKLKL
                     DMTNVDVDGDGKPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNG
                     DGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDIGSGGYDTGNPDP
                     NKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNGKPDPDSG
                     GNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDID
                     IDTDGDGKPDIDIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPD
                     VNIDIDGDGKPDINIDTDGDGKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFI
                     SLFFLIKNLTNKYLRRK"
BASE COUNT         1089 a          890 c          451 g         1547 t
ORIGIN      
        1 ttccattaat ctcttttaat aaaaaattct cttttctata tgaaaattat ttcaaaactt
       61 taaagttaaa tacaaaaatg actataaata gatttttatt tacacaaatc tatctatagt
      121 cactatagtt tatccaataa aataataaaa taaatatgta cacaataaac gttatttacg
      181 tcttaagtat ttatttgtta gatttttaat taaaaaaaac aagctaatga acattaatgc
      241 taaatctagc attatattgc tttgatctcc tgtttgtaca ttttgaccta cattactatc
      301 taaactttca tctgtatcta tatttatatc tggttttcca tccccatctg tatctatatt
      361 tatatccggt tttccatctc catctatatc tatatttaca tccggctttc catctccatc
      421 tatatctata tttatatccg gtttcccatc cccatctata tctatattta tatccggttt
      481 tccatctcca tctgtatcta tatttatatc tggttttcca tctccatctg tatctatatc
      541 tatgtctggt tttccatctc catctgtatc tatatctatg tctggtttgc tatttcctcc
      601 attatctgga tccggtttgc tatttcctcc attatctgga tctggtttgc catttccgcc
      661 attatctgga tccggtttgc tatttcctcc attatctgga tccggtttgc tatttcctcc
      721 attacctgaa tccggtttgc tatttcctcc actatctgga tctggtttgc catttccgcc
      781 attatctgga tccggtttgc tatttcctcc gttacctgaa tctggtttgc tatttcctcc
      841 attacctgaa tctggtttgc tatttcctcc attacctgaa tccggtttgc tatttcctcc
      901 gttacctgaa tctggtttat ttggatctgg attacctgta tcataacctc ctgaaccaat
      961 atctacattt ttatcaggct tccaataatc atctactcca tcgttatctg tatctattgc
     1021 tggacgatta taatctgtat cagggtttcc atctccattg gtatctatat ttttatcagg
     1081 tttccaaata ccatctccat ttccaccttc tgtactaggt ttccattcac ctgtattatc
     1141 agtatctatg ttaatatctg gcttccaatc accactcgta tctatattga tatctggcct
     1201 tccatcccca tcagtgtcta tatttatatc tggttttcca tcaccatcta catctacatt
     1261 tgtcatatct aactttaact tcttttcatc ctcactacct tcaatgtaat ttatatcatc
     1321 tccaatatat tttacagtaa tagtttgttc agtagtaaga acatgatgat tactatctaa
     1381 aggtctccat tcaatagatg cagtactata attatgactc tcactaccct tatcatgagt
     1441 ttttaattct actggatctt tatattcctt tccattaata tagaattgta cataacctct
     1501 tggagaggca caatctggtc cttctttctt atccggtgaa acagtagcat ttaatgtaaa
     1561 cgcatctgtc ggtttaaaaa catcagtttt aactttagac tcttctacaa ctaatgaagt
     1621 ttctgcatca gcaggaataa tttctgcctt ataatatgct ctatgccccc aataagcatc
     1681 cttaaaagga tatatactcg aaaactgtgt tgatgtaatg cttaattgat attttccagc
     1741 atcaatttgt tttgtaaggt cattacttcc taaatttgta tctggttcca tagtttcatc
     1801 atttaaacgt tgagaactaa tagtcataaa ttttttatct gtgagttttt cacctttagg
     1861 agtagttact ggaaccccac catcttttaa aatccaatct aaaaatcctt tttctacatc
     1921 ttcttcaaat ggtaatccat catatctttt attaatcaaa tatggtggag tatcatcaat
     1981 agtaaattct tcatattgct ttaaaaattg agaacctgaa cctatatctt taatataaaa
     2041 atcatctggt tttagaattt ccccagtaat tttatctata acagataaat caacagatac
     2101 ttcagatccc ttaaattgat cagaaaacca taagtaaaga atcccttgag catctgtata
     2161 actgggtgct ccataaggat aaatttttcc attaattaac attttaaaat catcaactaa
     2221 tgtagtatta actttatctt gccctaaata cgttagagat attttattca taaaaacttt
     2281 tgttttcttt tctaaatcac cgtatgcaac tgctccattg aaacttttga atgttgttgc
     2341 ttttaatgaa ccacctgtta ctataacctc tcctgttgta gcacttaggt caaaaatcca
     2401 acttcctgat cctgtcatat aaggtaaaat cgttcctcca gttacaagta cacgatatcc
     2461 tctatttgtt cctgaattaa cacatccatc tccaaatgca ctaccatgtg cccctccata
     2521 tgaataggaa agtccaccat taattgtaat atttccacta taaccctgac cagtattagg
     2581 atctactact gctggactta aaacgccttt caatgagaga tagtaccctc cacctattcc
     2641 agctccatga tatccgcctc tagcagttac agtaccacca ttgattgtga tatctccacc
     2701 agtaccgtta ccacctccac ctattccagc tccatatcct gcactctgac tccctcccag
     2761 agctgtagca tttataatac caccattaat gataatattt cctccacttt cagtatctcc
     2821 accaccaata cctgaagaat gagtatttcc gacaactgtt aaactcccac gatttttact
     2881 atccagtagt gtaaggcgat ctcctttttt tagcgtgtct ccattttcta gagttacatc
     2941 tctaggaatt tttccattct caggaactat aaactctcct tcaatatctc tatttacaac
     3001 agaatcatca ataattagag tagtactttt tcctgctctc atacctggat aatatatatt
     3061 agatgacgtt aatatgtttt tactatcatc ttgtaatata atatgtgcat atgcaccacc
     3121 atctttatct ggtgttaaag taaatgggca tgtggcttta atatttactc cagcaaatat
     3181 taattcggct tttacatctt taggcacaac aatcctttgg tttgttggag tatttggatt
     3241 attattttga attgtcattt gttttccact aagaatagtt aaaacgccac ttgcaaattt
     3301 aaaatcaaca tctatttctc caccactaat tttaaaatct cctgcagaca tagactcttc
     3361 tgcaatggca tcttcttcta caattacttc attaacatct tcttctgcat ctcctgcatt
     3421 agtatctcct tctgcatctc ctgcattaat atctccttct gcatttcctg cattagtatc
     3481 tccttctgca tttcctgcat tagtatctcc ttctgcattt cctgcattag tatctccttc
     3541 tgcatttcct gcattagcat ctccttctgc atttcctgca ttagtatctc cttctgcatt
     3601 tcctgcatta gtatctcctt ctgcatttcc tgcattagta tctccttctg catctcctgc
     3661 attagcatct ccttctgcat ttcctgcatt agtatctcct tctacatttc ctgcattagt
     3721 actttcttct atagtatctg cgaaagtatt tgaagcatta aatgatgcta ctgatccgag
     3781 tcccattgag atacttaaaa tcattgatag tgctttcttt ttacattttt gttgcataaa
     3841 ttccccctcc ccctaataat ttttaatata catcgtatac atccttatat atactctaat
     3901 aattcttaat atatgctgta tacattctaa tacatactct aataattttt aatatatact
     3961 taaaacttat atttgtc
//
LOCUS       DAEMAE010000269         3961 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEMAE010000269 DAEMAE010000000
VERSION     DAEMAE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.273"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            477..1061
                     /locus_tag="KPH93_003377"
     CDS             477..1061
                     /locus_tag="KPH93_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690149.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
     gene            1101..1904
                     /gene="proC"
                     /locus_tag="KPH93_003378"
     CDS             1101..1904
                     /gene="proC"
                     /locus_tag="KPH93_003378"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG1690150.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQETLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDLIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNISNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKILEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(1985..3160)
                     /locus_tag="KPH93_003379"
     CDS             complement(1985..3160)
                     /locus_tag="KPH93_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG1690151.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            3427..3903
                     /locus_tag="KPH93_003380"
     CDS             3427..3903
                     /locus_tag="KPH93_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG1690152.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
BASE COUNT         1482 a          484 c          653 g         1342 t
ORIGIN      
        1 aaatacttca ataattcttg acaatctaaa aaatgacaag gctgtatata aaaaatatat
       61 atagtgttaa actctaaaat aaaatatgtt gtagagtaaa aaaagacatt gaatagatat
      121 cattaaattg taaaattatg ttataattgt actaaaggat gtaataatgt gtagaaataa
      181 taatataatt tgaaatttta ataattaaac cagaaaaaga ctgcatgaac aacttaagtt
      241 ttattatata aagagtaaat gcttataaaa aatgtatctt tagttggcga aattaaatat
      301 acatatgtga aaaataaatc gtaaataatc gtaaaaatgt agacaaaaca agattgataa
      361 attatagtaa tacttataaa aattttttta agtgaaataa agataggttc agtacctata
      421 tttatatttt tgtatttata ttatgaaaaa attaaaatta tttaggggtg atggttttga
      481 ataaggacat aggagcaaaa ataaagcaac tcagaactca aaagcaaatg acgttgaagg
      541 atatgagtga aaaaacaaac ttgtcaattg gttttttatc acaacttgaa agaggattaa
      601 caagtgtggc aacagattcc ttaggaaaaa tagcaagtgt actagatgta gaattaactt
      661 acttttttat gaagccaaaa gagcataaaa gagctgtatt aagaagttat gaaaaagaag
      721 tatttgatgt agaaaactca acatttatac attatcattt atcatctagt ttaaaggaaa
      781 aaacaatgct accgagatta attgaaattc ttccaagtaa gagcagtgag gaaatttgtt
      841 gttatgtaca tgaaggtgag gagtttgtgt atgtattaga gggtactctt acagtgtttt
      901 tgggagatga gcaaatagaa atgtatccag gagacacaat acactataat agtgaaaaga
      961 acaatcataa ttgggtcaac tatactaata aggtagtgaa aattttggta gtaagtatac
     1021 caaatccatt tgaaaagtct gatgcagtta aagaagctta aattttaacg attactatta
     1081 gttaataagg aggtactaaa gtgaaaaaaa ttggattcat aggtgcagga aatatggcaa
     1141 gcgctatgat agggggaata gtaaattcaa aattagttga acctaatatg gttatagcct
     1201 cagcatatag tcaagaaact cttgatagaa tagatgcaaa ttttggaata aatactacaa
     1261 aagactcaaa ggaagtaact agaacctctg atatagtaat tgtggctgtt aaaccagata
     1321 tatatgatga tattttggaa gagataaaag accttataga tgacaataaa attatagtta
     1381 caatagcagc aggaaaatct ataaaggata tagagtctat tataggagaa gataaaaaaa
     1441 tagttagaac tatgccaaat actccagctc ttgtaaatga agccatgtct tctctttcta
     1501 taaataaaaa tataagcaat gaagacttag aagcagttac agaagtattt aattcttttg
     1561 gaaatactga ggtagtacca gagtatctta tagaagctgt aattggtgca agtggttctg
     1621 caccagcata tgtattttta tttatagaag ctatagcaga tgcagcagta attgcaggaa
     1681 tgccaagacc acaagcatat aagtttgcat cacaggctgt tatgggttct gcaaaaatgg
     1741 tacttgagac aggaaaacat cctggtgaat taaaagatat ggtatgttca cctggtggaa
     1801 ctactataga agcagttaaa atacttgaag aagaaggttt tagagcttct gtgataaaag
     1861 caatatgtgc atgtatagaa aaatctaaaa aaatgagtga gtaataaaaa tttattgtaa
     1921 ataaagagaa ttgcctttta tagaaaattt ccattttagg caattctttt tatttgttta
     1981 atttctaaaa taagcctgaa agccaatttt ttattttttc aaagaaacca actgagtcat
     2041 tattatttga ttgattttct gaattatttg ttgtgttgtt ttcatttgtt tgagaactat
     2101 cgttattttc attattactt gaagtatcat tttttgagct atttgtatca ctatcattgt
     2161 tagaattagt attattttgt ggatttattt gaacaccttc tgtagaattt attacagcat
     2221 cagagccaag agactcatca ttagtgttat ttaagattcc caaatctttt ttatctccgc
     2281 ctgagaataa accagagaac cagtctccta ttccactaaa ccatccacca atagtatcta
     2341 gtatgccaga acctttaaca ctttcaccta atttctttaa ttgttcattt acattatctg
     2401 atacattatc tagtgtattt ttcatgctat tataatcgta attttggtca gctatttttt
     2461 tcattacacc tttaatttgt tctttttgtt ctggtgtaat agtaatattg taattattta
     2521 caacattatt tattgtattt gctatttgat tggtatcttt tgtaccattc tttacaattt
     2581 ctgtttttat atcattcatt acacctgttg ctttttcttg ccctatatca tctcctaagt
     2641 cacccgtaac taccaattct tcagaagcaa tttctttctt agtttcatca agtttctttc
     2701 ctgaagcatc ctcaaatgct ttcatgattc ctgtcaatgc tccagtacca cttacaggat
     2761 atggagcagc agcaataaca tttgcatctt caagaccagc tgttgtaagt gttgaagcaa
     2821 tcattgaact agttacccaa gttaagtttg ctgttttaac attaattcca tttccttttt
     2881 tagtgggttc aacgtatgag caagagatag taacttttcc aagttgaccc tctgttgcaa
     2941 caccttgtaa atactgtctt tcttctgcat ttgttacatc taaaactaag acttggtctt
     3001 tttttactcc aaagtaatta agcatttgct gcttttgaga ctctgataag ttagctccaa
     3061 gtgtaactac atttgtaccg tctgcataag aaagattcat actcccaagt acaagcatag
     3121 tagacacaac aatagaacta atttgttttt taaacttcat aatattcctc ctagatttaa
     3181 catacattac attttcaatc tattttttta ttaatatatt gaataaaaat atgtgctaca
     3241 tttctattat actaaatata aactattttt taaatcaatg cgattacaac tttgtaataa
     3301 aaaagggaat ataaggaaga taataaaaaa ataatttttg taagagattg aaaaaacaat
     3361 taatgtagta tactataata tagaaatgat tatgataatg ttatagaatt ggaaggagaa
     3421 aaataaatgt caaaagaatt agtaaaagat agagtaataa aatatttaat agaagattta
     3481 ttagtacctc aagatatgat tgatacaaat gttgagttgg cagaatttga agaaggtgca
     3541 gaaggaattt tagatattgt tgtaaatgtt aaagatgaag aagattatta tgcacctgta
     3601 atgatagtgc aatgtttaga tgaagatgtg gagttagaag gagaagtttt acaaaaacaa
     3661 atagaatttt tagaagaagt ggataatata actatgtcag gtagattggt tttaactaat
     3721 ggagatgcta tgatgtacgc tgattggaga ggggaagaat acgatactga ggcagctctt
     3781 ccaacttatg atataatggt aaaagagttt catgaaatgg aacaacaggt aaaagattta
     3841 gaagaacatc atcaccatga tgaaaattgt ggatgtggat gtaatcatca tcatgaaaat
     3901 taaatattga gcatatatat aaaaaaagac tgtttagtaa acagtctttt tttagacata
     3961 t
//
LOCUS       DAEMAE010000270         3939 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEMAE010000270 DAEMAE010000000
VERSION     DAEMAE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.274"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..540
                     /locus_tag="KPH93_003381"
     CDS             106..540
                     /locus_tag="KPH93_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690153.1"
                     /translation="MKILKKITSIIFLIIMITVVITGCSQQEQGKNNSEKKLNYNVLG
                     EKTRHGGTYGDFKFVVIEMPKDFDPSNCKNLSKDFLNYYNENVDTITVYVYEKGYKLE
                     SEQDMYKNSLYDITIEHRDENTCVYSVWDVKSEETTDEGSLN"
     gene            complement(628..2187)
                     /locus_tag="KPH93_003382"
                     /pseudo
     CDS             complement(628..2187)
                     /locus_tag="KPH93_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430345.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            2392..2832
                     /locus_tag="KPH93_003383"
     CDS             2392..2832
                     /locus_tag="KPH93_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBG1690154.1"
                     /translation="MRGVIIIKGGVRKKGKKWYYYFDLGVIDGKRKKVERAGGNTKKD
                     AEKALREALKEYENTGIMFDECEMNLAEYLDFWFNKYVILNCKYNTQESYRIHIQTHI
                     KPALGHYKLKSLTPATLQNFINAKFRSNYSQSTLEVIRAILKKL"
     gene            2859..3533
                     /locus_tag="KPH93_003384"
     CDS             2859..3533
                     /locus_tag="KPH93_003384"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1690155.1"
                     /translation="MKDNPMQYIAIPKNKTIKKEIKVITLDEFNNILNLFNKGTYQYI
                     SLMVAFHTGMRKGEVAGLTWEDINLKTKTIDLYPPMIKKKKGDIELATPKSSSSYRTI
                     TIGDTLVKALKEHKKYQNENKLLFGEFYINNNFVCTKINGSPININSISTMCNGIIKK
                     INTHFTFHSLRHTHATMLLEAGANIKAIQKRLGHSNIAVTMNTYSHVTETMKQETVDL
                     FEKSIK"
BASE COUNT         1432 a          566 c          496 g         1445 t
ORIGIN      
        1 attgcatatt tttccaaaac acatagcatc tttgtagaaa attttatata cttacattat
       61 tactatcata aagtagaata aaaatatgtg agggaggttt attgaatgaa aatactaaaa
      121 aaaataacta gtataatttt tttaataata atgataacag ttgttattac aggatgtagc
      181 caacaagaac aggggaaaaa taattcagaa aagaaattaa attacaatgt tttaggagaa
      241 aaaacaagac atggtggtac atatggcgat tttaagtttg ttgttataga aatgcctaaa
      301 gactttgacc catcaaattg caaaaatttg tctaaggatt ttttaaacta ctataatgaa
      361 aatgttgata caataactgt ttatgtatac gaaaaaggtt acaaattaga aagcgaacaa
      421 gatatgtata aaaattcttt atatgatata actattgagc acagagatga aaatacttgt
      481 gtttattctg tttgggatgt taaatctgag gagacaacag atgaaggttc attaaattaa
      541 aacctttaac ttgtcttttt agaaataaaa gaggaactaa gttatattca tttttataac
      601 ttagctcctt atagtaaata tttattttta acttattcca tctacatcat tatcaaaagg
      661 attattttct gatggaacga atataatatt aacgtcatca acatctctat tataatgctt
      721 atctgcttct gtatttgggt ttacatcatg atatgatact aattcttcat tttgatttaa
      781 acgctgtata tcatgaacag tccatgtatc agtatctata aaaacccaat atgaatctat
      841 tccagaactc tcatacaata caactacata aaaatcagta cttatattat gttcttctct
      901 atctagtatc aaatcatttt cactaatatc atatttacta gaacatgctt caaatgccat
      961 atcgattgct tttctctctt ttaaatatcc ttccgcatca aaccttttta ttgtattatt
     1021 tgaataccat tgtagtatat cattcaaatt attcatattt actaaaaatc taaaatcatc
     1081 atcatctgca ccaattgcat aataatcttt cccttgatag gtaaagtttt ttatataata
     1141 cctaaaacat tcatccgggt ctgtttttgc tttaatagct gattcataat ctacaaatac
     1201 tttttcatct aaaatataag ctattctttc agttgcttcc tttacagtat acttccctcc
     1261 agtagttgga ctggcaatat tttttgttat gtttagagat tgttctatag cattgttatt
     1321 tagatttcct attgctgtta ttttagttgc accttttaat acagtcttat tactattgtc
     1381 atcaactaac acaattggag tatttttagc tattgtagag ccaacaagag catacactaa
     1441 atctgttcca cttgctatgt aaaactcctt tattccatta tagagcttat ttactatctt
     1501 tttattagta tcatatctgt ctgctccgcc caatcgagtt gaattagtat cattaactag
     1561 tttgtcactc attgatgaag taccacctat tgcataactt tctattccag tagtattaaa
     1621 tggtacactt ttcccatcag ttaatattat aggtgctttg tctctaacag ccacagaggc
     1681 aatactcatg gcatctggtt cacctttgaa agcattagtt aatattacct tatttacttt
     1741 attaatagaa tttatttctt ttgctacatt ataacttgtt ttaattctat catttccttg
     1801 aagtctttta gtctctattc ctttatcttt tagaaaagtt tctgttgcct tatcaataga
     1861 actttctcca ccaattatgt acactttgtt agctttctct actcttttaa gagttgcatt
     1921 aggtatatta tttttcttag tcaaaagaat tggtgcattt gtagctcctg caagcccact
     1981 tgcacttaac ccatccgcca ttgtactatc tgcatttata agtattgcag tagcataact
     2041 ttgtttgtct gcgataagtc cagcagtttc ttatttatct gctccttgaa tcttatcgat
     2101 tttatcaagt gcatttgcag atataggata acaagcaaca aacattgaca tagctaaccc
     2161 tagcgataat aatttctttg atttcacaat atctccccct ttttcatatc actcaattaa
     2221 attgtataac aaaaatatat tatatgttgt cgaaagtaca aactatttct acttaattaa
     2281 cgacattaaa taacattaga ataaattatt ttcttataat ctcattcttt ataatatata
     2341 taattgttat aatataatta aagcatttta ctttaaccac attatttaat tttgagagga
     2401 gttattatta ttaagggtgg agttagaaaa aaagggaaaa agtggtatta ttattttgat
     2461 ttaggagtta tagatggaaa gagaaaaaaa gtagaaagag ctgggggaaa tactaaaaaa
     2521 gatgctgaaa aagcacttcg agaagcattg aaagaatatg agaatacagg aataatgttt
     2581 gatgagtgtg aaatgaactt agcagaatat ctagattttt ggtttaataa atatgttatt
     2641 ttaaactgta aatataatac tcaagaaagt tatagaatac acattcaaac acatattaag
     2701 ccagcattag gacattataa attaaaatcc ctaacaccag ctacattaca aaactttatt
     2761 aatgctaagt ttagaagtaa ctattctcaa agtacacttg aagttattag ggctatttta
     2821 aaaaagcttt aaaatcagct gtctacccat atcagttttt gaaagataac ccaatgcaat
     2881 atatcgcaat tcctaaaaat aagactataa aaaaagaaat taaagtaatc acgttagatg
     2941 aatttaacaa tattttaaat ttatttaata aaggaactta tcaatatata tcattaatgg
     3001 ttgcttttca tactggaatg agaaaaggtg aagttgctgg tctaacttgg gaggatataa
     3061 atctaaaaac aaaaactata gatttatatc ctcctatgat aaaaaagaaa aaaggagata
     3121 ttgaattagc aacaccaaaa tcctctagtt cttatagaac aattactata ggtgatactt
     3181 tagtgaaagc tctaaaagaa cataaaaagt atcaaaatga aaataaattg ctatttggtg
     3241 aattttacat aaacaacaac ttcgtatgta caaaaataaa tggaagtcca ataaatatca
     3301 attctatttc tactatgtgc aatggtatta ttaaaaaaat aaatactcat tttacttttc
     3361 atagtttaag acatactcat gcaacaatgc ttctagaagc tggggcaaat ataaaagcaa
     3421 tacaaaaaag gttaggtcat agtaatatcg cagtaactat gaatacatac tctcatgtga
     3481 cagaaactat gaaacaagaa actgtagatt tatttgaaaa atccataaaa taaaattctt
     3541 ccaccgaaaa ataattacga tggaagaatg gtggaagact cctttaaaaa tgttatattt
     3601 atagtttcac ttatattcgt tcaactcact ttacctcttg gtattactgt attcaaacat
     3661 atactaaata taaatataat ttatctaaat aaatttttca acttaaatat ttcttaagat
     3721 ttaagataaa ttgaggttta tttgaataaa ttgatttgaa ttatgttatt ttcagattac
     3781 ttttatttaa gtattttgat tggattcagc tgaattaatg tatattaatt cttattgaac
     3841 agtttttatt tttatacata ctatctacta tatactatac atattctact aaaatttata
     3901 taaatattag acaaacctat ttcattttct taacatgtt
//
LOCUS       DAEMAE010000271         3890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEMAE010000271 DAEMAE010000000
VERSION     DAEMAE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.275"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            21..1328
                     /locus_tag="KPH93_003385"
     CDS             21..1328
                     /locus_tag="KPH93_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690156.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKAEIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLTSKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1414..1676
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1802..2605
                     /locus_tag="KPH93_003386"
     CDS             1802..2605
                     /locus_tag="KPH93_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1690157.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKKSKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2643..3254
                     /locus_tag="KPH93_003387"
     CDS             2643..3254
                     /locus_tag="KPH93_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG1690158.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3286..>3890
                     /locus_tag="KPH93_003388"
     CDS             3286..>3890
                     /locus_tag="KPH93_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG1690159.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMIKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVA"
BASE COUNT         1529 a          385 c          734 g         1242 t
ORIGIN      
        1 aagcaaaggt gggaataaaa atgaaagaaa ttgctgatgc tataacatca gagtatttaa
       61 gctatggtat caatgaagat attagtatct atgaaggtag attttgcata tatttagata
      121 aaaaatatag atgtaatggg aaaatttatt ataaaatgac acctccgata tctataagtt
      181 ttaaagcaga gataggttgt gttgaagaga tagacaatga agatgacaat ttagctttag
      241 attatgataa tgctattctt gaggttcatg gatataaaat tatttctata acaataaata
      301 ctttaagtga atttagtgta gaaggctata tcaatgatga ctgcataaaa tctaagaact
      361 cctatgttga atatgtagat tttaatataa taaacttaga taaaatacct ggaaaactca
      421 ttaaatacaa tgataaggta tacgcaggta gaattgaatt tgatataaat gattatgttg
      481 taactataga caagcgatat gattatagaa aagaattaaa atcagaatta aagtctaaat
      541 ctggagcaat aataactcat ataggtagga ttagaagaaa agatggtaga atttttagaa
      601 caaataatac aataaatctt ttagatagaa tatcaacagc tttgagtttt atgtgtggaa
      661 gatatgtagg gttttgtttg gccaaagggt atagaagtgg taatgaagtt tatagaatat
      721 ggaatgaaaa tcaaatatct ccttttagat atgtaccaac atggtcagat acactttcaa
      781 attatcataa tatggaaaaa tatataagcc ttatgtgtaa aaagctagaa gatttttatt
      841 atggttctgc tataaagagt gttgttgatt ggtacataga atctcttgga agtgctacca
      901 tggaaaacaa tataatatct gtacaaattg cacttgaaac tttgtcatat gtaattctag
      961 tagaacaaag taagatactt acagatgaag catttgactg taatttgaca tctaaaaata
     1021 taagactctt acttgataca tgtaagatac catatggtaa acatgaatta aacatatttg
     1081 ataatataat aaaaaataaa tttgatgatg gagtggattt agttatttat ttaagaaata
     1141 gcatagttca tccaagcaga aagactcata gagctgtgtt agaggttgaa gatatttgga
     1201 atataatttc aataggaacg agatatattg aacttgtttt actctttatt ttaggttata
     1261 gaggagaata ttcaaacaga ttagtagaaa ggtgttatgg tgaagtggaa gttgtacctt
     1321 ggaactagat ttttatattt attttaaaat atatattgac atatattgcc ttatgctata
     1381 aaatcaatat cataataata attcacaata acattaaatg ctaagaaaag aaatagtaaa
     1441 agtagttaat cttaaacaga gagtttccga ttggtgagag gagcaataga ttttactttg
     1501 aacacatctt tgagcaggac tctgaaagag tagaaatact ttattaggag atttccggaa
     1561 tccttgcacc cgttatagtg ctagagtata atgattttat tgtacttaaa gaggttagtg
     1621 tcgtgaggca ttaatgaaca gaggtggaac cacgagagtt aactcccgtc ctctagcaga
     1681 agctagaggc gggagttttt taatataatt ttttaagcgc gcagagaatt atattacaat
     1741 agatacactt tttaaaaaat aatcaaaata ttttaaaaat taaaaggcga gggagaatat
     1801 tatgaaaaat atattaaaaa aagtaggaat atttacaatt atgttaggtt tattaggtgg
     1861 agttgtaggt tgttcaaaac cagataatga aaaagataag gatgcttcaa agaaatctaa
     1921 aaaagaagta gttgtagggt ttgataatac ctttgtacca atgggatttt tagatgaaaa
     1981 gggagataca gtaggatttg atgtagactt agctaaagaa acatttaaaa ggcttggaat
     2041 ggaagttaag tttcagccta tagattggtc aatgaaggaa actgaattaa atgattcaaa
     2101 aactgtagat gtattatgga atggatattc tataacagac gaaagaaaga aaatagtatc
     2161 atatacagaa ccatatttac aaaataagca gataatagtt actctaagtg attcaaaaat
     2221 aaattcaaaa gctgatttaa aagataaaga agttggaaca caacaaggtt caacagcact
     2281 tgatgcagtg gaaaaggata aagattttat gaatagctta aaaggtggag caccagttct
     2341 ttatgatact tatgataaag cacttagaga tttagagata ggtagaacta gtgcagttgt
     2401 tggtgatgaa gttttaatta gatactatat gggacaaaaa ggtgaagaca aatacaaggt
     2461 attaaaagac gattttggat tagaagatta tgttgtagca acttctaaag aaaatccaga
     2521 actttgtgag aaaataaatg aaacattaaa agaaatgaaa aaagatggaa cttttgacaa
     2581 aatttatgat aagtggttta aatagaagat aagttagctg ttaagaaggg ggcactaaag
     2641 gcatgttaca aggtttagag atagttatag caatgttttg tataacattg atagtatcaa
     2701 ttccattggg gattggagtt gcatttttaa gattatcaaa gaataaatta gtaagtggta
     2761 ttacacaatg ttatatattg ataatgagag gtacaccttt acttttgcaa atgatagtca
     2821 tattttacgg attacctctt ttaggaattg tatttgacag atttacagct ggagtagtag
     2881 ctttcttctt aaattatgca gcatattttg cagaaatatt tagaggtgga atacagtcaa
     2941 tcgatagagg acaatatgaa gcttcaaaag tactaggatt tgataaattt actatgtata
     3001 agagagtaat ctttccacaa gtttttaaaa gaatactagc acctatttca aatgaagtaa
     3061 tcacattagt aaaagataca tctttagtat atatattagg attaaatgat attttaagaa
     3121 tatctcaaat agcaatgaac agggaagcaa gtctgttacc attatttgag gcaggagcga
     3181 tatatcttat atttgtagct atacttacta agggatttga attacttgaa aaaaaatatt
     3241 cttattatag ataaagttac aatattatag gaggaggttt tttatatgtt gaaaatcaaa
     3301 aacttaaata aatcatttaa aaaaaataga gtacttaaag atatttcttt tgagttagaa
     3361 gaaggtcaga ttggagtttt acttggtaag tctggtgcag gaaagaccac aatattaaga
     3421 tgtataaatg gtcttgaaga atttgatagt ggagagatta taatagataa tgaggtaata
     3481 aaaaataaga gagatatgat aaaaatacga ggaaaaatag gtatggtttt tcaaaatttc
     3541 aacttatttc ctcatatgac agtccttgaa aatataattg aatccccagt taatgttttt
     3601 aaagttccta gaaaagaggc tgaagagagg gctagagaac ttttaagatt agttgattta
     3661 gaagataaat taaattctta tccatttgaa ttgtcaggag gacagcaaca gagagttgct
     3721 atagctaggt catgtgcatt gatgccaaaa gtcctttgtt ttgatgaacc tacttctgca
     3781 cttgatattg atactattca aagagtagtc aatataatga acagacttaa agacaaaggt
     3841 atgacaatat taataattac acatgatgtt gtcttctcaa ataatgttgc
//
LOCUS       DAEMAE010000272         3869 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEMAE010000272 DAEMAE010000000
VERSION     DAEMAE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.276"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..1037
                     /locus_tag="KPH93_003389"
     CDS             84..1037
                     /locus_tag="KPH93_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG1690160.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSVAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFMFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1027..1983
                     /locus_tag="KPH93_003390"
     CDS             1027..1983
                     /locus_tag="KPH93_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG1690161.1"
                     /translation="MKLSLNQKIYFISILLFISIALFVIYEIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGI
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1980..2735
                     /locus_tag="KPH93_003391"
     CDS             1980..2735
                     /locus_tag="KPH93_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1690162.1"
                     /translation="MIEIKNIFKKYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTEEDENCIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2827..3792
                     /locus_tag="KPH93_003392"
     CDS             2827..3792
                     /locus_tag="KPH93_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1690163.1"
                     /translation="MNKKAAIVAAIAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDEKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFEVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1552 a          371 c          662 g         1284 t
ORIGIN      
        1 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
       61 taaaagattt ttaaggaggg agaatgaaaa aaaggtattt agtcatagga ataataattt
      121 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcaa
      181 aaaaccagca taagcttatg atatttatca tgagtagagt tccgagatta ataagtatac
      241 tgattgctgg tgtagggatg agtgtagcag gtcttataat gcaacagata agtaaaaata
      301 aatttgtatc acctacaaca ggagctacaa tagatgcagc tcaatttggg atagtaatat
      361 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
      421 ctcttgtagg tacttttatg tttatgaaaa taataggaaa gttgcaattt aaaaatataa
      481 tctttgtacc attagtaggt ataatgtttg gaaatataat aggttctatg acggacttta
      541 tcgcatataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
      601 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
      661 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgca acaaacttag
      721 gtcttagtta caaaagaatt gtcaatatag gtttgataat agtagcactt gttacagtat
      781 gtgtagtcgt tacagctggt aatatccctt ttataggcct aatagttccc aatatcgtat
      841 ccttgtatat gggtgataat atacgagcaa gcatatggta tactggttta cttggagcga
      901 tatttgtact tatatgtgat atttttggaa gaatcattat ttatccatat gaaatatcta
      961 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
     1021 acgtaaatga agcttagtct caatcaaaaa atttacttta taagtatatt actattcata
     1081 tcaatagcac tgtttgtaat ttatgagata gacatgaatc atttagaata tgcattatca
     1141 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
     1201 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
     1261 tcattatatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
     1321 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
     1381 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
     1441 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaagt aatgatagac
     1501 ccgaatgaat ttttagcttt gcaaactagt ataatggcca gtttaaacaa tataaacact
     1561 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
     1621 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
     1681 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
     1741 gcacttattg gacctatgac atttttagga cttttacttg ccaatataac aagagaaatt
     1801 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gataattaca
     1861 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
     1921 gtaataaatt ttataggagg aatttacttt attcaattac tattaaaggg ggcaaataga
     1981 tgatagagat aaaaaatata tttaaaaaat ataagaataa aaatgttgta gatgatgtgt
     2041 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
     2101 gtacagtact atcaatagtt acaaggctta taggaggtga tggtggagaa gtaataatag
     2161 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
     2221 agcaatctaa caatataact ttaaaactta caataagaga attagttgga tttggaagat
     2281 ttccatatag tgaaggtaat ctaactgaag aggatgaaaa ttgtattgat gaagcaatag
     2341 aatatatgaa gttgactgat atacaacaca agtatttaga tgaattgagt ggaggtcaaa
     2401 ggcaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
     2461 aaccactgaa caatttggat atgaaccatt cagtacagat gatgaaggta cttagaggtc
     2521 tttgtgatga gttagataag actatagtat tagtaatgca tgatataaat tttgcatcat
     2581 gttattcaga taatatagtt gcattaaaga atggaaaagt tgaaaaagta ggtcgtacag
     2641 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
     2701 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatataaa aataagcttg
     2761 ctatatgatt ttttgtaaaa tttttaaatt aaaataaaga ttaataggag agaggaaaat
     2821 attgatatga ataaaaaagc agcaatagta gcagcaatag ctattatagg attagtgact
     2881 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
     2941 actataaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaggta
     3001 gtagtatttg actattcggc tttagataca atggatgcat taggtgtagc tgagaattta
     3061 gtaggacttc caaaagcgag tttaccagca tctttagaaa aatataagga tgagaagtat
     3121 gcagatttag gaggcttaaa agaaccagat ttagaaggta taaagtcagc aaatccagat
     3181 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
     3241 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagat
     3301 aaaatagcaa aaatatttga agtagaagaa aaagcaaatc aagaatttag taaaattgaa
     3361 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
     3421 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
     3481 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
     3541 attacttttg agtacatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
     3601 atagctactg gtagtgatgt aaaagaaagc tcaactgcaa agtctgtttt aaacaatgat
     3661 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
     3721 tggtatgtaa atgatggtgg tctgacatct ttaaataaaa tgatagatga tgcttcaaaa
     3781 gcagtaaatt aataatccga tataatcaaa atgtattttt tgtaaaatga ttatcaaata
     3841 aactaggagt ttttaagaac tcctagttt
//
LOCUS       DAEMAE010000273         3858 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEMAE010000273 DAEMAE010000000
VERSION     DAEMAE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.277"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            146..1801
                     /gene="ilvD"
                     /locus_tag="KPH93_003393"
     CDS             146..1801
                     /gene="ilvD"
                     /locus_tag="KPH93_003393"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG1690164.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNQYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFAKEINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIIFINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2147..2776
                     /locus_tag="KPH93_003394"
     CDS             2147..2776
                     /locus_tag="KPH93_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1690165.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLENLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPKELAEMLVFSI
                     DILLDPVLFKREYSEVCNKLDFLLFMLKKMDTPLIDECGIKKLKDLFKQ"
     gene            2802..3614
                     /locus_tag="KPH93_003395"
     CDS             2802..3614
                     /locus_tag="KPH93_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1690166.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKHYKVYVFSRKNQITEKYSTRDMAKDQSDIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNGLMIDT
                     ANKSYSERYLKKYRLFIPLLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1491 a          450 c          734 g         1183 t
ORIGIN      
        1 gattgtgtaa aatattgata aaaaatcgta ataaattttc attagtatat gctaatattt
       61 ttttacaatt ttcgtaatta attaatagaa aaatatatta taataatatt atgaaaattt
      121 aaaaaagtaa ggaagaggta atattatgag aagtgatata aaaaaaggaa tagaaggagc
      181 tccaaagaga gcgttaatgt atggaatggg tcttactaaa gaagaaattg aaagaccatt
      241 aataggtata gtaaatgcac aaaatgaggt gattcctggt catctgcatc ttgatgaaat
      301 agcagaagct gcaaaaaatg gagtaagaat gagtggtggt ttgccattag aatttcctgc
      361 tataggtgta tgtgatggca tagctatggg gcatgtcgga atgaattatt ccctggcttc
      421 aagagaatta atagcagatt caatagaagc tatggctatg gcacatggtt ttgatgcttt
      481 ggtacttata cctaattgtg acaagatagt gccaggtatg ttaatggctg ctgcaagact
      541 taatatacca agtattgttg tgagtggagg gcctatgctt ccaggtaaaa aaaatggaaa
      601 agtatacgat tttaactctg caatggaggg tgttggagca tgtaaagatg gaacagtaag
      661 tgaagaagaa ttagaagagt tagctatgaa ctcttgtcct ggatgtggtt catgttctgg
      721 acttttcaca gcaaatagca tgaattgttt aacagaagct ttaggcatgg gtataccata
      781 taatggtact gctgcgtcac attctggaga aagaaaaaga atagcaaaat atgcaggtat
      841 gtatgttatg gagttactta agaacgacat aaaacctaga gatattttaa caatagatgc
      901 ttttaaaaat gctatagctg tggatatggc aatggctggt tctacaaata cagtacttca
      961 cttacctgca atagcttatg aatcaggaat agagcttaac ttagattttt ttgatgaaat
     1021 aagtgaaaaa actccttgtt taacaaaatt aagtccaagt ggaaaacatc atattgaaga
     1081 tttacatatg gcaggaggaa taccagctat aatgaacgaa ctttcaaaga taaatggaat
     1141 aaatttagat tgcaaaaccg taacaggcaa gactataggc gaaaatataa gaaattgtga
     1201 aatagaaaat gaagaagtaa tacatacatt aaagaaccaa tatagtaacc aaggtgggct
     1261 tgcaatattg aaaggaaatc ttgctctaaa tggagctgtt gtaaaaaagt cagcagttgc
     1321 agaagagatg ttagttcatg aaggaccagc aagagttttt aattcagaag aagaagctgt
     1381 aaatgctatt tttgctaaag aaataaacaa aggtgatgtt atagttataa gatatgaagg
     1441 tccaaagggt ggtccaggaa tgaaagaaat gctatctcct acatcagcag ttgcaggaat
     1501 gggacttgat aagcatgtag cacttcttac tgatggtcgt ttttcaggag caactagagg
     1561 agcatctata ggccatattt ctccagaagc tatggaaggt ggtttaatcg gactagttga
     1621 agaaggagat ataattttta taaatatacc agacaaaaaa ttagagttaa aagtagatga
     1681 agttgaaata gaaaatagaa aattaaaatt taaaccttta gaaccgaaaa taaagcatgg
     1741 atacttaagt agatatgcta aattggtaac atcagcaaat acaggagcag ttttaaaata
     1801 gtggactata tttacgaaaa ttagaaaaag ttaagaaaat attaaatgga tatcttaggt
     1861 gttacagcac taatgagata tcctttttat tttgtatgaa aaacacagca aaatcttgat
     1921 tatctttgaa aacaatacat ctctattaaa ggaatatgag tacagcatag agatgtatct
     1981 tttcgaccct gtgaggtaag acaacttttt aaaagtttga tgtggaatgc ctttcattcc
     2041 ctctgaacta tgaagaaatt aaaagtcagt taattcttga cataataaat attaaaatgt
     2101 ataataaaaa aacaaacaga tagtctgttt ttttaagggg ggcaatatga atagagaaga
     2161 aaaaagtaaa aatagtaaag aaaaaattat tcaatcagca ttttcactat tttcgtctaa
     2221 aggatatgat tcaacatcta cacaagatat tatcaattta tctggtttat ctagaggtgc
     2281 aatgtatcat cactttaaaa ctaaagaaga tatacttaga agtgtcacaa aggaacttta
     2341 ctcacaaatg aataattttt tagaaaatct tgttgctgat gacacactta cagcaaatga
     2401 aaaaataata gaattggttg ttcatagtgc gaatgattac acacgtagaa aaatggtaca
     2461 ttgtagctgg ttagaaaaaa taccattcgc tttaatagag gaagttcgta atctcaacaa
     2521 tgtagttgca cccaatattg ctaagataat taaacaaggt gttgaaaata aagaattttc
     2581 ttgtgaatat ccaaaggaat tagctgaaat gctcgttttt agtattgata ttttacttga
     2641 ccctgtatta tttaaacgtg aatacagtga agtatgcaat aagttagatt ttttattgtt
     2701 tatgttaaaa aaaatggata ctcctctaat tgatgagtgt ggtattaaaa aacttaagga
     2761 tttatttaaa caatgataat aggaaaagtg aggaatagac tatgttttat aatgcaaaaa
     2821 gttgtcaaat gatgataggc agtaatacag tgaactatat tgaatttggt agtggtaaga
     2881 aaacattaat tattttgcca ggattaggag atggtttatt tcctttacat ggaaaaatac
     2941 aggcgattgc ttttgctttt agatataagc aatttgcaaa acattataag gtttatgtgt
     3001 tcagtagaaa aaatcaaatt acagaaaaat attccacgag ggatatggct aaagaccaat
     3061 cagatataat gaaaaaactt ggaattatga aagcagaagt aatgggtgtt tctcaaggtg
     3121 gaatgatagc tcaatatctg gcaattgatt atcctgagtt agttgagaaa cttgtgttgg
     3181 cagttacttc ttcaaagcaa aatgatacta tccaaaatgt tatttgtagt tggattgata
     3241 tggcgaagaa gcagaattac aatggtttaa tgattgacac agcaaacaaa tcctattctg
     3301 aaagatattt aaagaaatac cgattattta ttcctctttt aggaaaagta ggtaaaccaa
     3361 aggattttaa acgatttatt attcaagcga cctcttgcat agaacataat gccttttccg
     3421 aattaaataa aataacatgt ccaaccctaa ttataggagg tgctaatgat aaaattgttg
     3481 gaaataatgc ttcttttcat ttagcagaaa aaattaaaaa aagcgaaata tttatctatg
     3541 agggattggg acacgctaca tatgaagaag cacaggattt caatgaaaga gttttagagt
     3601 ttttaaataa gtaaacacct ttgttaaaaa tatacatata aacattggaa atttaatgtt
     3661 aaaaatagaa acatgaaaag atgcttgact ggtattttta ctatgcctaa caaatttttg
     3721 atgcattatc tactataaat acaaatatag cttcaatgtg attatatgtt ggtgtattgg
     3781 taaagcactt atacaaacag aggaattttg taaattcaga ttatacccat atttgttaat
     3841 ttatgaaaat ataatcaa
//
LOCUS       DAEMAE010000274         3855 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEMAE010000274 DAEMAE010000000
VERSION     DAEMAE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.278"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(143..538)
                     /locus_tag="KPH93_003396"
     CDS             complement(143..538)
                     /locus_tag="KPH93_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG1690167.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPNLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            679..1071
                     /locus_tag="KPH93_003397"
     CDS             679..1071
                     /locus_tag="KPH93_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690168.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESNGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKSTCGLSCKECRKIK"
     gene            complement(1172..1588)
                     /locus_tag="KPH93_003398"
     CDS             complement(1172..1588)
                     /locus_tag="KPH93_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG1690169.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVNESNRIEVDLLINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1603..2514)
                     /locus_tag="KPH93_003399"
     CDS             complement(1603..2514)
                     /locus_tag="KPH93_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1690170.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTAD
                     FAIEMYYPTTPEVAYLEHWIVPVPIEQ"
     gene            complement(2694..3716)
                     /gene="splB"
                     /locus_tag="KPH93_003400"
     CDS             complement(2694..3716)
                     /gene="splB"
                     /locus_tag="KPH93_003400"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG1690171.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKNIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLASAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1287 a          663 c          434 g         1471 t
ORIGIN      
        1 ttattgaaat acacatctaa taactaaaaa tttttacata ttcattattt aaaaatcttt
       61 ttattgtatt taaattaaat ctatcaatat ttcagtaata atgttttctt aactttcact
      121 atggatgtcg attcagttat aattaaagct ctacatcaat ctttttacca ctcattgaac
      181 aacaactaac ttttgcctta tcaatagaga atatttcaaa cttaccatct cctacagagt
      241 acatctgacc taaatttggc attatttcaa gtgccttagc ttgtgtttct tttgatgtaa
      301 caaatactgc ttcacctaaa atacgtagag tattaaggtt ttggtctata caacaaagtt
      361 caacctttgg attaaaaata agttgcttaa acatatcctt ttgattagat gtacaaaatg
      421 taagtttacc attgtattcc ataacaaatc ctaaaggacg cacatgtgct tgattgttct
      481 catcaactgt tgctaaataa taaactcctg ccttgttcaa aaattctaat gctttcataa
      541 ttcttacacc ccttttaaat acttgtatta aggattatcc tttgatataa ttttaatata
      601 aaatacagaa aaaacaagta tgcagttttt tctgataaag tataaaaaag atactactat
      661 aactaaggag taattaaaat gagtataaac actgaaaaaa atataactaa aaacgttgaa
      721 gaattaacct gtccaatccg ttatgcatta gacattgtcg gtggtaagtg gaaattacct
      781 attatatgta tgttagcagt tgaaaatcca attagatata gtagcataaa aagaaaatta
      841 gatggcatta caaatactat gctagctcaa tcattaaaag atttagagtc taatggtatt
      901 gttcatcgta aacaatataa tgaaattcct ccaaaagttg aatatacatt aacaagtaaa
      961 ggtaaaagta ttgtgcctat tttacaacaa tttgctaatt ggggtgctac taatatgcaa
     1021 gaaaaaagca catgtggact tagttgcaaa gagtgtagaa agataaagta aatttaaatt
     1081 ttactaaatt attcgtatta tagcctatgt attaatttat ctgattgaat ataaaaacag
     1141 gatgacttat atatccatcc tgttctatat gttaagctct aaatttaatt agttcaagca
     1201 tatcgtttaa taccaataaa ctatctactt gaatcattag ccatttactt ccattaaaaa
     1261 aatttacctc attatatatt tttttgatat aatcacaaca agtattaata agtaaatcaa
     1321 cctctattct attgctttca tttacaataa ccaaagcttt aaaataacct tcttgtggat
     1381 ataaagtaca tagtgattta cctctttttt tgtattttac attccagcct ctttgcattg
     1441 aacatttact gtattcaagc ttaggttcta cattataagt agaaaccaaa gttttattta
     1501 acttgtccca taatggatta ttaataaatt cattaatttg ctcaaatgta ggtttagtat
     1561 cttgaaaaaa tgcatcttgc caattcatat tcatcatcct ttctattgtt caataggaac
     1621 cggaacaatc caatgttcca gatatgctac ttcaggtgta gtggggtaat acatctcaat
     1681 tgcaaaatca gcagttgtta aattatgctt cttcatccat ctttccataa atttatccgc
     1741 tttaaaaaca gctgaattaa ctaactcatt aaagttttct gcctcaaatc cacatactaa
     1801 atattcttta gctggtaact caaaagtact atatccttct actgatgtac ctgactctac
     1861 ctctacacct gccatataca tacaatttcc ttcttttgca tttcccatat aaagagcagc
     1921 aaattcattt ccacctggaa tttgatttgg tatattatgt ctttgacttc caagtttcat
     1981 ccataattcc tctactattg caatacccgt tgtttcacca cccaccaact ggcaaatagg
     2041 tacttctatt tctattccaa caaacgtcct tggattactc aatgtcttac gattgacttc
     2101 aatcactata tcatctgcaa tcagtggaac accttcatca accatagcat aatttaaaat
     2161 taattctggc ttaataaagt gattgagttt tactggattt gctctatatt tttctggagt
     2221 taatccatat gcttttttaa aggaacgtgt aaatgtctca tgactagcaa atccaaaatt
     2281 taaagctaca tcaagtatac gattcttatg ttctgctaag tattcagaag ctcttgcaag
     2341 acgacgaagc tttatatact ccattacagt cttatttacc aatcttttaa atagtctttg
     2401 atagtaaaat ggtgataaat ttgctttatt tgccaattca ttaattgata tttcttccga
     2461 caaatttgct tctattaaat ccaatgtaat ttgaatagac tcccatgcat gcatattata
     2521 actcctcctg atatttttgt tactctcatc ctttatagta tcaaaactag aaaaaatgtt
     2581 cttgatagat agtgcccttt tatattctta tatgataata ctaaatctca tagctttatt
     2641 ataaacataa aatatataaa aataaaatat agcaaatctt taggtaagat ttgctatatt
     2701 atatatttta ttaaactatt tttaaatact ccatttattt ccttaagaaa gaactctttc
     2761 atatcatcta attgttcttt atcatacacg tacttaccat atccaaattg accgtattta
     2821 aactttctat cctcattttc cattggcaat agtgtatctg gataaatctc tagtattcta
     2881 ttttttgctt tagttgtata tctatgagat ataacttcaa atataatttt tttatcaaaa
     2941 tcctttggta aagcttcttt tatacttata atcagatttt tatactcttc tttccaacct
     3001 tcatataaaa acacaggtgc tattatgaaa ccaatctgat agccagcgct agcaagtttt
     3061 acagctgact ctattctttt atttgcagat gctgtataat gctcatgttc atttataatt
     3121 ctaggagtat tgatactaaa tcgtatttct gtatgattat tgtgttcagc atccaataag
     3181 ctatccacat cactgtattt agtaacaaat ctaaatcttc ctttttcctc tcttccaaaa
     3241 aactctattg ttttcttcaa agaattagta tatggctcta ctggaactgg gtctgatgta
     3301 gccgcccctt caaatatggt tacttgtggc tttctatctt caatatattt ttttgtctgt
     3361 tcaagtattt catctacatt cacaaatacc cttataaatg gtttatcacc taattgtgta
     3421 ttcaaataac aatactcaca tcttcccata catccactga caagtggtaa ttgataatta
     3481 gcagaaggtt tacatgtttg aaacttcaga ctttttctaa ttccaactac catagtctgc
     3541 ttcccacttc tatattgttc atataaattt tcacctggaa tatgttcttt aattctattt
     3601 gatgatatat taattatctc tacatcttta ttatctctaa atttattgta tatattttta
     3661 cccacatcag tgtctaaagt tcctttttcg aatattattc ttttgggatt aaacatttat
     3721 ttcacctcta cttaaagttt ttaaatcgaa gacttttata aaatattact taattaatct
     3781 tccctttttt aaactccttt atttattaga aagtaaaata aaagcttaag ataagtttat
     3841 tgtatatcta acttc
//
LOCUS       DAEMAE010000275         3780 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEMAE010000275 DAEMAE010000000
VERSION     DAEMAE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.279"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   116..802
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=248..276
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1463..3556)
                     /locus_tag="KPH93_003401"
     CDS             complement(1463..3556)
                     /locus_tag="KPH93_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1690172.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRAFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKAIKINEFNEISNTKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWEKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYDQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKMIGVEVLLRWTNDKYKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDNYPFFKVSVNVSAVQILEDTFIKDIVKIIDDENFPYQNL
                     VLELTESHTVQNLSILQSKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1358 a          577 c          356 g         1489 t
ORIGIN      
        1 taaaaaggta ataggatttc tatactacct tttggttttt ataatatagt gtaattttat
       61 tatatttttg tatgctaata taactagata atcataagat aacacattaa aagaaaatta
      121 tattctgtat agttagtata aaacgtaagt aatataaatt tatgtaaagc aacaaaagat
      181 ggatttaaat tccatatagt taatataaaa caaattttgt atccttgaca ttgacatcta
      241 atatataatt tacattccat atagttaata taaaacgcta ctacgtcaac tatttcaaga
      301 gtttcattta aaatttacat tccatatagt taatataaaa ctattcctcg acaccaggga
      361 aattaaaccc ttattttatt tacattccat atagttaata taaaacttaa agttatagat
      421 gaagaatttt tatgtaataa catatttaca ttccatatag ttaatataaa acgcatttaa
      481 agagatttta tctaaatatg aaatggataa tttacattcc atatagttaa tataaaaccc
      541 atttccatat cactgtcttt atacttattt ccaaatttac attccatata gttaatataa
      601 aacaatttta gcaaggaaaa agtgattgaa aagcgtaaga gatttacatt ccatatagtt
      661 aatataaaac tgctgtttgg gatagcaaag aaattaatga ggatgtaatt tacattccat
      721 atagttaata taaaactaga aaaatacttt gtaataagtc gctactatga caaatttaca
      781 ttccatatag ttaatataaa accccaaaat aaacttagta ttttcaatac ttacacgtat
      841 acaactctct taaatttgca gtgaacgagc aatagtgcaa ttgataacgc ttatcatgca
      901 ctctcaacac cttatatttc aactgttacg ccaaattttt tcacaaatat cgctcactgc
      961 aaaatttcta taattttatt atatcataaa tatattactt ttgaatattg ataccaattt
     1021 ataccataat aaaaacaaga aaaaacgtaa tctatctaca ttagatttaa attctataac
     1081 ttaaagtttt ttacataata tttatgcttg tttaatcaat ttaatatatc attcttgtat
     1141 attgctttta atggtctttt ttcttaaata aatatctatc tgaacaagca tagtttaatt
     1201 aaaaactttt gtattcactt aaaactgtat ctacctctta ttattttatt tatgatatat
     1261 tatttttaat ctaaaaatag atatctattt ttattatggt tatccctttt tagaatactt
     1321 taatcatata ctattcctaa tttaacatcc attgaaatgc tattttctta tattacaatt
     1381 tataattgat atatcttcaa tataataatt taatgtattt ttgattttaa agctttttag
     1441 taatattatt gataaattag aattaattat ctagctttaa aaggtcaaag atttctgttg
     1501 ctgtctgcgg ttttccaaat aaatatccct gtatgtaatc tggctttatt tgtttaacta
     1561 aatcatactc ttcttgtgtt tctacacctt ctaaacatac ttttattcct acatcatgac
     1621 aaattgcaac aataaaatgt ataaatgtag catcaaactt actctttaat atatctttaa
     1681 caaatactct atcaattttc actatatcta ttggagaaaa ttttaaaact tctaaagacg
     1741 aatatccagt accaaaatca tccatagcaa tataaatgcc taagtcttgt aaagccttaa
     1801 atttagactg taaaatactc agattttgta ctgtatgact ttctgtcaat tctaaaacaa
     1861 gattttgata aggaaaattt tcatcatcta taatttttac tatatcttta ataaatgtat
     1921 cttctaatat ttgaacagct gaaacgttta cactcacttt aaaaaaagga taattatcaa
     1981 tccatttcct aaaagtacgt aatgccattc gaagcaccca agcccccaca atatttatca
     2041 tatcattttc ttctaatatt ggaataaatt ccaaaggaga aattgcttta tatttgtcat
     2101 tagtccacct taaaagaact tctacaccta tcattttatg actttgtgta tctacttgtg
     2161 gctggaatcg aagactaaat cccctaaaat catcattaat tgatgccttt aaatcccgca
     2221 tcatttctaa tgaatacatt ttatttttta atatttcttg tgagaaaaaa acaagcctgt
     2281 ttttaccgtg ctctttcgca tattgtaaag agtaactagc gcatttataa agttctttta
     2341 ctgtatctcc atgctttgga tatattacac agccagcaga aattgttata tttaatccat
     2401 attttctcca tagttgaaga tgtataatca tattctgtat ttgatcatat aaagttagaa
     2461 tttcattttc ttcaacatta tcaacaagaa tgcccatttc gtctccatca agtttataaa
     2521 gttcagcatt atctggtaaa atagattgta tagactgtgc tagtgttttt attatattat
     2581 ctccaaaatc cctatcatac atctcattaa catttttaaa atcgtcaata tttaataata
     2641 ctatgcataa tttttcaatc ataggatttg aaattttatc ttcaaataca ctcataaatt
     2701 catgataatt cagtaactgt gtcaatgaat caatcttatt ttgtttacct aattgcgtca
     2761 ttattcctgc aaaaatactt ggttctccaa attcatcacg cattagttgc cctctacatc
     2821 ttaaccatat atattctcca ctacgatttt tagccctaaa ttccactgta tgataatcca
     2881 tctggttttt acctatttca gtgtttgact tataaaaacg attccagtct tctggatgta
     2941 caattttttc ccagtaaata agcggatttt caacaatttc tcctggtaag tcaaataatt
     3001 caacttgtga tggtgaatat cgaaatactc ctgttttcat attacaaatg tatatatatt
     3061 catctgtacc tctaatcaaa gcatcataaa gcaatgtaga attataatca aaattgtctc
     3121 ttgaaaaatt aaaaactcct ttttcttctt tagtatttga tatttcatta aattcattaa
     3181 tcttaattgc tttatttaag attgttgagt ttgcaatatc tattgattga tgcatatgaa
     3241 caactgtgcc atcttgccat gtaattaaac tatcataatt ttcaaaagca cgatttaact
     3301 tattacattt ttcataccac tttattaata ctcctttttt atcattcttt aataattcta
     3361 aaactttaca aaaactacaa gtagaatttt gctcaggata taatacttgc cagcaaactt
     3421 ttccttctgg gtctaaaata ttatattctt ctttcatttt cttgttcata aaaataattt
     3481 cattagtatg aatatttgta acatatatat taatctgtag ttgatttaat ataacttcaa
     3541 aattacgttt gttcatatac ttatctcctc catagttact gctctttata acaacactgt
     3601 taaaattatt ctttttagaa gatttactta taatttatac atatatgcta aatactttaa
     3661 tttaattaga aaatttatta cttcatattt taaattataa ttgataaaaa aaaatacagc
     3721 aatatagtat taccaattta aaaaaaatta atattacata atacatctct ctcttatatt
//
LOCUS       DAEMAE010000276         3714 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEMAE010000276 DAEMAE010000000
VERSION     DAEMAE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.280"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            366..1055
                     /locus_tag="KPH93_003402"
     CDS             366..1055
                     /locus_tag="KPH93_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1690173.1"
                     /translation="MDILLVEDDKNIRQMVYDFLISENYSVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRSSQDIVIRYKDLIINISKYVVSKNGVDLNLTHTEF
                     EIVRLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGTEKNNYIKT
                     LWGIGYKMEEE"
     gene            1055..1972
                     /locus_tag="KPH93_003403"
     CDS             1055..1972
                     /locus_tag="KPH93_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1690174.1"
                     /translation="MELFLVVISIVLFSLYLKERNKSKKMESDYKYINSRLRDITNDN
                     ESNYILVPSDISIVKETAVDINKLLEKFYSRQIDYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSTGRFLGISLYEKEKHIFIDIEDKGSGISETEKENIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNIKTVFTVKLKS"
     gene            2045..2971
                     /locus_tag="KPH93_003404"
     CDS             2045..2971
                     /locus_tag="KPH93_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690175.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKSNSGEIVMFGKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2968..3648
                     /locus_tag="KPH93_003405"
     CDS             2968..3648
                     /locus_tag="KPH93_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690176.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGILLCMFFVFTGLNDKATSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILAKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WAFVVGMMRKSIPVTLVSSIIFIHLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVAEID"
BASE COUNT         1404 a          405 c          630 g         1275 t
ORIGIN      
        1 atatttgata attgcataca agataatatg caaattatga tgtctaaatt ccttagaatc
       61 ttgacaataa atttgagata atagataaag aatttttttg aaatttaatg attcagagta
      121 ataatgctaa tagtattttg tatctaattt aatcattcac atctatggga tttacatatt
      181 ttagataaag ataagagagt atactatatt ttaattattt tttcagtaaa gcttattgaa
      241 aagaacttta acttagaaga gtatcgccaa attgcagata caatatcata agcagttatt
      301 tattgacaac tctttttaat aaatatatca tttgaagtat attgacaaaa aggagttgaa
      361 taataatgga catcttattg gttgaagatg ataagaatat tcgacaaatg gtatacgatt
      421 ttctgattag tgagaattac tctgttaaaa ctgccgttga cggaaataca gctattgaaa
      481 tatttaaaaa ttattcattt gatattatct tactggactt gatgttacca aagtctagtg
      541 gttttgaagt attaaagtat atacgaagtg tgagtgttgt tcctgttatt attactactg
      601 cgaaagatag tgacacagat aaaatgctag ggcttaatct tggtgcagat gattatatta
      661 caaagccttt ttcaatcgta gaattgcttg caagaattaa agctaatatt agacgtgcaa
      721 cagtgtatag cagttctagg agttctcaag atattgttat tcggtataaa gatttgatta
      781 taaacatatc aaaatatgta gtttcaaaga atggagtgga cttaaactta acacatacag
      841 aatttgaaat tgttagatta ctagctacca atcaaggacg tgctttttca aaggaacaat
      901 tatacaacac tatttggaaa gagccttatt atggaaacga aaatgtgcta aatactcata
      961 ttaacaggtt aagaaataaa ctgtctgatg gtacggaaaa aaataattat attaaaactc
     1021 tttggggaat tggctacaaa atggaggaag aataatggag ttatttttag ttgtaatttc
     1081 gattgtacta ttttctctct acttaaaaga acgaaataag tcaaagaaaa tggaaagtga
     1141 ttataagtat atcaattcaa gacttcgtga cataacaaat gataatgaaa gtaattatat
     1201 tttagtgcca tctgatattt cgattgtaaa agaaacagca gtagatataa ataaacttct
     1261 tgaaaagttt tacagcaggc aaattgatta taatagaagt gaaaaagcga tattgcaaat
     1321 atttacaaac atatcacacg atttacgaac tcccatcaca gtattaaagg gttatattga
     1381 gatgttatat cttcaaagtc aaaaagaaga actatcttct tcaatgagag caactgtaga
     1441 aaaaatgcaa acaaattcaa atgagcttgt taattcaata aataatctgt ttaatatggc
     1501 aaaaatccaa tctggagata tgatattaaa tcttcaaaaa tccaatctaa cacaattatg
     1561 tcatgagatt attttagagt tttatgactt gctagataaa gaaaacttta atgttgaagt
     1621 taatattgaa gatagaccta tatatgcaaa tgttgataca gaagcattac aacgtatact
     1681 caaaaattta attgataatg ctataaaata tggttcaact ggtagatttt tagggatttc
     1741 actatatgaa aaggaaaaac atatttttat agatattgag gataaaggtt caggtatttc
     1801 tgaaactgaa aaagagaata ttttcacgag agcctatact gctgacagaa agaaaggcaa
     1861 tggtttagga ctagctattt ctcaagggct tgcaaactct atgggaggtt caatttatgt
     1921 aaatagtaga cctaatataa aaacagtttt tacagtaaaa ttgaaaagtt agaaaaaagt
     1981 tagatttaaa agagagaaaa gataatttta ttttgtatac tgtttttaga aaggagggag
     2041 tatgatgaac tatatcatag agacccatca attaaagaaa atatataaag ataaagctgt
     2101 tgttaatgat gttaatattc atgtgaaaaa gggagaaatt tacggatttg ttggaccaaa
     2161 cggagcaggt aaaagtacag taatgaaaat gttgctaaat ctggttaagt caaattctgg
     2221 agaaatagtt atgttcggta agaaagtggc agaaactgat tttgaaatat taaaaaagat
     2281 tggtactatt attgaaaatc cttatttcta tgagaatttg actgctaaac agaatcttga
     2341 cttacattgt gaatatatgg gttattataa caaagaacat atatcagagg tattggaatg
     2401 tgtcggattg tcaaagcaat caaacaagaa agtttcaaaa tattctttag gtatgaaaca
     2461 acgtcttgca atagctagag caattcttac taaaccagaa ttattgattc ttgatgaacc
     2521 tataaatgca ctcgacccag aggggataag agaaatgcga gaattattcc ggaagttaaa
     2581 tacagattat ggaataacta tatttatttc tagtcatatt ctttcagagg tagaacaaat
     2641 tgcagacact attggtatta ttcaagatgg taaacttatt aaagaaattt ctatgtcaga
     2701 tatacacaaa tatcaaacag attatgttga agtagatgta gataatgtgg agcttgctgg
     2761 atacttgtta gaaaaagaat ttggaataaa agactttaaa attacttcgg aaagttgtat
     2821 tgagatatat gatttacgaa aagatgtaaa agaaatatcc aaaatattta tcaaaaatca
     2881 aattgggata aatagcattg gacgtaaaca aagttcttta gaagattact tctttcaaac
     2941 tacaggtaca ggaggtaaag aaacactatg atacatttaa tgaaactaga attaaagaaa
     3001 gttgggctaa aaagatattt tttattttca atgctgggga ttttgttatg tatgtttttt
     3061 gtctttactg gattgaatga taaagctact tcaatatatg attatgatgt tacatttagg
     3121 acaatcagtt tagttttctg tttttactat attatattat ttgctgtgtt gaatgtggca
     3181 tatataataa atgaatatac aaataaaaca atcttgattt tattctctta tccacttgac
     3241 cgtaaaaaac ttattttagc aaagttactt ttgattacga ctcttatgat atcatcaatg
     3301 atagttggat atatctgttg ttttacattc gttgttgtgt tggataagta ttttaattta
     3361 gttattggag attttcatat ttcaatttta tcttattgga taccaggagc aattaaaagt
     3421 attattgtat tctgctcatt aggtatatgg gcattcgttg ttgggatgat gagaaaatca
     3481 atacctgtta cccttgtcag ttcaattatt tttatccatt taagacaatt tattttagct
     3541 ggtacaaata caactcaaga cagtttaatt tctgttttta ttatagttgg tataactgtg
     3601 attggtatat attatatact aaatcataaa gttgctgaaa ttgactagat actattgata
     3661 ggaaggtagt tattaattgt atcttcttgg gataataatg atttgctatt atat
//
LOCUS       DAEMAE010000277         3713 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEMAE010000277 DAEMAE010000000
VERSION     DAEMAE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.281"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(147..569)
                     /locus_tag="KPH93_003406"
     CDS             complement(147..569)
                     /locus_tag="KPH93_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG1690177.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(590..1426)
                     /locus_tag="KPH93_003407"
     CDS             complement(590..1426)
                     /locus_tag="KPH93_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1690178.1"
                     /translation="MKQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            1723..2319
                     /locus_tag="KPH93_003408"
     CDS             1723..2319
                     /locus_tag="KPH93_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG1690179.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILRIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYVY
                     KFESMSYIILLIALFTGIYRIILTPIYAVLLKIVYLFV"
     gene            2349..3068
                     /locus_tag="KPH93_003409"
     CDS             2349..3068
                     /locus_tag="KPH93_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG1690180.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIESIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIKKSSEC"
     gene            3070..3609
                     /gene="scpB"
                     /locus_tag="KPH93_003410"
     CDS             3070..3609
                     /gene="scpB"
                     /locus_tag="KPH93_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG1690181.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT         1417 a          425 c          534 g         1337 t
ORIGIN      
        1 taaaaaatac ccttatgtaa ttatttgcca taggggtatt tttattatca tataaaagta
       61 tttcataatt tttatagaca aatataaaag tgtatgtaac ttataatatc atttggttta
      121 atatcatgta catgattatt taatacttat cgaagtaaag gcatactacc agtaagttta
      181 ttcactttct ttttattacc ataaattgct atcccaaaat actgtaactc atcttctgga
      241 acactagcaa tcttttgtgt aaattcttca taaagattgc aactttgtgc tacatctgaa
      301 aaatctacaa cagttaaatc gttaaattcc aaagtatata attttttacg taaatctttt
      361 attatttctt tgtttccttt taaaataggt acaggaatgt caataattcc taagtggcta
      421 ttacctgttt tatcaattac gtctggtcca acagtttctg gggcatgttt tcctagtgta
      481 attcccatta tagctgctgt attggatata attcccattg gtaaattttc gtctattacc
      541 atgacacatt ttgaattttt agtttccatt tttatttctc ctttttacct tatttaaagt
      601 tgtttgaatc atcatttata aatatatttt gatactgctt tggtgtaaga ccaataaaat
      661 tcttaaaaaa gtttgtaaag tgactttggt ctacaaaccc tgtttgtatc gcagcatcta
      721 taggttctac gcctttttct aaaagctttt tagctttgtt tacccgtata ttttctaggt
      781 atctatatgg tgtaatacct tttaatttcg taaaattacg aagtagagag tatttattca
      841 tacctgctat agtgctcaat tcatcaagta ctatatgttc agcataatta ttttctagat
      901 aatcacatac tgcttgaatt tctatatttg tattttccaa gttagattgc aaatttggct
      961 ctgtatgttc ttcaattaat tgttcaatta ggaagaaaaa aagctcttcc tttttaaaat
     1021 ccaattcttc ttccatgatt atatagtgta attcttgtag tagaggtact agttcactgc
     1081 gaaatataac aggttgatta aattttggta aataattttt acctgtaatt tcaaagacag
     1141 tttttttcat tatttctggc ttaatattga tacatctgta atcaagtact ttatcatcta
     1201 tttgttcaca agtatgacta tcaaaagggt taaaaagtag caaatctcca gtacttgtgg
     1261 tatactcttt atttttacaa gataaatatc tttgaccttt ttctataaaa ccaattacat
     1321 aatgttcatg aaaatggttt gggaattttt gcattatgcc tttaaatcta tatgcttcaa
     1381 tttttaactc tctatcaaag catactgtgc gttcctcttg tttcacttag gtaacctcct
     1441 ttcatgttat acaaagtgta acatatattt ttttacactt cttgtatgat attgactaaa
     1501 acataataat gtcaattcat gtcaatataa tattacactt acagtagtgt aattattata
     1561 ttttcacaac gaatatgata taataataaa agttataaaa taaaaagcaa attttaattt
     1621 tatgcctaga agttaaaatt attataatat aaaataattt tgattataag ttatttaatt
     1681 gttataatag tattaaaaat ttaatttagg agatgtaaat taatgttaag tatgaatata
     1741 ggagaaatag tagcaagttt agttggtata gctatggcaa tatcaataca tgagtttggc
     1801 catgcttatt cagctcactt attgggagat gatacagcta aagcttatgg tagaatgaca
     1861 ttaaatcctg taagacatgt tgacccaatc ggtcttttgg ctatgtttat tcttagaata
     1921 gggtgggcaa agccagtacc agtaaatcca aataacttta aaaactataa gataggaaac
     1981 ttgatagttt cacttgcagg cgttttttgt aatgttttga ccgccatact ttgtgtaatt
     2041 ataaataagt atgtgcatat gtatgctata aatacaatag ctgaatatgt gttcttatat
     2101 agtattggat ttgcagcttt taacttactt cctatacctc cacttgatgg atggggagtt
     2161 atatctacat ttgtaccata taaatggaat gaatatgtgt ataaatttga aagtatgagt
     2221 tatataattt tattaattgc tttatttact ggtatatata gaataatatt aacaccaata
     2281 tatgcagtat tactaaaaat agtatattta tttgtttaat ataataaaca gattaaagag
     2341 ggattgatat gaagtataat atacaattac aagtctatga gggaccatta gatttacttt
     2401 atgatttaat aacaaagcat aaaatagaca taaaggatat atctataata gatataacta
     2461 agcagtatct aaattatttg aaaatgctag ataaaatgga tttagagata actagtgaat
     2521 ttataactat ggcatcaaaa cttttagaga taaagtctaa atatctactt tataagcaaa
     2581 aagatgaaga agaagaccca agaatagaac ttatggaaaa gctagaagaa tatagaaagt
     2641 ttaaggtagc atcacaagat ataaaagaaa atataactta tgttaatgag agattttata
     2701 gaaataaaga agagataatt attgatgaca atgtagattt agaagatata tcaatagaat
     2761 caataaaaaa tatacttcca tacatattta aagttaaaac atcacagata gaaaatgcta
     2821 atgatgagaa actagataaa atagtaagga agaagataat atctgtagaa gaaaagatat
     2881 tgtatataag agatattata aaagataaga ttgaagttac ttttacaaat attataaaat
     2941 catatgaaaa tgatgaaata atagctactt ttttatctat attagagctt ataaaagaaa
     3001 aagaaatagt agtagtacaa gatatatttt ttgatgatat tctaataaaa aaaagttcgg
     3061 agtgttaaaa tgaaaagaga agatataaag tatattatag agtctgttat gtttgcatat
     3121 ggggagccga taagtataaa agagttaaac tatataataa ataaagaact ttcatcaaaa
     3181 gaaatagaga ttatgttaaa tttacttatt gaggagtaca gagaacaaaa tagaggtatt
     3241 caaataatca agttagagaa taagtaccaa atgtgtacaa ataaagacta tgcagaatat
     3301 ataaagaaga ttattgaacc aaagaaaaag aaaagtttga gccaagccac attagaaact
     3361 cttactataa ttgcctataa acaaccaata acaaaggtag aaatagagga tataagagga
     3421 gtaaaatgtg ataaagtatt acaaactttg tttgaaaatg aacttataag agaagctggt
     3481 agattgaata agatagggaa accaataatt tataaaacaa cagatgaatt tttaaagttg
     3541 ttgaatatag aaagtcttga agaattgcca cctatagaaa attatcaaga agtggcaaca
     3601 aatgaataaa aatataaaat cacaataatt tagtatgtaa ttttaaattt taaaagattt
     3661 aaaatgctct aagttgttgt gtttttttgt ataaatataa aattataata ctc
//
LOCUS       DAEMAE010000278         3708 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEMAE010000278 DAEMAE010000000
VERSION     DAEMAE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.282"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(181..750)
                     /locus_tag="KPH93_003411"
     CDS             complement(181..750)
                     /locus_tag="KPH93_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG1690182.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(822..1430)
                     /locus_tag="KPH93_003412"
     CDS             complement(822..1430)
                     /locus_tag="KPH93_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1690183.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKIFDESKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1438..3201)
                     /locus_tag="KPH93_003413"
     CDS             complement(1438..3201)
                     /locus_tag="KPH93_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG1690184.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VKNVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYLTEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKSYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3244..3705)
                     /locus_tag="KPH93_003414"
     CDS             complement(3244..3705)
                     /locus_tag="KPH93_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690185.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMAAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1206 a          665 c          338 g         1499 t
ORIGIN      
        1 tttatatata taattttacc atattacatc atatttttgt aagtttttat tacatttttt
       61 ataatacccc tttattttat aaattaatct aatcgattaa atttatccta tagaaattta
      121 ttacatttaa aaatatgcta gcaaataaaa tatctactag cataaagtct atttataata
      181 ttaatcaact attaattcta ctaaatctcc atttttaacg cctgctgcat ttccctcttc
      241 catatcaaca tgcatttcta gattaaagct atcactagct cttactaata cattttcaaa
      301 tactaatgct cttggtccag atgttttaac ttttactatt tgtttatcag ttacaccaaa
      361 tttagcagca tcttcattgc tcatatgtat atgtctagaa gcaactataa ctccttcttt
      421 catttcaact tctccagcag gtcctactaa tttaacacct ggagttccag ctatatcacc
      481 agattctttt attggtgctt ttactcctaa tgggaaacca tctgccaaag atatttcaac
      541 ttgagtatga gctctagctg gtcctagtac tctaactcct tttaaagttc cttttggtcc
      601 tactaaatcc actttttctt cacaggcata ttgccctggt tgagataatg gtttaaattt
      661 agttaactca tgacctgccc caaataatac gtctatatca gcttgactta aatgaacatg
      721 tttattagat aatgctattg gtaatttcat tgaaattccc tccttttaat tatttaaatt
      781 attgcaatac aattatacat aatattacct acaaaatcaa attatattga atcaatatat
      841 atctttaaat ttttatatat tatatcttta aattcttgat tatctatata agatacatca
      901 tctgtattgt ttaaatatat gttgtgtaaa agtttagtat ttatactctt aaagaaaaaa
      961 tccataacaa tctgtccacc tttaaactgt gaatcatatg atttactccc ccccactgca
     1021 atatacattc ctatccgttt cttatttcta tcaattgaag gtttatttaa tacatattta
     1081 cttgcatata tagcttgagt tctatcaaca acagtcttta atttagctga tattgaatca
     1141 aaatgtacag gacttattac tatagttcct tttgactcat caaatatttt atataatggc
     1201 ttcatatcat cattaaactt acaataccct gttttttcac aaaatccaca tgctgtgcaa
     1261 tattcaatat ccatattgta gatattaaat attttacaac ttaatccaca ctcttctagc
     1321 atagctttta cttcatttgc tataaaactg caatttttat taattcttgg acttccattt
     1381 attattataa tctctggact tttaagtcct tttttatcgt ttgaatccat aatcaatcta
     1441 cctatccttt aacataacta ttaaaatatc atcatcataa ttatcttttg atgagaagtt
     1501 ctttaaatcc aattttagat tttctactat taattgttga tttaatctaa agttttcttt
     1561 tagaaagttt tctaatcggt ttataccata ctcttctttt gatgaatttt ttatctctaa
     1621 aattccatca gtgtacatac acaccattgc ataatcttcc attgaaagag tattattttc
     1681 ataagtagca tcttccatta ttccaatagg tatgcccttt ttacaattta aattattttc
     1741 tattgtccca tctttcttta caactacagg actataatga cctgcattag acacagtaat
     1801 catattttct ttagtatcta taatacctat caaacaagtt gtaaatactc ccatcttgtc
     1861 aaattcatca tataacatcc tatttaaatt tgtcattatt tctcttggcg tattgtattg
     1921 attgcataat actttaaaag caccttttat cattgcaaca acataatttg ataccattcc
     1981 atgtcccatt acatcagcaa caatataaac tatatgttcg tcatccactt ttgtagcata
     2041 ataaaaatcc ccacctacta cccttgctgg tttatggtaa aaataaagtt ctttctcatc
     2101 attacaatcc attttacctt tatccataat aagctgttgt tgtttattta atatgttcaa
     2161 ctcattttca agaactttgt gttttacttc cttttcatta tgttcatata attgcatccc
     2221 aacagaaact tgtttggaca atatactaag aatatttaaa tcatcactag tatagctttt
     2281 actatttgca caaactatac atcctatagt ctcattttta tcctgaagtt tgtaatatat
     2341 ataactttcc aaatttattc ccttttttga gcatggtttt aaattattta ggtacccccc
     2401 caatgactct ttctttaaaa acatatcaat cataggatat aaatttgttt tctttttttg
     2461 ctcaacgtta taacaactaa ctatagtatc cttattatga tgactattta taattattaa
     2521 agcttcttta ctattagtaa tattgcacgc ttgtttactt aaattttcta aaaagccatc
     2581 aatactctta ttaaggtata gttttttagt agcttcattt atggcactta ttgagcctct
     2641 tagtttatca attaaaattt gtacattatt atttttatta gttatttcta ttgataaact
     2701 taagagtgat gcaacagatg atataaaatc tatatcttgt tctgttagat aatcatcctc
     2761 ttttaaaaaa caagtcataa atccaacaac tacgttattt attataaaag gaaaaactat
     2821 tctcccaata taaccttcag cttttgctaa atctcgttca ccttctgctc tagaatcttc
     2881 aaatacattt ttcacatata ttatcttttt ttcatttaca gcctcatgta tatactctgg
     2941 atacgtgtca aaatcaattt ttgcaccttt aagactattt attggaaaaa tttgaggtac
     3001 atattctaag gtttgcgaac atactaagta ggcatattta gaatcatttt tataaaatag
     3061 attcacacat gcctttgtag ggtgaacaac ttctaacatt ttttctatta tgtcatcttt
     3121 tatattaaaa aaatctgtag attcagtcac catagcggat atctccacta aattcttcat
     3181 tttataactg ccattagtca tactcaattt ctccccctat atattaaact atttactagt
     3241 attttaacat acattcttta tagtaaaaac aaaaacaacg acaaaactag caactaatat
     3301 ccagttaaat cctgtcaata atcctataag aagtaatgta attaatgcat ttagacctgt
     3361 tctccctttt tcatccataa aagttatttt agagatgtcg atattatttt tcacattatt
     3421 tatatgctct tttacttgtt cttccatatt tcttttatat agtatataaa ctacaccaac
     3481 taaaaacact cctataccta gaaccatgtt taaattaaaa gctgccataa gcaatattga
     3541 accaaaagct aactttaaaa gcttaaaaaa tacgctatct atctttagag ttatatttct
     3601 gatttcttta cattcatctc ttaaagaata tccacttgaa tctaagttat taatctttgt
     3661 ttttacaatt tcagaataaa aaagctttat tataccattt aacatttt
//
LOCUS       DAEMAE010000279         3646 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEMAE010000279 DAEMAE010000000
VERSION     DAEMAE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.283"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..1849
                     /locus_tag="KPH93_003415"
     CDS             230..1849
                     /locus_tag="KPH93_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG1690186.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2223..2417
                     /locus_tag="KPH93_003416"
     CDS             2223..2417
                     /locus_tag="KPH93_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1690187.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2977..3300)
                     /locus_tag="KPH93_003417"
     CDS             complement(2977..3300)
                     /locus_tag="KPH93_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690188.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYVI"
     gene            complement(3345..3599)
                     /locus_tag="KPH93_003418"
     CDS             complement(3345..3599)
                     /locus_tag="KPH93_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690189.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
BASE COUNT         1341 a          466 c          456 g         1383 t
ORIGIN      
        1 ataaaatact aaaaatctaa gttcaagtat ttaaattgct atatatcata aattttctaa
       61 aattaacaac aacttaaaaa tttaacattc tcttaacgta tacttaacaa aacattaata
      121 tttttattga caaaatttgt tattctatat tatggaaaca ataaatttta gttaataaag
      181 tttataattc agataaaaaa attattaatt tcttaaggga ggaaatatcg tgaatatagt
      241 aattagcctt atcggtggac ttggactttt tctttacgga atgagtttaa tgggagaagg
      301 tttacaaaaa tcagcaggag acaaattaaa aaaaattatt gaactcttaa caagtaatgt
      361 tgttatgggt gtactggtag gtacagtcgt tacaggtatt atacaaagtt caagtgctac
      421 tactgttatg gttgtaggat ttgtaaatgc aggtataatg aatctaagtc aagctattgg
      481 tgtaataatg ggtgcaaata taggtactac agttactgct caattagttt catttaatct
      541 tgaaggaatt gcaccaattg ctttaggtat aggtatttta ttctacttat ttacatcaaa
      601 tcaaaaaaca aagcatttat ctgaaatact tataggtttt ggtatattat ttacaggaat
      661 ggagtttatg aaagatgcag tagctccact tgctgaatat aaagcattca ctgatgcact
      721 tttatatttt agtaaaaatc ctgtattagg tatattggca ggatttgcca taactggtat
      781 tgttcaaagt tcaagtgcat ctatgggtat gttaatagca ctagcttctc aaggaatact
      841 tcctctatca tctgctttac ctattcttta tggagataat ataggtactt gtgttacttc
      901 acttttatca agtgttggtg caagtagaaa tgctcgacgt gcagctgtaa tgcatttaag
      961 ttttaatgta attggtacaa taatatttat gcttgtttta aataaaccta tatcagccat
     1021 agttactcat tttgacccaa ctgatacagc tagacaaata gcaaatgcac ataccttatt
     1081 taatcttaca aatgttataa tattattacc attttctaaa tacatagtta aattagcaaa
     1141 tagacttata cctattaaag aaactgaatc agaaatagta aataatacta agtacttaga
     1201 tgaaaggatg tttagcactc catctatagc acttggtaat actgtacaag aagttgtaag
     1261 aatgggtcat aaagctacaa actctttgga acattcaatt gcaggatttt taaataagag
     1321 caatgaagat attaataaag cttttgagtc agaaaaagtt gtcaataagt tacagaaaga
     1381 tatattgaac tatttgttaa aactttctaa agaacctctt agagatgatg aacgctttag
     1441 aacagattta ctttttaata ctgtaaatga cattgaaaga gtctctgacc atgcagaaaa
     1501 tatagctgaa ttggccatga gtgttaagga aatgaatatt agtttttctg atagtgcgat
     1561 aagagaaata tacgaaatat acaataagac aattactaat tttaaggatg ccttaatagt
     1621 tttagatgtt aaagattttg agttagcaaa taaagtttta gaagttgaaa atgaagtaaa
     1681 ttatttagaa aaaactttta gaaattcaca tatgattaga ctaaataatg gaagttgtac
     1741 aattgatgct ggtgtacttt atcttgactt acttactaat ttagaaagaa tttctgacca
     1801 ctcaacaaat atagtaaaac aagtattaaa acttaaacaa aaaatatagt gattattttt
     1861 atatataatc ttaatcaatg agaaacaaga agctatagat aatgcttata tcatttatag
     1921 cttcttattt ttaatgtaat actcaaattt ttatagtatt ctcttattat taaacagttt
     1981 ctgcctagaa tattccatat atatatgact tctttcataa aacttgaact gcccataact
     2041 aaagtcacaa gtatacatag ctatttatta atcaattata accaatattt attttattta
     2101 tcttcatttt aattcttcat tcaataatgt aataagatta ttttttattt ggatactatt
     2161 atatcatcta aatatgttat tatatatttg gatattttca tattaatagg agggattcta
     2221 caatgataaa ttatatggta gaaattatat atagcgaaaa aataagtgaa gtagaaaaca
     2281 tgttgaaaat tgaaaatata caatatgaat ttacttctcg tgatggcttt atattttttg
     2341 gtttttatga gatgacagac gcatatagat ttgagacttt atgttcagat gaaaaaattc
     2401 gatttaaaag aatataatag ataaaaaaag tacatatttc taaactattt gtttttcaaa
     2461 tttaggttgt gttttaatgt agtgttgata ttataaacaa ttttaataat aaaaaagtag
     2521 ggacattata ttaatatgct cctacttttt actcatactt attaatatta tttatcaaaa
     2581 ttaccctaca aactggaatt tatcattctt tcataatttt aaactatcat atttaagata
     2641 tataaaattc tacatgattt ttgtataaac ataatatcaa tcattatcca atttaaaaat
     2701 attttttagt aatttccatt tcattttctt ttaacatatt aattaaactt ttatatgtaa
     2761 atggtgtatt tacatattat ttaatatcca acaattatat aaagaaatgt gctctttgcc
     2821 ttataaagaa ttataaagcc aattcgtttt attatattat ttttctttat accttaaaaa
     2881 ctttaccttc aaattcttat ttatctatat attataaaaa tgttcttaac ctcttttacg
     2941 agattttctt gacatattat cagttagata aaataattaa ataacataat cagcaagtat
     3001 gttttttact cttttaagta agtttccacc tgcaccccaa tcaattttta aaattccaat
     3061 tgcactgcct gttgaagtag ctttaataac tgtttttcct gtggtatttt cataaactac
     3121 tattaatcct gcatcactat tatttcttgc ataatacttc tcaaaagttg taattagtat
     3181 aaatttatca tctcctaaat tataagaatc actaaaaact aaatctgcat caagattttc
     3241 ttttatcatt ttctgtgctt ctaagggtgt aatttttaca gtaaatattt ctgttgccat
     3301 tttttatctc ctcataagaa tttattttag acaaaacata atctctagta ttttctgata
     3361 tatttatcaa acaatatccc tattcctcct aacagagcta atatagaacc aaaatttcta
     3421 tattgagaaa tagattggtc tagataaata ttatattggt cagtatccat gattccacca
     3481 ttttgttgaa taatatgctc tgcactaaat tcacctaaac ctatacagca gaataatact
     3541 actactccaa ttacaaaagc aaatatacat attgttaatt taaaatcctt attttccata
     3601 gttatccctc tcataagcta aatttatttg attattttta ctagat
//
LOCUS       DAEMAE010000280         3644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEMAE010000280 DAEMAE010000000
VERSION     DAEMAE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.284"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..805
                     /locus_tag="KPH93_003419"
     CDS             <1..805
                     /locus_tag="KPH93_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690190.1"
                     /translation="RESDFKKLRQYQNNNYEELTSKESVYYVSDSYKKIFFKDFEIEK
                     INLHLKDTNEYDINLFKVQKSILEPEINSQATFDLIVVKDDVFNQLKLHENSRLIRLI
                     DIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSIESYNNLIKLSGLMLFIGIFLSVV
                     FLLCTGSIILFKQLSNIYDDKERYIMLTKLGANNKDIEKIISKQLKVIFLMPLVVGTV
                     HNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            1030..2643
                     /gene="ggt"
                     /locus_tag="KPH93_003420"
     CDS             1030..2643
                     /gene="ggt"
                     /locus_tag="KPH93_003420"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG1690191.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKGGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2776..3627)
                     /locus_tag="KPH93_003421"
     CDS             complement(2776..3627)
                     /locus_tag="KPH93_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG1690192.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSNFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1408 a          463 c          579 g         1194 t
ORIGIN      
        1 tagagaaagt gattttaaaa aattaagaca atatcagaat aacaattatg aagaattaac
       61 ttcaaaggaa tctgtgtact atgtttctga ttcatacaaa aaaatatttt ttaaagactt
      121 tgagattgaa aaaataaact tgcatcttaa agatacaaat gagtatgata taaatctgtt
      181 taaagtacaa aaaagcatct tggagccaga aataaattca caagctacgt ttgatttaat
      241 tgtagtaaaa gatgacgtat ttaatcagtt aaaattacat gaaaattcta gattaataag
      301 attaattgat ataaaaaatg aaaaaaaaga actggatttg actttaaaat taaaaaatat
      361 agtaagtgaa aatatgaaat ttacttatcc ttttaatttt acatcaagta ttgaaagtta
      421 taataacttg ataaaattaa gtggtctaat gctatttata ggcatatttt tgtctgtagt
      481 atttctttta tgtacaggca gtataatctt atttaaacaa ttgtcaaata tatatgatga
      541 taaggaaaga tatattatgt taacaaaact tggagcaaat aataaagata ttgagaaaat
      601 aatatcaaaa cagttaaaag ttatattttt aatgccatta gttgtaggta cagttcataa
      661 tttatttgct atgtctatag cacaaaagtt tataccaagg tcattactcg taccaattat
      721 cataactcta gtcatttatt ttataggata ttttatatat tattttttaa ctttaaaata
      781 tgctctaaac atgataaaag aataaattat ttttaagtta aaagaattag atatgtgttt
      841 tgaataaaat aacttattta agtgcagata gaaaatacta gaattgagtt taagatatct
      901 atttaaagat aaaattaaag ttaggaaaaa gtctatattt tataccattt tatatcagtt
      961 acattacatg atgttatata ataaatctaa gcagaatatt aaataaaaaa tgacgaggag
     1021 ctgaaattta tgaagtttaa tgcctaccaa cataaatact catcaataag aaatgtcgtg
     1081 tatgctaaaa atggagcagt tgcaacatca acaccattag catctcaagc tggacttgaa
     1141 atactaaaaa agggtggaaa tgctgttgat gcagcagttg caacagccgc cacattagca
     1201 gtagtagaac ctacaagcaa tgggattgga ggagatgcat atgcccttgt ttggatagag
     1261 gaagaaaaac gattatatgg tttaaattct agtggctttg ctccagaaaa tatggaatta
     1321 aaaaattata ataatatgaa agaaatgcca aaatatgggt ttggagcagt aactgtacct
     1381 gggatacccg cagcttggtc agaattaaat aaaaaatatg gtaaacttag cttaatggaa
     1441 tgtttatctc cagcgataaa ctatgcaaga gagggctacg tagtatctcc taatgtagct
     1501 aaagtatgga aaaaatcata tgaactatat gaaaaagaac ttattggaga agagttcaaa
     1561 ccatggtttg atacattttc taaagatggt aaagctccag aggctggaga tatatttata
     1621 tgtgaagaac aggcaagtac attagaagag atagcaaata ctcaggctga aagtttttat
     1681 agaggaagac ttgctgataa aatagatgaa tattctaaaa aatttaatgg agctataaga
     1741 aagagtgact tagagtgttt ttatccaaca tgggtagaac ctataagtac agaatataag
     1801 ggatataaaa tatttgaaat cccaccaaat gggcatggaa taaccgtttt aatggcactt
     1861 aatatattaa aagaactgga attagaagga aatatagaaa atgtagaaga catacataaa
     1921 attatagaga gtttaaaatt agcttttgca gattctaaaa catatgtaac tgatatagaa
     1981 catatgaagg taaagattca ggaattacta agccaagagt atgctaaaaa aagaagttta
     2041 ttaattgaca ataaagaggc tttgtatcca actgcaggag agccatattg tggtggtaca
     2101 gtataccttt gtactgcaga taagggtgga aatatggttt catatataca gagcaattac
     2161 ataaattttg gttcaggaat tgtgatacca agaactggga ttgctttaca tagtagaggt
     2221 aataatttta atttagaccc taaacaccac aatgtagtaa aaccatttaa aaaaccttat
     2281 catactataa tacctggatt tttaggtaaa gaagataaag caataggacc atttggtgta
     2341 atgggagctt ttatgcaacc acaaggacat attcaagtgt taacaaatat gattgatttt
     2401 ggattaaatc cacaagaggc gctagatgct ccaagatggc agtggataaa aggaaaggaa
     2461 atagaagttg aaccagagat gccaaaacat ataatagatt cattaattga aaagggtcat
     2521 gaaataaaag taattcatga tactgtcgac atgggacgag gtcaaataat atttaaaaca
     2581 gaacaggaaa gttatatttg tgggacggag tcaagatgtg atggtcatat tgcagtttat
     2641 taatattatc aactaaaata tatataataa atttaaagag tatatgaaat aataagtaaa
     2701 ggcgaaagtc tacaatgact gacgccttta caaaatttaa gctatcatat gctgatttta
     2761 tatagattat cagccttatt cttgaccagt tttgatttca ttgtcatcat aactaatcca
     2821 gtcactaaag ctaccaatat aaatcttatg aggtatatcc agttctctaa gagcaagact
     2881 gttaacacaa gcagatatcc ctgaaccaca agataaaatt acttcatcat aattatttaa
     2941 atctttaaag aattttttta aaaattctat atctttaagt gagccatttt caaagttact
     3001 ttttattaaa tccttacaaa aataattttt tgcacttggt atatgaccag cttttgagta
     3061 agcaggttca actaaacctt ggtatctttc attagcacgg caatctatta ttacagtatc
     3121 ttttttgtat aatttagact taacatattc cattggaaca actaaatcat tgtttatatt
     3181 tggcctaatt tccttacctg tacaacttgg tatatttact ttctcttcca attctccacc
     3241 ttcctttacg aaagatgtta ttcccccatc tagtacatag acatttttaa gtcccaaatg
     3301 ttttaattgg aaaaataatc tgcaagctcc atttaaatct ccatcatcat aagttacaat
     3361 tgtagtgtca ttatcaacac ccattttttc taatttttct tttagtatta aagggtcttg
     3421 taaaggattt ttaccaccat gttcttttgt aggagatgat aaatccttgt ctatatctaa
     3481 gataaaactg cctttgatgt gtcctttttt ataagagtca ataccataag ttctattgat
     3541 aagatcaaat ctacaatcta ttacaactag attatcattt ttttctagtt ttgatataag
     3601 ttcttttgct gaaataatgt ttttcaaaga atgtacctcc tata
//
LOCUS       DAEMAE010000281         3619 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEMAE010000281 DAEMAE010000000
VERSION     DAEMAE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.285"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..718
                     /locus_tag="KPH93_003422"
     CDS             59..718
                     /locus_tag="KPH93_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG1690193.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAITGLVIMQTLISFLSLKSRRLSSFLSGKPSVLIDKGKIVY
                     KELKKERVTIDELLEQLRVQGYFNLKDVQYAILETDGNLSVVPASTYNSTPPKAFNHL
                     PIPLILDGRIINKNLDIAEKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            721..1101
                     /locus_tag="KPH93_003423"
     CDS             721..1101
                     /locus_tag="KPH93_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG1690194.1"
                     /translation="MKSATFVILWTILFVLFGFYINNKLYDFTDEYKDNISVLEKSIE
                     NEDWEKAQKEADSISTRWAKEKNHWYKVLNHEYFDEIGLKFNILDKAIYTENKLKSLE
                     EVEGIKTYLGNIVESVKFDVNYIF"
     gene            complement(1190..2353)
                     /locus_tag="KPH93_003424"
     CDS             complement(1190..2353)
                     /locus_tag="KPH93_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1690195.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQRQLLILKRKRLDELIELINQ
                     TLRGEGNINFKEFDMSEYFDVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEQHPKLKELYRKLVEDL
                     NKNPSSTELQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREIQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(2446..3507)
                     /locus_tag="KPH93_003425"
     CDS             complement(2446..3507)
                     /locus_tag="KPH93_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG1690196.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYIKTNKADLKRMVALGDGRTKQMYEFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRQSMNCNYPGLSKENTICTIRMNTLKNKYNIAREAIAIKRDMFLSPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVAEIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDVQKIYC"
BASE COUNT         1247 a          559 c          410 g         1403 t
ORIGIN      
        1 ttttatatag gacatactaa ctaagtgtaa atatttttta aaggagtaat tgatagatat
       61 gacagtagta ttaataagaa gtattatatt atacataaca gtactaattg cacttagagt
      121 catgggaaaa ggtgaaatag ctgaaatgaa ctgttttgat ttagttataa cactattaat
      181 tgcagaagtt gcatctgtcc caatggaaaa taataatata ccaattataa atggtgttgc
      241 agcaataact ggacttgtaa ttatgcaaac tctaatttca tttttatcat taaaaagcag
      301 aagattaagt tcatttttat caggtaaacc ttctgtacta atagataagg gaaaaattgt
      361 atataaagaa ttaaaaaaag agagagtcac tatagatgag ttattggaac aacttagagt
      421 tcaaggttac ttcaatttaa aagatgtaca atatgctatt ttggaaactg atggtaacct
      481 aagtgttgtt ccagcatcaa cttataatag tactcctcca aaagctttta atcatttacc
      541 aattccactt atattggatg gtagaataat aaataaaaat ctagacatag ctgaaaagga
      601 tactaattgg ttgatgggta ttttaaagtc taatcacata gaaactttta aagatgttct
      661 tatttgtgtt ttagatgaaa atgacaaaat atttattcaa aataaaaagg gtgattaata
      721 ttgaaatctg caacatttgt tatattatgg actatcttat ttgtattatt tggattttac
      781 attaataata agctatatga ttttacagat gaatataagg acaatatatc tgttttggaa
      841 aaatctatag aaaatgaaga ctgggaaaaa gctcaaaaag aggccgattc catatctaca
      901 agatgggcta aagaaaaaaa tcattggtat aaagttttaa atcatgaata ctttgatgaa
      961 ataggattaa agttcaatat tctcgataaa gcaatttata ctgaaaacaa attaaaatct
     1021 ttagaagaag ttgaaggtat aaaaacatat ttgggaaata tcgttgaaag tgttaagttt
     1081 gatgtaaact atatatttta ataactttaa ataaggatat agacagaact aatctatatc
     1141 cttatttttg tgcttttata aagcttacac attttttact tccaaatttt cagcaattat
     1201 tattatcaaa ataaatctta aaagcttctc caataaactt agatgcacca tcaccataat
     1261 tcttatcagt tacttttata tagttagaat cttgtaaata aagctctgac atataatcaa
     1321 aatatctttc gccattgtct attttataaa attcattact tcttttcatt tcattgtcta
     1381 tttcttcaac aatactttga atttcccttg aagaacaatc tctactcaaa tcttctacaa
     1441 gtttttcata caatgtattt atctttggtt ttctatctct tagataagtc tttagtactt
     1501 gtccaataaa cttacctgct ccacttccat actttttatc tacttcttct atccacatag
     1561 gatttactag ataattttga accatataat accagttgtc atcaccagta tccatagaga
     1621 aaatttcata atctttttta gatatatctg ttatctcttt tgctatttct tgaagttctg
     1681 ttgaagaagg attcttattt aaatcttcta caagttttct atataattct tttaattttg
     1741 gatgttgttc ttttaaacaa tcatttttat atctatcaaa tttttctcct aaattcaata
     1801 tatcactgct aaaatttgtt tttatagctt gagcaaactt ctcaatagtt ccatactgtt
     1861 ttatagccat tttagcaatt ttttcttcat tggatttaac tctttctata tattcattgt
     1921 atttttctac actaccataa attttaatta tcttattcct atgctctctt ttaaattctt
     1981 ctaaaacatc gaaatactca ctcatatcaa actctttaaa attaatatta ccttctcccc
     2041 ttaaagtttg atttataagt tctataagct catccaacct ttttcgtttt aaaataagta
     2101 attgtctttg attttttaaa gcttcaacct tatcaaaata ttggcttgac attatttcct
     2161 taacctctct taaaggaata tcaatctcct taaaaaataa aatttgttga agagttttaa
     2221 tagattcatc atcataaagt ctatatcctg catctgtaat tttatttggt tttaaaagac
     2281 ctatttcatc ataataatgt aatgccctca cacttattcc agttaaatca gaaacttgtt
     2341 tcactgttct catatccaca cctcaaattt taattttaca gctatcaata cctactaata
     2401 tcttcaagct atattttcat attacactat tacgtaatgt tagggtcaac aatatatttt
     2461 ttgtacatcc aagattaaat atcctgtttt catatgctct aagacctcag agaataataa
     2521 cggaagttgc tctgtatcat cacatgtttg agttaaataa tctattataa tgttggatat
     2581 tttctcaatt ataatagcag ttttatcaaa aatctcagct acttgtataa aagagttgtt
     2641 tttgtattcc tccccaatac tgttgagcat tctactcatt ttatcaaatc cacctttaaa
     2701 ttgaacagta tctggttctt taattccttt gattgcattt ggaattgttg gtactgttcc
     2761 aaaaaacata accatattgg tcaaaatctt atcataatca tctttatcca attcagtgtt
     2821 ccactgtggc aactctttaa taaacttttc aaaaccttta tatcccaaaa atcctttagg
     2881 tggacttaaa aacatatcac gctttatagc aatcgcttct ctagcaatgt tatatttatt
     2941 ctttaaagta ttcattctta ttgtgcaaat agtattttct ttgcttaatc cagggtaatt
     3001 gcaattcatg ctttgacgta atttatcata agaaagatgt aataattctg ttctaccaca
     3061 atcatacaga tatattcctt catcatcata tcctaccatt aatatataat gaaaaggaat
     3121 gtgttcttta tgatatagct ttttataata tggtagataa tacatatcta aagctcctaa
     3181 aataactgga tatccaccat caatttcaga ttttgctttc tttaatgctt gctcatattt
     3241 tttaaactca tgatgtttgt attcaaagcc aacaattggt gctaaaaatt catacatctg
     3301 ttttgtcctt ccatctccca aagctaccat tcttttcaaa tcagctttat ttgttttaat
     3361 ataacaaaac gaaccaaaac ctgatagtaa aaagaaaaaa tcatttggaa ggcattcacc
     3421 tgttttgttt atatataaat cttctatacc attccacata cactcataat catgtgaatg
     3481 atgtttaatt tcaattattt ttctcatata atctatctcc tcactataaa ttttcattag
     3541 attcagtata aaccctctta caatgagaga gtcaataact attgtattta taaaatttat
     3601 attttaggct ttatatttt
//
LOCUS       DAEMAE010000282         3613 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEMAE010000282 DAEMAE010000000
VERSION     DAEMAE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.286"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..1498
                     /locus_tag="KPH93_003426"
     CDS             122..1498
                     /locus_tag="KPH93_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG1690197.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPATIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1798..2469
                     /locus_tag="KPH93_003427"
     CDS             1798..2469
                     /locus_tag="KPH93_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG1690198.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2658..3455
                     /locus_tag="KPH93_003428"
     CDS             2658..3455
                     /locus_tag="KPH93_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG1690199.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNVIDASQDETVDMLKSLDFNIVSSYLNTDNFYDKIDYGSKVALVIG
                     NEANGINEELVSKSNILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1365 a          402 c          672 g         1174 t
ORIGIN      
        1 atcattgtct ttatacaata tttatactat aatagaataa taatttagag gaaagctcta
       61 aaaataaaca aattaagggt cttaacagta gtgattttat gttaaggagg tataaagaaa
      121 gttgaaaaca atagttagaa gacttattct ctacgcagat ggaatgcgtc ctactcaaat
      181 aatggtatct gggtttgctg cgattattgt aatcggagca ttgttgttaa cactcccgat
      241 agcgtcgcag agtggagaga gtataggctt attaaatgct ctatttacgg ctacttcagc
      301 ggtttgtgtt actggtctag ttatggtaga tacagcaact tattggagtt tatttggaca
      361 gatagttata ataacattga tacaaattgg tggtcttgga tttatgaccg tagccacaat
      421 gttttcactt atggcacgaa aaaaaataca actaagagaa agattattaa tacaagagtc
      481 attgaatcaa gctgatttat cgggacttgt aagacttaca agatttgtat taataataac
      541 cataacaata gagggaatag gtgcactagt tttatcaact gtctttattc cacaatttgg
      601 tttgtctaaa gggatatggt atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg
      661 atttgacttg atgggcagtg taagtggccc atttacatcg ttaaattctt atgtaaacaa
      721 ctttacagta tcaatgactg tttgcgcact tatagtgctt ggagggctag gattccctgt
      781 tgtacttgat attgtaagaa aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt
      841 attgttttca actgctacac ttatttttgt gggtgcatta tttatttttc ttatagaatt
      901 taatcaaaaa gctactatgg cggacttgcc tttaaaggga aaagtactat cagcaatatt
      961 tcaatcagta acagcaagaa ctgctggttt taatacgctt gatttagcta ctttacgaga
     1021 aagtagtgta tttgtaatga taattttgat gtttattgga gcatcacctg catcaactgg
     1081 tggaggtata aaaacaacaa cactagctgt tttaatcatt acagttagaa gttttctatc
     1141 aggaaagtca gatatagaag cttttgaaag aagattagct ccagcaacta taaaaaaatc
     1201 tcttggtata tttgtaatca gtatatcagc agttattttt gggacattga ttatttcaat
     1261 aactcagcct aattttacat tagttcaatc agcttttgag gttacatcag cccttgctac
     1321 agttggttca agtttagctg gaactccaaa cttaaatgct ttaggtaaga ttataataat
     1381 aatctttatg tttatgggta gagttggttc tcttacttta tttatggcta tactttctgg
     1441 tggtagaaga aagagtcaac caatcagata tgcagaaggt aaaattatgg ttggttaagt
     1501 taccaaggag tagtataaat ttataatgat ttagagtagc actgtaaatc tgttgataaa
     1561 ataaatgaac ctagatgtaa ttatatgaag aaataaaatc gactaacgaa caaaattaat
     1621 taataaaata aaaaacagtg aaataaaaaa cagtgaaata aaatacaaat aaaaaaggta
     1681 aatcaaaatt aaattaatct atgtatataa aattatatat tgtgtataag ttttgtatgt
     1741 gcaaacatta atttaagaga tactatataa attgtgtatt agggaggaag tttcgagatg
     1801 aagcagtata tagtcattgg gtgtgggaga tttggaagtt cagttgcgtc tactatgcat
     1861 cttttagggc atcaagtaat ggcaatagac aaaaatgaag attcagttca aagtatatct
     1921 gacaaggtaa cccattcact tatagtggat gttactgatg agcaagcgtt aaggtcatta
     1981 ggtttaggta actttgatgt agcagtagtt gcaataggtt ctgatataag ggcatctata
     2041 atggcgactc ttatagccaa agaaatgggt gtagagttga taatatgtaa ggcaaaggat
     2101 gaattacaag ctaaagtgct ttataaaatt ggcgcagata gagttgtatt tccagaaaga
     2161 gatatgggag taagagttgc acacaattta gtttcggata atatattaga ccatattgaa
     2221 cttgacccag agtattcaat tgttgaaatc gtaactccaa atagttgggt tggcaagaca
     2281 cttatagagc ttgaattaag agctagatat gagataactg tacttgctat aaaaacaggt
     2341 aaaaatataa atgttacacc ttctccagat gaggaactta cagctggaag tatcctagtt
     2401 ataatcggtc aaaatactag tataacagcg ataacatctg gaaataaggg gataattaga
     2461 agaagataat ttactattta atatatattt aattgcaatg aaagtagata gtatcatata
     2521 attatgaata gttatatgat actatttttt taatcgaagg tagtatttat ttgtaagatt
     2581 agataaagat gagttaaatt aaaagtgagg aggctatgct aatataaaaa tttataagtt
     2641 attagcattt agataaaatg attacaaata taaacagtaa ggataatgaa aagttaaagt
     2701 atacaagagc actattaaaa tcaaagaata ggaataaaga gtcaaagttt ataatagaag
     2761 gatacagaat agtaatgctt gcacttgaat gtatggcaaa ccttgattat gtatttatca
     2821 atgaggaatt tgaaaataag aaagaacatg taaaactatt agaagatttg gataaaaaaa
     2881 acacaaagat atacaagact actaataaaa actttaaaga attagtggat acagaaaata
     2941 ctcaaggaat aataggtgta gtttcattta agaaaaaaaa attaagtgaa agtataaata
     3001 aaaaagataa atttgtattg attttagata gaatacaaga cccaggaaat atggggacta
     3061 taataaggac tgctgattct gctggggtag atgccataat agcactaaag ggatgtgtcg
     3121 atatatacaa cccaaaagta attaggtcta ctatgggttc tatttttgat atgaatgtaa
     3181 ttgatgcttc acaagatgaa actgtggaca tgcttaaatc attggatttt aatatagttt
     3241 caagttactt aaatacagat aatttttatg acaaaataga ttatggttca aaagtagcat
     3301 tggtgatagg aaacgaagca aatggaataa atgaagaact tgtatcaaag tctaatattt
     3361 tggttaagat acctatatat ggtaaagctg agtcgttaaa tgctgcgata agctctgcta
     3421 tactgatgta tgaaataaaa aaatacttaa tttaatgtat tgtaataaat ataatgtcat
     3481 gttataatct tgaattaaat agtagagtat caaaaatagt caatatatat taaaaaaata
     3541 attaaattaa ttatatatta gatgtatatt atataaaaaa taattaagtt aattacatat
     3601 tagatgtata tca
//
LOCUS       DAEMAE010000283         3606 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEMAE010000283 DAEMAE010000000
VERSION     DAEMAE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.287"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(316..516)
                     /locus_tag="KPH93_003429"
     CDS             complement(316..516)
                     /locus_tag="KPH93_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1690200.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(740..916)
                     /locus_tag="KPH93_003430"
     CDS             complement(740..916)
                     /locus_tag="KPH93_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690201.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFIIEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(970..1059)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1239..1439)
                     /locus_tag="KPH93_003431"
     CDS             complement(1239..1439)
                     /locus_tag="KPH93_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690202.1"
                     /translation="MTKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1441..1791)
                     /locus_tag="KPH93_003432"
     CDS             complement(1441..1791)
                     /locus_tag="KPH93_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG1690203.1"
                     /translation="MIQKKFFMGFCGFLGFLSLKYFSSGNIADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQFAIIELFILWCIAIVSRNVELMCVLLSITYAVT
                     LNVYAIKLYILEEK"
     gene            1952..2140
                     /locus_tag="KPH93_003433"
     CDS             1952..2140
                     /locus_tag="KPH93_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690204.1"
                     /translation="MTKYDFNLKLKVAKFYLNREGGDVLIVKQYGVYGHSQVERWVNI
                     LGESALKIKKFSILYGLS"
     gene            2700..3077
                     /locus_tag="KPH93_003434"
     CDS             2700..3077
                     /locus_tag="KPH93_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690205.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLVPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIIKFQSHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            3182..3555
                     /locus_tag="KPH93_003435"
                     /pseudo
     CDS             3182..3555
                     /locus_tag="KPH93_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1461 a          421 c          438 g         1286 t
ORIGIN      
        1 ttttattgac aaattactaa aaaatgttct atcagaattg aatacaaaag aaagagtatg
       61 tatttatggg atagttgaaa attttgatgt gctacaacta aaattattct attactatat
      121 attttaatta aaagagtact tgtgagttta agtacatact ttataaatat atttttgtga
      181 gctttatttt ataaaaagta tttagactaa gtattttttt aagtattctc ttgttgaagt
      241 tatttttttc ttttttacaa aataccgtaa ataaaaaaaa gactcttaaa agagtctttc
      301 cgaaaaatct atatattata atatagttat attttctgct tgaggacctc tagcgccttt
      361 aactatatca aagcttactt tttgaccttc ttctaatgat ttaaatcctg aagtttgtat
      421 agctgagaaa tgagcaaata catcatctcc actttccatt gatataaatc caaatccttt
      481 ttcattatta aaccatttta ctattccatt attcattaaa aatactccta ttaacctata
      541 tcaagatata cttgatttac ttgtgtatta ccacaaatac tattgtatca tagattaaca
      601 taaatgtcta tttaattata atttttagtt aacattttta tttattctta atatgaaaat
      661 aaatttaaac attttatatt accaatatat taatgtaaat gtagataaaa ctaaaaaggg
      721 ttttctagaa aaaatctgtc taattatact tacttaccaa taccatcact tgatgcaagc
      781 aagattccac aactttttct ccgtactcta ttataaaatc atctaactgt gtatctataa
      841 aatcacaata atcatatgaa ctaccaatat aagcagttaa catatgtgta catttatcca
      901 ttttatctct aaacataatg tatttatccc cctaaaaaac tttcatgaaa aacaaaaaga
      961 aaatatcttt ttggcaactg gctgacataa cttattttat aagcgttagt ccctatagct
     1021 ttgcgtcact aaatttctct agttttgccg atttagtttt attctacaga ttaataatac
     1081 aatagaatta gttattattc aacatatcta gctgaaaatt taataaattt atatacaaac
     1141 gttaaattat ttgaaaaaat aaaaagcccc tctgttagga gtgactcaaa aactgaattt
     1201 aagtttgtat ttaaaacaca tttttaataa tgcaaaaact aatcaaaaat aaacaattca
     1261 tcaataggtg tatctactat ttttgatata tctattgcta attttaaaga aggattatac
     1321 tttcctgcct ctaaacgcat aatagtttct cttctaactc ctactttttc tgctaattgt
     1381 tcttgagtta attcttttaa ttgtctgtat ttttttaggt tacaaatgaa tttggtcata
     1441 ttatttttcc tctaaaatat aaagttttat agcataaaca tttagagtaa ctgcatatgt
     1501 aattgatagt aaaacacaca ttaattctac attcctagac actatagcaa tacaccataa
     1561 aatgaataat tctataatag caaactgtcc tgtaaatgcc atagctgctt tttcatcttg
     1621 tacatacctt tcatctgctt tatcaccatt tatttttgat ataataaagt ttgagaaaaa
     1681 agcaaaaaat ccaatatatg taaggtcagc aatattgcca gatgaaaaat atttaaggct
     1741 taaaaatcca aggaaaccac aaaaacccat aaaaaatttt ttctgaatca ttttttatcc
     1801 tccttagtaa tttaaaaaga gatatatttg tatctttatg atataaatat atctctttta
     1861 tattcttttg tcaatagaaa agcagtatta tacaatattt gtactgcact ataatgttag
     1921 agcaaaaatc tagcgcttga gatgtttatg tatgactaaa tatgatttta atttgaaatt
     1981 aaaagtagct aaattttatt tgaatagaga aggaggagat gttttaatag taaaacaata
     2041 tggagtttat ggccattctc aagttgaaag atgggttaat atattaggag agagtgcact
     2101 aaaaataaaa aaattttcta tactatatgg tttaagttag atgttataaa ctatatgata
     2161 ataactaaat atagtgtaaa ataagtcgct aaatattttg gaataaacaa tagcacagtt
     2221 atagcaacat gaaaacaaaa atttttacat agtggtgttg atgtcttttc aaaaaattat
     2281 ataaactatc ggtataagaa aacaaaatgt gtatacatga atacatattt ttttaaagat
     2341 atatattatt acacagttaa aaaactttca taaaaacaaa aataaaatat gcttttagta
     2401 acttgttagc ataacttata atttatttta taagcattag cctttatagc tttgtgccac
     2461 taaatttctt tgactctaac gatttagtgc tattctacaa ctgaaataat acaatagagc
     2521 tagtcattgt ttggtagaat tgtttgcaaa ttaaacaaat ttatatatat gaatattatt
     2581 ttacacttaa aaaaacagac taaatatgta gaatatttgg taaaacttac ctctagtttt
     2641 tgtcttaaaa agctcctatt attaatttaa gttagacgaa tttaaggaag tgacataaat
     2701 tgaaagaaga aatattaata gaaaaattat taagagcaga attaatagta atggagtatt
     2761 tatggaaaaa ggacactcta gtgcctaaaa aagaaattgt aaaagaaata aaacagattt
     2821 atggatggca taaaagtaca ataaaaatac tactaaaaag gctagttgat aaaaggtatt
     2881 tggctagata tattataaaa tttcaatctc attataaaat aataatagat aataaagaat
     2941 attatacttt taagaaaaag gtactaaaat ctagtaaaag cataaaaata atgcgttcac
     3001 ttaccactac acataaaaat gtaagtaaag agaagttaga cttattagat gagtattata
     3061 gaaacttaaa agaatgatat agtaaaaatc gaatgtaatt attcacctta attcttttgt
     3121 ttgtaaaact ttattgatat agaatttaac atatacatga attggtaaag ggatgtatac
     3181 tataaaaatt agtaaattgc cagaagcgga attaaaagtt atgagatata tttgggaatc
     3241 agacaaggta ttaatatcaa gagaaatcac caagtctatg gaacaaaata tgaatggaaa
     3301 gatacaacta tatttacagt attaaaaaga ttacaaagaa gagagttttt agaaacggag
     3361 aaaatagata agcgtacaca ctataatgta ttagtaaaag aaaaataata tcagagattt
     3421 taaacaaaag aatttttaaa gaatacacat aaaaattcaa tattaagttt aatgtcagca
     3481 ctatgtagta aggatgatga agtagatggt gatattttaa atttattaga agaatatttt
     3541 aagaatttga aataagaaaa tgacaaattg atacataata aataaaatga aggtatgaaa
     3601 ttataa
//
LOCUS       DAEMAE010000284         3596 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEMAE010000284 DAEMAE010000000
VERSION     DAEMAE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.288"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1275
                     /locus_tag="KPH93_003436"
     CDS             <1..1275
                     /locus_tag="KPH93_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1690206.1"
                     /translation="LLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLK
                     YLYCSQNELTSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGS
                     SGSGGGSLSTNEESSEPTSTPSKEKLTGADRNETSVKISQKGWNKADNIVLINDSSIS
                     DALSATPFAKSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLK
                     KKGLNVIRISGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEM
                     PILLTNQNDDMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDRTET
                     NSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVVLVGNGLDNSQ
                     KEFIKNKKFKNITQIGGNGNEKAFNEVENLVK"
     gene            complement(1317..1502)
                     /locus_tag="KPH93_003437"
     CDS             complement(1317..1502)
                     /locus_tag="KPH93_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG1690207.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(2020..2196)
                     /locus_tag="KPH93_003438"
     CDS             complement(2020..2196)
                     /locus_tag="KPH93_003438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690208.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            2913..3590
                     /locus_tag="KPH93_003439"
     CDS             2913..3590
                     /locus_tag="KPH93_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1690209.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNKDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
BASE COUNT         1462 a          476 c          491 g         1167 t
ORIGIN      
        1 cttttagaat tatactgtta tgacaatgaa ataaaagctt tagatataag taaaaatatt
       61 gacttagaaa atttaacttg ttataaaaat aaattagata gtttaaatac aagtaaaaat
      121 aaaaatctta aatacttata ttgttcacaa aatgaattaa ctagtttgga tgtaacaaaa
      181 aatacagaac ttgtaaggct atattgtggg gaaaatagat taaccagctt agatgtaagc
      241 aaaaacacaa aattaaaaga attagattgg tcaaatcaaa aaaaatcaac atcaacagga
      301 ggttcgtcag ggagtggagg aggttcatta tctacaaatg aagagtcctc tgaacctact
      361 tcaacaccat ctaaggaaaa attaacaggt gcagacagaa atgaaacatc agtgaaaata
      421 agtcaaaaag gatggaataa agctgataat atagtgttaa taaatgactc tagcatatcg
      481 gatgctttat cagcaacacc atttgctaag tctaaagatg caccaatatt acttactaaa
      541 aataataact taaataaatt aacagaaaaa gaaataaata gattagaagc aaaaaatgtg
      601 tacatagtgg gtggattaaa gtctgttgat gaaaaagtag tgtctgattt aaagaaaaaa
      661 ggattaaatg taattagaat atctggaaat gatagatatg aaacatcaat aaaacttgca
      721 aaagaattag acaaaaattc taacctgtca aaagtagtag tggtaaatgg agaaaaggga
      781 ctagctgatg cagtaagtat gggagctata tcagcaaaag aggaaatgcc aattcttctt
      841 actaatcaaa atgatgatat gaaagatata aaagacttaa tagctaataa aaacatatca
      901 aaatcatatg taataggtgg agaatcttta tttaataata aagaagtgaa taacacatta
      961 ccatctgtaa ctaaaatagc aggttctgat agaacagaaa ctaactcaaa agtaataagt
     1021 catttctata gcaaagatac acttaatgac ttatatgtag ctaagaatgg aatgaataaa
     1081 caagacgatt tagtagatgc cttatcagta ggagtgcttg caggtaaaac tgaatctcca
     1141 gtagtattag ttggaaatgg attggataat agccaaaaag agttcatcaa gaataaaaag
     1201 tttaaaaata taactcaaat tggtggaaat ggaaatgaaa aagcatttaa tgaagtagaa
     1261 aacttagtta aatagattga aaggtagtaa ctggaaatag ttactacctc ctttttttat
     1321 tcattttcag ttttttcaat aattattttt ccatcctcaa aagtaacaag aacatccctt
     1381 tcatcaggag agatattcat ctctttaatc catgacattg gaagtatcaa tctaggtgta
     1441 aaagagcctt tgccactttt attaaatcca attctaagtt ttctttgctc caataatatc
     1501 attcctttct actattaata aatgccagca ataataaagt aaataccaaa gcaataagag
     1561 ctttaaaagt aaaacttatt attatattaa taatatttaa aactatagtg ataccaagaa
     1621 taatcataca aattctataa aatttattat tcatattaaa ttagaaaatg tgttataata
     1681 ttagaaagaa gctacctaga aggaaggtat tttcctccta ggattgaaac ttataattct
     1741 ttaatcaatc ttagtattgc gagtactaga ttaattaaaa ctaatgtgaa agagaaagca
     1801 tttttaattt tattaaattt gttttctctt ttattttttc ttctttctaa tctccccact
     1861 ttcttacctc ctttctatat ttatattata tcatcacgtg acgtgaaagt caataaatat
     1921 tccatatttt tcaataaagt tgtaaaatat attaatataa aatacaaaat ataataaaaa
     1981 taagtggatg tttacattat acacccactt attttagagt tattcctcat cagatttttt
     2041 tattataatc tcatcttcct taaaaataac tacagcctta cgtttttccc ttgtaaatcc
     2101 cattttttca agttggctta aaggtaaaga aattttagcc gaataagtac ctgaaccacc
     2161 tttactgtaa actattttag attctttttg ctccaaacta ttttcctcct tttttatatt
     2221 gtaatgtaac aaatattaac aataaaatgc ttactattaa tcctattata ttgttgatat
     2281 taaaatgtaa aaataaaagt attatgtcta ttactatagt tatagcaact ataataagac
     2341 agaatttata aactttatca ctcatatttt atagaaaatg tgttataata tttagaaaga
     2401 agtcactagg aagaagatta aattcttcct agcttacaaa actataaatc ttttaataat
     2461 ttaattagtt tatcaattaa atttattaaa atgggtaaga tagagagagc agttttaact
     2521 ttggcgagtt tgctttctct atcttttttt cttctttcta ttctactcat tttccttttc
     2581 accccctttc tatatttata ttataatatg ccgaaccggc aaaagcaata tttatttcca
     2641 tatttttcaa taaatttata ttgacaaact atattttttt acaaataaaa ataagggtgg
     2701 caaaatcaaa cttgccaccc ttttgccacc gaaaataaat aatagaataa atttaaatta
     2761 aacataataa aacataaaca acaaaactga ccttaataaa acattgaaat accaacactt
     2821 gtaaaaaata acaacaataa acaaataacc tagaataata ttacaactta agtaaataat
     2881 aattttgaat aataatttac ggaggtattc atatgtttaa acacgataaa gcattattaa
     2941 aagaagtaaa agtagaaaga ccaaatcctc aatatgctgt tttaatgcaa gaacagttgg
     3001 gtggtgcaaa tggagagtta aaagcagcta tgcaatactt gtcccaaagt tttagaataa
     3061 aagaccctca aataaaagat ttatttcttg atatagcagc agaagagctt agccatatgg
     3121 aaatggtagc acaaactata aatttattaa atggacatga tgttgattat aatgcagtta
     3181 atacaggcga aatagaaaca catgtattaa ctggtctatc accagtctta ataaactcat
     3241 caggagctcc ttggactgct aattacgtta cagttacagg ggatttagta gctgacttac
     3301 tttcaaacat agcttctgaa caaagagcta aggtagttta tgagtacttg tatagacaaa
     3361 tagatgataa atatgtaaaa gaaacaatag atttcttact taatagagaa gaagcacata
     3421 atgctctatt tagagatgct ttaaataaag ttaaagatac aggttcaaat aaagattttg
     3481 gagttacaga agattctaag ttatatttcg atttgtctac tccagggcca aatcacgata
     3541 ctaagataga tattaatcct ccttcttttg aaaaaccttt aaaaaaataa tttgat
//
LOCUS       DAEMAE010000285         3551 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEMAE010000285 DAEMAE010000000
VERSION     DAEMAE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.289"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1119)
                     /locus_tag="KPH93_003440"
     CDS             complement(<1..1119)
                     /locus_tag="KPH93_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1690210.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKATGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLKDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSYAIRNFLKKIDLDIKLSDL
                     GIKKSDIDWLTDNCMKISAANIKRHPKYFNKEQIKEMYH"
     gene            complement(1507..3444)
                     /gene="htpG"
                     /locus_tag="KPH93_003441"
     CDS             complement(1507..3444)
                     /gene="htpG"
                     /locus_tag="KPH93_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG1690211.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISKSDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEVVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFHVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         1111 a          627 c          350 g         1463 t
ORIGIN      
        1 atggtacatt tcctttattt gttccttatt aaaatacttt ggatgtctct taatatttgc
       61 agctgatatt ttcatacaat tatcagttaa ccaatctata tctgattttt taatacctaa
      121 atcacttagt tttatatcta aatctatttt tttaaggaaa tttctaattg catatgaaca
      181 atcttctgca caagttcctc ctagtacctt tgagatatta ccaaatcttt caatatcact
      241 atcccatgaa ctttctataa tagtaggagt caaagctgca agtccttttc catgcattac
      301 atctttaaga ccacttacag gatgctccat tccatgtggc aaggctgtgc ccgctgtacc
      361 aatagtcatc cctccaatag tacttgcaag tgttattttt tcccatgctt ctaaatcatt
      421 aacatcatta tataccttta ttaggttttc tgatataagt tcaatcccat aaatagcttg
      481 tacatctgca agagggtttc ttccattaga aagatatgct tccatattat gagcaagtgc
      541 atcaaatcca actgatgcaa ttatatgctt tggcattgta accataagtt ctgggtcaat
      601 tattgatgct tttgcataca tactattctt ctttatagat tttttatctt tagtttctga
      661 atttgtaagt actgcaaaac aatttccttc actacctgta cctgatgttg ttggaactaa
      721 gattataggt aaagcttctt caccttgtct aattccaaat atataatcat ttaaatctcc
      781 tgggttttta taagaaaaag ctatactttt ggcagcatcc ataatgcttc caccaccaag
      841 ccctaaaaca caatcacatc cagttgcttt tataatttct actcctgaat atatagtagt
      901 tataagagga ttttgttcta ctctatcaaa aacttcatat tctattttag agtcttttag
      961 taaatctatt gttttatcaa gtaatccagt tttctttgta ctatttttac ctgttactat
     1021 caatactttt ttgccatatt tagatacttc ttttcccaca tcattaattc tgcctctccc
     1081 aaacaagata tttactggca aattataatt aaaattcatg ctaaaacctc ctatttctac
     1141 tttagtaata attttatatt tttaacaaag tgtaattatg tatttaaaca taaactaaaa
     1201 aacaatcttt tacttaatta tatgtattat cttattatat atcaagttaa ctatatttaa
     1261 ctaaatagag ttttttatta gaacctactt aaatatttaa aaattaacca aagataaaaa
     1321 aataaagaat ggtctgtctc aaaatagaga taaatatatt tttcattgtt gaaatgatta
     1381 aaataaattt aatttcacat aaaaaaagcg cctcatgaac tgaaaagttc attttgagac
     1441 acttctttat tatcattgat attgaatact ttttataaat ttgcttcaat gtattcaaat
     1501 tcaaccttat aattctatca aaagactcat aagcttatta cttctttgta caaatgaatc
     1561 taactcatct ggctttaatt ctttattaga aagtaaagct aagtctgcta tctggttaca
     1621 aataagcgta atcttatctt tcttttcctc gtcatcttta agagaaacta attttttgat
     1681 tattggactg ttttcattta tcacaagagt cttttcttcc tcaaaattca ttcctaaatc
     1741 cattccagca aatcttgact gcatttctgc cattctaata gattgttctg atactaacac
     1801 catagctgga gtgtcttcat ttttcaatcc ttctacagaa taattcttca ctctttctcc
     1861 gacagcttct ttaaacagac cttctatctt agctatctct tctttgtttt cttcactatc
     1921 tttattttca tttttatctt ttaatacatc agaaaggtct gcatctattc tattaaactt
     1981 aactccattc tctttatatt ccataaatga aataaaatga gtatctattg atgagtttaa
     2041 aacaacagca cttaagtcat attctttaaa taatttaata tattgagatt gctgttcctt
     2101 atcacttaca taaaatacct tattttcatg tttgtcctta catttttcta aataatcttg
     2161 taatgttata tattcatcat caatagtttt aaataatata cattctttta ccttctcata
     2221 gaaactttca tcttttaagc aaccatattt aataaatatt tgtatatcat cccaaaactt
     2281 attatattcc tctctttcat ttttacaaag agattttaac ttatcagcta cttttttaat
     2341 tatatgttta gaaattttgc ttacgtctct gtcattttgt aagaaacttc ttgatacatt
     2401 caatggtaag tctgggcagt ctataacacc ttttaataaa agtaaaaatt ctggtataac
     2461 ttcttttata ttgtcagcca caaacacttg attgttgtat agttttactt ttccttctgt
     2521 taattcaaac tcatttttta atttagggaa gtaaagtata ccctttaagt tgaaaggata
     2581 atctacattt aaatgtatcc agaataaagg ttcatcaaat acatggaata cctttctata
     2641 aaactcttta tactcctcat cagtacagtc ttttggagat tttaaccata gaggattagt
     2701 atcgtttaaa ggctcaatta cttttgcatc tacaacctct tcttttccat cttccttttg
     2761 ttttttagct ttttcttctg cttctttagc ttctctttct aatctttcaa catcttctaa
     2821 atatatttct actggtaaga aagaacagta tttatttatt attcctctta cagtaaattc
     2881 atctaaaaat tctttactat catcatcaat aaatagtgtt atagtagttc ctctatcatt
     2941 tctagtatct gatttagata tttcgtattc tgtaccacct tcagatatcc atcttacagg
     3001 agtcgctcct tctgtatatg aaagtgtatc tatttgtacc tttttagaca ccataaatgc
     3061 agaataaaat cctaatccaa aatgacctat tatgtcattt gactcttcca tcttatcctt
     3121 atatttatta aagaaatctt cagcacctga aaaagcaact tgatttatgt atttttttat
     3181 ttcttcttca gtcataccaa tcccattgtc aataaattta agtgtacctt ctcctttatt
     3241 tacagatact gtaattttat aatctgatga cttattttct gatatttctc ctagtgatac
     3301 aagtctttta tgtttactta ctgcatcaca tccattactt ataagctctc ttataaatat
     3361 gtctttatcc gagtataacc attttttaat tataggaaaa atattttctg tatgaattga
     3421 aatactccct ttttcaaatt ccatttttta tccctcctca ttttataatt aatattttaa
     3481 attttttaaa atggtatgtc aatatattat aatcatatta ttttaaaata aaataattta
     3541 tttttcttat c
//
LOCUS       DAEMAE010000286         3548 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEMAE010000286 DAEMAE010000000
VERSION     DAEMAE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.290"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(244..1311)
                     /gene="rsgA"
                     /locus_tag="KPH93_003442"
     CDS             complement(244..1311)
                     /gene="rsgA"
                     /locus_tag="KPH93_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG1690212.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     ASGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1308..1709)
                     /locus_tag="KPH93_003443"
     CDS             complement(1308..1709)
                     /locus_tag="KPH93_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG1690213.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGYLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLSQPPFPLERIIEKLEKEETE"
     regulatory      2505..2601
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2655..2831
                     /locus_tag="KPH93_003444"
     CDS             2655..2831
                     /locus_tag="KPH93_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690214.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3158..>3548
                     /locus_tag="KPH93_003445"
     CDS             3158..>3548
                     /locus_tag="KPH93_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690215.1"
                     /translation="MKKRKIVIITLLLIGVLLLGGILYNLFLVKTANISMLKESWNLD
                     IPIPNKEIEVFDTQDSINGDGQSYFIQEFSEKNFKKVFNLKGGIVVSKDNINEIEKYI
                     DKFKRDSVNINKSNKNKIEEDFKKYKLE"
BASE COUNT         1299 a          546 c          455 g         1248 t
ORIGIN      
        1 atgccttttc ttatcttaca taagaccgct ttataaaaga gcctatcata acaaatatgt
       61 atacgttatc atatgtatat atcattatat aaaaaatcaa actttattaa aaaaattaat
      121 aaattgcctt aaataatacc acaaaccaaa tttgccatca aaatcacatt aaagtaacaa
      181 attgatggca aatttaatat attaatagta tgtagactca tataatgaaa aaaaaaacta
      241 cacttatctt ctaatcttct tatttatttt tgagatattt ttaaatttct tttctttttt
      301 tgctaagtat tcgcttttat tgtctgcaaa tactgcttca gttttaagcc tacgatagga
      361 ttcccatctt ttaggcgaaa gttcttgatg agcaatagct tcccaaatag cacatcctgg
      421 ttctgaggtg tgagtacaat tacgaaaatg acattttccg aaataagctt ctatatctgc
      481 aaaactttta tctaaacctt ctgaaacatc ccacattccc aactctctca tacctggtgt
      541 gtcaataaca ataccaccac ctgaaattaa aaatagctct cgtctagtcg tggcatgtct
      601 tcctttatca tcatctatgc gaatttcctt tgtatttaaa aattctccac ccagcagaca
      661 attaattaat gaagattttc caactcctga agaaccaagt aaggcaacag tacagcctgg
      721 tttaatatac ttagaaatct gtttataacc atctggctct atagatgagg taacaactac
      781 atctacaccc aaggctatag aattaactac ttgtaattta cgatgcacat tgtcacataa
      841 gtctgattta gtaagtacta ctactgggat agctccactt tcccatgtta aagacagata
      901 cctctccaat ctatgaatgt taaaatcctt attcagtgac atgcacaaaa aaacaatgtc
      961 aatattagct gcaaccacct gttcctcatt tacctttcca gaagccttac gcacaaacaa
     1021 gctttttcta tgaagcacat gatgaataat agcattacca tttttattat tgcaacgatc
     1081 caacataacg aaatcaccaa ctgtgggaaa atcagattgt atagtagcct caaaacgtag
     1141 ttttcctgaa acttctgcaa acagtacacc atttacacat acagcttgat aaaaattctt
     1201 ttcctgcgag ataattcgag caaggtataa atcatgatat tccatacttt gagtaataaa
     1261 attcctgttt aggccatagt cttgcaaatt gatttgtttc ataccattca ttctgtttcc
     1321 tccttttcca acttttcaat gattctttct agtgggaaag ggggctgtga cagtggtttt
     1381 aatgcaattg acattaattt cactgaatta tcatcagctg caatacgatt agaactaaga
     1441 tgtccacagc gtgtacaacg atgaataatt gcccactcac cattttttct cacccacact
     1501 ccaataggtt ccatattagc atcacaatct gcagcacggt caccaggaat gatatctaca
     1561 tgaagactat gtaaacaatg tggacaatga tttctatgtt gtgtaccagc tccttcagga
     1621 actacaaggt aaccacaatt tttgcaaata aagttatcca tacaggaatt ttcctgataa
     1681 taagctcgtt ttctattttt ataattcatt tttatttcct ccgtatcttg tattttctac
     1741 aaaaaaagtc cacaggtatt tatatctgcc tttttatgtg gtatacatat ttacctacac
     1801 aaaaacagaa aactcctgtg gactagaaaa tttaatatct tctatttcaa cataattata
     1861 ttcaaaataa tactgtgtaa aattaataaa agtgtagcag attaaagccc actacaccac
     1921 aatttaacaa agtaatatgt aatattatta tttgttgtct aatgttagtt atctataaag
     1981 gcagagtaag acacaacaat caaatgtatc aaaaatacac ttaaaaattc tgattaaaca
     2041 gaacccatat gcgtctccaa ctatttattt gttggttacc attatatata actctgacat
     2101 caagcaaaac actcctttac taataattat tattgaaatt ttaccataaa atataagtat
     2161 gttcaagtat aatttaatat attcatatat ttatgtttgt ctcattttct tattctataa
     2221 aatctagtgg tattttaaaa tttaatgttt acctagtatt aataataaaa attaactgct
     2281 attatttgtt ttctcatttt atgcttatcg ctcaatttat ctaacttaaa ttaatagcaa
     2341 aggtctttta atacaaaacc tacaatcaag tttttccata cattctacat acatagccta
     2401 tttttttgat ataaaataac gttcatattt ataaattcat ttaattttca aataagtata
     2461 ttgaataata agcaattcta ttgtattatt gtaattgtag aataaaacta aatcggcaaa
     2521 actagagaaa tttagtgacg caaagctata gggactaacg cttataaaat aaactatgag
     2581 ttatgtcagc cagttgccaa aaagagatat tctttttgtt tttatgaaag ttttttaggg
     2641 ggataataat atatatgttt agagataaaa tggatagatg tacacatatg ttgactgctt
     2701 atattggtag ttcatatgat tattgtgatt ttatagatac acaactagat gattttatac
     2761 tagagtacgg agaaaatgtg gtagaatctt gtttgcatca ggtgatggta ttagtaagta
     2821 agtataatta aatagatttg gttttctaga gaaccctttt tagttttatc tactttaaat
     2881 agtcttatgt tagaaagcta attaagatag atttctagta ttcataattt tattttttat
     2941 ttaaaataca tataataaag atgtaaattt gtatcaaaga ataaatatga gaacttaaat
     3001 tctcttatct atatgaaaat aaacatctct taaaatgaaa ttctgtaaac ataaatgatt
     3061 tttaagtttg aaggattaga ttaacaaaat tattcttgat gacatacctt caaatgatga
     3121 attaaatcat gttttttcaa aaggttgtga acgaaaaatg aaaaaaagaa aaattgtaat
     3181 aatcacatta ctattaatag gtgttttgtt attaggaggt atcctatata atcttttttt
     3241 agtaaaaaca gctaatattt caatgctaaa ggaatcttgg aatttggata taccaatacc
     3301 taacaaagaa attgaagtat ttgatactca agatagtatt aatggagatg gacaaagtta
     3361 ttttattcaa gaattttctg aaaaaaattt caaaaaagtt tttaatttaa aaggtggtat
     3421 agttgtaagc aaagataata ttaatgaaat agaaaaatat attgataaat ttaaaagaga
     3481 ttctgttaac attaacaaaa gtaataaaaa taaaattgaa gaagatttta aaaaatataa
     3541 gttagaag
//
LOCUS       DAEMAE010000287         3464 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEMAE010000287 DAEMAE010000000
VERSION     DAEMAE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.291"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(169..1185)
                     /locus_tag="KPH93_003446"
     CDS             complement(169..1185)
                     /locus_tag="KPH93_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1690216.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTKHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1368..1730)
                     /gene="spoVAE"
                     /locus_tag="KPH93_003447"
     CDS             complement(1368..1730)
                     /gene="spoVAE"
                     /locus_tag="KPH93_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG1690217.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1744..2757)
                     /gene="spoVAD"
                     /locus_tag="KPH93_003448"
     CDS             complement(1744..2757)
                     /gene="spoVAD"
                     /locus_tag="KPH93_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG1690218.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQKAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGEGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2924..3361)
                     /gene="spoVAC"
                     /locus_tag="KPH93_003449"
     CDS             complement(2924..3361)
                     /gene="spoVAC"
                     /locus_tag="KPH93_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG1690219.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1119 a          635 c          370 g         1340 t
ORIGIN      
        1 ataattgaat aaaattattc actcatatta taaaaactat ctaaatagac aaaggagtac
       61 aatgtactta tatacaccta ctccttttaa tttttattta tattatttgt taaaaaactt
      121 cacataatct ataatatttt taataagctt ttgatactta aaagcttact actttattct
      181 tttaacgcta tttcgtatta ttaatcttgg atataatttt cttggaatgt gttttgtact
      241 ctttttattt attatattta ctaaactatc tactccattt tttataactt gctctcttgg
      301 gtcatctatt gttgataaat gcgacctaaa atatgatatt aattcaatat tattataccc
      361 tattatagaa atatcttctg gtacatttat accataagta gtttttaact cttgtattat
      421 agaaatcgct ggtacatctt gttgtacaaa tatagctgaa gcttttttta ttttatcaat
      481 gtatttttct aaaaattcaa cttgagattc aaaatccatt ttagatttaa tgacttcagt
      541 ctttaaattt gcctcttcca tagcattcaa atatcccttg gttctatctt catgcatttt
      601 tgaactctta tcatctgaag ttactgttat tatatcttta tgaccatggt caattaaatg
      661 cttggtagct atataacctc catacacgtt atcgtcccaa aagaaattgt ctacttctcc
      721 tataaaagat ggtttaaaat atataaatac atgtggaaaa ttattgtctt ttaaaaaatc
      781 atactcttct ttttttattg attgagacac aattactaat ccatcaacca tttgtccatt
      841 taccatttta tatacattac tatcaccact aatgttatta ttaggctgta ttataaaatc
      901 gtatttatta gtctcaatag aatttataga actatccatt atatcactaa aaaactttct
      961 agtatcttga tgattaaaat tatttgaaaa gattacccct atcctgttag tttctttttt
     1021 tgctaaattt cttgcattta cattaaaatg gaaccctagt ctatttgcct cttctactac
     1081 tttctttttt gttttttcag atacattagg attattgttt aagcacctag aaacggttga
     1141 ataactgata cctagattcc ttgcaatatc tttaatagtt accatcttta tcccctactt
     1201 cacttcattt tattttgata atataatact acactatgtt gtaaataatt gtcaacttca
     1261 attatgtaaa cgcttcttta tataaaaaat aggtggaatc taaaagatat ccacctgctt
     1321 tataaaaagt tattatttca aaaacttact atgctcttaa taaagattta tggttttgct
     1381 tttggagtaa atactactga cataatatat ccaaagaata tagctgctgc tattccacca
     1441 gctgtagcag ttgtacctcc tagaaaaatg cctaaaaaac catcactttg tattgacttt
     1501 attactcctg tagcaagtga atatccaaat cctattatag gaactgttgc acctgctgct
     1561 gcaaacttaa ctaatggctc ataaagtcct agaaaagtta aaactacacc agttgttaca
     1621 tacgttacca taacatatgg agtttgcatt ttaaaataat ccatcattac ttgggctatt
     1681 aaacatataa ttccacctac aataaatact cttacatagt ccattaacat attatatcac
     1741 ttcctactca tttactatta ctactgcatg tgcaacacat ggtattgtct gcttttgtag
     1801 tgtacttgtc ggtgaaagta gtgctcctgt tgatacaagc attactttgt taaattcttt
     1861 ttttctcaac ttatcatata tatatcctgc aaacactgta gctgaacacc cacatccact
     1921 tccaccacag tggacatctt gttctttaag gttaaacatc tctataccac agtcagtgta
     1981 aactttcgat atatctacac ctttttcttt taaaaagtct tctgctatct gttttccaag
     2041 agtacctaaa tctccagtag caattatatc aaagctattt gggtcatcat tagtatcttc
     2101 aaaataagaa tagatggtat ctattgctgc tggagccata gctggtccca tattattacc
     2161 atcatcaata ccctcatcta ttactttacc aactgtaaca tactttactt ttggaccttc
     2221 tccattagga gctaataata cactccctgc tcctgtaact gtccactgag ctgtcattgg
     2281 tttttgattt ccaagttcaa gtgggaatct aaattgtctt tctgctgtac aatagtgact
     2341 tgatgtacct gacagtacta aatcagcaaa accaccatct ataagcatag aacctataca
     2401 aagaccttct gccattgtag aacaagctcc atatatacct aaaaatggaa tagccaattc
     2461 tcttgctgca aatgatgctg gcactatttg atttattaaa tctccaccta acatataatt
     2521 tacatctgac aattttttac ctgccttttg tatagctttc tgagatgcag tctctaccat
     2581 tttactttca gctaattccc aagtcttttc accatataaa tcgtcggtca taatcatatc
     2641 aaaataatcc tttagtggtc cttcaccttc cttaggacct actatagttc cagttgatat
     2701 tattgttggt ttattttcta acttgactgt tctttttcca attcttttat ttttcatatt
     2761 ttcacacctt tttaattttg cattttacta ttcaaatttt taatattaat taaaaatatt
     2821 aattaaatct gcacaattat ttttcaatta gttactttag tagctatctt cagattttat
     2881 aacttcttca taattaataa aatttaaatt aaataaaaat catctaaaac atcttaaaaa
     2941 tataataaat aattccacat aaaatagaag aacctatacc ataaactaaa actggtcctg
     3001 caattttaaa tagatttgca cctacaccta atacatagcc ttctctttta tactccattg
     3061 ctggagatac tattgagtta gcaaaacctg ttattggaat tatagaacca gctccagctc
     3121 tttttcctat taggtcgtaa actcctaatc cagttaaaaa tgcccctata aatattaatg
     3181 ttattgatgt tgcagaacta gcacctagtt tatcaagacc tccaactttc atatacaaat
     3241 catttattgc ttgccctatc atacaaataa tcccacctac aataaatgca agtgtataat
     3301 tttttaaata tgtaggtttt gggcttattt ggtctacata ttttttataa tttttatcca
     3361 tactaaacac ctctctaaag tattatttgt taattttaaa aaaataaacc tattatttat
     3421 tttgactatc taaagtttgg tcaaaaaata aaaaagatgc ctaa
//
LOCUS       DAEMAE010000288         3446 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEMAE010000288 DAEMAE010000000
VERSION     DAEMAE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.292"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            43..2154
                     /locus_tag="KPH93_003450"
     CDS             43..2154
                     /locus_tag="KPH93_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG1690220.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVKNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2352..3317)
                     /locus_tag="KPH93_003451"
     CDS             complement(2352..3317)
                     /locus_tag="KPH93_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG1690221.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTIEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNAGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
BASE COUNT         1335 a          456 c          581 g         1074 t
ORIGIN      
        1 atattgtaga aaaaataaat ttaaaaattt aagggggatg aaatgaaaaa aataagtata
       61 ttagtattat cattgataat gacacttact atgtgtagtg taagttcatt tgctgattca
      121 tcaaatgata aggaaatgag agctgcttgg atatctacag tttataattt agactggcct
      181 aagactaaga ataatgaagc caagcaaaaa aaggaataca cagatttatt ggacaaatta
      241 aaaagtgtag gaataaatac agcagtggtt caagtaagac caaaatcaga tgctttatat
      301 aaatcaaata taaacccatg gtctgaatat cttactggta cacaaggtaa agaccctgga
      361 tatgacccat taccattttt aatagaagaa gcacataaaa gaggaatgga atttcatgca
      421 tggtttaatc catatagaat aactatggca gatgagagca tagataaatt gccagcaaac
      481 catccagcaa agaaaaatcc aagctgggta gttaaacatg gaaataagta ttactacgac
      541 ccaggtttac cagaagttag aaagtatata gtggatagta tagcagaagt agttaaaaat
      601 tacgatatag atggagttca ttttgatgat tacttctatc caggagtaag tttcaatgat
      661 actgctacat atcaaaaata tggaaaagga caaaataaag ataattggag aagagaaaat
      721 gtaaatactc tacttagaga tgtaaaagcg tctataaaga gtataaagcc taatgttgta
      781 tttggtgtaa gcccagcagg tatatggaga aataagagta gtgacccaac aggttcagat
      841 acatcaggta atgaaagtta tgtaggtaca tatgcagata caagagcatg gataaaacaa
      901 ggattaattg actacgtagt tccacaactt tactggccaa ttggtcttaa agcagcagat
      961 tattctaaat tagtggcatg gtgggctaat gaagtaaaag gaacaaatgt agacttatac
     1021 ataggacaag gtatatataa gcaaggtcaa tcgagttatg gtggacaaaa tatagcaaaa
     1081 gaaatagtac aacaagtaac attaaataga aagtatagtg aaataaaagg tagtatgtat
     1141 ttctcagcta aagatatagc aaatagtact tctatacaaa aggatttaaa gagtttatat
     1201 tctagtagtg aagaaccggt tactccacca tctaatgtta aagtggagaa attaagaggc
     1261 gacgaaagat atgacacagc agtagcaata agtaaaaaag gttgggctac aaattcagat
     1321 acagtagtat tagtaaatgg gtactctata gttgatggta taacatcaac accacttgct
     1381 acatcaaatg atgcgcctat actattggta aataaggata atatacctac atccactaag
     1441 aatgagttaa aaagacttaa tccaagtaaa gttattttaa ttggaggaaa taactctatt
     1501 ggagataaag ttgagagtga gataaaagat acattgtcaa atgtatcaat aaatagagtt
     1561 ggtggttcag atagatatag tacatcttta atgatagcaa aagaattagt aaaaactaat
     1621 ccagtagaaa aactatatat aacaagtggt acaggtgaag cagattctct ttctatagct
     1681 tcaaaagcag gagaagaaaa gcaaccaata gtattagtat caaaagataa tgtaagtgat
     1741 gaagtttaca attggatatc agatttaaaa gtaaaagatg cttattttat aggtggtaat
     1801 ctaagtatat ctgattcagt aataaataag ctggataagg tcattacaaa tgatgtatct
     1861 aaaaatagaa tagctggaga aaatagagaa gaaacaaatg gaaaagtaat acaaaaattc
     1921 tatccaaatg ctgaatattc atcaatgttt gtaagtaaaa gtaatcaatt ggtggatgca
     1981 ttaacatcag gtcctttagc agctaaatta aaatcaccag tagttatgct tggaaatagt
     2041 gtaacatctg ctcagaaaac tgctttagag cctaagaaaa ctactctagt atacgaagct
     2101 ggagatggta taaatcaaaa cacattaaat actttcttaa acttagtgaa gtaataaaaa
     2161 agttaattta aaatacagag tattttataa atgtagatta aaatgaaaat tatcaggata
     2221 aattagaaaa taaaaaagaa ctatatgcat tttgtaatca agtgtacaca gtaacttgaa
     2281 aataattatg cgatatagtt cttttttagt aattaatctt atacttttta ataaatttta
     2341 tgttgaattt tttattgttt aaactctatg tcattaaata taaaatcgtg taatatgtct
     2401 aaagtatctg ggtcaaatct aagaacccat ccagcttttc catatatacc acctgtgcta
     2461 tgaatttcat catcaatagg aaattcaaat tgcttaatat ttaagtcacc catttttaaa
     2521 acttgagctc ctaaagaaag tattgcatta ggtttcatgt tagttttaac gtatggaaga
     2581 actgcatcta ataaacctgg atatttagta acaggtaaag tttccgtttt cttgattata
     2641 gcttgtataa tctgtctctg tctgccatct ctagcaaagg ctgtatcgtt gtgtctgata
     2701 cgtgcaaaag atagtgcctg atatccattt agagtctgct ttccagcatt tctaatgtat
     2761 tgtatgctac ctttatcatc acttttattc catttataag tttcaggaat aaatttgttt
     2821 agttctcgta tttctccttt ttcaatggta acctcaacgc caccaatagc atcaattata
     2881 tacataaatg attcgaaatt aacacaagca taattttgaa tatcaatatt aaaattctct
     2941 tcaattgttt gtattaataa atcagcttgt ccataagcat aagcatgagt aagtttttgt
     3001 ttaccatgac cagggatgtc tacatagcta tcacgagcta atgatgtaag tttgatatca
     3061 ttgtgtttgt tatcaatagt aagtatcatc atagcatctg accttgaaga atcttcattt
     3121 ggtctagcat ctgttcccat aagtagtatg ttaattatcc catcttcatt tttatggtca
     3181 ttgttatcca ataaatcact acttatgctc gagtcatgaa tagcagataa tttataataa
     3241 aaatatccat agacgcttat agggaatatt actaccaaga aagccagtag tataacaaat
     3301 ttctttaatt ttgacaaaag taccccttct ttcttctttt tcctgtatat tttttaatag
     3361 catacactta attatattaa agtattgaaa tatattcaat acttgtttca gttacaaaaa
     3421 aaagacaatg atgtaattta taagaa
//
LOCUS       DAEMAE010000289         3446 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEMAE010000289 DAEMAE010000000
VERSION     DAEMAE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.293"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..1174
                     /locus_tag="KPH93_003452"
     CDS             134..1174
                     /locus_tag="KPH93_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1690222.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLACLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(1639..2994)
                     /locus_tag="KPH93_003453"
     CDS             complement(1639..2994)
                     /locus_tag="KPH93_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG1690223.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(3020..3289)
                     /locus_tag="KPH93_003454"
     CDS             complement(3020..3289)
                     /locus_tag="KPH93_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG1690224.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         1036 a          620 c          465 g         1325 t
ORIGIN      
        1 caatataaaa ccaattaaga tttaaaataa cttttgttct gttgacattt atttaggtaa
       61 ttatttacga atcaggataa cgttatcatg gcaaaagttt atgtcctaaa aaaaatacaa
      121 ggaggatttt cttatgtcag aaattttatt aggaacaggt ttacttttat tggttctatg
      181 ttgtttcaca atttttagtt acaaggcgcc ctatggcatg aaggctatgg gagctctagc
      241 aaacgctgca tgcgctactt tcttagtaga ggcattcaac ctttctttac ttggtgatgt
      301 tttaggtatg gaatttttta gaagtgttgg agctgctaat ggtagtcttg gtggagttgc
      361 tgctgctata cttgtgcctt tagccttagg tgtatctcca gtttatgcag ttttaatagg
      421 attagcttgt ttagattttt ctatattatc tggattcgtt gctggatact ttgtttcttt
      481 cttaattaag tttatagaga aaaaagtgcc acctggagtg gatttagtgg ttgtaattct
      541 tctatgtgca ccactatctc gtcttatagc atcaaatgta gacccattag ttaatagtac
      601 actactacaa attggacaag ttttgataga agctaccaac attagcccta taataatggg
      661 aattatacta ggaggtctaa taacagtagt tggaacgtct ccacttagtt caatggctct
      721 cacatctata attggtctta ctggtcttcc tatggcaata ggagcattag gtgcttttgg
      781 ttcagctttt atgaactcaa cgctatttgc aaaattgaaa ttaggttcta aaaaagatat
      841 tatatctgtt gctattgaac cattaaccca atcagacata atttcagcta atccaatacc
      901 tatatattct actaatttct ttggaggagc cttttctggt atcattgtag ccttgatggg
      961 tcttgtaaac atgacaccag gtacagctac acctgtggct ggttttgcta taatgtttgc
     1021 ttataatcca gttcaacaag ttcttataac agctggttta tgtgctttat tcaatgtaat
     1081 tgcaggattt ttaggttcaa aaatattcag tacctttaaa gttcttactg ctggtgaaat
     1141 acgtggtact gatgtagagg aagaaattgc ttaaaaaaca ataagattta taaatttata
     1201 tctcaagtag tagagtttta tattgtaggc aattttatta attgcaaacg ttttattttt
     1261 tatttatgtt tatgattatt atgtgtgttg aatatttaga taaattttta tttattattt
     1321 ttatttagct ttttgtttag ttttttattt ggttgatata ttaggaataa aaattaatat
     1381 agtttattac tagttaggta gcataacttt ttagatacat aaaaaatttt atattaatta
     1441 gatattatta aaataaaagc gatacatgaa tttttaaatg tccatgtatc gctttatttt
     1501 ttccacaatc ttaacaattg atttttaaag tttattatct taacaaagtt tattatctta
     1561 actataatat ttaaatgcat agactttttt aatttaacca tctaaatatt tagttaaata
     1621 ttattatatg taatataact aatttttaaa tgaatgtata ggagctggaa tccttcctcc
     1681 tctttgagta aataattcag atgaatttgt atttataggc attactggtg ctcgtcctaa
     1741 aagtcctcca aattctacca tatcacctac atcacatcct ggggctggta taattctaac
     1801 ggctgtagtc ttattattaa tcatacctat tgccgcctca tcagctatca tagctccaag
     1861 cgtacctgct gtagtatttc ctggcacagc tatcatgtca agacctacag aacaaatagc
     1921 agtcattgct tcaagcttat ctatacttaa agcaccttta ataacagcat caatcatacc
     1981 tgcatcttca gatactggta taaacgctcc actaagtcct ccaacatgtg aacatgccat
     2041 tactccacct tttttaacag catcatttaa aagtgctaat gctgctgtag tcccatgagt
     2101 accaacaact tcaagaccca tttcctctaa tatatttgct actgaatcac caatagcagg
     2161 tgtaggagct agtgataagt ctattatccc aaatggaacg tccaatctgc tagaagcttc
     2221 cttcgccaca agttgtccaa ctcttgtaat tttaaatgca gtctttttaa ctgtttctga
     2281 aacaacatca aagggctctc cctttacttt ctctaaagct ctcttaacaa caccaggtcc
     2341 acttacacca acatttataa ttctatcagc ctcaccaaca ccatggaaag ctcctgccat
     2401 aaatggatta tcttctactg cattacaaaa tactactaat tttgcacaac caaaaccttt
     2461 tgcatctttt gttaattcgg cagtttcttt tatgatttct cccatttctt tcactgcatc
     2521 catatttata ccacatcttg tagaacctac atttactgaa gcacatactt tattagtact
     2581 tgctagagct tttggaattg acttaattag tatcttatca ccttttgtat atcccttttg
     2641 aactaatgct gagaatccac caataaaatc tatacctaaa tcctctgcag ccttatccaa
     2701 tgtctgggca aactttacat agtcattttc atctgttgca cctgcaataa tggaaattgg
     2761 agttactgat acccttttat ttacaattgg gataccaaat tcacgctcta tatcttttcc
     2821 aaccttcacc aaatctttag cagaactcat aatcttattg tatatttttt ctctagcttt
     2881 atctccatca gggtctatac aatctaataa agaaatcccc atagttatag ttcttatatc
     2941 caactgttct tcatctatca tctttattgt ttcaagtata tttcctacat tcactattat
     3001 cacctcccct tactgtctac tacagattat gcattgaatt aaatatttct tctcgctgaa
     3061 tttttacatc tactgataat ttcttacctc tttcaaccaa gtcctcaacc gttttctcaa
     3121 aagaatttaa tgatttctct aaatcaacta tcataatcat agtaaaataa tcctgtaata
     3181 tcttttgtga tatatctact atgttgatat tttgcttgta taattcatca ctaattcctg
     3241 ctactatacc tggtttatcc tttccaatta ctgttaaaat tgctttcata ataatatcct
     3301 cccttatttt tttataaatc ttatattttt acaaattgaa ctatgtatta acatatattt
     3361 catttatttg ttagtaaacc actttttatg tatatattta atatatttta atacctatac
     3421 attcatttgt caattttttt tcaata
//
LOCUS       DAEMAE010000290         3428 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEMAE010000290 DAEMAE010000000
VERSION     DAEMAE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.294"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..769
                     /locus_tag="KPH93_003455"
     CDS             35..769
                     /locus_tag="KPH93_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1690225.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            779..1912
                     /gene="nagA"
                     /locus_tag="KPH93_003456"
     CDS             779..1912
                     /gene="nagA"
                     /locus_tag="KPH93_003456"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG1690226.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIEFDFTREIKRCTDIVLSIGHSNANYEQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTICLGEVVYKNI"
     gene            2010..2759
                     /gene="nagB"
                     /locus_tag="KPH93_003457"
     CDS             2010..2759
                     /gene="nagB"
                     /locus_tag="KPH93_003457"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG1690227.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDNKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIIDEAAASKLNPKDYKLV"
     gene            complement(2891..3310)
                     /locus_tag="KPH93_003458"
     CDS             complement(2891..3310)
                     /locus_tag="KPH93_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690228.1"
                     /translation="MKKALRYLLFLIILLLMYFLAVKLNYDFYTQFHTGYMQDFKRYI
                     FINLISSGGIGLLLGTELLIREYKNDGSWYIDIPRLLLLSFPSFLLSLMPVFFFMFPV
                     GNIPIIGNFIMLDRIPLNIIIFNILFGYFLITSFRKK"
BASE COUNT         1422 a          371 c          595 g         1040 t
ORIGIN      
        1 attggtatat accatatcga aaagtggtga ctatatgaaa atagtaaata aatctagtaa
       61 cataccattg catactcaat taagttcaat aataagagag atgatagaaa ctggtgagct
      121 taaagagggt gatgcaataa tgccagaaag agaactttgt aacatacaaa atgtaagtcg
      181 gatgacagta aacaaaacca ttgttggatt agtcacagaa ggacttcttt atagagtaca
      241 aggtaaaggg acattcgttg caaagcaaaa aaagaagtat cagttttcaa atgtaaaagg
      301 ttttactgat gtcatgaagg aaaaaggagt aaacataaaa actgatatat tatcctttga
      361 gatggaacta ccagatgatt tagttaaaag aaaacttgga ataagtgata atactacaaa
      421 tatatataag attgtcagac ttagatatac tgatggagag ccatttggat tagaaatagt
      481 atatctatca gaagaaatgt gtaaaggtct aacaaaaggg atattggata attcttcatt
      541 atatagggtt ttaaatgaaa aatatggcta taagatacaa aaagctgaac aggtaatgga
      601 gccagtaata ctttcagatg aagaaagtaa gcttcttgaa acagatgaag gtgctttagc
      661 tcttaaactt catagaaatt cctataatag agaaggtagc cctatagaat ataccatatc
      721 aatatttaga acagataaat atcaatatga aatagtattg agtgagtagg tgaataatat
      781 gaaatgttta attaatggaa agataatatt aaaaaatcaa atcttggaaa ataaagtatt
      841 agtatttgat gaaaaaataa ttgatatagc tgatagtgta ccaaaagatt gtgaagttat
      901 agatgctgat ggaaaatata tatctccagg gcttattgat attcatatac atggaaacat
      961 gggaaaagat acaatggatt caactgatga atctattgaa acaatttcaa aatcaattat
     1021 gagacatgga gtaacatctt ttcttccaac tacaatgact atggataaag aacatgttta
     1081 tgatgcactt gaagttatta aaaaagccca aaatagaaag cttgaagggg cacaagtatt
     1141 aggagcacat ttagaaggac cttttataaa tgaaaattat aagggagcac aaaatgaaaa
     1201 gtttataata aattcaaaat atgaatttat aaaagaatac aaagatgtta taaaagttat
     1261 aacttatgct ccagaaaaag atattgaatt tgattttact agagaaataa aaagatgtac
     1321 agatatagtt ttatctattg gtcatagtaa tgcaaattat gagcaagcta aagaagctat
     1381 aaatttagga gtaacaaatg taactcacat gtttaatgca atgactggtt taaatcatag
     1441 agacccaggt gttgttggcg cagcacttac aactaatgtg tacagtgagc taatagcaga
     1501 tacaatccat ataaataaag atttatttca gtttatacta aacaataaag ggaaagaaag
     1561 actgatttta ataacagatt ctatagaagc tggtggtctt gaagatggca attattctct
     1621 tggaggtcag aaagtcatag ttaaggggaa tgaagctaga cttgaaaatg gagcattagc
     1681 tggaagtgtg ttgtctttaa ataaaatggt atttaacttc ttagataata caaaccttaa
     1741 agtgaatgaa gcaattaatt tagcatcttt aaatccagca acatctttgg gaataaatga
     1801 caaaaaagga agcttagaaa taggtaagga tgcagatata gctgtgtttg atgagaattt
     1861 agattgtaag atgacaattt gcttagggga agtagtatat aaaaatatat agtatacaaa
     1921 acaaacttta aagacagtaa aagcaatata aaaaataata aattaaactt acattagtta
     1981 aagtagtagt taaaatttag gaggaaaaaa tgagagtatt agtgtgcaaa gattatgatg
     2041 gaatgagtaa aaaagcagct gagatgatag cagctcaaat agttttaaaa ccaaattcaa
     2101 tactgggact agctacagga agtacaccag taggtatgta tagagattta gtaaaaaaat
     2161 acaatgataa tatagtagat ttttcagatg tgatgagctt taatttagat gaatattata
     2221 agttaccaat atctaatgac caaagttatg attattttat gaaagagaat ctatttaatc
     2281 atgtaaacat aaagccagaa aatactcatc taccaaatgg aatggctgat gatattgaaa
     2341 aagagtgcat gaactatgaa gcttctatag atgcagctgg tggtatagat gtacaagtac
     2401 ttggaatagg tcgtaatgct catataggtt ttaatgaacc tgataataaa tttgcaaaaa
     2461 gaactcatgt agttgaactt acagaatcta caatagaagc taatgcaaga ttctttaaat
     2521 caagagaaga tgttcctaaa aaagctgtaa gtatgggaat aggttcaata ttgaagtcta
     2581 agaaaatatt gcttttagct tcaggagaag aaaaagctga tgcagtatac aatactgttt
     2641 atggagatat aactccagaa gttcctggtt ctatacttca acttcataaa gacactatag
     2701 ttataataga tgaagcagca gcaagtaaac ttaatccaaa agattataaa ttagtataag
     2761 aatagaatgt agtttaagtg aaaaatagct atcattgaat aatcaaattt atttaatgat
     2821 agctattatt atttaaatta cttacttctt tttatttaaa ttagttatta ttgtttaaat
     2881 tacttgttta ttacttttta cgaaaactcg ttatcaagaa gtatccaaat aagatgttaa
     2941 aaattattat gtttagtggg attctatcaa gcataataaa attacctata attgggatat
     3001 taccaactgg aaacataaag aagaatacag gcatcagtga tagtaaaaaa gatggaaaac
     3061 taagaagaag taatcttgga atatcaatat accaactacc atcatttttg tattctctaa
     3121 tcaataattc agttcctagt aataaaccaa taccaccaga ggaaattaaa ttaataaaaa
     3181 tgtatctttt aaagtcttgc atataaccag tgtgaaattg agtatagaaa tcataattta
     3241 atttaacagc taaaaaatac ataagcaata gaatgattaa aaataaaaga tatctaagag
     3301 cttttttcat tttaaattct cccttcagaa atactttatt cagttttatt ttgttggaat
     3361 ctgattttat cacttagttt aagaaatcca taatattagt gaagaatgaa ataagtatta
     3421 tatatgaa
//
LOCUS       DAEMAE010000291         3426 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEMAE010000291 DAEMAE010000000
VERSION     DAEMAE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.295"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..454
                     /locus_tag="KPH93_003459"
     CDS             119..454
                     /locus_tag="KPH93_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690229.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNIVESTYFLSESGLLLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            591..1868
                     /locus_tag="KPH93_003460"
     CDS             591..1868
                     /locus_tag="KPH93_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG1690230.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTVFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNLKFKEMRAITAFLLGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEDNKRENSTDKEENSDGNDSQKDSNNENNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKGQSYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTKYIPGAMDKATMKSFIENQRNK"
     gene            1989..3377
                     /locus_tag="KPH93_003461"
     CDS             1989..3377
                     /locus_tag="KPH93_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1690231.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYGKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSKLAIVEYEVDGKSKELASNMILVATGRKANTEGL
                     GLENAGIELNKRGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIIINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRERVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1415 a          325 c          601 g         1085 t
ORIGIN      
        1 aataggagta tataataaaa attttaatta aataaagcta ggcatattta taatattaat
       61 aaatatattg ctataaaagt ttgtttaata aattaaataa agaaaagagg agaataaaat
      121 gtacaacata gatgatatga tatataattg tccggtagaa gctttatcaa atattttagg
      181 taaaaagtgg gtggctataa ttatatggaa aatacaagaa gataaaaagc gatttgggga
      241 gttacaaaga gctataccag actgtagtaa gaaaatgtta gttcaacaac tagatttttt
      301 aattgaacag ggtattatag tcaaccaaaa aaagttggtt aataatattg tagagtcaac
      361 atatttttta agtgaatcag gattactatt gcttcccgtc atggaaaaaa tgattatgtg
      421 gagcaataaa aatttaaatt gtgataaata ctaaaattta atctgtaagt aatgaaaatt
      481 taattaagct atgagtagaa aaatgtatta aaattatata attttttagt tagattttaa
      541 tttagaaatg ggattgtaat attggaaata aaataaggag gaattttaat ttgcaaaatg
      601 taaatttatt tttagtattt atagaaggta ttgtatcatt cttttcacca tgtatactgc
      661 caattttgcc tatttatttg agtatattgt caaatagtag tgtagagaat ctaaaagaag
      721 gaaagacgag ttttatagga agttctctat ttaaaaatac tgtatttttt gctcttggaa
      781 tctcgacaac attttttata ttgggttcat cagtaaaagt attgagtatg ttttttaatg
      841 aaaataaaga tttaataatg tttattggtg ggattataat tattatcatg ggattatttt
      901 acatgggaat aattaaatct tcaatattga atagagaaaa aagatttaat cttaaattta
      961 aagaaatgag agcaataaca gcttttctat taggttttac atttagtttt ggatggacac
     1021 cttgtattgg tccaatattg gcatctgtat tagtaatggt ttcaagttca agtaatcatc
     1081 ttagtgccaa tcttttaata gcagtatata ctattggatt tattttgcca ttcataatta
     1141 cagctatgtt ttacagtaaa ctatttaaaa ctatagataa gataaagtct aacatggaaa
     1201 ttattaaaaa aataggtggg attatactta tagtatcagg aatattaatg atggtaaatg
     1261 gatttggaag tataagcaaa cactttaata cgtcacaaaa tagtaaaatt gaaagcaagc
     1321 aagaagataa taagcgagaa aattctactg ataaagaaga aaattctgat ggtaatgata
     1381 gtcaaaaaga ctctaataat gaaaataatg ataaagggtc taatgatgaa gatagaataa
     1441 agtctattga ttttacttta acagaccagt atggtaaaac acataaatta agtgattatg
     1501 agggcaaagt tgtattctta aatttctggg ctacatggtg tcctccatgt aaagaagaaa
     1561 tgccttatat agaacaactg tataaagact ataataaaaa taatgatgat gtagtaattt
     1621 taggagtagc ttcacctaat ttaggtagag aaggttcaag ggaacatgta gttaacttct
     1681 taaaaggcca aagttataca ttccctgttg tacttgatga agatggagca ttagcatatc
     1741 aatatggaat taatgctttt ccaactacgt ttataattga caaggaagga tatgtaacta
     1801 agtatattcc aggagctatg gataaggcaa ctatgaaatc atttattgaa aatcagagaa
     1861 ataaataaaa gtatttaagt ataagtttat aaatcaattt aaggttacaa aaaagtgcct
     1921 aattgtacca cccaaataat cttgttatag taaaagtata aacaatacaa gaaagggttg
     1981 aaatttatat gaaaaaaaca tttgatgcta taataatagg atttggaaaa ggtggaaaaa
     2041 cattagcagg ggatttggca aacaggggat taaaagtagc tttaatagaa aagtcaaaca
     2101 agatgtatgg tggaacatgt gtaaatgtgg catgtatacc aacaaaaagc ttagaaaatt
     2161 cagcaaacag tgtaaaaaca aagaatataa attcttggga tgaagtacaa gctgaatatg
     2221 ggaaagctat agataaaaaa gaaactttaa tcacaaaact cagagaagct aactataata
     2281 aattaaattc aaatgagaat gtaactatat tcacaggaat gggaactttc attgatgaaa
     2341 aaacagttca agtcaaaact gaaaatgaaa tttatgagtt agtagcagat aatatattta
     2401 ttaatactgg ttcaagacca tttataccta atattaaggg aattgaaaat aagaatatag
     2461 tatatgatag tgagtctctt atgaatctaa gagttttacc taaaaaaatg acaattatag
     2521 gagctggatt tataggttta gagtttgcgg gtatttatag ttcatttgga gcagaagtta
     2581 caatattaaa ctctaataat ggcattttac caaatgaaga tgttgaggat tcagaagaaa
     2641 taataaaatt acttgcaaag agaaatgtaa aaattgttaa taatgcaaat ataaaagaga
     2701 ttaaagaagt ttctaaatta gcaatagtag aatatgaagt agatggtaaa tctaaagaat
     2761 tagcaagtaa tatgatactt gtagctacag gaagaaaagc gaacactgaa gggctagggc
     2821 tagaaaatgc aggtatagaa ttaaataaaa gaggatttat taaagtatca gaaactctta
     2881 agactaataa agagcatata tgggctatag gagatataaa tggaggacca caatttactt
     2941 atatttcatt ggatgattat cgtataatta ttaatcagtt atttggagat aagactagaa
     3001 caactaatga tagaaagaat ataccaaatt ctatatttat aagtccagca ttttcaagag
     3061 ttggtcttaa tgttaaacaa gctaaagaaa agggatatga agtattagtg gcaaagatgc
     3121 cagtagaagc aattccaaga gctaaacaga ttggcaaggc agatgggttt ataaaaatag
     3181 tgatagataa aaaatccaat aagatacttg gagctagtat gatatgtgaa aattcaagtg
     3241 agattatcca tcttattcaa ttagctgtag atttagaagt agagtataca tatttaagag
     3301 aacgtgttta tgcacatcca actatgacag aagctcttaa tgatattcta tctcctaata
     3361 tgataaaaga agtataacat aagtttaata tataaaccta aaattatcat tataaataga
     3421 ttttag
//
LOCUS       DAEMAE010000292         3418 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEMAE010000292 DAEMAE010000000
VERSION     DAEMAE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.296"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            173..1300
                     /locus_tag="KPH93_003462"
     CDS             173..1300
                     /locus_tag="KPH93_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1690232.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPAFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            1404..2039
                     /gene="lexA"
                     /locus_tag="KPH93_003463"
     CDS             1404..2039
                     /gene="lexA"
                     /locus_tag="KPH93_003463"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG1690233.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(2186..3334)
                     /locus_tag="KPH93_003464"
     CDS             complement(2186..3334)
                     /locus_tag="KPH93_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1690234.1"
                     /translation="MIERSIKNSKLKTLILLLIVSISIYLTGCATSDNGNNQSNNNDS
                     LSSSISSDKNVKIHFINTGNSDAILIQDGKTFTLIDGGDNDDEKLMVDYLNNQGVKDI
                     KYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKDLSPSVPL
                     ENNKFYLEDSYFEVLNTNGGDSTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILPKLE
                     KVDLLKVAHHGSRSSSSQEFLDKINPEYAVLLVGKGNSYGHPHQETMNKLKKMGVKVH
                     RSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNNSFKNKQNTSNS
                     EEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
BASE COUNT         1252 a          476 c          487 g         1203 t
ORIGIN      
        1 ttaactagtc tttaaatgtg gatttaggga atagttagtg gcgatttcat ctaaagcact
       61 gctaatctct taaaaataca aaaaatgatt aaattaaata tttatactca cttggtataa
      121 atatcaaaaa ataatataaa agatttatat tttagtttta ggaggtttaa ttttgaataa
      181 tgataagaaa attataaaag tacataataa atcagataaa cctgataata ttgtcatcaa
      241 agaaaatgaa ctcatagaaa aatgtctact tttggaaaat aaacttcctg cttttatgaa
      301 ggattatttt atatatctaa aaggttctgt tgcagtttct acacgacttg cttatttaga
      361 agatattaat tttttctgtt catatttgat tgaaaccaaa gaattaacaa atgcagattg
      421 tgtaaaagat attacagaag atgattttaa tacaataaaa tcaagagata taaatttatt
      481 cttaggtgat tattgtagta gatattataa aaatacagaa aaaaatacat tgatttttga
      541 aaataacaat agagctttag caagaaaaaa atcatccatc tctactctat ttaaattctt
      601 atatagaaac tctcagatag ataataatat aactgatgga ttcaatccaa taaaacttcc
      661 taaacctcag ccagatgcta ttaagcgtct tgaaatagat gaagttgcta agatgcttga
      721 atcagtagaa acaggtgaag ggcttacaga gaaagaaaag gtatattgga gaaagactaa
      781 gttacgtgat aaagctatac tagcattgtt tgttacatat gggcttagat taaatgagct
      841 tagagaactc aatatctcct cttttaattt ttcaagaggt gaatttaaaa tatatagaaa
      901 acgcggtaaa gaagttttaa tgcctataaa tcatacttgt gagcatgtaa taaaagatta
      961 tttgcaaaac gaaagaacaa gagatgaact attaaatgat gaagaaaaag atgctttatt
     1021 tttgtctctt caaaataaga gaattacagc aaaggctata agaacactgg taaaaaaata
     1081 tacatctatt ccacttgata ctactagaga caatggatat agtccccata aattaagggc
     1141 aacggctgca acttctctaa ttcaaacagg attttctata tatgatgttc aaaacttact
     1201 tgaccatgat aatgtcacaa ctactcagct ttatgcagct cataagaaaa atgtaaaacg
     1261 tgatatagtc aagaattttg aatggattga ggacgattaa aaatcaggaa caaatgtttg
     1321 cagatataaa tggattatgt tataattaaa aatataaagt gtatggaaat aaaaacaaaa
     1381 tatgtttgga aatgagtgat gttatgtatt tagatctaac tgaaaagcaa gtcttgatat
     1441 tggaatttat aaagtctcaa atcacattaa aaggttatcc acctgctgta agagaaatat
     1501 gtactgctgt agggttaaga tctacttcaa ctgtgcactc tcatctaaat aaacttgaaa
     1561 aactgggata cataagaaag gaccctacta aaccaagagc tattgaagtt ttagaacgta
     1621 gtaaagtaaa tgatgtttct ggagctaatc aagaaataat agagcttcct ttagtaggtc
     1681 aaataacagc tggagaaccc attttagctc aacaaaacat agaggaatac attccattcc
     1741 ctgccagttt agtaaagggc agtaacaatt ttgtattaag agtaaaaggc gaaagtatga
     1801 ttaatgcagg aattttagat gaagattacg ttgtagtaga caaaaaaaat acagccttaa
     1861 attctcagat agtagttgca cttataaatg gtgaatctgc tacagttaag aggtttttta
     1921 aagaaggaaa tttaataaga cttcagccag aaaatgattt tatggaacca attatgctca
     1981 atgactcaga agttgaaatt gtaggtattg taactggtgt atttagagtt attaaatagc
     2041 tattaaaaaa tacttaaaaa aataagctat tcgtatagat aaaatgtgtt atctattgat
     2101 tatagcttat ttttttatgc atttatttta ttttttaaat gatttaaagt ttagaagctt
     2161 tatagataaa ttggttgctt ttttgttatt ttccataaca tatatcacat gggtcatttt
     2221 tcttactttc tgcaattgtg cctgataata tttttttact tcttgaaagc ccactacaat
     2281 tttttgtaga gtgataactc ttaccttttg gagtaaaata cactatttct tctgagttgg
     2341 atgtattttg tttattttta aaagaattat ttttacttgt attactgttt gattttccag
     2401 aatttccatc ttctctaacg cctttattat aacttccatc tttacaactt gtaaatacgc
     2461 catcacctgt tgattcaaat attatatctg aacactcatc acttctatgt acttttactc
     2521 ccattttttt taatttattc attgtctcct gatgtggatg accatatgaa ttaccctttc
     2581 ctactaaaag aaccgcatat tcaggattta ttttatccaa aaactcttga gatgaactac
     2641 tacgagaacc atgatgagct acttttaaaa ggtctacttt ctcaagctta ggtagtattt
     2701 ctttttcggt accttcttca gcatcacctg taaataaaac tttatcattc ccattagtat
     2761 atacaaggac tagagattgt tcattagtac tatctcctcc atttgtattt aaaacttcaa
     2821 aataagaatc ttctaaatag aacttattat tttcaagtgg tacacttgga gataaatctt
     2881 tatttgctaa agcatttata aagtccctat aactctttgt ttctgctgaa ccattcgata
     2941 caaaaacatt ttctatattg aagtttttga caactgaatc aagtccaccc aaatggtctg
     3001 catgagaatg ggtagctata agatatttaa tatcttttac accttgatta tttaggtaat
     3061 ctaccattaa tttctcatca tcattatctc ccccatctat caatgtaaaa gttttaccat
     3121 cttgaatcaa tattgcatca ctgtttccag tgttaataaa atgaattttt acattcttat
     3181 cacttgatat actactagac aaagaatcat tattatttga ttgattattt ccattatctg
     3241 atgtagcaca accagttaaa taaatcgata tagatactat taataaaagt atcaaagtct
     3301 taagtttaga attttttata cttctttcta tcacaaacta cgcctccata ttcataatac
     3361 tctagtaatt ataacatgat atgcgttttt atgatatttt tttacataat attttaaa
//
LOCUS       DAEMAE010000293         3402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEMAE010000293 DAEMAE010000000
VERSION     DAEMAE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.297"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..1333
                     /locus_tag="KPH93_003465"
     CDS             119..1333
                     /locus_tag="KPH93_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG1690235.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CISALEELDTFIELYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1510..>3402
                     /locus_tag="KPH93_003466"
     CDS             1510..>3402
                     /locus_tag="KPH93_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1690236.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGARIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLNKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRILQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSNKYNKTPLIITPNIKKVLLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKISSEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLK"
BASE COUNT         1411 a          380 c          549 g         1062 t
ORIGIN      
        1 taaaatgtta tcacatttta tctatacata cccatataac tagataatta aaatgtataa
       61 tattatttgc tatagaaaat aatacactca tacataataa attttaggag tgaagtatat
      121 gaatcaagaa tatgtaaaag gaaatcctat ttttactaat attaataaag ttccaagaca
      181 atttccatac cttactgatg atattgatac tgatgttata attgttggtg gtggcgtaac
      241 tggatgtatt tgtgcttact atctagctaa aaacaatata aagtctgtca ttcttgaaaa
      301 aggaaggatt gcacatggaa gcactagcgt gacaacttct ctattacagt atgaattaga
      361 tgacaatttg atagatttaa cagaggttat gactctaaaa gacgctctaa aagcatataa
      421 cctttgtatt tcagcacttg aagaattaga tacatttata gagctatatg gaaataagtg
      481 tgattatgcc aaaagagata ctctacttta tactgccaat aaacttgaaa ttaaagctat
      541 caaagaagaa tataacttaa gaaaagaaaa tggatttgat gttgaatata ttgatgaatc
      601 tacaaacccc tttagctttg atttaaaatc aggattgata gcaaagaatg gaggacgaga
      661 attagaccca tataaatata gtcaccattt gattgatgta agtttaaaaa atggtcttca
      721 agtatatgaa aatacagaag taaaaaaagt agatttatca aatgataagg ttactgctga
      781 agtatcgtat ggatacaaag ttcatggtaa aaaattaatt gtagctacag ggtacaatac
      841 aagtttattt acaaaaagaa attttgcaac taagtcaaat acttttaata tagcaacgaa
      901 accacttaaa aacatagctt cttggaaaaa caacatttta attagagata actgtgaccc
      961 atataattat ctaagaacta ctaaagacaa tcgattaatt ataggtggtg aagatgtaag
     1021 ttttgatgat atagaaaatg aaactttagc caatgaaaag tatgatattt tagaacaaag
     1081 attgaaaagt atgtttaagg atataaaaga cattgaagtt gaatataaat attgtggttg
     1141 ttttgcttca actttagata atttaggttt tattggtcca gataataaaa ataacaatct
     1201 atggtattgc ttaggttatg gagcaaatgg tatactcttt gcaatattag gaggtataat
     1261 gttaagtgaa ttatatcttg gcaaacaaaa taaaaatatg aaattattta aagtagatag
     1321 atttgataaa tagatattat gtattaaaag actgtaaaaa gatgaaactt tataattcta
     1381 aaagttaact ttgatacagt cttttataat gttcctttta aaaatacttt atttataatt
     1441 aaatataaag taaaatatta aaatataaat gataaaattt agtcataaga taatatttaa
     1501 gtttatttga tgaaatatgg aggaattatt atgaaggaaa tactcgttat tgctcccact
     1561 aaaggaacct atgaaaaatc aattcacata gttaaaaaaa acaaatatac aaatattgat
     1621 gttgtatttg gcaatctaaa agaaggtata cctcttgctg aaaaaagtat taatcatggt
     1681 gctagaatta tcataagccg aggtggaact tacaatatgt taaaagctac ttacaatata
     1741 cctatagttg aaattaaagt agatgcttat gatattataa aaagctacaa agaggtaaaa
     1801 aattccaacg aaccatttgg aataattgga tttaataatg tcatctacgg tttcgatatt
     1861 atagaagaaa tattgaataa gaaaataact atgattgaaa ttgaaaaaga agaagaaatt
     1921 tatgatgcta tagaaaagta cagaaagaaa ggtataaaca catatatagg agataccact
     1981 gttgcacata ttgttaagag acttaattgt aagggaatat taatcgaatc tagagaagaa
     2041 aacatactta gagccattca acaagcagaa caaatactcg aagcaaccaa ggatgaacaa
     2101 aaaagaagat tacagataga agctatgaca gattttgtac atgatggaat aataacagtt
     2161 gataaaaatt ttaaaataac attatttaat aagagtgccg aaaaaatatt tggtataaaa
     2221 aaagaaaatg cactttatca taatgttgta gatgttatcc caaacacagt tcttccacaa
     2281 gtagttgaga ctatggaacc tcaaattggt gaaatacaaa atataggaag tacaaaaatt
     2341 attacaaata gagttccaat cattgttgat ggtcagattc aaggagctgt ttccacattt
     2401 caagatagca aggagataag ttcttttgag catacaataa gaaggagttt attaaacaaa
     2461 ggtctatctg ctaaatacac ttttgatgat ataatccatg ctagtgaaaa gacaaaagat
     2521 tgtatagaac ttgcaaaaaa atatgcatgt tatgataccc caatgattat cactggagaa
     2581 tctggtgttg gtaaggagct attttgtcaa agcatacata actatagtaa gcgtaaaaat
     2641 gcaccttttg ttgctgtaaa ttgcgctgcc atacctccat ctttaataga aagtgagttt
     2701 tttggttatg aggaaggttc atttacaggt gctagaaaaa aaggtaaagc aggtgtattc
     2761 gagttagcac atgaaggtac aatcttccta gacgaaataa gtgaaatccc tcttgaactt
     2821 caaggtcgat tattgaggat attacaggaa aaacaagtta tgagaattgg tggtgataaa
     2881 gttattccta tagatgtaaa aattatctgt gcttcaaata aagacttaaa aaatatgatg
     2941 cgcaatggtt tatttagaca agacctttat tttagaataa atatattaac attatacctt
     3001 ccttcattaa aagaaagaaa agaagatatt ataaaattat cagaattttt tatacataaa
     3061 tattcaaata aatataataa aacgccttta attatcactc caaatattaa aaaagtactt
     3121 cttgaacgag aatatgaagg taatataagg gaattggaag gtatgatgga aagggctgtt
     3181 attgtggaat catttgactc tattttatta gaaaagatta gtagtgaata tacaaataat
     3241 atagttgata aagataatag tgattttgaa tgcagtaatt tagatttaaa aactttagaa
     3301 aaaaaatata tctataaaat ttataacgaa aataatagaa atactacaaa aacctgtgaa
     3361 attctaaaga taactcgctc cactctatgg cgtaagttaa aa
//
LOCUS       DAEMAE010000294         3397 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEMAE010000294 DAEMAE010000000
VERSION     DAEMAE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.298"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..975
                     /gene="modA"
                     /locus_tag="KPH93_003467"
     CDS             163..975
                     /gene="modA"
                     /locus_tag="KPH93_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1690237.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKKNKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAQKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            985..1776
                     /locus_tag="KPH93_003468"
     CDS             985..1776
                     /locus_tag="KPH93_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690238.1"
                     /translation="MKNKFFTILLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKILKLDFVFTKQGIILAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNRGVDKN"
     gene            1778..2473
                     /locus_tag="KPH93_003469"
     CDS             1778..2473
                     /locus_tag="KPH93_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1690239.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMHIEDIKSRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2460..3191
                     /locus_tag="KPH93_003470"
     CDS             2460..3191
                     /locus_tag="KPH93_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690240.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLYDNPHDRSLFIASLN
                     AVMKYLDKTDRTIHCKNNEPEVCAKKFPKFIKREFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
BASE COUNT         1328 a          445 c          494 g         1130 t
ORIGIN      
        1 aataactacc tatggaaatg acattataaa tagattttct cttaactaca cataacaaaa
       61 cataacaaaa cattataaaa cataacaaaa acttagacat cgttttctta gtatgatata
      121 ctaaaaaaga atttacacta atttactaag ggggaatatc ttatgtttaa aaaactttca
      181 gtactagctt tagctatgat tatttcatta agtattactg catgtaattc taaaaaaaac
      241 aaggctaatg atgaatctaa tactaaagtg acaacagctg atgattcaaa agaaaaagag
      301 actctttttg tctattcagg ggcaggttta aagaaaccaa tggatgaaat agcacaaaaa
      361 tttgagaaag aaaataatgt caaagttgaa tatagctatg caggttctgc acagctaata
      421 gctcaaattg aaacaagtca caaaggagac gtatttattg ttggttctga accaatttat
      481 tcaaaggcct ttgaaaaaga acttgtaggt gaacataaaa cggttgcaca tcatacacct
      541 gcaatagttg tacctaaagg aaatccagct aatattcaaa aattggaaga cttaaataaa
      601 gatggagtta aacttatttt gggagacaaa gaatctaatg ctataggcaa gactacacaa
      661 aaaatacttg aaaaaaataa tttaaaatct ataaatgata atgttgtctc tacagctgca
      721 actgtaaatg aaatgattgt acagttaaca tcaagtaaag ctgatgctac aatagccaca
      781 aaagattcgg tctttggcaa tgaagatgta gaagttatag aaattgccca agataaaaat
      841 atagaccaac tcatatcagc aggaatcgta aactatagtg ataaaaaaga tttagctaat
      901 aaatttataa attatataga gtcagacgaa gctaaacaga tatttaaaaa atatggtttt
      961 gaacctgtta agtagtgatt aaacttgaaa aataaatttt ttactatact acttactgga
     1021 ttttttgcta tagtcttttt gtttattgta tcaccacttc ttatgttaat cattaaaggg
     1081 ataagttatg ttcctatatg cttaaaatca gtggaagtac agtatgcaat aacacttagt
     1141 attaaaacat ctttaatatc tactatagta tgccttttat tagctattcc agtagcatat
     1201 tttttacata ttactaaatt accatttaaa aaactgatta tacagataat aaatctccct
     1261 atgtctcttc cccacttagt atcaggaatt gctcttttac ttttgtttgg aagaatgggg
     1321 attggagatt ccatttacaa gattcttaag cttgattttg tatttacaaa gcaaggtata
     1381 attttagcac aagtatttgt aaatcttcca cttactatta aaatacttca cacttcacta
     1441 aatgaaagta atgaaaagat gatatttgtt gcacgtaccc ttggatgtaa tagttgggaa
     1501 gcatttagat ttataattct tccaaatcta aaaactggaa tcatatctgc aactgtaatg
     1561 acttggtcaa gagcacttgg tgaatttggt gcagtagcta tgatagcagg ttctacacgt
     1621 atgaaaactg aaattattcc tacatctata tatttaaata tgtctactgg tgatatagac
     1681 attgcaatcg gaatagctgt aattttgata tttatctcac ttacatgctt gatgcttttt
     1741 gagatatttt ttaatagagg agttgataaa aattaatatg ttaaaaataa aaaatttatc
     1801 tcttgattta cctaatttta aattacaaga aaatagttta aatattaata atcatgaata
     1861 ctttgtttta ttaggagcta gtggctcagg taaaactcta tttctagaaa cattagcagg
     1921 aagatatcca tctgctaaag gaaatgtatc atacaaagaa aatttaatat caaacctacc
     1981 tccagaaaat agaaatatag gatttgtata tcaaaatttt gaattatttc caaatatgaa
     2041 tgttgaagat aatataaggt ttccacttaa attaagaaaa atacctatag atgaacagat
     2101 tcttaaggct aaccacttat ctaaactaat gcatatagaa gatataaaaa gtagatatcc
     2161 taaaaattta agtggaggtg aaaagcaacg agttgctttt gcacgtgcct taatagctga
     2221 acctgatata ttacttttag atgaacctat gagttcattg gattatatta caaaaaaaca
     2281 tataagtaaa attttgaagg atgtctacat aaaatacaaa ccaacagtga ttcatgttac
     2341 tcatgatatt tctgaagcaa tgttttttgc tcataagata ggaataatga acaatggaaa
     2401 aataaatcat atttttgatt taaatgaaga aataaaaaat aaaggagaat cttttttcta
     2461 tgaatatatt tgatattttg agagattatc tattagtaca agtcgataaa tataatttaa
     2521 atatggatat gataagtatt gtatccaaat ctctatcttc aaaagaagca attggaaata
     2581 ctaagcgtaa agatttccca attatagttg gtaaagaaat aatgcttgaa gctgacttta
     2641 aaggagctaa aggacaagca tttacttcta ctccatctac atttgaaggt tctttaaaag
     2701 acatattatc attagattta tatgacaatc ctcatgatag aagtcttttt attgcatcat
     2761 taaatgcagt tatgaaatac ttagacaaga cggatagaac cattcattgt aaaaataacg
     2821 aaccagaggt atgtgcaaaa aaatttccta agtttataaa aagggaattt ggtaatccaa
     2881 aagtagcaat tataggatat caaccagcta taattgataa tattaaggac tttttcgaaa
     2941 caagagtatt agatttaaac cctgaatttg tggatactat tcaatataat gttaagattg
     3001 aagatggtat acgagattat gaagatgtaa tatcttgggc tgatttagtg atttgcacag
     3061 gaagtacatt atgtaataac tcaattataa attttctatc acttaataaa ccagtttact
     3121 attatggcac tacgatagct ggtgcatcaa atattttagg tttaaaaaga ctctgttttt
     3181 gtagtaaata agcaatcaca tcatcaagga gtacacaaat atatatgtgt actctttaaa
     3241 attaaaaagt tattactttt attctaagta ctaaagtaaa attctattaa aatgaatccc
     3301 gaataaatgt acaatccctt taaatctcaa ttcctatcaa tcattaatat tttgattagt
     3361 cttatacaca aatattttct ttattttaaa ctaataa
//
LOCUS       DAEMAE010000295         3397 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEMAE010000295 DAEMAE010000000
VERSION     DAEMAE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.299"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..1010
                     /locus_tag="KPH93_003471"
     CDS             84..1010
                     /locus_tag="KPH93_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG1690241.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMGGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(1130..2128)
                     /locus_tag="KPH93_003472"
     CDS             complement(1130..2128)
                     /locus_tag="KPH93_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG1690242.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYRFIDSVDKDMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            2232..2669
                     /locus_tag="KPH93_003473"
     CDS             2232..2669
                     /locus_tag="KPH93_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1690243.1"
                     /translation="MEDKYIVHFISKTKANMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            2755..3342
                     /locus_tag="KPH93_003474"
     CDS             2755..3342
                     /locus_tag="KPH93_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG1690244.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
BASE COUNT         1265 a          506 c          507 g         1119 t
ORIGIN      
        1 aatataaata tcttaattct caaataacat ctcatgtaat ctattcaatg caaaaaattt
       61 aaatattttt taggaggttt tttatgagca aggaaaataa aaatgtaaga attgctgttg
      121 tccaggcgtc accagtcata atggatttag aaaaaactgt tgaaaaagca ttaggattaa
      181 ttaaagaagc tggtagaaaa ggggcaaata tcgtagtttt tcctgaagca ttcatacctg
      241 catatccaag aggtctttca tttggatttg ttgttggaag cagaacaatg gggggtagag
      301 aagattggaa aagatactat gataattctg tgccagttcc tagtgctaca acagatttat
      361 taggaaaagc agcacaagaa gcaggagtat atctatcaat gggtataaca gaaagagatg
      421 gtaatgatat aaattgtacc ctttattgca caaacttatt tttctctcca gaaggtaagc
      481 taattggtaa acacagaaaa cttaaaccta caggaacaga aagatgtatt tggggagaag
      541 gagatggaag tactcttaca gtagttgata ctccatatgg aaaaatgggt tcgttgattt
      601 gttgggaaaa ttatatgcca ttagctagaa cagctcttta tgctaagggt gtaaaacttt
      661 acatagcccc tactgcagat tcaagagaag agtggcaagc aactatgaag catattgctt
      721 tagaaggaag atgttttgta attggatgca atcaatatgt tgaaaaaaat atgtatccaa
      781 ctgaccttaa ttactataag gagctagatg cagaaccaga gattatgtgt ccaggaggaa
      841 gttgtatagt tgaccctttt ggaaaatatg tagcaggtcc tattttcaat aaagaagaaa
      901 tgctaatagc tgatttagac ctagaaaaaa tagtacttag tagactagat tttgattcag
      961 aaggtcacta ttcaagacca gatgtatttg aattaattgt tcatgaataa tttttttaca
     1021 cagacatcta cataaatttt tttacataaa aaaatgagat actcataagt aaatttcaag
     1081 tatctcattt ttgcttaaaa cctagaatcc taattataaa tttattcacc tatatttttt
     1141 ctaaatcaac tactgttctt ccaacatgtt ttcctgccat tattgaatct atactatcat
     1201 ttaactcttc caatgatact acatttacca tatcatctaa atcatctaat ttccattcat
     1261 ttgataataa atcccatatt tttaatctta agttcattgg gcaatccaca gaatctatac
     1321 catataaagt aattcccttt aagataaatg gatatactga acttgtaaaa tcaactcctc
     1381 ctgcattacc acaagttgtt actgctccac aataattagt tgtctttatt gctgttgcta
     1441 gagtatttcc tcctactgta tctattactc cagcccatct tggtctaagc agaggtcttc
     1501 ctgaattatc atccacttct gacctatgta taacatcttt aacacctaag tttagtaata
     1561 cttctttttg ttcttctaat tttcctgttg aaactacaac tgaataacct aatttaatca
     1621 atattttagt tgcaattaca gcaactcctc cagtaccacc agtgactaat acatctccca
     1681 tatccttgtc tacagaatct ataaatctgt acacagataa agctgctgta aatcctgctg
     1741 taccataatc cattgcttct tttagtgaaa gatttttagg aagtttaact acccaactag
     1801 aaggaactct tatgtattct gaaaaaccac cagaagtatt cattcccaag tcataacctg
     1861 ttactaaaac ttcttctcca attttaaaat catcacaact actagattct actataccag
     1921 atgcatcaat ccctggtgta tgaggatact ttctcgtaac acccttatta ccatttgcag
     1981 aaagtgcatc cttgtaattt agagaagaat attttacttt tattagaact tctccttctg
     2041 gcaatgtatc tacttctctt tcaactatat ttcttctaaa attgccttct tcttctgtaa
     2101 tatatagtga tttaaatttt ttattcataa atttcctccc aatacattta gttttatgtt
     2161 aaactaatta tagtttataa atagttttat gtcaaactaa tttaaataga ttaaagattg
     2221 gagaataatt tatggaagat aaatatattg tacatttcat aagtaaaaca aaggctaata
     2281 tgattaaatt cattgaaaat aaactcagta aaaatggttt aggtgagtta atacctactc
     2341 atggaaatat tttaactgca ctttatgaaa ataatggaat acttacaatg aaagaaattg
     2401 cagctaaaat aggaaaagat aaatccactg ttacagtgtt ggttaataaa ctcataaatc
     2461 taggatattt agaaagacaa aagtgtacaa atgacaaacg tatcacgtat ataaagctta
     2521 caaaaaaggc attacttata gaagatacat ttaatagcat ctcaacacaa gttaaagaga
     2581 cagcatacca taatattaca gaaaatgaaa aacaagaatt tcttagaatt ttaaaaaaaa
     2641 ttaatgataa ttttaaaaat gctgattaaa ttgagcataa ttatatctcc tattggaata
     2701 ataaataaaa agaatcaaat attgtaaaaa tttattgtga tagagaggta gaatatgaat
     2761 ttaaaagata gtaaaactaa agaaaactta ttaagagctt ttgcaggtga gagtcaagct
     2821 agaaatagat acaatatttc tgcatctgtg gcaaaaaaag aaggattata tataatagag
     2881 tctttattta attacactgc tgaccaagaa aaatcacatg caaaacaatt cttttctaaa
     2941 ttaaaagaat ttgctggtga aaatataagt atatcaggag cataccctgt agatttatat
     3001 gataaaacag cacaacacct taaagctgca caacataatg agcttcaaga gtgggatgat
     3061 gtatataaaa actttggaaa aacagcacgt gaagaaggat ttgaatctat agctaagtta
     3121 tttgaaaata tagctgaaat agaaaaagtt catggtgata gatttggtag atatgctact
     3181 gatttagaag aaggtacttt attcagaaga aatgaagata tacaatggat atgtacacat
     3241 tgtggccata tacatatagg taaagaagct ccaaaagctt gtcctgtatg tcttcatcca
     3301 caaggatatt tcttagattt taaaaattca ttatttgaat aaactaaggt aatcattgta
     3361 tttaaataat ttactatatt tatataagta tttttat
//
LOCUS       DAEMAE010000296         3349 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEMAE010000296 DAEMAE010000000
VERSION     DAEMAE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.300"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..1387
                     /locus_tag="KPH93_003475"
     CDS             278..1387
                     /locus_tag="KPH93_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1690245.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1765..2949)
                     /locus_tag="KPH93_003476"
     CDS             complement(1765..2949)
                     /locus_tag="KPH93_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690246.1"
                     /translation="MQKNVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWKPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1275 a          482 c          425 g         1167 t
ORIGIN      
        1 tttctttttt gtgaaaactt cttaaaaatg aaagttatta tgtttttctt gcattttaat
       61 ttaattttta aatagattta ttttcaaaaa acctttaaaa ataaacctat aaaattccta
      121 ctaaaattta atttaagtta cattccattt taaagtatct taatatattt ttaagctata
      181 taaataataa ttaaaaaatt tactaaaaat acttacaaat atgatattat taattgatta
      241 ataaaaaata aaatttttca tgaaaggtaa gataacaatg gatttcaaaa gcttagggat
      301 tagtgaaaat acaataaata ttctaaaaaa atcaggaata acaacaccta cacctataca
      361 aaaagaaagt attaaactaa taaaagaagg aaaagatgta atagcagaag ctcaaactgg
      421 aacaggaaaa acactagctt ttttacttcc gatatttgaa aatatatcat tagatataaa
      481 tgatatacaa gtacttatac tttctcctac aagagaacta gctattcaaa taacagaaga
      541 agctatgaaa cttaaagaat ctaaagatgt aagtattcta gcagcatatg gaggaaaaga
      601 tattggttct caaataaaga aactaaaagg aaatattcat atgattatag ctacaccagg
      661 taggctttta gaccatctaa atagaaaaac aatagacctt agtaagttaa agacttttgt
      721 tttagatgaa gctgaccaaa tgttgcttat gggatttaaa aatgaagtgg aagctatttt
      781 aaaagagact tcaaacaaaa agcaaacact atgcttttct gcaactataa attctcaagt
      841 aaaaaaatta gcttatagat acacaaaaaa tcctgttgta gtttctattc aaaaagaaga
      901 aatcactctt aacaatataa aacaagaagt agtagaaact acagatagaa aaaaattaga
      961 tgccttatgt aaggtgctag atgaagataa tccttttatg gcaataatat tttgtaggac
     1021 taagagaagg gttgacaatc ttgaagaagc tcttgctata agaggatata attgtcaaaa
     1081 acttcatagt gatattgctc aatcaaaacg agaaagaata atgaaatcat ttagaaattt
     1141 agatatacaa tacttaatag ctacagatgt agcttctcgt ggtcttgata tatctggagt
     1201 aagtcatata tataattacg atttaccaga aacaccagaa gactacatcc accgtattgg
     1261 tagaactggt agagctggtg aagaaggcta tacatgtgct tttattgacc ctaaaaatga
     1321 acgaatgtta agtgaaatag agacggctat agaatctaaa attagtagaa gaattataga
     1381 attataattt atagactact taaaatacca atgtcacaca aacactgtaa tatatagaaa
     1441 gctacagaaa acatgtatca atctattctt tgctgttata ctattttata ttaaagaaaa
     1501 aagaggctat ctaaaagttg gatatgtctc ttttattgtc gaaataatga aaatatattt
     1561 gacttcatat aaaaaagagt gccttataaa ctaaaagtcc attttaagac accctctttt
     1621 tatcttttat tcttagtttt atattgcttg tttatagttg tttaatatta gattttgcaa
     1681 ataatcttaa taaacatgca catatattat tcatagtatt cttatttaca gtattctctt
     1741 tagcaatatt ttttgctact acatctaatt tacattttca tttaattccg ctggccattt
     1801 agcctctgga ttttcctttg caaacttctt attcttataa tgtcctagtg taaggaatgg
     1861 aattactata gtgaatagac caagataacc acaatatcca tatccatatt taacaatatt
     1921 acttaatcct gtaagtgata taagcattga cactaccatt attgcacaag ctgtaaatac
     1981 ccttcttttc attcttgaag aaaggaaaga aagtgcttta tggtcttcaa atctatttac
     2041 aagaccaaat atagtagtta ctcctgtaga cattaaacaa aggaataatg caacattata
     2101 aattatgtat atagtatgat ttccagattg acctgcaaca taaagagtag gaagtgttgt
     2161 ttgcccagca gcaataattt ctctatacca tcccattagc atagttacag caagccctaa
     2221 tgcaattgca ttcataacaa atgaaattat cattgcctta gtagcttctt ttgagttctt
     2281 aagaagtggt cttgaacatc ctgcaagtgt aggtataaca accgattgaa aacctgcata
     2341 aataaaagta ttaagtattg gcttgatata gccaggtgtg aaactcgttc tatttgataa
     2401 tatttcagtt atttctggta ctttagcttt tataccaaca ataaaaatag taagagtaca
     2461 tactagaata gctattgtca ttgctgtagc tgctttagat acaagcatag caccaaatat
     2521 agtaaagaca agaagtacaa ctccaataat aaatacagct acaaaataat ttacccctat
     2581 actttgaaat actgctgctg cacctgctat aacagcactt acagctatta acaccattaa
     2641 atagtaatat atctcccata ttatttcaag ttttggatat ggtttccaaa gctcacaaaa
     2701 taaatcctta taattcctac aattattatt attatacatt atgataactt ctctcatggt
     2761 agcagtaagt ataatcattg ctaaaattgc cataagtggt gctgtccatc catactgcac
     2821 ataatactga gtttcttgat tccctgtagc aaatcctcct cctgcatgtg aactaaaaag
     2881 aacgcttgca acaccaaaaa tggctaacat agagcctgtt acttttgctt tctttacatt
     2941 tttttgcatg aaatcatcct ccctaaaatg atattgaaag tttttatttt aacactttat
     3001 tgataaaaca ataacatttt tgttcattaa attcaaccat atttttccaa tagttttaca
     3061 ttttcaactt atttctactc ttatattaaa tattttttat atatttattt tattttttaa
     3121 atagtattca aattcacttt gttttttaat tttatgaaat atctttttta atgttaaata
     3181 ttatacattt tttctttatt attatacgat tttttcttta taattttata ataataattg
     3241 tatagtataa aatcccttct tctattgcat tcgttggaat tatcaaacct taaaaggata
     3301 aatcaatata ttttgtgaaa aaatatactt taatgtaaat aactttgat
//
LOCUS       DAEMAE010000297         3322 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEMAE010000297 DAEMAE010000000
VERSION     DAEMAE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.301"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..1282
                     /locus_tag="KPH93_003477"
     CDS             62..1282
                     /locus_tag="KPH93_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1690247.1"
                     /translation="MQQDLKLKYQTKELEKDFVIESCNSIKPWLINVRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKENADKTICIRADMDALPIEEEN
                     DIPYKSIYSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKLLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKICVKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKISKIVEDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRANPSLGGEDFSFYTEHCKGAFFHLGCKNDEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1319..2668
                     /locus_tag="KPH93_003478"
     CDS             1319..2668
                     /locus_tag="KPH93_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1690248.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAATLVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFANIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQVVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(2751..3218)
                     /locus_tag="KPH93_003479"
     CDS             complement(2751..3218)
                     /locus_tag="KPH93_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1690249.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT         1246 a          466 c          505 g         1105 t
ORIGIN      
        1 tatggcataa taattgctta ttatattaac caaacaataa aaagcaattg gaggaataaa
       61 aatgcaacag gatttgaaat taaaatatca aaccaaagaa ttagaaaaag attttgtaat
      121 agaaagctgt aattctataa aaccttggct tataaatgta agaagggaat tacataaaat
      181 tccagaatta gcccttgaag aaaatcttac taaacagaag gtcatttctt atttaaaaga
      241 aataggaatt gattatatgg aatttacaaa acacaatggg attatggcat atattttaaa
      301 agaaaatgca gataaaacta tttgtataag agcagatatg gatgcccttc ctattgaaga
      361 agaaaacgat ataccttaca aatcaatata ttctggaaaa atgcacgctt gtggccatga
      421 tgcacataca accatgttac ttggagcatg taaagtttta cattcaataa aagataaact
      481 taatgtaaat gtaaagttat tatttcaacc agcggaagaa ggttttggag gagcaaaatt
      541 cttagttgaa gatggatgtt tagaaaatcc taaggtcgat tatatattcg gacttcatgt
      601 tatgccacat atcgaaacag gatttatcga aacaaaatat gatacattaa atgctagtgt
      661 cgatactata aaaatttgtg tcaaaggtaa aagagctcat ggtgcatatc ctgaaaatgg
      721 tattgatgca atagttacag cttcacaaat tgttacatca ttacaaacta taataagtag
      781 aaacttagaa ccaaataatg ctgctgtttt gactataggt aaaatatatg gtggtgatgc
      841 acacaatgta atatgtgaag atgtaaaatt agaaggcacc ttaagaacct taaatagcaa
      901 aacaagaaac tttatgattg ataagatatc taaaattgtt gaggatactg catctgcatt
      961 tggctgtgtt ggaactttgc atgtaagtga tgaaaattat cctgctgtta taaatgaaaa
     1021 agaattagta gacacagtta tatcaagtac taaagaatta cttggagagg aaaaatttat
     1081 attgagagca aacccttctt tgggtggtga agatttttct ttttacacag aacattgcaa
     1141 aggggcgttc ttccatttag gatgtaaaaa tgatgaaaag ggattaattt cacctcttca
     1201 tacctctagc tttaatatag atgaagattg tttaccaata ggtgttatga tgcatgtaat
     1261 gaatactttg tattttaatt agtacagtaa ttaataaaag aataggagat aaattaatat
     1321 ggaaaaagct aagaaattag gtctatttag tatgatatta ttagggatta atagcataat
     1381 aggttcaggt atattcttac taccaggtaa agtttataat ctagcaggtc aaaacagtat
     1441 gtttatatac atttttgcta cactcttagt tctatcaata cttttatgtt ttgcagaagt
     1501 tggaagcatg tttgataaga atggaggagc atatctatac tccaaaaaag cttttggaga
     1561 ttttatagga tttgaagttg gtacaatgtc ttgggttatt agaattatat catggtctac
     1621 tttagcagta ggatttgcaa cagctttagg ttcattttgg ccagaatctg ccactgaata
     1681 caagggctat atagcagcaa cactcgtaac actactttca ataaatagct tatttggaat
     1741 taaaagcaca aagataatga ataatgtaat aactatagct aaattagtac cattaatagt
     1801 ttttgtaata gttggaatat tctttataaa atttgcaaat attgttcctt caggaaatgt
     1861 agttaactct agtatgggtc cagccattat attagtattt tacgcattta ccggctttga
     1921 atcatttatt gtggcaagtg gagaaatgga aaatcctaaa aaaaatcttc caattgcatt
     1981 aataactaca atatttatat gtgcaataat ttatatatta attcaagttg tatgtatggg
     2041 tatacttgga gatagactat ttgaaaacag tattccaatt gcagatgctt caagtgtatt
     2101 tttaggcaat tatggtaagg ttttcatatc tgtagcaaca ttaatatcta tatttggtat
     2161 aaatataggt tcatcaatag taactcctaa atgtggttct tctctagcag aagaaggttc
     2221 tcttcctgca tttataggaa aaacaaataa atatggtgct ccatatgtag caataataat
     2281 atctttaatt tgttgtatac cacttgtgct aactggaagt tttgaacaac ttgcagtaat
     2341 gagcgtaatt gcaagatttg ctcagtacat accaacttgt ttatcagtaa tagttttaag
     2401 aaaaagaaca gatgttaagg cctcttttaa gattccattt ggtcctgtta taccaatagt
     2461 agctatttta ggtagcttat ggttgttaca acaagcttgg gcagaagata tttcaaaacc
     2521 tataactcaa aatagagttt taataggatt aggggcaatg ttgttaatag ctcctctata
     2581 tatatttatg aaaaaaaata aggaaatcga gcataaaaat aaatcagaag attctaaatt
     2641 atatatttcg agagaaaaaa ttcaataata ttttacaaac tatttattaa aaataataaa
     2701 taaaggcaaa agcacagatt agctgacgcc tttattttaa tataatccat ctattctaaa
     2761 actgattcaa tatattcgac agttacatcc attcttttag ctacctcatc tattgaaaga
     2821 ccactatcta aaaaacgtct acacacagat gacatagttt ctccaacctc aataatatgc
     2881 ccatctaaat cataaaatct aattgctctt tgaccccacc tatgctctat agctagatga
     2941 acataatcta tatctctcat tgtactcaat ctttcaacaa aagtatcaaa ataatcttcc
     3001 tcaaaataca gctcagcatc atttccattg aacttaactt cactgtcact cttattaata
     3061 aactctaacc aaagcttttt ttcttgtaat gatatattcc cttctaatac aacattttct
     3121 ccaaaatcac agattacctt taaccccaaa acatcataat aaaacttttt tgatttctca
     3181 atatcttcaa ctactattaa aacatttaaa aacttcatat tacctcctta atatacacat
     3241 actttattta tattatataa ttagaattaa actataaagg tttcaataag gtctagtata
     3301 aagaaaaaaa ttataagtct ta
//
LOCUS       DAEMAE010000298         3305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEMAE010000298 DAEMAE010000000
VERSION     DAEMAE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.302"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(73..516)
                     /locus_tag="KPH93_003480"
     CDS             complement(73..516)
                     /locus_tag="KPH93_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690250.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(519..716)
                     /locus_tag="KPH93_003481"
     CDS             complement(519..716)
                     /locus_tag="KPH93_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690251.1"
                     /translation="MKNRLEEIRKERGIKQEELASVLQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFNMNIEEIFIYEEE"
     gene            complement(908..1774)
                     /locus_tag="KPH93_003482"
     CDS             complement(908..1774)
                     /locus_tag="KPH93_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1690252.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINSPDY
                     TTEVWIPIQKPS"
     gene            complement(2000..2869)
                     /locus_tag="KPH93_003483"
     CDS             complement(2000..2869)
                     /locus_tag="KPH93_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1690253.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENASNAKVLNATKTGQNYSK
                     SDMQVVIDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
BASE COUNT         1129 a          583 c          327 g         1266 t
ORIGIN      
        1 tatattctaa aactgtctta agaatctaaa gtttaaaaat ccaatatttt tattgtgaat
       61 ttaaactttt tattacaact ttttatctaa tttattgtat acaacaacac tacaaataaa
      121 ttgaaataaa ataagcaatc caagaacaac taaaacccac ttgcttatta ccattacaga
      181 aaatacactc aatatagaaa caataaatat aagaattaca agaacatagg acatgatttg
      241 attagtgata cgtccagctt tatcacgtaa cataactctt ctttcatcat tcatctctat
      301 cctttggttt tttatctttt cttcataatc tactgctctt tcaggcttac tccaatacat
      361 atattgaaga atcatacata tacctggacc taaagcagct ccagataatc cccaaataaa
      421 agcctcaaac gaaaactcag taaacgtagc caataaaaca agtaaaattc cacaaataaa
      481 atatactatt ccaagatata acatattctt tttcattttt actcctcctc ataaataaat
      541 atttcttcaa tattcatatt aaagtatcta gctatcttaa aggctaagat gatagaagga
      601 ttatacctac cattttctaa tgaacctata gtttgtcttg atacttgcaa aacagaggca
      661 agttcttctt gttttatgcc tctttctttt cttatttctt ctaatctatt tttcacatct
      721 atcccccttt aatatctcaa ctatggaaag tctactttcc ataattatac aactatattc
      781 catttatgta aagcttactt tccatattta caaaaaaata acagactatt ttcagcctgt
      841 aattttaaaa tttcatgttg tttattaaat ttaatgttat ttattaattc catattattt
      901 attaacttta acttggtttc tgaattggaa tccaaacttc tgtagtataa tcaggagaat
      961 tgatatcgcc attgggataa agttcaatat ctggtaaatt agcatacaca tacccagaac
     1021 ttggtaacca ctcagtaata attctttttt gcaactcctg tattgccaga gcatttggca
     1081 ttggagaaac acattcaaat actgcccatg tacctgatgg tattaagtat tcatgagtat
     1141 cttctaatac aggcttatct gttggtgcag caatataata atctaagccc ttactatttg
     1201 tacacacact aactccaagc aatccacaag gttcgttatt tattaactct gaaagtttat
     1261 caattgttcc attttttatg catttagccc ataacttagg aactttctca aaacactctt
     1321 ctacaatcat gttcatactt tcttttatac caacaatacg aaatcctttc ttttctacaa
     1381 tacgatattg catttctaca tttcctttta cataaatgct taatgtcatc ttaggatatg
     1441 cttttaaaac aacacccttt gctctagctg cagatggaga aatgttatgt atattttgaa
     1501 aagctctatt aaaagatgtt ggtgaatcgt aaccatactt ctcagatagt tctaaaattt
     1561 tgatgttact gttctgaagt tcaaatgcag ccttagtcat acgtcttcgt ctaatatatt
     1621 cagataatgg tacttctgca atatatgaaa acattctttg aaaatgaaag gttgaacaac
     1681 atgcaatttt tgctgcttct gcataatcca ctccactttc gagattttct tcaatataag
     1741 taatagcttg atttaattta tttatccatt ccatttgata ttacctccaa gcaaattata
     1801 cagtttaata aaataatata cctctctatt catgcacaaa attgtaagca tttaaattca
     1861 taagtatttt tcaatcaata caataaatat aaaaattgta ttatacaata aaaaagttca
     1921 ataacattat attataaaat gttattgaac tttttataaa aattaattta agtgtagtta
     1981 aattaagtat tacactatct tatatattac aatatcctac tatcttatag tcttctggca
     2041 ctttataatc tttgtctgga gtacctaaat tccattttaa atcaaacata gttgcactta
     2101 ttattgttgc aaaataaagc ataactatac caatgtctat tttttcttca tataaattag
     2161 tgtgaccatc ttttctaaca gctaaaacta cagtgcctcc atcaactata aatctccatg
     2221 gttgtctatt taatgtagat ggagctaatc ttgcatagtg aaatgcatca agaagagctc
     2281 tttcttctaa gatttctata cttgcaacat ttccccattc gttcatataa acaacatctt
     2341 ctacacctag tctagtagac cccttatcta ttacaacttg catatcagat tttgaataat
     2401 tttgcccagt ttttgtagca tttaatactt tagcattact tgcattttct ccatatccaa
     2461 tagctataat tcctgttacc tctttatctg aattaattcc aagtttttct tttacctttt
     2521 ttccatcttc aaaagtaacc caacaagaac caattccaag ttctgttact ttaaagatta
     2581 tactttctcc tatgtaccct gaattttcaa tgtaattatc ttttatgtca gataatatta
     2641 taatataact tggagaatct atcatatttc ctttgtaccc tgctacttta tctaaattat
     2701 taaaaacttc acttctattc ataattctta catcaacatc tatttctgga actagtttct
     2761 tacaagattc tgagtatttc ttaatttcac taagatattc ttctttaata tctgtgttct
     2821 tatattctct aacagacttt ctatttaata tgattgtttt ataatccata attttatcct
     2881 ccccaaacta gtattgtttt aattatatac ccattaatat attattttac tcatttattt
     2941 aataaataaa gattacaatt ttgtaattat tatttattaa taatattcca cttttacatt
     3001 aataatgacc agttgtgcaa aatccattgc caaccaccaa ataaaaacat attataaagg
     3061 taatataaaa taacaaacaa atcttaacaa aaaattaaac tttttttaca cgaaagaagg
     3121 cttcccgttg ccttctttat ttttataaaa aataagctgt ttctaaaatg atttcacatt
     3181 tttgaaacag ccaagttatt ctataatgac aactataatt tgaggatgtg agggagagtt
     3241 gtcattataa atttagggta attatattat gttttaagct agttctttct atatttatat
     3301 aatac
//
LOCUS       DAEMAE010000299         3298 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEMAE010000299 DAEMAE010000000
VERSION     DAEMAE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.303"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..781
                     /locus_tag="KPH93_003484"
     CDS             134..781
                     /locus_tag="KPH93_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG1690254.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(948..1517)
                     /locus_tag="KPH93_003485"
     CDS             complement(948..1517)
                     /locus_tag="KPH93_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690255.1"
                     /translation="MKKFIICIIMFAISVLVLAGCSNDNKIKNEVDSMFNAIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKTGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1522..2421)
                     /locus_tag="KPH93_003486"
     CDS             complement(1522..2421)
                     /locus_tag="KPH93_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1690256.1"
                     /translation="MKLLSKINNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKIPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAVVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSSDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2432..2581)
                     /locus_tag="KPH93_003487"
     CDS             complement(2432..2581)
                     /locus_tag="KPH93_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690257.1"
                     /translation="MSNFIKDNKKGISIIVISIIFIVLGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2574..3158)
                     /locus_tag="KPH93_003488"
     CDS             complement(2574..3158)
                     /locus_tag="KPH93_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG1690258.1"
                     /translation="MKKFFMVVLGCVFVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKTNE"
BASE COUNT         1160 a          560 c          368 g         1210 t
ORIGIN      
        1 ctatattgta actattttcg taatcttagg aaaagattac ttataaaatt aaccttatac
       61 aataatatat cgtataatct taattagcat aatagttaca atattaaaat ttaaatatgg
      121 gagtgatttt agtatgataa gacaaacacc attggaaaaa cgatatgaca agctaggtcc
      181 tcatattgta acagcactca aaaaacgtta ttttgatgct tattactgta aaactagaaa
      241 tgaagctttg caacaaattt tagatttaat tcctcaaaat catgtagtat catggggagg
      301 atctgaaaca cttaaagaaa tgggcgtaca aactgctgtt agagagaaag gatttaaagt
      361 tattgataga gatttagcaa aaactccaga agaaagaact gaaattatgc gtcaagcact
      421 tctatgcgat acttttttga tgagtagcaa tgcaattagt gaagatggac aactctttaa
      481 tattgatgga aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa atgttatagt
      541 tgtagctggt atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa gaactagaac
      601 aattgcatct ccagcagttg ttcagcgttt tcaagatgtc aatacaccat gctatattaa
      661 tggaagttgt atagattgta caagtcctga aagcatttgt gcatacatgg ttactacaag
      721 aatcagtcgt ccagcaaaaa aaataaaggt aatattggta ggagaaaatc ttggtctata
      781 aaatatacat acacgtaaat agctctataa ataataaaat catatgaaat aacttatttc
      841 taaaagtggt agaataaaag cttctaaaag actttcattt taccactttt atgttctttt
      901 aatttgtgct tctctattat taattgctcc gattttcata tatattacta actattttta
      961 ttttcataat ttttttgttc ttcatcaagc tgtttataat aatctttatt atagtcagct
     1021 agataatcta taccacctcc tgtaatcaca tttaataatt tcattgcaga ttcttgatta
     1081 actttacaaa cataattatc accagtttta acaagctcta atgtaatatt aaattctttt
     1141 ttctttgcag aatcaatatc tttcatagtt ggatttggca tcatttctcc tagtttagga
     1201 ggattctttt ttaggataga aaccatatca gtattattaa tttttaaatc tacagtcact
     1261 ttatccttat tattctttat atctgaaatt tcccaagtca tatttttaga atacatttta
     1321 aatccttctt tatcttcgta tataagatta cctaaccctg attttgggaa atacttatca
     1381 acagtatctg catcttgtgt ttttattgca ttaaacatac tatccacttc attttttatc
     1441 ttattatcat tactacaacc agctaacacc agtactgata ttgcaaacat tataatacat
     1501 ataataaatt tcttcattaa attatatccc cctatacttt tttgcacaaa tattattttc
     1561 ttttttatct tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt ttatacattc
     1621 accacaccta atacagtctg aactatttac atctttatat attggtatat caagcttaca
     1681 ttttgtttga catgctgtgc aattcgtaca tttatcttta tcaattttaa atctatatat
     1741 tgaaatagga ttaaaaaaac catagattgc tcctaaaggg catatatact tgcaaaatgg
     1801 cctataagta atgattgaca tcaataatat caatagcaaa atacttaact tccagctaaa
     1861 taaaaatcct attgattctc ttaaattctc atttcctaaa ataagtggta ttcctcccaa
     1921 aagtgttcct gaagggcaaa tccatttaca aaaccaagga tttccacctc ctgctacatt
     1981 tacaacagca actggcagaa taattacaaa cattattaag actatatact ttagatattt
     2041 tagatattta tcaccataaa ctttttttat ctttactgga aagggtattt tatgtagtaa
     2101 atcttgtaca agtccaaagg gacaaagcca accacaaaca aatctgccaa aaaaagcacc
     2161 aaatgccatt aaaaaaccaa caacataaaa tgagaattta tactctctgc ttgataaaac
     2221 tgcttgtaat gaacctattg gacatgctcc aagtgcacct ggacatgaat aacaattcaa
     2281 tccaggtaca cataattttt tactttctcc tccatatata gttccttcta aaaatccatt
     2341 tacatatcca tttgttaatg ctgtaaatgc aacttgagta ataagtctta tattattatt
     2401 tattttagat aataacttca ttcctcttct cctaaccaat accaatacac tcaagacaaa
     2461 tcataattgc tttagtcaat actgtatttg tttctccacg gtatgtacct aatacaataa
     2521 atataatact aattacaata atagaaatgc cttttttatt atctttaata aagttactca
     2581 ttggttttta ccatttttag tctttcatct ataatttttt tccaatcttc cttactattt
     2641 gcacctacaa ccttctcacc cacaaagttt cctttcttat ctacaaaata agttgttgga
     2701 aactccataa ctcttttaaa atcagttttt ttaagttctt cagatacagt caattgttgg
     2761 taagttgcat ctgacttttt cataatgtct ttaactaaat ttttctcttc atttgaaaga
     2821 ccagttctca tatcagtttt atcaacttct acaactaatc cctttatagc tacattggaa
     2881 ttattactct catattcttt tgataatgct tcaagctctg gcatttcacc tacacatggt
     2941 ccacaccaag tagtccagac attaataaga gtaagttctt tatttgaaaa tatctctttt
     3001 gttaccttat ttccatcaac atcctccgta tctaaattac taaaccattc actattattc
     3061 ttgttttttt ctactggctt agattctttt ttactacatc cagaaatcat acctaaaaca
     3121 aaaacacatc ctaaaactac cataaaaaac tttttcatat ctcacactcc tagaatttat
     3181 atagtatata aaatcgctgc ttgaaataaa aataacttct cttctatatt aaaacattat
     3241 atttaataaa taagaactaa tttaaaataa gttgtataaa agatataaag ttaaaatc
//
LOCUS       DAEMAE010000300         3291 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEMAE010000300 DAEMAE010000000
VERSION     DAEMAE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.304"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..817)
                     /locus_tag="KPH93_003489"
     CDS             complement(<1..817)
                     /locus_tag="KPH93_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG1690259.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPINKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKT"
     gene            complement(1065..1739)
                     /locus_tag="KPH93_003490"
     CDS             complement(1065..1739)
                     /locus_tag="KPH93_003490"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG1690260.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            complement(1776..2147)
                     /locus_tag="KPH93_003491"
     CDS             complement(1776..2147)
                     /locus_tag="KPH93_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG1690261.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            2214..3269
                     /locus_tag="KPH93_003492"
     CDS             2214..3269
                     /locus_tag="KPH93_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG1690262.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVRESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKKNMKIDLGAIAKGYAADKIVAYLKSENVKKSLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTNKNGESIGNIETTNKSVVTSGIYERFIEKDGKMYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNK"
BASE COUNT         1087 a          527 c          410 g         1267 t
ORIGIN      
        1 tagtttttct tccccagtta taaactgttt ttgaatcatt catgtagata tcaattttat
       61 ttccttttat tgctccacca cagtcttctg cagtaaacac catgtcaaat tgaggtatat
      121 aaactttagt tccatatggt ataaccttag ggtctacagc tattgttccc cacttaggtt
      181 ttgttcctgt tgcagttata ctgtgaccag tatatgcagt tgctaccact ctcatatttt
      241 taccattgtt actactactg ctactagatg atgatgaagc cattcttctt gaactttctc
      301 cccttgatga ggctacttgc atttctacct ttgtcccttt gcttacaacc ttatttattg
      361 gctcagcttt aacaaccttt ttcacaaatt ccttttctac tttctttcca ttgtgataag
      421 tttctttata tattaattca cttttaccag acttaccttc ttctttaacc ttagaatcac
      481 cctttaataa agagccatct tcaacaactg taacttcaaa tggaatgtct ttagtttcta
      541 caactgtttt ttcctttaca tcaattactt ctattttatc tccattagat aactttgtat
      601 tttcatctaa agttactata tcatttgcat catagctaat gttttgagat tctaaaaggt
      661 ctcctacatt agaactaaat gttgatatag ttctttcttc tccccttact actaatgtta
      721 cttgtttatt aagcgcagag tatcccgata atattccaac tgataaaaca actgccatta
      781 ccgagacttt agcattactc aattttttaa aattcatata aaatttcctc cctaagattt
      841 tttacactat atattttgct tcataaaatt ctgctttgta agtttctttt ttatttgtgt
      901 tttttgtgta actaaattgt aactattttt tcaagagttg tcaacttgac aaaaaaatag
      961 aaatatgcat atatagtatt ttcaactatt taagcatatt tctattagtt acaacaatgt
     1021 cattgtttat tgttgttttg ttatcatttt gttacctttt acttttatat attttctatc
     1081 tgccaattaa taggttcttt tcctagcttt ttcaaaatct catttatctt agagaagtgt
     1141 ttacatccaa aaaagccttt acttgctgac agtgggcttg gatgaggtgc ttccaaaata
     1201 taatgtttac ttgtatcaat caactctact tttttacgtg cattacttcc ccatagcaca
     1261 aaaataactg ggtcttcacg ctcacttaaa atagatatta ctctatcagt gaaaatttcc
     1321 catcctttgt tcttatgaga attagcttca tgtgctctaa cagtaagagc agtatttaaa
     1381 agtaacactc cttggtctgc ccaaggcatt aaatatccat tgttaggtat aaaacaacct
     1441 aaatcatctt ttaattcttt atacatattt aatagagatg gtggagtttt cacttgtggt
     1501 tgaactgaaa aagctaaacc atgagcttga ttatctccat gataagggtc ttgtcctaat
     1561 attaagactt ttgtgtcttt gtatgaagtg tgttttaaag cttcatatat attgtgcata
     1621 tttgggaaaa tttgacgtgt tttatattct tctataagaa attttcttaa atttaagtag
     1681 taatcttttt caaattcttc tttcaataat tcatcccaat cgtttcctaa gttaaccata
     1741 attatcacca attcctttca attattttta ttattttatt tagaaattat atcttcttga
     1801 ttatcattct tttcatttgt atttattttt atgctcactc tattaggtat acaaactatg
     1861 ctttgacttg gcttgtttat aaatccagta tgtacacaaa ccttatcagg gcatgaagca
     1921 tctactatct caaccccttt atcatgaatc tttactatgt tatacccccc tctattctct
     1981 attttaaatt cttctttggc atttattgaa acagttttat acaatttatt atctacataa
     2041 atctcaatat ttccagcatt tttagaattt ataacattta ttatcccata acatgctgta
     2101 ataacaacca aaatgcctaa aataagaaga atgtcttttt tcttcatttt ttgtcctcct
     2161 atgaaattat gttataataa aataataaca caatttcata ggaggaatat accatgaata
     2221 agaaaaaaat cacatttatt tttgtgatat tgattttagc attaatttta ttttcatttt
     2281 ttaggagttc aagccagaat actaaatata gtaaaaccag ctactacctt ggtacagtaa
     2341 acgaagttac tgtatttaat gtaagagaat ccaaaagtga taaaatctta aatgaatgtg
     2401 attcaatttt aagatatatt gacaacaaaa tgagcactca tataccagga agtgatgttt
     2461 ctaaaataaa tgataatgct ggtaagaaat ttgtcagagt ttcagatgat acatttttcg
     2521 tcgtaaaaga agctatacaa tattccaaat tatcagatgg atattttgac ataacaattg
     2581 gtccattatc aaatttatgg gctattggaa cagataaagc taaagttcca agtgattctg
     2641 agatacaaaa acttttgcct cttattgatt ataagaacat aatattggat gaaaaaaata
     2701 aatctataaa attaaccaaa aaaaatatga aaattgattt aggtgccata gctaaaggat
     2761 atgctgctga taaaattgta gcatatttaa aatcagagaa cgtaaaaaaa agtttagtaa
     2821 acttaggagg aaatatattt actttagaag atggtaaaaa tgataaacct tttaaaatag
     2881 gtattcaaga cccaactaat aaaaatggcg aatccatagg aaacatagaa accacaaata
     2941 aatctgttgt tacttctgga atttatgaaa gatttatcga aaaagacgga aaaatgtatc
     3001 atcatatgtt aaatccattt acagggtatc cttttgaaaa caatttaagt agtgttacaa
     3061 taatttctga taaatctatt aattgtgatg ccctatccac ttctacattt ggacttggtc
     3121 ttgaaaaagg tatggattta attaataaaa ttgataatgt agatgctata ttcataacga
     3181 aggataagaa agtgtactta actaagggta taaaagataa ttttaaactt acagataaat
     3241 ctttttctat tgaaaaatta aataaataat aactatatct gttatgcata c
//
LOCUS       DAEMAE010000301         3275 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEMAE010000301 DAEMAE010000000
VERSION     DAEMAE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.305"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(199..1650)
                     /locus_tag="KPH93_003493"
     CDS             complement(199..1650)
                     /locus_tag="KPH93_003493"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG1690263.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVEHDNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKKEAYKSS
                     DVNILNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTMTIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNIIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQCKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNDDDFYCELEK
                     AATLSGERVWKMPIYDEFRDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGSTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(1666..2106)
                     /locus_tag="KPH93_003494"
     CDS             complement(1666..2106)
                     /locus_tag="KPH93_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1690264.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            complement(2354..2782)
                     /gene="ytfJ"
                     /locus_tag="KPH93_003495"
     CDS             complement(2354..2782)
                     /gene="ytfJ"
                     /locus_tag="KPH93_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG1690265.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(2736..>3275)
                     /locus_tag="KPH93_003496"
     CDS             complement(2736..>3275)
                     /locus_tag="KPH93_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG1690266.1"
                     /translation="INILIIADINNKDICLKLNIKYMFNLININRQLYPAENSKNNDK
                     KEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTENIGLTSSVYVLINTLY
                     GNLFNIIDAEKMYLNVNPDFTKDFVLGNIRIHIRPRIKALFNIIIMINKIMNKNKGNK
                     EGDSNESNRFDTESYGNNS"
BASE COUNT         1045 a          601 c          281 g         1348 t
ORIGIN      
        1 acaactgcta tttttacatt tatttttgtt actagtatta tgtacatacc tactctttta
       61 tttaatagag atacttttat acttgaaaaa aagaaaaaga gtaaataatt tacttaaata
      121 aaaaataaag ccacatagat aatcttaaac tacaaaaata tccacgtggc tttataaatt
      181 atatcttata attgtttttt agatttctgc caaatagtac aatgttctaa cgggttctcc
      241 tgtagccccc tttgctttat atccaattga ttttgatgat gtacttgtcc ctgctatatc
      301 catgtgtatc catggagtac taccaacaaa ttcaccaata aaagctcctg ctgttataca
      361 tccagcattt ttaccaccag tgtttttaag gtccgcttct tctcctttta tcatatctct
      421 aaattcatca taaattggca tcttccatac cctttcccct gataaagtgg ccgctttttc
      481 taattcacaa taaaaatcat catcatttgt tactatagga gttgcaacat tgcccaacaa
      541 tgttaaagca gcaccagtta atgtagctac atcaaccacc tttgttactt tttcattgtt
      601 tataatgtag tatattgcat cagctaatgt aagtctacct tccgcatccg tatttaaaac
      661 ttcaatagtt ttctttgcca tagaactaat tatatctcca ggtttgtatg aacctcctga
      721 taaagcattc tcacatgcag ccactactgc tataacattt ttctttaatt tacattgaga
      781 tatagcacac atagcaccta taacactcgc agcgccagcc atatctgtct tcatatctag
      841 catagaagca tttgatttta aagaatatcc accagtatca taagtcaatc ctttacctac
      901 cagacctaat atatctgtac tttctttatc tccaaagtat ctcattacta tgaatcttgg
      961 tttattaatt gaacctcttg aaacagcgaa aaaagcatcc attcccaatg cctttatttt
     1021 ttcttcttca taaacatcaa cattaaaccc aaactctgca ccaagtttaa ctgtttctat
     1081 tgctaatgtt tttggatata ttatgtttga tggttcatta actagatttc tagctattat
     1141 agttgaatta gctaatattt tagccttttc tatttgacac tcaacttctt tttcagtaaa
     1201 tatagtcata gtatcaactt cttttctttc aaaatcctta ctttttgtct tatacttatc
     1261 aaacttataa tttccaagta atatcccttc tacaaaagaa cctatttcat cactatttaa
     1321 attaagattc agtatattaa catcactact cttatatgct tcctttttca atatattttt
     1381 taatttttta cacaaattcc cgcctatgat tctaattttt tctctattca ataactctct
     1441 cttaccaaaa ccaacaagac aaacatttac caaattgtca tgttcaacca atgttacagt
     1501 atctatacta ttttctttag aattaaaatt tctgcttatc atatttattt tatcaaatgt
     1561 cgtagaaaca tcttctccct caaatagagc tactactgtt atagcatttt ctaaactttt
     1621 taatatttct ttagaatact ttatattcat aataaattct cctttttaaa ataattgttc
     1681 tattatattt ttagactctt ccaaagaagt aaataattta tctttattat ctatatctaa
     1741 gcaatccatt ttatcactta ttatagatac aaactttagt atttcattat ccaatatctt
     1801 ttctccattt ttagttattc cataaaaagt atttcgtcta tctctactat cttcttccct
     1861 atatacatac tcttgctcaa ccaatttatt aagtagtata cacaaactag aagtagatac
     1921 atgtaaatct ctacttagat ttttaagctc tattttatca tgttttctta tttttactaa
     1981 ggcaaagaac tgtctttctg taagaacctt atctttttta tatctttcgt tattaataat
     2041 ctttattaaa ttatgaaaat tattcatact ttctaacaac aaaatagata attctttatc
     2101 gctcataaca ttcacctcaa aactaatgat ttataactcc ctcttatatc tcaaatgcgt
     2161 ttatatatct ataatataat acttcttttc tttttataat tatactatat atcaacttta
     2221 ttatctattt aaattgtttt aaactaaaat cttattagat ataaatgttt aaagtacaca
     2281 acaaaatagt gggatttata ctaaccccac tactttattt aaaactatat acatatttaa
     2341 aaatatttta ttcttacata ttgtttttat cttgattctt attactttgt gatatatttt
     2401 gaattttctc caatactcta ggagttatag ataatatatt atccactaga ttgatattac
     2461 tgtctaaact cattattcta gtctctccac tttcaactac tacaaatgct actggttgta
     2521 cagatatagc ccctgcacta cctccagcaa aattcgtatc tgatttttca ttttttaatt
     2581 caatttccct atctttatca tcatatcctt ttgagtattc tcctcctcct ataccaaaac
     2641 caattgtaac ctttgatata ggtacaacca ctgtagtatc tgtttttata gggtctccta
     2701 taatagtatt tgcatctata gaacctttaa ttgtttcaag agttgtttcc ataagactct
     2761 gtatcgaacc tgttgctctc attactatca ccttccttat ttcctttatt cttattcata
     2821 attttattaa tcattattat aatattaaat aaagctttta ttcttggcct tatatgtatc
     2881 cttatatttc ctaaaacaaa atcttttgta aaatctggat ttacatttaa atacattttt
     2941 tctgcatcaa ttatattaaa taaatttcca tacaaagtat ttatcaatac atatacagaa
     3001 gatgttaagc caatattttc agttccaaaa ttaatatttg aataaagttc atgtattttt
     3061 atctttttta ataatctata aatgctcaaa aggtcatcag ctaataatat acttgaatct
     3121 atattattct tcatcccctc ttttttatca ttatttttac tattttcagc tggataaagt
     3181 tgtctattta tatttattaa attaaacatg tattttatat taagctttaa acaaatatct
     3241 ttgttgttta tatcagctat tattaagatg tttat
//
LOCUS       DAEMAE010000302         3258 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEMAE010000302 DAEMAE010000000
VERSION     DAEMAE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.306"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            20..946
                     /locus_tag="KPH93_003497"
     CDS             20..946
                     /locus_tag="KPH93_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG1690267.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGVIVFGNKDNSEDR
                     KTNIITEIFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(998..1888)
                     /locus_tag="KPH93_003498"
     CDS             complement(998..1888)
                     /locus_tag="KPH93_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1690268.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            2076..3107
                     /locus_tag="KPH93_003499"
     CDS             2076..3107
                     /locus_tag="KPH93_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG1690269.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
BASE COUNT         1247 a          349 c          511 g         1151 t
ORIGIN      
        1 atattaggga ggggtaagta tgaaaaatca attaaatgaa aatcataaaa aggttgtatt
       61 ttttggagta ggcgctgtag gagctacttt tgcagaacaa ttttttaact ctaaatatga
      121 ttttaaaatt ctttgtgata atgaaagaaa gaaaagatat ttagaagaag gatttataat
      181 aaatggaaaa aggtatgatt ttgattatgt aactaaagat gagtataagc aagaggctga
      241 ttttataatt ataggtttaa aatataataa tttaaaagaa aatataaaag aattagatgg
      301 attagttgga aaaaatacag ttataatgtc tctgctaaat ggagttgata gcgaagagat
      361 aataggagaa agatttggaa ttgaaaaaat ggtatattca tatgttacca atatagatgc
      421 aaagaaaatc aataataata ttatacacac tactaatggg gtaattgtat ttggtaacaa
      481 agataatagt gaagatagaa aaactaatat aataaccgaa atctttgatg atgtaaatat
      541 agaatatatt ttatcaaaag atattcaacg agatatgtgg tggaagtaca tggttaatat
      601 tggtgtaaat caaacttcag ctatacttgg tgcaccttat ggagtttttc agagttctga
      661 gcatttaaga gaattagcaa aatctgcaat gagggaagtt gttgctatag cacaagcaaa
      721 agacatatct cttacagaag atgatgtgga acattcatta catagaatac tagaacattc
      781 aaaagaagga agaacatcaa tgcttcaaga tgtggaagct catagaccta cagaagtaga
      841 tatgttttct aagaatatct gcaaacttgg aaaaaaatat aacataccta ctcctataaa
      901 tcagactttc ttttatatga taaaagtaat tgaaagtaga ttttaaaggg tttttgtaag
      961 aaatattgat aattattgat tttaaatttt attaatgtta tatactattt aagttactaa
     1021 atatttttaa ctcttcaatg aaaatagttg ctgcttttga taaagttata tcttttggaa
     1081 atatatatga gaaatgtctg ttgtagctgc ttccaagctc tatgacagac agctcattgt
     1141 ttaaaacagg taagcttgtc actagattag agactattgt aattcctaaa ttatttctaa
     1201 cagattcttt gacagcataa ttacttccaa aaaccatcat actttttgga ataatttctt
     1261 taacacttaa aaacatatct aaataatctc tggttccaga accatcctcc ctgactatcc
     1321 atttttggtt ttgaagttta tcaaaactaa aatcttttaa taaatgacta ttgtatggaa
     1381 gtgctaaaac catcttatct tcaaaaaaat attcttgaat aaatgaaggg gaagagcaag
     1441 tgccttcaat aagaccaata tctaaaatat aatccttaac atgagaagat acaattgatg
     1501 tattttttat aaaaacttct acatctatgt caggatactt tttagaaaat aatgctagaa
     1561 aatttggtag aatatactct ccaatagtta aacttgcacc aatctttaag tgaccagtta
     1621 tagcatctga aacttggctt acatcatgat aagtaatatt taaaagattt aatatttctt
     1681 tagctctttt gtacaagatt tgtccacttt ctgttataaa aatggttttt tgtttaatag
     1741 acctgtttat aagcacaact ttaaaataat tttctagatt ttttatgtgt ttacttacac
     1801 ttggttgtga tagatttaag tattccccag ctttagtaaa gtttttgtat tcaacaacag
     1861 ctacaaatgt ttttaattct tcaaacaatt ttttgtctcc tttatctaat gaaattttac
     1921 attttttata tactttctac agataattat actatatata ataaccatta aaaacattaa
     1981 tcattacgat attatatatt tattttactt atggttatta caattatata ctattgctat
     2041 gaagaaaact atattaatta taaggtaggt atattatgtt aaagaaaaaa agcagtttga
     2101 cattaaaaac tataagggaa atattaccag gactgtttgt atcagtatta gtaggttata
     2161 ttagtatatt tatttctatt ctaattccta aggtaggggc agcatcaatt tctatatttt
     2221 taggtatgtt tgtaggaaac ctatttttaa atcaaaaggt atttcaaaaa ggttataaat
     2281 tttcagagac agatttgtta tcatattcaa tagtactatt aggagcaaca ctcagtgtat
     2341 ctaccttaat agacttaaaa gtttcaggaa ttttatttat aatattgcaa atgacaataa
     2401 caataatagc tgcactttat ataggtaaaa aactaggatt tgaagaaaat tttagatttc
     2461 taatggcaag tggaaatgca gtatgtggtt cttctgctat aggagcaaca gttccagtag
     2521 ttaatgcgac agataaagaa aagggaattg cagtaactat tgtaaatgtt actggtatat
     2581 tccttatgtt tttacttcca ataatatctc aatttttata tagccatgaa cttgtaaaaa
     2641 cgtcagcaat gataggtggg actttgcaat caattggtca ggttgtagca agtggagcaa
     2701 ttgttggaga aaatgtaaaa gatttggcta ctatttttaa gatagtaaga gtaatatttc
     2761 tagttgttgt tgtattagta tttggacata tgaaaaatca gtctaatagg gaaatccttg
     2821 aagaagaaaa ggatgagata aaaaagaaaa aagtaactat accatggtat gtaattggat
     2881 tctttataac ttgtgcactt ttttcaatga atattatacc aaaagaagta tctgtgttgt
     2941 gcaaagaaat aagtaacaaa ctagaaataa tagctttagc agcaattgga ttaaaggtta
     3001 atgtaaaaga tttagtgaaa caagggaaag aagtttcttt atatggttta tttgtaggta
     3061 cagtacaagt tgtatcagct gtagttttaa taaaaatatt tatatagcat aaaaatagta
     3121 tttgtaatat taatttgatt aattaaaata atatgatact aataatttat aatataaata
     3181 aaaaatgaat ttcgataagt ttaagtgggt ataaacttat agaaattcat tttttattta
     3241 aggtaaaaat ttaaagta
//
LOCUS       DAEMAE010000303         3257 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEMAE010000303 DAEMAE010000000
VERSION     DAEMAE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.307"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..618)
                     /locus_tag="KPH93_003500"
     CDS             complement(46..618)
                     /locus_tag="KPH93_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1690270.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(647..913)
                     /locus_tag="KPH93_003501"
     CDS             complement(647..913)
                     /locus_tag="KPH93_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG1690271.1"
                     /translation="MKDSSRAELMRKIQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(913..1122)
                     /locus_tag="KPH93_003502"
     CDS             complement(913..1122)
                     /locus_tag="KPH93_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG1690272.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1391..2251
                     /locus_tag="KPH93_003503"
     CDS             1391..2251
                     /locus_tag="KPH93_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG1690273.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLEPNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2299..>3257
                     /locus_tag="KPH93_003504"
     CDS             2299..>3257
                     /locus_tag="KPH93_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG1690274.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEDLYPHIQELTHISSEELESAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMF"
BASE COUNT         1246 a          378 c          534 g         1099 t
ORIGIN      
        1 taaattataa attatcaaat aataatagtt atccaaataa aaattttaga acattttttg
       61 agattcttct aactcgtaag cgtccattaa agcttcacca aatctttgat agtgaattat
      121 ttctctttga cctaagaatt ttaatacatc tataacatca acatcatctg ttaggtttat
      181 aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat gtaaatcggt
      241 tacaggattt gacataactg ctataggtct tacatcaaat ggaacaccat ttgaatctgt
      301 tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag cttttaactc
      361 ttctgggctt gcatcagaag tcaactgata aaccatagta catataagct caacgtgagc
      421 tagttcttct gtaccaatat ctgttagaag tgcacgcata ttacctgtag gcattgtgta
      481 tctttggctt aaatatctca atgatgcagc aagttctcca tttggtccac caaattgagt
      541 tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt gttgtatagt
      601 tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa taattaaatt
      661 ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt ggagcatacc
      721 caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc gcaaattgat
      781 tacataatga attataagtg ttgatagcat ttttatcatc gggatgattg tctaaatata
      841 agttcatatc aacacatgca aaagatgttt cctgaatttt tctcataagt tcagctctag
      901 atgaatcttt cattatcttc tacctcctct atattttttt ggcttagcat ataattcttt
      961 gttgtacatt tcagattcta gtaagtttag ttctggaaat atagttccaa attttaatgc
     1021 atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc tagccaaaga
     1081 tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa cctccttgtt
     1141 gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa atcgttacta
     1201 ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg ttatttatgt
     1261 tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta aagtaaaaat
     1321 tatagtattt tgtgtatgaa ggtagacttt tttgtaaaat attattaaca taaatatacg
     1381 aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc cttttgagga
     1441 aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagaa aaaatttatg
     1501 gtctaagttt atgaaggcaa taagagatta taaattagtt gaagaagggg ataagatagc
     1561 tgtagcgatt tctggtggaa aagacagcat tcttatggct aaaatgtttc aagagttaaa
     1621 aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg gataccatgc
     1681 aaatataaga caattactta tagataattg tgagtatcta aatataccaa tacatttatt
     1741 tgattcaaga atatttgaga tagcagatga aatagcaaaa gattatccat gttatatgtg
     1801 tgcaagaatg aggagaggag cattatattc aaaagcagaa gaattagggt gtaacaaact
     1861 tgcattaggt catcattatg atgatgtaat agagacaaca atgcttaact tattgtgtgc
     1921 tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag gtataaaaat
     1981 tataagacca ctttattata taagagaaga acatattata agatttattc aaaatagtgg
     2041 tatatggcca ttaaattgtg catgtatggt tgccgctaaa aaaacaggta ataaaagata
     2101 tgagataaaa gacttaataa agagcttaga accaaatttt aaaaatgtag aaaagtctat
     2161 atttaaggcg gcagaaaatg taaatcttga ctcagttctt ggctggcaaa aagatgggga
     2221 gaaacattct ttcttagaga attttgaata aaaatagtta aaaagaatca ctataaacaa
     2281 ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa tatgccaaat
     2341 tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc agttatgtat
     2401 gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt aagtaatgag
     2461 gatttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt ggagagtgca
     2521 ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt ttctgatatt
     2581 aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac agatatgaga
     2641 agtgctaaaa aaaataacca tccaaggttt gtaaataata ttagagattt atttgtagat
     2701 ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc attgaaaaat
     2761 ttacttgaat ttgttaactt agagttcagt ggagatgccc atgacccatt aaatgatgca
     2821 tataatttat ttattttaga tgaggcaatc acaaataatg tagatataca aaatctttta
     2881 ataatacgtg acataataag accaccattt aacgatataa attctaattt agataattgt
     2941 tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat tgtagatttt
     3001 tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga tgtaaatata
     3061 caaaatttag agattatgaa agatataagt aaaaaaatgg atactataga taaattgaaa
     3121 gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgacat gaaagatgta
     3181 ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga gataaaaaat
     3241 ataataaaaa tgtttga
//
LOCUS       DAEMAE010000304         3199 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEMAE010000304 DAEMAE010000000
VERSION     DAEMAE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.309"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..1690
                     /locus_tag="KPH93_003505"
     CDS             35..1690
                     /locus_tag="KPH93_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690275.1"
                     /translation="MNLDFHYYGTYLAAKIAGYNDSDAKTIAYAAQYVDESSKNMILD
                     DVNFAFPTIQTNLEFEKYYADFTSLGYKWNLDSLNEIKKVWIPFHFLPGNLNNQVKYN
                     GVKESKGLTTNWKFKDGDDQKFRLMCLPNSETVSAIINDLIYFHSTEEYKLQFIGIRM
                     HVLADTWAHMYFIGKPEWYINDIKEFIAEESKYIEETKWTKAEEYRNSSWHSINFTGT
                     PDVGGYESISYLGHGRIGHLPDYGYLNYRYVPNWSCTDNKVKIQKNNQDNFFKAFCQM
                     VYALKCIKNGNDFSINQYDNLTAQQKTEVSKVIATRKDDQSEAWKKAIQILGYNSLEN
                     FDKNKWKNEFIKSTNKEKTDYYYFNLVAKKHVNYVIDFLEEKRLPLEEMCSKYCEFKT
                     FDDTQFAKNRNRVTKIILRGAYIVDAIQLVYDGKYKTPMHGETLGGSMVSLDLEADDY
                     IVKISGSMGLYEGGEPYPKSPTRTIGKITFLTKKGKTITTGCETMFKSYGNFTLEAPA
                     GKQIFALQGSYLIRKLRAETNKRYLDCLGIASIKDCSVVTSTK"
     gene            complement(1932..2063)
                     /locus_tag="KPH93_003506"
                     /pseudo
     CDS             complement(1932..2063)
                     /locus_tag="KPH93_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     regulatory      2241..2334
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2417..2902
                     /locus_tag="KPH93_003507"
     CDS             2417..2902
                     /locus_tag="KPH93_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1690276.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT         1330 a          332 c          490 g         1047 t
ORIGIN      
        1 attatttttt taaataaaat taagaggtga ttttatgaat ttagattttc actactatgg
       61 tacatatctt gcagctaaaa tagctggtta taatgattct gatgcaaaga caattgccta
      121 tgcagcacaa tatgtagacg aatcgagtaa aaatatgatt cttgatgatg taaactttgc
      181 atttcctaca atacagacta atttagaatt tgaaaaatat tatgcagatt ttacctcatt
      241 gggttataaa tggaatcttg atagtttgaa tgaaataaag aaagtatgga taccatttca
      301 ttttttacct ggaaatttaa ataatcaagt taagtataat ggagttaaag agagtaaagg
      361 tttgacaaca aattggaaat ttaaagatgg agatgaccaa aaatttagat tgatgtgctt
      421 acctaatagt gaaacggttt ctgctattat aaatgatctt atttattttc atagtactga
      481 agaatataaa ttacagttta ttggaataag aatgcatgta cttgcagata catgggcaca
      541 catgtatttt attggaaagc cagaatggta tattaatgat attaaagaat ttatagcaga
      601 agaaagtaaa tacatagaag aaactaaatg gaccaaagca gaagaatatc gtaatagcag
      661 ttggcattct attaacttta ctggtactcc tgatgttggc ggatatgaga gtatttcata
      721 tcttgggcat ggaagaattg gtcatctacc agattatgga tatcttaatt atagatatgt
      781 tccaaattgg agttgtactg ataacaaagt gaagatacaa aaaaataatc aagataattt
      841 ctttaaagca ttttgtcaaa tggtctatgc attaaaatgt attaagaacg gaaatgactt
      901 tagtataaat caatatgata atttaactgc tcaacaaaaa acagaggttt caaaagtcat
      961 agcaacaaga aaagatgacc aatcagaagc atggaaaaaa gcaatacaaa ttttagggta
     1021 taactcctta gaaaattttg ataaaaacaa gtggaaaaat gaatttatta aatctactaa
     1081 taaagaaaaa acagactact attattttaa tctagtagct aagaaacatg taaattatgt
     1141 aattgacttt ttagaggaaa agagactacc attagaagaa atgtgtagta aatattgtga
     1201 atttaaaact tttgatgata cacaatttgc taaaaataga aatagagtaa caaagattat
     1261 acttagagga gcttatatag ttgatgctat acaacttgta tatgacggaa aatataaaac
     1321 tccaatgcat ggtgaaacat taggaggctc aatggtatct cttgatttag aagctgatga
     1381 ttacatagtt aagataagcg ggtctatggg cttatatgaa ggtggagagc cctaccctaa
     1441 atcaccaact agaacaatag gaaaaataac ttttttaact aaaaaaggaa aaacaataac
     1501 aacagggtgt gaaactatgt ttaaatcata tggaaatttt actttagaag cacctgccgg
     1561 aaaacaaatt tttgcacttc aaggttcata tttaatacgt aaattaagag cagagactaa
     1621 caaacgatat ttggattgtt taggaattgc aagcataaaa gattgttcag tagttacaag
     1681 tacgaaataa atacatattg gatagctaat gttataattt atttaagaaa atggcacagt
     1741 attttatgtt tatatttaca ttaagagcac tctttttata tgagtgctta tttttttgaa
     1801 attcattagt atataaacta tcaagaacgt tactcaatat accttattta cttcaccaat
     1861 gactatctta tatatactaa caataaaaaa agaccctaaa aagagtcttt cccgaaaaat
     1921 ctatatatat attataatat agttatattt tctgcttgag gacctctagc acctttaact
     1981 atattaaagc ttacttgttg accttcttct aatgatttaa atcctgaagt ttatatagct
     2041 gagaaatgag caaatacatc atcataaaat ataagtaaca ctatagataa attactgtct
     2101 tttatattga ctataaagaa cagtaattga ataaatataa agatattttt tgattattat
     2161 taattaaaaa tttaatttat atttaaataa tataaaattt gaggtaatat tattttttaa
     2221 ttgttcaaat tatgttatta ttcagatata ggcaatatta gataaatcta atgacgcaaa
     2281 actataggga ctaaaatctt tatgtaataa aggtcatgtc agccagttgc caaaaagata
     2341 ataattcttt ttgttaaaag tatttttaga gttatatatt ttaataactt aatatttggc
     2401 aagaggaggg cttaagatga tatctgaatt aatgttaaaa aattttggca aatctagcat
     2461 caattttaag actaaaatta aattgaattt aagagaaaaa ccagacataa atagtttgaa
     2521 attaaaaagt ataccagaag gtaaaattgt taagttaaaa tgtgtagatg gaatatgggc
     2581 agaagttgag agtaattatg acaaaggctg gttactttat aaatatttag aaagattaag
     2641 taatgctaaa aacaatagta atgatacaat aaaaaataga aaaaagaatt acataggaaa
     2701 tataagaaca aatggattaa gtttagaact tagaaatgat agaacattag aatctaaagt
     2761 tataactaca atccctgatg gttttaaagt tgaagtatgt tatagtgttg gaaaatgggc
     2821 aagagttaat atcaataaaa atgaaaaacg atattctgga tatgtatata atcaatacat
     2881 agaagaacat ttaagtttat aatacgatac tagtgagctt taaatatatt aataaatcac
     2941 aaaaaagaat cccgttaatg ggattctttt ttgtagaaac aatataacag aactaaagag
     3001 aataaattaa ctaatttatt tttactataa agtaattata aatggtaaat tcaattgaaa
     3061 gtagatttga cttatacttt atacaccaag tatcataaat taatactact ttgaaaaatt
     3121 ttaaaaaaag taaaagttat gataataaaa ataatattaa agataataag atatataaga
     3181 aacttgaaaa aatttgtaa
//
LOCUS       DAEMAE010000305         3191 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEMAE010000305 DAEMAE010000000
VERSION     DAEMAE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.310"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            233..1675
                     /locus_tag="KPH93_003508"
     CDS             233..1675
                     /locus_tag="KPH93_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1690277.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVSGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1717..2070
                     /locus_tag="KPH93_003509"
     CDS             1717..2070
                     /locus_tag="KPH93_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690278.1"
                     /translation="MKKSSIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2261..3082
                     /locus_tag="KPH93_003510"
     CDS             2261..3082
                     /locus_tag="KPH93_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG1690279.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEKIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1239 a          327 c          553 g         1072 t
ORIGIN      
        1 tttttattac aaattgttac taaatataga tacattagaa caaaaatcta gtaaattgta
       61 actaaaatgt aaatttttac tttaatctat acaatattat ggaaactttt tgtaatttat
      121 gatataataa caaatatata attattaaat tataataaat gactatagcg tagatgtact
      181 actatgttat agtcattttt gaattaaact tgatatatca agggggaaga atatgagaac
      241 aattagaaga gttttagcat taggtttaac attagctata tttctaataa atgtacctaa
      301 tgtagatgca ttaacatcag atacaattaa aggtaacaat atatatgaga cggctggatt
      361 aatagcagat aaaaagagtt atgatacagc aattatggtg aatatggata attctatagc
      421 agatggcctt tcagcaagtg gtcttgcagg tgctgttgat gctccaattt tgcttgcaca
      481 aaaaaataag ataccaaatg aaacaaaaca aagattaaaa aatgttaaaa agatatacat
      541 aataggtaaa gaattatcaa taagtaagtc agtagaaact gaactgaaaa acacaggggc
      601 acaggtaact agattgggtg gagatgacag aataaagact agttatagtg ttgctaaaga
      661 ggtaagtggt atcaaaaaag ttgatgaagt gatattaact aatgcatata aaggagaagc
      721 agatacaata agtgctgcac ctgtatcagt aagagatata gctcctatag tacttacaga
      781 tggaaagagt gtgccttttt caacaagtaa tgtaaaaaca tatgcagtag gtggaagtat
      841 atccatgagc actagtttag ttaataaaac taatgctaaa agacttggtg gttcagatag
      901 atatgataca aataagaaag ttataaaaga gttttatcca gatgcatcag aattttattt
      961 aagtgatgga tatgatttag taaatgcact tacaggttct acaattgcta aggaaaatcc
     1021 aattgtatta gtgtcagaaa gtagtgataa gtctatatta gcaggagcag ataagattac
     1081 tagattaggt tcaataagtg acagtgtgta taataagtgt gtttctgctg cacaaaataa
     1141 tggcgattcg tctacaaaag gtgagtcacc tatgaaaaat gagacaagca tattaggtca
     1201 gccaacagct agtttagaag cgtgcttaaa atgggccaaa tctaaaaaag ctaatgattt
     1261 atttatagag ttaataccaa tattatatga tactgctgtt caggaaggtg ttaatcctgt
     1321 tttggcagta gctcaatctg caaaagaaac tggtttttgt aattttggtg gagtattaga
     1381 tgcatcattt aagaatcctt gtggacttaa aacttctgta ggtggttctg atactgataa
     1441 gaatgcccat tcaagatttg atacttggga agaaggaata ttagctcaaa ttcaacattt
     1501 atgcttatat gcaggacaag atggttaccc actttcaaat ccagtagacc ctagacatga
     1561 aaaatcttta tttggtaagg ctaaaacagt tgaaagtcta tctaataatt gggctggagg
     1621 tcaatatgga caagatttag taagaatgat gggagaaatt gaggcgacaa agtaattgaa
     1681 gattatcaaa tatccaataa ataaggattg attattatga aaaagagtag tatttttagt
     1741 atcatatttc ctacagtcat aatggtgcta atgtttttaa cttttgctac agatatattt
     1801 aatattccag atattcaatc taaaagtatt tttgttgtgg gaatggtttt gatttttcca
     1861 atttcttttt tgatacaagg aataatttgt gtattaaata agactaattg gattttatct
     1921 cttattgtgt ctttagtaac ttatatagta ttaatgtata tatttttaaa tgactctgca
     1981 tatatatatg ttttacttta tacagctttt tatatgattg gacatataac agctaaactc
     2041 tattacaaaa ttaagacttt taaaaattaa attgaagtat ataaaaataa tattaagaga
     2101 agtatcttta atgattttct aaaatcaaaa ataggtgctt ctcttttttg taaaaataaa
     2161 agtatgtaaa taaaatttaa ccattataat tttcttttaa caaatgtata caataaactt
     2221 gttgtataat taagatagat aaaataaagg gagggataat atgaatttaa tagataatca
     2281 tattcatact aatttttcaa gtgatggaaa ggattccatg gaagatacaa taaaaaaagc
     2341 tataagtatt ggtgttagat atttaacttt tacagaccat ttagaacatg atgaagatag
     2401 aggattttgc ataaattata atgactacgt tccagtattt aataagttta aagaaaaata
     2461 taaaaaggac atagagttat tactaggtgt tgaagtgggt tatagaaaac atctaaaaaa
     2521 tgaaatagaa aaaataataa attctaatcc ttttgatttt gtattgtgtt caactcatac
     2581 aattgacaat gtacctgttc catcaaaggc gtattttaaa ggacttagca aagaagatgc
     2641 atattataaa tattttaata gcatacttga aactaatcgt gaatttggag attacaatat
     2701 atatggtcat ttggattata tatctaggta tggaatatat tcagataata gagttatata
     2761 caatgacttt aaagatgtta ttgatgaggt cttaaagtca ataatcaata atgggagtgg
     2821 tatagagcta aatacttctg gttatagata tggactaaat gctatacatc caaatgaaga
     2881 tattctaaaa agatataaag aacttggagg atttatagtt actgtaggtt ctgattctca
     2941 tagggtagaa gatatatgta aggattttga tgtggcctat gatatgctta agtatcttga
     3001 ctttaaatat gtatctttat ttaaagaaag agaaacttat tttgtaaata tagaaaaagt
     3061 taaatctaac aatatagctt agagtgtgtt ttgaaataaa ttaatggata gttaaaaagt
     3121 taatagttat aatattgtat cctctagaaa aatactagag gtttttattt tgctaaaata
     3181 actaaataga a
//
LOCUS       DAEMAE010000306         3184 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEMAE010000306 DAEMAE010000000
VERSION     DAEMAE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.311"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            217..1509
                     /gene="eno"
                     /locus_tag="KPH93_003511"
     CDS             217..1509
                     /gene="eno"
                     /locus_tag="KPH93_003511"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG1690280.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(1609..2040)
                     /locus_tag="KPH93_003512"
     CDS             complement(1609..2040)
                     /locus_tag="KPH93_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1690281.1"
                     /translation="MSMNAIKELSNLWHIVGMQMKTEMCSGKYPTIYGISLVELSILE
                     IVEQHPNSMMKEISSTLELPKSTLTSAVKRLELKKLIKRNPCIKDKRAYNLDLTELGL
                     KAQQEHREIEATVFKNILHQLDKDEIETFIHIFSKAVNKIR"
     gene            2120..2629
                     /locus_tag="KPH93_003513"
     CDS             2120..2629
                     /locus_tag="KPH93_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1690282.1"
                     /translation="MNLEIIRATGENATEMGFIHSYSWKKAYKGIIPDNIIDEFTSEK
                     RAEIFHNVIPKAEEEYYLFKVDGIPAGFASLNKSHEENAPKYIGEIYSIYFHPDFWGT
                     PVTKKGLQFCIDRLRNLGYSYITIWVLKDNTRAIKFYKKNGFTCDDFEKEIYIGKKLL
                     EIRYSKKIQ"
     gene            2743..2967
                     /gene="secG"
                     /locus_tag="KPH93_003514"
     CDS             2743..2967
                     /gene="secG"
                     /locus_tag="KPH93_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG1690283.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT         1191 a          370 c          573 g         1050 t
ORIGIN      
        1 gattttagac aagttttttt atattatata aattaaaata ttataaaaaa taaaataaaa
       61 atatgaactt ttacaaattt acccatcaaa taaatttgta cctaattttc aaaatcaact
      121 ttatgtacta taatatagaa agtagattaa aatagtagac ggatgtttac agtaaatgaa
      181 aatttaacta atagaaaatc gaagggagat atacaaatgt cagttattga attagtatat
      241 gctagagaag tattagactc aagaggaaat ccaactgtag aagttgaagt agtattagaa
      301 gatggagcaa tgggaagagc aatagttcca tcaggagcat ctacaggagc ttttgaagct
      361 gttgaattaa gagatggaga taagggaaga tacttaggaa aaggtgtaga aacagctgtt
      421 gctaacgtaa atgaaataat agcacctgaa atagaaggaa tggatgcatt tgaccaacca
      481 gctatagacg caatcatgat agaattggat ggaactccaa acaaaggtaa attaggagct
      541 aatgctatat taggtgtttc aatggcagta gctagagcag ctgctgatga aataggtctt
      601 ccattattcc aatatttagg tggagtaaat gctaaacaat tacctgttcc aatgatgaac
      661 atattaaacg gtggagagca tgctgataac aacgttgatg ttcaagaatt tatgatatta
      721 ccagtaggag cttgttgttt caaagaaggt ttaagaatgg gagcagaagt attccattca
      781 ttaaagaaag ttttaggaga aaaaggatta gcttgtggtg taggtgacga aggtggattt
      841 gctccaaact taggttcaaa cagagaagct ttagaattaa tagttgaagc tataacaaaa
      901 gctggatata agccaggtga agacgttatg ttaggtcttg acgttgctgc tacagaaatg
      961 tacaacaaag aaactaaaaa atacgtttta gcaggagaag gaaaagagtt aactgctgct
     1021 gaaatggttg ctttatatga agattggtca aacaacttcc caataataac tatagaagat
     1081 ggtttagacg aagaagattg ggatggatgg aaattattaa ctgaaaaatt aggaaataaa
     1141 ttacaattag ttggtgacga cttatttgtt actaacacag agagattaga aaaaggaatt
     1201 gaaaatggag tagctaactc tatattagtt aaagttaacc aaataggtac aataactgaa
     1261 actttagatg ctatagaaat ggctaagaga gcaggatata ctgctgttat atctcataga
     1321 tcaggagaga ctgaagattc tactatagca gatttagctg ttgcagtaaa tgctggtcaa
     1381 ataaaaactg gtgctccatc aagaactgat agagttgcta agtacaacca attattaaga
     1441 atagaagaaa tggttggaga gcaagctaga tactgtggat taaaatcttt ctataactta
     1501 aaaaaatagt tattaagatt aaagagatag tataaaaggt ttaagtttgt cttatataag
     1561 gataagtgtt agttgatttt tcaattcact tatccttttt atattttatt acctaatttt
     1621 attcacagct ttagaaaaaa tatgaataaa tgtttctatc tcatctttat ctaattgatg
     1681 taggatattt ttaaatacag ttgcttctat ttctctatgt tcttgctgag cttttaaacc
     1741 taattcagtt aaatctaaat tatacgccct tttatcttta atgcaaggat ttctctttat
     1801 aagttttttt aattcaagac gttttacagc acttgttaat gtgctttttg gtaattctaa
     1861 agttgaacta atttctttca tcatactatt tgggtgttgt tcaactattt ctaaaatact
     1921 taattctact aaagaaatac cataaatagt aggatactta ccactacaca tttctgtttt
     1981 catttgcata ccaacgatgt gccataggtt gcttagttct tttatagcat tcattgacat
     2041 ttacctccaa ttatgatacg attatcgtac taatataata cgatagtcgt attaatttgt
     2101 caataggatg gtggttttta tgaatttaga gattatacga gctacaggtg aaaatgctac
     2161 agaaatgggt tttatccatt cttattcatg gaaaaaagcg tataaaggaa ttattccaga
     2221 taatataata gatgagttca cttctgaaaa aagagcagaa atttttcaca atgttattcc
     2281 aaaagctgaa gaggaatact atctttttaa agtggatggt atacctgctg gttttgcttc
     2341 attaaataaa agtcatgaag aaaatgctcc aaaatatatt ggagaaatat actctatata
     2401 tttccatcca gatttttggg gaactcctgt tacaaaaaaa ggattacaat tttgtattga
     2461 tagactaaga aatttaggat attcgtatat aacaatatgg gtcttaaaag ataatactag
     2521 agctataaaa ttttacaaaa aaaatggttt tacttgtgat gattttgaga aagagatata
     2581 tattgggaaa aaattattag aaatacgtta ctcaaaaaag atacagtaat tatatatttt
     2641 tttatatgtg atttatagca taatagtctt gaaatggcta tattattgtg atacactaaa
     2701 gagtaatgaa atttaagtaa taaacagata ggaggggcaa gaatgagcaa cattttaatg
     2761 ggtgtgcaag tagtactagg gattttacta gtaattagca taatgcctca agattcaaag
     2821 aatgctttac cttcagagtt tggtggagaa ggaagtcaag cttactttaa accaaaagga
     2881 aaacaagcat ttcttgctag agttacaaag ataacagcag tattattttt tataaatgca
     2941 ttggcattac ttatagttaa gaagtagtga tattgttgaa taactggcca ttatttaatt
     3001 agtgcatttg ctagacattt aaaaattaaa tttagttttt aataaaagta catcaacata
     3061 aaaagtacgt aaaataaaag ctggactaat gattttatat gattagtcca gtttttattt
     3121 tatagattga ctatctgtta gcatctattg tttgtatcta tatatttttt taaactaaaa
     3181 aata
//
LOCUS       DAEMAE010000307         3180 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEMAE010000307 DAEMAE010000000
VERSION     DAEMAE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.312"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            547..924
                     /locus_tag="KPH93_003515"
     CDS             547..924
                     /locus_tag="KPH93_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690284.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            917..1939
                     /locus_tag="KPH93_003516"
     CDS             917..1939
                     /locus_tag="KPH93_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG1690285.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIVVFRMLLFLFNYTIVYEVKESKENTVGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVIYNCSFNDKKEYALALIKSIKLSKECNRGILLTDFNRTNLE
                     KRLESIISKKFKNGVFVAIILCLLSSITYFKFDIFISKPVIYSLPKPSVLNDEYIYNT
                     DIRVER"
     gene            2001..2594
                     /locus_tag="KPH93_003517"
     CDS             2001..2594
                     /locus_tag="KPH93_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG1690286.1"
                     /translation="MLKKIKKVLSIACCLFILGGSISFADLENNNEINDKSVENPIYE
                     FNIQGYTFTYENAPQRIKDEYNKTCRELNITPSPSTEIFIPIDEISKYTDTVPLFEID
                     EFNVKYFGTRFEVTGAKTYTVGVSTYVGYGHTTSGNPVHLAQLLLKKSGYSIAIDSLF
                     GSSTYSKVRSFQSKYGLSVDGVVGLGTWNKLAQVTNA"
BASE COUNT         1275 a          290 c          442 g         1173 t
ORIGIN      
        1 ttataatgct aaaattaaaa aaatataata ctaaaatata attatgtttt aattaaaata
       61 aagaacttta ttagtagata ttaaaagcac atttttttat aaaagtgctt ttttataatt
      121 tattaaaggt ataattataa aaatcttatt caataaaatt tataattaat ttagatatat
      181 ttgtaaataa tcagtataaa atagatattt taatagcata aaggtataat taggaggtta
      241 ttaaaattta ttagatatta ctctagttta tttaaaaaaa aagtattata atattctttg
      301 tgtgaaaata taggaatttt tattaaatag ttttaatgtg taaataaaca ttctacacta
      361 ttgtaattaa atatgtaaaa agtaaaatat ttaaatggat atattttaaa atctaacttt
      421 taatattgta aaaaaattag aaatttaaca aaaagtacaa aaattcacat ttttttcata
      481 aagtgtttga ctaaaaaatt cacatgttgt agaattaagg tagcaggagt tttgagggag
      541 gtgtaaatga tgataagaaa aataccacaa gcagaactaa aagtaatgaa attcatttgg
      601 aaagtagatg ttacagtgac atcaaaagat gttattgaag ctatggaaca aaaatatggc
      661 tggaaacaaa ccaccacact gacactttta tctaggctgg taaaaagagg attcttagat
      721 gctcaaaaaa tagacagata tacacattac acaataagag taaaacacaa agattattta
      781 agttttgaga caaaggattt cctaagtaat atacatgatg actctttaag aagcttaatt
      841 tcagcattac atgatgatga aaatataggt aaagataagc tagattttct agaaagttat
      901 tttgataatt taaaggatga ataattatat gctatatgtt tttgaaaatc ttataaagac
      961 aactattgta tgtagtcttg gtatttgtct attattattt ttaaaaagat atttatttaa
     1021 gaagtttagt aagagattca attattatat ttggttgata gtagtcttta gaatgttact
     1081 ttttctattt aattatacga tagtgtatga agtaaaagaa tccaaagaaa atacagtagg
     1141 aaataatatc acacaaattg atatttctac agataataat cttatgctgt atgtagctta
     1201 tttatggtta tttgtaacta tagtcattgc agtttataca tttataaagt atacaagatt
     1261 taagaatctc gttgttgatg tatcttatga tattgaagat aatgatataa actgtatcta
     1321 caaaaatttg ttaaaagaat taaatataaa aaagaaaatt gaattaagag gttctgatga
     1381 attgataagt ccagcaggca tggggttatt taaatcttac atattcttac cagactatcc
     1441 ttatagtaaa gatgaactaa cttggatttt gaaacatgaa ttaatgcatt ttaaaaataa
     1501 agatatttta ataaagtttt tagtgttatc tgttagaata atatattggt ttaatccact
     1561 tgtttatgta atgagcaata aagtaaatct agattgtgaa ttatgttgtg atgaaagtgt
     1621 tatttataat tgttcattta atgacaaaaa agaatatgca ttggcactta ttaaatcaat
     1681 taaactaagt aaagaatgca atagaggtat tctattaaca gattttaata gaaccaattt
     1741 agaaaagaga cttgaaagta ttattagtaa aaagtttaaa aatggtgttt ttgtagctat
     1801 aattttgtgt ttactatctt caataacata ttttaaattt gatatattta tttcaaaacc
     1861 agtgatttat tcgttaccga agccaagtgt gttaaatgat gaatatattt acaacactga
     1921 tatccgagtt gaaagatgat gttaaattgt attaaaaaat gtaataattt aaataaaaga
     1981 aaatatttta ggaggatttt atgttaaaaa aaattaaaaa agtactatca atagcgtgct
     2041 gtttattcat attaggaggg agtatatctt ttgcagattt agaaaataat aatgaaataa
     2101 atgataaatc tgttgagaat ccaatttatg aatttaatat tcaaggttat acatttacat
     2161 atgaaaatgc accacaaaga attaaagatg aatataacaa aacatgtaga gaattaaata
     2221 taacaccttc tccatctact gaaatattta ttccaataga tgaaatctct aagtatacag
     2281 atactgttcc tctttttgaa atagatgaat tcaatgttaa atattttgga acacgttttg
     2341 aggttactgg tgcaaaaaca tatacagttg gagttagtac atatgtggga tacggacata
     2401 caactagtgg aaatccagta catttagcac agcttttact aaaaaaatca ggatattcta
     2461 tagcaataga cagtttgttt ggtagttcta catatagtaa agttagaagt tttcaaagta
     2521 aatatggact aagtgttgat ggtgtggttg gattgggaac atggaataag ttggcacaag
     2581 ttactaatgc ttagataatt tattataaag ctaaaaagaa acatgggagt ctggaaatat
     2641 tcatgcatat tcttttaatg aaattttatt tttaaaatgc caattaaata tatataaata
     2701 agactatgtt tataaaaatt atagtcttat ttatatattt attcaaaagt tattactcct
     2761 ttaaggaaaa tccagctaga ctaaatacat aagttattaa tgtattttta actcctattc
     2821 tgcaagtaat tttaggtaat tctttgaact tttattttct ctaattaata ttactaatgt
     2881 attctttata tcttataagt tgatatcaat agctatacac tgtattattt attattatac
     2941 ttttacttct aaatttataa aatgtttaat tcataaatat aattggataa ataatatcaa
     3001 atgttctatt ctatcatcca tgaatgaaag gtatacctat atcagtaatt ttagcaatta
     3061 gtgaatgaga tgtatcaatt ctgcaaatac ttttaaggta acttgaaata tttaaggtta
     3121 ttttttcaaa ctctgcttct aacaaattaa tgaattgtac ttatgaataa atattttaac
//
LOCUS       DAEMAE010000308         3177 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEMAE010000308 DAEMAE010000000
VERSION     DAEMAE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.313"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    115..377
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            453..3119
                     /locus_tag="KPH93_003518"
     CDS             453..3119
                     /locus_tag="KPH93_003518"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG1690287.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGRDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLANLESKIK"
BASE COUNT         1282 a          346 c          621 g          928 t
ORIGIN      
        1 ttaaaatata acaattagta taaaatataa taaatataat taagaaaatt cagaaaaaat
       61 gaaataaaaa taattgacaa agagaataat atttgtatat aataatatgt aatattaaag
      121 actgagaaga agaggagtaa gtaaatacta ctaaaagaga ggaaaattca ctggctgtaa
      181 gattttctaa gtataagatt tactgaatgt agctttggag tttctaaact gaactatgag
      241 taagtttaga cggttagaat cgttaaattc tttgagagtc aaagttgata aaataacatt
      301 aactaataat ttaattatat gttaatgtat tttaagcttt ggaattaagg tggtaacgcg
      361 agcttttcgt cctttttaaa gaggatgaag agctcttttt tatttctaaa gatatttaaa
      421 aaatttattt ataaaataca ggaggtagga caatggaaaa tacgaattta tcaaaaactt
      481 ataacccaaa agattttgag gctaggttat ataaaaaatg gatggatgaa gggtatttta
      541 aatctaaacc aaatccagat aaaaaaccat ttacaataat gatgccacca ccaaatataa
      601 cagggcaatt gcatatggga cacgctcttg accatacact tcaagatata ttaatcagat
      661 ggaaacgtat ggatggatat gaagcttttt ggcttccagg aactgaccat gcttctatag
      721 caacagaagt taaagttgtt gaaagaataa aaaaacaaga aggtaagaca aagtatgaaa
      781 taggtagaga agaattttta aagagagcct gggaatggaa agatgaattt ggagggaaaa
      841 tatctaatca gcttaaacaa ctaggtgatt cttgtgattg ggataaagaa agatttacta
      901 tggatgaagg gtgtaatgaa gcagttatag aattttttgt aagcctatat gaaaaaggtc
      961 acatatatag aggaaacaga ataataaact ggtgtccaga ttgcaaaact actttatcag
     1021 atgcagaagt tgaacatgaa gaacacgacg gaaactttta tcatataaag tacccactaa
     1081 aagatagtga agacttttta gaaatagcta caactagacc tgagacaatg ataggagata
     1141 ctggtatagc agtaaatcca gaagatgata gatataaaca tttaatagga aaaacagcaa
     1201 tacttccatt agtaggaaga gaacttccaa tagttgcaga tagttatgtt gatttagagt
     1261 ttggaactgg tgcagttaag atgactccag cacatgaccc taatgatttt gaagtaggat
     1321 taagacacaa tcttgagcag ttaaatacta tgaatgaaga tggaactatg aatgaagttt
     1381 gtggaaaata tgaaggtatg gatagatttg agtgtagaaa agccattgta gcagacttaa
     1441 aagaacaagg ttacctaatt aagataaaag agcataatca taatgtagga acttgttata
     1501 gatgccatac agtagttgag cctagattat cagagcaatg gtttgttaag atggaagaac
     1561 ttgcaaaacc agctatagat attcttaaaa aaggagaatt agaatttgtt ccagataaat
     1621 ttgataaaac ttatttacaa tggctagaaa atataagaga ttggtgtata tcaagacaat
     1681 tatggtgggg tcatcaaata ccagcttatt attgtcaaga gtgtggagaa atagtagttg
     1741 ctagaaaaat gccagataag tgtcctaaat gtggaagcac tcattttaaa caagatgaag
     1801 atgccttaga tacttggttc agttctgcat tatggccttt ctctactcta ggatggccaa
     1861 ataagactga agctttagat tattattatc caacaagtgt acttgttaca ggatacgata
     1921 taatattctt ctgggtagtt agaatggcat ttgcaggtat gttctgtatg aatgaaaagc
     1981 catttgacca tgttttggtt catggattag taagagattc tcaaggtaga aaaatgagta
     2041 aatctttagg aaatggtatc gacccattgg agataatcga gcaatatgga gctgatgccc
     2101 taagatttac attaacaact ggaaattccc caggaaatga tatgagattc tatatggaaa
     2161 gagtagaatt tgctagaaac tttgcaaata agttgtggaa tgcatcaaga tttgtattta
     2221 tgaatattga tgaagatata attaaaaaca tgactagaga aagtgttaaa gaggacttaa
     2281 ctctagctga taaatggata atatcaagag caaataatat tgtaaaagaa gcaactaata
     2341 acatggataa gtttgattta ggaatagcac ttcaaaagat atatgatttt acttggtcag
     2401 aatactgtga ctggtatatt gaaatggtta aacctagatt atatggggaa gatgcaaatg
     2461 ctaaatctgc agcattatat acattaactt atgttcttga aaagatatta aaattattac
     2521 atccatacat gccatttata acagaggaaa tatatactca tcttcctact gttgaaggat
     2581 gtataatagt atctgaatgg ccaaaatata atgaagaaga taacatggct gaagaagaag
     2641 atatgatgaa cttattgatg gaaggtataa gaagtataag aaatgtgaga gcagaaatga
     2701 atgtaccacc ttctaagaaa gctaaattga taataatccc aagtgaagaa aaaatagaag
     2761 ctattgagct tggcagagac tattttataa ctttagcatc tgcttcaaat gtagagatag
     2821 caaaagataa atctaatgtt ccagaagatg cagttggagt agttattgat ggtgttgaaa
     2881 tatttatacc tcttaatgaa ttagttgatt ttgaaaaaga aattgagaga ttatctaaag
     2941 aaaagaaaaa gttagaaggc gaaataaaga gagttaatgg aaagcttgca aatcaaggtt
     3001 tcttagcaaa agctcctgaa agtttaattg aagaagaaaa agcaaaaaaa gaaaaatttg
     3061 aagagatgat taaatctgtt gaggaaagat tagcaaattt agaatctaaa ataaaataaa
     3121 tataagaatg agtaaaaaca tacaaaaagt atatattcta tactttttgt atgtttt
//
LOCUS       DAEMAE010000309         3100 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEMAE010000309 DAEMAE010000000
VERSION     DAEMAE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.314"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1..496)
                     /locus_tag="KPH93_003519"
                     /pseudo
     CDS             complement(1..496)
                     /locus_tag="KPH93_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(498..605)
                     /locus_tag="KPH93_003520"
     CDS             complement(498..605)
                     /locus_tag="KPH93_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1690288.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(1237..2394)
                     /locus_tag="KPH93_003521"
                     /pseudo
     CDS             complement(1237..2394)
                     /locus_tag="KPH93_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(2437..2832)
                     /locus_tag="KPH93_003522"
     CDS             complement(2437..2832)
                     /locus_tag="KPH93_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690289.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTSKYEQEVIKTETIKT
                     AEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
BASE COUNT          928 a          495 c          403 g         1274 t
ORIGIN      
        1 cataatgata cataatatct accacttggt tctttagata tagtagcatt aagtattctc
       61 ccttgaggta cttgtttatc acttatctta accataccaa gtttaggtag ttttatatgt
      121 tgactaaagt acataatgtt tccatttgta aagttagttt tatatgaaaa tctattagtt
      181 ttctttgatt taaatttagg aaatcctgcc ttttttttaa agaattttca tatgcattat
      241 ctaaatcttt taaggcgttt tgtagtgaaa atttgtcagg ttccttaagc caatttaatt
      301 ctttttttaa attggtcaaa tcagaactac actgtttata agtaaaagtt tctttatcgt
      361 ttttataaac atctattctt ttagcaagat atttattata tacaaatcta caacatccaa
      421 aagttttttt aattagctct tgttgttttt tatttggata cattctaaac ttataagctt
      481 tttccactac tatcacctca ctttacttgt tttttctgat tattaatata ttttcttatc
      541 tgctcctctg tattttctga tacagtagca acgaaataac taggattcca aagatgtctc
      601 cccataatac tggatgtcta tatttgacat actatatgca tatcctctac catgagtaac
      661 ttctactttt catcacctat cataagatta aaatattcaa ttacttaaaa caagtatttt
      721 gaaggttttt tatatttatt tttaaataac atatataaga accgtaagtc ttaactttta
      781 tcatttttga ggttgtcgtt cacataagtt cgctactacc tatgcagttc tcttatgaac
      841 ttcttacact ttcatgtaag cacagactat atcttatcct tcggcattat ccgctaaggt
      901 ctacccactt ccactaccaa tagcttgtag tgtacttccc tcaagaggaa tagtcgttga
      961 agtttctcct attcggagct tacctgctga ttgcccattt taaagtactt aggatttaac
     1021 cttataccat ctaaccaatt ttttctactt tcgtaacttt cacacttagg tttatttcat
     1081 ccttatgttt tagtttggtt agctttaggg gtttttagca attcaagtag tattggatag
     1141 gtgttatcct atctctacat ataagtttcc ctatatgctg actaatttat ctactaactt
     1201 atgactaaag tcacaagtat gcgtagctat tttttaatca aatacaatag tatctgcaca
     1261 tgcaacacct tcaaggaatt ttccaccttt tacagtaatg ttgtgattgt tacattctct
     1321 catggctgat agtactgaaa tagggataga gagttttaat gaacaagaga aatctaccat
     1381 taatatagag agtgatttta tagggtttct agtaattgca tatgttaata tacttactaa
     1441 aaagctgtat ggaactaatg tatctgctag atgttcaaat ttactctcta cagaagattt
     1501 tagtttttca gattcctcaa tcatagttac tattttctgg aatctagtag taccagcttt
     1561 ttcctttaca cacataacaa tattgccttg ctctattaca gttccagcat atatgtaaga
     1621 gctttctttt ttgttaactg caagggattc gcctgtaagt gatgcctgat tgaccattgc
     1681 ttctcctgat actataactc catccaatgg aatcatattt cccatagtta tactgactaa
     1741 atctccttcc tttatctctg aaattggaat tagtatttca gtgtcacctt ttcttaacca
     1801 tactttttca atgtttagtg acatactttg agccaagtcg tttatagatt tttttctagt
     1861 ccattcttct aataattcac cgataccaag aagaaacata actgaaccag ccgtattgat
     1921 atcttttcta aaaatagaaa tacctatagc agtggcatct aaaacagaaa tttctatttt
     1981 tttatttagt agggaagaga gacctttttt aaaatatttt atagaatgaa gtccaattaa
     2041 aaaaaatctc aaggactttg ggaaaaatat tttggatata cttttgacta gcagtttatt
     2101 tactaatttg tctctataat aattgtttaa ttccctactg gtatgttcag gaactagatt
     2161 ttctattttg ctattgttaa aactgaattt agtgatttct cttattatgt attctctttc
     2221 accagtgtaa taaatgactg catcaccagt tctatcgtaa acttttgctt tggtaatacc
     2281 atttatagaa gttaaataat ataagagagt atcggactgc ttgttgttta atttgccaag
     2341 ttgtaagtca aagcgtatac gacctcttat ttcgtgtttt atataaaaat tcaatttact
     2401 tcctcctgat attattaatc ataataatca ttaaatttat tctgttgtac aagtactatt
     2461 tttttcttca attacgttat ctttgatttc ttcacattta atgctattag aattagtatc
     2521 ttctgcagtt tttatagttt cagttttaat aacttcttgt tcgtatttag aagtatcttg
     2581 tacaacgttt accttagatg tagataagtc ttcttgttcg ttgatttctt ttgcttcagc
     2641 taaaatatct tctacatttt cttgaatttt tgtagttgtt tcaagaacag aatctttaac
     2701 tttcaatgct cctgctgttg cacgagtgca agttttttta acacagtcac ttgaaagtat
     2761 cttaacacct acagtaccaa aaagtactcc tcctaagaat atccctgttt ttttcatttt
     2821 taaaatattc attttacatt atcctttcat ttagttagat tttgtttgta cttatgtata
     2881 aggtaacaaa tgctaaaaat agagtatgtt ttacaaaatt agtttagaga gtttagtaat
     2941 tttatttgat ttatattagt aaatttatac gagttttaca tgtgtagttt atgtagttga
     3001 aataattctt atctagttta tatatgtaat ttatatatgt agcttatata gttgaggtaa
     3061 tactttatat agcttataga gttcatataa atttataacc
//
LOCUS       DAEMAE010000310         3098 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEMAE010000310 DAEMAE010000000
VERSION     DAEMAE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.315"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..165)
                     /locus_tag="KPH93_003523"
     CDS             complement(<1..165)
                     /locus_tag="KPH93_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690290.1"
                     /translation="MDFGNIYLVLIGIAVIIVTTIRYLIKGKTNYCKKKYLDKLELKY
                     GNIDREKVVKL"
     gene            complement(194..1180)
                     /locus_tag="KPH93_003524"
     CDS             complement(194..1180)
                     /locus_tag="KPH93_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1690291.1"
                     /translation="MKLQEKWIDLLNPKYIENKEKRKKFQIIIFFSITFGLTYLLGLL
                     LYFNKFIDPENFALFMMVLPLSSVAIAKFYTEGMTNDKYKFYSLIILFFLIYLFLLII
                     ELLNLINNQQFQSLNTILVIISSLSIILYSDSIKDLSPIKNIKVGCLLIFYFMLSKII
                     PESLRLIIQGNQPNYEGILNYIFSYMMLFFSIYPFFGEEYGWRGFLQDIFFDRLGKKL
                     GILALSMCWSLWHLPLIFTLYTPETPVLGLITRSIYIFGISIFLGYVYMKTKNIWFCA
                     IIHALNNTSFLISASSITYCSTINYSNIVEISILILIFYVPFLFTKEYKQNT"
     gene            complement(1595..1795)
                     /locus_tag="KPH93_003525"
     CDS             complement(1595..1795)
                     /locus_tag="KPH93_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690292.1"
                     /translation="MCFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     IIDEVNNVVWRNEKSTPAHENA"
     gene            complement(1814..2227)
                     /locus_tag="KPH93_003526"
     CDS             complement(1814..2227)
                     /locus_tag="KPH93_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690293.1"
                     /translation="MKVKNKRNFIVGIIVCMLCCASLVIYCILKEKRFLISSFILITI
                     AIFNFCNAFSRKGIVEELHNSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLIVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(2211..2444)
                     /locus_tag="KPH93_003527"
     CDS             complement(2211..2444)
                     /locus_tag="KPH93_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690294.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            2663..3037
                     /locus_tag="KPH93_003528"
     CDS             2663..3037
                     /locus_tag="KPH93_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690295.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRKLVAAMEEKYEWNESTT
                     FTVLKKLERREFLSTQKIGKLTHYNILVKEKNYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
BASE COUNT         1231 a          387 c          360 g         1120 t
ORIGIN      
        1 tagtttaaca actttttctc tatctatatt tccatatttt aattctaatt tatctaaata
       61 ttttttctta caataattag ttttaccttt aattaaatat cttattgtag ttactattat
      121 aactgctatt ccaataagta caagatagat attaccaaaa tccatagtag atacctcctt
      181 tataatataa tctttaggtg ttttgtttat attcttttgt aaatagaaat ggaacataaa
      241 aaattaaaat aagtattgat atttctacta tattagaata attaatagtt gaacaatagg
      301 taatactaga agctgatata agaaaagatg tattatttaa agcgtggatt atagcacaaa
      361 accatatatt tttagtcttc atgtatacat atcctaaaaa tattgaaatc ccaaatatgt
      421 atattgagcg agttattaag cctaatactg gtgtttcagg tgtatataat gtaaatatta
      481 gaggtaaatg ccacaaactc caacacatac ttagtgctag aataccgagt tttttaccta
      541 atctatcaaa aaatatatct tgcaaaaatc ccctccaacc atattcttca ccaaaaaaag
      601 ggtatataga gaagaataac atcatataag agaatatata attcaaaata ccttcatagt
      661 taggttgatt tccttgtatt ataagcctaa gtgattccgg aataattttg gaaagcatga
      721 aataaaaaat tagtaaacaa cccactttaa tattttttat aggagacaaa tcttttatgc
      781 tgtcagaata aagaataata gataaactac ttataataac caatatagta tttaatgatt
      841 gaaattgctg attatttatt aagtttaata attcaataat aagtaaaaat aagtaaatta
      901 gaaaaaataa tattattaat gaataaaatt tatatttatc attagtcatt ccttctgtat
      961 aaaattttgc gattgcaact gaggataagg gcaataccat catgaataat gcaaaatttt
     1021 ctgggtcaat aaatttattg aaatataata gaagacctaa taaataagtt aaaccaaaag
     1081 ttatagaaaa aaatattata atctgaaatt tctttctttt ttctttgttt tcaatatatt
     1141 ttggatttaa taaatcaatc catttttctt gcaatttcaa tataaaaacc tcctttaaaa
     1201 tatcactttc tttttttgta atcaccaact tatattgtat taaattaaat ttcttaatca
     1261 aagtatatca gtaatacaag tataaagagt atctaaaata ttacaaatat taacaagaca
     1321 catcataatc tgtaataata aaaagaatga aaattatcat gctaaaacat aagacatgtt
     1381 ataattatat tacatatttc ataaatatac atttaaagca ctctttttat atgagtgctt
     1441 atttttttga aattcattaa tatatatata aactatcaag aacattactc aacacacctt
     1501 aaaatcgatt taaataagtc tttttaataa cataaattat atgatataat aaaaaaaata
     1561 gaagtgtatt ggtagtacac ttctatagtt ataactaagc attctcatga gcgggagtgc
     1621 ttttttcatt tctccaaaca acattattta cttcatcaat aattatcaaa tcaatctcat
     1681 tatctttcaa aatctctgtt gcagtacaaa tgagattatc taagtcaaaa cttcgagtta
     1741 tctctagttg tttgttattt acttctcttt ttagtatata agttttaaaa cacatttata
     1801 tcacctcttg tatttactct tttttctcta aaaatatatt gataagtaaa tatgcaacaa
     1861 ataaaaaaat ctcaatgaca catagtgtta tagctataac tataagtgat tgatttttcc
     1921 atgcactata agctatgatg aaaagaaatg taaaagtaaa taatgtataa ttcataattt
     1981 taactaagat atgacttgtt ttcattgtta agtacaagtc tctttcatct gtactattgt
     2041 gtaattcttc aacaatgcct tttcttgaaa aagcattaca aaaattaaag attgctattg
     2101 taattaaaat aaatgatgaa attaagaatc ttttctcttt taaaatgcaa taaataacta
     2161 aactagcaca acataacata caaactataa tacccactat aaaatttcgt ttattcttta
     2221 ctttcatgtt ttttatcctc catttcttta ttttctttta aacaacatag ttcttcaata
     2281 gttgtaccaa atacttgtgc catacgatag gcaagcataa gagaagggct atattgctct
     2341 ttttcaatag aaataattgt tctgtttgat acatggacta aatcagcgag ttgttgctgt
     2401 gttagaccca tatttgttct aaattctcgt acttttgttt tcattccata acctctttct
     2461 atatgaagct aacttcatgt gaagttaact tcataatatc ctgaaatgag taaaatgtca
     2521 atctcattag ataagtatta taaaacctaa aagaataata tggtaaaaat tagatggata
     2581 tatttatatt aattctcttg tttggaaaac tttattaaga tagaatttaa catatatata
     2641 aatcggcaaa ggggtgcgaa ttatgaaaat tagtaaatta ccagaagcgg aattaaaagt
     2701 tatgagatat atttgggaag ctaacaaggt attaacttct aggaaattag ttgccgcaat
     2761 ggaagaaaaa tatgagtgga atgaatcaac tacatttaca gttttaaaaa aattagagag
     2821 aagggaattt ttaagtacgc agaaaatagg taaacttaca cattataata tactagtaaa
     2881 agaaaaaaac tatctaagat ttgaaacaaa ggaatttttg aagaacatac ataaaaattc
     2941 aatttcaagt ttaatgtcag cgttacatgg tgaagacgac gaagtggatg aagataaatt
     3001 aagtgaactc gaagaatatt ttaagaattt gaaataagaa aatgacaaat tgatacataa
     3061 taaataaaat gaaggtatga aattataact ttttctac
//
LOCUS       DAEMAE010000311         3068 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEMAE010000311 DAEMAE010000000
VERSION     DAEMAE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.316"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..1199
                     /gene="recA"
                     /locus_tag="KPH93_003529"
     CDS             153..1199
                     /gene="recA"
                     /locus_tag="KPH93_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG1690296.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            1375..2916
                     /gene="rny"
                     /locus_tag="KPH93_003530"
     CDS             1375..2916
                     /gene="rny"
                     /locus_tag="KPH93_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG1690297.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         1291 a          322 c          636 g          819 t
ORIGIN      
        1 taattattaa taatggttta taaataaaat gtaatgaata aaaatataat cattagtagc
       61 gtttttaaat tgactaggtt tgatttatgt tatataatta atatataaca agagaacaaa
      121 tgtttgtaga taaatgaagg gatggtatag agatgagtgt agatcaagaa aaattaaaag
      181 cgttgaatga agctttaggt aaaattgaaa aagattttgg taaaggttca gtaatgaaat
      241 tgggagaagc aacatctatg tctatagatg ttatatcaac aggagcgatt ggtttagaca
      301 tagctattgg tataggaggt ctacctagag ggagaatagt tgaagtatat ggtccagaat
      361 cttctggtaa gactactgtt gcgcttagtt gtgtagcatc agctcaaaaa gatggaggaa
      421 tagctgcatt tatagatgca gaacatgcac ttgacccagt atatgcaaaa gctttgggtg
      481 tggatgttga caacctaata atatctcaac cagatacagg tgaacaggct ttagagatag
      541 cagaggcatt gataagaagt ggagcgatag atataatagt aatagactca gtagcagcat
      601 tagttccaaa ggctgaaata gatggagata tgggtgattc tcatgtaggt ttacaagcta
      661 gacttatgtc acaagcactt agaaagttaa ctggttcaat taaaaaatca aattgtgttg
      721 ctatatttat aaaccagtta agagagaaag taggaataat gtttggaaac ccagaaacta
      781 ctactggagg acgtgcacta aaattctatt catcagttag attggatgtt agaaaaatag
      841 acacaataaa acaaggtgat aaagttatag gtagtagaac tagagttaaa gttgttaaaa
      901 acaaagtagc accaccattt aagcaagctg aatttgatat aatgtatgga gaagggattt
      961 caaaaattgg agacctttta gatatagctg ctgatgtaga tatagttaaa aaatcaggtt
     1021 catggtatag ttacaatgat actaaacttg gacaaggaag agaaaatgtt aaaaaattct
     1081 tggaggataa tttagattta actactgaaa tagatgagaa agttagagca ttttacaatt
     1141 taaatgaaga acatgaagaa tcaggtactt cagtatcaaa agaaattgta gaagaataag
     1201 taaaaataca aaaaaataac tagtaattaa agacatgcca cgctccctat tagcttgaca
     1261 caatgttgaa aaaattataa aattaagtga ggataaaata tctaaattta attttaatta
     1321 ttaggaatat tttttaatca tagtgatgac attttgggat tattaacgaa ggaggtggat
     1381 gtcatagata gtgtagtaat gattgtaata ggtgcagctg ttggtattat agctggatat
     1441 tttgttagaa aaaatatatc tgaagcaaaa attggacaag ctgaaaactt ggccaaagaa
     1501 ataatagata aagcacacca cgactcagaa acagtacaaa aagaaaagtt actagaagct
     1561 aaagaagaga tccataagtg gagaacagaa gctgaaagag aaaacagaga aagaagaaca
     1621 gaagtacaaa agtatgaaaa aagagtagta caaaaagaag aagtcttaga tagaaaatta
     1681 caaaatttag aatctaaaga agtaaaccta agtgaaaaat taaaggtagt ggaaaagaaa
     1741 gaagaagaag ttgaagtaat aaaaactcaa cagttagaaa aattagaaag tatatcagga
     1801 attacttctg ataaggccaa agaaatcatc ttaaccaatg cggagagaga tgtaagacgt
     1861 gaaatgtcta taatgataaa agagatagaa agtcaagcta aagaagaagc tgataagaaa
     1921 tcaagagaaa ttataggata tgcaattcaa aaatgtgcag cagatcacgt agccgaaact
     1981 actgtaacag tagtaagctt gcctaatgat gaaatgaaag gtagaataat aggtagggaa
     2041 ggtagaaata taaggacttt agaaacctta acaggtatag accttataat agatgacact
     2101 ccagaagccg taattttatc aggatttgat ccaataagaa gagaaattgc aagaattgct
     2161 ctagaaaaat taatagcaga tggaagaata catccagcta gaattgaaga aatggttgaa
     2221 aaggcgagaa aagaagttga caatattata aaagaatatg gtgaacaagc tgcatttgaa
     2281 actagtgtac atgggattca tccggaatta ataagattat taggtagact taactataga
     2341 actagttatg gacaaaatgt acttaaacat tctatagaag ttgctcatat agctggtata
     2401 atggctgctg aaataggagc agatattaga ttagctaaga gggcaggttt attacatgat
     2461 ataggtaagg ctgtagacca tgaaatggaa ggtacacatg ttgaaatagg tatggattta
     2521 cttagacgtt ataaagagtc taaagaagtt atacatgcca tgagtactca tcatggagat
     2581 tacgaacctc aaacagtaga ggctgtatta gttacagcag cagatgcaat atcagctgct
     2641 agacctggtg cgagaagaga aactttagaa gcatatataa agagacttga aaaattagaa
     2701 gaaatagcta attcatatga aggtgtagaa aaatcatttg ctatacaagc tggtagagaa
     2761 ataagaataa tggtaaaacc agaagctata aatgatgaag aaatacatct attggctaga
     2821 gatatgacta agaaaataga ggatgaactt gaatatcctg gacaaataaa agtaagcata
     2881 ataagagaaa caagagctat agaatatgcg aagtaaaaga aataatatct aaataataag
     2941 aaaatatttt tagaaattta aatttgccac aagatatgat aatatgtctt gtggctttta
     3001 aattgtttaa gtttaatttt atataatgaa aaaataatct aggtgctaaa agtgctactt
     3061 ataaaaga
//
LOCUS       DAEMAE010000312         3067 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEMAE010000312 DAEMAE010000000
VERSION     DAEMAE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.317"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..426)
                     /locus_tag="KPH93_003531"
     CDS             complement(<1..426)
                     /locus_tag="KPH93_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690298.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCIAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLF"
     gene            complement(1235..2272)
                     /locus_tag="KPH93_003532"
     CDS             complement(1235..2272)
                     /locus_tag="KPH93_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1690299.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2369..>3067)
                     /locus_tag="KPH93_003533"
     CDS             complement(2369..>3067)
                     /locus_tag="KPH93_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1690300.1"
                     /translation="TIFENLSIDIEDGEFVSIIGSNGTGKSTLLNIISGLIKESSGQI
                     TLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVRENLSLALNKGKLLNLKKCLRH
                     KTNYLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIMSSLASPKVLLLDEHTAALD
                     PKTSNEVIGLTDKIVREKNITTLMVTHNLKHALQYGDRLIMLHKGEVVLDVKDKEKED
                     LTVEEILEKFEYAV"
BASE COUNT         1051 a          473 c          397 g         1146 t
ORIGIN      
        1 gaataggtct aaaaggactt tacagatgaa tacaaaagct aaggctaaaa ctataggaga
       61 tatttcccca ttgaataagt gtttaaagct aaatagagga atatttgatt ttcccattat
      121 tcttaaattg atagagtata gcattcccat aactaaaata ccagaaagta aattagatat
      181 cttaaactta atgtgaagaa tccctgtaac aagaccagca atacaaccac ataagatagc
      241 cattaaagtt gctactacag gagaaatacc attagtaaga gaaaatgcta cgatagaagc
      301 acccattgtg tagctaccat cagcagacat atctgggaaa tctaagattt tgtaagttat
      361 ataaaccccc agtgccataa ttgataaaat taaactttgc gtcattacag atataatacc
      421 tgacataaat aaaacactcc tttgaaaata tttttataat tttaattatt tgctaatttt
      481 gtgtataaaa aaaccacata gtgtacaaaa ctatgcggta aaaacacgaa taagccacat
      541 agtttaatct atgtggctct tatatagtta aatgaaaact ttataaacaa tatagccaac
      601 atagatacaa tcccaaaatt ttagtagtct taaggggtct gtatctatgt taaaacgtag
      661 gtacaaaccc taaaaatagt ggctatagta ataataatat tttttattgt ttaataaagt
      721 attcataatt atctctccct tttaatattt taattagtca gaaaattata tgtattctcg
      781 acttgttata aattataaat tatacaataa taaaagtcaa ctgctaaatt taattaaaat
      841 actaaataat gtatactcag aatattttta aattaatgta ttatttgcaa aaaacgttat
      901 cctaagttat aagtgaatgt ggaaatttta tcgaaatatt tactaaatat ggaataacta
      961 gacacatata tcaatcaaat ctcataaaaa aaagctgtct agagaatatt aaaattcttt
     1021 tggacagctc aagtagtgat tagaaagtta tataactttc ataatcagtc gtcaatatag
     1081 acgactttta tggaaaatga ataactttat atgcaatatt caaattgcta caataagcac
     1141 aattttcaaa gggttagggt ggataagagt acttattata taaagttttc atattaccta
     1201 cttcatatta cctatttttc gaaattaata agttttattt tgcagatttt gcattttcat
     1261 ctgactttat atactttata ttatctgctg ataacttttg catgtcaagt gcttttagtg
     1321 tatcttcgtt aattattatt tcagtttcat ctaatgtagt aactggtata tcgccaacag
     1381 attttccttc taatacttca atagcaagtt ctccagcctt ataacctagt ttttcataat
     1441 ctattccaca acaagctaaa gcaccagaag atacagaacc ttcttcagag gctattatag
     1501 gaatcttgtt ttcattagca actttagaaa ctattggcat agaagaaact attaaattgt
     1561 cagtagggac atataaaaca tctattttgc caactaaact agaaatagct tggttaactt
     1621 cacttgaaga gctgattcct ttttcaacta cttcataatt atttttctta gcataatcat
     1681 gtagagaatc aacttggatt tttgaattaa cttcactagt attgtatata acccctattt
     1741 tctttgcttt tggagtcaat gttttaacca attctaatgt tttatcaatt gaaagatagt
     1801 cagatgtacc agaaacattt ccacctggtt tttcaagaga tttaacaagt ccagcttcta
     1861 cagggtctgt aacagcagtc attattatcg gaatatcttt agtagcatta taagctgcct
     1921 gtgcagatgg agtagatata gcatatatca aatctttttt gtcggataca aacttactag
     1981 caatactttg tgtagtaggc atatcatttt gtgcattttg gaaatctatt tttatattat
     2041 ctccatcttt atagccttta tcttcgagtg ctttgatgaa tcctttcttt gctttatcca
     2101 gagatggatg ttccactaat tgagtaatac ctatattttt ttgttttttg ctatctgttt
     2161 ttttattttc atttgaagca ttggagccat cattttgaga acatccagta agcattgata
     2221 caccaagtac tcctgctata attaaactta atattttttt actttttatc attttatttt
     2281 cctccctcaa aatttaataa ttatatttgt tcatatattc atataataga ttataaatat
     2341 ttctgattaa aactaagatt ttaatttcct atactgcata ttcaaatttt tctaagattt
     2401 cttcaactgt taaatcttct ttttctttat cttttacatc caaaacaact tcacccttat
     2461 gaagcattat caacctatct ccatactgaa gagcgtgttt caagttgtga gtaaccataa
     2521 gtgtagttat atttttttct cttacgattt tgtctgttaa ccctattact tcatttgatg
     2581 tttttgggtc aagtgctgct gtatgttcgt ctagtaataa gaccttagga cttgctaaac
     2641 tagacataat tagtgatagt gattgtcttt gacctcctga aagatattgt actttaatat
     2701 ctaaatattt ttttaaatcc aaagaaattc cttcaagtaa gtgttccaaa taattcgttt
     2761 tatgacgaag acattttttt aagttcaata atttcccttt attaagtgct agagataaat
     2821 tttctctaac cgtcatagaa ggacaagttc caaggcttgg gtcttgaaaa actctactta
     2881 ctatttgagt ccttttatac tcagctaaat ttgttatggt agtactatct aaagtaattt
     2941 gaccagaaga ttcttttata agcccagata ttatatttaa taaagtagat ttaccagttc
     3001 cattactacc tatgatactt acaaattcac catcttctat atcgattgat aaattttcaa
     3061 atatagt
//
LOCUS       DAEMAE010000313         3044 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEMAE010000313 DAEMAE010000000
VERSION     DAEMAE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.318"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..957
                     /locus_tag="KPH93_003534"
     CDS             163..957
                     /locus_tag="KPH93_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690301.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEEIEESIGNVVNIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEEI"
     gene            958..3018
                     /locus_tag="KPH93_003535"
     CDS             958..3018
                     /locus_tag="KPH93_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1690302.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSTNYQIYINSSQENSSNAVKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMTGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLVIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFVRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILVMLGLRVLDRK
                     SLRN"
BASE COUNT         1167 a          310 c          506 g         1061 t
ORIGIN      
        1 tacaaaagat attataaatg aataaaaaat aataatttgt cagataaact tgtaaattat
       61 tatgaataat ctattctaaa acaaaaaact gtgtataaaa aaggtttata tgttatattt
      121 atataaatat aacatatgaa tgagaaagga gtagtttgat aaatgattga ggtagataaa
      181 ttatgcaagt cttttacaag ggtcgtaaaa gatgataaga ataaaagttc aataagaaag
      241 ttaaagaaag taaaaactaa aaaagaagaa ttttttgctg taaataatgt ttcatttaaa
      301 gttatggaag gtgaaattgt tggaatatta ggtccaaatg gggcaggaaa gactacatta
      361 cttagaatgt taggtggcat attaacacca acatcaggaa gtataaatat ttctggatat
      421 gattattcaa tagatagaaa ttcagcaaaa aaagaaatag ggtatttgtc tggaaataca
      481 aaattatatg gaaggctttc tcctagagaa cttctaacaa cttttgcaag tctatatgaa
      541 atgtcaaaag aggagataga agaatcgatt ggaaatgttg taaacataat ggatatgagt
      601 gaatttatag ataatagaat tgaaaatcta tcaactggtc aaactcaaag aacatctata
      661 gctcgttgtt taatacattc tcctaaagta tacatttttg atgagccaac actaggtctg
      721 gatgttttaa gtagtgcatc aattatagat tttatgaaga atgaaaaact aaggggaaaa
      781 actgttcttt actcaaccca ttatatggaa gaagcagaaa ctttatgtga caagattttt
      841 atgattcaca atgggaaaat aattgcatca ggaacaccac agagtttaaa agaggaatcc
      901 ggagtaaata atttgagaga tgtattcata aagcttgcca gaaaggagga gatttaaatg
      961 agaagtaaga ttgtaaaata catatttaaa aaagaaatgt tagacatatt gagagataaa
     1021 aaaactttat ttatgatgat tgttgtgcca cttcttttat atcctttact tatgttatta
     1081 atgattcaag taatgaatat gtctgcaagt agtatgaata gcaaggacat taatatagct
     1141 tttaataata aacccaataa ggtgttagta tccatgattg aagatgataa tttagaaaaa
     1201 gatactaaaa atacttcaaa tgataaaggc aaaataagag tgttagatgt agataactat
     1261 aaaaagtcat tagaagaaaa taaaattgat gcttatataa agatggaaga aaaaaaatca
     1321 tctacaaatt atcaaatata tataaattct tctcaagaaa attcttctaa tgcagtaaaa
     1381 agaattgaaa ctgttttgga taaatataaa agatttacca tagaaaaaac tttatctgaa
     1441 aaaggattag atgtacaagc tactttagaa ccaataacat atagcactgt agatgttgca
     1501 aagacggaag aagtggcagg atacttttta ggtcagatac ttccatttat attaataata
     1561 ggagttttac taggttcaat atatccagca atagatgtta tggctggaga aaaggaaaga
     1621 ggtacattag aaacattatt tacactccca atatcaaact tagaactggt tatgggaaaa
     1681 tatatggcag tttctcttag tgcaatagta acagcaattt taaacatttt atctatggga
     1741 ctgactatgg catttatttt agttagtgga ggtattagta gccaatttgg ctttggaaac
     1801 tttaattatg gagacttagt cttaccaatt ataactactt taatttgtat atgtcttttt
     1861 tcaatggtag tttcagcaat aagtatgtgt gtatgttcat tagcaaaaag ttttaaagat
     1921 gctcaaaact acataacacc tcttatgtta attattatga taccatcata tgtttctatg
     1981 ataccaaata tagtattaga tagagtaaca gctgtaatac ctgtagtaaa tatatcttta
     2041 cttataaaaa gtgtattatc ttttaaaagc gatttaaatt taatagcctt agttttagca
     2101 tcaaatgtag cctttgttat attggcaatc atactactgt ctaagatgtt taattctgag
     2161 gaaatacttt ttggtaatag taagagtttc tctttcttag agaaaagaag taatataaaa
     2221 aaagggtcta taccaactgt aagtgatggt gttattttat atgcagtagg attagttcta
     2281 ttgatttatg ttggttcttt agttcaaatt aaatttggta tgacaggtat tataatgaca
     2341 caattcatga taatttcttt gccgttatta tttgcatact atattaaagc tgattttaaa
     2401 gaggtattta gcttgaaagt tcctaaatta aagcacgtgt ttgggagcat tgttctttgg
     2461 attggtggat atatacttat tatgattata acacagctaa ttttattctt atttcctcaa
     2521 aatatggaag ttgctgaaag tttaaataag gctcttttta tcaaagatag tgttttattg
     2581 aatttactag taatagcttt attaccagct atatgtgaag aaatgttttt taggggattt
     2641 atattttctt catttagtaa atcaaaagat aaaaataagt ctatcaagct tgctataata
     2701 tgtagtgggg tactgtttgg tattatgcat atggattttg ttagaataat tccaacatca
     2761 atattaggta ttatatttgc atacagtgtg tataaatctg gttcaatatt tgtatcaatg
     2821 ttattgcatt ttttaaataa tagtgtagca gttgtgttaa atcattatac aactggaaat
     2881 ataaccaact tatataaatt tgttgaagtt gatttttcga acttaaatgt acttaaattt
     2941 gctttaattc ttctaataag tcttatatta gttatgttag gattaagagt tttagataga
     3001 aaatctttaa gaaattaagt ataaagttat tatatatatt aaaa
//
LOCUS       DAEMAE010000314         3031 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEMAE010000314 DAEMAE010000000
VERSION     DAEMAE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.319"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..365)
                     /locus_tag="KPH93_003536"
     CDS             complement(<1..365)
                     /locus_tag="KPH93_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1690303.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEG"
     gene            658..867
                     /locus_tag="KPH93_003537"
     CDS             658..867
                     /locus_tag="KPH93_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690304.1"
                     /translation="MKSIRVYKNMNIRRCNFTILKKVNAINYLYKVDYKQKISVIVNT
                     VKDEGGVLIYIYMGRNFDYNEKTYS"
     gene            909..1271
                     /locus_tag="KPH93_003538"
     CDS             909..1271
                     /locus_tag="KPH93_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690305.1"
                     /translation="MKIRKSGFISIECIISIAILYVVVYLVSTSLYNCYSFISRSMSD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIKQILDYYQCYEINI
                     EIKNEFKELRFNSYVTRK"
     gene            1255..1971
                     /locus_tag="KPH93_003539"
     CDS             1255..1971
                     /locus_tag="KPH93_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690306.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIAVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKEIKVRVKIKNPWVGIDIEDYMDK
                     SNNNKIDDKTFNNSKVSENNEILNQFKNESENISNQEGVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            1991..2410
                     /locus_tag="KPH93_003540"
     CDS             1991..2410
                     /locus_tag="KPH93_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690307.1"
                     /translation="MTVIELIVSISIMLLIVTLSFPKDNLENHKINLFAHQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2400..2924
                     /locus_tag="KPH93_003541"
     CDS             2400..2924
                     /locus_tag="KPH93_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690308.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIMLVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEENISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESV"
BASE COUNT         1266 a          251 c          483 g         1031 t
ORIGIN      
        1 ttaccttctt tatctgttcc gatagggtct tgtagagata cttgagtttt attttttttg
       61 ttagatctaa tattcattaa aatctcattt tcaatacatc tagaagcata tgttgctaat
      121 cgagttcctt tatctatgtt ataagtttct atagctttaa ttaatcctat tgtacctatg
      181 gatattaaat catcctggtc ttccgttgaa ttattatatt tctttgctat ataagctact
      241 aatctcatgt ttctttcaat taatgtatct tttgcacttt tatcgttttc tattttaaat
      301 ttagttaaat actcaatctc ctcttcaggg gttaagggtt tttcaaaaga tttaagagct
      361 gccaataggt cacccccttt tcaacggtca ctttaaatta tatgaaggag acttatccaa
      421 gttgactgtt atctaataaa ataatatgca tatttttaga atttaaaaca tatatttgca
      481 aacttttgaa tggaatatga cataaagagt gggaatttaa atttaagttt ttgttgtata
      541 tatatgaaac aaggcgaaga aattattgat ttataaagta taaattggaa ttgaggtgtc
      601 aataaatata aaaggaaata gtatttatat atgttttgat gttgaggtaa aagattcatg
      661 aaaagcatta gagtctataa aaatatgaat attaggaggt gtaattttac aatacttaaa
      721 aaagtaaatg caattaatta tttgtataaa gttgattaca agcaaaaaat atcagttata
      781 gttaatactg ttaaggatga aggtggtgtg ttaatttata tatatatggg aagaaatttt
      841 gattacaatg aaaaaactta tagttaaatg tttatatata aaatattatt taggggttgt
      901 ttttattatt gaaaataaga aagagtggtt ttatatcaat cgaatgtata ataagtattg
      961 ctatattata tgtggttgtt tatttagttt caacatcatt gtataattgt tatagtttta
     1021 tcagtagaag tatgtctgac agagaaatgt taagtacagc aaaaaaatat atagaagatg
     1081 agaagtatag aatacaaaat agtaagtatg agttaattga agataagata gaaaaaaatt
     1141 acataaatgg atatgaaatt aacagtagaa taaagcaaat tttagattat tatcaatgct
     1201 atgaaataaa tatagagata aaaaatgaat ttaaagaact gaggtttaat agctatgtta
     1261 ctagaaaata aaggctctat attaataata acattgataa tattttctat tataagtact
     1321 gtgtgcatta tgtgtatagg tttaatttat acacataata atatgtttga gttaggatac
     1381 aaggacattc aactaaaaga aatttcttta agtggaatag aaatatcagt aagtaatgta
     1441 ctttcttgtg ttgaggaagc tattgctgta tctaataatg aagacgaatt tagagaatat
     1501 ttcttaggaa ataacatgat tgaatgtata aaaaaaataa aagatacatc ttatagtagt
     1561 ttagaagaac tttctttaaa agtagaaaat aatactattt atgatagagg agattttttt
     1621 gaatttgata tagaaagtaa tgttagagaa aaagaatttt taaaagaaat aaaagtaaga
     1681 gtgaaaatta aaaatccatg ggtaggaatt gacattgaag attatatgga taaatcgaat
     1741 aacaataaaa ttgatgacaa aacattcaat aattctaaag tgtctgaaaa taatgaaatt
     1801 ctaaatcaat ttaaaaatga aagtgaaaat ataagtaatc aagagggtgt tgaatttaca
     1861 gaagaagatg aagaatatga agaagaactt aaacaaaata taattgaaaa tataaagaag
     1921 aattttgatg aaaaaaaact agtacaggta tataagtata ggggtgtata actttgaaaa
     1981 aagaaggaat gtgactgtaa tagaattaat agtatctatc agtataatgc tcttgatagt
     2041 tacattatct tttccaaaag ataatcttga aaatcataaa ataaatttat ttgcacatca
     2101 actttgttca gatataaggt atgttaggcg aattaatatg tttagcaact acaatacata
     2161 tatatattat ataaatcaag gtaaacacga aggttatgta ttgagaaaag atggaaaaaa
     2221 cattaaatca ataatgctac ctaaaaatgc aaaaatagct agaagtattg aaataataaa
     2281 gtttaaatct gatgggtcgc ctcaaaaggc gtctacaata gagatatata ataatagatt
     2341 aagaaaaact ataacaataa cacctgtaag tggaagagta cttctgaaag agggaaaata
     2401 tgaaacataa atatggttac ttgcttttgg aaagtgtagt atcattgtct tcaatggtga
     2461 taattatgct agttttatat tctatatttt tgtctacaat aaacttaaag ttaaaagtag
     2521 aagataagat agaactacaa caacaaagtc ttgaaatcat aaaatctatg gaaggaatta
     2581 ttagcaattc tatgggaata atgaatgtat ctaattatga agaaacattt aaaaagacaa
     2641 catctataaa atgtagatat gtagatgaaa ataataatga agaaaacata agtaataaag
     2701 aaattatatt gaatgaaaga cgaaataagt tatttgtaaa ttctttaaat ggcgaaagta
     2761 gtcaagcagg tggctatgaa attggagatt atgtagatga gatgtatgta tcgattacta
     2821 ataatggtca atatgttaat attaaattga aattatcaaa acgtagtcaa aaatatgaga
     2881 ctgatttcaa aataaaggta tggaatttta gtgaaagtgt ataaaaatac ataaatttaa
     2941 gagaaagaaa tgacatcaat aagtcatata aaatataatt attgagaact atataactaa
     3001 ataatatcaa tatgtaaagt gtatagatta a
//
LOCUS       DAEMAE010000315         2982 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEMAE010000315 DAEMAE010000000
VERSION     DAEMAE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.320"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   154..573
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=221..249
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1218..2489
                     /locus_tag="KPH93_003542"
     CDS             1218..2489
                     /locus_tag="KPH93_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1690309.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRNLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1166 a          375 c          388 g         1053 t
ORIGIN      
        1 tttttataat tattctactt ttatatttta atcattaaaa ctaaagtcaa tgttatctgg
       61 taattattta ctacaaatga taaaaaataa ttatatttta ggggagaaaa tgctatataa
      121 attattgggg tattttacct atacttgttg caaatttaca taccacgtag ttaatataaa
      181 acgtggtggt gatatgggag gtgtaattaa aggttataaa atttacatac cacatagtta
      241 atataaaaca gaacttctac tcctcgacca ccaccactgt tgaaaattta cataccacat
      301 agttaatata aaacgaatta gaatctggtg cagttgatga agtcggtcaa atttacatac
      361 cacatagtta atataaaacc aagttacagt atttaatttc atagataagt tattatttac
      421 ataccacata gttaatataa aacggagtat ttcataacag attttgaaac agatatagaa
      481 tttacatacc acatagttaa tataaaacaa taaatatatg tgacctttta gaaggtaaaa
      541 caatatttac ataccacata gttaatataa aaccccaaaa taaactaagt attttcagta
      601 cttacacaca tacaactctc tcaaatttgc agtgaaccat gagtagtgca attgataaca
      661 tttatcatac accctcaatc ccttacattg caactgttaa accctacttt atcaaaaata
      721 tcgctcactg caaaatttat atatttttat tatatcataa aattcaaact ataaaaataa
      781 aatggaataa tttatcaatt taagaacacc tgcatatctg taaatactta ctatattatc
      841 tattctaata gttcttcttc tagctggaac taatataaaa atcatctctg aaagattagg
      901 acatactgat ataaaaataa ctatgaatag atattttctt attttatatg atatggataa
      961 agaagcttct gaaaatataa tcaaggttat ctttaaataa tgttcagttt tatgtcagta
     1021 agtatcgtat ttttcttaaa tgtcacttag atattcagtt atatataata gtaaacaact
     1081 gtatttcact aatatttgct attatctaat agtatttata gtttagattt gatataatac
     1141 atgtggtaaa attttgattt gtaaaacaaa atataaccta catatattaa aacaaattta
     1201 aaactaagga gaactatatg ttaaaagtac taaaaggaaa tataattttt acaaaaactt
     1261 cagaaacttt cacagtattt gaaaatagtt atattatact ggaaaaaggc aaagtaaaag
     1321 gaatatttaa agagatacct aaggaataca aaaacctaaa agtagtagat tacactgata
     1381 agcttataat acctggtatg aatgacctcc atgcccatgc ctctcaattt aaaaatcttg
     1441 gaatggctat ggataaagag cttttgccat ggcttgaaac ttatacattt ccagaagaat
     1501 caaattataa agatatagaa tatgcaacaa aaatgtataa aaaatttgta aaagaacttt
     1561 ggaaatctgg aactacaaga atagcagtgt ttgcaacagt acataaagaa gcctctatga
     1621 aacttatgga tatattcaaa gaagttggtc ttggagcata cgttggcaaa gtaaatatga
     1681 atatgaattg ccctgatact ttattagaaa acactcaaga ttctattgct gatacagaag
     1741 aaattataaa taagtatagt gatacagatt ctattgtaaa accaataata acacctagat
     1801 ttataccaag ctgtacaagt gaacttttaa aaggtttagg agatttatgt ttaaaatata
     1861 atattcctat acaatcacac ttatctgaaa attatgatga gattgaatgg gttagaaatc
     1921 tagaacctga atctaaattc tatggagatg catataatag atataattta tttggtcaaa
     1981 ctcctacttt aatggctcat tgtgttcatt gttctcagga tgaaatagat ttaatgagag
     2041 aaaataatgt tatggcagtt cactgtccag cttctaactt taatgttggt agtggtgcta
     2101 tgcctatcag aaaattaatc gataataata ttagattagc acttggctca gatataagcg
     2161 gtggacatac attgtcaata tttaaagcaa tggtatctgc aatacaatta tctaaattat
     2221 attgggttaa ttctggtaag aagtataatt tcttatcttt atctgaagct ttttatattg
     2281 caaccaaaag tggtggaagt ttctttggaa aagttggtag ctttgaggaa ggttatgatt
     2341 ttgatgcatt gattattgat gattcaaatt taaatcatga caattattct atacttgaaa
     2401 gacttgaaag atttatctat gttggtgatg atagaaatat cattcataga tatgtatgtg
     2461 gaaaactaat tgaagaacct aatatataat tataataaaa attttaattg tataatcttg
     2521 ttaatttata caactataat taataaaaga gtatatttta aatctaaaag ctataggttg
     2581 aaacatactc tttttatttt gtttatttat agctttagtg attaatcata tataaatcta
     2641 caaaatgtat tcctagtaac ttacattttt tgatatccac aatcataaaa ctatattatt
     2701 attggattca tacctcaaaa atatcgcctt ctggacacta ttatattcaa agctatgtta
     2761 atttgcaatg atatatctaa catgtattga ttaaaaaaat taaaacttgc tttttatctc
     2821 ctatatctaa agaagtggca tttagaataa actattgtaa tattaaagtt gaaattttct
     2881 agtttattta ctctatagca ccatattata atactattaa ataaaaatgt agttaaaata
     2941 tatactaata tccttgaaat aagtatttct tttttattta gc
//
LOCUS       DAEMAE010000316         2972 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEMAE010000316 DAEMAE010000000
VERSION     DAEMAE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.321"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..852
                     /locus_tag="KPH93_003543"
     CDS             136..852
                     /locus_tag="KPH93_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG1690310.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRKFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            910..1488
                     /locus_tag="KPH93_003544"
     CDS             910..1488
                     /locus_tag="KPH93_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1690311.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYIDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            1882..2754
                     /locus_tag="KPH93_003545"
     CDS             1882..2754
                     /locus_tag="KPH93_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1690312.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         1295 a          290 c          506 g          881 t
ORIGIN      
        1 ttacaattat ttaggaaaat taaataattt ttttgcttgt ttttaattaa ggtctagtat
       61 agtataataa tgtagtaaaa ttaacattaa tagaaatatt aaacaacaca taattaagat
      121 aaggaaggat taagtatgag aattaataag tatatagctt cttgtggaat agcgtccaga
      181 agaaaagcag aagaaataat tcttggagga agaataaagg taaatggaag tgtagtaaaa
      241 gagctatctt ttaatattga tgaagaaaaa gatatagtag aatttgataa taaaaaagta
      301 aaaccaagtg aaaattatat atatatagta ttaaataaac cagagggata tataactaca
      361 gtaaaagacc aattcaatag acccagtgta atagatatac ttaaagatgt aagggaaaga
      421 gtatatccaa tagggagatt agattatgaa actagtggat tattaatact tactaatgat
      481 ggtgatttaa catataagct tacccatcca aagcatgaaa ttgataaaac gtatgtggca
      541 agtgtgaagg gaattataag tgatgatgag ataagaaaat tcgaaacagg actaaaaata
      601 gaagattaca caacagcacc tgccaagata agggtaacta aagaaaataa agaaaaaaat
      661 tattcagtgt gtgaaataac tatacatgag ggtagaaata gacaagtaag aaaaatgtgt
      721 aaagctataa atcatccagt tttaaattta agaaggattt ctgtagggaa aatagtatta
      781 aaagacacca aagttggaga atatagatac ctaacagaag atgaaataaa atatttaaaa
      841 tcaataaaat aagataaaaa taaatggaga aaaaatgaac taaagttaat agtagggaga
      901 aatagtatta tggcatatgc aaagtattta actaaaatta cagatataaa taaattttat
      961 ttagaaaata ttataaatga aggtgacata gtaattgatg ctactatggg aaatgggtat
     1021 gatacaaggt atctagctga gaaggttgga gaaaagggct ttgtatatgc ttttgatata
     1081 caagaagaag caataaagtc tacaagaaag aaactagaga aagaaggata tatagataga
     1141 gtaaaattga tatttgatgg gcatgaaaaa atgaatcaat atataaaaga agaagtttca
     1201 tgtgttttat ttaatttggg ttatcttcca agagcgaaac attcaattat aaccaaacca
     1261 gatacaactt taaaggctat aaagttaagc ttagaaatgt taaaagaaaa tggagtaata
     1321 agcattgcca tatatacagg gcatgatgga ggtcaagaag aaaaagattg catatataac
     1381 cttgtcaata atttaaatca agatgaattt aatgttttag aatctaagtt tttaaatcaa
     1441 gtcaataacc caccacaatt gattttaatt gaaaagaaaa aagcatagta aatactaaaa
     1501 aacttgacaa gattctatta agagttaata tatatagtgt gacctattaa aaatacttta
     1561 aataataagt atacaacaac ttatcctcaa aacaaaatat acatctaaat atgcaaaata
     1621 agatagataa cttttaaatg agtaaaaatt gaaaaattta aaagaaatat tggataaata
     1681 ttaaaaaatg caaggaaaga tggtataata aatttaatcg aaaatactat gtgaagtata
     1741 aggtacagaa aagttagaat tttatgagtt taagaagcta tgataaaaac taagatgaaa
     1801 ttgagataaa aaataaaatt tttagagaaa aagacagtgt taataaaaca aaatagttta
     1861 tataactaaa aggagaaaaa aatggaagaa acaaatgaaa tgaaggacag taaacatttg
     1921 atatcaaata tacaatctca atatacgaga ttgagtaaag gtcaaaaact tatagcacaa
     1981 tacattttaa ataattatga taaagttgca tttatgactg catgtaaatt gggtgaaact
     2041 gtaggagtaa gtgagtctac agttgtaaga ttcgcaaatg cattaggtta tagtggatat
     2101 cctaaattac aagctgcact tcaagaactc ataaaaaata aactaactac agtacaaaga
     2161 gttgaaatgg cacatgatta ttccgatgat tttgcaatac taaacaaagt tttaaaaagt
     2221 gatatagata atataaggtc tacactagaa gaaattgacg agagagcgtt taaagaagct
     2281 tctaataaac ttttaagagc gagaaaaata tatatacttg gaatgaggag ttcttttgta
     2341 gtagctcaat atttaggttt ttacttagat ataatacttg acaatgttca tataattaga
     2401 atggatatgg gagatgcttt tgagcagatt gtaagaataa atgaagaaga tgtaatagtt
     2461 gctattagtt tccctcgtta ttctaagaaa tcttatcaaa ttgtcaatta tgctaaggaa
     2521 aaaggagccc atgtaatatc attaacagat agcttatttg cgccagtagc atcacttgca
     2581 gataacactc ttctagttaa gagtaatatg gcttcatttg tagattctct agttccagct
     2641 cttagtattt caaatgccct tgctatatct gttgggatga aggaaaaaga agatattaaa
     2701 cagcattttg atgatttaga gcaaatttgg aaaagatatt ctgtgtatga gtaaagtttg
     2761 tatattaata aggctatgga attgagaatc atagccttat tagtaaattc ttgattatca
     2821 cttaaaaaat aaattttcgt ttaaataata caaataaata tttcgagact caaaacataa
     2881 agatgtattc tgaatacatc tttactacgt caactgattt attaaagaaa gcatttccct
     2941 tcatggagag tgcttttttt atttctctaa ac
//
LOCUS       DAEMAE010000317         2959 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEMAE010000317 DAEMAE010000000
VERSION     DAEMAE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.322"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1373
                     /locus_tag="KPH93_003546"
     CDS             <1..1373
                     /locus_tag="KPH93_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG1690313.1"
                     /translation="VRTCLYFVMFSIICILINILVSVLIVGFNSRSSKYVLSLNGKKS
                     YGFVNIMHFTLKIVFVSFLILSINNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTG
                     EQSLGKEEVIKNAKIKSFYKSLVEEKDAFLIDTTNFEKLEDGSYMYEINSKGKNPEVS
                     QYGKNIKINKNYLKVNPIYSNGKEIFNLIDYNDKTINLLVPKKLQVHENDIKKEFREL
                     FYFEKIEVENMYNEKLNRGLNKTKESDLNVNIIYVDNNQSYFTYNSFVMEDNRNLIDD
                     PIVIIDIDNIDDSFYLSYISRCVYFNSKKLDALADISNIIESQGVEAYIQSLHAVYNE
                     YGLEINKLEKYLNSEIFTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSTTAR
                     SMLLIVSLILTNIIPISIISTRVDLSNNIILFGFLILVVEVIISIILDKIISNSSFNK
                     IIKGEH"
     gene            1378..1995
                     /locus_tag="KPH93_003547"
     CDS             1378..1995
                     /locus_tag="KPH93_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690314.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAIIGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVSLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            2184..2528
                     /locus_tag="KPH93_003548"
     CDS             2184..2528
                     /locus_tag="KPH93_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG1690315.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDANGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT         1247 a          272 c          420 g         1020 t
ORIGIN      
        1 gtgttagaac atgcttatac tttgttatgt tttcgattat atgtattttg ataaatattt
       61 tggtatctgt attaatagtt ggttttaata gtagaagttc taaatatgta ctatctctaa
      121 atggaaaaaa atcttatggt tttgtaaata taatgcattt cacattgaaa attgtatttg
      181 tatcatttct tatattatcc ataaataact gtattttaaa ttataatatg ctacaaactc
      241 agttagggaa tctatctgag tgggataaag caaaaaatgt ttataatctt actcttaaag
      301 atacaggcga acaatcttta ggaaaggaag aagtaattaa aaatgctaag attaagagtt
      361 tctataaaag tttagtagaa gaaaaagatg ctttcttaat tgatacaact aattttgaaa
      421 aacttgaaga tggaagttac atgtatgaaa taaattcaaa gggtaaaaac ccagaagtaa
      481 gtcaatatgg taaaaatata aaaataaata aaaactatct taaagttaac cctatttact
      541 caaatggcaa agaaatattt aatctaatcg attataacga taagactata aatttattag
      601 ttcctaaaaa attgcaagta catgaaaatg atattaaaaa agaatttaga gaattatttt
      661 actttgaaaa aatagaagta gaaaatatgt ataatgagaa gctaaataga ggtttaaata
      721 aaacaaagga atcagattta aatgttaaca taatatatgt agataataat caaagttatt
      781 ttacttataa ctcatttgtt atggaggata atagaaattt aattgacgac ccaatagtaa
      841 taattgacat agataatatt gatgattctt tttatctttc ttatatatct agatgtgtat
      901 attttaattc taagaaatta gatgcccttg cagatataag taacattatt gaatctcaag
      961 gtgtagaagc gtatatccaa agtttacatg cggtttataa tgagtatggt ttagaaataa
     1021 ataaactaga aaaatatttg aatagtgaga tttttactat aataataata gcaatatcaa
     1081 acttaatgat tacttacaat atagtagcta gctactatga aagaaataaa tataagttat
     1141 atattaaaaa aatatttggt tattcaacta cagctagaag tatgttgtta atagtctcat
     1201 taatattaac aaatattatt ccaataagca ttatatcaac aagagtagat ttaagtaaca
     1261 atattatctt atttggattt ctgatattag ttgtagaagt aattatatct attatattag
     1321 acaagattat ctcaaatagt tcatttaata aaattataaa aggagaacat taaaaagatg
     1381 atttcattaa taaatatatc gaaaaaattt aaagataaat ctataataga caaatttaac
     1441 ctagaaattt ctaagggtga gtttgtagcg ataataggaa atagtggttc aggtaagaca
     1501 actttactta atataatggg attattagaa aaacctaatt ctggtgatat tataataaat
     1561 aatataaaaa atccaaatag taaacagata agaattttac aaagggattt ttatggttat
     1621 ctatttcaaa attatgcttt aattgagaat gaaaatgttg aaaataactt gaaaattgct
     1681 ttaaagtatc aaaaaaatat caaccataat gaaaaaataa attacgcact tgagtctgta
     1741 agtcttagaa aatacaataa aaaaaagata tacgaattga gtggaggaga acaacaaagg
     1801 gtagcattag caagagtaat attaaaagaa tgtgaagtaa tatttgcaga tgaacctaca
     1861 ggcaatttag ataaaaaaaa tagagacata gtatttgata tacttaaaag tctgaataag
     1921 aatgggaaaa ctatcgtatt tgtaactcat gatttagagt tatcggagca ggcagataga
     1981 gttataaaat tgtaataata tcaactataa taaatataag aaagagatta cagctaaatc
     2041 aagtttaata atactacgaa tagaatttaa acattaagta aattagtagt ttgtaaatat
     2101 aagtataata tgatatcata aaggctcaaa agaaatttta tataataaga aatctaaaaa
     2161 taacaaatgg aggacatata aagatggaaa atttaccaaa ttgcccgaaa tgtaattcag
     2221 aatatactta tgaagatgga actcttttag tttgtccaga atgctctcat gaatgggcat
     2281 taggattggg agctgaagaa gattcaaata ttatcaaaga tgcaaatgga aatgtattaa
     2341 atgatggaga ttctgttaca gtaattagag accttaaagt aaaaggaagt tcatcatcta
     2401 taaagattgg aacaaaagtg aaaaatatcc gtttaatcca tgactcatca gatggacatg
     2461 atatagaatg taagatagat ggttttggag ctatgaagct aaagtcttct gttgttaaaa
     2521 aagcgtaata ttataaataa gctgtagtgt ttattgtttt tcagtacagt ataaaaccat
     2581 aagaacctat taagtaataa aagtttgttt atttatcttt atattttctt atggttttat
     2641 taaatataga ttttattgaa caccaaaata ttctctcctt tacaaataag ccaatcttat
     2701 ttaaactaat atactttata ctatttatac tgaaattgta aatttaaatg ctatgttatt
     2761 taggtttata tagttaattt atatatttct gattttataa attgtattta ttattatatc
     2821 ttaattctaa aatcattaaa aatttcaatc tctataaaga tttacaattt ctcaaataaa
     2881 ttattagtat ataaacaatt tattattaca gtgctgcatt taaaataagt cttgatataa
     2941 aggtattgtt acaagtata
//
LOCUS       DAEMAE010000318         2925 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEMAE010000318 DAEMAE010000000
VERSION     DAEMAE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.323"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..1114
                     /locus_tag="KPH93_003549"
     CDS             191..1114
                     /locus_tag="KPH93_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG1690316.1"
                     /translation="MGSGKVGTSLANYFLSKGHCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVVQNFIDGLGNHFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEDESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIKKHLSVLDKKDEEIYKILSLNLLKIIALGNDNSLKCTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTNF"
     gene            1089..1916
                     /gene="panB"
                     /locus_tag="KPH93_003550"
     CDS             1089..1916
                     /gene="panB"
                     /locus_tag="KPH93_003550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG1690317.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIENIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     AKQLIEDAKLIEKAGAFSIVLEGVPAKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVKTGAFPQEKHSFSINESELEKLY"
     gene            1935..2783
                     /locus_tag="KPH93_003551"
     CDS             1935..2783
                     /locus_tag="KPH93_003551"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG1690318.1"
                     /translation="MLVKEIKLLRNIIKDWRKDGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELIIAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1177 a          348 c          469 g          931 t
ORIGIN      
        1 aaattttata gaattaattg tcaatttttt atgaaaatgg taatatattt atggatggta
       61 tcttgttata agaactatac tttaaatgtt tttaattatt ttgtcttatt taactcatat
      121 cctcaggaat gagattagaa attaagatgt tgtatcacaa agtggaggtg atactattaa
      181 aataggcttt attggttctg gaaaagttgg aacatctttg gcaaattact ttttatcaaa
      241 aggtcattgt gtttcaggct tttatggaag gaatcagtta tctttattag aatcaacaaa
      301 tttaacaaaa acaaagattt acagcaattt gaaagatata atttatgaaa acgatatatt
      361 atttattaca acatcagatg attctattga aattatagac aaaaaacttt ccaaatttaa
      421 tttaaaacat aaatacattt gccacacaag tggctcttta aaatctagta tattgttctg
      481 ttcaaaaaaa gcaggtgcat taatttattc tatccatcct atatttgcat tttccaataa
      541 aaataccgac ataaaaaaaa tgaaagacat ttgtttttcc atagaaggtg ataatgaaca
      601 agatgattta gttgttcaaa atttcataga tggtttggga aaccattttt ttataagaga
      661 taaggatact tcatccactt atcatttagc taatgtttta gtttcaaatt tagtattatc
      721 tttactagat ataggagtaa gttactttat taaattaggt ttatctgaag atgaatcttt
      781 aaaagctatc agtcctttag taaaaaaaaa tatagaaaat atacttgata atggctttac
      841 taaagctctt actggacctg tttctcgtgg agatataaca ccaataaaga aacatctatc
      901 cgtattggat aaaaaagatg aggagattta caaaatactc tccttaaacc tactaaaaat
      961 aattgcctta ggaaatgaca attcattaaa atgtacaaaa aatattacta tagaaaatgc
     1021 aatcgaaaat ttaataagta aatctgaaaa atacaaggaa atttacaaac ttttaggagg
     1081 aaaaaacgat gaaaaatacc atacaaactt ttaagaatgc taaatatgaa ggaaaaaaac
     1141 tatctatgct tactgcttat gactactcaa ttgcaaaaat aatggatgag tgtgatataa
     1201 atggtatttt aataggagat tcattaggaa tggtcataaa aggtgaagaa aacactctat
     1261 ctgttactat agacgaaatt atatatcata caaaagctgt taaaaatggt gtaaaaaatg
     1321 ccttaatcgt aagtgatatg ccttttctat cttaccatgt ttctatagaa gatgcagtta
     1381 aaaatgctgg tagacttata aaagaaggag gagctcatgc tgtaaagtta gaaggtggct
     1441 caaacgttat aaaacaaata gaaaacattg taaatgctca gatacctgta atggggcatt
     1501 taggattaac tcctcaatca gtaaattcat ttggtgggtt taaggttcaa ggaaatacca
     1561 gtgaaacagc aaaacaactt attgaagatg ctaagttaat agaaaaagca ggtgcattct
     1621 ctattgtcct tgaaggagtt cctgcgaaaa tagctgagat ggtaactaat tctatttcaa
     1681 ttcctacaat aggtataggg gcaggaatta actgtgatgg acaaatttta gtttatcaag
     1741 atatgttggg aatgtttgga gattttgttc ctaagtttgt aaagcagtat gcaaatatag
     1801 gagatataat gaaagattca attaaaaatt acatcctaga agttaagaca ggtgcatttc
     1861 cacaagaaaa acacagcttt tcaataaatg aatctgagtt agaaaaatta tactaatatt
     1921 aaggacggta caaaatgctt gtaaaagaaa ttaaattact aagaaatata atcaaagatt
     1981 ggagaaaaga tggctattct atagggcttg ttacaacaat gggattttta catgaaggac
     2041 accaaagtct tataaaaaaa gctgtaaaag aaaatgataa agtagtagtg agtgtatttg
     2101 taaatccaac tcaatttgga ccaaatgaag attttaatag ctatccaaga gatatagata
     2161 aagattttaa atattgtatg gatagcggtg caacagtagt ctttaaccca tcaccagaag
     2221 aaatgtattt aaaaggtaat tgtactacca tcaatgtttc tggacttaca gattttcttt
     2281 gtggagctaa aagacccgtt cattttggag gcgtctgcct agttgtttca aagtttctaa
     2341 atatagtaac acctgataaa gcctattttg gagagaagga tgcccaacaa ttggcagtaa
     2401 ttaaaaggat ggtcaaggat ttaaacatag atacagaaat aataggctgt cctataattc
     2461 gtgaaaatga tggattagca aaaagttcaa gaaatacata tctctctgag gaagagcgaa
     2521 aatctgctct aatattaaat aagagcttaa gtttagcaaa agaagaatta gtaaaaggca
     2581 atctaaatcc aaagaatata aaagaattaa taatagctaa aataaattct gaacacttag
     2641 caaaaataga ctatgttgaa attgtagata gtgaaacatt acaacctgtt aagcaaatag
     2701 aacattctat acttgtggca attgctgtgt ttataggaaa aaccagacta attgataatt
     2761 ttacatttga gttaaatata tagtctataa ttttatataa tatatttaaa aaataataga
     2821 atacctaaaa agtaataaat tacctaaaaa ataataaatt gcttagaaaa taatagatta
     2881 caataactaa aattttaaaa ttaaattcct taaaagtatg attaa
//
LOCUS       DAEMAE010000319         2872 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEMAE010000319 DAEMAE010000000
VERSION     DAEMAE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.324"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..146
                     /locus_tag="KPH93_003552"
     tRNA            73..146
                     /locus_tag="KPH93_003552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Gly,seq:tcc)
     gene            155..271
                     /gene="rrf"
                     /locus_tag="KPH93_003553"
     rRNA            155..271
                     /gene="rrf"
                     /locus_tag="KPH93_003553"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            545..2563
                     /locus_tag="KPH93_003554"
     CDS             545..2563
                     /locus_tag="KPH93_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1690319.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQDLENMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDVIIIGDENAVSKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1151 a          355 c          488 g          878 t
ORIGIN      
        1 attaagatat atgtgtagtt tttagagtgc taactctaaa aaattagttt tactaattta
       61 taaaaattta atgcgggtgt agctcaatgg tagagttctg gccttccaag ccagctgtga
      121 gggttcgatc cccttcaccc gctccaaaag attatgtggt tacaatagca gagaggatac
      181 acctgttccc attccgaaca cagaagttaa gctttctagc gctgatggta cttggtggga
      241 aactgcctgg gagagtagga cgtagccacg taatcttttt ttgttgtatt aaatttaata
      301 aacctacaat tattatatgt ttttatttgt cttttataga tttttttggc aataaatgat
      361 atagtgaaga taattaaata tattcaatat atttaatttt tttatttttt gtttttataa
      421 tttttttaaa agtttatttt ttatgtactt atttataatt ttgggtaaaa acactttgat
      481 atattttcaa aagttaaaag attaaaaaac aaagtagaaa tttatgttaa gtaaggagat
      541 taatatgaga agaaatacaa aattattaac aacagggatt ctttcaatgg caatcgtcac
      601 acctacaatg gcatttgcta ctgaatctaa tgctatggaa aataacgctg atttaaatat
      661 aaacttagag aaaaaaagta tagttttagg tagtacctca aaagtcagtg ttaagtttaa
      721 agaaaaacca gatgcagata gcattacatt aaagtataaa tgctatgata tgccattgga
      781 tacaactcta aattacaatc aatcaactga gtcatatgaa ggaactatca attataataa
      841 agacccagaa tatctaaatg tttgggaact acaagggata acaataaaca gcaaaaataa
      901 tcctaaaact ttaaataaac aagacctaga gaatatggga ttaaatttaa aagactataa
      961 tgtaacacag gaatgtataa ttgaagacat aacttctaga aaagatgtaa ataaatattt
     1021 gagaaaaact tctgcaccta ttacagaact tacaggaagt gatagatacg aaacagcagt
     1081 taaaataagt aaagagggct ggaaaaatgg ttcagataag gtagttataa taaatggaga
     1141 tgtaagtata gatggtatta tatcaactcc actggcaact acatataatg cgccaatact
     1201 tttggttgaa aaaaacaatg tgcctaatag tgtaaaatca gaattaaagc gcctaaatcc
     1261 taaagatgta attataattg gagatgagaa tgctgtttct aaaactactg ctaatcaaat
     1321 taaatcgact gtaaatgcta gtcaaacacg tctaaatggt tctaatagat atgagacatc
     1381 tttattgata gcaaaggaaa tagataaaaa tcatgatgtg gaaaaagtgt acataacaaa
     1441 tgctaatggt ggagaagtgg atgcacttac tatagcagca aaagcaggtc aagacaagca
     1501 accaattata ttaactgata aagatagtat tacagacaat acatataaat ggttaaagag
     1561 tgaggattta caaaatgctt attttatagg tggacctcaa atgatatcaa caaatgttat
     1621 aaataaggta aatgatataa ctaaagataa cgtgactaat aatagagtat atggagcaga
     1681 tagacacgaa acaaatgcaa acgtaataaa aaaattctat acagatgatg aattagaagc
     1741 tgttttagta gccaaatcag atgtacttgt tgatgcctta gcagcaggtc cattggctgc
     1801 caacttaaaa tctccaatac ttataacacc aaagacgtat gtatctgcat accataaaga
     1861 aaacttagaa gctaaatcgg ctaataaggt atataaaata ggaggaggat tgacttctaa
     1921 ggtaatgaac tctatagcat catcattatc taaacacaat acaactccaa cagaaccagg
     1981 aaatagtggg ggtaagacag ttatgattga cccaggacat ggtggttcgg acactggaac
     2041 aacaggtaaa ccattaggag gtattaaaga aaaagactat accttaaata cttcacttgc
     2101 aacaactgaa tatttacgtt caaagggatt caatgtaata atgacaagag atacagataa
     2161 gactttatct cttggaaata gaactgcttt atctaactct ttgagaccag atttatttac
     2221 gagtatacat tataatgcat ctgatacaac tgggaatggt gtagaagtat tttataagct
     2281 taaagataaa gatggaggaa ctactaagac tgtagctaca aatatattaa atagaatttt
     2341 agagaaattt aatctaaaaa atagaggtgc aaaaacaaga acactatcaa ctgaccctac
     2401 aaaagattat ttatatgttt taagaaataa tgatatgcca gctgtacttg tagaatgtgc
     2461 atttttggat aatgagaaag atatgagttt acttaataca tcaaataaag taaaagaaat
     2521 gggtacacaa ataggtaaag gcatagaaga ttcattaaaa tagtctacaa tgagagcata
     2581 gaaaaattta aaataaatta atctgcaatt ttaaatttaa actaaagcta taagataaga
     2641 atgtaattat aatagtaata aagactgtat atacattgtg catataagtg caaaatgagg
     2701 tatatacagt ctttttatga ttaattgtaa aagaatttat attatatgaa gaatttacat
     2761 atcttaagga tacagctaat tgtcaagagt tggagtattc atttgtaata cacaataaaa
     2821 gtttaattac tatattttat tgtgtgtttt tgtgtttttt gtggattttt at
//
LOCUS       DAEMAE010000320         2871 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEMAE010000320 DAEMAE010000000
VERSION     DAEMAE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.325"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..880
                     /locus_tag="KPH93_003555"
     CDS             2..880
                     /locus_tag="KPH93_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1690320.1"
                     /translation="MLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTARQVLR
                     LLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESAFLYG
                     GYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDNVLFV
                     ADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLININK
                     KKMEEIIDNIKKVCYQPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYLYENN
                     MVEYIFDGGKMMIKAV"
     gene            1098..2852
                     /locus_tag="KPH93_003556"
     CDS             1098..2852
                     /locus_tag="KPH93_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG1690321.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKNLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVSKNKGAIILTDGKSVPFKTEGLNIYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMVKYNNMDITPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1196 a          270 c          506 g          899 t
ORIGIN      
        1 aattctagaa catataaaag gaaatacata ttatattcca ctccctacaa tagtaggtgt
       61 atacgtagat ggtaaagatg caattttaat tgatagtgga aacaataagg atacagctag
      121 acaagtttta agattattag aggaacataa tttaagtcct aaattaataa taaacactca
      181 ttcaaatgca gaccatatag gaggaaatgc atatttgaag aatcaaacaa aatgcaagat
      241 tgccacgact aaaattgaag gatattttac agaaaatcca atattagaat cagcattttt
      301 atatggtggc tatccaagta aagcactgaa gaataaattt ttattagcaa aggaaagtga
      361 agttgattat attatacctt caaatggcaa aattgtagat acagaacttg aagcgatttc
      421 tcttccaggt cattattttg aaatgattgg tgttagaact ccagataatg ttttgtttgt
      481 tgcagatagt cttacaccag agaatataat tactaaatat cattttttct tcctactgga
      541 tatagattca cagtttaaga cactagaaaa attgagaatg ttagaggcag atttctttgt
      601 tccaagtcac tctgttaaaa ctacagatat aaaaagtttg attaatatta ataagaagaa
      661 gatggaagaa ataatagata atattaaaaa ggtttgttat cagccagtta tgattgatga
      721 agttattgaa aaaatgtgtg atttgtacaa tgtcaagctt gatgctaatc aatatgtgtt
      781 ggttggaagt acaatacgtt cttatataac ttatttatat gagaataata tggttgaata
      841 tatatttgat ggtggtaaga tgatgataaa agctgtgtaa atagtaataa ttttcaaatt
      901 atatgatgaa attgttttag tatgactgtt atttgaaaag taatataaaa aaatttatat
      961 aaataagcta agatgattga tataaagtta ctgataattt taaggtaata tatacgttac
     1021 tataatatta tgttattatt tgtaaataag gggataattt ataagcaaat taaagacctt
     1081 aatttacagg aggaatagtg tatattatga ataaaaaatt caaaaaattc agtaaaaaac
     1141 taagtatatt aattttagtt ttgactatgt ttctttttaa taatttaact gcaaatgcac
     1201 agattaaaga agattcaata ataggaattg atagatatga aacagcagga cttatagcag
     1261 ataggcaaac ttatgatact gtaatattag ttaatggaga taaaaatcta tctgatgggc
     1321 tttcatcaag tggattagct ggagctatta acgcaccaat acttttgact aaaaaaagtg
     1381 aaataccaaa agctacttta aatagactag ataataagaa tttaaatgtt gtaaaaaaag
     1441 tatatataat tggtggttat aatactattg aaaattcagt agaaaaagat ataaaaagaa
     1501 aaggtattca agtggaaaga ataaatggga acaatagaat tgaaactagt tataatgtag
     1561 ccaaaaaaat aaatgaagta agtagtgtaa aagaggtaat gcttacaaat ggatttgtag
     1621 gagaggctga tgctatgagt atagcacctg tagtttcaaa aaataaagga gctataatac
     1681 ttacagatgg gaaatcagtt ccatttaaga ctgaaggttt aaatatttac gctataggcg
     1741 gcaaatctgc gataaatgag gatttagtaa atgaaactaa agcaactaga atcggaggaa
     1801 atgatagatt tgagaccaat aaaaaggtaa tggaaaagtt ttataatgga gcgacagatt
     1861 tttatataac aaatggatat caattagtag atgcactaac attatctcct ttagccaaag
     1921 aaaaaccaat cgttttagtt gctgatggaa gtaataaagg tattttaaaa ggagctaaaa
     1981 gtataacaaa agttggaggt attgatgcca atatatataa acagtgttta gatatggtta
     2041 aatataataa tatggatata actccaaata ttttaaaaca cttaacttca gttgaaggaa
     2101 atgaagtttc tgaaatactt atagaatcag ataatttagg tgtggttgtt gaaaagacga
     2161 ataccaataa atttgagttt gattacgtaa gtgtaactaa tgaaaaaaat tgtacttttt
     2221 ctgtatataa agaaaattct tctaataatg taaaaaatgg aaagctagtt gtaagtgcta
     2281 agaaaaaaat agataagaaa gatagtgatt tgcaagatat gaatggcgac aatatgataa
     2341 atgcaaataa agataggatg gtaaatgtga taaaaattgg agttcccgat aaaatgtatt
     2401 ctaattttaa agtagatgtt gaaagtggtt cagtagaact ttataatatt aaaggaggag
     2461 ctactgttga tgtcaatgac ggaattgcta acattgttga taacaatgta acaaatcctt
     2521 tcaatataaa tactaatagt gggatttcag gtgtaacagc tgaaactata tcatctgaga
     2581 taaaatttag agcaaacaat ggaattgtaa atgtaacagc tacaaatata aatggcaata
     2641 taagtttatt tggaagcgat aataaagata cctttgatgg aatatttaaa ttaaatctga
     2701 aaaaagaacc atcaaattta cacttgaagt taattggaaa tgggataaat aaattgccag
     2761 aaggttggtc taaagattat attttaggaa atggacaacc aattattgaa gttaaaaata
     2821 atggcataaa taatatatca attggagaat aaaaacatat gaaaataaag t
//
LOCUS       DAEMAE010000321         2856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEMAE010000321 DAEMAE010000000
VERSION     DAEMAE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.326"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            205..492
                     /locus_tag="KPH93_003557"
     CDS             205..492
                     /locus_tag="KPH93_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690322.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKSDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(1031..1582)
                     /locus_tag="KPH93_003558"
     CDS             complement(1031..1582)
                     /locus_tag="KPH93_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690323.1"
                     /translation="MSINTIIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SMHTYHNQGEKTLKAVIINYYPV"
     gene            1773..2735
                     /locus_tag="KPH93_003559"
     CDS             1773..2735
                     /locus_tag="KPH93_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG1690324.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVEQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLDA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT         1113 a          293 c          454 g          996 t
ORIGIN      
        1 gatataaaaa ataattacct tataagtaga ttgaaattta tgaatattca tactataatt
       61 taaatataag gagatgacct ttgaaaataa aaattaaaaa atatttaatg ctactgcaat
      121 aggaactaga actacactta ataaatatac agaaattaga tttggctcaa tataaaatac
      181 aaatatagaa tttaggtgtt ttttatgaaa aaaattttat atgctttata tagtttcatt
      241 gttattatag ctaattttag attaaaagaa aaaaattata actttatttt attagcaatc
      301 ttttttatac tgctattaat tcaaaatgga gttttaaaaa caggttatca aaatagagct
      361 acattttatc aagataatta ttttaatgga gaagataaaa gtgataagat aaaatttatg
      421 actataaaga tttttatatt tttgtcatta ccattgtgtt ttaatatact attcaactat
      481 ttaacttctt aaattatatt tagatatagg agtgttatga taaaagtaag cttatgtgtt
      541 atttatatta aatataaagt gcatatagtg ataaaacaaa agtttcatat aaatgaagaa
      601 aaaaatataa aatgatttat ttaactactt aaaaataaac taaaagggat aataaaatag
      661 cttttaggtc atggtaataa aggtatttaa aaagagtgca taagcactcc aattgtgaga
      721 aatacataca caaatcatta ttacaacaat gaaaaatgta catataagag acgttttgta
      781 tttgttttaa gtgataatat tttaatacta actaaataat tatcacaata gtaaaaaaga
      841 aaaaggagta ttttgtactc catttgtcaa aaatataaaa cttttatata agcttattat
      901 aacataattg ataaaagtgt aaagatatat aatgaattta taaagaaaaa taaaataaaa
      961 aagataaatc ctaattaaag gatttatctt tttgtataga aaataattat tatattatta
     1021 aatggcatgg ctatactgga taataattta ttattactgc tttcaatgtt ttttctcctt
     1081 gattatggta tgtatgcatt gattcggctg aaaaacaaat agagtcattt tctctaagtt
     1141 gataaatgga gtcatttact tctaatttta attgaccttc tataatcata atatattctt
     1201 gtgatttttc agaatgacca acagatgtat aactatgacc ttcttctaat tccatttgaa
     1261 ataattcgaa gtttctagtt ggtgtattgg gataataaca ataaagacga taatctttat
     1321 tctctgaaac ttgtacatct atatcagttt ttgatacaat aaaagtttca ttttgaggtt
     1381 gttctagtat tgcagtatat ggaacattca gtccacttgc aattttccaa attgtgttta
     1441 ctgtggggtt tgagtctccc ttttcaatct gtgaaagcat tactttactt acaccagaca
     1501 attcagcaag ttgacctaga cttaagttac gttcattacg taatctattt agatttttag
     1561 caataatagt attaatactc ataatagctc cttaaaatgt aaattattga caaatataat
     1621 ttacgaatgt ataatttatt taacgttatt tataacttac aaatgtataa tataatgaac
     1681 gttaaataca ctgtacatcc gtaatataaa ttatactgca taaagagttg tttttcaacg
     1741 gatgatagtt gagagattaa gggggaattg taatgcgtgt acttaataat gttacagagg
     1801 ctataggcaa tactcatatg ttacatttat ccaaaatagc agatgaattt ggagttcaag
     1861 gtaatattta tgcaaagatg gaatacttaa atccgggatt tagtaaaaag gaccgtgtag
     1921 cattacaaat gattactgaa gcagaaaatg caggcttatt agttccaggt caagcagttg
     1981 tggaattaac aagtggtaat actggaacag gactagcaat agcttgtgca tgtaaaggtt
     2041 ataaatttat agcgtgtatg tcaaaaggaa attctatgga aagagcaaga atgatgaaag
     2101 ctttaggtgc tgaagtagtt cttgtagagc aaatgcctaa ttcagttcat ggacaggtat
     2161 ctggtgaaga tttggcactt gtagaaattg aggctcaaaa aatagcaaaa gaaagaaatg
     2221 catttagagc agaccaattt aatttagacg cttgtaatca tgcacatgaa aagtatacag
     2281 cagaagaaat gtgggaacaa ttggatggaa atattgatgt atttgtagat tttcttggaa
     2341 gtggtagtac ttttgcagga tgttcaaaaa cacttaaaaa acataattct tctatcaaat
     2401 gttatgttgt tgaacctgaa acagcagcaa tttattcagg taaagaaata actgaccaag
     2461 ggcataaaat acaaggtgga ggatattcta tggatttgcc attagtagac aagaaactta
     2521 ttgatggtta tattcaagtt acagacgagg aggcaactga tgtagcaaga aaattagcta
     2581 aattagaagg tgtatttgca ggattttctt caggggcaaa tgtgtgtgca gcaattaagt
     2641 tgttaaagag cagtgaaaaa ggaaaaaata tagttttaac tcttaatgat agtggtttaa
     2701 aatatttaag tactgattta tatgaagaat tataataata gcacaatagt tatgcaatgt
     2761 ttttaaatta agtatataga tttaaattat aaaaagtatc tcaatgtaat ttttgaaagt
     2821 tataaaggag atactttttt attttgtaaa tatgga
//
LOCUS       DAEMAE010000322         2822 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEMAE010000322 DAEMAE010000000
VERSION     DAEMAE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.327"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            181..2316
                     /gene="rnr"
                     /locus_tag="KPH93_003560"
     CDS             181..2316
                     /gene="rnr"
                     /locus_tag="KPH93_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG1690325.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDSIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLS
                     SGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1232 a          259 c          527 g          804 t
ORIGIN      
        1 ttgtttgaag ttagtatgac aaaacttaca ttaaatcaaa aatataaata agtaatttta
       61 acttgtaata ataagaagaa aatatagaaa attatgtgat aagtaaaaaa tgttttgtct
      121 gaagaaatat aaaagacaat ataaataata atataatgaa aagtaaaaca ggaggtaaaa
      181 atgataccag gacttaaaga aagtttgcta ggattaataa atgaaagtgc atataatcct
      241 ctaaaaaaag aagaattagc agaaatattt gatatacatt catcagaaat gccaatgttt
      301 tataattttt taaatgaatt agaagaagat ggttatatct gctttactaa aaaagggaaa
      361 atagtatctc ccaatcaaat gggatacttt gttggaaaat ttgtatcaca caaaaagggt
      421 tttggatttg tagaatctga tgttgaatat acacaggatt tatttatctc aagtgacagt
      481 ataaatggtg ctatgcataa tgatagagtt atggcagaaa tagtagtacc tgcaactgaa
      541 gaaaagagag ctgaaggaag aatagtaaaa gtaataaaga gagaaataac tagaatagta
      601 ggtactttcc aatcaagtaa aacttttgga tttgtaacac cagacaacaa gaaatttact
      661 aaagatatat atatacctaa aaaacatttt agtggagcag tagataatga taaggtagta
      721 tgcgaaatta cagtatggcc acaagaggat agaaaaccag aaggcaaaat tatagagata
      781 ctaggtcaaa aaggtgaaag aggcgtagaa atagattcta taataagaga acatggtctt
      841 ccagaagaat ttccaaagaa agtattgcaa gaagcagaag ctgtagctgt tgagattcca
      901 caagaagaga taaaaagaag aagagattta agagatttaa acatatttac tatagatgga
      961 gatgacgcaa aagacttaga tgatgccata tcaatagaag tattatctag tggaaacttc
     1021 aaactaggag ttcacatagc tgatgttaca cattatgtaa gagagaaaaa taaacttgac
     1081 aaagaagctt taaaaagagc tacatcagtt tatttagtag ataaagtaat tccaatgcta
     1141 ccaaagactc tttcaaatgg agtatgtagc ttaaatccat ttgaagataa gttaactctt
     1201 tctatattta tggaaataga ccataaagga aatgtggtta aacatgaaat atgtgaatct
     1261 gtaataaatt caaaagctag aatgacttat acagaagtat ctgatatatt agaaaaggat
     1321 gatgaaaaac ttaaaaagac ttttgaacat gttgttgaag attttaaaaa tgctgaaatt
     1381 ttagctagga ttcttatgtc aagaagggaa aaaagaggag ctatagactt caatttccca
     1441 gaggcaaaga taattctaaa tggaaaaggc gaagttgtcg atataaagcc atatgaaaga
     1501 agaatatcta ataagataat agaagaattt atgcttataa gtaatgagac aattgctgaa
     1561 catttttact ggatgggaat accgtttgtg tatagaatac atgagacacc atctttggaa
     1621 aaaatggaag aattgagtaa gtttatatct acatttggat atataataaa aggtgataaa
     1681 gaggaagtac atccaaaagc attacaaggt ataatagaga agataaaggg caaaaaagaa
     1741 gaaggagcta tcagtactat aatgcttcgt tctttaaaac aagctaagta ctcaccagaa
     1801 tgtagtggac actttggatt agcagctaaa ttctactgtc actttacttc gccaataaga
     1861 agatatcctg accttcaaat tcatagaata ataaaagaat ctttaaataa taaaataagt
     1921 ggaaaaagac aagaacagct tactactata gtggattatg catctgttca atcatccgag
     1981 aaagaaagaa aggcagagtt agctgaaaga gatgtacatg atttctataa ggctctttat
     2041 atgaaggata aagttggtca agaatttgag ggtgttgtat ctagcgtaac ttcatttgga
     2101 atatttgttg agttagataa tactatagaa ggattgataa gacttgcaaa tatgaatgat
     2161 gattactata tttatgatga gagttcttat agtgttttag gagaaagaac taagaagtca
     2221 tataagatag gagaccttgt taagataaag gttgaaagtg ttaatgtaga ttttaaagaa
     2281 attgactttg agatagttga aaaaatagag gagtagataa aacagctaaa ataagagata
     2341 acaagttgta gtatttatga tatgtataaa tatatagtgc acaatactct ataaaataaa
     2401 tgataaaaat aggtaattga gaaatatcta atggaaatag ttaactgaaa gataaataat
     2461 taagaaatat ttattgaaag tagataattg aaaaataagt aattgagaaa tgtttaatag
     2521 aagtggctaa ttgaaaataa gtaattaaga aatgcttaat agaagtggct aattgaaaat
     2581 aagtaattaa gaaatgttta atagaagtgg ctaattgaaa aataagtaat taagaaatgt
     2641 ttaatagaag tggctaattg aaaaataagt aattaagaaa tgcttaatag aagtggttaa
     2701 ttgaaaaata agtaattgag aaacatttaa tagaagaagc tagctgaaag ataagtaatt
     2761 aagagatatt taatgtaaac agataattta aaaataccta attaagaata gatattaaaa
     2821 aa
//
LOCUS       DAEMAE010000323         2805 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEMAE010000323 DAEMAE010000000
VERSION     DAEMAE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.328"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..721
                     /locus_tag="KPH93_003561"
     CDS             23..721
                     /locus_tag="KPH93_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690326.1"
                     /translation="MKISSQYRSQYSFRHESNVNNTRINESIVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKDRQEIYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNRIIED
                     KNKSTMLDENSTYLNTNDEKESLIAKEFNEKVEEEILNDEKLEELEQKKDYKEDSNKK
                     ETVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLV"
     gene            765..1313
                     /locus_tag="KPH93_003562"
     CDS             765..1313
                     /locus_tag="KPH93_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690327.1"
                     /translation="MEGLGFYVAALASGSYQRIGFGKLNPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLK
                     DATREKTTYYDAKALSILGFNQ"
     gene            complement(1708..2664)
                     /locus_tag="KPH93_003563"
     CDS             complement(1708..2664)
                     /locus_tag="KPH93_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1690328.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRITDDVIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSKILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         1092 a          314 c          408 g          991 t
ORIGIN      
        1 ttattttaga gaggtaaaat aaatgaaaat atctagtcaa tatagaagtc aatattcatt
       61 tagacatgaa agtaatgtaa ataatacaag aataaatgaa agtatagtta agaaaaatga
      121 aactgtagga aaagacactt atttatctaa tattatgaaa caaaagcaag aacttaatga
      181 tagaattaga gatttaaaag atagacaaga gatttatact aaaaaaataa atgaagcaat
      241 taagaactta tataaatcag aaataagaga aacaactaat aacttttcta atatagaaat
      301 aggtattaaa aatagaatta tagaagataa aaataaaagt acaatgttag atgaaaattc
      361 aacttatcta aatacaaatg atgaaaaaga atctttaatc gctaaagaat ttaatgaaaa
      421 agttgaagaa gaaatattaa atgatgaaaa attagaagag ttagaacaaa aaaaggatta
      481 taaagaggat tctaataaaa aagagacggt atctgaggac ttatccttag taggtaaaac
      541 tcgtgaagag cttgaaaata tgcttaaaaa ttttataaat ttaacacaag aagaaataat
      601 gaaacttgag tcgagaatag aaaagttaga taaagatgct gaagaataca aacaaaattc
      661 aaagactaat atatttgata aaacagatga acaaaaaaaa catataaatg tactggttta
      721 aacaatatgt tttaaaaaaa taagtttatt atataaataa gggtatagaa ggtctaggat
      781 tttatgtggc agcattagcg agtggctcat atcagagaat aggctttgga aaacttaacc
      841 ctatagaagt aatagccgat ggagattgga tttcttacaa acaagctcaa gatacactta
      901 cagtaatcag gaatttttta aatagttttg actggagaaa tgcatctgag atggaaagag
      961 ctaatagagc tgcaaaattg gtaaccgaag caaaatatgc agactctaaa tattgtaaca
     1021 tagtctatgg taatttggtt gataaacgtg gtgtatgtgg aagttttgca agtagctttc
     1081 atttattaac aaggttaatg ggaatggatt cactatctat attaaatccg tcattaaatc
     1141 atgcttggaa ctatatacaa attgatggga aatggtatag gtttgatggg tctgaaatat
     1201 cagcttttgg tggatcatta gactttgatt accgtaaatt aaaggatgca acgagagaga
     1261 agactactta ctatgatgct aaggcattgt ctatattagg atttaatcaa taaaaatagg
     1321 gaaggcttct tatggagact ttttttattg atttgaaatt taataaggaa ttttatatga
     1381 aaatttaatc aataaaagca aaaaaggctc tttataaagc ctttttaagt gtttttttaa
     1441 gtgaaatttt ttatataaca ttagttttaa tcaaaaaata taacattttg gacttacttt
     1501 taaaaataaa attattaaaa tataatatta atcatgttaa taatgttttt agtttattat
     1561 ataatagtgg ttatgttgac tattgtagtt ttattaagaa tagttggttt atataaatat
     1621 atgcaaagat aacttgttgt ttactttatt tattagtata tagtaaaaga tactctaata
     1681 aatttgctta ttttaatatc ttttgcacta aaaccaacta aagtttcctt tattatttcg
     1741 ttttgaataa tacaaagcat aatccgattg tttgtataaa gactggaaag tatcagagtt
     1801 attttctaat ttagatatac ctatagaaca agaattactc tctaggcata attctaaaac
     1861 tttctttgct ttattttcaa cattctcttt tgattgatta ctatattcaa aaacagcaaa
     1921 ttcatctcca ccaagtcttc caataatagc atcatttacg aagactttag tcaaaatctt
     1981 tgaaaaatgt attagtattt catctcctcg aacatgacca aagatatcat ttacctgttt
     2041 aaaattatct aaatcaaaga tgtaaaagta tccatgtgaa acagttttaa gcatttcttt
     2101 tatgtgtact tcaaaaaaat gtctattgta tagtccagtc ataaggtctt tttctgattt
     2161 tagtttaaat tcatttgcca ttgctagtgc ttcttgtgct ttttcagtta ttgttttagg
     2221 gtaatactca tctttcattt ggtcaaagtt tggaatagaa tggtgaacat ggataagttt
     2281 aatcacatca tctgtaattc tgtataccat agtaaatcga gtatccattt ctacatagat
     2341 atctttttct aggtaatctt tttctattac ccaaagagta ccatatacta taaaagcttc
     2401 gtctgtaact ttggtacatg aataccactc atctataatc tcaaagtcta tttgctctga
     2461 ttctgtttgg tctttttcaa gacctataat aaattcttct aatgtttcgt aaaattcatg
     2521 ttttcctgtt ccaattaatg caaggttgtc atctaataag gaaataataa atttaaggtt
     2581 ttcttcttct ggatttaaaa tataaatatg ccaaattttt tttgataaat cccttaattg
     2641 tttttgtatt gtattatttt ccatagatta cccccttaag atttacagtt tatattttaa
     2701 tgtattataa gttgaactct tattacaata ttattataat atagaactaa agaaattaca
     2761 tcctgtatag gtaaaagtat atcttttaca ataaaaatat tacag
//
LOCUS       DAEMAE010000324         2772 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEMAE010000324 DAEMAE010000000
VERSION     DAEMAE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.329"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(195..386)
                     /locus_tag="KPH93_003564"
     CDS             complement(195..386)
                     /locus_tag="KPH93_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690329.1"
                     /translation="MDDFSKLKNLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            605..1651
                     /gene="cobT"
                     /locus_tag="KPH93_003565"
     CDS             605..1651
                     /gene="cobT"
                     /locus_tag="KPH93_003565"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG1690330.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFETSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1683..2243
                     /gene="cobU"
                     /locus_tag="KPH93_003566"
     CDS             1683..2243
                     /gene="cobU"
                     /locus_tag="KPH93_003566"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG1690331.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCMDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPINWNTYEIYEDIYSIIKEIYTGHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIKEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIPMKIKE"
     gene            2253..>2772
                     /gene="cobS"
                     /locus_tag="KPH93_003567"
     CDS             2253..>2772
                     /gene="cobS"
                     /locus_tag="KPH93_003567"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG1690332.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFIYNIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTS"
BASE COUNT         1119 a          286 c          505 g          862 t
ORIGIN      
        1 gataagttaa taatgttaaa tgggaagtga gtttatggaa aaaccaaata caaaagttgt
       61 taactttcaa gagtataaag acagaaaaaa ccaaagagaa gtaaatagag acgaactttt
      121 aaagcttata aaaaaaattg tgacttcaaa atagggagtt gttaaactcc ctattttaat
      181 gggtttcata ataattaaga cattctaagt ccatctatat aatctttgaa ttgtgagaaa
      241 tctgattcac tacttaattc tatagtttct gaaccttccg tataaaaatt cttaagatag
      301 atagtataat tattatcaac acaatcatga taaatacatc tatatccatc ttcatacaag
      361 ttttttaact tagaaaaatc atccacaata tcatctcctt aaagttcttt taaatatatt
      421 tttacaaaaa gcatctaatt gtatgcaaat tgacaatgta aaataaagga aattaacata
      481 aaatgtagaa taatttttat atgtatgtaa aaataaaaag aaaaagttga aatttttaga
      541 aaataaaagt attttttatc taaaaagaga atttaaaaaa taaaaaaatg ttgggaggat
      601 aaaaatgagt ttattagaaa gtatatcaaa aaacatatat tctctagata attcttctat
      661 tgaaaaaaca aagcagagat tggacaggct tatacatcca actggaagct taggaaagat
      721 agaagatatt tgcatgcaat tagcaggaat atttggaaat gaaaattttg agacaagtaa
      781 aaaggttata atagcttttg ctggagacca cggtgtatat gaagaaggag tagcacctga
      841 ccctcaaaat ataacaaaac tacaattccc aaatttttcg aaaggtctat gtggagttgg
      901 agtaataagt aaatttgtag gggcagatgt tgtagctgta gatgtaggaa taaattgtga
      961 tgaaaaactg gatggagtat tagattacaa gataagaaaa gggacatcaa atatggcaaa
     1021 agggccagct atgagtaaac aagaagcgat tagatgttta gagataggta ttgaaatagc
     1081 agagcagtgt atagaaaaag attataaggt tattggtatt ggagagatgg gtatagcaaa
     1141 tactactcca agtacagcaa taatatctgt aatttcaggg tgtgacccat tagaggttac
     1201 tggtataggt gcaggtctta agaaagagag actaaagcat aaggctgaag taattagaaa
     1261 ggctattgaa attaataatc caaatccaac tgatggagta gatattttat ctaaagtagg
     1321 aggatttgaa ataggctcaa tggcaggtgt tatattaggt tgtagtgcta atagaatccc
     1381 cgtagtaatt gatggcttta tttcttatgc agcagcatta attgcatata agataaatcc
     1441 aaaaactaga gaatatatga tagcatccca tttgtcagca gaatcaggaa ctaaaagagc
     1501 attagatata ttgaaattag accctttatt aaatatggat atgagattag gtgagggaag
     1561 tggagctgct ttagcatttg atataataga agcttcaaat tatacttaca aaaatatggc
     1621 aacttttgat gaaattgaca tgggaagata aatataaaaa tttaatatgt aggagtgttt
     1681 tgatgagtaa aattattcta gtaacaggag gggctaggtc agggaagagt aactttgcag
     1741 aaaaactatg tatggacaga aataataata cagcttatat agcaacttct attccatttg
     1801 atgatgaaat gaaagataga gtgaaaaagc atcaagagaa tagacctata aactggaata
     1861 cctatgaaat atatgaagat atatattcca taattaaaga gatatataca ggacatgaaa
     1921 ctgttatatt agattgtgta acattattag taaataatct aatgtttgaa cacaatttaa
     1981 atatagaaga gtctagtcaa gaagaaataa acaaattaga acaatacata aaagaacaag
     2041 ttgtcaaact tattaaagaa atagaaaaaa caaatctata ctttgtattt gtgacgaatg
     2101 aattaggtat gggaattgtt cctgggaata aacttagtag gatttatgct gatattgtag
     2161 gtcgcataaa tcaatatata gcatcaaaaa gcaatgaagt ttattttgta gttagtggca
     2221 tacctatgaa aataaaggag tagtatgtga ttatgaaaag atttatttta atactacagt
     2281 ttttgacaag gatacctata aaattaaatg taggatttga tgatgaattt tataagtcaa
     2341 tagtatattt tccacttgta ggttttgtca taggtatatt atcctattta ataggatgga
     2401 tttctatgct tttatttgaa ccatttatag cttctataat aataactcta gctggtgtct
     2461 taattactgg aggacttcat atagatggat taggagatac atttgatgca atatatagtt
     2521 atagggataa agaaaaaatg cttgaaataa tgaaagattc aagattaggt acaaattcac
     2581 ttttagcaat tatgtttgta cttttattaa aagttggatt catctacaat ataataagta
     2641 ataattcatt atgggttata atttttatgc caatgattgc aaggctagga gtaatgctgt
     2701 tgacatataa aactgtgaca ccaagagaaa aaggaatggg aaatttattt ataggaaaat
     2761 taactacaag ta
//
LOCUS       DAEMAE010000325         2751 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEMAE010000325 DAEMAE010000000
VERSION     DAEMAE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.330"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            699..2687
                     /locus_tag="KPH93_003568"
                     /pseudo
     CDS             699..2687
                     /locus_tag="KPH93_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1188 a          344 c          463 g          756 t
ORIGIN      
        1 tagatgattt atgtaagatt tatgaccaat ttgtgtatga tttgtgttct ttaatcatga
       61 caatattata ttgtaaaaaa taaatttaat aaaaaatgac ttggaattta tattctaggt
      121 cttttttata taaaaattta ataagtttca aatgttggta actttgacag tagaaaacgt
      181 ctattgagat aacgaggatt ctaattgagg gggagatata ttagcactta aagtagtata
      241 tgcaacagat ataatcttta taaaagtaat tcgaattaaa atgtaaatat aaagaataat
      301 taaaaaaata tcgcaattat ttgtttttca tatggataaa attggttatt taatatataa
      361 ttacaagtat aaataataaa atttatttgt aaaaagagta gcacttttaa ttctaaaggc
      421 tacttttttt atacaaaacc atgtttttta taacaattca ttaacataaa aatcaaactt
      481 accttaaaat ataaatttac aaatttttca atcaataaga aaatttcttt attccttaca
      541 taatgactgc aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa
      601 cctctttaaa attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa
      661 ttaagtaata tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg
      721 ctgcaatgct tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac
      781 aaactgtggc aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc
      841 aagatggatg gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg
      901 ctctatctgc aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag
      961 ataaattaaa tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact
     1021 taataggtgg aacttctgta ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa
     1081 tatcttttga gagaatctct ggagctgaga gatatcaaac atccttagaa ttggcaaaga
     1141 aattagatgc tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg
     1201 cagatgctgt aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg
     1261 attctaaaaa tggaacagca gttgcagaca aatttataaa agatgctgga ataactcaat
     1321 cttatgtagt aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata
     1381 gtacaagact aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt
     1441 ataaaaagac agatttaaaa aatgcttatg taactaaaga tggaatgaat aaacaagacc
     1501 aacttataga tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt
     1561 tagttggaaa gaacctatca gcttcacaaa aatctttagt taactctaag agttttgata
     1621 agataactaa agttggtgga aatggtaatg agacagcatt taatgaaatg aaatcactac
     1681 aagaagtaaa aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag
     1741 ctactgctaa tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa
     1801 tacaaactga taaagcagta acagtaaact taaatggtac atatacaaag actgtgacta
     1861 tcaacatgcc taatggtgat gtaaataact atgcaaaagt ggatgatgta gttatagatg
     1921 atgtaaaaga tggaactttt gtcaactatg gaaaaataac taacttaaag gttaatgata
     1981 aaaatggagc taagatagag aataattcta aaggtgagat tggtagttta acagtagcat
     2041 ctggagcaag tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt
     2101 ctaaagggac tactatagat aataaaggta caataagtag tgttaaggga gataattctc
     2161 caacaataag tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag
     2221 gttcttcaca tggaggaggc tcatcttcag gaggaagttc ttctaatcaa acatcagtaa
     2281 ataatgaagc agcaaaaata acaagcgtac ctacaccagc aaaagatgca actaaattaa
     2341 ctatgccatc agtatcaagt ggatatagta tagcaataaa aacatcaagt aatgagagcg
     2401 ttataaaaaa agatggaaca ataatacctc caaatacagc aacaacagta aaattagtat
     2461 ttacagtaac acatacatca agtggtaaaa cagcagatac aaaagaacta tctgtaacag
     2521 taccagctaa atcaacagaa gtacaagcag cagtatcaac tgtaaacgca acaaatggaa
     2581 cattaacaat agtattagat aaaacaccaa cagtagaccc agttgaagga gattttacag
     2641 caaagaaatc aatagatgga ggacaagaat cagaattaac attaagtaac tttgcttaca
     2701 acaaagagtc taaaacagta acatattcat ttactccaat agaacaaacg g
//
LOCUS       DAEMAE010000326         2741 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEMAE010000326 DAEMAE010000000
VERSION     DAEMAE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.331"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..2018
                     /locus_tag="KPH93_003569"
     CDS             192..2018
                     /locus_tag="KPH93_003569"
                     /inference="COORDINATES: protein motif:HMM:NF022062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HBG1690333.1"
                     /translation="MSKTTIISHPVIKSYIDNFIAEHEISKSKAKDQHTIFEQYINDL
                     VLSIYSNDPSVTYQDMDTDTAFGIDGIAIFVSDRVVTSIEDVNSIIDEIRKFEVSFFF
                     TQSKTSDKFNRQDISDFFTGVRRFFNFDRCEIPELYTHWEVAKYIYSKGARFKKQPSL
                     NMKFICLSPKEIDLGNIHMKSEIDLGIADLNDMSLFNEVKTPEFLGIKDIMSLHEKNT
                     SGLEVSITLTKQPVPYPKDIHNRIKNGYYGLIKLEEFISLLTDEVNNERILRKGIFND
                     NIRYYLGSSEKVEVNASMKKQLLGDENYLFGLLNNGVTIIADNIFLSSEDLTLTNYQI
                     VNGCQTSNVIFECLNSIDNKTNDIYIPIRLISTEDEDTKHAIIKATNSQTQLKPEQLV
                     ALSSIQKAIEQYYNTKTRSSTCPLYYERRTEQYRDENIAKTKIINIPFQIKATSALFF
                     DLPHEVSGQYGKVVKQTGDLLFKDNHLSFLNSYYVSGLAWYKVERFVLNNDDGKKYRR
                     ARWHIIMLLKYLCCPHDKINLSIDKQSEENSLIIERILLDDEKTNTILKEAIEIIELS
                     LSQKEDIEEILADRKTFERKDTTTIIIEYVKKYITEKNVVNV"
     gene            complement(2433..2603)
                     /locus_tag="KPH93_003570"
                     /pseudo
     CDS             complement(2433..2603)
                     /locus_tag="KPH93_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1085 a          349 c          339 g          968 t
ORIGIN      
        1 tattttaaga ttaatttcct ttaaagattt ataatatatt tagtaataaa aaatatttaa
       61 ttggtaaaaa tattcaatta tgttattaat atttaataat tattaaatac acaaatatat
      121 gttatttaca gtcgatttat gctataattt ataaaagtag aatatagaaa tcaattgaaa
      181 ggatccttat tatgtctaaa acaacaatta tatctcaccc agtaataaaa tcctatatag
      241 acaattttat tgctgaacat gagatatcta aatctaaagc aaaagatcag cacactattt
      301 ttgaacaata tattaatgac ttagttctat ctatttattc taatgaccca agtgtaactt
      361 atcaagatat ggatactgat acagcttttg gtatagatgg tatagctata tttgtctcag
      421 atagagttgt aacaagtatt gaagatgtta attctattat agatgaaatc aggaaatttg
      481 aggttagttt cttttttact caatcaaaaa catctgacaa atttaatcgc caagatattt
      541 ctgatttttt tacaggtgtt agacgttttt ttaactttga tagatgtgaa ataccagaac
      601 tatatacaca ttgggaagta gcaaaatata tttattcaaa aggtgcacga tttaaaaaac
      661 agcctagttt aaacatgaaa ttcatatgtt tatctccaaa agaaatagat ttaggtaata
      721 tacatatgaa atctgaaata gatttgggta ttgctgattt aaatgatatg agcttattca
      781 acgaagtaaa aacacctgag tttttgggca ttaaagatat aatgtctcta catgaaaaaa
      841 acacctctgg attagaggta tcaattacgt taactaagca acctgtacca tatccaaagg
      901 atattcataa tagaatcaaa aatggatatt atggactaat taaattagaa gagtttatta
      961 gtttattgac tgatgaagtt aataatgaaa ggattttgag aaaaggtatt tttaatgaca
     1021 atatacgtta ctatttagga tcttctgaaa aagtagaagt taatgcttcc atgaaaaaac
     1081 agttattggg agacgaaaat tatttatttg gcttattaaa taatggagta acaattattg
     1141 ctgataatat ttttttaagt tctgaagatt taacattgac taattatcaa atagttaatg
     1201 gctgccaaac aagcaatgtt atttttgaat gtttaaattc tatagacaac aagaccaatg
     1261 atatttatat tcctattaga ttaatttcta cagaagatga agatacgaaa catgctatta
     1321 taaaagctac taatagtcag actcaattaa aaccagagca actggtggct ctatcttcta
     1381 ttcaaaaagc tatagaacaa tactataata ctaaaacaag aagttcaact tgccctcttt
     1441 attatgaacg tagaacagaa caatacagag atgaaaatat tgctaaaaca aaaattatta
     1501 atattccatt tcaaataaaa gcaacatctg cattattttt tgatttacct catgaagtct
     1561 ctggacagta tggtaaagtt gtaaaacaga ctggggattt attattcaag gataaccatc
     1621 tatctttctt aaattcttat tatgtaagtg gtcttgcttg gtataaggtt gaaagatttg
     1681 tacttaataa tgatgacgga aaaaaatata gacgtgctag atggcatatt attatgcttt
     1741 taaaatatct ttgctgtcct catgataaaa taaatctttc tattgacaag caatctgaag
     1801 aaaacagttt aataattgaa agaatcttac ttgatgatga aaaaacaaat actatattaa
     1861 aagaagctat agaaattatt gaactaagtc tgagtcaaaa agaagatata gaagaaattc
     1921 ttgctgacag aaaaactttt gaaagaaaag atactacaac tattattatt gagtatgtaa
     1981 aaaaatacat tactgaaaaa aatgttgtta atgtttgatt ttgaagacgt ttctacaaaa
     2041 agaaacgtct tttctgtgtt taatcattat caaaaaacag aatagcaatt atcaatgata
     2101 ttccattaat aaaattctaa acatacttat attaataatt caaaatatgt ccatattctt
     2161 aaaattgagt aaagctaaaa taaaatattt ttaactctga taatttgtca ccaaataaaa
     2221 acactaccat aaataaacat cagcaatttt tatcttatag cttttagttc tcaagaaatt
     2281 ttaattggta ttattttcta tattttttta ctatcaaata ctaaggaaat aatcactttt
     2341 ctttatataa ttttctacca tcacatagca ctaacattaa atcaaaatgc cactcttaat
     2401 tgtctcaaac taaaaagata ctgatagctt tattagtcat caatatcttc ttcgttcaaa
     2461 tttttaaaat attcttctaa tttgttcaag tttttactat ctaaaacttc atcatcatgt
     2521 aatgctgaaa taaaactctt aaacaaattg ttatgcataa aactaaagaa actctttgtc
     2581 tcaactttta aataatcttt ctctagtcag taagtatata tttttatacc ttctaaaaat
     2641 acatccaaat catcaaactt tcttagcaat tactttatca ttatataatg tcaaatcaaa
     2701 gctctcaaat aaattggtat tcatatagca aacaaatact t
//
LOCUS       DAEMAE010000327         2734 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEMAE010000327 DAEMAE010000000
VERSION     DAEMAE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.332"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..835
                     /locus_tag="KPH93_003571"
     CDS             104..835
                     /locus_tag="KPH93_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1690334.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLEDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            837..2195
                     /locus_tag="KPH93_003572"
     CDS             837..2195
                     /locus_tag="KPH93_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1690335.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLINFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFYSQILFFLMTFLFVKYIIKQSTALSTVSTLLV
                     YLIEQLVYGFTAPLNYLILISDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYLAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFNKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQITNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            2234..2719
                     /locus_tag="KPH93_003573"
     CDS             2234..2719
                     /locus_tag="KPH93_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690336.1"
                     /translation="MYLNRESLIYGNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEIEDIENKIQELKGNKAKQTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSSADEQWDNTYRVLQDDKNLKVKFNRP
                     I"
BASE COUNT         1105 a          264 c          405 g          960 t
ORIGIN      
        1 tatactatga aaagtgagat tattacattt tggaacaaat attcaacata ttagatcaga
       61 ctaattgaac agaaaaaatt aactttatat aataattaga ataatgataa gaaagaaggt
      121 gtatatattg atttcaattg ccatttgtga tgatgaatcc tatatgctaa atcatttgaa
      181 ggaaaatata gaattataca tgaagcagaa gaatttatta tataatatag agctttttaa
      241 taaagtagaa gatttactat gcaatcatga aaagtttaat attgtatttt tagatataca
      301 gatggaagag attaacggaa tgcaggcagc tagaaaatta aggtcatatg gtaatgagtg
      361 ctttataatt ttcgttacag ttttaaatga atttgtattt gatgcttttg aagtagatgc
      421 tataaattat ttgataaaac ctctatctaa tgaaaaacta tataaaactt tggataaaat
      481 agttacaaaa cttgaggata atgaaagtaa ttatatcaca atacaaaaag gttattcata
      541 ttggaaatta aaacttgctg atattttata ttgtgaagtt ataaaaagaa agatttatgt
      601 tcatttaaag aaagagactg tagattatta tgagactatt gaaaatttag agagaagttt
      661 accaacaaat ttttttaggt gtcatcgtag ttatataatc aatttacaat atgtatatgg
      721 atatgaaaat ggtaatgcag ttatggaaag tggagataga atacctgttt ctagactacg
      781 tcaacaaata ttttcaaaag ttatgcttga gttcatgaaa aataggagga tataagatga
      841 ttaatttatt ttcttctgat ttatttttaa agtacagttt tttattctct tttattgtat
      901 atagaatttt aatggtaata gtgcaatact attttctaat caattttaca aatgttaatt
      961 caaaattttg gtgttttatt ttatattcga taatttcaat tttcttaatc attataaata
     1021 catattttaa tttatataat ttattttatt cacagattct attttttctt atgacttttt
     1081 tatttgtaaa gtacataata aaacaatcta ctgctttatc tacagtatca actttgttag
     1141 tatatttaat tgaacaatta gtatatggat ttacagctcc attaaattac ttgatactta
     1201 ttagtgacat aaatacagat atttataaat ttgatataaa catgggacta tcttctttaa
     1261 ttactattat aatagctgga tttatatatt ggtatagttc taaaaagttt aatattaaag
     1321 ttatgaactt tgataaatat ctagcaattt tgatgattcc attgttgctt ataatattat
     1381 ttatgcaatc atttgaatat agttcaaata taaatataga tacaagtctt gggattgtaa
     1441 agctgctatt aaatacttct ataactgaag aaattcaagc ttttcttttt tctataattg
     1501 gaactatatg tgtatttttt tctctattta cctttaataa actaattcaa gctctcgaag
     1561 atgacaagga gagggcaatt atgaatcaac agattcatgc tcaaaaaaac tatattgaag
     1621 aagcgaaatc acgattatca caaaccatat cctttagaca tgattttaat aatcatttag
     1681 ctattgtcaa tggattgttg aaaaaagatc aaattttaaa agcacaagat tatttaaata
     1741 agcttgaaaa agtaacaagt gacctatcat tttcttgtaa tacacagaat attgttattg
     1801 atgctctttt caacaataaa ttaagtatag caaaacaatt aggcatagag gtagattgtg
     1861 aagtaattat accccaaatc actaacatga acgattttga cctatgtata gtgtttgcta
     1921 atgctattga caatgcaatt aaagcttgta gagttgtaga taaacaaaat aggtatttaa
     1981 agctttccac taagttagag ggtggatttt ttatgataga aataaaaaat agctataatc
     2041 caataaataa ttatacaagt ggttcaggta taggtctttt aaatataaaa gaaattgcta
     2101 aaaaatatca aggggctata agtatagata agacatcaga ctattttcaa ataaatgtat
     2161 tgctagttat ttcattacat tgcgctagta tttgagaaca atttcattga aaatagtatt
     2221 tgtaagctat atcatgtatt taaatagaga aagtttgata tatggaaatg aggaggttac
     2281 taaaatgaaa gttggaaata taaatggtca agcaccaatt acaaatggtt tagatagttg
     2341 tcaaagtgag gtacaaggct tagaacaaaa gaaaaaagca ttacaggatg aaatagagaa
     2401 agtaagaagt gataaaagta ttgatggtga aacggccaaa ttaaaaatag aagctttaaa
     2461 aaaagaaata gaagatattg aaaataaaat tcaagagtta aaaggaaata aagcaaaaca
     2521 aacaaataaa gaagaaaata acgtttcaga agtaaataat aatctattag agagaaaaaa
     2581 agaaccttct aataatgaaa atgtaaaaca attatatgat gtttttgaaa gaagcagtgc
     2641 agacgaacaa tgggataata cttatcgtgt tctccaagat gataaaaact taaaagtaaa
     2701 atttaataga cctatatagt ttatttataa aaat
//
LOCUS       DAEMAE010000328         2704 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEMAE010000328 DAEMAE010000000
VERSION     DAEMAE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.333"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            363..2174
                     /locus_tag="KPH93_003574"
     CDS             363..2174
                     /locus_tag="KPH93_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1690337.1"
                     /translation="MKKKYDFLIYAAIWLVIIILFIFSLYHSIEHIKIISHTSTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGDGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNEKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATALSKTFNIKETCARIGSDNFVILAKYRDSILEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKALENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTIKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2262..>2704)
                     /locus_tag="KPH93_003575"
     CDS             complement(2262..>2704)
                     /locus_tag="KPH93_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690338.1"
                     /translation="YMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWDEIHCW
                     DEKIATEKTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALG
                     SEDGEYFELTNDSILYLLDIEAVEIREQSKNIGEFLYKYKKRFK"
BASE COUNT         1023 a          279 c          400 g         1002 t
ORIGIN      
        1 atagtatttt aattatttat taaatataga ttatgtatat gctaaattta aaaagaaaca
       61 taaatgaatt atacaaatat attattgggg ggatatttta taagaaaaga agtgatgcag
      121 taaattaatt gagcaattta aaaatgattt aatgataggg ttgatgaatg ttttaaaaaa
      181 aattttagaa aaacgtatat tggtgtttgt gttggaggtg atgaatttgt agaatttata
      241 gagtataaat gattaaacta tttaagataa atacatatca ctgtaaagta cttataatca
      301 ttttttataa gagattgatt atttgcaaat tatatagata tattacaaat gaggtaacaa
      361 taatgaagaa aaaatatgat tttttaattt atgctgctat ttggctagtt attattattc
      421 tttttatatt ttctctatat catagtatag agcatattaa aattataagt catactagta
      481 ctattcgcag tgaaactcaa aaggttgtta agcaagaatt gaataatgaa agaaatgatg
      541 acttgataaa acgactggat aatattctga taaagctaag aacaggtgat ggagaaaatg
      601 ggtttcaaag atgtgataat aaagagtttc aacaaaaatt aaatcaaatg gattctatgt
      661 gggaatctat gaagaaagaa attataaaag ttagaaacgg tgcttcaggt gataaattat
      721 ataaattgag cgaagagtat tctgtgttgt ctaatcaaat tgtttttata tctgaaaaac
      781 actctaatga aaaattgtat tcttttgcca ctgcactatt tatttatctt atattctcaa
      841 caataagtct tttaatttgg gaatattata ataaaaaaag gtttaaaaga atattttata
      901 ctgataattt aacaaaaata aaaaatcaag ttgcatttga aaacagagcg attgaaattt
      961 tacataatgc atctaacaaa gagtatgtac tactaaatat tgatattgac aattttaaat
     1021 acattaacga tacacatgga tatgaatatg gagataaagt tctaataata gttgcaactg
     1081 cactttctaa aacttttaat atcaaagaaa cttgtgcaag aattggttca gataattttg
     1141 taatacttgc aaaatataga gattctattt tagaggatat tcgggaaatg ctaactaatg
     1201 ctataatctc tgaattagat atgaatgtaa ctcaaactat atcttattgt ataggagcat
     1261 atttagttga aattgataat ttaggttata agtctatcaa ttctataatg gataaggcaa
     1321 atattgcaca caaggtaagt aaaacaagag gaatttcctc tacagtatgg tataatgaaa
     1381 atctgttaaa acagttacaa atggaaaata gtatttataa ttatatgtat aaagcgctag
     1441 aaaatgaaga atttcatatg tatttgcaac ctaaatttca aatatcttct ttaaatgttg
     1501 taagcgcaga agctcttgta agatggtttt ctccagaact tggtttttta tcaccagatg
     1561 aatttattcc attgtttgaa aaaagtggat ttattattga attggacttt tatatgttaa
     1621 aaaaagcgtg ttcatttgtc aaaaaaacgt ttatgaaaaa aaatcaatat acatatccga
     1681 ttgcagttaa tttttcaagg gttactattt accaaaatag tttttatcaa aggtttttag
     1741 atactgttaa agaatatgaa attcctttta agtatataga gatagaggta actgaaagtg
     1801 cttttaatga aatatcccaa ccagttattt cgattttaga ggagcttaaa aagttagggt
     1861 ttttaatctc gatggatgat tttgggtcag gatactcttc attaagtctt ttatgttctc
     1921 tatctattaa tggattgaag ttagataaaa gcttacttaa agaaacattt aaccgggaga
     1981 aagtatatag tattattcag tgtattattg aaatgtcaca tcgtataggc atgtctgttg
     2041 tatgtgaagg tattgaaact aaaaaagact tggaattttt aaatacaatt aaatgtgatg
     2101 ttggacaagg gttttatttc tctaaaccga tagaagaaaa agaatttttt aacaaatatg
     2161 taactaaaaa ataatattat tatttttatt agtcaataca ttgagtcatt ttatatctca
     2221 ttaaatgact caatgtattg ttatgtattt gaaaatataa attatttaaa acgttttttg
     2281 tacttatata gaaattctcc tatatttttt gattgttctc ttatttcaac tgcttctata
     2341 tctagtaaat aaagtataga atcatttgta agttcaaaat attcaccatc ttctgaaccg
     2401 agtgcatgtc tctcaatttt taatcctttt tcttcataat atttttttgt ttctatatat
     2461 aatgattctc gcttttgtat gtagtctttc acttcattat ttggaaagtt atatgagtcc
     2521 accaatgttt cgccatttat aaaagttttc tcagtagcaa ttttttcatc ccaacaatgg
     2581 atttcatccc aagtaaaaaa tatctcagtt ccatctggaa tgaatgaaaa cctggtaata
     2641 atatataaat aattattgaa atttacttca taatattcag gaatatcttc aaattccata
     2701 tagt
//
LOCUS       DAEMAE010000329         2664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEMAE010000329 DAEMAE010000000
VERSION     DAEMAE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.334"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1015..1359)
                     /locus_tag="KPH93_003576"
     CDS             complement(1015..1359)
                     /locus_tag="KPH93_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690339.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1486..1869
                     /locus_tag="KPH93_003577"
     CDS             1486..1869
                     /locus_tag="KPH93_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690340.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT         1022 a          323 c          304 g         1015 t
ORIGIN      
        1 tgtatatagg tcagttaatg aataaaaagc cttatcgact ttagataaaa tagattaaaa
       61 ataatgtaaa cagtatactt cagaaattaa aacgtttgga aataactgga atgttgtagc
      121 atcagccttt agttttacta aaataagtaa tatctataat atatattaca agtataatcg
      181 aaggatttaa taaatatttc aaaaatgtct caaaaacaaa gtccagattt acatcaaacg
      241 cttcactctt aaagatatta ttcttgacaa gcgaaaatgt gatgaaaaaa tggactatga
      301 aatataaaaa ttgatataat atatacaatc atttattcat atcttttgat agtagaatat
      361 aattttaaaa attataccct aaaaatttgc tataattgtt ataatatata taatttccaa
      421 aaatatttaa aaacatctca atatacattt tttataattt gttaaaataa aattcatata
      481 ttaagaaata ttaatagtaa ataagcaatt gttactataa cgtaggaatt atctttaaaa
      541 acacaaatta atttacattt tctaatttta aagtatatgt atatctttga ttttaataaa
      601 attcacttaa aatattttaa ggttgaacaa tgaaattgaa tgcccaagaa cttccttctc
      661 ttactaatgt tgcaattaat tcaaacccac aattgtattg aactgaatct ctacctactg
      721 gtttccaact agaaggtgca ttatatgaga aagaatttga tgttggctct ttcttttgtt
      781 gctgcttaat tcctagccca ttagaaataa atccagtttg atttatatgc ccccttctat
      841 ttgttacacg aatctggccg tctgcaatag accagtttcc agaaacttta ctaacggaaa
      901 tatattgaaa tgctccatct ccttttgata ttgtatatgt aactgtacca gatgctttta
      961 caccaccagt tttatcccat ccatcttctg atatacttgc ttttctaaga ggctttattg
     1021 tattagtatc aaaaaaactg tcttagaata tatctttgat gttaaatttt tagattgttt
     1081 aacttctaat aattgagttg ttgatataga atcaacgtat atggactctc ctgttcttgc
     1141 atttttgatt ataccttttt cattggaact tttaacatca gtaattccat ttttagccat
     1201 atcgtataat ttgtccatgt ctgttacttg tttctctgaa tacattactt tcatagatga
     1261 atcttctacc ttactagcac ttgcatttgt tggtaatagt attgtcatta tgagtaaaga
     1321 taaaatagtt gttacagatt tttttaacct ataattcata ttaatcctta ctttttattt
     1381 gttataacag acattctatt gtaatagaat ataaatccac aatattttta aaattagtat
     1441 tgaataacta ttctcatgtt ataattattc aaaggaggga ttgatatgag gaaaaaattc
     1501 tttataggta ttcttatagt tttaataatc tctgtgatag ctatacttaa tataaaaaat
     1561 gataaaaatg tctttattgg agaatcagaa aattggaaag ctaaatttgt aactactgaa
     1621 ggacactcaa aattagaaat aaaatataaa aatagtaaag atgtagttga acaaaaattt
     1681 aattattcct atcaatttat agatggtgaa gatgcagttg tagttgtaaa aaatgaaatt
     1741 ttagaagcag gtcaagaaaa agcaataaaa atacaaagac caggtcttta ttcaaataga
     1801 gaatctagtt acaagctaat tatatcttgg aatggaaatg aggaaactat aaacttgaaa
     1861 aagaaataac catattatca atttttaaaa aattattatt caaattctta taaaaagagt
     1921 gtttagaaaa atgaaacact cttttttgta taagtttttg aaaactataa atgaaatata
     1981 tcaaaatcgt tcgaaaaatt actgaaataa actcttaaat atattataaa ttaaggtata
     2041 ttatacatga atttattggg agtttatgag ttttaaaatt ggaaaccaaa caataagggc
     2101 gaaaaatact ttaaggaaaa tatataaagt tctataaatt atcaaaaata tttcgcaaaa
     2161 attgataatt attacaaata aattgataat attaataata ttagaaatat cgaaattgaa
     2221 caaactagga ttaaagctca attagaaaaa tccactaatc ctgagaatgc tttgttattc
     2281 tcatttatta ctgttgtttt ttcattattt gctaatttat ttttcagttc attaaatgtt
     2341 aaatataact ctataaattt aattttacag ttaatatgta ttatagttct tatatttata
     2401 tatattaata catttttata tcgcctcttc aatttgagcc ccatcattat ataaaattaa
     2461 cctactggat atcttaattt aatatatgta cactattcat atcttaatag tcaatattat
     2521 tcatttttaa tagctctttt tagatgtcag aattattata ttttgaaatt tattaaattt
     2581 ttgcattaaa aaaactagaa attaatttaa gtcttgaatt acttataact ttctacgtat
     2641 ctacctttat attcttactc tgat
//
LOCUS       DAEMAE010000330         2642 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEMAE010000330 DAEMAE010000000
VERSION     DAEMAE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.336"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..454
                     /locus_tag="KPH93_003578"
     CDS             134..454
                     /locus_tag="KPH93_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG1690341.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            592..1236
                     /gene="trmB"
                     /locus_tag="KPH93_003579"
     CDS             592..1236
                     /gene="trmB"
                     /locus_tag="KPH93_003579"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG1690342.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1365..2111
                     /locus_tag="KPH93_003580"
     CDS             1365..2111
                     /locus_tag="KPH93_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690343.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDGVVNSLSNNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGENIDLRKIIMKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2240..2518)
                     /locus_tag="KPH93_003581"
     CDS             complement(2240..2518)
                     /locus_tag="KPH93_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690344.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
BASE COUNT         1044 a          271 c          434 g          893 t
ORIGIN      
        1 taaaatattt taaagcctta ggtaatatta ggtagaagtt ttagtaaaat atgcaaaaac
       61 taattgatta tgtaccttgt atgggtatat aatagatatt gttttaataa aatatttgaa
      121 agggaatgat ttgatgaaaa ttactttacc agaaacagct attgatactt taaaaagtat
      181 cttaaaagac aatcaagata aacctaataa tataagagta tactttgcag gtgttggttg
      241 tggaggtcct tcttttggat tagctttaga tgagaaaaaa gaagatgact taacttatga
      301 agttggagaa ttacaatttg taatgagctc tgatgaatat agtcagtatg gagatataat
      361 aatagaagat acaggatttg gatttagagt tattcctgaa aatatgaagg atcaaggtgg
      421 aggaggatgc tctggttgtt cagggtgcca ctaagattaa gtttttagta agtttagaac
      481 tctctttata gaaataaatt tctattttaa gagagttctt tttatttttt ggtgagataa
      541 tgtataataa aaatgtattt tactaatttt ttatatgtaa ggagaattat tgtgagaaga
      601 agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata caaaatatgt tttaagagac
      661 gatatagaca agttaaaagg caagtggaat ttaaagttta gaaatgataa tccaatacat
      721 gttgaatttg ggactggaaa aggtaagttt ataacaactt tagctaagca gaaccctgac
      781 ataaattata ttgccatgga acttaaggaa gaagttcttt taaaggctgt tgaaaaagca
      841 gatgcttcta atttgaataa tatactattt ttatggggag atgtaagcaa tattttggat
      901 tactttgaag ctaaagaatt atcgaggatt tatataaatt tctgtgaccc atggcctaaa
      961 aatagatgga gtaaaagaag actcactcat tctggatttt tagaaatgta caatagggtt
     1021 ttggaagatg atggcgaaat tcactttaaa acagataatg aaaaattatt cgagtttagt
     1081 ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata tttctcttga tttaggaaat
     1141 agtgagtatg aaaataatgt tacaacagag tatgaagaca aatttatgtc acaaggtatg
     1201 cgaatattta gatgtgaagc taaaaaacgt aactagtaat atgtttataa gtttaatagt
     1261 agaaatttat tttaatttat attaataaag tgtacgaaag taggtatata taacaaggaa
     1321 acagaaccct ctcatagaaa ttaaattatt ataataaaaa atttatgtgg gagggtacta
     1381 tgaatttagc taatttaata ccatttatta tttctgcagc agctttaagt ccaataaatt
     1441 caaatgataa ggttttaagc gaaaatcaac ttaaagatat ttttcctgat gcaaatctaa
     1501 gagcagttgt aaaaagatat ataaacccag atgagatgac tataagtaat ataaaggctt
     1561 tagatggaga attttatgca actggtgaga gcatttcaaa tttaaaaggt atatcatatc
     1621 ttgaaaatgt agacaatttt attttttgga ataataatat aaaagaggtt cctaaggaag
     1681 ctttaagtct aaaagatatg gattcaataa atcttgccaa taattatttg attgatgacg
     1741 gtgtagtaaa ttctctttct aataatggtg tggatgtaaa ttgcgattta aattttattg
     1801 atacaaaaga taatcagtat aaactggact ctaaatatca taatgttgat atagcaaaag
     1861 gtgaaaatat tgatttgaga aaaataatta tgaagaaaat tgatagttat tataaatact
     1921 gggaagttac tgataatctt ccaaaagatt tagactttat tgtatctgta agtgataaga
     1981 gtgtactttc atgtgaagat atgataatta aaggtaataa aacaggtcaa gctgtggtaa
     2041 aagttatact aagcgacaaa aatgatttaa atacatctca agaagttcta atcactgtca
     2101 atgtaaaata atatgtcaac ttattcaatc attctatttt caatttaagt gtataatcaa
     2161 aataaaggtt gttagtaaca gaacgctaat attagtgctt tgttactagc aacctttaaa
     2221 ttttatagaa atagattatc taaaaaattc catttcttct gttagtagcc catattccta
     2281 ttaaaattat cagtgaatat attatataca aaactattct tatatactgg acatctaatc
     2341 caaaaaattc aatgttactc tgtgaggtat gcaacatcac aaggactgca gatataccaa
     2401 taagcaaagg attgattact atttgcttat cagttcttaa aatcattatt ttacccatcc
     2461 aaattattga taatataaaa aataaaattt taagaccaaa ttctaatgta tctaccaaag
     2521 cgaaaacatc ccttcattta taataaattt ttcagctcaa caaaatataa aaccctatga
     2581 acaactttta aatctataca aatttataat aagataactt gattaagcac tataaaataa
     2641 gt
//
LOCUS       DAEMAE010000331         2633 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEMAE010000331 DAEMAE010000000
VERSION     DAEMAE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.337"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            53..964
                     /locus_tag="KPH93_003582"
     CDS             53..964
                     /locus_tag="KPH93_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG1690345.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKTILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRCNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEACLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1139..1636
                     /locus_tag="KPH93_003583"
     CDS             1139..1636
                     /locus_tag="KPH93_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG1690346.1"
                     /translation="MNYEIVEISEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKSDKTIGLYTDYQGDFTSPYNFVACCEVDSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQGNTFDTNKQEIHI
                     YIGIK"
     gene            complement(1916..2275)
                     /locus_tag="KPH93_003584"
     CDS             complement(1916..2275)
                     /locus_tag="KPH93_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1690347.1"
                     /translation="MTHIYFNGQCKQAIELYKQAFNAIVKTLIEDSSNLLVVHAEIFI
                     HNELLILNDFGNKDGISISGGYQLCVQFDNETDLLKAYNTMKNDSTTIEPMQSTDYSS
                     CVVRFIDMFNVRWAFWV"
BASE COUNT         1049 a          250 c          441 g          893 t
ORIGIN      
        1 gtatgattaa tttagattaa ataaatttaa taattttagt aggtggtaat gaatgaaaat
       61 aaatagatta acagaaataa tagtaattct attaaataaa aaactagtaa cagctaaaga
      121 gttggcagat agatttgaag tttctacaag aaccatatat agagatatag aaactctgtc
      181 tatgtcagga gtaccagtat atatgacgaa aggcaaagga ggagggatat ctttaataga
      241 agaatattct atagacaaga ccatattgtc aaaaaaagat aaagagagtt taatagtggc
      301 actgaaaaca ttacaggcga ctaagtatcc agaaataaat tcagtagtaa ataaaattgg
      361 ctcaatattt ggagagcaga atttttcaaa ctggattgaa atagacttta ctgaatgggg
      421 aagtaatttt aatgaagatg ataaatttac aaaaataaaa gaagctattt taaggtgtaa
      481 tactataaat tttaattatg taaatagttt gtcaagccag acaaatagaa ctgttgaacc
      541 actgaagctt atgtataaga gtaaaacttg gtacttgtat ggattttgta aactaaaaga
      601 tgattttagg atatttagaa ttagtaggat aaggaattta tctataaagg atgaagtatt
      661 ttcaagaaag ataatagaag aagcatgttt gaatgattct aaagtaatta aggaaaatac
      721 tataacgttg aaattgaggt ttaaagaaaa gatgcttttt agagtatttg atgattttaa
      781 taaggattta ataaccaaaa atgaagatga tacatatgat gtaattacgg aatttcctat
      841 tggtgaatgg atttatggat atattttatc ttttggtgac aatgtagaag tacttgaacc
      901 taaagatgtt agggataatg taataactag actaagggag ttgtctaaaa tttattcatt
      961 ataatataat gctagattaa agattttatt aaatatataa tatctcgtat tgagtatata
     1021 atatctcact gagtttaatt atattgaaaa tatgacataa aggtgtcgta tattataaat
     1081 tataattgaa ttagttcaaa ataaaatata ttaataaaaa caaagtgagg tataaagtat
     1141 gaattatgaa atagtagaga taagtgagaa aatagttata ggaatatcga aggagactac
     1201 aaataaagat ggacaagcag taaatgatat aggtgaattg tggaaaaaat ttatgggcaa
     1261 agggatatat aatgccataa agggtaagaa gagtgacaag acaataggtc tatatacaga
     1321 ttaccagggt gattttacaa gtccgtataa ttttgttgca tgttgtgaag tagatagtaa
     1381 ttctgataaa gagaagaatt tagaagaatt aaacactaat aatactgttg gagaaagtat
     1441 tattagtaaa gttataccag caggtaagta tgcaaagttt gttatagtag gaggtcaaaa
     1501 agaagtagga gatttttggt ttgagttttg gcaaatggat tttgatagga cttatataag
     1561 tgattttgaa gagtatcaag gcaatacttt tgatacaaat aaacaagaaa ttcatattta
     1621 cataggaata aagtagattc gttattacta tattggatga agtattgagt atagtagatt
     1681 tattgtagat agttttatta tttataacta gaagcagtaa ctctgtaaaa gatttactgc
     1741 taagattgtt gacttatgat tttttgattt aataaaatca agaagatatt tgaattttat
     1801 agtagttttc attaagtgct aaaaacagca catacttttg ttgatgtatg tgctgaacat
     1861 ttatggtgtt ttgtatttca ctcaaatata taaagtgctt ataacatatt atagattaaa
     1921 cccaaaacgc ccatcttaca ttgaacatgt caataaatct aacgacacat gaactgtaat
     1981 cagtagactg cattggctca attgtggtac tatcgttttt cattgtattg tatgctttta
     2041 gtaggtcagt ttcattatca aattgaacac agagctgata tcctccagat attgaaattc
     2101 catctttatt cccaaaatcg tttaatatta gcaattcatt atgaataaat atttcagcat
     2161 gaacaacaag taaattactt gaatcttcaa ttaatgtttt aacaatggca ttaaacgctt
     2221 gcttatataa ttctattgct tgtttgcatt gtccattaaa gtaaatatga gtcaataaca
     2281 aaactatacc ccctaagcta atataaattt tttatactac acattatccg actaatgatt
     2341 atctgtatta taacatgatt aaatttaaaa agccatcaac acatagaatg tcaacggttt
     2401 gagcagtaaa tctgtaaaag atttactgct tttttagtaa ttaatagcat cagtatcact
     2461 ttgaatcttt agacatattc ttcatttaat attatttatt gataaatgat acttgtaaat
     2521 ataatgattt gttatttaat ttaaaatatg aaaagaaaag tattataaac acattagaac
     2581 acttctggat ttatgagtat acaatttgaa aaatataaaa aataatgtat aaa
//
LOCUS       DAEMAE010000332         2615 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEMAE010000332 DAEMAE010000000
VERSION     DAEMAE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.338"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1455
                     /locus_tag="KPH93_003585"
     CDS             <1..1455
                     /locus_tag="KPH93_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG1690348.1"
                     /translation="LLYPKNILLFIFVILIIPLVNTVLISGFIGKIQIPDYILDYIKS
                     DIVLNIIYMIFLLIIYILVIRWIFSIHEITLNTENFKRARKESVNLIKGKIIRTIIYS
                     LILSLVVAIIGYVIYHSGVIFIGIWTKYYTDMEFLKNIFINRCIAFEEYFRLILSIFI
                     FILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHVN
                     GMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLH
                     DSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIATLKEMIKYSK
                     GKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLT
                     CYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLD
                     LNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1776..2360
                     /locus_tag="KPH93_003586"
     CDS             1776..2360
                     /locus_tag="KPH93_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG1690349.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHRAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNVLI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1018 a          250 c          412 g          935 t
ORIGIN      
        1 ttgttatatc ctaaaaatat actattattt atatttgtga tattaataat tccattagta
       61 aatacagttt taatttcagg gtttatagga aaaatacaaa taccagatta catactagat
      121 tatataaagt ctgatatagt tttaaatata atttatatga tttttctttt aataatatat
      181 attttggtta ttagatggat ttttagcatt catgagataa ctttaaatac agaaaatttt
      241 aaaagagcaa gaaaagaaag tgttaatctt ataaagggaa agattataag aacaattatt
      301 tattcattaa tactatcttt agtagtagct attattggat atgtgattta ccatagtgga
      361 gttatattta taggcatatg gactaaatat tatactgata tggaattttt gaagaatata
      421 tttataaata gatgtattgc atttgaagaa tattttagat tgattttatc aatattcatt
      481 tttatattgg acattggatt tatctcagca acttattatc aatataaagg agtaagtatt
      541 tctaaaaata atattaaaaa agagaaaaaa tctatatcaa aaattgtttt taatttagta
      601 ttaatattat taatttctta tatagagtca gttgcatttt cttcacatgt aaatggtatg
      661 tttaatacgt cattttcgtt ttatacaaca gcaattgctc atagagcagg agctgaaatg
      721 gctccagaga atactttggc agcaatagaa gaagcttcac atgctaaagc tgattatgca
      781 gaaatagatg ttcaagaaac taaagatgga gaacttattg tacttcatga ttcaaacttt
      841 aagagaacta caggagtaga taaaaatgtt tgggatgtaa attataatga agtaaagaca
      901 tatgatgcgg gaagctttta ttattatgga aaaggttatt atgatgaaaa gtttgttggg
      961 gaaaagatag caacactcaa agaaatgata aaatattcta aaggtaaggt gaaattatta
     1021 atagaaataa aacttaatgg acatgaaaag ggagacgtag taaaaaaagt tgttcagtta
     1081 attaaagaaa ataattttga aaatcaatgt atagttgcat ctatggataa aactatatta
     1141 caaaaagtaa aaaaactgga tgagaattta ctgacatgct attttatggc aattgcatat
     1201 ggtgattttt caaaattaaa ttatgttgat atatatggaa ttgaatcaac ttttgttaat
     1261 aaaacagtag tagaaaaaat tcataaaatg aacaaaaaaa tcttcgtttg gaccgttaat
     1321 agtgatagtt taattgagaa aatgcttgac ctaaatgtag atagtattat tacagataat
     1381 ccatacctta tagagagtgc aatttattgg aagaaaaatg gatttattag tcaagtgtca
     1441 gattatttat tttaaatatt ctataattgt attatccagt aaaagagata ttattaaaat
     1501 caataatatc tctttttaac ataaacaagt ttgttaaatt aaggctattc ttataaaaat
     1561 tgtgcttatc agctataaaa aggaataaaa gtgaaaaaat tgccatgaca ttattacaat
     1621 taaaatagtt ttaatatgat agagaggtag attgtcaact gtatatttaa taaatcttgt
     1681 tatttattaa attttatata agtaaaatat atagatatat aaatcttatg ctagaatata
     1741 tatgaaaaac aatataaatt tggaggatta aaaacatgac agagcgagaa aaaatgttgg
     1801 caggagaact ttacgattgt ggtgatattg agctgttaac tcaatggcat agagcaaaag
     1861 acctagtaag agattataac caaacagatt cctcggattt ggaaaaaaaa gaaaaaatat
     1921 taaatgaatt acttggtggt aagggtaaaa acttatggat tactgcaccg tattatgtag
     1981 attatggaaa taatatttat tttggaaata attgtgaagt caatatgaat tgtacgtttt
     2041 tagatgataa taaaattatc attggggata atgtactaat tgcaccaaac gtacaaatat
     2101 atactgcatt tcatccaact aatgctggtg atagatttgg taatgccaaa gaagatggtt
     2161 catttgaatt ttgtaaaact caaacagctc cagttactat tggagataat gtttggattg
     2221 gcgggggtgc tattatcatg ccaggtgtta ctattggaga taatgtagtt ataggtgcag
     2281 gaagtattgt tacaaaggat attccaagta atatgattgc atacggaaat ccatgtcgta
     2341 ttattcgaga aaacaaataa agttaaattt tcttgcaaaa tctcaaaaaa cacctttctt
     2401 actttataca tagggtattt tccaatactt tcacatccat attcatttgt taaatttttg
     2461 tggaatttag tttttatcac ttctatctta catttccacc attttttata aaagtcatcc
     2521 ctttatagat ttatttataa tagtatatat tctatttgat gtttgaataa gcacttattt
     2581 ttaagtaaaa tactaacttt taggttagta ttttg
//
LOCUS       DAEMAE010000333         2613 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEMAE010000333 DAEMAE010000000
VERSION     DAEMAE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.339"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..587
                     /locus_tag="KPH93_003587"
     CDS             84..587
                     /locus_tag="KPH93_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG1690350.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENITRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEILRGEATE
                     CATKKSE"
     gene            634..1593
                     /gene="moaA"
                     /locus_tag="KPH93_003588"
     CDS             634..1593
                     /gene="moaA"
                     /locus_tag="KPH93_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG1690351.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVRFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1597..2067
                     /gene="moaC"
                     /locus_tag="KPH93_003589"
     CDS             1597..2067
                     /gene="moaC"
                     /locus_tag="KPH93_003589"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG1690352.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDIDDSISAIKIYATCK
                     SLGKTGVEMEALSACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2103..2534
                     /locus_tag="KPH93_003590"
     CDS             2103..2534
                     /locus_tag="KPH93_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG1690353.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENITTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVIKEGIINVNDSIDVI"
BASE COUNT         1084 a          239 c          518 g          772 t
ORIGIN      
        1 aaaagagaat aaaagaaagt aggttttatt aaaagagatt tttatttata ggacttaatc
       61 aatgtataga ggggtgagaa cgtatgttta atgttgcaat tataacactt agtgataaag
      121 gatatgaagg aaaaagagag gatataacag gaaaaaaatt aactgaattt gttgaaaata
      181 caggggctta taaagttact gaatatgtac ttataaaaga tgataaagaa atgctaaagg
      241 aaaatattac aagactttgt aatagtaata aaatagattt aattcttaca aatggaggta
      301 ctggtttttc aaaaagagat ataaccccag aagcaactaa ggaagtgtta gaaaaagaaa
      361 taccaggtct gagtgaatat atgagaatga aatccacaga aattactaaa aaggccatac
      421 taagtagggg tgtgagtgga ataagaaata attctataat aataaattta cctggaagtc
      481 ctaaaggtgc agttgaaaat ctaagtttta taattgatgt tttggaccat ggcatagaaa
      541 ttttaagagg agaagctaca gaatgtgcca ctaaaaaaag tgaatagtat ataatctaga
      601 tagataaaaa atagacaaag aggtagataa agtatgattg ataagtatgg aagaaaaata
      661 gactaccttc gtatttcact tacagataag tgtaatttaa ggtgtgtcta ttgtatgcca
      721 ccagatgtaa gatttgataa aaattatata aatgaaaatc taagctttga agattacaag
      781 tttattataa aggctatggc tgaacaaggt ataacaaaaa ttagatttac tggaggagaa
      841 cctcttttat atcctaaatt aaatgagctt ataaaattta caaaagaaga atgtggaata
      901 aataatatag gaatgaccac taatgctatt gggctttctg atagaatctt tgagcttgaa
      961 aaatgtggct taaataaagt taatataagt ttagattctt tgaaggaata taagtataaa
     1021 tccgtaacaa ggggaggaaa cttaaaagat gtcttaaaat cgattgaatc ttgtttaaat
     1081 ttaggtataa aagtaaagtt aaattgcgtt gctataagtg ggtttaatga tgatgagctt
     1141 aaagatttta tgtttttaac agttaaatca ccaattgatg taagatttat agagcttatg
     1201 ccattaggtg aggctaatag agtttataag aaggggtatt ttaatgtaaa agaaatgatt
     1261 gaaaaaatat atggtttata taaagtcaaa aatgatgaaa aaagtacagc agaatattat
     1321 atgtttaaag gagctaaagg aagaatagga attataactc cattaagctg ttcattttgt
     1381 aacacttgta atcgtattag acttacttct agtggtgcaa taaaattatg cctacattca
     1441 aaagaagaaa tagatataaa accattttta cataaaccat tgttatttag agaatctatg
     1501 aaagatataa taaagcaaaa accagaaaaa catcatttaa tagaaaataa atgtagtgat
     1561 acaaataggc gtatgtatga aattggagga taagtaatgt taacacatat aaatgaaaaa
     1621 gggtatgcca agatggtgga cataagtgaa aaagatgata ctaaaagaac ggctgtagct
     1681 acagctacaa ttactcttag taaggaagct ttagaaaaag taaaaaatgg tcagataaaa
     1741 aaaggagatg tattgtctgt agctcaagtt gcaggtataa tgggagctaa aaatacatct
     1801 tcaaacatac caatgtgcca tcagattaaa ttggttggat gtgatttaga atttgatata
     1861 gatgattcta tatctgctat taagatatac gcaacctgta aatctcttgg gaaaactgga
     1921 gtagaaatgg aagctttaag tgcatgttct ttagcagcac ttacaattta tgatatgtgt
     1981 aaagcagttg ataaaaaaat ggttataagt aatatccatc ttgtggagaa gactggtgga
     2041 aaatcaggag attttaagtt cgagtaaatt aataatatat tgggttaaaa tttggaggaa
     2101 atatggcgaa ggtagtgtca ataaatataa gtgagaaaaa gggtgtaatt aaagttcctg
     2161 tacaatgtgc agagctaaaa ataaatcatg ggattgttgg agatgcacat gcaggaaact
     2221 ggcatcgtca gataagctta cttggaaaag aaagcataga taaaatgaaa atgaaaggct
     2281 ttgaccaatt aaaatatgga gattttgctg aaaatataac tacagaaggg atagaagttt
     2341 tttccttgcc tgttggtacg aagttaagaa tagggcaatg tgaagttgaa gtaactcaaa
     2401 tagggaaaaa atgtcacaat ggatgtgaaa ttaagaaatt gacaggtgac tgtgtaatgc
     2461 cacgagaagg aatttttgta aaagttataa aggaaggcat tataaatgtg aatgattcaa
     2521 tagatgtaat ctaatgaagt cactattaaa atataattat aagtaaatac tattaagatt
     2581 taaatatata ttttaagaga gaattaattt taa
//
LOCUS       DAEMAE010000334         2612 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEMAE010000334 DAEMAE010000000
VERSION     DAEMAE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.340"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(285..893)
                     /locus_tag="KPH93_003591"
     CDS             complement(285..893)
                     /locus_tag="KPH93_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG1690354.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(1002..>2612)
                     /locus_tag="KPH93_003592"
     CDS             complement(1002..>2612)
                     /locus_tag="KPH93_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG1690355.1"
                     /translation="IKIDEEIRNINKDYESIILNIPSPILVRSAEGGIKKNKVISINK
                     KYEKSFKCASSDLVDMTLEQYFETFNIDFFDNRKFKRLNLTQEKKAEIVSYNDTANCI
                     VNFVMSDSEGEEHVEEVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLL
                     DVIPEAIFLEDLSTSRVLYTNRAFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGE
                     REKSIHIVNENNHIKEVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQY
                     DQMKMEFYANISHELKTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFR
                     LLRLIDNIINISQVKSDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEET
                     VMVGLDPESIERIILNILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKL
                     NDIFNRFKQIENSGISNEFGSGIGLCLTKSLIEIQNGKIYIDSKIGEGTNVKIIFPIK
                     EVVEEVYDNSNYNDNIEKFEIEFFDIYK"
BASE COUNT          846 a          428 c          198 g         1140 t
ORIGIN      
        1 gtcaaaattt cttcattctt taataattaa tttattactt ataaaataaa ctaattatct
       61 atacaaattt tataattttt aataaaatac acaaaaaaat taatttttat agctcatttc
      121 gccaaaatat atctttcttt atatcattaa aataattata aatttattaa atatgtttgt
      181 aattctataa agttattttt atcaaactaa aaattctata aaaaaataag atacatacct
      241 ataaaatacc ttcctataga tacatatctt acctatataa aaacttattc tatataaaaa
      301 cgccataaaa aatcttttgc ttcttctgca taatctatat ttatacgttt actagttttt
      361 atatctaact catctttact aaatactttt ttccctttat catcataata aaaatcactt
      421 atatataatt cttcacccat tatactctta ctatttagac ttctatctat ttttaaagct
      481 ttgcaaagtt ttcctggtcc attagtaata ttttttactt gatatttact caactcagtg
      541 taagctttgt tgtatctatt catagatatc tcatctaaag aagttatagg ctctacccct
      601 cttattaata cacactctgg tacattttct atatttgcag ataaatttaa gcaattatac
      661 ataccataaa ttaaataaac atatatatgt ccaccatcta aatataatgg cttagttcta
      721 tctgtctttt tattgccata tacatgtgct cctttatcat ttacacccat gtatgcttct
      781 gtctctatga ttttggtaac aattacttta ttttcatatt tccttattag atatttacct
      841 aatattgatt tggctaaatc aattccattt tttctaaaaa aatccttttc caaaatttca
      901 cctcataaaa tttatttgca acacatcaaa tacaactaaa gtaataaata aaattaactc
      961 ttattaatgt gatatatata aacaataaat ttagacattt attatttata tatatcaaaa
     1021 aattctatct caaacttttc aatattatca ttgtaatttg aattatcata tacttcttca
     1081 acaacctctt taattgggaa tataattttt acattagttc cttctcctat cttactatct
     1141 atgtaaatct tcccattttg aatttcaatc aatgattttg taaggcataa acctatacca
     1201 cttccaaatt catttgatat accactattt tcaatttgtt taaatcgatt aaatatatca
     1261 tttaattttt ctttgtctat acccactcca gtatctttta taattatctc tactgtttcg
     1321 tcttttttat atatacctac aaggatttct cctccttcta aagtaaattt tatagcattt
     1381 gataatatat ttagtattat tctttctatc gattctgggt caagccctac cataacagtt
     1441 tcttcatctg tatcaaaaat taaatctatt cctttagact tagcataact actaatagat
     1501 gtaactattc tttctgttat gtcaataata tcaaaattaa ctgctttaat cttatatatg
     1561 tctgacttaa cttgagaaat atttattata ttatctatta atctcaataa tctaaacata
     1621 ttttgtttag ttatttttat atgttctttt agtatatcct gaaaatcaat tatttttcct
     1681 tttttataaa ggttttcaat aagttgaact gaactatata tattatttaa aggtgtctta
     1741 agttcatgag atatattagc ataaaactcc atcttcattt ggtcatactg tctttgtttt
     1801 attaatgctt ttttaagtct ttcagactct ctttgcacac ttaaatcttt aataattctc
     1861 actctacttc ttttctgtcc aaagtataat gttcttgata tcatttttac ttctttaatg
     1921 tgattatttt catttactat atggatactc ttttctcttt ctcctatatt taaattgttt
     1981 atgtattttt ttactaatat atttctatat ttttgtgttg taactcctaa tacatcacta
     2041 gaaattccga acaactctct aaaggctcta tttgtatata gtacccttga tgtgcttaaa
     2101 tcctcaagaa aaatagcctc tggtataaca tccagtaact tttcatatat tattttttgt
     2161 tcatttactg ttctaagaat atttatctct ttagttatat ctctaaatgt aagcaattta
     2221 aaaagtttat tatctgacca atagtctcca actctaactt cttcaacatg ctcttcacct
     2281 tctgagtcag acatcacaaa attaactata cagtttgcgg tgtcattgta acttactatt
     2341 tcagctttct tttcttgtgt tagatttaat cttttaaact ttctattgtc aaagaaatct
     2401 atattaaaag tttcaaaata ttgttctaat gtcatatcga ctaaatcact tgaagcacat
     2461 ttaaaactct tttcatattt tttatttatg cttattacct tattcttctt tataccacct
     2521 tcagcacttc ttacaagtat gggagatgga atatttagta taatgctttc atagtcttta
     2581 tttatatttc ttatttcttc atctattttg at
//
LOCUS       DAEMAE010000335         2583 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEMAE010000335 DAEMAE010000000
VERSION     DAEMAE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.341"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(309..1076)
                     /locus_tag="KPH93_003593"
     CDS             complement(309..1076)
                     /locus_tag="KPH93_003593"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG1690356.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIPDIPLLFTFRSVVEGGAKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELSADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            1422..1643
                     /locus_tag="KPH93_003594"
                     /pseudo
     CDS             1422..1643
                     /locus_tag="KPH93_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725701.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1883..2515
                     /locus_tag="KPH93_003595"
     CDS             1883..2515
                     /locus_tag="KPH93_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG1690357.1"
                     /translation="MTIPNLNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSNSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGIHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          979 a          382 c          271 g          951 t
ORIGIN      
        1 gacaaatata cataatacaa atatatcttc tactttatat aaaacagtta ttatgaaaac
       61 ttactatcta taaataatag aaatgctagt ataccatcta taatctaatg tctagtaggt
      121 tttaatggca tattttaaat atgccatagt tgatttataa gattttctat atcactttca
      181 aaatacacac taaatagttg cttttcagat ataattgccc aatttaaatt ttactatctg
      241 aaaagttttt tatatttaat ttatatcttc aaaaattaaa taccttctaa tatttctata
      301 taattaaatt aattaatact cttatgtaat aaattaagta ctgaatttaa ctctttaaat
      361 gatatttgtc ctggcgctga aacacttttt gcagctccaa aagtcaatgc agaaccaaat
      421 atttcaccac acaatctact tataacgccc attcctgaca ttgacattgt tattattggt
      481 ctatctgcat aaattttaaa catttcattt gtagcttcaa gtagcactaa cacatctttt
      541 tcattttgcg gcataacagc tattttgggt aaatctgcgc tcaattcctg cattctacat
      601 aaacgagata caatctcttc tttttttgga gttttattaa aatcatgatt agatataatt
      661 actttcactt cttttttatg agcaaaatta actacttcat ctataacctc atcccccata
      721 aataactcta catctattaa atctactaaa cctgtattag aaatttcttt attcaaagta
      781 gtataataat ctcttgatat taattttgct cccccttcta ctacacttct aaatgtgaac
      841 aataaaggga tatcaggtat ataacttctt aattcataga gaacttcctt aacttcttta
      901 atattttcta cattctcaaa aaaatcaact cgccactcta taatatccaa acatgcatct
      961 tttaactctt tagcctcttt tataatatct tttttatttt tcccgataat tggtacacaa
     1021 attttaggtc taccttctcc aatagtaata ttttttactt gaacttttct tttcatattc
     1081 tcctccataa tcagaaattc tataggactt taaattttat tttctacaca atttaattat
     1141 acattataaa tactatacat tataaaaata ctaatttaaa agtaattttt attctaattc
     1201 ttaaaacaat cccacatatt ttagaaaaga tactctataa tatacaaaca cttatttctt
     1261 acctaaattt attttaacat tctataattt tcacaatatt ccttaaattt tactcatata
     1321 atcaatatta ttctgcactc taatagaatt aatctataaa tcatagtata attatacaga
     1381 aataactcga atttcaccac ttatgaaggg atttaatatt aatgaatcta aatcatttgc
     1441 attacttcag agttttagct aaagttgaac actatacaaa agcagctact caattgtcta
     1501 taccccaacc aagcttaagt aatgctatat agattctcga aaaagaattg agtacttttt
     1561 tatttgaaaa acaaggtaga aatgtttgcc tcaccaaata cggaaggttt ttcctaacct
     1621 atgttgataa agcattaagt taataaaaaa aagttgcata taaaatatat taaattgttg
     1681 caagttttat tgttttgata tacaatcaac taaacatata atctttataa aatactatac
     1741 agttaaaagt ctgtttttaa aaacaaaatg tataaattat aaattaaaat atagatacta
     1801 tcaaggagtg attctgtgaa gagatgtatt gtagtagtgg aatagtcaaa aacataatat
     1861 atatcaaagg agagatttaa atatgactat tccaaattta aataaggtat atccaagaag
     1921 taatgattat caaactgtat atcttaaaaa tgtaataaca agagaaaata tagaagttgg
     1981 agattacaca atatacaatg acttttataa tgacccaaga gattttgaga aaaacaatgt
     2041 attatatcat taccctataa acaatgataa attaataatt ggtaagtttt gctcaatagc
     2101 atgtaatgca aaatttctaa tgactagtgg aaatcactca atgaaatcaa actccacata
     2161 tacctttccc atcttttatg aagaatggaa actcgatatc aaccatataa cggacgcttg
     2221 ggacaataaa ggtaacatta taattggaaa tgatgtgtgg ataggttatg attctataat
     2281 tatgtcaggt atacatattg gtgatggtgc aattattgga acaagagcag tagttactaa
     2341 tgacgtccca ccgtattcta ttgttggagg agtacctgca aagattataa aaaagagatt
     2401 taataatgat acaatttcaa aattattaga aattaaatgg tgggattggc catataaaaa
     2461 aattcaatca aacattaagc atattcaatc aggaaatatt gataaattag tttaatattt
     2521 tataatttat ttatattaaa ttttacttta ataatccttt tataaaagct ttttcaaaat
     2581 ttg
//
LOCUS       DAEMAE010000336         2519 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEMAE010000336 DAEMAE010000000
VERSION     DAEMAE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.342"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            72..545
                     /locus_tag="KPH93_003596"
     CDS             72..545
                     /locus_tag="KPH93_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1690358.1"
                     /translation="MFKEYLIRELKEDNIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYKSIIEKVKEKTLFFWGCFDSKKLIGVLAIRDLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDSIRFTPMKLLI"
     gene            complement(904..1461)
                     /locus_tag="KPH93_003597"
     CDS             complement(904..1461)
                     /locus_tag="KPH93_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1690359.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNNQMIKAVIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1724..2422
                     /locus_tag="KPH93_003598"
     CDS             1724..2422
                     /locus_tag="KPH93_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1690360.1"
                     /translation="MEKKYFKIHGVPSILWGNPSSKLYIYIHGQGGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKGEKNSFDPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQKLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPIIQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIMENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          938 a          310 c          316 g          955 t
ORIGIN      
        1 gaattaaatt tatttaagat atataattat aattataatt aaaattttta ataataaagg
       61 tgttgtttat aatgttcaaa gaatacttaa taagagaatt aaaagaagat aatattaaaa
      121 atgcattaga cttaatctgg aacgtatttg aagaatttga atctccagat tattgtgatg
      181 aaggaattac gacatttcat caatttatag aatataaatc tattattgaa aaagtcaaag
      241 aaaaaacact cttcttttgg ggttgttttg actctaaaaa acttattgga gtcttagcta
      301 ttagagattt aaatcatata tgtatgttat ttgtagataa aaactatcat aaacatggtg
      361 ttgcaaaaaa attaatgact acaggtatac aatactgtat ttgtaataaa tttaatttaa
      421 ctgaaataac tgtaaattca tcaccttatg ctgttgaagt ttatcataaa ttaggattta
      481 aagatactgc tgttgagcaa attgttgaca gtatacgttt tacacctatg aaattgttaa
      541 tataataaat taaataaaaa gcaaagtctc ttaaatattt tatagtgact ttgcttttaa
      601 accgagattt aaaaataata ttatcttttt tattttttaa taatatactt taaaaacatt
      661 actaatatga actctaattt atttttgata tcatttttga ctaacatata aattaattca
      721 attattaagc ataaactatg ttatttctct tatttgtaag caatattggt catttgaaaa
      781 taaatattct taaaatatac tgttctcctg tggattttcc tttggtataa acgataaaat
      841 tttaatttac atgccttgta aagaagtgtt caataaataa gttaaaataa tttatttact
      901 tccttaatca tattttttat gaatacctaa taacacagta tcaatagaat cggctaaacc
      961 ttttatagct tcttctttac tcaaaagtga tttcccaagt tgcaaagtcg aataaccatg
     1021 aagtacactc attattaagt ttaatacttc acctgtattc tttgtattta gattacaaga
     1081 aagaataagt ttttctaata atgatttata ttcatcaaag atttctaatg tttcattatt
     1141 tccatgccaa gtagcccact gaattgtttc ataaacacca ggattagaaa ttataaaatt
     1201 aaagtatgct atacttatac actttatagc tgtatctcca aaatttccaa taaccgcctt
     1261 tatcatttga ttattcatct ctctcattcc tttatgagct acttcaagta ataaatcatc
     1321 tagactttct atatgattgt acaaagatgg tgtacgtatt cctagttttt ctgcaataac
     1381 ttttaaagaa actttgttaa gcccatcttt attaactata tctgatgtag cttgtataac
     1441 tatttctcta ctgacacgca tgattttccc tctttcattt aaaattgatt actttattag
     1501 tttagctaat ctaattagtt tcgtcaatta ttgtaattta taaaattctt tatactatta
     1561 taaaatcttg acagtttaat tataatttaa tataatgcta attgaattag ttttaatact
     1621 attataatta gtattatatt atattaaatt atatattaca acattatttt atagtttgat
     1681 taatttaaca ataactaata tatcaaactg agaggagatt tttatggaaa aaaaatattt
     1741 caaaattcat ggtgttcctt caatcctttg gggaaatcct tcaagtaaat tatatattta
     1801 catacatgga caaggtggca ataaagagga ggctgaagct tttgctaata ttgcagtccc
     1861 acatgagtgt caagttttaa gtgttgattt acctgagcat ggagagcgta aaggagaaaa
     1921 aaattcattt gacccatggc atgtggttcc agaactagta tctattatgg aatatgcaaa
     1981 gtgccactgg aatcaaattt cgttatatgc aaatagcatt ggagcatggt ttagtatgtt
     2041 aagctttgaa aaagagaact taagagagtg tatttttgta tctccaattc tagatatgca
     2101 aaagcttatt tcaaatatga tgctttgggc aaatgtatca gaagaacagc ttaaacatga
     2161 actaattatc ccaacttcat ttgggcatac tctttcatgg gaatatctac aatatgtaaa
     2221 acaacatcct atcatccaat ggaatgtacc aacaaaaata ttatatggta aacatgatga
     2281 gcttactgaa atcggtgttg tagagaaatt tgtagaaaag tttaattgca accttactat
     2341 tatggaaaat ggaaaacatt ggtttaacac accccaagaa ttaaattcat tatatgaatg
     2401 gttaaatact ttagttaaat aaaaaataat tatattatta atatgattaa attatttata
     2461 aatgttattt aattaatttt attttagtat tattaaaata actacataaa gaacaaatt
//
LOCUS       DAEMAE010000337         2492 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEMAE010000337 DAEMAE010000000
VERSION     DAEMAE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.344"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            295..1566
                     /gene="serS"
                     /locus_tag="KPH93_003599"
     CDS             295..1566
                     /gene="serS"
                     /locus_tag="KPH93_003599"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG1690361.1"
                     /translation="MLDIKRIRENLDGIKKAMERRGEKDFNLDEVVKLDDERRRILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKQLSDKIKIIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVIVPEVLRPYMGVSVIK"
     gene            1891..>2492
                     /locus_tag="KPH93_003600"
     CDS             1891..>2492
                     /locus_tag="KPH93_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1690362.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYG"
BASE COUNT         1056 a          245 c          430 g          761 t
ORIGIN      
        1 aaaagttaat aactatacaa gattgataaa gaaaaagtca aaatagataa ttgattagca
       61 aaatagatat aattataatg aagttaaatc tagcaatatt ctcctaaaat taaattatat
      121 ttatttaatt aaatataatt tgacaataga caaaaaagtg agtaaaatat aaataaaata
      181 aaaataacag gttcggataa agaaaaaggt ttcttaacct caagcataaa gtatattatc
      241 taagttattt gagattaaga gataaatcca atcttaggta ttgaggagga aaaaatgctt
      301 gatataaaaa gaataagaga aaatttagat ggtataaaaa aagcaatgga aagaaggggc
      361 gaaaaagact ttaaccttga tgaagtagtt aagttagatg atgaaagaag aaggattctt
      421 caagaagttg aagttatgaa aaatgaatta aatacagcat ctaaaaatat accaaatctt
      481 ataaaagaag gtaaggatgt agaaaatgaa aagattaaac taaaacagtt atctgataaa
      541 attaagataa tagaccaaaa tcttaaagaa gtagaagaca agatggaata cctattaatg
      601 agaataccaa atgtaccaca tccagaagta ccacaaggtg aaactgatga agataatgtt
      661 gaggtaagaa cttggggaaa gactactacc tttgattttg aatctaaggc tcattgggaa
      721 attggaacag aattaggtat acttgatttt gaaacagcta gtaaaataac tggttcaagg
      781 tttactcttt acaaaggatt aggtgctaga ttagagagag cattattaaa tttttacttg
      841 gacacaaata caaaagtaaa tggatataca gaagttattc caccatttat ggcaaataga
      901 aatagttttt taggtacagg acagttacct aagtttgaag aagatatgtt taaaatagaa
      961 ggtctggact attttatgat tccaacatca gaagttcctt tgactaatat acatgctaat
     1021 gaaatactta aatttgaaca gttaccaata aattatacag cttatactcc atgttttagg
     1081 tcagaagctg gttctgctgg aagagataca agaggtttgg taagacagca tcagtttaat
     1141 aaagtagaaa tggttaaaat agttgcacca gaagaatctt acaatgagtt agaaaagctt
     1201 actaacaatg cggaaactat gctacaatta ttgaatctgc cttatagagt agtaaagata
     1261 tgtacaggtg atttaggatt tacggcttct tttaaatatg atgtagaagt ttggatgcca
     1321 agttacaata ggtatgttga gatttcatct tgttcaaact gcgaagattt ccaagctaga
     1381 agagcaggaa taagatttaa aagagataaa gattctaaag ctgaatatgt acacacatta
     1441 aatggctctg gtctagctat tggaagaagt gttgcagcta tattagaaaa ttatcaacaa
     1501 gaagatggtt ccgtgattgt tcctgaagtt ttaagaccat atatgggagt ttctgttatt
     1561 aaataagagt taaattatgt atttttaagt aatacaataa tatagaggta ataaaaaaat
     1621 aattaagata gaagttgtac aataagatag aagtaataaa aaaattaata taaaaatgat
     1681 acaataataa tagaagtaat aaaaaaaata attaatataa aagcgataca ataatagtat
     1741 aagtaataaa aaaaaataat taatataaaa atgtgtacaa actaatgaat attgatatag
     1801 taagctatca tatgaaagag tatgtacaca tttttgttta ctaatttttt acaatatgaa
     1861 cataaataat ttacaggaaa ggaatctttt atgaaaattc aaaatactga gtggggatat
     1921 attgaatgga aacatactta tgatgaaaat aatccaaaac aagctatgaa tatatacatt
     1981 gcagtaacta tgcctggtaa gaaacatttt aatcatgttc attatggtca agagcagatg
     2041 atatatattt tagaaggaga gggtttatat attataaatg gagtttggaa gccattttat
     2101 caagggatga ttttttatat agaatctggt agcacacatg aaactataaa tacaggcgat
     2161 agagaaataa aagaacttat tgtttcaaat aatgtagatg atgtaggaga gagtgaagtt
     2221 atagatatta atcctaataa ttatttaaag aagactttga taaattatag tgaaagcaca
     2281 ttaaatttgt atgcagcggt agaatcaatt cgtggtcaat ttatagaccc atttaaaatc
     2341 ccattaatta tatatgatga ttcatggaat atagttttaa aaaaccctta ttttccactt
     2401 ttttgttttg aaaaatgtaa tcctatgaaa tttcctcaaa attgtgattg tatgaatcaa
     2461 aaaagtagca atcaatttgt atgtgaatat gg
//
LOCUS       DAEMAE010000338         2456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEMAE010000338 DAEMAE010000000
VERSION     DAEMAE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.345"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..168)
                     /locus_tag="KPH93_003601"
     CDS             complement(<1..168)
                     /locus_tag="KPH93_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG1690363.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHTDIKITMNRYSQVLEEM
                     DREAFENLSKIL"
     gene            424..582
                     /locus_tag="KPH93_003602"
     CDS             424..582
                     /locus_tag="KPH93_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690364.1"
                     /translation="MVCKIFITLKAILLKTQIIVTTHSPHILQIDSKEEIIMLDIDES
                     GNVYKKEL"
     gene            637..801
                     /locus_tag="KPH93_003603"
     CDS             637..801
                     /locus_tag="KPH93_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690365.1"
                     /translation="MGIERNFLKLYLKVIINFEDAEKVKKNYEILDKMLHPKNHLRKM
                     LQIQMAGFMK"
     gene            959..1153
                     /locus_tag="KPH93_003604"
     CDS             959..1153
                     /locus_tag="KPH93_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690366.1"
                     /translation="MNHYHPKSKYPYEVLVWENLLFSCGMCNNKKRAYDTDLEPIINP
                     TEYKPKEILTLAKFFICKQK"
     gene            1200..1520
                     /locus_tag="KPH93_003605"
     CDS             1200..1520
                     /locus_tag="KPH93_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690367.1"
                     /translation="MNELVRHRFKIYLCLKKIISSVVILLGNYEKSINNSIIYGNKIV
                     SSIKDLLKISQLTKEYNSAMSTFLLTDLDYKYIKEMMLKFNLLDDELSELESTVKEIM
                     LDSE"
     gene            complement(1538..1972)
                     /locus_tag="KPH93_003606"
     CDS             complement(1538..1972)
                     /locus_tag="KPH93_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690368.1"
                     /translation="MESMNSFKKIEIEHVRIRVQLEKNNPSTALYISLLAVLFSFLYN
                     FAFETIKTTLNGLITVLKIENLDINLIANVICFVSFLFFVQNMSSKIGSLCRNDIQFY
                     ITALDLLEDMKKKNLEKESIKKEDNKEIFITFFFYTLKFNCI"
BASE COUNT         1012 a          231 c          320 g          893 t
ORIGIN      
        1 tagtattttg ctcaaatttt caaatgcttc tctgtccatt tcttctaaaa cctgagagta
       61 tctattcata gttattttta tatctgtatg acctaatctt tctgaaatag tttttatatt
      121 agttccagaa agaaacatta aagttgcatt attatagaat ttaaacataa ttaaatgtaa
      181 aaatacagtt aaaaattaat tgaaaatatt aattatatgt tatgatataa taaaaatata
      241 gaaattttgc agtgagcgat atttgtgaca aatttgaggt ttaacagttg aaatataagg
      301 cattgagaat gtgtgataag tattatcaat tgcactattg cacgctcact gcaattttaa
      361 aagcattgta tatgtgtaaa cattggagat gctaagttta ttttgaggtt ttatattaac
      421 tatgtggtat gtaaaatttt tattacatta aaagcaattc tcctaaaaac acaaataatt
      481 gtcacaacac acagtcctca tattttacaa attgattcta aagaagaaat aattatgtta
      541 gatatagatg aaagtggtaa tgtatataaa aaagagttat aacttggaga atatgggtta
      601 ttaggctgga ctaatgaaga aatattaaaa gatgttatgg gtatagaacg taatttttta
      661 aaattatatc tgaaagtgat tataaatttt gaagatgctg aaaaagtaaa aaagaattat
      721 gaaattttag ataaaatgtt acatcctaaa aatcatctta gaaaaatgtt gcaaatacaa
      781 atggcaggtt ttatgaaatg ataaaaatag aaagaaaacc taaatccaaa caattaacag
      841 aagagtataa aaaaacgtat ggaataaaaa atatataaaa aatacacttt ttgtcatatc
      901 taatggaaaa tgttgctact gtgaaactaa attaatggaa gaaggcaaat acatgtaagt
      961 gaatcattat catcctaaaa gtaagtatcc atatgaagta ttagtatggg agaatttatt
     1021 gttttcttgt ggaatgtgta ataataaaaa acgtgcatat gatactgatt tagagccaat
     1081 tataaatcca acagagtaca agcctaaaga aatacttaca cttgcgaaat tttttatatg
     1141 taagcaaaaa tagaaattaa ttaggcgagt gtactattga tgtattagaa ttaaataatg
     1201 tgaatgaatt agttaggcac agatttaaaa tatacctatg tttaaaaaag ataatttctt
     1261 ctgttgttat tttattagga aattatgaaa aaagtattaa taattcaata atttatggaa
     1321 ataaaattgt aagttcaatt aaagatttat taaaaatatc tcaactaaca aaagaataca
     1381 actctgctat gtcaacattt ttgttaacag atttagatta taaatacata aaagaaatga
     1441 tgttaaagtt taatttattg gatgatgaat taagtgaatt agaaagtaca gtaaaagaaa
     1501 taatgttaga ttcagaatga aatacatatt aaaataacta gatacaatta aatttgagag
     1561 tataaaaaaa gaaggtaata aaaatttctt tattatcttc tttttttata ctttctttct
     1621 ctaagttttt ctttttcata tcttctaata aatctagtgc agttatataa aattgtatgt
     1681 catttctaca taaagatcct atcttagatg acatattttg tacgaaaaac aaaaaagaaa
     1741 caaaacatat aacattagct attaaattta tatctaaatt ttcaattttt aaaacagtta
     1801 tcaaaccatt taaagttgtt tttatagttt caaatgcaaa attatataaa aatgaaaaaa
     1861 gtacagctag aagagatatg tacaatgcag tagaaggatt atttttttct aactgaactc
     1921 taattctgac atgttcaatt tctatttttt taaaactatt catactttcc atttcattat
     1981 aataatagat ttttgtgtaa tatttatggt atttcttaaa atatttttta tctttcagat
     2041 tttgaaacta gttcactaaa tttataatta ctaaaatcat aaaatactcc gagtatatat
     2101 aatatacttt aatttatagc atatttaaga acttatatta gcaatttgtc gaacgatttt
     2161 agataaaata gagctagttg agaaaaactt actcaaatat atgagcaatg atgtatggcc
     2221 tgcaatggat aaggctagag agtttgtaaa aacaagctat aagttttaaa tttttattta
     2281 aacaagtatg cagataataa aaatttatat aaaatttttt cttgtaaata tatttattaa
     2341 aataattaaa tttataaaag cgtatatttc tagaaaaaaa tatatttttt gaccatttat
     2401 acatttttaa tgaccagtta gacaataagt gttgaatatt ttgaatattt gattta
//
LOCUS       DAEMAE010000339         2451 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEMAE010000339 DAEMAE010000000
VERSION     DAEMAE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.346"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..2434
                     /locus_tag="KPH93_003607"
     CDS             191..2434
                     /locus_tag="KPH93_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1690369.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSHFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     DGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLADGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSTGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGYVVFQGSYDKLLLANTLTSNAMNLSLPIKTNSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPI
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDEALEFFTEKKLCKILQTMKDV
                     GLPYITLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSIKI"
BASE COUNT          907 a          337 c          416 g          791 t
ORIGIN      
        1 agtatctatt atatactttt ttatctacta tcaactacta tttattcaga ttgtatggga
       61 ttggttaaat ttagaaatac tttctataac atagcagaat atatcaagaa agtagatttt
      121 aattttatta taatatggtt gcataatata attgctttac taaaatgtac tcagaaagga
      181 tttaacattc atgtcaaata ttataataaa aggacttaca caaaataacc taaaaaatat
      241 atcatttaaa attcctaaaa ataaaattgt tgtttttact ggagtttctg gctctggaaa
      301 atccagtatc gtatttgata caattgcagc agaatcacag aggcagatga atgaaacata
      361 ttcttcattt atcagaaata ggctacctaa atatagaaaa cctcaggtag agctgattga
      421 taatttaact gcttctgtaa tcgtagacca aactcccctt ggcggcaatg cacgttctac
      481 agttggtact ataagtgatt tgtattcatc attaagatta ttattttcta gaattggaga
      541 accctacata ggaacaactt cacatttttc ttttaatgac ccaaatggta tgtgtaaaaa
      601 ttgttctggg attggtagag ttaaagtaat caatattgaa tctattataa atcctgaaaa
      661 ttcatggaat acaggatgta taaatgactc tttatttaaa cctaattcat ggtactggaa
      721 gcagtatgca gagtctggac tttttgattt agataaacct ataaaagatt attcttttga
      781 agaatataat ttattgcttt atggttcaaa aaataaagat ggaaaacaag aaaatccaaa
      841 aatagatggt atttttaaca aatacaatca aacatatcta aatcgggata ttagtaatat
      901 gagcaaacac acaaaagaaa aatcagaaag acttataaca gataaagaat gtcctgtatg
      961 tcatggaaaa agattaaata aagaaactct taactgtaat attttaggat attcgattgc
     1021 agatatgtgc aatatggaac ttactgacct ttatgattta ttgttacaaa taaaagataa
     1081 aagaactgaa actgtaataa aaactcttgc tgatggttta aaaagaatga ttgatattgg
     1141 actcccttac ctacatctaa atagagaatc atcatctcta tctggaggag aagctcaaag
     1201 attaaaactt gtacgctata tggggagtag tctcacaggt atgacttata ttttcgatga
     1261 acctagtact ggtatgcatc cacgagatgt acatcgtatg aatcagttat taaaaaaact
     1321 tagagataga ggaaacactg tccttgtagt agagcatgat aaagatgtta tctccatagc
     1381 agatgaagtt atagatattg gcccaagtgc tggcacacag ggtggatatg ttgtattcca
     1441 aggctcttat gataaattac ttttagcaaa cactcttact tcaaatgcaa tgaatctatc
     1501 attacctata aaaactaact ctcgtcatcc aaaaggtacc cttccaatta gagatgcttg
     1561 cataaataat ctaaaacata ttgatgtaga tttaccagtt ggaattatga ctgttgttac
     1621 tggagtagca ggttctggca agagtacttt aatctctcgt gtttttgcac aaaaatacga
     1681 aaaagatgtt attaaaatcg accaaagccc aattacagct acaaaccgtt ctatgcctgc
     1741 tacatttctt ggtttctttg atgagataag aaaagtattt gctaaagaaa ataatgttga
     1801 agaaggttta ttcagcttta attcaaaagg agcttgtcct gtttgtgatg gtaaaggagt
     1861 acttgttaca gaacttgtat tcatggaccc tattattaat acatgtgaag cttgcaaagg
     1921 tactagatat agtgaagaag ttttgtcaaa attatataaa ggcaaatgta tagttgatgt
     1981 actatctatg acagtagatg aagctcttga attttttaca gaaaagaagc tatgtaaaat
     2041 cttacaaaca atgaaagatg ttggtttacc ttatataacc ttaggtcaac cacttagtac
     2101 actatctgga ggagaacgtc aacgaattaa attagctaaa aacctcaaca aaaaaggtac
     2161 tatatatatt ttagatgagc cgactacagg gcttcatggt tctgatattg aaaagcttat
     2221 gttgattttt gagcatatag ttgataaagg taatactgta attataattg aacataattt
     2281 ggatgtattg aaacaagcag attgggtaat tgatattggt cctgatggtg gcaaaaatgg
     2341 tggtcaggtc gtatttgaag gtacaccact agaaatgttt gaaagaggtt gcactataac
     2401 atcagatttc cttagaaaat caataaagat atagataata taatactaat a
//
LOCUS       DAEMAE010000340         2421 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEMAE010000340 DAEMAE010000000
VERSION     DAEMAE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.347"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(109..960)
                     /locus_tag="KPH93_003608"
     CDS             complement(109..960)
                     /locus_tag="KPH93_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG1690370.1"
                     /translation="MALVTTTSMLKKAQDGGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISLSKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAVAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDNIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLNEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1009..2178)
                     /locus_tag="KPH93_003609"
     CDS             complement(1009..2178)
                     /locus_tag="KPH93_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1690371.1"
                     /translation="MKMFKLEEQKLKDLGAIITTKEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNNIRKYIFEAIPTTDIVSNP
                     YDYLKKDVPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLMFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKIATVYDSPMGFRHGPK
                     SFIDEKTLVFEFVSNCSYTRKYDLDVLEEIKRDKIAKFTCAVSVENESNFSGTKFEFI
                     GKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
BASE COUNT          740 a          449 c          264 g          968 t
ORIGIN      
        1 tttcatcaat taaatgaaaa aaatacacca tttacctata attaatgtaa aattttcata
       61 taacaaatat cctaaactta tctaaaataa atttaggata ttttattttt atactctatt
      121 tacagaccca cacactttaa tcttttcttt tacaaaatcc tttactgatt gaagccctgc
      181 tagtccatac tttttagggt ctataacctc agaatcttca tttaaaactt ttttaacccc
      241 tttactatat gcaattctta attcagttgc atagttaact tttgcaatac ctctttttat
      301 agcttctttt ataatattat catgtattcc tgaacctcca tgcaatacta aaggtattga
      361 aactacattt ttaatttctg ataacctatc taaatctatt ttaggttcac ccttatacaa
      421 tccatgtgca gtacctatag caacagccaa tgaagatatt cctgttctat cgacaaactc
      481 ttttgcctct tttggatttg tataatcatt actattcccg ccatctaaat catcttcttt
      541 tccaccaact ttcccaagct ctgcttccac tggtataaga gaaggactac atgcttttac
      601 tactgattta gatagagata tattattttc aaaactttca tgagaaccat ctatcattat
      661 tgatgtatat ccacttctta aagctttcat tgcaaggtca aaacttgaac catggtccag
      721 atgcattgct acaggtatct ttactctttc tgccgcaacc tttgcatttg ctagataata
      781 atcgagacct gcatatttta cagtagatgg agtagtttgt aatattactg gagaatttaa
      841 ctcctcagca gcttctataa cagcaattac catttccata ttttccacgt taaatgctcc
      901 tactgcatac cccccatctt gtgccttttt aagcattgat gttgttgtta ctagtgccac
      961 attgataccc tcccaaatta tttagttata atattttaat cttctctttt aatattcata
     1021 tattgttaca ccttttacta ctcgatttac tgttcctgta gctgatggcg tatctggctt
     1081 atttccaacc tttaccgata caaacaatgc tatagtttgt gcaaacaata tgtatggcat
     1141 tgcaagatat acatctggta atttattata ctttcctata aactcaaact ttgttcctga
     1201 aaagttactt tcattttcta ctgacaccgc acaagtgaat ttagcaattt tatccctttt
     1261 tatttcctct aaaacatcta aatcatattt tcttgtatat gagcaattag atacaaattc
     1321 aaatacaagt gtcttttcat caataaatga ttttggtcca tgtctaaacc ccataggaga
     1381 atcatatact gtagctattt ttcctgctgt aagttctaaa agttttagtt gtgcttcttg
     1441 tgtcaaacca cctaatcctc ctgaacctaa atatacaact ctatcaaaat ctttatttat
     1501 taactcatgt atttcatctt ttctatctat aacatttctt cccatttcaa ttattgcatt
     1561 tatatatgac ttttctacat catcttctaa actatcgaac attaacatag cagataacat
     1621 catacacgaa aaacttcctg tcatagcaaa gccttcatca tttgattttg atggcattaa
     1681 taacaagtaa ttattttcgt cgtttttagt catcttagcc agttctcctt ctgggttgca
     1741 agttatagct agatgataaa aattatctac aatcttattc cctaaattta gagcagccaa
     1801 actttcaggg ctatttccac ttcttgcaaa tgaaattaat aatgttggta catctttttt
     1861 taagtaatca tatggattag aaactatatc cgtagtaggt attgcttcaa atatatattt
     1921 tcttatgtta tttttatttt taaggtaagg aagtattgag tttcctatgt aagctgaagt
     1981 tccagcacct gtaaatataa ctctaaactg cccatgatta ttacttattg tatctatgaa
     2041 tctacttaat ttttctttgt tagatttata tatttcataa gtctctaacc ataattcagg
     2101 ttgttgtttt atttctttag ttgttattat tgcacctaaa tcttttagtt tttgttcttc
     2161 caatttaaac attttcattc tcctttcata cttgtaacgt ttagatttgg taatgaggtt
     2221 ataaccactt taaattataa aaaaatatac tcttttgtaa ttttttactt agatttttta
     2281 gcactactta aactcactat aaaatatacg ctatagattt taattaattt tatagtttat
     2341 ctttaaaaat ttaataaaat catactctaa gatgctttag tatttttatt ttatgataaa
     2401 aacttgttca gaccaaagtg g
//
LOCUS       DAEMAE010000341         2416 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEMAE010000341 DAEMAE010000000
VERSION     DAEMAE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.348"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            208..2220
                     /locus_tag="KPH93_003610"
     CDS             208..2220
                     /locus_tag="KPH93_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1690372.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNNKVKDILDISEDIYGKYIEDIFVDSLKVLLNEKEILDK
                     VVIFNRKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTTSPKMLECIDLAKKISKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYKWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSVNKNPSLKLKYNKNDILNILEILVLNKESDVGIGRGLILKTLLDKNLQ
                     ITEGKIKKIFEYLKKEELIICGSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1066 a          246 c          346 g          758 t
ORIGIN      
        1 aaatatacat acagtttaag ttttaagcaa caattagggg gatttggtga caagattttt
       61 aattataaga aagaaagtgt cttaatgcaa cttttagaaa gtcattatag acactttctt
      121 ttttatttga taaatttaaa ttatcaaaat tagattgctt tatacaacta ttttgttgga
      181 aactatttat tatataagga gaaataaatg aaaaaatcag tagctctggt aaatgatagt
      241 agaaaagatt taatagactt tttagaaaat aatttgaaat tagtttttgg agattctata
      301 aatattaata gatacttcat aaatgaaata aatgataatg atattataaa tgatgatgtg
      361 atattagtaa tgtcagtaga aagattggac aaaattataa ataatatact agataagaaa
      421 aaagtgattg tggtaagaag aacttttagg gaagataaaa tttataatct actatcttta
      481 ccacagggta caaatgctct aatcgtaaac gattctgatg aaactactct agagactata
      541 agtttatttt ataaaattgg agtgactaat attagaccaa ttccatatat gaatgataat
      601 aactataaaa atataaaaat agctataact ccaggagttc cagaaaaagt acctagcttt
      661 atatcagata tatttgattt aggtcacaga tatatagata tctctacatt tatagaaata
      721 ataaatttat tacaaattga ttcaaaggaa atacaatcta atctagttaa atactcagaa
      781 gaaataataa gtttagatac tggtataaaa gataaatata aggaattatt tttaaaaata
      841 gaagaattag atacaatatt aaatttatca aaagatggca tattatttac ttcaaaagat
      901 ggtgaaataa acacatacaa taataaggta aaagacattt tagacataag tgaggatata
      961 tatggaaaat atattgagga tatatttgta gatagcttaa aagttttatt gaatgaaaaa
     1021 gagatactag acaaagttgt catattcaat aggaaatata taaatgtaaa taaaaaaaac
     1081 atatacaata gagatgaaaa aatgggaaca tattatagtt tacaagaaat aacatacata
     1141 aaaaaattag aacaaaatct tactaaaaag ttaagagaga aaggacaaat agcaaaatat
     1201 acatttaaag atataaaaac aactagcccc aaaatgcttg agtgtataga tttagctaag
     1261 aagatttcta aatctgactt aagcatactt atcagaggcg aaagtggaac tggcaaggaa
     1321 ctcatagctc agtctataca taacaactca aatagaaaaa atcaaccctt tatagcagta
     1381 aattgtgcag ctgttcctga aaatttatta gaaagtcagc tattcggata tgacaaagga
     1441 acttttactg gaggtctaaa agatggaaaa caaggattat ttgaacttgc taataatgga
     1501 acaatattct tagatgaaat aggcgatatg cctttagaat tacaaacaaa actacttaga
     1561 gttcttcaag agaaacagat aatgcctgtt ggttctcaca atgtgattaa tatagatgtt
     1621 agaatcatat ctgctacaaa taagaattta gaacaaatga ttgataatag ccaatttaga
     1681 gaggatttat attatcgatt aaatacaatt ccaataaata tacctcctct aagagaaaga
     1741 aaagaagaca tattaattat tatggaagat ttaattaata agaaattagt aatcacacca
     1801 gaagcaaaaa aacttataca aaactataag tggaaaggta atataaggga acttcaaaat
     1861 gttacatcct atttaaacat tatgtgtgaa gatattgtat tagagaaaga tttacctcca
     1921 aacttaaggt cttcagttaa caaaaatcca tcattaaaat taaaatataa taagaatgat
     1981 atattgaata tattagagat tctagtttta aataaggaat cagatgttgg tattggtaga
     2041 ggattaattt taaaaacact actcgataag aaccttcaaa ttacagaagg aaaaattaaa
     2101 aaaatatttg aatatctaaa aaaagaagaa cttataattt gtggttcagg aagatatgga
     2161 agtaaaataa ctcaaaaagg agaagatttt tacaacaaat taaaatataa aggtctataa
     2221 atttataatc tattaaacaa agaaatctat tcaggagcta aatactcctt caataggttt
     2281 ttttattttt caagtacccc aaaattataa cttattttgt acataaataa taggaattaa
     2341 taggaatagg atattttttt atttcctatt tcttttatat aagctaagca tataagataa
     2401 attttctcca aaataa
//
LOCUS       DAEMAE010000342         2353 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEMAE010000342 DAEMAE010000000
VERSION     DAEMAE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.349"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            11..928
                     /locus_tag="KPH93_003611"
     CDS             11..928
                     /locus_tag="KPH93_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690373.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKMKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGAYLVGIDYSIPPK
                     DNLSGLNGTFERFSACNCDLIDISGQLYYPKPVPLTLGGLVTSIGTIPGVSELVALIE
                     AAVPATIDLGAILNAILDDIIDYIISTVNPLTNVDLASLCSLKAVAFNVTPADYEDFV
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVLVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1069..2250)
                     /locus_tag="KPH93_003612"
     CDS             complement(1069..2250)
                     /locus_tag="KPH93_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1690374.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRADLNE
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKVYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEVVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DVIVAATKTASELLKIDKNYGTLEKGKKADVIVVNGNPIEDIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          748 a          406 c          305 g          894 t
ORIGIN      
        1 agtgtattta atggaaaata ataaatgtag agaggacttt agatttactc aagaatacga
       61 ggaagattat ccaaatacaa atgaaagata ctatgaaaat tatcaagtag ctgatagata
      121 ctataattat ccaaataaat ataaagaacc taaaatgaaa caatgttgtt gtaaaaaaag
      181 tatgagagag gcattagaac ttctaagata tgatgctcta agaccttttg taaattttaa
      241 tcaatttgcc tttatttcag atttctttat cgttggtgct tatttagtag gtatagatta
      301 ttcaatccct ccaaaagata atttatctgg acttaatggt acttttgaaa gattttcagc
      361 ttgtaactgt gatttaatag atatatctgg tcaattgtat tatcctaagc ctgttccttt
      421 gacacttgga ggattagtta cttcaattgg tactattcca ggagtatctg aattagttgc
      481 tcttatagaa gcagcagttc ctgctactat agaccttgga gctatattaa atgcaatact
      541 tgatgatata attgattata taatttctac ggttaaccca ctaacaaatg tagatttagc
      601 atcattatgt agtcttaaag ctgttgcctt taatgttaca cctgcagatt atgaagattt
      661 tgtagcatct ttaggttact atcttgacaa aaaacattac aaagaatgta attgcaactg
      721 cgattgtgat gattgctgtt gtaataaagg tatcctagat aatctttata tgtcaaatat
      781 aaataatcaa gttactgtag tagctggtag tttggttcta actggtgttg aagttctagg
      841 taagaaaaat gatgttttag tacttggaaa ttctaatgat tcaagaatat actttgtatg
      901 tgtagattct atagattata ttgcatagaa tgattttaaa aagctatatc tgaatataga
      961 atagcattca ttttctaaag tataaaaaat ttaattatta ttaataaaaa agagtgccta
     1021 taattattaa aaatcccttt agacactctt ttttattttt tatgctactt atctaacttt
     1081 tttaccaagt ttaaatactg ctaatacatc tgcaagtaca tctatatcct ctattggatt
     1141 tccatttaca actataacat ctgctttctt tcctttttct aaagttccat aatttttatc
     1201 aatttttaaa agctcagatg ctgtttttgt agcagcaact attacatcca tattatccat
     1261 cccatagtct gacattagtt tcatttcata atatgttttt ccatgttcat tataagaagt
     1321 tcctgcatca gaaccaactg caattttaat tccttttttg tatgctttta ggaatgactc
     1381 cttgtgtgga atagcaacaa cttcacactt tctaagagta gcttcagtta ttccactttc
     1441 ttttcctgca tttagaataa aatatggtgc agccacagtt ggaactaagt aagttcctct
     1501 ttcaagcatc aaatcaatta tttcatcatc tagataaaca ccatgttcta tagaggtaat
     1561 tcctgcaagc actgcatttt ttataccagt tcttccttgt gcatgtgtgg cagttatttt
     1621 acctgctttt atagcttcat ctacagcagc cttcatttcc tccaaagtta attgtggtga
     1681 ccctggctca actccatcag ttgttacacc tccagttgcc attatcttta cacaatctac
     1741 tccatttttt aaaactgttc tggctgattt tctacattca tccactccat cactttcaat
     1801 accaaaatag tatccatgtc ctcctgtcat acatataact ggacctgaag taactatacc
     1861 aggaccatct atagaacctt tgtctataag atttttaagc tgtacatcca catatttggg
     1921 acaacctaag cttctaatat atgttactcc tgaatccaaa taagcatcaa gttgttttat
     1981 cgtatttact acaagctcta catctgatac attatcataa tatacctttg gatttccaac
     2041 tggctccatt gtcaagtgaa catgacaatt cataatgcct ggcataacag ttttatctaa
     2101 gtaatcgtac acatcatatt catttaaatc tgctcttata tcttcaattt caactatagt
     2161 atctccatca ataattatac ccttattttg cataatacta tttcctgttc catcaattaa
     2221 attccctgct ttaatatatt ttttgttcat cttaaatttc tccccctttt aagtacaatg
     2281 tattatttat ttattatttc taatattcta ataattaatt ttaccactat actcattcta
     2341 tttcaaattt aag
//
LOCUS       DAEMAE010000343         2333 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEMAE010000343 DAEMAE010000000
VERSION     DAEMAE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.350"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..353
                     /locus_tag="KPH93_003613"
     CDS             165..353
                     /locus_tag="KPH93_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG1690375.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(513..665)
                     /locus_tag="KPH93_003614"
     CDS             complement(513..665)
                     /locus_tag="KPH93_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG1690376.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(763..1238)
                     /locus_tag="KPH93_003615"
                     /pseudo
     CDS             complement(763..1238)
                     /locus_tag="KPH93_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            1457..2218
                     /locus_tag="KPH93_003616"
     CDS             1457..2218
                     /locus_tag="KPH93_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1690377.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLVKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIVASNKKLKNMSK
                     QDYEDWQNLSIEIISKLKKAFKTGDASSELAQEVARLHHKWLSYTWSTYSKEAHASLA
                     QMYVCDERFTSYYDKEQPGLTKFLRDAIVVYTRDS"
BASE COUNT          864 a          321 c          313 g          835 t
ORIGIN      
        1 atttacttta tataaaatta tatatataat tttatatgat aataagaaat tcttcaatat
       61 gtgtaaggca aatatatatt tctattgcaa atttttgtta tttatgataa tataaatatg
      121 tttttagtct ttaaatagtt atcattttaa ggaggtgtgc gtagatggca aaagtatgta
      181 gcgtatgtgg taaaggtaag gtttctggaa accaagtatc acactcaaat aaacataata
      241 aaagaacatg gtcagctaac ttaagaagcg taagagcgat tatagatgga gctcctaaga
      301 gagtaaaagt ttgtactaga tgtttacgtt ctggtaaaat tgaaagagct tagtaaccac
      361 gtttaaaagc ctacttccgt aagtaggctt tttaatttaa tcatttttac tctttctcat
      421 aaattttagg attatacttg aaatccattt tggtaatttt attgttacta ctttcatcaa
      481 tactcacccc acactttata gttaaaacta ttttaacaaa aaaataataa tgctccacat
      541 ccaataagta ttaatgcggt aagtgctgac caaatccagc caggaagcaa cattgacaga
      601 attacagcca ttcctgttgc tagacaaagt attcctagca ataccttaga cttgttattt
      661 ctcatggaac acctccacat agaacctgta tctataaata ttttcatatt caagtttatg
      721 cgacagtttt ccattttaga ccaataaaca tattatgtaa ttttaatttt tcatactaga
      781 attattactg aaatctgata tagattctac aatatccaaa attccttttt tcgtgcttaa
      841 cctttcaggt ccactatata aaaccacagg tataacttta ggaagtttaa agtttttatc
      901 ttctatttct tcaagacttt ttttcataat aaaactcttc caaatttgtt ctctatatga
      961 tttcaatctt gggacaattt cagtatccat ttctgtttga aactctaaca ctatataatg
     1021 tgatacttta ccacttttag aaaacacctt ataaattata tcaacttctt ttcctttata
     1081 ttcacaataa aagatgtttc taactctata tttttaaggg ttaattcttt tgctattgag
     1141 acacttgtaa aattttgaat caaactcaaa aaactttctt tatctgaata cattcttcta
     1201 tactcttctc gtttttcatt gcttcttttt aaattcatac agttcctcct agactaaaga
     1261 ctttctatat tttaataaag acagtctata aattttattt tttaattttc actagtgagt
     1321 attgacactt gtgtgtaaaa tatccagtaa agatattttt ttatattttt attaaaagat
     1381 ataaattttt atattttaat atattgacat ttacgttgcg taaaagtgta tatttaattt
     1441 ataggaggta ctacttatgg aatatacagt tcaaaaatta agtaaactag ctggtataag
     1501 tactagaaca cttagatatt atgatgaaat aggtcttctt aagccactta aaataaattc
     1561 ttcaggttat cgtatatatg gtcaaaatga agttaataaa cttcaacaaa tactttttta
     1621 tagagaatta ggaattaact tagaaaatat aaaaaacatt ataaattcac caaacttcga
     1681 tagtttaagt gcgttaaaag aacaccataa taaattacta gtcaaaagaa agcaaattga
     1741 tttactaata gaaaatgtaa ctaaaactat agctctgaag gagggaaaat acactatgac
     1801 agatatagaa aaatttgaag gttttaaaga aaagatgatt gatgaaaatg aaaaaaatta
     1861 tggaactgaa attagagaga agtatggtaa agatattata gttgcctcaa ataaaaaact
     1921 taaaaatatg tctaaacaag attatgaaga ttggcaaaat ctaagcattg aaataattag
     1981 taagcttaaa aaagctttta aaactggtga tgcctccagt gaacttgctc aagaagttgc
     2041 aagattacat cataagtggt tgagctacac ttggagcaca tattctaagg aagctcatgc
     2101 ctctttagca cagatgtatg tatgtgacga acgctttaca tcttattatg ataaagaaca
     2161 gcctggacta actaagtttc tgagggatgc tatagtagta tatacaagag atagttaata
     2221 taaaataata aagaggatgt ctcaaaatga actttttagt tcatgagaca ctctttttta
     2281 tatgaaatca aatatgtttt tatcatttca acaataaaaa aaggattatc tct
//
LOCUS       DAEMAE010000344         2333 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEMAE010000344 DAEMAE010000000
VERSION     DAEMAE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.351"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..1570
                     /locus_tag="KPH93_003617"
     CDS             119..1570
                     /locus_tag="KPH93_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG1690378.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKNGQAFRGGPAYYMEQGLNKKWMGVLFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMTMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLENVSEWHRELSEENLG"
     gene            complement(1792..2253)
                     /locus_tag="KPH93_003618"
     CDS             complement(1792..2253)
                     /locus_tag="KPH93_003618"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG1690379.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSS
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          792 a          265 c          398 g          878 t
ORIGIN      
        1 taatttgatt ttataaatat tataattaaa ttaatgaatt tttaacgagg aattattcat
       61 ttttttgatt ttaagattta ttgagaaaac aaaactaaat aggggggtaa ttaatataat
      121 ggattttttc aatggtattt taacgagtat taataatgca gtagtatttg taaacaatta
      181 cttatggtcg tatatcttaa ttataatgtt agttgtaatt ggtatttact ttactattaa
      241 aacaaatttt gtacaattca gatattttat agaaatgttt agacttctgg gtgatggtac
      301 agcgaataaa aatgcaaaaa aggaaggaaa aatctcttca ttccaagcat tttgtataag
      361 tacatcttca agagttggaa ctggaaatat agctggaata gcaattgcag tggtagcagg
      421 aggacctggt gctgtatttt ggatgtggct tatagcatta attggttctg catcaagctt
      481 tgttgagagt acattagctc agatttacaa agtgaaaaat ggtcaagctt ttagaggtgg
      541 accagcgtat tatatggaac aaggtctaaa taaaaaatgg atgggtgttt tattctcaat
      601 acttataact atatgttacg gttttgtatt taatgcagtt caagcaaata ctgtttcact
      661 tgcatttaat aatgcatttg gaataagtaa aatgactatg ggaataggtc ttggaatttt
      721 aactgcaata gttatttttg gtggagtgca tagagttgca aaagtttctg aaataatagt
      781 tcctgtattt gcaggattat atatattagt tgctttggct atagttgtaa tgaatataac
      841 agagatacca agtgttatag cattgatagt tgaaagtgca tttgatttta aaggaatggc
      901 tattggtaca tttatgggag ttgtaatgac tggagtgaag agaggtctat tttctaatga
      961 agcaggtatg ggtagtgctc ctaatgctgc tgcaacagca catgtaactc atcctgtaaa
     1021 acaaggttta attcaatctt taggtgtttt tacagatact ataataatat gtagttgtac
     1081 agcattcatg gtattattat attcaggtta tagtagtagt ggagcaactg gaattgaatt
     1141 agctcaagaa gctttaactc atcacatagg accagttgga aatatattta tagcagtttg
     1201 tatattcttg tttgcattta gttctatagt tggtaattac tattatggtg aatcaaatat
     1261 ggagtttatg agtggaagta agactaaact taatgtattt agagtttttg ttgtaggtat
     1321 ggttttattt ggttcactta ctcaagtaga tgtagtttgg aatcttgcag acttatttat
     1381 ggcattgatg gcgataataa acttaatagc aatagcactt ttaggaaaat atgcatttat
     1441 tgctttaaaa gattatacaa gtcaaaaaaa atcaggaata aaagaccctg tgtttattgc
     1501 agatgaaata gaaggtctgg aaaatgtatc agagtggcat agggaattat ctgaggaaaa
     1561 tttaggataa aaaaatagta taaaattaca aaatcatttt gaaagactag agtatgtata
     1621 tactttatat gaatataata ttgaattaat ttatattact ggaattttta attccagtaa
     1681 tattttttta ttatataatt tagttttatt aaaagttttt attattaaaa atagcttatt
     1741 tatcgtaaag taaatgatat tggcgttttt aattattatg aatgttggta tttaatttag
     1801 agacttaaat aaatttgcca tttctagtgc attcatagca caatcataac ctttatttcc
     1861 agcttttgta cctgctcttt caatagcttg ttcaatattt tctgtagtta cgattccaaa
     1921 tataacaggt aattcactgt ctaaagatac ttttgctatc cctttagaga cttcgctaca
     1981 tacataatca taatgagaag tagctcctct tataacacag cctaaacaga ttatagcatc
     2041 atacttacca cttttagcca ttttttgagc aacaagagga atctcaaaag ccccaggtac
     2101 ccaaactatt tctatatttt ctgaagaaac gtcgtgtctt aaaagacagt cttcagctcc
     2161 tgataaaagt tttgatgtta taaattcatt aaaacgagaa tttattatac ctatttttaa
     2221 atcttttcca ataagttttc cttcatatat cattttattt cctccaattt tatttatgtt
     2281 atagatttat attttaaata tttttaattt taattataaa aaactactta gta
//
LOCUS       DAEMAE010000345         2327 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEMAE010000345 DAEMAE010000000
VERSION     DAEMAE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.352"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            294..2312
                     /locus_tag="KPH93_003619"
     CDS             294..2312
                     /locus_tag="KPH93_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG1690380.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMISIPVGKSEFPMRLLALAAGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKVDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERINLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEVNYIKLCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPREFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLK
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RNVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIEEAA"
BASE COUNT          974 a          188 c          369 g          796 t
ORIGIN      
        1 tgggtaaaga aaagcttaat taaatttatt gtataaatta taactctctt aataaataca
       61 tttttagtat tgttgagaga gttattttta tatttttgaa aaaatatttg aaagaaaaaa
      121 tatatgggag catacgaaaa tatataaact atatgtaaaa aaaatacgtt taaatatgaa
      181 ttatatagct ctgaacataa aaaaagttaa agtcctaccg taaaagagtg tattttgaat
      241 aagataacaa atatgataaa attggtcttg ttgaaatttt taggggggat atgatgacaa
      301 agaaactagt aatatcaaaa acaaagttgt ttatttttat aatagcattt atttctatat
      361 ttatatattt atttggaagt aaaaacacac ttataggtgt aggtatagta acagctatgc
      421 taacactcct ggaaagagat ttaactattt ctccaattaa aaatttacta aagtatttag
      481 caataaatat aatcttagga atactatcat tttttgcagt acaaaatatg tatttaggcg
      541 ttttattaaa ttttatagca ttatttataa tagggtatgt atttagttat gacttaaaaa
      601 gagcagtata tgtacctttt ggtctaatgt atatatttat gataagtata cctgtaggaa
      661 aaagtgaatt tcctatgaga ttgttggctt tagctgctgg agcagttata ataatgattg
      721 ctcaatttgt catgaataga aataggatga aaaatgtcgg agataaagaa ctgatatcta
      781 tatgtgatga attattagaa aaaattagtc ttttaaaaaa tactattaat gatgatagtt
      841 ctataattaa atctagtatg agaaaagtag atagctgtaa ttatagaata aattctatat
      901 ctaaaaattt gaaaatggtt atttttgata atagaaaaga tgacttttat attagtatta
      961 gaggcataga cataatgaat attttatttt cattagaaag aataaattta atcctggaaa
     1021 gatacaaaaa ggatagtaaa gaatttgaag atgaagatat aaaaaatatt ttagaagaaa
     1081 gtagcataaa tagtaaatca gatgttctag ttgtagcaac aaaagaagta aactatatta
     1141 agttgtgttt agaaaataaa gataccataa ctgataaaga gatattagga tttagagatt
     1201 atgttattgc tcaggatact aaaaatataa atttaaaaga aatttatagt gttttagaaa
     1261 atttatatga gtttttagta gattataaaa aagtaaaatc aagagacgaa aagaaagctg
     1321 aaagaaaatc aaagatacct cgtgaattta aacgactatc aatatataag aaaaacttta
     1381 atttaaattc tataagattt tcttatgcag taaagattgc tttagctaca gctgtagcgg
     1441 gttttattat ggattatttc catttaaagg atggaagatg gataatgtta actgtctttt
     1501 cattaacaca accttatgca gaaaattgta tacaaaggtc tagaaaaaga atagaaggta
     1561 catttatagg agctgtaata tttattgtat tattttcaat aataaaagat agtacattaa
     1621 ggtcgttaat agttctttta gcagggtata taaactctta tgtagttgat tatagaaaat
     1681 taatggtatg tgtaactgtt tcagctcttg gttcagcagt agttatggga gacccaaatg
     1741 ttttaacaat aagcaggatt tcatatgtag cattaggagc aattattgca ttaatcgtaa
     1801 ataagtttat attaccatat gatgctaaaa caggatatca acatgttata gaaatgtata
     1861 aaggaatagt taaaaatata attgatgaag tcaataagtc tatagaaaat gtagctgatg
     1921 tttactatat aaaaaattta ttattgatac catcacttat tgaagataga cttatgttga
     1981 taaatgctat atataaagat aaacatcaag aggatttttt agaaaatcag aaattactaa
     2041 taagtaacat gtataatcta tatataaatg ttaataaaaa taaaataaaa gatgaagatg
     2101 ttgaaaaaat attgagagat actaattata tttcaaatta caatgtagac aagtatgatg
     2161 aaggaagaaa tgtgatttta gaaagtattg taaatactaa aagtctaggt gacaagataa
     2221 tatgtctaaa cttactgcaa actctaaatg gtgtcaaaga aatgtataga ataagtaata
     2281 taactaaagt ttcaatagaa gaagcagctt agatattaaa ttataga
//
LOCUS       DAEMAE010000346         2261 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEMAE010000346 DAEMAE010000000
VERSION     DAEMAE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.353"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..434
                     /gene="trxA"
                     /locus_tag="KPH93_003620"
     CDS             117..434
                     /gene="trxA"
                     /locus_tag="KPH93_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG1690381.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(640..1914)
                     /locus_tag="KPH93_003621"
     CDS             complement(640..1914)
                     /locus_tag="KPH93_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG1690382.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCDIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARMAHKYGAKILVDIVQVIQHRPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGSKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYIRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIYAIKTLAFK"
BASE COUNT          796 a          307 c          321 g          837 t
ORIGIN      
        1 atttatcata atattaaatc tattaaaaat aggcggttac aaaaaagtgc ctaattgaac
       61 tgttatatag gtcgtagtat attaatatca agaagacaat aaaggagatg tatattatgg
      121 taaaaataat aaataataat gaatttataa atgaagtaga aaataaagat ggtttagtag
      181 ttgtagattt ctttgcgact tggtgtggac cttgtaagat gctatcacca atatatgaag
      241 ctttaggaaa tgagatggtt gaaaaagcaa atttcttgaa ggtggatata gaccaaagta
      301 tagaattagc tcagaaattt gaagtatcta cagtaccaac tatgctaata tttaaggatg
      361 gaaaacctgt agatagatta ataggtttta tgcctaagga gaatttaaaa aataaaattg
      421 aatcatatat gtaattaaat taaattgcag taattaaaat aaatctcata tataaacaag
      481 aaactcattg taatgagttt tttgtttata cagtgtaaaa tagtttgtat agttacaaat
      541 gagtgcctaa ttgctaatga gattgactat tgatataatt tgcatttttg tgaaaacaaa
      601 actattctct atttattgct aaaaaagaat agaaaaatct tatttaaaag ccaaagtttt
      661 aatagcatat ataaagctat caacctcatc aaaagtattg tacattccta agcttatcct
      721 aacaagacct acaggatttt ccctcataaa tctgatgtat gcctcttttg gacttatacc
      781 taacaatttt tgtacataaa tatctgcacc tactactcca gctccagttt ctattccaaa
      841 gttatcaact aaatattttg ctacatcttt gtaatacata ttacttatat taaaagatat
      901 atttgggata attacattat ttgatttatc tccatatatt attatattag gtattttact
      961 catttcttct ttagcatata aaaataattc tctttcgtat tttgaaattc tgtttagacc
     1021 aattttattt aaagtgttca gtgcttttcc cattgcaaga gttccaaaat actctggata
     1081 tcctgcttca aaacgttggg gcacgtttgc atatagtatt ttgttatcat ttacaaattt
     1141 ggtagaacct gagccatata gtacaggatt aaatttttca aagaattttt tagatcctac
     1201 cagagctcct ccatttagtg gagaataaca tttatgagca ctaaaaacaa caaaatctat
     1261 atgctcatca cattcatgtg gcatcattga aaaaggtcta tgctgaatta cttgcacaat
     1321 atccaccaat atctttgcac catacttatg agccattctt gctatttcat atataggagt
     1381 ggttatacct gtaacattac tagctcctgt aactgtaacg agagatacat taccagcata
     1441 atttttaagt atattctcta attgacataa atcaagttct cctttttctg taattccaac
     1501 aacttttgta ggaaaattaa ctttaaaagg caggtaatta gccatatgct ccattgctgt
     1561 ggtaataaca aatttatctg gattttcatc tttaaaaaat tttgctaaga agtttattcc
     1621 ttctgtggca tttttgacat aaataacaga atccttttct tcgtctccat caataagttt
     1681 gaggatagta gagcgtacat tttcatattt taaagtattt ttttctgaga ttattcctgg
     1741 agcattaata taagtaagcc aaggaatatc acagtttaag ttatccacta cgttttttaa
     1801 aactaatgga gtggcagagt tgttaaaata tattctttta acagtttcac catttttagt
     1861 tttaacttta aggtttgcac cttcaatgta tttagataca tccatgaatt gcataaaaaa
     1921 aaccccttat ctaataaact attctgtttt ttattaaaaa agctcaagtt ttaatcaaga
     1981 gcttgtttaa tatatgttat gtttttataa ggggttttgt taattaattc ttgttgagat
     2041 gtttattaaa ttacacgaaa atgagctaca gttgggggaa actgtagctc acttgatttt
     2101 gggggagata ggttgaactt attgtttatc cgtatcatat ttttagtata gtcacttatt
     2161 ttaatttata tacatttttt tataaatttt acataaaatg ttaaacgcta attagaaata
     2221 attatgacta tgactattaa agttgcaaaa atagtaaaga a
//
LOCUS       DAEMAE010000347         2249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEMAE010000347 DAEMAE010000000
VERSION     DAEMAE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.354"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            101..2173
                     /locus_tag="KPH93_003622"
     CDS             101..2173
                     /locus_tag="KPH93_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1690383.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMIDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIITSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFLEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAVMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNVEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RDGIEKIG"
BASE COUNT          824 a          248 c          439 g          738 t
ORIGIN      
        1 cataagttta atcttagaat taagtatact aatgtaactt tttataaact taagtctttt
       61 ttatttaaaa aaatataaaa taataaggag agaaaagtaa atgaaaaaag tatttggtgt
      121 tttacaaaaa gtaggaaaat ctctaatgct accagttgca ttacttcctg cagcaggtat
      181 attacttggc gtgagtaatg ctttagcagg agatgcattg ttggctcatt ttccagcttt
      241 ggcgaatcct acatttcaac ttataataag tataatggaa agttcaggtc aaattatatt
      301 taataactta ccacttatat ttgcagttgg ggtagctgtt ggtttaactg aaggagaagg
      361 tgttgcagca ttggcagcca tagtaggttt tttaattctc aatacatcta tgggtgtcat
      421 ggcaggtgtt acagaagata tgatagataa ttctatgtat gcaagtattg ttggaatacc
      481 aacaattcaa acaggggtat ttggtggtat tattataggg atagtggctg cttatcttta
      541 taaaaagttt tataaaattg aattacctca gtatctaggt ttttttgcag gtaaaagatt
      601 tgtaccaatc attacttcat tagttgctat agcaatagga attatattgt cttttatttg
      661 gcctccaata caagagggat tacttagttt ctcaaaaaat gttatagaag caaatcaaac
      721 actcgcagca tttttggaag gttctttgga aagactatta ataccatttg gtttgcatca
      781 tgttttccat aatccatttt ggtatcaatt tggagaatat gtaaataaag ctggtgagct
      841 tgtaatgggt gaccaaaaga tattctttgc tcaacttaaa gatggagtaa atttcacagc
      901 aggtactttt atgacaggtg catttccatt tatgatgttt ggtcttccag cagcagctct
      961 tgctatggtg catgaggcaa agccagaaaa gaaaaaattt gtagcaggta taatggcatc
     1021 agcagcatta acatcttttt taacaggaat aaccgagcca atagagtttg cgtttttatt
     1081 tgttgcacca gtattatttg caatacattg tttattttca ggtttatcat acgcagttat
     1141 gcaattatta ggagttaaaa ttggattcac attttcagga ggattaatag atttcttatt
     1201 atttggtgtt cttcctaata gaactccatg gtacttggct attttggtag gtattggttt
     1261 tgcagtatta tactatagcg tatttagatt tatgataaga aaatttaatt taaagacacc
     1321 aggaagagaa gatgagtcag aaactaatgt tgaagtcatt gaaatgtcta agaatgagtt
     1381 agcatcagag attttgattg ctttaggaaa taaagacaac ttaaagagtc tagatgcatg
     1441 tataacaaga cttagattgg ttgtaaacga tataaataaa gtagataaag aaaaattgaa
     1501 atcacttgga gcagcaggtg ttatggttat tggcaacaat gttcaagcta tatttggacc
     1561 aaaatcggat tcaataaagt ctgatataaa agatataata gagggaaaaa caaaagttga
     1621 aagtactgtt actaaaaatg ttgataaaaa taaagtgaaa aaaaatatgg aagttgccaa
     1681 tatggaaaat gctttagtag aaaagattga ttcattaaca aatggagaga tattagatat
     1741 agaggaagta ccagattctg tattttcaag taaactgatg ggggatggat ttgcaattaa
     1801 atctagtgat ggattgattt attcaccagt tgatggaact attggagtta tatttccaac
     1861 taagcatgca ataataataa aatccaaaaa aagtggagta gaaatactta tacatttagg
     1921 gatagaaact gtaaatttag aaggaaatgg atttgaagta tttgtgaatg taggagatga
     1981 agtaaaagca ggagaaaaat tagttaaaat ggacttagaa tatattgaaa aaaatggtct
     2041 atcaacaata agtcctgtgg tatttacgaa tttagaacaa aatcagaaat taagcattaa
     2101 aaaaggaata actacagcaa aagaagagaa tagagtaagt atattaagag atggtattga
     2161 aaaaatagga tagctatatg atagtataac taaaagttat gtgagattta ataaatatat
     2221 aaataggttc agttaacatg caatttatt
//
LOCUS       DAEMAE010000348         2248 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEMAE010000348 DAEMAE010000000
VERSION     DAEMAE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.355"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..1447
                     /locus_tag="KPH93_003623"
     CDS             134..1447
                     /locus_tag="KPH93_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG1690384.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GSYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRASESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTHFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1465..2001
                     /locus_tag="KPH93_003624"
     CDS             1465..2001
                     /locus_tag="KPH93_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG1690385.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          890 a          212 c          411 g          735 t
ORIGIN      
        1 attataatta tatgtattta gttggatttg tgtatttaaa taatgtataa tttatgctga
       61 ttactgctaa aaaagcataa taatattgta ttgtaaggtt ttataaactt ctaattgaaa
      121 ggggataatt caaatggata agagatattt agaatccgat gtatttgaag cttttcaaga
      181 aagactttct tatcttttta atgaatttga ttatatctat gtatctttta gtggaggaaa
      241 agatagtggc ttactgctaa atttagtttt ggattataag agaaagaata atataagttc
      301 caaaatagga gtatttcatc aagattttga ggctcaatat agtttgacta ctgaatatgt
      361 tgaaaaagta tttgaagaga acattgaaga tattgaacca tattgggtgt gtttaccaat
      421 ggctacaaga actgctgtag gaagttatga aatgtattgg tatccatggg atgatgagaa
      481 agaagaatta tgggtaagag aaatgccagt caaagaatac ataataaatc aaaaaaataa
      541 tccttttact tattataaat ataaaatgag acaggaaagt ttggcaaaac aatttggtag
      601 atggattaga gatatacatg aaggtgcaaa agttgtttgc ttattaggaa cacgagcatc
      661 agaatctcta caacgttatt gttctattgt gaataaacag catggatata aaggaaagtg
      721 ctggataact aaggaattta aaaatgtttg gacggcatct cctatatatg attgggaaat
      781 agaagatata tggctagccc actataaatt tgattatgac tataataaaa tttatgactt
      841 attctataaa gctggagtag ctccaagaaa aatgagagta gcatcacctt ttaatgattc
      901 agcaaaagag agtttaaatt tatacagaat attggaccca gatgtatggg tgaaattggt
      961 tgctagagta caaggagtta attttacttc tatttatgga aaaacaaagg ctatgggata
     1021 tcgtagtgtt aaattgccct atggacacac atggaaaagt tttacacatt ttttgcttga
     1081 taccttacct gaaaatataa ggaatagcta tattaaaaaa ttttcaacat ctattgagtt
     1141 ttggcagaat acaggtggtg gtttgtcaga aatagttata cgtgaattgt tgcgaaaagg
     1201 ttataaaata gaattaaatg gagtttcaaa tcatacaaaa gataaaaaga gtagagtcat
     1261 atttttggat gatatacctg atgatacaga tgatattaaa tttaccaaag atataccaag
     1321 ttggaaaaga atgtgttatt gtattttaaa aaatgaccat atgtgtagat ttatgggatt
     1381 tggtcctaca aaagaacaga gaattagaac ggaatatatt aagaagaaat atgaaaaaat
     1441 tcattagata ggagttaaga tgctatggaa tataaaagtc ctgtatatgg aatcatcgca
     1501 gtgccaatcg aaaagataga atctaacgac tataatccaa atgcagtagc cccaccagaa
     1561 atgcagttgt tgtataaaag cataaaagaa gatgggtata caatgccaat tgtctgttat
     1621 tataatgagg aaaaggataa gtatattatt gtagatggtt ttcatcgata tagagtaatg
     1681 cttgaaaatg aggatatata tgatagagaa aatggacttc ttcctgtttc agtaattgat
     1741 aaatctcttg atgaaagaat ggcaagtact attcgacaca atcgtgcaag aggttctcat
     1801 aatgtggatt taatgagtaa aattataact gaactttgtg aaattggaaa atctgatgaa
     1861 tggatatcaa agcacctggg tatggaagtt gatgaaattc ttagattgaa gcaaataact
     1921 ggattagctt atttatttaa agataaagag tttggtaatg catggattcc ttttggctct
     1981 gatatagaag aagaaaatta aatagattta ttaaaagaaa aatatatatt aaaaaagaaa
     2041 atttaaaata tatattaaaa aataaagagt tttaatatta tatataagca cagaaaaaag
     2101 agtaggaaaa atatcctatt ctttttttat ttttataatt atactcttag aataaataaa
     2161 attatataac taaatacatt ttaatataga attatttata aaaaaatagt attaaattgt
     2221 atatttatat cgtttttaat aaaaaata
//
LOCUS       DAEMAE010000349         2230 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEMAE010000349 DAEMAE010000000
VERSION     DAEMAE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.356"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            789..2144
                     /locus_tag="KPH93_003625"
     CDS             789..2144
                     /locus_tag="KPH93_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG1690386.1"
                     /translation="MSSTVKKKGKFPIGFYICSTTFSFERAAYYSSKYLIYIFLTTAI
                     IHGGLGIEKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDNQEELDTAFSVQYSFVNIGSFI
                     GTTAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFTYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMTVG
                     GFEVPLAWFDSLNALVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASIIWIIMFGILLSFGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGQGYGYIYAFTLKFEMIKVYTIIPVILFVSAILLFLFDKTLSRLVEDD
                     K"
BASE COUNT          777 a          248 c          392 g          813 t
ORIGIN      
        1 cttgacaaat ataatttaaa tttttctaat tatttactgt cgattaatag atttttttct
       61 tgcatataag tatgtattta tggtacgatt tacatggtga aataaaaaag gggaggtgaa
      121 aaataagtat tcgtgaaaaa gttttcagtt tgaggtattt tataaataat atattataaa
      181 taaagagtat aacctcaata aatagtacca aagcgaatat atgggtgatt actttaaaat
      241 aacagttttt atgtttagat tcacagtgtt tattataaac taatgtgtaa gaaataagtc
      301 taatctgtga atagatagta gtttatttat ttaagttgag aattggttgg gtgaagtaat
      361 atataaattt aaaaagtaaa taaaaacatt atttgaacaa atatatgttg ctttattcga
      421 gactccttta aaaaagcact aaaatgtaat tacagaagtt gctacaaaag gggaatttta
      481 gtttgtaaaa tactatcacg taaaaaataa gaattgcata tggcgaaggt tttttcatgg
      541 tattagatga gaatatactg atttataaat atatctgaaa atagtgaatt tgtataattt
      601 aaatttatga ctaagaagtg tattaatttt attccatgaa aatatgtact ctacaaataa
      661 atgcgcaatt atcttaataa taattacaaa ttgttttaca aaataatatc ataatatctt
      721 tcataaaaag aaagaatttg gacaaaacgt aaaaaaataa aaatacaatt aagaaaggat
      781 ttaaaactat gagctcaaca gtaaaaaaga agggtaaatt tcctatagga ttttatatct
      841 gctcaacaac tttctcattt gaaagagcag catattattc ttcaaagtac ttaatctata
      901 tattccttac aacagctata attcatggtg gtcttggaat agaaaaggga caagctgcta
      961 taatgcaagc taatcttgtt gcattcacat atcttgcacc tataattggg ggatatatat
     1021 cagatagatg gataggagct agatatacta tacctatagg tatgcttatc atgggggtag
     1081 gatattatct tggttctatt gcaactacag tttctatggt aaatgcaatg ataatattag
     1141 tttcaattgg tactgcattc tttaaaggga atgtatctgc agtaaatgga caactctttg
     1201 ataatcaaga agaacttgat acagcatttt cagtacagta ttcttttgtt aacataggtt
     1261 cttttattgg aactacagca gttggtattc tttaccttaa aacatttgca aaaaatggtg
     1321 tacttggatt ttctcaatgt ttctttatag cagctgtact ttgtgtaata ggtgcaatat
     1381 ggttcacata tggatggaga ttccttggaa atgcaggaaa gagacctttt aaagaaggcg
     1441 tagttgctga aaaagttgaa gaaaaagata agtctcctct tacaagtatg gataagaaga
     1501 gaatttgggc tattatactt atatcattct tctctgtaat tttctgggta ttctggtatc
     1561 taacttatct tgcagtttat gattatggtg cagcatttgt aaacatgact gttggaggat
     1621 ttgaagttcc tcttgcttgg tttgactcat taaatgcact tgtatgtata gtattaggac
     1681 cagttcttgg agcattatgg tttaaacttg ctagtagacc acaaggagat atgagtttat
     1741 ttaaaaagac aggtcttgga ttaatattct taggtcttgc attcttaatg cttgttggtg
     1801 cagaattttc aagaggggtt ggagctcctg aaactgctaa agctagtata atatggataa
     1861 tcatgtttgg tattctttta agttttggtg aaatgttatt ctcaccactt ggtaactctt
     1921 tcgtaagtaa gtatgcacct aagaagcttc taggtgttct tatgggagta tggacatttg
     1981 ctacttttat agctggtcaa ggatacggat atatatatgc atttactctt aaatttgaga
     2041 tgattaaagt gtatactata attccagtga tactatttgt ttcagctata ttattattct
     2101 tatttgacaa gacattaagt agacttgttg aggatgataa ataatcagta agttaattat
     2161 tgtgttttat agattaatct attgtaatta tgttgtatta gattgattta taactaatta
     2221 ataataagaa
//
LOCUS       DAEMAE010000350         2215 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEMAE010000350 DAEMAE010000000
VERSION     DAEMAE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.357"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(18..647)
                     /locus_tag="KPH93_003626"
     CDS             complement(18..647)
                     /locus_tag="KPH93_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG1690387.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHETILEKAKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVVPNYSGEKYYKMESVVNDENLV
                     TAPGTAPLEFTFQVLKILDVFESESLNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(726..1688)
                     /locus_tag="KPH93_003627"
     CDS             complement(726..1688)
                     /locus_tag="KPH93_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG1690388.1"
                     /translation="MAKIDNMMSILWMLSSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKALLHAALFAKKEGYFLDEALDKA
                     ILKLKMYSNQKQEDILTNHLIALEVIKPIGKLSIESVLKKLENSILKELSVEINYHTG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEIRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKNEQGIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIQPLSLRKKMIEDLLELIDYYQI"
BASE COUNT          727 a          351 c          230 g          907 t
ORIGIN      
        1 aaaaataaat cttaaattta tttattacaa ttcattaatt caaaaaaata ttttgagtcg
       61 tgagttttgt aaagatttaa ccatgaattt aatgattctg attcaaaaac atctaaaatt
      121 ttcaatactt gaaatgtaaa ttccaacgga gcagttcctg gagctgtaac taaattttca
      181 tcattgacta cagattccat tttataatat ttttctccag aataatttgg tactaccatt
      241 ttaagaaact ctaagtcatt acttgtatgg tttctaaaat ctaatagccc ttttttagct
      301 agacctaatg tagctccaca tatggctgca actatagaat cttgtattaa agcttcttta
      361 gctttttcta atattgtctc atgattcata gataaccaag tgtttccccc tggcaaaatt
      421 aataaatcct tgctttccat attaaactca tcaactgtaa tttcaggtaa aacttttaac
      481 ccacccattg ttataatagg atttttatca attcctacag taattacttt tagtggtttt
      541 aatccttttt taaaatatct tcctgtattt aattcagcag ttaaatatgc cacttcccaa
      601 tctgacatag tatcaaatac atacatatat acttttctat ttttcatctt ttttactcct
      661 tatgtaatta atttatatat ctaattgtaa aataacttaa ctgacatatt cagtcagtta
      721 agttattaaa tttgataata atcaattaat tctagtaaat cttcaatcat tttcttccta
      781 aggcttagtg gttgaattac ttgtattgtc tttctataag gtaaaagtat atttggtatg
      841 tacttgcata ttacttcctc ttcaagcata aatattattt tattaggtgt tctttctttt
      901 aagtagtgcc ctaaaaacca atgttggctt aaactatcta aagaatctat actcccttct
      961 acaactaatt gaattatacc ttgttcattt tttatttcag gtataatccc ttttataaaa
     1021 aattcacttg ccgaaaattc tgtcggtctg ttaaatgtat ttgatgtttc tatgatttca
     1081 ctaatcctat ccactctgaa actacgaatc tcatttctca tattacaaaa ggcaactacg
     1141 taccaattat tattccaatg aacaattcca tatggattta taattctata ttttgatttt
     1201 gctttacgac ctgtatgata gttgatttct acagatagct cctttaatat actattctcc
     1261 aattttttaa gtactgattc tatagataat ttcccaattg gttttattac ttcaagagct
     1321 attaaatggt ttgttaaaat atcttcttgt ttttgatttg aatacatttt taactttaat
     1381 atcgctttat ctaatgcttc gtctaagaag tatccttctt ttttagcaaa tagagctgca
     1441 tgaagtagag ctttttgctc ttctatatca aaaaaaagag gtgctttagt aaaattattt
     1501 aaaaggctat acccaccatt atgacctgta tctgaaatta taggtactcc actcgcagaa
     1561 agtacatcaa tatatcggta cactgttctt atatttattt ctaatttttc agctatctgt
     1621 tttgctgtaa tttttttgtc agagcttaac atccacagta tagacatcat attgtcaatt
     1681 ttagccatgt agtttcacct ctttctattt gttatctaaa gctattatat aactttttga
     1741 ttgattttta caaaaaaata ctatcaagct gctaataatt tctactaaga tttatttcaa
     1801 atatttacaa atagtctttt tctatagtta agttatctct taaattacat agtagtatat
     1861 ctattttagt tgatatattt tcagctacct cccttgaata ttattacaat aagtctaaat
     1921 cttctttttt ccataaatat attatgagga aagactatca tctttaatgg tttaatagta
     1981 taaaagatat taatttaaaa aactctattt ttatatcaga tgggtattgt tttttataaa
     2041 actgaaaaaa tcacctaata gaatctatga ttttttaaca taacctccat aaattagtac
     2101 caattatcac ttcaaattca aatttttatc taaagaatat acaatcatat ttccatactc
     2161 aacataatat tgagtgtttg gcttgtattt acaaattatt attctaacaa aaaca
//
LOCUS       DAEMAE010000351         2187 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEMAE010000351 DAEMAE010000000
VERSION     DAEMAE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.358"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            306..920
                     /locus_tag="KPH93_003628"
     CDS             306..920
                     /locus_tag="KPH93_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG1690389.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYSSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDIAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            938..1300
                     /locus_tag="KPH93_003629"
     CDS             938..1300
                     /locus_tag="KPH93_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690390.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIN
                     FMLDNGMEDILKEKGVLK"
     gene            1406..1903
                     /locus_tag="KPH93_003630"
     CDS             1406..1903
                     /locus_tag="KPH93_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG1690391.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELKENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHIYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          848 a          234 c          341 g          764 t
ORIGIN      
        1 gattggtaaa atattcatat catataagta ttactatata ataatttatt ctattagtat
       61 actcaaatca tgtaaaattt atataactca attaattttt taaatacaac tttataatat
      121 aaaacttata ataaaagtaa atttatatgt taaaggaggt tgatttttta ttatttttta
      181 tttatgctag tactaaagac ttgcaaacgt tttttgacaa gtctttattt tgtctaaatt
      241 aaacaaactt ttttacaaat aaatacataa aatttaaact aatctactta ttggaggaga
      301 taaaattgga agaagtaatt gcaaaaatta ttactataga aaatggattc aaagaaattg
      361 aaaacattgc taaaaaaatt gtaaaaaata atgactcaaa aaagtgttat tatctatcaa
      421 tctacttata taggtcagaa tattatcaag taagagaatt ggctgtgttt atacttggtt
      481 ttatatcagt aactatttct gaagctttgt tatttttaaa agacaatgtg agtaaagatg
      541 aaaactggag agtacaagaa atattagcta aatcatttga ttattattcc agtgaagtag
      601 gatatgaaaa ggcacttcct gttataaaag aatggcttaa ttctgatagt ccaaatataa
      661 aaagagctgt gacagaaggt cttagaattt ggactagtag agactatttt aaatctaatc
      721 ctgacattgc tatatcacta ctttctagtt taaaagaaga tgatagcgaa tacttgagaa
      781 agtctgtagg taatgctctt agagatataa gtaaaaaata tccagattta gtaaaaaaag
      841 aattagattc atgggatata tcaaataaaa aagttgagtt tgtatataag cttgcttcaa
      901 aatgtatctt ggcaaagtaa aatggaggtg aacttatttg aaacttcgtg atgagtatac
      961 ttgcccatta gaactaacac atgatatgat aaaaggcaaa tggaaatcta ttatattatg
     1021 gagattacgc ataggaccaa cttccttatc aaaacttgaa aaggaaatca atggtattaa
     1081 tcaaaagatg ttacttgaac aacttaaaga attaattgaa tttggtcttg tagataaaaa
     1141 gacatatgaa ggttatccac ttaaagttga atactcactt acacctattc gagggaaaca
     1201 aatattggat gctttggcta taatgcaaga tataggaatt aactttatgt tggataatgg
     1261 tatggaagat attcttaagg aaaaaggtgt tttaaagtaa atagataccc acaaaaaagt
     1321 aagtaattta ccattatttt caactggtat ataattaaat tatagagata ttctattaat
     1381 ttattataat aggaggtaaa ttattatgaa agtaactagt acaggtatag taaatggtgt
     1441 tatagaagat aaatacggta aaagaggaac acagttcaat gaaggtggta tgcctacata
     1501 ttcactacct ctaaagtttg aagatgctcc aaagaataca gtatcctttg ctgtgttttt
     1561 agaagataaa gactctgttc ctgtatgtgg atttgcatgg atacattggt tggctgtaaa
     1621 tataacaaaa gatgagttaa aggaaaatga aagtgtaaca gctactgatt ttgtacaggg
     1681 gactactagt tggcatggaa aactaggagg tcttgacaga ttagaggcca gtgtatatgg
     1741 cggaatggct cctccagaca gacctcatat ctatgaaata catgtatttg cacttgatac
     1801 tatccttgac cttgaaaaag gattctatat gaatgaattt ttcaaagcta ttgaagggca
     1861 tgttttagat acatttacac taaaaggaag ttattctaat taattaggtt gactatagct
     1921 aatcattaat taagtgccta catttaattg atgattagct taattattat taaaatattt
     1981 tttgtatttt attttaatat atattttagt tgactgatat tttaatttat attagattat
     2041 tatcaattta tataagttga ttttagctta aaacttactt atatatttca atttctatta
     2101 gaattattat taaactcatt aaaataaaaa ccttaatata actaataata tatgttaaac
     2161 tcattaactc tacataaagt tttataa
//
LOCUS       DAEMAE010000352         2184 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEMAE010000352 DAEMAE010000000
VERSION     DAEMAE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.359"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..979)
                     /locus_tag="KPH93_003631"
     CDS             complement(<1..979)
                     /locus_tag="KPH93_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1690392.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLADLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVA"
     gene            complement(1136..1948)
                     /locus_tag="KPH93_003632"
     CDS             complement(1136..1948)
                     /locus_tag="KPH93_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1690393.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKFLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKIYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGGACEKISSITKEHYTMIKGNDRFDTLCKALDFINR"
     gene            complement(2027..>2184)
                     /locus_tag="KPH93_003633"
     CDS             complement(2027..>2184)
                     /locus_tag="KPH93_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection bacteriophage resistance
                     protein"
                     /protein_id="HBG1690394.1"
                     /translation="SYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIGFPNN
                     WIEILAK"
BASE COUNT          713 a          366 c          189 g          916 t
ORIGIN      
        1 ttgctacaca ctctagatat tttaaattct tattgctact tatatccaaa ttcttcagat
       61 tacttctact acaagttaaa tcttccaaat taatacaatt ttccacattt aaagtactta
      121 attgttcttg attacaatat agttttacaa gtttttcatg attttttaaa tttaaatttt
      181 gcaatttatt gttgtgacaa attaacttaa caagttcttt attattatct aaatttatat
      241 tttctaattg attaaaagaa cagtttaaat cctgtaattc taaatttgta gatatatcta
      301 aatcatttaa attattataa tcacatttca aaatttttaa tttctgattc ttagataagt
      361 tcaaactagt caattcattt tcaaaacata aaagagttgt caaattagtt gctttagtta
      421 tatctaaact ctttaaatta ttttttctac agttcaattc gtctaattta gaattttcta
      481 taactaagtc tgctagatta ttttctccac aatgcaacgt tgttaactct ctattattac
      541 ttaaatctag gcttgttaat tgattttcgt tacaatacaa ttcttttaaa tttatgttgc
      601 tacttaaatc tagactcgtt aaattatttc ctctacaact aaaattttct aatccaataa
      661 aataattaat tccagataaa ttttttattg atttgctccc taaggttaaa ccttttgtag
      721 attctataat cgctttttgt gaaattccaa ttggatttcc atcatctaaa atacttagtt
      781 tatcacaaat tacttttcta aaattttcat ctggaaaagc ttcttccatt gtcatatttt
      841 ctaagttatt agctttcctt gtttcatatc taacttccct atttttctca atatcttgtg
      901 catttatctt atgtaccaaa ggtgatacaa ctaccaatga aagtgataaa ataactgctt
      961 ttttgcttaa atttttcatc cccacacctc attcaaataa tatttaccca cttgaattgt
     1021 atcacataat attaacctta gaaacaaact ttaatattat tatataataa attatttttt
     1081 tattatgaat gaggcattag aagataacaa ctacttttag ttgctacttt ttaatttatc
     1141 tattaataaa atctaatgct ttacaaagtg tatcaaatct atcattacct tttatcattg
     1201 tataatgttc tttagtaata gaacttatct tttcacatgc tccaccacct atgacataaa
     1261 gattttgtgt ctggccaggt acataatctt ttatatcaca tatcagtatt ttcccatcat
     1321 tataacccca accaactaca gttgcaggga ttttgtcaac ttctccatca taaacaattg
     1381 tatgtttgta catctgttta actccctcat tatttatatt tttatttaat acaccttcta
     1441 caattaactt agcaatacct tcatgaccta gtttcttagc tttatcataa tcttctttat
     1501 tatcacagaa gaaactttca attaatactg ctgtaggctt tgaactattt aagatatata
     1561 atcttttatc taatttagct cttctatttc taaatactgt acctagctta ttacatattc
     1621 tagttgcata ttctaaacct ttattactat aatatagaac ttctgaacct tttccttgac
     1681 catcacttgc atttagatgt agttctataa gtaaatcata tcctccacta ttaactctag
     1741 gtattttata aatcttttct tctgctttag ttttaaactg cttttcaggg catattatta
     1801 catctgcctt atgaccttct tttctaaatg tatctgctaa tactggtgca agaaatttat
     1861 tgtattggta ttcgttaact accccatcag cagaagtgca tgcaccactt tttaaaatac
     1921 tgtgtcctac tgtaatacat attttcatta tttattttcc tccttctttt caataaaaaa
     1981 acacttacat atagtaagtg tttataaact tcttaaagta actattttat ttagccaata
     2041 tctctatcca attattagga aaaccaataa gctttaagtc tatagaatta ttataatcat
     2101 taattaactt ttgaagtttt ataaaaaaac tattccaaat taatttatcc atagtcaaat
     2161 gcttcatagc aagtatataa gaaa
//
LOCUS       DAEMAE010000353         2179 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEMAE010000353 DAEMAE010000000
VERSION     DAEMAE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.360"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..499
                     /locus_tag="KPH93_003634"
     CDS             <1..499
                     /locus_tag="KPH93_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG1690395.1"
                     /translation="EEVGSSTKQGADSNMLLNILERICISMGKDREGFFESIYSSFMI
                     SSDLAHAIHPNIPEKHDPTNKPIMGKGPVIKINASQAYTSDGYSIAIYKNICREANVE
                     YQEFVNRSDERGGSTIGPISSTHIDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFS
                     KFFEI"
     gene            530..1282
                     /locus_tag="KPH93_003635"
     CDS             530..1282
                     /locus_tag="KPH93_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG1690396.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILVSTKYNYELPEDIKKIYDEDKIIVVRPNTQ
                     FAPKDTLNFDDKFCKTIYQEGYEIGKNILDRL"
     gene            1347..1871
                     /locus_tag="KPH93_003636"
     CDS             1347..1871
                     /locus_tag="KPH93_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1690397.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
BASE COUNT          876 a          220 c          390 g          693 t
ORIGIN      
        1 tgaagaagtt ggtagctcta ctaaacaagg tgcagattca aacatgttgt taaatatatt
       61 ggagagaatt tgcatatcaa tgggtaagga tagagaggga ttctttgagt ctatttattc
      121 atcttttatg atatcatcag atttagctca tgctattcat ccaaacattc cagaaaagca
      181 tgacccaact aataaaccaa taatgggaaa aggtccggtt ataaagataa atgctagtca
      241 ggcatatact agtgatggat attctatagc tatttataaa aatatatgta gagaagctaa
      301 tgttgagtat caagagtttg ttaataggtc agatgaaaga ggaggaagta ctataggtcc
      361 aatatcttct actcatatag atataccatc tgtggatgtg ggaagtccaa ttttagcaat
      421 gcactctata agagaattag gaaatgttga ggaccactat agtatatata aaacatttag
      481 taagtttttt gaaatataaa tataagataa acaatttgga gatgagacta tgaaattagg
      541 attatgttta gaaggtggag gagcaaaagg ggcttatcaa gcaggagtag ttaaagcctt
      601 atatgatgga ggaataaata aattttattc gatttcaggg acatcaatag gagcaataaa
      661 tggatattat ttgtacacag gaaatgttga taacttggag aaaatgtgga caaatataaa
      721 agatatccag aatggaaatg taaaaatagt aaataatact gtggataatt ctcctgccat
      781 agacaaccta agggaactag atgatagtaa tatagaagaa atgaactttt atgttaatta
      841 tgttgaagtt gataataaag tagtatcaga aaagattgtt gatgtatcaa aaatgcctcg
      901 taatgaagca attatcagca tttcatacag tgggttgtta ccatcaaatc caaatgcaac
      961 tcttggattt aaagaacaat ttgtgaaaga tgtacaagaa ggaatttatg atggtttcaa
     1021 attagatgga ggtttaatta ggagtgcatt aattgaacca ctaattgggg ataatgtgga
     1081 taaaattatt ttagtatcca caaaatataa ttatgagttg ccagaggata ttaaaaaaat
     1141 atatgatgaa gataaaataa tagtagttag acctaatact caatttgctc ctaaagatac
     1201 gcttaacttt gatgataagt tttgtaaaac tatttatcaa gaaggatacg aaataggaaa
     1261 aaatatactt gatagacttt aatttagagt ataatataat aaatgtaatt atgtaattta
     1321 actaatgaaa aggaaggtaa taaattatgg aaaataaaaa tcctatagta actatagaaa
     1381 tggaaaatgg aaaagaaatc aaaattgagt tatatccaaa tatagctcca aatacagtta
     1441 aaaactttgt atcattagta aatgaaggat attacaatgg aataatattt catagagtaa
     1501 taccaggatt tatgatacaa ggaggatgcc caaatggtac aggaatgggt ggtccaggtc
     1561 actcaataaa aggtgaattt agtggtaatg gatttactaa caatttaaaa catgaaagag
     1621 gagtcatatc aatggctaga actatggcac caaattcagc tggttctcaa tttttcataa
     1681 tgcataaaaa ttcaccacac ttagatggtc agtatgcagg ttttggtaga gtaatagaag
     1741 gtatggatac agttgatgaa atagcatcag ttagaacaga ttcagctgat aagccacaaa
     1801 ctccacaaat tatgaagtct gttactgtag aaacttttgg aatagattac tcagatgtag
     1861 aaaaaaacta aaacttatat aaaaactagg ttgagttata aataaaaatt aaagatatat
     1921 attacttaat aatgaggata aagtgaaatg ttagtatgtt tttagacaaa tacatttcac
     1981 tttttaattt atttgtattg tggaaaacta tatattgtat gattttaact aaaaaataaa
     2041 gttttattaa aaaaatttta ttatgattgt ttatatattt atttttaaat aataatgtat
     2101 taaataaata tataagatat gtggaaaaca cttatttcag tattttttaa acttatttta
     2161 tttattctat ttaataaaa
//
LOCUS       DAEMAE010000354         2136 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEMAE010000354 DAEMAE010000000
VERSION     DAEMAE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.361"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..1832
                     /locus_tag="KPH93_003637"
                     /pseudo
     CDS             206..1832
                     /locus_tag="KPH93_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          913 a          237 c          277 g          709 t
ORIGIN      
        1 aaattctata ttaaatacta aatctatatt ttctacatta aatactaaat tcatatttaa
       61 atactaaaac cattcattaa tatttaccca aatttataca aattaaaaag gggctgtcgc
      121 actaaaaaat agtgctaagc ccttttttat ataaaaaaat aaatcccaag ttcaaaagag
      181 cctgggattt ttgtataata attgtatgtc aaaacaagat actatacaaa ttgattatac
      241 tactcatggt gataattatc aatttaaatt accactaaat attgattata tgattccaaa
      301 agatgattct gtaaggctat taagtcaaat tattgaggag atgaatttag aaaagttata
      361 taagacttat tctcgaatta gaggaaatag agtaacccct agacaacttt tgaaaattat
      421 aatatatgca aatatgaact gtatttattc ttcaagaaaa atagaacaag catgtaaaag
      481 agatattaac tttatgtatc ttttaggcgg agcctccgct ccagaccatt caacaattgc
      541 aagatttcgc agtattcatt tttcacaagt aagcaaaaat ttgcttgcac agtttacaaa
      601 ctttcttgga gataataagg ggatttcaaa agatgttcta tttattgatg gtacaaaaat
      661 agagtcatct gctaatagat atacttttgt ttggaaaaaa tctataaaca aaaatatggt
      721 tagaataatg aatgatatgt ctaatttcat attaaaatgt gagattgatt ttggtattaa
      781 aataatttat agaaataaaa tcaaaatata tcatttaaaa aaacttttaa agaaacttaa
      841 aaaaatatca aaagaatcaa atataacatt tgttcatgaa aaaggaaaga aaaaatctat
      901 tgttcaaaaa tctatagaga aattaaataa ctatatcgat agattgaaaa atatactaaa
      961 gaccttcata taatgggtga acgaaatagt tattctaaaa cagataatga tgtaactttc
     1021 atgagaatga agaaagacca tatgaaaaat aggcaattga aaccagctta taatatacaa
     1081 tttggtgtag attcagaata catagtttgg gtaagttcag agcattaacc tactgatact
     1141 acaacattaa taccattttt aaaatctatt gaagaccata caaaatttaa atatcctaaa
     1201 atagtagctg attctgggta tgaaagtgag gaaaactata catttttaga gtgtaataat
     1261 caattatctt ttataaagct tgcaaattat gaaattagta aaactagaaa atataaaaat
     1321 gagattgggc gtattaataa tatggattat gatgaaaaaa atgattatta tatttgtaaa
     1381 aatggcaaaa aactatttgc taataaaata atcaatagaa aatcaaaaac aggatataaa
     1441 agtaaaataa cttgttatac ttgtaaagaa tgccgtgatt gttaatataa aagcagttgt
     1501 attaagggaa ataatagtaa gattcctctt gaaaatagaa ctaaaaattt acaagtatct
     1561 aagttatttc atgaaaaaag aaaagaaaat ttaaagagaa ttataagccc ggaaggttgt
     1621 caacttagaa taaatagaag tatacaagca gaaggagctt tcgctcaagt aaaaaaaata
     1681 tgaaatttag aagattttta agccgaggta aagaaaatgt tctatcagaa tgtattattg
     1741 tagcgatagc tcataacatt tctagactac acaataaaat aataaaaaat aaacgtggaa
     1801 tatatcttca tcatttaaaa gaagtattct aaatttatta tttatagtta aatattatct
     1861 gaaaaatggt atttttatac ctttatttgt tatgcataat tttatttaga acataagctc
     1921 tatataaaac aattaaaaat atacccatta agataagtaa aaaagctatc acagaacaaa
     1981 tttaatttgc taatgcgaca tccccattac tttttttatt tttgatttgg atattctagc
     2041 atttggtttt aaatcttcaa ataatagttt attagtcatt ttttacctcc tttcatgttt
     2101 tattttacat acataataaa atataagcat atatta
//
LOCUS       DAEMAE010000355         2089 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEMAE010000355 DAEMAE010000000
VERSION     DAEMAE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.362"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(99..335)
                     /locus_tag="KPH93_003638"
     CDS             complement(99..335)
                     /locus_tag="KPH93_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690398.1"
                     /translation="MLLTHIYFNGQFNQTIELYKQTFNATVKTLIEDSGHLLIVHNEI
                     VIHNKFLILSDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(491..715)
                     /locus_tag="KPH93_003639"
     CDS             complement(491..715)
                     /locus_tag="KPH93_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1690399.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(812..2065)
                     /locus_tag="KPH93_003640"
     CDS             complement(812..2065)
                     /locus_tag="KPH93_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG1690400.1"
                     /translation="MITLTFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSQTITPSIFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGASPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT          797 a          313 c          237 g          742 t
ORIGIN      
        1 tttaaatact tattcaattt tatctataca taaacaaaaa aagagatttt aaaatctctt
       61 tttagattgt tgacttatgg ctttttgatt taataaaatc aagaagccgt ttgaatttta
      121 tctgttggct gatacccttc tgatattgaa acaccatttt tgtttccaaa atcgcttaaa
      181 attaagaact tattatgaat gactatttca ttatgaacaa taagcaaatg acctgaatct
      241 tcaatcaatg ttttaacagt agcattaaat gtttgcttat acagttctat cgtttggttg
      301 aactgaccat taaagtaaat atgagtcaat aacataacta tatctacaaa aattaatata
      361 aattttttat actgtacatt atctaactgt tgattatctg tattacaaca tgattgaatt
      421 taaaaagcca tcaacacata gaatgtcgat ggcctgaaaa tagattttaa aatctctttt
      481 ttattatata ttaattattt tttaaattta tattttttgc atcagatttt ctaatagcta
      541 agtcaaaccc tctaaatcta atcactatag ggtcattaag aggagctatc tttttcactt
      601 caacttcagt gccatctatt aatcctaagg ctagaagtct cttcataagt ttttcatttc
      661 cagctatatt gtctataatt cccttttgtc caagtttcaa gttgtaaaca gtcattatta
      721 acacctccta atgaaaatca ttatcaaact ttatatttat aatatataac aaaaagagca
      781 aattgtcaat atataacaaa aaataaacat actaagttag tatgtttata aaaaatagta
      841 tacatgggat actaatagca tctagcaaaa aaccacttac taatggtaca accaaaaagg
      901 cttttggaga agcaccatat ttttgtgtta aagaactcat atttgccaaa gcgttcggag
      961 tagctcctat tccatgtcca atcaaaccac ttattatgat ggcagaatca aaatcttttc
     1021 caagtactct aaagcaaata aatattgaaa ataggattat aaagattact tgaaaagtta
     1081 ctattaagag aataggaggt aataatgtag acaattcaaa taaatctata ttcataagcg
     1141 ataaagttaa aaaaattcct aatgaaagtt cttttaaaaa atctataagt ttaaaattta
     1201 attctagtat atttatctta tcatttaaat ttctaaaaat aacagctgaa aacatacaag
     1261 ttacaacaga gggtaatagt atattaaacg ttatatagaa gcatctactt ataatttctc
     1321 ctgtatttat acaaataaat atgagaagaa cctgttccaa aaatatagat ggtgttattg
     1381 tttgggagaa gttattattt tttatcctat tcttatttct aaaaaattta cttccaaatc
     1441 tctttagatt tctattatat ctaggaatcg acaagtctat atttgatgat ggttttaatt
     1501 tatatttatt tattagaaat ttagctacag gacatcccaa tataccaccc ataataagtc
     1561 ctattgttgc agcagataat ccaaccccaa ctgctccttc tataccgaga ttttctattg
     1621 taagtccaaa tgcagcagaa taaccatgac caccttccat agatacattt ccacacataa
     1681 gccctaaaag aggttcaagt tttataatct ttgatattgt aatagctaat atattttgac
     1741 aaaatgccaa tactccacac aaaatccaat atctaaataa tacactacca cctttcttta
     1801 taagatttat gcttatttca agtcctatag ttgtaaaaaa gaaggttata aaatttggca
     1861 ttaaatttcc acttatagat atatctgcta tattaaaata tttaaaaaat aaatttagaa
     1921 aacaaaataa tagacctcct attactggtg caggtataca gaaattgttt aaaaagttaa
     1981 ctttattctt caataaatta ccaattaaaa agaagaatat agatagtgtc aaagtttgca
     2041 ctatatcaaa agttaaggta atcaaaataa gtcctccttt gtaattcaa
//
LOCUS       DAEMAE010000356         2041 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEMAE010000356 DAEMAE010000000
VERSION     DAEMAE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.363"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..986
                     /locus_tag="KPH93_003641"
     CDS             129..986
                     /locus_tag="KPH93_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1690401.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVESKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQRVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1083..1952
                     /locus_tag="KPH93_003642"
     CDS             1083..1952
                     /locus_tag="KPH93_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1690402.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELRELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKVVFRSNHASNYVNLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          850 a          194 c          366 g          631 t
ORIGIN      
        1 aaacaaaaaa catatattta tgaattttat atgaaaatag ttaaaatatt gatttttttc
       61 attggttgaa aaaacaattt acttaggata aactataata gtattcaaat attatggggg
      121 ttagatttat gagtaaagat gtaaaggtaa agcgtaataa gaaatatgat acatttagta
      181 taactatgga acttgagaga cctttacttt atgatgaggt tgagagcaaa gatgataaaa
      241 ataataatag aaaatttaac ataaagaata agatatttaa gggaataggt atactatttg
      301 tattgatttt gcttggtttt ttcatatgga taaataaaac atatgaacct caaaagctag
      361 caaaagaagc tctagtcagc aatagtaaag ttgaagtaac tgtaaatgaa aatatttcat
      421 ttacaccaaa aggaagaaaa gtttccaaag gtttaatttt atatccagga gctaaagtag
      481 aaatagaatc ttatgctcca cttgcaagaa aaatagcaga aagtggatat gaggttgtaa
      541 tcgtaaagat gccacttaat ctagctatac ttggaactaa taaggcacag agagttatgg
      601 attcttacaa caacatagaa cattgggtaa taggaggtca ttcattagga ggagttgcag
      661 catcaaattt tgcgcgtgat aataaattaa ttgaaggtgt tgtattttta gcttcttatc
      721 caatgggtga tgagttgaaa gaattaggaa aaaaagttat atctatatgg gggagcaaag
      781 atggagtagt taattttaaa agtcttgtag aatcaaaaca aaaacttcct gatgatacaa
      841 cttatgttga gattgaagga ggaaatcatg cacaatttgg agactatgga aaacaaaaag
      901 gagataatga tgctattata agtcaagaaa aacaacttaa tattactgcc aatagtatca
      961 ttaaattttt aaagaatata tcttaaacta aaattttaaa aatataattt tataagttat
     1021 tatttaaagt aaatattaat aatataatag aatgaatatt ttattacaag ggggatttta
     1081 atatggaata cgatatgcca ctttacagac caccaagtga ggcttatagt cttataatac
     1141 aagtcacact aggttgttct cacaacaaat gtacgttttg tagtatgtac aaatctaaaa
     1201 aatttaaaat aaagccacta gaagtaataa aaaatgaaat tgatatattt agaagatatt
     1261 ataaacacgt agaaagaata ttcttagcag atggagatgc attgataata ccaatggaga
     1321 aattaagaga tattatttta tatataaaag aagtttttcc agagtgtgaa agaattacta
     1381 tgtatggatc tcctaaatct atagagaaaa aatctgatga tgaacttaga gaattaagaa
     1441 aacttggtgt taaaatgatt tatctagggc ttgaaagtgg aagcaatgaa gtattagaag
     1501 atattaagaa aggttttagt agtgaacaat taataaaagt tggtagaaaa gttaaaaaaa
     1561 gtgggattaa attatcagcc actgtaatag caggtcttgg aggaactaaa aaaacacatc
     1621 agcatgcagt tgatacagga aaaatgttag gagcaatttc accagattat gtgggtgtac
     1681 ttagtttaat ggtagaacct aatactgaac tttatgattt acttaaggct ggagagttta
     1741 caatactaga ggataaggct gtgttacaag aaattaaaga gatgataaag aatatagatg
     1801 ctaatgaaaa agttgtattt agaagtaacc atgcatcaaa ttatgttaat ttaaaaggtg
     1861 tattacctga tgataaacaa cgtctaattg atgaaattga ttactatcta agtaaccaaa
     1921 agctaaaaag agaagaatat agaagactat agtttttgat atatcaatct atattgatag
     1981 gtaaatactg agattgttta gatataaaaa ataaaatggt atattcatat ataatctatc
     2041 a
//
LOCUS       DAEMAE010000357         2025 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEMAE010000357 DAEMAE010000000
VERSION     DAEMAE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.364"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      <1..69
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            122..298
                     /locus_tag="KPH93_003643"
     CDS             122..298
                     /locus_tag="KPH93_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690403.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(412..768)
                     /locus_tag="KPH93_003644"
     CDS             complement(412..768)
                     /locus_tag="KPH93_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690404.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            1024..1944
                     /locus_tag="KPH93_003645"
     CDS             1024..1944
                     /locus_tag="KPH93_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBG1690405.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNKLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVNIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHDKKTLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGKMLV
                     KVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
BASE COUNT          764 a          271 c          275 g          715 t
ORIGIN      
        1 agaaatttag tgacgcaaag ctatagggac taagacttat gaaatgagtt atgtcagcca
       61 gttgccaaag agatattttc tttttgtttt tatgaatgtt ttttaggggg atatatgata
      121 tatgtttatg gataaaatgg atagatgtac acatatacta actgcatata tatgcagttc
      181 acgcgattac tgcaatttta tagatacaca attaaatgat tttatactag aatatggaga
      241 gaacgtggta gaatcttgtt tgcatcaagt gatggtattg gtaagcagat ataattaaag
      301 aaattttatt ttctagaaaa ccctttttag ttttatctac gttttataat tcattaaaga
      361 actaattaaa ataagcttca ttatctattt tcatgaagta aacagttgag tttatctcat
      421 atactgttta ccccaattac ataattctaa caatatagat tccaatgatt taccacgttt
      481 agtagacaaa tattcaactt ttggaggaat agtaggataa gaaatcctct taataagtcc
      541 atctctttca agatacttta attcttgtga cagtatctta tgacttatat gaacaacttc
      601 tttttttaat tgactgtaac gtattggctc ttgttttaaa atatgccata agataattgc
      661 cctccattta cttcctatca cagtcatggt aaaaccaatt gaacaattaa aatctttttg
      721 tctttcttca atatttggaa attcttcaaa attattatct atatccatct caatcctctc
      781 tttcaaaata gtaagtatct aactaaaaag tgcattcttg tttagcgtac taattcgtaa
      841 tataatcttg attgtagcat agagccttac ataatactac ttttttagat gtatatgttt
      901 aaattttaat atagatatat gttattttat taattttatt taaacagata ataataaata
      961 ataatttatc tcaagttttc tatgtacata aactaatcta atgtttagaa aggattatat
     1021 aatatgaata aaaaatttcg tctttatcaa gttgactcat ttacaaaaga aaaatttaca
     1081 ggtaatccag ctggtgtaat tacaaatgct gatggcttat ctgaatcaca aatgcaaaaa
     1141 atagcaagag aattaaataa ttcagaaact gcttttattt tcaataaatt ggctaaaaat
     1201 catcaagaaa attatgatgt acatatcaga ttttttacac caactaatga ggttcctata
     1261 tgtggtcatg ctactattgc tgcccattat gttagagcag tagaaaacca attcaataac
     1321 aaaacagtat ttcaaaaaac tggtgctgga atattaccag ttaatattat aaaaaaagat
     1381 aatgattatg aaataattat gacacaagga aaaattgaag tatctccacc attacaacaa
     1441 acctatcaaa aaagattact taatgcattt ggtattttaa aggaagatat tcgtacagat
     1501 tgtcctattg ccatatcatc tacaggacat tcaaaagtta tgattggaat aaaggaatta
     1561 tcaacattaa atagtttatc tccaaattta tatgaattat cgaaaataag tcaagaaata
     1621 aaatgtaatg gatattatat ttttacatta catgataaaa aaactctaca agttcatgga
     1681 agaatgtttg ctcctgcaat tggcatttca gaagaccctg ttacaggaaa tgctaatgga
     1741 cctttaggtg cgtaccttgt acattataaa gtaataccta tacccaacac agagtttata
     1801 tttgaagcag ttcaaggaga agcaataaag cgcaaaggca agatgttagt aaaagtaatt
     1861 atttcagata ataagccagt tgaagtaaaa ataattggaa aagctgtaat tgcattctca
     1921 acaaacatag aaattgaatt ataggttcct tctaagtaaa tttttgaact ttttaatact
     1981 aatttaataa caaactaatt tatatacaat caaattaatt ttttt
//
LOCUS       DAEMAE010000358         2021 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEMAE010000358 DAEMAE010000000
VERSION     DAEMAE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.365"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(93..254)
                     /locus_tag="KPH93_003646"
     CDS             complement(93..254)
                     /locus_tag="KPH93_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690406.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(597..1757)
                     /locus_tag="KPH93_003647"
     CDS             complement(597..1757)
                     /locus_tag="KPH93_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG1690407.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
BASE COUNT          742 a          353 c          200 g          726 t
ORIGIN      
        1 aactttatca caaaaaaacc taacttaagt atttatattc tacataagtt aggttttata
       61 aactattata atatttatta tttcattcat ttctagtgat gattctcctt ttttatcttt
      121 ttcaatttgc cttcactaac aacaaataaa aatcccatct tcttgtatat ttcatccaat
      181 tcaattaaag ttacttcacc attttttaaa tttgttaatg ttatatcttg tattcttctt
      241 gatggaatta tcataagtta tcaacactcc tttaaattct ttttaatctt tatatttaaa
      301 tatatttatg cattctgata tatattcata aattattaaa ataaattcaa aaaacacccg
      361 ttctgcaaac attcgttctg tatatttatt atacatcata cagacgttcg attcaatata
      421 aaaattaaat atatctcgtt tttagtaaat tttacaatat tatacactaa atatgtcaaa
      481 atatcttctt gaaataaatt atcttgtcaa acaaaaaaag ccatttcaat aaaagcttct
      541 gcttaaattg aaatgacttt tcttatttaa attattaatt ttataccata tttttattaa
      601 gctaacatat gtagtatttg agttaccata agagcacaat aagtccctat aatgtttcct
      661 agaagcccca taagtacacc tactggtatt aatacaggat tgtgaacccc agcaatcatt
      721 ggagctgtag ttactcctcc tatattagct atacttccta cttcacaagt aaataaatca
      781 agtttaaata gtttagcaag ccctataaat gaaatagcat gtacaaggac tactaccaat
      841 ccaaacatta tgtaaacagg tgcttctgac aatccaaaga aatttgcttt actccctgta
      901 gctgcaagga gtaaatacat aaacacattt cctaccagtg tactcccctt aagctttgat
      961 atttttgtca tacccaataa tattgatact gtagatgcaa caagtactac ccacatagtt
     1021 tcattaagta tacctgaagt tggaagtatc ttggatacaa tttgtgcaaa agacgcaact
     1081 ccaaacccaa cagaaagtaa aatcataagt tctacaaacg taggtgctgt attatcttta
     1141 ttttcttttt caagttttga tgtaacctca tctataaaac ttgtatcgca ctttgtccat
     1201 ttgttaaatt taggaccaaa tttcacagac attagacaaa cagacatcca tactgtatat
     1261 cctatagaac ccataagtat tgcataacta agtccttctt ctgcaactcc tagagcatta
     1321 gctgctgcaa tcatatttga tgagccacct atccatgagc cagatgctgc cccaagaatt
     1381 ttccatgaat ctgcacccat tgaattttta aaaattatga atgctataac aaatccaagc
     1441 ataaatgtaa atgtagcaca gaaaaatgat aataacattt taggccccat ctttagtatt
     1501 tttcttaaat cacattgaat taacaagaat actaacatta atggaaggaa gttcactgtt
     1561 attatatttt gatacgttcc aacatcttca ttctgtgcaa ataaaccaaa tgatccgagt
     1621 atagctgaac ctaaatatat taaaagcatt ggattaataa catcaaatac cttccatttc
     1681 aatttatcaa ccataaaaaa aattacacct acgaatacaa ccaagaaact acaatataca
     1741 aatccagtct caatcacatt atacacaccc tttcttttcc taattaattt gattttttaa
     1801 tgatttttaa gtaaagactt tttcagataa tagacaatta aaataaatac ttttatctat
     1861 atcatgctaa ttaaatacat atatacctag ctattgacat atattaataa agatttgcat
     1921 attaattact ttagaaatga ataatctgaa tttaaaatct ttttcaacaa ttattttatt
     1981 actaaaattt ttatttgtaa agataattaa actaaatatt t
//
LOCUS       DAEMAE010000359         2019 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEMAE010000359 DAEMAE010000000
VERSION     DAEMAE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.366"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..409
                     /locus_tag="KPH93_003648"
     CDS             35..409
                     /locus_tag="KPH93_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1690408.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            406..1269
                     /locus_tag="KPH93_003649"
     CDS             406..1269
                     /locus_tag="KPH93_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690409.1"
                     /translation="MISMDAITIKNLNKTYKNFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGSIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWSDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHNPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1269..1907
                     /locus_tag="KPH93_003650"
     CDS             1269..1907
                     /locus_tag="KPH93_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1690410.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFTGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLSIILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          761 a          215 c          308 g          735 t
ORIGIN      
        1 atatactata tatacaattt aaggaggtgt cttattgaat atagttatta gtaattcgag
       61 tgggaagcca atatatgaac agattactac acaaattaaa agtatgatta taagtggaga
      121 gttaccagaa ggctcagctc ttccaagcat gagactattg gcaaaggaac ttagaataag
      181 tgtaattact acaaaacgag catatagcga tttagaacga gacggattta ttgagacagt
      241 tactggtaaa ggcagttttg tatctagtaa aaacatggat tttgtcaaag aagaacaatt
      301 tcgtttaatt gaagaatacc tacaaaaagc agttgataca gcaaaaagta gtgatattac
      361 atatgacgaa ttaaaagata ttttattttt attatataaa ggagaatgat aagtatggat
      421 gcaataacta taaaaaattt aaataaaact tataaaaatt ttagcctaca agatatatct
      481 ttttcagtac caaaagggag tgtaatggga tttgtaggtg aaaatggcgc aggaaaaaca
      541 actacattaa aagcaatttt aaatttaata tcttatgata gtggaagtat agaaatcttt
      601 ggtcttgata gtaaaaaaaa tgaaaaagaa attaaagagc aaattggggt tgtatttgag
      661 ggaagcaact ttcatgaaaa tttaaacact gaccatgttt caaaaatcat gagtaaaatc
      721 tataaaaatt ggagtgacac attatttaaa gactacttaa aaaaattgag agtaccagac
      781 aataaattaa ttaaggagtt ttcaaagggt aataagatga aacttagtat tgctgtagcc
      841 ttatctcata acccaaaatt attaatacta gatgaggcaa ctagttcact tgaccctatc
      901 gttcgtgaag aaatactaga tatattttta gattttatac aagatgaaga acactctatt
      961 atcttatctt cacatattac aagtgattta gataagatag cagactatat tacatttata
     1021 cataaaggaa aaattgtatt tagtgaaaat aaggatgaat taattgatac tatgggagtt
     1081 ttaaaatgta aacctagtga ttttgataac ctttcaagag aggattattc atattatcgt
     1141 aaaagccaat ttggatatga agtacttctt aaagataagc atagatttat ctcaagacat
     1201 ccaaactgta ttgttgataa tacgtctata gaagaaatta tgttatttta tgtgagaggt
     1261 gataaataat gaaaggtcta attttaaaag atttacttaa tttgaaagga aatgtaaagt
     1321 ttatactttt attcatcatt atgtttggct ttatgtcttc tttaggagat ggaaatgtta
     1381 ataactttat aggtgttatt atagttttat gtactacaat gattgtttca acattttcat
     1441 atgatgattt aaataaatgg gatagttatg tgcttacaat gcctattaat cgcaatgata
     1501 ttgtattaag taaatattta actatgttaa tatttagctt cacaggtgtt cttgtttcac
     1561 ttattgttag tgtaactatt ggttatttta aaaatacact tatattaaat gaaactctac
     1621 ttattaatgc actaatctta tcaatttcag tatgttttgg tagccttatt ctaccactta
     1681 tttataaatt tggtacagaa agagctcgtt tatcaataat tttatgcttt ttagttccaa
     1741 cactagcttt attagttttt aaaagtattt tagaaaatat tagttcacct atttctatag
     1801 aaattatact taatacacta gtatattctc ttccatttgt tgctatttta ctatttgtga
     1861 tttcgtattt tatatcatca aaaatctata gtaaaaaaga agtataaaat atactattat
     1921 atgtttctgt atttaagtat ttaaataaaa tactatcgag tttattttgg tagtatttta
     1981 ttttttaatt attaatatct actaggcatt tattatttt
//
LOCUS       DAEMAE010000360         1961 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEMAE010000360 DAEMAE010000000
VERSION     DAEMAE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.367"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..633)
                     /locus_tag="KPH93_003651"
     CDS             complement(64..633)
                     /locus_tag="KPH93_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1690411.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            836..1063
                     /locus_tag="KPH93_003652"
     CDS             836..1063
                     /locus_tag="KPH93_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1690412.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1282..>1961)
                     /locus_tag="KPH93_003653"
     CDS             complement(1282..>1961)
                     /locus_tag="KPH93_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG1690413.1"
                     /translation="FERSKYFSYDVKYPVIYNIRNQSTYVNPVILNNINNAVRMTVNN
                     FKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTFNKNYVLSMILNLMGFANNYGPK
                     YNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDYIKSQINENREMYYSNVELVIPE
                     DQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSDFGNFINLRFYCISPDIYVQSRG
                     RAKHFMG"
BASE COUNT          668 a          286 c          250 g          757 t
ORIGIN      
        1 agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata aattatatct
       61 ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag tcatcttcac
      121 aacctctcta tatggcttag aatttaataa tcccaatgca tattgtatct gttgattgct
      181 gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt cttttcctaa
      241 ttgagtacgt tctattatca tatttctttc aagttctgca actgcaagta atgtagtcaa
      301 aaagaattta cccattgaag tattttctaa aagacctata tttaaaatat gaacacttac
      361 ttctttagca aataaacctt ctattagttg tatcccttct gatgtacttc tagctagtct
      421 atccagctta gttgcaacaa gcaagtctcc cttttttaga gatgatataa ttttttcaaa
      481 tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag catcattata
      541 tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt gattttttgt
      601 acttacacgg caatatccat atataataat catcacttcc taacataaag attttgtaca
      661 gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa cgatttttgg
      721 gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt ttgactatgt
      781 caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa ataatatgaa
      841 tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg aagttttttt
      901 attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata tcagattgaa
      961 attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg tagaggtcat
     1021 caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta aatgtatata
     1081 atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac aatcattcta
     1141 ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat agtattgttt
     1201 aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat actaaaatag
     1261 ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc ttgattgaac
     1321 atatatatct ggtgaaatac aatagaagcg taaatttatg aaatttccaa agtcagaaaa
     1381 cttcagttcg aacttaggta taccaaaact ttcaggtgct atttcgtcaa cgccaaaata
     1441 gatgataatt ccattatctg taagataaaa ggcttggtct tcaggtataa ctaactcaac
     1501 attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat cagtaattat
     1561 attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt ttccagttgt
     1621 caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat tagcaaatcc
     1681 cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg cgtaactggt
     1741 agatacccaa taactttgtt ttggaagtga attttgagaa gctgcgttat tatattctcg
     1801 ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag cattgtttat
     1861 gttatttaaa ataactgggt ttacatatgt ggattgattt ctaatattgt atataacagg
     1921 atactttaca tcatatgaaa aatacttact tctttcaaaa t
//
LOCUS       DAEMAE010000361         1937 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEMAE010000361 DAEMAE010000000
VERSION     DAEMAE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.368"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            130..246
                     /gene="rrf"
                     /locus_tag="KPH93_003654"
     rRNA            130..246
                     /gene="rrf"
                     /locus_tag="KPH93_003654"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            253..327
                     /locus_tag="KPH93_003655"
     tRNA            253..327
                     /locus_tag="KPH93_003655"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Asn,seq:gtt)
     gene            332..406
                     /locus_tag="KPH93_003656"
     tRNA            332..406
                     /locus_tag="KPH93_003656"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:365..367,aa:Glu,seq:ttc)
     gene            412..487
                     /locus_tag="KPH93_003657"
     tRNA            412..487
                     /locus_tag="KPH93_003657"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Val,seq:tac)
     gene            493..569
                     /locus_tag="KPH93_003658"
     tRNA            493..569
                     /locus_tag="KPH93_003658"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:527..529,aa:Asp,seq:gtc)
     gene            581..655
                     /locus_tag="KPH93_003659"
     tRNA            581..655
                     /locus_tag="KPH93_003659"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Thr,seq:tgt)
     gene            670..754
                     /locus_tag="KPH93_003660"
     tRNA            670..754
                     /locus_tag="KPH93_003660"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Tyr,seq:gta)
     gene            764..837
                     /locus_tag="KPH93_003661"
     tRNA            764..837
                     /locus_tag="KPH93_003661"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:796..798,aa:Gly,seq:tcc)
     gene            848..924
                     /locus_tag="KPH93_003662"
     tRNA            848..924
                     /locus_tag="KPH93_003662"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:882..884,aa:Arg,seq:tct)
     gene            934..1009
                     /locus_tag="KPH93_003663"
     tRNA            934..1009
                     /locus_tag="KPH93_003663"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Gln,seq:ttg)
     gene            1021..1096
                     /locus_tag="KPH93_003664"
     tRNA            1021..1096
                     /locus_tag="KPH93_003664"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:Lys,seq:ttt)
     gene            1099..1187
                     /locus_tag="KPH93_003665"
     tRNA            1099..1187
                     /locus_tag="KPH93_003665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1133..1135,aa:Ser,seq:tga)
     gene            1206..1296
                     /locus_tag="KPH93_003666"
     tRNA            1206..1296
                     /locus_tag="KPH93_003666"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1240..1242,aa:Ser,seq:gct)
     gene            1305..1381
                     /locus_tag="KPH93_003667"
     tRNA            1305..1381
                     /locus_tag="KPH93_003667"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Pro,seq:tgg)
     gene            1467..1542
                     /locus_tag="KPH93_003668"
     tRNA            1467..1542
                     /locus_tag="KPH93_003668"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1500..1502,aa:Trp,seq:cca)
     gene            1603..1679
                     /locus_tag="KPH93_003669"
     tRNA            1603..1679
                     /locus_tag="KPH93_003669"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1637..1639,aa:Pro,seq:tgg)
     gene            1685..1761
                     /locus_tag="KPH93_003670"
     tRNA            1685..1761
                     /locus_tag="KPH93_003670"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1719..1721,aa:Ile,seq:gat)
     gene            1765..1840
                     /locus_tag="KPH93_003671"
     tRNA            1765..1840
                     /locus_tag="KPH93_003671"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1798..1800,aa:Phe,seq:gaa)
     gene            1848..1924
                     /locus_tag="KPH93_003672"
     tRNA            1848..1924
                     /locus_tag="KPH93_003672"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Met,seq:cat)
BASE COUNT          490 a          443 c          523 g          481 t
ORIGIN      
        1 atttgatgtt cattcattaa gatatatgtg tagtttttag agtgttaact ctaaaaaaaa
       61 ataaaaaaag tattgacaaa aatagcaaaa aatgttaata ttatacttgt cctagcaaca
      121 agaaaaaaat gtggttataa tagcagagaa gatacacctg ttcccattcc gaacacagaa
      181 gttaagttct ctagcgctga tggtacttgg tgggaaactg cctgggagag taggacatag
      241 ccacgtgaat gttccaaggt agctcaacgg tggagcaacc ggctgttaac cggtaggctg
      301 tgggttcgag ccccaccctt ggagccatta tggctcgttg gtcaagaggt taagacaccg
      361 ccctttcacg gcggtaacag gggttcaatt cccctacgag tcaccatttg tgggatcata
      421 gctcagctgg gagagcacct gccttacaag cagggggtca caggttcgag ccctgttggt
      481 cccaccattt gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt
      541 cgagggttcg agccccttcc aggtcgccaa aataaaaaat gctggtgtgg ctcaacggta
      601 gagcagctga cttgtaatca gcaggttgta ggttcgattc ctatcaccag ctccatgtaa
      661 aactaatacg gaggatttcc cgagcggcca aagggggcag actgtaaatc tgttgtcatc
      721 gacttcggtg gttcgaatcc accatcctcc accaattaaa tatgcgggtg tagctcaatg
      781 gtagagttct ggccttccaa gccagctgtg agggttcgat ccccttcacc cgctccaaag
      841 aaagcgtggg tgcatagctc agctggatag agcaacgccc ttctaaggcg tgtgtccggg
      901 gttcgaatcc ctgtgcgctc accacatata tattggggat tagccaagtc ggtaaggcac
      961 atgactttga ctcatgtatg cgtaggttcg agtcctgcat ccccagccaa ataaaaatat
     1021 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
     1081 cgcggataga tcaccaatgg agaggtgtcc gagtggttta aggagctggt cttgaaaacc
     1141 agtgacttcg aaaggggccg tgggttcgaa tcccaccctc tccgccaaaa aaaatgactg
     1201 aatttggaga agtactcaag tggctcaaga ggatcccctg ctaagggatt aggctgggta
     1261 accggtgcga gggttcgaat ccctccttct ccgccattca aaaacgaggt gtagcgcagt
     1321 ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt cacctcgacc
     1381 aacttaagtt tttagttcgg ttggttagag tgcccaactc ataactagta attctagggt
     1441 ttgagtccat gaaggttatt cttcataggg gtgtagctca gttggtagag cgtcggtctc
     1501 caaaaccgtg cgccgggggt tcgagtccct ccacccctgc caaataaaaa ggacttgaaa
     1561 aaagttggat tttagagtaa tataagatag taaataagat ttcgaggtgt agcgcagttt
     1621 ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa
     1681 gaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag gtcgctggtt
     1741 cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca ggggactgaa
     1801 aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc ggtatagctt
     1861 agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc tattaccgct
     1921 accatctaac tatatca
//
LOCUS       DAEMAE010000362         1923 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEMAE010000362 DAEMAE010000000
VERSION     DAEMAE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.369"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            233..526
                     /locus_tag="KPH93_003673"
     CDS             233..526
                     /locus_tag="KPH93_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1690414.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            507..1148
                     /locus_tag="KPH93_003674"
     CDS             507..1148
                     /locus_tag="KPH93_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG1690415.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGMILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1392..1724
                     /locus_tag="KPH93_003675"
     CDS             1392..1724
                     /locus_tag="KPH93_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG1690416.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          743 a          173 c          302 g          705 t
ORIGIN      
        1 taggttttta ctttaacaat tgtggtgtaa tacacttttg ttaaattaaa aaaattagat
       61 aaaataagaa ttaaataaaa gcgacttaag taaattgaga atcttaatat aaaaatttta
      121 aataagtatc tagaaatagc tatttcgaaa tttttctaaa tagctattga aataaaatgg
      181 atgttatgtt atattctatt tagatgaaat tctaaatagc gaggtgatgg tgttggggtt
      241 tccagaaaca ttcaaagctc tttcagaccc tataagaaga gaaatattgg ttatgttgag
      301 ggagaataaa atgtcagcag gagaaattag tgaaaaattt aatatgacga atgctacaat
      361 ttcttatcat ctatctcaac taaaaaaggc aggactcata tttgaaacaa agtataagaa
      421 ctttattttc tacgaaataa atatttcagt tttcgaagag gttatgcttt ggttttcaca
      481 atttatggag aagggagaaa aagataatga aaaaagcgat aaataaacaa tttattctat
      541 ctactttaat atgttttata ccatttattg tatcaatata tttttataat agattaccaa
      601 atgaagttgc aattcatttt gacaattatg gaaatccaga taattatgct ccaaaggtta
      661 tagcagcttt tggtgtacca ttgttaatgc tatgtatcca tttgtataca tggtttagac
      721 ttgagaatga gcctaagaaa gctaattttt caaatgttat acaaaattta tcaaaatggg
      781 gagttgctat actttcagta attctacaaa taatgatggt tttatattca acaggagtta
      841 gtttggagtc taatttttat acaggattaa ttttaggaat aatagttatt ttatttggaa
      901 actatcttcc taaatgtaaa caaaattact cagtaggaat gattttacca tggactctta
      961 atgatgaaga taattggaat aagacgcacc atttagctgg atggatttgg cttattggtg
     1021 gaatattatt aattattaat gcatttatta atattccttt ttataatatc tttgtaatct
     1081 ttgtaattgt aatactacca tttatatatt cttatttgat gtataaaatt tcaggtaaaa
     1141 ataactaata taattaatca ctataataat atttggataa gaataatata tttcagaact
     1201 gagatagata ataaagaaat tactaaggtt tctattttag ttctgaaaaa atcttactta
     1261 tatgataact tgttgagatt tcaaaaaata atataaatta ggctaatgaa cataatatga
     1321 aaatttgtat ttaatattga aaatataagt acagtgtgat attcttataa gaaaaacata
     1381 ggaggtagaa gatgaatact agagaaatga aagaatatag gggattttac aataaaaaat
     1441 taagcgaaac agatgagttt tttagagtat ttggaagtct tgatgataag acttattcag
     1501 aaggggctat aagtaaaaaa aataaggaat taatgggact agctatttca attttaagcc
     1561 gatgtgatga atgtatttgt tatcatattg agggttgtat taattctgga gcaagtaaag
     1621 atgaaattat agaagctata aaaataggtg taattggtgg cgggtctatt acatatccaa
     1681 acgcaagatt tgcaataaaa attttagaag aatttaatat ataattttga tagaagtagt
     1741 ttatttagac taataataat agtctattta agtctttgaa attaagcttt attttctaag
     1801 ttcacttctt attaagtttt tagaattgaa catataaatt ttaaaacaat atagtagttt
     1861 taaattattt acatcataga ttgtattgag aatagtatat tgttattatt aaatcttttg
     1921 tac
//
LOCUS       DAEMAE010000363         1916 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEMAE010000363 DAEMAE010000000
VERSION     DAEMAE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.370"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(94..645)
                     /locus_tag="KPH93_003676"
     CDS             complement(94..645)
                     /locus_tag="KPH93_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG1690417.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMHISYKMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(797..1060)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1496..1894)
                     /locus_tag="KPH93_003677"
     CDS             complement(1496..1894)
                     /locus_tag="KPH93_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690418.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLILSIIVIFFVIVLVVLIFIKRKKKKFY"
BASE COUNT          630 a          288 c          190 g          808 t
ORIGIN      
        1 tagacaacat aagaataaaa tagatataat ctactttatt cttatgttat ttagttatta
       61 taatggttta taaagtaaaa tattatttcg tatttaagct tgataactaa ctcttcttat
      121 tgatagacta acaatcttga ataaaaatat tcctgcaact agatttatta aaacagctgg
      181 taatactact gctaagaata aagctgtgaa tgaagcccct ggaagaccaa ctatagcttg
      241 agctgctaat aaaaatatag ttccacttac aagagttcct attggcatta ttattgcaac
      301 tactacagta tcttgtgttt tcttagctaa tttttttatg aaaggcatct tataactaat
      361 gtgcattaat aaaaatacta tatttgctgt aagagtttta tctattacat taggaatttg
      421 acctcctgga aaacttgatg taagtgctgt aaatattcct gttactatac ctgctattaa
      481 acaagtttta taatcatctc tatttaaaac caatattaca aataacattg ctagtggtac
      541 atctggcttc attggtaatc ctatagctgg tgtgatttga tgtattaata atcccatcgc
      601 taaaagtatt gcatttaaag ttaatttttt tgtatttgtt ttcatttttt gtacttcctc
      661 ctattttttt aattttcaat ttattttttt gccaacctac tatatcatca tctttagctt
      721 ttgaaattca taacatcttt ctcaccacct tctatctttt aaattttcag taaaataaaa
      781 aaactccgtc ctaaactaat aggacggaga ttatccgcgt tgccacctaa attatataaa
      841 aattaatata aaattcaatt ttcatacatc tcattatcag gtacttacat accttattcg
      901 ctataacgtg cgactgacga taaagtctac tctcaaaact atgatttcaa tttattcctc
      961 taagggccat tcatcaaatt ttttcttgct aagattccac catcctcagc tctctatact
     1021 gaacctaaat ttaactactc atccttgtca taggatttaa tttattgtat tgaattttgt
     1081 tattaaaata atgatattct tttttctaaa aaatgtcaac acctttttat aaaaaatata
     1141 ttttgtaatc tattttttat aaaaaaagta aattatacta tacatttctt ttcatataca
     1201 aaattctata aataattact ttaatgctct aatagtctgc tttttggctc taattcttct
     1261 tctattaatt ttgtgaatat ctgttgacgt aattttatat tttgatgcat gaggaattaa
     1321 attctttaaa gttaattaag tacatttatc aataaattta cttaatttgc catcatttaa
     1381 tactcatttc tgtatttata tacctccttt gaaacagaat attactccgt tttaattaca
     1441 tcatattatt aaatagatac caatgattag aaaaatgata ttcttttaat tatttttagt
     1501 aaaatttttt cttctttctt ttaataaata ttagtactac taaaactata acaaaaaata
     1561 tgactattat ggacaatatt aattttgtta ggatattatg ccttcttgca tttcgtcttt
     1621 tatatttatt tatagtttga tatccatact ttttagttct tatacttcgt tttttcaact
     1681 ttactttact tttattgtat ttcctacttt tatgtctact tttagattta atagttttaa
     1741 atttagtatt atcatatgta taattctctt ttattgaaga aatgtataca ctagactcaa
     1801 taatagcttc tgcttctact tgtaatggat ttatgatgct gattataagt agaaaagata
     1861 tagctatccc aatcaatttt ttcttcttat tcatattttc cccctcataa aaattt
//
LOCUS       DAEMAE010000364         1899 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEMAE010000364 DAEMAE010000000
VERSION     DAEMAE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.371"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..728
                     /locus_tag="KPH93_003678"
     CDS             <1..728
                     /locus_tag="KPH93_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1690419.1"
                     /translation="ILRPVRDINGYRMYNISDIWKLNLIKEFRSLNFPMKKIKEYLDD
                     RSIESTKNILNEELDLIDKKIAEFISHKENIIKRLSSIESVIQNTKIDEIEVVYIEKR
                     KALELNADIKRDEDFDFLIQKLQKEYEDRFNILGNNNIGSAFSTEAINKGIFNEFKSV
                     FCFLESNEKVYNIVFDEGYYVTLNYTGSNSNNKMYMEKVFKFIEENNYKIIADPIEIY
                     KIDIHETGIVEEFVTEIQVPITK"
     gene            881..1120
                     /locus_tag="KPH93_003679"
     CDS             881..1120
                     /locus_tag="KPH93_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690420.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYVGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDEK"
     gene            complement(1307..1483)
                     /locus_tag="KPH93_003680"
     CDS             complement(1307..1483)
                     /locus_tag="KPH93_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690421.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1537..1633)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          785 a          185 c          295 g          634 t
ORIGIN      
        1 gcattctaag accagttaga gatataaatg gatatagaat gtataatata tcagatatat
       61 ggaagttaaa tttaataaaa gaatttagaa gcttgaactt tccaatgaag aaaataaaag
      121 aatacctaga tgacagaagt atagagtcta cgaaaaatat tttaaatgaa gaacttgatt
      181 taattgataa aaaaatagct gaatttataa gccataagga aaatattata aaaagattga
      241 gttcgattga aagtgtaatt caaaatacaa aaatagatga gatagaggtt gtatatatag
      301 aaaaaagaaa agctttagaa ttaaatgctg atataaagag ggatgaagac tttgattttt
      361 taattcaaaa acttcagaaa gaatatgaag atagatttaa tatacttgga aacaacaata
      421 ttggttctgc cttttctact gaagcaatta ataaaggtat atttaatgag tttaagtctg
      481 tattttgttt tctggaaagt aatgagaaag tatataatat agtttttgat gaaggatatt
      541 atgtaacttt aaattacact gggagtaatt ctaataataa gatgtatatg gagaaggtgt
      601 ttaaatttat agaagaaaac aattataaga taattgctga ccctatagaa atctacaaga
      661 tagatataca tgagactgga attgtagagg agtttgtaac agaaattcaa gttcctataa
      721 ctaaatagct atatttcaat ggattagatg gtaaatagaa tgaaaaaatg ttgatttgcc
      781 accgatttgc cactgtgtta taaaatcggt agcaaattat attttaaatt ataagatagc
      841 atgataacat gaaaaaataa ttgtttaggg agttataaat gtgaaatgtt ctaattgtgg
      901 aagtgtaaat attgaaaagg gaattttaac aactggtggc atatatgctg aaactattgg
      961 attaaaatat gtaggagatg gaaaattaga taaagttata aataaaactt ttccagtaca
     1021 atctactata tattcagatt tatgtttaga ctgtggtgaa atagttagaa cctatataaa
     1081 ggataatact gataggaatt ggtgtaaaga tgagaaatag aatagttttt gaagtatatt
     1141 aatatttgaa gttgaatttt attaaagata caagtgaaaa agtataaatg ggaagaaatt
     1201 gttatatgga agaaactttg catataggag agagtagttt tttaatgtaa taacattttt
     1261 aaatgtagat aaaactaaaa agggttctct agaaaacaat ttctatttaa ttatacttac
     1321 ttaccaatac catcacttga tgtaaacaag attctactac attttctcca tactctagta
     1381 taaaatcatc tagctgtgta tctataaaat cacaataatc atataaacta ctgatataag
     1441 cagttaacat gtgtgtacat ttatccattt catctctaaa catatatatt actatccccc
     1501 taaaaaactt tcataaaaca aaaagaacaa tatctttttg gcaactggct gacataactc
     1561 atagtttatt ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt
     1621 gccgatttag ttttattcta caactaaaat aatacactag gataagttat tgttcaacat
     1681 acttgtcaga aaattgaaat atttaacaat atcttaacaa tatgaaatct attttacatt
     1741 taagaaacaa actatacaag tagaatactt aataaaagtt atatttagtt tttgtattaa
     1801 aaagtttagt tcacaaaggt tactttgcta gataatagat gaacaaaaat atcttgaaaa
     1861 aatatatatg aatggcaaaa aacaagggat gaattttat
//
LOCUS       DAEMAE010000365         1856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEMAE010000365 DAEMAE010000000
VERSION     DAEMAE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.372"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..773
                     /locus_tag="KPH93_003681"
     CDS             57..773
                     /locus_tag="KPH93_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690422.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKGLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSECVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGDRNLVYFFFGAELQSIDGIVIFKDLKHVKTKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            801..1739
                     /gene="blaCDD"
                     /locus_tag="KPH93_003682"
     CDS             801..1739
                     /gene="blaCDD"
                     /locus_tag="KPH93_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG1690423.1"
                     /translation="MKRKNNFIWIVILLVVVAMGIYYVGRKHNNTKQKYDKNYINSEL
                     NKSKIENNDKNKKEKINIVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLIN
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILKDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          772 a          153 c          310 g          621 t
ORIGIN      
        1 tagtaagttt taaataaaga acaaataatt acctcacaaa ctcaaaggag gacataatga
       61 tactaatagt tatattgagt atattcatca tagcctcaat caacataatt atattaggta
      121 aagaaaatat aagagatata gttatccctt taactctatt tattgtaagt gattttttat
      181 ttttgtgtgg aaatataata tgtaggggag gagtaatgct actatttata ggtttatttg
      241 caataccatt gatgctaatt gcatttatat ggatttgtgt acatatgatt aagctttact
      301 ataaaaaagg tttaaataaa agaaaaatga ttggatgtat atttagtatc tttataagta
      361 tagggactat atttattcct agtccaagtg aaagttttag atttaaactt tatgaaaaag
      421 attatagtgt agtagctaaa gctattttga aagtttatga tgaaaaaaag ttaagctctg
      481 aatgtgtctt ttatattcaa tctgataaaa atgaattaga gaagatattt tctaaagaag
      541 taataaaaaa aatgaaaaaa ctaaacagaa atttagacgt agttaaaatt tctggggata
      601 gaaatttagt ctattttttc tttggagctg aattacaatc tattgatggt attgtgattt
      661 ttaaagactt aaaacatgtt aaaacaaagt tggattttat aaacagaagt attcaagctc
      721 ctagattgga gtatattaca aataatgtat attattttaa aggtgaacta taaacttatg
      781 tagaggagat ggtataaagt atgaaaagaa aaaataattt tatttggata gttattcttc
      841 tagttgttgt agctatggga atatactatg taggaagaaa gcataacaat acaaaacaaa
      901 aatatgataa gaattatata aactctgaat taaataaatc taaaattgaa aacaatgata
      961 aaaacaaaaa agaaaaaatt aatatagttg actatagtga ttgttttgaa ggtattagtg
     1021 gaggagctat tttttataat actaaaaata aagaatataa tatatataac aaagaattaa
     1081 ttgaaacaag aaggtcacca tgttctactt ttaaaatagt ttcaactcta attggtttag
     1141 aaaaaggggt aataaactca aaagaatccg ttatggggta tgatggtaca aattatccaa
     1201 ataaaaattg gaataaaaat cttagtttag aagaggcatt taaggagtct tgtgtttggt
     1261 actacaaaaa attgataaat aaagttgatg ctaaatctgt ccaaaatatt ttagatgatt
     1321 taaagtatgg gaattgcgat attagtgaat gggaaggaga tttaaaaaat ggaaagggac
     1381 atttaaatgg attttggtta gaatcttcat tacaaatatc tccaaaagaa caagtgcaaa
     1441 caatggcaaa aatatttgag ggagatacaa actttaaaaa agagcatatt aatattttaa
     1501 aagatattat gaaaatagat gtaaatgata aaaacataaa tgtatatgga aaaactggaa
     1561 caggatttga tgaaaaaaat aaacgtgtgg atgcatggtt tgttggtatg ctagagcgtg
     1621 aaggtgatac ctattatttt gctattaaat cagatgattc caataaagaa attacagggc
     1681 caaaggtaaa ggaaattgca attaatataa ttaagaaata ttatagtgta agggaataaa
     1741 cttaatttta tttgtaaata ttggttacct atagtctgta atatttatta aataatttta
     1801 ctaaattgag tctatataga gcaaaactcc tttagtacat aaaatacttg ctattg
//
LOCUS       DAEMAE010000366         1840 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEMAE010000366 DAEMAE010000000
VERSION     DAEMAE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.373"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..1671
                     /locus_tag="KPH93_003683"
     CDS             67..1671
                     /locus_tag="KPH93_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1690424.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLEQLGV
                     TNETQDPEGGHIGRAEGSKEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLR
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYVNR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT          779 a          200 c          325 g          536 t
ORIGIN      
        1 gataatataa ttaagtagaa aataatgaaa aaaatttatt aaagtagaca acgtaaggag
       61 aattttatga aagaaaaaat ttattacaat ggaaatatca tcacaatgga agattcaata
      121 tgtggagatg ctattcttat aaaagacaaa ataataaaaa agataggaac aaaagaagaa
      181 gtatttgctt tgaaaaataa agatacagaa attatagatt tacaaggtaa gacattgatg
      241 ccatctttta tagattcaca tagtcacttg atagcttttg ctactactct aaagctagtt
      301 cctttagaag atgccactag ttttaaagac atagtaaaaa aaatacagga tttcaaagag
      361 tcgaataata taaaaaaagg agactggata attggatttt catatgataa taatttttta
      421 gaagaaaata agcatccaga taagagtgta ctcgatagtg catcttcaga aaatccaata
      481 ctaatttcac atgcttctgg tcatatggga gttgcaaata cattggggtt agaacagctt
      541 ggtgttacta atgaaacaca ggacccagaa ggtggacata taggtagagc agaaggaagt
      601 aaagaaccaa atggatattt ggaagaaaat gcatttttta atgtagcgtc taaaataaaa
      661 caaccatcat caaatgagat ttttaattca attgaaaaag cacagaacat atatctaagg
      721 tatggtatta caacagctca agaaggttta atggaagaaa atcaatttaa cattttaaaa
      781 gcaatggcaa atcaaaataa attaaaaatg gatgttgtag gatatgtaaa tttgaaaaaa
      841 agtaagtctg tagcagataa taatagggag tttataaaaa aatatgtgaa tagatttaaa
      901 ataggaggct ataagatttt cttagatggt tctccacaag gtaagacagc atggttgagt
      961 agaccatatg aaaattcaga tgatggttat tgtgggtatc ctatatacaa agatgaagaa
     1021 gtagaaaagt ttatagatat ttcactaaaa gaaaagatgc aactcttaac tcattgtaat
     1081 ggagatgcag cagcagacca gttgatagat gcgtttgaga aagttttaaa cttaaaagag
     1141 caaagtagtg aaaataatat aagaccagtt atgatacatg ctcaaacagt aagagcagac
     1201 cagattgatg atatgaaagt aataaatatg ataccttcat actttgtagc tcacacatat
     1261 tattggggtg atattcatat caaaaacctt ggagaagata gagcgtttaa gattagccca
     1321 cttaaaacaa ctatagaaaa gggattaatt tacactcttc atcaggatac accagtaata
     1381 gcacctaata tgctggaaac agtatggtgc gctgtcaata gaattacaaa aaaaggtata
     1441 caaataggag aaaatgaaaa gataagccca ttagatgctt taaagggtgt tactataaat
     1501 gcagcatatc aatattttga agaagataaa aaaggttcaa ttaaagaagg taaattagct
     1561 aatttgataa tattagatga aaacccactt acaatcgacc ctatgaaaat aaaagatatt
     1621 aaagtattgc agactataag agaaggagaa gtattgtatt ctttgaagta gatgaaagtt
     1681 agatgtttta taatctataa aaaacagctt tatataataa attaggtagg attaaataag
     1741 gttacagtta aatcaacaat tatatttttt attaaatttt aattattgta ttgaagattt
     1801 ataagtataa ttttattgat tctacctttt taaataactg
//
LOCUS       DAEMAE010000367         1831 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEMAE010000367 DAEMAE010000000
VERSION     DAEMAE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.374"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..543
                     /locus_tag="KPH93_003684"
     CDS             97..543
                     /locus_tag="KPH93_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG1690425.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVNATTSVTNQ"
     gene            564..1607
                     /locus_tag="KPH93_003685"
     CDS             564..1607
                     /locus_tag="KPH93_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG1690426.1"
                     /translation="MGIKIVLSIVLIVVVIAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPHDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQIATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          764 a          191 c          333 g          543 t
ORIGIN      
        1 ttattgtttt gtaatttaaa tttatattca aaaacattgt ataaacatat aatttatata
       61 gggttttata attcatatga aaagagggta gatagtatga agttaatatg gttgatagta
      121 gcagttttat ttggaatagc agaaatgtta acaccaagct taacattgat atggtttagt
      181 gtaggagctg taatacttat atttttaagt agctttatag agagtatttt cttacaaatt
      241 ttaatatttg cagtaatttc tatagcaatg ttagtcgtag ctactaaaaa aatagtaaaa
      301 aaggataaag gctacaaatc taacactaat ttacaagcaa tgatgtctaa aaaaggtatt
      361 gttacagaag agatttctcc aaataacaca ggtctagttg tagttgaaca tgaaacatgg
      421 acggctattt ctatagatgg tgaaaagata gaaaaaggtt ctacagtaga agtattaaaa
      481 attgaagggg taaaactagt tgttaaaaaa gtaaacgcca caactagtgt gacaaatcaa
      541 tagaataaag gaggatttat agtatgggta ttaaaattgt tttaagtatt gtattaatag
      601 tagtagtcat agcaataagt ttaacctgta taagagttat aaaacaatct aaagtaggta
      661 taataatgag acttggtaag tttcaaaaag tggctgaaac aggtgtacat ttcttaatac
      721 catttttaga taaaatggca tatgtaattg accttagaga aattgtaata gattttccac
      781 cccaaccagt tataactaaa gataatgtaa ctatgcagat tgatacagtt gtatattata
      841 aagttacaga cccagttaga tatgtgtttg agatagctaa tccaatagct gctattgaaa
      901 atttaacagc tacaacacta agaaatataa ttggtgaact tgatttagat gaaacattaa
      961 catcaagaga tataataaat gtaaaaatga gaactatcct tgatgaagca acagataaat
     1021 ggggtataaa agtaaataga gtagagttaa aaaatataat gcctcctcat gatattcaag
     1081 ttgcgatgga aaaacaaatg agagcagaaa gagaaagaag agaggcaata cttcaagcag
     1141 aaggtaataa gtcagctgca atacttcaag cagagggtga aaaacaatct gcaatattaa
     1201 cagcagaagc taaaaaagaa gctatggtac gtgtagcaga aggtgaaaaa gaatctgcta
     1261 tattagtggc agaaggtgaa gctgaagcta taagacagac agctattgct aaggcacaag
     1321 gtgaagctga aatgataaaa agaactcaga tagcaacagc agaaggttta aaattagttt
     1381 tttcagcaat gaaagaagct gatatagaca ataatattct agcattaaaa tctatggaag
     1441 ctcttgaaaa aatggctgaa ggtaagtcaa caaaactggt tttaccttca gaagcagtta
     1501 atttcctagg aacattcaaa ggaataaagg aagttatgag tgatgataac aaagaagtac
     1561 ttgatataaa agaagttcta aatgataatg aatcattaaa aaaataaaag agttttgtta
     1621 tcataggaag gatatggaaa ttaaaggtcg aattaattaa atatagcaca aaaatgcacg
     1681 ttgaaaagaa gaggacttat aaatttttaa gtcttctttt ttgtttttat tagaaagata
     1741 aaattataaa aataaattaa cagaatcttt atacaattta tcctttacta atgtaaaaag
     1801 tgttatataa tattatataa aacatatagc a
//
LOCUS       DAEMAE010000368         1817 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEMAE010000368 DAEMAE010000000
VERSION     DAEMAE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.375"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            65..742
                     /locus_tag="KPH93_003686"
     CDS             65..742
                     /locus_tag="KPH93_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1690427.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            814..>1817
                     /locus_tag="KPH93_003687"
     CDS             814..>1817
                     /locus_tag="KPH93_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1690428.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     "
BASE COUNT          675 a          187 c          314 g          641 t
ORIGIN      
        1 tataacttgt aataattata aaacttgaga ttatgattaa aatacctttg aatggagtgg
       61 tgtaatgtat aatttactgg ttgtagatga tgatgttgat attttaaata taaatcaaat
      121 ttatttttct aatataggtt ttaatgtata tacatctgaa actgctaaag atgctatgga
      181 tatagtagaa agtatagttt tagactgtat aattttggat atttcattgc cagatataga
      241 tggatataaa atgtgcaatg atataaaaga aaaggttaac atacctataa tatttttatc
      301 taattatgag catgaagaag aaaaagttcg gggattttta gctggggggg atgattatat
      361 aacaaaacct tatagtttaa aagaacttga acttagaata tatgctcgta tgaggcagta
      421 caaaaatatt acatcagttc ctaatgtgtt aaagttttca gcgttagtaa taaatgtaaa
      481 ttcaagaaaa gtaacttttg atggagaaag tatagattta actacaatgg aatttgaaat
      541 attattgttt ctagctgaaa acaaagaaca agtattttcg aagatagaga tatataatca
      601 agtttggaaa atgcctgata ttggagatca acatacagtg caggttcata ttgcacagat
      661 gagaaaaaaa attaattctt taagcaggga gcatcaatac atacaaaccg tatggggaaa
      721 aggatataag tttgtaccat aagtgagata ctgtaattaa taaatagttc ttgttttatt
      781 taatactact atgaaagaga tgtggttgat gcaatgaaaa agtataatat actaaaagga
      841 actctcattg tattgatagt ctctctgttg gcaataacag gattgaatct tctgtataaa
      901 tatgataata aatatacata taagtctatt agagcaagta atggaatttt aactatagat
      961 aatgatactt tcactaaagg taaacttgta tttttgatag atgactggga gttttataat
     1021 cataaaatat ttaaaccaaa tgattttaca gagcaagata taaaaccaga atatgttttt
     1081 ataggtcaat atccagattt ttctatgaga aaagggaatc agtcaccata tgggaatgct
     1141 acttatcgta tgagaataaa aaatgaaggt aaggataagc tactaagttt agagcttcct
     1201 gaaatttttt ccgcaagcga agtatggata aatggagaaa tggtttctaa attaggagat
     1261 gtaggaacag aaaaatatag acccaaaatt aaaaattcag tagtaagttt tattgcaaag
     1321 aaagatacag aaattataat caatgtatcg aatttttcac attattatag tggtttgtat
     1381 tatccgcctg cattaggaga aacaaaggac ataagtaata tgatgtttta tcgattactt
     1441 ttttatagta taatttgctt tacaactttt gcaatagcta tattttcgtt gtctgtatgg
     1501 tttctttcaa acaaaagtaa actttactta tattttggat gtatgtgtgt tttctttgcc
     1561 atccatgtat cctatccctt tgtacatttg ttaggactgc ctttggtaag ttttacatat
     1621 gcggttgaag attcgtcata ttttatggta gttttatgta ttatttgtat aaatggaagt
     1681 atatccaaaa tagaagaaaa taaagtgtat agatttttaa ttttaccatt aggaattaca
     1741 atgagtatca tatctatagt gataccaatt attcttcttc catatgatat gtactttgtc
     1801 aacatatatg ggaaatt
//
LOCUS       DAEMAE010000369         1813 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEMAE010000369 DAEMAE010000000
VERSION     DAEMAE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.376"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..1561
                     /gene="ade"
                     /locus_tag="KPH93_003688"
     CDS             56..1561
                     /gene="ade"
                     /locus_tag="KPH93_003688"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG1690429.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGASAIDFLLESTKDIPLNVYIMMPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSARAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVSYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVSIAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITGNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT          693 a          199 c          333 g          588 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag tgtagcaaca aatatatatc
       61 acctggtttt attgatgcac atatgcatat tgaatctaca atggtaatgc caattgaatt
      121 atcaaaaaaa ttattaaaat caggtacgac aactatcata gccgacccac atgagttggt
      181 caatgtaaaa ggagctagtg caatagattt tttattagaa tcgacaaaag atataccact
      241 taatgtatat attatgatgc cttcatccgt acctgcaaca agctttgaaa ctaatggagt
      301 tggtaaattt agtgcaaaag acatggagag ttatgtaaat aatcctagaa ttttaggttt
      361 aggagaggta atgtgcttca atgatgttat aaattctgaa aatgaaatct tggataaact
      421 agagcttttt aaaaataaag ttgtagacgg acatgcacct aatataaatg gtaaatcact
      481 tcagacatat gtatgtgcag gaatagaaaa tgaccatgaa tgtataactt ttgatgaagt
      541 atatgaaaaa ttgagagcag gattaaaaat tttaataaga gaaggaagtg cagcaaaaaa
      601 tttaaagtct atagtaagtg gtatgctaaa gcataacttg ccaattgaag aatttatgtt
      661 ttgtacagat gataagcact tagatgatat agaaaaacaa ggtcacataa gatggaatat
      721 aaaatgtgcg attgatttag gtatggaacc tgttagggct ataaaagttg caacttataa
      781 ttctgcaaga gcatatgggt taaggaaaat tggagcaata ggtgcgggtt ataaggcaga
      841 tattgttgta ttgaatgatt tagacaaaat ggaagtggat agtgtatata aagatggtaa
      901 tttggtaaac gaagagatgt tttctaatta caattatgag attaaagata aagaattgct
      961 taatacagtt aagtttaagt atattaataa agaaaaaata cagttaaaag taagtgaaaa
     1021 aaattatgta atggagattg tatcttatca aattttgaca aataaggtat atgaatcttt
     1081 accatgtgca gatggatatt ttgttccaaa taaagagtat tcaaaacttt gtgttgttga
     1141 gagacataga atgactggaa atgtatcaat tgctccacta aaaggatttg gcataaaaaa
     1201 tggagcaatt gctactactg tggctcatga ttctcataat attatagttg caggggataa
     1261 tgatgatgac attctagtag ccataaatta cttaaaagaa atacaaggtg gttatgtaat
     1321 tgtatcaaat ggaaaagttc ttgcacacct aagtcttcaa gtagctggat taattagtac
     1381 ttttacagct gaggaagtac aagaaattac tggtaatatg ttagagatag caagaaaaat
     1441 gggagttcca gagtatgtag acccttttat tacactttct tttatggcac ttccagttgt
     1501 tccacaaata agacttacag atttaggtct atttgatgtt gaggaattta aatttatata
     1561 gataatataa aagtaataat aaatgaggct ttctcaaaat agagataagt ctctttttat
     1621 tgctgaaatg atgaaaatat atttgatttc atacaaaaaa gagcatctta tgaactaaaa
     1681 attttatttt tagatatcct tattttaatt aatctatcat ttaaccttat tttaagctta
     1741 gatatcttaa aaaaactatt ttcagcctat tttagcatat aaatacatga aaattatata
     1801 tttatgaaat ttt
//
LOCUS       DAEMAE010000370         1800 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEMAE010000370 DAEMAE010000000
VERSION     DAEMAE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.377"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(283..1611)
                     /locus_tag="KPH93_003689"
     CDS             complement(283..1611)
                     /locus_tag="KPH93_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1690430.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          660 a          324 c          203 g          613 t
ORIGIN      
        1 aaaagctatg tataatattt gttatataga tatcacttag tttgataaga aaacatttta
       61 aaataaagta ttttaaagca aataattttt tagatgttta attttataat ccaaaaacta
      121 aaaataaagt gaatacctta aaataaactt tttaattctt gaaacactct ttttttataa
      181 aatcaaacat atctttacca ttttaacaat aaaaaagaga cttatctcta tttgagatag
      241 tctctcttat tttaattata aagcatttgt aaactacttg gcttatcttt ttactcctct
      301 agtcatataa gcatacactg gtaagcctat agctgttatt acaagacctc ctatagatat
      361 catagtagtt ttcatacctg caaaacacaa ttgattaact acaacaaata taccacttgc
      421 tatagctatt ataggaacaa taggatataa tggaacttta tactctcttg gtatatttgg
      481 atgagtcttt ctaagtttca taacccctat aaaagttaat acatagaata tccaagtagc
      541 aaatacagct aagtcagtta ataaattaaa ttgacctgac aaagcatata tagatgcaat
      601 aacagatact aatgctattg catttgctgg aacttgagca gaatttaatt tagaaaatat
      661 accatacttt ggtaaagtct tatctgtagc taaagtatat gctacccttg aaccagttag
      721 taaaaatcca ttcaatgctc caaacactga aattaatata cctacagata ttattttccc
      781 acccattgaa ccaaatataa cttcagcaac tgctgatgca ggagaagcat aatttgctag
      841 ttcattagct ggtaataccc aaagatatgc taaatttatt ataaaataaa cagccataac
      901 aactgataat ccacctatta tagcttttgg aaggtctttt ccaggattct tcatttctcc
      961 agctaaagta ccaacattca tccatccatc aaaagcaaat aatatagcaa ttaaaacttg
     1021 acctagtaca ctacccagac ttaatccttc accaaccata ggtgtaagta ttggattatt
     1081 tccaccacct ttaataaatc caacaaccat tattaaaata agtggtatta acttacaaat
     1141 tgtagaaaca gtttgtatga ctcctccagt ttttgaacct aacatattaa gccctgcaac
     1201 tattactata attccaattg ctattggaag taaaagtgat ggactaccaa ttagtgctga
     1261 tgcttgttct ccaaatataa cacctaatgc tgccatcatc cctggataaa ataatacaat
     1321 ttgcatccat ccagttaaaa aacctaattt ttctccataa atttctttta tatataccat
     1381 cattccacca gtttttggta tcgcaactga tacctctgca gctgtaagac cagctgttat
     1441 agttatgatt ccagctatta accatgccaa tataccaaga cctggagctc ctccagttaa
     1501 tgtatagact gcttgtggtt taaaaaatac gcccgctcct ataacggaac caacaacagt
     1561 tgataatgct gctgatacgc caagagtctt tttcagttca ttacttccca tttttttcct
     1621 cctcattaat cctatttaat tttaatttca tagttaaata agcatatatt atgccaatat
     1681 atagaaaaat agttttatta tttattaaaa taaaataaac tttatttagc ttttgtaatc
     1741 ttttactttt agttatgttt aaatataaaa aaattataaa aaacaaaaat ccaaatataa
//
LOCUS       DAEMAE010000371         1781 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEMAE010000371 DAEMAE010000000
VERSION     DAEMAE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.378"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..867
                     /locus_tag="KPH93_003690"
     CDS             <1..867
                     /locus_tag="KPH93_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1690431.1"
                     /translation="NSIGVIRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQIS
                     KNIINFCSFNDIRKDLQEKEKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTL
                     NSMASIALDDGSYDLYSAIIDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYG
                     DALKVKYLIPEKLYNLSVPFNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIE
                     IHNNGTILDGNNIDKIKAGIRSNNGHGLALIYTKFTSAYGNNFDMDIKSSDNEGTYIM
                     IKIPIENYKEYV"
     gene            981..1679
                     /locus_tag="KPH93_003691"
     CDS             981..1679
                     /locus_tag="KPH93_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG1690432.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIIGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFDYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNKILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKME
                     KGMTPKVYKLNTKN"
BASE COUNT          747 a          173 c          267 g          594 t
ORIGIN      
        1 aatagcatag gtgttataag aggagggtat gtactattat ctgatttaaa tttagataca
       61 gagcataata atttatatga tattccagag ggagctgcta gaagtataaa acgcctcttg
      121 aaacaaatat ctaaaaatat aataaatttt tgctccttta atgatataag aaaggattta
      181 caagaaaaag agaagactat agcaagaaca tatcattatg gagagcaact tgaaatgaat
      241 ttaaaggtag ctcaagatat ggttactaat ttaagaataa atcatcactt tttatttaat
      301 acattaaatt caatggcaag catagcacta gatgatggta gttatgattt gtatagtgca
      361 ataatagatt tatcaagaat gtttagatat acaatgaggt ctgatttaag atttgtagaa
      421 ttagaatctg aaatattata cattaaaaat tatttgaatt tacagaaatt acgttatgga
      481 gatgcactta aagtaaaata tcttattcca gagaagttat ataacctttc tgtacctttt
      541 aactttattc aacctattgt agagaatgca tttacacatg gatttagaga tattgacaca
      601 gaaaaacgaa ttgaaataat tgcaagatta gattctcagt atgctattat agagattcat
      661 aataatggaa ctattttaga tggaaataat atagacaaaa taaaggcagg cattcgtagt
      721 aataatggtc atggtttggc tttaatatat acaaaattca catctgctta tggaaataat
      781 tttgatatgg atataaagtc gagtgataat gaaggaactt acataatgat taaaattcct
      841 attgaaaatt ataaggagta tgtatagaac ttatgaaaaa ttaataagtt ttataaatgt
      901 taagaaatat attagatgtt tttatgttgt tttatatatc ttactttttt atattatcaa
      961 gctaaaaggg aggtattctc atgagaagag cactaataat agatgatgaa gaagctgttc
     1021 taaaaataat aacacatttt atagaaatga aagatatgcc aataaaaatt attggtaaag
     1081 caacatctgg tgatgaagca gtagataaaa tcattggttt agagccagac atagttttta
     1141 tagatattca aatgccaata tacaacgggt tagaagtaat agaaaaaaca tctcatttat
     1201 ctaaaaaaat aaactttgta gtaataactg catttgacta ttttgaatat gctcaaaaag
     1261 ctttgagatt aaatgtaaaa gatatactcc ttaaaccatt ggacatgaaa gaatttacta
     1321 agtctgtaga aaaaattatt ggatatcaat ataccaataa tgatttatta aataaaatac
     1381 tggaatatat aaatctaaat tatagcaatg acttaaagtt aaatgattgt gctagattat
     1441 ttttaaccaa tccatctaat atatctagga tttttaaaag taatttgaat acaactttta
     1501 tatcatatct aaatcatata agaatagaaa aatctattga gttacttgaa aatactacaa
     1561 aaagtataaa tgagattgca gaaatagttg gatataatag cctaaataac ttttataaaa
     1621 actttaagat ggaaaaagga atgactccaa aagtttataa actgaatact aaaaattgaa
     1681 attgataaat aaaaagtcaa tatagataat tgtttgagtg tgtctgaata agtatacttt
     1741 aattatgata aaaattaaag cgctttaaac atatttaata a
//
LOCUS       DAEMAE010000372         1778 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEMAE010000372 DAEMAE010000000
VERSION     DAEMAE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.379"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..1742
                     /locus_tag="KPH93_003692"
     CDS             162..1742
                     /locus_tag="KPH93_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG1690433.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNIEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDIAGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEEGL
                     NKLLNSSDAIGFGPGMGNNSQTFGKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NKFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNVVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          691 a          144 c          354 g          589 t
ORIGIN      
        1 taaataattg ttttaattca aaattttttg ttattttaaa ttatttcaaa aatcttagtt
       61 ttagtgattt tatttatgat atttatggga ttagaaagat ttcaatagat attttttggt
      121 aagtatttaa gaagtcttta actttatagg agtgataatt tatgctaata ggtacatcta
      181 aattaagcag aaatgttgat actgcatgtg tagaagaagt agggattcca atgattgtat
      241 tgatggaaaa tgcagttata tctgcaatga aaaatatgga tatagacatg tataatagtt
      301 atacagttgt atgtggtgtt ggaaataatg gaggagatgg tcttggaata gcaagacatc
      361 taaatacata tggaaaagaa gtaaacgtat ttttagtagg taatattgaa aaactaagtg
      421 aatgttctaa aataaattac aatgtacttt taaatatggg aattaatatt ataaatatta
      481 ataaaaatga tttaagtgaa aaaaattatg gagatgaaaa tgaagtagat aaaataatta
      541 aagttgagaa acaaaatatt ataaaaaata aaaatttaac aatttttaaa gatagagtat
      601 ctaaaagtga tgttgtagta gatgctatat ttgggactgg actaaaacgt gatatagctg
      661 gtatttttaa agaagttatt gacatagtaa acgataatag caaaaataca tattctatag
      721 atattccttc aggtatagat agcgataatg gaaatgtatt aggaacttgt attagagcaa
      781 ataaaacgat tagctttgag ttttataaaa gaggttttct aaactatgat actagcgcac
      841 ttataggtga tgtagttgta gaaaagatag gtgttccaga ttttattaca agaaaatacc
      901 atgatgaaga gttcataacg gataagactt ttgtaaaaaa taatattaga agaagagata
      961 agcatggttt taaaagtgat tttggaaaag tatctatagt tgcaggttca aaaggatttt
     1021 atggtgcaag tttcatagca actgaatctg ctgttaagag tggaagtgga cttgtaacat
     1081 taattagtag tgaagatgtg caagaaaagg tatctattag acttacagaa gcaatgactt
     1141 taaattttga agaagaagga ttaaataaat tacttaattc tagtgatgca attggttttg
     1201 gtccaggtat gggaaataat agtcaaacat ttggtaaatt acttaagata gtagaaaatt
     1261 ctaattgccc aatagtatta gatgctgatg gattgaacgt tatgaaggac agatgttata
     1321 agtttttaga atggaaaaat aaatttgtaa ttacacctca tttgggagaa atggccaggt
     1381 tgacaggcga ttcaattggg tatataagag aacatagagt agatgttgcg aaagaatttg
     1441 ctcaaaaata taatgtggtg gtgcttttaa aaggttatca aactgtcata acagatggta
     1501 aaaaaacata tataaatcct acaggaaata gctgtatggc aactggtgga atgggtgact
     1561 gcttgcttgg aatgattact tcatttatag gtcaaggtat ggatatattg gaagctacag
     1621 ttagtggagc atatatacat ggctatatag gagataaatt gagcaaagca atgtatacgg
     1681 taaatgctac ggatttaata agtaacattt cattaacaat gaaagagttg ttggatatat
     1741 aattggtaat atttgatact actttgtaac aattttaa
//
LOCUS       DAEMAE010000373         1772 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEMAE010000373 DAEMAE010000000
VERSION     DAEMAE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.380"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..979)
                     /locus_tag="KPH93_003693"
     CDS             complement(<1..979)
                     /locus_tag="KPH93_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690434.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLLKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKSPEKYRFKKFIKLKCALGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSAMYMIIDAVKKRKSFYYKGQNLIAFSEFGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYV"
     gene            complement(966..1733)
                     /locus_tag="KPH93_003694"
     CDS             complement(966..1733)
                     /locus_tag="KPH93_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690435.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESSREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT          635 a          300 c          154 g          683 t
ORIGIN      
        1 ttacataaga atttcctaaa tttatagtat aactgtattt ataattttca cttatattcc
       61 tatctatatc atagtaaagt gatatggtaa atcctaacat agttacactt gtagctatta
      121 atattgctat tacagccaat acactgctat tgctctttaa tttaaatcca aattcagaaa
      181 atgcaattaa attctgtccc ttataataaa aactttttct cttctttaca gcatcaataa
      241 tcatatacat agctgatgaa aaaaataaaa aagttccagg tattattgta actaatataa
      301 aaactgaaag cataatattt tcagcaataa aataactaat tgacattaaa taagaaatag
      361 ctattaataa aacaccaagt gcacatttta actttataaa ttttttaaac ctatactttt
      421 ctggagattt attaaataac tctataattc tcttatttct gataactata gtatttctta
      481 ttccaataac tataaatatt attaaaaata ccgctagtgt ctgaactaag gcctttatac
      541 ttaagttcat atttactact atcatttcat tcattaattt taaaagcatc atagtcataa
      601 gctttgaaaa taacagtcct aaaaatatac ctattagtat tgcaactata cccatcaaaa
      661 aagtttcata taaaaatatt tttcctatct gatttttcct cattccaagt aaactataag
      721 ttcctacttc tctcctacgt ctattaaaaa aaaatgagtt tgaataatat ataaataaaa
      781 atacaaatac tactattaca attgaacttg tattaatact catcctagtt ttctctccaa
      841 gtgaactcaa tgcttcatta tactctatag acttaaaact aaaaaataca aatacactaa
      901 atacaattga aataaaatac attaaataat taaaaaaact attctttaca ttttgcattg
      961 caattctaaa taaagtcatt tttattaact cctccaatta aggaaactgt atttactatt
     1021 ttattataga actctctgct actttctccc tttacaattt ctgtgaatat tattccatcc
     1081 tttataaaga gtattctatt gcaaaaactc gcacttatag catcatgagt taccatcaat
     1141 attgttttat tgctatcttt gtttaatttt aataaacaat tcaacaaatc cctagcagat
     1201 ttagaatcta atgctcctgt aggctcatct gcaagtacta atgatggttt tgttgctatg
     1261 gctctacata cagctcctct ttgcttttgt ccaccagaaa cttcgccagg gtatttatct
     1321 aatatagatt ctatatttaa ttcttttgaa atctctttta ttctattttc tattactgat
     1381 actctttcat tttttaaaga aagaggcaac attatatttt cttttattgt aagtgtatcc
     1441 aaaagattag aatcttgaaa tacaaagcct agattatcac gtctaaaatc tgccaattca
     1501 ttttgcttca ttttaatagt attctttcca tttatataaa tatctcctga tgaaggtaaa
     1561 tctattgtag aaataatatt taaaagtgtt gatttaccag caccagaaga ccccataata
     1621 ccaacaaatt ctcctttata gaccttgaaa gttattcctc ttagagcttc atgttttgaa
     1681 ccttttcttc catactcctt ttttatattt tcaacagata atatttcttc catagttaat
     1741 aatcaccttt catcataatt agttatttat ca
//
LOCUS       DAEMAE010000374         1771 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEMAE010000374 DAEMAE010000000
VERSION     DAEMAE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.381"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            81..827
                     /locus_tag="KPH93_003695"
     CDS             81..827
                     /locus_tag="KPH93_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG1690436.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITRLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLEGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNE"
     gene            820..1701
                     /locus_tag="KPH93_003696"
     CDS             820..1701
                     /locus_tag="KPH93_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG1690437.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGLHEDRLLEYPIMKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITKENNNITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT          737 a          207 c          263 g          564 t
ORIGIN      
        1 gacatttttt cccttagatt ataaaattaa attaataact acataggtag ttactaatct
       61 ttcatcaaag gagtttctct atggataata taattaatga attacaaaat ataggattta
      121 ctaaatacga atctcaaata tacatatcat tattaaagga aagcccttta acaggatatg
      181 aaataagtaa attatcaggt gtaccacaat caaaagtcta tgaaaatata acaaggctat
      241 taaacaataa tataattatc aatgttggaa ctgaccctat taagtacata cccataaacc
      301 ctaatgaatt attaaaaaat aagaaaaaag aatttgataa ttctatggat aatctaaaga
      361 atagcttaca gatattaaat aaaaataaaa gtgtagaata cgttttaaac ataaaaggag
      421 ataaaaatat attaaaaaaa gcattagaaa tgataaaaaa tgcaaaacaa gaaattataa
      481 tttcttgccc ttatgaagaa attcttgaat taaaaaaaga actcctaatt gcttacaaaa
      541 aaaatattaa tattgaaata ctacttttag aaaacaaaag tctagaaggt cttgataata
      601 ttcatattca tggaggtaat aaaactacac ttgaaaataa aatgctttac aaaggaatta
      661 ttttgattgt agataatgat gaaatattga caggaaactt atctgaaggc agtgaagcca
      721 tttcaatttg gacaaaaaac tctaacattt tatatattgg agttcaatac ataaaacatg
      781 aaatttatat ttcagaacaa ataaaaggag gttataaaaa tgaataaaat taagattaaa
      841 caagttgatg cttttacaac tatacctttt ggtggaaatc ctgctggtgt cgttactgat
      901 gcctctaata tttctgatga aattaagcag aatgtggcta aggaaatgaa tttatcagaa
      961 actgcatttg tttcagagtc aaatgttgca gactttaagg tacaattttt cacaccaggt
     1021 ttagaagttg atttatgcgg tcatgcaaca ataggtactt tccatgcact tttggaagaa
     1081 ggaaagctta atactaataa gagtatattt tatcaagaaa caaaagctgg tgtattatct
     1141 gtagagctta aggaaataaa taatgaaaat atatttatga tggaacagaa tacacctgaa
     1201 tttgaaaatt tagataatta cagaaaagaa cttgcacaaa tgataggttt gcatgaagat
     1261 agacttcttg aatatcctat tatgaaggta aatactggac tttggtggct tgtatttggt
     1321 ctaaaatctt tagatgattt aaaaaatatc tctccagatt taaataaaat taaaaaattt
     1381 agtggtgata ataatataat tggcataact ccattttgta tagaaactat agataaaaag
     1441 tgtgactacc atttaagatc tatagcacca tatgttggag ttacagaaga ccctgtatgt
     1501 ggaactggga acgcttgcgt atcatcttat atagtacatc acaatatttt agataaaact
     1561 agttttattg gtgagcaagg taactttata aacagacctg gaaaagtata tgtaaatata
     1621 actaaagaaa acaataacat aacttctgta aaaattggag gaagtgctta tactgtttta
     1681 agtggtgaaa ttatgtatta atcccctacc taataaaaac aaataaaaat actagctaac
     1741 taaggctagt atttttattt gttattttta t
//
LOCUS       DAEMAE010000375         1743 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEMAE010000375 DAEMAE010000000
VERSION     DAEMAE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.382"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(111..1289)
                     /locus_tag="KPH93_003697"
     CDS             complement(111..1289)
                     /locus_tag="KPH93_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1690438.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGDKNITIHKHYKYFFL
                     IKPLTFLIIIFTKLPLPLYYILFYILNHKLLLSRKSSTNNHFIPNIAVISFCILHLIT
                     VSTMSLVKNTSPDIVSVNMSYKLISILVASILDFILWYYMDKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDDLTSAYNRKFAFEKIQHYIDNSLLFSLI
                     YIDLDKLKTINDTYGHLSGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVPSESKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT          634 a          184 c          216 g          709 t
ORIGIN      
        1 ttttagagtt attaatcatt atgtctaaat atttaaaaaa attataatga tagagataat
       61 aagataaaaa acatagtaag attacaagta agctcatgta aatattcatt ttagttattg
      121 ttccttttat ggttattttg tttattagtg tacattctaa tatcagcaat attaataatt
      181 tcttctacag tcattttgct ttcgctagga acttccacaa taccatagct aaaagatatc
      241 ttattatcca taattttact tattttctct ataaattctt ttgtagtgtt ttcattagat
      301 ttaggcataa taactataaa ctcatctcct cctattctag caaatatgtc atctattcct
      361 aagttttcaa taactatact tgagaagtta ataatatagt tatccccaga taaatgtcca
      421 tatgtatcat ttatcgtctt taatttatcc aaatcgatat atatcaatga aaataataaa
      481 ctattatcaa tgtaatgctg tattttttca aaagcaaatt ttcgattata tgctgatgtt
      541 aaatcatcaa tgttagcttt tttctttaat aaattgactt ttagtaattg ttcttgcttt
      601 atcattttta gtctattatg ttctttttct aaataaatat ttttttcaat ggaatatata
      661 tgattgatga atataattag ttgtaagatt aataatatgt tacttcccaa tataaagtag
      721 gaagttagag aacttggtgg agcaaatgta atttgtatac tatcaaataa tatatatcct
      781 aaagtgaacc agataaactt caaaaataga ctgaactcta tttgattttg agttattata
      841 taaatatgtt cttttatttt atccatatag taccataaga taaaatctaa aatagaagct
      901 actaaaatac taattaactt ataggacata tttacagata ctatatctgg acttgtattt
      961 ttaacaagtg acattgtact cacagtaata aggtgtaaaa tacaaaaact tattactgct
     1021 atattgggaa taaaatggtt gttagtagaa ctttttctgc ttaagagcaa tttatgattt
     1081 agtatgtaaa acaatatata atatagtggt aatggtaatt ttgtgaatat tattatcaag
     1141 aaggttaatg gttttattaa aaaaaaatat ttgtaatgct tatgtatagt tatatttttg
     1201 tctccaaaag atgctatttt gcatatgtaa atgttacata gttcgattat taatatagta
     1261 gttatgatta aaaatatatg gtatttcata gtacatcctc ttttaaagtg aaattgtatt
     1321 tacttaaaat agattagatt ttatatattc tattttacag tttttattta ggtacatgca
     1381 aagagcttat tatggatacc ttctttttta ttgcatgtgt ctgtgttaga taaaatttaa
     1441 aaagtattct atcttacata tacagtatgt atgataacta tgtgtccaag tatagtaatt
     1501 atatatagta tgtatgataa ctatgtgtcc aagtatagta attacatata gcaaatataa
     1561 tagttatgtc tccaattata acaattacat atagtatgta tgataactat gtgtccaagt
     1621 atagtataat tacatatagt aaatataata gttatgtgtc taagtatagt aattacatat
     1681 agcatgtatg ataactatgt gtctaagtat agtaattata tatagcaagt ataatagtca
     1741 tgt
//
LOCUS       DAEMAE010000376         1730 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEMAE010000376 DAEMAE010000000
VERSION     DAEMAE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.383"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            184..1587
                     /locus_tag="KPH93_003698"
     CDS             184..1587
                     /locus_tag="KPH93_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1690439.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT          609 a          204 c          290 g          627 t
ORIGIN      
        1 gtaaaacttt taaatttatc atttttaata agatttgatt tcaaagtatt taaggtttat
       61 tattaaaact gtttttgaac atacaaatat tttgaaaaat atcagtttaa taataatgaa
      121 tctattttat taattttgaa attaaattta aaataattaa tagagagggg aaattaaatt
      181 aggatggaaa agaaaaaaat aggcctatgg agtttggtat ttatgaatgt atcagcttta
      241 tatggtataa gatggatagc taagtctaca gcatcaagct ttggtttagg attaggtgca
      301 atacctatgt ggttattatt ctcatttata tactttgtac caggagcttt gatttgtgct
      361 gaattagctt ctacatatca aaaaggtgat ggaggccttt acgattgggt taaggaagcc
      421 tttggagaaa agtatggatt tttagtttct tggttaaact ggacagctaa aatattttgg
      481 tattcttcat ttttaacttt cttagcagta aatgtttctt atacaatagg aaatccagct
      541 cttgctgata ataagatgtt tgtacttata ttatctttaa taatattctg ggcattatct
      601 cttatagcaa ctaaaggaat aagttttaca aaaatattta ctaatacagg tgcattaggt
      661 tcaacaatac caagtgctat acttataata ttttcatttg tagcagtatt tgtgttgaaa
      721 aaacatgata tagcttctac atacacaata gcttcaatga ctccaaaact taatatggat
      781 gcatttgttg ctatatcagc tataatgttt ggtttagcag gagcagagac tgctgctaac
      841 ttcataacag aaatagataa gcctgaaaag aacttcccta aggcgatatt aatatcagct
      901 ggaatagtag cttcattata tgtattaggt tcaattgcaa taacaatgat aataccacca
      961 gaccaaatta ctgcttctaa aggtgtatta gatgcacttt ctgctgtttg tgcgtcttta
     1021 ggaattggtt cttggttggt tcaattaata gcttttggta tagctttttc agtattagga
     1081 gcaatagttc tttacatagc atcaccaata aaaatgttat ttggtagtgt taaaaaagga
     1141 atatttccag atagtttggt agaagtaaat gaacacaaga taccttctaa agcagttata
     1201 cttcaagcaa taatagttac tataatacta ttggtgacta cattaatgcc ttctgttgat
     1261 gctatataca atgttcttgt aactatgaca gcattaactg ctttattccc atatgtatta
     1321 ttatatgctt cttatattaa actaagaaaa gagagaccag atgaaattag accttatact
     1381 atggcaaaat caacaagtac ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta
     1441 gttggtatat tattatctgc agcaccagtt atgcctacat ttgctgaaaa tgttatatat
     1501 gagatagaga tgattggtgg aggtcttcta gttatattat ctggtctatg gttatggaat
     1561 agatatgaaa agaaaacagc taaataaaat gtattttaaa aatttctttt ggatattata
     1621 atatatagtt gacaatttat agtgtaagga atattataaa ataaagaaat taataaatta
     1681 aatcctgaga taagaaatag taaataaata tatttttgca cagagaatta
//
LOCUS       DAEMAE010000377         1697 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEMAE010000377 DAEMAE010000000
VERSION     DAEMAE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.384"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..748
                     /locus_tag="KPH93_003699"
     CDS             119..748
                     /locus_tag="KPH93_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1690440.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSTLPIDKQIIEIMTSFIKT
                     LSSEPEMSKAIMHSRMNIIFASENINNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            723..1127
                     /locus_tag="KPH93_003700"
     CDS             723..1127
                     /locus_tag="KPH93_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG1690441.1"
                     /translation="MKIHNKATNKKKKYVKISIALFFVFICVIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNKLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1130..1606
                     /locus_tag="KPH93_003701"
     CDS             1130..1606
                     /locus_tag="KPH93_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG1690442.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT          711 a          189 c          232 g          565 t
ORIGIN      
        1 tattagccat aaaaatatat taacttatat tatttaataa tttattgaca gtattaaaat
       61 taaattatac aataacagtg taaattaact taacaccgtt aagcaaagag gtgattatat
      121 gagtatcaaa gaaagacgta aaaatgaaaa agaagaaatg aagaaaaaga ttatggatgc
      181 tagcatcgaa attataaacc aacatggata tgaaaattta tcaataagaa aaattgctac
      241 aaaaatagaa tattctccaa ctacaattta tctttactac aaagacaaag cagagattat
      301 cagcgatatg accaataaac tttataacac agtagaaagt aatgctattg atattatgaa
      361 taattgctca acgctaccaa tagataaaca aatcatagaa attatgacta gctttataaa
      421 aactctttct agtgaaccag agatgtcaaa agctatcatg catagtagaa tgaatattat
      481 atttgcatct gaaaatataa acaatacgcc tagcaataat ggtataaaaa tgcttgataa
      541 attcctttcc attggaattg aacaaggaat attcaaaaaa aatatagata aaagctcatg
      601 gatgcttata agtgcactgt taggattcgt tttatgtgtt gttgaaaata aactatattc
      661 tttacataat ttttctcaac ttatagaaaa tttcttagat attttagtag gaggacttta
      721 caatgaaaat tcacaacaaa gctactaata aaaagaaaaa atatgtaaaa atatctattg
      781 ctctattctt tgtatttata tgcgttatta ttgtaattgt aagcaactta aagccagaaa
      841 acttagtagt aaaagatata gatattaata atgttaaaaa tggcatttat actggaagtg
      901 ctgataataa attggtaaag gctactgtgt ctgttgaagt taataatggt aaaatccaaa
      961 atattaatat cttaaaacac gaccatcttt taggtaaacc tgctgaaaag attacaacaa
     1021 gtattattaa acaacagtct ttagatgtgg atgctataac ttctgcaact tatagcagta
     1081 atactatacg aaaagctgta gaaaatgctt taagaaaggg agaataatta tgaatactat
     1141 tattatatat tcaagtaaat atggttgtac aaaagactgt gcaaatatac taaaaaataa
     1201 actatcagat aatgtcacta ttgttgatat taataataat aataataaaa ttgagttatc
     1261 aaaatttgat aaaattatta ttggaagctc tatatatgtt ggttcagttt caaaaaaaat
     1321 acaagtgcta tgtaatgata atgtagagtt gttaaacaaa aaacaagttg gtattttcct
     1381 ttgctgtggc ttttctgaac aagcagataa atatttaaaa tcaaattttc catcatcctt
     1441 attagaaagt gcaaatgcga ttggaatttt tggtagtgaa gctcgattag aaaaaatgaa
     1501 atttttagat aaacttatta tgaaggctgt ttcaaaaggt aattatgaca gttttagaat
     1561 atcacaggat aatatagata attttttaat aaatcttaat agctaaaata aaactaatta
     1621 tctctatgat aaattctttt atttataagt aatattgctc aaaatagtct ttaaacttta
     1681 ttacaatttt atttttc
//
LOCUS       DAEMAE010000378         1697 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEMAE010000378 DAEMAE010000000
VERSION     DAEMAE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.385"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            81..338
                     /locus_tag="KPH93_003702"
                     /pseudo
     CDS             81..338
                     /locus_tag="KPH93_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
     gene            complement(640..1419)
                     /locus_tag="KPH93_003703"
     CDS             complement(640..1419)
                     /locus_tag="KPH93_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG1690443.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLIYIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          604 a          247 c          196 g          650 t
ORIGIN      
        1 tggagaatta gtaagacata ttttagtatg caaaattcta tgaagcagga accctgtgac
       61 tttagtcatg ggaggttcag gctgatgttt taataataag tctagtttct gatgatggta
      121 tattaaatga tggtacaaaa cttaaagaca caccatttaa agatgaaatc tttactttag
      181 atgagattgt tgaaattaaa aactattata gaattaaaaa gattatcgtt acacatattg
      241 acgaaatgtg gggaaaatca tatggttatt acagtgaatt agaaaagaaa cttgataata
      301 tttattttgc atatgatggt atggaaatta tagtctaaat cattaagtaa taagcataat
      361 gtaaaataaa aaaacttcaa tttaacagta tagtcaaaat atgtaatatc aaaatatcac
      421 agtttttaca aaatatcata gtatttatct ttataaaatt tcaaactcta catatttcaa
      481 tacctcaata aagtaaaatt aaaaatatat tttatataca ttatagacgg cttatcggca
      541 ctagataagc cgttttgtca caattattat ttactcaaca aaaattctca taatttataa
      601 cagattgaat ctctaactga tacttaatta caaaaactct cattccttaa tatgttgttg
      661 cattaaccaa accttcatca tcattttttg tggcaatagt tttgctaata aatgactgga
      721 ttttgtattt ataccataca cagaaatatc cttatttttc tttgcatcct tcaatgcttt
      781 ttttgcaaca acgtctgggg acactatgcc tgggaaattt tttgtccctt tttcagctac
      841 aactatacct ctcttaaata gatttgtact catccaacct ggacatacag caattgcatt
      901 tatcccttta ctcttaagct caacatttaa tgctcttgta taatttctta taaacgcttt
      961 agttgaacta taaatatttt gatatggcaa tggctggaat gatgcttgtg aagaaatatt
     1021 catgatatgg cttccctttt gcatatatgg aatatatata agacccaatg ctacaacagc
     1081 actaatattt aaatcaatca tattaataga ttcatctata cttaaatcat tgtatgaaca
     1141 gaattttgca aaaccagcat tgtttataag gtattttata catacatttt ctttacttaa
     1201 aaatgtacca atttctttta cttcttctat ttttgataaa tcttttgaaa aaacttttac
     1261 cttactgcca aattcatcta ctaattgatt taatctcgcc ttgtttcttg caactgccca
     1321 tatttctgtt atttcttctt catttattaa taactttaga aactctttac caaaaccact
     1381 attagcaccc gttataattg caatcttctt ataactcata attattctcc tttaccataa
     1441 gtttatacgc tcaaactgta aacttatttt ttacaaataa aacaccctct attcagatgt
     1501 tatattcata tcatttgaga tattatattt atatcatttt caatagttgt tatatagcat
     1561 aaaaaatagt gttaatatta tagaatcgat attgattata tttaaaaagt agtgttaatt
     1621 atattataga atcagtattg attatattgt aaaaagtagt attaataata ttgtagaatc
     1681 agtattgatt atattta
//
LOCUS       DAEMAE010000379         1669 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEMAE010000379 DAEMAE010000000
VERSION     DAEMAE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.386"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..598
                     /locus_tag="KPH93_003704"
     CDS             104..598
                     /locus_tag="KPH93_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690444.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYVGNKRNRSSSK
                     KNILKLKIPFSTFIQMPRKIENKDKINLKYLIQDMTSSILDDNNLFISVTAIISYENS
                     SKIE"
     gene            626..1573
                     /locus_tag="KPH93_003705"
     CDS             626..1573
                     /locus_tag="KPH93_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG1690445.1"
                     /translation="MRITKTTFKDVFSNDVNGSILKVEKYVDKKCASVFDILTYTVTV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLNPQQGFYIGRIDASCKKVIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISVESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCVEVEIDYSCKKR
                     SNFKTANKANDIQKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT          734 a          137 c          239 g          559 t
ORIGIN      
        1 ctaaaagtaa gtagtaactg aatcgtgaat aatcaaaaat ggtaatttga aatagttttt
       61 atgaatagta ttaatatata gatataggga gattgatgtg attatgtatg aaaataataa
      121 ggtaatatat aattttgaaa gatgtgaaaa gctagaaaat gctctttatt tatctcaatt
      181 taatacatat aaaaacttta agttaacttc taaaaaaact gtaataaact cattagatac
      241 atctacttat aatatgaata ttcatagcat aagtctcgtt gatactatgg aatgtatatc
      301 gtcagaagat gaacaaatga caggaaagaa gttgatttta gttggcgata tagatactga
      361 tgtaacatta gattatgtag gcaataaaag aaatagaagt tctagtaaaa aaaatatact
      421 taaattaaaa ataccattta gtacatttat acaaatgcct agaaagatag aaaataaaga
      481 taaaattaat ttaaagtatc ttatacaaga tatgacttca tctattttag atgataataa
      541 tttatttatt agtgttactg caattataag ttatgaaaat agctccaaaa tagagtaatc
      601 tttaaattat agaaaggtgg atgcaatgag aataacgaaa actactttta aagatgtatt
      661 ttcaaatgat gtaaatggaa gtattttaaa agtagaaaaa tatgttgata aaaaatgtgc
      721 atctgtattt gatattttga cctatactgt tactgttaca aatacgagta ggtataaaac
      781 tggaaatata tttttcaaag attatatctc taaatatata gagtttataa ataatactgt
      841 aaaagttaat ggtattataa agcgtgggct aaatccacaa caaggttttt acataggaag
      901 aatagatgca agttgtaaaa aagttatatc ttttaaaagt gtagttttac caaattctgc
      961 acataggatt ataaagaata gtgctaatat atactattac tataagtgca atctagataa
     1021 attcccaaca agaatatcag tagaaagtaa tagagtttat actaatgtaa ataaaattgt
     1081 attcaaacaa ttgaatataa gtaatacttt aaaagctcct aaaaatctaa gagatatatt
     1141 aaaagtaaat gtaaatagta aaatatttga tgttaaacct atgaaaaatc ttataaacaa
     1201 aggtagtaat ttgaaattat gtaacttgat agtttttggg tgtgtagagg ttgaaataga
     1261 ctatagttgt aaaaagagga gtaattttaa aacagcaaat aaggctaatg atattcaaaa
     1321 agataagtat tataaaaatg atatacaaaa taacaatgaa aatataaacg attgtaaaaa
     1381 gcaaataagt tttggtgata ataaggtaaa aaaaataaca aaaacttttg gattttcctg
     1441 tttcatttgt tctccagttg gaattattta tgaagatatg aaaaatataa atataaaaat
     1501 tgaacataca tcaataaatg aattaaatcc agaagaatta tttgtaaata catcactttt
     1561 attgtattat taagtatttt aaagctaaaa cttaaactta ttaaaatagc atttggctaa
     1621 aggaaaatag gtattgatat atttatcata tatataaatg attaattaa
//
LOCUS       DAEMAE010000380         1663 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEMAE010000380 DAEMAE010000000
VERSION     DAEMAE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.387"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            301..1593
                     /gene="brnQ"
                     /locus_tag="KPH93_003706"
     CDS             301..1593
                     /gene="brnQ"
                     /locus_tag="KPH93_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG1690446.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGAILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     DKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLISVLTVATSFGVK
                     IPLVNNLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          604 a          211 c          290 g          558 t
ORIGIN      
        1 aataaaacga ataatctaaa aaatcttaat gttttaatta ttcagaataa ttaattttat
       61 caagaaaaat tagagaaaaa acaaaaaacc aactatgttt aaagtataca ggttgatttt
      121 gtttaattaa gaaagttgta ttaaatattg tgaagatttt ataaattatg aaacataatt
      181 gacaaattta agtcaaaaat gtataattaa ataaaatctc gtcaaaaggt tttcatttaa
      241 tgaagagatt ggacttattt tttagtaaaa ggtagtaaaa ttcaaatggg ggtgctttaa
      301 atgggtgaga aacaaatcaa ttttaaagat gtaattgtaa ttggatttgc actatttgca
      361 atgttttttg gtgcagggaa cttaatcttc cctccatatc taggagtttt atctggaagt
      421 agctggctaa tagctttcat aggattcctt tttgcagatg gtggtttggc attattagca
      481 gttatagcag caactaagtt caatggagac acatcgaaga tgttctcaag agcaggcaaa
      541 ggattaagta ttgcattagg atgtgcaatg gtaatctgta tagggccact tttagcaata
      601 cctagaacag cagcaacaac ttatgaaatg ggtatattac ctacaatagg ttctggaata
      661 agcccagtaa tattctctat agtattcttt gctctagtat tggttttaac aataagacca
      721 tcaaaagttg tagatatagt tggggcaata ttaactccag ctttacttat agctttagca
      781 gtattaatag ttaaaggtat cgtatctcca ttaggagaaa taagagacag ttctttaata
      841 caaaatgtat ttgcagaagg tataactcaa ggttatcaaa caatggatgc attggcagct
      901 agtgtatttg catcaattat aattatgtca gtaatagcta aaggttatac aggtgataaa
      961 gaaaagatga aagccacaat aagtgcaggt gtaattgcag taataggaat ggcattagtt
     1021 tatggtggat tatgttatct tggagcaact gtgtctcaac aatatggaca agatgttcaa
     1081 caaacagctt taatagttgc aataacagca gctttacttg gaactacagg taaaatttta
     1141 ttggctatta tagttgcatt agcatgttta acaacagcta taggtctttc ttcagcagct
     1201 ggtcaatatt tctcaacact tacagatggg aaattaaagt atgagcatat agttatagta
     1261 gtttgtgtat ttagtgcaat catatcaaac tttggagtaa gtacaataat acaattctct
     1321 tctcctatac taagtatggt atatccagca actataactc tagttatact agcattattt
     1381 agtaataaaa ttaaaaatga caacgtgttc aaatgcgctg catacatggc attattaata
     1441 agtgtattaa cagttgcaac aagttttggt gtaaaaattc ctctagttaa taacttacca
     1501 tttgcttcat taggatttaa ctggatagtt ccagttgtaa tagcaggaat aataggaaac
     1561 tttattccat ctaaaagcag taaaactgca taataataaa aactaattga taattatgga
     1621 attgccttta aattatatag gcaattcctt tttatattta aaa
//
LOCUS       DAEMAE010000381         1608 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEMAE010000381 DAEMAE010000000
VERSION     DAEMAE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.388"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            173..1512
                     /locus_tag="KPH93_003707"
                     /pseudo
     CDS             173..1512
                     /locus_tag="KPH93_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
BASE COUNT          632 a          121 c          252 g          603 t
ORIGIN      
        1 aaaagtttaa ttcaatttag caaatttagt gatataatta aaaaagaaaa gaaatatgga
       61 cttatttgga ataattttga caatctattt actacacgtg tagagtcgta tataattaaa
      121 ttatacataa aatataaaaa aataatttag taatacgtta ggagaaagta ttatgataaa
      181 gtcaagctta ttttggaata ttttcattgc gatatccttt atgatagaat ggtttctttg
      241 taagaaaatg ctagattata caagtgagaa aaggatttct aacaaaaaat aaacttttgt
      301 atggtaggta ttattgtttt tgctgacata atatattttt ttgatgtacc tccaaatata
      361 agagtaatta tatctataat aattactatt gcattttata aaatctcata tgatgtgagg
      421 tttataaagt ctgtattaat atccttaatt tattggatgt tactaatcgc cacagatgct
      481 ttgagtatat ctttaacagt ttggcttaat tctataaatg acatgagtag tatagggacg
      541 ggagatatat acagaattga gtctataata ttgggaaaga tacttttgat tgtgtctttg
      601 tttttatata gagccttaaa gataaaactt aatatagaaa taaataaaag gacattattt
      661 tatatagtta ttcctatagt cgctaatata gcatcttttt ttgtcatttt tgaatacata
      721 tttaatccag ggtctggtag cttaatacat aatttccaat tcgttatgat ttctgttgta
      781 ttacttgtat caagtataac tttagtttta attgctaaga aattacagca atataatact
      841 ttagttgtag aaaacaatat gattaaaaat actttgaagt atgaaaatga gtattatgaa
      901 gatatagaaa agaaatattt aaaaactaga ttactaagtc atgatatgaa aaatcatata
      961 ttgtgtataa attctatgat taagaaaggt attgatgtta cttcttattt aaataattta
     1021 caggtagaaa ttcagagtaa tgacttgtta tttaatactg gtaattttat attagatgct
     1081 atattaagtg aaaagaaaga agtttgtgat aagaatgata tagaactaaa aataggaata
     1141 aattttagtt gttgtgaagc tgttgaatta gtagatatat gtcatatatt ttcaaatatt
     1201 ttagacaatg caattgaggc atgcaataaa attgatagtg gaaatagagt tatttgtgtt
     1261 aaaggtgatg ttattcaaaa tttttatcta ataagagttg aaaatactaa gattaataag
     1321 attattacta aaaatggaga tattttgaca gataaaaaag gttctcttca tggacttgga
     1381 ataaaaagtg taaaaaactc tgtaaataag tatggtggta atgttgtaat tgaatatgat
     1441 gataaaaaat ttgtagttaa aattgctatt ccacttagaa atgtgaagaa agcagttaat
     1501 catgtgattt aatgtatata aatttgatgt gtagaaattt aatatataag aattaatgta
     1561 taagaattta atatgtataa atgtgataca tagaagttta atatataa
//
LOCUS       DAEMAE010000382         1507 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEMAE010000382 DAEMAE010000000
VERSION     DAEMAE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.389"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            226..1491
                     /locus_tag="KPH93_003708"
     CDS             226..1491
                     /locus_tag="KPH93_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG1690447.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT          519 a          189 c          246 g          553 t
ORIGIN      
        1 tagtttaaag ggtatatttt tttgtttgtc gtttaatatt attatcaatg gaataatttt
       61 aaaatttatt taaataacaa aaattttctt taaattctgt caatttttca aaaactttgt
      121 taaataatat actttcatac atccattgaa aaaacaataa aaaagtaata ctatatatag
      181 tactagcatt aggcacaaaa taatacaaat ggaggtactt atattatgga atttcaacaa
      241 ataatagcta tatgtatatt tttaatagtt atggcagcta ttattacaga aaaggtaaac
      301 agatccgtag cagctgttgg tggtgcacta ttaatgataa tattcaatat acttacactt
      361 gatgaaggat taagccatat agatttcaac actattggtg tattagttgg tatgatgcta
      421 tttgtagctg ttgtcaaaaa ctcggggtta tttgaatata tagctatctg gactgcaaag
      481 aaagctaaag gtgacccatg gaagataatg atttgctttg ctattataac agctatttta
      541 tcagctgtat tagataatgt tacaactgtc ttacttattg gaccaatgac tatagttatt
      601 acacaaatat taggacttaa tccagttcca tttttaataa cacagatttt agcttcaaat
      661 ataggaggaa cagctactct tataggagac cctccaaata ttatgattgg tagtgctgcc
      721 aatcttagtt ttatggattt tgtaataaat ttaggtcctg ctgttatagt aatacttgct
      781 ataactataa tatgctttag atttatttat ggaaaagaac ttgttgtgaa tgaaagagct
      841 aaaaatgcaa tattaaaatt agatgaaaaa aaatctgtta aagataaacc tctacttata
      901 aaaagtctta tattaatagc attcatactt tttggtttta tgtttcacag tacaatccac
      961 atagattctt ctgttgtagc actgacagga gcatctataa tgcttttaat aggaaaacag
     1021 gatgtagatg aaattatggc aggtattgaa tggtctacaa ttttattctt tatgggatta
     1081 tttgtagtag ttggtggatt agtagaagtt gggataataa acaaattggc tcaggcattg
     1141 attgggctta cagaaggaca tcttgtattt acaatgttat tgatattatg gctatcagct
     1201 atagtctctt ctttcttaga taatattcca tttgttgcaa cactaatccc acttattttg
     1261 actatgcaag ctgaaggtat tgatgtaatg ccactatggt gggcaacatc tcttggagct
     1321 tgtcttggag gaaatggaac actaatagga gcatctgcta atgttgtact agcaggtata
     1381 ggtaataaac acggtcatcc tatctctttt aaagagtatt tcaaaatagg ttttccactt
     1441 atgattatat ctattattat atctactgtt tatttaatta taaaatttta aaaaatatag
     1501 tataata
//
LOCUS       DAEMAE010000383         1505 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEMAE010000383 DAEMAE010000000
VERSION     DAEMAE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.390"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..903
                     /locus_tag="KPH93_003709"
     CDS             58..903
                     /locus_tag="KPH93_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690448.1"
                     /translation="MNIIKNITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KNHLN"
     gene            complement(1127..1291)
                     /locus_tag="KPH93_003710"
     CDS             complement(1127..1291)
                     /locus_tag="KPH93_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690449.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          631 a          184 c          152 g          538 t
ORIGIN      
        1 ttcaatattt gatgctagaa tataattaat aaaaatttaa ttataggtga ttgtattatg
       61 aatattatta aaaacataac tttactctta acattattaa ttgtgatatt aatgacagga
      121 tgttccccaa gagaaagtgc cagtgaagtt gcaaaaaaat atattgataa gatgaactct
      181 ggtgactatg aaacagcata tagtttatta agtaaagata gtaaaaaaaa aattagtata
      241 gaaaatcttg aagaatttca aaatatttta gttagtacaa aaagaataat tagttataaa
      301 ataggtaaag aaaaaatcat aaaaaaatat aaacatgatg gaaaagaata taaaaatgtt
      361 gttaaattag aagaaacttt taatataaaa aaccttcttt ataaaaaaga tgattctcta
      421 aaagtaaata gatattttgt agttgaaaat aacaaataca agctattatt gtatgaaagt
      481 ttcaaaaaag aatttggtat taatagtatg gaccttgctc aacttaagct taatgatttt
      541 ggactaaaat actttaaaga agttgacctt atactaaaaa aagctattgc tattaatcct
      601 actgattcaa agctttatta tgcccaagca tgtaattata tgaattttga tggatttcct
      661 ggttattcag atacaacata taatgcttta gattcaatta ataattcttt aaagtattta
      721 gataaaaatg attctgacta caaaaaaaat gcaagtaata tatataactt aaaaggagtt
      781 attcttatgt ttcacaaaag atttgatgaa gcacaacaat acttaaataa agccttatac
      841 tttaacaaag ataatgaaga cccaaagtat aatctaaata caataaaaaa ccatctaaac
      901 tagatattta atttttgaat ctataaaata tttttttata tataattcag tactgaacca
      961 caatctctac taaactaatt actagtttaa attataaaga cttatttcta tataaatatt
     1021 actctataac aataagtttc ttatttaaat tttaaaatat tatctaaaca tcataaaaaa
     1081 gaaggtataa agagctaccc tcaagctcaa tataccttat aaatatctaa acttcatttt
     1141 ttactaattt ttctatatct ttgataatat gttctttaga tttttttcca gctatggcat
     1201 caaaaattgc aacagccttt ttctctccaa acttttctgc tacatcatta aagtctaaaa
     1261 atctaacact ttcttttggt ttaccaaaca tataacacct ctcatacatt tttatatatt
     1321 tattataact tctatagact tatttttcac caataaacgt tagacaaatt agtcccttta
     1381 tttttttaaa ctagctttac cttcaaaatt cttaacaaaa acagtttatt ctactctata
     1441 aataaaaata gacaatttaa tttgtaggaa actgataata gaatacagta aataaaactt
     1501 tgttt
//
LOCUS       DAEMAE010000384         1502 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEMAE010000384 DAEMAE010000000
VERSION     DAEMAE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.391"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..438
                     /locus_tag="KPH93_003711"
     CDS             154..438
                     /locus_tag="KPH93_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690450.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(793..1008)
                     /locus_tag="KPH93_003712"
     CDS             complement(793..1008)
                     /locus_tag="KPH93_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690451.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1019..1486)
                     /locus_tag="KPH93_003713"
     CDS             complement(1019..1486)
                     /locus_tag="KPH93_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG1690452.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          611 a          173 c          177 g          541 t
ORIGIN      
        1 aatatacatt taaaaattac aatttaaaac attaaagaat aaatttgtat tttgttttat
       61 ataaagtaat ttgaagaata tggaaaaaca tgattgctac attattaatt agggtataaa
      121 atggtataaa tagaatgata aggggtggat gaaatgcttg tatataataa aagtttttat
      181 cctaatgaca tatttccaag attagatttt tcaaaaataa aaaaacagtt aaaactgata
      241 gataatgacc tgtcagattt tggaagcata tgtataatag aaaaagaaca ttatacaata
      301 agtgtaaaca gtataggtga aataaatgtg tattatgatt tagagtacga aaataaggtg
      361 tatagaatag tttatgagat tgaaaagtta tttaaatctc aagttggaag gtttagtata
      421 tctacataca gaaactgata attaaaaaag tagaaattaa aaaacttaat actaaagata
      481 tagataaata tatgaggtaa cagctagaga gagttgttac tctttttaaa tactaagtat
      541 atattattct tattaatttt ttgtaaattc actaaaagac ctttttatag aatattattt
      601 tcttttttta ttaaataggt aaatcagtaa aaatgcaatt ccagttccaa ttattatccc
      661 aatgatattt tctataatta atttttttaa tataaaagta aataataaaa atgctataca
      721 taaacctata atatagtaaa agaataaaaa tcatatagta taataaaaaa gagtgtctct
      781 aaacactctt ttctaatcat tttcatcttc tacatattca agtatatcac ctggctgaca
      841 gtctaaagct ttgcaaattt cgtttagagt ggtaaatcta atagcttttg cttttccagt
      901 ttttaaaatt gataaatttg catttgtaat atctattttt tctgccaaat ctttaagtga
      961 tattttcctt tttgccatca ttacatctag gttaactact attgccataa catcacctct
     1021 atatcgttaa atcattatct tcttttattt ttatagcttt atcaaatgtt tctgcaatta
     1081 caaagcacat tagtgcgaat ataaaagaaa taaccataag tggtgagata ccgccactat
     1141 tatctccaaa tatcattata agactagtat ttgagttata accaattaca cattcaatta
     1201 tagtgtttat caataaacaa catcccatta ctttaaacct ttttacattt tcaagtatga
     1261 atggggaata cttagtacta ttaattataa atatcaagaa taagatgact ataaagtagt
     1321 cagaaaaata tagtattctt gcaattaaag cgattacttt gtcagaacta aaatcatata
     1381 aaaacatagg tatcatacat aagaatatta taccagcaac tcctaaaagt gataatagaa
     1441 aatatagaaa tactgataat atattactaa aaaattttgt aatcataaaa tatcatctcc
     1501 tt
//
LOCUS       DAEMAE010000385         1468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEMAE010000385 DAEMAE010000000
VERSION     DAEMAE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.392"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            329..1345
                     /gene="gap"
                     /locus_tag="KPH93_003714"
     CDS             329..1345
                     /gene="gap"
                     /locus_tag="KPH93_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG1690453.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
BASE COUNT          627 a          159 c          253 g          429 t
ORIGIN      
        1 aataaaaatt tatttttatt attttaaatt agaggagatt cttgaatata tattattttt
       61 aaaaataagt atagtttaaa aattcaaaaa aaagatggca aaatatgtcc caagggacat
      121 tgagcaaaat aaatgatttc agcttcttca cactaaaaag tacatactaa atgaggggta
      181 aaataaagaa aaatgatata ctaattgtct tttgataata aaaaaacatg gattttgatt
      241 atttaatcaa gaaaaagtat tgaacaaatg atttatttag tgtacaataa tcatgtaaaa
      301 acatagtaat ggagggagac atttcataat gaaaattaag gtaggaatga acggatttgg
      361 aagaataggc agagctgtat taagaatagc tcaagaagaa ttaggagata atatagaaat
      421 tgtagccata aatgcaagag caacaacaga atctttggca catttattta catatgattc
      481 atgctatgga acttttagag gtgaagtaga agcaaaggat gaagatactc tagtagtaaa
      541 ctctaaagag ataaaaatat taagatataa tgacccagaa gaattacctt ggaaagaatt
      601 gggggttgat atagtaatag aatcaacagg attatttaca caaagagaaa aggcagaaaa
      661 acatattaaa gcaggagcaa aaaaagtaat tataacagct ccgggaaaaa atgaagatat
      721 aactatagtt atcggagtta atgagaaaga atatgataat gaaaagcata atataatttc
      781 aaatgcatct tgtactacaa attgtctagc tccttttgct aaagtattgg acgaaaaatt
      841 tggtatagta aaagggttaa tgactacagt acattcatat actaacgatc aaagaatatt
      901 agataaaagc cataaagatt tgagaagagc aagagcagcg gctgaatcta taatacctac
      961 cactacagga gcagcaaaag ctgtgtcaag agtattacca caattagaag gaaaattaaa
     1021 tggtttttca cttagagtgc caacaccaac agtttcactt gttgatttag tatgcgaact
     1081 taaagaaaac gttacatctg agcaagttaa ctctgtacta aaagatgctg ctgaaggaga
     1141 actaaaaggt gttttaggat attgtgataa gccactcgtt tctatagact ataaaggaga
     1201 ttcaagatca tctattatag acgctctatc cacaatggtt atagaagaca atatggttaa
     1261 ggtagtttct tggtatgata atgaatgggg atattcttca agaactgttg acttagtaaa
     1321 atatgtagct gaaaaattaa aataatatat atttcttaaa acaaacttta aaaaaatttt
     1381 ttaaaaaaat tatataaaat taaaaaaaaa gattatgaaa aatattgaag gaaaaatttt
     1441 ttcataatct tttttctttc atgcaaaa
//
LOCUS       DAEMAE010000386         1465 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEMAE010000386 DAEMAE010000000
VERSION     DAEMAE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.393"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1231
                     /locus_tag="KPH93_003715"
     CDS             92..1231
                     /locus_tag="KPH93_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1690454.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSVNEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1257..1445
                     /locus_tag="KPH93_003716"
     CDS             1257..1445
                     /locus_tag="KPH93_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690455.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          639 a          124 c          217 g          485 t
ORIGIN      
        1 aaattgagtt tgataattat aaaacaaata atcaaaaata gtgtaaaaat taatactttt
       61 tgttaatatt aatagataaa gataatctaa ggtggtgatt aaactgacaa tagaagaata
      121 caaaaacaaa tactttactg gaaataaaat cgtactttct ctaaatagtt ttgataataa
      181 agaactatac tatcatgaaa ctgaaaatat attaaattta ataaatgaaa tagataaatt
      241 aacatcaagt aagatattta aaacaataga agataaaaat gatttggcac tagagtctta
      301 ttattctagt gtaatagaag gtgctttttc aactagaaag attgcaaaat ctataattag
      361 aggaaagatg aaaccaagta ataaaagtga atatatgatt tacaataatc atagagcctt
      421 agaatatgga ttagataact tagatgaatt gtatagtcac aaatttatat atgatttgca
      481 tcatatattg ggcgaaaact gcttggattc agaagaatat gagtatagaa ctgaaaaagt
      541 atatgtatgt gattcaaaag gtgagattat acatacagga cttgaacctt tgaagatata
      601 tgactttatg agtaaattaa tagattttat ggaaaatagc aaagtaagta atttaattaa
      661 aagtgctata attcattttt actttgtata tgttcatcct tttagtgatg gtaatggaag
      721 aacatcaaga gccttatcat atctatattt gattgataaa ggatatgata ctttcaagga
      781 gttttcaata tcatatatga tatctaaaaa cagaactaag tattataagg ctatacttga
      841 tgtagaaaac aaaggaaata atttaactgt atttatagag tttatgctca agtctataat
      901 acaatctgtt aatgagatgc gtaatatgca tgatagaaaa tctttagaaa gtatattaaa
      961 agaagaattg tttgaaaatg acataactct atcagcaaca gaagaacata tattaaaata
     1021 tatttgcaat aaagataact attctatgac attagaaaat tatataaaaa agaataaaag
     1081 tagatactta aaggctggaa taaaggaaat tgaattagtt gaccagttga tggaagtgtt
     1141 taataatctt gaagagatag agattttgtc taaagaaaaa aatatatata aagttaatga
     1201 taagtactta aaaatgcttg atatagacta atttagataa tctggagagg gaatatatga
     1261 ataataagaa aaaggcattt ttgatttctg gggctataaa cgtaatttca ttaattattg
     1321 ttttttcatt gtttcattat aatattatag aaaatagcac acaaaatatg ataataactg
     1381 taatcttaat ttttattttt gaaatgataa aaataaaaat catagagaaa tactataaac
     1441 tataaacaag aaatatcttt tctaa
//
LOCUS       DAEMAE010000387         1462 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEMAE010000387 DAEMAE010000000
VERSION     DAEMAE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.394"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..1223
                     /locus_tag="KPH93_003717"
     CDS             111..1223
                     /locus_tag="KPH93_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG1690456.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT          536 a          177 c          262 g          487 t
ORIGIN      
        1 ctagttttat aatataatta taagctatta atattataaa gccgatatta ctaatataat
       61 aattataata cttatagtta atttactttt gttgaaaggt ggtaaataac atgaaaattt
      121 cagaagtcgc tactccaaat tttttgctag atttagacca gttagaaaag aatatagata
      181 aaattcaaca aatttgtaca aataataata aacaactttg gcctatgctt aagacacata
      241 aaagtactta catagcgaga cttcaaaaaa atgcaggtgc tagtggtttt ctagttggaa
      301 cacttgatga aggtgaagct ttgataaaag caggttttga agatattatg ttggcttatc
      361 cttacatggg agaggtcaat ataaatagaa ttgtgactat ggcatcaaaa ataaatttaa
      421 tttgtgctac agataatata gaatgtgcta aggcttatag tgaagctttt tctaaatcaa
      481 aaatagagtg caagctactt attattgtag attgtggttt acaccgtttt ggggttgaac
      541 cagaaaaagt ggttgaactt gctactgaaa tttctaagct agaaaacatc aaaattgttg
      601 gtataagttc acatccaggt caagtgtatg gttgtagttc tccagaaggt gttccagatg
      661 tttgtgttca agaagattct tctatggaga aagcatacaa agctctaact gataatggat
      721 ttaatataga aattgtcgct tctggtagta ctccaacagt tgaagctgaa gctgcttcta
      781 aaactataac tgctgtaaga cccggaaact atgtatacta tgatgctata cagataggtt
      841 taggttgtgc tactgaagat atgtgtgctc ttactgttgt gtgcactgta atctcaaaaa
      901 atagcagtgg atactattta attgattgtg gaagtaagtg ccttggactt gataaaggtg
      961 cacatggaaa tagtagtatt gttggttatg gtagggtatg tggacatcca gaacttacta
     1021 ttgattcact ttcagaggaa gttggaaaaa ttaaagtaaa tggtgaaact gatataaaaa
     1081 ttggtgatag aataagaatt atacctaatc attcttgttc tactgcaaac ttgacaagta
     1141 atttattagg ttttagagga gatacaatag aaaaagtcat agaagtagat attcgtgaaa
     1201 attcaaaaca aatcatcctt taaaaacaca tttattttga tattaacatg aaagtatatt
     1261 tttcttaaaa ttattgttga taactaatgt taatgttgta taaataaaaa acataaattc
     1321 tagaaatata aatttaaaag aagaggctgt ctcaaaatat agataagcct ctctttcatt
     1381 gttgaaatta tgaaaatata cttgatttca tacaaaaaga gcatctcatg aactaaaaag
     1441 ttcattttga gatactctct tc
//
LOCUS       DAEMAE010000388         1456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEMAE010000388 DAEMAE010000000
VERSION     DAEMAE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.395"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            314..571
                     /locus_tag="KPH93_003718"
     CDS             314..571
                     /locus_tag="KPH93_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690457.1"
                     /translation="MIIIRSQDKTNLMHINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(680..1360)
                     /locus_tag="KPH93_003719"
     CDS             complement(680..1360)
                     /locus_tag="KPH93_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG1690458.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVVIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSSLFRVDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVLLTGLGFSLAIEFCQLFNLRATDIDDLMMNTLGAILGYFIWVGVN
                     NLFNLIKRKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          617 a          164 c          156 g          519 t
ORIGIN      
        1 ataaacaata tgtataaaaa tttttgaaag taaaattacc aaatgtataa aataaatcag
       61 tagcatagat aattttatgc tttaatagat tatctaattt agttataatg gtcaaaaatg
      121 caatctacaa ataaaacaaa gtattatcaa aaaaagtatt ctttaagaga attttttatt
      181 agatatgtaa ttgatataaa taatcgtatt aaaaaataaa atactaagtt taaaaattta
      241 aataatctac aaatcaatta tgaaatatta tttcttagaa attaaaatga attaagtaaa
      301 tggaggaatt gatatgataa taattagaag tcaagataaa actaatttaa tgcatataaa
      361 ccaaattaaa atagatggta gtcaagttta tgcagtattt gaaagtaaaa ttgatactgt
      421 aaaaatagga gattatgaaa ataatacaag agctatacaa gtgttggaca atatacaaaa
      481 cttcattgaa aatggaacaa aatatgatta tataacatca aataaggtca gatacaatgt
      541 taataaaata tttcagatgc cagctaaata gtgagtgtgt attttagttt aataactaaa
      601 gcccactttc ttttgtagta tgaaagaggg ctttacatat ttgtaaatat cgaagtacat
      661 ttgtttatca ataaattatt taataaggta taatggacca attaaacaat aaaaattctc
      721 ctaaaacagc aagtattaaa tatataatag cttcattttt gtaaagagag gtaatttttt
      781 tgcttatatc atcatcaatt gtaatagcaa tttcatcttg gataatattt ttatatatgg
      841 atggaaaaat atcttttctc tttataagat taaataaatt atttacacct acccatataa
      901 aatatcctaa aatagcacct aatgtgttca tcattaaatc atcaatatct gtagctctta
      961 agttaaacaa ttgacaaaat tcaattgcta atgaaaaacc taatccagtt aataaaacat
     1021 tgataatatt tctaaaattt ttccaaatta aaggtagtaa aaatcctaaa ggcataaaca
     1081 taattatgtt taaaatatat gttaaaatgc caactgagtc aaaaggtatt agatttattt
     1141 catcaactct aaaaaggcta ctataccttc ctatatccca tatgctacca atgccagctt
     1201 tactaagtgc taggtatata tataataaaa aaatatatac ccatactaaa tgtatcacta
     1261 cattcttttc aactttcaaa ttttttcttt ttataacaac tatttggtat attaagcatg
     1321 gtaatataac gcaaataaat gaataaaatg tccatatcat tttattttgt cccctttcaa
     1381 atttatgttt ttagttttat aagtactatc tttatattaa actgtaagtt ttaaaaatct
     1441 tttaataaaa tcttaa
//
LOCUS       DAEMAE010000389         1453 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEMAE010000389 DAEMAE010000000
VERSION     DAEMAE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.396"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..>1453
                     /gene="cls"
                     /locus_tag="KPH93_003720"
     CDS             14..>1453
                     /gene="cls"
                     /locus_tag="KPH93_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG1690459.1"
                     /translation="MSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIP
                     ALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSDIHQHKDMI
                     YALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKDDKIGTEII
                     DILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYR
                     NHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWR
                     YTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLKMIQKARKY
                     IYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAK
                     IYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFE
                     NDILNSKEITLDIYNSRSTYIKIKESISRL"
BASE COUNT          555 a          181 c          229 g          488 t
ORIGIN      
        1 atcatttttc gaaatgtcaa tatatgaaat agcagttact acaatatata taattaattt
       61 catggttata ctaaacctta tctttagaga aaaaagaaac ataaatacaa ctttaacatg
      121 gcttcttata ttagtattga ttcctgctct tggattcata ctttatatag cttttggtag
      181 aaatatttca aaaaacaata tgtttagatt gaaagaaaag gatgataaga taatcaaaag
      241 taatatacta gatactcagg ttaaactcca atctacatct gaaattgatt cagatataca
      301 tcaacataag gatatgattt atgcattggc taattcaaat aatgctcatt ataccaataa
      361 taatgatgtg tggatttatg ctgaatctag tcaatttttt aacagtcttc ttgaagaact
      421 taaaaaagct aaaaaatata taaacataca attttatatt ttcaaagatg acaaaatcgg
      481 aactgaaata atagacattc ttatagacaa agctaaagaa ggtgttgagg taagactttt
      541 atttgatgca gttggtggta gaacactaaa aaatagtaca ctttccagac ttaaagaaag
      601 tggtgttaaa gttggaagct tcttcccatc atttttgaaa attgtaaact ttaacttaaa
      661 ctatagaaat caccgaaaaa ttgttgtcat tgatggtaag gtaggatttg taggtggata
      721 taatgttggt gatgaatatt taggtagaaa tccaaaattt ggtctatgga gagatacaca
      781 tactaaactt actggtgatt gcgtaattga cctaaatatg agattcatcc ttgattggag
      841 atatacaact aaagaagatt tagatttaga aaagtacttt atagaagaag atacaaattc
      901 taatcatcct tctgaaaata ttgggattca gatagtatca agtggtccag atatatcaga
      961 acttgatgag attaaatatg gttaccttaa aatgattcag aaagctagaa agtatatata
     1021 catacagagt ccttatttaa tacttgactc aacttttata gatactttaa aaatagcttg
     1081 tttatctggt gttgatgtga gagttatgat tccttctaaa cctgaccatc catttgtata
     1141 ctgggcatca tattcatatg ctggagaact tttaaagttt ggagctaaaa tctacactta
     1201 tgggcaaaac gcatttttgc atgctaagac aatagttata gatgactcta tatgctctat
     1261 aggaacagct aatatggata tcagaagttt tgagcttaat ttcgaagtta atgcatttat
     1321 gtactcttca aaaaaagcat tggaacaaag aattatattt gaaaatgata ttttaaattc
     1381 taaggaaata acattagata tatataattc taggtctacc tatataaaaa tcaaggaatc
     1441 aatttcaaga cta
//
LOCUS       DAEMAE010000390         1447 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEMAE010000390 DAEMAE010000000
VERSION     DAEMAE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.397"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..230
                     /gene="rrf"
                     /locus_tag="KPH93_003721"
     rRNA            114..230
                     /gene="rrf"
                     /locus_tag="KPH93_003721"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            238..312
                     /locus_tag="KPH93_003722"
     tRNA            238..312
                     /locus_tag="KPH93_003722"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:270..272,aa:Asn,seq:gtt)
     gene            318..403
                     /locus_tag="KPH93_003723"
     tRNA            318..403
                     /locus_tag="KPH93_003723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:352..354,aa:Leu,seq:taa)
     gene            419..494
                     /locus_tag="KPH93_003724"
     tRNA            419..494
                     /locus_tag="KPH93_003724"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Met,seq:cat)
     gene            502..576
                     /locus_tag="KPH93_003725"
     tRNA            502..576
                     /locus_tag="KPH93_003725"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Glu,seq:ttc)
     gene            585..658
                     /locus_tag="KPH93_003726"
     tRNA            585..658
                     /locus_tag="KPH93_003726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Gly,seq:tcc)
     gene            663..738
                     /locus_tag="KPH93_003727"
     tRNA            663..738
                     /locus_tag="KPH93_003727"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Val,seq:tac)
     gene            744..820
                     /locus_tag="KPH93_003728"
     tRNA            744..820
                     /locus_tag="KPH93_003728"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Asp,seq:gtc)
     gene            831..905
                     /locus_tag="KPH93_003729"
     tRNA            831..905
                     /locus_tag="KPH93_003729"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:863..865,aa:Gly,seq:gcc)
     gene            915..988
                     /locus_tag="KPH93_003730"
     tRNA            915..988
                     /locus_tag="KPH93_003730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:949..951,aa:Arg,seq:tct)
BASE COUNT          437 a          265 c          323 g          422 t
ORIGIN      
        1 tcattcatta agatatatgt gtagttttta gagtgttaac tctaaagaaa ctagtgttac
       61 tagattctaa aaaattatta aaaacttaat agaaatatta agcataaaga ttatgtggtt
      121 acaatagcag agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg
      181 ctgatggtac ttggtgggaa actgcctggg agagtaggac gtagccacgt taaatgttcc
      241 aaggtagctc aacggtggag caaccggctg ttaaccggta ggctgtgggt tcgagcccca
      301 cccttggagc caaaaaagcc gaagtggcgg aactggcaga cgcacaggac ttaaaatcct
      361 gcgggactta cctctcgtac cggttcgatt ccggtcttcg gcacttatga aacaatatcg
      421 cggggtggag cagttggcag ctcgtcgggc tcataacccg aaggtcgtag gttcaagtcc
      481 tgcctccgca accattaaaa tggctcgttg gtcaagaggt taagacaccg ccctttcacg
      541 gcggtaacag gggttcaatt cccctacgag tcaccattaa atatgcgggt gtagctcaat
      601 ggtagagttc tggccttcca agccagctgt gagggttcga tccccttcac ccgctccaaa
      661 atgggaccat agctcagctg ggagagcacc tgccttacaa gcagggggtc acaggttcga
      721 gccctgttgg tcccaccatt tgcggcctgg tagttcagtt ggttagaatg ccagcctgtc
      781 acgctggagg tcgagggttc gagccccttc caggtcgcca aattaaataa gcgggaatag
      841 ttcagtggta gagcgcaacc ttgccaaggt tgaagtcgcg agttcgaatc tcgtttcccg
      901 ctccataata atttgggtgc atagctcagc tggatagagc aacgcccttc taaggcgtgt
      961 gtccggggtt cgaatccctg tgcgctcatt aagaaaagtt tactataatt tatagtaaac
     1021 ttttatattg tttaaaaaga cacaggatac atgtatatta cctgtgtctt tttgtttata
     1081 aactttaaac tatgtaaaaa ttacagtata ttcattaata ttgtaaacat acgaagggta
     1141 atgtataata gtctacagga tgtttatgaa ggtaccagtt aaatataact aactggataa
     1201 gaattatata cattatgttg tatactttat ggtatttata tattgaaata atttatgaaa
     1261 ttatttataa agagattcat gagcatattt tttgtctaaa cgataaggat aagaaattat
     1321 aaaaaaatta gagaatataa tgaattgata atttaatgta atatttataa gttatactaa
     1381 ataaattatt atactgtgaa atagatatat aactttaggt attgtgtatt attttcattg
     1441 tagaaga
//
LOCUS       DAEMAE010000391         1445 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEMAE010000391 DAEMAE010000000
VERSION     DAEMAE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.398"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            42..440
                     /locus_tag="KPH93_003731"
     CDS             42..440
                     /locus_tag="KPH93_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1690460.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSPKEMSERYKLNARNVK
                     INLNIKCHKFLPYVKNLQASSTNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFS
                     NMKSIYLKQTNGNLIELSNHRITESKPWKI"
     gene            428..1366
                     /locus_tag="KPH93_003732"
     CDS             428..1366
                     /locus_tag="KPH93_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG1690461.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVSIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLQKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSAFSIITVFLIIFILKQNNLI"
BASE COUNT          522 a          179 c          199 g          545 t
ORIGIN      
        1 tataaattta actaatttat ataaaatctt aaggagttgt catgataaaa aaaatagagc
       61 atgtggtcat tactaataat aatatagaaa attatatcaa tttttataaa aaatttggct
      121 tttcacctaa agaaatgagt gaaaggtata aacttaatgc tagaaatgtt aagataaatc
      181 ttaacataaa atgtcataaa tttttacctt atgttaaaaa tttacaagca agtagtacta
      241 acttatgctt taaaataaac acaaatattg atgagccaaa aaaatactta gaacaaaatg
      301 agattcaaat tgagcgagga attgtagaat gtacaggagt ctttagtaat atgaaatcta
      361 tttatttgaa acaaactaat ggaaatttaa ttgaattatc aaatcataga atcacggaga
      421 gtaaaccttg gaaaatttaa tcctatcaat aaacgttgtt cttcctttgt ttctcacaat
      481 gtcacttgga tatgttctaa aaaaactagg tctatttgat actcatactt taaataaaat
      541 gaataatgtc tgtttcaagt ctttcttgcc attattatta ttttttaata tatacaatac
      601 agatttagat ggttctctta attttaagct tatggctgta gctgttataa ttattttttc
      661 aatcttctta atcttagtct ttattattcc taaaattgaa aaagataata agacaagggg
      721 tgcattaata caaggtgtat ttcgtagcaa tttcgttata tttggtattc ctgtgacaat
      781 ttcactattt ggcgataaga gtattggagt tacatcttta ttaattgcag ttattgtacc
      841 tatgtttaat gtattgtctg ttgtaatact tgaaattttt agaggtggca aagtaagtat
      901 aaaacatatg attaaaagtg tcttaacaaa tccactcata atcgcctctt ttataggatt
      961 gacaatgcta attttaaata ttaatatacc tacattttta caaaaaacta ttggtgatat
     1021 ttctaaaata gctactcctt tatcacttat aattctaggc ggctctttta gtttttctgc
     1081 tataaagggg catataaagc ccattatatt gggggtatgt ggaaaactta tacttgtacc
     1141 tgctatattt ttaccaatca gcatttttct tggatttaga aatgtagaat tagcttcact
     1201 tatgataatg ttatctgctc caactgcagt ttcttcattc acaatggcac aacagatgga
     1261 ttctgacagt gaattagctg gacaaattgt ggtatttact tctgcatttt ctataattac
     1321 agtattttta ataatattta ttttaaagca gaataatctt atttaatgat atttgataac
     1381 acaaattggt attatgaaaa acatatataa aattgattta gttgcgtaaa aacacattat
     1441 tatac
//
LOCUS       DAEMAE010000392         1408 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEMAE010000392 DAEMAE010000000
VERSION     DAEMAE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.399"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            407..1114
                     /locus_tag="KPH93_003733"
     CDS             407..1114
                     /locus_tag="KPH93_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1690462.1"
                     /translation="MIFTKSNKYDKDFLMENMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISVDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDGIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          589 a          127 c          246 g          446 t
ORIGIN      
        1 gtaaagtgtc aaatatgcac taaattaaat aagttaatta gtatatagct taaagtggaa
       61 gtaattaaaa cttattaagt atcagaaaac gacttcttat tagagtaagt atctaaaatg
      121 atataaaaat tatgattact cattaaagtg actattaagt agataaaatt acccattaaa
      181 ataactatca tagagtggat aaaattactc attaaagtga ttattgtaga gtagataaaa
      241 ttactcatta aaataactat catagagtgg atgagattac tcattaaaat tgcctgttaa
      301 agaatgaata aaaaagagat tgctcattat aagaagcata taaaaaataa aaaatcaaac
      361 gtataaaatt taataaataa taacggataa ggaaaggaaa gatagaatga tatttacaaa
      421 aagtaataaa tacgataagg actttctcat ggaaaatatg atgggaccta attgtataaa
      481 aatattggaa gaattaacaa gtaagataaa actagaaaag ggaatgcgta ttttagactt
      541 aggttgtggt aagggtatat cctcgatatt tttagctaag gaatttgatg ctactgtatt
      601 tgcaacagat ttatggatag aaccaactga aaactataaa agatttaaag agtttaaatt
      661 ggatgataaa atatttccaa tacaggcaga ggcacatgaa ctaccttatg cagaaggttt
      721 ctttgatgcg gtgataagtg tcgattctta tcactatttt ggaaacaaag aaggattttt
      781 agacaatcat atatcacctc ttgtaaaaga ggggggaata cttgctatgg caatgcctgg
      841 actaaaagaa gattttgtgg atggtatacc agatgaactt attccatttt ggcaagataa
      901 tatgaacttc cattcaatta catggtggaa taaactttgg tctgaatctg agtctgtgat
      961 tgtagagaaa tgtgaagctt taaattgtca tgatgaagct tggaaggatt ggataaattg
     1021 cgataattca tacgctataa atgacaaaaa aatgatggaa gtagagaatg gaaagtattt
     1081 taatacaatt tctttgatag caagaactaa ataattgtga tgaatttata aaagtataag
     1141 aagcatagaa atataagttg aattaaagta gaagttaaat aaaaatgtaa gttaaatgaa
     1201 gagtttatac aaaaatgatt ttttagatta tttataccca atcgagttgt ttaagtcagt
     1261 tgggtatttt tattttttaa agttaaattg caaatactat tgtgtgatat atattttaat
     1321 actaaatgtt tgttctatag gaaataaact aatagaaaaa ataattttaa caatgaaagc
     1381 aaaaaaggta ttgatgaaga ttaaaaat
//
LOCUS       DAEMAE010000393         1383 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEMAE010000393 DAEMAE010000000
VERSION     DAEMAE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.400"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..464
                     /locus_tag="KPH93_003734"
     CDS             <1..464
                     /locus_tag="KPH93_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1690463.1"
                     /translation="INDFEYFRNTGNKTLDIILGEKIYLCKKYNIEFEDSINISKLQF
                     IQDVDICAIFANALDNAIEACMKMNNNTEKRIEVKATYVNGFAIIKFINTKVNDIKFI
                     DERIKTSKDNNRVHGIGLASIKYMVSKYDGELIVNYSDNEFILKIMIPIKA"
     gene            477..644
                     /locus_tag="KPH93_003735"
     CDS             477..644
                     /locus_tag="KPH93_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG1690464.1"
                     /translation="MKKIALSLLKSMSTLSLGISRLSANTASSWIAHQAEEPQALKKL
                     KKNNTSTKFSL"
     gene            684..1268
                     /locus_tag="KPH93_003736"
     CDS             684..1268
                     /locus_tag="KPH93_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG1690465.1"
                     /translation="MFKSLSYKFANILVRNEVIEDEDFEIYRYGFETLVYFIINISVA
                     LLIGIALNKFIQTIIFLVCYCTLRQFTGGYHARNYTECTITFALIYISIILVTKNIDI
                     YKFKYILILLLLLSTVIIHKVAPLEHRNKPLSIYEKKNYRNIIKKITLSIIIVVTISL
                     IFNIMSEYIIYSSLAVFLITILLLVQIIINFFKK"
BASE COUNT          585 a          126 c          197 g          475 t
ORIGIN      
        1 aaataaatga ttttgagtat tttagaaata caggaaataa aacattagat ataattttag
       61 gagaaaagat atatctttgt aaaaaatata atattgagtt tgaagacagt ataaatatat
      121 caaaattaca atttattcaa gatgttgata tatgtgcaat atttgcaaat gcactagata
      181 atgcaattga agcatgtatg aaaatgaata ataatacaga aaaaagaata gaggttaaag
      241 ctacatacgt aaatggattt gccataataa agttcataaa tactaaagta aatgatatta
      301 agtttattga tgaacgtatt aagaccagta aggataataa tagggtacat ggaataggtc
      361 ttgcaagtat caaatatatg gtaagcaaat atgatggaga acttatagtt aattactcag
      421 ataatgagtt tatattaaaa ataatgatac caataaaagc ttaggaggaa gtacaaatga
      481 aaaaaatagc attaagttta cttaaaagta tgtcaacatt aagcctagga atatctagat
      541 tatcagcaaa tactgcaagc tcatggatag ctcatcaagc agaagagcca caggctttaa
      601 aaaaattgaa aaaaaataat acaagtacaa aattctctct ctaatcaaga gagagttttt
      661 gtatttaagg aggaaatata aatatgttta aaagtttatc atataaattt gcaaatattt
      721 tggttagaaa tgaagttata gaagatgaag attttgaaat atatagatat ggatttgaga
      781 cattagttta ttttatcatt aatatttcag tagcattact tataggtatt gctcttaata
      841 aatttataca aacaattata tttttagtct gttactgtac tttgaggcaa tttacaggtg
      901 gatatcatgc tagaaattat acagaatgta caataacatt tgcactaatt tatatatcaa
      961 ttattttagt tactaagaat atagacattt ataagtttaa atatatatta atactattac
     1021 ttttactaag tacagttata atacataagg ttgcgccctt agagcataga aataaacctc
     1081 ttagtatcta tgagaagaag aattatagaa atattataaa aaagataaca ttatcaataa
     1141 ttatagtagt tacaataagt ttaatattta atattatgag tgaatacatt atatattcct
     1201 cattagctgt atttttaatt acaatattac tattagtaca aattattatt aatttcttca
     1261 aaaaataatc tatttagcta agcttaatgt ccagttagac atagtatatt gaatctaaaa
     1321 cttgagggtg atattataat agcaagttag atattaaaaa agtaatttta ttagatattt
     1381 gta
//
LOCUS       DAEMAE010000394         1382 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEMAE010000394 DAEMAE010000000
VERSION     DAEMAE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.401"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(78..1346)
                     /locus_tag="KPH93_003737"
     CDS             complement(78..1346)
                     /locus_tag="KPH93_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690466.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIINKNGVRNDTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEENTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYD
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGVVDIVGTKPVKFQV
                     FDMKLKRNVYEAELINGDISLQEKLFFVAN"
BASE COUNT          461 a          221 c           95 g          605 t
ORIGIN      
        1 tattttgaga cactccctta cttatattta tctgtaatgc atataaaagt ttaaaattct
       61 aaaattaatt ctatcattta atttgcaaca aaaaataact tttcttgtaa acttatatct
      121 ccatttatta gctcagcttc ataaacattc cttttaagtt tcatatcaaa tacttgaaat
      181 tttactggct tagttccgac tatatcaaca acccctttaa atccttttaa ttctcctgat
      241 atactgttct taatacctga aatagaatat atcttatcat caagtaactt aactttccta
      301 actccagatg atgcttcatt ataatctact tttaaatcaa gtatatcata atcttctaat
      361 ttaatctcat tagaattaat atcataaaca agattggtta tggaattgct catatctgta
      421 actataagat tcaatttatt attttttacc atataatcta gcttagtttt acttaataag
      481 ccaatctcct ttggatttgc attcttttct ttgtttagat tatccataaa tttattactt
      541 atattaaaag tgttctcttc tatatcatat ataataaggt tagagtaact atcatgtcct
      601 tcaatatctc catttttact tttattattt actatttcta agtataattt acttccaata
      661 gaaaatttaa tttcattgat attttgttga tatccattct gtaatcctaa attaaatcta
      721 tttaataatt tataagactt attttttata tctatcttta atatacaaat attattgacc
      781 ttagaacctg aaatattagt ttcactaatt accatatata aattgccttt atatatttta
      841 ttaaacaagg ttttccaact attttcttta aaatcagatt tatttaaatt aatatcaaaa
      901 ttttcttcta ccccactttt taaattttta tttgaaacct ttatgcaatc atttaaagca
      961 ttcattcctt gtactaaatc agacttaaca gaagtatacg cttctcctat attcaaatca
     1021 tctttatata taagttcttt tcctttatca ttaacaaaat cactgtattt tttgtcaata
     1081 tttaaataat catttctaat tcttgatttt gggtttgtat catttcttac tccattttta
     1141 tttataatta cctcctcata agaaaatttg cttatattag gtgtatatac tacacttact
     1201 ctccctatat cttttacatt gccttttaaa gtttttacag atagtttatc cttattaaaa
     1261 taactcaata ttaaggtcat agtaatcatt aaaaaacata ctattataaa acatattgta
     1321 tattttccta tattttttgc tttcataata tccctcctaa aagtcaatct tttgattcaa
     1381 aa
//
LOCUS       DAEMAE010000395         1338 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEMAE010000395 DAEMAE010000000
VERSION     DAEMAE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.402"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1338
                     /locus_tag="KPH93_003738"
     rRNA            <1..>1338
                     /locus_tag="KPH93_003738"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          357 a          292 c          397 g          292 t
ORIGIN      
        1 gctgggatgt gatggggagc gaaatttagt agcgaagtag ctgatttcac actgtcaaga
       61 aaagtctcta tcgaggttaa aggtacccgt accgcaaacc gacacaggtg ggtgaggaga
      121 gtatcctaag gccagcgaga gaactgttgt taaggaactc ggcaaaatga ccccgtaact
      181 tagggataag gggtgccacc atccggtggc cgcagagaat aggcccaagc gactgtttac
      241 caaaaacata ggtttctgct aagtcgcaag acgatgtata ggagctgacg cctgcccggt
      301 gctggaaggt taaggggatc tgttagagca atcgaagcag tgaacttaag ccccagtaaa
      361 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      421 ccgcacgaaa ggcgtaacga tttgggcact gtctcaacaa cagactcggt gaaattgtaa
      481 ttccggtgaa gatgccggat acctgcgaca ggacggaaag accccatgga gctttactgt
      541 agcttgacat tgggtcttgg tactacatgt acaggatagg tgggaggctt tgaaaccagg
      601 acgccagttt tggcggagcc atccttggga taccaccctt gtagtactgg gactctaacc
      661 ataggccatg aatctggtct tgggacactg tcaggtgggc agtttgactg gggcggtcgc
      721 ctcccaaaag gtaacggagg cgctcaaagg ttctctcagt acggtcggaa atcgtacgta
      781 gagtgtaaag gcaaaagaga gcttgattgc aagacataca ggtcgagcaa ggatgaaaat
      841 cggacttagt gatccggtgg ttctgcgtgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcatcacatc ctggggctgt agtaggtccc aagggttggg ctgttcgccc
     1021 attaaagtgg tacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcaggcgtag gaaatttgag aagacctgtc cttagtacga gaggaccggg atggacgtac
     1141 ctctggtgta ccagttgttc tgccaagggc atggctgggt agctatgtac ggaatggata
     1201 agcgctgaaa gcatctaagc gcgaagccaa cttcaagata agatttccca ccgcaagggt
     1261 aagaccccag aaagactatc tggttgatag gtcgaaggtg taagtgcagc aatgtattta
     1321 gcttatcgat actaatag
//
LOCUS       DAEMAE010000396         1336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEMAE010000396 DAEMAE010000000
VERSION     DAEMAE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.403"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..1159
                     /locus_tag="KPH93_003739"
     CDS             170..1159
                     /locus_tag="KPH93_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG1690467.1"
                     /translation="MLKEEKTYQEKEAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLIFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAASRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVSEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          509 a          130 c          246 g          451 t
ORIGIN      
        1 gaaatcaata ttaatacatt aaaataacta aatacatgta tgcataaaga tgtatggaaa
       61 atatataaac atatagtatt aatttcaaat tcaacaattt aagtgttaaa agtgttatta
      121 ttaaattaga aatatcttaa cttaatatac tgagatggga gtgatagata tgttaaaaga
      181 agaaaaaact tatcaagaga aagaagcaaa agtattcaat ggagtagcgg ctttgataat
      241 gattgtatta tttttagtag tttcaatagg tatcatggtt tttgggatag taaaacttga
      301 tagtggattt actggaatag gttcacttat gttaattttg ggacttatat ttatagtagt
      361 agattttatc ttattctttg ggctaaaaat gattaatcct aaagaagcta tagttctagt
      421 tttgtttggt aactattatg gaactataaa aaaagaaggg tactattggg tgaatccatt
      481 ttgttcagca ataaacccag cagcatcaag aattaatgta tcgaaatctt catctgatga
      541 aaaggtagat atatcaacta attcaagggg caaaaaagta tctttaaaaa caatgactct
      601 taataatgaa aagcagaaag tcaatgattt attgggaaat ccaattataa taggagtggt
      661 tgtaatttgg aaggttatag atgcaacaaa ggcagtattt aatgtggata attataatac
      721 attcttatct attcagtgtg attctactat tcgtaatgtt tcaagacttt atccatatga
      781 tgtatctgaa gatggagatg aaaaatctct tcgtggtagt agtcaggaaa tagcagatag
      841 attaaaagat gaattacaat caagagttga cattgcagga attgaagtga gtgaagtgag
      901 gattactcat ctttcttacg caccagagat tgcagcagca atgttacaac gtcaacaagc
      961 agaggctata atagcagcac gtaaaaagat tgttgagggg gctgtaagta tggtagagat
     1021 ggcattgact aatttaagtg aaaataatgt tgtgactttg gatgaagaac gtaaagcagc
     1081 tatggtaagt aatctgttag ttgtgctttg tggcaataaa gatgcacaac cagtagtaaa
     1141 cagcagttct atttattaaa ttatagaaaa tttaatagtt tattctattg atatatagaa
     1201 aatctagcag ttcattttat taaattatag aaagattaat ggttcacttt attgaattat
     1261 aaaaagttta ataatttatt ctattgatat atagaaaatt tagtagttct atttattaaa
     1321 ttataaaaag tttaat
//
LOCUS       DAEMAE010000397         1322 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEMAE010000397 DAEMAE010000000
VERSION     DAEMAE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.404"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(104..1120)
                     /locus_tag="KPH93_003740"
     CDS             complement(104..1120)
                     /locus_tag="KPH93_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG1690468.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGNVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWN"
BASE COUNT          475 a          265 c          136 g          446 t
ORIGIN      
        1 gtatagtaat atttgagatt ttaattatca ctattttttg taagaataca caatttatat
       61 ttaaatacct gacagaatat ttaaatataa atttgtttta aaactaattc catataactc
      121 taccatctat atccttaaca ttttgacaac cagtcaaaat catagtctca cataactcat
      181 tcctaacctt ctctataaaa aactccacac catcaagacc accgccaaaa gaagcagtaa
      241 caaaaggtct tcccatcaaa acagcatcag cacccaatcc aagcatctta actacatcaa
      301 caccagtcct aactccacca tcaacaagta ttgttatctt tccctttaca gacttagcta
      361 tatctggaag tacctcacaa gtaccaggag tataatcaag tactcttcca ccatgatttg
      421 aaacaatgat ggcactagca ccagactcta cagccatcaa agcatcatct acagtcataa
      481 ttccttttat aataaaaggt aatctagtag attcaaccaa ttctctaatc tcatcaattg
      541 tcttaggaga aaaaggattt tcttgaaatt gattattaat aagtccacaa gcatcaaggt
      601 ctacaccaac tgcaaaagca cctgcttctt cagacaatct aattttttct attatctttg
      661 aattattcca aggctttata aatacaattc catttccact attatctttc aacacatcta
      721 aattatctag taaaaaagta tctacattag tatcgccaac catagcataa attccagaat
      781 tagaacaacc tctaacaaca ggctctatgt attctttttc agaaacttta ccacccatgt
      841 tcaatagagt acctgataca ggtgcagcga atataggaga actcattttt ctaccaaata
      901 attctatgga tgtatctggc tttgaaacat tatgtatgac tctcatattt atctttattt
      961 tttctagact ctttctattt tcaatgaaag acgaaccact tcctttacca cccataccag
     1021 gaacatttcc agcacaagct aatccattac aaattttaca tactttacat gttccattaa
     1081 agttctctct agctgatttt agtaagtcat tgtaattcat acaaaatctc tccttttata
     1141 atattaaatt tgagtattac taaattgaaa atatcttaat tgtaaatata gagatgatgt
     1201 tatgatgagc taaactagat aatacatatt agatattttt tattgttgta gatagttaaa
     1261 ttatatcata ttagatagtc aaattttgaa aaaaactaac ttatttaagt atacattttg
     1321 aa
//
LOCUS       DAEMAE010000398         1308 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEMAE010000398 DAEMAE010000000
VERSION     DAEMAE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.405"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            112..765
                     /locus_tag="KPH93_003741"
     CDS             112..765
                     /locus_tag="KPH93_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG1690469.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVSLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(923..1099)
                     /locus_tag="KPH93_003742"
     CDS             complement(923..1099)
                     /locus_tag="KPH93_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690470.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT          464 a          154 c          159 g          531 t
ORIGIN      
        1 tattaagact tgtaaaaaaa acataaagtg gtataattaa atatataata tagtaatgaa
       61 aactatattt atgaatctga tttttaaaat acataattgt aggaggacga aatgtatcaa
      121 ttttttttaa agggtagaga cccaataagc agtttaactc attttatagg tgcttgttta
      181 tctcttttag caactataat tttagttttt caatctgttt cattacagga aacttctctc
      241 ttgatgatag tatctgtatc ggtatttgga ctttcactaa ttgctttata tagtgcaagt
      301 tcgtattatc attttttaaa ggggacacca gaacaagagt tgttttttag aaaagtagac
      361 catgcaatga tttatgtatt gattgctggc tcttatacac caatatgtct gaattttatg
      421 gaaaaaaaag aaggcataat atttgttaca gcaatatgga tagttgcatt tattggcata
      481 ataataaaaa tattttggat ggatgcacct aggtggttat ctaccagtat atatttactt
      541 atgggttggg caattgtttt tgatataaat gcattcaatt caattcctaa ggattgcctt
      601 agactattaa ttatggaagg tgtatcttac tctataggtg caattattta tataattaaa
      661 aagcctaata taagtcctga atttggattt catgaaatat ttcatatttt tattatgata
      721 ggttctttat ttcacttttt agcagtttta ttgtatgttt tatagtttta aaagatttga
      781 ttaattgata ctaaaattct acaatgaata aaatggaact gccttatata gattatatct
      841 tcgacaataa gacagttcct tattataaat attaaattct acttataata aaattttgat
      901 aaaaatagtc cattcaaaat gcctattcca taattccttt aatgtacttt tcatttacga
      961 catatagttt gaatttatgt actataatta tatcctctaa attctctgaa acactaacaa
     1021 ttctgacaca tttgtttcta atttctacag gtaaatctaa atcatttgtt aaattcacat
     1081 agtcatcata tttatacata aaatcatccc tttacattaa gatatactta ttatactata
     1141 ttttaataag tttttttaaa ttattacaat ttttcccgaa atttcatttt ttatacataa
     1201 aatttccaaa ttaggtaaag attaaatttt ttattgtata aacaaaagtt acatataaaa
     1261 tattttattc aaatttttaa ataactattg ccaatttttg aaaaatat
//
LOCUS       DAEMAE010000399         1286 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEMAE010000399 DAEMAE010000000
VERSION     DAEMAE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.406"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1..265)
                     /locus_tag="KPH93_003743"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="KPH93_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            472..>1286
                     /locus_tag="KPH93_003744"
     CDS             472..>1286
                     /locus_tag="KPH93_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1690471.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRISITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVS"
BASE COUNT          548 a          123 c          180 g          435 t
ORIGIN      
        1 aataatatga tgctattgtt ataatagata ctaatgttaa tgatgttatt gcagtatcta
       61 atcttattat ttaataaact catttctata tattcaagtt ctattaaata ttgataaaat
      121 atttctagtt taacagcttt ttctctatct atatttcata ctttaactct aatttatcta
      181 aggctttttt attacaataa ttaactttat gtttaattaa atatcttatt gtagcaccta
      241 ttacaacttc cactctaaca agcataaata taatattcca tccaaaagtt aagagggtat
      301 atctttttta tgatgagaat ggataaaata tttataactg acatctaact gacatttaac
      361 ctttaaaatc aaaaccatat tgattttcta aaataataac tacttgattt agtcaataat
      421 aaagaatata gtaactatat agacaacaaa aagaaaagga gtgcataacc cttggaaaaa
      481 cagtatttta caacaggtga atttgctaaa ttgtgtggta tatctaagca gacattaatt
      541 ttctatgata aaataggtat tttttctcct gaatataaag ataaaaataa ttatagatat
      601 tactcagttt atcaatatga tacacttgat atattacaat cattaagaga aataggaatg
      661 agtttagagg aaataaaaga gtatatacaa aatagaactc cccagctttg tgtgaaaatg
      721 cttaaagaag aagaaaaaaa gataagagaa aaaataaaaa aactaagaaa gataagttct
      781 aagatgcaaa atagaataag tataacagtt gaaggaattt ctaaaatgaa tagtgaagaa
      841 atttatatat ccaaaaagtt tgaagaatat ttagttctat caagcgattt gagtaatatg
      901 agtaatagtg attttatgat ggaattaata gatttcacca attattgtaa atcgaaaaat
      961 ttgtatcaag gatatgagtt gggagtaata gtaagtaatg aaaatataaa aaatggggac
     1021 tatttaagta tatcaagttt ttatttgaag atagataaaa aaataaagga taaaaagcta
     1081 tatgtaaagc cagaaggaat gtatgcttgc ataaaacata ttggaaaata tgaggattca
     1141 tataagtctt acgaaaaatt gaaaaaatat atatatgaaa atgaatataa aattattgga
     1201 aattcgtatg aggagagtat acttgacttt ttttgtgaaa gtaatgaaga taattatatg
     1261 actgaaattt caatccaagt atccag
//
LOCUS       DAEMAE010000400         1286 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEMAE010000400 DAEMAE010000000
VERSION     DAEMAE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.407"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..1050
                     /locus_tag="KPH93_003745"
     CDS             370..1050
                     /locus_tag="KPH93_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1690472.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVNAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          575 a           80 c          191 g          440 t
ORIGIN      
        1 atgcgcgtag acaataatgt ggatattatc aaatttatct ttatattaaa ttgttaaaga
       61 cataattatg agaataaaaa atatagcttt gaattatata attgtatatg cttatcttta
      121 tacagtaaat aaaaaatcaa atgaaatact aaataaaatg tatatcttaa aataacttac
      181 tagatataca agcatgttat gtgaattttg aagtgtgcag tttagataat aaatatatac
      241 aaataataaa gaaaatggat ttataggatt taaaatttct tataagggta aaaaatgtta
      301 ttagaaaatg tactaaaaat atttttccaa ttctttatag aaaaataatg atatttagga
      361 gagattgtaa tgcaaattga tgtattgata attgatggag ataagaatag ttctcaagag
      421 ctgaaaaatt ttttagaagg aaaagggata aatgtctatt tagcatataa ttgtaaagat
      481 gctattataa aaatatttag caagaaattt gacttaatat ttttagagat aattttggtt
      541 gatggtgatg gttgggattt ttgtaaaaaa attagaaata taactacgtg cccaattatt
      601 catataactt atataaatga aaatcaaagt attttaaatg ctttaaattc tggtggagat
      661 gattatttaa taaagccttt aaatttagaa atattatatg cgaaagtgaa tgctatttta
      721 agaagaagag atttattttt aaataataat aaaaataata taaaactaga agattataat
      781 agagctagtg gagtaataaa gttagaaaat aaaatgataa agttaactcc aacagaaaat
      841 agattattaa attactttat agagaatgct gaaaagactc taactattaa agaaatatat
      901 gaacatgtat ggatgaatga gtatttagat gataattata gtgtagtggt tgctgtgagt
      961 ggattaagaa agaaaataga gaaagattat aaaaatcctc aaaaaattat aacaataaga
     1021 gaagtagggt attactttaa caagatataa agctttataa atagttttat aaatagaatg
     1081 aatgataagg catagataat atgttagtat attacaagag tttctgtcct aacgatatat
     1141 ttgtaaaatt agatttagtt tacttaatta aattatagca gtagaataag tataaaaaat
     1201 gtctgaaata ttatagacat cagtaaaata tataaaacta taatataagt tgtaataata
     1261 aaacaaatga atattaatta ataaaa
//
LOCUS       DAEMAE010000401         1280 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEMAE010000401 DAEMAE010000000
VERSION     DAEMAE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.408"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            362..1183
                     /gene="yidA"
                     /locus_tag="KPH93_003746"
     CDS             362..1183
                     /gene="yidA"
                     /locus_tag="KPH93_003746"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG1690473.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT          525 a          167 c          170 g          418 t
ORIGIN      
        1 atatgattaa tggaattaca atattttata acacaaaact aactaatata acaaattacc
       61 atcattctat ttattcaaac ttctaactat ataatataca tttatttata ttaacaatgc
      121 aaacgatacc attatttgtg attaagaatt ttggaattat tgattacaaa gtttttacat
      181 tcttatctca cataagtaat atttttatca aaaaattaat attattaaac tttctaaatt
      241 acttatctct tgatataata gtatacaaaa caaaccatat ttcccactga aactttgttc
      301 aacactagtc gtgtcaacgt tttcacagga aatttgagta tataatttat tggaggtcga
      361 tatgtacaaa ttaatcgcac tagacattga tggaacaata ttaaatacac aaaaaaggat
      421 tactcctgaa gtctttgaat ccattcaaga ggcaaaaagg gctggggcaa aagtagttat
      481 aacaacaggc agacctcttc ctggtgtaaa agaacttcta aatcagctaa atttaacaga
      541 tgaaggtgat tatgttatat gctttaatgg agcaattatt caagaagtta aaagtgagaa
      601 aattatacat gatgtagaaa tgactttaga tgattttgat tttatatata ataacgtttg
      661 taaaaaatat aagactaaaa tacacataaa tacaatgaca aatttaatta caccaaatga
      721 aacgcctggc aagtatacac ttcatgaagc aaaattaaat aatatagaag tcaaatatat
      781 acaaaaagat aaaattgatg aatctattaa aatctgtaaa attatgatag ttgatgaacc
      841 agaaagattg gaagaaatca tacaacagct acctaaaaat ttattcaata aatatactat
      901 agtaaggtct gctccttttt atctagaatt tttaggtaaa actactaata aaggaactgc
      961 tcttaaaaca ttatgtacga atctaaatat acctatagaa aatgctattg ctgttggcga
     1021 tgaagaaaat gaccaacata tgataaagta tgcaggtctt ggtgtggcta tggggaatgc
     1081 acgtaatagt ataaaggaaa ttgcagatta tgttactgat actaataatg aaaatggagt
     1141 tgcaaaggtt ataaataaat atatacttaa taaggctatc taatcatcta aaaaaactaa
     1201 aaatatacct ttagatttaa atacttttca catccctact ctatactaat ttatatagta
     1261 gggatattgc ctatcatttt
//
LOCUS       DAEMAE010000402         1278 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEMAE010000402 DAEMAE010000000
VERSION     DAEMAE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.409"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            346..1059
                     /gene="rgbR"
                     /locus_tag="KPH93_003747"
     CDS             346..1059
                     /gene="rgbR"
                     /locus_tag="KPH93_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG1690474.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELKELKKHVLACIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLSKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTVILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT          553 a           90 c          186 g          449 t
ORIGIN      
        1 ctgctattgt actacagatt atattgttga ttttatatgc agtacaatta tataggcgat
       61 taatgtatta tttaaggctt aaaaatttta aataaataaa agaaggtgtc ctaaaattat
      121 ttttttaata ttttaagaca tcttctttta tcagtaagta ctatttttaa ttatagtaat
      181 ttctgtcttt aaattgacat atcaaaataa cagttatatg ggattgagta ttgataaaaa
      241 caatttgaaa atgttatcct aaagtatcag atatacaaaa ttatctttaa atatattata
      301 aatattttaa ttgtattaac tagaaattat gtaaggaggc attttatgat taagatagca
      361 gtttgtgaag atgaaaaaga aacacaactt ttaatagaag attaccttga gaatatatta
      421 aaagatataa gtatagaata tgagatacaa aagtatatat ctggagaaga gttactggaa
      481 agtaatttaa aagatataga tatattactt cttgatataa aaatggaaaa actaaatgga
      541 atggatactg caagaaagat tagagaagta gacaatgaaa tggaaataat atttgtaact
      601 tcactaatag attatgtgca agaaggttat gaggtaagag cttataggta tttactaaag
      661 ccaatagagt tgaaagaact aaaaaaacat gtgctagctt gtatcaaaga tattgaaata
      721 aataaggaaa gtcatattac aataaaaaat aagtctaata catataagat ttatttaaat
      781 gaaataaaat atatagaagt tcaaaaaaaa gatatgctaa tacacacaat aaacaagaat
      841 tttgatataa aatatagttt aagtaaaata gaaaaagaat taaatccata taaatttata
      901 agatgccata aaagttttat agtaaattta agatatgttg aaaatataaa gcctaatact
      961 gtgatattgg aaagtggaga agaggtacct attagtagat atagatataa agaagttaag
     1021 gagaaatttt taaaatttct tggtgataca atatgctaaa ttttatgact gagctattag
     1081 tagttttatc tatatttata cagttttcta taatgaacgg aattttaatt tataaaaagg
     1141 atagtaaaaa aaatgaaaaa atagtatttt atattgtttt gtttcttatt tacattatag
     1201 ttcagataag tagattattt aatggaggaa attactttag taatataaac tatttatttt
     1261 atatattagc atttattg
//
LOCUS       DAEMAE010000403         1272 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEMAE010000403 DAEMAE010000000
VERSION     DAEMAE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.410"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..363
                     /locus_tag="KPH93_003748"
                     /pseudo
     CDS             1..363
                     /locus_tag="KPH93_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(337..510)
                     /locus_tag="KPH93_003749"
     CDS             complement(337..510)
                     /locus_tag="KPH93_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690475.1"
                     /translation="MNDATKVSIKNETNNIMNLCNLLSWNALLQNNKINAETIAKSRY
                     FIIILSPIDFYRL"
     gene            651..969
                     /locus_tag="KPH93_003750"
                     /pseudo
     CDS             651..969
                     /locus_tag="KPH93_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          511 a          136 c          171 g          454 t
ORIGIN      
        1 tatacaataa caatatataa gctaattaaa aaccttaaga agaacgatat tgacactact
       61 tgaattatat tatagaaaag ctgaaataaa taaaatagtt tgggctagaa taggttttaa
      121 tttatatctt tctaaaggag ttgcattaac tttaactgat gctggtatag ggatttcagg
      181 acttttggct ggtgctattc ctggtatagg acagtacttg ccacttatag ttagagtaat
      241 tacaatgtac cttgagtcaa atataacatc tggaaaaatc tttaaattta aaaaatacgt
      301 tataataaag gaggtgcttg tgtaaccaaa tactacttac aatctgtaga aatcaatagg
      361 tgataaaatg attataaaat atctactttt tgcaattgtt tcagcattaa ttttattatt
      421 ttgtaataaa gcattccaac taagtaaatt acacaagttc attatattgt ttgtttcatt
      481 ttttatacta acttttgtag catcattcat tagtagataa aaaataagat ggtggggtta
      541 acttaccatc tttttctcat ttaatatttg taatatttta gatactcttt atacttgtgt
      601 tactgttata ctttgattag gaaaagtaaa tttaacagga ggtatctgct atggattttg
      661 gaaatatcaa tcttatactt atcggaataa tagttataat aggaactaca ataatatatt
      721 taattaaacc taaaactgct ttttgtagta aaaaatattt taataaatta gaatcaatat
      781 atggaaatat agataagaaa aaaactgtta aactagaaat actttatcgc tatgtaacag
      841 gtcttgagta tatagcaata ggcttattta caaaaagatt agatattaca aataatatca
      901 ataatattag tagctactat aacagtaata ttatattact tagttagaaa gagatatata
      961 actatataaa atctaataga attaatatca aaagaaggta gcaactaatc accagtaatg
     1021 taaaataatt tgtttttcaa agttaataaa agtcttcttt atggcaatga tttaaatata
     1081 atcaaatatc gtaacggaga tttttttatg aataatttaa gaaaacaatt aatcaaagaa
     1141 attatataag atggagaact tataaaatct acagatataa gtagttttag gagtaaacac
     1201 agatgactta tttcaaagag acaatagcta caagttatcc tcattctgta atacaaaaat
     1261 gtttaataca tt
//
LOCUS       DAEMAE010000404         1260 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEMAE010000404 DAEMAE010000000
VERSION     DAEMAE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.411"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..75
                     /locus_tag="KPH93_003751"
     tRNA            1..75
                     /locus_tag="KPH93_003751"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Thr,seq:tgt)
     gene            90..174
                     /locus_tag="KPH93_003752"
     tRNA            90..174
                     /locus_tag="KPH93_003752"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Tyr,seq:gta)
     gene            185..268
                     /locus_tag="KPH93_003753"
     tRNA            185..268
                     /locus_tag="KPH93_003753"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Leu,seq:tag)
     gene            297..373
                     /locus_tag="KPH93_003754"
     tRNA            297..373
                     /locus_tag="KPH93_003754"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:331..333,aa:Arg,seq:tct)
     gene            381..456
                     /locus_tag="KPH93_003755"
     tRNA            381..456
                     /locus_tag="KPH93_003755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:414..416,aa:Gln,seq:ttg)
     gene            546..634
                     /locus_tag="KPH93_003756"
     tRNA            546..634
                     /locus_tag="KPH93_003756"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Ser,seq:tga)
     gene            638..713
                     /locus_tag="KPH93_003757"
     tRNA            638..713
                     /locus_tag="KPH93_003757"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:671..673,aa:Phe,seq:gaa)
     gene            720..793
                     /locus_tag="KPH93_003758"
     tRNA            720..793
                     /locus_tag="KPH93_003758"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:754..756,aa:Met,seq:cat)
     gene            808..884
                     /locus_tag="KPH93_003759"
     tRNA            808..884
                     /locus_tag="KPH93_003759"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:842..844,aa:Ile,seq:cat)
     gene            914..990
                     /locus_tag="KPH93_003760"
     tRNA            914..990
                     /locus_tag="KPH93_003760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:948..950,aa:Pro,seq:tgg)
     gene            998..1074
                     /locus_tag="KPH93_003761"
     tRNA            998..1074
                     /locus_tag="KPH93_003761"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1032..1034,aa:His,seq:gtg)
     gene            1083..1158
                     /locus_tag="KPH93_003762"
     tRNA            1083..1158
                     /locus_tag="KPH93_003762"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1116..1118,aa:Lys,seq:ttt)
     gene            1166..1239
                     /locus_tag="KPH93_003763"
     tRNA            1166..1239
                     /locus_tag="KPH93_003763"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1198..1200,aa:Cys,seq:gca)
BASE COUNT          309 a          281 c          330 g          340 t
ORIGIN      
        1 gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta ggttcgattc
       61 ctatcaccag ctccatgtaa aattaatacg gaggatttcc cgagcggcca aagggggcag
      121 actgtaaatc tgttgtcatc gacttcggtg gttcgaatcc accatcctcc accaaaaaaa
      181 atgcgcggat gtggcggaat tggcagacgc actagactta ggatctagcg ccattggcgt
      241 gggggttcga ctcccttcat ccgcaccatt tttatttgat atacatatat aattgtgggt
      301 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      361 tgtgcgctca ccacatatat tggggattag ccaagtcggt aaggcacatg actttgactc
      421 atgtatgcgt aggttcgagt cctgcatccc cagccaaatg atgtatttac taagctatat
      481 ttgaattatt tttattttaa atatagttag gtttaatttt agaataagtt taagtatccg
      541 aatgtggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
      601 ggggccgtgg gttcgaatcc caccctctcc gccaaatgcc cagatagctc agtcggtaga
      661 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaaaatatg
      721 gcggtatagc ttagttggct agagcgttcg gttcataccc gaaaggtcat aggttcgact
      781 cctattaccg ctactaaaat ttaatgtggg ccattagctc agttggttag agcgcccggc
      841 tcataaccgg taggtctggg gttcgagtcc ctgatggccc accacattaa attaattaat
      901 ataaatataa attcgaggtg tagcgcagtt tggtagcgca catggtttgg gaccatgggg
      961 ccgggggttc gagtcccttc acctcgacca atataaggtg ggtatagctc agttggttag
     1021 agcgccagat tgtggctctg gaggtcgtga gttcgactct cattatccac cccaaattat
     1081 atggtctatt agctcagtcg gtagagcacc tgacttttaa tcagggtgtc ccgcgttcga
     1141 gtcgcggata gatcaccaaa aaggtggcga catagccaag tggtaaggca gtggactgca
     1201 actccttgat ccccagttcg aatctgggtg tcgcctccaa aatattccaa ggtagctcaa
//
LOCUS       DAEMAE010000405         1249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEMAE010000405 DAEMAE010000000
VERSION     DAEMAE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.412"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            344..1204
                     /locus_tag="KPH93_003764"
     CDS             344..1204
                     /locus_tag="KPH93_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1690476.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT          482 a          145 c          193 g          429 t
ORIGIN      
        1 aataaatcct tttgataata tatacaaagt aagataaaat tttccattaa ataccttata
       61 attatacaca aaatttttat atgcgttaaa tagtatagag aaatttttca tagaaccatc
      121 tttatagggg atactttata aaagaattaa aaaattataa tatataaaaa acttcatagg
      181 agtgtaacaa catcagtaaa aatatgaaaa cactatataa aaattcaaaa atattaagaa
      241 taatgaaata cttgaaattg cttatatcaa gaaattttat ataatataaa ctggtcaaat
      301 atgttataat attaaattgt gacaagttca tggaggtgtt tatatgaaaa aacaaaagtc
      361 gccaatttta cttgaaatgc ttggactatt tttgggttgt atatcaatga gtattggtat
      421 aaatatgttt ttaaaaccac atacaattgc tcctggagga cttagtggtc tatcactagt
      481 attaaacaag gttacaggac ttccagtatc agctatcatg ttaattatag gtgtcccatt
      541 agtaatactt gcttttagaa ttatgggtac aaaaaattca ttaaaaactt tatttggaac
      601 agttgtattt tcagctatag tccaattaac agacccatta tcaaagctaa gatttactaa
      661 tgacttatta ttgtcttcta ttgcaggagg aatcctagtt ggtataggtt taggaataat
      721 gtttaaatca gatgcttcta ctggaggaac agacttaatt gcactcatac ttagtaagaa
      781 attcccagga ataaaagcta caaaattcat gtcatgccta gatggaatgg ttgttatatc
      841 ttctggtata gtaaatcgta gtttagaaac tgcactttac tctggtatag cactttgtgt
      901 attaataaaa atagcagata tgataatgga aggatttgac cattcaaaag cattttttat
      961 tatttctgat gagcctgaaa gtttaagaca agctataact gaagaacttg atagaggtct
     1021 tactattctt gatggtaaag gtggatatac tagagaaaat aaagaagttc tattagtcgt
     1081 agtttctaaa aaacaagaac tttatctaaa gagattagtt aaaagaactg accctactgc
     1141 atttgttatt gttacagatg tgcatgaggt tcttggagaa ggatttaaaa atttagagaa
     1201 ctaataaaaa attaatattt taagtatata tactatttta attaaaata
//
LOCUS       DAEMAE010000406         1198 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEMAE010000406 DAEMAE010000000
VERSION     DAEMAE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.413"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..1005
                     /locus_tag="KPH93_003765"
     CDS             121..1005
                     /locus_tag="KPH93_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG1690477.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFAARVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
BASE COUNT          455 a          105 c          216 g          422 t
ORIGIN      
        1 atgtataatt ggaatataaa tcttgtttac tatgtacaaa tggtattgta ttatattaaa
       61 tagaaaataa gaactttaaa ctataatatt ggttctaaaa tataaggtaa ggagtttttt
      121 atggatacga cgaaaataga tgatataagc aagttagatt taaataattt agatattaat
      181 aagatggata tgggaggtat aatggaaagt ctattagaat gggctactac aagcggagta
      241 aaacttataa taggtatact tatactttct attggattta aaataataaa aaaatttgta
      301 aaccatgtta tgttattact tagtaaaaga gatattgatt taactttaag aaagttttta
      361 aagtctcttc ttttgagtgt acttaaagtt gctgttataa tcattgttct ggaatattgg
      421 ggaatgagtt tgtccagttt tgctgctgtg atagcatcag caggggtagc aataggtttg
      481 gctttacaag gtagtctatc aaattttgct ggaggattta ttatactatt gattagacct
      541 tttaaagtag gagattacgt agaagcagca ggtcatggag gaacggttga acaaatagga
      601 cttttttata cacaattggt tactccagat aataaacaga tacttatacc taatggttct
      661 gtatcaaatg atagtctaat aaactattca gcaaagaata ctagacgagt tgatttaact
      721 tttagtgtag gatatgaaga tgatatacta catgttagaa gagtacttaa agatattgtt
      781 aataatcata aattaataat aaatgaacca gaaccattta taggagtagt agaacatgga
      841 gataatgcta ttaaatttgc agctagagtt tggtgcaaga cagaagatta ttggacaatt
      901 tatcttgatt tactagaaga ggttaaagtt aggtttgatg aagaaggaat tacgattcca
      961 tatcctaaaa tggatttaac ggtaaaggaa ttaaataaaa tttaattttt taatatagtg
     1021 tttaaattta agaatatcat atagatatgt ttataagatt tgtaaaaaat taataagtac
     1081 tgttattttg taagtaaaaa tatagaaaat gggagtgtct caaaatgaac tttttagttc
     1141 ctgaggcact ctttttgtat gaaatcaaat atatttttat tatttaaaca ataaaaaa
//
LOCUS       DAEMAE010000407         1181 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEMAE010000407 DAEMAE010000000
VERSION     DAEMAE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.414"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            127..>1181
                     /locus_tag="KPH93_003766"
     CDS             127..>1181
                     /locus_tag="KPH93_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG1690478.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEEVQIKDVISIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSC"
BASE COUNT          494 a          108 c          181 g          398 t
ORIGIN      
        1 attttagcag ttttgtattt atttgtattt attttgaatt tatttactaa atttgcctat
       61 aaaaagtata tataaaaata tagttcaaaa tggtataatt aagtataaca gaaggaggta
      121 atgtaagtga gccaatacat aaatataaat tctcagttag acgccctaaa attgaaggag
      181 ttcgagaaaa tattttacga aaatatcaat gaggacgtta aagtagtgcc aaaagttact
      241 ccttttaaag gaataaatac cgatttgttg tatgttaaag atggaagaat tttatttata
      301 aaatttatgg atacaacaga agatatattt tttattttag aagaagagtt acttgaagtt
      361 atgaatgagg aatatgaact tttgaaacta aaaatgggac aaaagaatag aaatataagt
      421 tataactacg tttacattat gccatacgtt gaagttgagg aaacatatga atttgaagaa
      481 tttgttaata ataatataat agataaaaat aagcttcaag atataatgaa taagggttcg
      541 ctggaggaat atcttaatga tgagaatgat gaaataaact taaatttatt tttactagac
      601 atatgttcag aatactatat aataaatgat aaacttcatc taaatgaaaa atttaaaaaa
      661 atttcattct acaatgatga ctataagtat acagcaacta tgatggaaga ggttcaaatt
      721 aaagatgtta tttctattaa atatgggaat acacttattg aaggtggttc tggtataggc
      781 aaaacagcaa ttatgctttc tagggctata aaattagcaa aagtatatcc acatcataaa
      841 ttagtcatat ttacacatac aaaacaattg tgcaatgagc ttcgagagag gatagagtta
      901 ttatacaaag ataataacaa tttagaagtt cacacattta gttcttttat atttaaacta
      961 gctaaaaaat tcaatttaat tgtagattat aacatgttaa aaaatgatta cgaaaagacc
     1021 tttaataatc ttgtaaaaca agcacagaac attataaaaa ataaaaatat gttcaaggct
     1081 atttttattg atgaagcaga aagcttttta gagtatgaaa tagattttat aagggagttt
     1141 ttatataaaa caaaattcat atttaatgtg tgttcttgta a
//
LOCUS       DAEMAE010000408         1140 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEMAE010000408 DAEMAE010000000
VERSION     DAEMAE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.415"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(192..1130)
                     /gene="pdaA"
                     /locus_tag="KPH93_003767"
     CDS             complement(192..1130)
                     /gene="pdaA"
                     /locus_tag="KPH93_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG1690479.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQKKETQLQEDNE
                     KIDSGKDTSNVIVSDGTDKPSKGTANNDNNKLDVSSLDNTALDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDAIYNGPTKDGQKTLYLTFDEGYENGYTTKILNTLKQNQVKAVFFVTY
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLENFNEELYDVEKLYKDVTGKD
                     IVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT          339 a          175 c          142 g          484 t
ORIGIN      
        1 ctgttatcta tatttagtat cttgccaaat tttttgatgt tttattgacc tgtcctacca
       61 acatatataa gctatcacaa gttttttacc tatactatta ccaaacaaaa attctaattt
      121 taatttatta ttttataacc tatattatat taactatact agtttgtaga ctttagttgt
      181 aattctaaat cttaatactc caagagttca aattcatatc ctagttttct tgcatttgat
      241 ataaaatcat ttaaaatttc tgtactagtt ttggaaactg catgtagtaa caagatagaa
      301 ccatcatgta gattatccat tattttttgc gttgcttctt catgtgatgg ttgtttatca
      361 gtatcccagt ctctatatgc aaaactccaa aatacagtct tgtatcctaa atttttagtc
      421 attgccaagc ttttttctga gtattttccc ataggaggcc taaaaaattt aactatatct
      481 ttcccagtta catctttata caacttttca acatcatata attcttcatt aaagttttct
      541 agattacttg tttttgttgg catagatgga tgggttttac tatgatttcc aactatatgt
      601 ccctcagaaa ccattctttt tactaaatct ttattttctt ttatatatgg atatgtaaca
      661 aaaaaaactg ctttaacctg gttttgtttt agggtgttga gtatctttgt agtgtatcca
      721 ttttcataac cttcatcaaa tgtaagatat agtgtttttt gaccatcttt tgtaggtcca
      781 ttgtatatgg catcatagtc agaaaattta aattcaatgt ccgtatttac ttctggtgtt
      841 ttatgcttat tgtttggtat ataaaaccaa tcaagagcag tgttatctaa agaagataca
      901 tctaatttat tattatcatt attagcagtg ccttttgatg gtttatctgt tccgtctgaa
      961 actattacat tggaagtatc cttaccacta tctatttttt cattatcttc ttgtaactgt
     1021 gtttccttct tttgattttc gttttgattg ttttgtgaat ttgaacatcc aacaataaaa
     1081 aaaatagata tagaaagtag tgttaaataa tgtaatactt ttctaaacat tttgaagctc
//
LOCUS       DAEMAE010000409         1139 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEMAE010000409 DAEMAE010000000
VERSION     DAEMAE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.416"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(169..924)
                     /locus_tag="KPH93_003768"
     CDS             complement(169..924)
                     /locus_tag="KPH93_003768"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG1690480.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKADVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLHKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIKEVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLNHH
                     SQSDEVFANGSIIAAKFLIGKQSGLYKMSDILG"
BASE COUNT          393 a          176 c          129 g          441 t
ORIGIN      
        1 aatatgattc catatagtaa taaataagat aatctttaag agaaatgatt atatttaaaa
       61 ggtaagcata aaatttataa acttttagaa tagaaatctc aatcaattag atattcaaat
      121 tgatgaatat gaatacctaa atagattgag atttattatg tacaaaactt atcctaatat
      181 atctgacatt ttataaagtc cagattgctt accaattaaa aatttagctg caattataga
      241 accgtttgca aatacttcat cagattgaga atgatggttt aatgtaagaa cctcattatc
      301 acctgcaaat ataacatcat ggtcgctgat tatattgcca cctcttatag agtgtattcc
      361 aatttcattt ggttttcttt tacaatctct accatatcta ccatgtgtat tttcactttc
      421 aggcaagact tctttaattg cattagctat catagtagca gttccactag gggcgtcttc
      481 ttttctattg tgatgttttt ctatgatttc aatatcaaaa ccttctaaca tctttgaagc
      541 atccttaact aattttatta atatattcac tccaagagat gtatttgatg ataataatat
      601 agggatgttt tttgatgctt cttctatttt atgtaaatca ttttcgtcat atccagtagt
      661 agctaatact acaggtgtat tattttttag agcatatgac aatattggct ctaaagtctc
      721 atgatgtgaa aagtctatta tgacatcagc tttttcagtt acatcagtca ttgttttgta
      781 aagctttgta tcactatgat gacctttttc atcagcagac ctagctacac cacaaaccaa
      841 ctctaattca ttatcttttt ttacacactc tgctaaaact cttcccattg ttccattata
      901 gccactaatt ataactttca acataaattc ctcctatgaa attaataaaa atttgttata
      961 tttctataag tttattttat cactaatata atttatataa aataacatta gtgataataa
     1021 gcttacaatt tattttacat taaaaaaatt taagtggatt tctcaaaata tagataaact
     1081 tctttttcat tgtttaaatg ataaaaatta tttgacttca tataaaaaag agtgtccca
//
LOCUS       DAEMAE010000410         1126 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEMAE010000410 DAEMAE010000000
VERSION     DAEMAE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.417"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            160..>1126
                     /locus_tag="KPH93_003769"
     CDS             160..>1126
                     /locus_tag="KPH93_003769"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG1690481.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDISLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETK"
BASE COUNT          478 a           91 c          200 g          357 t
ORIGIN      
        1 aagaatcagt agatataatt agataatcat aaattaaatt aaaatatttt aatgagatgt
       61 aactttttta gaatgaaaaa ccgtagtata ttgatataat agaaataaga cttaaaaatc
      121 tgtttatagt attatttata agtacagtag gaggataaaa tgtttgaaaa tttacctaag
      181 atagatttac attgccactt agatggaagt gttagagtag aaactatgct tgatatagct
      241 attaaagaga aaatagattt accttcaaat aatatggatg aaataaaaaa attagcaaag
      301 gtttctttta attgtacttc actagatgaa tatttagaaa aatttgattt acccttaaaa
      361 gtaatgcaat ctaaggaaaa tttaaaaaga attacttttg aacttttgga agatgcaagt
      421 agagaaaatg taaaatatat tgaaattaga tttgcaccat tacttcacac ccaaaaaggt
      481 atgagtgtta aaaatataat tgagggtata attgaaggaa ttagagaagc tgaaagtatt
      541 tatgatataa aaggcaattt aatattaggt tgtatgagaa caatgacctc aaaagaggca
      601 ttattagtaa ttgaagaagg aaaatcattt gtaaataaag gtgttgtagc agtagacttg
      661 tgtggtccag agaaagaggg attttgtaag gaatataaag atgtttttaa attagcaaga
      721 gaatatggat ataaagttac aatacatgca ggagaagctg caagtggaga aaatgtattg
      781 gatgctataa atattttaaa ggcggatagg attggtcatg gggtaaaaat taaagaccat
      841 aaaaaggcgt ataatttagt taaagataaa aaaatattgc ttgaattatg tccaactagt
      901 aatgtacaaa ctaagacagt agattcttat gaagcacatc cattttatac tttttataaa
      961 gataatttac atgtaagtat aaatactgat aatagaactg tatcagatat aagtttaagt
     1021 tcagaattaa atgttatatt tgatactttt aaactaggat tagaagacta taaaataatt
     1081 tatagaaatg cagtagaagc tagttttgca gataaagaaa ctaaag
//
LOCUS       DAEMAE010000411         1112 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEMAE010000411 DAEMAE010000000
VERSION     DAEMAE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.418"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            190..894
                     /gene="cwlD"
                     /locus_tag="KPH93_003770"
     CDS             190..894
                     /gene="cwlD"
                     /locus_tag="KPH93_003770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG1690482.1"
                     /translation="MRKYIKHIIFSFAMICLAVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          487 a          114 c          176 g          335 t
ORIGIN      
        1 atttattgta tagtgttgaa tgttagataa acatagtgag ttaaataggg gcaggaatat
       61 aatatcatta cataataata tattaatacc aataaataaa gatatatcaa tattaaaaaa
      121 attaaatgtt tgtactaata tctggaatat tatagtgttt ttataagtat acatatacag
      181 ggtgatgttg tgagaaagta cataaaacat ataattttta gttttgcaat gatatgtcta
      241 gcagtagtat ccatatttga aattaaaaat atttctgaag atgttatcaa gtatatgcca
      301 gtaacaaaca aaactataat tttagatgct ggtcatggag gcattgatcc aggtgcatta
      361 aataaggata agagtacatc tgaaaaggat attaacctag caataacact taagcttaga
      421 gagcttatag aatcaagtgg cggtcttgta atattaactc gagaagatga tagtagcctt
      481 tataaagaag aaaacaataa aactacaagg caaaaatata atgagaattt gaaaaataga
      541 aaagaaataa tatctaattc taacgccaat atgtttgtct ctatacatct taatgcattt
      601 gaacaatcga aatactatgg ggctcaaact ttttatccta aagacaaaca agacagtaaa
      661 gaattatcga agtgtattca agaagaactt aaaagagtag tagataagac aaacaacaga
      721 gaagtcaaac caagggatga tatatatctt ttgaaggaaa ataatattcc atcagtactg
      781 atagaatgtg gttttttatc aaatgaaaaa gagtgtaaac tcttaactga tgaaacatat
      841 caagaaaaaa tagcatgggc aatctacata ggaatacaaa aatatttaag ttgaaataac
      901 tataaaaata ggtctaaata tgtaaatagc ttgtattaaa gcagttaaaa tgcattaata
      961 taggctattt ttattttgac aaaaaaatat ttaaaataaa agttaaaaaa ttgttgactt
     1021 agaataatat agatgatatt atatatgagt gcccaaaagg agcaccaaaa taagacaaaa
     1081 gaactttgaa aattaaacag taggttaatt ta
//
LOCUS       DAEMAE010000412         1064 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEMAE010000412 DAEMAE010000000
VERSION     DAEMAE010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.420"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            70..303
                     /locus_tag="KPH93_003771"
     CDS             70..303
                     /locus_tag="KPH93_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG1690483.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFILTKW"
     gene            499..927
                     /locus_tag="KPH93_003772"
     CDS             499..927
                     /locus_tag="KPH93_003772"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG1690484.1"
                     /translation="MIRKLNNKDINKIMEIWEKSTIKAHDFISKEYWQDNYNAVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNIGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT          488 a           89 c          158 g          329 t
ORIGIN      
        1 aataataaaa atatcaatta atgttattag ggggggttac tcattagtaa attagctaat
       61 acacttagaa tgatgatatt aattcaaagc agaggcaaaa tgaaatgcaa agaattatca
      121 gaagagttgg aagtaagtga aaggcaaata aaaagttata aaatgtactt agagcaagct
      181 ggtatattta taaattcaac acctgggatt tatggtggtt atgaaataga taaatgtaat
      241 agcatctcat taattaaatt attagatagt gaagtttcaa tttttatatt aactaagtgg
      301 tagataagta gaatattagc aatatcaacg agtatttaga aacttgtttt atattaataa
      361 agcagtatat aaaattgtag aatcaacaat agcatatact aaaacatcaa ccttatatta
      421 actaaatcat atctaaataa aatacactta cctataaaca ttataaaatc aatacaaaaa
      481 tgaggtgaaa caaaatttat gataaggaaa ttaaacaata aagacataaa taaaataatg
      541 gaaatatggg aaaaaagtac aatcaaagca catgacttta taagtaaaga atactggcaa
      601 gataactata atgctgttaa aaacgaatat atacctatat cagatacatt tgtatatgat
      661 gatggagatg aaataaaagg atttataagc ataatagata aaagctttat aggagcttta
      721 tttataaaac ccaaatacca aaatataggt atcggaggta aacttttaga ttatgcaact
      781 aaaaaatata aaagtctaag cttagcagta tataaagata ataaaaaagc agttgtgttt
      841 tataataaaa aaggttttaa tatagtaaaa gaacaagtaa atgaagattc aggatttaaa
      901 gagtacataa tggaatatag taaataatat gagtacattc tattaaaagt ataattaatt
      961 tatttgccat ttgattagga gaatttttat aagagtaatt tttaaaatag actatatgaa
     1021 gataaagata tttattatag atgtaagtta atcagaattg atat
//
LOCUS       DAEMAE010000413         1039 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEMAE010000413 DAEMAE010000000
VERSION     DAEMAE010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.421"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            151..>1039
                     /locus_tag="KPH93_003773"
     CDS             151..>1039
                     /locus_tag="KPH93_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG1690485.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIIAQKTNKNSKTISCIFQL
                     FDKDNCDEVDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSDNISYDVYIFALEIL"
BASE COUNT          421 a           94 c          169 g          355 t
ORIGIN      
        1 aagattttag aaataaatct taagctatct tatttaattc taatgcagga agaaggtaaa
       61 tgtatattga aagaatcata gaaatagaag taaacaaata caaaggtttg caaaagaagt
      121 aatataatta tatacaatga ggatgtgagt atgatgaaca gactaaagcc aagaatttta
      181 ggtattagta tcttgtttat tattataatt attttaacat gtggagctgt tttcaagttt
      241 aaatttaaat tagataataa gttaaaagaa accacacata catatttgtc tgagtttaca
      301 gaacacaata taattaatct caagacaagg cttagtggtc aatttgacat gcttgaatct
      361 attgctaact tcataggaaa cttgaataat ataaatgatg aaatggtgat tgatttaatg
      421 gattctgatg taaagcgaag ttctttaatt agaatgacta tctctacatt agatggagaa
      481 tgttactcta atgatggaat cactcataat gttcaagata gagattattt taaaaaagct
      541 aaaaatggag aaaagaatat atcagagcct ttaaaatcta ttgttaataa taaagaaata
      601 atagttttat ctaccccagt ttataaaagt gggaaaatag ttggtgtttt aagtggaatt
      661 tatgattcaa aaaagttaaa tgaattattg ggattatctg catttgatga aaaagcagat
      721 gtttttgttg caaagaaaaa tggagatata attgctcaaa aaacaaataa aaatagtaag
      781 acaatatcat gtatatttca gctttttgac aaagataatt gtgatgaagt tgattgtcta
      841 aaaactataa aagaaaatat gaaaaatgat aagtctggat attttaatta taacttagat
      901 aatgaaaaat ttatgttaaa ctatcagcca cttggagtta atgattggta tgttttttct
      961 gttgtgccaa ataaaatagt gtctattcag tcagataata tatcttatga tgtatatatt
     1021 tttgcattag aaatactac
//
LOCUS       DAEMAE010000414         1017 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEMAE010000414 DAEMAE010000000
VERSION     DAEMAE010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.422"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..531
                     /gene="vanZ1"
                     /locus_tag="KPH93_003774"
     CDS             22..531
                     /gene="vanZ1"
                     /locus_tag="KPH93_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG1690486.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVVIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(633..827)
                     /locus_tag="KPH93_003775"
     CDS             complement(633..827)
                     /locus_tag="KPH93_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG1690487.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNSNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
BASE COUNT          380 a          115 c          124 g          398 t
ORIGIN      
        1 aatatagtaa ggaggtaata aatgaaatct agaaaacata atattacaaa aggattattt
       61 atagtttaca taattatttt aacatggata atactattca agttgcaatt tgatatctca
      121 tcccttgaaa ccatgaatct tagaagcata aacttagtac cttttgctgg gtcacttata
      181 attaataata gagtagatat atcagagatt atattaaatg tggttatatt tgtaccattt
      241 gggatatatg tgtgtatgct aaaagaagaa tggagcttta taaaaaaagt tatacctatt
      301 tttataacta gtttggcatt tgaaacactt caatatatat ttgcactagg tgcaagtgat
      361 ataacggatt tgataggaaa cacactagga ggaatcatag gaatagctgt ttttatgcta
      421 ttatcgaaaa tatttaagaa taataccata aaaattatca atgtattggc attgattgtg
      481 acaattattg ttgtattatt tttaggatta gttatttttg caaacttata gaaataagta
      541 tatatgttta gcttaaaata aaacgtaaga tacaattaca tataaaatta tttacacata
      601 aaaagcacct atacaggtgc tttcctaaat tattaaagaa ttattgtatc ttgaaactct
      661 tgtatgttca attcttgatt ttctcttgtt ttttctaata aatctaacgc acaatttata
      721 caaactttaa tttctgcatt gctatttttc tcataataag ttatatattt atttctatat
      781 ttatctattt cttttccaca acgactacac attactatta cgctcatatc ttagttcctc
      841 catctctaat tattcttaat atattcaata gtatcacttt tctgaaaata atgattgtta
      901 aaactgtatc aaatttagat ttacttttta ttataatgca ttttaataaa aaaataaagg
      961 ttatgggtaa aatatttata gaaaaaagta tattattgaa tagattataa aatttta
//
LOCUS       DAEMAE010000415         1011 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEMAE010000415 DAEMAE010000000
VERSION     DAEMAE010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.423"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..>1011
                     /locus_tag="KPH93_003776"
     CDS             99..>1011
                     /locus_tag="KPH93_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG1690488.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQNKFEHYLKNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEHLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLTNIRGTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVARNASDLKLYTEDLIIDELLDKIFGDKKYNLNYDEKSYLKQLIKLRTKDKKATI
                     KNIDKNKLQDIMYEFFREIDKKYNSNFL"
BASE COUNT          464 a           81 c          160 g          306 t
ORIGIN      
        1 taaaattcta taaataacaa taaaaggttg attttataag aaaatatttt ttatactatt
       61 tgggaaaata caataataac actgagggag gtggggatat gagagaacgc attattcttg
      121 aaacattaaa aaatagtagt ggttatctaa acatagatta ttttgcagat aagttggggg
      181 tatctactcg aactataaga aatgaaataa aaaagataga aactataaaa gaatgtaatg
      241 gtttcaaatt agaatataag tctaaattag gatatatcct aaatattaaa gaccaaaata
      301 aattcgagca ttacctaaaa aacttaccct cagatttagt tgaaaatcca gaacaacggt
      361 tagagtctat aatagtagaa cttttagtaa atgaaggtta taaaactata gaacatttaa
      421 gtaaaaaatt tttagtaagc tcatctcaaa taaaaaatga tttaaaaaag atagatgaaa
      481 agataaagga cacagaatta aaattggaac gaaaggctca ttatggaatt aaaatagaag
      541 ggtctataaa agaaattcaa agtatattgg ttgatagtta ttttagaggg aatagaaata
      601 taacagagta cagaaataag tttatagata atttaaaatt aactaatatc agaggtacaa
      661 taaaaaatgt acttaatgag catgatttag aagcaaattt aacagagctt gaggaaatat
      721 tagcacaaat tattatattg tatataagag ttagtatgag agtagcaaga aatgctagtg
      781 atttaaaatt atatacagaa gatttaatta tagatgaatt attagataaa atttttggag
      841 ataaaaagta taaccttaat tatgatgaaa agtcttattt aaagcaactt ataaaattaa
      901 ggactaaaga taagaaagcc actataaaga atattgataa gaataaactt caagatatta
      961 tgtacgaatt ttttagagag atagataaaa aatataactc taatttttta g
//
LOCUS       DAEMAE010000416          956 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEMAE010000416 DAEMAE010000000
VERSION     DAEMAE010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.424"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..877)
                     /locus_tag="KPH93_003777"
     CDS             complement(<1..877)
                     /locus_tag="KPH93_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG1690489.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYAVSASNGIIRPDILYSEELVTTLTSAAEQKTEEFTGIEINKNITT
                     RELNLQSGVDPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKSAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREAN"
BASE COUNT          300 a          146 c          122 g          388 t
ORIGIN      
        1 gatttgcctc tcttaaaaca tggtctgcta aaagtggtaa tattatagat tttatcttac
       61 actgttctat tcctgatgct cctgcttctt taaaatcttt aaattcaaca gtttcattta
      121 gagtttcttc tgtaatgttc ttgatattag aatcagtcat ttcgtttgtt ttttcaatta
      181 attcattaaa ttttatagca aaatcatttg aagtatttat tagttcacct tctgaggggt
      241 ctagtaatcc tctcataaac agagcatgtt ccatcataat atggtcccag aataattcta
      301 tttctttagc actttttgac tcaatatcta ttttattttc aaggtcaact acataagaac
      361 gatataaatt tgcttcatgt attatatgtt caagaagtag agggtagttt gatgtaaata
      421 tagtacatga tagtacacca tctaagactc tttctttaaa attaataagc ccatcaagta
      481 atcttattgc atcagagtta agttgagcta cataattcac taagtcttga ccaacttgtg
      541 ggtctacacc actttgtaga tttaattctc ttgtagtgat gtttttgttt atttctatcc
      601 ctgtaaactc ttctgttttt tgttctgcag ctgatgtgag agtagttaca agctcttctg
      661 aatataatat atcaggtcta attataccat tactagcact aacagcgtat gataataaat
      721 cttcaaattg ctttttatag tggtcagctt ccatagcaag attataattt ttatttgtga
      781 atcctgcttc taaaaaaaga gcatgctctt tcataattct tgaaaaaaat aaatgtaatt
      841 ctagtgataa aataacatat ttttgattat ctatcataat ttcctccctt tagaaaatat
      901 catatttata atactttatt cgtaatattt aaatttatgt atattataaa taaatt
//
LOCUS       DAEMAE010000417          931 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEMAE010000417 DAEMAE010000000
VERSION     DAEMAE010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.425"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..669)
                     /locus_tag="KPH93_003778"
     CDS             complement(<1..669)
                     /locus_tag="KPH93_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690490.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVIGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFITSKLLPRHMLGNV
                     TSYGFVQETIYELKMLLPYNFEILFVEYIFLLIGFVSVVFTAILINKSIYKVSTLISF
                     LFLIL"
     gene            complement(678..>931)
                     /locus_tag="KPH93_003779"
     CDS             complement(678..>931)
                     /locus_tag="KPH93_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690491.1"
                     /translation="TYNILDKSLDEDKYVLDNKEKRGIRSIEIDSNKIVAYTSRLNSI
                     LSWDNKFILYIINKNSKEIVYEGELQFNSSNSTLEFEIK"
BASE COUNT          353 a          130 c           76 g          372 t
ORIGIN      
        1 caaaattaaa aataagaaac ttattaaagt gcttacttta taaatagatt tgtttataag
       61 aattgctgta aatactacag atacaaatcc tatcaataaa aatatatact ctacaaatag
      121 tatttcaaag ttatatggaa gtaacatttt taactcatat attgtttctt gtacaaaacc
      181 atagcttgtg acattaccaa gcatatgtct aggtaacaac ttacttgtaa taaataaaga
      241 tattgtaagt actactgtat agctatacac tagaaataat atatttaata atttagatat
      301 ataaatattg attctatttt taggtagcat tataagtgta tatatagact tactcctacc
      361 attaaagtct ctataccaaa taaaaaaagc atagcaaata catgcaatta taccaaacat
      421 aaaccacgat tctgtagaac ccattatatt tctaaagctt gagaaaccta taacatttac
      481 tgtttctata gtatttccga tttttgttaa ctgttcactt ttaactgcat tttctattat
      541 gtaattatag tcatttaaat tttttactat gtttaaaatt acaaaagagc aacaacaaat
      601 tattataaat atataattct ttatatttct ttttaattca aattcgaata atcttattaa
      661 atctttcatc aactcaccta ctttatttca aattctaacg tactatttga actgttaaat
      721 tgtaattctc cttcatatac aatttcctta gaatttttat ttattatata taatataaat
      781 ttattatccc aagataatat agaatttagc cttgatgtat atgcaacgat tttatttgaa
      841 tcaatttcaa tacttctaat gcctcttttt tctttattat ctaaaacata tttatcttca
      901 tctaaacttt tatctaatat attataggta a
//
LOCUS       DAEMAE010000418          924 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEMAE010000418 DAEMAE010000000
VERSION     DAEMAE010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.426"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(107..>924)
                     /locus_tag="KPH93_003780"
     CDS             complement(107..>924)
                     /locus_tag="KPH93_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690492.1"
                     /translation="SIINIFYFIGIFIVFGLIFSITENLNNKFIFSSFGKTGIIVTGA
                     IGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKDNLYQSIGNFF
                     IGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNINGFIHIILGDIS
                     NFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFIVIVIFVISSI
                     TYLFGFDNLNISSLIVKYNIFISFFMTIGFIFSIVTLIISYILSLVSPFKNFS"
BASE COUNT          380 a          119 c           78 g          347 t
ORIGIN      
        1 attttaaaat atattgagaa attttttatg ctctactaaa atctttttta tgtaatattt
       61 ttatatgttt ctaacaaaac caaatattag tacaatatgt atttatttaa ctaaagtttt
      121 taaatgggct gactaagctt aaaatatagg atattatgag agttactatt gaaaaaataa
      181 accctattgt cataaagaaa cttataaata tattatactt aactattaaa ctggatatat
      241 tcaagttatc aaatccaaat aagtatgtaa ttgaacttat tacaaaaata actataacta
      301 taaagctcag accatttaaa ctatttttta aatcagcact actaagtgac atatgtgtag
      361 atatagaata cattaaaaac ataaatataa aatacttcaa tgaatataca ctaggtgtta
      421 acaacattat tcttataaaa ttggaaatat cacctaaaat aatgtgtata aacccattta
      481 tatttaaatt attaatcata cttacatata actttaaatc tatattttga gtcactttca
      541 tataactatc tggcagtaat atcttaaaaa ttaaaataat cattattgtt ccacctataa
      601 taggtgcaat tcctataaag aaatttccta tactttgata aagattatct tttttatagc
      661 tatgacttac atatcctagt ataccatcat ttttactttc tattggtctg aaaaacttaa
      721 ctgaatttat tttatgcatg aaaattaaac acattatcaa atgactcaat tcatgaacaa
      781 atgttcctat agctccagtt acaataattc cagtttttcc aaaagatgaa aagataaatt
      841 tattatttag attttctgta atgctaaaaa tcaatccaaa tacaataaat attcctatga
      901 aataaaaaat atttattata ctta
//
LOCUS       DAEMAE010000419          907 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEMAE010000419 DAEMAE010000000
VERSION     DAEMAE010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.427"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            13..873
                     /locus_tag="KPH93_003781"
     CDS             13..873
                     /locus_tag="KPH93_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1690493.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          328 a          101 c          151 g          327 t
ORIGIN      
        1 atataaggag tgatgttatt gaaaaaagat agaataactt tttcggaaat tttaatatta
       61 attgtaggct gtatattaat ggcaatttct cttaacttat tttttaatcc acatgcaatc
      121 gctgctggtg gtataacagg acttggagtt gttttaaatt cattattcgg tgtagaactt
      181 tggattgtca atttactttt aaatgttcca ttattcatat ttgcttacaa aatactttca
      241 aaaaaagatt gttttaaaac ggttttagga ataatttttc ttaccatagc acttaagctt
      301 actgcaaata tggctacatt agatataaca aatgatatgt atcttgcaat tatatctggt
      361 tctattttaa tgggagttgg tcaaggactt atttttagaa taaatggctc tacaggtgga
      421 actgatttaa tggcactcct actaaataaa tattttccta catttagtat acctgtttta
      481 atgggtattg tagactgcgt tgtagttgtt ttatcaggta tagtgaatag acaggttgaa
      541 attgcacttt attcaactgt ggctttatac atcttagtaa aagttagtga cttgcttatt
      601 gaaggattta attattccaa atcattcact attatatccg acctttcaaa agatataagt
      661 aaaaaaataa tggaagattt agatagaggt gcaactattc taaaaggaga aggtgcatat
      721 actggagaaa ataaaaatgt acttttagta gtagttgaaa agaaagaagt tgtagaatta
      781 aaaaagttag taaaaaatgt cgaccctaat gcgtttatta taattacaga tatacatgaa
      841 gctttaggta atggatttaa aaaaatagaa taactaacaa ataaatagca agtataatta
      901 acatata
//
LOCUS       DAEMAE010000420          903 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEMAE010000420 DAEMAE010000000
VERSION     DAEMAE010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.428"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(140..646)
                     /locus_tag="KPH93_003782"
     CDS             complement(140..646)
                     /locus_tag="KPH93_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG1690494.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTVSGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKILVKE"
BASE COUNT          313 a          140 c           89 g          361 t
ORIGIN      
        1 taaatatagt tcatcttttt tataatagta tttatttaaa gttaatattt atttagttag
       61 tagcatctac ttagatatca gataagaaag cacagtcatt tacttataat aagttctttt
      121 cagatatttt ttattataac tattctttta caagaatttt tataatttct gaaatgtaca
      181 tagtatgata gtctttctct ggataccatt ttttatcatt ttctttagca tcaaagtttt
      241 ctggtggcat attaccatga tataactttt tacatactat aatcatatta gcttcttcaa
      301 aagctaccgt atcatccaca aaatatggtg taagtcctgc tttttctatt ttatttatat
      361 ctctaccgga aacagtacca caaatattta aagcttctct atacgtttca tcaaagaagg
      421 caactgtaaa tgtatcatta ctatctacaa attcttttgt atatctctgt ggtcttatgt
      481 aagttgtaac aacattttta ccccaatata ctccaacacc accccagcta gcagtcattg
      541 tgttaaactt ttccgaatca ccagctgtta taagtaacca ttctttacca atcttagtaa
      601 atggattaaa ctgtagttcc tcaattttta cttctctaaa acccatgact tcatcctacc
      661 cttcattttt taattatata aattaattgt taaaaatttg aatatatgta ctcttataat
      721 actgactttt gattgtccta cataattcat gtaaattata atacaacaaa tcaattactt
      781 tcgcaataag tagtttttat attttataag tgtaaaatat agataaaata tattgattat
      841 aaagcatata atatattttt aaacctaaag taatatacga taaaaaaatg tagaaagtaa
      901 gta
//
LOCUS       DAEMAE010000421          900 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEMAE010000421 DAEMAE010000000
VERSION     DAEMAE010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.429"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            434..>900
                     /locus_tag="KPH93_003783"
     CDS             434..>900
                     /locus_tag="KPH93_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG1690495.1"
                     /translation="MNSNTFERLQLNEVKELIQIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPIELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKVRR"
BASE COUNT          405 a           73 c          138 g          284 t
ORIGIN      
        1 tgataattta tagagctaaa ttctttgata tataatgatt acaaagttag actctattgg
       61 aaactgcaca tacatgaatt gatgacaata taagtgattc caaaatataa atgaaagtat
      121 aagcttattt tcactataaa aattttcact atacaaaaac attaatgaca ttatttttat
      181 tgacataaat atataattta tggaataata ttagaagcac tagtataagt gtttaaataa
      241 gaatagaaaa ggagaagtgt tttatgaaaa aaaatactaa cattaatgag tccggagtta
      301 aacttcttaa taaataagga gtttgatata taataactcc ttatttatta agttgttcgc
      361 aaaataacta ttatcaaagt aaatgatata gtgatttaaa agatgatgac ttataaataa
      421 aggagaaaaa ataatgaata gtaatacatt tgaaagatta caactaaatg aagttaaaga
      481 attaatacaa atacattgtg taagttcgct aggaaaaaat ttaatagata aattaactcc
      541 tagtgggaat attggagtag taagaaggaa attagcagaa aataaagaag ctagaaagat
      601 aattgaaaat agtaatcaca taccacttga aggattgttt aatgcaggtt ctacaataga
      661 taaaattgaa aaaggaatga taatagagcc tattgaactt gtaaatattg aagatttttt
      721 aagaggttgt aggaagatga aagcttttat gttagaaaaa gaattttatt cacctacatt
      781 aagttcttat gctttaaata taacagaatg taaaagtata gaagatgaga taaattactg
      841 tataaaatct aataaagttg attctaatgc tagtaaagaa ttaaaaaaag taagacgaaa
//
LOCUS       DAEMAE010000422          899 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEMAE010000422 DAEMAE010000000
VERSION     DAEMAE010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.430"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>899)
                     /locus_tag="KPH93_003784"
     CDS             complement(<1..>899)
                     /locus_tag="KPH93_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG1690496.1"
                     /translation="NILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMSTFIYL
                     TISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIVLINVCISMFNLLYLLNK
                     LDSNIERYKLIKDLTYFVFFTTIICFVAISKINIVDSDRVIGVIYLLLCNHTYYVYVY
                     SLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKELLRQA
                     LDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKKFDND
                     ISIIDKLVELNDKIYLLSLSNNK"
BASE COUNT          330 a          124 c           68 g          377 t
ORIGIN      
        1 ctttattatt acttaaactt aacaaataaa ttttatcatt taactctact agtttatcaa
       61 ttatacttat atcgttatca aatttcttca atttttcagc aactttttca tcttccttta
      121 taaaagttaa tattttataa taattattca tatcctttga aaattcatct ttaaatttat
      181 tatttgtgta acttatatta aaatcatagt ctgttactat ccatacattt ggtattgtat
      241 ccagagcttg tctaagtaat tcttttctct ggtctatgta gttttttaac gttctttgta
      301 taatcatatc cttttctatt gctaaattta tctttcctaa ttgctctgac tttttactta
      361 attttttatt tattgaatca agttcataat atggatacac tactctatta ttaagactgt
      421 atacatatac ataataagta tggttacata atagtaaata tattactcct attactctat
      481 ctgaatctac tatattaatc ttagaaattg ctacaaaaca tataatagtt gtaaaaaaaa
      541 caaaatatgt taaatctttt attaacttat agcgctcaat gttactatca agtttattca
      601 acaaatatag taaattaaac atagatatac aaacattaat taatacaata atcatattcc
      661 agttaatatc actaaaaata tttcttataa agtaatctaa tatcacaaaa gcactataaa
      721 aaattagtat tgggcttatt cttagttttt ttactttaga aatggttaag tatataaaag
      781 tagacatatt aattatgctt aagttaagca acacaatttg gaatttttga atttttaaat
      841 ttactactcc taaaatactt acaaaaacta atacaagaca ccctactttc aatatattt
//
LOCUS       DAEMAE010000423          897 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEMAE010000423 DAEMAE010000000
VERSION     DAEMAE010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.431"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            224..>897
                     /locus_tag="KPH93_003785"
     CDS             224..>897
                     /locus_tag="KPH93_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1690497.1"
                     /translation="MNNSFLDKLIETRELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSHVFDTIIDSLKEKITHNFLRNLHSSLIFNTTLH
                     FRGMSGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDF"
BASE COUNT          353 a          104 c          135 g          305 t
ORIGIN      
        1 cacaatgaaa tgttgtacaa agtgatacat aataattata tgaactataa aattgactaa
       61 acttattgac taataaatta gatgaaaagt tgagacttac tgaaaataaa tacaatgaaa
      121 tatatactac aagtattaaa ttaaatctga actttacata ctattagaat aagtattata
      181 agattataat gatttaaaaa tattaaaata attgaggtga tacatgaaca attccttttt
      241 agataaattg attgagacaa gagaacttaa aaattcactt tataatgtgt taaaacacaa
      301 ttttctttat cattctaata agatagaggg cagtactttt actactgaag cattagcact
      361 tttacttgat aaaaatgttg ttacaggaag acatacttta gatgatgtac aagaaactgt
      421 taattctagc catgtgtttg atacaataat tgactcactg aaagagaaaa taacacataa
      481 tttcttaagg aatctacatt caagtttaat cttcaataca accctacatt tcagggggat
      541 gtctggtata tataaaacta taccaaatat gatactagga actgatgtaa gcatagcaca
      601 accatttgaa gttgagccaa agcttgatga attaattgaa tggtactata gtcagagtga
      661 agtatctatt aaagttatag ctgaatttca ttatagattt gaattgatac atccatttca
      721 agatggtaat ggtcgcatag gtagatttgt tatgctaaaa cagatgttag aaaataatct
      781 tcctataaaa attgtatctt gggatagtga ggacttgtat agaaactcct taaattcttg
      841 tagtttaggt aattatgtcc ctttaattga atatttatca agtttagaag attttag
//
LOCUS       DAEMAE010000424          894 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEMAE010000424 DAEMAE010000000
VERSION     DAEMAE010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.432"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            24..>894
                     /locus_tag="KPH93_003786"
     CDS             24..>894
                     /locus_tag="KPH93_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG1690498.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDAFVSYKLTDNESTLK
                     KYPYSFELVINYTLNDNSIGVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSRNDVEKINLNGSFTDDIISIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYNETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
BASE COUNT          349 a          107 c          133 g          305 t
ORIGIN      
        1 aaatataata aaggagattt taaatgaata tattacaaaa tgaaaattta attatcgaat
       61 caagcagttt tggtgctgaa ctaactaaaa tattttctaa aaaatacgac aaaaacattc
      121 tttgggatgg taataaaaaa tattggggta gacaatcgcc tatattgttc cctatagtag
      181 gtaaactttt tgataatgaa accataatag aagaaaattt atatagcatg actcagcatg
      241 gatttgctag agatatggat tttgagattg tagataaagg agatgcattt gtatcttaca
      301 aattaacaga caatgaatct actttaaaaa agtatccata ttcttttgaa ctagtcataa
      361 actatacatt aaacgataac agtattgggg tagaatggat tgtgaaaaat actgattcta
      421 aagatatatt cttttctata ggtggacatc ctgcattcaa ccttcctttt tatgagcaaa
      481 atgatttttc taaacactac cttgaattta aaagtagaaa tgatgtagaa aaaatcaatt
      541 taaatggttc ttttacagat gatataatat ctattggaaa attaaaaaac ttacagttaa
      601 atccagaagt attcaagaat gatgctttga tatacaccaa catagatgaa gtatctatat
      661 gtaataatga tggcagtaaa tatgtaacta tatctatgga agatttccct ctagtcggta
      721 tttggactcc ttattataat gaaactaatt ctactgctcc ttttttatgt atagaacctt
      781 ggtatggtct tgctgatagt ataaattcta acaaaattta taaagataag aagtttatta
      841 ataaactgag taaaggaaaa gtttttactg cttcttacaa tataaatata cact
//
LOCUS       DAEMAE010000425          889 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEMAE010000425 DAEMAE010000000
VERSION     DAEMAE010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.433"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            77..466
                     /locus_tag="KPH93_003787"
     CDS             77..466
                     /locus_tag="KPH93_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1690499.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            459..>889
                     /locus_tag="KPH93_003788"
     CDS             459..>889
                     /locus_tag="KPH93_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690500.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFIFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKF"
BASE COUNT          349 a           76 c          125 g          339 t
ORIGIN      
        1 tgtttgttat tattttttat gttatactat gtatataaca atcgttttca tttataaagc
       61 ataatggagg ttttttatgg ttttaaattt agattttaat agtgataaat ctatttatgt
      121 acaaattaaa gaagaaataa ctaaggctat agcttcagga gaattgaaaa taaatgaatc
      181 acttccatct gtaagaagta tggctgaaaa tattggtgta aatttacata cagtgaacaa
      241 atcctacaat gaattaaaag aagagggatt cttaaatatt gatagaagaa aaggtgctat
      301 tgttgctcaa ctaccaatga aaatagataa tacaactcgt caaaaaaata aacttgactt
      361 ggaattattg gtggcaaaat cttatttatc aggtattagt aaagaagaat ttttgtcatt
      421 aagttcaagt ttatttgata agtatgaggt gaaatattat gagtaagatt gaatgtctat
      481 ttacaatatt tccagttcta attttgatgt atgctacatt ttattttatg ccctattttg
      541 taggtaaaaa acacatctat ggagtttcta tagaccaaga gcataaaaat tataattact
      601 ttataaagtt agataaagaa tttaaaaaac tgttatcttt aggttttatt atagacttta
      661 tattagtttt tattctggtt tttatattta ataaattgga actttcctat tttattggta
      721 ttataggttt tttattatat gaaagtatac tatatattca tacacataaa aaagctaaga
      781 agcttaaatc agaaatttat tctaaattag gtcatataga tatagattca aaattaatag
      841 tagatatgga ttttataaat aagaaaaata aaataataaa aaaatttaa
//
LOCUS       DAEMAE010000426          855 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.434, whole genome shotgun sequence.
ACCESSION   DAEMAE010000426 DAEMAE010000000
VERSION     DAEMAE010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.434"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            34..813
                     /locus_tag="KPH93_003789"
     CDS             34..813
                     /locus_tag="KPH93_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690501.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIIEHLLDTSKQNEDKKIYNYL
                     LSLNKGYSLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLKDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT          360 a           83 c          121 g          291 t
ORIGIN      
        1 ttatacacaa gatatttaaa aggaagtgat gatatgaaag ttaatgaaaa taatataaat
       61 aatatagatg aagagttaga tgaacttagt aggaaagttt acttagagtc accagatttt
      121 tggataagta tttctgacaa aataatagat aaagtatttg atgaaatgtt attatttttt
      181 gcagttaaat ataactattt caacatagta tgctttgcta ttgaaaacaa ttatctggat
      241 ttaaacatgc cttcaaaaaa caaaaaatac actaatataa tagagcattt gttagataca
      301 tcaaaacaga atgaagataa aaaaatttat aattatcttc taagcttaaa taaaggttat
      361 agcctagatg aaagaccaat tttagatgaa ttaaactgta atattagtag tgatactttt
      421 gaaaaaaaag atatatatct tccaaagtac ttatgtccaa gttgtaattc taatatattt
      481 gatacaggtt ataaagtatc aaatgacata acatataaat tttcttctaa agaagctgaa
      541 cctattgaaa tgtgtagtga agtttcttct atcagatgtt gtaattgtga tgaattatta
      601 aaagatatta cacttgataa actatataac ctatgcaaaa ttcaaaactg taataattgt
      661 ggttctaatt taactaaggt aggaattatt gcaaagaaaa aaatgaactt tgatgagaat
      721 actgaaaaat ttaaagatgt ctcaacatct tactgttgtg gaaattgtga ctctgaaata
      781 aacttatctc aaagagaaca ttttaagatt taaatattta taggctaaag taataagatt
      841 acattattag atact
//
LOCUS       DAEMAE010000427          805 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEMAE010000427 DAEMAE010000000
VERSION     DAEMAE010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.435"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            273..416
                     /locus_tag="KPH93_003790"
                     /pseudo
     CDS             273..416
                     /locus_tag="KPH93_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
BASE COUNT          338 a          101 c          129 g          237 t
ORIGIN      
        1 ttatataaaa taaaatctta tataaaaata tatcatatta taatttacaa gtatagtcta
       61 aatagagctg tttttaagca aagttttata aatgactatt aaagtataaa gtaacagtat
      121 aaagtttact tataaattaa atgtaaacct aaatacttat aatagttgac aaaaaaacgt
      181 ctttatttta tactaaaaca aagctttaat agaagctttg tatatgtaca tataaactaa
      241 atgaaataag acatatgaag ggaattataa aaatgaaaaa tactaaagta aatattcaag
      301 atttaactaa aatgtcaata tgtgtagcat tattgtgtat atcatcttac atatatatac
      361 cattgccatt tactccagca ggagtaacag cacaaacaat aatgataaat ttgattagaa
      421 aatagctacg cacacttgtg actttagtca tgagttagta gctaaaatag tcagcatata
      481 gggaaaccta tatgtagaga taggatagaa cctatccaat actacttgaa ttgctgggaa
      541 cccctaaagc taatcaaact aaaacataag gatgaaataa acctaagtgt gaaagttacg
      601 aaagtagaaa aaattggtta gatggtataa ggttaaaccc taagtacttt aaaatgggca
      661 atcagcaggt aaactccgaa taggagaaac ttcaacgact attcctcttg agggaagtac
      721 actacaagct attggtagtg gaagtgggta gaccttaacg gataatgccg aaggataaga
      781 tatagtctgt gcttacatga aagtg
//
LOCUS       DAEMAE010000428          796 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEMAE010000428 DAEMAE010000000
VERSION     DAEMAE010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.436"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            44..685
                     /locus_tag="KPH93_003791"
     CDS             44..685
                     /locus_tag="KPH93_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG1690502.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQELLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGVPDGNLPNAREVVYKVSRV"
BASE COUNT          307 a           91 c          156 g          242 t
ORIGIN      
        1 atttagtatt tgtactaaga aatacataaa aaggggaata gatatgaata ataattttca
       61 agacaaccaa acaataaatt taatacaaag tcgtagaagt atacgaaaat ttacaacaga
      121 acaaattagt gatgagcaag taaacacatt actacattgt gcatttgcag caccttcagg
      181 atgtaataaa cagccttggc acataactgt tgtgcaagac caggaacttt taaaagagat
      241 tagtgatgat actttatcta ggattcatga agtgagtaat gttgagataa ataaaaactt
      301 taagttattt tatggtgctc caaccgtttt atttatttct tatgatgaaa gtagttcatg
      361 ggctccatat gatataggaa tacttacagg gaatataaca acagctgcac aagcactagg
      421 gcttggaagc tgtataatag gaatggttag aggtttattt acacctgttg aacaagggga
      481 tattgaaggt cttgtgagtg tattggataa agaagatgta aaagagtcag agtcaataaa
      541 gatgaagttt gatacaaata aaaaatacag agaactactt gatataccag aagggtattc
      601 tgtaccattt ggtattgctg taggtgttcc agatggaaat ttaccaaatg ctagagaggt
      661 ggtttataaa gtttctagag tttaggataa agtattgcaa caactaatca gttacttgaa
      721 gaattaaatc agtaatatac aaataaatta atgtaagagg aagctattta aaaagtaatt
      781 tagatttttc attgta
//
LOCUS       DAEMAE010000429          783 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.437, whole genome shotgun sequence.
ACCESSION   DAEMAE010000429 DAEMAE010000000
VERSION     DAEMAE010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.437"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(102..290)
                     /locus_tag="KPH93_003792"
     CDS             complement(102..290)
                     /locus_tag="KPH93_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690503.1"
                     /translation="MNKNNNLVIICMFIGMILGMSIGCAIGISKGNVGITMCYGLVFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT          292 a          134 c           53 g          304 t
ORIGIN      
        1 tagtatctta tttagtatgt cttattcgat tttttacaga taattaaaaa gttttatcac
       61 aaataaatca taattagaca tcctagtaaa tcaaatttca tttattcttt tttattagag
      121 tttttgataa cagtccctat acatatgcca attatcattc caaaaactag accataacac
      181 attgtaatac ctacatttcc tttagaaata cctatagcac aacctataga cattccaagt
      241 atcatgccta taaacataca tataataact aaattgttgt ttttattcat agtttttctc
      301 cattccatat attttaaatt gcctagctcg ccttcttagc ttaaaacaaa gaaatataaa
      361 ttaactttat tttattctaa tatctgcttg tataaatgtc aaacaacaca tttcttgtca
      421 taacttcctc tttttgaagc aaatataatc tcccagacac aataatatca attttccaaa
      481 actatttaaa taacttttat atttacataa tatttacaat cataaactat attgttattc
      541 atttatacat cataaacact ccctcctaat cattattata cttaaagtaa tgtccattta
      601 taataatata tctaacgcac tttaattgaa aaaactaaaa ctttgccttt cattcccata
      661 tataaataag tatacttttt gatgctctat tacaaatcaa gtaaattaat tgatataaat
      721 tatcattttc tgttgaaaat accattcgca catgctatac ttaattgcat aaatggtaat
      781 aaa
//
LOCUS       DAEMAE010000430          772 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEMAE010000430 DAEMAE010000000
VERSION     DAEMAE010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.438"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            215..397
                     /locus_tag="KPH93_003793"
     CDS             215..397
                     /locus_tag="KPH93_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690504.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDIVE
                     LCLHRVMILLCECGLA"
BASE COUNT          278 a           93 c           84 g          317 t
ORIGIN      
        1 ctactaactt aaatatttat aaccaaactt taaactggat aaattttcct ttaagaatga
       61 aaatttatcc agttttgcga ttataataac cagttttaca tatccacttg taaaatatat
      121 ctaagcaata tatcatacta tatagattta tgagtgaaat aatatctgct atttactgaa
      181 tatttttatt tttcgttttt tagggggatt agttgtgttt aaagataaga ttgatgaatg
      241 tgttcatata atgactgcat atattgctag tttaaaagaa tactattcat tcatagaaac
      301 tcaaattggt gattttatta aaaaatatgg agaagatatt gtagagttat gtctccatag
      361 agtgatgatt ctactatgcg aatgtggttt ggcataaaat taaatgtatt aaaatgggta
      421 agaagaatcc tatataatat tcttcttacc cattttaata tatcaatttc atagtttatc
      481 gtttaaaata ttttacatcc attactagac aactttacat tccattttta attctaattt
      541 atcacttaat tttttatact ttttaaatcc tatacctaaa acattaagtg caactattag
      601 aattaataat tgttgtggta taaaattatg cttgcttcat atttattcat tttttcctat
      661 aaaagttctt gttttacaaa aaacatttgt tttgtatcaa aataatactt ataaattaca
      721 taattctcat aaaaatttct agtatcactt gtactcttaa taaataatag cc
//
LOCUS       DAEMAE010000431          770 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEMAE010000431 DAEMAE010000000
VERSION     DAEMAE010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.439"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(124..513)
                     /locus_tag="KPH93_003794"
     CDS             complement(124..513)
                     /locus_tag="KPH93_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690505.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETRSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
BASE COUNT          269 a          119 c           73 g          309 t
ORIGIN      
        1 actatcagat actaaggaaa taagcacttt tctttatata atgttctacc atcacataac
       61 actaacatta aatcaaaacg ctactctcaa ttgtctcaaa ctaaaaagat actgatagat
      121 ttattagtca tcaatatctt cttctttcaa atttttaaag tattcttcta atttgtccaa
      181 gctcttacta tctaaaactt catcatcatg tagtgctgaa ataaaactct taaatgaatt
      241 gttatgcata aaactaaaaa aactccttgt ctcaactttt aagtaatctt tctcttttat
      301 agctatagta taatgcgtac atctatctac cttatcagta gttaaaaagc ccttatctac
      361 taaatttttt aaaaatgtca atgttgtgct ctttttccaa tcgtatttca acttcatgtc
      421 ttctatgata tctcttgagg ctaatacatc atctactccc caaatatact tcattagttt
      481 tagttctgca tgtggtaatt tattaatctt cattagttta caactcttac caatttatat
      541 ataactaaac tttataacaa taagtgtatg taatcaatta gattgggata aatataaatt
      601 ccaatttatt ctatataaaa ataattaata aaaaagaagt actctacttt agagagtact
      661 ttgggattat atattcaaaa atttatctaa catgattaga gtatgtttta tgctttagtg
      721 tgataatttt agttcgtttt attattataa tatgtattat atttcattat
//
LOCUS       DAEMAE010000432          761 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.440, whole genome shotgun sequence.
ACCESSION   DAEMAE010000432 DAEMAE010000000
VERSION     DAEMAE010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.440"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            96..296
                     /locus_tag="KPH93_003795"
     CDS             96..296
                     /locus_tag="KPH93_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690506.1"
                     /translation="MKKLKIISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEGSKRYKF"
     gene            complement(492..>761)
                     /locus_tag="KPH93_003796"
     CDS             complement(492..>761)
                     /locus_tag="KPH93_003796"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1690507.1"
                     /translation="GSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSE
                     LIYLIDTTDPKEMISLINDYIYFYNNERIQLKNGMSPIEYRTHSE"
BASE COUNT          278 a           86 c          103 g          294 t
ORIGIN      
        1 aaaaaaatat atattcatga tttttatata ctatgaaata aaatatattt aatattatat
       61 agataaattt gaagtttata agaaggagga taagcatgaa aaaattaaaa ataattagca
      121 ttttttcttt gattataagt gtaattttaa cgattggagg tattggaata gtaacttatt
      181 atgtagataa tttgtttatt agaggactat cggtatttgt tttaattatg tcctcttctt
      241 ttgtatcaac aacagttaga ttgatatttg aagggtcaaa acgatataaa ttctaattta
      301 aaaattggat taagtaaata attttaatat accaaaaatt acagagatta gctcaaaata
      361 gactttttag tccatgaggc atttttttgt atgaaattaa atatattttc atcatttcaa
      421 caatgaaaaa gaggcttatc tctattttgg atatgtccct gtcaagtaga cagacttaaa
      481 aaaggctatc tttactcgga atgagttcga tattcaatcg gactcattcc gttttttaat
      541 tgtattcttt cgttattata aaaataaata taatcattta ttagactaat catttccttt
      601 ggatctgtag tatcaattaa atatattaat tcagatttaa aatgactgaa aaaactttct
      661 ataggagcat tatcccaaca gtttcccctt cgggacattg attgtataat accattatcc
      721 ttaagccttt tagagtaaga tctacttgta tattgtgaac c
//
LOCUS       DAEMAE010000433          692 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.441, whole genome shotgun sequence.
ACCESSION   DAEMAE010000433 DAEMAE010000000
VERSION     DAEMAE010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.441"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..>692
                     /locus_tag="KPH93_003797"
     CDS             88..>692
                     /locus_tag="KPH93_003797"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1690508.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLIGVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAK"
BASE COUNT          302 a           60 c          113 g          217 t
ORIGIN      
        1 cagatttaaa aatattagtc tagaaaatga taatagaaaa tttaaatctg ttgaagaaga
       61 attgaagtac ttacgcatgg agaatgaatt cttaaaaaag ttaagtaccc tattggacaa
      121 gtcaaagtaa gcgataaatt taaaacaatt aaacttctgt ctaggaagta tagtatttct
      181 agtatgtgta agttgatagg tgtgtcaaga agcggttact ataaatggct aagttattct
      241 aaaaaatctt ccgatagggg tatcaaagat agaattatta aggattatat aattgaaata
      301 cataaaaaat atagaggaac ttatggtaga aaaagaattt gtacttattt aaataaaata
      361 cttgataccc ctattaatca taagagagta tataggttga tgaaagaact aggtattaaa
      421 tctattatta gaaagaaagt atatagacgt aagtttaaat catatgagat atatgataat
      481 atattaaatc gtgaatttag agctaatcaa ccattagaaa aaatttgtat ggatataaca
      541 tacataccta tcggtaaaaa attcttatat atgaatgtag ccaaagattt atttaatggt
      601 gaaatagtgg catatgagat aagtacaaaa atggatacta aattagttaa taatacagta
      661 aatcaactaa taaatatgaa tttagctaaa ga
//
LOCUS       DAEMAE010000434          673 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.442, whole genome shotgun sequence.
ACCESSION   DAEMAE010000434 DAEMAE010000000
VERSION     DAEMAE010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.442"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>673)
                     /locus_tag="KPH93_003798"
     rRNA            complement(<1..>673)
                     /locus_tag="KPH93_003798"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          138 a          192 c          142 g          201 t
ORIGIN      
        1 cagtctgact cccaagtata agatgacggc attcggagtt tgatagtctt cggtaggtgc
       61 aataccccct aggacattca gtgctctacc tccgtatctc tcaccttgag gctagcccta
      121 aagctatttc ggggagaacc agctatctcc gggctcgatt ggaatttcac cgctacccac
      181 aagtcatccc cgagcttttc aacgctcgtg ggttcggacc tccacgaaat tttactttcg
      241 cttcatcctg ctcatgggta ggtcgcccgg tttcgggtct acgtcaagta actgaacgct
      301 cagttaaaac tcgctttcgc tacggctccg caccttaagt gcttaacctt gctacttaac
      361 gtaactcgtt ggcccgttct acaaaaagta cgcggtcaca caagaaatgt gcttccacag
      421 cttgtaagtg cagggtttca ggttctattt cactcccctc ccggggttct tttcaccttt
      481 ccctcacggt actatacgct atcggtcact aggtagtatt tagccttgga gggtggtccc
      541 tcctgcttcc cacagggttt cacgtgtccc gtggtactct ggatcatatc tgaagtcttc
      601 tcgtttaaca tacaggacta ttaccttctg tggtgggtct ttccaaacct cttcaattac
      661 gatacctctt cgt
//
LOCUS       DAEMAE010000435          666 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEMAE010000435 DAEMAE010000000
VERSION     DAEMAE010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.443"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..>666
                     /locus_tag="KPH93_003799"
     CDS             67..>666
                     /locus_tag="KPH93_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690509.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDKLIPAKITSDQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNADN"
BASE COUNT          279 a           61 c          101 g          225 t
ORIGIN      
        1 tatttaaata taatataagt tttataaagt atattaaata tattataaga tggaggtcga
       61 gtaattttga aaggaaacat agtacaatat aattttgcag atatagagga agaagtatat
      121 tcgttggatt atgctatagc atggaataca gacgaggaaa acgtaaacat aataccattt
      181 acaaataaat tttgtaaaga atctatagag tctttttgcc taggtaaaat taataacttt
      241 gttgaaatat taaatgaagg ttttgttgaa aatcatcatt atgtacattt agataaaatg
      301 attagtgttc ctaagaaaaa agttaattta gtatatcaac aagacacgca tggttatctt
      361 ctaagagatg ataatgacaa gcttattcct gcaaaaataa cttcagatca atctaaatct
      421 atatcaagta agatggagtt attctgtgct ggtgaagaaa aatgtttaat taatatatta
      481 ctaaaggctg acccatcata tattttagat gtagatagta taaaagataa aaacattcta
      541 aatctaggtt atgagtctat tgatagatat aaagaataca attttgatga tgataaaatt
      601 ttaatatttt ttataaataa aaaacgttat tcagtgatta tgaaaaaaac aaacaacgct
      661 gataat
//
LOCUS       DAEMAE010000436          664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.444, whole genome shotgun sequence.
ACCESSION   DAEMAE010000436 DAEMAE010000000
VERSION     DAEMAE010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.444"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>664)
                     /locus_tag="KPH93_003800"
     rRNA            complement(<1..>664)
                     /locus_tag="KPH93_003800"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAEMAE010000437          649 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.445, whole genome shotgun sequence.
ACCESSION   DAEMAE010000437 DAEMAE010000000
VERSION     DAEMAE010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.445"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(259..639)
                     /locus_tag="KPH93_003801"
     CDS             complement(259..639)
                     /locus_tag="KPH93_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1690510.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRLLYVQETDKCMYYTVAIKERAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT          246 a           91 c           58 g          254 t
ORIGIN      
        1 tataaacatc tctaataatt aaaaacttta tataaagaat agaaacaagt tttagattac
       61 tatagagatg gcatatcaaa atatttttaa aaagtaatct aagtattaga agtaattgaa
      121 gttagtaaat ataaatgctt ttacttttaa gtatttctta agggaataaa aaataaaaat
      181 aagctctgca accatctaaa aatagttgca gagcttataa aaccaaaatt atctaaattg
      241 taataaaaat aataacattt atatataagc ttcttcccaa tttaccaccc agttttctaa
      301 cgactttatc ttttcctctg ttagctcctc ttcgtgaaat gatactaata aattccttat
      361 aaaattattc ttcaaaagat tttgcacatt tcttgatata aactctaaat atgccctttc
      421 tttgattgca actgtataat acatacattt gtctgtttct tgaacatata ataatctttt
      481 ctttgataat ttggataaag tttttaaagc tactttctct ggtaaattat gctcttcttt
      541 catataatca ataatctcat atgaattggt ttttatatct aaattccaaa taaatttcat
      601 aataactaac tctaattttg ataatttttt caaatacata gttatcact
//
LOCUS       DAEMAE010000438          639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.446, whole genome shotgun sequence.
ACCESSION   DAEMAE010000438 DAEMAE010000000
VERSION     DAEMAE010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.446"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            198..404
                     /locus_tag="KPH93_003802"
     CDS             198..404
                     /locus_tag="KPH93_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690511.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
BASE COUNT          280 a           45 c           70 g          244 t
ORIGIN      
        1 gatagatttc ttttaaaaaa tattttaact ataattgatt tttatttaat ctgaaaaaaa
       61 tattatgatt ataaaaaatt gctacttatt atttgaatat ttttgtatat aaataataag
      121 tagcatgact ttatacatta ataatgatag aatataaatt aatagataag atataatttt
      181 aaaaggagtg atataatatg agtaaatata gttctttatg ggaatatgtt aagaaaaata
      241 atagtcaatc gtttaaatta acttttgaag aaataaaaga tattgctgga atagagatag
      301 accattcgtt tttgaaatat aaaaaagaat taaatgaata tggatatcaa gtaggaaaaa
      361 tctctttaaa agagaagact gttattttca ataaaattga ttaagatata aataattaca
      421 tataataaaa taatttgtat tgacgattat tacaaataaa aataaagtaa atcttatata
      481 agatttactt tatttttaga tgatattttt tagctttata ctataaggta tataaaacta
      541 agcctattaa aaatgttata atttctatta ccatcataga ccatatcaat ccatcaagtt
      601 cataaaagta attaagcttt attaaatagc ttctttatt
//
LOCUS       DAEMAE010000439          610 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.447, whole genome shotgun sequence.
ACCESSION   DAEMAE010000439 DAEMAE010000000
VERSION     DAEMAE010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.447"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            77..>610
                     /locus_tag="KPH93_003803"
     CDS             77..>610
                     /locus_tag="KPH93_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG1690512.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGLALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDD"
BASE COUNT          277 a           40 c          107 g          186 t
ORIGIN      
        1 tatatggaga atttaattta aataaagaag aaataagaaa attaagaaat caatatctag
       61 agtaaaaggt gagtttatga gtttaaatat aacagcaaga cagataaata taataaaaat
      121 attgttaaat tcaaaagata caattagtgg tcttgcctta agtcaagaga taggatgttc
      181 ttcaaaaact atacaaaatg aaataaagga tattaataaa caacttagaa atggtaaaat
      241 tttgtcaata agaggagttg gttataaact agaaggaagt tttgatgagg tgaatctaaa
      301 tagcaatctc tatgatgatg tagatagagt agaatatata atcagaaaaa tattaacctt
      361 aagtaatgaa gaaaaaaata ctataaaact tgaagattta gccgattcta tgtatgtaag
      421 tgtttcgacg gtaaaaaatg atttaaaaga agtaaaaaga atgttggaaa agtattcaat
      481 atcagttaaa tctaagcata aacaaggtat atgtgttttg gaaagtgaag ataagatatt
      541 aaattgtata gttgacttaa gtaataagag agataatcaa ttgagtttaa atgatttttt
      601 aaatgatgat
//
LOCUS       DAEMAE010000440          604 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.448, whole genome shotgun sequence.
ACCESSION   DAEMAE010000440 DAEMAE010000000
VERSION     DAEMAE010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.448"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(98..460)
                     /locus_tag="KPH93_003804"
     CDS             complement(98..460)
                     /locus_tag="KPH93_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG1690513.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMIQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT          211 a           94 c           37 g          262 t
ORIGIN      
        1 atttgaattt gctaattaat atttataaaa attagattta atacataaat ttataggtgt
       61 attaattaat aattttaatc aacacaccta tctctttcta atcttctttt agcaaagtac
      121 ttttccatac atcttcttta aagcctaata atacattttt accatccaaa agtaatggtc
      181 ttttaataag cataccatca cttaccaata tttctaaaag tttatcatca gattcagttt
      241 ttacaatatc tttaagaccc aattctctat atttcatacc acttgtgtta aaaaatttct
      301 ttatatcata tccactagtc ttataaattg attttaactc ctctttacta ggagggtttt
      361 gaatcatatc tatatcttca aattctaaat tattttcctt aagccaattt ttagcttttt
      421 taacagtaga gcattttgta tatccatata atttaatcat tttataaaaa ttcctttcta
      481 attttttatt gttataccca taatttgtat actataacca atttatatca taatcattat
      541 gagctttcaa cataaaaaat tatcatattt caaattatta aacatttatt cacattatat
      601 attt
//
LOCUS       DAEMAE010000441          586 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.449, whole genome shotgun sequence.
ACCESSION   DAEMAE010000441 DAEMAE010000000
VERSION     DAEMAE010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.449"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(15..455)
                     /locus_tag="KPH93_003805"
     CDS             complement(15..455)
                     /locus_tag="KPH93_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG1690514.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLEAYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYVFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KKLWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
BASE COUNT          202 a           91 c           75 g          218 t
ORIGIN      
        1 cacaaaaata aaagttaatt ttgtttaggt ggagattttt ttgctcttat atattgaata
       61 atgtcaagta agtctgcatc ttctaaaata cgatacttaa tccaacctcc attattgcca
      121 caaggataac gagtaaccca gagtttttta gcttttggag tcaaagctac caatgttttt
      181 tctactgctg gaacttgctt gtcacctatc tgaatcagaa cagtaaatgc acctttttca
      241 aaaaaaacat aaaataaatg aaaagattta tggctatatt tgtaacccca accataatta
      301 tttccaaaag gaaatcgaag ttctcttgat aaattatatt gagtttccaa gtaagcttcc
      361 aacttacaca atcgtgcata tccatcttta cctatatatt ctattatttg agacaagcag
      421 ggtttattgc ttttatcaag catacgttca tacattagaa tttacctccg tttttacttt
      481 taattatagt aaagtaatta tgacaacagt atgtcaagtt tttatatatt attcaagttt
      541 ttatgtatta ttatactaat atgtaaaagt ataaagtaat tagttt
//
LOCUS       DAEMAE010000442          565 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.450, whole genome shotgun sequence.
ACCESSION   DAEMAE010000442 DAEMAE010000000
VERSION     DAEMAE010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.450"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          209 a           59 c           60 g          237 t
ORIGIN      
        1 ctaaatatct ctttattttt ataatctttt acgacttatt tatatttttc atttttataa
       61 aaaaacaata attaagaata taactatatt ctacatgtaa aaattttttt atttaaactc
      121 aattttctga taatttgata atttttcaaa atttatagtt gacttttatt ttttactgta
      181 ttaaactaat tactatacaa aacagaaaac aaaattttca aaattattaa attaaaagga
      241 gaggtaaata tgaagacttt attaaacaaa caagtacgat tttttagtag ccaatttagt
      301 tagggtttgt acctaagtta agacaaattg tcataacata gatacaagcc ccttaggatt
      361 atgattttta aggattgtat ctatgttggc tatagtaatt gttgttaaag tttttatttt
      421 atcatcataa tatagagcca catggattat actgtgtggc ttattcgcat ttttgccata
      481 cagttatata tactgtgtgg tttttttata tcaaaattat tttttaaaat caaatttaaa
      541 aaaccattta aaatattttc aaagg
//
LOCUS       DAEMAE010000443          557 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.451, whole genome shotgun sequence.
ACCESSION   DAEMAE010000443 DAEMAE010000000
VERSION     DAEMAE010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.451"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..238
                     /locus_tag="KPH93_003806"
                     /pseudo
     CDS             62..238
                     /locus_tag="KPH93_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT          227 a           72 c          116 g          142 t
ORIGIN      
        1 aaactataat atgtaataca gccagtatat tgatataaaa aacttaggag gagttgtaaa
       61 catgggtaat ttaaaaagac agtgtgatgt ttcaagtgga agagaaaaag ctgacattgt
      121 actaaaaaat gggacaatca taaatgtatt tacagaagag ttaataacag gagatgtggc
      181 aatagtagga gacactattg ttggaattgg cgattacaag ggcaatgtag aaattgatta
      241 aaaaaatagc tacgcacact cgtgacttta gtcatgagtt agtagctaaa atagtcagca
      301 tatagggaaa cttatatgta gagataggat agaacctatc caatactact tgaattgctg
      361 gaaaccccta aagctaacca aactaaaaca taaggatgaa ataaacctaa gtgtgaaagt
      421 tacgaaagta gaaaaaattg gttagatggt ataaggttaa atcctaagta ctttaaaatg
      481 ggcaatcagc aggtaagctc cgaataggag aaacttcaac gactattcct cttgagggaa
      541 gtacactaca agctatt
//
LOCUS       DAEMAE010000444          556 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.452, whole genome shotgun sequence.
ACCESSION   DAEMAE010000444 DAEMAE010000000
VERSION     DAEMAE010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.452"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(333..449)
                     /gene="rrf"
                     /locus_tag="KPH93_003807"
     rRNA            complement(333..449)
                     /gene="rrf"
                     /locus_tag="KPH93_003807"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          191 a           76 c           75 g          214 t
ORIGIN      
        1 gttaaattat aaacattttt tgttaaatat aatgaagtta agatgatata ctgtttatga
       61 tagctataac tttttatact tttcatagtc aatattcaat gtcacaatat aattttaatt
      121 tagaaggggt catataaaac tatttatttt tatatgattt gatttgaaaa taaaaaagac
      181 ctctatgggg gatagaggtc tttaagtggg ggagattgag taatttattt ttgctatatt
      241 aagttttaca attatattat gtcccaaatt tgcaaggtct aaacataaaa aacaaaactt
      301 ttttaatttt tataaattta ctcaaaaaaa ttacgtggct acgtcctact ctcccaggca
      361 gtttcccacc aagtaccatc agcgctagag agcttaactt ctgtgttcgg aatgggaaca
      421 ggtgtatcct ctctgctatt gtaaccacat aatctttatg cttaatattt ctattaagtt
      481 tttaataatt ttttagaatc tagtaacact agtttcttta gagttaacac tctaaaaact
      541 acacatatat cttaat
//
LOCUS       DAEMAE010000445          540 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.453, whole genome shotgun sequence.
ACCESSION   DAEMAE010000445 DAEMAE010000000
VERSION     DAEMAE010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.453"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>540
                     /locus_tag="KPH93_003808"
     CDS             <1..>540
                     /locus_tag="KPH93_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1690515.1"
                     /translation="VFDYTSQTKTDLKKLNMYFLFSVFLIISSLYINIGANSRILICI
                     FVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLDNMNVLLSGSLY
                     RYEAIFLGKLMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFFVLYDYIINLVK
                     ISPERRTAFLVIVLLLILSN"
BASE COUNT          186 a           48 c           74 g          232 t
ORIGIN      
        1 gtttttgatt atacgagtca gacaaagact gacttaaaaa aactaaatat gtactttctc
       61 ttttctgttt ttcttattat ttcttcttta tatataaata taggagctaa tagtagaatc
      121 ttgatatgta tatttgtaat gttcgttttc tataagtcta actataaggt taatatatat
      181 aaatgtctgg taatctcatt tttttattgg ttaatattaa taggtataaa tggattaagt
      241 atgttattag tagcaacagt aagcaatttg gataatatga atgttttact tagtggaagt
      301 ttatatagat atgaagcaat attcttggga aagctaatgt taggtatttt actttatgtt
      361 tataaagctt cttttaaaaa ttttaaaata agtaaaaaag agatatacat atgtattcct
      421 atgatagcaa atttattgtc attttttgta ctttatgatt atattattaa tcttgtaaaa
      481 atctctcctg aaagaagaac tgctttttta gtaatagttt tgctattaat attatctaac
//
LOCUS       DAEMAE010000446          532 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.454, whole genome shotgun sequence.
ACCESSION   DAEMAE010000446 DAEMAE010000000
VERSION     DAEMAE010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.454"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>532
                     /locus_tag="KPH93_003809"
     CDS             <1..>532
                     /locus_tag="KPH93_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1690516.1"
                     /translation="IKLIPKIIRLVNKTYIFLGILTIIVYMIIESIVTHLIINAVQKT
                     KVKFIGIKMATMGFYYNLVTPFASGSQPMQIYALTKYDVSLSKSVAIVTNKTVVFQST
                     VTVFCGILILLNKSLLMEQVHSVKVLITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVL
                     INFLSKFKTFKFLDSKR"
BASE COUNT          201 a           58 c           81 g          192 t
ORIGIN      
        1 atataaaact aatacctaaa attataaggc tagtaaataa gacttatata tttcttggca
       61 tattgacaat tatagtatat atgataattg aatctattgt aactcatttg ataataaatg
      121 cagttcaaaa aacaaaagtg aaatttattg gtattaaaat ggctacaatg ggattttact
      181 ataacttagt tactccattt gcatctggga gtcaaccaat gcaaatatat gcacttacaa
      241 aatatgatgt cagtcttagt aaatcagtgg ctatagttac taataaaact gtagtatttc
      301 aaagtacagt taccgtattt tgtggaattt taattttgct aaataagtca ttactgatgg
      361 agcaagtaca ttctgtgaaa gtcttgataa cttttggaat gattatgaat gtaagtatgt
      421 tgttaggtgg actactaata gtatttagtc caaaacaagt aaaaatgata gcagaagttt
      481 tgattaattt tttatctaaa tttaaaacat ttaagttttt agattcaaaa cg
//
LOCUS       DAEMAE010000447          518 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.455, whole genome shotgun sequence.
ACCESSION   DAEMAE010000447 DAEMAE010000000
VERSION     DAEMAE010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.455"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>518)
                     /locus_tag="KPH93_003810"
     CDS             complement(<1..>518)
                     /locus_tag="KPH93_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG1690517.1"
                     /translation="FLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIICTLLV
                     IIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSLVCSV
                     GELIFSKNLGYEFPSLIYFIFILSKCLIIYAEIYMVDVRKFKVNRYALRDNLAICFIY
                     FSFIFLLNTFTN"
BASE COUNT          230 a           62 c           65 g          161 t
ORIGIN      
        1 agttggtaaa tgtgtttagt aaaaatatga atgaaaaata aataaaacat atagctaagt
       61 tatctctaag tgcatatctg ttaactttaa attttctaac atcaaccata tatatctctg
      121 cataaattat caagcactta gataagataa atataaaata tattaagcta ggaaattcat
      181 agcccaagtt tttagaaaaa atgagttctc caactgaaca aactaaactc caagaaaaaa
      241 atatattaaa aagatgatac tgcttaaaaa ttagtatggc tatacacaga tatatacaaa
      301 tataattgat tccaattggc agagagttta gagtactata attgcctgaa gaaataagta
      361 ataattgctc tagagccata attattacaa gtaatgtaca aattatcttc tcaactgtat
      421 agctataagg taaaatattt ttggttagtt ttggacaata atataaaaat aatccaaata
      481 taaagagact tacaaattgt tcactagaaa aaagaaaa
//
LOCUS       DAEMAE010000448          501 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.456, whole genome shotgun sequence.
ACCESSION   DAEMAE010000448 DAEMAE010000000
VERSION     DAEMAE010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.456"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>501
                     /locus_tag="KPH93_003811"
     rRNA            <1..>501
                     /locus_tag="KPH93_003811"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          135 a          103 c          166 g           97 t
ORIGIN      
        1 agggtgatcg gccacattgg aactgagaca cggtccaaac tcctacggga ggcagcagtg
       61 gggaatattg cacaatgggc gaaagcctga tgcagcaacg ccgcgtgagt gatgaaggcc
      121 ttcgggtcgt aaaactctgt cctcaaggaa gataatgacg gtacttgagg aggaagcccc
      181 ggctaactac gtgccagcag ccgcggtaat acgtaggggg ctagcgttat ccggatttac
      241 tgggcgtaaa gggtgcgtag gcggtctttc aagtcaggag tgaaaggcta cggctcaacc
      301 gtagtaagct cttgaaactg ggagacttga gtgcaggaga ggagagtgga attcctagtg
      361 tagcggtgaa atgcgtagat attaggagga acaccagttg cgaaggcggc tctctggact
      421 gtaactgacg ctgaggcacg aaagcgtggg gagcaaacag gattagatac cctggtagtc
      481 cacgctgtaa acgatgagta c
//
LOCUS       DAEMAE010000449          473 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.457, whole genome shotgun sequence.
ACCESSION   DAEMAE010000449 DAEMAE010000000
VERSION     DAEMAE010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.457"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KPH93_003812"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KPH93_003812"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..378
                     /locus_tag="KPH93_003813"
     tRNA            290..378
                     /locus_tag="KPH93_003813"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Leu,seq:taa)
     gene            390..466
                     /locus_tag="KPH93_003814"
     tRNA            390..466
                     /locus_tag="KPH93_003814"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:424..426,aa:Ile,seq:cat)
BASE COUNT          150 a           90 c          106 g          127 t
ORIGIN      
        1 agtttttaga gtgctaactc tatattttta aacttgtcaa aacttacaga tttttaatag
       61 ttttataagg caaaaaatcg aataaaaaaa gtattgacac aataagacaa agatgttatt
      121 ataatacttg tcttagaaaa aaagataaat gaaaatttag acaataatct ggttataata
      181 gcagagagga tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacatagcc aggtgatgtg ccgaagtggc
      301 ggaactggca gacgcacagg acttaaaatc ctgcgggact tacctctcgt accggttcga
      361 ttccggtctt cggcaccaca aattattgtg ggccattagc tcagttggtt agagcgcccg
      421 gctcataacc ggtaggtctg gggttcgagt ccctgatggc ccaccacaat aat
//
LOCUS       DAEMAE010000450          472 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.458, whole genome shotgun sequence.
ACCESSION   DAEMAE010000450 DAEMAE010000000
VERSION     DAEMAE010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.458"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..>472
                     /locus_tag="KPH93_003815"
     CDS             22..>472
                     /locus_tag="KPH93_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690518.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIALLNNEDNLKIKTIKGDPNELD
                     YININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNDDSKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRT"
BASE COUNT          213 a           37 c           72 g          150 t
ORIGIN      
        1 atctctttaa ggaggggtgg tatgaaatta aaatctaaaa tatctatact tattatattt
       61 ttgactttag caatatattt tagtatagct ttattaaata atgaagataa tttaaagata
      121 aaaactataa aaggtgaccc taatgaatta gactatataa acataaattt tggatatgct
      181 ttgaatggat atataaattc atattataca ataaatggaa gtaacaaaac caaaaagact
      241 gaattaataa aactacaaaa ggatagtaaa aacacaaata atttaattga tgtatctagc
      301 ggttcagatt taattgctca tgttggattt gaaatgaatg atgattcaaa agatataaac
      361 tttgtaatag atgagaaaaa agaagattta gaaaaaggtg tatttccatc agaaggaagt
      421 gataaagaaa ttgaatatga aaaattagat atgaataatt ttacaaggac aa
//
LOCUS       DAEMAE010000451          467 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.459, whole genome shotgun sequence.
ACCESSION   DAEMAE010000451 DAEMAE010000000
VERSION     DAEMAE010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.459"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>467
                     /locus_tag="KPH93_003816"
     CDS             <1..>467
                     /locus_tag="KPH93_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690519.1"
                     /translation="IRLKSGKFPNINSNEFISNINTNNSNQVGKFSTMSKDISILIRD
                     FGKISEVGESGILYISTQNEDTINKILKELNVDRSIFVARLHDRYVNSNLYLNPSIIR
                     DLILIILCFLATIIHYSISVSREASILRLNGYSKLDIIARVIKNILRIMILSSV"
BASE COUNT          190 a           44 c           67 g          166 t
ORIGIN      
        1 ataaggttaa agtcaggaaa atttcctaat attaattcaa atgaatttat tagtaatata
       61 aacactaata atagtaatca agtaggtaaa ttctcaacta tgagtaaaga catatcaatt
      121 ttgattaggg attttggtaa aataagtgaa gttggagaat caggaatctt atatatttca
      181 actcaaaatg aagatacaat aaataaaata ttaaaagagt taaatgtgga cagaagtatt
      241 tttgtagcaa gattacatga tagatatgtt aactcaaatt tatacttaaa tccttcaata
      301 attagagatt taatactaat tatcctttgt ttcttggcta caataataca ttattcaatt
      361 agtgtgagta gagaggcttc tattttaaga ttaaatggat actctaagct agatataatt
      421 gctagagtta taaaaaatat acttagaatt atgattttat catctgt
//
LOCUS       DAEMAE010000452          459 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.460, whole genome shotgun sequence.
ACCESSION   DAEMAE010000452 DAEMAE010000000
VERSION     DAEMAE010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.460"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(15..455)
                     /locus_tag="KPH93_003817"
     CDS             complement(15..455)
                     /locus_tag="KPH93_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG1690520.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNKNSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT          156 a           52 c           45 g          206 t
ORIGIN      
        1 ttctatatta atatttaact aatattagat aatgtatcgt ttatatcctt ttctactaat
       61 ggtttcatat tattattata aatacttata tctgcattac ttaattccat ttttatgcga
      121 ttaataagat tttctttata ctttgtaata ttaggtatgg atttaataaa cattcttgat
      181 acagaaggtt ttttatctac gatatgactt agtatactat ctatatttat ttcaatttta
      241 ttatcaatat cccactgagc atttgcagag attaataaca atccccttgt tcgaatatat
      301 gagtttttgt tatcaatcat ttctaggaat gagtcaaaaa aacaatatgt aatatttgat
      361 tttaaacttt cattttctaa gaataaaaaa gttttatatg cttcattatg attttttgaa
      421 tataatttat taataagtaa attgagattc ttcaatctt
//
LOCUS       DAEMAE010000453          433 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.461, whole genome shotgun sequence.
ACCESSION   DAEMAE010000453 DAEMAE010000000
VERSION     DAEMAE010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.461"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            143..424
                     /locus_tag="KPH93_003818"
     CDS             143..424
                     /locus_tag="KPH93_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690521.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          200 a           32 c           68 g          133 t
ORIGIN      
        1 ttaaataaat atattaaaga agaagggata taaaacaaga ttagaaaata atagtgttac
       61 tttcataata aatacatata aagatatttt taagaataaa atttattatt aaataaaaaa
      121 taaaggagag tgatttaatc gtatggcatc aaaacaaaca ccaagaaaca atttagaaaa
      181 atttttaaga ataagagtat tatcacatac aggcgataaa atacatataa agttacctgt
      241 aaattttgca aagagaatga ttgagaataa tgctttggat ttatttaatg gaaaagatga
      301 cgtagtagat ggtaaaaagg ttactgaaat attagttaaa gcatttgact atgatttaac
      361 tggagaagta gtccacttag agagaaaaaa tggagacatt attaaagttt caattgtaca
      421 atagaattat ttt
//
LOCUS       DAEMAE010000454          432 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.462, whole genome shotgun sequence.
ACCESSION   DAEMAE010000454 DAEMAE010000000
VERSION     DAEMAE010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.462"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(110..409)
                     /locus_tag="KPH93_003819"
     CDS             complement(110..409)
                     /locus_tag="KPH93_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690522.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSREINWDKVSNIRFD"
BASE COUNT          163 a           65 c           45 g          159 t
ORIGIN      
        1 attttatgct ataaatcaga agaagcactt ctataataga aagtgctcaa ctatgaataa
       61 gattaataat atatttgcct tatatataga cttttataaa tattaaagat taatcaaatc
      121 tgatgtttga aactttatcc caattaatct cccttgaata gtcttctgga tgtgataatc
      181 taactatatg agttgaatta tcataactgt aataaattgt agtagtacca tcaactggat
      241 tctttttgta tccttgatac acagcactat ttggtacttt cttttcaaca aaactatatg
      301 cagatgaagt atcaatagtt tctattttat taggaaatat aaacatataa aaagctacta
      361 ctataaaaat acatatgcat gtaatactat accatttaat ccatttcata aaattcctcc
      421 tactttacca aa
//
LOCUS       DAEMAE010000455          415 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.463, whole genome shotgun sequence.
ACCESSION   DAEMAE010000455 DAEMAE010000000
VERSION     DAEMAE010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.463"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            9..>415
                     /locus_tag="KPH93_003820"
     CDS             9..>415
                     /locus_tag="KPH93_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1690523.1"
                     /translation="MKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVYKNN
                     KETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLENAYEKFFKEKTGFPKFKSK
                     KTNRFSYKTNFTNGNIMYCGQHIKLPKLGMVKI"
BASE COUNT          189 a           45 c           55 g          126 t
ORIGIN      
        1 tgatagcagt gaaaaaagct tataagttta gaatgtatcc aaataaaaaa cagcaagagt
       61 taattaacaa aacttttgga tgttgtagat ttgtatataa taaatatctt gctaaaagaa
      121 tagaagttta taaaaacaat aaagaaactt ttacttataa acagtgtagt tctgatttga
      181 ctaatttaaa aaaagaatta aattggctta aagaacctga caaattttca ctacaaaacg
      241 ccttaaaaga tttagaaaac gcatatgaaa aattctttaa agaaaagaca ggatttccta
      301 aatttaaatc aaagaaaact aatagatttt catataaaac taactttaca aatggaaaca
      361 ttatgtattg tggtcaacat ataaaactac ctaaacttgg tatggttaag ataag
//
LOCUS       DAEMAE010000456          399 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.464, whole genome shotgun sequence.
ACCESSION   DAEMAE010000456 DAEMAE010000000
VERSION     DAEMAE010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.464"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>399
                     /locus_tag="KPH93_003821"
     CDS             <1..>399
                     /locus_tag="KPH93_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690524.1"
                     /translation="LYATSDSSENNKEHSSTIDIIKINPENKKIKTIKSINLSNYFSE
                     KYQDIETIGVSYASKYEDKMYVLVNVLNTSIGDNSNSKFHLLEYNLKENKESIMELKT
                     DSKMALAKVKLEDNKLYMVMSNDYDNSSIT"
BASE COUNT          186 a           35 c           58 g          120 t
ORIGIN      
        1 tactttatgc tacaagtgat tcatcagaaa ataacaaaga acattcaagt actatagata
       61 taataaaaat taatccagaa aataaaaaaa tcaagactat aaaaagtata aatttaagta
      121 attatttttc cgaaaaatac caagacatag agactatagg tgtaagttat gctagcaaat
      181 atgaggataa gatgtatgtt ttagtaaatg tactaaatac aagtataggt gataatagta
      241 attctaaatt tcatttactt gagtataatc ttaaagaaaa taaggaaagc ataatggaat
      301 taaaaactga tagcaaaatg gctttagcaa aagtaaagtt agaagataat aaattatata
      361 tggtaatgtc aaatgattat gataatagtt caattacag
//
LOCUS       DAEMAE010000457          395 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.465, whole genome shotgun sequence.
ACCESSION   DAEMAE010000457 DAEMAE010000000
VERSION     DAEMAE010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.465"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      <1..74
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          159 a           51 c           47 g          138 t
ORIGIN      
        1 ctagagaaat ctagtgacgc aaagctatag ggactaaggt ttgtatacat gaactatgtc
       61 agccagttgc caaaaagagt cctaggtgta ttgtatacct aagaaaagtc tagaatgact
      121 gcctttttgg cagtcatttt gttatggaaa aattacttta ttttagtaga aaacacatat
      181 aataacatat tttaccaatt ataacataaa ataaagaaaa attatctcaa aaagttacta
      241 ttttacaaaa aatgacttct ttattcattt aaattaatcg catttccatt acattaatac
      301 aataatgtat cattttagta agaaaactaa tattttttca atgaagtccc atgaaaatat
      361 aaaaaaattt taatatgtat ttaaatattt cttca
//
LOCUS       DAEMAE010000458          394 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.466, whole genome shotgun sequence.
ACCESSION   DAEMAE010000458 DAEMAE010000000
VERSION     DAEMAE010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.466"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            10..315
                     /locus_tag="KPH93_003822"
     CDS             10..315
                     /locus_tag="KPH93_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690525.1"
                     /translation="MPSIIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     IGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          150 a           37 c           60 g          147 t
ORIGIN      
        1 ggggtcaaaa tgcctagtat aattatggaa gttgtaggag tactatttat agctattgga
       61 ttaatcttga cattcattga aataaaagga aatttccctt ctttttatgg ttttggtcaa
      121 aattctatag ggaaaaatgc tataggtatt atagcagcag gaattgcttt aattttcttt
      181 agaaatggca tagatagcat agattctata gctacttatg gtgtattttt agctatagaa
      241 cttgttgtaa tagttgcata tttcttaata agaaaaaaat ttactaaaga agataaacat
      301 ttaagaacta attaactaaa ttatctacta taaaattgga aaattaaaaa caaataaaag
      361 tttgttttta attatttaaa atccaaattt gatt
//
LOCUS       DAEMAE010000459          368 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.468, whole genome shotgun sequence.
ACCESSION   DAEMAE010000459 DAEMAE010000000
VERSION     DAEMAE010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.468"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..>368
                     /locus_tag="KPH93_003823"
     CDS             58..>368
                     /locus_tag="KPH93_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690526.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     Q"
BASE COUNT          170 a           40 c           40 g          118 t
ORIGIN      
        1 tattagttaa gtatatattt ttaataaata tttttataat tagaaggaag tggttttgtg
       61 caaatagaac aactcgattt agaaacgaga aataaaatat actgctatac taaaaaaatt
      121 cttagaaaat atcaaaaagg cataacatct ggaaaattaa cagcagataa atttgcagat
      181 aatatattgt ctcaagattt tatcagtagt atacttaatg aaaaaatagt caatgagaca
      241 aactttaaga tatcttatag aaactatata gaaactttaa taaatattca aaatgaaaat
      301 ttatctaact taagaaaaaa atctactaaa actgcaaaat gtttcaatat ctctcaaata
      361 acccaatt
//
LOCUS       DAEMAE010000460          366 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.469, whole genome shotgun sequence.
ACCESSION   DAEMAE010000460 DAEMAE010000000
VERSION     DAEMAE010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.469"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          144 a           48 c           26 g          148 t
ORIGIN      
        1 cacatatatg atataaattc aacatccata ttgtgagtaa tattgttata taaaatatag
       61 gatgtataca taaatataat tattgaactt gttatcaatc ccactactga atttgataaa
      121 taagttaaca ttattataaa aaaaattatt gctagcatta tgaacacatt cagtttattt
      181 tctgtcctaa tcgatagtat tactattcca acaattaaaa atataaaaac gagcaaagct
      241 aatattaata aatctatttt tttatttatc ctattcattt tttataaatt tacacctcca
      301 attattctat taagttgccc attttttcta attattatta aaatcattat acaaataagt
      361 tttcat
//
LOCUS       DAEMAE010000461          358 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.470, whole genome shotgun sequence.
ACCESSION   DAEMAE010000461 DAEMAE010000000
VERSION     DAEMAE010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.470"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          176 a           30 c           42 g          110 t
ORIGIN      
        1 attaataata attacatata ataataatta tatgattaat aataattgca tatagtatta
       61 ttataattgg aaattactat taataatagt tatatcttta aaaataaagt cacaatagta
      121 tgacaaaggg tgaataatca ataaattttt aaaaaaaaga aaaaaatagt tgaccatatt
      181 atgagaagat gttattataa taaatgtgct caacaagaag taacaaaata acaagcacaa
      241 agaagttaat tgaatgaaat gaactttgaa aattaaacag taggttaatt tataaaacaa
      301 gaaacaaacc ataaagccag atattttgat aacaatagta tctgagcctg ataaactt
//
LOCUS       DAEMAE010000462          354 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.471, whole genome shotgun sequence.
ACCESSION   DAEMAE010000462 DAEMAE010000000
VERSION     DAEMAE010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.471"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>354)
                     /locus_tag="KPH93_003824"
     CDS             complement(<1..>354)
                     /locus_tag="KPH93_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBG1690527.1"
                     /translation="KRNLRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALFFI
                     WPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPISIVLIILSVIILAFYAFFELTSVII
                     CFNKSIKYEKIGLFE"
BASE COUNT          148 a           36 c           32 g          138 t
ORIGIN      
        1 gttcaaataa ccctattttt tcatatttta tcgatttatt aaaacaaata attacagagg
       61 taagttcaaa aaatgcataa aaagctagta ttataactga tagtattatc agaactattg
      121 atatgggatt ggttaaaact tttataaaat tatccatatt taggtagact atatgtaaac
      181 tttttattaa tatatcaaat atatatctaa tagatggcca tataaaaaat aaggctaatc
      241 ctttatgaaa taattcaaat aaaattagtg tactaaaatt atattttatt ataaaaacaa
      301 gtttacttaa aatattttta cctgtaaata taaaattcct taaatttctc tttt
//
LOCUS       DAEMAE010000463          348 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.473, whole genome shotgun sequence.
ACCESSION   DAEMAE010000463 DAEMAE010000000
VERSION     DAEMAE010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.473"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          143 a           29 c           47 g          129 t
ORIGIN      
        1 gatataacat ataaaaccat tataatatat gttaaagata agaatgtata aggtggtaaa
       61 tttgtataaa aaagaatatg agttgtaaaa tattaaataa tattgtacta attggaataa
      121 ttttaacttt attatagatt attttaatac cattagtact gatgatgtat tttaaaagtg
      181 gtataagcat tacaaatacg aatatgccaa ctgtaatatg taatataagt agctatatac
      241 atatgtgttg tcccatatat tttatcattg tttatattaa aaagcttatg taaaacagaa
      301 gaaagagagg gagacactat tatatccctc ttttttctat agtaatta
//
LOCUS       DAEMAE010000464          330 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.474, whole genome shotgun sequence.
ACCESSION   DAEMAE010000464 DAEMAE010000000
VERSION     DAEMAE010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.474"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>330)
                     /locus_tag="KPH93_003825"
     CDS             complement(<1..>330)
                     /locus_tag="KPH93_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690528.1"
                     /translation="SIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNE
                     YEIIIDTKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWK
                     LSESFKGK"
BASE COUNT          108 a           51 c           26 g          145 t
ORIGIN      
        1 cttaccttta aatgattctg ataatttcca agaactataa ttcttatctt tttgaataca
       61 aggatataat aatatacatc tttttgcatt ttcatatgct gttatatatg catacatctg
      121 gtatatatca cttgacctat aaaaaacatt tgaatccact tcaatagctt tccatttagt
      181 gtcaattata atttcatatt cattttttaa atcatataaa actatatctg gtcttaaatt
      241 aaagtttttc ttacctgttt gttcattttt caaaagaaat ttactcttat cttgtatata
      301 tgtttctcta aatgaattat cccatatact
//
LOCUS       DAEMAE010000465          326 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.475, whole genome shotgun sequence.
ACCESSION   DAEMAE010000465 DAEMAE010000000
VERSION     DAEMAE010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.475"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          143 a           16 c           46 g          121 t
ORIGIN      
        1 tgttttgtag aatgataaaa ataaggatta agtgtaataa aatagaaaac aagtatgtta
       61 attttaagta tgtgctttaa agtttatcca agttaaaaat ttaagatatc ttttcagtat
      121 aatggaaaaa atgtcaaaat attgaaatag ggaaaaaata gtgatataat aagaaaaaat
      181 agagaaaaaa agtaaccctt agttgtaaaa aagttactat ttattgaaaa tttagatatt
      241 tttctaaaga ttatatatgt taatagttaa atttatgaac attttttgtt tttatgttag
      301 gatatttctt agaaatgaaa tacttc
//
LOCUS       DAEMAE010000466          318 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.476, whole genome shotgun sequence.
ACCESSION   DAEMAE010000466 DAEMAE010000000
VERSION     DAEMAE010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.476"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(179..>318)
                     /locus_tag="KPH93_003826"
     CDS             complement(179..>318)
                     /locus_tag="KPH93_003826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690529.1"
                     /translation="GVDYKGENTKAAAFIVNGNSVPTRTLKTNLTNVAKSVINLLKIN
                     E"
BASE COUNT          109 a           45 c           24 g          140 t
ORIGIN      
        1 tatacctctt taccattaat tattttaaaa tagtaaacat aaatttagtt actccatata
       61 aaaaacctct taattaatct acttctaaat ttatatcaat caagaggttt acacataatt
      121 taatttttaa tctaatatta aattaattaa aaatataagt atcaatgttt atccattctt
      181 actcatttat ttttaataaa ttaattacag attttgctac atttgtaaga tttgttttta
      241 atgttcttgt aggaacgcta tttccattta ctataaatgc tgctgctttt gtgttttcac
      301 ctttgtagtc aactccta
//
LOCUS       DAEMAE010000467          309 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.477, whole genome shotgun sequence.
ACCESSION   DAEMAE010000467 DAEMAE010000000
VERSION     DAEMAE010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.477"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>309
                     /locus_tag="KPH93_003827"
     CDS             <1..>309
                     /locus_tag="KPH93_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1690530.1"
                     /translation="IIFMSIKYFKLSIEFKPKDMVLIGLPILSNIVSLLLIFGYNLKN
                     NFVSDDNIFVIVLSTLLIVSSSIIILIVIGKIVQDDKVKLEYELINERIKIDYKSYES
                     "
BASE COUNT          122 a           20 c           46 g          121 t
ORIGIN      
        1 ttataatatt tatgtcaatt aagtatttta aattatctat agaatttaaa cctaaggata
       61 tggtgcttat aggtttacct atattatcaa atatagtaag tttgttatta atatttgggt
      121 ataatcttaa gaataatttt gtaagcgatg ataatatatt tgtaattgta ctttcaactt
      181 tattgattgt gtcatctagc ataataattt taatagtaat agggaaaatt gtacaagatg
      241 ataaagtcaa gttagaatat gaactgataa atgagagaat aaaaatagat tataaaagtt
      301 atgagtcta
//
LOCUS       DAEMAE010000468          295 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.478, whole genome shotgun sequence.
ACCESSION   DAEMAE010000468 DAEMAE010000000
VERSION     DAEMAE010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.478"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..268)
                     /locus_tag="KPH93_003828"
     rRNA            complement(<1..268)
                     /locus_tag="KPH93_003828"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           71 a           78 c           52 g           94 t
ORIGIN      
        1 ggctaatccg cgttcgctcg ccgctactta cggaatcgaa tttctttctc ttccttcagg
       61 tacttagatg tttcagttcc ctgagttccc ctcattaagc tatgtattca cttaatgata
      121 cttagacatt actctaagtg agtttcctca ttcggaaatc ttcggatcaa agtttacgtg
      181 caactccccg aagcttatcg cagcttatca cgtccttcat cggctcctag tgccaaggca
      241 tccgccctgc acccttaata acttgaccag ttaaaaaggt ttgatagatt agaag
//
LOCUS       DAEMAE010000469          293 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.479, whole genome shotgun sequence.
ACCESSION   DAEMAE010000469 DAEMAE010000000
VERSION     DAEMAE010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.479"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>293
                     /locus_tag="KPH93_003829"
     rRNA            <1..>293
                     /locus_tag="KPH93_003829"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           81 a           54 c           96 g           62 t
ORIGIN      
        1 catacgggtt gaagcatgac cgtaaggaca tgtggacagt atgggagtga gaatgttggc
       61 atgagtagcg agatgtgggt gagaatccca caggccgtaa acccaaggtt tccaggggaa
      121 ggttcgtccg ccctgggtta gtcgggacct aagctgaggc cgaaaggcgt aggtgatgga
      181 caacaggttg atattcctgt actaccgata accgtttgag agaagggatg acacagtagg
      241 ataagctaag cacactgttg gttatgtgtg cccaagcatt gaggcagtca aag
//
LOCUS       DAEMAE010000470          285 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.480, whole genome shotgun sequence.
ACCESSION   DAEMAE010000470 DAEMAE010000000
VERSION     DAEMAE010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.480"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            148..264
                     /gene="rrf"
                     /locus_tag="KPH93_003830"
     rRNA            148..264
                     /gene="rrf"
                     /locus_tag="KPH93_003830"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          101 a           42 c           54 g           88 t
ORIGIN      
        1 agtttttaga gtgttaactc tatgtaaagt actaacttca attgatgaag tatatatcgt
       61 aaaaacaatt actatttagt acttcaaaac tattgacatt taaaaataaa gatgttaata
      121 taatacttgt cttataaata ggcaatatgt ggttataata gcaaagagga tacacctgtt
      181 cccattccga acacagaagt taagctcttt agcgctgatg gtacttggtg ggaaactgcc
      241 tgggagagta ggacgtagcc acgtgaatat tccaaggtag ctcaa
//
LOCUS       DAEMAE010000471          284 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.denovo.481, whole genome shotgun sequence.
ACCESSION   DAEMAE010000471 DAEMAE010000000
VERSION     DAEMAE010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.denovo.481"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>284)
                     /locus_tag="KPH93_003831"
     CDS             complement(<1..>284)
                     /locus_tag="KPH93_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1690531.1"
                     /translation="VDGQSFEENLIKDIIENLKMKNNSDIEGYKQSLKELDEYIAKWV
                     HEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMF"
BASE COUNT           90 a           47 c           23 g          124 t
ORIGIN      
        1 tcaaacatag tgttgcttct actactatac aaaatagagt ttactaaaaa atttatcttt
       61 gcaacctgat tcttaatatc caaactatct tctatgctgc ttaatctatc tgtaattata
      121 cttaaagaag atattggaat ttttatttca tgaacccatt tagctatgta ctcatctaat
      181 tcttttaatg attgtttata accctctatg tcactattat ttttcatttt taaattttct
      241 ataatatctt ttattagatt ttcttcaaaa gattgtccat caac
//
LOCUS       DAEMAE010000472        24440 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.038, whole genome shotgun sequence.
ACCESSION   DAEMAE010000472 DAEMAE010000000
VERSION     DAEMAE010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.038"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..1363
                     /locus_tag="KPH93_003832"
     CDS             167..1363
                     /locus_tag="KPH93_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1690532.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            1376..1510
                     /locus_tag="KPH93_003833"
     CDS             1376..1510
                     /locus_tag="KPH93_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG1690533.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            1511..1732
                     /locus_tag="KPH93_003834"
     CDS             1511..1732
                     /locus_tag="KPH93_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690534.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            1936..2712
                     /locus_tag="KPH93_003835"
     CDS             1936..2712
                     /locus_tag="KPH93_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690535.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            2797..3093
                     /locus_tag="KPH93_003836"
     CDS             2797..3093
                     /locus_tag="KPH93_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1690536.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            3012..3515
                     /locus_tag="KPH93_003837"
     CDS             3012..3515
                     /locus_tag="KPH93_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1690537.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            3533..4036
                     /locus_tag="KPH93_003838"
     CDS             3533..4036
                     /locus_tag="KPH93_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1690538.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            4154..4552
                     /locus_tag="KPH93_003839"
     CDS             4154..4552
                     /locus_tag="KPH93_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1690539.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            4530..6980
                     /locus_tag="KPH93_003840"
     CDS             4530..6980
                     /locus_tag="KPH93_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1690540.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            6980..9175
                     /locus_tag="KPH93_003841"
     CDS             6980..9175
                     /locus_tag="KPH93_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1690541.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            9172..10179
                     /locus_tag="KPH93_003842"
     CDS             9172..10179
                     /locus_tag="KPH93_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG1690542.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            10195..11106
                     /locus_tag="KPH93_003843"
     CDS             10195..11106
                     /locus_tag="KPH93_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1690543.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            11227..11604
                     /locus_tag="KPH93_003844"
     CDS             11227..11604
                     /locus_tag="KPH93_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1690544.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            11605..12468
                     /locus_tag="KPH93_003845"
     CDS             11605..12468
                     /locus_tag="KPH93_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690545.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            12465..13109
                     /locus_tag="KPH93_003846"
     CDS             12465..13109
                     /locus_tag="KPH93_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1690546.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            13122..13313
                     /locus_tag="KPH93_003847"
     CDS             13122..13313
                     /locus_tag="KPH93_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HBG1690547.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            13518..13706
                     /locus_tag="KPH93_003848"
     CDS             13518..13706
                     /locus_tag="KPH93_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG1690548.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(13814..14173)
                     /locus_tag="KPH93_003849"
     CDS             complement(13814..14173)
                     /locus_tag="KPH93_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1690549.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            14339..14554
                     /locus_tag="KPH93_003850"
     CDS             14339..14554
                     /locus_tag="KPH93_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBG1690550.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            14571..14843
                     /locus_tag="KPH93_003851"
     CDS             14571..14843
                     /locus_tag="KPH93_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBG1690551.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            14845..14994
                     /locus_tag="KPH93_003852"
                     /pseudo
     CDS             14845..14994
                     /locus_tag="KPH93_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            15055..15396
                     /locus_tag="KPH93_003853"
                     /pseudo
     CDS             15055..15396
                     /locus_tag="KPH93_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            15521..16258
                     /gene="erm(B)"
                     /locus_tag="KPH93_003854"
     CDS             15521..16258
                     /gene="erm(B)"
                     /locus_tag="KPH93_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG1690552.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            16404..16688
                     /locus_tag="KPH93_003855"
     CDS             16404..16688
                     /locus_tag="KPH93_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBG1690553.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            16791..17669
                     /locus_tag="KPH93_003856"
     CDS             16791..17669
                     /locus_tag="KPH93_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG1690554.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSTTIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLAYSMQ"
     gene            18117..18533
                     /locus_tag="KPH93_003857"
     CDS             18117..18533
                     /locus_tag="KPH93_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1690555.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            18540..18788
                     /locus_tag="KPH93_003858"
     CDS             18540..18788
                     /locus_tag="KPH93_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1690556.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            19039..19440
                     /locus_tag="KPH93_003859"
     CDS             19039..19440
                     /locus_tag="KPH93_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1690557.1"
                     /translation="MIRPHGAVLPTRMGIVEILTELSKAVCLLIIDSFSNKIYLLFLF
                     RCVIMTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAEQLDISKKVAY
                     SLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            19459..20904
                     /locus_tag="KPH93_003860"
     CDS             19459..20904
                     /locus_tag="KPH93_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HBG1690558.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
     gene            complement(21113..21189)
                     /locus_tag="KPH93_003861"
     tRNA            complement(21113..21189)
                     /locus_tag="KPH93_003861"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21153..21155),aa:Arg,seq:cct)
     gene            complement(21257..22951)
                     /gene="fdhF"
                     /locus_tag="KPH93_003862"
                     /pseudo
     CDS             complement(21257..22951)
                     /gene="fdhF"
                     /locus_tag="KPH93_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23202..24260)
                     /locus_tag="KPH93_003863"
     CDS             complement(23202..24260)
                     /locus_tag="KPH93_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1690559.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         8205 a         4193 c         4898 g         7144 t
ORIGIN      
        1 taagccacgc cagcaggtaa aacccctcgg tatctaacag aggggtacgt ttgcaaatag
       61 acaatagacg aaaaacatag gaaacataag gcttctggca tgatttccca gtaaaaatcg
      121 gctgtgaagt cgccttaaaa aacttcacac ttgacagatt gggggtatgg ttctgaatga
      181 agaacaatgg ataaaagaat tacgggagaa acggattgct tacggtatct cacaaggcag
      241 gctggcggtg gcttctggta tcacaaggga atatctcaac aagatagaaa gcggaaaaat
      301 gaagccgtca aaggaacttc tggaaactct gcataaagaa ctggcaaggt tcaatccaga
      361 agcaccgctt accatgctgt ttgattatgt gaaaattcgt tttcccacgc tggatataca
      421 gcacatcatc aaagatatat taaaactgaa tatcaactat atgctccatg aagattacgg
      481 acattacagt tatacggagc attattcttt aggggacatc tttatctata cgtcggctga
      541 cgaagaaaaa ggtgtccttt tagagttaaa ggggcgtggt tgccgacagt ttgagagtta
      601 cctgctggca cagcaaagaa gctggtatga ctttctcatg gacgcactca tagacggtgg
      661 cgtgatgaag cgtatcgacc ttgctatcaa cgaccatacg ggcattttgg atattccaga
      721 gcttgcggaa aaatgcagaa aacgggaata tatcggaaag tccagaagtt ataagtttta
      781 ccagtcgggc gagcttatca agcacagcga ggacgacaga gaatatatgg gacgtaccct
      841 ttatcttggt tcgctgaaat ctgatgtata tttctgtatc tatgaaaagg actatgagca
      901 gtatgtcaag ttagggacac ctctggaaga agccgatatt atcaaccgtt ttgagatacg
      961 gcttagaaat gaacgtgcct attatgcagt acgggatttg ctgacgtatt acgacgcaga
     1021 gcagaccgcc ttttctatca tcaatcagta tgtacggttt gttgatgaag aaccagacaa
     1081 gcggaaaaat gactggaaac tcaatgaccg ctgggcttgg tttatcggcg ataatcggca
     1141 aagtctgaaa ctgacgacaa agccagaacc ttatacctta gatcgtacat tgcgttgggt
     1201 acaaaggcag gtagcaccga ccttgaaaat gctgaaaaag attgataagg gaaacggtac
     1261 agattacatg gagacaatcg aacagcaggc aaagctcaca gaaaagcatg aaatgataat
     1321 caaacagcag acgacccctg caaaagattt agtagaaatt taggagtgat gaaaaatgtg
     1381 ggtattatta aaagacttcc tgctggtatc tatgggaatg ggtatcggca tagtcttgat
     1441 gtgtattttg aatgtcggca aagaagctga ctataaaatg aaacaattag aagaaagcga
     1501 ggacaattaa atgaatttcg gacaaaattt gtatcaatgg tttttaagta acgcacagag
     1561 cttagtgctt atggcgattg tggttatcgg tatctactta gggttcaagc gtgagttttc
     1621 caaacttatc ggcttcttgg tagttgcctt gattgctgtc ggtttggtat tcaatgctgg
     1681 cggtgtgaaa gatgtattgt tagagctgtt caataagatt atcggtgcat aaaattttat
     1741 tgtggtactg atatgcgaat gttataattt gaatgttgta ttacataaaa cgctgtactt
     1801 accagaaaaa tctatgaatt ttgtatatta tacaaggttg caacttgaag ttgctaatgt
     1861 gcatttcaaa aaatatagaa tataacatta tggcttgata aaatttattc atcaaatcat
     1921 aggaggtatc acacaatgag tttaggagaa aaaatcttca agttaagaaa agaaaaagga
     1981 atatcgcaag aagcactagc tgaacaaata ggcactacta ggcaggcaat tagtaaatgg
     2041 gaaaacaatc aagggtttcc agaaacagaa aagcttttac aattatcaaa catttttgaa
     2101 gtatctacgg attatctttt gaaagacgaa aagaatgtaa agaatagtga agaacgagga
     2161 tattatgtaa gcaaggaaat gtcggtaggg tttatcgcta atcagaaaaa attaggactt
     2221 tatcttgctt taggggttat gctttgcatt tttgctggaa taccatatac cttgttttta
     2281 gataatatga cttggcgtta tttaggtatg gcattttgct ttgtggcagg aattatattt
     2341 tttgtcgtag caatttttta tgaaaaacaa gaatacaaca ttttaaggca agaaccttta
     2401 ctacttgatt atgaatatct aaaagaattg aaaagcgaat acacttcaaa aaagaaaaaa
     2461 tatgtaatag ttgctgttcc ttgtactgtt ctctttatag caggactaat tttctttcta
     2521 tttactgtga aaggctatat ttcatggaca gaatatcatt cctttgcatt tttcggtctt
     2581 gctgttggaa tgttgggttt tgtatataca atcaatgtta tggaaaccta tgaactatta
     2641 gtgaaaaacg agcaatattc aacgagtttc tttttcaaac taaaaagaaa gattaaaaag
     2701 agattaggat aagctataat ttaatattca cacaaagcag ttagtcttat gattgactgc
     2761 ttttttcata ccaaaatttc agattggagt gattacatga acgatatttt taaggatatg
     2821 caggcaaaag tcggctgtga atacatttct gacctgccct cttacaagcg taaggtgtgg
     2881 cgtgaaatga aacggctgag ccctgccgat tatgaagaaa gacaattaga agatttttca
     2941 aagtttgtgt ttggtatgcc gtaccagacc ttaaaagatg tgatgaaaca acagaaagga
     3001 cgtgaagaac aatgcaggaa acaagggtgc tggtggaaac gagagaaaca actggcgaag
     3061 aaacaacatc atactggttc gacctgccga tagatgttgc cgagtttgaa gaaaagttag
     3121 gtgtcggtgc agaaagcacg gcgtaccgca ttatcgaaaa ggtgttgcct tatgctgatg
     3181 aagtccacga acatacaagc gtgtaccagc ttaacgaatt agagtttatg taccgccagc
     3241 tttcaagcga tatgcaggaa gaatatgtat cactacttac ggtgtttgag aatttagaag
     3301 cactttatat ttgcaggaac gtgattacgg tttatcccga ctgcaaaagc atgatagatg
     3361 ttgcaaggca aaagctgatg aatgactcga cgttcaaaca tttatccgag gactgtcaag
     3421 aatactactt tgactttgaa gcctacgctt ctcacttgca ggaacacggg aaattcttag
     3481 taacggaaca cggtatcttt gaactaccag agtaggaaat gaggtggtgc ttatgataga
     3541 tgatatggcg gtttacattg ctaatctcgg taaatacaat gagggttatt tagtcggtgc
     3601 ttggttcacg ttccccattg acgaggaaga tgtaaaagaa aaaatcggct tgaatgaaca
     3661 gtatgaggaa tacgctatcc atgataccga caacttcccc attgcgattg gcgagtatgt
     3721 ttccattgaa gaactcaatg agatgtatga aatgatagag gaacttcccg actatatcgt
     3781 agagtgtctg gacgaattta tcagtcacta cgggacgctg gaagaagtcg tggaacacaa
     3841 ggacgatatt tattattatc ccgactgtga aaccatgaca gatgttgcct attactacat
     3901 agacgaattg caggcacttg gggacattcc acccagctta cagaactaca ttgattatga
     3961 agcctacggg cgagatttgg atatgggcgg ttgctttatt gaaacaagcc gagggatatg
     4021 cgagatacca tattaacgct ggaagatcag caacggcatg gttgctactg acagcgtatt
     4081 tctcttttaa gggacagtct gaaaatggct gtccttttct ctttgaagaa cttacgaaag
     4141 gagctgaaag aacttgaaaa agattaaatc ttatacgggt atctggaacg tggaaaaagt
     4201 cttgtatgca atcaatgact ttaacctgcc ttttcccgtt acctttacac agattacatg
     4261 gtttgtgatt acggaattta tcatcattct gtttggggat attcccccac tttccatgat
     4321 tgagggagca tttctcaaat acttcggtat tcccgttgct ctcacttggt ttatgtcgca
     4381 gaaaaccttt gacggaaaga agccgtacag ctttttgaaa tcacagataa cctatgccct
     4441 gcgacctaaa atcacttatg cgggaaaagc cgtaaaactg cataagcaga ccttgaatga
     4501 aacaatcacg gcagtaagga gtgtgaacta tgttcccgat aaaatatatt gacaataacc
     4561 ttgtctggaa caaggacaat gaggtgttcg cttactatga gctgataccg tacaactatt
     4621 cttttctatc cgcagagcag aaatttatcg tgcatgacag tttccgacag cttatcgcac
     4681 agtcccgtga gggtaaaatt catgcgttgc agattgctac ggaaagctca atccatagta
     4741 tgcaggagca gtcaaagaag ctggtaactg gaaaattaaa ggaagttgcc tgccagaaaa
     4801 tagacgaaca gaccgaagcg ttagtatcta tgattggtga caatcaagtg gactaccgct
     4861 tttttcttgg ctttaagctc atggttacgg aagaacagct caatctgaag aacataaaaa
     4921 aatcggcgtg gctgacgttc acggaatttc tccatgaagt gaaccacacg ctgatgaatg
     4981 attttgtttc catgccgaat gatgaaatca accgttacat gaaaatggaa aagttactgg
     5041 aaaataaaat ctcccgtcgc ttcaaggtgc gtcgcttgga aatcaatgat tttgggtatc
     5101 tcatggaaca tctttacggc aggaacggta tcgcctatga agattatgag tatcagttac
     5161 caaagaagaa attgaacaaa gaaacgctga taaaatacta cgaccttatc cgtccgacaa
     5221 ggtgtgtgat tgaggaaagc cagcggtatt tacgattgga acatgaggac aaggaaagct
     5281 atgtgtccta ctttaccgtc aatgcgattg tcggggagct tgattttccg tcaagtgaaa
     5341 tcttctattt ccaacaacag caattcacat tccccgttga tacttctatg aatgtagaaa
     5401 tcgtggagaa tcgaaaggct cttaccacag taagaaataa gaaaaaggaa ttgaaagatt
     5461 tggataatca cgcctatcaa gcaggaagtg aaaccagttc aaatgtggtg gacgcattag
     5521 acagcgtgga tgagctggaa acggacttag accagacaaa agaaagtatg tataagctct
     5581 cttatgtagt gcgtgtatcg gctgatgatt tggacgaatt aaaacgccgt tgtgatgaag
     5641 tcaaggactt ttacgacgac ctcaatgtaa agctggtgcg tcctgctggg gatatgctgg
     5701 ggcttcattc tgaattttta cctgccagca agcgatatat caatgattat gtgcagtatg
     5761 taaaatcaga ttttttagct gggcttggct ttggtgcgac acagcagtta ggagaaacta
     5821 cgggtatcta tatgggctat tccgttgata cgggaagaaa tgtgtacctg caaccgtccc
     5881 ttgccagtca aggtataaaa ggtacagtta ccaacgccct tgcgtctgct tttgtcggtt
     5941 cgcttggcgg tggaaagtcg ttctgtaaca atcttctggt gtattattcg gttctctttg
     6001 ggggacaggc ggttatctta gacccaaaat cagagcgtgg caactggaag gaaacacttc
     6061 cagaaatcgc acatgaaatc aatatcgtaa acttaaccag cgataaagga aatgcaggac
     6121 ttcttgaccc attcgtgatt atgaagaatg taaaagacgc tgaaagtctg gcaatcgaca
     6181 tcttaacatt ccttacgggt atttcctcta gggacggcga aaaattcccc gtacttcgta
     6241 aagcggtacg ctccgttacc cagagcaaca gtcggggctt gctccatgtg atagacgagc
     6301 tacgccgtga agatacgccc atatcaagaa atatcgcaga ccatatcgac agcttcacgg
     6361 actacgactt tgcacatctg ctgttttcag acgggacggt agaaaatgct atcagtctgg
     6421 ataaccagct caacatcatt caagtagctg accttgtact gccagataaa gataccactt
     6481 ttgaggaata cacgaccatt gaattattat cggtttctat gctgattgtg attagtacct
     6541 ttgcccttga ttttatccat tcggacagaa gtatttttaa gattgtagac cttgacgaag
     6601 cgtgggcgtt cttaaatgtg gcacaaggag aaacgctctc taacaagctg gttcgtgctg
     6661 gacgagctat gcaggcaggc gtttattttg ttacccagtc tgcctatgat gtttcaaagg
     6721 aaagtctgaa aaacaatatc ggcttgaagt ttgcctttcg ttctactgac attaacgaga
     6781 taaaacagac cttagagttt tttggtatcg acaaggacga cgagaacaac cagaaacgct
     6841 tgcgtgattt agagaacgga caatgcttat tgcaggactt atacggacgt gtcggtgtgg
     6901 tgcagataca ccccgtcttt gaagaactgc tacacgcctt tgataccaga ccgcccgtac
     6961 agagaaatga ggtggagtga tgaaagaaag gataaaaggt gcgttcacaa aaaagaagat
     7021 tttccacttt ctcaaaatgg ttctgttcgt ggtggcactc tcccttatcc tgctttcact
     7081 tctggggacg gtggctcatg cgacggggct tgtggacgat accataaatg cagaaaatct
     7141 ttactcaaaa tatccgctgt cgaattacca gcttgatttt tatgtggata atagctggtc
     7201 gtggcttccg tggaactggc tggacggtat cggaaaatcg gtgcagtatg ggctttactg
     7261 cattaccaac tttgtctgga cgataagcct ttatttaagc aatgccacgg gctatgtggt
     7321 gcaggaagcc tataaactgg acttcattaa cgatatggca gacagtatcg gaaaaagcat
     7381 acagaccatt gcaggcgtaa cacagaacgg tttttcttct acgggcttct atgttggttt
     7441 cctgcttctc attatcttag tggtgggact ttatgttgcc tatacgggac tgataaaacg
     7501 ggaaaccagc aaggcacttc atgccgttat taactttgtg gtggtgttcg tgctgtccgc
     7561 ttcgtttatt gcctacgctc ccgactacat caaaaagata aatgaatttt caagtgatat
     7621 tagtaccgct tcgttggact tgggaacaaa aatcatgctc ccaaactctg acagcaaggg
     7681 caaggacagc gtggacttga taagggacag cctattttct attcaagtgg aacagccgtg
     7741 gttactctta caattcggga acagcaacgc agaagaaatc ggggcagacc gtgtagaagc
     7801 tctggtatcg gcaagcccag aggacgagga cggaaagacc agagaggaag tcgtgaaaac
     7861 agaaatcgag gacaacgaca acaacaatct gacgataccg caggtcgtga accgtttagg
     7921 tatggtgttc ttcctactgt tcttcaactt agggataacg atatttgtat tcttgcttac
     7981 gggcatgatg ttattcagcc agatactttt tattatcttt gctatgtttc tgcctatcag
     8041 ctttttactt tccatgatac cgagctatga aagcatggca aagcaggcaa tcgtgagggt
     8101 gtttaatacc ataatgacac gagcaggaat aacgctcatt gtaacggtgg cgttcagcat
     8161 ttccagtatg ttttataaca tatccacgga ctatccgttt ttcatggtgg cgttcttaca
     8221 gatagtgtgt ttcgctggta tctacatgaa gctgggtgac ttaatgagta tgttctcttt
     8281 gaacgctaat gatagtcaaa gcatgggacg aagaattttc cgcagaccat atctatactt
     8341 ggcacatagg gctagacgta tggaaagacg gcttgctggt gcggttactg ctggtggtgt
     8401 tgctggtgcg gtggctggta gcacgatttc aaacagaaaa gcgacaagga acacagctac
     8461 aaaagatagt cggggtaata catcttccag catgggacag cgtgtaggtt caaaggtggg
     8521 tgctgtctta gatacaaaaa ataaagtgaa agataaggca agtgctgtca aagagaatat
     8581 caaggatatg ccgacacaga ccgcttatgc cgtccactcc gcaaaggaaa aggcaaagtc
     8641 cagcgtgtcc gacttcaagc gtggcatggt gcaggaacag cagtccagac agtcgggacg
     8701 tatggaaaaa cgggaacagc ataggcagaa tatcgccgat aagcgtatgg agcttcaaaa
     8761 ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa cgggagctac
     8821 ccgtccccat gagcgaccag ccacagcttc aaccattcca aagccgagtg ctgaaaaaac
     8881 gcaggaagtc aaacgccctg ccacagcgac gacttcaaaa acaaatgagc cagtcaagac
     8941 aacggttatc aaagagcgtc ccttatcttc gggtacttct gataggaaaa cgagccagcc
     9001 tacacagcca gtacataggc agaatgtaga aaaagtggta tcgcaggaaa cacgccagaa
     9061 tgacaccaaa gaccgcagga caaaagttca gcagacgcaa agcgtccaga aaaaccagca
     9121 gaccacagag aaaacccgta accttgtaat gaagaaagga cagaagaaaa aatgaaactg
     9181 aaacatattg ctattatcgg cagtctgttt cctatcctct tttcactggt gcttttcttt
     9241 ggggtgttga ttagtgcgga cagcgacgac gagaacagca acttttcttc tggcattacg
     9301 ggtatgaact tatccgcaga agtcttgaaa catcagccta tggtggaaaa gtacgccaga
     9361 gaatacggca tttccgagta tgtcaatgtg ttgctggcaa tcatacaagt ggaaagtggc
     9421 ggtacagccg aggacgttat gcaaagctct gaaagtctgg gactaccgcc caattcatta
     9481 gatacagaaa gttcaatcaa gcaggggtgt aaatattttg cgtccctgct ttcgtcctgc
     9541 aaaaatcaag gtatggacga tttgaatgta gcgatacagt cttataacta tggcggtggt
     9601 tatgtgggat atgtggcagg aaaaggaaat aaacacactt tcaaccttgc agagagcttt
     9661 gcccgtgaaa aatcgggtgg aaagaaagta acatacacca atccaatagc tgttgcgaag
     9721 aatggaggct ggcggtatag ctatggaaat atgttctatg tggaattagt caatcagtat
     9781 ttaactgttc cgcaggtgtc gggagagctg gcacaaaagg taatgaatga agcgttgaag
     9841 tatcaaggct ggaagtacgt ctatggtggt agtaatccca acacttcctt tgactgttcg
     9901 ggactgacac aatggtgtta tggaaaagct ggtatctcct taccgagaac ggcacaagca
     9961 cagtatgatg ctacccaaca tctcccactc tcgcaggcaa aggctgggga cttggtattt
    10021 ttccattcta cctataacgc tggttcgtat gtaacccacg tcggcattta tgtaggaaat
    10081 aaccagatgt accatgcagg cgacccgata ggatttgcag acctaagcag tagttactgg
    10141 caacagcact taatcggtgc aggacgagta aaacaataga aaggacttga agatatgttt
    10201 aagaaaaata agaaacagac agaaactatc aaagaaccaa aagaaaaaaa ggtgcgtact
    10261 gtcaaggtag gcacacataa gaaaactgtg attgccttgt gggtggtgct tatcgcaagc
    10321 gtgagcttcg gggtgtataa gaattttacc gctatcgacc agcacaccac ccatgaaaaa
    10381 gaaatcattg agcttcgctt gcaggacacc aacgggatag aaaatttcgt gaagaacttt
    10441 gcgaagtcat attacacatg gaataacagc aaagaagcga ttgaagcaag gacgcaggca
    10501 atcagcggtt atctgacaaa ggaattgcag gacttgaata tagataccat tagaaccgac
    10561 ataccgacca gctccacagt tacagatgtg attgtatggc atatcgagca atcgggaacg
    10621 gatacttttt ctgctaccta cgaagtagat cagcagataa aagagggcga acagacaagc
    10681 aatgtgaaag caacctatac tgtaaaagtt catgtagacg ctgacgggga tatggtaatc
    10741 gttcagaacc ctactcttgc accagcaatc gaaaaatcag actatgatcc aaagacacca
    10801 gaaacagacg caagtgtaga cgctgatact gcaaatgacg ctaccgcatt tctggaaaca
    10861 ttctttaagt tgtacccaac agccacagaa aacgaacttg cttattatgt gtcggggaat
    10921 gtgcttgaac ctatcggcag ggactacctt tattctgaac tggtaaaccc tatctttaca
    10981 aaagacgggg agaatgtaaa agttaaggtt gctgtaaaat tccttgataa tcagacaaag
    11041 gcaacgcaag tatcgcagta tgagcttgtg ctacataagg atagtaactg gaagattgta
    11101 ggataattgt aaaaggcttg tatcatattt ttatgctctg caagcctttt aatattgaca
    11161 actgtatttt taagtattat gctgtacttg tataagctat acagtataga acaaggcggt
    11221 gtttaaatgg aaatctttat caataatcat gcaagcatac ctatttatga acaaatttct
    11281 tcgcaaataa aagcattgat tataaatggg gaattaaagg ctggcgaagc tctaccatca
    11341 ataagaggac ttgcaaaatc attgcatata agtgtattga ctgttcaaaa atcttatgat
    11401 gtgcttcaaa aagacggttt tatcgaaaca acagcaggca aagggtgttt tgtatctgct
    11461 caaaatcaag atttttatat agaagaacaa cagaaaaaaa tcgaagatag atttgtagaa
    11521 gctattgaaa tagcccgtgc aagtggcata aaccttgata aattgattgg attattaaaa
    11581 ttattatatg aggaggacga gtaaatggct gaaaatattt tagaagttaa aaaccttaca
    11641 aaagattatg gcgattttgt attaaatgac ttaagttttt ccattcctag aggtgtaatt
    11701 atggggctta ttggcgaaaa tggagctgga aaatcgacca cagtaaattg tattttgaac
    11761 gaaattaaga aaaacagtgg aaacattttg atttttggaa aagaccacat aattgatgaa
    11821 tgtgaaataa aagataaaat cggaattgta tttgacgaaa accactttcc agatattttt
    11881 acaccaaaag aaattgaaaa atatatgtcg ggaatatata ccaattggga cagtatagca
    11941 tttcgtaatt atttgaaaca gttcgaatta ccagcaaaga agaaaattaa agatttttct
    12001 aaaggaatga aagtaaaact tgcattcgct gttgctcttt cacataaagc agaattactt
    12061 atattagatg aagcaacaag tggattggac cctattattc gagatgatat tcttgatata
    12121 ctgattgatt ttgtgcaaga tgaaaattac tctattttgt tttcatcaca tattacaagt
    12181 gatttagaaa aaatagcaga ttatattact tttattcata aaggacggtt agttttctgc
    12241 tcattaaaag acgacttaat tgataactat ggtgttgtaa gttgtggagc aagtgtcttt
    12301 gatacgctag ataagacaga aattatttcc tatcaaaaag aagattatca atataaggtt
    12361 cttgttcgtg atagaaacaa gattgcaaaa gattttccaa aagcacttgt agaacctgct
    12421 actattgaaa atattatgtt gttttacgta aagggggaat tacaatgaaa ggcttattga
    12481 tgaaagattt attagtaatt cagaaaaaat atggaattac tagaattatt atggatatat
    12541 tcattatagt agctttgatg ttatttttaa aagggacagg agctatttac attagtttat
    12601 tgttaattcc agttgaaata atgacaatga taatttcctt aacgacttgc gacgaacaat
    12661 ggaaatgggg gaaatatgtt gttgcactac ccgtttcaaa aactacgatt gtaaaaagca
    12721 gatatatagt tgctttgatt atgacttgta ttggctttgg ggtagctttg cttgttaata
    12781 ccataacata ttttatattc cctcaatttg catttggtta ttatatgttt attgcttgtg
    12841 cttcttttgc cgtaacctta gtatttttgt catttgtatt gccttcaaat tattcacttg
    12901 gagttaatgc aggttttgcg gttatgttta ctctagtgat tattcttgtt gtattggcag
    12961 tttggacaaa tgtgactaac aattctattt tatggtttgt agtcgataat tttgaattga
    13021 gtatcggcat agcttttata atagttatag ctcttgcttt cagttcttat cttctatcaa
    13081 aagcattttt tactagaaaa tacaattagt gaggtattgt tatgagaaaa tgtcttagat
    13141 gtcatgaaga aatggtcgaa aatttagatg ttaaagttga tatgcaggga tatggtatta
    13201 gaataactac taaaggcgtt tttggaacta cggtagaaaa acctaaagtt gcagtttgtc
    13261 caaaatgtgg cgaagtttcc ttatatattg aaaattttaa atcaataaaa taaatgatta
    13321 aaatctgcat atagactgag ggtttattgt ttgctaacgt ccgagcggat attgaagaat
    13381 ttatgaatag tacataccca gagatgcaag caaatttcaa aaggtatttt ggaaatagac
    13441 attttacgcc gaaaaaatat gtttatatca ctacgaaaaa gcaaaaatta aatttcttaa
    13501 aagaataagg gggggctatg gataagcaat tatgggtatt atgtcctatt tgcaataata
    13561 agacacgaac taaagtgcga gaagatacag aactgataaa ttttcctttg ttctgtccga
    13621 agtgcaaaac ggaaacttta atcaatataa agcatggaaa agtaactatt atcaaagagc
    13681 cagacgcaaa gacgcagagc cgataatttg taggttatta tgacttatag attgttggct
    13741 cttatttgtt acctgcggag cagatcagca aatagataat gcagaatagc aaaaagggaa
    13801 aacaagaagc tgattacttc ttgctctccc tgctgttcaa gataccgtca atcgtacctt
    13861 gaatgatttt taattcgtta tcactcaata aatcaagaga ggtttctatc tgacggcgag
    13921 ctgtactttt tacgacgttc tctgctggat agaaatattc atcgaccgat atattgaaca
    13981 tggtaactaa atcatggaat aattgaaaac tagggtgtga accaacattt tcaatatctg
    14041 cgatatggcg ttctccatag aacactttat cgcctaaatc attacgagaa agccctgctc
    14101 tctcacgggc ttctctgatt gccagtccta aaggtctgaa atcaaaagtt tgtgtttctt
    14161 ttgcttttct cattttaaat cacctagcat gagtttatac ttcatgctgt atatttagaa
    14221 tgtgttaaaa ttgagcgaat cattcaatta gaaaacgaaa aagaatagaa tcacaaatca
    14281 cttgtgattt gtgattgttg atgataaaat aagagtaaga agaaatagaa agaagtgagt
    14341 ggttgtggga aatttaggcg cacaaaaagc aaaacgaaat gatacaccaa tcagtgcaaa
    14401 aaaagatatt atgggggata agacggttcg tgttcgtgct gacttgcacc atattataaa
    14461 aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt atggataaag ccttagaaga
    14521 atatattcgg aaatatttac ctgacaaact ttaaaaggag tgaaaatgaa atggcagtta
    14581 cttatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc gtttataatc
    14641 gagtattaaa ctatgttttg aatcatgaat taaataaaaa tgactctcaa ttattggaag
    14701 tcaatttatt aaaccaatta aagcttgcaa aacgtgtaaa tctttttgat tattctttag
    14761 aagaattaca agccgttcat gagtattggc ggtcaatgaa tcgctattca aaacaagttt
    14821 tgaataaaga gaaagtggct taatatggca aatatagtca attttactga caaacaattt
    14881 gaaaatcgct taaatgataa tttagaagaa ttggttcaag gaaaaaaagc ggttgaatcg
    14941 ccaaccgctt ttttacttgg tgggcaacca gggtcaggga aaacgagttt gcgaagaaga
    15001 tgattgagca ggcaggaaca aagtgataaa ttcaaattgt gagggaaaat atagatgaat
    15061 caaggaagaa ttattgtgat tacaggctca ccggggacag gaaaaacaac aactgcgtca
    15121 attgtcgcaa aggaatcaga tatggataaa tcagtgcata tgcacaccga tgactttttt
    15181 cattacttaa gcaaaggcgc aataccacca catttgccag aatccaacaa gcaaaatctg
    15241 gttgtcattg aagccttttt agaggctgca aagcgatatg ctcgtggcgg atatgatgta
    15301 attgtagatg ggattgtcgg gccatggttt ttggagccat ggaaagctct tgcccaagaa
    15361 gattacgagg tacactatat tgtattaaga gcgagtatgg aaataagact tagaagcaaa
    15421 cttaagagtg tgttgatagt gcagtatctt aaaattttgt ataataggaa ttgaagttaa
    15481 attagatgct aaaaatttgt aattaagaag gagtgattac atgaacaaaa atataaaata
    15541 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
    15601 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
    15661 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
    15721 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
    15781 tcaattccct aacaaacaga ggtataaaat tgttgggagt attccttacc atttaagcac
    15841 acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc tgattgttga
    15901 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
    15961 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
    16021 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
    16081 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
    16141 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccgttac
    16201 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
    16261 ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat gtagataaat
    16321 tattaggtat actactgaca gcttccaagg agctaaagag gtccctagcg cctacgggga
    16381 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
    16441 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
    16501 ctgaatgtac cttcacttta gctgaacatt ttagaaagaa aaaactcacc aaaacaaatg
    16561 taaaagaatt actagaggga aaagaaaccc tggtaaaagg aatcaaaacg aaagatagaa
    16621 agtcctacaa tgccgttgta aaaatcggag aaaagggata tattgatttt atctcttttt
    16681 caaaataagc ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat
    16741 cacaaatcac aagtgattaa tcacaaatca cttgtgattt gtgattccta atgatacaat
    16801 attactatac aaaaaaagaa tggggcgtag ttatggagaa ggaagaactc aaaatacttg
    16861 aagaattaag acgtatttta aacagtaaaa atgaagcgat tgttatctta aacaattatt
    16921 ttaaaggtgg tgttggaaag tcaaaattat cgactatgtt tgcttacttg acagacaaat
    16981 ttaatttaaa agttttaatg atcgataagg acttacaagc aacattgaca aaagacttag
    17041 caaaaacatt taaggtagaa ttgccacgtg ttaattttta tgaaggactg aaaaatggaa
    17101 acttggcttc ttctattgtt catttgactg ataatttaga cttgatccct ggcacgtttg
    17161 atttgatgtt actgccaaaa ttaactcgct catggacttt tgaaaatgaa agtagattgc
    17221 ttgctactct tttagcacct ttaaaaagtg actatgatct cattattatt gatactgtac
    17281 caacgccaag cgtttataca aataatgcaa tcgtggcaag tgattacgtc atgatccctt
    17341 tacaagcaga agaagaaagt acaaacaaca ttcaaaacta tatttcctat ttgattgatt
    17401 tacaagaaca gtttaaccct ggactagata tgatcggttt tgttccttat ttagttgata
    17461 cggacagcac aacgataaaa tcaaacctgg aagaactgta caagcaacat aaagaagata
    17521 acttggtttt ccaaaatatt atcaagcgaa gtaataaagt aagtacctgg tctaaaaacg
    17581 gcattacaga acacaaaggc tatgacaaaa aagttttatc tatgtatgag aacgtatttt
    17641 ttgaaatgct tgcatatagt atgcagtaat aattcaaaag ctgttatcgt gtgcggtaaa
    17701 taaaaaaaac cgttcctaga taatacactt ttgtgcgtct tgaaaaataa cgggacggtt
    17761 ttggatataa ccagaactgt ccttttttct taggagtaaa taaggcatag ctctggttaa
    17821 agggggctat accatgattg catacggaag aacgttctgc cgttctcaac tgaataaata
    17881 tttcagtcca ctattacaat cattacaccc tttacaagaa ttaaaaattc atatcaatag
    17941 aatttataaa acagtctgtt tgtgtaatta cacattatcg ggctgttttt gttttgttaa
    18001 aaattttttt caactttttt cggtttcaac ttagcaaatc acacctgtcg ttccctatat
    18061 ggtgcggaaa gagggataac cacttttcat atcgtaacgg aaagggggtg agattgatga
    18121 aaccatctga cttccagaaa acagttcaat gccgttttga aagttgtttg aagaaagttg
    18181 tccgacatgt tgttaaggac tatcagaaga aattaaagcg acgacaaaag ggagaaacgc
    18241 ttttttgtga acttccagaa atcgttgtag aaaatctggc tgtctgggac gactatgata
    18301 cggactatac gattttcaat gtatgtggta gtgatattcg tatttgtgat gatgaactgg
    18361 cagaagcctt gaaacatttg tctgagcgta atcgagaaaa tttgttgatg tattatttct
    18421 tggaaatgag cgataccgag attgcaaaaa gacaaaatat ttcaagaagt ggtgtttttc
    18481 aaaaccgaca caattcacta gagcttatga aaaaaatact aaaggagaaa tgatagcaaa
    18541 tgaaacacac aatgaagaag ccgtcctact acttgctttc gcaggcaatg gacggggacg
    18601 aaaaagcgat tgaaaagata ctggcatttt atgaccctta catatcaaag tgctgtctac
    18661 gaccactcta tgatgaatac ggcaatgtct atattgttgt agatatggaa ttaaagggac
    18721 ttatcagaga agcacttatt aaaatgattt tagggtttga tattgcctta gaaatcgaag
    18781 aagaataaca agcagtaacc gcagagcatg atttgttcaa tcccctcttt cctgctctgc
    18841 ccctgctttt atatgaaagc aacacgcttt cgccacagct ctttgacaac tgaataaagc
    18901 agacagatac gtttgtgaga tagcgagcca cgggaactgt acgccacgac cttttcaaaa
    18961 gaaagcgagc gacccacgca gtgatccgag agcgactgtt ggaaaagtcg ttttgccatg
    19021 acctatcctc acagaataat gatacgtccg catggtgcgg ttctgcccac aagaatggga
    19081 atagttgaga tactaacgga gctttccaaa gccgtctgcc tgcttataat tgatagtttc
    19141 agcaacaaaa tctatctact ctttttattt aggtgtgtta taatgacctt attagacgag
    19201 tgtattttgt tgctgtcaca aatgaaaggg gtcttaatag tgataagtga tacacaaaat
    19261 gcacaatctc ctacaaagga aaaaaagact tatacagtag aggaaattgc tgaacaactg
    19321 gacattagca aaaaggtagc ttattcatta gtaaaaagcg gtcaattcag ttatgttcgt
    19381 gctggtaaag ccatacgggt atcaaaggtt tctttcgata aatggttaaa tgaaatttaa
    19441 ctataaggag agaaaattat ggcttcaatc aaacaaagaa aatcaagttt ttcagttatt
    19501 tattggtact tagatagtgc aggagaaaga aaacaaaaat gggacacgct ggaaacaaga
    19561 aaagaagcaa aacagcgaaa agcctttata gaatactatc aagagaaatt tggatatgtg
    19621 atagttccat tagaggaaca atttgcacat cagattgaaa attctaaaaa ggagttagat
    19681 gttgtagaca aagatattac attgcgtgat ttcctagaaa tttttgttaa tttatatggc
    19741 acatctaaat ggtcgccgag tacatttagt agtaaggtag gcagtattga aaattatatc
    19801 aatccgatta ttggagattg gaaactgaat gaaataacta caaaaagtct atcaaaatac
    19861 tacaatgatt tattgagcgt tccagaagta ccacgggcaa acagaaaagc aactggtcgt
    19921 tgtgttcaac cagcgaacat caaaaaaata catgatatta tccgctgtgc cttaaatcaa
    19981 gctatccgct gggagtatat tgatacaaac aaaagaaacc ctgcgtcttt ggctacgtta
    20041 ccaaaaattc caaagactag gcgaaaagtg tggagtgttc aaacatttcg agaagcggta
    20101 aaagccacgg aagatgattt gctttcaatt tgtatgcact tagctttttc ctgctctatg
    20161 cgaattggag aaattacagg acttacatgg gaagatgtta ttattgatga agagtccatt
    20221 gctacaaata atgcaaaggt aatcatcaat aaagaacttt caagagtaaa tgcagaagct
    20281 atgcagaaat taaaggaaaa ggatatttta aagattttcc caacgcaaaa accgcattgt
    20341 accacaaggc ttgtcttgaa aacgccaaaa acagaaacaa gtaatcgtgt tgtctggtta
    20401 cctaaaacag ttgcggaact attggtgcaa tataaaaaag accagcagga attaaaggaa
    20461 tttttaggaa gtgcctacaa tgattacaac cttgttattg cgttggataa tggcaatccc
    20521 gtggaaagcc gtattgttag agataggttt caaaagctct gtgaagaaaa cgattatgaa
    20581 gttgtggtat ttcacagcct acgccattta agtacgggtt ataaactcaa aatgacaaac
    20641 ggagatgtta agtccgtaca aggcgatact ggtcacgcag aagcagaaat ggttacagat
    20701 gtatattcag aaatcattga cgaggacaga cgtttcaatg cacaaaaaat ggataaagaa
    20761 ttttattcta cgcttaatga tgatacagac aatccaaagc aagataccga cttaaccgat
    20821 agtgatatgg cgttattgga actgattaaa tctttatcgc cagaaatgaa agagcaattt
    20881 ttaaagcagg cacttcaaag atagaatttc aaaagtgtta gcaaattcca aaaaaaccag
    20941 cgatttttct tcaaaaataa aaaagtgtta gcaagcctat ttccttaaaa gtcttaaaaa
    21001 aatatcgaat aaaaaatgac agattgaaaa caattcgtat tcagcgtaaa tagaaagcca
    21061 taaataaaga aacgctgaaa accttgtaaa ataaggcttt cagcggagtt tgtggcgtcc
    21121 cagacaggag tcgaacctgc gacgtttcgc ttaggaggcg aaccctctat cctgctgagg
    21181 tactgggaca ttcttaaaaa tctattcaat tttatctagt tatcatatca aaaggcgttt
    21241 gttccaccta gcccttagga ggtctccatt cagcagcgaa caagttacct ttaccattag
    21301 gagtagaaaa cttttgacct tcatacagat acatagttcc tttatcttcc atactttcac
    21361 tcttacaagg ccattgaaca cacccttgag tttctatctt ttcataagta gcacctaaga
    21421 aacttggaca taattgtcta agttcatccc atatttcctt agtattttta taattcatag
    21481 gatatcccat tgctgtagaa atttcgctta taatttgcca gtctggtttt acatcccctt
    21541 gtggctctat agcttttctc atcaattgga atcctctatc tgcacatgta tatactccct
    21601 catgttctcc ccaagaagta gctggaagta taacatcagc gtgaagtgct gtcttattca
    21661 taaatatatc ttgaacaatg acaaattcca attcatctaa tgcttctcta acttctgatg
    21721 catctgggtc actttgcact gggtcttctc caaatatata ataagcttta agctttttct
    21781 cttttagtac taagtctgga acttgagtta aactataacc attattagga gatagtttta
    21841 ctccccaagc tttttcaaac ttttctctta tcttatcatc agttacattt tgatagccag
    21901 gatatacatt aggaagagct cccatatcac aagctccttg aacattattt tgtccacgta
    21961 caggtcctat tccaacgctt tctctaccaa aattacctgt aagtagtgca attgatgcaa
    22021 gtcccttaac tacatcaaca gcttgaccaa attgacagac acccattcca taaagtatca
    22081 tagctttttt accttttgca tattctctca tagcttttct tataagctct tctgggattc
    22141 ctgtaatctt ttcagcatat ttagctgtat atggtttaac aatttcctta tactcatcaa
    22201 atccttgtgt atgattttga acaaattctt tattgtaaag accttcttct attaatacat
    22261 ttccaaaagc atttactaaa accatatttg ttccaccttt tataggtaga tgtatatcag
    22321 caattcttgc agattcagtt actctagggt cagtaacaat taatttagca ccattttttt
    22381 tagcttttac tattctattt gccactattg gatgtgaatc tgctccatta tatccaaata
    22441 taaataaaac atctgcattt tctatctctg gtatagaatt agacattgca ccactaccta
    22501 atgagtaagc cagaccggct acagatggtg catgtcagac acgagcacaa tggtcaacat
    22561 tattagttcc tattgtagct ctcataaatt tttgcattat atagtttgct tcattaccag
    22621 gtcctcttgc agaacctgtt cccataatag aatcaggtcc atatttttct ttaatttcat
    22681 ttagtcttga agctgtatag tttatagcct catcccattc aacctcttct agtacacctt
    22741 gttttcttat cattggcttt tttagcctag gtgttagaat tttagggtca tttagaaaat
    22801 cccatccata acgacctttt aaacacaaac tttcttgatt tgttctacca ttagccggtt
    22861 cagctcttaa tatcttatta ttttttacta ctagatataa ttggcatcca gctccacagt
    22921 atggacaaac tgttaaaatt tttttctcca tacaattccc cccttagatt tatcccctga
    22981 taagtaaaat ttatcttaaa gataatgagt gtttttataa tatgcaaaat cataaatatt
    23041 ttgcaatact atctattaaa ttcaacaatt ttctcacaat tcctcttaaa ttttaaaaaa
    23101 aatatgtcat aataatcttt atatttcaac catttaatta aaatattcta gttcttatga
    23161 catatttttt aatatactca atatatattc tttaaaccta tttagttttc acttaaaaca
    23221 atatttctgc caattctatt tttcattcta tagtgagtaa ttacagtaaa tattccactt
    23281 ccaatcagaa tcaaatagta cataaatcca ccatataaaa atactgcata tcccatcaaa
    23341 ggttgaggga atactgattt gaatatagtg aaaaatgcta attcattagc tcctacattc
    23401 ccaggtgttg gtattggaga tattgccata tataaaaatg cttgtaatgt cagtatataa
    23461 atatagctat aaccttgtaa attaaatgct ttatatatcc aaaatgaaac actaaagtaa
    23521 gcacttaatt gaataaaagt tactattata gttgaaatta ataccttttt attctttgca
    23581 aaaaaattta ttgctttact ataatcatct ataaaacttt ctatggattc aatcttatta
    23641 tccaaaaatt ttaaaaactt aatttttgat aaatgcttta tagtaattct cataaaatat
    23701 tttatttttt tagggtttaa aataactaat attatcataa ttaagacaaa tgaattaatg
    23761 gctatcccta gaaacactag aggcataatc attttcattt gtttttctaa catagcaaaa
    23821 ttcattaata caagtattgt acaataaaaa gtaactacta tttggaaaat aatagactta
    23881 ttggtaacaa ctgcacttgc cttactaagt ggcattttac attttttaag tacatatatc
    23941 tgtactggtt ggcttcctga agcaaaagga gttattaaat tataataaaa tcccataatg
    24001 gctagcttaa aaccaataaa tctaatatta actttgtgag tgctttctat aattatcttc
    24061 attactactc cttcaagcat tatgtgacac attattgcca aagcacctat gaataaaaac
    24121 ttcttatcta ccataaccat aacatttgat agcattttaa tatccaatgt tttaaacact
    24181 aggtaaattg ttatacctat taacaaaaat aaaaatgtat actgtagtaa actctttcta
    24241 atattcttta gtctatccat tagaaataac catcctttgc tcaagattta tactaaatac
    24301 tatattatta gtatacacta tgaatcttaa tattttataa caaaatacct tacaattttg
    24361 tcatttacaa aaaatattaa tctataaatt aaattttaat atatagatta atattttaca
    24421 aacattttgt aaatatatat
//
LOCUS       DAEMAE010000473        16228 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.078, whole genome shotgun sequence.
ACCESSION   DAEMAE010000473 DAEMAE010000000
VERSION     DAEMAE010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.078"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..138
                     /locus_tag="KPH93_003864"
                     /pseudo
     CDS             1..138
                     /locus_tag="KPH93_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            161..1366
                     /locus_tag="KPH93_003865"
     CDS             161..1366
                     /locus_tag="KPH93_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG1690560.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            1409..1630
                     /locus_tag="KPH93_003866"
     CDS             1409..1630
                     /locus_tag="KPH93_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1690561.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            1747..2244
                     /locus_tag="KPH93_003867"
     CDS             1747..2244
                     /locus_tag="KPH93_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1690562.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            2219..2725
                     /locus_tag="KPH93_003868"
     CDS             2219..2725
                     /locus_tag="KPH93_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1690563.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            2709..5156
                     /locus_tag="KPH93_003869"
     CDS             2709..5156
                     /locus_tag="KPH93_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1690564.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            5159..7336
                     /locus_tag="KPH93_003870"
     CDS             5159..7336
                     /locus_tag="KPH93_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1690565.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            7333..8334
                     /locus_tag="KPH93_003871"
     CDS             7333..8334
                     /locus_tag="KPH93_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG1690566.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            8331..9266
                     /locus_tag="KPH93_003872"
     CDS             8331..9266
                     /locus_tag="KPH93_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1690567.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            9509..9625
                     /locus_tag="KPH93_003873"
     CDS             9509..9625
                     /locus_tag="KPH93_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBG1690568.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9641..11560
                     /gene="tet(M)"
                     /locus_tag="KPH93_003874"
     CDS             9641..11560
                     /gene="tet(M)"
                     /locus_tag="KPH93_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBG1690569.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            11679..11846
                     /locus_tag="KPH93_003875"
     CDS             11679..11846
                     /locus_tag="KPH93_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG1690570.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(11906..12259)
                     /locus_tag="KPH93_003876"
     CDS             complement(11906..12259)
                     /locus_tag="KPH93_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1690571.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            12764..13186
                     /locus_tag="KPH93_003877"
     CDS             12764..13186
                     /locus_tag="KPH93_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1690572.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            13183..13413
                     /locus_tag="KPH93_003878"
     CDS             13183..13413
                     /locus_tag="KPH93_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1690573.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            13874..14077
                     /locus_tag="KPH93_003879"
     CDS             13874..14077
                     /locus_tag="KPH93_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG1690574.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            14159..15376
                     /locus_tag="KPH93_003880"
     CDS             14159..15376
                     /locus_tag="KPH93_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1690575.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            15601..16026
                     /locus_tag="KPH93_003881"
                     /pseudo
     CDS             15601..16026
                     /locus_tag="KPH93_003881"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
     gene            16069..16228
                     /locus_tag="KPH93_003882"
                     /pseudo
     CDS             16069..16228
                     /locus_tag="KPH93_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
BASE COUNT         5360 a         2878 c         3345 g         4645 t
ORIGIN      
        1 taagccacgc cagcaggtaa aacccctcgg tatctaacag gggggtacaa atcgacagga
       61 aacagtcaaa aaaacattag aaaatccttt ggttacaagg gatttacaaa atttcagcgt
      121 atgtcaaatg ggctttaaaa gttgacatac gcctttttga ttggagggat ttttactgaa
      181 tgaacaaact tggttacagc atttaaaaga aaaacgcttg gcttatggac tatctcaaaa
      241 ccgtttagct gttgcgactg gtattacaag gcagtatcta agcgatattg aaacaggaaa
      301 agtcaagcca tcagaggatt tacagcagtc cctttgggaa gctctggaac gcttcaatcc
      361 cgacgctccc cttgaaatgc tgtttgatta tgtaaggatt cgctttccga caacagacgt
      421 acagcaggtg gtcgaaaaca tcttacaact gaaactgtcc tattttcttc atgaggacta
      481 tggtttctat tcttattcag agcattatgc tttaggcgac atattcgtcc tttgctccca
      541 tgaactggac aaaggagttc tggtggaatt gaaaggtcgt gggtgcagac aatttgaaag
      601 ctatcttctg gcacaacaaa gaagctggta tgagttcttt atggacgttt tggtggctgg
      661 cggtgtgatg aaacgccttg accttgccat taacgataag acagggattt tgaatatccc
      721 tgtactcact gaaaagtgcc aacaggaaga atgtatctcc gtcttccgca gttttaaaag
      781 ctatcgcagt ggcgaactgg tacgcaaaga ggaaaaggaa tgtatgggaa acaccctcta
      841 tatcggttca ttacaaagtg aagtttattt ctgtatctat gaaaaggact acgagcagta
      901 caagaaaaat gatattccca ttgaagacgc agaagtaaaa aaccgttttg agattcgatt
      961 gaaaaatgag cgtgcctatt atgcagtccg tgatttactc gtctatgaca atccagagca
     1021 taccgccttt aaaattatca atcggtatat ccgttttgta gataaagacg attccaaacc
     1081 tcgttctgat tggaaactga atgaagaatg ggcttggttt attgggaaca atcgtgaacg
     1141 attaaaacta accacaaaac cagagcctta ctccttccaa aggacgctga actggctatc
     1201 tcatcaagtt gccccgacct taaaggttgc gattaaactt gatgaaatca accagacgca
     1261 ggttgtaaaa gacattctcg accatgcgaa actgacagac cgacacaagc agattttgaa
     1321 gcaacagtca gtaaaagaac aggacgtgat aacaacaaaa aaataactca aatacaaatt
     1381 cattgaatat agagaggaga acatttttat gaattttgga caaaaccttt ataactggtt
     1441 tctatcaaac gctcaatcac tggtgctttt agcaatcgtt gtgattggct tgtatcttgg
     1501 cttcaagcgt gagtttagca aactgattgg ctttttaatt attgcgatta ttgcggttgg
     1561 cttagtcttc aacgctgctg gagtaaaaga cattttacta gagctattca atcgcattat
     1621 tggtgcttaa ataaaaccgt tcttttgtgg aatataagtg gttttcttat gttccgcaaa
     1681 ggaatggtac accaaacgaa gtgcggtagg gatttttgaa tctctacaaa gaaaggacgt
     1741 gaatatatgg acgatatgca agtctatatt gcgaatttag gcaaatacaa tgagggcgaa
     1801 ttggtcggtg cgtggtttac ctttcccatt gactttgagg aagtcaaaga gaaaatcggc
     1861 ttgaatgatg aatatgagga atacgccatt catgactacg agttaccctt tacggttgac
     1921 gaatacactt ccattggcga actcaatcga ctatgggaaa tggtatcgga attacccgaa
     1981 gaattacaat cggagctatc tgctctgctc actcattttt caagcattga agaactaagc
     2041 gaacatcaag aggatattat cattcattcc gattgtgatg atatgtatga cgtggcacgc
     2101 tactacattg aagaaacggg tgctttaggc gaagtaccag ctagtcttca aaactatatt
     2161 gattatcaag cctatggtcg ggatttagac ctttcaggaa cgtttatctc aaccaatcat
     2221 gggatttttg aaatcgtcta ttaaatctgt cggtacatta ctactggcag attttctatt
     2281 ttacggggtg gctcaatcag ctacccctat tttttatgaa aggattgatt acatgaagaa
     2341 aatacgaagc tataccagta tctggtctgt ggaaaaggta ctgtattcta tcaatgattt
     2401 tagacttccg tttcccataa cctttacgca aatgacatgg tttgtcgtgt cactctttgc
     2461 agtgatgata cttggcaact tgccccctct ttccatgata gagggagcat ttctcaaata
     2521 ctttgggatt cctgtggctt tcacatggtt tatgtctaca aaaacttttg atggtaaaaa
     2581 gccttatgga tttttgaagt ctgtcattgc ttatgcactg cgaccaaagc tgacctatgc
     2641 aggaaaaaaa gtaacgcttg gcagaaacca gccacaagaa gccattacag cagttaggag
     2701 tgaattttat ggcatatcca attaaataca ttgaaaacaa tctcgtctgg aataaagacg
     2761 gggaatgtta tgcttactat gagcttgttc cttacaatta ctcatttcta agtccagaac
     2821 agaaaataca agtgcatgat tctttcagac agcttatcgc acaaaatcgt gatggcaaaa
     2881 ttcatgcttt acaaatcagt acagaatcca gcatacgttc tgcacaagag cgttccaaaa
     2941 atgaagtcac tggcaagctc aaagcggttg cctatgacaa aatcgaccaa cagacagacg
     3001 ctttaatatc catgattggc gaaaatcaag tgaactaccg tttctttatc ggctttaagt
     3061 tgcttctcaa cgatcaggag ttttctatga aaagtcttac cgttgaagca aaaaatgctt
     3121 tgtctgattt tgtctatgat gtgaaccata agctgatggg cgattttgtt agtatgagta
     3181 atgatgaaat cctgcgtttt cagaagatgg aaaagctctt agaaaataaa atctctcgtc
     3241 gtttcaaaat ccgcaggtta gataaggacg acttcggcta tctgattgaa cacctttacg
     3301 gacagacagg cactgcctat gaagagtatg agtaccatct atcaaagaaa aagctggata
     3361 atgaaacgct gattaaatac tatgacttga ttaagcctac tcgctgtttg gtggaagaaa
     3421 aacagcgata tttgaaaatc cagcaggaag atgaaaccgt ctatgtagct tactttacca
     3481 ttaacagcat tgtcggagaa ctggacttcc cgtcctctga aatcttctac taccagcaac
     3541 agcaatttac attcccgatt gatacgtcaa tgaatgtgga aattgtagcg aatcgtaaag
     3601 ccctatctac tgtccgcaat aaaaagaaag aactgaaaga cttggataac cacgcttggc
     3661 aaagtgataa tgaaaccagc tccaatgtgg cggaagctct ggaaagtgtg aatgagctgg
     3721 aaaccaattt agaccaaagc aaggaatcta tgtacaagct gtcttatgtg gtaagggtat
     3781 cagcaaatga tcttgacgaa ctcaaacgtc gttgtaatga agtgaaagat ttttatgacg
     3841 atttaagcgt aaaactggta cgaccatttg gggatatgct cggcttacat gaagaatttt
     3901 tacctgccag caagcgttat atgaatgatt atattcaata cgtgacctct gatttcctcg
     3961 ctggtttagg ttttggtgct actcaaatgc tgggggaaaa tgaggggatt tatgttggct
     4021 acagcttaga tactggacgc aatgtctatc tgaaacctgc tcttgccagt caaggggtta
     4081 agggttcagt aaccaatgcg ttagcgtcgg cttttgttgg ttcgctgggt ggtggtaaat
     4141 cctttgcgaa taaccttatc gtctattatg cggtgcttta tggggcacaa gcagtgattg
     4201 tagacccaaa agcagaacgt ggcagatgga aagaaacctt gccagagatt tcccatgaaa
     4261 tcaatatcgt cactctgact tctgatgaga aaaacaaagg cttacttgac ccttatgtga
     4321 ttatgaaaaa tcccaaagat tctgaatcac tggctattga tattctgaca ttccttacgg
     4381 ggatttcctc tcgtgatggg gaacgcttcc caatccttag aaaagccatt cgtgcagtaa
     4441 ccaatagtga agtacgaggg ttgatgaaag tgattgagga attacgggtt gagaatacgc
     4501 cactaagtac cagtatagcc gaccatatcg aaagttttac agactatgac tttgcacatt
     4561 tattattcag taatggttat gtggagcagt ctatcagctt agaaaaacaa ctgaacatta
     4621 tacaggttgc ggacttggta cttcccgaca aggaaacttc ctttgaggaa tataccacta
     4681 tggagctttt atccgttgct atgctgattg tcattagtac ctttgcttta gactttatcc
     4741 atacagaccg aagcattttc aagattgtag atttagacga agcatggagc tttttacagg
     4801 tagcacaagg aaaaacacta tctatgaagc tggttcgggc tggtcgtgct atgaacgctg
     4861 gggtatattt cgtgacccaa aatacagacg acctcttaga tgaaaaactg aaaaataacc
     4921 tcggcttaaa atttgcattt cgttccactg accttaacga gattaaaaag accttagcct
     4981 tttttggtgt agacccagag gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg
     5041 ggcaatgcct tatcagtgat ttatatggtc gtgtcggtgt gatacagttc caccctgtat
     5101 ttgaagaact gctccatgcc tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt
     5161 gaaaccatca atagtaaaca gaataaaatc aaactggacg ctgaaacgtc taggtaaagt
     5221 ggcaatgaca gtggctttca cacttgtgat tgccattttt cttttagcca tgctgggaac
     5281 ggtggttcaa gctgcgggct tggtagatga tacggtcaat gtggcaaatg aatacagccg
     5341 atacccactt gaaaactatc aactggattt ttatgtggat aatagctggg gctggcttcc
     5401 gtggaactgg tcggacggga ttggaaaaca ggtcatgtat ggactatatg ccattaccaa
     5461 ttttatttgg acaatcagtt tgtatgtttc caatgcgaca ggttacttag tacaggaagc
     5521 ctattcctta gacttcattt ccgctacagc agattccatt ggtaagaata tgcagacctt
     5581 agctggtgtg agtgcaaacg gattttcaac agagggtttc tatgttggat tcctcttact
     5641 cttgattttg gttcttgggg tttatgttgc ctatacggga ctgataaaga gagaaaccac
     5701 aaaggcaatt catgccatta tgaattttgt gctggtgttt atcctatcgg cttcctttat
     5761 tgcctacgct cccgactaca ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc
     5821 cagtttatca cttggcacga agattgtcat gccccattcc gatagtcaag gcaaggacag
     5881 cgtggactta atcagagata gcctgttttc catacaggtt cagcaaccgt ggctactgct
     5941 tcaatacaac agttcagaca ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc
     6001 caccagccca gattccaaca atggcgaaga cagagaaaaa attgttgcgg aagaaattga
     6061 agacagaagc aataccaatc taaccattac aaagaccatt aaccgtttag gtacagtctt
     6121 cttcctattt gtcttcaata ttgggatttc catatttgta ttcctattaa caggaatcat
     6181 gattttctcg caggtacttt ttatcatcta tgctatgttt ctgcctgtga gctttatttt
     6241 aagcatgatt ccatcatttg atggtatgtc aaaacgagcc ataacaaagc tctttaatac
     6301 cattttgaca cgagctggaa tcacattgat tattacgaca gcatttagta tttcaaccat
     6361 gctctatacc ttatcggctg gttatccgtt ctttttgatt gcttttctac agattgtgac
     6421 ctttgcagga atctacttca agctgggcga tttaatgagt atgttttctc tacagagtaa
     6481 cgattctcaa agtgtgggaa gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca
     6541 catgcaccgt ctacagcgga aacttggacg ttccatgact actctagggg ctgggtctgc
     6601 cattgttaca ggtaaaaaag gacagtcggg ttcggggagt tctgcaagga cacaagcaga
     6661 tcactcccga ccagacggaa aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat
     6721 cggtacagta gctgatacca aagacagaat ggtagacact gctagtggtt tgaaagaaca
     6781 ggttaaagat ttgccgacca atgcaagata tgcagtatat caaggaaaat ccaaagtaaa
     6841 agagaatgtc cgtgatttaa ccagtagtat ttctcaaacc aaagcggaca gagccagtgg
     6901 acgcaaggaa cagcaggaac aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga
     6961 acaggtcaaa cagaaaaaac agcctgcttc ttctgttcat gaaagaccga ctacaagaca
     7021 agaacaatat catgatgaac agacctcaaa acagtctaat attcagactt catataagga
     7081 atctcaacaa gccaaacaag agcgtccagc agttaagtcc gatttttcaa gtccaaaagt
     7141 ggaacgccaa ggcaataccg ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac
     7201 tacagcagat agaacttcac aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag
     7261 agtaccacta caaaatacaa gaagtagacc accaatcaaa accgccacca ttaagaaagt
     7321 cggtaagaaa ccatgaagtt gaaaacttta gtgattggtg gttctggatt attcttgatg
     7381 gtcttctcac tgcttctgtt tgttgccatt ttattttcag atgaacagga cagcggaatt
     7441 tccaatattc attatggagg tgtgaatgtt tccgcagaag tgctggctca taagcctatg
     7501 gtagaaaaat atgccaaaga atatggcgtt gaagaatatg tcaacatact tcttgcgatt
     7561 atacaggtgg aatcgggcgg tactgcggaa gatgttatgc agtcctcgga atccctcggt
     7621 cttccaccta attcattgag tacagaagaa tccattaagc aaggtgtgaa gtatttcagt
     7681 gaattattag ccagtagcga aaggctcagt gtagatttag aatcggttat ccagtcctac
     7741 aattatggtg gtggtttctt agggtatgtg gctaatcgtg gaaataaata tacctttgaa
     7801 ctggctcaaa gtttctcaaa agagtattca ggtggcgaaa aagtgtctta ccccaatccc
     7861 atagccatac ctatcaatgg gggctggcga tacaactatg gcaatatgtt ttatgtgcaa
     7921 ctggtaacgc agtatcttgt cacaacagag tttgatgatg atacggtaca agccatcatg
     7981 gacgaagcac tgaaatatga gggctggcga tacgtttacg gtggagcttc cccgactact
     8041 tcttttgatt gtagcggact gacacaatgg acgtatggaa aagctggaat taacttacca
     8101 cgaaccgcac aacagcaata tgatgtgacc cagcatatcc cactatcgga agcacaagct
     8161 ggcgatttgg ttttctttca ttctacctat aacgctggct cttatattac tcatgttggg
     8221 atataccttg gcaataaccg tatgtttcat gcaggcgacc caatcggtta tgccgactta
     8281 acaagcccct actggcaaca gcatttagtg ggagcaggac gaatcaaaca atgagaaagg
     8341 aagatttaat gatgaaattt agaaaaaatc agaataaaga aaaacagata ccaaaggaaa
     8401 agaaacctcg tgtctactat aaggtcaatc ctcataaaaa ggttgtgatt gccttgtggg
     8461 tacttttagg gcttagtttc agctttgcga tattcaagca ctttacagct atagatactc
     8521 atactattca cgaaacaact atcatagaaa aggaatacgt tgatactcat catgtagaaa
     8581 attttgtaga gaactttgcg aaagtctact attcatggga gcaatccgat aagtccattg
     8641 ataatcgaat ggaaagtcta aaaggctatc tgacagatga acttcaagct ctcaatgttg
     8701 atacagtacg caaagatatt cctgtatcgt cttctgtaag aggatttcag atatggacgg
     8761 tagagccaac tggcgacaat gagtttaatg taacctacag tgtagaccag ctcattacag
     8821 agggagaaaa tacaaagacc gtccactctg cttatatagt gagtgtctat gtagatggtt
     8881 ctggaaatat ggtactggtt aagaatccga ccattaccaa catacctaag aaatcaagtt
     8941 ataaaccaaa agccattgaa agtgagggga cggttgattc cattacaacc aatgaaatca
     9001 atgagttttt aacgacgttc ttcaagctct atcctacagc gacagccagt gaactttcct
     9061 actatgtgaa tgacgggata ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg
     9121 taaatcctat tcacaatcgt aaggataatc aagtcacggt atcgctgaca gtggagtata
     9181 tcgaccagca gaccaaagca acgcaggtat ctcaatttga tttggtactt gaaaagaacg
     9241 ggagtaattg gaagattata gaataacaaa tattggtaca ttattacagc tattttgtaa
     9301 tcacgtactc tctttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta
     9361 ttatttaagt atctatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa
     9421 ctgtcaattt gatagtggga acaaataatt ggatgtcctt ttttaggagg gcttagtttt
     9481 ttgtacccag tttaaatacc tttatcatgt gattctaaag tatccggaga atatctgtat
     9541 gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt atcactggga
     9601 tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta ttaatattgg
     9661 agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat tatataacag
     9721 tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct
     9781 tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa
     9841 tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg
     9901 ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc
     9961 acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat
    10021 caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa
    10081 actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac
    10141 gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga
    10201 gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag
    10261 atttcagaat tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat
    10321 tgataacctt atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc
    10381 tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata
    10441 tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga
    10501 aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag
    10561 agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct
    10621 tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca
    10681 aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga
    10741 aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat
    10801 actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta
    10861 tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa
    10921 tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt
    10981 atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg
    11041 atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg gttgcgaaca
    11101 aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg gtttatacta
    11161 tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat tggaacaagt
    11221 cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa tttatgcgcc
    11281 acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga acatcgtaga
    11341 cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc ggtgtattca
    11401 agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt taacagagtt
    11461 aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc caaatagtcg
    11521 gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat gttgttatat
    11581 aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg aattaaagtg
    11641 taaaggagga gatagttatt ataaactaca agtggatatt gtgtcctgta tgtggaaata
    11701 aaacacgatt aaagataagg gaagatactg aattaaaaaa attccccctc tattgtccga
    11761 aatgcagaca agaaaattta attgaaataa agcagttcaa agtaactgtg attacagagc
    11821 cagacgcaaa gacgcagagc cgataaaatg agattaatac aatctcattt tatcggctct
    11881 ttccgttatg tatggattct tttaattagt cttcgatgtt tcttgcttcg ttgataccgc
    11941 tggctaaaga ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttctcta
    12001 tctgtaatcg tcgggtgctt tttaccaagt tattagcagg taagaaaaat tcatcaacgg
    12061 aaacatgaag taacgataca aggtcataaa gaacttgtat gctggggtgt tgccctttat
    12121 tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa tgctcccact tgttcacgag
    12181 ttaaacctcg tttcaatcga gcttctttaa tggctaaacc aaaggctcta aaatcatatt
    12241 tatcttcttt tttacgcata gtagaccacc tctatacatt ttattgttcc tactgaatta
    12301 aaaacaggta tagaaaaacg tgttatatgg tttataggtt tatatttaat aaaaagcact
    12361 actaaacgcc aataaaaaaa accgttatat ggtagtgcta tttacgctgt taaaatattg
    12421 tatattactt ccaaatggcg gtttgttgga ggtcaacgtc gccatgaagt acatcatata
    12481 caataaattt ccttacattg ggttcttgtc aaaaaaagtc gtctatctgc aatagataag
    12541 tacgtccacc aatgtggttt tataaatcat atagatagaa taacagaagc atgtaaacag
    12601 agaaataaat ctgtttatat gcttttttgg ctattcagaa cttttttaca aagtttattt
    12661 atcagtaatg caacaaatcc ccctttcaca ttgggactaa gagtgaaagg agataaacga
    12721 gcaaggctca cttcctttcc tagacagaaa gggggtgaga aacatgaaac catcttcttt
    12781 tcagaccaca atagaaaatc agtttgacta tatctgtaaa cgtgctatgg aagacgagcg
    12841 aaagaattat atgctttatc tttcaaggat tgcaaagcgt gaggtgtcct tttcggatgt
    12901 tggcgattat cttgttagcc agtttgcgac aacagataac tattcaactg actttcagat
    12961 ttttacactc aatgggttat cagtaggcgt tgaaaatgat ttgttgagtg aagcattacg
    13021 tgagttgcca gacaagaaac gtgaaattct actgctgttt tactttatgg acatgagcga
    13081 ttcagaaatt gcagacctgt tgaaattgaa ccgttctact gtctatcggc atagaaccag
    13141 tggactagcc ttaattaaaa agtttatgga ggaatttgaa gaatgaaaac acaatatcct
    13201 atgattccct ttcctctcat tgtaaaggca acagatggcg ataccgaagc gattaaccag
    13261 attctacatc attacagagg gtacataacg aagcgttccc tacgacttat gaaagatgaa
    13321 tatggcaatc aaagtatggt cgttgatgaa gtcttacgtg gaagaatgga aaccagactg
    13381 attacaaaga ttttgtcatt tgaaattaag taatatcctc tctcctttcg tggaagcgtg
    13441 ctaaaccatt ccacgcttcc cgaacaggga ggtttgttat tccaccaaag catattgagc
    13501 tttcaatgtg ttttgatagg ctaacgagcc attgttcttt gaaaactgaa taaaagtaat
    13561 cgaatacgtt tcgataagaa aagagccaac ggaactaacc gccatgacct atcttataaa
    13621 gatagcgagc gattcatgtt agtgatccga gaagcaatct ttagcaggat tgcctgcaac
    13681 gacattctta tcgtgataat gatactccca tacagtcaat agtccgagcg tgataaaacc
    13741 gtcgcaggca atgagtatgg ctacatgaga accatgcagg ggtggaactc ccgtgagctt
    13801 tgctaaagct gttcgattgc tggtaaaaca acttttatga aatccaaata agtgatttgg
    13861 aaaggaggat tttatgaagc agactgacat tcctatttgg gaacgttata ccctaaccat
    13921 tgaagaagcg tcaaaatatt ttcgtattgg cgaaaacaag ctacgacgct tggcagagga
    13981 aaataaaaat gcaaattggc tgattatgaa tggcaatcgt attcagatta aacgaaaaca
    14041 atttgaaaaa attatagata cattggacgc aatctagcgt cgccaaaggg tcttgtatat
    14101 gataaaatag tattaagtcg tatcaaggct ctttccataa aggaaaggag caaatgccat
    14161 gtcagaaaaa agacgtgaca ataaaggtcg aatcttaaag actggagaga gccaacgaaa
    14221 agacggaaga tacttataca aatatataga ttcatttgga gaaccgcaat ttgtttactc
    14281 gtggaaactt gtggctacag accgagtacc agcaggaaag cgtgattgta tctcacttag
    14341 agagaaaatc gcagagttac agaaagacat tcatgatggt attgatgttg taggaaagaa
    14401 aatgacactc tgccagcttt acgcaaaaca gaacgctcaa agaccaaagg ttagaaaaaa
    14461 cactgaaact ggacgcaaat atcttatgga tattttgaag aaagacaagt taggtgtaag
    14521 aagtattgac agtattaagc catcagacgc taaagaatgg gctattagaa tgagtgaaaa
    14581 tggttatgct tatcaaacca tcaataacta caaacgttct ttaaaggctt cattctatat
    14641 tgctatacaa gatgattgtg ttcggaagaa tccatttgac tttcaactga aagcagttct
    14701 tgatgatgat actgtcccta agaccgtact aacagaagaa caggaagaaa aactgttagc
    14761 ctttgcaaaa gctgataaaa cctacagcaa aaattatgat gaaattctga tactcttaaa
    14821 aacaggtctt cgtatttcag agtttggtgg tttgacactt ccagatttag attttgagaa
    14881 tcgtcttgtc aatatagacc atcagctatt gagagatact gaaattgggt actacattga
    14941 aacaccaaag accaaaagtg gcgaacgtca agttcctatg gttgaagaag cctatcaagc
    15001 atttaagcga gtgttagcga atcgaaagaa tgataagcgt gttgagattg atggatatag
    15061 tgatttcctc tttcttaata gaaagaacta tccaaaagtg gcaagtgatt acaacggcat
    15121 gatgaaaggt cttgttaaga aatacaataa gtataacgag gataaattgc cacacatcac
    15181 tccacatagt ttgcgacata cattctgtac caactatgca aatgcaggaa tgaatccaaa
    15241 ggcattacag tacattatgg gacatgctaa tatagccatg acgctgaact attacgcaca
    15301 tgcaacattc gattctgcaa tggcagaaat gaaacgcttg aataaagaga agcaacagga
    15361 gcgtcttgtt gcttagtagt acaaatgaat ttactactta tttaccactt ctgacagcta
    15421 agacatgagg aaatatgcaa agaaacgtga agtatcttcc tacagtaaaa atactcgaaa
    15481 gcacatagaa taaggcttta cgagcattta agaaaatata aaaagataat tagaaattta
    15541 tactttgttt aatttaaaaa atatccagat aaaaacttta gtaatttaga ttattgctta
    15601 ataggaagta aatgttcaaa tagttttata aaagaaaagt tgattacttt ttttaaagtg
    15661 agaataccag atatattaaa agacaaaagt atattaaaag cagagttatt tattcatatt
    15721 gattcaaata agaatcatat ttttaaagaa aaagtagata ttgaaattaa aagaataagt
    15781 gaatattata atttacgaac tataacatgg aatgatagag tgtctatgga aaatatcagg
    15841 gggtatttac caattggtat aagtgataca tccaactata tttgtttaaa tattacagga
    15901 actataaaag catgggcaat gaataaatat cctaattatg ggttagcttt atctttaaat
    15961 tacccttatc agattcttga atttacatct agtagaggtt gtaacaaacc gtatatactt
    16021 gtaacatttg aagatagaat tatagataat tgttatccta aatgtgagtg tcctccaatt
    16081 agaattacag gtccaatggg accaagagga gcgacaggac caagaggagc aacaggtccg
    16141 acaggaagta caggaccaac gggagaaata ggcccaacag gaaatacggg agcaacagga
    16201 gcgacaggaa gtataggacc aacgggag
//
LOCUS       DAEMAE010000474         5217 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.236, whole genome shotgun sequence.
ACCESSION   DAEMAE010000474 DAEMAE010000000
VERSION     DAEMAE010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.236"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..232
                     /locus_tag="KPH93_003883"
     CDS             <1..232
                     /locus_tag="KPH93_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG1690576.1"
                     /translation="YLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLTEPIEGPIRS
                     IMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            242..1027
                     /locus_tag="KPH93_003884"
     CDS             242..1027
                     /locus_tag="KPH93_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG1690577.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILSLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            1062..1589
                     /locus_tag="KPH93_003885"
     CDS             1062..1589
                     /locus_tag="KPH93_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG1690578.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    1764..1996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2088..5195
                     /locus_tag="KPH93_003886"
     CDS             2088..5195
                     /locus_tag="KPH93_003886"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG1690579.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLANKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFDEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
BASE COUNT         2096 a          550 c          981 g         1590 t
ORIGIN      
        1 ttatctttta gatataatag catggatgat agttataaaa agcttgatga cttggtttcc
       61 aaatggaaga caaagtaaaa tttttgatat tttggaaaat cttacagagc caatagaagg
      121 gccaataagg tctataatgt ataaatatac aaatggacct atagattttt ctcctttaat
      181 tgcaatagta ttattgatgt tcctaagaca attagtattg gttatattct aaggtgatag
      241 gatggataaa ttaaaactaa caaatcacat aaaggatata gacttgaaaa ataaaatgtt
      301 taaagtgata gacaaggcta atagttgtat aaaaaactat gatgttaagt ctacagagtt
      361 tcttaatcca tatgaagtga aaaatgctgt agcaatatta aattctacaa atgaaataaa
      421 atacagtgtg gatggaggtt atgaacaagc tgaaagaagc actgtattta tttatccatt
      481 ttacatggaa tacgaagata tagaagatac tcttagattt ttacaaatag aagggaattt
      541 caaatttaaa aatatatctc ataaagatta cttagggtca atactgagtc taggtataaa
      601 aagagaaaaa attggagata taattataca tgatagcttt tgtcaagtag ttgtaagtag
      661 tgatatttgc gattttataa ttgtaaactt agaaaaggtt tcaagaaata atgtaattgt
      721 aaaggaaatt tccagagaaa atattgttaa tagttcttca aaatataaag aagtatcatt
      781 tacagtatct tcagataggt tagattgtgt gataagtggt atttacaata tatctagaca
      841 agatagtgca aaatacataa atggagaaaa agtccatgtc aattatgaaa agataacttc
      901 aacttctaaa atagtaaaaa atgatgattt gatttctatt agaggcaaag gaagagctaa
      961 ggtaacacaa ataggagata ttactaaaaa aggaaaaata aaagttcaag caagattaat
     1021 tgtataagga ctaatttaaa ttatagctgg gaggaaatgt aatgctaact ccaattgaga
     1081 tagaaaataa agagtttaaa aaaggtttaa gagggtatag agatgaagag gttgatgaat
     1141 ttttagatat catcaaggaa gattatgaat ctctatgtag ggaaaataca gctcttaaag
     1201 aaaaattagg gttatatcaa gaccaagtaa acaagtatga aaatattgaa gaaacactaa
     1261 aagctacttt aataactgct caaagtgcag cagaagacac ttgtagcgcg gctaataaaa
     1321 aggctaagat aattgttgaa gaagctgatt taaaagctag acaaattata gaacaagcta
     1381 ataaccgtgt tatagaaata agaaaagagt atgatgcttt agtcaaagaa tttaagatat
     1441 ttagaaataa atttaaatca ctgttggaag atgaaataag aagtgttgat gagatttttt
     1501 atgatgtaga tgaaaaatgt gtaaatgcat ttgaaggaac aacagtgtat aattatagta
     1561 atgatgaagc tgctacaact ttagaataag cttgtaattt gtttattttt tatgtagaat
     1621 cattgtgtaa gtttgagaaa tctataacaa agattaaaaa taggataaaa atgtaagaaa
     1681 aatagaaatt attatatttt tcaaaaatat attgactatt caaattattt gttatagaat
     1741 ttcatataaa cacagataaa tttttaaaag cgttgataga gacatttatc tttagataat
     1801 tttacagaga attgggtatg ctgagaacca atgaatttat aaagataaaa atcactcttg
     1861 agctgcaggc tgaacgatta aattctacta agctgtgccg gttagtaccg ttatactatt
     1921 agagtgttat atgtagctag tatttagttt acatatagaa ttagggtggt aacgcgattt
     1981 ataactcgtc cctttggttt aaagggggcg agttttttag tatttaaatt tatgtgaaag
     2041 aataaaaaat tttggaatta taatatttat aggaaagggt gatttacatg gcgaaattta
     2101 agccattggt agacagttct gtaaaacaag cagaagcaca agtttttgat tattggaaag
     2161 acataaatat acttgaaaaa actttagaaa aagggaaaga tgacccaagt tttgtattct
     2221 atgaaggacc tccaacagct aatggaaatc ctggagtaca ccatgtttta tctagaacac
     2281 ttaaagattc agtttgtaga tataaaacaa tgtctggtta tcaagtaaag agaaaagcag
     2341 gttgggatac acatggatta ccagttgaaa tacaagtaga gaaagagtta ggtcttacta
     2401 gcaagcaaca aatagaagag tatggaatag ctgaatttaa ccaaaaatgt agagagtcag
     2461 ttttctcatt tgaaaaacaa tggagaataa tgactgaaag aatggcttat gaagttgatt
     2521 tagataatcc atatataact ttagataata actatataga gtctgtctgg tggatactga
     2581 ataaattcaa caaagaagga tatatctatg aagggcataa aatacttcct tattgcccaa
     2641 gatgtggaac tggtcttgct agtcatgagg ttgcacaagg atataaagaa gtaaaaacta
     2701 atacagttat agctaaattt aagaaaaaag atgctgatga atacttctta gcatggacaa
     2761 caactccatg gactctacca tcaaatgttt cgttaacagt aaatgctgat gtagattatt
     2821 taaaagttaa aaaaggtgat gaggtatatt atgtatctaa acctttagct aataaagtat
     2881 taggtgaaga ctatgaagtt ttagaagaaa tgaaaggtaa agacctagaa ggtttagaat
     2941 atgaacaatt aatgcctttt gtagaagttg acaaaaaagc attttttatt acagtaggag
     3001 attatgtaac tactgaagat ggtactggta tagttcatac agcaccagca tttggtgaag
     3061 atgactacaa cacaggtaaa agatatgatt taccagttat tcaaccagta gatgaaactg
     3121 gtaaatttac atctactcct tgggaaggta gatttgtaat ggaagatggg ctagatgttg
     3181 aaataataaa atggctagct tctgaaaata aattatatag caaagaaaaa gtagttcata
     3241 attatccaca ctgttggaga tgtcaaactc cactagtata ctatgcaaaa ccaagctggt
     3301 atatagagat gactaaactt aaagataaat taatagaaaa taataatggt gtaaaatggt
     3361 tcccaagttt tgtaggagaa aagagatttg gaaactggtt agaaaatcta aatgactggg
     3421 caatatcaag aactagatat tggggaactc cacttcctat atggagatgt gaatgtggtc
     3481 atacagattc agttggttca agagctgaat tagctgaaaa agctatagaa gatgtaaatc
     3541 cagaaacagt tgaacttcat agaccatatg tagatgatat acacttaaaa tgtgaaaagt
     3601 gtggaaaacc aatgacaaga gtaacagagg ttatagactg ttggtttgat agtggggcta
     3661 tgccatttgc tcaacaccac tatccatttg aaaataagga aaactttgac caactgttcc
     3721 cagctgatta tatatcagaa ggtatagacc agactagagg ttggttctac tcattacttg
     3781 cagtatcgac atttgtatta ggaaaagcac catataagag tgttttagtt ccagaccttg
     3841 tacttgataa agatggtaag aaaatgagta aatcaagagg aaacacagta aatccaatgg
     3901 aattatttga ccaatatgga gcagatgcat taagatggta tttactatat gtttctccac
     3961 catggactcc aactaaattt gatatggatg gattaaaaga aatccaaagt aaatttatag
     4021 gtactatgaa gaatgtatat aatttcttta ctttatatgc aaacacagat gatataaatc
     4081 caactgaatt ttttgtagaa tataaagata gaccagaact tgatagatgg atattatcta
     4141 aattcaataa tttaatgaaa gatgtagaag aaaatttagc tatatttgag ttaaataaaa
     4201 ctttaagaat gatacaagat tttataaatg atgatttatc taactggtat ataagacgtt
     4261 caagaagaag attctgggca actgaattaa cagaagataa aaaagcagtg tacaatacaa
     4321 cttatgaatt gttacatggt ctttgtagag ctatagctcc atttgcacca tatatgtcag
     4381 aagaaatgta tagaaactta acaggagaag tatctgttca tttagctgaa tatcctaagt
     4441 gtaatgaaga gttagtagat actaaattag aagaaaaaat ggatttagct aaaaacttag
     4501 ttactttagg tagagcttca agagaagtag agagaataaa agttcgtcaa ccattacaaa
     4561 aagtgttagt agatggtaag tttgaagata caataagtga tgtagttgat ttaataaaag
     4621 aagagcttaa tgttaaagag gtaatatttg ctaaggattt agatgagtat atgaacttta
     4681 gtttaaaacc aaactttaag gaagcaggac cattattagg ttctaagatg aatttattcg
     4741 ttggagcatt aagtaaatta aatgctcatg aaactgctaa taaacttgaa aaaggtgaga
     4801 ctttaacagt tgatttagat ggtgaagctt ttgaatttaa taaggattta gtattaatag
     4861 gtataactgc taaggaagga tttaatgtat ctgttgaaaa taatcttttt gttatactag
     4921 atactaagct aactgaagag ttgattaatg aaggttatgc tagagaattt atatctaaag
     4981 tacagcaatt aagaaaatct aataactttg aagtattaga taatatagta atagaatact
     5041 gtggtgatga tgaaattgct aaggctgttg accattttga tgaatacatc aagtctgaaa
     5101 ctttagcttt agaaataaac agagtagatg ataaatcttt agaggaacaa aacttaaatg
     5161 accatatgac aggtataaaa gttataaaaa aataaaaaga atagttagtt aaccaaa
//
LOCUS       DAEMAE010000475         4606 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.258, whole genome shotgun sequence.
ACCESSION   DAEMAE010000475 DAEMAE010000000
VERSION     DAEMAE010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.258"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            357..1055
                     /gene="vanR"
                     /locus_tag="KPH93_003887"
     CDS             357..1055
                     /gene="vanR"
                     /locus_tag="KPH93_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG1690580.1"
                     /translation="MNGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQLRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            1091..2206
                     /locus_tag="KPH93_003888"
     CDS             1091..2206
                     /locus_tag="KPH93_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1690581.1"
                     /translation="MKKINNFTYRKLRDKYEFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPDMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            2351..3238
                     /locus_tag="KPH93_003889"
     CDS             2351..3238
                     /locus_tag="KPH93_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1690582.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYTMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            3239..4474
                     /locus_tag="KPH93_003890"
     CDS             3239..4474
                     /locus_tag="KPH93_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1690583.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DDNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDVYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAIAAPTF
                     SGEYQTQSDSILRCTKHGHIRLAFTKILALFSIFIIMFVICISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
BASE COUNT         1712 a          555 c          745 g         1594 t
ORIGIN      
        1 ttattataaa tgtgaaatgc taacgatatc taaactaaat tattataaaa tagctgactg
       61 taatttaatt gataaagcct attattctaa actttgccag ttaaattaca gtcagtttta
      121 ttatataaaa aatacgtaac ggaagtatta ttttatagat tatatttgaa aagataaata
      181 aattgtatat ctaaatctat atattatttt tttatcttta atatatatta aataataaaa
      241 tagttaaata ataagattaa gaatttctta ataaattcta atgtaaaaaa ttatataaaa
      301 aaaattaaat ttagtataat atatataaat atatatagaa aggatgttta aagtctatga
      361 atggaaaaat tttagttgta gatgacgaac gtgatatagc tgatttaata gaagtctacc
      421 ttacaaatga aaactatatt gtttataaat tttattctgg tcttgatgca ctaaactgca
      481 ttaatttaaa tgaatttgac cttgcaattt tagatgttat gttgccagga gtaaatggat
      541 tttctttatg ccaaaaaatc cgagaaaaat atacttatcc aatcattatg ttaacagcta
      601 aggatgaaga aaccgacaag attacaggct taactttagg tgcagatgat tatgttacta
      661 aacctttccg acctttggaa cttgttgcta gagttaaggc acagcttaga agatataaga
      721 agtacagtgg aagtttacaa tctggtgaag aactttcaca tattttttct tttttaggtc
      781 tggaattgaa tacccaaaca tatgaatgtc atttaaatgg taaagctctt tcacttactc
      841 caacagagtt ttctattcta catgtattat taaaaaataa aggctctgtg gtaaattcag
      901 aaaatctttt tcatgctatc tggaaagatg aatattacaa taaaagtaac aatactataa
      961 cagttcatat tcgccatctt cgtgaaaagt taggtgatac tgtagatgaa gcaaaatata
     1021 taaaaacaat ttggggtgtt ggttacaaga tttgacacct atttttcttg tcaagaaagg
     1081 atgtagtatt atgaaaaaga ttaataattt tacttatagg aaactaagag ataaatatga
     1141 atttataaaa agagaaaaaa atacagaggc gtatttaaag tataaattat atataatgtt
     1201 agctatctat acaggtatag tattggtttt aatgtttatg gctgattata cgttttcaaa
     1261 tttttcaaat cctcttgtta aatggcttaa gttgagaaga gatgtattat tttttttggt
     1321 ttttataatt ggatatatca ctattttcaa tttttattgg aaaaaaccat ggaattattt
     1381 aagagaaatc atcaatgcaa cacatactat ttatcaacaa aatgatgaat tggttgaact
     1441 atctaagcca ttaaaggaaa tagagcaaca aatgaaccaa attaagatgt ctgttttgtt
     1501 aagtcaacaa gcagtgaaag aggctgaaaa taaaaagaat aaccttgtga tgtatctagc
     1561 acatgatata aggacaccac ttacttcagt tattgggtat ttgagcttat taaaagaaat
     1621 acctgatatg gataattctc aaaaatctaa gtatactgaa gttgcacttg acaaagcaca
     1681 atatttagaa aaacttatca atgaactttt tgaaataaca cgatataatt ctaatcaaat
     1741 caaaattaat ttcgaaaaag tagacctata ttatatgtta cttcagttaa aggatgaatt
     1801 ttacccattg ctatctgaaa aaggaaatag agccattcta aaagcaaatg aaagtttaac
     1861 aataaaagca gattctgaaa aacttgcaag agtatttaat aatatcttga aaaatgctat
     1921 tgcttacagc tatcctaata cagaaattat tatctcagcg gttcataaag acagcaaggt
     1981 agatattgct tttagaaatt atggacagac aattccggaa gaacaacttt ctgctatatt
     2041 tgaaaagttc aatcgtttag atgaagctag aaaatctgat atgggtggtg caggtttagg
     2101 gctttctata gctaaggaaa ttataaattt gcatggtggt gatattactg ttcaaagtaa
     2161 agatgaagtc acaacattta ctatatcgct tcctacatct atttagtaaa atataaatca
     2221 aatattagga ttttcttaag aaattatcta tttaatatta aaactatgag tgtctttgtt
     2281 tagtaaaata aatactgagc aaggcacttt tgtttttgca ggtaagtata taaagagagg
     2341 agtttcaaat atggaattaa aaattgaaca tgtgacgaaa aaatttaagg ataaaattgc
     2401 tgtagaagat gtttgcctta ctttaacacc aggtgtgtgg ggatttctag gagcgaacgg
     2461 agcaggtaaa actaccctta tgagaattat tacagacatt atgaaaccta ccaatggcaa
     2521 aattctgtat aatggaaaaa atatttatac aatgggtgct gagtacagaa atttactggg
     2581 atttttacca caagaatttg gtttcccaca ggatttttct gtaaaagatt atttagaata
     2641 tatagcagca ttgaaagatg taccaccaag agaagtaaaa ggaaaaatta ataatctatt
     2701 ggaaatcctt acattatctg atgtaaaaaa taaaaagatt attaaattat ctggtggaat
     2761 gaaacgacga gtgggaattg cacaagccat gttaaatgac cctaaaatcc ttataatgga
     2821 tgaaccaaca gcagggcttg accctggtga gcgagtgaga tttcgtaatt ttatttcaga
     2881 attttctcat aatcgtgttg tcctgatatc tactcacatt gtatctgata ttgagtacat
     2941 atcaactcaa aatgctatta tgaaaagtgg aaaaattctt gatgtgggaa caaccgataa
     3001 actggttaag aaagtagagg gaaaagtatg ggaatgtctt atatcaaata gagatatttc
     3061 aaaatatgaa atgaatttac gtataatcaa tcagcgtagt gaaaaaaaca atcaagtatc
     3121 tattcgttac ttggcagaaa agtcacaaat tgataactct attgctgttg aaccacgatt
     3181 agaagattta tatttatggt tatttccaga agcagtttat agtaaggagg ggaaataaat
     3241 gcgtattttt tatttggaaa ttaaacgagt gttgcactca cgtcgtacat taattttatt
     3301 attgttggcg ttggttttat ctgcattgat ggcttttctt ccaattactt atgagggtat
     3361 caattattta gatgataatg gcaaggtagt tgaattgagt ggtttagatg ctattaaata
     3421 taaaaaaaca atttatgcta ataatagtga aattagtagt ggagttgtaa caccaaaaaa
     3481 attaaagcat gctttaaata tatggaaaaa aacagaagaa aagtatggta aagtaggtag
     3541 tgaagatttt ccactagatg tttattctaa aagcatttta ccaattcgcc cattgttgaa
     3601 tagacttcct gaagcatttg ctaattcaga aacaggattt gcaacagaat tatcagaaat
     3661 aaatacaaat gatttagatt ctttttatga acaatgcaaa actcatttaa atgatattat
     3721 gaagattgaa cgacaaactt ccagtgcaca aatgaaagct caagaacttt atagaaaagt
     3781 agatactcca tttgagatat acccaggtta ttctaaagat gctttcgatt atataatatt
     3841 ttatattttt atattattaa ttatatgtgt tgcaattgct gcacctactt tttctggtga
     3901 gtatcagaca caatcagata gcatccttcg ttgtacgaaa catggacata ttcgtctagc
     3961 gtttactaaa atattagctc tattttctat atttataata atgtttgtta tatgtatttc
     4021 tatccatttg gcaatttctg atttagcttt tggtactgaa tgtttgaaaa cctcatttca
     4081 aatgttgttt tctgtcatta gtctagtaaa tataaattta ctacaagcac agattcttat
     4141 agctattggt ggtctaattt caatacttgc aatggttagt ttaacattat ttttatctgc
     4201 aaaatgtaag gattctctta cttctatgct gattgctttt tctatttgct taattcctat
     4261 gggaataggt gttagttgga ttggatacct attaccgcca gggggaattg gttttaagat
     4321 tagtttatta tatcaaatga catcatttaa tttcttacac ttaggaggta tgagtttttg
     4381 gacaccacac attatactgt ttttctcggt gatagaaata cctattttta tatttttggc
     4441 tattcgtacc tattgcaaac atcaagtatc gtaacaattt gatggaaata atagtgatat
     4501 aaactagagg atacaataat tatatatgta gtataaaagt taaatttagt taacaaatct
     4561 taatagcaga tataggtaat aaattataca taaactgtat tattat
//
LOCUS       DAEMAE010000476         3220 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.308, whole genome shotgun sequence.
ACCESSION   DAEMAE010000476 DAEMAE010000000
VERSION     DAEMAE010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.308"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(102..836)
                     /locus_tag="KPH93_003891"
     CDS             complement(102..836)
                     /locus_tag="KPH93_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBG1690584.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     NYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            962..1678
                     /locus_tag="KPH93_003892"
     CDS             962..1678
                     /locus_tag="KPH93_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1690585.1"
                     /translation="MKKFKLIGAIILVVFISVFISKERLLKKDKDPIVVAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1812..2504
                     /locus_tag="KPH93_003893"
     CDS             1812..2504
                     /locus_tag="KPH93_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1690586.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKN
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFNGSNNEDKDVIEVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2494..>3220
                     /locus_tag="KPH93_003894"
     CDS             2494..>3220
                     /locus_tag="KPH93_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG1690587.1"
                     /translation="MKNKSLLNHMFRPILDLIISCGLTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIE"
BASE COUNT         1308 a          354 c          510 g         1048 t
ORIGIN      
        1 gataataaca tataataaat aaaaaagtaa gattgtgtag attatataaa gattatttac
       61 aattaataca atcttgcttt tttatttaaa aattttaatt attatttaaa attttttatc
      121 ttctctttat cccatataaa caatttctca gaattattta aagctccaaa tacatttctt
      181 ttaaaatcag gcatctcttt atttaaatta gcaataagat tttttatgta ctctctccta
      241 gtatgaccat ttgcagggca tggatttgtg ataataggat agttagaatc ttttgaaatt
      301 ttcttagtca tatactcttc tatgtaaatc ataggtctta taactgttaa atcctgtcta
      361 tctaggtatg tctttgggga aaaacaattt actctaccct cataaaacat agacattaaa
      421 aatgtctcaa ttgcatcatc tttatgatga ccaagagcta ctttattaca tccaagtttt
      481 ttagcattat cgttcaaagc acctcttctt aaattagcac ataatgcaca aggattcttt
      541 tcttgtctta tatcaaatac aatttctttt atgtcagttt gaaattcata aaaaggaaca
      601 tttatttctt tacacaattt atctagtgga gaattatcaa cacctccggt atttaatgtt
      661 atggctatca attcaaagtt ttgtggagaa aatctttgat aatttttaag tatatgaagt
      721 agagttaaac tatcttttcc accagaaagt ccaaccgcaa ttttatcatt ttcttgtatc
      781 atatcaaaat cttgtatagc cttacgagct ttacttagaa gtttttgtat agacatttta
      841 tcaccatcct ttaaaaatat taaatttaag atttatttaa gatttactta taaaatactt
      901 aagtcttata ctctaaaatc aacattatag acaaacataa caaaaaggag aatatgtaat
      961 tatgaaaaaa tttaaattaa taggagccat tattttagta gtgtttataa gtgtatttat
     1021 atctaaagag agattactta agaaagataa agatccaata gtagttgctc aacatgattc
     1081 agaacaagat gttgaggcaa attctcaaaa taaaaatcac tcaaatagta tatcaaatat
     1141 attattggta aacaagacaa atggaattag caaaaactat actcctgaaa atattactaa
     1201 ggtaaacata ccttttgttg aagaagcaac agaggaagaa aaacaaatgg caggagaacc
     1261 tgcaaaggca gttgaagatt tagttaagca agctaattca gaaggaatac agtttttagg
     1321 gtcttcagca tacagatctt atgatactca actggatact tatacaagaa gggtaaaatc
     1381 tcaggggcga gaaaaggcag atgcatatgt agcaaagcct ggttacagtg agcatcaaac
     1441 tggactttgt attgatttaa caaatccaga aagatggttt gttggaagta caaaagaagc
     1501 taaatggttg gcagaaaatg ctcacaagtt tggatttata ataagatatc ctgaaggtaa
     1561 agaagatata acaggtacag catatgaacc atggcatatt agatatgttg gtaaggatgc
     1621 tgccgaggaa atttattcaa aaggattaac tttagaagaa tatttacaaa ataaataata
     1681 taaatgtgta aataatttgt attatattgg aaatttagaa atgaataaat ttatagatta
     1741 cactaactaa aatatagtat aattttatat gagtaaagtt atatacaaag atgaagttag
     1801 gatgtgtaaa aatgaacaat acagttcttg ttgtggatga tgaaaaggaa ataagagacc
     1861 ttatagagat atatctttta aatagtggat ataatgtaat taaagctgaa gatggaaaag
     1921 aggctctaga tattttaaaa aatgaaaata ttcatttaat ggtattggat ataatgatgc
     1981 ctaaaatgga tgggatagaa gtttgtagaa aagttagaga aagtttgaat ataccaatac
     2041 ttatgttaag tgcaaaatca gaagacatgg ataaaataca agggataatg actggtgcag
     2101 atgattattt gacaaaacca tttaacccac ttgaacttac tgtgagagtg aaatcactga
     2161 ttagaagagc atattatttt aatggttcta ataatgaaga taaagatgtc atagaagttg
     2221 gagtagtaat tatagataag gaaaaacata gtgtaacagt agctggaaat gaaataattt
     2281 tgacatcaag agaatttgat atattatatt tactagcaaa caataaaggc agagtatata
     2341 gtacagaaga aatatttgaa aaggtatgga aagagaaata ttaccaatct aataatactg
     2401 taatggttca tatgagcaga ataagggata aaattgaaaa atatacagat ggagaaaaaa
     2461 taattcatac tgtatgggga gtaggatata aaattgaaaa ataaaagttt attaaatcat
     2521 atgtttcgac ccatattaga tcttataata agctgtggtt taacttcaat gcttatttta
     2581 acaagtatgt atataataaa attattatat ttcaatagtg agatatctgt aattaatcaa
     2641 cttgtatatc agttaagaga tttcagaagt cttataggag agagtttatt ttatccattt
     2701 ttatttacaa tcacatcatt agcaatttat tccatgatat catacaagag aaataagcaa
     2761 attgtagatt ttataagaaa gactaataga aaaaatcaaa aattagaaat cattaaaagt
     2821 gctgtaaatt taatggatga aggtaactta gaaaaaagta tagatattga gtatgactta
     2881 gatatattaa aggataaaga aaaggatgat attgatgaac tagcacataa aatcaataat
     2941 atagtccatc aacttagaaa tataactatt gaagagagac aagctcagca gacaaaaaca
     3001 gatttaataa caaatgtgtc acatgactta aggacacctt tgacatccat aatgggttat
     3061 ttaggagtga tagaagaagg taagtataaa gatgaagttc agatgatgta ttatgttgat
     3121 attgcatatg aaaaatcgaa aaatttaaat gttcttataa atgatttgtt tgaattgact
     3181 aaaatgcaaa ataatacaat aaaattaaac aaaatagaaa
//
LOCUS       DAEMAE010000477         2647 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.335, whole genome shotgun sequence.
ACCESSION   DAEMAE010000477 DAEMAE010000000
VERSION     DAEMAE010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.335"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..606
                     /locus_tag="KPH93_003895"
     CDS             <1..606
                     /locus_tag="KPH93_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HBG1690588.1"
                     /translation="HFYYFSKMTNVGFNLTIEIDITNLLKETKKRNIKFYPAYLYTIT
                     TCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSSIWTEYDENFSIFYENYLA
                     DVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNHGLENYFFPTIEAGKYYQK
                     DEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWLH"
     gene            complement(818..>2647)
                     /locus_tag="KPH93_003896"
     CDS             complement(818..>2647)
                     /locus_tag="KPH93_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1690589.1"
                     /translation="GENNIISITHCATRLRVMVKDREIINDKKVEKVNEVKGVFFTSG
                     QYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFKKVMRTLADIFVPIIP
                     VIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLTETAFAFLPAIIVWSA
                     FKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFGFIPIVGCQGSVLTAI
                     VTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVGPIMHTIELKMVDIIS
                     LLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGFNALITLCAMYGFANI
                     GTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYNLKPLIIMCASSGLGA
                     AILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVVFCFIATCLFGVPKEA
                     INEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLGDGFAIDIIDGKIVSP
                     ISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVELNGDGFDVAVKQGDMVKQGDVLVN
                     VDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE"
BASE COUNT          967 a          468 c          317 g          895 t
ORIGIN      
        1 catttttact acttttcaaa aatgacaaat gtagggttta atttgactat agaaatagat
       61 attactaatt tgcttaagga aacgaaaaaa agaaatatta aattttatcc agcgtattta
      121 tatacaataa ctacttgctt aaatcgatat agagaactaa aaatcgcata tgatgaagat
      181 gaactcggat attggaacac gttaacgcca ctttatgcga tttttcatga tgataataag
      241 actttctcat caatatggac agaatatgat gaaaatttct caatattcta tgaaaattat
      301 ttagctgatg ttaaagaata tggaaaaaat catggttttc tagcaaaacc aaatccgcca
      361 agaaactgct atactgtttc ctgccttcca tggattagct ttaagagttt tactgtacat
      421 aatcatggtc ttgaaaatta ttttttccct acaattgaag caggtaaata ctatcaaaaa
      481 gatgaaaaaa tacttcttcc tctttctata acagtgcatc acgcaactac tgatggatac
      541 catgtaagtt gttttattga ttacatacaa aaaatgtgct ttaatactaa gatatggcta
      601 cactaaaaat ttatacaata taaaaagaac tgtcgcatga gcaaattaaa tttgctatga
      661 gaaagctctt ttttacttac caatagttaa tatgagatga attatttctt ataccttgaa
      721 cttaccaata gttaatatga aatgaattat ttctcatact ttgaaaatat tgaattgatt
      781 ttgataatct tcttttataa tcattacgtg tcacctacta ctcaacacat attctttgac
      841 ctatttttac atccttgttt atatccaaaa tatttacctt cttaccatct ggaaacaata
      901 ccattgtcaa agtagacttt cctaattcgt gtattctctt taaatcaaca ttaacaagaa
      961 catccccctg tttaaccata tctccttgtt taacagcaac atcaaagcca tctccattta
     1021 attcaactgt atcaattcca acatgaatta atacttctcc attagttcct ttaatcccaa
     1081 atgcatgtcc acttgaaaat actgtttcta atttacctga aataggggaa acaatttttc
     1141 catctataat atcaatagca aaaccatcac ctaacatttt tttagaaaaa gtttcatcag
     1201 gtacattttc taatgcaaca atcttacctt tagctggaga cacaaagttt tcattatttt
     1261 cattaaacac taattcttcg tcttcatctt catttatagc ttctttaggt actccaaata
     1321 aacatgttgc aataaagcag aatacaacta caaatattga tactaataag tatgtgatta
     1381 aattatatcc atcatagata tacattaaat aagaaggtaa aactgctaat ccatatgaat
     1441 tagattgaat atgtaaaatt gataatattg ctgctcctaa acctgaagat gcacacataa
     1501 taatcaaagg ctttaaatta tatcgaagct gaatcccgaa taaaacaggt tcacttatac
     1561 caaaaagttg agatagcatt gctccaactg cagtttgttt aacttgatta tttttagatt
     1621 ttttcataaa tgctagacat gttccaatat tcgcaaatcc atacattgca caaagagtaa
     1681 taagtgcatt aaatcctgta tttgctaata gtgatgtttc aatcattaca taagtatgat
     1741 gtaagccagt aataacagct aatggatatg taaatccaat aataaatcct ccaattccta
     1801 aaggtaaacc aatcaataag ctgatgatat caaccatttt taattctatt gtatgcatta
     1861 taggccctac acctaacaaa ataactaaca ttgttattag cataacaaag aatggtgtga
     1921 aaataaggtc taatacatta ggcatatgtt tacggaacca tttttcaaga ttagctccaa
     1981 taaatgctgt aacaatagct gttaaaacac ttccttgaca gcctacaata ggaataaatc
     2041 caaatgccat aattgcttca acttcatttg atgggtcagc aacagaatat gcattaggaa
     2101 gtattgggga aactaacata agtccaataa caagtccaat aacaggtgtt cctccaaata
     2161 ccttaaatgc tgaccataca ataattgctg gtaaaaaagc aaatgctgtt tctgtaagaa
     2221 ccgaaacaag tgtctgaata tacaatggaa tatttgcaga agacattcca aataatccaa
     2281 gtacattatc attaaacaga caacccttta atcccaagaa taatcctgtt gccgcaataa
     2341 ctggaataat aggaacaaat atatctgcta gagttctcat aactttctta aatccagttt
     2401 cattattttt aactaattca tcttgttctt tttttgataa agttttcaat cccatttttt
     2461 caacttctgc ataaacttta ttgactattc ccgttcctaa aataatttga tactgtccag
     2521 aagtaaagaa aacaccttta acctcattaa ccttttccac tttcttatca ttaataattt
     2581 ctctatcttt aaccatcacc cttaatcttg tggcacaatg tgtaatagaa ataatattat
     2641 tttcacc
//
LOCUS       DAEMAE010000478         2505 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.343, whole genome shotgun sequence.
ACCESSION   DAEMAE010000478 DAEMAE010000000
VERSION     DAEMAE010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.343"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(340..1107)
                     /locus_tag="KPH93_003897"
     CDS             complement(340..1107)
                     /locus_tag="KPH93_003897"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG1690590.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIPDIPLLFTFRSVVEGGAKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELSADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            1453..1674
                     /locus_tag="KPH93_003898"
                     /pseudo
     CDS             1453..1674
                     /locus_tag="KPH93_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725701.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1914..>2505
                     /locus_tag="KPH93_003899"
     CDS             1914..>2505
                     /locus_tag="KPH93_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG1690591.1"
                     /translation="MTIPNLNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSNSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGIHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSN"
BASE COUNT          955 a          378 c          264 g          908 t
ORIGIN      
        1 ccattaatat acataattac aaaaaaataa tgacaaatat acataataca aatatatctt
       61 ctactttata taaaacagtt attatgaaaa cttactatct ataaataata gaaatgctag
      121 tataccatct ataatctaat gtctagtagg ttttaatggc atattttaaa tatgccatag
      181 ttgatttata agattttcta tatcactttc aaaatacaca ctaaatagtt gcttttcaga
      241 tataattgcc caatttaaat tttactatct gaaaagtttt ttatatttaa tttatatctt
      301 caaaaattaa ataccttcta atatttctat ataattaaat taattaatac tcttatgtaa
      361 taaattaagt actgaattta actctttaaa tgatatttgt cctggcgctg aaacactttt
      421 tgcagctcca aaagtcaatg cagaaccaaa tatttcacca cacaatctac ttataacgcc
      481 cattcctgac attgacattg ttattattgg tctatctgca taaattttaa acatttcatt
      541 tgtagcttca agtagcacta acacatcttt ttcattttgc ggcataacag ctattttggg
      601 taaatctgcg ctcaattcct gcattctaca taaacgagat acaatctctt ctttttttgg
      661 agttttatta aaatcatgat tagatataat tactttcact tcttttttat gagcaaaatt
      721 aactacttca tctataacct catcccccat aaataactct acatctatta aatctactaa
      781 acctgtatta gaaatttctt tattcaaagt agtataataa tctcttgata ttaattttgc
      841 tcccccttct actacacttc taaatgtgaa caataaaggg atatcaggta tataacttct
      901 taattcatag agaacttcct taacttcttt aatattttct acattctcaa aaaaatcaac
      961 tcgccactct ataatatcca aacatgcatc ttttaactct ttagcctctt ttataatatc
     1021 ttttttattt ttcccgataa ttggtacaca aattttaggt ctaccttctc caatagtaat
     1081 attttttact tgaacttttc ttttcatatt ctcctccata atcagaaatt ctataggact
     1141 ttaaatttta ttttctacac aatttaatta tacattataa atactataca ttataaaaat
     1201 actaatttaa aagtaatttt tattctaatt cttaaaacaa tcccacatat tttagaaaag
     1261 atactctata atatacaaac acttatttct tacctaaatt tattttaaca ttctataatt
     1321 ttcacaatat tccttaaatt ttactcatat aatcaatatt attctgcact ctaatagaat
     1381 taatctataa atcatagtat aattatacag aaataactcg aatttcacca cttatgaagg
     1441 gatttaatat taatgaatct aaatcatttg cattacttca gagttttagc taaagttgaa
     1501 cactatacaa aagcagctac tcaattgtct ataccccaac caagcttaag taatgctata
     1561 tagattctcg aaaaagaatt gagtactttt ttatttgaaa aacaaggtag aaatgtttgc
     1621 ctcaccaaat acggaaggtt tttcctaacc tatgttgata aagcattaag ttaataaaaa
     1681 aaagttgcat ataaaatata ttaaattgtt gcaagtttta ttgttttgat atacaatcaa
     1741 ctaaacatat aatctttata aaatactata cagttaaaag tctgttttta aaaacaaaat
     1801 gtataaatta taaattaaaa tatagatact atcaaggagt gattctgtga agagatgtat
     1861 tgtagtagtg gaatagtcaa aaacataata tatatcaaag gagagattta aatatgacta
     1921 ttccaaattt aaataaggta tatccaagaa gtaatgatta tcaaactgta tatcttaaaa
     1981 atgtaataac aagagaaaat atagaagttg gagattacac aatatacaat gacttttata
     2041 atgacccaag agattttgag aaaaacaatg tattatatca ttaccctata aacaatgata
     2101 aattaataat tggtaagttt tgctcaatag catgtaatgc aaaatttcta atgactagtg
     2161 gaaatcactc aatgaaatca aactccacat atacctttcc catcttttat gaagaatgga
     2221 aactcgatat caaccatata acggacgctt gggacaataa aggtaacatt ataattggaa
     2281 atgatgtgtg gataggttat gattctataa ttatgtcagg tatacatatt ggtgatggtg
     2341 caattattgg aacaagagca gtagttacta atgacgtccc accgtattct attgttggag
     2401 gagtacctgc aaagattata aaaaagagat ttaataatga tacaatttca aaattattag
     2461 aaattaaatg gtgggattgg ccatataaaa aaattcaatc aaaca
//
LOCUS       DAEMAE010000479         1089 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019744
            SAMN15907312-rid12312513.guided.419, whole genome shotgun sequence.
ACCESSION   DAEMAE010000479 DAEMAE010000000
VERSION     DAEMAE010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907312
            Sequence Read Archive: SRR12520653
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050505.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050505.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,900
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,744
            CDSs (with protein)               :: 3,744
            Genes (RNA)                       :: 90
            rRNAs                             :: 9, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 19 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 19 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907312-rid12312513.guided.419"
                     /isolate="M2016019744"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-09-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            3..941
                     /gene="blaCDD"
                     /locus_tag="KPH93_003900"
     CDS             3..941
                     /gene="blaCDD"
                     /locus_tag="KPH93_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG1690592.1"
                     /translation="MKRKNNFIWIVILLVVVAMGIYYVGRKHNNTKQKYDKNYINSEL
                     NKSKIENNDKNKKEKINIVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLIN
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILKDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          472 a           89 c          183 g          345 t
ORIGIN      
        1 gtatgaaaag aaaaaataat tttatttgga tagttattct tctagttgtt gtagctatgg
       61 gaatatacta tgtaggaaga aagcataaca atacaaaaca aaaatatgat aagaattata
      121 taaactctga attaaataaa tctaaaattg aaaacaatga taaaaacaaa aaagaaaaaa
      181 ttaatatagt tgactatagt gattgttttg aaggtattag tggaggagct attttttata
      241 atactaaaaa taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac
      301 catgttctac ttttaaaata gtttcaactc taattggttt agaaaaaggg gtaataaact
      361 caaaagaatc cgttatgggg tatgatggta caaattatcc aaataaaaat tggaataaaa
      421 atcttagttt agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgataa
      481 ataaagttga tgctaaatct gtccaaaata ttttagatga tttaaagtat gggaattgcg
      541 atattagtga atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt
      601 tagaatcttc attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg
      661 agggagatac aaactttaaa aaagagcata ttaatatttt aaaagatatt atgaaaatag
      721 atgtaaatga taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa
      781 ataaacgtgt ggatgcatgg tttgttggta tgctagagcg tgaaggtgat acctattatt
      841 ttgctattaa atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg
      901 caattaatat aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa
      961 tattggttac ctatagtctg taatatttat taaataattt tactaaattg agtctatata
     1021 gagcaaaact cctttagtac ataaaatact tgctattgat tattaaatta aatacctata
     1081 gatttaaag
//
